LOCUS       DAOPQG010000001        67341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_529_length_67341_cov_14.380228, whole genome shotgun sequence.
ACCESSION   DAOPQG010000001 DAOPQG010000000
VERSION     DAOPQG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 67341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67341
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_67341_cov_14.380228"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            866..2938
                     /locus_tag="PKI07_00005"
     CDS             866..2938
                     /locus_tag="PKI07_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOC49461.1"
                     /translation="MLLEVLNQHGQFCSSLKLRISAGLWLLCATSVLAALPALVPRPQ
                     VLQTNAGVFTLCPPQAIPGLPTLAPTTIHADEVGRPSGEYLAMQLFKSTGYRFQVESN
                     SGAAAVSQAILLTTNGALGSLGGEGYELTIATNAIVIRAPAAAGLFYGAQTLLQALPP
                     EIYGARPVSGVPWTVPCLYIKDWPRFPWRGFMLDSVRHFFTKDEVKVVLDSMALHKLN
                     VLHWHLDDDSGWRLEIRKWPLLTQVGAWRTDMMWNLNPRASQCWDGTNYGGFYTQAEA
                     REIVEYAAQRHITVVPEIEMPGHSSAALAAYPQFACGCPTCYNGPYSLNVTSYVGGVF
                     CIARPETMDFLKDVLTEVMEVFPSPYIHIGGDEVRFTNWQNHNLDRALTNSLGIADMQ
                     KYQGHFTQQIANWIKSQGRTMIGWSEIMNGGLVTNAAVMDWLNTRAVQTATNRQYVVM
                     APSATLYINKWETGDQNGGGVVFSNEPPAQSGFVPLASVYAYEPIPTNLPPAYTNYIL
                     GAEGPCWSEWIPSLLNLQFRMFPRLSAIAEVNWTAPALKNWMDFTNRLVVHKQRLTHM
                     GINYNPSATPPQLGAWTPSLTPANYATLTWDITASVSAPGEINVSFCWKSGKHGLYIA
                     WAALLENEVEIDRDTHAGYTGYTPVKPSYVLRLPAYRPGATYRLAASVKGRDGTDSHG
                     IVYRTNWN"
     gene            2942..4216
                     /locus_tag="PKI07_00010"
     CDS             2942..4216
                     /locus_tag="PKI07_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOC49462.1"
                     /translation="MKPSWIKIVAAAALLLAGARAQALSIRTLAGGVTPGALNGFGSH
                     ARFNYPGGVAVDGAGNVYVADTLNSIIRRIAADGGVSTLAGLAGEYGSSDGAEAAARF
                     YGPQAIAADGAGQLYLADTVNSTIRKVTPAGEVSTLAGVPGDFNSFDGAGAAAQFHHP
                     EGVAVDAGGNLYVADTWNHTIRKITPAGLVSTLAGLAGYCGSADGTNSKARFNRPAGL
                     VVDGATNLFVADTFNHTLRQISPAGKVTTVAGLPGVWGSADGANSAARFYLPQGIAIT
                     TDGDLLVADSGNQTLRKVSPVGTNWVVSTVAGLPGLAGSVNGSDGHARFCFPAGLALD
                     NAGCLYVADAANHLIRTTRVVPPTLQFAALEGQMVLCWPTSAEGFVLEQSPTVDALAA
                     WSPATNGIAILGDSFICTNSPGAAAFYRLRFP"
     gene            complement(4238..7366)
                     /locus_tag="PKI07_00015"
     CDS             complement(4238..7366)
                     /locus_tag="PKI07_00015"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOC49463.1"
                     /translation="MKTPKPLFSSVCIAAAAFCALAWSLAAVAATGSSLAALEKQFRE
                     LPAEARRLTGPLFWLHGDESKERLELYVGKVAEGGNGCFTTESRPHNDWLGAGWWRDL
                     DICLRAAKQHNLQLWIFDEKWWPSQAVAGKVPPRYAAKRLAAAAVEVEGPRLFEAEGH
                     GGERYIATLAGRLDVEGKIDADSLVDLAPHIHAGTLRWPVPGGKWRILKFTHTQAPGL
                     LQVGGRQLTVDGASRDCVDWYLQTVYQPHFERYGADFGKTIRGFFYDEPETPGDWGTE
                     LNRVLAEWGVDWKLAYAAYKSELAGEAQIAARYQYLDALAEAWGRTMYGGIARWCRQH
                     GVKSTGHFMEHSRLYQHPDYCAGDMMRLQGHGDMGGIDAVFDQFVMGRREARDAPCWQ
                     TPKLASSISHAYGKPDDVAMVEIFGARGQTLTYPEMKWWTDHMQVSGVNFLIPHSFNP
                     RAPRDTDCPPYFYNGGFEPRWPLYRVFADYTSRLSLMLTGGRHVCPVALLYLGQSAHC
                     GQHILPDQISESLQDARYDCDWLPYAVFENDTRIRRATLQLRQERYRVLIVPPVEVIP
                     CATLAKAKQFFDRGGVVLGYGFLPAKSATPGKTAADITRLREALWGQPTPGLAVCKTS
                     PAGGRSYLLPAQPTPEQLQQVLAGDAGIRPTLEVLEGKTDHWLHVLHRVKAGRDVFFI
                     ANQNHEGAPRTFRCRITADGTPECWDAMRNEITALPYSHTGRQVELSLTLEPNESVLL
                     VFQPKPRALPPRPESGSAAPPRTIAVARDPAPAAPTPLPSLDNPAALALRGCPWVWYP
                     ETDAAHHAPPGPRYFRKQFNLPAGASIRKAVFVGTADNRFTLFINGKEAGQGDNSAEG
                     WRNPVELDVSRFLSSGPNQLAIVAVNAGDKPNPAGLLGRLTVELTSGSVLTERIDLSW
                     KVSREKAAQWTESGFDDRAWPAARELVPFGDGIWGMLGGRITVSPVQADPFLGRCELS
                     ASDLTSTRIYLELDRISPETAARVTVNGKDAGGFIGRPSRLEIGSHLHPGVNTFHIEP
                     FAPQSVRLAIYH"
     gene            complement(7552..9228)
                     /gene="rpsA"
                     /locus_tag="PKI07_00020"
     CDS             complement(7552..9228)
                     /gene="rpsA"
                     /locus_tag="PKI07_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOC49464.1"
                     /translation="MLTMEEALRQAGKPPFAAGNIVKGTIIEIGAKEALVDIGYKSEG
                     VIPASEFEDLKAVKVGDQIDVLIEKLEDKEGMVVLSKEKAEFKQNWERILTICNEGGT
                     IAGKVKAVVKGGLLVNIGVEAFLPSSHIDVVVSRNLAQYVGNTYQFKVVKINQERQNI
                     VLSRRELIEQERAERRQKLLTEMTPGDIRKGTVKNLTDFGAFIDLNGIDGLLHITDMS
                     WGRIGHPSEMLKVGQDIDVVVLDINRERERISLGLKQKLTNPWDTIEEKYPVGAKVKG
                     RVVSLVPYGAFVEVEPGVEGLVHITEFSWTKRVARPSDMLKTDQEIEAVVLGINREEQ
                     KISLGLRQLEANPWDQAQEKYPAGTRVKGKIRNLTSYGAFIELEEGIDGMIHVSDMSW
                     TRKINHPSEMVKKGDEVEAVVLEVDKANQRIAVGMKQLAADPWEGIDQIYKVGDLVTG
                     DVTKLASFGAFVGLQHDIDGLVHISQISEERIDKIKNVLKVGQEITARVIKIDRSDRR
                     IGLSIKAANYSTEQLKAEQAILDALKPGEDLVALQHAFDAADEAKQEESQ"
     gene            9519..11471
                     /locus_tag="PKI07_00025"
     CDS             9519..11471
                     /locus_tag="PKI07_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOC49465.1"
                     /translation="MHVQLAWPDYFILLAYTGFVIGIGFALRKYLKSSSDFLTSGRSI
                     PTWVTGLAFISANLGALELVGMAASGAKYGIATSHFYWVGAIPAMVFLAIFMMPFYYG
                     SKARSVPEYLKMRFDERVRALNSIAFAVMTVFASGISMNALAKLLNQLLGWNYHVALW
                     ICSGVVLVYVLKGGLTSAIYTEVLQFFMIVLGFAPVVYLGLKDVGGWGAMNQALQGVA
                     VDPAALHLKEAGTTYAGNAWSSAWQPLLAGPAHNPMGVDWFAMVFGLGFVLSFGYWCT
                     NFLVVQRAMAARNMTAARNTPLVAAVPKMLFPALVIVPGMIAVALTSMPGKNYRIPPP
                     IIGDEVYTRAAEVVKTVGALEAPAAFEAVREAMGKKMDPVKVAALIRDAGHLSEGQIK
                     DGLYEAVAEYDYDGVILSLVKKYCPTGLLGLALTALLASFMSGMAGNVTAFNTVWTYD
                     LYQAYIAPKKSDQHYFWMGQAATVVGILVSIGAAYFASMYNNAMDIIQLVFGFVNAPL
                     FATFLLGMFWARCTGTGAFLGLFGGIGGSALFHACTTAVSNLDASGRVVNGMKGGYLG
                     VVQSFPSEMAQNFWLAGFAFVVCFTLTLVISLVTRRTKTNEELKGLVYSLTPKIKDEG
                     APWYLRPAVVGTALIVGCVILNIIFW"
     gene            11488..11712
                     /locus_tag="PKI07_00030"
     CDS             11488..11712
                     /locus_tag="PKI07_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49466.1"
                     /translation="MGLDIRWPIGLMFSLVGLLLTLFGLCTRGDAEMYQRSLDININL
                     IWGIILLLFGAFMIIMALRKKPDPDALKSK"
     gene            11868..12917
                     /locus_tag="PKI07_00035"
     CDS             11868..12917
                     /locus_tag="PKI07_00035"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOC49467.1"
                     /translation="MKATVVRTAFGKLPSGRAVDLFTLTNTRGLVAKITNYGTILTEL
                     HVPDRHGRRADVVCGFDHLESYLKGHPYFGCTVGRVANRIAGGKFMLDGRAYSLAVNN
                     GPNALHGGLRGFDKVLWKATPLREATVRFDYTSADGEEGYPGRLAVRVIMKLTEDNEF
                     LIDYTATTTRPTPLNLTNHSYFNLAGRGDIKAHQLMIAADFYTPKSPADVPTGEIRPV
                     KGTPLDFTRLTAIGARFARLGGRPQGYDHNFVLRGDPANSGQPAFGARVVEPRSGRVM
                     EVFTTEPGLQFYTANWLDGSLIGKGGFSYSRHCGLCLEAQHFPNAINIPHFPSTVLRP
                     GQTYHQLTLHRFSTA"
     gene            12945..14114
                     /gene="galK"
                     /locus_tag="PKI07_00040"
     CDS             12945..14114
                     /gene="galK"
                     /locus_tag="PKI07_00040"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOC49468.1"
                     /translation="MTLNELARQIALDFKQTCNREPQWIVAAPGRVNVIGEHTDYNDG
                     FVLPMAIDRYTLIAAAPNGTTQITLRSSAGQGQARVDLTSPLQPGTKGDWANYPLGVI
                     AGFLARGLRPPGFDALIHSTVPLGGGLSSSAALEVATATLLEVITGQKLDPVEKALLC
                     QKAEHDYAGVPCGIMDQFISVMGKENHLLLLDCRSRRPELVPMSDSSVALLIVNTNVK
                     HELTGGGYAQRRAQCEQAAQILGVPSLRDATEDLLQAARSRMDDVVFRRARHVIGEIQ
                     RTVQAAAEVRASNWPVVGQLMYASHASLRDDYEVSCAELDVVVDIGRAIGPAGGVMGC
                     RMTGGGFGGCAVALVRADQMAAISQRLAAEYEQRTNIKPTLFVSRPAAGAMVLKP"
     gene            complement(14076..15236)
                     /locus_tag="PKI07_00045"
     CDS             complement(14076..15236)
                     /locus_tag="PKI07_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOC49469.1"
                     /translation="MNAKDRMTPAENPASTAESAPRILEHLVAQAEQAGASDIHLQML
                     SDGAAVTFRLDGVMTPTDRLPAAIAERVFGRVKFLARLKTYQESLPQDGRIDRADLNA
                     RADIRVATYPTVSGEKIVLRLFDAPGARRLEELGFPAPALAELEGFLRQTAGLLLLTG
                     PAGSGKTTTIYACLRHLAEQGGRHVITVEDPAEQVIPGIMQTEVNEARGLDFARAARH
                     LLRQDPQVLVIGEIRDEETANLVVRAALTGHLVISTLHAGSCPGVFERLLVLCPDHSA
                     VASSLELVLNQRLLRRRCERCAGKGCPACLDTGYRGRLPLVEWLRLTPALRRRIATRD
                     LDGLVAHPALAESAQALVQAGLTDAAEVARGLGWTGVAPGSGLQNHCPSGGA"
     gene            complement(15245..16006)
                     /locus_tag="PKI07_00050"
     CDS             complement(15245..16006)
                     /locus_tag="PKI07_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOC49470.1"
                     /translation="MPKETWMIAAGLTVALLLGLLGLASWEKKREVEKVRAEMAAARA
                     DAARLRAVAEGLKDQLAQSQARIGELQAEKQTVLHTHQTLEDEMRAALESKDVTISQL
                     QGKLTVNILDRVMFDSGEADLKSAGSAVLRKVAGILAAHPNLKIHVIGHTDNVPIRAS
                     ARSRFPSNWELSTARATAAVRFLTETAGVDPRRLGAVGYGEFRPVADNSTPEGRALNR
                     RIAITILSEEMAGADIAAPPASAAGTNAPVPAPET"
     gene            complement(16141..16497)
                     /locus_tag="PKI07_00055"
     CDS             complement(16141..16497)
                     /locus_tag="PKI07_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49471.1"
                     /translation="MNLNHSAFPKLLAKRPVQITNSTFNVKWFLRQAGPSVCEQPGLL
                     GTLDNFSARSFKEKPPLEKTVPGSTSRTLEFHLTPSVNAANEPNLRPVPPNRTSGPVP
                     CAPVNVSSPLFPPKAV"
     gene            16483..17436
                     /locus_tag="PKI07_00060"
     CDS             16483..17436
                     /locus_tag="PKI07_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC49472.1"
                     /translation="MIKVQNLAKLFGTKRAVDDVSFSVNRGEVLGFLGPNGAGKSTTM
                     RVITGFLPPSAGSVTVGGFDVVEHAIQARRLIGYLPENAPAYTDMTVLGFLNFAAEIR
                     GLRGEARKQAVGRAVEMCFLESVLHQSVETLSKGYRHRTCFAQSIIHDPDVLVLDEPT
                     DGLDPNQKHEVRQLIRRMGEKKAIIFSTHILEEVDAACTRAIIIDRGKIVANGTPQEL
                     RQKSEWAGAVTLRVRNVAAGTVSTRLYQVPQVKRVTVVEDKDGHVTVRAFPRSGANGA
                     LVAAVGEAAREWQIEQLQAEEGRLDEVFRSITMPDTAKEAA"
     gene            17436..18179
                     /locus_tag="PKI07_00065"
     CDS             17436..18179
                     /locus_tag="PKI07_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC49473.1"
                     /translation="MNAWHNVWTITKRELGAYFTAPLAYVFIVIFLLLCGFFTFFVGG
                     FFERQEASLVRPFFDWHPWFYLFLVPAVGMRLWAEERRVGTIELLLTMPITAWQAIVG
                     KFLASWLFLALALALTFPVVLTVNYLGDPDNGVILTAYIGSWLMAGAYLAISCITSAL
                     TRSQVVSFIISLVVCLFLILAGFPPVINVLEQVAPRLVDLVTSFSVMTHFEGFQKGVL
                     DSRDLLFFLSVIGFSLFTTSVILRTHRAG"
     gene            18193..20112
                     /locus_tag="PKI07_00070"
     CDS             18193..20112
                     /locus_tag="PKI07_00070"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOC49474.1"
                     /translation="MQKKSFQTILYSTVGVLVMLVILVAFNFIAGALRMRLDLTQEKA
                     YTLSDGTRAILKKLDTPVTIRFYCTQSESATPETVFLKSFARKVEDLLTEYRQVAGGK
                     IKIEKYDPQPDSDAEDSARLDGIEPQALAGVDRFYLGLAVKCLDEVQSIPFLAPNRER
                     LLEYDLARIIVRATNPEKPVIGVMSPLPVFGMPSNPMMQQMGQQGSQPWTIISELKND
                     FSVRRIGMDADKIDDDVKLLVVIAPKDITDKAQYAIDQFIMRGGKLLAFLDAQCLADS
                     RQQNQMMMNMGGGGSSLDKLLKAWGLQFDSSKAVADLKYKMQLRGRNGEPQEAPAWLG
                     LTADAIDRDDVATSQIDNVWLPLCGAFTGTPQDGLKQTVLLHSSKDSQLVDAMLANLS
                     GESTMKEFKPSGVNYNLAIRLTGKFKTAFPDGAPQDAKSEDEKKDEAKPDESKAGDSL
                     KETRSDNTVVLFGDADLLFDPFTIRRIDSPFGALQMPMNANLNLAQNIIEQMTGDNNL
                     IAVRSRATLSRPFTRVKEIEAAANKKFQSEIKRLEDSAAEAQRKVNELQAQKQDKDQR
                     FILSPEQRHELEKLRKEEVDTRKRLKQVQKDLRKEVVSLQTRLKWINILAVPLAVTAT
                     GVVIAMVNRRKTSAK"
     gene            20109..21347
                     /locus_tag="PKI07_00075"
     CDS             20109..21347
                     /locus_tag="PKI07_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOC49475.1"
                     /translation="MNRKQLLILLVLVVVVGAAGLVVYQRNQSSWQGGGREGDSRKLL
                     GELPVNDVAAILIQRGTNQLDLVRIDNRWRVKQRDDYPANFSEISSFLLKAADLKAAQ
                     TEEIGPSQLGRYQLLPPGPETNTAVRVELRGQDGKPIKSLLLGKSHMRKAAGRASPMG
                     EMEEDEGWPDGRYVMVGGQVKTVALVSDPLSNLEPKPEQWLNKDFFKVEKIRSIAVTY
                     PAATNSWKASRETESASEWKLAEAKPAEQLDPAKTSGFAYALSSPSFNDVLPADTKPE
                     QVGLNHPTTLTLETFDDFTYSLKIGQRTNDNLPVVLAVTAQIPKERTPGKEEKDEDKA
                     RLDKEFKEKQKKLEDKLTQEQGYGKRIYLVSSWTLDALLKERHQLLLEKKEEPAGEEK
                     PAAPPTKQAEDLMEPAVEPK"
     gene            21624..22436
                     /locus_tag="PKI07_00080"
     CDS             21624..22436
                     /locus_tag="PKI07_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49476.1"
                     /translation="MKAFSASGWATKALAGLMLAAYAVLPSSAVGATESKLEVLQTKT
                     ATYTNVTVTTKAKGYIFIVHDRGMASLKVADLSPEVQQQLGYSVAGAAKHSTNTAVAW
                     TKREVARLAGPEVKALETRLQQKWSGHPPAEAVLRGMIGPQQVLAFLGVLLAFHLFYS
                     YCCLLICRKTGNPGGFWVWVPLFQFLPLLRAAGMSRWWFLACFVPLLNLVPAILWPLK
                     IATARGKSVWIGVLLLLPVTNVFAFLYLAFSNGAPARDEKRPAAKIMTLQAA"
     gene            complement(22521..22973)
                     /gene="dtd"
                     /locus_tag="PKI07_00085"
     CDS             complement(22521..22973)
                     /gene="dtd"
                     /locus_tag="PKI07_00085"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOC49477.1"
                     /translation="MRAVIQRVSEASVRIAGVTKGAIAQGLLVLLAVEEADTPADLEW
                     LSGKIVRLRVFDDENGVMNRSVQEIQGGLLVVSQFTLFASTKKGNRPSYSRSARPEIA
                     VPLYESFLQRLALDLGRPVQSGEFGADMQVSLTNDGPVTIIIDSKARE"
     gene            complement(23028..23336)
                     /locus_tag="PKI07_00090"
     CDS             complement(23028..23336)
                     /locus_tag="PKI07_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49478.1"
                     /translation="MKVFVRDTQSGWFYQGPSQWTPEQKMALDLGQVARAVEQIFEAR
                     LENVEILLSYDEPRYDLVLPVPAAPSRADTSRRPDPEQPDMPGERKSAAHRHKKAPPL
                     "
     gene            complement(23487..23846)
                     /locus_tag="PKI07_00095"
     CDS             complement(23487..23846)
                     /locus_tag="PKI07_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49479.1"
                     /translation="MNDREAVKVLIVNDDGQYLTGTAFEWEFTDERGKARVFDYQADR
                     VGELIDLVRKTQGRVWIAVKLDPREVFEFCDRCGCRMWAFKTYFDGRQFLCRNCREVE
                     SGAKPGLPPRGASGEVP"
     gene            23960..25453
                     /locus_tag="PKI07_00100"
     CDS             23960..25453
                     /locus_tag="PKI07_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOC49480.1"
                     /translation="MSEAAKLIRIPSLKTVAEFRGHVASLGIDLPCEDAIATGDASPL
                     AQPCRAVLINGKRLGNRYAVQPMEGWDATVTGGVTPEVLRRWQRFGQSGAKLIFGGEA
                     MAVRPDGRANPNQLIINARNQSDLAKLREALIRAHQELYGRSEDLVIGFQLTHSGRFC
                     KPNDHSRLEPRVACRHPLLDASFKVTSDAQVWTDSELDQLVEDYAAAARIAGNVGADF
                     VDIKHCHGYLLHELLGAFTRPGKYGGSFENRTRMLRDIVAAIRAGGNRIELAVRLSAF
                     DFVPFRPDPALSRPGRPGPGVPVPFSHCLPYRYAFGVNPQNPITYDLTETFQFLDLCA
                     SLGVKIINLTAGSPYYNPHIQRPAAYPPSDGYQPPEDPLVGVARQINVVRQLKARAPQ
                     SLLLVGTAYSYLQEYLPHVAQYVLRHDWADLIGIGRMVLSYPTILADAIGRGTLQSKA
                     ICRTFSDCTTGPRNGLISGCYPLDRYYAVRPEAQKLKEIKKASEQAA"
     gene            26141..27733
                     /locus_tag="PKI07_00105"
     CDS             26141..27733
                     /locus_tag="PKI07_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC49481.1"
                     /translation="MQSALPEATAAPLYFNFASDVIDRWARERPDAPALWCVDAATGE
                     GRKFSFRQLAELSCRAANCLRANGVGRGDRVLLMLPRVPQWWIAMLGLIRLGAVPVPA
                     TLLLTARDVAYRLGSARIRAAITSLDGVGKVGGFEGVRLLVGGQRAGWADFDDGVRQA
                     SPEFRAEPTRSDDPGIIYFTSATTGDPKMVLHTQASYGLGHRATGELWLDLKPDDLHW
                     NLSDLGWGKAAWSSFYGPWHMGACVFALDTPGKFDPVLTLDTLAAFPITTWCAPPTAL
                     RLIVRQDLSRWRFPHLRHCVTAGEPLNPEVLNRWREATGLTLYEAYGQTETVVLIGNF
                     RSLGHPVRPGSMGKATPGYSVALLDEHLREVPDGKEGEVAVRVKPHRPAGLFSAYWLS
                     PREMAAQFQGDWYLTGDRATRDADGYFWFIGRKDDVIKSSGYRIGPFEVESALLEHPA
                     VLEAAVVGQPDALRGQIVKAYVVLQPGMEGSPEVKAELQTHCKSLVAAYKYPREIVFV
                     RELPKTTSGKTRRVELRQITPV"
     gene            27786..28484
                     /locus_tag="PKI07_00110"
     CDS             27786..28484
                     /locus_tag="PKI07_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOC49482.1"
                     /translation="MKSLTECRLYAFVDTAFLQGRAPGEVARALCDGGADLIQLRAKE
                     APEAEVWRLAEAILPVTQRAGIGLVINDYPAIARELGAEFCHLGQEDFFGAGYTQVSQ
                     LPLAAEPEKPPAGEGGGCRPRVRVGLSSASPDQARRAIAAGADYLGVGPVFPTGTKPT
                     ARAVTLEYVRWAAANIPAPWFAIGGINLENVDEVLAAGARRICVVSAILAAPDPAAVC
                     RKFQERLGRAARQG"
     gene            complement(28498..29355)
                     /locus_tag="PKI07_00115"
     CDS             complement(28498..29355)
                     /locus_tag="PKI07_00115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC49483.1"
                     /translation="MFNKANRSEVHATPAPGPFGRFYLQELINSGGMADIWLATDERQ
                     KPYALRRMHARLRFDFSARRRFLRGAEILSKIGDHAGIVGYVEHGKIKGQPYLLMDYV
                     EASNLKQLHADQDPVLVENVAQILIDMAAGLEHMHDNGFMHLDFKPENVLVTRNASVR
                     LVDFDLAQPIPEKPRKPSGKNPGTPAYMAPEQLRGEPISPRVDIFSYGVAAYELLTNQ
                     KPFPGETPGEILARQLDRSGFVAPRQHNPSIPIAMEKVILRCLEGDPERRYPFMGMMA
                     REIQAALYV"
     gene            29444..30196
                     /locus_tag="PKI07_00120"
     CDS             29444..30196
                     /locus_tag="PKI07_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOC49484.1"
                     /translation="MQGLRGFTDAVRGWLAAGLALVYPEVCQVCGEARAGPGDGYVCG
                     GCQARVRFIEPPFCDRCGRPFEGDLTTRFECASCREMEWHFHSARSAVATRAPVLEII
                     RRYKYNRALWFEPFLAGLLIRAAGPVLSGQAPDLIIPVPLHFTKKREREFNQAESLAE
                     RLGAAAQIPVNKRLLRRVLPTRSQTRLSRQERLVNMRGAFAVQAGAKLNGERVVLVDD
                     VFTTGATTNACARALITAGAGEVSVWTVARGI"
     gene            30309..31268
                     /gene="accD"
                     /locus_tag="PKI07_00125"
     CDS             30309..31268
                     /gene="accD"
                     /locus_tag="PKI07_00125"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOC49485.1"
                     /translation="MATTSFTKKTLGAEAPEPAVTPPSPSNGTAFVKKPKLPSRKSRK
                     RDIPEGLWTKCPKCGTMVFDKELDENLKVCLHCQHHFPIGARERIHSLVETCTFEEMD
                     AAMTSVDVLSFTGVTSYTSKLASYQKATGLKDAVLTGICKMGRHRVALGVMDFSFLGG
                     SMGSVVGEKLTRLIERATEKTLPLIIISTSGGARMYEGMFSLMQMAKTCAALAYHAKA
                     RLPYISVLTHPTTAGVMASYASVGDLIIAEPAAMIGFAGPRVIKDTTQAELPPGFQTA
                     EFLLDHGLIDAIVPRTEMKPRLIEYLDYLTEGQGNDASSKSAA"
     gene            31369..32286
                     /gene="rfbA"
                     /locus_tag="PKI07_00130"
     CDS             31369..32286
                     /gene="rfbA"
                     /locus_tag="PKI07_00130"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOC49486.1"
                     /translation="MKGIILAGGAGSRLFPLTLVASKQLQPVYDKPMVYYPLTTLIES
                     GIREFCLISTPQDLPRFRQLLGDGSAWGLSIEYREQPRPQGIAQAFLIADTFIGSDAV
                     TLILGDNVFYGGDGFSRAFAEFKGGATIFGYHVNDPERYGVVEFDGNGQAISIQEKPR
                     QPRSNYAVPGLYVYDNNVVAITRGLKPSARGELEITDVNVEYLRRGQLRVYRLGRGFA
                     WLDAGTSSSLHEASAYVQTIEKRAGIKIGCPEEAAFHRGFLSLARLEALTAKMPNCEY
                     RDYLAEVVAEAKRLGAVEAQGPAAGVETR"
     gene            32283..32831
                     /gene="rfbC"
                     /locus_tag="PKI07_00135"
     CDS             32283..32831
                     /gene="rfbC"
                     /locus_tag="PKI07_00135"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOC49487.1"
                     /translation="MNVLKGEIAGLLIIEPKVHGDARGFFLETWNRGRYREAGLDVDF
                     VQDNVSFSRRGTLRGLHFQNPNPQGKLLQVLQGEVFDVAVDLRRSSSTFGKWQGLLLS
                     GENKRQFYVPPGFAHGFLVLSETALFHYKCTGFYLPKDEISVWWNDPDIGIKWPLQEP
                     LVSERDAKGLRFRDLPPDRLFA"
     gene            32884..33768
                     /gene="rfbD"
                     /locus_tag="PKI07_00140"
     CDS             32884..33768
                     /gene="rfbD"
                     /locus_tag="PKI07_00140"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOC49488.1"
                     /translation="MNPPHIMLIGKVGQVGWELRRALAPMARITCVDFPDIDLTSGDS
                     IRQWVRGSRPDIVINAAAYTAVDKAESEPDKAMAINGVAPGILAEEAKRLNALIVHYS
                     TDYVFDGSSTQPYVETDAPAPLGAYGRSKLAGDQAVQAIGGAYLIFRLCWVYGARGQN
                     FMLTMMRLAREREKLRVVSDQVGCPTWSRVIAEATALALKAATAAPDRQAFSGIYHLA
                     AGGMTSWHGFAQAIINLMPEAEKKCAEIEAISTAEYPTPARRPAYSVLACDKLERTFG
                     LRLPHWEESLRQVLEVGH"
     gene            33790..34800
                     /gene="rfbB"
                     /locus_tag="PKI07_00145"
     CDS             33790..34800
                     /gene="rfbB"
                     /locus_tag="PKI07_00145"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOC49489.1"
                     /translation="MNLLITGGAGFIGSNLIHQVIDRPEVARLINLDCLTYAGRLENL
                     VAVAGHPKYAFEKVDLRDKAAVLEVVQRHAVTHVMHLAAESHVDRSITGPGDFVHTNV
                     VGTFNLLEACRAAWAGAQGSRRFHHISTDEVYGSLGPTGFFTEATPYAPNSPYSASKA
                     ASDMLVRAYHHTYGLETVITNCSNNYGPYQFPEKLIPVVIQSVLARKPVPVYGDGMNV
                     RDWLYVCDHAEALWTVLTRGSSGETYNIGGHNEWANLNIVQLICDLIDELAPQLGGGS
                     RKLITFVKDRPGHDRRYAIDATKIEKELGWVPAHRFEDGIRATVRWYLDHQEWVQAVL
                     KH"
     gene            34842..35837
                     /locus_tag="PKI07_00150"
     CDS             34842..35837
                     /locus_tag="PKI07_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC49490.1"
                     /translation="MQQKVLLLGGTGNISTACAAQLRQQGHEIYILSRGQTTVPPGYQ
                     PLRADRKDPAAMRHVLKSVRPDVVINFIGYDLPEVQIDCELFSGSVSQYVFISSAVVY
                     AKPPARLPLTEDAPLGNPWWDYAQKKLACEQWLLQRRQETGYPVTIVRPSHTYSKLWV
                     PNAVSSGSYTLAARLERGKAVFVHDDGESPWTLTAASDFAIGLAGLVGKPAAIGEAFH
                     ITSDEVLSWNQIYAEIAAAVGAEAPRVVKVPTDFICQVAPQMTGPLKGDKAHPGIFDN
                     TKIKRLVPEFRCRKSFHTGVRESVQWLRAHPEQQNLRPELDALIENVITAWERQG"
     gene            36507..37256
                     /locus_tag="PKI07_00155"
     CDS             36507..37256
                     /locus_tag="PKI07_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49491.1"
                     /translation="MTILDCNGRLISEIACAAPVRCVAVAAEGTVYVGVKDHVETFDR
                     TGQRLATWPSSGRRTWLTGLAAGEKDVFAADSGNRCVLRYDKAGKLLGRIAERNKDRH
                     LPGLVVPSPYLDVKLAPDGLLRVNNTGRHRVEAYTVDGDLEFFWGRPSAGIEGFCGCC
                     NPVGLALLPDGRHVTCEKGLPRVKVYSPKGVFESVVAGTETFYENAKTGALKTRSDGL
                     VGGLDAAVDSQQRIYILDLVANDVRIMQRKA"
     gene            37253..37825
                     /locus_tag="PKI07_00160"
     CDS             37253..37825
                     /locus_tag="PKI07_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOC49492.1"
                     /translation="MNPGISKPSSVTRRQFLRDGARAAGLAGLGGLLGLVAERSTAKG
                     TVWQIDPQKCVRCGNCALYCVLEQSAVKCVHAYALCGYCQLCTGYFEPDPNDLNTGAE
                     NCLCPTSAIKRTYIEDPYYEYTIDEQLCIGCAKCVEGCHRFGNGSLFLQVRHDRCLNC
                     NECAIAKVCAGDAFRRVPADQPYLLKTDTF"
     gene            37896..39218
                     /locus_tag="PKI07_00165"
     CDS             37896..39218
                     /locus_tag="PKI07_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC49493.1"
                     /translation="MALLWLLSDVASLAEQRFPPPDFEGGHQLPVTTTPPARALTLDY
                     LDVGVLGLSLGLAIWLVYRRRSRKGLIVLSIFSLLYFGFWRHGCVCAIGSLQNVALAL
                     GDRSYALPLAALAFFVLPLAVAIFAGRIFCAGVCPHGALQDLVLLKPLKVPPGLEAAL
                     SVLPFLYLGAGVLFAATGSAFIICEYDPFVPVFRLSGRALMVLSGAALLGLSIFVGRP
                     YCRFLCPYGALLKLAATVAKWRVRVTPDNCTQCRLCEASCPFGAMREPQARPGTQTHV
                     RDRRRLGWLLLLLPLLLVGGGWLGHRFSTPASRLHPTVSLADLLIRAQGSPPKIGALT
                     PDDLALERARQAPQEILAAAAAIQRRFAIGGWIFGAWVGLVFGVKLISVSVHRPRTDY
                     EPDSGDCFACARCFEYCPNELVRRGVIPATTLTAAAPEISGPPGLDRS"
     assembly_gap    39371..39470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <39471..40862
                     /locus_tag="PKI07_00170"
     CDS             <39471..40862
                     /locus_tag="PKI07_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOC49494.1"
                     /translation="ARARWAVAACGIVVGSGLFVLSRALGPNLPTPAAELDKLLATRP
                     EESSSPGHPQAADAASLAELRQNWPRFLGADGNSFSPSTNAPASWNPQTGAGIAWKVP
                     TPTRGFGSPIVWAGQVFLSGGDARQREVICLDAQTGKILWQQPVASVSDRPDRTEVPD
                     STGYAAPTMATDGRRVYAFFANGDCAAFTLAGQPAWARNFGPLKNPYGHATSLATWRD
                     RLILQLDQGESEDGNSKLYALDARTGQTVWQRPRKTGATWATPNVIDAEGKAQVIALS
                     VPWVIAYAAADGAEVWRAECLNGEIAPSPACAGGLLFVASPSEKLLAIRPSGQGDITQ
                     THLLWTWEDSIPDVTSPASDGELVFILTTSGMLTCLDARDGKKQWEHDLEMECHASPA
                     LAAGRLYLFGQKGTAVVVAAAREFRELFRTEMGDAFHASPAFVQDRMFLRGMTNVWCV
                     RTINEAGKEGAQP"
     gene            40859..43291
                     /locus_tag="PKI07_00175"
     CDS             40859..43291
                     /locus_tag="PKI07_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOC49495.1"
                     /translation="MNPARTEPGVGPVDLQYVDAAIQRIGRAPDAVIPLLQAIQDHYG
                     YLPEAALRRVCAATQITPAGINGVATFYDMFRHRPGGKHIVQVCHGTACHVGGSERVE
                     EALRRHLAIPEGSDTDAARRFTLERVACLGCCTLAPVVRIEKETIGYAAAEKVPETIA
                     GFLANRQEAAATPTVATPAPRTGAAEIRVCLDSCCLAKGTDHSFRALQQSLAKHGANA
                     TLKRVGCVGACYRTPMVEIAIPGQPSIIYEGVTPEQAGRLVETHFRPAGLTPRISQLY
                     TRIVDGLLVEDADAIPPPGRPADAKDGAGDQAFFRRQVHIAMEHYGRLDPLDLDEYLA
                     HDGFAALRKCLGIPPPEENRRVDGGPNSAAVSPEQIITSLERSGLRGRGGAGFPTGQK
                     WRMVRQQPGATKYVICNGDEGDPGAFMDRMILESFSYRVIEGLAIAAVAIGAHEAIFY
                     IRHEYSQALQRVRAALAECERRGWIGPRLLGSDYPLRITIKEGAGAFVCGEETALIAS
                     VEGRRGMPRLRPPFPAQAGLWGKPTLINNVETLAMVPWIIRRGAEAFAAIGTAASKGT
                     KVFSLAGKVRRAGLIEIPMGTTLRQIVEEIGGGIADGRTFKGVQIGGPSGGCVPARLA
                     DTPVDYESLRDIGAIMGSGGMVVLDDTACMVDLARYFLQFTQNQSCGKCTFCRIGTKR
                     MLELLELFCAGQATRKHLRELEQLAPQVSAGSLCGLGKTAPNPVLTTLRYFRDEYEAH
                     LQGRCPAGRCKALIKYQVGDNCTGCTLCAQHCPADAIPMTPYERHVIDLQKCTRCDSC
                     RQVCPYGAVEIV"
     gene            43288..45360
                     /locus_tag="PKI07_00180"
     CDS             43288..45360
                     /locus_tag="PKI07_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC49496.1"
                     /translation="MSAIAIHIDGRPVSVPPGTTILGAARKLGIAIPTLCHVEGFEPS
                     ASCFLCAVQIEGRPNLWPSCATPVAPGMAVITNSAEVRAARKTALELLLSDHAGDCVA
                     PCHTGCPAGLDIPGFIARIAAGDNAASARLVTDGLTLPASLGRVCPRLCEQHCRQCDV
                     TEALSIRNLHRFSADYQRQAKSPPPPADPPAPPTGRRVAIVGAGPAGLAAAHHLLGQG
                     HAVALFDAHSQPGGMLRYGIPAFRLPRQVLDAEIQAIRALGAEFHLGRRLGRDLALDN
                     LRRDFDAVFLAIGAQGSRSLGCPGEELATSALEFLETIADGSPPAIDGDVIVVGGGNT
                     AMDASRCAVRLGARSVRVLYRRTRREMPCLMEEVEAAEAEGVQVEFLVAPARLERKEG
                     KLNLICQRMELGPPDASGRARPVPVPGSEFALPATCVIAAIGQHVKTDGLDHSQLALS
                     KWGIAADPKTLATNLPGVFAGGDAVSGPDLAVRAVAAGKLAAVAIHQYLAGQKVVGTP
                     PLASVMMGKLSEAELAVLLRDIEQAPRAPMPHLPLEARRTTFDEVELGFSLETATRES
                     RRCLGCGCGKAVPCRLRQVATEYGVDPQRFVGERRLFTRDTSHPDIIFEPGKCILCGA
                     CLQVAAQAGEELGLSFAGRGFQVSVAPPFHRPVAEALRQSGRRAAEVCPTGAIMLKSA
                     GCAGCRLA"
     gene            45827..48490
                     /locus_tag="PKI07_00185"
     CDS             45827..48490
                     /locus_tag="PKI07_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOC49497.1"
                     /translation="MSRKASASSDSSRGFSDIIGIVLMCLAILLMVALLSYHPRDVSA
                     NAVPHNQPALNWIGPFGAWMAYGCFLTLGAAAFVLPVVLFFVGVGCFFEMFAYLRRRW
                     VWAVVLLICCMGLLDIYRHYLGGLERNLGVVAGGILGRNLNQHLFSECFGAVGATIIF
                     LMLYFISLLFLTNFQLGDWVRALWSRRPRAGGKAVTEEQALEQRARELQQQARKLQEE
                     VDRAGLGADMQPVPKPTVRDLSVPEANKAARGRKAAAASPAPEPAPADEGEVIPAHEV
                     AAATTAEVLGQPPEPVADSAEARAAGDKAETAAAEKSEGADKPSEPRRDQEVVIHGLP
                     SAASARPRKKPKPIAVAAAPVIGNYQLPPLDFLQHPDPNLKPTESKEELLANARLMQQ
                     TLAQFEIPVELGDITKGPTITRYELHPAPGVKLEKITALNNNLAAALKAERIHILAPV
                     PGKSSVGIEVPNPVKTAVIIRDLLESEEWRNTKARIPLALGKDVYGHPIISDLSEMPH
                     LLIAGSTGAGKSVCINAIITSLLYRFPPDRLRFVMIDPKVVELQQYNLLPHLVVPVVT
                     DPKKVILALRWVVNEMEKRYQIFARVGVRNILSFNSRPRNKPLPPREPELELLARKEK
                     VEPGADGFAVEVDEAIVVPREEDIVIPEKLSYIVVIIDELADLMLVAPADVEMAIARI
                     TQMARAAGIHCIVATQRPSVDVITGVIKANIPARIAFQVAAKVDSRTILDAMGADKLL
                     GKGDMLFLPPGSAKLIRAQGSLITDQEIQNIVEFIAKQGKPSYELEIHQQLSRADNGS
                     GSGSGIDEDEEIIQDCIEVIRSEQKASVSLLQRRLRLGYGRAARIMDELENRGIVGPS
                     KGAEPRDILIDLDGGGADGRPPQNEEPPPPA"
     gene            complement(48517..49407)
                     /locus_tag="PKI07_00190"
     CDS             complement(48517..49407)
                     /locus_tag="PKI07_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOC49498.1"
                     /translation="MTNSPDDPPRLRMAPVETPAEAAHRFEREQRAVDLQRERSLEQS
                     LAPFRVGNSGFLNTVPLTRGLEEEIIYAPPARLAEMLQRDELDAALVSVVEVLFHDRY
                     DILDGIGIAALGEVKSVLLAHRKPLDEAAEIFCDPASLTSVQLLRILLAERGITPEFK
                     PLPGYDLAALPDYALLIGDTALDLALGPHQHDIWDLGAAWYEMTGLPFVFAVWALRRG
                     VENARLRRLLREAHDFGMDTLDSIIRTRTEYTSDFRKDYLGWHIHYHIGADEKRGLAK
                     FIDLLRRHRAGPVHEPRFIV"
     gene            complement(49404..50570)
                     /gene="mqnE"
                     /locus_tag="PKI07_00195"
     CDS             complement(49404..50570)
                     /gene="mqnE"
                     /locus_tag="PKI07_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOC49499.1"
                     /translation="MHFFVQRSELRDLYDKVAAGQRISEADALRLFESKDLNALGAIA
                     SLARQRKVGRRASYILNRYINYSNYCILSCQFCSFARKKRDANGFQLSLEEIVAKARE
                     ALQFGITELHIVGGLHPSLPFSYYTDMLRALRALDARLQLKCFTAIEVLHLAWLARKP
                     VAETLAALKEAGLDSLTGGGAEIFRPEVRSAIARGKESAEEYLEVHRTWHRLGGRSTC
                     TMLYGHVESLADRVDHLRQLRGLQEETHGFTGFVPLPYHPENNGIPVANPPTGFDTLR
                     TIAVSRIYLDNFDHITAYWVGLGLKLAQVALGYGADDLHGTIFEEHIFHQAGAQTPQL
                     QSETAMIQAIREAGCIPVQRDTFYEPVRVWEDTPPEADPGNAPATVREANLASA"
     gene            complement(50612..51916)
                     /locus_tag="PKI07_00200"
     CDS             complement(50612..51916)
                     /locus_tag="PKI07_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOC49500.1"
                     /translation="MTAFTHRFAPLALAAACGCASHSLPPRAFPDAARLPAQATLPDP
                     LIKMDGHRIKSRSEWFRQRRPELKELFAYYMYGPIPPKPAQLKATVTGEHRDFLGGQA
                     TLRLITLEVAPHDPQAPRIDLMMILPNHRPGPVPVFLALNFWGNQAISSDPRIPLTRG
                     WLRGNCRGCTNNVATDAARGTQSDDWPLAEIVSRGYALATFCNADLDADRPDASLGLY
                     AWLAGNDPRRNTPADRGTIAAWAWGFHRCVDYLVTDRDIDAKRIATLGHSRNGKTALL
                     AAAFDERIAMAFPHQAGCGGTAPSRGKIGESVKAINTRFPHWFNARFKQFNDAPERLP
                     FDQNALVALCAPRPVLFSAAEEDQWANPAGQFEVLQAADPVYRFLGSPGLAATEMPPP
                     GKLIASPLGYYIRAGKHSMTAADWRVFLDFADQHFRRQPKLP"
     gene            52030..53043
                     /gene="glpQ"
                     /locus_tag="PKI07_00205"
     CDS             52030..53043
                     /gene="glpQ"
                     /locus_tag="PKI07_00205"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOC49501.1"
                     /translation="MMKTSLFVLLGILALPALAQPARPVVIAHRGASGYLPEHTLESK
                     VLAHAMGADFLEQDVALTKDDVPIVIHDIYIETISDVAQRFPERKRANGHYYALDFTL
                     AEIKQLRAQERVNPRTGQPVYPNRFPPGKSSFQIATLEEELQLIQGLNKTSRRVVGIY
                     PEIKQPAWHRKEGHDISRIVLPILRRYGYATKQDPCWVQCFELDEVKRIRTELGWEGR
                     LLLLLAMNRKHPDGTSAEAWFTPAGMMEVAKVADGIGPELGAIVSGSSKATRKVTDFV
                     KHAHAAKLIVHPYTLRADDLPKFAESMDDALEVLFAEAGLDGFFTDFPDLPVKWLTAR
                     GLR"
     gene            complement(53181..54314)
                     /locus_tag="PKI07_00210"
     CDS             complement(53181..54314)
                     /locus_tag="PKI07_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOC49502.1"
                     /translation="MKAKPDINERYVIIMAGGRGERFWPVSRERMPKQLLTLLGGRSF
                     LQQAVDRVLALVPLKNILVITNAVQAPAVRQQLPKLPKGNVIAEPVGRDTTAAVTLGA
                     ALVGARSTTGVMAVLPADHVIPEEKKFQQVLGDAFDLAGRGQAIITIGIKPTEPATGY
                     GYIRVGEKLPPPVGVKSYKTAFYRAEQFVEKPPFDKALEYVNSGQYRWNAGMFIWSFV
                     TITEGLQKHQPEMHAACQRWFKVATQPARLNAVLAKEYPELKKVSIDYALMERAQNVI
                     VADGAFAWDDLGSWTALGRHLKADPEGNCAVADFIHVDGARNIIFDARSKARRTPIAV
                     VGLRDSILVQTDDATLLAHKSQAQKVKELVKRLAANPKLKKLM"
     gene            complement(54311..55111)
                     /gene="trpA"
                     /locus_tag="PKI07_00215"
     CDS             complement(54311..55111)
                     /gene="trpA"
                     /locus_tag="PKI07_00215"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOC49503.1"
                     /translation="MNRIVERFARLNRERQKGFIVYLGAGDPNLEATRQLALALDQLG
                     VDVLELGVPFSDPLADGVVNQLAAQRGLDSGTTPSRLLATVAAIRRDSQIPIVLYVYF
                     NLICRQGVESFIRDAAGAGVDGLLVLDLPPEEGADYEAKMRAAGLCNIYLVAPTTPEN
                     RIEMIVKRAAGFIYYVSREGVTGMQSQVADTITPMIAKIRAHTTLPIAVGFGISTPEQ
                     ARLVAQNAEAVVVGSAIVNQVALHGKAPDLIERVSGFTRPLLRAVKQL"
     gene            complement(55399..58326)
                     /gene="gcvP"
                     /locus_tag="PKI07_00220"
     CDS             complement(55399..58326)
                     /gene="gcvP"
                     /locus_tag="PKI07_00220"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOC49504.1"
                     /translation="MSVIEPASCDRNNSSPASVAAGPVNAHLPPPADRFRHRHIGPRA
                     SEAEEMLRLLGYTSLAALIDDAVPPAIRLRQPLQLPPARSEPDVLAALKKIAAQNQIF
                     RSYIGMGYYDCHVPAVIQRNLLENPGWYTPYTPYQAEIAQGRLEALLNFQTMVTDLTG
                     LEVANASLLDEATAAAEAMTLCHSLKPARSVFFVSAECHPQTIEVVKTRADALALRVE
                     TGDHRAFQFHDQVFGALVQYPNTWGEVVDYSTFIEQAHAAGALVAVAADLLSLTLLRP
                     PGEFGADIALGSTQRLGVPLGYGGPHAAYFATRDTFKRLLPGRIVGVSKDARGRPALR
                     LALQTREQHIRREKATSNICTAQALPACMAAMYVSYHGPAGLRQIAQRIHQRTVVLAR
                     SLERLGYEVGSGLFFDTLRIALGQKKAADLLKAAESQGMNFRLIDEHTLGISLDETTT
                     EQDLAGIIRVFHDGQTPAFELTNLVAEAEVAYPVPFARTSSYLTHPVFNRYHSETEMM
                     RYLKRLEARDLSLTTSMIPLGSCTMKLNAAVEMLPLTWSELTRLHPFAPLRQTRGWQV
                     LFQQLEEWLGAITGFAGISLQPNAGSQGEYTGLLVIRAWHASRGEGHRNICLIPTSAH
                     GTNPASAVMAGLKVVAVACDHEGNIDLADLRAKAEAHQANLAAVMVTYPSTHGVFEPA
                     IREVCEIIHAHGGQVYMDGANLNAQVGLCRPGDIGADVCHLNLHKTFAIPHGGGGPGM
                     GPIGVAEHLVEFLPGHGVVNLGGENPIGAVAAAPWGSASVLVIPWVYIAAMGGAGLTE
                     ATQYAILNANYIARRLQNYFPILYRGPASLVAHECILDLRQFKDVTVEDVAKRLMDYG
                     FHAPTISWPVPGTMMVEPTESESKEELDRFCDALIHIHAEITAIQSGQSDKHNNPLKN
                     APHTADQLAVESWDHSYSRQQAAFPAKWLYDHKFWPAVGRIDNVHGDRNLICTCSGLG
                     GLS"
     gene            58443..58871
                     /locus_tag="PKI07_00225"
     CDS             58443..58871
                     /locus_tag="PKI07_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOC49505.1"
                     /translation="MKSRTKTTQKQTRRITKQLAIFLDNRPGMLARVADALADAKINI
                     YAITTSDTVDHTVIRLVVDDYRRALHVCEERGSLVVEDDVLMVEGDNKPGEMARLARK
                     LAKAGINIEYCYSATLPGAGKGLLILRVSNPTKALKVLKL"
     gene            58924..60426
                     /locus_tag="PKI07_00230"
     CDS             58924..60426
                     /locus_tag="PKI07_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOC49506.1"
                     /translation="MSDPLLYEINTRCWLHRLSDRYSAPITLANIPADELARWRKLGF
                     THIWLMGVWAAGPLARAKALQYSDLRRAYDQSLPGWRETDVAGSPYAIGDYQVPPALG
                     GEAGLAAFRRQLHAQGMKLVLDFVPNHLGLDHAWVREQPELFVQSTGEAAGTFAQQSG
                     GQIRWLAYGKDPYFPPWTDTVQLDYRRDTTRGAMTELLASIARHCDGVRCDMAMLVLN
                     GVFAKTWKHFPVTTSPPVEEFWTTAIARIRQAHPDFLFLAEAYWGLEPELRRLGFDYT
                     YDKTLYDHLIAHDATDVQRHLLGLAAGQLAAGAHFLENHDEPRVAALLSLAEHRAAAL
                     CILGLPGMRFLHDGQLEGARIKIPVQLARVAPEAGQSEIQALYQQLLGTLPGTAVGRG
                     RAVLCQPRAAWSGNPTAANLIIVQWQQQTPEFDLVVVNLAPHRSQCYVPLSVPQLAAR
                     SWVMKDLLGSECYQRAGDDLQTQGLYLDLPPHGAQLFHFQPGELVKGARL"
     gene            complement(60498..61916)
                     /locus_tag="PKI07_00235"
     CDS             complement(60498..61916)
                     /locus_tag="PKI07_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOC49507.1"
                     /translation="MREFRGAWVATVANIDWPSRKTLSTQEQKTELLAILDRAVHLKL
                     NAIIFQVRPACDALYASRIEPWSEYLTGTMGKAPEPFYDPLAFAIEEAHKRGLELHAW
                     FNPYRARLRSAASPVAANHVSKTRPQLVREYGEFLWLDPGEKAVQDYSLSVVMDVVKR
                     YDVDGIHFDDYFYPYKARDRAGKELDFPDNASWQRSGVAGKMSRDDWRRENVNNFIQQ
                     VYKSIKTTKPWVKFGISPFGIWRPNSPAQIKGLDAYATLYADSRKWLINGWADYFAPQ
                     LYWAIEPPAQSFPVLLQWWAEQNPKGRTLCPGMNSTKVSGPSPSSSGWSPQEIVSQIR
                     LTRKQAGVGGHIHWNMKSLMRNNALGEALVRETYQQPALMPLSPWLGRATPAKPQLTL
                     ASPQRAGSLTLSWALAGPGKAWLWLVQTRSGGTWSTQILPGGQSSHTWSGTTPEVVAV
                     SAVNRNGEASAPSLFQSQSKTP"
     gene            62262..62966
                     /locus_tag="PKI07_00240"
     CDS             62262..62966
                     /locus_tag="PKI07_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49508.1"
                     /translation="MRLRSLLGCFVLLLALTVRAGIIYSPANNVNLTVNDGDPIGHTL
                     TATVSGYLPTIIDVRVTLNIEGVDGGGWNGDLYCYLTYNGTLVPLLNRVGVQTGDAFG
                     YGTIGMNVTLAGDPGGYDDIHWTEFPTSGGTYAPDGREISPGMPPSSAPDFDADGTIG
                     FSAYNGMDPNGTWTLFIADMAGGDQLKLVSWSLQITAVPEPVNVALGVFGSVFLVVAV
                     GRHQRVRKLVSQALGR"
     gene            complement(63128..64003)
                     /gene="argB"
                     /locus_tag="PKI07_00245"
     CDS             complement(63128..64003)
                     /gene="argB"
                     /locus_tag="PKI07_00245"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOC49509.1"
                     /translation="MQNLVAKAHTLLEALPYIQRFSGATFVIKYGGSFMDSPDAEVRH
                     RVARDIVFLEAVEINPVVVHGGGKAITRAMEKAGLQATFLQGQRVTSEATAQIADQVL
                     SREINPEVVAAINALGGLARSFAGPDIFHCRKAQVKGADGQPLDLGYVGEVIRVNTAP
                     LLDCLRQGITPVISPTARGEDGKVYNCNADVAAAQAAIALNARRLVFMSDVPGLLHNP
                     KSPDSVIPRLKISEVETLKQNGIVDKGMIPKVDSAVAAIRAGVEKVSFVDGRVPHSVL
                     LEIFTDEGIGTIVTA"
     gene            complement(64019..64939)
                     /locus_tag="PKI07_00250"
     CDS             complement(64019..64939)
                     /locus_tag="PKI07_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49510.1"
                     /translation="MMRHLARVLFLGVSAYAFAQAPPQPSARPRLAFLPDGTNGYTFN
                     TGVLQGQLRAGGKSIGLSEIVHLPTGTRLDHSMGLFGHYRVFSTNRRHGSAAWDWPSE
                     ATLCADGSVEVRWPSTAERPFELSAVYRWTTPDTLDLETRVLAKTRLPRFESFLASYF
                     TETFTNACVLTRIHGQKVFTPAGRGAGVWQAFPRDNKAASIIQDGRWKFPPSPVDWAI
                     RPRLARPLGLRSSPPTGLQAIIMAPPRDCFAVLTPFETEGHRSMYLSLFGQDLRPGQT
                     ARTRARLVIRTGLEKKDLEHLYAAYLRQIH"
     gene            complement(65046..66755)
                     /gene="ade"
                     /locus_tag="PKI07_00255"
     CDS             complement(65046..66755)
                     /gene="ade"
                     /locus_tag="PKI07_00255"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOC49511.1"
                     /translation="MRELQRKLSIARGERPAERLFKNARLINVLSGEIYRANVAVDDG
                     RVVGLGDYRARQIIDLKGAYLAPSFIDGHFHVESSMLTPREFARAVVPHGTGAVVVDP
                     HEYANVLGLDGIRYVLESSANLPVDFFVMLPSCVPATALETSGARVTPEDLRLMIGDE
                     RIAGVGELMNFPGVYLGLESELAKIQAGKGKNIDGHAPGLRGRLLNAYVLAGVQSDHE
                     STKLAEAREKLRLGMHVLLREGSTERNLATLAPLINSHNAMNCSMATDDKLAGDLVAE
                     GHIDHCVRLAIKKGVPPMAALQIATISTARHYRLRNFGAIAPRYWADFIVFDDLKKLV
                     VRQTYKKGILVAAEGEYLAPPRTLAPPPRSTMNLRCQAPDDFAVRAKGSSRIRVIEMV
                     PHQIVTRQAIETPRIEGGYVASDVMRDILKLVVVERHHATGNVGVGFVRGFKLRCGAL
                     ASTVAHDAHNVIAVGVTDADITLAIEELVKMQGGQVAVSDGKVRAALPLPIAGLVSHR
                     PLPEVIDRIADLNAAARAMGCRLATPFMTLSFLSLSPIPELKLTDQGLIDSVKMRSVS
                     LFV"
BASE COUNT        12228 a        20149 c        21667 g        13197 t
ORIGIN      
        1 gccatcgtcc cgccgccgcc gatcttgaaa tctcagcaag cggctgaaac ggcaagggtt
       61 aagaaatcga agcccgcccc cgccccggca tccaccgccg cccacaagac cgttccttct
      121 ccgtcctttc gagccatgta caaggggagt acatcggaag cccggcagga gtacatggaa
      181 aataagcatg agtccccttt gagcctcctt tgtacccccg ttgtacacgg cctaatggga
      241 gcgttaggct ggctggggcg ggtgggtcgg gtttgggcgc ggacctgagc cggaatggag
      301 ggatggacga cttgcggacg atgtaaaacg aggttccaag gcgggaggga gaggacgcga
      361 acgttcggca cagccggaac ggggggccgg gtgttgtcgc gcgccgtggt tttgggcagg
      421 atggggtcac ggttggcggg gatattccct cctctcgggc ggaggctgtc gaaaggcggt
      481 tgcttacttt gttctcattg caggcggcgt attcgctctg ttggggcgag gttggttgaa
      541 gatttagccc cgggctattc gcgcaggagg agtgattgac gagattggtt cgggaaccta
      601 tctcggcaat tatgctggct ttgggcttgt aataactgtt ttcaccgggc ttgttcacta
      661 tggccatcgc ctatcccgca cgctcacctc cctcctcgca accgcgctat cccaggcatc
      721 attgggtttc tgctgctttc aagttggcgc atgttcgtgc cttcggtatt tgaggtgatc
      781 ggaggggtta tttcttcagg ggctgaaaca cattcccgac gcagataccg gttgcccttg
      841 gcttgcattg gctgagggag cggctatgct gttggaagtc ttgaatcagc atggccagtt
      901 ttgttcttcc ttgaagctgc gcattagcgc cggattgtgg ctgctttgcg ccacgtcggt
      961 tttggcggca cttcccgcac tggtgccccg gccgcaggtt cttcagacca acgccggggt
     1021 ctttaccctt tgcccgcccc aggcaatccc cggacttccg accctggctc ccacaacgat
     1081 ccacgcggac gaagtcgggc gccccagcgg ggaatacctg gcgatgcagt tgttcaagtc
     1141 caccggatac cgcttccagg ttgagagcaa ttccggcgcg gcggcggtgt cccaggcgat
     1201 tttgctgacc accaatggcg ccctggggag ccttggcggt gaaggctatg aactgaccat
     1261 tgccaccaat gcgatcgtga tccgcgcgcc agcggcggcg gggctttttt atggcgcaca
     1321 aacgctgctg caagccctgc ccccggaaat ctacggcgcg cgtccggttt ccggcgtgcc
     1381 ctggacggtg ccttgcctgt atatcaagga ctggccgcgc tttccgtggc ggggattcat
     1441 gctggacagc gtgcggcatt ttttcaccaa ggacgaggtg aaagtggtgc tcgactcgat
     1501 ggcgctgcat aagttgaacg tgcttcactg gcatctcgac gatgattcgg gctggcggct
     1561 cgaaatccgg aaatggccgt tgctgaccca ggtgggcgcc tggcgcacgg atatgatgtg
     1621 gaacctgaac ccccgcgcca gccagtgctg ggacggcacc aactacggcg gcttttatac
     1681 ccaggcggag gcgcgggaaa tcgtggaata cgccgcccaa cggcatatta cggtggtgcc
     1741 ggagattgaa atgccgggcc attcttcagc ggccctggcg gcatatccgc agttcgcctg
     1801 cggctgtccg acctgctaca acggccccta cagcctgaat gtcaccagct atgtcggcgg
     1861 ggtattctgc atcgcgcgcc cggaaacgat ggatttcctg aaggatgtgc tcaccgaggt
     1921 catggaagtg tttcccagcc cgtacattca tatcggcggg gacgaggtga gattcaccaa
     1981 ctggcagaat cataacctgg accgggcctt gaccaacagc ctgggcatcg ctgatatgca
     2041 aaagtaccag ggccacttta cgcagcagat tgccaactgg atcaagagcc aggggcgcac
     2101 catgatcggg tggagcgaaa tcatgaatgg cggcttggtg accaatgcgg cggtaatgga
     2161 ttggcttaat acccgcgcgg tgcagacggc gaccaaccgg caatacgtcg tcatggctcc
     2221 cagcgcgact ctttatatca acaaatggga gacgggtgac cagaacggcg gcggagtggt
     2281 tttctccaat gaaccgcccg cccagtcggg gtttgtgcca ctggccagcg tttatgcgta
     2341 tgagcctatc cccacgaacc tcccgcccgc ttacacgaac tacatccttg gcgccgaagg
     2401 cccctgctgg tcggaatgga tcccctcgct gctgaacctg cagttccgga tgtttccgcg
     2461 cctctcggcg attgccgaag tgaattggac cgcccccgca ctgaagaatt ggatggactt
     2521 caccaatcgc ctggtcgttc acaagcaaag actcacccac atgggcatca actacaatcc
     2581 cagcgccacg cccccgcagt tgggcgcgtg gactccctcc ctgacgcccg ccaactacgc
     2641 cacgctgacc tgggatatca cggccagcgt gagcgcgccg ggggagataa acgtcagctt
     2701 ttgctggaag tcggggaagc atggcctgta catcgcatgg gccgcgctgc tggaaaacga
     2761 ggtggagata gaccgggaca cgcacgccgg atataccggc tacactccgg tcaagccaag
     2821 ctatgtgttg cggctgccgg cgtatcgtcc gggagcgacc tatcggctgg cggcttcggt
     2881 caaggggcgc gacgggaccg attcgcacgg cattgtgtac cgaaccaatt ggaactgagc
     2941 gatgaagcct tcctggatca aaatagttgc cgccgctgcg ctgctgctgg cgggcgcgcg
     3001 ggcgcaggcg ttgtccatcc gcacgctggc gggcggcgtg acgccgggcg ccctcaacgg
     3061 cttcggcagc catgcccgct ttaattatcc cggcggagtc gccgtggacg gcgccggcaa
     3121 tgtctatgtc gcggatacgt taaacagtat tatccgccgg attgcggcgg acggcggcgt
     3181 cagcacgctc gccggcctgg ccggcgaata tgggagttcg gacggggccg aggccgccgc
     3241 gcggttttat ggcccgcagg ctatcgctgc cgatggagcg gggcagcttt acctggccga
     3301 taccgtcaac agtaccatcc gcaaggtgac cccggcgggc gaggtcagca ccctggctgg
     3361 ggtgcccggc gatttcaaca gttttgatgg cgcgggcgct gcggcccagt tccaccatcc
     3421 cgaaggcgtg gccgtagatg ccggcggcaa tctttacgtg gcggacacgt ggaatcatac
     3481 cattcggaaa ataactcccg ccggcttggt cagcacgctg gccggcctgg ccgggtattg
     3541 cgggagcgcg gatggcacca acagtaaggc ccgattcaat cgcccggccg ggctggtggt
     3601 ggatggggca accaatcttt ttgtggccga caccttcaat catactctgc gccaaatctc
     3661 gcctgcggga aaggtgacca ccgtggccgg tttgcccggg gtttggggca gtgccgatgg
     3721 cgccaatagt gccgcccggt tttacctgcc ccagggaatt gcgattacca ccgacggcga
     3781 tcttctcgtg gcagattcgg gcaaccagac cttgcggaag gtttcgcccg tcgggacgaa
     3841 ctgggtggtc agcacggtgg cgggattgcc cggcctggcc ggaagcgtta acggctccga
     3901 cggtcatgcc cggttttgct ttcctgccgg tttggcgctc gacaacgcgg gctgcctgta
     3961 tgtagcggac gcagccaatc acctgattcg caccacccga gtggtcccgc ccacgctgca
     4021 gtttgctgcc ctggagggtc aaatggtgct ttgctggccc acctcagccg aggggttcgt
     4081 gttggagcaa tctccgacgg ttgacgcgct ggcagcctgg tcgcccgcga ccaacggcat
     4141 cgcgattctc ggagacagtt ttatttgcac caacagccct ggcgcggccg ctttttaccg
     4201 gctgcgcttt ccgtaaaagc cgccgtggtt gatggggcta gtggtatatt gccagccgga
     4261 cagattgggg ggcaaagggt tcgatatgga aggtgttgac ccccggatgc aggtgcgagc
     4321 cgatttccag gcgcgacggg cgcccaatga acccgcccgc gtctttgccg ttcacggtca
     4381 ctctggcggc ggtctctggc gagatgcggt caagctccag gtaaatccgg gtgctggtta
     4441 ggtcggacgc ggagagttcg cagcggccaa ggaagggatc agcctgaacc gggctgacgg
     4501 tgatccggcc gccgagcatt ccccagatgc cgtcgccgaa aggaacgagt tcccgcgccg
     4561 ccggccaggc ccggtcgtcg aagccggatt cggtccactg cgcggctttt tcccgggaaa
     4621 ccttccagga taggtctatg cgctcagtca gcaccgaacc ggaggtcaac tcgacggtga
     4681 gtcgtcccag caagccggcg gggttgggtt tgtcgccggc gttgactgcg acgatggcaa
     4741 gctgattcgg gccggaggac aggaaacggc ttacgtccag ttccactggg ttgcgccagc
     4801 cctcggcgct gttgtcgccc tggccggctt ccttgccgtt gataaagagc gtgaaacggt
     4861 tgtccgcagt gccgacgaaa acggctttgc ggatgctggc cccggcgggg agattgaact
     4921 gcttgcggaa atagcgcggc ccgggcgggg catggtgagc cgcgtcggtt tccggatacc
     4981 agacccaggg gcagccgcgc agcgccagtg ccgcgggatt gtcaagggat ggcaaggggg
     5041 ttggcgcggc gggagccgga tcgcgcgcca cggcgatggt tcggggaggc gcagcgctgc
     5101 cggattccgg gcgcggcggc aaggcgcgcg gctttggctg aaagacgagc aggacgcttt
     5161 cattgggttc cagcgtcagg cttagttcaa cctgccggcc cgtgtgcgag tagggcaggg
     5221 cggtgatctc gttgcgcatg gcgtcccagc attcaggcgt gccatcggcg gtgatgcggc
     5281 agcggaaggt gcggggcgcg ccttcatgat tctggttggc gatgaagaac acgtcgcggc
     5341 cggccttgac ccggtgcagc acgtgaagcc agtgatcggt cttgccttcg agcacctcca
     5401 gggtggggcg gatgccggca tccccggcca gcacttgctg gagttgttcg ggcgtgggct
     5461 gcgccggcag caagtaggag cggccgccgg cagggctggt tttgcagacc gccaggccgg
     5521 gcgtgggctg tccccagagg gcctcgcgca gccgcgttat gtccgctgcc gttttgccgg
     5581 gcgtggcgga tttagccggc agaaagccgt aacccagcac gacgccgcca cggtcgaaga
     5641 attgtttggc cttggccagc gtggcgcagg ggataacttc caccggcggc acgatgagca
     5701 cgcggtagcg ttcctggcgc agttggagtg tggcccggcg gattcgggta tcgttttcaa
     5761 ataccgcata cggcagccag tcgcagtcgt agcgggcgtc ctggaggctt tcgctgatct
     5821 ggtcgggcaa aatgtgctgg ccgcagtgcg cgctttggcc gaggtaaagc agcgccaccg
     5881 ggcagacgtg gcggccgccg gtgagcatca ggctcaatcg gctggtgtag tcggcgaaca
     5941 cgcggtaaag cggccaacgc ggttcaaagc cgccgttgta aaaataggga ggacaatcgg
     6001 tgtcccgcgg ggcgcgcggg ttgaacgagt gcgggatgag gaagttgacg cccgagacct
     6061 gcatgtggtc ggtccaccat ttcatctccg ggtaggtgag ggtctggccg cgcgcgccga
     6121 agatttccac catggccacg tcgtccggtt tgccgtaggc atggctgatc gagctggcca
     6181 gcttgggggt ttgccagcag ggcgcgtcgc gcgcctcgcg ccgccccatg acgaactggt
     6241 cgaacacggc atctatgccg cccatgtcgc cgtggccttg caggcgcatc atgtctccgg
     6301 cgcagtaatc gggatgctgg taaagccggg aatgttccat aaagtggccg gtggacttta
     6361 cgccatgctg ccggcaccac cgggcgatgc cgccatacat ggtgcggccc caggcctccg
     6421 ccagggcgtc caggtattgg tagcgcgccg cgatttgcgc ctcgcccgcc agttccgatt
     6481 tgtaggcggc gtaggccagc ttccaatcca cgccccactc agccagcacg cggttgagtt
     6541 cggtgcccca gtcgccggga gtttcgggtt catcataaaa gaagccccga atggtcttcc
     6601 caaaatcggc tccgtagcgc tcgaagtgag gctggtaaac ggtttgcaga taccaatcca
     6661 cgcaatcccg gctcgcgccg tccaccgtga gttgccggcc gccgacctgc aggaggccgg
     6721 gggcctgggt atgggtgaat ttgaggatgc gccatttgcc gccgggaacc ggccagcgaa
     6781 gcgtgcccgc atgaatgtga ggagcgagat ccaccagcga gtcggcgtct atcttgcctt
     6841 cgacgtccag ccgcccggcc agagtggcaa tataccgttc gccgccgtgt ccctccgcct
     6901 cgaaaagccg gggcccttcc acctcgaccg ccgccgctgc cagccgtttg gccgcgtagc
     6961 gcggcggcac tttgccggcc accgcctggc tcggccacca tttttcgtca aagatccaga
     7021 gctggagatt gtgctgtttg gcggcgcgca ggcagatgtc cagatcccgc caccaaccgg
     7081 cgccgagcca gtcgttgtgc ggtcgcgatt cggtggtaaa gcagccattg ccgccctcgg
     7141 ccaccttgcc gacgtacagt tccagccgct ccttgctttc atcaccgtgc agccagaaca
     7201 gcggtccggt aagccgccgg gcttcagcgg gcaactcccg gaattgcttt tccaaggccg
     7261 ccaggctcga accggttgcg gcgaccgccg ccagagacca agccagcgca cagaacgccg
     7321 cggcggcaat gcaaacggag gagaaaagag gcttcggcgt tttcatgatt cgttgcaacg
     7381 ccctacatga ctccagacaa tggcccgcgg atcaatgcca aactttacgc tccaaagagc
     7441 ggtgaggaaa accaacccgt atatgcgcgc agcgcgcacc gctacagcaa aaccggcagg
     7501 cgtttgatgg cctgccggtt gaagcttccc ggggcggtcg cgccgcgccg gttattgcga
     7561 ttcctcttgc ttcgcttcgt cggcggcgtc gaaggcgtgc tggagggcaa cgagatcttc
     7621 gccgggcttc aaggcgtcca ggatggcctg ctcggccttg agctgttcag tggagtagtt
     7681 ggcggccttg atggacaagc cgatgcgccg gtcgctgcga tcaatcttga tgacgcgggc
     7741 ggtgatttcc tggccgacct tgaggacgtt tttgatttta tcaatgcgct cctcgctgat
     7801 ctgggaaata tgcaccaggc cgtcaatatc atgctgcagg ccgacgaaag cgccaaagct
     7861 ggccagcttg gtgacgtcgc cggtgacgag gtcgccgacc ttgtaaatct ggtcaatgcc
     7921 ttcccaggga tccgcagcca gttgcttcat gcccaccgcg atgcgctggt tggccttgtc
     7981 cacttcgagc accaccgctt cgacttcgtc gcccttcttg accatttccg aaggatgatt
     8041 gattttgcgg gtccaggaca tgtcggagac gtgaatcatg ccgtctatcc cttcctccag
     8101 ctcgatgaag gcgccatagc tggtcaggtt gcgaatcttg cctttgaccc gggtgccggc
     8161 tggatacttc tcctgcgcct ggtcccacgg gttggcttcg agctggcgca ggccgaggga
     8221 aatcttctgc tcctcgcggt tgatgccgag caccaccgcc tcgatctcct gatccgtttt
     8281 gagcatgtcc gaggggcggg ccacccgctt ggtccaggag aactcggtga tatgaacgag
     8341 gccttcgacg ccaggttcga cttccacaaa ggcgccgtag ggaaccaggc tgaccacccg
     8401 gcctttgacc ttggcgccca ccggatactt ctcttcgatg gtatcccacg ggttggtgag
     8461 tttctgtttg aggccgaggc tgatgcgctc tctttcccgg ttaatgtcga gcaccacgac
     8521 gtcaatatcc tggccgacct tgagcatctc cgagggatgg ccaatgcgtc cccagctcat
     8581 gtcggtaata tgcagcaggc cgtcaatgcc attaagatca atgaaggcgc caaagtcggt
     8641 gaggttcttg actgtgccct tgcgaatgtc gcccggggtc atttcggtca gcagcttctg
     8701 gcggcgctcg gcgcgctcct gctcgatgag ttcgcggcgg gacaggacga tgttctggcg
     8761 ctcctggttg atcttgacga ctttgaactg gtacgtgttg ccgacgtatt gggcgaggtt
     8821 gcgagaaacc acgacgtcaa tatgggagga cggcagaaaa gcctcgacgc cgatattgac
     8881 gagcaggccg cccttgacga cagccttgac cttgccggcg atggttccgc cctcgttgca
     8941 aatggtgagg atgcgctccc aattctgctt gaactcggcc ttctccttgg agagaacgac
     9001 catgccttcc ttgtcttcga gcttctcgat caggacatca atctggtctc cgactttgac
     9061 ggccttaagg tcttcgaact cgctggccgg gatgacgcct tcactcttgt agccgatgtc
     9121 caccaacgct tctttggcgc cgatttcgat gatggttcct ttaacaatgt tccccgccgc
     9181 aaagggcggt ttgcccgctt gccgcagggc ttcttccata gtcagcataa tttagcaact
     9241 aactttcttc gatgaatcta agggaggcag ggtcagcgcg aggccttcca agactccatt
     9301 gcctaacgga cccgtcgttg ctgtggcaac ggaggttgag ggttaggttt ttcgttaact
     9361 tctctttctc agccgcaaag gacatccgca cacacggtgt ccaggcaatg accagactag
     9421 cagatcgctg ttcggatgca agcggaaata tcaggcattt tcgccttgcc aaacgcgcct
     9481 tcggagcgct aaataccgcc ccaattcaga taaacgctat gcatgtgcaa ctcgcctggc
     9541 cggattactt tattctgctc gcttatacag gatttgtcat cggcatcggt tttgccctcc
     9601 ggaaatacct gaaaagctcg tcggacttcc tcacctcggg ccgttcgatt ccgacgtggg
     9661 tgaccgggct ggcgtttatt tcggccaatc tcggggcgct ggaactggtg ggcatggcag
     9721 ccagcggggc caagtacggc atcgccacca gccatttcta ctgggtggga gccatcccgg
     9781 caatggtgtt tctggcgatc ttcatgatgc ccttctatta cggctcgaag gcgcgctccg
     9841 tgccggaata tctgaaaatg cgcttcgatg agcgggtgcg ggcgctcaac tcgattgcct
     9901 tcgcggtaat gaccgtgttt gcgtcgggca tctcgatgaa tgcgctggcc aagctgctca
     9961 accaactgct cggctggaat taccacgtgg cgctgtggat ttgctcgggc gtggtgctgg
    10021 tgtatgtcct taaaggcggt ctgacctcgg cgatctacac cgaagtgctt cagttcttca
    10081 tgatcgtgct cgggtttgcg cccgtggtgt acctggggct taaggacgtg ggcggctggg
    10141 gcgccatgaa ccaggccctg caaggggtgg cggtcgatcc cgcggcgttg cacctcaaag
    10201 aggcgggcac cacctacgcc ggcaatgcct ggtccagcgc ctggcagccg ctgctggccg
    10261 gccccgcgca caacccgatg ggcgtggatt ggttcgcgat ggtgtttggc ctcggctttg
    10321 tcctgtcgtt cggctattgg tgcaccaact tccttgtcgt gcagcgcgcg atggcggccc
    10381 gcaacatgac cgccgcccgc aacaccccgt tggttgccgc cgtacccaaa atgctttttc
    10441 ccgcgctggt aatcgtgccc ggaatgattg ccgtggcgct gacttcgatg cccggcaaaa
    10501 actatcgcat ccccccgccg atcattggcg acgaggttta tacccgggcg gcggaggtcg
    10561 tcaaaaccgt cggcgccctg gaagctcccg ctgccttcga ggccgtgcgc gaggccatgg
    10621 gcaagaagat ggacccggtt aaagtcgccg cgctgatccg ggacgccggc cacctgagtg
    10681 aggggcagat taaggacggc ctctatgagg cggtcgccga gtacgactac gacggcgtga
    10741 ttttgtccct ggtaaagaag tactgtccga ccggcctgct ggggctggcg ctgaccgcgc
    10801 tgctggcctc gttcatgtcg ggcatggccg gcaatgtcac cgcctttaac accgtctgga
    10861 cctacgatct ttaccaggcc tacatcgccc cgaaaaagag cgaccagcat tatttctgga
    10921 tggggcaggc ggcgaccgtg gttggcattt tggtcagcat cggggcggcg tattttgcct
    10981 cgatgtacaa taatgccatg gatatcattc agctcgtgtt cggctttgtg aacgccccgc
    11041 tgtttgccac cttcctgttg ggcatgtttt gggcgcgctg tacgggcacc ggcgccttcc
    11101 tgggcctgtt tggcggcatc ggcggttccg ccctctttca tgcctgcacc acggcggtca
    11161 gcaacctgga tgcatcgggc cgcgtggtca acgggatgaa aggcggctac ctgggcgtgg
    11221 tccagagctt cccgagcgaa atggctcaaa acttctggct ggctggtttt gcctttgtcg
    11281 tgtgtttcac gctcacgctc gttatctcgc tggtgacccg ccgcaccaag accaacgaag
    11341 agttgaaggg actggtctat tccctcacgc ccaaaatcaa ggacgaaggc gcgccctggt
    11401 atttgcgccc ggcggtagta ggcaccgccc tgatcgtcgg ttgcgtcatt ctcaacatca
    11461 tcttctggta aacgaaagga acacgttatg ggactggata ttcgctggcc gattggcctc
    11521 atgttctcgc tggtcgggct gctcctgacc cttttcggac tctgcacccg gggcgatgcc
    11581 gagatgtatc agcggtcgct cgatatcaac atcaacctga tctggggcat catcctgctc
    11641 ttgttcggcg cttttatgat catcatggcc ctgcgcaaaa agcccgatcc cgacgccctg
    11701 aagtcgaagt agccattcat gctcgccgcc ccgtacgcat tccggatgcc ccggacggag
    11761 cgtcggcctt cggggctgcc cttcgaatcc cgttttccgc aaacgggcag gaatggccgc
    11821 gcgctccccg gcccgtgcgc gagccgcggt cgcccggagg aatgtgaatg aaagcgacgg
    11881 ttgtccgaac cgccttcggc aagctccctt ccgggcgcgc cgtggacctg tttacgctga
    11941 ccaatacacg cggcctcgtc gccaaaatca cgaactacgg gaccattctc accgaattgc
    12001 acgtgcccga ccgccacggt cgccgggccg atgtcgtatg cgggtttgac catctcgaat
    12061 cgtatctcaa gggccatccc tacttcggct gcaccgtcgg ccgcgtagcc aaccgcattg
    12121 ccgggggcaa gtttatgctc gatggccggg cctattccct ggcggtcaac aatggcccga
    12181 acgcgctgca tggaggcctc cgggggttcg acaaggtgct gtggaaagcc acgccgctgc
    12241 gcgaggccac ggtgcgtttc gattacacca gcgcggatgg cgaggagggt tatcccggcc
    12301 ggctcgcggt tcgcgtcata atgaagctga ccgaggacaa cgagtttctc attgattaca
    12361 cggccaccac cacccggcct accccgctca atctcaccaa ccattcttat ttcaacctgg
    12421 ccggccgggg cgacatcaag gcccaccagt tgatgatcgc cgcggatttc tatacgccca
    12481 aaagcccggc cgacgtgccg accggcgaga tccgcccggt caaaggaacg ccgcttgatt
    12541 tcacccggct cacggccatc ggcgcgcggt ttgcgcgcct tggcggtcgg ccgcaaggct
    12601 acgaccacaa cttcgtactg cggggcgacc ccgccaactc aggccagccc gcctttggcg
    12661 cccgcgtggt tgagccccgt tccggccgcg taatggaggt gttcaccacc gaaccgggct
    12721 tgcagtttta cacggcgaac tggctggacg gttcgctcat tggaaagggc ggcttttctt
    12781 actcgcggca ctgcgggctt tgcctcgaag cccagcattt ccccaatgcg atcaacatcc
    12841 cccatttccc cagcaccgtt ttgcgccccg gccagaccta tcaccagctc accctccacc
    12901 gtttttcgac tgcttaacgc gctcgcgcgt catcgttcat ccctatgacc cttaacgaac
    12961 ttgcccggca aatcgccctg gacttcaaac aaacctgcaa ccgcgagcca caatggatcg
    13021 tcgccgcccc cgggcgcgtg aatgtcatcg gcgaacacac cgactacaac gacggctttg
    13081 tccttccgat ggccattgac cgctacacgc tcatcgcggc ggcgccgaac ggcacaaccc
    13141 aaatcacgct ccgcagctcc gccggccagg gccaggcgcg tgtggatctg acctcgccgc
    13201 tccagccggg cacgaaaggc gattgggcca actatcccct cggcgtcatc gccggttttc
    13261 tcgcgcgggg ccttcgtccg cccggttttg acgctttgat ccactccacc gtgccgttgg
    13321 gcggcgggct ttccagcagc gcggccctgg aagtggccac cgccacgctg ctcgaagtga
    13381 tcaccggcca gaagctggac ccggtggaaa aagccctgct ttgccagaag gcggaacatg
    13441 attacgccgg ggtgccctgc ggcatcatgg accagttcat ttcggtcatg ggcaaggaaa
    13501 atcacttgct gctgctggat tgccgttccc gccggccgga gctggtgccc atgagcgatt
    13561 cctccgttgc cctcctcatc gtcaacacga acgtcaagca tgaactgacg ggcggcggct
    13621 acgctcaacg ccgcgcccag tgcgagcaag ccgcccaaat tctgggcgtg ccttccctgc
    13681 gtgacgccac cgaagacctg ctccaggcgg cgcgcagccg gatggatgac gtcgttttcc
    13741 gccgcgcccg ccatgtcatc ggcgaaatcc agcggaccgt ccaggccgcc gcagaggtgc
    13801 gcgcgtcgaa ctggccggtg gtggggcaac tgatgtacgc cagccatgcg tccctgcgcg
    13861 atgactacga agtgagttgc gccgaattgg atgtggtggt ggacattggg cgggcgattg
    13921 gccccgcagg cggggtcatg ggctgccgga tgaccggcgg cggctttggc ggctgcgcgg
    13981 tggcgcttgt ccgcgccgat caaatggcgg cgatctccca gcggctcgcc gccgagtacg
    14041 agcaacgcac aaacatcaag cccaccctct ttgtctcacg ccccgccgct ggggcaatgg
    14101 ttttgaagcc ctgatccggg cgcaacgccg gtccagccca gcccgcgcgc aacttccgcc
    14161 gcgtcagtca ggccggcttg cacgagggcc tgggcgctct ccgccagcgc cggatgcgca
    14221 accaggccgt ccagatcccg ggtagcaatg cggcggcgca gggccggggt gaggcgcaac
    14281 cactcgacca gcggcagccg cccccggtag ccggtgtcca gacaggcggg acagcctttg
    14341 cccgcgcacc gttcgcagcg gcggcgcagc aggcgctggt tgagcacgag ctcaagcgac
    14401 gaggccacgg ctgagtggtc cgggcagagc accagcaaac gctcgaatac ccccgggcag
    14461 gagccggcat gcagcgttga aatgacgagg tggccggtca atgcggcgcg tacgaccagg
    14521 ttggccgttt cttcatcccg aatctcgcca atcaccagca cctgcgggtc ctggcgcagc
    14581 agatgccgcg ccgcccgggc aaagtccaat ccccgcgctt cgttcacctc agtttgcata
    14641 atgcccggga tgacctgctc ggcagggtct tccacggtga tcacgtgccg cccgccttgc
    14701 tcggcgagat gccggaggca ggcgtaaatc gtcgtcgttt tgccgctgcc ggcggggccg
    14761 gtcagcaaca gcaagccggc ggtttgacgc agaaaccctt ctaattccgc gagcgccggg
    14821 gccggaaagc ccagttcctc gagccggcgc gcccccgggg cgtcaaacaa gcgcagcacg
    14881 atcttttccc cgctgaccgt gggataagtc gcgacccgga tatcggcgcg ggcgttgagg
    14941 tcagcccggt caatccggcc atcctggggg agggattcct ggtaagtctt gagccgggcc
    15001 aggaacttga cgcggccaaa gacacgctca gcaatggccg cgggcagccg gtcggtgggc
    15061 gtcattacac cgtccaggcg gaaggtgacc gcggcgccgt ccgatagcat ttgcagatgg
    15121 atgtcgctgg cgccggcctg ttcggcttgc gcgaccaaat gttccaaaat ccggggggcc
    15181 gattcagcgg tgctcgcggg attttcagcc ggcgtcatgc gatctttggc gttcattccg
    15241 gtggttatgt ttccggggcc gggaccgggg cgttcgttcc tgcggcggaa gccggcggcg
    15301 cggcgatatc ggcaccggcc atttcctcgg agagaatggt gatggcgatg cggcgattga
    15361 gggcgcggcc ctcgggcgtc gagttgtcgg cgacggggcg aaactcgccg tagcccaccg
    15421 caccaaggcg gcggggatcc acgcctgccg tctctgtcag gaagcgcacc gccgccgtgg
    15481 cgcgggcggt cgagagctcc cagttgctcg ggaaccggct gcgagccgag gcgcgaatcg
    15541 gaacgttatc agtatggccg atgacgtgga ttttcaggtt cggatgagcg gcaaggatgc
    15601 cggcaacctt gcgcagcacc gccgacccgg cggacttcaa atcggcctcg ccggaatcga
    15661 acataacccg atccaggatg ttgacggtca gcttgccttg caactgggag atggtcacat
    15721 ccttggattc aagcgccgcc cgcatttcat cttcgagggt ctgatgcgta tgaaggaccg
    15781 tttgcttttc ggcctgcaac tcgccgatgc gcgcctggct ttgggccagt tggtccttca
    15841 gcccttcagc gaccgcccgc agccgggccg cgtcggcacg ggcggcggcc atttcggcgc
    15901 ggaccttttc cacctcgcgc ttcttttccc aggaagccaa ccccaacaac cccagcagca
    15961 gggccacggt cagtccggcg gcaatcatcc aggtttcttt gggcatagac gatcagcttt
    16021 ttgaagatag tagcccgatt ccgttaaacc gaaaagcgct ttcccgcatg cgaaagctcc
    16081 tccggcagcc tcccggacag ccgggagttt gagcggtttc gggtttggaa ataagaccga
    16141 ttaaaccgcc ttgggtggaa aaagcggact tgaaacgttc accggggcgc acgggactgg
    16201 gccggaggtg cggtttggag gaacaggtcg caaattcggt tcattggcgg cgttcacgga
    16261 cggcgtgagg tgaaattcca gcgttcgact ggttgaaccc ggcacagttt tttccaaagg
    16321 aggtttttct ttgaacgatc gggcggaaaa gttatctaac gtcccgagaa ggcctggctg
    16381 ctcgcaaacg gacgggccag cctggcggag aaaccacttt acattgaagg tggaattggt
    16441 tatctgcaca ggtcgttttg ccaaaagttt aggaaaagca ctatgattaa ggttcaaaat
    16501 ctagcgaagc tatttgggac caaacgggcg gtggatgacg tctcgttctc ggtgaaccgg
    16561 ggcgaggtgc tggggtttct cggacccaat ggagccggca aatccacgac catgcgcgtc
    16621 attaccggtt ttttgccgcc gtccgccggc tcggtgacgg tggggggctt cgatgtcgtc
    16681 gaacacgcaa ttcaggcccg gcggttgatc ggttacctgc cggaaaacgc gccagcttac
    16741 acggacatga cggtgctggg atttcttaat ttcgcggcgg agattcgcgg cttgcgcggg
    16801 gaggcgcgga agcaggcggt cggccgcgcc gtcgagatgt gcttcctgga atcggtgctg
    16861 caccagagcg ttgagacgct ttccaagggc taccgtcatc gcacctgctt tgcgcaatcc
    16921 ataattcacg acccggatgt gctggtgctg gacgagccga ctgacggcct ggatcccaac
    16981 cagaagcatg aggtgcggca gttgattcgg cggatggggg agaagaaggc catcattttc
    17041 tcgacgcata ttctggagga ggtggacgcc gcctgcacgc gggcgatcat tattgaccgg
    17101 ggcaagatcg ttgccaatgg cacgccgcaa gagttgcggc agaagtccga atgggccggg
    17161 gcggtgaccc tgcgggtccg gaacgtcgcc gccggcacgg tcagcacgcg gctttatcag
    17221 gtgccgcaag tcaagcgggt gacggtggtg gaagacaagg atggccatgt gacggtccgg
    17281 gctttccccc ggagcggcgc caacggcgcg ctggtggcgg ccgtcggcga ggcggcgcgc
    17341 gaatggcaaa tcgagcaatt gcaggccgaa gaaggccggc tggatgaggt ttttcggagc
    17401 attaccatgc ccgacacggc aaaggaggca gcataatgaa cgcctggcat aatgtttgga
    17461 ccattacgaa gcgagaattg ggcgcttatt tcacggcgcc gctggcttat gtgtttatcg
    17521 tgatcttcct gctgctgtgc ggttttttca ccttctttgt gggcggcttc ttcgagcggc
    17581 aggaggcgtc cctggtacgg cccttctttg actggcatcc gtggttctac ttgtttttgg
    17641 tgccggcggt ggggatgcgg ttgtgggctg aagagcggcg cgtgggcaca atcgaactgc
    17701 tgctgaccat gcccatcacc gcgtggcagg ccattgtggg caagttcctg gcctcgtggc
    17761 tgttcctggc gctggccctg gcgctgactt tccccgtggt gctcacggtt aactacctgg
    17821 gcgatcccga caacggggtg atcctgacgg cctacatcgg aagctggctc atggccggtg
    17881 cctacctggc gattagttgc atcacctcgg cactgacgcg cagccaggtg gtcagtttca
    17941 tcatttcgct ggtggtctgc ctgttcctga tcctggctgg ttttccgccg gtgatcaacg
    18001 tgttggagca ggtggcgccc cgcctggtgg atttggtgac gtcgttcagc gtgatgaccc
    18061 actttgaagg attccaaaag ggagtgctcg attcccgcga cctgcttttc tttctgtcgg
    18121 tcatcgggtt ctcgctcttt acgaccagtg tgatcctgcg cacacatcgc gccggttgaa
    18181 tcggaggctt agatgcaaaa gaaatcattt cagacaattc tctactcgac ggtcggcgtc
    18241 ttggtgatgc tggtcatatt ggtggcgttc aacttcatcg ccggcgcgct gcgcatgcgg
    18301 ttggatctga cgcaggagaa ggcctacacc ctttcggacg gaacccgggc catcctcaag
    18361 aagctggata cgccggtgac gatccgcttt tactgcaccc agagcgaaag cgcgacgccg
    18421 gagacggttt ttctcaagag ctttgcgcgc aaggtcgaag acctgctgac cgaataccgc
    18481 caggtggccg gtggcaagat caagatcgag aaatatgacc cgcaaccgga ttcggatgcg
    18541 gaagattccg cgcggctgga tgggatcgag ccgcaggcgc tggccggcgt tgaccggttt
    18601 tacctgggcc tggcggtaaa gtgcctggac gaggtgcaat ccattccgtt cctggcgccc
    18661 aaccgcgagc ggttgttgga atacgatttg gcccgcatca tcgtgcgcgc caccaacccg
    18721 gagaagcccg tgatcggcgt catgagcccg ctgccggtgt tcggcatgcc gtccaacccg
    18781 atgatgcagc agatgggcca gcagggcagc cagccctgga ccatcatcag cgagttgaag
    18841 aatgacttct ccgtccggcg catcggcatg gatgcggaca agattgacga cgacgtcaaa
    18901 ctgctggtgg ttattgctcc gaaagacatc accgacaagg cccagtacgc cattgaccag
    18961 ttcatcatgc ggggcggcaa actgctggct tttctcgatg cgcagtgcct ggcggatagc
    19021 cggcagcaga atcagatgat gatgaatatg ggcggcggcg gctcgtcgct ggacaaactg
    19081 ctcaaggcct ggggcctcca gttcgattcg tccaaagcgg tggcggattt gaaatacaag
    19141 atgcaactgc gcgggcgcaa cggcgaacca caggaggcgc cggcctggct cggtctgacg
    19201 gcggatgcca tagaccggga tgacgtggcc accagccaga ttgacaacgt ctggctgccg
    19261 ttgtgcggcg cgttcacggg cacgccgcag gacggcctga agcaaaccgt cctgctgcac
    19321 agttcgaaag actcgcaact ggtggacgcg atgctggcca acctctccgg cgagagcacg
    19381 atgaaggagt tcaagccctc cggcgtgaat tacaacctgg cgattcgcct gacgggcaag
    19441 ttcaagaccg ccttccccga cggcgcgccg caggacgcaa agagcgaaga tgaaaagaaa
    19501 gacgaagcca aaccggacga gtcaaaggcg ggcgattccc tcaaggaaac ccggagcgat
    19561 aacaccgtgg tgctgtttgg cgatgccgac ctgcttttcg accccttcac gattcggcgg
    19621 attgacagcc ccttcggcgc cttgcagatg ccgatgaacg ccaacctcaa cctggcccag
    19681 aacattatcg agcagatgac cggcgacaac aatctcattg ccgtgcgcag ccgggccacc
    19741 ttaagccgcc cgttcacccg ggtgaaagag atcgaggccg ccgccaacaa gaaattccag
    19801 agcgagatca agcgcctgga ggacagcgcc gccgaagcgc agcgcaaagt caatgaattg
    19861 caggcgcaga aacaggacaa agaccagcgc ttcatcctgt ctccggagca gcgccacgaa
    19921 ctggagaaat tgcgcaagga ggaagtggat acgcgcaaac gcctcaagca agtgcagaag
    19981 gatttgcgca aggaagtagt atcgctgcag acgcgtctca aatggatcaa tattctggca
    20041 gtaccgctgg cggtgaccgc caccggggtg gttattgcca tggtaaatcg caggaagact
    20101 tcggcaaaat gaatcggaaa cagcttctta tcctattggt gttggtggtg gtggtgggcg
    20161 cggccggcct ggtcgtgtac cagcggaacc agagttcatg gcagggcggc gggcgcgagg
    20221 gcgactcccg caagctcctg ggggaactgc cggtcaatga tgtcgcggct atcctgatcc
    20281 agcgcggcac gaaccagttg gacctggtcc gcattgacaa ccgctggcgg gtgaagcagc
    20341 gcgacgatta cccggccaac ttttccgaga tcagcagttt cctgctcaag gccgccgacc
    20401 tgaaggcggc gcagacggag gaaatcggcc cttcgcaatt agggcgctac caattgcttc
    20461 ccccggggcc ggaaaccaac acggccgtgc gggtggaact gcgcggtcag gacggcaagc
    20521 cgattaaatc gctcttgctg ggcaaatcgc acatgcgcaa agcggcgggc cgcgcctcgc
    20581 cgatgggcga gatggaagag gatgaaggct ggccggatgg ccgctatgtc atggtcggcg
    20641 gccaggtcaa aacggtcgcg ctggtctcgg acccgctcag caatctcgaa cccaagccgg
    20701 agcaatggct gaacaaggac tttttcaagg tcgagaaaat ccgttcgatt gcggtcacct
    20761 acccggccgc caccaactcc tggaaggcca gccgggaaac cgagagcgcc agcgaatgga
    20821 agctggcgga agccaaaccg gcggaacaac tggatcccgc caagacctcc ggcttcgcct
    20881 atgcgctcag ttcgccctcg ttcaacgacg tgctgccggc ggataccaaa ccggaacagg
    20941 ttggcctcaa ccacccgacc acgctaaccc tcgagacctt cgacgacttt acttacagcc
    21001 tcaagatcgg gcagcggacc aatgacaatc ttcccgtcgt cctggcggtc acggcgcaga
    21061 ttccgaagga acggacgccc ggcaaggagg agaaggacga agacaaggcc aggctggaca
    21121 aggaattcaa agagaagcag aaaaagctcg aagacaagct cacccaggag cagggctacg
    21181 gcaaacggat ttacctggtt tcctcctgga ccctggacgc gttgctgaag gaacgccacc
    21241 agctcctgct cgagaagaaa gaagagcccg cgggagaaga gaagcccgca gcaccgccca
    21301 ccaagcaggc cgaggacctg atggagcctg cggtggagcc caagtagggc gcccggaaga
    21361 ttcccttggg cgtccctctg gccgggggcg cgggatgggc ggtttgctct cacccgccgc
    21421 gcccggaagc gcctcgcaaa ggccggcagc ccaggcaggc ctgtgactgc gcttcgccga
    21481 cgaaaacggc tgctttaccc ctcaacattc tgcccgcccg ggcggcaacg cgcccctttc
    21541 cctcgcgcat catggcacgg agcgtgcgga ttgtccgggc ggctgcggcg ccgggtttgg
    21601 cggcgcggcg aacgaacatg acaatgaaag cttttagcgc gtctggttgg gcaacgaaag
    21661 cgctggcggg gttgatgctg gctgcttatg cggtgctgcc ctcgtcagcc gttggagcca
    21721 cggaatccaa actggaagtg ctccaaacca agacagcgac gtacaccaat gtgacagtga
    21781 ccacgaaggc caagggctac attttcattg tccatgaccg cgggatggcg agccttaaag
    21841 tcgcggacct gtcccccgag gtccagcagc agcttggtta ttcagtggcg ggcgcggcca
    21901 aacattcgac caacacggcg gtggcctgga ccaaacgcga agtggcccgg ctggccgggc
    21961 cggaggtgaa ggccttggaa acccggcttc agcaaaaatg gtcgggacac ccacccgccg
    22021 aggccgtcct gcggggaatg atcggccccc aacaggtgct tgccttcctc ggcgtcctgt
    22081 tggcgtttca tctgttctac tcctattgct gcctgttgat ctgccgcaag accggcaatc
    22141 cgggaggctt ttgggtatgg gtgccgctgt ttcaattcct tcccctgctg cgggccgccg
    22201 gaatgtcgcg ctggtggttc ctggcctgct ttgtgccctt gctcaatctg gtgccggcca
    22261 ttctatggcc gctaaagatt gccacagcgc ggggcaaaag cgtctggatc ggagtgctcc
    22321 tgctcctgcc ggtcaccaat gtctttgcgt tcctctacct ggccttttcc aacggcgcgc
    22381 ccgcccggga cgaaaaacgg ccggcggcga aaataatgac gctgcaggcc gcctgatcgg
    22441 ccccgagccg cacggattcc cactgtcggc ggcggccgcc ctgacgcggg cgggtcagcc
    22501 gaagcggccc tcgcgaggag ctactcccgc gccttggaat caataatgat cgtgaccggc
    22561 ccgtcgttgg tcaggctgac ctgcatatcc gctccgaatt cgccggactg gaccggccgc
    22621 cccaggtcca gggccagccg ctggaggaac gactcgtaaa gcggcaccgc gatctccggc
    22681 cgggccgaac ggctgtagga cgggcggttg ccctttttcg tgctggcgaa cagggtgaat
    22741 tggctgacca ccaggaggcc gccctggatt tcctgcaccg agcgattcat cacgccgttc
    22801 tcgtcatcaa acacgcgcag ccggacaatc ttgccgctga gccattcgag gtcggcgggc
    22861 gtgtccgcct cctccaccgc cagcagcacc agcaacccct gcgcgatggc gcctttggtg
    22921 acgccggcga tgcgcacgct ggcttccgac acccgttgaa tgacggcgcg catcagccgg
    22981 ttgagaaagc atccagtgga gaaccgcaat cccgggtcac ggttctttca cagcggggga
    23041 gcctttttat gccggtgcgc cgcggacttt ctttcgccgg gcatatccgg ttgctcgggg
    23101 tcggggcggc gggaggtgtc cgcccgggac ggcgccgcgg ggacggggag gaccagatcg
    23161 tagcggggct cgtcgtaact cagcagaatc tcgacgtttt ccaggcgggc ctcgaagatc
    23221 tgctccaccg cccgggccac ctgtcccagg tcgagcgcca tcttttgttc cggcgtccac
    23281 tgcgacggtc cttgatagaa ccagccgctt tgcgtgtcgc gcacaaacac tttcatggct
    23341 tcgtcaggcc gctcgaatct gtttcgccag gttgcgtttc atggttgggc tgaggccccc
    23401 ggcggggctc tccgcgcatc accatatatt cacagaacaa accgcggcgg acaatccgca
    23461 cttctactga agcccaactc gcagaattac ggaacctccc cgctggcgcc ccggggcggg
    23521 agcccgggct tggcgccgga ttcgacttcc cggcaattgc gacaaaggaa ctgccgtccg
    23581 tcaaaatagg ttttgaacgc ccacatccgg caaccgcacc ggtcgcagaa ttcaaagact
    23641 tcccgcgggt ctagcttcac ggcaatccag acccggccct gcgttttgcg caccaaatca
    23701 atgagctctc cgacccgatc cgcttgataa tcaaatactc tggctttgcc gcgttcatcg
    23761 gtgaattccc actcaaaggc ggttcccgtg agatactgcc cgtcatcgtt tacgattaac
    23821 accttgaccg cctcgcgatc attcatggga ggcaatatac tccggcaggg ccaccctggc
    23881 cagttggcag gcagtgcccg gcaagaggca aaaaagggct cgccaacgcg cgcccgttgg
    23941 ctgcatgctc tgcccgccta tgtccgaagc tgcaaagttg atccgcattc cctccctgaa
    24001 aaccgtcgcg gagttccgcg gccacgtggc ttccctggga atagatttgc cctgcgaaga
    24061 tgccatcgcc acgggcgacg cctcgccgct ggcccagccc tgccgggcgg tgcttatcaa
    24121 tggcaaacgc cttggcaacc gctatgccgt ccagccgatg gaaggttggg acgccaccgt
    24181 gacggggggc gttacccccg aagtcctccg gcgctggcag cgcttcggcc aaagcggcgc
    24241 caagctcatc ttcggcggcg aagccatggc cgttcgcccg gacggacggg ccaatcccaa
    24301 ccagctcatc attaacgcgc ggaatcagtc cgacttggcg aagctccggg aagccctgat
    24361 ccgcgcgcac caggagcttt atggccgctc ggaggatctc gtcatcggct ttcaactcac
    24421 ccactcgggc cggttctgca agcccaatga ccattcccgc ctcgaaccgc gggttgcctg
    24481 ccggcacccg ctgctcgacg cgagcttcaa agtcaccagc gacgcgcagg tttggaccga
    24541 cagcgaattg gatcaactgg tcgaagacta cgcggcggcg gcgcgcatcg ccgggaatgt
    24601 cggcgccgat tttgtggaca tcaagcattg ccacggctac ctgctgcacg agcttctggg
    24661 cgccttcacc cggccgggca agtacggcgg gtccttcgag aaccgcaccc gcatgttgcg
    24721 ggacatcgtt gccgccattc gcgccggcgg caaccggatc gaactcgcgg tccgcctgag
    24781 cgccttcgac tttgtgccct tccggcctga ccccgcgctc tcgcgtccag gcaggcccgg
    24841 gcccggggtg ccggtgccat tctcccattg cctgccctat cgttatgcct tcggcgtaaa
    24901 tccgcagaac ccgattacgt atgatctgac cgagaccttt cagtttctgg acctgtgcgc
    24961 cagcctgggc gtcaagatca tcaacctgac ggccgggtcg ccctactaca acccccacat
    25021 ccagcgtccc gccgcctatc cgccatccga cggctaccag ccgcccgaag accccctggt
    25081 cggcgttgcc cggcagatta atgtcgtgcg ccaactcaag gctcgcgcgc cgcaaagcct
    25141 ccttctcgtc ggcaccgcct acagctacct gcaggaatac ctcccgcatg tggcgcaata
    25201 cgtcttgcgc cacgattggg ccgacctcat tggcataggc cggatggtat tgtcgtaccc
    25261 gaccattctg gcggatgcga tagggcgggg gactctccag tcaaaggcta tctgccgcac
    25321 cttcagcgat tgcacgaccg gccctcgcaa cggcctcatt agcggctgct accccctcga
    25381 ccgctattac gccgtccggc ccgaggcgca aaaactgaag gagattaaga aggcttctga
    25441 gcaggcggcc tgactcggcc cgcctccgtt tgacgcaggt tcaactggga gccaattcag
    25501 gcttcagcct cgcggtcgaa ctgtgccaat ttggcacacc gatcacctca taaaacaact
    25561 ggcgtccata ccaatctggg ccaagcatta acgcctcaga gtgtccaatt attggacacc
    25621 ccaaggggtg cctcggcaaa taggcactgt cccagctcaa ccgacccaat ctccaaccac
    25681 ctcaattccc gccattccca gctcatttcc agctaataaa tagccgtgta caaggggtgt
    25741 acaagggatg cccaccggat gttcatcaga ttttccgatg ttcacccggt gggcatccct
    25801 tgtacacccc ttgtacacgg ctatttagta gggggaaagg gtggagatgg acggaaatga
    25861 ggatgttcgt aagggattcg cgggcaggga tggggaggcg aaagggtttc tccatagggt
    25921 gcctttgagg gctgcctttg gagttggggt gtgccaaatt ggcacagttt tagagccggt
    25981 tggaaggggg tggtggcatg gaggttcccg gcgagatttt gggcgggtaa agccggtttg
    26041 gaactggcgc ttgagcggga gccacgcggt gcgcccgccg gtttatccgc ccgtggtccc
    26101 ggctttactt tggttcggtg gcctcgtatt tcttggttca atgcaaagcg cccttccgga
    26161 agcgactgcc gctccgcttt atttcaactt tgcctccgat gtgattgatc ggtgggcccg
    26221 ggagcgaccg gatgcgccgg cgctttggtg tgtggatgcc gcgactggcg aaggccggaa
    26281 attcagcttt cggcaattag cggaactgag ttgccgggcg gccaactgcc tgcgcgccaa
    26341 tggcgtcggg cgcggcgacc gcgttctgct gatgctgccg cgcgtgccgc aatggtggat
    26401 tgccatgctg gggcttatcc ggttgggggc ggtgcccgtg cccgccacgc tgctgctgac
    26461 ggcgcgcgac gtggcttatc ggctgggcag cgcacgcatc cgcgcggcga tcaccagcct
    26521 ggacggagtg ggcaaggtgg gcggctttga gggggtgcgc ttgctggtcg gcggccagcg
    26581 cgcgggctgg gcggatttcg atgacggcgt tcgccaggcc agcccggagt tccgcgccga
    26641 accgacccgc agcgacgatc ccgggattat ctacttcacc agcgcgacga cgggcgaccc
    26701 caagatggtg ctgcacacgc aggccagcta cggactcggc caccgggcaa ccggcgaatt
    26761 gtggctggac ctgaagccgg acgacctcca ttggaacctg tccgacctgg gctggggcaa
    26821 ggccgcgtgg tcaagtttct acggtccgtg gcacatgggc gcctgcgtct ttgcgctgga
    26881 cacccccgga aagttcgatc cggtgttgac gctcgacacg ctggccgcgt ttcccattac
    26941 cacctggtgc gcgccgccga cggcgctgcg gctcatcgtt cggcaggatt tgtctcgctg
    27001 gcgtttcccg cacctgcggc attgcgtcac cgccggcgag ccactcaacc cggaagtgct
    27061 caatcgctgg cgcgaagcga ccggcctgac tctctacgaa gcctacggac agacggaaac
    27121 ggtggttctg attggcaact ttcgttcgct gggccatccc gtgcggccgg gttcgatggg
    27181 caaggccacg ccgggatata gcgtggcgct gctcgatgag catctgcggg aagtgccgga
    27241 tggcaaggag ggcgaggtgg cggtccgggt caaaccgcat cgcccggcgg ggctgttcag
    27301 cgcgtattgg ctcagcccgc gggaaatggc ggcgcagttc cagggcgact ggtatctgac
    27361 cggcgaccgg gccacgcgcg acgccgacgg ctatttctgg tttatcgggc gcaaggatga
    27421 tgtgatcaaa agttccggtt atcgcatcgg gccctttgaa gtggaaagcg cgctgctgga
    27481 gcatccggcg gtgctggagg ccgcggtcgt agggcagccg gatgcgctgc gcgggcagat
    27541 cgtcaaggcc tacgtggtgt tgcagccagg catggagggc agcccggaag tcaaggccga
    27601 gctccagacc cattgcaaaa gcctggtggc ggcgtacaag tacccgcggg agatcgtgtt
    27661 tgtgcgggag ctgcccaaga ccaccagcgg caagacccgg cgggtggagc ttcggcaaat
    27721 cactcccgtt tgattatggc gattggcgat atgcggttta cccaatagat tccaagggtt
    27781 gacgtatgaa atctttaacg gaatgccggc tttatgcgtt tgtggacacg gcctttcttc
    27841 aagggcgagc gcccggggag gtggcgcgag cgctttgcga cggcggcgcg gacttgattc
    27901 agttgcgcgc aaaggaagcg ccggaggcgg aggtttggcg cctggcggaa gccattttgc
    27961 cggtgacgca gcgggcggga atagggctgg tgatcaacga ttaccccgcc atcgcgcggg
    28021 aattgggcgc ggagttctgc catttgggac aggaggattt ctttggcgcg ggctacacgc
    28081 aggtttccca actcccgctg gcagcggagc cggagaagcc accggcgggc gagggcgggg
    28141 gatgccggcc ccgggtaagg gttgggctta gctcggcgtc gccggaccag gcccgccggg
    28201 cgattgccgc cggggcggat tatttggggg tggggccggt gtttcccacc ggcaccaagc
    28261 ccaccgcccg cgcggtgacg ctcgaatatg tgcgctgggc ggcggcgaat atcccggcgc
    28321 cgtggtttgc gattggcgga atcaatttgg agaatgtgga cgaggtgctc gcggcgggag
    28381 cgcggcgcat ctgcgtggtg tccgccatcc tggcggcgcc tgacccggcg gcggtgtgcc
    28441 ggaaatttca ggagcggctt ggccgggcgg cccgccaggg ttgagacact tgggccgtca
    28501 gacgtaaagg gccgcctgga tttcgcgggc catcatcccc atgaacggat agcggcgctc
    28561 cgggtcgcct tccaggcagc gaaggatcac cttttccata gcgatgggga tgctggggtt
    28621 gtgctggcgc ggcgccacga atccggagcg gtcgagctgc cgggccagaa tctcgcccgg
    28681 ggtctcgccg ggaaagggct tctggttggt gagcaattcg taggcggcga cgccatagga
    28741 aaaaatgtca acgcggggtg agatgggttc gccgcgcaat tgttcgggcg ccatataggc
    28801 gggcgtgccg ggattttttc cggagggttt tcggggtttt tccgggatgg gttgggcgag
    28861 gtcgaaatcc accaggcgga cactggcgtt tcgggtgacc agcacgttct cgggtttgaa
    28921 gtccaggtgc atgaaaccgt tgtcgtgcat gtgttcgagg cccgcggcca tatcaatgag
    28981 gatttgggcg acgttctcca ccagcaccgg gtcctggtcg gcgtggagtt gcttgagatt
    29041 cgaggcttcc acgtaatcca tgagcaaata gggctggcct ttgattttgc cgtgttcgac
    29101 gtagccgacg atgccggcgt ggtcgccgat tttggagagg atttccgccc cgcgcaggaa
    29161 gcgccgccgc gcggagaagt caaagcggag gcgcgcgtgc atgcggcgaa gggcgtaggg
    29221 tttttgccgt tcgtcggtcg cgagccagat gtcggccatg ccgccgctgt tgatgagttc
    29281 ttgcaggtag aaccggccga acggcccggg cgcgggcgtt gcgtggactt ccgacctgtt
    29341 tgctttgtta aacactgatt ctacttatca cggcggccgc catttttcca tcgcgaaaac
    29401 ggcggcttgg cgggaaccgg cgtctcgtct atggtggtcc cgcatgcaag ggctccgtgg
    29461 gttcaccgat gccgtgcgcg gctggctcgc cgcggggctg gcgctggttt atccggaggt
    29521 ctgccaggtt tgcggcgagg cgcgggcggg gccgggggat ggctatgtct gcggaggctg
    29581 ccaggcccgg gtgcggttca ttgagccgcc gttctgcgac cgctgcggcc ggccatttga
    29641 aggagacctc accacgcggt ttgaatgcgc ctcttgccgg gagatggagt ggcactttca
    29701 ttcggcgcgt tcggcggtgg cgacccgcgc cccggtgctg gagatcatac ggcgctacaa
    29761 gtataaccgg gcgctgtggt tcgagccatt cctggccggg ttgctcatcc gcgcggcggg
    29821 gccggttttg agcgggcagg cgccggattt gattattccg gtgcccttgc atttcacgaa
    29881 aaagcgtgag cgggagttta accaggccga gagcctggcg gagcggctgg gcgccgcggc
    29941 gcagattccc gtcaacaaac ggctgctccg gcgcgtgctc cccacccgct cgcaaacgcg
    30001 gctgagccgc caggagcggc tggtcaacat gcgcggcgcg tttgccgtgc aggcgggcgc
    30061 gaaactgaac ggcgagcggg ttgtcctggt ggacgatgtg ttcaccacgg gcgccacgac
    30121 caacgcttgc gcccgggcgc tcatcaccgc cggggcgggt gaagtttcgg tctggacagt
    30181 ggcccgggga atttgaataa tgggcactgt cgggtttaat tgcttggaat gatgatagca
    30241 ggcaaactgg aaggacgcga aggcggctcg cccgtggagc cgaggccctg gcgtttcgcc
    30301 gtgtagcgat ggcaacgact tcctttacca agaagacgct gggcgccgag gcgccggagc
    30361 cggccgtcac gccgccctcg ccctccaatg gcaccgcgtt tgtcaagaaa cccaagctcc
    30421 ccagccgcaa atcccgcaag cgggacatcc ccgaagggtt gtggaccaaa tgtccgaaat
    30481 gcggcacgat ggtttttgac aaggagctgg atgaaaacct gaaggtctgc ctgcattgcc
    30541 agcatcactt cccgattggg gcccgcgagc gcattcattc gctggtggaa acctgcacct
    30601 ttgaagagat ggatgcggcg atgacgagcg tggacgtgct gagctttacc ggggtgacgt
    30661 cgtacacgtc caagctggcc agctaccaaa aggccacggg gcttaaggat gcggtgctga
    30721 ccggcatctg caaaatgggg cggcaccggg tggccctggg ggtaatggac ttcagtttcc
    30781 tgggcggctc gatggggtcg gtcgtgggcg aaaagctcac gcgcctgatt gaacgggcga
    30841 ccgaaaagac gctgccgctg atcatcatct cgaccagcgg gggcgcgcgc atgtatgagg
    30901 gcatgttcag cctgatgcag atggccaaga cctgcgccgc gctggcgtac catgcgaagg
    30961 cgcggttgcc gtatatcagc gtgctgacgc atccgacgac ggccggggtg atggccagtt
    31021 acgccagcgt gggcgacctc atcatcgccg agcctgcggc catgattggg ttcgccgggc
    31081 cgcgcgtaat caaggatacc acgcaagcgg aattgccccc gggcttccaa accgcggagt
    31141 ttctgcttga tcacgggctt attgacgcca tcgtgccgcg cacggagatg aagccccggc
    31201 ttattgagta cctggactat ttgacggaag ggcaggggaa cgacgccagt tcgaaatccg
    31261 cggcctgaac ctgggcgagg tcgcactgcg gggttgcggg cgggcgggtg cgtttgagcg
    31321 caagtttgcg cgtgcaattt gcggctggca ggttatcttc actgccgcat gaaaggcata
    31381 attctggctg gcggcgccgg ttcgaggctc tttccgctta ccttggtagc gagcaagcag
    31441 cttcagccag tgtatgacaa acccatggtc tattatcccc tgacgacgct gatagaaagc
    31501 gggattcggg agttctgcct tatttccacg cctcaggact tgccgcgttt ccggcagttg
    31561 ctgggcgacg gcagcgcgtg ggggttgtct atcgagtatc gcgagcagcc caggccgcag
    31621 gggatagccc aggcgtttct tattgccgat acgttcatcg gctccgacgc ggtgacgctg
    31681 attttagggg ataatgtatt ctacggaggc gatggttttt cccgggcttt tgccgaattc
    31741 aagggcgggg cgactatttt tggttatcac gtcaacgacc cggagcgata tggcgtggtg
    31801 gaattcgacg ggaacgggca ggcaatttcc atccaggaaa aaccccggca accccggagc
    31861 aactacgccg tgcccgggct ttacgtttac gacaataacg tggtggcaat tacccgcggc
    31921 cttaaaccat ccgcgcgcgg ggaattggaa ataacggatg tgaacgtgga atatctgcgg
    31981 cgcgggcaac tgcgggtgta ccggctcggc cggggtttcg cctggctgga cgccggcacc
    32041 agcagcagcc tgcatgaggc atccgcttac gtgcagacga tcgagaagcg ggcgggcatc
    32101 aagattggct gtccggagga agcggcgttt catcgcgggt tcctgtcgtt ggcgcggttg
    32161 gaggcgttga cggccaagat gcccaactgc gagtatcgcg attatctggc cgaagtggtg
    32221 gcggaagcga agcggttagg ggcggtggag gcgcaagggc cggcagccgg agttgagacg
    32281 agatgaatgt tttaaagggt gaaattgccg ggttgctgat tatcgaaccc aaagtgcatg
    32341 gcgacgcgcg cgggttcttc ctggaaactt ggaatcgggg ccgctatcgc gaggcgggcc
    32401 tggatgtgga ttttgtgcag gataacgtct cgttttcacg gcggggcacg ctccgggggc
    32461 tgcattttca gaaccccaat ccgcaaggca aattgctcca ggtgctgcaa ggggaggtct
    32521 ttgatgtggc cgtagatttg cggcgcagtt cttccacctt tggaaagtgg caagggctgc
    32581 ttctctctgg cgaaaacaag cggcagttct atgttcctcc cggcttcgct catggttttc
    32641 tcgtgcttag cgagactgct ctgtttcatt ataaatgcac cgggttctat ttgccgaagg
    32701 atgaaatctc cgtttggtgg aatgacccgg acatcgggat taagtggccc ctgcaagagc
    32761 cgttagtgtc ggaacgggac gccaagggac tgcggtttcg ggacctgccg cctgaccggt
    32821 tattcgcata atgaggaggc atttgacgtc tgccggcacc cctgaaaaca cccccggatg
    32881 gccatgaacc cgccacatat aatgcttatc ggcaaggttg gccaggtggg ctgggaactg
    32941 cgccgggcct tggcgccgat ggcccgaatt acctgcgtgg attttcctga cattgacctg
    33001 accagcggag actcgattcg acaatgggtg cgtggcagcc ggcccgacat cgttattaac
    33061 gcggcggcct acactgcggt ggacaaggcc gaatccgagc cggataaggc gatggccatc
    33121 aatggcgtgg ccccgggaat cctggccgag gaagcgaaga ggctcaacgc cctgatcgtt
    33181 cactattcga cggactatgt ctttgatggc agcagcacac agccctacgt cgagaccgat
    33241 gccccggcgc cgctgggagc ttatgggcgc tcgaaactgg ccggggacca ggcggtgcag
    33301 gcgatcggag gcgcttattt gatttttcgg ctgtgctggg tctatggcgc gcgcggacag
    33361 aatttcatgc tcaccatgat gcgccttgcc cgcgagcgcg agaagctgcg cgttgtgagc
    33421 gaccaggtgg gctgccccac gtggtcgcga gtgattgcgg aggcgacggc actggcgttg
    33481 aaggcggcaa ctgcggcgcc ggatcggcag gcgttttcgg gcatttacca cctggcggcc
    33541 gggggcatga ccagttggca tggttttgcg caagccatca tcaatctcat gcctgaggcg
    33601 gaaaagaagt gcgcggagat cgaggccata tcaacggctg agtatcccac gcctgccagg
    33661 cgcccggctt attcggtgct ggcatgcgat aagctcgaac gcacgttcgg tttgcggctg
    33721 ccgcactggg aggaaagttt gaggcaagtg ttggaagtgg gtcattagca gccggggagc
    33781 aaagacatta tgaacttact gattacaggt ggagccggtt tcattggctc gaatcttatc
    33841 catcaggtta tagaccggcc cgaggttgcc cggctaatca atctcgattg ccttacctat
    33901 gccgggcggc ttgagaacct cgtggccgta gccggacacc caaagtatgc cttcgagaag
    33961 gtggacttgc gtgataaggc cgccgtgctg gaggtcgtgc agcggcatgc cgtcactcat
    34021 gtgatgcacc tggcggcgga gtcgcatgtg gatcgttcga ttactggccc gggggatttt
    34081 gtgcatacga atgtggttgg cacctttaac ttgctggagg cctgccgcgc ggcctgggcg
    34141 ggagcgcagg ggagccgccg ttttcaccat atctccaccg acgaagtgta tggcagcctg
    34201 gggccgacgg gtttcttcac cgaggccacg ccctatgctc ccaactcgcc ttattcggcc
    34261 agtaaagccg ccagtgacat gctggtgcgg gcctatcacc atacctatgg cttggagacc
    34321 gtaattacga actgctcgaa caattacggc ccgtatcagt tcccggagaa gctcatcccg
    34381 gtggtcatcc aaagcgtgtt ggcccgcaaa ccggtccccg tttatggcga tggcatgaat
    34441 gtacgcgact ggctttacgt ttgcgaccac gccgaggcgc tgtggacggt gctgacgcgg
    34501 ggaagcagcg gcgaaaccta caacatcggc ggccacaatg aatgggccaa tcttaacatc
    34561 gtacaactga tttgtgatct gattgatgag ctggcgccgc agctcggcgg cggctcgcgc
    34621 aagttaatta cgttcgtcaa ggatcgcccc ggtcacgacc gtcgctatgc gattgatgcg
    34681 accaagatcg aaaaggaact gggctgggtg cccgcgcata ggtttgagga tggcatccgc
    34741 gcgacggtgc ggtggtatct cgatcatcag gagtgggtgc aggcggtcct gaagcattag
    34801 gacgggcagg atgccccggc ggttctgccg gctcaacggt tatgcaacag aaagtccttc
    34861 tccttggcgg caccgggaat atttccaccg cctgtgcggc gcaacttcgc cagcagggac
    34921 acgaaattta tattctcagc cggggccaaa caacagtgcc cccgggctac cagcccctcc
    34981 gggcagaccg caaggacccg gcggctatgc gccacgttct caaaagcgtg cggcccgacg
    35041 tggtgattaa tttcatcggg tatgatctcc ccgaagtgca gattgattgc gaattgttct
    35101 cgggctcggt aagccaatac gttttcatca gttccgcagt cgtatacgcc aaaccgcccg
    35161 cccggttgcc cttgaccgag gacgctcccc tgggcaatcc gtggtgggac tacgcgcaga
    35221 agaaactcgc gtgcgaacaa tggctgcttc agcggcggca ggaaacgggc tacccggtga
    35281 cgattgttcg cccttcccat acctattcca aactttgggt tcccaacgcg gtctccagtg
    35341 gcagctatac gttggctgcg cggttggaac gcgggaaagc ggttttcgtg catgatgacg
    35401 gtgaaagccc ctggacgctg accgccgcca gtgattttgc gattggactc gctggcctgg
    35461 tgggtaaacc ggcggccatt ggggaggctt tccacattac cagtgatgaa gtgctctcct
    35521 ggaaccagat ctatgccgag attgccgcgg ctgtgggggc ggaggcgccg cgcgtggtaa
    35581 aggtgccgac ggacttcatc tgccaggtgg cgccgcaaat gaccggtccg ctcaagggcg
    35641 acaaggccca tcctgggata ttcgacaaca ccaagataaa gcgcctggtc ccggaattcc
    35701 gttgccgcaa atctttccat acgggggttc gtgagtcagt acaatggttg cgcgcgcatc
    35761 ccgagcagca gaacctccgg ccggaattgg atgctttgat tgaaaacgtg attacggcgt
    35821 gggaacggca gggatagcac tgccggggtt ccctcgttcc ttcttccggt gtcgcccaat
    35881 taaaggcatt cgggagcaga agtcagagca aagaaaggtt ggtaaatgac aaagcggatg
    35941 cagctaattt tgctcccccg gcactacgct ttaacttact attggcccat gtgctatgct
    36001 gcctcggttc gaacagaggg aagaccggcg tgagtttttc cgcgctgccg cacgctacgg
    36061 cctgctggcc gtagtgagtt ctattgccgc cctggccggc cgttcgcgaa ggacggagag
    36121 gcagcggtgt ttgaactcgg gcatttgccg ggggtgtagc cattttaccg cgtgcgggct
    36181 gcccgcggcg ctttccgcca aacgagccca gtcaagaggc tgacgtgaac cctgcggaat
    36241 caatcctcac ccggcggcgt ttcctggtag gaaccgcgcg ggctgcgggt cttctcgctg
    36301 cgggaggcag tacggtttca tggctgcgcg ccgccagtcc cgggcgggac gcgaatccct
    36361 tcgcccttga tgtagaacga ttgcagcaca cggatcccaa gcttattcat tacgaacaag
    36421 tcgcccggtt ccaaggctgc gctccggatc cgcgccgcat tgccgttggc ccggatgatc
    36481 gggtgtacat cgctgcggcg aatgccgtga ccatactgga ttgcaacggc agactcatca
    36541 gtgaaattgc ctgtgccgca cccgtacgct gcgtagcggt tgccgccgag gggaccgtgt
    36601 atgtcggggt caaggatcac gtggaaacat tcgatcgcac cggccaacgc ctcgcgacct
    36661 ggccttcctc tggccgacgg acctggctaa ccggcctggc ggcgggggag aaggatgtct
    36721 tcgcggcgga ttcaggcaat cgttgcgtgc tgcgctatga taaggccggc aaactcctcg
    36781 gccgcatcgc tgagcggaac aaagaccgcc accttcccgg gttggtcgtg cccagtcctt
    36841 atttggacgt caagctggcg cctgacggct tgctgcgcgt caacaacacc ggtcgtcatc
    36901 gcgtggaggc ctatacggtg gacggcgacc tggaattctt ctggggccgc ccttccgcgg
    36961 gcattgaagg attttgtggc tgttgcaatc ccgttggcct tgcgctgctg cccgacggcc
    37021 gccatgtgac ctgcgagaaa ggcttgcccc gggtaaaagt ttattcgccc aaaggcgtct
    37081 ttgagtcggt cgttgccggg acggaaacct tttacgaaaa tgccaagacg ggcgcgctca
    37141 aaacccggtc ggacggcctg gtaggcggat tggatgccgc ggtagattct cagcagcgca
    37201 tctacattct tgacctggtc gccaatgacg tccgcatcat gcagcgcaaa gcatgaaccc
    37261 cggcatctca aaaccgtcat cggtcacgcg acgccagttt ctgcgcgacg gggctcgcgc
    37321 ggccggactc gcggggttgg ggggcttgct ggggctggtt gccgaacgaa gcaccgccaa
    37381 gggcacggtt tggcaaatcg atccgcagaa atgcgtgcgt tgcggcaact gcgcccttta
    37441 ttgcgtgctg gagcaatccg cggtgaagtg cgttcacgcc tatgcccttt gcggctactg
    37501 ccagctctgc accggttatt ttgaacccga ccccaacgac ttgaataccg gcgcggagaa
    37561 ttgcctctgc cccacgtccg ccatcaagcg cacctacatc gaggaccctt attacgaata
    37621 caccatagac gaacaacttt gcatcgggtg cgccaagtgc gtggaaggct gccatcgctt
    37681 cggcaacggc tcgctctttt tgcaggtgcg ccatgatcgc tgcctgaact gcaacgaatg
    37741 cgccattgcc aaggtttgcg ccggcgacgc cttccgccgc gtgcccgccg atcagcccta
    37801 tctgctgaaa acggatacct tttgaaatga agatagcagg ccgcaggcgg aagacgaaag
    37861 gagctagggg tcggcgctgg gcattgatcg gggggatggc cttgttgtgg ttgctatccg
    37921 acgtggccag cctggcggag cagcgctttc cgccgccaga ttttgaaggc ggccatcaat
    37981 tgcctgttac cacgacgccg ccggcgcgag ccttgacgtt ggattacctg gatgtcggag
    38041 tgctcgggct aagcctgggg ctggcgatct ggctggtcta tcggcggcgt tcccgcaaag
    38101 ggctgatcgt cctttccatc ttctcgctgc tttattttgg tttttggcgc catggctgcg
    38161 tctgcgccat tggctcgctg caaaatgtcg cgctggccct gggggatcgt tcctacgccc
    38221 tgccgctggc tgcactggcc ttttttgtcc tgccattggc ggtggcaatt ttcgccggca
    38281 ggatcttctg cgcgggggtt tgcccgcatg gcgcactgca agacctggtc ctgctgaagc
    38341 cgctcaaagt gccccccggg ttggaagcgg cgctaagcgt gctgccgttt ctctacctgg
    38401 gggccggcgt tctgttcgcc gccacgggca gcgccttcat tatctgcgag tatgatccct
    38461 ttgtcccagt cttccgctta agcgggcgcg cgctaatggt gctgagcggc gcggccttgc
    38521 tcggcttgag catattcgtg ggccgccctt attgccggtt tttatgccct tacggagccc
    38581 tgctaaagct ggccgccacc gtggcgaaat ggcgcgtgcg ggtcacgcct gacaactgca
    38641 cccaatgccg tttatgcgag gcgtcgtgcc ccttcggcgc catgcgcgag ccgcaagccc
    38701 gccccggcac tcaaacccac gtccgggatc gccggcgcct gggatggttg ctcctgctcc
    38761 tgccactgct gctggttggc ggcggctggc tcgggcaccg cttcagcacg cccgcttcgc
    38821 ggctgcatcc gacggtcagt ctggctgacc tgctcatacg cgcgcaggga agccctccga
    38881 agattggcgc gcttacgccg gacgaccttg cgcttgaacg cgccaggcag gcgccgcagg
    38941 aaatcctggc cgccgccgct gccattcaac ggcgatttgc catcggcggc tggattttcg
    39001 gggcctgggt tggattggta ttcggggtga aattgatcag tgtctcagtt caccgcccgc
    39061 gaactgatta cgagcctgac tcgggcgatt gtttcgcctg tgcgcgttgc ttcgagtatt
    39121 gccccaatga actggttcgg cgcggtgtca ttccagctac aacgctcacc gccgcggccc
    39181 cggaaatttc aggcccgccc ggattggacc ggagctgaat gctgacccgc cttttccaag
    39241 tcgaagcggc cagtcccccg cgccaatctg gcggggaaac cgtgttgctg gcttggcggc
    39301 tgacggcgtg ggtggccggc gttttctcct tgctggtcgg catcactttg ctggtcgaac
    39361 acacaagggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcccgcgcac
    39481 gatgggcggt ggcggcatgc ggtatcgttg tgggaagtgg cctttttgtt ctaagccgcg
    39541 cccttggccc gaacctcccc acgccagcgg cagagttgga taaactgctc gccacccgcc
    39601 cggaggaatc gtcttctccg gggcatccgc aggcagccga tgccgcctcg ctcgcggaac
    39661 ttagacagaa ctggccgcgc tttctcggtg cggacggcaa cagcttctcg ccatccacaa
    39721 acgcgcccgc aagctggaat ccccagaccg gcgcggggat agcctggaag gttccaacgc
    39781 ccacccgggg ctttggctcg ccgatcgtct gggccggcca ggtttttctt tccggtggtg
    39841 atgcgcgcca acgcgaggtt atctgtctcg acgcccaaac cggcaagatt ctctggcagc
    39901 agcccgtggc cagtgtttcc gaccgcccgg accgcacgga ggtgccggat tccaccggct
    39961 atgccgctcc gaccatggcc acggacgggc gccgcgtgta tgcgtttttt gccaacggag
    40021 actgtgccgc ctttacgttg gcaggccagc cggcctgggc cagaaacttc ggcccgctta
    40081 aaaatcccta cggccatgcc acgtctctcg ccacctggcg cgaccgcttg atcctgcaac
    40141 tcgaccaggg agagagcgag gacggcaact ccaaactgta tgccctggac gcgcgcaccg
    40201 gccagactgt ctggcagcgc ccgcgcaaaa ccggcgccac ctgggcgacc ccgaacgtga
    40261 tagacgcgga gggcaaggcg caggtgatcg cgttgtcggt tccgtgggtg attgcctacg
    40321 cggcggcgga cggcgcggaa gtttggcggg cggagtgcct gaacggggaa atcgccccct
    40381 cgccggcctg cgcgggcggc ctcctgttcg tagccagccc ttcggaaaag ttgctggcca
    40441 ttcgcccgtc agggcagggg gacatcaccc aaacccatct cctttggacc tgggaggaca
    40501 gcatccccga tgtcaccagc cccgccagcg acggcgaact ggttttcatt ctcaccacct
    40561 cgggcatgtt gacctgcctc gacgcccggg acggcaagaa acagtgggag catgacttgg
    40621 aaatggaatg ccacgcctcg ccggccctgg ccgcgggccg cctttacctt tttgggcaaa
    40681 agggcaccgc ggtcgtagtc gcggcggcgc gggagttccg ggaattgttc cgcacggaga
    40741 tgggagacgc ctttcatgcc agccccgcgt tcgttcagga ccggatgttt ctgcggggga
    40801 tgaccaacgt gtggtgcgtg cgcacgatta acgaagctgg gaaggagggg gcgcaaccat
    40861 gaacccggcg cgcactgaac ccggcgtcgg gccggtggat ctccaatacg tagatgccgc
    40921 catccaacgc attggccgcg cgcccgatgc ggtcatcccc ttgctccagg ccattcagga
    40981 tcactatggc tacctgccgg aagccgcgtt gcggcgggtc tgcgcggcca cgcaaatcac
    41041 gccggcggga atcaatggcg tggccacgtt ctacgacatg ttccggcatc gccccggcgg
    41101 caagcatatt gtccaggtat gccacggcac ggcctgccac gtgggcggtt ccgagcgggt
    41161 cgaagaggcg ctccgccgcc acctggcgat accggaaggg tcggatacgg acgccgcgcg
    41221 gcgcttcacc ctcgagcggg tggcctgtct cggctgctgc acccttgccc ccgtcgtgcg
    41281 catcgagaag gaaaccattg gctatgccgc ggcggaaaag gttcccgaga ccatcgcggg
    41341 attcctcgcc aaccggcagg aagccgctgc caccccaacg gttgcgactc ccgcgccgcg
    41401 gaccggcgcc gctgaaatca gggtctgcct cgattcgtgc tgcctggcga aggggacgga
    41461 tcactcgttc cgggccttgc agcagagcct cgcaaagcac ggcgcaaacg ccaccctgaa
    41521 gcgggtcggc tgtgtggggg cctgctatcg cacgccgatg gtcgaaatcg cgattcccgg
    41581 ccagcccagc atcatctatg agggggtgac cccggagcag gccggcaggc tggtggaaac
    41641 tcacttccgg ccggcggggc tgacgccgcg tatttcgcag ttatacaccc ggatcgtgga
    41701 cggcctgctc gtggaagatg cggacgccat cccgccgccc ggccgcccgg cggatgccaa
    41761 agacggcgcc ggcgaccagg cattctttcg gcggcaggtc catattgcca tggagcatta
    41821 tggccgcctc gacccgctgg atctggacga atacctggcg cacgatggct ttgcggcttt
    41881 gcgcaagtgc ctgggaatcc ctccccccga ggaaaatcgg cgggtagacg gcggccccaa
    41941 ttccgcggcg gtctccccgg agcaaatcat cacctccctg gaacgctccg gtttgcgcgg
    42001 gcgcggcggc gcgggctttc ccaccggcca gaaatggcgc atggttcgcc agcaacccgg
    42061 cgcgaccaaa tacgtcatct gcaacggcga tgaaggcgac cccggcgcat tcatggatcg
    42121 gatgatcctg gaatccttct cctaccgggt cattgaagga ctggccattg cggccgtggc
    42181 catcggcgcc cacgaagcca ttttctacat ccggcacgag tattcccagg ccttgcagcg
    42241 cgtccgggcc gcgctggcgg aatgcgaacg gcgcgggtgg atcgggccgc gcttgctggg
    42301 cagcgattat ccgctgcgca tcaccatcaa ggagggagcg ggcgcgtttg tttgcggaga
    42361 agagacggct ttgattgcct ccgtcgaggg gcgccgcggc atgccgcgct tgcgcccgcc
    42421 gtttccggct caagccggcc tgtggggcaa gcccaccctc atcaacaacg tcgaaaccct
    42481 cgccatggtg ccgtggatta tccgccgcgg cgcggaagcg tttgccgcca tcggaaccgc
    42541 cgccagcaag ggcaccaagg tattctccct cgcgggcaaa gtccggcgcg ccggcctgat
    42601 cgaaatcccc atgggcacga ccctccgcca gattgtcgag gaaatcggcg gcgggattgc
    42661 ggacggccgg accttcaaag gagtccagat cggcgggccc tccggtggct gcgttccggc
    42721 gcgcctggcg gatacgccgg tggactatga gtcgctgcgc gacatcggcg ccatcatggg
    42781 ctccggcggc atggtggtgc tcgatgacac cgcctgcatg gtggacctcg cgcggtattt
    42841 cctgcaattc acgcaaaatc agtcctgcgg caaatgcacc ttctgccgca ttggcaccaa
    42901 gcggatgctg gaactgctcg aactcttctg cgccggccag gccacccgca aacacctgcg
    42961 ggagctggag caactggcgc cccaggtatc cgcgggaagc ctttgcggcc tcggcaaaac
    43021 cgcccccaac ccggtgctga ccacgctgcg ctatttccgc gacgaatacg aggcgcacct
    43081 gcagggccgc tgccccgccg gccggtgcaa ggcgctgatc aaatatcagg tgggggataa
    43141 ttgcaccggc tgcacccttt gcgcgcagca ttgtccggcc gacgccatcc ccatgacccc
    43201 gtatgagcgg catgtgattg acctgcaaaa gtgcacgcgc tgcgattcct gccggcaggt
    43261 gtgcccctac ggggcggtgg agatcgtatg agcgccatcg ccatccacat tgatgggcgg
    43321 ccggtgtccg tgccgccggg caccacgatt ctgggcgccg cgcgcaaact gggcatcgcc
    43381 atcccaaccc tctgccacgt cgagggcttc gagccgtccg cttcctgctt tctgtgcgcg
    43441 gtgcagatcg aaggacgccc caatctttgg ccgtcctgcg ccacgccggt agcccccgga
    43501 atggcggtga tcacgaattc ggcggaggtc cgggccgccc gcaagaccgc gctggaattg
    43561 ctgctttccg accacgcggg cgattgtgtg gccccgtgtc ataccggctg cccggcgggg
    43621 ctggacatcc ccgggttcat cgcccgcatt gcggccggtg ataacgccgc ctcggcgcgc
    43681 ctggtgaccg acgggcttac cctgcccgcc tcgctggggc gggtatgccc gcggctgtgc
    43741 gaacagcatt gccggcaatg cgatgtgacg gaggcgctct ccatccgcaa cctgcatcgc
    43801 ttcagcgccg attaccagcg ccaggcaaaa tcaccgcccc cgcccgctga ccctccggcg
    43861 cctcccaccg gccggcgggt ggccatcgtc ggagccggcc cggcggggtt ggccgccgcg
    43921 catcacctgc tgggccaggg acacgcggtg gcgctgttcg atgcccattc gcaacccggc
    43981 gggatgctgc gctatggcat cccggccttt cgtttgcccc ggcaggtgct cgatgcggaa
    44041 atccaagcga tccgcgccct gggcgccgaa tttcacctgg gccggcggtt gggccgcgac
    44101 ttggccctgg acaacctgcg gcgcgacttc gatgccgtat tcctggccat cggcgcgcaa
    44161 ggctcccgca gcctggggtg cccgggtgaa gagcttgcca cctccgccct ggagttcctg
    44221 gaaacgatcg cggacggctc cccccccgcg attgacggag atgtcattgt ggtgggcggc
    44281 ggcaacaccg cgatggacgc cagccgttgc gccgtgcgcc tgggcgcccg ctcggtgcgg
    44341 gtgctttacc gccgcacgcg ccgcgagatg ccgtgcctca tggaagaagt ggaggcggcg
    44401 gaggccgagg gggtccaggt cgaattcctc gtcgcccccg cccgattgga gcgcaaggag
    44461 ggaaagctga acctcatctg tcagcgaatg gaattggggc cacccgatgc cagcggtcgc
    44521 gcgcggccgg tgcccgtccc cggctcggaa ttcgccttgc ccgccacttg cgtcatcgcc
    44581 gccatcggcc agcacgtcaa gaccgacggc ctggatcatt cgcaactcgc gctgtcgaaa
    44641 tggggcattg ccgccgaccc taaaaccctg gccacgaatt tgcccggcgt attcgccggc
    44701 ggcgatgccg tttccggccc ggatttggcc gtgcgggcgg tagcggccgg caaacttgcc
    44761 gccgtggcca tccaccaata cctggccggg caaaaagtgg tcggcacgcc ccccctggcc
    44821 agcgtcatga tgggcaagct gagtgaagca gagctggcgg tgctcctgcg cgacatcgag
    44881 caggcgccgc gcgcgcccat gccgcatctg cccctggaag ctcggcggac tacctttgac
    44941 gaggtggaac tgggcttctc cctcgaaacg gctacgcgcg agtcacggcg ctgcctgggg
    45001 tgcggttgcg gcaaggccgt tccctgccgc ctccgccagg tcgccaccga gtacggcgtg
    45061 gatccccagc gtttcgttgg cgagcggcgc ctctttacgc gcgacacctc tcacccggac
    45121 atcatcttcg agccgggcaa gtgcatcctt tgcggcgcct gcctgcaagt agccgcccag
    45181 gcgggtgaag aactggggct atccttcgcc ggacgcggct tccaggtgtc ggtggccccc
    45241 cccttccatc gcccggtggc ggaagccctg cgtcaatcgg gtcgccgtgc cgccgaagtc
    45301 tgtcccacgg gggcgattat gctcaaaagc gccgggtgcg ccggttgccg tctggcctga
    45361 ccaccctagc gcaactcgct gggagcgagc aggagcacga caaagcaccg atcttggcct
    45421 ttttaagcca cgccggactg tccaattatt ggacactttc cgctttccaa cttcataacc
    45481 cacgtcgagc accggtttcg acttaacttc aatcattcca ccatcatcca tagcagcctc
    45541 ctgcccgtgt acaaggggtg tacaacggat gctcatcggg attacatcgg tttttctgtt
    45601 gttcatccgg tgagcatccg atgtacaccc cttgtacacg gcgtcttggg agggtggcag
    45661 gacgccattt gggggttgcg gttggcgggg gattgcggga tggggtttcg cgctgttcgc
    45721 cgagttgagg gtgtgtttcg gcaagccggt ttgtcagctc agattgagag gcgcggccat
    45781 cagcgcgtcc ttgcttcctc cccgccggaa ttgctaatct tccagcatgt ctcgcaaggc
    45841 gtcagcaagc tcagactcct cccgtgggtt tagcgacatc atcgggatcg tgctgatgtg
    45901 tctcgctatt ttgctgatgg tggcgctgct ctcctaccat ccgcgtgacg tatcggccaa
    45961 tgcggtgccg cataaccagc cggcgctgaa ctggatcggc ccatttgggg cgtggatggc
    46021 gtatggctgt ttcctgaccc ttggggcggc ggcttttgtg ctgccggtgg tgttgttttt
    46081 cgtcggcgtg gggtgctttt tcgaaatgtt cgcctacttg cgccgccgct gggtctgggc
    46141 ggtggtgctg ttgatctgct gcatggggct gctggacatc tatcggcatt acctgggcgg
    46201 cctggagcgg aacctgggcg tggtggcggg cggcattctc gggcgcaacc tcaaccagca
    46261 tctcttcagc gagtgttttg gcgccgtggg ggccacgatc attttcctca tgctctattt
    46321 tatcagcctc ctcttcctga cgaatttcca gttgggcgac tgggtccggg ccttatggag
    46381 ccggcgtccc cgggcggggg gcaaagcggt cactgaggag caggcgctgg aacagcgggc
    46441 gcgggagttg cagcaacagg ctcgcaagct gcaggaggaa gtggatcgcg ccgggctggg
    46501 cgcggatatg cagccggtgc ccaagccgac cgtgcgcgac ctgagcgtgc cggaggccaa
    46561 taaggctgcg cggggccgga aagctgccgc ggccagtccc gctccggagc cggctccggc
    46621 agatgagggc gaagtcatcc cggcgcatga agtggccgcc gccaccacgg ccgaggtttt
    46681 gggccagccc ccggaaccgg ttgcggattc ggccgaggcc cgggcggccg gggacaaagc
    46741 ggaaaccgcc gcggcggaga aatccgaggg cgccgacaag ccgtccgagc cccggcgcga
    46801 ccaggaagtg gttattcacg gcctgccctc cgcggcgtcc gccaggcccc ggaagaagcc
    46861 caagccgatc gccgtcgccg ccgcgcccgt gatcggtaac taccagttgc cgcccctgga
    46921 tttcctgcag catcccgatc ccaacctgaa gcccaccgaa tccaaggagg agctgctggc
    46981 caatgcccgg ttaatgcagc agacgctggc ccagtttgaa atccccgtcg agttgggcga
    47041 tatcaccaag ggccccacca tcacccgcta cgagttgcat cccgcgcccg gcgtcaagct
    47101 ggagaagatt accgcgctca ataacaatct ggctgccgca ctcaaggccg agcgcatcca
    47161 tattctggcg ccggtgcccg gcaagagttc cgtcggcatc gaggtgccca atccggtcaa
    47221 gaccgccgta attatccgcg acctgctgga atcggaggaa tggcgcaaca ccaaagcccg
    47281 catcccgctg gcgctgggca aggacgttta tggccacccg attatcagtg acctttcgga
    47341 gatgccgcat ttgctcatcg ccgggagcac gggcgcgggc aaatccgttt gcatcaacgc
    47401 catcatcacc tcgttgctct atcggtttcc gcccgaccgg ctgcgcttcg tgatgattga
    47461 tccgaaggtg gtggagttgc agcagtacaa cctgctgccg cacctggtgg tcccggtggt
    47521 caccgacccc aagaaggtca ttctggccct gcgctgggtg gtgaacgaga tggaaaagcg
    47581 ctatcagatt ttcgcgcggg tgggcgtgcg caacatcctg tccttcaatt cgcggccccg
    47641 gaacaagccg ctgccgccgc gcgagcccga gctggagctc ctggcccgga aggaaaaggt
    47701 ggagccgggc gccgacggct ttgccgtgga ggtggacgag gccatcgtag tcccgcgcga
    47761 ggaggacatc gtcatcccgg agaagctcag ctacatcgtg gtgatcattg acgaactggc
    47821 tgacctcatg ctggtcgcgc cggcggacgt ggaaatggcc atcgcgcgta tcacgcagat
    47881 ggcgcgcgcg gcgggcatcc actgcatcgt cgccacccag cggccgagcg tggacgtcat
    47941 caccggcgtc atcaaagcca acattcccgc gcgcatcgcg tttcaggtgg cggccaaggt
    48001 ggattcgcgg acgattcttg atgcgatggg cgcggataaa ctgctcggca aaggggacat
    48061 gctgttcctg ccgcccgggt cggccaaact catccgggcc cagggctcgc tcattaccga
    48121 tcaggagatc cagaacatcg tggagttcat cgcgaagcag ggcaagccga gctatgaact
    48181 cgagattcac cagcagcttt cccgcgctga caacggcagc gggagcgggt ccggcattga
    48241 cgaggacgaa gagatcatcc aggactgcat cgaagtcatc cggagcgagc agaaggccag
    48301 cgtctccctc ctccagcgcc gcctgcgcct gggctatggc cgcgccgcgc gcatcatgga
    48361 tgagttggag aaccgcggca ttgtcggccc cagcaagggc gccgagccgc gggatattct
    48421 cattgacctg gatggcggcg gggcggacgg gcggccaccg cagaatgagg agcctccgcc
    48481 cccggcttga ccgcgggccg cgcggcgttt ccgcgcttac actatgaacc ggggttcatg
    48541 caccgggcct gcgcggtgcc ggcggagcag gtcaataaac tttgccaggc cgcgcttttc
    48601 atccgcgccg atatggtagt ggatatgcca tcccaggtaa tccttgcgga aatcgctggt
    48661 gtattccgtg cgggtgcgga tgatggagtc gagcgtatcc attccgaagt cgtgggcttc
    48721 gcggagcaaa cgccgcagcc gggcattttc gacgccgcgg cgcagggccc agacggcgaa
    48781 gacgaagggc aggccggtca tctcatacca ggcggccccg agatcccaaa tatcgtgctg
    48841 atgcgggccc agggccaggt cgagcgccgt gtcgccgatg agcagggcgt agtcgggcag
    48901 cgcggccaga tcataaccgg gcaggggttt gaactcgggc gtaatgccgc gctcggccag
    48961 caggattcgc agcaattgca cgctggtcag cgaggcggga tcacaaaaga tttccgccgc
    49021 ttcgtccagg ggcttgcggt gggccagcaa gacgcttttg acttcgccca acgcggcaat
    49081 gccaatgccg tccaggatgt cgtagcggtc atggaacagg acttcgacca cgctgaccag
    49141 tgccgcatcc aattcgtcgc gttgcagcat ttcggccagc cgggcgggcg gggcatagat
    49201 aatctcctcc tccaggccgc gcgtgagcgg gacggtgttg aggaagccgg agtttcccac
    49261 gcgaaagggc gccagcgact gttcgaggct gcgctcgcgt tgcaggtcca cggcgcgttg
    49321 ctcgcgctcg aagcggtggg cggcctcggc cggcgtttct accggcgcca tgcggagccg
    49381 gggagggtcg tcgggggagt tggtcatgcg cttgccaggt tggcttcccg caccgtggcg
    49441 ggcgcattgc cagggtccgc ttccggcggc gtatcctccc agacgcggac gggctcatag
    49501 aaggtatcgc gctgcacagg gatgcagccc gcctcgcgaa tggcctggat catggcggtt
    49561 tcgctttgca gttgcggggt ctgcgcgccc gcctggtgaa agatatgttc ttcgaagatg
    49621 gtgccgtgga gatcgtcggc cccgtagccc agcgccactt gcgccagctt caagcccagg
    49681 cccacccagt aggcggtgat gtgatcgaag ttatccaggt aaatccggct gaccgcaatg
    49741 gttcgcaggg tgtcaaaccc cgtgggcggg tttgccaccg gaatgccgtt gttttccgga
    49801 tggtagggca gcgggacgaa acccgtgaat ccgtgtgttt cctcctgcaa gccgcgcaac
    49861 tggcgcaaat gatccacccg atcggccagc gattcaacat gcccatacag catcgtgcag
    49921 gtgctgcgcc cgcccagacg atgccacgtg cgatggactt ccaggtattc ttccgccgat
    49981 tccttgccgc gcgcgatggc cgagcggact tccgggcgga aaatctccgc gccgccgccg
    50041 gtcaacgagt ccagcccggc ttctttcagg gccgcaaggg tctcggccac gggtttccgg
    50101 gccagccagg ccaggtgcag gacttcaatc gcggtaaagc atttcaactg cagacgcgcg
    50161 tccagcgccc gcagcgcccg cagcatatcc gtatagtagc tgaacggcag ggaggggtgc
    50221 agccccccga cgatgtgcaa ttcggtgatg ccgaactgca acgcttcgcg cgctttcgcg
    50281 acgatttcct cgaggctgag ttgaaagcca ttcgcgtccc gcttcttgcg ggcgaaggag
    50341 cagaactggc agctcaggat gcagtagttc gagtaattga tgtaacggtt caggatgtag
    50401 ctggcccggc ggccgacctt gcgctggcgg gccaggctgg cgatggcgcc cagcgcgttc
    50461 aggtctttgc tttcaaaaag gcggagcgcg tcggcttcgg aaatccgctg gccggcggcg
    50521 accttgtcat acaggtcgcg cagttcgctg cgctgaacaa agaaatgcac tcattaccat
    50581 taccagaagc ggcggctttg gcaaccgggt ttcaaggaag tttgggttgc cggcgaaagt
    50641 gttgatccgc aaagtccaga aacacccgcc aatcagccgc ggtcatggag tgtttgcccg
    50701 cgcgaatata gtatcccagg ggcgaggcga tcagtttgcc ggggggcggc atttccgtgg
    50761 cggccagccc tggcgatccc aggaagcgat aaacgggatc cgccgcttgc agcacctcga
    50821 actggccggc tggatttgcc cactgatctt cctccgccgc cgaaaagagc accggccggg
    50881 gggcgcacag cgcgaccagg gcgttctggt caaatggcag ccgctccggc gcgtcgttga
    50941 attgcttgaa gcgggcgtta aaccagtgtg ggaaccgggt gttaatggct ttcaccgatt
    51001 cgccgatttt gccccggctg ggggcggtgc cgccacatcc ggcctggtgc gggaaggcca
    51061 tcgcgatgcg ctcatcaaac gccgccgcca gcaacgcggt cttgccattg cgcgagtgcc
    51121 ccagcgtggc gatgcgcttt gcgtctatgt cccggtcggt gaccaggtag tccacgcacc
    51181 ggtggaagcc ccaggcccag gcggcaatgg ttccgcggtc ggcgggggtg ttgcggcggg
    51241 gatcgttccc ggccagccag gcgtacagtc ccaggctggc gtcgggccga tccgcatcca
    51301 ggtccgcgtt gcaaaacgtc gccagggcgt atccgcggct gacgatctcc gccaggggcc
    51361 agtcatcgct ctgcgtgccg cgcgcggcat cggtcgcgac gttgttggtg catccgcggc
    51421 agttgccgcg cagccagccg cgcgtgagtg gaatgcgggg gtcggaactg atcgcctggt
    51481 tgccccagaa attaagggcc agaaagaccg gcaccggccc ggggcggtgg ttgggcagga
    51541 tcatcatgag gtcaatgcgc ggggcctgcg gatcgtgggg cgccacttcc agcgttatca
    51601 ggcgcagcgt ggcctggccg cccaggaaat cgcggtgctc gccggtcacc gtggctttca
    51661 actgcgcagg tttcggcggg atgggcccat acatgtaata ggcgaacagt tcctttaatt
    51721 ccgggcgtcg ctgccgaaac cattccgaac gggatttgat cctgtgcccg tccattttta
    51781 tcagcggatc aggcagcgta gcctgcgccg gcaggcgggc ggcatccgga aacgcgcgtg
    51841 gcgggagcga atggctggcg cagccgcagg cggccgccag agccaggggg gcgaatcggt
    51901 gtgtgaaggc agtcatgttg cggggaaagc tacgcgctgc ctcgcgattt gtcaggcgga
    51961 ttccgagcgg cggccttcgg gcttgacgaa gcgcccgaca gcggttgttt tatggccagt
    52021 gctaaaatta tgatgaagac ttcattgttt gttctgcttg gaatcctggc cttgcccgca
    52081 ctggcccaac cggccaggcc ggttgtgatc gcgcatcgcg gtgccagcgg ctatctgccg
    52141 gagcacaccc tcgagtccaa agtgctggcc catgcgatgg gggcggactt cctggagcag
    52201 gacgtcgcgc tgaccaagga tgatgtcccc atagtgatcc atgatattta catagagacg
    52261 atcagcgatg tcgcgcagcg atttcccgag cgcaagcggg ccaacgggca ttactatgcc
    52321 ctggatttca cactggcgga gattaagcaa ttgcgcgctc aggaacgcgt caatccccgg
    52381 accgggcagc cggtctatcc gaaccggttt ccgccgggta aatcctcttt ccagattgca
    52441 acgctggagg aggaattaca gctcatccag gggctcaata agaccagccg ccgcgtggtg
    52501 ggcatttacc cggaaatcaa acagccagcc tggcatcgca aggaaggcca tgacatcagc
    52561 cgcattgtgc tgcccatcct gcggcgctac ggctatgcga ccaagcaaga tccctgctgg
    52621 gtgcagtgct tcgaactgga cgaggtgaag cggatccgca ccgaattggg ctgggaagga
    52681 cgcctgctcc tgttgctggc gatgaaccgg aagcatccgg acggcacaag cgcggaagcc
    52741 tggttcacgc ccgccggcat gatggaggtg gccaaagtgg ccgacggcat cggccccgaa
    52801 ctgggcgcaa ttgtatccgg ctcatccaaa gccacgcgca aggtcactga ttttgtaaaa
    52861 catgcccatg cggcaaaact gattgtgcat ccctacaccc tgcgagccga tgacttgccg
    52921 aagtttgccg aatccatgga tgatgccctg gaggtgctct ttgccgaagc cgggctggac
    52981 ggttttttta ccgactttcc cgatttgccg gtgaagtggc tcaccgcgcg cggtttgcgc
    53041 tgattcgcca gacgaaatcg gcgcacgggg cgatagggtt ttgtctcgcg cgtgacgcaa
    53101 tccagccgcg ctccggaatg tcatgccggt cggttagcta ccggcgatga ggctgtggcg
    53161 ggcgcttttc tttgccgggc tcacatgagc ttcttgagct tcgggttcgc cgccagccgc
    53221 ttgaccagtt ctttaacttt ctgggcctgg cttttgtgcg ccagcagggt ggcgtcgtcc
    53281 gtctgaacga ggattgaatc gcgcaggcca accaccgcga tgggggtgcg ccgcgcttta
    53341 ctgcgggcgt cgaagatgat gttccgcgcc ccgtcaacgt gaatgaaatc cgccacggcg
    53401 cagttgccct ccgggtcggc cttgaggtgc cggcccagcg cagtccagga acccaaatca
    53461 tcccaggcaa aggcgccgtc ggccacaatg acgttttggg cgcgctccat cagcgcgtaa
    53521 tcaatcgaga ctttcttgag ctcagggtat tccttcgcga gcaccgcgtt cagccgcgcg
    53581 ggttgagtgg ccaccttgaa ccagcgctgg caagccgcgt gcatctccgg ctggtgcttt
    53641 tgcaagcctt ccgtaatggt gacgaacgac cagatgaaca tgcccgcatt ccaacggtat
    53701 tggccgctgt tgacatactc cagcgccttg tcgaaggggg gcttttccac aaactgctcg
    53761 gctcgataaa aggcggtttt ataggatttc acccccaccg ggggcggcag tttttccccg
    53821 acgcggatat agccatagcc cgtggcgggc tccgtgggtt tgatgccaat ggtgatgatc
    53881 gcctgtccgc gccctgccag atcaaacgca tctcccagga cctgctgaaa ctttttctct
    53941 tccgggatga cgtgatcggc cgggagcacg gccatgactc ccgtcgttga gcgcgctccc
    54001 accagcgccg cgcccagcgt gacggctgcg gttgtatccc ggccgacagg ctcggcgatg
    54061 acgtttcctt tgggcagctt cggcagttgc tggcgcaccg cgggcgcctg aaccgcgttg
    54121 gtgatgacca gaatattctt aagcggcacc agcgccagca cgcggtcaac tgcttgctgc
    54181 agaaaggagc ggccgcccag cagggtgagg agctgtttgg gcattcgctc gcggctgacc
    54241 ggccagaagc gctcgccccg gccgcctgcc ataatgatta cataccgttc gttaatatcg
    54301 ggcttggctt tcataattgt tttacggcgc gcaggagcgg ccgcgtaaag ccgctgaccc
    54361 gctcgattaa atccggcgct ttaccgtgca gtgcgacttg gttcacgatg gcgctgccca
    54421 cgacgaccgc ctcggcgttt tgcgccacga gcctggcctg ttcgggcgtc gaaatgccaa
    54481 atccgacagc gatgggcagg gtggtatggg cgcgaatctt cgcgatcatt ggcgtaatcg
    54541 tatcggccac ctgcgattgc atgccagtga cgccttcgcg cgacacgtag tagatgaagc
    54601 cggccgcccg cttgacaatc atttcaatgc ggttctcggg ggtggtgggt gccaccaggt
    54661 agatgttgca cagtccggcg gcgcgcattt tcgcttcata gtcagcgcct tcctccggag
    54721 gcaaatcaag caccagcagg ccatccacgc cggccccggc tgcatcccgt ataaagctct
    54781 caacgccctg ccggcagatg agattgaagt aaacgtaaag cacgatgggg atttgcgaat
    54841 cccgccggat agcagcgacc gtcgccaaaa gacgggatgg tgtggtgccc gaatccaggc
    54901 cgcgctgggc ggcgagctgg ttgacgactc cgtccgccag cggatcgctg aagggcactc
    54961 ccagttccag cacatccaca ccgagttgat ccagcgccag cgccagttgg cgggtcgctt
    55021 cgaggttggg atcgcccgcg ccgagataaa caatgaaacc cttctgacgc tcccggttca
    55081 agcgggcgaa tcgttcaaca atgcggttca tccgtgcctc agtttcaggt tttgcgtccg
    55141 gcgtttcaag ctgcaagttg cggagttatc ctccttttcg gttcgtgacg aactgctgcg
    55201 gttggaggaa cttcagggcg atggcggatc gccggttctg tgattagacc cgttttgtca
    55261 gcgacgctga cttatctgtg gtggcccata tcacccgttt tggaggcggg ttcgagagcc
    55321 ggggccggta acagtttgcg tggcttgccg cctggcctgt aattgcgctg attgcgggcc
    55381 ggtgtgcgaa aagtaaaatt aactcagtcc ccccaatccg ctgcaggtac agatcaggtt
    55441 gcggtcgccg tggacattat ctatgcggcc gacggcgggc cagaacttgt ggtcatacag
    55501 ccacttggcg gggaaagcgg cttgctggcg ggagtaggaa tgatcccatg attccaccgc
    55561 gagctgatcg gcagtgtgag gggcgttctt tagcggattg ttatgtttgt cggactggcc
    55621 ggattggatg gcggtgattt cggcgtgaat atggatcagg gcgtcgcaga aacgatcgag
    55681 ctcttccttg gactcgcttt cagtgggttc gaccatcatt gtgccgggca cgggccagga
    55741 gatcgtcggg gcgtggaacc cgtaatccat tagccgcttg gcgacatcct cgacggtgac
    55801 gtccttgaac tggcgcaggt cgaggatgca ttcatgggcg acgaggctgg cggggccgcg
    55861 atagagaatg gggaagtagt tttgcaggcg ccgggcgata taattggcgt taaggatggc
    55921 gtattgggtc gcctcggtca gcccggcccc acccatcgcg gcaatataga cccacgggat
    55981 tacgaggacg ctggcgctgc cccagggcgc ggcagctacg gcgccgatcg ggttttcgcc
    56041 gccgagattg acgaccccgt gccccggcag gaactccact aaatgctcag ccaccccgat
    56101 gggccccatg cccggaccgc cgccgccatg aggaatggcg aaggttttat gcagattcaa
    56161 gtggcaaacg tcggccccga tatcgccggg gcggcaaaga ccgacctggg cgttgaggtt
    56221 ggccccgtcc atatagacct ggccgccatg ggcgtggatg atttcgcaga cctcgcggat
    56281 ggcgggctca aatacgccat gcgtcgaggg ataggtcacc attaccgctg ccaggttggc
    56341 ctggtgcgct tcggctttgg cccgcaggtc ggcgaggtca atgttgcctt cgtggtcgca
    56401 agccaccgct acgaccttta gcccggccat gacggcactg gcggggttgg tgccgtgggc
    56461 tgatgtcgga atgaggcaga tgttgcggtg accttcaccg cggctggcat gccaggcgcg
    56521 gatgacaagc aagccggtat attcaccttg cgaccctgca ttgggctgca aggaaatccc
    56581 ggcaaagccg gtgatcgcgc ccagccattc ctccaattgt tggaacagca cttgccagcc
    56641 tcgggtttgg cgcaacggtg caaatgggtg caggcgcgtc agctcggacc aggtaagcgg
    56701 caacatctcg acggcggcat tcagcttcat ggtgcaggag ccgagcggaa tcatggaagt
    56761 cgtcagtgaa aggtcgcgcg cttccagtcg cttgaggtag cgcatcattt cggtttccga
    56821 gtgatagcgg ttgaagaccg gatgggtcag ataggaagac gtgcgcgcaa aagggacagg
    56881 ataggcaact tcggcttccg cgaccaggtt cgtcagttcg aaagcggggg tctggccgtc
    56941 atgaaaaacc cggataatcc cagccaggtc ctgctcggtg gttgtctcat ccagcgagat
    57001 gccgagggta tgctcatcaa ttaaccggaa attcatgccc tggctttcag cggctttgag
    57061 caggtcggct gccttctttt ggccgagcgc aatgcggaga gtgtcgaaga ataaacccga
    57121 gccgacctca tagccgagcc gctccaggct gcgcgccaaa acaaccgtgc gctgatggat
    57181 gcgctgtgca atttgccgta atccagccgg cccatgatag ctgacataca tggcggccat
    57241 acaggccggc agtgcctgcg cggtgcaaat attgctggtg gccttctcgc gccggatgtg
    57301 ctgctcgcgg gtttgcaggg cgaggcgcag ggcggggcgg cctcgtgcgt ccttcgagac
    57361 gccgacaata cgccccggca gaaggcgttt aaaggtgtcg cgcgtcgcaa aataagccgc
    57421 atgcggcccg ccatagccaa gtggcacgcc cagtcgctgc gtgctgccca gggctatatc
    57481 ggccccgaat tcgcccggcg gccggagcag ggttaggctc agcaggtcgg cggccacggc
    57541 gaccagcgct cccgcggcat gagcctgttc gataaaagtg gagtaatcta caacctcacc
    57601 ccaggtattc ggatactgca ccagagcacc aaagacctgg tcatggaatt gaaacgcgcg
    57661 gtgatctcca gtttcaaccc ggagagccag ggcgtcagcg cgggtcttta ccacctcaat
    57721 ggtttgagga tggcactctg cggagacaaa gaagacgctc cgcgccggct tgagcgagtg
    57781 gcacagggtc atggcttccg cagcggcggt ggcctcatcg agcagcgaag cattggccac
    57841 ttccaacccg gtgaggtcgg ttaccatcgt ttggaagttg agcaaagcct caaggcgtcc
    57901 ctgtgcgatt tcggcttggt aaggggtgta cggcgtgtac cagccggggt tctccaacag
    57961 gttgcgttga ataacggcgg gcacgtggca atcgtagtag cccatgccaa tatacgaacg
    58021 gaagatctgg ttctgcgcgg cgattttctt taaagccgcg agcacgtcag gctcgctgcg
    58081 ggcgggcggg agttgaagcg gttgccgcag acgtatggcc ggaggcactg cgtcgtcaat
    58141 cagtgccgcg agactcgtat agcccagcag gcggagcatt tcttcggcct cgctcgcgcg
    58201 cgggccaatg tggcggtgcc gaaaccgatc tgccggcgga ggcagatggg catttacagg
    58261 gcccgcggct acgctggccg gggacgaatt gttccgatca cagcttgcag gttcgataac
    58321 agacattcgg ggaacctacg gagagctgtc cgcaaatcaa gcgagatttg gtcccggctg
    58381 atttggcgtg tccgttttat cgttggcgct tctatttgga tgaggtaggc tgagacggta
    58441 gaatgaaatc acgaacaaaa acaacacaga agcaaacccg gcgcataacc aaacagctcg
    58501 ctattttcct ggataaccgg ccgggaatgc tggcgcgtgt ggcggatgca ctggcggatg
    58561 ccaaaatcaa tatttacgcc atcaccacca gtgatacggt ggaccacacg gtcattcgct
    58621 tagtggtgga cgattatcgg cgggcactac acgtctgcga agaacgcggc tctctggtcg
    58681 tcgaggatga tgtgctaatg gtggagggcg acaataagcc gggagaaatg gctcgcttgg
    58741 cccgtaagct ggcaaaggct gggattaaca tcgaatattg ctacagcgcc acgctgccgg
    58801 gcgccgggaa ggggttgctc atcttgcgcg tttccaaccc gaccaaagcg ctgaaagtgc
    58861 tgaaacttta agcaatcgca ggcgcatggt accgactcgt tcgggctgag ggagggcgtg
    58921 cagatgtccg atccccttct ttatgagatt aatacgcgct gttggctgca caggctttcc
    58981 gatcggtaca gcgcaccgat tacccttgcc aatattcccg cagatgaact ggcgcggtgg
    59041 cgaaagctag gctttacgca tatctggttg atgggggttt gggcggccgg tccactcgca
    59101 cgcgcgaaag ccttgcaata ttctgatttg cgccgggcct atgaccagtc cctgccaggc
    59161 tggcgcgaga cggatgttgc cggttcacct tacgcgattg gcgattacca ggtcccaccc
    59221 gcgctgggtg gcgaggcggg gctcgcggca tttcggcggc aacttcatgc gcagggcatg
    59281 aaattggtgc tggatttcgt gcccaaccat cttgggttgg accatgcctg ggtccgggag
    59341 cagccggagt tgtttgtgca aagcaccggg gaggcggctg gcacgttcgc gcagcagtct
    59401 ggcgggcaga ttcgctggct ggcctatggc aaggatcctt atttcccgcc ttggacggat
    59461 actgttcaac tggactatcg gcgcgatacg acacgcggtg cgatgacgga actgctcgcc
    59521 tccatcgccc ggcactgcga cggtgttcgc tgcgatatgg cgatgttggt gctcaacggg
    59581 gttttcgcca aaacctggaa acattttccc gtcacaactt ccccccccgt tgaggagttt
    59641 tggacgacgg ccattgccag gatccgccag gcgcatccgg acttcctgtt tctggccgag
    59701 gcttactggg gcttggagcc ggaattgcgc cggcttggtt ttgattatac ctatgacaag
    59761 acgctctatg accatctaat agcccatgat gccacagacg tgcagcggca tttactggga
    59821 ctggcagcgg ggcagcttgc tgccggtgcg cactttcttg aaaaccacga cgagccgcgt
    59881 gttgccgccc tgctgtcgct ggccgaacat cgggcggcgg cactgtgtat tctgggattg
    59941 cccggaatgc gatttctgca cgatggccag cttgaggggg cacgcattaa gatacccgtc
    60001 caattagcgc gcgtagcccc tgaagccggg cagtctgaaa tccaggccct ctatcagcaa
    60061 ctgctaggca cactacccgg cacggcggtt gggcggggcc gggccgtttt gtgccaaccg
    60121 cgagccgcct ggtcaggcaa tccgaccgct gccaatttaa tcatcgtgca atggcagcaa
    60181 cagacaccag agtttgattt ggtggtcgtg aacctggctc ctcatcgtag ccagtgctat
    60241 gtaccgttgt ccgttccgca actggccgcg cgttcctggg tcatgaaaga cctcctgggg
    60301 tcggagtgct accaacgtgc cggtgacgat cttcagaccc aaggcctcta ccttgatctc
    60361 ccgccccacg gcgctcagtt gttccatttt caaccagggg aattggtcaa gggcgcacgg
    60421 ttataaagcg caccgggctt gaatggcacc cgcccagagc aggccagttc gccagccaac
    60481 aaagtctgtc tttgcgccta cggggttttg ctttgcgact ggaacaggct gggggcgctg
    60541 gcctcgccat tgcgattaac tgcggaaacc gccactacct ccggagttgt gccgctccaa
    60601 gtatgtgagc tttggccgcc ggggaggatc tgcgttgacc aggtgccgcc cgagcgtgtt
    60661 tgcaccagcc agagccatgc cttgcctggc cccgccaggg cccaggaaag ggtgagcgat
    60721 cccgcgcgtt gcggggaggc gagggtaagt tgcggtttgg cgggggtagc ccgtcccagc
    60781 cagggcgaaa gcggcatcaa cgccggttgc tggtaggttt cacggacaag agcctcgccg
    60841 agagcgttgt tgcgcataag gctcttcata ttccaatgga tatggccgcc gacgccggcc
    60901 tgcttgcggg tcaggcgaat ttggctgaca atttcctggg gcgaccaacc gctgctggac
    60961 ggtgatggcc cgcttacttt ggtggaattc atcccggggc aaagcgttcg ccccttggga
    61021 ttctgttccg cccaccattg gagcaagaca gggaaacttt gcgccggcgg ttcgattgcc
    61081 caataaagtt gcggcgcgaa gtaatccgcc cagccattga tcagccactt gcgcgaatcg
    61141 gcgtaaaggg tcgcgtaggc atcgaggcct ttgatctggg ccggtgagtt ggggcgccag
    61201 atgccgaagg gactgatgcc aaacttgacc cagggctttg ttgttttaat ggatttatac
    61261 acctgctgga taaagttgtt gacgttttcg cggcgccagt cgtcacggct catctttccg
    61321 gcgacacccg aacgttgcca actcgcgttg tccgggaagt ccagttcctt tcccgcccgg
    61381 tctctcgctt tatagggata gaagtagtcg tcgaagtgga taccgtccac gtcgtagcgc
    61441 tttaccacat ccatcaccac gctcagcgaa tagtcctgca ccgctttttc gcccgggtcc
    61501 agccacagga actccccgta ttcccggaca agctgcgggc gagtcttgct gacatggttc
    61561 gccgcaaccg gcgacgcagc ggagcggagt cgcgcccgat aaggattgaa ccaggcgtgc
    61621 agttccaagc cacgcttatg cgcctcttcg atggcaaaag cgagtggatc gtagaacggt
    61681 tccggtgcct tgcccatggt gcccgtcagg tattccgacc acggctcgat tcgggaggca
    61741 tagagcgcgt cgcaggcggg gcggacctga aagatgatgg catttagctt gagatgcaca
    61801 gcccggtcca ggatcgccag gagctcggtt ttctgttcct gcgtgcttaa ggttttgcgg
    61861 gaaggccagt ctatgttagc tacggtggct acccaggctc cgcggaattc ccgcaaggga
    61921 ggcgggggag tatcgttggc agcagcatgg cttgtcggtt gcgatgcgga aagcaccccc
    61981 atgagcgtta ggacaaccca cccgcgacgt gtcccgtgtc tcgggaagtg agaatgccag
    62041 ctattcgcag accagccagg cggcaaagaa tgggatgccc tgcccgatat tttggcgggg
    62101 cgcaagagct tggcccacag caaggagaaa cgattgaaga tcagctcgtc tatcatggga
    62161 agccacgtta tcagccaaaa acgaaaaaag ccagcttcta aaaaaaaata gtctcaatgc
    62221 ttgacaagtt tggccaagta tgaataatat gcccgtaata aatgagattg cggtcgttgt
    62281 taggttgttt tgtcctgtta ctcgcactaa ccgtgcgagc gggcattatt tacagcccag
    62341 ccaataacgt taatctgacc gttaatgatg gtgaccccat cggccatacc ttaaccgcca
    62401 ctgttagcgg gtatttgccc accatcatag acgttcgcgt gacgctcaac attgagggag
    62461 tggacggcgg cgggtggaat ggcgacttgt attgctattt aacctacaat ggcacgctcg
    62521 tgccgctgct gaatcgggtc ggcgttcaaa ctggcgatgc ctttggttat ggaacgatag
    62581 gaatgaacgt cactctggcc ggcgatccgg gaggctacga cgatattcac tggacagagt
    62641 ttcccacttc aggcggcacg tatgcgccag acggacggga aattagcccc ggcatgcctc
    62701 cgtcgtctgc accggatttt gatgcggatg gcacgatcgg attctctgca tataacggaa
    62761 tggatccgaa cgggacatgg acgcttttta ttgccgacat ggcagggggc gatcagctta
    62821 aattagtcag ttggagcctg cagattaccg cggtgccaga accggtgaac gtggcattgg
    62881 gtgtgtttgg ttccgtattt ttggtggttg ccgtcgggcg gcaccagcgc gtgcggaagc
    62941 ttgtctccca agccttggga cgttaaagcg ggtaagcacg ctgcaaggtt tctcaagtta
    63001 aaggtggcag tggggattgt gtcctcgggt actaaaagac ttcctaaagg cgtgaaacgc
    63061 ccaaaagccc ttttgcagga agtccttttc tgcaccttag ccggagcgat ttagcaggga
    63121 gggactttca ggcggttacg atggtgccga tgccttcatc ggtgaatatt tccagcagga
    63181 cgctgtgggg tacgcggcca tccacgaagg agactttttc gacgccggcc cgaatggcgg
    63241 ccaccgcgct atccaccttg ggaatcattc ccttgtcaac gatgccgttt tgtttcagtg
    63301 tctcgacctc gctgattttg aggcgcggga tgacgctgtc agggcttttg gggttatgca
    63361 acaggccggg cacatcgctc atgaagacca ggcgccgggc gttgagggcg atggcggcct
    63421 gggcggcggc aacgtcggca ttgcagttat agactttgcc gtcttcgccg cgggcggtag
    63481 gactgatgac gggagtgatg ccctggcgga ggcaatccag cagtggcgcg gtgttgacgc
    63541 ggataacttc acccacgtag ccgaggtcga gaggctggcc gtccgcgcct ttcacctggg
    63601 ccttgcggca gtgaaagatg tccgggccgg cgaagctgcg ggccaggccg cccagagcgt
    63661 taatggcggc caccacctcg gggttgattt cccgggagag cacctggtcg gcaatttgcg
    63721 ccgtggcctc gctggtcacg cgctgcccct gaaggaaggt tgcctggagg ccggcttttt
    63781 ccatggcgcg cgtgatggcc ttgccgccac cgtggacgac aacgggattg atttcgaccg
    63841 cttccaggaa cacgatatcc cgcgcgacac ggtggcgcac ttccgcgtcg ggcgaatcca
    63901 tgaagctgcc gccgtatttg atgacgaaag tggcgccgct gaagcgctgg atgtagggca
    63961 gggcttccag cagcgtgtgg gctttggcaa caaggttttg catgtgaatg cgaggggctt
    64021 agtggatttg tcgcaggtag gcggcgtaga ggtgttcgag atctttcttt tccaagccgg
    64081 ttcggatcac taaccgcgcc cgggtccggg cggtttggcc gggacgcagg tcctggccga
    64141 agagggagag gtacattgag cgatgaccct cggtttcaaa gggcgtgagc accgcaaagc
    64201 aatcccgcgg gggcgccatg atgatggctt gcaggccggt cggggggctg ctgcggaggc
    64261 ccagcgggcg agccagacgc gggcggatag cccagtcaac cgggctgggc ggaaacttcc
    64321 agcgcccgtc ctggatgatg gacgcggctt tgttatcgcg cgggaaagcc tgccagacgc
    64381 cggcgccgcg gccggccggc gtaaacactt tttgcccatg aatgcgcgtc agcacgcagg
    64441 cgttggtgaa ggtttcggta aagtaggatg ccaggaagga ctcgaaccgc gggaggcggg
    64501 ttttagccag cacccgggtt tcaaggtcga gggtgtccgg cgtggtccag cgatagacgg
    64561 cgctgagttc aaagggccgt tccgcagtgg aaggccagcg gacttccacg cttccgtccg
    64621 cgcacagcgt tgcctcgctg ggccagtccc aagccgcaga gccgtggcgc cggttggtgc
    64681 tgaagacgcg gtaatggcca aacagcccca tgctatggtc caggcgcgtg ccggtcggca
    64741 ggtgcacgat ttcggatagc ccgatggatt tgcccccggc gcgtaactgg ccctgcaata
    64801 cgccagtgtt gaaggtgtat ccattagtgc cgtcgggaag gaaggcgagg cgcggccggg
    64861 ccgaaggctg ggggggggcc tgagcgaagg cataagccga cacgccgagg aagagaaccc
    64921 tggcaagatg ccgcatcatg ctagcgtggc aaaagggttt cattttcacc aaacatgcag
    64981 tgactgtgtc agggaggaaa gagaggctca agacagtttt gggagacatt gtgagaagag
    65041 cgaagtcaaa cgaacaggct gacggaccgc attttgacag agtcaataag gccctggtcg
    65101 gtcagtttta actccggaat gggggagaga ctcaggaagg agagggtcat aaacggggtc
    65161 gccaaccggc aacccatcgc gcgagcggcg gcattcaggt cggcgatgcg gtcaataact
    65221 tccgggagcg gacgatggga gaccaaaccg gcaattggca gggggagcgc cgcccgcact
    65281 tttccgtcgc tgacggccac ctggccaccc tgcattttaa ccagttcctc gatagccagg
    65341 gtgatgtcgg catccgtaac gcccactgcg atcacgttgt gggcgtcgtg ggccactgtc
    65401 gaagccaagg cgccacaccg gagtttgaat ccccgcacaa agcccacgcc gacattgccc
    65461 gtggcatggt gccgttcgac cacgaccagt ttgaggatat cgcgcatgac atcggacgcg
    65521 acgtatcctc cttcgatccg gggcgtttca atggcctgcc gggtgacgat ttgatgcggt
    65581 accatctcga tgacccggat tcgggacgaa cctttggcgc ggacagcgaa atcgtcgggg
    65641 gcctggcagc gcagattcat ggtgctgcgc ggcgggggag ccagcgtgcg cggcggggcc
    65701 aggtattcgc cttccgccgc gaccaggatg ccttttttat aggtttgtcg gacgaccagc
    65761 ttcttgagat cgtcgaagac aatgaaatcc gcccagtagc gcggggcgat ggcgccgaag
    65821 ttgcgcaagc ggtaatgccg cgcggtgctg atcgtggcga tttgcaaggc ggccatcggc
    65881 ggcacccctt ttttgatggc caggcgcacg cagtggtcaa tgtggccctc ggccacgagg
    65941 tctcccgcga gtttgtcatc cgtggccatg gagcaattca tggcattgtg agaattgatg
    66001 agtggcgcca gagtggccag gttgcgctcg gtgctgcctt cccgcagcag gacgtgcatt
    66061 cccagacgca gtttttcccg ggcctcggcc agtttggtgg attcgtgatc cgactgcacg
    66121 ccggccagga cataggcgtt gagcagtcgg ccgcgcagtc ccggggcgtg cccatcaata
    66181 ttcttgccct tgccggcttg aattttggcc agctcgcttt ccaggccgag atagacgccg
    66241 ggaaagttca ttagctcgcc cacgccggcg atgcgttcgt cgccaatcat caaacggagg
    66301 tcttcgggcg tcacccgtgc gccagacgtt tcgagggcgg tggccggcac gcaggaggga
    66361 agcatgacga agaagtccac cggcaggttg gcgctcgact ccaggacgta gcggatgccg
    66421 tccagtccca ggacgttggc atattcgtgc gggtcaacca ccacggcgcc ggtgccatgc
    66481 ggcaccaccg cgcgcgcaaa ctcccgcggg gtgagcatcg agctttccac gtggaagtgg
    66541 ccgtcaatga aactcggtgc caggtaggcg cccttcaggt caatgatttg tctggcccga
    66601 tagtcgccca ggccgacgac ccgcccgtcg tccacggcta cgttggcacg gtagatttcg
    66661 ccgctgagaa cattgatgag gcgggcattt ttgaaaagcc gttcggcggg ccgttccccg
    66721 cgcgcaatgc tcagctttcg ctgaagttct cgcatagcca agcatgttcc ggggttttta
    66781 tctgcgcgag caaaaaagcg caaggttgca gcgcggagcc caccggggga aatcgaactg
    66841 tgcttctatt gcagggtgac gcggcgaatt aacctccgat cttagccgaa acaatttaga
    66901 tttaactcca attcgatctc acagaacgcg caagtttctg gttttacagg cgaaaaacat
    66961 tcggaaacca gtggccagac cggtgaattc gtgagccaaa cggctcgttg tggctatccg
    67021 ctggcctgtc aggcctgctt cggtcctggt ggaacgaagg gataaggttg aactacgcaa
    67081 cgccaacgtt tcagataacg tattcgcgaa gccaaaagcc cttcgaacac tcgcttgccc
    67141 ttgaaaagcc gctttttgcc agccctgact tcataaccgg ctgcttttac agaacttagt
    67201 aattacctta ccgtcctctc gcgtcgtttt ggcctttcca agcatccgga ttcattatcc
    67261 atatcccttg gatttaggct attcctacgc cgtgtacaag gggtgtacaa gggatgctca
    67321 ccgggagtcc atgggatttt a
//
LOCUS       DAOPQG010000002        66955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_532_length_66955_cov_14.798266, whole genome shotgun sequence.
ACCESSION   DAOPQG010000002 DAOPQG010000000
VERSION     DAOPQG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 66955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66955
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_532_length_66955_cov_14.798266"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(683..1123)
                     /locus_tag="PKI07_00260"
     CDS             complement(683..1123)
                     /locus_tag="PKI07_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49512.1"
                     /translation="MSTTNRTGNDEAPPNGENGRRIRLAFARPPRAVRAAARWAGASF
                     LHARVTPESSARQASAARAGLLLLALGLAAIPSAALNAPGFAAVTLSACGFGLLLASI
                     ISKDILPARARCAACGIFEPGRRQMAARAWNGGRRREANNCPKR"
     gene            complement(1269..1925)
                     /locus_tag="PKI07_00265"
     CDS             complement(1269..1925)
                     /locus_tag="PKI07_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 family protein"
                     /protein_id="HOC49513.1"
                     /translation="MKTSSARWPAIALALLCLAAAAGCRSTYYAAYEKFGVHKRDLLK
                     KRVTAARDGQQAAQEQFRDALTRLKEITAFSGGELERAHQALKTEYQRCVQRAQTVRQ
                     RIRDVETVAGDLFAEWEKEIQQIHTASLQAASRQQLDATRRRYEEMHQALANAEQSMA
                     PVLTQFNDYVLFLKHNLNAQAVASLKGEAAGIQDEITRLLESMNRSITRADEFIRHMN
                     "
     gene            2078..3157
                     /locus_tag="PKI07_00270"
     CDS             2078..3157
                     /locus_tag="PKI07_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOC49514.1"
                     /translation="MGLLDYLKTQLLEIIQWQDDSRDTISWRFPDEDKEIKRGAQLIV
                     RESQVAQFVYLGQFGDTFGPGKHTLVTDNIPILSTLKGWKYGLESPFKADVYFVNTRL
                     FTGNKWGTANPIMMRDADFGIVRVRAYGTFDFRVVEPRLFLKEVAGTDDHFRLDEFAD
                     TMRSRVVSVFSEAVASARVPVLDVAARYSELGAALLPAINPAMTGKYGLEIPSFIIEN
                     VSVPPEVEQAIDKRSSMAAIGNLNDYVKFQMAEAMTRGGGEAGGMAGAGAGLGAGLAM
                     GQQMAAALAAAPAAAPAAPAGAPPPAAAAGGLPDLLGPAEVARALGVSEADVLETLEK
                     GELKGKKIGSTWRVSRAALDEFLKS"
     gene            3245..4348
                     /locus_tag="PKI07_00275"
     CDS             3245..4348
                     /locus_tag="PKI07_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC49515.1"
                     /translation="MVEATAQQKFSCPACGAEAQWNPARQALICAFCGTTSPAQVKLD
                     ATGGQTIQEHDLVRALRAIPDDARGWQARKTSVRCQSCQAISVFDPERVGQRCDFCGS
                     SALVPYEEIKAAFRPESLLPMQVSEAQIRETIREWYRSRWFAPNRLRRAALTDTVKGL
                     YIPYWTFDAQAHADWTAESGYYYFETETYRDVAGKLQTRQVRKVRWQPSAGEVDQFFD
                     DELVAASRGVQPELLRKVEPFPTEQLRAYEAGYLAGWVVERYQLDLVGAAQQARAAMD
                     RQMHALCARAVPGDTHRNLNVRTSYSRQTFKHILVPVWLLTYHYGPRAFQVVINGFTG
                     AMAGQYPRSWVKITFAVLAGLLALGALVWLAGR"
     gene            complement(4371..5576)
                     /locus_tag="PKI07_00280"
     CDS             complement(4371..5576)
                     /locus_tag="PKI07_00280"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOC49516.1"
                     /translation="MKRFRLPRSDWKKWWPLRAGRRGWLALLLALPGLPLPAAEHAPD
                     AGNRVVLDIESTPENPRNSEGAFVTLQTGRILFCYTQFYGGGADESPARIVSVYSDDA
                     GRTWSRAPQVVVENTGHRNVMSVSLLRLQSGKIALFYLIKNSWHDCRPWMRLSTDEAR
                     TWSAPVRVVDAPGYFVLNNDRVIQLRQGRLIAPVAFHRARRSNPADSHSWDSRALDLW
                     YLSDDEGRTWREADDWWAAPLPSRTGLQEPGVVELADGRVLTWARTDLGAQWGCVSTN
                     AGRSWPPPAPTSLQSPASPASLKRVPGSADLLAVFNDHSGAFPFPPGRRTPLVAALSF
                     DAGKTWPARRLLEDDPQGMYCYTAIHFVEDAALLGYFDFRGLGDQGANRLRLRRVRLD
                     WLRAAAPGR"
     gene            complement(5670..8285)
                     /gene="clpB"
                     /locus_tag="PKI07_00285"
     CDS             complement(5670..8285)
                     /gene="clpB"
                     /locus_tag="PKI07_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOC49517.1"
                     /translation="MQFDRFTIKSQEALQNAQGIARQHAHQEVDGEHLLLALIGQTES
                     LIPDLLKKIGVALPQLQSELERELARRHKVQGASSLDVYLSPSLKQALDAAQAEAGKL
                     KDDYLSTEHLLLGLLDKGGPALKKIFQAHNLKREVVLRALADLRGNQRVTDANPEDKF
                     QALDKYGRDLTALARQGKIDPVIGRDDEIRRVMQVLTRRTKNNPVLIGEPGVGKTAIA
                     EGLARRIISGDVPETLKHKRLVAMDLGAMIAGAKYRGEFEDRLKAFLKEIVANEGKII
                     LFIDELHTLVGAGAAEGATDAANIMKPQLARGELRAIGATTLDEYRKHIEKDPALERR
                     FQPVLVSEPGVEATIAILRGLKERYEVHHGVRIQDGALVAAATLSHRYIADRFLPDKA
                     IDLIDEAASRLRMELDSLPTEIDQLERQIQQLEIEQNALRKEKDEASRERLQKLEQDL
                     ANFKEQSRQLKAQWQNEKAAINAVSIINSQIEQTKLELEQAQRRNDLNLAAQIQYGRL
                     PELQKKLAAAEKTLHEKPQGQRLLAQEVSEEDIAKVVAAWTGIPVARMLEGEREKLVR
                     MEERLEQRVVGQKEAVQAVANAVRRSRSGLQEPNRPIGSFIFLGPTGVGKTETARALA
                     EFLFDDENAMARIDMSEYMEKHAVARLIGAPPGYVGYEEGGQLSEAIRRRPYSVVLFD
                     EIEKAHHDVFNILLQVLDDGRLTDGQGRTVDFRNTLIIMTSNLGSPVIQEFYARAENS
                     ARSLQAEAELERLVKLELKAHFRPEFLNRVDDIIIFHNLDEQHLGRIVEIQLQRLEKR
                     LAQQQLSLVVDRSARQLLAREGFDPQFGARPLKRAIQDLVLNPLATRLLRGEFKPGDR
                     IKAVARDGAIEFEKQ"
     gene            8406..9632
                     /locus_tag="PKI07_00290"
     CDS             8406..9632
                     /locus_tag="PKI07_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOC49518.1"
                     /translation="MLIWLLVLVLLASLAGIGYRQGVVRVACALVGILMGALLAGPLG
                     KPLRPLLVALGLKTPPLAWLLAPVAAFLLVSILFKVAGYMVHQKVDVHFKYHAGDLRL
                     ALWERLSRRLGLCLGLCNGVIYIILIAVAIYPLSYWSFQMATEDQDPKWMRLVTRLGQ
                     DLQRTGFAKVAWAFNPMPPVWYDSADLAGLIYHNPLSQARLSRYPAFLSLAERPEFQE
                     MARDANFSELLLGRAPILKILEHSRAQAVLQNPELMRQIWDTVVPDLKDLPVFLDTRK
                     SPKYDPERILGRWIFNGNATMVLFQKGRPNITSKEMRAWKQWMLAVFAKTSFVAMTDK
                     QAILREVPPLKLPAPGVVPTGGAQTLNGEWENLNGKYQLSLSGGSRTEQLAATIEGDR
                     MTIASKEMGIVFDRED"
     gene            complement(9664..10179)
                     /locus_tag="PKI07_00295"
     CDS             complement(9664..10179)
                     /locus_tag="PKI07_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49519.1"
                     /translation="MSQDTQNFEPLRRLLALKRYEQPPPGYFQDFSQQVIIRIRSGER
                     GEESAFIERLLWEAPWLRRIWAALEARPILAGVSGVAVCALLLAGMIYSDKTEVTNFA
                     LIPVAETAADGAALATNTMGGHPFLTGAGTLAERPDANPLTAMPAEGAFLGDLTRLRA
                     QPASFNFSGGN"
     gene            complement(10182..10832)
                     /locus_tag="PKI07_00300"
     CDS             complement(10182..10832)
                     /locus_tag="PKI07_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC49520.1"
                     /translation="MAEPASRKAPPPAREPDGKPAALPELTPDQELALVTRARRGDLK
                     AYDELVRRHQERIYATIYHMTANHEDANDLAQETFIKAFQVLGSFRGGSSFYTWIYRI
                     AVNKTLNFLKQRKHQSQMSLDDLDFNAENDPDLVALISEKTPRREAGLAELQEKLNAA
                     LQKLSEPHRLVVTLHDVQGLSHEEIAKIMDCHVGTVRSRLFYARQQLQALLADYLS"
     gene            complement(10835..11899)
                     /locus_tag="PKI07_00305"
     CDS             complement(10835..11899)
                     /locus_tag="PKI07_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOC49521.1"
                     /translation="MFAVDSKAVCAPTAAVFQAELPGDWKQIIRPVEPFLAAVGQRLT
                     QQVAAFDSHLSGYAHYALNGQGKHLRAALVALTGRALGKVTDDHVTVAAIIELVHLAT
                     LVHDDVIDEAEMRRGQPTLAAHWDNERAVLFGDCLFAHALKLAADFPTPEICRAVASA
                     TNTVCSGELLQIQHRRDFRLGRAGYFKVLEMKTAELFALACELSARLSGVGGEPQAAL
                     RRFGLALGTAYQVYDDCVDLFGSETPAGKDLGRDLAKGKLTLPVLLVWERANAADRKL
                     LQGLVEAWQAGAIDRLAALLARYQAFPASLEVFHQYLETARQALQVLPAATPRDSLLA
                     LADYLGQQAEGLRPAPARGL"
     gene            complement(12164..12322)
                     /locus_tag="PKI07_00310"
     CDS             complement(12164..12322)
                     /locus_tag="PKI07_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49522.1"
                     /translation="MINVKSYVHPVGIPCTPLVHGALGWGENAIGKLALAGDCGVGVW
                     DGCMTEVG"
     gene            12794..15334
                     /locus_tag="PKI07_00315"
     CDS             12794..15334
                     /locus_tag="PKI07_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC49523.1"
                     /translation="MATFFGQPIELPALNQVLVPDLWQQQAVAALRDGQDVVVQAPTG
                     AGKTLIFELWSNQGRNRGQAIYTVPTRALANDKLAEWRGQGWDVGIATGDLAENLGAP
                     VVVATLETQKNRLIRGEGPVLLVVDEYQMLSDLDRGLNYELAIALAPPQTQLLLLSGS
                     VGNPQDVVKWLRRLGRQALLVRSDQRPVPQEEVYANQLSYHVPSEIRGYWPRLVAKAL
                     AEDLGPILIFAPRRQAAEAMAGELARQLPNPNPLPLSAGQKLLVGDPLARLLKSRVTY
                     HHSGLSYGARAGVIEPLAKAGQLRVVVATMGLAAGINFSLRSVALAAESYRRDEAEQL
                     IQPDELLQMFGRAGRRGLDETGYVLISANELRLLDAHPCHLARSGAVDWAALLGLMTT
                     AAQQGRNPFREAVRAQERLFTIKPIVLGVEESLRHPEAPCGLQTDAERARHVRKRVRE
                     MRNSRGEWETYPAPVPVPVREVWVMRRTEGAPPEAAAPPPADASPPGPPVLTPALLTP
                     AALEKVGTGSLCVLAEDAQGRTYGRAVTIADRLGSGRVLLAKWIRRLTNWHGRQVAAE
                     VWEGKLVPLVKEKLAQQRTPVIALREQERRILALVSLAELTLRVPVDRHGVALWRPPE
                     REVLPADCARCALVAVCRQLPTRTGAALLWRRLGLVDATGVPTLRGRVVSAFTQGDGL
                     AIAAALEDETYALDELIYDLANLAAGFRFAGEEVNRWGGRLGQVCHARYGLQSIPGYL
                     ENGVPPNYGAGAEQIVAAVHRNPASKQGWITEVLGAGDIDRMIIEWRSLLRQITHAPD
                     LEWPRWRALQVLARSVLNETESPTLTDLPPLEPHQTRRVDHRLLLRRH"
     gene            complement(15404..17725)
                     /gene="pnp"
                     /locus_tag="PKI07_00320"
     CDS             complement(15404..17725)
                     /gene="pnp"
                     /locus_tag="PKI07_00320"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOC49524.1"
                     /translation="MSEKVIAPVGDKQLIIETGKLAKQADGAVSVQLGETIVIVAAVA
                     ATKPKPGQDFFPLTVDYREKAAAAGKFPGGYFKREGRPTEKEILTSRLIDRPIRPLFP
                     KGWYNEVQVQSIVLSADGDNDPDMLAVIGASTALMVSDIPWAGPLGAVRVGRVNGQFI
                     ANPTHSQMLESDLDLVYVGNETDLVMYEGSAREISEADFNAALKFGHEAIQPAIAVQK
                     ELAARVGKPKRQITLNIVPEEILQEAKTLAGDRIIAALLTPGKLAREAAVAALTEEVG
                     LKLVEKFGAERVTEFVLKDAFYYIQKEAVRGLILRQGRRLDGRDFDTVRPIVSEVGLL
                     PRAHGSALFQRGETQAITLCTLGTGEDAQEFDAYTGGATEKKFILHYNFPNFSVGETG
                     RISGPGRREIGHGALAERSLEPMVPIQTYPYSLRVTCEIMESNGSSSMASVCGGCLAL
                     MDAGVPITRPVAGISIGLCTEYDSQGKLANYKLLTDIIGWEDAFCDMDCKIAGTEKGI
                     TGFQLDLKLPGIPHALMAEAVEKARVARLHVLAEMARTLAAPRPEISKYAPRITTLKI
                     NPEKIGLLIGPGGKNIKKLVEESGCEINIEDDGTVNIYSVSADGMKIAVDAIQGMVAE
                     AEVGKLYRGKVVTIKEFGAFVEFLPGKDGLVHISELANFRVKQVEDIVKMGDEIWVKC
                     LGVDEKGRVRLSRRAAMAERDQQMGGAPADGAPAAADGAPAPDGDAGRFPDDDETHGG
                     PPEHSGGDRDGQHGGDHGSRRNRRGRRGRRDRR"
     gene            complement(17938..18195)
                     /gene="rpsO"
                     /locus_tag="PKI07_00325"
     CDS             complement(17938..18195)
                     /gene="rpsO"
                     /locus_tag="PKI07_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOC49525.1"
                     /translation="MEVKAKTIDGFKLHERDTGSADVQIALLTQRINHLTEHLKNNTK
                     DHSSRRGLLMMVGQRRRLLDYLRSIDVNRYQTVTRKLKLRK"
     gene            complement(18423..19037)
                     /locus_tag="PKI07_00330"
     CDS             complement(18423..19037)
                     /locus_tag="PKI07_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOC49526.1"
                     /translation="MSFSQLPINVFDVVVIAMLGLGIYKGRKYGMSEELLRLLKWLAI
                     VFGCAFIYEPAGRMLGQYTTLLGRLSCYVLAYVGCAVLIVLLFAGIQRLLGGKLVGSD
                     VFGRAEYYLGMGSGMVRLGCILLAALALLNARYFSPTEVRAMEQFQDEVYGSDFFPTL
                     HTVQASVFDKSLTGPWIKRNLSFLFIKPTEPENKPFRQREAFIP"
     gene            complement(19389..20432)
                     /locus_tag="PKI07_00335"
     CDS             complement(19389..20432)
                     /locus_tag="PKI07_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC49527.1"
                     /translation="MATKRLVEKDPKLKKDDLIVIGGGGGFIGGALARYFRNKGFTRI
                     RVADKKPLYDWYQRVPGVESLCLDLSDEENCRRLCEGAVEVYNLAADMGGMGFIERFR
                     VECLRSVLINTHMIESAYRAGARRYFFSSSACAYNVTLQQDPNVRALKESDAYPAMAE
                     RGYGWEKLISEMFCEEYWAERGMKTAIARFHNVYGPWGTWDGGREKAPAAICRKVIAA
                     VDSGKHTINIWGDGTPTRSFMYIDDCVKGIDMIMHCDKLIATPINLGTSELVTINELV
                     SKVEKIAGVKLKREYDLTAPRGVAGRNSDNTFIKKVLHWEPNTPLDKGLRETYNWIKT
                     QYLARKAGKRVVD"
     gene            20652..20725
                     /locus_tag="PKI07_00340"
     tRNA            20652..20725
                     /locus_tag="PKI07_00340"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20684..20686,aa:Gln,seq:ttg)
     gene            20922..22145
                     /locus_tag="PKI07_00345"
     CDS             20922..22145
                     /locus_tag="PKI07_00345"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOC49528.1"
                     /translation="MSTNAPVVSRKINLRRPDVMAAVQAQVLSHYRSSLVERIRGNGY
                     VLAAGDLTVRLAKEFGFCYGVERAIDLAYAARQVFPDQPLYILGEIIHNPEVNDQIRA
                     MGIKFLSGREKDADIADLKEDDIVIIPAFGVEVSVRQQLEAKGCRFVDTTCGDVMSVW
                     KRVRQYSQDNVTSIIHGKAWHEETKATSSQTRVSGGGHYLVVFTLEETDYVCNYILQG
                     GDKAAFLEKFKGACSEGFDPDVHLRTIGVANQTTMLRGETEEVQRRLRRAITQKYGEA
                     GMEPHFRFFDTICGATQDRQDALSKLLQEPLDLLLVIGGYNSSNTSHLAEMGEAKLPT
                     YFIKNAAKMESDRLIRHYDQHKHQEVETRDWLPSGRATIGITAGASCPNNLIEDVIRR
                     LFALRGISLPPDAGA"
     gene            complement(22167..22823)
                     /locus_tag="PKI07_00350"
     CDS             complement(22167..22823)
                     /locus_tag="PKI07_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49529.1"
                     /translation="MTGSPAAARRRGCFFHGCLVGAVGLLLVLGGLLVALHYTWKTML
                     RFTDSQPAELPAALLSRSEKNQLKRRCAAFEAALREQRPAPALTLSADEINALIADDP
                     RLQGFKGKVYVHLAGGQLQSQLSVPLEEAGWPQLKGRYLNGTATFRLSFQNGALAIRP
                     QTIRVKGRPLPEIYMRAIRKWNLAAEAARQPQAGALLQELADIRVRDSQLTLEPQRKP
                     "
     gene            complement(22820..23719)
                     /locus_tag="PKI07_00355"
     CDS             complement(22820..23719)
                     /locus_tag="PKI07_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOC49530.1"
                     /translation="MRLFEAMIDANHRAVAGDPSAGLRPADFASELPIVALTCLDVRL
                     NPLLPEVLGVPEDQFIWLRNAGNIITDPLGSTVRSLALACAVEGGQEIAIIGHTDCPM
                     GQTTALALLDQFRSLGVERHQLPPNLNEFFGLFGSERANVIKAADIVRQSPLIGPKIP
                     VHGLLVDIETGQVDWLVNGYQTLDSAAPRPSPALADPGQPLDALKPLANLEIGGLKFP
                     EEKIGETLVQAGDWLAAKVQEWEAKLTPPEAPAPPAAAAPVVSATYEAWRREKERLQR
                     AAPPKIPTPPPIPPKLRFHKKAR"
     gene            complement(23742..23969)
                     /locus_tag="PKI07_00360"
     CDS             complement(23742..23969)
                     /locus_tag="PKI07_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOC49531.1"
                     /translation="MDDLGSLLAGAGIVGLMVGLLGLACFVFWVWMLIHAIRNKGLRD
                     TEKIIWVLVIVLVQVLGALIYFFVGRPKARG"
     gene            complement(23982..25511)
                     /locus_tag="PKI07_00365"
     CDS             complement(23982..25511)
                     /locus_tag="PKI07_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOC49532.1"
                     /translation="MSNYSILLAEASLGGTVASVVVGLVFVIVIFAAIWASRYVKVGP
                     NEVLVVSGRRHRYVDPDGMVQTRGFRVRKGGGTFVIPVIEKVDRLSLELLTIDVKQEQ
                     EVYTSKGVPVRVDGVAQIKVKGDDISIATAAEQFLSKDTAAVKNIAMQTLEGHLRAIL
                     GTMTVEDIYQNRDAFAQKVQEVAAGDMANMGLGIVSFTIRDIKDSQGYLDALGKPRIA
                     QVKRDAIIAQAEADRDATIRSAQANQAGQEAKFLADTKIAEAQRDYQTNVAQYQAAVN
                     QKKAEADLAYDLQKYKTGQLVKAEEVQVNIVEKQKQIELQQQEILRKQRELEANVQKP
                     ADAERYKVETLANARKFQLETEAAGAASAAKATGFAAADVVKATGTAEADANKARGLA
                     EAAVIEAQGKATAEAMRVKAESFKQYNEAAVVEMIIRVLPEVAGKISEPLAKTEKMVI
                     INSGPAAGGGASKLTGDVTQIVAQLPPVIESLTGIKFEKLLEQVPGLKKGAEKGEDKP
                     K"
     gene            complement(25553..26113)
                     /locus_tag="PKI07_00370"
     CDS             complement(25553..26113)
                     /locus_tag="PKI07_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOC49533.1"
                     /translation="MDFLVYSVCFGAGLLFAVVSALLGHLFGGHDVDADVGTGGHAEA
                     GFQDAGVPGLSPFSPTTITAFITAFGGLGMIFARVEATRSPWVSAPLALLGALAIAAG
                     VVWLFGTIFHKVDASSELRVATLVGMSATVITPIPAGGVGEIAYVQAGSRYTAPARTE
                     SGVPVASGQAVKIVHIVGTQFYVAPL"
     gene            26553..26831
                     /locus_tag="PKI07_00375"
     CDS             26553..26831
                     /locus_tag="PKI07_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOC49534.1"
                     /translation="MSRRSKKPKMQTFRYVAPAATSVLLVGDFTEWQQNALVMEKGAD
                     GAWAISVKLPPGKHSYLFIVDGKWCEDPECTVRVPNPFGGHDMVREVG"
     gene            27224..28981
                     /locus_tag="PKI07_00380"
     CDS             27224..28981
                     /locus_tag="PKI07_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOC49535.1"
                     /translation="MKQLILIDYAIIGVYVLASFAIGNYYLRRAGSSLDEYFLSGRKT
                     SWWLVAFSMAATNYSIDYPLAICKLVAKYGIMGTWYVWSLAMAAVATTFFFSRLWRRA
                     RVVTDAELIKYRYSGNIGNALRIFKSIYFGVIINCFVLGWVFRALTKVMTVLTPWNQW
                     YVLTFAVAVTVIYTISGGLCGVIMTDFVQYVIIIIGAVALAVYGVVHVGGLAAMSAKI
                     EALYPGKHFLAFSPGFTGGQLMPWSAFLVYLFIQWWANKYSDGGGKHIQRMSAARNEV
                     HSVLGTFAYSLLYVLSTWPIIITALCALILFGKLPDPELGYPRLMSELLPNGVLGLCV
                     VGLLGAFMSTISTHFNLGPSYLINDIYKAFLVKNASEKHYILVSRCATGLVALTGMIL
                     SVFIESIADMWQFVLSFASGAGIIWILRWFWWRINAWSEFSSMICSAIVATYLKQAHP
                     EIPFESALLIIIAITVPVALLVTFLTPPVQDETLRDFYQRVRPGRWGWRRIAAKYGIP
                     RSPYLAKAVVNLLLGLALLFLVNFGVGTLLLRSRWLGIAELLAGALLALVLIKRIREE
                     EQLPDPQDSAPPSPGQGIV"
     gene            complement(29248..29481)
                     /locus_tag="PKI07_00385"
     CDS             complement(29248..29481)
                     /locus_tag="PKI07_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49536.1"
                     /translation="MKIKSNRKKRRPALANGQLWKTDKAYIQIVELGKRLLDYRMMRE
                     LGQARRIQTSSIETMEKYLQTHEAQLVKGNSRN"
     gene            29626..31152
                     /locus_tag="PKI07_00390"
     CDS             29626..31152
                     /locus_tag="PKI07_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOC49537.1"
                     /translation="MAFDSLRDFLGHLEAAGELRRLTQPVASELEITELADREMKKPG
                     GGQALLVEKPTVNGQISPFPLAINALGSYRRMALSLGAPSIEEAAAALGALVKAKPPA
                     SFKEALQLLGTAFDLRHARPKRVKDGACQEVIHHFDAPPPRTAPWPPAPDWRTPAAFD
                     PRPPTLLSLPIQQCWPLDGGRFITLPCVVTRDPDTGARNVGMYRMQVYDERTTGLHWQ
                     LQKVAARHGRRYYETGARMPVAVFLGGDPVYTFCATAPLPDGLDEFLLAGYLRQKSAE
                     LVRCQTCDLEVPADADFVLEGYVDPAEPLREEGPFGDHTGFYSLPDRYPVFHLTAITH
                     RQRAIYPATIVGPPPMEDYYIGSASVKLFLPILKMTFPELVDLALPAEGVFHNLVFVS
                     IKKTYPMQAFKIMHGLWGMGQMMFAKYIVVVDDDVDVHNSREVLFHLCANTDPQRDAL
                     FTKGPADVLDHATPVLGVGSKMGMDATRKLPGEGHPRDWPPLIQMPEAVRQKIRSMLA
                     "
     gene            31394..32647
                     /locus_tag="PKI07_00395"
     CDS             31394..32647
                     /locus_tag="PKI07_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49538.1"
                     /translation="MKPGARKPLRSREHPHMQPQIAGQPLPHRLNRRDFLRGAAAGMA
                     LAFLPPAAAAPAGPVRIETPFHGAILNPRQGRAVKRGLAIRVSGTAPAAMRVKVNGQT
                     ARREGDRFDAEVILRQPVTEIAAVAGASAAGGEDRIRVVWDRHSRPRYHFALDDNIYF
                     LRDIARQRYDSLFDCAYLKLLRNLNLKYGTRFSVNLYFAAEDGFTLAEFPDRYRGQWQ
                     DNAHWLKLAFHAYADKPDRPYQNAPAEKLLADYDQVAAQIHRFAGAATFAPPTNLHWS
                     MARPDALRALARRGVRCLSGYFRKDNGGWDINYGLDDRRSEYLSKHDLLMDFETGIIF
                     FRDAIVCNTVTPDQAAQVLAPLAAGPERRDPINLLCHEQYFWPFYPAYQPDYAQRIEA
                     AIRWTTEHGYQPSFFHEGILGSPEA"
     gene            complement(32692..35514)
                     /locus_tag="PKI07_00400"
     CDS             complement(32692..35514)
                     /locus_tag="PKI07_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC49539.1"
                     /translation="MLALLLAGPGWAAAAETESNAPPAGWSSQLTNIPAGFRVQAGFR
                     LELAAAEAPGAAPAAIAFDENGRLFVAETLADGDAGDNGGAVGRVRLLEDPDGTGQFR
                     SSRVYADKLAAVSAVACYGGGVFVAAGAAILYLKDAGSNGIATVRREVFSGFESTNAL
                     AARMAPHSFTWGLDNHIHAAGVGTDPALPSRCFSFAPRGLALAVGGGGAEAGLALDNW
                     GRALLCDPARPLRQALFPLRYRERNPFFPAPPEVADVLGPAATVFQLPPGDAAPPAAA
                     FLATNAPTAAWLTNARSCVVYRGHAFPTNFQGNVFIADASARVIHRVVLREAGLRLMA
                     ERAPDDARGEFLMSADPAFRPVQVANGPDGALYVVAAPADRAGGRIYRIVPQDFKPPK
                     AVRLGRATTAELVAALAHPNGWHRDTAARLLYERQDPAAAPALAKLFYYSRAPLTRAQ
                     ALYALEGLGALSAEPLLRALKDPDARVREQAVRLAEARARRGALPAPIWGQLKSLAAD
                     PSLRVRYQLALTLGEIRAPDRAPVLAELLWEAGDDPWRQAAVLSSLGEGAAEVLIALA
                     RQRAVRDSAAGQELLDRLAAMIGAQGQPEEIARVLAFADQTPPDSARALPTLAAMSEG
                     LRRAGISLARMNPQGPLPRFYAEALTVMPNVQFPEPVRLGAIRLLPSCPYTYLETADH
                     LLLLAGSRQSEALRSAALATLGSYDDPRLPDALLRRWRALTPALRAAAAAALLARADR
                     LGVVLDALEEGRLGLADLTDDQVTLLRAYPEPAIAERAARLLGPLRARRPEALQHFQP
                     ALNVRGAADRGRGVFLAYCAVCHQPGPAGPGLGPELATAPMRGKEWALAAMLEPSAEI
                     RPGNELYLARTAAGELFWGRLRDDHLAAVTLQQANGQPVVLPRLNLRHLAPLPGSLMP
                     EALAEGLSLPDMADLLEYLAGERRRP"
     gene            35923..36723
                     /locus_tag="PKI07_00405"
     CDS             35923..36723
                     /locus_tag="PKI07_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOC49540.1"
                     /translation="MEEPLLIPEPLPEAPPPPAMPLAARLLNVFAIPGAVFADVRACR
                     GSAANWIVPALLLGLAGMLTAMAVLSQPDFQQQMNKRFAEQAQALEQQVKAGKIKPAE
                     AERLRAFTRTFARPEVLRSLGGTMAFGFGVVRVFWWALVLWLLGRAFLKIHVNYLKAL
                     EVAGLALMISVLGSVVSLLLMVNLPRLFSTQDWSQAVSDFNPSHKNYLLLGTANIFAL
                     WLVCVLSVGLARLADVPFRRAMWFVFAAWLIQQLCLVTLQGVTGQFML"
     gene            37635..38642
                     /locus_tag="PKI07_00410"
     CDS             37635..38642
                     /locus_tag="PKI07_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49541.1"
                     /translation="MSIARMPRWSVRQEQAEVSRLAWAFVFSLALHLAVFGGYRTGQK
                     LHLWQNLHWPAWLQPPRALTELIRKKEKPPPARTQEPPLMFVNVSPAQATAEPPKTAR
                     YYSDKNAHAANPDADKITDVPRITGRQTQVPRTEDIPRPKFVPLQPAPAAQTPQEAQP
                     EVRPKPADTPGDLVLAKPSPTPRLTDGDAATAQPARPRTLREARARLAEPRAAGEMMK
                     QEGGVSRRHAIASLDTRATPFGAYDAALVAAISQRWYTLLDQREYASDRRGKVVLQFR
                     LHHNGRVTDMTVAENTVGEVLGLLCQKAVLDPAPFASWPSDMRRTLGDTRNIQFTFYY
                     N"
     gene            38649..39260
                     /locus_tag="PKI07_00415"
     CDS             38649..39260
                     /locus_tag="PKI07_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOC49542.1"
                     /translation="MESLVYILLFITSVVGLTFIVERGIVLRWRRVVPAEVEAVVQAC
                     QSRADVPNLRRVCQQHKSPTSRLLLIGAEHLDWPRAEITDAIQTRARNEVARLERGLV
                     VLEIIVGIAPLLGLVGTILGMMTAFGDVGQGGQIDPAELAKGISLILRATLFGLLIAI
                     PALVFWSYYTKKVESLALELESLCDEFLRRLYREEEPLPRGKS"
     gene            39292..39723
                     /locus_tag="PKI07_00420"
     CDS             39292..39723
                     /locus_tag="PKI07_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOC49543.1"
                     /translation="MALRFYTRKRREVPTVIIVALIDILIVLVIFLMVTTTFKQQPAL
                     KLALPESSQAQKTGASEAPPLVVSIDPQGQLRLGADARPVTLERLRQELLGAVAKTPE
                     LRLALSADKMAPFGQVVKVMDVAKEANLKVVNAYTKETGKP"
     gene            39692..40882
                     /locus_tag="PKI07_00425"
     CDS             39692..40882
                     /locus_tag="PKI07_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOC49544.1"
                     /translation="MPTPRKPASPDPAAARLRRFASGSAGARGKGRLWRFRRALSACG
                     AALGLAVSPAGGQPPPGLAAQKSGGDVVLSLTGAPGSGGTIQYRSGLSASNPWHYLAH
                     FAALSASPCQVTEPGALHAARFYRAFSYSLPTNVVPRSNMVWVAPGVFVMGSPAGEAL
                     RAANETQHTVTLTRGFYMNQYEVTQGEYLALMGTNPSRFTTQDYNGNPISPDLNRPVE
                     QVSWIDATNYCGQLTSQERTAGRLPAGWRYRLPTEAEWEYACRAGTATAFSFGNAIRG
                     GAANFQNHYEYDAGIGDLYVPGPEVPWRACTTAVGSYPPNAWGLYDLHGNVWEWCQDW
                     LGTYPAGSVTDPQGPASGSGRVYRGGSWDYWGRYSRSACRFSLAPSKQYDFIGFRVVL
                     APDP"
     gene            complement(40894..41997)
                     /locus_tag="PKI07_00430"
     CDS             complement(40894..41997)
                     /locus_tag="PKI07_00430"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC49545.1"
                     /translation="MNNEKIQFVDLVAQYQQLRTEVDAAMAKVCARGDFVLGEDVKLF
                     EQEFAAFCRAPHSVAVANGTEALKLALLACGIGAGDEVITCTHTFIATVSAIDQAGAR
                     AVLVDCDPRFYTIDPAQVERAITPRTKAIMPVHLYGQPADMDPLLEIARRHKLYVIED
                     ACQAHGAEYKRRRCGSMGDIAAFSFYPGKNLGAYGDGGAVTTTRADLAEQVRTLRNHG
                     QKVKYEHQIKGFNSRLDTLQAVVLRVKLRRLEQWNEARRQAAAKYDRLLAGTGLFTPP
                     VAPYAKPVFHLYVVQVPDRAKQQAAFDAANISHGIHYPIPVHLQPAFAELGYPPGSFP
                     VTEALVGKIISLPMFPELTDAQIERVAGACLKA"
     gene            42135..43154
                     /gene="gmd"
                     /locus_tag="PKI07_00435"
     CDS             42135..43154
                     /gene="gmd"
                     /locus_tag="PKI07_00435"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOC49546.1"
                     /translation="MAKKAFITGITGQDGSYLAELLLSKGYEVHGLIRRASTFNTARL
                     DPIYDDPHCDARRLFLHYGDLSDASALARLIGKIQPEEVYNLAAQSHVRVSFDSPEYT
                     ADITATGTVRLLEAIRETGIRPRFYQASSSEMFGLVQEVPQTERTPFYPRSPYGCAKV
                     FSYWATVNYRESYGLHASNGILFNHESPRRGETFVTRKITRAVAHILAGLQDKLYLGN
                     LEARRDWGYAREYVEAMWLMLQQDKPDDYVIATGETHSVDEFLTAAFAYAGLDREKHV
                     QIDARYYRPAEVDLLIGDASKARRQLGWAPKTRFPELVKLMVDADIELLRQRRQGQVR
                     SNRER"
     gene            complement(43318..43875)
                     /locus_tag="PKI07_00440"
     CDS             complement(43318..43875)
                     /locus_tag="PKI07_00440"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOC49547.1"
                     /translation="MSITRKTLGRIGMGNWVMVCLLFWSYPGRGEGLRWESVGVRGGI
                     PANQGCRNFNLAQVFGTWHLPLEWSLGKGWDVQGRLDLSGGWLGDRKYNAVIGSVGPS
                     LELHRQRCPLSLTAGVSPTYISRHDFELRDFGAAIQFTCHAGLNLDLARHWRLGYRWQ
                     HMSNAGLAAPNPGLNLHVFGVSFRF"
     gene            44148..45890
                     /locus_tag="PKI07_00445"
     CDS             44148..45890
                     /locus_tag="PKI07_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC49548.1"
                     /translation="MEISTIGRPRNVGWVRAGALLYGDWGASKAYVIGLGVAAVGFAA
                     LPHLLAVCLVTAIVGINYIWVCRCFANGGGVYTAAGLHSRRLAMAGGLLLLADFIVTA
                     SLSCLDAFHYLGFDQVEAKKWAISAIFLIGAMNFFGPKHAGSLAIWLALPVVAVVIVL
                     IGAGLPHLGQFHARAPSGGLLPNWVAFVGMILALSGVEAAASSTGVMKLDPGATVDKP
                     SVRIASRRAVTAVMLEVVIATAALSLLAMCLPVGEDQLAAHREDMLRFMGEIFVGPWF
                     GKVVGWVFALLLLSAVNTAIGGMVALLYVMAHDRELPHTFVQLNRFGVPWVALIAATI
                     LPVIVLDLNDSVEALSSLYAIGVVGAIVLDVGSCAFARTLPLTDRQRLVMKLTILLLA
                     AIWVTIALHKPRALVFVVMVLAVGFAIRQLARRHFLPPEVSAPAPVSGLRPVSGQFIG
                     QLILVAARGWTPALQFALEESRVRGSRLLVLYIREIAVKTGMSGNWQKDPAAKALFTR
                     LGAEAGDVQVSKLYSISDSPADTIIDIAATFGVDTVVLGGSRRAALVHLLKGNVVARV
                     AANLPESMHLIVIG"
     gene            45921..47354
                     /locus_tag="PKI07_00450"
     CDS             45921..47354
                     /locus_tag="PKI07_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC49549.1"
                     /translation="MATTRSSAGPAAGPAPPAREQTCRGPFAIMVLLFFLWGFMTVCN
                     DLLIPRFKSAFALSYFQAMLVPLAFFGAYFTGALLYFLASVLAGDPLARMGHQNGVVL
                     GLLISAAGSALFWPAASVPAYPLFLLALFIVGLGFAVLQIAANPYVALLGPERTAASR
                     LNLAEAFNSVGTTIGPLAGGYLIFDYFARSGAHGAESVKRPYLWFGLTLLLLAAVFWR
                     LRLPRVGGGRIERGVGALKYPHVVLGGVALFLYVGGEVSVGSLIINFLGQPGIAGLSE
                     LEASSYVSLYWGGLLIGRFMGAVALSALPPRRRQFWLAAFPLAGFLVLWGLRGGEAVW
                     GYLPFLALCWLLFQFSRARAGRTLLLFSAMAAALLFVALLAGGRLALWCVVGVGLFAS
                     IGWPNTFALALEGTGLYQGQASAWLVMGILGGGVLPPLLGGMADLTHSLQFALIVPLL
                     AFAGVALYGWKGHRVGRARPNSGRDCK"
     gene            47399..49930
                     /locus_tag="PKI07_00455"
     CDS             47399..49930
                     /locus_tag="PKI07_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOC49550.1"
                     /translation="MQNRPNHSEGAPNPGPPGRLPELLAPAGDWECARAAVENGADAI
                     YFGLEKFNARMRANNFTEADLPQLMEFLHRRGVRGYLTFNILVFEDELAAAEQYVRAM
                     ISAGVDAAVVQDVGMVRLIRQHSPDFPIHVSTQMTITSAAGLEFARALGANRVVLARE
                     CSLPEIAALRTPPAGLPPLPLEVFIHGALCVAYSGQCLTSEALGGRSANRGECAQACR
                     LPYELVADGQRVALGDRKYLLSPQDLAGLEVLPELVRLGVASLKIEGRLKSPEYVANI
                     TRIYRRALDQRGGDGRITRADRYGMEMAFSRGLHTGWFRGTHHQQLVHGRFGKKRGVF
                     LGPVARREGERVWVELQAPLKPGDGVVFDAGCPEQDEQGGRVYDVRRAGRGPENGRLA
                     ELRFGRGDLDFRRIHAGDKLWKTSDPELDRALRQSYAGDAPRFQRPLRMEARGAAGQP
                     LTLIVQDELGHEVRLASAMPLARAQSQPLTTPRLQAQLGRLGGTAFRLDALQNSLAGE
                     VVLPVSELNRLRREAVRQLDARRARPRQWTLRNDAPDAARANAAPPPAPPGAPPHLIV
                     LVRSQAQLDAALHAGVETVYCEWEDLKKYREAVAAARRHAAATGRRVEIFAAPPRIFK
                     AGEEWTLRQVRASEADGYLVRNYDHLRFFARDRCVGDYSLNIANRLAADYFQRQFGLE
                     RITASYDLNAAQLAALLRGAPPEWFEITIHQHMPMFHMEHCVFCAFLTSSADYRTCGR
                     PCDTREVKLRDRVGAEHPVKADAGCRNTVFNARAQTGAEYVARLQALGARHFRLEFLD
                     ESPAQASRAIALYRQLLRGEISAAQISRELKLLNQLGVTRSPPRL"
     gene            50062..51009
                     /locus_tag="PKI07_00460"
     CDS             50062..51009
                     /locus_tag="PKI07_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49551.1"
                     /translation="MKRTVCVFLACAAGAVGPLLGSDKPVPGSENVETVVSVDPATAE
                     EDMRREQAIAEFTARMKAANYPALFEAAAKEFNVPADILKAVAFAETRWEHLTWPPGE
                     TESPKNGMPRPYGIMSLWDNKYFGHSLLEAAALIGKTPEELKNDPYQNIRGGAALLRQ
                     HYDANPKPEGTTEADIESWRYAIRKYCGIPEPDLNARHALDVYVFMSQGYHQYGIEWD
                     GRPVNLDPIREETRRIVAEEEAKQAAAQADEAPAEAPPRPKLSSHPQPKPEAVMDEEI
                     TPPPPAQKSIKPWWIILAVVVVVAASFLLKRRLTRSDSA"
     gene            51115..52140
                     /locus_tag="PKI07_00465"
     CDS             51115..52140
                     /locus_tag="PKI07_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOC49552.1"
                     /translation="MKAVVLRGKEEMQVEHVPSPPLQAGEVRVQIEAALTCGTDLKVF
                     KRGYHAKMLIPPTRFGHEFSGAIAEVAPEVAGWRVGDRVAVANSAPCGQCFYCHQRQE
                     NLCDDLLFLNGAYARSIVVPARIVQKNLLRLKPETDFADAALVEPLACVVLGVEDAQL
                     RAGQRVLVIGAGPIGLMFVALAKHLGCQVSVAGRGKKRLETAQRLGADLLPSIEGPAG
                     LEAVLASGTRFDAVIEAVGKPETWAAAVRLVRKGGTVNFFGGCPAGTTLTLDTSLIHY
                     ANLTLRASFHHTPRTVRRALEFIEAGVVRADDFVDGECALSELPLLFRSMAAGNRAVK
                     TLVRVRE"
     gene            complement(52193..56221)
                     /locus_tag="PKI07_00470"
     CDS             complement(52193..56221)
                     /locus_tag="PKI07_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOC49553.1"
                     /translation="MPPLFRPIDWLSLAITFVVVWVVYLLTLAPELTLEDSGELVTGA
                     FYAGIPHPPGYPIWTIYSWLWTVLLPFGNMAWRVSVGQAFSGAMACGLLALLVSRGSS
                     MLMEGIEELKNMTGKWESAICLVAAVCAGLLLGLDGFMWNESVVVNRIAVTSVPWYLA
                     VLVCLLRWIYAPHQMRYAYWAAFLFGVCITLHQSLIVAALGIEVAIAAGNPRLGRDAF
                     LGNFIVYLVDCLLVVFTGDHMFHNIGAKPGLLFLFNAIGIGSLVAAIWLAVRTRGLGT
                     YWKTVLAMAGLWMLGVSFYLYMAVSGMSNPPMQWGYPRTVEGFFHALSRGQYEQPNPT
                     NPITDPTRFASQLGMLIEGVADEFTWVYMFIGLVPFLFFFKMQRRERAWIISLSALYL
                     CLGALLIMLLNPTPDRASADLVKVFLCSSHTIVACLIGYGLALTAAFMATHYQQFRLW
                     GLAGGMFAVVLALFCLWNTTGKHYFGPAGVIPLSELPHWVGRAFAPNQYGLPIYANLL
                     LLAIAVGFVLALLVYRQRGPLLVTLGLFAAMPVYSALTHWFPSNQRHHWFGYWFGHDM
                     FTPPFQDAAGQPLYPEMTRNAVLYGGTDPGRFCPTYIIFCESFTPHSCQPKEDQKFDR
                     RDVYIITQNALADGTYLCYIRAHYNRSTQIDPPFFRELFRMVLKDKDYQTNLLARAVA
                     PLDWAFTALGDRIEKRRRTYTSWLADRDFVDLPAFAARLRPGPQQDPVSKYLYENLSP
                     ATQHLLDAPGAEASLRRNLARDLNGLLERELKLKRRARDLQQEKAAVDQELADGSTSK
                     RLRRKQERLETELAQLEQARPFYEPERFRQVQLSEYLTDFIQENPQSWTRIRLNRLLL
                     EAAYPREIARSRGGVYPDREIYSATPEDSRRCFEEYVTDAQRRLQAGQLKPGEDVKLV
                     DNRVQVSGQVAVMNINGLLTKVIFDHNPKNEFFVEESFPLDWMYPHLTPFGIIMKINR
                     QPLPTLTEDILNRDHQFWRQFARRLTGDIVDYDTPVKQITDWIEKTYLRHDFTGFTGD
                     RKFVHDDDAQKSFSKLRSSIGGIYAWRLNPDPNICPPEYRPKSHEEYQRIFKEADFAF
                     RQAIAFCPYSPEAVFRYAQLLLQARRFDDARLVAETCLKLDPYNGQVKGLVDTIRGYQ
                     QSAGYEQAQITFQQLEEQVRQNPSNFQAALDLASAYLQLQQTNRTVQILEGILNHPQA
                     GADVFRSLIPVYSSLGHYADLQRAVDKLEKQPQLDAAAVLSLAQAYAVLQNVPKLEQS
                     LDALVKLMPTNPEAWYDLAALKCGLGKTAEALPALRQAVDLSQKRLQQDSKARDLLDT
                     ARKDERFRALRSSPEFQQVVPP"
     gene            complement(57035..57472)
                     /gene="nikR"
                     /locus_tag="PKI07_00475"
     CDS             complement(57035..57472)
                     /gene="nikR"
                     /locus_tag="PKI07_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOC49554.1"
                     /translation="MKPAKKPHVVRFTVSVPPDLATQLDRMTREKGYANRSLAVADLI
                     RAGLVEHRQNYGDREIAGSITLVYDHHKQHVQTTLTDIQHDHHHLILATLHVHLDHHN
                     CLEVLAVRGKAQTVKAIADELIAARGVKHGKLTITSTGKDLPP"
     gene            complement(57533..58213)
                     /locus_tag="PKI07_00480"
     CDS             complement(57533..58213)
                     /locus_tag="PKI07_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC49555.1"
                     /translation="MSRRRECEPARPAAPPRGAPGFTLIELLVVIAIIALLASLLLPA
                     IARAREKALVAKVHAELYGIGLALQMYADDHGGRVPPVRENCNNNLRDHWCQLPGELS
                     ESRYLPRSGEDGREANLEDPFSPGHTYKYAAPGPLLLNGEPAGNYALWVPTNFPNLKS
                     DHGKFYSQPAESPVRWVLWSLGPRPRSPRSQSSHAPMSQLTWYQRTGDDGVIVRFANR
                     DGMQFKSP"
     gene            complement(58231..59415)
                     /locus_tag="PKI07_00485"
     CDS             complement(58231..59415)
                     /locus_tag="PKI07_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49556.1"
                     /translation="MNVILATKPNETTSTPRTPARAGRWGALALAALLPAASALAETH
                     ANLQAVNAVGQSAWTPAYPLTVQGVLLTDPDEMLDATPNFQPAYVGTMGGEWQIVVQA
                     TAPGDRGGTFCWMGQNYATRRSPGDDSFSYSNSEWLAEIARLNHDPLTGHAFRKGDLV
                     SVTAQASLFYGGKRNINEMHRNEPDFDFTISLVSSNYGLPAPEVISLLSLMRPDDNNP
                     GTSEDIFDATRATGGEHWQSMRVRINGLTLLSTNGWNPTNTWGNRLCQVTDGANRMFR
                     LRHPRYDLGPAPDYVFDAIGILNQESNSGSQGTNGYELFVQQILPSEPPLLSVSGPAP
                     TVLSWPASLANYQLFSQPALAEGGPWLPVTNSPVLANDRWTVTLDPAAAPARFFRLQR
                     VR"
     gene            59426..59551
                     /locus_tag="PKI07_00490"
     CDS             59426..59551
                     /locus_tag="PKI07_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49557.1"
                     /translation="MKQGAAERKAKKPPAEGPAPAAGSAGRRRGRRRRGRAAGRE"
     gene            59602..60048
                     /locus_tag="PKI07_00495"
     CDS             59602..60048
                     /locus_tag="PKI07_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49558.1"
                     /translation="MAENIPNVPTGAMPPKKETGKVQPKKETVRINLPPKPSAAPTIK
                     LPTLPPGGPAGGPAAAAPVAAPGAAPAARPAGAPRAAAAPAAPAAPAAGAGRATPAAP
                     RMAAAPVAARPALGTLDTVLAIAAAVIGVLALLTTLNNMFEWIWKT"
     gene            60114..60446
                     /gene="trxA"
                     /locus_tag="PKI07_00500"
     CDS             60114..60446
                     /gene="trxA"
                     /locus_tag="PKI07_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC49559.1"
                     /translation="MAAPNIVNVTKATFAAEVLNAAGPVLVDFWAEWCGPCKAIGPIL
                     DELAAEYSGRVKIAKVNIDHEQELAAEYGVRAIPTLLLFQKGQVAEQMVGLRSKRELQ
                     NSLERVAA"
     gene            60515..61519
                     /locus_tag="PKI07_00505"
     CDS             60515..61519
                     /locus_tag="PKI07_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC49560.1"
                     /translation="MKAFIFTPGQLARRADFYYQLGQLTGAGLGLVQALEQVRRNPPA
                     NSYRAPLGRVLVQLAAGRTFSEALWQGGPWLPEFDLALLRAGEHSGRLEACFRLLADY
                     YTDRAQLARQMISNLVYPAFLLHFAVFIFPLATLLLSGDWVAYLGQTLGMLAPLYAGV
                     GLGIFAAQSQHGEWWRAVWEALLHPLPVLGPGRRCLALARLAAALEALLSAGVTIIEA
                     WELAAAASGSPALRRAVRAWQPQVKGGQTPAEAVRASGRFPELFATQYATGEISGRLD
                     DSLRRLRDYYQEAGARKLRAVTRWVPRAIYIGIMLLIAARVLLFYTRYFNLVRDAGGF
                     "
     gene            61663..62733
                     /locus_tag="PKI07_00510"
     CDS             61663..62733
                     /locus_tag="PKI07_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOC49561.1"
                     /translation="MRESSLAFLKTLVNTPSPTGYETRGQRVWLDYVAPFAEETFSDA
                     YGNCVAVLNRGGSPRLMLAAHADEIAMAVSYINDQGFIYVRKLGGVDAAITKAQRVII
                     HSRGGPVKGVVGNLAPHLMKEEKDPKPPRMHDLFIDIGVASRKEAAKLVRIGDPITLD
                     DEFELLRNDLAVARAFDNRVGTFAVAETLRLLRESPGRLRAEVCAVSNVQEEVGLLGA
                     RQIAYSLQPDLALVVDVTHATDYPAISLPQHGDIRVGRGPALTHGGCNHPAVVARLEA
                     VARAQRIPLQHEAMSASSGTDTDVIFWTRGGIASALVSLPNRYMHSPVEVVSLRDLEL
                     IPRLLAAFARSLAKGERFKVKI"
     gene            complement(62740..63429)
                     /locus_tag="PKI07_00515"
     CDS             complement(62740..63429)
                     /locus_tag="PKI07_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOC49562.1"
                     /translation="MHSLPPILVAGLTGIISGLLLSIPIGPINLTILNEGARRGFKWA
                     ALIGLGATTMEVIYCFIAFTGFATFFTRGYVKAAMELFSFVFLLFLGFKFLLSKSVHE
                     PPMRLRGAAERLEARLEERLHPHSAFMTGAVRVLGNVGVLVFWIILAANFISREWVTP
                     DWPGKLACVAGVAVATSAWFAGLSWAASLGHGKLSERTLLRMEHWSGAGLLILALIHG
                     GSIIWQMHRPH"
     gene            complement(63436..64317)
                     /gene="pssA"
                     /locus_tag="PKI07_00520"
     CDS             complement(63436..64317)
                     /gene="pssA"
                     /locus_tag="PKI07_00520"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOC49563.1"
                     /translation="MSAPPQKPAAPAPPRADADEPKLKIYFLPNLLTAGNLFCGFVAL
                     TKIVEANISGGDFQPVKVALGFILLACIFDLFDGRVARMGGVESPFGREFDSLADLIS
                     FGAAPAFLVHRVVLKDVFHGHPEWGWFIASIYLLCGAFRLARFNCLAAMKTAASREFL
                     GFPIPSAAGLVASLTLFMIWWDEKEFATGWWRYCLPVLLLFLSWMMVSNVKYPSFKTL
                     DLRATRTFTKTLVAILFLGSIMIFREKLLVFVLPVFFTAYLVYGFIRPHISRRMRREI
                     EDEEEDEEALPGPAAPG"
     gene            complement(64324..65001)
                     /locus_tag="PKI07_00525"
     CDS             complement(64324..65001)
                     /locus_tag="PKI07_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOC49564.1"
                     /translation="MKHSGKARKAGLKLIALSLPVLAAIGALGFLVPGAGRLIIPAVA
                     GVWLVFALFTLFFFRDPTPRVPAGAGLVLAAAHGTVDALEAGNEPEFMGGQCQRISIF
                     LSVLDVHVQNAPIAGKVAWLKYTKGRFLNAMRADSARHNENVLLGFTADEPPGRRVAV
                     RVIAGVIARRIVPFARLGDTVAPGERISLVQFGSRADVYLPADAKIRVKLGDHVVGGE
                     TVLAAWE"
     gene            complement(65041..66255)
                     /gene="dinB"
                     /locus_tag="PKI07_00530"
     CDS             complement(65041..66255)
                     /gene="dinB"
                     /locus_tag="PKI07_00530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOC49565.1"
                     /translation="MARPRAIVHLDADAFFASVEQAADPRLRGKPIAVGGERRGIIAS
                     ASYEARRLGIYTPMPTLLARRLCPRLILLPGDFDKYELFSRLMFSYAYDFTPDVEIGS
                     IDEGYFDVTGARQPALSIAEAIRRAIRQALKLSVSEGIGANKLVSQIASKINKPAAFL
                     GVPAGEEAAFLAPLSNQWLPGVGPQTANRLNAAGLVRIGQIAQTPPRLLEMVVGRLAP
                     QLREFARGVDDRPVVPARAPAKSYSEQETFAADTADEEYLEATLRRMGDRLMARVRAD
                     GKSIRTLTVKVRYNDMSEEQAGASLREPTDLETEIYGEIGRLLRQAWKRRVRLRLVAL
                     KLSNLYDGRFRAQLALDASVRQHEARQRLADAVDALRGKYGPGAVLRGHDFILRGKSG
                     APDRPPVPGRQL"
     gene            complement(66268..66897)
                     /gene="lexA"
                     /locus_tag="PKI07_00535"
     CDS             complement(66268..66897)
                     /gene="lexA"
                     /locus_tag="PKI07_00535"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOC49566.1"
                     /translation="MNELTERQQQVLGFIQENQDSAGLTPTLREIAAHFRFNSPNAAL
                     AHVQALMAKGFLRNRPGRARSLQVVAPNAPKNRPRPRVVSVPIYGSIPAGRPEDAAQE
                     EEGCVLIDVTTLGIQPTARTFGLKVRGDSMIGKSIVDGDIAIIEHGAPPRPGDVVAAL
                     IDGQVTLKTFLRQRGKPYLRAENPRYPNLIPQEELLIQGVMVALIRQRQ"
BASE COUNT        11018 a        22455 c        21948 g        11534 t
ORIGIN      
        1 aaacttccat ggttattgta atccaaggcc gggagagcgg cccggcggcc cgcgcgcgcg
       61 ggtcagttaa ccgggcagcg gtttgtcccc agccaccgga aaaagggtat caggccggtg
      121 gcggcggtca acgaagatag caacccgcca gattcgagcg cggtgccggg gaagaagctg
      181 ccatggtggg agcaaccggg gcgcgcgcct tccgaagcgg cgcgggaatg gtcgctcagt
      241 ttctgtggaa tggctttcgc ccgggagcgg aaaatacctg gcaagcccgg gtcaaggagg
      301 cggaggtttt cgcgggttgt gatttcgcgg aggcgagtcg gcaggggtgg cggggccgcg
      361 gacgctctca aacgggagag gatggctgcc gcgggaaggc ggcggcccga taaaaaaaag
      421 gcggcggcag ggttgctgcc gccgctttaa tcagactagc gttttaacgt ttggccttgg
      481 ctttggcttt ggctttggcg gcgggcttgg ccttggcttt ggctttcgct gcgggcttgg
      541 ccttgcagca gttcttggtt ttcttgctag ctttagctgc cattctgttt tcctttctac
      601 tggttctatt gctgtgtgtt gtttgttgtt tctgtctggt tgctccgaag cagcccggga
      661 cgaatgaatg actggcttcg tctcagcgct tcggacaatt gtttgcttcc cggcggcgcc
      721 cgccgttcca cgcgcgcgcg gccatttgcc ggcggcccgg ttcaaagatt ccacacgcgg
      781 cgcaacgggc gcgggcgggg aggatgtctt tgctgatgat ggatgcaagg agcaggccaa
      841 acccgcaggc ggacagggtg acggcggcaa atccgggcgc gttcagcgcg gcggacggga
      901 tcgccgccag gcccaacgcc aggagcagca ggccggcccg ggcggcggaa gcctgccgcg
      961 cggatgactc cggtgtgact cgcgcatgga ggaagctcgc gccggcccac cgcgccgcgg
     1021 cacggacggc gcgcggcggc cgcgcgaacg ccagccttat ccgccgtccg ttttccccgt
     1081 tcgggggagc ttcatcgttg ccggttctgt ttgtcgttga cacaacttcg cctgcgattg
     1141 aatcgaagat tatcactcac cgccaaacga tgcaatacta aattactaac aattttaccc
     1201 gcgatcagcg cgcgcgattt gtgcgcggcg cgcgcggaag cggcgccggc gcgggccgcg
     1261 gcgcgcgctc aattcatgtg ccggatgaac tcgtcggcgc gcgtgatcga gcggttcatg
     1321 gattcgagga ggcgggtgat ttcgtcctgg atgccggcgg cttcgccttt gagcgacgcg
     1381 acggcctggg cgttgaggtt gtgcttgagg aagagcacgt aatcgttgaa ctgggtcagg
     1441 acgggcgcca tgctttgctc ggcgttcgcc agggcctggt gcatttcctc gtagcggcgg
     1501 cgcgtggcgt cgagctgctg gcggctggcc gcctgcagcg acgcggtgtg gatctgctgg
     1561 atttcctttt cccattccgc gaacagatcg cccgccacgg tttccacgtc gcggatgcgc
     1621 tggcgcaccg tctgggcgcg ctggacgcag cgctggtatt cggtcttgag cgcctggtgg
     1681 gcgcgctcca gttcgccgcc cgagaaggcg gtgatttcct tgagccgggt gagcgcgtcg
     1741 cggaattgtt cctgcgcggc ttgctggccg tcgcgggccg cggtgacgcg tttcttcagc
     1801 aggtcgcgtt tgtggacgcc gaatttttca taggcggcat agtaggtgga gcggcagccg
     1861 gcggcggcgg ccaggcacag gagggccagc gcgatcgccg gccaccgggc ggaactggtt
     1921 ttcataaggc ttagcgcggg gcgcggcgcc cggcggccgg gggcgggcgt ttataattac
     1981 gccgtgattt tatactttcg cggccggggg tcgcagccca tactggaacg caggctatgg
     2041 tcggccgccg ttaaactcaa gcatagaaag acagcgtatg ggtctgctcg attatttgaa
     2101 gacgcaactc ctggagatta tccagtggca ggatgattcg cgcgacacga tttcgtggcg
     2161 ctttccggat gaggacaagg aaatcaagcg cggcgcgcag ctcattgtgc gcgagtcgca
     2221 ggtggcgcag tttgtgtacc tggggcagtt tggcgacacg ttcggcccgg gcaaacatac
     2281 gctcgtcacg gacaacattc ccattctttc gacgctcaag ggctggaagt atggcttgga
     2341 atccccgttc aaggcggatg tctatttcgt caacacgcgc ctgttcacgg gcaacaagtg
     2401 gggcacggcc aatcccatca tgatgcgcga cgcggatttc ggcattgtgc gcgtgcgcgc
     2461 ctacgggacg tttgatttcc gggtggtcga gccccggttg ttcctgaagg aagtcgccgg
     2521 gacggacgat catttccggc tggatgagtt tgccgacacc atgcgctcgc gcgtggtcag
     2581 cgtgttcagc gaggccgtgg cctcggcgcg cgtgccggtg ctggatgtgg ccgcgcgcta
     2641 ttcggagctg ggggcggcgc tgctgccggc gatcaacccg gccatgacgg gcaaatacgg
     2701 gctggaaatc ccgagcttca tcatcgagaa cgtgtcggtg ccgccggagg tcgagcaggc
     2761 cattgacaag cgttcgagca tggcggcgat tggcaacctg aatgattatg tgaagttcca
     2821 gatggccgag gccatgaccc ggggcggagg cgaggccggc ggcatggcgg gcgccggcgc
     2881 gggtttgggc gcgggcctgg cgatgggaca acagatggcg gccgcgctgg ccgcagcccc
     2941 ggcggcggct ccggccgcgc ccgcgggcgc cccgcccccg gcggccgccg cgggcggctt
     3001 gccggacctg ctcgggccgg cggaggtcgc gcgcgcgctg ggcgtcagcg aggcggacgt
     3061 gctggagacg ctggaaaagg gcgagctgaa gggcaagaag atcggctcca cctggcgcgt
     3121 ctcccgggcc gcgctggatg agttcctcaa gagttgacgg ggcggcgcgg cgcccgggag
     3181 cggcgggagg ctccccggtt accggcgcgg ccccggggcg attttcgtaa ctggatgcgg
     3241 cgccatggtt gaagccaccg cacagcaaaa gttctcctgc ccggcctgcg gcgccgaggc
     3301 acaatggaat cccgcccggc aggcgctgat ctgcgccttt tgcggcacga cttcgcccgc
     3361 gcaggtcaag ctggacgcca ccggcgggca aaccatccag gagcatgacc tggtccgcgc
     3421 gttgcgggcg atccccgatg acgcgcgcgg gtggcaggcg cggaagacct cggtgcgctg
     3481 ccagagctgc caggccattt ccgttttcga tcccgagcgc gtggggcagc ggtgtgattt
     3541 ctgcggttct tccgcgctgg tgccgtatga ggagatcaaa gcggcgttcc ggcccgaaag
     3601 cctcttgccg atgcaggtca gcgaggccca aatccgggag acgattcgcg agtggtatcg
     3661 cagccgctgg ttcgcgccca accggctccg gcgcgccgcg ctgaccgaca ccgtcaaggg
     3721 gctgtatatt ccgtattgga cgttcgacgc ccaggcgcac gcggattgga cggcggaatc
     3781 gggctactat tattttgaga cggagactta ccgggacgtc gccggcaagc tccagacccg
     3841 gcaggtgcgg aaagtgcgct ggcagccgtc cgcgggggag gtggaccagt tcttcgacga
     3901 tgagctggtg gccgcttcgc gcggggtgca gccggagctg ctgcgcaaag tcgagccgtt
     3961 cccgacggaa cagttgcggg cttacgaggc ggggtatttg gccggctggg tggtggagcg
     4021 gtatcagctt gacctcgtgg gcgcggcgca gcaggcgcgc gcggcgatgg accgccagat
     4081 gcacgccctc tgcgcgcggg cagtgccggg cgacacgcat cggaatctga atgtgcgcac
     4141 gagctattcc cggcagacgt tcaaacacat cctggtcccg gtctggctgc tcacttacca
     4201 ctacggcccg cgggcgtttc aggtggtcat caatggcttc accggggcca tggccggcca
     4261 atacccccgg agctgggtga aaatcacgtt cgccgtgctg gcggggctgc tggccctggg
     4321 cgcgctggtg tggctggcgg ggcgctgaac cgccgcgcgg cccggcggcg tcaacggccc
     4381 ggcgccgccg cccgcagcca atccaggcgg acgcgccgca gccgcagccg gtttgcgccc
     4441 tggtcgccca ggccgcggaa atcaaaatag cccagcagcg ccgcgtcttc gacgaaatga
     4501 atggccgtgt agcagtacat gccctgggga tcatcctcca ggagccggcg agcgggccag
     4561 gttttgcccg catcgaagga aagcgcggcc acgaggggag tgcgccgccc cgggggaaac
     4621 ggaaaggcgc cggagtgatc gttgaagacc gccagcaggt cggcggagcc gggcacgcgc
     4681 ttgaggctgg cgggcgaggc gggcgactgg agcgaggtcg gcgcgggcgg cggccacgac
     4741 cgcccggcgt tggtcgagac gcagccccat tgcgcgccca ggtcggttcg cgcccaggtc
     4801 aggacgcggc cgtcggctag ctccaccact cccggctctt gcaagccggt gcggctggga
     4861 agcggcgcgg cccaccagtc atcggcctcg cgccaggtcc gcccctcgtc atccgagaga
     4921 taccacaggt ccagcgcgcg ggaatcccag gagtgggaat ccgccgggtt ggagcggcgc
     4981 gcgcgatgaa acgccaccgg cgcgatcagc cggccctggc ggagttggat gacccggtca
     5041 ttgttcagca caaagtagcc gggcgcatcc accacccgca cgggtgcgga ccaggtccgg
     5101 gcttcatcgg ttgaaagccg catccagggg cggcaatcgt gccaactgtt cttgatgaga
     5161 tagaacagcg caatctttcc gctctggagt cgcagcagcg acacgctcat cacgttccgg
     5221 tggccggtgt tctccaccac cacctgcggc gcgcggctcc acgtccggcc ggcgtcgtcg
     5281 gaataaacgg acacgatccg ggcggggctt tcatccgcgc cgccgccgta gaactgggtg
     5341 tagcaaaaca gaatgcggcc ggtctggagc gtcacaaagg cgccctcgga attgcgcggg
     5401 ttttccgggg tggactcgat gtccagcacc acgcggttcc cggcgtccgg cgcgtgctcg
     5461 gcggccggca gcggcagccc gggcagcgca agcaggagcg ccagccagcc gcggcggccg
     5521 gcccggagcg gccaccactt tttccaatcg gagcgcggga gtctgaatcg cttcatggcc
     5581 aaaggaatgg tcggctcacg atagccgccg cgcccgcccg gggaaaggcc gaaagcgggc
     5641 cgcgcgggcg gaggcggacg gcgggaacgc tattgcttct cgaactcgat cgccccgtcc
     5701 cgggcgacgg ccttgatgcg gtcgccgggc ttgaactcgc cgcgcagcag ccgggtggcc
     5761 agcgggttca acaccaggtc ctggatggcg cgtttgagcg ggcgggcgcc gaattgcggg
     5821 tcgaagcctt cccgggcaag caactgcctg gcggagcggt ccaccaccag gctgagctgc
     5881 tgttgcgcca ggcgcttttc caatcgctgc agttgaatct ccacgatgcg gcccaggtgc
     5941 tgttcgtcca ggttgtggaa gatgatgatg tcatccacgc ggttgaggaa ctcgggccgg
     6001 aagtgcgcct tgagctccag cttcaccagg cgctccagct ccgcctcggc ttgcaggctg
     6061 cgggcggagt tttccgcccg ggcgtagaac tcctggatga ccggcgagcc gagattgctg
     6121 gtcatgatga tcagggtgtt gcggaaatcc acggtgcggc cctggccatc ggtgaggcgg
     6181 ccgtcgtcca gcacttgcag gaggatgttg aagacatcgt ggtgggcctt ctcaatctcg
     6241 tcgaacagca ccacgctgta gggccggcgg cgaatggcct cgctgagctg cccgccctcc
     6301 tcgtagccga cgtagccggg cggcgcgccg atgaggcgcg cgacggcgtg cttctccatg
     6361 tattcgctca tgtcaatgcg cgccatggcg ttctcgtcgt caaacaggaa ctcggccagc
     6421 gcgcgcgcgg tctcggtttt gccgacgccg gtggggccga ggaagatgaa cgagccgatg
     6481 gggcggttgg gctcctgaag cccgctgcgc gaccggcgga cggcgttggc gacggcctgg
     6541 acggcctcct tttgccccac cacccgctgc tccagccgct cctccatccg gaccagtttc
     6601 tcgcgctcgc cctccagcat ccgcgcgacg ggaatgcccg tccaggcggc gacgaccttg
     6661 gcaatgtcct cctcggagac ctcctgcgcg agcaggcgct ggccctgggg tttttcgtgc
     6721 agcgtctttt ccgccgccgc cagtttcttc tgcagttcgg gcaggcgccc gtactggatt
     6781 tgggccgcga ggtttaaatc gttgcggcgc tgggcctgct ccagctccag cttcgtctgc
     6841 tcgatctggc tgttgataat gctgacggcg ttgatggccg ccttttcgtt ctgccattgg
     6901 gctttcaact gccgggactg ctctttgaaa ttggcgaggt cctgctccag cttctggagc
     6961 cgctcgcgcg aggcctcgtc cttttccttc cggagcgcat tctgctcgat ctccagttgc
     7021 tggatttggc gctccaactg gtcaatctcg gtcggcaggg aatccagctc catgcgcagc
     7081 cgcgaggccg cctcgtcaat caggtcaatc gccttgtccg gcaggaagcg gtcggcgatg
     7141 tagcgatgcg aaagcgtcgc cgccgccacc agcgccccgt cctggatgcg caccccatga
     7201 tgcacctcgt agcgctcctt gaggccgcgc aggatggcga tggtcgcctc gacgccgggc
     7261 tcggacacca gcaccggctg gaagcgccgc tccagcgccg ggtctttttc gatgtgcttg
     7321 cggtattcat ccagcgtggt cgcgccgatg gcccgcagtt cgccgcgggc cagttgcggt
     7381 ttcataatgt tcgccgcgtc ggtcgcgccc tccgccgcgc ccgcgccgac cagggtatgc
     7441 agctcgtcaa tgaacaggat aatcttcccc tcgttggcca cgatctcttt gagaaacgcc
     7501 ttcagccggt cctcgaactc gccccggtat ttggcgccgg cgatcatggc gcccaggtcc
     7561 atggccacca ggcgtttatg cttgagcgtc tccggcacgt cgccgctgat gatccgccgc
     7621 gccaggccct cggcgatggc ggttttgccg acccccggct cgccgatgag caccgggttg
     7681 ttcttggtgc gccgggtcag cacctgcatc acccggcgga tctcgtcgtc ccgcccgatg
     7741 accgggtcaa ttttgccttg gcgcgccagc gcggtcaggt cgcggccata tttatccagc
     7801 gcctggaact tgtcctccgg gttggcgtcg gtcacgcgct ggtttccgcg cagatcggcg
     7861 agcgcccgca agacaacctc gcgtttgagg ttgtgggcct ggaaaatctt tttcagcgcc
     7921 ggcccgccct tgtcgagcag ccccagcagc aggtgctcgg tgctgagata gtcgtctttg
     7981 agcttgcccg cctcggcctg cgccgcgtcc agcgcctgct tgaggctggg gctgagatac
     8041 acgtccagcg aactggcgcc ctggacttta tggcggcggg ccagttcgcg ctccagttcc
     8101 gactggagct ggggcagggc gacgccgatc tttttcaaca aatccgggat caggctttcc
     8161 gtctggccga tcagggccag cagcaggtgc tcgccatcca cctcctggtg cgcgtgctgg
     8221 cgcgcgatcc cttgcgcgtt ttgcagcgcc tcctgcgatt tgatcgtaaa gcggtcgaat
     8281 tgcatatccg ctaatgatac gggagaaccg gctccggctc aagtccgggc ggcggggcgg
     8341 gcggcggcgg cggcgcgtat tttgttcgtg cttttttccc ccggccccct ttaataacgg
     8401 ccagtatgtt gatctggcta ctggttttgg tgctgctggc gtcgctggcg ggaattggct
     8461 accggcaggg cgtggtgcgc gtggcatgtg ctttggtggg cattttgatg ggcgccttgc
     8521 tggccggccc cctggggaaa ccgctccggc cgctgctggt ggcgctgggt ttgaagacgc
     8581 cgccgctggc atggctgctg gcaccggtgg ccgccttttt gctggtctcc atcctcttca
     8641 aggtcgccgg ctatatggtg caccagaagg tggacgttca tttcaaatac catgccggcg
     8701 acctgcggct ggcgctctgg gaacgcctga gccggcggct gggcctgtgc ctgggactct
     8761 gcaatggggt catttacata atcctgattg cggtggcgat ttacccgttg agctactgga
     8821 gcttccagat ggcgacggaa gaccaggacc ccaagtggat gcgcctcgtc acccggctgg
     8881 gccaggacct gcagcgcacc gggtttgcca aagtcgcgtg ggcgttcaac ccgatgccgc
     8941 ccgtctggta tgattccgcc gacctggccg gcttgatcta ccacaatccc ctgagccagg
     9001 cgcggctgtc ccgctacccg gctttcctca gcctggccga gcgcccggag ttccaggaga
     9061 tggcgcgcga cgcgaacttc agcgaactgc tgctggggcg cgcgccgatc ctgaagatcc
     9121 tcgaacattc ccgggcgcaa gccgtgctgc aaaaccccga gctgatgcgc cagatctggg
     9181 acaccgtggt gccggacctc aaggacctgc ccgttttcct ggacacccgc aaatccccca
     9241 agtacgaccc ggagcggatt ctcgggcgct ggatttttaa cgggaacgcc accatggtcc
     9301 tgttccagaa gggccggccc aatatcacgt ccaaggaaat gcgggcctgg aaacagtgga
     9361 tgctggccgt ctttgccaag accagctttg tggccatgac ggacaagcag gcgatcctcc
     9421 gggaagtgcc gcccttgaaa ctgcccgccc ccggggtggt cccgaccggc ggagcccaaa
     9481 ccctcaatgg cgagtgggag aacctgaacg gcaaatatca gctcagcctg tccggcggca
     9541 gccgcacgga acaactggcc gccaccatcg aaggcgaccg gatgacgatt gcgtcgaaag
     9601 agatgggaat cgtgttcgac cgggaagatt gatcccgcac tgagcccgcg ttaatcccgc
     9661 ggctcagttc ccgcccgaaa agttaaagct ggccggctgc gcccgcagcc gcgtcaggtc
     9721 tcccaaaaac gctccctcag ccggcatggc cgtcaacggg ttcgcatccg gcctttccgc
     9781 cagcgtcccg gccccggtca ggaacgggtg cccgcccatg gtattcgtcg ccagggccgc
     9841 tccatccgcc gccgtttccg ccacggggat caaagcaaaa ttcgtgacct ccgtcttatc
     9901 ggagtagatc atgcccgcca gcagcaaagc gcagaccgcc acaccggaga ccccggccag
     9961 gatgggccgg gcttccaggg ccgcccaaat gcgccgcagc cagggagcct cccacagcaa
    10021 ccgctcgatg aacgcggatt cctcgccgcg ctcgcccgac cggatgcgaa tgatcacctg
    10081 ctgggagaag tcctggaaat agcccggcgg cggctgctca tagcgcttga gggccagcaa
    10141 ccggcgcaac ggctcaaagt tttgggtgtc ctgactcata ctcaactcaa ataatccgcc
    10201 aacagcgctt gcaactgctg ccgggcgtaa aacaagcgag accgcaccgt gccgacgtgg
    10261 caatccataa ttttggcaat ctcttcatgc gataggccct gcacatcgtg cagcgtcact
    10321 accaatctat gtggttctga caacttctgc agggccgcgt tcaatttttc ctgcagctcc
    10381 gccagccccg cctcgcgccg cggcgtcttc tcggaaatca acgcgaccag gtcgggatcg
    10441 ttctccgcgt tgaaatccag atcatccagg ctcatctggg attgatgctt ccgctgcttg
    10501 agaaagttca gcgtcttgtt gaccgcgatg cggtagatcc aggtgtaaaa actggagccg
    10561 ccccggaacg agcccaggac ctgaaaggct ttgataaagg tttcctgcgc caggtcgttg
    10621 gcgtcctcat gattggcggt catgtggtag atggtggcgt aaatgcgctc ctgatgccgg
    10681 cgcaccaact catcatacgc cttcaagtcg ccccgtcgcg cccgcgtcac cagcgccagc
    10741 tcctggtccg gggtgagttc cggcagcgcg gccggcttgc cgtccggctc gcgggccggc
    10801 gggggcgctt tgcggctggc cggttccgcc ataatcatag accgcgcgcc ggcgccggtc
    10861 gcaacccctc ggcctgctgc cccaggtaat ccgcaagcgc cagcaagctg tcacgcgggg
    10921 tggcagccgg caaaacctgc aacgcctggc gcgcggtttc cagatactga tggaaaactt
    10981 cgagggacgc cgggaaggcc tggtagcggg cgagcagcgc cgccaggcga tcaatcgcgc
    11041 ccgcctgcca ggcttcgacc aggccctgca acagcttgcg gtcggccgcg ttcgcgcgct
    11101 cccagaccag cagcaccggc agcgtcagct tgcccttggc cagatcccgg cccaaatcct
    11161 tgccggccgg cgtttccgat ccgaacaaat ccacgcaatc atcatacacc tggtaggccg
    11221 tgcccagggc gaggccaaac cggcgcaagg ccgcctgcgg ctccccgccg acgccgctca
    11281 gccgggcgct cagctcgcaa gccagcgcga acagctcggc cgtcttcatc tccagcacct
    11341 tgaaataccc cgcgcggccc aggcggaaat cccgccggtg ctggatttgc aggagttccc
    11401 ccgagcacac cgtattggtg gccgacgcca ccgcgcggca aatctcgggc gtgggaaaat
    11461 ccgcggccag cttcaaggcg tgcgcaaaca ggcaatcccc gaacaggacg gcgcgctcgt
    11521 tatcccagtg cgcggccagc gtcggctgcc cgcggcgcat ctccgcctca tcaatcacat
    11581 cgtcatgcac cagggtggcc aggtgaacca attcaataat cgccgccacc gtcacatggt
    11641 cgtccgtgac cttgcccagc gcccggcccg tcagggccac cagcgccgcc cgcaaatgtt
    11701 tgccctggcc attgagcgcg tagtgggcat agccggagag atgcgaatca aacgcggcaa
    11761 cctgctgggt cagccgttgc cccaccgcgg ccagaaacgg ctccacggga cggataatct
    11821 gcttccaatc accgggcaat tccgcctgga acacggcagc agttggggca cagaccgctt
    11881 tggaatcaac ggcaaacatg tgtgcaaaat ctaagattgc caaccagaca agtcaaaccc
    11941 cttatccggc gcaatcaagc ctcccgcaaa agcaaatccc cgcccgcgat cctcccccgc
    12001 cacccccacg ctcctgcccg accttcggcg ccgcccaggc gcgcccacac ggaacccgcc
    12061 ccgccgcaaa accttccccg cgcctgcggc ccgcgccccg cccggtttcg cccgcgccat
    12121 gaccacaccc ccgctcgccc cgaacccgcc aatcggcaaa ccattaccca acctccgtca
    12181 tgcagccatc ccagacccca accccgcaat cccccgccag cgccagcttc ccaatggcgt
    12241 tctcccccca tcccaaagcg ccgtgtacaa ggggtgtaca agggatgccc accggatgca
    12301 cataggattt aacgttgatc atccggtggg catcccttgt acaccccttg tacacggctt
    12361 gctggtagct acggataagc ctggaatggc ggcagttagc ttgaaaatga cgggaggatt
    12421 gatttgtggg atcgaaagtg gggaactgtc caataattgg acagttttat gggaggaggg
    12481 aggaagcgga taattgtatt atccccgttc caaatgcgcg gccctggctg gttttgattc
    12541 tgccggaggt gttgcctgcc cggcgcaaag gccgtccggg cttggacccc gccgggcggc
    12601 tgccggttcc gctttggccg ggcagggggg cggcctggct cctttccact ttccggcgct
    12661 tccaaggcca tgctcgctga ggtgttcctt cttccggttt ggggttgccg gcaagggtcg
    12721 tggccgtggg ggccgggggt ggcgtcggtg ttttacaggt ttccgaacgg gcagagtcag
    12781 gtatcttacg gatgtggcaa cgttctttgg acaaccgatc gaactgccgg cgctcaacca
    12841 ggtcctggtt ccggatcttt ggcagcaaca ggcggtcgcc gccctgcgtg acggccagga
    12901 tgtggttgtt caggcgccca ccggcgcggg caagacgctg atcttcgagt tgtggtccaa
    12961 ccagggcagg aaccgcgggc aagcgattta taccgtgccg acgcgcgcgc tggccaacga
    13021 caagctggcg gaatggcggg ggcagggttg ggatgtgggg atagccaccg gcgacctggc
    13081 ggagaacctg ggcgcgcccg tggtggtggc caccctcgag acgcagaaga atcgtttgat
    13141 ccggggggag gggccggtgc tgctggtggt ggatgagtac cagatgctga gcgatctgga
    13201 tcgggggctg aactacgagc tggccattgc cctggcgccg ccgcagacgc agttgctgct
    13261 gctcagcggc agcgtgggca atccgcagga tgtggtcaag tggctgcgcc ggctggggcg
    13321 ccaggcgctc ctggtgcgct ccgatcagcg cccggtgccg caggaggagg tctatgccaa
    13381 ccaactgagc taccatgtgc ccagcgagat tcgcggctac tggccgcggc tggtggccaa
    13441 ggcgctggcg gaagacctgg ggcccatcct gatctttgcg ccgcggcggc aggccgccga
    13501 agccatggcg ggcgagctgg cgcggcaact gcccaacccc aacccgctcc cgctcagcgc
    13561 cgggcagaaa ctgctggtgg gcgaccccct ggcccggctg ctcaaaagcc gcgtcacgta
    13621 ccatcacagc ggcttgagct atggcgcgcg ggcgggggtc atcgagccgc tggccaaggc
    13681 cggccaactg cgggtggtgg tggccaccat gggcctggcc gccggcatta atttctcgct
    13741 gcgcagcgtc gcgctggccg ccgagtccta ccgccgcgac gaagcggagc agctcatcca
    13801 gccggatgag ttgctgcaaa tgttcggccg cgccgggcgg cggggcctgg acgagacggg
    13861 ctacgtcctg atcagcgcca atgaactgcg cctgctggac gcgcatccgt gccatctggc
    13921 gcgcagcggc gcggtggact gggcggcgct gctggggctg atgaccaccg ccgcccagca
    13981 gggccgcaac ccgttccgcg aagccgtccg cgcccaggaa cggctcttca ccatcaagcc
    14041 gattgtcctg ggcgtggagg aatccctgcg ccacccggag gcgccttgcg gcctgcagac
    14101 ggacgccgag cgggcgcgcc atgtccgcaa gcgcgtgcgc gagatgcgca acagccgggg
    14161 ggaatgggaa acttatccgg cgccggtgcc ggtgccggtg cgggaggtgt gggtgatgcg
    14221 gcggacggag ggcgcgccgc cggaggccgc cgcgccgccg ccggccgatg cctccccgcc
    14281 cgggccgccc gtcctgacgc cggccctgct cacgcccgcg gccctcgaaa aggttggcac
    14341 gggcagcctg tgcgttctcg cggaggacgc gcagggccgg acgtacggcc gcgccgtgac
    14401 catcgccgac cggctgggtt ccgggcgcgt cctgctggcc aagtggattc gccgtctgac
    14461 caactggcac gggcggcagg tggcggcgga ggtgtgggag gggaagctgg ttcccctggt
    14521 gaaagaaaag ctggcgcagc agcggacgcc ggtcatcgcg ctccgggagc aggagcggcg
    14581 catcctggcc ctggtcagcc tggccgagct gaccctgcgg gtgccggtgg atcggcatgg
    14641 cgtcgcgctt tggcggccgc cggagcgcga ggtgctcccc gccgactgcg cccgctgcgc
    14701 gctggtggcg gtgtgccgcc aactgcccac ccgcacgggc gccgcgctgc tctggcggcg
    14761 gctggggctg gtggatgcca cgggcgtgcc cacgctgcgc gggcgggtgg tgagcgcctt
    14821 cacccagggg gacggcctgg ccattgccgc ggcgctggaa gatgaaacct atgcactgga
    14881 cgagttgatc tatgacctgg ccaacctggc ggcggggttc cggttcgccg gcgaggaggt
    14941 gaatcgctgg ggcgggcggc tggggcaggt gtgccacgcg cggtatgggc tgcagagcat
    15001 tccgggctac ctcgagaacg gggtgccgcc caattacggc gcgggggcgg agcagatcgt
    15061 ggccgcggtg caccggaacc cggcgagcaa gcagggctgg attacggagg tgctgggggc
    15121 cggcgatatt gaccggatga ttatcgagtg gcgcagcctg ctgcggcaga ttacgcacgc
    15181 ccccgacctg gaatggccgc ggtggcgcgc gctgcaggtc ctggcccgga gcgtcctcaa
    15241 tgaaaccgag tcgcccacgc tcacggacct gccgccgctg gagccccacc agacccggcg
    15301 cgtggatcac cggctcctgc tgcggcggca ttaacgcggc cgccggaagc cccgggagac
    15361 cgccgccccg ccggggcggg acgcccgggc ggggggaaga ggattagcgc cggtcgcgcc
    15421 ggccgcgccg gccgcgccgg ttgcggcggg aaccgtggtc gccgccgtgc tgcccgtccc
    15481 gatcgccgcc ggagtgttcc ggcgggccgc cgtgggtttc gtcgtcatcg gggaaccggc
    15541 cggcgtcgcc gtccggagcg ggcgcgccgt ccgccgcggc gggcgcgccg tcggcgggag
    15601 cgccgcccat ctgttggtcg cgttcggcca tggcggcgcg gcgcgagagg cgcacgcggc
    15661 ctttttcgtc cacgccgagg cacttgaccc agatttcatc gcccattttg acgatgtctt
    15721 cgacctgttt gacgcggaag ttggccagtt cgctaatgtg gaccaggccg tccttgccgg
    15781 gcaggaactc gacgaaggcg ccgaactcct tgatggtgac caccttgccg cggtagagct
    15841 tgccgacctc ggcctcggcg accatgccct ggatggcgtc cacggcaatc ttcatcccgt
    15901 cggcggagac cgagtagatg ttgacggtgc cgtcgtcctc gatgttgatt tcgcagccgg
    15961 attcctcgac cagtttcttg atgttcttgc cgccggggcc gatgagcagg ccgatcttct
    16021 cggggttgat cttgagggtg gtgatgcgcg gggcgtattt gctgatctcg ggccgcgggg
    16081 cggccagggt ccgggccatc tcggccagca cgtgcaggcg cgccacgcgc gccttttcca
    16141 ccgcctcggc catcagggcg tgggggatgc cgggcagctt gagatccagt tggaagcccg
    16201 tgatgccctt ctcggtgccg gcgatcttgc aatccatgtc gcagaaggcg tcctcccagc
    16261 cgatgatgtc ggtcaggagc ttgtagtttg cgagcttgcc ctgggagtcg tactcggtgc
    16321 ataagccgat gctgatgccg gccacggggc gggtgatcgg cacgccggca tccatcaagg
    16381 ccaggcaccc gccgcagacg gaggccatgg acgaagagcc attggattcc atgatctcgc
    16441 aggtgacgcg cagggagtag ggataggtct ggatgggcac catgggctcg agcgaccgct
    16501 cggccagggc gccgtgcccg atctcgcgcc ggcccgggcc gctgatgcgg ccggtttcgc
    16561 cgacggagaa gttcgggaaa ttgtagtgca ggatgaactt cttttcggtg gcgccgccgg
    16621 tgtaggcgtc aaattcctgc gcgtcttccc cggtgcccag cgtgcagagc gtgatggcct
    16681 gggtttcgcc gcgctgaaac agcgccgagc cgtgcgcccg cggcaggagg cccacttcgc
    16741 tgacgatggg ccggacggtg tcgaagtcgc ggccatccag gcgccggccc tgccgcagga
    16801 tcaggccgcg gacggcttcc ttctggatgt aatagaaggc gtccttgagc acgaactcgg
    16861 tcacccgctc cgcgccaaac ttttcgacca gcttcaggcc cacttcctcc gtgagggccg
    16921 cgaccgcggc ttcgcgggcc agtttgccgg gcgtcagcag cgccgcgatg atgcggtcgc
    16981 cggccagggt tttggcttcc tggaggattt cctcgggcac gatattgagc gtgatttggc
    17041 gcttgggctt gccgacgcgg gcggccagct ctttctgcac ggcaatggcc ggctgaatgg
    17101 cctcgtggcc gaacttgagc gcggcgttaa agtccgcctc gctgatttcc cgggccgacc
    17161 cctcatacat gacgaggtcg gtttcgttgc cgacatagac caggtccagg tcgctttcga
    17221 gcatctggct gtgggtgggg ttggcgatga actggccgtt gacgcggccc acgcggaccg
    17281 cgcccagcgg gcccgcccag gggatgtcgc tgaccatgag ggcggtcgag gcgccgatga
    17341 ccgccagcat gtccgggtcg ttgtcgccgt cggcgctcag gacgatgctt tggacctgga
    17401 cttcgttgta ccagcctttg gggaagagcg ggcggatggg gcggtcaatg agccgcgagg
    17461 tgaggatttc cttttcggtg gggcggcctt cgcgtttgaa atagccgccc gggaacttgc
    17521 ccgccgccgc ggccttttcc cggtaatcca cggtcagcgg aaagaaatcc tggccgggtt
    17581 tgggtttggt ggcggccacg gccgcgacga tgacgatggt ttccccgagc tggacgctga
    17641 ccgcgccgtc ggcttgtttg gcgagtttcc ccgtttcgat gatgagctgt ttgtccccca
    17701 ccggggcaat gactttctct gacataatac tctacttccc gccgccgccg aggaacttca
    17761 gtgaatcccg aaaacgttcg ggactgaatg aaatcacccg gggcaacgga gctttctttt
    17821 acgcgagggt taagggtgcc agccgggctg tccgcgtttg ccagcctggt tgaatcaagg
    17881 tttccgcacc tgccggttca ttttgaatcc ttcgctcaca ggtcggcgga ggcgcaatta
    17941 tttgcgcagt ttaagtttcc gggtgacggt ctggtagcga ttcacgtcaa tggagcgcag
    18001 gtaatccaac aggcgccggc gctgcccgac catcatcagc aggccgcggc gggaactatg
    18061 gtctttggtg ttgtttttca gatgctcggt cagatggttg atgcgctgcg tgagcaacgc
    18121 aatttgcaca tccgccgagc cggtgtcccg ctcgtgcagc ttgaatccat ctatcgtttt
    18181 cgctttcact tccatacttt caagtccagc caatcagtcc ttcatttaat attcgaggcc
    18241 gagtctaggc ccccggcccc ggagtgtaaa gccctttcca caattcccgg gaaaaagcgc
    18301 ggaacggcgg cggggggcgc ggaccggccc cgttgccgcg gcgcgccccg ggaacggcgg
    18361 cgaggagcga ccggcggggg gcggcggccg ggccgggccg gagtgtgccg ggggcgcggg
    18421 gctcagggaa tgaaggcttc ccgctggcga aacggtttgt tctccggctc ggtcggcttg
    18481 atgaacagaa agctcagatt gcgtttgatc cacgggccgg tcagcgactt gtcaaaaacc
    18541 gaggcttgca ccgtgtggag cgtggggaag aaatcgctgc cgtacacttc atcctggaac
    18601 tgttccatgg cgcggacttc ggtcgggctg aagtagcggg cgttcaacag cgccagcgcc
    18661 gccagcagga tgcagcccag gcgcaccatg cccgagccca tgcccaggta atactccgcg
    18721 cgcccgaaca cgtcgctccc caccagcttg ccgcccagga ggcgctggat tccggcaaac
    18781 agcagcacga tcagcaccgc gcagccgaca tacgccagca cgtagcagga cagccgcccc
    18841 aggagggtgg tgtattgccc cagcatccgc cccgccggct cgtaaataaa cgcgcaccca
    18901 aagacgatgg ccagccactt gagcaggcgc agcagttcct cggacatgcc gtatttccgg
    18961 cctttgtaaa tgcccagccc cagcatggcg atgaccacca cgtcgaacac gttaatcggc
    19021 aactgactaa agctcataag tttgtcccgt catgcgcatc cccgggaggg atacggcgag
    19081 ggacttggta gccagtttgg tgccatgtgg aagcgccggg gagggaagcg aaacctccgc
    19141 cccgggggac gtgcggcgcg gccccacgcc cggctggcgg ccgggaaggc gcgcgcggcc
    19201 gcgcccggcc tggttgttcg ctggcgcggg cgcggcgggg cgccgccagc tttgcgccgg
    19261 gggcatccac tccccggcgc cgcagaccgg attccggagc cggcggcggc gcggccaggc
    19321 ggtctgtccc aggacgggac agttctgccc cgtctcggtc agggtggcgg ggcggggcgg
    19381 gggcgggctt aatccaccac ccgtttgccg gccttgcggg ccaggtactg cgttttgatc
    19441 cagttatagg tctcgcgcag ccctttatcc aacggcgtgt tgggctccca gtggagcacc
    19501 ttcttgatga acgtgttgtc gctgttgcgg cccgccactc cccgcggcgc ggtgaggtcg
    19561 tattcgcgct tcagcttgac gccggcgatt ttttcgacct tggacaccag ctcgttgatg
    19621 gtcaccagtt cgctcgtccc gaggttgatg ggggtggcga tgagcttgtc gcagtgcatg
    19681 atcatatcaa tgcccttcac gcaatcatca atatacatga agctgcgggt gggggtgcca
    19741 tcgccccaga tgttgatggt atgtttgccg ctatccaccg cggcgatgac cttgcggcag
    19801 atggccgccg gcgccttctc gcgtccgcca tcccaggtgc cccaggggcc atagacgttg
    19861 tggaaccgcg cgatggccgt cttcatcccg cgctcggccc aatactcctc gcagaacatc
    19921 tcggagatca gcttctccca gccgtagccg cgctcggcca tggccgggta ggcatcggac
    19981 tccttgaggg cgcgcacatt cgggtcctgc tgcagcgtga cattgtaggc gcaggcggag
    20041 gaggagaaga agtagcggcg cgcgcccgcg cggtacgcgg actcgatcat gtgggtgtta
    20101 atcagcacgc tgcgcaggca ttcgacgcga aaccgctcga tgaagcccat gccgcccatg
    20161 tccgccgcca ggttgtaaac ctcgaccgcg ccctcgcaca ggcgccggca attctcctcg
    20221 tcgctcagat ccaggcagag agattcgacc ccgggcacgc gctgatacca gtcgtagagc
    20281 ggtttcttgt ccgcgacccg gatgcgcgtg aagcccttgt tgcggaaata gcgcgccagc
    20341 gccccgccaa tgaacccgcc gccgccgcca atgacaatca aatcatcctt cttgagcttg
    20401 ggatcttttt caaccagtcg tttcgtagcc ataaactata ttctaatact ctgttcgttt
    20461 ctgttccggc ggctccgcgc gcgttgggcg cccaaggccg gctgccaccc gttgtttgcc
    20521 ggtttaagtg gcgttggaca cacactgcca ccccggccgc cggttgacga gttaaaaaga
    20581 gagcggggcc gggaaaggcg gtattttttc ctttgcatgg ggggcgccga ggcgctacgt
    20641 tatgcccgcc ttgtccggtg gtgtaatggt agcacaagag tctttggagc tctttgtcat
    20701 ggttcgaatc catgccggac agccatttcc tcagggaaaa caccatctcc cagcccggca
    20761 gcggcgccgc cgcggggccg ccaggccggc cggacgaggt tccacaccgg gccggggggc
    20821 gcgccgttct tcgccggggc gggccgccgc cggggggcgc gagcattccc gccgcggttt
    20881 ccactggacg gttgggcgac cggtgttaga gtgaaaatgg tatgtcaacc aatgcgcccg
    20941 ttgtctcccg gaagatcaac ttgcgccgcc cggatgtcat ggcggcagtt caggcccagg
    21001 tgctgtcgca ctatcgcagc agcctggtcg agcgcattcg cggcaacggg tatgtgctgg
    21061 cagcgggcga cctgacggtc cggctcgcca aggaattcgg cttttgctac ggcgtcgagc
    21121 gggccattga cctggcctat gccgcgcggc aggtcttccc cgatcagccg ctctacatcc
    21181 tcggcgaaat catccataac ccggaggtca acgatcaaat ccgcgcgatg ggcatcaagt
    21241 tcctctccgg ccgcgaaaag gacgcggaca tcgccgacct gaaggaggat gacattgtca
    21301 ttattcccgc tttcggggtg gaagtctccg tgcggcagca gctcgaagcc aaaggctgcc
    21361 ggtttgtgga tacgacctgc ggcgacgtca tgagcgtctg gaaacgcgtc cgccagtatt
    21421 cgcaggataa cgtcaccagc atcattcacg gcaaggcctg gcatgaggag accaaggcca
    21481 ccagctcgca aacgagggtc agcggcggcg gccattacct cgtcgtcttc acgctggagg
    21541 agacggacta cgtctgcaat tacatcctgc agggcggcga caaggccgcc ttcctcgaaa
    21601 agttcaaggg cgcctgctcg gaaggatttg accccgacgt tcacctccgg accatcggcg
    21661 tggccaacca aacgaccatg ctgcggggcg aaacggagga agtgcaacgc cgcctgcgcc
    21721 gcgccatcac gcagaagtac ggcgaggccg ggatggagcc gcatttccgc ttcttcgaca
    21781 ccatttgcgg cgccacccag gaccggcagg atgccctgag caagctcttg caggagccgc
    21841 tggacttgct cctggtcatc ggcggctata actcctccaa cacctcgcac ctggcggaga
    21901 tgggcgaagc gaagctgcct acttacttta tcaagaacgc cgccaagatg gagtctgacc
    21961 ggctgattcg ccattacgac cagcataaac atcaggaggt cgagacccgg gattggctgc
    22021 cttccgggcg cgccaccatc ggcatcacgg cgggggcctc ctgccccaac aacctcatcg
    22081 aggacgtgat ccgccggctg ttcgcgctgc ggggtatttc gctcccgccg gacgcgggcg
    22141 cgtaagcggc gcccgtttcc gggcggttac ggctttcgtt gcggctccag ggtaagctgg
    22201 ctgtcccgca cccggatgtc cgccagttct tgcaaaagcg ccccggcctg cggctggcgg
    22261 gcggcctccg ccgccaggtt ccacttgcgg atggcccgca tatagatttc cggcagcggg
    22321 cggcccttga cccggatcgt ctgcggccta atggccagcg ccccgttctg gaaagacagg
    22381 cggaaggtcg cggtcccgtt caagtaacgc ccttttaatt gaggccaccc ggcttcttcc
    22441 agcggcacgc tcagttggct ttgcaactgc cctccagcga ggtggacata gaccttgccc
    22501 ttgaatccct gcaaccgcgg gtcgtcggcg atcagcgcgt tgatttcatc ggcgctcaac
    22561 gtcagcgccg gggcggggcg ctgctcgcgg agcgccgcct cgaacgccgc gcaccgccgc
    22621 tttaattggt tcttttcgct ccgcgacagc aacgcggcgg gcaattccgc cggctgggaa
    22681 tcggtgaagc gcagcatcgt cttccaggtg taatgcagcg ccaccagcaa gccgcccagc
    22741 accagtaaca gccccaccgc gccgacgagg cacccatgga aaaaacagcc ccggcggcgg
    22801 gcggcggcgg gggagccggt catcgggctt tcttatgaaa gcggagtttg ggcgggatcg
    22861 gcggcggcgt gggaatcttc ggcggtgccg cgcgctgaag gcgttccttt tcccggcgcc
    22921 acgcttcgta ggtggcagac accaccggcg cggccgccgc cggcggggcg ggtgcttccg
    22981 ggggcgtcaa cttggcttcc cactcctgga ctttagccgc cagccaatcc cccgcctgaa
    23041 cgagggtctc gccgatcttc tcttcgggga acttgaggcc cccaatctcc aggttggcga
    23101 gcggcttcag ggcatcgagc ggttgcccgg gatcggccaa agccgggctc gggcgcgggg
    23161 cggcggaatc gagcgtttga tagccgttta ccagccagtc aacctggccg gtctcgatgt
    23221 ccaccaataa tccatgcacg gggattttgg ggccgatcag ggggctctgg cgcacgatat
    23281 ccgccgcctt gatgacgttg gcccgctcgc tgccgaacaa accgaagaac tcgttgagat
    23341 tgggcggcaa ttggtgccgt tccactccca ggcttcggaa ctgatccagg agcgccaggg
    23401 cggtggtttg gcccatcggg caatcggtgt ggccgatgat ggcaatctcc tggccgccct
    23461 cgacggcgca cgccagcgcc agcgaccgca cggtgctgcc cagggggtcg gtgatgatgt
    23521 ttcccgcgtt gcgcagccag atgaattggt cctcgggcac gcccagtact tcgggcaaca
    23581 gcggattgag ccgcacatcc aggcatgtca aagcgacgat gggcagttcg cttgcaaagt
    23641 ccgccgggcg caggccggcg ctcggatcac ccgccaccgc gcggtggttg gcgtcaatca
    23701 tggcttcaaa caggcgcata gccgtcgccg gcgaaccgtc cctacccgcg ggctttcggg
    23761 cggcccacaa agaaatagat cagcgcaccc agaacctgca ccaacacgat aacgagcacc
    23821 caaatgatct tctccgtgtc gcgcaaccct ttattcctga tggcgtgaat gagcatccac
    23881 acccagaaca caaaacaggc cagccccaaa agccccacca tcaaccccac aatccccgca
    23941 cccgccaaaa gcgatcctaa atcatccata gacggccttc gttacttcgg tttgtcctcg
    24001 cctttctccg cccccttctt tagcccgggc acctgttcga gcagcttctc gaacttgatg
    24061 cccgtaaggc tctcgatgac cggcggaagc tgcgcgacaa tctgggtcac gtcccccgtc
    24121 agcttgctgg cgccgccgcc cgcagcaggg ccggagttga tgatgaccat cttctcggtc
    24181 ttggccagcg gctcgctgat cttgccggcc acctccggca gcacgcggat gatcatctcg
    24241 accaccgccg cctcgttgta ttgcttgaag gattcggcct tgacgcgcat cgcctcggcg
    24301 gttgccttgc cttgcgcctc gatgacggcg gcctcggcca ggccgcgcgc cttgttggca
    24361 tccgcttccg cggtgccggt agctttgacc acatcggcgg cggcaaaccc ggttgccttg
    24421 gccgcggacg ccgctccggc ggcctcggtc tccaactgga acttgcgggc gttggccagg
    24481 gtttccacct tgtagcgctc ggcgtcggcg ggcttttgca cattggcctc cagttcccgc
    24541 tgtttgcgca ggatttcctg ttgctgcagc tcaatctgct tctgcttttc cacgatgttg
    24601 acctgcacct cttcggcctt caccaactgg ccggtcttgt atttctgcag gtcataagcc
    24661 aggtccgcct cggccttttt ctggttcacc gccgcctggt attgcgcgac gtttgtttga
    24721 taatcccgct gcgcctcggc gatcttggtg tcagccagga atttcgcctc ctgcccggcc
    24781 tggttcgcct gcgccgagcg gatggtcgcg tcgcggtcgg cctcggcctg cgcgataatg
    24841 gcatcccgtt tcacctgcgc gatgcgtggc ttgcccagcg cgtccaaata gccctggctg
    24901 tccttgatgt cgcggatggt gaaactgaca atgcccagtc ccatgttggc catatcgccc
    24961 gccgcgacct cctgcacctt ttgggcgaag gcgtcgcggt tctggtaaat atcttccacc
    25021 gtcatcgtgc ccaggatcgc gcgcaagtgg ccctcgagcg tttgcatggc gatgttttta
    25081 acggccgcgg tgtccttgct caagaactgc tccgccgccg tggcgatgga aatatcgtcg
    25141 cccttcacct tgatctgcgc cacgccgtcc acgcgcaccg gcacgccttt gctggtatag
    25201 acttcctgct cctgcttcac gtcaatggtc agcaactcca gcgaaagacg gtccaccttc
    25261 tcaataaccg gaatgacaaa cgtgccgccg cccttccgca cccggaaacc gcgcgtctgc
    25321 accatcccat ccgggtccac ataccgatgc cggcggccgg aaacgacgag cacctcgttc
    25381 gggccaacct tgacatagcg gctggcccag atcgccgcga agataacaat gacaaaaacc
    25441 agccccacca ccaccgaggc gaccgtgccg cccaggctgg cttccgccag cagaatggaa
    25501 tagttcgaca taagcaaaaa gttaattgca ggttatgggt tgcggaatgg aatcaaagag
    25561 gcgcgacata aaactgggtg ccgacgatgt gaacaatctt caccgcctgg ccgctggcga
    25621 ccgggacccc gctctcggtg cgcgccggcg cggtataacg cgaaccggcc tgcacatacg
    25681 cgatttcccc cacgcccccg gctggaatgg gcgtgatgac cgtggcgctc attccgacca
    25741 gcgttgccac gcgcaattcg ctggaggcat ccaccttgtg aaaaatcgtc ccgaacagcc
    25801 acaccacccc ggccgcgatg gccaacgcgc ccagcaaggc caatggggcg ctcacccagg
    25861 ggctccgggt ggcttccacc cgggcaaaaa tcatccccag cccgccaaac gcggtgataa
    25921 aagcggtgat cgtcgtgggg ctgaacggcg aaagccctgg aacgcccgcg tcttgaaagc
    25981 ccgcctcggc atgtccgccc gtccccacat ccgcatccac atcgtgcccg ccaaacaagt
    26041 ggcccaggag cgcgctgact acggcgaaca acagcccggc tccaaaacag accgaataca
    26101 ctaaaaagtc catagcccct cgctagtttg cacgcagtat aaaaccatga atccgccgga
    26161 ggcgcaagcc gcaaccttgc ccccgccgcc gccggccggc aatcgccgct cgccctcctc
    26221 ccgaggttgc ccgcactgcc ccggctcatc tatggaacgt cgtttcatct gcgcccctgg
    26281 agcaaacacc aaccgcgccc gctttgcact tataattttc cggccgcttt ataaacgcca
    26341 acccggccgc cgggaggtct cccggcgccg ccccgcgcct tttcatttga cgccccgtcc
    26401 gagccgggcg atagtgggct agcagccgga ttgaagcgtc ttcttccaca gcgaatgggc
    26461 tggtggcaga aacacccaga acggcgggcg tctgccgggg ggcctcggga ccccggacga
    26521 aaccaccaaa cgcacagaaa ggatattgct ttgtgagccg aagatcgaaa aaaccgaaga
    26581 tgcagacttt ccgctacgtc gcgccagcgg cgaccagcgt gctgctggtt ggggatttca
    26641 ccgaatggca gcagaacgcc ctggtcatgg agaagggagc cgacggagcc tgggccatca
    26701 gtgtcaagct gcccccgggc aagcatagct atctgttcat cgtggatggc aaatggtgcg
    26761 aggacccgga atgcaccgtg cgcgtgccca atccctttgg cggccatgat atggtccgcg
    26821 aagtgggctg aacttcgccg gttcgccgga ccgcggcgtt aacgccccca aggcggccaa
    26881 ccgggtttcc ccggcccggc ggccatcgtt cctcccccag gttgtcggcg gacgcaaaac
    26941 gcccgctgcc gggcgcctga cacgcccgtt ccaaccgccg cagcaaccac cccggctggc
    27001 aacgcgggcg cccgcttcag aagaccaccc ggcaagcgaa cccggttcgc gctcgcccga
    27061 gccggaaaac cgcgtatttc aggctggctc ctggcgccgt tttctgccct aatctgccgc
    27121 ctgatatgac gcattgtcca accgttccga acccgccgaa ccgccgcctc tgccgcgccc
    27181 ggccgcgaat ctgacccttg ctttccgtcc cccgagcgcc cccatgaaac agctcatcct
    27241 gattgattac gccattattg gcgtctatgt gctggcctcc tttgccatcg gcaactacta
    27301 cctgcggcga gccggctctt ccctggacga atacttcctg tccggtcgca agacctcctg
    27361 gtggctggtc gcattctcga tggcggccac gaactactcg attgattacc cgctggccat
    27421 ttgcaagctg gtggccaaat acggcatcat gggcacctgg tatgtgtggt cgctggccat
    27481 ggccgccgtg gccaccacct tcttcttttc ccgtttgtgg cgccgggccc gggtggtgac
    27541 cgacgccgag ttgatcaaat accggtatag cggaaacatc ggcaatgccc tgcgcatctt
    27601 caagagcatc tacttcggcg tcatcatcaa ctgcttcgtc ctgggctggg tcttccgggc
    27661 gctgaccaaa gtgatgaccg tcctgactcc ttggaatcaa tggtacgtcc tgacctttgc
    27721 cgtggccgtc accgtgatct acacgatcag cggcgggctt tgcggcgtga tcatgaccga
    27781 cttcgtccag tatgtcatca tcatcatcgg cgcggtggcg ctggccgtct atggcgtcgt
    27841 gcatgtcggc ggcctcgcgg ccatgtccgc caaaatcgag gccctgtacc cgggcaaaca
    27901 cttcctggca ttctcgcccg ggttcaccgg cggccagttg atgccctggt cggcctttct
    27961 ggtttacctg ttcatccaat ggtgggccaa caagtattcc gacggcggcg gcaaacatat
    28021 ccagcgcatg agcgccgccc ggaacgaagt ccactccgtc ctgggcacgt tcgcctattc
    28081 gctcctttat gtcctttcca cctggccgat catcatcacg gcgctgtgcg cgctgatcct
    28141 cttcggcaag ctgcccgacc cggagctggg ctatccgcgg ctgatgagcg agctcctgcc
    28201 caacggcgtc ctggggcttt gcgtcgttgg cttgctgggg gcgttcatgt ccaccatttc
    28261 cacccacttc aacctggggc cctcctacct catcaacgac atctacaagg ctttcctggt
    28321 gaagaatgcc tccgagaaac actatatcct cgtctcccgc tgcgccacgg gcctggtcgc
    28381 cctgaccggc atgattctca gcgtcttcat cgagtccatt gcggacatgt ggcagttcgt
    28441 tttgtccttc gccagcggcg ccggcattat ctggatactg cgctggttct ggtggcgcat
    28501 caatgcctgg tccgagtttt cctcgatgat ctgcagcgcc atcgtggcca cctacctgaa
    28561 gcaggcgcac ccggagatcc cgtttgaaag cgccctgctg atcatcatcg ccatcaccgt
    28621 ccccgtggcg ctgctggtca ccttcctaac cccgccggtc caggacgaaa ccctgcggga
    28681 cttctaccag cgcgtgcggc cggggcgctg gggctggcgc cgcatcgcgg cgaaatacgg
    28741 catcccgcgc tcgccctacc tggccaaggc cgttgtgaac ctgctgttgg ggttggcgct
    28801 gctgttcctg gtcaacttcg gcgtcggcac cttgctgctg cgctcgcgct ggctcgggat
    28861 cgccgaactg ttggcgggcg ccctcctggc actcgtgctg attaagcgaa tccgggaaga
    28921 ggaacaactc ccggaccccc aggattccgc cccgccctcg ccgggccagg ggattgtgta
    28981 aggctaatcc ttacaaaaaa atcagccggg cgcttacaga caaccacggg accttctggt
    29041 aaaagtagtg cccgcaatgg acagcgcaag cggcgggccg ccccgttgcc gaagcgcgga
    29101 ccgcccggct ggctgttgca gcggcagccc ggttgagcag gtgggtttgg gtgctcatca
    29161 ttagcccggt gcaactcaga tttcctcccg gtcccgggct gccgcttcag cagcctttgc
    29221 gcaaaccccc accggccccc gccggcgcta gttgcgagag ttccctttca ccaactgcgc
    29281 ctcatgggtc tgcaaatact tctccatcgt ctcgatactg ctggtctgaa tccggcgcgc
    29341 ctgccccaat tcccgcatca tgcggtagtc cagcagccgc ttgcctagct ccacaatctg
    29401 gatatacgcc ttgtccgtct tccacaactg cccattggcc agcgccggcc gccgcttctt
    29461 cctattcgac tttattttca cgcctattag acgcgcgaaa cccctgtttt gttcagattt
    29521 tagtcggcca aagtttgccg cggcgctccg ggccccgggc ttgcgcctcc cggcgcgccc
    29581 gcattcccgt ttaccccgcc ccggcgcgtc tggtaaactc cgggcatggc gtttgattca
    29641 ttacgcgact ttctcgggca tttggaggcg gccggcgagc tgcggcgcct cacgcaaccg
    29701 gtggccagcg aactggagat taccgaactg gccgaccgcg aaatgaagaa gcccggcggc
    29761 ggccaggccc tgttggtgga gaagccgacc gtcaacgggc aaatctcgcc cttcccgctc
    29821 gccatcaacg cgctgggctc gtatcggcgc atggcgctga gcctgggcgc cccctcgatc
    29881 gaggaagccg ccgccgcgct cggcgccctc gtgaaagcca aaccgcccgc cagcttcaaa
    29941 gaggctctcc aattgctcgg caccgcgttc gacctccgcc acgcccggcc gaagcgcgtc
    30001 aaagacggcg cctgccagga agtcatccac cacttcgacg ctcccccgcc gcgcaccgcg
    30061 ccgtggcccc cggcccccga ttggcgcacc cccgccgcgt tcgacccccg cccgcccacc
    30121 ctgctgagcc tccccatcca gcaatgctgg ccgctggacg gcggacgctt cattacgctg
    30181 ccctgcgtgg tgacccgcga ccccgacacc ggcgcgcgca acgtgggcat gtaccgcatg
    30241 caggtctatg acgagcgcac caccgggctg cattggcagt tgcagaaagt cgccgcccgc
    30301 cacggccgcc gctactacga aaccggcgcg cgcatgccgg tggccgtctt cctgggcggc
    30361 gacccggtgt acaccttctg cgccaccgcc ccgctgccgg acgggctgga tgagttcctg
    30421 ctggccggct acctgcgcca gaaatccgcc gaactggttc gctgccagac ctgcgacctg
    30481 gaagtgcccg ccgacgcgga ctttgtcctg gagggatacg tggaccccgc cgaaccgctg
    30541 cgcgaggaag gtccattcgg cgaccatacc ggcttttact cgctgcccga ccgctacccg
    30601 gtcttccacc tcaccgccat cacccaccgc cagcgggcga tctacccggc caccatcgtc
    30661 ggcccgcccc cgatggagga ttattatatc ggcagcgcct ccgtaaaact attcctgccc
    30721 atcctcaaaa tgaccttccc ggaactggtg gacctggccc tgcccgccga gggcgtcttc
    30781 cacaacctgg tcttcgtcag catcaaaaag acctacccca tgcaggcctt caaaatcatg
    30841 cacggcctct ggggcatggg gcaaatgatg ttcgccaaat atatcgtggt cgtggatgac
    30901 gacgtggacg tgcataactc ccgcgaagtc ctctttcacc tctgcgccaa caccgacccg
    30961 caacgcgacg cccttttcac caaaggcccc gccgacgtgc ttgaccacgc cacgcccgtg
    31021 ctgggcgtcg gctccaaaat gggcatggac gccacgcgca agctccccgg cgaaggccac
    31081 ccgcgcgact ggccgccgct catccagatg ccggaagcgg tgcggcagaa aatccgctcg
    31141 atgctggcgt agccgccggc cccgggaaaa gaaccccgcg cgccgggagc cggcgccggg
    31201 ccgccggata aactctctcc aacatcccgc ggcctcaatg gccagcgacg gccctccgcg
    31261 cgcccgccca caacagggtg gacgcttcgg acggcgcttg ctaagctcgc tccatgcctg
    31321 gtgaactcga acgccaattc gccattaccc gcgcagggca gcgccccggc gaacacccca
    31381 aacaccgcgc cccatgaaac ccggtgcccg gaaaccgctc cgaagccggg agcacccgca
    31441 catgcagccg caaatcgccg ggcaaccgct cccgcaccgg ctcaaccgcc gcgactttct
    31501 gcgcggcgcc gcggcgggaa tggccctggc cttcctgccc ccggcggcgg cggcgcccgc
    31561 cggcccggtg cgcatcgaaa cccctttcca cggcgccatt ctcaacccgc ggcaaggccg
    31621 cgccgttaaa cgcggcctcg ccatccgcgt gagcggcacc gcccccgccg ccatgcgggt
    31681 caaagtcaac gggcaaacag cgcgccgaga gggcgaccgc ttcgacgccg aggtcatcct
    31741 tcgccagccg gtgacggaga ttgccgccgt cgccggagca tcggccgccg gcggcgaaga
    31801 ccgcatccgg gtggtctggg accggcactc gcgcccccgc taccacttcg ccctggatga
    31861 caacatttat tttctgcgcg acatcgcccg gcagcgctat gactcccttt tcgattgcgc
    31921 ctacctcaaa ctgctgcgga acctgaacct caaatacggc acccgcttct ccgtcaatct
    31981 ctacttcgcg gcggaggatg gctttacgct ggccgagttc ccggaccgct accgcggcca
    32041 gtggcaggac aacgcgcact ggctgaaact ggcctttcac gcctacgccg acaagcccga
    32101 ccggccctac caaaatgccc cggcggaaaa gctcctggcc gattacgacc aggtcgccgc
    32161 gcagatccat cggttcgccg gcgccgccac cttcgccccg cccaccaacc tccactggtc
    32221 catggcgcgc ccggacgccc tccgcgccct ggcccggcgc ggcgtgcggt gcctgagcgg
    32281 ctatttccgc aaagacaacg gcggctggga catcaattat gggctggacg accgccggtc
    32341 ggaatacctg tccaaacacg acctcctgat ggacttcgag accgggatca tctttttccg
    32401 cgacgccatc gtctgcaaca cggtgacccc ggaccaggcc gcccaggtcc tggctccgct
    32461 ggcggccggc ccggaacgcc gcgaccccat caacctgctg tgccacgaac agtacttctg
    32521 gcccttttat ccggcgtacc aacccgacta cgcccagcgg atcgaggccg ccatccgctg
    32581 gacaacggag cacggctacc aaccctcctt tttccacgaa ggcatcctgg gcagcccgga
    32641 ggcgtgaacg cgccggggcg cgcggcggct tccccgccgg aagcggcccg ctcacggccg
    32701 ccgccgctca ccggcgaggt actccagcag gtccgccatg tccggcagcg agagcccctc
    32761 ggccagcgcc tcgggcatga gcgaccccgg cagcggcgcc agatgccgca ggtttagacg
    32821 cggcagaacc accggctggc cgttcgcctg ctgcagggtc accgccgcca ggtgatcgtc
    32881 gcgcagccgg ccccagaaca attcgcccgc ggcggtccgg gccaggtaca actcattgcc
    32941 gggccggatc tccgcgctcg gctccagcat cgccgccagc gcccattcct tgccgcgcat
    33001 cggcgccgtg gccagctccg gccccaggcc cggcccggcc ggccccggct gatggcacac
    33061 cgcgcaatac gcgagaaaaa ccccccgtcc gcggtccgcc gcgccccgca cgttcaacgc
    33121 cggctggaaa tgctgcagcg cctccggccg ccgcgcccgc agcggcccca gcagccgcgc
    33181 cgcgcgttcg gcaatcgccg gctcgggata cgcgcgcagc agggtcactt ggtcatccgt
    33241 gagatcggcc aggcccaggc gcccctcttc cagcgcgtcc agcacgacgc ccaggcggtc
    33301 ggcgcgggcc agcagcgcgg cggcggcggc ggcgcgcaag gccggcgtca aggcccgcca
    33361 ccggcgcagc agcgcgtcgg gcagccgcgg atcgtcgtag ctgcccaggg tggccagggc
    33421 ggcggagcga agcgcttccg attgccgcga ccccgccagc agcagcaggt gatccgccgt
    33481 ttcaagatag gtataggggc aggagggcaa cagccggatc gccccaagcc gcaccggttc
    33541 cggaaactgg acgttcggca tgaccgtcag ggcttccgcg tagaagcggg gcagcggccc
    33601 ctgcgggttc atccgggcca gcgaaatacc cgcccggcgc aggccttcgc tcatcgccgc
    33661 cagcgtcggc aaagcccgcg ccgagtccgg cggcgtctgg tccgcaaacg caagcacccg
    33721 ggcaatttcc tccggctgtc cctgcgcccc gatcatcgcc gccagccggt ccagcagttc
    33781 ctgcccggcg gccgagtccc gcaccgcccg ctgccgggcc agggcaatca agacttccgc
    33841 cgccccctcg cccagcgagc tcaaaaccgc cgcctgccgc cagggatcgt cgcccgcctc
    33901 ccacagcagc tccgccagca ccggcgcgcg atccggcgcc cggatttccc ccagggtcag
    33961 cgcgagctga tagcgcacgc ggagcgaagg gtcggccgcc agcgatttaa gctgccccca
    34021 gatcggcgcc ggcagcgccc cgcgccgggc ccgcgcctcg gccagccgca ccgcctgttc
    34081 ccgcacccgc gcatccggat ctttcaacgc ccggagcaac ggctccgcgc tcaacgcgcc
    34141 gagcccctcg agcgcgtaca gcgcctgcgc gcgcgtcagc ggagcccggg agtaataaaa
    34201 caatttcgcc agggccggcg ccgccgccgg atcctgccgc tcgtagagca gccgcgcggc
    34261 cgtgtcgcga tgccacccgt tcggatgcgc gagagccgcc accagctcgg ccgtggtggc
    34321 ccgccccagc cggacggctt tgggcggctt gaaatcctgc ggcacaatgc ggtaaatccg
    34381 cccgcccgcg cgatcggcgg gcgccgccac aacgtaaagc gcgccgtccg gcccattggc
    34441 aacctgcacc ggccgaaacg ccggatcggc ggacatcaag aactcgccgc gcgcatcgtc
    34501 cggagcccgc tccgccatca gccgcagccc cgcctcgcgc agcaccaccc ggtgaatgac
    34561 ccgggccgac gcgtcggcaa tgaacacgtt gccctgaaag ttcgtgggaa acgcgtggcc
    34621 gcgataaaca acgcagcttc gcgcattcgt cagccaggcg gccgtgggcg cgttcgtggc
    34681 caggaacgcc gccgcgggcg gcgcggcgtc cccgggcggg agctggaaga cggtcgcggc
    34741 cgggccgagc acatcggcca cctcgggagg cgccgggaaa aacgggttgc gctcccggta
    34801 gcgcagcgga aacagcgcct gccgcagcgg acgcgccgga tcgcacagca gcgcccggcc
    34861 ccagttgtcc aaagccaggc cggcttccgc cccgcctccg ccgacggcga gcgccagccc
    34921 gcgcggggcg aaggaaaaac accggctcgg caacgccgga tccgtgccca ccccggcggc
    34981 atgaatatgg ttgtccaggc cccaggtgaa gctgtgcggg gccatccggg ccgccagcgc
    35041 attggtgctt tcaaacccgc tgaagacctc cctgcgcacc gtggcgatgc cgttgctccc
    35101 ggcatccttc aggtaaagaa tggcggcgcc ggcggccacg aacactccgc ccccataaca
    35161 cgccaccgcc gagaccgccg ccagcttgtc cgcatacacc cggctcgacc ggaattggcc
    35221 ggtgccgtcg gggtcttcca gcaaccgaac gcgccccacc gccccgccgt tgtcgccggc
    35281 gtcgccgtcg gccagcgtct ccgctacgaa caaccggccg ttctcgtcaa acgcaatggc
    35341 cgccggcgcg gcccccggcg cttcggcggc ggccagttcc aggcggaacc cggcctgaac
    35401 ccgaaacccg gccgggatat tcgtaagctg gctcgaccaa ccggcgggcg gggcgttgga
    35461 ctccgtttcc gccgcggccg cccaacccgg ccccgccagc agcaacgcca gcatgagcca
    35521 ccgccccgcc agcggccccg gcgcgagccg gcagcccgcg ccaccgggca cccggcccgg
    35581 agcccggccg cccgggcgcg tcccccgcga ccgcccgcgc ccgcgccgcg aaaatccttt
    35641 aacgtcattt tgcaccgtca gcggttgtcc atgaaacagt tgcatcctgg ccgcgcggaa
    35701 aacccgccgg ccgcgcgcct tcgctcgcac tcccagtttt atccaccccc gcccgcttgg
    35761 aaaggcaaaa ccccgccgcc cgaatcgttc ccccggccgc ggccggcggg catccgcctc
    35821 ccccgcccgg ctggcggcgc gctgccggct ggacaacccg gaacggttaa cccatcttca
    35881 ccgcgtggcc ggttgggccg cctttgattc ggactgcgct ttatggaaga acctttgctg
    35941 attccggaac ccttgccgga agctccgccg ccgcccgcga tgccgctcgc ggcgcggttg
    36001 ctgaatgtct ttgccatccc gggcgcggtg ttcgcggacg tcagggcctg ccgcgggtcc
    36061 gccgccaatt ggatcgtgcc cgcgctgctg ctgggattgg ccggcatgct caccgcgatg
    36121 gccgtgctct cccagccgga ctttcagcag cagatgaaca aacgcttcgc ggaacaggcc
    36181 caggcgctgg aacagcaagt caaggcgggc aagatcaaac cggcggaagc cgagcgcctg
    36241 cgggccttta cccgaacctt cgcccggccg gaggtgctcc ggtccctggg cggaaccatg
    36301 gccttcgggt tcggcgtcgt ccgcgtgttc tggtgggcgt tggtgctgtg gctgcttggg
    36361 cgggcgttcc tgaaaatcca cgtgaactat ctcaaggcgc tcgaagttgc gggactggcc
    36421 ttgatgatca gcgtgctggg ctcggtggtg tcgctgctgc tgatggtcaa cctgccccgg
    36481 ctgttttcca cccaggactg gagccaggcc gtcagcgact tcaaccccag ccataagaac
    36541 tatctgctgc tgggaacggc caatatattc gccctctggc tggtttgcgt gctctccgtc
    36601 ggcctggccc ggctggccga cgtgcccttc cgacgggcga tgtggtttgt gttcgccgcc
    36661 tggctgatcc agcaactctg cctggtgacg ctgcaagggg tgaccgggca attcatgtta
    36721 tagcccgccc cccggcccgc gaaccacccc gaaacgacgg gagcctcccc catgaagccg
    36781 gctggttgaa ggccggcaga cacgaaaaag gacctctctc aaccatctcc tggcatcgcc
    36841 atgaactgtc caatttttgg acactcccgg aaacattcgc cttaatctcc cttccccacc
    36901 ccagttccgt tccccaattg ccacccgcca atttccctaa taaaaagccc tcatttggct
    36961 agcaataggc cgtgtacaag gggtgtacaa gggatgctca ccggatgcgc ataggaaaag
    37021 ctgatattca cccggcgagc atcccttgta caccccttgt acacggccta ttgggagggg
    37081 gaaatgatgg ggaagcgtcg gaaatgagtg gacttgttgg aggtggattg attgcgaagg
    37141 cagcggcgag ttggcgcacg aataaagggt gtccgggacg ggaggccgcc agctatttat
    37201 agcggagccg gttgaggggc ttgctgccgt tgccgctgtg gaggtgcctc aggggaaaga
    37261 gggtcgcggc ctccagccgc cttgattggg tcaggatggc gtggcgggct tggctccata
    37321 cggcgcgggt ctctgtcccg ccgctgaata agccggttaa cgtgagggaa tgggcgccgt
    37381 ccccggccgg ggtgttgccg taacggacgg cgggtcggcg cgcggaaggc ggccgtgaat
    37441 gcgggtccgg gatttctgtt gggggggcag actcatgttt gtggcttcgg caaccctgaa
    37501 cttgattcaa cgcagcggcg agtgccgcgt ccacctcccc gccccggtgg catttatccg
    37561 gaatcaatgc gcggggcagc cgcctgcgtc taatgagttg ctgctgccgc gcccctgcgg
    37621 cagactattg gcggatgtct attgccagga tgccccggtg gtccgtgcgc caggaacagg
    37681 ccgaagtctc gcgcctggct tgggcctttg tcttttccct ggcgcttcac ctggcggtgt
    37741 tcggcggtta tcgcaccggc cagaagctgc atttatggca aaaccttcac tggcccgcct
    37801 ggctgcagcc gcctcgcgcg ctgacggaac tcatccggaa gaaagaaaag cctccgccgg
    37861 cgcggacgca ggagcctccc ctgatgttcg tgaatgtcag ccccgcccag gccacggcgg
    37921 aaccgcccaa gaccgcgcga tattattccg acaagaacgc gcacgcggcc aaccccgacg
    37981 cggataagat cacggacgtg cccaggatca ccggccggca gacgcaagtt ccccggacgg
    38041 aggatatccc gcgcccgaag tttgtccctt tgcagccggc gccggccgcg caaacgccgc
    38101 aggaggcgca accggaggtt cgccccaaac ccgccgatac tcccggggac ctggtgctgg
    38161 ccaagccgtc cccaacgccc cgcctcacgg acggcgacgc ggcaacggcc cagccggccc
    38221 ggccgcgaac cctccgggaa gcccgggccc ggttggcgga accgcgggcg gccggtgaaa
    38281 tgatgaagca ggagggcggg gtcagccgcc gccacgccat cgcttcgctg gacacccggg
    38341 ccactccctt tggcgcctat gacgccgcgc tggtggcggc catttcgcag cgctggtaca
    38401 ccttgctgga tcagcgcgag tatgcgtccg atcggcgcgg caaggtggtg ctgcagtttc
    38461 gcctgcatca taacgggcgc gtgacggata tgaccgtggc ggagaacacc gtgggcgagg
    38521 tgctggggct gctctgccag aaggcggtgc tggatccggc gccctttgcc agttggccga
    38581 gcgatatgcg ccgcacgctg ggagacacgc gcaatatcca attcactttt tattacaatt
    38641 aatggtctat ggaatccctg gtttacatcc tgctctttat cacgtcggtg gtgggcctga
    38701 ccttcatcgt cgagcgcggc attgtcctgc gctggcggcg cgtcgtgccc gccgaggtcg
    38761 aggccgttgt ccaggcctgc cagtcgcgcg cggacgtgcc gaacctccgc cgcgtctgcc
    38821 agcaacataa atcccccacc agccggctgc tgttgattgg cgccgagcac ctggactggc
    38881 cccgggcgga aatcaccgac gccattcaga cccgcgcgcg caatgaggtg gcccgcctgg
    38941 agcgcgggct ggtggtgctg gagatcatag tcggcatcgc gccgttgctg ggcctggtgg
    39001 gcaccatttt gggcatgatg accgcgttcg gggatgtcgg gcaggggggc cagattgatc
    39061 cggccgaact ggccaagggc atttcgctga ttttgcgcgc cacgctcttt ggcctgctga
    39121 ttgccattcc ggccctggtc ttttggagtt attacaccaa gaaagtggaa tccctggccc
    39181 tcgagctgga gtcattgtgc gacgagttcc tccgccgcct ctaccgcgag gaagagccgc
    39241 ttccccgggg gaaaagctaa ccgccgcccc ggcgaatctt cgcttcccga tatggccttg
    39301 cgattttaca cccgaaaacg gcgggaagtc ccgacggtga tcattgtcgc gctgatagac
    39361 atcctcatcg tcctggtgat cttcctgatg gtgaccacga cgttcaagca gcagcccgcg
    39421 ctcaagctcg ctttgcccga atcctcccag gcccaaaaga ccggcgccag cgaagccccg
    39481 cccctggtcg tcagcattga tccccagggc cagctccgcc tgggcgccga cgcccggccc
    39541 gtcaccctcg agcgcctccg gcaagagctt ctcggggccg tcgccaaaac cccggaactc
    39601 cggctcgccc tgagcgccga taaaatggcc cctttcgggc aggtggtgaa agtgatggac
    39661 gttgccaaag aagccaacct caaggtcgtc aatgcctaca ccaaggaaac cggcaagccc
    39721 tgatccagcc gccgcgcggc tccggcgttt cgcctctgga tcggcaggcg cgcgcgggaa
    39781 aggccggctt tggcgattcc gccgcgccct gtccgcctgc ggcgcggcgc tggggctggc
    39841 ggtttcgccc gcgggcggcc agccgccgcc cgggctggcg gcgcaaaagt ccggcgggga
    39901 cgtggtcttg agcctgacgg gcgctcccgg cagcggcggc acgattcaat atcgctccgg
    39961 gctttccgcc tcgaacccct ggcactacct ggcccacttt gcggcattgt ccgccagccc
    40021 ctgccaggtg acggaaccgg gcgcgctcca cgccgcgcgg ttttaccggg cgttttctta
    40081 ttcactgccc acgaacgtgg tgccgcgctc gaacatggtt tgggttgcgc cgggggtgtt
    40141 tgtcatgggc agcccggcgg gcgaggcgct tcgcgccgcg aatgaaaccc agcatacggt
    40201 gacgctgacc cgggggtttt acatgaacca gtacgaggtg acgcagggag agtacctggc
    40261 gctgatgggg accaacccca gccggttcac cacccaggac tataatggaa acccgatcag
    40321 cccggatttg aaccgccccg tcgaacaggt gagctggatt gacgcgacca actattgcgg
    40381 gcagttgacg agccaggagc ggaccgccgg gcggttgccg gcgggctgga ggtatcgcct
    40441 gccgaccgag gccgaatggg agtatgcgtg ccgggcggga acggccaccg ccttcagctt
    40501 cggcaacgcg attcgcggcg gagcggccaa cttccaaaac cactacgagt atgacgcggg
    40561 cattggggat ctctacgtgc ccggcccgga ggttccgtgg cgggcatgca caacggccgt
    40621 gggcagctac ccgccgaatg cgtgggggct gtatgacctg cacgggaatg tctgggaatg
    40681 gtgccaggat tggctcggaa cctacccggc ggggagcgtg actgatcccc agggcccggc
    40741 ctccggctcc gggcgcgtgt atcgcggcgg cagttgggat tattggggcc gctacagccg
    40801 gtccgcctgc cgcttttcgc tcgccccgtc caaacagtac gactttatcg ggttccgggt
    40861 tgtcctggcc ccggatccgt aggccgcgcg ggcttaagcc ttcaggcacg ccccggccac
    40921 ccgctcgatt tgggcatcgg tgagttccgg gaacatgggc agggaaatga ttttgccgac
    40981 cagcgcttcg gtgacgggga agctgccggg cgggtagccc aactcggcga aggcgggctg
    41041 caaatgcacg ggaatcgggt agtgaatccc gtgggaaatg ttggcggcgt cgaacgcggc
    41101 ttgctgtttt gcccgatcgg gcacctgcac cacgtagagg tggaaaaccg gtttggcata
    41161 cggcgccacc gggggcgtga acaggccagt gccagccagc agccggtcgt acttggccgc
    41221 ggcctggcgg cgcgcttcgt tccactgctc caaccggcgc agcttcacgc gcagcacgac
    41281 ggcttgcagg gtgtccagcc ggctgttgaa tcctttgatc tggtgctcgt atttgacctt
    41341 ctggccgtga ttgcgcagcg tccggacctg ttcggccagg tcggcccggg tcgtggtcac
    41401 cgctccgccg tcgccgtagg cacccaggtt tttgcccggg tagaagctga acgccgcaat
    41461 gtctcccatc gagccgcagc gccgcctttt atactcggcg ccgtgcgcct ggcaggcgtc
    41521 ctcgatcaca tacagcttat gccgccgcgc aatctccagg agggggtcca tgtccgccgg
    41581 ctggccataa agatgcacgg gcatgattgc cttggtgcgg ggagtgatcg cccgctccac
    41641 ctgcgccggg tctatcgtgt aaaaccgggg gtcacagtcc accaggacgg cccgggcgcc
    41701 cgcctggtca atggcggaca ccgtggcaat gaacgtatgg gtgcaggtga tgacctcgtc
    41761 gcccgcgccg atgccgcacg ccagcagcgc cagtttcagc gcctccgtgc cgttggccac
    41821 ggccacgctg tgcggagccc ggcagaacgc cgcaaactcc tgctcgaaga gcttgacgtc
    41881 ctcgcccagg acgaaatctc cgcgcgcgca cacctttgcc atggccgcat ccacctccgt
    41941 tcggagctgc tgatactgcg ccactaaatc cacgaattgt atcttttcgt tgttcatacc
    42001 aagcttctgc aagttaatac ccggcgttta cgccgcttct tcttagccca attcgcaagc
    42061 cgcctcaagg tctttttccc gctccgggct tgctttgcgg ccgggagcgg cggaagctgt
    42121 gggccgcaac gattatggcc aaaaaagcgt tcattaccgg cattacgggg caggacggtt
    42181 cctacctggc ggagctgctc ttgagcaagg gctacgaggt gcatgggctg atccggcggg
    42241 cgagcacctt caacaccgcg cggctggacc ccatttatga cgatccccat tgcgacgcgc
    42301 gccgtttatt tctgcactat ggcgacttga gcgacgccag cgcgctggcg aggctaatcg
    42361 gcaaaatcca gccggaggag gtttacaacc tggcggccca gagccacgtg cgggtgagct
    42421 ttgacagccc cgaatacacg gcggacatca cggcgaccgg cacggtgcgg ctgctggagg
    42481 ccattcgcga gacgggcatc cgcccgcgct tttaccaggc ttcttcgagc gagatgttcg
    42541 gcctggtgca ggaggttccg cagaccgagc gcacgccctt ttatccgcgc agcccgtatg
    42601 ggtgcgccaa ggtgttttcc tattgggcca ccgtcaacta ccgggaatcc tacgggctcc
    42661 atgccagcaa tggcatcctg ttcaaccacg aatcgccgcg gcgcggggag acctttgtga
    42721 cgcggaaaat cacacgggcc gtggcgcaca tcctggcggg attgcaagac aagctgtatc
    42781 taggcaacct cgaggcccgg cgcgattggg gctacgcccg ggagtacgtc gaagccatgt
    42841 ggctcatgct ccaacaggac aagccggatg attacgtgat cgccacgggc gaaacgcatt
    42901 cggttgacga gttcctcacg gccgcctttg cctacgccgg gctggaccgc gagaagcacg
    42961 tgcagattga cgcgcggtat taccggcccg ccgaggtgga cttgttgatc ggtgacgcga
    43021 gcaaagccag gcgccaactg gggtgggcgc ctaaaacccg gttcccggag ctggtaaaac
    43081 tcatggtgga tgccgatatc gagctgctcc gccagcgccg ccaggggcag gtccgcagca
    43141 accgggagcg ctgaatccag ccgggagcgg cccaccgcca acccctccga agacgccccc
    43201 catcgagccg cggcgcgcca aggtcagtcc ccgcggcggc cacgcaagcc cgtccagccc
    43261 ggcccggccc ggcgcgcctg cgatcgggcg gcgacggttc aagcgcggcg ccttgattca
    43321 gaagcggaag cttacgccaa aaacatgcag attcaggccc gggttgggcg cggccagccc
    43381 cgcgttggac atgtgctgcc agcggtagcc aagccgccaa tgcctggcca ggtccaaatt
    43441 caagccggcg tggcacgtaa attgtatggc cgcgccgaag tcgcgaagct caaaatcatg
    43501 gcggctgata tacgtggggc ttaccccggc ggtcagcgac aacgggcagc gctgccgatg
    43561 cagctccagg ctgggcccga cgcttccaat cacggcatta tatttgcggt cccccagcca
    43621 gcctcccgat aaatccagcc ggccctgcac gtcccatcct ttgcccagag accattccag
    43681 cggcagatgc caggtaccaa atacctgggc caggttgaaa ttccggcaac cctgattcgc
    43741 cggaatcccg ccccgcaccc ccaccgactc ccatcgaagc ccctcacccc gccccggata
    43801 actccagaac aaaagacaga ccatcaccca gttccccatg ccgatacggc ccaaagtctt
    43861 cctggtaatg ctcatgtcat tatatcctat gaggagacct ggctcggcac aagtttcaac
    43921 acggcacgga agaccgggcc gcgcgggttt gcgggaaagc cgcatcggca atccccggcc
    43981 gcccgcaccg ccttcgctaa cgcccgcttc cggcttttgg ccgcgaacgg aaaccggaga
    44041 ggcgctcgcc gcgccaaaac cggtcaaagc aggcttgcca tgcgaaaaag ggggtgctat
    44101 gcaaaccccc gatggattcc ggctcaaaca ggccttagac ccgcgtcatg gaaatcagta
    44161 ccattgggcg cccgcgcaat gtggggtggg ttcgggcagg ggcgttgctc tatggtgatt
    44221 ggggtgccag caaggcctac gtcattggtc tgggggttgc ggcggtgggt tttgcggcgc
    44281 tgccccactt gctggccgtc tgcctggtca cggccattgt tggcatcaac tacatctggg
    44341 tgtgccgctg ctttgcgaat gggggcgggg tttataccgc ggcgggcttg cactcgcggc
    44401 ggctggcgat ggcgggggga ttgctgttgt tggccgattt catcgtcacg gcctcgctga
    44461 gctgcctgga cgcttttcac taccttggct tcgaccaggt cgaagccaag aaatgggcca
    44521 tctccgccat cttccttatt ggcgccatga acttcttcgg gcccaagcac gcgggcagtc
    44581 tggcgatctg gctggcgctg ccggtcgttg ccgtggtgat cgtcctgatc ggcgccggcc
    44641 tgccgcacct gggtcagttc catgcccgcg cgccgtcggg agggctgctt cccaactggg
    44701 tggcgtttgt gggcatgatc ctcgccctct cgggcgtcga ggccgcggcc agcagcacgg
    44761 gggtcatgaa actggacccc ggcgccacgg tggacaagcc atccgtgcgc atagcctccc
    44821 ggcgcgcggt cacggcggtc atgctggagg tggttatcgc cacggctgcc ctcagtttgc
    44881 tggccatgtg cctgccggtg ggcgaggatc aactggcggc gcaccgggag gatatgctcc
    44941 ggttcatggg ggaaatcttt gtggggccgt ggtttggcaa ggtggtgggc tgggtcttcg
    45001 ccttgctgct cctgtcagcg gtgaacaccg ccatcggcgg catggtagcg ctgctgtatg
    45061 tcatggcgca tgatcgcgaa ctgccccaca ccttcgtcca gctcaaccgc tttggcgtgc
    45121 cctgggtcgc cctgatcgcc gccaccattc tgccggtcat cgtgctggac ctcaatgatt
    45181 cggtcgaagc cctgtccagc ctttacgcca tcggcgtggt cggcgccatt gtgctggacg
    45241 tcggcagttg cgcctttgcc cggacgctgc ccctgaccga ccggcagcgc ctggtcatga
    45301 agctgacgat cctgctcctg gcggcaatct gggtgaccat cgcgctccac aaaccgcggg
    45361 ccctggtttt cgtggtgatg gtgctggcgg tggggtttgc catccggcag cttgcccgcc
    45421 ggcatttcct gcccccggaa gtttccgcgc ccgccccggt ttccggcctc cgcccggtgt
    45481 ccggccaatt catcggccag ctcatcctgg tcgccgcccg cggctggact cccgccctcc
    45541 agttcgccct ggaagaaagc cgcgtgcgcg gctcccggct gctcgtgctc tatatccgcg
    45601 aaatcgcggt aaagacgggc atgagcggca actggcagaa agacccggcc gccaaggccc
    45661 tctttacccg cctcggggcc gaggctggcg acgtgcaggt cagcaagctc tacagcatca
    45721 gcgattcccc ggcggatacc atcattgaca ttgccgcgac gtttggcgtg gacaccgtcg
    45781 tgctcggcgg cagccgccgc gctgccctcg tccatctcct caaaggcaac gtcgtcgccc
    45841 gcgtcgcggc caacctgccc gagtccatgc acctgatagt catcgggtag cgcggcccgc
    45901 cctgccctga gccgtccgcc atggcgacga cccgctcttc cgccggcccg gcggctggcc
    45961 ccgcgccgcc agcccgggag cagacctgtc gcggcccgtt tgcgatcatg gtcctgcttt
    46021 tctttctctg ggggttcatg accgtatgca acgacctgct gatcccgcgc ttcaagtcgg
    46081 ccttcgcgtt gagctacttc caggcgatgc tggtgccgct ggccttcttc ggcgcttatt
    46141 tcaccggcgc gctgctctac tttctggcct ccgtcctcgc gggcgatccg cttgcgcgga
    46201 tggggcacca gaacggcgtg gtgctcgggc tgctgatttc agcggcgggc agcgcgctgt
    46261 tctggccggc ggcttccgtt ccggcgtatc cgctgtttct gctcgcgctc tttatcgtgg
    46321 gcctcggctt tgccgtgctg caaatcgccg ccaatcccta cgtcgccctc ctcggccccg
    46381 aacggacggc ggccagccgg ttgaacctgg ccgaagcgtt caactcggtc ggcaccacga
    46441 tcggcccgct ggcgggaggt tatctcatct tcgattattt cgcccggagc ggagcgcatg
    46501 gggcggaatc ggtcaagcgg ccctaccttt ggtttggcct gacgctgctg ctcctggcgg
    46561 ccgtgttttg gcgcctgcgc ctgccgcgcg tgggcggggg ccggatcgag cgcggcgtgg
    46621 gcgcgctcaa atatccgcat gtcgtgctgg gcggggtggc gctcttcctg tacgtgggcg
    46681 gcgaggtctc ggtcggaagc ctgatcatca acttcctcgg ccagcccggc attgccgggc
    46741 tctccgaact ggaagccagc tcctacgtgt cgctgtattg gggcggcctg ctcattggcc
    46801 ggttcatggg cgccgtggcg ctgagcgcgc tgccgccccg ccgccggcag ttctggctgg
    46861 cggccttccc gctggcgggt ttcctggttc tatgggggct gcgcggcggg gaggccgtgt
    46921 gggggtattt gccattttta gccctgtgct ggctgctgtt tcagttcagc cgggcgcgcg
    46981 cgggccggac gctgctgctc ttctcggcca tggccgccgc gctgctgttc gtcgccctcc
    47041 tggctggcgg gcgcctggcc ctgtggtgcg tggtgggcgt cgggctgttt gcctccatcg
    47101 gctggcccaa caccttcgcg ctcgccctgg aaggcaccgg cctttaccag ggccaggcct
    47161 cggcctggct ggtcatgggc attctcggcg gcggcgtcct gccacccctc ctgggcggca
    47221 tggccgacct cacccacagc ctgcagttcg ccctgatcgt gcctttgctg gcgtttgccg
    47281 gcgtcgccct ctatggctgg aaaggacatc gcgtcggacg cgcccgcccc aactccggcc
    47341 gcgactgcaa ataagccttt gtcccccggc ggcggagggg ttaacttttg cctgactgat
    47401 gcagaaccgc ccgaaccatt ccgagggggc gccgaacccc ggtccgcccg gccgcctgcc
    47461 cgagctgctc gccccggcgg gagattggga gtgcgcccgg gccgcggtcg agaacggggc
    47521 ggatgccatt tatttcgggc tggagaagtt caacgcccgg atgcgggcca acaatttcac
    47581 cgaagccgat ttgccccagc tcatggagtt tctgcaccgg cggggcgtgc ggggctatct
    47641 gacgttcaac atcctggtct tcgaggacga gctggccgcg gcggagcaat acgtgcgggc
    47701 catgatctcc gccggggtgg atgccgcggt ggtgcaggat gtgggcatgg tccgcctgat
    47761 ccgccagcac tcgcccgact tccccatcca cgtctccacc caaatgacca ttaccagcgc
    47821 cgccggattg gagttcgcgc gcgcgctggg cgccaaccgg gtcgtgcttg cgcgcgagtg
    47881 ctcgctgccg gaaatcgcgg ccctgcggac gccgccggcg gggctgccgc cgctgccact
    47941 ggaggttttc atccacggcg cgctgtgtgt ggcctactcg ggccaatgcc tgacgagcga
    48001 ggccctgggc gggcgctcgg ccaaccgcgg cgaatgcgcg caggcctgcc gcctgccgta
    48061 tgaactggtg gccgacggcc agcgggtcgc gctgggcgac cggaaatacc tgctcagccc
    48121 gcaagacctg gccgggctgg aagtgctgcc cgaactggtg cggctcggcg tggcctcgct
    48181 taagatcgag ggccgcctca aatcacctga atacgtggcc aacatcaccc gcatctatcg
    48241 ccgcgccctg gaccagcgag gcggcgacgg acggatcacc cgcgccgatc gctatggcat
    48301 ggagatggcc ttctcccgcg gcctgcatac cggctggttc cggggaaccc accaccagca
    48361 actcgtgcat ggccgcttcg gcaaaaagcg cggcgtcttc ctgggcccgg tggcgcgccg
    48421 ggaaggcgag cgcgtctggg tcgaactcca ggccccgctc aagcccggcg atggcgtggt
    48481 ctttgatgcc ggctgccccg agcaggacga gcagggcggc cgggtttatg acgtgcgccg
    48541 cgcgggtcgc gggccggaga atggccggct ggcggaactg cgctttggcc ggggcgacct
    48601 tgatttccgg cgcatccatg ccggggataa actatggaaa accagcgacc cggagctgga
    48661 ccgggcgctg cggcagagct atgccggcga tgccccgcgg ttccagcgcc ccctccggat
    48721 ggaagcccgc ggcgccgccg gccaaccgct caccctcatc gtccaggacg aactggggca
    48781 tgaagtccgc ctggcctcgg cgatgccgct ggcgcgggcg caaagccagc cgctgaccac
    48841 cccccggctg caggcccaac tcggccgcct gggcggaacg gcattccgcc tggacgcgct
    48901 gcagaattca ctggccggcg aagtggtgct gcccgtcagc gagctcaacc gcctgcgccg
    48961 cgaggctgtg cggcaactgg acgcccggcg cgcccggccc cggcagtgga ccctgcggaa
    49021 cgacgcgccg gacgccgcgc gcgccaacgc cgcccccccg cccgccccgc ccggcgcgcc
    49081 gccgcatctg atcgtcctgg tgcgcagcca ggcgcaactg gatgccgcgc tgcacgccgg
    49141 ggtggagacg gtgtattgtg aatgggaaga cctgaaaaag tatcgggagg ccgtcgcggc
    49201 ggcgcgccgc catgcggccg ccacgggccg gcgggtggaa atcttcgccg ccccgccgcg
    49261 gattttcaaa gcgggagaag aatggacgct gcggcaggtc cgcgccagcg aagccgacgg
    49321 ctacctggtc cgcaattacg accatttgcg cttcttcgcg cgcgaccggt gcgtcggcga
    49381 ctactcgctc aacatcgcca accggctggc cgccgactac ttccagcgcc agttcggcct
    49441 ggagcggatc accgcctcgt atgatctgaa cgccgcgcaa ctggccgccc tcctgcgcgg
    49501 cgcgccgccg gagtggttcg agataaccat tcaccagcac atgcccatgt tccacatgga
    49561 gcactgcgtc ttttgcgcct tcctcacctc cagcgccgat taccgaacct gcggccgccc
    49621 ctgcgatacg cgggaagtca aactgcggga ccgcgtcggc gcggagcacc cggtcaaggc
    49681 cgacgccggc tgccgcaaca ccgtcttcaa cgcccgcgcc cagacaggcg ccgagtatgt
    49741 cgcgcgcctg caggcgctgg gcgcgcgcca cttccgcctc gaattcctgg acgaatcccc
    49801 ggcccaggcc agccgcgcca tcgcgctgta tcgccaactg cttcgcggcg agatttccgc
    49861 cgcgcaaatc agccgcgagt tgaagctgct caaccagctc ggcgtcaccc gcagcccgcc
    49921 ccgcctctga cccgcccgcc gccgcacctt tccgccggaa gcgcccgccc cggcccggcg
    49981 gctcaaagcg gggtgcgggc cgcccaaaaa aagggctgtt ctttcttccc ggaggcggta
    50041 gtttggaaac gaagcttagt tatgaaaaga accgtgtgtg tgttcctcgc ctgcgcggcc
    50101 ggcgcggtcg ggccgttgct gggttccgat aagccggtgc cgggctcgga gaatgtggaa
    50161 acggtggtca gcgtggatcc cgccacagcg gaagaagaca tgcggcgcga gcaggcgatt
    50221 gccgagttta ccgcccggat gaaagcggcg aactacccgg ctttgttcga ggccgcggca
    50281 aaggaattca acgtgccggc ggacattctc aaagccgtgg cctttgccga gacgcgctgg
    50341 gagcacctga cctggccgcc cggcgaaacc gaatcgccga aaaacggcat gccgcggccc
    50401 tacggcatca tgtcgctttg ggacaacaag tatttcgggc acagcctgct ggaagcggcg
    50461 gcgctgattg gcaagacgcc cgaggaattg aagaacgatc cctaccagaa tatccgcggc
    50521 ggcgcggccc tgctgcgcca gcactacgac gccaatccga agccggaggg aacgaccgag
    50581 gcggacatcg aaagctggcg ctatgccatc cgcaagtatt gcggcatccc ggagccagac
    50641 ctcaacgccc ggcacgcgct ggatgtttac gtgtttatga gccagggcta tcaccaatac
    50701 ggcatcgagt gggatggccg cccggtcaac ctggacccca tccgcgagga aacccgccgc
    50761 atcgtcgccg aggaagaggc gaaacaagcc gccgcgcagg cggacgaagc gcccgccgaa
    50821 gccccgccca ggccgaaact gtcctcccat ccccagccca aaccggaagc ggtgatggac
    50881 gaggaaatca cgccgccgcc ccccgcccaa aaatccatca agccgtggtg gattatcctg
    50941 gcggtcgtgg tggtcgtggc ggccagcttt ctgctgaagc gccggctcac ccgttccgat
    51001 tccgcctgac cccgttcccc ggagcgcggg cgccccgggc gttccgacgg ccggccgtgg
    51061 gggccgggcg ttgcggcgac cggcgatccc gcccgaatcc tgatgaaccc agcgatgaaa
    51121 gcagttgttc tgcgcggcaa ggaagaaatg caggtggagc acgtcccctc accgccgctg
    51181 caagcgggcg aggtgcgggt gcagatcgaa gccgcgctga cctgcggcac cgacctgaaa
    51241 gtctttaagc gcggctacca tgccaaaatg ctgatcccgc ccacccgctt cgggcacgag
    51301 ttttcgggcg ccattgcgga agtggcgccg gaggtggcgg gctggcgcgt gggagaccgg
    51361 gtggcggtgg ccaattccgc cccctgcggc cagtgtttct attgccacca gcggcaggaa
    51421 aacctgtgcg acgacctgct gttcctgaac ggcgcctacg cccgctccat cgtcgtgccg
    51481 gcgcggatcg tgcagaaaaa cctgctgcgc ctcaagccgg agaccgactt cgccgacgcg
    51541 gcgctggtcg agccgctggc gtgcgtggtg ttgggggtcg aagacgcgca gttgcgcgcg
    51601 ggccagcggg tgctggtcat cggcgccggg cccatcggcc tgatgtttgt cgcgctggcc
    51661 aaacacctgg gctgccaggt gagcgtcgcc ggccggggaa aaaaacgcct ggaaaccgcg
    51721 cagcgcctcg gcgcggacct gctcccgagc atcgaaggcc cggccggcct ggaagccgtc
    51781 ctggcttccg gaacgaggtt cgacgccgtc atcgaggccg tcggcaagcc cgagacctgg
    51841 gccgccgccg tgcggctggt gcgcaagggc ggcacggtga actttttcgg cggctgcccg
    51901 gccggcacca ccctgacgct cgacacctcg ctcatccact acgccaacct cacgctgcgg
    51961 gccagcttcc atcacacgcc gcgcacggtg cggcgcgccc tggagttcat cgaggccggc
    52021 gtggtgcgcg cggatgattt tgtggacggc gaatgcgcgc tcagcgaact gcccctgctg
    52081 tttcgctcca tggccgccgg caaccgggcg gtgaagacgc tggtgcgcgt gcgcgaataa
    52141 gccgggcccg cgccggaaag cgcgcgctcc ccgcggcggc gccggcgcgc ggctaaggcg
    52201 gcaccacctg ctgaaactcc ggggacgagc gcagcgcgcg gaaacgctca tccttccggg
    52261 cggtatcgag caaatcccgc gccttggaat cctgctggag ccgtttctgg ctcaggtcaa
    52321 ccgcctggcg aagggcgggc agggcctcgg cggtcttgcc gaggccgcat ttcaacgcgg
    52381 ccaggtcgta ccaggcctcc gggttggtgg gcatgagttt gaccagcgca tccagcgact
    52441 gctcgagctt gggcacattc tgcagcaccg catacgcctg ggccaggctg agcaccgccg
    52501 cggcatccag ttgcggctgc ttttccagct tgtccacggc ccgctgcaaa tccgcgtaat
    52561 gccccaggct gctgtacacc gggatcaggc tgcggaaaac gtcggcgccg gcctggggat
    52621 ggttcaggat gccttccagg atctgcaccg tgcgattggt ttgctggagt tggaggtagg
    52681 cgctcgccag gtcgagggcg gcctgaaagt tggagggatt ctgccgcacc tgttcctcca
    52741 gttgctggaa ggtgatctgg gcctgctcat agcccgcgct ctgctggtag ccccggatgg
    52801 tgtccaccag ccccttcacc tggccattgt agggatccag cttcaggcag gtctcggcca
    52861 ccaggcgcgc gtcgtcaaag cgccgcgcct ggagcagcag ttgcgcgtag cggaagacag
    52921 cctccgggct gtacgggcaa aaggcgatcg cctggcggaa ggcaaaatcc gcttccttga
    52981 atatccgctg gtactcctcg tgcgatttgg gccggtactc cggcgggcag atattgggat
    53041 cggggttcag gcgccaggcg taaatgccgc cgatggagct gcggagcttg gaaaaggatt
    53101 tctgggcatc gtcgtcgtgg acgaatttgc ggtcgccggt aaagccggtg aagtcgtggc
    53161 gcaggtaggt cttttcaatc caatcggtga tctgcttgac cggcgtgtcg taatccacaa
    53221 tgtccccggt cagccgccgg gcgaactgcc gccagaactg gtggtcgcgg ttgaggatgt
    53281 cctccgtcaa cgtcggcagc ggctggcggt tgatcttcat gatgatcccg aagggcgtca
    53341 ggtgcggata catccagtcc agcgggaagc tctcctccac gaagaactcg ttcttgggat
    53401 tgtgatcgaa gatgaccttg gtcagcaggc cgttgatgtt catgaccgcc acctggccgg
    53461 agacctgcac ccggttgtca accagcttca cgtcctcgcc cggttttaac tgccccgcct
    53521 gcaggcgccg ctgggcatcg gtcacatact cctcgaagca gcgccgggaa tcctccggcg
    53581 tggccgagta gatttcgcgg tcggggtaca cgccgccccg gctgcgggcg atctcgcggg
    53641 gatacgccgc ctccagcagc aagcggttga gccggatgcg cgtccagctt tgcgggttct
    53701 cctggatgaa gtccgtcagg tactccgaaa gctgaacctg ccggaagcgt tccggctcgt
    53761 aaaacggccg ggcctgctcc agttgggcca gctcggtctc gagtcgctcc tgcttccggc
    53821 gcagccgctt cgaagtgctg ccatccgcca gctcctggtc caccgcggcc ttctcctgct
    53881 gcaggtcgcg ggcgcgccgc ttgagcttaa gctcgcgctc cagcagcccg ttcaagtccc
    53941 gggccaggtt ccggcgcagg ctcgcctccg cgcccggggc gtccagcaga tgctgcgtcg
    54001 ccgggctgag gttctcatac agatacttcg agaccgggtc ctgctgcggg ccggggcgca
    54061 gccgggccgc gaacgcgggc aggtcaacaa agtcccggtc ggccagccac gacgtgtagg
    54121 tgcggcggcg cttttcgatc cggtcgccca gcgcggtgaa ggcccagtcc agcggcgcca
    54181 ccgcccgggc cagcaggttg gtctggtaat ccttgtcctt gagcaccatg cggaacagct
    54241 cgcggaagaa gggcggatca atctgggtgc tgcggttgta atgcgcgcgg atgtagcaca
    54301 ggtacgtccc gtcggccagg gcgttttgcg tgatgatata gacatcgcgc cggtcgaact
    54361 tctgatcctc tttgggctgg caggagtgcg gcgtgaagct ctcgcagaag atgatatacg
    54421 tcgggcaaaa gcgccccgga tccgtcccgc catacagcac cgcgttccgg gtcatctccg
    54481 ggtagagcgg ctggccggcc gcatcctgga agggcggggt gaacatatca tgcccgaacc
    54541 agtagccgaa ccagtgatga cgctggttgc tggggaacca gtgcgtaagc gcggaataaa
    54601 ccggcatggc ggcaaacagc cccagggtca ccaggagcgg cccgcgctgg cgataaacca
    54661 gcagcgccag cacaaagccc accgcgatgg ccagcagcag caggttggca tagatgggca
    54721 ggccatactg attcggggca aaggcgcgcc ccacccagtg cggcaattcc gaaagcggga
    54781 tcacccccgc cgggccaaag taatgcttgc cggtcgtgtt ccagaggcaa aacagcgcca
    54841 gcaccaccgc aaacatcccg cccgccagcc cccacagccg gaactgctga tagtgcgtcg
    54901 ccataaaggc cgccgtcagc gccagcccgt agccaatcag gcaggccaca atggtatgcg
    54961 aagagcagag aaagaccttg accaggtcgg ccgaagcccg gtcgggcgtg ggattgagca
    55021 gcatgatcaa cagcgccccc aggcacaggt aaagcgccga caaactgatg atccacgccc
    55081 gctcgcgccg ctgcatcttg aaaaagaaca ggaacggcac cagcccgata aacatataaa
    55141 cccaggtgaa ctcatcggcc acgccctcaa tcaacatccc caactggctg gcaaaccgcg
    55201 tcggatcggt gatcgggttg gtcgggttgg gctgctcata ctggccgcgg ctcagcgcgt
    55261 ggaagaaccc ctccaccgtg cgcggatacc cccactgcat gggcggattg gacatgcccg
    55321 acaccgccat gtagaggtag aacgacacgc ccagcatcca cagcccggcc atcgccagca
    55381 cggtcttcca gtacgtcccc agccccctgg tccgaaccgc cagccagatg gcggccacca
    55441 gcgagccaat cccgatggca ttgaacagga acagcaaccc gggcttggcc ccgatattgt
    55501 gaaacatgtg gtcgccggta aacaccacca gcaggcaatc caccaggtac acgatgaaat
    55561 tgcccagaaa cgcgtcgcgc cccagccgcg gattgccggc ggcaatcgcc acctcgatgc
    55621 ccagcgcggc cacaatgaga ctctggtgca gcgtaatgca gaccccgaac aggaacgccg
    55681 cccagtaggc gtagcgcatc tggtgcggcg cgtaaatcca ccgcagcaaa caaaccagca
    55741 ccgccagata ccacggcacg ctggtcacgg cgatgcgatt caccaccacc gactcgttcc
    55801 acatgaaccc gtccagcccc agcagcaacc cggcgcacac cgccgcgacc aggcaaatgg
    55861 cgctctccca tttgccggtc atgttcttca gctcttcaat cccctccatg agcatgctgc
    55921 tgccgcggga aaccagcagc gcgagcagac cgcacgccat ggcgcccgaa aatgcctgcc
    55981 ccaccgaaac ccgccaggcc atgttcccga acggcagcag caccgtccaa agccagctat
    56041 aaatcgtcca aatcgggtaa ccaggcggat gtggaatccc ggcataaaag gcccccgtca
    56101 ccagctcgcc cgaatcctcc agcgtcaact cgggcgccag cgtcagcaaa taaaccaccc
    56161 aaaccaccac aaacgtaatg gcgagcgaca gccagtcaat cggccggaac agtggcggca
    56221 cgggtccggg agcaggaaca ggcaacggct tcggcgcctt acgcccctcc gattccgtct
    56281 tctttggttt gatcataatg agtctctcga cttacccgga caccataacc aacaaaacca
    56341 gggcaacgcc agttgaatct tcaagccacc acccggaaca ccgcccgctc gaccaatcgc
    56401 cggaaaccgc cggggaaaaa ggcaaaagaa aaaggacccc cgccagcgcc gcaagccgaa
    56461 cggcaggccg gcgccgccgc caaagggtca tgcccggagg ccaacccgga ccggcagccg
    56521 gcatcctccg ccccaccttc cccgccctcc ccggccccga cccgaacgcc ggaccgctaa
    56581 agctcctcca agccgttcca cggcgccacg accgggcagc catccctccc tgcccacccc
    56641 ggcagcgatc ccccaacccc atacccgctt gcccgccatt cccactcatt tccccctgcc
    56701 aacaggccgt gtacaagggg tgtacaaggg atgcccaccg gatgtaccag ctaaaatccc
    56761 atgatctccc ggtgagcatc ccttgtacac cccttgtaca cggcctgttt ggggctatgc
    56821 agtggcataa tgaaagggaa ttggctgggt gggaggcagg cttggtggga gggatgggag
    56881 gaatgccaga acgggggtta ggggaagtgt ccaataatta gacacttcaa gttctcgcta
    56941 gagtgtctaa taattggaca gttttttggg ttgccgggga ggcaggaggg tggccggtat
    57001 tggcttcggg cggggtgtat gccggcgggg cggctcaggg gggaaggtct ttgccggtgg
    57061 aggtgatggt gagtttgccg tgtttgacgc cgcgcgcggc gatcagttca tcggcgatgg
    57121 ctttgacggt ttgggctttg ccgcgcacgg cgaggacttc caggcagtta tggtggtcca
    57181 gatggacgtg cagcgtggcg aggatgaggt ggtggtggtc gtgctggata tcggtgaggg
    57241 tggtctggac gtgctgtttg tggtggtcat agaccagggt gatggagccg gcgatttcgc
    57301 ggtcgccgta gttctgccgg tgctcgacca ggccggcgcg gatgaggtcg gcgacggcca
    57361 gggagcggtt ggcgtagcct ttttcgcggg tcatccggtc cagctgggtg gccaggtcgg
    57421 gagggacgga gacggtaaag cggacgacgt ggggtttttt ggcgggtttc acaggtttcg
    57481 cgggttgggg gaggcgccgg gatggaggcg gagattcggg cgggaaaggg aatcaggggc
    57541 ttttgaattg catgccgtcc cggttggcga agcgcacgat tacgccgtcg tcgccggtgc
    57601 gttggtacca ggtgagctgg ctcatggggg cgtggctgct ttggctgcgg gggctgcggg
    57661 gccgggggcc caggctccag agcacccagc gcaccgggct ttcggcgggc tgggagtaga
    57721 acttgccgtg gtcgcttttg aggttgggga aattggtggg cacccagagg gcgtagttgc
    57781 cggcgggctc gccgttgagc agcagcggcc cgggcgcggc gtatttatag gtgtggccgg
    57841 gagagaacgg gtcttccagg ttggcttcgc gcccgtcctc gccgctgcgg ggcaggtagc
    57901 ggctttcgga gagttcgccc ggcaactggc accagtggtc gcgcaagttg ttgttgcaat
    57961 tctcgcgcac gggggggacc cggccgccgt ggtcgtcggc gtacatttgc aaggccaggc
    58021 cgatgccgta aagctcggca tggaccttgg ccaccagggc tttttcgcgg gcccgggcga
    58081 tggccgggag cagcaggctg gccagcagcg cgatgatggc gatgacgacc agcagctcga
    58141 tgagggtaaa gccgggcgcg ccccgcggcg gggcggcggg gcgcgcgggc tcacactcgc
    58201 gacggcggct catgggagca gcttaagccg tcagcgcacg cgttgcaatc ggaagaagcg
    58261 cgcgggcgcc gccgccgggt cgagggtcac cgtccagcgg tcgttggcca gcacggggga
    58321 attggtcacc ggcagccagg ggccgccttc ggcgagggcc ggctggctga acaactggta
    58381 gttggccagc gaggccggcc aggaaaggac ggtgggggcg gggccggaca cgctgaggag
    58441 cggcggttcc gacggcagga tttgctggac gaacagctcg tagccgttgg tgccctgcga
    58501 gccggagttg ctttcctggt tgaggatgcc gatggcgtcg aagacatagt ccggcgcggg
    58561 gccgaggtcg tagcgggggt ggcgcagccg gaacatccgg tttgcgccgt cggtgacctg
    58621 gcagaggcgg ttgccccagg tgttggtggg gttccagccg ttggtgctca acagggtcag
    58681 gccgttgatc cggacgcgca tgctctgcca gtgttcgccg cccgtggcgc gcgtggcgtc
    58741 gaagatgtcc tcggacgtgc cgggattgtt gtcgtcgggg cgcatgagcg agagcagcga
    58801 gatgacttcc ggcgcgggca ggccgtagtt ggaggagacc agcgagatgg tgaagtcaaa
    58861 gtccggctcg ttgcggtgca tttcattgat gttccgcttg cccccgtaga agaggctggc
    58921 ctgggccgtg acggaaacga ggtcgccctt gcggaaggcg tggccggtga gcgggtcgtg
    58981 gttgagccgc gcgatttcgg ccagccactc gctattgcta tagctgaagg aatcatcgcc
    59041 gggcgaacgc cgggtcgcgt agttctggcc catccagcag aaggtgccgc cgcggtcgcc
    59101 cggcgcggtg gcctgcacca cgatctgcca ttcgccgccc atggtgccga cgtaggccgg
    59161 ctggaaattg ggcgtcgcgt ccagcatttc gtccgggtcg gtgagcaaaa cgccctgcac
    59221 cgtgagcggg taggcgggcg tccaggcgct ctggccgacg gcgttgacgg cttgcaggtt
    59281 ggcgtgggtt tccgcgagcg cggacgcggc cggcagcagc gcggccaggg ccagtgcgcc
    59341 ccaccggccg gcgcgggccg gcgtccgggg cgtggaggtc gtttcgtttg gtttggttgc
    59401 tagtattacg ttcatgccaa ataatatgaa gcaaggcgcg gcggagcgca aggcgaaaaa
    59461 gccgcccgcc gaaggcccgg ccccggcggc gggttccgcc gggcggcggc gcgggcggcg
    59521 gcggcggggg cgggcggcgg ggcgggaata agggtttgac accgggcggg cgcggcccta
    59581 ctgtggcggc acatcaattt tatggctgag aatattccaa atgttccgac cggcgcgatg
    59641 ccgccgaaga aggaaaccgg caaggttcaa cccaagaagg aaaccgtccg catcaatttg
    59701 ccgcccaagc cctcagccgc cccgacgatc aagctgccga cgctgccccc cggggggccc
    59761 gccggcggcc cggcagccgc ggctccggtc gcggcgcccg gcgcggcgcc agcggcccgg
    59821 ccggccgggg ctccgcgcgc cgccgcggcg ccggcggccc ccgccgcccc cgccgcggga
    59881 gcgggccgcg ccactcccgc cgccccgcgc atggcggccg cgcccgtggc cgcccggccc
    59941 gccctgggca ccctggacac ggtgctggcg attgcggccg cggtcattgg cgtgctggcg
    60001 ctcctgacca cgttgaacaa catgttcgag tggatctgga agacgtaggc cggcgggctt
    60061 ttctggtttt ggccgtgtaa tcaaccgcaa ccctgtttta tttgtatttg actatggcag
    60121 ctcccaatat cgtgaatgtg accaaggcaa ccttcgccgc ggaagtgctg aacgccgcgg
    60181 ggcccgtgct ggtggatttc tgggcggaat ggtgcggccc gtgcaaggcg atcgggccga
    60241 tcctggatga attggccgcg gaatacagcg gccgcgtgaa gattgccaag gtgaacattg
    60301 atcacgagca ggaactggcg gccgagtatg gcgtccgcgc catccccacg ctgctgctgt
    60361 tccagaaggg ccaggtcgcg gagcagatgg tgggcctgcg cagcaagcgc gagctccaga
    60421 acagcctgga gcgcgtcgcg gcctgagcgg cttcggaagc cgccgccgga agcgcgcggc
    60481 tccggaaggg gccggccgcg ccgggaccgc cgccatgaag gcgtttatct tcacgcccgg
    60541 gcagttagcg cggcgcgcgg atttctatta ccagctcggc caactgaccg gcgccgggct
    60601 ggggctggtg caggcgctgg agcaggtccg gcgcaatccc ccggcgaatt cctaccgcgc
    60661 gcccctcggg cgggtgctgg tgcagctcgc cgcgggccgc acgttcagcg aggcgttgtg
    60721 gcagggcggc ccgtggctgc cggagtttga cctggccctg ctgcgggccg gcgaacacag
    60781 cgggcggctg gaggcctgct tccggttgct ggcggattat tataccgacc gcgcccaact
    60841 ggcgcgtcaa atgatcagca acctggttta cccggcgttt ctgctgcact tcgcggtttt
    60901 tattttcccg ctggccacgt tgctgctctc cggcgactgg gttgcctacc tgggccagac
    60961 gctggggatg ctggccccgc tttacgcggg ggtggggctg gggatcttcg cggcccaaag
    61021 ccagcatggc gaatggtggc gggcggtttg ggaggcgctg ctgcatccgc ttcccgtgct
    61081 ggggccgggg cggcgctgcc tggcgctggc gcggctggcg gcggcgctcg aggccctgct
    61141 cagcgccggg gtgacaatca tcgaggcgtg ggaactggcg gcggcggcca gcggctcgcc
    61201 ggcgctgcgc cgggcggtgc gggcctggca gccgcaggtc aaaggcggcc agacgcccgc
    61261 cgaggcggtg cgcgcgtccg gccgctttcc ggagcttttc gccacgcagt acgccacggg
    61321 cgaaatcagc gggcggctcg acgactccct gcggcggctg cgggactact accaggaggc
    61381 gggcgcgcgc aaattgcgcg cggtgacgcg ctgggtgccg cgcgcgattt atatcgggat
    61441 tatgctgctg attgccgccc gcgtgcttct gttctacacc cggtacttca acctggtgcg
    61501 ggacgcgggc ggtttctagc ccgccgggcg ggcgcgggac ggcgggtcgg cggacgcggt
    61561 tgccgaagtc ggcgcggcgg gcgggcgggc ggattttggg aaccgccggg agcggcgggc
    61621 ttttgccttt ccattccatc cggcgggcgg ttaaatgacc cgatgcgcga atcatcactt
    61681 gctttcctga agacgctggt caacacgccc agcccaactg gttatgaaac ccgcggccag
    61741 cgggtctggc tggattacgt ggcgccgttt gccgaggaga ctttttcgga cgcgtacggc
    61801 aactgcgtcg ccgtgttgaa ccggggggga tcgccgcgcc tgatgctcgc cgcccatgcc
    61861 gatgaaattg ccatggccgt gagctacatc aacgaccagg ggtttattta cgtgcgcaag
    61921 ctgggcgggg tggacgcggc catcaccaag gcccagcgcg tgatcattca cagccggggc
    61981 ggcccggtca agggcgtcgt cggcaacctg gcgccgcacc tgatgaagga ggagaaagac
    62041 ccgaaaccgc cgcggatgca cgatttgttc attgacatcg gcgtggccag ccggaaggag
    62101 gcggcgaagc tggtgcgcat tggcgacccg atcaccctgg acgatgagtt tgagctgctg
    62161 cgcaatgacc tggccgtggc gcgggcgttc gacaaccggg tgggcacttt cgccgtggcg
    62221 gaaacgctgc ggctgctgcg ggagtcgccg gggcggctcc gggccgaggt gtgcgcggtg
    62281 tccaacgtgc aggaggaagt cggcctgctg ggcgcgcgcc agatcgccta ctcgcttcag
    62341 cccgatttgg cgctggtggt ggatgtcacc cacgccaccg attaccccgc catcagcctc
    62401 ccgcagcacg gcgacatccg ggtcggccgg gggccggcgc tcacccacgg cggctgcaat
    62461 cacccggcgg tggtggcgcg gctcgaggcg gtcgcccgcg cccagcggat cccgctccag
    62521 cacgaggcca tgtccgcctc cagcggcacc gacaccgacg tgattttctg gacgcgcggg
    62581 ggcattgcca gcgcgctggt cagcctgccc aatcgctaca tgcactcgcc ggtggaagtg
    62641 gtcagcctcc gggacctgga gctgattccg cggctgctgg ccgcctttgc ccgctcgctg
    62701 gcgaagggcg agcggttcaa ggtcaaaatc tgagcgggct cagtgcgggc ggtgcatctg
    62761 ccagatgatg ctgccgccgt gaatcagcgc caggatcagc aggcccgcgc ctgaccaatg
    62821 ctccatgcgc agcagcgtcc gctcgctcag tttgccgtgc ccgagcgagg ccgcccagct
    62881 gaggcccgca aaccacgcgc tggtggccac ggccaccccg gccacgcagg ccagcttgcc
    62941 cggccagtcg ggcgtgaccc actcgcgcga gatgaagttg gccgccagga tgatccagaa
    63001 gaccagcacc ccgacgttgc ccagcacccg caccgcgccc gtcatgaagg ccgagtgggg
    63061 atgcagccgc tcctccagcc gcgcttcgag gcgctccgcc gcgccgcgca gccgcatcgg
    63121 cggctcatgc accgatttgg agagcaggaa cttgaacccc agaaacagca ggaagacgaa
    63181 gctgaacagc tccatggccg ccttgacata gccgcgcgtg aagaaggtcg caaacccggt
    63241 gaacgcgata aaacagtaga tcacctccat ggtcgtcgcc cccagcccga tcagggccgc
    63301 ccatttgaac ccgcgccgcg ccccttcgtt caggatcgtc aggttgatcg gcccgatggg
    63361 gatcgagagc agcagcccgc tgatgatccc ggtcagcccg gcgactaaaa tcggcggcag
    63421 cgagtgcatg cggggtcagc ccggcgccgc cggccccggg agcgcctcct cgtcctcctc
    63481 ctcgtcttca atttcgcgcc gcatccggcg cgagatgtgc ggccggataa acccatagac
    63541 caggtacgcg gtgaaaaaca ccggcaggac aaagaccagg agcttctcgc ggaagatcat
    63601 gatgctgccc aggaacagga tggccacgag cgtcttggtg aacgtccgcg tggcccgcag
    63661 gtccagcgtc ttgaagctcg ggtatttcac gttgctgacc atcatccagg acaggaacag
    63721 cagcagcacg ggcaggcaat agcgccacca gcccgtggcg aattcctttt catcccacca
    63781 gatcatgaac agcgtcagcg aggccaccaa ccccgccgcc gacgggatgg ggaagccgag
    63841 aaactcgcgg gacgccgccg ttttcatggc cgccaggcag ttgaaccggg ccagccggaa
    63901 ggcgccgcag agcaggtaaa tggaggcgat gaaccagccc cactccgggt gcccgtggaa
    63961 gacatccttg agcacgaccc ggtgcaccag gaaagccggc gcggccccaa acgagatcag
    64021 gtcagccagc gagtcgaatt cccgcccgaa cgggctttcc accccgccca tccgcgccac
    64081 gcggccgtcg aacaggtcga agatgcacgc cagcagaata aaccccagcg ccaccttcac
    64141 cggctgaaag tcgcccccgg aaatattggc ctccacgatc ttcgtcagcg cgacaaagcc
    64201 gcagaacagg ttgcccgccg tcaggaggtt tggcagaaag tagattttga gcttcggctc
    64261 gtccgcgtcc gcgcgcggcg gggcgggcgc ggccggtttc tggggggggg cgctcatgcg
    64321 tgcttattcc caggcggcca gcaccgtctc gcccccgacc acatggtcgc ccagcttcac
    64381 ccggattttt gcgtcggccg gcaaatagac atcggcccgg gagccaaact ggaccaggct
    64441 gatgcgttcg cccggggcca ccgtgtcgcc caaccgcgca aaaggcacga tccggcgcgc
    64501 gatcaccccg gcaatcaccc gcaccgccac ccgccgcccg ggcggttcgt ccgccgtgaa
    64561 ccccagcagc acattttcgt tgtgccgggc cgagtcggcc cgcatggcgt tgagaaaccg
    64621 gcctttcgtg tattttagcc aggccacctt tcccgcgatc ggcgcattct gcacatgcac
    64681 atccagcacc gagaggaaga tggaaatccg ctggcattgg ccgcccatga actccggctc
    64741 gtttcccgcc tcgagggcat ccaccgttcc gtgcgccgcc gccagcacca accccgcgcc
    64801 cgccggcacc cgcggcgtcg ggtcgcggaa aaagaagagg gtgaagagcg caaacaccag
    64861 ccataccccc gccaccgccg ggataatcag ccgccccgcg cccgggacca ggaagcccag
    64921 cgccccgatc gccgccagca ccggcagcga aagcgcaatc agcttgaggc cggccttcct
    64981 cgctttgccc gaatgtttca tccgccgcca gcctaaatcc gcccccgccg cgcgcaaagc
    65041 ctaaagttgc cgcccgggaa ccgggggccg gtcgggcgct ccgcttttgc cgcggagaat
    65101 gaaatcatgc ccccgcagca ccgcgccggg cccgtatttc ccgcgcagcg cgtccacggc
    65161 gtcggccagg cgctgccgcg cctcgtgctg ccggacggac gcgtccaggg caagctgcgc
    65221 ccggaaacgc ccgtcgtaga ggttcgagag cttgagcgcg accagccgca ggcgcacccg
    65281 ccgtttccat gcctggcgca ggagccggcc gatctcgccg tagatttccg tctccaaatc
    65341 cgtgggctcc cgcaggctcg cgcccgcctg ctcctcgctc atgtcgttat agcggacttt
    65401 gacggtgagc gtgcggatgc tcttgccgtc cgcgcgcacc ctggccatga gccggtcgcc
    65461 catgcgccgc agggtggcct ccaggtattc ttcgtccgcg gtgtcggcgg cgaaggtttc
    65521 ctgctcgctg taggacttgg ccggcgcgcg cgccggcaca acgggccggt cgtccacccc
    65581 ccgcgcaaac tcgcgcaact gcggggccag gcggcccacc accatctcca gcaggcgggg
    65641 cggggtctgc gctatctggc caatgcgaac cagcccggcg gcgttgagcc ggtttgccgt
    65701 ctgcgggccg acccccggca gccactggtt ggagagcggc gccaggaacg cggcctcctc
    65761 cccggcgggc accccgagaa aggcggcggg cttgttgatc ttggaagcga tctggctgac
    65821 gagcttgttg gcgccgatgc cctcgctgac ggagagcttg agcgcctgcc ggatggcccg
    65881 gcggatcgct tcggcgatgc tcagcgccgg ctggcgcgcg cccgtcacgt cgaaatatcc
    65941 ctcgtcaatc gagccgattt ccacatccgg cgtgaagtca tacgcgtagg aaaacatcag
    66001 ccgggagaag agctcatact tgtcgaaatc ccccggcagc aaaatcagcc gcgggcacag
    66061 ccggcgcgcc aggagggtgg gcatcggcgt gtagatgccc agccggcgcg cctcatacga
    66121 ggccgaggca ataatgccgc gccgctcgcc gcccaccgcg atcggcttgc cgcgcagccg
    66181 cggatccgcc gcctgctcca ccgaggcgaa gaacgcgtcg gcgtccaaat gcacaatcgc
    66241 gcgcggccgg gccatggtcg gctccgttca ttggcgctgg cgaatcagcg cgaccatgac
    66301 gccctggatc agcagctcct cctgggggat gagattcggg tagcggggat tctccgcgcg
    66361 caaatacggc ttgccccgct gccgcaggaa cgtcttgagc gtcacctgcc cgtcaatcag
    66421 cgcggcgacg acatcgcccg gccgcggcgg cgccccgtgc tcgataatgg caatgtcgcc
    66481 gtccacgatg ctcttgccga tcatcgagtc cccccgcact ttcaggccaa acgtccgggc
    66541 cgtcggctgg atgccgagcg tcgtcacgtc aatcagcacg cagccttcct cctcttgcgc
    66601 cgcgtcctcg ggccggccgg cggggatgga gccgtaaatc ggcaccgaca ccacccgcgg
    66661 ccggggccgg ttcttcggcg cgttcggcgc caccacctgg agcgaccggg cgcgcccggg
    66721 ccgattccgc agaaatccct tggccatcag cgcctgcaca tgcgccagcg cggcattcgg
    66781 gctgttgaac cggaaatgcg cggcaatctc ccgcagggtg ggcgtaagcc cggcggaatc
    66841 ctggttctcc tggataaacc ccagaacctg ctgctgccgt tcagtaagct cgttcatact
    66901 gaacaaatgt ccagctacgg gcgcaatttg tcaacccgat ttgccgccgg ctccg
//
LOCUS       DAOPQG010000003        63538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_584_length_63538_cov_15.114503, whole genome shotgun sequence.
ACCESSION   DAOPQG010000003 DAOPQG010000000
VERSION     DAOPQG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 63538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63538
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_584_length_63538_cov_15.114503"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="PKI07_00540"
     CDS             <1..2066
                     /locus_tag="PKI07_00540"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49567.1"
                     /translation="KWEKHGLRGMPVIRHTASRFAGFKDYWNAYVPAALLPRLYGYEL
                     MMAPYTIAHMKLALKLSEINARLGQPDYQFRYEGRVHIYLTNALEPASGFATRQFEPF
                     FPALAHEASAVGDVKRRRRFTVIIGNPPYSKISSNLTPEMRATVERYRYLDGERIKER
                     GALQFEINLQDDYVKFFRFCEDRVTSSGCGVLGVITNNGYLSTPTLRGMRDSLLETFG
                     SAWIVDLHGHLAKGEVGPEGKAEENVFDIVQGVSLFLGVRTGAVRGDGTVQHGERYGS
                     RGEKYAFLEANRCSTTKFTRLEPSAPFYLFVPHDADLEQEWKGYIGIGELFPRNSAGI
                     ITARDGLVVCEDRRELAERLERFSRARDEDDAIYAEFGFSESKRFDLREAQAELRRLR
                     SFSEPIRKMLHRPFDVRFVFFHPSVVWSLSRPMAEQMVDGKNLALVATRQVTRPQFEH
                     AFVSRHIIEIKACSHDRNTQIFPLFIRGSDGELALSSGWAANLDTGAVGRFSRALGVP
                     FNGAACDGRDGDLSPHSVFYYAYAVFHSPAYRTRYAEFLKIDFPRLPLTGNLELFRAL
                     ARLGGELVALHLLESPKLDSPITEYLGGRAPEVEKVTWAKNTVWLDKAQTVGFKGVRE
                     EVWNFHIGGYQVCEKWLKDRKGRVLSADDRTHYQKIVVALSETIKRMAEIDRVIEKHG
                     GWPMK"
     gene            3267..4076
                     /locus_tag="PKI07_00545"
     CDS             3267..4076
                     /locus_tag="PKI07_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC49568.1"
                     /translation="MIASRAKRYSRDSFQPRGFSLIELLTVISTIAILAALLFPILNK
                     TKVKAQRTTCLSNLRQLGFAWALYRDDNGDYLAESYPGDRDPNPDVWVKGDMTKLQDA
                     GNADLLREGKLYPYGGSAPIYHCPADRGVSIGGKIVPTVRSYSMNSFMGARPSSAPMI
                     PSTARGYVDFFARGSDIPRPEQMWVLLEEDSRSINDGFFVTDPKGQIWYDWPTISAAR
                     HNYSYALNFADGHSATWRVTDPRSLRVTITKTEQPGNADLQRLASATTVRK"
     gene            complement(4186..10071)
                     /locus_tag="PKI07_00550"
     CDS             complement(4186..10071)
                     /locus_tag="PKI07_00550"
                     /inference="COORDINATES: protein motif:HMM:NF020846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC49569.1"
                     /translation="MIARPKAAPESPPSANAGTAKRRPARGASMLAAACCALLLWPAG
                     MSARGAERQRLSGHVPGGARHWAALGRLPGTNRLELTIGLPLRNREALRDLLEKIYDP
                     FHPEYQHYLAPAEFVERFGPSRQDYEAVIAFARARGLRITATHPNRMLLNVSGAVADI
                     ERAFRVRMQSYRHPTEARNFYAPDAEPSLDLQTRVLMIDGLDNFSRPRPANLRWVPQG
                     RGAGADSGARPAGAGSGPRGFLLGRDFRAAYAPGVGLDGAGEAVGLLALDGFYTNDVL
                     AYQNLAGLPHVPVTSVLVDGFNGRPGAGNIEVALDIAMASSMAPGLSQIIVYAGRPTS
                     SPCTVLNRMANDTNHLGQVAARQLSSSWIWRTPSLELENQIFLQFAAQGQSFFQASGD
                     DGAYCGAASPPWAPTDNPNVTVVGGTQLTTQSPGGAWAGETCWSYFPDEPVASGGGFG
                     TNHALPGYQTGIDLAARGGSDTWRNSPDVACVAADIWLIANNGEQFSGAGTSAAAPLW
                     AGFAALANQQAAARGLPALGFINPALYRIGKSSEYAAAFHDTTTGNNTNNCCGPDRFP
                     AGPGYDLCTGWGTPTGSNLIAALLAPPPALRVTPAALPAFSGPLGGPFRPAAQGFTLT
                     NDSHAALAWSVTHAAPWLHVSPASGILTHGGPAASVTLTPTAAADSLPVGSYVATLWF
                     TNVNTTNAADEFSQSRLVSLDVVAPPVIAAQPTNQTVFEDMTAVFTVVPANGASCAYQ
                     WQYDNGVYLTNLTDGGRVSGSASSSLVIAHAAPADAGAYSVIVSNAAGAVASVPAFLA
                     VFPWRPVIMTPPAGRSVVAGETVTFSVEAVGGQPLFYSWQRNGTALSDGRQVSGSHTP
                     HLTLHCVSPADAAAYSVVVTNADGMAASPGAVLTVASITPPQTALTTIYSFTGGSDGA
                     NPNALRQGANGSFYGTAQNGGAHGAGLVFQLSPAGLVQPLYSFTGGDDGATPFGALAP
                     GPEGDFYGSTYQGGAHDNGTLFRLTPGGGLTTLVAFNITNGSLPCAGLAPGADGNFYG
                     STYQGGAHGRGTAFKVTPAGALTTLRAFSNGADGGHLAAGLVRARDHTFYGATYKGGA
                     SGHGTVFQITADGGFKSLASFGQTNGANPLAELIQDPAGNFYGTTSAGGAHRHGTVFR
                     LSPDGALHPLHSFSGGADGGYPAAALLLGGDGNFYGTTAYGGDYGAGTVFRLAPDGTL
                     TTLVAFNGYAGANPQAALAEDDDGLLYGTTQNGGEYGLGTLFHLGFSGPLQITDPPAS
                     QLAYQGETVVLSVAVSGARPFSYQWRKDGASLPGATDRVLILAGVTTNDSGSYTVQAA
                     NPAGATNSSAALLQVLSAPPIIVHAPAPVTPAACSTVTFAATAAGDLPLAYQWQKDGA
                     SLADTCHRFGATTPSLVISNVTEADNGLYTLVVSNSLGVASASAWLTLVPASAPGTSL
                     TTRHWFGGGLDGGEPDGLAPAGPDLFYGTTRAGGDWNQGTVFACDIHGACVRLASFAG
                     TNGSSPRAAPVPGADGRYYGTTFQGGAHDQGTVFALTPAGALHTLHAFTAADDGAHPA
                     AGLLAGDDGSFYGTASAGGAFGYGSVFRITTNGAFTTLHAFTGGLDGKSPAGALVRGA
                     DGGLYGLTPAGGAFDQGCFFRITAAGELTPLYSFTGGRDGSSPVGTLALGSDGNFYGC
                     TETGGLGWSGTVFQITPAGALTTLHPFGDLLLKDGVRPAAGVMQSCDGNFYGTTRTDY
                     LGGYGTVFRVSPNGGVFTTLLYFDGCNDGALPRAALIEDAAGHLYGTTACGGPCYAGQ
                     GTLFRLSFDGAPAITTQPACQAVVPGACAQFSVAVTGARPLVYQWQRNGTNLWDDANI
                     SGSAQRNLILHSASPADAGLYRVTLTNAFGAVTSAVARLTVVPPPVFLSAARSNSTLA
                     LTWSAIPGQQYRLQQRPGLEAAAWTNLGKIVVPTSNTVTLTDNVGSLSRRFYRVVLLP
                     QGP"
     gene            complement(10231..11349)
                     /locus_tag="PKI07_00555"
     CDS             complement(10231..11349)
                     /locus_tag="PKI07_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOC49570.1"
                     /translation="MPEPPDFWEALFGPATGAPAGAWMNLRLVRKHGRPFLLLPRQRR
                     AAALSLSLYPAQTPRARAARALLRWAVGGAIPLGTRPLSFAIAPEDDFVRFIASLAGE
                     PKPALPTMGILAGNPAKDDQRFLILVFDAGQNPVAVVKAGLSERAQALVEKERALLAA
                     MPPNLGSVPRLRAHFQSPRQRALAMDFAPGDSPRPRDEGALPALLTSWVDTQRTLALG
                     ETADWQRLTRAVQARELPNPLLQRLPGRTVPATLQHGDFAPWNIKVSPASGAWTVLDW
                     ERGELAGIPGWDWFHYVLQPAILVERLPVAGLVQRMEGLLRSEAFLAYAERAGIRGCE
                     RELALAYLIHAAEVIKPAEGLAATNELRRALAARWQWV"
     gene            complement(11354..12736)
                     /locus_tag="PKI07_00560"
     CDS             complement(11354..12736)
                     /locus_tag="PKI07_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49571.1"
                     /translation="MSAEAQEISQLVRAVFRAWDQDGMAFLILRNYEGLPDFTTNDID
                     VLVAPGQRRRAEQTLVAAAREAGFRLHNRAEFMPVALYLSSRRSHAQAHFDLFDASKW
                     RAFDFLGCRGFLERRVRRELFAVPHPADEAATSLLSFLIYTGRVKEKYRAFITAQCRA
                     EAAAMTKLLAQTYGAARARFLVAAGAEQRWTAIESAAGALRRTLILRQLTRHPGRTLR
                     SVLADAKRLTLRFLRPPGLTVVLCGADGCGKSTIARVLMDELSCTFSPLKGRHFHWKP
                     PVFTARRRAARGPATNPHGQPVRNRFASLVYFLFHWLEFFLGSHLSFRPTTFRGGLVL
                     VDRYYYDFFVDPGRYRLQLPPGLARLGYAFLKKPDLVILLDAPAEVLQSRKQEVPRPE
                     TERQCRAYRALVSRLPNGRIIDAAQPIEKVGADIARVILDYMAERTQRRLGTRPGPPL
                     PETERAKPPL"
     gene            complement(12785..13030)
                     /locus_tag="PKI07_00565"
     CDS             complement(12785..13030)
                     /locus_tag="PKI07_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HOC49572.1"
                     /translation="MTPNYDKIDDAILALLHLTSFKENSGALKSTRAWKTHDWDALAR
                     LHAKRYISNPVGKAKSVMLSEEGKARAKELFCRLFCD"
     gene            13134..14387
                     /locus_tag="PKI07_00570"
     CDS             13134..14387
                     /locus_tag="PKI07_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOC49573.1"
                     /translation="MKPIQKASVCGLGKLGACIAATLAQRRFEVVGIDIDPEKVARIN
                     AGQPPVEEPLLAETITAGRARLRATQDYREAVATDVTFFIPPSPSLPDGSFSNEFLLR
                     AMQPIAAAVRAQGKSGHLFVCSSTTTPGAVDTVLIPMLERETGGVCGRDFGVCYNPEF
                     IALGNVVKGLLEPDLVLIGESDPQSGAVLEALYRQYNQNQPNIARMSIISAELTKISL
                     NSYVTMKISFTNQLRMIAEQFPKANIHQILDAIGSDSRVGRKYLRAGLSYGGPCFPRD
                     NRLLAYTARQLNLDAPLAEAADKVNHRANAALFERVCRLVSKTDTVAVLGLTYKPDTY
                     ITEEAAGLFLAQQLKRNGYKVVVHDYGARPEIEPSVIEFEHLADPATLAQRADIKLAV
                     ICCPWPQYRSVKFAPATQVLSPWKL"
     gene            complement(14408..15196)
                     /locus_tag="PKI07_00575"
     CDS             complement(14408..15196)
                     /locus_tag="PKI07_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOC49574.1"
                     /translation="MQSPENQVKAGRANVLGVGISAINLDTAVAAIAEALARKTKGYV
                     CVTGVHGVSEAQADPAFRTILNRAFLTTPDGMPTVWMGRWQGFRQMRRVYGPDLMLRV
                     CEFTRTRGYTHFLYGGAPGVADTLKARLEQRFPGLQIAGTYTPPFRPLNPAEEQQLQD
                     LVGKLRPDIFWVGLSTPKQEKFMAQYWQRLEATLFFGVGAAFDFHAGRMRQAPRWMQR
                     SGLEWLFRLGSDPGRLWKRYLKNNPLFILRALGQVTRLRRYSLD"
     gene            complement(15282..16841)
                     /locus_tag="PKI07_00580"
     CDS             complement(15282..16841)
                     /locus_tag="PKI07_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HOC49575.1"
                     /translation="MRGPGPLIALLAALVLALWSLDLGRRPLHNDEAVNGVKFGRLWS
                     GQGYRYDPNEHHGPSLYYATLAISRLTGAPPDADSFTERRLRFTPVLFGVGLLLLLPL
                     IADGLGRRAVVWAALWTVVSPAVVFYSRYYIHEVLLVFFTFLALAAGWRYWRRRRMGW
                     LLLAGAAVGAMHATKETFVIALAAAALALGLNQLWNRWLDAGAAPSARPGGIQLSHVA
                     AAAGAWLLVALLLFSSFFTNAAGLLDSVRTYLPWLARAGGASPHVHPWSFYLHRLLFF
                     QAAPGPVWSEALILALAVAGARAAFVRRGLGDANAGFVRFLAFYAFALMAAYGVISYK
                     TPWCLLGFWHGMILLAGVGAAWLAGRARRRAGRMVVTLLLLAGAGHLAWQAVRANGEY
                     AADRRNPYVYAQTSPDLLRLVRQVEALARAHPQGRQMPVKVMAAEDDYWPLPWYLRNL
                     KRVGWWDHLAPPPDAPVMIVSASFHAGLDERQTHVMAGYYALRPQVFLELYVALDLWR
                     AYLAQHPPEPD"
     gene            complement(17050..17763)
                     /locus_tag="PKI07_00585"
     CDS             complement(17050..17763)
                     /locus_tag="PKI07_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOC49576.1"
                     /translation="MNTTRTWLRRTMALSLLSGLAIAVLNLSVTPSQAADNANLEPLA
                     LKLPAPAFKGTPKDVDVGPNVEPLSDKPRPPMMVPRGLKNLAPGSKLTSSDKNATSDI
                     LARITDGDKDASDQSIIFLRKGTQWVQMDLGSPQEIFAVVIWHAHNMAKVYRDVIVQV
                     ADDADFIENVRTVFNNDADNSSGLGVGTQREYFETHEGKLIDAKGVKARYIRFYSKGS
                     TESALNEYTEVEVYGRPAK"
     gene            18176..19297
                     /gene="hisC"
                     /locus_tag="PKI07_00590"
     CDS             18176..19297
                     /gene="hisC"
                     /locus_tag="PKI07_00590"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOC49577.1"
                     /translation="MVTLPYPTNPNLQAVPVYQPGRPIEEVARELGLPADDIVKLASN
                     ENPLGPSRRAVAAMRKALAQAHLYPDGNAHYLKQKLAARLGVTPAHLILGNGSNEVIE
                     FLGHALIAPGTEVVMAQYSFAVYAIVTALFGGQLVVVPARNYGHDLDAMRAAITPRTR
                     LVFVANPNNPTGTCVAAADLARFVAAVPENVVIALDEAYFEFLDQPLDLTPEIRAGRK
                     PNLILMRTFSKIYGLAGARIGYGIAHPALIAELEKIRQPFNINAIAQAGALAALDDAA
                     HAQKTRRLNARGLRFYARAFRRLGLEYIPSAANFILVRVGDGQRVFHEMQQLGVITRP
                     MGGYQLPAWIRISIGTPRENKHCLAALQTALARTAAAPA"
     gene            19318..20598
                     /gene="argA"
                     /locus_tag="PKI07_00595"
     CDS             19318..20598
                     /gene="argA"
                     /locus_tag="PKI07_00595"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HOC49578.1"
                     /translation="MKLTDLRGILRYIPQFRDKTFIINVDGAIVTDENFTNLLVDIAV
                     LRSLNIHVVLVHGASAQIRALARETGRAPSNLDGTGITDEATLQLALTAANRLTHEIL
                     EGLAANDLRAACTNAVIAHPAGILHGVDQLFTGKVERVDTELLQRLLAQGIIPILPPL
                     GFDGEGKTYRVNSDGVALAAAEALKAAKLMFITAHDGIRVGERIIRQMPLADLESVLT
                     LQRQAIPPDLLSKAIHAAAACKAGVPRVHVINGSLPEGLLAEVFTNEGVGTLIYGNEF
                     QQIRRALKKDIRSILNLTRGSVESEELVKRTRPAIEKQLADYYIFEIDKNPVACVALH
                     TWPELGKGELACLYVSSAHENQGIGRKLVQFVENKARELGLQELIALSTQAFTWFQSK
                     GGFAEGTPDDLPPARREKYDQSGRHSKILLKKLK"
     gene            20683..21036
                     /locus_tag="PKI07_00600"
     CDS             20683..21036
                     /locus_tag="PKI07_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOC49579.1"
                     /translation="MRPYFPTLLLLICSNVFMTFAWYAHLKNLADKPWLLAALASWGI
                     ALFEYLLQVPANRIGHEVMSVAQLKILQEVVTLTVFVPFAIFYLKEKPGLDFLWAGLC
                     LLGAVFFMFRSKLMT"
     gene            complement(21106..22680)
                     /gene="dacB"
                     /locus_tag="PKI07_00605"
     CDS             complement(21106..22680)
                     /gene="dacB"
                     /locus_tag="PKI07_00605"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOC49580.1"
                     /translation="MNQLKCPVFTGFERRLAALICVFGAAALVFSAPAQPQKTAELKP
                     PATLAELRQRLTDHLAQPRFAAALWGVKIVALESGATVFEHNAGKLFTPASNSKLYTV
                     ALALDRLGGDYRIRTSLYAAARPDSSGTLAGDLVVYGRGDPGFNERLNGGDIYRALDP
                     LVNALTNAGVKRITGDVVADESYFRGPPFGAGWMWDDMEYYYGAEISALTINDNTLRL
                     RVQPGPAVGAPCRLSLQPATAWLTFSNRAVTVAKGAARSIHLYHPLCENLIYVSGQMP
                     VDGTNYTDEVTVHNPAGLFAGFFREALIRRGIAVAGRARSVNWLDREARPVDYGRQVE
                     LGAVASRPMRELAREVQKPSQNLYTDLLLAHVGEQSRTANTAARATSEDLGIRELNRF
                     LGQAGIPAGQTIFEEGSGLSRDNLTTPGATVTLLQFMHRHPESQAYLDALPIAGVDGT
                     LRNRMKGTPAAGNVRAKTGSLRWTSSISGHVTTAAGERLLFSIMVNRYAGATSVRADQ
                     DVILAMLAGFTGRATE"
     gene            complement(23316..24320)
                     /locus_tag="PKI07_00610"
     CDS             complement(23316..24320)
                     /locus_tag="PKI07_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOC49581.1"
                     /translation="MRKPRTTVYSWPAIERMLRIHQLIENKEYPNCHTLAREFEMSVR
                     TIKRDVDFMRTRLNLPLEFDPARNGYFFTRSVPHFPQMPISEADTFALFIASKAIAQY
                     RGTPLQQMLESTFRKLTGQLDQSARYSLGSLDGVLSFRPFAPGDAELAVFALLTQAVS
                     ERRAVRFSYRNRGQLKAQQRHVHPYHIACVNNQWCLFGFDVTRKAMRTFVLARLSKAK
                     FTGRRFTVSKKFDLNEYLQGSLGLFKGQDDFEVVVELDAFAADDVRGRRLHSSQELTE
                     MPGGMLRLRLRLDSLEEAERWVLGMGGHATVVRPEALRARLAAVARELTRKYGGGAQS
                     "
     gene            24630..25676
                     /locus_tag="PKI07_00615"
     CDS             24630..25676
                     /locus_tag="PKI07_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49582.1"
                     /translation="MENAVQSRLQSVQFGKAQRYQNLAILPLIAPADGACQYRTLGEA
                     LAAWDLAITEVSEAGSVPNLMVINRATQPVLLLDGEELAGARQNRVLNTSILIKGCSE
                     TRIPVSCTEQGRWAYTTRAFAESGNIMAFKARARKTRSVHESLESLGSYQSNQSDVWN
                     SIDKLYSKAGFRSATAAMSDLFKAQTEALLKCDEVFQLVPGQVGLLAFVDGQLVGAEM
                     ISRSAAYARLHAKLVRSYALEALMPSDPAPAGKPGEPAPAPGGSSAPPKPAGEADLTA
                     SARQFFAEVAAAKEQQFPSVGLGTDCRYEARNLVGTALVHEQEAIHAAFFRLDDSESP
                     SHRRRPFPRRHPLG"
     gene            25944..27062
                     /locus_tag="PKI07_00620"
     CDS             25944..27062
                     /locus_tag="PKI07_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOC49583.1"
                     /translation="MAKYKIAWLPGDGIGVEVLEAAKIVLDRLQLDAEYLPGDIGWEF
                     WRREGDALPPRTVELLQRVDAAMFGAITSKPARTAAAELAPELQDRGLSYRSPIVRMR
                     QLFDLYVCLRPCKGYPGNPLNFKEGIDLVVFRENTEDLYAGVEFAPVPPELARALGQL
                     ARPFAPFAKLPRDQYAISCKINTRAGSERIVRAAFDFARKHGRKKVTVVHKANVVRAT
                     DGLFLEAAKAVAKDYPEIPMDDANIDAMTMWLLKNPHNYDVLVAPNLYGDIISDLCAQ
                     MVGGLGFGCSGNLGTRLAVFEPTHGSAPKYAGQYKVNPIATILAVKMMLDWLGEAAKA
                     QALEQAVARVIQDGRVRTYDLGGTSTTLQMAQAIAEQL"
     gene            27059..27976
                     /locus_tag="PKI07_00625"
     CDS             27059..27976
                     /locus_tag="PKI07_00625"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOC49584.1"
                     /translation="MSAAASGPETRRVLLHEVGLRDGLQMEKQVVPLEQKVRWAEALL
                     EAGVDVVQLGSFVHPGKVPQMADTDALFQHFTAPGRKPAGATLSGLVLNEKGLERGLA
                     CGVELFCMGVSASETHSQKNTGMSVAEATTRIIAMAQQAAGAGKKVQLSVQSAFGCGF
                     EGPVPAERVLGIAGRFLEAGFRNLSLADTAGQGTPEQVEQLFGAILQRAPDAECTAHF
                     HNTYGLGMANCCAALRVGVKCFESAVAGLGGCPFTKVAGGNVCTEDLVYYLQRRGLRP
                     DVNRARLVEVARDLSRFFARDLPGMIYKI"
     gene            27973..29199
                     /locus_tag="PKI07_00630"
     CDS             27973..29199
                     /locus_tag="PKI07_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOC49585.1"
                     /translation="MTDSTHSTAPPRAALEGIRVLDLTNVLAGPFATLHLALLGAEVI
                     KIENPADGDLARKLGNVPRLNQELMGTSFLAQNSNKKSLTLNLKAPAGREIFKKLVAT
                     ADVLVENFRPDVMKRLGLDYPVLAQINPRLVYCAISGFGQSGPDAFKPAYDQIIQGLS
                     GVMAINGDERLNPLRAGFPVCDTVGGLNAAFATLAALFHRQRTGEGQFIDVALLDSIM
                     PLMGWVAANLLIGKQQPVLMGNDNFTAAPSGTFVTRDGFINIAANKQEQWEAVADLLG
                     VPELKTDPRFQERDQRKKNRKALTPLLEAKLKEKDTAYWVELLNRNDVPSGAILSLEH
                     ALSQPQVQHRQTLQPVAVEGIGQLQLFGLSAKLEKTPGGIKTPPPRLSAHTNALLTEL
                     GYSPADLDRFKAERVI"
     gene            29257..30537
                     /locus_tag="PKI07_00635"
     CDS             29257..30537
                     /locus_tag="PKI07_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOC49586.1"
                     /translation="MTVVEKILARATGRPSVKAGDVLEPKVDLAMSHENAALVINQFR
                     EIYQGTPLPPRIWDARRVAVIFDHRVPAESPKTASNQKKVREFIGAQGVGKFHDIRGD
                     VGGICHQVLPENGYVRPGYVVVGTDSHTTSHGALGAFAFGIGATEMASVWALGTVLNV
                     EVPPTIKVIVKGRFRKFVGAKDLILHIIGRISAQGANFKVLEFHGETIRRLPTSGRLV
                     LCNMSVEAGATAGIVPADEETARYLREEAGVTDPLEPVLPDAGANYESVLEVNVSRLE
                     PQIACPHTVDNVKPIGQVAGTRVNQIVIGSCTNGRLDDLAVAAKILKGRKVAPGTRML
                     IFPASAKIFRQALGKGYIGVFMQAGAVVMNSGCGPCLGIHQGALGDGETALSTTNRNF
                     KGRMGNPQAAVYLCSPAVAAASALTGVITDPRKS"
     gene            30569..31066
                     /gene="leuD"
                     /locus_tag="PKI07_00640"
     CDS             30569..31066
                     /gene="leuD"
                     /locus_tag="PKI07_00640"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOC49587.1"
                     /translation="MPNVVFKVGDDVSTDVIYPGRYMATVLPTETPQFAFADETAFNA
                     KLKARQVPPGSVLVGGRNFGCGSSREQAVSCLKGHELIIVARSFARIFLQNAINLGLR
                     MIVCPEIEAAEGDELELTAGAVVNKTTGRQFSVEPMPQSRQVIIDAGGLIPYTRKRLL
                     AQAAA"
     gene            31140..33713
                     /locus_tag="PKI07_00645"
     CDS             31140..33713
                     /locus_tag="PKI07_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HOC49588.1"
                     /translation="MRLLAGLLSGCAAVFVAGTLWAGGSGFNVIVVANQSSTNSVRLA
                     NRYCELRGVPPQNLWRMEGWTARTNTWSRANFQAFLYDPLLAAIAARGLTNQAEFILL
                     SMDIPYRVLDNDPYQTQNSTTAALFYGCKRDTAPPLICLPACCGLPAASSNSYAFSEM
                     PFREAPPDTALTNAFLTLMLTASNLASAERILSRGVASDSTFPTQAVYLAKSSDPARS
                     VRVVKFDNAIFDSRVRGDYPLVRTNTGSTAFTNLLGLQTGKTSFTLPTNAFVAGAMAD
                     NLTSNGGKIFEDRAGQTALLAMLDAGAAGSYGTVVEPCAYTQKFPDPRNYVYQKRGFC
                     LAEAYYQSLLNPYQGLLVGEPLAAPFARRGTADWNSLTNGAVLSGLALLTLDFTAADT
                     NLPLSQVDLFVDGRFWQRMADLPPAAGNELAVTLNGIPFNYTVPTNATVASVAADLAA
                     GLNGLTNLTHVQAWAVGDRLELQSLEVAQPGGAVTLSASTAAGSAAQLTTWLTPARDT
                     FLDTTATGGLSLLASNTPLAGDWLQLDFIKTNGECLSLSVTNPAAGTDIATLVSNLVN
                     RVKAEPALQSADGVLAADFADDTYCGIVAAEFTLYARSPGWPAAQFQVAFSASTNLLA
                     LPAGTNCLDANLNDLRPRNHLYVSSGAAALPVSFAFDTTRVPDGWHQLTAVAYEGTSV
                     RTQTRVARTVRVQNTALAATFTPLLAGTNATLDLPLQFSVAANATNIARLELFSTGGT
                     VGVVSNQSGAVFTAPVAALGLGLHPFYAVVTDADGNQYQTETISIRLVPPFRLRLDRA
                     PLTLSWEATPGVPYDVLAATNLAHGFAPVASLTASNILAVWPVPVPGEAAAFYRVRLS
                     P"
     gene            33796..34200
                     /locus_tag="PKI07_00650"
     CDS             33796..34200
                     /locus_tag="PKI07_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49589.1"
                     /translation="MPEIVAFVNSGAATITPAIVERMLRQLPQWKLEFTQIHEPLFPH
                     LVDQLEFLADVVEDVAEGVYKDLPYAAFCQAAFALIYAHKKVGIIPDTVLNLGRADDS
                     SVVRAVLIQNESAFALYAAAQNIEWSRITSQP"
     gene            complement(34217..35125)
                     /locus_tag="PKI07_00655"
     CDS             complement(34217..35125)
                     /locus_tag="PKI07_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49590.1"
                     /translation="MNRLWAFWLGCSTLIAGGETLPQRYASQGELILAPLAAAPFPHP
                     RRAEGHRYKEQFFPAREHYSDNTVAIFIPQGFRETGKIDFIVHFHGWKNQVEGVLTRY
                     QLIEQLIESQRNAVLVVPQGPRNAPDSFGGKLEDPGGFQRFMAEVADTLRQKSALTNK
                     SFAIGQILLSGHSGGYQVISAIVAQGGLEEHLREVWLFDALYAQTDRFLAWINRSAGR
                     FIDIYTEKGGTKAESERLMARLREQHTPFYAAKESAAGLADLRANRLIFLYSDLPHNE
                     VVHQRQQFRDYLKTSCCAARETAAAK"
     gene            35438..36295
                     /locus_tag="PKI07_00660"
     CDS             35438..36295
                     /locus_tag="PKI07_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC49591.1"
                     /translation="MATTLSSSERAQLAQTVEMFEGITQAEPHDYQSLEILKEAYSKL
                     NQEKDVINTSKRIAQAYVQLGQFASAKLEYEAILQRCPDDAEAKTALQEIASQADNLP
                     AEPAAPLEIAPAALRVSAAPAPAAPAAGQAAPGDVEDGRQTMYKLFVESRIIAPADFG
                     LCWPVVDPRVAPEGVIEPFIQALADRNILPADQSLKILSDQSRCAFIPLNTYDIDMDL
                     ARRFSAATCRRWCVLPFDRMSKSVLVATANPFNHQAGKELSKAAGSRLLWYLVPPHEL
                     IINLRKAFR"
     gene            36300..38039
                     /locus_tag="PKI07_00665"
     CDS             36300..38039
                     /locus_tag="PKI07_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOC49592.1"
                     /translation="MPPVKSFGERIVDVLVEDSLLTSKQVAEILELQKKEGTRLLELL
                     LKKSYVTDLDMAVAMGRVLNIPPINLAHLNIPLEVANLLPREIAHNHKVVPVSRLENK
                     LFLAMANPLNVLARDDVRDITRMEVAMLIAPEKAIQDKLAQIDAVRGGSIEDIIQDAQ
                     KKAIADEDADNVEVSREAIEEVNLDELAASTEEAPVIKLANLILVQAVKDRASDVHIE
                     PFDKTLRLRYRIDGVLVDATPPPKQMQLALVSRFKIMSSLDIAERRQPQDGRMRIRVG
                     GKDYDLRVSIMPTVHGEKVVLRLLDKANLSASIDKLGLDPDTFQQFKTAIDAPHGLIL
                     VTGPTGSGKTTTLYSALNELNDPVYNIVTVEDPVEFQIPGINQVPTKKEIGLTFANVL
                     RSILRQDPDIIMIGEIRDTETAEIAIEAALTGHQVLSTMHCNDAPGAITRLDDMGIAP
                     FLISSSVILACAQRLMRRICSHCKEPVTYPAKMYQDLNIDPSIFDGVTLYRGRGCERC
                     KNSGYAGRMAIIEAMTISDEIRKLIIARANSREIGKVAIGQGMRTLRMVALDRAQEGI
                     STLEQVLVLTSTH"
     gene            complement(38092..39801)
                     /locus_tag="PKI07_00670"
     CDS             complement(38092..39801)
                     /locus_tag="PKI07_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOC49593.1"
                     /translation="MKRSLLLPLALLLAAMPSPPARSAPPPGAGGEGVPGRAPAARKA
                     RLLAHWKLTKDGRDASGNGNHGIPHAVRFATAEHRKSAQFDGRQSWLEVPPSPSLRLG
                     TGDFTLAAWVRLPAEAEQDDVFGDIVSRFDPATRRGFNFRISLNAGVTTSQANARTVH
                     FGIDDGRLEPHWTDHGRPGEAVLIFGLATHDGELYAATCEPAPGRAGRVYRFGGGTNW
                     VDCGPPAPCNAVSALAEFQGALYAGVSRYRLAGSALPESPNTNAGGKVFRYRGGQAWE
                     DCGPLPGSETVGGLVVYRGQLYASALYPPAGFFRFEGGRRWTALPTPGRRVLALCVYD
                     GQLFASSYDEAHVFRFDGTGWTDCGQVGPLENTQTYSFAIYEGRLCVGTWKTGRVYGY
                     RGDHNWEDLGRLGGELEVMGMLVHNGMLYAGSLPLAEVYRLDRPGNWRPVGRLDQTPK
                     VTYRRAWTMSEFQGRLFCGTLPGGRVFSLEAGRNVTHDRALSPGWHHLAARRGGGRLA
                     LYLDGRRVAVSARFDSAPYDLALAQPLKIGFGPNDYFRGSLRDLRLYDAALTERQIAG
                     LAR"
     gene            39999..42758
                     /locus_tag="PKI07_00675"
     CDS             39999..42758
                     /locus_tag="PKI07_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49594.1"
                     /translation="MSPRVFCKTRPGASGRRVFHPLAETSAPRRKIVWRGLAFLLAAL
                     TPAVGAAPVMRTMKTASAGVPLLANGGFEETHAGRPVSWTAWRQGGRLAPGEGRQGTH
                     GVMCERRDGGGEYGFSQTLTLNRTNVAPFIIRGWSKAEQVSGSPDGGYALYVDILYTD
                     GTPLWGQTASFNCGTHDWEQRQFILLPDKPVRSLTLHCLFRGHTGKVWFDDAAVEEVA
                     APAGAFLFQGVPLQPSPREEPPRRAAPTRLATRDGLKLILRGDASASLRVGRTELGSH
                     APAGFLVRDFAAGSDFYGFAAEGSCAELGLRLAADFHAGADHIAVSGRLTDTTGRDRA
                     VTLLFALPLDATGWHWGDDARQSRRIEGGGEFANTVNLRSGANGKMSLYPLAAVWNER
                     AGLALGLDMDHPAQYRLVYHAGTRQFFIAYDFGLVPETARFPGGADFRFVIYHFDPRW
                     GFRAALQKYYDLFPRHFVRRVQREGLWMPFTDIARVPNPADFGFAFQEGAPNVAFDDR
                     HGIASFVYVEPMSYWLALPADAPRTYASALSVLTNDLRGARGAEKAEMAAATLTSGIR
                     DAADQFALYLVKAPWCDGGVFTLNPDPAIPASPEYPFTKAGVMHRQIEAAFRRHQPAA
                     TNTTPGAGLDGVYLDSLEMSSAELNYRREHFRTAQAPLVFDREGRPCQLMIFNTWTFA
                     RELARQMHDRGKLVFANSVLWRFAFPAPLLDVLGTEVNWLSQGEYHPDTDAVMNFRRA
                     LCRQKPYCLLLNTDYAKFTPDRVERFFQRCLFYGVWPGFFDPEAASKDPYWASGNKWY
                     ERDRPLFKKYIPWFRQLTAAGWQAIPHATCDNPRIQIERFGPDPGGAVWLTLFNDTPH
                     PQTGVITADLKALGLKRHPRARMLLSGTPAKPQPGGWQVSLSSQQAEILCLASESRHF
                     QGQTD"
     gene            43171..44742
                     /locus_tag="PKI07_00680"
     CDS             43171..44742
                     /locus_tag="PKI07_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOC49595.1"
                     /translation="MARKASRFRQICEDARALLDERAPRALTDFSKWQRFAHFWILVG
                     RSFVRNRCPVRASALSYVTVLALIPMLAVVMSISSALLKQEGEEQIDRFIDQFVTRIV
                     PPATFHTNRESASPQALNPPAATNLAVVAPDGSPPAVNSLVPADRGGETNSSLLPSLI
                     QDDRAVKARRAVARNIHDFIQNTRSGTLGITGSILLIFMAISMLSRIEDTFNDIWGVA
                     RGRSWFTRIVLYWGVITLVPLLLIVALGLATGPHLAGTREFLTAMPVVSKLTFQFLPV
                     VVLCLSFTLFYALMPNTRVRWPAALAGGLTGGLLFHLNNLISVLYVSRMVTTSKIYGS
                     LGLVPVFMIGMYFSWLILLFGGQVAYAWQNRATYLDERQAENINQRGREFVALRLMTC
                     IGERFTQGAPPPNALEIAEGLAVPTRLVKRITQTLAAARLVTETAGAEPAYLPARPLD
                     QITCHDVLLAMRASQGQECATRDEPARAEVYGEFHRIGEAERQAATAVSLQALVNRAQ
                     RLKQLPGPARPAAGE"
     gene            44963..46063
                     /gene="ald"
                     /locus_tag="PKI07_00685"
     CDS             44963..46063
                     /gene="ald"
                     /locus_tag="PKI07_00685"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOC49596.1"
                     /translation="MIIGVPKEIKDHEYRVGLIPSGAYQLIKRGHRVVVERNAGAGAG
                     YSDAEYVQAGATLVNDHAAVFEQAGLVVKVKEPLPSEYELLQPGQLLYTYLHLAASQP
                     LTEALMKARITGLAYETIEVNGRLPLLEPMSEIAGRMSVLVGGYFLGKHKNGSGVLLG
                     GVPGVLPGKVAVLGGGTAGINAARRAQGIGADVTILEVDLERMRFLDITLNTVHTLYS
                     NEVNLLELLPSVDLLIGAVLVPGAKAPRLVRRDMLRRMRPGSVLVDIAIDQGGCAETS
                     RPTTHDHPVFVEEGVAHYCVANMPGAYARTATQALTTATYRYLELIADRGLAEACQLE
                     PGLVGGINVMGGKLTHKAVAEAHELACSPPVL"
     gene            46105..46806
                     /locus_tag="PKI07_00690"
     CDS             46105..46806
                     /locus_tag="PKI07_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOC49597.1"
                     /translation="MKAPIPDNEAQRLAVLKEYHVLGTGAEEPYDDITRLAAILSRAP
                     FIAITLLDESRYWIKSKVGLERQEGPRDAALFCSYAILQSTPLIVRDARKDARFATSA
                     MVVRPPHICFYAGFPLINPEGFALGALCVLDRKPRVLSARQKEGLLILARHTMAQLEL
                     RRVSDRMANLLENIKLLQGLLPICAWCKRIRDDDGYWQQVESYVRQHLGTGFTHGICP
                     DCLEKLRPNKPKEKA"
     gene            complement(46839..47282)
                     /locus_tag="PKI07_00695"
     CDS             complement(46839..47282)
                     /locus_tag="PKI07_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49598.1"
                     /translation="MGPLQIETARDAHGQPGVWATLDPGAEPVFIARHQWIGVDLDGT
                     LARDDAADHFQPPYPLGEPVPEMIARVKSLLAAGVTVKIFSARACEPESIPIIQAWTE
                     RHGLGRLEVTHQKDYDLIRYYDDRAIQVLPNQGRPVSSLAAGKAG"
     gene            complement(47330..47704)
                     /locus_tag="PKI07_00700"
     CDS             complement(47330..47704)
                     /locus_tag="PKI07_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOC49599.1"
                     /translation="MNQTASLQPAIRVVMMPRDTNSMGTIFGGIILSHIDVAGAVEAR
                     RHSEKRLVTVAMHEVKFIAPVFVGDLVSFYTETVKIGTTSVTVRVTVDAKRSQPPRET
                     MRVTQAEVVYVAVDEPGRPVPL"
     gene            complement(47962..48570)
                     /locus_tag="PKI07_00705"
     CDS             complement(47962..48570)
                     /locus_tag="PKI07_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49600.1"
                     /translation="MKKLLFLAVTAGALLMLGSTANAITSISAIDVVQAPTGYFTPSD
                     SVKLSAPYYRWWNEDWGWTHSGAPFASVGTITSASLSISAFDVDWPSGQVDNIYAYNN
                     GVKTLLGTLSGLNNQWSYTTFVLGPEFYDDILSGLQVWMDIDSTHRFDTWAVSLAKSV
                     LEVNGNPLPDPNPDPRVPDSGATAALLGMFMVGLGCFRRIVK"
     gene            49150..50436
                     /gene="hemL"
                     /locus_tag="PKI07_00710"
     CDS             49150..50436
                     /gene="hemL"
                     /locus_tag="PKI07_00710"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOC49601.1"
                     /translation="MNLRPESEKLFAEALKYIPGGVNSPVRAFRAVGGQPFFVNRAQG
                     ARVWDVDGNEYLDYVGTWGPAILGHAHPRIIEAVRTAAMAGTSFGIPNPLEVRLAKLI
                     CAWVPSVQKVRLCNSGTEATMSAIRLARGFTRRDLIIKFDGCYHGHADSLLVKAGSGA
                     LTFGAPDSAGVPAAFTQHTRVLPFNDPEAVKAAFAAHKGQVAGIILEPVPGNAGLYLP
                     QPGYLEFLREITAANGALLIFDEVMTGFRLARGGAQERFGITPDLSCFGKVIGGGLPV
                     GAFGGRAEIMDHLAPLGPVYQAGTLSGNPLAMAAGLAALEELAASDAYAKLEQLGAAL
                     EAGMRAAARAAGVPMQFQRCGSLFCGYFTAGPVRNVSEALASDRARFGKYFHGLLAEG
                     IYLAPSPFEAGFLSTAHTPADIERTVAAAGRVLAAL"
     gene            50500..50913
                     /gene="ruvX"
                     /locus_tag="PKI07_00715"
     CDS             50500..50913
                     /gene="ruvX"
                     /locus_tag="PKI07_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOC49602.1"
                     /translation="MRILAIDHGSRRIGIAVSDELKLIAQPLEFIPAEPFTDFLARLQ
                     ELLREKEVELILVGLPRNMDGSHGPAAQKVQEFVAALRGHVGVPIRTWDERLTSAQAN
                     RLLIQGNVRREKRREKVDQMAAAILLQSYLDGLAG"
     gene            51116..52072
                     /gene="truA"
                     /locus_tag="PKI07_00720"
     CDS             51116..52072
                     /gene="truA"
                     /locus_tag="PKI07_00720"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOC49603.1"
                     /translation="MRKRGSARRQTGKVEGMRRPRRPETAVRPGNAPVCPGASPARMN
                     PLRLKLTIAYDGTAYEGWQAQKIGTGVQEKVEAALAKLFPSQPRVHGSSRTDTGVHAL
                     GMVAHLEIPRAECRMSPRKLVLAINAWLPEDVRVLAAARVPRSFHARFDAAGKQYRYF
                     VWNHPAQNPLARQVAWHVPRPLDLRAMRAAVPGLVGKHDFQSFAANPGYARESTVRTL
                     TRCALRRQGPWLTFILEGDGFLYRMCRGIVGTLVQVGLGKFSAEDVRRMLARRDRRVA
                     GMTAPAHGLVLWKVRYPRQRREAKGGPGRSRSGRRSRGPAAG"
     gene            52076..52540
                     /locus_tag="PKI07_00725"
     CDS             52076..52540
                     /locus_tag="PKI07_00725"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOC49604.1"
                     /translation="MHVVLLEPEIPPNTGNVARLCAATGSTLHLVEPLGFELNDAQLR
                     RAGMDYWQHVQWRRWPDWPAFRQALPRDARLWLVESNGPRLYTEARFAPQDYLVFGRE
                     TAGLPRKLLRENPDHWLRIPMFQPRARSLNLSNCVALVLFEALRQQGFPGEV"
     gene            complement(52601..53086)
                     /locus_tag="PKI07_00730"
     CDS             complement(52601..53086)
                     /locus_tag="PKI07_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOC49605.1"
                     /translation="MKNSHAVVLSELLSPATINLDLQSTDRNAVLAELVNQIPQFAQN
                     PEARQTLLNALYEREQLHSTSIGDGVALPHARNAIVGLVDQPIIVFGRHPKGIAYDTL
                     DHQPARLFFLLVAPTVTQHLAILARFNRLLRDPQLRQALLTAATPEEVLSRIRESETE
                     R"
     gene            53472..53717
                     /gene="rpmE"
                     /locus_tag="PKI07_00735"
     CDS             53472..53717
                     /gene="rpmE"
                     /locus_tag="PKI07_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOC49606.1"
                     /translation="MKEKIHPKYEDAEIRCACGNVIKTRSTKPRIIVGICNVCHPFYT
                     GQQKFVDTAGRVDKFQQRMVKTQAAQALAATRKKKKH"
     gene            53780..54859
                     /gene="prfA"
                     /locus_tag="PKI07_00740"
     CDS             53780..54859
                     /gene="prfA"
                     /locus_tag="PKI07_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOC49607.1"
                     /translation="MDLRPQIEKFAQRFAEVETALSDPKAFNNPQRAQELGKEYARLR
                     ELMGQGQAYAKVVADLAENRALLEAEPAESELALLVREEITRLEAEEKRLGQEIQRGI
                     LPPDPADSRNTIIEIRAGAGGQESALFAADLYRMYTRYAEQRHWKVEDLDSSASDLGG
                     YKEIIFQLNGSDVYRRLKYESGVHRVQRVPATEAQGRVHTSTCTVAVLPEAQEVEIEL
                     KPEDLEITVCRASGPGGQGVNTTDSAVQVVHKPSGTIVRCADQRSQQKNRARALMVLR
                     ARLLERKVAEENRKYADQRKEQVGTGERNERIRTYNFPQNRLTDHRIGLTLYNLPAVM
                     EGDLDPVLEPLMAHDLETKLAALAS"
     gene            54895..55515
                     /locus_tag="PKI07_00745"
     CDS             54895..55515
                     /locus_tag="PKI07_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOC49608.1"
                     /translation="MNASRLCPPPRPPGGIIFDFDGTLADTMPLHWQTWRLIAARYGL
                     SFSRERFYSLGGVPAREVLKMLSAEQGVALDPVAVAREKEAAYLALIDHVEPIPQVVR
                     VAREHYGRIPLAVASGGARPVIERMVDHLGLRHLFRAIVTHEDVARQKPAPDIFLEAA
                     RRIGVPPRACRAYEDTDLGIQAIRAAGMEAVDVRQLLAAANQPALA"
     gene            complement(55551..56366)
                     /gene="proC"
                     /locus_tag="PKI07_00750"
     CDS             complement(55551..56366)
                     /gene="proC"
                     /locus_tag="PKI07_00750"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOC49609.1"
                     /translation="MSKKLSLGFLGAGKMATALAKGFIQAGLVSARQTLAADPSKTSR
                     AAFTREVGAPTTPSNRVVAEFAQVLVLAVKPDQVAAVLDDIRDRFTSRHLLISIAAGI
                     PLARLEAGLGAGARLIRVMPNTPALIGASATAFALGKAAHPADGALVQQLFSAVGLAL
                     PVKETLLDAVTGLSGSGPAYGYLFIEGLSDGGVAAGLPREIATRLAAQTVLGSARMVL
                     ETGLHPGALKDMVASPGGTTIEGLHELEKGKLRGTLISAVRAATEKSRKLGQG"
     gene            complement(56789..59500)
                     /locus_tag="PKI07_00755"
     CDS             complement(56789..59500)
                     /locus_tag="PKI07_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOC49610.1"
                     /translation="MRIKPLSTDNLMARFLAGLARVVLRHPRLFLYPQLVLFAGCLVV
                     TLKYPGIEFNTSRNDLVGANKKYHQNFLRLKKEFPTQDDLVVVVESEDGEKNRQFVDR
                     LGAKLEAETNLFNHVFYKGDLKMLGSKALLLVPENDLADLRLILKDFQPFIAQFTRTT
                     NLVSLFRLICTQFRTAKQEASEANESLLKALPALERIITQATDSLRRSGAPPSPGITA
                     LFDPGEEAQQQMYITFAQGRIYLVTAQAPTEELNTAAVLRLRELVEETKLEVLGINVG
                     LTGEPVLELDEMAQSQKDTALASVVALALCALIFIYGYQETGRPVKATFCLIIGLGYT
                     LGFATLVIGHLNILTITFMPILIGLAIDYGVHLISRYEEELRRGEAMETALTRAMVLT
                     GQGILTGGLTTAGGFLAMGLTNFKGIQEMGIICGGGLVICLVPMMTLLPVLLLRGRQN
                     VVDRQLSPAAERRAWIENLWLRRPVLTAGVTLALCGLAATQLRKVYFDYNLVNMQSKG
                     LPAVEYEKALIASTPKSVLFGAVVATNLAQAGALERQLTNLSAVASVDSINGFLSEDQ
                     TRKLAVVGQIKQELAAVRFSEPDPRPVELQELSAVLYSLRGYLGAALEAVRAEQPELV
                     PQFESLRDAIQALRKEMLRGNDRQVAANALKLAEFQRRLFLDIRDTFQALRTQDDRAP
                     LSVADLPPALRDRFIGVTGKYLLMVYPQGDIWQREVQETFIRQVEKIYPDVTGTPVQL
                     YYYTALLKESYEQAAWYSLGAIGLLVFFHFRSLTSVLLALVPVAIGSLWLVGLMGWLD
                     VPFNPANIMTLPLVIGIGVTNGIHVLNRFAEEQSPSILARSTGKAVLVSCLTSMAGFG
                     SLMLARHQGIYSLGCVMTTGLATCMIAGLTFLPALLNLLVRPRVSTE"
     gene            complement(59585..60334)
                     /locus_tag="PKI07_00760"
     CDS             complement(59585..60334)
                     /locus_tag="PKI07_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC49611.1"
                     /translation="MAGHSKWAKVKHFKGALDAKRGKIFSKLSKEISIAAKIGGGDPS
                     MNPRLRMVLLKCRSANMPSDNIERAIKKGTGGGETANFEDLTYEIYGPHGVAILVEIS
                     TDSRNRTASEIRSLVTKNGGSIASQGAVTRLFHRKGQIIIARDAASEDQLMELALEAG
                     AEDFKAEPQGYEIITTPAQFEAVHKQIEAQGIKADVAGVTELPTLTVALPDSQAVAAV
                     DKLIEALEDHDDVKEVYSNAESPEGAAGSGG"
     gene            complement(60553..62265)
                     /locus_tag="PKI07_00765"
     CDS             complement(60553..62265)
                     /locus_tag="PKI07_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC49612.1"
                     /translation="MKRRRHKPGAPAPAQGRASGGPPQPETRAALPAAAARGRGRVWG
                     ISLLLALGAWLVFSPVWRFEFFRFDDPQYILDNPAVKAGLTGPGLAWALRATQAGNWH
                     PLTWVSHMLDCQLFGVHAGWFHLVNATFHALNAALLFLALRQLTGATWRSALVAALFA
                     LHPLRVESVAWISERKDVLSGFFFMLTLWAYARYARPPGPEERDPTVPARGAGRPPLP
                     ASLFYGLALVCFALGLLSKPMLVTVPLVLGLLDFWPLQRVPRRGQRAPGSTAGMSAWS
                     LLALEKVPFLTLALVQSGITLWAQKAEGAMATADQLRLSWRLGNALVAYVRYLRKMIW
                     PDQLAFLYPHPGAWPVEQVAGAAGVLLLVCLGMFWLGRRRRYWLVGGLWFLGMLVPVI
                     GLVQVGQQSWADRYSYLPSIGLLIILAWGLGDLAEKHRRAKGFVIAGAAVLLAASTVA
                     TARQLPLWKSTEPLYCRALDVALRDAVYRRAYETIPLYMELHLSFARDWAEVVQTAEE
                     KAQLVAYLRKWARLKPESAPVHLLLSEALARQGNWEEAVAEFNKAARLDPNVVRPPGA
                     GRSP"
     gene            62573..62753
                     /gene="ssrS"
                     /locus_tag="PKI07_00770"
     ncRNA           62573..62753
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKI07_00770"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT        11133 a        20691 c        20714 g        11000 t
ORIGIN      
        1 cgaaatggga aaagcatggc ctgcggggaa tgccggtgat ccgccacacc gcctcgcggt
       61 tcgccgggtt caaggattac tggaacgcct acgtgccggc ggccctgctg ccgcggctgt
      121 acggctacga gctgatgatg gcgccctaca ccatcgccca catgaagctg gccttgaaac
      181 tgtccgagat caatgcgcgc ctgggccagc cggattacca gttcaggtac gaggggcgcg
      241 ttcatatcta cctcacgaat gcgctggaac cggccagcgg ctttgcgaca cggcagtttg
      301 agccgttctt ccctgcgctt gcccacgaag cctccgccgt tggtgatgtc aagcgtcgca
      361 ggcggttcac cgtcatcatt gggaaccccc cgtactcgaa gatttcgtcg aacctgacgc
      421 cggagatgag ggccaccgtt gagaggtatc gttatcttga cggcgagagg atcaaggagc
      481 gtggggcgct gcagtttgaa ataaacctgc aggatgacta cgtaaaattc ttccggttct
      541 gtgaggaccg ggtgacttcc tctgggtgcg gggtgcttgg tgtgataacg aacaacgggt
      601 atctgagcac gccgacgctc cgcggaatgc gcgattctct gctggaaacc ttcgggtcag
      661 cgtggatcgt tgacctacac gggcacttgg ccaaagggga ggtcggtccg gagggcaaag
      721 cggaggagaa tgtcttcgac attgtccagg gggtttcgtt gtttctgggt gtgcgcactg
      781 gcgcggtgag aggcgatggc acggttcagc atggggagcg gtatggctcc cgaggggaga
      841 agtatgcctt tttggaagcg aataggtgca gcacaactaa gtttacccgg cttgagccct
      901 ctgcgccttt ctacctgttc gtgcctcatg acgctgactt agagcaggaa tggaaggggt
      961 acattggtat cggtgaattg ttcccaagga acagcgctgg gataatcacg gcgcgagatg
     1021 gccttgtcgt gtgtgaggat aggcgcgagt tggctgagcg ccttgagagg tttagccgtg
     1081 ctagggatga ggacgacgca atctacgccg aatttggatt ctccgagagc aagcgctttg
     1141 acctgaggga agcgcaggct gagctgcgga ggttaaggtc gtttagtgaa ccgattcgga
     1201 agatgttgca ccgacctttc gatgtgaggt tcgtgttctt ccacccaagc gtggtgtgga
     1261 gcctttcacg gcctatggct gagcaaatgg ttgacgggaa gaacttggct ttggttgcca
     1321 ctcggcaggt gacccgtcct cagttcgagc acgcgttcgt gtctcggcat atcatcgaga
     1381 tcaaggcgtg ctcgcacgac agaaacaccc agatctttcc tttgttcatt cgcggttcgg
     1441 atggggagct ggcgctttcg tcgggttggg cggcgaacct cgatacgggg gccgtgggca
     1501 ggttttcacg ggctcttggt gtgccattca atggcgcggc gtgcgatggg cgcgacggcg
     1561 atctgtcgcc gcatagcgtt ttttactatg cctacgctgt gtttcatagc cctgcttacc
     1621 gcacgcgcta cgcagagttc ttaaagatcg acttcccgcg tctgccgttg accgggaatc
     1681 tggagttgtt ccgcgcactg gcacggctcg gcggcgaatt ggttgcccta catctgcttg
     1741 agtcacccaa actggattcc cccatcaccg aatacctggg cggccgcgct cccgaggtcg
     1801 aaaaagttac ttgggccaaa aacaccgtct ggctggacaa ggcgcagacg gtcggattca
     1861 agggcgtgcg cgaggaggtg tggaatttcc acatcggcgg ctaccaggtt tgtgagaaat
     1921 ggctgaagga ccggaagggc cgggtgctgt ccgccgatga ccgcacccac taccagaaga
     1981 tcgtggtggc cttgtcggag acgatcaagc gaatggcgga gattgaccgg gtgatcgaga
     2041 agcacggcgg gtggccgatg aaatagaacg ctgccggatg aatactatga atgctgaagc
     2101 gtacctcctg aaacgatcgc ccagcgatgc gcgagagcat tgcggatagc gtttcgccaa
     2161 ggggacaggc accagcaggc ggaatgaagg atgccgctcc gaggaccgcc ccccaaggta
     2221 cggccgacgg aatgtcgtat tatttgcaaa ttaagcgtca ttttcccccc taaaccgggc
     2281 cgattcggat ttcgcctccc attcggcccc atgaaacaca taaatccctg gctttacggg
     2341 tgaaaggcat tggcggagcc aaaagctatc cgggggcccg cccgcccttg aaaaaccgct
     2401 ttttgccagc tttggcttca taactggctg tttttataga gcttggcaat tatcctattg
     2461 tccccgatca tcgtttcggc ctttccgggc atctgtatcc attccattag ccagatgcct
     2521 tggccacccc ctgccttcag gccgtgtaca aggggtgtac aagggatgcc caccgggaga
     2581 tcatggggtt ttagctggta cacccggtgg gcatcccttg tacacccctt gtacacggcc
     2641 tgatggcagg gtaaaatcag ggtaagttca aggtatggcc gacggaatgt cgtatgattt
     2701 ccaaagcaag tgcaggtccc gctcctgaat catctagata ttcggcatcg gggccaaggg
     2761 aacgcagagc ggattctcaa agttgagaat tgcgggcaaa aacgtgccgg ttgggaagtt
     2821 ttctatgtgg cggaattgtg gaatgagctt atttgctgtt atttcagatg cttgcgctat
     2881 ttacgatagg gaaatcaagt tccctcttcc tcgggagcat tatggaaggg ctggagcaaa
     2941 ccgcgggaaa cagccatttg cgttcgatga tgggccaaag gaggccgtct cagccctggt
     3001 tcaatccgag cggttcagtt tgacctcggc tatgaggaga tgcctgggct ggctgtaaat
     3061 ccgggaaagg gtgttttcgt cgagtcattg gccccaccgg cggcgtggcg gtcgcccgct
     3121 ctttgccggc aggctcctta ttcccggcaa ctctcccggt ggcttcgggt gtttcagaga
     3181 ttttgattcg tggcgggagg gaaaaaatta gaaaaaagca cttgcattcg aacggggtga
     3241 tattagattg gcggcagtaa acggcaatga ttgcctccag agcgaaaaga tattcgcggg
     3301 attcgtttca gcctcgaggg tttagcttga tcgagttgct gacggttatc tccacgattg
     3361 ccattctggc cgccctgctg tttcccattt tgaataaaac caaggtgaag gcgcagcgca
     3421 cgacgtgcct ttccaacctg cgtcaactgg ggtttgcgtg ggcgctgtac cgggatgata
     3481 atggcgatta cctggctgag tcttatcccg gcgatagaga ccccaacccc gatgtttggg
     3541 tcaaagggga tatgaccaaa ctccaggatg cgggcaatgc ggatttgctg cgcgagggca
     3601 agctttatcc ttatggcggc agcgcgccga tttatcattg tccggcggat cggggtgtct
     3661 cgattggcgg gaaaatagtc cccactgtgc gcagttattc gatgaacagt tttatgggcg
     3721 cacggccttc gagtgctcct atgattccct ccaccgccag agggtatgtt gatttctttg
     3781 cccggggctc ggatattccg cgcccggagc agatgtgggt gttgttggag gaggattccc
     3841 gcagcatcaa cgatggtttc ttcgtcaccg atccgaaggg gcagatctgg tatgattggc
     3901 cgacgatttc ggcggcgcgc cacaattaca gctatgcgct caatttcgcc gatggccatt
     3961 ccgcaacctg gcgcgtgacg gatccgcgca gcctgcgggt caccatcacc aagacggagc
     4021 agcccggcaa cgccgacctg caacgcctgg cgagcgccac tacggtccgg aaataaccgc
     4081 cccttgcggc ggtttacggc ggcggtttcc gccctcggac tatcctttcc cagccaaccg
     4141 gcaagcgccg gcggctccag agactcccgt ttacgccggc cccgtctatg gcccctgcgg
     4201 gaggagcacg acgcggtaga agcgccggct gaggctgccg acgttatcgg tcagcgtaac
     4261 ggtattgctg gtgggaacca cgatcttccc gagattcgtc caggcggcgg cctcaagccc
     4321 gggccgttgc tgcaggcggt actgttgccc ggggatggcg ctccaggtca gggccagcgt
     4381 gctgttcgag cgcgcggcgg agaggaacac cggcggcgga acgaccgtca ggcgcgcgac
     4441 ggcgctggtc acggcgccga acgcattggt tagcgtcacc cggtacaggc ccgcatcggc
     4501 gggggaggcg ctgtggagga tcaggttgcg ctgggccgag ccggaaatgt tggcgtcatc
     4561 ccacaggttg gtgccattcc gctgccattg ataaaccagc ggacgcgccc cggtgaccgc
     4621 cacgctgaat tgcgcgcagg ctccgggcac gaccgcctgg caggcgggtt gggtggtgat
     4681 tgccggggcg ccgtcgaagc tgaggcggaa cagggtgccc tgcccggcgt agcaggggcc
     4741 gccgcaggcc gtggtgccat acaggtggcc cgccgcatcc tcgatcagcg cggcccgcgg
     4801 cagcgcgcca tcattgcagc cgtcgaaata gagcagcgtg gtgaagacgc cgccgttggg
     4861 ggacacgcgg aagaccgtgc cataaccgcc gaggtaatcg gtgcgggtgg tgccgtagaa
     4921 attgccatcg cagctttgca tcaccccggc cgccgggcgc acgccatcct tcaggagcag
     4981 gtctccgaac gggtgcaggg tggtcagcgc gcccgccgga gtgatttgga agaccgtgcc
     5041 gctccagccc aggccgccgg tttcggtgca gccgtaaaag ttgccatcgc tgcccagcgc
     5101 gagcgtgccg acgggggaac tgccgtcccg gccgcccgta aacgagtaga gaggggtgag
     5161 ttcgcccgcg gcggtgatgc ggaagaagca gccctggtca aaggcgccgc cggcgggggt
     5221 caggccgtaa aggccgccgt ccgcgccccg caccagcgcg cccgccggcg atttgccgtc
     5281 caagccgccg gtgaaagcgt gcagcgtggt gaacgcgcca ttggtggtga tccgaaacac
     5341 gctgccataa ccgaacgcgc cgccggcgct ggcggtgccg tagaagctgc catcgtcccc
     5401 ggccagcaac ccggcggcgg gatgggcgcc atcgtccgcc gccgtgaacg catggagcgt
     5461 gtgcagcgcg ccggcgggtg tcagcgcaaa gaccgtgccc tggtcatgcg cgccgccctg
     5521 gaaggtggtg ccgtaatagc ggccatcggc ccccggcacc ggggccgcgc gggggctgct
     5581 tccgttggtt ccggcaaagg aggccagccg cacacaggcg ccatggatat cgcaggcgaa
     5641 tacggttccc tggttccaat cgccgcccgc ccgggttgtt ccgtagaaga gatccggccc
     5701 ggcgggggcc aacccgtcag gctcgccgcc gtcgaggccg ccgccgaacc agtgccgggt
     5761 tgtgaggctg gtgccggggg cgctcgcggg caccagggtc agccaggcgg aggcgctggc
     5821 gacgccaagg gaattgctga cgaccagggt atagaggcca ttgtcggctt cggtgacgtt
     5881 gctgatgacc agcgaggggg tcgtggcgcc gaagcggtgg caggtgtcgg ccaggctggc
     5941 gccgtccttc tgccactggt aagccagggg gaggtcgccc gccgccgtgg cggcgaacgt
     6001 gacggtggag caggccgccg gggtcaccgg ggccggcgcg tgaacgatta tgggcggagc
     6061 ggacagcacc tggaggaggg cggcgctgct gttcgtcgcg cccgcgggat tagccgcctg
     6121 gacggtgtag ctgccggagt cgttggtggt gacgccggcc aggatcagca cgcggtccgt
     6181 ggcgcccggc aggctggcgc cgtcttttcg ccattggtag gaaaacgggc gggcgcccga
     6241 taccgccacg ctgagcacga cggtttcgcc ctggtacgcc aactggctgg cggggggatc
     6301 ggtgatttgg agcgggccgc tgaagcccag gtggaagagg gtgcccaggc catattcgcc
     6361 gccgttctgg gtcgtgccat acagaaggcc gtcgtcgtcc tcggccaggg ccgcctgggg
     6421 attggcgccg gcgtagccgt tgaacgcgac cagcgtggtg agcgtgccat caggagccag
     6481 gcggaacacc gtgcccgcgc cgtagtcgcc gccgtaggcc gtggtgccgt agaaattgcc
     6541 atcgccgccc agaagcagcg ccgccgccgg gtagccgccg tcggcgccgc cgctaaagga
     6601 atgcagcggg tggagcgcgc cgtccgggct caggcggaag acggtgccgt gccggtgcgc
     6661 gccccccgcg gaggtcgtgc cgtagaagtt tccggccggg tcctggatca actcggcgag
     6721 cgggttggcg ccattggttt ggccaaaaga cgccaggctc ttgaacccgc cgtcggcggt
     6781 gatttggaag acggtgccat ggccgctggc gccgcctttg taggtggcgc cgtaaaaggt
     6841 gtggtcgcgg gcgcgaacca gccccgccgc caggtggccg ccgtcggcgc cattgctgaa
     6901 tgcgcgcagc gtggtgagcg ccccggcggg ggtcactttg aacgccgtgc cgcggccatg
     6961 cgcgcccccc tggtaggtgc tgccgtagaa attgccgtcc gcgcccggcg ccagcccggc
     7021 gcagggcagg ctgccgttgg taatgttgaa cgcgacgagc gtggtcagcc cgccgcccgg
     7081 cgtgaggcgg aagagggtgc cgttgtcatg ggcgccgccc tggtaggtgc tgccgtagaa
     7141 atcgccctcc ggcccgggcg ccagcgcgcc gaagggcgtc gcgccgtcgt cgccgcccgt
     7201 gaaggaatac agcggctgga cgaggcccgc gggggagagc tggaagacga ggcccgcgcc
     7261 gtgcgcgccg ccgttttgcg cggtgccgta gaaactgccg ttggcgcctt ggcgcagggc
     7321 gttcggattg gcgccatcgg agccgcccgt gaaggagtaa atcgtcgtca gcgcggtttg
     7381 cgggggggtg atggaggcca ccgtcagcac ggcgccgggg ctggccgcca ttccgtccgc
     7441 attggtcacg accactgaat acgcggccgc atcggcgggg gaaacgcagt ggagcgtgag
     7501 gtggggggtg tgcgacccgc tgacctgccg gccgtcgctc agggcggtgc cattccgctg
     7561 ccaggaatag aacagcggct ggccgccgac agcctcgacg ctgaaggtga ccgtctcgcc
     7621 cgcgaccacg gatcggccgg cgggcggggt cataatgacg ggccgccacg ggaaaacggc
     7681 caggaacgcc ggcacgctgg cgaccgcgcc cgcggcgttg ctgacgatca ccgaataagc
     7741 cccggcgtcg gcgggcgccg catgggcgat gaccagggag ctggacgcgg agccggaaac
     7801 ccggccgccg tccgtcaaat tggtcaaata gaccccgtta tcgtattgcc attggtaagc
     7861 gcaggacgcg ccgttggcgg gcaccacggt gaaaaccgcc gtcatatcct cgaagaccgt
     7921 ctggttcgtc ggctgcgcgg cgattacggg cggcgcgacc acgtccaggc tgaccagccg
     7981 gctttggctg aattcatcgg cggcgttggt ggtatttacg ttggtaaacc agagggtggc
     8041 cacgtagctg cccaccggca ggctgtccgc cgccgcggtc ggcgtcagcg tgacggacgc
     8101 cgccgggccg ccgtgggtca ggattccgct ggcgggcgag acgtggagcc acggcgcggc
     8161 gtgggtcacg ctccaggcga gcgccgcgtg gctgtcgttg gtcagggtga agccctgcgc
     8221 cgccgggcgg aaggggccgc cgagcgggcc gctgaaggcg ggcagggcgg cgggcgtcac
     8281 gcgcaaagct ggcggcgggg cgaggagcgc ggcaatgagg ttgctgcccg tgggcgtgcc
     8341 ccagccggtg cagaggtcgt agccggggcc ggccgggaac cggtcggggc cgcagcagtt
     8401 attggtatta ttgccggtgg tggtgtcgtg gaacgccgcg gcgtattcgg acgacttgcc
     8461 gatgcggtag agggcggggt tgatgaagcc gagggcgggc aggccgcggg cggccgcctg
     8521 ctggttggcc agggcggcaa agccggccca caagggcgcg gcggcgctgg tgcccgcgcc
     8581 gctgaactgt tcgccgttgt tcgcgatgag ccagatgtcc gcggccacgc aggccacgtc
     8641 ggggctgttg cgccaggtgt ccgagccgcc ccgggcggcc aggtcaattc cggtttgata
     8701 gccgggcagg gcgtggttcg tgccaaaccc gccgccgctg gccaccggct cgtccgggaa
     8761 atagctccag caggtttccc ccgcccaggc gccccccggc gattgggtgg tcaactgggt
     8821 gccgccgacc acggtgacgt tcgggttgtc cgtcggcgcc cagggcgggc tggcggcccc
     8881 gcaataagcg ccatcgtccc ccgaggcctg gaagaacgac tgcccttgcg cggcgaattg
     8941 caggaatatc tggttctcga gttcgaggga gggcgttcgc cagatccagg aggaactaag
     9001 ctgccgcgcc gcgacctggc cgaggtggtt ggtatcgttc gccatgcggt tgagcacggt
     9061 gcaggggctg ctggtgggcc ggccggcata gacgataatt tgggacaggc ccggagccat
     9121 gctcgacgcc atggcaatgt ccagcgccac ttcaatgttg ccagcgccgg gccggccgtt
     9181 gaagccgtcc accagcacgc tggtgacggg cacgtggggc agcccggcca ggttctggta
     9241 agccagcacg tcattggtgt aaaagccatc cagcgccagc aggccaacgg cttccccggc
     9301 tccgtccagc cccacgcccg gcgcataagc cgcccggaaa tcccggccca ggaggaaacc
     9361 gcgcgggccg gacccggctc ccgccggccg ggcgccggag tccgcgcccg cgccccgccc
     9421 ctgcggcacc caccgcaggt tcgccgggcg cggccgcgag aagttgtcca ggccgtcaat
     9481 catcagcacc cgcgtttgca aatcgaggga gggctccgca tccggcgcat aaaagttccg
     9541 cgcctccgtc gggtggcggt aggactgcat ccggacgcga aaggcccgtt cgatgtccgc
     9601 caccgcgccg ctcacattca acagcatgcg gttcgggtgc gtggcggtga tccgcaggcc
     9661 gcgcgcgcgg gcaaaagcga ttaccgcctc gtaatcctgc cggctcggcc cgaaccgctc
     9721 cacgaactcc gccggggcga ggtagtgctg atattcaggg tggaacggat cgtaaatctt
     9781 ttcgagcaag tcgcgcaggg cctcccggtt gcgcaaaggc aggccgatgg tcagctcaag
     9841 ccggttcgtg cccggcaatc gtcccagcgc cgcccaatgc ctcgcccctc ccggcacatg
     9901 cccgctcagc cgctgccgct ccgccccccg cgcggacatc ccggccggcc acagcaacag
     9961 ggcgcagcag gcggcggcca gcattgaggc cccccttgcc ggacgccgtt ttgccgtgcc
    10021 ggcgtttgcg gaaggcgggg attccggggc ggctttgggg cgtgcaatca tgatattccg
    10081 ggcattactg gctgatagta aaacatgcgg gcgcgggtgg ggcaaggctg gaggccggcc
    10141 gccccgggcc ggatactcat tcggcctgac ggcggcgcaa gcatggcggc gccgtttccc
    10201 ggagaggcgg atcgttgcgg gctggcgagg ctaaacccac tgccaccgcg ccgccagcgc
    10261 gcgccggagt tcgttcgtcg ccgccaggcc ttcggcgggt ttgatgactt cggcggcatg
    10321 gatcaggtag gccaaggcca actcgcgctc gcagccccgg atgcccgcgc gctcagcgta
    10381 agccaggaac gcttccgaac gaagcaggcc ttccatgcgc tgaaccagcc ccgcgacggg
    10441 caagcgctcc acgaggatgg cgggttgaag cacataatga aaccaatccc agccgggaat
    10501 gccggccagc tcgccccgct cccagtccag caccgtccag gcgcccgagg cgggcgagac
    10561 tttgatattc cagggggcga agtcgccatg ttgaagcgtt gccggaacgg tccgccccgg
    10621 gagccgttga agcagcgggt ttggcagctc gcgggcctgg acggcgcggg tcaatcgctg
    10681 ccaatccgcc gtctcgccca acgcgagggt ccgttgcgtg tccacccacg aagtcagcag
    10741 cgccggcagc gcgccctcat cgcgcgggcg gggtgaatcg ccgggcgcga aatccatcgc
    10801 cagcgcgcgc tgccgggggg actggaagtg cgcgcgcaac cggggcacgc tgccgaggtt
    10861 cgggggcatc gccgccagca gcgcccgttc cttttcgacc agggcctggg cgcgctcgct
    10921 caggccggct ttgactactg caaccgggtt ttggccggcg tcgaacacca ggatcaggaa
    10981 gcgctggtca tccttggcgg gatttcccgc caggattccc atcgtcggca gcgccggctt
    11041 cggctccccg gccagcgagg cgatgaaccg cacaaagtca tcctcgggag cgatggcaaa
    11101 gctgaggggc cgggttccca ggggaatcgc cccgcccacg gcccagcgca aaagcgcccg
    11161 ggcggcgcgg gcgcgggggg tctgcgccgg gtaaaggctc aaagacagcg ccgcggcgcg
    11221 ccgttggcgg ggcagcagca gaaagggccg cccgtgtttg cggaccaggc gcaggttcat
    11281 ccacgcgccg gcaggtgccc ccgttgcggg gccgaacagg gcttcccaga aatccggtgg
    11341 ctcgggcatg atcttaaagc gggggtttcg cccgttcggt ttcgggcaaa ggcgggccgg
    11401 gccgggtgcc caggcgccgc tgcgtccgct ccgccatata atcgagaatc acgcgggcaa
    11461 tatcggcccc taccttttcg atgggctggg cggcgtcaat gatgcgtccg ttggggaggc
    11521 ggctcaccag cgcccgatac gcgcggcatt ggcgttccgt ctcgggccgc gggacttcct
    11581 gtttgcggct ctgcaacacc tcggcgggcg catccaacag gatcaccaaa tccggctttt
    11641 tcaggaaagc gtagcccagc cgggcgaggc ccgggggcaa ctgcaggcgg tagcggccgg
    11701 ggtccacaaa gaaatcgtag taatagcgat caaccagcac cagcccgccg cggaacgtgg
    11761 ttgggcggaa ggacaggtgc gagccgagaa agaactccag ccagtgaaag agaaaataaa
    11821 cgagggatgc gaaccggttg cgcacgggct ggccatgcgg gttggtcgcg ggtccgcgcg
    11881 cggcccgccg gcgggcggtg aacaccggcg gcttccaatg aaaatgccgc cccttgagcg
    11941 ggctgaaggt gcaactcaac tcatccatca acacccgggc aatggtggat tttccgcagc
    12001 cgtccgctcc gcacagcaca acggtcaggc ccggggggcg cagaaaacgc agcgtgagcc
    12061 gcttcgcatc ggccagcacg gaacgcaggg tccgcccggg atgccgcgtc agttgccgga
    12121 gaatcagcgt gcgccgcaaa gcgccggcgg cgctttcgat cgccgtccac cgctgctccg
    12181 ccccggcggc cacgaggaac cgcgcgcgcg cggcgccgta ggtctgcgcc agcaatttcg
    12241 tcatcgccgc ggcttccgcg cggcattggg cggtgatgaa cgcccggtat ttctccttta
    12301 cccggcccgt gtagatcagg aaggagagca ggctcgtggc ggcttcgtcg gcgggatgcg
    12361 gcacggcaaa caactcgcgc cggacgcggc gttcgagaaa gccccggcag ccgagaaaat
    12421 caaacgcgcg ccacttcgac gcgtcaaaca aatcgaagtg cgcctgggca tgggagcgcc
    12481 ggctcgacag gtagagggcc accggcatga actcggcgcg gttgtgcagc cggaagcccg
    12541 cttcccgcgc cgccgccacc agcgtttgct ccgcgcgccg gcgctggccc ggcgccacca
    12601 gcacgtcaat gtcattggtc gtaaagtcag gcagcccctc gtagttgcgc aggatcagga
    12661 aagccatgcc gtcctggtcc caggcgcgaa acaccgcccg caccagttgg ctgatttcct
    12721 gtgcttccgc gctcatgtgc cacggcgctc cgccggcccg gccggcggct ttcgcctccc
    12781 cggttcaatc gcaaaacaag cggcagaaca attctttggc gcgggccttc ccttcctccg
    12841 aaagcatgac ggatttggcc ttgcccacgg ggttgctgat gtagcgtttg gcatggaggc
    12901 gggccagcgc gtcccagtcg tgggtcttcc aggcgcgcgt ggacttcagg gcgccgctgt
    12961 tttccttaaa gctggtcagg tgcagcaggg ccagaatcgc atcatctatc ttatcatagt
    13021 tcggcgtcat aaaactcaaa ttcaggacca ttaaccggcg cacggggcgc gtcaaaggat
    13081 agcttcgcat cttgactttc gccagtcgtc ccctagccta gcagacaaat cgtatgaaac
    13141 caatccagaa agcatccgtt tgtgggcttg gcaaacttgg cgcttgcatc gcggcgaccc
    13201 tggcccagcg caggttcgag gtcgtgggca ttgacattga cccggaaaag gtggcccgga
    13261 tcaacgcggg ccagcccccg gtcgaggaac ccctcctggc ggagaccatc accgccggcc
    13321 gggcccggct ccgcgccacc caggactacc gcgaggcggt cgccaccgac gtgactttct
    13381 tcatcccccc ctctccgagt ctgccggatg gcagcttctc caacgagttc ctgctgcgcg
    13441 caatgcagcc catcgccgcc gccgtgcgcg cgcaaggcaa gtccggccac ctgtttgtct
    13501 gcagttcgac caccactccc ggcgcggtgg acacggtcct catcccgatg ctcgagcgtg
    13561 agaccggcgg ggtgtgcggc cgggatttcg gtgtgtgcta caaccccgag ttcatagccc
    13621 tgggcaacgt cgtgaagggc ctgctcgagc cggacctggt gttgatcggc gaatccgatc
    13681 cccagagcgg cgcggtgctc gaggccctgt accggcagta caaccagaac cagcccaaca
    13741 tcgcccgcat gtccatcatc agcgccgagc tgaccaaaat ctcccttaac agctacgtga
    13801 cgatgaagat cagcttcacc aaccagttgc gcatgattgc cgagcagttt cccaaggcga
    13861 acatccatca aatcctcgat gccatcggca gcgacagccg cgtgggccgc aaatacctgc
    13921 gcgcgggcct gagctatggc gggccctgct tcccgcgcga caaccggctg ctggcctaca
    13981 ccgcccggca gttgaacctg gacgcgccgc tggccgaggc cgcggacaag gtgaatcacc
    14041 gcgccaacgc ggctttgttc gagcgcgttt gccggctggt gagcaagacg gacaccgtcg
    14101 ccgtgctcgg gctgacctac aagccggata cttacatcac cgaggaggcc gcgggcctgt
    14161 ttctggcgca gcaacttaag cgcaacggct acaaggttgt ggtgcacgac tacggcgccc
    14221 ggccggagat tgagccgagc gtgatcgagt ttgaacacct ggccgacccg gcaacgctcg
    14281 cccagcgcgc cgacatcaag ctggccgtga tctgctgtcc ctggccccag taccgctccg
    14341 tgaagtttgc ccccgcgacc caggtcctgt cgccctggaa gctctgatct gcgggcgggg
    14401 aacggcgcta atcgagcgaa tacctccgga ggcgggtcac ctgccccagc gcgcgcagga
    14461 taaacagcgg gttgttcttg aggtagcgct tccagagccg gcccgggtcg ctgcccaggc
    14521 ggaacagcca ctccaaaccg ctgcgctgca tccagcgcgg cgcctgccgc atccggccgg
    14581 cgtgaaaatc aaaggccgcg cccacgccga agaacagcgt cgcctcgagc cgctgccagt
    14641 attgcgccat gaacttctcc tgtttcggcg tgctcagccc cacccaaaag atgtccggcc
    14701 gcagcttgcc cacgaggtcc tgcagttgct gctcttcggc cgggttcagc ggccggaacg
    14761 ggggcgtgta ggtgccggcg atctgcaggc ccggaaaccg ctgctccagc cgcgccttga
    14821 gcgtgtcggc cacgcccggc gcgccgccgt aaagaaaatg cgtgtagccc cgcgtccgcg
    14881 tgaactcgca aacccgcagc atcaagtccg gcccataaac ccgccgcatc tgccggaatc
    14941 cctgccagcg gcccatccag accgtcggca tgccgtcggg cgtcgtcaga aacgcacggt
    15001 tcaaaatcgt ccggaaggcg gggtcggcct gggcttcgct tacgccatgc acccccgtca
    15061 cgcatacata ccccttggtt ttccgcgcca gggcctcggc gatcgccgcc acggccgtat
    15121 ccaggttgat cgcgctgatg cccactccca gaacattggc gcggccggct ttgacttgat
    15181 tttcagggct ttgcaacggc aggcaaaata ggctcactcc gaacgccgcg tcaaccccgg
    15241 gcgcgggagt ccaaatgcgc ggacgcctcc gcggcccggt ctcaatccgg ctcgggagga
    15301 tgctgcgcga ggtaagcccg ccacaaatcc agggccacgt aaagttcgag gaaaacctgc
    15361 gggcgcagcg cgtaataacc ggccatgacg tgggtttgcc gctcatccag tcccgcgtgg
    15421 aatgacgcgg aaacaatcat caccggcgcg tccggcgggg gtgccaggtg gtcccaccag
    15481 ccaacccgct tgagattgcg cagataccag ggcaacggcc agtagtcgtc ttccgcggcc
    15541 atcaccttca ccggcatttg ccgcccctgc ggatgcgccc gcgcgagcgc ctcgacctgg
    15601 cggaccagcc gcagcaggtc aggcgaggtt tgcgcataga cataagggtt gcgccggtcg
    15661 gcggcatact ccccattggc ccggacggcc tgccacgcca gatgccccgc ccccgccagc
    15721 agcaaaagcg tgaccaccat gcgccccgcc cggcgccggg cccggcccgc cagccaggcg
    15781 gcgccgacgc ccgccagcaa aatcatgccg tgccagaagc ccagcaggca ccacggggtc
    15841 ttgtaggaga tcacgccgta agcggccatg agcgcgaaag catagaaggc caggaatcgc
    15901 acaaacccgg cgttggcatc ccccaggccc cggcgcacaa aagcggcgcg ggcgcccgcc
    15961 accgccagcg ccaggatcaa agcctcgctc cacaccggcc ccggcgcggc ctgaaagaac
    16021 aacagccgat gcagataaaa actccacggg tggacgtgcg gcgaagcccc gccggcgcgg
    16081 gcgagccagg gcaaataggt gcgcacggaa tcgagcagcc cggcggcgtt cgtaaagaaa
    16141 gaggaaaaca gcagcagcgc caccaacagc caggcgcccg ccgcggcggc cacatggctg
    16201 agctggatcc ccccgggcct ggccgacgga gcggcgccgg catccagcca gcggttccag
    16261 agctgattca gccccaacgc cagcgcggcg gccgccagcg cgatgacaaa cgtctccttg
    16321 gtcgcgtgca tggcgcccac cgcggcgccc gcgagcagca gccagcccat tcggcggcgg
    16381 cgccaatagc gccacccggc ggccagcgcc aggaaggtga aaaagaccag cagtacctcg
    16441 tgaatataat accggctgta gaaaacaacc gccggggaaa ccacggtcca cagcgcggcc
    16501 caaaccacgg cgcgccgtcc cagcccatcc gcaatcagcg gcagcagcag caacagcccg
    16561 acgccaaaaa ggacgggggt aaaccgcagc cgcctctccg tgaaggaatc cgcatccggc
    16621 ggcgcgccgg taaggcggct gatcgccagg gtggcatagt aaagcgaggg cccgtgatgc
    16681 tcattcgggt cataccgata accctggccc gaccaaagcc gcccgaactt gacgccgttg
    16741 acggcctcgt cgttgtgcag gggacgccgg cccaggtcaa ggctccacag ggccagcacc
    16801 agcgcggcca gcagcgcaat caacggcccc gggccccgca tccgctccct ccctcgacaa
    16861 cctcccggaa ccgtgccgac ccgcgccccc ggcccagggg agccggatgg gcgccggagc
    16921 atgcgccccg gccgtcagcc gggggcgggg tgcccaaccg cgcgccggtt cgccccgcgc
    16981 gaattcgccc acgggcttgc cgaactcgga tttcgcgggt catgcgcgcc ctccgcgggc
    17041 cgccgccgct tacttggcgg gacggccgta aacctccact tcggtgtact cgttgagggc
    17101 gctttccgtg ctgcccttgg agtagaaacg aatatagcgg gccttgacgc ccttggcgtc
    17161 aatgagcttg ccttcgtggg tctcgaaata ctcgcgctgc gtgcccacgc ccaggccgga
    17221 gctgttatcc gcgtcattat tgaaaaccgt gcgcacgttt tcaatgaagt ccgcgtcatc
    17281 ggccacctgc acaatcacgt cgcgatacac cttggccatg ttatgcgcat gccagatcac
    17341 caccgcaaag atttcctggg ggctgcccag atccatttgc acccattgcg tgcctttgcg
    17401 caggaagata atgctttggt cggaggcatc cttgtcgccg tcggtaatcc gggccaggat
    17461 gtcgctggtg gcgttcttgt cgctgctcgt cagcttgctg cccggcgcaa ggttcttcag
    17521 cccgcggggc accatcatcg gcgggcgggg cttgtcggac aacggctcga cgttggggcc
    17581 cacgtctacg tccttgggcg tccccttgaa ggcgggcgcc ggcaacttca gcgccagcgg
    17641 ctccaggttc gcgttgtccg ccgcctggga aggcgtcacg gacaggttca ggacggcgat
    17701 ggccaggcca gacagcaacg acagcgccat cgtccgtcgc agccaggtgc gagtggtatt
    17761 catgttattc gtcattttcc aatagttaaa cccaaccggc gccgggcgac aagcccgatt
    17821 gccggggggt cacagaaaca gacgaagatg gcgtcccgtt tattcgacct cccggacgcc
    17881 caccgccccc caaacccgcc gcccggccac ttttgtcctt gatcatggcc gcgcgcgccg
    17941 gttacttggt cggacatgct agcccagaca tttcacacct gcaaggctgg agcgcgggca
    18001 tcttgcccgc ttacagcctc ccaacgggct aagcgggcaa gatgcccgcg ctccggcctg
    18061 cgggtatttc gtgcggggcg tgaaatatcc cggctagtag aaaggcaatc cgcgccccgg
    18121 ccgccccggc atggcgatgg gcgggcgcgg gactgaaacc cgaaagccag gaattatggt
    18181 gacgcttcct taccccacca acccgaacct gcaggcggtg ccggtgtatc agcccggccg
    18241 ccccatcgaa gaggtcgccc gcgagctggg actgcccgcc gatgacatcg tcaagctggc
    18301 ctccaacgag aaccccctcg gcccctcgcg gcgggccgtg gccgccatgc gcaaagcgct
    18361 ggcccaggcg catctctacc cggatggcaa cgcccattac ctgaagcaaa agctcgccgc
    18421 ccggctcggc gtcaccccgg cccacctgat cctgggcaac ggctccaacg aggtgatcga
    18481 gttcctcggg cacgcgctca ttgcgccggg aaccgaggtg gtcatggccc aatacagctt
    18541 cgcggtctat gccatcgtca ccgccctgtt cggcggccag ctcgtcgttg tccccgcccg
    18601 gaactacggc cacgacctgg acgccatgcg ggcggcgatc accccccgga cccggctcgt
    18661 gttcgtcgcc aaccccaaca acccgaccgg cacctgcgtc gccgccgccg acctggcgcg
    18721 gttcgtcgcc gcggtgcccg agaacgtcgt catcgccctc gacgaggcct acttcgagtt
    18781 cctggaccag cccctggacc tgacgccgga aatccgcgcg ggccgcaagc ccaacctgat
    18841 cctgatgcgg actttctcca agatctacgg gctggccggc gcgcggatcg gctatggcat
    18901 cgcgcatccc gccctgattg ccgagctgga gaaaatccgc cagcccttca acatcaacgc
    18961 catcgcccag gccggcgccc tggccgcgct cgacgacgcg gcgcacgccc aaaaaacccg
    19021 ccgcctgaac gcgcgcgggc tcaggttcta cgcgcgcgct ttccgccggc tggggctgga
    19081 atacatcccc tccgccgcca acttcatcct ggtccgcgtc ggcgacggcc agcgggtctt
    19141 ccacgaaatg cagcaactgg gcgtcatcac gcggccgatg ggcggctacc agttgcccgc
    19201 gtggattcgc atctccatcg gcacgccccg ggaaaacaaa cactgcctgg ccgccctcca
    19261 gaccgccctg gcccggacgg cggcggcacc cgcctgagcc ctttcccctg agctaacgtg
    19321 aaactcaccg acctgcgggg cattctccgc tacatcccgc aattccgcga caagacgttc
    19381 atcatcaacg tggacggcgc gatcgtcacc gacgagaact tcaccaacct cctcgtggat
    19441 attgccgtcc tgcgctccct gaacatccac gtcgtgctcg tgcatggcgc ctccgcccag
    19501 attcgcgcgc tggcccgcga aaccggccgc gccccctcga accttgacgg aaccggcatc
    19561 accgacgaag ccacgctcca gctcgcgctc accgccgcca accggctcac gcacgaaatc
    19621 ctcgagggcc tggccgccaa cgacctccgc gccgcctgca ccaacgccgt catcgcccac
    19681 cccgccggca tcctgcacgg ggtggaccag cttttcaccg gcaaggtcga gcgcgtggac
    19741 accgagctgc tccagcggct cctcgcccag ggcatcatcc ccatcctccc ccccctgggc
    19801 ttcgacggcg aaggcaagac ctaccgcgtc aattccgatg gcgtcgcgct ggccgccgcc
    19861 gaggcgctca aggccgccaa gctcatgttc atcaccgccc atgacggcat ccgggtcggc
    19921 gaacgcatca tccgccagat gcccctggcc gacctcgagt cggtcctgac gctccagcgg
    19981 caggccatcc cccccgacct gctttccaag gccattcacg ccgccgccgc ctgcaaagcc
    20041 ggggtgccgc gcgtccacgt catcaacggc tccctgccgg aaggcctgct ggccgaagtc
    20101 ttcaccaacg agggcgtcgg cacgctcatc tacggcaacg agttccagca aatccgccgc
    20161 gccctgaaaa aggacatccg cagcatcctc aacctgaccc gcggctccgt ggaaagcgag
    20221 gaactggtca agcgcacccg gcccgccatc gaaaaacaac tcgccgacta ctacatcttc
    20281 gaaattgata aaaaccccgt cgcctgcgtc gccctgcata cctggcccga actgggcaaa
    20341 ggtgaactgg cctgcctcta cgtcagctcc gcccacgaga accagggcat cggccgcaag
    20401 ctcgtccagt ttgtggaaaa caaagcccgc gagctgggcc tccaggaact cattgcgctc
    20461 tccacgcaag cctttacctg gttccagtcc aaaggcggct tcgccgaagg cacccccgac
    20521 gacctgccgc ccgcccggcg cgagaaatac gaccagagcg gccgccactc caaaatcctg
    20581 ctcaaaaaac tcaaataacc cgggccgcgc gcggccgcca gccgggcgcc tccctttgcg
    20641 cacacgttca ttcacccgga gtcccattcc ctgatatccc ctatgcggcc ttatttcccc
    20701 accctcctgt tgctgatttg cagcaacgtc tttatgacct ttgcctggta tgcccatttg
    20761 aaaaacctgg ccgacaaacc ctggctcctg gccgcgctgg ccagttgggg catcgccctg
    20821 tttgaatacc tgttgcaggt gcccgccaac cgcatcggcc acgaggtgat gagcgtggcg
    20881 caactgaaaa tcctgcagga ggtggtgacg ctgacggtgt tcgtgccatt cgcgattttc
    20941 tatctgaaag aaaaacccgg actggacttc ctgtgggcgg gcttgtgcct gctgggggcg
    21001 gtgtttttca tgttccgcag caaactgatg acctgatccc gcttccgccg ggtgcgcccc
    21061 ggccgtatcc cgcgggccgc ggccgccgcc cgggcgcacg gcgcttcact cggtcgcgcg
    21121 cccggtaaag cccgccagca tggccaggat cacgtcctgg tcggcgcgca cggaggtcgc
    21181 gcccgcgtag cgattgacca tgatggagaa gagcaggcgc tcgccggccg ccgtggtgac
    21241 gtggccggag atcgaactcg tccagcgcag ggagccggtt ttggcgcgca cgttgcccgc
    21301 cgccggcgtg cctttcatgc ggttgcgcag cgtgccgtcc acgcccgcga tcggcagcgc
    21361 gtccaggtac gcctggctct ccggatggcg gtgcatgaat tgcagcaggg tcacggtggc
    21421 gccgggcgtg gtcaggttgt cccgcgaaag cccggagcct tcctcaaaga tagtctggcc
    21481 ggccgggatg ccggcctgcc ccagaaaccg gttcaactcc cggatgccca ggtcttccga
    21541 agtcgcgcgg gccgcggtat tggccgtccg cgattgctcc cccacatgcg ccagcagcag
    21601 gtcggtgtac agattctgcg agggcttctg cacctcgcgg gccagctccc gcatgggccg
    21661 cgacgccacc gcgcccaact cgacctgccg cccgtagtcc accggccggg cttcccggtc
    21721 cagccagttg acgctgcgcg cccggcccgc gaccgcaatg ccccggcgga tgagggcctc
    21781 gcggaaaaac ccggcgaaca acccggcggg gttatgcacg gtgacctcgt cagtgtagtt
    21841 cgtgccgtcc accggcattt ggccggagac gtaaatcagg ttttcgcaga gcgggtgata
    21901 aagatggatg ctccgtgcgg cgcctttggc gaccgtgacg gcgcggttgc tgaacgtgag
    21961 ccaggccgtg gcgggctgca gcgacagccg gcatggcgct cccacggcgg gcccgggctg
    22021 gacgcgaagc cgcagcgtgt tgtcgttgat ggtgagggcg gaaatctccg cgccataata
    22081 gtactccatg tcgtcccaca tccagcccgc gccaaacggc gggccgcgga agtagctctc
    22141 gtcggccacc acatccccgg tgatgcgttt cacgccggcg ttggtcagcg cgttcaccag
    22201 cggatccagg gcccggtaga tgtcgccgcc gttcaaacgc tcattgaagc ccgggtcacc
    22261 gcgcccatac accaccaaat cgcctgcgag cgtgccggag gaatccggcc gggccgcggc
    22321 gtaaagggag gtccggatcc ggtaatcccc ccccaaccgg tccagcgcca gcgcgacggt
    22381 gtagagcttc gagttcgagg ccggggtgaa cagcttcccc gcattgtgct cgaaaaccgt
    22441 cgcgccgctt tcgagcgcga caatcttgac gccccacaac gcggcggcaa accgcggctg
    22501 ggccaggtga tccgtcagcc gttgccgcaa ttcagccagc gtggccggcg gcttcagttc
    22561 ggcggtcttt tgcggttggg cgggcgcgga gaaaacgagc gccgccgcgc cgaatacgca
    22621 aataagggcg gccagacgcc gttcgaatcc agtaaagaca gggcacttta gttgattcat
    22681 ccccataaga ttgggtgcca attcggccag gggcaagccg caatcgctgg ttaacgcccg
    22741 gtttttacct ccctgaccgc cgaattccaa gcccctcctt cctatcggct gcccaactgt
    22801 gccattttgg cacagttccg cgcccggcgc aggactcgaa tgtcccagcc aaaccgttcc
    22861 tcccgaacac ctcaaaaagg caaaaacaat cccccatcac cctcctcccg cccaaccaat
    22921 ccccaaccga cgcccccgcg cctcggattc tcaattctga gaatcctgcc ggcttcctcc
    22981 ctaccttcga gccgtgtaca aggggtgtac aagggatgct caccgggtgt acgtaggatt
    23041 ttccattgct gatccgatga gcatccgatg ggcacccctt gtacacccct tgtacacggc
    23101 taattattag cccggaatgg acttgggatt ggctggattt cttagcaaaa ctggggataa
    23161 ttgacgccaa agccggtttc cggctccatt tcgctatgcg gggcctttgc caccgctgcc
    23221 gtggctacga tttatctcaa accggggctc ttggaggact ttttcttcct tttgggtctt
    23281 ctgcgcccgg ttcggacggc ctttggcctc ccgggtcagc tttgcgcgcc ccctccgtat
    23341 ttcctggtca attccctggc taccgctgcc aaccgggctc gcaacgcttc gggacgcacc
    23401 actgtggcgt ggccccccat ccccagcacc caccgttccg cctcctccag gctgtccaac
    23461 cgcagccgca gccgcagcat gcccccgggc atttcggtca gttcctggct cgaatgcagc
    23521 cgccgacccc gcacgtcatc cgccgcaaag gcgtccaatt ccaccaccac ctcgaagtcg
    23581 tcctgtccct tgaacagccc caggctgcct tgcaggtatt cattcaggtc gaacttcttt
    23641 gacacggtga agcgccggcc cgtgaatttt gccttgctca gccgcgccag cacgaaagtc
    23701 cgcatggcct tccgggttac gtcgaagccg aagaggcacc actggttatt cacgcacgcg
    23761 atatgatacg gatgcacgtg ccgttgctgg gccttcagtt ggccccggtt ccggtagctg
    23821 aaccgcaccg cccgccgctc gctgaccgcc tgcgtcagca gggcaaacac cgccagttcc
    23881 gcgtcgccgg gggcaaacgg ccggaacgag agcaccccgt ccaggctgcc cagggagtag
    23941 cgcgcgctct ggtccaactg gccggtcagc ttgcggaagg tggactccag catctgttgc
    24001 aggggcgtgc cgcggtactg cgcgatcgcc ttgctggcga tgaacagcgc gaacgtgtcc
    24061 gcctcggaga tcggcatttg cgggaaatgc ggcacggagc gggtgaagaa atagccgttc
    24121 cgcgccgggt caaactcgag gggcaggttc aggcgggtgc gcatgaaatc cacgtcccgc
    24181 ttgatggtgc ggacgctcat ttcgaactcc cgcgccagcg tgtggcagtt gggatattcc
    24241 ttgttttcga tgagctgatg aatccgcagc attcgctcaa tcgccggcca gctatacacc
    24301 gtggttcgtg gtttgcgcat gattcacttg tggcggcgcg ggccgcggcc cgcggttccc
    24361 gggcttcgcc gtccgcccgc ctcgcattta aagcgcccgg cgcccgggcc acaactcttt
    24421 ttgtaatcgg gggtgccatt ccctgtcacc cccgcggaga aagccccggt tggccggcct
    24481 tttccggggc tccggcccgc cgggtttccc gggggcggtt cggaacgcgc ggtttttttg
    24541 taagggggag tgtcattggt tgtcactccc ggtcggttat tgtgttcggc gccgggtgag
    24601 ttccacccgg catgaacgaa aggaaaaaca tggaaaacgc agtacagagc cggttgcagt
    24661 ccgttcagtt tgggaaggcg cagcgctatc agaacctcgc gattctgccg ctcatcgccc
    24721 cggccgacgg cgcctgccaa tatcgcacgc tcggggaagc cctcgccgcc tgggacctcg
    24781 ccatcacgga agtgtcggag gcgggttcgg tccccaacct gatggtcatc aaccgcgcca
    24841 cacagcccgt cctgcttttg gatggggagg agctcgcggg cgcccggcag aaccgggtcc
    24901 tcaatacgtc aatcctgatc aagggatgct cggaaacccg gattccggtc agttgcaccg
    24961 agcagggccg gtgggcctac accacgcggg cctttgctga atccgggaac attatggcct
    25021 tcaaagcccg ggcccgaaag acccgttccg ttcatgagtc gttggaatcc ctgggctctt
    25081 accagtccaa ccagagtgac gtctggaata gcattgacaa gttgtactcc aaggctggct
    25141 tccgctcggc caccgccgcc atgagcgatc ttttcaaggc gcaaaccgag gcgcttctga
    25201 agtgcgacga ggtcttccag cttgttccgg gtcaggttgg gttgctggcc tttgttgacg
    25261 gccagcttgt gggcgccgag atgatctcgc gcagcgcggc ttatgcccgg cttcatgcca
    25321 agctggtccg cagctacgcg ctggaagccc tgatgccctc cgaccccgcg cccgcgggaa
    25381 agcccgggga gcccgccccc gcgcccggcg ggagcagcgc cccgcccaaa cccgcgggag
    25441 aggccgatct gaccgcgagc gcccggcagt tcttcgcgga agttgccgcc gcgaaagaac
    25501 agcagtttcc ctcggtcggg ctcggcaccg actgccgcta cgaagcccgg aacctggtcg
    25561 ggacggcgct ggtgcatgaa caggaagcca tccacgccgc cttcttccgt ctggatgact
    25621 ccgagtctcc ctcacatcgc cgccggcctt tcccgcgccg gcatccgctg ggatgaggac
    25681 gcctccggaa tgaacgggtt gcgcgcgggc ggcggccgga ttgcccggga atggaagcgc
    25741 aaactcctcc gcggcgaggt tcgaggtttg ccttccattt tccgggccgg gttctcccgg
    25801 cccggcgggc cgcggcggcg ccggcccgcg cgccttggcc gggggagccg gccccgggac
    25861 ggggggtgca ggtattgtct tttcaggagg ggggtgggta gggtagattt ccgggcgttg
    25921 ggctgggagc ccgcggaaga attatggcca aatataaaat tgcatggttg ccgggcgatg
    25981 gcatcggcgt tgaagtactg gaggcggcga agatcgttct cgatcggctg cagctcgatg
    26041 cggagtattt acccggcgat atcggctggg agttctggcg ccgggagggc gatgcgctgc
    26101 cgccccggac ggtggagttg ctccagcggg tggatgcggc catgtttggg gcgatcacgt
    26161 ccaagcccgc ccgcacggcg gcggcggagc tggcgcccga gctgcaggat cgcggcctga
    26221 gctaccggtc gcccattgtg cggatgcggc agttgtttga tctctatgtt tgcctgcgcc
    26281 cctgcaaggg gtatcccggc aatccgctca acttcaagga gggcatagac ctggtggtgt
    26341 tccgcgagaa caccgaggat ttgtatgccg gggtcgagtt cgcgcccgtg ccgccggagc
    26401 tggcccgcgc gctcgggcag cttgcccggc cctttgcgcc gttcgcgaag ctgccgcgcg
    26461 accaatacgc gatctcctgc aagatcaata cgcgggccgg ctcggagcgc atcgtgcgcg
    26521 ccgcgttcga tttcgcccgc aagcacgggc gcaagaaggt caccgtggtg cacaaggcca
    26581 acgtcgtgcg ggccaccgac ggcctgttcc tcgaagccgc caaagccgtg gcgaaggact
    26641 acccggaaat cccgatggac gacgccaaca ttgacgccat gaccatgtgg ctgctcaaga
    26701 acccccacaa ctacgacgtg ctggtggcgc cgaatcttta cggggatatc atttccgacc
    26761 tctgcgccca gatggtgggc ggcctgggat ttgggtgctc gggcaacctg ggcacccggc
    26821 tggcggtgtt cgagccgacg catggctcgg cgccgaaata cgccggccaa tacaaggtca
    26881 accccatcgc caccatcctg gcggtcaaga tgatgctgga ttggctgggc gaggcggcga
    26941 aagcccaggc cctggagcag gcggtggccc gggtcatcca ggacggccgg gtgcgcacct
    27001 acgacctggg aggaacgagc accaccttgc agatggcgca ggccatcgcg gaacagctat
    27061 gagcgccgcc gcttccgggc cggagacccg ccgggttttg ctccacgaag tcgggctgcg
    27121 cgacggcctg cagatggaga agcaggtggt tcccctcgag cagaaggtgc gctgggcgga
    27181 agccctgctg gaggcggggg tggatgtggt gcagttgggc tcgtttgtgc atccgggcaa
    27241 agtgccgcag atggcggaca ccgacgcgct ctttcagcat ttcaccgcgc ccgggcgcaa
    27301 accggccggc gcgacgctgt cggggctggt gctgaacgag aaggggctgg agcgcggcct
    27361 ggcgtgcggg gtcgagttgt tttgcatggg ggtttcggcc agtgaaaccc acagccagaa
    27421 gaacaccggc atgagcgtgg cggaggccac cacgcgcatc atcgccatgg cgcaacaggc
    27481 ggcgggcgcg ggcaaaaagg ttcagctctc cgtccagtcc gcgtttggct gcggatttga
    27541 aggcccggtg ccggcggagc gcgtgctggg catcgccggg cggtttctgg aggccgggtt
    27601 tcggaacctg agcctggcgg acaccgccgg gcaggggacg ccggagcagg tcgagcagtt
    27661 gttcggcgcg atcttacaac gggcgccgga cgccgaatgc accgcgcatt tccacaacac
    27721 ctacgggctg gggatggcca actgttgcgc cgcgctgcgc gtgggggtga agtgtttcga
    27781 gtccgccgtg gccgggctgg gcggctgccc gttcacgaag gtggccggcg ggaacgtttg
    27841 caccgaagac ttggtgtatt acctgcagcg gcggggcctg cggccggacg tgaaccgggc
    27901 gcggctggtg gaagtggcgc gcgacctgag ccggtttttc gcgcgcgacc tgccgggcat
    27961 gatttacaag atatgacgga ttcaactcat tcaaccgcgc ccccgcgcgc ggcgctggaa
    28021 ggcatccggg tgctggacct gaccaacgtg ctggcggggc cgtttgccac gctgcacctg
    28081 gcgttgctgg gcgccgaggt catcaagatc gaaaacccgg cggatgggga cctggcccgc
    28141 aagctgggca acgtgccgcg gctgaaccag gagctgatgg gaaccagctt cctggcgcag
    28201 aactccaaca agaagtccct gaccctgaac ctgaaagcgc cggccgggcg cgagatcttc
    28261 aagaagctgg tggcgaccgc cgatgtgctg gtggagaact tccggccgga tgtcatgaag
    28321 cgcctgggac tggattaccc cgtgctggcg cagatcaatc cgcgcctggt ctattgcgcg
    28381 atttcgggtt ttgggcagag cggccccgat gccttcaagc cggcctatga ccagatcatc
    28441 cagggcctga gcggcgtcat ggcgatcaat ggcgacgagc ggctcaaccc gctgcgcgcc
    28501 gggttcccgg tgtgcgacac cgtggggggg cttaacgccg cctttgcgac gctggcggcg
    28561 ctgttccacc gccagcggac cggagagggg cagttcattg acgtggcgct gctggattcc
    28621 atcatgccgc tcatgggctg ggtggcggcc aacctgttga tcggaaagca gcagccggtt
    28681 ttgatgggca acgacaattt caccgccgcg ccctcgggca ctttcgtcac ccgagacggg
    28741 tttattaaca tcgcggccaa caagcaggag cagtgggagg cggtggccga cctgctcgga
    28801 gtgccggagc tgaagaccga tccgcgcttc caggagcgcg accagcgcaa gaagaaccgc
    28861 aaggcgctca cgcctctgct cgaagcgaag ctgaaggaga aagacaccgc ctattgggtc
    28921 gagttgttga accgcaatga cgttccctcc ggcgcgattc tcagcctcga gcacgccctc
    28981 agccagccgc aggtgcagca tcgccaaacc ctgcagccgg tcgccgtcga aggcatcggc
    29041 cagctccagc tcttcggcct gagcgcgaag ctggagaaaa cccccggcgg aatcaaaacg
    29101 ccgcccccgc gtctttcggc ccacacgaac gccctcctga ccgaactcgg gtattcgccg
    29161 gccgacctcg accgcttcaa ggcggagcgc gtcatttaag gccgcccctg ctgattcctg
    29221 gactcccttt aacccgtaga tacccttgct gaacttatga ccgtagttga aaagatcctg
    29281 gcccgcgcca ccggccggcc ttcggtgaag gctggcgacg tcctcgaacc caaggttgac
    29341 cttgccatgt cgcacgagaa tgcggctttg gtgatcaatc agtttcgcga aatctaccag
    29401 ggcacgccgc tgccgccccg gatttgggat gcccgccgcg tggcggtgat ctttgaccat
    29461 cgcgtcccgg ccgaatcgcc caagacggcc tccaaccaga agaaggtgcg cgagttcatc
    29521 ggggcgcagg gggtgggcaa gttccacgac atccggggcg atgtgggcgg catctgccac
    29581 caggtcctgc cggaaaacgg ctatgtccgc ccgggctacg tggtggtggg cactgattcg
    29641 cacaccacca gccacggggc gctgggggcg tttgccttcg gcatcggcgc caccgagatg
    29701 gccagcgtct gggccctggg cacggttctg aacgtcgaag tgccgccgac catcaaggtg
    29761 atcgtgaagg gccgcttccg gaagtttgtg ggcgccaagg atttgattct gcacattatc
    29821 ggccggatca gcgcccaggg cgcgaatttc aaggtgctgg agttccacgg cgaaaccatc
    29881 cgccgcctgc cgacctccgg ccggctggtg ctctgcaata tgtccgtcga ggcgggcgcc
    29941 acggcgggca tcgttccggc ggacgaagag acggcgcgtt acttgcgcga ggaggcgggc
    30001 gtgaccgatc cgctcgagcc ggtgctgccg gatgccgggg cgaactatga aagcgtgctg
    30061 gaggtcaatg tttcccggct ggagccgcag atcgcctgtc cgcataccgt ggataacgtc
    30121 aagccgattg gccaggtggc cggcacccgg gttaaccaga ttgtgattgg ctcgtgcacc
    30181 aacgggcggc tcgacgacct ggctgtggcg gcgaagattc tcaagggccg gaaggtggcg
    30241 ccgggcacgc gcatgttgat tttcccggcc tcggcgaaaa tcttccgcca ggcgctgggc
    30301 aaaggctata tcggcgtctt tatgcaggcc ggcgccgtgg tgatgaactc cggctgcggc
    30361 ccgtgcctgg gcattcacca gggcgcgctc ggcgatggcg aaaccgcgct ttccaccacc
    30421 aaccgcaatt tcaaaggccg catgggcaat ccccaggccg cggtttacct gtgttccccg
    30481 gcggtggccg ccgcctccgc cctcaccggc gtcattaccg atccccgaaa atcctagcaa
    30541 cccgcgcttc gtgcgcctcc tttgatctat gccaaatgtt gtcttcaaag tcggtgacga
    30601 tgtttccacc gacgtcatct acccgggccg ctacatggcc acggtgctgc ccaccgagac
    30661 gccgcagttt gcgtttgcgg atgagacggc tttcaacgcg aagctcaaag ccaggcaggt
    30721 tcctccgggc agcgtcctgg tcggcggccg gaactttggc tgcggctcct cgcgcgagca
    30781 ggcggtgtcc tgcctgaagg ggcatgaatt gatcatcgtc gcccggagct ttgcgcgcat
    30841 cttcctgcag aacgccatta acctcggcct gcgcatgatt gtttgccccg aaattgaggc
    30901 cgccgagggc gacgagttgg agctgaccgc cggggccgtg gtgaacaaaa ccaccgggcg
    30961 gcagttcagc gtcgagccga tgccccagtc ccggcaggtc attattgacg ccggcgggct
    31021 gattccctac acgcgcaagc ggttgctggc gcaggcggcg gcctgacccc gggcggcgcg
    31081 ggttcccgcc gcgttccggc ggcctttact tgggcggccg gacgagccat tttatcctta
    31141 tgcgattgct tgccgggcta ctgtcgggct gcgccgccgt gttcgtagcg ggaacgctct
    31201 gggcgggagg cagcgggttt aacgtcatcg tcgtcgccaa ccaaagcagc accaactcgg
    31261 tgcggctcgc caatcgttac tgcgagctgc ggggcgttcc gccgcagaac ctctggcgga
    31321 tggaaggctg gaccgcgcgg accaatacgt ggagccgcgc caacttccag gcgtttctgt
    31381 atgatccctt gctggcggcc atcgccgccc gcgggctgac caaccaggcg gagttcattc
    31441 ttctctccat ggacatcccc taccgggtgc tggataacga cccctaccaa acccagaaca
    31501 gcaccaccgc ggccctgttc tacgggtgca agcgcgatac cgcgccgccg ctgatctgtt
    31561 tgcccgcctg ctgcggcctt cccgccgcct cgtccaacag ctatgctttc agcgaaatgc
    31621 cgttccgcga agccccgccc gacaccgcgc tcaccaatgc cttcctgacc ctgatgctga
    31681 ccgccagcaa cctggcgtcc gcggaacgca tcctcagccg cggggtggcc agcgacagca
    31741 ccttccccac ccaggccgtg tacctggcga aaagcagcga tccggcccgc agtgtccgcg
    31801 ttgtcaaatt cgacaacgcc atctttgaca gccgcgtccg gggcgactat cccctggtac
    31861 gcaccaacac cggttccacc gcgttcacca atctcctcgg gttgcagacg gggaagacga
    31921 gctttaccct gccgaccaac gcgttcgtgg ccggggccat ggccgacaat ctcacctcca
    31981 atggtgggaa aatcttcgag gaccgcgccg gccagaccgc gttgctggcc atgctcgacg
    32041 cgggagccgc cgggagctat ggcaccgtcg tggagccgtg cgcctacacg cagaagtttc
    32101 ccgatccgcg caattacgtt taccagaaac gcggcttttg cctcgccgag gcctattacc
    32161 aaagcctgct gaatccctac caggggctgc tggtgggtga gccgctcgcg gcgccgtttg
    32221 cgcgccgcgg cacggcggac tggaactcgc tgaccaacgg cgccgtgctc agcggccttg
    32281 cgctgctcac gctggatttc accgccgccg ataccaacct gccgctcagc caggttgatc
    32341 tttttgtgga cggccgcttc tggcagcgca tggccgacct gcccccggcg gcgggcaatg
    32401 agctggcggt gaccctcaat gggatcccct ttaactatac cgtgcccacc aatgccaccg
    32461 tcgcgtcggt cgcggccgat ctggccgccg gattgaatgg cctgacgaac ctgacgcacg
    32521 tgcaggcctg ggccgtgggc gaccgcctgg aattgcaatc cctcgaagtg gcgcagccgg
    32581 gcggtgccgt caccctcagc gccagcaccg cggccggttc cgccgcgcaa ctgaccacat
    32641 ggctgacccc ggcccgcgat accttcctgg acaccaccgc caccggcggc ttgagcctgc
    32701 tggccagcaa cacgcccctg gccggggact ggctgcagct cgacttcatc aagaccaatg
    32761 gcgagtgcct cagcctcagc gtgaccaatc cggccgcggg caccgatatc gccacgctgg
    32821 tctcgaacct ggtcaaccgg gttaaagccg agcccgcgct gcaatccgcc gatggcgtgc
    32881 tcgcggccga ctttgccgac gacacctact gcggcatcgt ggcggcggag ttcaccctgt
    32941 atgcccgctc gcccggctgg ccggccgcgc aattccaggt ggcgttctcc gcctcgacca
    33001 atctgctggc cctgccggcc ggcaccaatt gcctggacgc caacctcaac gacctccgcc
    33061 cgcgcaacca tctgtatgtc agcagtggcg ccgccgcgct gccggtgagc tttgccttcg
    33121 acaccacccg ggtgccggac ggctggcatc aactcaccgc ggtggcttac gagggcacca
    33181 gcgtccgcac gcaaacccgc gtcgcgcgga cggtgcgggt ccagaacacg gcactggcgg
    33241 ccacgttcac cccgctgctg gccggcacga atgcgacgct tgatctgccg ctgcagtttt
    33301 ccgtggcggc caacgccacc aatatcgcgc gcctcgaact cttcagcacc ggggggacgg
    33361 tcggcgtggt gtccaaccaa tccggagccg tcttcaccgc gcccgtggcg gccctgggcc
    33421 tggggttgca tcctttctat gccgtggtga ccgatgccga cggaaaccaa taccaaacag
    33481 agaccatttc catccgcctg gtgcctccct tccggctgcg cctggaccgg gcgccgttga
    33541 cgctctcctg ggaggccacc ccgggggtgc cgtatgatgt attggccgcc accaatctcg
    33601 cccacggatt cgcgccggtc gcctcgctga cggcttcgaa catcctggcc gtgtggccgg
    33661 tgccggttcc gggcgaagcc gcggcgttct accgggttcg gctcagccct tgagcccgcc
    33721 gccgtgcgcg ccccgcccga cgcgcgggaa ctaattgtct ttccctgcgg ccgcgcttcg
    33781 gaataaaatc cgtttatgcc tgagattgtg gcttttgtta acagcggggc ggcgacgatt
    33841 acgccggcga ttgtggagcg gatgctgcgg cagcttccgc aatggaaact ggagtttacc
    33901 cagatccacg aaccgttgtt tccccacctg gtggatcagc tcgagttcct ggctgacgtg
    33961 gtcgaagatg tcgcggaagg agtctataaa gatctgccgt atgccgcttt ctgccaggcg
    34021 gcctttgcgc tgatttatgc gcataagaaa gtgggcatca tcccggacac ggtgctgaac
    34081 ctgggacgcg cggacgactc cagcgtggtg cgggccgtgc tgattcagaa cgaaagcgct
    34141 tttgcccttt acgccgccgc gcaaaacatc gagtggtccc gcatcaccag ccagccgtag
    34201 ggccgcgccc gtttcgttat ttggcggccg cggtctcccg cgccgcgcag caactggtct
    34261 tgaggtaatc ccggaactgc tggcgctggt gaaccacctc gttatgcggc aggtcgctgt
    34321 agaggaagat taaccggttg gcgcggaggt cggccagccc ggcggcgctt tccttggcgg
    34381 cgtagaaggg cgtgtgctgt tcccgcaagc gcgccattaa acgctcgctc tcggccttgg
    34441 tgccgccctt ttcggtgtaa atgtcaatga accgccccgc cgagcggttg atccaggcca
    34501 ggaaccggtc cgtctgcgcg tagagggcgt cgaacagcca gacctcgcgc aggtgctctt
    34561 ccagtccgcc ctgggccacg atcgccgaga tcacctgata gccgccgctg tggcccgaaa
    34621 gcaggatttg gccgatcgcg aatgatttgt tggtcaacgc cgatttctgg cgcagcgtgt
    34681 cggccacctc cgccatgaac cgctggaagc cgccgggatc ctccagcttc ccgccaaagg
    34741 aatccggggc gttgcgcggg ccctggggaa cgaccagcac cgcgttgcgc tggctttcga
    34801 tgagttgctc gatgagctga tagcgggtca ggaccccttc cacctggttc ttccagccgt
    34861 ggaagtggac gatgaaatca attttgcccg tctcgcggaa gccttggggg ataaagatgg
    34921 ccaccgtatt gtcagaataa tgctcccggg cgggaaagaa ctgctctttg tagcggtggc
    34981 cctcggcgcg ccggggatgc ggaaacggcg cggcagccag cggggccagg atcagttcgc
    35041 cctggctggc gtagcgttga ggaagagttt cgccgcccgc aatcaaggtg ctgcagccca
    35101 accaaaatgc ccataaccgg ttcatgagtt ccgggacagg atgcccgatt gccggcgctg
    35161 gcaaaagcag attatgcggg gcgcgggccg ccgcttatcc ggaagccggg ttccgcgggc
    35221 cggggggccg ggcgcgcccg ccggattttg cgccggtctc cgtgccaaaa ctggacaccc
    35281 gtgtctgacg attggaaccc gctcgcccgg gcgcggggaa aatctgccgg agtagggccg
    35341 ttttggcaaa ctggccgcaa gtttgcttca aaggagccgt ttgacgcgga aatgaatcaa
    35401 cgcgtagcag ccccgaacgg gtaacagcag aattcgcatg gccaccacat taagttcgag
    35461 tgaacgggct caactggcgc agaccgttga gatgtttgag ggcattacgc aggcggaacc
    35521 ccacgattac caatcgctgg agattctcaa agaagcgtat tccaagctca accaggaaaa
    35581 ggatgtcatc aacacgtcca agcgcattgc ccaggcctat gtgcaactgg gccagttcgc
    35641 ttcggcaaag ctcgaatatg aggccatcct gcagcgctgt ccggacgatg cggaagccaa
    35701 aacggctttg caggaaattg ccagccaggc ggacaacctt cccgcggagc cggccgcgcc
    35761 cctcgaaatc gcgcccgccg ccttgcgggt ttccgcggcg cccgccccgg ccgcccccgc
    35821 ggcgggccaa gcggcgcccg gcgacgtcga ggacgggcgg caaacgatgt ataaactgtt
    35881 tgtcgaaagc cggatcatcg cccccgccga tttcgggctg tgctggccgg tggtggatcc
    35941 gcgcgtcgcg cccgagggcg tcatcgagcc ctttatccag gcgctggccg accgcaacat
    36001 cctgccggcg gaccagtcgc tgaaaatcct gtccgaccag tcgcgctgcg cgtttatccc
    36061 gctcaacacc tacgacattg acatggatct cgcgcgccgg ttttccgcgg cgacgtgccg
    36121 ccgctggtgc gtgctgccct tcgaccggat gagcaagtcg gtgctggtgg ccaccgccaa
    36181 ccccttcaac caccaggcgg gcaaagaact gagcaaggcc gccggcagcc ggctgctgtg
    36241 gtacctggtg ccgccgcacg aactcattat caatctccgc aaagccttcc gctaaaccca
    36301 tgcctccggt caaatcattt ggcgaacgca tcgttgacgt cctggtcgaa gacagcttgc
    36361 tcacgagcaa gcaggtcgcg gaaatactcg aactgcagaa gaaggaaggc acccgcctgc
    36421 tcgagctgct gttgaaaaaa tcctacgtca ccgatctgga catggcggtg gcgatggggc
    36481 gcgtgctcaa catcccgccc atcaacctgg cgcacctcaa catccccctg gaagtggcca
    36541 acctccttcc gcgggaaatc gcccacaacc ataaggtggt gcccgtctcg cggctggaaa
    36601 acaagctgtt cctggccatg gccaacccgc tcaacgtcct ggcccgcgac gacgtgcgcg
    36661 acatcacccg gatggaagtg gccatgctga tcgccccgga aaaggcgatc caggacaagc
    36721 tggcccaaat tgacgccgtc cggggcggca gcatcgagga catcatccag gacgcgcaga
    36781 aaaaggccat tgccgacgag gacgcggaca acgtggaggt gtcccgggaa gcgatcgagg
    36841 aggtcaacct ggacgaactg gctgcgtcca ccgaagaggc gcccgtcatc aagctggcca
    36901 acctcatcct ggtccaggca gtgaaggacc gcgccagcga cgtgcatatc gagccgttcg
    36961 acaaaacgct gcggctgcgc taccggattg acggggtatt ggtggacgcc accccgccgc
    37021 ccaaacaaat gcaactggcc ctggtctccc gcttcaaaat catgagcagc ctggacatcg
    37081 cggaacgccg ccagccgcag gacggccgca tgcgcatccg cgtgggcggc aaggactacg
    37141 acctgcgcgt ttccatcatg cccacggtgc acggggaaaa ggtggtgctc cgccttctgg
    37201 acaaggccaa cctgtccgcc agcatagaca aactggggct ggacccggat acgttccagc
    37261 agttcaagac ggccattgat gccccgcacg ggttgatcct ggtcaccggc ccgacggggt
    37321 ccggcaaaac cacgaccctg tattccgcgc tcaacgaact caacgacccg gtttacaata
    37381 tcgtgaccgt agaggacccg gtcgagttcc agattcccgg catcaaccag gtgccgacca
    37441 aaaaggaaat cgggctgacc tttgccaacg tgctccgctc gatcctccgc caggaccccg
    37501 acatcatcat gatcggtgaa atccgggaca cggagaccgc cgaaatcgcc atcgaagcgg
    37561 ccctgaccgg ccaccaggtc ctgagcacga tgcactgcaa tgacgccccc ggcgccatca
    37621 cccggctcga cgacatgggc attgcgccgt tcctgatttc ctcgtccgtc atcctcgcct
    37681 gcgcccagcg cctgatgcgc cgcatttgct cccactgcaa agagccggtc acttacccgg
    37741 ccaagatgta tcaggacctg aatatagacc ccagcatctt tgacggggtc acgctctacc
    37801 gggggcgggg atgcgagcgc tgcaagaatt ccggctacgc cggccgcatg gccatcattg
    37861 aggccatgac catcagcgac gaaatccgca agctcatcat cgcccgcgcg aactcgcgcg
    37921 agatcggcaa ggtcgccatc ggccagggca tgcgcacgct gcgcatggtg gcgctggacc
    37981 gcgcccagga gggtatttcc accctggagc aggtgctcgt gctgacctcc acccattgac
    38041 ccgccgcccc gcgcccggcg cgccctccgg cgtttcccgg gccccggccg cttaccgggc
    38101 cagcccggca atctgccgtt ccgtcagcgc ggcgtcgtac aggcgcaagt cccggaggct
    38161 gccgcggaaa taatcgttcg ggccgaaccc gattttcagc ggctgcgcca gcgcaaggtc
    38221 atacggggcg gagtcgaacc gggccgagac cgccacgcgt cggccgtcca ggtaaagcgc
    38281 gagccggccg ccgccccgcc gggccgccag gtgatgccag ccgggggaaa gcgcgcggtc
    38341 atgcgtcacg ttgcgcccgg cttcgaggga aaaaacccgg ccgccgggca gcgtgccgca
    38401 aaagagccgg ccctgaaact cgctcatcgt ccacgcgcgg cggtaggtga ccttcggcgt
    38461 ttgatcgagc ctgccaaccg ggcgccagtt tcccgggcgg tcgagccgat aaacctcggc
    38521 cagcggcagg ctgccggcgt agagcatccc gttatgcacc agcatcccca tgacttcgag
    38581 ttcgccgccc agccgaccca ggtcctccca gttatggtcg ccgcgatacc cgtacacccg
    38641 ccccgtcttc cacgtcccca cacagaggcg gccttcgtaa atggcgaaag agtaagtttg
    38701 cgtgttttcc agcggcccca cctggccgca atccgtccag ccggttccgt cgaaacggaa
    38761 tacatgcgcc tcatcgtagc tcgacgcgaa aagctggccg tcatacacgc acagggcgag
    38821 cacgcgcctg ccgggcgtgg gcagcgcggt ccaacgccgc ccgccctcga aacggaaaaa
    38881 ccccgccggc ggataaagcg ccgaggcgta aagctgcccg cgataaacca ccagcccgcc
    38941 caccgtctcg ctgccgggca acgggccgca gtcctcccag gcctggccgc cccggtagcg
    39001 aaagactttc cccccggcgt tggtattcgg ggattccggc agcgccgagc cggccaggcg
    39061 ataccgcgag accccggcgt agagcgcgcc ctggaactcc gccagcgcgg agacggcatt
    39121 gcacggggcc ggggggccgc aatccaccca gttcgtgccc ccgccaaacc gatacacgcg
    39181 cccggcccgc cccggggccg gttcgcaggt ggcggcatag agttcgccgt catgcgtggc
    39241 cagtccgaaa atcaggacgg cctcgcccgg ccgcccgtgg tcggtccagt gcggctccag
    39301 ccgcccgtca tcaatgccaa agtgaaccgt gcgcgcgttc gcctggctcg tagtcacgcc
    39361 cgcattcagc gaaatgcgga aattgaatcc gcgccgggtc gccgggtcaa accggctcac
    39421 aatgtcccca aacacgtcgt cctgctccgc ttccgccggc aggcgcaccc aggcggcgag
    39481 ggtgaagtcg ccggttccca gccgcagcga cggcgacggc ggcacctcca gccagctttg
    39541 ccgcccgtcg aactgcgccg acttccggtg ctccgccgtg gcaaagcgca ccgcgtgcgg
    39601 gattccgtgg ttgccgttcc cggaagcatc acggccgtcc ttcgttaatt tccagtgcgc
    39661 gagcagccgg gccttgcggg ccgccggggc ccggccaggc accccttctc ctcccgcgcc
    39721 cgggggcggc gcagatcggg cgggcgggga cggcatagct gccaggagca gcgcgagcgg
    39781 gagcagcagc gatcgtttca tgccgcggct aagtttgcgg cggcgcccgc cggctgtcca
    39841 gttcgcaaag gacggcgccg gcggacgcgc cgcgccgttc ggagggagcc gccccgccgc
    39901 ccgccccggc ccgccggagc ggtgagccgc ccgggctttc gttaacgatc gggggctttt
    39961 gccttttgtc ctccggccgg ttgtggtttt ctgccgccgt gagcccgcga gtattctgca
    40021 aaacccggcc cggcgcgtcc ggccgaagag tctttcaccc actggcggaa acctccgcgc
    40081 cccggcgaaa aatcgtttgg cgcggcctcg cgttcctcct cgccgcactg acgcccgccg
    40141 tcggcgcggc gccggtcatg cgcacgatga agaccgccag tgccggagtc ccgctgctgg
    40201 ccaacggcgg ctttgaggaa actcacgcgg gcaggccggt ttcctggacg gcctggcggc
    40261 agggcggccg cctcgcgccg ggcgagggac gccagggcac gcacggcgtc atgtgcgaac
    40321 gccgggacgg cggcggcgaa tatggcttca gccagacgct cacgctcaac cggaccaacg
    40381 tcgcgccctt catcattcgc ggctggagca aggccgagca ggtcagcggc agccccgacg
    40441 gcggctacgc gctctacgtg gacattctct acacggacgg caccccgctc tggggccaga
    40501 cggccagctt caactgcggc acgcatgatt gggagcagcg ccagtttatt ctgctgccgg
    40561 ataaaccggt ccggtcgctg accttgcact gcctctttcg cgggcacaca ggcaaggtct
    40621 ggttcgatga tgccgccgtc gaggaagtgg ccgcccccgc gggcgcgttt ctgtttcaag
    40681 gcgtgccgct ccagccaagc ccgcgggaag aaccgccccg ccgggccgcc cccacccggc
    40741 tcgccacccg cgacgggctg aaactgatcc tgcgcggcga cgcgtccgcg tccctgcggg
    40801 tcgggcgaac ggaactcggg agccacgcgc ccgcgggctt cctggtccgc gacttcgccg
    40861 cgggttcgga cttctacggc tttgcggcgg agggaagctg cgccgaactg gggctgcggc
    40921 tggcggcgga cttccacgcg ggcgcggatc atatcgcggt gagcgggcgc ctcaccgaca
    40981 ccaccggccg cgaccgcgcg gtgaccttgc tctttgccct gccgctggac gccaccggct
    41041 ggcactgggg cgacgacgcg cgccagagcc ggcgcattga aggcggcggc gaattcgcca
    41101 ataccgtcaa cctgcggagc ggggccaacg gcaaaatgtc gctctatccc ctggcggcgg
    41161 tgtggaacga acgcgccggg ctggcgctgg ggctggacat ggatcacccc gcccaatacc
    41221 ggctggtcta tcatgccggc acccggcaat tctttatcgc ctacgacttc ggcctggtcc
    41281 cggagaccgc ccggtttccc ggcggcgccg atttccggtt cgtgatctac cactttgacc
    41341 cgcgctgggg ctttcgcgcg gcgctgcaaa agtattacga cctcttcccc cggcacttcg
    41401 tccgccgcgt ccagcgcgaa ggcctttgga tgccgtttac cgacatcgcc cgcgtgccca
    41461 accccgcgga tttcggcttc gccttccagg agggcgcgcc caatgtcgcg ttcgacgacc
    41521 ggcacggcat tgccagcttc gtctatgtcg agccgatgag ctactggctg gcgctgcccg
    41581 ccgacgcgcc ccgcacctac gccagcgccc tgtcggtcct gaccaacgac ctgcgcggcg
    41641 cccggggcgc ggaaaaggct gaaatggccg ccgccacgct caccagcggc atccgggatg
    41701 ccgccgatca gttcgcgctt tacctcgtca aagctccctg gtgcgacggc ggggtcttca
    41761 cgctcaaccc ggacccggcc attccggcgt cgcccgaata tcccttcacc aaggcggggg
    41821 tcatgcaccg ccaaatcgag gccgctttcc gccggcatca gcccgccgcc accaacacca
    41881 ccccgggggc cggcctggac ggcgtctatc tggattcgct ggagatgtcc agcgccgagc
    41941 tgaactaccg ccgtgaacat ttccgcaccg cccaggcgcc gctggtgttc gaccgggaag
    42001 gccggccctg ccaactgatg atcttcaaca cctggacctt cgcgcgcgaa cttgcccgcc
    42061 aaatgcacga ccgcggcaag ctggtcttcg ccaactccgt gctctggcgg ttcgccttcc
    42121 ccgccccgct cctggatgtg ctcggcacgg aggtcaactg gctctcccag ggcgagtatc
    42181 atccggacac ggacgcggtg atgaacttcc gccgcgccct ttgccgccaa aagccctact
    42241 gcctgctgct caacaccgac tatgcaaagt tcactcccga ccgggtcgaa cgctttttcc
    42301 agcgctgctt gttttacggt gtgtggcccg gattcttcga cccggaagcg gcctccaaag
    42361 acccctattg ggcctccggc aataagtggt acgaacgcga ccgcccgctg tttaagaaat
    42421 acatcccctg gttccgccag ctcaccgccg ccggctggca ggccatcccc catgccacct
    42481 gcgataaccc ccggatccaa atcgaacgct tcggcccgga tcccggcggc gccgtatggc
    42541 tgacgctgtt taacgacacg ccccatcccc agaccggggt gatcaccgcc gatctgaagg
    42601 cgctgggcct gaagcggcac ccccgtgcgc gaatgttgct ttccggcacc cccgccaaac
    42661 cccagcccgg cggctggcag gtctccctct cttcccagca ggccgaaatc ctgtgcctgg
    42721 cctccgaatc ccgccatttc cagggccaaa cggactaaaa cctctgattt gctaaccgcc
    42781 gcccttggag tcacttatgg ctaccatccc gctgtgtaca aggggtgtac aaggggtgct
    42841 caagcggtgc ccggcagagg tacaaccaaa aaccctatgt acccccggtg agcatccctt
    42901 gtacacccct tgtacacggc tctttgagag cgggaaatgg gctgtttggg cggggaaacc
    42961 cgggggcggg agggggaacc ctgcgggggg ctttggtttc ggggcgttgg ggaatcaggg
    43021 ggtgtgcgtc cgttcgcccg ccggggttgg ggttgccgaa agcgggcggt tggtggggtt
    43081 ggggtgccgg ttcggggtgg aaagggcgcg gcatggggcg gttcgcatga ttgacttggc
    43141 aggggggtct gttagccttg ggacgtccgc atggcaagaa aggcttccag atttcggcag
    43201 atttgcgagg acgcgcgggc gctgttggac gagcgggcac cccgggcgct gactgacttc
    43261 tcgaagtggc agcggtttgc gcacttctgg attctggtgg gccggagttt tgtccggaac
    43321 cgctgtccgg tgcgcgcgtc ggcgctgtcg tacgtgacgg tgctggcgct gattccgatg
    43381 ctggccgtgg tcatgagcat ctccagcgcc ctgctcaagc aggaagggga ggagcagata
    43441 gaccggttta ttgatcagtt tgtgacccgc atcgtgccgc cggccacatt ccacaccaac
    43501 cgggaaagcg cgtctccgca ggcccttaat ccgccggcgg cgaccaacct ggcggtggtt
    43561 gcgccggacg gttcgccccc ggcggtcaac agcctggttc cggccgaccg cggtggcgag
    43621 acgaattcga gcctgctgcc ctccctgatt caggatgatc gggcggtcaa ggcccggcgc
    43681 gcggtggcgc ggaatatcca tgatttcatt caaaacaccc gcagcggcac gctggggatt
    43741 acgggcagca tcctgctgat cttcatggcg atttcgatgc tcagccggat agaagacacg
    43801 tttaacgata tttggggggt ggcgcgcggg cgaagctggt tcacgcgcat cgtgctgtat
    43861 tggggcgtga tcaccctggt gcccctgctg ctgatcgtcg cgctggggct ggcgacgggg
    43921 ccgcatttgg cgggcacgcg ggagtttttg acggcgatgc cggtggtaag caaactgact
    43981 ttccagttcc tgccggtggt ggttttatgc ctcagtttca ccttgtttta cgcgcttatg
    44041 cccaacaccc gggttcgctg gccggcggcg ctggcgggcg gtttgacggg cgggcttctg
    44101 tttcatttga ataatttgat cagcgtgctc tatgtctcgc gcatggtgac caccagcaag
    44161 atttatggca gcctggggct ggtgccggtg tttatgatcg gcatgtattt ctcctggctg
    44221 attcttttat ttggcgggca ggtggcctat gcctggcaaa accgggccac ctatctcgac
    44281 gaacggcagg cggagaacat caaccagcgc gggcgcgagt ttgtggcgct gcggttgatg
    44341 acctgcatcg gcgaacggtt cacgcaaggc gcgccgccgc ccaatgccct ggagatagcc
    44401 gaggggctgg cggtgccgac ccgcctggtc aagcgcatca cgcagaccct cgccgccgcg
    44461 cggctggtga cggagacggc gggggccgag ccggcgtatc tcccggcgcg cccgctggac
    44521 cagatcacct gccatgatgt tctcctggcc atgcgcgcca gccagggaca ggaatgcgcc
    44581 acgcgggacg agccggcgcg ggcggaggtt tacggcgagt ttcaccggat cggagaagcc
    44641 gagcggcagg cggccacggc ggtgagcttg caggcgctgg tcaaccgggc gcagcggttg
    44701 aagcaactgc ccggcccggc ccgcccggcg gccggggaat aggcattggc cggcggatgg
    44761 cccggccggg gcggggggcc ggagcgccgg attatcgaaa cgcccttgcc gccgggggtg
    44821 tttggcttta tggtagcctc gtgtgcgagc cgaacagagg cggggggata gcgaaaggca
    44881 tctgcctttg cctcccccgg gccggggccg ggtcgtcttt tgaaagctga cgctgtcgtt
    44941 tctgccgcca cgggcatttt ttatgattat tggcgttccg aaagaaatta aggatcacga
    45001 gtaccgggtg ggattgatcc cctcgggggc ttatcaactc atcaagcgcg ggcaccgggt
    45061 ggtggtggag cgcaacgcgg gggccggagc cggctactcg gacgccgagt atgttcaggc
    45121 gggcgcgacc ctggttaacg accatgcggc ggttttcgag caagccggcc tggtggtgaa
    45181 ggtcaaggaa ccgctccctt ccgaatacga gctgctccag cccgggcaac tcctttatac
    45241 ctaccttcac ctggccgcca gccaaccgct gacggaggcg ctgatgaagg cccggatcac
    45301 cggcctggct tatgagacca tcgaggtcaa tggccggctg cccctgctgg agcccatgag
    45361 cgagattgcc gggcgcatgt ccgtcctggt cggcggctat ttcctgggga agcataaaaa
    45421 cggcagcggg gtgctgctgg ggggcgtgcc gggggtgttg cccggcaagg tcgcggtgct
    45481 ggggggcggg acggccggca tcaacgcggc gcggcgggcc cagggcatcg gcgcggacgt
    45541 gaccattctg gaggtggacc tggagcggat gcggttcctg gacatcacgc ttaacaccgt
    45601 ccacacgctg tattcgaacg aggtcaacct gctcgagttg ctgccttcgg tggatttgct
    45661 gatcggcgcg gtgctggtgc cgggggccaa ggcgccccgg ttggtgcgcc gggacatgct
    45721 gcggcgcatg cggccgggca gcgtgctggt ggacattgcg attgaccagg gcgggtgcgc
    45781 cgaaacttcg cgccccacca cgcacgatca cccggtattt gtcgaggagg gcgtggcgca
    45841 ttattgcgtc gccaacatgc ccggggctta tgcccgcacg gcgacgcagg cgctgaccac
    45901 cgccacttac cgctacctgg agctgatcgc ggaccgcggg ctggcggagg cgtgccagtt
    45961 ggagccggga ttggtggggg ggatcaacgt gatggggggc aagctcaccc ataaggcggt
    46021 ggccgaggcg catgagctgg cctgctcgcc gcccgtgctc tgaggcgccc gccgccccgg
    46081 aggtttccgt ttttcattcc gataatgaag gccccaattc ccgataatga ggcgcagcgc
    46141 ctggcggttc taaaggaata tcacgtcctg ggcacgggcg ccgaagagcc gtacgacgac
    46201 atcacccggc tggccgccat cttgagccgg gctccgttca tcgccatcac cctgctggac
    46261 gagtcccggt attggatcaa gtcaaaggtc gggctggagc ggcaggaggg gccgcgcgat
    46321 gcggcgttgt tctgctccta cgccatcctt cagtccacgc ccctcatcgt gcgggatgcg
    46381 cgcaaagacg cgcggttcgc aaccagcgcc atggtggtgc gcccgccgca catctgtttc
    46441 tatgccgggt ttccgctgat caacccggaa gggtttgccc tgggcgccct gtgcgtgctg
    46501 gaccgcaagc cccgggtgct ttcggcccgg caaaaggagg gcctgctgat cctggcgcgg
    46561 cataccatgg cgcagttgga gctgcggcgg gtttccgatc gcatggcgaa cctgctggag
    46621 aatatcaagc tgctgcaggg gctgctgccc atttgcgcct ggtgcaagcg catacgcgat
    46681 gacgacggct actggcagca ggtggaaagt tatgttcgcc agcacctggg gaccgggttt
    46741 acgcacggca tctgccccga ctgcctggaa aagctgcgcc cgaacaagcc gaaggagaag
    46801 gcgtaagccg ccccggcccg cgtggccggg tgggcgcgtt aaccggcttt ccccgccgcc
    46861 aggctgctca ccggccggcc ctggttgggg agcacctgga tggcccggtc gtcgtaatag
    46921 cggatcaggt cgtagtcctt ctggtgggtg acctccagcc ggcccagccc gtgccgctcc
    46981 gtccaggcct gaataatcgg gatgctctcc ggctcgcaag cccgggcgga gaagattttc
    47041 accgtgaccc ccgccgccag gaggctcttg acgcgcgcga tcatttccgg aaccggctcg
    47101 cccagcgggt agggcggctg gaaatggtct gccgcatcgt cgcgcgccag ggtgccgtcc
    47161 aggtccacgc cgatccactg atgccgggcg atgaacaccg gttcggcgcc cggatccagg
    47221 gtggcccaga cgccgggctg gccgtgcgcg tcgcgcgccg tttctatttg cagcggtccc
    47281 atggggaaac actggagccc gtcggcggga agtccatccg aatctgccgt caaagcggca
    47341 cgggccggcc cggctcgtcc accgccacat agaccacttc ggcctgggtc acccgcatgg
    47401 tctcgcgggg cggctggctg cgtttggcgt ccaccgtcac ccgcaccgtc accgaggtgg
    47461 tgccaatttt tacggtctcc gtgtagaagc tcaccaagtc gccgacgaag accggcgcga
    47521 taaacttgac ctcatgcatg gccaccgtca cgagccgttt ctccgaatgc cggcgcgctt
    47581 cgacggcgcc cgccacgtca atgtggctca ggataatgcc gccgaagatg gtgcccatcg
    47641 agttggtgtc gcgaggcatc atcacgaccc gaatggcggg ttgcaagctg gccgtttggt
    47701 tcatccttcc agcatgcctc aaacgaacgc cgttggccag ttcttgcggc ggggggtggc
    47761 gttgcttccg tgtttttccg cccggccggg gcctgcacgt tccggctcgc ggggagccgg
    47821 gagcccgggg gcggatccca tttttcccgg cgagggccgc gcgcggggaa gctgcgcgtc
    47881 gccggttgaa aaagccaaac ccgccctggc tgcctggagc acaaggcggg tttgaactga
    47941 agggaactta aggttttggg attacttcac aatgcggcgg aagcagccca gtcccaccat
    48001 gaacatgccc agcagagccg ccgtggcgcc gctatcggga acccgaggat cgggattggg
    48061 atcgggcagc ggatttccat tcacctcgag cacggacttg gcgaggctga ccgcccaagt
    48121 atcaaaccta tgggtgctat caatatccat ccagacctgc aggccgctca gaatatcgtc
    48181 atagaactca ggccccagca caaacgtcgt gtaggaccac tggttgttca gtcccgacaa
    48241 cgtcccgagc agcgtcttta cgccattatt gtaagcataa atgttatcca cctgacccga
    48301 cggccaatca acgtcaaagg cgctgatgct cagggaggca gaagtaatgg tgcccacact
    48361 ggcgaatggc gcgccgctgt gcgtccatcc ccagtcctca ttccaccacc gataataggg
    48421 ggcgctcagc ttgacgctat cacttggagt gaagtagccc gtcggtgcct gcacaacgtc
    48481 aatggcgcta atactagtga tggcgtttgc cgtgcttccc agcatcagca aggcaccggc
    48541 cgtaaccgcg aggaataaca gttttttcat agctttctta gtcttctacc gtatctattt
    48601 tcgtgatctc ttgttctgcc cccatttccg gtcacgtcgg caacgggtgc cgtcccttta
    48661 acttattacc tttaggacat ctctacttta agcatatagc ataccagcag gctgttttag
    48721 gacttcgact tatggtttaa aataaatcca agtcatttaa gatcaatgct ttatagcatc
    48781 gttatatttc tatttgttgt gcagttaaca atgaatatac tacttccgac gatgtaaggg
    48841 aaaccgacgt ttttcggtgg gttgagcggg gtggatttta taagtgacgt agcatcagcg
    48901 gcttacggta aaatataaat gtgaattatt gtgtaagatt tcccagataa gttcgctgct
    48961 gtagcagggg gtaaagtcag tgttagtgga tagtggtggt aaggagtaag aaagcgtgaa
    49021 ttgacccctg tttttcggcc caggcggaca gtattagtga ggctcacggt tgaggttcga
    49081 ggcgtctggg gggcgcaaga atgcattttt accctcgacc gccggaggcg ggtttctgag
    49141 aatggctcca tgaatttgag gcctgaatcc gagaagcttt ttgccgaggc gttaaagtat
    49201 atccccgggg gggtcaactc gccggtgcgg gctttccgcg cggtgggggg ccagccgttc
    49261 tttgtcaacc gggcgcaggg ggcgcgtgtg tgggacgtgg atggcaatga atacctggat
    49321 tatgtcggca cctgggggcc ggcgattctc gggcatgcgc atccccgaat cattgaggcg
    49381 gtgcgcacgg cggcgatggc cgggacgagt tttggcatcc cgaacccgct cgaggtgcgg
    49441 ctggcgaagc tgatttgcgc ctgggtgccg agcgtgcaaa aagtgcggct gtgcaactcg
    49501 ggcaccgagg cgacgatgtc ggccattcgc ctggcgcgcg ggtttacgcg gcgggacctg
    49561 attatcaagt tcgacgggtg ctatcacggc catgcggact cgctgttggt caaggccggc
    49621 tcgggcgcgc tgacctttgg ggcgcccgac agcgccgggg tgccggcggc gttcacgcag
    49681 cacacgcggg tcctgccgtt taacgatccg gaggccgtca aggcggcttt tgcggcgcat
    49741 aaaggccagg ttgcggggat tattctggag ccggtgccgg gcaacgcggg gttgtatctg
    49801 cctcagccgg gttacctgga gtttctgcgc gaaatcacgg cggccaacgg cgcgctgttg
    49861 atcttcgatg aagtgatgac cgggttccgc ctggcgcgcg ggggcgcgca ggagcgtttt
    49921 ggcatcacgc cggatctgag ctgctttggc aaagtgattg gcggcgggct gccggtgggc
    49981 gcatttggcg ggcgcgcgga gatcatggat catctcgcgc cgctggggcc ggtgtaccag
    50041 gcgggcacgt tgagcggaaa tccgctggcg atggcggcgg ggctggcggc gctggaggaa
    50101 ctggcggcca gcgatgccta tgcgaagctg gagcagctcg gcgcggcgct cgaagccgga
    50161 atgagggcgg ccgcccgggc tgccggcgtg ccgatgcaat tccagcgttg cggctccctg
    50221 ttttgcggct acttcaccgc cgggccggtg cgcaatgtga gcgaggcgct ggcgagcgac
    50281 cgggcgcggt tcgggaaata ctttcacggc ctgctggccg agggcatcta tctggcgccg
    50341 tcgccgttcg aggcgggttt tctctcaacg gcgcacacgc cggcggacat cgagcgaacg
    50401 gtggcggcgg cgggccgggt gctggcggcg ttgtgagggg cggcggcccg ggtggttttt
    50461 gtgcgtggcc cgggcgccag tttgcggcaa actggcggca tgcgcattct tgcgatagac
    50521 cacggcagcc ggcgtatcgg cattgccgtc agcgatgagc tgaagctgat cgcccagccg
    50581 ctggagttta ttccggcgga acccttcacg gattttctgg cgcggctcca ggagctgctg
    50641 cgggaaaaag aggtggagtt gatcctcgtc ggcctgccgc ggaacatgga cggcagccac
    50701 ggtccggcgg cgcagaaggt gcaggaattc gtcgccgcgc tgcgggggca tgttggcgtg
    50761 ccgattcgaa cctgggatga gcggctgact tcagcccagg ccaaccgcct cctgatccag
    50821 ggcaacgtcc ggcgggaaaa acgcagggag aaggtggacc agatggccgc cgccattctt
    50881 ttgcagagct acctggatgg cctggctggc tgaatccggt tgcgctgcga tttgattgcg
    50941 gctaagggaa agccatggtt atcacgcgcc ttggttgcgg cttgcgccgg atgcccggcc
    51001 ggactggtcc ccgggggttc cgtccggcgg agcgccgggg ggcggggact gcggccatct
    51061 ctccgagccg cgggaactgg cggcgcgagt tgcttcgcgg gggagaaagt gaattatgag
    51121 aaagcgcgga tcggcgcggc gtcaaaccgg gaaagtcgag gggatgcgcc ggccccggcg
    51181 cccggaaacc gctgtccgcc ccgggaacgc cccggtttgc cccggcgctt cgccggcccg
    51241 gatgaacccg ttgaggctca agctcaccat cgcctacgac ggcaccgcct acgaaggctg
    51301 gcaggcgcag aagatcggca ccggcgtgca ggagaaggtc gaggcggcgc tggcgaagct
    51361 gtttcccagc cagccccgcg tgcatggctc cagccgcacg gatacggggg tgcatgcgct
    51421 gggcatggtg gcgcatttgg aaatcccgcg cgccgaatgc cggatgtccc cgcgcaagct
    51481 cgtgctggcg atcaacgcct ggctgcccga agatgtgcgc gtgctggccg ccgcccgcgt
    51541 cccgcggagc tttcacgcgc ggtttgacgc ggcgggcaag cagtatcgct actttgtctg
    51601 gaatcacccg gcgcagaatc ccctggcccg gcaggtggcg tggcatgtgc cgcgcccgct
    51661 ggatttgcgc gccatgcggg ccgccgtgcc ggggctggtg gggaaacacg atttccaatc
    51721 ctttgccgcc aacccgggct acgccaggga atccaccgtc cgcacgctga cgcgctgcgc
    51781 cctccggcgg caagggccgt ggctgacctt tatcctcgag ggagacggct ttctttaccg
    51841 gatgtgccgg gggattgtgg ggacgctggt gcaggtgggc ctgggcaagt tttcggcgga
    51901 ggacgtgcgg cggatgctcg cccggcgcga ccggcgcgtg gcgggcatga ccgcgccggc
    51961 gcacgggctg gtcttgtgga aagtgcgcta tccgcggcag cggagggaag cgaagggcgg
    52021 cccgggccgg tcccgctccg ggaggcgtag ccgtggcccg gcggcgggct aacccatgca
    52081 cgtagtcctg ctcgaaccgg aaattccgcc caacacgggc aacgtggcgc ggctatgcgc
    52141 cgccaccggg agcacgctgc acctggtcga gccgcttggc ttcgagctga acgacgcgca
    52201 gctccggcgc gcgggcatgg attattggca gcatgtccaa tggcgccgct ggccggactg
    52261 gccggcgttc cggcaggcat tgccccggga tgcgcgtctc tggctggtgg aatcgaacgg
    52321 tccccggctt tacacggaag cccgctttgc gccgcaggat tacctggtct ttggccggga
    52381 gacggcgggt ttgccgcgga aactcctgcg cgaaaatccc gatcactggc tgcgcatccc
    52441 gatgtttcag ccccgcgccc ggtcgttgaa cctgtcgaat tgcgtggcgc tggtgctgtt
    52501 cgaggcgctc cgccagcagg gtttcccggg cgaggtatga gccggggcgg aaacggtggc
    52561 ggcggaggcc gtgcgccccg cccggcactg cccgcctggc tcaacgctcc gtctcgctct
    52621 cccggatgcg cgacaggacc tcctccggtg tggcggcggt cagcagggct tgccgtagtt
    52681 gcgggtcgcg cagcaggcga ttgaagcggg ccaggattgc caggtgctgg gtcacggtgg
    52741 gcgcgaccag cagaaagaaa agccgcgcgg gctggtggtc aagggtgtcg tatgcaatcc
    52801 cctttgggtg ccggccaaag acgataatgg gctggtccac gaggcccacg atcgcgttgc
    52861 gcgcatgggg cagggcaacg ccgtcgccaa tgctggtgct atgcagttgc tcgcgctcat
    52921 agagcgcgtt gagcagggtc tggcgggctt ccggattttg ggcgaactgc gggatctggt
    52981 tgaccagttc ggcgagcacc gcattccggt ccgtgctttg caaatccagg ttgatggtcg
    53041 ccggcgacaa cagttcgctc aggaccaccg catgcgagtt cttcataacg ccgctgacgg
    53101 taaagggcgg cgttcgccgg tgaaagtaaa tgtttttccg cgcccggaag ccgggccggc
    53161 agcgaagcgg gccgccggag ggaagaactg aaaaatgagc ggcccggccg ggtcgccttt
    53221 cctgactcaa gtcggctcca caccttcgga aaccccgccg cggccttccg gcttgctccg
    53281 ccgcatcctc ttggtctcgc ggcatttgcg catcttttcg gcctccatcc cgccgcattg
    53341 cggcgcaccc gctttatggg cgtccgggtc ccgaaacggg ccgcgcaaaa acagggccag
    53401 atcccgcggc aaaaaagctg ccatttgcga cttgtcaagc cgcgagccgg tggtatgcta
    53461 gacggccttt tatgaaagag aaaatacatc cgaaatatga ggatgcggaa attcgctgcg
    53521 cgtgcggcaa tgtgatcaag acccggtcta ccaagccgag gatcatcgtg ggcatttgca
    53581 acgtctgcca cccgttttac acggggcagc agaaatttgt ggataccgcc gggcgcgtgg
    53641 ataagttcca gcagcgcatg gtcaagaccc aggcggcgca ggccctcgcc gcgacgcgca
    53701 agaaaaagaa gcactaacag ccttccgttt tttccgcccg caggatgaag cgtctttgcg
    53761 ggcggctttg ttttgcgaca tggacttgcg tcctcagata gagaagttcg cccagcggtt
    53821 tgccgaggtg gagacggcgt tgagcgaccc gaaggcgttc aacaacccac aacgcgcgca
    53881 ggagttgggg aaggagtacg cccggctccg ggaactcatg ggacaggggc aggcctatgc
    53941 gaaagtcgtg gcggacctgg cggaaaaccg cgccctgctg gaggcggaac cggcggaatc
    54001 ggaactggcc ctgctggtcc gggaggaaat cacgcggctg gaggccgagg aaaagcgcct
    54061 gggccaggaa atccaacggg gcattttgcc gcccgatccg gctgactcgc gcaacaccat
    54121 tattgaaatc cgcgccggag ccggggggca ggagtcggcc ttgttcgccg ctgacctcta
    54181 ccgcatgtac acgcgatacg cggaacagcg ccattggaag gtggaagacc tggattccag
    54241 cgcctcggac ctgggcggct acaaggaaat catcttccaa ctcaacggtt ccgatgttta
    54301 ccggcgcctg aagtatgaga gcggcgttca ccgggtccag cgcgtgccgg ccaccgaggc
    54361 gcaggggcgc gtccatacga gcacctgcac cgtggcggtg ctccccgaag cgcaggaggt
    54421 ggagatcgaa ctcaagccgg aggacctgga aatcaccgtc tgtcgcgcgt ccggcccggg
    54481 cgggcaaggg gtcaacacca cggactccgc cgtgcaggtg gtgcataagc cctccggcac
    54541 cattgtgcgc tgcgcggacc agcggtcgca gcagaaaaac cgggcgcggg cgctcatggt
    54601 actgcgcgcg cgactgctgg agcgcaaggt ggcggaggag aaccggaaat acgccgacca
    54661 acgaaaagag caggtgggta ccggcgagcg caacgagcgc atccgcactt acaatttccc
    54721 gcaaaaccgt ctcaccgatc atcgcatcgg gctgacgctc tataacctgc cggcggtcat
    54781 ggagggggac cttgacccgg tgctggagcc gctgatggcg catgatttgg agacgaagct
    54841 ggcggcactg gcctcataag gcgcgccgcc ggcatctgaa aaccaggctc accaatgaac
    54901 gcatcccgac tctgtccgcc accccgcccg cccgggggga tcattttcga cttcgacggc
    54961 acgctggccg acacgatgcc gctgcattgg caaacgtggc ggctcattgc ggcgcgatat
    55021 gggctgtctt tttcccgcga gcggttctat tccctgggcg gggtgccggc gcgggaagtg
    55081 ctcaagatgc tgagcgcgga gcagggggtg gccctggatc cggtggccgt ggcccgcgag
    55141 aaagaggcgg cgtacctggc acttattgat cacgtggagc cgattcccca ggtcgtccgc
    55201 gtggcccggg agcactacgg acggattccc ctggcggtgg cctccggggg cgcccggccc
    55261 gtcatcgaac gcatggtgga tcacctcggc ctccggcacc tgttccgcgc gatcgtcacc
    55321 cacgaggatg tggcgcggca gaagccggcg ccggacatct tcctggaggc cgcccgccgt
    55381 atcggcgtgc cgccgcgagc ctgccgcgcc tacgaagaca cggatttggg gattcaggcc
    55441 attcgcgcgg ccggcatgga ggcggtggac gtgcggcagt tgctggcggc cgcgaaccag
    55501 ccagccctgg cctaattagc gcgcggcgcg cggtcggagg gcggcggcgt ttatccctgc
    55561 cccagcttgc gcgatttctc ggtcgccgcg cgcaccgcgc tgatgagcgt gccgcgcagc
    55621 ttgcctttct ccaattcgtg caggccctcg atggtggtgc cacccgggct ggccaccatg
    55681 tccttgagcg cgccgggatg caggccggtt tccaagacca tccgggcgct gcccagcacc
    55741 gtctgggccg ccaaccgcgt tgcgatctcg cgcggcagcc ccgcggccac gccgccatcg
    55801 ctcagccctt caatgaacag gtagccatag gccggcccgc tgccgctcag gcccgtgacg
    55861 gcatccagca gcgtttcttt gaccgggagg gccaggccga cggcggaaaa aagctgctgc
    55921 accagcgcgc catcggccgg gtgcgcggct ttgcccaatg cgaacgcggt ggccgaggcg
    55981 ccgatcaacg ccggggtgtt cggcatcacg cggataagcc gggccccggc gcccaggccg
    56041 gcttcgagcc gggcgagcgg gatgccggcg gcgatggaaa tcagcaggtg ccgggaggtg
    56101 aaccgatcgc gaatgtcatc cagcaccgcc gccacttgat cgggtttgac ggccagcacc
    56161 agcacctggg caaactccgc caccacccgg ttcgaaggag tcgtcggggc tcccacttcc
    56221 cgggtgaagg cggcgcggga agtcttgctg ggatcggcgg ccagagtctg ccgggcggaa
    56281 acaaggcccg cctgaatgaa gcccttggcc agcgccgtgg ccattttacc cgcgcccaga
    56341 aatcccaggg aaagcttttt ggacatgcca gaagggtagc agcccacccg ggccgatgaa
    56401 aaggaaaact gccgttttct gcccaaagaa tcgcatcccg aagccacggg ccgggctgag
    56461 ggccaatgcg taagcaccac attggcagca ggttatcaag gtataagcat ggcggcgccg
    56521 gcctggtttt agccgtggca ggcggcttga ttcgggaaag ctttcctggc tttgcagcct
    56581 ttaatcaggt cccgctgccg ggccgcccgc ggccgtcctc gccgaattct tcgctgggaa
    56641 aggctgttca aaaatatcgg caaataattg cagaaagaca ttgacagaat ccgggataat
    56701 gtctaaattg agagggcact agagtgcttg aggtttttta cctcggttcc tcccgaccca
    56761 gtgccgatcg tgcattgtcg gcgctgggtt attctgtaga tacccgcggc cgcaccaaca
    56821 agttcaacaa cgccggcagg aaggtcaagc cggcaatcat gcaggttgcc agcccggtgg
    56881 tcatgacgca ccccaggctg tagatgccct gatgccgggc cagcatcagg ctcccgaagc
    56941 ccgccatgga agtcaggcag gataccagca ccgccttgcc ggtgctgcgc gccaggatgc
    57001 tggggctttg ctcttccgca aaccggttga ggacatggat gccattggtc acgccgatgc
    57061 cgatgaccag cgggagggtc atgatgttgg ccgggttgaa cgggacatcc agccagccca
    57121 taagcccgac cagccagagc gagccgattg cgaccggcac cagcgccagc aggacgctgg
    57181 tgaggctgcg gaaatggaaa aaaaccaaca gcccaatcgc ccccagggaa taccaggctg
    57241 cctgttcata gctctccttc agcagggcgg tgtaatagta gagttgcacg ggggttcccg
    57301 tgacgtccgg atagattttc tccacctgcc ggatgaaggt ttcctgcacc tcgcgctgcc
    57361 agatatcgcc ctggggatag accatcagca ggtattttcc cgtgacgccg ataaagcggt
    57421 cgcgcaaggc cggcggcagg tccgccacgc tcagcggcgc gcgatcgtcc tgggtccgca
    57481 gggcctgaaa ggtatcgcga atatcgagga acaggcggcg ttgaaactcc gccagcttga
    57541 gcgcgttcgc cgccacctgg cggtcattgc cccgcagcat ttccttgcgc agcgcctgga
    57601 tggcgtcgcg cagggattcg aattggggaa ccaactcggg ttgttcggcg cgcacggctt
    57661 ccagcgccgc gcccaggtag ccgcgcaggg aataaagaac ggcgctgagt tcctggagtt
    57721 ccaccgggcg gggatccggc tcgctaaagc gcacggccgc cagctcctgc ttgatctgcc
    57781 ccaccacagc cagtttgcgg gtttgatcct cgctcagaaa gccgttaatc gagtccacgc
    57841 tggccacggc cgaaagattg gtcagttggc gttccaacgc gcccgcctgc gccaggttgg
    57901 tggccaccac ggctccaaac aaaaccgatt tgggcgtcga ggcgatcagc gccttctcat
    57961 actccaccgc cggcaggccc ttgctttgca tgttaaccag attgtaatcg aaataaacct
    58021 tgcggagctg cgtggccgcc agtccgcata gcgccagcgt gaccccggcg gtcaggacgg
    58081 ggcggcgcag ccaaaggttt tcgatccacg cccggcgttc cgcggccggg cttagctggc
    58141 ggtccaccac gttctggcgt ccgcgcaaca acagcaccgg caggagcgtc atcatcggga
    58201 ccaggcagat gaccaggccg ccgccgcaga tgatccccat ttcctgaatg cctttgaagt
    58261 tggtcaggcc catcgccaga aaccctccgg ccgtggtcag tccgccggtc agaatcccct
    58321 gcccggtgag caccatggcc ctggttaacg ccgtctccat ggcctcgccc cggcgcagct
    58381 cctcttcata ccgcgagatc aggtgcacgc cgtagtcaat ggccaggccg atcaggatcg
    58441 gcatgaaggt gatggtaagg atattcaggt ggccgatgac cagggtcgca aagccgaggg
    58501 tgtagcccag gccgataatc aggcaaaacg tcgcctttac cggccgcccc gtctcctggt
    58561 agccatagat gaaaatcagc gcgcacaacg caagcgcgac gacggacgcc agggcggtgt
    58621 ccttttggga ttgagccatt tcatccagct ccagcaccgg ctcgcccgtc agccccacgt
    58681 taatccccag cacctcgagc ttggtctcct ccaccagctc ccgcaagcgc agcaccgccg
    58741 cggtgttcaa ttcctctgtc ggcgcctggg cggtcaccag gtaaatccgt ccctgggcga
    58801 aggtgatgta catttgctgc tgggcttctt cgcccgggtc gaacagggcg gtgatgcccg
    58861 gggaaggcgg cgcgcccgag cggcgcaggc tatccgtagc ctgcgtgatg atgcgctcca
    58921 gcgccggcag cgccttgagg agcgattcgt tggcttcgga agcctcctgt tttgccgtcc
    58981 ggaactgggt gcagatcagg cgaaagagcg acacgaggtt cgtcgtgcgg gtgaactgcg
    59041 cgataaatgg ctggaaatcc ttcaggatca ggcgcaaatc ggccaggtca ttttcaggca
    59101 ccagcagcag ggccttggac ccgagcatct tgaggtctcc cttgtaaaag acatggttaa
    59161 acaggttggt ctccgcttcg agcttggcgc cgagccgatc cacaaactgg cggttcttct
    59221 ccccatcctc gctctccacc accaccacga ggtcgtcctg ggtcggaaac tccttcttca
    59281 gccgcagaaa gttctggtga tacttcttgt tcgcccccac gaggtcattg cggctcgtgt
    59341 tgaactcgat ccccggatac ttgagggtca ccacgaggca cccggcaaac agcaccaact
    59401 gcggatacag gaacagccgt ggatgccgca gcaccacccg cgccaggccg gcgaggaacc
    59461 gggccatcag gttgtccgtg ctaagcggtt taatgcgcat gggcgcgacc gggctggcca
    59521 ccggtgcagg aaaggttccc acgacgcgcg gctggcttcc cggaggccgg gcgtccggga
    59581 ggcgttatcc gccgctcccg gcggcgccct ccggcgattc ggcgttggaa taaacttcct
    59641 tcacgtcgtc gtggtcttcc agggcctcga tcagcttgtc caccgcggcc accgcctggc
    59701 tgtccggcag cgcgacggtc agcgtgggca gctcggtcac gcccgccacg tcggccttga
    59761 tgccttgggc ttcaatctgt ttatgcacgg cctcaaactg cgccggggtg gtgatgattt
    59821 cgtagccttg cggctcggcc ttgaaatcct ccgcgccggc ctccagcgcc agctccatca
    59881 actgatcctc gctggcggcg tcgcgcgcga tgatgatctg ccctttgcgg tggaacaggc
    59941 gggtgacggc gccttgcgag gcaatgctgc cgccgttctt ggtgaccagg ctgcggattt
    60001 ccgaggcggt gcgattgcgg ctgtcggtgc tgatctccac caggatcgcc acgccatgcg
    60061 gcccgtaaat ctcgtaggtc aggtcttcaa agttcgccgt ctccccgccg ccggttcctt
    60121 tcttgatggc ccgctcgatg ttatcgctcg gcatgttggc gctgcgacac ttgagcaaaa
    60181 ccatccgcag gcgcggattc atgctcgggt cgcccccccc gatcttggcc gcgatgctga
    60241 tttccttgct caacttggaa aagattttgc cgcgtttggc gtccaacgca cctttgaagt
    60301 gcttgacctt tgcccatttg ctatgtccag ccatatctcg ttattggttg caagtggaat
    60361 tgaacaccac ttgccccccc ggcagaagct atttttttcg gtgccgcgcc cgccggccat
    60421 cccggcctcc tccctcacag cccgaagccc agggcgggaa aaccatcccg ctcaggcccg
    60481 aaccgggttt ggctcgatga gatggctccg ccgctggggc gggccgcggg ggcggtatcg
    60541 agcagggggc gatcaggggc tccggcccgc tcccgggggc cggaccacat tcgggtccag
    60601 gcgcgcggct ttgttgaact cggcgaccgc ttcctcccaa ttaccctgcc gggcgagcgc
    60661 ctcgctcagc agcaggtgaa ccggggcgct ctccggtttc agccgcgccc acttgcgcag
    60721 ataggccacc aactgcgcct tctcctccgc cgtctgcacc acctccgccc agtcccgggc
    60781 aaagctcaga tgcagttcca tatacagcgg gatggtttca tacgcccgcc ggtaaaccgc
    60841 gtcccgcagg gccacgtcca gggcgcggca gtaaagcggt tcggtgctct tccaaagcgg
    60901 caactggcgc gcggttgcca ccgtgctggc ggccagaagc accgccgcgc cggcgattac
    60961 gaatcctttg gcccggcgat gtttttcggc caagtccccc aacccccagg ccaggatgat
    61021 caggagcccg atggacggca ggtaactgta gcggtcggcc caggattgct gcccgacctg
    61081 caccagcccg atgaccggca cgagcatgcc caaaaaccat aacccgccca ccagccagta
    61141 gcgccgccgc cgccccagcc agaacatgcc gaggcaaacc agcagcaaaa cgcccgccgc
    61201 gccggccacc tgctccaccg gccacgcgcc gggatgcgga tacagaaagg ccaactggtc
    61261 cggccagatc attttccgga gataacggac ataggcaacc agcgcattgc ccaggcgcca
    61321 ggaaaggcgc agttgatccg ccgtcgccat cgcgccctcg gccttttgcg cccacagggt
    61381 gatcccgctt tgcaccaggg ccagggtcag gaaaggaact ttctccagtg ccagcaaaga
    61441 ccaggcactc attcccgcag tcgagccggg cgcgcgctgc ccgcggcggg gaacccgctg
    61501 cagcggccag aaatcgagca gccccaggac cagcggcacc gtcaccagca tgggcttgct
    61561 cagcagcccc agcgcgaaac aaaccagggc cagaccataa aaaagggacg caggcaacgg
    61621 cgggcggccc gcgccgcgcg ccgggacggt cgggtcccgc tcctccgggc cgggcggccg
    61681 ggcgtagcgg gcgtaggccc agagcgtaag catgaaaaag aacccgctca acacatcctt
    61741 gcgctcggaa atccaggcga ctgactcgac gcgcagcgga tgcagcgcga acaacgccgc
    61801 gaccagcgcg ctccgccagg tcgcgccggt caactggcgc aacgccaaaa acagcagcgc
    61861 cgcgttgagg gcgtgaaacg tggcgttgac gagatgaaac cagcccgcat ggacgccaaa
    61921 caactggcaa tcgagcatgt gtgagaccca ggtcagggga tgccaattcc ccgcctgcgt
    61981 cgcccgcaac gcccaggcca gccccggccc ggtcaacccg gctttcaccg ccgggttgtc
    62041 gagaatgtat tgcggatcgt cgaaccgaaa aaactcgaag cgccacaccg gcgaaaacac
    62101 cagccaggcg cccagcgcca ggagcaagct gattccccac acccgcccgc ggccgcgagc
    62161 cgccgccgcc ggcagtgccg cgcgcgtttc cggttgcggc gggccgccgc tggcgcgtcc
    62221 ttgcgccggc gcgggcgcgc cgggtttatg acgacgtcgt ttcatccgct tcgcagtcgg
    62281 cattctcatt caaatcggga atcaaggcaa tggcaacccc tcgcttcagc gccagtactg
    62341 tcagcgccgc tgccgcgcgg gccgccggca agcgcccgcc cgaaaaatcc acgccgcccc
    62401 ggcgcgaaag ccgcctcgca gacgcaaccg gcgcagcgcc atttttcggc aatgcaattt
    62461 tagggcttgc atttttcaac ggacggagcg aggttatcac caggcaagcg cgttcgggtg
    62521 cgcgaggggg tcggaataac gcgcgttctt tggaatgaaa gcgatagcag attttaccct
    62581 gcctatgcgc gtgattagaa actatgtcct tgaaccgtag taaataagcc aagggactca
    62641 aactgtggcc gtggccgaca tgcctttact ggaagtcgaa agcagcccgg tcagtcccac
    62701 ctgtttctaa taagttcaaa ggaactgtta cacacggcac tggcggggtt ctttttttcg
    62761 ctccctcaac ccggaaatac cgactcattc ccccagcgat tgccccgatg aacggcgaga
    62821 acggcccgat ccccgccgcc gttgcactca acgttttccc gacgccctcc aatcctcgtt
    62881 gccagctcaa ttccactccc acaagcccga ccaggctatt tcccaacacc aaaggccatc
    62941 cagcctccct gctcgcagca gcaagtctat ttaatgcgtg ataaatagct gaaggccccc
    63001 gatctggagg tggaggactt acattccagc ttccttccgc caaggccaaa acgccatcca
    63061 aggttcgccc aagtcgcgtc cctgataggc gaaggattgg gctctgaaaa cagggaaaca
    63121 ggcaggaaag tgataaaaga agaatgacaa agggggtttt gagggaaaat caggcaaaaa
    63181 aggccgaatt agccctaatt taatggttta acccagtaac gttaacgata caggaacaag
    63241 gcggaactga actttaaggg tttggccgct gttttggcca ggttttatgc tgaaatcgtt
    63301 gccggatcgc ggcgattggt ttcctgtacg aaagattatg gggatttggc tggcaggagg
    63361 ctcggatgga tgacgcgccc ggcggtgtag atgacctgcc cgaaaggcta aagggcgttc
    63421 cccctcacgc cgacggcatg aagctctttc ctcgtttcga ccttttcagg catccatgcc
    63481 catgacccag atgccgtgaa tttaccctgc cactgagccg tgtacaaggg gtgtacaa
//
LOCUS       DAOPQG010000004        58834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_683_length_58834_cov_14.582878, whole genome shotgun sequence.
ACCESSION   DAOPQG010000004 DAOPQG010000000
VERSION     DAOPQG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 58834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58834
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_683_length_58834_cov_14.582878"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(508..1530)
                     /locus_tag="PKI07_00775"
     CDS             complement(508..1530)
                     /locus_tag="PKI07_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC49613.1"
                     /translation="MRTTFGQPGPEDSGGTALRAPGQTRPQAPRKTPARESPALARGS
                     SEMNDDRSLVEALNNSVVFQDYARAFTEATGLPVALRAVESWQLPHHGQRNESPFCAV
                     VLETSRACASCLSVQEQLSAAAAQQAHTLTCPAGLSETAVPVRLGDRLIGFLQTGQVF
                     RKPPGRAQFQRIQRLLAAWGVRASPRKIEQAYFSTRVVPGKQHDAVVKLLTIFAQHLS
                     LLSNQVVLQQSNAEPLVISRAKKYIQEHQTENLRLGHVAKAVNTSTFYFCKIFKKATG
                     LNFTDYLSRVRIEKSKNLLLNPNLRVSEIAFEVGFQSLTHFNRVFKKILGQSPTEYRA
                     QLLGKS"
     gene            1636..2508
                     /locus_tag="PKI07_00780"
     CDS             1636..2508
                     /locus_tag="PKI07_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOC49614.1"
                     /translation="MKARHLVFLVLMNCCWAGVYSAYKVIGQSLPGGSIITLRFALAA
                     GCMLLIWPWLQGPAPRGRDLFYASLMGVLDFVIAQRLQIYANAIGSAGNSCVLMAAQP
                     LFTSVAAALFLREHIGPRRLGGFGLSILGVIILNRVWRSDFQWAGLLPSLIFISTFFC
                     ETAYSILGKPIVTRASGAKTVAVSLLAATATNLLIDGPRTLSAAKSLPPHVWWLLLGL
                     AVICTVVGYTLWLVVIRQCPVNVAVLTVFVQPICGVALAAWWLGEPAHWGHILGSLTI
                     LAGLILGLSRQIHR"
     gene            2605..3768
                     /locus_tag="PKI07_00785"
     CDS             2605..3768
                     /locus_tag="PKI07_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOC49615.1"
                     /translation="MTTLESTARRNEPPRVEGLGAEQRPAKGASLRPIGMVRGAVCFL
                     MIFGAGCFGPPALSHGATGDSGAYTNPVYAASMPDPSVIRHEGIYYAFGTTGNSRTPD
                     GRIFTVLRSRNLVEWEALGGALKPPSPNRQVQYWAPEVVFHRDTFYLYYSMGGLEPEK
                     FELRVATSSRPQGPYEDAGRRLLDCETNRFTIDAFPFRDDDGQWYLFYARNFTNSAPG
                     QHPGTALVVDRLLDMTRLAGDCRVVVRARYDWTLFEAHRRMDVYGATFDWHTIEGPCV
                     VKHAGRYYCFYSGANYQTDRYGVDYVVADHPLGPYTGQGDRARVLRSVPGHVRGPGHH
                     SIVPGPHGQSQYLVYHAWDPAMRVRWMCLDQLLWTPEGPRGAGPSWTPQPAPR"
     gene            3777..6806
                     /locus_tag="PKI07_00790"
     CDS             3777..6806
                     /locus_tag="PKI07_00790"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOC49616.1"
                     /translation="MQRKRMRYLWVAGLLLGLGTHAPAEAPGDALARGFADPPISARL
                     RAYWWWLNGNVTKAAITRDLEEMKAQGFGGALICDAGGAQQDGNDPVPHGPTFFTPAW
                     RELYKHTLREADRLGLEMSLNIQSGWNLGGPMVRAEDAAKKLTWSEARLTGPAQYAQA
                     LPAPKARDHFYRDIAVVAYRLPPQDGPASEGQGTARPPARRPLQNWEQKALHRPLHFS
                     APDTAPLFAEEKDVPGEEDAQARQVLDLTRNLSPEGVLRWEVPAGHWQVLRLGCTIGD
                     HSRVSTCSDGWEGYAVDVLDAGAFNRYWDAVVEPLIADAGPLAGRVLKYLHTDSWEVE
                     PLNWGPTLLAEFRKRRGYDPLPYLPVLMGRIIDSRAASNRFLYDFRKTVGELAIDNHY
                     RIFRARAHRHGLLIHPESGGPHAVPIDAQRCLGMNDAPMSEFWAWSWRHRVGDHNRFF
                     VKQPASAAHTHGHRLVLAEGFTTIGPHWQETLWDNLKPSFDRALCEGLNLLVWHAFVC
                     SPAEMGLPGQQYFAGAHFNPNTTWWTKSGPFLAYLNRCQFLLQQGRFVADACYYYGDH
                     VPNFTQLKRTDPARVLPGYDYDVVTEEVILRRMTVREGRLVLPDGMSYRVLVLPERPG
                     LSLAVLRKLRELVQAGATVIGPKPSQTYSLSGFPKSDAAVRKIADELWADEAQGRRGQ
                     RLRGRVISGQTARAVLAADGIPPDFECRLQAPPPPAAIPNANLGAAGLADAWGDPRTA
                     AAGSGSGSLPPLDYIHRSTGEAEIYFVASRSNAWLRADCSFRVVGKAPEIWDPIHGTT
                     RPAAAWSSQGSQTTLPLEFAPYGSMFIVFRQPQGGGNLPAPRRNFPACGVAHELGGPW
                     TVKFDPQWGGPSSVEFERLVSWPQRPEEGIKYYSGTATYFKTFDLPESLRRPGLRLAL
                     DLGQVANLAEVRLNGKNLGIVWAMPFRLEVTDALKPEGNQLEVEVVNFWPNRIIGDQF
                     LAPEKRFTRTNIRKLTKDTPLLESGLLGPVRILVQEH"
     gene            complement(6895..7932)
                     /locus_tag="PKI07_00795"
     CDS             complement(6895..7932)
                     /locus_tag="PKI07_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOC49617.1"
                     /translation="METILLLINTEADGSLAKPAFEALGAAKALRAGLPEAKLAVGLV
                     GQTVQSGADQIGACGADAFLGVAGADFASPRYASDAAAAEALVKAAQATLIIAPATAR
                     WNRVLPGVAQRLGGRADTHVTGLSAASGAITINRWYYRQRMEASLQRTHRPWFILLDP
                     GSQSAWQGAAATASVRILPVTLSEACQRTTVVGVRELAADAQTIRPDADLLLVAGAGW
                     TKKQADGQTHVKEAEELILGCLKLARASLGSSKSLVDLGGEGQAVLSFLTHLNQIGQT
                     GSTPRHPKGLATCCHGEEPHTVGWRFINERRAISLDPNCGWARGKADVLYVADAFAVL
                     ARLNQLLAAAS"
     gene            complement(7948..8757)
                     /locus_tag="PKI07_00800"
     CDS             complement(7948..8757)
                     /locus_tag="PKI07_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOC49618.1"
                     /translation="MSNEYQIVVCGSIVPDPLQTLEPVSGPDGPSLRNEMMLPAVLDP
                     WASHALYEAAHLAKNTPGSKVWLVSLGPKAKLQQVMMTVAQKVPFELIALDGPASGFA
                     EAAEVATVLAEAIAAIPGLDRSKLLLFGGWESASRGAGVTMQMVAERLGILDQFQGVD
                     ELKVLPGGALEILERVEGGKQQVSRCAGPPALLGWATGNLPEPRNNPQVGMANMRTVM
                     PALQKAKPVKLAAAGVSFASVALPKQLRETRIVKDKSPDEIAREIFEWIKR"
     gene            complement(8747..9436)
                     /locus_tag="PKI07_00805"
     CDS             complement(8747..9436)
                     /locus_tag="PKI07_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49619.1"
                     /translation="MGTVSCGTSFPGTLPQSPRKSIPFRRPAAVIPDAGPGRVAKIFG
                     KGLNKDHNISVSSIQFNRNCSGSGIWVDARPVFHSGWLKQGRLVQNNEPPLVVSQPAF
                     SLSLARELPLLETKDSRLTLCRSYVTVLVEERFSSSFSLNLSFLSAVPLHPPCCCQTY
                     DCVNDIGPGNPAHRGGTVTGWAMSALDRRSKTTRNAGHERSRLLTRTLASLFLAFQTA
                     REIGLSASHEQ"
     gene            9421..12159
                     /locus_tag="PKI07_00810"
     CDS             9421..12159
                     /locus_tag="PKI07_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC49620.1"
                     /translation="MKQFPYLRERGKKVVGFGLLLALGCLPWTGALAGPPTVQELVDQ
                     FSMANYCNVVSNKLYTRKGMNRAPVQVGGAQHDLCRDAIYDEFQQVGLNPYKDPFSYV
                     DTMGPTTVHVCNIIAVKEGVQNPNNEVYVLGAHYDSRENPGADDNATGVGCALEIARI
                     FAQHHFAKTIVFAIFDSEERTEAVTGRHRLGSLRYVGQHLNDNIKGMISVDMIGWQAP
                     SPNRNKAYICGRPAFNTIRYELQAALTNYGGLNGVISTSDNMSDHYSFEQAGFSACCL
                     IEYNWSGNSRYHTANDYVEAPGYLDWDYLEKMCKGTIGYFAAQLQPVDVTPQVLSVQF
                     DAGGCIIHGQGLALCQYAIDRCTDLITPDWVAIGTNTAAAVDGTFAVLDPGANPNSQP
                     AGFYRARFVSGYTGGPGNLPQITVQPLHRVVDTGGATSFSVSAIGDPPLNYQWRLNGN
                     AIAGATNNTCVILDAQAAAAGDYSVVVANYSGSVTSKVVSLTVYPPQTQVFADDFDSD
                     TSANWTLSQSSTDTRCTFNYDYSADGIPSAPNSVGGTTRGVKFEANMTKGITAALSMS
                     PVGLSVGGDYRLCFDMWINANGPFPLGGNGSSEYITGGLGTTGGHVQWDRAGSATDGY
                     WFAVNGEGQAVDTSVISDYGAYARTNVQTAASGVYAAGTNNNSRGNLNGYYVTAFPGQ
                     APPLLQQATYPQQTGVLEDGAVGFAWREVIIAKRGNIVEWSIDGVKLAAFTSASVVAS
                     NIFVGYWDPFVSISDNAALSFGLVDNVRVEWATNSTPPTDVIIIDNPAATVAGQWTNG
                     VMSTDKYGDDYFYAFAGTGTQYVEYRPNLPASGNYAVYEWHPQGANRTTDAPIAIKYH
                     GGSQTVRINQQINGGQWNLLGTFNFAAGTAGYVRIQDNFTKGTVVMADAIKFVYAP"
     gene            complement(12195..12932)
                     /locus_tag="PKI07_00815"
     CDS             complement(12195..12932)
                     /locus_tag="PKI07_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOC49621.1"
                     /translation="MSTPAAHLSPAARAALPRHVAVIMDGNGRWARQRRLPRIEGHRA
                     GAESARVIIRTAGELGIKYLTLYAFSAENWNRPKDEVDALMKYLVHYLKTETPELDQN
                     NVQLQIIGQVDRLPARVQSHLQKSIATLAKNNGLTLIMALSYGGRTEIVDAARSIAQK
                     VKAGELDPAGITEQLFARHLYTRNIPDPDLLIRTSGEMRVSNFLLWQISYTELVVTPT
                     LWPDFRRPQFYAALEEYHRRHRRFGGL"
     gene            complement(12949..14217)
                     /locus_tag="PKI07_00820"
     CDS             complement(12949..14217)
                     /locus_tag="PKI07_00820"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOC49622.1"
                     /translation="MANTILVGAQWGDEGKGKIIDVLTDQADVVVRTQGGNNAGHTVH
                     IKGQKYILHLVPSGILHRKKVCMIGNGVVVDPVSLVEEIEGLLQLGIRPHGNLYLSET
                     AHLVLPYHRELDAQRERLKGKNRIGTTKRGIGPAYGDKAARTGLRVIDLVNPERFRQQ
                     LRLRIKENNDVLRAYGARPLSFERVHRQYRAAGDYLKPFVANTVILLQEALRRDADIL
                     FEGAQGTFLDLDHGTYPFVTSSNTTAGGACTGSGVPPHRMDRVVGVMKAYTTRVGEGP
                     LPTENAEIADLLHALGREFGATTGRPRRCGWFDAVVTRHACLVNGIDHLAVTNIDGLD
                     SLETIKVCIGYRDGDRRYDYVPNDIEALARCTPIYAEFPGWRTPTHRARRWKDLPAKA
                     RSYLKALAELSGAQLIIASVGPGREQTIML"
     gene            14433..15038
                     /gene="thrH"
                     /locus_tag="PKI07_00825"
     CDS             14433..15038
                     /gene="thrH"
                     /locus_tag="PKI07_00825"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOC49623.1"
                     /translation="MKQCIVTLDMEGVLTPEIWIAVAEKTGIKELRRTTRDEPDYDKL
                     MRGRLNILDQHGLKLSDIQRVIGTLSPLPGGREFLDELRSLVQVIILSDTFEQFAAPL
                     LRQLGWPTLLCHRLVVEQDRIVDYKLRVPEQKQKAVAAFKLLNYYVIAGGDSFNDTAM
                     LCEANAGFLFRAPDNVKQQFPQFPAVETYADFLGLIQRAMN"
     gene            complement(15065..15706)
                     /gene="plsY"
                     /locus_tag="PKI07_00830"
     CDS             complement(15065..15706)
                     /gene="plsY"
                     /locus_tag="PKI07_00830"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOC49624.1"
                     /translation="MLTLLIAYLLGSIPTGYLVAKARGVDIRTVGSGNIGATNVFRIL
                     GTPAGVFVLLADAAKGWAAVFFVAQLLWGACGAAAGGQTLEWFRLGAGVAAILGHNYT
                     CWLRFKGGKGIATSAGVLLALVPMALLIALGVWIVVFALSRYVSLASICAAIALPFAA
                     GWRGESKTLTVVISALAVLAIYKHKGNIQRLLNGTENRIGRRAPPPPDAPPKV"
     gene            15959..17017
                     /locus_tag="PKI07_00835"
     CDS             15959..17017
                     /locus_tag="PKI07_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC49625.1"
                     /translation="MNAEHEPPETGPVRVAVLGVGSLGKEHARIYAELAAAGQIEFAG
                     VYDIADETAQRIARKHAVRTFGSVAEAAAASDALSVVTPTTTHFALAQELLRQGRHVL
                     LEKPMTDNAAQAAELVQLAQQKGCVLQVGHVERFNPVFQYLETVATDPRFIEAHRLSP
                     YPARSTDVGVVLDLMIHDLDVVLAFVKSPVQSVDAVGIPVLSPSEDIANARLRFANGC
                     VANLTASRVSPERMRKIRVFSGGAPASYISLDYRAQEGYIYRLARNDEEESSLLKKLL
                     HARDAAIVSEFAGQRIVREPVPLAREEPLRLELQHFIQCVRAHRTPRVSGESAKRALD
                     LALEITRQVQTAGLGQNR"
     gene            17120..18340
                     /gene="lpxB"
                     /locus_tag="PKI07_00840"
     CDS             17120..18340
                     /gene="lpxB"
                     /locus_tag="PKI07_00840"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOC49626.1"
                     /translation="MRPGSFMLIAGEASGDALAAELVHALRQEYAEAPAVPTTDYQPR
                     LTTLAPRFFGAGGPQMAAAGVELDFDMTAHSVIGLSDALRHYGKFRRLFRRLLQLARA
                     RQPEVIVCVDFSGFNRRFARAIRQGTRPRADGFHNWKPRIIQYVSPQVWASRAGRARQ
                     MARDYNLVLSIYPFEKDWYAQRVPQLPVEFVGHPLVDRYGPDPAAQDESRAREGGPLL
                     LMLPGSRAAELRRHLPVMLSALDLIRAKVVGLRARMVLPHDALAQQARALGLPRNLEL
                     RIGGLSRALAEADVALASTGTVTTECAYFGVPTVALYKTSWSTWQIGRRIVTVKYLAM
                     PNLLAGEEVFPEFIQSAANAGNLAGAVVALLRDEARRARIKARLREVAATLGPPGAAR
                     RAAQAILATFQGRP"
     gene            18919..19542
                     /locus_tag="PKI07_00845"
     CDS             18919..19542
                     /locus_tag="PKI07_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49627.1"
                     /translation="MAVALAFFARPGAAHTLPISYLRLVPEADYLHLELIFNPFELAL
                     MAEVDDNRDAELDLGELARHGQVVADAVVGALKLRAGGAVLRPETAGMDPDLSGHHVR
                     LRAHYKVDARGLPLTVESDLPALTSPSHLTQVTYDNGTNRQLAQFDSHSRRVTFQPPA
                     PLTSAVAPAVSRGRAAASVALLALVAFLLVIAGAALGWCLRRRRARR"
     gene            19588..23055
                     /locus_tag="PKI07_00850"
     CDS             19588..23055
                     /locus_tag="PKI07_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HOC49628.1"
                     /translation="MTTKTSCSAGWFLAFTLTFGAATAPAAAPPPALWTLGTPDGAAM
                     EFAPGGREELTFTVGQSVVSKDFAGSQAGAEDWDGKTKEKAYRIVFDLAEAPQGRYEL
                     ALDLIYHSGAPTQFKVAVNDKTGIFPIQPVGKKSAWGEEGNEMLLARQHLVVPIDGAW
                     LKAQGNQVVLTPLGLGSLSYDAIAFRRAAAAPDPAPRLEPTIFFHQRAGQLKEVCNLV
                     VPFEQRFDRAAAVVRLGPQSFSATLTNAAYDFGWWVEPIEIPAAAVAGRADLEVALAG
                     QTRRNAHDIRAAKQWKVFVCPKVHNDVGYTDLQPHVNELDNRNTDRVLDLLGEYPFYK
                     FNFETSWLVDNYMDCRTAPFQKKFFDYARADRIGINVLYLNLMTGLCTGEELYRAMYF
                     THRLHRAEGSDFAFACLTDAPSHSWFLPTLLRDVGVKAFSNGANQTRAPILHHSDLNE
                     DSPYYWEGLNGERIFMWYARSYVQLKRLTGPGWGGGISSVAYLKRSVPQFLARYARDN
                     YAPDAVMIYGAYVDNAAIPARGDAPAIEQWNREFEFPKLIVATDSDYFNYVEAHFADR
                     LPVYRGDCGAYWEDGVGSTMHATIKNRETQRVLPAAETAAALATLFDSRDRYPARDFH
                     RAWNNVMFYNEHTWGAHNSISQPGRQFVERQWEIKESYATRANLDARTLLARGCNRLA
                     QQIAVDGSSILAFNWQNRPRTAPLEIELGTGDQLVDLADNQVVPLDIYAERDGWRKVR
                     FVARDVPAMGYKAYGIRGWNPSAHKENEKLAGDTIENEFYRLTVDTRTGGLKSLYDKS
                     AQRELLDAAAPYQLNEYLYVSGGEGSLILNFTFGSPPANLMVHRPEPATITKVRKTPL
                     GQRLIVETKSQNTPKVVSEYLLYDAVKRVEIVNTVEKHEVRAKEAVYFAFPFAARQPA
                     LEYQIQNGWCRPNEDQMPGACREWFTPQNLVHVRDGDFSIAWSTLDAPLATFTDINRG
                     KWLAHLPIRNGHVYSYVMNNYWFTNYRAQQGGRFVFRYAITSGRGLGREELARFDQDT
                     RTPVLAYPFLSSFSAAISQTDRPLPARGGSFLTCDAANLEMVTLKEAEDGEGFILRFR
                     EIAGRRGEAQFKLPTFHVREAHLCNGVEVNQQKLAVAGDALSVPYKPYAFTTVRLKPA
                     KTAAKDAGR"
     gene            complement(23124..24116)
                     /locus_tag="PKI07_00855"
     CDS             complement(23124..24116)
                     /locus_tag="PKI07_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC49629.1"
                     /translation="MRPQTPTLRTTMPAAAQLLRLPNTPHRRFPGLLLILLALAGTAL
                     FGCAQSSPAPPTGARSALPGVQVLRDLEYVKGGHARNRLDLYLPEKASRPLPVILWVH
                     GGGWTRGDKTNGPAFQFATRGYAVASMNYRFSQDAVFPAQIYDCKAAVRWLRAHARQY
                     GLDARHIGAWGGSAGGHLVALLGTTAGIRELEGPGGNADQSSRVQAVVDWYGPTVFLT
                     ARARNTRHQLFGGDPRQHPEKARQASPLTYVSKDAAPFLIMHGDQDQTVKLAHSEILA
                     RALKRAGAEVTLEVIKGGRHGGFLFTNAQNLKRVEAFFDRHLKGDGATPPPRAP"
     gene            complement(24119..25459)
                     /locus_tag="PKI07_00860"
     CDS             complement(24119..25459)
                     /locus_tag="PKI07_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC49630.1"
                     /translation="MPATIEPGKSESASAVPLPSPIGRYRWVVCALLFFATTINYIDR
                     QILSLLKEILDEQIGWTNEQFGWVNSAFQFAYGIGLLGFGWFVDRFGTKIGYSVSIIA
                     WSVAAAAHALVGSFVGFIGARTALGLGEGGNFPSAIKAVALWFPKRERAFATSIFNAG
                     TNAGALVAPAVIPWMAYQFGWQSCFITAGLVGLLWLFLWIPFYDVPDKSRRVGAAELE
                     LIRSDQEPERPQEKVSWLSLLGYRQTWSFFIAKFLTDPVWWFFLIWLPDFFKKTRGLE
                     IKKSWIHIVTIYGIVTVLSIFGGWITGYLTKQGWSVTRARKTGMFIFALCVLPILLVT
                     RVGDWTAVLLIGLAGAAHQAWSANLFTTVSDMFPKKAVASVIGIGGMAGSAGGIMFPA
                     LTGRLLDHFQHQGNITAGYAILFGICGCIYLVAFALHHLLAPRFELFHLKESAS"
     gene            25594..26007
                     /locus_tag="PKI07_00865"
     CDS             25594..26007
                     /locus_tag="PKI07_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOC49631.1"
                     /translation="MKTFPVGSDKEALLSQRMAQLGVREADLAETFVRSSGRGGQKVN
                     KTATCVVLLHRPTGLQVKCQATRQQGLNRFLARRWLLDKIEERQTGRVAAERARREKI
                     RRQKRRRSRRAKERLLADKAHHAAKKEARRRVSVD"
     gene            26129..27661
                     /locus_tag="PKI07_00870"
     CDS             26129..27661
                     /locus_tag="PKI07_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOC49632.1"
                     /translation="MNLTGAFARSVERQPSKIALFWGESEHSYAQLWRQALVVAEQLR
                     QQYGVRPGDRVGLWLKNCPEFVIALFGILHTGAAAVPINNFFKAEEVNYILGDAGIDV
                     LITDAELAAHRPALEAARPGLKLFRVEQLDALAAGRGGQNSGSREGGSGANPSPKESF
                     PSPGESDLAVIIYTSGTTGRPKGAMLSHGNLLHNVESCRDVLATVEGDRFAVLLPLFH
                     SYMLTVGLLLPLLVGGSMVLVKSLHPVRNVLQEILQRQATVLPAIPQFYRSMVNAPIP
                     VPLPLRLCVSGAAPLPVQVLKEFEARFHIPLIEGYGLSEASPVVTKNPVDGTRKPGSI
                     GPPIPNVEVSIQDDAGRFLDAGQIGELCVRGGNVMLGYWRLPEETAKVMRNGWLLTGD
                     VGYRDEDGYYYITDRKKDMLLVNGINVYPREIEEVLYQFPGVKEAAVIGKPDSRKGEQ
                     PIAFVVPNEGEVLAEKALLQFVRRKLADYKVPRKVIFLPALPRNASGKILKTRLRELP
                     HC"
     gene            27758..28816
                     /locus_tag="PKI07_00875"
                     /pseudo
     CDS             27758..28816
                     /locus_tag="PKI07_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
     gene            29123..29824
                     /locus_tag="PKI07_00880"
     CDS             29123..29824
                     /locus_tag="PKI07_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC49633.1"
                     /translation="MKRLMLMLLVAATTMAVAAPVNQLARREKAAGWRLLFDGKTTHG
                     WRSFKKPSFPDQGWRVEDGWLHCLGKGGGDIITDAQFDDFELQWEWKLAAGANSGVKY
                     FVLESRNEVLGHEYQMIDDARPSGVDRGNRKQLTAAFYAVLEPVCPPVKPAGEINSSR
                     IRVQGNHVEHWLNGVKVLEYECGSEEVRKAVAASKFKNKAGFGDKVRGHLLLQDHNSQ
                     VWYRNLKVRDLANTR"
     gene            complement(29855..30316)
                     /locus_tag="PKI07_00885"
     CDS             complement(29855..30316)
                     /locus_tag="PKI07_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOC49634.1"
                     /translation="MPVTTLKQLLDKEKIKYVSIIHSTAYTAPEVAASAHIPGRELAK
                     TVIVYLDGEMAMAVLPANRKVVLADLREMTGADQAKLVPEEDFKKRFPDCEVGAMPPF
                     GNLYGMEVYVAASLAQNAEIAFNAGSHTEVIKLAYKDFERLVRPQVIPFTT"
     gene            complement(30578..31138)
                     /locus_tag="PKI07_00890"
     CDS             complement(30578..31138)
                     /locus_tag="PKI07_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49635.1"
                     /translation="MLTFRFMEKREWEPITGAKLAGFGFGFGLFLLLVLRSEPGFVFL
                     LDHANLLFHEAGHPLVGLFSSRLEPYGGTLGQLVFPCALAVSFWRRGHPLAVAGAAIW
                     FFENWLNIARYMADARRLELPLVGGGEHDWNTIFSRWQVLQYDTHIAAVVRLAGWIGI
                     AAVCAWVAWRAWQDRQRAAARANPAL"
     gene            complement(31314..32327)
                     /locus_tag="PKI07_00895"
     CDS             complement(31314..32327)
                     /locus_tag="PKI07_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015216191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOC49636.1"
                     /translation="MDLKTTYLGLKLRTPLVPAASPLSEEIDGIKQMEDAGASAVVLY
                     SVFEEQLRREQAELDDHLERGTESFAEALTYFPAPNEFRLGPEEYLQHIAKAKEAVRI
                     PIIASLNGSSTGGWTEYAKGMQQAGADALELNIYYIPTDMNAASCDVEQNYLDILKAV
                     KAAVSIPVAVKLSPFFTNFAYMAKRLDQAGANGLVLFNRFYQPDIDLEALEVRPNVLL
                     STPMAMRVPLRWVALLYGKLQASLAATSGIHRATDVLKMLMAGADVTMLCSALIRHGI
                     RQIGVIERDLIAWMEEHEYESVAQLKGSLSQKNCPNPSSFERAQYMRALAEYSTGDSR
                     STE"
     gene            complement(32352..35969)
                     /gene="nifJ"
                     /locus_tag="PKI07_00900"
     CDS             complement(32352..35969)
                     /gene="nifJ"
                     /locus_tag="PKI07_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOC49637.1"
                     /translation="MNKTYTTVDGNEAVAYVAYRCNEVMGIYPITPSSNMGEWCDQWA
                     SEGVKNIWGTVPSVVEMQSEGGAAGAVHGALQTGALTTTFTASQGLLLKVPNMFKIAG
                     ELTPTVFHISARTLATHALSIFGDHSDVMHARTTGFALLSSASVQEAMDFALIAQAAT
                     LESRVPFLHFFDGFRTSHEVSKIELVDEDIIRQMINEEFVIAHRQRAMNPDRPVLRGT
                     AQNPDVFFQAREACNPFYAQCADITARVMDKFAAITGRPYQPFQYVGDPNAERVVVLM
                     GSGCETAHETVEYLNENGAKVGVIKVRLYRPFCGKRFVEALPASVKAVAVLDRTKESG
                     ALGEPLYLDVIAALTEGIPAGWGRIKSMPKVVGGRYGLSSKDFTPAMVKAVFDNLQAA
                     TPRNHFTVGIEDDVAHTSLKFDPEFSTESDKVVRALFYGLGSDGTVGANKNSIKIIGE
                     NTNNYAQGYFVYDSKKAGAVTISHLRFGPKPIRSIYLVSKANFVACHQPIFLDRYDML
                     QDLVPGGTFLLNTHFRADEVWEHLPRLVQEGLIKKKAKFYVIDAISVAKSTGMGARIN
                     TIMQTCFFALSGVLPRDEAIEQIKLTIKKTYGRKGEEVVKKNIAAVDQTLANLHEVKV
                     PAQVTSTRELPPPFTDDAPAEITRTLGVIYGNRGDSLPVSAFSPDGTFPTGTSRYEKR
                     NLALEIPVWDEKTCIQCGKCVVICPHASIRMKVYDAKHLQGAPPTFKSTDARAPEWKG
                     MKWTLQVAPEDCTGCCLCVEVCPAKNKTEARLKAINMRPQPPIRFSERDNYKFFLELP
                     ELDRRKIKVTQLRQQQVMQPLFEYSGACGGCGETPYLKLLTQLFGDRIVASNATGCSS
                     IYGGNLPTTPYAKNKEGRGPTWNNSLFEDNAEFGLGFRVSIDKQREFACELLRKLAPK
                     LGDDLVTGLIEAQQTDEAGIYDQRERVAALKQKLAGDSSSDAKMLLAVADNLVRRSVW
                     IIGGDGWAYDIGYGGLDHVLASGHDVNVLVLDTEVYSNTGGQCSKATPRGAVAKFAAG
                     GKPLRKKDLGLIAMGYGSVYVASVAMGAKDEHTLRAFIEAESYPGPSLIIAYSHCIAH
                     GIDLDRGVGARQQRMAVDSGQWLLYRFDPRRFDRGESPLLFDSSPAKIKVQDYLLSEN
                     RFKMLTKSKPEDARRFFAQAQADADRRWAYYQYLAGLKPASGNGANAPASSASAQTQQ
                     P"
     gene            complement(36203..37003)
                     /locus_tag="PKI07_00905"
     CDS             complement(36203..37003)
                     /locus_tag="PKI07_00905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOC49638.1"
                     /translation="MKNTIKLSSPATQEFWEIPILYEDAQVLALDKPSGVPAVPDRAQ
                     PERPSLLRLLHDGIKRGAPWARAGDRSYLMATHRLDAETSGVMLLAKSKPVLIALLNH
                     FGASQPGRRHVALVQGTPPEARFQIDTKLAPHPARAGLMCANARRGKRSVTLFEVREK
                     FSGYTLLQCEPLTDRPHQIRAHLRVARLPVLGDPLYGGRELRLSQLKPDYRLKPNQVE
                     RPLIARAAVHCETLALPHPLTGEPLSITAPWPRQLAAAIKYLRKFAPA"
     gene            complement(37122..38813)
                     /locus_tag="PKI07_00910"
     CDS             complement(37122..38813)
                     /locus_tag="PKI07_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOC49639.1"
                     /translation="MFGTIRKHQTWLWAIIITLTIISFVIFFSPYSKLNDAPRGRINL
                     GSINGERINPEQFIQAQREVYLRHYFMMGDWPDDESRKAGGEIERETYQWLLLIQKQK
                     RLGIHISTDVVAQAARSMIGQFQRAGITSPDMFVQRVLRPRGFGLEDFERFVRHYLGV
                     QELIATAGMAGKLTTPQEARDLFKREHEELSAAAVFFSAADYQAQVTVLPEAVSQFYS
                     NRLATYRVPERVQVNYVKFDLTNYTAAASQELARMTNLDLEIDEAYRLSGTNFLREVG
                     AQSVAEAKTKLREARLKQLEYQIARSNAVYFANPLFDMEPVRAENLDIVAQAHGFPVR
                     ATIPFDRETPPPGLGVALDFVQKAFSRTPDDPLAGPILGSNAVYIISLNKKIPSEIPP
                     LEQIRVQVVEEYRHSQALLLARQAGMDFYQALTNGLAQGKTVADLCAHAQHPLVDLPP
                     FSLSTRQLPQIEERLNLNQLKQIAFSTPPGKVSNFQMTPRGGVIVYVKSKLPLDEATI
                     AENAPAFLNAVRLNRQNEAFNDWFRQEVEKGLRDTPLARPQAPPAMNPASDEKKS"
     gene            complement(39010..40425)
                     /locus_tag="PKI07_00915"
     CDS             complement(39010..40425)
                     /locus_tag="PKI07_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOC49640.1"
                     /translation="MQIDDHLTAQKLRPKVERLFALSAQKILSLERTWDPARGTPVVT
                     AGGQYTSRAWTDWTQGFQFGAALLQFDATGEKRFLELGRRQTIRQMAPHVSNLGVHDH
                     GFNNISTYGNLWRLMREGKIPGNEPERELYELALKVSGAVQAARWSRTADGAGYIYSF
                     NGPHSLFVDTVRSLRSLAVAHQLGHVLRGERDRSISLFQRLLDHAETTALYSIYYGKG
                     RDTFDVRGRTAHESIFNLNDGSYRCPNTQQGYSPFSTWTRGLAWAILGYAEQLEFLDA
                     GHGRRAGRANERPPAAADAGRDLEASRALFLEAARATADFYLAHTCADGIPMWDTGAP
                     HLHRLGDYASQPADPFNDWEPVDSSAAAIAGQGLVRLGNYLTRRGDAKAGGRYRQAGL
                     TVANTLFDEPYLSTRPRHQGLLLHSIYHRPNGWDYAPPGARTPRGESSMWGDYHAREL
                     ALLLLREARGDTYLTFFAADD"
     gene            complement(40431..41228)
                     /locus_tag="PKI07_00920"
     CDS             complement(40431..41228)
                     /locus_tag="PKI07_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOC49641.1"
                     /translation="MNAVAMITGASRGIGRGIALELAKIGYDLIVNYAGNAVAARQTA
                     ADCLAAAQAAGRNVRVEVCQADIAKDADRRKLVEFAKTTLGRLDLLVNNAGVAPEVRA
                     DILEASEESFDRLITINLKGPYFLTQLAARWMIAQGQADIPPANYRPRIVTISSVSAY
                     TASVNRGDYCLSKAGLSMLTALYAARLAEYGILVYELRPGIIGTDMTGPVKEKYDQLI
                     EAGLTPIPRWGTPEDVGRAVAAIAQDLLPFSTGEVINVDGGFHLRRL"
     gene            complement(41633..42604)
                     /locus_tag="PKI07_00925"
     CDS             complement(41633..42604)
                     /locus_tag="PKI07_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOC49642.1"
                     /translation="MAAKTPSASATPGDPGASGLWPGRLAAGGSPFDGITARSPRPDL
                     EAFLASPAVRELAQQICEIGRRMWQRAYVDGNGGNLALRVGPDLALCTPTQISKGFMR
                     PEDLCLVDLAGNQLAGARKRTSEILMHLQIMQRQPRAVASVHCHPPYSTGFAIAGVQP
                     PTCLTSEFELTISLATAPYRTPGTPELGRFVADLVDQHNTILMANHGVVAWSHRSVED
                     AYFKIETLEAYCHTVLIASQLGKPLKRITPAQLQELLQIKQRQGIPDPRYGLEQCEHC
                     LSTEWRPGETCAAAHCAARPANPPPGGEPDEALVQAIANQIKARTMI"
     gene            complement(42731..43516)
                     /locus_tag="PKI07_00930"
     CDS             complement(42731..43516)
                     /locus_tag="PKI07_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOC49643.1"
                     /translation="MTQLWHLPVLFVTALVAGFVDSIAGGGGLITLPALLSCGLSPRH
                     ALGTSKLQAAFGSAGASWYYERAGTVAFKECTRGFLFTFVGGACGTLVVRQIDPSFLR
                     RGIPILLFSVALYVLFKPNLGLGDRPARFRRERFDFFFGLLMGFYDGFFGPGTGTFWT
                     MAFMLGLGFNMRRATGHAKVMNFASNISSLLLFLPGGYVNYAAGLTMGAGQIIGARIG
                     ARMVIKRGVKIIRPIFVSVVLLLTLKLLYDAYSRPLIEELHRL"
     gene            complement(43513..45291)
                     /locus_tag="PKI07_00935"
     CDS             complement(43513..45291)
                     /locus_tag="PKI07_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOC49644.1"
                     /translation="MPHLCVLLLLLAAGLANAPAAEIFLRASQIGYRTRDTKLGVAFA
                     KAPLPAAFTVAEADTGKARFTGSARPITGVRWGQFEHHAELDFTALRTPGRYVLRVGE
                     AQSLPFTLGAAAYHELPDQLLEFMRQQRCGYNPWLDAVCHPFDGRTAYGPLTNGTYLD
                     ARGGWHDAADLLKYLLTSGNATAQMLLAYELAEDRQLFADKVNALGQPYPNGLPDVLD
                     EARWGLEWMLKLHPAPDQLYHQVADDRDHAGRRLPQNEIADYGWGKGGPRVVYFADGQ
                     PQGLRQYQSASTGVANLAGRYAAAMSLAYHIWRNDPRHRPFAERCLQAGKEVYELGRA
                     REGVQQGNSYSAPYRYEETTWADDMEWGAAELFRATREPRYLEDARRYARLAAAESWM
                     GQEQTGHYQYFPFLNAGHFRLYGLVDRPFRKMLAGYYREGIERCERMGRQNPYRIGVP
                     FIWCSANLTVALATQCLLYERMTGDQTYRRFAAQQRDWLLGRNPWGYTLFTGIGSVYP
                     KDPHLMTMQLTGRPIRGGLVDGPVYERIFRSLKGVAITEPDPLAAFQGPAVYHDDVQD
                     YSSNEPIMDGTAAAILLWALSSHSHL"
     gene            complement(45380..46714)
                     /locus_tag="PKI07_00940"
     CDS             complement(45380..46714)
                     /locus_tag="PKI07_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOC49645.1"
                     /translation="MITWEPLITVGIYSREPEARGCLNGRFLLPDKTAVSGEFSARPG
                     EGQLVLRAGDGRELARGPEIRLTPEGDATFTLRDVTIGINFHWERKEDETFEGALRLL
                     LNADGTVTAINEAGVEQYLKSVIASEMSATAPLELLRAHAITSRSWLVAMLERQKLTG
                     KTSGPARQGQETAEEIVRWYTREDHRFFDVCADDHCQRYHGLTKIISPKAIEAVRDTR
                     GVFLVHGGEICDARYAKSCGGRSELFSSAWEGIDVPYLASVSDAAVEHPPITSEAAAE
                     QWICSSPEAYCNTTDGDILRQVLPTFDQETTDFYRWTVSYTQGELSAILRKRSGLDFG
                     VIQRLEPLQRGPSGRIVRLRIVGSQRTVVVGKELEIRRWLSPSHLYSSAFVVQPEGAA
                     GAAPARFTLRGAGWGHGVGFCQIGAAVMASRGIPAEQIVKHYFRGAELQKLY"
     gene            complement(46711..47637)
                     /locus_tag="PKI07_00945"
     CDS             complement(46711..47637)
                     /locus_tag="PKI07_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC49646.1"
                     /translation="MNICNRRVFLGRTVISGVGAMLAPGSLARAAAPGRRRRTKPGTP
                     WAEKLGWRLSCQLYTFRDRSFYESLEVLAEMGLRRVEPAFFLPLSKERPDLKTSENLS
                     PAQRREMKERMRDLGFTMPTYYAPLEKDTSEYRKVYDFAKEMGVETLIAEPPAENFGA
                     LDKLCQEYKINLAVHNHPEGPNSKYWNPDAVIKACAGHSARIGACPDTGHWVRSGLDT
                     LASLRKYERRILVLHLKDAAESGRRDSRDVPLGTGQAQYAALLRQMYDWKWRGVMSIE
                     YEHLSPQLVQDVAQCVKFVEDFAAGVNRHPSL"
     gene            complement(47780..49876)
                     /locus_tag="PKI07_00950"
     CDS             complement(47780..49876)
                     /locus_tag="PKI07_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49647.1"
                     /translation="MAENNENGTCAQDGLERSLAFGLHFPAGKQGRLPAFWRRPLLFL
                     SSAWERFKLGRKIKTTKQTSLLIGAAAILLATLAVYWPAVQAGFIWDDDDILTANPLI
                     HAPDGLWHIWSGGKFYDYWPLTLTSFWLEWRLWGGQATGYHVTNILLHAGAAILLWRA
                     LRHLNIPGAWLAALLFAIHPINVQSVAWITERKNTLSLFFFGLALVAYLRSEQARRDA
                     EMRARKTRRPKGKSPPNRAAPFNAETFWYWTSFIAFVLALLSKTSVVLLPVVLLLCAW
                     WRSSADGQPLTKGDVGRTLPFFAAGLVLALVTIWFQYERSIGSDVVAAGDFRSRLVGA
                     GMAGWFYLARIVFPRNPMFVYPRWNFSPAEVVNYLPGVLLLGIWGLSWWHRRRWGRAP
                     FFALTYFLAMLFPVLGFFKIYFQKYSFVSDHWVYPAMIGMIALVTGVGAWLAQKLAAT
                     ARPARGRLAKALGVAGAASAIAVLGPLTWAQCGIYQDEETLWRATLKRNPRCWLAHHN
                     LACLILEKCRPELSANASAPNPASPGPELTDQLRTALFHEQAALAWKPDHAAAAYGAG
                     HALVLLNQPEEAIPLLRRALELQPDMVTALDGLAWLLATHENADSRDGAEAVRLARRA
                     VELTGEQDARLLETLAVASAEAGDFPAAVQAAHKALKLALASENQALAGTLAAQLRLF
                     QAGRPLRDRQPEPSRP"
     gene            50113..50748
                     /locus_tag="PKI07_00955"
     CDS             50113..50748
                     /locus_tag="PKI07_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOC49648.1"
                     /translation="MKIVITRMTASVCGVMMAVLAAGCASSGVKNPSGVPVTHMNADE
                     QGFVAGTGVESQDLVMVADKMSRSILGIPQIANAATPPSIVINPVDNQTRFPINKDIF
                     LTRIRAQLNSKAQGRVVFLARHQMEELEKERNRKREGQVTSSSDPSVQEFQGADYFLT
                     GSLQGLSTRTKAGTSDYILYNFHLIDARTSAIVWEDMAELKKQGLEDAAYR"
     gene            50770..51954
                     /locus_tag="PKI07_00960"
     CDS             50770..51954
                     /locus_tag="PKI07_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49649.1"
                     /translation="MKLLWQPVLLALAASLAGCATSRKPAVPPLTGDILVDAPRAIAS
                     GPARDRLIWQYRLAAAAMRQGKFDLAKQNLDEVLLTLGGIYGKDPDAKKARSYFRAEA
                     RKTFIGEPYERSMAYIYRGILYWMDGERDNARACFRSAEFEDSDAALQEYAGDWVLPD
                     YLDGLATAKLGGDGSDAFQRAQANAKGIKLPPARPEANVLFFVEFGPGPTKYATGQYQ
                     QELRFNTPASPVASAMLKVDARQIPIAPTDDVGFQATTRGGRVMDHILGNKAVFKSAT
                     DAAGSVAMLGGITTAVASRDRTTQQVGLGIALAGLATTLVSAATVPKADTRAWDNLPR
                     YLSFACLPLPVGQHVATLQFLDSAGQVQSNLTKTITLNIATTDRDKVVFVSDASITPQ
                     TL"
     gene            51951..52373
                     /locus_tag="PKI07_00965"
     CDS             51951..52373
                     /locus_tag="PKI07_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HOC49650.1"
                     /translation="MKRAFPLLLAAGSLVLLAAGCRTYDKGPYLPQTSKTPAYESKEP
                     VILMDPAVRRSVTCSGLQERTLPDGRLEIVAHLRNRENRRIEVQANCVFKDQNAFSTG
                     DETPFVMVILTENGTEDVKFTSMNNLAKKYTIRVRQAR"
     gene            52658..54022
                     /locus_tag="PKI07_00970"
     CDS             52658..54022
                     /locus_tag="PKI07_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC49651.1"
                     /translation="MNSTPNQTANPGESRRTFIKKAATAAAVVSTANLFKTPVYGQSQ
                     APAPGRVLGANDRINVAYIGTGKQGMMHITIEKKYAQDNNIAQVAVCDVYQRHLDMAR
                     NAIGVSESSAYKDHRKLLERKDIDAIVAAPTDPWHAQVSIDALEAGKHVFCEKPMTRY
                     LAEAFEVCDAVKKTGKTYVIGSQGCMDAKWHKAAEWVKAGKLGPLVWGQGSYCRNNKN
                     NSEWTFPIDPGMNEQNIDWERWLGKTPKIPFNPEHYFSWHKYYAYNSGIIGNLLPHRF
                     QPLMLATGDPEFPRRVAATGTRKVSTDREITDTTHLLAEFPSGLTLAVVGTTVNEQGL
                     PEVLRGRKATLHFASSQNRVELKPEAIFTDELEAEEFTDPQPTERIERLEKDFFDCIR
                     SGKQPVANVELAIRAQTVLCLAEMSERLGLTLFFDPKTRAIKTGDGRVVPPLSYDSVV
                     PPMG"
     gene            complement(54019..55404)
                     /gene="accC"
                     /locus_tag="PKI07_00975"
     CDS             complement(54019..55404)
                     /gene="accC"
                     /locus_tag="PKI07_00975"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOC49652.1"
                     /translation="MFEKILVANRGEIAVRIIRACKELNIRTVAIYSEADANSMHVQL
                     ADEAICIGKAPSHDSYLRIDRIVSAAEITDVDAIHPGYGFLAENAHFADVCESCNIRF
                     IGPNSRAMNALEDKAVSRALAKKADVPTPPGSEGVVESEQDALSVAKRIGYPVMIKAV
                     AGGGGRGMRVAHNDISLIKGYHTARGEAEKAFGNSGVYVEKYIENPHHIEFQILGDHK
                     GRIIHLGERDCSIQRRNQKVVEESPSPLLENPKFRKLRDRMGRAAIKIAEAANYTNAG
                     TVEFIVDNDGRFYFLEVNKRIQVEHPVTEEVTGIDLVRYQIMLAMGEPLRHSQSDIQL
                     KGHAIECRINAEDPFDNFRPSPGRIEMYYQPGGRGVRVDSHAYAGYTIPSAYDSMIAK
                     LVTYGRDRRETMNKMSRALAEYMITGVKTTISFQQAILQDPNFRRGAYSTSFIEQLLS
                     GARRELLEEKA"
     gene            complement(55471..55938)
                     /gene="accB"
                     /locus_tag="PKI07_00980"
     CDS             complement(55471..55938)
                     /gene="accB"
                     /locus_tag="PKI07_00980"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOC49653.1"
                     /translation="MDLKDIKSIIDLMRKNAVTEFELEKQDFKIRLKRGANGGGAAAA
                     HEDTPLVAYAPPLAVPPPAPVPAAAPPPAASNELEIKSPMIGTFYRAPSPESAPYVEV
                     GTEVGPETVVCIIEAMKVMNEIKAEAKGVITQVMVENAKPVEFGQVLFKIRPS"
     gene            complement(56112..56546)
                     /gene="aroQ"
                     /locus_tag="PKI07_00985"
     CDS             complement(56112..56546)
                     /gene="aroQ"
                     /locus_tag="PKI07_00985"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOC49654.1"
                     /translation="MKILYLNGPNLNLLGQREPQVYGRATLAEIEARVRAQAKKRKVV
                     VDFRQSNLEGQLVSWIQEAKGKWDVIVLNAAAYTHTSIALRDAIAAAGVPTIEIHLSN
                     VHAREDFRHKSLIAPVCLGQILGFGAESYLLALEAAVNVNKS"
     gene            56730..57053
                     /locus_tag="PKI07_00990"
     CDS             56730..57053
                     /locus_tag="PKI07_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49655.1"
                     /translation="MLSGQMIRGFDRRPGAALAGLLALLLLTATAAAGNHSWHHARHH
                     DGDGHSHFCLICSLAQGQITAAPLASISAIILPGCYWVVSRASVLRVAAFRYRFPHGR
                     APPCA"
     gene            57101..58681
                     /locus_tag="PKI07_00995"
     CDS             57101..58681
                     /locus_tag="PKI07_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49656.1"
                     /translation="MRRWSDGGVSLSVNMKAEYSIWFGASFKVHPRPRRQNLVRPRPR
                     AALPALAILLTLLAFGGGQPLLAQAADSVAELKQQLRQLQENFERMQREQRAQIEALT
                     RKLDHLAKQAAAAGNAQHPEHAPVAAPQESPPGVPASPEASTPPAAWSPAHPLTLART
                     DSAHLDISFDVLANFGGSTASRPADCLQLGHHDPNQRGFSLSAADIAFDGVVDPYFEG
                     FVNICLSLDGENETHVDLEEAYLQTISLPAGLQAKAGQFYAGFGRQNPQHAHEWAFVD
                     SPVILTRAFGPEGLRNVGAQVSWSAPTPFFTEAMLGVFNPQGETAFSFRNPEDPHGRA
                     SFKQNLRGPGDLLYVPRLASSFDLTEQQTLAAGLSAAFGPNATGAGLRTQIYGLDLYW
                     KWKAAGAPAGFPFVSWQTEALYQRYAAGADPAPALPAETLHDWGFYSQVLWGFKPRWV
                     AGLRGEYATGNRGAGDSQDVFRGERTRLSPGLTFYSSEFSKIRLQYNYDRGNLFGTEH
                     SVWLQVEFLLGAHGAHKF"
BASE COUNT        10644 a        18578 c        18909 g        10703 t
ORIGIN      
        1 ggagcgttag gctggctggg gcgggtgggt cgggtttggg cgcgggcctg agccggaagg
       61 gagggatgga cggcttgcgg atgatgtaaa aggacgtttc aaggcgggag ggagtggacg
      121 cgaacgttcg gcaaagccgg aacgggcgga cgggttttgt cgcgtgcctt ggtttttcgg
      181 cgggatgggg ccacggctgg cgaggtcatt tcccctcccg ggtggaggtt gccgcaaagc
      241 ggctgcttgc gctgccctca tcgcgagcgg cgtattcgcc cgattggggt gaggttggtt
      301 ggagatttat tcccaggttt tcccttgccg ttggttatcg caagcgcagg ccaggggcag
      361 cgcgggacgg agcgagccgc aagcacgtct ccgccgggtt tccggttcga cgatcaaagg
      421 atggtaaatg cgtccggacc cgctagccgc agagggacag agacggaata agcaagcggg
      481 gcgcgaacgg cgtacgcttg gatgggctca gctttttccc agcaactggg cgcggtattc
      541 cgtcggcgat tgccccagga ttttcttgaa gacgcggttg aagtgggtca gggactggaa
      601 gccgacttcg aaggcgattt cgcttacgcg caaattgggg ttgagcagca gattctttga
      661 cttctcgatg cgcacccggg acaggtagtc ggtgaagttc aggccggtgg cttttttgaa
      721 tattttgcag aagtagaaag tgctggtgtt gaccgctttg gccacatgcc cgaggcgaag
      781 gttttcggtt tgatgttcct ggatgtattt tttggcccgc gagatgacaa gcggctcggc
      841 gttgctctgc tgcagcacga cttgattgct gagcagcgaa aggtgctgcg cgaagatggt
      901 cagcagtttt accaccgcgt cgtgttgttt gccgggaacc acgcgggttg aaaaatatgc
      961 ctgctcgatt ttccggggcg aggcccgcac cccccaggcc gccagcagcc gctggatgcg
     1021 ttgaaactgg gcgcggccgg gcggtttgcg gaacacctgg ccggtttgca gaaagccgat
     1081 cagccgatcg ccgagccgca ccgggacggc ggtctcgctc aggccggcgg ggcaggtgag
     1141 cgtatgcgcc tgctgggccg cggcggcgga cagttgttcc tgcaccgaca agcaagacgc
     1201 gcaggcgcgg ctggtctcca gcaccacggc gcaaaagggg ctttcgttgc gctgcccatg
     1261 atgggggagc tgccaggact ccaccgcccg cagcgccacc ggcagcccgg tcgcctcggt
     1321 gaaggcgcgc gcataatcct gaaacaccac ggaattgttc agcgcctcga ccaggctgcg
     1381 gtcgtcgttc atctcgctgc ttccgcgcgc aagagccggg gactcccggg ccggagtttt
     1441 ccggggggct tgcggacggg tttggccggg cgcacgcaga gccgttcccc cgctgtcttc
     1501 cgggcctggc tggccaaatg ttgttcgcat ggcaacaaat taaggcaacc gaagggccga
     1561 gtcaatcccc gcggtccgtg aaacagcttg ccagacggcg ttcaaaccgg ttcagtccct
     1621 gtcttttgaa caggcatgaa agcgcggcat ttggtttttc tggtgctgat gaactgctgc
     1681 tgggccggag tttactcggc ctataaagtc attggccaga gcctgccggg aggcagcatt
     1741 atcacgctgc gcttcgcgct ggccgccggg tgcatgctcc tgatctggcc gtggctgcaa
     1801 gggccggccc cgcgcgggcg cgatcttttc tacgcctcgc tcatgggggt gctggacttt
     1861 gtgatcgccc agcgcctcca gatttatgcc aacgccatcg gttccgccgg caattcctgc
     1921 gtgctcatgg cggcccagcc gctgtttacg tccgttgcgg cggcgctttt tttgcgggag
     1981 cacattggcc cgcggcgcct gggcggtttt gggctaagca tcctgggcgt cataattctc
     2041 aatcgcgtct ggcgcagcga ctttcaatgg gccgggctgc tcccgagctt gattttcatc
     2101 tccacgttct tttgtgagac ggcctactcg atcctcggca agcccatagt cacccgcgcc
     2161 agcggggcca agaccgtcgc ggtgtccttg ctggccgcca cggccaccaa tctgctcatt
     2221 gacggtcccc ggaccttgag cgccgcgaag tcactgccgc cccatgtatg gtggctattg
     2281 ctggggctgg ccgttatttg caccgtcgtt ggctatacgc tctggctggt ggtcatccgc
     2341 caatgcccgg tcaatgtcgc ggtcctcacc gttttcgttc aacccatctg tggcgtagcc
     2401 ctggccgcgt ggtggctggg cgagccggct cactggggcc atatccttgg cagccttacg
     2461 attctggcgg gactgattct ggggctatcc cggcaaattc accgctgaac cccgcgcaac
     2521 cgccgcggtt tcgcagtgtc cgcatgattg acagggcgga agaaatccgg caggctttcg
     2581 ccctggtcat tagccgttaa agatatgaca accctggaat caaccgcccg ccggaacgaa
     2641 cccccccggg ttgaaggact cggggcagag caacgcccgg caaaaggcgc ttccctccgc
     2701 cccatcggga tggtccgggg agcggtttgt ttcctcatga tttttggggc ggggtgtttc
     2761 ggcccgcccg cactgtctca cggcgccacg ggcgattccg gcgcttatac gaacccggtg
     2821 tatgcggcgt ccatgcccga cccttccgtc atccggcacg aggggattta ttacgcgttc
     2881 ggcacgacgg gcaacagccg cacgccggac ggccgcattt tcaccgtcct ccggtcgcgg
     2941 aacctggtgg aatgggaggc gttggggggg gcgttgaagc cgccgtcgcc gaatcgccag
     3001 gttcaatact gggcgcccga ggtggttttt caccgggata ctttttatct ctattactcg
     3061 atgggcgggc ttgagcccga gaagttcgag ctgcgggtgg ccaccagttc ccggccgcaa
     3121 ggcccttacg aggacgcggg ccgccgcctg ctggattgcg agaccaaccg cttcaccatt
     3181 gatgcctttc cttttcgtga tgacgacggg caatggtatc ttttttatgc gcggaacttc
     3241 acgaattcgg cgcccggcca gcatcccggc accgcgctgg tggtggatcg gctgctggat
     3301 atgacgcgcc tggcgggcga ctgccgcgtg gtggtgcgcg cccgctatga ttggacgctt
     3361 ttcgaggccc accgccgcat ggatgtttat ggcgcgacgt tcgactggca tacgattgag
     3421 gggccgtgcg tggtgaagca cgcgggccgc tactactgtt tttacagcgg cgcgaactac
     3481 caaacggacc ggtatggggt ggattacgtg gtggccgacc atcccctcgg gccgtacacc
     3541 ggccagggcg atcgggcccg cgtgttgcgc agtgtgccgg gccacgtccg ggggccgggc
     3601 caccattcca ttgtgccggg gccgcacggc caatcccagt acctggtcta tcacgcctgg
     3661 gatccggcca tgcgcgtgcg ctggatgtgc ctggatcaac tgctctggac gcccgagggg
     3721 ccgcgcggcg cggggccgag ctggacgcca caaccggccc cccgatgagg actgccatgc
     3781 aacgaaaacg gatgcggtat ttatgggtag ccgggctgct gctgggcttg ggaactcacg
     3841 ccccggccga ggccccgggc gacgcgcttg cgcgcggctt tgcggatccg ccgatcagcg
     3901 cccgcctgcg cgcgtattgg tggtggctca atggcaacgt gaccaaggcg gccatcaccc
     3961 gcgacctgga ggaaatgaag gcccagggtt tcggcggcgc gctgatttgc gatgccggcg
     4021 gcgcgcagca ggacggcaat gatcccgtgc cgcacggtcc cacgttcttt acgcccgcgt
     4081 ggcgcgagct ttacaagcat acgctgcggg aagccgaccg gctggggctg gagatgagcc
     4141 tgaatatcca gagcggctgg aatctgggcg ggccaatggt ccgggcggaa gacgccgcca
     4201 agaagctgac ctggagcgaa gcccggctca cggggccggc ccagtacgcc caggccctgc
     4261 cggccccgaa agcgcgggac catttctacc gggacattgc cgtggtggcc taccgccttc
     4321 cgccgcaaga cgggccggcc agcgaggggc aggggacggc ccgcccgccc gcccgccgcc
     4381 cgcttcaaaa ctgggagcag aaggcgctgc accgtccgct gcatttttcc gcgccggata
     4441 cggcgccgtt gttcgcggag gaaaaagacg tgccgggcga ggaggacgcg caggcccggc
     4501 aggtgctcga tctgacccgg aatctctcgc cggaaggcgt gctgcgctgg gaggtgccgg
     4561 ccggccactg gcaggtgctg cggctgggct gcaccattgg cgaccactcg cgcgtctcga
     4621 cctgcagcga tggctgggag ggctatgccg tggatgtgct ggatgcgggc gccttcaacc
     4681 gctattggga cgcggtcgtc gagccgctga tcgccgacgc gggccccctg gccgggcggg
     4741 tgctcaaata cctgcatacg gacagttggg aagtcgagcc gctgaactgg ggccccacgc
     4801 tgctggcgga gtttcggaag cgccggggct acgatccgct gccttacctg cccgtgctga
     4861 tggggcggat catagattcg cgcgcggcca gcaatcgctt cctttacgat ttccgcaaga
     4921 ccgtgggcga actggccatt gataatcatt accgcatctt tcgcgcccgc gcgcaccggc
     4981 acggcctgct aatccatccc gagtccggcg gcccgcacgc ggtgcccata gacgcccagc
     5041 gatgcctggg catgaacgac gcgccgatgt ccgagttctg ggcctggtcg tggcggcacc
     5101 gggtggggga ccacaatcgc tttttcgtga agcagccggc ctcggcggcg catacgcatg
     5161 gccatcgcct cgtgctggcc gagggcttca ccaccattgg cccccattgg caggagacgc
     5221 tctgggacaa cctgaagcca tccttcgacc gcgcgctttg cgaagggctg aacctgctgg
     5281 tatggcacgc gtttgtctgt tcgccggcgg aaatggggct gcccgggcag cagtattttg
     5341 cgggcgcgca ctttaatccg aacaccacgt ggtggacgaa gtccggccct tttctggcgt
     5401 atctgaatcg ctgccagttt ctgctccagc agggccggtt cgtggcggat gcctgttatt
     5461 attacggcga tcacgtgccg aacttcacgc agctcaagcg cacggatccc gcgcgcgtgc
     5521 tgcccggcta cgattatgat gtcgtgacgg aggaggtcat tctccggcgc atgacggtgc
     5581 gcgaggggcg cctcgttttg cccgacggca tgagctaccg ggtgctggtg ttgccggagc
     5641 ggcccggcct ttcgctggcc gtgctgcgga aactgcggga gctggtccag gccggggcga
     5701 cggtcatcgg ccccaagcct tcccagactt actcgctgag cgggttcccg aaaagcgatg
     5761 cggccgtccg gaagatagcc gatgagttgt gggcggacga agcgcagggg cggcgcggcc
     5821 agcgtttgcg cgggcgcgtg atttccggcc agacggcccg cgccgtgctg gcggcggatg
     5881 gcatcccgcc ggacttcgag tgccgcctcc aggcgccgcc gccgcccgcc gcaatcccga
     5941 acgcgaacct gggagcggcc gggctggccg atgcgtgggg cgatccccgg acggcggcag
     6001 ccgggagcgg ctccggttcc ttgccgccgc tggattatat ccatcgctcg accggagagg
     6061 cggagatcta cttcgtggcc agccggtcga atgcctggct gagggcggat tgctctttcc
     6121 gcgtcgtggg caaggcgccg gaaatctggg acccgatcca tgggacgacg cgtccggctg
     6181 ccgcctggtc atctcaggga agccaaacca ccctgccgct tgagtttgcg ccgtatggct
     6241 cgatgtttat cgtgtttcgc cagccccagg gcgggggcaa tttgccggcc ccgcgccgga
     6301 attttccggc ctgcggcgtg gcgcatgagc tgggcggtcc ctggacggtc aagtttgatc
     6361 cccagtgggg cggcccgtct tcggtcgagt tcgagcgcct ggtcagttgg ccgcagcggc
     6421 cggaggaggg catcaagtat tactcgggca ccgccactta cttcaagacg ttcgatctgc
     6481 cggaatcatt gcgccgtccg ggcctgcgcc tggcgctgga tttggggcag gtcgccaacc
     6541 tggccgaagt gcgcctgaat gggaagaacc tggggattgt gtgggcgatg cctttccggc
     6601 tggaagtgac ggacgcgctc aaaccggagg gaaaccagct ggaggtggag gtcgtcaact
     6661 tctggcccaa ccgcatcatc ggggatcagt ttctggcccc ggagaagcgg ttcacgcgca
     6721 ccaacatccg gaaacttacc aaagacaccc cgctgctgga gtcagggctg ctcgggccgg
     6781 tgcgcattct ggtgcaagag cactgatgcc ggcgcgccgg atgggcttcg ggcgggagcg
     6841 gccccagggt tgcgccgcca cccccgccgc ccgcagaaaa cacccgccgc caggttaaga
     6901 ggcggcggca agcaattgat tcagccgggc caggacggca aaggcatcgg ccacatacaa
     6961 tacgtccgcc ttgccgcggg cccagccaca gttggggtcc aggctgatgg cgcggcgttc
     7021 attgatgaag cgccagccga cggtgtgcgg ctcctccccg tggcagcaag ttgccaggcc
     7081 cttggggtgg cggggcgtgg agcctgtctg gccgatctgg ttcaggtgcg tcaggaagga
     7141 gagcaccgcc tggccttccc cgcccaggtc caccagcgat ttgctgctgc cgagggacgc
     7201 gcgcgccagt ttaaggcagc cgaggatcaa ttcttccgcc tcttttacgt gggtttggcc
     7261 gtcggcctgc tttttggtcc agccggcgcc agcgaccaaa agcagatcgg catcgggccg
     7321 gatggtttgg gcatcggcgg cgagctcgcg aacaccgacc acggtagtgc gctggcaggc
     7381 ctcggacagg gttacgggga ggatccggac gctggcggtt gccgcggctc cttgccaggc
     7441 ggattggctg ccgggatcca gcaggataaa ccacggacgg tgcgtgcgct gcagggacgc
     7501 ttccatccgc tgccgataat accagcgatt gatagtgatc gcgcccgagg cggcggaaag
     7561 gccggtgacg tgcgtgtccg cgcgtccgcc caaccgctgc gcgacgccgg gcagaacccg
     7621 gttccagcgg gcggtagccg gggcgataat gagagtggcc tgggcggctt tgacgagagc
     7681 ttcggccgcc gccgcatcgc tcgcataacg gggcgaggca aaatcagcgc cggcgacgcc
     7741 caggaaagcg tccgcgccgc aagcgccaat ctgatcagcg ccggattgca cggtttgccc
     7801 cacgagcccc acggccagct tggcctccgg caacccagcc ctcaaggctt tggccgcacc
     7861 cagggcttcg aacgccggct ttgccagcga gccgtcggct tcggtgttga ttagcagcag
     7921 aatcgtttcc atgagagaac cagggggtta gcgttttatc cactcgaaga tttcccgcgc
     7981 aatttcgtcc ggagacttgt ctttcacaat ccgggtttca cgcaactgct tcggcagcgc
     8041 aacgctggcg aaggatacgc ccgcggcggc cagtttaacc ggtttggctt tttgcaacgc
     8101 gggcatcacc gtgcgcatat tggccatgcc cacttgcggg ttgttccggg gttcgggcaa
     8161 attgcccgtg gcccagccga gcaaggccgg agggccggcg cagcgggaga cctgctgttt
     8221 gccgccctcc acccgctcga ggatttccaa cgcgccgccg ggcagcactt tcaattcgtc
     8281 cacgccctgg aattggtcga ggatgcccaa tcgctcggcc accatttgca tcgtcacccc
     8341 cgccccgcgc gaagcggatt cccagccgcc aaacaagagc aatttcgacc ggtcaagccc
     8401 cgggatggcg gcaatcgcct ccgccaggac agtggcaact tccgccgctt cggcaaagcc
     8461 gctcgccggg ccgtccaggg cgatcagctc gaaaggcacc ttttgggcga ccgtcatcat
     8521 gacctgctgc aatttggctt tggggcccag gctcaccagc cagactttgc tgccgggtgt
     8581 atttttggcc agatgcgccg cttcatacag cgcatggctt gcccagggat ccaagacggc
     8641 ggggagcatc atttcattgc gcagggatgg gccgtcgggg ccgctgaccg gttccagcgt
     8701 ctggagcggg tccggcacta tgctgccgca aacaacaatc tgatattcat tgctcatgcg
     8761 acgcagataa tcctatttcg cgcgcggttt ggaaagccag aaacaaggat gccagcgtcc
     8821 gagtcaataa acgggagcgt tcatgtccgg cgttcctcgt tgtttttgat cggcggtcca
     8881 gcgcgctcat cgcccagccg gtgaccgtgc ctccgcggtg cgcggggttt cccggcccaa
     8941 tatcgttaac gcaatcgtag gtttggcagc agcaaggagg gtgcaacgga accgcagata
     9001 ggaaagagag gttgagactg aaacttgatg agaaccgctc ctccacaaga accgtgacgt
     9061 aactccggca gagcgtcagc cgggaatctt tggtctcaag caggggcaat tcccgcgcca
     9121 aacttaggga aaaggccggt tgagaaacaa caaggggcgg ctcattgttt tgaacgagcc
     9181 gcccctgctt taaccagcca ctatgaaaca caggccgggc atccacccag attcccgacc
     9241 cactgcagtt cctgttgaac tgtatagatg agacagagat gttgtgatct ttgttcaaac
     9301 ctttgccgaa aattttcgcg acccgccccg gcccggcatc gggaatgacg gcagccgggc
     9361 ggcggaaagg gattgatttg cgcggagatt gtggcaaagt accgggaaac gatgtgccac
     9421 atgaaacagt tcccatattt gcgcgagcgc ggtaaaaagg tggttgggtt tggtttgctg
     9481 ttggcgctgg ggtgtctgcc ctggaccggc gcgctggccg gcccgccgac cgtgcaggaa
     9541 ctggtggacc agttcagcat ggccaattat tgcaacgtcg tcagcaataa gctctatacc
     9601 cgcaagggga tgaaccgcgc tccggtgcag gtcggcgggg cgcaacacga tctctgccgc
     9661 gacgcgatct acgacgagtt tcagcaggtc ggtctgaacc cgtacaagga tcccttttcg
     9721 tacgtggata ccatggggcc gaccaccgtc catgtctgca acatcatcgc cgtgaaggag
     9781 ggggttcaga acccgaacaa cgaagtctat gttctgggcg cccactacga ttcgcgggag
     9841 aatccgggtg ccgatgacaa tgccacgggc gtcgggtgcg cgttggaaat cgcccgcatt
     9901 ttcgcccagc atcactttgc caaaaccatc gtcttcgcca ttttcgacag cgaagagcgc
     9961 accgaggcgg tcaccggccg gcaccggctg ggcagcctgc gatatgtggg ccagcacctt
    10021 aacgacaata tcaagggcat gatttcggtg gatatgatcg gctggcaggc gcccagcccc
    10081 aatcgcaata aggcgtatat ctgcgggcgc cccgccttca acacgatccg ttacgaactg
    10141 caggcggcgt tgacgaatta cggcggcctg aatggcgtaa tctccacttc cgacaacatg
    10201 agcgatcact attccttcga gcaagccggc tttagcgcct gctgcctgat cgagtataac
    10261 tggtcgggca actcgcgcta ccatacggcc aacgattacg tggaagcccc gggctacctc
    10321 gactgggact acctggagaa gatgtgcaag ggcacgattg gctactttgc cgcgcagctt
    10381 caaccggtgg atgtgacccc gcaggtgctt tccgttcagt tcgacgcggg cggctgtatc
    10441 atccacggcc agggcctggc gctttgccaa tacgccattg atcgctgcac cgatctcatc
    10501 accccggatt gggtggcgat tggcaccaac accgcggccg ccgtggacgg cacttttgcc
    10561 gtgctggatc cgggcgcgaa ccctaacagc cagccggccg gattctaccg ggcgcgcttc
    10621 gtctccggct acaccggcgg cccgggcaat ctgccgcaaa tcaccgtgca gcccctgcat
    10681 cgcgttgtgg acaccggcgg ggcgaccagt ttctcggttt cggccatcgg cgatccgccc
    10741 ctcaactacc agtggcggct gaatggcaat gcgattgccg gtgccaccaa taatacctgc
    10801 gtcattctgg acgcccaggc cgccgctgcc ggtgattatt cggtggtcgt cgccaactac
    10861 tccggcagcg tgaccagcaa ggtcgtttcg ctgacggtgt atcccccgca gacccaggtt
    10921 tttgcggatg acttcgactc cgacacctcg gccaactgga cgctgtccca gagctcgacc
    10981 gatacccggt gcactttcaa ctacgattac tccgccgacg gcattccttc cgcccccaac
    11041 tcggtgggag gcacgacccg cggggtgaaa ttcgaggcca atatgaccaa aggcatcaca
    11101 gcggcgttgt ccatgtcgcc ggtggggctg agcgttggcg gggattaccg cttgtgtttt
    11161 gatatgtgga tcaatgccaa cgggccgttc ccgctgggag gcaacgggtc gtcggagtat
    11221 atcaccggcg gcctggggac gacgggaggg catgtccagt gggaccgggc gggcagcgcg
    11281 accgatgggt attggttcgc ggtgaatggc gaaggacagg ccgtggacac gtccgtcatt
    11341 tcggactacg gtgcttacgc ccgcaccaac gtccagacgg ccgccagcgg ggtttacgcc
    11401 gccggcacca acaataattc gcgcggtaac ctcaatggat attacgtcac cgctttcccc
    11461 ggccaggctc cgccgctgct gcaacaggcc acctatcccc aacagaccgg cgtgctggag
    11521 gatggcgccg ttggcttcgc ctggcgcgag gtcatcatcg ccaaacgcgg caacatcgtg
    11581 gaatggtcta ttgatggggt aaagctggcc gccttcacca gcgcctccgt ggttgccagc
    11641 aacatctttg tcggctactg ggaccccttt gtgtccatct ccgacaacgc ggcgctgagc
    11701 ttcggcctgg tggataacgt gcgggtggaa tgggccacca acagcacgcc gcccacggat
    11761 gtgattatca tcgacaaccc agcggccacg gtggcaggcc aatggactaa cggggtcatg
    11821 tccacggaca agtatggcga cgattacttt tatgcctttg ccgggacggg tacccagtac
    11881 gtggaatatc ggccgaacct tccggccagc ggcaactacg cggtatatga atggcatccc
    11941 cagggcgcca accgcacgac cgatgccccc atcgccatca agtatcacgg cggttcgcag
    12001 acggtaagga tcaatcagca gatcaacgga gggcagtgga atctgctggg caccttcaat
    12061 ttcgccgcgg ggacggccgg ctacgttcgc atccaggaca actttaccaa gggcacggtc
    12121 gtaatggccg acgccatcaa gtttgtctat gcgccctgag cgccggggcg cgccgcccgg
    12181 gcgcgtgggc agtcctacag gccgccaaac cgccggtggc ggcggtggta ttcctccagc
    12241 gccgcataga actgggggcg gcggaaatcc ggccacaggg ttggcgtgac caccagttcc
    12301 gtgtaggaaa tctgccacag cagaaagttg ctgacgcgca tctccccgct ggtgcggatc
    12361 aacaggtccg gatcggggat attgcgcgtg taaagatgcc gggcgaagag ctgctcggtg
    12421 atgcccgccg ggtcgagttc gccggctttt accttttggg cgatgctgcg cgccgcgtcc
    12481 acgatctcgg tgcgcccgcc gtagctcaag gccatgatga gcgtcaggcc gttgtttttt
    12541 gccagggtgg cgatggattt ttgcaggtgg ctctgcacgc gggctggcaa ccggtcaacc
    12601 tggccaataa tctggagctg cacgttgttc tggtccagct cgggagtctc ggttttgaga
    12661 tagtgcacca ggtatttcat cagcgcgtcc acctcatcct tcggccggtt ccagttctcc
    12721 gcggaaaacg cgtaaagcgt caggtacttg attccgagct cgccggcggt ccggatgatg
    12781 acccgcgccg attcggcccc cgcgcgatgc ccctcgatgc gcggcaggcg ccgctgccgg
    12841 gcccagcggc cattgccgtc catgatgaca gccacatgcc gcggcaacgc ggccctggcc
    12901 gcgggactca gatgcgccgc cggggtgctc atcgaaccgt tcgcgtcctc aaagcatgat
    12961 tgtctgctcc cggcccggcc ccaccgaggc gatgatcagt tgcgcgccgc tcagttccgc
    13021 cagcgccttg aggtaggagc gggccttggc gggcaggtct ttccagcggc gcgcccggtg
    13081 cgtcggcgtg cgccagccgg ggaactcggc ataaatgggg gtgcagcgcg ccagcgcctc
    13141 gatgtcgttc ggcacgtaat cataacggcg atcgccgtcg cggtagccga tgcagacctt
    13201 gatggtctcc agcgagtcca gcccgtctat attggtcacc gccaggtggt caatgccatt
    13261 gaccaggcag gcgtggcggg tgacgaccgc gtcgaaccag ccgcagcggc gcggccgccc
    13321 ggtggtcgcg ccaaactccc gacccagcgc gtggagcagg tcggcaatct cggcgttctc
    13381 ggtgggcagc ggcccctcgc cgacgcgcgt ggtgtaggcc ttcatcactc cgaccacccg
    13441 gtccatgcgg tgcggcggga cgcccgagcc ggtgcacgcg ccgcccgccg tggtgttcga
    13501 ggaggtcacg aaaggatagg tgccgtggtc caggtcgaga aaagtgcctt gcgcgccctc
    13561 gaagagaatg tccgcgtcgc ggcgcagggc ctcctgcagc aggatgacgg tgttggcgac
    13621 gaacggtttg aggtaatcgc ccgccgcgcg gtactggcga tgaacccgct cgaaggagag
    13681 cggccgggcg ccgtaggccc gcagcacgtc attgttttcc ttgatccgga gccgcagctg
    13741 ctgccggaag cgttccgggt tgaccaggtc aatcacgcgc agcccggtgc gggccgcctt
    13801 atcgccataa gccgggccga tgccgcgctt ggtcgtgccg atccggttct tgcctttgag
    13861 ccgttcgcgc tgcgcgtcca gctcgcggtg atagggcaga accaggtgcg ccgtctcgct
    13921 caggtaaaga ttcccgtggg ggcggatgcc cagctggagc agcccctcga tctcctcgac
    13981 caggctcacc gggtccacca ccaccccgtt gccaatcatg cagaccttct tcctgtgcag
    14041 aatccccgac ggcaccaaat gcaggatgta cttctgcccc ttaatgtgca ccgtgtgccc
    14101 ggcattgttg ccgccctggg tgcgaactac cacgtccgcc tggtcggtca gcacatcaat
    14161 aatcttgcct ttgccttcgt cgccccattg ggcccccacc agaatcgtat tggccataac
    14221 ttcaacacac aaataaccgt cccgccgcgg aaagagccgc gcccgggtca agcgcacaac
    14281 aaaaaccccg aaacgcaagt tcggggacaa gaacactcgg ctcttgcgca aggagactag
    14341 gagtggcgcg cgctcgtgtc aatccccgat cgtgtcaatg ccggatagtg ccgcattgcg
    14401 tttcccgcgg ccctgtgaaa cactggcccc cggtgaaaca atgcatcgta accctggata
    14461 tggagggagt gttgactccc gagatttgga ttgccgtcgc cgaaaagacg ggcattaaag
    14521 agctgcgccg caccacgcgc gacgagccgg actacgataa gctcatgcgc gggcggctca
    14581 acatcctcga tcagcatggc ctgaagctct ccgacatcca gcgggtcatc ggcacgctga
    14641 gcccgctgcc cggggggagg gagttcctcg acgagctccg ctccctggtt caagtcatca
    14701 tcctctccga cacgttcgag cagttcgccg cgccgctgct gcgccagctc ggctggccca
    14761 cgctgctctg ccaccggctg gtggtcgagc aggaccggat tgtggactat aaactccgcg
    14821 tgcccgagca gaagcaaaag gccgtcgccg ccttcaagct cttgaattat tacgtgatcg
    14881 cgggcggcga ttccttcaat gacaccgcca tgctctgcga ggccaacgcg ggctttctgt
    14941 tccgcgcccc ggacaacgtg aagcagcagt tcccccagtt ccccgcggtc gagacctacg
    15001 ctgatttcct ggggctgatt cagcgggcga tgaactgaag cccgcccgcc agcggcgaga
    15061 tggcctaaac tttggggggc gcgtccggtg gcggcggcgc ccgccggcca atccggttct
    15121 cggtgccgtt gagcagccgc tgaatatttc ccttgtgttt gtagatggcc agcacggcca
    15181 gcgcggaaat gactaccgtc agcgttttgc tttccccgcg ccatcccgcg gcaaagggca
    15241 gggcgatggc ggcgcaaatg gacgcgagcg aaacgtagcg gctgagcgca aacaccacga
    15301 tccagacccc cagcgcgatg agcaaggcca tcggcaccag cgcgagcagc acgcccgccg
    15361 atgtcgcaat gcccttgccg cccttgaaac ggagccagca agtgtagtta tggcccagga
    15421 tggccgccac tcccgccccg agccgaaacc actccagcgt ttgccctccg gcggcggcgc
    15481 cgcacgcgcc ccaaagcagt tgggccacga aaaacaccgc cgcccagccc ttggccgcgt
    15541 cggccagcag cacgaaaacg cccgccggcg tgcccaggat gcgaaacacg ttcgtggctc
    15601 cgatattgcc gctgcccaca gtccgaatat ccaccccccg cgccttcgcc accaggtagc
    15661 ccgtcgggat cgagcccagc aaataggcga tcagcaaggt cagcatgtaa ccagcccaaa
    15721 acatcgcggc actctagagc cccggccccc gaacttcaaa gttcaaatga gccgcccggc
    15781 cggcgtggct ccggattcgg cgctcaattc ccggccgcgg ccggctttcc cgccccgcgc
    15841 cgatgcccgc ttcggccccg gttttagcgg ggcaaaagca gggcggacgc ccgctttgcg
    15901 ccttttaagc tgagttgcca gttgtcagcc gcctccggtc aatggcacat tggcgggcat
    15961 gaatgcggaa catgagccgc cggagacagg cccggtgagg gttgcggtgc tgggggttgg
    16021 ctccctcggc aaagagcatg cgcggattta cgcggaactg gcggcggcgg gacaaatcga
    16081 gttcgccggc gtgtatgaca tcgccgacga gaccgcgcag cgcatcgccc gcaagcacgc
    16141 ggtgcggacg tttggctccg tcgccgaggc ggcggccgcc agcgacgccc tgagcgtcgt
    16201 gacgcccacc accacccact ttgcgctggc gcaagagctc ctccggcagg gccggcatgt
    16261 gctgctggaa aagccgatga cggacaacgc cgcccaggcc gccgaactgg tccagttggc
    16321 gcaacaaaag ggctgcgtgt tgcaggtcgg ccacgtcgag cgcttcaacc cggtctttca
    16381 gtatctcgaa accgtggcca ccgacccgcg gttcatcgag gcccaccgcc tctcgccata
    16441 cccggcccgc agcactgacg tcggcgtggt gctcgacctc atgattcacg acctggatgt
    16501 cgtgctggcg ttcgtcaaat cgcccgtgca aagcgtggac gccgtcggca tcccggtgct
    16561 cagcccgtcg gaggacatcg ccaatgcgcg cctgcgcttc gccaacggct gcgtcgccaa
    16621 tctcaccgcc agccgggtca gcccggagcg gatgcgcaag attcgcgtct tcagcggggg
    16681 cgccccggcc agctatatct cgttggatta ccgggcgcag gaaggctaca tctaccgcct
    16741 ggcgcgcaat gacgaggagg aaagctccct cctcaaaaag ctgctccacg cccgggatgc
    16801 cgccattgtc agcgagttcg ccggccagcg catcgtgcgc gagccggtgc cgctggcccg
    16861 ggaggagccg ctgcggctgg aattgcagca tttcattcaa tgcgtgcgcg cgcatcgcac
    16921 cccgcgggtc agcggcgaat ccgccaagcg ggccctggat ctggccctcg agattacccg
    16981 gcaggttcaa accgccgggc ttgggcaaaa ccggtaagac gctggcgcgg cggcgatgag
    17041 aaccttatgg cagcattgaa cgaccgagca ggacttcctt tccgtcccgc gcacccgcgg
    17101 gaaaagctga ggtgaagaca tgaggcccgg gagtttcatg ctgatcgcgg gcgaagccag
    17161 cggcgacgcg ctggcggcgg aactggtgca cgcccttcgc caggagtacg ccgaggctcc
    17221 cgccgttccc accaccgatt atcaaccgcg cctgaccacc ctggccccgc gcttcttcgg
    17281 cgcgggcggc ccgcaaatgg ccgccgcggg cgtggagctc gatttcgata tgacggcgca
    17341 ctcggtgatc ggcctgtccg atgcgcttcg gcattacggc aaattccgcc ggctcttccg
    17401 gcggctgctc cagctcgcgc gcgcccgcca gccggaggtc atcgtttgcg tggatttctc
    17461 cggcttcaac cgccgctttg cccgggccat ccggcagggc acccgtccgc gcgccgacgg
    17521 gttccataac tggaagccgc ggatcatcca atacgtctcg ccccaggttt gggcttcgcg
    17581 cgccggccgg gcccgccaaa tggcgcgcga ttacaacctg gtgctcagca tttacccctt
    17641 cgagaaggac tggtatgcgc agcgcgtgcc gcaactgccg gtggaattcg tcggccatcc
    17701 cctcgtggac cgctacgggc cggaccccgc ggcccaggac gaaagccgcg cgcgggaagg
    17761 cgggccgttg ctgctgatgc tgccggggag ccgtgccgcc gagctccggc gccacttgcc
    17821 ggtcatgctg agcgccctgg acctgattcg ggccaaggtg gtggggctgc gcgcccggat
    17881 ggttctgccc cacgacgcgc tcgcccagca ggcccgggcc ctggggctgc cccgcaacct
    17941 ggaattgcgc atcggcggtt tgtcccgggc gctggcggag gccgacgtgg cgctggcctc
    18001 caccggcacg gtcacgaccg aatgcgctta ctttggggtg ccgaccgtgg cgctctataa
    18061 gacctcctgg agcacctggc aaattgggcg gcgcatcgta acggtaaaat acctggcgat
    18121 gccgaacctt ctggcggggg aggaagtctt ccccgagttc atccaatccg cggctaatgc
    18181 cggcaacctc gccggggccg tagtcgcctt gctgcgcgat gaagcccgcc gcgcccggat
    18241 caaagcccgg ctgcgcgaag tggccgccac gctgggccct cccggcgccg cccgccgcgc
    18301 tgcccaagcc atcctggcaa ccttccaggg acgcccgtaa ttcccacggt cgcggttcct
    18361 tctcccgcct tgagaccggg gatgcctttg taatccggcc agacaagaac ccggcccagc
    18421 ccggtccggg cgctccgctg aaggcatttc gcccccccgg ccagtcggtt tcaaggcgat
    18481 agccccccag cagccgtagt ttcccacccc tcaagcaggg ccagaatacg gctctcaata
    18541 ggcttctgac aggccgtgta caaggggtgt acaagggatg ctcaccgaat gcacatggga
    18601 ttttagcttg ttcacccggt gggcatccct tgtacacccc ttgtacacgg cctgtcagga
    18661 gctattggaa agctggataa aggtttggaa atgcgtggat tgcgatagaa aggcgaggat
    18721 aaagccagat aacgagagct ggcagccaga tagcctctct atgggagtgt ccaataattg
    18781 gacagttcga ggtaaggttg ggagttggcg tcaggcggag gcttgtcgaa gccgccggaa
    18841 acgataacaa tgccggggat gagccgcata ggtcatagat gccggggatg gttgtttcgg
    18901 agggtggcgg cgtgggcggt ggcagtggcg cttgcgttct ttgcccggcc gggtgcggcg
    18961 cataccctgc cgatcagcta cctgcggctg gttccggagg cggattacct gcatttggag
    19021 ttgattttta acccgttcga gctcgccctg atggcggagg tggacgataa cagggatgcg
    19081 gaactggacc tgggcgaatt agcgaggcat gggcaggtgg tggcggatgc cgtggtcggc
    19141 gcgctcaagc tgcgcgcagg cggggcggtg ctgcggccgg agaccgcggg catggacccg
    19201 gacctgagcg ggcaccacgt ccgcctgcgc gctcattaca aagtggacgc gcgcgggctg
    19261 ccgctcaccg tcgaatcgga cctgcccgcc cttaccagcc cctcgcatct cacccaggtg
    19321 acctacgaca acggcaccaa ccggcaactg gcccagtttg attctcattc gcgccgggtg
    19381 actttccagc cgccggcgcc gctcacgtcc gccgttgcgc cggccgtgtc gcgcgggcgg
    19441 gcggccgcca gcgtggcgtt gctggcgctg gttgcgtttc tgctggttat cgcgggcgcg
    19501 gcgctgggtt ggtgcttgcg gaggcggcgc gcccggagat agactggctc tcgccgcgtc
    19561 cgataaacga ccggctcgat cagaactatg actacgaaga catcctgttc cgctggctgg
    19621 tttctcgctt tcaccctgac atttggcgcc gcgacggcgc ccgccgccgc cccgccgccg
    19681 gcgctctgga cgctgggcac accggacggc gcggccatgg aatttgcgcc cggcggccgg
    19741 gaggaactga ccttcacggt cggccagagc gttgtcagca aagattttgc gggcagccag
    19801 gccggggcgg aggattggga cggcaagacg aaagaaaagg cgtatcgcat tgtcttcgac
    19861 cttgcggaag cgccgcaggg gcgttacgag ctggcgctgg atttgattta tcactccggg
    19921 gcgccgacgc agttcaaggt cgcggtcaac gacaagaccg gcattttccc gatccagccg
    19981 gtgggcaaaa agagcgcctg gggcgaggag ggcaacgaaa tgctgctggc ccggcagcac
    20041 ctggtcgtgc cgattgatgg cgcatggctg aaggcgcagg gcaaccaggt ggtcctgacc
    20101 ccgctggggc ttggcagcct gagctatgac gcgatcgcgt tccggcgcgc cgccgccgcg
    20161 cccgatcccg cgccgcggct ggagccgacc attttcttcc accagcgcgc gggccagctc
    20221 aaggaagttt gcaacctggt ggtgccgttc gagcagcgtt tcgaccgcgc cgccgcggtg
    20281 gtgcggctcg gcccacagag cttttccgcc acgctgacga atgccgcgta tgacttcggc
    20341 tggtgggtgg agccgatcga aatcccggcg gcggcggtgg ccgggcgggc ggatctggaa
    20401 gtcgcgctgg ccggccagac ccggcggaac gcgcacgaca tccgggcggc caagcagtgg
    20461 aaggtattcg tctgcccgaa ggtgcataac gatgtcggct ataccgatct gcagccgcac
    20521 gtcaacgagc tggataatcg caatacggac cgggtgctgg acctgctggg ggaatatccc
    20581 ttttacaagt tcaacttcga gacgtcgtgg ctggtggata attacatgga ctgccggacg
    20641 gcgccgtttc agaagaagtt cttcgactac gcccgcgccg accggatcgg catcaatgtg
    20701 ctgtacctga acctgatgac cgggctgtgc acgggcgagg agctttaccg cgcgatgtat
    20761 ttcacgcacc ggctgcaccg cgccgagggc agcgacttcg cgtttgcctg cctcaccgat
    20821 gcgccgtcgc actcctggtt tctgcccacc ctgctgcgcg acgttggggt gaaggcgttt
    20881 tccaacggcg ccaaccagac ccgcgccccg atcctgcatc acagcgacct gaacgaggat
    20941 tcgccctact attgggaggg cctgaatggc gagcgcatct tcatgtggta tgcccgcagc
    21001 tacgtgcagc tcaagcgcct gaccggcccc ggctggggcg gcggcatctc cagcgtggcc
    21061 tacctgaaac gcagcgtgcc gcagtttctc gcccgctatg cgcgcgacaa ttatgcgccg
    21121 gacgcggtga tgatctatgg cgcgtacgtg gataacgccg ccatccccgc gcgcggcgac
    21181 gcgccggcca tcgaacagtg gaaccgggag tttgaatttc ccaagctcat cgtggccacc
    21241 gattcggatt atttcaacta cgtggaagcg catttcgccg accggctgcc ggtgtatcgc
    21301 ggcgactgcg gcgcgtactg ggaggacggc gtcggctcga ccatgcacgc gaccatcaaa
    21361 aaccgcgaga cgcagcgggt tctgccggcc gccgagaccg ccgccgcgct ggcgaccctc
    21421 ttcgattccc gcgaccgcta cccggcgcgg gatttccacc gcgcctggaa caacgtcatg
    21481 ttctacaacg agcatacctg gggcgcgcac aacagcatct cccagcccgg gcgccagttc
    21541 gtggagcgcc agtgggagat caaggagagc tacgccaccc gcgcgaacct cgacgcccgc
    21601 accctcctgg cccgcggctg caaccgcctg gcccagcaaa tcgccgtgga cggcagcagc
    21661 atcctcgcct tcaactggca gaaccgcccg cgcaccgcgc cgctggagat cgagctgggc
    21721 accggcgacc agttggtgga cctcgccgac aaccaggtgg tgccgctgga catctatgcc
    21781 gagcgggacg gctggcgcaa agtgcgtttc gtggcccgcg acgtgccggc gatgggctac
    21841 aaagcctatg gcatccgggg ctggaatccg tccgcgcaca aggagaacga aaaactggcc
    21901 ggcgacacca tcgaaaacga gttttaccgg ctgacggtgg acacccggac tggggggctc
    21961 aagagcctgt acgacaagag cgcccagcgc gagctgcttg atgccgccgc gccttaccag
    22021 ctcaacgagt atctttacgt gagcggcggc gaaggctcgc tcatcctgaa cttcaccttc
    22081 ggctcgccgc cggcgaatct catggttcac cggccggagc cggcgacgat caccaaggtg
    22141 cgcaagacgc cgctggggca gcgcctgatt gtggaaacga agtcgcagaa cacgcccaaa
    22201 gtggtcagcg aatacctgct gtacgatgcc gtcaagcgcg tggagatcgt gaataccgtg
    22261 gagaagcacg aggtgcgggc gaaggaggcg gtgtacttcg cgttcccgtt cgccgcgcgg
    22321 cagccggcgc tggaatacca aatccagaac gggtggtgcc gccccaacga ggaccagatg
    22381 ccgggcgcgt gccgcgaatg gttcacgccg cagaatctcg tccacgtccg ggacggcgat
    22441 ttctccatcg cctggtcaac gctggacgcg ccgctggcga ccttcaccga catcaaccgc
    22501 ggcaaatggc tggcgcatct ccccattcgc aacgggcatg tctattccta tgtgatgaac
    22561 aactactggt tcaccaatta ccgcgcgcag cagggcgggc ggttcgtttt ccgctatgcc
    22621 atcaccagcg ggcgggggct gggacgcgag gaactggccc ggttcgatca ggatacgcgc
    22681 acgccggtgc tggcgtatcc ctttctgtcg tccttctcgg cggcgatctc gcagacggat
    22741 cgccccctgc ccgcgcgcgg cggctccttc ctgacgtgcg acgccgcgaa cctggaaatg
    22801 gtcacgctca aggaagccga ggacggcgag ggcttcatcc tgcgctttcg cgagatcgcc
    22861 gggcgccgcg gcgaggcgca gttcaagctg cccaccttcc acgtccgcga ggcgcatctt
    22921 tgcaacgggg tggaggtgaa ccagcagaag ctggccgtgg cgggcgatgc gctgagcgtc
    22981 ccctataagc cctacgcctt caccaccgtc cgcctcaagc ccgcgaagac cgcggcgaag
    23041 gacgcgggcc ggtagcgcgg cgttttccgc cgctcccgcc gggcgggcgc gccgccgcac
    23101 gcgcccgggt tttcggcgcg gcctcaggga gcgcgaggtg gcggggtcgc gccgtctccc
    23161 ttcaggtgcc ggtcgaagaa cgcttcgacg cgcttcaagt tttgcgcgtt ggtgaagagg
    23221 aagccgccgt gccggccgcc tttgatgacc tcgagggtca cctccgcgcc cgctcgcttc
    23281 aaggcccggg cgaggatttc gctgtgggcg agcttcaccg tctggtcctg gtcgccgtgc
    23341 atgatgagaa acggcgcggc gtctttggat acgtaagtca gcgggctggc ctgccgggct
    23401 ttctccgggt gttgccgggg atcgccgccg aacaattggt ggcgggtgtt ccgggctcgg
    23461 gcggtgagga aaacggtcgg gccataccag tccaccacgg cctgcacgcg gctggattgg
    23521 tcggcgttgc cgcccgggcc ttcgagttcc cggatcccgg cggtggtgcc cagcaacgcc
    23581 acgaggtggc cgcccgccga gccgccccac gcgccgatat gacgggcgtc caggccgtat
    23641 tggcgggcat gggcgcgcag ccagcgcact gccgccttgc aatcgtaaat ctgggccggg
    23701 aaaaccgcat cctggctgaa gcggtagttc atcgaagcca cggcatagcc ccgggtggca
    23761 aactggaaag ccgggccatt ggtcttgtcc ccccgcgtcc agccgccgcc atgcacccag
    23821 aggatcaccg gcaggggccg ggacgccttt tcgggcaggt aaaggtccag ccgattgcgc
    23881 gcgtgcccgc ctttgacata ttccaggtct cgcaggactt gaacgcccgg gagcgcggac
    23941 cgggcgccag ttggcggcgc gggggaagat tgcgcgcacc caaacagcgc cgtgccggcc
    24001 agggccagca gaatcagcaa aaggccgggg aatcgccggt ggggcgtgtt gggaagcctc
    24061 agaagctgcg cggcggcagg catcgtcgtc cggagggtgg gagtttgcgg gcgcatcgtc
    24121 aggaggcgga ttccttcagg tggaacagtt cgaagcgcgg cgccagcagg tgatgcaggg
    24181 caaaggccac caaatagatg cagccgcaaa tgccaaacag gatggcatag ccggcggtga
    24241 tgttgccctg gtgttggaaa tgatccagca accggccggt aagcgcgggg aacataatcc
    24301 cgccggccga gcccgccatc ccgccaatgc cgatcaccga cgccaccgct tttttgggga
    24361 acatgtcgga cacggtcgta aagagattcg ccgaccaggc ctggtgggcc gccccggcca
    24421 gcccgatcag gagcaccgcg gtccagtcgc ccacccgggt caccaataga atgggcagga
    24481 cgcacagcgc gaagatgaac attcccgttt tgcgcgcccg ggtcacggac cagccctgct
    24541 tggtcaggta gccggtgatc cagcccccga agatgctcag cacggtgaca attccgtaga
    24601 tggtgacgat atgaatccag cttttcttga tttccagacc gcgcgtcttt ttgaagaagt
    24661 ccggcagcca gatcaggaaa aaccaccaca cggggtcggt caggaacttg gcgatgaaga
    24721 acgaccatgt ctgccggtag cccagcaggc tgagccagga caccttttcc tgcggccgtt
    24781 ccggctcctg gtcgctccgg atcaattcca gttccgccgc gcccacgcgc ctgcttttgt
    24841 ccggcacatc gtagaacgga atccataaaa agagccagag cagccccacc agacccgcgg
    24901 tgatgaaaca ggattgccag ccgaactggt aggccatcca ggggatgacc gccggagcca
    24961 cgagcgcccc cgcgttggtg ccggcgttga agatgctggt cgcaaacgcc cgctcgcgtt
    25021 tggggaacca caaggccacc gctttaatgg ccgaggggaa gttgccccct tcacccagcc
    25081 ccagcgcggt tcgcgcgcca atgaacccga cgaagctgcc gaccagggca tgcgccgccg
    25141 ccgccacgct ccacgcaatg atggagacgg agtagccgat cttggtgccg aaccggtcca
    25201 caaaccatcc aaagcccagc aggccaatcc cataggcgaa ttggaaggcg gagttgaccc
    25261 agccgaactg ctcgttcgtc cagccgattt gttcgtcgag gatctctttg agcagcgaga
    25321 ggatttgccg gtctatgtaa ttgatggtcg tggcgaaaaa caggagcgca cagaccaccc
    25381 aacggtagcg gccgatgggg gagggcaggg ggacggcaga tgcggattca gatttcccgg
    25441 gttcgattgt cgctggcatg gtgcggacag cttttaccgt ccggctttgc aggtcaagct
    25501 tgcgggtgac ttttcgtgag aaacggcgcg cgccggttgt ttcccggttc gcggttcatt
    25561 gtcttttccc gggcggcccg ttaccctctg gccatgaaga cgttcccggt cggctccgac
    25621 aaagaagccc tgctcagcca gcgaatggcg cagttgggcg tgcgcgaggc ggacctggcg
    25681 gaaactttcg tccggtccag cgggcgcgga ggccagaagg ttaacaagac ggccacctgc
    25741 gttgtcttgc tgcatcgccc gaccggcctg caggtcaagt gccaggccac ccgccagcag
    25801 ggcctcaacc gctttttggc gcggcgctgg ttgctggata aaattgagga gcggcagacg
    25861 ggccgggtgg cggccgagcg cgcccgccgc gagaagatcc gacgccagaa gcgccgccgc
    25921 agccgccggg ctaaagagcg gttgctggcc gacaaagccc atcacgccgc caaaaaggag
    25981 gcccggcgac gggttagcgt ggattagctg ctccggcaaa gcccggtctg aagcttcggg
    26041 cgcgccttcg catagctgcg atttcgggga aagcggcgca cgcgcgcctt gccttgggag
    26101 cgaaggcggg atagcgtagg agccacacat gaatttgact ggcgcatttg cccggtccgt
    26161 cgagcggcag ccgtccaaga tcgccttgtt ctggggcgag agcgagcatt cctacgccca
    26221 actgtggcgc caggcgctgg tggtcgcgga gcaactccgg cagcagtatg gcgtgcggcc
    26281 cggggaccgg gtggggctgt ggcttaagaa ctgcccggag ttcgtcatcg cgttgtttgg
    26341 cattctgcat acgggtgcgg cggcggtgcc catcaataat tttttcaaag ccgaagaagt
    26401 gaactatatc ctgggcgatg cggggattga tgtgctgatt acggatgccg aactggcggc
    26461 gcatcgcccc gcgctggaag cggcccggcc gggtttgaag ctctttcggg tcgaacaact
    26521 cgacgccctg gctgccggtc ggggaggaca aaactccggc tcccgggagg gcggatcagg
    26581 ggcaaaccct tcgccaaagg agagttttcc ttcgccggga gaatcggacc tggcggtcat
    26641 catctacacc tcgggcacca ccggccggcc gaagggcgcg atgctgtcgc acggaaacct
    26701 gctgcataat gtcgagagct gccgcgacgt gctcgccacg gtcgagggcg accgatttgc
    26761 ggtgctgctg ccgctattcc acagctatat gctgacggtg gggctgctgt tgccgctgct
    26821 ggtaggcggc tcaatggtgc tggtcaagtc gctgcatccg gtgcggaatg tcttgcagga
    26881 gattctgcag cggcaggcga cggtgctgcc ggccattccc cagttttatc gcagcatggt
    26941 caacgcgccc atcccggttc cccttccgtt gcggctctgc gtgagcggcg cggccccgtt
    27001 gcccgtgcag gtgctgaaag agtttgaggc ccggttccac atcccgctca ttgagggcta
    27061 tggcctcagt gaagccagcc ccgtggtgac caagaacccc gtggacggca cccgcaagcc
    27121 cgggtctatc ggcccgccca tcccgaacgt ggaagtcagc attcaggacg atgccgggcg
    27181 ctttctggac gcgggacaaa tcggcgaact gtgcgtgcgc gggggtaatg tgatgctggg
    27241 ctattggcgg ctgccggagg aaaccgccaa agtcatgcgc aacggctggc tcctgaccgg
    27301 tgacgttggc taccgggacg aagatggcta ttactacatc actgatcgga agaaagacat
    27361 gctgctggtc aacgggatca atgtctatcc gcgtgagatt gaagaggtgc tttaccagtt
    27421 cccgggcgtc aaagaggcgg cggtcattgg caaacccgat tcgcgcaagg gcgagcagcc
    27481 gattgccttt gtggtgccca atgaaggcga ggttctggcg gaaaaagcct tgctgcaatt
    27541 cgtgcgccgg aaactggctg actacaaggt cccgcgcaaa gtcatatttc tgcccgcctt
    27601 gccccgaaac gcatccggca agattcttaa aacccggctc cgggaactgc cccattgctg
    27661 accctgctcc gggcgcgtct gttggggggc gcgcttttgg ggttgccagc gccgcgcttc
    27721 tattgcatgg tcgccctgca ttgcgaacgg caattttatg agcgcacaac acaatcatcg
    27781 cgattacccc aaactccaca atgccatgtg gcccggcctg gtcggcaaag gcagccccgg
    27841 cgcagagccg ggcattgatc tggataccat gctcgacctg acggcgcggg cggaggtgga
    27901 cggcgtcagg tttgacgggg tggatttgtt tttgagcgat ccgcatgtca gcattgatct
    27961 tgatgatgac ggtctcaagc gcctggccgc caagattcag gcaaagggct tcgtcgtagg
    28021 ctcggtggtg gcgccggtat gggcgcctac tggcggaggt tccgccattg gcagccggga
    28081 ggaacggcag cgtttcgtcg agcagatccg caaagcgtgt ggaatcgccc gaaagcttag
    28141 ccgcctgggg gtccggccct acggggtggt gcgaattgat tccgcctgca gcgtggccga
    28201 ttgggcgaag gatcctgagg ccaaccaaaa gctgctcgcg cagacttttc gagaggcgtg
    28261 cgaggtggcg gaagactatg gcgaacgatt ggccgccgaa ggcgagattt gttggggcgg
    28321 catgcatagc tggacgaaga tgttggacct cctgcggcgg gtggatcgcc ctcgaacact
    28381 gggattccag gccgacatgg cgcacaccct cctctacatt ctcggctaca atgcgccgga
    28441 agacgccatc ctgcctccgg attgggattg gtcctctccg cttcgcctga accaggccat
    28501 caaggagctt accgacgcgc tgcgcccttg gacgattgat ctgcatattg cgcagaacga
    28561 cgccaccgtc cacggttcgg gcacccatga caagaccggg catcactgcc tgcccaatga
    28621 cccgaacggg aagctcgaca ttgttcgcca cgccggctac tggctgcggg gcaaggacgg
    28681 ccgtctcaac aagacgattc ggcacctttg ctgggacggg tgcatgttcc ccaacgcggt
    28741 gatgatgagg cccgagacct ggaatgacat cctgcgcacc atgatcgccg tgcgtgacgc
    28801 ttacggctgg cgcgaggaag aaaaggcggc ggttgaatct ccagcgcctg caatgtcggt
    28861 gcggccggcc aaatccgcca ggccggcggt tgctaaaacc cgtgcgtcgg gcgccggcca
    28921 gcgcgaaaag aaacctgcga aacagcgaat ccgcaaaagc cggacgaagc cgacgcgcgc
    28981 gagagcagcc gccaaagccg ggaaacgaaa agcaaaggcc gttcggaaaa ccaccgcaaa
    29041 gcgggcccca gccaggaaga aggccaggcg gaagaagtag tccgggatcg cgattggggc
    29101 gaagccatat tgctaccctg acatgaagcg tttaatgctc atgctgctgg tcgcggcaac
    29161 cacgatggcc gtggccgctc ccgtgaacca gcttgcgcgg cgggaaaaag ccgcgggttg
    29221 gcggctgctc tttgacggta aaactacgca cggctggcgc agcttcaaaa agccatcttt
    29281 ccccgaccaa ggctggcggg ttgaagatgg ctggctgcac tgcctgggca aaggcggcgg
    29341 cgatattatt acggacgcgc aattcgatga ttttgagttg cagtgggaat ggaaactggc
    29401 tgcgggcgcc aacagcggcg ttaagtattt cgtcctggaa agccgcaacg aagtgcttgg
    29461 gcatgaatac cagatgattg atgacgcacg gccgtcggga gttgatcgcg ggaaccgcaa
    29521 acaacttacc gccgcattct acgccgtgct ggagcccgtc tgcccgccgg tcaagccggc
    29581 tggcgaaatc aattcttcac ggatccgcgt tcagggcaac cacgtcgagc actggctcaa
    29641 cggggtcaag gtgctggaat acgaatgcgg gagtgaggag gtcagaaaag ccgtcgcggc
    29701 gagcaagttc aagaacaagg ccggctttgg cgacaaggtg cgagggcacc tgctgctcca
    29761 ggatcataac agccaggtct ggtatcggaa cctgaaggtc cgcgacctgg ccaatacgcg
    29821 gtaaagcttc tgtcggtcgc ccggagttcc ccgctcaggt ggtaaagggg atcacctggg
    29881 gccggaccag tcgctcgaag tccttatacg ccagtttgat gacctcggtg tgcgagccgg
    29941 cattaaaggc gatttccgcg ttctgcgcca gcgatgcggc cacatagact tccatgccgt
    30001 agagattgcc aaacggcggc atggcgccca cttcgcaatc gggaaaccgc ttcttgaagt
    30061 cctcctccgg gacgagcttg gcctggtcgg cgccggtcat ctcgcgcaga tcggcaagga
    30121 caaccttgcg gttggccggc aggacagcca tagccatttc gccatcgaga tacacaatta
    30181 ccgtcttggc gagttcccgg ccggggatat gtgccgaggc ggccacctcc ggggccgtgt
    30241 aagcagtcga gtgaatgatg ctgacgtatt tgattttctc tttgtccaag agctgtttta
    30301 gtgtcgttac tggcataata ttactccttc cagcttgttg cctcacccta agccatctgg
    30361 cgggataagc aagtcccgga ggttccgaaa agcgacaggc cgtccagcga gaccaggcgg
    30421 gtcaactcac cggctgcctc aggatggacc tcgctatgcc cacaaaccgg ccaatcagcc
    30481 cccggccgaa gagctttgcg cgggccggcg aaaacgcgcc tatcttttca gactgaagca
    30541 ggttcaaaag gcttcgactg cggctcgcgt gaaggcgtca aagtgcggga ttggcacgcg
    30601 ccgccgcacg ctgacggtct tgccaggcgc gccaggccac ccaggcgcac accgccgcga
    30661 tgccaatcca acctgccagc ctcaccacag ccgctatgtg cgtgtcgtat tgaagcacct
    30721 gccagcggct gaagatggta ttccaatcat gctcgccgcc tcccactaaa ggcagttcca
    30781 gacgccgcgc atcggccata tagcgggcaa tattaagcca attctcgaag aaccaaatcg
    30841 ccgcgccggc cactgccagc ggatggcccc ggcgccagaa agacacagcc agtgcgcagg
    30901 ggaaaaccaa ttgccccagc gtgccgccat agggttcaag ccgcgagctg aacagaccca
    30961 ccagcggatg gcccgcttcg tgaaacagca agttggcatg gtccagcaga aaaacaaatc
    31021 ccggctcact gcgtaggacg agcagaagga ataaaccgaa gccaaagcca aagccggcca
    31081 gcttggcgcc ggttatcggt tcccattcac gcttttccat gaacctgaat gtcagcaaaa
    31141 cgctcgccag acgttttgct tatagccgcg cccggcaggg gatagcccgt gtttgtccaa
    31201 ggccaacggc ccaagcctga ccatgaccgc ctcgggattg gaaggctccc gggggccggg
    31261 cgtcttcgag cagactcaat tggcgcgcgc gaaggctggc cagccgcgca cggttattcg
    31321 gtgctgcggg aatcgccggt cgagtattcc gccagggcgc gcatgtattg ggcgcgctcg
    31381 aaggagctcg ggttgggaca gttcttctgg ctcaggctgc ccttgagttg agcgaccgac
    31441 tcatactcat gctcttccat ccaggcgatc agatcccgct cgatgacgcc aatctgccga
    31501 atgccgtgcc ggatgagcgc tgaacaaagc atggttacat ccgcgccggc catgagcatt
    31561 ttaagcacgt cggtggcgcg atggattcca ctggtcgccg cgaggctggc ctgcagtttg
    31621 ccgtacagca gggcaaccca gcgcagcggc acgcgcatcg ccatgggggt gcttagcagg
    31681 acgttggggc gcacttccaa agcctccaga tcaatgtccg gctggtagaa acggttaaag
    31741 agcaccaggc cgttggcgcc ggcctggtcg aggcgcttgg ccatgtaggc gaagttggtg
    31801 aaaaagggac tgagtttcac cgcgactgga atgctcacgg ccgctttgac cgccttgaga
    31861 atatccaggt agttctgctc aacgtcgcag gaggcggcgt tcatgtcggt gggaatataa
    31921 tagatgttca actccagggc gtccgcgccg gcctgctgca tgcccttggc gtattcggtc
    31981 cacccgccgg ttgaagagcc gttaaggctg gcgatgatcg ggatgcgaac cgcctccttg
    32041 gctttggcga tgtgttgcag gtattcctcc ggcccgagcc ggaactcgtt gggcgccggg
    32101 aaatacgtga gcgcctcagc aaagctctcg gtgccgcgtt caaggtgatc gtccaactcg
    32161 gcctgctctc gacggagttg ctcctcgaat accgagtaga gcaccacggc ggacgcgccg
    32221 gcgtcttcca tctgcttgat gccgtctatt tcctccgaca gcggcgaggc ggctggaacc
    32281 aggggagtgc gaagtttcag tccgagatag gtggtcttta gatccataag cctgaattga
    32341 attgttatgc gttaaggttg ttgagtttgc gcggaggcgg aactggcggg cgcattggcg
    32401 ccattgcccg aagccggttt aaggccggcc aggtattggt agtacgccca gcgccgatca
    32461 gcatcggcct gcgcttgggc gaagaatcgc cgggcgtctt ccggtttgct cttggtgagc
    32521 atcttaaacc ggttctccga gagcaggtag tcctgcactt tgattttggc cggactcgaa
    32581 tcgaagagaa gcgggctctc gccccgatcg aaacgccgcg ggtcaaagcg gtagagcagc
    32641 cattggccgg aatccaccgc catccgttgc tgccgggcgc ccacgccgcg gtccaggtca
    32701 atgccatgcg caatgcaatg gctgtaggcg atgatcagcg aggggccggg ataggattcg
    32761 gcttcgatga acgcccggag cgtatgctcg tctttggcgc ccatggccac gctggccaca
    32821 tagaccgacc cgtagcccat ggcgataagg ccgaggtctt tcttgcgcag cggcttgccg
    32881 ccggcggcaa atttggcaac cgcgccgcgc ggcgtagctt tcgagcactg accaccggtg
    32941 ttggaataaa cctcggtgtc cagcaccagc acattaacat cgtggccgct cgccaggacg
    33001 tggtccaaac ccccgtagcc gatatcgtac gcccagccgt cgccgccgat gatccaaaca
    33061 ctgcggcgga cgagattgtc ggccacggca agcagcattt tggcgtcact gctggagtcg
    33121 ccggccaatt tttgtttaag ggcggcgacg cgctcccgct ggtcgtaaat gcccgcctcg
    33181 tcagtctgct gggcctcaat cagcccggta acgaggtcgt cgcccagttt cggcgccagt
    33241 ttgcgcagca gctcgcaggc aaactcccgc tgcttgtcaa tcgaaacccg gaagccaagc
    33301 ccgaactcgg cattatcctc gaacagggaa ttgttccagg ttggaccgcg gccttccttg
    33361 tttttcgcgt agggagtggt gggcaggttg ccgccgtaga tggacgagca tcccgtggcg
    33421 ttgctggcca caatgcgatc cccgaaaagc tgcgtaagca acttcaggta cggcgtctcg
    33481 ccgcagccgc cgcaagcgcc gctatactca aagagcggct gcatgacctg ttgctggcgg
    33541 agctgggtga ccttgatctt ccggcggtca agctccggca gttcgaggaa gaatttgtag
    33601 ttgtcgcgct cgctgaaacg gatgggcggc tgcgggcgca tattgatcgc cttgagccgg
    33661 gcctcggtct tgttcttggc cgggcagacc tcgacgcaca ggcagcagcc ggtgcaatct
    33721 tccggggcga cctgcagggt ccatttcatg cctttccatt ccggagcccg ggcgtctgtg
    33781 ctcttaaagg tcggcggcgc gccttgcaga tgcttggcgt cgtaaacctt catgcggatg
    33841 ctggcgtgcg ggcagattac cacgcacttg ccgcattgga tgcaggtttt ctcatcccaa
    33901 accgggattt ccagagccag attccgtttc tcatagcggg aagtgccggt ggggaaggtg
    33961 ccatccgggc tgaaggcact gaccgggagg gaatcgccgc ggttgccata aataacgcct
    34021 aacgtgcggg tgatctccgc cggggcatcg tccgtgaacg ggggcggaag ctcccgggtg
    34081 ctggtaacct gcgcgggaac cttgacctcg tggaggttgg ccagggtctg gtccaccgcc
    34141 gcgatattct tcttcacgac ctcctcgccc ttgcggccat aggttttctt gatcgtgagt
    34201 ttgatctgct cgatggcctc gtcgcgcgga agcacgccgg agagcgcaaa gaagcacgtt
    34261 tgcatgatcg tattgatgcg ggcccccatg ccggtggatt tggcgacgct gatcgcatca
    34321 attacgtaga acttcgcttt cttcttgatc agcccctctt gaacgagccg cgggaggtgc
    34381 tcccagactt cgtcggcgcg gaaatgggtg ttgaggagga aagtgcctcc gggcacaagg
    34441 tcctggagca tgtcgtaccg gtctaaaaag atcggttggt ggcaggcgac gaagttcgcc
    34501 ttggagacaa ggtagatcga gcgaatcggt ttcggcccga aacggaggtg ggagatcgtc
    34561 accgcgcccg ctttcttcga gtcatacaca aagtagccct gcgcgtagtt attggtgttc
    34621 tcgccaatga tcttgatcga gttcttattc gcgccaaccg tgccatccga gcccaggcca
    34681 taaaagagcg cgcgaaccac tttgtccgac tcggtggaaa actccgggtc aaacttcagg
    34741 ctggtatgcg ccacatcatc ctcgatgccc acagtgaagt ggttcctggg agtggcggcc
    34801 tgcaagttgt caaaaaccgc cttgaccatc gccggggtga aatccttcga ggacagcccg
    34861 tagcggccgc ccaccacctt cggcatggac ttgatccgcc cccagcccgc ggggatgcct
    34921 tccgtcaggg cggcgatgac atccaggtac agcggctcgc ccagggcgcc ggattccttg
    34981 gtccggtcca gcaccgcgac ggccttgacg ctcgccggca gagcctcgac gaatcgcttg
    35041 ccgcagaatg gccggtagag gcgcacttta atgacgccga ccttggctcc gttttcattg
    35101 agatattcca ccgtctcatg cgccgtctcg cagcccgagc ccatcagcac caccacccgc
    35161 tcggcgttcg gatcgcccac gtattggaag ggctggtatg ggcgcccggt gatggcggca
    35221 aacttgtcca tcacccgggc ggtgatgtcc gcgcactggg cataaaacgg gttgcacgcc
    35281 tcgcgcgcct ggaagaacac atccgggttc tgcgcggtgc cgcgcaggac cgggcggtcg
    35341 gggttcatgg cgcgctgccg atgcgcaatc acaaactcct cgttgatcat ctggcggatt
    35401 atgtcttcgt ccaccagttc gatcttcgac acctcgtgcg acgtgcggaa gccgtcaaag
    35461 aagtgcagaa acgggacccg gctctccagg gttgccgcct gggcgatgag cgcgaaatcc
    35521 atcgcctcct gcacgctggc cgaactcagg agcgcaaacc cggtggtgcg ggcgtgcatc
    35581 acgtcgctat ggtcgccaaa aatggacaac gcatgagtgg ccagggtgcg cgcggaaatg
    35641 tggaagaccg tcggcgtcag ctcccccgcg attttgaaca tattgggcac tttgagcaac
    35701 aggccctgcg acgccgtgaa ggtcgtggtc agcgcgccgg tctggagcgc cccgtgcacc
    35761 gcgcccgcgg cgccgccttc gctttgcatc tccaccaccg agggcacggt gccccagatg
    35821 tttttaaccc cttccgatgc ccactggtcg caccactcgc ccatgttcga ggagggagta
    35881 atgggataga tgcccatcac ctcattgcaa cggtaggcca cgtatgccac cgcctcattg
    35941 ccgtcaactg tcgtgtaagt tttattcatg ttgcttgcgc gctcaatctc taaagctgtt
    36001 attgccgccg cgggaagctc cggctttcca cgactcatct ccgccagaat ggccaatcct
    36061 tatccagcga agcagcactg tgcctcaaac cgccctccgg caacttcacc ggggttggca
    36121 aaccttcacc atattttgcg ccccggcccg ttttctgaac atattcccgc ttccccgact
    36181 tcccggcgcc gaaatatccc gcttatgccg gggcgaactt gcgcaggtat tttatcgcgg
    36241 cggccagttg gcggggccag ggcgcggtga tgctcagcgg ttcgcccgtg agcggatggg
    36301 gcagggcgag ggtttcgcag tgaaccgccg cccgggcaat cagcgggcgt tccacctggt
    36361 ttggtttaag ccgatagtcg ggcttcaatt gtgagagccg caattcccgg ccgccataga
    36421 gtggatctcc cagcaccggc agccgcgcca cccgcaggtg ggcgcgaatc tggtgcgggc
    36481 ggtccgtcaa cggctcgcac tgcaaaagag tgtagccgga aaatttctcc cgcacctcga
    36541 agagggtcac cgaccgtttg ccgcgccttg cattcgcgca catcaggccc gcgcgcgccg
    36601 ggtgcggcgc gagtttggtg tcaatctgaa accgtgcctc cggcggcgtt ccctgcacga
    36661 gcgccacgtg ccgccgaccg ggctggctgg cgccgaagtg gttcagcagg gcgatgagca
    36721 ccggcttgct ttttgccagc agcattaccc cgctggtctc cgcgtccaac cggtgggtgg
    36781 ccatcaggta agaccgatcg cctgcccgcg cccagggcgc gccgcgtttg atgccgtcgt
    36841 gcagcagccg caacaggctc gggcgctccg gttgggcgcg gtcgggcacg gcgggcacgc
    36901 cgctcggttt atcgagcgcc agcacctgcg catcttcata gagaatcggg atttcccaaa
    36961 actcctgcgt ggccggcgat gaaagcttaa tggtgttctt catgctgcat ggcgcccggg
    37021 cgggggttcc cgcaacgtcc ggcgcaaccg ttccaccgcc agccctcccc cgcggaaggc
    37081 gaaaatctgc ctcccgcgtt tcccgtcgcc gcgcgcggaa cctaggattt tttctcgtcc
    37141 gacgccggat tcatggccgg cggggcctgt ggccgggcca gcggcgtgtc gcgcagtccc
    37201 ttctcgactt cctggcggaa ccagtcgttg aaggcttcat tctggcggtt caagcggacg
    37261 gcgttgagga aggccggcgc attctccgcg atcgtcgcct cgtccagcgg cagtttggat
    37321 ttcacgtaaa caatcacccc gccccggggc gtcatctgga aattgctcac cttgccggga
    37381 ggggtggaga aggcgatctg cttgagctgg ttgagattaa ggcgctcctc gatttgcggc
    37441 agttggcgcg tgctcagcga aaacggcggc aaatccacca acggatgctg ggcgtgggcg
    37501 caaaggtccg ccaccgtctt gccctgcgcc agcccgttcg tcagcgcctg gtaaaaatcc
    37561 atccccgcct gccgcgccag cagcagcgcc tggctgtggc gatactcttc cacgacctgc
    37621 acgcgaatct gttccagcgg cggaatttcg ctcgggattt tcttgttcaa cgagatgata
    37681 tacaccgcgt tcgatcccag gatcggcccg gccagcgggt cgtccggagt gcggctgaac
    37741 gctttttgca cgaagtcgag cgccacgccc aatcccggcg gcggggtttc gcggtcaaac
    37801 gggatcgtcg ctctgacggg gaacccatgg gcctgggcca cgatgtccag gttttccgcc
    37861 cgcacgggct ccatatcgaa cagcggattg gcaaaataca ccgcgttgct gcgggcgatc
    37921 tggtactcca actgtttgag ccgggcctcg cgcagcttcg ttttggcctc ggccaccgat
    37981 tgcgcgccga cttcccgcag gaaattagtg ccgctcagcc ggtacgcctc gtcaatctcc
    38041 aggtccaggt tggtcatccg cgccagctcc tggctggccg ccgccgtgta attggtcagg
    38101 tcaaacttca cgtagttgac ttgcacgcgc tccggcaccc ggtaagtggc cagccggttg
    38161 gaatagaact gggataccgc ctccggcagc accgtcacct gcgcctggta atccgccgcg
    38221 gaaaagaaca ccgcggccgc ggacagttcc tcgtgctccc gcttgaacag gtcgcgcgcc
    38281 tcctgggggg tggttaactt cccggccatc ccggccgtgg caatcaactc ctgcacgccc
    38341 aggtaatgcc gtacaaagcg ctcgaagtcc tccagtccga acccgcgcgg ccggagcacg
    38401 cgctgcacaa acatgtccgg cgaggtaatg ccggcgcgct ggaactggcc gatcattgac
    38461 cgggccgcct gggctaccac gtcggtgctg atatgaatgc ccagccgctt ctgcttctgg
    38521 atgagcagca gccattgata ggtttcccgc tcgatttccc cgcccgcttt ccgggactca
    38581 tcatccggcc aatcgcccat cataaaataa tggcgcaagt aaacctcgcg ctgggcctgg
    38641 ataaactgct ccgggttgat gcgctcgcca ttgatggacc ccaggttgat ccgcccgcgg
    38701 ggggcatcgt tcagcttcga gtaaggggaa aagaagatga caaagctgat aatggtgagc
    38761 gttataataa tcgcccacag ccaggtctga tgcttgcgaa tggtcccaaa cataatttta
    38821 tacggttaaa gaaactccac cgtagcggcc tttgaacccg cgggtcaaat ctaaaatccg
    38881 gtgaatcctg cccaacgccg caccccgcgc tccggcccgg gccggcggaa ccgttccgga
    38941 gcgggcgttc ctctggaccc acgctccgca gcctcctgcc gggcggcctg gccgcgggcc
    39001 gccaccgcct caatcgtcgg cggcaaagaa cgtcagatac gtatcgccgc gagcttcgcg
    39061 caacagcagc aacgccagtt cccgggcgtg gtaatccccc cacatcgagc tttcgccgcg
    39121 cggcgtcctg gcgccgggag gagcataatc ccaaccattg gggcggtgat agatcgagtg
    39181 gagcagcagg ccctggtgcc gggggcgggt ggaaaggtaa ggctcgtcga agagcgtgtt
    39241 tgccaccgtc agccccgcct gccggtagcg gccgcccgcc ttggcgtcgc cccggcgggt
    39301 gaggtaattt ccgagccgga ccaggccctg gccggcaatg gcggcggccg agctgtccac
    39361 gggttcccag tcgttgaagg gatccgccgg ctggctcgcg taatcgccca gccggtgcaa
    39421 atgaggcgct cccgtgtccc acattgggat gccgtccgcg caggtgtgcg ccaggtagaa
    39481 atccgccgtg gcccgcgccg cttcgaggaa caaggcccgg ctggcctcta aatcacgccc
    39541 ggcgtctgcg gccgccggcg gccgctcgtt cgcccgtccg gcgcgcctgc cgtgccccgc
    39601 gtcgagaaac tccagttgct ccgcgtagcc gagaatggcc caggccagcc cgcgcgtcca
    39661 ggtgctgaac ggcgaatagc cctgctgcgt gttggggcag cgataactgc cgtcgttgag
    39721 attgaagatg ctctcgtggg ccgtccggcc gcgcacgtcg aacgtgtccc ggcccttgcc
    39781 gtagtaaatg ctgtaaagcg ccgtcgtttc ggcgtggtcc aacagccgct gaaacagcga
    39841 gatgctgcga tcccgttccc cgcgcagcac gtggccaagc tggtgcgcca ccgccagcga
    39901 gcgcagcgac cgcacggtgt ccacaaacag ggagtgcggc ccgttgaagg agtagatgta
    39961 gcccgccccg tcggcggtcc gggaccaccg cgccgcctgc accgcgccgg aaaccttcag
    40021 cgccagctcg tacagctcgc gctcgggctc gttgccggga atcttgccct cgcgcatcag
    40081 ccgccacagg ttgccgtagg tcgagatatt gttaaacccg tgatcatgca cccccaagtt
    40141 cgagacatgc ggcgccattt gccggatcgt ctgccggcgt cccagttcga gaaagcgctt
    40201 ttctcccgtg gcgtcgaact ggagcagggc cgcgccgaac tgaaatccct gcgtccagtc
    40261 cgtccacgcg cgcgacgtgt attggccccc cgcggtgacc accggggtgc cgcgcgccgg
    40321 gtcccaggtc cgttcgaggc tcaaaatctt ctgtgcggac agcgcaaaca accgttcgac
    40381 cttgggccgc aatttttgcg cggtcagatg atcatcaatt tgcatgggca ttagagtctc
    40441 cttaaatgaa agccgccgtc cacattgatg acttccccgg tgctgaaggg cagcaggtcc
    40501 tgcgcgatgg ccgcgacggc tcggccaacg tcttccggcg tgccccaacg cgggatcggc
    40561 gtcagcccgg cttcaatcag ttggtcatac ttttccttta ccgggccggt catgtccgtg
    40621 ccgataatcc ccggacgcag ttcataaacc aggatgccat attcggccag ccgcgcggcg
    40681 tacagcgcgg tgagcatgga caacccggcc ttcgagaggc agtaatcgcc gcgattgact
    40741 gacgcggtgt aggcgctgac cgaagagatg gtgacgatcc ggggccggta gttggccggc
    40801 ggaatgtccg cctgcccctg ggcgatcatc caccgtgccg ccagttgcgt caggaaatac
    40861 ggccccttca aattgatggt aatgagccga tcaaagctct cctcgctcgc ctcgagaatg
    40921 tcagcccgca cttccggcgc caccccggca ttattgacca gcaagtccag ccgccccagg
    40981 gttgtcttcg caaactcgac cagcttccgc cggtccgcgt cttttgcaat gtccgcctgg
    41041 caaacttcca cccgtacatt ccggccggct gcctgcgcgg ctgccaggca atccgccgct
    41101 gtctggcgcg ccgccactgc gttgccggcg taattaacaa tcaggtcgta gccgatcttt
    41161 gccaattcca gcgcaatacc gcgaccgatg ccgcgcgacg caccggtaat catggcaacc
    41221 gcattcatag gtcccgagga tagccccttg cctccgaatc aaaagaaaat agtgaaagca
    41281 aagccgggtc cctccgaaag ctcgaaaata aacccagcgc cagccaaacc gatcatcaat
    41341 ccagtggagc cgcgaagtgt ccaattattg gacacttccc gaaaagccat atctggcggc
    41401 taaatcagcc cgccagacct tatttctctc gtttgtacac aatccccatc gtctcagcgt
    41461 cttacagggc tttcagtaag ccgtgtacaa ggggtgtaca agggatgctc atcgggtgta
    41521 ccagctaaaa tcctatggac tcccggtgag catcccttgt acaccccttg tacacggcgg
    41581 aaaagcaggg ggaaatgagg gatattgcag ggaattgggg ggccgcgagg gctcagatca
    41641 tggttcgggc cttgatctgg ttggctattg cttgaaccag cgcttcgtca ggttctccgc
    41701 cagggggcgg attggcgggg cgggcggcac aatgggccgc cgcgcaggtt tcgccgggac
    41761 gccattcggt gctcaggcag tgttcgcatt gttcgaggcc gtagcgcggg tcgggtatgc
    41821 cctggcgctg cttgatctgg agcaattcct ggagttgggc cggggttatc cgcttgagcg
    41881 gtttccccag ttgcgaggcg atcagcaccg tatggcagta ggcttcgagg gtttcgatct
    41941 tgaaataagc gtcctcaaca ctccgatggc tccaggcgac cacgccatga ttggccataa
    42001 ggatggtgtt atgctggtcc acgaggtcgg cgacgaaccg gcccagttcg ggagtgccgg
    42061 gggtgcgata gggcgccgtc gccagcgaga tggtgagctc gaactccgag gtcaggcagg
    42121 tgggcggctg gactccggcg atggcgaacc cggtgctgta cggcggatga caatgcaccg
    42181 aggcaaccgc gcgcggctgg cgctgcatga tctggagatg catcaggatt tcgctggtgc
    42241 gttttcgggc gcccgcgagt tggtttcccg ccagatccac caggcacaag tcctccgggc
    42301 gcatgaatcc tttggagatc tgcgtgggcg tgcatagcgc caggtccggc cccacgcgga
    42361 gggcgagatt gccgccgttg ccgtccacgt aggcgcgctg ccacatgcgg cggccaattt
    42421 cgcagatttg ttgcgccagc tcacggacgg ccggcgaggc caggaaggct tccaggtcgg
    42481 gccggggact ccgggcagtg attccgtcga aaggcgagcc gcccgccgcc aaccggcctg
    42541 gccacagccc ggaagctccg ggatcgcccg gcgtcgcgga tgcggaaggc gtctttgcgg
    42601 ccatagctgc ggatactatg acagatttgg agcgttcgcc aatcccggat tgcgagggca
    42661 aggctttgcg aggatgacca ggcccggggg gcgcccgccg tcaaacgcgt ctaaaacagc
    42721 cgggcgccga tcagagccgg tgcagttcct cgatgagggg ccggctgtag gcatcataga
    42781 gcagtttcag ggtgagcagc aggacgaccg agacgaaaat ggggcggatg attttcacgc
    42841 cccgcttgat gaccatgcgc gcgccgatcc gcgcgccgat tatctggccg gcgcccatcg
    42901 tcagcccggc ggcgtaattg acatagccgc ccggcaggaa gagcaggagc gagctgatgt
    42961 tgctggcaaa gttcatgact ttggcgtggc cggtggcccg ccgcatgttg aagcccagcc
    43021 cgagcatgaa ggccatcgtc cagaacgtgc cggtgcccgg gccgaagaag ccgtcataga
    43081 agcccatcaa cagcccgaag aagaaatcga agcgttcgcg ccggaagcgc gccgggcggt
    43141 cgccgagtcc gaggttgggc ttgaacagga catacagggc gacgctgaaa agcaggatgg
    43201 ggatgccgcg ccggagaaag gaggggtcaa tctggcgcac caccaacgtg ccgcaggctc
    43261 cgcccacgaa cgtgaacaga aatccgcggg tgcattcctt gaaggccacg gttcccgccc
    43321 gctcatagta ccaggacgcg cccgcggaac cgaaagccgc ctgcagcttg ctcgtgccca
    43381 gggcatggcg cgggctcaga ccgcaactga gcagcgccgg cagcgtaatc agcccgccgc
    43441 ccccggcgat cgaatccaca aagcccgcca ccagggcggt cacgaatagc accggcaggt
    43501 gccaaagttg cgtcatagat gggagtggct ggacagcgcc caaagcagga tggcggcggc
    43561 ggtgccgtcc atgatcggct cattggaaga gtaatcctgc acgtcgtcgt gatagaccgc
    43621 cgggccctgg aaggcggcca gcgggtcggg ttcggtgatg gccacgcctt tcagcgatcg
    43681 gaagatgcgt tcgtacaccg gcccgtccac cagcccgccc cggatcgggc gcccggttaa
    43741 ttgcatggtc atcaggtgcg ggtctttggg atagacgctg ccgatcccgg tgaacagtgt
    43801 gtagccccag ggattgcgtc ccagcagcca gtcgcgttgc tgggccgcga agcggcgata
    43861 cgtctggtcc ccggtcatcc gctcatacag caggcattgg gtggccaggg cgacggtgag
    43921 gttggccgag caccagatga acggcacgcc gatgcggtag gggttttgcc ggcccatgcg
    43981 ctcgcagcgc tcgatgccct cgcggtagta ccccgcgagc attttccgga aggggcgatc
    44041 caccagcccg taaagccgga agtggccggc gttcaggaag gggaaatact ggtaatggcc
    44101 ggtctgctcc tggcccatcc aggattccgc ggcggccagc cgggcgtagc gccgggcgtc
    44161 ttcgagatag cgcggttcgc gcgtggcgcg gaacagttcc gccgcgcccc attccatgtc
    44221 gtcggcccag gtggtttctt catagcgata gggggcgctg taggaattgc cctgctggac
    44281 gccttcgcgg gcgcggccca gctcatacac ttctttgccc gcctgcaggc agcgttcggc
    44341 gaagggccgg tggcgcggat cgtttcgcca gatatggtaa gccaggctca tggctgccgc
    44401 gtagcggccg gccaggttgg ccacgcccgt ggaggcgctc tggtattggc gcagtccctg
    44461 cggctggccg tcggcgaaat agaccacgcg cgggccgccc ttgccccagc catagtcggc
    44521 aatctcgttt tgcggcaggc ggcggccggc gtgatcgcgg tcatcggcca cttggtggta
    44581 gagctggtcg ggcgccggat gcagtttgag catccactcc agcccccagc gggcttcgtc
    44641 gagcacgtcg ggcaggccgt tgggataggg ctggcccagc gcgttgacct tgtccgcgaa
    44701 gagctggcgg tcttcggcca gctcgtacgc gagcagcatt tgcgcggtgg cgttgccgga
    44761 ggtgagcagg tatttcagca aatccgccgc gtcatgccag ccgccccggg cgtcgaggta
    44821 ggtgccgttg gtgagggggc cgtacgccgt gcggccgtca aacgggtggc acaccgcgtc
    44881 cagccagggg ttgtagccgc agcgttgctg gcgcataaac tcgagcagtt gatcgggcag
    44941 ctcgtggtag gccgccgcgc cgagggtaaa cggcagtgat tgcgcttcgc ccacgcgcag
    45001 cacataacgg cccggcgtcc gcaaggcggt gaagtccagc tccgcatggt gctcaaactg
    45061 gccccatcgc acgccggtga tgggccgggc cgaaccggtg aaccgggctt ttccggtgtc
    45121 cgcttccgcc actgtgaagg cggccgggag cggcgctttg gcaaaggcca ccccgagttt
    45181 cgtgtcgcgc gtccggtagc cgatctggct ggcgcgcagg aagatttccg cggccggggc
    45241 gtttgccagg ccagccgcga ggagcagcag gagaacacag agatgaggca tacgttttgg
    45301 gggttggcat cggttgccgc gccggccgcg cctgggcggc gggccggcgg cggcggtggc
    45361 aaggcggagg gattatccgt tagtagagtt tctgcaattc cgcgccgcgg aagtagtgct
    45421 tcacgatctg ctcggccggg atgccccggg aggccatgac ggccgcgcca atctggcaaa
    45481 agcccacccc gtgcccccac ccggcgccgc gcagggtgaa ccgcgccggc gccgcgccgg
    45541 ccgcgccctc gggttgcacc acaaacgcgc tgctgtacag gtgcgagggg ctcagccagc
    45601 ggcggatttc caattccttg cccaccacca ccgtccgctg ggagccgaca atgcgcagcc
    45661 gcacgatgcg gcccgacggc ccgcgctgca gcggttccag ccgctggatc acgccgaagt
    45721 ccaggcccga ccgcttgcgc aggatcgcgc tgagttcccc ctgcgtatag ctgaccgtcc
    45781 agcggtagaa gtccgtggtc tcctggtcga acgtcggcag cacctgccgg agaatgtctc
    45841 cgtcggtcgt attgcagtag gcttccgggc tggagcatat ccactgctcc gcggcggctt
    45901 ccgaggtgat cggcgggtgt tccaccgccg catcggatac gcttgccagg tacggcacat
    45961 ctatcccctc ccacgcggag gaaaacagct cgctgcgccc gccgcatgac ttggcgtagc
    46021 gcgcatcgca aatctcgccg ccgtgaacca ggaacacgcc ccgggtatcc cgcaccgcct
    46081 cgatggcctt gggcgaaatg attttggtga gcccgtggta gcgctggcag tggtcatcgg
    46141 cgcacacatc aaagaaacgg tgatcttccc gggtgtacca gcggacaatt tcttccgcgg
    46201 tttcctgccc ctggcgcgcc gggccgctgg tcttgccggt cagcttctgc cgttcgagca
    46261 tggcgacgag ccagctccgc gaggtgatgg cgtgtgcgcg caacagctcc agcggcgccg
    46321 tggcgctcat ttccgacgcg atgacgcttt tgaggtattg ctcgaccccg gcctcgttga
    46381 tcgccgtcac cgtgccgtcg gcgttgagca ggagccgcag cgcgccttca aacgtttcgt
    46441 cctcctttcg ttcccagtga aagttgatcc cgatcgtgac atcccgcagg gtgaacgtgg
    46501 cgtcgccttc cggggtgagc cggatctccg gcccgcgcgc gagttcgcgg ccgtcaccgg
    46561 cccgcagcac cagttggccc tcgccggggc gggcggagaa ttctccgctc accgccgtct
    46621 tgtccgggag gaggaagcgg ccgttcaggc agccgcgcgc ttccggctcg cggctgtaga
    46681 tgccaacagt aatcagaggt tcccacgtaa tcataaactc gggtgccggt tcaccccggc
    46741 ggcaaaatct tccacaaact tgacgcactg ggcgacgtcc tggaccagtt gcggcgagag
    46801 gtgctcatat tcgatggaca tcaccccgcg ccatttccag tcatacatct gccgcaggag
    46861 ggcggcatat tgggcctgcc cggtgccgag gggcacgtcc cggctgtcgc gccggccgga
    46921 ttccgcggcg tccttcaggt gcaggacgag gattcgccgt tcgtatttcc gcaggctcgc
    46981 cagcgtgtcc agccccgagc gcacccagtg gccggtgtcg gggcaggcgc cgatgcgcgc
    47041 gctgtggccg gcgcaagctt tgatcacggc gtcggggttc cagtatttcg agttggggcc
    47101 ttccgggtgg ttatgcaccg ccaggttgat cttgtattcc tggcagagct tgtccagcgc
    47161 gccaaagttt tccgccggcg gttcggcgat cagcgtctcg acgcccattt ccttcgcgaa
    47221 atcatagacc ttgcggtact cgctggtatc cttttcgagc ggggcgtagt aggtgggcat
    47281 ggtgaagccc aggtcccgca tgcgctcctt catttcgcgc cgctgcgccg gggacaggtt
    47341 ctcgctggtt ttcaggtccg gccgttcctt gctcaagggc aggaagaacg ccggttccac
    47401 ccggcgcaag cccatctccg ccaggacttc cagcgactcg taaaagctgc ggtcgcggaa
    47461 ggtgtaaagc tggcaactca gccgccagcc cagcttctcc gcccacggcg tgcccggttt
    47521 ggtccggcgc cgccgccccg gcgccgccgc ccgggccagc gagcccggcg ccagcatggc
    47581 cccaaccccg gaaataaccg tacgccccag aaatacacgg cgattgcaga tgttcattgt
    47641 catcgtgatt cgtctgttcg tttgttaaca gccttgcggc caaccgtttg ccgaaggtta
    47701 aatacacgac ttggcgcccg ggtgcaagga atcttattgc gcggccgccc gcgcgcgatg
    47761 gcagccgggc gggcgcaagt catggccggg acggctccgg ctggcggtcg cgcagggggc
    47821 ggccggcctg gaagaggcgc aactgcgcgg ccagggttcc cgccagggcc tggttctccg
    47881 acgccagggc cagcttgagc gccttgtggg cggcttggac ggcggcggga aaatcgcccg
    47941 cctcggcgct ggccacagcc agggtttcga ggagccgcgc atcctgttcg ccggtcagtt
    48001 ccacggcgcg ccgggccagg cgcacggctt ccgcgccgtc ccggctgtcc gcgttctcat
    48061 gtgtcgccag cagccaggcc agcccatcga gcgccgtcac catgtccggc tgcagttcaa
    48121 gggcccggcg caagagcggg atggcttcct ccggctggtt cagcaggact aaagcgtgtc
    48181 ccgcgccata cgccgccgct gcgtggtccg gtttccaggc cagtgccgcc tgctcgtgaa
    48241 acagcgccgt gcggagttga tccgtcagct cggggcctgg ggaagcgggg ttgggggcgc
    48301 tggcgttggc cgacagttcc ggccggcatt tttccaggat caggcaggcc aggttatggt
    48361 gcgccagcca gcagcgggga ttgcgtttga gcgtggcgcg ccaaagcgtt tcttcgtcct
    48421 ggtaaatccc gcattgcgcc cacgtcaacg gccccagcac cgcgattgcg ctcgcggccc
    48481 cggccacgcc cagagccttt gccagccgcc cgcgcgccgg gcgcgccgtc gcggccagtt
    48541 tttgcgccag ccacgcgccg acgcccgtca ccagtgcgat catcccaatc atggccgggt
    48601 aaacccagtg atcggatacg aacgaatact tctggaaata aatcttgaag aagcccagca
    48661 cggggaaaag catggccaga aaataggtca gggcgaaaaa cggcgcccgc ccccagcggc
    48721 ggcgatgcca ccagctcaac ccccagattc ccaacagcaa aacgccgggc agataattca
    48781 cgacctcggc gggagaaaaa ttccagcgcg gatacacaaa catcgggttg cggggaaaca
    48841 cgatccgcgc caggtaaaac cacccggcca tgcccgcccc caccagccgc gaccggaaat
    48901 cgcccgccgc gaccacatcc gatccgatgc tgcgctcgta ttgaaaccag attgtcacca
    48961 gcgccagcac cagcccggcg gcaaagaagg gcagcgtgcg cccgacatcc cccttcgtca
    49021 gcggctggcc gtccgcgctg cttcgccacc aggcgcatag cagcagcacg acgggcagca
    49081 gcacgacgga ggttttactg agcaacgcca gcacaaaggc gatgaacgag gtccagtacc
    49141 agaacgtttc cgcgttgaat ggcgccgccc ggttcggcgg gctttttccc ttcggcctcc
    49201 gggttttccg cgctcgcatt tccgcatccc gccgcgcctg ttcagaacgc aaataggcca
    49261 ccagcgccag gccgaagaag aacagtgaca acgtgttctt gcgctccgtg atccacgcga
    49321 ccgactggac gttgatcggg tggatggcaa aaaggagcgc ggccagccag gcccccggga
    49381 tgttcaggtg gcgtagcgcc cgccagagca gaatggccgc cccggcgtgg agcaggatgt
    49441 tggtgacatg ataccccgtg gcttggcctc cccagagccg ccattccagc cagaacgatg
    49501 tcagcgtcag tggccaatag tcgtagaact tccctccgct ccagatatgc cacagcccat
    49561 ccggcgcgtg gattaacgga ttggccgtca ggatgtcgtc atcatcccag ataaaacccg
    49621 cctgcaccgc cggccaatac accgccagcg tcgcgagtaa aatagcggcc gcgccaatca
    49681 ataagctggt ttgcttcgtg gtcttgatct tgcgccccag cttaaacctt tcccacgcgg
    49741 aagataagaa taaaagcggc cgccgccaaa aagccgggag ccgcccctgc tttccggcag
    49801 ggaagtgcag accgaaggcg aggctgcgct caagcccatc ctgagcgcag gtgccgttct
    49861 cgttattttc ggccataaat ggagaagagt cggaagctga cagagtttga atttgggagt
    49921 ggcgcggccg cgaataaaag tgtaggcttg gcgtcaaagt gaaggtaagg ccatgaacag
    49981 gcagatccta attgatcgag ataggcgcgg acatgctggc gcagccgcgc aacggccttg
    50041 ccgccggatt ccaacgcccc gggcgcggcg ggtgcactga ctttttagga actgaattaa
    50101 actcattagc ttatgaaaat agtaataact cgaatgacag catcggtttg cggcgtgatg
    50161 atggcggtgt tggccgccgg ttgcgcctcc agcggcgtga agaatccttc cggcgtgccg
    50221 gtgacgcaca tgaacgccga cgaacagggg tttgtggcgg gcaccggggt ggaatcgcag
    50281 gatttggtga tggtggccga caagatgtcc cggagcatcc tgggcatccc ccagatcgcc
    50341 aatgcggcca cgccgccgtc catcgtgatc aatcccgtgg ataaccagac ccgctttccc
    50401 attaacaagg acattttcct gacacgcatc cgggcgcaac tgaactcgaa ggcgcagggg
    50461 cgggtggtct tcctggcgcg ccaccagatg gaagagctgg aaaaggagcg caaccgcaag
    50521 cgcgaagggc aagtcacttc cagttctgat cccagcgtgc aggagttcca gggtgcggat
    50581 tacttcctga ccggttcgct gcaggggctt tccacccgca ccaaggccgg cacgagcgac
    50641 tacattcttt acaacttcca cctgattgac gcgcggacca gtgccatcgt gtgggaggac
    50701 atggccgaat tgaagaagca ggggctggaa gacgctgcct accgctaggc agcgagaggg
    50761 ggcgcagtga tgaaactcct ctggcagccg gtgctgctgg ccctggcggc aagcctggcc
    50821 ggctgcgcga cgagccggaa gccggcagtg ccgccgctga cgggcgacat tctggtggat
    50881 gcgccccgag ccattgccag cggcccggcg cgcgaccgct tgatctggca ataccggctg
    50941 gcggcggcgg cgatgcggca ggggaagttc gacctcgcca agcagaacct ggatgaagtc
    51001 ctgctgacgc tgggcggcat ttacggcaag gacccggatg caaagaaagc gcgcagctac
    51061 tttcgggcgg aggcccggaa aaccttcatc ggcgagccgt acgagcggtc tatggcctat
    51121 atctatcgcg gcatacttta ctggatggac ggcgaacgcg acaacgcccg cgcctgcttc
    51181 cgcagcgcgg agttcgagga cagcgacgcc gcgctgcagg aatatgccgg cgactgggtg
    51241 ctgccggact acctggatgg cctggccacg gccaagctgg gcggggacgg ctcggatgcg
    51301 ttccagcggg cgcaggctaa cgccaaggga atcaaactgc cgcccgcccg gcctgaggcc
    51361 aatgtgctgt tttttgtcga gttcggcccg ggcccgacca agtacgcaac cggccaatac
    51421 cagcaggagc ttcgcttcaa cacgccggct tcgcccgtgg cgtccgcgat gctgaaggtg
    51481 gacgcccggc aaatcccgat tgctcccacg gacgatgtcg ggttccaggc caccacgcgc
    51541 ggggggcgcg tgatggacca catcctcggc aacaaagccg tgttcaagtc cgccacggac
    51601 gccgccggat cggtggcgat gctgggcggc atcaccaccg cggtggccag ccgcgaccgg
    51661 acgacgcagc aagtcggcct ggggattgcg ctggccgggc tggcgacaac gctggtttcg
    51721 gcggcgaccg tgccgaaggc ggatacgcgg gcctgggata acctgccgcg ctacctgagc
    51781 ttcgcctgcc tgccgctgcc cgtggggcaa catgtcgcca ccctccaatt cctcgattcc
    51841 gccggccagg ttcaatcgaa cctgaccaaa accatcaccc tcaacatcgc gacgacggac
    51901 cgggacaagg tcgtcttcgt aagcgatgct tccattacgc cgcaaacttt atgaaaagag
    51961 cattcccatt actcctcgcc gcgggcagcc tcgtgctgct ggccgccggc tgccgcacct
    52021 acgataaagg gccgtatctg ccgcaaacct ccaaaacgcc cgcttacgag agcaaggaac
    52081 cggtgatcct gatggatccc gcggtccggc gctcggtgac gtgcagcggc ttgcaggagc
    52141 gaacgctgcc ggatggccgg ctggagattg tcgcccacct gcgcaaccgc gagaaccgcc
    52201 gcattgaggt ccaggccaac tgcgtcttca aggatcagaa cgccttttcc accggcgacg
    52261 aaacgccctt cgtcatggtg attctgaccg agaacggcac ggaggatgtc aagttcacct
    52321 ccatgaacaa tctcgcgaag aaatacacca tccgcgtgcg ccaggcacgg taagcggccc
    52381 gattggaatc cgccggaatc actgcgttca ccggcgcggt tcagcccatc cccgggcccg
    52441 gcggccctcc gtttcaacgg ccccggcagc ccggcgaaaa aggggagggc ggcaaaaagt
    52501 gaggcgcgcc cgcgcccgga tgctggccgc ctcggcgtgc tttggcaacg caacgaagcg
    52561 ccatgcccgc tgaaaaagag atgaatatgg gtattgccat ttcgtcagag ttgtaataag
    52621 cctgatggct gctagagctt gactattgac ttattctatg aactcgacgc cgaaccaaac
    52681 tgcgaatccc ggagaatccc gccgcacctt catcaagaaa gctgccactg cggcggcggt
    52741 ggtgagcacc gccaacttgt tcaagacccc ggtctatggc cagagccagg cccccgcccc
    52801 ggggcgcgtt ctgggagcca atgaccgcat taacgtggcc tatatcggca cgggcaagca
    52861 ggggatgatg cacatcacca ttgagaaaaa atatgcccag gacaacaaca tcgcccaggt
    52921 ggcggtgtgc gacgtttatc aacggcacct ggacatggcc cggaacgcga tcggcgtgag
    52981 tgagagcagc gcctataaag accatcgcaa gctgctggag cgcaaggata ttgacgccat
    53041 cgtggcggcg ccaacggatc cgtggcacgc acaggtcagc attgacgcgt tggaagcagg
    53101 gaagcacgtg ttctgcgaga agcccatgac gcgctacctg gccgaggcgt tcgaggtttg
    53161 tgacgctgtc aagaaaacgg gcaaaacgta cgtcatcggg tcgcagggct gcatggacgc
    53221 caagtggcat aaggcggcgg agtgggtcaa ggccggcaaa ctggggccgt tagtctgggg
    53281 gcagggctcc tattgccgca acaacaagaa caacagcgag tggacgttcc ccattgatcc
    53341 cggcatgaat gagcagaaca ttgactggga acgctggttg gggaaaaccc cgaaaatccc
    53401 tttcaacccc gagcattatt ttagctggca caaatactac gcctacaatt cgggaattat
    53461 cggcaatctc ctgccgcacc ggtttcagcc gctgatgctg gcaaccggcg accccgaatt
    53521 cccgcgccgc gtcgccgcca ccggcacgcg caaggtttcc accgaccgcg agattaccga
    53581 cacgacgcac ctgctggcgg agttcccgag cgggctgacg ctggcggtcg tgggcacgac
    53641 ggtcaacgag cagggcctgc ccgaagtgtt gcgcgggcgc aaggccacct tgcacttcgc
    53701 gtcgagccag aaccgggtcg agttgaagcc ggaggccatc tttacggacg aactggaggc
    53761 cgaggaattt accgacccgc agccgacgga acgcatcgaa cggctggaaa aggacttctt
    53821 tgactgcatc cgcagcggca agcagccggt ggccaacgtc gagctggcca ttcgggccca
    53881 aaccgtgttg tgcctggcgg aaatgtccga gcggctggga ttgacgctgt tcttcgaccc
    53941 caagacccgc gccatcaaga ccggcgacgg gcgcgtggtc ccgccgctga gctatgacag
    54001 cgtggtgccg ccgatgggct aggctttctc ttccagcaac tcgcggcggg cgccgctgag
    54061 aagttgctcg ataaacgaag tggaataggc gccgcggcga aagttgggat cctgcaggat
    54121 ggcctgctgg aaggaaatcg tcgtcttgac cccggtgatc atgtactcgg ccagcgcccg
    54181 gctcatcttg ttcatggttt cgcgtcggtc gcggccatag gtgaccagct tggcgatcat
    54241 ggagtcgtag gcggacggaa tggtatagcc ggcgtaggcg tgggaatcca cgcggacccc
    54301 gcggccgccg ggctggtaat acatctcaat ccgccccggg ctggggcgga agttatcgaa
    54361 ggggtcttcg gcgttgatcc ggcattcgat ggcgtggccc ttgagctgaa tgtcactctg
    54421 ggagtggcgc aggggctccc ccatcgcgag catgatttga tagcgcacca ggtcaatgcc
    54481 ggtgacttcc tcggtcaccg ggtgttcgac ctggatgcgc ttgttgacct cgagaaagta
    54541 gaaccggcca tcgttatcca caataaactc gaccgtcccg gcgttggtat aattggccgc
    54601 ctccgcgatc ttgatggcgg cccggcccat gcggtcccgc aacttccgga acttggggtt
    54661 ctccagcagg ggcgacggcg attcctcgac gaccttttgg ttgcggcgtt gtatggagca
    54721 atcccgctcg cccaggtgaa taatgcggcc cttgtgatcc ccgagaatct ggaattcaat
    54781 atgatgggga ttttcgatgt atttctcgac gtaaaccccc gaattcccga acgccttttc
    54841 cgcctcgccg cgcgcggtat gatagccctt gatcagcgaa atgtcgttgt gggccacgcg
    54901 cattccccgg ccgccgcccc ccgccaccgc cttgatcatg accgggtagc cgatgcgctt
    54961 ggcaaccgac agcgcgtcct gttcgctttc gacgacccct tccgagccgg gcggggtggg
    55021 cacgtcggcc ttcttggcca gggcccggct gacggccttg tcctccaggg cattcatggc
    55081 ccgcgagttc gggccgatga accgaatgtt gcagctctca caaacgtcgg cgaaatgggc
    55141 gttttccgcc aggaacccat agccggggtg aatggcgtcc acgtcggtga tttcggcggc
    55201 actgacaatg cgatcaatcc gcagataact gtcatgggag ggcgccttgc caatgcagat
    55261 ggcttcgtca gccagttgca cgtgcatgga attggcatcc gcttccgaat aaatggcaac
    55321 ggtgcggatg ttcagttcct tgcaggcgcg gataatgcgg accgcaatct ccccgcggtt
    55381 ggcgactaga atcttctcaa acatgcctgg cgacgataca gcgttagggc ttagagacga
    55441 actccccggt ctgaaatcac gggaacccgg tcacgacggg cggattttga acagcacctg
    55501 cccaaattcc accggcttgg cgttttccac catgacctgc gtgatgacgc ccttggcctc
    55561 cgccttaatc tcgttcatga ccttcatggc ctcgatgatg cagaccaccg tttccgggcc
    55621 aacttccgtg ccgacttcga catacggggc ggattcgggg gagggcgcgc ggtagaaggt
    55681 gccgatcatg ggggatttga tttccaattc gttgctggcc gccggcggag gggcggccgc
    55741 gggaaccgga gccgggggtg gaaccgcaag gggaggggca tacgccacca gcggcgtatc
    55801 ttcatgcgcc gccgccgcgc cacccccatt ggctccccgc ttgagcctga tcttgaaatc
    55861 ctgcttctcg agttcgaact ccgtcacggc gttcttcctc atcaggtcaa taatagattt
    55921 gatgtctttt aggtccacag ccatctatga ttagagttac actatccgca cttaacccct
    55981 gcgttccgta tttcgattgc tattgaaggt atcaaacccc attgcgagtc gtcaacaatc
    56041 gaatccaact cttttttgcg cggcaaaacc tcctcgaatt cgcggcaaaa aaggccggtt
    56101 tagccgtgtt ctcagctctt gttaacgtta acggccgctt ctaacgctaa aagataggat
    56161 tcggcaccaa aacccagtat ctgccccagg cacaccgggg caatcagcga tttgtgccgg
    56221 aaatcttccc gggcatgaac gttggacaaa tggatttcaa tcgtggggac gcccgccgcc
    56281 gcaatcgcat cgcgcagggc gatgctggta tgggtgtagg cagccgcgtt caggacaatg
    56341 acatcccatt tccccttagc ttcctgaatc caactcacaa gctggccctc caggttgctt
    56401 tggcgaaagt ccaccactac cttgcgtttt ttcgcctggg cacgaaccct ggcctcaatt
    56461 tcagccaaag tcgcccgtcc atacacttga ggctcgcgct ggccgagcag gtttagattc
    56521 gggccgttaa gatagagtat cttcatcggg cggactgaaa ctagcgggaa taccggggct
    56581 tgtcgaatgg atttattccc gaatcggccg ccgccacggt cccttcacgc atgcggacac
    56641 gcatcgccgg aagcgcgctt tgcgaaggga aacccggccg aatatcgctt gccgggacgg
    56701 gctgcgtctg attgactgcg gccacaggcg tgctatcagg acaaatgatt cgcggctttg
    56761 accgccggcc gggagctgct ctcgcgggat tgctcgcctt gttgctattg acggcaacgg
    56821 cggcggcggg caatcactcc tggcatcacg cccggcatca cgatggagac gggcatagcc
    56881 atttctgcct catctgttcc ctggcccagg ggcagatcac ggccgcgccg ctggcctcca
    56941 tttccgcgat catccttcct ggctgctatt gggtcgtttc ccgggccagc gtcctccggg
    57001 tggccgcctt tcgctaccgc tttcctcacg gccgcgctcc gccctgcgcc tgactgcgtc
    57061 aggactggtt cgttgtttta gaaacccctt ggaagaccgt atgcgccgct ggtcagacgg
    57121 cggcgtcagc ttgagcgtaa acatgaaagc ggagtattca atttggtttg gtgcatcctt
    57181 taaagttcat ccccggcccc ggcggcaaaa tcttgtccgg ccgcggcccc gagccgcgct
    57241 gccggctctg gccatccttc ttacgctcct cgcctttggc gggggccagc cgctgctggc
    57301 ccaggcggct gacagcgtgg cagagctgaa gcaacaactg cggcaactac aggaaaactt
    57361 cgagcgcatg cagcgcgagc agcgcgcgca aatcgaggcg ctgacccgca agcttgatca
    57421 cctggcaaag caggcggccg ccgcaggcaa cgcacaacac cccgagcatg caccggttgc
    57481 cgccccgcag gaaagccccc ccggcgtccc ggcctcgccg gaagcatcca cgcccccggc
    57541 cgcctggtcg cccgcgcacc cgctgaccct cgcccgcacc gattcggccc acttggacat
    57601 cagcttcgac gtgctggcaa acttcggcgg ttcaaccgct tccaggcccg ccgactgcct
    57661 ccaactggga catcacgacc ccaaccagcg cggcttttcg ctgtccgctg ccgacatcgc
    57721 ctttgacggg gtggtggacc cttacttcga aggcttcgtc aacatttgcc tgagcctcga
    57781 cggtgaaaat gaaacccacg ttgacctgga agaagcctat ctgcaaacca tctccttgcc
    57841 ggccgggctg caagccaaag cgggccaatt ctacgccggt tttggccgcc aaaacccgca
    57901 acatgcgcac gaatgggcat ttgtggacag cccggtcatc ctgacgcgcg ctttcggccc
    57961 ggaagggttg cgcaacgtcg gcgcccaggt ttcctggtcc gcgcccacgc cctttttcac
    58021 cgaggcgatg ctgggcgtct tcaatccgca gggggaaacc gccttcagct tccgcaaccc
    58081 tgaggatccc catggccgcg cgtccttcaa acaaaacctg cgcggcccgg gcgacctgct
    58141 atacgtcccc cggctggcat cgtcattcga tctcacggaa cagcagacgc tggcggccgg
    58201 gctctccgcc gcttttgggc ccaatgcgac cggcgccggc ctccgcacgc aaatctacgg
    58261 cctggacctc tattggaaat ggaaggccgc cggggccccg gcgggctttc cctttgtctc
    58321 ctggcagacg gaagcgctct accaacgcta cgcggccggc gccgaccccg ccccggctct
    58381 ccccgccgag acgctgcatg attggggctt ttactcacag gtgctgtggg gcttcaaacc
    58441 gcgctgggtt gcaggcttgc gcggcgaata cgccacgggc aaccgcggcg ccggggattc
    58501 ccaggacgtt ttccggggtg aacgcacgcg cctctcgccg ggcctgacct tctattccag
    58561 cgagttttcc aaaatccggc tgcagtataa ctatgaccgg ggaaacttat tcggcaccga
    58621 acactccgtc tggctgcaag tcgagttttt gctcggggcg cacggggcgc acaagttctg
    58681 aaggcaaccg gcccttgccc aaaccctgcc gcccttccgg aagtgtctaa tcgggaaaaa
    58741 gccttcagca ggggatggta atttagaaag tagccccggg cagccctcct cgaataaagt
    58801 tccagtggaa tctgggcggg cctgaccgcc caaa
//
LOCUS       DAOPQG010000005        58089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_698_length_58089_cov_15.116742, whole genome shotgun sequence.
ACCESSION   DAOPQG010000005 DAOPQG010000000
VERSION     DAOPQG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 58089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58089
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_698_length_58089_cov_15.116742"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1504..1728
                     /locus_tag="PKI07_01000"
     CDS             1504..1728
                     /locus_tag="PKI07_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOC49657.1"
                     /translation="MNYSAIITIEAGKRSGKPCIRGLRITVQDILEYLAGGMTEDEIL
                     HDFSELTQDDIKACLAFAADRERKLFVAPA"
     gene            1725..2060
                     /locus_tag="PKI07_01005"
     CDS             1725..2060
                     /locus_tag="PKI07_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOC49658.1"
                     /translation="MKLLLDENISDRVVPQIADLFPGSTHIKSVGLKEAGDSVIWDWA
                     QQHGFAIASKDTDFYPRAIVFGHPPKFIWLRVGNCPTSLITNLLRSRYEIIRQFIESE
                     TESLLILER"
     gene            2635..2844
                     /locus_tag="PKI07_01010"
     CDS             2635..2844
                     /locus_tag="PKI07_01010"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOC49659.1"
                     /translation="MKYLLSSPDPARIGLAQSLLDAAGIEWEVRNEAVSQTAIGMPFA
                     TELWVLRDDDYQAARDLLKSGAARA"
     gene            complement(2925..3185)
                     /locus_tag="PKI07_01015"
     CDS             complement(2925..3185)
                     /locus_tag="PKI07_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49660.1"
                     /translation="MRQDVISVPISGNVAPANVAPATCFLQNLRPLGSIEAMLSRSPI
                     SIFHLLAQTAQLNDSMPQIGQARRVIFLHFAPRVANTLRQAR"
     gene            4217..5428
                     /locus_tag="PKI07_01020"
     CDS             4217..5428
                     /locus_tag="PKI07_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49661.1"
                     /translation="MSKPFETEATAPGIRVNPGQISILIATRARPEILAGVFASLNAH
                     TTRKELVSLWLYVDEDDSITRQAIAAKQLPEMDLAVHWHVGPRTACLGDTHDALWAAS
                     GRCAEIYMVSGDDMRFDTPGWDEVARKAFAAYPDGVLLAFPHDPMTADTATYPLFGWG
                     WLAELRRFFPGYFPYWYDDRWVSQVARLAGRYVKLPILLYPFRGRGRTRRMRNLPFWA
                     RFFQLMLEERKEDARRLIRAVHAKDAAAREAALGKVEEIAAALIREEGSFSDLYGVFQ
                     EERHSDLSPAERRAFDPQYVGREAFAITRLVSLAQQQFQAGRFAEAMEYLDATHLSDL
                     RLRQAQLLKVECLRKLGRDAEAGRLEKEALAAWPEHSAARRLFRFLGMVANDGRRMLV
                     GLTEKGRRGTG"
     gene            complement(5441..6202)
                     /locus_tag="PKI07_01025"
     CDS             complement(5441..6202)
                     /locus_tag="PKI07_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOC49662.1"
                     /translation="MSKASLAAIVRHCDRVLQSARVQDYERAANGLQVENRGTITRIA
                     AAVDASLATARLAVAARADLLLVHHGLFWSPAHPWTGPRYQLLRCLLEHDLAVYSSHL
                     PLDAHPRLGNNARLCAALGFRKLRPFYFDHGLHLGFQTQTRLPRANLAERLRQATGRP
                     PRLIPAGPAVCRRIGVVSGGAGDGLKQAAAEGVDTFITGEGPHWTYALAEELGVNVLY
                     GGHYATETFGVKALAAHLARRFNVPWVFLDHPTGL"
     gene            6470..7153
                     /locus_tag="PKI07_01030"
     CDS             6470..7153
                     /locus_tag="PKI07_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49663.1"
                     /translation="MSELVSKAELLRSLGRLVRGLSALFWGMPLALIVCVHTAKADSL
                     QPFGVVPPLAATALLVFGVWQLGDFQKQERVWGAALGRVRVFSLINFGLSPFLYWWNK
                     IPANPFFLVMVMLMALSALLFLASLNLAARRLSAMLPDEALRLETRQFTTFNLNLLLV
                     AFLLALLYIGLSVFSTLPLWLRMVGDVLERSSLWYLILLLLLPLALTMALLWKTKEVI
                     FESVFHAHP"
     gene            complement(7179..8270)
                     /gene="mqnC"
                     /locus_tag="PKI07_01035"
     CDS             complement(7179..8270)
                     /gene="mqnC"
                     /locus_tag="PKI07_01035"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOC49664.1"
                     /translation="MNHDPSLDDLRHRVWTGRRITPAEARRLYDLPLAELGALADRRR
                     QIARAAAYDGRGNQIVTYIIDRNINYTNICNVGCAFCAFYRAERAPDAYVITRAELDQ
                     KIAETLALGGTQILLQGGNHPRLGKQWYLDLLAHIRDRFPQINIHGFSPSEFVHFQKL
                     FNEPLEQILADFKAAGLGSLPGGGGEILVDRVRRQVSPRKAMTDDWLAVMDAAHRLGL
                     SSSATMMFGHVETRADRIEHLERVRAQQDRSGGFTAFIGWTFQAAHTRLRTPMAGAQE
                     YLRTQALARIYLDNFPSVQSSWVTQGPAIGQVALKFGANDLGSIMIEENVVAQAGTAF
                     RMTVAEMRRLIADLGYEPHQRDNWYRLLN"
     gene            complement(8283..9968)
                     /gene="recN"
                     /locus_tag="PKI07_01040"
     CDS             complement(8283..9968)
                     /gene="recN"
                     /locus_tag="PKI07_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOC49665.1"
                     /translation="MLTTLRIKNLALVADLTLELQPGYNVITGETGAGKSILIGALNL
                     ALGERADRTLIRGGCESCTVEAVFDVARLPAPLPAFLAENGLEPCADHQLLLKRTFTA
                     AGANRQFINGSPTTLATLATLGEWLVDIHGPHEHQSLLHPARQLAILDAFGGLEQPRA
                     AFAELVRRRAALETEKAALIVDEQTHARQLDLLRFQADEIAAARLHPEEEAQVEQDYR
                     RASNAARLLQLSQTALDLLSENETCLLTQAGLLGRTLQELQRLDAAAAPLLALHEQTA
                     AGLRDLQAELSRYAGKADIDPAALPPLEERLNLLHSLKRKYGATLAEVIAFGAEARRK
                     LQTLEQRTAELDRLNTALQQLTAGLWRAGQALSAARRKVIPPLTQAAGRQLADLGFRQ
                     SRLDIAFTSLDEAAFARQPGRTPSQGFDTLEFLFAPNPGEPARPLRAIASSGELARVM
                     LALKTVLAAADQIPVLVFDEVDANIGGETANAVGEKMRQIARQRQVLCITHLPQVAAP
                     AAAHYVAAKQVQGGRTISTLTRLEPAARVTELARMFGGQTDAARKHAAALLKG"
     gene            10160..13756
                     /gene="mfd"
                     /locus_tag="PKI07_01045"
     CDS             10160..13756
                     /gene="mfd"
                     /locus_tag="PKI07_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOC49666.1"
                     /translation="MLETPAVRSLARRLEQGGVLSLAGISAGAQPFLAAVVRQLFPRR
                     PLVFITDGLRSQESAQQDLTTWLAAETEAHGAEAAPADPPAPRSAPLFYPAWEVLPHE
                     ARLPHVDVISERLETLVALLSPASPGVASSPLIVTRVAALLQRTFAAATLRAATRTLA
                     RGDRLDPLEVVEWLEARGYEPEARVTQKGELALRGGILDVYPLTSPWPVRLEFFGDEL
                     ESLRQFDPLTQVSREEIQTVALAPAGELGLLRQSRSETASPAASSPPPAPGILLDYLP
                     PETVLILCEPEALAERAEEYAAQVPAADPLFLPWAGFLEQARARGLTLLEVREADFPR
                     SPAPDPAAPPVALRCGSLDPFRPLVDRAPEPAVAETQRREFFAQLHRWLRQGWAVHVF
                     CNNTGERQRFGELWEEYGLAADGVPACPASAGAAAPEPAGGPAPGFKLRAHLGSLARG
                     FLCDDARLAVVTDAEIFGRYKVQRPRRLKSPHAQAARSALDIDFTELEEGDYVVHVQH
                     GIGRYLGLQVPPPGAGTRPINGPGVAARARPECLVLEYAPNEPGQQPPRLFVPVTEAH
                     LVSKYVGAGKARPPLNTLGGTRWAHAKARAEQAVRDVASDLLAIQAARESLPGHAFAR
                     DVPWQREFESAFLFEETPDQARAIAETKADLERPKPMDRLICGDVGFGKTEVGIRAAF
                     KAVMGGKQVAVLVPTTVLAQQHFNTFRERMADYPIRLELLSRFRSRRAQQQAVRGLAT
                     GAVDIVIGTHRLLQKDIRFKDLGLVVIDEEQRFGVMHKEKFKMLRHLVDVLTLSATPI
                     PRTLYLALTGARDLSTIQTPPHDRLPVETIVAPYDERLIREAIQRELNRGGQVFFLHN
                     RVLTINTMAARLQALLPQARIVVGHGQLRSDDLEEVMARFVNGEADVLLSTTIIESGL
                     DIPNANTIIIDRADRFGLSDLYQLRGRVGRYKHQAYAYLLLPRHGGLLADVRKRLGAI
                     KQYSTLGSGFKIALRDLEIRGAGNLLGAEQSGHITAVGFELYCQLLKQSIASLKGQKA
                     PPRLEVAVRLDFLALGPADETAPPARASAQPLSGNPAPSAAHFACIPFRYIADSRQRI
                     EIYRKLAQVTDPAGLTRLAQELRDRFGPVPPPLALLLQLAELKVLARDLGISAIEVQD
                     DKLMLTRNADYIMSGSKFPRLTRKQPSARLKEIKKFLLTLAATASPAPLPGA"
     gene            13958..16078
                     /locus_tag="PKI07_01050"
     CDS             13958..16078
                     /locus_tag="PKI07_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49667.1"
                     /translation="MFSARQLFALPRMIGRGLAGLGLLLLVSAALGGGEIPPPVISAA
                     SNLEAQHIYVCMLYSPDRSGVAGLTNYLKQIGARGSGALWTYTHPLLNQMTVIHVVTD
                     FEGIKRALYTEGANVLILGHSNFGLGGVFATAKELSAQQIKTLYYMDDDRLMVWSSPW
                     VSVNVPRIRWHQSFPNWWPDFQDGASAIMPLDFGDPRGAPPFNYYLTYQLPGDPIHYK
                     IESVANSALERFPSSGKPAWYSPDGSLPDPANPAHRQYFLTATNTAFETVGIWVGTNF
                     QSGYYGQDYLCVPGGTGLCQAGWFFSIPAAGNYTLSATWPGGPLNTTGASYIVAHAGG
                     TTTVKKNQKLNGGKWIKLGVFAFAAGEYAVVLTDKASSQSLVIADAIRITSVANPGAF
                     DQVVDNGPCPPTHYGKRTVLFRRGLDIDPDQMSYRRLFYEGCNSGFYYLDTFRRGVVF
                     YTLHDSDGSGAATYLRSCLEGRSDEETWAALQRIQAIYDYYDFTKPPSEQGAAGAAAV
                     AAPPASAGSQKQRVAELSQSSAAQAFAGLQEEEFISDEGSAVQMVAGAFGRRRAEGIA
                     LALKQLAVPGTGGAAARAPRGTRGFVAARRILAAFPDESVPRLLELYDRGNAITRGNV
                     VRAAGGMPGDARVRDFLRAALEDKNVCEAASPEELGEPLRVCDVAYNQLVVGLGVKGV
                     LRTIGPIHSLAAREYHIGVLKTRL"
     gene            complement(16245..17432)
                     /locus_tag="PKI07_01055"
     CDS             complement(16245..17432)
                     /locus_tag="PKI07_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOC49668.1"
                     /translation="MKNPFPRVKIGLYALALTVGTSALAAEQPIIRNISRVPWLTIES
                     KVGYTNQIQYTNKLTGNHWAVLTNLVVTQSPYWFFDVSAASEPQRYYQVVNIAPTNEP
                     PPPPAPAAGMVAIPEGVFLMGVPGSTAGVALEHSVFVNAFLMDSNLVSYTLWANVYQW
                     AISHGYSFSHAGAGKAATHPVQKVDWYDVVKWCNARSEKDGLTPCYYVSTSLNAADVY
                     RTGVYNLGNGHVNWAANGYRLPTEAEWERAARGGTNGRLFPWATDKISKTRANYFGNT
                     ASYIYDEGPDGFHSSYSTGSEPYTSPVGDLKSGRNAYGLYDMAGNVGQWCWDWYGRSY
                     YTIINNPVDNPQGPNTGTARVVRSGSWQALAPAQKCSARDNRAPKTALNTIGFRCVRG
                     LDY"
     gene            17687..19018
                     /locus_tag="PKI07_01060"
     CDS             17687..19018
                     /locus_tag="PKI07_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOC49669.1"
                     /translation="MMKQYAVGFLTLMAGLAPMVGRGQVGEPAPPLMIKEWIQGPPVE
                     VKSGTNLYVIQIFTTTSLTNQQSLTNLNALQTRFKGQGVLVVGVSDEPVEKIREFMAH
                     DGKVIKHAIAADDRRKTALSYMRAIRQRGVPHVFVVGKDGQMLWHGHPQDGVEEVLEQ
                     VVAGRYDPAPAARLDVAHIQIDQYLTLARQNDKRVPAAGRQFLILWTNDVASLCDLTL
                     AITADRAPRKRDLELAAEALARAEELVATNSARVAEAQASYLFATGKKAEGLARAKEA
                     VAFAQGPREKARAEAFVHHLETRRADDVRKQVDQYLLLARRNDPEAAAAGQRLLVGLT
                     NDVAPLCYLAVQIVSDPRITRRDLPLANDALNQAEKLAPTNSTRLAVSRAIVLFETGK
                     EQDGLARAREALATAQGEKDKALAEKCLRTMTAAFESARTNQAKTNLPPSK"
     assembly_gap    19796..19895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19896..21272
                     /locus_tag="PKI07_01065"
     CDS             <19896..21272
                     /locus_tag="PKI07_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49670.1"
                     /translation="NVKEGVITFALTETYQTSVSTSTKANQGSWSQQPGYYKTAQRKV
                     TERTILDCIGQVLHGSPNYYSRTGTSSKPAARLILTQGELGGFFNIVPELADADTEFS
                     AEGYNSGYAEDAGLASTIEGTFVRLATGRHFLAVPEGYETEGNWPPGHHQPWGQIFVK
                     DAQRNLCDNVTFFFAITVQECYDCFYLNSFVTDTKFVLKKGSEQQEGPPCCSTYIEST
                     LTGSGIDRYYMSLSFDNTFNNPYLNDDNEAWIGVSDGPYPGVRGYLKDQLTGDGTTPD
                     QLPYVDRIISNQGTPAWYAMRFTLNGILTYKWKLAKFNANDLVLDFIQNGAAATYVAN
                     GYSFHALYCGLLTGSMKINETLVNSGKAFCCDNIWWDDSWYGVGWNSWQYPWSDYWES
                     PVNVPASLSLHVGVNEFYQPRWQWPQSSPEPNPALPDETVENLRGAGETYIPGTRWYF
                     LEGGWPQE"
     gene            21390..22091
                     /locus_tag="PKI07_01070"
     CDS             21390..22091
                     /locus_tag="PKI07_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49671.1"
                     /translation="MRPKIVILILVVAVGLVALAAVFKGIINRPKTPEPGDAAAVPTA
                     TNSSTGTALAPTPGSSPESKGESPSGDEIERELQEITDLSLAGSGNPHTAGLLAGKLT
                     HREKEVRAAALDALIHLNDTNIVPTLEQTVGVIEDPREKVALLDAIAYLKLPEDAEPP
                     PVVSKDAPEESIPPSSVVRSKGESGQDKQRMSTPKSPRRVPGKTRRSAIPPAAGAQQL
                     APPAPAPDAAPPPPQ"
     gene            22221..23609
                     /locus_tag="PKI07_01075"
     CDS             22221..23609
                     /locus_tag="PKI07_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HOC49672.1"
                     /translation="MNETHGSTNSAVPAASVTPAWVYWVRGLLGVALVGAGYLAWVSI
                     HNGPVAGCGPTSGCGQVLQSPWAYWLDIPVSVPALLVYLALLGTTVLLQKRPAPDEER
                     GAWAAIIALAVIVAGAALWFVGLQIFVIKAYCKFCMIAHACGLAAALLCLRHIPLARD
                     PATPMWATGSGLRGVPRAGVASVTAIGLAGVMALAGGQLLVPRKLNVVRDLRPAGRPV
                     AGQTNAINGTPGEGNSAAPTPASPQPHLVAPRQLSLYSNAFLLQLDTVPMIGSPDATN
                     VIVYVFDYTCSHCRALHPILIRAQRRFGPQLGIVSLPMPISTNCNPFLPPQSHSIANA
                     CEYARLGLAVWRAKPAVHGQFEEWMFAGARPAPVAQAKEYAAQLVGPDVLEAALKDPW
                     IEQQLLLDCRLHHINWMVSEQPVMPQLIIGDAISTGPLNSVEHLVLLLNHYLGLTAPP
                     PPVSGGQGEASF"
     gene            23639..24733
                     /locus_tag="PKI07_01080"
     CDS             23639..24733
                     /locus_tag="PKI07_01080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC49673.1"
                     /translation="MKTCSALLTAALLLSGSVRAASPAPTNAAPASPVSATATNKVVA
                     RGKGWEITRGELGKGVTAAMAQVAARGRQVTTAQVPEVERQVLAQLINLRLVLARATR
                     ADREAGKAAANKRYATARANAASEEAFKLQLKFLVTTPEELLAKWTDALTSEAVMKRE
                     LKIAISDAEARKYYNENPKQFDLPEQVRASHILIATSDPRTGAGLSDEEKAGKRKKAE
                     AVLQQARAGEDFGKLALAFSNDAGSRARGGEVRFARGEMPAEIEAVAFSLKTNQISDL
                     ITTTNGYHIIKLIQKIPARKLKYADAAADIKNGLTQAAIEEQFPAYIARLRDAANVEI
                     LEEQLQPQDPARTGLSLPGPGAPPKPAGVK"
     gene            24862..26541
                     /locus_tag="PKI07_01085"
     CDS             24862..26541
                     /locus_tag="PKI07_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC49674.1"
                     /translation="MPLKVVVIGGVAAGPKAAAKIMRLDPTAEVTLLEKGQFISFAGC
                     GLPYYVSGVVKEQKDLMSTPIGVRRDPAFFQKVKNVKVLTETEALEIDRARKRIRARR
                     NGAAEDFWLDYDKLVLATGARPVIPPVPNATLANILTLHSVADADAIKARLARNHGRD
                     VVMIGGGLIGVETVEALVQCGCRVTVVERLPQILRMLDWEMARLVEQHMEAHGVQVMT
                     HTEVQAFEGPGQVTGVVTNRGRLPADLVVLGIGVKPNVELARQAGLEIGPTGALRVDA
                     GLRTSDPDIYAAGDCAESTDLITRQPCYVPMGSTANKQGRVAAINVCGGEDRFPGVLG
                     STVCQVFDFCVARTGLTEAAARQSGYAPVTVLVPDADRAHYMPTARPVILKLVVDERT
                     RKLLGAQAVGPGAGDKRMDVAAMAITAGMTVDQLSKVDLCYAPPYSPALDNLILAANV
                     ARNKLDGLLHGLTASEVQQRLEARADFVLLDVRSPAEHQQERLPGSTFIPLGTLRARL
                     DELPRDKEIVTFCKASLRGYEAARILQGAGFPNVRVMDGGLAAWPFAKLSG"
     gene            complement(26597..27718)
                     /locus_tag="PKI07_01090"
     CDS             complement(26597..27718)
                     /locus_tag="PKI07_01090"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC49675.1"
                     /translation="MLCLLGLLFGALDGPAASTPDFSPAAAILQRGIKERAFPGCTVV
                     VGTADKVLWSAAFGRLDYAGGAPVTTQTLYDLASVTKVAGTTAVFMQLVQRGKVRITD
                     PVSRFIPEFLALAPSAEDKAWRQRVTLEHLLTHTAGLTAWKPYYRSHTNYAAMLRAIL
                     TTPLESEPGARTRYSDLGLFLAGEAAARAGGKPLAALERELVFGPLGMTNTLRNPAPG
                     LRPRIAPTEKLPDQERFAHGVVHDENARAGEGLTGHAGLFATAEDLGKLAAELLRADA
                     GQSQWLTRAVLAEFARPHNLAPDSGRGLGWGIERGAKRVLSHNGFTGTYLRIELSRKM
                     YLVLLTNAVHPTRDNHQLGAIRREAIQAILEAVDKSGGV"
     gene            complement(27960..30050)
                     /locus_tag="PKI07_01095"
     CDS             complement(27960..30050)
                     /locus_tag="PKI07_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOC49676.1"
                     /translation="MKEYQAVDLRNLAIVGHASCGKTMLSEAMLACAGVINRMGTIPA
                     GTTVSDYHESEQQRLISVSTTLMHLEWQGKKFNVLDCPGYADFISEALGALRVSDQAV
                     VVVHANHGPGVGTDTVWKSATANGIPKVIVVNAFDKEETHFDATLAQLREHFGERVLP
                     LTLPVNAGPGFNQLLDVLRQKVMTYAGDKSGKYTEAPPAGALAEKASQLHNQLVEYVA
                     ESDDALMEKFFAEGGLSEAEMRAGLHAAFQKQVFTPLFCTAAENNVGVAQLMDFLAQY
                     GASPADRPAVEAADADGNPVPVKLTDPEPVLYIFKTMSEAQFGELSFFRVYAGAVKFG
                     SEVYNADRRSTEKIGQIYQLNGKTRTSVPALVAGDIGAVVKLKDTHTGNTLYGGKKLY
                     SLPKVEYPKPSIHAALKSSAKGEEDKVASGLAALHHEDPAFQYVVDSELRQTIVSAQG
                     ELHLEVIAESLRRRYNVHVELIEPRVRYRETIKGRGDSKYRHKKQTGGAGQFAEVWMR
                     IEPKPRDTGVEFTQTLTGQNVDRVFVPSVEKGVMKACEEGILAGYRVVDVKIEFYDGK
                     MHPVDSKDIAFQIAGYFGFKESFEKARPCLLEPIHLVEIRIPDDCMGKVMGDLSSRRG
                     KIQGMDMEGGIQLIKAHVPAKELYRYSSTLRSLTGGRGAHSESFSHYEEMPREAEQKI
                     IEESKRAKQAQQAE"
     gene            30245..30330
                     /locus_tag="PKI07_01100"
     tRNA            30245..30330
                     /locus_tag="PKI07_01100"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30279..30281,aa:Tyr,seq:gta)
     gene            30462..31310
                     /locus_tag="PKI07_01105"
     CDS             30462..31310
                     /locus_tag="PKI07_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOC49677.1"
                     /translation="MQIIRYEDPQGAVRYAARQPDGSARQIAGDILGAYEVTPTPARV
                     KKLLAPVAPVALFCIGLNYRRHAEEGKAALPAFPVLFMKSPAAVQNPGDPIELPRHLR
                     SDKVDFECELAVVIGRRAKNVSRADALDYVLGYTCANDVSARDWQGPWGGSQWCRGKT
                     FDTFAPLGPCLVLKDEIPDPAALKLRTLLNGQVMQDWRCDDMLFDVPTLISFLSGSTT
                     LLPGTVILTGTPHGVGFARKPPVFLQPGDRVTIEIDRIGALTNPVVEEGGEFPPPRAP
                     RRDPAG"
     gene            complement(31307..33754)
                     /locus_tag="PKI07_01110"
     CDS             complement(31307..33754)
                     /locus_tag="PKI07_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49678.1"
                     /translation="MRCLLSYLLVGLSVLALGGRVAAPAAAPKTAGQVWPPDPENKVV
                     CISGIYPHLAVFNPYGECGIGAVAPWADKLWFITYPPHAPEGSQDKLYTVDSRLKLEM
                     RPESVGGTHANRMIHPESRQLILGPHFIDEAGRVRTVDLARMPLRITGNARHLVAPAD
                     KVYFVGMERELYEVEVKTLAANRIYGLLGGPYPGAHGKGAATSAGRLVVANNGERGWR
                     YDGDPGFDGPAGCLAETDGRDWSQAWRVIERAPFTEVAGPGGLRGVAPGDDRLWALGW
                     DKRSVILKLREGGQWHTFRLPKGSYTQDALHGWYTEWPRIREVTEGRWLAHMHGLFYD
                     FPPTFGTAHYGGLRALSTYTRMPVDYCAWRGQIVMARDDASIMQNELAGQSHSALWFG
                     SWADLEGLGAPAGWGGPWLNDDLAAGAVSPPFLVAGFREGTLHLKHHSASAVDFTVEA
                     DAAGRGQWRPVAGVSVPAHGYAFRVLGRELKAGWVRVRAGQAARRASAYFHLGNPPRR
                     PDPARFAALADADYRGPVIDGLVKPDAGDARRLILAANLYEGARVKERRGWVMDGRLE
                     WRPLDDADKEAALRAPPGAGPALAVEAASVVVTHGGQRYRLPRTAAVYDDPFPTGWPR
                     ALREVVTERNLFNVHGTIYEVPRADAGGFQRLRPITTHHKRIRDFAAWRGLLVLTGVR
                     AGARPDGHCHPSADGAAAVWFGEVDDLWRLGAPAGVGGPWKDTAVAANEPSDPYLMAG
                     YRTKSLALSHRAAQPVAFTIEVDFAANGEWSRYCELVVPPGRTLKHRFPAGYSAHWVR
                     LKTDRAARASAIFTYRP"
     gene            34412..35788
                     /locus_tag="PKI07_01115"
     CDS             34412..35788
                     /locus_tag="PKI07_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOC49679.1"
                     /translation="MTLTEKVLARAAGKARVAAGDNIWVQADVLMTHDVCGPGTIGVF
                     HREFGKGAKVWDRHRVVIIPDHYIFTADSKSNRNVDILREFAREQGLVYFYDVIDDPN
                     GHWVFDASKGMLKRQYGSRYAGVCHTALPQKGHTRPGEVLFGTDSHTCMAGAFNEFAT
                     GIGNTDAGFVMGTGKLLVKVPETMHFRLEGKLPPGVMAKDIILHCIGEIGFDGATYRA
                     MQFDGPGAASLSMDDRMTVANMAIEAGGKNGIFEFDAQTQAYVDYRCKLNGTKAGYEP
                     VERDREEKFVYELVLDLSRLEPTVACHPNPGQRKLAKELGHIRLDRAYIGSCTGGKTS
                     DFLEFARVVQGRRVVIDTFGVPATPEIVHDLQTARWGEQTVWEILTAAGVLMTENAGC
                     AACLGGPVDTFGRMNAPLKCISATNRNFPGRMGHKDSQVFLASPATVAASAVTGRITD
                     PRELLPRA"
     gene            35788..37731
                     /locus_tag="PKI07_01120"
     CDS             35788..37731
                     /locus_tag="PKI07_01120"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC49680.1"
                     /translation="MRRETLDTWCERGILGLVLVVLIWGPLAMGAVRPSEFLVIQGAT
                     MGVLLLWGARLWLSPRPQVLWPPICWVVAAFVGYALIRYHYAEVEYLARQELIRLLIY
                     AFLFFALLNNLHRQESLQVISSTLITLAMVIAAYAVYQFLTRSDWVWRYRVGFDYGGS
                     GTYVSRNHLGGFLEMLLPLGLAYTLVSRFKPVSKVLTGYASLVILAGIVATVSRGTYL
                     STAVALGLFFGVLLFQRSHRLPAFLLLVVVLAAGAYLLPRNISVRLRFQQYLTEPGPD
                     PRAEDGKRSVFWDPEDARVILWQSALRVWEQNIWWGVGPAHYDVRFPAYRPLAMQLHP
                     YFAHNDYLQFLAEWGLVGTGLGAAALVLLGWGVVGSWRYVRGATSDLGHPRNSSKFAF
                     VLGAVMGLVAILVHSVVDFNLHIPANAILAVTLAALLSSCLRFASERHWQSLKWWGKA
                     LGSVLVLAGTVYLGQQGWRNAREYAWLQRADRAPLFTMAQVDCLKRAFAAEPMNAETA
                     YAIGEIYRVKSAEGGDDYQELAVQALEWFKRSLELNPWNAYGHLRYGWCLDWLGRFAE
                     SPPFFARAAQLDPNGFEVAANAGLHYAQAGNYAAARTWFERSLRLERKSNPIASSYLP
                     IVTSRLLEAATNEFSAGLRSASP"
     gene            37890..38687
                     /locus_tag="PKI07_01125"
     CDS             37890..38687
                     /locus_tag="PKI07_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOC49681.1"
                     /translation="MKTIQSLIYKLLAGGVVTAMAFTLAAQTADQGMAKVVRLKGAAR
                     YVSGSKEGQLLKVGDLIPPGTAIQTAEKSRVDLVLGEGSAPVGGPPAAGDMLTYTPTV
                     EQNVVQVWENTLLGIDKLNITRTAGDEVTETQLDLKAGHIFGMVKKLSAASKYEVKIP
                     NGVAGIRGTVYDISAEGVIKVLSGSVVLAYVGADGQVQTQVVMSLQEYDAPSNTLKPL
                     ADINKSGMEKCMHGWPGRPPPPRPPPPPPPPPPSRWKPGHGHHHGHH"
     gene            38777..41083
                     /locus_tag="PKI07_01130"
     CDS             38777..41083
                     /locus_tag="PKI07_01130"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOC49682.1"
                     /translation="MKRLPIKRVPALITLGVIVLISLVRGWQFNFLESLERMTYDLRV
                     RAALRHAAPLATNLGFVFIDDASIAFVRTNTTLGYNYGLYWPRQVYGRLVQELAAQGA
                     RAVALDVIFHELRPDHPPVRMADGSFPESDEFFALQMRRAGNVILALPEDLTLPALFQ
                     TNALALGDISTHKDHPEGVLRRAQAFRVRRHWHFAFQQVAADPEFGVDLRRARVEGRQ
                     VVLPRSGVEDIRVPLDAEGCFDLADFAGDKLPPGVARRAKPFTEERIWHMGIVLAAVE
                     LGLDLAGAEVRLDQGRITLQGAHGVRRDIPVDADGYFYINWALPPNHPQLAQESIQAL
                     LQQDGLRLQQRTNELHNHWRGKLVVVGSSATGNDLTDRGATPLKADTLLASQHWNVAN
                     SLLTGRFVRRAPLGVEWALVALLAVVAAVVTWKMRVLVASALLVLLGAAYVGLGFVLY
                     ARTCYWLPLVLPVAGSLLLTHLGLVTWRVVFEQAERRRVRSIFARIVSPKIVNELLAA
                     ESFSLGGVRREITVFFADVRGFTELTDSSQERVAALVRRHGLTGQAAEDCFDEQARET
                     LSTINLYLGLVADTVIRHDGTLDKFIGDCVMAFWGAPTPNPRHALACVRAAIEMQRAV
                     EQLNQQRAAENARRERENVERASAGLAEKPRLPRLLLGTGINTGMATVGLMGSAAELQ
                     NYTVFGREVNLASRLENASGQGRIFIGQCTYEHLRRDDPALAATCTELPPQDLKGFRS
                     PVKVYEVPWRSAEAVPSAAGLARQDSSA"
     gene            41200..41688
                     /gene="coaD"
                     /locus_tag="PKI07_01135"
     CDS             41200..41688
                     /gene="coaD"
                     /locus_tag="PKI07_01135"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOC49683.1"
                     /translation="MRTAIYPGSFDPVTNGHLDVLQRATKLFDQVIVAVARSESKRPL
                     FSLEERVRMVVRATQHLPNVEVDSFESLLLTYAERRSAQAVVRGLRAVSDFEFEFQLA
                     LMNRKLNDRIETIFMMPKDTYTFLSSRLVKEMAQLGGDVSAFVPAHVREALAARFSSR
                     PA"
     gene            41699..42187
                     /locus_tag="PKI07_01140"
     CDS             41699..42187
                     /locus_tag="PKI07_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HOC49684.1"
                     /translation="MPLVVNLRHLEAHNVRLQGQLSVAELDIDTRDEVIQVAHPLDYA
                     LEVQKLENGVLAQGRLSLKLECQCVRCLKSFQYPLELKEWTCHLPLQGEDCVAVVNDC
                     VDLTSFVREDMLLEFPRHPLCDPGCRGLPGISRGKAKNTRRPGESERDQSVWAELDKL
                     KF"
     gene            42194..42382
                     /gene="rpmF"
                     /locus_tag="PKI07_01145"
     CDS             42194..42382
                     /gene="rpmF"
                     /locus_tag="PKI07_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOC49685.1"
                     /translation="MGVPKRKPSRSRQRMRRAYNSAMTLPQLSTCPQCAAPYVPHRVC
                     PACGYYKGRQVITVTAGA"
     gene            42428..43477
                     /gene="plsX"
                     /locus_tag="PKI07_01150"
     CDS             42428..43477
                     /gene="plsX"
                     /locus_tag="PKI07_01150"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOC49686.1"
                     /translation="MRIAVDVMGGDHGCGVVIAGVKRTLQADSSISAIYLVGNRSEIH
                     AALPPRGFRDHRVRVVHASEVLTMADKPAAALRRKKDCSIARAIELAREGKVDAIVSL
                     GNTGGIFAAATFKLGRIPGVDRGGIATVIPTPEHAFVLLDSGANIECKPLHLAHYAVM
                     GSIYAREILGYKNPRVGILSIGTEDSKGNELTREAFRLCKLLDLNFIGNVEGHDLFNN
                     RVDVVVCDGFVGNIVLKTCESLASAMFAMLKRELTANPRRQLGALLAHNAFRTIKRRM
                     DPEGSGGAPLLGFNGTVMKAHGSARERAIASAIRVTATNVQHHVNQIIAREIARVNER
                     LAADEATAAVLQAQN"
     gene            43622..44659
                     /locus_tag="PKI07_01155"
     CDS             43622..44659
                     /locus_tag="PKI07_01155"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOC49687.1"
                     /translation="MNPSTPPSFKNPRAKYNFQGRTCSITGVGSYVPSRVLTNAELEK
                     MVDTSDEWITTRTGIKERRIAGPNEFTSDLGAQAALRALQHAGVSPEEVELIIVATIT
                     PDMPFPATACLVQQKIGAHRAAAFDLEAACSGFIYGLEVAQQFITSRTYDTVLVIGAE
                     KLSTIVDWKDRNTCVLFGDGAGAVVLQNRPNSHGLLTAVMGADGRKADLLFVAGGGSR
                     CPASAESIAAGMHYLRMEGRETYKSAVQAMETAAEEALRRCEIDISRIKCIIPHQANR
                     RIIDAVVERLGGKPEQLFVNLHKYGNTSAASVVIALDEAVASGRIQRGDLVLLVAFGG
                     GLTWGAAVIEW"
     gene            44729..45058
                     /locus_tag="PKI07_01160"
     CDS             44729..45058
                     /locus_tag="PKI07_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49688.1"
                     /translation="MSARRSANNGSATEVKVEAEQTCLTLSPDAVIAHKNGIEFRSSR
                     PFAVWREMTMSLQSAGGDKVCCSGVVISCTGNKHTGYHVSMVLTGLSRQAQARLSAMV
                     YAPFNSF"
     gene            45298..45441
                     /locus_tag="PKI07_01165"
     CDS             45298..45441
                     /locus_tag="PKI07_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49689.1"
                     /translation="MAALRPENNRREAALVLPDQNLVPVLFVLGFPSLFVILKAWKYF
                     SVF"
     gene            45438..46505
                     /locus_tag="PKI07_01170"
     CDS             45438..46505
                     /locus_tag="PKI07_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOC49690.1"
                     /translation="MKTAVDGGASDVHLKIGTPVIFRINRQLIAIECPFPTEEWMGNV
                     VQQITPPHLRKRVEEEREADFSYYVAGIGRFRTNLFQQRGQWCLAMRYVKTRVPSFEE
                     LGLLEQVRRIAESPRGIVLLAGSTGCGKSTTLAAMIEHINANFKKHIITLEDPIEYVF
                     EDNQCVIEQREVGLDTLSFHHALKHVLRQDPDIIMVGEMRDSVSFTSAMSAADTGHLV
                     LSTLHTTNASQSISRILDFFKADEREQIRRQLAGTMQAVICQRMVATLAGGMTPALEI
                     MINTPTVKKLIEENRLDKLAAAIETGTEDGMINFNQALFNLVKQGKISEQEALNKASN
                     PQALEMNFKGIFLDEGRRILA"
     gene            complement(46672..48195)
                     /locus_tag="PKI07_01175"
     CDS             complement(46672..48195)
                     /locus_tag="PKI07_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49691.1"
                     /translation="MSFPLKRILLLLLAAALLAGAGWTQRLINHDRDLLGLTQVQPLE
                     NAPPVLAFTTVALGGFRGLISNALWMRATDLQDQDKFFEMAQLADWITKLEPHFVHVW
                     LVQAWNMAYNISVKFKETSPGQFPDRWRWVKAGIELLRDDGLRFNPNETLLYRELGWF
                     FQHKLGHNLDDASSYYKQQWADEMARVFDKKTPNLDELIHPQTAEAQAQAKLLRETYK
                     MDPEFMKTVDEQYGPLEWRLPEAHAVYWAALGLQKARENPTRIKEDDLITLRRVIYQS
                     MLLSFHRGRLILNPFAQAYEFGPNLAIIPKVNFAYEQAAEEDQQNREHIKKAHRNFLR
                     DAVYFLYTYNRLADAAHWYKYLSTQYPNKPLLEGKPDSLPATLTLDQYAVARVQEDVS
                     DSGGRDRTQGIVEGLLVSSYRSLALGEDGPAAGFRLLAQRVWEAYQSQMPKQRLAAIG
                     LRPFAETDQEIRKLMLDPQGGEPAEVRAVLRSKLGEPPETNAVPAGTNAPPEPAVPR"
     gene            complement(48192..49571)
                     /locus_tag="PKI07_01180"
     CDS             complement(48192..49571)
                     /locus_tag="PKI07_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC49692.1"
                     /translation="MQPLLAIARLTWKAAFRFRLFLVVAGLLLGSVVGLPLLIKDDGT
                     ARGFTQILLTYTLSSITALLGLSTLWLACGTLARDVEECTLQVVATKPVARWQIWLGK
                     WLGIMSLNAALLALSGASVYGLLQWRATRLPVDEQKILRHEVLVARGSIKPPSFDRQI
                     AQEADRLLRERRQKYPDILANISEARKQILEQVKAGYEVVPPGYMHIWEVDLGNAGRI
                     APGQMVHLRIKFNAANKSASGTFEGRWLVGVPRKTSLWHSDAMSLAPDTFHEFEVPAY
                     YDEQGILTVSFVNLNETALLFPLEDGLEVLYRQGGFGLNYARGLGIIFCWMALLATLG
                     LASASLLSFPVAAFFSLAILTLALSSGTIASTVEEGTLGGFDSEHGQAGHSSVDVVAI
                     PVFRTVLAAINLAKDFSPIDSLSTGRSVAWGELGRAFGQIVLLLSGSIGLIGILIFTR
                     RELAAAQTQ"
     gene            complement(49603..50808)
                     /locus_tag="PKI07_01185"
     CDS             complement(49603..50808)
                     /locus_tag="PKI07_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC49693.1"
                     /translation="MSTTIADPGPRPATGEVVVSIRGLTKVFKDFWGRPKARAVDNVD
                     FDVRRGEVFGLLGPNGSGKSTTVKLILGLLHPTKGHLEVFGHSPSHVATKARIGYLPE
                     ESYLYRYLNSGETLDFFGNLFCLPRHDRHDRTEQLLEMVGLSQARLRAVGEFSKGMQR
                     RIGLAQALINDPDLVILDEPTSGLDPIGCREVKDLILALARRGKTVLLSSHLLADVED
                     VCDRVVIYYGGRIQAMGSLKELLAAPDAIRLTLPALPCETLERVLAFIRQDVAESRIS
                     IDTPTQNLERYFLEVVQNARLAEAETSGAVSGARVAAYLRGEAEAAPASDKILERLAA
                     PPPARPAPTPAPKPDETVDRQKLEALIKPSEPAAPPPPPPAAEKPSTTEELRQANEKL
                     ASLVRKQKE"
     gene            complement(50855..52858)
                     /locus_tag="PKI07_01190"
     CDS             complement(50855..52858)
                     /locus_tag="PKI07_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOC49694.1"
                     /translation="MQITSLLSRHEHDESCATCGHHHEHTPVRLWQTLVGLVFVFNAF
                     LVDWFFAQGHTVASASGLIGAVILGYPIVLTAIKDLRGGILSINELVAIAVLAAFATG
                     EYKTAGVVAFFMLMGEIIETRTAEGARASIEHLIKLTPTKARRITPQGEEEVAAKDLA
                     IGDVIRIRPGDNVAADGVILRGQGSFNQATITGESLPADKKPGDEVFAGTQNLTGVLE
                     IKVNRAGTDTTLGRVRELILAAEKTKLPIMKIVDQYMGFYTPLVLVIAALVWAFTRNL
                     DNVISVIIFGCPCAFILATPTAMVAALSAAARLGILIKNVADIELAAKLNAFVFDKTG
                     TLTTGELAVSRLAPLDGVTPAELLRVAASAERYSNHPTAKALMQLAAEASVPLTEPKD
                     FAETAGRGIKARVGDAQVLVGRAQWLHDNGVKGDFLQSVDLNETEGWSLIFVARDGRC
                     IGWVGLQDQTRAEARESLQELKASGVRRIAMISGDRQPVALRVGREIGCEEVRGECLP
                     QNKVDFVRSIKAKGYQVAVVGDGVNDAPALAAGDIGIAMGAAGSEVAIHSATIALMNN
                     DLRRLPFLVKLSRSTRAVINQNFLFGAVFIIGGMALSAMNYVPPVLAAAMHTAGSLIV
                     VFNSARLVRKGEELEHYQPEPAAPGPKQPAAPGTRPGLAPQMA"
     gene            complement(52901..53998)
                     /locus_tag="PKI07_01195"
     CDS             complement(52901..53998)
                     /locus_tag="PKI07_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HOC49695.1"
                     /translation="MNPHAHSHDRPPEPAPPPEMPLDAGAQALAEALRSSFVIVKVVM
                     VLLVLLFLASGFFTVGPQERAIILRFGKARGEGEKALLGPGLHWSFPYPIDESIKVSI
                     SGLQQVSSTVGWYAVTPEQLLAGIEPPAGATLNPIVDGYVLTADTNIIHSRATLTYRI
                     KDPIQYVFGFVAASNAVQSALDNGLLYAAARFNVDDILTRDIASFQEMVRRRTTQLLE
                     LQELGVVVEQCVVQSVPPRQLKDAFANVLKAEVMRSKLLNEARTYENQVTNKAGADAE
                     SLVNLAQSDRARLVSDTSAQAERFQEILAQYRRHPALFEEQRLTETLGRVLTNVQDKI
                     FLAEGAAGKPKELRLLLNREVPKPKPEEAKP"
     gene            complement(54006..54917)
                     /locus_tag="PKI07_01200"
     CDS             complement(54006..54917)
                     /locus_tag="PKI07_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOC49696.1"
                     /translation="MKRNSLTLVIGLVLILIFGLLLFTFQVRTTEVAVVTTFGKPTRP
                     LTEPNLYLKWPWPIQKVWSFDKRVQNFEDRLTEGLTQDNFNLLTSVYVGWQVSDPAAF
                     FPRFADSANPIGAAETLLDQWLGNAKTAVVGRHPLADFVSTSDGGASFVAIENEILAA
                     IQSQLRTNNLGLEIVFLGFKRLQLPESVTQSVFERMTSERQVLADRFQYEGEAEAQRI
                     RSDADRRAAEMLADAEGQATEIKGKGEAEAAKSLKVFQQNPNLARFIFRLSALEDSLK
                     ERSVLIFDQHTPPFDLFRGASTNLLQP"
     gene            complement(54923..56803)
                     /locus_tag="PKI07_01205"
     CDS             complement(54923..56803)
                     /locus_tag="PKI07_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HOC49697.1"
                     /translation="MDKTLQRNGLINLLALIVTGAAGVLAARYAGSLAGQVGALYLGL
                     GALVALVSWFQMRLEERERLEKLELDELARTRSASAMFEAKTAELFPAQRAREQFERF
                     FVPAFTVVLFAAQAGGAYFLWRWLSRSTTKIELKEPIAGLALFGLFALILFLLGRFSA
                     TVARLQRHRLLRPGASYLLCNAVLCLVVALGIVGVWAGFAQTDFYVARALCGLLGLIA
                     LETLVQLVLEIYRPRVRGKVGQPLYESRLVGLLGQPEGLVTTAAQAMDYQFGFKVSET
                     WFYRFFERALGWLILLQVGALLLSTCVVFIEAGEQGLLERFGRRVESRSPLEPGAHFK
                     WPWPIDRVYRFQTERIQSLTIGSLPDPAKENSRFVLWGQGHSVETNFLVANREQLSHT
                     AVTPAVGKRTPPVSLLTVSVPIYYQITNLVDWAYRNEDAPALLEHLSTREVLRYLVGV
                     DMGDIMSQGRGPAAQDLNERIQAAANERGLGVRIIAVALGDLHPPIKVAPEYEKVVAA
                     SQTKQAKILAARADDIRTNALTRGQTVSLTNQASAERWAREIRALAQAALFTNQIPAF
                     EAAPSVYTERAYLQTFVRATAPARKYLLLTTNTQDVITFDLQESIAQSLLNLSVPAPK
                     TQ"
     gene            complement(56810..57952)
                     /locus_tag="PKI07_01210"
     CDS             complement(56810..57952)
                     /locus_tag="PKI07_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOC49698.1"
                     /translation="MRQFVTIASNGFMELVRQPIFLLLMTCSAAFEIFLATPYYFAFG
                     DEPRLVKNSTLAVMLLAGLLGAVLSASASLAREIHTGTALAVLSKPVRRAQFLLAKYV
                     GLAAALAVLTYVNLVAALIASRMTFDSYGKTDLSAVGIFAAGVVAAYAVGGFSNFFLR
                     RPFVSDAVFALLITVTLAAFVIFQFTTQLRSVNEPGSVDWRLVPAGLLILFALWILAS
                     LALACSTRLDMVPTLAICTAAFLLGLMSDYLFGRRAEPVWRRDLAAELHSGRWTEKQK
                     VLLRETVAKYDVDHNDQLDLAERKRISQPDRARLRQAGLGGAWWASVLYTATPNWQLF
                     WLADALTEGKSTFHWGYAGKAFAYMAAYVGAALVLAVLLFEQRELS"
BASE COUNT        10414 a        17740 c        18993 g        10842 t
ORIGIN      
        1 accttgagtg gaaaccatga taaccaattt gctgaacttg acggacacaa ctggagtcca
       61 tgggatttta gctggtacac ccgacgggca tcccttgtac accccttgta cacagcgtag
      121 aagtagggaa aaatcaaggt atttggatga aggtgtgctc aagccggaat gatttgggtt
      181 ccatcccgat ttcgttcctg cctacaatca taaatcactg attttacagc cgaaaggaaa
      241 ggtggattcg acggttatat catggaaatc gccaactaaa aggtctaagg aggctacagt
      301 ctggacggcc attcccctcc agattccggg gaaatgacgt cacaccattg aattttccaa
      361 ctgaatcgct ccagtttaga gcaccattaa cgttaacgta tcttaaatct tcgcgccata
      421 cgcgaaaaat caggctaaat atactatttt catatcggcg cgcgggcctc cggcccgctt
      481 tcaatgttct actaacagca gcgggactac agtcagtaat tcggcaaatt gccattcccc
      541 acaacctcca aatcccgccc ggatgtaaaa acagccagaa ttgcttatgc tgaaggacca
      601 attcgactca ctttgttaat agaccgctga aagcgggcca gaggcccgcg cgccgacgga
      661 ttgcccttcc tcacgcctcc caaatcccac ccggatgcaa aatcagttag taatgcctgc
      721 gccaaggagc caatccaatc cgctttgctt atagaacact gaaagcggcc cagaaaccca
      781 cgtcagtaaa ccccggggtt ctcccctcaa aggaccgccg gcggccccgc acgggaggcg
      841 gagctcctgt tttacagcgg ccgggtcgcc ggggcggagg ctccaaagtg tccggtcgtt
      901 ccgagattat gttggggctc ggtagctccc ggggctgcgg cgctaatcag catgcgcagg
      961 acttttggca gttcattgat gaccacctgg ctgatgagaa cctgaacctt aagttgccgg
     1021 ccctttacaa gaacttcatg gatatcgggg ggccaggaac tacggcctga cccagcgggt
     1081 ggtcgtattg gtggccgaag tccagattga agcgggtcca tttatgggag gcagggcgct
     1141 ggggcgctgg cactcccgcc cgctcccgcc gccgctcaaa ggctgcccag gtttctctgg
     1201 caacctgcgg catgggatta tgaagggctc gcaccgcctt ctctcgggcg gagacgagcc
     1261 cccgtcgggc gagctccggc ctgtgcgcaa gctggaagac ctgttaaccg cgggcttgac
     1321 cgcagcaggc ccgcagcgta ccaatgcctc cgatgaagga cagcctgcca tccgcggagg
     1381 ctgggcgttt ggataacacc cccaaacggg atgttatcca gacctgtata atcacgggtt
     1441 cggcggcttg cgcatcggca gcgagacatt gaccggcctg gactccggac atagcattgg
     1501 tacatgaact attcggcgat catcacgatt gaggcaggca agcgtagcgg caaaccgtgt
     1561 attcgcggcc tgcgcatcac cgttcaagac atcctggaat atctggcggg cggcatgacc
     1621 gaggatgaga ttctccacga tttttccgag ctcacgcagg acgacattaa ggcgtgcttg
     1681 gcgttcgccg cagatcgcga gcggaagctt ttcgtggccc ccgcatgaag ctgcttctgg
     1741 atgagaatat ctcggacagg gtcgtgccgc agatcgcgga cttgtttccc ggctccacgc
     1801 acatcaagtc ggtcggcctc aaggaggccg gcgacagcgt catttgggat tgggcccaac
     1861 agcatggatt tgcgattgcc tccaaagata cggatttcta ccccagagct attgtgttcg
     1921 gccatccgcc caagttcatt tggctgcgcg tcggcaattg ccccacgagc ttgatcacaa
     1981 atcttctgag atcgcgctat gagattattc gccagtttat cgagagcgag accgagagct
     2041 tgcttatcct tgaacgatag gaagccgaat ccctatcaag cccgcggcgt caataggcaa
     2101 tagtaatttt tgcgggagcc ttttctgctc ccgctgggcg gaactcgagg cgggaactcc
     2161 ggcttggttt tccgtggggc agctcggccg gaggagacca gctaaaccct gattcgggcg
     2221 caatcgggcc cgggtggcgg cccgggtcag tttgatggcc aggacggtgt tggtcttgaa
     2281 ctggttgccg ttcattttgg cctccagggc tgggccgctg gaactcccgc ccgctcccgc
     2341 cgtcgctcaa tggctaccca ggtttctctg gcaacctgcg gcattggatt atgaagggct
     2401 cgcaccgcct tctctcgggc ggagacgagc ccccgtcggg cgagcgccgg cccgagcgca
     2461 agctggaaga cctgttaacc gcaggcttga ccgcagctgg cccgcagcgt ataaatgcct
     2521 gcgatgaagg acagtctgcc atccacggag gctggacgtt tgggtaaaca cccccaaacg
     2581 ggaggttatc cagacccgca taatcacggg ttcggcttgt atcacatcgc atctatgaaa
     2641 tacctgttat cttcacctga cccggcccgg atcgggttag ctcaaagcct acttgatgcg
     2701 gcaggcattg agtgggaagt tcgcaacgaa gccgtctccc aaaccgcgat aggcatgccg
     2761 tttgccacgg aactctgggt tctgcgggac gacgactacc aggccgcccg agaccttctc
     2821 aagtccgggg ccgctcgggc ctgattgtaa gcccgcaggc gagccgaaca actgcgtgca
     2881 ggcgacgctg ggttgcgccg tgggttcatt tctgagtcag gggctcagcg cgcctggcgc
     2941 agagtgttag ccacacgagg tgcgaagtgc agaaatatga cccgtcgagc ctgaccgatt
     3001 tgcggcatcg aatcgttgag ttgcgccgtc tgcgccagca gatgaaagat gctaatcggc
     3061 gaccgcgaca gcatcgcctc aatgcttccg agcgggcgca agttctggag aaaacaggtg
     3121 gcaggtgcca catttgcggg ggcgacatta ccggatattg gcacgctgat cacgtcttgg
     3181 cgcatagtgc aggcgggcag catacggttg ataactacct cccggctcac aagacatgca
     3241 ataattaccg gtgggactat cttccacagg agtttgggtt gattcttaag ctcggggtgt
     3301 gggttcgcac gcagatagag aagggaacac caatcggtgc gaacattgaa cagaagttca
     3361 cagcgtacga ggcaaagcgt attggccgat gcaagagccg gaagaggtaa cagagtccaa
     3421 caacagcatg cagcggacgg tgcttcacgc cgccgctgat gctgatcgtt agccagtatc
     3481 agagcgcgca tgaaacctcg acgtctttaa gccatcctcg ccgcggtcct cgcggtagcc
     3541 gtcgttttct gcgtggtcct cctaacctcc tggcagcccc ggcagccgga tttccagaac
     3601 gcgccgaagc tcatttccgc gatgcagggg tattttcgcg tgacttgacc gcacgcgggc
     3661 agcctctgcc accgactgta tcgcggcgcg agttggtcag cggtggatac atcgccgccg
     3721 ccaatcttcg agcctttgac ggcatggacg ttacgatttc gctgacggcc gacgaggcgc
     3781 gaccgcggga gatatttgat tcgagttcgt atgccggacg ggagagtggc tgcgttgctg
     3841 gcaggtggca gtgtccaggg attgcgcgat gagcttgccc tccaaggccg gcaggctttc
     3901 cagcggggcg agggcgacgg ttctctcgca tctatagaaa cttcggcccg ataaccaaag
     3961 atactgcttt cccaggcaac tccgcccccc tctccgaccg cccgaacccc cgccccggaa
     4021 ccagtgaccc gacttgccgg gcaacgcgtc caccccgggc ggtcagccgt tttgtccggc
     4081 gcccgcattc tgcggggagg ctcccagagc tatttttgac tcccggactg gggttgccga
     4141 agggttgggc cggccggaag cggcattgcg tatgggggcg ggattcggta ttagaagctc
     4201 gcaagttcat acggctatga gcaaaccctt tgaaacggaa gcgaccgcgc ccgggattcg
     4261 ggttaatccc ggacagatca gcatcctgat tgcgacgcgg gcccggccgg agattctggc
     4321 gggggttttt gcttcgctga acgcgcacac cacccgcaag gagctggttt cactatggct
     4381 ttacgtggat gaagacgact cgatcacgcg gcaggcgatc gcggcgaagc agttgccgga
     4441 gatggatttg gcggtccatt ggcatgtggg gccccggacg gcttgtttgg gggatacgca
     4501 tgacgcgctg tgggccgcgt cggggcgatg cgccgagatt tacatggttt ccggggacga
     4561 catgcgtttc gatacgccgg gttgggatga agttgcgcgg aaggcgtttg cggcgtatcc
     4621 ggacggggtt ttgctggctt ttccgcatga tccgatgacg gcggataccg ccacctatcc
     4681 gctgttcggc tggggctggc tggcggagtt gcggcggttc ttccccgggt attttccgta
     4741 ttggtatgac gaccggtggg tgagccaggt ggcgcggctg gcggggcggt atgtcaagct
     4801 gccgattctg ctttacccgt tccgcggccg ggggcgcacc cggcggatgc gcaacctgcc
     4861 cttttgggcc cggttcttcc agctcatgct ggaggagcgg aaggaggatg cccgccggtt
     4921 gatccgggcc gtgcacgcga aggacgccgc ggcccgggag gcggcgctgg ggaaggttga
     4981 ggagattgcc gccgcgctga ttcgggagga gggttccttt tcggatcttt acggggtctt
     5041 tcaggaggag cggcattcgg atttgtctcc tgcggagcgg cgggcgtttg atccgcaata
     5101 cgtcgggcgg gaggcgtttg ccattacccg gctggtgagc ctggcgcagc agcagttcca
     5161 ggcggggcgg tttgccgagg ccatggaata tcttgatgcg acccacttat cggacttgcg
     5221 tctccggcag gcgcagcttt tgaaagtgga atgtctccgg aagctgggcc gggatgccga
     5281 ggccggccga ctggagaaag aggctttggc ggcctggccg gaacacagcg cggcccggcg
     5341 gcttttccgg ttcctgggga tggtggccaa cgacgggcgg cggatgctgg ttggcctgac
     5401 ggaaaaaggg cggcgcggca ccgggtagca ggggttggag tcagaggccg gtggggtggt
     5461 ccaggaagac ccagggcacg ttgaaccggc gcgcgaggtg ggcggccagc gccttgactc
     5521 cgaaggtttc ggttgcgtaa tggccgccgt aaaggacgtt cacgcccagt tcctccgcca
     5581 gcgcataggt ccaatgcggc ccttcgccgg tgatgaaggt atccacgcct tccgccgccg
     5641 cctgtttcag gccgtcgccc gcgccgccgc tcacgacgcc gatccggcgg cagacggcgg
     5701 ggccggcggg gatgaggcgc ggcgggcggc cggtggcttg ccgcaggcgt tcggccaagt
     5761 ttgcccgcgg gaggcgggtt tgggtttgaa acccgaggtg cagtccgtgg tcgaagtaga
     5821 aggggcgcag ctttcggaag cccagcgccg cgcagaggcg ggcgttgttg ccgaggcggg
     5881 gatgcgcgtc cagcggcagg tgggaactgt aaaccgccag gtcatgctcc agcaggcagc
     5941 gcagcagttg gtagcggggg ccggtccagg gatgcgccgg cgaccaaaaa agcccgtgat
     6001 gcaccagcag caagtccgcc cgggcggcga ccgccagccg ggctgtggcc aggctcgcgt
     6061 ccaccgccgc ggcgatccgg gtgatggtgc cgcggttctc gacctggagg ccgttggcgg
     6121 cgcgttcgta atcctgcacc cgggccgatt gcagcacccg gtcgcagtgg cggacaatgg
     6181 cggctaaaga ggcttttgac atgcggcgat ggaaacagaa ccgggcgcgg ctgcaaaggg
     6241 ggaagtgggg gcgcgcgggg agcgaggcgc aggcgtccgg cggggcgctt ttctgggccc
     6301 agcacccagc gcccgccaaa ttatccgtcc ctggtattga attctcccgg ccgcgcccta
     6361 tagtgcgcgg gaaaaatcca atgccaccca aaactgaacc gcccactccc gcggggccgc
     6421 cgggccggag gccgtagttt tccgccgccc cgcccgtact agcctgcgca tgtctgaact
     6481 cgtttccaaa gccgaactgc tgcggtcgct ggggcggctg gtgcgcgggt tgtcggcttt
     6541 gttctggggg atgcccctgg cgttgattgt atgcgtgcat acggccaagg cggattcgct
     6601 gcaaccgttt ggcgtggtgc cgccactggc ggcgacggcg ttgctggttt ttggcgtctg
     6661 gcaattgggt gatttccaga agcaggagcg cgtctggggg gccgcgctcg ggcgcgtgcg
     6721 cgtcttcagc ctgatcaact tcgggctgag cccatttctt tattggtgga ataaaatccc
     6781 ggccaacccg ttctttctgg tcatggtcat gctcatggcc ctgtcggcgc tgctgtttct
     6841 ggccagcctg aacctggcgg cgcggcgcct gagcgccatg ctgccggacg aggcgctgcg
     6901 cctggagacc cggcagttca ccacgttcaa tctcaacctg ctcctggtgg cgtttctcct
     6961 cgcgctgctg tatatcgggc tgagcgtgtt ttccaccctg ccgctctggc tgcggatggt
     7021 gggagacgtg ctcgagcgga gcagcctgtg gtatttgatc ctgctgctgc tcctgcccct
     7081 ggccctgacg atggcgctgc tctggaagac caaggaagtc atcttcgaga gcgttttcca
     7141 cgcccatccc tgagcggcgc ggcggcggcg gcggtcggtc agttcagcag ccggtaccag
     7201 ttgtcgcgct ggtgcggttc gtagccgagg tcggcgatga gccggcgcat ctcggcgacg
     7261 gtcatgcgga aggcggtgcc ggcctgcgcg accacgttct cctcgatcat gatgctgccg
     7321 aggtcgttgg cgccgaattt caaggcgacc tggccgatgg ccggcccctg tgtcacccac
     7381 gagctttgca cgctggggaa gttgtcgagg tagatgcgcg ccagcgcctg cgtgcgcagg
     7441 tattcctgcg cgccggccat cggggtgcgc agccgcgtgt gggcggcctg gaaggtccag
     7501 ccaatgaagg cggtgaagcc cccggagcgg tcctgctgcg cgcgcacccg ttcgaggtgc
     7561 tcgatccggt cggcgcgcgt ttcgacgtgg ccgaacatca tcgtcgccga ggaactcaac
     7621 cccagccggt gggcggcgtc catcaccgcc agccagtcgt cggtcatggc cttgcgcggc
     7681 gagacctgcc ggcgcacgcg gtccacgagg atttcgccgc cgccgcccgg cagcgagccc
     7741 aggcccgcgg ctttgaagtc ggccaggatt tgctccagcg gttcgttgaa cagcttttgg
     7801 aagtggacga actcgctggg gctgaacccg tggatgttga tttgggggaa ccggtcccgg
     7861 atgtgcgcga gcaggtccag gtaccactgt ttgcccagcc ggggatggtt gccgccttgc
     7921 agcaggattt gcgttccgcc cagcgcgagg gtttcggcga tcttttgatc cagctcggcg
     7981 cgggtgatga catacgcgtc gggcgcccgc tcggcgcggt agaacgcgca gaacgcgcat
     8041 ccgacgttgc agatgttggt gtagttgatg ttgcggtcaa tgatgtaggt gacgatctgg
     8101 ttgccgcgtc cgtcgtaggc cgccgcccgc gcgatttggc ggcggcggtc ggccagggcg
     8161 ccgagctccg ccagcggcaa atcataaagc cgccgcgcct cggcgggggt gatccgccgg
     8221 cccgtccaga cgcggtgccg caggtcgtcc agggaggggt cgtgattcat gcgggggccg
     8281 ggtcagccct tgagcagcgc cgcggcgtgc ttgcgggcgg cgtccgtttg gccgccgaac
     8341 atgcgcgcca gctcggtcac gcgggccgcg ggttcgaggc gggtgagggt ggagatggtg
     8401 cggccgccct gcacctgctt ggccgccacg taatgggccg cggccggggc ggcgacctgg
     8461 ggcaggtggg tgatgcacag gacctgccgc tggcgggcga tctgccgcat cttctcgccg
     8521 acggcgttgg ccgtttcgcc gccgatgttg gcgtccactt cgtcgaagac caggacgggg
     8581 atctggtcgg cggcggccag aacggttttc agggcgagca tgacgcgggc cagttcgccc
     8641 gaggaggcaa tggcgcgcag ggggcgcgcc ggctcgcccg ggttgggggc gaacaggaat
     8701 tcgagggtgt cgaagccctg tgacggggtg cggccgggtt ggcgcgcgaa ggcggcttcg
     8761 tcgagggagg tgaaggcgat gtccaggcgg ctttggcgga agcccaggtc ggcgagttgc
     8821 cggccggcgg cctgggtgag cggggggatg actttgcggc gcgcggcgga gagggcctgg
     8881 ccggcgcgcc acagcccggc ggtgagctgc tggagcgcgg tgttgaggcg gtccagctcg
     8941 gccgtgcgct gttccagggt ctggagcttg cgccgggcct cggcgccgaa ggcgatcacc
     9001 tcggccagcg ttgcgccgta tttgcgtttc agggagtgga gcaggttgag gcgttcttcc
     9061 agcggcggca gggcggcggg gtcaatgtcg gccttgccgg catagcggga cagctccgcc
     9121 tgcaggtcgc gcaagcccgc cgcggtttgc tcgtgcaggg ccagcagcgg cgcggccgcg
     9181 gcgtccaggc gctgcaattc ctgcaacgtc cgtcccagca ggccggcctg ggtcagcagg
     9241 caggtttcgt tttcgctcag caaatccagc gcggtctggc tgagctgcag caggcgggcg
     9301 gcgttgctgg cccggcgata gtcctgctcg acttgcgcct cctcttcggg gtgcaggcgg
     9361 gcggcggcaa tttcgtcggc ctggaagcgg agcaggtcca actgccgggc atgcgtctgt
     9421 tcgtccacaa tcagcgcggc cttctcggtt tcgagcgcgg cgcgccggcg gacgagttcg
     9481 gcaaaggccg cgcgcggttg ttccaggccg ccaaaggcgt cgaggatggc gagttggcgc
     9541 gcggggtgga ggagggactg gtgttcatgc gggccgtgga tgtccacaag ccattcgccc
     9601 agggtggcga gggtggccag ggtggtgggc gagccgttga tgaactggcg gttggcgccg
     9661 gcggcggtga aagtgcgctt gaggaggagc tggtgatcgg cgcacggctc cagcccgttt
     9721 tccgccagga aagccggcag cggggcgggc aggcgggcga cgtcgaagac ggcctcgacc
     9781 gtgcagctct cgcagccgcc gcggatgagg gtgcggtcgg cgcgttcgcc cagggcgagg
     9841 ttcagggccc cgatgaggat ggacttgccg gcgccggttt ccccggtgat gacgttgtag
     9901 cccggctgca gttccagcgt caggtcggcc accagcgcca ggttcttgat gcgcagcgtt
     9961 gtcagcatgt ctggttgcag gttgaatttg gcgtttaccc cgcccggggg caaaggaaaa
    10021 gtggggcgcg gcgcctccgc ttgcgccggg gaaagatgcg cgctggaggc gggcttgacg
    10081 ccggctgttg gacctgcgac gttgcgtcgg cttgacgccg gattccccca tcgccgcggc
    10141 aagcgaatta ttcgctcgga tgctggaaac tcccgccgtg cgctccctgg cgcggcgcct
    10201 ggagcaggga ggcgtgttgt cgctggcggg catcagcgcg ggcgcgcagc cgttcctggc
    10261 ggcggtggtg cggcaattat tcccgcgccg ccccctcgtc ttcatcaccg acgggctgcg
    10321 gtcgcaggaa agcgcgcagc aggacctgac aacctggctg gcggcggaaa cggaagccca
    10381 cggcgccgaa gccgcccccg ccgacccgcc ggcgccgcgc tccgcccccc tgttctatcc
    10441 cgcctgggaa gtcctgcccc acgaagcgcg gctgccgcat gtggatgtca tcagcgagcg
    10501 cctggaaacc ctggtcgcgc tcctttcgcc cgcttccccc ggcgtggcga gttcgcccct
    10561 catcgtcacc cgcgtggccg cgctgctgca gcgcaccttc gccgcggcga ccctccgcgc
    10621 cgccacccgg acgctggcgc gcggcgaccg cttggatccg ctcgaggtgg ttgaatggct
    10681 cgaggcgcgg ggctacgagc cggaagcgcg ggtgacgcag aagggcgaac tggccctgcg
    10741 tggcggcatc ctggatgtgt atcccctcac cagcccctgg ccggtgcggc tggagttttt
    10801 cggcgacgaa ctggagtccc tgcggcagtt tgatccgctg acccaggtct cgcgcgagga
    10861 gattcaaacc gtcgcgctgg cgcccgccgg cgagctgggc ctgctccggc agtcgcggag
    10921 cgaaaccgct tcccccgcgg cgtccagccc gccgccggcg ccgggaatct tgctcgatta
    10981 cctgccgccg gagaccgtgc tgatattgtg cgaaccggaa gcgctggcgg aacgcgccga
    11041 ggaatacgcc gcgcaggttc ccgccgccga cccgcttttc cttccctggg ccgggttcct
    11101 ggagcaggcg cgggcgcgcg ggctgaccct gctggaagtg cgcgaagccg acttcccccg
    11161 ttccccggcg cctgatcccg ccgcgccgcc ggtcgcgctg cgttgcggga gtctcgaccc
    11221 ctttcggccg ctggtggacc gggcgcccga gccggcggtt gccgaaaccc agcggcgcga
    11281 gtttttcgcc cagcttcacc gttggctgcg ccagggctgg gcggtgcatg tgttttgcaa
    11341 taacaccggc gagcgccagc gcttcggcga gctttgggag gaatatggcc tggccgcgga
    11401 cggcgtcccg gcctgccccg cctccgccgg ggcggccgcg ccggagcctg ccggcgggcc
    11461 ggctcccggc ttcaagctgc gcgcgcattt aggctcgctc gcccgcggtt ttttgtgcga
    11521 cgacgcccgg ctggcggtgg tcacggacgc cgagattttc ggccgctaca aggtgcagcg
    11581 gccgcgccgg ctcaagtctc cccacgccca ggcggcccgg tcggcgctgg acattgattt
    11641 caccgagctg gaggagggcg actacgtggt tcacgtccag cacggcattg gccgctacct
    11701 cggtttgcaa gttccgcctc cgggcgccgg cacccggccg atcaacggcc ccggcgtggc
    11761 cgcgcgcgcc cgcccggaat gcctggttct tgaatatgcg ccgaacgagc ccggccagca
    11821 gccgccccgg ctgttcgtgc cggtcaccga agcgcatctg gtcagcaaat acgtcggcgc
    11881 gggcaaagcg cgcccgccgc tcaacacgct gggcggcacg cgttgggccc acgccaaggc
    11941 ccgggctgaa caggccgtgc gggatgtcgc cagcgacctg ctcgccatcc aggccgcccg
    12001 cgagtccctg cccggccacg ccttcgcgcg cgacgttccc tggcagcgcg agttcgagag
    12061 cgccttcctc ttcgaggaaa cgcccgacca ggcgcgcgcc atcgccgaaa ccaaagccga
    12121 cctggagcgc cccaagccca tggaccggct catctgcggc gatgtcggct tcggcaaaac
    12181 cgaagtcggc atccgcgccg cgttcaaggc ggtcatgggc ggcaagcagg tcgccgtgct
    12241 ggtgcccacc accgtgctgg cgcagcagca cttcaacacc ttccgcgagc gcatggccga
    12301 ttatcccatc cgccttgaac tgctctcccg cttccgctcc cgccgcgccc agcagcaggc
    12361 cgtccgcggc ctcgccaccg gcgcggtgga catcgtcatc ggcacccacc ggctgctgca
    12421 gaaagacatc cgcttcaaag acctcgggct ggtggtcatt gacgaggagc agcgcttcgg
    12481 cgtgatgcac aaggagaagt tcaaaatgct ccgccacctg gtggacgtgc tcaccctcag
    12541 cgccacgccc atcccccgca cgctttacct ggcactcacc ggcgcgcgcg acctgagcac
    12601 catccagacg cccccgcacg accgcctgcc cgtcgaaacc atcgttgccc cctacgacga
    12661 gcgcctcatc cgcgaggcca tccagcgcga gttgaatcgc ggcggccagg tattcttcct
    12721 ccacaaccgc gtgctgacca tcaacaccat ggccgcccgg ctccaggccc tgctgcccca
    12781 ggcccgcatc gtcgtcggcc acggccagtt gcgcagcgac gacctggagg aagtcatggc
    12841 ccggttcgtc aacggcgagg ccgacgtgct gctttccacc accatcatcg agagcggcct
    12901 ggatattccc aacgccaaca ccattatcat tgaccgcgcc gaccgcttcg ggctcagcga
    12961 cctttaccaa ctgcgcggcc gcgtggggcg ctacaagcac caggcctacg cctacctgct
    13021 gctgccgcgc cacggcggcc tcctggccga cgtgcgcaaa cgcctcggcg ccatcaagca
    13081 atactcgacc cttggcagcg gcttcaaaat cgccctgcgc gacctggaaa tccgcggcgc
    13141 cggcaatctc ctgggcgccg aacaaagcgg ccacatcacc gcagtcggct tcgagttgta
    13201 ctgccagttg ctcaagcaga gcatcgccag cctcaaaggc caaaaagccc cgccccgcct
    13261 cgaggtggcc gtgcggctgg actttctcgc cctcggcccg gcggacgaaa ccgccccgcc
    13321 cgcccgtgcg tccgcgcaac ctctttccgg aaatcccgcg ccctccgccg cccacttcgc
    13381 ctgcatcccc ttccgctaca tcgccgactc gcgccagcgc attgaaatct atcgcaagct
    13441 cgcccaggtg accgacccgg ccgggttgac ccgcctggcg caggaactgc gcgaccggtt
    13501 cggcccggtg ccgcctcccc tggcgctgct gctgcagttg gccgaactca aagtgctggc
    13561 gcgcgacctg ggcatctccg ccatcgaggt ccaggacgac aagctcatgc tcacccggaa
    13621 tgcggattat atcatgagcg gcagcaaatt cccgcggttg acgcgcaaac aacccagcgc
    13681 ccggctaaag gagatcaaga aatttctgct gaccctggcg gccaccgcct ccccggcccc
    13741 gcttccgggg gcgtgaacat tcacgaaccg tcccgcctga ataaaggggc acgtccaagg
    13801 tgcgtggaga gtggcggcag gaggagtatt gccgggacgg gcggatggtt ggggccggtt
    13861 tgggtgctgg aacaggggta gatgggctgg attctcggct gttttggtgg acagccgtgc
    13921 ggagcccacg catagtaagg gaaagctcga taaaactatg tttagtgccc gccaactttt
    13981 cgccctgcct cgtatgattg ggagggggct tgccggtttg gggctgctgc tgctggtttc
    14041 agcggcgttg ggggggggag agattccgcc gccggtcatt tcggccgcca gcaaccttga
    14101 ggcgcagcat atctatgtct gcatgctgta ttcgccggat cggtccggag ttgccgggct
    14161 gacaaactat ttgaaacaga ttggagcccg cggctcgggg gctttgtgga cttataccca
    14221 cccgttgctg aaccagatga cggtgattca tgtggtcacg gattttgagg ggatcaagcg
    14281 ggcgctttat accgagggcg caaatgtgct gattctcggg cattccaatt ttggtctggg
    14341 cggagtgttt gccacggcga aggaactgtc cgcgcaacag attaagacgc tgtattatat
    14401 ggacgatgat cggctgatgg tctggtcgtc accctgggtg tcggtgaatg tgccgcggat
    14461 tcgctggcac cagagctttc cgaattggtg gccggacttt caggacggcg ccagcgccat
    14521 tatgccgctg gattttggtg atccccgcgg cgcgcctcct tttaattatt atctaaccta
    14581 tcaactgccc ggggacccca tccattacaa gattgagtct gtggccaata gcgccctgga
    14641 gcgttttccc agcagcggga aaccggcctg gtattcgccg gatggcagcc tgccggaccc
    14701 ggcgaacccc gcgcatcgcc agtattttct gacggccacc aatacggcgt ttgagacggt
    14761 cggaatctgg gtgggcacga atttccagag cggctattat ggccaggatt acctgtgtgt
    14821 gccggggggc acggggcttt gtcaggcggg gtggttcttt tcgattccgg cggccggcaa
    14881 ttataccctc tcggcaacct ggcccggcgg gccgctcaac accacggggg cgtcctatat
    14941 tgtggcgcat gcgggcggga cgacgacagt gaagaagaac cagaagctga atggcgggaa
    15001 atggatcaag ctgggggttt ttgcttttgc cgccggcgaa tatgccgtgg ttttgacgga
    15061 caaagccagc agccagtccc tggtcattgc cgatgcgatc cggattacct ccgtcgccaa
    15121 ccccggggcg tttgatcaag tggtggataa cggcccctgc ccgccgacgc actatggcaa
    15181 gcggacggtt cttttccgcc gcggcctgga tattgatccg gatcaaatga gctatcgccg
    15241 gctgttttat gaaggctgca attccggatt ttattatctc gataccttcc gccggggggt
    15301 ggttttttat accctgcacg actccgacgg gagcggcgcg gccacctacc tgcggtcctg
    15361 cctcgagggc cgaagcgatg aagaaacatg ggcggcactg cagcgcatcc aggcgatcta
    15421 tgactattat gatttcacca aaccgccctc cgaacagggc gcggcggggg cggctgccgt
    15481 tgcggcgccg ccggcttccg ccggcagtca aaagcaaagg gttgcggagt tgagccagtc
    15541 atcggccgcc caggcttttg ccggactgca agaggaggag tttatcagcg atgaaggctc
    15601 cgcggtgcag atggtggctg gcgcgtttgg ccgaaggcgg gccgaaggga ttgccttggc
    15661 gctgaagcag ttggctgttc ccgggacggg aggcgccgcg gcgcgggcgc cgaggggtac
    15721 gcgcggcttc gtcgcagccc gaagaatcct ggccgctttc ccggacgaat ctgtgccccg
    15781 cctgctggag ttgtacgacc ggggcaacgc gatcacacgc ggcaacgtgg tgcgggcggc
    15841 gggagggatg ccgggggatg cccgggtgcg cgattttttg cgcgccgcgc tcgaggataa
    15901 gaacgtgtgt gaggcggcaa gcccggagga actgggagaa ccgctgcgcg tatgcgatgt
    15961 cgcctataac cagttggtgg tcggtttggg cgtgaaaggg gtgctccgca cgattggccc
    16021 catccattcg ctcgcagccc gggagtacca catcggcgtg ctcaaaacca gactgtaggc
    16081 ggattgtccc tcgggcgcgc tcccgcccgc cggcctgaac gggtcactcc gaaaacgtca
    16141 gcacgcagac cttctgttct gcggactgcg gacaaacgcc ccccgcaggc ctgcggcccg
    16201 tgcgccgaca gactggcttt tcgcgtgaag cgggaaagtt cgggctagta atccaggccg
    16261 cgcacacacc ggaagccgat tgtattcaat gcggtctttg gtgcgcgatt gtcgcgggcc
    16321 gagcacttct gggcgggtgc gagggcttgc caggaaccac ttcgcacaac gcgcgcagtg
    16381 ccggtattgg gtccctgcgg gttgtcaacc gggttgttga tgatggtgta gtaagagcgg
    16441 ccgtaccagt cccagcacca ttgccccaca ttgcccgcca tatcgtacag gccatacgcg
    16501 ttacgcccgg atttcagatc gcccaccggg ctggtgtaag gttcacttcc agtgctatac
    16561 gaactgtgga acccgtcggg gccctcgtcg tagatgtaag aggcggtatt gccgaagtaa
    16621 tttgcccggg tcttggagat tttgtcggtg gcccagggga aaaggcgtcc gttggtcccg
    16681 cctcgcgcag cccgttccca ctcggcttcg gttggcagtc ggtagccatt cgcggcccag
    16741 ttaacgtggc cattccccag gttatatact ccggtgcgat aaacatccgc agcgttcagg
    16801 ctcgtactga cgtaatagca aggagtcaag ccgtcctttt ccgagcgcgc attgcaccat
    16861 ttcacgacat cataccagtc caccttttgc acggggtggg tggcagcctt gccggctccg
    16921 gcatggctga aactgtagcc atggcttatc gcccattgat agacattggc ccaaagcgtg
    16981 tagctaacca agttcgagtc catgaggaac gcattgacaa aaaccgagtg ttcgagcgca
    17041 acgccagcgg ttgacccggg cacccccatc agaaagactc cttccgggat ggctaccatg
    17101 cccgccgctg gcgcgggggg tggcggcggt tcgttagtgg gggcaatatt tacgacctgg
    17161 taataccgtt gcggttcgga agcggcactg acatcaaaga accaatacgg actttgtgtt
    17221 accaccaggt ttgtcagcac cgcccagtgg tttcccgtca gcttgtttgt atattgaatc
    17281 tggttggtgt agcccacttt gctttcgatg gtgagccacg gcacacggga gatgttccgg
    17341 atgatgggct gttccgccgc cagcgcggac gtgccaactg tcagggccag ggcgtacagt
    17401 ccgattttta ctcttgggaa ggggtttttc atgcttgcaa tcctgttcgt tatttaatgc
    17461 gaagttttat gatgtacaac ggtttgagtt gttttaatcc tttttctacc accaggggcg
    17521 ctggatagca aggaggcatt cagaaggaag cccaagcgca aagtgtttaa gtcggcgggg
    17581 atgtctgcgc tggtgcggtg gggttttgct cctggctgag gagattcgct gccggggaca
    17641 aattgaaact tgcctccggg gcgagttctg cgcaggttgg ccgggcatga tgaagcaata
    17701 tgcagtcggg tttttgacgc tgatggccgg gttggcgccg atggtcgggc gcgggcaggt
    17761 cggggaaccg gctccgccgc tgatgatcaa ggaatggatt cagggtccgc cagtggaagt
    17821 gaagtccggc acaaatctgt atgtgattca gattttcact accaccagcc tcaccaacca
    17881 gcaaagcctt acgaatttga acgcgttgca gacccggttc aaaggccagg gagtgctggt
    17941 agttggcgtg agcgatgagc cggtggagaa gatccgggag tttatggccc acgacgggaa
    18001 ggtgatcaag catgccatcg cggcggatga ccggcgaaag accgcgctca gctatatgcg
    18061 ggccatccgg cagcggggcg tgccccacgt gttcgtggtg ggcaaggacg gccagatgct
    18121 ttggcacggc catccgcagg acggggtgga ggaggtgctg gagcaagtgg tggcgggccg
    18181 ttacgacccg gcgccggcgg cccgtctgga tgtcgcgcat atccagattg accagtatct
    18241 cacgctggcg cgccagaatg ataaacgggt gccggctgcg ggccggcaat ttttaatcct
    18301 ctggaccaat gacgtggcgt cgttgtgtga tttgacgctg gccatcacgg ccgaccgcgc
    18361 gccccggaaa cgcgatctgg aactggcggc cgaagcgttg gcgcgcgcgg aggaactggt
    18421 ggcgaccaat tctgcgcggg tagccgaggc gcaggcgagc tacctttttg ccactggcaa
    18481 aaaggccgag ggattggccc gggccaaaga ggcggtagct ttcgcccagg gcccccggga
    18541 aaaggcgcgt gcggaagcct ttgtgcatca cctggagacg cgccgggcgg acgacgtccg
    18601 gaaacaagtg gatcaatatc ttttgctggc gcggcggaat gatccagaag ccgcggcggc
    18661 gggccagcga ttgctggtgg gattgaccaa tgacgtcgcg ccgttatgct acttggcggt
    18721 tcaaattgtc agcgatccgc ggattacccg gcgggatctg ccactggcca acgacgcgtt
    18781 gaaccaggcg gagaagctgg cgcccacgaa ttcgacgcgc ctggcggtga gtcgggctat
    18841 tgttcttttc gagaccggca aagaacagga cggtttggcg cgggccaggg aagccctggc
    18901 gactgctcag ggggaaaaag ataaagccct ggccgagaaa tgcctccgca ccatgacggc
    18961 cgcgttcgaa tcggcccgga caaaccaggc caagacaaat ctgcccccaa gcaagtagcc
    19021 aagcccccaa gtgaggttgc agcagcgttc actcgccgcg cctctcgccc tgcgtgccgg
    19081 tgcatctcaa agcgatgaca tctttcaacc ccgagatgcg gaaaacgagc ggccagggtt
    19141 gtccccgcat tttggccgag cacccgcttt gttgtcggtg gaggtatgcc tgccgacgga
    19201 gaccagtccg tgatccggtc gttcgacgac tccgaattgg ctgaggcaat gggggcggat
    19261 gctgcgcccc attcgttgtc ggttttcgcg cccggaattg acaatgccat cactggatgc
    19321 taaaccgata gccagtcccg tccgaggtgc ggtcgccttg gcccggacca gggcgcaaaa
    19381 agccgcctta aaaaaagccc caaaaaaatg gggataagag ttgacgttcg gtcagcggta
    19441 ttataaaagg gaacaaatga aacgcccttg gcggggcgtt tgggaaggaa caaataacga
    19501 acagaatcga aaataacgac agagaaaaaa agtgagaaaa gcggttgacg cctgccgggt
    19561 gctggtgtag aactgcttga gtgagctacc cgagtggggc gcgcattaga tagaacaatg
    19621 cacaactgaa caaacagaac cacataaaaa ccgagacata tgaaaagaaa actactagca
    19681 gcagtaatcg cattggcatc ctgcgcggga acacaaatgt tcgcgcagaa tggtggtgtc
    19741 aatgtcaaag agggcgtgat cacattcgcg ctgactgaaa cctaccaaac cagcgnnnnn
    19801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaatgt caaagagggc gtgatcacat
    19921 tcgcgctgac tgaaacctac caaaccagcg ttagcacgag caccaaggcc aaccagggtt
    19981 cctggagcca gcagccgggc tactacaaga cggcgcaacg gaaagttacg gagcgcacta
    20041 ttctggactg catcggacaa gtgttgcacg gcagcccgaa ctactacagt cggacgggga
    20101 ccagctccaa gccggcagcc cgcctgatcc tgacgcaggg agagttgggt ggcttcttta
    20161 atatagtgcc tgagctcgcc gatgcggaca ccgagttttc ggctgaagga tacaacagcg
    20221 ggtacgctga ggacgccgga ctcgccagca cgattgaggg caccttcgtt cgcctggcga
    20281 cgggtcggca tttcctggcc gttcccgaag gctatgaaac cgaaggcaac tggcctccgg
    20341 gacaccacca gccgtggggg cagattttcg taaaggacgc gcagcggaat ctttgcgaca
    20401 acgtgacctt cttctttgcc attaccgtgc aggagtgcta tgactgcttc tatctgaaca
    20461 gtttcgtgac cgacaccaag tttgtgctca agaaaggcag tgagcaacag gaaggcccgc
    20521 cttgctgcag cacctacatt gaatccacgc tcaccggcag tggcatcgat cgctactaca
    20581 tgagcctgtc ctttgataac accttcaaca atccgtatct taatgacgat aatgaagctt
    20641 ggatcggcgt gtcggatggc ccgtacccgg gagtgcgggg gtacctgaag gaccaactga
    20701 cgggtgacgg aacgactccg gatcagcttc cgtatgttga tcggatcatc tccaatcagg
    20761 gaacgccggc ttggtatgct atgcggttca cgctgaacgg catccttacc tacaagtgga
    20821 agcttgcgaa gtttaatgcc aatgatctgg tgttggattt catccagaac ggtgccgccg
    20881 ccacctatgt ggccaatggt tacagcttcc atgcgctgta ttgcggcctg ttgaccggtt
    20941 ccatgaaaat caatgaaacg ctggtcaaca gcggcaaagc attttgctgt gacaatattt
    21001 ggtgggatga tagctggtat ggggttggct ggaatagctg gcagtatccg tggagtgatt
    21061 actgggaatc gccggttaat gtgcctgctt cgctgagctt gcacgtaggc gtgaacgagt
    21121 tctaccaacc gcgctggcaa tggccgcaga gcagcccgga accgaacccg gcgctccctg
    21181 acgagaccgt ggaaaacctc cgtggcgcgg gtgagaccta tatcccgggc acccgctggt
    21241 atttccttga aggcggatgg ccccaggaat aacgcaggtc agatattagt tttctgacgc
    21301 ttaatcgtta aattatcaac gcaacccaag gcggaggccg gaaggcgccc cttgggttgc
    21361 tttccttata gcagggacgc ggagatatta tgcgaccgaa gatagtgata ttgattctgg
    21421 tggtggcggt cggcttggtg gcactggcgg cggtgtttaa ggggattata aaccggccga
    21481 aaaccccgga gcctggcgat gcagcagcgg tgccgacggc gacgaactca tccacaggaa
    21541 cggcgctggc gccaactcca ggatcgagcc ctgagagcaa aggagaatcg ccatctggcg
    21601 atgaaatcga gcgggaattg caggagatca ccgatctgag tcttgccgga agcggtaatc
    21661 ctcatacggc aggtttattg gctggcaagc tgacgcatcg ggaaaaagag gttcgcgcgg
    21721 ctgccctgga cgctttgatt cacttgaatg acaccaacat tgttcccacg ctggagcaaa
    21781 ccgtgggagt cattgaagat ccgcgggaga aagtggcatt gctagatgcg attgcttatc
    21841 tgaagcttcc tgaagatgcg gagcctccac cagtggtgtc taaagatgct cctgaagagt
    21901 ccatcccgcc gtccagtgtc gttcggtcca agggggaatc gggccaggac aagcaaagaa
    21961 tgtcaactcc gaaatcaccg cgacgagttc caggcaaaac tcggcgtagt gcgattcctc
    22021 cggcagcggg agcccaacaa ctcgcgcctc cggctcccgc cccggatgcc gctccgccgc
    22081 ctccgcaata gccaggagca atctcgaatc aagagcggct tccaatccag ccttgggcgt
    22141 tcagtctgtg ataaagtcac aggcaaaaag tgcctcccgg gcggaagaaa actgaccagg
    22201 gcacgtctcc cgcgcagttt atgaacgaaa cccacggttc cactaattca gcggtgcccg
    22261 cagcttccgt gacgccggca tgggtgtact gggtgcgggg actgctgggg gtggcccttg
    22321 taggggcggg ttacctggcc tgggtttcca ttcataacgg cccggtagcc ggttgcggcc
    22381 cgacctccgg gtgcggccag gtgctgcaaa gcccctgggc ctattggctc gatattcctg
    22441 tcagcgtgcc tgcgctgctg gtttacctgg cgctgctggg gaccacagtt ctgttgcaga
    22501 aacgcccggc gccggacgag gagcgcgggg catgggcggc aatcattgca ttggcggtca
    22561 ttgtggctgg agccgcgctc tggttcgtcg gcctgcagat tttcgtcatc aaggcctact
    22621 gcaagttttg catgattgcc catgcctgcg gccttgccgc cgccttgctt tgtctccggc
    22681 atattcccct ggcgcgcgac cccgccacgc ccatgtgggc cacgggctcg gggctgcggg
    22741 gcgttccacg cgcgggagtg gcttcggtga cggcgattgg cctggcgggc gtcatggcgc
    22801 tggcaggggg gcaactgctg gtgccccgga agctgaacgt ggtgagggac ttgcggccgg
    22861 caggccgccc ggttgccggg caaacaaacg cgatcaacgg cacccccggc gaggggaatt
    22921 ccgcggcgcc cacgcccgcg tccccccagc cccacctcgt cgcgccccgg cagctttcgc
    22981 tttatagcaa cgccttcctg ctccagctcg atacggtgcc gatgatcgga tcgccggacg
    23041 ccacgaacgt aattgtttat gttttcgact atacctgttc gcattgccgg gcgctgcatc
    23101 ccattttgat cagggctcag cgccggttcg gcccgcaact gggaattgtc agtctgccca
    23161 tgccgatttc aaccaactgc aatccctttc tgccgccgca gtcgcactcg attgcgaacg
    23221 cctgtgagta tgcgcggttg ggcctggccg tctggcgggc caagcccgcg gtccacggcc
    23281 agttcgagga gtggatgttc gcgggcgccc gcccggcgcc ggtggcgcag gcaaaagaat
    23341 acgccgcgca gttggtcggc ccggacgtcc tggaagccgc gcttaaggat ccctggattg
    23401 agcagcaact cctgcttgat tgccggctgc atcacatcaa ctggatggtc agcgagcagc
    23461 cggtcatgcc ccagttgatt atcggcgatg ccatcagcac ggggccgctg aattcagtcg
    23521 agcacctggt gctgctgctg aaccactacc tgggtctcac cgcgccacca ccgccggtat
    23581 ccgggggaca aggcgaggcc agtttctgac gctgatgata cgttttatga tgcgaagagt
    23641 gaaaacctgt tccgctctgc tcactgcggc cctgctcctc tccggctccg tccgggcggc
    23701 ttcgcccgcc ccgaccaacg cggcgcccgc atccccggtt tccgccaccg ctaccaataa
    23761 agtggtagcg cgcggcaagg gctgggaaat cacgcgcggc gaactgggca agggagtcac
    23821 tgcggcgatg gcgcaggtgg cggctcgagg ccgccaggtc acaaccgcgc aggttcccga
    23881 ggtggagcgg caggtattgg cacagctcat caatcttcgt cttgtgctgg cccgggcgac
    23941 ccgggccgac cgggaagccg gcaaagccgc cgcaaataag cgttatgcga ccgccagggc
    24001 aaacgctgcc tcggaggagg cgttcaagtt gcagttgaaa ttcctggtga cgaccccgga
    24061 ggaattgctg gccaaatgga ctgatgcgct aacctccgag gcggtgatga agcgggaact
    24121 caagatcgcc atttcagacg cggaggccag gaagtactat aacgagaatc cgaagcaatt
    24181 tgaccttccg gagcaggtcc gcgccagcca tatcctgatt gccacgagcg atccccggac
    24241 gggtgccggg ctgtcggacg aggagaaagc cgggaagcgc aagaaagcgg aggcggtgtt
    24301 gcagcaggcc cgggccggcg aggatttcgg caagctcgcc ttggcctttt cgaacgacgc
    24361 cgggtcccgc gcccggggcg gcgaggttcg gtttgcccgc ggcgagatgc cggcggagat
    24421 agaggccgtg gcattttccc tgaaaacaaa ccagattagc gacctcatca ccaccaccaa
    24481 cggctaccac attatcaagc tgatccaaaa gattcccgcc cgcaaactca agtatgccga
    24541 cgcagccgcc gacattaaga acggcctgac gcaggccgca atcgaggagc aattcccggc
    24601 ctacattgcc cgcctgcggg acgcagcgaa cgtggaaatc ctggaggaac agttgcagcc
    24661 gcaggacccg gcgcgcaccg ggctgtcgct gcccggcccc ggcgcgccgc cgaagcctgc
    24721 gggcgtaaag taggcgcttt gccggcgatc atccgcgccc gggcccggcg cgcgccatgc
    24781 cggcgggacg cagggccagc ggccgggttc ttatcagggt tgatttgcgc cggttgttgg
    24841 cggtattgat ttcccattct tatgccattg aaagttgttg tcatcggcgg cgtggctgcc
    24901 ggccctaaag ccgccgcgaa gattatgcgg ctggatccca ccgccgaagt gaccttgttg
    24961 gagaagggac agttcatctc ctttgccggc tgcggcctgc cctattacgt ctcgggagtg
    25021 gtgaaggaac agaaagacct gatgagcacg ccgatcggag tccggcgcga cccggcgttc
    25081 tttcagaagg tgaagaacgt caaagtcctg acggaaacgg aggccctgga gatagaccgc
    25141 gcgcgcaagc gcatccgcgc ccgccgcaat ggcgcggcgg aggatttctg gctggattac
    25201 gacaagctgg tgctggccac cggggcccgc cccgtcatcc cccccgtccc caacgcaacg
    25261 ctggccaaca tcctcaccct gcacagcgtg gcggacgcgg atgccatcaa ggcccggctc
    25321 gcccggaacc acgggcggga cgtggtgatg atcggcggcg gcttgattgg cgtggaaacc
    25381 gtcgaggcgc tggtgcaatg cggctgccgg gtgacggtgg tggaacggct gccgcagatt
    25441 ctgcgcatgc tggattggga aatggcgcgg ctggtggagc agcacatgga agctcacggc
    25501 gtccaggtga tgacccatac cgaggtccag gcgtttgaag ggccggggca ggtgacggga
    25561 gtcgtgacga accgcgggcg gctgcccgcg gacctggtgg tcctgggcat cggcgtgaag
    25621 ccgaatgtgg aattggcccg gcaggccggg ctggagatcg gccccaccgg cgcgctgcgg
    25681 gtggatgccg gactgcgcac ctcggacccg gatatctacg cggcgggcga ttgcgcggaa
    25741 agcaccgacc tcatcacgcg ccagccctgc tacgtgccca tgggctcgac ggccaacaag
    25801 caaggccgcg tggccgccat caacgtctgc ggcggggagg accggttccc gggcgtgctg
    25861 ggcagcacgg tctgccaggt ctttgacttc tgcgtggcgc ggacgggctt gaccgaggcc
    25921 gccgcccggc agagcggcta tgcccccgtc acggtgctgg tgccggatgc ggaccgggcc
    25981 cactacatgc cgacggcccg gccggtgatc ctcaagctgg tggtggacga gcgcacgcgc
    26041 aagctcctgg gcgcccaggc ggtggggccg ggcgcgggcg acaagcgcat ggacgtggcg
    26101 gcgatggcga tcacggcggg aatgacggtg gaccagttgt cgaaggtgga cctctgctac
    26161 gcgccgccgt attcgcccgc cctggacaac cttatcctgg cggccaacgt cgcccgcaac
    26221 aagctggatg gcctgctgca cggcttgacg gccagcgaag tccagcagcg gctcgaagcc
    26281 cgggcggatt tcgtcctgct ggatgtgcgc agcccggcgg agcatcagca ggagcggctg
    26341 cccggctcga cgttcatccc cctggggacg ctgcgggcgc ggctggacga attgccgcgc
    26401 gacaaggaaa tcgtgacctt ctgcaaagcc tcgctacgcg gttatgaagc cgcccgaatc
    26461 ctgcaaggcg cgggattccc gaatgtccgg gtcatggacg gcggcctcgc ggcctggcct
    26521 tttgcaaagc tgtcgggcta agccccgcgc gcccggcggc ggcggggccg gggcggccgg
    26581 ctccgggccc gcggattcag acgcccccgc ttttgtccac cgcctccaga atggcttgaa
    26641 tggcctcgcg ccggatggcg cccagttgat ggttgtcccg cgtgggatgg acggcattgg
    26701 tcagcagcac caggtacatt tttcggctga gttcgatccg caggtaggtg cccgtgaacc
    26761 cgttgtggct caggacccgt ttcgcgcccc gctcaatccc ccaccccaag ccgcgcccgg
    26821 aatccggggc caggttatgc gggcgggcga attccgccaa caccgcgcgc gtgagccact
    26881 ggctttggcc ggcgtccgcg cgcaacagtt cggccgccag tttgcccagg tcttcggcgg
    26941 tcgcgaacag ccccgcgtgc ccggtgaggc cctcgccggc gcgcgcgttc tcgtcgtgaa
    27001 ccaccccatg cgcgaagcgc tcctggtccg gcagtttctc cgtgggggcg atgcgcggcc
    27061 ggagccccgg cgcggggttg cgcagggtat tcgtcattcc cagcggcccg aagaccagtt
    27121 cccgctccag cgctgccagg ggtttgccgc ccgcgcgcgc ggcggcttcg cccgcgagga
    27181 acagccccaa atcgctgtag cgggtgcgcg cgcccggctc gctctccagc ggcgtcgtca
    27241 ggatggcgcg gagcatggcc gcgtagttgg tgtgcgagcg gtagtaaggt ttccaggccg
    27301 tcagcccggc ggtgtgggtc agcaggtgtt cgagggtgac ccgctggcgc caggctttgt
    27361 cctcggcgct gggtgccagc gcaaggaact cggggatgaa ccggctgacg ggatcggtga
    27421 tgcgcacctt gccgcgctga accaattgca tgaaaaccgc ggtggtgccg gcaaccttgg
    27481 tgaccgacgc cagatcgtag agcgtctgcg tcgtgaccgg cgcgcccccg gcgtaatcca
    27541 ggcgcccaaa cgccgccgac cacagaacct tgtccgccgt gcccacgacg accgtgcagc
    27601 ccggaaaggc gcgctccttg attccgcgct gcagtatggc tgcggccggg gaaaagtccg
    27661 gcgtgctggc cgccgggcca tccagggcgc cgaagagcag gccaaggaga cacagcatcc
    27721 ccccggttcg aagcccgccg cgaacggcgg cccggggatg gttcccaggt ttcccgcggg
    27781 ccggggtacg gcggggccag gccgggagga cggaagattc aggagttcgg cagttcataa
    27841 agcactttgc gcccgcagtc tgggcgggtg cccgcggaaa agccagccgc atttatccgt
    27901 cccggcggag gctggatttt ggcggcccaa ccccaccgcc tgccgcccgc cgccgggcgc
    27961 tattccgcct gttgcgcctg tttggcgcgc ttgctttcct cgatgatctt ctgctccgcc
    28021 tcgcgcggca tctcctcgta atggctgaac gactcggaat gcgcgccgcg cccgccggtc
    28081 agcgagcgca gggtgctgga atagcgataa agctccttgg ccggcacatg cgccttgatt
    28141 aattggatgc cgccctccat gtccatgccc tggattttgc cgcggcggct ggagaggtcg
    28201 cccatgacct tgcccatgca atcatccggg atgcggattt cgaccaggtg aatcggctcc
    28261 agcaggcagg gccgcgcctt ttcaaaggat tccttgaacc caaaataccc ggcaatctgg
    28321 aacgcaatgt ccttcgagtc caccgggtgc attttgccgt cgtaaaactc aatcttcacg
    28381 tccaccaccc ggtagccggc caggatgccc tcttcgcacg ccttcatgac gcccttctcg
    28441 acggaaggca cgaatacccg gtccacgttc tgcccggtga gcgtttgcgt gaactcgacg
    28501 ccggtatcgc ggggcttggg ctcgatgcgc atccagacct cggcaaactg gcccgccccg
    28561 ccggtttgct tcttgtggcg gtatttcgag tcgccccgcc ccttgatggt ctcgcggtag
    28621 cgcacgcgcg gctcgatcag ctcgacatgc acgttgtagc gccgccgcag gctctcggcg
    28681 atgacctcca ggtgcagctc gccctgcgcg gacacgatgg tctggcgcag ctccgaatcc
    28741 accacgtatt gaaacgccgg atcctcatgg tgcagcgccg ccagcccgga cgccaccttg
    28801 tcttcctcgc ccttggcgct gctcttgagc gcggcatgga tgctcggctt gggatattcc
    28861 accttcggca gcgaatagag cttcttcccg ccataaaggg tattgcccgt gtgggtgtcc
    28921 ttgagcttga ccaccgcccc gatgtccccg gccacgagcg cgggcacgct ggtgcgcgtc
    28981 ttgccattga gctggtaaat ctggccgatc ttttccgtgc tgcggcggtc ggcgttatag
    29041 acttcgctgc cgaacttcac cgcgccggca tagacgcgga aaaaggaaag ctcgccgaac
    29101 tgcgcctcgc tcatcgtctt gaagatatag agcaccggct cgggatccgt cagcttgacc
    29161 ggcaccgggt tgccgtccgc atccgccgcc tccaccgcgg gccgatccgc cggcgaagcg
    29221 ccatactggg caagaaaatc catgagctgg gcgacgccga cattgttttc cgccgccgtg
    29281 cagaacaggg gggtgaacac ctgcttctgg aacgccgcgt gcaaacccgc ccgcatctcg
    29341 gcctccgaca gcccgccttc ggcaaagaac ttctccatca gcgcgtcgtc ggactcggcc
    29401 acgtattcga ccagttggtt gtgcaactgg ctggccttct ccgccagggc cccggcgggg
    29461 ggcgcctcgg tgtatttgcc gctcttgtcg cccgcatagg tcatcacctt ctggcgcagc
    29521 acgtccagca actggttgaa gcccgggccg gcgttgaccg gcagcgtcag cggcagcacg
    29581 cgctccccga aatgctcgcg cagctgcgcc agcgtcgcgt cgaaatgggt ctcttccttg
    29641 tcaaaagcat tgacgacaat caccttcgga atgccgttcg ccgtcgcgct tttccacacc
    29701 gtgtccgtgc cgaccccggg gccgtggttg gcatgcacca ccaccaccgc ctggtcgctg
    29761 acccgcagcg cgcccagcgc ctcgctgatg aaatccgcgt agcccgggca atccaggaca
    29821 ttgaacttct tgccctgcca ctccaggtgc atgagcgtgg tggagacgga gatcagccgc
    29881 tgctgctcgc tctcatgata gtccgagacc gtggtgccgg cggggatggt tcccatgcgg
    29941 ttaatcacgc cggcacaggc gagcatcgcc tcgctcagca tggttttgcc gcacgaggca
    30001 tgtcccacta tcgccaggtt tcgaaggtca acagcctgat attctttcat aaagtgtcac
    30061 ggtccatttc gccaatccct tttcaagcca atgctcaaag ggacctacaa tgtagagaac
    30121 gacccgccgc ctgcaagccg agaaatcagc cgccgcgaaa aatcttcgcc ggccccgcga
    30181 agaacggttg acaaatcccg ccccttgtgg ctgaatacgc tcgcgcccta ttcgggcggc
    30241 cgtgggtagg atactcaagc ggtcaacgag ggcagactgt aaatctgctg gctaacgcct
    30301 acgaaggttc gaatccttct cctaccacca cctccccagc tccctccccc gcccggcggc
    30361 gccctttcgc ccgaacgcgc gcccgccgcc cgccgccccg cccggcgcgc cgcgcggcgg
    30421 cgttgacaaa ggcgcgcggg ccagccaaaa gaggaagcac aatgcaaatt atccgctacg
    30481 aagatcccca gggcgccgtg cgctatgcgg cccggcagcc cgatggctcc gcccgccaaa
    30541 tcgccggcga catcctgggc gcctacgaag tcacccccac cccggcgcgg gtgaagaaac
    30601 tgctcgcgcc cgtggcgccg gtggccctct tctgcattgg cctgaactac cggcgccacg
    30661 ccgaggaagg caaggccgcc ctcccggcct tcccggtgct gttcatgaaa tcgcccgccg
    30721 ccgtgcagaa ccccggcgat cccattgaac tgccgcggca cctgcgcagc gacaaggtgg
    30781 acttcgagtg cgagctggcc gtcgtcatcg gccgccgcgc caaaaacgtg tcccgcgcgg
    30841 acgcgctgga ctacgtgctg ggctacacct gcgccaatga cgtcagcgcc cgcgactggc
    30901 agggcccctg gggcggcagc cagtggtgcc ggggcaagac cttcgacacc tttgccccgc
    30961 tggggccatg cctggtgctg aaggacgaaa tccccgaccc cgccgccttg aagctccgga
    31021 cgctcctcaa cggccaggtg atgcaggact ggcgctgcga cgacatgctg ttcgacgtgc
    31081 ccacgctcat ttccttcctg agcggcagca ccacgctgct cccgggcacg gtcatcctca
    31141 ccggaacgcc ccacggcgtc ggctttgcgc gcaagccgcc ggtgtttctc cagcccggcg
    31201 atcgcgtcac catcgagatt gaccgcatcg gcgcgctgac caatcccgtg gtcgaggaag
    31261 gcggagagtt ccccccgccg cgcgcgccgc gccgcgaccc ggcgggctag ggccggtacg
    31321 tgaagatggc gctggcccgc gcggcgcgat ccgtcttcag ccgcacccag tgggcggaat
    31381 aaccggcggg gaaccggtgc ttgagcgtcc ggccgggcgg gaccaccagc tcgcaatacc
    31441 ggctccactc gccgttggcg gcaaagtcca cctcgatcgt aaaggcaacc ggctgcgcgg
    31501 cccggtgcga aagcgccagg gacttggtcc gataccccgc catgagatag ggatcgctgg
    31561 gttcgttggc cgccaccgcc gtatccttcc acggaccgcc cacgccggcg ggcgcgccca
    31621 ggcgccagag atcgtccacc tccccaaacc acaccgccgc cgcgccatcg gcggagggat
    31681 ggcaatgccc gtccggccgc gcgccggccc gcacccccgt cagcaccagc aggccgcgcc
    31741 aggccgcaaa gtcgcggatg cgcttgtggt gcgtggtgat ggggcgcaag cgctgaaacc
    31801 cgcccgcgtc cgcgcgcggc acttcgtaga tcgtgccgtg aacattgaag aggttccgct
    31861 cggtgacaac ctcgcgcaga gcgcgcggcc agccggtggg gaaaggatcg tcatagacgg
    31921 cggccgtccg cggcagccgg tagcgctggc cgccgtgggt gaccaccacc gaggcggcct
    31981 cgacggccag ggccggcccg gcgccgggcg gcgcgcgcag cgccgcttcc ttgtccgcgt
    32041 cgtccagcgg ccgccattcc agccggccgt ccatgaccca gccccgccgc tctttcaccc
    32101 gcgcgccttc gtagaggttc gccgccagaa tcagccggcg cgcatcgccc gcgtcgggct
    32161 tcaccagccc atcaatgacc ggcccgcggt aatccgcgtc cgccagggcg gcgaaccggg
    32221 cggggtccgg ccggcgcggc gggttgccca ggtgaaagta cgcgctggcc cggcgggcgg
    32281 cctggccggc tcgcacccgc acccagccgg ccttgagctc gcggcccagc acgcgaaagg
    32341 cgtagccgtg ggcgggcacg gacacgccgg ccaccggccg ccactggccg cgcccggcgg
    32401 cgtcagcctc cacggtaaag tccaccgcgc tggcgctgtg atgcttcagg tgcagcgttc
    32461 cctcgcggaa cccggccacc aggaacggcg gagaaaccgc gccggcggcg aggtcatcgt
    32521 tgagccaggg cccgccccac ccggcgggcg cgccgagacc ctccaggtcc gcccagcttc
    32581 cgaaccaaag cgccgagtgc gactggcccg ccagttcatt ctgcatgatc gaggcgtcgt
    32641 cgcgcgccat gacgatttgc ccccgccagg cgcaataatc caccggcatg cgcgtgtagg
    32701 tggagagcgc ccgcagcccg ccgtaatggg ccgtcccgaa ggtgggcggg aagtcataaa
    32761 acagcccgtg catgtgggcg agccagcggc cttcggtgac ctcgcggatg cggggccatt
    32821 cggtgtacca gccgtggagg gcatcctggg tatagctgcc cttgggcagg cggaaggtgt
    32881 gccattggcc gccctcccgc agcttgagga tgaccgagcg cttgtcccag cccagcgccc
    32941 acaggcggtc gtcgcccggc gccaccccgc gcaaaccgcc cgggccggca acctcggtga
    33001 acggcgcgcg ctcgatgacc cgccatgcct ggctccaatc gcggccatcc gtctccgcca
    33061 ggcagccggc gggaccgtcg aagcccgggt cgccgtcgta gcgccagccg cgctcgccgt
    33121 tgttcgccac cacgagccgg cccgcgctgg tggccgcccc cttgccgtgg gcgccgggat
    33181 aaggcccgcc gagcaggcca tagatccggt tcgccgccag cgtcttgacc tccacctcat
    33241 acaactcacg ctccatgccg acgaaataca ccttgtcggc gggcgcgacg aggtgccggg
    33301 cgttgcccgt aatacgcagg ggcatgcggg ccaggtccac cgtacgcacc cggccggcct
    33361 cgtcaataaa gtgcggtccg aggataagct gccgggattc ggggtggatc atgcggttgg
    33421 cgtgggtgcc gccgacgctt tccggccgca tctccagctt caggcgcgag tccaccgtgt
    33481 agagtttatc ctggctcccc tcgggggcat ggggcggata ggtgataaac cagagcttgt
    33541 ccgcccaggg cgccaccgcg ccaatgccgc actcgccgta gggattgaaa accgccaggt
    33601 gcgggtaaat gccactgatg caaacgacct tgttttcggg atcaggcggc cagacctggc
    33661 cagccgtttt gggcgccgcc gccggggccg caacccggcc acccaacgcc aacacgctta
    33721 aaccgacgag caggtaagaa agaaggcagc gcacccccaa gtctctagca catccttatc
    33781 cgccgaggca atgaacaata caatgccgcc ccatgctcct gaaaacaccc tcccactccc
    33841 agcccaagca tctccccacc tccgcccacc cgctaaaaaa gccgaaacta agccaaaacc
    33901 ccaccgcttc agcccccaaa gtcaaaactt ttcatctttc gcctatccac atcatttcca
    33961 gctacttcca ccctgccatc gagccgtgta caaggggtgt acaagggatg ctcaccggat
    34021 gctcatcaga ttttaccatg agcacccgat gagcatccct tgtacacccc ttgtacacgg
    34081 ctcgatggga gctatggaag atgctgaaag gacgttggtt gagctaagtt gggaggggtg
    34141 gttgccgggg agcgaaattg ggagaccagg cggagggaga gagggtgtcc aataattgga
    34201 cagtttgagg atgggagcgg ttggtgacgg gacggttatt ggggaggtgg cttgaggttg
    34261 gttcggatgc ttttagcggt cttgtgggtg gcgggcaggg agggagagaa tgaggggtcg
    34321 cgcctttttg tgcttatgcc ggaggaatgg agccgggggc aggtttggct attgaagttc
    34381 ggctccgggt cggttagcct gccgtagtcg tatgacctta acagagaaag tattggcccg
    34441 cgcggcgggc aaggcccggg tggcggccgg ggacaacatt tgggtgcagg cggatgtgct
    34501 gatgacgcat gacgtttgcg ggccggggac gattggcgtg ttccaccgtg aatttggcaa
    34561 gggagcgaag gtgtgggatc ggcaccgggt ggtgatcatt cccgatcact acatattcac
    34621 cgcggattcg aagtcgaatc ggaacgtgga tattctgcgc gagttcgccc gggagcaggg
    34681 gctggtttat ttttacgatg tgattgacga cccgaacggg cattgggtgt ttgacgcgag
    34741 caagggcatg ctcaagcggc agtatgggtc gcgttatgcg ggggtatgcc atacggcgct
    34801 gccgcaaaag ggccacaccc ggccggggga ggtgttgttc ggcaccgact cgcatacctg
    34861 catggcgggg gcgttcaatg agtttgccac gggcatcggc aacacggatg cggggtttgt
    34921 catgggcacg ggcaagctgc tggtcaaagt gccggagacc atgcatttcc ggctggaggg
    34981 caagctgccg cccggggtga tggccaagga catcattctg cattgcattg gcgaaattgg
    35041 ttttgacggg gcgacgtatc gcgcgatgca gtttgacggg ccgggggccg ccagtttgag
    35101 catggatgac cggatgacgg tggccaacat ggcgatcgag gcggggggga agaatgggat
    35161 tttcgagttc gacgcgcaga cgcaagccta cgtggattat cgctgcaaac tcaacgggac
    35221 gaaggcgggt tatgagccgg tcgagcgcga tcgggaggag aagtttgtgt atgagctggt
    35281 gctggatctt tcccggctgg agccgacggt ggcctgtcat cccaatccgg ggcagcgcaa
    35341 gctggcgaag gagctggggc atatccggct ggatcgcgcc tacattggct cctgcaccgg
    35401 cggcaagacg agcgattttc tggaattcgc gcgggtggtg caggggcggc gggtggtgat
    35461 tgacactttc ggcgtgccgg cgacgccgga gatcgtgcac gatttgcaga cggcgcgctg
    35521 gggcgagcag acggtctggg agattcttac ggccgccggc gtgctgatga cggaaaacgc
    35581 cggctgcgcg gcgtgcctgg gcgggccggt ggatacgttt ggccgcatga acgcgccgct
    35641 caagtgcatc agcgccacga accgcaattt ccccggccgc atggggcata aagattcgca
    35701 ggtgttcctg gcgtcgccgg ccaccgtggc ggccagcgcc gtgacgggcc ggattacgga
    35761 tccgcgggag ctgctgcccc gggcttgatg cggcgcgaaa cgctggatac ctggtgcgag
    35821 cggggcatcc tcggcctggt gctggtggtc ctgatctggg ggccgctggc gatgggagcg
    35881 gtgcgtccgt ccgagttcct cgtgatccag ggggcgacga tgggcgtgct gctgctgtgg
    35941 ggggcgcggt tgtggctgag cccgcggccg caggtgctct ggccgccgat ttgctgggtg
    36001 gtggcggctt ttgtggggta tgccctgatc cgctatcatt acgcggaggt tgagtacctg
    36061 gcgcggcagg agctgatccg cctgctgatc tacgcctttc tgttctttgc cctccttaat
    36121 aatttgcacc ggcaggagtc gttgcaggtc atcagcagca cgctgatcac gctggcgatg
    36181 gtcatcgcgg cctatgccgt gtatcagttt ctgacgcgct cggactgggt gtggcggtac
    36241 cgggtcgggt ttgattatgg ggggtcgggg acgtatgtgt cgcgcaatca cctgggcggt
    36301 tttctggaga tgctcctgcc gctggggctg gcgtacacgc tggtgagccg gttcaagccc
    36361 gtgagcaagg ttctgacggg gtatgcctcg ctggtaattc tcgccggcat tgtggccacc
    36421 gtgtcgcgcg ggacttatct ttcgacggcg gtggcgctgg ggttgttttt tggcgtgctg
    36481 ctgtttcagc gttcgcaccg gctgccggcg tttctgttgc tggtggtcgt gctggcggcc
    36541 ggggcgtatt tgctgccccg gaacatttcc gtgcggctgc gtttccagca gtatttgacc
    36601 gagcccggcc cggatccgcg ggcggaggac ggcaagcggt ccgttttctg ggatccggag
    36661 gatgcgcgcg tgattctctg gcaatcggcg ttgcgggtgt gggagcagaa tatctggtgg
    36721 ggcgtggggc cggcgcatta tgacgtgcgg ttcccggcgt atcggccgtt ggcgatgcag
    36781 ctgcatccgt attttgcgca taatgattat ttgcagtttc tggccgagtg ggggctggtt
    36841 ggcaccgggc tgggggcggc ggcgttggtt ttgctgggct ggggggtggt cgggagctgg
    36901 cgctacgtgc gcggcgcgac gagcgatctg gggcatcccc ggaacagcag caagttcgcg
    36961 tttgtgctcg gggcggtgat ggggctggtc gcgattttgg tgcattcggt cgtggacttt
    37021 aatttgcata ttccggccaa tgccatcctg gcggtgacgc tggcggcgtt gctgagcagt
    37081 tgcctccgct tcgcttcgga gcggcattgg cagtcgctga agtggtgggg caaggcgctg
    37141 ggcagcgttc tggtgctggc gggcacggtt tacctggggc agcaggggtg gcggaatgcg
    37201 cgggagtacg cctggctcca gcgcgcggat cgcgcgccgc tgttcaccat ggcccaggtg
    37261 gattgcctga agcgggcttt cgccgccgag ccgatgaatg ccgagacggc gtatgccatc
    37321 ggcgagattt accgcgtgaa atccgcggaa ggcggggacg attatcagga actggcggtt
    37381 caggcgctgg agtggtttaa gcgcagcctc gagttgaatc cgtggaatgc ctacgggcat
    37441 ctgcgctatg gctggtgcct ggactggctt gggcggttcg cggagtcgcc gcccttcttt
    37501 gcgcgcgcgg cccagcttga ccccaatggc ttcgaggtgg cggccaatgc cgggctgcac
    37561 tatgcccagg cggggaacta tgccgccgcc cggacgtggt tcgagcgctc gctgcggctg
    37621 gaaaggaagt ccaacccgat tgccagcagc tacttgccca tcgtgaccag ccggttgctg
    37681 gaggcggcga ctaatgagtt tagcgccggc cttcggtcgg cgtcaccatg agggcggcgc
    37741 ggcccgaaac ctgctggcaa atgccggtcg gggcatattt ttggggcgcc tggccgcggt
    37801 gagaaaaatt gaaaatattc cgttgacaaa gccgggccaa ccgctaatat tagcccgtga
    37861 ttgatgcagc ctagtttgca ggagcaaata tgaaaacgat acaaagcttg atctataaat
    37921 tgctggcggg cggggtggtg acggcgatgg ctttcacatt ggcggcccaa acggctgacc
    37981 aggggatggc caaggtggtt cgtctcaagg gtgcggcccg gtatgtctcg gggagcaagg
    38041 aagggcaact gctgaaggtg ggcgatttga ttccgccggg cacggccatt caaaccgcgg
    38101 aaaaatcccg ggtggatttg gttttgggcg aagggtccgc cccggtggga ggtccgccgg
    38161 cggcgggaga tatgctcacc tacaccccca ccgttgaaca gaatgtggtg caggtctggg
    38221 agaacaccct gttgggcata gacaaattga atattacgcg gaccgccggg gacgaggtga
    38281 cggagacgca gctcgatctg aaggcgggcc acatctttgg catggtgaag aagctgtcgg
    38341 cggcctccaa gtacgaggtc aagattccca atggcgtggc cggcatccgc ggcacggttt
    38401 atgacatcag cgcggagggc gtcatcaagg tgctgtcggg ttcggtggtg ctggcctatg
    38461 tcggcgccga tggccaggtg cagacccagg tggtcatgag cttgcaggag tatgacgcgc
    38521 ccagcaatac gctgaagccg ctggcggaca tcaacaagag cgggatggag aaatgcatgc
    38581 acggctggcc gggtcggccg ccgccgccgc gtccgccgcc gccgccgccg ccaccgccgc
    38641 cgtcgcgctg gaagccgggg cacgggcatc atcacgggca ccactaagag tgccggcggc
    38701 ccggttgcaa ccgggagggc tttaatcaac cagagcagcc ttgtgttgct ctggtttttt
    38761 tgtttttttc tccgaagtga agcggttgcc gatcaagcgt gttccagccc tgattaccct
    38821 gggcgtcatt gttttgatca gcctggtgcg ggggtggcag tttaactttc tggaaagcct
    38881 ggagcggatg acctatgacc tgcgggtgcg ggccgcgctg cggcacgcag cgccgctggc
    38941 caccaacctg gggtttgttt tcattgatga cgccagcatt gcctttgtgc gcacgaacac
    39001 gacgctcggc tataattatg ggctgtactg gccccggcag gtttatgggc gcctggttca
    39061 ggagttggcg gcgcaaggcg cgcgcgcggt ggccctcgat gtgattttcc acgagttgcg
    39121 cccggatcat ccgccggtgc gaatggccga cggcagcttt ccggagtcgg atgaattctt
    39181 tgcgctccag atgcggcggg cgggcaatgt cattctcgcg ctgcccgagg atttgacatt
    39241 gccggcgctg tttcagacca atgcgctggc cctgggcgac atctcgacgc ataaggacca
    39301 tcccgagggc gttttgcgcc gcgcccaggc gtttcgcgtc cggcggcatt ggcactttgc
    39361 ctttcaacag gtggcggcgg acccggagtt tggggtggat ttgcgccggg cgcgggtgga
    39421 aggccgccag gtggttttgc cgcggtccgg cgtggaggat atccgggtgc cgctggacgc
    39481 ggagggctgt tttgatttgg cggattttgc cggcgataaa ttgccgcccg gggtcgcccg
    39541 cagggccaag ccgttcacgg aggagcgcat ctggcacatg ggcatcgtgc tggccgcggt
    39601 ggaactgggg ttggacctgg ccggggccga ggtgcgtctc gaccaggggc ggataacttt
    39661 gcagggcgcg cacggtgttc gccgggacat tccggtggat gccgacgggt atttctacat
    39721 caactgggcc ttgccgccca accatccgca actggcgcag gagtccattc aagcgctcct
    39781 gcagcaggac ggcctgcgct tgcagcagcg gacgaacgag ttgcacaacc actggcgcgg
    39841 caagctggtg gtggtggggt ccagtgccac gggcaacgat ttgaccgacc gcggcgccac
    39901 gccgctcaag gcggacaccc tgctggcgag ccagcattgg aatgtggcca actcccttct
    39961 gaccgggcga ttcgtccggc gggcgccgtt gggggtggag tgggcgctgg tggccttgct
    40021 ggccgtggtg gcggcggtgg tgacctggaa aatgcgcgtg ctcgtggcgt cggcgctgct
    40081 ggttttgctg ggagcggctt atgttggttt gggctttgtt ctgtacgcgc ggacctgcta
    40141 ctggctgccc ctggtcctgc cggtggcggg ttctctgctg ctgacgcatc tggggctggt
    40201 cacctggcgg gtcgtgttcg agcaggccga gcgccgccgg gtgaggtcca tcttcgcgcg
    40261 aatcgtatcg ccgaagatcg ttaatgagct gctggcggcc gaaagctttt cgctgggcgg
    40321 ggtgcggcgt gaaatcacgg tgttctttgc ggacgtgcgg gggtttaccg agttgacgga
    40381 ttcgagccag gaacgcgtgg cggccctggt gcgccgccat ggcttgaccg ggcaggccgc
    40441 cgaggattgc tttgacgagc aggcccggga aacgctcagc accattaatc tttatctggg
    40501 cctggtggcc gataccgtga ttcggcatga tggcacgctg gataagttca tcggggattg
    40561 tgtcatggcg ttttggggag cgcccactcc caatccccgg cacgcgctgg cgtgtgtgcg
    40621 ggcggccatc gaaatgcagc gcgcggtcga gcagttgaac cagcagcgcg ccgcggagaa
    40681 cgcgcggcgc gagcgcgaga atgtcgaacg cgcgtccgcg gggctggcgg agaaaccccg
    40741 gctgccccgg ctgctcctgg gcacgggaat taacactgga atggcgaccg tcgggttgat
    40801 gggatccgcc gccgaactgc agaattacac tgtgtttggc cgggaggtta acctggccag
    40861 ccgcctggaa aatgcctcgg ggcagggccg catttttatt ggacaatgca cgtacgaaca
    40921 tctgcggcgc gatgatcccg ccctggccgc cacctgcacg gaattgccgc cgcaagacct
    40981 caaaggattt cgctcgccgg ttaaggttta tgaagtcccc tggcgctccg cggaggccgt
    41041 tccgtctgcg gcgggtcttg cgcgccagga ttcctccgcc taatcccgct tgccgggctg
    41101 tcgccgcgcg gcggcggacg cgtcgccggc ggggcggggc cgcggcggaa gttggatttc
    41161 cggctcgtca tttgcggcgc gcatgctaga gtaggaggga tgcgtacggc gatttatccg
    41221 ggaagttttg accccgtcac caatgggcat ctggatgtgc tgcagcgggc gaccaagctg
    41281 ttcgaccagg tgattgtggc ggtggccagg agtgagagca agcgcccgct cttttcgctg
    41341 gaagagcgcg tgcggatggt ggtgcgcgcg acgcagcatc tgccgaacgt ggaggtggat
    41401 tcgtttgaga gcctgctgtt gacttacgcg gagcgccgct ccgcccaggc cgtggtgcgc
    41461 ggcttgcgcg cggtttcgga ttttgagttt gaatttcaac tggccctgat gaatcgcaaa
    41521 ttgaatgatc ggatcgagac catcttcatg atgccgaagg atacttatac ctttctcagc
    41581 tcgcggctgg tgaaggagat ggcgcaattg ggcggcgatg tcagcgcgtt tgtgccggcg
    41641 catgtgcggg aggccctggc cgcccgattc agttcccggc cggcctgacg aggagcttat
    41701 gccgctggtt gtaaatctgc gccacctgga ggcgcataac gtgcgcctgc aaggccaatt
    41761 atcggtggcc gagctggata ttgacacgcg ggacgaggtc atccaggtgg cccatccatt
    41821 ggactacgcg ctggaagtgc aaaaattgga gaatggcgtg ctggcgcagg gccgtttatc
    41881 cctgaaactg gagtgccagt gcgttcgctg cctcaaatcc tttcagtacc cgcttgaatt
    41941 gaaagaatgg acctgccatt tgccgttgca gggggaggat tgcgtggcgg tagttaacga
    42001 ttgcgtggac ttgacttcat tcgtgcgaga agacatgctg ttggagttcc cgcggcatcc
    42061 cttgtgtgac ccggggtgcc gcgggttgcc tggaatatcc aggggcaaag caaaaaatac
    42121 ccgccgccca ggtgaaagcg aaagggatca gtcggtctgg gccgagttag ataaactgaa
    42181 attttaaaga attatgggtg tacccaaacg aaagccatca cgcagccgtc aacgcatgcg
    42241 ccgcgcttac aatagcgcga tgactctgcc gcaactcagc acctgtccgc aatgcgcggc
    42301 tccttatgtg ccgcatcggg tttgtcctgc ttgcggatat tacaaggggc gccaggtcat
    42361 cacggtgacg gccggggcct gaccggtttt acgccccaac cgggttttcg ctgctttcgg
    42421 cttcagtatg cgaattgcag tggatgtcat gggtggcgac cacggttgcg gggtcgtcat
    42481 cgcgggcgtc aaacggactt tgcaggcgga ttcgagcatt tccgccattt atttggtcgg
    42541 caatcgcagc gagatccatg cggccttgcc gccgcgcggt tttcgggacc atcgcgtgcg
    42601 ggtggtgcat gccagcgagg tgcttacgat ggccgacaag cccgccgcgg cattgcggcg
    42661 gaagaaggac tgctcgatcg cccgagcgat cgaattggcc cgcgagggca aggttgacgc
    42721 gatcgtttcc ctcggcaata cgggcggcat tttcgccgcc gcgacgttta agctgggccg
    42781 gattcccggg gttgatcggg gcggcattgc cacggttatt cccacgccgg aacatgcgtt
    42841 tgtgttgctg gattccggcg ccaatatcga atgcaagccc ctgcacctgg cccattacgc
    42901 ggtgatgggc agcatctacg cgcgcgagat tctcggctat aagaatcccc gggttggcat
    42961 ccttagcatt ggcaccgaag acagcaaagg caacgaattg acgcgggagg cgtttcggct
    43021 gtgcaaactg ctggatttga atttcatcgg caacgtggag gggcacgatt tattcaataa
    43081 ccgggtggat gtggttgtct gtgatggctt tgtgggcaac attgttttga aaacgtgcga
    43141 gagcctggcc tcggccatgt ttgccatgct gaaacgcgag ttgacggcga atccgcggcg
    43201 tcagttgggc gctttgctgg cgcacaatgc cttccgcact atcaagcgtc gcatggatcc
    43261 cgaaggctcc ggcggggcgc cgctgctcgg cttcaacggc acggtcatga aggcccacgg
    43321 ttccgcccgc gagcgcgcca tcgccagcgc catccgcgtg acggctacga acgtccagca
    43381 tcatgtgaac cagatcattg cgcgcgaaat tgcgcgcgtg aatgagcgcc tggccgccga
    43441 tgaggcaacc gccgcagtcc tccaggcgca aaactagcgc gattatgcca attcagcgat
    43501 caggaagcga gtggcgggcg gccgccttca caaccctcca gccgttaatg gcgattgcgc
    43561 cggatgccgc ccgcctgggc cgctcctggt caaggtttat catcggctga tctattttta
    43621 aatgaacccg tcaactcctc cctctttcaa aaacccaagg gccaagtata attttcaggg
    43681 ccgcacctgc tccatcacgg gcgtgggctc ctacgttccg tcccgagttc tgaccaacgc
    43741 cgaattggag aagatggtgg atacttcgga tgagtggatc acgacgcgca cgggcatcaa
    43801 ggaacggcgc attgccggcc ccaacgagtt tacttccgac ctgggcgcgc aagcagcgct
    43861 ccgcgcgttg cagcatgccg gggtcagccc ggaggaggtc gagttaatca ttgtggccac
    43921 gataaccccg gacatgccgt ttccggcgac ggcctgcctg gtgcaacaaa aaatcggggc
    43981 ccatcgcgct gcggcgttcg acctggaagc cgcgtgttcg gggtttattt acggcctgga
    44041 agttgcgcaa caattcatta cctcgcgcac gtacgacacc gttttggtca ttggcgcgga
    44101 gaaactttcc acgattgtgg attggaagga tcgcaatacc tgtgttttat tcggcgatgg
    44161 cgcgggcgca gtggtgctgc agaatcgtcc caactcgcat gggctgttga ccgcggtaat
    44221 gggggctgat ggccgaaaag cggacctgct tttcgtggcg ggcggcggca gccgctgccc
    44281 ggccagcgca gaatccatcg ccgcgggcat gcattacctc cggatggaag gccgggaaac
    44341 ctacaagagc gccgtgcagg cgatggagac tgccgcggaa gaagcgctgc gccgctgtga
    44401 aatagacatc tcgcgcatca agtgcattat tccccaccag gcgaatcggc gcattattga
    44461 cgcagtcgta gagcgtctcg ggggcaagcc ggagcagctc tttgttaatt tgcataaata
    44521 cggaaatact tccgccgcgt cagtggttat cgccctggat gaggcggttg cttccgggcg
    44581 catccagcgc ggcgacttgg ttttgctggt ggctttcggc ggaggattga cgtggggtgc
    44641 ggctgttatt gagtggtagc agccggtctc cggactatcg ctggctaatt tgacgattcg
    44701 tttaggtgtg ctattctaat actgcagtat gagtgctaga agaagtgcca acaacgggtc
    44761 ggccaccgaa gtgaaagtcg aggcggagca aacgtgtttg acactttccc ctgacgcggt
    44821 cattgcccac aagaacggta ttgaatttcg cagttcccgg ccgtttgcgg tatggcggga
    44881 gatgaccatg tcgttgcagt ccgccggtgg cgacaaggtc tgctgctcgg gcgtcgttat
    44941 ttcttgcacc ggcaacaaac acaccgggta tcacgtctca atggttttga ccggcctatc
    45001 gcggcaggcg caagcgcgcc tgagcgcgat ggtctatgct cccttcaata gtttttgatt
    45061 tcgttaaaga aaaaatcact ccgccacctc gaaagaagtc cccaggtcct gattgcgcaa
    45121 tcagtgcgct gggaagctgt gaggaaatag cgtttggagc ctgcggcgta taggcgccac
    45181 ccaatctgca gccatcggca ggggcctccg ggatacaccc gatcaaacgc cgaaatacaa
    45241 ttcgggaaaa ccatcgaaaa gcgggattgc gagaacatct ggacgcccgg aagaaaattg
    45301 gctgctctgc gccctgaaaa caaccggcgt gaggcggcgt tggttttgcc ggatcaaaac
    45361 ctggtcccgg ttcttttcgt ccttggcttt ccgagcctgt tcgttatttt aaaagcgtgg
    45421 aaatatttca gcgtattttg aaaacggcgg ttgacggcgg agcttcggat gttcatctaa
    45481 agattggcac cccggtcatc tttcgcatca atcgccaact catcgctatc gaatgcccgt
    45541 tcccgactga ggaatggatg ggcaatgtgg tgcagcagat tacgccgcct catttgcgca
    45601 agcgcgtaga ggaagaacgc gaggcggact tttcgtacta cgtggcgggc atagggcggt
    45661 tccgcaccaa tctatttcag cagcgcggcc aatggtgcct ggccatgcgc tacgtgaaga
    45721 cccgggttcc cagtttcgag gagttgggac tcctggaaca ggtgcgtcgg atcgctgaat
    45781 ctccgcgggg cattgttttg ttggccgggt ccaccggttg cggaaagtcc acgacgctcg
    45841 ccgcgatgat cgagcacatc aacgccaatt tcaagaagca catcatcacc ctggaagacc
    45901 cgatcgaata cgtgtttgaa gataatcaat gcgtgattga acagcgcgag gtggggttgg
    45961 acacgctgtc ctttcaccat gccctgaagc atgtgctgcg gcaggatccg gacatcatta
    46021 tggttggcga aatgcgggat tcggttagtt tcacttccgc catgagcgcg gcggataccg
    46081 gccatctggt gctctcaacc ctgcacacca ccaatgcctc gcaatccatc agccgcatcc
    46141 ttgacttctt caaagctgat gaacgcgagc aaattcgccg tcagcttgcc gggaccatgc
    46201 aggcggttat ttgccaacgg atggtagcca ctttggctgg gggcatgacc ccggcgcttg
    46261 aaatcatgat caacacgccc acagttaaaa agttgatcga ggaaaaccgg ctggacaagt
    46321 tggcggccgc catcgagacc ggcaccgaag acggcatgat caacttcaac caggccctgt
    46381 tcaatctggt gaagcaggga aaaatcagcg aacaagaagc cttgaacaaa gccagcaatc
    46441 cgcaagcctt ggaaatgaat ttcaagggta ttttcctgga cgaagggcgc cggatcctgg
    46501 cgtaaagggg cggataggcg cgggacgggc ggaatccccg cctcctttgc cggcgcaccc
    46561 gctgtttcgg ataaaaccaa agaataatgt tctctgggct atccgcaggt accatagggg
    46621 agatggaagc ctgcgcgaca ggcagggaat gaaggcttcc cggacgagtc tttaacgggg
    46681 gacagccggt tctggtggtg cgtttgtccc ggcaggcacg gcattggtct cgggcggttc
    46741 gcccaacttg gagcgcagca ctgcccgcac ttctgccggt tcgccgccct ggggatcgag
    46801 catgagtttt cggatttcct ggtcggtttc cgcaaagggc cgcagcccaa tcgcggccag
    46861 tcgttgcttg ggcatctggc tttggtaagc ctcccaaacg cgttgcgcca gcaatcgaaa
    46921 gccagccgcc gggccgtctt cacccagcgc caggctgcga taggaactca ccagcaatcc
    46981 ttccacaatc ccctgggtac gatcgcggcc gccgctgtcg ctcacatcct cttgcacgcg
    47041 cgccacggca tactggtcaa gcgtcagggt ggcaggcaag gaatcgggtt tgccctcgag
    47101 cagcggttta ttcggatact gggtggacag gtatttgtac caatgagccg cgtcggccag
    47161 ccgattatag gtataaagga agtaaacggc atcgcgcagg aaattgcggt gcgccttttt
    47221 aatatgctcg cggttctgct ggtcctcctc ggcggcttgc tcgtaggcaa aattcacctt
    47281 gggaataatc gccaggttgg gaccaaattc gtaggcctgc gcaaagggat tcaaaatcag
    47341 ccgtccgcgg tgaaaactca gcagcattga ctggtaaatg acccggcgca gggtgatcag
    47401 gtcgtcttcc ttgattcggg tcgggttctc ccgggccttt tgcaatccca gcgccgccca
    47461 ataaaccgca tgcgcctccg gtaagcgcca ttccagcggc ccgtattgct cgtccacggt
    47521 cttcatgaac tccgggtcca ttttgtaggt ctcgcgcagg agtttggctt gggcttgcgc
    47581 ttctgcggtt tgcggatgga tcaactcgtc gagattaggc gttttcttgt cgaaaacccg
    47641 ggccatttca tcggcccact gctgtttgta atacgagctc gcgtcatcga ggttgtggcc
    47701 gagcttatgc tggaagaacc atcccaactc gcggtagagc agcgtttcgt tggggttaaa
    47761 gcgcaaacca tcgtcgcgca gcagttcgat gccggccttg acccaacgcc accgatcggg
    47821 aaactgcccc gggctggttt ccttgaactt gaccgagatg ttgtaggcca tattccaggc
    47881 ttgcacgagc cagacgtgaa cgaaatgcgg ttcgagcttg gtgatccagt ccgccagttg
    47941 cgccatctca aagaacttgt cctggtcctg caaatcggtg gcgcgcatcc acaatgcgtt
    48001 ggaaatcaac cctcggaagc cgcccagcgc aaccgtggtg aaggcgagca cgggcggcgc
    48061 gttttcgagt ggctgcactt gagtcagccc cagcagatcg cggtcgtggt tgatcagccg
    48121 ttgcgtccag ccggcgcccg ccaacagcgc ggcggcaagc aataacagga gaatcctttt
    48181 gagcgggaag ctcattgggt ttgggcggcc gccagttcgc gccgggtaaa gatcaggatt
    48241 ccaatcaaac cgatgcttcc actgagcaac agcacgattt gcccaaacgc ccggccaagc
    48301 tcgccccagg cgacgctccg gcctgtgctg agcgagtcaa tgggggaaaa atccttggcc
    48361 aggttgattg ctgccagcac ggtccgaaag accgggatgg ccaccacatc aacggaagag
    48421 tgccccgcct ggccatgctc cgaatcaaaa cctcccaggg tcccctcctc taccgtgctg
    48481 gcgatggttc cgctggatag cgccagggtc aggatggcca gcgaaaagaa cgccgctacc
    48541 gggaaggaaa gcaggctggc tgaggccaat cccagggtgg ccagcaatgc catccagcaa
    48601 aagatgattc ccagcccgcg cgcgtagttc aggccaaatc cgccctggcg atagagcacc
    48661 tccaggccat cctcgagcgg gaacagcagc gccgtttcgt tcaggttcac aaaacttacc
    48721 gtcaatatgc cctgttcgtc atagtatgcg gggacctcga actcatggaa agtatccggc
    48781 gccaaactca tggcgtcact gtgccatagt gaggtctttc gcggcactcc caccaaccat
    48841 cgcccctcga aagttccgga agcgctcttg tttgcggcgt tgaatttgat ccgcaagtga
    48901 accatctggc cgggcgctat gcgtccggca ttgccgaggt ccacctccca aatgtgcata
    48961 taccccggcg gcaccacctc atatccggct ttgacttgtt ccaggatctg tttgcgcgct
    49021 tcggaaatat tggccagaat atcggggtac ttctgccgtc gttcccggag caaccgatcc
    49081 gcctcctgcg caatttgccg gtcaaagctc gggggtttga tcgagccgcg cgccaccaga
    49141 acctcgtggc gaaggatttt ctgctcgtcc accggcagcc gggtggcgcg ccattgcagc
    49201 aggccataga cgctggcgcc ggacagggcc agcagggcgg cgttcaagga catgattccc
    49261 agccacttgc ccagccagat ttgccaccgc gcaaccggtt tggtggcgac gacttgcagc
    49321 gtgcattcct caacatcccg cgccagggtg ccgcaggcca gccagagcgt ggaaagcccg
    49381 agcagcgcgg taatcgagct gagcgtgtaa gtcagaagga tttgcgtgaa tccgcgcgcg
    49441 gtgccatcat ccttgatcaa caaaggcagc cccaccaccg aacccagcag caagccggcc
    49501 acgaccagaa aaagccggaa ccggaatgcg gctttccagg tcaatcgagc aatggccagc
    49561 aggggctgca tgaatcaaga aagccgcggc ggcggcccgc aattattctt tctgttttcg
    49621 caccagggac gccagttttt cattggcttg acgcagctct tccgtggtgg acggcttttc
    49681 cgctgccggc ggaggcggcg gcggagccgc gggttccgac ggcttgatca gggcttccag
    49741 tttttgccgg tccacggttt cgtcgggttt cggcgccgga gtgggcgcgg gccgggccgg
    49801 cggcggcgcg gccagccgtt ccaggatttt gtccgacgcg ggcgcggctt cagcctcgcc
    49861 ccgcaggtaa gcggccaccc gtgcgccgga tacggcgccg gaagtttcgg cctccgccag
    49921 ccgcgcgttt tgcaccactt cgaggaagta gcgttcgaga ttttgcgtcg gcgtatcaat
    49981 gctgatccgg gattccgcca cgtcctggcg gatgaaggcg agcacgcgct cgagggtttc
    50041 gcagggcaga gcgggcaagg tgaggcgaat cgcatccggc gcggccagca actccttcag
    50101 cgagcccatc gcctgaatcc tgccgccata ataaatgacc acccggtcgc aaacatcctc
    50161 cacgtccgcc agcagatggc tgctgagaag gacggtcttg ccgcggcgcg ccagggccag
    50221 gatcaaatct ttgacttcgc ggcaaccgat gggatcgagc ccggaagtcg gctcatcaag
    50281 gatcaccaga tcggggtcat tgatgagcgc ctgcgccagg ccgatgcgcc gctgcatgcc
    50341 tttggagaac tcgcccaccg cccgcagccg ggcctggctc aaacccacca tttccagcaa
    50401 ctgttccgtc cggtcgtggc gatcatggcg gggcaggcaa aagaggttgc cgaagaagtc
    50461 cagcgtctcg ccggaattga ggtagcgata aagatacgat tcctccggca ggtagccgat
    50521 gcgagccttg gtcgccacgt ggctggggga gtgtccgaag acttcaaggt gtcctttggt
    50581 gggatggagc aaacccagaa tcagctttac ggtggtggac ttgccggagc cgttgggtcc
    50641 cagcaagcca aagacttctc cgcgccgcac atcgaaatca acattgtcca cggcgcgggc
    50701 cttgggccgg ccccagaaat ctttgaagac cttggtcagg ccgcggatgg aaaccaccac
    50761 ctcgccggtg gctggccgcg ggccagggtc tgctatggtc gtgctcatgc caattacctc
    50821 gggctacggc tcccgaaccg ccaactccgc cgcttcacgc catttggggc gccagccccg
    50881 gacgggtgcc gggcgcggct ggttgctttg gtcccggcgc ggcgggttcg ggctggtaat
    50941 gctcgagctc ctcgcccttg cgcacgaggc gcgcgctgtt gaagaccacg atcagggagc
    51001 cagccgtatg cattgccgcg gccagcacgg gcggcacgta gttcatcgcc gagagcgcca
    51061 tgccgccaat aatgaacacc gcgccgaaca ggaagttctg attgatgacg gcgcgggtgc
    51121 tgcgcgagag tttcaccaga aacggcagcc ggcggaggtc gttgttcatt agcgcgatgg
    51181 tggcgctatg aatggccact tcgctgcccg ccgcgcccat ggcgatgccg atatcgccgg
    51241 cggccagcgc gggggcatcg ttgacgccgt cgccgaccac cgccacctga tagcccttgg
    51301 ccttgatgct gcgaacgaaa tccaccttgt tctgcggcag gcactcgccc cgaacctcct
    51361 cgcagccaat ttcacgcccc acgcgaaggg ccacgggctg ccggtcgccg gaaatcatgg
    51421 caatgcgccg cacgccgctg gccttcagtt cctgcagcga ctcacgcgcc tccgcgcgcg
    51481 tctgatcctg caatcccacc cacccgatgc accggccatc gcgcgccacg aaaatcaggc
    51541 tccagccttc ggtttcattg aggtccaccg attggagaaa atcacccttc acgccattat
    51601 cgtgcagcca ttgcgcccgc ccgaccaata cttgcgcgtc gccgacgcgg gccttgatgc
    51661 cgcgcccggc ggtttccgca aaatctttgg gctcggtcag cggcacgctc gcctcggcgg
    51721 ccaactgcat cagcgccttg gcggtggggt gattgctgta gcgttcggcg gacgccgcca
    51781 cgcgcagcaa ctcggccggc gtgaccccgt ccaacggcgc cagtcggctc accgccagtt
    51841 cgccggttgt cagcgtgccg gtcttatcga aaacaaaggc attgagtttg gccgccagct
    51901 cgatatcggc cacgttcttg atcaggatgc ccagccgcgc cgccgccgag agtgcggcca
    51961 ccatcgcggt cggcgtcgcc agaatgaaag cgcaggggca cccgaaaatg attacggaga
    52021 tgacgttgtc cagattgcgc gtgaaggccc agaccagcgc cgcaatgacc agcaccagcg
    52081 gggtgtaaaa ccccatgtac tggtccacaa tcttcataat cgggagcttg gttttttccg
    52141 ccgccagaat cagctcgcgc acgcggccca gggttgtgtc ggtgcccgcc cggttcacct
    52201 tgatctccag aacgccggtc aggttctggg tgccggcaaa gacttcgtcg cccggtttct
    52261 tgtcggcggg cagcgattcg ccggtgatgg tggcctggtt gaacgagccc tgcccccgga
    52321 ggatgacgcc gtcggccgcc acgttatcgc cggggcggat gcggatgacg tcgccgatgg
    52381 ccaggtcttt ggcggcgacc tcctcctcgc cctgcggggt gatgcggcgg gccttggtgg
    52441 gcgtcagctt gatgagatgc tcaatggagg cgcgggcgcc ttcggcggtg cgggtttcaa
    52501 tgatctcacc catcaacatg aaaaaggcca ccactcccgc cgtcttgtat tcgccggtgg
    52561 cgaacgcggc cagcacggca atggcgacca gttcgttgat gctcaggatg ccgccgcgga
    52621 ggtccttgat ggcggtcagg acgatggggt agcccagaat gacggcgcca atcaaaccgc
    52681 tggcgctggc aaccgtatgc ccctgcgcga agaaccaatc caccagaaac gcgttgaata
    52741 cgaagacgag cccgaccagc gtctgccaga gccgcaccgg ggtatgctca tggtggtggc
    52801 cgcatgtcgc gcacgattca tcgtgctcgt gccggctcag gagagaagtg atttgcatgg
    52861 cattaacgaa agcaacgccg gaggcggcgg gccgcgccac tcatggcttg gcctcctccg
    52921 gtttgggttt gggaacctcg cggtttagca gcagccgcaa ttctttgggt ttgcccgcgg
    52981 ccccctcggc caggaagatt ttatcctgca cgttggtgag cacccggcca agcgtttcgg
    53041 tcaggcgctg ctcctcgaac agggcggggt gccggcggta ctgggctaga atctcctgga
    53101 agcgttccgc ctgggctgaa gtatcgctca cgaggcgggc gcggtcggat tgggccaggt
    53161 tgaccaggga ctcggcgtcg gcgccggcct tgttggtgac ctggttttcg taggtgcgcg
    53221 cctcgttaag cagcttgctg cgcatgactt cggccttgag cacattggcg aaggcgtctt
    53281 tcaactggcg gggcggcacg ctttgcacca cgcattgctc gaccaccacc cccaactcct
    53341 ggagttccag cagttgcgtg gttcgccggc gcaccatttc ctggaagctg gcaatatccc
    53401 gggtgaggat atcgtccacg ttgaaccggg ccgccgcata gagcaggcca ttgtccaacg
    53461 cgctctgaac cgcgttcgag gcggcgacga agccaaagac gtactgaatc gggtccttga
    53521 tgcgataagt cagcgtggcg cggctgtgaa tgatattggt gtccgcggtc agcacgtagc
    53581 catccacgat ggggttgagc gtggcgcccg ccggcggttc gatgcccgcc agcaactgct
    53641 cgggggtcac cgcgtaccag cccacggtcg agctgacctg ctgcaaaccg gaaatcgaga
    53701 ccttgatgct ttcgtctatg ggatagggga acgaccagtg cagccccggg ccgagcaagg
    53761 ccttttcgcc ttcgccccgg gccttgccaa agcgcagaat gatcgcccgt tcctggggac
    53821 ccaccgtgaa gaagccggac gcaaggaaga gcagcaccag cagcaccatc accactttga
    53881 cgatgacaaa gctgctgcgc agggcttccg ccagcgcctg cgctccggca tccagcggca
    53941 tttccggcgg cggggcgggc tccggcggcc ggtcgtggga atgagcgtgc ggattcatgg
    54001 ccgggtcatg gttggagcag gttggtcgag gcgccccgga acaggtcaaa cggcggcgta
    54061 tgctgatcaa agataagcac cgagcgctcc ttcagcgaat cttccagggc cgacaggcgg
    54121 aagatgaacc gggccaggtt ggggttttgc tggaagacct tcagggactt ggcggcctcc
    54181 gcctcgccct tgcccttgat ttccgtcgcc tgcccttccg cgtcggccag catctccgcc
    54241 gcccgccgat cggcatccga gcggatgcgc tgggcttcgg cttcgccctc gtattgaaag
    54301 cgatcggcaa ggacctgccg ctccgaggtc atgcgctcga agacggactg ggtcacgctc
    54361 tccggcagtt gcagccgttt aaagcccagg aagacgatct ccagtcccag gttattggtg
    54421 cggagttggg attggatggc cgccaggatc tcgttttcaa tggcgacaaa gctggcgccg
    54481 ccatcgctgg tcgaaacaaa gtccgccagc ggatgccggc cgacgaccgc ggttttggcg
    54541 ttgcccagcc actggtccag gagcgtttcg gccgccccaa tcgggttggc gctgtcggcg
    54601 aaccggggga agaaagccgc cgggtctgac acctgccagc ccacataaac cgaggtcagg
    54661 aggttgaaat tgtcctgggt caacccctcg gtgagccgat cctcgaagtt ctggacgcgc
    54721 ttgtcaaaag accagacctt ttgaataggc cagggccact tcagatacaa gttgggctcg
    54781 gtcagggggc gcgtgggctt gccgaaggtg gtcacgacgg caacttcggt cgtgcgcacc
    54841 tgaaaggtga acagcagcag cccgaagatc aggatcaaca ccaggccgat aacgagggtc
    54901 aaagaattgc gtttcatgaa aattactggg ttttgggcgc gggcacgctc aggttcagca
    54961 agctctgcgc gatggattcc tgcaaatcaa aggtaatcac gtcctgcgtg ttcgtggtca
    55021 gcaaaaggta tttccgcgcg ggggccgtgg cacggacgaa cgtctgcaag taagcccgct
    55081 cggtgtaaac cgacggcgcc gcttcaaacg cgggaatctg gttggtgaac agcgccgcct
    55141 gggccagcgc gcggatttcg cgcgcccagc gctcggcgct ggcctggttg gtcaggctga
    55201 cggtttgccc ccgcgtcagg gcgttggtcc ggatgtcgtc cgcgcgcgcg gccagaatct
    55261 tcgcctgctt cgtctggctg gcggcgacga ctttctcata ctccggcgcc actttgatgg
    55321 gcggatgcaa atcgcccagc gccaccgcga taatccggac gccaagcccg cgttcgttgg
    55381 cggcggcctg gatgcgttcg ttcaggtcct gggccgccgg gccgcggccc tgcgacataa
    55441 tgtcgcccat atccacgccc accaggtagc gcagcacctc gcgcgtgctc agatgctcca
    55501 gcagcgcggg ggcgtcttcg ttgcggtagg cccaatccac caggttggtg atctggtaat
    55561 agatcggcac gctgacggtt agcaggctca ccggcggcgt gcgcttgccg acggccgggg
    55621 tgacggcggt atgcgaaagc tgctcccggt tggccaccag aaagttcgtt tccaccgagt
    55681 gcccctggcc ccacaagaca aaccgggaat tttccttcgc cgggtcgggc agggaaccga
    55741 tggtcaggct ctgaatgcgc tcggtctgaa agcggtaaac ccggtcaatg ggccacggcc
    55801 atttaaagtg cgcgccgggt tccagcgggg agcggctctc cacccgccgg ccgaagcgct
    55861 cgagcaatcc ctgctccccc gcctcgataa acaccacgca cgtggacagc agcagcgccc
    55921 ccacttgcag cagaatgagc cagcccagcg cccgctcaaa gaagcggtag aaccaggttt
    55981 cggaaacctt gaacccgaat tggtaatcca tcgcctgcgc ggcggtggtc accagcccct
    56041 ccggctgccc cagcaacccc accagccggc tttcataaag cggctgcccg actttgcccc
    56101 ggacccgggg acggtagatt tccagcacca gttgcaccag ggtttccagc gcgatcagcc
    56161 ccagcaaccc gcacagggcg cgggcgacat agaaatccgt ctgcgcaaac ccggcccaaa
    56221 cgccgacaat gcccagcgcc accaccaggc acagcaccgc gttgcaaagc aaataactgg
    56281 cccctggccg caacaggcgg tgacgctgca ggcgggcgac cgtggcggaa aaccggccca
    56341 gcaggaacag gattagcgcg aagaggccaa acaaggccag cccggcaatc ggctccttga
    56401 gctcgatttt cgtggtgctg cgcgacagcc agcgccacag gaaataagcc ccgccggcct
    56461 gcgccgcaaa caggaccacc gtgaacgccg gcacgaagaa ccgctcgaac tgctcgcgcg
    56521 cgcgctgcgc cggaaacagt tccgccgtct tggcctcaaa catggccgac gcgctgcggg
    56581 tccgggccag ttcgtccaac tcgagtttct ccagccgctc ccgctcctcg agccgcatct
    56641 ggaaccaact gaccagcgcc accagcgccc ccaggcccag atacagcgcg cccacctgcc
    56701 ccgccagcga gccggcatac cgcgccgcca gcactcccgc ggcccccgtc acgatcaagg
    56761 ccagcaggtt gatcagcccg ttcctctgta gggtcttatc catgaagaat taactcaatt
    56821 cgcgttgctc aaataacagg accgccaata ccagcgcggc gcccacatag gcggccatgt
    56881 aagcgaacgc cttgccggca tagccccagt gaaaggtgct tttgccctcg gtcaaggcat
    56941 ccgccagcca gaaaagctgc cagtttggcg tcgccgtgta aagcaccgac gcccaccatg
    57001 ccccccccag cccggcctgc cgcaggcgcg cccggtccgg ctggctgatt cgcttccgct
    57061 ccgccaaatc gagctggtca ttgtgatcca cgtcatactt ggccacggtc tcccgcaata
    57121 acaccttttg tttctccgtc cagcggccgc tgtggagctc ggccgccagg tcgcgccgcc
    57181 agaccggttc cgcccgccgg ccaaacaggt aatccgacat gagccccagg agaaaggccg
    57241 cggtgcaaat cgccagcgtg ggcaccatgt ccagccgcgt cgagcaggcc agtgccaggc
    57301 tcgccagaat ccaaagcgca aagagaatca gcagccccgc cggcaccagc cgccagtcca
    57361 ccgagcccgg ctcgttcacg gaccgcagtt gggtcgtgaa ctgaaagatc acaaacgccg
    57421 ccagcgtgac cgtgatcagc agcgcaaaaa cagcatcgct cacaaaaggc cggcgcagga
    57481 agaaattgct gaagcccccc accgcgtagg cggccaccac cccggcggcg aagatgccga
    57541 ccgccgacag gtcggttttg ccatagctgt caaacgtcat ccggctggca atcaacgccg
    57601 ccaccaggtt cacatacgtc agcaccgcca gcgccgccgc cagacccaca tacttcgcca
    57661 gcaaaaactg cgcccgccgc acgggcttgg acagcaccgc cagcgccgtg cccgtgtgaa
    57721 tctcccgcgc cagcgaagcc gaggcgctca acaccgcccc cagcaacccc gccagcagca
    57781 tcaccgccag cgtgctgttc ttgaccagcc gcggttcgtc cccaaacgca aaatagtagg
    57841 gcgtcgccag gaagatctca aaggccgccg aacaggtcat cagcagcaga aaaatcggct
    57901 gccggaccag ctccataaac ccgttggacg ctatggtgac gaactgtcgc atcagtctta
    57961 atcagtcgcc cattctaacc ttttggcata ttaacgcaaa tgcttcttga gccccggttt
    58021 catgccttca tccctggggg cgccccggtt tcttccggtt ccgcaaaacc ggggagggcg
    58081 agcttccaa
//
LOCUS       DAOPQG010000006        52816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_811_length_52816_cov_15.441671, whole genome shotgun sequence.
ACCESSION   DAOPQG010000006 DAOPQG010000000
VERSION     DAOPQG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 52816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52816
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_811_length_52816_cov_15.441671"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(591..1862)
                     /locus_tag="PKI07_01215"
     CDS             complement(591..1862)
                     /locus_tag="PKI07_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOC49699.1"
                     /translation="MINNRRTISGLLTVLLWGAWALEAPANGFRLGGQDAFATARGEA
                     FVATADNASAVFYNPAGITQLEGLNFRGGISGLYYEPRFTPPGPGRTNTYHIKDSWAA
                     VPQGFATYTPRDWPLSFGVGLYAPYGGSISWPQNTGFRSIATDASLTYATINPVVAFR
                     PASWLSLGGGLMINYVDLEMEQGLRKLSPLLKDYFRFKGDGWSESYNLGVLFQPHRKL
                     SLGATFRGAAPVKLEGNTDFLRQPNIKQGRRSAHMELDFPLTAVVGISYRPTPKWNLE
                     FNADYTEWSSFGKTTLYQSPAPPYPISRNVDVKLYWQSSWIYSFGVTRYFEPGWHLSA
                     GYAFSENSVPNAYYTPLAADLDRHFFSIGGGFRGKRYNFDLAYQFGYGPSHKVTGSKP
                     SSQPALSSGERADGTYDFLSHAVMLTVGARF"
     gene            2506..4020
                     /locus_tag="PKI07_01220"
     CDS             2506..4020
                     /locus_tag="PKI07_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC49700.1"
                     /translation="MSAENPAASENTKQTTACSRRDALKFLGAATAGLAVAAAPPAMA
                     AAGPAKGANPDGGAPGRGISRETLSSRGSAQGGILPVKYEVAVCGGGPAGLMAAIAAA
                     RNGAKTILIERYGFLGGMATQSLVSPLSVFNKNRKRIIGGIPLEFAELMETLGGADTT
                     YPSGNIHFDPEIYKLAAQRMVFASGGSLILHSYISGCLREGDGNGRITGIVIENKSGR
                     QKIEARYFIDCTGDADVIKFAGLPYVMGDGESHELQPMTLWFRLGGVDTDRLENMEMR
                     ESGKRHVNSRIRDALLKLGNEAPVPQFGGPWMFTTFRKGEASVNMSRYAGNGTDVLSL
                     TQAECTLREDVFKLVKLLREHFPEFKNAYLIDTGTQVGIRETRRITGLYEMTLDDIMA
                     PKDFPDTVAKGGHPVDIHKAGSREQDLRAVKTGYNIPYRSMIPVGSDNLIAAGRPICA
                     AREAFASIRVQATCMALGQAAGTAAALCHQKDIPVHKLDGAELNGILRKQGAVV"
     gene            complement(4226..5116)
                     /locus_tag="PKI07_01225"
     CDS             complement(4226..5116)
                     /locus_tag="PKI07_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOC49701.1"
                     /translation="MKPTSPNPQPERSGVGTRPPPPASPSFSRLFRPALRYCGVYAAL
                     WKNSVAREMSFKANFLLWILVELLWFAMQLCFIGVLYLHTEHIGTWTQWQVVLLIGAS
                     HFVQQIFQAFFLINCTNLSELVRNGKLDFLLLLPVNTRFVVSLRQVDLGAFVNAASAI
                     AVMIYAARQLQLAPSAGQIAGFLALCAAGVMIHYSLMFLLASISFWTVRAQGIVWGYY
                     NLFNVARLPDEAFGGLFKALFTFALPMLLVSNVPVRLLADKLSSPGPVLLLLGMSAVC
                     FAVSELGWRFSMRRYTSASS"
     gene            complement(5118..5924)
                     /locus_tag="PKI07_01230"
     CDS             complement(5118..5924)
                     /locus_tag="PKI07_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOC49702.1"
                     /translation="MKKYWHVINIGIQNSLTYRFNFLARALFGLVPLIAMLYVWQKIY
                     AGKGAGTSVGSYSLAGMISYYLLITVVDALTAVNEDDWLIAADIKDGNISQLLLKPID
                     YLWYRLCLYLSGRLTYLAVAFIPLALFFACFRSYLEPPADGVALAAFLLSTALTALLQ
                     FFMSYAMAMLAFWVLEVSTFIFILYAFEYIASGHLFPLDILPRGLEQALYFTPFPYQL
                     YFPVSVYIGKAAGMDLLRGLLVQASWVIAAYGAARFAWRRGIKHYSAVGG"
     gene            6038..6508
                     /locus_tag="PKI07_01235"
     CDS             6038..6508
                     /locus_tag="PKI07_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49703.1"
                     /translation="MITQKRCPFCQTPAVMGCPHLAIAVEGRDFVRRCVELCQGQKQW
                     RALAELRQRQRQATGEWTPERDDYTWLETAFCEEFLKPLSWFGGLEHEWRIGPKGNQA
                     GFLVLLWSKDPRRLWWQLRDELERQAQEPFPGKRRKVKGQSPRAGRRDGDLRLL"
     gene            6551..7639
                     /locus_tag="PKI07_01240"
     CDS             6551..7639
                     /locus_tag="PKI07_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOC49704.1"
                     /translation="MIRNIIFDWSGTLVDDLPAVWQATNYVLAQARQPEMSLEQFRAE
                     FCLPFTIFYDRHVPHIPMAQLEEWFHCQFRQVQASVRALPHAREFLEFCRARQFRTFL
                     LSTVHHEYFDIQSAATGFGPFLDKPYLNVWDKRQKIHEILAENQLAAEETLFVGDMQH
                     DIETARHGGIHSCAVLTGYNTLDQLRQAEPDLIVEHLGELREALERNGFSLKASPRQV
                     SEDRLPVATVGGLIFNARGEVLMIRTHKWSDLWGIPGGKIKWGETAIEALRRELREET
                     GLEVSDIRFVLMQECIHSKEFYRAAHFILLNYTCRCVGEPQVRLNEEAREWRWVTPAQ
                     ASEMPLNQPTRTLLSAATRLESQTKKHG"
     gene            7632..7988
                     /gene="folB"
                     /locus_tag="PKI07_01245"
     CDS             7632..7988
                     /gene="folB"
                     /locus_tag="PKI07_01245"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOC49705.1"
                     /translation="MAKITIVDLEVYYCVGVTDEERAQPQRLLLTVDMNFDFSSAAVS
                     DRIERTINYQTVAEDLLRFGQGRSWKLVEKLADNIAERVLAEYKPQGVLVEVKKFSIP
                     QARFVSVSLGKTRPSR"
     gene            complement(8027..8473)
                     /locus_tag="PKI07_01250"
     CDS             complement(8027..8473)
                     /locus_tag="PKI07_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOC49706.1"
                     /translation="MKPTGIVSDILSLKGRAAWAIAPEATVFEAIQLMADKNVGAVLV
                     TEGGKLVGIISERDYTRKVVLRGKSSKTTAVREILSSQVIYVSPTHTVEECLRLMTDR
                     HIRHLPVLEGDKIVGIVSIGDLVNWIISAQHSTISQLQTYITGLPG"
     gene            complement(8528..9304)
                     /locus_tag="PKI07_01255"
     CDS             complement(8528..9304)
                     /locus_tag="PKI07_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOC49707.1"
                     /translation="MMVTESLGLVIAAMAGGAVNAVAGGGTLITFPVLLLFGTPPVIA
                     NATSTLALVAGTSGGIYGYREHLGPVRQWLWRFAPVSVLGGVLGAILLTCTSNRVFSK
                     LVPFLILFATLLFLAQGVFRRFGSPGVHTSSKGKHRAVWGAVFFQFAVAVYGGYFGAG
                     IGILMLASLGFMGLSHIHEMNTLKTILASLINLVAAGWFIGAGLIHWPKAGVMTAGAL
                     FGYFLGAHYAQRIPPQQVRLIITLIGFIISVVTFYEQFCQ"
     gene            complement(9378..9464)
                     /locus_tag="PKI07_01260"
     tRNA            complement(9378..9464)
                     /locus_tag="PKI07_01260"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9428..9430),aa:Leu,seq:caa)
     gene            9620..11119
                     /gene="zwf"
                     /locus_tag="PKI07_01265"
     CDS             9620..11119
                     /gene="zwf"
                     /locus_tag="PKI07_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOC49708.1"
                     /translation="MEPCVIIIFGASGDLTARKLVPALYHLFKEKQMPPAFSVVGVAR
                     REKTDASWRTELRSALDRFSRTQPVDEAVWQDFAQHLQYCQGDLTAAATYEALRKRLA
                     AAENELLRRNLLLYLATSPSQFGEVVEQVYRAGLLHKEGGAGWQRVVVEKPFGHDLPS
                     ARQLNQELTRFAGEHQIFRIDHYLGKETVQNIMMFRFSNAVFERLWNRESIDHFQITV
                     SEKLGVGQRGGYYEEAGALRDMVQNHLLQVLSLVAMEPPVSLAAEAIRDEKVKLLKSI
                     RPLSEGDMARQVVRGQYSAGIVAGEQRLGYRQEAKVNPNSNVETYVALKLFIDNWRWS
                     GVPFYLRTGKNLPLSTSEVRVQFRRTPHVLFAAQCGQKLDPNALTLHLQPHEGISLRF
                     NGKVPGTSVAVRPVRMHFSYDAEFGAYTPEAYERLLLEAMAGDATLFIRRDEVEAAWQ
                     FVDDIRRAWEHKPLTDREFYAAGTWGPVAAEDLLAQNGHAWREPLVVRV"
     gene            11186..12472
                     /locus_tag="PKI07_01270"
     CDS             11186..12472
                     /locus_tag="PKI07_01270"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOC49709.1"
                     /translation="MTLTEQMVQLARQAKTAARELARLTPDDKNACLLAMAGALEQNK
                     ADIKNANARDMEVGAGMGLSPAMLDRLKLNDRRIISMANGLREVAALPDPIGKVLDER
                     VRPNGLKLRKISTPIGVVVIIYESRPNVTADAASLCFKSGNATILRGGKEALNSNQAI
                     ARIMIEAGKRALRGFPECAIQVVPTPERAAIKELLSLTQYVDLCMPRGGEGLIRAVAE
                     CSKVPVIKHYKGVCHVYVDGAADLKMAEAIALNAKIQRPAVCNAMETLLVDRKVAGVF
                     LPVVAHRLGEKKVQLRVDAAAGMILKSESENPTFEIRAATEQDWFNEYNDYILNVRVV
                     DGVRQAIDHINHYGSAHSDSIVTQDETCAQQFLAEVDSATVYWNASTRFTDGGEFGMG
                     AEIGISTDKVGARGPMGLDELTSYKWIGIGSGQVRT"
     gene            12514..13851
                     /locus_tag="PKI07_01275"
     CDS             12514..13851
                     /locus_tag="PKI07_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49710.1"
                     /translation="MNSAQANAPPTEAAERARFVYEHLPAEGLFAGQNWRISPFPCPL
                     GVALVRELETLGRVLLQFYRAANLLYRRSVEGRQPAWVARWLDLGKPSALIQLQRSPA
                     LKQALPRVIRPDLLISDHGLSVTELDSVPGGIGLTGWLNQTYSATGAEVLGGPDGMLR
                     GFAGIFGDAPNVHIVISEEAATYRPEMAWLAEQLGASRFQVRDAQFSAFADGDAVYRF
                     FELFDLANVPSAKDLFARAAARRVHLTPPPKPLFEEKMLFALLWNRNLRGFWRQELGE
                     NFYQRLLRLVPYTWLVDPTPLPPHAAIPELNLSDWHQLKELSQKERGLILKVSGFSPR
                     AWGARGVFLGSDLAQADWAIVVDEALANFARSPCVLQRYHKPRLIQAQWFDFTRQQLV
                     PMQGRLRLCPYYFVTGDADAARVEMGGVLATICPANKKIIHGMKEAILAPCSL"
     gene            13848..14492
                     /locus_tag="PKI07_01280"
     CDS             13848..14492
                     /locus_tag="PKI07_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOC49711.1"
                     /translation="MSLFEYIFLAASSLFVIVDPFAAVPAFLAMTPHDSAEQRIKTAR
                     LASCVSAGVLLAFAIAGKWIFKFLGITMPAFQFAASIVLLLVALDMLRAQRSRVQETG
                     EETAAGLEKADIAVTPLAIPMLAGPGAISTAILLHNQAANLGQCAALYVCIAVVCFAS
                     YWVLRFSARSTRWFSPIAMRITTRIMGLLLAAVAMQFMLNAVRQLNPAWFPPAA"
     gene            complement(14628..14771)
                     /locus_tag="PKI07_01285"
     CDS             complement(14628..14771)
                     /locus_tag="PKI07_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49712.1"
                     /translation="MTTSCFEPASGMVLLEIPERPSFWESARCPQDGGRVLYNDHTEE
                     FKP"
     gene            complement(15328..16587)
                     /locus_tag="PKI07_01290"
     CDS             complement(15328..16587)
                     /locus_tag="PKI07_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49713.1"
                     /translation="MAARTFVGFGFGPIQSALFLYEAFRSGHFSRLVVAEVNEAMVQA
                     VRSAAGRYTINIALPDRIEPATVSGVELYNPAVPEDRRQIVQAIAESDELATSLPSVA
                     FFDRDHPTSVARALADGFALRPTPRPTVIYAAENHNHAAELLRQAVEKYCPPPTLQGI
                     QFLNTVIGKMSGVITDPATLARLKLTPLTPAVPQAVLVEEFNHILISQITLPGFTRGI
                     DVFIEKPDLLPFEEAKLYGHNAIHALIAYLAARRGLTAMSDAAAHLDIMATARAAFIN
                     ESGAALIRKHAALGDPLFTPAGYAAYADDLLARMVRPTLNDLIARVTRDQVRKLGYDD
                     RFFGTMRLALRHGIRPVHLAQGAAAAVRSLIDQWDGLGQAVAALPRPTASWSKESVGN
                     LLRAIWSTKAGPEAPSLIDLTWESGMA"
     gene            complement(16591..18225)
                     /locus_tag="PKI07_01295"
     CDS             complement(16591..18225)
                     /locus_tag="PKI07_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005869465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOC49714.1"
                     /translation="MPNTALPTTQYALQLVGPGQLTLNPAKPVIPPGPCQVLARIECV
                     SLCFSDLKLLKQFDQHPRKGAVVKGISPEALREIPSYVPSSKPTVLGHEVTLRIVAVG
                     AKVKHHSVGERCIAQTDYRDLPTAGSASAFGYNFEGALQEYVLMDERVIIDPTGERFL
                     IPVSDQPAAAAVALVEPWACVEDSYVNLERQTLKAGGRLLIVADDPALVSLDGLPHAP
                     AGPPAEVLRHTPAQVGTLADQSFDDILYFGASKAVLEVLNDKLAARGIINIVLGGRRI
                     GAPVNIGVGRIHYGMTRWIGTTGSDPAQSYQVIPRTCELRSGENFVVIGAGGPMGQMH
                     VIRALCSGVPHLTVIGTDMDDARLASLRQKAEPFARANGVTLKMVNTAQTPLPGKYSY
                     FALMAPVPALVANSIRDSQPGCLINLFAGIPAPTKHELDLDTYIANRCFMFGTSGSTI
                     EDMKIVLRKVTSGQLNTNCSVDAISGMAGARAGIAAVENRTLAGKIVVYPMLYDVGLI
                     PLADLSRHYPTVAAKLEHGQWTRAAEDELLRVAAKA"
     gene            complement(18244..20292)
                     /locus_tag="PKI07_01300"
     CDS             complement(18244..20292)
                     /locus_tag="PKI07_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC49715.1"
                     /translation="MASRRQWPVFTKSDTPLEALVKLSRFYGADPEFVLAGGGNTSVK
                     VGNRLFVKGSGHALATISPEGFVEMDRLQLESLLHSSLSSNRLKREAEFKAATLAARI
                     HPEKRQRPSVEALLHHLMPGRYVVHTHSTLVNMVTCCVKGPELARQLAAEVVWVPEVD
                     PGYILSRTLWQALTDYQKATGRSCPRAVLMQNHGLAISGDTPAQIRRHTDWLVRTLRQ
                     RLAAVGKAPPFGRVAGSEPAAARNLIQAIGPALRGLLAAGDNLKVVTFSDAPEVMDLV
                     DSAQGKAVATAGPLTPDQIVYCKSFPLWFEPAAGAPATALVEQLRRAIQQHTRKTRSA
                     PHVVLVKGLGLFAAGDDLTAAETVRQVYTDAIKVMAGARRLGGIRYMNPGLRKFIEDW
                     EVESYRKTVSAAGQAVGRAAGKVIVVTGAAQGFGLEISQDLAAQGASVVLTDVNAAGA
                     VAAAAQINARLGKPRALGLPINVTSGASVAEAIYQIVRVFGGLDVFISNAGVLKAGSV
                     KTLPEKEFDFVTAVNYKGYFLCVQKAAPILAIQHLARKEYTSDIIQINSKSGLQGSNR
                     NGAYAGSKFGGVGLTQSFALELIEDGIKVNAICPGNFFDGPLWSDPQNGLFAQYLRSG
                     KVPGARTIADVKRFYEAKVPMNRGCTTADVVKAIYYLIEQKYETGQALPVTGGQVMLS
                     "
     gene            complement(20300..21589)
                     /locus_tag="PKI07_01305"
     CDS             complement(20300..21589)
                     /locus_tag="PKI07_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49716.1"
                     /translation="MTQRTAEALALLKGLGTPTALAAEAGSGSPRLKRLKVNSHIHLP
                     PNFSAFQTVAQAIELSVAQGIQVLGVSNYYDYEVYGDFAESARQAGIFPLFGLEIICL
                     IDELVQQGVKINDPGNPGKMYICGKGITRLEPFSAEANRLLSLIHHNDSTRMREVIRK
                     LGEVLAQRGLPIPLTEADVIDMVARRHGCPRQRIYLQERHVCQAFQEAIFARVPAGER
                     IEQLVKVLGLATTPKFGPEDAVGIQNEIRTQLIKAGKPAYVPETFINFEQAWRLILEL
                     GGIPAYPTLADGTNPICPFEQPVKKLIANIKARGFTCAEFIPIRNTPEVLTEYARAMR
                     AAGLVVTAGTEHNTLDLLPIEPTCVKGLAVPEELKDLFWEGACVVAAHQFLTLHGECG
                     LVDAQGQPNPNYPNAAARISAFKSLGETVIGRYLNSK"
     gene            complement(21614..23035)
                     /gene="lpdA"
                     /locus_tag="PKI07_01310"
     CDS             complement(21614..23035)
                     /gene="lpdA"
                     /locus_tag="PKI07_01310"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOC49717.1"
                     /translation="MSKHYTIAILGAGPGGYIAALKAAQMGARVVLVEKDHLGGTCLN
                     NGCIPSKAILGSAELMHRIQHAAAMGLEVPAGTVANWGKIQARKDKILQTLRAGIKGL
                     LGARKATVLLGRATLAGAGRVVVSGAAGVLEEFTADKIILAVGSVPSRIPGWPTDTRV
                     VCTSDEALHWQTLPRKLLIVGGGVIGCEFACMMQPLGVEVTMVEMLPQILPNLDGQVA
                     QTLRKILVARGIRIFTGVKIESLALSGAGAVAKLSNGESLETDRVLVATGRRPNTADI
                     GLETIGLTTERGFIRVNDRMETVLKDYYCIGDANGRCLLAHAASAHGVVAVENALGQG
                     HEFKAPIPNAVYTFPEVGTVGLTAEEAQAQGFPVAVGNFPLSFLGKALAVNDPEGFVK
                     VVRNRETGRLLGVHMIGHNATECIAAAGALLHQQVSVQDIAETVFAHPTISEGIKEAA
                     EDALAMGLHLPPRKFLQVTAGVE"
     gene            complement(23064..24590)
                     /locus_tag="PKI07_01315"
     CDS             complement(23064..24590)
                     /locus_tag="PKI07_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOC49718.1"
                     /translation="MPTLTLPPVGKTASKATILRWRKLAEQVVRKGEILLEVETDEGL
                     AVIESAMDGIIKEVLAPAGRTISIGAPLAVIEGVANQSATQPIKQKSIPVTKTTISTG
                     KVIPILMPKAGQSVEEGVIVKWHVQPGAMIKKGEIIFEIETDKATIEVEATDSGRLAR
                     IVAGEGASSPVLQPVAYLADNEADVDAFIAASSTSPASPAAAPAAPVEAAEKPSAELA
                     PPAAAIGTGRIKASPAARKLAAERGVNLATVARGSGPGGRILSTDIPATGTPKPGLAA
                     AEPVAAGKLPAGAIRKRMSGMRKAIARNLTLSKTTIPHWYLRARIDAGPMMAFYKSQK
                     AKVGCSVNDIIVMASARAIMDFPAMHSRVDGESVIEFSGANIGIAVGMDDGLVVPVVM
                     NAEQLTLAQLAVETKRLANQARAGRIENMGQGIFTISNLGMYGVDEFVAIINPPEAAI
                     LAVGAVREDVIVKDGALKAGQTLHLTLSADHRLIDGTLGAQFMARLKALLENPLQLIA
                     "
     gene            24817..25578
                     /locus_tag="PKI07_01320"
     CDS             24817..25578
                     /locus_tag="PKI07_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOC49719.1"
                     /translation="MNSFRQRREKILAAIFEKRHVSARDLAASLPASEATVRRDLKAL
                     AADGEIELVYGGATLRRPSDYSFRSKGERNVEAKRIIGRLAADLVQENEQIFLDSGTT
                     CFEMLPFLKTRRGLSIICSSARLALELDTPGVSVILLGGQYRPDRMDTVGVLASATID
                     QLRGYLCFIGADGLDREFGPAAADLESADLNRRAVRNARQTVLLVDHTKFAAASLCKV
                     VDWKLVSRIVTDRAPSPEWMDFLTAQGIQVDYPGE"
     gene            25645..28122
                     /locus_tag="PKI07_01325"
     CDS             25645..28122
                     /locus_tag="PKI07_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC49720.1"
                     /translation="MPKSLFVNPVENRRPGQIEFTPIPINQYRKTCKEEFAAKRFSKA
                     DFLRIQRDMEIIRAFENMLNEIKLRGSFHGIEYNHRGPAHLSIGQEAAAVGQAYLLGV
                     EDHIYGSHRSHGEILAKGLSAIAKLSDDALLKIMRDYFGGDTLRVVEKDATGSTKDLA
                     IDYLVYGALAEIFAKDYGFNRGLGGSMHAFFPPFGIMPNNAIVGGSGDISVGAALYKH
                     INRKPGIVICNIGDASAGCGPVWEGICFATMDQFKLLWDEAHRGGLPLILNFVNNFYG
                     MGGQPVGETMGFKVLARLGAGVNPEQMHAERVDGYNPLAVIDAIARKKKIIEDKNGPV
                     LLDTITYRFSGHSPSDASSYREKSEVDAWRQQDSLAAFAQELVDAKICTRAEIDEIHA
                     QAEAIILKAFKKAVDIKLSPRSDLKLKDCILERTMFSNLKIESLEPGRKPEVLLTKEE
                     NPRVKQLAQRSRSAFDDKGAKLPKSKCVGIRDAIFEAILDRFYVDPTLVAYGEENRDW
                     GGAFAVYRGLTEALPYHRLFNSPISEGAIVGSAVGYALEGGRPLVELMYCDFLGRAGD
                     EIFNQMAKWQSMSGGLLRMPVVLRVSVGSKYGAQHSQDWTSMCAHIPGLKVVFPATPY
                     DAKGLMNSALVSTDPVVFFESQRIYDEPEHFVKGGVPTGYYEVPFGEPDIKKAGRDLT
                     ILTIGATLYRALEAAKQLEDKWGLSCEVIDARSIVPFNYAKVIESVKKTRKILLASDA
                     CERGSALQTMAAKITQFAFDELDAPPVVAGARNWITPADEVEDAFFPFAVDIIDSVHE
                     HLLPLKGYSVTRDCSNADQLRRYAQGI"
     gene            28140..28835
                     /locus_tag="PKI07_01330"
     CDS             28140..28835
                     /locus_tag="PKI07_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOC49721.1"
                     /translation="MSQFQAIIFDMDGVIVDSEPVHERAYLEVVRELGYGDTHGLRFA
                     DYVGRSDEVLWQDFIARHHPPQSYEELLALKTRRVVEIIRRDQPVFAGVAELIEQLAA
                     RYVLALASGSERQIVREVLGLKGLGRFFSASVAGSEVRHGKPAPDIFLRAAEMIRVPP
                     RACCVIEDSKPGIAAGLAAGMEVIAITNTHPADELRDATHIVRTYSEIQHLLLGPQRR
                     CANRVPDTRTHSG"
     gene            complement(30178..30468)
                     /locus_tag="PKI07_01335"
     CDS             complement(30178..30468)
                     /locus_tag="PKI07_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49722.1"
                     /translation="MTLLQDAMAQLQEGWAASGRARRFESPQSCWARGESARPYAGIA
                     ARLNLTAPVVKMAVPSLRTRCRGIPHAETARTVPSREKIEEELRHLFSAFDT"
     gene            complement(30950..34105)
                     /locus_tag="PKI07_01340"
     CDS             complement(30950..34105)
                     /locus_tag="PKI07_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOC49723.1"
                     /translation="MANRRKGSFGRAGWVYFRRFRMAVWLLILAVLGALIYLNQAGLP
                     GFAKTPLLNNLRARGLDLQFSRLRLSWYRGIVAENVRFVRPDAPLSPQLTLAQVQVRL
                     NLRALARLQIQTDALRLRKGRLVWPVAETHRAPRQLVLDQIQSELRFLPGDEWALDHF
                     TAVFAGAKIRLSGTVTNASAVRHWTAFQAEPAPAGAPRRNWPGEIAEVLERIHFSAPP
                     EFKLDVRGDARVPHSFTIHALLNTPEAHTPWGRLVRGRVNARLFPAATNDLSHAELNL
                     EAGGAQTRWATATNLSLNARLAAFASLTNWGNLELTLCAERADSSWASAAHLTLALQA
                     DAIAGQTNLATTDAVLTVARAASRWGGATNAHLRAQWMHSRADFQPLSGTASLHCAQA
                     SNSWATARELQINARLAMPGHDRPLRADESWGEWAALEPYALDWEGRADTLQSSQLRL
                     REVACAGQWRAPTLALTRIQAALAQQPLGGRGDVDVSTRQARVRFTSSIDPHELSPLL
                     TGAAGDWLARLAWETPPTVAGEASLVWPAWTNRQPDWRAEVLPTLRLQADLNFARGLA
                     CQGVAVSAVQSHISCSNQVWRLPDLRIIRPEGQLEAAVETDLRTREYHGRLHSSVNVL
                     DLRPLLAPDLQRALRFFTFAEPPVIDARVWGRWDEPRRTGLQGRVALTNFTFRGESAS
                     YLQTALQYTNHLLHFTDPRLGRGAERLSADAVTADFAAQKIYLSNGFSTGDPLAIARA
                     IGPHVARVIHPYQFAQPPVARVWGIIPMHGEDDADLHFDLRGGAFTWWRLRASEISGH
                     VHWLGQHLSLSNIWAGCYGGQVHGSAQFDFHPGHDADYQFTVTATNALLPPLVTDLFL
                     ATNHLDGRLNGTLAITRANTADINTWTGSGDLTLRNGLIWDVPIFGLFSQALNSVVPG
                     LGNSRASAGRCTFGITNGVISSEDLEIRSTGMRLQYRGTLDFQGQVNARAEAELLRDA
                     WLVGPLVSTVLWPVTKLFEYKITGTLGAPKGDPVYLVPKVVTLPFLFPFRPLRTLRGL
                     FPENSNANNTNPPPARPPESD"
     gene            complement(34108..35901)
                     /locus_tag="PKI07_01345"
     CDS             complement(34108..35901)
                     /locus_tag="PKI07_01345"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOC49724.1"
                     /translation="MNPRSIWRWAIVAAALMAFIFFHHKYLRETSSGPGPVLPALRPA
                     AVTRVQVRPAARLEICAARTNGSWQLVEPLVYPAQAVSIEKFLAELEQLVPAPYLTAR
                     ALREQPRADEEFGFTTPQASIIIEQPGYTARLRVGARTAPGDQVFLQVVGVEGVYVVD
                     AGFLQFLPRTPDDWRSTALLDLSGMTFERLAVTNGSKIFELRRSATDRLWRMHYPLSA
                     RANNARIEESLQMLQSVRARQFISHDPRADLETFGLHPPELEVVIGQGTNTIAWLQFG
                     KSPTNDAKLVYARRVGLEAIVTVSKDLLAPWYAAVNDFREPYLLHSAVPVAGVEVRAP
                     ESFSLQRQTNGAWRVWPEDFPADADLVNELLTALNELPIVEFTQDVVVAADLPAYGLD
                     SPLRQYTLRAAATNGPAGLTNPVIAELRFGTNQADKVFAQRADESFVYAVPRAEFLRL
                     PTAGWQMRERRIWSLSTNEVARVTLRQPGQLRQMIRNGPYSWSLAPGSPGAMNDLAVE
                     ETVSGLCQLTAAAWLGRGETNRARLGFADNDRQLVLELRNGEKAALDFARETPGQPPA
                     AAVTLNGEPWFFEFPIWLYDYVQRYLFAPSR"
     gene            complement(35910..37397)
                     /locus_tag="PKI07_01350"
     CDS             complement(35910..37397)
                     /locus_tag="PKI07_01350"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOC49725.1"
                     /translation="MATDSSSPPSFTPGRKWGSGLQVLLAGVLVLAVVVMVNYLSANY
                     FLRFHLSARSRIELSARTLGLLKSVTNQVRVILYYDTDDAEALYSPVWDLLNEYRLAN
                     PKIRLETVDYIRNPALAQKTKAKYNLSAQTDKNLVIFDCEGKVKPVDGNALASYVVEQ
                     VTNAVVGANGWSFRRRPTAFLGEMAFTSALLDVTSPRPLTAYFLQGHGEHQMDSGDEV
                     TGYLKFASLLEQNRVRAEPLSLLGTNTVPPDCNLLIIAGPRDAIPDAELVKIEQYLNQ
                     GGRLLALFNFSAARKNTGLETLLAQWGVDVGRNIVTDPDNTAKGTDIIISRFGQHAVV
                     DSLRGSRLHVSLPRSVGRLTARAEAADAPRVDELAFSGPRARGAGDSTHHAPFPLLVA
                     VEKGAIKDLIAERGATRMIVVGDSLFLGNLQIESAANRDFAGNAINWLLERPHLLAGL
                     GPRPITQYSIVMTQAQMRQAQWLLLGVLPGSALLLGGLVWLRRRN"
     gene            complement(37400..38149)
                     /locus_tag="PKI07_01355"
     CDS             complement(37400..38149)
                     /locus_tag="PKI07_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC49726.1"
                     /translation="MLVFRTLVRRELAGFFLSLGGYIIIAAALFLMGYSFIVILAKLQ
                     KPTPMPITELFYITPFFWLILLLTTPVITMRLFAQEKFTGTFETLMTTPVSDGQVVAA
                     KFTAALLFYLVMWLPLLGCLLVVRHQTNDPSALDAGVAASTFLGILLYGGMFLSWGCC
                     ASALTRSQAVAAMISLGFGASVFLLGYLASQLPAQEDWQTQVLGSLALFEQMHDFARG
                     IVDTRAVVLLASATFLFLFLTLRVVESRRWK"
     gene            complement(38149..39108)
                     /locus_tag="PKI07_01360"
     CDS             complement(38149..39108)
                     /locus_tag="PKI07_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC49727.1"
                     /translation="MSDDLMIEVANLTKHYAGNPAVSDISFSVRRGEIVGLLGPNGAG
                     KSTTMRILACYLAATSGTVRVAGLDVFAQSDEVRRRIGYMPENNPLHYDMRVREYLKF
                     RARLKGLSRKHSRARVDVVMEQCGLTEVSRRMIGQLSKGFRQRVGLADALVHEPELII
                     LDEPTIGLDPNQIRVVRQLIKGLGGAHTVLISTHILPEAEMTCHRVVIMYDGRILVAD
                     TPGNLQKLMSSRSQVIAEIAAAPEELRECWAQMAEVEQFDVSPAEGEYFRCALTPRDG
                     VDLRPQIFALARERGWPLRELTRSRHSLEDIYVRVTRPEEEEG"
     gene            complement(39183..40583)
                     /locus_tag="PKI07_01365"
     CDS             complement(39183..40583)
                     /locus_tag="PKI07_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOC49728.1"
                     /translation="MNQQTSKSRRRLGAWWWAGVLCAGSCGVIGQTAPDDVRRDATVQ
                     AVEKAIPCVVNIATETLIRVRDPFEEFFRQFWDPYHRRQPPNSQYSLGSGVVIDEEGY
                     LLTNDHVVRRADKITIKFCTGTNVYTASVVASDPKSDVALLKLNSRPGEKFAAIQFAR
                     EDDLLLGETVVALGNPFGLGGSVSRGILSSKSRVLPKEGVPLDIPNWLQTDAPINVGN
                     SGGPLVNLRGELIGINVAVLNEVMGQRAQGIGFAIPIRRVLESLADIFPTEFVKTYWF
                     GARVKVGTTPLVITSVQPQSPAGRAGLRAGDAIRQVNGRAPRSFIDFADLLAARPGSG
                     VTLAIQRGTERKEVTVRLVPEKTVFNAGMIRDKLGLDLEELTPQLAARYGVGVADAFY
                     IAGVQEDSPAAAAGLQRGILITALDGQLPMDVCAVAKGLYARKKGDRVQMEIAVPQRM
                     GNFNVLRQATVELVAR"
     gene            40704..42176
                     /locus_tag="PKI07_01370"
     CDS             40704..42176
                     /locus_tag="PKI07_01370"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOC49729.1"
                     /translation="MPSHPRKSKGKVLSAQASFYARWRVRCGVVSDLREERDSPPEQL
                     LQSIWQHQRLLREQLKTLDGRPVRILHPGFRSVEGGPDFRGAVVQFGDRPPQSGDVEV
                     DLRPRGWRAHGHDRNPAFQRVLLHVVWEDGPAAPGAPPTLGLRRALDAPLGELSLWLG
                     GEAAPGLPETLRGQCCAPLRQLSPARLTRLLHQAARVRLQSKATQFQARARSAGWEQA
                     LWEGLFRALGYKHNSWPMLRLAELRPRWQTPGAPPFALQARLFGISGLLPAELPRAPA
                     GGSRYVRQIWDFWWRERDAFSDCLLPHTLWRLHGSRPANHPLRRLALASQWLRDDRLA
                     PKLEAWCRREVPAAALPDSLLETLPIEPDEFWSWHCTFRSPRLRRAQPLLGAARMTDL
                     AVNVLLPWLWMRAAEGGNARLQHALEQRYFSWPPAQDNSALRLARQRLLGGATPRALP
                     GAAAQQGLLQMVRDFCDHSNAICDHCQLPKLVQDWMAEDP"
     gene            complement(42809..44263)
                     /locus_tag="PKI07_01375"
     CDS             complement(42809..44263)
                     /locus_tag="PKI07_01375"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOC49730.1"
                     /translation="MNTLLLSHELLVLALGLGLLLVDLWLPLPAKRKLGYVAALGTGL
                     ILLYSLVCVRVPPEGAAGYAFSQMYVLDGLALFFKRFFLLAALLVLLLSVEFADRITS
                     GLSEYYALVLFALAGMLFAASANNFTLLFAALELITITFYILTSFQRHRVASLEAGVK
                     YLVIGALSTAFMVFGIALVYGLSHTLSFDGLARAAGALGGNRVFLFGLVLILAGLGFK
                     IAVVPFQIWVPDVYQGAPVPTTAFLAIGSKAAGFVLLLRVLFLAVPDITAHWPHLLML
                     LSGASILYGNLCAIPQRNLKRLLGYSGIAHAGYLLLGVAALSAAGRAAVLYYLSGYLF
                     TVLAAFTVICVALRQVDGEDLGALAGLHRRSPLLAATMTLAMASFAGIPPLAGFFGKF
                     LLLKAVLEQAPACAAYYYLTFLALAGIVISLYYYFNVVRVIYWSANATDLSPIPVSGP
                     IRLAIYACIAGMLYLGLFPGGILNLATEAVKALP"
     gene            complement(44260..45864)
                     /locus_tag="PKI07_01380"
     CDS             complement(44260..45864)
                     /locus_tag="PKI07_01380"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOC49731.1"
                     /translation="MSPLTYILGWPLGAAIVLLLVPRTFRPVIRGIAILATLMAAVLA
                     VKMFLRFDAAAAGYQFEQQVAWVRSLGLSYHVGVDGINVGLVLMGALVAFAAACVSWE
                     IREREKEFYLLLLVMTGGILGAFAALDLFCFYFFHELALVPTFIMIGVWGRGEQKDYA
                     TFQITLYLSLGALLALVGLIALYVQAGAGTFDIPQLAAHLRAHPLSPGAQGFIFPLLL
                     FGFGILVSLWPFHTWAPPGYSAAPSATAMLHAGVLKKFGLYGLIRIALPLLPDAARDW
                     MPILAWLCLGNLFYGGWVAMRQKDFNRLVGYSSVAHMGFVFLGIAALNLVGITGAVLV
                     MIAHGFLAALTFGLSGYLYQQTGTLEIARLGGLLRRLPFIGAALMMAAFAGCGLPGFA
                     NFAGEITVFFGAWQSYRLVTILACWGALVIGAVYMLRAIRGLLHGPLPEAWAGVADAP
                     HLWRKAPFLLLLASLLIFGCYPRLLTDKIKPAVSEKVAPALAPEIPARPGILARPAPD
                     RDAPQPPDASPAALRRAGAFPRNSRG"
     gene            complement(45867..47729)
                     /gene="nuoL"
                     /locus_tag="PKI07_01385"
     CDS             complement(45867..47729)
                     /gene="nuoL"
                     /locus_tag="PKI07_01385"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOC49732.1"
                     /translation="MHLDFIPWIILFLPLLAAAGITLFTSRHGKLSAALSIGAVSAGF
                     LLSLLYVALTGWDSRPDSAFTWLAIGNLHVEFGLRFDPLSLPMLLVVTGVASAIHIYS
                     WGYMDGDRGYARYFACLSLFTFSMLGIVLANNFLALFICWELVGVSSYLLIGFWFERP
                     AAAEAGKKAFLTNRLGDFGFLLGILTVWAGFGSLNFAQISDGLATGAGQTWCLTGAGL
                     LIFCGAIGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYMLCRVFFIFTPGAL
                     TLIAWLGGFTALLSAVIALQQNDIKRILAYSTLSQLGYMVMAVGLGGPAAAMFHLTTH
                     AFFKALLFLGAGSVILALHHEQDIWRMGGLRQKMPVTFWTFMAGTLALAGVPPFSGFY
                     SKDSILALAAHHNPGLFALGIGVAALTTLYMFRLVFVAFLGSAKSEAAGHARESAPVI
                     AWPLRILALLSVAGGLLGWERLYARQLCAAPAAAPASFLGDLVAPFLHAPVAAGSGLL
                     AVLIGLAAAYGLYARAAQDPLPGMLGALGRAMRNRFYFDELYQATVIRGHDFLARVAD
                     GFDRWILAGVAVRGTHGTTELAGRALRLLQTGNLQTYALLFALGVGLILYLVLF"
     gene            complement(47733..48038)
                     /gene="nuoK"
                     /locus_tag="PKI07_01390"
     CDS             complement(47733..48038)
                     /gene="nuoK"
                     /locus_tag="PKI07_01390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOC49733.1"
                     /translation="MNIGLEHYLAVSMLLFCLGLLGVIARRNLLIIYMSLELMLNAAN
                     LALVACSRFTHNLDGQVFVFFIITVAAAEVAVGLALIVALYRKRQTAHVEDLTLLKL"
     gene            complement(48035..48550)
                     /locus_tag="PKI07_01395"
     CDS             complement(48035..48550)
                     /locus_tag="PKI07_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOC49734.1"
                     /translation="MQDALFYLFAFLTLVFGFLVVANPFSRNPVTSAMFLVLAIISLA
                     GLFVLLHAFFLAAVQVLVYAGAVIVLFLFVIMLLDLKAEEQRKLKAAGVAGGVLAAGA
                     LLAIFLASLWAASRDPAAAGGADAPVLEGGTRALAKLLFTQYLLPFELVSVLLLVGMV
                     GVVLLSKKDLK"
     gene            complement(48629..49165)
                     /locus_tag="PKI07_01400"
     CDS             complement(48629..49165)
                     /locus_tag="PKI07_01400"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOC49735.1"
                     /translation="MIIERQKMSWWERLYLPAIIGGFKVSLRHFFRKKVTMQYPEQRW
                     VVPPGYRGAPYLVRDQDGNTKCVSCQLCEFVCPPKAIKITPPGPAGPPADRPNAEKMP
                     REFEINMLRCIFCGYCQEVCPEEAIFLMKDYSLIGASREEMIYNKEKLLALGGVHQDK
                     IKKWQQKAQEARAQGQKP"
     gene            complement(49166..50272)
                     /locus_tag="PKI07_01405"
     CDS             complement(49166..50272)
                     /locus_tag="PKI07_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOC49736.1"
                     /translation="MIPAILNWAETEAGQLVLFGALKILGVFSVVMFIVAYAVWVERR
                     TAAFIQDRVGPNRVGPLGLLQPVADAIKSFLKEDFTPAHVRKVYYWLAPAIVMIPSLL
                     TVAIIPFGSLLGGQKMVIADLNVGILYTFGIVSLGVYGIVLAGYAARSKYPLIGGLRS
                     SAQLISYEIALGMAVIPLFLIAGGLEAPAASAGWQKLLAGTSSLNLGRLVSWQALHGW
                     FILYAPVSFVICLAAMFAETNRMPFDLPESEQELAGGYNVEYSSMKFALFFMGEYANM
                     VAAAALMTTLFLGGWSLPVAGLNQPAATIGAGLLHIGIFLAKMCFLIFMVIWVRWMWP
                     RFRYDQLMDLGWRRFIPLALANIVVTAAVLWIRS"
     gene            complement(50269..51978)
                     /locus_tag="PKI07_01410"
     CDS             complement(50269..51978)
                     /locus_tag="PKI07_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOC49737.1"
                     /translation="MSTANPTPSPSTPPPADTLQVRVNGKAVEVPRTMPDPVSGKPVP
                     TTMIQACFAAGVMVPHYCYHPKLPVAGNCRMCLVEFGTPALGPDRKPILNPDGAPRIT
                     KSPRPAIACATPVSPGMEIYTDTPAVKQMRESVLEFLLINHPLDCPICDKAGECKLQE
                     YSEDHGEAQSRFVEPKVHKPKRVDLGPRIVLDDERCILCTRCIRFTRDIVGDDALGLV
                     NRGSYSTLTAYPGRPFDNNYTLNTVDICPVGALTSKDFRFQMRVWFLKETPSLCTSCA
                     TGCNIVIGSREDRIYRYEPRENEAVNACWMCDYGRLNYAWIGRADRLTEIRGPQAAGQ
                     APAPGWTPILREISGKLRQAAAGSVAIIASARQTNEELYLLAKLAKKLGALTDSVPRM
                     GEGDRLLLNADRNPNSAGARLTGIAATPMGANLPKIAEGIRAGTIKTLLVVGEEVTRL
                     GLGADLLARLETLIVSDILPNETTRRAHYLLPGCAHAEKRGTFTNARGRVQKFMKAVE
                     PRGDARPEWEFLHELALQVAGPDGCQSLEGLFNQMARDLPAFAGLTWAGLGDQGATLR
                     SEP"
     gene            complement(51981..>52816)
                     /locus_tag="PKI07_01415"
     CDS             complement(51981..>52816)
                     /locus_tag="PKI07_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOC49738.1"
                     /translation="AVLGLYQCPTIVNNVETLCAVKHIVARGGAEYARLGTPNNTGTR
                     IVSISGHVRRPGYYEIEVGKVTLGELIHDPAFGGGLREGRQLKAIIPGGSSAKVFKAG
                     ETFKLKRRGPDGKESVQEVALLDLPYDFDSLIAAGSMSGSGAIIVLDDSTDIVAALAN
                     IADFYAHESCGQCTPCREGSLWMAKALRRLTHGEGRPEDADYLAHIADNIPGGRTICA
                     FGEACAWPVQSFVAKFRDEFIARGAADAKRRAASGRETAAPSGPPHPATAAALAQTPA
                     E"
BASE COUNT        10209 a        16349 c        16920 g         9338 t
ORIGIN      
        1 cggggcggaa cgggaggttg aactccaagc tgcgaaaaaa ccgccagttg tgaatctttg
       61 gcggatatga gcgagatttc cgagggaata agatcgcccg gaaatcgccc ggcttaataa
      121 gtggctgatc cgaagcgact tagctggagg cttccatcgc acgccatcgt tctatcgaaa
      181 tccattccac cgctccctgt atgtgtaaac cattgatttt atagtcggaa ggtgtttggg
      241 atttaatgtt tatgccgttg aatttgctaa aaaagagggt tgccggagcc acggtctggg
      301 cggcctgggc tgatcaggct ctgatggaac ggaggcaggc agttgagcat ttctcctgaa
      361 tcgttccggc taaaggtcct tgaagctggc aaaaaccatg cgtgaccctt gccgggctga
      421 gcgagtgggg gttaaccgcc gcagaatcag ggaaaaatct tcggggcacc gtcccgtcca
      481 gccctaatac agcatgtccc aagccggggc aaagccagcc gggaggaaac cgtctcgcag
      541 gtcgtcagag ttgggccgcg gcgggcctct ggaaaacgcc ttctgtcagt tcaaaagcgc
      601 gcccccaccg tcagcattac ggcgtggctg aggaaatcgt aagtgccatc cgcccgttcg
      661 ccggagctca aggcgggctg cgaggagggt ttgctgccgg tgaccttgtg gctcgggccg
      721 tagccgaatt gataggccag gtcgaaattg tagcgctttc cccggaaccc gccgccgatg
      781 ctgaaaaagt gccggtccag gtccgccgcc agcggggtgt aataggcgtt cggcacggag
      841 ttctcgctaa aggcgtaacc ggcgctcaga tgccagccgg gctcgaagta tcgagtcacg
      901 ccaaaggagt aaatccaact ggattgccag tagagtttga catccacgtt tctggaaatg
      961 ggatacggcg gggcgggcga ttggtagagc gtggttttgc caaagctgct ccactccgtg
     1021 taatccgcgt taaactccag gttccatttc ggggtgggcc ggtaggaaat ccccaccacg
     1081 gccgttaagg ggaagtccag ttccatgtgc gccgagcgcc gtccctgctt gatgttcggc
     1141 tggcgcaaaa agtccgtatt tccctccagt ttcaccgggg ctgcaccgcg aaacgttgcg
     1201 cccagcgaga gtttcctatg cggctggaaa agcacgccca ggttgtaact ctcgctccag
     1261 ccatccccct taaagcggaa gtaatccttc aaaagcgggc tgagcttgcg cagcccctgc
     1321 tccatttcca ggtccacgta attgatcatc aggccgccgc ccagcgacag ccaggacgcc
     1381 ggccggaacg ccaccaccgg gttgatggtc gcgtaagtca gcgaggcgtc cgtggcgatc
     1441 gagcggaaac ccgtgttctg cggccagctt atgctgccgc catacggcgc ataaagcccc
     1501 acgccaaagc tcagcggcca atcccggggc gtgtaagtgg caaacccctg gggaaccgcc
     1561 gcccaggaat ccttgatatg atacgtattg gtgcggcctg gcccgggcgg cgtaaagcgc
     1621 ggctcgtagt aaagcccgct gattccgccc cggaagttga gcccctcaag ctgggtaatg
     1681 cccgccggat tgtagaaaac ggccgaggca ttgtcggcgg tggcaacgaa cgcctccccg
     1741 cgcgccgtcg caaaggcatc ctggcctccc agccggaagc cgttggccgg ggcctccaag
     1801 gcccaggccc cccacagcaa aaccgtcagc aaaccggaaa tcgtccgccg gttattgatc
     1861 atagtcagtc cagtcaaatt agcacgccgc ctcacctaaa cacggaacgc ccccccagac
     1921 aagcaggttt cctgccgcgc tggccggaaa gcctgccact cagagccaat cccgctcgcc
     1981 gcactccttt ggattgccat cgctgcgctc ccttgccccg gggcttgcgg ccccgcgggc
     2041 gcttcgcggc ctgcagactg cgccgctggc aaggcggcgg ggcagttttt cggagccgcg
     2101 ctcggctttc tggctgcctt atgcttgccg ctggcgggac aaggcggtac gctggagccg
     2161 cataaagcaa aacatgcggg cgtgagtggc atcgagagaa agagtgactc gacgaatgcg
     2221 gcggatgcct attgcaacgg gccaacaggg caggttccat acaaggaact ggccgaggtg
     2281 ttcaaggctt cgagggtgcg cgttcggggc ggggacgcga gtgagcccgg cgcgggatga
     2341 tctgcgcgca cggggttgcg ccaccatgcg gagtatcttt cggatgggga atcgcggttc
     2401 gcgcgcccgc tcgcgtcagg cgctgcgtgg gcgaagtggg cgcagcgggt ctggatgcta
     2461 tcgtaaggca aacaccgcca aacttcgggc aaccaacgaa atcctatgag cgcagagaac
     2521 ccggcagcca gcgagaacac aaaacagacc accgcctgca gtcggcgcga cgccctcaaa
     2581 ttcctggggg cggccaccgc cggactggcc gtggcggcag cgccgccggc aatggccgcc
     2641 gccggccccg cgaaaggggc gaatcccgat ggcggcgccc ctgggagagg catcagtcgt
     2701 gaaacgctga gctcacgcgg gagcgcacaa ggcggcatcc tgcccgtcaa atacgaggtg
     2761 gcggtctgcg gcggaggccc ggccggcctg atggccgcga ttgccgccgc gcgaaacgga
     2821 gccaaaacca ttctgatcga gcggtatggc tttctgggcg ggatggccac gcagtcattg
     2881 gtcagcccgt taagcgtatt taacaaaaac cggaaaagga ttatcggcgg gattccgctg
     2941 gagtttgccg aactgatgga gacgctcgga ggcgcggaca ccacttaccc gtccgggaac
     3001 attcactttg atccggagat ttacaagctt gccgcccaac gaatggtctt tgcatccggc
     3061 gggtcgctga ttttgcattc ctatatttcc ggatgtctca gggaggggga tggaaatggc
     3121 agaatcacgg gcattgtgat cgagaataaa tcaggccggc agaaaatcga agcccggtat
     3181 ttcattgact gcaccggcga tgcggacgtg ataaagtttg cgggtcttcc gtatgtgatg
     3241 ggggacggcg aaagccatga actgcagccc atgacgctgt ggttcaggct cggcggagtg
     3301 gatacggacc ggcttgagaa catggagatg cgagaaagcg gcaagcgcca cgtcaattcc
     3361 cgcatccggg acgcgctcct caagctgggg aacgaggccc cggtgcctca atttggcggc
     3421 ccgtggatgt tcacaacttt ccgaaaagga gaagcctccg taaatatgtc gcgctatgcc
     3481 gggaacggaa ccgacgtgct cagcctgacc caggcggaat gcactttgcg cgaggatgtc
     3541 ttcaaactgg tgaagctgtt gcgggagcac ttcccggaat tcaaaaacgc ctaccttatt
     3601 gacaccggga cgcaggtggg aatccgggaa acccggagaa tcaccggctt atacgagatg
     3661 accctggatg acatcatggc cccgaaggat ttcccggata ccgtggccaa aggcggccat
     3721 cctgtggata tacacaaagc ggggagccgg gagcaggatc tcagggcagt caaaaccggc
     3781 tacaatatac cttaccgatc catgattcct gtgggatcgg acaacttaat tgccgccgga
     3841 aggccgatct gcgcggcccg ggaagcgttt gcctcaatcc gggtgcaggc tacctgtatg
     3901 gccctggggc aggcggcggg aacggcggcc gcgttgtgcc atcaaaagga tattccggtc
     3961 cataagctgg acggcgcgga actcaacggc attctgcgca agcaaggcgc ggtggtataa
     4021 aaccggagcc acagccgcac cctccaccgg ctggccctgc cggcatactg ttgccccgat
     4081 ccgccccttc cgccaatcat cgaggaactt aaccgccgcc caacccgcag cctcaacctc
     4141 cctggctcat gatccgcaaa gcaaagggcc cggcgccaac aggcaagctg tcgaggcccg
     4201 cgcccgcaat cgaaggcggg ttgcgtcagg aactggcgct ggtatagcgg cgcatggaaa
     4261 agcgccagcc cagctcggat acggcgaagc aaaccgcgct catgcccagc aaaaggagca
     4321 cggggccagg tgaactgagc ttgtcggcca gcaggcggac gggcacgttg gaaacgagca
     4381 gcatcggcag ggcgaaagtg aacagggctt taaacaggcc gccgaaggct tcgtcaggca
     4441 ggcgggccac gttgaagaga ttgtaataac cccagacaat gccctgggcg cgcacggtcc
     4501 agaagctgat gctggccaac agaaacatca gtgagtagtg aatcatcacg cccgcggcgc
     4561 agagcgccag gaagcctgcg atctgcccgg ctgagggcgc cagctgcaac tggcgcgcag
     4621 cgtagatcat cacggcgatg gcagaagcgg cgttgacgaa cgcgcccagg tccacttgcc
     4681 ggagcgacac aacaaagcgt gtgttgaccg gcagcagcag cagaaaatcg agtttgccgt
     4741 tgcgcaccag ctccgagaga ttggtgcagt taatcaggaa gaaggcctgg aaaatctgtt
     4801 ggacgaagtg gctggcgcca atcagcagca cgacctgcca ctgggtccag gtgccgatgt
     4861 gttccgtgtg caggtagagc acgccgataa agcaaagttg catggcgaac caaagcagct
     4921 ccaccagaat ccacagcagg aagttggcct tgaaactcat ctcgcgcgca accgagtttt
     4981 tccagagcgc ggcatagact ccgcaatacc ggagcgccgg gcggaaaagc cgggaaaagg
     5041 agggggaggc gggagggggc gggcgcgtgc cgacgccgct tctttcgggt tggggattgg
     5101 gactggtggg tttcatttca tcctcctacc gcggagtaat gcttgatccc gcgccgccat
     5161 gcgaaccgcg ccgcgccgta cgccgcgatt acccaggaag cctgcaccag caacccgcga
     5221 agaaggtcca tgcccgccgc tttgccgata taaacactca ccgggaaata gagttggtag
     5281 ggaaacgggg tgaaatagag cgcctgctcc aggccgcgcg gcaggatgtc cagggggaaa
     5341 aggtgtccgc tggcaatata ttcgaacgcg tacaggatga aaataaacgt ggagacctcc
     5401 agcacccaga aggccagcat ggccatcgcg taagacatga aaaactgcag cagggccgtc
     5461 aacgccgtgg aaaggaggaa ggccgcgagc gcaactccgt ccgccggcgg ctccaggtac
     5521 gaacggaagc acgcaaaaaa gagcgccagc gggatgaacg ccacggccag gtaggtcagc
     5581 cggcccgaca ggtacaggca tagccggtac caaaggtaat caatcggttt aagcaggagt
     5641 tggctgatgt tgccgtcctt gatatccgcc gcgattaacc aatcgtcctc gttcaccgcc
     5701 gtcagcgcat ccaccacggt tatgagcagg taatacgaga tcattccagc cagggaatag
     5761 ctgccgacgc tggttccggc gcccttgccc gcgtaaatct tctgccacac gtataacatg
     5821 gcgatgagcg ggaccaggcc aaacaacgcg cgggccagga agttgaatcg gtacgtcagg
     5881 ctgttctgaa tcccaatatt tatgacgtgc cagtattttt tcatccacgc gcagcaggag
     5941 cgtatcgcag ggggagccga ttgggaagcg ccgattcagg ctggcctttt ttctccggca
     6001 agtcatgatt tgcctcatcg ccggataccg taaaactatg atcacgcaga agcgctgccc
     6061 gttttgccaa acccccgccg ttatgggatg cccacacctt gcgattgcgg tggaaggccg
     6121 cgattttgtc cgccgctgcg tggaactgtg ccaggggcag aaacaatggc gcgccctggc
     6181 cgagctgcgc cagcggcagc ggcaggcgac gggcgagtgg accccggaac gcgacgacta
     6241 tacctggctg gaaacggctt tctgtgagga gtttttgaag ccgctctcct ggtttggggg
     6301 cctggaacac gaatggcgga tcgggccgaa gggcaatcag gccggctttc tcgtgctgtt
     6361 gtggtcgaaa gacccgcgcc ggctctggtg gcagttgcgc gatgaacttg agcggcaggc
     6421 gcaggaaccc ttcccgggca aacgccggaa ggttaagggc caaagcccgc gggctggccg
     6481 gcgggatggc gatttgcgcc tgctttgacg agggagttgc cgccgcggaa aagacgacta
     6541 cactcgccgc atgattcgga acatcatctt cgattggtcg ggcacgctgg tggacgatct
     6601 gccggcggtc tggcaggcga cgaactatgt cctggcgcag gcgcggcagc cggagatgag
     6661 cctggaacag ttccgcgccg agttttgcct gccgttcaca attttctacg atcgccatgt
     6721 gccgcacatc ccgatggcgc agctggagga atggtttcac tgccagtttc ggcaggtgca
     6781 ggcttcggtg cgcgcgctgc cgcatgcgcg ggaatttctg gagttttgcc gcgcgcgcca
     6841 gtttcgcacc ttcctcctta gcaccgttca ccacgagtat tttgacattc aatcagcggc
     6901 taccggcttt gggccttttc tcgacaaacc ctacctcaat gtctgggaca agcgtcagaa
     6961 aatacacgaa atccttgcgg agaaccaact ggcggccgaa gaaacactct tcgtgggaga
     7021 catgcagcac gacatcgaga cggcgcggca tggcggcatt cattcctgcg ccgtgcttac
     7081 gggctacaac actctcgatc aattgcggca ggcggagccg gacttgattg tcgagcatct
     7141 gggtgaattg cgcgaagccc tggaaagaaa cgggttcagc ctcaaagcct ccccccggca
     7201 ggtttcggaa gaccgcctgc cggtcgccac cgtgggcggg ttgatcttta acgcccgggg
     7261 cgaggtgctg atgatccgca cgcacaaatg gtctgatctt tggggcatcc ccggcggcaa
     7321 gattaaatgg ggagagacgg ccatcgaggc gctgcgccgc gaactgcggg aagaaaccgg
     7381 cctcgaggtt tccgacatcc gatttgtgct catgcaggag tgcattcact caaaggaatt
     7441 ttatcgcgcg gcgcacttta ttctgctgaa ttatacctgt cgttgcgtgg gtgagcctca
     7501 ggtgcgcttg aacgaagagg cgcgcgaatg gcgctgggtg actccggcgc aggccagcga
     7561 aatgcccctc aatcaaccca cgcgcacgtt gctgtcagcc gccacccggc tcgagtccca
     7621 aacaaaaaaa catggctaag ataacgattg ttgacttgga agtgtattat tgcgtcgggg
     7681 tgaccgacga agaacgggcc cagccccagc gcctgctgct gacggtggat atgaactttg
     7741 atttttcttc cgccgcggtg agcgaccgca tcgaacggac gatcaactac caaaccgtgg
     7801 cggaagacct gctgcgtttt gggcagggcc ggagctggaa gctggtcgaa aagctggcag
     7861 acaatatcgc cgaacgcgtg ctcgcagaat acaaacccca gggggtgctg gtggaagtaa
     7921 agaagttttc catcccgcaa gcacggtttg tctcggtgag cctgggcaag acgcgcccct
     7981 cacgctaaac ccgccggagc gcactctccg cgggatgaat cgccggtcaa ccgggtaaac
     8041 cggtgatgta ggtttgcaac tggctaatgg tggaatgctg tgcggaaatg atccagttga
     8101 ccaggtcgcc gatcgaaact atgccgacga ttttatctcc ctcaaggacg ggcaaatgcc
     8161 ggatatggcg atcggtcatc aagcgcaggc attcctcgac cgtatgcgtt gggctgacat
     8221 agataacctg gctggaaagg atttcccgca ccgcggtcgt tttggaggat ttgcctcgga
     8281 gcaccacctt gcgtgtataa tcccgctccg aaatgatgcc gactagtttg ccgccttccg
     8341 tcaccagcac ggcgccgaca ttcttgtccg ccatcagttg gatcgcctcg aagacggtgg
     8401 cttccggggc gatggcccag gcagccctgc ctttcagact caggatgtcg cttactattc
     8461 cggttggttt catcactgcc gcaatttatc acggctcctc acaaatgaca ctaacattct
     8521 gcgccgttca ctgacagaat tgctcataga acgtcacgac ggaaatgatg aagccgataa
     8581 gcgtgatgat tagccgaacc tgttgtgggg gaatgcgctg agcataatgc gcgcccagaa
     8641 aataaccaaa cagcgccccg gcggtcatca cgccggcctt cggccagtgg atcaaccccg
     8701 caccgataaa ccatccagcg gcgaccagat tgatgagcga ggcgagaatg gtcttcagcg
     8761 tgttcatctc gtggatatgg ctcaggccca tgaaacccag tgaagcaagc atcaggatgc
     8821 cgatgccagc cccgaagtac cccccgtaaa ccgcgaccgc aaactgaaaa aagaccgccc
     8881 cccaaaccgc gcgatgtttc cccttggagc tggtgtggac gcccgggctg ccaaaccggc
     8941 ggaagacgcc ttgggccagg aacagcaaag tcgcaaacag aatcaggaac ggcacgagtt
     9001 ttgagaagac ccggttgctg gtgcaggtca acaggatggc tcccagcact ccccccagca
     9061 cgctgacggg cgcgaagcgc catagccact gccggacggg tccgaggtgc tcccgatacc
     9121 cgtaaatgcc gcccgaagtg cctgcgacca gcgccagcgt gctcgtagca ttggcgatca
     9181 ccggcggagt gccaaacaat agcaataccg gaaatgtgat taatgtcccg ccccccgcaa
     9241 cggcattgac cgctccgccc gccatcgcgg caatgaccaa acccaggctt tccgtcacca
     9301 tcatcgttga cgggcgaaga tagagagagg tggtgttaac acaagcccaa cctgctgagc
     9361 ggaaaccttt tatcagatgg tgccgatgga gggggtcgaa cccacacact ctcacgagta
     9421 ccagattttg agtctagcgc gtctgccaat tccgccacat cggctaccaa aaacaggctg
     9481 aatatgaggc cgtcagggcc aattgtaaag cagaagagtg cttgagaaac cgcgaaggtt
     9541 ctacgatggt ttccgcgtgt atctatctga cccatttgat gatctgctca tttgccggct
     9601 gagtgagggg cgcaaggcgg tggaaccgtg cgtcattatt atctttggcg cgagcgggga
     9661 cctgactgcc cgcaagcttg tgcctgcgct ctatcacctg tttaaagaaa agcaaatgcc
     9721 cccagccttc agcgtggttg gcgtggcgcg gcgtgaaaag acggatgcgt catggcgcac
     9781 agagttgcgg tccgcgctgg atcggttttc tcgcactcaa ccggtggacg aagcggtctg
     9841 gcaggatttc gcgcagcatt tgcagtactg ccagggggat ttgaccgccg cggcaactta
     9901 cgaggcgttg cggaagcgac tggccgctgc ggagaacgag ttgctgcgcc gcaacctgct
     9961 cctttatctg gcgacctcac ccagccagtt tggtgaagtt gtcgagcagg tatatcgcgc
    10021 cggattgttg cacaaagaag gcggcgcagg ctggcaacgg gtggttgtcg aaaaaccttt
    10081 cgggcatgat ctgccctcgg cccggcagtt gaaccaggag ttaacccgct ttgccgggga
    10141 acaccagatt ttccgcattg atcactacct gggcaaggag acggtgcaga atatcatgat
    10201 gttccgtttt tcgaatgcgg tgttcgaacg gttgtggaat cgggaatcca tagatcactt
    10261 tcaaatcacg gtcagcgaga agctgggagt cggccagcgg ggaggctatt acgaggaggc
    10321 gggcgcattg cgcgacatgg tgcagaacca cctgctgcaa gtgttgtcgc tggtggccat
    10381 ggaaccgccg gtctcactcg cagccgaagc catccgcgat gaaaaggtca agctgctcaa
    10441 atcaatccgc cccctgagcg agggagacat ggcgcgccag gtggtgcggg gtcaatatag
    10501 cgcgggaatc gtagccggag aacaacgcct gggatatcgc caggaggcga aggttaatcc
    10561 gaactcgaat gtcgagacct acgtggcgtt gaagctgttt attgataact ggcgctggtc
    10621 aggcgtgccg ttttatctgc gcacgggcaa aaacctgccg ctgagcacca gcgaagtgcg
    10681 cgtgcagttt cggcgaacgc cccatgttct attcgcggcg caatgcgggc agaagcttga
    10741 ccccaatgcg ttgacgcttc acctgcaacc gcacgagggc atctcgctcc ggttcaatgg
    10801 caaggtgccc gggacgagcg tggcggtgcg gccggtgcgc atgcacttca gttacgacgc
    10861 cgagttcggg gcctacacgc ccgaggccta cgaacgcctg ttgctcgaag cgatggcggg
    10921 cgacgcgacg ctcttcattc ggcgcgatga agtggaggcg gcatggcaat tcgtggatga
    10981 cattcggcgc gcgtgggaac acaagccact gacggatcgg gagttttacg cggcaggcac
    11041 gtgggggccg gtggcggcgg aagatttgtt ggcgcaaaac ggccatgcct ggcgcgagcc
    11101 gctggtggtc agagtttgat ttgccgactg cggcgcgcgt ggactttggg cttttaagcc
    11161 cgccgttttg gcgtaggttg cctttatgac attaactgag cagatggtcc agttggcgcg
    11221 gcaggcgaaa accgcggcgc gcgaactggc ccggttgacc cccgacgata agaacgcctg
    11281 cctgcttgcc atggccggcg cgctcgaaca gaacaaggcg gacatcaaaa atgccaacgc
    11341 ccgcgatatg gaggtcgggg cggggatggg cctttcaccg gccatgctcg accggttgaa
    11401 actcaacgac cggcgcatca tcagcatggc taacggtttg cgggaagtcg cggcgctgcc
    11461 cgaccccatc ggcaaggtgc tcgacgaacg ggtgcgtccc aacggcctga agctccgcaa
    11521 gatcagcacc ccgattggag tggtggtcat catttacgaa tcacgcccca atgtaacggc
    11581 cgacgctgcc agcttgtgtt ttaaatcggg caatgcaacg attttgcgcg gcggcaagga
    11641 ggcgttgaac tcgaatcaag ccattgcgcg aattatgatc gaagccggca agcgggcctt
    11701 gcggggtttt cccgagtgcg ccattcaagt ggtgcccact cccgaacgcg cagcgattaa
    11761 ggaattactg tctttgacgc aatacgtgga cttatgcatg ccgcgcggcg gggagggctt
    11821 gatccgcgcc gttgccgagt gttctaaagt gcctgtgatt aagcactaca aaggcgtctg
    11881 ccacgtctat gtggatggtg cggctgactt aaagatggcc gaagcaatcg ccctcaacgc
    11941 caagattcag cgtcccgcgg tatgcaacgc aatggagacc ctgctggtgg atcgcaaagt
    12001 ggcgggcgta ttcctcccgg tggtggcaca tcggctgggg gaaaaaaagg ttcaattgcg
    12061 tgtggacgcc gcggccggaa tgatactgaa atccgaaagc gaaaatccaa cattcgagat
    12121 tagagccgcc acggagcagg actggtttaa cgagtataac gactacatcc ttaacgtgcg
    12181 ggtggtggac ggcgtgcggc aggcgataga ccacatcaat cactacggct cggctcattc
    12241 cgacagcatc gtaacgcagg atgaaacctg tgcgcagcag ttcctcgccg aggtggattc
    12301 cgccacggtc tattggaatg cctcgacgcg tttcaccgac ggcggtgagt tcggcatggg
    12361 ggccgaaatc gggatcagta ccgacaaagt cggcgcccgg ggaccaatgg gattggacga
    12421 attgaccagc tacaagtgga ttggcattgg ctccggccag gtgcggacct agagcccggc
    12481 attgctcgcc gacgcgcgag atgctaacga cgcatgaact cagctcaggc aaacgctcct
    12541 ccgaccgagg ccgctgaacg cgctcgtttt gtctatgagc acctgcccgc cgaagggctg
    12601 ttcgcgggcc agaactggcg aatttctccc ttcccctgtc ctttaggagt tgcgctggtc
    12661 agggaactgg agacgcttgg gcgcgttctg ctgcagttct accgcgcggc caacctgttg
    12721 taccgccgga gcgtggaagg tcgtcaaccc gcttgggtcg cccggtggct cgacctcggc
    12781 aagccatctg cactcatcca acttcaacga tccccggcgc tgaaacaggc gctgccacgc
    12841 gttatccggc cggatttgct gattagcgat cacggtttga gcgtgacgga actggattcc
    12901 gtgcccggtg gcatcggcct caccggctgg ctcaaccaaa cgtactccgc cacgggcgca
    12961 gaagtgcttg gcggccccga cgggatgctg cgtggctttg ccggcatttt tggcgacgcc
    13021 cccaacgtgc atatcgtcat ctcggaagaa gcggccacct atcgtcccga aatggcctgg
    13081 ctggctgagc aattaggcgc atcccgattc caggtccgcg acgcgcagtt tagcgcattc
    13141 gccgacggcg acgccgtcta tcgatttttc gaattgttcg atctggccaa tgtgccgagc
    13201 gcgaaagacc tctttgcccg ggcggcggcc aggcgggttc acctgactcc tccccccaaa
    13261 ccgctctttg aagaaaagat gcttttcgcg ttgctgtgga accgaaactt gcgcggtttc
    13321 tggcggcagg aactggggga gaacttctac caacgcctgc tgcggctggt tccttacacc
    13381 tggctggttg accccactcc gctgcccccc cacgccgcta ttccggaatt gaacctttcc
    13441 gactggcatc agctcaagga actctcccaa aaagagcgcg gcctgatcct caaagtttcc
    13501 gggttctccc cccgcgcctg gggcgcgcgc ggggtctttc ttggcagcga cctggcgcag
    13561 gcggattggg ccatcgtagt ggacgaggcc cttgcgaatt ttgcccggtc gccatgcgtg
    13621 ctccagcgtt atcacaagcc caggctgatc caggcgcaat ggttcgattt cacgcgccag
    13681 caactcgtgc cgatgcaggg gcggctgcgg ctttgcccct attattttgt tacgggcgat
    13741 gccgatgccg cgcgtgttga aatgggaggc gtgctggcga cgatttgtcc cgccaacaaa
    13801 aagatcattc acggtatgaa ggaagcgatc ctcgcccctt gttcgctatg agcctgtttg
    13861 aatatatttt cctggccgcc agctcgcttt ttgtcattgt ggacccattt gcggcggtgc
    13921 cggcgtttct cgccatgacg ccccatgatt cagccgagca acgtattaaa acggcgcggc
    13981 tggccagttg cgtatccgca ggggttctgt tggcctttgc catcgccggc aaatggattt
    14041 ttaagttcct cggcatcacc atgcccgctt tccagttcgc cgccagcatt gtgctgttgc
    14101 tggtggccct ggacatgttg cgcgcgcagc gttcgcgcgt gcaggagacc ggcgaggaaa
    14161 cggcggctgg cctggagaaa gccgatatcg ccgtgacgcc gctggccatc cccatgttgg
    14221 ccgggcccgg cgctatttcc acggctattc tgctccataa ccaggcagcc aacctcggcc
    14281 aatgcgcggc cctctatgtc tgcatcgcgg tcgtatgctt tgcgagctat tgggtgctgc
    14341 gcttttccgc gcgcagcacc cgctggttta gccccattgc gatgcgcatc accacccgca
    14401 tcatggggct gctcctggct gcggtagcaa tgcagttcat gcttaatgcc gtccggcaat
    14461 tgaacccagc gtggtttccg ccggcagctt gatcttagcc aaacccatgt cgctgggagc
    14521 agcgagagcg catgcctcga cgcgactgat ggtgcttcgc tgccggttca tagaggttct
    14581 gggtttggat cctggccaag cggacttgcg ccagtggact gaggttttca gggtttgaac
    14641 tcttcggtgt ggtcattgta gagcacgcgc ccgccatcct ggggacagcg agccgattcc
    14701 caaaacgagg gccgctccgg tatctccagc aacaccatgc cgctggcagg ctcaaagcag
    14761 ctcgtggtca cttcccagtt taatcccaat cccaatagtt catgaaataa ggcctcgggc
    14821 gccatccccc aagcctgctc ctccttctgg cctattccgc cgaaatcgtg gaggaaccgt
    14881 cattcccaac ctaacctccg ccatttcaag ctattccata gctaccgcct ggccgtgtac
    14941 aaggggtgta caacgggtgt acaacggatg ctcaccagat gtactatgga aaatcctttg
    15001 tacacccgtt gagcatccgt tgtacaccac ttgtacacgg cgtaggggca aggtatgtcc
    15061 gggctgcgtt cggggtaagc cgaaatcggc atgattctgc ctctatttta ttcatcttca
    15121 cacctaaact attgatttta caggcgaaag gctcgggatt cagcagccac gcccttgaat
    15181 tggttaaaca cagagttcgc cagggctgca gtttgggcgg tcaggcccgg tcagactcca
    15241 atgaaacgag ggtgggcggt tgaattaccc aactgaagcg ttccaaccaa ggcagcgagc
    15301 agccgggatt gggggtgagg gacgtcttta agccatcccg gattcccagg tcaggtctat
    15361 gagggagggg gcttccgggc cggctttggt ggaccagatg gcacggagca gattgccgac
    15421 cgattccttt gaccacgatg cggttggccg cggcagtgcg gccacggcct ggccgaggcc
    15481 atcccattgg tctatcagag aacggaccgc cgccgccgcc ccttgcgcga ggtgaacggg
    15541 gcgaatgcca tgccgcaggg ctaaccgcat ggtgccgaaa aagcggtcat cgtagccgag
    15601 tttgcgaacc tggtcacggg tgacgcgggc gatgagatcg ttgagggtgg ggcgaaccat
    15661 gcgcgccagc aggtcgtcgg cataggcagc gtaccctgcg ggagtaaaaa ggggatctcc
    15721 cagcgcggcg tgtttgcgga tcagggcggc ccccgattcg ttgataaagg cggcccgcgc
    15781 ggtcgccata atgtcgagat gcgcggccgc atcggacatg gccgtcaacc cgcgccgggc
    15841 ggccagataa gcgatgagcg cgtggatagc gttatggccg tagagtttgg cttcttcaaa
    15901 aggcagcaag tcgggctttt cgatgaatac atcaattcca cgggtgaaac cgggcagggt
    15961 gatttgcgag atcaggatat ggttgaactc ctcgaccagc acggcttggg gaaccgccgg
    16021 ggtcagcggc gtcagcttca gccgcgccag ggtggcgggg tcggtgatga cgccggacat
    16081 tttgccaatg accgtgttga ggaactggat gccctggagc gttggcgggg ggcagtattt
    16141 ttcgacggcc tggcggagca gctcggcagc gtgattgtgg ttctcggccg catagatgac
    16201 ggtggggcgc ggcgtgggcc ggagcgcgaa accgtccgcc agcgcgcggg ccacgctggt
    16261 cgggtggtcc cggtcgaaga aggcgacgct gggcagcgag gtggctagtt cgtcactttc
    16321 ggcgatggct tgaacgattt ggcggcgatc ttccgggacg gcggggttgt agagttcgac
    16381 gccggaaacg gttgccggtt cgatgcggtc aggaagcgca atgttgatgg tgtagcgccc
    16441 ggcggcggag cgaaccgcct ggaccatggc ctcgtttacc tcggcgacga cgaggcgcga
    16501 gaaatgcccg gagcgaaacg cttcatagag aaagagcgcg ctctggattg gcccgaagcc
    16561 gaagccgacg aaagttcggg ctgccatcgg ctaggctttg gcggccacgc gaagcaactc
    16621 gtcctcggcg gcccgggtcc actggccgtg ttcgagtttg gcggcgacag tgggatagtg
    16681 ccgggagagg tccgccagcg ggataagccc tacatcatag agcatcgggt aaacgacgat
    16741 cttgccggcc aaggtgcgat tttcgacggc ggcaatgccg gcgcgggctc cggccatgcc
    16801 gctgatggcg tccaccgagc aattcgtgtt gagttgcccc gaggtgacct ttcgcagcac
    16861 gattttcatg tcctcgatag tcgagccgct ggtgccgaac ataaagcagc gattggcaat
    16921 ataggtatcc aaatccagct catgtttggt gggcgcgggg atgccggcaa agaggttgat
    16981 gaggcagccg ggctggctgt cgcggatgct gttggcgacc agcgcgggca ccggggccat
    17041 gagggcaaaa taagaatatt tgcccgggag gggtgtttgg gccgtgttca ccattttcag
    17101 ggtcacgccg ttggcccggg cgaacggctc cgctttttgc cgcagcgaag ccagccgggc
    17161 gtcatccatg tcagtgccaa tgacagtgag gtgcggcacc ccggaacaga gggcgcgaat
    17221 gacgtgcatt tggcccatgg ggccgccggc tccgatcacg acgaagtttt cgccgctgcg
    17281 aagttcgcag gtgcgcggaa tcacctggta ggactgggcc gggtcgctgc cagtcgtgcc
    17341 gatccagcgg gtcatgccat agtgaattcg gccgacgccg atgttgacgg gggccccgat
    17401 gcgccggccg ccgagcacga tattgatgat gccgcgggcc gcgagcttat cattgagaac
    17461 ttccaagacc gctttgctgg cgccgaagta gaggatgtcg tcaaaggatt gatcagccag
    17521 agtcccaacc tgggccgggg tgtggcgcaa gacttcggcg ggcggaccgg cgggggcgtg
    17581 cgggaggccg tccagtgaaa ccagcgcggg atcatcggcg acgatcaaca gccggccgcc
    17641 ggctttgagt gtctggcgtt cgaggttcac atacgagtct tccacgcagg cccaaggctc
    17701 caccagcgcc acggcggccg cggccggttg gtcgctgacc gggatcagga atcgctcgcc
    17761 ggtggggtca ataattacgc gttcatccat cagcacgtat tcctggagcg cgccctcgaa
    17821 gttatagccg aaggcggagg cggagccggc ggtggggagg tctcgatagt cggtctgcgc
    17881 gatgcagcgt tccccgacag aatggtgctt gaccttggcg ccgacggcga cgatgcggag
    17941 ggtgacctcg tggcccagga cggtcggttt agagctgggc acatagctgg ggatttcgcg
    18001 cagcgcctcc gggctgattc ccttgacgac ggcgcctttg cgggggtgtt gatcaaattg
    18061 cttcaggagc ttgaggtcgg agaagcagag actcacgcat tcgatccggg cgagaacctg
    18121 gcaggggccg ggggggatga cgggcttggc gggattaagc gtgagttggc cggggccaac
    18181 cagttgaagc gcgtattgcg ttgtggggag tgcagtgttt ggcatgttgt attcctggca
    18241 gtgttacgag agcataacct ggccgcccgt gacaggcagc gcctggccag tctcgtattt
    18301 ttgttcgatg aggtagtaga tggcttttac gacgtccgcg gtggtgcagc cgcggttcat
    18361 cggaaccttg gcttcgtaga atcgcttcac atcggcaatt gtccgggccc ccggcacctt
    18421 gccgctgcgc aggtactggg cgaacaggcc gttctgggga tcggaccaga gggggccatc
    18481 gaagaaattg ccggggcaga tggcgttcac cttgatgcca tcctcaatca attccagcgc
    18541 gaatgactgc gtcaggccaa cgccgccaaa cttgctgccg gcgtaggcgc cgttgcgatt
    18601 cgagccttgc aaaccggatt tggagttaat ctggataatg tctgaagtgt attctttccg
    18661 tgccagatgc tgtatcgcca gaattggcgc ggctttctgc acgcaaagga agtagccctt
    18721 gtagtttacg gcagtgacaa agtcgaactc cttctccggc aatgtcttca cgctgcccgc
    18781 cttgagcacg ccggcgttgg aaataaatac gtccagcccg ccgaagacgc ggacgatctg
    18841 gtaaatggcc tcggccacgg aagcgccgct ggtgacattg attggcagcc ccagggcgcg
    18901 gggtttgccc aaccgggcat tgatttgcgc cgccgcggcc accgcgcccg cggcattaac
    18961 atccgtgagc accacggagg cgccctgggc ggccaggtcc tgcgaaattt ccagcccgaa
    19021 tccctgggcc gcgccggtga cgacgataac cttgccggcg gcgcgtccaa cggcctgtcc
    19081 cgcggcgctg acggtcttgc gatacgactc gacctcccag tcttcgatga acttgcgcaa
    19141 accgggattc atgtagcgga tgccacccag gcggcgggct ccggccatca ccttaatggc
    19201 gtcggtgtac acctgccgca cggtctcggc tgccgtgaga tcgtcgccgg cggcgaaaag
    19261 ccccaggccc ttgaccagaa caacgtgcgg cgctgaacgg gttttccgcg tatgctgctg
    19321 gattgcccgc cggagttgtt caactaatgc ggtggctggg gcgccggcgg caggctcaaa
    19381 ccacaacggg aaggacttgc aataaacgat ctgatccggg gtcagcgggc cggcggtggc
    19441 aacggccttg ccttgcgcgc tgtcgaccag gtccatcact tcgggcgcat cggaaaaagt
    19501 gaccaccttc aggttgtcgc ccgcagccag cagcccgcgc agggccggcc cgatggcttg
    19561 aatgagattg cgggcggcgg ccggttcgct cccggcaacc cggccaaacg gcggcgcctt
    19621 gccaaccgct gccaaccgct ggcgcagcgt ccgcaccagc cagtcggtat gccggcggat
    19681 ttgcgcgggg gtgtcgccgg agatggccag tccatgattc tgcatgagca cggcgcgtgg
    19741 acagcttcgg cccgtggctt tctggtagtc agtcagcgcc tgccaaaggg ttcgggaaag
    19801 aatataaccg gggtccacct cgggcaccca aaccacttcg gcggccaatt gccgggcgag
    19861 ttccgggcct tttacgcagc aggtcaccat attcaccagg gtgctgtgcg tatgcacaac
    19921 ataccgcccc ggcataagat gatgcagcag ggcttcgacg ctcgggcgct ggcgcttctc
    19981 cggatgaatg cgggcggcca gcgtcgcagc tttgaattcc gcctcgcgct ttagccggtt
    20041 gctggacagg gaactgtgca gcagactctc aagctgcagg cggtccatct caacaaaacc
    20101 ttcgggcgaa atggtggcca gggcatggcc gctgcctttg acgaatagcc gattgccaac
    20161 cttgacggag gtgttgccgc cgccggcaag gacaaactcc ggatcggccc cgtagaagcg
    20221 ggaaagcttc acgagtgcct ccaggggcgt gtcagatttg gtaaaaacgg gccactgtct
    20281 gcgggatgcc atagaggtat tattttgaat tcaggtaacg cccgataacc gtctcgccca
    20341 acgacttaaa cgccgagatg cgagccgcgg cattgggata attcggattg ggctggccct
    20401 gcgcgtccac caggccgcat tcgccgtgaa gcgtgagaaa ctgatgcgcg gccacgacgc
    20461 aggcgccctc ccaaaagagg tccttcagtt cttccggaac cgccaggcct ttcacgcagg
    20521 ttggctcgat gggaagcagg tccagggtgt tatgctcggt gccggcggtg accaccaggc
    20581 ccgccgcccg catcgcccgg gcatactcgg ttagcacttc cggggtgttg cggatcggaa
    20641 taaactcggc gcacgtgaag ccgcgagctt tgatattggc gatcagcttt ttcacgggct
    20701 gttcgaaagg gcagatgggg ttggtgccgt cggccagcgt agggtaggcg ggaatgccgc
    20761 ccaactccag aatcagccgc caggcctgtt caaagttgat aaaagtctcg ggcacatacg
    20821 cgggcttgcc cgcctttata agctgggtgc gaatttcgtt ttgaatcccg acggcatcct
    20881 cggggccgaa cttcggcgtc gtcgccaacc ccagaacttt cacaagctgc tcgatgcgtt
    20941 cgcccgcggg aacacgggca aagatcgctt cctgaaaagc ctggcagaca tgccgttcct
    21001 ggaggtagat gcgttggcgg gggcatccgt ggcgtcgggc caccatgtct atcacgtcgg
    21061 cctccgtcaa cggaataggc aggccgcgct gggccagcac ctcgcccagc ttgcggatga
    21121 cctcgcgcat ccgcgtgctg tcattgtggt ggatcaggct cagcaaccgg tttgcctccg
    21181 cgctgaacgg ttcaaggcgc gtaatgcctt tgccgcagat gtacatcttc ccaggattgc
    21241 cgggatcatt gatcttcacg ccctgctgca ccaattcgtc aatcaggcaa ataatctcca
    21301 gcccaaacag ggggaagatg ccggcctggc gggcgctttc ggcgaaatcg ccatacactt
    21361 cgtagtcgta gtagttgctg acgccaagaa cctggattcc ttgcgcaaca gaaagctcaa
    21421 tggcctgcgc cacagtctga aacgcggaga aattcggcgg caggtggata tggctgttga
    21481 ctttcagcct tttcaggcgt gggctgccgg acccggcttc ggccgccagg gccgtgggcg
    21541 ttcccaatcc tttaagcagg gcgagggctt ccgcggtgcg ttgcgtcata aaaatacttc
    21601 cagcaggcca atactactcc acaccggccg tgacctgcaa aaacttccga ggcggcaaat
    21661 gcagccccat tgccagcgcg tcttcggctg cttccttgat gccttcgctg atcgtcgggt
    21721 gcgcaaacac ggtttctgcg atatcctgca cgctgacttg ctgatgcaac agcgctccgg
    21781 cggcggcgat gcattcggtt gcattatgcc cgatcatatg cacccccaac aggcggccag
    21841 tttcacgatt gcgcaccacc tttacgaagc cctcgggatc attgaccgcc agggctttgc
    21901 ctagaaacga cagcgggaaa tttccgaccg ccaccgggaa gccctgggcc tgggcttctt
    21961 cggccgtcaa cccaaccgtt cccacttccg ggaaggtgta aacggcgttg ggaatcggag
    22021 ctttgaactc atgcccctga cccagcgcgt tctcaaccgc caccacgcca tgcgccgatg
    22081 ccgcatgggc cagcaggcac cgcccgttgg catcgccaat gcagtagtaa tcctttaaca
    22141 ccgtttccat gcgatcgttc acgcgaataa acccgcgttc ggtcgtcagt ccaatcgttt
    22201 cgagcccgat atcagcggtg tttggccgcc ggccagtggc tacgagcacc cggtcggtct
    22261 ccaggctttc gccgttgctc agcttcgcca ctgcgcccgc cccgctcaac gccaggcttt
    22321 cgatcttcac ccccgtaaat attcgaatgc cccgagccac gagtatcttc cgcagcgtct
    22381 gcgccacttg cccatccagg ttcggcagaa tctggggcag catttcgacc atcgtcactt
    22441 ccactcccaa cggctgcatc atgcaggcaa actcgcaccc gataacgccg ccccccacaa
    22501 tgagcagctt tcgaggcagg gtctgccaat gcagtgcttc gtcactcgtg caaacgacgc
    22561 gggtatctgt tggccatccc gggattctcg acggcaccga gcctacagcg agaatgattt
    22621 tatccgccgt gaactcctcg agcacgccag ccgccccgga gacgaccact cttcccgctc
    22681 cggccagggt ggcgcgcccc aacaggacgg ttgcctttcg cgcgccgagc aagcccttga
    22741 tgcctgcccg caatgtttgc aggattttat ccttgcgcgc ctgaatcttt ccccagttcg
    22801 cgaccgttcc ggcgggcact tcgagtccca tcgccgccgc atgctggatg cgatgcatta
    22861 actcggctga gcccagaatg gcctttgatg gaatgcaccc gttgttcagg caagtgccgc
    22921 ccaggtgatc cttctcgacc agcacgacgc gcgcgcccat ttgagcggct ttcaatgccg
    22981 caatataccc ccccgggccg gctcctaaaa tcgctatcgt gtaatgcttg ctcataatga
    23041 tccgtaatca gcctcagccc ggcttaggcg atcaactgga gcggattctc cagcaacgct
    23101 ttgagccgcg ccatgaactg cgctcccaag gtgccatcaa tcagccgatg atcggcgctt
    23161 aaagtcaggt gcaaggtttg cccggccttg agagccccgt ccttcacaat tacatcctca
    23221 cgcacggctc ccaccgccag gatcgccgcc tcgggcggat tgatgatcgc cacaaactca
    23281 tccaccccat acatgcccag gttggagatg gtgaagatgc cctgtcccat gttctcaatt
    23341 ctgccggcgc gggcctggtt ggccaggcgc tttgtttcca ctgctagctg cgccagcgtc
    23401 aactgctccg cgttcataac cactggcacc accaacccat cgtccatccc cacagcaatg
    23461 ccgatgtttg cccccgaaaa ctcgataacg ctctcgccat ctacacggct atgcatcgcc
    23521 gggaaatcca tgatcgcccg ggcgctggcc atgactatga tgtcgttcac gctgcatcca
    23581 accttggctt tctggctttt gtagaaggcc atcatcggcc cggcgtcaat acgcgcgcgg
    23641 agataccagt gcggaatggt ggtctttgat agcgtgagat tgcgggcgat ggctttgcgc
    23701 atgccgctca tacgcttgcg gatggccccg gccggcaatt ttccagcggc cacgggctcc
    23761 gccgctgcaa gcccaggttt gggcgtaccg gtggcgggaa tatccgtcga aagaatgcgt
    23821 ccgcccggtc cggacccccg tgccacggtt gccagattca ccccgcgttc ggcggcaagc
    23881 ttgcgagctg caggtgaagc ttttatccgc ccggtgccaa tggcggcagc gggaggcgcc
    23941 aattccgcgc tcggtttctc tgctgcttcc accggagccg cgggggctgc ggcaggtgat
    24001 gccgggctag tgctggaggc ggcaataaag gcgtcaacgt ccgcctcatt atccgccaga
    24061 taagccaccg gttgcagcac cgggctgctg gcgccttccc cggccacgat gcgcgctaac
    24121 cgcccggaat ccgttgcttc gacctcaatt gtcgctttgt ccgtctcaat ctcgaaaata
    24181 atttcgccct tcttaatcat cgccccgggc tggacgtgcc atttgacgat gacgccttct
    24241 tctacacttt gcccggcttt gggcatgagt atggggatta ctttgccggt ggaaatggtt
    24301 gtcttggtca cgggaataga tttttgcttg atgggttgag tggcagattg atttgccacg
    24361 ccttcaataa ccgccagagg tgccccgatg gaaatcgtgc ggcccgccgg agccagcacc
    24421 tccttaatta tgccatccat ggccgactcg attacagcca agccttcgtc cgtctcaact
    24481 tccagcagga tttctccctt gcgaacaact tgctctgcca gctttcgcca gcgaagaatc
    24541 gtcgctttag aagctgtttt gcctacgggt ggtaatgtga gcgtcggcat aaatcaacgg
    24601 gtctttgact gaacttaata cataactctt tcaatattca gctaaagaca gtcagcggtc
    24661 aatcagattt tgataaaaat atgttgcgtc cttgtcagaa tgaggttaaa ccatattcat
    24721 tgaatccgat aaggcggtcc cgcaacgctt ggatggccaa tctgggcggc gaggctgaaa
    24781 ggcagcagtt aaggttgcct ggctttggga taacttatga attcgtttcg tcagcggcgc
    24841 gagaaaatac tcgcggccat tttcgagaag cgacacgtct ctgctcgaga cctggcggca
    24901 agtttgcctg cgtccgaagc gacggtccgg cgcgatctca aggccttggc tgccgacggg
    24961 gagatcgaac tcgtttatgg cggggcgacg cttcggcgcc catccgatta ttcctttcgt
    25021 tccaaaggtg agcgtaacgt agaggctaag cggattatcg ggcggcttgc ggcagacctg
    25081 gtgcaagaaa acgagcagat ttttctggat tctggcacta catgcttcga aatgctgcct
    25141 tttttgaaga cccggcgcgg gctttcgatt atttgctctt ccgcccgact ggccctggaa
    25201 ctggacacgc cgggcgtctc ggtaatcctg ttgggagggc agtatcggcc ggaccgtatg
    25261 gatacggtcg gggtgctggc ctccgcgacg attgaccagt tgcgcggcta tctatgtttt
    25321 attggcgcgg acggcctgga ccgggagttt ggccccgcgg ccgctgattt ggaaagcgcg
    25381 gatctcaatc gccgcgccgt gcgcaatgcc cgccagaccg ttctgctcgt ggaccatacc
    25441 aaattcgccg ccgcttcgtt gtgcaaagtg gtggactgga aactggtttc ccgcattgtg
    25501 accgaccgcg ccccaagccc ggagtggatg gattttctga ccgcccaggg cattcaagtg
    25561 gattacccgg gggaataaag accccagact ttcaaccgcg ttgtttatga acatcaactt
    25621 accgcaaagg aactaacagc aattatgccc aagagcctat tcgtcaatcc cgtcgagaac
    25681 cgccgccccg gacagatcga attcaccccc attccgatca accagtaccg caaaacctgc
    25741 aaagaggaat tcgccgccaa gcgcttttcc aaggccgatt ttctgcgcat ccagcgcgat
    25801 atggagatta ttcgagcctt cgagaacatg ctcaacgaaa tcaagctgcg cggcagcttc
    25861 cacggcatcg aatacaatca ccgcggcccg gctcatcttt cgatcggcca ggaagccgcc
    25921 gccgtgggcc aggcttactt gctgggggtg gaggaccata tttacggctc ccatcgcagc
    25981 cacggagaaa tcctggccaa aggcctctcg gccatcgcca agctgtccga tgacgccctg
    26041 ctcaagatta tgcgcgatta cttcggcggc gacaccctgc gcgtcgtcga aaaggatgcc
    26101 accggctcca ccaaggacct ggccatagat taccttgtct atggcgcgct ggcggaaatc
    26161 ttcgccaaag actacggctt caaccgcggc ctgggcggct ccatgcatgc cttctttccc
    26221 cccttcggca ttatgccgaa taacgccatc gtgggcggct cgggtgatat ttcggtcggg
    26281 gcggcgctgt ataagcatat caatcgcaag cccggcatcg tgatctgcaa catcggcgat
    26341 gcctcggccg gctgcggccc ggtgtgggaa ggcatctgtt tcgcaaccat ggaccagttc
    26401 aaactgctct gggatgaagc ccatcgcggc gggctgcctt tgatcctcaa tttcgtcaac
    26461 aacttctacg gcatgggtgg ccagccggtc ggcgaaacca tggggttcaa agtcctggcc
    26521 cgcctcggcg ccggcgtcaa ccctgagcaa atgcatgctg agcgcgtgga tggctacaac
    26581 ccgctggcgg ttattgatgc catcgcccgc aagaaaaaaa tcatcgaaga caagaacggc
    26641 cccgtcctgc tcgacaccat cacctaccgt ttttccggcc attccccctc cgacgcctcc
    26701 agttatcgcg agaagtcaga ggtggacgcc tggcgccagc aggattccct cgccgcgttc
    26761 gcgcaggagc tcgtggacgc caaaatctgc acccgcgccg agattgacga gatacacgcc
    26821 caggccgagg cgatcattct caaggccttt aagaaagccg tggacatcaa gctcagcccg
    26881 cgcagcgatc tcaagctcaa ggattgcatc cttgaacgca cgatgttctc gaacttgaaa
    26941 atcgagtccc tggagccggg ccgcaaaccg gaagtgctcc tcaccaaaga ggaaaacccc
    27001 cgcgtcaaac agttggccca gcgcagccgt tcggccttcg acgacaaggg cgccaagctg
    27061 cccaagagca aatgcgtcgg tattcgggac gccatttttg aggccatcct cgaccgcttc
    27121 tatgtggacc ccacgctggt cgcctacggc gaggagaacc gcgattgggg cggcgcgttc
    27181 gccgtttacc gcggcctgac cgaggcgctg ccgtatcatc gcctctttaa ctcgccgatc
    27241 agcgaaggcg cgatcgtcgg ctccgccgtc ggctatgccc tggaaggcgg ccgcccgctg
    27301 gtcgagttga tgtattgcga ctttttgggc cgggccggcg atgaaatctt caaccaaatg
    27361 gcgaagtggc aatccatgtc cggcggcctc ctcaggatgc cggttgtcct ccgcgtcagc
    27421 gtcggctcga aatacggcgc ccagcactcg caggactgga ccagcatgtg cgcccacatc
    27481 cccggcctca aagtcgtgtt cccggccacc ccctatgacg ccaagggcct gatgaactcc
    27541 gccctcgtca gcaccgaccc ggtggtcttc ttcgagagcc agcgcatcta cgatgagccc
    27601 gaacatttcg tcaaaggcgg cgttcccacc ggctactacg aggtgccctt tggcgaaccc
    27661 gacatcaaga aagccggccg cgacctgaca atcctgacca ttggcgccac gctctaccgc
    27721 gccctggagg ccgccaaaca actggaggac aagtggggcc tgtcctgcga ggtcattgac
    27781 gcccgctcga tcgtcccgtt caactatgcc aaggtgatcg agtcggtaaa gaagacccgc
    27841 aaaatcctgc tggcatcgga cgcgtgtgag cggggcagcg ccctgcagac tatggccgcc
    27901 aagatcacgc agttcgcgtt cgacgaactg gacgcccccc ccgtcgtggc gggtgcccgc
    27961 aactggatca cgcccgccga cgaagtggag gacgccttct tcccgtttgc ggtggacatc
    28021 attgacagtg tccatgagca cttgctgccg ctcaagggct actcggtaac gcgggattgc
    28081 tccaacgcgg accaactgcg gcgttacgcc cagggcatct agaccaatcc agccggcgga
    28141 tgagtcaatt ccaggccatc atatttgaca tggatggtgt catcgtggac agcgagccag
    28201 tccacgagcg cgcctatctg gaggtcgtgc gcgaactcgg ctacggggat acccacggcc
    28261 tgcggtttgc ggattacgtg ggccgatccg atgaagtcct ttggcaggac ttcatcgccc
    28321 gccaccaccc gccgcagtcc tacgaggaac tgctggccct gaaaacccgg cgcgtggtcg
    28381 aaatcattcg ccgggaccag ccggtcttcg cgggcgtcgc cgaattaatc gaacagctcg
    28441 ccgcgcggta cgtgctggcg ctggcctcgg gctcggagcg ccagattgtg cgggaggtgc
    28501 ttggactcaa gggcctgggg cgcttctttt ccgccagcgt cgccggctcg gaagtccgcc
    28561 acggcaaacc ggcccccgac atcttcctcc gcgccgcgga aatgatccgg gtgccgccgc
    28621 gcgcatgctg cgtcatcgag gattccaagc ccggcatcgc ggcggggctc gccgccggca
    28681 tggaagtgat cgccatcacc aacacccacc ccgcggacga actgcgtgac gccacgcata
    28741 ttgtgcggac ttacagcgag attcagcacc tgctgctcgg gccgcaaagg cggtgcgcca
    28801 accgcgttcc ggatacgcgc acccacagcg ggtaacgggc cgggccgggc ggcggcgcgc
    28861 cgggcgcttc tggcagcaga gcggctgtcc ccaaaaaacc gaccgcagag agccaggcag
    28921 aaatggcccg actcgctcct ttcaactcgg ctgagggccc agaaggggcg gccctttacg
    28981 gcgttgcccg cgggcgggaa gcccccactt ggactggcaa cgggcaggcc aacatactcg
    29041 actaaaacgt tccacaagag taagcggata cggttatatg cgtagttcag gcagttgcgt
    29101 tcgtacacgc gctgctggag tgttggcagg gcgagcatga tctactgata ggaatttgtt
    29161 ttaggcgcgc ccgcacctgc tcagtgctgc gcgggtgagg gagaattggt ttttaagctc
    29221 accaggcatg tggcgagcct tgcgggagcc ggtggcggtg ccaatgcccg ttgttcgctt
    29281 tattcgcggt aattttcgcc cattcctggg tgtagtcgcc ggggtttggg ggcgcagcgt
    29341 atggcggggc ggagatcgcg acgggaacgc ccggcgcacg ggcgcgcccc gctccgagcc
    29401 gggtggatga tgcggcagtt tgagcggcgg cggcggggaa atgccggggc ggggcaacgc
    29461 cagttcgggc ggtcggccgc actggcgcac accgcgcatc ttgttcgagt accacccgta
    29521 gtagcgggtt atctgggcac ctttgccggg aacgtgccag gtggcgcggc gcaacccagc
    29581 atgaatcttc agccccaggc tcaacgtggc gcaaccggtt gagaaccatc tggcgggagc
    29641 cctggcccct tggaaatagg gcctgaccca tgcctttatg gaggggctca ataatgtgtt
    29701 cagtgccgtc aaaagtaagg cctacggcta ccgccccacc gtctgactcc tcttcaagct
    29761 ccacctgttg gatggcaaac tctgtatccc ccacacttga ttccactcaa aacgcagaga
    29821 aatcgtccga aaaccaacaa gtatctgaaa agaagcaagt tataaatgtg acgacttcgg
    29881 caatcccgaa gccaaccatt gccagcacct aagtctctcc tccgttattt caagccgtgt
    29941 acaaggggtg tacaacggat gctcaaggga agctcaagga aaaaaagcat gttcacccgg
    30001 tgagcatccc ttgtacaccc gttgtacacg gcctattggc agcactgaag tggctgggaa
    30061 tgctggagat gacgcaaagg cgggggtaat ggcgttgcgg gggagagtag ctgccaaaag
    30121 ggaaggcccg gaatgtgtcc aataattgga cagttgggac agttgggaaa gctgttatca
    30181 ggtatcgaag gcggagaaga ggtggcggag ttcttcctcg attttctccc gggaggggac
    30241 ggtgcgggcg gtttcggcgt gcgggattcc gcggcaacgg gtgcgcaggc tggggacggc
    30301 cattttcacc acgggcgcgg tgaggttcag gcgggcggcg atcccggcgt aaggccgggc
    30361 tgactctccc cgggcccaac agctttgtgg agactcgaac cgcctggccc ggccagaggc
    30421 cgcccaccct tcctgcaact gcgccatagc gtcctggagc agagtcatgg cccattgccg
    30481 gtcaaagagc aggccgggct ggatgttggg cgaggggtca gcggcaaagc gggattcggc
    30541 gaactccagg tcaatggcgg ttatccgcgc aaagccgccg cgcttttgtg cgcgttggcg
    30601 gtctcattca ttagccagga gccgtttcca gaccagcagg aggaaggtgc gaagcttgcc
    30661 cttcgctcgc gccgctacct gcaggcagtc tttctgcaac cggtggacga aggagcctgg
    30721 gcaaggtccc gcgcgccccg gggcgcatgg ccgtgccgcc gccggaaggc gcagaggggg
    30781 tgtccgcaag cgcggcaaag cgtctcgagc gcagtggccg acctgggcat gtctttttcg
    30841 cgcgccgggg gcacgaccgt ccagtggatg ggggtgaacc aggcgtctga agcgggcagc
    30901 ggtgcggggg cgacggaact tccgcccgcg gcggagggac ttgcgcggct cagtctgatt
    30961 caggaggccg ggctggcggc gggttcgtgt tgttggcgtt ggagttctcc gggaaaagcc
    31021 ctcgcaaggt gcgcaagggc cggaacggga acaggaaggg cagcgtgacc actttcggaa
    31081 ccaggtaaac cggatcgccc tttggagccc cgagcgtgcc ggtgatttta tattcaaaca
    31141 gcttcgtcac cggccacaag acggtgctga cgagcgggcc gaccagccag gcgtcgcgca
    31201 ggagctcggc ttccgcgcgg gcgttgacct ggccttgaaa gtccagggtg ccgcgatact
    31261 ggaggcgcat cccggtcgag cggatttcca ggtcttccga cgagattacg ccgttggtaa
    31321 tgccgaatgt gcaacggccg gcgctggccc ggctgttgcc cagccccggc accacgctgt
    31381 tgagcgcctg ggaaaagagg ccaaaaatgg gaacgtccca aatcaagccg ttccgcaaag
    31441 tcaggtcgcc ggagccggtc caggtgttta tatccgcggt gttggcgcgg gtgatggcca
    31501 gggtgccgtt aagccggccg tcgagatgat tggtcgcgag gaataaatcc gtcacgagcg
    31561 gcggcaggag cgcgttcgtg gccgtcacgg taaactggta gtcggcgtcg tgtccgggat
    31621 gaaagtcgaa ttgcgcggag ccgtgaacct ggccgccata gcagcccgcc cagatattgc
    31681 tcagggataa atgctgtccc agccagtgca cgtggccgga gatctccgac gcccggagcc
    31741 gccaccacgt gaaggcaccg ccgcgcaagt caaagtgcaa atcggcatca tcctccccgt
    31801 gcatcgggat gatgccccaa acccgggcga ccggcggctg ggcgaattgg tagggatgaa
    31861 tcacccgggc gacgtgaggg ccgatggcgc gcgcgatggc cagggggtcc ccggtgctga
    31921 aaccattgga gaggtagatt ttctgcgccg caaagtcggc tgtgacggca tcggcgctga
    31981 gccgttcggc gccgcgcccg aggcggggat ccgtgaagtg cagcaggtga ttggtgtatt
    32041 ggagggccgt ctgcaaatag ctggcggact cgccccggaa ggtgaaattg gtcagtgcca
    32101 cccggccctg gaggcccgtc cgccgcggtt cgtcccagcg cccccaaacc cgggcgtcaa
    32161 tgacaggcgg ctcggcaaag gtaaagaagc ggagcgcgcg ctgcagatcg ggggccagca
    32221 gcgggcgcaa atccagcacg ttcaccgagc tatgcaggcg gccgtgatac tcccgggtcc
    32281 gaagatcggt ttccacggcg gcctccaact gtccttcggg ccggatgatt cgcaagtccg
    32341 gcaggcgcca gacctggttc gagcaggaaa tatgggattg gacggcggaa accgccacgc
    32401 cctggcaggc gaggccccgg gcaaagttga gatcagcctg caagcggagc gtcggcagca
    32461 cttccgcccg ccagtcgggc tgacgattcg tccaggcggg ccagaccagc gaggcttcgc
    32521 cggccacggt cggcggcgtt tcccaggcaa gccgcgccag ccagtcgccg gccgctccgg
    32581 tcaggagcgg cgaaagctcg tggggatcaa tgctcgaagt aaagcggacg cgcgcctggc
    32641 gggtggatac gtccacgtcg ccgcgcccgc cgagcggctg ctgcgcgagc gcggcctgaa
    32701 tccgggtgag cgccagcgtg ggcgcgcgcc attgcccggc gcaggcgact tcacgcaggc
    32761 gtagttgaga cgattgcaac gtgtccgcgc ggccctccca gtccagcgca taaggttcga
    32821 gcgccgccca ttcgccccag gattcatcgg cgcggagcgg ccgatcgtgg ccgggcatcg
    32881 cgaggcgggc gttgatttgc agttcccgcg cagtggccca tgagttggac gcctgggcgc
    32941 aatgcaggct ggccgtgccg gacaggggct ggaaatcggc ccgcgagtgc atccattggg
    33001 cgcggaggtg cgcgttggtg gcgccgcccc agcgggacgc ggcgcgcgcc acggtgagga
    33061 ccgcatcggt ggtggccagg tttgtctggc ccgcgatcgc gtccgcctga agggcgagcg
    33121 tcaggtgcgc ggcgctcgcc caggaggaat ctgcgcgctc ggcacacaag gtcagttcga
    33181 ggtttcccca gttggttagc gacgcgaaag cggccagccg ggcattcagg ctcaaattcg
    33241 tcgccgtcgc ccagcgcgtt tgcgccccgc cggcttcgag gttcagctcg gcgtgggaga
    33301 ggtcattggt tgccgccggg aaaagccggg cattcacccg gccccggacc aggcggcccc
    33361 agggcgtgtg ggcctccggg gtgttcagca gcgcatggat ggtgaaactg tggggaacgc
    33421 gcgcgtcgcc ccggacatcg agtttgaact ccggcggggc ggagaaatgg atgcgttcca
    33481 acacctccgc gatttcgccg ggccagttgc gccgcggggc gccggccggg gccggttccg
    33541 cctgaaacgc cgtccaatgg cgcacggcgg aggcattggt gaccgtgccg gacaaccgga
    33601 ttttagcgcc ggcgaaaacc gccgtgaagt gatccagcgc ccattcatcg ccgggcagga
    33661 accggagttc ggactggatc tggtcaagaa ccaactgccg cggggcccgg tgggtttccg
    33721 cgacgggcca gaccaggcgg cctttgcgca gccggagcgc gtccgtctgg atttggagcc
    33781 gggcgagcgc ccgcagattg aggcgcacct ggacttgcgc gagcgtcagt tgcgggctga
    33841 ggggggcgtc ggggcgcacg aagcggacgt tctccgcgac gataccccga taccagctca
    33901 ggcgcaggcg ggagaattgc aaatccaggc cgcgagcgcg cagattgttc agcagcgggg
    33961 tcttggcaaa gccgggcagc ccggcctggt tgagataaat cagcgccccc agcacggcga
    34021 gaatcaggag ccaaaccgcc atgcggaacc ggcggaagta aacccagccg gcccgcccga
    34081 aactgccctt gcggcggttc gccatactca gcgggagggg gcgaacaggt agcgctgcac
    34141 gtaatcgtac agccagatcg ggaactcgaa aaaccagggc tccccattca gcgtgacggc
    34201 ggcggcgggc ggttggccgg gcgtttcccg ggcgaaatcg agcgcggcct tctcgccatt
    34261 ccggagctcg agcacaagct ggcggtcgtt gtccgcaaag cccaggcggg cgcggttggt
    34321 ctcgccccgg cccagccagg ccgccgcggt caactggcac agcccgctca cggtttcctc
    34381 gacggccagg tcgttcatcg cgcccgggga accgggggcc agcgaccagc tatagggtcc
    34441 gttgcggatc atctggcgga gctgccccgg ctgccggagg gtgacgcgcg ccacctcgtt
    34501 ggtggagagg ctccaaatcc ggcgttcgcg catctgccac cccgccgttg gcaggcgcag
    34561 gaactccgcg cgcggaacgg cataaacaaa gctctcgtcc gcgcgctgcg cgaagacctt
    34621 gtcggcctgg ttggttccga aacgcagttc ggcaatgacc gggttggtca gacctgccgg
    34681 gccattggtc gcggcggccc gcagtgtgta ttgccgcagc ggcgaatcca ggccgtaggc
    34741 gggcaaatcc gccgccacca ccacgtcctg ggtgaactcg acgattggca gttcgttcag
    34801 ggcggtgagc agctcgttga ccaggtcggc atcagcggga aaatcctccg gccaaacgcg
    34861 ccaggcgcca ttggtctgcc gctgcaaaga gaagctctcc ggcgcgcgca cctccacccc
    34921 cgcgacggga acggccgagt gcagcaggta gggctcgcgg aaatcattca ccgcggcata
    34981 ccagggcgcc agcagatctt tggacaccgt cacgatggcc tcaagcccca cccggcgggc
    35041 ataaaccagt ttggcgtcgt tggtggggct tttcccaaac tgcagccatg caatggtgtt
    35101 ggtgccctgg ccgatgacca cttcgagctc cggcgggtgc aggccaaacg tctccaggtc
    35161 cgccctgggg tcgtgggaga taaactgccg cgcgcgcaca ctctgcagca tttgcagcga
    35221 ttcctcgatc cgggcgttgt tggcgcgggc cgataacgga tagtgcatgc gccacagccg
    35281 gtcggtggcg gaacgccgca gctcgaaaat cttcgaccca ttggtgactg ccagccgttc
    35341 gaaggtcatg ccggacaaat ccagcagcgc cgtgctgcgc cagtcatcgg gagtgcgggg
    35401 gaggaactgc agaaatccgg cgtccacgac atagacccct tcgacgccga ccacctgcag
    35461 gaacacctgg tcgcccggcg ccgttcgcgc gccgactcgc aaccgcgcgg tatagccggg
    35521 ttgctcgata atgatggagg cctggggcgt ggtgaaccca aactcttcgt ccgcccgggg
    35581 ctgctcgcgc aaggcgcggg ccgtgaggta gggcgccggc accaactgct ccaactccgc
    35641 gagaaacttc tcgatgctca cggcctgggc ggggtaaacc agcggttcga caagctgcca
    35701 ggaaccgttg gtgcgcgccg cgcagatttc caggcgcgcg gcggggcgca cctgcacacg
    35761 cgtgaccgcg gcgggccgca gcgcggggag gaccgggccg gggccggaac tggtctcccg
    35821 caggtatttg tggtggaaga aaataaaggc catcagcgcc gcggccacga ttgcccatcg
    35881 ccagatgctg cggggattca tgcgcgcgcc tagttccgcc gccgcaacca gaccagcccg
    35941 cccagcagga gagcggagcc cggcagcacg cccaggagca accattgcgc ctggcgcatc
    36001 tgcgcctggg tcatgacgat gctgtactgc gtgatgggcc gcggccccaa acccgccagc
    36061 aggtgcgggc gctccagcag ccagttaatg gcgttgcccg caaaatcacg gttggcagcc
    36121 gactcgatct ggagattgcc caaaaacagg gagtcgccga cgacgatcat acgggtcgcg
    36181 ccccgctcgg cgatcagatc cttgatcgcg cctttctcga cggccaccaa aagcgggaag
    36241 ggcgcgtggt gggtggaatc gccggccccc cgggcccggg gaccggaaaa cgccagctca
    36301 tccacccgcg gcgcgtcggc ggcctcggcg cgcgccgtca accgccccac cgaccgcggc
    36361 aggctgacat gcagccgcga tccccgcaaa gaatccacta ccgcatgctg gccaaagcgg
    36421 ctgataatga tatccgtccc cttggcggta ttgtccgggt cggtgacgat attgcgcccc
    36481 acatccaccc cccattgggc cagcagggtt tccaaccccg tgttcttgcg ggccgcgcta
    36541 aagttaaaca aggccagcaa gcgcccgccc tggttgagat actgctcgat cttgaccagc
    36601 tcggcgtccg ggatggcgtc gcgcggcccg gcgatgatca gcaggttgca gtccggcggc
    36661 accgtattgg tgcccaggag cgacagcggc tcggcccgca cgcggttctg ctccagcagc
    36721 gacgcaaact tgaggtagcc ggtcacttcg tcgccgctat ccatttgatg ctcgccatgc
    36781 ccctggagaa aataggccgt caacggcctg gggctggtca cgtccagcaa ggcggaggta
    36841 aaggccatct ctcccagaaa cgccgttggc cggcgccgga acgaccagcc attggcgccg
    36901 accaccgcgt tggtcacctg ctccaccaca tacgacgcca gcgcgttgcc gtccaccggc
    36961 ttcaccttgc cctcgcagtc gaagatgacc aggtttttat cggtctgggc ggaaaggttg
    37021 tatttggctt tggttttttg cgccagcgcg ggattgcgga tgtagtccac ggtttccagc
    37081 cgaatcttcg ggttggccag ccggtattcg ttcagcagat cccacaccgg gctgtagagc
    37141 gcctcggcat cgtcggtatc atagtagagg atcacccgca cctggttggt caccgatttg
    37201 agcaggccca gggtgcgcgc ggaaagttcg atgcggctgc gcgcgctcag gtggaagcgg
    37261 agaaagtagt tcgcgctgag gtagttcacc atcaccacga ccgccaggac caataccccc
    37321 gccagcagca cttgcaggcc cgagccccat ttgcggccgg gcgtgaagct gggaggggag
    37381 gaggaatccg tcgccatggc tatttccagc ggcggctttc caccacccgc aaagtcagaa
    37441 agaggaaaag aaaggtcgcg ctggcgagca gcaccacggc ccgcgtatcc acaatgccgc
    37501 gcgcgaaatc gtgcatctgc tcgaacaggg ccaggctgcc cagcacctgc gtctgccaat
    37561 cctcctgcgc cggaagctgg ctggccaggt atcccagcag gaaaacgctg gcgccgaagc
    37621 ccaggctgat catggccgcg accgcctggc tgcgcgtgag cgccgaggcg cagcagcccc
    37681 aggaaaggaa catgccgcca tacaacagga tgcccaggaa cgtgctggcc gccacgccgg
    37741 catccagcgc gctgggatcg ttggtttggt ggcgcaccac cagcaggcag cccaggagcg
    37801 gcagccacat caccaaatag aacagcaggg ccgccgtgaa cttggccgcc accacctgcc
    37861 catcgctgac cggcgtggtc atgagcgttt caaaggtgcc ggtgaacttt tcctgcgcga
    37921 acagccgcat ggtgatcacc ggcgtggtca gcagcaggat cagccagaag aacggggtga
    37981 tataaaacag ctcggtgatg ggcatcggcg tcggcttctg cagtttggcg agaatgacga
    38041 tgaagctgta gcccatcagg aacagcgccg ccgcgatgat gatgtagccg cccagggaca
    38101 ggaaaaaccc cgccagctcc cgccgcacca gcgtccggaa aacgagcatc aaccctcctc
    38161 ctcctccggc cgggtgaccc ggacgtagat gtcttcgagc gaatggcggc tgcgggtcag
    38221 ctcgcgcagc ggccagcccc gctcccgcgc cagcgcaaag atttgcggcc gcaaatccac
    38281 gccgtcgcgc ggcgtcagcg cgcagcggaa atactcgccc tcggccgggg acacgtcgaa
    38341 ctgctccacc tccgccatct gcgcccagca ttcccgcaac tcctcgggcg ccgcggcgat
    38401 ctccgcgatc acctggctgc ggctgctcat aagcttttgc aggttccccg gcgtatcggc
    38461 caccaaaatg cgcccgtcat acatgatgac gacccggtgg caggtcattt ccgcctcggg
    38521 caggatatgg gtggaaatca gcaccgtatg ggcgccgccc agccccttga tgagctggcg
    38581 gaccacgcga atctggttcg ggtccagccc gatggtcggc tcgtccagga tgatcaactc
    38641 cggctcatgg accagggcat ccgccagccc cacccgctgc cggaacccct tggagagctg
    38701 cccgatcatc cgccggctca cctcggtcag cccgcattgt tccatgacca catccacgcg
    38761 cgcgcgcgaa tgtttgcggc tcaaaccttt cagccgggcc cggaacttca ggtattcccg
    38821 cacccgcatg tcatagtgca gcgggttgtt ctccggcata tacccgatgc ggcggcgcac
    38881 ttcgtcggat tgcgcaaaaa catccagccc ggccacccgc acggtgccgg aagtcgccgc
    38941 caggtagcag gccaggatgc gcatcgtggt gctcttgccc gccccgttgg ggcccagcaa
    39001 acccacgatt tcgccccgcc tgaccgagaa ggagatgtcc gaaacagccg gattgcccgc
    39061 gtagtgcttg gtcaggttgg cgacttcgat catcaagtca tcgctcattg agtccagctc
    39121 aggagaataa tctagttcag acccgcgggg ctaagccaac aaaatcggcg gcgtccgccg
    39181 cgctagcgcg ccaccagctc caccgtggcc tgccgcagca cattgaagtt ccccatccgc
    39241 tgcggcacgg cgatttccat ctgcacccgg tcgcctttct tccgggcgta aagccccttg
    39301 gccaccgcgc aaacatccat cggcagttgc ccgtccaagg cggtgatcag gatgccgcgc
    39361 tgcaaccccg ccgccgccgc cgggctgtcc tcctgcaccc cggcgatgta aaacgcatcc
    39421 gcaaccccca ccccatagcg ggcggccaat tgcggcgtca gctcctccag gtccagcccg
    39481 agcttgtcgc ggatcatccc ggcgttgaaa acggtcttct ccggcaccag ccgcacggtg
    39541 acctccttgc gctccgtccc ccgctgaatg gccagcgtca cgcccgaacc cggccgggcc
    39601 gccagcagat cggcaaaatc aatgaaactc cggggcgccc gcccattgac ctggcggatc
    39661 gcgtcgcccg cccgcaaacc cgcccgcccg gcggggcttt gcggctgcac actggtgata
    39721 accagcggcg tcgtgcccac cttgacccgc gcgccgaacc aataggtttt gacaaactcc
    39781 gtcgggaaaa tgtccgccag ggactccagc acgcgccgga tggggatggc aaacccgatc
    39841 ccctgcgcgc gctgccccat gacctcgttc aggacggcga cattgatgcc gatcagctcg
    39901 ccccgcaggt tgaccagcgg gccgccgcta ttgcccacgt tgatgggcgc gtcggtttgc
    39961 agccaattcg ggatgtccag cggcacgcct tccttgggca gcacccggct cttggagctc
    40021 agaatgcccc ggctgaccga gccgcccagg ccaaacgggt tgcccagcgc caccacggtt
    40081 tcccccagca gcaaatcatc ctcgcgggca aactggatgg ccgcaaactt ttcccccggc
    40141 cggctgttga gcttgagcag ggccacgtcg ctcttcgggt cgctggcgac gacgctggcc
    40201 gtgtaaacat tggttcccgt gcagaacttg atggtgatct tatcggcccg ccgcacgaca
    40261 tggtcattgg tgagcaagta gccctcctcg tcaataacca cccccgaacc gaggctgtac
    40321 tgcgagttgg gcggctgtcg gcggtgatac ggatcccaga attgccgaaa gaactcctcg
    40381 aacggatcgc gcacgcggat gagagtctcg gtggcgatgt tcaccacgca gggaatggcc
    40441 ttctcgacag cctgcacggt cgcgtcccgg cggacatcat ccggcgcggt ttgcccgatg
    40501 acgccgcaac tgccggcgca caacaccccc gcccaccacc aagctcccag ccgccgccgg
    40561 ctcttacttg tttgctgatt catatctata ataaccacca acccccgcgc gggttgcgcc
    40621 tcgcctcgcc gctcggctcc agccggcccc cgccgccgcg ccgcccggcg cccgggagcc
    40681 gccgcgccgc tcaaagcttg actttgccgt cccacccgcg caaaagcaag ggcaaggtgc
    40741 tttcggcgca agccagcttt tatgcccggt ggcgcgtgcg gtgcggagtc gtctccgacc
    40801 tgcgcgagga acgggactcg ccgcccgaac agctcctgca atccatctgg cagcaccagc
    40861 ggttgctccg cgagcagctc aaaaccttgg acggccgccc ggtccggatt cttcatcccg
    40921 gctttcgcag cgtcgagggc gggccggatt tccgcggcgc ggtcgtgcaa ttcggcgatc
    40981 gccccccgca aagcggcgat gtggaggtgg acctgcgccc ccgcggctgg cgcgctcacg
    41041 gccacgaccg caaccccgcc tttcagcgcg tcctgctcca cgtggtctgg gaagacggcc
    41101 cggccgcgcc cggcgcgccg ccgaccctgg ggctgcgccg ggcgctggat gccccgctgg
    41161 gcgagctgag cctgtggctg ggcggcgagg ccgcgccggg actgccggaa acgctgcgcg
    41221 gccaatgctg cgccccgctg cgccagcttt ccccggcgcg gctgacgcgc ctgctgcacc
    41281 aggcggcgcg ggtgcgcctg caaagcaagg caacccaatt tcaagcgcgc gcccggagcg
    41341 ccggctggga acaggcgttg tgggaggggc tcttccgcgc tttgggctac aaacacaact
    41401 cctggcccat gctgcgcctg gccgaactgc ggccccgctg gcagacgccc ggcgcgccgc
    41461 cgttcgcctt gcaggcgcgc ctgttcggca tcagcggcct gctgccggcc gagctgcccc
    41521 gcgcccccgc gggcggcagc cgctacgtgc gccaaatctg ggacttctgg tggcgcgaac
    41581 gcgacgcgtt ctccgactgc ctcctgccgc acaccctgtg gcgcctgcac ggctcgcgcc
    41641 ccgccaacca cccgctgcgc cgcctcgccc tggcctccca atggctgcgg gatgaccgcc
    41701 tcgcgccgaa actggaagca tggtgccgcc gcgaagtgcc ggcggcggct ctgcccgatt
    41761 cactgctgga gacgctgccc atcgaaccgg atgagttctg gtcctggcat tgcactttcc
    41821 gttcgccccg cctccgacgg gcgcagccgc tcctgggcgc cgcgcgcatg acggatttgg
    41881 cggtcaatgt cctcctgccc tggctctgga tgcgggcggc cgagggcggc aatgcccggc
    41941 tgcagcacgc cctcgaacag cgctatttct cctggccgcc cgcccaggac aactcagccc
    42001 tgcgcctggc ccggcagcga ttgctgggcg gcgccacccc gcgcgccctg cccggggccg
    42061 ccgcgcaaca gggcctgttg caaatggtgc gggatttctg cgaccactcg aacgccatct
    42121 gcgaccactg ccaactgccg aaactcgtgc aggactggat ggccgaggac ccataagccc
    42181 ggtgccggaa taaggacgtt gacctttcaa ccccttcgcg ccgggaaaac gaatacccca
    42241 gcccattccg acttctcggc aacctgaacc ccataacctc gggaatatac tccctcccga
    42301 accggacgcc caactgtgcc aatttggcac acctcagcct cgacagcagc gccccattga
    42361 ccctctcgtg cgagccactt tcgggtagct cctacaaacc attccatttc cgccagcatc
    42421 ttcattcctg ccaaaacccc tgcttttccc gtcccaatta gccatgtaca aggggtgtac
    42481 aagggatgct caccgggtga acacggaaaa atctgatgtg catccggtgg gcatcccttg
    42541 tacacccctt gtacacggct atggatcagc cagttgatag cgggaaatga tgggaagtgt
    42601 ccaataattg gacagtctgg gttgctgcgg cgaaaggagg gatgacgtgg ttgttggtgg
    42661 ccgattgcgt tgatagggtc gtcttggatt gctggctcgc ggcggaatta tccgtgggtg
    42721 attggtgtgc caatttggca cagttgatgg gcaatgcctg ggctggggag catccccggg
    42781 agactctttc ggcgggttcc cgccggactc aaggcagggc tttaacggct tcggttgcca
    42841 ggttcaggat tccgccgggg aacaggccca ggtagagcat tcccgcgatg caggcgtaga
    42901 tggccaggcg gatggggccg gaaaccggaa tgggggacag gtcggttgcg ttggcggacc
    42961 aatagatgac ccgcacgacg ttgaaatagt agtagagcga aatcacgatg ccggccaggg
    43021 cgaggaaggt caggtaataa taggcggcac aggcgggggc ttgttcgagg acggctttga
    43081 ggagcaggaa cttgccgaag aagccggcca gcgggggaat cccggcgaac gaggccatgg
    43141 ccagcgtcat cgtggcggcc agcaggggcg agcgccgatg caaccccgcc agggcgccga
    43201 ggtcttcccc gtccacctgg cgcagcgcga cacagatcac ggtgaaagcc gccagcaccg
    43261 tgaacaggta gccgcttaaa taatagagga ccgcggcgcg cccggcggcg ctgagggcgg
    43321 ccacgcccaa aagcaggtag ccggcgtggg cgatgccgga atagcccagc agccgcttga
    43381 ggttgcgctg cgggatggcg cacaggttgc cataaagaat ggaggcgccg gaaaggagca
    43441 tgagcaggtg cggccagtgc gccgtgatgt ccggcacggc gaggaagagc acgcgcagca
    43501 gcaggacaaa gccggccgcc ttggagccga tggccagaaa cgcggtggtc gggaccggcg
    43561 cgccctggta aacatccgga acccagatct ggaacggcac caccgcaatc ttgaagccca
    43621 gccccgcgag gatcagcacc aggccaaaga ggaaaacccg gttgccgccc agggcgcccg
    43681 cggcccgggc cagcccgtcg aagctcaggg tatgcgacag gccatacacc agcgcgatgc
    43741 caaagaccat gaaggcggtc gagagggcgc cgatgaccag gtatttcacg ccggcttcga
    43801 gcgacgccac ccggtggcgc tggaagctgg tgaggatgta gaaggtgatg gtgatcagtt
    43861 caagcgcggc aaagagcagg gtgaagttgt tggccgaggc ggcaaacagc atgccggcga
    43921 gggcaaagag aaccagggcg taatactcgg aaaggcccga cgtgatgcgg tcggcgaact
    43981 cgaccgacag gagcaggacg aggagcgccg ccagcaggaa gaagcgcttg aagaagagcg
    44041 ccagtccatc cagcacatac atctggctga acgcatagcc ggccgcgccc tccgggggca
    44101 cccgcacgca gaccaggctg tagagcagga tcaggcccgt gcccagcgcg gcgacgtagc
    44161 cgagtttgcg tttggccggg agcggcagcc ataaatccac gagcagcagc cccagcccga
    44221 gggcgagcac cagcagttcg tgactgagca acagtgtgtt catccgcgag aattacgggg
    44281 gaaggccccg gcccggcgga gcgccgccgg ggaggcgtcg gggggttgag gcgcgtcccg
    44341 gtccggggcg gggcgcgcga ggatccccgg gcgggcgggg atttccggcg ccagggcggg
    44401 ggcgactttt tcgctgacgg ccggcttgat tttgtcggtg agcaggcgcg ggtagcatcc
    44461 gaagatcagc aggctggcca gcaggaggag gaagggggct ttgcgccaga ggtgcggggc
    44521 gtcggccact ccggcccagg cttccggcag ggggccgtgc aggaggccgc ggatcgcccg
    44581 cagcatataa accgccccga tgaccagggc gccccagcag gcgaggatcg tcaccagccg
    44641 gtagctttgc caggcgccga agaagaccgt gatctcgccg gcaaagttgg caaatcccgg
    44701 caggccgcac ccggcgaacg cggccatcat cagcgccgcg ccaatgaacg gcagccgccg
    44761 cagcaggccg ccgagccgcg cgatttccag cgtgccggtc tgctgataca ggtagccgct
    44821 caggccgaag gtcagggcgg cgaggaagcc gtgcgcgatc atcaccagca ccgcgcctgt
    44881 gattccgacg aggttcaggg cggcgatgcc caggaagacg aagcccatgt gggcgacgct
    44941 ggagtagccc accaggcggt tgaagtcctt ttggcgcatg gccacccagc cgccatagaa
    45001 gaggttgccc aggcacagcc aggcgaggat cggcatccag tcgcgcgccg catccggcag
    45061 cagcggcagc gcgatgcgga tcaggccgta gaggccgaac ttcttgagca cgccggcatg
    45121 gagcatggcg gtggcggagg gggcggcgct gtagcccggc ggcgcccagg tgtggaacgg
    45181 ccagagcgag accaggatgc cgaacccgaa cagcagcagc gggaagatga agccctgggc
    45241 tccgggcgac aggggatgag cgcggagatg cgcggccagt tgcgggatgt cgaacgtgcc
    45301 cgcgcccgcc tgcacgtaga gcgcgatcag ccccaccagg gcgaggagcg cgccgaggct
    45361 caggtagagc gtgatttgga aggtggcgta gtccttttgc tcgccgcggc cccagacgcc
    45421 gatcatgatg aacgtcggca ccagcgccag ctcatggaag aagtagaagc agaagagatc
    45481 gagcgcggca aaggcgccga gaatgccgcc ggtcatgacc agcagcagga ggtagaactc
    45541 tttttcgcgc tcccggattt cccaggagac gcaggccgcg gcgaaggcca cgagcgcccc
    45601 catcaggacc aggccgacgt tgatgccatc cacgccgacg tggtagctga ggcccaggct
    45661 gcgcacccac gccacctgct gctcaaactg gtagccggcg gcggcggcgt caaagcgaag
    45721 gaacattttc accgccagca ccgccgccat caacgtggcc aggatggcga tgccgcggat
    45781 caccggccgg aaggtgcgcg gcaccagcag cagcacgatg gcggcgccga gcggccagcc
    45841 caggatgtag gtcagcggcg acatttttag aacaaaacca ggtaaaggat cagccccacg
    45901 cccagcgcga acaggagggc gtaggtctgc aagttgcccg tttgcagcag gcgcaatgcg
    45961 cgcccggcca gttcggtggt gccatgcgtc ccgcgcaccg ccacgcccgc aaggatccag
    46021 cggtcgaacc cgtcggccac ccgggccagg aaatcgtgcc cgcggatgac ggtggcctgg
    46081 tagagctcgt caaagtagaa gcggttgcgc atggcccgcc ccagcgcccc cagcatcccg
    46141 ggcagcggat cttgcgccgc gcgggcgtaa agcccatagg ccgccgccaa cccgatgagc
    46201 acggccagca ggccgctgcc cgccgccacc ggcgcgtgga ggaacggggc cacgaggtcg
    46261 ccgaggaacg aggcgggcgc cgccgccggt gcggcgcaaa gctgccgcgc atacagccgc
    46321 tcccagccca ggagcccgcc ggccacgctc agcagggcca ggattcggag cggccaggcg
    46381 atgaccggcg cggattcgcg ggcgtgcccc gccgcctcgg atttggctga gcccaggaac
    46441 gccacgaaga ccagccggaa catgtagagc gtcgtgaggg cggccacccc gatccccagg
    46501 gcgaacagtc cggggttgtg gtgcgcggcc agcgccagga tgctgtcctt gctgtaaaag
    46561 ccgctgaacg gaggcacccc ggccagggcc agcgtgcccg ccataaaggt ccagaacgtg
    46621 acgggcatct tttggcgcag cccgcccatg cgccagatgt cctgctcgtg atggagggcg
    46681 aggatgaccg agccggcccc gaggaacagg agcgccttga agaaggcgtg ggtggtgagg
    46741 tgaaacatgg ccgcggcggg gccgcccagg ccgaccgcca tcaccatgta gcccaactgc
    46801 gagagggtgg aataggcaag gatgcgcttg atgtcgtttt gctggagggc gatgacggcg
    46861 gataagaggg cggtgaagcc gcccagccag gcgatgaggg tcagcgcgcc gggcgtgaag
    46921 atgaagaaca cccggcagag catgtatacg cccgcggcca ccatggtcgc ggcgtggatc
    46981 agcgcgctga ccggggtcgg gccttccatg gcgtcgggca gccagacgtg gagcgggaac
    47041 tgggcgctct tgccgatggc cccgcagaag atcagcagcc cggcgccggt caggcaccag
    47101 gtttgccccg cgcccgttgc cagcccgtcg ctgatttgcg cgaagttgag cgatccgaac
    47161 ccggcccaga ccgtcaggat tcccagcagg aagccgaaat ccccgaggcg gttggtgagg
    47221 aacgcctttt tgcccgcctc ggccgccgcc gggcgctcga accaaaagcc gatgaggaga
    47281 tagctggaga cgccgaccag ttcccagcag atgaagaggg ccaggaagtt gttggccagc
    47341 acgatgccga gcatcgagaa ggtgaacagg ctcaggcagg cgaagtagcg cgcgtagccg
    47401 cggtcgccgt ccatgtagcc ccaggaatag atatgaatcg cgctggccac gccggtgacg
    47461 acgagcagca tgggcaggct cagcgggtcg aaacgcaggc cgaattccac atgcaggttc
    47521 ccgatcgcca gccaggtgaa ggcggagtcg ggccgggaat cccagccggt cagggcgacg
    47581 tagaggaggc tcaggaggaa tccggcgctc acggccccga tggagagggc ggcgctcagc
    47641 ttgccatggc gcgacgtgaa cagcgtgatc cccgccgccg ccagcaacgg caggaacaga
    47701 atgatccagg ggatgaagtc caggtgcatc ggctagagtt tcagcagggt caaatcttcc
    47761 acgtgcgccg tctgccgctt ccggtaaagc gccacaatca gcgccagccc cacggccacc
    47821 tcggcggcgg cgaccgtgat gatgaaaaac acgaagacct ggccatcgag gttatgggtg
    47881 aagcgcgagc aggccaccag cgccaggttg gccgcgttga gcatcagctc cagcgacatg
    47941 taaatgatga gcaggttgcg ccgcgcgatc acccccagca gcccgaggca aaacagcagc
    48001 atgctgaccg ccaggtaatg ttccaggcca atgttcattt caggtccttc ttgctgagca
    48061 gcaccacgcc caccatcccg accaggagca gcacggacac cagttcgaag ggcagcaggt
    48121 attgcgtgaa caacagtttg gccagggcgc gggtgccgcc ctcgagcacc ggcgcatcgg
    48181 ccccgcccgc ggcggccggg tcccgggagg ccgcccacag gctggccagg aaaatcgcca
    48241 gcagggcgcc cgccgcgagg acgccgcctg ccaccccggc ggctttgagc tttcgctgtt
    48301 cctccgcttt tagatccagc agcatgatga cgaacaggaa cagcacgatc accgcgcccg
    48361 catagaccag cacctgcacc gcggccagga agaaggcgtg cagcagcacg aacagccccg
    48421 ccagggagat aatggccagg acgagaaaca tggcgctggt caccgggttg cggctgaacg
    48481 ggttggcgac caccagaaag ccaaagacca gcgtcaaaaa cgcgaagaga taaaagagcg
    48541 cgtcctgcat gggctagaga ggagtgttgc cggtcgtttt accgctgaat ctgttgcctt
    48601 ggttcatggg tgatccgcgc ctttccgctc agggtttctg cccctgcgcc cgggcttcct
    48661 gggctttctg ctgccatttc tttatcttgt cctggtgcac ccccccgagg gcgagcagtt
    48721 tctctttgtt atagatcatc tcctcccggc tggcgccgat gagcgaatag tctttcatca
    48781 ggaagatggc ttcctccggg cagacttcct ggcaatagcc gcaaaagatg cagcggagca
    48841 tgttgatttc gaactcgcgc ggcattttct ccgcattggg gcggtccgcc ggcggccccg
    48901 cggggccggg cggggtgatc ttgatggctt tgggcgggca aacaaactcg cagagctggc
    48961 aggagacgca cttggtgttg ccgtcctggt cccggacgag gtagggcgct ccgcgatagc
    49021 ccgggggcac cacccagcgc tgctcggggt attgcatggt cacctttttc cggaagaaat
    49081 ggcgcaggct gactttgaat ccgccgataa tggccggcag ataaagccgt tcccaccaac
    49141 tcattttctg gcgttcgatg atcatttaac ttcgtatcca gagcacggcg gccgtcacca
    49201 cgatgttggc cagcgccagc ggaataaacc ggcgccagcc caggtccatg agctggtcat
    49261 accggaaccg cggccacatc caacgcaccc agatcaccat gaaaatcagg aagcacatct
    49321 tggccaggaa gatgccgatg tgcagcagcc cggctccgat cgtcgccgcg ggctggttca
    49381 ggccggccac cggcaggctc cagccgccca ggaacagcgt cgtcatcagc gccgcggcgg
    49441 ccaccatgtt ggcatattcg cccatgaaga agagcgcgaa cttcatcgag ctgtattcga
    49501 cgttgtagcc gccggccagc tcctgctccg actcggggag gtcgaacggc attcggttgg
    49561 tctcggcaaa catggcggcc aggcaaatga caaacgacac aggggcatag aggatgaacc
    49621 agccgtgcag ggcctgccat gagaccagcc gccccaggtt caggctgctg gtgccggcca
    49681 gcagcttttg ccagcccgcg ctggccgccg gcgcttccag ccccccggcg atgaggaaca
    49741 gcgggatcac cgccatgccc agggcgatct cgtaggagat gagctgcgcg ctggagcgca
    49801 ggccgccaat gagcggatat ttcgaccgcg ccgcgtagcc ggcgaggaca atgccataga
    49861 cgcccagcga tacgatgccg aaggtgtaaa gaatgcccac gttcaagtcc gcgatgacca
    49921 tcttttggcc gccgagcagc gagccaaacg gaatgatggc cacggtcagc aggctgggaa
    49981 tcatgacgat cgccggcgcc agccagtaat agaccttgcg cacgtgggcc ggcgtgaagt
    50041 cctccttcag gaaagacttg atggcgtcgg ccacgggctg gagcagcccc aatggcccga
    50101 cgcggttggg gccgacgcgg tcctggatga acgccgcggt ccggcgctcc acccagacgg
    50161 cgtaggcgac aatgaacatg accaccgaga acacgcccag aatcttcagc gccccgaaca
    50221 acaccagttg tccggcctcg gtctccgccc agttcagaat cgccggaatc atggctcgct
    50281 ccggagggtg gcgccctggt cgcccaggcc cgcccaggtc agccccgcga aggccggcag
    50341 gtcccgggcc atctggttaa acagtccttc gaggctctgg cagccgtccg ggccggccac
    50401 ctggagcgcc aactcgtgca ggaactccca ttcggggcgg gcgtcgccgc ggggttcgac
    50461 cgccttcatg aacttttgca cccgcccgcg ggcgttggtg aaggtccccc gcttttcggc
    50521 atgggcgcag ccgggcaaca ggtaatgcgc ccgccgggtg gtttcattgg gcagaatatc
    50581 gctgacgatg agcgtttcca gccgggccag caggtccgcc cccaacccca gccgggtcac
    50641 ctcttcgccc accaccagca aggtcttgat ggttccggcg cggatgccct ccgcgatctt
    50701 cggcaggttc gcccccatcg gcgtggcggc gatgccggtg agccgggcgc ccgcgctatt
    50761 cgggttgcgg tcggcgttca gcaacagccg gtcgccctct cccatgcgcg gcaccgaatc
    50821 cgtcagcgcc ccgagcttct tcgccagctt cgccagcaag taaagttctt cgttggtctg
    50881 ccgggccgag gcgatgatcg ccaccgagcc cgccgccgcc tggcgcagct tcccggaaat
    50941 ctcccgcaag attggggtcc agccgggcgc gggagcctgg ccggcggcct gcgggccgcg
    51001 gatttccgtc aggcgatccg cccgcccgat ccacgcgtag ttcagccggc cgtaatcgca
    51061 catccagcag gcattgacgg cctcgttctc gcgcggttcg tagcggtaga tccggtcttc
    51121 gcgcgagccg atgacgatgt tgcagccggt ggcgcaactg gtgcaaaggc tcggggtttc
    51181 cttgaggaac cagacacgca tctggaagcg gaaatccttg gaggtgagcg cgcccacggg
    51241 gcaaatatcc accgtgttga gggtgtaatt gttgtcgaac ggccggccgg ggtaagccgt
    51301 gagcgtgctg tagctgccgc ggttgacgag gcccagcgcg tcgtcgccca caatgtcccg
    51361 cgtaaaccgg atgcagcgcg tgcatagaat gcagcgctcg tcgtccagca cgatgcgcgg
    51421 gcccaggtcc acgcgtttgg gcttatgcac cttgggttcg acgaagcggc tctgcgcttc
    51481 gccgtggtcc tccgagtatt cctgcagctt gcattcgccg gccttgtcgc agatcgggca
    51541 atcgagcgga tgattgataa gcaggaactc cagcacgctt tcgcgcattt gcttcacggc
    51601 gggcgtgtcg gtgtagattt ccatgcccgg ggaaaccggc gtggcgcagg cgatggcggg
    51661 gcgcggcgac ttggtgatgc gcggcgcgcc gtccgggttc aggatcggct tgcgatccgg
    51721 gcccagggcc ggcgtgccaa actcgaccag gcacatgcgg cagttgcccg ccaccggcag
    51781 cttcgggtgg tagcaataat gcgggaccat gacccccgcc gcaaagcagg cctggatcat
    51841 cgtggttggc accggcttgc cggaaaccgg gtccggcatc gtccggggca cttccaccgc
    51901 cttgccgttg acccgcacct ggagggtgtc cgcgggcggc ggcgttgacg gggatggcgt
    51961 ggggttggcg gtggacatgg tcattcggcc ggggtttggg ccagcgcggc cgcggtcgcg
    52021 gggtgcggcg gcccggaggg cgcagccgtt tcccgcccgc tggcggcccg gcgtttcgca
    52081 tccgccgcgc cgcgggcgat gaattcgtcc cggaacttgg cgacgaagct ctgcacgggc
    52141 caggcgcagg cttcgccaaa ggcgcaaatc gttcgcccgc cggggatgtt gtcggcgatg
    52201 tgggccaggt aatccgcgtc ctccgggcgg ccttccccgt gcgtgaggcg gcgcagggcc
    52261 ttggccatcc acagcgagcc ttcccggcag ggtgtgcact ggccgcagct ttcatgggca
    52321 tagaagtcgg cgatgttcgc cagcgccgcc acgatatccg tggagtcgtc cagcacaata
    52381 atcgcccccg agcccgacat gctgcccgcg gcaatcagcg aatcgaagtc gtagggcaaa
    52441 tccagcagcg ccacttcctg cacgctctcc ttgccgtcag ggccccggcg cttgagcttg
    52501 aacgtttccc ccgccttgaa gaccttggcc gacgacccgc ccgggatgat cgctttgagc
    52561 tgccggccct cgcgcaggcc gccgccaaat gccgggtcgt ggatgagctc gcccagcgtc
    52621 accttgccga cctcgatctc gtaatagccc ggccggcgga cgtggccgct gatgctgacg
    52681 atgcgggtgc cggtgttgtt gggggtgccc aggcgggcgt attccgcgcc gccccgcgcg
    52741 acaatatgct tcaccgcgca gagcgtctcg acgttgttga caatcgtcgg gcattggtaa
    52801 agccccagca cggcgg
//
LOCUS       DAOPQG010000007        50343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_881_length_50343_cov_14.227549, whole genome shotgun sequence.
ACCESSION   DAOPQG010000007 DAOPQG010000000
VERSION     DAOPQG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 50343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50343
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_881_length_50343_cov_14.227549"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..3088)
                     /gene="uvrA"
                     /locus_tag="PKI07_01420"
     CDS             complement(53..3088)
                     /gene="uvrA"
                     /locus_tag="PKI07_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOC49739.1"
                     /translation="MSKAFIRIGGAREHNLKNLTLSIPRDKLVVITGLSGSGKSSLAF
                     DTIYAEGQRKYVESLSAYARQFLDQMQKPDVDFIEGLSPAIAIEQRSSGSSPRSIIAT
                     TTEVYDYLRLLYAHVGQPYCPETGVPILRQTTSDIVDKILALPPRTRVMLLAPVVRDQ
                     KGEFRDVVARLAREGFVRARVDGQLVELGGGVRLKLDPKARHTIEAVVDRLVIDEKIR
                     VRLSDSVETALRWGEGTLLTLHQTPPEDRTARAESRPAPHGPRAASPAPPANSEWVET
                     LLSNRNLSPATGKSYEPLTPKHFSFNAPAGACPVCHGLGQKLVFDEGLVVPDPEKSLE
                     QGAIQPWRRGGKRMVLYYKSLLQGVAKHYQQSMDAPYQTLPEDFQRTLLWGSGETQIE
                     FLFRRMGATSKVTRPFEGVIPNLQRLYQESESEFTRNRLKGFMSPQFCDACQGQRLKP
                     EILAVTLGDAEASAKFKPAGRETGRESEAVPRPPSPRVSPPGLSIMDVCTLSVEQADE
                     FFATLKLSEFQEKIAQELIKEIRARLGFLRNVGLGYLTLDRESGTLSGGEAQRIRLAT
                     QIGAGLVGVLYILDEPSIGLHQRDNDRLLRTLAGLRDLGNSVLVVEHDADTIRHADYL
                     LDLGPGAGVRGGELVAAGTLAEVLAHPHSLTAKYLRGELSIPVPRRRVQPSAARGWLE
                     VLGARANNLKNLNARIPLGTLTCITGVSGSGKSTLVDDTLRRALGRKFFGSKERPGAH
                     RALRGFENLDKVIVIDQTPIGRTPRSNPATYTGMFNHIRHLFARLPDARIRGYGAGRF
                     SFNVKGGRCEKCQGDGLLKIEMHFLPPVYVTCEGCNGRRYNRETLEINYKGKNIADVL
                     DMTVDEAATFFRAVPQIYEPLLTLAEVGLGYIGLGQAATTLSGGEAQRVKLAAELSRK
                     ATGRTLYILDEPTTGLHFHDVAKLLEVLFKLRAAGNTLIVIEHNLDVIKTADWVLDLG
                     PEGGEAGGQIVAEGPPEVIAQCPASHTGQYLAAVLARPS"
     gene            complement(3303..3767)
                     /locus_tag="PKI07_01425"
     CDS             complement(3303..3767)
                     /locus_tag="PKI07_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOC49740.1"
                     /translation="MKIGVLSDTHNYLDPRIPRLFADVDHILHGGDIGLPIVLLELEQ
                     IAPVTAVGGNTDDPGLGYPETAIALLEGRKFLLQHIVKPQAPAEPLRARLARERPDVV
                     VFGHTHKPFCQRLGGTLFFNPGYAGKPRFGLERSAAILHCQPDEIRPEYLRL"
     gene            complement(3872..4489)
                     /locus_tag="PKI07_01430"
     CDS             complement(3872..4489)
                     /locus_tag="PKI07_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49741.1"
                     /translation="MPGEPNRKWHSWLPYLFLLIAALSRWPGLFPPNFSAFYGLAFCA
                     GAFFPRRVRWWLPLGTLVATDIALNFYYQTVSALQLVNYAAYALIILLGTRFNHRSRF
                     LGLLAGGLLGAILFYLLTNTASWLSNPEYPKDLAGWILALTKGTSGYPPTFEFFRNTL
                     MSGGLFTALFAGALKLTEAPEPASEKEPAPEEEPEGEEAPEEAPA"
     gene            complement(4529..4798)
                     /locus_tag="PKI07_01435"
     CDS             complement(4529..4798)
                     /locus_tag="PKI07_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49742.1"
                     /translation="MTKTAIFASLNARMKKKPSRQYRWQQKQIALNRCARCGAERTHY
                     AILCDACHIADRIRQQERSGSRPWQKGKRGRPPIVPLPEKKAALL"
     gene            complement(4855..5772)
                     /locus_tag="PKI07_01440"
     CDS             complement(4855..5772)
                     /locus_tag="PKI07_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC49743.1"
                     /translation="MFRNLSRRAALRRLTEGTAALAALTRLPSPGAAAEEPAPPALKR
                     RIRHSVSAWCYASLFRAGKDKPASMTFEDFCRACQRLGLESVELLNEDQWPAVKKAGL
                     TCAMCNGPDSIPYGWNRLEHHDDLLAKFEVAIPKVAQAGFANIITFSGNRKGMSDAEG
                     LANCAKGLKRLMPIAEKHRVTVCLELLNSKHSHKDYMADHTAWGVELCRQVGSERLKL
                     LYDIFHMQIMEGDIIHTIRESHPYIGHYHTGGVPGRNEIDNTQEIYYPAIMQAIVDTG
                     YKGFVGQEFVPRRPDPLASLKQAIQICDV"
     gene            complement(5836..7719)
                     /locus_tag="PKI07_01445"
     CDS             complement(5836..7719)
                     /locus_tag="PKI07_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOC49744.1"
                     /translation="MKLVSGVLPVSAHRLIVLGALLAATFAGLGDEPPSRQDILKKVT
                     PPPKHPRLFWTARDEAGVREKMNADPQLAAIGEAVRITAEAMLPSSPVVYRKTGRRLL
                     GRSQEALNRIMHLGFAFRLTGDQRYADRALAEMKAMADMPDWNPSHFLDTAEMTLALA
                     VGYDWLFDQLAPELRQASRTAIEQKGLAPYLESETRRGWEHGRNNWNQVCHAGMVAGA
                     LALLEDHPDQAAEVVHRALAGLPYALKAYVPDGIYPEGPGYWNYGTSFNVMLLSMLES
                     VFGTDFGLSRSPGFLQTGEFMLNIIGPTRRTFNFSDCGSGTGFSPAMMWFAARTERPE
                     LLWFENELLEQKLASIRSSHGRQSGGRYFPLVLLWAKAGMKPRQPAARNWLGQGQNPL
                     AVFRSSWTDRDAVFLAIKAGTPAASHAHLDIGSFVLDANGVRWSVDLGAQDYNTLEQR
                     GIRLWDFQPGSDRWTLFRYHNRGHSTLLIDDADQVVTNTAPITAFSADPENAYAVVDL
                     SAIYAGQVARASRRFTLQPDRRVVIEDQLTGGAQPVKVRWGMVTPAQLKPDGPSRAWL
                     EKDGKRLRIEIISPARATLESWPANPPPRDYDQPNPGISVVGFTVPLAAGETLSLKVR
                     LSP"
     gene            8248..8967
                     /locus_tag="PKI07_01450"
     CDS             8248..8967
                     /locus_tag="PKI07_01450"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC49745.1"
                     /translation="MESTVEQLPILHTLWAWFDANKKPVIVGTAIVGVAALIISFVVY
                     HRNATEVAASEALSKVPMEHMRATGGNTISADAYLKVAATYPKSAAAGRALLQAAGIL
                     FTEGKYPEAKAQFERFRREHAGSPFMGQALLGIASCLNAQGQSREAMTAYKELIDRRP
                     GDSTLAQARFALARLHEAQNEPDKARSLYEEVERTDAYGSLGDESRMRLEDLRRSHPN
                     LFAPAAAAGSNSAPFKVVAPQ"
     gene            8964..10253
                     /locus_tag="PKI07_01455"
     CDS             8964..10253
                     /locus_tag="PKI07_01455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOC49746.1"
                     /translation="MNLSIIGTGYVGLVTGACFAEVGHRVVCVDNDAAKVRTLQAGGI
                     PIYEPGLEELVARNRDAGRLTFTTHTAEGVEQSDVVFIAVPTPPLPDGSVDLSYLEKV
                     AREIAAAMTRYKIVVDKSTVPVKTGDKVAETIKRYGKAKVDFDVVSNPEFLREGFAVE
                     DLMRPDRIVIGVRSPRPVAALKQIYAPFDAPIIVTDINSAELIKHASNSFLALKISYV
                     NALSVVCEATGANIQEVANGMGMDARIGRRFLQASLGFGGSCFPKDLSAFIKIAEQVG
                     YDFRLLKEVQRINADQMERFVKKITDTLWVLKDKTIGVLGLAFKQNTDDIRMSPALEL
                     CHRLQKEGAALRVHDPKAMEKARAVLKDVTFVADMNAVAEGCDALVIATEWEEFKQLD
                     LERVRRSLTHPILFDGRNLFDLAEMERLGFIYKSIGR"
     gene            10302..10766
                     /gene="mutT"
                     /locus_tag="PKI07_01460"
     CDS             10302..10766
                     /gene="mutT"
                     /locus_tag="PKI07_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HOC49747.1"
                     /translation="MTPSAPAGGRSSPGVPASCAPAVEVAAALVFRDGRLLITQRPAA
                     AHQGGLWEFPGGKREAGESFEDCLARELREELGIEVAVAESVASLTHAYPDKTVHLRF
                     YRCRWLRHEPRPLGCAAFAWVTASELSRYAFPAADARLLAKLRDEAGLWRTP"
     gene            complement(10835..12007)
                     /locus_tag="PKI07_01465"
     CDS             complement(10835..12007)
                     /locus_tag="PKI07_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOC49748.1"
                     /translation="MPQQLAYVIITPYSLHKSRTGGILTRLITRTGCELVAARMFAPS
                     AELVKEYARETISADDPQDRNIQELIYDYILRNLAPDPKTGRRRRVMVLLFQGEDAVS
                     KTRAVVGNVCPDRRVGETIRDTYGDLITDEHDQLRYFEPAVLAAPTLAEAEKKLKLWA
                     HYADTDGGVLKDVIVYQPGEKPQQTLVLIKPENFRFATGRPGNMIDFFSRTGLYIVGV
                     KVHRMSVAQALQFYGPVRETLRTKLKDVVAAKAAAALEKEFGFAIAREQQEQLGALLG
                     PSFGDNQFDNIVRFMSGRSPAECPPGEEEKPGSEKCIALVYEGVGAVAKIRDVLGPTD
                     PSKAPPGSIRREFGQTIMVNAAHASDSEENARREMGIVNVAENNFRQVVEQFYGKT"
     gene            complement(12086..12226)
                     /locus_tag="PKI07_01470"
                     /pseudo
     CDS             complement(12086..12226)
                     /locus_tag="PKI07_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301710.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
     gene            complement(12350..13147)
                     /locus_tag="PKI07_01475"
     CDS             complement(12350..13147)
                     /locus_tag="PKI07_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC49749.1"
                     /translation="MFPLKDKWILITGASSGFGAAAGRAFAAHGARLLLGARRLERLQ
                     QVAAEARQAGAPEAHAHVLDVRQTASVEAFVNWARSRISPRQPADARLDVLINNAGGA
                     HGLDPVAQGKDEDWEAMLQINVLGILRMTRAALPLMERNPGSTIINVGSLAGHVAYEG
                     GAAYCAAKAGERQITRALRLELNGTGIRVCSVDPGMAETEFSLVRFKGDAARAQQVYA
                     GTQPLTAEDIAEILVWVASRPPHVNIDELLVKPTDQAAIHKVHRRPA"
     gene            complement(13243..13563)
                     /locus_tag="PKI07_01480"
     CDS             complement(13243..13563)
                     /locus_tag="PKI07_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49750.1"
                     /translation="MSKPPMEGYSLSAMIGAIVGSIGGLFAIGGVRAILAHNLTLVFR
                     FPILGFLCFLICGLAGWFLGGFLGPRCGQRFGSYQAELLGGAAGGLAPVLLIAIWAWY
                     TSPH"
     gene            13656..16037
                     /locus_tag="PKI07_01485"
     CDS             13656..16037
                     /locus_tag="PKI07_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOC49751.1"
                     /translation="MTKPSRSMNLLLRCFAFSLLLSLSAGQGAPPPAGVAPSFDPGKL
                     AEMDAAIEQAIADKKCPGGVLWLEHRGASYHKAYGRRALVPAPEPMSEDTIFDAASLT
                     KVVACTPAVMLLVERGQVNLDATVQTYLPEFKGQGKEAITVRQLMTHTSGLRGDIETK
                     TDWHGQQTAIQKACAEKLLSTPGTAFCYSDINFFLLGEIVQRVSRQLLEKFVAQEIYR
                     RLKMADTGFLPPAASLGRVAPTEVVAGRPYRGVVHDPTARKMGGVAGHAGLFTTAADL
                     ARYARMLLNRGSLGGARLFKPETVDLMTRVQTPASVPARRGLGWDIDSGFSGPRGKLF
                     PLGSYGHTGWTGTSLWIDPFSQTFLIFLSNRNHPSESGNVGALRAKLGTLAAEAIRDF
                     NFAYVPGALAAASPPEAPAARREARPAQLKTLNGIDVLVRQKFAPLKGLRLGLITNHT
                     GEDRDRNPTIDLLKNAEGVELKALFCPEHGIRGTADEKLGDDVDAKTGLPIYSLYGQR
                     SKPTPEQLRDLDALVFDIQDIGCRFYTYSATMGLTLEAAGENGKKYFVLDRVNPITGT
                     IIDGPVRMGKGTFIAHHEVPLRYAMTTGELARMYNREHRCNTDLTVIQVEGWQREAWF
                     DQTGLPWTNPSPNMRTLNQAILYPGIGLLESALSVGRGTDTPFEIVGAPYINDRQLAE
                     ELNGAGLAGVRFVPVQFTPNYSVHKDKLCKGVHILLTDREHCRVVDVGLVIAQTLYRL
                     YPRDFNPDKISHLLVHPPTLEAVKANKPLDEIHALWQKDLDQFLQRRAQYLIY"
     gene            16259..17584
                     /locus_tag="PKI07_01490"
     CDS             16259..17584
                     /locus_tag="PKI07_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC49752.1"
                     /translation="MRPDNQVTRREFIASTSMLLAASQLGMAQTTNREPDALALEGGV
                     KAVPRALPRPKRWGEPELQQLSAAVKQDSLYYWRNQQTQLFTERFQAIYGHKYVQPCS
                     SGSAALHIAVAAAGIAPGDEVITSGITDIGTVIGVLFQQAVPVFADLEPQTGCLDPAD
                     VARKITPRTKAIITVHLAGNPSRLAELKEIADQHKLILIEDCAQAWGARYRGQPISRV
                     GQISCFSLQDSKHIGCGDGGVVTTSHEQFGPLLIKFGDKGTNRLNPKDSSTALGANYR
                     ITELQMAFAAAQITRLENIAAQRAKLGNLLTAELAGVPGILTHKVHPEDRCVYWFYLA
                     RLVPEKLRCDRATFVKALAAEGAPFSAGYISVPLYGLPMFQNHSFFAGRWPARECGLT
                     QMDYTRVRCPETEAILKTCVRFGVNEAMTEDDIRAVAKAVRKVAKHYAA"
     gene            17612..18652
                     /locus_tag="PKI07_01495"
     CDS             17612..18652
                     /locus_tag="PKI07_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOC49753.1"
                     /translation="MHTACSRFLSAAALFCALGLAGLGAPPGAAPAGAAPVKLIFDTD
                     IGNDVDDVLALSVIHALQSRGECELLAVTITKPDELAGPFVDAVNRFYRRPGIPIGHT
                     RAALTNSPSKFLPLAEVKDGRKLRYPRRLKRSSDAPEATQLLRRVLSRQPDRSVVLVQ
                     VGYFSNLAALLDTPGDAASPLNGRDLVAQKVKLLSVMAGSFRTTHHDREYNVIQDLPA
                     AKRLVPRWPTPVIWSGFEIGIAVPYPAASIERDYRYTPHHPAAEAYYRYNPPPHERPT
                     WDLTSVLYAVRPDRGYFDLSAPGQVTVEADGFTRFNPTPGGRDRYLILTPAQAVRVKE
                     ALVQLASQPPDK"
     gene            18794..21250
                     /locus_tag="PKI07_01500"
     CDS             18794..21250
                     /locus_tag="PKI07_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49754.1"
                     /translation="MTGQAMKPGWPTQAMKNWICRRLDWGLAMGMLLLALGCVRPAAS
                     QESQTTAIPAPDTNATEAQASLEQGDELANGDEVADLAEAEATPIPADRPAPAGLKLS
                     APAAEVIKLAESGTEEGVLLAFVTNSSAPFNLGADEIIYMNDIGVSSEVVAAMIQRDQ
                     ALGGGPASVEPAPAAPVAEEPELYAPQPAESSAVAEAPPAAPPPPAETVSYPVFYNSL
                     SPYGTWIDVAGWGPCWRPTVVVANPGWRPYCDGGRWIYSDCGWYWLSDYTWGWAPFHY
                     GRWFRHHRMGWCWAPDTVWGPSWVSWRHTATHCGWAPLPPGASFTYGHGLAYRGRTVS
                     SSFGFGLGVSSFTFVETTHFHSRHLNRHVLPPTHARHVFHQAPPSTPIVMNHGRVMNH
                     GIPPARITQATRTEIHTVTLQPTRHAPGRNTRGERLDGNQRTLAVFRPEFPASTGTSR
                     GPGAPALRSPPREGGSLRTGFHEGSRAPVAASGAPSVLPERKPPSVRSSAPAGSSLST
                     PSPIGTPRPDASFTHSSSSRTLPAPNSAAPSRNPDPKPAPPLSSSPGDRPLRNVPASS
                     GSTVSREAPRPAATFSGRNESRPTTPVIPPASSLNQAVRSLAVPSQRDPLPHSATTPR
                     TPRVTTSSGSTSARTIPPPSALRSGDSPARSTPSRSGTVPSSPTPAPTRPSTPTVTAP
                     RSTPAPPPAIVRSAPAPSFTPAPARPASPPATPFTARTPSMESRPTRSTPAPTFTPQM
                     SRPASPPAAASVSRAPATVSRPTQSAPAQTFTPQPARSFSPPPTSSGQRAPAVQSQPS
                     RPASSSSSWQSPSSSARRGR"
     gene            21950..22612
                     /gene="ruvC"
                     /locus_tag="PKI07_01505"
     CDS             21950..22612
                     /gene="ruvC"
                     /locus_tag="PKI07_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOC49755.1"
                     /translation="MTAFLLCFCPSTDSGNMARMGITPQQFRQMQERVGRPRRAARAM
                     PEGMAPARVKVGQVILGLDPSLRGTGYGVIKLARPFPQTLAQGVIACPASWARSRCLV
                     EIAQTLRAVLRRHPPAVCAVEGLFYAQNLRTALIMGEARGAALAVVAEAGLEIYELAP
                     RKVKQAIVGYGAAQKLAVAKMVQRMLCLPETPAPDAADALALALALAAAPAGRFQAAP
                     RV"
     gene            22609..23214
                     /gene="ruvA"
                     /locus_tag="PKI07_01510"
     CDS             22609..23214
                     /gene="ruvA"
                     /locus_tag="PKI07_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOC49756.1"
                     /translation="MIAFLRGQLIEALPTQVTVEVNGVGYEALIPLSSYDHLPAPGQE
                     VKLLTHLVVREDAHVLYGFMTAAERELFRLLINTVSGIGPKIALNVLSGMNPTAFRGA
                     VANGDVKSLSQISGVGRKTAERIVVELKDKIGAAGAWEAASAQRALSPADQRVNDAVL
                     ALMALGFKQIEAHDSVRKAQAALGAQATVEDLVRSCLKKGA"
     gene            23244..>23584
                     /locus_tag="PKI07_01515"
     CDS             23244..>23584
                     /locus_tag="PKI07_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49757.1"
                     /translation="MKPAGGGRRRRSGGVVVPNAPRWHQRLGAWLIFALVRAVSATVR
                     YRLTDRSAYYDHGPAGPAIYCVWHNRLALCLTSYYRYVKPRSRAPGLAALVSASRDGA
                     LLAAILECFGV"
     assembly_gap    23585..23684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23685..24103
                     /locus_tag="PKI07_01520"
     CDS             <23685..24103
                     /locus_tag="PKI07_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOC49758.1"
                     /translation="ASRDGALLAAILECFGVQPVRGSSSRRGPQALLELSGWAERGYD
                     LAITPDGPRGPCYVVQEGVIGLAQVTGLPIVPASCRLGRKVRLRSWDGFQVPLPFSRC
                     DVVIGAPVRVPRDAAEAEREALRQGLERTLRELGGD"
     gene            complement(24133..25215)
                     /locus_tag="PKI07_01525"
     CDS             complement(24133..25215)
                     /locus_tag="PKI07_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOC49759.1"
                     /translation="MTLLFSVILVALIFEYINGFHDTANSIATVVATKVLTPRQAVLL
                     AAVTNLLGALWGTAVAQTISSGLVDTRIVLLSPDIMICALLGAILWNLLTWWLGLPSS
                     SSHALIGGLCGAALAASDNRWQVIVWSQHNPQHWYQGKGLLWKVIVPMFTSPLAGLLL
                     GFLVMALLYRLLRHARPGRVNRFFGKAQMLSAGAMGFMHGTNDAQKTMGIIALSLLGA
                     TTAGQLDNLPGWLSFLHTPPPPPGADMRIAWWIKVVCALTMAAGTAAGGWRIIKTLGH
                     KMVKLHPIHGFAAETSSAAVILTATHFGIPLSTTHNISAAIMGVGAAKSLNAIKWTVV
                     ESMAWAWLLTLPISGALAYGLVRLLR"
     gene            complement(25247..26326)
                     /locus_tag="PKI07_01530"
     CDS             complement(25247..26326)
                     /locus_tag="PKI07_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOC49760.1"
                     /translation="MTLLLTVIVVALVFEYINGFHDTANSVAAVVGTRALTPSRAIML
                     AAVTDLCGALAGTAVATTVGQGLVDAHCVTSVTLVCGLGGAILWNLLTWRLGLPSSSS
                     HALIGGLVGSAIASAGGDWGVVIWSAPVAGKPWYAWGGLLYKVIIPMFSSPLLGFILG
                     FFLMSLLYALLARSRPSVTNRVFSKLQILSAAFLGFSHGCNDAQKTMGIIALALFSAT
                     RMDELSHLPGWLQFLRTPEFTVPLWVKITCALTLAAGTATGGRRIIKTLGRKVSRLLP
                     VNGFTADTTSASVLLSAAVMGMPVSTTHVVSTAIMGVGAARNYKAIRWHIVESIIWTW
                     FLTLPATGLVAYGLMRLFQLFGAVT"
     gene            complement(26333..26962)
                     /locus_tag="PKI07_01535"
     CDS             complement(26333..26962)
                     /locus_tag="PKI07_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pit accessory protein"
                     /protein_id="HOC49761.1"
                     /translation="MFSLQKLFGKEDKFFKLLEASAEAARASVAALVQLSQTLDQPQA
                     FDEFAYTRKKDKEITNQISNAVYTTFITTLEREDIEELSSALYKIPKMVDKFTTRVWV
                     APQYVRGVDFTTQIQLLQQATDVVLELVRSLRHGMDLDKVKNLNDQLQALESQADSHM
                     MTLYRELFSGQREALQVIALKDLYELLEKGIDRCRDAGNVIAHIALKHS"
     gene            27102..28457
                     /locus_tag="PKI07_01540"
     CDS             27102..28457
                     /locus_tag="PKI07_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC49762.1"
                     /translation="MSAHATDLPAPNAVSRRAFLRLAGAAGLGAPFLASDLIARPPSR
                     LLGHASLGAGGMAWEDIQQLVRTGQVRLEAVAEVDLKRAAAVRKAFPQARIYQDWRKL
                     LDREARHIDSVSVSTPDHMHAPMAMSALQLGKNVYCQKPLTHDLWEARKLAEYAGARR
                     VVTQMGIQIHATSFYRMAATLARAGAIGRIKEVHCWNSGSWGDAAARPDTVDPVPEEF
                     DWDLWLGVCARRPFIGKEYYHPFYWRRRLDFGTGTLGDMGCHLLDPVFMALGLGAPNS
                     VRSEGVPPNAWNWPVDAQVHYVFPGTAYTADPTLPLTWYDGARKPPPEVRALLEGDDL
                     PGTGSLWVGTQGVLVLPHVARPMLYPDAKFKDLKLPEITEKNHWGEYVDACRGQGRTT
                     AGFNYSGPLTEAVLLGTIALRFPQATLHWDSRRLAFAEPGANPFIRRAYRAGWSVRGL
                     G"
     gene            complement(28646..30175)
                     /locus_tag="PKI07_01545"
     CDS             complement(28646..30175)
                     /locus_tag="PKI07_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOC49763.1"
                     /translation="MKKTIAHINIRSLALALGATALVAGAIAAAPKATAPAPRLEVDE
                     TPVARAAGPHISFAPIVKKVTPAVVKIVVTAKASQNSPPGWFGFDDPFWRRFFGDRFG
                     NPSPNPAPGRQFRIPRQFGLGSGVIITKDGYILTNNHVVDGADEVKVTLQDRRELTAK
                     VIGRDPKSDIAVIKVEAQDLPFVPMASSDKVEVGDLVLAIGNPFGVGQTVTTGIVSAT
                     GRGNLGIEEYEDFIQTDAAINPGNSGGALVDVEGRLIGINTAIYSRSGGNQGIGFAIP
                     SNLARNVMDSLVKYGHVSRGYLGIMIQDVTPSLAKAFKLPDASGALIGDVVSGGPADK
                     AGLKNGDVVIEYNGKKVRDSRHLKLTVGETKPGSRVPVKILRDGVNKNLEVTVRDLPD
                     DPKLAQSSRSSDADNGTLNGVTVSDLDARARRQFDIPESVKGVIVTDLEPDSAAAEAG
                     LKPGDVIQEINRTPVKTAEEAVRLTERPRSKVTMLRVWSNGGSRYMVVDESKSAREDN
                     G"
     gene            30319..30684
                     /locus_tag="PKI07_01550"
     CDS             30319..30684
                     /locus_tag="PKI07_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49764.1"
                     /translation="MENNEIIGLVPWPVAAATAVWFGVMAYKSGKNFVLWAIGGAVLG
                     LVLTTLVMGLGQATFIPYHTGEIAIFRIKLAAVAVIIVVCAGWLFTGSLHRRLSSAWK
                     KKAAAPEAGARPPAATPKP"
     gene            complement(30778..32073)
                     /locus_tag="PKI07_01555"
     CDS             complement(30778..32073)
                     /locus_tag="PKI07_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC49765.1"
                     /translation="MRILLLKPRWLVKDGVYRFLTHISFEPLHLGILAALSEGHDVTI
                     VDHDWDEIPNDRDFDLVGITATTFSSQRAFDLADGFRQKGAKVVIGGVHACLMPEECL
                     QHADAIVIGEAEYVWPQLLKDAATGALKPTYQQSQPTVMDDVPIPRRDLFRPNPLVGM
                     IQATRGCHHTCKFCYLPHVPWHQHRRRSPDKVYEELKGMKQSIVFFVDDNLFVDEDYA
                     LALCEKIAPLKKAWSVQAPTTITKNPRLLRAMQKSGCFFVQVGFQTVHPDSLARAGVV
                     QNRVETYREVVRRFHQHGILVQGFFIFGFDNEDSRIFPTAETCIKQMGLEDALLYILT
                     PYPGTPIYDQLKQQGRLLAFDREKFGWANAVFQPARMTPQELERGVQSTYENLHSFFK
                     WQAPRRILNPKWLPFFLRHPRILWVVLQALNRRVKILRD"
     gene            complement(32076..33269)
                     /locus_tag="PKI07_01560"
     CDS             complement(32076..33269)
                     /locus_tag="PKI07_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC49766.1"
                     /translation="MRRHAFFLCYFETTRRCDQGCPHCMTDPAEPASRPELSTVEAQR
                     LVLDELRELCPKGAVSFSGGEILQRADHLELIAHNARNGLYTFVNTSGSGLDSARMRK
                     LKQAAAGRLTMGFSLDSVDDDIQKKCRAGDRERLERLLHLCEEQNVPAFVLLTVSRRN
                     LASLSRTMDWLRERKIAVIRSPFVPRGAAANARHLCFDRADMGDTIHPALRANPLSYV
                     SYTPFFAAPDATRLSFGGRGLSLGNLGCQAGRTFIGINAEGDVAPCVHLLDSPVCCGN
                     VRDQALSRIVAESEIMRVLRGERPARGKCARCRYGDSCRGCRALAYYHTGDYLAEDPT
                     CFFQPDSPATRSPLEETQTANTRLFLNYLAQNRPWRDIFGVHGRLGVTLLNFKAWLNT
                     FLGSL"
     gene            33503..34069
                     /locus_tag="PKI07_01565"
     CDS             33503..34069
                     /locus_tag="PKI07_01565"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOC49767.1"
                     /translation="MLLVIDNYDSFTYNLVQYLGELAVELQVHRNDQISLEQVRALTP
                     ERILISPGPCSPREAGLSNDIIRTFGPQIPTLGVCLGHQCIGHVFGGNVVVNYRMMHG
                     KTSLIQHNGRDLFAGMPNPFAATRYHSLVIERGSLPDCLEITAQTAEGEIMGVRHKQH
                     PIWGVQFHPESILTQNGRQMLKNFLSLK"
     gene            complement(34225..34518)
                     /locus_tag="PKI07_01570"
     CDS             complement(34225..34518)
                     /locus_tag="PKI07_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOC49768.1"
                     /translation="MKLFVGNLSFNATDQQLRDLFGAHGTVQEVDIIKDKFSGRPRGF
                     GFVTMETKEGGEAAIKALHKKEVDGRELTVNEARPREERPPRTGGGYGRRREE"
     gene            complement(34644..35621)
                     /locus_tag="PKI07_01575"
     CDS             complement(34644..35621)
                     /locus_tag="PKI07_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC49769.1"
                     /translation="MRWLAFSMAGLLGCATLRGADLRIGIIGCDTSHVTAFTETINNP
                     QAPGHVSGGKVVAAYKGGSPDIPSSIEHVEGYSKTLQEKYGVRIYDRIEELCQNVDVV
                     LLESVDGRPHLEQVRPVLKARKPVYIDKPIATSLRDAREIFRLARKAKVPVFSSSGLR
                     FAKNTQAVRNGLIGRVEYAETYGPCSIEPHHPDLFWYGIHGVESLFTVMGTGCQTVQR
                     GTTTNGLIEVVGTWRGGRKGIYREDKSFRGLARGEKGEMAVGSFDGYQPLVAAIMKFF
                     QTGVAPVSPKETLEIIAFMEAADQSKRQDGAPVKIRLRPPATPARISPK"
     gene            complement(35727..36788)
                     /locus_tag="PKI07_01580"
     CDS             complement(35727..36788)
                     /locus_tag="PKI07_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC49770.1"
                     /translation="MKTLTPLAHGIRLPRLLAGVLLLALAPAGRAQPDPNWIDHDRNR
                     PQPPVVSPATASTQEQPGKPPSDATVLFDGKDLAQWVSMDGSPTKWITRDGYMECVKG
                     SGYIRTLQNFGDCQLHVEWAAPLPAEGQGQGRGNSGVFLGLNRYEIQVLDSYQNTTYA
                     DGGAGSIYGQYPPLVNACRPPGQWQTYDILYTAPRFDAEGKLLSPARLTVFHNGVLIQ
                     NNVELTGPTDWIDRAPYQPHPEKQPIALQDHGNPVRFRNIWVRELGRPGRKELTLSPA
                     LLDSYAGKYDPLEISREGSQLVARLAGRKLLLFAESPTRFFAKTTDVQIEFPGGGQGK
                     PDRLIWSVGEGAHEAKRTQ"
     gene            complement(36870..38045)
                     /locus_tag="PKI07_01585"
     CDS             complement(36870..38045)
                     /locus_tag="PKI07_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOC49771.1"
                     /translation="MNDIEVIIALLLLFMAVPDVCRRLGRPALAFPAFVVFGIVLGPL
                     ASAGVETMLHGAGVVGFLLLLFQVGLEIDLPQPRELRAAASFAVPWALVQYPVVLMLT
                     TVAGLGLTESLLAAAALTGVSVGMSYPAWKNYPGLAEGERGRILRILIVLEAMTIILL
                     AVGAPALEAGLSWFMLLRLGGIALTLLLVARFARHVRQLFQVILERTTLWRTHLLVLL
                     VLVVCAAGERLGLAAAKTAFFLGLFMSRTEHEGKGLEEYAAPVSERFLIPIFFVSLGL
                     RVEWATVGSLTGLSALGAAGLLVGVRQVLHRRWLRVGGDQRAYLLLCPNLTVVALGTS
                     RLMESPTTAAAASWLLLTGLFTTVFAIASLPATRKDSGNRSGALSPEAAKAKPLIPA"
     gene            complement(38155..39507)
                     /locus_tag="PKI07_01590"
     CDS             complement(38155..39507)
                     /locus_tag="PKI07_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOC49772.1"
                     /translation="MNRLRLLRRLALLLFICPLPALAAKDTYYAVGAAQVDITPDYAI
                     WLNGYAARQNESEGVDQRIYAQALAIGRGKNNLALLLTVDNVGVPAAVREEVAARLAR
                     KAGLRSERFVLCSTHAHTAPMLSNSIPNIFGRDLPPAEQERINRYTRELINKLEQAAL
                     AALKARQPATLAWGQTRAGFAANRRTAGGPVDHDVPVLVARDKSGRIRALLASYACHC
                     TTLGHTPNRICGDWAGYAREYLERDHPGAVVLVAIGCGADANPQPRVGLELAQQHGRT
                     LVDAINPLLQQPLKPLHQKLSCQARAIALPFDTLPSRAEWMTRAQSNNQYIAYHAKKN
                     LARLDRGEPLPTQLPYLVQTWNFGRELGMVFLTGEVVVDYSLRLKRECDPSRLWVIAY
                     ANDVPCYIPSKRIWHEGGYEGGYAMIYFDRPTRLAEGTEELIISTTHELLPREFDAAR
                     "
     gene            complement(39504..40553)
                     /locus_tag="PKI07_01595"
     CDS             complement(39504..40553)
                     /locus_tag="PKI07_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC49773.1"
                     /translation="MKKTSSRTPHRKVNVAVVGLGFMGVTHIKVYQKLKTARIVAVCD
                     SARAPVNGVLPGVAGNITGSDAIDLGRQLRVYRTLEELLADPEVELVDLCVPTALHPQ
                     QAIAALKAGKHVLCEKPLALTSAKARSMVRAARAARGFFMPAMCMRFWPGWAFLKQVV
                     TEKTYGEIQAASFRRLSATPGWSKATYSSGSLTGGALLDLHIHDTDFVQFLFGRPDSV
                     ASRGVTRGGNSIDHVVTQYHYHRGPVVYAEGTWLMAGGFNMAYTVLCERATIDYDLAR
                     GAEALRITETGKAPRVVKCDGSDGYTEEISYMLQAILSGKAPRVVTAQDGLSAIQICE
                     AEAKSVRTGRIVSLS"
     gene            complement(40567..41052)
                     /locus_tag="PKI07_01600"
     CDS             complement(40567..41052)
                     /locus_tag="PKI07_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49774.1"
                     /translation="MQISKSFLIACAAAYGATALPLYAEDTEAQVRARVALEQKLNEL
                     QSQPQPAEAPAPAPETPPPTRRQAKSPKIASPKAEKAKAPAPTPAPDAPAESAAAPAA
                     ARKPAPARAASALPQIEGPGAAISAEKQQRLDELLTQYRADQITPEEYHQLRAKILAE
                     P"
     gene            complement(41063..42115)
                     /locus_tag="PKI07_01605"
     CDS             complement(41063..42115)
                     /locus_tag="PKI07_01605"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOC49775.1"
                     /translation="MAPARPELPARLLRDVSRSFYLTLRVLPGGIRQPIGVAYLLART
                     TDTIADTEWVAPAQRLGALRALRARIQGRGPEAAPLDLGILAQRQGAPAERLLLERGE
                     ASLALLRQLPRADQQLVGEVLDTITSGQELDLQRFAGASTGALTALRTDAELEDYTYR
                     VAGCVGEFWTRICRAHLFPRAALDDAFLLANGARFGQGLQLVNILRDVAEDLRRGRCY
                     VPAERLAAAGLKPEDLLRPDSEPRWRPLYHAYLDRAEDRLRAGWAYTNALPRRSARVR
                     LACAWPILIGRETLAQLRVGNALDPARRIKISRRQVRRLLLRSVLFYPWPGAWKKLVP
                     AGKPGGKPVASPAVLP"
     gene            complement(42167..43570)
                     /gene="argH"
                     /locus_tag="PKI07_01610"
     CDS             complement(42167..43570)
                     /gene="argH"
                     /locus_tag="PKI07_01610"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOC49776.1"
                     /translation="MNKQTSPVSRSGRFAGGPAAAVAQFTESISFDRRLWRQDIAGSE
                     AHAQMLCQAGCLTRRELQAIRRGLAAIRREIAAGKFRWQPELEDVHMNLEAALTRRTP
                     AGAKLHTGRSRNDQVALDLRLWLREQIAALAGEIRQLQRALVRLAQRERTVVIPGYTH
                     LQRAQPVYLAHHLLAYVEMLARDAQRLQECQARTNVCPLGSGALAGTTLPLNRERVAK
                     LLGFVDARGRPRVTQNSMDAVSDRDFAVEFCSVAALLGVHLSRLAEDLILWSSREFNF
                     IRIADAFTTGSSLMPQKKNPDVAELIRGKSGRLIGNLVALLTLLKGLPMTYNRDLQED
                     KERLFDTTDTVRAAARLCAAMLAHTQVNRPACRAAASDPTLLATDLADYLVRRGLPFR
                     KAHHAVGALVALAERLGKPLDQLSLAELRSVEQKFGADAREVFDLPRALARRSLTGTP
                     GTREVARQLARWRKLTG"
     gene            43674..44552
                     /locus_tag="PKI07_01615"
     CDS             43674..44552
                     /locus_tag="PKI07_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49777.1"
                     /translation="MSEFKFACPVCGQHITADASASGGQLECPTCFRKIIVPSAPATR
                     DSKLLVSAAQVASPRPIPAEMAAAAGSPPTPPRRRALPALGALFLLLAAAGAAWFFLR
                     RPAPQPVPPAPPVAPHPPAPDHPVILNTNYPVPTHIQWTLALANAAFPTTPAAGRIHG
                     SGFRCERAILRKDLLSLSQGKTWPWDLGVNLNLHVRRVEELSGRSIEITPDRPRAPHI
                     LLRWKDAQQQPATETIANGYALKLEFGPMADGRIPGKIYLCLPDDAQSFVAGTFDAEI
                     RAPPPPPSRPPGQPKS"
     gene            complement(44561..45265)
                     /locus_tag="PKI07_01620"
     CDS             complement(44561..45265)
                     /locus_tag="PKI07_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49778.1"
                     /translation="MPSAPPVSTVPPPAAPASPPPAKWRGCLFYGCGTLAVLAGVIVA
                     SILITLWWVRRPLKPVELSPPEKAAVEQKLQRLGNRPAPAVDPAASAEARAPQERRYE
                     PGARTLRLTEREINGLLNANTDLGRSVRLELAQDAIQAYLAVRLPPDFPVGGGQVLRA
                     RGRFRLSLGHAGEPCAMLEDVTVLGLSLPNAWLGGLKGQNLLGQALGERPDAPLLRGL
                     KSLRIEPGALTLEVED"
     gene            complement(45298..46590)
                     /gene="clpX"
                     /locus_tag="PKI07_01625"
     CDS             complement(45298..46590)
                     /gene="clpX"
                     /locus_tag="PKI07_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOC49779.1"
                     /translation="MARPTHLTLCSFCGKSHTEVNRLIAGPGVYICDSCVLLCNQVLG
                     KEAAAPARKPKPRSHIPKPAEIKRRLDQSCIGQEHAKKTLAVAVHNHYKRLLQEPAAA
                     ASAEAGPARGDAKAEVEIEKSNILMIGPTGSGKTLLARTLAQVLDVPFAIADATTLTE
                     AGYVGEDVENIILRLLQNADYDVKRAQRGIVYIDEIDKIARKTENVSITRDVSGEGVQ
                     QALLKILEGTVCSVAPQGGRKHPQQEYIRVDTSDILFICGGAFIGLERIIQRRLGRQV
                     LGFRANPELPKAVAAERQEILQHLEPEDLLSYGFIPEFVGRLPMLTVLAELTEAQLVS
                     ILTDTKNALTKQYAKLLAMEGVELEFSPEGLRELAAQAIKKGTGARALRSLLERLMLD
                     IMYEVPGSEDILHVHITAPVVAGQCKPIIRRKQNRAAA"
     gene            complement(46599..47240)
                     /locus_tag="PKI07_01630"
     CDS             complement(46599..47240)
                     /locus_tag="PKI07_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOC49780.1"
                     /translation="MDNFLVPMVVEQTGRGERGYDIYSRLLVDRIVFLGTPVDDMVAN
                     LIIAQLLFLQMNDPKKDIHLYINSPGGSVTAGLAVYDTMQFLTCDVNTYCIGQAASMG
                     AVLLCGGTKGKRYALPNSNIMIHQLLGGAEGPASDVEIRVRYMLKLKQRLNRIIARHT
                     GHSIEQVEKDTDRDNFMTPEEAKAYGLVDEVVQSRKEIPGLAERATSLADKKE"
     gene            complement(47349..48752)
                     /locus_tag="PKI07_01635"
     CDS             complement(47349..48752)
                     /locus_tag="PKI07_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC49781.1"
                     /translation="MAEKSLSELPRELRLLFTKGNDALQRENLDYAIALFNQVLAKEP
                     ALFECRQALRSAQSRKASGGRGFFKKIMSSAGSSPQLARAHLALGKDPAEALRIAEQI
                     LNGDPANSAAHKLVVEAANALDLPKTAVMSLEILVAHSPNDQDIAVKYAHALANAGEV
                     GKAEAVLANLYRAAPHDQELAQALKNLSARKTLDEGGYEALADGTGSYRDILKDKEEA
                     VLLEQQNRQVKTEDVAERLIQEYEGRLAAEPGNLKLLRQLAELYTQKQQFDRALSYYD
                     RIKASEVGADASLDRNIADTVMRRYEHQMAQLDATAPDYADKLAALQAEKQAYQLAEC
                     QKRAERFPTDLQIRFELGQLYFQAGRITEAMREFQKAQSNPHRRIQALSYLGQCFARR
                     GIHDLAATTFQDALKEKVVFDEEKKELIYLLGSVYETMGKRDDAIAQFKIIYAADVGY
                     KDVADKIDAYYDGAAGG"
     gene            complement(49726..50295)
                     /locus_tag="PKI07_01640"
     CDS             complement(49726..50295)
                     /locus_tag="PKI07_01640"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOC49782.1"
                     /translation="MTNKSLGAKTLIYPAPVLVVGTYDKAGKPNVMTASWGGICSSQP
                     PCVAVSLRKATYTYANLLEHQAFTLSIPSEKHVKEADYFGLMTGRNTNKFAAAKLTPV
                     RSTLVDAPYVKEFPLVLECKLLHVVELGLHTQFVGEVIDAKAHASIIGTGGAVDIRKL
                     KPLIFTPDTQDYFGVGKFVAKVFSAGKDI"
BASE COUNT         8451 a        16095 c        16771 g         8926 t
ORIGIN      
        1 atcaaatcct agaaaaagtg ggtcactgca aagctaagac tgttacacca cactaggaag
       61 ggcgggccag gacggcggcc aggtattggc cggtatggct ggcgggacat tgagcgatga
      121 cttccggcgg gccctcggcg acgatttgcc cgccggcttc gccgccctcg gggccgaggt
      181 cgagcaccca atccgccgtc ttgatgacgt ccaggttatg ctcgatgacg atcaacgtat
      241 tgcccgcggc gcggagtttg aagaggactt caagcagctt ggccacgtcg tggaagtgca
      301 ggccggtggt gggttcatcc agaatgtaga gcgtgcggcc ggtggccttg cggctgagtt
      361 cggcggccag tttgacacgc tgcgcttcgc cgccgctcag ggtggtcgcc gcctggccga
      421 ggccgatgta gccgaggccg acttccgcca gcgtgagcag cggctcatag atttgcggca
      481 cggcgcggaa gaaggtcgcg gcttcatcca cggtcatatc cagcacgtcc gcgatgtttt
      541 tgcctttgta attgatttcg agcgtttcgc ggttgtagcg gcggccgttg cagccttcgc
      601 aggtgacgta caccggcggc aggaaatgca tttcgatctt gagcaagccg tcgccctggc
      661 atttctcgca gcggccgccc ttgacgttaa agctgaagcg gccggcgccg tagccccgga
      721 tgcgggcgtc cggcaggcgg gcgaagaggt ggcggatgtg attgaacatg ccggtgtagg
      781 tggccgggtt gctgcgcggg gtgcggccaa tcggcgtctg gtcaatgacg atcactttgt
      841 cgaggttttc aaagccgcgg agcgcgcggt gggcgccggg gcgttccttg gacccgaaga
      901 atttgcggcc cagcgcgcgc cgcagggtat cgtccaccag cgtgctcttg cccgagccgc
      961 tgacgccggt gatgcaggtg agcgtgccga gcgggatgcg cgcgttgagg tttttgaggt
     1021 tgttcgcgcg cgcgccgagc acttcaagcc agccgcgggc cgcggacggc tggacgcggc
     1081 ggcgcggaac cgggatggaa agctcgccgc gcaggtattt ggcggtgagt gaatgcgggt
     1141 gggcgagcac ttcggccagg gtgcccgcgg cgaccagttc cccgccgcgc accccggcgc
     1201 cggggccaag gtcgagcagg taatcggcgt gccggatggt gtcggcgtca tgctcgacca
     1261 ccagcaccga attgccgagg tcgcgcaagc cggccagggt gcgcagcagg cggtcattgt
     1321 cgcgctgatg caggccgatg ctcggctcat cgaggatgta gagcacgccg acaagtcccg
     1381 cgccgatctg ggtggccagg cggatgcgct gcgcctcgcc gccgctgagc gtgccgcttt
     1441 cgcgatccag ggtgaggtag cccagcccca cgtttcgcag gaagcccagc cgggcgcgaa
     1501 tctctttgat cagttcctgg gcgatctttt cctgaaactc cgacagcttg agcgtcgcga
     1561 agaactcgtc ggcctgctcg accgaaagcg tgcagacgtc catgatggag aggccgggcg
     1621 gagagacgcg cggggaggga ggccggggga cggcttccga ttcgcgcccg gtttcccggc
     1681 ccgccggctt gaacttcgcg ctcgcctccg catcccccag cgtcaccgcc aggatttccg
     1741 gcttgagccg ttggccctgg caggcatcgc agaactgcgg gctcatgaag cctttgagcc
     1801 ggttgcgggt gaactcgctc tcgctttcct ggtaaagccg ctggaggttg gggatgacgc
     1861 cttcgaacgg gcgggtcact ttgctggtgg cgcccatgcg ccggaagagg aactcgattt
     1921 gcgtttcgcc cgagccccac agcagcgtgc gttggaagtc ctccgggagc gtttggtagg
     1981 gggcatccat gctttgctgg taatgtttgg ccaccccttg cagcaggctt ttgtagtaaa
     2041 gcaccatccg tttgccgccc cgccgccagg gctggattgc tccctgttcg agcgacttct
     2101 ccggatcggg caccaccagc ccctcatcaa acacgagctt ctggcccagg ccgtggcaaa
     2161 ccgggcacgc ccccgccggg gcgttaaacg agaaatgctt gggggtaagc ggctcgtagc
     2221 ttttgcccgt cgcggggctg aggttgcggt tggagagaag ggtttcaacc cattccgaat
     2281 tggcgggcgg ggcgggcgag gccgcgcgcg ggccgtgagg cgcgggccgc gattcggccc
     2341 tggccgtccg gtcttccggc ggcgtttggt gcagcgtcag cagggttccc tcgccccagc
     2401 gcagcgcggt ctccacggaa tcgctcagcc ggacgcgaat cttctcatca atgaccagcc
     2461 gatccaccac cgcctcgatg gtatgccggg ctttggggtc cagtttgagg cgcaccccgc
     2521 cgcccagttc gaccagttga ccgtccacgc gggcgcggac aaagccctcg cgcgccagcc
     2581 gcgccacgac gtcgcggaac tcgcctttct gatcccgaac caccggcgcc aggagcatca
     2641 cgcgcgtgcg cgggggcagg gcgaggattt tatccacgat gtcgctggtg gtctgccgca
     2701 ggatgggcac gccggtttcc gggcaatacg gctggccgac atgcgcgtag agcaggcgca
     2761 ggtaatcata aacctcggtg gtggtggcga tgatggagcg cgggctggag ccggagctgc
     2821 gctgctcgat ggcgatcgcc ggcgacaggc cctcgatgaa gtccacgtcc ggcttctgca
     2881 tctggtccag gaactgccgg gcgtaggcgg agagcgactc cacatatttg cgctggcctt
     2941 cggcgtagat ggtatcgaat gccagggaag atttgcccga gccgctcaag ccggtgatga
     3001 ccaccagctt gtcgcgcggg atgctcagcg tgaggttctt tagattgtgc tcacgcgccc
     3061 cgccaattcg gatgaacgct ttgctcacag attgcgattc ggaccattag acattacccc
     3121 ggggcgcaag gcaactgtcc gcacccgtgg tttttccggg aggccggttc attcgccagc
     3181 cgctggcccg gccagcggca tcgccggccg tcccgggacg agcggggccg gcgcgccggg
     3241 cgtggcgctg ccgttcgggg gctccggccc gcccggcccg cgcccggcgc gcgcgcgggc
     3301 cgctacaacc gcaaatactc cggcctgatt tcgtccggct ggcagtgcag gatggcggcg
     3361 ctgcgctcga ggccgaagcg cggcttgccg gcgtagcccg ggttgaagaa gagcgttccg
     3421 cccaggcgct ggcaaaaggg cttgtgcgtg tggccaaaca ccaccacgtc cggccgctcg
     3481 cgggcaagcc gggcgcgcag gggctcggcg ggcgcctgcg gtttgacaat gtgctggagg
     3541 agaaacttgc ggccttccag gagggcgatc gccgtttccg ggtagcccaa cccgggatca
     3601 tcggtattgc cccccaccgc ggtcactggc gcgatttgct ccagctccag caggacgatc
     3661 ggcaggccga tatcgccgcc gtggagaata tgatccacat ccgcaaagag gcgcgggatg
     3721 cgggggtcga ggtagttgtg cgtatcggaa agaacgccga tcttcataga gcagggtgcg
     3781 gggcgcgcgg ggggaggcgc atttcgtgag gctggcgctc gggaaagggg ccgcgccggt
     3841 ttccgctgcc gcccggccgg tgattggccg cttaagccgg cgcttcctcc ggggcctcct
     3901 ccccctccgg ttcctcctcc ggcgccggct ccttttccga cgccggctcg ggcgcttcgg
     3961 tgagtttgag cgcgccggcg aaaagcgccg tgaacaggcc cccgctcatc agggtgttgc
     4021 ggaagaactc gaaggtgggc gggtagccgc tcgtcccttt ggtcagcgcg aggatccagc
     4081 cggccaggtc tttggggtat tccggattgg acagccagga agcggtgttg gtgagcaggt
     4141 agaagaggat cgcgccgagc aggccgcccg ccagcagccc caggaaccgc gagcggtgat
     4201 tgaaccgggt gccgagcaga atgatcagcg catacgcggc gtagttgacc aactgcagcg
     4261 cgctgacggt ctggtagtag aaattaaggg caatatccgt ggccaccagc gtgccgagcg
     4321 gcagccacca gcggacgcgg cgcgggaaga acgcgccggc gcaaaaggcc aggccgtaga
     4381 acgcgctgaa gttgggaggg aacagccccg gccagcgcga caacgccgca atcagcagaa
     4441 agagataagg cagccaggaa tgccactttc ggttgggttc acccggcata ggacggagga
     4501 cgcaaatagc ggattaaccg gcagcgttct acaggagcgc cgcttttttt tccggcaacg
     4561 ggacgatggg gggacggccg cgtttgcctt tctgccaggg ccggctgccg gaccgttcct
     4621 gctggcgtat ccggtcggca atatgacacg catcgcacaa aatagcgtag tgggttcgct
     4681 cggcgccaca gcgggcgcag cggttcagcg cgatttgctt ttgttgccag cggtactggc
     4741 gtgaaggttt tttcttcatt cgtgcattca aactcgcaaa aatagctgtt ttagtcaagt
     4801 gtctaatgca aaaaagactc gccctccggc ccgccggccg gagcgggcgg gggcctagac
     4861 atcgcagatc tgaatggcct gcttcaggga agccagcggg tcgggccgcc gggggacgaa
     4921 ctcctgtccc acgaagccct tgtagcccgt gtccacgatg gcctgcatga tggccgggta
     4981 gtagatttcc tgggtgttat caatctcgtt gcggccgggg acgccgcccg tatggtagtg
     5041 tccgatatac ggatggctct cccggatggt atggataatg tcgccctcca taatctgcat
     5101 gtggaaaata tcgtagagca gcttgagccg ctcggagccg acctgccggc agagttccac
     5161 cccccaggcg gtatgatcgg ccatgtaatc cttatggctg tgcttgctgt tgagcagctc
     5221 gaggcagacc gtcacccggt gcttctcggc aatcggcatc agccgtttca gccccttggc
     5281 gcaattcgcc aggccttccg cgtcgctcat ccctttgcga ttcccggaga aggtgatgat
     5341 attggcgaag ccggcctggg ctactttggg gatggccacc tcgaacttgg ccagcaggtc
     5401 gtcatggtgt tccaggcgat tccagccgta agggatgctg tccgggccgt tgcacatggc
     5461 gcaggtcagg ccggcctttt tcacggcggg ccattgatct tcgttgagca attccaccga
     5521 ctcgagcccc agccgctggc aggcgcggca gaagtcctcg aaggtcatgg acgccggctt
     5581 gtccttgccg gcgcggaaaa ggctggcata gcaccaggcg gagacggagt gccggatgcg
     5641 gcgcttgagc gccggcgggg cgggttcctc cgcggcggcc cccggggagg gcaggcgggt
     5701 gagcgccgcc agggcggcgg tgccttccgt caaccggcgc agcgcggcgc ggcgcgataa
     5761 gttcctgaac ataagccaaa ccttacaccc gcgggcgtcc cggagcgagg aaatactgcg
     5821 gttgcgccgg cgcgttcacg ggctcaatcg caccttgaga ctcagcgttt ccccggcggc
     5881 aagcggaacg gtgaacccca ccacgctgat ccccgggttg ggctgatcgt agtcccgcgg
     5941 cggcgggttg gccggccagg actcgagggt ggcccgggcg ggggaaataa tctcgatgcg
     6001 aagccgcttc ccgtccttct ccagccacgc gcggctgggg ccgtcgggtt tcagttgggc
     6061 cggcgtgacc attccccagc gcaccttcac aggctgggcg ccgccggtga gctggtcttc
     6121 gatcaccacc cggcggtccg gttgcagcgt gaagcggcgg ctggcgcggg ccacttgccc
     6181 ggcgtaaatc gcgctcaggt ccaccacggc atacgcgttt tccgggtcag ccgaaaaggc
     6241 cgtgatgggc gccgtgttgg tgacgacctg gtccgcgtcg tcaatgagca gcgtgctatg
     6301 cccgcggttg tggtagcgga agagggtcca gcgatcggag ccgggctgga aatcccagag
     6361 ccgaatgccg cgctgttcca gggtgttata gtcttgcgcg cccaggtcca ccgaccagcg
     6421 cacgccgttg gcatccagca caaacgagcc aatatccagg tgcgcgtggc tggccgccgg
     6481 cgtgccggcc ttgatggcca gaaacaccgc gtcgcggtcc gtccaggaac tgcggaatac
     6541 cgccagcggg ttttggccct gtcccagcca gtttcgggcg gccggttgcc ggggtttcat
     6601 cccggctttg gcccacaaca gcaccagcgg aaagtagcgc ccgccagact ggcggccatg
     6661 actgctccgg atggacgcca gcttctgctc cagcaactcg ttttcaaacc acaacagttc
     6721 cggccgttcc gtgcgcgccg caaaccacat catggccggc gaaaagccgg tgcccgagcc
     6781 gcagtcgctg aaattaaagg tccgccgcgt ggggccgatg atgttcaaca tgaactcgcc
     6841 cgtttggaga aagcccgggc tgcggctgag gccgaaatcg gtgccgaaca ccgattcaag
     6901 catcgagagg agcatgacgt tgaaactcgt gccgtagttc caatagcccg ggccttcggg
     6961 atatatccca tccgggacat acgctttcag ggcataaggc aggccggcaa gagcgcggtg
     7021 gacgacttcc gccgcctggt cgggatgatc ttccagcagc gccagggcgc cggccaccat
     7081 cccggcatgg caaacctggt tccagttgtt ccgcccgtgc tcccagcccc gccgggtttc
     7141 ggattcgaga tacggagcca atcccttttg ctcgatcgcc gtgcggctgg cctgccgcaa
     7201 ctcgggagcc aactggtcga acagccagtc atagcccacg gcgagggcca gcgtcatttc
     7261 cgccgtatcc agaaaatggg acgggttcca atccggcatg tccgccatgg ccttcatttc
     7321 ggccagcgcc cggtccgcat agcgttggtc gccggtcagg cggaaggcaa accccaggtg
     7381 catgatgcgg tttagcgcct cctgcgagcg ccccagcagc cgccggccgg ttttgcgata
     7441 aaccacgggg gaggacggca gcatggcttc cgccgtaatg cgcaccgcct ctccgatggc
     7501 ggcgagctgc gggtcggcgt tcattttttc ccgcacgccg gcttcatccc gggccgtcca
     7561 aaagagccgg ggatgcttgg gcggcggcgt cacttttttc aggatatcct gacgcgaagg
     7621 cggttcgtcc cccaacccgg caaaggttgc ggccagtaat gctcccaaaa caatcagccg
     7681 gtgcgccgag accggcagta cgccagagac cagtttcata tttaagccca tacgggtact
     7741 ttgattcggt ttcgccattt agaccagcac tatccgccgg attccagaaa tcgaggcgcc
     7801 gtcagagcca aaataatgca aaggcattag cccgattctc ctaaacccgc tccgaactgt
     7861 ccaattattg gacactttcc gtcctccggc ttcaactgtt attccgatct ccatttttcg
     7921 cctaacctcc atcatttcag gcatattcat ggctgccccc tggccgtgta caaggggtgt
     7981 acaagggatg ctcatcggat gtccaagctt aaaccctatg tacacccggt gagcatccct
     8041 tgtacacccc ttgtacatag ccaggaatga gggggagagg acgttatagg gaggttagta
     8101 gcggttaggg attgcgggat tcgactccgg tctggccgtg gggttgaggg ggaagttagg
     8161 cgggaagcct ggcgggttcc ggagcgaagg cagtgcctat ttcgagcttg cgtttccggc
     8221 gttgaatcgg aacactccct cgcctgtatg gaatctactg ttgagcaatt gccgatcctg
     8281 cacacacttt gggcctggtt tgacgcaaac aagaagccgg tcatcgtggg tacggcgatc
     8341 gttggggtcg ccgccctgat tatttccttt gtggtttatc atcggaacgc gaccgaggtg
     8401 gccgcgagcg aggcgttgtc gaaagtgccg atggagcata tgcgggcgac cggcggcaac
     8461 accatcagcg ccgatgccta tttgaaggta gccgcaacct atccgaagag cgccgccgcc
     8521 gggcgggcgt tgttgcaggc ggcgggcatt ctgttcacgg aaggcaagta tcccgaggcc
     8581 aaggcgcagt tcgagcgatt ccgccgcgag catgcgggca gtccgtttat ggggcaggcg
     8641 ctgttgggga ttgcgtcgtg cctgaatgcc cagggccaaa gccgcgaggc catgacggct
     8701 tacaaggagt tgattgatcg ccgtccggga gacagcactt tggcgcaggc ccggttcgcg
     8761 ctggcgcgct tgcatgaggc gcagaatgag ccggacaagg cgcgctccct gtacgaggag
     8821 gttgagcgca cggatgccta cggttccctg ggcgacgaat cccggatgcg gctggaggat
     8881 ctgcggcgga gccatccgaa tctcttcgcg ccggcggccg cggcggggtc gaacagcgcg
     8941 ccgttcaagg tggtcgcgcc ccaatgaacc tgtccattat cggcacgggt tacgtggggt
     9001 tggtgaccgg ggcctgtttt gcggaggtgg gtcaccgggt ggtgtgtgtg gataatgatg
     9061 cggccaaggt gcggacgctg caggcgggcg gcattcccat ttacgagccg ggcctggagg
     9121 aactggtcgc gcgcaaccgg gacgccgggc gcctgacttt taccacccac accgcggaag
     9181 gcgtggagca gtccgacgtg gtattcatcg cggtccccac gccgccgctg ccggatgggt
     9241 cggtggactt gagctacctc gaaaaggtgg cccgcgaaat tgcggcggcg atgacccgct
     9301 acaagatcgt cgtggataaa agcaccgtgc ccgtcaagac tggcgacaag gtggccgaga
     9361 ccatcaaacg ctatggcaag gcgaaggtgg actttgatgt ggtcagcaac ccggagttcc
     9421 tgcgcgaggg gtttgcggtc gaggatttga tgcggcccga tcgcatcgtg atcggggtgc
     9481 gctcgccgcg gcccgtggcg gcgctcaaac agatttacgc cccgttcgac gctcccatca
     9541 tcgtcacgga catcaactcg gcggagttga tcaagcacgc ctccaactcc ttcctggcgc
     9601 tcaagatttc ctacgtgaac gccctgtcgg tggtttgcga ggcgaccggc gccaacatcc
     9661 aggaggtggc caacggcatg ggcatggacg cgcgcattgg ccggcgattt ctgcaggcct
     9721 cgctggggtt tggcggaagc tgtttcccga aggacctcag cgcctttatc aagatcgccg
     9781 agcaggtcgg ctacgacttc cgcctgttga aggaagttca gcggatcaac gccgaccaga
     9841 tggagcggtt cgtgaagaag atcacggaca ccctctgggt gctcaaggac aagaccattg
     9901 gcgtgctggg gctggccttc aaacagaaca ccgatgatat ccgcatgtcg cccgccctcg
     9961 aattgtgcca ccgcctgcaa aaggagggcg cggccctgcg cgtgcatgat cccaaagcca
    10021 tggaaaaggc ccgggccgtg ctcaaggacg tcactttcgt cgcggacatg aacgcggtgg
    10081 ccgagggctg cgatgcgctc gtgattgcca ccgagtggga ggagttcaag caactggatc
    10141 tggagcgcgt ccgccgctcg ctcacgcatc ccatcctgtt tgacggccgc aacctgttcg
    10201 acctggcgga gatggagcgc ctgggcttca tctacaagag catcgggcgg taagccgcgg
    10261 cgcgcgggcc ggagcgggca aatgcgaaac gcgactggcc gatgaccccc agcgcgccag
    10321 ccggcgggcg gagttcgccg ggcgttcccg cgtcttgcgc gccggccgtc gaggtggccg
    10381 ccgcgctggt cttccgcgac ggccggctgc tcatcacgca gcgcccggcg gcggcgcacc
    10441 agggcgggtt gtgggagttc cccgggggca agcgcgaggc cggcgagagc ttcgaggact
    10501 gcctggcgcg cgagttgcgc gaggagctgg gcatcgaggt ggcggtcgcg gaatccgtcg
    10561 cgagcctgac gcatgcctac ccggataaga ccgtgcacct gcggttctac cgctgccgct
    10621 ggctgcggca cgagccgcgg ccgctgggct gcgcggcgtt tgcctgggtc acggcgtccg
    10681 aactcagccg ctacgccttt cccgccgctg atgcccggct gctggcgaag ctgcgggacg
    10741 aggccgggct ctggcgaacg ccgtaggccg gccggcgccc gcctcccggc ttccgccctc
    10801 cgtccccggc ggcggcgggg ccgcggcgcg gcgtttacgt tttcccgtag aactgctcga
    10861 cgacctggcg gaagttgttc tcggccacgt tgacgatgcc catctcgcgc cgggcgtttt
    10921 cctccgagtc gctggcgtga gcggcgttga ccatgatggt ttgcccgaac tcgcggcgga
    10981 tggagccggg cggcgctttg gacggatcgg tcgggccgag cacgtcgcgg atcttggcca
    11041 ccgctcccac accttcgtaa accagggcga tgcacttctc cgacccgggc ttctcctcct
    11101 cgccgggcgg acattcggcg ggcgagcgcc cggacatgaa ccgcacgatg ttgtcgaact
    11161 ggttatcgcc aaaagacggc cccaggaggg cgccgagctg ctcctgctgt tcgcgcgcaa
    11221 tcgcaaagcc gaactccttt tcgagggcgg cggcggcctt ggccgccacg acatccttga
    11281 gcttggtgcg cagggtttcg cgcaccgggc cgtagaactg caacgcctgg gccacgctca
    11341 tccggtggac tttgaccccc acgatataca agccggtgcg ggagaagaaa tcaatcatgt
    11401 tgcccggccg gccggtggcg aaccggaagt tctccggctt gatcagcacc agggtttgct
    11461 gcggcttttc cccgggctgg tagacgatga catccttcaa cacgccgccg tcggtgtccg
    11521 cgtaatgcgc ccagagcttg agcttctttt ccgcctcggc cagggtgggc gcggccagca
    11581 ccgccggctc aaagtagcgc agttgatcgt gttcgtccgt gatcaggtcc ccatacgtgt
    11641 cgcgaatggt ctcccccacc cggcggtccg ggcagacatt gccgaccacc gcccgggtct
    11701 tgctgaccgc gtcctctccc tggaagagca gcaccatgac gcgccgccgc cggccggtct
    11761 tcgggtcggg ggcgaggttg cgcaatatgt agtcgtagat gagctcctga atgttccgat
    11821 cctgggggtc gtccgcggag atggtttccc gggcgtattc cttgaccagt tccgcgctgg
    11881 gcgcgaacat gcgcgccgcc accagttcgc atcccgtgcg ggtgatcagc cgggtcagaa
    11941 tgccgcccgt gcgcgatttg tggagcgaat acggtgtaat gatgacgtaa gcaagttgtt
    12001 gaggcataga gtattattcg ggggttgaag gattgtctga cgccggtgcg gccggggcgg
    12061 aggcgggcgc cgtgggcgcg gccttgccgc ccaggatctt gaacatcacg gtgccgcaaa
    12121 ccgggcaatg gcctttaatg gctttgcgtc cgttcttcat cgtttcctcg agggcatggg
    12181 cgatttcggt cttcgctttg catttgacgc agtatccttc gggcataaac gttcgctgcg
    12241 gcggcgcttc atgcgtcgcg gcaggaatgg caggcatctt aaagggttcc cgccgcctcc
    12301 gtcaacccgg aaagggccgc gccgcgacgg ccaatccagt ggcgcgggct caggccggcc
    12361 gccgatgcac cttgtggatg gccgcctggt ccgtcggctt caccagcagc tcatcaatgt
    12421 tgacgtgcgg agggcggctg gcgacccaga cgaggatttc ggcaatgtcc tcggccgtca
    12481 gcggctgcgt gccggcataa acctgttgcg cgcgggccgc atcgcctttg aagcggacaa
    12541 gggagaactc ggtctcggcc atgccgggat ccacggagca gacgcggatg ccggtgccgt
    12601 tgagttcgag gcggagggcg cgggtaatct ggcgctcgcc ggctttggcg gcgcagtagg
    12661 cggcgccgcc ttcgtaggcg acatggccgg ccagggagcc gacgttaatg atggtgctgc
    12721 cggggttgcg ctccatcagc ggcagcgcgg cgcgggtcat gcgcagaatg cccaggacgt
    12781 taatttgcaa catggcctcc cagtcttcat ccttgccctg ggcgacgggg tcaagcccgt
    12841 gggcgccgcc ggcgttgttg atgagcacat cgagccgggc gtcggcgggc tggcgcgggg
    12901 agatccggga gcgcgcccag ttgacaaagg cctccacgct ggcggtttgc cggacatcca
    12961 gcacgtgggc gtgcgcctcc ggcgcgcccg cttggcgggc ttccgccgcc acctgttgca
    13021 ggcgttcaag ccggcgcgcg ccgagcagca accgggcgcc gtgggccgca aacgcgcgcc
    13081 ccgccgcggc cccaaaaccg ctggaagcgc cggtgattag aatccattta tctttaagcg
    13141 ggaacataaa aagcagcggg aatgttccgg ccacgcggat gccgggcccg gagccgggcg
    13201 cgggccggga agtgcgggga gccggggccg ggtcataaca cgttaatgcg ggctcgtgta
    13261 ccaggcccag atggcgatca gcagcaccgg cgccaggccg cccgccgcgc cgccgagcaa
    13321 ttccgcctgg taggagccga agcgttgccc gcaccggggc cccaggaagc ccccgaggaa
    13381 ccagccggcc aggccgcaga tcagaaagca cagaaagcca agaatgggga agcggaaaac
    13441 cagggtgagg ttatgcgcca gaatggcccg gaccccgccg atggcaaaca gccccccaat
    13501 ggagcccacg atggcgccaa tcatggcgct cagactgtat ccttccatcg ggggcttgct
    13561 catgtttcgc cgccgagttt tgcataaacg ggggagaaag gcaaagacag aatgcggttg
    13621 tcggtttgcc cgcgaccggg tttaataggc ggcctatgac aaaaccatcg cgcagcatga
    13681 atctccttct ccggtgtttt gctttttccc tgctcctgag cttgagcgcg ggccagggcg
    13741 cgccgccgcc cgcgggcgtc gcgccgtcgt ttgaccccgg gaaactggcg gaaatggatg
    13801 ccgccatcga gcaggcgatt gccgataaga aatgcccggg cggcgtttta tggctggagc
    13861 atcgcggcgc gagctatcac aaagcctatg gccggcgcgc gctggtgccc gccccggagc
    13921 cgatgtccga ggacaccatc tttgacgccg cgtcgctgac gaaagtcgtg gcctgcaccc
    13981 ccgcggtgat gctgctggtg gagcgggggc aagtgaacct ggacgcgacg gtgcagacct
    14041 atctccccga gttcaagggc cagggcaagg aagccatcac cgtgcggcaa ctgatgaccc
    14101 atacttccgg gctgcgcggc gacatcgaga cgaagaccga ttggcacggg cagcagacgg
    14161 ccatccaaaa ggcctgcgcg gagaaattgc tgagcacgcc cggcaccgcc ttttgctaca
    14221 gcgacatcaa cttcttcctg ctgggcgaaa tcgtgcagcg ggtcagccgc cagctgctgg
    14281 agaagtttgt ggcccaggag atctaccggc gcctgaagat ggcggatacg gggtttctgc
    14341 cgcccgccgc cagcctgggc cgggtggcgc ccaccgaagt ggtggcgggg cgtccctacc
    14401 ggggggtggt gcatgatccg accgcgcgca agatgggcgg cgtggccggc cacgccggcc
    14461 tgttcaccac ggcggcagac ctggcccgct atgcccggat gctcctcaac cggggttccc
    14521 tcgggggggc gcgcctcttc aaacccgaaa cggtggacct gatgacccgc gttcagacgc
    14581 ccgcgtcggt gcccgcgcgg cgggggctgg gttgggatat tgattcgggc ttcagcgggc
    14641 cgcggggaaa actcttcccc cttggctcct atggccatac cggctggacc ggcacgtcgc
    14701 tctggattga tcctttctcc cagacttttc tcatctttct gtcgaaccgc aaccacccca
    14761 gtgagagcgg caatgtgggc gccctgcggg ccaagctggg cactttggcg gcggaggcga
    14821 ttcgggattt caactttgcc tatgtgccgg gcgcgctggc ggccgcatcg ccgccggaag
    14881 cgcccgcggc gcggcgtgag gcccggccgg cccagctcaa aacgctgaac ggcattgacg
    14941 tgctcgtgcg gcagaagttt gccccgctca aggggctgcg cctcggcttg atcaccaacc
    15001 acaccggcga agaccgggat cgcaacccga ccattgacct gctcaagaac gccgaagggg
    15061 tcgagctcaa ggccctcttt tgccccgagc acggcatccg gggcacggcg gacgaaaagc
    15121 tgggggacga cgtggacgcc aagaccgggc tgccgattta cagcctttat ggccagcgct
    15181 cgaagccgac ccccgagcag ctccgggacc tggacgccct cgttttcgac atccaggaca
    15241 tcggctgccg cttctacacc tactccgcca ccatgggcct gacgctggaa gcggccggcg
    15301 agaacggcaa gaagtatttc gtgctggacc gcgtcaatcc cattaccggc accatcattg
    15361 acggccccgt gcgcatgggg aaggggactt tcatcgccca tcatgaggtg ccgctgcgct
    15421 acgccatgac gacgggcgaa ctggcgcgca tgtacaaccg cgagcaccga tgcaacaccg
    15481 acctcaccgt catccaggtc gaaggctggc agcgcgaggc gtggttcgat cagacgggcc
    15541 tgccctggac caatccctcc cccaatatgc gcaccctcaa ccaggcgatc ctctaccccg
    15601 gcatcggcct gctggaaagc gccctgtcgg tggggcgggg gaccgatacg ccctttgaaa
    15661 tcgtgggcgc gccctacatt aacgatcgcc aactggcgga ggaactgaac ggcgccggcc
    15721 tggccggcgt gcgcttcgtg cccgtccagt ttacgcccaa ttacagcgta cacaaagaca
    15781 agctctgcaa gggcgttcat attctcctga ccgatcggga gcattgccgc gttgtggacg
    15841 tggggctggt aatcgcccaa accctctacc ggctctatcc ccgggatttc aatccggaca
    15901 aaatcagcca cctgctggtg catccgccca cgctggaggc cgtcaaggcc aacaagcccc
    15961 tcgacgaaat ccacgccctg tggcagaaag acctcgatca attcctccag cggcgcgcgc
    16021 aatacctgat ttattgaaat ccaccgcccg gcgccatccg ccacccaaac ctgcggccgg
    16081 gcggcgcgcc gctggctgac cgcgccgggg acgctccggc ttccgtccgc tggcccggcg
    16141 gcgtccgccg ggcggctccg ggataacggt gggcagatcc ccggcggatc ccgggcgctg
    16201 ccgggccgcc gactttcggc ttggccccgg cgcgggaggt tttacactgg gccgcactat
    16261 gcgacctgac aatcaagtga cccgccgcga gtttattgcc tccacttcca tgttgctggc
    16321 cgccagccag ctcggcatgg cgcagacgac caaccgtgaa cccgatgccc ttgcgctcga
    16381 gggcggcgtc aaggccgtcc cccgggcgct gccccggccc aagcgctggg gcgagccgga
    16441 gctgcagcag ctcagcgccg ccgtcaagca ggactccctt tactactggc gaaaccagca
    16501 gacgcagttg ttcacggagc gattccaagc catttacggg cataaatacg tgcagccctg
    16561 ctcctcgggc agcgcggcgc tgcatatcgc ggtggccgcc gccggcattg cgcccggcga
    16621 cgaggtcatc acctcgggca tcacggatat cggaaccgtc atcggcgtcc tctttcaaca
    16681 agccgtgccc gtcttcgccg acctcgaacc gcagaccggc tgtctcgacc cggcggacgt
    16741 cgcgcgcaaa atcacccccc gcacgaaggc catcatcacc gtgcatcttg ccggcaaccc
    16801 gagccgcctg gccgagttga aagagatcgc cgatcaacac aagctgatcc tgatcgaaga
    16861 ttgcgcccag gcgtggggcg cgcgctaccg cggccagccg atttcccgcg tgggccagat
    16921 cagttgcttc tcgctgcagg attccaagca tatcggctgc ggcgatggcg gggtggtgac
    16981 gaccagccac gagcaattcg gcccgctgct gatcaagttc ggcgacaagg gaaccaaccg
    17041 gctgaacccc aaagactcca gcacggcgct gggcgccaac taccgcatca cggagctgca
    17101 aatggcgttt gcggcggccc agatcacccg gctggaaaac atcgccgcgc agcgcgccaa
    17161 actgggaaac ctgctgacgg cggagctggc gggcgtcccc ggcatcctga cgcataaagt
    17221 ccacccggag gaccgctgcg tgtactggtt ctacctggcg cgcctggtgc ccgagaagct
    17281 gcgctgcgac cgcgcgacct tcgtcaaggc gctggcggcc gagggcgctc cgttctcggc
    17341 gggctatatt tcggtcccgc tctacggcct gccgatgttc cagaaccact ccttttttgc
    17401 cgggcgctgg ccggccaggg aatgcggcct gacgcaaatg gattataccc gggtgcggtg
    17461 cccggaaacc gaggcgatcc tcaagacgtg cgtccggttc ggcgtgaacg aagccatgac
    17521 cgaagacgac attcgcgcgg tcgcaaaagc cgtccgcaag gtggcgaagc attacgcggc
    17581 ctgacccctt ccccgcccga cccctaacct tatgcacacc gcctgctccc gtttcctttc
    17641 cgccgccgcc ttgttctgcg ccctcggcct ggccgggctg ggcgctccgc ccggcgccgc
    17701 tcccgccggg gccgcgccgg tgaagcttat cttcgacacc gacatcggca atgacgtgga
    17761 cgacgtgctg gcgctgtcgg tgatccatgc gctgcagagc cgcggggagt gcgagctgct
    17821 cgccgtcacg atcaccaagc ccgacgaact ggccgggccg ttcgtggacg ccgtgaaccg
    17881 cttttaccgc cggcccggca tccccatcgg ccatacccgc gccgcgctca ccaacagccc
    17941 cagcaagttc ctcccgctgg cggaggtcaa agacggccgg aaactgcgct acccgcgccg
    18001 cctcaaacgc agctcggacg cgccggaagc cacgcagctc ctgcgccggg tcctcagccg
    18061 ccagcccgac cgctccgtcg tgctcgtcca ggtcggctac ttctccaacc tggccgccct
    18121 gctcgacacg ccgggcgacg cggcctcgcc gctgaacggg cgcgacctgg tggcgcaaaa
    18181 agtcaagctg ctctcggtca tggccggttc cttccgaacc acccatcacg accgcgaata
    18241 caacgtcatc caggacctgc ccgcggcgaa gcgcctcgtc ccgcgctggc cgacgccggt
    18301 gatctggagc ggattcgaga ttgggatcgc cgtgccgtac ccggcggcaa gcatcgagcg
    18361 cgactaccgc tacacgccgc accaccccgc ggcggaggcc tactatcgct ataacccgcc
    18421 cccgcatgaa cgccccacct gggacctgac cagcgtgctg tacgccgtcc ggccggaccg
    18481 cggctatttc gacctctcgg ctcccgggca ggtgacggtg gaggcagacg gcttcacccg
    18541 tttcaacccc acgccgggcg ggcgcgatcg ctacctgatc ctcaccccgg cccaggcggt
    18601 gcgcgtcaaa gaagccctcg ttcagctcgc cagccagccg ccggataaat agcccccggc
    18661 gccgcccccg ctcccgcacg gaaactcccg gcatgatcca aatcagacag tcccgaggcg
    18721 cgggccggcc gccccggcga agccccgccc cccggcgaat gaatacgccc cgcccggaac
    18781 gagtcaaatg cctatgaccg gccaggcaat gaagccgggc tggcccacgc aggcaatgaa
    18841 gaactggatt tgcaggagac ttgattgggg actggcgatg ggcatgctgc tgctcgcgct
    18901 cggatgcgtg cggccggcgg ccagccagga gtcgcagaca accgccatcc ccgcccccga
    18961 caccaacgcg accgaggcgc aagcctccct ggaacagggg gatgaactgg caaacggcga
    19021 cgaggtggcc gacctcgccg aggccgaggc caccccgatc cccgcggacc ggcccgcgcc
    19081 cgccggcctg aagctgagcg cccctgccgc cgaggtcatc aaactggcgg aatcaggcac
    19141 ggaggaaggc gtgctgctgg ccttcgtcac caactcgagt gctcccttca acctgggcgc
    19201 cgatgagatt atctatatga acgacatcgg cgtctcgagc gaggttgtgg ccgccatgat
    19261 tcagcgggat caggcgctcg gcggcggccc ggccagcgtc gagccggctc cagccgcgcc
    19321 ggtcgcggag gaaccggagc tgtacgcgcc gcagccggcc gagtcctccg ccgtggcgga
    19381 agccccgccc gccgcgccgc cgccgcccgc cgagacggtc agctacccgg ttttttacaa
    19441 ttccctgtcg ccgtatggca cctggataga cgtcgccggc tggggcccgt gctggcggcc
    19501 gaccgtggtc gtcgccaacc cgggctggcg tccgtactgt gatggcgggc gctggatcta
    19561 cagcgattgc gggtggtact ggctctcgga ttacacctgg ggctgggcgc ccttccatta
    19621 tggccgctgg ttccggcacc accgcatggg gtggtgctgg gcgccggaca cggtttgggg
    19681 gccatcctgg gtatcctggc ggcacacggc aacccactgc ggctgggcgc cgctgcctcc
    19741 cggagcctcg ttcacctacg gacacgggct tgcctatcgg ggccgcaccg tcagcagcag
    19801 ctttggtttt ggcctgggcg tcagctcctt cacctttgtg gaaacaaccc acttccactc
    19861 ccgccacctg aatcggcatg ttctgccgcc gacgcatgcc cgccacgtct ttcaccaggc
    19921 gccaccgtcc acgcctatcg tgatgaacca cgggcgcgtc atgaaccacg ggattccccc
    19981 cgcgcggatc acccaggcaa cgcgcacgga aattcacacg gtcaccctcc agcccacccg
    20041 ccatgccccg ggccgaaaca cccgcgggga acggctcgat ggcaatcaac ggacgctggc
    20101 ggtctttcgg cccgagtttc cggcgtcaac gggaaccagc cgcggacccg gcgcccccgc
    20161 cctccgtagc ccccctcgcg aaggcgggtc tttgcggacg ggattccacg aagggagccg
    20221 cgcgccagtg gcggcttcgg gcgcgccgtc cgtgctgccg gaacggaaac cgccgtccgt
    20281 gcggagctct gcgcccgccg ggtcgagcct ctccactcct tccccgatcg gaacgcctcg
    20341 ccccgacgcg tccttcacgc actcctccag ttcacgaacg cttcccgcgc caaacagcgc
    20401 cgccccgagc cggaatccag accccaaacc agccccgccg ctgagttcgt cccccgggga
    20461 tcggcccctg cggaatgtcc ccgccagcag cggcagcacg gtcagccggg aagctccgcg
    20521 cccggccgca actttttccg gccgaaacga atcccgcccg accacgccgg tgatcccgcc
    20581 tgcttcttcc ctgaaccagg cggtgcgatc gctggcggtc ccttcgcaac gggatcccct
    20641 gccgcactcc gcgacaaccc cgcggacgcc gcgcgtgaca acgtcgtccg ggtcaacttc
    20701 cgcccgtacc attccgcctc cgtctgcgct gcgctccggg gattcaccgg cgcggagcac
    20761 gccgtcgcgg tcggggactg tccccagctc cccaacccca gccccgacgc gccccagcac
    20821 gccaacggta actgcgccgc gcagcacgcc ggctccgccc cccgccatcg tccgcagcgc
    20881 gcccgcgccg tccttcacgc ccgccccggc tcgcccggcc tctccgccgg caacgccttt
    20941 taccgcgcgg accccgtcaa tggaatcccg tccaactcgc agcaccccgg ccccaacctt
    21001 caccccgcag atgtcgcgcc cggcttcccc gccggcagcg gcctccgttt cgcgcgcccc
    21061 ggcgacggta tcccgtccaa cccagagtgc cccggcccag accttcacgc ctcagccagc
    21121 acgctcattt tcgccgccac caacgtcctc cggccagcgg gccccggcgg tgcaatctca
    21181 gccttcccgc ccggcgtctt cgtcgtcatc ctggcaaagc ccctcttcat cggccaggcg
    21241 gggacgataa gaaccgggcg cacttcgcca caatcaccac gaccgaactg tgccaaaatg
    21301 gcacactgat taccagttgc cgaaaacagc caagcaacgc ggcgaccctc gctgaaaagg
    21361 gcacccatta agagctggat tgaagctcgc ccctgcgaaa tcttccaccc cgcaatggca
    21421 ttccaaactg tccaattatt ggacactttc ccagatacca gctttggcaa ccctatttcg
    21481 tctcgcctca gcctccaagt cactttagtc cactttagcc cgtaaatagc tcaaaaatag
    21541 ctaacaatac gccgtgtaca aggggtgtac atcggatgct catcggatga tcatggtatt
    21601 ttccgttgtt cacccggtga gcatccgatg tacacccctt gtacacagcg tattagggcg
    21661 gggaaatcag ggagaatggc tggaaaagat ggagggaatc ggggattcgt gggttgggag
    21721 gtttggcggc tggggaggga gaaccagggg ctggttttcg tttttatggg gtgccgggag
    21781 ggcagggcga tagagcctta tggcaggagc tgaactgtgc caaattggca cagtcgggat
    21841 ggggtataga aaagtttgtt ggagcccgcg aaacccgggg agttgtggtt tggcaaagcc
    21901 gggtgggagt cagggtgcgg aggatggccg cgagggaggt ttgcggagaa tgacggcgtt
    21961 ccttttgtgc ttttgcccct cgacggacag tggcaatatg gcgcggatgg gcattacccc
    22021 acagcagttc cggcaaatgc aggagcgcgt cggccgcccc cggcgcgcgg cccgggctat
    22081 gccggagggg atggcgcccg cgcgtgtcaa ggttggccag gttattctgg gtttggatcc
    22141 gtcgctgcgc ggcacgggtt atggggttat caagctggcc cggccctttc cgcagacgct
    22201 ggcgcagggc gtgattgcct gtccggcgag ttgggcgcgt tcccgttgtc tggtggaaat
    22261 cgcgcaaacc ttgcgcgcgg tgttgcgccg gcatccgccc gcggtatgcg cggtcgaggg
    22321 attgttttac gcgcagaact tgcggacggc gctgatcatg ggggaagccc gcggcgcggc
    22381 gttggcggtg gtggccgagg cggggctgga gatttacgag ttggcgccgc gcaaggtgaa
    22441 gcaggccatc gtcggttatg gcgcggcgca gaagctggcg gtggcgaaga tggtgcaacg
    22501 gatgttatgc ctgccggaaa cgcccgcgcc ggatgcggcg gacgcgctgg cgctggcact
    22561 ggcgctggcg gcggcgccgg ccggccgttt tcaagccgct ccccgcgtat gattgctttt
    22621 cttcgaggac aactgatcga ggcgctgccc acccaggtga cggtcgaggt caatggcgtc
    22681 ggttacgaag cgctgattcc gctttcctct tatgaccacc tgccggcgcc cggccaggag
    22741 gtaaagctgc tgacgcacct ggtggtgcgc gaggacgcgc atgttctcta tggtttcatg
    22801 accgccgccg agcgcgagct gtttcgcctg ctgatcaaca ccgtcagcgg catcggcccc
    22861 aagatcgcgc tcaacgtgct gagcgggatg aatcccacgg ccttccgcgg cgcggtggcc
    22921 aacggggacg tgaagtcgct gtcgcagatt tcgggggtgg gccggaagac ggcggagcgc
    22981 atcgtggtgg agttgaagga caagatcggc gccgccgggg cctgggaggc ggccagcgcc
    23041 cagcgcgcgc tctcgccggc ggatcagcgg gtcaacgatg cggtgctcgc cctgatggca
    23101 ctggggttca agcaaatcga ggcgcacgat tccgtccgca aggcgcaggc ggcgctgggc
    23161 gcccaggcga ccgtcgagga tctcgtccgg tcctgcctga aaaagggagc gtgatggttg
    23221 cggccagccg tgcatccccg ccggtgaagc cagcgggggg agggcgccgg cgccggagcg
    23281 gcggcgtggt ggtgccgaat gcgccgcgct ggcatcagcg gctgggggcg tggctgatct
    23341 ttgcgctggt gcgcgcggtt tcggcgacgg tgcgctaccg gctgacggat cgctccgcgt
    23401 attatgatca tggcccggcc gggccggcca tctattgcgt ctggcataat cgcctggcgc
    23461 tgtgcctgac gtcttattac cgctacgtga agccgcgcag ccgcgcgccg gggctggccg
    23521 cgctggtcag cgccagccgg gacggcgcgt tgctggccgc cattctcgag tgcttcggcg
    23581 tgcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgcca gccgggacgg
    23701 cgcgttgctg gccgccattc tcgagtgctt cggcgtgcaa ccggtgcggg ggtcttccag
    23761 ccgccgcggg ccgcaagccc tcctggaact ttccggctgg gccgagcgcg gctacgacct
    23821 ggccatcacg cccgatgggc cgcgcgggcc gtgttatgtc gtgcaggagg gggtgatcgg
    23881 gttggcgcag gtcaccggcc tgccgatcgt gccggcctcc tgtcgcctgg gccggaaggt
    23941 ccggctccgg agctgggacg gctttcaggt tcccctgccg ttctcgcgct gcgacgtggt
    24001 gattggcgcg ccggtgcgcg tgccgcgcga cgctgcggag gcggagcgcg aggcgctgcg
    24061 gcaggggctt gagcggacgc tgcgggagct ggggggggat tgagcggcgg ggccggcccg
    24121 gggcggatgt ttctaccgca gcaggcgcac gaggccgtag gccagagcgc cgctgatggg
    24181 aagggtgagc agccaggccc aggccatgct ttcgaccacg gtccatttga tggcgttcag
    24241 gcttttggcg gcgccgacgc ccatgatggc ggccgagatg ttgtgcgtgg tggagagcgg
    24301 gatgccgaag tgggttgcgg tcagaatgac ggcggcggag ctggtttcgg cggcgaagcc
    24361 gtggatgggg tggagcttca ccattttatg gcccagcgtc ttgatgatgc gccagccgcc
    24421 ggcggcggtg cccgcggcca tcgtcagcgc gcagacgacc ttaatccacc acgcgattct
    24481 catgtccgcg cccggcggcg gcggcggggt gtgcaggaag gacagccagc cgggcaggtt
    24541 gtcgagctgg ccggccgtgg tggcgccgag caggctgagg gcgatgatac ccatggtttt
    24601 ctgcgcgtcg ttggtgccgt gcatgaagcc catcgcaccc gcgctgagca tttgcgcctt
    24661 gccaaagaag cggttgaccc ggcccgggcg ggcgtggcgc agcaaccggt agagcagcgc
    24721 catgaccagg aagcccagga ggaggccggc caggggggag gtgaacatgg ggacgatgac
    24781 tttccagagc agtcccttgc cctgatacca gtgctgcggg ttgtgctggg accagacgat
    24841 gacctgccag cggttgtccg aggcggccag cgccgcgccg cacaggccgc cgatgagcgc
    24901 gtggctggag ctggagggca ggcccagcca ccaggtgagc aggttccaga ggatcgcgcc
    24961 cagcagggcg cagatcatga tgtccgggct gagcagaacg atccgggtat ccaccaggcc
    25021 cgaggagatg gtttgggcga cggccgtgcc ccagagcgcg ccgaggaggt tggtgacggc
    25081 cgccagcagc accgcctggc ggggcgtcag gactttggtg gcgacgacgg tggcaatcga
    25141 gttggcggta tcatggaagc cgttgatgta ttcgaagatc aacgcgacga ggataacgct
    25201 gaacagcagc gtcataagtt tccgcccgcg ccgggctgtg gtctgctcat gtgacggcgc
    25261 cgaagagctg gaacagccgc atcagcccat aggcgaccag gccggtggcg ggcagggtca
    25321 ggaaccaggt ccagatgatg ctttcgacga tgtgccagcg gatggccttg tagttgcgcg
    25381 ccgcgccgac gcccatgatc gccgttgaga cgacgtgcgt cgtcgagacg ggcatgccca
    25441 tcaccgcggc gctcagcagc acggaggcgg aggtggtgtc ggcggtaaag ccgttgacgg
    25501 gcaggaggcg ggagactttg cggccgaggg ttttgatgat gcgccggccg ccggtggcgg
    25561 tgcccgccgc cagggtaagg gcgcaggtga tcttcaccca gaggggcacc gtgaactcgg
    25621 gggtgcgcag gaactggagc cagccgggca ggtgggacag ttcgtccatg cgggtggcgg
    25681 agaagagggc gagcgcgatg atgcccatgg ttttttgggc gtcattgcag ccgtggctga
    25741 agccgaggaa cgcggcgctc aggatctgga gtttgctgaa cacgcggttg gtcaccgaag
    25801 gccggctgcg ggcgagcagg gcatagagca gggacatcag gaagaagccg aggatgaatc
    25861 ccaggagggg cgagctgaac atggggatga tgaccttgta gagcaggccg ccccaggcgt
    25921 accagggttt gcccgcgacc ggggccgacc agatgacgac cccccagtcg ccgccggcgg
    25981 aggcgatggc cgagccgacc agcccgccaa tcagcgcgtg actcgaactg gagggcaggc
    26041 ccagccgcca ggtcagcagg ttccagagga tcgccccgcc caggccgcat accagcgtca
    26101 cgctggtgac gcaatgcgcg tccaccaacc cttggcccac ggtggtcgcc acggccgtgc
    26161 cggccagcgc gccgcagagg tcggtgaccg ccgcgagcat gatggcgcgc gagggggtca
    26221 gggcccgggt gccgacgacg gcggccaccg agttggccgt gtcgtgaaag ccattgatgt
    26281 actcgaagac gagtgccacg acaatgactg tcagcagcaa cgtcatgacg gttcaggaat
    26341 gcttcagggc gatatgggcg atgacattgc cggcgtcgcg gcagcggtcg atcccttttt
    26401 ccagcagttc gtagaggtcc ttcaaggcga tgacttgcag ggcttcgcgc tggccgctga
    26461 agagttcccg gtagagggtc atcatgtggc tgtcggcctg gctttcgagc gcctggagct
    26521 ggtcattgag gtttttgact ttatccaggt ccatgccgtg gcgcagcgag cggaccagtt
    26581 cgagcaccac gtcggtggcc tgttgcagca attggatttg cgtggtgaaa tccacgccgc
    26641 gcacgtattg gggcgccacc cagacgcgcg tggtgaactt gtccaccatc ttggggattt
    26701 tatacagcgc gctggagagt tcctcgatgt cctcgcgttc cagcgtggtg atgaaggtgg
    26761 tatagacggc gttgctgatt tggttcgtga tttccttgtc cttcttgcgg gtgtaggcga
    26821 attcgtcgaa ggcctgcggc tgatccagcg tctggctcaa ctggaccagc gctgcgacgc
    26881 tggcgcgggc cgcctcggca ctggcttcca ggagcttaaa gaacttgtct tctttgccga
    26941 agagcttttg gagcgaaaac atacgggagg tattactacc tgagcggcgg agaagcgcac
    27001 gcaaaaaaag cagggggcaa tggcgtcgcc tcggggcgcg gcggacgtcc ggggtggttt
    27061 tccgttgacg gcgggcgggg gttctggctt gttgcgggac aatgtctgct catgccactg
    27121 atttaccggc ccccaacgcc gttagccgcc gtgcgtttct ccggctggcc ggggcggcgg
    27181 gcctgggggc gcctttcctg gcgagtgatt tgatcgcccg tccgccgtcc cgcctgctgg
    27241 gccatgccag tttgggggcg ggcggcatgg cgtgggagga tatccagcaa ctggtccgga
    27301 cggggcaggt ccggctggag gccgtggccg aggtggattt gaagcgggcg gcggcggttc
    27361 gaaaggcgtt tccgcaggcc aggatttacc aggattggcg caagttgctg gatcgcgagg
    27421 cccggcatat tgactcggtc agcgtctcca cgcccgacca catgcacgcg ccgatggcga
    27481 tgagcgcgtt gcagttgggc aaaaacgttt attgccagaa gccattgacg catgatctct
    27541 gggaggcgcg gaagctggct gaatatgccg gcgcccggcg ggtggtgacc caaatgggca
    27601 tccagattca tgcgaccagt ttttatcgca tggccgccac gctggcgcgg gcgggcgcca
    27661 ttggccggat caaggaagtc cactgctgga acagcggctc ctggggcgat gccgcggccc
    27721 ggccggacac ggtggacccg gtcccggagg agttcgattg ggatctgtgg ctcggggttt
    27781 gcgcccggcg cccgtttatc ggcaaggagt attaccatcc cttttactgg cgccggcggc
    27841 tggactttgg caccggcacg ctgggggaca tgggatgcca tttgctggac ccggtcttca
    27901 tggccctggg gctgggcgcg cccaactccg tgcgctccga gggcgtgccg cccaacgcat
    27961 ggaattggcc cgtggacgcg caggtgcatt atgtctttcc cggcaccgcc tataccgccg
    28021 accccacctt gccgctgacg tggtatgacg gggcccgcaa accgcccccg gaggtccgcg
    28081 ccttgctgga gggcgacgac ttgccgggca ccggctccct gtgggtgggg acgcagggcg
    28141 tgttggtgct gccgcatgtg gcccggccga tgctttaccc ggacgcaaag ttcaaggacc
    28201 tgaagctgcc ggagataacg gagaagaatc attggggcga atacgtggac gcctgccggg
    28261 ggcagggccg gacgaccgcc gggttcaatt attcggggcc gctgaccgag gctgtcctgc
    28321 tgggcacgat cgcactgcgt tttccgcagg ccaccctcca ttgggattcg cgccggctgg
    28381 cttttgcgga accgggagcc aacccgttta ttcggcgggc ctaccgggcc ggctggtcag
    28441 tccgggggct gggctgattc tggcgccgcc gcgcagggtt ttggccggag gccgcggggg
    28501 gtcggagggc ggggggatgt tccggcgtcg cgcggggttg atccggcgcc gcccgaaaag
    28561 gcgagagccc cgggcggggc tctcgttcac ggaggcgcaa gtgaaagcgg gcgttttgcc
    28621 cgcgggaagc tactgcggcg gttgctcagc cattatcttc gcgggctgat ttgctttcgt
    28681 ccacgaccat gtagcggctg ccgccgttgc tccagacgcg cagcattgtg actttgctgc
    28741 gggggcgctc ggtcaggcgc acggcttcct cggccgtttt gaccggagtg cggttaatct
    28801 cctggatgac gtcgccgggt ttgaggccgg cttcggctgc ggcggaatcc ggttcgaggt
    28861 cggtgacaat cacacccttt acgctttccg ggatatcaaa ttgccgtcgt gcccgagcgt
    28921 ccaggtcgct aacggtcacg ccattgaggg tgccgttgtc ggcgtcgctc gaacggctgg
    28981 attgcgcgag tttcggatcg tcgggcaagt cccggacggt cacttcgagg ttcttgttga
    29041 cgccgtcgcg caggattttc acgggcaccc gggagccggg cttggtttcg ccaacggtga
    29101 gtttaaggtg ccggctgtcg cggacttttt tgccgttgta ttcgatgacc acgtcgccgt
    29161 tcttcaggcc cgccttgtca gccgggcctc cggaaacgac gtcgccgatg agcgcgccgc
    29221 tggcatccgg gagcttgaac gctttggcca gcgacggggt gacatcctga atcatgatgc
    29281 ccaggtagcc gcggctgaca tggccgtatt tgaccaggct gtccatgacg ttgcgggcga
    29341 gattcgaggg gatggcaaag ccaatgccct ggttgccgcc ggagcggctg tagatggcgg
    29401 tattgatgcc gatgagccgg ccctccacgt ccaccagcgc gccgccggaa ttgcccgggt
    29461 tgatggccgc atcggtctgg atgaagtcct cgtattcttc gatgccgagg ttgccccggc
    29521 ccgtggcgct gacaatgccg gtggtgacgg tttggcccac gccgaaggga ttgccgatgg
    29581 ccagcacaag gtcgcccacc tcgactttat cgctgctggc cattggcaca aaaggcaggt
    29641 cttgcgcctc cactttgata acggcgatat ccgacttcgg gtcccgcccg attaccttgg
    29701 ccgtcagctc ccggcggtct tgcagcgtga ctttcacctc gtcggcgccg tccaccacgt
    29761 ggttattggt gagaatatag ccatctttgg tgataatcac accggagccc agcccgaatt
    29821 ggcgcgggat gcggaactgc cggccgggcg ccgggttggg agacgggttg ccaaagcgat
    29881 cgccaaagaa ccggcgccag aaaggatcgt cgaagccgaa ccagccggga ggggagtttt
    29941 gggaggcttt cgccgttacg acaattttaa ccaccgccgg ggtgaccttt ttgacgatgg
    30001 gcgcgaaact gatgtggggc ccggcggcgc gcgccacggg ggtttcgtcc acttccagcc
    30061 ggggggcggg cgcggtggcc ttgggcgcgg cggcgatggc ccctgcgacc agggctgtgg
    30121 cgcccagcgc cagggccaat gatcgaatgt ttatatgagc gatagtcttt ttcataagtg
    30181 ttgccatgtt attattggta ttgatatggt attggagtgt tggaagcgtg acagaaagct
    30241 ttcgcgggca ttacactttt gtaatgaagg ttgactggcc cgggcgaacg tttcactgtg
    30301 gattaaggtc tgatcagcat ggaaaacaat gagatcatag ggttggtgcc gtggcccgtg
    30361 gccgccgcga cggccgtgtg gtttggcgtg atggcataca agtcgggcaa gaactttgtg
    30421 ttgtgggcca ttggcggcgc ggtgcttggg ttggtgctca ccacgctggt catgggcctg
    30481 gggcaggcca ctttcattcc ctaccacacc ggcgagatcg cgatcttccg gattaagctg
    30541 gcggcggtgg ccgtcatcat cgtggtttgc gcgggctggc tgtttaccgg ttccctgcat
    30601 cggcggctgt ccagcgcctg gaaaaagaaa gccgcggcac cggaggcggg cgcccggccg
    30661 ccagcggcca ctcccaagcc gtagccccgg tccggcccgg ggattttgcc catgcggcct
    30721 ctggggtgcg ggggcgggag ttgcccggcg tggcgggttt tgcgccgggc gcggcggtta
    30781 atcccggagg attttgaccc ggcggttcag ggcttgcagg acgacccaga ggatgcgcgg
    30841 atgccgcagg aagaagggca gccatttggg attcagaatc cgccgcggcg cctgccattt
    30901 gaaaaaagag tgcaggtttt cgtaggttga ttgaaccccg cgctccagtt cctgcggggt
    30961 catgcgggcg ggctgaaaca ccgcgttggc ccagccgaat ttttcccggt caaaggccag
    31021 caggcggccc tgttgcttta gctggtcata gatcggcgtg ccggggtagg gggtcaggat
    31081 gtagagcagg gcgtcttcca gccccatttg cttgatgcag gtttcggcgg tggggaagat
    31141 tcgcgagtcc tcgttgtcaa agccgaaaat gaagaagccc tgcacgagga tgccatgctg
    31201 gtgaaagcgg cgcacgactt cccggtaggt ttccacccgg ttctgcacta cgcccgcccg
    31261 cgccaacgag tccgggtgca cggtttgaaa gcctacctgc acgaagaaac agccggattt
    31321 ttgcatggcg cggagcaggc gcgggttttt ggtgatcgtg gtgggcgcct gcacggacca
    31381 ggcttttttc aggggggcaa tcttttcgca aagcgccagg gcgtagtcct cgtccacgaa
    31441 caggttgtcg tccacgaaga atacgatgga ttgcttcatg cctttgagtt cttcgtaaac
    31501 tttgtcgggg gaacggcggc gatgctggtg ccaggggacg tggggcagat agcagaactt
    31561 gcaggtatgg tggcagccgc gggtggcctg gatcattccg accaggggat tgggccggaa
    31621 gagatcccgc cgcgggatgg gcacatcgtc cattaccgtg ggctgacttt gctggtaagt
    31681 gggcttcaga gcgccggtgg cggcatcttt aagcagttgg ggccagacgt attcggcctc
    31741 gccgatgaca atggcgtcgg cgtgctggag gcattcctcc ggcatcaggc aggcgtgcac
    31801 gccgccgatg acgactttgg cgcccttctg gcggaatcca tccgcgaggt caaaggctct
    31861 ttgcgaggag aaagtggtgg cggtgattcc caccaggtcg aagtcccggt cgttggggat
    31921 ttcgtcccag tcatggtcca cgatcgtcac gtcgtgtccc tccgaaaggg cggccaaaat
    31981 accgaggtgc agcggctcga aggaaatatg cgtcaggaag cggtacacgc cgtccttgac
    32041 gagccagcgc ggcttgagca ggagaatccg catgactaca ggctccccag gaaggtattg
    32101 agccaggctt taaagttgag cagcgtgacg cccagccggc cgtggacgcc aaaaatgtcg
    32161 cgccagggac gattttgggc caggtaattc aggaagaggc gcgtgttggc cgtctgtgtt
    32221 tcttcaagcg ggctgcgggt cgcgggggaa tcgggctgaa agaagcaggt ggggtcttcg
    32281 gccaggtagt cgcccgtgtg gtaataggcc agggcgcggc agccccggca ggaatcgcca
    32341 tagcggcagc gcgcgcactt gcctcgggcc ggacgctcgc cgcgcagcac acgcatgatt
    32401 tcggactcgg caacgatgcg ggacaaggcc tgatcgcgca cattgccgca gcaaaccggg
    32461 ctgtccagca ggtgcacgca gggggccaca tccccttccg cgttgatccc gatgaaggtg
    32521 cgccccgcct ggcagcccag gttgcccagg ctcagtccgc ggccgccgaa gctcaggcgg
    32581 gtcgcatccg gcgcggcgaa gaatggggtg tagctgacat agctcagcgg attggcgcgc
    32641 agggcgggat gaatcgtgtc tcccatatca gcccggtcaa agcataagtg ccgggcgttg
    32701 gccgccgcgc cgcgcggcac aaagggcgag cggatgaccg caattttccg ctcccgcagc
    32761 caatccatcg tgcgcgacaa cgacgccagg ttgcgccggg agactgtcag cagcacaaaa
    32821 gccggcacgt tttgctcttc gcacagatgc aggagccgct ccaggcgctc ccggtcgccc
    32881 gcgcggcatt ttttctgaat gtcatcgtcc accgaatcga gggaaaaacc catcgtcagg
    32941 cgcccggcgg cggcctgttt gagctttcgc atccgggccg aatccaggcc gctgccactg
    33001 gtattgacga aagtataaag accattgcgg gcattgtgcg cgatcagttc caagtgatcc
    33061 gcgcgctgca gaatctcgcc gccggaaaac gaaacggcgc ctttggggca aagttcccgg
    33121 agttcatcga gcaccaggcg ctgggcctcg acggtggaca gttccggccg ggaggcgggc
    33181 tccgccgggt cggtcatgca gtggggacag ccctggtcgc agcgccgggt ggtctcgaaa
    33241 taacagagga aaaacgcgtg ccttctcatc ggtttaccgc gcaaggcaac gcgaagcggg
    33301 gtcggaaact ccggtgccac ggcgttcaag gctcgcaaat cgcatgtcag gaatcatctt
    33361 accatccggt ggtcaaacgc caaggaaaag ccaaaacgcc gccgcggtgt ccgcaccgcg
    33421 ccaggcgggc catcgcccgc ccaggtttga tttcctgatt ttcaaatcgg catgcctggc
    33481 ttaaggttcc tcgcaacgca atatgctgtt ggtcatagac aactacgatt cgttcactta
    33541 caacctcgtg cagtatctgg gcgagctggc ggtggaactt caagtccacc gcaacgacca
    33601 gatttcgctg gagcaagtgc gcgctttgac cccggagcgc atcctgattt cacccgggcc
    33661 ctgctcgccg cgcgaagcgg gcctgtccaa tgacatcatc cgcacgtttg ggccgcagat
    33721 tccgacgctg ggtgtctgcc tggggcacca gtgcattggc catgtctttg gcggcaacgt
    33781 ggttgtcaac taccgcatga tgcatggcaa gacctcgctc attcaacata acggccggga
    33841 cttgtttgcc ggcatgccca atcccttcgc cgccacgcgc tatcattcac tggttatcga
    33901 gcggggctcc ctccccgact gcctggaaat aaccgcccag acggctgagg gggaaattat
    33961 gggcgtgcgg cacaagcagc acccgatctg gggcgtgcag tttcatccgg aaagcatcct
    34021 gacccaaaac gggcgccaaa tgctaaagaa ctttctgagc ctgaaatagg ttcggagagg
    34081 gtcgcccgcg ccgcccatct aaagggcggg ttggccgcgg ggattcgggg gaccagcccg
    34141 gtaaaccaaa aggcgcggga agcagttcgt tcccgcgcct tgcttcaggg cgttcctccg
    34201 aacgccttaa gtcattcgcg tttattactc ttcacgccgc cggccatagc cgccgccagt
    34261 gcgaggcggg cgctcctcgc gcgggcgtgc ttcattgacg gtcagttccc ggccgtccac
    34321 ctctttttta tggagtgcct tgatggctgc ctcgcccccc tccttggtct ccatcgtcac
    34381 gaatccgaag ccgcgaggcc ggccgctgaa cttatccttg atgatatcca cttcctggac
    34441 tgttccgtgc gcgccgaaaa ggtcgcgcag ttgttgatcg gtggcattga acgatagatt
    34501 ccccacaaac agtttcatac tcattatgat ttggcttctt gactgattgc ccttccgcga
    34561 ccgttcccga ccatcgggtt tcaaatcatc agtgaaacag gttcatctga ggatttacct
    34621 ggcggcgaaa tgggcaagtc aatctatttt ggggaaatcc tagcgggggt ggcgggggga
    34681 cgcaaccgta tttttaccgg cgcgccgtcc tgccgtttgc tctggtcggc cgcttccatg
    34741 aaggcaatga tttccagcgt ttccttgggg ctaaccgggg ccacgccggt ttggaagaac
    34801 ttcatgatcg ccgccaccag cggctggtag ccatcaaagg agcccacggc catttcccct
    34861 ttctcgcccc gggccaggcc gcggaagctc ttgtcttcac ggtagatgcc tttgcgtccc
    34921 ccacgccagg tgccaacgac ttcgatcagg ccgttggtgg ttgtgccgcg ctgaacggtt
    34981 tggcagcccg tgcccatcac cgtaaacagg ctttccaccc catgaatccc ataccagaac
    35041 aggtccggat ggtgtggttc aatcgagcag gggccgtagg tttcggcata ctcgacccgt
    35101 ccaatcagac cattgcgtac cgcctgcgtg tttttcgcaa aacgcagccc ggaggaactg
    35161 aacaccggca ccttggcctt ccgagccaac cggaaaattt cccgcgcatc ccgcagtgag
    35221 gtggcgatgg gcttgtcaat ataaaccggc ttgcgcgcct ttaataccgg ccgcacttgc
    35281 tccaggtgcg ggcgcccgtc cacgctctcc agcagcacca catctacatt ctggcataac
    35341 tcctcaatcc ggtcgtaaat cctcaccccg tacttctcct gcaatgtttt ggaataaccc
    35401 tcgacgtgtt caatgctcga cgggatgtcc gggctgccgc ctttgtaggc cgccacgact
    35461 ttgccgccgg aaacatgtcc cggggcctgc ggattattaa tcgtctcggt gaacgcggtc
    35521 acatgcgagg tgtcgcagcc aataatgcca atccgcaagt ccgcgccccg caacgtcgcg
    35581 cagcccagca gccccgccat ggaaaaagca agccatctca taccgataac agtgttgttg
    35641 tgcacaatgc cgccaaggat agggcaactg ccggccaaga ccagaacgaa atggcggggc
    35701 agttgcgtcc ccgggggacg ccgcgcttac tgagttcgct tcgcctcgtg ggcgccttcg
    35761 ccgactgacc aaatcaggcg gtcgggtttg ccctggccgc cgccggggaa ctcgatttgc
    35821 acatccgttg ttttggcgaa gaatcgggtc ggcgactcgg cgaaaagcag gagcttgcgg
    35881 ccggccagcc gcgccaccag ttggctgcct tcgcgcgaga tttcgagagg gtcatacttg
    35941 cccgcgtagc tatcgagcag cgccggggac agagtgagtt ctttgcgccc aggccggccc
    36001 agttcgcgca cccagatatt gcggaaccgc accgggttgc cgtggtcctg cagcgcgatg
    36061 ggttgttttt ccgggtgcgg ctggtagggg gcgcggtcaa tccaatcggt cgggccggtc
    36121 agttcgacgt tgttctgaat cagcacgccg ttgtggaaca ccgtcaggcg agcgggagaa
    36181 agcagcttgc cctcggcgtc gaagcgcggg gcggtgtaga ggatgtcgta agtttgccac
    36241 tggccgggcg ggcggcaggc attgaccagc gggggatatt gcccatagat ggagccggcg
    36301 ccgccgtcgg cgtaggtggt gttttggtag gaatcgagca cttggatttc atagcggttc
    36361 aagcccagga aaacgccgct gttgccgcgg ccctggccct ggccttcagc gggcagcggc
    36421 gcggcccatt cgacatgcag ttggcagtcg ccgaagtttt gcagtgtgcg gatatagccg
    36481 ctgcccttca cgcattccat gtagccgtcc cgcgttatcc atttggtcgg gctgccatcc
    36541 atgctgaccc actgcgcgag gtccttgcca tcaaacagca ccgtggcatc cgagggcggt
    36601 ttgccgggct gctcttgcgt gctcgcggtg gcggggctga ccaccggggg ctgcgggcgg
    36661 ttccgatcat ggtcaatcca attcgggtcc ggctgcgccc ggccggccgg cgcgagcgcc
    36721 agcagcagaa caccggccag gaggcggggc aggcgaatac cgtgagcaag cggcgtaagc
    36781 gttttcataa gcgaggtttg attgttcagc ttcatcagat ggggcagcgt agctttccgc
    36841 cgcgccggat ggaagccttc tttgcgccgt tacgcgggga tgagcggctt cgctttcgcg
    36901 gcctcgggac tcaacgcgcc gctgcggttg ccgctgtcct tgcgcgtggc gggcagggac
    36961 gcaatcgcaa acacggtggt aaacaacccg gtcagcaaca gccaactggc cgcggcggcc
    37021 gttgtgggac tttccatcag gcgcgaggtt cccagggcga cgaccgtcag gttgggacag
    37081 agcagcaaat aggcgcgctg atcgccgccc acgcgaagcc agcggcgatg caggacctgg
    37141 cgcaccccca ccagcagccc cgccgcgccg agcgcgctca acccggtcaa tgatcccacc
    37201 gttgcccatt ccacccgcag ccccagcgac acgaagaaga tggggattaa aaaccgctcg
    37261 cttaccggcg cggcatattc ctccaggcct ttgccctcgt gctcggtccg gctcatgaat
    37321 aatcccagaa agaaggcggt cttggccgcc gccagcccca accgctcgcc cgccgcgcat
    37381 accaccagca ccagcagcac cagcaggtgc gtccgccaga gcgtcgtgcg ctccaggatc
    37441 acctgaaaga gttggcggac atgccgcgcg aatcgtgcca ccagcagcag cgtcagcgcg
    37501 atgccgccca gacgcagcag catgaaccag cttagcccgg cttcgagcgc cggtgctccc
    37561 accgccagca gaatgatggt catggcctcc aatacgatca ggatgcgcag aatccggccg
    37621 cgctcgcctt cggccaggcc cgggtagttt ttccatgccg ggtagctcat gcccaccgag
    37681 acgccagtca acgcggcggc ggccagcagg gattccgtca ggcccaaccc cgccaccgtg
    37741 gtcagcatca gcaccacggg atactgcacc agcgcccagg gcaccgcaaa actggccgcc
    37801 gcccgcagtt cccgcggctg cggcagatca atctccagcc ccacctggaa caggagcagc
    37861 aaaaacccca ccacgcccgc cccgtgcagc atcgtctcga cgcccgcgct ggccagcggc
    37921 cccagcacga tgccaaacac gacaaaggcc ggaaacgcca acgcgggccg ccccagccgc
    37981 cggcaaacgt ccggcacggc catgaacagc agcagcaggg cgataatgac ctcaatatcg
    38041 ttcatgcgtc aacgcaatga aacccggccc cggccgggcc gggcatccgc cggcgcccgg
    38101 cctcgcccgt cggcggatta aaaatccgcg tcgccttccg cggccggcgt tcgctcaccg
    38161 ggcggcgtcg aattcccgcg gcagcaactc gtgggtagtg gagataataa gctcctcagt
    38221 cccctctgcc aggcgggtgg ggcggtcgaa gtagatcatg gcgtaaccgc cttcgtaacc
    38281 gccttcatgc cagatgcgct tcgaggggat atagcagggc acgtcattcg cgtaggcgat
    38341 cacccacagc cggctggggt cacattcccg tttcagtcgc aacgagtaat ccaccaccac
    38401 ttcgcccgtc aggaacacca tgcccagttc ccggccgaag ttccaggtct ggaccaagta
    38461 gggcagttgg gttggcagcg gctcgccacg gtcgagccgg gccaggttct ttttcgcgtg
    38521 ataggcgata tactgattat tggattgcgc gcgcgtcatc cattccgcgc gtgaaggcag
    38581 ggtgtcgaac ggcagcgcga tggccctggc ctggcaggac agcttctggt gcaggggttt
    38641 gagcggttgc tgcaagagcg ggttgattgc gtcgacgagg gtgcgcccgt gctgctgggc
    38701 cagttcgagg ccgacgcgcg gttgcggatt ggcatccgcg ccgcagccga ttgcgaccag
    38761 caccaccgcg ccgggatgat cgcgttccag gtattcgcgg gcgtagccgg cccaatcgcc
    38821 gcagatgcgg ttgggcgtgt ggcccagcgt ggtgcagtgg caggcgtagc tggccagcag
    38881 ggcgcggatc cggccggact tatcccgcgc caccagcacc ggcacatcgt ggtccaccgg
    38941 cccgccggcg gtgcggcggt tggcggcgaa gccggcccgg gtctggcccc aggccagggt
    39001 ggccggctgg cgggctttga gggcggccag cgcggcttgc tccagcttgt tgatcagctc
    39061 gcgcgtgtag cggttgatcc gctcctgctc cgccggcggc aggtcccggc caaagatgtt
    39121 cgggatcgag ttggagagca tcggcgcggt atgcgcatgg gtcgaacaca gaacgaatcg
    39181 ttcggagcgc aacccggcct tgcgggccag gcgggcggcc acttcctccc gcaccgccgc
    39241 cggcacgccg acgttgtcca cggtcagcag cagggcgagg ttatttttac cgcggccaat
    39301 ggccagggcc tgggcgtaaa tacgctggtc cacgccctcc gattcgtttt ggcgcgccgc
    39361 gtacccgttc agccagatcg cgtagtccgg cgtaatatcc acctgcgcgg cgcccacggc
    39421 gtaataggtg tctttggccg ccagcgcggg cagggggcag ataaacagca gcagcgcgag
    39481 ccgccggagg agtcttaagc ggttcatgac agggatacga tgcgtcccgt ccggacggat
    39541 ttagcttcgg cttcacaaat ctggatggcg ctcaggccgt cctgcgcggt gaccacccgg
    39601 ggggctttgc cgctcaggat tgcctgcagc atatagctga tttcctcggt gtagccatcc
    39661 gaaccatcac acttcaccac gcgcggggcc ttgccggttt cagtaattcg caaagcttcg
    39721 gctccccggg ccaggtcgta atcaatcgtc gcccgctcgc agagcaccgt ataggccatg
    39781 ttaaacccgc cggccatcag ccaggtgccc tcggcataga ccaccggccc gcggtgatag
    39841 tggtattggg tgacgacgtg atcaatggaa ttgccgccgc gcgtgacgcc gcgggacgcg
    39901 acgctgtcgg gccggccgaa aaggaactgc acgaagtcag tgtcgtgaat atgcaaatcc
    39961 aacagcgcgc cgccggtcag gcttccggag ctgtaggtcg ccttgctcca gccgggggtg
    40021 gctgacaggc gccggaagct ggcggcctgg atttcgccgt aggtcttttc cgtgaccact
    40081 tgcttgagga aggcccagcc aggccagaac cgcatgcaca tggccggcat gaagaacccg
    40141 cgggcggctc gggcggcgcg caccatgctc cgggccttcg ccgaggtgag ggccaggggt
    40201 ttttcgcaca gcacgtgctt gccggccttg agcgccgcga tggcctgctg cggatgcagg
    40261 gccgtgggca cgcacaggtc caccaattcc acttcgggat cggccagcaa ttcctcgagg
    40321 gtgcgataga cccggagttg gcggcccagg tcaatcgcgt ccgatccggt aatgttgccc
    40381 gccacgccgg gcagcacccc gttgacgggg gcgcgggcgg aatcacagac ggccacgatg
    40441 cgggccgtct tgagcttttg gtaaaccttg atatgggtca cgcccatgaa cccaaggccc
    40501 acgaccgcga cgttgacttt tcgatgggga gtccgggatg aagttttctt cataagagaa
    40561 tcagggttaa ggttcggcca ggattttggc gcgtagctgg tggtattcct cgggggtgat
    40621 ctggtcggcc cggtactggg tcaacagctc gtccaggcgc tgttgtttct ccgcggaaat
    40681 tgccgcgccg gggccttcaa tttgcggaag cgccgatgcg gcccgggcgg gagcgggttt
    40741 cctggccgcg gcgggcgcgg cggcggactc cgcgggggcg tccggtgccg gcgtcggggc
    40801 cggggccttt gctttttctg ccttcgggga agcgatcttc ggcgatttgg cctggcgccg
    40861 ggttggcggg ggagtttccg gggcgggcgc gggagcctcc gccggttgcg gttgcgactg
    40921 caattcgttg agtttttgct ccagggccac gcgggcccgg acctgcgcct ccgtatcttc
    40981 cgcgtagagc gggagcgccg tcgcgccata cgccgccgcg caggcgatca gaaaggattt
    41041 agaaatctgc atgcgggttc ttttacggca aaacggcggg cgacgcaaca ggtttcccgc
    41101 ccggttttcc ggccggcacc aactttttcc aggccccggg ccagggataa aacaggaccg
    41161 aacgcagcag gagccgccgg acctggcgcc ggctgatttt gattcgccgc gcggggtcga
    41221 gcgcattgcc aacgcgcaac tgcgcgagcg tctcccggcc aatcaggatc ggccacgcgc
    41281 aggccagccg cacgcgcgcg ctgcggcgcg gcagggcgtt ggtataagcc cagcccgccc
    41341 gcaggcggtc ttccgcgcgg tccaggtatg cgtggtagag cgggcgccag cgcggctcgc
    41401 tatccggccg caacaggtct tccggtttca atcccgcggc cgccagccgc tcggcgggga
    41461 catagcagcg cccgcggcgc aaatcctccg ccacgtcgcg caggatattt accagttgca
    41521 gcccctggcc gaatcgcgcg ccgttggcca gtagaaaggc gtcgtccagc gccgcccggg
    41581 ggaaaagatg cgcgcggcat atccgggtcc agaactcgcc gacgcagccg gccacgcggt
    41641 acgtgtaatc ctccagctcg gcgtccgtgc gcagcgccgt cagcgcgccg gtcgaagcgc
    41701 cggcaaaacg ctgcaagtcc agttcctgcc cgctggtgat ggtatccagc acctcgccga
    41761 cgagctgttg atcggcgcgc ggcagttgcc gcagcagcgc caggctcgct tcgccgcgct
    41821 ccaacagaag ccgctccgcc ggcgccccct gccgctgggc caggatgccc aaatccagcg
    41881 gcgccgcttc cgggccccgg ccctgaatcc gcgcgcgcag ggcgcgcagg gcgcccaggc
    41941 gttgcgccgg cgccacccat tcggtatcgg cgatggtatc ggtcgtgcgc gccagcaagt
    42001 aagccacccc gatgggctgc cgaatcccgc cgggcagcac ccgcagcgtc aggtaaaaag
    42061 aacgcgagac atcccgcagc aggcgggcag gcagttctgg acgcgcgggg gccatggggt
    42121 tgcggacggg agttccgggc cgccgcgagg ggcggcggcg gcccgctcag ccggttaact
    42181 ttcgccaccg cgccaactgc cgggccacct cccgggttcc cggcgttccc gtgagcgaac
    42241 gacgcgccaa agcgcgcggc aggtcgaaca cttcccgcgc gtccgccccg aacttctgtt
    42301 ccaccgagcg cagctccgcc aggctgagct gatccagcgg tttgcccagc cgttcggcca
    42361 gcgcgaccag cgcgcccacg gcgtgatgcg ccttgcggaa gggcaggccc cgccggacga
    42421 ggtaatccgc caggtccgtg gccagcagcg tgggatcgct ggccgccgcc cggcacgccg
    42481 ggcggttgac ctgggtgtgc gcgagcatgg cggcgcacag gcgcgcggcg gcccgcaccg
    42541 tgtccgtcgt gtcgaaaagc cgctctttgt cttcctgcaa atcgcgatta taggtcatcg
    42601 gcaggccctt gagcagcgtc aacaacgcga ccaggttgcc aatcagccgc ccgcttttgc
    42661 cccggatcaa ctccgccacg tcggggttct tcttctgcgg catcagggag gagccggtgg
    42721 tgaaggcatc cgcgatgcgg ataaagttga actcgcggct cgaccacagg atcagatcct
    42781 cggccagccg cgacaggtgc acccccagca aggccgccac gctgcagaat tccaccgcga
    42841 aatcccggtc gctgacggcg tccatggagt tttgcgtcac gcgcggccgg ccccgcgcat
    42901 ccacaaaccc cagcagcttc gccacccgct cgcggttcag cggcagggtg gtcccggcca
    42961 gcgcgccgct gcccaggggg caaacgttcg tccgcgcctg gcactcctgc agccgctggg
    43021 cgtcgcgcgc gagcatctcc acgtaggcca ggaggtggtg cgccaggtaa accggctggg
    43081 cccgctgcaa gtgggtgtag cccgggatga ccaccgtgcg ctcgcgctgc gccagccgca
    43141 ccagcgcgcg ctggagctgc cgaatctccc cggccagcgc cgcgatttgc tcccgcagcc
    43201 aaagccgcaa atccagcgcc acctggtcat tgcgcgagcg gcccgtatgc agcttggctc
    43261 ccgccggggt ccgccgggtc agggccgcct caaggttcat gtggacatct tccagctccg
    43321 gctgccagcg gaacttgccc gccgcgattt cccggcggat ggccgccagc ccccggcgga
    43381 tggcctgcag ctcccgccgg gtcaggcagc cggcctggca cagcatttgg gcgtgcgcct
    43441 ccgaccccgc aatgtcctgg cgccagagcc gccggtcgaa ggagatggat tccgtgaatt
    43501 gcgccacggc cgccgccggg ccgccggcga atcgtccgct ccgcgagaca ggtgaagttt
    43561 gtttgttcat gcgcttcgct cgcggttgaa tgtgcgccgc cgcggcgggg atgtcactgc
    43621 caaaaaaagc attgtccccg ccggcttcgt gtggaatcgt gggccgcgga accatgagcg
    43681 agttcaaatt cgcgtgtccg gtttgcggcc agcatatcac ggccgacgcc agcgccagcg
    43741 gcgggcagct cgagtgccca acttgctttc ggaagatcat cgttccctcg gcgccggcga
    43801 cccgggattc caaactcctc gtctcggcgg cccaggtcgc cagtccgcgc ccgatccccg
    43861 cggagatggc cgccgcagcg ggctcgccgc caacgccgcc ccgccggcgc gcccttcccg
    43921 ccctgggggc cctgtttctg ttgctggccg ccgcgggcgc agcctggttt ttcctccgcc
    43981 gcccggcgcc acaacccgtt ccgcccgctc cgccggtcgc gccccatccg cccgcgccgg
    44041 accatccggt gattctcaac accaactacc cggttccaac ccatatccaa tggacgctgg
    44101 cgctggccaa cgccgccttc ccaaccacgc ccgccgccgg gcggattcat ggcagcgggt
    44161 ttcgatgcga gcgggccatt ttgcggaagg acctgctgtc gttaagccag ggcaagacct
    44221 ggccgtggga cctgggcgtc aatctcaacc tccacgtccg gcgcgtggag gagctgagcg
    44281 ggcggagcat tgaaatcacg ccggaccggc cccgcgcgcc gcacattctg ctgcgctgga
    44341 aagatgcgca gcagcagccc gccacggaaa ccattgccaa cggctacgcc ctcaagctgg
    44401 agtttggccc gatggccgac ggacgcatcc ccggcaagat ttacctctgc ctgccggatg
    44461 acgcccagag cttcgtcgcc ggaactttcg acgcggaaat ccgagcgccc ccgcctccgc
    44521 cgtcgcgccc gccgggccag cccaaaagct gacccgcgct tcagtcctcg acttccagcg
    44581 tcagcgcgcc gggttcgatc cgcaggcttt tgaggccccg gagcaggggc gcgtccgggc
    44641 gttctcccag ggcctggcca agcagattct gacctttcaa tccccccagc cacgcgttgg
    44701 gcaggctgag ccccaggacg gtgacatcct ccagcatcgc gcaaggttcg cccgcatggc
    44761 ccagtgacag ccgaaaacgg ccgcgcgcgc gcagcacctg gccgccccct accgggaaat
    44821 ccggcggcaa ccgcacggcc aggtaggcct ggatggcgtc ttgcgccagt tccagccgca
    44881 ccgaccgccc caggtccgtg ttggcgttca ataaaccgtt gatctcccgc tccgtcagcc
    44941 gcaacgtccg ggcgcccggc tcatagcgcc gttcctgcgg cgcgcgcgcc tccgcgctcg
    45001 cggcgggatc aacggcgggc gccgggcgat tgcccaaccg ttggagcttc tgttccacag
    45061 ccgccttttc cggaggggac aactccacgg gcttcagggg ccgccgcacc caccacagcg
    45121 taatcagaat cgaggccacg atcacccccg ccagaacggc cagggtcccg catccgtaaa
    45181 acaggcagcc gcgccacttc gccggcggcg gcgaggcggg ggcggccggg ggcggaacgg
    45241 tggaaacggg aggggcggag ggcatacggg ccgcgcgcgg cgctccgggg gccggggtca
    45301 ggccgccgcg cggttctgtt tgcggcggat gatcggtttg cactggccgg cgaccaccgg
    45361 cgcggtgatg tggacgtgca ggatatcctc gctgccgggc acctcgtaca tgatgtcgag
    45421 catcaggcgt tcgagcaggc tgcgcaacgc gcgcgcgccg gtgcctttct tgatggcctg
    45481 cgccgccagc tcgcgcagcc cctcgggcga aaactccagc tccaccccct ccatcgccag
    45541 cagcttcgca tactgcttgg tcagcgcatt cttcgtgtcc gtcaaaatcg agaccaactg
    45601 cgcctccgtc aattccgcca gcacggtcag catcggcagc cggcctacga actcgggaat
    45661 gaagccgtag ctgagcaaat cttcgggctc gaggtgttgc aggatttcct gccgctccgc
    45721 cgccacggct ttcgggagct ccgggttcgc gcgaaacccc agaacctggc ggcccaggcg
    45781 gcgctggatg atccgctcca ggccaataaa ggcgccgccg caaataaaca ggatgtcgct
    45841 cgtatccacc cggatgtatt cctgctgcgg atgcttgcgg ccgccttgcg gcgcgacgct
    45901 gcagaccgtg ccctccagga tcttcagcag cgcctgctgc acgccttcgc ccgacacatc
    45961 ccgcgtgatc gagacgttct cggtcttgcg ggcgatcttg tcaatttcat caatatagac
    46021 aatgccgcgc tgcgcccgct tcacgtcata atcggcgttc tggagcaggc gcagaatgat
    46081 gttctcgacg tcctcgccca cgtagccggc ttcggtcagc gtcgtggcgt ccgcaatggc
    46141 gaacggcacg tccagcacct gcgccagcgt gcgcgcgagc agcgttttgc ccgacccggt
    46201 ggggccgatc atcaggatgt tgctcttttc gatctcgacc tcggccttcg cgtcgccccg
    46261 cgccgggccc gcctcggcgg acgccgccgc cgccggttcc tggagcaggc gcttgtaatg
    46321 attgtgcacc gcgaccgcca gcgtcttctt ggcgtgctcc tggccgatgc acgactggtc
    46381 cagccggcgt ttgatctcgg ccggtttggg gatgtgggag cgcggcttgg gcttccgggc
    46441 gggcgccgcc gcctccttgc ccaggacctg gttgcagagg aggacgcagc tatcgcaaat
    46501 ataaacgccc ggcccggcga tgagccggtt gacctccgtg tgggacttgc cgcagaagct
    46561 gcagagcgtc aggtgggtcg ggcgcgccat gcgacgggtt actccttctt gtccgccagg
    46621 ctggtggccc gttcggccag gccggggatt tccttgcgcg attgcaccac ctcgtccacc
    46681 agaccgtagg ccttggcctc ctcgggcgtc atgaaattgt cgcggtcggt gtctttctcg
    46741 acctgctcaa tcgagtggcc ggtgtgccgg gcgatgatgc ggttgaggcg ctgcttgagc
    46801 ttcagcatgt agcgcacgcg gatttccaca tcactggccg ggccttccgc cccgcccagc
    46861 aactggtgaa tcatgatgtt cgagttcggc agcgcgtagc gcttgccctt ggtgccgccg
    46921 cacaacagca cggcccccat gctcgccgcc tggccgatgc agtaagtgtt cacatcgcag
    46981 gtgaggaact gcatggtgtc atagaccgcc agcccggcgg tgacgctgcc gcccggggaa
    47041 ttgatataga gatgaatgtc cttcttgggg tcgttcatct gcaggaacag cagttgcgcg
    47101 atgatcaggt tggccaccat gtcgtccacc ggcgtgccca gaaaaacaat ccggtccacc
    47161 agcagccgcg agtaaatgtc atacccgcgc tccccgcgcc cggtttgctc caccaccatc
    47221 gggactaaaa aattatccat agcaaagcga tgtgttatac cggggcaacc taacggaagc
    47281 cccggggcgg gtcaagccgg cgccccgccg cccgccgtcg cgcccgccgc gccggggcgg
    47341 ccgggatctc agccgcccgc cgcgccatcg taataggcgt caatcttgtc ggccacatcc
    47401 ttgtagccca catccgccgc gtagatgatc ttgaactgcg cgatggcgtc gtcgcgcttg
    47461 cccatcgtct catacaccga ccccagcagg taaatcagct ccttcttttc ctcgtcgaag
    47521 accaccttct ccttgagggc gtcctggaag gtggtggcgg ccaggtcatg gatgccgcgc
    47581 cgcgcaaagc attggcccag gtagctcaac gcctggatgc gccggtgcgg gttgctttgc
    47641 gccttctgaa actcccgcat ggcctcggtg atccggcccg cctggaaata aagctgcccc
    47701 agctcgaagc ggatttgcag gtccgtgggg aagcgttcgg cgcgcttctg gcactcggcc
    47761 agttggtagg cctgcttttc ggcctggagc gccgctagtt tgtcggcgta gtccggcgcg
    47821 gtggcatcga gttgcgccat ctggtgctcg tagcggcgca tcacagtgtc ggcaatattg
    47881 cgatccagcg acgcatcggc gccaacctcg gacgccttga tccggtcgta gtagctcagc
    47941 gcgcggtcga actgctgttt ctgggtgtac agctcggcca gttggcgcag cagcttcagg
    48001 ttgcccggct cggccgccag ccgcccctcg tattcctgaa tgagccgctc ggcgacatct
    48061 tccgtcttta cctgccggtt ctgctgctcc agcaggacgg cctcctcctt gtccttgagg
    48121 atatcacggt aggagccggt gccgtcagcc agcgcttcgt agcccccttc gtccagcgtc
    48181 ttgcgggcgg aaaggttttt gagcgcctgg gccaattcct ggtcatgggg cgcggcgcgg
    48241 tagagatttg ccagcaccgc ctcggctttc cccacctcgc cggcattggc cagcgcgtgg
    48301 gcatacttga cggcaatgtc ctggtcattg ggcgaatggg cgaccaggat ttccagcgac
    48361 atcacggccg ttttaggcag gtccagggcg ttggccgcct ccaccaccag cttgtgggcg
    48421 gcggaattgg ccggatcccc attgaggatc tgctcggcga tccgcagggc ctcggccgga
    48481 tctttgccca gggccaggtg ggcccgggca agctgcggcg aggaacccgc gctgctcatg
    48541 atcttcttga aaaaaccacg gcctccgctg gctttgcggc tttgggcgct gcgcagggcc
    48601 tggcggcact cgaaaagcgc gggttctttg gctagcacct ggttgaagag ggcgattgcg
    48661 taatcaagat tctcccgctg taacgcgtcg ttgcccttgg taaagagcag gcgcagttcg
    48721 cgtggcagtt cgctgaggct cttttcagcc atagtcgagt gttgtcagat tcgtcaggat
    48781 aaatctagtg gaattatgcc gcccggcgca acccgcgttt tcccgccgcg ccgctttggg
    48841 ccgcgcctca aagccgaaaa gcatgccgct cccgttcccg gtgccgccca agaccgccgg
    48901 caaccggtgc aggccggggg gcgcggcaat aacaggcaag gcggcctcgc cagcgggaca
    48961 atcttcgccg gaaactcaga tcgggaacgg ttcaggcggg cgttttaatc atgtgccatc
    49021 gtctcgctgg agcctgggtg gccctgacag cccagaccgt aattcccttg aacccccgga
    49081 ttgatgattt caagcgcata gccgataaga gctgaacgcc tttcgcgggt aaaatcaatg
    49141 atttacgcac ccaggaagga gctgaatgga ggcgaaacct gagtcttttc ggctcaaagg
    49201 cgccttgggc gtaccttgca aataccttgg atttaccctg cttccaggcc gtgtacaagg
    49261 ggtgtacaag ggatgctcat cgggagttca tgggatttta gctggtacac ccgatgggca
    49321 tcccttgtac accccttgta cacggcctga tggcagtgga ttttcagggg atttggacaa
    49381 tggctatggg agcttggaaa ggtcgagatg ataagagagg actggaggat aattattaag
    49441 ttccttaaaa acagtcagct atgaatccag ggctggcaaa aaagggaggg caagggcggg
    49501 cgggcggtaa gcccgcggat gccctgcgag gcatggttca ccgggccgag cgccgggagc
    49561 ttgcggacgg aatcgggcgg gaactggttg ggaaatcggt gggtagcccg gtcgagcgag
    49621 gtatcggcga tgcgcttcgg ccccttccgc tcacgaaatg ggagtctggt tcgggggccc
    49681 aggccggtct cttgggcaac gggtagtggc cggaggcggc agcgatcaga tatctttgcc
    49741 ggcggagaac accttggcca caaacttgcc gacgccgaag taatcctgcg tgtccggtgt
    49801 gaagatcagc ggcttgagct tccggatgtc cacggcgccc ccggtgccga tgatgctggc
    49861 gtgcgccttg gcgtcaatca cctcgcccac aaactgcgta tggaggccca attccaccac
    49921 gtgcagcagc ttgcactcca gcaccagcgg aaactctttc acatacggcg cgtccaccag
    49981 cgtgctccgg accggcgtca gcttcgcagc cgcaaacttg tttgtgttgc gccccgtcat
    50041 caagccgaag taatccgcct ccttgacgtg cttctccgag gggatgctga gggtgaaggc
    50101 ctggtgctcc agcaggttgg cataggtgta agtggccttg cgcaaggaca ccgccacgca
    50161 cggcggctgg gaactgcaaa tgccccccca cgacgccgtc atcacattgg gtttgcccgc
    50221 cttgtcgtag gtgcccacca ccagcaccgg cgccgggtag atgagcgtct tggctccgag
    50281 agatttgttc gtcatgcccg ccatcctgcc ccggcccgcc gccgttttca agctcccaat
    50341 ccg
//
LOCUS       DAOPQG010000008        48167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_960_length_48167_cov_17.276085, whole genome shotgun sequence.
ACCESSION   DAOPQG010000008 DAOPQG010000000
VERSION     DAOPQG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 48167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48167
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_960_length_48167_cov_17.276085"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1511
                     /locus_tag="PKI07_01645"
     CDS             288..1511
                     /locus_tag="PKI07_01645"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOC49783.1"
                     /translation="MNLDTLYSELTLKTNAKLVLLVLDGVGDVATREQGYLTPLEAAA
                     TPNLNALAKDSAQGRMIPVAPGITPGSGPGHLGLFGYDPLEFQVGRGVIEALGLGLEL
                     RAGDVAARANFCSLDAKGIVTDRRAGRIDTAVCEELVALLSQKIKKVGDAEVIIKAGK
                     GHRFVVVFRGKGLEGPLSDADPHREGLPVPQAAPVNPKSVKAKKAAKLVAGFYQAALP
                     LLAQKKPANGFLMRGIAHQPEIPLFQDRYELRPACIAVYPMYKGLAQLVGMTKLEGPQ
                     TIAQQFERYLAERGNYDFFFIHFKYTDMYGEDGNFAAKRKAIEEVDAALPILLRQKPD
                     VLVVTGDHSTPCVMKGHSWHPQPVMLHSAFSGSDKLERFTDTGANAGSLGVFPAKYLI
                     RLMQANARMLDKFGA"
     gene            1511..2230
                     /locus_tag="PKI07_01650"
     CDS             1511..2230
                     /locus_tag="PKI07_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOC49784.1"
                     /translation="MARLVFDIETSALPLEQFDDAQQEYLFRDCLKMADEAAQAARRA
                     EIQQQFNLWPFTAQVVCVAMLNADSLRGQVLFTAEDFEEEADEAGPVEFMACMDEVEL
                     LTAFWDVAKHYEAIVTFNGRGFDVPFLYLRSALLNVPITRKDWLGYRFQTEPHCDLAE
                     QLTFYGVSGREGAARRFNLDFYCKAFGIESPKSQGVTGADMNALIAAGRYREIAEYCL
                     RDVKATVLLYHLWQERLAGIK"
     gene            2227..2892
                     /locus_tag="PKI07_01655"
     CDS             2227..2892
                     /locus_tag="PKI07_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49785.1"
                     /translation="MKAGGKPPGPARKEWACFAVREEARPFARRAAGRGGPRIILTGI
                     GRRNAEEVIRAALARERPARVLSCGFAGGLRPELAAGTVLFAAGAETGLEPALRAAGA
                     QPARFYCADQVAATAAAKQALRAATGADAVEMESGAISAVCREQNLPCATVRVILDTA
                     GEDLPLDFNRLMNARRQLDYGKLALALALSPGKIRELLRLRRRAEAAAEKLARVLAAI
                     TAV"
     gene            complement(2879..3313)
                     /locus_tag="PKI07_01660"
     CDS             complement(2879..3313)
                     /locus_tag="PKI07_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOC49786.1"
                     /translation="MAKLLFILLIGLVFEAAGVVLLKKGIGQIGEVKSVSAAEILRVA
                     QAGITNGNVLLGVFFEALFFACLLFLLAKSEVSFLWPLTALSFVMTTLAAILFLGERV
                     SSLRWAGVVFIVIGAALISYSEHAKPKPLRPAAAREAGATPP"
     gene            complement(3315..4163)
                     /gene="hpnK"
                     /locus_tag="PKI07_01665"
     CDS             complement(3315..4163)
                     /gene="hpnK"
                     /locus_tag="PKI07_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HOC49787.1"
                     /translation="MASSGFQPLSPPRRLIVNADDFGRSSAINQAVIRAHREGILTTA
                     SLMVGGAAFAEAVALARTHPRLGVGLHLTLVCGSAVLPRAEIPGLVDARGEFSRNPAG
                     AGFRYFFRRRLHGQLRREIHAQFAKFRATGLPLDHVNGHLHLHLHPTIFRLLMDDAAP
                     LGIQRLRLTSDPFRLNLRLASGRFFSRLGHAVIFHRLAARARPALARSGIKHPRAVFG
                     LLQNARVDEAYLQRLLPQLPAGESELYSHPSLDDFKQEFDALVSPRVREQVKRLGIEL
                     IRYQDL"
     gene            complement(4153..5019)
                     /gene="hpnD"
                     /locus_tag="PKI07_01670"
     CDS             complement(4153..5019)
                     /gene="hpnD"
                     /locus_tag="PKI07_01670"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HOC49788.1"
                     /translation="MQASRKITRKSASNLALAFVLLPAAKRNAMSALYAFCREVDDVA
                     DEESLSTAQRREQLAAWRADVRRACGTETPRLAVNRELQPFIHQYGLAFEHFDALLQG
                     VEMDLDCTRYEDYERLDLYCYRVASVVGLLSIEVFGYRNPGCRDYAVNLGKALQLTNI
                     LRDVRTDAERGRIYLPLAELARFKVAPEEILRLEYSTRFGDLAASVAGRARQFYQAAR
                     ETLPAADRRSMVAAELMGCVYGRLLRKLERRRFRVFGPAPIRLNRAHKLLLILRAWGR
                     HVAGAGPADYGV"
     gene            complement(5029..5400)
                     /locus_tag="PKI07_01675"
     CDS             complement(5029..5400)
                     /locus_tag="PKI07_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49789.1"
                     /translation="MSLPLFFLNVVTVDDVVRTWQSIRPPSPAALLMFSALAAVTLVL
                     VLWAVYLRKPRRRHSRHSWHHHASRPRPAARGPVNGPAAAAPGTPRRRRRSRRKHRRR
                     NPTLAETGGLPAIRPDIPPAP"
     gene            complement(5403..6827)
                     /locus_tag="PKI07_01680"
     CDS             complement(5403..6827)
                     /locus_tag="PKI07_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC49790.1"
                     /translation="MSSRLPPVLVVDDEKNMRLSLKTVLTDERYGVRTVESAEEALEL
                     LEREEFFMVITDVRLGGMSGYELLGKVRAQWPDLPVLMITAYATPKLAVEAIKAGAMD
                     YLAKPFAPEELLHAAARCAERYRLLRENASLRARAAESWRLDQIVGACQKMRDLRQLI
                     QTVAPTDARVLVLGESGTGKELVAGALHSLSQRSGAAYVRINCAAIPEALLESELFGH
                     EKGAFTGALKQKPGRVEEADGGTIFLDEIADMSRPLQAKLLRFLEDGSFLRVGGTAEL
                     RVNVRLIAATNRDIVEAIRQGQFREDLFHRLNVVQFRLPPLRERGEDVLLLADYFLQA
                     FNEALHKKTRAISRAARQKLQAHHWPGNVRELRNVIERALILETTPEIQAASLPDFQL
                     EGRLHKKAAPRLTGSESLDDRMADFERELITAMLEQNHYSLTRTAEQLKVSRHALRYR
                     MQRLNITTGTDAEEDSSAAPGKAP"
     gene            complement(6934..8577)
                     /locus_tag="PKI07_01685"
     CDS             complement(6934..8577)
                     /locus_tag="PKI07_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC49791.1"
                     /translation="MNLGLWQRLSHYSLRELAGMLRRLPGRAVELLKLSAPDAAKLQD
                     RIAFLERDLVLPIKAAVIAMLLYSFYFKQRWVGVKELGTLEIAVETTQYFLWVYIAAN
                     AVVGGLLLGMRRLPLLLVQWAVFAISLVDGIFLSALVVVTGGYDSILYWLFLGLIIRG
                     AVSVPRATSQLLLNLTLTGCYIMAGSVNIEINKTLEEEYLTRMAWPAPLRMARASNAP
                     PVRGLAPPPAPEGVAPPETTDQLLPVAPPLPSRHPAAQNLDESMRASLGFYASADEPT
                     QTLTLRLALLVLMTICCYGVQVLLERQRRVVEEEREFAMREGQLRSAGRVAAEFAHQM
                     KNPLAIINNAAFSLQRALTQGRPIGAEQIQIIQEEVAHSDRIITQIMGYAQLSEGHVE
                     KLAVGEELDKAIAQVFPPAAEYPVRIHRQYAGEFPPLFMQRRHLVDAVVNLLQNAREA
                     LGAAGGQITVSAVCHGDHSIEIAVRDDGPGIPPDKLEKIFEAYFTTKEKGTGLGLATV
                     KHNVELYGGSVRVESALEKGARFVLMFPAKALIKLARPQ"
     gene            complement(8620..9195)
                     /locus_tag="PKI07_01690"
     CDS             complement(8620..9195)
                     /locus_tag="PKI07_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOC49792.1"
                     /translation="MSTENNIVLGVTGSIAAAKAVELASLLTQKGCAVRVVMTADAQR
                     FVTAVTFKTLSRHPVITDLYDAEDGWRPAHIKLADEADLLLIAPATARTIARLALGLA
                     DDALSCVALALNPNAKTLIAPAMNGKMWLHPATQQNVATLKARGVEFIGPEKGVLACG
                     DEGVGRLWPVAQVAERALALLRRRPLARRRD"
     gene            complement(9201..9896)
                     /gene="gmk"
                     /locus_tag="PKI07_01695"
     CDS             complement(9201..9896)
                     /gene="gmk"
                     /locus_tag="PKI07_01695"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOC49793.1"
                     /translation="MMNGENHEPRPGAPGPESGAGSAAGLSPASSPLLVVISAPSGAG
                     KTTLCQQLLAARPRMTRVVTCTTRPPREGEREGVDYYFLDAAAFLKRVQAGHFLEHAT
                     VYGYSYGTLKSEVRGKLRQGQDVLLNVDVQGAATIRERAREDPELSRALVSVFLTPPS
                     MASLEGRLRKRGTDAEAVIQKRLGVARQEIAQWRHFDYLLISATIPEDLRRMLTILEA
                     EKLRSTRAPAPEL"
     gene            complement(9893..10774)
                     /locus_tag="PKI07_01700"
     CDS             complement(9893..10774)
                     /locus_tag="PKI07_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOC49794.1"
                     /translation="MKSMTGYGRGDCSKDGFTVTVEISSVNRKQSEIAISLPRQLELL
                     EAQIREVISGCVARGRLTARVSLHAGAGPAAPRVRLNVPLARAYARELTRLARQLGLA
                     GPVTLDQVTRAPGVFQTDEDLAVEEDFWPAVQKALKRALAQMVKMREREGAHLARDLA
                     KRVAVMRKAAARIAARAPMVARHYRRQLLERIKSAGLEAPVANDERLLKEVVYFADRA
                     DISEELTRLQSHFMQFEDGRKAKEPVGRMLDFLAQEINREINTIGAKANDRLISREVV
                     TLKAELEKFREQAQNVE"
     gene            11959..13116
                     /gene="nifS"
                     /locus_tag="PKI07_01705"
     CDS             11959..13116
                     /gene="nifS"
                     /locus_tag="PKI07_01705"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOC49795.1"
                     /translation="MNSPARCYFDHNATTAVAPEVVEAMLPFLREYWGNPSSSYTFGR
                     QLAGPLGEARARVAALINADPREIIFTSGGTESNNAAIHSALATQPGKRHIVTTAVEH
                     SATIKFVQLLQKQGYAVTFLPVSPDGALDLDQFEQALRPDTAIASVMWANNETGLLFP
                     VQEIAARCRRRGVLFHTDAVQVAGKLPIDAQALEADFLSLSAHKLHAPKGIGLLYLKR
                     GVKYQPYIVGGGQERGRRGGTENVANIVAFGRAAELARANLAQAETRVRALRDRLEAG
                     LLRQIPETVRNGAREPRLPNTSNLAFAGVEAEGILLLLDQAGICASSGSACTTGSLEP
                     SHVLTAMGCSAARARGSIRFSLGIANTAAEVDYALERLPGIIARLRALTPA"
     gene            13182..14129
                     /locus_tag="PKI07_01710"
     CDS             13182..14129
                     /locus_tag="PKI07_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49796.1"
                     /translation="MRPPSLMTAVRLLALAALLAPLAGCVSTPPPEQRARLERARALH
                     GTRATYCRPPRKADNRVDVDRLVAELVELRANTYSFCIHNYPTDWEDLKLLLPRAREK
                     GIRLWASLVPPSESPPHTRHYSEPFRLDYQQWAVEFAKLSLRETNLVAWSIDDFTHNL
                     KTPYTPEYVRQMLEAARAVNPRLAFAPCCYFTGITRPGFVTNYCHLLDGILFPYRHES
                     AGPNLKDPGLVESELQQLKALVGPDFPIVLDIYASAHSRLGATTPEYVEQAMLAGRRA
                     ADGVMIYCHQDPAKNPEKYQIIKRLFTRWAAEKERRPRR"
     gene            14341..15543
                     /gene="trpB"
                     /locus_tag="PKI07_01715"
     CDS             14341..15543
                     /gene="trpB"
                     /locus_tag="PKI07_01715"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOC49797.1"
                     /translation="MTAIATDAVPDARGHFGPYGGRYVPETLMHPLQELEAEYARARR
                     DPGFQREFEYYLREFCGRPTPLYFAERLTRELGGAQIYLKREDLLHTGAHKINNCIGQ
                     ILVARRMGKTRIIAETGAGQHGVATATVAAMFGLKCVIYMGAVDCERQALNVLRMKML
                     GAEVVPVKAGQQTLKEAINEAMRDWVTHIRTTHYILGTVYGAHPYPLMVRNFQRIIGD
                     EARRQILEKAHRLPDWLVACVGGGSNAMGLFYPFLNDESVKMVGVEAGGEGIATGKHA
                     ARFQGGSLGVLQGACSYLLQDEQGQIQLTHSVSAGLDYAAVGPEHAWLRDCRRVEYTA
                     ATDAQALNAFTELARLEGIIPALESAHAVAEVIRRAPTLSREQIIVVNLSGRGDKDVA
                     QAAQFISF"
     gene            15673..16020
                     /locus_tag="PKI07_01720"
     CDS             15673..16020
                     /locus_tag="PKI07_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOC49798.1"
                     /translation="MSEQPLLDAKNERIISLTKETQFAANGIVSRTLLRTDVAQVTLF
                     GFAEGQELTEHTSTQHALVQILSGECEFSLQGKPNILKPGDFFYMPPNLPHAVKATKP
                     FSMLLTLFKPSKA"
     gene            complement(16156..17874)
                     /locus_tag="PKI07_01725"
     CDS             complement(16156..17874)
                     /locus_tag="PKI07_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOC49799.1"
                     /translation="MKPSPSQPLAGILEPVFAIRTEEDLGIGDTEGVRQMIDWCHRHR
                     LNILQILPINELGNDNSPYNALSSLALEPSTLALSPRHLPDLTPETFLRLAPPDRVAG
                     LRRGPVNYPEVRALKHRLLEAAFAHFAAQHLDPPDERGRQFHAFAAENRAWLDDYALF
                     RALVEENGNTPAWDRWPPEHHSPAAARDWLRSLPRERRQELARRQLFFQYVQWLAFTQ
                     WQALKACGAARRVYLMGDIPFGVGRYSADVWANRDLFDLDWSGGAPPETTFKADPFTV
                     KWGQNWGIPNYRWEELRRRQFGWWRTRVGNLQKLFHLYRIDHVLGFFRIYSFPWTPDR
                     NAEFLPLTEAEAAARTGGRLPGFKPFPDDTPEHKAFNQAQGEEILRVVLAGSGETTVV
                     AEDLGVVPDYVPPTLLKLGIPGFRIPMFFREPDGAYADPKRYPRLSLVQPATHDHPPL
                     AAAWDGLWQAINAGTEADASRRELRQIMAFAGLRDIEPPREFAGQLHEAYSRAVLQSA
                     SWLAVFQITDLFAMTARFNTPGTVAQTNWTYRLPHTVRELEEDPVRRQQAETFSRLAR
                     ESGRGC"
     gene            complement(17871..19067)
                     /locus_tag="PKI07_01730"
     CDS             complement(17871..19067)
                     /locus_tag="PKI07_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49800.1"
                     /translation="MSTFDRRYEQISRSELEQMQLERLQALLARLRRNVRRYREKLGD
                     FRLQSLADLPRLPFTTQEDMVESFPYGMFAFPLREVIRLHSTLGPGGKPLVIGHTRND
                     LALWSRLVARQLSASGVTANDVILISLGGLTQSGAVGYVRGAELIEASVIAEDPLHID
                     YQLAMLQNYRPTTLITTPTNALDLIRAIEQRRVDPQSLYLRTVLLSRPVDRETREQIA
                     SGLFAAVRCNFGLGEILDPGLCVECEVGLFHIHEDQFLAEVCEGELVITTLAREAMPL
                     LRYRTRVAAELCHDQCACGRTGVLFQPGPRLDHRHQVREISFYETQVAEVLAQTRAAG
                     LRFHLTPSERQLQIALEISEDYFASAMWPADDPKRSIEAELLARLGLPAEVRYVEPRA
                     GSGPAA"
     gene            complement(19094..19795)
                     /locus_tag="PKI07_01735"
     CDS             complement(19094..19795)
                     /locus_tag="PKI07_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC49801.1"
                     /translation="MLLQVRNLSCGYGSLEVVHGASLHVCHGEIVGLLGANGAGKSSL
                     LKAIIGLLPPWAGEVFIDRRPTTGQPPWRSVSNSTVLVPEGKMIFADMTVRENLIIGG
                     YHNPDRIRHLETVLDGFPRLRERLQQVAGTLSGGEQQMLALGRALMARPRLLLLDEPS
                     MGLAPLMVKEVFTSIRKMKDLGLTVLLIEQNANAALRIADRAYVMETGEIVLEGPAAD
                     LAHNPEVKRAYLGKG"
     gene            complement(19780..20613)
                     /locus_tag="PKI07_01740"
     CDS             complement(19780..20613)
                     /locus_tag="PKI07_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC49802.1"
                     /translation="MALLEVTDVRRAFGGLAAVDGVSFTVKPGQIKGIIGPNGAGKTT
                     LFNLISGLLKPDAGRITFRGRSITGLKPYEIAGAGLSRTFQNPSLFLQMNVLENVMVG
                     RHCRTRWGFLGCGLRWPGQAREERAIREAARAQLDVVGLAHLAGWSAGALPFGQRRMV
                     ELARALATEPAMLLLDEPASGLNTREKEDLGRLIRQIRDRGITVLLVEHDMSVVMGLS
                     DDLLVLYNGRPIAEGAPAAVQNDPRVIRVYLGEDLQNDESGGDAPPRAPATPPATCCS
                     R"
     gene            complement(20600..21691)
                     /locus_tag="PKI07_01745"
     CDS             complement(20600..21691)
                     /locus_tag="PKI07_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOC49803.1"
                     /translation="MPRLRLLFLLALFPVMVAGAQLGLRAAGMEYCLTQLTMSVYYAL
                     VVMGLCLLMGYAGQVSLGHGAFFALGGYTTAVLTTHDFSGIRACAGVGVLERAGLLVA
                     QQDLYGQAGLSVAPWAAFAAAMLLTFVVALLIGYPALRLRGHYLAMATLGFTLIVSKL
                     LLGSALTGSADGIHGVPEWKLLPGLTVSGRSALRVQNYYLACGLALLLLLGLRNLLRS
                     RVGRALLAIHDRETAANAMGINTAAYKLKVFVLSALLAALAGVFFTHYTGGIAPSEAG
                     ALKSVRYVALAAAGGMANLWGVTVMSIALNYCSLRGWFGSYDNAVFGALLILIISLAP
                     EGPLKPLGLWLRRLAGRPDPERRTPHGAP"
     gene            complement(21792..22685)
                     /locus_tag="PKI07_01750"
     CDS             complement(21792..22685)
                     /locus_tag="PKI07_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOC49804.1"
                     /translation="MQNESLALQYLLSGITKGSIYAVVAIGFNLIYSATGIINFAQGE
                     FLMLGGMVAVTLAAYVPLPVAVTGAVALVALVGGLLELVFFRRLRRHSILHLVIITMG
                     LSIVIQETALHIWDEKVRSLPYFTGNEVSSVRFLGAAISTQVLWVLGTVALAVLGLHF
                     FLRYTLTGRAMRACSSNPEAAMLAGINIPNMRTLAFGLSAALGALAGCVVSPMTMTHY
                     EMGAPLAIKGFAAAILGGLGNPMAAVAGGLLVGLLEAASVSCLPAVYNDVSAFVILLL
                     VIFVRPQGLWGALAPDRVREH"
     gene            complement(22767..23930)
                     /locus_tag="PKI07_01755"
     CDS             complement(22767..23930)
                     /locus_tag="PKI07_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC49805.1"
                     /translation="MKRIFVVMAGIGVLGTLWLTSSCSPPPPSEPVKIGALFSVTGPA
                     SFLGAPEEKTVQMRIEQINAAGGINGRKLQLVVKDTAGSPEKAVSFAKQLIEEEKVLA
                     ILGPSTSGETMAIKDLCEQNQMLLVSCAAAETIVNPVARYVFKVPQKDSQAATWIYRT
                     MKEKGISNIAIVSSTDGFGAAGKKQLEDLAPAEGITIVANEVYDKQATDLTDVLTKIK
                     GNPKVQAVVNWSIVPAQSIVAKNMKQLGMDMPLFQSHGFGNRKYVQQAGAAAEGILFP
                     ASRLLVVEDLPANHPQKEVLATYKKDYESRFKEDVSTFGGHAYDALLVVVEALKKAGE
                     PEREKVRDALETLTGVVGTAGIFNFSPTDHTGLDLTAFEMLTVKDGQFVAYKQ"
     gene            complement(24263..24652)
                     /locus_tag="PKI07_01760"
     CDS             complement(24263..24652)
                     /locus_tag="PKI07_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49806.1"
                     /translation="MNVLDENILESQRLSLLGWRIPVRQIGHELGWKGMCDGEIIPFL
                     LTLRRPTFFTRDLDFWSRQLCHARYSLVILAVGQYEAAHFIRRMLRQPGFNSQSKRMG
                     QVIRVMHRGFVVWQRHAKHEVRHDWRD"
     gene            complement(24649..24873)
                     /locus_tag="PKI07_01765"
     CDS             complement(24649..24873)
                     /locus_tag="PKI07_01765"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOC49807.1"
                     /translation="MSDPKVCHGEPTFRGTRILVADVLEQVAGGIAWESIVEEWRGSI
                     TREAIAEAVRLAGETFREHAHELGLEPARV"
     gene            complement(25202..27292)
                     /locus_tag="PKI07_01770"
     CDS             complement(25202..27292)
                     /locus_tag="PKI07_01770"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC49808.1"
                     /translation="MKRTLRSVIGLTVLLGAGLLLEPASAQIQISSLPGGVYSQNFNS
                     LAISGANNPWQDNATLPGWYAGKSNKGTPDSFAKYTAGTGSDNAGALYSFGATGSPER
                     ALGSLASGNDKVGNIAYGVRFANDTGTDIASITITYTGEQWRNGGNTTAQTLACSCRV
                     STDPITSPDPTNADTWTPLTALDFISPTVGGGASAVSGNDAAHRQEFTDILLTGVVLP
                     AGAELFLRWRDVDDTGTDHAMAIDDLSVTFSSGTISTSAPTYATVALSAPTNQAGATV
                     LLIATSDGSPSQSLTYQWRKDGAALSDAGNLSGSTTPTLTLTNVLAANAGDYDVIVAN
                     SAGAATSAVATVTVLDPAIQSQSADRTCPEGTNVTFTVTARGTPTLIYQWFFGGAPIA
                     GATRSALTLTDLQITHQGAYTCWVTNGLGDYVASAPIQLTVTRFPSVTLALWDFNDTN
                     APVASPTPSLGAGTAALLGGVKATYAAGVSSDPRPTGTNQAWNTSSYPSQGSGNKTAG
                     VQFNVSTLGYQNIYLSWSQRHSNTGSKYTRLQYSADGETFSDLDFNTMTNGETFVSLA
                     CDLATWPAAHNNPNFAFRIVTEFESPANPNYVGTESTYSPAGTIRFDMVRVYGDPIGP
                     ETVAPVTISRLQGTTLSYGGGGGAQFVLLEASDVAAPLGSWARVQTNFATPGTFTIPA
                     VGAAPAKYYRIQSE"
     gene            27580..28860
                     /locus_tag="PKI07_01775"
     CDS             27580..28860
                     /locus_tag="PKI07_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49809.1"
                     /translation="MLTTVELMPEAPPADRVGKVLPRWLREVPLYQRAAKLLPLNGKP
                     LALEDLRRLPLITKHDIRRDFPRNFLRAGVELDTLLDQDLVELEQTSGTSEERVPLLL
                     GRGWWAEQEERALRLNPLAARVLDDFPAARRVTITSPVCSGDICYSGVPTHADRIVGN
                     ALFVSLSRYPFLWSDAELARMAGEAADWQPQFLDVDPVYGVLFALYCERQRIRLPSLR
                     FILSSYEFVSAAHRRILQRVFGVPVLNLYGSTETGHLLMETGPDEMRPSQETALLEMV
                     AADEQGIGDLVVTTLTNDFMPLIRYRIGDLVEQLGPPRAPRYVVHGRAADAFFTPAGR
                     RVTTWQIDQCLADLPGMVHYQLCEQPDGGWLLRFVPDPAAPDAAPPAELQRRLVERLE
                     IRGQLVVQATDLLVPERSGKFRLGYPARKPTPAP"
     gene            complement(28899..29696)
                     /locus_tag="PKI07_01780"
     CDS             complement(28899..29696)
                     /locus_tag="PKI07_01780"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC49810.1"
                     /translation="MPIKLISTDFDGTLFAEFESPPIPASVQQRIGELQERGARWVIN
                     TGRDLSSLMEALARARIGIEPDYLVLVEREIYQRADSQYLGLAEWNAACARDHAELFA
                     RVRQDLPRLIGWISARFPARLYEDTYSPFCLIAGNNGDADAIHAYLAEYCRGIPNLGL
                     MRNDVYARFHHVGYDKGTALAELTRRLQLGPAEVFAAGDQLNDLPMLSRQYAHWLAVP
                     GNAVDPVKDAVRRQKGFVSQLAHGNGVAEGLDFCLRQAQRLRPARTP"
     gene            29798..30079
                     /locus_tag="PKI07_01785"
     CDS             29798..30079
                     /locus_tag="PKI07_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49811.1"
                     /translation="MKTAYELAMERLGKTAPPVKLTAAQKREIAELEAQCTAKIAERE
                     LLLKGEIARAIDKGDAEALEQLEKQLVSDRKTLRAECEAKKEQVRKAAG"
     gene            complement(30129..30710)
                     /locus_tag="PKI07_01790"
     CDS             complement(30129..30710)
                     /locus_tag="PKI07_01790"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOC49812.1"
                     /translation="MALKPWKKLGSQPAGEFRIFSIRSDRVVSPRTRQAQDVYVIDCV
                     NWVNVIALTPDQHLVMIEQYRHGSTTVELEIPGGMIDPQDASPAAAALRELREETGYE
                     GEHPRLLGRVFPNPAIMSNTCFTVLVENCRRRHRVQLDQGEDLITRRVPIADLPRLVA
                     AGRIRHSLVVAALHYFDLWRRGIAPEDGPLRGA"
     gene            complement(30711..31448)
                     /locus_tag="PKI07_01795"
     CDS             complement(30711..31448)
                     /locus_tag="PKI07_01795"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOC49813.1"
                     /translation="MIAEDNFVDFTCPYCGDPVSFPRESAGFVQACPNCTESFIVPDD
                     GGAVGRAIPLPITTARLVIRRLAAQDWQDLLELMADEEFFRYTDGVPLDEDGILRWLA
                     SDAHVKLTTPDQPFFLGLELQDGGKLIGYATLNFTDPQWRQVNFSLGLNRKFQRQGYA
                     RETVAALLDFCFRGLKLHRVASWCDHRNAAGLRLWEKAGLRREGEFVKNRWVHGEWAT
                     SVWFAALAEDYFPSQGQALPAGGSEPG"
     gene            complement(31520..31813)
                     /locus_tag="PKI07_01800"
     CDS             complement(31520..31813)
                     /locus_tag="PKI07_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49814.1"
                     /translation="MRAKLVAVICVAAITLGGAASCPAADDHGPLAVVADAAVVRPAC
                     LVATAIGSAVFVVALPWAAASRSIKETAQTLVIKPAQATFTRPLGDMDALLEE"
     gene            31941..32016
                     /locus_tag="PKI07_01805"
     tRNA            31941..32016
                     /locus_tag="PKI07_01805"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31974..31976,aa:Ile,seq:cat)
     gene            complement(32520..32663)
                     /locus_tag="PKI07_01810"
     CDS             complement(32520..32663)
                     /locus_tag="PKI07_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49815.1"
                     /translation="MSKARVTVAPSEPPPRHANIEGWPDEKSARKLIAIDLANAAQLV
                     VKP"
     gene            complement(32700..32918)
                     /locus_tag="PKI07_01815"
     CDS             complement(32700..32918)
                     /locus_tag="PKI07_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49816.1"
                     /translation="MSGEGINGSGIAPEDPLARFIYSASHFSRGNQRVKHNAFMPGAD
                     GKTSVFQTKGLDEPATWGIGEAVGAKRS"
     gene            complement(32915..33115)
                     /locus_tag="PKI07_01820"
     CDS             complement(32915..33115)
                     /locus_tag="PKI07_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49817.1"
                     /translation="MAVDPDGEVSLEWYCPTGRVFSVSIGANDGLSYAGKFNPSRKTH
                     DTEIFTNQIPQVILDNIRRVIA"
     gene            complement(33287..33484)
                     /locus_tag="PKI07_01825"
     CDS             complement(33287..33484)
                     /locus_tag="PKI07_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HOC49818.1"
                     /translation="MKVTYDPEVDVLRILFRDTAIEESDEDKPGVILDYDKDGNVVGL
                     EVLNASKRVENPRGVEYAVTS"
     gene            complement(33481..33741)
                     /locus_tag="PKI07_01830"
     CDS             complement(33481..33741)
                     /locus_tag="PKI07_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOC49819.1"
                     /translation="MRHELSSHVQQEMERRGIPLAVVESVLDAPGQKVPEHGDVVCYQ
                     SKVEINQKPYLVRVMVNQTVTPMKVVTVYRTSKISKYWKTTT"
     gene            complement(33738..37361)
                     /locus_tag="PKI07_01835"
     CDS             complement(33738..37361)
                     /locus_tag="PKI07_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOC49820.1"
                     /translation="MPAELTASSRKQQQVLDRLESPIKTRDDLLRLFVNTLGYQRVER
                     PIPISKDTFGEGIALDLAQQYRPLRLAEAGAFHVIYTELDGDRLDYRRQRVLASKLLE
                     TFPDALFVFARAGTLGDSRGAEAHLVNVKTADRRVFRRFKLGPDERYRTAAERMALLD
                     VSEVTDLSMLAIRARHEAAFDVEPVTRQFFRDFCSVFDLVCKDIARRQSRWNSEHVER
                     EAQTLLDRLLFLYFIQRKGWLNRDRNFLYRHFRQHLAESKDSATYLEKFLKPLFTKLS
                     TEGSMADIAGFDLPFLNGGLFADEYGAEQRDKSARRRHSLKVCNSVFAHVFDELLEAY
                     NFTVREDTPLNQDVAIDPEMLGKIFESLVLQIEQTDTSGKTSRHDTGSHYTPRPIVHY
                     LCRDGLRIWLEQAPPDPARAQDWPDRLQKLLALDASDGVDPETMAVLESCLTPDEARV
                     LLERLDNLRACDPAVGSGAFPVGLLYELVNLARLCETRLRGKDPVADDPEWLFITKSR
                     FIQRVIYGVDIQERAVEICKLRLWLSLIVDHPLDVDVDKCSAKDFRHALKKLPALPNL
                     DFKIRVANSLIDRVHGEPVNLTSVAAEDKTLPPILNKLTGAKRQFYEAHSITDKRRLR
                     FDILDATAELAMVELARSKLSFGLLVDESDEAAMTRLAELDRALKEMGAVREQIAAAR
                     RPKTKASVQDETLERLTERFNDPKKPTFVWQLDFAEVFHRFIGARNGDLAPEESKAAA
                     DSSAKSGTGRTGGFDLVVGNPPYIRIQVLKGIAPEDAAYFEDHYASAGKGNYDIYVVF
                     VERSLQLMNPKGVLAFILPHKFFNAQYGEPLRAQLAKGRHLSHVVHFGDQQIFPGATN
                     YVCLLFLSRAGADACRWVRADNLPDWLNTLRAPETSLPAARFTPAEWNFAVGTNAGLQ
                     DKLQALPIKVARIATRIAQGIRTSANEVYVLDVRSEKGGLITAYSKQLDQDVVVERKA
                     VLRFLQGREIKAYSIQSSNKVVLMPYRIQGGDATLASERELAQAMPRAFDYYRANKHY
                     LSQREGGRFKGPEWFQFGRKQNIDLMLLPKILVPDIADRAAFALDEKGDFAFTSGYGI
                     TFREGVRESTKYFLALLNSAVLDWYWRRISTPLRGGFFRYFTQFIEQLPIVPVDFSNA
                     SERAEHDALVSLVERILSAKRAAPDADTTALEREIDERVYRLYGLTADEIKLVEESTQ
                     SKP"
     gene            complement(37364..40633)
                     /locus_tag="PKI07_01840"
     CDS             complement(37364..40633)
                     /locus_tag="PKI07_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOC49821.1"
                     /translation="MPHDIIDNRELLLADAVRPLLCESVRAHFAVGYFFLSGFKAIAD
                     QLAHVRELRLLIGNTSDRATIEQLAEGHASRAAIIAHQREGAFLNARQRAELVAEGAR
                     RIRERLERLDQTDDDQALITQLVRLISAGRLKVRVYTRARLHAKAYIFDYPTHRFERG
                     IAVVGSSNLSLAGLRDNTELNVVVHGNGNHEQLLTWFNRLWDEAEPFDPELMTQLQSS
                     WALDESVTPYELYLKVLYHLTKDRLEEPDVPMPADFPPLADFQWAAVKSGIRLLRLRH
                     GVFIADVVGLGKSFVAAALLKWLKVRERQRALIICPTPLVKMWRDRFVDRYDLGAEVL
                     SLGMLSQPNFAFEDEKYQNKQLVLLDESHNFRHSDSLRYDRLFQFVRSLALPVVLLTA
                     TPRNSRARDILNQIRLFNADDRIDLGVEPRSLGKYFKLVENDERPLPPLLQHFLIRRT
                     RKHIREHWPDAKVDGRSVRFPKRNLKTIEYSIEKTYGGFYKTLRNLIEPPNPGKKKKV
                     GMTYARYGLHRYVVPAKQDVAPYHDLVRAGQRLHGLMRTLLFKRLESSVEAFRQTVTR
                     LIERHKIFLNGLDDGAIIAGEGVEDLLKGVEEGDSENADLRAELEKLSQKYDPADFRL
                     DDLRRDVAADMATLKEMAKHVEPITPDKDAKLQRLLQWLDDEPLLRTHKLLIFTQYID
                     TAQYLTEQLRAAKIRPPETIEHADSSRDDLQTLVSRFAPIANEVKAPVQNPIHVLVGT
                     DVLSEGLNMQDAALILNYDLHFNPVRLIQRFGRIDRINSPHTEIFAFNFLPELELERH
                     LGIRTILRERIEDIHQTIGEDAAILEPDEQLNADAMYAIYEGDGKRLEAIEDDMDRQV
                     DLEVQEAEDLLRRIKRQQPELFARITSLPNAIRTAKRSTADPTTPPRPVIFFFGQAGD
                     FQRLWLADAEGNIVTEDNHSSLAAITCPPTEKRQPLPPQYNALVTALKAKFDQQYQDY
                     LAAGGTPHRLTASQRWALDTIRDAYKQAAELLDQTPETKDQCARLERLRSLWSISPLD
                     GRVELALRGLRNQKPARDEALARLESLAFEHKLDVLAERHAEVAKLATEPAIICTEAL
                     V"
     gene            complement(40913..42052)
                     /gene="gcvT"
                     /locus_tag="PKI07_01845"
     CDS             complement(40913..42052)
                     /gene="gcvT"
                     /locus_tag="PKI07_01845"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOC49822.1"
                     /translation="MKRTPLYAAHQRLGGKLIEFGGWEMPVQYTGIVEEHRAVRQGGG
                     LFDISHMGEIRVRGPGAEAFLNWTLTNDLRKLAAGQGQYTLLCNERGGVIDDLYAYHL
                     APWEYLLIINASRIEADVAWLEAQQARFPRRDDLTLENASDATGALAVQGPRVVEFLE
                     HCFPGPSSGGASVARVTALKKNQVAGFNHAGARIWISRTGYTGEDGFEVIAPGTLIEE
                     IWNQLLAAGFKHGLKPAGLGARDTLRTEMCYPLYGHELDENTTPLEAGLGFFVALDKE
                     DFIGRTALAGQKANGPARKLVAFKMTGKSAPPRPHYALWTTGPGAAKVGTVTSGTQSP
                     SLGIGIGMGYVPPACAAPDTPLEIEIRGQRAPAVVVPKPIYRKTP"
     gene            42360..43013
                     /locus_tag="PKI07_01850"
     CDS             42360..43013
                     /locus_tag="PKI07_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49823.1"
                     /translation="MNRTFVIILVLLVLALGGGLAWQYSRTTALKAELAGLRQAQQAA
                     SESSEALRTAQAELESLRLQAEELPRLRRDSQELIRLRNEVRQLRDSKGEFQQNLDAA
                     GEENEQLRAIAQQLRQLAAENEQLRTQLYQAENPSAPPATTSTKNLCARNLRQLDAAI
                     QQWALENQKTATDTVTSEDLMPYLPAGMPACPAGGQYTLSTVRAGPKCSIDGHALVR"
     gene            complement(43271..44281)
                     /locus_tag="PKI07_01855"
     CDS             complement(43271..44281)
                     /locus_tag="PKI07_01855"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOC49824.1"
                     /translation="MNTKQFLTLSSVSLVGASAALATADYGPAVDRMITGCSKWYTSG
                     YGHKFAVVHSMEGYYLTGTSYLRRCDISASCHYTVNGKTDYAGDAAPGEISQLVREAY
                     YAWHATCWNQHSLGTEHEGFVGNPAWFTDAMYQASALLHRHFADKFGFAKDRNHIVGH
                     GEKSNAAWCSYASANLGINPYCNTHTDPGPYWNWTYFMSLINPPAPVVVDNSNAGFTA
                     SSAWATGTAAGDKYGANYHWRSTQAVSDPASWSASNLPAGTYKIYAWWSAGSNRSTAA
                     PYIVYHSGGSTTVTMNQQANGGKWNLLGTFNLNAGTGKVSLSCWAPSGYVVVADAIKW
                     VR"
     gene            complement(44511..45194)
                     /gene="tsaB"
                     /locus_tag="PKI07_01860"
     CDS             complement(44511..45194)
                     /gene="tsaB"
                     /locus_tag="PKI07_01860"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOC49825.1"
                     /translation="MKILALEFSSAQRSVAVLAEGAGAGAAVLGEASETGTRATRAFG
                     LIEEALRQARLEREQIECLAVGLGPGSYVGIRLAISLAQGWQLARPVQLLGASSAEGL
                     AAQAQADGLRGRIGVVIDAQRGEFYLADYELTATARREVGPLRLVPGAEVLARQQAGV
                     TLLGPEVALWFPGSRMLFPRAAAIGQLAQGRRDFIAGEKLEPIYLREARFVKAPPPRV
                     GIPRREGPP"
     gene            complement(45175..45630)
                     /gene="tsaE"
                     /locus_tag="PKI07_01865"
     CDS             complement(45175..45630)
                     /gene="tsaE"
                     /locus_tag="PKI07_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOC49826.1"
                     /translation="MATLISHSPAETLALGEQWGRAVPSGWVIGLSGDLGAGKTQFVK
                     GLARGLGIAARVHSPTFALVNIYSGGRLTLFHVDLYRLETPAQILAAGLEDYLTPAGV
                     TVIEWAERWFGEAQDQPAAPFLPADRFRWIQIHTLGETARRITYEDIGS"
     gene            complement(45608..46540)
                     /locus_tag="PKI07_01870"
     CDS             complement(45608..46540)
                     /locus_tag="PKI07_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOC49827.1"
                     /translation="MNEFDLIRRLTRALPTNPGVVVGAGDDCAVLEPGGPDRLLLFKT
                     DAVVEGVHFLADAPPEKIGHKALARCLSDIAAMAGTPSAALVTVGLPRGFQADWVEAV
                     YTGLNALARRCEVAIVGGETTTSPGGVFISVALLGWVPRGQGVLRSGAEAGDAIFVTG
                     ELGGSLAGKHLDFEPRLAEARWLARHFPPHAMLDISDGLASDLRHLLSASRTGAELLG
                     AAIPISRAARLAARAGSGAKPALLAALTDGEDFELLFTVASRDAVPLLDAWRKQFPGL
                     PLTCIGKITAGEGIALRDKHGTRTLTDHGYAHFA"
     gene            complement(46537..47454)
                     /locus_tag="PKI07_01875"
     CDS             complement(46537..47454)
                     /locus_tag="PKI07_01875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOC49828.1"
                     /translation="MLTVKPWPVEAIVRLVLSVILCAYAGSVLISVQAYAAAGARGNA
                     KLFFPLAAGALVCLAAALGLIARAWPPEGISRRLAGLVVCAYSGLLLGAWAQQLSGAG
                     AGDASLSRMLMAALSFHGAGLILIGRFLREQQTHWTAAFGLAHRWRRAALMGALGALL
                     FLPMGWGLQKVSALVLTHLPHLGLTPKEQLPVTALREALTWGGRLSLGAAAVLLAPVA
                     EETMFRGILYPVIKQAGYPRLAWWGTALLFALVHLNLVTFVPLLVLALLLTELYERTG
                     NLLAPITAHLLFNALNFAALLVLQHYGGA"
     gene            complement(47599..>48167)
                     /locus_tag="PKI07_01880"
     CDS             complement(47599..>48167)
                     /locus_tag="PKI07_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49829.1"
                     /translation="PKSAKRCLLFQLNRSGLDSQLTVFQQITTRPPIPAHRFGLSRHP
                     HPLSRYLENTLPSRRVQGVYKGCPPGVHGILAGTPDGHPLYTPCTRPEGRVRSGLVYG
                     APKASGFRFCLPFTQLRVRKLLILQAKVVWGSVAMPLISLNKGFAGAIGLGGQGCLGS
                     EGTRVDDWFFQLNRFRMGDEFKVLPLAE"
BASE COUNT         8709 a        15693 c        15368 g         8397 t
ORIGIN      
        1 cttgtaaaac cagggactta cacggtttga agggtcgaat tggggttaaa atctgaatcg
       61 ttccggctaa aacgaatccg ggatcgagcg tattgaacgc aattattgac ccggccagac
      121 ccaagtatcg ctgactccgg ttcgcgaggg ggagccgcat cgcagggatg gctgaacccg
      181 gtaatgcgat atgcccgggt ccgggaatcc tccgaaaacg cccgggctaa caccgaatcc
      241 cgttgacggt gcggcgaggt tcctctcatt atgcgggcaa gttatttatg aatctcgata
      301 cattgtattc ggaattgacg cttaagacca acgccaagct ggtgctgctg gtgctcgatg
      361 gcgtgggcga tgtcgccacg cgggagcaag gttatttgac cccgctcgaa gcggcggcca
      421 cgccgaacct gaatgcgctg gcgaaagatt cggcgcaggg acggatgatt ccggtcgccc
      481 cgggcatcac cccgggcagc ggcccggggc acctgggcct gttcgggtac gacccgctgg
      541 agttccaggt cgggcggggg gtcatcgagg ccctgggcct ggggcttgaa ctgcgcgcgg
      601 gggatgtggc cgcgcgagcc aacttctgct cgctcgacgc caaaggcatc gtcacggacc
      661 gccgcgcggg gcggattgat acggcggtgt gcgaggagct ggtcgcactg ctgtcgcaaa
      721 agattaagaa ggtgggcgac gcggaagtga tcatcaaggc cggcaagggg catcggtttg
      781 tggtggtgtt ccgcggcaag ggactggaag ggccgctgag cgacgctgat ccgcatcgcg
      841 agggattgcc cgtgccccag gcggcgccgg tgaatcccaa gtccgtgaag gcgaaaaagg
      901 ccgccaagct ggtggccgga ttctaccagg cggccctgcc cttgctggcg cagaagaagc
      961 cggccaatgg gttcctgatg cgcgggatcg cgcatcagcc ggagattccg ctgtttcaag
     1021 accggtacga gctgcgcccg gcctgcattg cggtttatcc catgtataag gggctggcgc
     1081 aattggtggg gatgaccaag ctggaagggc cgcagaccat cgcccagcag ttcgagcgtt
     1141 acctggccga gcgcggcaac tacgacttct tcttcattca cttcaaatac accgatatgt
     1201 atggcgaaga cggcaacttc gccgccaagc ggaaagcgat cgaggaagtt gacgcggccc
     1261 tgcccatcct gctgcgccag aagccggacg tgctggtcgt cacgggcgac cactccacgc
     1321 cctgcgtgat gaaaggccat tcctggcatc cgcagccggt gatgctgcat tcggccttct
     1381 ccggctcgga caagctggag cggtttaccg acacgggcgc caacgccgga tcgttgggcg
     1441 tgtttccggc gaaatacctg atacgcctga tgcaggccaa cgcccggatg ctggacaagt
     1501 tcggcgcgtg atggcccggc tggtattcga tatcgaaacc tccgcgctgc cgctggagca
     1561 attcgacgac gcgcagcagg aatacctgtt ccgcgactgc ctgaagatgg ccgacgaggc
     1621 ggcgcaggcg gcgcggcgcg cggaaatcca gcaacaattc aacctgtggc ccttcacggc
     1681 gcaggtggtc tgcgttgcca tgctcaacgc ggactccttg cgcggccagg tgctgttcac
     1741 cgcggaagac ttcgaggagg aggccgacga ggccggcccg gtggagttca tggcgtgcat
     1801 ggacgaggtc gagctgctga cggccttttg ggatgtggcg aagcactacg aggccatcgt
     1861 cacgttcaac gggcgcggct ttgacgtccc tttcctttac ctgcgctcgg ccctgctcaa
     1921 tgtccccatt acgcgcaaag actggcttgg ctaccgtttc cagaccgaac cgcactgcga
     1981 cctggcggaa cagttgacct tctacggcgt cagcgggcgc gagggcgccg cccggcgctt
     2041 taacctggac ttctactgca aggcgttcgg gatcgagtcg cccaaaagcc agggagtcac
     2101 cggcgcggat atgaatgcgt tgatcgccgc gggccgctac cgcgagattg cggaatactg
     2161 cctgcgggat gtgaaagcca ccgtgctcct gtaccacctc tggcaggagc ggctggcggg
     2221 catcaagtga aggccggagg gaagccgccg ggtcccgccc ggaaggagtg ggcatgtttc
     2281 gcggtcaggg aggaagcgcg gccgttcgcg cggcgggccg ccgggcgggg cggcccccgg
     2341 ataatcctga ccggcatcgg ccgccgcaat gcggaggagg tcatccgcgc ggccctggcc
     2401 cgggaacgcc ccgcccgggt gctgagctgc ggttttgccg gcgggctccg gccggagctg
     2461 gcggcgggaa cggtgctgtt cgccgccggg gcggaaaccg gcctggagcc ggccctgcgg
     2521 gcggcgggcg cgcagccggc gcggttctat tgtgcggacc aggtggcggc caccgccgcc
     2581 gcaaagcagg cgttgcgcgc ggccaccggg gcggacgcgg tggagatgga gtccggcgcg
     2641 attagcgccg tctgccgcga gcagaacctg ccctgcgcga ccgtgcgcgt gattctggac
     2701 accgccgggg aagacctgcc gctggatttc aaccggttga tgaatgcccg ccgccagttg
     2761 gactacggca agctggcgct ggcgctggcc ctgtccccgg ggaaaatcag ggaactgctg
     2821 cggctgcgcc ggcgggccga ggcggccgcc gaaaaactgg cgcgggtgct ggccgcgatt
     2881 acggcggtgt agctcccgcc tcccgggcgg cggcgggccg caagggcttc ggtttggcgt
     2941 gctcgctgta gctgatcagg gcggcgccaa tcacgatgaa caccaccccg gcccagcgga
     3001 gggaggaaac ccgttccccc agaaagagaa tcgccgcgag ggtggtcatc acaaaactca
     3061 gggcggtcag gggccagagg aagctgacct cgcttttcgc cagcagaaac agcaggcagg
     3121 caaagaacaa cgcctcgaaa aagacgccca ggagcacgtt gccattggtg atgccggcct
     3181 gggcgacccg gaggatctca gccgcgctga cgctcttcac ctcgccgatc tgcccgatgc
     3241 cttttttgag caacacgacg ccggccgcct cgaagaccag cccgataagg aggatgaaca
     3301 gcagtttagc catattacaa atcctgatag cgaatcaatt caatgccaag gcgcttaacc
     3361 tgttcccgca cgcgcgggct gaccagggca tcgaactctt gcttaaagtc gtcgagcgaa
     3421 gggtgggagt aaagctctga ctcgcccgcc gggagctgcg ggagcaggcg ctggagatag
     3481 gcttcatcca cgcgcgcatt ttgcagcagg ccaaagacgg cgcgcggatg cttgatgccg
     3541 gaccgggcca gcgccgggcg ggcgcgcgcg gccagcctgt gaaaaatcac cgcgtgcccc
     3601 aatcgcgaga aaaagcggcc ggaggccagg cgcaggttca ggcggaaggg atccgaagtc
     3661 aggcgcaggc gctgaatgcc gagcggcgcg gcgtcgtcca tgagcaggcg gaatatcgtc
     3721 gggtgcagat gcaggtgcag atggccgttg acgtggtcca gcggcaggcc cgtggcgcgg
     3781 aacttcgcaa actgcgcatg gatttcccgc cgcaactgcc cgtgcagccg gcgccggaag
     3841 aaatagcgaa accccgcccc cgccggattg cggctgaact cgccgcgcgc gtcaaccagg
     3901 ccgggaatct cggcccgcgg cagaaccgcg gaaccgcaga ccagggtcag gtgcagcccc
     3961 acccccagcc gcggatgcgt gcgggccagc gcgaccgcct ccgcgaacgc cgctccgcct
     4021 accatcaggc tggcggtcgt caggatgcct tcccggtggg cccggatcac cgcctggttg
     4081 atggcggagg agcgtccgaa atcgtcggca ttgacgatca ggcggcgcgg gggggagagc
     4141 ggctggaatc cgctagacgc catagtcagc cggcccggcg cccgcaacat gccggcccca
     4201 cgcgcgcagg atgagcagca gcttgtgcgc ccggttgagc cgtatcggcg cggggccaaa
     4261 aacccggaag cgccgccgct ccagcttgcg cagcagccgc ccatagacgc accccatcag
     4321 ttcggccgcc accatcgagc ggcggtccgc ggcggggagc gtctcccggg ccgcctggta
     4381 aaactggcgc gcgcgcccgg ccacgctggc ggccaggtcg ccaaaccgcg ttgaatattc
     4441 cagccgcagg atttcctccg gcgcaacctt gaaccgggcc agctccgcca gcggcaggta
     4501 gatccggccg cgctcggcat ccgtgcgcac atcgcgcagg atattggtca gttgcagcgc
     4561 cttgcccagg ttgaccgcgt aatcgcggca ccccgggttc cggtatccga acacttcgat
     4621 gctgagcagg ccgaccaccg acgcgacgcg atagcaatag aggtccagcc gctcataatc
     4681 ttcataacgc gtgcagtcga gatccatctc cacgccttgc agcagggcgt caaaatgctc
     4741 aaatgccagc ccatactggt ggatgaacgg ctgcagttcg cgattgacgg ccaggcgggg
     4801 cgtttccgtg ccgcaagccc gccggacatc cgcccgccag gccgccagtt gttcgcgccg
     4861 ctgcgccgtg gaaagggatt cctcgtcggc cacatcatcc acctcgcggc agaaggcgta
     4921 aagggcggac atggcattgc gtttggccgc cggcagaagc acgaacgcca gagccaggtt
     4981 ggacgcgctt ttccgcgtga tcttccggct ggcctgcatg gggcaagtct aaggggcggg
     5041 cgggatgtca ggacgaatgg cgggcaatcc gccggtttcg gccagggtcg gattgcggcg
     5101 gcggtgtttg cgccgcgagc gccggcgccg gcgcggcgtg cccggcgcgg ccgccgccgg
     5161 cccgttgacc gggccgcgcg ccgccggacg cggtcgggag gcatgatgat gccacgaatg
     5221 ccgcgaatgg cggcggcgtg gtttccgcag gtaaaccgcc cacagcacca gcaccagcgt
     5281 caccgcggca agcgcgctaa acatcagcag ggccgcaggg ctgggcggcc gtatggattg
     5341 ccacgttctg accacgtcgt ccacggtaac gacgttcagg aaaaacagcg gcaggctcat
     5401 ggctaggggg ctttgcccgg cgcggcggac gagtcttcct ccgcgtccgt cccggtcgtg
     5461 atgttcaacc gctgcatccg gtagcgcagg gcatggcggc tgacttttaa ttgttccgcc
     5521 gtccgggtca ggctgtaatg gttctgctcc agcatggccg tgatgagctc gcgctcgaaa
     5581 tccgccatcc ggtcgtccag cgactctgaa ccggtgaggc gcggcgcggc ctttttgtgc
     5641 aggcgcccct ccaactggaa gtccggcagg ctcgccgcct ggatttccgg ggtggtctcc
     5701 aggatcagcg cccgctcgat tacgttgcgc aactcgcgca cgttgccggg ccagtgatgc
     5761 gcctgcagtt tctgccgggc ggcgcgggaa atcgcccggg tctttttgtg cagcgcctcg
     5821 ttgaaggcct gcaggaagta atcggccagc agcagcacgt cctcgccgcg ctcgcgcagc
     5881 ggcggcaggc ggaactgcac cacgttgagc cggtgaaaca ggtcctcccg aaactggccc
     5941 tggcgaatgg cctcgacgat gtcgcggttg gtggcggcga tcagccggac gttgacccgc
     6001 aattccgcgg tgcctcccac gcgcaggaag gaaccatcct ccaggaaccg caggagcttg
     6061 gcctggaggg ggcggctcat atccgcaatc tcgtccagga aaatcgtccc gccgtcggct
     6121 tcctccacgc gcccgggctt ctgtttcaac gcgccggtga aggcgccctt ctcgtggcca
     6181 aacaactcgc tttccagcag ggcttccgga atggcggcgc agttgatccg cacgtacgcc
     6241 gccccggaac gctggctcag gctgtgcagg gcgccggcca ccagctcctt gcccgtgccg
     6301 ctctcgccca gcaccagcac gcgggcatcg gtcggggcga ccgtttgaat aagctggcgc
     6361 aaatcgcgca tcttctggca ggcgccaacg atctggtcga gccgccagct ctcggccgcg
     6421 cgcgcccgca gcgaggcgtt ttcccgcagc aggcggtagc gctcggcgca ccgcgccgcg
     6481 gcgtgcagca attcctcggg cgcgaacggt ttggccaggt aatccatcgc gccggccttg
     6541 atggcctcga cggccagctt cggcgtggcg taggccgtga tcatcaaaac cggcaggtcc
     6601 ggccattgcg cccgcacctt gcccagcaac tcgtagccgc tcatcccccc gagccgcaca
     6661 tccgtgatga ccatgaagaa ctcctcccgc tccagcagtt ccagcgcttc ctcggccgat
     6721 tccaccgtcc gcacgccata ccgctcgtcg gtcagcaccg ttttgagcga caggcgcatg
     6781 ttcttctcgt catccaccac gagcaccggg ggcaggcggc tgctcatgat cgcggcctcc
     6841 tttccgccgg cgcgggccgc ccggggcgcc caccccacac ccccgcgccc gcgcgggcgc
     6901 gcgttttcgc cgggagctgg tgagcaagcg gtttcactgc ggtctggcca gttttatcaa
     6961 agccttggcg ggaaacatta gcacaaaccg cgcgcccttt tcaagcgccg attccacccg
     7021 cacgctcccg ccgtacagct cgacattatg tttgaccgtg gccagcccca ggccggtgcc
     7081 tttctctttg gtcgtgaaat aggcctcgaa gattttctcc agcttgtcgg gcggaatccc
     7141 cggcccgtca tcgcgcaccg cgatttcaat cgaatgatcg ccatggcaca cggcgctgac
     7201 ggtgatctgg cccccggcgg cgcccagggc ttcgcgcgcg ttctgcagca ggttcaccac
     7261 cgcgtccacc aggtggcgcc gctgcatgaa cagcggcgga aactcgccgg catactggcg
     7321 gtgaatccgg accggatact ccgccgcggg tggaaaaacc tgggcaatgg ccttgtccag
     7381 ctcctccccc acggccagct tttccacgtg gccctcgctc aactgcgcat agcccatgat
     7441 ctgggtgatg atccggtcgg aatgcgccac ctcctcctgg atgatctgga tttgctccgc
     7501 cccgatcggc cgcccctgcg tcagcgcccg ctgcaggctg aaggccgcgt tgttgataat
     7561 ggcgagcgga ttcttcatct ggtgcgcaaa ctccgccgcc acgcgcccgg ccgagcgcaa
     7621 ctggccctcc cgcatggcaa actcgcgctc ctcttccacc acccgccgct gccgctccag
     7681 cagcacttgc accccgtagc agcagatggt catcagcacg agcagcgcca gccgcagcgt
     7741 cagcgtctgc gtcggctcat ccgccgaggc gtagaatccc aggctggccc gcatggattc
     7801 gtccaggttt tgcgcggcag ggtgccggct cggaagcggc ggcgcgacgg gcaggagctg
     7861 gtccgtcgtc tcgggcggcg cgaccccctc gggcgcgggc gggggagcca acccgcggac
     7921 cggcggcgcg ttggaagccc gcgccatgcg cagcggggcc ggccacgcca tccgcgtcag
     7981 atactcctcc tccagggttt tgttgatctc gatgttgacg gagccggcca tgatatagca
     8041 gccggtcagg gtcaggttga gcagcaactg ggaggtcgcc cgcggaacgc tcaccgcccc
     8101 gcggataatc agccccagga aaagccagta cagaatgctg tcgtatcccc cggtcaccac
     8161 caccagcgcc gacaggaaga tgccgtccac caggctgatg gcaaacaccg cccactggac
     8221 cagcaacagc ggcagccgcc gcatgcccag caacaggccg cccaccacgg cgttggccgc
     8281 aatatagacc cagagaaaat actgggtggt ctcgacggcg atctccagcg tccccagttc
     8341 tttgaccccc acccagcgct gcttgaaata gaacgagtac agcagcatgg caatcaccgc
     8401 cgccttgatg ggcagcacca ggtcccgctc cagaaacgcg atccggtcct gcagcttggc
     8461 cgcgtccggc gcggacagct tcagcagctc caccgcgcgg cccgggagcc gccgcagcat
     8521 cccggccagt tcccggaggc tgtaatgact cagccgttgc cacaggccca agttcatatt
     8581 ctgccaggct ccccggggcg ggcgggttcc tccgggaaat caatcgcgcc gccgcgccag
     8641 cgggcgccgc cgcagcagcg ccagcgcgcg ttccgccacc tgcgccaccg gccacagccg
     8701 ccccaccccc tcatcgccgc acgccagcac gcctttttcc gggccaataa actccacgcc
     8761 gcgggccttg agcgtggcca cgttctgctg cgtcgccggg tgcagccaca tcttcccgtt
     8821 catcgcgggg gcaatgagcg ttttggcgtt cgggttcaac gccagcgcca cgcagctcag
     8881 ggcatcatcg gccagcccca gcgccagccg ggcaatcgtg cgcgccgtgg ccggggcgat
     8941 cagcagcaaa tccgcctcgt ccgccagctt gatatgcgcc ggccgccagc cgtcctccgc
     9001 gtcatacaaa tcggtaatca ccgggtggcg cgacagcgtc ttgaacgtca ccgccgtgac
     9061 gaaccgctgc gcgtcggccg tcatcaccac ccgcaccgcg caaccctttt gggtcagcaa
     9121 gctcgccagc tcaacggctt tggccgccgc aatcgaaccg gtcactccca ggacaatgtt
     9181 attttctgtg ctcatcgggt ttaaagctcg ggggccggcg cccgcgtcga gcgcagcttc
     9241 tcggcctcca gaatcgtcag catccgccgc aaatcctccg ggatggtggc gctgatcagc
     9301 aaataatcaa agtgccgcca ttgggcgatc tcctgccgcg ccacgcccag gcgcttctgg
     9361 atcaccgcct ccgcgtccgt gccgcgcttg cgcaggcgcc cctccaggct cgccatcgaa
     9421 ggcggcgtca aaaagaccga gaccagcgcg cggctcaatt ccgggtcctc ccgcgcccgc
     9481 tcgcggatcg tcgccgcacc ctgcacgtcc acgttgagca gcacatcctg gccctgccgc
     9541 agcttgcccc gcacctccga cttcagcgtg ccgtagctgt agccgtaaac cgtggcgtgc
     9601 tccagaaagt gccccgcctg cacccgcttg agaaacgccg ccgcgtccaa aaagtagtaa
     9661 tccaccccct cgcgctcgcc ctcccgcggc ggccgcgtcg tgcaggtcac cacccgcgtc
     9721 atccgcggcc gcgcggcgag caactgctgg cacagcgtcg ttttgcccgc gcccgacggc
     9781 gcggagatga ccaccagcaa cgggctcgac gcgggggaca ggccggcggc gctgcccgcg
     9841 ccggactcgg ggccgggcgc gcccggacgg ggttcgtggt tttcgccatt catcattcta
     9901 cattctgcgc ctgctcgcgg aacttttcca actccgcttt gagcgtcacg acttcgcgcg
     9961 aaatcaagcg gtcattggcc ttggcgccga tggtgttgat ctcgcggttg atttcctgcg
    10021 ccagaaaatc cagcatccgg cccaccggct ccttcgcttt ccggccatcc tcgaactgca
    10081 tgaaatggct ttgcagccgg gtcagctcct ccgaaatgtc cgcgcggtcg gcgaaataga
    10141 ccacctcctt gagcaggcgc tcgtcattcg caacgggcgc ctccagcccg gcgctcttga
    10201 ttcgttccag tagttgccgc cggtaatgcc gggcgaccat gggcgcgcgc gcggcaatgc
    10261 gggccgcggc cttccgcatc accgccaccc gcttcgccag gtcccgcgcc aggtgcgccc
    10321 cctcgcgctc ccgcatcttc accatttgcg ccagggcccg cttgagcgcc ttctgcaccg
    10381 ccggccaaaa atcctcctcc accgccagat cctcatccgt ctggaatacc cccggcgccc
    10441 gcgtcacctg gtccagcgtg accgggcccg ccagcccgag ctgccgcgcc aggcgggtca
    10501 actcgcgcgc atacgcgcgg gccagcggca cattcaaacg cacccgcggc gccgccgggc
    10561 ccgcgcccgc atgcagagaa acccgcgccg tcagccgccc ccgcgccacg cagccgctga
    10621 tcacctcgcg aatctgcgcc tccagcaact ccaattgccg cggcaggctg atggcgattt
    10681 ccgactgctt ccggttcacc gaactgatct ccacggtcac cgtgaagccg tccttcgagc
    10741 aatccccgcg cccataaccg gtcattgatt tcattgccgc cagtatagaa aatcctccag
    10801 aaactggcta atcaattcta tctccgcccc gccgcatctc ccggcctccg cagactcgcc
    10861 gccgggccta aaccgggctg attcagattt cgcatcccat tcggccctgc gaaacgctca
    10921 agtccctgac tttgcgggta aaagacattc ggaacccggc ggccagcccc tggaaccgcc
    10981 agcttaacag ctcgttgagg ccaaccgccg gcctgccaag cccgcttcgg cctcagtaga
    11041 agaaggaatc gcggttgaaa cgcataacgt caacgtttca gataaggcct tctcgaagcc
    11101 aaaagccatc cggacacccg cccgcccttg aaaaaccgcc ttttgtcatc cctggcttca
    11161 taactgactg cttttacaga gcttggcaat tatccctcca tcccctctct tcgtttcggc
    11221 ctttccaggc atccccatcc actatccaga taccttggaa ataccctgcc atcgcgccat
    11281 gtacaagggg tgtacaaggg atgcccatcg ggtgtaccag ctaaaatccc atggactccc
    11341 ggtgggcatc ccttgtacac cccttgcaca cggcctgggg gcagggtaag atcagggctg
    11401 gtttatggcg cgtcgaagac ggcaggattc gggttttgcc gctattttag ccctctctct
    11461 gcctaaatcg ttgattttac tggcgaaagg ctcgggattc agcaaccatg cccttgaagt
    11521 tgctaaataa agggttcgcc agggctgcgg attggatggt cagggctggc cgagctccga
    11581 tggaacgagg gcggggggtt gaactatccc tcgggatcgt tacggctaaa ccgaaaggat
    11641 tcagatttta cctctattcc gttctgccac acgtgcgtaa agcactgatt ttacaagcaa
    11701 atggcgttcg aaacccaacg attatgctgt ggaattcgcc aaacaaaggg ttctttgggg
    11761 ctacggtctg ggggatctgg tttttcccgg ctccgatggc atgacgggac ccggttggac
    11821 ttctcaacgg aatcgtttcc gctcagaaga ttcgcgaggc cagctttccg cgagggtggc
    11881 ggcggcggag ctatttgtgg gttcggcttt tccgttcgcg cgtccgggtt tatagtggct
    11941 tttatagata cgaccgttat gaacagcccg gctcgttgct attttgatca taacgccacc
    12001 acggcggtcg cgcccgaggt ggtggaggcg atgctgccgt ttctgcgcga gtattggggc
    12061 aatccctcca gcagctacac tttcggacgc cagcttgccg ggccgctggg cgaagcccgg
    12121 gcccgggtgg cggcgttgat caacgccgat ccgcgcgaaa tcatcttcac cagcggcggc
    12181 accgagagca ataacgcggc gatccacagc gccctggcca cgcagccggg caagcgccat
    12241 atcgtgacca ccgccgtcga acattcggcc acgatcaagt tcgtccagct tctccagaag
    12301 cagggttacg cggtcacctt cctgccggtc agcccggatg gcgcgcttga cctggaccag
    12361 tttgaacaag ctctccggcc ggacacggcc attgcttccg tcatgtgggc caataatgag
    12421 accggccttc ttttccccgt ccaggaaatc gccgcccgct gccgccgccg gggcgtcctg
    12481 tttcacaccg acgccgtgca ggtcgccggc aagctcccga ttgacgcgca ggcgctggag
    12541 gcggactttt tgtccctgtc cgcgcacaag ctgcatgcgc ccaaaggcat cgggctgctt
    12601 tatttgaaac ggggggtcaa ataccagccg tacatcgtcg gcggcggaca ggagcgcggc
    12661 cggcgcgggg gcacggagaa tgtcgccaac atcgtcgcgt ttggccgggc ggcggaactg
    12721 gcgcgggcca acctggcgca ggcggagacg cgggtgcgcg cgctgcgcga ccgtttggag
    12781 gcgggcctgc tccggcagat tcccgaaacg gtccgcaacg gcgcccggga accgcgcctg
    12841 cccaacacca gcaacctggc tttcgcgggg gtcgaggcgg aggggatttt gctgctgctg
    12901 gaccaggcgg gcatttgcgc ctccagcggg tctgcctgca ccaccggctc gctggagcct
    12961 tcgcatgtgc tgacggcgat gggctgcagc gcggcgcgcg cccgcggcag catccgtttc
    13021 agcctgggca tcgccaatac cgcggcggag gtggactacg cgctggagcg cctgcccggc
    13081 atcatcgccc ggctgcgcgc ccttacgccg gcttagcgcc gccgcgccgc ggagtttttg
    13141 ctttcaatcg ggcgggccgg cgtgttccta tgggcgcgcg tatgagacct ccgtccttga
    13201 tgaccgccgt ccggttgctg gcgctggcgg ccctgctggc gccgctcgcc ggttgtgttt
    13261 ccacgccccc gccggagcaa cgcgcccggc tggagcgcgc ccgcgcgctg cacggcacgc
    13321 gggccaccta ctgccgcccg ccgcgcaaag cggacaaccg agtggacgtg gatcgcctgg
    13381 tggccgaact ggtcgaactc cgcgccaata cgtacagctt ctgcatccat aattatccca
    13441 ccgattggga ggatttgaag ctcctcctgc cgcgcgcccg ggaaaagggg attcgcctct
    13501 gggcctcgct ggtgccgccc tccgaatcgc cgccgcatac gcgccactat tccgagccct
    13561 tccggctcga ttaccagcag tgggcggtgg agttcgcgaa gctcagcctg cgggaaacca
    13621 acctggtggc ctggagcatt gatgacttca cccacaatct gaaaacgccc tatacgccgg
    13681 aatatgtccg gcaaatgctc gaggccgccc gcgcggtcaa cccgcgcctg gcctttgcgc
    13741 cctgctgcta cttcaccggg attacccggc ccggcttcgt caccaattat tgccatctgc
    13801 tggacggcat tctgtttccc tatcggcatg agtccgccgg gcccaacctg aaagaccccg
    13861 gcctggtcga gtctgaattg cagcagctca aggcgttggt cggccccgat ttccccatcg
    13921 tcctcgatat ctacgcctcc gcccacagcc gcctgggggc gacgactccg gaatacgtcg
    13981 agcaggccat gcttgccggc cgccgggccg ctgatggggt catgatctat tgccaccagg
    14041 atccggcgaa aaaccccgaa aagtatcaga tcatcaaacg gctcttcacc cgctgggcgg
    14101 cggagaagga gcggcggccg cggcgatagc cccggcattc gcggggggcc ttccggccaa
    14161 gcgcgtcaac ggcgggccgg ataattcacg ttccgggagg aacgggcggc gcgcgccgcc
    14221 ggcgggccgg gggctcgtca gcgccgctcg cttcgcgtct tcccgatgac gacgcccgcg
    14281 cgggtggggg cggcaaggcc gcgctcgacg agggttgccg gtgagactat tcttcccgtt
    14341 atgactgcca tagccaccga tgcggtgccg gacgcgcgcg gccactttgg cccttacggg
    14401 ggccggtatg tgccggaaac gctcatgcac ccgctgcagg aattggaggc cgagtatgcc
    14461 cgcgcgcggc gggatccggg ctttcagcgc gagttcgagt attacctgcg ggagttttgc
    14521 ggccggccga cgccccttta ttttgccgag cgcctcaccc gcgagctggg cggggcgcaa
    14581 atctatctca agcgcgagga cctgctccac accggcgccc acaagatcaa caactgcatc
    14641 ggccagattc tggtggcccg gcgcatgggc aagacgcgga tcatcgccga gaccggcgcc
    14701 gggcagcacg gagttgccac cgcgaccgtg gcggccatgt tcgggctgaa atgcgtcatc
    14761 tatatggggg cggtggattg cgagcggcag gcgctcaacg tcctgcggat gaaaatgctc
    14821 ggcgccgaag tggtccccgt caaagccggc cagcagacgc tgaaagaggc catcaacgaa
    14881 gccatgcgcg actgggtgac ccacatccgg acgacccatt acatcctggg cacggtttat
    14941 ggggcgcacc cctatccgct gatggtgcgc aattttcagc gcatcatcgg cgatgaagcc
    15001 cgccgccaga ttctggaaaa ggcccaccgc ctgcccgact ggctggttgc ctgcgtgggg
    15061 ggcggctcga acgcgatggg gctcttttat cccttcctga acgatgaatc ggtaaagatg
    15121 gtcggcgtgg aggccggcgg ggaagggata gccacgggca aacatgccgc ccgctttcaa
    15181 ggcggctcgc tgggggttct gcagggcgcc tgctcttacc tgctccagga tgagcagggg
    15241 cagattcagc ttacccatag cgtttcggcc ggcctggact acgccgccgt cggccccgag
    15301 cacgcctggc tgcgggattg ccggcgcgtc gaatataccg ccgccacgga tgcccaggcc
    15361 ctcaacgcct ttaccgagct ggcccgcctg gagggcatca ttcccgccct cgaaagcgcc
    15421 cacgcagtag ccgaagtcat ccgccgcgcc cccacgcttt cccgggagca aatcatcgtg
    15481 gtcaacctct ccggccgggg cgataaggac gtggcgcagg ccgcccaatt catctccttc
    15541 tgaaacggcg ctcccggcgg agccgccgcg cgggcgcgcc ccggacggct tgggggaacg
    15601 gggcgggcgg gcatcggagt tttttttgat ttatatcaag gcggaaccgc cgaaaaggtg
    15661 taggttgcgg gtatgagtga acaaccttta ttggatgcta aaaacgagcg gattatctcg
    15721 ctgaccaaag aaacgcagtt tgccgccaac ggcatcgtca gccgcacgct gctgcggacg
    15781 gatgtcgcgc aggtgacgct gtttgggttt gcggaagggc aggaactgac cgagcacact
    15841 tcgacgcaac atgcgctggt gcagatttta tcgggcgagt gcgaattctc cctgcaagga
    15901 aagccgaaca ttctaaagcc cggcgatttc ttctacatgc cgccgaatct gccgcacgcc
    15961 gtgaaggcga cgaaaccgtt ttcgatgctg ctgacgctgt ttaagccgtc caaagcctga
    16021 cccgccggtt ttgatcctaa cgccgcgcgc gggcgcgcgg ccggttgtgg gacgctggca
    16081 acgccgcgtg aaagaatgcg gcgggcgggc gtcctttttt gttttgccgg ccgcgccggg
    16141 gggagacgcc agcggttagc agccgcgccc gctttcccgc gcgaggcggg agaacgtctc
    16201 cgcctgctgc cgccgcaccg ggtcttcctc cagttcccgg acggtgtggg ggaggcggta
    16261 agtccagttg gtctgggcga cggtgccggg ggtgttgaag cgggcggtca tggcgaacag
    16321 gtcggtgatc tggaagacgg ccagccagga agcggactgg aggacggcgc gggaataggc
    16381 ttcgtgcagt tggccggcga actcacgcgg cggctcgatg tcccgcaagc cggcaaaggc
    16441 cataatctgg cgcagctcgc ggcggctggc gtcggcttcc gtgccggcgt tgatggcctg
    16501 ccaaagcccg tcccaggcgg cggccagcgg cgggtgatca tgcgtggcgg gctggacgag
    16561 cgacagccgc ggataccgct tcgggtccgc ataggcgccg tcgggctcgc ggaaaaacat
    16621 ggggatgcga aagccgggaa tgccgagctt gagcaaggtg ggcggcacgt agtcgggcac
    16681 cacgcccagg tcttcggcca ccacagtggt ctcgcccgag ccggcgagga cgacccggag
    16741 gatttcctcg ccctgggcct ggttgaaggc cttgtgttcg ggggtgtcgt cggggaatgg
    16801 cttgaatccg ggcaggcgcc cgccggtgcg ggcggcggct tcggcctcgg tcagcggcag
    16861 gaactccgca ttgcggtcgg gcgtccacgg aaaggagtag atgcggaaga agccgagcac
    16921 atgatcaatg cggtagaggt ggaagagctt ttggaggttg ccgacgcggg tgcgccacca
    16981 gccgaactgg cggcggcgca gttcctccca gcgatagttg gggatgcccc agttttggcc
    17041 ccacttcacg gtgaaggggt ctgccttgaa ggtcgtttcc ggcggcgcgc cgccgctcca
    17101 gtccaggtcg aagaggtcgc ggttggccca gacatccgcg ctataacggc ccacgccgaa
    17161 cgggatatcg cccatcagat agacccgccg cgccgcgccg caggccttga gcgcctgcca
    17221 ctgggtgaac gcgagccatt gcacgtattg aaagaaaagc tgccggcgcg ccaattcctg
    17281 gcggcgctcc cggggcagcg accgcagcca gtcgcgggcg gcggcgggcg aatgatgttc
    17341 gggcggccag cgatcccagg cgggcgtatt cccgttttcc tccactagcg cgcggaacag
    17401 ggcgtaatcg tccagccagg cgcggttctc cgccgcgaag gcgtggaact ggcggccgcg
    17461 ttcgtccggc ggatcgagat gctgcgcggc gaaatgcgcg aaggcggctt cgagcagccg
    17521 gtgtttaagc gcccggacct ccggataatt gaccggcccg cggcgcaacc cggccacccg
    17581 atccggcggc gccagccgca ggaaggtttc cggcgtcagg tccgggaggt ggcgcggcga
    17641 cagggcgagg gtggagggtt cgagcgccag cgagctcagg gcgttgtaag ggctgttgtc
    17701 attgcccagc tcgttgatgg gcaggatttg gaggatgttc aggcgatggc ggtggcacca
    17761 gtcaatcatc tgccgcacac cctcggtatc gccaatgccg aggtcctcct cggtgcgaat
    17821 ggcgaacacc ggttccagaa tgccggccag cggctgggag gggcttggtt tcatgccgcc
    17881 ggcccggagc cggcccgcgg ctcgacgtaa cggacttccg ccgggagccc caggcgggcc
    17941 agcaattcgg cttcgatgct gcgcttcggg tcatcggcgg gccacatggc gctggcgaag
    18001 tagtcttcgg agatttcgag ggcgatttgc agttgccgtt cggagggagt gaggtggaaa
    18061 cgaaggcccg ccgcgcgggt ttgggcgagc acttcggcca cctgggtttc gtaaaaggag
    18121 atttcccgga cctggtggcg gtggtccagc cgggggccgg gctgaaacag gacgccggtg
    18181 cggccgcagg cgcattggtc gtggcaaagc tcggcggcga cgcgcgtgcg gtagcggagc
    18241 aggggcatgg cttcgcgcgc cagggtggtg atgaccagtt cgccctcgca gacctccgcc
    18301 aggaattggt cttcgtggat gtgaaacaac cccacctcgc actcgacgca aaggccgggg
    18361 tcgaggattt cacccaggcc gaagttgcag cggacggcgg cgaagaggcc cgaggcgatt
    18421 tgctcgcggg tttcgcggtc cacgggccgg gacaacagga cggtgcgcag gtagagggac
    18481 tgggggtcca cgcggcgttg ctcgatggcg cggatcaggt ccagcgcgtt ggtgggggtg
    18541 gtgatgagcg tggtggggcg atagttctgg agcatcgcga gctggtaatc tatatgcagc
    18601 gggtcttcgg cgatgaccga ggcttcgatc aactccgccc cccgcacata gcccacggcg
    18661 ccggactgcg tcaggccgcc cagggaaatc aggatgacgt cgttggcggt gacgccgctg
    18721 gcggagagtt ggcgggccac cagccggctc cagagggcca ggtcgttgcg ggtgtggccg
    18781 atcaccagcg gtttgccgcc cggcccgagg gtcgagtgca ggcgaatcac ctcgcgcagc
    18841 gggaaggcga acatgccata cgggaagctc tcgaccatat cctcctgcgt ggtgaaaggc
    18901 agccggggca ggtcggccag ggattggagg cggaagtcgc ccagcttctc gcggtagcgg
    18961 cggacattgc gccgcagccg ggccagcagg gcctgaagac gctccaactg catctgctcc
    19021 agttcggagc gggaaatctg ttcgtagcgg cggtcaaacg tgctcatagg agaataaaac
    19081 ggcagcgggg gcttcagcct ttgccgaggt aggcccgttt cacctcgggg ttgtgcgcca
    19141 ggtcggcggc ggggccttcg agaacgattt cgccggtttc catgacgtag gcgcggtcgg
    19201 caatccggag ggcggcgttg gcgttctgct cgatcagcag cacggtcagg ccaaggtctt
    19261 tcatcttgcg aatggaggtg aaaacctctt tgaccatgag cggcgccagg cccatggaag
    19321 gttcgtccag cagcagtaaa cgggggcgcg ccatgagggc gcggcccagg gccagcattt
    19381 gctgttcgcc gcccgaaagc gtgccggcca cttgctggag gcgctcgcgg aggcgtggaa
    19441 acccgtccag cacggtctcc aggtgccgga tgcggtcggg gttatggtag ccgccgatga
    19501 tcaggttctc gcggacggtc atatcggcaa agatcatctt gccttcgggc accagcaccg
    19561 tcgagttgct gacgctgcgc cagggcggct ggccggtggt gggccgccgg tcaatgaaca
    19621 cctcgcccgc ccagggtggc agcaggccga tgatcgcctt gagcagggag gacttgccgg
    19681 cgccattggc cccgagcagg ccgacgatct cgccgtggca aacgtgcagg ctggcgccgt
    19741 gaaccacctc gaggctgcca tagccgcagc ttagattcct tacctggagc agcatgtcgc
    19801 aggcggggtg gcgggagcgc gcgggggcgc gtctccgccg gattcatcgt tttgcaggtc
    19861 ttcgcccaaa tacacgcgga tcacccgggg atcattttgg acggccgccg gcgcgccttc
    19921 ggcaatgggg cgtccgttgt ataatacgag gagatcgtcc gaaagcccca tcaccaccga
    19981 catgtcatgc tcgacaagca gcacggtgat gccccgatcc cggatctggc ggatcaggcg
    20041 cccgaggtct tccttctcgc gcgtgttcaa accgctggcg ggttcgtcca gcagcagcat
    20101 cgccggctcg gtcgccagcg cgcgcgccag ttcgaccatc cggcgctggc cgaacggcag
    20161 cgcgcccgcc gaccagcccg ccaggtgcgc caggcccacc acgtccaact gcgcccgcgc
    20221 cgcctcgcgg atggcgcgct cttcccgggc ctggcccggc cagcgcaagc cgcagcccag
    20281 gaacccccag cgcgtgcggc aatggcggcc caccatcacg ttctccagca cgttcatctg
    20341 gaggaacagg ctgggatttt ggaacgtgcg ggacaagccg gcgccggcga tttcgtaagg
    20401 tttcaagccg gtgatcgagc ggccccggaa ggtgatccgg cccgcatccg gtttgagcag
    20461 gccggagatg aggttgaaca gcgttgtctt gcccgcgcca ttggggccga tgatgccctt
    20521 gatctggccg ggtttaacgg tgaaggagac cccatccacc gccgccaggc cgccgaaggc
    20581 gcggcggaca tccgtgactt caaggagcgc catgcggggt cctcctttcg gggtcgggcc
    20641 ggccagccag gcgccggagc cacaggccca ggggcttcag cgggccttcc ggcgccaggg
    20701 agatgatcag aatcagaagc gcgccgaaga cggcgttatc gtagctcccg aaccagccgc
    20761 gcagggagca ataattcaaa gcgatgctca tcaccgtcac gccccagaga ttggccatgc
    20821 cgcccgcggc ggccagcgcc acatagcgga cggacttcag cgccccggct tccgacgggg
    20881 caatcccgcc ggtatagtgg gtaaagaaca cgccggccag cgccgccagc agggcgctca
    20941 ggacgaagac cttgagcttg taggcggcgg tgttgatgcc cattgcgttg gccgcggttt
    21001 cgcggtcgtg gatcgccagc agcgccctcc cgacgcggga gcggagcagg ttgcgcaagc
    21061 ccagcaacag cagcagcgcc aggccgcagg cgaggtagta gttctggacg cgcagggcgc
    21121 tccggccgga gacggtcagc cccggcagca gcttccactc cggcacgccg tggatgccat
    21181 ccgccgatcc ggtgagggcg ctgcccagca gcagcttgct gacgatcagg gtaaagccga
    21241 gcgtagccat cgccaaataa tgcccgcgca ggcgcagcgc cgggtaaccg atcaacaacg
    21301 ccaccacaaa cgtaaggagc atcgccgccg caaacgcggc ccacggcgcg acggacagcc
    21361 ccgcctggcc atataaatcc tgctgggcga ccaacaggcc cgcccgctcc aatacgccca
    21421 ccccggcgca ggcccggatg ccggagaaat catgcgtggt cagaaccgcc gtggtatagc
    21481 cgcccagggc gaagaacgcg ccgtgcccca gcgatacctg ccccgcatag cccatcagca
    21541 ggcaaagccc catgaccacc agggcataat aaaccgacat ggtgagctgc gtcaggcagt
    21601 actccatgcc cgccgcgcgc aggccaagct gcgcgcccgc caccatcacc gggaacaggg
    21661 cgagcaggaa cagcaagcgc aggcgaggca tggccgtggg gcgtcaggcg gacggcggct
    21721 ccgccggggg gcggaggccg ccggcgcggt cggggaggat gatcgtatga cgcatttgat
    21781 gagttgcggt tctaatgttc gcgaacgcgg tccggcgcca gcgcgcccca caagccttgc
    21841 ggccgcacga agatcaccag cagcagaatg acaaacgcgg agacatcgtt atagaccgcg
    21901 ggcaggcaac tcacgctggc cgcctccagc aatcccacca gcaacccgcc ggccaccgcc
    21961 gccatgggat tgcccaaccc gcccaggatg gcggccgcga agcccttgat ggccagcggc
    22021 gcgcccatct catagtgagt catggtcatg ggggaaacca cgcagccggc cagcgcgccc
    22081 agggcggcgc tcagcccaaa ggcgagcgtg cgcatgttgg gaatgttaat gccggccagc
    22141 atcgccgcct cggggttcga cgagcacgcc cgcatcgccc ggcccgtcag ggtatagcgc
    22201 agaaagaaat gcaatcccag caccgccagc gccaccgtac ccagcaccca cagcacctgc
    22261 gtggaaatcg ccgcacccag aaagcggacg gacgacacct cgttgccggt gaaataagga
    22321 agcgaacgca ccttttcgtc ccagatatgc agcgccgttt cctggatgac aatggacagg
    22381 cccatggtga tgatgaccag gtgcaggatg gagtggcggc gcagccggcg aaaaaacacc
    22441 aactcaagca ggccgcccac cagggcgacg agcgccaccg cgcccgtgac cgccaccggc
    22501 agcggcacat acgccgccag cgtgaccgcc accatcccac ccaacatcag gaactcgccc
    22561 tgcgcgaagt tgatgatgcc cgtcgcgctg tagatcaggt tgaacccgat cgccaccacg
    22621 gcgtagatgc tgcccttggt gatgcccgac agcaggtact gcagcgctaa gctttcgttt
    22681 tgcacgcgcc gggcattaac ccaaaaaccc cggccggcag accggccggg gcgcaaagcc
    22741 gtcgtaatca gtcggcatgt tcggcgctac tgcttatacg cgacgaattg accgtctttc
    22801 accgtcagca tctcgaaggc cgtcaaatcc aggccggtat ggtcggtggg ggaaaagttg
    22861 aatattccgg cggtgcccac gacgcccgtc agggtctcca gcgcgtcccg caccttttca
    22921 cgctccggtt ccccggcttt cttgagcgcc tccaccacaa ccaggagcgc gtcataagcg
    22981 tgcccgccaa aggtgctgac gtcttccttg aagcggcttt cgtaatcctt cttgtatgtg
    23041 gccagcactt ccttttgcgg atgattggcc ggcaaatcct ccaccaccag caaccggctg
    23101 gcggggaaaa ggatgccttc cgccgccgcc ccggcttgct gcacatattt gcggttgcca
    23161 aacccgtggc tctggaacag cggcatgtcc atgcccagtt gcttcatatt cttggccacg
    23221 atggactggg ccggcacgat ggaccagttg accaccgcct gcaccttggg gttgcccttg
    23281 atcttggtca gcacgtcggt caggtcggtc gcctgcttgt cgtacacctc gttggccacg
    23341 atggtgatgc cctccgcggg cgccaggtcc tcaagctgct tcttgcccgc cgcgccaaaa
    23401 ccgtccgtgc tgctcacaat ggcgatgttc gagatgccct tctctttcat cgtgcggtaa
    23461 atccaggtcg ccgcctggct gtccttctgg ggcactttga acacataccg ggccacgggg
    23521 ttgacgatgg tttccgccgc cgcgcaggac accagcagca tctggttctg ctcgcataaa
    23581 tccttgatcg ccatggtctc gccgctggtg gacgggccaa gaatggccag cactttctct
    23641 tcttcgatca actgctttgc aaacgacacc gccttttcgg ggctgccggc ggtgtctttg
    23701 accaccaact gcagcttccg gccattgatg ccgcccgcgg cgttgatctg ctcgatgcgc
    23761 atctgcaccg tcttctcttc cggggcgccc aagaaggacg ccggcccggt cacggaaaat
    23821 aacgcgccaa tcttgacggg ttccgagggc ggcggaggag aacagctcga ggtaagccac
    23881 aaggtgccaa gcacaccgat tcccgccatt acaacgaaga ttcttttcat gtcagttcat
    23941 ctttctcttt gatcaacctg cgattgcccg gccgggctgg ccccctttgg gggattcccc
    24001 aaccagtcaa acgcaggttg acccaaaatc cccgccggtg tcaatgcaaa accccctccc
    24061 gttttacttg cgcccgcgct tcgctttggt tcatctgcgc cgccggctac ctgatttccg
    24121 gcagcaaccg gctggcgccc gaaacgtgct gcccgcccct gatgcggcct ctttgctgca
    24181 agccaagggg gcacagcccg cgccagccaa cccggtatcg caggtttttg taatcgccag
    24241 acggcaacgg tgccaaagcc aatcagtcgc gccaatcatg ccggacctcg tgtttggcgt
    24301 gccgttgcca caccacgaac ccacggtgca taacccgaat aacctgtccc atgcgtttgg
    24361 attgggagtt gaagcccggc tgccgcagca tccggcgaat gaagtgcgcc gcctcatact
    24421 gccccaccgc cagaatcacc agcgagtatc gcgcgtgaca caactgccgg gaccagaaat
    24481 cgaggtcacg cgtgaagaaa gtgggccggc gcaatgtcag cagaaacggg ataatttccc
    24541 cgtcgcacat tcccttccag ccgagttcgt gcccgatctg ccggaccgga atgcgccagc
    24601 cgagcaagga cagccgctgg ctttcgagga tattctcatc caggacgttc acacccttgc
    24661 gggttccagt cccagttcat gcgcgtgttc gcgaaacgtc tcgccggcga gccgcaccgc
    24721 ttcggctatc gcttcccggg tgatgctgcc cctccattcc tcgacgatgc tctcccaggc
    24781 gatgccgcca gccacctgct ccaatacgtc ggccaccaga atccgcgttc ctcggaaggt
    24841 cggctcgccg tggcacacct ttgggtcgga cacaatataa cggcctacca gcttcttctt
    24901 cattgctgcc ccaccaatac cccacccgct tcgtcccgtc aaccgatctg ccgccgtctg
    24961 ccggtaggct caggcaagaa cgcctcgtca tggcatcggg ttgttccggt tggcggcggt
    25021 cccagcggca tagcgtattc ccaacgcacc gttcccggcg cgcatcattt gccgtccatt
    25081 tagccacatg caaaaaacca gcaagttctt gagcgctcgc gaggacgctc acccttctgg
    25141 caaaaggttt aacccacccg ccccggacga gcggtttagc caacatccct ggacgcgccg
    25201 tttactcgct ctgaatgcga tagtacttgg ctggcgccgc gcccaccgcc gggatggtga
    25261 acgttcccgg ggtggcgaag ttagtctgca cccgggccca actgcccagg ggcgccgcta
    25321 catccgacgc ctccagcagc acaaactgcg cgccgccgcc gccgccatag ctgagggtgg
    25381 ttccctgcag gcggctgatg gtgacgggag cgacggtttc cgggccgatg gggtcgccgt
    25441 aaacgcgcac catatcgaac cgaatggtgc cggccgggct gtaggtgctc tccgtgccga
    25501 cgtagttcgg gttggccggg ctctcgaact cggtgacgat gcggaaggca aagttgggat
    25561 tattatgggc cgccggccag gtggccagat cgcacgcgag cgaaacgaac gtctcgccat
    25621 tggtcatcgt attgaaatcc aggtcgctaa aggtctcgcc atcggcgctg tattggaggc
    25681 gggtgtattt gctgccggta ttgctgtggc gctggctcca ggaaagataa atattctggt
    25741 aaccgagcgt gcttacgttg aactgcaccc cggcggtttt attgccgctg ccctgggatg
    25801 gatagctcga ggtattccag gcctggttgg tccccgtcgg tctgggatcg gacgacacgc
    25861 cggcggcgta ggttgcctta accccgccga gcagcgccgc agtgccggcg cccaacgagg
    25921 gagtcggcga cgccaccgga gcgttggtgt cattaaagtc ccacagcgcc agcgtgacgc
    25981 tggggaaccg cgtgacggta agctggatgg gggcgctggc gacgtagtcc ccgaggccgt
    26041 tggtcaccca gcaggtgtaa gcgccctggt gggtgatttg caggtcggtg agcgtcaggg
    26101 cgctgcgggt tgccccggca atgggggcgc cgccgaagaa ccactggtaa ataagcgtcg
    26161 gcgtgccgcg ggcggtgacc gtaaaggtaa cgttcgttcc ttccgggcag gtgcgatcgg
    26221 ccgactggga ctggatggca ggatccagca cggtcaccgt tgcgaccgcg ctggtagcgg
    26281 cgcccgcaga gttggcgacg atgacgtcgt agtcgccggc gttggcggcc aggacgttgg
    26341 tcagggtgag cgtcggcgtg gtgctgccgg aaagattgcc ggcgtcgctc aaggccgcgc
    26401 cgtctttgcg ccactggtag gtgagcgatt gggaggggga gccatcgctc gtggcgatca
    26461 ggagaaccgt cgccccggcc tggttggttg gcgccgacag ggccaccgtg gcataggtgg
    26521 gcgcgctggt cgagatcgtc ccgctgctga aggtcacgct caagtcgtca atcgccatcg
    26581 cgtggtcggt gccggtgtca tcaacatccc gccagcgcag gaaaagctcc gcgcccgccg
    26641 gcaacacaac tccggtcagg agtatatcgg taaactcctg ccgatgggcg gcgtcgtttc
    26701 cactcacggc cgacgccccg ccgccaacgg tgggcgagat gaaatccagg gcggtcaacg
    26761 gagtccaggt gtcggcattg gtcggatcgg ggctggtgat gggatcggtg ctgacgcggc
    26821 aggagcaagc cagggtctgc gcagtggtat tgccgccgtt gcgccactgc tcgccggtgt
    26881 aggtaatcgt aatgctggca atgtccgtgc cggtgtcgtt ggcaaaacga acgccatagg
    26941 cgatattgcc taccttgtca tttccggagg cgagggagcc caaagcccgc tccggcgacc
    27001 cggtggcgcc gaaactataa agcgctcccg cgttatcgct gccggtgccg gcggtatact
    27061 tcgcaaaact gtcgggcgtg cctttattgg atttccccgc ataccacccg ggcagggtcg
    27121 cgttgtcctg ccaggggtta ttggcgccgg aaatggccag cgaattaaag ttctgactat
    27181 agacgccgcc cggcagggaa ctgatttgga tttgggccga agccggttca agcagcaagc
    27241 ccgcgcccag gagcacggtc aacccgataa cagagcgaag tgttcttttc atggttatga
    27301 ttggcccttt cccgcgctcc gacaaccggg accggggaaa ggcgttttta gttcctattg
    27361 taatcagcct ggcaggcagc cgccacccag ccgccgcccg ctcaacctgc gttaacgatg
    27421 atgtccgcag actatgcgga ttcccatccg cagtcaatcc aaatccgcaa aaaaacaagc
    27481 cccccgggcg cgccccggtt ggcgggcggc cggttccggg gaggcctcgg gtgatgttgg
    27541 atttgcgcgt ggcgtgaaga tgagcgagag aatacagaca tgttgacgac cgtagagttg
    27601 atgcccgaag cccccccggc ggaccgggtg ggcaaagtgc tgccgcgctg gctgcgcgag
    27661 gtgccccttt atcagcgggc ggccaagctg ctgccgttga acggcaagcc cctcgccctg
    27721 gaggatttgc gccgcctgcc cctgattacc aagcacgaca ttcgccgcga cttcccgcgc
    27781 aatttcctgc gcgccggggt cgagctcgac acgctgctcg atcaggacct ggtcgaactg
    27841 gagcaaacat ccggcacctc ggaggagcgg gtgccgctgt tgctggggcg gggctggtgg
    27901 gcggagcagg aggagcgggc gctgcggctc aacccgctgg ccgcccgggt gctggacgac
    27961 tttcccgcgg cgcggcgcgt gacgatcact tcgccggtgt gcagcgggga tatttgctat
    28021 tcgggcgtcc ccacgcacgc cgaccgcatc gtgggcaacg cgctgtttgt cagcctctcg
    28081 cggtacccgt ttctctggag cgacgccgag ctggcgcgca tggccggcga agccgcggac
    28141 tggcagccgc agtttctgga cgtggacccg gtttacggcg ttctgtttgc gctctactgc
    28201 gagcggcagc gcatccggct gccgtcgctc cggttcattc tgagcagcta cgaattcgtc
    28261 agcgccgccc accggcgcat cctgcagcgc gtgttcggcg tgccggtgct caatctctac
    28321 ggctcgaccg agaccggcca tctgctgatg gaaaccggcc cggatgaaat gcggcccagc
    28381 caggaaaccg cgctgctgga aatggtcgcc gccgacgagc agggcatcgg cgacctggtc
    28441 gtcaccaccc tcaccaacga tttcatgccg ctgatccgct accgcattgg cgacctggtc
    28501 gaacagctcg gcccgccccg cgccccgcgc tacgtggtgc atggccgcgc ggcggacgcg
    28561 ttcttcacgc cggccggccg ccgcgtgacg acctggcaga ttgaccagtg cctggccgac
    28621 ctgccgggca tggttcacta ccagttgtgc gagcagccgg acggcggctg gttgctgcgg
    28681 ttcgtgcccg atcccgcggc gccggacgcc gccccgccgg cggagctcca acgccgcctg
    28741 gtcgagcggc tggagattcg cggccagttg gtggtgcagg caacggattt gctggtgccg
    28801 gagcgatccg gaaagttccg gctgggatac ccggccagaa aacccactcc ggcgccctga
    28861 gaccgcccgg cgccgtccgg cggtggaggc ttcccgggct aaggcgtccg ggccgggcgg
    28921 aggcgttgcg cctggcgcag gcaaaaatcc agcccctcgg caacgccgtt gccgtgcgcg
    28981 agctggctga caaagccttt ttgccgccgc acggcatctt tcaccgggtc caccgcgttg
    29041 cccggcaccg ccagccagtg ggcatattgc cgggaaagca tcggcaaatc gtttagctga
    29101 tccccggcgg caaaaacctc tgcggggccg agctgcagcc ggcgggtcag ctcggccagg
    29161 gcggtgcctt tatcgtatcc cacatgatga aagcgggcat aaacatcatt gcgcataagc
    29221 ccgaggttgg gaatgccccg gcaatactcg gccagatagg cgtggatggc atcggcatcg
    29281 ccattattgc cggcaatcaa acagaacggg gaataggtgt cttcgtagag ccgcgccgga
    29341 aagcgggcgc tgatccagcc gatcaggcgg ggcaaatcct gccgcacccg agcaaacagt
    29401 tccgcatggt cgcgcgcgca ggcggcgttc cactccgcga gccccaggta ctgcgaatcc
    29461 gcgcgctggt aaatctcgcg ttcgaccagc accaggtaat ccggctcgat gcctatccgc
    29521 gcgcgcgcca gcgcctccat gaggctggac aggtcgcgcc cggtgttgat cacccagcgg
    29581 gcgccgcgct cctgcagctc gccgatgcgc tgctgcacgg acgccggaat cggcgggctt
    29641 tcaaactcgg caaagagcgt gccgtcgaaa tcggttgaaa taagtttaat cggcaaacac
    29701 atggtcatgg cgcgggcggg cggcgaaacc ccggttcccc cgccgcgcgc ttgcgccggg
    29761 acaggatgct gggttaggct ggcttgtcaa gattaccatg aagaccgcct acgaactggc
    29821 catggaacgg ctgggcaaaa ctgcgcctcc cgtgaagctg acggctgccc aaaaacggga
    29881 aatcgccgag ctcgaagccc aatgcaccgc caaaatcgcc gaacgcgagc tgctcctcaa
    29941 aggcgagatc gcccgggcga ttgataaagg cgacgccgag gccctggagc agttggaaaa
    30001 acaactggtc agcgaccgca aaacgctccg ggccgagtgc gaggcgaaaa aagaacaggt
    30061 gcgaaaagcc gctgggtaag cccggcctgc ggcgccggac aagccccggc gttcccgggc
    30121 ggtgacgctc aagccccgcg aagaggcccg tcctccggcg caatcccgcg ccgccacagg
    30181 tcgaagtaat gcagcgcggc caccaccagg gaatgccgga tgcggcccgc cgccaccaac
    30241 cgcggcaggt cggcgatggg cacccgccgg gtgatcaaat cctccccctg gtcgagttgc
    30301 acccggtgcc ggcggcggca attctccacc agcaccgtaa agcaggtgtt gctcatgatg
    30361 gccgggttcg gaaagacccg gcccagcagg cgcgggtgct ccccttcgta gccggtttcc
    30421 tcgcgcaatt cgcgcagggc cgctgcggcg ggcgaggcgt cctggggatc aatcatgccg
    30481 ccggggattt ccagctcgac cgtggtcgag ccgtgccggt actgctcgat catcaccagg
    30541 tgctggtcgg gcgtcagggc gatgacgttg acccagttga cgcaatcaat cacatacaca
    30601 tcctgggcct gccgggtgcg gggcgacacc acgcgatcgg agcggatgct gaaaatccgg
    30661 aactcgccgg ccggttgcga cccgagcttc ttccagggct tgagcgccat ctatccgggt
    30721 tcgctccctc ccgccggcaa agcctggccc tgggatggga aataatcttc cgccagcgcg
    30781 gcaaaccaga cggaggtggc ccactcgccg tggacccagc ggttcttcac aaactcgccc
    30841 tcgcgccgaa gcccggcctt ttcccacaac cggagccccg ccgcatttcg gtggtcgcac
    30901 caactcgcca cacggtgcag ctttaagccc cggaagcaaa aatccagcag cgccgccacc
    30961 gtctcccggg cgtatccctg ccgctggaac ttcctgttca gccccaggct gaagttgacc
    31021 tgccgccact gcggatccgt gaagttcaag gtggcgtagc caatcaattt gccgccgtcc
    31081 tggagttcca gccccaggaa aaacggctgg tcgggcgtgg tcagcttgac gtgcgcatcg
    31141 ctcgccagcc agcgcaatat cccgtcctcg tccagcggca cgccgtccgt atagcggaag
    31201 aattcctcat ccgccatcaa ctccagcaaa tcctgccagt cctgggccgc caaccgccga
    31261 atcaccagcc gggccgtggt gatcggcagg ggaatcgccc tccccaccgc gccgccatcg
    31321 tcgggcacaa tgaaactctc ggtacagttg gggcaggcct ggacaaaccc ggcgctctcc
    31381 cgcggaaagg aaaccggatc gccgcaatac gggcacgtaa aatcaacgaa attatcttca
    31441 gcaatcatat cgcgttccag cgcgccgcgg cccccgagga cggcgggcgc ggccagccac
    31501 agccccaaac ctgccgccgc tactcctcca gcagggcgtc catgtcgccc agcggacggg
    31561 taaaggtggc ctgggcgggc ttgatcacca gcgtctgggc cgtctccttg atgctccggg
    31621 aagccgccgc ccacggcagc gcaaccacga acaccgcgga accaatcgca gtcgccacca
    31681 ggcaggctgg acgaaccacc gccgcatcag ccaccaccgc cagcgggccg tggtcatcgg
    31741 ccgcgggaca ggaagccgct ccacccaggg tgattgccgc cacgcaaatc acagctacta
    31801 atttggctct catcacccca accttcaggg acagcgcgcg ctttgtcgaa cacattttgg
    31861 aaaaggcttg cctccggcac cggggcaggc caaaaggaag gctggcgagg ccggtcggaa
    31921 gccaaagatc gccctcgttc gggcccatag ctcagtggtt agagcaggcg actcataatc
    31981 gcttggtcgg gggttcaaat ccctctgggc ccaccatcct gcataaaaag gattttggaa
    32041 ccgccacccg ggattcctcc ccgatttggg tcaagccagt gtgggacacg cggagtttgc
    32101 tggcccccga taaaggatga taatccggtt ggcgatggag cagcagccgc gagcctttcc
    32161 tctgacggcg ctgaagacgc gattaagccc ttcggtatgg tcatcgcaga ccactcgccc
    32221 gccatctcga ggaccgcagg ccgattccca aaacgccgac cgctcccgga actccagaaa
    32281 cgccatgccg cctgcggctt caaatcagct cacggccacc tcccaattca atcccagtcc
    32341 caacaactca tgaaacaagg ctcccggtgc catacctcaa gctcgcccat cctcctgccc
    32401 tgttttatcg aattcgcgaa gaaccccccg ttttttcata ttaacagatg cgcgatgacg
    32461 acgaacaagt cgcctccgtt ctgtcaggtc accgttagtc tatcctgccg ccaagacagc
    32521 tacggcttca cgacgagttg cgcggcattg gccaaatcta tcgcgatgag cttccgggcg
    32581 cttttctcgt cgggccagcc ttcgatgttg gcatgtcgtg gcggtggttc ggaaggtgcg
    32641 accgtcaccc ttgctttgga cacatccgca gccacgatgt cgccccgtgc gtggagcgtt
    32701 tacgaccgct ttgcgccgac ggcctcgccg atcccccacg tcgccggttc atcaagaccc
    32761 ttcgtctgga agacggaggt tttgccgtcc gcgcccggca tgaaggcatt gtgcttcacg
    32821 cgctgattgc cgcggctgaa gtgcgatgcg gaataaatga agcgggccaa cgggtcttca
    32881 ggcgcgatgc ccgaaccgtt gatgccctcc ccgctcatgc gatgacccgg cggatgttgt
    32941 ccagaatcac ttggggaatc tggttcgtga aaatctccgt gtcgtgggtt tttcgggagg
    33001 ggttgaactt gcccgcgtag gacaagccgt cgttcgcgcc gatgctgacc gagaagacgc
    33061 gcccggttgg gcaataccat tccagcgaaa cctcaccgtc gggatcaacc gccacttccg
    33121 gggcggggaa attcgcgggc agcgcctcga tgaaccgttt ggcccgcaga taactgtcgt
    33181 aggaagcact cgcagcatcg tagccatccc agccgggagc accggagtcg gaaaaaggcc
    33241 gcgtggagcg cgtccaaagc gttcggtttg tgcctgcgtt tccttttcaa gaggtcacgg
    33301 cgtattccac gccccgtgga ttctctacgc gttttgaagc gttgagcact tccagcccca
    33361 ccacgttgcc atccttgtcg tagtcgagga tcacgccggg cttgtcttcg tcgctctcct
    33421 caattgcagt gtcgcgaaag aggattcgca gcacgtccac ttctgggtcg taggtcactt
    33481 tcatgttgtc gttttccagt acttgctgat cttgctcgtt cgataaaccg tcaccacttt
    33541 cattggcgtc accgtttggt tcaccatcac ccgcaccaga tacggcttct ggttgatttc
    33601 aactttcgat tgatagcata ccacatcgcc gtgttcgggc actttttggc ccggcgcatc
    33661 cagcaccgac tccacgacgg ccagcgggat gccgcgccgc tccatttcct gctgaacgtg
    33721 gcttgaaagc tcgtgtctca tggcttggac tgggtggatt cctccaccag cttgatctcg
    33781 tcggcggtga ggccgtagag gcgatagacc cgctcgtcaa tctcgcgctc cagcgccgtg
    33841 gtgtcggcgt ccggtgcggc acgcttggcg ctcaagatgc gctccaccaa actcaccagc
    33901 gcgtcgtgct cggcccgctc agatgcgttg ctgaaatcaa ccggcacgat gggcagttgt
    33961 tcgatgaact gcgtgaaata acggaagaat ccgcctcgga gtggcgtgct gatcctcctc
    34021 caataccaat ccagcacggc gctgttcagt agcgcgagga aatacttcgt tgactctctc
    34081 acaccctctc tgaaggtgat tccgtaaccg ctcgtaaatg cgaagtcacc tttctcgtcc
    34141 aaggcaaacg ccgccctatc ggcgatgtcc ggcacaagaa tcttcggcag caacatcagg
    34201 tcgatgttct gttttcgacc gaactgaaac cattcaggcc ctttgaaacg ccctccttcc
    34261 cgctggctga ggtaatgctt gttcgctcgg tagtagtcaa aagctcgcgg catagcctga
    34321 gcgagttctc gctccgaggc cagagttgca tcgccgcctt ggattcgata gggcatcagg
    34381 acaaccttgt tggaagactg gattgaatac gctttgattt cacgcccttg aagaaaacgc
    34441 aatacggcct tccgttcgac aaccacgtct tggtcgagtt gctttgagta ggcagtgatg
    34501 aggccgccct tttccgagcg gacatctagc acgtaaacct catttgcact ggtgcggatg
    34561 ccttgagcaa tccgcgtcgc gatcctggcg accttgatgg gtagggcttg gagcttgtct
    34621 tgaagcccgg cgttagtccc gacagcaaaa ttccactccg ctggtgtgaa acgggcggca
    34681 ggaagtgagg tttccggtgc gcggagggta ttgagccagt caggcaagtt gtcggcgcgt
    34741 acccagcggc acgcatctgc gcctgctctg gacaggaaca acaggcagac gtaattggtc
    34801 gcgcccggaa aaatctgctg gtcgccgaag tggacgacgt gagacagatg ccgtcccttg
    34861 gcaagctgcg cccgcaacgg ctcgccgtac tgagcgttga agaatttgtg cggcaggatg
    34921 aaggcgagca cgcccttggg gttcatcagt tgcaggctgc gctcgacgaa caccacgtaa
    34981 atgtcgtagt tgcccttacc cgcagaggcg tagtgatcct cgaagtacgc cgcatcctcc
    35041 ggcgcgatac ccttcagcac ttggatgcgg atgtacggtg gattgcccac cacgagatcg
    35101 aagccgccag tccggcctgt cccggatttg gcagacgaat ccgcagcagc tttggattcc
    35161 tccggcgcaa ggtcgccatt acgcgcaccg atgaaccggt gaaagacctc ggcgaaatct
    35221 agctgccaga cgaaggtggg cttcttggga tcgttgaacc gttcggtcag gcgctccaag
    35281 gtttcgtcct gaacgctggc cttggtcttg gggcggcggg cggcggcgat ttgctcgcgc
    35341 accgcgccca tctccttcag cgcccggtca agctcggcca gccgggtcat ggccgcttca
    35401 tccgattcgt ccaccagcag gccgaagctg agtttggagc gggccagttc caccatcgcc
    35461 agttctgccg tggcatcgag aatgtcgaag cgcaggcggc gcttgtcggt gatgctgtgg
    35521 gcttcgtaga actggcgctt cgcgccggtc agcttgttga ggatgggcgg cagggttttg
    35581 tcctccgccg ccacgctggt gaggttgacc ggctcgccgt ggacgcgatc aatgagcgag
    35641 ttggcgacgc ggattttgaa atcaaggttc ggcagcgcgg gcagcttctt caacgcgtgc
    35701 cggaaatctt tggcggagca tttgtccaca tccacgtcca gcgggtgatc cacgatgagg
    35761 ctgagccaga gacggagttt acagatttcg acggcgcgtt cctgaatgtc cacgccgtag
    35821 atgacgcgct ggatgaagcg ggatttggtg atgaacaacc actccgggtc atcggccacg
    35881 gggtctttgc cgcgcaaccg tgtttcacag agccgggcca gattgacgag ttcgtagagc
    35941 aggcccaccg ggaacgcgcc cgagccgacc gccgggtcgc aggcccgcag gttgtcgagc
    36001 cgctccagca gcacgcgggc ttcgtccggg gtgaggcagc tttcgagcac ggccattgtc
    36061 tccggatcaa cgccatccga ggcgtcaagg gccagcagct tttgcaggcg gtcgggccag
    36121 tcctgggcgc gggcgggatc gggcggcgct tgctccagcc agatgcgcag gccgtcgcgg
    36181 caaaggtagt ggacaatcgg gcgcggggtg tagtggctgc cggtgtcgtg gcgcgaggtc
    36241 ttgccactgg tgtcggtctg ctcgatctgg agcaccagcg actcgaaaat cttgcccagc
    36301 atctccgggt caatggccac gtcctgattg agcggcgtgt cctcgcgtac cgtgaaattg
    36361 taggcttcca gcagttcgtc gaagacgtgc gcgaacacgc tgttgcagac tttgagcgaa
    36421 tgacggcggc gggcggattt atcacgctgc tccgcgccgt attcgtcggc aaacaagccg
    36481 ccgtttagga agggcagatc gaacccggcg atgtcggcca ttgaaccctc ggtggagagc
    36541 ttggtgaaca gcggtttgag gaatttttcg agatacgtcg cgctatcctt cgactcggca
    36601 aggtgttgcc ggaagtgccg gtagaggaaa ttgcggtcgc ggttcagcca gcccttgcgt
    36661 tggatgaagt aaaggaacag caggcggtca agcagggttt gggcttcgcg ttcgacgtgc
    36721 tcggagttcc accgcgactg gcggcgggcg atgtccttgc acaccagatc gaacacggag
    36781 cagaagtccc tgaagaattg ccgggtgacc ggctcgacat cgaaggccgc ttcgtggcgg
    36841 gcgcggatgg ccagcatgct gaggtcagtg acctcgctga catccagcaa cgccatgcgt
    36901 tcggcggcgg tgcggtagcg ctcgtccggg ccaagcttga agcgacggaa cacccggcgg
    36961 tcggcggtct tgacgttgac cagatgcgct tccgcgcccc gcgaatcacc cagcgtaccg
    37021 gcgcgggcga agacaaacag ggcgtcaggg aaggtttcca gcaacttgct ggcgagcaca
    37081 cgctgccgcc tgtagtccag ccggtcgcca tccagttcgg tgtaaataac gtggaacgcg
    37141 cctgcctccg cgagccggag cgggcggtac tgctgcgcga gatcgagtgc gatgccttcg
    37201 ccgaaggtat ccttggaaat cgggatcggg cgctcgactc gctggtagcc caacgtgttg
    37261 acgaacagcc gcagcaggtc gtcgcgggtt ttgatcgggg attcgagccg gtcgagaact
    37321 tgctgctgtt tccgggatga cgcggtgagt tcggcgggca tggctacacg agtgcttcag
    37381 tacagatgat tgccggttcg gtcgccagct tggcaacttc ggcgtgccgc tcggccagta
    37441 catccagctt gtgctcgaag gcgagggatt ccaaccgggc aagggcttca tcccgtgcgg
    37501 gcttttggtt gcgcaggccg cgcagcgcta attccacccg gccatccagc gggctgatgc
    37561 tccaaagact ccgaagccgt tccagacggg cgcactggtc ctttgtttcc ggtgtctggt
    37621 ccagcaactc ggcagcctgt ttataggcat cgcggatggt atccagcgcc caccgttgcg
    37681 aggcggtcaa ccggtggggt gtgccgcccg ccgcgaggta atcctgatac tgctggtcaa
    37741 acttggcttt gagcgcggtg accagggcgt tgtattgtgg cggaaggggc tggcgctttt
    37801 ccgtgggggg acaggtgatg gccgcgagcg aagaatgatt gtcctccgtc acgatgttgc
    37861 cctcggcatc ggccagccag agccgctgga aatctcccgc ctgcccgaaa aagaaaatga
    37921 ccgggcgcgg aggcgtggtc gggtccgccg ttgaccgctt ggcggtgcgg atggcgttgg
    37981 gcagtgacgt aatccgggcg aaaagctccg gctgctggcg cttgatccgg cgcagcagat
    38041 cttcagcctc ctgaacctcc agatcaacct gtcggtccat gtcgtcctcg atggcttcga
    38101 gccgcttgcc gtcgccctcg taaatggcgt acatcgcatc ggcattcagt tgttcatccg
    38161 gttcgaggat cgccgcatcc tcaccgatgg tctggtggat gtcctcaatg cgctcacgca
    38221 ggatggtgcg aatgcccaga tgccgttcca gttcgagttc gggcaggaag ttgaaggcaa
    38281 agatttccgt atgcggtgag ttgatacgat caatgcggcc aaagcgctgg atcaagcgca
    38341 ccgggttgaa gtgaaggtcg tagttcagga tcaacgcggc gtcttgcatg ttgagtcctt
    38401 ccgaaagcac gtccgtccca accaaaacgt gaatcggatt ctggaccggc gctttcactt
    38461 cattggcaat gggtgcgaac cggctgacca gggtttgaag gtcatcgcgg gaactatccg
    38521 cgtgctcgat ggtttccggc ggacggattt tggcagcccg caactgctcg gtgaggtact
    38581 gggcggtgtc aatgtactgg gtgaagatca gcagcttgtg ggtgcgcagg agcggctcat
    38641 cgtcgagcca ctgcaacaac cgctggagtt tggcgtcttt gtccggcgtg atcggctcga
    38701 cgtgcttggc catttccttc agcgtggcca tgtcggcggc cacatcccgg cgcagatcgt
    38761 ccagacggaa atcggcgggg tcgtacttct ggctcagctt ctccagctcg gcgcggagat
    38821 cggcgttttc ggaatcacct tcctcgacgc ctttgagcaa atcctccaca ccttcaccgg
    38881 cgataatcgc gccatcgtcc agaccattca ggaagatttt gtggcgctcg atcaggcggg
    38941 tcacggtctg gcggaaggct tccacgctgg attcgagccg cttgaacagc agcgtgcgca
    39001 tcaggccgtg taaacgttga cccgcccgca ccaggtcgtg gtacggggcg acatcctgct
    39061 tggccgggac gacgtaccga tgcaggccgt accgggcgta ggtcatgccg accttctttt
    39121 ttttgcccgg gttgggcggc tcgataaggt tgcgcagtgt tttgtagaag ccgccgtagg
    39181 ttttctcaat gctgtattca atcgttttca agttgcgctt cggaaagcgc acggatcgcc
    39241 cgtccacctt ggcgtcgggc caatgctcgc ggatgtgttt gcgcgtccgg cggatcagga
    39301 aatgttgcag cagcggcggc agcgggcgct cgtcgttttc gaccagcttg aagtacttgc
    39361 ccagcgagcg cggctcgacg cccagatcaa tccggtcgtc ggcgttgaac aggcggattt
    39421 gattcaggat gtcgcgggcg cggctgttgc gcggcgtggc ggtcaggagc accaccggca
    39481 aagccaagct gcggacaaac tgaaacagcc ggtcgtaccg gagcgagtcg ctgtggcgga
    39541 aattgtgtga ttcgtcgagc agcaccagtt gcttgttctg atacttctcg tcctcgaacg
    39601 cgaaattggg ctggctcaac atgcccaggc tcaggacttc cgcgccgagg tcgtaccggt
    39661 caacgaaccg gtcgcgccac atcttcacca gcggtgtggg gcagatgatg agcgcccgct
    39721 ggcgctcgcg gactttgagc catttgagca gcgcggcggc gacgaaggat ttgcccagtc
    39781 caaccacgtc cgcgatgaaa acaccgtggc ggagtcgcag caggcggatg ccgctcttga
    39841 ccgccgccca ctggaaatcg gcgagcgggg ggaagtcagc gggcatgggc acgtccggtt
    39901 cttccagccg gtctttcgtc aagtggtaga ggactttcag gtaaagctcg tacggcgtga
    39961 ccgattcatc cagcgcccac gatgactgga gttgggtcat caactcgggg tcgaacggtt
    40021 cggcctcgtc ccagagccgg ttgaaccacg tgagcaactg ctcgtgattg ccgttgccgt
    40081 ggacgacgac gttcaattcg gtgttgtcgc gcagcccggc cagactgagg ttggaacttc
    40141 ccaccaccgc gatcccccgc tcaaagcggt gggttggata gtcgaagatg taggctttag
    40201 cgtggaggcg ggcgcgagta taaaccctga ccttgagcct gcccgctgag atcagtcgca
    40261 ccagttgggt aatcagggct tgatcgtcat ccgtttgatc gagacgttcc aaccgttctc
    40321 ggattcgccg cgccccctcg gccacaagct cggcccgctg gcgggcattc agaaacgccc
    40381 cctcccgctg gtgggcgatg atggccgcgc gggaggcgtg gccctccgcc agttgctcga
    40441 tggtcgcccg gtcggacgtg ttgccgatca gaaggcggag ttcccgaacg tgggccagtt
    40501 gatcggcaat ggccttgaag cctgacagaa agaaatagcc aacggcaaaa tgagcacgca
    40561 cggattcaca gagcaacggg cgaaccgcat ccgcaaggag cagctcccgg ttgtcaatga
    40621 tgtcgtgcgg catagaccgg cagaccttcg caaaaaaatg aaagcgaaga aagccgaaat
    40681 gtttgcgact cccgcactct cccagagcct tttgttattg gtgcctgcgc ttcggcagat
    40741 gagggagaaa agattaaaat ccaaagccgc ttttggggtt cgggagatca ggcgcttacg
    40801 cgcatggacc ccgaagcctt tgccgcgctg ctacgcactt ggccccgtcg cgcaccgccc
    40861 gcgcccggca ggaaagcgaa taaccgttgc gccagccggc cgcaaggcgg tgctacgggg
    40921 tcttccggta gatgggcttg gggaccacga cggccggggc gcgctggccg cggatttcaa
    40981 tttcaagggg cgtatcgggg gcggcgcacg ccggcggcac ataccccatc ccaatcccta
    41041 tgcccagcga cgggctttgg gtgccactgg tcaccgtgcc caccttcgcc gcgcccggtc
    41101 ccgtggtcca aagggcataa tgcggccggg gcggggcgga tttgccggtc attttgaacg
    41161 ccaccagctt ccgggccggg ccgttggctt tctgcccggc cagcgccgtc cgcccgatga
    41221 agtcctcctt gtccagggcg acaaagaagc ccagacccgc ttccagcggg gtggtgtttt
    41281 catccagttc atggccgtaa agcgggtagc acatctcggt gcgcagcgtg tcccgcgcgc
    41341 ccaggccggc cggtttcagc ccatgcttga agcccgccgc cagcagttgg ttccaaatct
    41401 cctcgatcag ggttcccggc gcgatcacct cgaacccatc ctcgcccgtg tagccggtgc
    41461 gcgaaatcca tatccgggcg cccgcatgat taaagccggc cacctggttc ttcttgagcg
    41521 ccgtgacccg cgccacgctg gcgccgccgc tcgaggggcc gggaaagcaa tgctccagga
    41581 actccaccac ccgcgggcct tgcaccgcca gggcgccggt ggcgtccgag gcgttttcca
    41641 gcgtcaagtc atcgcgccgg ggaaaccggg cctgttgcgc ttccagccag gccacgtcag
    41701 cctcgatgcg cgaggcgttg atgatcaaca ggtattccca gggcgccagg tgataggcgt
    41761 aaaggtcatc tatcacgccg ccccgctcat tgcacagcag cgtgtattgg ccctgcccgg
    41821 ctgccagttt ccgcaggtcg ttggtgagcg tccaatttaa aaatgcctcc gcgccggggc
    41881 cgcgcacccg gatttccccc atgtgcgaga tgtcaaacag ccccccccct tgccgcacgg
    41941 cccggtgttc ctcgacgatg cccgtatatt ggactggcat ttcccagccg ccaaattcaa
    42001 tgagcttgcc gcccaatcgc tgatgcgcgg cgtaaagcgg tgttcgtttc atgcgctgag
    42061 catcctctca ttcccaaact gccagcaagc acaaatcaat cgtcccggcg ggcgggggcg
    42121 ggcggggttt gccgggataa cgccgccgtt tcaacctggc ttgaccgatc gctgggctgg
    42181 ctttgcccaa aggcccttga agctgtgaga cagggcgcca gttttgcgct tgagattgcc
    42241 gcttccggcc cgtaaatttg gcccccgaca gtgagcgcat acacggatca acgggataaa
    42301 acgctggtcg ccggagccgc cgggcgcaac gtcaaatccc acccacctct tattcgctta
    42361 tgaaccgcac ctttgtgatc attctcgtcc tgctcgtcct ggccttgggg ggcgggctgg
    42421 cctggcagta ttcccgaact accgcgctga aagcggagtt agccggcttg cgccaggccc
    42481 aacaggcggc gagcgaatcc agcgaagcgc tgcgcaccgc gcaggccgag ttggaatcgt
    42541 tgcgcctgca agccgaggaa ctcccccggc tccgccgcga cagccaggag ttgattcggc
    42601 tccgcaacga agtgcggcaa ctgcgcgact cgaaaggaga gtttcaacag aacctggacg
    42661 cggcgggcga ggagaatgaa cagttgcgcg ccatcgcgca gcaattgcgc caactggccg
    42721 ccgaaaacga acagctccgc acccagcttt accaggcgga aaatcccagc gcccccccgg
    42781 cgaccacgag cacgaagaac ctgtgtgcgc ggaacctgcg tcaactggat gccgcaatcc
    42841 agcaatgggc gctggagaat cagaaaaccg cgacggacac cgtcacttcc gaggacctca
    42901 tgccctacct gcccgccggg atgcctgcct gccccgccgg gggacaatac accttgagca
    42961 cggtgcgcgc cgggccgaag tgcagcattg acggccacgc cctcgtgcgt tgattggccg
    43021 gcgcggctgg agcttcgcgc cgaaagaagg gacgcgggct ttcccggctt gcggcccggg
    43081 cgcggggttt cgcgcgctcc cgcgggcacg ccgcgatgga atccatccca caaaacaatc
    43141 gccgacccgc ccgaggtttc ggacgggtcg gcgcgacagt gcgtttccgg acttaacggg
    43201 gcggactaag gcagcttgcc acacggctgc cacctgagcg cgcaaccggg aagatccgga
    43261 atctcagtgg ctatcgaacc cacttaatgg catccgcaac cacgacgtag ccggacggcg
    43321 cccagcagga gagggagacc ttgccggtgc ccgcattgag gttgaaggtg ccgagcaggt
    43381 tccatttgcc gccgttggct tgttgattca ttgtaacggt tgttgacccg ccactgtgat
    43441 aaacaatgta aggggccgca gtcgagcgat tggagcccgc actccaccag gcatatatct
    43501 tgtaggtgcc ggcgggcaga ttgctggccg accaggaggc cgggtcgctg accgcctggg
    43561 tcgagcgcca gtgatagttc gcgccgtatt tgtctcccgc agccgtgccg gtagcccagg
    43621 ccgaggacgc ggtgaagccc gcattgctgt tatccacgac caccggggcc ggcgggttaa
    43681 tcaggctcat gaaatacgtc cagttccaat agggacccgg atcggtgtgg gtgttgcagt
    43741 aggggttgat gcccaggttc gccgaagcat aggagcacca ggccgcattc gatttctccc
    43801 cgtgccccac aatgtggttc cggtccttgg caaagccaaa cttgtcggca aaatgccgat
    43861 gcaacagcgc cgaagcctga tacatcgcat ccgtgaacca ggccgggttg cccacaaacc
    43921 cttcgtgctc agttcccagt gagtgttgat tccaacacgt cgcgtgccag gcataatacg
    43981 cctcccttac cagttgggat atttcgcccg gcgcggcatc gccggcataa tccgtcttgc
    44041 cattcaccgt gtaatggcaa ctggcggaaa tatcgcagcg ccgcaggtag gaggtgccgg
    44101 ttaagtagta tccctccatg ctatggacga cggcaaactt gtggccgtag ccggaggtgt
    44161 accatttgga acagccggtg atcatgcgat ccaccgctgg gccgtagtcg gcggtggcca
    44221 gggcggccga agcaccgacg agtgatacgg aacttaacgt caggaactgt ttggtgttca
    44281 tacgaggagg tgttcttttt ctacatgagt acgtagctct tggttttggg gtttttctgc
    44341 cgacactgcg ataagtgctc ggtgtgaata ccagcattca caagcgcgat ttcagcacca
    44401 agccgccgga tgcgcaagca gaaaattcaa aaaaatttcg ggcacccgaa gcccctccca
    44461 accaggccgg aaacccgatt gccgggggga gtaaaaccgc ctgggcttgg ttacggcggc
    44521 ccctcccgtc ggggaatccc cacgcgcggc ggcggggctt tgacgaagcg ggcttcccgc
    44581 aggtaaatcg gctccagctt ctcgcccgcg atgaagtcgc gccgcccttg agccagttgc
    44641 ccgatggccg cggcgcgcgg gaaaagcatg cggctgcccg gaaaccacag ggccacttcc
    44701 gggccgagga gggtcacgcc agcttgttgc cgggccagga cttccgcccc gggaaccagc
    44761 cgcagggggc ccacctcccg ccgcgccgtt gccgtcagtt catagtcagc caggtagaac
    44821 tccccgcgct gggcatcaat caccaccccg atgcgcccgc ggaggccgtc cgcctgggcc
    44881 tgggcggcaa ggccctcggc gctgctggcg cccagcaact gcaccgggcg cgccagttgc
    44941 cagccttgcg ccagggaaat ggccaggcga atgcccacgt aggagccagg cccgagccca
    45001 accgccaggc attcgatctg ctcgcgttcg aggcgcgcct gccgcaaggc ctcttcgatc
    45061 agcccaaacg ccctggtggc gcgggttccc gtctcgctgg cttcgcccaa aaccgccgcc
    45121 ccggcgcccg ccccctccgc caacaccgcc acgctgcgct gcgcggaaga aaactcaaga
    45181 gccaatatct tcatacgtaa tgcgccgcgc cgtctcgccc agtgtgtgaa tctggatcca
    45241 gcggaaccga tccgccggga ggaaaggcgc cgccggctgg tcttgcgcct cgccaaacca
    45301 gcgctcggcc cattcaatca ccgtgacgcc cgccggggtg agataatcct caagcccggc
    45361 ggcgaggatc tgcgccgggg tctcgagccg gtataagtcc acgtggaaca acgtcagccg
    45421 cccgccggaa tagatgttga ccagcgcaaa cgtgggcgag tgcacccgcg ccgcaatccc
    45481 cagcccgcgg gccagcccct tgacgaattg cgttttgccc gcgcccaggt cgccggacag
    45541 gccgatgacc catccgctgg gaacggcgcg gccccattgt tcgccgagcg caagggtctc
    45601 cgcggggcta tgcgaaatga gcgtagccat gatccgtcag ggtccgcgtg ccatgtttat
    45661 cccgcagggc gatgccttcc ccggctgtaa tcttgccgat gcaggtgagc ggcaggccgg
    45721 ggaactgctt ccgccacgcg tcgaggagcg gcaccgcgtc gcggctggca acggtgaaca
    45781 gcagctcgaa atcctcgcca tccgtgagcg ccgccagcag cgccggtttg gctccggagc
    45841 cggcccgggc cgccagccgc gccgcgcggc tgatgggaat cgcagcgccc agcagctccg
    45901 cgcccgtccg gctcgcgctg agaagatgcc gcaaatcact ggccagcccg tcgctgatat
    45961 ccagcatcgc gtgggggggg aaatggcgcg ccagccaccg ggcctccgcc aggcgcggct
    46021 cgaagtccag gtgcttcccg gccagcgagc cgcccagttc gccggtgacg aagatggcat
    46081 cgcccgcttc cgcgcccgag cgcaacacgc cctgcccgcg cggcacccac ccgagcagcg
    46141 ccaccgaaat gaacacgccg ccggggctgg ttgtggtttc ccctccgacg atggccacct
    46201 cgcaccgccg cgccagggcg ttcagccccg tgtaaaccgc ctccacccag tccgcctgaa
    46261 acccgcgcgg caggccgacc gtcaccaggg cggcggaggg cgttccggcc atcgccgcaa
    46321 tgtcgctcag gcaccgggcc agcgccttgt gcccgatttt ctccggggga gcgtcggcca
    46381 ggaaatgcac cccctccacc accgcatccg ttttgaacaa cagcagccga tccggcccgc
    46441 cgggctccag caccgcgcaa tcgtcgcccg cgcccaccac caccccggga ttggtcggca
    46501 gggcgcgcgt gaggcggcgg atcagatcaa actcattcat gcgcctccat aatgctgcag
    46561 caccagcagg gccgcaaagt tcagcgcgtt aaacagcagg tgggcggtga tgggcgccag
    46621 caggttgccg gtccgctcat acagctccgt cagcagcagc gccagcacca gcagcggcac
    46681 aaaggtgacc aggttcaaat gcaccagcgc aaacagcagc gccgtgcccc accacgccag
    46741 ccgcggatac cccgcctgct tgatcaccgg atacagaatg ccgcgaaaca tcgtttcctc
    46801 cgccaccggg gccagcaaaa ccgccgccgc ccccagcgaa agccgcccgc cccacgtcag
    46861 cgcctcccgc aaggcagtga ccggcagttg ttctttgggc gtcaagccca ggtgcggcag
    46921 atgcgtgagg accagcgccg agaccttctg caatccccac cccatcggca gaaaaagcag
    46981 cgcccccagc gcccccatca gcgcggcccg acgccagcga tgcgccagcc cgaacgccgc
    47041 cgtccagtgg gtctgctgct cccgcagaaa acgtccgatc aaaatcaaac cggcgccgtg
    47101 aaagctcaac gccgccatca gcatccggct cagcgaagca tcgcccgcgc ccgcgcccga
    47161 aagctgctgc gcccacgccc ccagcagcaa cccgctgtaa gcgcaaacca ccaggcccgc
    47221 cagccgccgc gaaatcccct ccggcggcca ggcccgggcg atgagcccca gggccgccgc
    47281 caggcaaacc agcgcccccg ccgccagcgg gaaaaacagc ttcgcgttgc cccgggcccc
    47341 ggcggccgcg taagcctgca cgctaatcag caccgaaccg gcgtacgcgc aaagaatcac
    47401 gctcagcacc aggcggacaa ttgcctccac cggccacggc ttaacggtta acataccgcg
    47461 acactatcgc acttgcgccc ggctgacgac gcaaaaagct gccggacccg gccggggcgc
    47521 gcccctcaca gaagagcgtc gcccgccaac tttcgggttg gaataatgcc cgcattgaaa
    47581 caggtgcgcc atagcttgct attccgccaa gggcaatacc ttgaactcat cccccatacg
    47641 gaaacgattc agttggaaaa accaatcatc caccctcgtt ccctcggagc ccaggcaacc
    47701 ctgcccgccc aagccaatag caccggcaaa acccttattg agcgaaatca gcggcatcgc
    47761 taccgaaccc caaacgactt tcgcttgtaa aatcaatagc ttacgcacgc gaagctgagt
    47821 aaaagggagg caaaatctga atccagacgc cttcggcgca ccataaacca gccctgatct
    47881 taccctgcct tcaggccgtg tacaaggggt gtacaaggga tgcccatcgg gtgtaccagc
    47941 taaaatccca tggactcccg gtgggcatcc cttgtacacc ccttgtacac ggcgcgaggg
    48001 cagggtattt tcaaggtatc tggatagtgg atggggatgc ctggaaaggc caaaacgatg
    48061 agctggaata ggaggacgag tagtaatttg ctgaaaaaca gttagttgtg aatctaagcc
    48121 ggaacgattc agttgaaaaa gtaagcaccg tttggcggac ttgggag
//
LOCUS       DAOPQG010000009        47390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_981_length_47390_cov_14.283258, whole genome shotgun sequence.
ACCESSION   DAOPQG010000009 DAOPQG010000000
VERSION     DAOPQG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 47390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47390
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_981_length_47390_cov_14.283258"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKI07_01885"
     CDS             complement(<1..257)
                     /locus_tag="PKI07_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49830.1"
                     /translation="MNTLFNSHTLGRAFPWRRGWISLISLLLLLPGAAWAWWGDAYKT
                     PTGLSGGWATHTDCGTYWYTYGRNTSCTAFNRWAQFIPGFV"
     gene            complement(264..2027)
                     /locus_tag="PKI07_01890"
     CDS             complement(264..2027)
                     /locus_tag="PKI07_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC49831.1"
                     /translation="MATTELLPAGDTNVIATYDGGRVSAGDVAEAREEPRFVADAEQN
                     APTNAVSLDEKLARHLAAVRILVSEARRRGLDRPVDPEAAKAGPQPQHQPYAQAGPGW
                     ALEQKLIEQRVLAQALEKEVRRSVTISEQEIDEQYRTNRFNMIGFEAVEAGRIGISAQ
                     KHGEGALERAKQALGLIRNGQDFVAVARQYSDLDLKLAQTAPYPATFWGKENGLVLGQ
                     LGEGAVSEPVPVEDGYELLRIVRLHLTGNPSPEEVKAQLRLMLPEITAQERLQDMAQA
                     AASAFPFVTSSTNASSPAEVHPPATPPSAAGQVLLRCGQFALTRGEVRGLALQRGAQT
                     VDDPQMVEILRQENRDSIQMGELARSMGYRQWPEVRRALRYELDKQLAEKARRFLLPE
                     LLAELTFPEEQVRMIYDTRFTATFPPQIEYDALVVPVRAPAEATAEEREAVRTNAFGR
                     AEEILRRVQDGARLEDIVSTDAALQWLPGQSRVVEENSALAPLVAGLEAGAVAVVPYE
                     DFGGFCVLRVGKSELRRKMPYEIARNYIIHDLRGEAVRDFHQHFETILLNRHHFFFGT
                     GAAGQTRVSAPADPARVNNQP"
     gene            complement(2988..3710)
                     /locus_tag="PKI07_01895"
     CDS             complement(2988..3710)
                     /locus_tag="PKI07_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOC49832.1"
                     /translation="MTRPAPNPAARRAQALRLAYQRMRARFGHLQWWPGETPFEVCVG
                     AILTQNTAWSNVERAIANLKAARVLTPRKLYALPERNLAALIRPAGYFNVKARRLRAF
                     LQTLVEDFGGRLDRLLAGDTATVRQRLLAIHGIGPETADSMLLYAGAHPRFVIDAYTR
                     RIFQRHGWCRGDASYDDLQTLCESALSQKPLPARLDYWQDYHAQLVITGKTYCRPRQP
                     RCAECPLKPLLPNRPQSKTIGH"
     gene            complement(3707..4954)
                     /locus_tag="PKI07_01900"
     CDS             complement(3707..4954)
                     /locus_tag="PKI07_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOC49833.1"
                     /translation="MNPPHVVVAGGGAAGCFAAITCAEAAPATRVTVLEKSPLFLAKV
                     RISGGGRCNVTHACFEPRDLSRHYPRGGAALRGPFTRFQPRDTRAWFERRGVKLKIEP
                     DGRMFPAGDSSRAILECLTRSARAAGVTLRPRCEVTALARRPAGGFTLALAGGERLDC
                     ERLMLATGGCRTAADGSLPLALGHSLEPPVPSLFAFRLATPWGRELAGVAVADAELSL
                     PGPGLRERGPLLFTHTGLSGPAILRLSAWGARALHAARYQFPLRVNWLPHLGPAALAA
                     QLDHRRAAEPARRIANSPLSPLPARLWQRLVQAAGIPPETRWAGLPRAARHQLIEHLR
                     ATELQVNGRSPNQDEFVTCGGVRLREVNFHTMESRLCPGLYFGGELLDIDGLTGGFNL
                     QAAWTTGWIAGRAMAGLTGQTPP"
     gene            complement(4951..5757)
                     /locus_tag="PKI07_01905"
     CDS             complement(4951..5757)
                     /locus_tag="PKI07_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOC49834.1"
                     /translation="MKTQAPNATMAVYLDLENIARGALDARYPTFSIQKVLERLLLKG
                     HIVVKKAYCDFDRYKTFKRDLHEAAFELIEIPHVRQSGKNSADIRMVVDALDLCYTKG
                     HVDTFVIISGDSDFSPLVSKLRENAKTVIGVGVKNSTADLFISNCDEFIYYDDLVRAA
                     PPRARRQAAASAPAAKPAAETNAGPSREKAFELVEETLEAISQEREADEPIWGSMIKQ
                     AIKRRNPGFNERAYGFRSFNDLLQKAQERGLLQLQADEKSGGYTVRALPE"
     gene            5923..8748
                     /gene="polA"
                     /locus_tag="PKI07_01910"
     CDS             5923..8748
                     /gene="polA"
                     /locus_tag="PKI07_01910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOC49835.1"
                     /translation="MAKRLFLLDGMALVYRAHFAFIARPVLTSQGVNTSALYGFTQTL
                     LNILGGWEPTHLGVAFDTAAPTHRHREFAAYKATRQEMPEDLSAALPHVRRMLEAFNI
                     PALALDGFEADDLIGTLVRRAEREGFQSYMVTSDKDFGQLVSAHNFIFKPARGGDGAE
                     ILGLAEIQARWGVSRAEQVVEVLALMGDASDNIPGVPGIGEKTAVKLIAQYGTLENLL
                     AHVGELTGRVRQALEAHPEQARQSRRLATIRCDAPLELELDALRVRPRNEEKLRELLA
                     EFEFNALGRRLFGEEFRAGRARGSVPAGAGGARGRAGEQLVLLGEPEAAAAKPKPGAA
                     ARPGLKTLAEVPHQYHLAATAAERAQLIRKLQGLDSFCLDIETTSLDPKAARLVGLAF
                     SFAAHSGYYVPLPPAPRAAPVLEEFRPVLESERIEKVGHNLKFDLSVLKWQGISVGGK
                     LFDTLIAHSLLEPEMRHGMDHLAEVYLDYTPIPIGRLLGDSGAARAGLADVPVAQVAE
                     YAAEDADVTWQLRAVLEPLLKARGQERVFYEVEAPLIPVLVDMEYEGIKVDAAALAEF
                     AAQLAGEMGAAEKAIYRLAGTPFNLNSPRQLGQILFDVLKLGAAPRKTRTGQYATDEQ
                     TLQALAADHEIVRRLLEYRAAAKLKSTYADALPEAIWPGTGRVHTTYHQVMTATGRLS
                     SQNPNVQNIPIRTERGQEIRKAFVPRGPEYRLLAADYSQIELRILAALSRETVMLEAF
                     RSGADIHTATAARVFGIPPGEVTPEMRRKAKMVNFGIAYGISAFGLSQRLGISRAEAG
                     TIIAQYFQQFPGIRRYRDETIARARERGYVETVTGRRRYLRDIRSANDTARAAAERNA
                     INAPIQGSAADMIKLAMVAIHRELVRRGLRSRMLLQVHDELVFDLYLPEREEVRRLVG
                     EIMTTALPLEVAMAVEMGEGENWLAAH"
     gene            8852..10690
                     /locus_tag="PKI07_01915"
     CDS             8852..10690
                     /locus_tag="PKI07_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC49836.1"
                     /translation="MSRHPKTWAQPDPACRLPDQAPGARAAVWTGGGRARRGWGGGGG
                     VAAVVLIGLLLSGCTARHYRRSADKESYAIIQQMERRVFGHTNEFSIQTRYSDRDPKT
                     ISPAEVLDDRAVSNRFFVNLDQALQLAVENSREYQTQKEQLYLAALALTGARYEFSPQ
                     FFAQATPQIAGSPDGADVGSVASKVGVNQLLKTGGSLTMALANDLVRYFGGQPAATAR
                     QSVINVLSVNLTQPLLRGFGINSPAVEALTQAERDVVYAVRTYSLYQQEFAVGTVSAY
                     FGLLTQKDIVRNNYRNYTNRVETTRYLQARAVDRERLSNADDARTAELKARTDYIDSL
                     ASYLSALDAFKLRLGIPLAAQLFLDDRDLEELIGAGLIPVDLDRRAAFAICVQSQMEV
                     LNAIDRFEDSKRKVRVAADQLRADVNLIGSASLASEPPDDYTNFDPGKVQYSAGVRLN
                     LPLDRLRERNQYRRSLVAFEAQLRALSLTLDNFRDRLDRGLRTLEQARLNHVSAAESL
                     KVAQRRVENNLMLMEAGRATIRDVREAQDGLIQAQNSLATIYAQYLTARLTLLLNVGL
                     IDTRPERFWLQDPLRERLTPEQRGPSPLRMPDDQVLPPENFLEPTS"
     gene            10705..12675
                     /locus_tag="PKI07_01920"
     CDS             10705..12675
                     /locus_tag="PKI07_01920"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC49837.1"
                     /translation="MDKLPPVLRRRPVLFGAAGLVLLVLVILWLRPARPAVEPTAYYE
                     VKRGDFTVSVVEGGTLSAVSEYSIRSEVEGTARIISIVPEGSYVKKGDLLVELDSASA
                     QDQVNQQQINYEKAKFSVEQALAQLEIQKSATNSEYLAAELKLKLARIDLEKYEQGQR
                     LVDLVEASNKLVQAQAQLMVNMDTYRNSTNLAAKGYETKQKVDSDRLAVLNSANALIV
                     ASNSIWMLRQFDEVKQAETYRAAVLQAEQELDRVVSQNVRKMAQYSADLITQSNTLAL
                     SEQKLERDKKNLAATIIRAPQDGLVVYPVSDSHFSSESLIEGGAIVRNRQELIKLPDL
                     SRMKVVIKVHESHVNMIRPGLPAFVMLDSMPDERFRGVVEKVAPLPDTQSRWGNPNLK
                     VYNTEIYLTDTLPNVKPGVSAKAEIIVTNIAGAISVPIQAIATYQGQPAAYLVHGHRS
                     EPRPVEVGMFNTKFIEITKGLKPGDRILLSPPFEAREKDLEGSVLAEGEKAGAATNAP
                     PRTTPPAPAPGLSSGEPAAGGEERAADSRRARGNFNPEEMLKQFDKNGDGKLDDDERE
                     AMRAAMAARLGGGSGASGTNAPPRLSPEEVLRRFDKNGNGKLDEDELAAMRESLGRGR
                     GGLGKGPGASGAGNAGGDAAAPRRQLPAPAKP"
     gene            12672..13466
                     /locus_tag="PKI07_01925"
     CDS             12672..13466
                     /locus_tag="PKI07_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC49838.1"
                     /translation="MSSVPPKPSSARRPSGRAGAAAGTAFGESIIRLEALQKTYVLGE
                     ENIVRALDGVDLTIHAGSFVAIMGPSGSGKSTMLNILGCLDRPTAGRYFLGTEDVSRM
                     SDDALSEVRGRRIGFIFQSYNLIAQLTVLENIQVPLFYRGQDIKTSRDRCVELAKLVG
                     LGDRLTHRPSQLSGGQQQRVAIARSLVNDPLMILADEPTGNLDSKTGTEVLELISRLN
                     QDGKTIVLVTHDDKVAERAQRVVRMKDGRIDRDEQRAPNATAAPAR"
     gene            13471..14763
                     /locus_tag="PKI07_01930"
     CDS             13471..14763
                     /locus_tag="PKI07_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC49839.1"
                     /translation="MIPYQFLITVRLGFKSLKLHKLRSALTMLGIIFGVCSVIAMLAI
                     GEGASYEAQEAIKRLGSNNILIRSLKPPEQARQQGAGSRRGFQIKYGLTYDDAGRLLG
                     TIPGVSRVLPMRIIRENVRFQRTEIPCQVIGTLPFYPEVAGATLVRGRFLAYYDEEHM
                     ENVCVITTGLARQLFPYQDPLNHAIKIATFYYRVIGIVQERSQPEQRSQAGKMEGQPL
                     DNNVYIPLNTSRTRFGEILFRRSAGSFEAEEVQLHQVTVQMRDLASVETADPQIRTLL
                     GRFHEDNDYEIIVPLQLLRQAEQTKRIFNIVLGSIAAISLLVGGIGIMNIMLATVTER
                     TREIGIRRALGAKKADITMQFLVETVVLAVGGGLVGVAVGIITPVLVSHLTTMKTIVT
                     LWSVLLAFGISGAVGVIFGLYPAKAAADLDPIEALRHE"
     gene            15216..16145
                     /locus_tag="PKI07_01935"
     CDS             15216..16145
                     /locus_tag="PKI07_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOC49840.1"
                     /translation="MARDARIYVAGHRGLVGSAIWRELQRRGYTHLIGRTRAELDLLD
                     GAAVNRFYAQEKPEYVVDAAAKVGGILANDRHPAEFIYQNLQIQNHLIHGAYVAGVRK
                     LLFLGSSCIYPRLSVQPMKEEYLLTGPLEPTNEWYAVAKIAGIKMCQAYRRQYGCDFI
                     SAMPTNMYGPNDNYDPQNSHVLPALIRRFHEARQANAPTVTCWGTGSPFREFLYADDL
                     ARACIFLLEHYSEEQFINVGSGTEVSVRELTETVGRVIGYAGRIEWDASKPDGMPRKL
                     MDSSRLFALGWKPQVDLETGIRLAYEDYRKRFG"
     gene            16166..17365
                     /gene="lhgO"
                     /locus_tag="PKI07_01940"
     CDS             16166..17365
                     /gene="lhgO"
                     /locus_tag="PKI07_01940"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOC49841.1"
                     /translation="MASRRVVIIGGGIVGLAAAHKLAARWPAARILLLEKEGRVCAHQ
                     SGHNSGVLHSGLYYQPGSLKARLAVAGIREMVAFCQDHAVPHEVCGKLVVAADDAEVP
                     RLRRLQERGAQNGLEGLRWLNREQMREVEPHVGGVAALQVPQEGIVDYPKVCEALQRE
                     IEARGGSVVVGARVTALRLRNGEWRIESPKGDWAADFVINCAGLHCDRVSQLAGEKRE
                     VRIVPFRGEYYALKPEREHLVRNLIYPVPDPQFPFLGVHFTRLIHGGREAGPNAVLAL
                     AREGYRKTDVRLADVWDALTFSGLWRFLARHKRMSWDELRRSFSKRLFCASLQRLVPE
                     IQEADLAPGGAGVRAQAMAPDGTLVQDFCLLQRPQALHVLNAPSPAGTAALAIGSEIA
                     NLVASES"
     gene            17396..18328
                     /locus_tag="PKI07_01945"
     CDS             17396..18328
                     /locus_tag="PKI07_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC49842.1"
                     /translation="MKILITGGAGYLGSVLTPTLLGLGHHVTVVDNFLFRQNSLADCC
                     PYEGFQIVRGDCRDEALMKKLVGGADVIIPLAALVGAPLCDRDPVAARTTNFEAVAML
                     CRLASAAQRILMPVTNSGYGVGEKDKFCTEETPLRPISLYGTTKVQAEEVILQRENSI
                     SFRLATVFGVSPRMRIDLLVNDFVHRAVNDRAVVIFEGHFKRNYIHVRDVARVFAHAL
                     NRFEEMKGKPYNVGLDEANLSKLELCAVIQKHLPKFVFLEAPIGEDPDKRDYIVSNAR
                     LAATGFTSEWGLDRGIRELIKGFTILRNSVYANV"
     gene            18337..19080
                     /locus_tag="PKI07_01950"
     CDS             18337..19080
                     /locus_tag="PKI07_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOC49843.1"
                     /translation="MTRELSQTVAVVLAGGLGTRVAHLLPGVPKPMAPVSGKPFLEWV
                     VRWLAQQGIRKVILSTGHLAGVVAAHFQAQPVPGVLTRCLAEPQPLGTAGGFLHAARL
                     SAETPPAWLVVNGDSLVLASLAAAARELHRHPAVAGVVVGRAVPDAARYGALTLGPGA
                     ELRGFAEKQPGPGVINAGIYLLRDTLVRQLPGQWPLSFEQEVFPRLLAQGCALKVCVV
                     EAPFLDIGTPESLRQAEAFVQQNRAQFGD"
     gene            19088..19636
                     /locus_tag="PKI07_01955"
     CDS             19088..19636
                     /locus_tag="PKI07_01955"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOC49844.1"
                     /translation="MMKKSGSNREAVFTSPWFQVLAESTPGGGPPHYVIQGLDFVVIV
                     ALTRQDQLLLVRQFRRAAGAITLELPAGHIDPGETPEQAARRELAEETGYEADHFELL
                     ASLSPSVARFTNRMWCFLATGARPATSPQTEREAGVDLILYDRGVQELIGEKDFYSAP
                     NYAALLVAVLRGKLKPRPDLHR"
     gene            complement(19758..21755)
                     /locus_tag="PKI07_01960"
     CDS             complement(19758..21755)
                     /locus_tag="PKI07_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOC49845.1"
                     /translation="MLRPVFGLFVIWLVAFQLLRGLLIITTWSHRGGAPMPLIGLSFW
                     HGLRFDVATAVWLTLPFALWRILRAAPGRRERGLAFAIYALLALAGLFALIAEVEFYK
                     EFQMRLGPLAYEYFSTQAEHNAIVLGMIWHGYPVVRWTLVCLALWAVFLWATRRLFQP
                     APGQVGWLPRLLVVAALAGVAVVGIRGGLQHTPLRWGNAYFSPSTYVNQMTLNGLWSL
                     LDSLGSHSSATQKSTRQWQRRMPLDQAIQKVRESILLPGEKLVDAGKYPLLRVSPPNE
                     AVVRRPRNVVVVIMESFTARFNDALGASLGATPCFAALAREGILFDRAFSISTHTAQG
                     VFATLCSFPNLPDNDRPAKGPVGEQPFRSLPRILQERGFATLFLYNGLFSWDNKEGFY
                     RHQGVQRFIGRNDFKNPTFVDPDWGPCDFDVFNRAVEEFSALSAGKKRFLGIILTLSN
                     HAPFDLPPVAGLEPITDGGEQNKRLNGVHYADWALGQFMASARKQEWFADTLFVFVGD
                     HGFGIPPNLTETSLLHMHVPLLFYGPELFGGQHEVRHQVASQLDILPTVVGILGLDTP
                     HQAFGRDLFRLPPDDPGQAYVKRVGEPILGWIQGDEILTASLDAPAHFYRLGLGFPPS
                     ASADLASAQPERATAFSRELDAFVVAGLHTLNRRLASAPAR"
     gene            complement(21972..22700)
                     /locus_tag="PKI07_01965"
     CDS             complement(21972..22700)
                     /locus_tag="PKI07_01965"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOC49846.1"
                     /translation="MATMVQERDPAETRPPEPAARPGALLVKALLFLAIAIAGIVAYR
                     FTPLKEWLQPAGAAADWVRQTGFRGVAAFFLVMALLIVVGVPRLLFCPLAGALFGFWG
                     GMAMSVLGTMASYYAAFLVIRGRRHNTNQAPPLHPKLAFLADQPGLAAVIVSRMLPVP
                     GMVATVALALSNVRTGVYLAGSLVGLIPEAVPLVLLGAGMLHSSFEKFFKLALAAFVC
                     ILAAWVAIHCFLKHFKPGRPAHQP"
     gene            complement(22704..23450)
                     /locus_tag="PKI07_01970"
     CDS             complement(22704..23450)
                     /locus_tag="PKI07_01970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC49847.1"
                     /translation="MTLPSPSLSVVIPVFNEEDNLPALLGELTAVLRSLQCPFEILCV
                     DDASTDGSWAVLRQLRARHPELRILRHRVNSGESAAQASGFARARGEILITMDADLQN
                     DPHDIPAMLAALQPGVAAVAGVRRQRLDDATRRISSRIANGFRNWVTGDRIADAGCTF
                     RALRRAAVRELPVFNGLHRFIPTLLRRQGYQVEEILVNHRPRTRGQSKYGVGNRVWRG
                     LRDCFGIRWYSARAVRGDRLLPEEEAPPAG"
     gene            complement(23474..24592)
                     /locus_tag="PKI07_01975"
     CDS             complement(23474..24592)
                     /locus_tag="PKI07_01975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC49848.1"
                     /translation="MHSTRTVKFVDLARLHAPIAAQVDAAALRVLREQRYIQGPDVRE
                     FEKEMAASLDGAPVCSVSCATSGLLVALLGLGIGPGDEVITTPLTAIAAAEAISLAGA
                     KVVFADLEPEGFCLDPEDVRRKITPRTRAIIPVHIYGLLADLDALQEIARAHGLKLIE
                     DCAQAQGARYRGRRAGTLGDAGVFSFFPSKNLGGFGDGGAVTARDPEVFRRMRMLANH
                     GRESKYDHELVGLNSRLDTLQAALLRVCLPSLEGWNAARRAAAKVYDQLLGGIPQLQT
                     PRARARTEPVYHLYVVRAPDREALADHLRGRGIETGLHYPQPLHETGAYRHLGYRRGD
                     LPRAEAACRSVLSLPMHPCLTREEIETVSRAVADYFQP"
     gene            25203..27365
                     /locus_tag="PKI07_01980"
     CDS             25203..27365
                     /locus_tag="PKI07_01980"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOC49849.1"
                     /translation="MACANLAFFLSPQGRNSWPYQPEQPVSLPEDFFGVCVASSEDPA
                     CDDYILARLRELGVKCVRVDYGYDSPGGFADRLVGRLLSEGYRVALHLVAPRRDMEAL
                     DTPDGAARWREFVRDAFARFGGGVELFEIGSTVNRRRWSGFTVPRFLRAWEIAWAEAR
                     ERGLVLAGPNVTDFEPFYNVAILQALRQRGCRPGIHTNNLFVERATEPEAFDPKILGP
                     GWAARLKFNTVRKAALLGAIGRWAGVPRTYSMHVSWSLRRIRRLLVDVEEQQADYVAR
                     YGCLAAASGGLQRVYWGPLIGQREGLIDDGTTFFPEVFHVTFYGRANGKVGDYRIRPA
                     FRAFQTVVRFLAGTTFTRRISARGSLEILEFQSAAHTLHAVWCRNGDCAVAADCYAAE
                     ALGAAEAFDRTGALLAAAPVLFTQRPTYLRWKAAAAIRLAPAPGSMADLRLAARGEAE
                     YVPIRQPDWRGLALARLAGDKIDPAALLPGRLAPMDSAAARVLRNKRNRVWSVAAPWD
                     ARRQIVVKHFRPATGLRGWRQRFKAGKARRSWNGAQELLRRGIPTPEPVAWLEHPARP
                     HAHESYYLCAAFAGGTSARQAFYAVNGGAAEFLGEPVAGLFRQIAELLARMHGRGVFY
                     RDLSAGNLLLRRGADGNLEFALIDTGRARIGVKGLGIRPRLADLMRLCHPLAWRGREQ
                     FLRAYFLAANIRFAGWMRLAFHYYDWKHRLKKWLRPWR"
     gene            complement(27433..28560)
                     /locus_tag="PKI07_01985"
     CDS             complement(27433..28560)
                     /locus_tag="PKI07_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC49850.1"
                     /translation="MRIGIIKSNFFPSGGGSERYTHGLVTQLQARHHAVHVLAARWDE
                     TAAAAGVTLQRVPALRWPAFARVLSFALNSRRALPTAGCDLILSIERTLRQDISRAGG
                     GCHREWLRQRQRYRPGGARALAGLNPLHRALLWIEKRTFSPANTRVIIANSHRGKEEI
                     IRHYGFPAERIRVIHNGTDNARFRPPPAPPARPQTVLLLAGSGFERKGLEFALRALAR
                     LPQTVRLEVAGKGRTAPYQRLAARLGVADRLRFLGSANRMEDVYAGADILVHPAIYEP
                     FSNACLEAMACGLPVVSSRVNGAAEIIRPGLNGSVVEDPADSAALAAAITPFLDPAAR
                     ARAGAAARQTAEALPMSLNVEKTLAVIEELRANPLPPASPP"
     gene            28945..30501
                     /locus_tag="PKI07_01990"
     CDS             28945..30501
                     /locus_tag="PKI07_01990"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOC49851.1"
                     /translation="MKPHVEQTQNAAAGQVCRITAGRWRWEVRAAHATPAMQSAWAAP
                     EQLVAPPAERVVRNPPKRTTQVFRARLPGSGGRVFIKRYLAEAWQSCKSWFRLSKAVR
                     SFRRGLELQRLGVPTAPPVAAGEYRVAGWLRDAWLITEEIPDARTWLECDLAAPRGAH
                     RVPMLRALARCFAQLHEAKLSHSDANRNNLLIQLAPGRRPRLYLIDLDALVRRAWFPR
                     WRVVKDLRRLHARGPATRREKLWFIAEYCRARKGALEPREWVRRVGRSLAMPLTVRRV
                     GPVRWVVRPVLQADALQALLQDPEARLRDAALRLKNSRNVTLVRIPQPAGPGWVLRRL
                     NYGRLRHRLRDCFRPSRARRAFRKGLWLEQAGVAAARMLAVGERRRLRWPVCAYLLMT
                     EIPGAITLAELLARRPPQLREALRRLAQELALLHDAGFSHRDLKATNVLFDGQLQPHL
                     IDLDGVRRVRWGATARAEADLERLARETLVHPAVSTRQAAFFLKTYCRRRGEADWRGW
                     WRAIQKRLSG"
     gene            30608..31840
                     /locus_tag="PKI07_01995"
     CDS             30608..31840
                     /locus_tag="PKI07_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC49852.1"
                     /translation="MTSRERVLTALNHQEPDRVPIDLSGHRSSGIAAMAYARLRRYLG
                     LPPRPIRVYDPVQQLAIVDEDVLQRFQVDTLELGRAFALEDKYWVDWTLPDGTPCCMP
                     AWAKPDRLNGEWVLRSSVTGKVIARMPEGVWYFEQASFPFLEREDLDRLEAEFSECMW
                     SGIASPPGPIAAGPDGMAVLAAGARALRQQTDRAIIGLFGGNLLELGQWFYRNDNFFM
                     LLAGEPERAHRFLDKLTEIHLRNLEGFLAAVGAHIDIILFGDDLGMQSGPQVSPAMYR
                     EYFQPRERAMWQMVKQRAPHLKIMLHCCGGVRELLPGLIDAGLDAINPVQITCRGMEP
                     SALKRDFGERLTFWGGGCDTRDVLIQGTPEQIREHVRQQIRILRPGGGFVFQQVHNIL
                     ADVPPANIVAMYEAVIGA"
     gene            31926..32318
                     /gene="hisI"
                     /locus_tag="PKI07_02000"
     CDS             31926..32318
                     /gene="hisI"
                     /locus_tag="PKI07_02000"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOC49853.1"
                     /translation="MSFYDQLKFDAHGLIPAIIQEQSSGRVLMMAWMNRASLERTLAT
                     GKTHFWSRSRQKYWMKGESSGHTQTVKSVSFDCDGDTLLIQVEQIGAACHEGYQSCFF
                     RTVQSDGVTSRVTEPRLQTPEQIYGAKP"
     gene            32318..32953
                     /locus_tag="PKI07_02005"
     CDS             32318..32953
                     /locus_tag="PKI07_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49854.1"
                     /translation="MEEFVPFASRSYGLMFLLLAGARGMDFLSTWVATPNLVLEGNPI
                     ARRLGWKWGIPVNLGLCFGFAFWPLPAIVISTTSVLVAARNFQSAWLMRTLGEYQYRE
                     WHLERVRETRMILYLVCLFGQVLLIGGVGAAVIYFSGGQLVPLAIGLGIVAYVLAVAF
                     YSLLGVWRLRRTADLTARLAGRKLVVDAAAPGRNGRVLVAAEPLGPRPWGQ"
     gene            32972..34504
                     /gene="trpE"
                     /locus_tag="PKI07_02010"
     CDS             32972..34504
                     /gene="trpE"
                     /locus_tag="PKI07_02010"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOC49855.1"
                     /translation="MYAPTLDEFLKLADQGNLIPVTCRLLADVETPLSAYRKIRGQGE
                     SFLFESVEGGEHIGRYSFVGCNPRAIIRQVGQTVDVIENGKVIQSFTVQPAAGAAGAP
                     SAEGLRDGLEAVERVMRGYRPVAVPGLPRFTGGAIGFIGYEFIHDIEPVVPRPPRDDL
                     QTPVIYFLIADELLVFDRVAQTITVLVNAVLSDAASPAEAYENAVGEIERLVSLLEQP
                     SEHHPVIVREDVPALPFESNQSREDFLAHVRRAKQYITAGDIIQVVGSQRFSAPARAT
                     ALDVYRAARSVNPSPYMFLLELEGFALVGASPETHVRCEDRQVEIRPIAGTRRRGATP
                     EEDQALEKELLADPKERAEHVMLVDLARNDLGRVCEYGSVQVRDLMIIERYSHVMHIV
                     SQVEGRLAADKTPYDLMRATFPAGTLSGAPKIRAMQVIAELEQTARGPYGGCVGYFSF
                     NGNLDCCITIRSALLKGGRAYIQAGGGWVNDSTPEDEYRETVNKSQAMLKAVAMAESF
                     GG"
     gene            34784..36193
                     /locus_tag="PKI07_02015"
     CDS             34784..36193
                     /locus_tag="PKI07_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOC49856.1"
                     /translation="MGKATDIRVVGAALYFLPVRTRVALKFGAETLTEITCARACVRV
                     TDDRGRTAEGWGETPLSVQWVWPSALAYAERHGALKEFCAELAELWAGMRARGHPMEL
                     GHDFLETALPERLQRFNQRQRRGGEPLPWLAALVCGSPFDLAVHDAYGRLHGCPTYEV
                     YHAGFMNRDLGDYLQAAAPAGVSFAGRYPADFLCERRAESLAAWHLVGGLDLLQASER
                     TGGEPDDGYPVTLEDWIQRDGLKCLKVKLRGTDAEWDYHRLFRVGEIACGQGVDWLSA
                     DFNCTVAGPEYVNGILDRLRDTSPRIYGMILYVEQPFPYDLERHRLDVRSVSARKPLF
                     LDESAHDWRLVRLGRELGWSGVALKTCKTQTGAVLSACWAKAHGMGLMVQDLTNPMLA
                     QIPHALLAAHTGTIMGIETNAMQFYPEASRPEAEVHPGLYRRRQGRIELSSVRGPGFG
                     YRLAEIRRVLPPPAVEFFD"
     gene            36329..39898
                     /locus_tag="PKI07_02020"
     CDS             36329..39898
                     /locus_tag="PKI07_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOC49857.1"
                     /translation="MKRPLRLLHLEDDPDYSDLVRIMLTKEGYEVEVVLAEGRAAFEK
                     ALAPERFDLILADYLLPSYNGLAALRAAQKKCPETPFLLVSGTIGEYAAIESLKAGAT
                     DYVLKNWPEHLVPAVRRAIQEAEERRQRRRAEAELVRRERRFRAITENSLDILTVLDQ
                     EGHFIHNSPSIKRVLGYEPRALIGKSAFALIHPEDLPRVLQAFEAILKTRERPFQAEY
                     RFRHQDGSWRFLEGVGQNRLQDSAIAGVVVNARDITDRKQAEEALRRSEERYRLIFDS
                     NPKPTMVYDHLTLAILEANKAAVQHYGYSAEEFKKLTLRDIRPPEDVPAMIEYMHRIA
                     KAEPNNQVRYAGIWRHRRKNGEIMDVEIKWCPIAFGDRMVSLAMITDITERKRVELRN
                     AAFAKLGQNLNSASSPAEAARIIRSAADDLFRWDAFTLDLYSAEEGRIYPLLNVDTNA
                     RGQRFEDPGTGQPKELSGMARRVVTKGAELILREEPFVIPEDVSPIGDPARPSASLML
                     VPIRNRTQVIGLLSIQSYTPKAYQPADLATLQALADHCGGALERIRAEQALRDSEQRF
                     RELFEGSPDAVFVEDVHGGVLDVNPAACRLHGMTREDLMGRNISELVPPGEQAAAAQK
                     FQELVEGRLAQIEGASYTRDGRIVPVEIRASRITYANQPAVLLHVRDIADRKRAEAAL
                     RSSEMLFHSVWENSADGMRLTDEHGNIVAVNEAFCKLVGLRREELEGKPLTVTEAGPE
                     PPEQRLMEYCQRFREGATEKQKERQLTLRNGQEVILEETNSRVELRGRPPLQLGIFRD
                     VMAQKRLEEQLRQSQKMEAIGQLAGGVAHDFNNILTVIHGHASLLMANGGLAPVHARS
                     AQQIGQAAERAASLTRQLLAFSRRQVMQPRQLDMNEVVSNMTKMLGRILGEDITLQLN
                     YWPQPALVQADNGMMEQVLLNLAVNARDAMPKGGLLAIKVAELEVEAPPGRAHSEARA
                     GRFVCLSITDNGCGIPSENLWRIFDPFFTTKSVGKGTGLGLATVYGIVNQHRGWVEVE
                     SELKRGTVFRVFLPRSTQPAKAPDEPSAPAIVRGGQETILVVEDETPVRELVCDLLRR
                     HGYHVLPAESSAQALQVWQGSKDRIDLVLTDLVMPDQMNGRELVEKLWVERPRLKVLF
                     TSGYSQEVVGKDFVLRRGLNYLQKPYPPHKLARAVRDCLDAVN"
     gene            39970..41469
                     /gene="rpoN"
                     /locus_tag="PKI07_02025"
     CDS             39970..41469
                     /gene="rpoN"
                     /locus_tag="PKI07_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOC49858.1"
                     /translation="MPQGIHLSQRLVQSQVLAPQLQQSLALLQAPVLDLKALVEQELQ
                     QNPVLEEVSPAEAESAETDDRELEASGEAGEPGELSADSDFDAGPGEDTEGPVDAFEA
                     EFERLAQLDQEWRDHFAQTNLPLRTSAEAEEKRQFMFDSLVAETSLQEVLQEQVRDSA
                     LSPEQLPIAEMLIGNIDDYGYLKASLEELAGATGLMVDQIVEVLKVIQTFEPPGVGAR
                     DLQECLRLQLERQGRQNTLEYRIINEFMEALGKRRIPEIARGTGQSVDEVQAALARIA
                     KLEPRPGRAYLPDDDHYILPEVFVRKSDDDYQVTTNNEHVPHLRISNTYKDLMAQRGN
                     PAEVRNYIREKIRAGKFLIKSLQQRQQTILNIAREIVNRQRDFMAKGVIGLKPLTMVQ
                     VAQVVGVHETTVSRAVSGKYMETPQGIFEMKYFFTAGIQTASGDGMSNTSVKDMIADI
                     FKAEDSAKPLSDQEVVRMLKEKGIVIARRTVAKYRTELNILPSNLRKVY"
     gene            complement(41552..43846)
                     /locus_tag="PKI07_02030"
     CDS             complement(41552..43846)
                     /locus_tag="PKI07_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOC49859.1"
                     /translation="MIGFDFIQDLALVMLIAAAAAWVCQRMGVPGVVGYITAGILIGP
                     LAGPHALVSDSGRIQSLAQIGLVFLIFSIGQRVRLQRLKRVGLPLILATAGMAALVLV
                     GCRALGATLGWPAMHSLVLAGMLMVSSTAVLSKSLRQANAVHSGFGQTALTVTTLDDL
                     VAVGMLTVLASVAQAGQTGALLVFGSIIQLKAVMVTMAIGALLLVPSVLKRLGRGIAS
                     EVRSLVAVGLLLAMALLSARAGFSAALGAFLLGSIMSSTDRIGPVERVLEGMCEVLAP
                     VFFVAMGMLFEFKALATTWPLLLGVFGLAVVWRAVAATAALLLAGQPIKEASRAAMCL
                     TPIGEFSLIIALTGVQGGLVPASFYGLAIGVSLLTALTTPVLIRKSEALSEYLEQKQP
                     EFLRQGIGFYHEWIERLKHRRQSSLLWKLAAPQLVQMTCLILFVSGLVIFAKPLYRLA
                     EKWLGTDWPAAGGLPGVCWFGFGLLLLGPLLALWRNVETVALLCAEAAAAGRSKRAEA
                     QFFFERLVKGAALVAILVWLAALFPYGTLPAWEVAGLMAAFLLVGAVFWRKLIRLHNR
                     FEMELRTQLVDSPFAEANGSWAGWPKGNGEGKLNLAESVIGEHTRAVARAISELPLRP
                     LFSCTIASIERQGIVIPNPGAATILYPNDKVLLLGRNEDLRRADRWLSQEAEGAEAAH
                     REPQLSDLSLQRLTVPAVSRHIGKALGELPLKALFGIQIVGIERDQQLVVSPGRSEAL
                     HAGDQLLILGAPEQVNELAFWLST"
     gene            complement(43944..44558)
                     /locus_tag="PKI07_02035"
     CDS             complement(43944..44558)
                     /locus_tag="PKI07_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC49860.1"
                     /translation="MKRRLLVVDDDLSVREAMTELLAAEGYEVLVARDRAEALGHFQP
                     GRVDLVVLDVNLGADDGWEVFQKMSEVDPLVPTVIVTAEHGQQERARAAGVEALIEKP
                     IDVPIFLEIVRDLLTEPGAPPRERVCGSEVYCRYVAQRYETFLRLLQEREGAPMKFSS
                     ELQFLNGIFSLADERPDDEGPLRVIGGAFWGCERKKGSDIGCGA"
     gene            complement(44577..45002)
                     /locus_tag="PKI07_02040"
     CDS             complement(44577..45002)
                     /locus_tag="PKI07_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49861.1"
                     /translation="MKKNLLVISDDPARQDALTALLEREGYGVTRGKTGRPAAALLAA
                     RPVNLVVVDFTTAPNASGRAGHASRTLQALTDVDPFLPLLLLCKREDELDDPTQFMAD
                     MVLTDKAATPVLLDAIETLLSESLRERAQRKAGHVALFR"
     gene            complement(45153..46343)
                     /locus_tag="PKI07_02045"
     CDS             complement(45153..46343)
                     /locus_tag="PKI07_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOC49862.1"
                     /translation="MRVFILGTGATGGLLAQLLRRRGHTVWCGDRDPERARRFAGHAF
                     ECRQANGRSLRSVVRAARGCQLLINAAPAVFNETIMRAALRLRVHYLDLASHLSRNPF
                     KAEQLRFEDDFARRGRLGLINAGVAPGLTNLLVAACADTLDEVDHVRIRLFEDLESDA
                     PVSTWSAEVAHDEATSRPRIYRDGRFRLARRFSEAEWFRFPRPIGKSRVVLAAQDEAA
                     TLPRFIEMNNLDVKIGGNEVDRLRRWYRQGKLRASGERVNERFPDTATPEEVSELMRQ
                     GKLQNARFAVCVVVSGRKGARQVERQRCCVFPSLACLRRARIAGTPIAFATAQCAAVF
                     VEHFPGHLAGVYPPEALSARVRAKILRGMSQRGFRFQRSTTRLATASAGGCRDRGSGA
                     VETN"
     gene            complement(46367..46807)
                     /gene="raiA"
                     /locus_tag="PKI07_02050"
     CDS             complement(46367..46807)
                     /gene="raiA"
                     /locus_tag="PKI07_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOC49863.1"
                     /translation="MKTNVSRLQPRRVRAASRIPVSPWQEDSQNILIRADGVELTDQL
                     RAAVSRKIGRARRYAPRAMRARVQLHKVRGNPSPAQFRVRVHYELPGNDLVAEHTAHD
                     PLAALDIVADKMERQLRRRKTARLVRRVRSQRRKAHTGMPGRRG"
     gene            47210..>47390
                     /locus_tag="PKI07_02055"
     CDS             47210..>47390
                     /locus_tag="PKI07_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49864.1"
                     /translation="MGCWAQIGLVSFAQKKIVAALLPDPSGQFALAVQRIGSDELAIA
                     ETQPPRTKAPMAAGNP"
BASE COUNT         8261 a        14663 c        16106 g         8360 t
ORIGIN      
        1 cagacaaacc cgggtatgaa ttgggcccat cggttaaagg ccgtgcaact cgtgttccga
       61 ccgtaagtgt accaataggt gccgcaatcc gtatgggtgg cccagccacc acttaatccc
      121 gtcggggtct tataggcatc tccccaccac gcccatgccg cgccaggcaa gagcagcagc
      181 agggaaatca aggaaatcca ccctcggcgc cagggaaaag cgcgcccgag cgtatgcgag
      241 ttaaataagg tattcataat catttatggt tggttattta ccctggccgg gtcggcggga
      301 gccgaaacac gtgtttgccc cgccgccccc gtgccaaaga aaaagtgatg ccgattgagc
      361 aggatcgtct cgaaatgctg atgaaagtcg cgcaccgcct cgccgcgcag gtcatggatg
      421 atatagtttc gggcaatctc gtaaggcatc ttgcgccgca actccgactt gcccacgcgc
      481 agcacgcaga acccgccgaa gtcttcatac ggcaccacgg caaccgctcc cgcctccagt
      541 cccgccacca acggagccag cgccgagttc tcctcaacca cgcggctctg gcccggcagc
      601 cattgcaggg ccgcatccgt gctcacaata tcctccaacc gggcgccatc ctgcacccgg
      661 cgaagtatct cctccgcccg gccaaacgca ttcgtccgca ccgcttcccg ctcctccgcc
      721 gtcgcctcgg caggagcccg caccggcact accagggcgt catattctat ctgaggcgga
      781 aaggtcgccg tgaaccgggt atcgtaaatc atgcgcacct gctcctcggg gaaggttaac
      841 tctgcgagca attccggcag caggaaccgc cgcgccttct cggctaattg cttgtccagt
      901 tcgtagcgca aggcccgccg cacctcgggc cactggcggt agcccatgct ccgggccaat
      961 tctcccatct gaatggagtc gcggttctcc tgccggagaa tctcgaccat ctgcgggtcg
     1021 tcaacggttt gcgcgccgcg ttgcaaggcc aggccgcgaa cttcgccgcg cgtgagcgcg
     1081 aactgcccgc aacgcagcaa cacctggccg gctgcggacg gaggagttgc cggggggtga
     1141 acctcggccg gggaagaagc gttggtggaa ctcgtgacga aaggaaacgc agatgccgcc
     1201 gcctgggcca tgtcctgcag ccgctcctgc gccgttattt cgggcagcat caagcgcaac
     1261 tgcgccttga cctcctccgg gctgggattt ccagtgagat gcaggcgcac gatccggaga
     1321 agttcataac catcctcgac cggcacgggc tcgctcactg cgccttcgcc gagctgtccc
     1381 agaaccaacc cattctcttt gccccagaac gtcgccggat agggggccgt ctgcgccagc
     1441 ttgagatcaa ggtccgaata ctgccgggcc actgccacaa agtcctgtcc gttgcgtatc
     1501 aaccccagcg cttgcttcgc ccgttcgagc gccccctccc catgcttctg cgcgctaatc
     1561 ccgatccgcc ctgcctccac ggcctcgaac ccgatcatgt taaaccggtt agtccggtat
     1621 tgttcgtcaa tctcctgctc gctgatcgtc acgctgcggc gtacctcctt ctccagcgcc
     1681 tgcgccagca cccgctgctc gatcagcttc tgctccaacg cccatccagg gccagcctga
     1741 gcatagggtt ggtgctgcgg ctgcggcccc gccttggccg cttccggatc caccggccgg
     1801 tccaaccccc gccgccgggc ttcgctgacc aaaatgcgca ccgccgccag gtgccgggcc
     1861 agcttctcgt ccaggctaac ggcgttggtg ggtgcgttct gctctgcgtc ggccacgaaa
     1921 cgcggttcct ccctggcctc cgccacgtct ccggcactga cgcggccacc gtcataggtg
     1981 gcgatgacat tcgtatcccc tgccggcagg agctccgtag tcgccaagga acggctcgac
     2041 gcttccgcac ccccagcccc agccagggcc aggcaaaaac cggctccaca tacgaacgtc
     2101 accgttcggt gaaaccagcc agtgcccatc aaggagggcg ctgtttttag cagcacagaa
     2161 gccatctttc ggcagccagg atttatcggt gttctctgcc attggctacc aataaaccgg
     2221 cgcctcgatt taattaaaaa agcgcagcgt tgtcaacaag tgatttcact gatttttccg
     2281 cgctgccgtt cacgcatctt gatacagccc cctccggacg ccccccgtct ttaagtggcc
     2341 ggagttccgt caggacagct tggatagaag ggctttctcc atcggtgagg aggcaacgcc
     2401 tcgatcaagc gtgattcgat cttggcgatc gcgtccaaga gtttctctgg ccacagaatc
     2461 cccaggtttc tcaaaaatga gaaaacaagc ccgaattaaa gtcaaaccgt gcctcgtcct
     2521 cccggccagc gaatctccca tccatatctt caatgccaga aatccccctg tttgctccct
     2581 gccaaagcgc cgtgtacaag gggtgtacaa gggatgctca ccagatgtcc atcagatttt
     2641 aggttgtaca cccgatgagc atcccttgta caccccttgt acacggcttg atggtagcta
     2701 tggaagagcc tggaatgacg taggttgggt caattggtgg cggttttggc tgcggggatg
     2761 gtttagcgag gatctggagg gcttgggcaa ggagggggaa tgccttgggg aaccctgttt
     2821 ttggagcgtt ggcgggccaa ggatcgtgag gatgtttgct gtccttgcga gagagcaggc
     2881 gaatggttga tagagacggg gtttagggga gtgtccaata attggacagt ttggggggcg
     2941 gcgttcggtt ggttttgggg ggtggcggtt ttgtggggtt gggttgttta atggccgatg
     3001 gttttgcttt gggggcggtt aggcaggagc ggtttgaggg gacattcggc gcagcggggt
     3061 tggcgggggc ggcaataggt cttgccggtg atgacgagtt gggcgtggta gtcctgccag
     3121 tagtcgagcc gggcggggag gggtttttgg ctcagggcgg attcgcagag agtttggagg
     3181 tcgtcgtagg aggcgtcgcc gcggcaccag ccatgacgtt ggaagatacg ccgggtgtag
     3241 gcatcaatga caaaacgggg gtgcgctccc gcgtagagga gcatgctgtc ggcggtttcg
     3301 gggccgatgc cgtggatggc gagcagccgc tggcgcacgg tggcggtgtc gccggccagc
     3361 aggcggtcga ggcggccgcc gaagtcttcg accagcgttt gcaggaacgc gcgcaatcgg
     3421 cgggctttga cgttgaagta gcccgccggg cggatgagcg ccgccaggtt tctttccggc
     3481 agggcgtaga gtttgcgggg ggtcagcacg cgggcggctt tgaggttggc gatggcgcgc
     3541 tcgacgttgc tccaggcggt gttttgggtg aggatggcgc cgacgcagac ttcgaagggg
     3601 gtttcgcccg gccaccattg caggtggccg aagcgcgcgc gcattcgctg gtaggcgagt
     3661 cgcagggcct gggcgcggcg cgcggccggg ttgggggcgg ggcgggtcat gggggggtct
     3721 gtccggtgag gccggccatg gcccggcccg caatccagcc ggtggtccag gcggcctgca
     3781 ggttgaagcc gccggtcagc ccgtcaatgt ccaggagttc gccgccgaag tagagccccg
     3841 ggcaaaggcg gctttccatg gtgtggaagt tgacttcgcg caagcgcacg ccgccgcagg
     3901 tgacgaactc gtcctggttt gggctgcggc cgttcacttg cagttcggtg gcgcggaggt
     3961 gttcgatgag ctgatgccgg gcggcgcgcg gcaggccggc ccagcgggtt tcagggggga
     4021 tgccggccgc ctgcacgagg cgctgccaga gccgggcggg caagggggac aggggggagt
     4081 ttgcgatgcg gcgcgccggt tccgcggcgc ggcggtggtc cagttgggcg gcgagggccg
     4141 ccgggccgag atgcggcagc cagttgacgc gcagcgggaa ctggtagcgc gccgcgtgca
     4201 gcgcccgggc gccccaggcg gagaggcgca agatggccgg gccgctcagg ccggtgtggg
     4261 tgaagagcag ggggccgcgt tcgcgcaagc ccgggccggg gagcgagagt tccgcgtccg
     4321 cgacggcgac gcccgccagt tcgcgccccc acggggtggc gaggcgaaac gcgaagagcg
     4381 agggcaccgg gggttcgagg gagtggccga gggcgagcgg caggctgccg tccgcggcgg
     4441 tgcggcagcc gccggtggcc agcatgagcc gttcgcagtc gaggcgttct cccccggcca
     4501 gcgcgagggt gaagccgccc gccggccggc gcgcgagggc cgtgacttcg cagcggggcc
     4561 gcagggtcac gccggcggcg cgggcggagc gcgtcagaca ttcgaggatg gcgcgcgagg
     4621 aatcgccggc cgggaacatg cgcccatcgg gctcgatttt gagtttcacg ccgcggcgtt
     4681 caaaccaggc gcgggtgtcc cggggctgga agcgggtgaa ggggccgcgc aaggcggcgc
     4741 ccccgcgcgg gtaatggcgg ctgaggtccc ggggttcgaa gcaggcgtgg gtgacattgc
     4801 agcgtccgcc gccggagatt cgcactttgg cgaggaagag cgggcttttt tcgagcacgg
     4861 tgacccgggt agcgggggcc gcttccgcgc aggtgatggc ggcgaagcag ccggcggcgc
     4921 cgccgcccgc gacaacgacg tgcggggggt tcattccggc agcgcgcgca cggtgtagcc
     4981 gcctgatttt tcgtccgcct ggagttgcag caggccgcgt tcctgggctt tctggagcag
     5041 gtcgttaaag gagcggaacc cgtaggcgcg ttcgttgaag ccggggttgc ggcgtttgat
     5101 ggcctgcttg atcatcgagc cccagatggg ttcgtccgcc tcgcgctcct ggctgatggc
     5161 ctcgagggtt tcctccacca gctcgaaggc tttttcccgc gagggcccgg cgttggtttc
     5221 ggcggcgggt ttggccgccg gggcgctggc cgcggcctgc cgccgcgccc ggggcggcgc
     5281 ggcgcggacc aggtcgtcgt aatagatgaa ttcgtcgcag ttgctgataa agaggtcggc
     5341 ggtggagttc ttgactccca cgccgatgac cgttttggcg ttctcgcgca gcttgctgac
     5401 cagcggggag aagtccgagt cgccgctgat gatgacgaag gtatccacat ggcccttggt
     5461 gtagcacagg tccagcgcat ccacgaccat gcggatgtcg gcggagtttt tgccggattg
     5521 gcggacgtgg gggatttcga tgagctcgaa ggcggcttcg tggaggtcgc gtttgaacgt
     5581 tttgtagcgg tcgaagtcgc aataggcttt cttgacgacg atgtggcctt tgagcagcag
     5641 gcgttcgagg actttttgga tgctgaacgt cgggtagcgg gcgtcgagcg cgccgcgcgc
     5701 gatgttttcc aggtccaggt acaccgccat ggtcgcattg ggagcttggg ttttcataat
     5761 cgtggtcatt caggccggcc ccagattcgc gcatcgcggc gggcgaagcg agcgaaaaag
     5821 gcgggcgggg gcgcggcgcg ggcgggcgag ggcgggggcg ggcttccgcc ggcggccgga
     5881 ataaagcggg cttttccccg ggggcggcgt tatgctgggg gcatggcgaa gagattgttt
     5941 ttgctggacg gcatggcgct ggtgtaccgg gcgcactttg cgtttatcgc ccggccggtg
     6001 ctgacgtcgc agggggtgaa tacgtcggcg ctttacgggt tcacccagac gctgctgaac
     6061 atcctgggcg gctgggagcc gacgcacctg ggggtggcgt ttgacacggc ggcgccgacg
     6121 caccggcatc gggagtttgc cgcgtacaag gcgacccggc aggagatgcc ggaggatctg
     6181 agcgccgcgc tgccgcacgt gcggcggatg ctcgaggcgt tcaatattcc cgcgctggcc
     6241 ctggatggct tcgaggcgga tgatctcatc ggcacgctgg tgcggcgcgc ggagcgggag
     6301 ggcttccagt cctacatggt cacgtcggac aaggatttcg ggcaactggt cagcgcgcac
     6361 aattttatct tcaagccggc gcggggcggg gacggggcgg agattctggg gctggcggag
     6421 atccaggccc ggtggggcgt gtcgcgcgcc gagcaggtgg tggaggtgct ggcgctgatg
     6481 ggcgacgcgt ccgacaacat tccgggcgtg cccgggatag gcgagaagac cgcggtgaag
     6541 ctcatcgcgc agtatggcac gctggagaac ctgctggccc atgttggcga attgaccggg
     6601 cgcgtgcggc aggccctgga ggcgcacccg gagcaggcgc ggcaatcccg gcggctggcg
     6661 accatccggt gcgacgcgcc gctggagctc gaactggacg cgctccgggt gcggccgcgc
     6721 aacgaggaaa agctccggga gttgctggcc gagttcgagt tcaacgcgct ggggcggcgg
     6781 ctgttcgggg aggagtttcg ggcggggcgc gcgcggggga gcgtcccggc gggggcgggc
     6841 ggggcgcggg ggcgggcggg cgagcagttg gtgttgctgg gggagccgga ggcggcggcg
     6901 gcgaaaccga aacccggggc ggcggcgcgg cccgggctga agacccttgc ggaggtgccg
     6961 caccagtatc acctggccgc cacggcggcc gagcgcgcgc aactcatccg gaagctgcag
     7021 gggctggatt ccttctgcct ggatatcgag accaccagcc tggacccgaa ggcggcgcgc
     7081 ctggtggggc tggcgttcag ctttgcggcg cattccggct attacgttcc cctgccgccg
     7141 gcgccccggg cggcgccggt gctggaggag tttcggccgg tgctggagtc ggagcggatc
     7201 gagaaagtgg ggcataatct caagttcgac ctgagcgttt tgaagtggca gggcatttcc
     7261 gtcggcggga agctctttga caccctgatt gcgcacagcc tgctggagcc ggagatgcgc
     7321 cacggcatgg atcacctggc cgaggtttat ctggactaca cgcccattcc gattggccgg
     7381 ttgctggggg attccggggc ggcgcgggcc ggcctggccg acgtgcccgt ggcccaggtg
     7441 gccgagtatg ccgccgagga cgccgacgtg acctggcaac tgcgcgccgt cctggagccg
     7501 ctgctgaagg cccgggggca ggagcgggtt ttttatgagg tggaagcgcc gctgattccg
     7561 gttttggtgg acatggaata cgagggcatc aaggtggatg ccgcggcgct ggccgagttt
     7621 gcggcgcagt tggccgggga gatgggcgcg gcggagaagg cgatttaccg gctggcgggc
     7681 accccgttca acctcaattc tccccggcag ttggggcaga ttctgttcga cgtgctcaag
     7741 ctcggcgcgg cgccccggaa gacccggacg ggccagtatg ccaccgacga gcaaaccttg
     7801 caggcgctgg ccgccgacca tgagattgtg cggcggctgc tggagtatcg cgccgccgcc
     7861 aagctcaagt ccacgtatgc tgacgccctg ccggaggcca tctggcccgg gacggggcgg
     7921 gtgcacacca cttatcacca ggtgatgacc gcgaccgggc ggctcagttc gcagaacccc
     7981 aacgtgcaga atatccccat ccgcacggag cgggggcagg aaatccgcaa agcctttgtg
     8041 ccgcgcggcc ccgagtatcg gctgctggcg gcggactatt cgcaaatcga gctgcggata
     8101 ctcgccgcgc tgagccgcga gacggtcatg ctggaggcgt ttcggagcgg cgcggatatt
     8161 cacacggcga ccgccgcccg ggtcttcggc attccgcccg gggaggtgac gcccgagatg
     8221 cggcgcaagg ccaagatggt gaacttcggc atcgcctatg gcatttcggc gtttggtttg
     8281 tcccagcggc tgggcatttc gcgcgccgag gcgggcacca tcatcgcgca atacttccag
     8341 cagttccccg gcatccggcg ctaccgggac gagacgatcg cccgggcccg cgagcggggc
     8401 tatgtcgaga cggtcaccgg gcggcggcgt tacctccggg atattcgttc ggccaacgac
     8461 accgcgcgcg ccgccgccga gcgcaatgcc atcaatgccc ccatccaggg cagcgcggca
     8521 gacatgatta agctggccat ggttgccatt caccgggaac tggtccgccg ggggctgcgg
     8581 agccggatgc tgctccaggt ccacgatgaa ctggtgtttg atctttacct gccggagcgg
     8641 gaggaagtcc ggcggctggt cggggagatc atgacgaccg ccctgccgct ggaggtggcc
     8701 atggcggtgg agatgggcga gggggaaaac tggctggcgg cgcattgagg cggctttcgg
     8761 acagcgacgg gcggcggcgg cgcgcggcgc caagaacacc cttgctcctg gttggcggct
     8821 ttggctacgt ttgcgcctgc ggaatcgcag catgagtcgc cacccaaaga catgggccca
     8881 accggatccg gcgtgccgcc tgccagacca ggcgccgggg gcgcgcgcgg ctgtctggac
     8941 ggggggcggg cgggcgcggc gcggttgggg cggggggggc ggcgtggcgg cggtggtcct
     9001 gatcgggttg ctattgagcg gctgcaccgc ccggcattac cggcggtcgg cggacaagga
     9061 gagctacgcc attatccagc agatggagcg gcgggttttc gggcatacca atgaattttc
     9121 gatccagaca cggtattccg accgggatcc gaagactatt tctcccgcgg aggttctgga
     9181 tgatcgcgcg gtgagcaacc gctttttcgt gaacctggac caggcgttgc aactggcggt
     9241 ggagaacagc cgcgagtatc agacgcaaaa ggagcaatta tacctggcgg ccctggcgct
     9301 gacgggcgcg cgctacgagt tcagcccgca gttctttgcc caggccacgc cgcagattgc
     9361 cggcagcccc gatggcgcgg acgtggggag cgtggcgagc aaggtggggg tcaaccagtt
     9421 gctcaagacc ggcggttcgc tgacgatggc gctggccaac gacctggtgc ggtattttgg
     9481 cggccagccg gcggcgacgg cgcggcaatc ggtcatcaac gtgctttcgg tgaacctgac
     9541 gcagccgctg ctgcgcggtt ttggcatcaa cagcccggcg gtggaggcgt tgacgcaggc
     9601 cgagcgcgac gtggtttatg ccgtgcgcac ctacagcctg taccagcagg agttcgcggt
     9661 gggaacggtc agcgcgtatt tcgggctgct gacgcaaaag gatattgtcc ggaacaatta
     9721 ccgcaactac accaaccgcg tggagacgac ccggtatctg caggcccggg ccgtggaccg
     9781 cgagcggctg agcaacgcgg atgatgcgcg cacggccgaa ctgaaggccc ggacggatta
     9841 tattgactcg ctggcgtcgt atttgagcgc gctggacgcg ttcaagctgc ggctgggcat
     9901 cccgctggcg gcgcaattat ttctggacga ccgcgacctc gaggagctga tcggggcggg
     9961 gctgatcccg gttgacctcg atcgccgggc ggcgtttgcc atttgcgtgc agagccagat
    10021 ggaggtgctc aacgccattg accgcttcga ggacagcaag cgcaaggtgc gggtggcggc
    10081 cgaccagttg cgggcggatg tgaatcttat tgggagcgcg agcctcgcgt cggagcctcc
    10141 ggacgattat acgaacttcg atccgggcaa ggtgcagtat agcgccgggg tgcgccttaa
    10201 tttgccgctg gatcggctgc gcgagcgcaa ccagtaccgg aggtcgctgg tcgcgttcga
    10261 ggcgcaactg cgcgcgctga gcctgacgct ggacaacttc cgggatcgcc tggatcgggg
    10321 gctgcggacg ctggagcaag cccgcctcaa ccatgtcagc gcggcggaat cgttgaaagt
    10381 cgcgcagcgg cgggtcgaga acaacctcat gctgatggag gccgggcgcg ccaccatccg
    10441 cgatgtgcgt gaggcgcagg acggactgat ccaggcgcaa aacagcctgg ccacgattta
    10501 cgcccagtat ctgacggcgc gcctgacgct gctgcttaac gtgggactca tagacacccg
    10561 gccggagcgg ttctggctgc aggacccgct gcgcgagcgg ttgacgccgg agcagcgcgg
    10621 gccgtcgccg ctgcgaatgc cggacgacca ggtgttgccg ccggaaaact tcttggaacc
    10681 gacttcatga acgcgagtaa tcttttggac aaacttcccc cggtgctgcg gcgccgtccg
    10741 gttctgttcg gggccgccgg gctggtgttg ctggtgctgg tcattctgtg gctgcgcccg
    10801 gcccggcccg cggtggagcc cacggcctac tacgaggtca agcgcgggga tttcaccgtc
    10861 tccgtggtcg agggcggcac gctttcggcg gtgagcgagt atagcattcg cagcgaggtg
    10921 gagggcaccg cgcgcattat ttccatcgtg ccggaaggca gctatgtgaa gaagggcgac
    10981 ctgctggtcg aattggattc cgcctcggcc caggaccagg taaaccagca gcaaatcaac
    11041 tacgagaagg ccaagttcag cgtggagcag gcgctggcgc aattggagat acagaagagc
    11101 gcgaccaaca gcgagtatct ggcggcggaa ctcaagctga agctggcgcg gattgacctg
    11161 gaaaaatatg agcagggcca gcggttggtg gacctggtcg aggccagcaa caagctcgtg
    11221 caagcccagg cccagttaat ggtgaacatg gatacgtacc gcaattccac caacctggcc
    11281 gccaaaggat atgagaccaa gcagaaggtg gatagcgacc gcctggcggt gctcaactcc
    11341 gccaatgcgc tgatcgtggc cagcaatagc atctggatgc tgcggcagtt tgacgaagtc
    11401 aagcaggccg aaacctaccg ggcggcggtg ctgcaggccg agcaggagct ggaccgggtg
    11461 gtgagccaga acgtgcgcaa aatggcgcag tattccgccg acctcatcac ccaatccaac
    11521 acgctggcgc tcagcgaaca gaaactggag cgcgacaaga agaacctggc ggcgaccatc
    11581 attcgggcgc cgcaggatgg gctggtggtc tatccggtca gcgacagcca tttcagcagc
    11641 gaatccctga ttgaaggcgg cgccatcgtc cgcaaccggc aggagttgat caagctgccg
    11701 gacctgtccc gcatgaaagt ggtgatcaag gtgcatgaat cccacgtgaa catgattcgc
    11761 cccgggcttc cggcgtttgt catgctcgat tcgatgccgg acgagcggtt tcggggcgtg
    11821 gtggaaaaag tcgccccgct gccggacacg caatcgcgct ggggcaatcc caacctgaaa
    11881 gtctataaca ccgaaatcta cctgaccgac accctgccga acgtgaagcc cggggtctca
    11941 gccaaggccg aaatcatcgt gacgaacata gccggggcca tttcggttcc catccaggcc
    12001 atcgccacct accagggcca gccggcggcg tacctcgtgc atgggcatcg ctccgagccg
    12061 cgccccgtcg aggtggggat gttcaacacc aagtttatcg agatcaccaa ggggttgaag
    12121 ccgggggatc gaatcttgct ttcgccgccg tttgaggcgc gggaaaagga tttggaaggc
    12181 agcgttctgg cggagggtga gaaggccggc gcggccacca atgctccgcc acggacaacg
    12241 ccgcccgcgc ccgcgcccgg tttgtcttcc ggcgagcccg ccgccggcgg cgaggagcgc
    12301 gccgccgaca gccgccgcgc gcgcggcaat ttcaacccgg aggagatgct caaacagttt
    12361 gacaagaatg gcgacggcaa actggacgac gacgagcgcg aagcgatgcg cgcggccatg
    12421 gccgcccgcc tcggcggcgg gtcgggcgcc agcggcacga atgccccgcc gaggttgagc
    12481 ccggaagagg tgctgcggag atttgataag aacggcaatg gcaagctgga tgaggacgag
    12541 cttgccgcca tgcgcgagag cctgggacgc gggcgcgggg ggctgggaaa aggcccggga
    12601 gccagcggcg ccgggaacgc cggaggcgac gcggccgccc cgcgccggca attgcccgcc
    12661 ccggccaaac catgagttcc gttccgccga agccctcttc cgcgcgtcgc ccctccggcc
    12721 gggccggggc ggcggcgggc acggcgttcg gagagagcat catccgcctg gaagcgttgc
    12781 agaaaacgta tgtgctgggc gaagagaata tcgtgcgcgc ccttgacggg gtggatttaa
    12841 ccatccatgc gggctcgttc gttgccatca tggggccctc cggctctggc aaatccacga
    12901 tgctcaatat cctgggctgc ctggaccggc ccacggccgg acggtatttt ctcggcaccg
    12961 aggatgtctc ccgcatgagc gacgacgccc tgtcggaagt tcgaggccgc cgcatcgggt
    13021 tcatctttca atcctacaac ctgatcgcgc aactgaccgt gctggagaac atccaggtgc
    13081 cgctctttta ccgcgggcag gatatcaaga cttcccgaga ccggtgcgtt gagttggcga
    13141 aactggtggg gctgggcgac cggctcacgc atcggcccag ccagttgtcg ggcggccagc
    13201 agcagcgcgt ggccattgcc cgttccctgg tgaatgatcc gctgatgatc ctggccgatg
    13261 agcccaccgg caacctcgac tccaagaccg gcaccgaggt gctggagttg atcagccggc
    13321 tgaatcagga cggcaagacc atcgtactgg ttacgcatga cgacaaagtg gcggagcggg
    13381 ctcagcgggt tgtgcgcatg aaagacgggc gaatagatcg ggacgagcag cgggcgccga
    13441 acgcaaccgc ggccccggcg cgttgaggcg atgattcctt accaattcct catcaccgtc
    13501 cggcttggct tcaaaagcct caagctgcat aaactgcgct cggcgctgac catgcttggc
    13561 atcatttttg gcgtttgctc ggtcattgcc atgctggcga tcggcgaagg ggcgtcctac
    13621 gaagcgcagg aggccatcaa gcggttgggc agcaataaca tcctgattcg cagcctcaag
    13681 ccgcccgaac aggcccgcca gcagggcgcg ggcagccggc gcggtttcca gataaagtac
    13741 ggccttactt acgacgatgc gggacgcctg ctcggcacca ttcccggcgt ttcccgggtt
    13801 ctgcccatgc gcatcatccg cgaaaatgtc cgctttcaac gcactgaaat cccctgccag
    13861 gtcatcggca cgcttccttt ttatcccgag gtggctgggg cgacgttggt gcgtgggcga
    13921 ttcctggcat attacgacga agagcacatg gaaaatgtgt gcgtcattac caccgggctg
    13981 gcgcgccagc ttttcccgta tcaggacccg ctcaaccatg cgataaagat tgccaccttt
    14041 tactatcggg ttattggcat cgttcaggag cgcagccagc cggaacagcg cagccaggct
    14101 ggcaaaatgg aagggcagcc gcttgataat aacgtttata tccccttgaa cacctcccgc
    14161 acccggttcg gcgaaatcct ctttcgccgt tccgcgggca gttttgaggc ggaagaggtg
    14221 caactccatc aggtcaccgt ccagatgcgc gacctggcgt cggtggaaac cgccgacccg
    14281 caaatccgga ccctgctggg ccgctttcac gaggacaacg attatgaaat cattgttcca
    14341 ctgcagttgc tccggcaggc ggagcagacc aagcgcattt tcaacattgt gctggggtca
    14401 attgccgcga tttcactgct ggtcgggggg atcggtatta tgaatatcat gctggcaacc
    14461 gtgaccgaac ggacccgcga aatagggatt cggcgcgccc tgggcgccaa gaaagcggat
    14521 attaccatgc aatttctggt tgaaacggtc gtgctcgcgg ttgggggagg gctggtcggg
    14581 gtggcggttg ggatcattac tcccgttctg gtctcgcatc tcaccacgat gaagacgatc
    14641 gtcaccctgt ggtccgtttt gctggccttt ggcatctccg gcgcggtcgg cgttatcttt
    14701 gggctttacc ctgccaaagc tgccgcggat ttggatccca ttgaggcgtt gcgccacgag
    14761 taattgaatt gcccgcataa aggcaagcgc tggcctgcct catttcacgg gccgggatag
    14821 ctgcttcatc ctcctctttt cttattagct ccggccccat taccgtagct tcgcggttgc
    14881 gggtgagagg cgctgtcgtg atggggcagc gcgggcagga agtggcgaaa gcatggggcg
    14941 gataaaggga gaggtggaaa gccgggcgcg agcaggcgca tgccgccgtg agggacgaaa
    15001 tggccgggag caaacccgcg aggctcgcct ggtttcggtc gagggaagga gtcgggaatc
    15061 cgtaatccgc atcgcgactg acctttggag cacccaaagc cattaccgcc caaaccccgg
    15121 ctttgcgccg ggccccaacc actgcttcgc cgtgtggttg gggtggtccg ggcttgtctt
    15181 ggggggcgga ttggtttact gctgagcaga tggaaattgc ccgagatgct cggatttatg
    15241 tagcggggca ccgcggcttg gtgggcagcg cgatctggcg cgaattgcaa cggcgcggct
    15301 atacgcacct gatcgggcgc actcgcgccg aattggatct gctggacgga gccgccgtca
    15361 atcggttcta cgcgcaggag aagcccgaat atgtggtgga tgcggcggcc aaagtgggcg
    15421 gaatcctggc taacgaccgg cacccggcgg agttcattta ccagaacctg caaatccaga
    15481 accatctcat tcacggcgct tacgtggccg gggtgcgcaa gctgctgttc ctgggcagtt
    15541 cctgcattta tccgcggctg tcggtgcagc ccatgaagga agagtatctg ctgaccgggc
    15601 cgctcgagcc gaccaacgag tggtacgcgg tggcgaagat cgccggcata aagatgtgcc
    15661 aggcgtatcg gcgccaatat ggctgcgatt tcatcagcgc catgcccacc aacatgtatg
    15721 ggcccaatga caattatgat ccgcaaaact cgcatgtgct gccggccctg atccgccggt
    15781 ttcacgaagc caggcaggcc aacgctccca cggtgacctg ctggggcacc ggctcgcctt
    15841 tccgggagtt cctgtatgcg gatgacctgg cccgggcttg tatttttttg ctcgaacatt
    15901 acagcgagga gcaattcatc aacgtcggtt ccggcacgga agtttccgtg cgcgaactga
    15961 ccgaaacggt tgggcgggtg atcggctatg ccggccggat cgagtgggat gccagcaagc
    16021 ccgacggcat gccccgcaag ctgatggaca gctcgcgcct gtttgcgctc ggctggaagc
    16081 cccaggttga tctcgaaacc ggcatccgcc tggcctacga ggattaccgg aagcgattcg
    16141 gctgaaacgg cttcggagca cagccatggc atcccggcgg gtcgtcatta tcggcggagg
    16201 catcgtgggg ctggccgcgg cgcataaact ggcggcgcgt tggcccgccg cgcgcatcct
    16261 gctgctggaa aaggaagggc gggtgtgcgc ccaccagagc ggccacaaca gcggcgtcct
    16321 gcattccggg ctgtactacc agccgggctc attgaaagcc cggctggccg tggcgggcat
    16381 tcgcgagatg gtggcctttt gccaggatca tgcggtgccc cacgaggttt gcggcaagct
    16441 ggtggtggcc gcggatgacg ccgaggtgcc gcgcctgcgc cgcctgcagg agcggggtgc
    16501 ccaaaatggc ctggaggggc tgcgctggct caatcgcgag cagatgcgcg aggtcgaacc
    16561 gcatgtgggc ggagtcgccg cgttgcaggt tccccaggaa ggcatcgtgg actacccgaa
    16621 agtctgcgaa gccctccagc gggaaatcga agcccgcgga ggctccgtgg tggtgggggc
    16681 gcgggtgacg gcgctgcggc tgcggaacgg cgagtggcgg atcgagagcc caaaggggga
    16741 ttgggcggcg gacttcgtga tcaactgcgc cgggctgcat tgcgaccgtg tcagccagct
    16801 tgccggtgag aaacgcgagg tgcgcatcgt gcctttccgc ggcgagtatt acgcgctcaa
    16861 accggagcgg gaacacctgg tgcgcaacct gatttacccg gtgccggatc cgcagtttcc
    16921 ctttttgggc gtgcatttca cgcggctcat tcacggcggc cgggaggcgg gccccaacgc
    16981 ggtgctggcg ctcgcccggg agggttaccg gaaaacggac gtgcggctgg ccgacgtgtg
    17041 ggacgccctg accttttccg gcctgtggcg ttttttggcg cggcacaaac gcatgagctg
    17101 ggacgagttg cgccgctcgt tcagcaaacg gttgttctgc gcctcgctgc agcggctggt
    17161 gccggaaata caggaggccg acctggcgcc gggcggcgcc ggcgtgcgcg cgcaggccat
    17221 ggcgccggat ggcaccctgg tccaggactt ctgcctgctg caacggccgc aggcgctgca
    17281 cgtgctgaat gcgcccagcc cggccggaac cgccgcgctg gccatcggca gcgagatcgc
    17341 aaatctggtt gccagcgaaa gctgacttgc tctacttaat gctccttttc tgactatgaa
    17401 aatactgatt accggcggcg ccggctacct cggctcggtg ctgacgccaa ccctcctggg
    17461 actggggcac cacgtcacgg tggtggacaa ctttctcttc cggcagaaca gcctggcgga
    17521 ctgctgcccc tacgagggat ttcagatcgt gcgcggtgat tgccgggatg aggcgctcat
    17581 gaagaaactg gtgggcgggg cggatgtcat catccccctg gcggcgctgg tgggggcgcc
    17641 tttgtgcgat cgcgatcccg tggccgcgcg caccacgaat ttcgaggccg tggccatgct
    17701 gtgccgcctg gcttccgcgg cccagcgcat cctcatgccg gtgaccaaca gcggctacgg
    17761 cgtcggcgag aaagacaagt tctgcacgga agaaacgccg ctgcggccca tctccctgta
    17821 cggaaccacg aaggtgcagg ctgaggaggt catcctgcag cgcgagaata gcatcagctt
    17881 tcgcttggcc accgtttttg gcgtctcgcc gcgcatgcgg attgatctgc tggtgaatga
    17941 tttcgtccac cgggcggtca acgaccgcgc ggtggttatc ttcgaggggc acttcaagcg
    18001 caattatatc cacgtgcgcg atgtggcgcg cgtattcgcg cacgcgctca accgcttcga
    18061 ggagatgaaa ggcaagccct ataacgtcgg gctggacgag gcgaatctct ccaagctcga
    18121 actgtgcgcc gtcatccaga agcacctgcc gaagtttgtc tttctggaag cgccgattgg
    18181 cgaagacccg gacaagcggg actacatcgt gtccaatgcg cgcctggcgg cgacgggctt
    18241 cacgtcggag tgggggcttg accggggcat tcgggaactg atcaaaggat tcaccatcct
    18301 gcgcaacagc gtgtacgcca acgtctaggc gcgggcatga cgcgggaact gagccagaca
    18361 gtggccgtgg tgctggcggg cggcctcggg acccgcgtgg cgcacttgct gccgggggtg
    18421 cccaaaccca tggccccggt atccggcaaa ccatttctcg aatgggtggt gcgctggctg
    18481 gcgcagcagg ggattcggaa ggtcattctt tccaccggcc acctggccgg agtggtggcg
    18541 gcgcatttcc aggcgcaacc ggttcccggc gtgctcacgc gctgcctggc ggagccccag
    18601 cccctgggga cggccggcgg cttcctgcat gcggcgcgcc tttcggcgga aacgccgccg
    18661 gcctggctgg tcgtcaatgg cgactcgctg gtcctcgcct cgctggcggc ggcggcccgg
    18721 gagttgcacc gccaccccgc ggttgccggc gttgtggtgg ggcgcgccgt gccggatgcg
    18781 gcgcgctatg gggcgctgac gctcgggccc ggcgccgagt tgcgcgggtt tgcggagaaa
    18841 cagcccgggc cgggggtcat caacgccggg atttaccttt tgcgggacac gctggtgcgc
    18901 caattacccg gccagtggcc gctgagtttc gagcaggagg tgtttccgcg gttgctggcg
    18961 caaggctgcg cgctcaaagt atgcgtggtg gaagcgccgt tcctcgatat cggcacgccc
    19021 gaatccctgc ggcaggccga ggcgttcgtc cagcaaaacc gcgcgcagtt tggcgattag
    19081 agtgacgatg atgaagaagt caggcagcaa tcgggaggcg gtgttcacct cgccctggtt
    19141 ccaggttttg gccgaatcaa ccccgggcgg cggcccgccg cattatgtca ttcaggggct
    19201 cgatttcgtg gtgatcgtgg ccctgacccg gcaggatcaa ctgttgctgg tgcgccagtt
    19261 tcggcgggcg gcgggggcga tcacgctgga gttgcccgcg ggccacattg acccggggga
    19321 aacccccgag caggcggccc ggcgggagct ggccgaggaa acgggctatg aggcggatca
    19381 tttcgaattg ctggcgagcc tttctccttc agtcgcccgc ttcaccaacc gcatgtggtg
    19441 ttttctggcg accggagccc gcccggccac cagcccgcaa accgaacgcg aggcgggcgt
    19501 ggacctgatc ttgtatgacc ggggggttca ggagttgatt ggagagaagg atttttacag
    19561 cgcgcccaac tacgcggcac tgctggtcgc cgtgttgcgc gggaaactga aaccccggcc
    19621 cgatctccac cgttagcgcg caattcccgc cgcgggtggc ggcggccggc attcaatgat
    19681 ggggcccggc ttgcggcgct cacccgagcg cgatctcatc cgccgcgggc ggttcggggc
    19741 cgcccccgcg ggaggcatca tcgggcgggc gcggacgcca gccggcgatt gagggtgtgc
    19801 aaccccgcca cgacgaacgc gtccaattcc cgtgagaacg ccgtggcgcg ttcgggttga
    19861 gcggaggcga gatcggcgga agcgctgggc gggaaaccga ggcccagccg gtaaaagtgc
    19921 gccggcgcat ccaggctggc ggtcaggatt tcgtcgccct ggatccagcc gaggatcggt
    19981 tcgcctacgc gtttgacata agcctggccg gggtcatccg gcggcaggcg gaacaaatcg
    20041 cgcccgaagg cctggtgcgg cgtgtccagc ccgaggattc ccaccaccgt gggcaggatg
    20101 tccaactggc tggcaacctg gtggcgcacc tcgtgttggc cgccaaataa ttccgggccg
    20161 taaaagagca ggggcacgtg catatgcagg aggcttgtct ccgtaaggtt gggcggaatg
    20221 ccgaaaccgt ggtcgccgac aaagacaaag agggtgtccg cgaaccactc ctgtttgcgc
    20281 gcggacgcca tgaactggcc gagcgcccaa tcggcgtagt gcaccccgtt gaggcgcttg
    20341 ttttgttcgc cgccgtccgt gatcggttcc agccccgcga ctggaggcag gtcaaacggc
    20401 gcatggttgg acagcgtgag aatgatgccg agaaatcgct tcttcccggc ggacagggcg
    20461 gagaattcct ccacggcgcg gttgaatacg tcaaagtcgc aaggccccca gtccgggtcc
    20521 acaaaggtgg gattcttgaa gtcgttccgg ccaatgaacc gttgcacgcc ctggtgccgg
    20581 taaaacccct ccttgttgtc ccaggagaag aggccgttgt aaaggaagag cgtggcaaag
    20641 ccgcgttcct gaagaatccg gggcagggag cggaagggtt gctcgcccac cggccccttg
    20701 gccgggcggt cattgtcggg caggttggga aacgagcata gcgtggcgaa aacgccctgc
    20761 gccgtgtgcg tgctgatgga aaaagcgcgg tcgaacaaaa taccctcgcg cgccagcgcc
    20821 gcaaaacagg gagtcgcgcc cagggaagcc ccgagggcgt cattgaaccg cgcggtgaaa
    20881 ctctccatga tcaccaccac cacattgcgc gggcgccgga cgaccgcctc gttgggcggc
    20941 gacacccgca ggagcgggta tttcccggcg tccaccagtt tctcgcccgg cagcaaaatg
    21001 ctttcccgca ctttctggat ggcctggtcc agcggcatcc gccgctgcca ctgccgggtg
    21061 gatttctgag ttgcggagga atgggagccc agggaatcca gcaaactcca caggccgttc
    21121 agtgtcattt ggttcacata ggtgctgggg gaaaagtagg cattgcccca gcgcaaggga
    21181 gtgtgctgga ggccgccgcg gatgcccacc acggccacgc ccgccagcgc ggcgacaacc
    21241 aggaggcgcg ggagccagcc gacctggccg ggcgccggct gaaagagacg ccgggtggcc
    21301 cagagaaaca ccgcccagag agccaggcat accagcgtcc agcggaccac gggatagcca
    21361 tgccagatca tgcccaggac aatcgcgtta tgctcggcct gggtgctgaa atactcgtac
    21421 gccagggggc caaggcgcat ctggaactct ttgtaaaact ccacctccgc gatcagcgca
    21481 aagagccccg ccagagccag gagggcatag atcgcaaaag ccaggccgcg ctcgcgccgc
    21541 ccgggcgccg cgcgcaggat ccgccacaac gcaaagggaa gcgtaagcca aacagccgtg
    21601 gccacgtcaa agcgcaggcc gtgccagaac gacagcccaa tcagcggcat cggcgcccct
    21661 ccgcgatgcg accacgtggt gataatcaaa agcccgcgca acagttggaa ggccaccagc
    21721 cagataacaa agagcccgaa aaccggccgc agcattttcc agatcgttac gagaaacgaa
    21781 gccggaacaa cgggggacgg gagagaggcg ccggaggagg gctggcgctg acctgcgtca
    21841 gaggtatccc cgcccggcgg ttcagatgag ttcatagaaa ggtttactgg gatgcgacag
    21901 cacgggatat ccgcgcgttc aatttgcgcc aatcaggcag tgtgggttga cgggtgttcc
    21961 tatggggctg gctacggctg atgcgccggg cggccaggct tgaaatgctt caaaaagcag
    22021 tgaattgcca cccaggcggc aagaatgcaa acgaaggccg ccaaggccag tttgaagaac
    22081 ttctcgaaac ttgaatggag catcccggcc cccagcagaa ccaggggcac cgcttcgggg
    22141 atcagcccca ccagcgaacc cgccagatag accccggtgc ggacattgct caaggccagg
    22201 gctacggtgg ccaccatgcc cggcacgggc aacatgcgcg aaacgatcac cgccgccagc
    22261 cccggctgat ccgccagaaa agccagcttc ggatgcaagg gcggggcctg attcgtattg
    22321 tgccgccgcc cccgaatgac caggaaagcc gcataataac tggccattgt gcccaacacg
    22381 ctcatcgcca tcccgcccca gaagccgaac agggccccgg ccagcgggca gaaaagcagg
    22441 cgcggaaccc ccaccacaat gaggagcgcc atgaccagaa agaacgccgc gacgccccgg
    22501 aagccggtct gccgaaccca gtcggcggcg gccccggccg gctgcagcca ttctttaagg
    22561 ggcgtgaaac ggtaagccac gatcccggct attgcaatcg ccagaaagag cagcgccttg
    22621 accaaaagcg ccccgggccg ggccgccggt tcaggcgggc gggtctccgc cgggtccctc
    22681 tcctggacca tcgtggccat gtgtcagccc gccggggggg cctcttcctc cggcagcagg
    22741 cggtcgccgc ggaccgcgcg ggcgctgtac caccggatgc caaagcaatc gcgcagaccg
    22801 cgccacaccc ggttgcccac gccatacttc gactggccgc gcgtgcgcgg gcgatggttg
    22861 accaggattt cctcgacctg atacccctgc cggcgcagca gcgtgggaat gaaccggtgc
    22921 aggccattga aaaccgggag ctccctgacc gccgcgcggc gcagcgcgcg gaaggtgcag
    22981 ccggcgtcgg caatccggtc gcccgtaacc cagttgcgga agccgttggc aatgcgcgaa
    23041 gaaatgcgcc gcgtggcgtc gtcaagccgc tgccggcgca cgccggccac cgcggccacc
    23101 ccgggttgca gcgccgcgag catggccgga atgtcgtgcg gatcgttctg caaatcggcg
    23161 tccatcgtaa tgagaatctc gccgcgggcg cgggcgaagc cggacgcttg cgccgcgctt
    23221 tccccggagt tgacgcggtg ccgcaggatc cgcagctccg ggtgccgcgc gcggagctgc
    23281 cgcaacaccg cccaactgcc gtcggtgctg gcgtcatcca cgcagaggat ttcaaacggg
    23341 cactgcagcg agcgcaggac cgcggtcagc tcccccagca aggcggggag attatcctct
    23401 tcgttgaaga cgggaatgac gacactcagg ctgggcgatg ggagagtcat gggcataaag
    23461 ccgcgccgcc gctttacggc tggaaataat ccgcgacggc acgggagacg gtttcaattt
    23521 cttcccgggt caggcaggga tgcatcggca gcgaaagcac cgagcgacag gccgcctcgg
    23581 cccgcggcaa gtcgccccgc cggtaaccga ggtggcgata ggcgccggtt tcgtgcagcg
    23641 gttgcgggta atgcagcccg gtctcgatgc cccgcccgcg caagtggtcc gccaacgcct
    23701 cccgatccgg cgcgcgcacc acgtaaaggt ggtacaccgg ctccgtgcga gcccgggccc
    23761 ggggcgtttg gagttggggg atcccgccga gcaattgatc atagactttg gccgcggccc
    23821 gccgggcggc attccagccc tccagggagg gcaaacacac ccgcaacagc gccgcctgca
    23881 gcgtatcgag gcggctgttc aggcccacca gctcgtggtc atatttggac tcgcgcccgt
    23941 ggttggccag cattcgcatc cggcggaaaa cctccggatc ccgcgcggtg accgccccgc
    24001 cgtcgccaaa cccgcccagg ttcttggagg gaaagaagct aaacactccc gcatccccca
    24061 gggttcccgc ccggcggccg cgataacgcg cgccttgcgc ctgggcgcag tcttcaatca
    24121 gcttcagccc gtgcgcgcgg gcaatttcct gcaaagcgtc caggtcggcc agcagcccat
    24181 aaatatgcac cggaataatg gcccgcgtgc ggggggtgat cttgcggcgc acatcctccg
    24241 gatccaggca gaagccctca ggttccaggt ccgcgaagac cactttcgcg cccgccaggc
    24301 taatggcctc cgccgcggct atggccgtca gcggcgtggt gatgacttca tcgcccggcc
    24361 cgatgcccag gcccaggagc gcgaccagaa gaccggaggt ggcgcaggac acactgcaga
    24421 ccggcgcgcc gtccaggctg gccgccatct ccttctcgaa ctcccgcacg tccggcccct
    24481 ggatatagcg ttgctcgcgc agcacccgca gcgcagccgc gtccacctgc gccgcgatag
    24541 gcgcgtgcaa ccgggccagg tccacgaact tgacagtcct cgttgagtgc atccgtaagg
    24601 tgaggcagag ttgcaaagcc tcaccgtccg cgcaagcaga gaataattta acgacaaacc
    24661 ggggtgatag cctcttaatt tctcaactcc catgccatcc tccccatgcc ccacagcctt
    24721 atcaaactgt ccaattattg gacagttggc ggcgctttac tgccgaacgt tactttttcg
    24781 ctccaccaac ctcaattaag cctatcccac gatttcccac tgaatttgcg tcacttaccg
    24841 ctcctttccc gctcccttca agccgtgtac aaggggtgta caacgggagt acaagggatg
    24901 ctcgccggtt gaacaagcaa aaatcctatg tacctccggt gttcacccgt tgtacccccc
    24961 ttgtacacga tgggttggga gggggatgag atgccggtgc cggagggaaa ctggcgggtg
    25021 gagggcttgt tctaaacgct tggaaatgcg ctagattaga ttggagcagg ggatggtttt
    25081 cgtgtctgag gccggccgga gggcgggaat gagggagctt tccggattta tgggaggctg
    25141 acaagggtcg gagcggctgc tagagtcggc gtgctttgaa gagcgatcat ggattcatgc
    25201 gaatggcttg cgccaatctt gcgttctttc tctcgccgca ggggcgcaac tcgtggccgt
    25261 atcagccaga gcagcccgtt tccctgccgg aggatttttt tggggtgtgc gttgccagct
    25321 ccgaggatcc ggcgtgcgat gattacattc tcgcccggtt gcgtgagttg ggcgtgaagt
    25381 gtgtgcgggt ggattacggg tatgattcgc cgggcggttt tgccgatcgc ctggttgggc
    25441 gcctgttgag cgagggctac cgggtggcgc tgcacctggt ggcgccgcgg cgggacatgg
    25501 aggccctgga cacgccggac ggcgcggccc gctggcggga atttgtgcgc gatgcgtttg
    25561 cgcgcttcgg cggcggcgtg gaactgtttg agatcggctc gacggtgaat cgccggcgct
    25621 ggtcggggtt taccgtgccg cgatttctcc gggcgtggga gattgcctgg gcggaggcgc
    25681 gggagcgcgg actggtgctg gcggggccga atgtgacgga ttttgagccg ttctataacg
    25741 tggctatttt gcaggcgctg cggcagcgcg gttgccggcc cgggattcac acgaacaacc
    25801 tgtttgtcga gcgggcgacc gagccggagg cgtttgaccc caagattttg gggccggggt
    25861 gggccgcgcg cctcaagttt aacacggtca ggaaagcggc gctgctgggg gccatcggac
    25921 ggtgggcggg agtgccgcgg acctatagca tgcatgtgtc gtggagtttg cgccgcatcc
    25981 ggcggttgct ggtggacgtg gaagaacagc aggcggatta tgtggcgcgg tatggttgcc
    26041 tggcggcggc gtccggcggc ctgcaaaggg tgtattgggg gccgctgatt ggccagcgcg
    26101 agggcttgat tgacgatggg acaaccttct tccccgaggt gtttcacgtg acgttctacg
    26161 ggcgggccaa cgggaaggtt ggcgattacc gcattcgccc cgcgttccgc gccttccaga
    26221 cggtggtccg gtttttggcg gggacgacct tcacgcggcg aatctccgcg cgcggctcgc
    26281 tggagatcct tgaatttcaa tcggccgcgc acacgctgca cgcggtctgg tgccggaacg
    26341 gcgattgcgc cgtcgcggcg gattgttatg cggcggaggc tttgggggcc gcggaagctt
    26401 ttgatcgcac gggggcgctc ttggcggcgg cgccggtttt gtttacccag cggcccacct
    26461 atctgcgctg gaaggcggcg gccgcgattc gcctggcgcc tgcgcccggc tcaatggcgg
    26521 acctgcgcct cgcggctcgc ggggaagcgg aatatgttcc gatccggcag ccggactggc
    26581 gcgggctggc gttggcgcgg ctggccgggg ataaaattga cccggccgcc ttgctgccgg
    26641 gccggctggc gccgatggat tccgccgcgg ccagggtttt gcgcaacaag cgcaaccgcg
    26701 tctggtccgt ggcggcgccc tgggatgccc ggcgtcagat cgtggtgaag catttccggc
    26761 ccgcgaccgg gctgcgcggg tggcggcaaa ggttcaaagc cggcaaggcg cggcgcagtt
    26821 ggaatggcgc gcaggaactg ttgcggcggg ggattcccac gcccgagccc gtcgcctggc
    26881 tggagcatcc ggcgcggccg catgcgcatg agagctacta cctttgcgcc gcgtttgcag
    26941 gggggacatc ggcgcggcag gccttctacg ccgtgaacgg cggggccgcc gaattcctcg
    27001 gggagcccgt ggccggcctg ttccggcaga tcgcggagtt gctggcccgc atgcatgggc
    27061 gcggggtttt ttatcgtgat ctgtcggcgg gcaacctgct cttgcggcgg ggggccgacg
    27121 ggaacctgga gttcgcgctg attgatacgg gccgcgcgcg catcggcgtc aagggcctcg
    27181 ggattcgccc gcggctggcg gacttgatgc ggctgtgcca tccgctggcg tggcgcgggc
    27241 gcgaacagtt cctgcgcgcc tactttttgg cggcgaacat ccggtttgcg gggtggatgc
    27301 ggctggcgtt tcattactac gattggaagc atcgcctcaa gaagtggctg cgcccctggc
    27361 ggtgaacggc ggccgggagg gcgggcgcgg gcggccacgc ggcgcgatgg ggcgcatccc
    27421 agccgcgccg ggtcacggag gactggccgg cgggagggga ttcgcccgca gttcttcgat
    27481 caccgcaagc gtcttttcca cgttgagcga catcggcagg gcctcggccg tttggcgcgc
    27541 ggcggcgccg gcgcgggcgc gggcggcggg atccaggaac ggcgtaatgg ccgccgccag
    27601 cgccgcgctg tccgccgggt cttcgaccac gctgccgttg aggccggggc ggatgatttc
    27661 cgcagcgccg ttgacgcgcg agctgaccac cggcagcccg caggccatcg cctccaggca
    27721 ggcgttgctg aagggttcgt agatggccgg atgcaccagg atgtcggcgc cggcatagac
    27781 gtcctccatg cggtttgcgc tgccgaggaa gcggagccgg tccgcgacgc ccagccgcgc
    27841 ggcgagccgc tggtacggcg cggtgcggcc tttgccggcg acttccaggc gcaccgtttg
    27901 cggcagccgg gccagcgccc gcagggcaaa ttccaggccc ttgcgctcga agcccgatcc
    27961 ggcgagcagg agcacggtct gcgggcgcgc cggtggggcg gggggaggcc gaaagcgggc
    28021 gttgtcggtg ccgttgtgga tgacccgaat gcgctcggcc gggaagccgt aatgccggat
    28081 gatttcctcc ttgccgcgat gggaattggc aatgatgacg cgggtgttgg cgggggagaa
    28141 ggtccgcttt tcgatccaca gcaaggcgcg gtgaagcggg ttcaacccgg ccagcgcccg
    28201 ggcgccgccg gggcggtagc gttgccgttg ccgcaaccat tcccggtggc atccaccgcc
    28261 ggcgcggctg atatcctggc gcagggtgcg ctcgatgctc aggatcagat cgcagcccgc
    28321 cgtggggagc gcgcgtcggc tgttcaaggc gaatgataag acccgcgcaa aagccggcca
    28381 gcgaagcgcc ggcacgcgct ggagcgtgac gccggccgcg gccgcggttt catcccagcg
    28441 ggccgccagc acatgcaccg cgtggtggcg ggcctgcagt tgggtcacca ggccgtgggt
    28501 gtaccgctcc gacccgccgc cggacggaaa gaaattactt ttaatgatgc cgatgcgcac
    28561 gccgccccca gtatcaccaa aacggcggag acttccagcc aaacccaagg gcgggtccgg
    28621 aacggacggg cgggccggga ggcgggcctg gcggcgcggg gcggcgccgc gcgaacggcc
    28681 cgctgagcga tatgcggggt ttccgccggc ggctgccatt ggactgaatc aagcgggccg
    28741 gaggccggcg cgccggtttc gcaaggagga aaggcgcggg ggaacggtca cgcgggccgc
    28801 ggaaatggat tgccgcccgg cagtgattgg ttgagtatcc cgggaagcgc ttgcggttgg
    28861 gctggcgggg tgccgcccgg gtgagccgcc gtaagacggg cgcaagattt cccggcaact
    28921 tccgaggttg cgggctgaca aacaatgaag ccgcatgtgg aacagacgca aaacgccgcc
    28981 gccggccagg tctgccggat aacggcgggc cggtggcgtt gggaagtccg ggcggcgcac
    29041 gccaccccgg cgatgcaatc ggcctgggcc gcgccggagc agctcgtggc gcccccggcc
    29101 gagcgcgtcg ttcggaaccc gcccaagcga accacccagg tcttccgcgc caggttgccg
    29161 gggtcaggcg gccgggtttt catcaagcgc tacctcgccg aggcctggca aagttgcaag
    29221 agctggtttc ggctttccaa ggcggttcgc tcctttcgcc ggggcctgga gttgcagcgg
    29281 ctgggcgtcc ccacggcgcc gccggttgcg gcgggggaat accgggttgc cggctggctg
    29341 cgcgatgcct ggctgatcac agaggagatt ccggacgcgc gaacctggtt ggaatgcgac
    29401 ctggccgctc cgcggggcgc gcaccgggtt cccatgcttc gcgcgctcgc ccgctgtttt
    29461 gcgcagcttc atgaggcgaa gctttcccac tcggacgcga accgcaataa tctgctgatt
    29521 cagctggcgc ccgggcgccg cccccggttg tacctgatag acctggatgc gctggtgcgg
    29581 cgggcgtggt ttccgcgctg gcgggtggtg aaggatttgc ggcggctgca cgcgcggggt
    29641 cccgccaccc ggcgggagaa gctgtggttt atcgccgagt attgccgggc gcggaaaggc
    29701 gcgcttgagc cgcgcgaatg ggtgcggcgg gtcggccgtt ccctggcgat gccgttgacg
    29761 gtgcggcgtg tcgggccggt gcgctgggtg gtccgcccgg tgcttcaggc ggacgccctg
    29821 caggcgctgc tccaggatcc ggaggcgcgt ttgcgggacg cggcattgcg cctcaagaac
    29881 tcgcgcaatg tcaccctcgt gcgcataccg cagccggcgg ggcccggctg ggttttgcgg
    29941 cggttgaatt atggccggtt gcggcaccgg ctgcgcgatt gctttcgtcc ctcgcgcgcc
    30001 cggcgggcct ttcgcaaggg gctgtggctg gagcaggcgg gcgttgccgc ggccaggatg
    30061 ctggcggtgg gcgagcggcg gcggctgcgc tggccggttt gcgcgtatct gctgatgacg
    30121 gaaatccccg gggccatcac cctggcggag ttgctcgcgc ggcggccgcc gcaactgcgg
    30181 gaggcgctgc ggcggctggc gcaggagctg gcgctcttgc atgacgccgg tttttcccat
    30241 cgggatctca aggccaccaa cgtgttgttc gacgggcagt tgcagccgca tttgattgac
    30301 ctggacggcg tgcgccgggt gcggtggggc gcgacggcgc gggcggaggc ggatctcgag
    30361 cggctggccc gggaaacgct cgttcacccg gcggtttcaa cccggcaggc ggcctttttt
    30421 ctgaagacct actgccgccg ccggggggag gccgactggc gcggttggtg gcgggcgatt
    30481 caaaagcgcc tttccggctg aggacggttt ttccccggcg gctgtccggc ggatcttttg
    30541 cctttaccct gctcccgtgg acgggcaggc tgggcggcga ccaacgacaa cagtggatgc
    30601 cgcatttatg acctcgcgcg aacgagtcct taccgcattg aatcatcaag agccggaccg
    30661 ggtgccgata gacctctccg gtcatcgctc gtccggcata gccgccatgg cgtatgcccg
    30721 gctgcgccgg tacctgggcc tgcctccccg gccaatccgc gtctatgacc ctgtccagca
    30781 actggccatc gtggacgagg acgtgctgca gcggttccag gttgacaccc tcgagctggg
    30841 gcgcgccttt gcgctcgaag ataaatactg ggtggactgg accctgcccg acggcacgcc
    30901 ctgctgcatg ccggcctggg ccaaaccgga tcgcctcaat ggcgaatggg ttttgcgctc
    30961 ctccgtgacc ggcaaggtga tcgcgcgcat gcccgagggg gtttggtatt ttgagcaggc
    31021 gtcctttcca tttctggagc gggaagatct ggaccggctg gaagcggagt tcagcgagtg
    31081 catgtggtcg ggcatcgcct cgccgcccgg ccccatcgcc gccggccccg acggcatggc
    31141 ggtgctggcc gccggggcgc gagcgctgcg gcagcagacg gatcgcgcga ttattgggct
    31201 gtttggcggg aacctgctcg agctgggcca atggttctac cgcaacgaca actttttcat
    31261 gctgctggcg ggggaaccgg agcgcgccca ccgattcctc gacaagctca cggaaatcca
    31321 ccttcgcaac ctggagggct tcctggcggc cgtcggcgcg catattgaca tcattctctt
    31381 tggggatgac ctgggcatgc aatccggccc gcaggtgtcg ccggccatgt accgcgagta
    31441 ttttcaaccc cgggaacgcg ccatgtggca gatggtcaaa cagcgggcgc cccacctcaa
    31501 gatcatgctt cattgctgtg ggggagttcg cgagttgctg cccggcctga ttgacgccgg
    31561 gttggacgcc atcaaccccg tccagatcac ctgccgcggc atggagccgt ccgccttaaa
    31621 acgtgatttt ggcgagcgcc ttactttctg gggcggcggc tgcgatacgc gcgacgttct
    31681 catccagggc accccggagc agatccgcga gcatgtccgc cagcagatcc gcattttacg
    31741 ccccggcggc ggcttcgttt ttcagcaggt gcataacatc ctggcggatg tgccgcccgc
    31801 caatatcgtg gcgatgtatg aggccgtaat cggcgcgtaa gtgccggtgt ttccgctcgg
    31861 gcggtctttt ttggtggctg gaaggatgga atttgctatc tttaaggctt ggacaaactt
    31921 tgcttatgag tttttacgat caactcaaat ttgacgccca cggcctgatc ccggcgatta
    31981 tccaggagca gtcgagcggg cgggtgctga tgatggcctg gatgaaccgc gcctcgctgg
    32041 agcggaccct ggccaccggc aagacgcact tttggagccg ctcgcggcag aagtactgga
    32101 tgaagggcga gtccagcggc catacgcaga ccgtgaagag cgtttcattt gattgcgacg
    32161 gggacaccct gttgatccag gtggagcaga tcggcgcggc ctgccacgag ggctaccaat
    32221 cgtgcttctt ccgaacggtt cagagcgatg gcgtgaccag ccgggtcacc gaaccccggt
    32281 tgcagacgcc cgagcagatt tacggggcca agccgtaatg gaagagtttg tcccctttgc
    32341 cagccgcagc tacggcctga tgtttctctt gctggccggg gcgcggggca tggattttct
    32401 gagcacctgg gtggccacgc ccaacctggt gctggaaggc aaccccatag cccggcggct
    32461 tggctggaag tggggcatcc ccgtgaacct ggggctttgc ttcggcttcg cgttctggcc
    32521 gctgcccgcg attgtcatca gcaccaccag cgtgctggtg gcggcccgca atttccaatc
    32581 cgcctggctg atgcgcaccc tgggcgaata ccagtaccgc gagtggcacc tcgaacgcgt
    32641 gcgggaaacc cgcatgatcc tgtacctggt ttgcctgttt gggcaggtgc tgctgatcgg
    32701 cggcgtgggc gcggcggtca tctatttcag cggcggacag ctcgtgccgc tggcgatcgg
    32761 gctggggata gtcgcctacg tgctggcggt ggcattttat tcgttgctcg gcgtctggcg
    32821 cctgcgccgg acggcggacc tgacggcccg attggctggg cggaagctcg tggttgacgc
    32881 ggcggcgccc gggagaaacg ggcgggtcct ggttgccgcc gagcccctgg ggccccggcc
    32941 gtggggccag tagttcgcgg cctgatttat tatgtacgct cccacactgg atgagttttt
    33001 gaagctggcg gatcaaggga acctgatccc cgtcacgtgc cgcctgctgg cggatgtgga
    33061 aaccccgctc tcggcctatc gcaagatccg cgggcagggg gaatcgttcc tgtttgaatc
    33121 ggtggagggc ggcgagcaca tcgggcgcta ttctttcgtg ggctgcaacc cgcgggccat
    33181 catccgccag gtgggccaaa cggtggacgt gattgagaat ggcaaggtca ttcagtcctt
    33241 cacggttcag ccggcggcgg gcgcggccgg ggcgccgtct gccgagggtc tgcgcgatgg
    33301 cctggaggcg gtcgagcgcg tgatgcgggg gtatcggccc gtcgcggtgc cgggcctgcc
    33361 gcggttcacc ggcggggcga ttggcttcat tggctacgaa tttatccacg atatcgagcc
    33421 ggtggtgccc cgtccgcccc gtgatgattt gcagacgccg gtgatctatt tcctcatcgc
    33481 ggatgaactg ctggtctttg accgcgtggc gcagaccatt accgttttgg tcaacgccgt
    33541 gctcagcgat gcggccagcc ccgccgaggc ctatgagaat gccgtcggcg aaattgaacg
    33601 ccttgtttcc ctgctcgaac agcccagcga gcatcacccg gtgatcgtgc gcgaggacgt
    33661 gcccgcgctg ccgtttgaat caaaccagtc gcgcgaggat ttcctcgccc atgtccggcg
    33721 ggccaagcaa tacatcaccg ccggcgatat tatccaggtg gtgggttccc agcgtttttc
    33781 cgcgccggcc cgggcgacgg cgcttgatgt gtaccgcgcc gcccggtccg tcaatccgtc
    33841 gccctatatg ttcctgctcg aactggaggg gttcgcgctg gtgggggctt cgccggagac
    33901 gcatgtgcgc tgcgaggatc gccaggtgga gattcgcccc atcgccggca cccgccgccg
    33961 gggcgcgacg cccgaggagg accaggcgct ggaaaaagaa ctgctcgccg acccgaaaga
    34021 gcgcgcggag cacgtgatgc tggtggacct ggcgcgcaac gacctgggcc gggtgtgcga
    34081 gtatggcagc gtgcaggtcc gggacctgat gattatcgag cgctatagcc acgtcatgca
    34141 catcgtctcg caagtcgagg gccggcttgc ggcagataag acgccttatg atttgatgcg
    34201 cgccaccttc ccggcgggca ccctcagcgg cgcgcccaaa atccgcgcca tgcaagtcat
    34261 cgccgagttg gagcaaaccg cgcgcggccc ttacggcggt tgcgtgggct atttttcgtt
    34321 caatggcaac ctggattgct gcatcaccat ccgcagcgcg ctgctcaagg gtggccgggc
    34381 ttatatccag gctggcggag gctgggtgaa cgactcgacc cccgaggacg aataccggga
    34441 gacggtcaat aaatcccagg ccatgctcaa agcggtcgcc atggccgaga gcttcggcgg
    34501 ctagcgcgga ttttgcctgc gcccgggctc ggtcatagtt ccatcgagcc ttgcgttggt
    34561 ggcaaaagcc gatgctgccg ggatgaggat tttcggcttt cattttctcc tgaccgggga
    34621 ggtgtgaaag cgaatggagg cgtttctttc gccgtgcgct tcaggggttg ggtgctggcg
    34681 gacggagccg gggttgccac ctcaaaatga tctgcgcctc caccggccgg ggcggccaaa
    34741 tcccgcttgt tgcccgtgag ggcttggggc ttaatcagtg gcaatgggca aagctacgga
    34801 tatccgcgtc gtgggcgcgg cgctttactt cctgccggtt cggacccggg tggcgctgaa
    34861 gttcggcgcg gaaacgctga cggagatcac atgcgcgcgg gcatgcgtga gggtgacgga
    34921 cgaccggggg cggacggcgg aaggatgggg ggaaacaccg ttgagcgtgc agtgggtctg
    34981 gccgagcgcg ttggcgtatg cggagcggca cggcgcgttg aaagagtttt gcgcggaact
    35041 ggcggagtta tgggcgggga tgcgggcgcg tgggcacccg atggaactgg ggcatgattt
    35101 tctggagacg gcgctgccgg agcggctgca gcggttcaac cagcggcagc ggcggggcgg
    35161 ggaaccgctg ccgtggctgg ccgcgctggt atgcggttcg ccgttcgacc tggcggtgca
    35221 tgacgcctat gggcgattgc atggctgtcc gacctacgag gtgtaccatg ccgggtttat
    35281 gaaccgcgac ctgggcgatt atctgcaagc ggcggcaccc gcaggagttt cctttgcggg
    35341 ccggtatccc gcggactttc tgtgcgagcg ccgcgcggag agcctggccg cctggcacct
    35401 ggtcgggggg ctggatttac tgcaagcgag cgagcggacc ggcggcgagc cggacgatgg
    35461 ttacccggta acgctggagg attggattca gcgcgacggg ctgaagtgcc tcaaggtaaa
    35521 gctgcggggc accgatgcgg agtgggacta tcaccgttta ttccgggtgg gtgaaatagc
    35581 ctgcgggcag ggggtggact ggttgtcggc tgattttaac tgcacggtgg ccgggccgga
    35641 gtatgtgaac gggattctgg accgcttgcg ggacacttct ccgcggattt atggaatgat
    35701 tctctacgtc gagcagccgt ttccctatga cctggagcgg catcggctgg acgtgcgtag
    35761 cgtgtcggcg cgcaaaccgt tgtttttgga cgagagtgcg catgattggc ggctggtgcg
    35821 attgggacgg gagttgggct ggtcgggtgt ggcgctcaag acctgcaaga cgcaaaccgg
    35881 agccgtgctg agcgcctgct gggccaaagc gcatggcatg ggcctgatgg tgcaggacct
    35941 gaccaacccg atgctggcac agataccgca cgctttgctg gcggcgcata cgggaacgat
    36001 catggggatt gaaaccaacg cgatgcagtt ttacccggaa gcctcccgcc cggaggcgga
    36061 agtgcatccg gggctttacc ggcgccggca ggggcgaatc gagctttcca gcgtgcgcgg
    36121 gcccgggttt ggttatcggt tggcagaaat ccggcgggta ttgccgccgc cggccgtcga
    36181 atttttcgac tgagaccttt cgtttttcag acataaaacg gcgcgaaacc gcgctgaaaa
    36241 cgcggcgcgc gccggaaggg agattggccg cttcatggca ttggtcctgc tgttttcgag
    36301 gggaagttca gtcacaatat tctaagatat gaagcgtccg ctgcgcctcc tgcatctgga
    36361 agacgacccg gattactccg atttggtgcg aattatgctg accaaggagg ggtatgaggt
    36421 cgaggtagtc ctggccgaag gccgtgcggc ctttgaaaag gctttggccc cggagaggtt
    36481 tgatctcatt ctggcggatt atttgttgcc gtcgtacaac ggcctggcgg cgctgcgggc
    36541 ggcgcaaaag aaatgtcccg aaaccccgtt tttgctggtt tccgggacca ttggtgaata
    36601 cgcggccatc gagagcctca aggccggcgc gacggactat gtgcttaaga actggccgga
    36661 gcatttggtt ccggctgtgc ggcgcgccat tcaggaggcg gaagagcggc gtcagcgacg
    36721 gcgcgccgaa gccgaactgg ttcgtcggga gcgacgcttt cgcgcgatca ccgagaactc
    36781 gctggacatt ctcaccgttt tggatcagga gggtcatttc attcacaaca gcccgtccat
    36841 caagcgcgtg ctgggatatg agccccgggc attgatagga aagagcgcat ttgcgcttat
    36901 tcatcccgag gatttgccgc gggtattgca ggccttcgag gccatcctga aaacccggga
    36961 gcgcccgttc caggccgagt accggtttcg gcaccaggat gggtcgtggc gctttctgga
    37021 aggcgtcggc cagaaccggc tgcaggattc cgcaatcgcc ggggtcgtgg ttaacgcacg
    37081 ggatattacc gaccgcaagc aagccgaaga ggccctgcgc cgcagcgaag agcgctaccg
    37141 cctgatcttc gacagcaacc cgaagccgac gatggtgtat gaccatctca ccctggcgat
    37201 tttggaggcc aacaaagccg ccgtccaaca ctacggctat tccgccgagg aatttaaaaa
    37261 gctgacgctg cgggacattc gtccgcccga agatgtgccg gccatgatcg agtatatgca
    37321 ccggatcgcg aaggccgaac cgaataacca ggtgcgctac gccgggatat ggcggcaccg
    37381 gcggaagaac ggggagatca tggacgtgga aatcaagtgg tgcccgatcg cgtttggcga
    37441 ccggatggtt tcgctggcca tgatcaccga tatcaccgaa cggaagcgcg tggagttgcg
    37501 caacgcggcg tttgccaagc tgggccaaaa cctgaattcg gcatcctcgc cggccgaggc
    37561 ggcccggatc atccggtcgg ccgcggacga tctgtttcgg tgggacgcgt ttacgctgga
    37621 cttgtatagc gcggaagagg gcaggatata ccccctcctg aacgtggata ccaacgctcg
    37681 cggccagcgg tttgaggacc cgggaaccgg ccagcccaag gaattgagcg gaatggcgcg
    37741 gcgcgtggtg acgaaaggcg cggaattgat cttgcgggag gagccgttcg tcatccccga
    37801 ggatgtgtcg ccgatcggcg accccgcgcg gccttcggct tcgttaatgc tggtgcccat
    37861 ccgcaatcga acccaggtga ttggcctgct ctcgattcag agttatacgc ccaaagccta
    37921 ccaaccggcg gacctcgcca cattgcaggc gctggcggat cattgcggcg gagccctgga
    37981 acgaattcgg gcggagcagg cgctgcggga cagcgaacaa cgcttccgcg agcttttcga
    38041 aggctcgccg gacgccgtgt ttgtagagga cgtgcatggg ggcgtgctgg atgtcaaccc
    38101 ggcggcatgc cggctgcatg gcatgacgcg ggaggatttg atgggccgga acatatccga
    38161 actggtgccg cccggagagc aggcggcggc ggcgcagaag tttcaggagc tggtggaggg
    38221 ccggttggcg cagatcgaag gcgccagcta cacccgcgat ggccgaatcg tgccggtgga
    38281 gattcgcgcc agccgcatca cttacgccaa tcagcccgcc gtgctgttgc acgtgcgcga
    38341 tattgcagac cggaagcgcg ccgaggcggc gctgcgcagt tcggagatgc tgtttcattc
    38401 ggtatgggaa aactcggcgg acgggatgcg cctgaccgac gagcatggca acatcgttgc
    38461 ggtcaacgag gccttctgca agttggtggg cctgcgccgg gaagagctgg agggcaaacc
    38521 actaaccgtc acggaagccg gtcccgagcc gccggagcag cggctgatgg aatactgcca
    38581 gcgcttccgc gagggggcca cggaaaagca aaaggaacgg caactgaccc tgcgcaacgg
    38641 gcaggaggtg atcctggagg aaaccaactc acgggtggaa ttgcgcggcc ggccgccctt
    38701 gcagttgggc atattccggg atgtgatggc gcagaaacgg ctggaggaac agttgcggca
    38761 gtcgcaaaag atggaagcca tcggccagct tgccggcggc gtggcccacg atttcaacaa
    38821 tatccttacg gtcattcacg gccatgcctc gctgctgatg gcgaacggcg ggctggcccc
    38881 cgttcacgcg cggtcggcgc aacagatcgg ccaggctgcg gagcgggccg cctccctgac
    38941 ccgccaactg ctggcgttca gccgccggca ggtgatgcag ccgcggcagt tggatatgaa
    39001 tgaagtggtc agcaacatga ccaagatgct ggggcgcatc ctcggagaag acatcaccct
    39061 gcaactgaat tattggccgc agccggcgct ggtgcaggct gacaatggca tgatggagca
    39121 ggtgcttttg aacctggcgg tgaacgcgcg cgacgccatg cccaaaggcg gcttgctggc
    39181 cattaaagtc gcggaactcg aagtggaggc gccgccgggg cgggcgcact cggaggcgcg
    39241 agccggccgg tttgtgtgcc tgagcatcac ggataatggc tgcggcatcc cttcggagaa
    39301 tttgtggcgc atctttgatc cgttctttac caccaagagc gtggggaaag gcacggggct
    39361 ggggttggcc accgtctatg gcattgtcaa ccagcaccgg ggctgggtgg aagtggaaag
    39421 cgaactgaag cgcggcacgg tttttcgggt gtttctgccg cgaagcacgc aaccggcaaa
    39481 ggcgccggat gaaccgtcgg cgcccgcaat cgttcgcgga ggccaggaga ccatcctggt
    39541 ggtcgaggat gagacgcccg tgcgcgagtt ggtttgcgat ctgctgcggc ggcacggcta
    39601 ccacgttctg ccggcggaat ccagcgccca ggcgctccag gtttggcagg gcagcaagga
    39661 ccggattgat ttggtgctga ccgacctggt catgcccgac cagatgaacg ggcgcgaact
    39721 ggttgaaaaa ctttgggttg aacgcccccg gctcaaggtg cttttcacca gcggctacag
    39781 ccaggaagtt gtcggcaagg attttgtcct gcggcgcggt ttgaattatc tgcaaaaacc
    39841 gtatccgccg cataaacttg cccgggccgt gcgggattgc ctggatgcgg tgaattaggg
    39901 cgcctcccac agccaaagtc gtgccaagtt cttgcttgtg tcggcggccg gggggaatag
    39961 aatctcacca tgccacaagg cattcattta tcgcagcgtt tggtgcaatc gcaggtgttg
    40021 gcgccgcaat tgcagcagtc gcttgccctc ttgcaggcgc ccgtgctgga tctcaaggcg
    40081 ctggtcgagc aggaattgca gcaaaacccg gtcttggaag aggtttcgcc ggccgaagct
    40141 gaatccgcgg agacggacga tcgggagctt gaagcctccg gcgaagccgg cgaacccggt
    40201 gagttgtcgg cggactcgga tttcgatgcc ggccccggcg aggacacaga ggggccggtg
    40261 gatgcgttcg aagcggaatt cgagcggttg gcgcaactcg atcaggaatg gcgcgaccat
    40321 tttgcccaga ccaatctccc ccttcgcacc tccgccgagg ccgaagagaa gcggcagttc
    40381 atgtttgatt cgctggtggc agagacctcg ctgcaggagg ttctgcagga gcaggtgcgt
    40441 gattccgcgc tgtcgccgga gcagttgccg attgcggaaa tgctcatcgg caacattgat
    40501 gactatggct atctgaaagc ctccctggaa gaactggccg gggccaccgg gttgatggtg
    40561 gaccagatcg tcgaggtgct gaaagtgatt caaaccttcg agccgccggg ggtgggcgcg
    40621 cgcgatctgc aggaatgcct gcggctgcaa ttggagcgcc agggccggca gaacaccctg
    40681 gaatatcgca tcatcaacga attcatggag gccctcggca aacgccgcat ccccgagatt
    40741 gcccgcggca ccgggcaatc ggtggacgag gtgcaggcgg cgctggcgcg cattgccaag
    40801 cttgagccgc gcccggggcg ggcttatttg ccggatgacg accactacat tctgccggaa
    40861 gtattcgtgc gcaaatcgga cgacgattat caggtcacga ccaataatga gcatgtcccg
    40921 cacctgcgca tcagcaatac ctacaaggat ttaatggcgc agcggggaaa tcccgccgaa
    40981 gtgcggaatt acatccgcga gaaaatccgc gccggaaagt ttctgatcaa gagcctgcag
    41041 caacggcagc agaccattct caacatcgcg cgcgagatag tgaatcggca gcgcgatttc
    41101 atggccaaag gcgtgatcgg gctcaaaccg ctcacgatgg tgcaggtggc gcaggtggtc
    41161 ggcgtccacg aaaccaccgt cagccgggcg gtctccggca aatacatgga gacgccgcag
    41221 gggatcttcg agatgaaata ctttttcacc gccggcatcc agaccgccag cggtgatggc
    41281 atgtccaaca ccagcgtgaa ggacatgatc gcggacatct tcaaagccga ggattccgcc
    41341 aagccccttt cagatcagga ggtggttcgg atgctcaagg aaaaaggcat tgtgattgcg
    41401 cgccgcaccg tggccaagta ccgcaccgag ctgaacattc tgccttccaa tctgcgcaag
    41461 gtttactgac ccgcgcttgc gggttggccg ctcgtccgcc ccgccggccg aattgcgggc
    41521 gcgggccggc ggcgccggcg cctgcctttc ctcaggtgga cagccagaac gccagctcgt
    41581 tgacctgctc cggggcgccc agtatcagca gttggtcccc cgcgtgcagc gcttccgatc
    41641 gtcccgggct caccaccagt tgctggtcgc gttcgatgcc cacaatttga atgccgaaca
    41701 gcgccttgag cggcagttcg cccagcgcct tgccgatgtg cctcgagacc gccggcaccg
    41761 tcaaccgctg gaggcttaaa tcagacagtt gcggctcccg gtgggcggct tcggctccct
    41821 cggcttcctg gcttagccag cggtccgccc gccgcaaatc ctcattccgg cccagcagca
    41881 gcactttgtc attcggatac aggatggtgg cggcgccggg gttgggaatc acgatcccct
    41941 ggcgttcgat gctggcaatg gtgcaggaaa acaaggggcg cagcggcaat tccgaaatcg
    42001 cccgcgccac cgcccgcgta tgttcgccga tgacgctttc ggccaggttg agctttccct
    42061 cgccgtttcc cttcggccag cccgcccagg acccgttcgc ttccgcaaag ggggaatcca
    42121 ccaactgcgt ccgcagctcc atttcaaagc ggttgtgcaa acgaatcagt ttgcgccaga
    42181 acaccgcccc caccaacagg aacgccgcca tcaatcccgc cacctcccac gccggcagcg
    42241 tgccgtaagg aaacagcgcc gccagccaaa ccaggatcgc caccagggcc gcgcccttga
    42301 ccaggcgttc aaaaaagaac tgcgcttccg cgcgcttcga ccggcccgcg gccgccgcct
    42361 ccgcgcagag cagggccacg gtttccacgt tccgccagag cgccagcagc ggcccaagca
    42421 acagcagccc aaatccaaac cagcaaactc ccggcaaacc gccggcggcc ggccagtccg
    42481 ttcccagcca tttctccgcc agccggtaca gcggtttggc gaaaatcacc aggccggaca
    42541 caaagaggat taaacaggtc atctggacca actgcggcgc cgccagcttc cacaacaggc
    42601 tggattgccg ccggtgcttc aaccgctcga tccattcatg gtagaacccg attccctgcc
    42661 gcaaaaactc cggttgcttt tgctcaagat attcactcaa cgcctccgat ttccggatca
    42721 gcaccggtgt ggtcagcgcg gtcagcaggc taaccccgat cgccagccca taaaacgacg
    42781 ccggcacgag gccgccctgc acgccggtca gcgcgatgat cagggaaaac tcgccgatgg
    42841 gggtcaggca catcgccgcc cgggaagctt cctttatcgg ttgccccgcc agcaacagcg
    42901 cggccgtcgc cgccaccgcg cgccaaacca ccgccaatcc gaacacgccc agcagcagcg
    42961 gccatgtggt tgccagcgcc ttgaattcga acaacatccc catcgctaca aaaaacaccg
    43021 gcgccaatac ttcgcacatc ccctccaaca cccgctccac cggcccgatc cgatccgtgc
    43081 tcgacatgat cgagcccagc aagaacgctc ccagcgccgc cgaaaatccg gcccgggccg
    43141 acaacagcgc catcgccagc agcagcccca cggccaccag cgaacgaacc tcggacgcga
    43201 tcccccgccc caggcgcttc agcactgacg gcaccagcag caaggctcca atcgccatcg
    43261 tcaccatcac cgccttgagc tgaataatgc ttccgaacac cagcagcgcc ccggtttggc
    43321 ccgcttgcgc gaccgacgcc agcaccgtca gcatccccac cgcaacgaga tcgtccagcg
    43381 tcgtcaccgt cagcgccgtc tgtccgaacc cgctatgaac tgcgtttgcc tgccgcaggc
    43441 tcttgctcag cacggcggtg ctcgaaacca tcagcattcc cgccagcacc aggctgtgca
    43501 tggccggcca tcccagggtc gcccccagcg cccggcagcc caccagcacc agcgcggcca
    43561 ttcctgccgt tgccagtatc agcggcaacc cgacccgctt caaccgctgc agccgcaccc
    43621 tttgcccgat gctaaagatc aaaaatacca gcccgatctg cgccagtgat tgaatcctcc
    43681 ctgaatccga caccagcgcg tgggggcccg caagcggccc aatcagaatc cccgcggtga
    43741 tatatcccac cacccccggc acgcccatcc gctggcaaac ccaggcggcg gcggccgcta
    43801 tgagcatcac caacgccagg tcctgaataa aatcgaagcc aatcacgcca tttcaacctt
    43861 tccaggcctc cgcctgggcc gcccgcccgg ttcccctcgc gcgcggaccg cggggcaggg
    43921 cgtctgccgc tatcggccgt tcgtcaggct ccacaaccga tgtccgagcc ttttttgcgt
    43981 tcgcagcccc agaacgcccc gccgatcacg cgcaacggcc cctcgtcgtc cgggcgctcg
    44041 tccgccaatg aaaatatccc gttcaggaat tgcagttccg aggaaaactt catcggggcg
    44101 ccttcccgtt cctgcagcag ccgaaggaat gtctcgtagc gctgggctac gtaacggcaa
    44161 taaacctcgc tgccgcagac gcgctcccgc gggggcgcgc cgggctcggt cagcaagtcc
    44221 cggacaatct ccaggaagat cgggacatca atcggttttt cgatgagcgc ttccactccg
    44281 gccgcccgcg cccgctcttg ctggccgtgt tccgccgtga cgatcacggt cggcaccagc
    44341 gggtccacct cgctcatctt ctgaaacacc tcccacccgt cgtccgcccc caggttcaca
    44401 tccagcacca cgaggtcaac ccggccgggt tggaaatggc ccaacgcctc ggcgcggtcg
    44461 cgcgccacca gcacttcata cccctcggcc gccagcaatt ccgtcatggc ctcccgcacc
    44521 gagagatcat catccactac caataatctt cgtttcatac gcattcagag tttggttcat
    44581 cggaataatg ccacatgccc ggctttgcgt tgcgcccgct cccgcagact ctctgacaat
    44641 aacgtctcga ttgcgtccag cagcaccggc gtcgcggcct tatccgtcaa caccatatcg
    44701 gccatgaatt gcgtgggatc gtccaattcg tcttcccttt tgcacaacag cagcaacggc
    44761 aagaacgggt caacatccgt caaagcctgc agggttcggc tggcgtgccc ggcgcgcccg
    44821 gatgcgttgg gcgcggtggt gaaatccacc acgaccaggt tcaccggacg ggcggccagc
    44881 agcgccgccg ccgggcggcc cgtcttgccg cgcgtcacgc cgtatccttc ccgctccagc
    44941 agggccgtga gcgcgtcctg ccgggccgga tcgtcactaa taacgagaag gttctttttc
    45001 atggccgcaa cattacggtg gttgttgcct tttctttagc ggatgccgtg ccacccggcg
    45061 ccggggctgt tttccgccat tttccgctcc tcccgcgcct ccccacctgc cattccgccc
    45121 gcccgggcgg ttcatttggt ttcggcgccg gtttaatttg tttcaaccgc accggacccc
    45181 cggtcccggc acccccccgc cgacgccgtt gccagccgcg tcgtgctccg ctgaaagcga
    45241 aaccctcgct ggctcatgcc gcgcaggatt ttggctcgca cccgggccga cagggcttcc
    45301 ggcggataga cccccgccag gtgccccggg aagtgttcta caaacaccgc cgcgcattga
    45361 gccgtggcaa aggcaatcgg agttcccgct atccgggctc gccggaggca ggcaaggctt
    45421 ggaaacacac agcaacgctg ccgttccacc tgccgggctc ctttccgtcc gctcaccacc
    45481 acgcagaccg cgaaccgcgc gttctgcagc ttgccttgcc gcatcagttc ggatacctcc
    45541 tccggcgttg ccgtgtcggg aaaccgctcg ttcacccgtt cccctgacgc ccgcagtttc
    45601 ccctgccgat accagcgccg cagccgatcc acctcgttgc ccccgatttt tacatccagg
    45661 ttgttcatct cgataaagcg cggcagcgtg gccgcctcat cctgcgccgc cagcaccacc
    45721 cgcgacttgc caatgggccg cggaaatcga aaccactccg cctcactgaa acgccgggcc
    45781 agccgaaacc gcccgtcgcg ataaatccgc ggccgcgaag tcgcctcgtc atgcgccacc
    45841 tccgccgacc aggtggacac gggcgcgtcg ctctcgagat cctcaaacag ccggatgcgg
    45901 acatggtcca cctcgtccag cgtatccgcg caggccgcca ccagcaggtt ggtcaggccc
    45961 ggagccacgc ccgcgttgat caaccccagc cgcccgcggc gggcaaaatc gtcctcgaaa
    46021 cgaagctgct cggccttgaa cggattgcgc gacagatgcg atgccaggtc caggtagtga
    46081 acccgcagcc gcagcgccgc ccgcatgatg gtttcgttaa agaccgccgg cgccgcgttg
    46141 atcaggagct ggcatccgcg cgcggcccgc accaccgagc gcaggctccg cccattggcc
    46201 tggcggcatt caaaagcgtg tcccgcaaac cggcgcgccc gctcggggtc gcggtcgccg
    46261 caccagaccg tgtgcccccg ccgccgcagc aattgcgcca gcaatccccc ggtcgctccc
    46321 gttcccagga tgaacacgcg catcccgccg ccctttcgca ttccgtttac ccccggcggc
    46381 ccggcatccc tgtgtgcgcc ttccggcgct ggctccgcac ccgccgaacc agtcgcgccg
    46441 tctttcgccg ccgcaactgc cgctccatct tatccgccac aatatccagc gccgcgaggg
    46501 ggtcatgggc ggtgtgctcg gccaccaggt cgtttcccgg cagttcgtaa tgcacccgca
    46561 cccgaaactg cgcgggagac ggattgccgc gcaccttgtg caactgcacg cgcgcacgca
    46621 tggcccgcgg cgcgtaccgc cgggcccggc cgatcttccg gctgaccgcg gcgcgcaact
    46681 ggtcggtcag ctccacgcca tccgcccgga tgagaatgtt ctgcgagtcc tcctgccagg
    46741 gactgaccgg tatcctgctg gccgcccgga cgcggcgtgg ctgcaacctg ctcacattgg
    46801 tcttcataag taatgccttt gcgttgggcg cggcttattt tttccgcgcc gttacctgcc
    46861 ggtctaagcg gacttcgtgc cagcccgttt tcccacacgg aaatcaagca ttctcagcca
    46921 cttcagcccc gtgaacgcca cttgcggcgg aagcttttgt tccgcgcacg gtctgttttg
    46981 tcccgtctgc ggcgtccttc cggggaaaaa aagcggcctg ctccgccatt tctcaccggc
    47041 tccgccccgt aagataagcc tgtttgccgg caaaagccat cgcaaatttc cgcctcaacc
    47101 acgtttgccc gggtctttgg ttttggcgct cataagccgg ggcggttaag gctgcgggtg
    47161 ggacgggatt aagtgcttcg gagcgattgt ctggccgtgc gcccaaggct tgggctgctg
    47221 ggctcagata ggactggtta gctttgctca gaagaagatc gtcgccgccc ttttgcccga
    47281 tccttcgggc cagtttgcgc tggccgtgca aaggatcggc agtgatgagc ttgccattgc
    47341 agaaactcag ccgccgagaa caaaagcccc tatggccgca ggcaacccgg
//
LOCUS       DAOPQG010000010        45029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1073_length_45029_cov_16.504202, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000010 DAOPQG010000000
VERSION     DAOPQG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 45029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45029
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_45029_cov_16.504202"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..833)
                     /locus_tag="PKI07_02060"
     CDS             complement(192..833)
                     /locus_tag="PKI07_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOC49865.1"
                     /translation="MKSLLKQTLPALLLVALLGNSAWAQGRIATVDLRKLFDNYWKTK
                     QADAALKERAADIEKDHQTMIEDWKKAKDDYQALLADASNQTLSVEERDKRKKSAEEK
                     FKQIKDSEEAIAQYERQARTSLDEQRKRMRDKLVEEIRAVVKAKAVSAGYGTVFDTAA
                     ESANGTPILLYASGENDLTDALLAQLNVGAPAEAAKTDEKTANKQDEKKKDKK"
     gene            complement(952..3381)
                     /gene="bamA"
                     /locus_tag="PKI07_02065"
     CDS             complement(952..3381)
                     /gene="bamA"
                     /locus_tag="PKI07_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOC49866.1"
                     /translation="MKLLLRLCVPLLLLAWLPAGWGQPAAAKIQAIRIQHIGPPATSD
                     ELVRAQLRTKVGDSYLPAAVDDDVRNLYATGFFYNVQVTADTTAEGMTLTYKVQGRPR
                     LTEIKFQGNTRFKDAQLLKKLSSKAGEPLDLRKLFTDTQELQKLYQKKGYSRTEAKYE
                     YHLDEPSGLATATFLITESPKIKIVEVDFVGAKAFPLKKLRKVIKTRKHWMFSWLTRR
                     GVFKEEQFEEDKEKLAEFYRKAGYIDFAIKDVQFVNPTPRTLIVRFTLFEGNQYKVGA
                     IKFTGNKLFSTPEIVAGLQAGHKRSRSKAALGPNGLPMDVGGVFTAGGLAKDVEAIED
                     FYGAKGYIDVQESSRNLRVVKIPNTETGTMDLEFQIEEGQPSYIEKIEIRGNTRTKDK
                     VIRRELAVAPGELFDMVRVKLSKSRLEGLSYFDRVEARPEPTDVPNRKNLVFDVEDKR
                     TGNVMLGAGFSSVDALVGYVELYEGNFNLSHPFQPPWFRGGGQKLRLRAMVGTRRQDY
                     QVTFIEPWFLDRKLQLSVDLYYHEYSFLSPKDLYDETRVGGRVGLERALGSEFLRGGV
                     NYTLENVNIDLTHKAVTEGPRANVPDDIRDARGDHLLSRVGTSLAYDTRNSVRLPNKG
                     QRTELTAQITTGSDTFYKLELTSAWYFRGLFPGHVLELGGRSGVAEALGGTDDVPFFE
                     RYYLGGLYNLRGFKYRYISPRQKPELRGNEPIGGDTYWFGSAEYSLPIFEQDRGIGVR
                     FAIFYDIGSVDRRSYRLNLSDFSDNWGLGLRLNLPIGPLRLDYGFPIHHDKYNSGSGK
                     FQFGVGWERMF"
     gene            complement(3392..4855)
                     /gene="dnaB"
                     /locus_tag="PKI07_02070"
     CDS             complement(3392..4855)
                     /gene="dnaB"
                     /locus_tag="PKI07_02070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOC49867.1"
                     /translation="MTNANTEGNSGAPKDLHRAPRRSSSAASRARVDRLPPHSPEAEQ
                     GVLGCILLSPNECLGECIGKFKRGAEAFYDLRHQTIFSALIEMYDARQAIDLITLQQH
                     LKNRQLLEEVGGLAYLGSLSDAVPSAANLAYYLDIVQEKYLLRKMVQVCTETVNRVYE
                     HEGEVDQLMDEIERNILRVSESRVQSQTVKIKELVHQAINIIEKFHEGKGQLSGLGTG
                     FTDLDRITSGLHGGEMIVIAARPSMGKTSLAMNIAEHVALEQRQPVGVFSLEMTAESL
                     VLRMLCSRARVNLRSIREGFLAERDFPKLTAAAGKLSNAPLFIEDSSALSILQLRAQA
                     RRLAQQYGIKLFVVDYLQLLHSTSRRAENRQQEIADISSGLKALAKELMVPVVVLSQL
                     NREIEREKGRPPRLSDLRESGAIEQDADVVGLLYKPKASGDDEDGGGAVEEDAVPVNL
                     LIAKQRNGPTGDVNLMFLKSYTRFESAAKVSDEDVPN"
     gene            complement(5150..5638)
                     /locus_tag="PKI07_02075"
     CDS             complement(5150..5638)
                     /locus_tag="PKI07_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOC49868.1"
                     /translation="MSELFWFVAHTRPRREKKLAEHCRRQQLAVVLPCYRSAHKYRGK
                     TVVFDKPLFPGYVFLQLEPEQKAAVRQNDHVANLLEVYDQATFQRQLQAILLALEAKV
                     GVRLAPAIGAGMRVRIKTGPLQGIEGWVERRSGMSTVLLRLDFINQAAAVKLDADALE
                     LI"
     gene            complement(5739..7865)
                     /locus_tag="PKI07_02080"
     CDS             complement(5739..7865)
                     /locus_tag="PKI07_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC49869.1"
                     /translation="MSKRTKTGAAAAAESGVGDASGWAWLRRPGVLCLLLALATLAVF
                     LPVAWHGFVNYDDADYVTENAQVQGGLKWASIVWAFTTGHASNWHPLTWISHMADCQL
                     FGLQAGGHHLVSVGLHIANALLLFGLLRRMTGALWRSALVAALFALHPLHVESVAWAS
                     ERKDVLSTLFFLLTLWAYAAYAEGRRPEARPGRARRPVANYLLALGCFALGLMSKPML
                     VTLPFVLLLLDYWPLQRWPRRAPAAQPDTAPGRPRPGLFFLLLEKAPFFALAAASSIV
                     TYFVQRHGGAVSTSLTFGERVTNALISYARYIGKTFCPLDLSILYPHPGRWPLGAVAG
                     AAVLLLAISVVVALRARKQPYLAVGWLWFCGTLVPVIGLVQVGIQSMADRYTYVPLIG
                     GFIMLVWGVDALVPARPGRGKALALGAGLGLAACALLAARQVRFWRNSEELFGHAVRV
                     TRDNYLAHNNLGYYLNGQGRTAEAVEHYRRALQIKPTYEDALNNLGYALAAQRQFAEA
                     ISLYEAALRSRPKHIEVHNNLGNALSEVGRIDEAIQHYSFVLQQKPGHADAHNNLGIA
                     LAMQGKLDEAIPHFHEAIRAKTNYASAHSNLGNALAAQHKLDEAIQEYQIALRLKPQD
                     PQAHNNLGNVLAEQGKIPEAIAQYREALRLRADNPEAHFNLALGLLRQGHREEAAEHL
                     RAALRLRPDYPDARRHLEALAPAGQN"
     gene            8150..10447
                     /locus_tag="PKI07_02085"
     CDS             8150..10447
                     /locus_tag="PKI07_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOC49870.1"
                     /translation="MVGIDFIQDLGLVMLVAATAGWLCRRLGLPLILGYITAGILIGP
                     HMPFFVMVDDTNRIHALAQVGLVFLIFQIGQGLQLQRLKSMGLPLVLATFIIAIFVFN
                     GSRLLAYLLGWPPVYGLVLAGMLMVSSTAIIGKTLRENNALHSIFGQTALTVTALDDL
                     VAVVMLTLLSVIQTSAAVAPGALLETIFRLAAVMATMLIVALLAVPPLIKRLNRNTSG
                     ELRTLFIVGLLLAMALLSTKAGFSAAIGAFLLGSVVASTGHKAQVEQSLGGLCEVFGA
                     LFFVAVGMLFELKLLAGVWPLVLLVFAFALLWRSFASSLALLVVGQQTGDAVRAGICL
                     TPIGEFSLIIALAAVQARIVPDWFYALAIGLCLLSTLTTPIIIRHPGRFSDGFERWQP
                     EFVRKAVEFYHDWVESFRHRQRSSLFWRLTGPRWLQLAGEVLFISGLLIFAQPGYRLI
                     AQWLSPDWPAPIGLPALFWLGFLLLLLVPLVALWRTVEVISMICAEAATRGHRHRDKL
                     QPAFEKLLHGAAYVLILAWVSIFVPYESLPRKSLFAFLAVAVALTLLFWRKLVRWHSR
                     FQIDLRAHLQGSAFAAPGVSRANWQQHSERWHLRAREHVVGEQTRAAGKAIHDLPLRQ
                     MFSANIVGIERQGYHLPNPEPNTVLYPNDKLLLLGAEKDLRQAERWLSALEPQPASGN
                     GREPSFADLCLEPLLVPASSRHIGKSLGELKLTQQLGIQVVGLEREDDTRLTVGKFDT
                     LKAGDRLLVLGTQKQITDMAFWLAT"
     gene            10495..10908
                     /gene="rnk"
                     /locus_tag="PKI07_02090"
     CDS             10495..10908
                     /gene="rnk"
                     /locus_tag="PKI07_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOC49871.1"
                     /translation="MTAPAKRTIYITNADMIRLRSLVASPKHGRDDLDSLRSELDHAR
                     VVEPADLPADVITMNSQAQLRDLESKEVMTFTLVFPEKANPEQDRISVLAPIGTAMLG
                     QREGDEFDWEVPAGRVRLKVLKVLYQPEAAKHYHL"
     gene            11409..12164
                     /locus_tag="PKI07_02095"
     CDS             11409..12164
                     /locus_tag="PKI07_02095"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOC49872.1"
                     /translation="MKSSRQELQELRVEAKYLLSHDQARQLRPLLRRRLALDAHGAAH
                     PDAGYDVHSVYLDSNRLHTYWAALKKEKDRAKLRIRFYDDDHAAPAYVEVKTREGGQT
                     IKRRCPVRRDQLGAVIAGRVPGPLAGETAPEGHSRALQHFIGKLLKLDAGPKLHIAYR
                     REAYVSKNSNAVRVTLDRDVRSEPVTALRLTTAMRNPQPLCGNNVVLEVKVTGPMPPW
                     LRQLVAGLSSPHRRFAKYVFSVQQHGLHRQSQD"
     gene            complement(12242..12316)
                     /locus_tag="PKI07_02100"
     tRNA            complement(12242..12316)
                     /locus_tag="PKI07_02100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12282..12284),aa:Val,seq:gac)
     gene            complement(12423..13427)
                     /locus_tag="PKI07_02105"
     CDS             complement(12423..13427)
                     /locus_tag="PKI07_02105"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOC49873.1"
                     /translation="MKIAVVGCGAVGSYYGAQLARAGHAVHFLLRSDYEAVRRQGVRI
                     QSPEGQFQVHPHCARRPEEIGPADLVLIGLKTTANGQFPALLPPLVGPESAVLTLQNG
                     LGNEEQLARLFPAAQILGGLCFVCLNRIAPGVIRHIGFGQVVLGEFQRPPAPRTRAIA
                     ARFAEAGVPCTVTDDLARTHWEKLVWNIPFNGLGVAGAAGPEVLHQLAGSEGSAEDAL
                     PPGSPPGGCLTTDILLADARWSRLVRELMREVIAAARALGHAVPDDFAGPLIERTRAM
                     GAYKASTLIDFERGQPLELQSLFQEPLRQALRAGVAVPRLAALCQVLERLADRQPAAG
                     "
     gene            complement(13485..13772)
                     /locus_tag="PKI07_02110"
     CDS             complement(13485..13772)
                     /locus_tag="PKI07_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOC49874.1"
                     /translation="MAKALSKSQIAAAIAEKNDISKKQAVQILESICELAYKQAKNTF
                     TFPGLGKLVLVNRAARTGRNPATGATIQIPAKRVVKFRVSKAAKDAILGAK"
     gene            complement(13970..14437)
                     /locus_tag="PKI07_02115"
     CDS             complement(13970..14437)
                     /locus_tag="PKI07_02115"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOC49875.1"
                     /translation="MHEKFMRAAIRLSVQKMRQGQGGPFGAVVVRRGKIVGRGWNRVT
                     TANDPTAHAEIAAIREACRRLRSFQLDECDLYTSCEPCPMCLAAIYWARLRQVFYGNT
                     RRDAARIGFDDDFIYREVALPIGKRQRRLRQLLRPEALAAFREWERKPDKVCY"
     gene            complement(14464..15321)
                     /locus_tag="PKI07_02120"
     CDS             complement(14464..15321)
                     /locus_tag="PKI07_02120"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC49876.1"
                     /translation="MTPYHQLVSDYARLLKQGRALPLGTFPPAARPEIAPHAPKALFF
                     APHPDDECIVGALAVRLMRQARMNLINVAVTLGSKKERQQERLRELQAACHYLGFTLV
                     TTAPNGLERITPKTREQDPQYWAACVGVIKNILEQHQPKVVLCPHDRDWNGTHIGTHH
                     LVVDALKQMPASYACYLVETEFWGAMTDPNLMVEVSAEDLADMIAATSFHVGEVKRNP
                     YHLLLPPWMMDNVRRGGEVVGGQGGAAPDYTFAVLYRLRKWSQGQAVRFYEGGKQAPL
                     SLNIAELFA"
     gene            complement(15514..16917)
                     /locus_tag="PKI07_02125"
     CDS             complement(15514..16917)
                     /locus_tag="PKI07_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOC49877.1"
                     /translation="MNTNNGLPLIAPRVTPVLDPAFRPAVLANRAFRALARATAGSGM
                     VRLALEQTDGTVSHYSSEILPDSHPQAAGNFAYLERIAKFLLWSRGGFRIHVEGPAAL
                     AAKLAAHYRETPTGKFDSHLVGERMFDQPIEVVHAQTLPPERHGATSLGRHLDGCRIG
                     FDLGGSDRKVAAVIDGKVVFSEETVWDPYFQPDPQYHYDGIMDSLKKAAAHLPRVDAI
                     GGSAAGVYVNNRVKVASLFRGVPADVFTRRVRDLFLEIRKAWNQIPLEVVNDGEVTAL
                     AGSMSLGQNRILGISLGTSTAGGYVNAEGNITSWLNELAFVPVDLNPAAPTDEWSGDY
                     GCGVQYFSQQCVGRLLAPAGIEVDPKLPLPEKLKHVQQLMNQGDYRARKIYETIGAYL
                     GYAIAHFTDFYDLANVLILGRVTSGPGGDVILAGAREVLKVEFPELARQIAFHIPDEK
                     DKRHGQAVAAASLPKVG"
     gene            17471..18463
                     /locus_tag="PKI07_02130"
     CDS             17471..18463
                     /locus_tag="PKI07_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC49878.1"
                     /translation="MTTHRFNRLVLGLPRRLVLPIATYPGLALTGATVRQAVNDASAQ
                     FEAVAALHERYRTPVVLSAMDLSAEAEAFGCALHLADNEIPSVTGRLVTTLEQARALP
                     VPQPGDRRTAVYLETVRRLKQLPSRPLVLGGCIGPFSLASRLVGVSEALEMTLAEPEL
                     MAVVLEKSTAFLAAYARAFRQAGADGLIMAEPAAGLLSPRGVAAHSSGYIRHIAAAAG
                     GEDFGLILHNCAAKELHLAAILETGLSAFHFGAPMDLPSALGKVPPGVVLCGNLDPAG
                     VFCQLAPAEVRARAAELLSRAGAHRNYVLSSGCDVPAAAPLANLDALYAAASGA"
     gene            complement(18573..18743)
                     /locus_tag="PKI07_02135"
     CDS             complement(18573..18743)
                     /locus_tag="PKI07_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49879.1"
                     /translation="MPKVIYTANEANRGFISAMLAPVDARREPRFGMRQALWAATFVV
                     GAMVLMRLLLAG"
     gene            18921..19826
                     /locus_tag="PKI07_02140"
     CDS             18921..19826
                     /locus_tag="PKI07_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC49880.1"
                     /translation="MKRSASIHYLAAILILAVGGSGLLPLRAGAQGAAPAAASGALTV
                     VSYNLRFASPTPPNAWPVRRPLMVELIQKLAPDVMGTQEGIYSQLQDLAADLPAYQWI
                     GMGRDGGSRGEFMAVFYRPARLEPVAFDHFWLSDTPESIGSKTWGPRLARMVTWVQFR
                     DRQSGEQFVFVNTHLDHEVQAAREKGAQLIRDRVAAFKHQLPVLLVGDFNAAAGSNKA
                     YSILTGDQFFTDTWVAAPKRVNEGIGTMNHFKALQKNGARIDWILSRGNVAVERIEIA
                     TFSREGQFPSDHCPVVATLRLGARR"
     gene            complement(19945..21486)
                     /locus_tag="PKI07_02145"
     CDS             complement(19945..21486)
                     /locus_tag="PKI07_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC49881.1"
                     /translation="MELSRRSLLVYGLLGALWVLVLGWQTQEHVRVREAARTGLRNRA
                     KDIANTLSAIIRGMRFRGVIPQERLEPVLNELIHGRTNELIQSSELLSIVLLNATNQP
                     VASAGQPIDLQRKDLLQEGEHWGARSVTLVNPVDLGASLSALGVTNTVILPLPGEFTN
                     RGSEPGHEFTRRGPPPPEEMLPPRTPPPPDAGEFQPGGRPGRTREPGSRPRRPFWLRG
                     MAEEDFQSLVAKQTLHGLVLTLSTEGLAAAITRDLWLRTIIVALAGLSVLGSALAWRN
                     LAKSSELQIRLVRTSEMNTRLKEMNLAAAGLAHETRNPLNIIRGLAQMISRQTAAPAE
                     IQERSRAIIAEADKVAAQLNEFINYSRPREIRRTRLALNAVLNEVVRALNYDLEEKQI
                     NLQIKGEPLTVEADEQSFRQALFNLLLNAIQAVEPRGEIQIAAEKRNAAEATLEVRDN
                     GPGVPPGHRAEIFKPYFTTQKQGTGLGLAVVQQIVLAHGWEVECLPHEPRGAIFRISH
                     LKLAA"
     gene            complement(21532..22794)
                     /locus_tag="PKI07_02150"
     CDS             complement(21532..22794)
                     /locus_tag="PKI07_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49882.1"
                     /translation="MRKTNIQWRAGLAGSLALALALAFPLHAQQRSGFVGGAPGGAGA
                     RGGMSGSSTSRQYPNNTTIGDAYFSIDPETRRVVYIADEATAQQISRVLTNLDRPKPQ
                     VLIKVVFLEVSHTRASDIGLEGGWGRQNMSGTVQDASAVNGFGLSGLSTSVGTNLNRF
                     GQPFSSFSAASPISQNGAGLYQILGQDYQVTLRAIAQAGNAKVLSRPSILARNNQPAT
                     ITVGQQVPLITGVRYDNYGNTINSVTYRDVGVILNVTPFITADGLVQMIVSPEISSVN
                     FTATTQISAGVNAPTIDIRSADTVVVTADGQTVIIGGLINSTKARSDTKIPVLGDIPL
                     LGNLFKRQQRSSNLNELLIFLTPHIVQAPTELAALTARERENSKVPQGLTEEELNQFL
                     DQLPAQKPLPRSNPRPGKRAPAPQPGDS"
     gene            complement(22849..24480)
                     /locus_tag="PKI07_02155"
     CDS             complement(22849..24480)
                     /locus_tag="PKI07_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOC49883.1"
                     /translation="MKTIHHLLLFAGLTAGVCAAGAAPAPDETPTNAPPAALADTKAA
                     PASPQPAAPASPAENAGAPAAGTNGVAAAAAALEPPVRIEQGTNGLRLNFHNAPLNLV
                     LDYLSDAAGFIINKEADVRGTLDVQGKDLTREEAVEVLNSALKKNGYAVIRNGRILTI
                     VAQDSAKTKDLEIVQGNDPEAVEKSNEVVTQIIPVRHANVNQLVPNLELLLPSTATLT
                     ANESASSLILVATKTDIKRMLKIISALDTSIASVSTIRVLPLKYADAKETATLITQLF
                     APPPGSQTSGRGGFFSMFGGGGPPGFGDRGRGSSSNNRSGPGSGAAAAQVVAVGDDRS
                     NSVVISAPADLLATIVTMVMEIDQEVTDVTELRVFRLANADPAEISEQLALLFPEPTG
                     ASGTGSTGSQMPFFFRGSTATRRGGGASDSDRAKKMGRVLAVADPRTSSLIVMASKTL
                     MPQIADMIAELDADKGRKEVVGFYDLQNADPQDVQAALEDLFNRNTRRSSSSQNSRLG
                     DNNPLTRRATQNTQATSGRTVRSTGAATRNTSGGF"
     gene            complement(24487..25482)
                     /locus_tag="PKI07_02160"
     CDS             complement(24487..25482)
                     /locus_tag="PKI07_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49884.1"
                     /translation="MSLLTERMSARRCRWRRAAGRAAGGILLAFLLVSGPPAAAQEPA
                     AENHTNDLSDSEMLARLGELLQAVESGQFDNLERPENLSATNEVSPASPAAPGASRPA
                     TGAAATNGTNRFPGSSRSQKDARHFRGRRSARPSDNTRSSSRPAGDPSPEGDRRPAAA
                     PAGTNSRPASLDFSAFKIIADRNIFDPNRYAHRAGEPRVRPAPKVIDTVTLVGTMSYA
                     KGWFAFFDGSSSEFRKALQLRDTIAGYQVAGIAPDNVALAGETNRLELKVGTQLRRED
                     GGPWRLASPAEANSTPAASTATNTTATAAAPDAAPAASDAESDIIKRLMQKRLEE"
     gene            complement(25497..26030)
                     /gene="pilO"
                     /locus_tag="PKI07_02165"
     CDS             complement(25497..26030)
                     /gene="pilO"
                     /locus_tag="PKI07_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOC49885.1"
                     /translation="MPIKNRQQFLLIAAGAALALLAGDRLVLSPLLKAWSQRAARLTE
                     LQKQITDGRLLQQREPSIRSRWQQISENTLPANTSAAEQQVFKAIDLWAQNSGVTIAA
                     ITPQWKQESDDYKTFECRVDVAGDLGKLSRFLYSVEREPMALKLESVELGARDKEGQQ
                     LSLGLQLSALVLTPQKP"
     gene            complement(26020..27480)
                     /locus_tag="PKI07_02170"
     CDS             complement(26020..27480)
                     /locus_tag="PKI07_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49886.1"
                     /translation="MIKLNRPDWFRRQRPSGVLGLALDGSRLEGLALRRANGALHVHG
                     AFSVSLSLDPLTNDPELVGREIRNHLEAQGIRERPCVVALPLKWALTTQVEVPELPEA
                     DVASLLQIEAERGFPCDVQTLHIATSRFQTADGKQHALLIGIPKNHLTALERALRAAK
                     LKPLSFALGVAALQPPGLAAADGVLALAISESHVGLQLTAGGGVAALRALEGALEVEG
                     SQRLLRADVVAREVRITLGQLPPGLRETVRRIRVFGPTHLSRQLADELELRLEAAGLE
                     VESVERYAANEFGSPAPPDTPVSPAFSLAAERLAERRAGFEFLLPTPTPWQQWAARYS
                     SGKLRTVAMAAAAAALVVGGLFLYQQVRLWRLESQWRGMQAAVRQLEDLQEQIRRYRP
                     WHDNSVRSLTILRHLTEAFPEDGAVTAKTVEIRGLNNVTCTGTARNYPALLKTVENLR
                     KVRQFREVALGPTRGQSPALQFTFNFQWNEGGQNAD"
     gene            complement(27473..28885)
                     /locus_tag="PKI07_02175"
     CDS             complement(27473..28885)
                     /locus_tag="PKI07_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOC49887.1"
                     /translation="MRLREKSPASGFARSARRCRAGSVLVIVLWIAFGLVSLALYFAN
                     AMSFELRAADNRVSAQAADQAIEGAVRYLNYLLAAQISNGSNGVLPSLEEPLCQAVPV
                     GEAHYWLISRDTNNPIGPGQLCFGLVDEAAKLNLNSAEGDTLIWLPRMTEDLTQAILD
                     WRDTNGNGPTVIYYAMQQPAYQCKCDLFETVDELRLLYGADMDTLLGEDANRNGVLDA
                     AETDDNQNNLLDPGVLEYCTVYSREPNTQRDGSARINVANLNGFAAQLGSLLATNFGA
                     ARADQILATVGLAGSRPGRAPGGGATGTRFTSPLAFYLGSGMTAAEFALISTNLTATE
                     GAYVTGRVNVNTASAAVLACLLEGDVAVAEMLVNYRQSNPDELTSIAWVADALRQNYP
                     EALQTLAARDVLTTQSYQFTADVAALGPFGRGYRRVKFVFDTSSGSPVIVYRQDLTHL
                     GWALGPEVRQDWLLAGTQHD"
     gene            complement(28882..29658)
                     /locus_tag="PKI07_02180"
     CDS             complement(28882..29658)
                     /locus_tag="PKI07_02180"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HOC49888.1"
                     /translation="MNRTRNLKLKTAPRPGRIAAVRTGKAVSRSGAQAFTLIEMLLAL
                     AICAIVLVAINGVFATALRLRDNTSGALEESLPVNRALALLYQDLKGTVNPGRSLAGD
                     FRCGVPAVGATMGLSGEAGSAGLDFYTTTGTLSDRAPWGDVQEVFYELKAPANREQAG
                     MDLVRCVHRNLLATTILAPDIQRLMSGVQTLEFDCYDGAQWRDTWDTSNTDTNLPVAV
                     RVRIQLASRPGQAGGEPSPIEMVVPLAPQTHARAMAEESL"
     gene            complement(29670..30101)
                     /locus_tag="PKI07_02185"
     CDS             complement(29670..30101)
                     /locus_tag="PKI07_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49889.1"
                     /translation="MKSAAPMTSRRRLAGAKRLSEAAFTLAEMLAALLFMAIVIPTAL
                     GGLHIASLAGTVAERKGEAVRVAQRLLAESLLATNWNQSVQSGTLTEAQRQFRWTLQN
                     DPWDQDPNQEAMRLLTVEVTYTARNRDYSVRLSTLADSTLP"
     gene            complement(30076..30693)
                     /locus_tag="PKI07_02190"
     CDS             complement(30076..30693)
                     /locus_tag="PKI07_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC49890.1"
                     /translation="MNAQPDNRARMRADAGAGGNGRRRPNGFTLIELILVMALLTVVV
                     ALTAPKLSRFFHGRTLDSEARRLLALTRSGQSRAVSEGVPMDLWVDAAAGEFGLAAEP
                     SYEPSDPKAVEFALDSDLQIKVIGGTAAAAPASALPHSRETRRASAPRVALARAHLPA
                     IRFLPDGTVGDTSPVKLQLTSHDGGSLWLAQSRDGLNYEIRSAHD"
     gene            complement(30690..31136)
                     /gene="gspG"
                     /locus_tag="PKI07_02195"
     CDS             complement(30690..31136)
                     /gene="gspG"
                     /locus_tag="PKI07_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOC49891.1"
                     /translation="MRLNPLNKNRTAGGRARGFTLVELLLVLVILGILAAIVLPRFTN
                     RTRQAQIAATQTQIATFKTALEAYEVDNGYYPRGRNGLMDLIQQPRDAQNWRGPYLQA
                     DALPKDPWGNDYIYECPGRHNPTSYDLSSPGPPGENMVIGNWTLKR"
     gene            complement(31156..32397)
                     /locus_tag="PKI07_02200"
     CDS             complement(31156..32397)
                     /locus_tag="PKI07_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC49892.1"
                     /translation="MPFFHYKALQANGAMTQGELEAAGRAEAFAQMVELGLRPVSLAE
                     KAGADTGVALPASLAALSFGKRSARVSSRELENFTRLLSSLLAAGVPLSRALVILYRE
                     ASSPAANAKWREIHDLVVDGMSLADAMGKSPETFPRVYTAMVQAGETGGFLDLVLAQI
                     ADFQAREKELRSKVLSAMLYPSILFVLAIGVLIFLLTFFIPRFQGIFKGFNAALPLAT
                     QVVIGVSNLVRGYGLFVSVGLGVAAFMLRTWFASEKGRRVWEGLVLRSPVVGPLVAQF
                     AMARFCRMLGTLLGAGVPLVQGLNVARKSIGNQILVDAVAQSIERVQQGGELGTSLAE
                     TRGLFPGSVVEMISVAEESGRLDQELVRIANVTEGDLDRELKTAVALMEPLMLFFIAA
                     FIGTIFISMVLPIFTLQDYIK"
     gene            complement(32409..34166)
                     /locus_tag="PKI07_02205"
     CDS             complement(32409..34166)
                     /locus_tag="PKI07_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOC49893.1"
                     /translation="MSELAAQEAAWKQLLDRMVAARQLAAADAATLLRQNPGGTQAPL
                     HEEADVLRWLAREYDLAYTTLEDVEPDRELLSLFPARILLKEGLLPLRRSNGAVEVAT
                     SRLFATQGLDTLKTLTGLPLKPVLAASEAIQREMKKRLGVGADTLGTLNEEASFMVVD
                     ERHEEDTDLDAAAEEASIIRFVNQVLRDAIELRASDIHLEPFEEEFRIRYRIDGVLQD
                     VPVPAQIKRFQPAIVSRVKILSHLNIAEKRLPQDGRIKVRIEEVEVDIRVSVIPMLHG
                     EALVLRLLRQNATLRGMGELGMAERELGCFRRVLQLPHGIILVTGPTGSGKTSTLYTA
                     LNEINDADRKIITIEDPVEYQLRGVNQIQVSEKAGLTFARGLRSILRHDPDVILIGEI
                     RDQETAQIAVQASLTGHLVFSTLHTNDAPGALTRLVDMGVEPYLVASSLEAVLAQRLV
                     RVLCERCKQPDDSPAARAFKGQVGIPLATVIYRSVGCPACRQTGFYGRHAIFEWMDSD
                     DEIRQLILKNASSDAIRDAARRAGMQTLAEDGWRLVRLGRTTVEEVLSVTTAKEIERT
                     TQKTALAGAGKEPAARSAP"
     gene            complement(34258..34800)
                     /locus_tag="PKI07_02210"
     CDS             complement(34258..34800)
                     /locus_tag="PKI07_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49894.1"
                     /translation="MNQWLVMAGLAAVMSLGADQVVAQPGGAPPGGRPGRGNFDPAQF
                     QQRMMERTREMLEVTSDEEWKAIQPRVQKVRDLQREAFSGLGRGMMGRGPRPGDGAPP
                     SDPGAARRGGFFGAPSPEAEALQKAIDAKAPKAELKAALEKYAASRKAKQAEMEKAQA
                     ALRELLTPRQEAIAVLNGLL"
     gene            complement(35033..35998)
                     /gene="rnhC"
                     /locus_tag="PKI07_02215"
     CDS             complement(35033..35998)
                     /gene="rnhC"
                     /locus_tag="PKI07_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOC49895.1"
                     /translation="MKPLTSYTGKLTEAQAAALRSWLQAHFYQFRPVPYARFAADKEK
                     TSVVFYESGKLVVQGKGTQEFVEFVLEPEILKEARLGYEAVLNPDLLLPRLGVDESGK
                     GDFFGPLCIAGAYANASVVKAWAEAGVRDSKNITSEKRIKDLAELIRKTPGGVTTVVP
                     IGNEAYNRLYGKMGSVNAILAWGHARVIENLMQQRQRMNPPPVRAISDQFAHSKETVA
                     RALMGLGREIELVQKHRAEEDLTVAAASILARHEFVTRLGALEKQCGRKLPKGASAAV
                     DAAAREIVDRLGAEGLAKVAKMHFRTAYRAQGLPEPPKTEWRRRA"
     gene            36146..37231
                     /gene="mutY"
                     /locus_tag="PKI07_02220"
     CDS             36146..37231
                     /gene="mutY"
                     /locus_tag="PKI07_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOC49896.1"
                     /translation="MKNGDIVQKLLAWFARHARDLPWRRTRDPYAIWVSEIMLQQTQV
                     KTVLPYWRRWLRALPHLAALADAPPATLHKLWEGLGYYARVRNLQRAAQIIQQQHHGR
                     FPRDFDAVRALPGIGRYTAGAICSIAFNQPRPVLDGNVMRVLARLYGVAGNPRAGRAN
                     ARLWELAEALVRRAAGLRARRGCAALNQSLMELGALVCAPRQPRCALCPVSGACVAHR
                     TGRVAELPERPRRTRPTPRRFMAFVVERHGRVLVRQRPAGGVNARLWEFPNVEVAPGD
                     SDARAAARKALGRLPAALEPLAVLPHTITRYRITLAAYRAAWRGETTPSRAGARWVER
                     RALNQLAFAGAHRKILRQLQAGPGKPS"
     gene            37340..37747
                     /locus_tag="PKI07_02225"
     CDS             37340..37747
                     /locus_tag="PKI07_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49897.1"
                     /translation="MKFLILLSLVLCLPGAGSSRADETNAPALTLSAAQAGEYIGKQA
                     TVTGVVAQVSIRPSITFLNFDKKYPASPFAAIIRSRHTNQFENLPNLSGKAVAVSGKI
                     IDYNGKAEMELTRKTQLKLLTNAPPPAPAPPAP"
     gene            37900..41463
                     /locus_tag="PKI07_02230"
     CDS             37900..41463
                     /locus_tag="PKI07_02230"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOC49898.1"
                     /translation="MMTEMQPGSTSRKPRRWLRALAWGGGLLAALLLLVYFIATSSGF
                     FTRVILPRVSRAMNAQITVRKAEIRPFRQVVLHDLKVQTTGAEPLATVAEARLRYHLL
                     AILGGNFRVDELTLASPTITVAENPDGTSNLDPLWATPAAPPVEKSPPPAPAPARPLR
                     VDLKQLALRDATFRQVKNHAQGPPDLLELARVNVTVEDLKNGQPGRLTLAGDLRLTGS
                     NALLHAQLEGRYDFALAADLQPASLKGSARLDVRRAENALAGFAGVHGALDAEVTPTE
                     VKEAALRIHKNDVSLGELRASGPLALEALEGRLDLSLTGIDHQLLNLFGAPHGLDFGP
                     TTLRSTHQIELSRGGTNITARGQVEVSRFQVTRAGQTTPQFDLHQEYDLTLDFGQKTA
                     LARQLALTGTQNGRPLLQGDLTHPMPLSWGDTHSSLGDSTFSFTLTDLNLADWKPFLD
                     EVATAGRVTARAQILSQQGGRQITFNLDSQIDHLATQVGGQTLAEAAVTLQASGQATD
                     LNQFNLASFKLEIAHRNQMLAAASGSGVCDLAAETADLKLAARAMLAPLLQALPQPEI
                     NLASGTLELNARLARQPQSQTLAGQATLADLTAQWGENAVRHLGARADFDVGLTPREI
                     QVRQLAGTLTQDARDGGRFDLAAKYHREKETVELTARLTDFNQHGAGPFLEPLLANQK
                     LVSVDINAQATAQYDPRGASAVKADLQVANLVVADPKQPAPGAPLAATMRLDASARQQ
                     KAEVRQFQLALTPTARATNQLQLTGWVDWSRTNALQGDLKLTAEALDLTRYYDLFMAD
                     TPAAARETPAAPDASAPAAASPEPEALTLPFHHFTAHAAISRLYLRELEIADAQATVR
                     IDGGRVVVNPCQLTLNGAPADATADLDLGVPGWKYALAFNAQALPLPPLINSFQPDLK
                     GQLSGTASAQGNLNGTGFTGASLQKHLAGQFDMTLTNLNFALVSLEGKTWYTRVLKTV
                     VNVIGTLPELAQNPAGTVLSLLTGKGSASGGGAPELKQSRLEAVALRAAAGGGRVDVQ
                     NATLQGSAFAAQAAGTIQLAEVLTNSSLHLPVAISLERTLAQRLGLAGDTPTNAVYAR
                     LPDFLTVQGTLGNSKASVNKAALLGGVLQAVDSRKSPPAAGGDTNSPAAPRQPGLDLG
                     RLLQGRGARPDPGAPAATNAPATNPAPVNELLRGLFGPDKK"
     gene            complement(41592..44348)
                     /gene="ppdK"
                     /locus_tag="PKI07_02235"
     CDS             complement(41592..44348)
                     /gene="ppdK"
                     /locus_tag="PKI07_02235"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOC49899.1"
                     /translation="MAKTTKYVYLFGNKKADGNGSMKPLLGGKGANLAEMSRIGLPVP
                     PGFTITTEVCTYYYQHKKTYPKSLEPAVKAGVAFIEKIMGTKFGDKKAMPLLVSVRSG
                     ARDSMPGMMDTILNLGLNDETVQALAAATKNDRFAWDCYRRFVQMYGDVVMGVQKRAN
                     EDHEPFEVVIHGFKHEKYHADVEDSKLTADDQRELVTRFKALIKERTGKEFPQDPWKQ
                     LWGAAGAVFGSWMNDRAIVYRRKYGIPSEWGTAVNVQAMVYGNTGDNSGSGVAFTRNP
                     ANGVKEFYGEFLLNAQGEDVVAGVRTPEPVAQLANHLPQAFKELNQIRGTLEKHFKDV
                     QDFEFTIQDGKVFMLQTRNGKRTALAALKISMDLVKEKLIDWKTAIRRNPSEQLEQLL
                     APIFDAKAEAAAKVIARGLPAGPGAASGKMYLNAERAVEAAGRGEKVLLVRNETTPED
                     LRGMIAAEGILTAKGGVSSHAALVARQMGKVCVCGAGALQLDYDERTLTVNGQTWKEG
                     DYLSINGTLGHVYAGEIKTAASEIVQVLIEQTLAGKDSETYRMYKTLMDWCAKVTRLQ
                     VRTNADTPEQTENAVAFGATGIGLTRTEHMFFEGDRIDAMREMILADTVEERKKALAK
                     LLPYQRADFVGIFKALKGFPATIRFLDPPLHEFIPHEEAAQRDLAAKMGVPVEKIEQR
                     VQELHEFNPMLGFRGCRLGVVYPEISEMQARAVFEAAAEVQKAGLKAKPEIMIPLVGF
                     KRELDLQAEVVHRVAREVMAERKVKIQYLVGTMIEIPRGALTADEIAQTAEFFSFGTN
                     DLTQTCLGMSRDDSGTFIPAYTEAEVVKANPFATVDQVGVGELMKIAVQRGRQTRPDI
                     KLGICGEHGGDPNSVKFCHRIGLTYVSCSPFRVPVARLAAAQAALEEEAEAKAKGARK
                     GKKK"
BASE COUNT         7362 a        15385 c        14396 g         7886 t
ORIGIN      
        1 cgcaaaagct tcggcggggg ggaacctgaa tatggaggcg ccttcgcgcg gttggcgttt
       61 aacccgtttc cagaggtgca actaccgccc gggcaaaggc ttatttccgc agggaaccga
      121 ccaggccgca acgattgccc gctgccgggg ccaaaagcct tttccccgcg cacggtcttg
      181 cggccagacc gctatttttt gtcctttttc ttctcgtcct gcttgttcgc ggtcttttca
      241 tccgtcttgg cggcttccgc gggcgcgccc acattgagtt gcgccaggag cgcgtcggtc
      301 agatcgtttt cgccgctggc gtagagcaga atcggagtgc cattggcgct ttcggcggcg
      361 gtgtcgaaca cggttccata gccggcggag accgccttgg ctttaacgac ggcgcggatt
      421 tcctcgacga gcttgtcccg catgcgtttg cgctgttcgt ccagggaggt gcgagcctgg
      481 cgctcatatt gggcaatggc ctcttcggaa tctttaattt gcttgaactt ttcctcggcg
      541 gactttttgc gtttatcgcg ttcttcgacg gaaagggtct ggttgctggc gtcagccagc
      601 agggcctggt agtcgtcctt ggcctttttc cagtcctcga tcatggtttg gtggtctttt
      661 tcgatgtcgg cggcgcgttc cttgagcgcg gcgtcggctt gtttggtctt ccagtagttg
      721 tcgaagagct tgcgcagatc aacggtggcg atacgccctt gcgcccaggc ggaattgccc
      781 agcaaggcaa ccagcagcaa cgccggaaga gtttgtttta acagactttt cattgtgaat
      841 tgagccaatc agagttttcg caagccggca actcgaaact gcctgtggcg gccggcgtct
      901 caagcatgag acaacggcgc ggcgtccgcg ttcgatccgg gtcggggaaa atcagaacat
      961 gcgctcccag ccgacgccaa actggaattt gccggagccg ctattgtatt tgtcgtggtg
     1021 gatggggaag ccatagtcga gccgcagcgg gccgatgggc aggttcaggc gcaggccgag
     1081 gccccagttg tcgctgaagt cgctcaggtt gaggcggtat gaacggcggt ccacgctgcc
     1141 gatgtcgtag aagatcgcga agcgcacccc gatgccgcga tcctgctcga agatggggag
     1201 gctgtattcc gccgagccga accagtacgt gtcgccgcca atgggctcat tcccgcgcag
     1261 ctcgggtttc tggcgcgggc tgatgtagcg gtacttgaag ccgcgcaggt tgtagagtcc
     1321 gcccaggtag tagcgctcga agaacggcac gtcgtccgtc ccgcccaggg cttcggccac
     1381 gcccgaccgg ccacccagtt cgagcacatg gcccgggaac aggccgcgga agtaccaggc
     1441 gctggtcagt tccagcttgt agaaggtgtc cgagccggtg gtgatttggg ccgtcagctc
     1501 ggtgcgctgg cctttgttgg gcagccggac gctgttgcgc gtgtcgtacg cgagcgaggt
     1561 gcccacgcgg gacaggaggt ggtcgcccct ggcgtcccga atgtcatccg gcacattggc
     1621 gcgggggcct tcggtcacgg ctttatgggt caggtcaatg ttgacgttct cgagcgtgta
     1681 gttgacgccg ccgcgcagga actcgctgcc cagcgcgcgc tccaggccca cgcgcccgcc
     1741 gacgcgggtt tcgtcataca ggtctttggg gctcaggaag ctgtattcgt gatagtacag
     1801 gtcaacgctc aactggagtt tacgatccag gaaccagggc tcgatgaagg tgacctggta
     1861 atcctggcgc cgggtgccga ccatggcccg caggcggagt ttctggccgc cgccccggaa
     1921 ccagggaggt tggaagggat gggataaatt gaagttgcct tcgtagagtt cgacatagcc
     1981 gaccagcgcg tccacgctgc tgaaccccgc gcccagcatc acgtttccgg ttcgcttgtc
     2041 ctccacatcg aagacgaggt tcttgcggtt cgggacatcc gtgggctcgg gccgggcttc
     2101 cacgcggtcg aaatagctca ggccttcgag gcgcgatttg ctgagtttga cgcgcaccat
     2161 gtcgaacaac tcgccgggtg caacggccag ttcgcgccgg atgaccttgt ccttggtgcg
     2221 ggtgttgccg cgaatttcaa tcttctcgat ataggacggc tgcccttcct cgatctggaa
     2281 ctcgaggtcc atcgtgcccg tttcggtgtt ggggattttg accacccgca aattgcggga
     2341 cgactcctgc acgtcaatgt atcctttggc gccgtagaag tcctcaatcg cctccacgtc
     2401 cttggcgagg ccgccggcgg tgaatacgcc gcccacgtcc atcggcaggc cgttggggcc
     2461 gagggcggcc ttgctgcggc tgcgcttatg cccggcttgc aacccggcga caatctccgg
     2521 ggtgctgaac agcttgttgc cggtgaactt gatcgcgccg accttgtact ggttgccttc
     2581 aaacagggtg aaccggacga tcagggtgcg cggggtgggg ttgacgaact gcacgtcctt
     2641 gatggcgaaa tcaatgtagc cggccttgcg gtagaactcc gccagctttt ccttgtcctc
     2701 ctcgaactgc tcttccttga agacgccccg gcgggtgagc caggaaaaca tccagtgctt
     2761 ccgggtcttg atgaccttcc gcagtttttt cagggggaac gccttggcgc caacgaagtc
     2821 cacctcgacg attttgattt tggggctttc agtgatgaga aaggtcgcgg tggccaggcc
     2881 cgacggctcg tccaggtggt attcatattt ggcttccgtc cgcgaatagc ctttcttttg
     2941 atacagcttc tggagctcct gggtgtcggt gaataatttg cgcaggtcca gcggctcgcc
     3001 ggctttcgag gagagcttct tgagcagttg ggcgtccttg aaccgggtat tgccctggaa
     3061 cttgatttcc gtcaggcgcg gccggccctg caccttgtag gtcagggtca tgccctcggc
     3121 ggtggtgtcg gcggtgacct ggacgttgta gaagaagccg gtggcgtaga ggttgcgcac
     3181 gtcgtcatcc accgcggccg ggaggtagct gtccccgacc ttcgtgcgca actgggcgcg
     3241 caccagctca tcgctggtgg cgggcgggcc gatgtgctgg atgcggatgg cctggatttt
     3301 ggcggcggca ggctggcccc acccggctgg cagccaggcc agcagcagga gcggcacgca
     3361 aaggcgaagc aggagtttca tgggtgcgga ttcaattcgg cacgtcttcg tcgctgactt
     3421 tggcggcgct ttcaaaccgc gtataggatt tcaggaacat caaattcaca tcgccggtcg
     3481 gcccgttgcg ctgcttggca atcaacaggt tcaccggcac ggcgtcttct tccaccgcgc
     3541 cgccgccgtc ctcgtcgtcg ccgctcgctt tcggcttgta gagcagcccg accacgtcgg
     3601 cgtcctgttc gatcgcgccc gattcccgca ggtccgacaa ccgcggcggg cggccttttt
     3661 cgcgctcgat ctcccgattc aactggctca ggaccaccac cggcaccatc agttccttgg
     3721 ccagggcctt gaggccgctg gagatgtcgg cgatttcctg ctgccggttt tcggcgcgac
     3781 gggaagtaga atggagcagt tggagatagt caactacaaa cagtttgatg ccgtattgct
     3841 gggccaggcg gcgggcctgg gcgcgcagtt gcaggatcga gagggcggag gagtcttcaa
     3901 tgaacagcgg cgcgttggag agtttgcccg cggcggcggt gagcttggga aaatcgcgct
     3961 ccgccaggaa accttcgcgt atgctgcgca ggttcacccg cgcgcgcgag cacaacatgc
     4021 gcagcacgag cgattccgcg gtcatttcca ggctgaagac gccgaccggt tggcgctgct
     4081 cgagggcgac gtgttcggcg atgttcatcg ccagggaggt cttgcccatg ctggggcgcg
     4141 ccgcgatgac gatcatttcg ccgccgtgca gcccgctggt tatccgatcc aggtcggtaa
     4201 agccggttcc caggccgctg agctgcccct tcccctcgtg gaatttctcg atgatgttga
     4261 tggcctggtg caccagttcc ttgattttga ccgtctgcga ctggacccgg gattcgctga
     4321 cacgcaggat gttgcgctca atctcgtcca tcaattgatc cacttccccc tcgtgctcat
     4381 agacgcggtt gaccgtttcc gtgcagacct ggaccatttt gcgcagcagg tatttctcct
     4441 gcacgatgtc caggtaatac gccaggttgg ccgccgacgg caccgcgtcg gacaggctgc
     4501 cgaggtaggc gaggccgccg acttcttcga gcagttgccg gtttttgagg tgctgctgca
     4561 acgtgatgag gtcaatcgcc tggcgggcgt catacatctc gatcagcgcg ctaaaaatgg
     4621 tctggtgccg caggtcgtaa aaggcctccg cgccgcgctt gaacttcccg atgcactcgc
     4681 ccaggcattc gttgggcgag agcagaatgc agcccagtac gccctgctcg gcttccggcg
     4741 aatgcggcgg cagccgatca acccgggccc ggctcgcggc ggacgatgag cggcgcggcg
     4801 cccggtgcaa atccttgggc gcgccgctat tgccttctgt gtttgcgttt gtcatgccgc
     4861 taccaaacgt gatgttccgg ttgtgagcga atctgagtta ttgaatctta atcaccgggt
     4921 attcacaaga acgaagcggc gccccgatcc ggcgcgggtg cggccttcgt cccggaagcg
     4981 cgccgggcgc ggacgccgcg gcgaacgcgc cggaccaacc cggggccgaa attgcgcccg
     5041 gcgccccggg cttccccagg gctccgtcac tgccttgacg cccggcggga accttgcagg
     5101 aggtagcgga gcgggggagc atcccggggg agggcacggg gcgccgcgct taaatgagtt
     5161 cgagcgcgtc ggcgtccagt ttcacggccg cggcctggtt gatgaagtcc agccgcagca
     5221 gcacggtgct catgccggaa cgccgctcga cccagccctc gatgccttgc agcgggccgg
     5281 ttttgatccg cacgcgcatg cccgcgccaa tggcgggcgc caggcgcacg cccaccttcg
     5341 cctcgagggc caggagaatg gcctgcaact gccgctggaa ggtcgcctgg tcatagactt
     5401 cgagcaggtt ggccacatgg tcgttttgcc gcaccgcggc cttttgttca ggctccaact
     5461 ggagaaagac atagcccgga aagagcggct tatcgaaaac gacggtcttg ccccggtatt
     5521 tatgcgccga gcggtagcac ggcagaacaa cggccagttg ctgccgccga caatgctccg
     5581 ccagcttctt ttctctgcgc ggccgggtgt gggccacgaa ccaaaacagt tcggacatgc
     5641 gccggaggat tcgggccgcg agccgccgag tcaatccgaa ggtgcggcgg cggcgttcgg
     5701 cgccgcccga cgccggccgg ccggactgcc gcctggtttc aattttggcc ggcgggagcc
     5761 agggcctcga ggtgacgccg ggcgtcaggg tagtcgggcc gaagccgcaa cgccgcccgc
     5821 aggtgctccg ccgcctcttc gcggtggcct tggcggagca gtcccagcgc gagattgaag
     5881 tgggcttcgg ggttgtcggc ccgcaggcgc aacgcctccc gatactgcgc gatcgcctcc
     5941 gggatcttgc cctgctccgc cagcacgttg cccaggttgt tgtgggcctg ggggtcttgc
     6001 ggcttgagcc gcagggcgat ctgatattcc tggatggcct cgtccagttt atgttgcgcc
     6061 gccagcgcgt tgccgaggtt gctgtgggcg ctggcgtaat tggttttggc ccggatggct
     6121 tcgtggaaat gggggatggc ttcgtccagc ttgccctgca tggccagggc aatgcccagg
     6181 ttgttgtgcg cgtccgcgtg gccgggtttt tgttggagga cgaacgagta gtgctggatg
     6241 gcctcgtcaa tccggcccac ctcggagagc gcattgccca ggttgttgtg gacctcgata
     6301 tgtttgggcc gggagcgcag cgccgcctcg tagagggaga tggcttcggc aaactgccgc
     6361 tgggcggcca gcgcatagcc gagattgttg agcgcatcct cgtaggtcgg cttgatctgc
     6421 agggcccggc ggtaatgctc caccgcttcg gccgtccggc cctggccgtt cagatagtag
     6481 cccaggttgt tgtgggccag gtagttgtcg cgcgtcaccc ggacggcatg accgaagagc
     6541 tcctcgctat tgcgccagaa tcggacctgc cgcgcggcca gcaaggcgca agcggccaac
     6601 cccagccccg cccccagcgc cagggctttg ccgcgcccgg gccgcgccgg caccagcgcg
     6661 tcaacgcccc acaccagcat gatgaacccg ccgatcaagg gcacatacgt atagcgatcc
     6721 gccatggatt ggatgcccac ctgcaccagc ccaatcaccg gcaccagcgt gccgcagaac
     6781 cacagccagc ccacggccag gtagggctgc tttcgcgccc gcagggccac caccaccgag
     6841 atggccagca gcagcaccgc cgcccccgcc accgctccca gcggccagcg gcccggatgc
     6901 gggtagagaa tcgaaaggtc caacgggcag aacgtcttgc cgatatagcg agcgtaggaa
     6961 atgagcgcgt tggtgacccg ctcgccaaac gtcaggctgg tcgaaacagc gccgccgtgc
     7021 cgctgcacga agtaagtgac gatgctggaa gcggccgcca gcgcaaagaa cggcgctttc
     7081 tccagcagga ggaaaaataa acccgggcgc ggccggccag gcgccgtgtc cggttgcgcc
     7141 gcgggcgccc ggcgcggcca tcgctgcagc ggccagtagt ccaggagcaa caacacgaac
     7201 ggcagcgtca ccagcatcgg cttgctcatc aggcccaggg cgaaacaacc cagggccagg
     7261 agatagttcg cgacggggcg gcgcgcgcgg cccggtctcg cttccggccg ccggccttcg
     7321 gcgtaggccg catacgccca cagcgtcagc agaaagaata acgtgctgag cacgtccttg
     7381 cgctcggacg cccaggccac cgattccaca tgcagcggat gcaacgcaaa cagggcggcc
     7441 accaacgcgc tgcgccagag cgcgcccgtc atgcgccgca acaacccgaa cagcaggagc
     7501 gcattggcaa tatgcaggcc cacgctcacg agatggtgcc cgccggcctg gaggccaaaa
     7561 agctggcaat ccgccatgtg agaaatccag gtcagcgggt gccagttgct ggcgtggccg
     7621 gtggtaaaag cccaaacaat actcgcccac ttcagcccgc cctgaacctg cgcgttctcg
     7681 gtgacgtagt ccgcgtcgtc atagttgacg aagccatgcc acgccaccgg cagaaagacc
     7741 gccagggtcg ccagtgccag caacaggcac agcacgcccg gccgcctcag ccacgcccac
     7801 ccggaggcgt ccccgacgcc tgattcagcc gcggcggcgg cgccggtctt ggttcgcttg
     7861 ctcattgcgg gcgatattta agccaggaaa aagaccgccg tgaaatgaaa atcgcgattg
     7921 gcattgcggc cattaacgag tatggtaggt cgcgaatggt gaacttcggt gcaatcgcgg
     7981 caggaagcgg atgcgagccc catgtgcggc cagcctccgt ccgggtccta ttgtgccccg
     8041 gcccggaacc acaattcccg gccccctccc cggtccccgg cgcggcggcg gcgattgacc
     8101 tctcccatcc caggcaataa gggtctccgg gcctcgctcc cctctcgcta tggttggcat
     8161 tgatttcatt caggatttgg ggctggtcat gctggtagcc gccactgccg gctggctgtg
     8221 ccggcggctg gggcttccct tgatcctggg ctacatcacg gcggggattc tgattggccc
     8281 gcacatgccg ttcttcgtca tggtggatga caccaaccgg atccacgcgc tggcccaggt
     8341 cgggctggtc tttttgattt tccagattgg ccagggcctt cagttgcagc ggctgaaaag
     8401 catgggcctg cccctggtcc tggcaacctt catcattgcc atcttcgtgt ttaacggcag
     8461 ccggctgctg gcgtacctgc tgggctggcc gcccgtttac ggcctggtcc tggccggcat
     8521 gctgatggtt tcaagcaccg ccattatcgg aaaaaccctg cgcgaaaata acgccctgca
     8581 cagcatcttc ggccagacgg cgctgaccgt gaccgcgctg gatgatttgg tggcggtggt
     8641 gatgctgacg ctgctcagcg tcatacaaac gtccgcggcc gtggcccccg gcgcgctgct
     8701 ggagaccatc ttccggctgg cggcggtcat ggcgaccatg ctcatcgtgg cgctcctggc
     8761 ggtgccgccg ctgattaagc ggctcaaccg caacacctcc ggcgagctgc gcacgctctt
     8821 catcgtgggc ctgctgctgg cgatggcgct gctgtccacg aaggccggct tctcggccgc
     8881 gattggcgcg ttcctgctcg ggagcgtcgt ggccagcacc ggccacaagg cgcaagtcga
     8941 gcaatccctg ggcgggctgt gcgaggtgtt tggcgccctg ttctttgtgg cggtgggaat
     9001 gctcttcgaa ctcaagctgc tggcgggcgt ctggccgctg gtgttgctgg tgtttgcttt
     9061 cgcgctgctc tggcggagct tcgcctccag cctggcgctg ctggtggtgg ggcagcagac
     9121 cggcgacgcg gtgcgcgcgg gaatctgcct gacgccaatc ggcgaatttt ccctcatcat
     9181 cgcgctggca gccgtccagg cccggatcgt gccggattgg ttctacgccc tggccatcgg
     9241 cctgtgcctc ctcagcaccc tgacgacacc gatcatcatc cgccaccccg gccggttcag
     9301 cgacgggttc gaacgctggc agccggagtt tgtgcgcaaa gcggtggagt tctatcacga
     9361 ttgggtggaa agcttccgcc atcgccagcg ctcgagcctg ttctggcggc tgaccggccc
     9421 ccgctggctc cagctcgccg gcgaagtcct gttcatttcc ggcctgttga tcttcgcgca
     9481 accggggtat cgcctgatcg cccaatggct ctccccggat tggcccgcgc cgatcggctt
     9541 gccggctctt ttctggctgg ggttcctcct gttgctcctg gtgccgctcg tggcgctgtg
     9601 gcgcacggtg gaagtgattt ccatgatttg cgccgaagcc gcaacccgcg ggcaccggca
     9661 ccgggacaag ctgcagccgg ctttcgagaa actgctgcac ggggcggctt acgtgctgat
     9721 cctggcctgg gtctcgatct tcgtcccgta tgaatcgctg ccccggaaaa gcctgttcgc
     9781 cttcctggcg gtggcggtgg ccctgaccct cctcttctgg cgcaagctgg tccgctggca
     9841 cagccgtttc cagattgacc tgcgcgcgca cctgcagggt tccgcgttcg ccgccccggg
     9901 cgtgtcccgc gcaaactggc agcagcacag cgagcgctgg catttgcgcg cccgcgagca
     9961 tgtcgtgggc gagcaaaccc gggcggcggg caaagccatc catgacctgc cgctgcgcca
    10021 gatgttttcc gccaatatcg tcggcatcga gcggcagggc taccatctgc ccaaccccga
    10081 gccgaacacg gttttatacc ccaacgacaa gctgctgctc ctgggcgcgg aaaaagacct
    10141 gcgccaggcc gaacgctggc tcagcgccct ggaaccccaa cccgcatcgg gaaacggccg
    10201 cgagccctcc ttcgcggacc tgtgcctcga gccgctcctg gtgccggcct cctcccggca
    10261 tatcggcaaa tcgctgggcg aactcaaact cacgcagcaa ctgggcatcc aggtggtcgg
    10321 actcgaacgc gaagacgata cgcgcctcac ggtcggaaag ttcgacaccc tcaaggcggg
    10381 cgaccggttg ctggtcctgg gcacgcaaaa acaaatcacg gacatggcct tctggcttgc
    10441 cacctgattc tctgctcgcg aacgaacctc acttgaacgg aaaggaaaaa cacaatgacc
    10501 gctcctgcta aacgcactat ctacatcacc aacgcggata tgatccgcct gcgctcgctg
    10561 gtcgcctccc ccaagcatgg ccgggacgac ctggacagcc tgcgcagcga gctggatcac
    10621 gcgcgcgtgg tcgagccggc cgacttgccc gccgacgtca tcaccatgaa ctcccaggcc
    10681 cagctccgcg acctggaaag caaagaggtc atgaccttca ccctggtctt cccggaaaag
    10741 gcgaatcccg agcaggaccg gatttcggtg ctggccccca tcggaaccgc catgctcggc
    10801 cagcgcgaag gggacgaatt tgattgggaa gtccccgccg gccgggtgcg gctcaaggtc
    10861 ctcaaggtct tgtaccagcc cgaggcggcc aagcattacc acctgtaatc ccgcgcccgg
    10921 cgggcccgtc cgcccgcaaa ccggccggcc ccgggaccgc ctgcggcttc gccgcgaaca
    10981 tcctcaaatc cgcaaccgcc ggatttgagg atgcccgctc acggggcgct ccccggcggc
    11041 ggccagccgg cgcgcccggc ccggcccggc gcgcggccgg tcagatcggg cctccttccc
    11101 acagcgcgcc ggcacggttt gcaccggcag tgaaacgaaa tgttccgcca cccggacgcc
    11161 agcagcgcca aaccctcccg ccaccggctc aactgtgttg gcataaaact agcatgaggc
    11221 gggagggtag gccggacgaa aatttggcct ggcagtggcg ttgagccgag acgacgatcc
    11281 ccggtcgggg ctgcccgaat gcagcctgct gcctggtgaa cgcaaccaag cctggcccca
    11341 cctgcggatt acgccggcga gatcatccgc aggtggttta atgatgcgat aaaccaaacg
    11401 aacccggaat gaaatcgagc cgccaggaac tgcaagagct ccgcgtggaa gcgaaatacc
    11461 tgctcagcca cgaccaggcc cgccagctcc ggcccctcct gcggcggcgc ctggcgctgg
    11521 acgcgcacgg cgcggcgcac cccgacgccg gctacgatgt ccacagcgtt tacctggatt
    11581 ccaaccggct gcatacctac tgggcggcgc tcaaaaaaga aaaagaccgc gccaagctgc
    11641 gaatccggtt ctacgatgac gaccatgccg cgcccgccta cgtcgaagta aaaacccgcg
    11701 agggaggcca gaccatcaaa cggcgctgcc cggtgcggcg ggaccagttg ggcgccgtga
    11761 ttgccgggcg cgtccccgga ccgctcgccg gcgaaaccgc ccccgaaggc cactcccgcg
    11821 ccctgcagca tttcatcggc aaattgctaa aactggacgc cgggccaaag ctgcacatcg
    11881 cctaccgccg cgaggcctac gtctcgaaaa acagcaacgc cgtgcgggtg accctggatc
    11941 gcgatgtccg gtccgagccc gtcaccgcgt tgcgcctgac caccgcgatg cgaaacccgc
    12001 agccgctttg tggaaacaac gtcgtgctcg aagtgaaggt caccggcccc atgccaccct
    12061 ggctgcggca actggtggcc gggttaagct cgccccaccg gcgcttcgcc aaatacgtgt
    12121 tcagcgtgca gcagcatggg ctgcaccggc aatcccagga ctgaccggca gagccgcccg
    12181 gcaacccgcg ccccgggcag aacgccgccc aagccgcttt ccggggagaa tggtttgagg
    12241 atggtgcgcg atagagggtt tgaacctctg accccttccg tgtcaaggaa gtgctctacc
    12301 actgagctaa ccgcgcaacc actgccacag tatgggtttt gcgccgcgcc cgcaagggca
    12361 aatcacgccg cccccaatgc gttgccccgc gccccggccc ggcgaaacgg atcgcctcgg
    12421 gatcaccccg ccgccggctg gcggtccgcc aggcgctcca gcacctggca cagggccgca
    12481 agccgcggaa cggccacgcc cgcgcgaagg gcctggcgga gaggctcctg gaaaaggctc
    12541 tgcaactcga gcggctggcc gcgttcgaaa tcaatcaacg tcgaagcctt gtaggcgccc
    12601 atcgcccggg tgcgctcgat cagcgggccg gcaaaatcat ccgggaccgc gtgccccagc
    12661 gcgcgcgcgg cggctatgac ttcgcgcatc aactcgcgca ccagccggct ccagcgcgca
    12721 tccgccaaaa gaatgtcagt ggtgagacag ccgcccggcg gcgatcccgg cgggagggca
    12781 tcctccgcgg aaccttccga acccgcaagc tggtgcagca cttccgggcc cgccgccccc
    12841 gccacgccca gaccattaaa agggatgttc cacaccagct tctcccaatg cgtccgcgcc
    12901 aggtcgtccg tgacggtgca gggaacgccc gcctcggcga agcgagccgc aatcgcgcgc
    12961 gtgcgcggcg ccggcggacg ctggaattcg cccaggacca cctgtccgaa cccgatatgc
    13021 cgaatgacgc ccggcgcgat ccggttcaaa cagacgaagc acagcccgcc caggatctgc
    13081 gccgccggga aaaggcgggc aagctgttct tcattgccca aaccgttctg gagcgtgagc
    13141 acggcgctct ccggccccac caacggcggc agcagcgcgg gaaactgtcc gttggcggtg
    13201 gtcttgagcc cgatgagaac caggtcggcg gggccgattt cctccgggcg ccgggcacaa
    13261 tgcgggtgga cctggaactg gccctccgga ctctgaatcc gcacgccctg gcggcgcacg
    13321 gcttcataat ccgaccgcaa aaggaaatga accgcatgcc cggcccgcgc caactgggcg
    13381 ccgtaatagc tgcccaccgc tccgcagccg acgactgcga tcttcaccgt ccgaccaaaa
    13441 aggcaccctg ggcgcgcccg ggtgccgcaa caaccacggg gaaactattt ggcgcccagg
    13501 atcgcatcct tggcggcctt ggagacgcgg aacttcacca cgcgcttggc ggggatttga
    13561 atggtggcgc ccgtcgcggg attgcgcccg gtgcgggcgg cgcgattcac cagcacgagc
    13621 ttgcccaggc ccgggaaggt aaaggtgttc ttggcctgct tgtaagccag ctcacagatg
    13681 gattcgagga tctggacggc ttgctttttg gagatatcgt tcttttcagc gatcgcggcg
    13741 gcgatttgag atttagagag tgctttagcc ataataactg tcttgtttat tgttgttagt
    13801 tgctgcctga agtctttcga ctaagcgcga attaacgctg tctgccgcgc cgctgcaagc
    13861 acaaaatgca aattattgtc cgccgcccgg ccgccccgat cgcccgcgct ctcccgcccc
    13921 ggcggccggc ccgctccccc gccgccggga gcccccccgc gcgcggcgct cagtagcaaa
    13981 ccttgtccgg cttgcgctcc cactcccgga aggcggccag ggcctccggg cgcaggagct
    14041 gccggagccg gcgctggcgc ttcccgatgg gcaacgcaac ctcgcggtag atgaagtcgt
    14101 cgtcaaagcc aatccgggcg gcgtcccggc gggtattgcc gtaaaagacc tgccgcaggc
    14161 gcgcccaata gatggccgcc aggcacatcg ggcagggctc gcaactggtg tacagatcgc
    14221 actcgtcaag ctggaagctc cgcaaccgcc ggcacgcctc ccgaatggcg gcgatctccg
    14281 cgtgcgcggt cgggtcgttg gccgtggtca cccggttcca gccgcggccc acgatcttcc
    14341 cccggcgcac caccaccgcc ccgaagggac cgccctggcc ctggcgcatc ttttgcaccg
    14401 acaggcggat cgccgcccgc atgaatttct cgtgcatgcc cgctccggcg ccggggaacc
    14461 ggctcaggca aacagctcgg cgatgttcaa agacagcggc gcctgtttgc cgccctcata
    14521 aaacctcacc gcctggccct ggctccactt gcgcaaccga tagagcaccg caaacgtgta
    14581 atccggcgcc gccccgccct gccccccgac gacctcgccg ccccggcgca cgttgtccat
    14641 catccacggc ggcagcagca gatggtaggg attgcgcttc acctcgccca cgtggaagct
    14701 ggtcgccgcg atcatatccg ccaggtcttc ggcgctgact tccaccatca ggttcggatc
    14761 cgtcatcgcg ccccaaaact cggtctcgac caggtagcag gcatagctgg cgggcatctg
    14821 cttcaaggca tccaccacga ggtggtgggt gccaatgtgc gtcccgttcc aatcgcgatc
    14881 gtgcgggcac agcaccactt tgggctgatg ctgctccagg atgttcttaa tcaccccgac
    14941 gcacgccgcc cagtattgcg ggtcctgctc gcgggttttg ggcgtgatcc gctccagccc
    15001 attgggcgcc gtggtcacca gggtaaagcc aaggtaatga caggccgcct gcagctcgcg
    15061 cagccgctcc tgctgccgct ccttcttgct gcccagcgtg acggcgacgt tgatgaggtt
    15121 catgcgcgcc tggcgcatca aacgcaccgc cagggcgccg acgatgcact catcgtccgg
    15181 gtgcggggca aagaacagcg ccttgggcgc gtggggggct atctccggcc gcgccgcggg
    15241 gggaaaagtg ccgaggggaa gcgcgcggcc ctgcttgagc aggcgcgcat aatcagatac
    15301 gagttgatga taaggagtca tatacatcta ttcacggttg aaggtttgga ggagtgcaac
    15361 aacaaaaagt ccgctccggg aacagccccg gggccgatga tccgctaaaa aactgccgcg
    15421 ccccgcccgg ccgccgcgcg gcgcgcgcgc tggattcccg cccgttcatg cgcaaagccg
    15481 gatggacatg gcacgcgctt tcccgcccgc ccgctacccc accttcggca ggctcgccgc
    15541 cgcgaccgcc tggccgtggc gcttgtcttt ctcatcggga atgtggaacg cgatctggcg
    15601 ggccagctcg gggaactcca ctttcaggac ttcccgggcc ccggccagaa tgacgtcgcc
    15661 gcccggcccc gaggtgaccc ggcccaggat cagcacattc gccaggtcat agaagtccgt
    15721 gaaatgcgca atggcatagc ccaggtaggc gccgatggtc tcataaatct tgcgggcgcg
    15781 gtaatcgccc tggttcatga gctgctgcac gtgcttgagc ttctcgggca gcggcagctt
    15841 cggatccacc tcgatgcccg cgggcgcgag cagccgcccc acgcactgct gcgagaaata
    15901 ctgcaccccg cagccatagt cgccggacca ctcatcggtc ggcgcggccg ggttgagatc
    15961 caccggcaca aaggccagct cgttcagcca cgaggtgatg ttgccctcgg cgttgacgta
    16021 gccgccggcg gtgctggtgc cgagcgaaat ccccaggatg cggttctggc ccagcgacat
    16081 cgaacccgcc agcgccgtga cctcgccgtc gttgaccacc tccagcggaa tctggttcca
    16141 ggccttgcgg atttccagaa acaaatcccg cacccgccgg gtgaacacgt cggcgggcac
    16201 gccccggaaa agcgaggcga ccttgacccg gttgttcaca tacacccccg ccgcgctgcc
    16261 gccgatggcg tccacccgcg gcagatgcgc ggcggctttc ttgagggaat ccatgatgcc
    16321 gtcatagtga tactgcgggt cgggctggaa gtaggggtcc cagaccgtct cttcgctgaa
    16381 caccaccttg ccgtcaatga ccgccgccac cttccggtcg ctgcccccca ggtcaaagcc
    16441 gatgcggcag ccgtccagat gccggcccag cgacgtcgcg ccgtgccgtt ccggcggcaa
    16501 agtctgggca tggaccacct cgatcggctg atcaaacatc cgctcgccca cgagatgcga
    16561 gtcaaacttg ccggtgggcg tctcccggta atgcgccgcc agcttcgccg ccaacgccgc
    16621 cggcccttcc acatgaatgc gaaacccgcc gcgtgaccac agcaggaatt tggcaatgcg
    16681 ctccaggtag gcaaaattgc ccgcggcctg cggatggctg tcgggcagaa tctccgagct
    16741 ataatgggac acggtgccgt cggtctgttc caacgccagc cgaaccatcc ccgaaccggc
    16801 ggtggcccgg gccagcgccc gaaaagcgcg attggccagc accgccgggc ggaaagccgg
    16861 atccaataca ggtgtgactc gtggtgcgat taaaggaagt ccattgttcg tattcatttc
    16921 gcgtgagcgg ttgtaatcca acccaacccc gaacgcaagc caagacagcg cctccaaagg
    16981 cctctcccga ataacccaca ccctcgcacg cccataccgc cccgacagcc tcccgcccgc
    17041 caataccctt tcccatcgca tccccgccag actgtccatt tattggacac tccccccaat
    17101 tcagccctta aaagcccact tccacctcaa actcctcaaa atacccagtc gtcctacgat
    17161 tctcgctaac ttcattcttt caagctactt catggctccc tcctggccgt gtacaagggg
    17221 tgtacaaggg atgctcatcg gatgtactag ctaaaatccc atggacttcc gatgagcatc
    17281 ccttgtacac cccttgtaca cggccaggaa agacgggggg aggacggggg tgggcggggg
    17341 gattgttggc ggctttctct ggatttttgt ggagcgggga cgggttgggg cggggttctt
    17401 tccgggaata gagcctggct agcgtgaaag attgcttgcg gctgcgcgag cgagtctaaa
    17461 gacttccgcg atgacgactc atcgctttaa ccggttggtt ctggggttgc cgcgccgcct
    17521 ggtgctgccg attgcgacgt atccgggcct ggcgttaacg ggggcaacgg tgcggcaggc
    17581 ggtcaatgat gcctcggcgc agtttgaggc ggtcgcggcg cttcacgagc gctaccggac
    17641 gccggtcgtg ttgtccgcga tggacctcag cgccgaagcc gaggctttcg ggtgcgcgct
    17701 gcacctggcg gacaacgaaa tcccctcggt gaccgggcgg ctggtgacga ccctggagca
    17761 agcccgggcg ctgccggtgc cgcagccggg agaccggcgc acggcggtgt acttggagac
    17821 ggtgcggcgg ctgaagcaac tgccgtcgcg gcctttggtg ctgggagggt gcattggccc
    17881 gttttcgctg gcttcgcggc tggtgggggt gagcgaggcg ctggaaatga cgctggcgga
    17941 gccggaactc atggcggtgg tgctggagaa gagcacggcc ttcctggccg cttatgcgcg
    18001 cgctttccgg caggccggag ccgatggctt gatcatggcc gagccggccg cggggctgtt
    18061 gtcgccgcgc ggcgtcgcgg ctcattcctc cgggtatatc cggcatatcg ccgcggcggc
    18121 cggcggcgag gacttcgggt tgatcttgca taattgcgcc gccaaggagc tgcacctggc
    18181 ggccatcctg gagacggggc tgagcgcttt ccacttcggc gcgcccatgg acttgccgtc
    18241 cgcgctgggc aaggtgccgc ccggggtggt gctgtgcggc aacctggatc cggccggcgt
    18301 cttttgccaa ttagcccccg cggaagtgcg ggcgcgcgcc gcggagcttt tatcccgggc
    18361 gggcgctcat cgcaattatg tgctttcctc cgggtgcgac gtgccggcgg ccgcgccgct
    18421 ggccaatctg gacgccctgt acgcggcggc cagcggggct tgagtgtcct gaattgagac
    18481 agggacgcgg agggtgcgcc cgaatcgggg cactccggcc ggccggggcg cggcggcccc
    18541 ggcggtagat gtttttggtg gcctgcggcg ggctaaccgg ccagcagcag gcgcatcaga
    18601 accattgctc ccacaacgaa ggtcgcggcc cacagggctt gacgcattcc aaaccggggt
    18661 tcgcgccggg catccaccgg cgccagcatg gcgctgatga agccgcgatt ggcttcattg
    18721 gctgtataaa tcactttcgg cacaattggc agagagcaaa tcacgcgcca gcgggcggcg
    18781 ggggcagggg gttcgcccgc caccgtgccc gggcgcaaac ggcggtgttc ccggcccggc
    18841 gcggtccctt tcccgggcaa tcaacccggg ccgccgcgga gaggcttgcc tgccgcccgt
    18901 gatgggctag actgcgggat atgaaacgaa gcgcttccat tcattatctc gcggccatcc
    18961 tcatcctcgc cgttggcggc tccggcctgc tcccgctccg ggcgggggcc cagggcgcgg
    19021 ccccggcggc ggcgtccggc gcgttgacgg tggtgtccta caacctgcgg tttgccagcc
    19081 cgactccccc caatgcctgg ccggtgcgcc ggccgttgat ggttgagctg attcaaaagc
    19141 tggcgccgga tgtgatgggg acgcaggagg ggatttatag ccagttgcaa gatctcgccg
    19201 cggacctgcc ggcgtatcag tggattggga tggggcgcga cggcggcagc cgcggcgagt
    19261 tcatggcggt gttttatcgc ccggcgcggc tggagccggt ggcgtttgat catttctggc
    19321 tgtcggacac gccggaaagc atcggctcga agacctgggg cccccggctg gcccgcatgg
    19381 tgacgtgggt gcaattccgg gatcggcagt ccggggagca atttgttttc gtcaacacgc
    19441 atctcgacca cgaggtgcag gcggcccgcg agaaaggcgc ccagctcatc cgcgaccgcg
    19501 tggccgcctt caagcatcaa ctgccggttt tgctggtggg cgatttcaac gccgccgccg
    19561 ggagcaacaa ggcctattcc atactcacgg gcgaccaatt cttcaccgac acctgggtgg
    19621 ccgcgccgaa gcgggtgaat gaagggattg gaaccatgaa tcatttcaag gcgctgcaga
    19681 agaacggggc gcgcattgac tggatcctga gccggggcaa cgtggcggtc gagcgcatcg
    19741 agatcgccac cttctcgcgc gagggccagt ttccgagcga ccactgcccg gtggtggcca
    19801 ccctgcggct gggcgcgcgc cgttagcccg gcgcggttcg gcggccagcc ggggcggcac
    19861 gggaagcggg gatgagtttc gggcggggcg gggttggccc gggcgggggc gggcgcgggc
    19921 gcgggcggcg gcgggcggcg gggctcaggc ggcgagtttc aagtgggaga tgcggaagat
    19981 agcgccgcgc ggttcgtggg gcaggcattc cacctcccag ccatgcgcga gcacgatttg
    20041 ctgcacgacc gcgagcccca ggccggtgcc ttgtttctgg gtcgtgaaat agggtttgaa
    20101 gatttccgcc cggtgcccgg gggggacgcc ggggccgttg tcgcgcactt cgagcgtggc
    20161 ctcggcggca ttgcgttttt cggcggcgat ctggatttcc ccgcggggct cgacggcttg
    20221 aatggcgttg agcagcaggt tgaagagggc ctgccggaag gactgctcgt cggcttcgac
    20281 ggtgaggggc tcgcctttga tctgcaggtt gatctgcttt tcctccaggt cgtaattcag
    20341 ggcgcggacg acttcgttga gcaccgcgtt gagggcgagg cgggtgcggc ggatttcgcg
    20401 cgggcgggag tagttgatga actcgttcaa ctgcgcggcg actttgtcgg cctccgcaat
    20461 gatcgcccgc gagcgttcct gaatctccgc cggggcggcg gtttgccggg agatcatctg
    20521 ggcgaggccg cggatgatgt tgagggggtt gcgggtttcg tgggcgagcc cggcggcggc
    20581 gaggttcatc tccttgaggc gggtgttcat ttcgctggtc cgcaccaggc gaatttgcag
    20641 ctcggaggat ttggccaggt ttcgccaggc cagggcggag ccgaggacgg agagcccggc
    20701 cagcgcgacg atgatggtcc gcagccacaa atcccgcgtg atcgccgccg ccagcccctc
    20761 ggtggacagg gtgagcacca gcccgtggag ggtttgcttg gcgaccaggg attgaaagtc
    20821 ctcctccgcc atgccccgca gccagaaagg ccgccgcggc cggctccccg gctcgcgcgt
    20881 ccggccgggc cggccgccgg gctgaaactc gcccgcatcc ggcgggggcg gggttcgcgg
    20941 cgggagcatc tcttccgggg gcggggggcc ccggcgggtg aactcatggc cgggctcgct
    21001 gccgcggttg gtgaactcgc cggggagcgg caggatcacg gtattggtca cgcccagggc
    21061 gctgaggctg gcgcccaggt ccaccgggtt gacgagcgtg acgctccgcg cgccccagtg
    21121 ctcgccttcc tgcagcaggt cttttcgttg gaggtcaatc ggctggccgg cggaggcgac
    21181 gggctggttg gtggcgttga gcaaaacgat ggagagcagt tcgctggact ggatcagctc
    21241 gttggtgcgg ccgtggatca gttcgttgag gacgggttcc aggcgttcct ggggaatgac
    21301 cccgcggaag cgcatgccgc gaatgatggc gctgagcgtg ttggcgatgt ccttggcgcg
    21361 gttgcgcaag ccggtccggg ccgcctcccg gacgcgcacg tgctcctgcg tctgccagcc
    21421 cagcaccagg acccagaggg ctccgagcag gccatacacc agcaagctgc ggcggctcag
    21481 ttccatcggc gggcggggcg gggcggtttc atgcgcggac gccgggagcg gtcaggaatc
    21541 gccgggctga ggggcgggcg cccgtttgcc gggccgcgga ttcgagcgcg gcagcggttt
    21601 ctgcgcgggc aactggtcca ggaactggtt gagctcctcc tcggtcagcc cttggggcac
    21661 tttggagttt tcccgctcgc gggcggtgag cgcggccagt tccgtgggcg cctgcacgat
    21721 atgcggggtg aggaaaatga gcagttcgtt caggttgctg ctccgttgct gccgtttgaa
    21781 gaggtttccc agcagaggga tgtcgcccag caccgggatt ttggtgtcgg agcgggcttt
    21841 ggtgctgttg atgaggccgc cgatgatgac cgtctggccg tcggcggtga ccaccacggt
    21901 gtcggcggag cggatgtcaa tggtgggggc gttgacgccc gccgagattt gggtggtggc
    21961 cgtgaaattc accgaggaga tttcgggcga gacgatcatc tgcaccaggc cgtcggcggt
    22021 gatgaagggg gtcacattca aaatcacgcc cacgtcccgg taggtgacgg agttgatggt
    22081 attgccgtag ttgtcgtagc ggacgccggt gattaacggc acctgctggc cgacggtgat
    22141 ggtggcgggc tgattgttgc gggcgaggat ggaggggcgg gacagcacct tggcgttgcc
    22201 ggcttgcgca atggcccgca gggtgacctg gtaatcctgc ccgaggattt ggtacagccc
    22261 cgcgccgttc tgggagatcg gcgacgccgc cgagaagctc gaaaacggct ggccgaaccg
    22321 gttgaggttg gtgccgacgg acgtgctcaa gccggacagg ccaaagccgt tgacggcgct
    22381 ggcgtcctgc acggtgccgc tcatattctg gcggccccag ccgccttcca ggccgatgtc
    22441 ggacgcgcgc gtatgcgaga cttcgaggaa gacgactttg atgagcacct ggggcttggg
    22501 gcggtccagg ttggtgagca cccggctgat ttgctgcgcg gtcgcttcgt cggcgatata
    22561 cacgacgcgg cgggtttccg gatcaatcga gaaataggcg tccccgatgg tggtgttgtt
    22621 ggggtactgc cgggaggtgc tgctgccgct catgccgccg cgggcgccgg cgccgcccgg
    22681 ggcgccgccg acaaagccgc tgcgctgctg ggcgtggagc gggaaggcca gggccagggc
    22741 cagggccagg cttccggcca ggcccgctcg ccattggata ttcgtttttc tcataattac
    22801 ctcgtggtgt ggttcgtgct cagggtttcc gtcccgggac gcgggcggct aaaaaccgcc
    22861 gctggtattc cgggtcgcag ccccggtgct gcggacggtc cggccggaag tggcctgcgt
    22921 gttttgcgtc gcccggcggg tcaggggatt gttatccccc aaccgggagt tttggctgct
    22981 gctgctcctg cgcgtattgc ggttgaacag gtcctcgagc gccgcttgca catcctgggg
    23041 gtccgcgttt tgcaggtcat agaatcccac gacctccttg cggcctttgt cggcatccag
    23101 ctccgcgatc atgtcggcga tctgcggcat gagcgtcttg gaggccatca cgatgaggga
    23161 agaggtgcgc gggtcggcca cggccagcac ccggcccatc tttttggcgc ggtcgctgtc
    23221 gctggcgccg ccgccgcggc gggtggcggt gctgccgcgg aaaaagaagg gcatctggga
    23281 gcccgtgctg ccggtgccgg aagcgccggt gggctcgggg aacagcaggg ccagttgctc
    23341 ggagatttcc gcgggatccg cgttggccag gcgaaagacg cgcaactcgg tgacatcggt
    23401 gacctcctgg tcaatttcca tgaccatggt cacgatggtg gccagcaggt ccgcgggcgc
    23461 gctgatcacg accgaattgg agcggtcgtc gcccaccgcc accacctgcg cggcggccgc
    23521 gccgctgccg gggccgctcc ggttgttgct gctgctgccg cggccgcggt cgccaaaccc
    23581 gggcggaccg ccgccgccaa acatgctgaa aaagccgccg cggccgctgg tttggcttcc
    23641 gggcgggggg gcgaatagct gggtaatgag cgtcgcggtt tctttggcgt ccgcgtattt
    23701 gagcggcagc acccggatgg tggaaacgct ggcaatcgag gtgtccaggg cggagatgat
    23761 tttcagcatg cgtttgatgt cggtcttggt cgccaccagg atcagcgagc tggcgctttc
    23821 gttggcggtg agcgtggccg tggacggcag cagcaattcc aggttgggca ccagttggtt
    23881 gacgttggcg tggcgcacgg ggatgatttg ggtcacgacc tcattggatt tttccaccgc
    23941 ctcggggtcg ttgccctgca cgatttcaag gtctttggtt ttggcggagt cctgcgcgac
    24001 aatggtcagg atgcgcccgt tgcggatgac ggcgtagcca tttttcttga gggccgagtt
    24061 cagcacttcg accgcttcct cccgggtcag atccttgccc tggacatcca gcgtgccgcg
    24121 cacgtccgcc tccttgttga tgataaagcc ggcggcgtcg ctcaggtagt ccaggaccag
    24181 gttaagcggg gcgttatgga agttgagccg caggccgttg gtgccttgct caatccgcac
    24241 tggcggttcc agcgcggccg ctgcggcggc cacgccgttg gtgccggcgg ccggggcgcc
    24301 ggcgttctcc gcgggggaag cgggcgcggc cggttgcggg ctggcggggg cggccttggt
    24361 gtccgccagg gccgccgggg gggcgttggt tggggtctcg tccggcgcgg gcgccgctcc
    24421 ggcggcgcaa acgcccgcgg ttaagccggc aaacaacaac aggtggtgaa tggtcttcat
    24481 tgtcaattat tcctccagac gtttttgcat cagtcgtttg atgatgtcgc tctccgcgtc
    24541 gctggcggcg ggggccgcgt ccggggcggc cgcggtcgcg gtggtgttgg tcgcggtgga
    24601 agcggccggg gtggagttgg cttccgccgg gctggcgagc cgccagggcc cgccatcttc
    24661 gcggcgcaac tgcgttccca ctttcaattc cagccggttg gtttccccgg ccagcgccac
    24721 attgtcgggc gcgatgccgg ccacctggta gccggcaatc gtgtcgcgca gttgcaacgc
    24781 cttccggaac tccgaactgc ttccgtcaaa aaaggcgaac cagcccttcg cataactcat
    24841 ggtgccgacc agcgtcacgg tatcaatgac cttgggcgcg ggccgaaccc ggggctcccc
    24901 cgcccggtgc gcgtagcggt tggggtcaaa gatattccgg tccgcaatga ttttgaacgc
    24961 ggagaagtca aggctggccg ggcggctgtt ggttcccgcg ggtgcggcgg ccgggcggcg
    25021 gtcgccttcc gggctggggt cgccggccgg gcgtgaactg ctgcgcgtgt tatcactggg
    25081 ccgcgcggaa cggcggccgc ggaaatggcg tgcgtccttc tgcgatcggc tggaaccggg
    25141 gaaacggttc gtgccgttcg tggccgccgc tcccgtggcg gggcggctgg cgcccggggc
    25201 cgccgggctg gccggggaaa cctcattggt ggcgctgagg ttttcgggcc gctccagatt
    25261 atcaaactgg ccggattcga cggcttgaag cagctcgcca aggcgggcca gcatctcgga
    25321 gtcgctgagg tcattcgtat ggttttccgc ggccggttcc tgggcggcgg ccggcggccc
    25381 gctgaccagg aggaacgcca ggaggatccc gcccgccgcc cggccggcgg cccgccgcca
    25441 tcggcagcgc ctggcactca ttcgttcggt cagcaagctc atacgcggca gcgggttcat
    25501 ggtttctgcg gagtgaggac cagggcgctg agttgcaggc ccagcgaaag ctgctggcct
    25561 tccttgtcgc gcgcgcccag ttccaccgat tcgagtttca gcgccatcgg ctcccgctcg
    25621 acgctgtaca gaaagcggct gagcttgccc aggtcgccag caacgtccac gcggcattca
    25681 aaggtcttgt aatcgtcgct ctcctgtttc cactggggcg tgatggccgc gatcgtcacc
    25741 ccgctgtttt gcgcccagag atcaattgcc ttgaagacct gctgctcggc cgccgaggtg
    25801 ttggcgggca gggtgttttc ggatatttgc tgccagcggc tgcggatgct tggctcgcgc
    25861 tgctggagca agcggccgtc ggtaatttgc ttctgcaatt cggtgaggcg ggccgcccgc
    25921 tggctccagg ctttcaggag cggggacagc acgagccggt ctcccgccaa aagggcgagg
    25981 gcggccccgg cggcgatgag gaggaattgc tggcggtttt taatcggcat tttggcctcc
    26041 ctcattccac tggaagttga acgtgaattg cagcgcgggc gattggcccc gggttggtcc
    26101 cagggccacc tcgcggaatt ggcggacctt gcgcaggttt tcgaccgtct tcagcagcgc
    26161 cgggtagttg cgcgccgtgc cggtgcaggt cacgttgttt aagccccgga tctccacggt
    26221 cttggccgtc acggcgccgt cctcggggaa ggcttcggta agatgccgca ggatggtcag
    26281 gctgcgcacc gagttgtcgt gccagggccg ataacgccgg atttgttcct gcaaatcttc
    26341 caactggcgc accgccgcct gcatcccgcg ccactgcgat tccaggcgcc agagccggac
    26401 ctgctggtaa aggaacaacc cgcccaccac cagggccgcc gccgccgccg ccatggccac
    26461 cgtgcgcagc ttgcccgaag aatagcgcgc cgcccattgc tgccagggcg tcggcgtggg
    26521 cagcagaaac tcgaatcccg cgcggcgttc ggccagccgt tccgccgcca ggctgaaggc
    26581 gggcgagacc ggcgtatccg ggggcgccgg gctgccgaat tcgttggcgg cataacgttc
    26641 cacggactcc acttccaggc cggccgcctc cagccgcaat tccaactcat ccgccaattg
    26701 gcggctcagg tgggtcgggc caaatacccg gatgcgccgc acggtttccc gcaggcccgg
    26761 cggcaattgc cccagggtga tgcggacttc gcgggccacg acatccgccc ggagcagccg
    26821 ctggcttccc tcgacttcga gcgcgccttc cagcgcccgc agcgctgcca ccccgccgcc
    26881 ggccgtaagc tgcaatccca catggctttc gctgatcgcc agcgccagca cgccgtccgc
    26941 cgccgcgagg ccggggggtt gcaaggccgc gacgcccagc gcgaagctca gcggctttaa
    27001 ctttgctgcg cgcagcgccc gctccagggc ggtcaggtgg tttttcggaa tgccgatcaa
    27061 cagggcgtgt tgcttgccgt cggcggtctg gaagcgcgac gtcgcgatgt ggagcgtctg
    27121 gacatcgcag ggaaaccccc gttcggcttc aatctgcagc aggctggcaa cgtccgcctc
    27181 cggcagttcc ggcacttcga cctgggtcgt aagcgcccac ttgagcggca gggccaccac
    27241 gcaggggcgc tcccgaatcc cctgggcctc cagatgattg cgtatttccc ggcccaccaa
    27301 ttcggggtca ttggtcagcg ggtcaagcga cagcgagacg gagaacgccc cgtgaacgtg
    27361 cagggccccg ttggcgcgcc gcagggccag gccctcgagg cggctgccgt ccagggccag
    27421 ccccagcacc ccggaagggc gttggcgccg gaaccagtct ggtcggttca atttaatcat
    27481 gttgtgtccc cgctaagagc cagtcctggc gcacctccgg ccccagggcc cagccgagat
    27541 gcgtcaggtc ctgccggtaa acgatcacgg gactgccgct gctggtgtcg aaaacgaatt
    27601 ttacgcgccg gtatccgcgg ccaaatggcc ccagggccgc cacatccgca gtgaattggt
    27661 agctttgcgt ggtgagcaca tcgcgggcgg ccagggtttg cagggcttcc gggtaattct
    27721 gccgcaaggc gtccgccacc caggcgatcg aggtcagttc gtccggattg gattgccgat
    27781 agttgaccag catttccgcc accgcgacat ccccctccag caagcaggcc agcaccgccg
    27841 cgctggcggt gttcacgttg acccggcccg tgacgtacgc cccctcggtt gcggtcaggt
    27901 tcgtgctgat gagcgcaaac tccgccgccg tcatgccgct gcccaggtaa aacgcgagcg
    27961 ggctggtgaa gcgcgtcccc gtggcgccgc cgccgggcgc gcgccccggg cggctgcccg
    28021 ccaggccgac cgttgccagg atttgatcgg cgcgcgccgc gccaaaattc gtggccagca
    28081 gcgagccgag ctgcgcggcg aagccgttga ggttggcgac gttgatgcgg gcggacccat
    28141 cgcgctgggt attcggctcg cggctataga cggtgcaata ctccagcacc ccgggatcga
    28201 gcaggttatt ctggttgtca tcggtttcag cggcatccag gacgccattg cgattcgcgt
    28261 cttcgcccag cagggtgtcc atgtcggcgc cgtagagcag gcgcaactcg tccaccgtct
    28321 caaagagatc gcacttgcac tggtaggcgg gctgctgcat ggcgtagtag atgacggtgg
    28381 ggccattgcc gttggtgtcc cgccaatcga ggatggcctg ggtcaggtct tcggtcatgc
    28441 ggggcagcca gatcagggtg tcgccctccg cggaattaag gttgagtttc gcggcttcgt
    28501 ccaccaggcc aaagcataat tggcccgggc cgatcgggtt gttggtatcg cggctaatca
    28561 gccaatagtg ggcttccccg accgggaccg cctggcacag cggctcctcc aggctcggca
    28621 gcacgccgtt ggagccattg ctgatttgcg ccgccagcag gtagttcagg tagcgaaccg
    28681 cgccttcaat tgcctggtcc gccgcttgcg ccgacacccg gttgtcggcg gcgcgcagtt
    28741 caaagctcat ggcgttggcg aaatacaacg ccaagcttac caggccgaag gctatccaca
    28801 acacgatcac cagcaccgaa ccggcgcggc agcggcgcgc ggaccgggcg aacccggagg
    28861 ccggagattt ttcccgcagc ctcataagga ctcctccgcc atcgcgcggg cgtgagtttg
    28921 cggcgccagc gggacgacca tctcaatcgg agacggttcg cctcccgcct ggcccgggcg
    28981 ggacgcaagc tggatgcgga cgcgcacggc cactggcagg ttggtgtcgg tgttgctggt
    29041 atcccaggtg tcgcgccatt gcgcgccatc ataacaatca aactccagcg tttgcacccc
    29101 gctcatcagc cgctggatgt ccggggccag aatggtggtg gccaacaaat tgcgatgcac
    29161 gcagcgcacc aggtccatgc cggcctgctc ccggttcgcg ggggccttca attcatagaa
    29221 aacctcctgc acatcgcccc agggggcgcg gtcggaaagg gtgccggttg tggtgtagaa
    29281 atccagcccc gcgctgccgg cttcgccgct caagcccatc gtggccccga ccgccggcac
    29341 cccacaccgg aaatcgcccg ccagcgacct gcccgggttg accgttcctt tcaaatcctg
    29401 gtatagcagg gccagcgccc gattaaccgg cagcgattct tcgagggcgc cggaggtatt
    29461 gtcccgcagg cgcagggcgg tggcaaacac cccgttgatg gccaccagga caatggcgca
    29521 aatggccagc gccagcagca tctcgatgag cgtaaaggcc tgggcgccgg agcgcgaaac
    29581 cgcttttccc gtccggacgg cggcgatgcg ccccgggcgc ggggcggtct tcagtttcag
    29641 atttcgcgtc cggttcatga gccgtttgtc taaggcagcg tgctgtccgc cagcgtgctc
    29701 aagcgcacgg aatagtcgcg gttgcgcgcc gtgtaggtca cctccaccgt cagcaggcgc
    29761 atcgcctcct ggttggggtc ctgatcccag ggatcattct ggagagtcca ccggaactgg
    29821 cgctgcgcct ccgtcagggt gccgctttga accgactggt tccagttggt ggccagcagg
    29881 ctctcggcca gcagccgttg cgccacgcgg acggcttcgc ctttgcgctc cgccactgtc
    29941 cccgccaggc tggcgatgtg caaccccccg agcgcggtcg gaatcacgat cgccatgaac
    30001 agcagcgccg ccagcatctc cgccagcgtg aacgctgcct cggatagcct tttcgcgccg
    30061 gcgagccgcc ggcggctagt catgggcgct gcggatttca tagttcaatc catcccgcga
    30121 ctgcgccagc caaagcgagc cgccgtcgtg gctggtcagt tgcaatttca ccgggctggt
    30181 gtcgccgacc gtcccgtccg gcagaaagcg aatcgccggc agatgggccc gggcgagcgc
    30241 cacgcgcggc gcgctggccc ggcgggtttc ccgggaatgc ggcagcgcgc tggccggagc
    30301 cgcggccgcc gtcccgccga tcaccttgat ttgcaaatcg ctgtcgaggg caaactccac
    30361 ggctttggga tcgctgggtt cataactggg ttccgcggcc agcccaaatt cccccgccgc
    30421 cgcatccacc cataaatcca tcggcactcc ctcggatacc gcgcggctct ggccgctgcg
    30481 cgtcagcgcc agcaaccggc gcgcttccga atctagcgtc cggccatgga aaaagcgtga
    30541 gagcttcggc gcggtcaacg cgaccaccac cgtcagcagg gccatcacca ggatcaactc
    30601 gataagcgtg aacccgtttg gccgccggcg cccgtttccg cccgcgcccg cgtcggcgcg
    30661 catgcgcgcc cggttgtctg gctgcgcgtt caccgtttga gggtccagtt gccgatcacc
    30721 atgttttcgc ccggcggccc cggagagctc aaatcatacg aggtgggatt atgccgtccc
    30781 gggcattcgt agatgtaatc gttgccccac gggtctttgg gaagggcatc ggcctggaga
    30841 tacggcccgc gccagttctg ggcatcgcgc ggctgctgga tcaaatccat cagcccgttc
    30901 cggccccggg gatagtagcc attgtccact tcgtaggctt ccagggccgt tttgaaggtg
    30961 gcgatctggg tttgcgtcgc ggcgatctgg gcctgccggg tgcgattcgt aaaccggggc
    31021 agcacaatgg ccgccaggat gcccaggatc accagcacca acagcaattc caccagcgtg
    31081 aagccccgcg ctcgcccgcc ggccgttcgg ttcttgttta atgggttgag tctcataagt
    31141 cagcttcgtc gtaggtcatt taatgtagtc ttgcagcgta aagatcggga gcaccatgct
    31201 gataaagatc gtgccgatga acgcagcgat gaaaaacagc atcagcggct ccatcagcgc
    31261 caccgccgtc ttcaattccc gatccaggtc gccctcggtg acattggcga tccgcaccag
    31321 ctcctgatcc agccggccgc tctcctcggc caccgaaatc atttccacca ccgagccggg
    31381 gaacaggccg cgcgtctccg ccaggctcgt ccccaactcc ccgccctgct gcacgcgctc
    31441 gatggattgc gcgaccgcgt ccaccagaat ctggttgccg atggacttgc gcgccacgtt
    31501 cagcccctgc accagcggca ccccggcgcc gagcaacgtg cccagcatcc ggcaaaaccg
    31561 cgccatggca aactgggcca ccagcggccc caccaccggc gaccgcagca ccagcccctc
    31621 ccacacgcgc cggcctttct ccgaggcaaa ccaggtccgc agcatgaacg ccgccactcc
    31681 caatcccacg gacacgaaca gcccgtagcc ccgcaccagg ttgctcaccc cgatcaccac
    31741 ctgcgtcgcc aggggcagcg cggcattaaa ccccttgaag atgccctgaa agcgcggaat
    31801 gaagaacgtg aggagaaaaa tcaacacccc gatcgccagc acaaacagga tgctcggata
    31861 aagcatcgcc gacagcacct tggagcgcag ttccttctcg cgcgcctgaa agtcggcgat
    31921 ctgcgccagc accaggtcca ggaacccgcc cgtctccccc gcctgcacca tcgcggtata
    31981 aacccgcgga aacgtctccg gcgacttccc catcgcatcc gccaaagaca tgccgtccac
    32041 gaccaggtca tggatttccc tccacttggc gttggcggcg ggcgaggaag cctcccggta
    32101 aagaatcacc agcgcgcggc tcagcggcac cccggcggcc agcaaactcg agagcagccg
    32161 cgtgaagttc tccagttccc ggctcgaaac ccgggctgac cgcttgccga acgacaacgc
    32221 ggccagcgag gccggcagcg caaccccggt gtccgcgccc gctttttccg caaggctgac
    32281 cgggcgcagc cccaactcga ccatttgcgc gaaggcctcc gcccggcccg cggcttcgag
    32341 ttcgccctgg gtcatcgcgc cgttggcctg caaggctttg tagtgaaaga acggcatggc
    32401 cgcatccgtt acggcgcgga ccgggccgcc ggttccttgc ccgcccccgc cagcgccgtc
    32461 ttttgcgtgg tgcgctcaat ctctttggcg gtggtcacgc tgagcacctc ctcgacggtc
    32521 gtgcgcccca ggcgaaccag gcgccagccg tcctcggcca gggtttgcat gcccgcgcgg
    32581 cgggcggcat cccggatcgc gtcgctcgag gcattcttga ggatcaactg ccgaatctcg
    32641 tcgtcgctgt ccatccactc gaaaatggcg tggcggccgt agaagccggt ttggcggcac
    32701 gccgggcagc cgaccgaacg ataaatcacg gtggcgaggg ggatgcccac ctgccccttg
    32761 aacgcccggg ccgccgggga atcgtcaggc tgcttgcacc gttcgcacag cacgcgcacc
    32821 agccgctgcg ccagcacggc ttcgagcgac gaagccacca ggtacggctc cacgcccata
    32881 tccaccagcc gcgtcagggc ccccggcgca tcattcgtat gcagggtcga aaacaccagg
    32941 tgccccgtca gcgaagcctg caccgcgatc tgcgccgtct cctggtcgcg gatttcgcca
    33001 atcagaatca cgtccgggtc gtggcgcagt atcgagcgca gcccccgcgc gaacgtcaag
    33061 cccgccttct ccgaaacctg tatctggttg acgccgcgca gttgatactc caccgggtct
    33121 tcgatggtaa tgattttgcg gtccgcgtca ttgatctcgt tgagcgcggt gtagagggtc
    33181 gaggtcttgc cgctgccggt ggggccggtg accaggatga tgccgtgcgg cagttgcagc
    33241 acgcgccgga agcagcccaa ttcacgctcg gccatgccca gctcgcccat cccgcgcagg
    33301 gttgcgttct gccgcagcaa ccgcagcacc agcgcctcgc catgcagcat cggaatcacc
    33361 gaaacccgga tgtccacctc cacctcctcg atccggacct tgatgcgccc atcctgcggc
    33421 agccgctttt cagcaatgtt caagtggctg agaatcttga cccgggaaac aatcgccggc
    33481 tggaaccgct tgatctgcgc cggcaccggc acgtcctgca gcacgccgtc aatccggtag
    33541 cgaatgcgga actcctcctc gaacggctcc aaatggatgt ccgaagcccg cagctcgatc
    33601 gcatcccgca gcacctggtt gacgaagcgg atgatggacg cctcctccgc ggcggcgtcc
    33661 aggtcggtgt cctcctcgtg gcgctcatcc accaccatga aggacgcttc ctcgttcagc
    33721 gtgcccagcg tatccgcgcc cacccccagc cgctttttca tttcccgctg gatggcttca
    33781 ctggcggcca gcaccggctt gagcggcaac ccggtcaggg tcttgagcgt atccagcccc
    33841 tgcgttgcga acaggcggct ggtggccact tccacggcgc cattgctgcg ccgcagcggc
    33901 aacaacccct cttttaacag aatccgcgcg ggaaacagcg atagcaactc gcgatccggc
    33961 tcgacatcct ccagggtcgt atacgccagg tcgtattccc gcgccagcca gcggagcaca
    34021 tccgcctcct cgtgcagggg cgcctgcgtg ccgcccgggt tttgccgcag cagggtcgcc
    34081 gcgtcagccg ccgccagttg gcgggcggcc accatccggt cgagcaactg cttccaggcg
    34141 gcttcctggg ctgccaattc actcatgggc gaaccttcgc cgcgggggcc ggaggcggaa
    34201 gcgcggggaa ggggcaaacc gtctttccgc ggaccgcgct tcgcgccggg cgcccgctca
    34261 cagcaagccg ttgagcaccg cgatggcttc ctgccgcggc gtcagcagct cgcgcagggc
    34321 cgcctgggcc ttctccatct ccgcctgttt ggctttgcgg gaagcggcgt atttctccaa
    34381 cgccgccttg agctccgcct tcggcgcctt ggcgtcaatc gccttctgca aggcctccgc
    34441 ttccgggctg ggcgcgccaa agaaccctcc gcgccgcgcc gcgcctggat cgctcggcgg
    34501 cgcgccgtcg cccggccgcg gcccgcggcc catcatcccg cggcccaggc cgctgaaagc
    34561 ttcccgctgc aaatcccgca ccttttgcac gcgcggttga atcgccttcc attcctcgtc
    34621 gctggtcacc tccagcattt cccgggtgcg ttccatcatg cgttgctgga attgcgccgg
    34681 atcaaaattg ccgcgccccg gccggccgcc gggaggagcg ccgcccggct gcgccacgac
    34741 ttgatcggct cccaggctca tcaccgccgc cagccccgcc atcactaacc attgattcat
    34801 tttcatactt atttctttta cggtttggtt gtatgcggcg ccaaaacgcg cgtccgcgcc
    34861 gtctcgagcc gcacgatgcc gtggtatatt gcagatggcg tgccaagcgc ccaaggcctt
    34921 ggatttcgca ggtttagccg tggcgctgcg caagctttgc tcgcttgcgt cccgccactg
    34981 cgcagaggtt gcgcagtcca acgaaacggc ggagatagcc cggggccacg cgctacgcgc
    35041 gccgccgcca ttcggttttc ggcggctcgg gcaacccctg ggcgcgatag gcggtgcgga
    35101 agtgcatctt cgccaccttg gccagccctt cggcgcccag ccggtcaacg atctcccgcg
    35161 ccgccgcatc caccgcggcc gaagcgccct tgggcaattt ccggccgcac tgtttctcca
    35221 gcgcgcccag gcgggtgacg aattcatgcc gggccaggat cgaagccgcc gccacggtga
    35281 ggtcttcctc ggcccgatgt ttctgcacca gctcaatctc ccgccccaac cccatcagcg
    35341 cccgggccac ggtttccttg ctgtgggcga actggtcgct gatggcccgc accggcggag
    35401 gattcatccg ctgccgctgc tgcatcaggt tctcaatcac ccgcgcgtgc ccccaggcca
    35461 gaatggcgtt gacgctgccc atcttcccat acagccggtt gtaggcttcg ttgccaatgg
    35521 gcaccaccgt cgtgacgccg ccgggcgttt tgcgaatcag ttccgccagg tccttgatgc
    35581 gcttttcact ggtgatgttc ttcgagtcgc gcacccccgc ctccgcccag gccttgacga
    35641 cgctcgcgtt ggcgtaggcg ccggcaatgc aaagcggccc gaagaaatcg cctttgccgc
    35701 tttcgtccac ccccagccgc ggcagcagca gatcgggatt cagcaccgcc tcatatccca
    35761 gccgcgcctc cttgagaatc tccggctcca gcacaaactc gacaaactct tgcgtgccct
    35821 tgccctgcac caccagcttg ccgctctcgt agaaaaccac gctggtcttc tccttgtccg
    35881 ccgcaaaccg cgcatacggc accggccgaa actggtaaaa atgcgcctgc agccagctcc
    35941 ggagggcggc cgcctgcgcc tcggtgagct tgccggtata ggaggtcaac ggtttcacac
    36001 gaatgccggc atcgtaaagc gacggcggcg gcagggcaag ccagccgtaa gccaaaggac
    36061 gcccccggcg cggcgcgcaa cttcccgcgc cgcgcttctt tcgcatttcc gattccggcc
    36121 ttccgcctta ggcttcggca tcgcgatgaa gaatggcgac atcgtccaaa agctgctggc
    36181 gtggttcgcg cgccacgcgc gcgacctgcc ctggcgccgc acgcgcgacc cctacgccat
    36241 ctgggtttcc gaaatcatgc tccagcaaac ccaggtcaaa accgtcctgc cctattggcg
    36301 gcgctggctg cgggcgctgc cccatctcgc cgccctggcg gacgccccgc ccgccaccct
    36361 gcacaaactc tgggaggggc tcggctatta cgcccgggtg cgcaacctgc agcgcgcggc
    36421 gcaaatcatc cagcaacaac accacggccg cttcccgcgc gacttcgacg cggtgcgggc
    36481 gctgccgggc atcgggcgct acaccgccgg ggccatctgc agcatcgctt tcaaccagcc
    36541 gcggcccgtc ctggatggca acgtgatgcg ggtgctggcg cggctttatg gcgtcgccgg
    36601 aaacccgcgc gcgggccggg ccaacgcgcg attgtgggag ctggcggaag ccctggtgcg
    36661 gcgcgcggcc gggctccggg cgcgccgcgg ctgcgcggcg ttgaaccagt cgctgatgga
    36721 actgggggcg ctcgtatgcg cgccgcgcca gccccggtgc gcgctatgcc cggtgtccgg
    36781 cgcgtgcgtg gcgcaccgca cgggccgggt ggcggaactg ccggagcgcc cgcggcgaac
    36841 ccggccgacg ccgcggcggt tcatggcgtt tgtggtggag cggcacggac gggttctcgt
    36901 gcggcaacgc cccgccgggg gggtgaacgc gcgcctgtgg gagttcccca atgtcgaggt
    36961 cgcgcccgga gattccgatg cgcgggcggc ggcgcgcaaa gctctcggga gattgccggc
    37021 ggcgctggaa ccgctggccg tcctcccaca caccatcacg cgctaccgca tcacgctggc
    37081 ggcatatcgg gcggcctggc gcggtgaaac caccccctcc cgggccggcg cccggtgggt
    37141 ggagcggcgc gccttgaacc aactggcctt tgccggcgcc caccggaaga ttctgcggca
    37201 actccaggcc gggccaggca aaccgtctta acggcccgcg ccggaaccgg gaacccggca
    37261 tttgccggcc cggaagcggc cagcccggcg cgattccctg cttgatcccg gcgaacgccc
    37321 ggctcatgct gcgggttgta tgaagttcct cattctgctt tcgttggtgc tgtgcctgcc
    37381 cggcgccgga tccagccgcg ccgatgaaac caacgcgccc gcccttaccc tcagcgccgc
    37441 ccaggccggc gaatacatcg ggaagcaggc cacggtgacc ggcgttgtgg cccaggtcag
    37501 catccggccc agcatcacct tcctgaactt tgacaagaaa taccccgcca gccccttcgc
    37561 cgcgatcatt cgcagccgcc ataccaatca attcgagaat ctgcccaacc tcagcggcaa
    37621 ggccgtcgcc gtcagcggca aaatcattga ctacaacggc aaagccgaaa tggagctgac
    37681 ccgcaaaacg caactcaagc tcctcaccaa cgccccgccg cccgccccgg cgccgccggc
    37741 gccttgaata tccaccggcg cggccgcgtc gtgcctggag caaacaaacg cttggcccgg
    37801 cgcgcaaccg gcgctatcgt gtccgcggaa tcagaatggc cgggccgggc gcgaccagcc
    37861 ccggcccaca acccgccaga gtaacttcaa cgctttatta tgatgactga gatgcaaccg
    37921 ggttcaacct ccaggaaacc gcgccgctgg ctccgggcgc tggcctgggg cggcggccta
    37981 ttggccgccc tgctcctcct cgtttatttc atcgccacca gctccggctt cttcacgcgc
    38041 gtgattctgc cccgcgtcag ccgcgcgatg aatgcccaaa tcactgtgcg caaggcggaa
    38101 atccgccctt tccggcaggt cgttctgcac gatctcaaag tgcagaccac cggggcggag
    38161 ccgctggcca ccgtggccga agcgcgcctg cgctaccacc tcctggccat cctcggcggc
    38221 aatttccgcg tggatgaact cacgctggct tcgcccacga tcaccgtggc cgaaaacccc
    38281 gatggcacca gcaacctgga cccgctgtgg gcaacgcccg ccgccccccc ggtcgaaaaa
    38341 tcgccgccgc ccgcccccgc gcccgcccgc ccgctccggg tggacctgaa acagctcgcc
    38401 ctccgggacg ccacctttcg ccaggtcaaa aaccacgccc agggcccgcc cgacctcctc
    38461 gaactcgccc gggtcaacgt caccgtggag gatttgaaaa acggccagcc gggccggctg
    38521 accctggcgg gcgacctgcg cctgaccggc tccaacgccc tgctgcacgc gcaactggag
    38581 ggccgctatg acttcgccct ggcggcggat ttacagccgg cttccctcaa gggcagcgcc
    38641 cgactggacg tccgccgcgc cgaaaacgcc ctggccggct tcgccggggt ccacggcgcg
    38701 ctcgacgcgg aagtcacgcc caccgaggtc aaagaagcag ccctgcgcat ccataaaaac
    38761 gacgtgtccc tgggcgaact gcgcgccagc ggccccctcg cgctcgaagc gctcgaaggc
    38821 cgcctggacc tctccctgac cggcatagat caccagctcc tgaacctgtt cggcgcgccg
    38881 cacggcctgg acttcggccc caccaccctc cgctcgacgc atcaaatcga gttgtcccgc
    38941 ggcggcacga acatcaccgc ccgcggccag gtggaggtca gccgcttcca ggtgacccgc
    39001 gccggccaga ccacgcccca attcgatctt caccaggaat acgacctcac cctggacttc
    39061 gggcaaaaga ccgccctggc gcgccaactg gcgctgaccg gcacccagaa cggccgcccg
    39121 ctgctccagg gcgacctcac ccacccgatg cccctctcct ggggagacac ccacagttcc
    39181 ctgggcgact cgacgttcag cttcacgctc acggacctca acctggccga ctggaaaccg
    39241 ttcctggacg aagtggccac cgcgggccgg gtgaccgccc gcgcccagat cctctcgcaa
    39301 cagggcggcc ggcaaatcac gttcaacctc gactcccaaa ttgaccacct ggcaacccag
    39361 gtcggcggcc agacgctcgc ggaagccgcg gtgaccttgc aggccagcgg ccaggccacc
    39421 gacctcaacc aattcaacct cgccagcttc aaactcgaaa tcgcgcaccg caaccagatg
    39481 ctggccgcgg cgtccggctc cggcgtctgc gatttggcgg ccgaaaccgc cgatctgaaa
    39541 cttgccgccc gggcgatgct ggccccgctg ctccaggccc tgccgcaacc ggaaatcaac
    39601 ctcgcctccg gcacgctcga gctcaacgcc cgcctggccc ggcagccgca gtcgcaaacg
    39661 ctcgccggcc aggccaccct cgccgacctc accgcccaat ggggcgaaaa cgccgtccgc
    39721 cacctgggcg cccgggcgga tttcgacgtg ggcctgaccc cgcgggaaat ccaagtccgc
    39781 cagctcgccg gcacgctgac gcaggacgcc cgggacggcg ggcgcttcga cctggccgcg
    39841 aagtatcacc gggaaaaaga gaccgttgag ctgaccgccc ggttgacgga tttcaaccag
    39901 cacggcgcgg gccccttcct cgaaccgttg ctggcgaatc aaaagctggt gtccgtggac
    39961 atcaacgccc aggccaccgc ccaatacgat ccccgcggcg cgtccgccgt aaaggcggac
    40021 ctgcaagtgg ccaacctcgt cgtcgccgac ccgaaacagc cggcccccgg cgctccgctg
    40081 gcggccacga tgcgcctgga cgcctccgcg cgccagcaaa aagccgaggt gcgccagttc
    40141 cagttggcgc tcacgcccac cgcgcgcgcg accaaccagc tccagctcac cggctgggtg
    40201 gactggtccc gcaccaacgc cctccagggc gacctcaaac tgacggccga ggcgctggac
    40261 ctgacccgct actacgacct tttcatggcc gacaccccgg ccgccgcgcg cgaaaccccc
    40321 gcggccccgg acgcgtccgc gccggcggcg gccagcccgg aacccgaggc cctgacgctg
    40381 cccttccacc acttcaccgc ccacgcggcc atctcccggt tgtacctgcg cgaactggaa
    40441 atcgccgacg cccaggccac cgtccggatt gacggcggcc gggtcgtggt caacccatgc
    40501 caactcacgc tcaacggcgc gcccgccgac gccaccgctg acctggacct cggcgtgccc
    40561 ggctggaaat acgccctggc gttcaacgcg caagccctcc cgctgccgcc gctgatcaac
    40621 tccttccagc cggacctcaa aggccagctc agcggcacgg caagcgccca gggcaacctc
    40681 aacggcacgg gcttcaccgg cgccagcctc caaaagcacc tcgccggcca gttcgatatg
    40741 accctcacca atctcaactt cgcgctcgtc agcctcgaag gcaaaacctg gtacacccgc
    40801 gtcctcaaaa ccgtggtcaa cgtcatcggc accctccccg agctggccca aaaccccgcg
    40861 ggcaccgtcc tgtccctgct cacgggcaaa ggcagcgcca gcggcggcgg cgctcccgag
    40921 ctgaagcaat cccgcctgga agcggtcgcc ctgcgcgccg cggccggcgg cgggcgtgtg
    40981 gatgtgcaaa acgccaccct gcaaggctcc gcctttgcgg cccaggccgc cggaaccatt
    41041 cagttggccg aggtcctcac caattcgtcg ctgcacctgc ccgtcgcgat ttccctcgaa
    41101 cgaaccctcg cccagcgcct tggcctggcc ggcgacaccc ccaccaacgc ggtgtatgcc
    41161 cggctgcccg acttcctgac ggtgcagggc accctcggca actccaaagc cagcgtcaac
    41221 aaagccgccc tgctcggcgg cgtgttgcag gcggtggaca gccggaaaag cccgcccgcc
    41281 gcgggcgggg acaccaactc ccccgccgcc ccccggcaac ccggcctcga cctcggccgc
    41341 ctcctgcaag gccgcggcgc ccgcccggac ccgggcgcgc ccgccgccac caacgcgccc
    41401 gccaccaatc ccgcgcccgt caacgaactg ctgcggggcc tcttcggccc agacaaaaaa
    41461 taggcgcccg ccttccgcgc cccgggcgcg gccgccccgc cgccggccgg ccataaaaaa
    41521 accgccccgg ccaaccgggg cggcgtgtca ttgggggacc ggcgcgccgc gtttgcgcgg
    41581 cgcggctccg gctacttctt tttgcccttc cgggcgccct tggccttggc ttccgcctct
    41641 tcctccaggg ccgcctgcgc cgcggccagc cgcgccaccg gcacccggaa cggagagcag
    41701 ctcacgtagg tcagcccgat gcgatggcag aacttcacgc tgttcgggtc gccgccgtgt
    41761 tcgccgcaga tgcccagctt gatgtccggc cgggtctggc ggccgcgctg gaccgctatc
    41821 ttcatcaact cgcccacgcc gacctggtcc accgtggcga acggattggc cttcaccacc
    41881 tccgcctccg tgtaggccgg gatgaacgtg ccggaatcgt cgcggctcat gcccaggcag
    41941 gtctgggtca ggtcattggt cccgaagctg aagaactccg ccgtctgcgc aatctcatcg
    42001 gccgtcagcg ccccgcgcgg aatctcgatc atcgtgccga ccaggtactg gatcttcacc
    42061 ttccgctcgg ccatgacttc ccgggcgacg cggtgaacca cctcggcctg caaatcgagc
    42121 tcccgcttga acccgaccag cgggatcatg atttcgggct tcgccttgag gccggctttc
    42181 tgcacctcgg ccgcggcttc aaacacggcg cgggcctgca tctccgagat ttccgggtac
    42241 accaccccga ggcggcagcc ccggaagccc agcatcgggt tgaactcatg cagctcctga
    42301 acgcgctgct cgattttctc caccggcacg cccatcttgg cggccaggtc ccgctgcgcg
    42361 gcttcctcgt gcggaatgaa ctcatgcagc ggcggatcca ggaagcgaat ggtggccggg
    42421 aaccccttga gcgccttgaa gatgcccaca aagtcggccc gctgatacgg cagcagcttg
    42481 gccagggcct tcttgcgctc ctccacggtg tcggcgagaa tcatctcgcg catcgcgtca
    42541 atccggtcgc cctcgaagaa catgtgctcg gtgcgcgtca ggccaatgcc cgtcgcgcca
    42601 aacgccacgg cgttctcggt ctgctccggc gtgtccgcgt tggtgcgcac ctgcaggcgc
    42661 gtcaccttgg cgcaccaatc catcagcgtc ttgtacatcc ggtacgtctc gctgtccttg
    42721 cccgcgaggg tctgctcgat cagcacctgc acgatctccg acgcggccgt cttgatttcc
    42781 ccggcgtaca cgtggcccag cgtgccgttg atggacaggt agtcgccctc cttccaggtc
    42841 tggccgttga cggtcagcgt ccgctcgtcg taatccagtt gcagcgcccc ggcgccgcac
    42901 acgcacacct tgcccatctg gcgggccacc agcgccgcgt gcgagctcac gccgcccttg
    42961 gccgtcagaa tgccctcggc ggcaatcatc ccgcgcaaat cctcgggcgt ggtctcgttg
    43021 cgcaccagca ggactttctc cccccgcccc gccgcttcca ccgcgcgctc cgcgttcaaa
    43081 tacatcttgc cggacgccgc gcccgggccc gcgggcagcc cccgcgcgat caccttggcc
    43141 gccgcctccg ccttggcgtc gaaaatcggc gccaggagct gctccagttg ctccgacgga
    43201 ttccggcgaa tcgccgtctt ccagtcaatg agcttctcct tgaccaggtc catcgagatc
    43261 ttgagcgcgg ccagcgccgt gcgcttgccg ttgcgcgtct ggagcatgaa aaccttgccg
    43321 tcctggatcg tgaactcgaa gtcctgcaca tccttgaaat gcttctccag cgtcccccgg
    43381 atctggttca gctccttgaa cgcctgcggc agatggttgg ccagttgcgc caccggctcg
    43441 ggcgtgcgca cgcccgccac cacgtcctcg ccctgcgcgt tcaacaggaa ctcgccgtaa
    43501 aactccttca ccccgttggc cgggttgcgc gtgaacgcca cgcccgaacc cgaattgtcg
    43561 cccgtgttgc catagaccat cgcctgcacg ttgaccgccg ttccccactc cgacggaatg
    43621 ccgtacttcc ggcgatacac aatcgcgcgg tcgttcatcc acgacccaaa caccgcgccc
    43681 gccgcgcccc aaagctgctt ccacggatcc tgcggaaact ccttgcccgt ccgctccttg
    43741 atcagcgcct tgaagcgcgt caccagctcc cgctggtcgt cggctgtcag cttggaatcc
    43801 tccacgtccg cgtggtattt ctcgtgcttg aagccatgaa tcaccacctc gaacggctcg
    43861 tgatcctcat tcgcgcgctt ctgcaccccc atcaccacgt cgccatacat ctgcacgaac
    43921 cggcgataac aatcccacgc aaaccgatcg ttcttcgtcg ccgccgccag cgcctgcacg
    43981 gtctcgtcat tcagccccag gttcaaaatc gtatccatca tccccggcat cgaatcccgc
    44041 gccccggacc gcaccgagac cagcagcggc atcgctttct tatccccgaa cttcgtgccc
    44101 atgatcttct cgatgaacgc aaccccggcc ttgacggcgg gctcgagcga tttgggatac
    44161 gtcttcttgt gctggtagta gtaggtgcag acttccgtcg tgatcgtgaa gcccggcggc
    44221 accggcaagc caatgcggct catctctgcc aggtttgcgc ctttaccgcc caacagcggt
    44281 ttcatgctgc cgttgccgtc agccttcttg tttccaaaca ggtatacgta ttttgttgtt
    44341 ttcgccataa agtctctgct ctaatcagta ctaacgttca gttcaccagg tttcgccgga
    44401 agtcttcctc cgaaacaatg cgcggaaaat accaaaaccc gccctcctgt caagcccaac
    44461 ccccatcgga tttccgcgcc ccaacgccgc cggaaccggc ccccaaaacg ccgctttcct
    44521 cccggctttg ccagcccaca gcccggttca tcccccggcc aatcccgccg gcccggcgcg
    44581 acccgcccct gactcgaaga aaccggcctc cgcccggcgt cccgggacac aacgccgcca
    44641 acattgccgc aagccgccac gcaacccgca agcgtcccgc cctttttccc catatcggcg
    44701 cgtgaaagcg caccgcctgc gagggatatg gtgcgccgca cgaggacaca ccgcctgcca
    44761 agtggcgcgg agtgctgcgt gaaaacccgc gtccggcagg gaaaagcgaa atcccgcgtg
    44821 aaaacacgcg tccctcaagg aggcgttctc ctccccattt tcccgccagc cgcgtgcccg
    44881 cacgcggcgt tcggcccaac caagggtcgg attagcgtct cataaggcac ccgctcccgc
    44941 cgccagccgc cccagcctcc ccagccagcc tatggctccc aataggccgt gtacaagggg
    45001 tgtaccaagg atgctcaaag ggggttcat
//
LOCUS       DAOPQG010000011        43478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1126_length_43478_cov_14.795892, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000011 DAOPQG010000000
VERSION     DAOPQG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 43478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43478
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1126_length_43478_cov_14.795892"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKI07_02240"
     CDS             complement(<1..220)
                     /locus_tag="PKI07_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49900.1"
                     /translation="MKMLTSPRTGSLGAETYYQSPFGVCARRRTVPRDRPSERKAAAR
                     SYFGISSREWGLKLSEAQRERWNAAALHV"
     gene            874..2250
                     /locus_tag="PKI07_02245"
     CDS             874..2250
                     /locus_tag="PKI07_02245"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOC49901.1"
                     /translation="MSDRIQWLWRESKRWVARGLISEDQAGQIRGLYPAPKAALPWGT
                     IIFAGFGAVTAGLGIILLLAYNWQAIPRGGKLAIVFAGLMMLHGVGIWLFQRGERWRQ
                     AGEAVCLLGSMLFGSGIWLIAQIYNIQEHYPNGFLIWGLGALLLAWAMPSLVQGLLAV
                     TVLGIWGCSESWAFEAPVDGGLLLILAGVGLLAWRLRSPFLFFFVLAAFVITLCANVS
                     VLHGFLLLRVLLQFGTVFLAVALLSERHGWFAEAAGIWKFYGWWCFLLPVYLLSFPFF
                     AERDIAGDLSDEAGLRLRQLVYGWGAFILSMLAWGWVEWSLRTGSARRGDDTSDLSGA
                     GGARVRQAVFPFENWLLPLTALLSQVLSLARLSNYRWEVAAVYNLIFLALAAAWIGRG
                     CREGLLRPTLLGSLLLVALVAARYFDLFENLAVRGVVFLVVGGLLIGEGIAFRRARQR
                     LQPAKATP"
     gene            2247..3281
                     /locus_tag="PKI07_02250"
     CDS             2247..3281
                     /locus_tag="PKI07_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HOC49902.1"
                     /translation="MKTKWIIGAVALQVLALAYLGGEREWVLRTGRTIYLRTIPVDPR
                     DVMRGDYVRVAYEMSSVSRAQCRGRLADRNQAFWALPPDTRVYARLRVGEDEVAEFES
                     LSAERPAEGVFLRGRTERSWSQQLDVRYGLEALFVQQGQGRPLEPSWEPEAVQVPLEM
                     KVALSPDGIGVLNGYRRSALGIGLRLEKEADTGSSAERQLRTIGATVELKNVSSNAVA
                     IVDLPGGRSLDLMVDPWRSAENWRWVHEAETPMTPAPDHVVVLKPGDSHLIKVDFQDA
                     WWSVVKKEQGTASQARPMKLTELTREGLEFCLEYRSPERAACARLPRSELIWHGRLRT
                     RTVRLWSIND"
     gene            3314..5497
                     /locus_tag="PKI07_02255"
     CDS             3314..5497
                     /locus_tag="PKI07_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOC49903.1"
                     /translation="MRSKRLVEGAHAYFLRHWQRWLRVRWRVRFSEDAFNLLMAAGVG
                     VIGGLVNIFFYYATELVRVLFLRQPGELVEVAERMAPWQRVVTPTLGGLCAGLVLYWG
                     LRMVGPQRSSNLLEVIVAGDGRLPLRTGLVKFLSSLVTIGSGGSIGREGGITQLSATF
                     ASKWGQLMKWHPYRLRLLVGCGAASGIAAAYNAPISGAVFAALIVMGNFSMSLFAPLV
                     CASVVATMESRSFFGIEPWYSVPPYVLTSVTQLPWFVCLGLVVGVLGAVFMKLLNAAS
                     AGFRRLPVPIFLRLALGGLLVGLIAVRFPEVWGNGYVAANRILHGEYGAPTFTARDIA
                     DLESLAGRLKQPAREDGVSTYLGALLAPETVVLLGDYQGGENRRLARALTADLNRIVR
                     SGALYETQRFAGVRLSEETRRMLERDAHGVDLARLNCRLLLEAYPHEMASAHWREAAA
                     TGLLVLAALVLAKLVATLATVGSGAVGGVFTPTLFLGAGAGATFALALQQLGAGEDFP
                     VAAFAVVGMGSMLSATTRSPLLAMIMVFEISLDYSLMPALMLACVLSMLVARRLHPET
                     IYTEPLRHKGLRVPQEPSASEAVAERSVGDLMRAPVPPVRETTTLQEIAARFLTSANN
                     FLPVVDAKGQLVGLVALQDLKEYLGSQEELRGIIAYDFMRPPPACVTPNQRLADVLSV
                     VLASEQRNIPVVNTLKENRLVGALPRAQVLRLFSEAIAASSKAEA"
     gene            complement(5557..6441)
                     /locus_tag="PKI07_02260"
     CDS             complement(5557..6441)
                     /locus_tag="PKI07_02260"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOC49904.1"
                     /translation="MTGLLVPLLLACGVLWCHKAGGAANTLPPAAGSGSANAGSLALL
                     DPDAAFLLRYGLGRRVAELAGIVCGALGMLLVGWLDDRHELRPRAKFAGQLLVAALVA
                     ASGTRITLFVPNVLFSYGITILWILTVVNAFNFMDNMNGLCAGIGAIGAWHLAIIAAA
                     DNQYLVGLIAFLTFGALLGFLPYNFPRARAFLGDAGSHLVGFLLAVLAILPHFYTEQQ
                     PRRWAVLTPLLVLAIPLADLVWVVLLRWRSGLPFYQGDTNHLSHRLVRRGLSPSLAVI
                     GIWAAAAALPLLSYLCLW"
     gene            complement(6609..7769)
                     /locus_tag="PKI07_02265"
     CDS             complement(6609..7769)
                     /locus_tag="PKI07_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC49905.1"
                     /translation="MRILHVITRLIIGGAQENTVSSVLGLRAKPGMDVQLITGPTTGT
                     EGSLDSVLANNSLPLTIVPPLVRPLHPWKDWMAWRELVRLFRVQAPQIVHTHSSKAGV
                     LGRLAAARAGVPIVVHTIHGPSFGSFQGWLANSIFKAAERHAARVTTHFVVVAEAMKQ
                     QYLAAGIGHPDQYTTILSGFALAPFLAAANDLQLRARLGLAPDDIVIGKIARLFKLKG
                     HDDLLTVAPELVRHCPQIRFLFVGDGPWRGRFEKRVRALGLEKHVVFRGLVPPDAVPA
                     LVGIMDLVAHLSLREGLPRALAQALAGARPVVAYDSDGAREVCMENETGFLLQPGDLA
                     GLRKRLLQLAQDRALRERLGRHGQQFVRERFGVERMVDRLYQLYRRLSGQPA"
     gene            complement(7779..10721)
                     /locus_tag="PKI07_02270"
     CDS             complement(7779..10721)
                     /locus_tag="PKI07_02270"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC49906.1"
                     /translation="MTSEPEVNPEKSFIRTFLPWLVAAGALALYLATLNHWISLGSLP
                     QVARLCGWTWQPELHGPLFWLVTYPFRWLPSHAIPLALNFFAAVCAALTLALLARSVV
                     LLPHDRTHEQRQKQKDKSPYLSIPAAWLPPVLAVLVCGLQLTFWEHATTASSQPLPPA
                     MPWGTGCEMLDLLVFAYVIRCLLEFRLDDRDSWLMRAAFAFGLGMTGNWAMIGFLPVF
                     LMALVWLKGLGFFNMRFLGRMSLCGLAGLLLYLLLPLLQVVGDSGAVGFWPALKANLG
                     AQRSILMLYYKYGRQVVALLALTSLVPLFIISIRWASYFGDSSRLGVWLATFMFHVVH
                     GLFLLACIWVAFDPPFSPRNRGFGAPFLTFYYLGALCIGYCSGYFLLLFGGSADRPRR
                     QSSHLRLLNNAITVFIWLLLVLTPLALICRNWTQIRTTNGPALKRYAEALALGLPAQH
                     SFMLTDDPRRAYLLQAYAARKGQSNRHMFLETAALQWPEYHRFLHRKYPQAWESNPPR
                     EVAQLAEPLMLAQLVSRLAQTNSLYYLHPSFGYYFEFFHLEPHGLIYELKPYSTNYLF
                     APPVDAKLVAENESFWAKINAEAIPPLLTSVAQTDPLPKPGLLEKLAKRAHLVQELNQ
                     DATTVAKFYSRALNYWGVEIQKTGRLTNAAAHFERALELNPDNIVAQINLECNRNQRV
                     GRSASVQVSKSIEDEFGKYRNWDQVIGENGPFDEPNFCFEQGRVFVRNSQYRQAAAQF
                     DRVKSLAPSNLDARIWLGQLYVVSLMPAEALRIIDEIHAQPDLAAAAVTNRTELLFVE
                     TSAHLGKDDPAGADAVVQASLRQYPNDMDLLATATQVYLKYGRFSNALSIIEQELILA
                     PTNVTALINQGYACIQVGAFEKAIPALTQVLTIETNNHSALLNRAIAYLRANKLAESK
                     DDYERLQKVFPTASQISYGLGEIAWRKRDTNAAIRNYELYLANAPTNTVEAKSVIARL
                     MELKPGAR"
     gene            complement(10863..12011)
                     /locus_tag="PKI07_02275"
     CDS             complement(10863..12011)
                     /locus_tag="PKI07_02275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOC49907.1"
                     /translation="MNLALGNKLSLRISDLAFGGEGVARWEDFVVFVPFVAVGELVEA
                     EITELKKRYARARLLRVLEPSPERVEPGCRYFGACGGCQYQHLAYSAQLRLKHKQIGD
                     LFERLGHFDRRLVAPVIPCPQPYGYRNRIMVRSQWDKFKQGLNIGFIRADSRLVVDVE
                     ECRLAEPALNEQLKYVRAHPPPKGGLKVVLRSSPPGWEVPPDSFFQNNSFLLPKLVEV
                     ARDALRQAQTKHLLDIYCGVGFFSVELAPLAESFVGVELDHMAIQAARRNAAARARTN
                     GEFIAGTAEEQLPAILSRFPAPATTVLLDPPRKGCQPETLQMLRRVQPAQIIYISCHP
                     ATMARDLNILCAQGAFELAQVRPLDMFPHTQHVECVGDLRCKAATGSP"
     gene            complement(12031..12243)
                     /locus_tag="PKI07_02280"
     CDS             complement(12031..12243)
                     /locus_tag="PKI07_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49908.1"
                     /translation="MTYEETAIMEFLRGTPDCFVARKEIARKALKRTVFEENPQWADA
                     PLVSLTNRRLIEQNENGHYRILKSER"
     gene            complement(12240..14747)
                     /locus_tag="PKI07_02285"
     CDS             complement(12240..14747)
                     /locus_tag="PKI07_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOC49909.1"
                     /translation="MSEQEAMSNFTPRAQQVLALARKEADRFNHNVIGTEHLLLGLIK
                     LGQGVAVNVLQKLGLDLDTVRLEVEKQVGTGPDQKMMGSIPYTPRVKKVLALAQKEAK
                     ALNHTYVGTEHILLGLLREGDGVAARVLKNLDVDIELTRKEVLKELDPNFAAADEPIP
                     GEAIPGKAAAEKKGEVKTPALKAFGRDLTEIARKGEMDPVIGRKSEIERVIQILCRRT
                     KNNPVLLGEAGVGKTAIVEGLAQEIAAGNVPELLRDRRLITLDLALMVAGTKYRGQFE
                     ERIKAVMDEIRRSRNIILFIDELHTIVGAGSAEGTMDASNIIKPALSRGELQCIGATT
                     LNEYRKYIEKDAALERRFQAVKVEAPSVAEAVLILKGLRPKYEEHHKAEFTDKAIEAS
                     VKLSDRYITDRFLPDKAIDVMDEAGARARIGTMTRPPEVKSLEAEIEEIKARKEKAIK
                     DQDFEGAASMRDKEKQAKEKLEALLNEWRTTREEKRVKVDEEDILQVVSKWTGIPLQR
                     MEQGEMQRLLQVEAEMDKVVIGQREAVSALCKALRRSRADLKDPRRPIGTFALLGPTG
                     VGKTLLAKTLAEQLFGDTKALIQLDMSEYMEKFNVSRLIGSPPGYVGYEEGGQLTEQV
                     RRKPYSVVLFDEIEKAHPDVWNMLLQILEEGKLTDNVGRVVNFRNTIILMTSNVGSDT
                     IRKQSTLGFSPITDESSYEKMREKILDEAKKTFRPEFLNRLDDIIVFRALTKPDLVQI
                     LDLEITKVMQRLKAKNIMLQLDDKAREFLVSKGYDPLYGARPMRRAVERSLEDPLAEE
                     ILRGAFQEGEPIIVTAENDHLAFSQKAAPEGALSSGA"
     gene            complement(14762..15874)
                     /locus_tag="PKI07_02290"
     CDS             complement(14762..15874)
                     /locus_tag="PKI07_02290"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOC49910.1"
                     /translation="MNLDAFLIPPAETARRRGPHDRIVMSSRVRLARNIRKAAFPGWA
                     KKAERLRVLELIQPAIAALPEMADAFFGTMDRLSALDKQILVERHLISREHAARNAGS
                     GLVLNRPETLSFMINEEDHVRMQALSPGLQLHQAWAIINGADSALEKKLDYAFSPELG
                     YLTACPTNLGTGIRVSAMLHLPGLVLAEQINPIIQSVSKLGLTVRGLYGEGTEASGNI
                     FQVSNQMTLGESEAAIIQRLEKVLNQLIEHEENARASLLERKPKLVYNHVGRAYGILA
                     NAHSISSKETMNLLSLMRLGSDAGLFPELDRALVDELFILTQPAHLQKQHSEKLSTEE
                     RDLLRADMLRERLRLISRPVIPPPAAAEGGLDKPAD"
     gene            complement(15871..16389)
                     /locus_tag="PKI07_02295"
     CDS             complement(15871..16389)
                     /locus_tag="PKI07_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOC49911.1"
                     /translation="MLCSICQKKEATVFLTNIAGDKMQKVNLCEECAKAKGMDDPTGF
                     SFADKLLGLGATQEIEQAGGEKGLRCPVCGFTQSDFRKAGRLGCPECYETFAEPLKAV
                     LQTMHKGTRHVGKVPETQRQGHNLAAELKQLQQKLAKAIELENFEEAAVLRDEIKQVT
                     ARLSQHAAERPV"
     gene            complement(16397..17269)
                     /gene="ilvE"
                     /locus_tag="PKI07_02300"
     CDS             complement(16397..17269)
                     /gene="ilvE"
                     /locus_tag="PKI07_02300"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOC49912.1"
                     /translation="MKIYIDGKFYNERDAKISVFDHGLLYGDGLFEGIRAYQGRIFKL
                     KEHIDRLHCSARSILLRLPLTSRELTAAIIATCRQNRLRDAYIRLVVTRGIGTLGLNP
                     NRCKRPSVIIIAGKITLYPAAFYERGMEIITVPTTRNLHSALNPAIKSLNYLNNVLAK
                     IEANNGGCEEAIMLNAEGFVAECTGDNLFIVKDGRLLTPPLSAGALYGITRQVVMELA
                     AETGRKAIESNLTRYDLFNADECFLTGTAAELVPVVKIDGRVIGNGKPGRVTRGLMAQ
                     YRALTKVSGEPIYD"
     gene            17543..18625
                     /gene="dusB"
                     /locus_tag="PKI07_02305"
     CDS             17543..18625
                     /gene="dusB"
                     /locus_tag="PKI07_02305"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOC49913.1"
                     /translation="MASNLFLSPLAGYTNLPFRLTVRELGGLGLATTDLVNARSLLEK
                     RAKALKLIETSPADRPLAVQLFGSVPEEMRDAAACLESLGVASVDVNMGCPARKVCRV
                     GGGSTMMTELARTARLVKGMVDAVRIPVTAKMRLGWDEENLTAPELARALEEAGVAGV
                     FVHGRTRAQGFRGQVNLAGIRAVAQAVKKIPVIGNGDVTTPEAAQTMLNQTGCAGVGI
                     GRGAFYNPWIFQHTRHYLQTGQLLPEPSFEERVRVMCRHLDRMIEVFGEAHGCRMFRK
                     IAPWHARRFGPAKEFTHRVVRVSSRAEFLEILERYRIWRRQFLDSDGELLPRFRPPPL
                     VASFLRSPDPAQDGQFPTPKGPVEMW"
     gene            19134..19277
                     /locus_tag="PKI07_02310"
     CDS             19134..19277
                     /locus_tag="PKI07_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49914.1"
                     /translation="MPKSLILPAKGFGFGSYALEFVKQEVLQSYGLDAQTNPGFNETS
                     KGD"
     gene            19782..21257
                     /locus_tag="PKI07_02315"
     CDS             19782..21257
                     /locus_tag="PKI07_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC49915.1"
                     /translation="MAKVLLVDDDLTTLQVVSELLRQEGHEVFPFSNGNAAIAGLTAH
                     APDLVITDLYFDKTRAQGLEVVEKARALSPPAEVIVITGFGTIETALRATKLGAYDYL
                     EKPFKVEELKLCVQRTLSYSAARNETVHLRRQLKKKYGFDQIVGSAPKMVEVFKMIER
                     VANTDSTILVLGESGTGKELVARALHYNSRRQFAPFVPVNCSALPENLLESELFGHCR
                     GAFTGAIGDKKGLFEEADGGTIFLDEVGSMSAMLQSRLLRVLQEREVRRVGENRPIYV
                     NVRVVAASNEPLEKRIKEGSFREDLYYRLNVIAIPLPSLRERREDIPLLTAHFLKGKM
                     APAASQPLRITRQAMEILRRYDWPGNVRELENALERAAVLCEGGLIQAADLPPHLLAA
                     VQDADLMPETQTAPPLPAVPDSAVYPLHPPGAPETASAAAAPGADAPIIPMKAYLQQQ
                     ELAYLNRAIQQCGGNKEQAALQLGISVATLYRRLNAEEKEP"
     gene            21375..22337
                     /locus_tag="PKI07_02320"
     CDS             21375..22337
                     /locus_tag="PKI07_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOC49916.1"
                     /translation="MPLTHTKELFARALKGKYALGAFNVNNMELLQAIVEACEEEKAP
                     VMLQISKGAREYANPVYLKKLIEAAISLSNIPVAVHLDHGDSFELCKDCIDEGFTSVM
                     IDASHEPFEKNVAICRKVVEYAHAHDAVVEGELGCLIGAQFDDGEEGGSFSKEGRYTN
                     PEQAVEFVKLSGVDSLAVAIGNSHGAYKFKGEQHLDLERLKAIKQAFQDAGLGDYPLV
                     LHGASSVPKSLVDEINKYGGKLTDAAGVPEGDIEVARRLGCAKVNIDTDLRLAMTAAI
                     RKVFAENPREFDPRKYLGPARAKVKELVQHKVRNVLCCAGHAFD"
     gene            22374..23081
                     /locus_tag="PKI07_02325"
     CDS             22374..23081
                     /locus_tag="PKI07_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOC49917.1"
                     /translation="MNYEAFYKISYGLYVVSSAHGGQRSGYVGNTVFQVTSQPARFAI
                     ACNKDNFTCELIRQSQGFAFSILQQDTRAELIGLFGYKSGRAVDKFASVRHRTGQTGV
                     PILLEDALAWFECKVVQTLDVGTHLLFIGEVVAGELISDAQPPLTYAYYREVKKGKAP
                     KNAPTYIPPASTAAAPPAAAAPAGKYVCTVCHFVYDPEQGDPHAGIPAGTRFEDLPEG
                     WVCPVCGAPKSDFVPQT"
     gene            23103..23348
                     /locus_tag="PKI07_02330"
     CDS             23103..23348
                     /locus_tag="PKI07_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOC49918.1"
                     /translation="MTGMKTPEIVQKSPAVQKVEPGTYYWCACGKSKTQPFCDGSHRG
                     TEFAPVRVEISEAKTVAWCLCKHTKTPPFCDGTHQQL"
     gene            23494..25578
                     /locus_tag="PKI07_02335"
     CDS             23494..25578
                     /locus_tag="PKI07_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOC49919.1"
                     /translation="MRAQIIPAGGLRCLVRVVGVAFGIAAGVWLAGASCGAAPAKTTA
                     KAVPVPPTASVNPFYPGNREPLLPSPLIKLPIGSIQPRGWLRHQLELEAQGMTGRLPE
                     ISKWCKFEGNAWADPQGRGHSSWEELPYWLKGYGDLGYVLGDPVITQNARKWVEAVLA
                     SQQADGWFGPIANKTGLKGKPDLWPHMVMCNVLQSFYEYSGDARVLPFLTKYFRWLNT
                     LPDECFGAGYWPRIRFGDNVETLQWLYNRTGEPWLLELARRIHENMEDWTRGVHNWHN
                     VNLAQGFRAPGVYYQQAKEPRFLAAVERNYQTIMERYGQFPGGGFAGDENCRPGYTDP
                     RQGFETCGIVEFMHSFELLTKISGNPLWADRCEEIAFNSLPASLTPDWKGLHYLTCPN
                     QIQLDKENKAPAIQNKGTMFSYSPFERYRCCQHNVSHGWPYYAEELWLATADRGLCAS
                     LYAACQVTAQVGEGTRVTIAEATDYPFGDTVTLRLSMPQAVSFPLYLRIPRWCARAAI
                     SVNGRPVRVAAPPLHYVVLERAWQDGDTVSLRLPMQVAVRRWEQNQNAASVDYGPLTF
                     SLKIGERWSRCGGTDAWPELEVFPTTPWNYGLVLDPKCPARSFKVVRGPRSAGLAGQP
                     FEPAAAPIELRVQARRIPDWKQDALGLVGKLPLSPVRSREPVESITLIPMGAARLRIT
                     SFPVIASGKIAE"
     gene            25609..27891
                     /locus_tag="PKI07_02340"
     CDS             25609..27891
                     /locus_tag="PKI07_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOC49920.1"
                     /translation="MKQTRLYQRNPLAAGICLALLFLTPAPARDAWQPAAGPLKTRWA
                     KEVSPHKVHPEYPRPQMVRKDWLNLNGLWDLAVTGKMAARANFQERILVPFPIESALS
                     GIGRPVSENDRLWYRRTFTLPPQWQGRRILLHFGAVDFDTTVLVNGRKVGHHRGGYDA
                     FSFDITDDVRPHGENELIVSVWDPTDAGTQPIGKQVRQPRGIWYTATSGIWQTVWLEP
                     VHAVHISGLKITPDVDAGAVTVEAITPPLLATCTLEMTIRDRGKVVHAASLSGGRQIT
                     MPVKNARLWSPEDPHLYSLDVRLKIGRRTLDRVESYFGMRKISLGKDARGFTRLLLNN
                     KPYFQFGLLDQGFWPDGLYTAPTDAALRYDIEMTKKLGFNLARKHVKIEPERWYYWCD
                     KLGLLVWQDMPSGEKHIGPKDPDITRSPESAAQFEQELTAMVRGRYNHPCIVMWVPYN
                     EGWGQWDTARIVELVRKLDATRPVNNASGWTDRGVGDVNDLHKYPGPGSPEPEANRAV
                     VLGEFGGLGLPIRGHTWQSEKNWGYRSFTDSETLTDAYIDLVEKLFPLIDEKGLSAAV
                     YTQTTDVEIEVNGLLTYDRERVKMDARKITAANRGQFLPRPRVTEVVPTALTERPAWR
                     FTLEKPAADWHQPGFDAGAWKEGQAGFGTKGTPGAVVRTEWNTSDIWLRRAFDLPAGK
                     SDDLRLLIHHDEDAQVYFNGVLAASVPGYTSDYGQVRISPEARAALKPTGNIMAIHCR
                     QTGGGQYIDAGIVKIELPKN"
     gene            27891..29180
                     /locus_tag="PKI07_02345"
     CDS             27891..29180
                     /locus_tag="PKI07_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOC49921.1"
                     /translation="MRRRALLLLLCAANLATAAARPDHCSVRDFGAKGDGHTDDTAAF
                     QKALDKMGRAGGGVVYAPRGNYFFAGHLTVPVAVTLKGVWESVPAHVGLRNPGWNKPT
                     DDGTTFLVTENRGREDGPPFITLNADATLKGVVIYYPGQNPEVEPEPYPYAIALRGKN
                     PAVLAVELLNPYNGIDATRNERHLIRDVHGQPLRRGILVDDIYDIGRIENVHFNPWWS
                     TKPRLRQWLQQHGEAFIFGRSDWQYTLNTFCFGYHIGYKFIRTATGLCNGNFLGIGAD
                     DCHTALFVENCAPMGLLISNGEFVSFHGPDPTMVRVGTNNVGSVRFVNCAFWGPCCQI
                     ATVAGKGTVGFSDCTFVQWDRENEGRPALQIMSGTALVRGCEFQADKPQIELGKEVRR
                     AVITDNLITGMLRITNHSPATVIIRHNAGDSPGQPQE"
     gene            29177..31306
                     /locus_tag="PKI07_02350"
     CDS             29177..31306
                     /locus_tag="PKI07_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOC49922.1"
                     /translation="MMTARRFALTAGLAASLLGCARPATKPAAPAGLPPPAPGVFFVA
                     TNGHDHWSGQRPRPNRQRTDGPFATLPRAVQAARAFRQQSASPAARPASIFVGGGTHF
                     LAQPLALTPADSGLTLAAYPDTQPVLSGGRPISGWREVTIAGRRLWCADLPAELRGQW
                     SFQQLWVNGQRATRARHPNHGYLAVAGLPDKTKDWTQGHSRFRFHPGDLPAGETITQA
                     EVVVMTRWVESRLPIAGVDEKRRLATFGKRSVFALASGDLYYAEGAFEFLDDPGEWHL
                     DRTRGRLFYLPRPGETLARAQAIAPMLAQVLRFEGRPEAGQFIERISLRGLTFSHTEW
                     HFPSELPSAAEQPKVWPPPATEVGGFAQAAVGVSGAVYGEGLRHCLFADCRFAALGNY
                     GLELSRGCQSNHIARCEFTDLGAGGLKLGETRIRDQAAEQTRANEISHCHLHDGGKLF
                     ASAVGIWLGQSPDNRILHNHIHDFYYTGISIGWTWGYGPALAGNNLVALNHVHHLGQR
                     SNGDGPILSDMGGIYTLGKQPGTTIRSNLWHDISGFRYGGWGIYFDEGSSGILAEDNL
                     VYRTTHGGFHQHYGETNLVRNNLFAFARNHQLQRTRPEPHRSFSFVTNIVYFDSGNLL
                     GGDWSNDQYLMDANLYFDARPDAQPDQLRLGPCTWQQWQERGHDRHSLVANPLCVAPD
                     KDDFRLQPASPAFRLGFRPTDLSNVGP"
     gene            31927..34689
                     /locus_tag="PKI07_02355"
     CDS             31927..34689
                     /locus_tag="PKI07_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOC49923.1"
                     /translation="MNYFGIVFALLLGSMVSSPATQEGRDLPETPHPEPVRGPAPDNM
                     PVKGALPGQSGVPKALLGPIAPAGAQPTGALSGRIVFTSGGHGLAWDGASWTTGRGVN
                     WEMVEDYGNVDQMSMFAYYCFNAGATVVAMRPIGNQTNEVVLDNVDPEVTFQGAWADS
                     VFTNYYGNAGDVPYRFTSVAATETATATYVPNIPVAGFYPVYTWVWHSTNRTSQLYRV
                     RHTGGESQVRVPHYLVGGGWVYLGTYYFAAGSDAARGAVVISNLAPSPGVGSAVIADA
                     IRFGNGMGSIARGGGVSGHPREHECARYWIQSSLGRGSPTWIYDDPSLIDSDDNVSAP
                     IRMAREMNEEAAGNFYQRIYIGFHSNASGLGTNSSARGDIGLYNNDNLFPGTATSNQF
                     RLAEIIATNVNNALKRITVPPFEVPWLNNRSSLTYARTDFAFGEIRGDRLGYEMDATI
                     IEVAFHDNVSDARLMRDPKVRNWIARASYQAVVHYMHQFDAVPLNFLPEPPRHVRAVA
                     TANGIQVRWDAPVAEFSTGTPAGFVVYRSTDGYGFGEPVEVAGGGASMIEFTNVLADT
                     DYYFRVAAVNAGGESFPSETVGCRRASNPAHSRVLFVNGFDRFDRTTNLRQNPTGAYA
                     PPGNSGAMERVIPRSNNSFDYVVPHGKAISAAGLPFDSCSRPSVTNHQVALTNYDIVI
                     WACGQSLTNTFRAPERNVVTAFQNGGGHLFLTGSEVAWDLDRAAGPSAADRAFLNNQL
                     HADLGPDANNNSSNYTFAAVAGTIFEGNSGGAFDDGNQGIYWVKTPDVLVPTGSGATA
                     ALVYGGGGGKVAGIQYAGASGGRVVYWGFPFETVTDEAIRADYMADVLRFFARPPRFE
                     RVSFGAGPSVRLWLNGDPGEYTLQTSPDLINWAFWTNVQMGLQPIEISDDSFTSAPRR
                     FYRALR"
     gene            34933..36990
                     /locus_tag="PKI07_02360"
     CDS             34933..36990
                     /locus_tag="PKI07_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOC49924.1"
                     /translation="MLGRNLPPWRGGLALALSLLMMIASGDAGARSRPEVRELNPQIE
                     AAIAKVKPALVRIRVVETDYSEGRETKSQAVGSGVIITRDGYLVTNHHVAGHAVRIFC
                     TLWNREEIEAELVGTDALTDIAVLRLTPARPVEFKTASFGDSSKVQAGDYVLAMGSPM
                     ALSQSVTLGIISNPEMIMPRSGRGERAFRLDGEDVGSLVRWLGHDAAIYGGNSGGPLV
                     NLRGEIIGINEISYGLGGAIPGNLARSVAEQIMAKGRVVRSWLGLDVQPRFKRSTEPH
                     GVLISGVQAGSPADQAGFKPGDLLLRLGGEATDVRFDEQMPDFMLLATSLPLGQETRA
                     VVKRNGKELTLRPVPIERGEVYPPQQELKAWGLTVRDLSRLLAQEMKRADPVGALVTS
                     VRPGGPAGTAKPALQAKDVIVAVNDQPVRSTRDLVALTRKLTEGRTEPLPVMATFERR
                     AARLLAMVRIGLDELKDPGLEVTKAWLPVETQVISREIARQLGQPALKGFYITRVYPG
                     TTAEQAGLKAGDFITAVDGEKLSASDSEHEDELSALIRQYDVGQTVTLTIRRGQAEHK
                     LPVSLARSPRLQREMRKYRNEDFEFTARDVSFFDVVEEQWSRDQLGVLVEEVKPGSWA
                     ELGSLFKGDLILEVAGQPVDKVDALRQELERAAARRDQVVRFKVLRGIHTTYLEIEPN
                     WKN"
     gene            37011..37847
                     /locus_tag="PKI07_02365"
     CDS             37011..37847
                     /locus_tag="PKI07_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOC49925.1"
                     /translation="MRYPYSILRAALALALAGAACSLRADELGDKGRDIFRKYQQTVV
                     TVQVVQKITYSGAAKSGENRQELTGTVVDPSGLTVLALSACDPSEMYQRMLAQQGSQN
                     KLEIEVADMQILLADGTELAAEIVLRDKDLDLAFVRPKSKPATPMTAVDLARSSTVQI
                     LDQVITLNRLNSAAGRAYAASVERINAVIQKPRTFYVPDSTMTSTTLGSPAFALDGNI
                     VGVVVMRAVASGGGASRSVRENLSAIILPAEDILKAAKQAPEAKGEPGKKEEAKDAQP
                     GK"
     gene            38010..40160
                     /gene="recQ"
                     /locus_tag="PKI07_02370"
     CDS             38010..40160
                     /gene="recQ"
                     /locus_tag="PKI07_02370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOC49926.1"
                     /translation="MESSVPQLLPLLKQYFGFRSFRPLQEEIIRDVLAGQDVFALLPT
                     GGGKSLCFQLPAMARPGLAVVVSPLIALMKDQVDALQASGVPATFLNSSLAAGESRAR
                     LRGLHNGEFRLLYVAPERLMLPGCLADLQRWGVNLLAIDEAHCISEWGHDFRPEYRQL
                     AELRQLFPTVPMMALTATATARVRHDIVTLLRLREPRCYVASFNRPNLTYRVFAKHKP
                     YDQLRKFLRERPGQSGIVYCQARKTAESVASRLEADGLKAKPYHAGLTAKERTAHQEL
                     FLQDEVRVICATIAFGMGINKPNVRFVVHYDLPKNIEGYYQETGRAGRDGLPSECVLL
                     FSAGDVAKLVRFIDEKSDPGERNIAHEQLRQMVHYAESSGCRRSELLGYFGEPAAEAN
                     CGGCDNCLSPRATYDGTLSAQKFLSCLYRIRQRSGFEFGMNQIVEVLTGAETESIRRW
                     GHEQLSTYGIGQEHSRAEWKAIGRELIRQGLVRQVSRDHFSVLGLTEDGLAVLKERKP
                     VRLTRPVSAPEPKAPRAGEIACDELLFERLVRLRKQLADERALPPHILFSDVTLRQVA
                     RDYPASERALARISGMSARKLQEFGRVLLAVVAGHLETQPRQIFAETSFEDDVPREAR
                     STRRASSDAAPYDKELFERLRMVRRRMAERRGLPAYMILHDSALREMARRYPRSIEEL
                     TEIARVGSRRASQFGEEFLKVIAEHECASPQASD"
     gene            complement(40292..42829)
                     /locus_tag="PKI07_02375"
     CDS             complement(40292..42829)
                     /locus_tag="PKI07_02375"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOC49927.1"
                     /translation="MKRNYYCLAIVLLFMALLASTGVYASEADIQIPPLDTVKFAGLQ
                     GLSGTALMYVGLVICVIGAVFGLVQYSQTKALPVHDSMRDVSHMIWETCKTYLFTQGK
                     FLAILWALIAACMIYYFGVLSKMNLVNVIVILLASILGILGSYGVAWFGIRINTVANS
                     RAAFSALRGNAFATLSIPLRSGMSVGLLLVAVELFFMICILVFLPRDLVGPCFIGFAI
                     GESLGASVLRICGGIFTKIADIGSDLMKIVFKLPEDDPKNPGVIADCTGDNAGDSVGP
                     TADGFETYGVTGVALIAFLALALAETPGIVATLIIWIFAMRALMIVTSLASYLGNQAF
                     SKAKYGHQKDFDFEAPLTHLVWITSAVSIAITFIASKLLLGDFTDAAGRALPDLWWVL
                     SIIISCGTVAGALIPEFTKIFVSTGSRHVREVTNCSKHGGASLNILSGFVAGNFSAFW
                     MGLCILLLMFVSYYFSQNEALLAIMPKQFAFAAPIFAFGLVAFGFLGMGPVTIAVDSY
                     GPVTDNAQSVYELSQIESRKGIKEEIKKGFGFEPDFENAKYQLEKGDGAGNTFKATAK
                     PVLIGTAVVGATTMVFGIIILLQHMFEANPTSGVFKPVVEALSIVQPEIILGLIMGGA
                     VIYWFTGASCQAVVTGAYRAVVYIKEHMKLDASTASEKDSKEVVRICTVYAQKGMWNI
                     FIVIFCLALALPFFNPYFFIGYLIAIAFFGLFQAIFMANAGGAWDNAKKIVEVDLRQK
                     GTDLHAATVVGDTVGDPFKDTSSVAMNPVIKFTTLFGLLAVEIAVTMPNQNLKTGIGA
                     FFFLVALIFVYRSFYLMRIPEEDRAGTNGNLVLPHKVHREEVAGGRK"
BASE COUNT         8207 a        13077 c        13914 g         8280 t
ORIGIN      
        1 ggacgtgcaa tgctgcggcg ttccagcgct cgcgctgcgc ttcgctgagc ttgagacccc
       61 actcccgtga ggagatcccg aaatacgacc gtgcggccgc cttgcgctcc gagggtcggt
      121 cccggggaac ggtgcgccgc cgggcgcaca cgccgaaggg ggattggtag taggtttccg
      181 cgcccagact gcccgtgcgc ggagaggtga gcattttcat agttaattac cttttcgtta
      241 ttgccccgga gagcgactcg gcttcctgcc attccttgga ctggaagtcc cagccaccac
      301 caccccgagg gttttgggga aggctggcgg gcgggaggtc ttcgcgcaag ggcaattttt
      361 tggggttggc gatggcgggc agaatccttt gttatcggcg cctgagtttc tgcaaaatag
      421 ggagaaaagg ttttcctaaa cccaacgccg cttttcgggt tgaggggact ggtatgacag
      481 agtctgccaa ttcaaagcgg ctgggcgacc ttgggagcaa cgctgcccgc ggatgcgaaa
      541 gacacggtgt gatgcgaacc aatggccacc gccccgcggg aaagcttgcc ggccttgaaa
      601 ggcaagccta tcaccaccaa ccctttgcac gaccaacgca aactggcccg gaggatcagg
      661 caaaagggcg gcgacgatct ccttccgagc gaagttaacc agcccctctg tttccaaccg
      721 ccctgatctt gggcgcacgg ccagacagct cttccgaagc gcttaactcc gccccatccg
      781 cggtctcaat cttcccagct caatatcaac aaggccaaag gccctgttga ccctgggaac
      841 gggctcctat agtctttcca cgatgaggcc tcaatgagtg accgaatcca gtggttgtgg
      901 cgggagtcga agcgttgggt ggcccggggc ttgatttcag aggatcaggc agggcaaatc
      961 cgcgggcttt acccggctcc caaagccgcg ttgccgtggg gtacgattat cttcgccggc
     1021 ttcggcgcgg ttaccgctgg cctggggatt attttactgt tagcctataa ctggcaggcc
     1081 attccacgag gcggcaaact ggcgattgta tttgccgggc tgatgatgct gcacggggtg
     1141 ggcatctggc tgttccaacg gggagaacgg tggcggcagg caggcgaagc ggtgtgtttg
     1201 ctgggcagca tgcttttcgg ttccggtatc tggcttatcg cccagattta taatatccag
     1261 gagcactatc cgaatggctt cctgatctgg gggttggggg cgctgctgct ggcctgggcc
     1321 atgccttcgc tggtccaggg acttctggct gtcacggttc tcggtatctg gggctgctcc
     1381 gaaagctggg catttgaagc cccggtggat ggagggctcc tgctgatcct ggcgggggtg
     1441 gggttgctgg cgtggcgatt acgctcaccg ttcctgttct tttttgttct ggcggccttc
     1501 gtcattacct tgtgtgctaa tgtgagcgtg ttgcacgggt ttctgctgct gcgcgtgctc
     1561 ttgcagttcg gcacggtatt tctggcggtg gcgctgctgt cggagcgcca tggctggttt
     1621 gcggaggcgg caggcatttg gaagttttac ggctggtggt gttttctgtt gccggtttat
     1681 ctgctgtcat ttcccttttt tgcggagcgc gatatcgccg gggatttgtc tgacgaggcg
     1741 ggcttaaggt taaggcagtt agtttatggt tggggggcct ttattttaag catgttggct
     1801 tggggatggg tggagtggtc gctgcgaacc gggtcagccc ggcgggggga tgacacctcg
     1861 gacctttccg gagcgggagg cgcccgcgtc cgccaggcgg tcttcccctt tgaaaactgg
     1921 cttttgccgt tgaccgcgct cttgagccaa gtattgagcc tggcgcgatt atccaattac
     1981 cggtgggaag tcgcggcggt gtataacctg atatttctcg ctctggcggc ggcctggata
     2041 gggcgtggct gccgcgaggg actgctgcgc ccgaccttgt tgggttccct tttgttggtg
     2101 gcattggttg cggcccgcta cttcgacctg tttgagaatt tggcggtgcg aggggtcgtt
     2161 tttctggtgg tgggaggttt gctgattggg gaaggaatcg cgtttcgccg cgcgcggcaa
     2221 cggctgcaac cagcaaaagc aacgccatga aaacgaaatg gatcatcggc gcggtggcct
     2281 tgcaggttct ggcgcttgcg tacctgggcg gtgagcgcga atgggtattg cgcaccgggc
     2341 gcacgatcta cctgcgaacg attcccgtgg atccgcgtga tgttatgcgg ggcgactacg
     2401 tccgggtggc gtacgagatg tcgagcgtgt cgcgcgcgca gtgccgcggc cgcctggctg
     2461 accgcaacca ggcgttttgg gcgctgccgc cggacacgcg ggtctatgcg cgcctgcgcg
     2521 tcggggagga cgaagtggcg gagtttgaga gtctttccgc ggagcggcca gccgaagggg
     2581 tgtttttgcg agggcgaacc gagcgatcct ggagccagca actggatgtg cgctatggct
     2641 tggaagcgct atttgttcaa caagggcaag gccggccatt ggaaccgtcc tgggagcccg
     2701 aggccgtcca ggtaccattg gaaatgaagg tggcgcttag tccggacgga ataggggtgc
     2761 tcaatggcta ccggcggagc gcattgggca ttggccttcg cctggagaag gaggcggata
     2821 ccgggagcag tgcggagcgg cagttgcgga cgattggggc tacggtggag ttgaagaatg
     2881 tcagttcgaa tgcggtggcg atagtggatt tgccgggcgg gcgttctttg gatctgatgg
     2941 tggatccttg gcggagtgcg gagaactggc gctgggtgca tgaggccgaa acgccgatga
     3001 caccggcccc ggatcatgta gtggttttaa agcccggaga cagccatttg attaaggtgg
     3061 attttcagga cgcctggtgg tcggtggtca aaaaggagca agggacggcg agccaggctc
     3121 gccccatgaa gctgactgaa ttgacacggg aggggttgga gttttgcctg gagtatcggt
     3181 ctccggaacg cgcggcctgc gcgcggctgc ccaggtccga attgatctgg catgggcggt
     3241 tgcgcacgcg cacggtacgc ctctggagta ttaatgattg accggattgg agctgccccc
     3301 gcttgtgccg tggttgagat ctaaaaggct ggttgaaggg gcgcatgcgt acttcctgcg
     3361 acattggcag cgctggctgc gggttcgctg gcgggtgcgc ttcagcgagg atgcgttcaa
     3421 tctgttgatg gccgccgggg tgggagtgat cggcgggctg gtcaatatct ttttttatta
     3481 cgcgaccgag ttggtgcggg tgttgttttt gcggcagccg ggcgaattgg tcgaggtggc
     3541 ggaacgcatg gcgccctggc agcgggtggt cacgccgacc ctgggcggat tgtgcgcggg
     3601 gctggtgctt tactggggtt tgcgcatggt ggggccgcaa cgttccagca atctgctgga
     3661 ggttatcgtg gccggggatg ggcggctgcc gctgcgcacc ggcctggtca agttcctttc
     3721 ttccctggtg accattggtt ccggcgggtc gattggccgg gaggggggga ttacgcaact
     3781 gtcggcgacg tttgcctcca aatgggggca attgatgaag tggcatccgt atcggctgcg
     3841 gctgctggtg ggctgtggag ccgcctcggg gattgcggcg gcttacaacg ccccgatctc
     3901 cggggcggtg tttgcggcgc tgattgtgat ggggaacttc tcgatgagcc tgtttgcgcc
     3961 gctggtctgc gcgtcggtgg tggcaactat ggagtcgcgc agcttcttcg gcatcgagcc
     4021 ttggtacagt gtgccgccct acgtattgac gagcgtgacg cagttgccct ggtttgtgtg
     4081 cctggggctt gtggtgggcg ttttgggggc ggtgtttatg aagctgctga acgcggccag
     4141 tgccggtttc cggaggctgc cggtgccgat tttcctgcgc ttggcgctgg gcgggttgct
     4201 ggtggggctg atcgccgtgc gttttccgga agtgtggggt aatgggtatg tggcggccaa
     4261 ccggattttg catggcgagt atggcgcgcc gacgttcacg gccagagata ttgcggactt
     4321 ggaatcgctg gcgggcagat tgaagcagcc ggctcgcgag gatggcgttt ccacctacct
     4381 gggagcgctg ctcgccccgg agacggtggt gctgctgggc gattaccagg gcggtgaaaa
     4441 ccggcggctg gccagggcgt tgacggctga tttaaatcgc atcgttcgga gcggggcgct
     4501 ctacgagacg cagcgatttg ccggcgtgag attatcggag gaaacccggc gcatgctcga
     4561 acgcgatgcc cacggggtgg acttggcgcg gctcaactgc cggttgctgc tggaggcata
     4621 tccgcatgag atggcatccg cgcattggcg ggaggcggcg gccaccgggc tgctggtatt
     4681 ggcggcgctc gtgctggcca aactggtggc gaccctggcg acggtgggct cgggcgccgt
     4741 ggggggggta ttcacgccca ccctgtttct cggggcgggt gcgggggcga catttgcgct
     4801 ggcgctgcag caattagggg cgggggagga cttcccggtg gcggcctttg ccgtggtggg
     4861 gatggggagc atgttgtcgg ccaccacgcg ctcgccgtta ttggcgatga tcatggtgtt
     4921 tgagatttca ctggattact cgctcatgcc ggccttgatg ctggcctgcg tcctgtccat
     4981 gctcgtggcc cgccggctcc atccggaaac catttacacc gagccgctgc gccacaaggg
     5041 tttgcgcgtg ccgcaggaac ccagcgcctc ggaagccgtc gcggaacgca gcgtcgggga
     5101 tttgatgcgg gcgccggtgc cgccggtgcg ggaaacgacg acgctgcagg aaatcgccgc
     5161 gcggtttttg accagcgcca acaattttct gccggtcgtg gacgccaagg ggcaattggt
     5221 cggcctggtg gcgctgcagg atttaaagga atacctgggg tcacaggaag aattgcgcgg
     5281 gattattgct tacgatttta tgcggccgcc gccggcttgc gtgacgccca atcagcgcct
     5341 ggcggatgtt ttgtccgtgg tgctggcgag cgaacagcgc aacatcccgg tggtgaacac
     5401 gctgaaggag aatcgtttag tcggggcttt gccgcgggcc caggtattgc gcttgttttc
     5461 ggaggccatc gccgcaagca gcaaggccga agcctgacgc gcagaaaggg cgtgtgggcg
     5521 cgcaacgggc gaagctgcgc tttgcggtca tcccccctac cagaggcaaa ggtaactcaa
     5581 aaggggcaac gctgccgccg cggcccagat gccgattacc gctaaggatg gggacagccc
     5641 ccggcgaacc aggcggtggg acagatggtt ggtatcgccc tggtagaacg gcaggccgct
     5701 gcgccagcgc agcagcacca cccagaccag gtcagccagt ggaatggcga ggaccagcaa
     5761 gggggtcaga accgcccacc gccgcggctg ctgctccgtg taaaagtgcg gcagaatggc
     5821 cagcaccgcc agcaagaacc cgacgagatg gctgccggcg tcaccgagaa aggcccgggc
     5881 ccggggaaag ttgtagggca aaaagccgag cagggcgccg aaggtcagaa aggcaatcag
     5941 gcccaccaga tactggttgt ccgcggcggc aattattgcg aggtgccagg cgccgatggc
     6001 gccgattccc gcgcacaggc cgttcatgtt atccatgaag ttgaaggcat tgaccaccgt
     6061 gagaatccac aggatggtga tgccatagct gaagaggacg ttggggacga acagggtgat
     6121 gcgcgtgccg ctggccgcga ctagcgctgc cacgagcaat tgcccggcaa acttggcccg
     6181 cgggcgcaac tcatgccggt cgtccagcca tcccactagc agcatgccca gggccccgca
     6241 gacgatgccg gctaactccg caacccggcg gcccaggcca taccgcagta aaaaggccgc
     6301 gtccgggtcc agcagagcca ggctgcctgc gttggccgag ccggaaccgg cagccggggg
     6361 gagggtgttc gccgcaccgc cagccttgtg gcaccaaagc accccgcatg ccagcagcaa
     6421 agggacgagc agcccggtca taatggccaa cccgccggcg agcgggattg gttggtcatg
     6481 aatcttgcga tggccgggtt catccaccag cccgatacgc agacaccagc gtcgccaaag
     6541 cggcaatcca accgctacgg tgacaccggc acttacgcca gcccaaaggt aaacattaaa
     6601 gggaaatatc atgccggttg gccgctcagc cgccggtaaa gttgatacag gcggtccacc
     6661 atgcgctcta caccaaagcg ctcgcgcaca aactgctggc catgccggcc caggcgctcg
     6721 cgcagcgcgc ggtcttgcgc taattgaagc aggcgtttgc gcaagccggc caggtctccg
     6781 ggctgcaaca agaatccggt ttcattctcc atgcaaacct cccgggcccc gtcggaatcg
     6841 taagcgacga cggggcgggc tccggccagg gcctgcgcca gggcgcgagg cagtccctcc
     6901 cgcaaagaca gatgcgcaac caaatccatg atccccacca gggcgggaac agcatccggc
     6961 ggaacaagcc ctcgaaacac cacatgtttc tccagcccga gcgcgcgcac tcgtttctca
     7021 aagcgcccgc gccaggggcc atcgcccacg aacaggaacc ggatttgcgg gcaatggcgc
     7081 accagttcgg gggccacggt cagcaaatca tcgtggcctt tgagcttaaa gagccgcgct
     7141 atcttgccaa tgacgatatc atccggagcc aggccgaggc gggcgcgaag ttgaaggtcg
     7201 ttggcggccg ccaggaatgg agcgagcgca aagccgctaa ggatggttgt gtactgatcc
     7261 ggatgcccaa tgcccgccgc cagatattgc tgcttcatgg cctcggccac gacgacaaaa
     7321 tgagtggtga cgcgggcggc atgacgttcg gccgccttga agatcgaatt ggccagccac
     7381 ccttgaaagg aaccaaagga tgggccgtga atggtgtgaa ccacaattgg gacaccagcc
     7441 cgggcggcag ccaatcgtcc caataccccc gccttgctgc tatgagtatg cacgatctgg
     7501 ggggcttgca ctcgaaacag ccgcacgagc tcccgccaag ccatccagtc tttccagggg
     7561 tggaggggcc gaaccaacgg cggcacaatc gtcagcggga gagagttgtt agccaggaca
     7621 ctgtccagcg aaccttccgt accggtcgtt ggtcccgtaa tcagttggac gtccattccc
     7681 ggtttcgcgc gcaggcccag caccgaggaa accgtgtttt cctgggcgcc gccgatgatt
     7741 aatcgcgtga tgacgtgtag aatccgcatc cggggcggtt agcgcgcgcc gggtttcagt
     7801 tccatcaggc gagcgataac acttttggct tccacggtat tggtgggggc gttggccagg
     7861 tagagctcgt agtttctaat cgcagcgttg gtatcccgtt tgcgccaggc gatttcgccc
     7921 aggccatagg aaatctggga agcggtcgga aacactttct gcaggcgttc gtaatcgtct
     7981 ttagattcag ccagcttatt ggcgcgcagg taggcaatgg cgcgatttaa cagcgccgaa
     8041 tgattgttgg tttcaatggt cagcacctgc gtgagcgccg gaatcgcttt ttcaaaagcg
     8101 ccaacctgaa tacaggcgta gccctgattg atgagcgcgg tcacgttcgt gggggcaagg
     8161 attagctcct gctcgatgat gctgagcgca ttagagaaac gtccgtattt aagataaact
     8221 tgcgtcgccg tggccagaag gtccatgtcg ttgggatatt ggcgcaagga agcctggacg
     8281 accgcatccg ctccggcggg atcgtccttc cccagatgcg cggaggtttc gacaaaaagc
     8341 aattcggtcc gattggtgac agcggctgcc gccagatcgg gttgcgcgtg aatctcatca
     8401 atgatccgga gcgcctccgc cggcatcagg ctaacgacat ataactgccc caaccagatg
     8461 cgcgcgtcga gattgctggg ggccagcgac ttgacgcggt caaattgcgc ggctgcctga
     8521 cggtactggc tgttacgcac gaacacccgg ccctgctcga agcagaaatt tggctcatca
     8581 aacgggccat tttctccaat aacctgatcc cagttgcggt atttgccaaa ttcatcctcg
     8641 atggacttgg aaacttggac ggaagcgctg cgcccgactc gctggttccg attgcactca
     8701 aggttgatct gcgccacgat gttatccgga ttcaattcca gtgcgcgttc gaaatgcgcg
     8761 gcggcattgg tcaggcgacc ggtcttttga atttctaccc cccagtaatt cagcgcgcgg
     8821 gagtagaact tcgccacggt tgtggcatcc tgattgagtt cctgaaccag atgcgcgcgc
     8881 tttgccaatt tctccagcaa gccaggtttg ggaagggggt cggtttgcgc caccgaggtt
     8941 aacaggggag gaatcgcttc cgcgtttatt tttgcccaga aggactcgtt ttcagcaacc
     9001 agctttgcgt caaccggcgg cgcaaagaga tagttcgtgg aataaggctt aagctcgtaa
     9061 atcaatccgt gaggttccag gtgaaagaac tcaaagtaat atccaaagct gggatgcaga
     9121 taatagaggc tgtttgtctg agccaaacgg gaaaccaact gagccagcat taacggttcg
     9181 gcgagttgcg ctacttcccg gggaggattg ctttcccagg cttgcggata tttccgatgc
     9241 agaaaccggt gatactccgg ccactggagc gccgccgttt ctaaaaacat atgacgatta
     9301 ctttggccct tcctggcggc gtaggcttgc aacagatatg cccgacgcgg gtcgtcggtc
     9361 agcatgaaag agtgttgggc ggggagccct aatgccagcg cttccgcgta gcgtttaagt
     9421 gccggaccat tcgttgtgcg tatctgagtc cagtttctac aaatcagggc cagcggcgtc
     9481 agcacaagca ggagccaaat gaaaacggtg atggcgttgt tgagcaggcg caagtgggac
     9541 gactgtcggc ggggacgatc ggcggaacca ccgaataaaa gcaggaaata gccactgcaa
     9601 taaccgatgc agagcgcgcc caggtaatag aaagtgagga agggagcgcc aaagccccgg
     9661 ttgcgcggac taaagggcgg gtcgaacgcc acccagatgc aggccaggag aaacaggcca
     9721 tgcaccacat ggaacataaa agtggccagc cagactccca accggctgct gtcgccaaaa
     9781 tacgacgccc agcgaatcga gataataaaa aggggcacca acgaagtcag cgccagcaaa
     9841 gcgaccacct gccggccata cttgtaatat agcatcagga tgcttctttg cgcgcccagg
     9901 ttcgccttca gggcgggcca aaatccgacc gcgccggaat ctccgactac ttgcagaagg
     9961 ggcagcagca gatagagtaa caagcccgcc aggccgcaca aagacatgcg gccgagaaaa
    10021 cgcatattaa agaagcccag cccttttaac cagaccagcg ccatcaaaaa gaccgggaga
    10081 aagccgatca tagcccagtt cccggtcatc cccagtccga aagcgaaagc ggcgcgcatc
    10141 agccaggaat cacggtcatc caggcggaac tccaacaggc aacgaattac gtaggcaaat
    10201 accagcaagt cgagcatctc gcagccagtg ccccaaggca tggcaggagg cagaggctgg
    10261 gaagatgcgg ttgtggcgtg ctcccagaaa gtcagttgca gcccgcagac cagcaccgcc
    10321 agcactggtg gcagccaggc cgcagggatg gaaaggtaag gagatttatc tttctgtttc
    10381 tggcgctgct catgggtgcg gtcatgcggc agcaagacta ccgagcgggc cagcagggca
    10441 agcgttaggg ccgcgcaaac ggccgcaaaa aaattgaggg cgaggggaat ggcgtgcgag
    10501 ggcagccagc gaaacgggta ggtgaccagc cagaaaagcg ggccgtgtag ttccggctgc
    10561 cacgtccagc cgcaaagcct ggccacttgc ggaaggctgc ccagggatat ccaatgattg
    10621 agcgtcgcca ggtagagggc caaagccccc gcggcaacca gccagggcag aaatgtccgg
    10681 ataaaactct tctccggatt gacttcaggt tccgatgtca tgcggcaagc caaagttgca
    10741 tctatggccc ccgagttggc aagcttagtt tcatcctcgt gctgcgcggg gacgcttgat
    10801 gatatttatc tttgagccag ggggcggtag cgccagtttc cttccggcgc gcttcccggc
    10861 aatcaggggc tgcccgtcgc ggccttgcag cgtaaatccc ccacacattc cacatgttgg
    10921 gtatggggga acatatccaa cgggcgcacc tgggccaatt caaaggctcc ctgggcgcac
    10981 agaatgttca gatcacgagc catcgtcgcc gggtggcagg aaatgtaaat gatctgcgcc
    11041 ggttgcaccc ggcgcagcat ttgcaatgtt tccggctgac aacccttgcg cggcgggtca
    11101 agcagcacgg tggtggccgg tgcgggaaaa cgcgataata ttgctggcaa ctgttcttca
    11161 gcagttcccg cgataaattc gccgttggtg cgggcgcggg cggcggcatt tcggcgcgcc
    11221 gcctgaatgg ccatgtggtc caactccacc ccgacaaacg attcggccag cggtgcgagt
    11281 tccacactga agaacccaac cccgcagtaa atatccaaca ggtgttttgt ttgagcctgc
    11341 cgcagagcgt cgcgcgctac ttcgaccagc ttgggcagca ggaaggaatt gttctggaaa
    11401 aaggaatcgg gaggcacttc ccagcccggc ggcgaactgc gcaaaacaac cttgagcccc
    11461 cctttcggcg gggggtgcgc ccggacgtac ttgagctgct cgttaagggc tggctctgcc
    11521 aatcggcatt cttccacgtc caccaccagc cggctgtccg cccggataaa gccgatattc
    11581 agaccttgct tgaatttgtc ccattggctg cgaaccatga tgcgattccg atacccgtag
    11641 ggctgcgggc agggaataac cggcgcgacg agtctgcggt caaagtggcc caggcgctca
    11701 aacaaatccc cgatttgttt atgcttaagc cgaagctggg cggagtaagc caggtgctga
    11761 tactggcagc cgccgcacgc gccgaagtat cggcagccag gctcgacccg ttccggggaa
    11821 ggttccagca cccgcagcag ccgggcccgg gcatagcgct tcttcaactc cgttatttcc
    11881 gcttccacca gttcgcccac ggctacgaag gggacgaaaa ccacgaaatc ttcccagcgc
    11941 gccacacctt cgcccccgaa ggccagatcg ctgatccgca agctcagctt gttgcccagc
    12001 gccaaattca cgcttccgaa cggggccgga ctacctttcg gacttcagaa tccggtaatg
    12061 gccattctca ttctgctcga tcaggcgccg gttagtcagg gaaaccagcg gcgcgtctgc
    12121 ccattgggga ttctcctcaa aaaccgtccg tttaagggct ttccgggcaa tctctttccg
    12181 agcgacaaag cagtccggag tgccgcgcag aaattccatg atggctgttt cttcgtaggt
    12241 catgctccgc tggaaagcgc tccttcgggc gcagcctttt gcgagaaagc cagatggtca
    12301 ttctctgccg tgacaatgat cggttcgcct tcctgaaacg cgccgcggag gatttcctcg
    12361 gccagcgggt cttcgaggct gcgctcgacg gcgcggcgca tcgggcgtgc gccatacagc
    12421 ggatcgtagc ccttgctgac aaggaattcc cgggccttgt catccagttg cagcataatg
    12481 ttcttggcct taagccgctg catcaccttc gtgatttcca aatcgaggat ttgcaccaaa
    12541 tccggcttcg tcagcgcacg gaagacaatg atgtcatcca accggttcag gaattcgggg
    12601 cggaaggttt tcttagcctc atccaaaatc ttctcccgca tcttttcata gctgctctcg
    12661 tcagtgatcg gggaaaagcc gagcgtggac tgcttgcgaa tggtgtccga gcccacgttc
    12721 gaggtcatca ggatgatggt attgcggaaa ttgacaacgc gcccgacatt gtccgtcaac
    12781 ttgccttctt ccaggatctg cagcagcatg ttccacacgt cgggatgcgc cttctcgatc
    12841 tcatcgaaaa gcacgaccga gtaaggtttg cggcgcactt gttcggtaag ctggccgccc
    12901 tcctcgtagc ccacatagcc cgggggcgaa ccaatcaatc gggagacgtt gaatttctcc
    12961 atgtattcgc tcatatcgag ctgaataagc gccttggtat cgccaaacaa ctgttcggcc
    13021 agggttttgg cgagcaacgt cttgcccacg ccagtcggcc ccagcaacgc aaaggtgccg
    13081 attggccgcc gcgggtcttt gagatccgcg cgggagcgcc gcagcgcctt gcaaagggcc
    13141 gaaacggcct cgcgctggcc gataacgacc ttgtccattt cagcttcgac ctggagcagg
    13201 cgctgcattt cgccctgctc cattcgctgc agcggaatgc cggtccactt ggaaaccacc
    13261 tgcaggatgt cttcctcgtc caccttgacg cgcttttctt cccgcgtggt gcgccattca
    13321 ttcaacagcg cttccagctt ttctttcgcc tgcttttctt tgtcgcgcat ggaagcggcg
    13381 ccttcgaagt cctgatcttt gatcgccttt tccttccggg ccttgatttc ttcgatttcc
    13441 gcctcgaggc tcttgacttc cggcggccgg gtcatggtgc caattcgggc gcgggccccg
    13501 gcttcatcca tgacatcaat ggctttgtcg ggcagaaagc ggtcggtgat atagcgatcc
    13561 gagagcttga ccgaggcttc aatcgccttg tccgtgaact cggccttgtg gtgttcttcg
    13621 tacttcgggc gcaagccttt cagaatcaaa accgcttccg cgacggacgg cgcttccacc
    13681 ttgaccgcct gaaagcgccg ttccaacgcc gcatcttttt cgatgtattt gcgatactcg
    13741 ttaagagtgg tggcgccaat gcactgcaac tccccgcggc tgagggccgg cttgatgata
    13801 ttcgaggcat ccatggtgcc ctcggcggag cccgccccca cgatggtatg cagctcgtca
    13861 atgaaaagga tgatgtttcg ggagcggcga atctcgtcca tcacggcttt gatgcgctct
    13921 tcaaattgcc cgcggtattt ggtgccggcg accatcaacg ccaggtccag cgtaataagg
    13981 cgccggtcgc ggagcagttc gggcacgttg cccgccgcaa tctcctgcgc caggccctcg
    14041 acgatggcgg tcttgccaac gcccgcctca cccaggagca ccgggttatt cttcgtgcgg
    14101 cggcagagga tttgaatcac gcgctcgatt tcgctcttcc ggccgatgac cgggtccatt
    14161 tcccccttgc gggcgatctc cgtcaaatcg cggccgaaag ccttcagcgc gggcgtcttg
    14221 acctcgcctt tcttctcggc ggcggccttc ccggggatcg cttcgcccgg gatcggctca
    14281 tccgccgccg cgaagttggg atccagctcc ttcaggactt ccttgcgggt cagctcgata
    14341 tccacgtcca ggtttttgag cacgcgcgcc gcgaccccat cgccttcgcg gagcaggccc
    14401 agcagaatat gctcggtgcc cacgtaagta tggttcaggg ccttggcctc cttctgggcg
    14461 agcgcgagca ccttcttcac gcggggggtg taggggatgc tccccatcat cttttgatcg
    14521 gggcccgtgc caacctgctt ttccacttcc aggcgcaccg tgtccagatc gagccccagt
    14581 ttctgcagca cgttgacggc caccccctgg cccagcttga ttagccccag cagcaggtgc
    14641 tcggtgccga tgacattatg gttgaagcgg tcggcttcct tgcgcgccag cgccagcact
    14701 tgctgggcgc gcggcgtaaa attactcatg gcttcctgtt cgctcatata tcaaagtgcc
    14761 gttaatcggc gggtttgtcc aatccgcctt ccgccgccgc gggcggcggg atgaccgggc
    14821 ggctgatcag ccgcaggcgc tcccgcagca tatcggcgcg gagcaggtcc cgctcctcgg
    14881 tggaaagctt ttcggagtgt tgcttttgca agtgggccgg ctgggttaaa ataaacaatt
    14941 catccaccag cgcgcggtcc agctcgggga acaaccccgc gtcggacccc aggcgcatca
    15001 gggacaacag gttcatcgtt tccttggaag agatgctgtg ggcattggca aggatgccgt
    15061 aggcgcgccc gacatggttg taaaccagct tgggcttccg ctccaggagg ctggcgcggg
    15121 cattttcctc gtgctcgatg agctggttga ggaccttttc cagccgttga atgatggctg
    15181 cctccgactc gcccagggtc atctggttgg acacctggaa gatattaccc gacgcttcgg
    15241 tgccctcgcc ataaaggccc cgcaccgtca ggccaagctt gctgacggat tggatgatgg
    15301 gattgatttg ttccgccagc accagccccg gcaagtgcag cattgcgctc acgcggatgc
    15361 ccgtgcccag gttcgtcggg caggcggtca ggtagcccag ttccgggctg aaagcgtaat
    15421 cgagcttctt ttccaacgcg gaatcggccc cgttaataat ggcccaggcc tggtgcaact
    15481 ggagcccggg actcagggcc tgcatgcgca cgtggtcttc ctcgttgatc atgaaactca
    15541 gcgtttccgg gcggttcaag accagcccgc tgccggcgtt ccgggccgcg tgttcacggc
    15601 tgatgaggtg gcgctccacc aggatttgct tatccagggc ggacaagcga tccatggtcc
    15661 cgaagaaggc atcggccatt tccggcagcg cggcgatcgc gggctgaatg agctccagca
    15721 cgcgcagccg ttccgccttc ttggcccagc ccggaaaagc ggccttgcgg atattgcgcg
    15781 ccaggcgaac ccggctggac atgacaatcc ggtcgtgcgg gccgcggcgc cgcgcggttt
    15841 ccgccggggg aatcaaaaag gcgtcgagat tcataccggc cgctccgcgg cgtgctggct
    15901 caaccgggcc gtgacttgct tgatttcatc ccgcaggacg gccgcctcct cgaagttctc
    15961 caattcgatg gccttggcca gcttttgctg gagttgttta agttccgccg ccaggttgtg
    16021 cccctggcgc tgcgtttccg ggactttgcc gacgtgccgc gtgcctttat gcatcgtctg
    16081 gagcaccgct ttcaacggtt ccgcaaaagt ctcataacat tccgggcagc ccaggcggcc
    16141 ggccttccgg aaatctgatt gcgtgaaacc gcagaccggg caccgcagcc ccttttcgcc
    16201 gccggcctgc tcgatttcct gcgtcgcccc cagccccagc agtttatccg caaaactgaa
    16261 ccccgtgggg tcgtccatcc ccttggcctt ggcgcattct tcgcacagat tcaccttctg
    16321 catcttgtcc ccggcgatat tggtcagaaa gacggtcgct tcctttttct ggcaaatgct
    16381 gcacaacata cactcttcaa tcgtaaattg gctcgccgga aacctttgtc aaggcgcgat
    16441 actgcgccat caggccccgg gtcacccgtc ccggcttgcc gttgccaatc acccgcccat
    16501 ctattttgac gacgggcacc agctcggccg cggtcccggt cagaaaacat tcgtcggcat
    16561 tgaacaaatc atagcgcgtc aggttggatt caatcgcctt ccgccccgtt tccgcggcca
    16621 gttccatgac cacctggcgc gtgatgccgt agagggcgcc cgccgagagc ggcggcgtca
    16681 gcaaccgccc atccttgacg atgaacaggt tgtccccggt gcattccgcc acgaacccct
    16741 cggcgttcaa catgatcgcc tcctcgcagc cgccgttgtt cgcctcgatt ttagcgagaa
    16801 cattgttgag atagttcagg gacttgatgg ccgggttcaa ggcgctgtgc agattgcgcg
    16861 tcgtcggcac cgtgatgatt tccatcccgc gctcgtaaaa cgccgccggg tagagggtga
    16921 tcttccccgc aataatgatg accgaggggc gcttgcaccg gttggggtta agccccagcg
    16981 tgccaatgcc gcgggtcacg accaggcgga tgtacgcatc gcgcagccgg ttctgtcggc
    17041 aagtggcaat gatcgcggcg gtcagttccc gcgacgtcag cggaagccga agcaggattg
    17101 accgggccga gcaatgcagc cggtcaatat gctccttgag cttaaagatt cggccttggt
    17161 acgcgcggat tccctcgaac agtccgtccc cgtacagcag gccgtgatcg aacaccgaga
    17221 ttttggcgtc ccgctcgtta taaaacttgc cgtctatgta aatcttcatt tcacaaattc
    17281 aagttatgct acgcaatggc ggctgcgata gcaactcagg actcaggact cgccaatgct
    17341 tcggacctcc gggcggggcc ggttcccggc aatcgcgccg gcccgcgtgg cgccgctcca
    17401 cgcccggggc ctgggccgga gggcaggggg gcctgaatcc caattccatc cgcccgggtg
    17461 gccgcgggcg gggggcgaat tcacatcgcg tttccgccgg gtttcgcgta ttgtgccgga
    17521 gatgcgcctg ggcatattga ctttggcctc caacttgttt ctctcgccgc tggcgggcta
    17581 caccaacctg ccgttccggc tcaccgtgcg cgagttgggc ggcctgggcc tggcgaccac
    17641 cgacctggtc aacgcccgtt cgctgctgga aaagagggcc aaggccctca agttgattga
    17701 gacttccccg gcggaccgcc ccctggccgt gcaactgttt ggctccgtcc cggaggaaat
    17761 gcgcgatgcg gcggcctgcc tggaatcgct cggcgtggcc tccgtggacg tcaatatggg
    17821 ctgcccggcg cgcaaggtct gccgggttgg cggaggctcg accatgatga ccgagctggc
    17881 ccggacggcc cggctggtca aaggcatggt ggacgccgtc aggattcccg tcaccgccaa
    17941 gatgcggctg ggctgggacg aggaaaacct tacggccccg gaactggcgc gcgcgcttga
    18001 ggaggccggg gtcgcgggcg ttttcgtgca tggccgcacg cgcgcgcaag gcttccgcgg
    18061 ccaggtcaac ctcgcgggca ttcgcgccgt ggcgcaggcg gtgaaaaaga ttcccgtcat
    18121 cggcaatggc gacgtcacca ccccggaagc cgcccaaacg atgcttaacc aaaccggttg
    18181 cgccggggtc ggcattggcc gcggcgcctt ctacaatccg tggatcttcc agcatacgcg
    18241 gcactatctg cagaccggcc agcttctgcc cgaaccctcc ttcgaggagc gcgtgcgggt
    18301 gatgtgccgg catctggatc ggatgatcga ggtctttggc gaagcccacg gctgccggat
    18361 gttccgcaaa attgcgccgt ggcacgcccg gcggtttggc ccggcaaaag agtttaccca
    18421 tcgcgtggtg cgcgtttcca gccgcgccga atttctcgaa atcctcgaac gctaccgcat
    18481 ctggcgccgg cagttcctcg attcggacgg cgagttgctg ccgcgcttcc gcccgccgcc
    18541 cctggtggcg tcgttcctgc gctcacccga tcccgcccag gacggccagt tccccacgcc
    18601 caaagggccg gtcgaaatgt ggtagcgagg cagacctggc ggcggcccgc gcgactggcc
    18661 ggtccgccgg gaggcccgtg gcgcggcagg cacgctccag tttaaccggg gcgattcagt
    18721 ggaaaagcca atccatgcgt ccccgcgcca ctggagttcg atagggactt ggacgcccga
    18781 tccgcagcta caataaaccc tgtaattggc gcatttaacg gtcaggtcat tgagctccca
    18841 atgcctttcc actgtaaaat caatggttta gccattcagg agagaacaat agggagataa
    18901 agtctaaatc gccccggttt aggcatccct tggacatacc ttgcaaatac gccgtgtaca
    18961 aggggtgtac aacggatgct caccgggagt ccatcagatt ttagctggta cacccgatgg
    19021 tcatcccttg tacacccctt gtacacggcc tattggcagg gtaaatccgg gggacgttca
    19081 tggtgcgctg aagccgggat aattcaggtt ttggccctat tttgcccctc tttatgccta
    19141 aatccttgat tttaccggcg aaaggcttcg gattcggcag ctatgccctt gaattcgtta
    19201 aacaagaggt tctccagagc tacggtttgg atgctcagac caacccaggc ttcaatgaaa
    19261 cgagtaaggg cgattgaatc gtccaatgga gtcgtttcga gttagcgtgg ccttttcttg
    19321 cctaaagatg tcgaggaaac ggcagggcta ccatgaggcg gttttggcct cacgccatcc
    19381 cggccatctc ctttagattt cctatctatt ctaattctcc ggcgtgccta acctgctgat
    19441 tttacgggcg aaatagattg ttgaggcgac gatcccgatt gccatacccg cccaaacccc
    19501 gaaagaaagc tgtgtgtggg aatctggcga atgattttgc aggccgcggc tcgcggtatt
    19561 ttgccgcctg gttgcaggat acggaagccc tccaaggcgg cctcagtatc ggcggttccg
    19621 tcgcgcaggc gcgggcggcg aattggcact ttgcgcccat gaaaaacagc gttgtggccg
    19681 ctttagtggg gctgcccagg gccgggatgc tcctctccga cgcgccgccg cgcccaatgc
    19741 gggcttgcca ggggtaaagg tttccggtta agtgagcgtt aatggccaaa gttctcttgg
    19801 tggatgatga cttgaccacg ctgcaggtgg tcagcgagtt gctccgacag gaagggcacg
    19861 aggtattccc tttttccaac ggaaacgccg ccattgcggg gctgacggcg catgcgccgg
    19921 acctggtcat caccgacctt tactttgaca aaacgcgggc gcaagggctg gaggtggtgg
    19981 agaaagcgcg cgccttgtcg ccgccggcgg aagtgattgt cattaccggg ttcgggacga
    20041 tcgagacggc gctgcgggcg acgaagctgg gggcctacga ctacctggag aagccgttca
    20101 aagtggagga actcaagctg tgcgtgcagc gcacactttc ctacagcgcc gcccgaaacg
    20161 agaccgttca cctccggcgg cagctcaaga agaaatacgg gttcgatcaa atcgtgggca
    20221 gcgcgccgaa gatggtggaa gtgttcaaga tgatcgagcg ggtggccaat accgacagca
    20281 cgattttggt gctgggggag agcggcacgg gcaaggagct ggtggcccgg gcgctgcatt
    20341 acaacagccg gcggcagttt gccccgtttg tgccggtcaa ctgcagcgcg ctgccggaaa
    20401 acttgctcga atccgagctt tttggccact gccgcggggc gttcaccggg gcgatcgggg
    20461 acaagaaagg gctgttcgag gaagcggacg gaggcacgat tttcctggat gaagtgggtt
    20521 ccatgtcggc aatgctccaa agccggttgc tgcgggtctt gcaggagcgc gaagtccggc
    20581 gggtggggga aaaccggccg atttatgtca atgtccgcgt ggtggccgcc tccaacgaac
    20641 cgcttgagaa gcggatcaag gaaggctcct tccgcgaaga cctttattat cggctcaacg
    20701 tcattgccat cccgctgccc agcttgcgcg agcggcgcga ggatatcccg ctgctgacgg
    20761 cgcatttcct caaaggtaaa atggcgcccg ccgccagcca gccgctgcgg ataacccgac
    20821 aggcgatgga gatcctgcgc cggtacgact ggccgggaaa tgtgcgggag ctggagaacg
    20881 ccctcgaacg ggccgcggtg ctgtgcgagg gaggccttat tcaggccgcc gacctgccgc
    20941 cgcacctgct ggccgccgtc caggatgctg acctgatgcc cgagacccaa acggcgccgc
    21001 ctttgccggc ggtgccggat tcagcggtgt atccccttca tccccccggg gcgccggaaa
    21061 cggcgtcagc cgccgccgct cccggggcgg acgcgccgat tatcccgatg aaagcctact
    21121 tgcagcagca ggaactggcc tacctgaacc gcgcgatcca gcaatgcggc ggcaataagg
    21181 aacaggcggc cctccagctt gggataagcg tcgccacgct gtatcggagg ttgaatgccg
    21241 aggaaaagga gccatagagg tggaaatgga gccgcctttt ttgcttttaa tcccgggaaa
    21301 gccgtctagt attcgaggcg gttgcagtag ccgcgcctgg cggtggtcaa tgcaattcat
    21361 ggacgaagtg acttatgcca ttaacacata ctaaagaact gtttgcccgg gctttgaaag
    21421 gcaaatacgc gctgggcgcg tttaacgtga ataacatgga actgctccag gcgattgtcg
    21481 aagcctgcga ggaggaaaag gcgcccgtca tgctccagat ttccaagggc gcgcgcgaat
    21541 acgccaaccc cgtctatctt aagaagctca tcgaagccgc catcagcctg tccaatatcc
    21601 cggtggcggt tcaccttgac catggcgact cctttgaatt gtgcaaagac tgcatagatg
    21661 aggggttcac cagcgtcatg attgatgcca gccacgagcc gttcgagaag aatgtggcca
    21721 tctgccgcaa ggtcgtcgaa tacgcgcacg cccatgatgc ggtggtggaa ggtgaattgg
    21781 gctgcctgat cggggcgcaa tttgacgacg gcgaagaggg cggctcgttc tcgaaggagg
    21841 ggcgctatac caatcccgag caggcggtcg agttcgtgaa gctgtccggg gtggattccc
    21901 tggcggtcgc cattggcaac tcccacgggg cgtataaatt caagggcgaa cagcatctcg
    21961 acctggagcg gctcaaagcc attaagcagg cgttccagga cgccgggctg ggcgattacc
    22021 ccctggtgct gcatggcgct tccagcgtgc ccaaatccct ggttgatgaa atcaataaat
    22081 acggcggcaa attgacggat gccgccggtg tcccggaggg ggatatcgag gttgcgcgcc
    22141 gcctcggctg cgccaaggtc aatatagaca ccgacctccg cctggcgatg acggcggcca
    22201 tccgcaaggt tttcgccgag aatccccggg aatttgaccc ccgtaaatac cttggcccgg
    22261 cgcgggcgaa ggtcaaggaa ctggtgcagc acaaggtccg caatgtcctt tgctgcgcgg
    22321 gccatgcctt tgactagtcg ttagccccgg ccgtttctcg gcacgtcaaa atgatgaact
    22381 acgaagcatt ctacaagatc agctacggac tttacgtggt cagctcggcc catggcggcc
    22441 aaaggagcgg ctatgtaggc aacacggtct tccaggtcac ctcccagccc gcgcgctttg
    22501 ccatcgcttg caataaggat aattttacgt gcgagctgat ccgccaaagc caggggtttg
    22561 ccttttccat cctgcagcag gatacccggg cggagctgat cgggttgttt ggctataaat
    22621 ccggccgggc cgtggataaa ttcgcctcgg tgcgccatcg aacaggccag accggtgtgc
    22681 ccatactgct ggaggacgcg ctggcctggt tcgagtgcaa agtggtgcaa accctggatg
    22741 tgggcaccca cttgctgttt ataggcgagg tggtggcggg cgaactcatc agcgacgcgc
    22801 aacccccgct gacctacgcc tactaccgcg aggtcaaaaa gggcaaagcg cccaagaatg
    22861 cccctaccta tatcccgccg gccagcacgg ctgccgcccc gccggcggcc gcagcgcccg
    22921 ccggcaaata cgtttgcacc gtttgccatt ttgtttatga cccggaacag ggcgacccgc
    22981 acgccggcat cccggcgggc acccgcttcg aggacctgcc cgaggggtgg gtctgcccgg
    23041 tctgcggcgc ccccaaatcg gactttgtcc cccaaacctg acttgaccct ggagcctact
    23101 cgatcactgg tatgaagacg cctgaaattg tgcagaaatc ccccgcggtt cagaaggttg
    23161 agccgggaac gtactattgg tgtgcctgcg ggaaatccaa aacgcagcct ttctgcgacg
    23221 gttcccatcg gggaacggag ttcgctcccg tgcgggtgga gatcagcgaa gccaagacgg
    23281 tcgcctggtg cctctgcaag cacaccaaaa ccccgccgtt ctgcgacgga acgcaccagc
    23341 aactctagcc cccggcagcc gcttccaatc gaggatcgcg cgtgaaagct acggcttgaa
    23401 aaccgggtag cttccagtca gactcaagcc gtgatagacg gacgcaaaac cagctttccc
    23461 ttgtcggccg cctcgccggg cgcctgtggg tgtatgcggg cgcaaattat tcccgcggga
    23521 ggactgcgtt gcctcgtccg cgtggtggga gtcgctttcg ggattgcggc tggcgtctgg
    23581 ctggcgggcg catcgtgtgg agccgccccc gcaaaaacca cggcgaaagc ggtgccggtg
    23641 ccgcccacgg cttcggtcaa tccgttctac cccgggaacc gggagccgtt gttgcccagc
    23701 ccgctgatca agctgccgat cgggagcatc cagccccgcg gctggctgcg gcatcagctc
    23761 gaattggaag cgcaagggat gacgggccgg ctgccggaga tatcgaagtg gtgcaagttc
    23821 gagggcaatg cgtgggccga cccgcagggc cggggccaca gcagttggga ggaactgccg
    23881 tattggctca agggctacgg cgacctgggc tacgtgctgg gcgatccggt gattactcaa
    23941 aacgcccgga aatgggttga ggcggtgctg gccagtcagc aggcggacgg ctggttcggc
    24001 ccgatcgcca acaagaccgg cctgaaaggc aagccggacc tgtggccgca catggtgatg
    24061 tgcaacgtat tacagtcctt ttacgagtat agcggcgatg cccgggtgct gccgttcctc
    24121 acgaaatact tccgctggct gaacaccctg ccggacgagt gcttcggcgc gggctattgg
    24181 ccccgcatcc ggtttggcga caatgttgaa accttgcagt ggctttacaa ccgcacgggc
    24241 gagccctggc tgctggaact ggcccggcgg attcatgaga acatggagga ctggacccgc
    24301 ggcgtgcata actggcataa cgtgaacctg gcgcaaggct ttcgcgcccc gggcgtgtat
    24361 tatcagcagg ccaaagaacc caggtttctg gccgcggtcg aacgcaatta ccagacgatc
    24421 atggagcgct atggccaatt cccgggcggc ggctttgccg gagacgagaa ttgccgcccc
    24481 ggctacaccg acccgcggca gggcttcgag acctgcggca tcgtcgagtt tatgcacagc
    24541 ttcgagctgc tgacgaaaat ctcgggcaac ccgctgtggg ccgaccgctg cgaggagatc
    24601 gccttcaact ccctgccggc gtcgctgacg ccggactgga aagggctgca ctacctgacc
    24661 tgcccgaacc aaatccagct cgataaggag aacaaggcgc ccgccatcca aaacaaaggc
    24721 accatgttct cctacagccc gttcgagcgc taccggtgct gccagcataa cgtgtcgcac
    24781 ggctggccct attacgccga ggaactctgg ctggcaaccg ccgaccgcgg attatgcgcc
    24841 tccctttacg ccgcctgcca ggtgaccgcg caggtgggcg agggaacgcg ggtgacgatt
    24901 gcggaagcga ctgattatcc ttttggcgac acggtgacgc tgcgcctttc catgccgcaa
    24961 gcggtcagtt tccccctgta tctgcgcatc ccccggtggt gcgcgcgagc ggcgataagc
    25021 gtcaacggcc ggccggttcg ggtggccgcg ccgcccctgc actacgtcgt gctggagcgc
    25081 gcctggcagg atggcgatac ggtgagcctg cgcttgccga tgcaggtggc cgtgcggcgc
    25141 tgggagcaga accagaacgc cgcttccgtg gattacggcc cgctgacctt ctcgctcaag
    25201 ataggcgagc ggtggtcgcg ttgcggcggc acggacgcct ggccggaact ggaagtgttt
    25261 cctaccacgc cgtggaacta cggtttggtg cttgacccga agtgcccggc ccggtctttc
    25321 aaggtggtgc gcgggccccg aagcgccggg ctggcggggc agccgtttga accggcagcc
    25381 gcacccattg aactgcgggt tcaggcccgg cggatccctg actggaagca ggatgcgctc
    25441 ggcctggtgg gcaagctccc cctcagcccg gtgcgctccc gcgagccggt ggaatccatc
    25501 accctgatcc ccatgggcgc ggcgcggttg cgcatcacgt ccttcccggt catcgcgtca
    25561 ggaaagattg cggaatagcg agagaacgat atgattagcc ttacccttat gaaacaaaca
    25621 cgcctgtatc agcgaaatcc attggccgcc gggatctgcc tggcactgtt attcctgacc
    25681 ccggctccag cgcgcgacgc ctggcagccg gccgctgggc cactcaagac gcggtgggcc
    25741 aaagaagtct caccccacaa ggttcacccc gaatatccgc ggccgcaaat ggtgcggaaa
    25801 gactggctca acctcaacgg cctctgggac ctggcggtca ccggcaagat ggcggcgcgg
    25861 gccaacttcc aggagcgcat cctggtgccg ttcccgatcg aatcggccct gtccggtatc
    25921 ggccgcccgg tcagtgaaaa cgatcgcctc tggtatcggc gcacctttac gctgcctccc
    25981 caatggcagg ggcggcgcat cctgctccat ttcggcgcgg tggatttcga cacgactgtg
    26041 ctggtcaatg gccggaaggt gggccaccac cgcggcggct atgacgcctt tagtttcgat
    26101 atcacggatg atgtccggcc ccacggcgag aatgagctga tcgtgtcggt ctgggacccc
    26161 accgacgccg gcacacaacc catcggcaag caagtgcgcc agccgcgtgg catctggtac
    26221 accgccacca gcggcatctg gcagacggtg tggctggagc cggttcatgc cgtccatatc
    26281 tcgggcctga aaatcacccc ggatgtggac gccggggcgg tcaccgtcga ggccatcacg
    26341 ccgccgctgc tcgccacttg cacgctggaa atgaccattc gcgaccgggg caaggtggtg
    26401 catgccgcca gcctttccgg cggacggcag atcaccatgc cggtcaaaaa cgcccgcctt
    26461 tggtcgccgg aagacccgca tttatacagc ctggatgtcc gcctgaaaat cggccgccgc
    26521 accctcgacc gggtcgaaag ctactttggc atgcgcaaga tttcgctggg caaggacgcc
    26581 cggggcttca cccgcctcct gctgaacaac aagccctact tccaattcgg cctgctggac
    26641 cagggattct ggcccgacgg cctgtacacc gcccccaccg atgcggcgct gcgctacgat
    26701 atcgagatga cgaaaaagct cggcttcaac ctggcccgca agcacgtcaa gattgaaccc
    26761 gaacgctggt attactggtg cgacaagctc ggcctgctgg tgtggcagga tatgcccagc
    26821 ggcgaaaaac acatcgggcc gaaagatccg gacatcaccc gcagcccgga atccgccgcg
    26881 caattcgagc aggaactcac cgccatggtt cgcggacgct acaaccatcc ctgcatcgtc
    26941 atgtgggtgc cctacaacga gggctgggga caatgggaca ccgcccgcat agtcgagttg
    27001 gtccggaaac tggatgccac ccgcccggtt aataacgcca gcggctggac cgaccgcggc
    27061 gtcggcgacg tcaacgacct gcataaatac cccggcccgg gatcgcccga gccggaggcc
    27121 aaccgggccg tggtgctggg cgagttcggc ggcctcggcc tgcctatccg cggccacacc
    27181 tggcagagcg agaagaactg gggctaccgc agcttcaccg actcggagac gctgaccgac
    27241 gcctacattg acctcgtcga aaaactcttc ccgctcattg acgagaaagg cctttccgcc
    27301 gccgtctata cccagaccac cgacgtggaa attgaagtca acggcctgtt gacctacgac
    27361 cgcgagcggg tgaaaatgga cgcccggaaa atcaccgccg ccaaccgcgg ccagttcctc
    27421 ccgcggcccc gcgtgacgga ggtggtgccg accgcgctga ccgagcggcc cgcctggcgt
    27481 ttcacactcg aaaaacctgc cgctgactgg caccagcccg gattcgacgc cggcgcatgg
    27541 aaggagggcc aggccggttt cggcacgaaa ggaacccccg gcgcggtcgt gcgcacggag
    27601 tggaacacca gtgatatttg gctgcgccgc gcgttcgatc tcccggcggg caaatcggac
    27661 gacctgcgcc tgctcattca ccacgacgaa gacgcccagg tatatttcaa cggagttctg
    27721 gcggcgagcg ttccaggcta cacctcggat tacgggcaag tccgcatcag cccggaggcc
    27781 cgggccgccc tcaagcccac cggcaatatc atggccatcc attgccgcca gaccggcggg
    27841 ggccagtaca ttgacgcggg catcgtgaag attgagcttc ctaaaaactg atgcgtcggc
    27901 gagccctcct gctcctgctc tgcgcggcga atctcgccac cgccgcagcc aggccggatc
    27961 actgctcggt gcgcgatttc ggggccaaag gcgacggcca cactgacgac acggcggcgt
    28021 ttcagaaggc gctcgataaa atgggccggg ccggcggagg cgtggtttat gccccgcgcg
    28081 gcaattattt cttcgccggc cacctcaccg tgcccgtcgc cgtgacgctc aaaggcgtct
    28141 gggaatccgt ccccgcccat gtgggccttc gcaacccggg ctggaacaag cccaccgacg
    28201 atggcacgac cttcctcgtc acggagaacc ggggccggga ggacggcccg cctttcatca
    28261 cgctgaatgc ggacgccacg ctgaaaggcg tggtgatcta ttatccgggg cagaacccgg
    28321 aggtggagcc ggaaccctat ccctatgcca tcgcgctgcg cgggaaaaac cccgccgtgc
    28381 tcgccgtcga gctgctcaat ccctacaacg ggattgacgc cacccgcaac gagcggcacc
    28441 tcatccgcga cgtgcacggc cagccgctgc ggcgcggcat cctggtggat gacatctatg
    28501 acatcgggcg catcgaaaac gtgcatttca atccctggtg gagcaccaaa ccgcgcctgc
    28561 gccagtggct gcagcagcat ggcgaggcct ttatcttcgg ccgcagcgac tggcaataca
    28621 ccctgaacac cttctgcttc ggctaccaca tcggctataa gttcatccga accgccaccg
    28681 gcctgtgcaa cggcaacttc ctcggcatcg gcgcggacga ttgccacact gccctgttcg
    28741 tggagaactg cgcgcccatg gggctgttga tctccaacgg cgagtttgtt tccttccacg
    28801 gcccggaccc gacgatggtg cgcgttggca ccaacaacgt gggcagtgtg cgcttcgtca
    28861 actgcgcctt ctggggcccc tgctgccaga tcgccaccgt cgccggcaag ggcacggtgg
    28921 gtttcagcga ttgcaccttc gtgcagtggg accgggagaa cgaggggcgg cccgcgctgc
    28981 aaatcatgag cggcaccgcg ctcgtccggg gatgcgagtt ccaggccgat aaaccgcaga
    29041 tcgaactggg caaggaagtc cggcgcgccg tcatcacgga caacctcatc accggcatgc
    29101 tccgcatcac caaccacagc ccggccaccg taatcatccg ccacaacgcc ggcgattccc
    29161 ccggccagcc gcaggaatga tgacggcccg ccgttttgcc ctaaccgcgg ggctggccgc
    29221 ctcgctgctg ggttgcgccc ggcccgcgac caaacccgcc gccccggccg gcctgccgcc
    29281 gccggctccc ggcgtcttct tcgtcgccac caatggccac gaccactggt ccggccagcg
    29341 cccccggccg aatcgccaac ggacggatgg cccgttcgcc acgctgcccc gcgccgtgca
    29401 ggcggcccgc gcctttcgcc agcaaagcgc cagccccgcc gcgcggccgg caagcatctt
    29461 cgtgggaggc ggcacgcatt tcctggcgca acccctcgcg ttgacccccg cggactccgg
    29521 cctgacgctc gccgcttacc ccgacacgca accggtcttg agcggtggcc ggcccatttc
    29581 cggctggcgc gaagtcacga tcgccggccg gcgattgtgg tgcgccgacc ttcccgcgga
    29641 actgcgcggc caatggtcgt tccagcagct ttgggtcaac ggccagcggg ccacccgcgc
    29701 ccgccatccc aaccacggct acctcgccgt cgccgggctc cccgacaaaa ccaaggattg
    29761 gacgcaaggc catagccgct tccggtttca ccccggcgac ttgccggccg gagaaaccat
    29821 cacccaggct gaggtggtgg tgatgacgcg ctgggtcgag tcgcgcctgc cgattgccgg
    29881 cgtggacgaa aaaagacgcc tggccacctt cggcaagcgc tccgtcttcg cgctggcgtc
    29941 cggcgacctc tactatgcgg aaggcgcctt cgagttcctg gacgaccccg gcgaatggca
    30001 tctcgatcgg acccgcggcc ggctgttcta cctgccgcgc cccggcgaga cgctggctcg
    30061 agcccaggca atcgcgccca tgctggcgca ggtgcttcgg ttcgagggcc gccccgaagc
    30121 cgggcagttc atcgagcgca tctcgctgcg ggggctgacc ttctcccaca ccgaatggca
    30181 tttcccctcc gaattgccct ccgccgcgga gcagccgaag gtctggcccc ctccggcgac
    30241 cgaggtcggc ggcttcgccc aggccgccgt tggcgtttcc ggcgccgtgt atggggaggg
    30301 gcttcgccac tgtcttttcg cggactgccg gttcgcggcc ctcggcaact atggccttga
    30361 actgagccgc ggctgccagt ccaaccacat cgcccgctgc gaattcaccg acctgggggc
    30421 cggcggcctc aagctgggcg aaacccgcat ccgcgaccag gcggccgaac agacccgcgc
    30481 caacgaaatc agccattgcc acctccacga cggcggcaag ctgtttgcca gcgccgtcgg
    30541 catctggctc ggccaatccc ccgacaaccg gattctccat aaccacatcc acgactttta
    30601 ctacaccggc atctccatcg gttggacctg gggctacggc cccgccctcg ccggcaataa
    30661 cctcgttgcg ctcaaccacg tccaccacct cggccagcgc tccaatggcg acggccccat
    30721 cctcagcgac atgggcggca tctacaccct gggcaaacaa cccggcacca ccatccgcag
    30781 caacctctgg cacgacatca gcggcttccg ctacggcggc tggggcatct acttcgacga
    30841 aggcagcagc ggcatcctgg ccgaagacaa cctcgtttat cgcaccaccc acggcggttt
    30901 ccatcagcac tacggcgaga ccaacctcgt ccgcaacaac ctcttcgcct tcgcccgcaa
    30961 ccaccagctt cagcgcaccc gcccggagcc gcaccggagc ttctccttcg tcaccaatat
    31021 cgtctatttc gactccggca acctgcttgg cggcgactgg tcgaacgacc agtacctgat
    31081 ggacgcgaac ctctactttg acgcccgccc cgacgcccag ccggatcagc tccgcctggg
    31141 cccctgcacc tggcaacaat ggcaggaacg cggccatgac cgccattcgc tcgtcgccaa
    31201 cccgctctgc gtcgcccccg acaaggacga cttccgcctc caacccgcct ccccggcctt
    31261 ccgcctcggt ttccgcccca ccgacctgtc aaacgtcggc ccttgacccg caccaactga
    31321 ggacggtttc gattcccggc gggggccatc ccgcaagcgc gcatgcgctt gcaatcggca
    31381 tcccctcctg ccaaccccct gattgcaagc attgggccgc accctggccc cagccgccaa
    31441 tgccgcttgc gcccgccgac gcctgcggct gattccacct tccccgattt ctcccgcctc
    31501 cgtagccctc atagggctcc caataggccg tgtacaaggg gtgtacaagg gatgctcacc
    31561 ggatgcccac gctttaaccc catgtttctc ggttgagcat ccgatgtaca ccccttgtac
    31621 acggcttgaa atgagggagg tgcgagcatc cgtggacctt ggaattatca gggggcttgg
    31681 tattataact ttcgtttttt cagctatttg gagattttga gactaaagtg atagcgggat
    31741 gttggcatta agaggctgat tttggctttc cagccgttcc cggccggggt cttggcaagg
    31801 ggttattttt cggattgtcg gcttgattta gctctcgtgc ggggctagtc ttgtgggagt
    31861 tcaaagccaa atcaaagact tcggcaggcg cttttcgata ggcgtcatgc tgctaaacgc
    31921 atgaaaatga attatttcgg aatcgtcttc gcgctgctgt tgggcagtat ggtttcatcc
    31981 ccggccaccc aggaagggcg ggatttgccg gaaacgcctc atcccgaacc ggtgcggggc
    32041 ccggcaccgg ataatatgcc ggtgaagggc gctttgccgg gccaaagtgg cgtgcccaag
    32101 gctttgctgg ggccgattgc gccggccggg gcgcagccca caggggcact gtcgggccgg
    32161 attgtgttta ccagcggcgg gcatgggctg gcgtgggatg gggcgagttg gacgacgggg
    32221 cgcggggtga actgggagat ggtggaggac tacgggaacg tggatcaaat gagcatgttt
    32281 gcctactact gcttcaatgc gggggcgacg gtggtggcga tgcgcccgat tggcaatcaa
    32341 accaacgagg tggttttgga caatgttgac ccggaggtga ctttccaggg tgcctgggcg
    32401 gacagcgtct ttaccaatta ttatggcaat gccggggacg tgccgtatcg cttcacctcg
    32461 gttgccgcga cggaaacggc gacggcgacg tatgtgccga acatcccggt tgccgggttt
    32521 tacccggtgt acacctgggt ctggcattcg accaatcgca ccagccagct ttaccgggtg
    32581 cggcatacgg gcggcgaatc gcaggtgcgc gtgccgcatt acctggtggg cgggggatgg
    32641 gtgtacctgg gcacttatta tttcgcggcg ggctccgacg cggcccgcgg cgcggtggtg
    32701 atcagcaacc tggcgccgag tccaggcgtc gggagcgcgg tgatcgccga tgcgattcgc
    32761 ttcggcaatg gcatgggctc gatcgcccgc gggggcggcg tctcgggcca cccgcgggag
    32821 catgaatgcg cccgttattg gattcaaagc agcctggggc ggggctcgcc cacctggatt
    32881 tatgacgacc cgtccttgat tgattccgac gataacgtga gcgcgccgat tcgcatggcg
    32941 cgcgagatga acgaggaggc tgcgggcaat ttctatcagc ggatttacat cgggtttcac
    33001 tcgaacgcct cgggcctggg gaccaacagc agcgcgcgcg gcgacatcgg gctctataac
    33061 aacgacaacc tgttccccgg gacagcgact tccaaccagt tccgcctggc ggagatcatc
    33121 gccaccaacg tgaacaatgc cttgaagcgg atcaccgtgc cgccgttcga ggtcccctgg
    33181 ctcaacaacc gttcgagcct gacgtatgcg cggactgatt tcgcgtttgg ggaaatccgc
    33241 ggcgaccgcc ttggctacga gatggacgcg acgattatcg aggtggcctt tcacgacaac
    33301 gtctccgatg cgcggctgat gcgcgatccc aaggttcgca attggatcgc gcgggcgtcg
    33361 taccaggcgg tggtccacta tatgcaccag tttgacgcgg tgccgctgaa ttttctgccg
    33421 gaaccgccgc gccatgtgcg cgcggtggcg acggccaatg gcattcaggt ccgctgggat
    33481 gctccggtgg cggagttctc gacgggaaca ccggccgggt ttgtggtgta tcggtccacg
    33541 gatggctacg ggtttgggga gccggtcgag gtcgcgggcg ggggcgcctc gatgattgag
    33601 ttcaccaatg tcctggccga caccgattac tacttccggg tggcggcggt gaacgcgggc
    33661 ggggaatcct tcccctcgga gaccgtcggc tgtcggcgcg cctcgaatcc ggcgcattcg
    33721 cgcgtgctgt ttgtgaacgg gtttgaccgc ttcgaccgca ccacgaatct gcggcagaat
    33781 cccacggggg cctatgcgcc tccgggcaat tccggggcca tggagcgcgt gatcccgcgg
    33841 agcaacaact cgtttgacta tgtggtgccg catgggaagg cgattagcgc ggccgggctg
    33901 ccgtttgatt cctgttcgcg gccgtcggtg accaaccatc aggtggcgct gaccaattac
    33961 gacatcgtga tctgggcctg cggccagagc ctgaccaaca ccttccgcgc gccggagcgc
    34021 aatgtcgtga cggccttcca gaacggcggg gggcacttgt tcctcaccgg gtcggaagtg
    34081 gcgtgggacc tggatcgggc agcggggccg tccgccgcgg atcgggcgtt cctcaataac
    34141 cagctccatg ccgatttggg gccggacgcg aacaataatt cttcgaacta cacctttgcc
    34201 gcggtggcgg gaacgatttt cgaggggaac agcggcggtg cgttcgacga cggcaaccag
    34261 gggatttact gggtgaagac gccggatgtg ttggtgccca ccggcagcgg cgcaacggcg
    34321 gcattggtct atggcggggg cgggggcaag gtggcgggga tccagtatgc cggcgcttcg
    34381 ggcgggaggg tggtttactg gggattcccg tttgaaaccg tgaccgacga agcgattcgc
    34441 gccgactata tggcggatgt gctccgcttt ttcgcccggc cgccgcgatt cgaacgggtc
    34501 agtttcggcg cggggccatc ggtgcggctg tggttgaatg gcgacccggg tgaatatacc
    34561 ctccaaacca gccctgacct gattaactgg gcgttttgga ccaatgtgca gatggggctg
    34621 cagcccatcg aaatctctga cgactccttt accagcgcgc cgcgccgttt ctatcgcgcc
    34681 ctgcgataaa accggcgggt ttcaaagcgc tcgacccggg atgggcggaa gttcacgcgc
    34741 gcctcggccg ttatgctttg cttgcgggag ttggaatggc gggaatgttg gtcttcgcaa
    34801 acggcgggac ttcgagatgc cctgtgaggt tcaaccgaac cgggtcggga gcgccgcgcc
    34861 cggggcgcgc ttgccagttg gccggccaat tcgatacttg catatacggg gttttaatct
    34921 aaaaggacat gaatgttagg tcgtaattta cctccctggc gcggtgggct ggccctggcg
    34981 ttgagcctgc taatgatgat cgcctctgga gacgccggcg cccggagccg cccggaggtt
    35041 cgggaattga atccgcagat cgaggcggcc attgccaaag tgaaaccggc cctggtccgg
    35101 attcgcgtcg tggagaccga ctacagcgag gggcgggaga ccaagtcgca ggcggtcggc
    35161 agcggagtga tcattacccg cgatggttac ctggtgacca atcatcatgt ggcgggacat
    35221 gcggtgcgca tcttttgcac cctgtggaat cgcgaagaaa tcgaggcgga actggtcggc
    35281 accgacgccc ttaccgacat cgcggtgctt cggctgacac cagcgcgccc ggtcgagttc
    35341 aaaacggcca gctttgggga ttcctcgaag gttcaggccg gggactacgt gctggcgatg
    35401 ggcagcccga tggctttgtc gcaatcggtg acgctgggca ttatcagcaa cccggaaatg
    35461 atcatgcccc ggtcgggaag gggcgaacgg gctttccggc tggatggcga agatgtgggt
    35521 tcgttggttc gctggctggg gcatgatgcg gccatttacg gcggcaactc gggcgggccg
    35581 ctggtcaacc tgcgcggcga gatcatcggc attaatgaga tcagctatgg cctggggggc
    35641 gctattcccg gcaatctcgc ccggagcgtg gccgagcaga tcatggccaa aggccgggtt
    35701 gtgcggagct ggctggggct ggatgtgcag ccgcggttca agcgctcgac ggaaccgcat
    35761 ggtgttttga ttagcggcgt gcaggcgggg tcgccggccg accaggcggg attcaagccc
    35821 ggcgatctgt tgctgcggtt ggggggggag gcaacggatg tccggttcga cgagcaaatg
    35881 ccggatttta tgctgctggc caccagcctg cccctcgggc aggaaacccg ggcggtggtt
    35941 aagcgcaacg gcaaggaact caccctccgc ccggtgccca tcgagcgcgg ggaggtgtat
    36001 cccccgcagc aggaactcaa ggcctggggg ttgacggtgc gggacctttc gcgcctgctg
    36061 gcccaggaga tgaagcgagc cgacccggtg ggggcgctgg tgacttccgt gcgcccgggc
    36121 gggcccgccg gcactgccaa gcccgcgctc caagcgaagg acgtgatcgt ggcggtgaat
    36181 gatcaaccgg tgcgaagcac ccgggacctg gtcgcgctga cccgcaagct gactgaaggc
    36241 cggacggagc cgttgccggt catggcaacg ttcgagcgcc gggccgcgcg gcttttggcg
    36301 atggtgcgga ttgggctcga cgaacttaaa gacccgggcc tggaagtcac gaaagcgtgg
    36361 ctgccggtcg agacgcaggt cattagccgc gagatagccc ggcaactggg ccagcccgct
    36421 ttgaagggct tttatatcac gcgcgtctat cccggcacca ccgccgagca ggcggggctc
    36481 aaggccggcg acttcatcac cgcggtggac ggcgagaagc tgtcggcctc cgactccgag
    36541 catgaagacg agctgtccgc gctgatccgg caatacgacg tcggccagac ggtgacgctc
    36601 acgatccggc gcggccaggc ggagcacaaa ctgccggtct cattggcccg ctcgccgcgc
    36661 ctgcagcgcg agatgcggaa ataccgcaac gaggatttcg agtttactgc gcgcgacgtg
    36721 tcgtttttcg atgtggtcga agagcagtgg agccgcgacc agcttggcgt gctggttgag
    36781 gaggtcaaac ccggcagttg ggccgagctc ggttcgctgt ttaagggcga cttgatcctc
    36841 gaagtggccg gccagccggt ggacaaggtg gacgcgctcc ggcaggagct cgagcgtgcc
    36901 gccgcccgcc gggatcaggt ggtgcggttc aaagtgctgc ggggcattca tactacctac
    36961 ctggaaattg aaccaaactg gaaaaactaa cctctaatcc caaagcttat atgcgatatc
    37021 cctattccat tcttcgggcg gccctggccc tggcgctggc cggcgcggct tgctcccttc
    37081 gtgccgatga actgggcgac aaaggccggg acatcttccg gaaataccag cagacggtcg
    37141 tcacggtgca ggtggtgcag aaaattacct actccggcgc cgccaaatcg ggggaaaacc
    37201 ggcaggaact caccggcacg gtggtggacc cgtcgggtct gaccgtgctg gcgttgtcgg
    37261 cgtgcgatcc cagcgaaatg taccagcgca tgctcgcgca acaaggctcc cagaacaagc
    37321 tggagatcga ggtggccgat atgcagatat tgctggcgga tggcaccgag ttggccgccg
    37381 agattgtgct gcgggacaag gatctggatc tggcctttgt ccggcccaag tccaaacccg
    37441 ccacgccgat gaccgcggtg gatttggccc ggtcctcgac ggtgcagatt cttgaccagg
    37501 tcatcaccct taaccgcctc aatagcgccg ccggacgcgc ctatgccgct tccgtcgagc
    37561 ggatcaacgc cgttatccag aagccgcgca ctttctacgt gcccgacagc accatgactt
    37621 ccaccaccct ggggtcgccg gcgtttgccc tcgatggcaa tatcgttggg gtggtggtga
    37681 tgcgggccgt tgcgtccggg ggaggcgcca gccgcagcgt ccgcgaaaat ctgagcgcga
    37741 ttatcctccc ggcagaagac atcctgaaag ctgccaagca ggccccggaa gccaaaggtg
    37801 agccggggaa gaaagaagaa gcaaaagacg cccagccggg caagtagccg gtcgaagaaa
    37861 aggcggggcg cccccgaagt accggggata gagggcattc tttgggccgg cttgccgatc
    37921 cggggcggcg ctgcggagcg cggagtggcg ggatggcggg gacggattta cgcgtgcata
    37981 ggcgggcctt tttgccgtaa gctcccggtg tggaatccag cgtcccgcaa ctgctgccgc
    38041 tgctgaagca atacttcggc ttccgatcct tccgcccgct ccaggaggag atcatccgcg
    38101 atgtgctggc gggccaagac gtgttcgcgc tgctgccgac cggcggcggc aagtcgcttt
    38161 gctttcagtt gccggccatg gcgcggcccg ggctggcggt ggtggtatcg ccgctgatcg
    38221 cgctgatgaa ggaccaggtg gacgccttgc aggccagcgg cgtgccggcg accttcctga
    38281 actcgtcgct ggcggcgggg gaatcgcggg cgcggctgcg cgggctgcat aacggagagt
    38341 tccggctgct ctacgttgcc ccggagcggc tgatgttgcc gggatgcctc gctgacctgc
    38401 agcgatgggg ggtgaacctg ctggccattg acgaggcgca ctgcatcagc gaatgggggc
    38461 atgattttcg gccggaatac cgccagcttg ccgagctgcg ccagctattt cccaccgtgc
    38521 cgatgatggc gctgacggcg acggccaccg cgcgggtgcg ccatgacatc gtgacgctgc
    38581 tcaggctgcg cgaaccccgt tgctatgtcg ccagcttcaa ccgcccgaac ctgacttacc
    38641 gggtgttcgc caagcataag ccctatgatc agcttcggaa gttcctgcgc gagcgccccg
    38701 gccagagcgg cattgtctat tgccaggcgc gcaagacggc cgagagcgtc gcgagccgcc
    38761 tcgaagcgga cggtttgaaa gccaaaccgt accatgcggg tctcacggcc aaagagcgga
    38821 cggcgcacca ggagcttttc ctgcaggacg aggtgcgggt gatttgcgcg acgattgcgt
    38881 ttggcatggg catcaacaag cccaatgtgc gtttcgtggt gcactatgat ttgccgaaga
    38941 atattgaggg atactaccag gagaccggcc gcgccgggcg ggacgggctg cccagcgagt
    39001 gcgtgctgct tttcagcgcg ggggacgtgg ccaagctggt gcggttcatt gacgaaaagt
    39061 ccgacccggg agaacggaat atcgcgcacg agcaactgcg ccagatggtg cattacgccg
    39121 agtcgtccgg gtgccggcgc agcgagttgc tgggctactt tggcgagccc gccgccgaag
    39181 ccaactgcgg cgggtgcgat aattgcctgt cgccccgggc gacctatgat gggacgctgt
    39241 cggcgcagaa gtttctttcg tgcctttacc gcatccggca gcgcagcgga tttgagtttg
    39301 gcatgaacca aatcgttgag gtgctgacgg gcgcggagac cgagagcatc cgccggtggg
    39361 gccacgaaca actctcgacc tatggcatcg gacaggagca cagccgggcg gagtggaagg
    39421 ccattgggcg cgagctgatc cgccagggcc tggtgcggca ggtgtcccgc gatcacttca
    39481 gcgtgctggg gttgacggag gatggcttgg cggtgctcaa ggagcgcaag ccggtccggc
    39541 tgacccgccc ggtcagcgcg cccgagccca aagccccgcg ggcaggggag attgcgtgcg
    39601 atgagctgtt gttcgagcgg ctggtgcggc tgcgcaagca actggcagat gagcgcgcgc
    39661 tgccgccgca tatccttttc tcggatgtga cgctgcggca ggtggcgcgc gactacccgg
    39721 ccagcgagcg ggcactggcg cgcatcagtg gcatgagcgc gcggaagctg caggagtttg
    39781 ggcgagtgct gctggcggtg gtcgccggtc atttggaaac gcaaccgcgc cagatttttg
    39841 cggagacctc ctttgaagac gacgttccaa gggaggcgcg gtcaacgcgc cgggcgtcct
    39901 ccgatgctgc gccgtatgac aaggaactgt tcgaacggct gcggatggtg cgccggagaa
    39961 tggccgagcg ccgcggtttg cccgcctaca tgatcctgca cgactcggcc ctgcgcgaaa
    40021 tggcgcggcg gtatccgcgg agcattgaag aattgactga gattgctcgt gtagggtccc
    40081 ggcgggcgag ccagttcggc gaggaattcc tgaaagtaat cgccgaacat gagtgcgcca
    40141 gcccacaagc ctcggattaa ccgattggcg ccggaagccg tcgccgttcc ggcactggcc
    40201 cggtctggaa ttgcccgggg atgcccataa aataagcgcc cgccaggtga tttccgcggc
    40261 gggcgcgcaa gccggaatct atgccggctg attactttct gccgccggca acttcctcgc
    40321 gatgcacttt atgcggcagg acgaggttgc cattggtgcc ggcccggtct tcctccggaa
    40381 tccgcatgag gtagaacgag cgatagacga agatgagcgc caccaggaag aagaacgcgc
    40441 caataccggt cttgaggttt tggttgggca tggtgaccgc gatttccacc gccaacaggc
    40501 cgaagagggt ggtgaacttg atgaccgggt tcatcgccac ggaggaggtg tccttgaagg
    40561 gatcgcccac ggtgtcgccc acaacggtgg cggcatgcag atcggtgccc ttctggcgga
    40621 ggtccacctc gacaatcttc ttggcgttat cccaggcgcc gccggcgttg gccatgaaga
    40681 tggcctggaa caggccaaag aacgcgatgg cgatcaggta accaataaag aagtagggat
    40741 taaagaacgg cagggccagc gccaggcaga agattacgat gaagatgttc cacatgcctt
    40801 tctgcgcata gacggtgcag atgcggacga cttccttgct gtccttctcg gaggcggtgg
    40861 aggcatcgag cttcatgtgc tccttgatgt aaaccaccgc gcggtaggcc cccgtgacga
    40921 cggcctggca ggaggcgccg gtaaaccagt agatcaccgc gccgcccatg atgaggccca
    40981 aaatgatctc gggctgcacg atgctcagcg cttccaccac cggcttgaag acgccggagg
    41041 tggggttggc ctcgaacatg tgctgcaaga ggatgatgat gccgaagacc atcgtcgtcg
    41101 cgccgacgac cgcggtgccg atgagcacgg gcttggcggt ggccttgaag gtgttgccgg
    41161 cgccgtcgcc cttttcgagc tgatacttgg cgttctcgaa gtccggctca aaaccgaagc
    41221 ccttcttgat ctcctccttg atgcctttgc ggctttcaat ctggctcaac tcatacacgg
    41281 attgggcgtt gtcggtcacc ggcccgtagc tgtccaccgc gatggtcacc gggcccatgc
    41341 ccagaaagcc gaaggccacc aggccgaacg caaaaatagg cgcggcgaag gcgaattgct
    41401 tgggcatgat ggcaagcagc gcttcattct gggagaaata ataagaaacg aacatcagca
    41461 gcaggatgca caaccccatc cagaaggcgg agaaattgcc cgccacgaag cccgagagaa
    41521 tattgagcga cgcgccgccg tgcttcgagc agttcgtcac ctcccgcacg tgccgggagc
    41581 cggtgctgac aaagattttg gtaaactcag gaatcagcgc gcccgcgacc gtgccgcaac
    41641 tgatgatgat cgaaagcacc caccagagat cgggcagagc ccggcccgcg gcgtccgtga
    41701 agtcgcccag gagcagtttg ctggcgatga aggtaatggc aatcgagacc gccgaggtga
    41761 tccagaccag gtgcgtgagc ggcgcctcga agtcaaagtc tttctggtgg ccgtatttgg
    41821 ccttgctgaa ggcctggttc cccaggtacg acgccaggga ggttacgatc atgagcgcgc
    41881 gcatagcgaa gatccaaatg atgagagtgg ctacaatccc gggagtttcc gccagcgcca
    41941 gcgccaggaa ggcgatcagc gccacgcccg tcacgccata ggtctcgaaa ccatccgccg
    42001 tcggcccgac cgagtcgccg gcgttgtcgc cggtgcagtc ggcaatcacc ccggggtttt
    42061 tgggatcgtc ctcgggtagc ttgaagacga tcttcatgag gtcggagccg atgtcggcga
    42121 tcttggtgaa gatacccccg cagatgcgca gcaccgaggc cccgagcgat tcgccgatgg
    42181 caaagccgat gaaacacggg ccaacgagat cgcggggcag gaagaccagg atgcaaatca
    42241 tgaagaacaa ctcgaccgcc accagcagca ggccgacgct catgccggag cgcagcggga
    42301 tgctcagggt ggcaaaggcg ttgccccgga gggcggaaaa ggcggcgcgg gaattggcca
    42361 cggtattgat gcgaatgccg aaccaggcga cgccgtagga ccccagaata cccaggatcg
    42421 aggccaacag aatgacgatg acattgacga ggttcatctt ggagagcacg ccaaagtagt
    42481 agatcatgca ggcggcgatc agcgcccaga ggatggccag gaacttgcct tgggtgaaca
    42541 ggtaggtctt gcaggtctcc caaatcatgt gggatacgtc gcgcatggaa tcatgcaccg
    42601 ggagggcttt ggtctggctg tattgcacca ggccgaacac ggccccaata acgcagatca
    42661 ccaagcccac atacatcaag gccgtgccgc tcaagccctg caaccccgca aatttcaccg
    42721 tatccagcgg tgggatttgg atgtcggcct cgctggcata aaccccggtt gacgccagca
    42781 gcgccatgaa taaaagaacg atcgccaaac aataataatt acgtttcata gatttaagcc
    42841 cgtgactatg gcagaaagcc accgggcaag ccactctttt ctttgagcga taggaagcgc
    42901 attggggcac taccctcaaa aatcgagatt gcaaggaaac gaatgagatt gaacccggaa
    42961 acgggttgcg actcgtcgga ggagtggggg gacgggctcg gcagatgctt cggaacgccc
    43021 ctggctgggc cgtgccgcga acggttgtgc agtgccgccc gagaagactg ataaaggccg
    43081 ttttcccaca agtgccgcgc ggacagtggg ttatcgttga actggagggg gagggatggc
    43141 tggtttacgg caggggcggg tgatttcggc tgaaacagtc actattggcc cgagagagtc
    43201 cccctaacgc gcgtcttggc agccctgaga accctataac cgcatgagct gccagtttgg
    43261 gaataccgct aaagcatggg atttggcctt ccgcagaaga aatataaata tatttgaaca
    43321 aattggagcc gcgtgttatc ttatcttcag gacgacagaa gatttcgctg ttcgtttagg
    43381 gttagcatag aggttttgct tgtaatgcgg ttcgaacagc gaccgaatag ttggccgcat
    43441 tacccttctg agcgtctggg tgaaggcgta taaacctc
//
LOCUS       DAOPQG010000012        43424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1129_length_43424_cov_15.574950, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000012 DAOPQG010000000
VERSION     DAOPQG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 43424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43424
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_43424_cov_15.574950"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PKI07_02380"
     CDS             <1..733
                     /locus_tag="PKI07_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HOC49928.1"
                     /translation="LRQQIPIRGSLWEEGAVGWLEVDTVALCGGSVAGEFVWVVDGVE
                     YVTTWVELRAIWGRGQINTLGALQDIEASLPFALLGLDSDNGGEFLNHHVLHWLQKRP
                     RPVFMTRSRPYKKDDNAHVEQKNWTHVRQCFGYERYDKAELIEPMNALLKGPYRQLRN
                     YFHASLKLERTQRQDGKVRRIYGAAQTPLARVLACPQVSPVTKAQLRQDKERLNPFAL
                     KQSVEQSLKQISALRQAAVPNARAA"
     gene            1006..1344
                     /locus_tag="PKI07_02385"
     CDS             1006..1344
                     /locus_tag="PKI07_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49929.1"
                     /translation="MPSVPAFDVITMLAVLEHFPQAGYASLRSGCSRFLKPGGHLIIT
                     VPSRQVDFILKVLLALGLIDGMSVEEHHGYDVRETANIFPPPEFKLVKRQRFQLGLNN
                     LFVFERQGEA"
     gene            complement(1428..2477)
                     /locus_tag="PKI07_02390"
     CDS             complement(1428..2477)
                     /locus_tag="PKI07_02390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOC49930.1"
                     /translation="MSPRLARRRGAWLLLLAALGGGPVLAQPSDGAPQLVSVNCIWNA
                     APHNAFTDLARHAGKWFCTFREAPAHVGGQGKIRVLTSADGRQWDSAAMLSEEGIDLR
                     DPKFSLAPDGRLMLVLGGSVYEGRVFKERQPRVAFSPDGRTWTAPRRVLEKNDWLWRV
                     TWHQNRAYGIAYNIAGGLLPTNEWRITLVASPDGVDWRPVAKLAVPGRPNEATIRFLA
                     NDDCIALVRREGLAPDQDKDAWIGQSAPPYTDWRWHSAGRQIGGPNFLVLPDGTMIAG
                     GRLYQSKPATDKTFIGRMNGESVRAELILPSGGDCSYPGLVWHDGLLWTSYYSSHEGQ
                     TSIYLAKVRLPGQSR"
     gene            complement(2479..3072)
                     /locus_tag="PKI07_02395"
     CDS             complement(2479..3072)
                     /locus_tag="PKI07_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOC49931.1"
                     /translation="MRRICIYCGSSPGARPEYAAAARHCGATLAARGVTVVYGGGNVG
                     LMGILADAALAAGGEVIGVIPHRMMARELGHERVTQLITVDSMHARKQKMAELADAFI
                     ALPGGIGTLEELFEVFTWLQLDLHHKPVGLLNVAGFYDRLVDFLAHVSGQRFLRPEHF
                     ETLIVEATMERLLDRFARFNHPPTGKWADKKPGAGGG"
     gene            complement(3212..4477)
                     /locus_tag="PKI07_02400"
     CDS             complement(3212..4477)
                     /locus_tag="PKI07_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49932.1"
                     /translation="MKTVSSLRMVSRNVKALAEAALQESGGLLRLAPNWVPRSFLQPG
                     LRIKLHPDDTYAYGAHRGGIDERWFASTTEAANEGRVPDEGLSYCVAGRQRFTLRQAV
                     AECGATLVGGAIWKKYGRWPVYSKFFDNMGPIPLHMHQNARQARLVGQEGKPESYYFP
                     PQQNNVGNNFPYTFFGLEPGTTKAQVRKCLEDWNKGDNGILDLSKAYRLKPGTGWLVG
                     PCILHAPGSLCTYEPQWGSDVFGMYQSMVEGRYVPWSLLVKDMPRNKHKDLDFIVDQL
                     DWEANVDPKFKDHHYLEPVPVADTRKEGYVDRWIVYGKIDGQQLFSAKELTVEAGVRC
                     TIKDRGAYGLICVQGVGKINGQPLNSPKLIRFGELTEDEYFCTEDGARAGVIFENTSE
                     TEPLVCLRYFGPEVNPDAPALGAYRKNKF"
     gene            complement(4607..6541)
                     /locus_tag="PKI07_02405"
     CDS             complement(4607..6541)
                     /locus_tag="PKI07_02405"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOC49933.1"
                     /translation="MTAIPNLSFARSAAAILPVPRRARWGLLLALGVLGWAPASAAAA
                     AAAEANPEAGLRILDPRGESTTTTRAVAHVLGRIAPGSKVWVEGQAAEVFATGIFARD
                     NVPLQLGENQLRVVATTPDGQKHEQVVRVKREPEAAAAPEPKERRLAIDAESLEPAQN
                     CILSRGDVLELSFRGTPGQEAEYCLSAGAWQPMHETRDAATGQPTGFYRAAFVAGATA
                     DTTNAPVRFRLRARPNEADGIRVTGEPVVEVSGRATVDLWDETRLRLVRVTEEGAGLS
                     FGLHEVRLGGPYLAELPAGTLLRVTGMREGNYHVRLSPGQDGWIARRAVEWAPPGTPP
                     PHLSFTSLSVGGNEAADLVAIPYSAPVPFAVTPARSAAGRAALEIDFYGAHHAATWIS
                     HRPAARVIREVTVQQVAAGHLRVSVELNGAQLWGYQWGVTNDAVMLRVRRPPKLAAAP
                     GSPLRGLVLALEPGHGGSDSGARGVSGSQEKNINRWAVEELARQLEAAGARTVMVRPQ
                     DEALSLGARVRRAVAADADLFISVHANAAGHARGYLSVSGTSTYYKWPFCRDLSAAIH
                     ARLLEIPRLNDFGNVGNFNYYPLRANTWMPTVLVEQAFMTHPGDEAKMLDPEFRREMM
                     RAVLLGAEDWLKRVRAEARD"
     gene            complement(6671..9667)
                     /locus_tag="PKI07_02410"
     CDS             complement(6671..9667)
                     /locus_tag="PKI07_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC49934.1"
                     /translation="MNGPRSWNRPLAGLIAGALLLGGGGIVWQCRHDPRIPFLPGAGP
                     AEWIVYPKPPDTTPHHAVPLWAEFRRAFTLPAQPAAATLAVRVFRQGAVRVNGQPVDG
                     LALHGADWKSRRAADVARFLKAGENEISVTVSNAHGLPALWLALEGAGPALYSDAAWQ
                     VSLVDAAWEAAVPASRPPAIRAGNPLFDRERTADSLRRAWPQGLLLLALAAALVAGHR
                     VLRARGSRFPPAPAVALGLILLAWIALLGNNLPQLAALFGFDRDGHQQYIDFILQQRA
                     LPLADDGWQMYQPPLYYLAAAALIGPFGWTASADAAILTLRAFSTLVGVAHLLLIYLC
                     LRQLFPQRPRPQVAGLLLAGFLPMSLCLAHHITNEGLAALFVTAALYGVLRPPPAGTR
                     GAGLAAAAGAGLGLALLTKFSAVLAAPLLLAALAWRRGAAGGARAAGSAAGLFLVSLL
                     AVCGWHFARVWHRFGTPLIGNWDPRLPFAWWQDPGAHTAAWYQRCGEALTCPLFSGIH
                     GFADGLYATLWGDGLCSGSALMNFRPPWNYDLMNIGSLLALLTTALIAAGTLVALVRF
                     LRRPTPEWFLMLGLLFAFAAGIGWMSLRVASYAQVKAFYALPALLPLCAVAAVGWDFA
                     ARRWPALRPWLRGGVLAWALTVYTAFWIRSGNAFTHTVRGVGLADDGRYDEAAAAFSR
                     ALQLEATSLPARVGLAEACRRRGRYADAQRETALALQQHPRAAAAHTQAALLLSLDRR
                     YPEAVEHLRQALAEAPDHPTAGQQLAACLAFMGQPRQVIAACRQGLRVDPFNPTLHHL
                     LAGAAAETGDLPGAVAHLHLALALKPQWPEARRLLALALTSLGRPDEAAVQYERMLQE
                     TPADPQLLYLYAGVLAAQGNPRAAVDRYRQALALQPDMVEALNDLAWIRAASSDAALR
                     EGAEAVRLAERACELSQRREPVLLGTLAAAYAEAARFDDAIRTAEQARALATAAGQHN
                     IAERNAQLLELYRAGRPYHEPAPPPR"
     gene            complement(9710..12226)
                     /locus_tag="PKI07_02415"
     CDS             complement(9710..12226)
                     /locus_tag="PKI07_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC49935.1"
                     /translation="MPDMAEGRKFEWTSAGAPRRTLLLCLLLALAIAAVYWPVLKFGF
                     INYDDPDYVSGNPRVQSGLTLENLAWAGQSFHASNWHPLTWLSHMLDCQLYGLKPWGH
                     HLTSVLLHLANTLLLFGLWQRLTGAVWRSALVAALFALHPLHVESVAWIAERKDVLSA
                     FFFLLTLWAYARYAGRREPADEENRKPGARPSAPAREAHDPGGGPLPPSSPSARGEPA
                     PPAEAGAVPGGKARASAETARRSPAAPRGAGWFYLLALLCFALGLMSKPMLVTLPCVL
                     LLLDWWPLERLALASGQRSAAPLRRVLLEKVPFFALSALSCLVTFQAQRASGAVMSLA
                     HNPFELRLANALVAYARYLGKTFWPTDLAVFYPYAGFRLDSWAAAGAGLLLAAVTAGI
                     WRARPPRRALQVGWLWFLGMLVPVIGLVQVGKQAMADRYTYLPHIGLFLLVVWGAAAV
                     IARRPWPRGIPAAAAALVLGACAVLTARQVAHWRDTATLFQHAARVTPRNFVAYAAIA
                     NEFAIQNQPTEAIAWCQKALAISPDYPEAHNTLGNVYASLARYDEAIAHYRAAAEGDR
                     LYADPLNGLGSALVKQGKFAEAEAPCREALRLAPLHLPALYSLATALHGQGKLEEAAA
                     CYRRLLALDPKLFTPRRLLGNVLIAQGRPEEAIGELQRALAIQPGDSEARTVLGMLLA
                     DRGQADDAAAQFREATRLQATNALAHYQLALLHQGRKETRAAVERLRLALKAQPDWPE
                     SLNNLAWILAASADATVRNGAEAVTLAERACQITAYQEPLLIGTLAAAYAEAGRFPEA
                     INAAEKARALAQSAGLTAVAQRNEELLKLYRAGQAYHEPE"
     gene            12818..13255
                     /locus_tag="PKI07_02420"
     CDS             12818..13255
                     /locus_tag="PKI07_02420"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOC49936.1"
                     /translation="MTGLTDVLQLRVRYSETDQMGTFYNSRALEWFECGRTELMRRRL
                     GMSYTVLEARGVFLPLVEAHLEFLGGARYDDLLEVVSTVKLSGRARLRFEVQIHQSES
                     RRPVVRGYTVHAFTDRQARPLRPPPWFLEMLAGAMPAPGAPPS"
     gene            13320..14651
                     /locus_tag="PKI07_02425"
     CDS             13320..14651
                     /locus_tag="PKI07_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOC49937.1"
                     /translation="MSKHAKNSERNFHPLSAPDFLPLPRLRDLQFQRLKATVQRAYDR
                     VALFRQRMEERDLTPRDVQSLDDLAKLPFTGKNDLRDTYPFGLFASPMREIVRLHASS
                     GTTGKPIVVAYTQEDVQVWTNVMLRSLAACGLHEGDIIQNAYGYGLFTGGLGAHYGAE
                     ALGATVIPISGGNTPRQLMVMKDFGVTAICCTPSYFLHLIDQAPEAGIQFKDLPLRAG
                     IFGAEPWTEAMRRRIEADSGIKAYDIYGLSEIVGPGVAMECECQAGPHIFEDYFYPEI
                     LHLKTGKPCADGEEGELVLTTLGKQAMPMIRYRTRDITALASETCECGRTLRRIKRIG
                     RRADDMFIIRGINVYPSQVETALLKVEGALPHYQIVLTREEDLDVMEVQVEVTPEVFN
                     DTVGALEALRLKLTKSLETIVGVRAKVRLVQPRTIQRSEGKAKRVIDQRKM"
     gene            14670..15110
                     /locus_tag="PKI07_02430"
     CDS             14670..15110
                     /locus_tag="PKI07_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOC49938.1"
                     /translation="MKVKQLSLFLENKPGALSRPIKLLAKARLNILTLSIADTQQFGI
                     LRFVVRDWEKAKRLLEKEQFVVKVSDLVAIEVANEPGGLAGILLALERARVNVEYMYG
                     FTLRAEGKGVLAFRFDDPDTAIASLLGAGINPIRRVDLFNRLTG"
     gene            15340..18375
                     /locus_tag="PKI07_02435"
     CDS             15340..18375
                     /locus_tag="PKI07_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOC49939.1"
                     /translation="MNTMKPILTRPTLRGWLLALAALALSSLIVHAVPYATEVVKNGN
                     TVSYILNQNAASVEVLRNGGNALYPGTNAGAYNFDMTGYTTFQIKVIGNDAPGWVQYV
                     PDNKDVTGFEYPYGVSVNKNPQSPNFGKVYVSNSRVGTTGLGRSCTTAIYMLHADGSD
                     AGFSDGGVYWTPGGSGPYKSCIGPDDHLYVADLSNDLAYEFSDDMSTATLLIDDSNRT
                     PNQYVHSIYVEGTQAAGNRKLYLVNGNINDTARKGLIQYDLGANAAATAWDTGTQIIG
                     PTYWGIYYSYDVARDSNGDWYMPSYRANPNQRPAITKFDGSQTPPLDNYVLWETDKSL
                     YLYSFGIDICELNGLAAYGHANNGNVYFFKMTDGSFVENFDAGGAIRDLAFDAAGNMV
                     TVDNAAEWARFWSPGGYTVATTTFDGSQTTFQVTRPPAQVSVTASQATVPEAGPAVNF
                     IISRVGSVAADLTVYYTLSGTADNGVDYTTLSGSAVIPANANSVNVALAPIDDAVAEL
                     SETVILTVMANSNYSVALPTSATISILDNETPEISFAAAPPRKLLESYAPSRVTHQVV
                     RKGLLTPELTVNLSYSGTATRGVDYNAPAAVTVPAGAATANLVLTPINDQDYEGDETA
                     TANIAGGTGYTIGTPSSIDATVVDDDPPPGTVLFSDNFDTDSSALWEVNLYDPSDASV
                     NFAWDYSTVGIPAAPAGGGTKGLRMRCGNTQLILNGLSVSPKNKSFTGDYRLKFDMWI
                     NYNGPMYDGGPGSTQNFDAGVGTSGQIPVWFWAPSPDDSVWFTVAGDGASGFTVGDYN
                     AMIGATLLNDDSGVYAAGTGAPNSGIRDASNAYYALWGGQAAPAAQLAMYPNQTGNAN
                     IGNAGMAWHTVVITKEGDVVTWVMDGITIATVTNTALPFGPNVFVGYQDLWASGSLSD
                     VPEMSFGLVDNLKVMTLAAPADIVITGIQLINGQADVQIDFTAGAGDAPADFTLQATA
                     NIASAPADVAANITSTGPGQFKAVRAVAGTAQFYRLRRN"
     gene            complement(18474..18917)
                     /locus_tag="PKI07_02440"
     CDS             complement(18474..18917)
                     /locus_tag="PKI07_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOC49940.1"
                     /translation="MNPPASSPTAHPDGALDRIRRFFLGDKFAAHASIELLAVSPGHA
                     RARMTLGPHHLNGYGTVQGGAIFTLADFAFAAASNSHGTVAVAANVSITFMKAGRAGT
                     LWAEARELSRNFKLGSYTVDVKDDAGDLVAVFQGLAYRKSEKIPD"
     gene            complement(18919..19416)
                     /locus_tag="PKI07_02445"
     CDS             complement(18919..19416)
                     /locus_tag="PKI07_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOC49941.1"
                     /translation="MTKVTNVVVAGLGGQGVLRGTDILADVALRAGYDVKKSEIKGMS
                     QRGGSVTGDVRFGGQVFSPMVAAGEADFLLVLEPTQVAPQQHMLRPGGVLITPDAVRA
                     DQLPSRKTLNVALLGALSAHLPMTEEQWLAALRAGFAENFFAGNRQAFLTGREAQKQA
                     SPNLK"
     gene            complement(19444..21066)
                     /locus_tag="PKI07_02450"
     CDS             complement(19444..21066)
                     /locus_tag="PKI07_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC49942.1"
                     /translation="MNESNRLLLSGDEAIALAARHAGVALGAGYPGTPSTEILEHFHA
                     LGGHAQWSPNEKVALEVALGAAFGGARALCTMKHVGLNVAADPLFTAAYTGVTGALIV
                     VSADDPGMASSQNEQDNRHYAVAAGLPMLEPSDSQEAYDFLFAAVEISERWKLPVLFR
                     VTTRVCHSYTVVRPRAQTEAPRPANYEKDVQGRVMIPAYARPAHRRLRQKLAELREWN
                     EASPLNRLVDGAPALGIITSGISFMHVREAAPEARVLKLGFTHPLPLKRIAQFARGVE
                     RCLVVEEGDPFLVEAIRAAGIPVEGKADRFRFGELDVPRVRRILQRDESPEPSKPPGK
                     PPQLCDGCQYRIVFEALRRKDCIVAGDIGCYTLGVLSPFEAIDTCVCMGASVGVGLGL
                     RHVLPPEQARRVVSVIGDSTFVHSGINGLVEMVYNPPPLGHVLIILDNSTTAMTGHQE
                     HPGTGRTLGHEPTGQVVLEDLARALGIRRVHVVEPRLGSGDFERVLDECLASGELAVI
                     IARRPCILIAKQLKEYEARRCECAAAASPASP"
     gene            21193..22485
                     /locus_tag="PKI07_02455"
     CDS             21193..22485
                     /locus_tag="PKI07_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOC49943.1"
                     /translation="MFWDQASETLARPTLEHLQIKLLQETLHRVRERVPFYRQRLAEL
                     NLQPADIRTLAEVRRLPFTTSDDLRAIYPDGLLAVDRAEAVRLHTSSGTTGKPKAIFF
                     SRADVDNAAELIARSLVSAGITAADVFQNMMSYGLFTGGLVMHYGAEKVGCLVIPAGP
                     GTSERQLMLMQDFRTTAVHILPSYALYFASYLEQKGLRPRQDLTVRKAFVGAEPHTEE
                     TRRRIEQIFGCDVYNSYGLTEMNGPGVACECEYKAGMHLWEDNFLLEIINPVTNEPLP
                     DGQTGELVLTTLRREAMPILRYRTRDITSVVPEPCRCGRTHRRLARFVGRADDMLIIR
                     GVNIFPQQVERVLMSFPQVGRNYLIIVEGLDDLTIKVELTPAAFDGQLEHLEALQQQI
                     IEKLRAEIWIRPKVDLVPAGSLPVGEGKAKRVVDKRSL"
     gene            22513..22941
                     /locus_tag="PKI07_02460"
     CDS             22513..22941
                     /locus_tag="PKI07_02460"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOC49944.1"
                     /translation="MRVVNLLAVFVENKPGQTARIAGLLAEAGINLSWLTIANSGSFG
                     VMRFLVDQRDTAVRALKDKGVMVSLVEALAVEVPDQPGSLQAVADLLGRNQINLDNCS
                     GFVVRDRAILIIEVHQIAQAGAILEQQGFRLLGQEEMLRL"
     gene            complement(22989..23993)
                     /locus_tag="PKI07_02465"
     CDS             complement(22989..23993)
                     /locus_tag="PKI07_02465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOC49945.1"
                     /translation="MNRNPHVAVVGATGAVGIEMIKTLEKRNFPAGKLTLLASARSVG
                     KKLKYRGQELAVAELKKDSFGGIDIALFSAGGSISKEYAPAAAQAGCVVIDNSSAFRM
                     DDRVPLVIPEINAADLKWHKGIIANPNCTTAITLIALYPLHAAFQCRRIFASSYQAVS
                     GTGAKAITELERQVEQVVQRQPVTREVYPHQIAFNVLPHVDSFLPNGYTREEMKMENE
                     GRKIMHHPTFRASVTCVRVPVYRAHSIAISAEFERPVTVEAALAVLRKAPGLDVIDNP
                     AQSEYPLPLYLSGKYNCAVGRLRQDCALDNGLCFWVSGDQLLKGAALNAVQIAEELIK
                     "
     gene            complement(24388..25461)
                     /locus_tag="PKI07_02470"
     CDS             complement(24388..25461)
                     /locus_tag="PKI07_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HOC49946.1"
                     /translation="MNHGLRLLEPIRLGGLDLPNRVFMSPLTRCRASAGRVPNLLMRD
                     YYVQRASAGLILSEATAISPMAVGYPDTPGIWSPEQVQGWKTITEAVHAAGGRIFLQL
                     WHVGRISDPSYLGGAQPVAPSAIPARGHLSLLRPKRPFATPRALATDELPGIVDAYRQ
                     AAQNAQRAGFDGVEIHGANGYLLDQFLQDSTNRRTDDYGGPIENRARLMLEVTDAIIA
                     VWGADRVAMHLAPRCDSHDMGDSDPAATFGYVARELGKRRIAFICARESIGPNRLGPA
                     LKKQFGGLYVANEAHTRDTAEQLLQAGEADAVAFGKLFIANPDLPRRFALNAPLNTPV
                     PETFYASGPGGYTDYPALPPTAA"
     gene            25601..26845
                     /locus_tag="PKI07_02475"
     CDS             25601..26845
                     /locus_tag="PKI07_02475"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOC49947.1"
                     /translation="MKIVLAYSGGLDTSVILSWLKETYQAEIIAYCANIGQEEELKGL
                     AAKARRTGGSKCCIADLQEEFARDFIFPMIQAGAVYESQYFLGTSIARPLIAKGMVEV
                     ARREKAQAIAHGATGKGNDQVRFELTAAALAPELEVIAPWRDARFRAEFPGRAEMIAY
                     CAKKRIPVQASAKKPYSMDRNLLHISFEAGILEDPWLDAFAPGNKDMFVLSVAPEDAP
                     DKAEYVTLDFQQGNCVAVNGRALSPLGVMRTLNRLGGRHGVGRVDLVENRYVGMKSRG
                     VYETPGGAILQFAHRQMETLTMDREVMHLRDSLIPRYAELVYYGYWFSPERLALQAFV
                     AESQKNVTGTVRVKLYKGNILCAGRKSPVSLYHPAIATMEADPTQAYNQDDATGFIRL
                     NALRLKVAARVQRKPRPAARRG"
     gene            complement(26842..27855)
                     /locus_tag="PKI07_02480"
     CDS             complement(26842..27855)
                     /locus_tag="PKI07_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC49948.1"
                     /translation="MNCFVTGASGFIGANLVHELAARQHRVKALLRPGCDLRGLAGAE
                     FERLEGDLSDAARLTAALRGCEWCFHVAASYHLWLRDYRPMYAANVEGTRTVIQAAAA
                     AGCQRIVYTSTVGCLALPEPGAGPTPPVDETAVAGESQMVNHYKKSKWQAEQVALRLA
                     RDGCPVVIVNPSAPVGPRDVKPTPTGQVIVDFLNRQMPAYLDTGLNYVHVRDVAAGHI
                     LAAQRGRIGERYILGHADGNWTMRQAFAVLEEITGIRAPRLRIPHAVALGAAGISEAL
                     ARLTHRPPKAPLAGVRMARYKMFFNPAKAIRELGLPQTPPRQALADAVAWFRQNGYVT
                     RGG"
     gene            complement(27983..28384)
                     /locus_tag="PKI07_02485"
     CDS             complement(27983..28384)
                     /locus_tag="PKI07_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOC49949.1"
                     /translation="MKKLSLIAAAALGALIACNAAPAQGGAKNPAWKGGKREAPNLEQ
                     RLERMTTQLKLTDAQKPKVKAVLEETAKKIKELKPEERPAKIKTLREEETKKLKAILT
                     DEQFKKREEMMQRAREKRGPIGDKKARKARA"
     gene            28668..29312
                     /locus_tag="PKI07_02490"
     CDS             28668..29312
                     /locus_tag="PKI07_02490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC49950.1"
                     /translation="MKIATTWCAFSLALGLVAAQGQGTNNPTKAETKPAEQAGTQQKK
                     DPVNTKEVAVIKTTEGEMVIEFWPEVAPRTVENFKTLAKKGFYDGTCFHRVIKGFMIQ
                     GGDPLTKDPALEARWGTGDPGYKIKAEFNERHHDRGVISMARSQDPDSAGSQFFICHG
                     NPRFLDRQYTAFGKLIKGDDVLEKIATTATHPPDRPNQRMGIESIKIVAADSVK"
     gene            29434..29509
                     /locus_tag="PKI07_02495"
     tRNA            29434..29509
                     /locus_tag="PKI07_02495"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29467..29469,aa:Lys,seq:ttt)
     gene            29742..31652
                     /locus_tag="PKI07_02500"
     CDS             29742..31652
                     /locus_tag="PKI07_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49951.1"
                     /translation="MMLAFLKFPPCSEPDEERAYRADPSQTAVLFYAQQHGLQFGKDI
                     AYTYGPLGFLLMDYFYPWAAWLRLVVEVGLDFMVALGICLVAWRLHWGWRCLLLGGCA
                     WGAANIFAGDIHPRCDLVINAGLLCWGLLGFVESGRRLAWVTAAFGALAVFCALAKVS
                     YLFLAAGLVPLLSGDLWLRGRRRLALGLGAGCAGGFLLGWKLAGQSLAYLAPFLANAV
                     AMVRAYNAALGFEPLLWARNLGWVLAPLFLALLWLRARSAFDGPAGRVWGRRGFLLAW
                     LLLLTFAAWKHGFVRAYAGYLTLCFFYLALLAVALEVFVAGKPGRGRGDALLSGVCWF
                     LPLAGAQALSFPAFPGSVAVAVRAAGANLPALLRPGEYLRRENAAFAENRRRAQLPRF
                     RELIGSARVDVFGYRQAFAILNDLNYRPRPNFQSYGACNADMMRLNERFYLSRAAPEY
                     VLFEFLLLDRKWPSLEDGYVLRQLLMNYEPVATEQNFILFRARAADAPRLTLLREGTA
                     RPGDAIDLSVGGGANLWLEIDLRPSWSGRVRQLLARPPPVRLAAWRELRRGLLLRKRA
                     PASMLAAGFLASPLLQRNPDLLELYAAHAATRPRAYSVELLPGDERFWQVPFRFRIYR
                     IENPLGRRTPNP"
     gene            31788..32879
                     /locus_tag="PKI07_02505"
     CDS             31788..32879
                     /locus_tag="PKI07_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HOC49952.1"
                     /translation="MLVIGTFQLPAQQSEADRKWLAEVRAKAEKGDAHSQCELGRAFA
                     FGNRGVTQDEVEAVEWYRKAAEQNLVKAQFYLGICYSQGQGVAKDEAEAYKWFLLAAG
                     QGDKGAIKQVDKLKRSMTRRQIAEGQKRARDFKPREAPSSGSDNSIPRILETRPEGSG
                     TGFFITEDGYLITNEHVAGNGALVRVVTQDGILSARVVRVDSANDLALLKAEGRFAPL
                     PLVSSRDVKLGRTVATVGFPNIGLQGFAPKLAKGEIAALSGAQDDPRYFQISAPVQPG
                     NSGGALVDERGNVVGVVTAKLSAKTTLAASGALPENVNYAVKSSFLLSFLESVPEVAA
                     RLREPNLKDRKFEDVVKAAEQAAVLVLVY"
     gene            33125..34600
                     /locus_tag="PKI07_02510"
     CDS             33125..34600
                     /locus_tag="PKI07_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC49953.1"
                     /translation="MRKGAAVIEGFLAKFTVLKGASRELWITFAVKFLGVIAYKVTVL
                     TLALWLMADFGYGDADAGSLVAGWSILMTVVTLLVGSLTDAIGLRRTFFLGVWLSVAA
                     RAVMTFTTIKWLALAAGLFPLAVAEALGTPVLVAAVRKYSTTKQRSISYSLFYTIMNL
                     GFLLAAFLFDHLRQSLGEQGRLTLPLVHCQLTTYRTLFFASLGIQLAILPVIYFIRRD
                     VEVTDEGIRFQPAARGPVGLGFWRVCWQTVRDSARDTVKLFGGLLRQAGFYRLLAFLM
                     LISFLKLIFMQMDYVFPKFGIRELGEGAPVGRLVAINNILIVFLVPLIGALTQRFTAY
                     RMVIVGGLISAASVFIMALPTLWFQPLANGWVGHWIGHGYLRLTGEVHPYYVMITFFV
                     ILMSFGEAFYSPRVYEYAAAIAPRGQEASYAALSYIPFLLAKLIVGTVSFRLLARYCP
                     EVGVRNSGVMWLVIGIIAAVAPVGLILLRRFIRVQEAGREG"
     gene            complement(34656..35609)
                     /locus_tag="PKI07_02515"
     CDS             complement(34656..35609)
                     /locus_tag="PKI07_02515"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOC49954.1"
                     /translation="MLIKPDRLTRGDTIGIVAPASAPPDPKTIDRAAAALEHLGFKPL
                     LAPNVRRRHGFLAGSDRDRASDLMRMFAHRKVKAILCVRGGYGSARLLPLLDYHLIRA
                     NPKIFVGYSDITSLHCAFLTHARLVSFHGPMLNSDFVKPDLPSFTRQGFLRTLMQPSP
                     PGSICRGYRRKTVTILRRGIASGPLVGGNLSLLCAALATPGQPPVKNSILFLEDVEEA
                     PYRFDRMLTQLLNAGFLQQVAGIAIGVNADCKDPKAKGTREYRQTLEDVLRERLLPLK
                     VPLVMGLPFGHVSHNATLPLGVRATLDALRGDLVITEAAVS"
     gene            complement(35627..38167)
                     /locus_tag="PKI07_02520"
     CDS             complement(35627..38167)
                     /locus_tag="PKI07_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC49955.1"
                     /translation="MTDELNKGTARPDAARPKVPAEDRVAVLLVDDQVLVFEAIRRAL
                     AGQPNLDLHYCADAREALAMASRIKPCVILQDLVMPGADGLALVEQLRANPATRDTSI
                     IVLSTNEDPQVKGRAFALGANDYLVKLPDKIELAARIRHHAQACLNLSQRDAAYRALR
                     ESQQQLIASNAALISLNHQLEDATAAKSQFLANMSHEIRTPMNGIIGMTGLLLDTELT
                     DEQREYVEATRNSAEALLTIINDILDFSKIESGKLELEYRPFELRTCLEEVIKLLAPR
                     AAQKNLDLACLVDDAIPRILVSDVTRLRQILVNLIGNAVKFTQQGEVVVEVVPDEHGA
                     RSLPPGFAPDTEFLRHPNHWLLHFSVRDTGIGIPLDRQSRLFKSFQQVDAATTRHYGG
                     TGLGLAISKRLAELMGGRIWVESEAGQGATFHFTISTRAPAAATPPAWLNPQPQLAGR
                     RVLVIEDNPTNRRIISHRGQQWGMKVQTADNRREALALLAQGDLFDAVILDWELPGQE
                     GPALAEQIRKLPSGRCVPLLLLSAMRPSGEDAHPVREGMAVAIHKPIRPAQLLEALCH
                     ALKVQTLREKKPPPTPTLDPTLAHKLPLRVLLADDNPINQKVGLSVLSKLGYRADVVS
                     NGREVLETLGQKPYDLLFLDVQMPEMDGLEAARQICQRWPADKRPRIIAMTGNAFVGD
                     REKCLQAGMDDYISKPVRVGDLQSAIERWGPTRPKRSDTTLLARARVIPSEKLLDYAL
                     IAELRNLPATGSVGVFQELVDLFLQNAPQRIIQINQSVNDGPMLTFHAHALKGSALNL
                     GATRLAEISQKLEDLGRARNMHGVPALVQELDSVFTQTRAQLLSLRNQ"
     gene            complement(38316..40550)
                     /locus_tag="PKI07_02525"
     CDS             complement(38316..40550)
                     /locus_tag="PKI07_02525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOC49956.1"
                     /translation="MRELFRVEAENQVAILTRGLLELEQKPATSEHLAELMRAAHSLK
                     GAARIVGLEAAVQIAHALEDCFVLAQRGKLVLRQPEVDLLLRGTDELLQLARQAETPG
                     AGGESAPAAETQTLLRALADLAQAGDAAAAAPPPPAIPDTAPPAPEEMPPPASVPPGP
                     GVPERVVRLTAENLNRLLGLAGESLVEARWMRPFADSLQRLKRQQNELWQQLEELRRH
                     GDEAGLPARTRHQLAELAHKAAACQQLLADRLQELDLFDRRSAQLSQRLYLEVLRTRM
                     RPFSDGVRRFPRMVRDLAHSLGKKARLEIIGESVPVDRDLLERLESPLAHLLRNAVDH
                     GCETPEQRHRLGKPREGTIRLEARHSAGQLLITVADDGPGVPLELVREAARQRNLADP
                     AAAEKFTAPELLDFLCLPGFTLKETVTEISGRGVGLDVVQNMVRGVRGHLRISTQPGQ
                     GLQVQLQLPLTLSVLRALLVEVDGEPYAFPLNQITRALRLPRAAVETLEGRHHFHFAG
                     RQVALVTAHQVFDAEAPQPRDDDWAVVVLGNRNASYGVLVDRFLGERELVVQPLDARL
                     GKVNNISSAALLENGAPVLIVDVDDLLRSLGKLAAGQPLKLVPPQPPAPAGRRRKRIL
                     VVDDSLIVRELMRKLLASRGYLTDGATDGLDGWNAVRASQYDLVIADVDMPRLSGIEL
                     TSLIKQDPQLKSLPVMIISNQDRERDQSRGRITGADFYLTKSAYRDDALLQAVADLIG
                     APDA"
     gene            complement(40586..41278)
                     /locus_tag="PKI07_02530"
     CDS             complement(40586..41278)
                     /locus_tag="PKI07_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOC49957.1"
                     /translation="MKTPDSPASPPAVLDPRACWNEIGGQGNGTCPKLAEVIRCRNCP
                     VFSAAARQLLDRPLTPDYRRAWTEHFAQAQTPAAPASLAVLLFRLQAEWLALPAQVFQ
                     EVAERRPVHSLPHRRQGIVLGLVNVRGELLLCVSLARLLRRDEPPRPEARQAAYDRLL
                     VANWAGQRYAFPADEVRGIQKFQAAELTEPPATLAKSGLAYTRGLLPWQGRVVGLLDA
                     ERVFSSLNRSLA"
     gene            complement(41362..43176)
                     /locus_tag="PKI07_02535"
     CDS             complement(41362..43176)
                     /locus_tag="PKI07_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOC49958.1"
                     /translation="MANKASEPAAATGAPNTPSDFVRDIVIAHVAEKRYPRIHTRFPP
                     EPNGYLHIGHAKSICLNFGIAREFGGVCNLRMDDTNPTKEDVEYVDSIIEDVKWLIRG
                     WADDRLGLKPKGKFPVAQTVNGKNDFYLGPVTGASEVPLEPFYASDYFDQTYEYAVQL
                     IKKGVAYVDDLSAEDTDKYRGAPDRPGQESPFRNRSIEENLDLFVRMKNGEFPDGART
                     LRAKIDMTSPNVWMRDPVLYRIRHAEHHHAGAKWVIYPMYDFAHCLSDYLEGITHSIC
                     TLEFEVHRPLYDWILEQLDLPRPLPHQYEFARLSLGYTVMSKRKLMQLVNENLVSGWD
                     DPRMPTISGLRRRGVTPEALRDFAYNIGITKYNGITDVSVLEYCIREDLNRRALRRLA
                     VLRPLKVVITNYPEGQVEELEAVNNPEDPGAGKRKVPFSRELYIEREDFMEAPPPKYF
                     RLRPGGEVRLKYAYIIKCDEVIKDAAGQVVELRCTADLGSKTGGPTAARKVKGTVHWV
                     SAAHAMDAEVRLYDRLFTVPVPDASGDFKQHLNPHSLEVVTAKCEPGLKDARPELRYQ
                     FERLGYFTLDQDSTPDRRVFNRTIALKDTWAKEARKSA"
BASE COUNT         7249 a        13905 c        14730 g         7540 t
ORIGIN      
        1 gctgcgccag cagattccca tccggggcag cctttgggaa gagggagcgg tgggctggct
       61 ggaggtcgat acggtcgcct tgtgcggcgg cagcgtcgcc ggggagtttg tttgggtggt
      121 tgacggggtg gagtacgtga ccacctgggt ggaattgcgc gccatctggg gtcgaggcca
      181 gatcaacacg ctgggtgccc tccaggacat cgaagccagt ttgccctttg ccttgctggg
      241 gttggacagc gacaacggcg gggagttcct caatcaccac gtgctgcact ggctccaaaa
      301 gcggccacga ccggtgttca tgacccgcag ccgcccctac aagaaggacg acaacgcgca
      361 cgtggagcag aagaattgga cccatgtgcg gcagtgcttt gggtatgaac gctacgacaa
      421 ggccgagctc atcgagccga tgaacgcact gctcaagggg ccctaccgtc aactgcgcaa
      481 ctacttccac gcttcgctca aactggaacg aacccagcgc caggacggca aggtgcggcg
      541 catttacgga gcggcccaga ctcccttggc gcgggtgctg gcctgcccgc aggtcagtcc
      601 ggtcaccaaa gcgcaactgc ggcaggacaa ggaacgactc aacccctttg cgctcaagca
      661 atcggtggaa cagagcctca agcaaatctc ggccctgcgc caggcagccg tccccaacgc
      721 acgagcggcc taaccgctgg acacaaccgt gcagcagatt gtgaataact ctcttaacca
      781 gccctccccc ccagcccccc accacggcag tccctggttt tcgaccgcag gggggcgcga
      841 gctaaggctc gcagctcgcc cccctgctcg ttttagcttg cttcggaccc gcaaaaccac
      901 taacctaaac aatcaccgcc gggtaacacc ccttttggcg caacgatacc accttcaggt
      961 atcccctcgg gtaacagtta cttttggcgc acggagcccc aagacatgcc ttcggtgccg
     1021 gcttttgatg tgatcaccat gctggcggtg ttggagcatt ttcctcaagc cggctacgcc
     1081 agtttgcgtt cgggctgctc ccgattttta aagccgggcg gccacctgat catcacggtg
     1141 ccctcacgcc aggtggattt catcctcaaa gtattgctgg ccctgggctt gattgatgga
     1201 atgtcggtag aggaacatca tggctacgac gtgcgggaga cagcgaatat tttcccgcca
     1261 ccggagttta agctcgtgaa gcgccaacgc tttcaattgg gcttgaacaa cctgtttgtg
     1321 tttgagcgac agggggaagc gtaaaatggc ccgcgtgcgt gcgctcaagg tcagaccgct
     1381 ctcccgcatt ttcgcgcggg cttgcggcag gcattgaaac tgcggcctca gcgcgactgg
     1441 cccggcaggc gcactttggc caggtaaatc gaggtctggc cctcgtggct ggagtagtag
     1501 ctcgtccaga gcaagccgtc gtgccacacc aggccggggt agctgcaatc gccgccgctc
     1561 ggcagaatca gttccgcccg gaccgattcc ccattcatgc ggccgataaa ggttttgtcc
     1621 gtggcgggtt ttgattggta caggcggccg ccggcgatca tcgtgccgtc gggcaggacc
     1681 agaaaattgg gccctccgat ctgccggccg gccgaatgcc agcgccaatc ggtgtacggc
     1741 ggagcgcttt ggccgatcca ggcatcttta tcctgatccg gggccaggcc ctcgcggcgc
     1801 accagggcaa tacaatcgtc attagccaga aagcggatcg tggcttcgtt gggccggccc
     1861 ggcacggcga gtttcgccac cgggcgccag tccacgccat ccgggctggc caccagggta
     1921 atccgccatt cattcgtggg cagcaggccg ccggcgatgt tgtaggcgat gccgtaggcg
     1981 cggttttggt gccaggtgac gcgccagagc caatcgtttt tctcgagcac gcgccgcggc
     2041 gcggtccagg tgcggccatc cggagagaag gccacgcgcg gctggcgctc cttgaaaacg
     2101 cggccttcgt acaccgagcc gcccagcacc agcatcaggc ggccgtcagg cgcgagggaa
     2161 aacttggggt cgcgcaaatc aatgccttcc tcgcttagca tcgcggcgga atcccattgc
     2221 cggccatccg cggaggtcag cacgcggatt ttgccctgac cgcccacatg cgccggcgcc
     2281 tcgcgaaagg tgcagaacca ttttccggca tgccgcgcca ggtccgtgaa cgcattgtgc
     2341 ggcgcggcat tccagatgca gttcacggag accagttgcg gggcgccatc cgaaggctgg
     2401 gcgagcacgg gaccgccccc gagcgcggcc agcaaaagca gccacgcccc gcgccgccgg
     2461 gcaagacggg ggctcatgtc agccgccgcc cgcaccgggc tttttgtccg cccatttccc
     2521 ggtgggcggg tggttgaagc gggcaaaccg gtcgagcaaa cgctccatcg tggcctcgac
     2581 aatcagtgtc tcgaagtgct cgggccggag aaaacgctgg ccgcttacgt gggccaggaa
     2641 gtccaccagc cgatcataaa aacccgccac gttcagcagg ccaacgggct tgtgatgcaa
     2701 gtcaagctgg agccaggtaa acacctcgaa cagctcttcc agggtgccaa tcccgcccgg
     2761 cagcgcgata aaggcgtcgg ccagctcggc catcttctgc ttccgcgcgt gcatcgaatc
     2821 cacggtgatg agctgcgtca cccgctcatg ccccaattcg cgcgccatca tccggtgcgg
     2881 gatcacgcca atgacctcgc cgcccgccgc cagcgccgca tcggccagga tgcccatcag
     2941 cccgacattt ccgccgccat aaacaaccgt cactccccgc gccgccaggg tcgcgccgca
     3001 atgccgggct gcggcggcat attccggccg cgccccggga ctcgagccgc aatagataca
     3061 gattcgcctc atccgggagg gaaagattgc ccgtcaaaca cgcacccgtc cgcacatccg
     3121 gccccgcccc ccatctccgc cgcggcgcgg tccgggtccc ttcccatcgc cgtctccggg
     3181 gccgtttttt cgccgggcaa gcgggccgcg tttagaactt gttcttgcgg taagcgccca
     3241 aagccggggc gtcgggattc acttccgggc cgaagtagcg gaggcagacc agcggctcgg
     3301 tttcgctggt gttctcgaag atgacgcccg cccgggcgcc gtcctcggtg cagaaatatt
     3361 catcctcggt caactcgcca aagcggatga gcttgggact gttgagcggc tggccgttta
     3421 tcttgccgac gccctggacg cagatcaggc cgtaggcgcc ccggtctttg atcgtgcatc
     3481 gcacgccggc ctcgacggtg agttccttgg ccgagaaaag ctgctggccg tcaatcttgc
     3541 catacacgat ccagcggtcc acatagccct ctttgcgggt atcggccacc ggcacgggtt
     3601 cgaggtaatg gtggtctttg aacttcgggt ccacgttggc ctcccaatcg agctggtcca
     3661 cgatgaagtc caggtcctta tgcttgttcc ggggcatgtc tttgaccagg agcgaccacg
     3721 gcacgtagcg gccttcgacc atgctttggt acatgccgaa aacatcgctg ccccactgcg
     3781 gctcgtaggt gcaaagcgag ccgggagcgt gcaggatgca gggccccacc agccagcccg
     3841 tgccgggttt gagccggtag gccttggaga gatcaagaat gccgttgtcg cccttattcc
     3901 agtcttccag gcatttgcgc acctgggcct tggtggtgcc gggctccagg ccgaagaagg
     3961 tgtaggggaa attgttgccg acgttgttct gctgcggcgg gaaataatac gactcgggct
     4021 tgccctcctg gccgaccagc cgggcctgcc gggcgttctg gtgcatatgc agggggatgg
     4081 gccccatgtt atcaaagaac ttcgaataca ccggccagcg cccatacttc ttccagatgg
     4141 ccccgcccac gagcgtggcg ccgcattcgg ccaccgcctg gcgcagggtg aaccgctgcc
     4201 ggcccgcgac gcagtagctg agcccctcgt cgggcacgcg gccctcattg gcggcctcgg
     4261 tggtcgaggc aaaccagcgc tcgtcaatgc cgccgcgatg ggcgccatag gcgtaggtgt
     4321 cgtccgggtg cagcttgatg cgcaagccgg gctggaggaa ggagcgcgga acccaattcg
     4381 gcgcgaggcg cagcaggccg ccgctctctt gcagggcggc ttccgccagg gctttcacgt
     4441 tccggctgac cattctaagc gaggagacag ttttcataag gaggtagcaa tgtgttaatg
     4501 gcgttgaatc tatgaaacag cgttcatcgg ttgcggagag cagcttacgc cagccgggcg
     4561 cggcctggca agcgttcgtt gccggcgccg ccggcttttt cccgcctcag tcccgggcct
     4621 ccgcgcgcac gcgcttcagc cagtcctccg cgcccagcag gacggcgcgc atcatctccc
     4681 ggcgaaactc cggatcgagc atcttcgcct cgtccccggg atgcgtcatg aaagcctgct
     4741 ccaccagcac cgtgggcatc caggtgttgg cgcgcagcgg gtagtaattg aagttgccca
     4801 cattcccgaa atcattgagg cgggggatct ccagcagccg ggcgtggatg gccgcggaca
     4861 gatcccggca aaacggccat ttgtaatagg tgctcgtgcc cgacacgctc aagtagccgc
     4921 gcgcgtggcc cgccgcattg gcatgcacgc tgatgaacag gtcggcgtcg gcggccaccg
     4981 cccgccgcac gcgcgcgccc agggaaagag cttcgtcctg cgggcgaacc atcaccgtcc
     5041 gcgcgcccgc cgcctccagt tgccgcgcca gctcctcgac ggcccaccga ttgatgttct
     5101 tttcctggct gccgctgacg ccgcgcgcgc cggaatcgct gccgccgtgg ccgggctcca
     5161 gcgccaggac caggccccgc agcggcgagc cgggcgccgc ggccagcttc ggcgggcgcc
     5221 gcacacgcag catcaccgcg tcattggtca ccccccactg gtagccccac agttgcgcgc
     5281 cgttgagctc gacactgacc cggagatgcc cggcggccac ctgctggacc gtcacctcgc
     5341 gaatgacccg ggcggccggg cgatggctga tccacgtggc cgcgtgatgc gccccgtaga
     5401 aatcaatttc cagcgcggcg cgccccgcgg cgctgcgcgc gggcgtgacg gcgaacggca
     5461 ccggcgcgga ataggggatg gccaccaggt ccgccgcctc gttgccgccc accgagaggc
     5521 tggtaaacga caggtgcggc ggcggcgtgc ccgggggcgc ccattccacg gcccggcggg
     5581 caatccagcc gtcctggccc gggctgagcc ggacatgata attcccctcc cgcatccccg
     5641 tgacgcgcag cagcgtgccg gcgggcaatt ccgcaaggta ggggccgccg aggcgcacct
     5701 cgtgcagccc gaagctcagg cccgcgccct cctccgtcac ccgcaccagg cgcagccggg
     5761 tctcgtccca caagtccacc gttgcccggc ccgacacctc caccaccggc tcgccggtaa
     5821 cccggatacc gtccgcctcg ttcggccggg cccggagacg gaagcgcacc ggcgcattgg
     5881 tcgtgtccgc cgttgcgccg gccacgaacg ccgcccggta aaagccggtc ggctggcccg
     5941 tggccgcgtc gcgcgtctcg tgcatcggct gccaggcgcc ggccgacagg caatattcgg
     6001 cctcctgccc gggcgtgccg cggaaactca attccagaac atccccgcgc gacaggatgc
     6061 agttctgcgc cggctcaagg ctttccgcgt caatcgccag gcggcgctcc ttcggctccg
     6121 gcgccgcggc ggcttccggc tcgcgcttca cccgcacgac ctgctcatgc ttttgcccgt
     6181 ccggcgtcgt tgcgaccacc cggagctggt tttctcccag ttgcagcggc acgttgtcgc
     6241 gggcgaatat cccggtcgca aagacctccg ccgcctggcc ctccacccag accttcgacc
     6301 cgggcgcgat gcggcccagc acgtgcgcca ccgcgcgcgt ggtggtggtg ctctcgccgc
     6361 gcggatcaag aatccgcagc cccgcctccg ggttcgcttc ggccgccgcc gccgcggctg
     6421 ccgacgccgg cgcccacccc aaaacgccca gggccagcag cagcccccac cgggctcgcc
     6481 gggggacggg caggatggcg gcggcactac gggcgaacga cagattcggg atcgcagtca
     6541 tggcggcagc atagggcatg gttccggccg gtgtccatcg catccacggg gcggctcccg
     6601 ggggcattgg cgggccaaag acccgcgcgc ccgccggtga gcctttggcg cggcgtcagc
     6661 tatccccggg ttagcgggga ggcggggccg gctcgtgata cggtcggccg gcgcggtaga
     6721 gttcgagcag ttgcgcgttc cgttccgcta tattgtgctg cccggcggcg gtcgcgaggg
     6781 cgcgggcctg ctcggccgtc cggatggcgt cgtcgaaccg ggcggcttcg gcgtaggcgg
     6841 cggcgagcgt gcccagcagg acgggctcgc ggcgctggct cagttcgcag gcgcgttcgg
     6901 ccaggcgcac ggcttccgcg ccctcgcgca gcgcggcgtc ggagctggcg gcgcgaatcc
     6961 aggccaggtc attgagggct tccaccatgt ccggctgcaa ggccagcgcc tggcggtagc
     7021 ggtccaccgc cgcgcgcggg tttccctgcg cggccaggac gcccgcgtag aggtagagca
     7081 gttgaggatc ggccggcgtt tcctggagca tccgctcgta ttggacggcg gcttcgtccg
     7141 gccgcccgag cgaggtcagc gccagcgcca gcaggcggcg ggcctcgggc cactggggct
     7201 tgagcgccag ggccaggtga aggtgggcaa cggccccggg caggtcgccc gtctcggccg
     7261 ccgcgccggc cagcaaatga tgcagcgtcg ggttgaaggg atccacgcgc agcccttgcc
     7321 ggcaggcggc gatgacctgc cggggctggc ccatgaaggc caggcaggcg gcgagctgct
     7381 ggcccgccgt gggatggtcg ggcgcttcgg ccagcgcctg ccgcaggtgt tcgacggcct
     7441 ccgggtagcg gcggtccagg ctcaacagga gcgccgcctg cgtgtgggcc gcggcggcgc
     7501 gcgggtgctg ctggagggcc agcgccgttt cccgctgcgc gtccgcgtag cggccccggc
     7561 ggcggcaggc ctcggccagg cccacccgcg ccggcaggga agtcgcctcc agttgcagcg
     7621 cccgggaaaa cgccgccgcc gcctcgtcat agcgcccgtc atccgccagg cccacccccc
     7681 gcaccgtgtg ggtaaaggcg ttgccggagc gaatccagaa agccgtatag accgtcaggg
     7741 cccaggccag gacccccccg cgcagccagg gccgcagggc cggccatcgc cgggccgcga
     7801 aatcccagcc cacggcggcc accgcgcaaa gaggcagcaa cgcgggcagg gcgtaaaagg
     7861 ctttcacctg cgcgtaagac gccacgcgca ggctcatcca gccgatgccg gcggcaaacg
     7921 caaagagcag ccccagcatc aggaaccatt ccggggtcgg ccgccggagg aaccggacca
     7981 gggcaaccag ggttccggcg gcgatcaacg ccgtcgtgag cagcgccagc aggctgccga
     8041 tattcatcag gtcatagttc cacggcgggc gaaagttcat cagggcggag ccgctgcaca
     8101 gaccgtcccc ccagagcgtg gcgtagaggc cgtccgcgaa cccgtggatg ccgctgaaca
     8161 gcgggcaggt cagcgcctcg ccgcagcgct gataccacgc ggcggtgtgc gccccgggat
     8221 cctgccacca cgcaaacggc agccgggggt cccagttgcc gatgagcggc gtgccgaacc
     8281 ggtgccagac ccgggcaaaa tgccagccgc agaccgccag caggctgacc agaaaaaggc
     8341 ccgccgcgga ccccgcggcc cgcgccccgc ccgccgcccc gcgccgccag gccagcgccg
     8401 ccagcagaag gggcgccgcc agcaccgccg aaaacttggt cagcaaggcc aggcccaaac
     8461 cggcccccgc cgccgccgca agcccggcgc cgcgcgtccc ggccggcggc ggccgcagga
     8521 cgccataaag cgcggcggtg acgaacaagg cggccaggcc ctcattggtg atgtggtgcg
     8581 ccaggcagag gctcatgggc aggaacccgg ccagcagcag accggccacc tgcggccggg
     8641 gccgctgcgg aaacagttgg cgcaggcaca ggtagatcaa cagcaggtgc gcaaccccga
     8701 ccagcgtgga aaacgcgcgc agggtcagga tggccgcgtc cgccgaggcc gtccacccga
     8761 acggcccgat gagggcggcc gcggccaggt aatacagcgg cggctggtac atctgccagc
     8821 cgtcgtcggc cagcggcagc gcccgctgct ggaggatgaa atcaatgtac tgctgatggc
     8881 cgtcccggtc gaacccgaag agcgccgcca actggggcag gttgttgccc aggagcgcga
     8941 tccacgcgag gaggatcaac cccagcgcca cggcgggagc cggcggaaag cgggaacccc
     9001 gcgcccgcag aacccggtgg ccggcgacga gcgcggcggc gagcgccagc agcaacagcc
     9061 cttgcggcca ggcccggcgc agcgagtcgg ccgtccgctc ccggtcaaac aggggattgc
     9121 ccgcccgaat ggccggcggg cgcgacgccg gcacggccgc ctcccaggcc gcgtccacca
     9181 gcgagacctg ccaggcggcg tcgctgtaca gcgccggccc ggcgccttcc agcgccagcc
     9241 agagcgcggg caggccgtgc gcgttggaaa ccgtcacgga gatttcattc tcgcccgcct
     9301 tgaggaagcg ggccacgtcg gcggcgcggc ggcttttcca gtccgcccca tgcagcgcca
     9361 ggccgtccac cggctgcccg ttcacgcgca ccgcgccctg cctgaaaacg cgcacggcga
     9421 gggtggccgc ggcgggttgg gcggggagcg tgaaggcgcg gcggaactcg gcccagagcg
     9481 gcaccgcgtg gtgcggcgtg gtgtcgggcg gtttcggata gacaatccac tcggccgggc
     9541 cggccccggg gaggaagggg atgcggggat cgtgccggca ttgccagaca attccgccgc
     9601 cgccgagcag cagcgcgccg gcgatcagcc cggccagcgg gcggttccag gagcgcggcc
     9661 cgttcatggg gaaaccgccc ggccgggggc gcggacggcg ggcgggaggt tattcgggtt
     9721 catggtacgc ctggccggcc cggtaaagtt tcagcagctc ctcgttgcgt tgcgcgacgg
     9781 cggtcaaccc ggcggactgc gccagcgcgc gcgccttttc ggcggcgttg atcgcctccg
     9841 gaaaacggcc cgcctcggcg taggcggcgg cgagcgtccc gatcagcagc ggctcctggt
     9901 aggcggtgat ttggcaggcg cgttcggcca gcgtcaccgc ctccgcgccg ttgcgcacgg
     9961 tggcgtccgc gctggcggcg aggatccagg ccaggttgtt cagcgattcc ggccagtcgg
    10021 gctgggcctt gagcgcgagc cgcaaccgtt cgacggcggc ccgcgtttcc ttgcgcccct
    10081 ggtgcaggag cgcgagctgg taatgcgcca gcgcgttggt ggcctggagc cgggtggcct
    10141 cgcggaactg ggccgccgcg tcgtccgcct ggccccggtc ggccagcagc atccccagca
    10201 cggtgcgcgc ctcgctgtcc ccgggctgaa tcgccagcgc gcgctgcaac tcgccgatgg
    10261 cctcctccgg ccggccctgg gcgatcagga cattgcccag cagccggcgc ggggtgaaca
    10321 gcttgggatc cagcgccagc aggcggcgat aacacgcggc ggcttcctcc agcttgccct
    10381 ggccgtgcag cgccgtggcc aggctgtata gggccggcag gtgcaggggc gccagccgca
    10441 acgcttcgcg gcacggcgct tccgcctcgg cgaacttgcc ctgcttgacc agcgcgctgc
    10501 ccagcccgtt gagcgggtcg gcatacagcc ggtcgccctc ggccgccgcc cggtagtggg
    10561 cgatggcctc gtcgtaccgg gccagcgagg cgtaaacgtt gcccagggtg ttatgcgcct
    10621 ccgggtagtc cggagaaatg gccagcgcct tctggcacca cgcaatggcc tcggtgggct
    10681 ggttctgaat ggcgaattca ttggcgatgg ccgcgtaggc gacgaagttt cgcggcgtga
    10741 cccgggcggc atgctggaag agcgtggccg tgtcacgcca atgcgccacc tgccgggccg
    10801 tgagtaccgc gcaggcgccc aggaccagcg ccgccgccgc cgcgggaatc ccgcgcggcc
    10861 acgggcggcg ggcgatcacc gccgcggcgc cccagaccac gagcaggaac aggccgatgt
    10921 ggggcaggta agtgtagcga tccgccatgg cctgcttgcc cacctgcacc agcccgatca
    10981 ccggcaccag catgcccagg aaccacagcc agccgacctg caaggcgcgc cgcggcgggc
    11041 gggcgcgcca aatcccggcg gtcaccgctg ccagcagcag cccggcgccg gcggccgccc
    11101 acgaatcaag ccggaaaccg gcatagggat aaaagaccgc caggtcagtg ggccaaaagg
    11161 tcttgcccag gtagcgcgca tacgccacca gcgcattggc caggcgcagt tcgaaggggt
    11221 tatgcgccag cgacatcacc gcgccgctgg cccgctgcgc ctgaaaggtc accaggcacg
    11281 acagcgccga gagcgcaaag aaaggaacct tctccagcaa cacccgccgc agcggcgccg
    11341 cggaccgctg cccggacgcc agggcgagcc gctccagggg ccaccagtcc agcagcagca
    11401 gcacgcacgg cagcgtgacc agcatcggct tgctcatcag ccccagcgca aagcaaagca
    11461 gggccagcag ataaaaccac ccggcgccgc gcggagcggc gggggagcgg cgggccgtct
    11521 ccgcgctcgc tcgcgctttg ccccccggaa cggctcccgc ttccgcgggc ggcgccggct
    11581 ctccccgggc ggaaggggag gacggcggga gcgggccgcc gccggggtcg tgcgcctccc
    11641 gcgccggcgc ggagggccgc gcccccggct tccggttttc ctcgtccgcc ggctcgcgcc
    11701 gcccggcata tcgcgcatac gcccacagcg tcagcagaaa gaagaacgcg ctcagcacgt
    11761 ccttgcgctc cgcgatccag gcgacggatt ccacatgcag cgggtgcaaa gcaaacagcg
    11821 cggccaccag cgcgctgcgc cagacggcgc cggtcaggcg ttgccacagg ccgaacagca
    11881 ggagggtgtt ggccaggtgc agcagcacgc tcgtgaggtg atggccccac ggcttcagcc
    11941 cataaagctg gcaatcgagc atgtgcgaaa gccacgtcag cggatgccag ttgctggcat
    12001 ggaaactctg gcccgcccac gcgaggtttt ccagggtcag cccggattga acccgcgggt
    12061 tgccgctgac gtagtccggg tcgtcgtaat tgatgaagcc gaacttgagg accggccagt
    12121 aaaccgcggc aatcgccagc gccagcagca ggcaaagcag gagggtccgc cggggggcac
    12181 ccgccgacgt ccactcaaac tttcggcctt ccgccatatc aggcatgatg ttgccgccag
    12241 cccatgcggc gcaagcgcca aaacgacctt aagtcattgg tgaacgccgc ttgacactgc
    12301 cgccgcaacc gaactgtgcc aaattggcac acccatagcc agccgatggc atcctcccgc
    12361 caaccccatc gcccctggca aaataaccgc caaacaatgc ccgcaaccat gccaatttac
    12421 ccctcgcaac agcactcccg agtgtccaat tattggacac tccccgcaat atccgcctgc
    12481 tcaacccttt ttttcccacg aaccgcatct cctccttctt tacgcatttt ccgccatttc
    12541 ccgcccattc ctggctacca gcccgccgtg tacaaggggt gtacaaggga tgcccaccgg
    12601 atgttcatca gattttccga tgttcatccg gcgagcatcc cttgtacacc ccttgtacac
    12661 ggctatttgg gagggaggcg ggacggagag gaggggctga ggcgggcatc gggttgcggg
    12721 ggtgggggtc gggcggtttg cgcgcgatcc ggcttttcat gcgatttgaa gggttgatgg
    12781 ggttgaattg ggcggcgggt tcggcaatgc tggcgtcatg actgggttaa ccgatgtgct
    12841 gcagttgcgc gttcgttata gcgagacgga ccagatgggg actttttaca actcccgcgc
    12901 gttggaatgg ttcgagtgcg ggcggacgga gttgatgcgc cgccggctgg ggatgtctta
    12961 taccgtgctg gaggcgcggg gggtattttt gccgctggtg gaggcgcacc tggagtttct
    13021 gggcggcgcc cgctacgacg atttgttgga agtcgtttcc acagtgaagc tatcgggccg
    13081 ggcccggctg cggtttgagg tgcagattca tcaaagcgag agccgccggc cggtggtgcg
    13141 cggctatacg gttcatgcct tcacggatcg ccaggcccgg cccctccggc cgcctccctg
    13201 gtttctcgaa atgctggccg gggcgatgcc ggcgccgggc gcgccgccgt cataaccagg
    13261 gattgcctcg ccgccgtccc atcggctagg atggggccat cgctagaggc ctaaagttta
    13321 tgagcaaaca cgcgaagaac tcagagagaa acttccaccc gctcagcgcg ccggattttc
    13381 tgccgttgcc gcggttgcgg gacctgcaat tccagcggct gaaagccacg gtccagcgcg
    13441 cctacgaccg cgtcgccttg ttccgccagc gcatggagga acgcgacctg actccccggg
    13501 acgtgcagtc gctggacgac ctggccaagc tgccgttcac cgggaagaac gatttgcgcg
    13561 acacgtatcc ctttggcctg tttgccagcc cgatgcggga gatcgtgcgg ctgcacgcct
    13621 ccagcggcac cacgggcaag cccattgtcg tcgcctacac ccaggaggat gtgcaggttt
    13681 ggaccaatgt catgctgcgc agcctggccg cctgcgggct gcacgagggc gacatcatcc
    13741 agaatgccta tggctacggc ctgttcaccg gcgggctggg cgcgcactac ggcgccgagg
    13801 cgctgggcgc aacggtcatc ccgatttccg ggggcaacac gccccgccag ttgatggtga
    13861 tgaaggattt tggcgtcacg gccatctgct gcacccccag ctactttctc cacctgattg
    13921 accaggcgcc ggaggcgggc atccagttca aggacctgcc cctgcgcgcc ggcatctttg
    13981 gcgccgagcc gtggacggag gccatgcgcc gccgcatcga ggctgacagc ggcatcaagg
    14041 cctacgacat ttacggcctg tcggaaatcg tcgggcccgg cgtggccatg gagtgcgaat
    14101 gccaggcggg gccgcatatt ttcgaggatt acttctatcc cgagatcctc catctcaaga
    14161 ccggcaaacc gtgcgcggac ggcgaggagg gcgagctggt gctgaccacg ctgggcaaac
    14221 aggccatgcc gatgattcgc taccgcaccc gcgacatcac cgcgctggcc tccgaaacct
    14281 gcgaatgcgg ccgcacgctg cgtcgcatca agcgcatcgg gcgccgcgcc gacgatatgt
    14341 tcatcatccg cggcatcaac gtgtatccct cccaggtcga gaccgccctg ctcaaggtcg
    14401 agggcgccct gccgcattat cagattgtcc tgacccgcga ggaggatctc gatgtcatgg
    14461 aagtgcaggt cgaggtcacg ccggaggtct ttaacgacac ggtgggcgcg ctggaagccc
    14521 tgcggctcaa gctcaccaag tccctggaaa ccattgtcgg cgtgcgcgcc aaggtccggc
    14581 tggtccagcc gcgcaccatc cagcgcagcg agggcaaggc caagcgcgtc attgaccagc
    14641 gaaagatgta accccgagga aaatccccta tgaaggtaaa gcaactctca ttattcctgg
    14701 aaaacaaacc cggagcgctg agccggccca tcaagctgct ggccaaagcc cggctcaaca
    14761 tcctgacgct gtccatcgcc gacacccagc agtttggcat cctgcgcttt gtggtgcgcg
    14821 actgggaaaa ggccaaacgg ctgctggaaa aggagcagtt cgtcgtcaag gtctccgacc
    14881 tggtcgccat cgaagtagcc aacgagccgg gcggcctggc cggcatcctg ctcgcgctgg
    14941 agcgggcccg ggtcaatgtc gaatacatgt acgggttcac cctccgggcg gaaggcaagg
    15001 gggtgctggc gtttcggttc gatgacccgg acaccgccat cgcctcgctg ctgggcgcgg
    15061 gcatcaatcc cattcgccgc gtggacctgt tcaaccgcct cacgggttga ggcgccgtcc
    15121 cgctgccggc aaaggtccgt ttcgcatgcc gtccgggcgg cgggcaagcc cgccgcgcgg
    15181 gagcttccgc ggggcgcggc gcggtggaaa atattccgca tattttcctt gccaaacgcg
    15241 ccggcgcggg gctttaatac aggctgatta acaccgccgg agggcatgcg gacagggtat
    15301 gccgcaggct ttcggcaaaa cgaaaaacca acacctgaca tgaatacgat gaaacccatc
    15361 ctcacgcgac caaccctgcg cggttggcta ttggcgctgg cagcactcgc gctgtccagc
    15421 ttgatcgtcc atgccgtccc gtatgccacg gaggtcgtta aaaacggcaa taccgtgagc
    15481 tacattctga accaaaacgc ggcgagtgtg gaggtgcttc gcaatggcgg caatgcgctc
    15541 tatccgggca ccaacgccgg cgcctacaac tttgatatga ccggctacac caccttccaa
    15601 atcaaggtga tcggcaacga tgcgcccggt tgggtccagt acgttcccga taataaggac
    15661 gtcacgggct ttgagtaccc gtacggggtg tccgtcaata agaaccccca gagtcccaat
    15721 tttggcaaag tgtatgtgtc caattcccgg gtgggcacga ctgggctcgg ccgcagctgc
    15781 acgacggcca tttacatgct gcatgccgac ggctcggatg cggggttttc ggatggtggc
    15841 gtttactgga caccgggcgg ttccggccca tacaagagct gcatcggccc ggatgaccac
    15901 ctttacgtgg ccgatctttc caacgatttg gcctatgagt tcagcgatga tatgagcacg
    15961 gctacgctgt tgattgatga ctccaaccgg acgccgaacc agtacgtcca tagcatttat
    16021 gtggaaggca ctcaggcggc ggggaatcgc aagctgtacc tggtcaatgg caatatcaac
    16081 gatacggctc gcaagggcct catccagtac gacctcgggg ccaatgccgc tgccaccgcg
    16141 tgggacaccg gcacccagat tatcggcccc acgtactggg gaatctacta tagctatgat
    16201 gttgcgcgtg acagcaatgg cgactggtac atgccctcct accgggctaa tccgaaccag
    16261 cggccggcca tcaccaagtt cgacggatcc caaactccgc cgcttgataa ttacgtcctt
    16321 tgggagacgg ataagtcgct ttatctttac tcctttggca tagacatttg cgagcttaac
    16381 gggctggccg cctatggcca tgccaacaac ggcaatgttt acttcttcaa aatgaccgac
    16441 ggctcctttg tggaaaactt cgatgccggg ggggcgatcc gcgacctggc gtttgacgcc
    16501 gcgggcaaca tggtgacggt ggataacgcg gccgagtggg cgcggttctg gtcgccgggg
    16561 ggctacacgg tcgccaccac gacctttgat ggctcgcaga cgacgttcca ggttacccgg
    16621 ccgccggcgc aggtgagcgt gactgccagt caggcgacgg ttcccgaggc cgggccggcg
    16681 gttaacttca tcatctcccg cgtcggcagc gttgccgcgg atttgaccgt ctattacacg
    16741 ctgagcggca ctgcggacaa cggggtggac tacaccacgt tgtccggcag cgccgtgatt
    16801 cccgccaacg ccaactcggt gaatgtggcc ctggcgccga ttgacgatgc ggtggccgag
    16861 ttgagcgaga cggtgatcct gaccgtgatg gccaactcga actactcggt cgccctcccc
    16921 accagcgcca cgatttccat tctcgataat gaaaccccgg aaatctcctt cgccgcggcg
    16981 ccgccccgga agctgttgga gagctacgct ccatcgcggg tgacgcacca ggtggtccgc
    17041 aaggggttgt tgacgccgga gctgacggtc aacctgtcct actccgggac cgccacgcgc
    17101 ggggttgact acaatgcccc ggctgccgtc acggttccgg ccggagcggc aacagccaac
    17161 cttgtcttga cccccatcaa cgaccaggat tacgagggcg atgagaccgc caccgccaat
    17221 atcgcgggcg ggactggcta cacgattgga acccccagct cgattgacgc caccgtggtg
    17281 gacgatgatc ccccgccggg cacggtcctg ttcagcgata atttcgacac ggacagttcg
    17341 gccctgtggg aggtcaacct gtatgatccg tccgatgcct ccgttaactt cgcgtgggat
    17401 tacagcacgg tgggcatccc cgccgcgccg gcgggtggcg gcaccaaagg gctgcgcatg
    17461 cgctgcggca acacccagct catcttgaac gggttgagcg tttcgccgaa gaacaagagc
    17521 ttcaccggcg attaccgcct caagttcgac atgtggatta actacaatgg cccgatgtac
    17581 gatggcggcc ccggctcgac gcagaacttc gacgccggcg tgggcacctc cggccagatc
    17641 ccggtctggt tctgggcccc cagtcccgac gacagcgtgt ggttcaccgt cgcgggtgac
    17701 ggggcctccg gcttcacggt gggggattac aacgcgatga ttggggcgac gctcctgaat
    17761 gatgactcgg gcgtttacgc cgcgggcacg ggcgcgccga acagcggcat ccgcgatgcc
    17821 tcgaatgcgt attacgcgtt gtggggcggc caggcggcgc ccgcggcgca actggcgatg
    17881 tatccgaatc agaccgggaa cgcgaatatc ggcaacgcgg gcatggcgtg gcacacggtc
    17941 gtgattacca aagaaggcga cgtggtgacc tgggtgatgg acggcatcac gattgccact
    18001 gtgaccaata cggccctgcc gttcggcccc aacgtattcg tcggctacca ggacctgtgg
    18061 gcgagcgggt cgctgtctga cgttcccgaa atgtccttcg gcctggtgga caacctcaag
    18121 gtcatgaccc tggcggcgcc cgccgacatt gtcattaccg gcatccagtt gatcaatggc
    18181 caagcggacg tgcagattga cttcactgcc ggggcgggcg atgctccggc ggacttcacg
    18241 ctgcaggcca ccgccaacat cgcctccgcg cccgccgatg tcgcggccaa catcaccagc
    18301 accggcccgg gccagttcaa ggccgtgcgg gcggttgcgg gcaccgctca gttctaccgg
    18361 ctgcgccgga actgagccgc gcaattaaac gttcaggcga ggcacctgcg cgcgcgcagg
    18421 tgcctctgct ttttggcggg gcggctttcc gccgcggcgg cccggtccgc cgctcagtcc
    18481 ggaatcttct ccgacttccg atacgccagg ccctggaaga ccgccacgag gtcgcccgcg
    18541 tcgtccttta catccaccgt gtaggagccg agcttgaagt tgcgcgaaag ctcccgggcc
    18601 tcggcccaaa gcgtgccggc gcggccggcc ttcataaagg tgatgctgac attggcggcc
    18661 accgccaccg tgccgtgcga gttggacgcc gcggcaaacg cgaagtccgc cagggtgaag
    18721 atcgccccgc cctgcacggt gccgtagccg ttgaggtggt gcgggcccag cgtcatccgg
    18781 gcgcgggcat ggccgggcga caccgcgagc agctcgatgc tggcgtgcgc ggcgaattta
    18841 tcccccagga agaagcgccg gatgcggtcg agcgcgccgt caggatgcgc cgtgggagag
    18901 gaagcgggtg ggttcatgtc attttaagtt cgggctggct tgtttctgcg cttcgcgccc
    18961 ggtgaggaag gcctgccggt tgccggcaaa gaagttctcg gcaaacccgg cgcgcagggc
    19021 ggcgagccat tgctcctcgg tcatgggcag gtgggcgctc aaggccccca gcagcgccac
    19081 gttgagcgtt ttccggctcg gcaactggtc cgcgcgcacg gcgtccggcg taatcagcac
    19141 gcccccgggc cgcagcatgt gctgctgggg cgccacctgg gtcggctcga gcaccagcaa
    19201 gaaatccgct tcgcccgccg ccaccatcgg gctgaagacc tgcccgccga accgcacgtc
    19261 gccggtgacc gagcccccgc gctggctcat gcctttgatt tcgcttttct tcacatcata
    19321 gccggcgcgc agggccacgt ctgccaggat gtcggtgccg cggagcacgc cctggccgcc
    19381 gaggccggcg acgacaacat tggtcacttt ggtcataaga agggatgcgg gttccgggcg
    19441 ggctcagggg gaagccgggc tggccgcggc ggcgcactcg cagcgccgcg cttcgtattc
    19501 tttgagttgt ttggcgatca ggatgcacgg ccgccgggcg atgatgacgg ccagctcgcc
    19561 gctggcgagg cattcgtcca gcacgcgctc gaaatcgccc gagccgagcc gcggctcgac
    19621 cacatgcacg cgccggatgc cgagcgcgcg ggccaggtct tcgagcacca cctggcccgt
    19681 cggctcgtgg ccgagcgtgc ggccggtgcc cgggtgctcc tggtggcccg tcatggcagt
    19741 cgtggagtta tccaggatga tcagcacatg gcccaggggc ggcgggttgt acaccatctc
    19801 caccagcccg ttgatgccgc tatgcacgaa ggtgctgtcg ccaatcacgc tcaccacccg
    19861 ccgcgcctgt tcgggcggca gcacgtggcg caggcccagc ccgaccccca cgctcgcccc
    19921 catgcagacg caggtgtcaa tcgcctcgaa aggcgacagc actcccagcg tgtagcaccc
    19981 gatatcgccc gccacgatgc aatccttccg gcgcagcgcc tcgaagacga ttcgatactg
    20041 gcagccgtcg caaagctgcg gcggcttgcc gggcggcttg gacggttcgg ggctttcgtc
    20101 ccgctggaga atgcggcgca cgcgcggcac gtccagctcg ccgaaccgga accggtccgc
    20161 cttgccttcg accgggatgc ccgccgcgcg gatggcctcg accaggaaag ggtcgccctc
    20221 ctcgaccacc aggcagcgct ccacgccgcg ggcaaactgc gcgatgcgct tcaggggcag
    20281 cgggtgcgtg aagcccagct tgagcacgcg cgcctccggg gcggcttcgc ggacgtgcat
    20341 gaacgagatg cccgacgtga tgatgcccag cgccggggcg ccgtccacca ggcggttcag
    20401 ggggctggct tcgttccact cccgcagctc ggccagcttc tggcgcaagc gccggtgggc
    20461 gggccgggcg taggccggga tcatcacccg gccctgaacg tccttttcgt agttcgccgg
    20521 ccggggcgct tcggtttgcg cgcgcgggcg caccaccgta tagctgtggc agacgcgggt
    20581 ggtgacccgg aacaacaccg ggagcttcca gcgctcggag atttccaccg ccgcgaacag
    20641 gaagtcatac gcctcctgcg agtcggacgg ctccagcatg ggcagcccgg cggccacggc
    20701 atagtggcgg ttgtcctgct cgttctggct cgacgccatg cccgggtcat ccgccgagac
    20761 gacaatcagc gcgccggtga ccccggtata agccgccgtg aacagcgggt ccgccgccac
    20821 attcaggccg acgtgtttca tcgtgcacag cgcccgggcg ccgccgaacg cggcgccgag
    20881 ggccacttcc agcgccacct tttcattggg cgaccactgg gcgtgcccgc ccagggcgtg
    20941 gaaatgctcc aggatttccg tggacggggt gccgggatag ccggcgccca gcgcgacccc
    21001 ggcatgccgg gccgccaggg cgatcgcctc atcgccgctt aagagaagtc ggttggactc
    21061 attcattcga taaaatgcgc gcctgcttaa taacccattt ccgggccgcg ggacaacaca
    21121 ttccgcgccg cgccgcgcgg atttccgaac tggacaacgc ccggccgaac ggtgtacacc
    21181 cctttgcgca tcatgttctg ggatcaagca tcagaaacac tggcccggcc aacgctggaa
    21241 cacctgcaaa tcaaactcct gcaggaaacc cttcaccgcg tgagggagcg cgtgcccttt
    21301 taccgccagc ggctggccga gttgaacctt cagcccgcgg acatccgcac gctcgcggag
    21361 gtccggcggc tgcccttcac caccagcgac gatctgcgcg ccatttatcc cgatggcctg
    21421 ctggcggtgg accgcgccga agccgtccgg ctgcacacct ccagcggcac caccggcaag
    21481 cccaaggcca tcttcttttc gcgcgcggac gtggataacg ccgccgaact catcgcccgg
    21541 tcgctcgtct cggcgggcat caccgccgcg gacgtcttcc agaacatgat gagctacggc
    21601 ctgttcacgg gcggcctggt gatgcactac ggcgccgaga aggtcggctg cctggtcatc
    21661 cccgccggcc ccggcacctc cgaacggcag ctcatgctca tgcaggactt ccgcaccacc
    21721 gccgtgcata tcctgcccag ctacgccctc tatttcgcca gctacctcga acaaaagggc
    21781 ctgcgcccgc gccaggacct caccgtgcgc aaggcctttg tcggcgccga gccgcatacg
    21841 gaggaaaccc gccgccgcat cgagcagatc ttcggctgcg acgtgtataa ctcctacggc
    21901 ctgaccgaga tgaacggccc gggcgtggcg tgcgaatgtg aatacaaggc cggcatgcac
    21961 ctttgggagg acaacttcct gctggagatc atcaacccgg tcaccaacga acccctgccc
    22021 gacggccaga ccggcgaact ggtgctcacc accctccggc gggaagccat gcccatcctg
    22081 cgctaccgca cccgggacat cacctcggtg gtccccgagc cctgccgctg cggccgcacc
    22141 caccgccgcc tggcccgctt cgtcggccgc gccgacgaca tgctcatcat ccgcggcgtg
    22201 aacatcttcc cgcagcaagt cgagcgcgtg ctcatgtcct tcccccaggt gggccgcaac
    22261 tacctcatca tcgtcgaggg gctggacgac ctgaccatca aggtcgagtt gacccccgcc
    22321 gccttcgacg gccaactgga acacctcgaa gccctccagc agcagatcat cgagaagctc
    22381 cgggccgaaa tctggattcg gcccaaagtg gacctcgtgc ccgccggttc cctgcccgtc
    22441 ggcgaaggca aagccaagcg cgtggttgac aagcgttccc tctaaccgcc ccatcccccc
    22501 ctgttttagc ttatgcgcgt cgtaaacctg ttagccgtct ttgtggaaaa caaaccgggc
    22561 cagaccgccc ggatcgccgg gctgctcgcc gaggccggga tcaacctctc ctggctgacc
    22621 attgccaaca gcggcagctt tggcgtgatg cggttcctcg tggaccagcg cgacaccgcc
    22681 gtccgcgccc tcaaggataa aggagtcatg gtctccctgg tcgaggcgct ggccgtcgag
    22741 gtgcccgacc agcccggctc cctccaggcc gtggccgacc tgctgggccg caaccaaatc
    22801 aacctcgata attgctccgg cttcgtcgtc cgcgaccgcg ccatcctgat catcgaagtc
    22861 caccagattg cccaggccgg cgccatcctc gaacagcagg gcttccgcct gctcggccag
    22921 gaggaaatgc tgcggcttta agccgccggg ctcacgcccc gcccgggcgc ggcctccccg
    22981 cgcgcccgct atttgatgag ttcttcggca atctgcacgg cgttgagcgc ggcgcccttg
    23041 aggagttggt cgccgctgac ccagaagcaa aggccgttgt ccagggcgca atcctggcgc
    23101 aggcggccca ccgcgcaatt atacttgccc gacaggtaaa gcggcagcgg gtattcgctc
    23161 tgggcgggat tatcaatcac gtccagcccg ggcgccttcc ggagcaccgc cagcgcggcc
    23221 tcgaccgtca ccggccgctc gaactcggcg ctgatcgcga tcgaatgggc gcgatagacc
    23281 ggcacgcgca cgcaggtcac actcgcccgg aacgtgggat gatgcatgat tttgcgccct
    23341 tcgttctcca tcttcatttc ctcccgggta tagccgttcg gcaggaagga atccacgtgc
    23401 ggcagcacgt taaaggcaat ttgatgcggg taaacctcgc gcgtcaccgg ctggcgctgc
    23461 acaacctgct ccacctgccg ctccagttcc gtgatggctt tggctcccgt gcccgagacg
    23521 gcctggtagc tggaggcaaa aatccgccgg cactggaacg cggcgtgcaa cggatacagc
    23581 gcaatcagcg tgatcgccgt ggtgcagttc gggttggcga taatgccctt gtgccacttc
    23641 aggtcggcgg cgttgatctc gggaatcacc agcggaacgc ggtcgtccat gcgaaacgcg
    23701 ctcgaattat caataaccac gcagccggcc tgggcggccg ccggggcgta ttccttcgaa
    23761 atactgccgc cggcgctgaa gagcgcgatg tctatgccgc caaacgaatc cttcttcagc
    23821 tccgcgaccg ccagttcctg gccgcgatac ttgagctttt tgcccaccga acgcgccgac
    23881 gccagcaaag tcagcttccc ggccgggaag ttgcgctttt ccagcgtctt aatcatctcg
    23941 atgccaaccg cgcccgttgc gccgaccacc gccacatgtg gattgcgatt catataagga
    24001 gccagaatat agtattcatg ggccaaaaat gtcaatgtcg gcccgtagcc ccgagcgggg
    24061 ccgggcctca atcccagccc caagcgcctt aactcagccc gtccgccagc ctttcccaac
    24121 ctaaatccac gctaaatatc tctcgtcacg cggtttatgg ccattgccgc tccggccata
    24181 accccctgaa ataccgcttc caacaggcca tgtacaaggg gtgtacaagg ggtgtacatc
    24241 gggtgctcac cgggtgagca agggaaaatc cgatggacat ccggtgagca tcccttgtac
    24301 accccttgta cacggcgtca ggcgaggggg ggaggacggc ggcaggctgc cggaacgggg
    24361 gcggctggaa tgcggggatt ccgcggatca ggcggcggtt ggcggcaagg cggggtagtc
    24421 ggtatagcct ccggggccgg aggcgtagaa ggtttcagga accggggtgt tgaggggggc
    24481 gttgagggca aaccggcggg ggaggtccgg gttggcgatg aaaagcttcc cgaaggcgac
    24541 ggcgtcggcc tccccggcct ggagtaattg ctccgccgta tcacgggtat gggcttcgtt
    24601 ggcgacgtag aggccgccga actgcttttt gagcgcgggg ccgaggcggt tggggccgat
    24661 ggactcgcgg gcgcagataa aggcgatgcg gcgtttgcca agttcccggg cgacgtagcc
    24721 aaaggtggcg gcggggtcgg aatcccccat gtcgtgggaa tcgcagcgcg gggccaggtg
    24781 catggcgacc cggtccgccc cccagacggc gatgatggcg tcggtgactt cgagcatgag
    24841 ccgggcgcgg ttttcgatgg ggccgccgta gtcgtcggtg cggcggttgg tgctgtcctg
    24901 gaggaattgg tcgagcaggt agccgttggc gccgtggatt tcgacgccgt caaagccggc
    24961 gcgctgggca ttttgcgcgg cctggcggta ggcgtccaca atgccgggga gttcgtccgt
    25021 tgccagcgcc cgcggggtgg caaaggggcg tttggggcgg agcaggctga ggtgcccccg
    25081 ggcggggatg gcgctgggcg ccaccggctg cgcgccgccg aggtagcttg ggtcggaaat
    25141 gcggccgacg tgccagagtt ggaggaaaat ccggccgccg gcggcgtgga cggcttcggt
    25201 gatcgttttc cagccttgca cctgttccgg ggaccagatg cccggcgtat cggggtagcc
    25261 gacggccata ggcgagatgg cggtggcctc gctgaggatc aggccggcgg aggcgcgctg
    25321 gacgtagtag tcgcgcatca ggaggttggg cacccgcccg gcggaagcgc ggcagcgggt
    25381 caggggggac atgaagacgc ggttgggcag gtccagcccg cctaaccgga tcggttcgag
    25441 gagtcgaagg ccatggttca tattcatctc tttgccgggt tcggcgtttg gtaaagtcat
    25501 cggcgacagt ataggccagg ttccgggaaa tacgagccgg attatccggg ctgtcttttc
    25561 tcctttgcgt ttgcgggcgg ctcgcgttta agctccggcc atgaagattg ttttagcgta
    25621 ttcgggtgga ttggacacgt cggtgattct gtcgtggctg aaggagacgt atcaggcgga
    25681 gattatcgcc tattgcgcca atatcgggca ggaggaggag ttgaaggggc tggcggccaa
    25741 agcccggcgc accgggggtt ccaagtgctg cattgcggat ttgcaggagg agtttgcgcg
    25801 cgatttcatc tttcccatga tccaggcggg cgcggtgtat gagagccagt attttctggg
    25861 cacgagcatc gcccggccgc tcatcgccaa ggggatggtg gaggtggcgc gcagggaaaa
    25921 ggcgcaggcc atcgcccacg gcgccacggg caagggcaac gaccaggtgc gcttcgagct
    25981 gacggccgcg gcgctggcgc ccgagctgga ggtgatcgcg ccctggcgcg acgcgcggtt
    26041 ccgggccgag ttccccgggc gggccgagat gatcgcctat tgcgcgaaga agcggattcc
    26101 cgtgcaggcg tcggcgaaaa agccgtattc gatggaccgc aatcttctgc atatctcgtt
    26161 tgaggcgggc atcctggaag atccgtggct ggatgcgttc gcgccgggca acaaggacat
    26221 gtttgtgctc agcgtcgcgc cggaagacgc gccggataag gcggaatacg tcacgctcga
    26281 tttccagcag ggcaactgcg tggccgtcaa cggccgggcg ttgagcccgc tgggcgtcat
    26341 gcgaacgctc aaccggctcg gcggcaggca cggggtgggg cgggtggacc tggtggaaaa
    26401 ccgctacgtg ggcatgaaat cccggggcgt gtatgaaacg cccggcgggg cgattctgca
    26461 gtttgcccat cgccagatgg aaacgctgac gatggaccgc gaggtgatgc acctgcggga
    26521 ttcgctgatt ccgcgctacg cggagctggt gtattacggc tattggtttt cgcccgagcg
    26581 gctggcgctg caggcgttcg tggccgagag ccagaagaac gtgaccggca ccgtgcgggt
    26641 gaagctctac aagggcaata tcctgtgcgc cgggcggaag tcgcccgtga gcctctacca
    26701 tcccgccatc gccacgatgg aggccgaccc cacccaggcc tataaccagg acgatgccac
    26761 cggcttcatc cggctcaacg cgctgcggtt gaaagtggcg gcgcgggtgc agcggaagcc
    26821 gcggcccgcg gcccgccgcg gctagccgcc gcgcgtcacg tagccgtttt ggcgaaacca
    26881 cgcgacggcg tcggcaagcg cctgccgcgg gggcgtttgc ggcaggccca gctcgcggat
    26941 ggccttggcc gggttgaaga acatcttgta gcgggccatc cggaccccgg cgagcggcgc
    27001 tttggggggg cggtgcgtga gccgggccag ggcttcactg atgccggccg cgcccagggc
    27061 cacggcgtgc gggattcgca gccggggcgc gcggatgccg gtgatttcct ccagcacggc
    27121 aaacgcctgg cgcatcgtcc agttgccgtc ggcatggccg agaatgtagc gctcgccgat
    27181 gcggccgcgt tgcgcggcga ggatgtggcc ggcggcgaca tcgcggacgt ggacatagtt
    27241 caacccggtg tcgaggtagg cgggcatttg gcggttcagg aagtccacaa tgacctggcc
    27301 ggtgggggtg ggctttacgt cgcgcgggcc gaccggggcg ctggggttga cgatgacgac
    27361 ggggcagccg tcgcgggcca ggcggagggc cacttgttcg gcctgccatt tggatttctt
    27421 gtagtggttg accatctggc tttcgcccgc caccgcggtt tcgtcaaccg gcggggttgg
    27481 ccccgctccg ggttccggca gggccaggca gcccaccgtg ctggtataga cgatgcgctg
    27541 gcagccggcg gcggcggcgg cctggatgac cgtgcgggtg ccctcgacat tggcggcgta
    27601 catgggccgg tagtcccgca gccaaaggtg gtagctggcg gcgacatgaa agcaccattc
    27661 gcagccgcgc agggcggcgg tgagccgggc ggcatcgctc aggtcgccct cgagccgctc
    27721 gaactccgcg cccgccaaac cccggaggtc gcagcccggc cgcagcagcg ctttgacccg
    27781 gtgctggcgc gccgccagtt catgcaccag gttcgctcca atgaagccgg aagcgccggt
    27841 aacgaagcag ttcatggccg gagcttagga ttttccaccc cggcgaagaa gcagaaaacc
    27901 cggcgcggtc ccaaggctcc gcgcgcacaa aaaccgggac ggcggggggc cgtcccggtt
    27961 gcgggaatgg gtaagaaagg gattacgccc gcgcttttcg ggctttctta tcgccgatcg
    28021 ggcctctctt ttcccgggcg cgttgcatca tttcctcgcg cttcttgaac tgttcatcgg
    28081 tcaggatggc cttgagtttc ttggtttcct cttcgcgcag ggttttgatt ttggcggggc
    28141 gctcttccgg cttcagttcc tttattttct tagccgtttc ctccagcacg gccttgactt
    28201 tcggcttttg ggcatcggtc agcttgagct gggtggtcat gcgctccagg cgctgctcga
    28261 ggttgggcgc ttcacgtttt ccgcctttcc aggcggggtt tttggctccg ccctgggcgg
    28321 gggccgcgtt gcacgcgatc agcgcaccca gggccgcggc tgcgatcaga ctaagtttct
    28381 tcatgttgat gatcctcaat tttcgattgt attgttgccg gcctccgctt tatcgggcgg
    28441 caggccgatt ggttatctca aggaactaag acgtgtggga aaccggatgg ttacaccgaa
    28501 atgcgccgcc cggttttccg gggcgggcgg ccggggcgcg ccttttccgc cccggggtgg
    28561 gccggggcgg gcggaaggcg ggcgggagga aagcttcagt ggcctttgcg atttggcgaa
    28621 ttccgggcgt gacttggccg ccggtttatg ggaagctcga tgcgtttatg aagatagcga
    28681 cgacatggtg tgcttttagt ctggctttgg ggttggttgc ggcccagggc cagggaacca
    28741 ataacccaac caaagcggaa accaagccgg cggagcaagc cggcactcaa caaaagaaag
    28801 acccggtgaa tacaaaagaa gtagcggtga ttaaaacaac agaaggcgaa atggtcatcg
    28861 agttttggcc ggaagtggct cccaggacgg tggagaactt caagacgctg gcgaagaaag
    28921 ggttctacga cggcacgtgt tttcaccggg tcatcaaggg tttcatgatt caagggggag
    28981 acccgctgac caaggatccg gccctggaag cccgctgggg caccggggat cccggttaca
    29041 agattaaagc cgaattcaat gagcgccacc atgaccgcgg cgtgatctcg atggcccgct
    29101 cccaggaccc cgattccgcc ggcagccaat tctttatctg ccatggcaac ccgcggttcc
    29161 tcgaccggca gtacacggcc tttggcaagt tgatcaaagg cgacgacgtg ctggaaaaga
    29221 ttgccacgac ggcgacgcat cccccggatc ggcccaacca gcgcatgggg atcgagagca
    29281 tcaagattgt tgcggcggac tccgtaaagt agcgccgggc gggccggaga ccttgcctgg
    29341 cggccaacgc cccgcggggc gcgggcaacg gtgcttgcgt cggccggctg ttgcctttat
    29401 taaatagcgg tggctgcggc gttgtcgtgg ccggggttgg tagctcagtc ggtagagcag
    29461 cggcctttta agccgttggt cctgggttcg agtcccagcc aacccaccat tttccgcccg
    29521 gctgcgggct gaggccccca ggggcgcggc tggccggctt gacgggggag agccccgcca
    29581 caaccttttt cccggtgtca gacaaaacag cgtcggcgaa ctccgttccc gggcccgcgc
    29641 ccgggggagg gagggattgg gcttcgggcc cggctcggtt cgggtggggc ggcgcggctc
    29701 gtgttttcag gcgcgcgctt ccctggcttg cggccgggct gttgatgctt gccttcctga
    29761 agtttcctcc ctgctcggag ccggatgaag agcgggccta ccgggccgat ccttcccaga
    29821 cggccgtttt attctatgcc cagcagcacg ggctgcagtt tggcaaggac attgcctata
    29881 cctatggccc gctgggcttc ctgttgatgg attactttta tccctgggcg gcctggctgc
    29941 gcctggtggt ggaggtgggg ctggatttca tggtggcgct ggggatttgc cttgtggcgt
    30001 ggcggttgca ctgggggtgg cgatgcctgc tgctgggggg ctgcgcgtgg ggcgccgcca
    30061 atatcttcgc gggggatatt catccccggt gtgatttggt catcaatgcc gggcttctat
    30121 gctgggggtt gcttggcttt gtggaatccg ggcgccgcct ggcgtgggtg acggcggcgt
    30181 ttggcgccct ggccgtgttc tgcgccctgg caaaggtttc ctatctcttt ctggccgcgg
    30241 ggctggtgcc gttgctgagc ggcgacttgt ggctccgcgg acgccggcgc ctggcgctgg
    30301 gattgggggc gggttgcgcc gggggttttc ttctgggctg gaagctggcc gggcaaagcc
    30361 tggcgtactt ggcgccgttt ttggccaatg ccgtggccat ggtaagggct tacaatgccg
    30421 cgctgggctt tgagccgctg ctctgggccc ggaacctggg gtgggttctg gcgccgttgt
    30481 ttttggcgct cctgtggctc cgcgcccgga gcgcctttga cggcccggcc gggagagtgt
    30541 ggggccggcg cgggttcttg ctggcgtggt tgctgttgct gacgtttgcc gcctggaaac
    30601 acgggttcgt gcgggcctat gcgggctacc tgacgctgtg cttcttttat ttggccctgc
    30661 tcgccgtggc gctggaagtg ttcgtcgccg gaaaaccggg gcgcgggcgc ggggatgcgc
    30721 tgctgagcgg cgtctgctgg ttcttgccgc tggcgggggc gcaggccctt tcctttccgg
    30781 cgtttccggg ctcggtggct gtggcggtcc gcgcggccgg cgccaacctg ccggcgttgc
    30841 tgcggccggg ggagtatttg cggcgtgaga acgccgcttt tgcggagaac cgccgccggg
    30901 cgcagttgcc caggttccgc gagctcatcg ggagcgcccg cgtggacgtc ttcggctatc
    30961 gccaggcgtt tgccatcctc aatgatctca actatcgccc gcgcccgaat ttccagagct
    31021 acggcgcctg caacgccgat atgatgcgct tgaacgagcg gttctacctt tcgcgcgccg
    31081 cgccggagta tgtgctgttc gagtttctgc tcctggaccg caaatggccg tcgctggaag
    31141 acggatatgt gctgcgccag ctcctgatga attatgagcc ggtcgccacc gagcagaact
    31201 tcatcctgtt ccgggctcgc gccgccgacg cgccccggct gacgctgttg cgggagggga
    31261 ctgcgcggcc cggcgacgcg attgatctga gcgtgggcgg cggcgcaaac ctgtggctgg
    31321 agatagacct gcggccctcg tggagcgggc gcgtccggca attgctggcc cgcccgccgc
    31381 cggtgcggct ggcggcctgg cgcgagctgc gccgggggct gctcctccgg aagcgcgccc
    31441 cggcttcgat gctggcggcg ggtttcctgg ccagcccgct gctccagcgc aatccagact
    31501 tgctggagct ttatgcggcc catgcggcca cccggccccg ggcgtattcc gtggagttgc
    31561 tcccgggcga tgaacggttt tggcaagtcc cctttcgctt tcgcatttac cggatcgaga
    31621 atccgttggg ccggagaacg cccaatccct gacccgcccc ggggtgtgga tgcccttccc
    31681 ggcaggcaag cccgccgagc gctgaatcag gggcttgcca gccgatggaa gccgaataaa
    31741 ctgccgccat gagagcgaag aattggtgga ggtggttgct gttacttttg ttagtcattg
    31801 ggacgtttca attgccagct cagcaaagtg aggcggaccg caaatggctg gctgaggtcc
    31861 gcgccaaggc ggaaaagggc gatgctcatt cccagtgtga gttgggccgt gcgttcgctt
    31921 ttgggaaccg tggcgtgacg caggatgaag tggaggcggt ggagtggtat cggaaagccg
    31981 cggagcagaa cctcgtcaag gctcaattct atctggggat ttgctactcc cagggtcaag
    32041 gcgtggcgaa ggatgaagcg gaggcctata agtggtttct gctggcggca ggccaggggg
    32101 ataagggggc aataaaacag gtggacaaat tgaaaagaag catgacgcgg cggcaaattg
    32161 cagaagggca aaagcgggcg cgcgatttta aaccgcgaga ggcgccctct tccgggagcg
    32221 ataattccat cccgcgcatc ctggagacac gcccggaagg ttccggcacc ggattcttca
    32281 tcacggagga tggatatctt attaccaatg aacatgtggc cgggaacggg gcgctggtgc
    32341 gggtggttac ccaggatggg attctctccg ccagggtagt gagggtggat tcggccaatg
    32401 accttgcgtt gctcaaggcg gagggcagat ttgcgccgtt gccgctggtg tccagccgcg
    32461 acgtgaagct gggccgcacg gtggccacgg tcggtttccc taacatcggc ctgcaagggt
    32521 tcgcgcccaa gctggccaag ggtgagattg ccgctctttc cggggcgcag gatgacccaa
    32581 ggtatttcca gataagcgct ccggtgcaac ccggcaattc gggcggcgcg ctggtggacg
    32641 agcgcggcaa cgtggtcggt gtggtgacag ccaagctcag cgcgaagacg accctggcgg
    32701 ccagcggggc gctgccggag aatgtgaatt acgccgtcaa gagcagcttc ctgctcagct
    32761 tcctcgaatc cgtgccggaa gtggccgccc ggctccggga gcccaacctc aaagaccgca
    32821 agttcgagga cgtggtaaag gccgccgagc aagcagcggt gctggtgttg gtatattgac
    32881 cgggcgcaga atggttgagg gctgttgaat agcgcgagtt gcgcccgggc agtttgtggt
    32941 ggggaaacca ccttgccgag cggcgggctt atgcgaaaaa cggctggggg gaggattcgg
    33001 tttagggaag gacgcgcgcc gggaggcgtc cccgcaaaga ttcctgcctt cgcgctttac
    33061 tcgacggagg agttgggatt gtatgtggcc ataatgacag gcaagcactt cagaaggccg
    33121 gggcatgcgt aagggcgcgg cggtaatcga ggggtttctg gccaagttca ccgtactgaa
    33181 gggcgcttcg cgcgagttgt ggattacctt cgccgtgaag ttcctgggcg tcatcgctta
    33241 caaagtaacg gtgctgacgc tggcgctctg gttgatggcc gatttcgggt atggcgacgc
    33301 ggacgcgggg agcctggtgg cggggtggtc cattttgatg acggtcgtca cgctgctggt
    33361 ggggtcgctg acggatgcca ttgggttgcg gcggacgttc tttttgggtg tctggcttag
    33421 cgtggcggcg cgggcggtga tgacctttac gacgattaag tggctggccc tggccgccgg
    33481 gttgtttcca ctggcggtgg ccgaggccct gggcacgccg gtgctggtgg cggcggtgcg
    33541 caaatactcg accacgaagc agcgttccat ttcctactcg ctgttctaca ccatcatgaa
    33601 cctggggttc ctgctggcgg cgtttctgtt tgatcacctg cgccagagcc tgggcgagca
    33661 gggccggctt acgctgccgc tggtccactg ccaactgacc acgtatcgca ccctgttctt
    33721 tgcctcactg gggatacagc ttgccattct gccggtgatt tacttcatcc ggcgcgatgt
    33781 ggaagtcacc gacgagggca tccggttcca gccggccgcc agggggccgg tggggctggg
    33841 cttctggcgg gtctgctggc aaacggtgcg ggacagcgcg cgggacacgg tgaaactgtt
    33901 tggcggtctg ttgcggcagg ccgggtttta tcgcttgctg gcgtttctga tgttgatttc
    33961 gttcctcaag ctcatcttca tgcagatgga ctatgtcttt cccaagttcg gcatccgcga
    34021 actgggcgag ggggcgccgg tggggcgcct ggtggccatc aacaacattc tgatcgtctt
    34081 tttggtgccc ctcatcgggg cgttgacgca gcgctttacg gcgtatcgca tggtgatcgt
    34141 gggcggcctt atttcggcgg cctcggtttt tatcatggcg ctgcccacgc tctggtttca
    34201 accgctcgcg aacggctggg tggggcactg gatcgggcat ggttacctgc gattaacggg
    34261 cgaagtgcac ccgtattatg tgatgattac gttctttgtg atcctgatgt cctttggcga
    34321 ggccttttat tcgccgcgtg tgtatgagta cgccgcggcc attgcgcccc gggggcagga
    34381 agcctcttat gcggcgcttt cttacattcc ctttctgctg gccaagctga tcgttggcac
    34441 ggtttccttc cggctgctgg cccggtactg cccggaggtc ggggtgcgga attcgggggt
    34501 gatgtggctg gtgatcggca ttattgccgc ggttgcgccg gtcgggttga tcctgctgcg
    34561 gcgttttatt cgcgtgcagg aagccggacg ggagggttga cgactcccgg ggcgaagccc
    34621 gggcggaggc ggagtggttt tgcgcgggcg gggcgctaac ttacggccgc ttcggtgatg
    34681 accaggtcgc cccggagggc gtcgagggtt gcgcgcacgc cgagcggcaa ggtggcgttg
    34741 tgggaaacgt ggccgaaagg caggcccatg acgaggggca ccttgagcgg gagcagccgt
    34801 tcccggagca catcttccag ggtctggcgg tattcccggg tgcctttggc tttcgggtct
    34861 ttgcagtcgg cgttgacgcc aatggcgatg ccggcaacct gttggagaaa gccggcgtta
    34921 agcagttggg tcagcatccg gtcaaagcgg tagggcgctt cctcgacgtc ttccaggaag
    34981 agaatgctgt ttttgacggg gggctgtccg ggagtggcca gggcggcgca gagcaggctg
    35041 agattgccac ccaccagcgg cccggaagcg atgccgcggc gcaggatggt gacggttttg
    35101 cgccggtagc cgcggcaaat gctgccgggg ggagacggct gcatcaaggt tcggaggaag
    35161 ccctgccgcg tgaacgaggg caagtccggc ttcacgaaat ccgagttgag catcgggccg
    35221 tgaaaggaga cgagccgggc atgggtcagg aaggcgcaat ggagcgaggt aatgtcgctg
    35281 tagccgacga agattttggg attggcgcgg atgaggtggt aatcgaggag cggcagcagc
    35341 cgtgcggagc catagccgcc gcgaacgcaa aggatggcct tgaccttgcg gtgagcgaac
    35401 atgcgcataa ggtcgctggc gcggtcgcgg tcactgccag ccaggaaacc gtggcggcgg
    35461 cggacgttgg gggcgagcag gggcttgaaa cccaagtgtt ccagggccgc ggcggcgcgg
    35521 tcaatggttt tcggatcggg cggcgcgctg gcgggggcga cgattccaat cgtatcgccg
    35581 cgggttaaac ggtctggctt gatcagcatg ggcgcgcggg cagcggttat tggttgcgaa
    35641 gggagagaag ctgggccctg gtctgggtaa aaacagagtc gagttcctgg accaaagcgg
    35701 ggacgccatg catattccgg gcgcggccaa gatcttccag cttctgggaa atctcggcga
    35761 ggcgggtggc gcccaggttg agggcgctgc ctttcagcgc gtgggcatga aaggtcagca
    35821 tcgggccatc gttaacggac tggttgattt ggatgatgcg ctggggggcg ttctggagaa
    35881 acaagtccac caactcctga aatacgccga cgctgccggt ggcgggcagg ttgcgcaatt
    35941 cggcaatgag ggcgtagtcg agcagctttt cgctcgggat gacgcgcgcg cgggccagca
    36001 gcgttgtatc gcttcttttg gggcgggtgg ggccccagcg ttcaatcgca gattgcagat
    36061 cgccaacccg gaccggcttg gagatgtagt cgtccatgcc cgcctgcagg catttttcgc
    36121 gatcgcccac gaaggcgttg ccggtcatgg caatgatgcg cgggcgcttg tcagccggcc
    36181 agcgctggca aatctggcgc gccgcttcca ggccgtccat ctccggcatt tgcacgtcca
    36241 gaaacagaag gtcgtagggc ttttgtccga gcgtttcaag cacctcccgg ccattgctga
    36301 caacgtcggc gcggtagcca agcttgctca gcacgctcag gccgaccttt tggttgatgg
    36361 gattgtcatc ggccagcagc acgcgcaggg gcagcttgtg ggcgagggtg gggtcgagcg
    36421 tcggggtcgg cggtggtttt ttctcacgca gggtctggac cttcaaggcg tggcacaggg
    36481 cctccagcaa ctgggccggc cggatgggtt tatgaatggc aacagccatt ccctcgcgca
    36541 cggggtgggc atcctcgccg gacggacgca tggccgagag caaaagcagg gggacgcaac
    36601 gcccggaagg cagcttccga atctgctcgg ccagcgccgg cccttcctgt cccggcagtt
    36661 cccagtcgag gattacggca tcgaacagat cgccttgcgc gagcagggcc agggcttcgc
    36721 ggcgattgtc ggcggtttga actttcatgc cccattgctg cccgcgatgg gaaatgatgc
    36781 gccggttggt cgggttatct tcgataacca gcacgcgccg gcccgcaagt tgcggctgcg
    36841 ggtttaacca ggcgggcggg gtggcggcgg cgggcgcccg ggtggagatg gtgaagtgaa
    36901 aggtggcgcc ctggccggcc tcgctctcga cccagatccg gcctcccatc aactcggcca
    36961 ggcgtttgga aatcgccaaa cccagcccgg tgccgccgta atggcgcgtg gtggcggcat
    37021 ccacctgttg gaaagacttg aagagccggc tctggcgatc cagcggaatg ccgatgccgg
    37081 tgtcccgcac ggagaaatgg agcagccaat gattgggatg gcggagaaat tccgtgtccg
    37141 gcgcgaagcc gggaggcagg ctgcgcgcgc cgtgttcgtc ggggaccacc tcgacgacga
    37201 cctctccctg ctgcgtaaac ttgacggcat tgccgatcag gttgaccagg atctgccgca
    37261 ggcgggtcac gtcgctgacc agaatccggg gaatggcatc gtccaccagg caggccaggt
    37321 cgaggttctt ctgggcggcc cggggagcca gcagctttat gacctcctcc aggcaggtgc
    37381 gcagctcgaa ggggcggtat tccagttcga gcttgcccga ctcgatcttg gagaaatcca
    37441 ggatgtcgtt gataatcgtc agcagcgcct cggcgctgtt gcgggtggct tcgacgtatt
    37501 cgcgctgctc gtcggttagc tcggtgtcca gcaacaggcc ggtcatccca atgatgccgt
    37561 tcatgggggt gcggatttcg tggctcatgt tcgccaggaa ctgggacttg gccgccgtgg
    37621 cgtcctcgag ctggtggttg agggaaatga gcgcggcgtt gctggcgatg agctgctgct
    37681 ggctctcgcg cagggcccgg taggctgcgt cgcgctgcga caggttgagg caggcctgcg
    37741 cgtggtgccg aatgcgggcc gccagctcaa tcttatcggg cagctttacg aggtaatcgt
    37801 tggcaccgag ggcaaaggcc cggcctttga cctgcggatc ctcgttggtg gaaaggacaa
    37861 tgatggaagt gtcccgggtg gccgggttgg cgcggagttg ctccaccagc gccaggccgt
    37921 ccgcgcccgg cattaccaag tcttggagga tcacgcaggg cttgatccgg ctggccatcg
    37981 cgagggcttc gcgggcatcg gcgcagtagt gcaggtccag gttcggctgg ccggccagcg
    38041 cgcgccgaat ggcctcgaag accagcacct ggtcgtccac aagcaaaacc gccacgcgat
    38101 cctcggcggg aaccttcgga cgcgccgcat ccgggcgggc ggtgcctttg ttgagctcgt
    38161 cggtcatgtt ttttagccag cgacgctacc ctaataggta gtgcagcggc tgattttgtc
    38221 gagtgttaaa atcttggcgc ggcgcgaggg gttcgggcgc gcgccgcggg cggcgctggc
    38281 ggcgcgcccg ggccgccgcc cgtccgcccg ggacctcagg catcgggcgc gccaatcaaa
    38341 tccgccaccg cctgcaggag cgcgtcatcc cgataggcgc tcttggtcag gtagaaatcg
    38401 gcgcccgtaa tccggccgcg cgattggtcc cgttcgcggt cctggttgga gatgatcatc
    38461 accggcaggg acttgagctg cgggtcctgc ttgatcagcg aggtcaattc gatgccgctc
    38521 aggcggggca tatccacatc ggcaatgacc aggtcgtact ggctggcgcg cacggcgttc
    38581 cagccgtcca acccgtccgt ggcgccgtcg gtcaggtagc cgcggctggc cagcagcttc
    38641 cgcatcagtt cgcggacgat cagcgagtcg tccaccacca gaatgcgttt tcgccggcgg
    38701 ccggccggcg cgggaggctg ggggggaacg agcttgaggg gctgcccggc cgccagtttc
    38761 cccagggagc gcagcaggtc gtccacgtcc acaatcagga cgggcgcgcc gttttccagg
    38821 agcgcggcgg agctgatgtt attgaccttg cccaggcggg catccagcgg ctgcaccacc
    38881 agctcgcgtt ctcccaggaa gcggtccacg agcacgccat agctcgcgtt gcgattgccg
    38941 agaaccacca ccgcccagtc gtcgtcgcgc ggttggggag cttcggcgtc aaagacctgg
    39001 tgggcggtca ccagggcgac ctgccgcccg gcgaaatgaa aatgatggcg gccctcaagg
    39061 gtttccaccg ccgcgcgcgg cagccgcaag gcgcgcgtga tctggtttag cgggaaggcg
    39121 taaggctcgc catccacctc caccagcaag gcgcggagca ccgagagcgt gagcggcaac
    39181 tgcaactgaa cctgcaagcc ctggccgggc tgggtgctga tacgcaaatg gccgcgcacg
    39241 ccgcggacca tgttctgcac cacgtcaagc ccgacgccgc gcccggaaat ctccgtcacg
    39301 gtttccttga gcgtaaagcc gggcaggcag aggaagtcga gcagttcggg ggccgtgaat
    39361 ttttcggccg cggccgggtc ggccaggttt cgctggcggg cggcttcgcg cacgagctcc
    39421 aacggaacgc cggggccgtc gtcggcaacc gtgatcagca actggccggc gctgtggcgc
    39481 gcttccagtc ggatggtgcc ctcgcggggc ttgccgaggc ggtggcgttg ctcgggcgtt
    39541 tcgcagccgt gatccaccgc gttgcgcagc aggtgggcaa gcgggctttc caatcgctcc
    39601 aggaggtccc ggtccaccgg aacgctctcg ccgatgattt cgagccgcgc ctttttgccc
    39661 agggaatggg ccaggtcgcg aaccatgcgc ggaaaccggc ggacgccatc gctgaaaggg
    39721 cgcatgcgcg tgcgcagcac ttcgaggtac aggcgctggg aaagctgcgc ggaacggcgg
    39781 tcaaacaagt ccagctcttg cagccggtcc gccagcaact gctggcacgc ggcggccttg
    39841 tgggccagtt cggcaagctg gtgccgcgtc cgcgcgggca ggcccgcttc atcgccgtgc
    39901 cggcgcagtt cttcgagttg ctgccacagt tcgttttgct ggcgtttgag gcgctggagg
    39961 gagtcggcaa acgggcgcat ccagcgcgct tccaccaggg actcgccggc caggccaagc
    40021 agccggttca ggttttcggc ggtgagccgc accacgcgct cgggcacgcc cggcccgggc
    40081 gggacgctgg ccggaggggg catctcttcg ggggcgggcg gcgcggtgtc cgggatggcg
    40141 ggcggcggcg gggcggcggc ggccgcatcg ccggcctggg ccaaatccgc cagcgcgcgg
    40201 agcagcgttt gggtttcggc ggccggcgcg gattccccgc cggcgcccgg agtctcggcc
    40261 tgccgggcga gctggagcag ctcatccgtg ccgcggagca gcaggtcaac ttccggctgg
    40321 cgcaggacca gtttgccccg ctgggccagg acgaagcaat cctccagcgc atgcgcgatt
    40381 tgcaccgcgg cctccagccc cacgatgcgc gcggcgccct tgagcgagtg ggcggcgcgc
    40441 atgagctcgg ccaggtgttc ggacgttgcc ggtttctgtt cgagctcgag cagcccgcgc
    40501 gtgagaatgg cgacctggtt ttcggcttcg acgcggaaca attcccgcat cgagagattc
    40561 gccagatcct cgggagggtc cgtgctcacg ccaggctccg gttcagggac gaaaagacgc
    40621 gctcggcatc gagcaggccg accacccggc cctgccaggg aagcaggccc cgggtataag
    40681 caagcccgga ttttgccagc gtggccggcg gttcggtcaa ttccgcggct tgaaacttct
    40741 ggatgccccg aacctcgtcc gcgggaaagg catagcgctg ccccgcccag ttggccacca
    40801 gcagccgatc gtaggccgcc tgccgcgctt cgggtcgcgg gggttcgtcc cgccgcagca
    40861 aacgggcgag cgagacgcaa agcagcaact cgccgcgcac attcaccagc cccagcacga
    40921 tcccctggcg tcgatggggc agcgaatgaa ccgggcggcg ttcggccact tcctggaaca
    40981 cctgcgccgg caaagcgagc cattcggcct ggaggcgaaa caacagcacg gctagtgacg
    41041 ccggggccgc cggcgtctgc gcctgcgcga aatgctccgt ccaggcgcgc cggtagtccg
    41101 gggtcagcgg gcggtccagc aactgccgcg cggcggcgga aaagaccggg caattgcggc
    41161 agcgaataac ctccgccagc ttcgggcagg tgccattgcc ctgcccgccg atttcattcc
    41221 agcaagccct ggggtccagg acggcggggg gcgaggctgg cgaatcggga gtcttcaccg
    41281 gagtggggcg tcttcccgcc ggaaatcatg gccgcctgtc cccgcttttg ggacgccacc
    41341 caaaacgccg gtcctccccg gtcaggcgga ttttcgcgct tccttggccc aggtatcctt
    41401 gagcgcgatg gtgcggttga acacccgccg gtccggcgta ctgtcctggt cgagcgtgaa
    41461 ataacccagg cgctcaaatt gatagcgcag ctccgggcgg gcgtccttca gccccggctc
    41521 gcatttcgcc gtgaccacct ccagcgagtg cgggttcagg tgctgcttga agtcgccgct
    41581 ggcgtcgggc acgggcacgg taaagagccg gtcgtagagg cgcacctcgg cgtccatcgc
    41641 atgcgcggcg ctgacccagt gaacggtgcc cttgaccttg cgcgcggccg tcggcccgcc
    41701 ggtcttgctg cccaggtccg ccgtgcagcg caattccacc acctggccgg cggcgtcctt
    41761 gatcacctcg tcgcacttga tgatgtaggc atacttgagg cgcacctcgc cgccgggccg
    41821 caggcggaag tatttgggcg gcggcgcttc catgaaatcc tcgcgctcga tgtaaagctc
    41881 gcggctgaag gggaccttgc gcttgcccgc gcccgggtct tcggggttgt tgacggcctc
    41941 gagctcctcg acctggccct cggggtaatt ggtgatgacc accttgaggg ggcggagcac
    42001 cgccagccgg cgcagcgcgc gccggttcag gtcttcccgg atgcaatact ccagcacgga
    42061 gacatcggtg atgccgttgt acttggtaat gccgatattg taagcgaagt cgcgcagcgc
    42121 ctcgggtgtg acgccgcgcc ggcgcaggcc gctgatggtg ggcatccggg gatcatccca
    42181 gccggaaacg aggttctcgt tgacgagctg catgagcttg cgcttgctca tcacggtgta
    42241 gcccaggctc aggcgggcaa actcatactg gtgcggcagg ggccggggca ggtcaagttg
    42301 ttcgagaatc caatcgtaga gcgggcggtg cacctcgaac tccagcgtgc aaatcgaatg
    42361 cgtgatgccc tcgagataat cgctcaggca gtgggcgaaa tcgtacatgg ggtaaatgac
    42421 ccacttggcg ccggcgtgat gatgctccgc gtggcgaatg cggtagagca ccgggtcgcg
    42481 catccagaca ttcggcgacg tcatgtcaat cttcgcccgc agcgtgcgcg ctccatccgg
    42541 aaactccccg ttcttcatcc ggacgaagag atccaggttt tcctcaatgg aacggttgcg
    42601 gaaagggctt tcctgcccgg ggcgatccgg ggcgccgcga tacttgtcgg tgtcttccgc
    42661 cgagagatcg tccacatagg caaccccctt cttgatgagc tgcacggcgt attcgtaggt
    42721 ctggtcgaag taatcactgg cataaaacgg ctccagcgga acctccgacg ccccggtcac
    42781 cggccccagg taaaaatcgt tcttgccgtt gacggtctgg gccaccggaa acttgccctt
    42841 gggcttgagg ccaagccggt cgtccgccca cccgcgaatg agccacttca cgtcctcgat
    42901 gatcgagtcc acatattcga cgtcttcctt ggtcgggttg gtgtcatcca tgcgcaggtt
    42961 gcaaaccccg ccgaattccc gggcaatgcc gaagttgagg cagatggact tggcatggcc
    43021 gatgtgcagg tagccattcg gctcgggcgg gaaacgcgta tggatccggg ggtatctctt
    43081 ttccgcgacg tgggcaatca cgatgtcgcg gacaaaatcc gacggcgtgt tcggcgcacc
    43141 ggttgcggcg gcaggttcag aagctttgtt agccatattc attttgtatg gaacgaagaa
    43201 taacaggacg gccgctcaat gtccaacacg tagccgtccc ccattccatc cctcccgatt
    43261 atgggaaata aacccggaat ccccgccctt tcgaaacccc aaccgctggg tcttttgaga
    43321 ccgcgaatcc ggcagccctg gcaccccggc cagccgataa tcatcatgga gccccaatgc
    43381 caaaactaat aattgcaacc cgcgttgcct ttccgatccc aacc
//
LOCUS       DAOPQG010000013        42149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1215_length_42149_cov_14.180786, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000013 DAOPQG010000000
VERSION     DAOPQG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 42149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42149
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_42149_cov_14.180786"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..1029
                     /locus_tag="PKI07_02540"
     CDS             250..1029
                     /locus_tag="PKI07_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC49959.1"
                     /translation="MNFGGRKWIAEWLVGLGRITLLAREAVGSLFRFKVAWRDFLYQV
                     HFVGVKSQSVVLITGAFTGMVLGAQTYFQFHKVKMDTAMLAVVSVSMCSELGPVLTGL
                     MVAGRVGAAIAAELGTMRVTEQIDALRTLATHPVDYLVVPRLLALHLALPLLTVEAIA
                     IGIGAGYLVGVFLLGVDPVYSWYNMLRYTSTGDVIVGLIKAVFFGGMIALVGCYKGLY
                     CGEGAEGVGRATTEAVVYSSIAILISNFFLTLTLGRLLNTL"
     gene            1026..1772
                     /locus_tag="PKI07_02545"
     CDS             1026..1772
                     /locus_tag="PKI07_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC49960.1"
                     /translation="MIEVRNLTKHFGSNPVLKDVSFRIEKGESVVIIGRSGCGKSVLL
                     KHLIGLLQPDAGRVLIESEDIVGMGERELLRVRRKFGMLFQSAALFDSMTVAENVGFA
                     FRRERALPSREIRQRVAEVLEMVDLPGIENKKPSELSGGMRKRVGLARAIIYRPAIVL
                     YDEPTTGLDPIVSDSIDQLILRVRDRLSVTTVVVTHDMRSTRRLGQRILMLHNRQIYA
                     AGTADEIFHSSDPIVRRFIEGVSDPKELYF"
     gene            1774..2763
                     /locus_tag="PKI07_02550"
     CDS             1774..2763
                     /locus_tag="PKI07_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOC49961.1"
                     /translation="MDKSRLEWKVGLFVFVGLVLAAGLLLQFSKGTTFFRPAYEIILE
                     APTVSGLKPRAQVLMSGIQVGSVSAMQLASDGKSILITLRIYQQYQIYKDARFVFEQS
                     GFLGDQYVAILPTQNQGEVFRPGDRAAAEAPLDIQKIARSAAGLLEHIDAAATNINHV
                     LSDARLTVLSARALTNLQTAFDNFHRVSERSLEVVENAGALLDTNRPTVSAALSNLLS
                     FSDQANEAAGDVRALLAANSSAITTSLSNIQSSTDTLKALLDNLKQGRGTVGKMLESD
                     EIAANVSQVVSNLSITTSNLNRLGLWAVLRKPRPAPTNPPARAVLTAPKNPFP"
     gene            2773..3786
                     /locus_tag="PKI07_02555"
     CDS             2773..3786
                     /locus_tag="PKI07_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOC49962.1"
                     /translation="MALWAIRILFLGLCTGGGYAISQVRSEWLGHGAWFLIAGFGFGW
                     LLIAIDEMLKGFSLRAFSATTFGLLLGTLVALLIDHSGLFGEPNDNSPHRLIVRLALF
                     VGFGYIGMVLAMRSNKEDFSLIIPYIRFAPQNKPDNLLLLDTSAIVDGRIADLIEANF
                     IEGLIVVPRFVLKELQQLADSNDPLKRARGRRGLDILNRIQRNTRNEVKIHDGDFTDE
                     AAVDAKLVRLARNLRAKLYTNDSNLAKIAELQSVNCVNLHEIAKVLRTVLLPGELLNL
                     RIVREGKDKGQGIGYLPDGTMVVVNNAQEAIGQQVQALVQSQLQTGAGVIVFADLKPP
                     ATT"
     gene            complement(4212..6659)
                     /locus_tag="PKI07_02560"
     CDS             complement(4212..6659)
                     /locus_tag="PKI07_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOC49963.1"
                     /translation="MKRPLVWIALVYIGGLLLAELYQPPLVPLFAISLGLGVGALALP
                     AARPWLIWALVLFTGWTNLTFRTALLSPHDLRLIVGSEPILATIRGTLTETPGHRTYV
                     RNEKETTRTIAQVRVSHLQCGNDAWQPAFGDVLVTTPTRLPDEIYARHVVQITGVLAP
                     PPRPVAEGLFDYCTYLRRHGIHYLLTASTTNDWQVLDGRRPRPLGDRFRAWARASLAR
                     GLPVEDEPLRLIWAMSLGWKPALTHETYEPFMRSGTMHIFAISGLHVALIAGILTRLL
                     RLLQVPRVGCGWVVIPLIWFYTAATGWQASAIRATVMMSIIIGGWALKRPSDLLNSLA
                     AAALLILLWDPRQIFTASFQLSFLVVLSIALLAPPLEAMRNRLLQSDPLVPADLLPRW
                     RRGLEFLLRGGTAAAVVSLAAWMGSLPLTAYYFHLFSPVTVLANLVIVPLSSLTLAAI
                     VGSLICGSWLPWVGECFNHSGWLWMSCMVKLSEWATQLPGAYYYVRGPAGWGVIAGYL
                     ILIGGTAGLFLPAKPRRWTLMGVAVLAGFYAWQWLTMRPPASLTVIPVNGGMALYYHA
                     SNQSDRWLMDCGSTNSVEFVTKPFLRAQGINSLPALILTHGDLRHIGGADVIADLFHA
                     RQILVSPVRFRSPKYRLLLQEFERKPQWLRRISSGDRFGPWKVLHPEADDRFGQADDN
                     ALVLLGNFHGTRVLLLSDLGRTGQNALLERTPDLRADIVVTGLPSQYEALSDALLEAI
                     QPRLIIVADSEFPAAERATAKLTERLACWKVPVIYTRNTGAATLELRPFGWKVRTMNG
                     DRFTSKSPKLKSNTAGQ"
     gene            complement(6726..8108)
                     /locus_tag="PKI07_02565"
     CDS             complement(6726..8108)
                     /locus_tag="PKI07_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOC49964.1"
                     /translation="MNPKKIKLLRRLQRLLSPGEISFDPSVLTQHAGDKWFATHQPDA
                     VVLARSTRSVATVLRFAHRHRIPVTARGAGHGYVGGCVPSRGGIVLSLARMNRIREIN
                     AADFVAVVQAGVNTARLQQAVEKQGLFYPPDPASRADNSIGGNILTNAGGPRCLKYGV
                     TRDYVLGLEVVLPGGDILRLGGRTHKNKTGFDLHRLFVGSEGLLGVVTEATLKLLPLP
                     PYRACLALGFASMRAAVRSLHAILAAGFLPAALELADSFTLAAAYRRTRSPRLRGCRA
                     HLIVELDGQTRSVRGEIRQLRALLAPRRPLFIQTAFGPAQCEALWQIRREFSYALRDT
                     GLTKLNEDIVVPRGRLEDLFAFAARLQRRHRLAVACFGHAGDGNIHTNLMVDFQQPGA
                     RNRARIALDDLFRQIIAWGGAITGEHGIGLAKLRWWPRALPPNVRALHQTLKQTLDPH
                     GILNPGKFLS"
     gene            complement(8144..9124)
                     /locus_tag="PKI07_02570"
     CDS             complement(8144..9124)
                     /locus_tag="PKI07_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOC49965.1"
                     /translation="MFGLRISQFGAALALLILAPAAGPTKVKAAAAKPGVQITKLADR
                     VRVQMHGQLFTEYFFKDVPRPYCYPLIGPGGAALTRDWPMKDTPGEAHDHPHHRSLWF
                     AHGAVNGHDFWSEQKGFGKTVHDDFLQIKSGRRQGLIRSRNLWVAADGRRVCSDVRTL
                     RFYAPQCPGERIFDFEITLFASHGPLTLGDTKEGTLAVRLAETLRLKGKIGQGHIINS
                     AGVRDGDTWGKRAAWCDYYGPVAGKTVGIAIFDHPKNPRHPTWWHVRDYGLFAANPFG
                     RHDFENLPDRSAGDLVVPPGKSVTFRYRFYLHEGDDQQANVAGRYKEYAR"
     gene            complement(9663..10562)
                     /locus_tag="PKI07_02575"
     CDS             complement(9663..10562)
                     /locus_tag="PKI07_02575"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC49966.1"
                     /translation="MTSTYRQETETSCVSSGPDKVLVPLPSAPASGTRKTCRRLAFTL
                     IELLVVIAIIAILAALLLPALAKAKGKAQQIYCLNNLKQLCLGWNMYATDANDFYPSN
                     STPSGFHAVENLGNWVTGWLDWGRGSPMGANTNKYYLINGSMGPYMGKSLGSYHCPAD
                     IYTCAYGVPRNRTVAMNSFIGDYNGTMVRFGNPNYLIFNKASQLTRPGPALTFVFIDE
                     CPDSINDGLFQMNMNNTMWSDIVAALHNNGGNLAFADGHAETHRWVDPVTRHGVVKGT
                     CPAYGKTSPRDYRWLQDRTTALK"
     gene            complement(10634..11122)
                     /locus_tag="PKI07_02580"
     CDS             complement(10634..11122)
                     /locus_tag="PKI07_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49967.1"
                     /translation="MIPESSERRHPRKRYREGLKCSVLDQLQSSGKSKAQVAQGIGIG
                     LGNLRDGKRQYGVAAKPVDALIPPNPVRELCRVPQLTRNGCYAWVRAGETARQAVRMS
                     RGGCGCDNAAMEAFGSTLKREAIAPSAAWLKDRVRRELIEYFEPSPTRPFLVFDASSR
                     LR"
     assembly_gap    11426..11525
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12225..12653
                     /locus_tag="PKI07_02585"
     CDS             12225..12653
                     /locus_tag="PKI07_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC49968.1"
                     /translation="MASVTQADALEKGPTRLVGACVLTDAAWRIVGHRLGLSGRELAI
                     VRGVFDDKIEMTMAADLGIAPRTVHSHLERLYHKLDINSRAQLVLRVMQESLTLMAAG
                     PPGGTPQICAGPGVGHLPLRLREQADVSAPSRGGKGSKRG"
     gene            complement(12743..13432)
                     /locus_tag="PKI07_02590"
     CDS             complement(12743..13432)
                     /locus_tag="PKI07_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOC49969.1"
                     /translation="MKKHINYLALVAAALFTLVRPAGAWPLKIYTLHDPIQDDWSISG
                     WAHELGNEPTFPEDERIESWEVPWGGWIPCPTDYKDGVVVQVAMRNLTRQTFYEGEVF
                     YVADADTSLSNWDEWVGQVDFDPGQAFMIDRRAALGGQWGQKNTPLVAESFIMDGIWQ
                     PGEIWQFVIQDYTSSWNGSPAYFGSLGIGGASGGGLSTGSIVIIPEPGTAMLLLLGGW
                     LLAFGTRRRSR"
     gene            complement(13728..14669)
                     /gene="gluQRS"
                     /locus_tag="PKI07_02595"
     CDS             complement(13728..14669)
                     /gene="gluQRS"
                     /locus_tag="PKI07_02595"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HOC49970.1"
                     /translation="MANPTPALPANPPAPLPPPAPYRGRLAPSPTGLLHLGHARTFWI
                     AQERARAHAGSLVLRSEDLDAARCRDEFVQAMLEDLKWFGFQWQEGPDCGGPFGPYAQ
                     SARQNLYRAAFNHLRAGGFIYPCTCSRKDILSAARAPHAEDEHEPIYPGTCRPLGASH
                     AQPAPNSPPATPSRPAGHRINWRFRVPDGEVIFFMDQNLGAQQFVAGRDFGDFVVWRH
                     AEVPSYQLACVVDDAAMRITEVVRGADLLLSTARQLLLYRALEWLPPRFYHCPLLTDS
                     TGARLAKRHDATSLRRLREAGQSPARLRAHWRVERAY"
     gene            complement(14809..15552)
                     /locus_tag="PKI07_02600"
     CDS             complement(14809..15552)
                     /locus_tag="PKI07_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOC49971.1"
                     /translation="MNLTLKVWRQKNAATPGRFQTYPARNLSPDMSFLEMLDVVNEDL
                     ITRGEEPIAFDSDCREGICGMCSIVINGIPHGGHHGTAACQLHLRHFQDGDTLTLEPW
                     RAKAFPLIKDLIVNRTALDRIIQAGGFVSVSTGGTPDANALPVPKDIADRAMDAAACI
                     GCGACVAVCKNASAMLFVAAKVSHLNLLPQGKAEKDRRVLNLVKQMDMEGFGGCTVTG
                     SCEAVCPKEISLSFIARMNRDYGAAIIKS"
     gene            complement(15549..17465)
                     /locus_tag="PKI07_02605"
     CDS             complement(15549..17465)
                     /locus_tag="PKI07_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOC49972.1"
                     /translation="MPLNAQIPSGPLAQKWEQYKFDLKLINPANKRKFDLLVVGSGLA
                     GASAAAALAELGYRVKCFCFQDSPRRAHSISAQGGINAAKNYANDGDSVYRLFYDTLK
                     GGDFRAREANVYRLAQVSGAIIDQCVAQGVPFAREYGGLLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALCRQIGLGTVKMFPRTEMLDLVVVDGHAQGIVVRDLVTGQISSHA
                     GDAVVLATGGYSNVFFLSTNAKGCNVTAIWRAYKKGAFFANPCYTQIHPTCIPVSGEH
                     QSKLTLMSESLRNDGRVWVPLNKADRAKPPAAIPETDRDYFLERKYPSYGNLAPRDIS
                     SRAAKEACDAGRGVGPGGLGVYLDFADAIQRLGQPVIRDRYGNLFDMYEKITGENASQ
                     APMRIYPAVHYTMGGTWVDYNLMSTLPGLFVIGEANFSDHGANRLGASALMQGLADGY
                     FVLPYTLGHYLATAKRPHPATSRAEFKQAEADTRAFIQRLLTIKGKRTPTSLHRELGR
                     LLWEKCGMARNETGLREALRRIPELRAEFWQNVNVPGESGDLNQSLEYAGRVADFMEF
                     AELLCHDALHRRESCGGHFREEFQTPDGEARRDDQNFAYVAAWEYQGLEKPPVLHKEP
                     LQYEEISMSTRSYA"
     gene            complement(17456..18181)
                     /locus_tag="PKI07_02610"
     CDS             complement(17456..18181)
                     /locus_tag="PKI07_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOC49973.1"
                     /translation="MNLITNLFQSSVGKKYIMAITGGCLLLFVLGHLAGNLQIFLGRE
                     IINRYAHFLQSNPELIWPVRMGMLVILALHIWAGITLSLENKAARPVAYAQYQPFGST
                     FASQTMLLSGLMVFAFLGYHLLHYTAKVQFINLTHQDFAAFTEKLPGPASVERPDIFK
                     MMVVGFRKPIVSAFYVLGLALLGLHLSHGASSLFHSLGWRTDAYRPCLDRAARVLALL
                     LFLGYCAIPAAILCGYGKEVLCP"
     gene            complement(18364..19722)
                     /locus_tag="PKI07_02615"
     CDS             complement(18364..19722)
                     /locus_tag="PKI07_02615"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOC49974.1"
                     /translation="MTYSDAIHFLYNLRWFGAKLGLENTFKLAALAGNPQHRLRFIHV
                     AGTNGKGSTCAMLESIYRAAGLRVGLFTSPHLVAFGERIQVNRHLISEADIARLATEL
                     QSLLRQGWPAASTAPRTANPAPKTDHPTFFEVITLMALCYFAERQCDLVIWETGLGGR
                     FDSTNIVTPLASVITNIQFDHQKWLGETLASIAAEKAGIIKPGIPVITAAEGTEPLRV
                     IEQTARRLQAPLTTLTPAHALHPPLDILSLPLSGRHQRLNAAVAVATVRALAAHFPVS
                     DELIRAGLSQVQWAGRLQLLTRPDGQKVLLDGAHNVGGAEILTTAVKEYFPSARITLV
                     LGILRDKDWPGMCEILAPLAARILPVPVPSERSAAPEELAALCRRINPHAQTSPHPSL
                     RSALAQTTPTDFVIIAGSLYLIGEALELLHFSPATATPERGLNEWDQPTAPPLPCLPT
                     AS"
     gene            complement(19740..20489)
                     /gene="kdsB"
                     /locus_tag="PKI07_02620"
     CDS             complement(19740..20489)
                     /gene="kdsB"
                     /locus_tag="PKI07_02620"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOC49975.1"
                     /translation="MKIIGIIPARWGSTRFPGKPLALIAGRPLLQHVVERCQQASALA
                     EIIVATDDERILKAVERFCQVEMTAPDHPSGSDRIAEVAARRAGDAIVNIQGDEPLIA
                     PSVINAVATALTQAEMSTAATPITNLADYDDPNTVKVVVNAAGRALYFSRRTIPYVRE
                     TAAASPREQLAAFPFLKHLGIYGYRRDTLLRLVSLPISPLERAEKLEQLRALENGIPI
                     AVVQVTHDSAGVDVPADVARVEHLLAGQTAG"
     gene            20730..22589
                     /locus_tag="PKI07_02625"
     CDS             20730..22589
                     /locus_tag="PKI07_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49976.1"
                     /translation="MHALQRLGFIGHVNQGRVARGLAPLNREEEEELSAESVDLVFDP
                     EHILIRPNPDRMDLAFAGDELLQTRVPKRQVKFLSVADRRVREAIKQRGECWRLSSIP
                     KTIEAKTRLVLGSKVGIHGQPIYFYNRLTGTRWLTCQEFAELGKLDDANLACHLREIA
                     VHSTLRNRLGHPELDFFAVDQQQFGAPQFAGTDYTQLGREQLRALFGDLCARFSAAVG
                     KDFCTDDPKNRAWSERILATLFLEGNETETEEILSGLSPEFYLQVEWLPGGRFEEGEF
                     LLDPIFDDAVRHPEDEELQRLCDSRAQGIIFNFIREYGDLDYINVGCVAESLSLGRPQ
                     KNSRRGVYLVELYSRSERAVIKRFLRLQKWSVWERLDEGKDLLQAIRESDDYTDYCLD
                     RRLGCHQLRMNLIRRVGLRRLSEIYRGSNARFYGEPIRTFYFEREYLSGIATDKLTPE
                     RYGRPGYGARLAELLGRAAASNLIVGRAQEAEEGKELCPLFDDGDELVREGDDGLPSE
                     ILVVDYTGAFVEYKLPLETFAARYAHPVNSRGEAIPDLRAFATAYLDALRDQFLQIQG
                     DYRIRRRAFDTLFQHCKYDPEGSFAYRWECVLSRLDQTNPYALVDAIRQHIEC"
     gene            complement(22740..23537)
                     /locus_tag="PKI07_02630"
     CDS             complement(22740..23537)
                     /locus_tag="PKI07_02630"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOC49977.1"
                     /translation="MIPEYTQMNPSQRCSPRRGLALATALLLGTSLISTQAQQASASS
                     APPSTNRWETTAAAGLTLTRGNSETLLATLSLDTKRKWEHDEVALGLAGGYGEDHKVR
                     NTEFVNAFGQYNRLLSERFYVGLRADFNYDGIANLSYRVTVSPLAGYYLIKEARTSLA
                     FEAGPSVVFEKYQNQSSDTYAGLRLAERFEHKLTDTTKIWQSATYVPQLDRWTEKYLL
                     TFEAGIDTAITKKWSLRVVFQDIYDSEPAAGNDHNDLRLIAGTAYKF"
     gene            complement(23593..25452)
                     /gene="glmS"
                     /locus_tag="PKI07_02635"
     CDS             complement(23593..25452)
                     /gene="glmS"
                     /locus_tag="PKI07_02635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOC49978.1"
                     /translation="MCGIVGYVGRKEATPLLIEGLRRLEYRGYDSAGICVLTNGTLHV
                     RKQVGRVEGLAQSLKTKPVKGSLGISHTRWATHGLPSDINSHPHLDQSGKVALVHNGI
                     IENYTTLRHRLQRLGHRFLSQTDTEVLAHLIGHHLDQALARKGEVTPERVTQAIFAAT
                     REVEGTYAIALMHQGLPGHLFGARRGSPLVVGLGQDENFLASDVSPIVAHTRKVVYLH
                     DGDIVTLTNKDFALQARGKAIQRPISSVDWSAEEAERGKHPHYMLKEIFEQPGRVAEV
                     LRRTLRPAKGNVEFEEMALKPKELAKINRLILVGCGTSWHAALVGEYVIETLARLPVE
                     VEYGSEMRYRGRPIEKNTLIVSISQSGETADTLAAIRAAKAQGARTLGIVNVVGSTIA
                     RESDADIYMRAGPEIGVASTKAFEMQVLQLTLLGIALARQRGELSPAAGRKLVRELQT
                     IPGKIEKILAGDAHLRKISKKYHQATGFLYMGRQFNFPVALEGALKLKEISYIHAEGY
                     PSAEMKHGPIALIAPDFPSVFVVPRDAMYDKNMSNVEEIRARKGPIIAVATEGDAEIK
                     HKASDVIYIPQTLDILQPLLTVVPLQLLAYHIAVLRGCDVDKPRNLAKSVTVE"
     gene            complement(25515..26060)
                     /locus_tag="PKI07_02640"
     CDS             complement(25515..26060)
                     /locus_tag="PKI07_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC49979.1"
                     /translation="MNRAVFLDRDGTIIAEKNYLSRVEEVEIFPGAGAALGLLCQAGF
                     RLFLVTNQSGVGRGYFTLAQVHEIHQYLLRELDRDRVRFEKIYIAPEAPGAPSRGRKP
                     SPQFLWDARAEFGVDLAQSYMIGDKLIDLECGWNAGVKQCLLVRTGYGAELEQEAADK
                     LAAAVVVDDLPAAAQWILKQT"
     gene            complement(26206..27810)
                     /locus_tag="PKI07_02645"
     CDS             complement(26206..27810)
                     /locus_tag="PKI07_02645"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HOC49980.1"
                     /translation="MPGPSARFINRNWPFTLWQLLILLFLGGISCASVHACTLWGAAG
                     VDAGGGTIISKNRDWKPDHVQVLKMQRPTNGYAYFGLYAEGNDAPGIKEGINEKGLTV
                     VTASAGTIPKQTREAQPGKGSLISTLLANCANCDEVLAQQDKLFSQRRPTFVMIADRK
                     QILMLEVGLQGRYSMRAVKTGTVVHANHYLDETMQKFNLKTNRSSCVRVKRIADLLQA
                     APRPFDTAQFASMSRDRHGGPNNSLWRTGTNGCTLSSWIVETPAQGAPRLRVLIANPN
                     QPEELRQYVLDNTFWKKPAGTAAIQAATCEGAYERHLQGICTNERDALYWSWTDALVK
                     TDRHGRILKRLEVASHHGDLCHHLGEVFVAVNLGAFNRPAGYANSWVFVYDADTLIER
                     ARHPVPEVEHGAGGIAYHKGRFIVVGGLPEGVNENYLYEYDEKFAFRKRHVLASGYTL
                     MGIQTVAFAEGAWWFGCYGTPKVLLRANPRLQLTDRWEFDASLGIVGLGNQRFLIGQN
                     NKRPNVGYEGRAAIARFTAEKGLIFE"
     gene            complement(27863..30424)
                     /locus_tag="PKI07_02650"
     CDS             complement(27863..30424)
                     /locus_tag="PKI07_02650"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOC49981.1"
                     /translation="MPRRKFLQLSAATVGAVGGLGTVPPAQAVPSRSGRRDARPTGRR
                     TGRVYNGGYSGEYLNQVAFPLGGIGAGMICLEGTGMLSHFSLRNRPDMFNSPCVFAAI
                     SVKGQPRAARVLEGPIPGRKLYGPQGAGNGLGGTSYGLPRFAQASFKARFPFGTVTLT
                     DPQLPLSVAITGWSPFEPGDADNSSLPVAGLEYQFTNRSGKPLEAVFSFNARNFMVTG
                     GNPQAVRPIAGGFILWGGGSTDHPWDEAAFSATVSEADTKVNHAWFRGGWWDALTLAW
                     KDITEGACFERPPVTTGRPSPGGTLFVPFQLAAGAAKMITVRLAWFVGQTNLRIGRDP
                     AGLKPEAVSRDHYRPWYAGRFTDINGVSFYWRDHYDELRQKASRFADCFYASTLPLEV
                     LEAVAANLTILKSPTVMRQTDGRLWGWEGCHDNAGCCHGSCTHVWNYAQAIPHLFPRL
                     ERTLRETEFGPSQDEAGHQQFRSALPIRPVEHDFHAAADGQLGGIMKVFREWRISGDT
                     EWLRGLWPKVKRSLEFCIKTWDPGHKGVIEEPHHNTYDIEFWGPDGMCTSFYLGALQA
                     AVLMGKALGDEVPLYAQLLAAGIRRTESELFDGEYFIQRIDWTNLKAGNPLENKNLTG
                     NFSPEARAVFEKEGPNYQYGTGCLSDGVLGSWLALVCGVGQVLAAPKVASHLQAVHKY
                     NLKHDLSDFANPQRPAYACGAEGGLVLCTWPKGGELSLPFVYSNEVWTGIEYQVASHL
                     MRMGLVSEGLEVVRVCRDRYDGRVRNPFNEYECGHWYARAMSSYALLFGLSGARYDAV
                     EKVLYLEPRLRGDFRCFLATASGYGLVGVRKGRPFYEPRAGTLDIREIKYVKYTG"
     gene            complement(30650..31186)
                     /locus_tag="PKI07_02655"
     CDS             complement(30650..31186)
                     /locus_tag="PKI07_02655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOC49982.1"
                     /translation="MLKNLCQKMFGRSLWVYHANSGGCNGCDIEVLNVLTPYYDVERF
                     GIKLVGSPRHADVMLCQGPAMRSTAKALQRAYEAMPAPKLVFAIGSCACGGGIWFDSY
                     SVLGPVEKLVPVNFYIPGCPPRPEAIIYGVAVAVGLVDKKVAPVTFKQLEMPIPRYHP
                     ADLRGSGELIVYEKAEKV"
     gene            complement(31369..31893)
                     /locus_tag="PKI07_02660"
     CDS             complement(31369..31893)
                     /locus_tag="PKI07_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC49983.1"
                     /translation="MSLLSKLQETLVCLKAGRVTLPYPAQAGPVPETFRGRPIFDAAR
                     CIGCAGCASNCPAREILVVDVCQEIRILQYLGRRCTYCGRCADVCPEKAITMSREFET
                     ATDRIGDIRQRLELFMSTCQRCGRCFTEPSPLEQLKLKGYRFDDVQNERWIFRSQAYL
                     TSEAAVDDISIDLD"
     gene            complement(31903..32823)
                     /locus_tag="PKI07_02665"
     CDS             complement(31903..32823)
                     /locus_tag="PKI07_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOC49984.1"
                     /translation="MSPVTVILGFLVNVVLVLLLAPLFQGVLRKVTAQIQSRQGPPLP
                     QPYFDLLKLLGKEDLESGQMPAMQRFAACLSLATMLTLAGLVPMGFAAPLNGAGDVIL
                     VIYLLTLSGICTLLAGLAAGSTYSLVGISREMMTMIALEPLFAVAIIAGAIHTGSFRL
                     DTVFNGSVYAGNTFPWSGLILLGVILLSFQAFVQRVPFDLSEAETELMEGPLMEYSGP
                     KLALFKYAQMARLVIYSALFVNLFVPWGSALAFPLGWLLFWLKVCGLVLLVTLVAATH
                     ARYRIDQALRFFAALLGVGLGALILACYGY"
     gene            complement(32825..34495)
                     /locus_tag="PKI07_02670"
     CDS             complement(32825..34495)
                     /locus_tag="PKI07_02670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOC49985.1"
                     /translation="MTARERLNQLKEKFGPAIQRADLPGDSRLFAYVEPATLKAVCQY
                     LFRELDARYVISIGLDDRPFSGKFLVAHNFAFDRDRLLCSMLAHLPADHPEVDSIAPA
                     VPGANWAEREFRELVGIELRGHPYPKRLVLPDGWPDGVHPLRKDVPWNQVPADFDENA
                     EFTFDDPPAGCTVVPFGPFHPTLDEPEHFRLYVEGEMVRGCEYRGFMVHRGIEKLAES
                     VLTYNDIPLMAERICGICGCVHNVTYAQAVETAVALRPPPRAEYIRTIMLEIERLHSH
                     LLWVGLACHIVGFDTLFMQSWRIREPIMWIAEKISGNRKTYALCVIGGVRWDITPEVK
                     VELRHLLDKLEREWREVVAAVSRDKNIQKRTRGVGVADQALIKSLGLIGPVARAAGVD
                     IDCRRDHPYAAYDRVDFAVITDTGGDVWSRVVVRMNEVFESIRIIRQCLEKMPEGPLQ
                     ASIPDELPPGRLGLSSVEAPRGESHHFVITGENNRPHRWRVRAPTYQNLQAVPMMIKD
                     QQLADMTISLGSIDPCFSCTDRLETIDLKSGATQVWSQAELIRLVRKT"
     gene            complement(34492..36411)
                     /locus_tag="PKI07_02675"
     CDS             complement(34492..36411)
                     /locus_tag="PKI07_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOC49986.1"
                     /translation="MTPEQSVLAAIVLCLGGAGLTLLASRNKTVAGGLAFVFTLAAAV
                     IMVSTVTRVLISGPGHSAEFSLLPQIGLVLRVYVDGLTALFLLLTALVSVPAALYSVV
                     YMRNYEGYSAARYYPYFLIFLAAMYGLVSTTDMMWFFFIFWQMMTLPGYALIRFEHRK
                     PANIRAANRYLVMMQIACAATMIGAELLAVAGATTSGSPGLKYDFDTVSANLPALLSA
                     RPGMTALAFALFLAGFGIKMGMWPFGQFWLPDAHPAAPSPVSAMLSGVMIKTGVYGLM
                     RYFLWLVPPAGLADYPLAPWGLVVALLGTITLFTGTMQALKQEQSKRLLAFHSIGQVG
                     YILLGTGVCMRSLLPAADPRAAALAALGFCGALLHVLNHGLFKALLFLNAGSMLQATG
                     TQDLNRMGGLMKFMPLTAITALVASCSISGVPLFNGFISKWTIYVAAVQGCGSAKYLA
                     VCALLAILTSALTLASFIKFFGVSFLSRASALVPARAARPGRLEVGWTMQLPQVALAL
                     ACVLLGVAPALGFRLVQRVLETSSSGCGTALAAAAPLQTSLWSGVAQLQSAALFAPLV
                     LAAALGLLLLAAHALSRIGHASRRATAPWLCGYAREADCHRYSAHNFYAEIKRYFRWL
                     GGAPDNRNLHPEEKP"
     gene            complement(36408..36962)
                     /locus_tag="PKI07_02680"
     CDS             complement(36408..36962)
                     /locus_tag="PKI07_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOC49987.1"
                     /translation="MPDLRQLLAPLLQGRVCLVGIGNVDYGDDGFGVRLAEALQASPI
                     TAEVMIAGSAPERCLGRVANGGFERVIFLDAVDFGGAPGAVILLDARELIARFPQVST
                     HKLSLGLLARQIEAQGRIRVWLLGVQPESLKLRGALTPTIQTTLELLHALLTAGAENC
                     RLARSESPEPGSKPAETTLREVLA"
     gene            complement(36962..37837)
                     /locus_tag="PKI07_02685"
     CDS             complement(36962..37837)
                     /locus_tag="PKI07_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOC49988.1"
                     /translation="MNPTLPKVTSCEPAEENIYLPKLAVLDRVTDDIPEVKTFYWHFE
                     DPAEQAKFKRFRPGQFAQVSLFGVGEFPASLPPSPTEEETFFTIRTVGSCTAALHQLR
                     PGDRFAVRGPYGNGFSMEDYYGKNLVFVAGGIGLIPLRSCIVYALRHRDKYQRIQIFY
                     GARTPKELMYVPNLREWQQSAGVECHLTVDRAAEGWDGPVGVVGSLFQRPGVKIPVEN
                     TIAFVCGPPIMFRFVIKDLLAMGFQEKDIVSTLERYMKCGVGKCGHCCIGVAYVCVDG
                     PVFTYEQIKKLGEDI"
     gene            complement(37834..38916)
                     /locus_tag="PKI07_02690"
     CDS             complement(37834..38916)
                     /locus_tag="PKI07_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC49989.1"
                     /translation="MKARLLERAQVLTLVDLLRQQGYDVFAPFAGRGRDTWFDLVTDE
                     NRGQVQIHLPNPYYPPKRFVLPHIERLLKVRRHDGTFHIEPIYRERKRAIFGLRSCDL
                     AGIWHLDRFYLGREFRDLYYQKRRENLLLINVVCTDLERDIGDECFCVCADTGPAARD
                     HFDLQLMDLGDEFMVVAGTAAGEAFFTQPMFRPATAAHVEKRRAILAEVRKRFKTTTS
                     WFAATVRYVSRGDILEQTWEEIGNRCLECGGCTYVCPACTCFTVSDRQVGPDEIERVR
                     IWDACALSGFTRMAGGFNPRRAVHDRRNRRFMRKLAHYFIQRELTMACVGCGRCAAVC
                     HGDVGMPSVVEMIRRATAIHDRIPPS"
     gene            complement(38913..40940)
                     /locus_tag="PKI07_02695"
     CDS             complement(38913..40940)
                     /locus_tag="PKI07_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOC49990.1"
                     /translation="MAHKLTTCTFCGVGCGIYLEAVGNQIVGAYPSMSHPANEGRICV
                     RGWHVHEVASSPGRLKRPLLKKGGQFEEVSWEEALGFIIGRMKELHARHGAEAFAFLN
                     SPRCSNEESYLLQKLARAVIGTNNVDHGTGVYCNNSINVLLDMLGVPATTNSIGELAR
                     SEVIIVDGVDLALQLPSIGGTVIRAKLAGATLVVIDSRRHRVAESADLFLQLKPGTEP
                     ILYGALAKVIVDRGLMNLPFIKAHCRDYDRFLARVRDYDLLAAAEACGVDAGLIEAAA
                     LAYARARSAAILYSTGIEARDAEPVQAIVNLALLTGQIGREGAGIFALTEHNNLQGVC
                     DMGMLPDRLPGYRQVDSAAARAEVGRVWQTKLPAAPGLASRAIFNNRGASPVRAIWLC
                     RYDPVSSAFFGDAADALDRCELVVAQHLFLTDTARHAHVVLPTTAFGEEQVTFTNTER
                     RIQLAEQIIDPLPGTTPAWRQLTQVARALGADWRYESAADVMDEIGAVIPFYGGANYR
                     NLAAEYGRQWPCSKDRPLGTRYLFADGLPAQGFKFAPIARHAPAAVVSDEYPLTLVFG
                     HSLYYWNQSVLIKHSETLKREYRILLLDYPEGFVEINPADAQQAGIRDGEKVRLTAAG
                     GSAVINARVTSEVRQGAVYVPYFVRQVQRQICGAAQSGVQLIPARIAKEPA"
     gene            complement(40991..41353)
                     /locus_tag="PKI07_02700"
     CDS             complement(40991..41353)
                     /locus_tag="PKI07_02700"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOC49991.1"
                     /translation="MLVHLLKSKIHRARVTAGDVNYEGSLGIARDLMDKVGLLPYERI
                     LCGNMANGERFETYAIAAEPGSGQIILNGATARLGKPGDLITILSFTEVEWAAAQTWK
                     PRVIVLGEDNRIIRERGI"
     gene            complement(41476..41715)
                     /locus_tag="PKI07_02705"
     CDS             complement(41476..41715)
                     /locus_tag="PKI07_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC49992.1"
                     /translation="MMPLYEYELCEGDCVVCGGKFTLRRPLSAAPLTQCPACKKPVRR
                     VWSTFNSPRTVSISDAKKAGFTVLKRVGKGEYERQ"
BASE COUNT         8334 a        12334 c        13194 g         8187 t
ORIGIN      
        1 aagatcatgc ttaccatagt tcagatgtcg gaaaaagcaa tctgaatcca cgtcaaactg
       61 gccaagtagg ttgctttaaa aacagggcag gaagtgaggg ttaaactaaa taaacgcttg
      121 atgccttttt gtcaccaaac cacaggtttt ggggtgcgtc ttgagcagct tcgccgacgt
      181 tgttcaggtt gggagcggcg ataaaaggac tggcgatagc gccgcctgca ggctagtttg
      241 agggctaata tgaacttcgg gggccgcaaa tggattgccg aatggctggt cggcttgggg
      301 cggatcacac ttctggcgcg ggaagcagtg ggttcgcttt tccgcttcaa ggtggcgtgg
      361 cgtgacttcc tttatcaagt ccactttgtt ggcgtcaaat cccagtcagt ggttctgatc
      421 accggcgcct tcaccggcat ggtcttggga gcgcagacct atttccagtt ccataaagtc
      481 aaaatggata ccgccatgct tgcggtggtc agcgtttcca tgtgcagtga attggggccg
      541 gttctgaccg ggctcatggt tgccggtcgc gttggcgcgg ccatcgcagc cgaactgggc
      601 accatgcgcg ttactgagca gattgacgcg cttcgcaccc tggcgacgca cccagtggat
      661 tacctcgtcg tgccacgcct gctggctttg cacctggcgc tgccgttgtt gacggtcgaa
      721 gccatcgcca tcggcatcgg ggccggctat ttggtggggg tgtttctgct gggggtagat
      781 ccagtttact cgtggtacaa tatgctgcgc tacacaagca ctggcgatgt catcgtcgga
      841 ctgatcaaag cggtgttttt cggcggcatg attgcgttgg tggggtgcta caaagggctt
      901 tactgcggcg agggagccga gggcgtgggg cgcgccacga ccgaagcggt agtatattcc
      961 tcgattgcca ttctgatttc caactttttc ctcactctca ccctcgggcg cctgctcaac
     1021 acgttatgat cgaggttcgc aatctgacca agcacttcgg ctcgaaccct gtcttgaagg
     1081 atgtgagctt ccgcatcgag aaaggtgaat cggtggtcat catcggccgg agcggttgcg
     1141 gcaaaagcgt gctgctcaaa catctgatcg gactgcttca accggacgct ggccgggtat
     1201 tgatcgaaag cgaggatatt gtcggcatgg gcgaacgtga attgctgcgc gtccgtcgca
     1261 aatttggcat gttgtttcag agcgccgccc ttttcgattc catgactgtc gcggagaatg
     1321 ttggctttgc ttttcgccgt gagcgtgccc tgcccagccg cgagattcgc caaagagtcg
     1381 ccgaggtgct cgaaatggtg gacttgccag gcatcgaaaa caagaaacct tccgaactgt
     1441 ccggaggcat gcgaaaacgg gttggtctgg ctcgcgccat catctatcgc ccggctattg
     1501 ttctctacga cgagcctact accggcctgg acccaattgt atcagacagc attgaccaac
     1561 tcatcctgcg ggtgcgtgac cgattgtcgg tgaccactgt cgtggtcacg cacgacatgc
     1621 gcagcactcg ccgcctgggg cagcgcattc tgatgctcca caaccggcaa atctacgccg
     1681 ccgggaccgc cgacgagatc tttcactctt ctgatcccat cgtaaggcgg tttatcgaag
     1741 gagtttccga tcccaaagag ctctactttt gatatggata aatcgcgtct cgaatggaaa
     1801 gtcggcctgt tcgtttttgt cggcttggtg ctggcggctg gcttgctgct ccagttcagc
     1861 aaaggaacaa ccttcttccg gcccgcttat gaaatcatcc tcgaagcccc tactgtgagc
     1921 ggtttaaaac cccgcgccca agtgctgatg tcaggcattc aggtagggtc tgtgtccgcc
     1981 atgcagcttg cctcagatgg caaaagtatt ctcattaccc tccgcatcta ccagcaatac
     2041 caaatctaca aggatgcccg ctttgtcttc gagcaatccg gctttctggg ggatcaatac
     2101 gttgctatcc ttcccaccca aaatcaggga gaagtttttc gtcccgggga tcgtgctgct
     2161 gccgaggcgc cgctggatat acagaaaata gcccgctccg ccgccggatt gctcgaacac
     2221 attgacgccg ctgcgactaa catcaatcac gttctgagcg acgcccgcct gacggtgctt
     2281 agtgcgcgcg ctttgaccaa ccttcagacc gcgttcgaca attttcaccg cgtttccgag
     2341 cggtctctcg aggtcgtgga aaatgccggc gccctgctgg acactaatcg cccaaccgtc
     2401 tccgccgcgc tcagcaattt gctcagcttc tcggatcagg ctaatgaggc ggctggcgac
     2461 gtgcgcgccc tgctggctgc caattcatcc gccatcacta cttccctcag caatattcaa
     2521 tcttccactg ataccctgaa agcactcctc gataacctca agcagggcag gggaacggtg
     2581 gggaaaatgc ttgaaagtga tgaaatagcc gccaatgttt cgcaggttgt gagcaatttg
     2641 agtatcacca ccagcaacct caaccgcttg ggcctctggg cagtcttgcg caagcctcgg
     2701 cccgccccga ctaaccctcc cgcgcgtgcc gtgctgaccg ctcctaaaaa tccttttcct
     2761 tgaataaaac ctatggcgct ttgggcaatt cgtatcctgt tcctgggtct ttgcactggc
     2821 ggtggctacg ctattagcca ggttcgttcc gaatggcttg gccacggcgc ctggttcctc
     2881 atcgccggtt tcggcttcgg ctggctgctg attgcgattg acgaaatgct caagggcttt
     2941 tcgctgcgcg cattttccgc caccaccttc ggcctgttgc tcggcaccct cgtcgccctg
     3001 ctcattgacc actcgggcct ttttggcgaa cccaacgata attctcccca ccgcctcatc
     3061 gtccgtctgg ccctttttgt cggatttggc tacatcggca tggttctggc gatgcgcagc
     3121 aacaaggaag atttctccct gatcattccc tatattcgtt tcgcgccgca gaacaagcct
     3181 gacaacctct tgttgctcga tactagtgcc atcgtggacg gccgcattgc ggacctcatc
     3241 gaagccaact ttattgaggg cctcatcgtc gtcccgcgct tcgtcctcaa ggaactccag
     3301 caactggccg attccaacga ccctctcaaa cgcgcccgag gccggcgcgg cctggacatc
     3361 ctcaaccgca tccagcgcaa tacccgcaac gaagttaaaa tccacgacgg tgatttcacc
     3421 gacgaagccg cggtggacgc caaacttgtc cgcttagccc gcaacctccg cgccaagctc
     3481 tacactaacg actcgaacct ggctaaaatc gctgaacttc aaagcgttaa ttgtgtcaac
     3541 cttcacgaga tagccaaagt cctccgcacc gtgcttctgc ccggcgaact gctcaacttg
     3601 cgcattgttc gcgaaggcaa ggacaaaggc cagggcattg gctaccttcc cgacggcacc
     3661 atggtcgtcg tcaacaacgc ccaggaagcc attggacagc aggttcaggc tcttgtccaa
     3721 agccagctcc aaaccggcgc cggagtcatc gtatttgccg atctcaagcc gcctgccact
     3781 acttaagccg ccggcctcgg ccgcgcccct gttttccctt cgccatgtca cttctccccc
     3841 tgacctttgg ctcagcaggg tgggttggct ttcttcccat acccagcaac tcccctatca
     3901 gcacccggat gcagccgagc ctccgttgtc taccctgact aattgccgaa tttcgaaggt
     3961 agctcgaggg cacctgccct ccttttacct gttttatgct gcggttgctc agcaaggtaa
     4021 agcctctcct ccgattacgg ctggcacttg gggggcagac cgatggcgca ccgtttagat
     4081 gcaggaggcc gtcgttttcc caacgctcat ccatccagtg gccgttccct gcctgttaag
     4141 cggccactca ctcctgctat taccccaaac tgagaaacgt ctgcccgcaa ccgactgttt
     4201 agttgaaagg gttactgccc ggcagtattt gatttgagct tcgggctttt ggatgtaaac
     4261 cggtccccat tcatggtgcg caccttccag ccgaatggac ggagttcgag ggttgccgct
     4321 ccggtgttgc gcgtataaat aaccggcacc ttccagcatg ccagccgttc ggtcagtttt
     4381 gcggttgctc gttccgcggc gggaaactca gaatcagcaa cgataatcag gcgaggttgg
     4441 atagcttcca gcagcgcgtc gctcagcgct tcatactggc tgggcaatcc tgtgactaca
     4501 atatccgcgc gcaaatcggg cgttcgttcg agcaacgcgt tctgaccggt gcggcctaaa
     4561 tcggaaagca ataatacccg cgtgccatga aaattgccca acaaaaccag cgcgttgtcg
     4621 tcggcctgtc caaaccgatc gtcagcttcg gggtgcagta ccttccaggg accgaagcgg
     4681 tcgccgctgc tgatccttcg cagccattgc ggcttgcgct cgaattcctg caggagcagc
     4741 cgatatttgg gtgaacgaaa gcgcaccggg ctaaccagaa tttggcgtgc gtgaaacaag
     4801 tccgcgatta catccgcgcc gccaatgtgg cgcaaatcac cgtgcgtcag gatcagcgct
     4861 ggcagggagt tgattccctg ggcgcgcaga aagggtttgg tgacgaactc aacggaattc
     4921 gttgagccgc agtccatcaa ccaacggtca gactggtttg aggcgtggta gtaaagagcc
     4981 atcccgccgt tcaccgggat cactgtcagg ctcgcgggcg gccgcattgt cagccattgc
     5041 caggcataaa agcccgctaa aaccgcgacc cccatcagcg tccagcgtcg cggcttcgcg
     5101 ggcaggaaca aacctgccgt cccgccgatc aagataaggt agccggcaat cactccccac
     5161 cccgccggcc cccggacgta atagtaggcc ccgggaagct gcgtggccca ctcgctcaat
     5221 ttgaccatgc aactcatcca gagccagccg ctgtggttga agcattcccc gacccacggg
     5281 agccagcttc cgcaaatcag gctgccgaca atagccgcca gcgtcaggct gcttagtggc
     5341 acaataacca aattggccag gaccgtcacc gggctgaaga gatgaaaata gtacgccgtc
     5401 aggggcagcg aacccatcca ggccgccagg gataccaccg ctgcggcggt ccctccccgc
     5461 aacagaaatt ccaacccgcg gcgccagcgc ggcaacaggt ctgccggtac cagtgggtca
     5521 gattggagca ggcggttgcg catggcctcc agcggcggcg cgagcagcgc aatgctcagc
     5581 acgaccagaa acgacaattg aaagctcgcc gtgaatattt gccggggatc ccataacagg
     5641 ataagaaggg cggccgccgc cagggagttc aacaaatcac ttggccgctt cagagcccag
     5701 ccgccgatga taatgctcat catcaccgtt gcccgaatgg cagatgcctg ccaacccgtt
     5761 gccgccgtgt aaaaccagat caatgggatc accacccagc cgcatcccac gcggggcact
     5821 tgcagcaggc gcagtaagcg cgtgagaata cccgcgatca gggccacgtg cagaccgctg
     5881 atggcgaaga tatgcattgt gcccgagcgc atgaagggtt cataggtttc gtgggtcaaa
     5941 gcgggtttcc agcccagcga catggcccaa atcaagcgca ggggctcatc ttccaccggc
     6001 aggccgcgag ccagggaggc tcgggcccag gcacgaaacc ggtcgcccaa cggtcgaggg
     6061 cgccggccgt ccaggacttg ccagtcattg gttgtggacg cggtgaggag gtaatggatg
     6121 ccgtgccgcc gcaggtaagt gcagtagtcg aataagccct cggccacggg gcgcggcggc
     6181 ggcgccagaa cccccgtgat ctggacaacg tgccgggcat aaatctcatc gggcagccgc
     6241 gtcggtgtgg tgaccaatac atcgccaaaa gcaggttgcc aggcgtcgtt gccgcattgc
     6301 aggtggctta cccggacttg cgcgattgtc cgcgtggttt ccttttcatt gcgtacgtaa
     6361 gttcggtgcc cgggcgtctc cgtaagtgtg ccccggatcg ttgcgagtat cggctcggag
     6421 ccgacgatca ggcgcaagtc atggggagag agcagggcgg tgcggaaagt cagattggtc
     6481 caaccggtga agagcaccag cgcccaaatg agccagggcc gcgccgcggg caaagccagc
     6541 gcgcccaccc ccagccccag ggagatggcg aagagaggaa cgagcggggg ttggtaaagc
     6601 tccgcgagca gcagcccgcc aatataaacc agggcgatcc aaaccaaggg gcgtttcatc
     6661 gcgagcgcga gcttatgcgg acggcggaag catgcactgg gtcgggacga agaaagcggt
     6721 gcgggtcagg aaagaaactt gccagggttg aggatgccgt gagggtcgag ggtttgcttg
     6781 agggtttggt ggagggcgcg gacgttggga ggcagagcgc ggggccacca acggagcttg
     6841 gccaggccga tgccgtgctc gccggtgatg gcgccgcccc aggcgatgat ctggcggaag
     6901 agatcgtcaa gggcaatccg ggcgcggttt cgggcgccag gctgttgaaa atccaccatc
     6961 aggttggtgt ggatgtttcc atcaccggcg tggccgaagc aggcaacggc caggcggtgc
     7021 cggcgctgaa gacgggcggc gaaggcgaaa aggtcttcga ggcggccccg cggcacgaca
     7081 atgtcttcgt tgagtttggt caggccggta tcgcggaggg cgtaagagaa ctcgcggcgg
     7141 atttgccaga gggcttcaca ctgggcggga ccgaaggcgg tttggatgaa aaggggccgg
     7201 cgcggggcca ggagtgctcg cagctggcga atttcgccgc ggacggagcg cgtttgtccg
     7261 tcgagttcaa caatgaggtg ggcgcggcaa ccgcgcaggc gcgggctgcg cgtgcggcgg
     7321 taggcggcag cgagcgtgaa ggagtccgcc aactcgaggg cggcgggcag aaagccggca
     7381 gccagaatgg cgtgcaaaga gcggacagcg gcacgcatgg aggcgaagcc gagagcaagg
     7441 caggcgcggt agggaggcag gggcagcagt ttcaaagtag cttccgtgac aacgcccagg
     7501 agaccctcgg aaccgacaaa aagacgatgg agatcaaagc cggttttgtt tttatgcgtg
     7561 cgtccgccca ggcgcaggat gtcgccgccg ggcaggacaa cttccaagcc gaggacgtag
     7621 tcgcgggtga ccccgtattt gaggcagcgg ggaccgccgg cattggtgag aatgttgcca
     7681 ccgatggagt tgtcggcgcg gctggcgggg tcggggggat aaaagaggcc ttgcttttcg
     7741 acggcttgct ggaggcgggc ggtattaact ccagcctgga caacagcaac gaaatcggcg
     7801 gcgttgattt cacggatgcg attcatgcgg gcgagggaca aaacgatgcc cccgcgggag
     7861 ggaacgcagc cgccgacgta gccgtggccg gcgcctcgcg ccgtgacagg gatgcgatgc
     7921 cggtgagcaa aacgaaggac cgtggcgacg gaacgggtgc tgcgggccag gacaacggcg
     7981 tcgggttggt gggtggcaaa ccacttgtcg ccggcgtgtt gggttaggac cgacgggtca
     8041 aagctgattt cgcccggaga caggaggcgt tggaggcgac ggagcaattt aatctttttc
     8101 gggttcatgg tcagacgggg aggcggtcag aggccggcgc ggtttaccgg gcatattcct
     8161 tataccggcc ggcaacgttg gcctgctggt catcgccttc atggaggtag aaacggtaac
     8221 gaaaagtgac gctctttccc gggggaacga cgagatcacc ggcggagcgg tcgggcagat
     8281 tctcgaaatc atggcggcca aagggattgg cggcgaagag gccgtaatca cggacgtgcc
     8341 accacgtcgg gtgccgcggg tttttagggt gatcgaagat ggcgatgcca acagttttac
     8401 ccgccaccgg cccatagtaa tcgcaccaag cagcgcgttt gccccaggtg tcgccatcgc
     8461 gcaccccggc gctattgatg atatgaccct ggccaatctt gcctttgagc cgcagggttt
     8521 cggccagacg caccgccagg gtcccctcct tggtatcgcc cagcgtcaat ggcccgtggg
     8581 aggcaaacag cgtaatctcg aaatcaaata tccgttcacc gggacactgc ggcgcataga
     8641 agcgaagggt gcgcacgtcg gaacaaaccc tcctgccatc ggcggcgacc cacaggttgc
     8701 gcgaacgaat gagaccttgc cggcgcccgg atttgatctg caggaaatcg tcatgcaccg
     8761 tttttccaaa ccccttctgc tcggaccaaa aatcgtgccc attgaccgcc ccgtgggcga
     8821 accacagcga gcgatgatgc gggtgatcgt gagcctcgcc gggagtatcc ttcatcggcc
     8881 aatcgcgggt cagggcggcg cctcccgggc cgatgagcgg gtagcaatag gggcggggca
     8941 catctttgaa gaaatactcg gtgaaaagct ggccgtgcat ctgcacgcgc acccggtcag
     9001 ccagcttcgt aatttgcacg cccggtttgg cggcggcggc ttttaccttt gtcgggccag
     9061 cagccggcgc cagaataagc aaggcgaggg cagcgccgaa ttgcgagatg cgtaatccaa
     9121 acatgatggg cgtatattgc gaagcgtcgt agctacggtc aaccccgtta ggagcgccct
     9181 atgagggaaa gacaggccgg caaccagggg aataataaaa gccagctccc tgcagccctc
     9241 aatgccccgg ttgcccgcgc cttgtcaggt aatagtcacc cgcataggac gttgcggcta
     9301 aagtcatctt ttcaggaaaa tctctgcatc cgccgataag tcctgcaaac caagccccag
     9361 accgcgcttt agagggttct tctacgattg gagtggcgga accgtcaata ccctccgttt
     9421 tttatgaata cgagctgagg atcgctactt tcggttctca gggcatcgct cgccacgaag
     9481 cgcccaggct tgacctgcgg caggaccgga atttccctat cgcgcgcagt cacgacgcac
     9541 gcaccaaaag cggccacctg tcaattttac actcggagcc gcagcccgaa catatccgac
     9601 gcgcgcaagc gcagcgaccg aggttggcgt ggcgaacctc gtcggagaga gcgtgggcag
     9661 gcctatttca aggctgtggt gcgatcctgg agccagcggt aatcccgggg ggacgttttg
     9721 ccgtaggctg ggcaggtgcc tttgacaacg ccgtgccgag tgaccgggtc aacccatcta
     9781 tgggtttcgg cgtggccatc agcaaacgca aggttgccgc cgttgttgtg gagggcggcg
     9841 acgatatcgc tccacatggt gttgttcata ttcatctgga acaaaccatc gttgatggag
     9901 tcgggacatt catcaatgaa gacaaatgtc aaggcgggac ccgggcgggt aagttgggag
     9961 gccttattga aaatgaggta attagggttg ccgaagcgga ccattgtgcc gttgtaatcg
    10021 ccgatgaaac tgttcatggc gacggtgcgg ttgcgcggga cgccgtaggc gcaagtgtaa
    10081 atgtcagccg gacaatgata gctcccgagt gacttgccca tgtaaggtcc catagacccg
    10141 tttatcaggt aatatttgtt cgtgttggcc cccatcgggc tgccccgccc ccaatcaagc
    10201 cagccggtga cccagttccc caggttctct accgcatgga accctgatgg agtggagttg
    10261 ctgggataga agtcattggc atctgtggcg tacatgttcc agcccaggca gagttgcttg
    10321 aggttattga ggcagtagat ttgttgcgct ttgcccttgg ccttggccag cgcgggcagc
    10381 agcagggcgg ccaggatggc aatgatggcg atgacgacca atagttcgat gagcgtaaaa
    10441 gccaggcggc gacaggtctt gcgcgtgcct gaggcaggcg cggatggcaa aggcaccaac
    10501 actttgtccg ggcctgaact cacgcaggag gtttcggtct cttggcggta ggtcgaagtc
    10561 ataagtttat ggttatctgg atgcagcact agttagttgt tcgcatatat cttgccaaca
    10621 gttctaccgc aaattatcgt aaacgagagg aggcgtcaaa tactaaaaaa ggccgtgtgg
    10681 gcgatggttc aaaatactcg atgagttcac gccgcacgcg atctttaagc caggcggcgc
    10741 tcggagctat ggcttcgcgt ttgagcgtgc tcccaaacgc ctccatggcg gcattgtcgc
    10801 agccgcagcc cccgcggctc atgcggaccg cctggcgcgc cgtctccccg gcccgaaccc
    10861 aggcatagca accgttgcga gttaactgcg gcacccggca aagttcccgc accgggtttg
    10921 gggggattag cgcatccaca ggcttggcgg ccaccccgta ttgccgcttc ccgtcgcgca
    10981 agttcccaag gccaatcccg atcccctggg cgacttgcgc cttgctctta ccgctgctct
    11041 ggagttgatc gagcaccgaa cacttgagcc cctcacggta gcgtttccgc ggatgccgtc
    11101 tttccgagct ctccggtatc atattaccgt tctctcagac ccttgaccca gtatccactt
    11161 tttcagggga aggactccaa ctcgcccacc cacccgcctc cggctttcga tttccccctc
    11221 agtctcgctg tttcaaccgc gaaaaccacc ctaaaaactg ccccccaagc ccatcggact
    11281 cccaatcccc ccaaaaaacc tcaactcctt gcaatcacgc cacttccagg ctgccaacaa
    11341 gccgtgtaca aggggtgtac aaggggtgcc caccggagat acataagatt tatcgttgta
    11401 tacccggtgg gcaccccttg tacacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnncaccc cttgtacacg gcttgtgaat ggctatggat taggctgaaa agacgggagt
    11581 taagtgaaaa acggcggttc ctctacgggt tcggtaaaat agatcagaag gatgagctgg
    11641 cctgaggcat gaagccagaa gccttatttc atgagctgtt gagactggga ttaaactggg
    11701 caggagccga gattcgactt gaagccacaa tcggcgtggc gtctctgggg gtccgggaac
    11761 ggccatcgct tttggcatcg aaccgcggtc tccaggatga cgggcgaatg ttctgccatg
    11821 accataccga aggactcaac tggcgtcatc tcaataggtt ccaacatcga ggtgaaatcc
    11881 gatgccggtt gctccggggc aaggccaatg tgggcgtatt tttcggatgc acctcctctt
    11941 ggaaggatta agcctccatt tcatcagaga gttcgagggc ttcgcgttgt tgcggatgct
    12001 cttcaagcct gtgggcgggg cttatggcca agctggcttc agcaaagttt gctgcgtgag
    12061 aggggacgag ttggggatgc gcaacggtta gcccggtctc agcgtatttg ccgattgggt
    12121 ggttagcggg tgattttgcc actaataaag aatgtttgac aaaccaaggt attgagctta
    12181 agatggaggg cagagcagga ttggtggcac gaccgaatca ggttatggcc agtgttacac
    12241 aagcagacgc gctggagaag ggccccaccc gtttagtggg ggcgtgcgtg ctcacagatg
    12301 cggcgtggag gatcgtgggt caccggcttg gcctgtcggg gcgggagttg gcgattgtgc
    12361 gtggagtgtt cgatgacaag attgaaatga cgatggctgc cgatcttggc attgcgccgc
    12421 gcacagtgca tagccacctg gagcggctct accacaaact cgatatcaac agccgcgctc
    12481 aactggtgct gcgggtgatg caagagagct taaccttgat ggcagcaggg ccgccgggcg
    12541 ggacgccgca gatttgtgct ggtccgggcg ttgggcatct cccgctccga ctgcgcgaac
    12601 aggcggacgt ctccgctccg tcccgcggag gcaaaggctc gaaacggggc tgagcccttc
    12661 tcccagaacg cgcccggcgt tcgttcgaac tccgagcgcg tgtgagtaac cgtccgtgcg
    12721 cggacccgtg ataggcggcg tcttagcggg agcgccggcg ggtgccgaag gccagcagcc
    12781 accctcccag cagcagcagc atggcagtgc cgggttcagg tattattaca atgctgccgg
    12841 tggacaaacc tcccccgctc gcgccgccga ttccaagact gccaaagtac gctggagagc
    12901 cgttccaact gctggtataa tcctggataa cgaactgcca gatttcgccg ggctgccaga
    12961 tgccatccat gataaagctt tccgcgacca gaggggtgtt cttctgcccc cattgtccac
    13021 cgagcgcagc gcgcctgtca atcatgaaag cctggccggg gtcaaagtcg acttgcccaa
    13081 cccattcgtc ccaattgctc agcgaggtgt ccgcgtccgc cacataaaag acttcgccct
    13141 cgtagaaagt ttgccgggtc aggttccgca tggcgacttg cactaccacg ccatctttat
    13201 aatccgtggg gcacggaatc cacccgcccc acgggacttc ccacgactcg attcgttcgt
    13261 cttctgggaa ggtgggctca tttccaagtt catgagccca ccccgagatg ctccaatcat
    13321 cctgaatagg gtcatggagc gtatagattt tcagcggcca ggctccggca ggcctgacca
    13381 gagtaaataa tgctgccgcc acaagggcta ggtagttaat gtgttttttc atagaaacct
    13441 ttcgtgagta tctttgttct gtgtttaatt tattcctgtt ttattcggag tgtatttaac
    13501 ccggtgaaag gagtggtgaa aagtcggcta ttgagccgtc ggctatgcag ccgacctcgc
    13561 cgcttgcggg ggtgattgcg gagccgggaa agcaccggtg ggatgaaggg agccgaccgg
    13621 agcacgggcc ttcctgtctg ttgccaagcg gagggcaagc tggggagaga ggattgggta
    13681 agtctcttta cacggagtcc ggcgcgggtt gcgattcgcg gtgtggctta ataagctcgc
    13741 tcaacacgcc aatgggcgcg caggcgggcg ggagactggc ccgcctcccg caagcgccgc
    13801 agactggttg cgtcgtggcg cttggccagt cgcgcgccgg tggaatcggt cagcagtggg
    13861 cagtggtaga atcggggcgg aagccattcc agggcgcggt agagcagcag ttgccgcgcg
    13921 gtgctgagca gcaagtccgc gccgcgcaca acctcggtaa ttcgcatcgc ggcgtcgtcc
    13981 acgacgcagg ccagttggta ggaggggact tcggcgtggc gccagacgac gaagtcgccg
    14041 aagtctcggc cggcgacgaa ttgctgcgcg ccgaggtttt ggtccataaa gaagatcacc
    14101 tcgccgtcgg gcacgcggaa gcgccagttg attcgatggc cggcggggcg cgaaggggtg
    14161 gcgggcggtg agttgggcgc cggttgcgcg tggctggcgc caagagggcg gcaggtgccc
    14221 gggtagatcg gttcgtgctc atcctcggca tgaggggcgc gggcggcgga gagaatgtct
    14281 ttgcgggagc aggtgcaggg atagataaac ccgcctgcgc gcaagtgatt aaaagcggcg
    14341 cggtagaggt tctggcgcgc gctttgcgcg tagggtccga aagggccgcc gcaatccggg
    14401 ccttcctgcc actgaaaacc aaaccacttg aggtcttcga gcatggcttg aacaaattca
    14461 tcgcgacagc gcgcggcatc gaggtcctcg ctgcgcagca ccagggagcc ggcatgggcg
    14521 cgcgcccgct cctgggcaat ccagaaggtt cgcgcgtggc caagatggag cagcccggtg
    14581 ggcgacggcg ccaaacggcc ccgataggga gctgggggtg gcagcggagc gggcgggttt
    14641 gcggggaggg cgggggtggg gttggccatt ctaaaagctc gcggctgaaa tgccagccgg
    14701 gcccgttaaa tcagctaaag caaagggcgg atccggggtc gcggcttgtt tgcgcgtttc
    14761 gcgagccatc ccatcccggc aatccccggg caaccgcggt ctttcgggtc atgatttgat
    14821 aatggccgcg ccgtagtcgc ggttcatccg ggcaataaag ctaaggctga tttccttggg
    14881 gcaaacggct tcgcaggagc cggtgaccgt gcaaccgccg aagccctcca tatccatctg
    14941 ctttaccaga ttcaagacgc gacggtcttt ttcggctttg ccctggggca gaaggttgag
    15001 gtgtgagact ttggcggcga cgaaaagcat cgccgaagcg tttttgcaga cggcgacaca
    15061 ggcgccgcag ccgatgcagg cggcggcgtc catggcgcgg tcggcgatgt ctttgggaac
    15121 cgggagggcg ttggcgtcgg gcgtgccgcc ggtgctcacg gagacaaagc cgccggcttg
    15181 gatgatgcgg tcgagggcgg tgcggttgac gatgaggtct ttgatgagcg gaaaggcctt
    15241 tgcgcgccag ggctcgaggg tgagcgtgtc gccgtcctgg aaatgccgca agtggagctg
    15301 gcaggcggcg gtgccgtggt ggccgccgtg cgggatgcca ttgatgacga tcgagcacat
    15361 gccgcagatg ccctcccggc aatcggagtc aaaggcgatt ggctcctcgc cgcgggtgat
    15421 gaggtcctcg ttgaccacgt cgagcatctc caggaaggac atgtcggggg agagattgcg
    15481 ggccgggtag gtttggaacc ggcccggggt ggcggcgttt ttctgccgcc agactttgag
    15541 ggtcaggttc atgcgtagct gcgggtactc atggagattt cttcatactg gagcggctcc
    15601 ttatgcagaa caggaggttt ttccagcccc tggtattccc aggccgcgac gtaggcgaag
    15661 ttttggtcgt cgcgccgggc ctcgccgtcg ggagtctgga actcttcgcg gaagtgtccg
    15721 ccgcaggatt cccgacgatg gagggcgtcg tggcagagca actcggcaaa ctccatgaag
    15781 tcggcaacgc gcccggcgta ttcgaggctc tggttgaggt cgccgctttc gccgggcacg
    15841 ttgacgtttt gccagaactc ggcgcggagc tcggggatgc gccggagcgc ctcccggagg
    15901 ccggtttcgt tgcgagccat gccgcacttt tcccaaagca gccggcccag ttcccggtgg
    15961 agggaggtgg gggtgcgttt gcctttgatg gtgagcaggc gttgaataaa ggcgcgggtg
    16021 tccgcttcgg cctgcttgaa ttcggcgcgg ctggtggccg ggtgggggcg tttggcggtg
    16081 gccaaatagt ggccgagggt gtaggggagg acgaaatagc catcggccag accttgcatc
    16141 agggcgctgg cacccaggcg gttggcgccg tggtcggaga aattggcctc cccgatgacg
    16201 aatagccccg gcagggtgct catgagattg tagtccaccc acgtgccgcc catggtataa
    16261 tgcacggcag ggtagatgcg catgggcgct tggctggcgt tttccccggt gatcttctca
    16321 tacatgtcga agaggttacc gtagcgatcg cggatgaccg gctgccccag gcgctgaatg
    16381 gcgtcggcga agtccaaata gacgcccagg ccgccggggc ccaccccgcg gccggcgtcg
    16441 caggcttcct tggccgcgcg cgaggagatg tcgcgagggg caaggttgcc gtagctgggg
    16501 tacttgcgtt caaggaagta atcgcgatcc gtttccggaa tggcggccgg gggtttggcg
    16561 cggtcggctt tgttcagggg cacccagacg cggccatcgt tgcgcaggga ttcagacatc
    16621 agcgtgagct tggactgatg ctcgccgctg acagggatgc aggtgggatg aatttgagta
    16681 tagcaggggt tggcgaagaa cgcgcctttc ttgtaggcgc gccagatggc ggtgacgttg
    16741 cagcctttgg cgttggtgga caggaagaag acattgctat agccgcccgt ggccagcacg
    16801 acggcgtccc ccgcatggga ggagatttgg ccggtgacca ggtcgcgcac gacgatgccc
    16861 tgggcgtggc cgtcaacgac cacgaggtct agcatttccg tgcgggggaa cattttgacc
    16921 gtccccaggc cgatctggcg gcagagggcc tggtaggcgc cgagcagcag ttgctggcca
    16981 gtttggccgc gcgcgtagaa agtgcgcgag acctgcgcgc cgccgaagga gcggttggcg
    17041 agcaagccgc cgtactcgcg cgcgaagggc acgccctggg cgacgcattg atcaatgatg
    17101 gcgccgctga cctgggcgag gcgatagacg ttggcctcgc gggcgcggaa atctccgcct
    17161 ttaagggtat cgtaaaagag gcgatagacg ctatcgccgt cgttggcata gttcttggcg
    17221 gcgttaattc cgccctgggc ggagatgctg tgagcgcggc gcgggctgtc ctgaaaacag
    17281 aagcatttga cccggtagcc caactccgcc agcgcagcgg cggcggaagc gccggccagg
    17341 ccgctgccga cgacgaggag gtcaaacttg cgcttgttgg ccgggttgat cagtttgagg
    17401 tcgaatttgt attgctccca tttttgggcc agcgggccgc tggggatttg ggcgttcaag
    17461 ggcataggac ctcctttcca tagccgcaga gaatggcggc cggaatggcg cagtagccaa
    17521 gaaagagcag cagggcgagc acgcgggcgg cgcggtcgag gcacgggcgg taggcgtccg
    17581 ttctccagcc gagcgagtga aacaagctgc tggcgccatg actaaggtgc aaccccagaa
    17641 gcgccagtcc caggacataa aaggccgaga ctatgggttt gcggaagcct accaccatca
    17701 tcttgaagat gtcgggccgc tccaccgatg ccgggcctgg gagtttttcc gtgaaagcag
    17761 caaagtcctg atgtgtgagg ttgatgaatt ggactttggc cgtgtagtgc aggagatgat
    17821 agccgaggaa cgcaaatacc ataagcccgc tcagaagcat ggtttgcgag gcaaaagtcg
    17881 agccgaaagg ctggtattgg gcgtatgcca cagggcgggc ggctttgttt tccagcgaca
    17941 gggtgattcc ggcccagata tggagggcta agatcaccag catgcccatc cgtaccggcc
    18001 agatcaattc ggggttggac tggaggaaat gagcgtagcg attaatgatt tcccgaccga
    18061 ggaagatttg taaattgccc gcgaggtgtc caaggacaaa gaggagcagg cagccgcccg
    18121 tgatggccat gatgtatttc ttaccgactg acgactgaaa aaggttcgta ataaggttca
    18181 tcggccacca ccctcggcct accggaacgg cccggcgttg ggcttggaaa gaagtctaat
    18241 cagaaaaata ccgccgtcaa gagaaccggc cgcatttgcg cggttgaggt gcgtgctttc
    18301 ggaaaagcga aatacatcca tatggcaacc ggagggggag ttggcaatat gccgggcttc
    18361 ggatcaggac gccgtcggga ggcagggcag gggaggcgcg gttggctggt cccactcgtt
    18421 taacccacgc tctggggttg ccgttgccgg ggagaaatgc agcagttcca aggcttctcc
    18481 gatgagataa agagagccgg cgataattac gaaatcggtg ggcgtggttt gcgccagtgc
    18541 tgagcgcagc gagggatgcg ggctggtttg ggcatgggga ttaatgcgcc ggcagagtgc
    18601 agcgagttct tccggtgcgg cgctgcgctc gctgggaacc ggcactggca ggatgcgcgc
    18661 ggccagcggc gcgagaattt cgcacatgcc gggccagtct ttgtcgcgca agatgccgag
    18721 caccagggta atgcgagcgg acggaaagta ttcctttact gcggtggtca ggatttctgc
    18781 gccgccaaca ttgtgcgcgc cgtcaagcaa gaccttttgc ccatccgggc gcgtcaggag
    18841 ttggagccgt ccggcccatt gaacttggga taatccggcg cggatgagtt catcgctcac
    18901 aggaaagtgt gcggcgagcg cgcgcacggt ggccacggcc acggcggcat tcaggcgctg
    18961 atgccggccg gatagtggca gcgagaggat gtccagtggc ggatggagcg catgagcagg
    19021 cgtcagggtc gtaagcgggg cttgcaaccg gcgggcggtt tgctcaataa cgcggagagg
    19081 ctcagtgcct tccgccgctg tgataacggg aattccgggt ttgatgatac ccgccttttc
    19141 ggcggcaatg ctggccagag tttcgcccag ccatttctgg tgatcgaatt ggatgttggt
    19201 aatgacgctg gcgagcgggg tgacgatgtt ggtggagtca aaccgcccgc ccaggccggt
    19261 ttcccaaatc accaggtcgc actggcgctc ggcgaagtag cataaggcca tcagggtgat
    19321 cacttcgaag aaggtcgggt ggtcggtttt gggggcaggg ttggcggtgc gtggggcggt
    19381 cgaggcagca ggccagcctt ggcgcagtaa cgactgcaac tcggtggcca ggcgtgcaat
    19441 gtcagcttcg gagataaggt ggcggttgac ttgaatccgt tcgccaaacg cgaccaggtg
    19501 cggcgaggta aacaggccga cgcgtaaccc cgctgcgcga tagatgctct ccagcatggc
    19561 gcaggtggaa cctttgccgt tggtgccggc gacgtggatg aagcgcaggc ggtgctgcgg
    19621 attgcccgcc agtgcggcta gcttgaaggt gttttccagc cccaacttgg cgccaaacca
    19681 gcggaggtta tacaggaaat ggatggcgtc ggaataggtc atggattgaa aaagcggaat
    19741 cagcctgccg tctggccggc caaaaggtgc tccacgcgcg ctacatccgc cggcacatcc
    19801 acgcctgcgc tatcatgagt cacttgcacc accgcaatgg gaatgccgtt ttcgagggcg
    19861 cgcaactgtt ccagcttttc ggctctttca agtggggaga tggggaggct taccagtcgc
    19921 agcagcgtgt cgcgccgata cccataaatg cccagatgct tcagaaaagg aaacgccgcc
    19981 aactgttcgc gtggcgaagc cgccgcggtc tcacgcacat aaggaatcgt gcgtcgggaa
    20041 aagtaaaggg cgcgtcctgc ggcattgacc acgaccttta ccgtattcgg gtcgtcatag
    20101 tcagcgaggt tggtgatggg ggtggcggcg gtggacattt cggcttgcgt aagcgcggtg
    20161 gcgacggcgt tgatgaccga cggggcgatg agcggttcat cgccttgaat attgacgatg
    20221 gcgtcaccgg cgcgccgcgc cgccacctcg gcgatgcggt cggagccgct cgggtggtcg
    20281 ggtgcggtca tttcaacctg gcaaaagcgt tccacggcct ttaggattcg ctcgtcgtcg
    20341 gtagcgacga tgatttcggc gagtgcggag gcttgctggc aacgttcgac gacgtgctgc
    20401 aggagcggcc ggccggcgat cagggccagc ggctttccgg ggaaacgcgt ggagccccag
    20461 cgcgcgggaa taatgccaat aattttcatt gtgctctttg tagccacagc agtcgaagat
    20521 tgcacggacg aaaagaatta ccgcgccggt ttcgactggc tttttgcaca cggagcgcgt
    20581 atgctaaacc cgagctgaag gacaggttaa acatgcattt ggaactgatt ggaccagacc
    20641 cattcgcgcg ggacgagcag ggccgacaga ttactcgaat cggcagcctg tttccgtctt
    20701 atggcgcgct ttggacgcaa ccgccggggg tgcatgcgct gcagcgactg ggcttcattg
    20761 ggcacgtcaa ccaggggcgc gtcgccaggg ggttggcgcc cttgaatcga gaagaagaag
    20821 aggaactatc agccgagtcg gtggatcttg tatttgatcc cgaacatatt ctgattcggc
    20881 ccaaccccga ccgcatggac ctggcgtttg ccggggatga attgctccag acccgggtcc
    20941 ccaagcggca ggttaagttc ctgtcggttg cggatcgccg ggtaagggag gcaattaaac
    21001 agcgtggaga atgttggcgc ctcagttcga ttcccaagac cattgaggcg aagacgcggc
    21061 tggtgctggg gtcgaaagta ggcattcatg gccaacccat ctatttctac aatcgcttga
    21121 ccggcacccg gtggctgacc tgccaggagt ttgcggaact cggcaaactg gatgatgcca
    21181 acttggcgtg ccacctccgg gaaatagcgg tgcattccac cttgcgtaat cggttaggcc
    21241 atccggaatt ggatttcttt gccgtcgacc agcagcagtt cggtgcgcct caatttgctg
    21301 gcaccgatta cacccaactg ggcagggaac aattgcgcgc gctttttggc gatctttgcg
    21361 cccgttttag tgccgctgtg ggcaaggatt tttgcaccga cgatcctaaa aaccgtgcct
    21421 ggagcgagcg gattttggcg acgcttttcc tggagggcaa tgagacggag acggaggaga
    21481 ttctcagcgg gctcagcccg gaattctacc tccaggtgga gtggttgccc ggcgggcgct
    21541 tcgaggaggg tgagtttctg cttgacccga tatttgatga tgcggtccgt catccggagg
    21601 acgaggagtt gcagcggctt tgtgattcgc gcgcgcaagg catcatcttt aacttcatcc
    21661 gtgaatatgg cgacctggat tacatcaatg tgggctgtgt ggcggaatcg ttatcactgg
    21721 ggcggcctca aaagaacagt cgccggggcg tgtacctggt cgaactctat tcccggagtg
    21781 agcgcgccgt tattaaacgc ttcctgcgtc tccaaaaatg gagtgtttgg gagcgcctgg
    21841 atgaagggaa agatttgctg caggcaattc gggaaagcga cgactatacc gactattgcc
    21901 tggaccgccg tctgggctgc caccagttgc ggatgaacct gatccggcgc gttggcttgc
    21961 ggcgcctaag tgaaatctac cggggttcca acgctcgatt ttatggcgaa cccatccgta
    22021 cgttttattt cgagcgggag tacttgtcgg gcattgccac cgataaactg acgccggaaa
    22081 ggtatggtcg cccgggatac ggagctaggc tggctgaact tctggggcgc gcagcggctt
    22141 ccaacttgat tgtggggcgc gctcaggaag cagaggaggg gaaagagttg tgcccgcttt
    22201 tcgatgatgg cgatgaactg gtgcgggagg gcgatgatgg attgccttcg gaaatcctgg
    22261 ttgtggacta caccggcgcc tttgttgaat ataagctgcc cttggagaca ttcgccgccc
    22321 gctacgcgca tcctgttaac agtcgcggtg aggctattcc tgatttgcga gcgttcgcca
    22381 ccgcctacct tgacgccctc cgtgaccagt tcctgcagat tcaaggtgac taccgcattc
    22441 gccgccgcgc gtttgacaca cttttccagc actgcaagta cgacccggag ggcagctttg
    22501 cttaccgttg ggaatgtgtg ctaagccggt tggaccaaac taatccctat gcgcttgtgg
    22561 acgccattcg tcagcatatc gagtgttgag ggcgacgcca cgcaccggac caacagagag
    22621 caatggcaac tcacttgcgt ataaccatgc acgaaaacgc ctgatggcac aatttgcgcc
    22681 atcaggcggg agaggtcggg aatgatttcg actgccttgt cgcaggtcgc gacgggaggt
    22741 tagaatttgt acgccgtgcc ggcgattagg cgcagatcat tatgatcgtt accagcagcc
    22801 ggttcgctat cataaatgtc ctggaagacc acccgcaagc tccatttctt ggtgatggcg
    22861 gtgtcaatgc cggcctcgaa tgtgaggagg tatttttccg tccaccggtc caattgcggg
    22921 acataagtgg cgctttgcca gatcttggtg gtatcggtga gtttgtgctc aaagcgttcg
    22981 gccaatcgga gaccggcata ggtgtctgac gattgatttt gatacttttc aaacaccact
    23041 gacgggccag cctcaaatgc cagcgaggtt ctggcttcct tgatcaggta atacccggcc
    23101 aaggggctga cggtgacgcg gtagctcaga ttcgctatgc cgtcgtagtt gaaatcagcc
    23161 cgcaacccaa cgtagaagcg ttcgcttaac agccggttgt attggccgaa ggcgttgaca
    23221 aactcagtgt ttcgcacctt atgatcctca ccgtaaccac cggcgagacc aagtgcgact
    23281 tcgtcgtgct cccacttccg cttggtatcc aggctcagcg tggccagcag ggtttcgctg
    23341 ttcccacgag tcaacgtcag gccggcggcc gccgtggtct cccaccggtt ggtggatgga
    23401 ggggcgcttg aggcggacgc ctgctgggcc tgagtcgaaa tcaagctggt tccgagcaaa
    23461 agagcagtgg cgagagccaa cccacgacgt ggactgcacc gctgcgaagg attcatctgc
    23521 gtatattcag ggatcattat tattcgtatg ttatgtttca ttattggttt attgcgtgaa
    23581 aagacagttg cactattcga ccgtgacgct tttggccagg ttgcggggct tgtccacgtc
    23641 gcatccgcgt agaacagcga tgtgatacgc aagcagttgc agcggcacca cggtcaatag
    23701 aggctgcaag atatccagcg tttgcgggat gtagatcacg tcgctggcct tgtgcttgat
    23761 ttcggcatcg ccttcagtcg cgaccgcgat gatggggcct ttgcgcgccc gtatctcttc
    23821 aacgttgctc atgttcttgt cgtacatcgc gtcgcgaggc acgacgaaaa cgcttgggaa
    23881 gtcgggtgca ataagcgcga tggggccgtg tttcatctcc gctgaaggat aaccctcggc
    23941 gtggatgtag gaaatttcct tgagtttgag ggcgccttcc agggcgaccg gaaagttgaa
    24001 ctgacggccc atgtaaagga agccggttgc ctggtggtac tttttagaga tttttcgcaa
    24061 atgcgcatct cctgccagaa ttttttcaat cttgcccggg atggtctgga gttcgcgcac
    24121 gagtttgcgg cccgccgccg gggaaagttc gccgcgctgg cgcgccaagg caatgcccaa
    24181 tagcgtcaat tgcagcacct gcatctcgaa ggctttggtc gaggctaccc caatttcggg
    24241 gccggcacgc atgtaaatat ccgcatcaga ttcacgggcg atggtcgaac ctacgacatt
    24301 gacaataccc agcgtacgtg caccctgggc cttggccgcg cggatggccg ccagcgtgtc
    24361 ggcagtttct ccggattgag agatggagac gatgagtgtg ttcttctcga tggggcgccc
    24421 gcgatagcgc atttcgctgc cgtattcaac ttctaccggc aggcgggcga gggtttcgat
    24481 gacatactca cctaccagcg cggcgtgcca gctagtcccg caacccacaa gaatgagccg
    24541 gttgatcttg gccagttcct tgggcttgag ggccatttcc tcgaactcga cattgccctt
    24601 tgcggggcga agggtgcgcc gaagcacctc ggccacgcgg ccgggttgct cgaagatttc
    24661 cttgagcatg tagtgagggt gtttgccgcg ttcagcttcc tcagccgacc aatccacgga
    24721 actgattggg cgctggatcg ccttgccgcg ggcttgcagg gcaaagtctt tgtttgtcag
    24781 cgtgacgatg tcgccatcgt gtaaatagac gaccttgcgg gtatgcgcga caatgggtga
    24841 aacgtcgctc gccaggaagt tttcgtcctg gccgaggccg acaaccagag gagaaccgcg
    24901 gcgcgcaccg aacaggtgac cgggcaagcc ctggtgcatg agggcaatgg cgtaagtgcc
    24961 ctcgacttca cgcgtagcgg cgaagatggc ttgtgtgacg cgctccggcg tgacttcgcc
    25021 tttccgggcg agtgcctggt cgaggtgatg gccgatcaaa tgtgcgagca cctcagtatc
    25081 ggtctgcgac aggaagcggt ggccaaggcg ctgaagacga tgccggagcg tggtgtagtt
    25141 ttctatgatg ccgttatgca ccagcgcgac cttgccggac tggtcaaggt gcggatggga
    25201 attgatgtcg ctgggcaggc catgcgtggc ccagcgcgtg tggctgattc cgagcgagcc
    25261 cttaaccggt ttcgttttga gcgattgcgc cagtccttcg acgcgcccca cttgtttgcg
    25321 cacatgaagc gttccgttcg tcaggacaca tatcccggcc gagtcgtacc ctcggtattc
    25381 gagccgccgg aggccttcaa tgagcaacgg cgtcgcttct ttcctgccta catatccaac
    25441 tatgccacac atgttcttta ctctttttgt ttaggagtct gtttcggaaa caccccgagc
    25501 tgcgaggtgc cagtttaggt ttgcttgagg atccactgcg ccgccgcggg caaatcgtcc
    25561 acgacaacgg cggcggccag tttgtcggca gcttcctgct ccagttccgc cccgtagcct
    25621 gtgcggacca gcagacactg cttcacgccg gcattccagc cgcattccaa atcaatcagt
    25681 ttgtcgccaa tcatataact ttgcgccagg tccaccccaa actccgcgcg cgcatcccac
    25741 aggaattgcg gcgagggttt gcggccccgg ctgggcgcac caggcgcctc gggcgcgatg
    25801 taaatcttct cgaaccgcac ccggtcacgg tctaactcac ggaggagata ctggtgtatt
    25861 tcatggactt gcgccagtgt aaaatagccg cgccccacgc cggactggtt cgtcacgagg
    25921 aaaagccgga atccggcttg gcataaaagt cccagtgccg ctccagctcc gggaaagatt
    25981 tccacttctt ccacccggct gagatagttt ttttcggcga tgatggttcc atcgcggtcg
    26041 aggaaaacag ctcgattcat ggtgcggttg gggggagtaa taaagcaaaa ccgcggttag
    26101 ggaaataata aagccgtcgc aaaatcgaac tgtgaacagt ataccggtga tgccaggctg
    26161 tccgccgcgt attttcttgg acgtttgtgc cgccgccacc tccggctatt cgaatatcag
    26221 gcccttttct gccgtgaacc gagcaattgc cgctcgcccc tcgtagccga cattggggcg
    26281 cttattattc tgtccgatca gaaagcgttg attgcccaac cccacgatac ccagcgacgc
    26341 atcgaattcc cagcggtccg tcagttgcag gcggggattc gcgcgcagca gcaccttggg
    26401 ggtgccgtag cacccaaacc accacgcccc ctccgcgaaa gcaaccgtct ggatgcccat
    26461 caaggtgtag ccgctggcga gcacgtgccg ttttctaaac gcaaacttct catcgtattc
    26521 gtagaggtag ttttcattta cgccttccgg cagtccgccc accactatga atcttccctt
    26581 gtgataagca atgccgccgg ctccatgctc cacttcaggc acggggtgcc gggctcgttc
    26641 gatgagcgta tcggcgtcgt acacaaagac ccacgaattc gcgtagccgg ccgggcggtt
    26701 aaatgcgccc aggttcaccg cgacaaaaac ttcgcccaga tgatggcata ggtcgccatg
    26761 atggctggcc acctccaggc gcttgaggat acgcccgtgc cggtcggttt tgaccagtgc
    26821 gtccgtccaa ctccagtaaa gcgcatcgcg ttcattggtg cagatgccct gcagatgtcg
    26881 ttcgtaagcg ccctcgcagg tggctgcttg aatcgccgcc gtccccgcgg gctttttcca
    26941 gaaggtgttg tccagcacat actgacgcaa ttcctccggt tggtttgggt tggcgatgag
    27001 aacccggagc cggggggcgc cctgggccgg cgtttcaacg atccacgagg aaagggtgca
    27061 gccattggtt cccgtacgcc acaagctatt attcggacca ccatgccggt ctcgactcat
    27121 gctggcgaat tgtgccgtgt cgaatggccg gggagccgcc tgcagcaggt ccgcaatccg
    27181 cttaacgcgc acacaactgc tgcggttggt cttgagatta aatttctgca ttgtctcgtc
    27241 gaggtaatgg tttgcatgca ccacggtgcc agttttaacg gcgcgcatcg agtagcgccc
    27301 ctgcagaccg acttcaagca tgaggatctg cttgcggtca gctatcatca cgaaggtggg
    27361 ccgccgctgc gaaaagagct tatcctgttg ggcgagcacc tcgtcgcaat tagcgcagtt
    27421 cgccagcagc gtgctgataa ggctgccttt tcccggttga gcctcgcgcg tctgtttggg
    27481 aatggttccg gccgaggcgg tcaccacggt cagacccttt tcgttgattc cctccttaat
    27541 cccgggcgca tcgtttcctt cagcatacag accgaaataa gcatagccat ttgtaggccg
    27601 ctgcattttt agcacctgca catggtccgg cttccaatca cgattcttgg aaatgatagt
    27661 accccctccc gcgtcgactc cagcagcccc ccaaagtgtg caggcgtgta ccgaagcgca
    27721 ggatattcct cccaggaaca acagaattaa aagctgccaa agagtgaaag gccagtttcg
    27781 gtttataaac cgagcgctcg gtcccggcat cgctaaaaca gcttgcgcca tgattcaatg
    27841 ggggttgatt tcagcggaag tttcagcccg tgtatttcac atatttgatc tcgcggatgt
    27901 ccagggttcc ggccctgggt tcataaaacg gcctgccttt gcgcacgccg acgaggccgt
    27961 agccggaggc ggtagccaga aagcagcgga aatcgccccg gagacggggt tccaagtaca
    28021 gcaccttttc tacggcgtca tagcgcgcgc cgctcaggcc aaagagcagc gcataagaag
    28081 acatcgcgcg cgcataccaa tggccgcact cgtattcatt gaagggattg cgcacgcggc
    28141 catcatagcg gtcgcggcaa accctcacta cttcaagacc ttcggagacc agccccattc
    28201 gcatcaggtg cgacgccacc tgatattcga tgccagtcca aacctcgttg gaatagacaa
    28261 atggcagcga gagttccccg ccttttggcc aggtgcagag caccaggccg ccttccgccc
    28321 cgcaagcgta ggcaggccgc tggggattgg cgaaatccga caagtcgtgc ttgaggttat
    28381 acttatggac ggcttgcaag tgactggcca ccttgggtgc cgccagcacc tgccctacgc
    28441 cgcataccag cgccaaccac gagcccaaca cgccgtcgga aaggcagccg gtgccatatt
    28501 gatagttcgg cccttctttc tcaaagaccg cccgggcttc cggcgaaaag ttccccgtca
    28561 ggttcttgtt ctccagcggg ttgccggctt ttaagttcgt ccagtcaatg cgctgaatga
    28621 aatactcacc gtcgaataac tcggactccg tccggcgaat accggccgcc agcaattggg
    28681 catagagtgg cacctcgtcg cccaacgctt tgcccatcaa caccgcggct tgcagtgccc
    28741 ccagatagaa actggtgcac atgccgtccg gcccccaaaa ctcaatgtcg taggtgttgt
    28801 ggtgtggctc ttcgatcact cctttatgcc ccggatccca ggtcttaatg caaaactcca
    28861 ggctgcgttt gactttaggc cataatcccc gcagccattc ggtatcacca ctgattcgcc
    28921 attcgcggaa taccttcatg attccaccaa gctgcccgtc ggcggcggcg tggaaatcat
    28981 gctcgacggg ccggatcggg agtgcgctgc ggaactgttg atgcccggcc tcgtcctgcg
    29041 acgggccaaa ttcggtctcg cgcagcgtcc gctccaggcg cgggaacagg tgcgggatgg
    29101 cctgcgcata attccaaaca tgcgtgcagg agccgtgaca acaacccgcg ttgtcatggc
    29161 atccctccca cccccagagc cggccatcgg tctggcgcat cacggttggc gatttaagga
    29221 tcgtcaggtt ggcggcgact gcttcgagga cctcgagcgg cagagtggag gcatagaagc
    29281 aatcagcaaa ccggctcgcc ttttgccgca gttcgtcata gtgatcccgc caatagaacg
    29341 agacgccgtt gatgtctgta aagcgtcccg cataccacgg gcggtaatgg tcccgcgaga
    29401 ccgcttcggg cttcaatccc gccgggtccc ggccgatgcg caggttggtt tggcctacaa
    29461 accaggctaa tcgcaccgtg atcatttttg ctgcgccggc agcaagctga aaagggacaa
    29521 acagtgtccc gcccggactg ggtctccccg tggtcaccgg cggccgctcg aaacatgcgc
    29581 cttcggtgat atctttccaa gccagcgtca aggcatccca ccagccgccc cgaaaccagg
    29641 catggtttac cttagtgtcc gcttcggaca ccgttgccga aaacgcggct tcgtcccacg
    29701 gatgatccgt cgagccgcct ccccacagga tgaacccgcc cgcgatgggg cgcaccgctt
    29761 gcggattgcc gcccgtcacc atgaagttcc tcgcattgaa agaaaatacg gcttcgagcg
    29821 gtttaccgct gcggttggtg aactggtatt ccaacccagc caccggcagg ctggaattat
    29881 ccgcatcgcc gggttcgaag gggctccaac ccgtaattgc aacgctcaac ggaagttgcg
    29941 gatcggtaag cgtcacggtg ccaaacggga aacgggcttt gaaggaggcc tgggcaaagc
    30001 gcggcaggcc gtagctggtg ccgcccaggc cattgccggc cccctggggg ccataaagtt
    30061 tgcgcccggg aatcggcccc tccaacacgc gcgctgcccg cggttggccc ttgactgaaa
    30121 tggcagcaaa aacacatggc gaattaaaca tgtccggccg gttgcgcagg gagaaatgcg
    30181 agagcatccc cgtgccttca aggcaaatca tgcctgcccc aatgcctccc aagggaaatg
    30241 ccacctggtt taagtattcg ccagaatatc ctccgttgta cacgcgcccc gtccgccggc
    30301 cagttggacg cgcgtcccgt ctgccggagc gggaaggcac cgcctgagcc ggggggaccg
    30361 tgcccagtcc gccgaccgcg cccaccgtcg cggccgacaa ctggagaaac ttgcggcgag
    30421 gcatggaggc atccgggtgt gaattggcag ccataatcgt tctttcaagt attcagtgcg
    30481 cgctacggct tctttatgcc tcaaaagcat gggcagttca agccgcgttt gcgcgaggcg
    30541 ttcgccggaa gagcgcgttc cggccattgt ggggatgatg tcagggcatc gcaagggcag
    30601 gggaccaacc cgggagtagc tcgacccgac gcgccgccga agtggcgggc tacaccttct
    30661 cggccttctc gtaaacgatc aactcgccgg agccgcgcag gtcggcagga tggtaacgcg
    30721 ggatgggcat ttccagttgt ttgaacgtca cgggcgccac ctttttgtcc accaacccca
    30781 cagctacagc gacgccataa atgatggctt caggccgtgg cggacagccg gggatataga
    30841 aattaaccgg cacgagcttt tcaactggcc ccagcacgga ataggagtca aaccagatgc
    30901 cgccgccgca ggcgcaggag ccgatggcaa acacgagttt tggggcgggc atggcttcgt
    30961 aagcgcgctg cagcgctttg gcggtggaac gcatcgccgg gccctggcag agcatgacgt
    31021 cggcatggcg gggtgaaccg accagtttga tgccgaagcg ctcgacgtca taatagggag
    31081 tcaggacatt caaaacttcg atgtcgcagc cattgcaccc gccgctgttg gcatgataca
    31141 cccacaacga gcgcccaaac atcttctggc acaggttctt tagcatggcg attgtccgca
    31201 aaacagggcc agaggcgcta aaatatgggt gtttgaactc cgtagtccgg attgaaacac
    31261 cgcaaaacct tctccttccg ttagagccgg atcgttcatg tagattagac cttcaaccgt
    31321 cttcatcctt ttagcgccat tgggcggatc tcgcaggcag ttttctcatt agtccaggtc
    31381 aatgctgata tcgtccacgg ccgcttcact cgtcaggtaa gcttgcgatc ggaaaatcca
    31441 gcgttcgttc tgcacgtcgt cgaagcggta gcccttgagt ttcaactgtt ccaagggtga
    31501 gggttctgtg aagcagcggc cgcagcgctg gcaggtgctc atgaacagct ccagccgttg
    31561 gcgaatatcg ccgatgcggt cggtggcggt ctcgaactcg cggctcatgg tgatggcctt
    31621 ttcagggcat acatccgcgc agcgcccgca gtaggtgcag cggcgcccga ggtattgcag
    31681 gatgcggatt tcctggcaga catccacgac caggatttca cgcgcggggc aattgctggc
    31741 gcaaccggcg cagccgatgc acctggccgc gtcaaagatt ggccgtccgc ggaatgtttc
    31801 cggcacgggg cccgcctgcg cggggtacgg cagcgtgacg cgccccgctt tgaggcagac
    31861 cagggtttcc tgcaatttgc ttaagagaga catgcgagct tttcagtaac cataacaggc
    31921 gaggatcaaa gctcccagcc ccacgcccag cagagcggcg aagaagcgaa gagcctggtc
    31981 aatccggtag cgggcgtgcg tggccgccac gagcgtcacc agcagcacca ggccgcacac
    32041 tttgagccaa aagagcagcc agccaagcgg gaacgccagg gccgaacccc atggcacgaa
    32101 caggttcacg aacaacgcac tgtaaatgac gagccgggcc atttgcgcat acttgaacag
    32161 cgccagcttc gggccggagt attccatcag cggcccttcc atcaactcgg tttccgcctc
    32221 ggaaaggtcg aacggcaccc gctgcacgaa ggcttggaag gataacagga ttactcccag
    32281 tagaatcagt cccgaccagg ggaaggtgtt cccggcatac acggagccat tgaaaaccgt
    32341 atccagccgg aaagagcccg tgtggatggc cccagcgata atggccacgg cgaacagggg
    32401 ttcgagtgcg atcatggtca tcatttcacg gctgatgccg accagggaat aggtggaccc
    32461 ggccgccaaa cccgccagca acgtgcagat gccggagagc gtcaggagat agatcaccag
    32521 aatcacatcg cccgccccgt tcaaaggcgc ggcgaaaccc attggcacca gtccggcaag
    32581 cgtgagcatg gtggccagcg aaaggcaggc cgcgaatcgc tgcatagcgg gcatctggcc
    32641 ggactcgagg tcctccttgc ccagcaattt gaggagatcg aagtaaggct gcgggagcgg
    32701 cgggccctgg cgcgattgga tttgggccgt gactttgcgc aacacgccct gaaacagggg
    32761 ggcgagcagc agcacgagga cgacgttgac caggaaaccc aggatgacgg tgacggggct
    32821 catatcaggt ctttctcacc agccggatga gttcagcctg cgaccacacc tgcgtcgcgc
    32881 ccgatttcag gtcaatcgtc tccagtcgat ccgtgcagga aaagcagggg tctatgctgc
    32941 cgagggaaat ggtcatgtcc gccagttgct ggtccttgat catcatcggc actgcctgga
    33001 gattctggta ggtgggcgcg cgcacgcgcc agcggtgtgg acggttattc tcgccggtaa
    33061 tgacgaaatg atggctctcg ccgcgcggcg cctcgaccga agacagcccc aggcggccgg
    33121 gcgggagttc atccgggatg gacgcctgca gcggcccctc cggcattttt tccaggcact
    33181 ggcgaataat ccggatggat tcgaacacct cgttcatgcg caccacgacg cgcgaccaaa
    33241 catcgccgcc ggtatcagtg atgaccgcga aatccacgcg gtcgtaggcg gcatagggat
    33301 gatcgcggcg gcaatcaata tccactcccg ccgcgcgcgc gaccggcccg attagcccga
    33361 gggatttgat cagcgcctga tccgccacgc ccacgccgcg cgtgcgtttt tggatattct
    33421 tgtcccgact cacggcggcc acaacctcgc gccattccct ctccagcttg tccagcaggt
    33481 gccgcagttc cactttcacc tcgggcgtaa tatcccagcg tacgccgccg atgacgcaga
    33541 gcgcataggt cttgcggttg ccgctgatct tttccgcgat ccacatgatc ggctcgcgga
    33601 tgcgccagct ttgcatgaaa agcgtgtcga agcccacgat gtggcaggcc agcccgaccc
    33661 agagcaaatg gctgtgcagc cgctcgattt cgagcatgat cgtgcggatg tattcggcgc
    33721 ggggcggcgg gcgcagcgcc acggccgttt ccaccgcctg ggcataggtg acgttatgca
    33781 cgcagccgca gatgccgcag atgcgttcgg ccattaacgg gatgtcgttg taggtgagga
    33841 cggattcagc cagcttctcg atgccgcggt gcaccataaa gccgcggtac tcgcagccgc
    33901 gcaccatttc gccctcgacg tagagccgga aatgctccgg ctcgtccagg gtcggatgga
    33961 agggcccgaa cggcaccacg gtgcagcccg ccggcgggtc gtcgaaggtg aactcggcat
    34021 tctcatcgaa gtccgccggc acctggttcc agggcacgtc cttgcggagc gggtgcacgc
    34081 cgtcgggcca gccatccggc agcaccagcc gtttgggata gggatggccg cggagctcga
    34141 tgccaaccag ttcgcgaaat tcgcgctcgg cccagttggc gccgggcacg gcgggggcga
    34201 tgctgtctac ctccgggtgg tccgcgggca ggtgggccag catgctgcac agcagacgat
    34261 cgcggtcgaa agcgaagtta tgggcgacga gaaacttgcc cgagaaaggc cggtcatcga
    34321 gcccgatgct gatgacgtag cgggcatcca gctcgcggaa aagatactgg cagacggctt
    34381 tgagcgtggc gggctcgaca taggcaaaca agcgggagtc gccgggcagg tccgcgcgct
    34441 ggatggccgg gccaaacttc tccttcaact ggttgagacg ttcgcgcgcg gtcatggttt
    34501 ttcctccggg tgcaaattgc ggttatcggg agccccgccc agccagcgga agtaacgttt
    34561 gatttcggcg tagaagttgt gagcggaata gcggtggcaa tcggcctcgc gcgcgtagcc
    34621 gcagagccac ggcgcagtgg cccggcgcga cgcatggccg atccgggaaa gcgcgtgcgc
    34681 cgccagcagc agcaggccca gcgccgcggc caggaccagc ggggcgaaca aggctgcgga
    34741 ttgcagttga gccacgccgc tccacaggct ggtttgcagg ggcgcggcgg cggccagcgc
    34801 cgtgccgcag cccgaactgc tggtttccag aactcgctgc accaaacgga agcccagggc
    34861 cggcgccacg cccagcaaca cacaggccag ggccagggcg acttgcggca actgcatcgt
    34921 ccagcccact tccagccgcc cgggccgggc cgcccgcgcg ggcaccagcg cgctcgcgcg
    34981 cgaaaggaaa ctcaccccga agaatttgat gaacgaggcc agcgtcaggg cgctggtcag
    35041 gatggcgagg agggcgcaga cggcgaggta tttggcggag ccgcatccct gcaccgccgc
    35101 gacgtagatg gtccatttgc tgataaagcc attaaagagc ggcaccccgg agatcgagca
    35161 ggaggccacg agcgcggtga tggcggtgag aggcatgaac ttcatcagcc cgcccatccg
    35221 gttcagatcc tgggtgccgg tggcctgcag catcgaaccg gcgttgagga agagcagcgc
    35281 cttgaaaaga ccgtggttga gcacgtgaag cagcgcgccg cagaagccga gggcggccag
    35341 cgccgccgcc cggggatctg cggcgggcag gaggctcctc atgcagacgc cggtgcccag
    35401 gaggatgtag ccgacctggc cgatgctatg gaaggcgagc aatcgcttgg actgctcctg
    35461 cttgagcgcc tgcatggtgc ccgtaaagag ggtgatcgtg cccagcagcg cgactaccag
    35521 cccccacggg gccagcgggt aatccgccag gcccgccggc ggcaccagcc agaggaagta
    35581 gcgcatcagc ccgtaaacgc cggtcttaat catcacgccg gagagcatcg cgctgaccgg
    35641 cgacggcgcg gcggggtgcg catccggcag ccagaactgg ccaaacggcc acatgcccat
    35701 cttgatgcca aagcccgcga ggaacaacgc gaaggccagg gcggtcatgc cgggccgggc
    35761 gctgagcagc gccggcaggt tggcgctgac ggtatcgaaa tcgtacttca ggcccgggct
    35821 gccgctcgtg gtggcgcccg ccaccgccag caattcggcg ccgatcatcg tggcggcgca
    35881 ggcgatttgc atcatgacca ggtaccggtt ggccgcgcgg atgttggccg gtttccggtg
    35941 ctcgaaccgg atgagcgcgt agcccggcag ggtcatcatc tgccagaaga tgaagaagaa
    36001 ccacatcatg tcggtcgtgc tcaccaggcc gtacatcgcc gccaggaaga tcaggaaata
    36061 ggggtagtag cgggctgcgc tataaccttc atagttccgc atatacacca ccgaataaag
    36121 cgcggcgggc acggagacca gcgcggtcaa caacaggaaa agcgccgtca ggccgtccac
    36181 atagacccgc agcaccaggc caatctgtgg cagaaggcta aactccgccg agtgcccggg
    36241 cccggagatg aggacccggg tcacggtcga gaccattatc accgccgccg ccagggtgaa
    36301 cacgaacgcc agcccaccag cgaccgtctt gttccgcgag gccagcaggg tcagtcccgc
    36361 cccgccgagg cagaggacaa tggccgccag gactgattgt tcgggcgtca tgccagcacc
    36421 tcccggaggg tggtttccgc aggtttggag ccgggctccg gcgattccga ccgcgccagc
    36481 cggcaattct ccgcgccggc ggtcagcaac gcatgcaaca gttccagggt agtctgtatc
    36541 gtgggcgtca gagcgccgcg gagcttaagt gattccggtt gcacgcccag gagccagacg
    36601 cgtatgcggc cctgcgcctc gatttgccgc gccagcaggc ccagcgaaag cttgtgcgtg
    36661 gaaacctgcg ggaaccgggc gattagctcc cgcgcatcga gcaggatcac cgcgcccggc
    36721 gcgccgccaa agtccaccgc gtcgagaaag atcacgcgct caaagccccc gttcgccacc
    36781 cgccccaggc agcgctcggg agcggagccg gcaatcatca cctccgccgt gatgggcgag
    36841 gcttgcagcg cttccgccag ccgcaccccg aagccatcgt ccccatagtc cacattcccg
    36901 atccccacca ggcagacgcg gccctggagg agcggtgcga gcaattggcg caggtcgggc
    36961 atcagatatc ctctcccagt ttcttgattt gctcgtaggt gaacaccggg ccgtccacgc
    37021 acacgtaggc cacgccgatg cagcagtggc cgcatttgcc gacgccgcac ttcatatacc
    37081 gttcgagcgt cgaaacgatg tccttttcct gaaaccccat cgccagcagg tccttgatta
    37141 cgaagcggaa catgatgggc gggccgcaga caaaggcgat ggtgttctcc accggaatct
    37201 tcacccccgg ccgctggaac aggctgccga ccacacccac ggggccgtcc cagccctccg
    37261 ccgcgcgatc cacggtgaga tggcattcga cgcccgccga ttgctgccac tcgcgcaggt
    37321 tggggacata catcagctcc ttcggcgtcc gggcgccgta gaaaatctgg atgcgctgat
    37381 atttatcccg gtgccggagc gcgtacacga tgcaggagcg cagcggaatc agcccgatgc
    37441 ccccggccac aaacaccagg ttcttcccgt aataatcctc catggaaaac ccgttgccgt
    37501 aggggccgcg gacggcaaac cggtcgccgg gccgcaactg gtgcagcgcg gcggtgcaac
    37561 tgccgaccgt gcggatggtg aagaaggttt cctcctcggt cgggctgggg ggcagcgagg
    37621 ccggaaattc accgacgccg aacaacgaca cctgcgcgaa ctggccaggg cggaaccgtt
    37681 tgaactttgc ctgctccgcc ggatcctcaa aatgccagta gaaggtcttt acctccggga
    37741 tgtcgtccgt cacccggtcc agcaccgcga gcttgggcag atagatattt tcttccgccg
    37801 gttcgcagga agtcaccttg gggagggtcg ggttcatgag gggggaatcc ggtcgtggat
    37861 cgcggtggcc cgccggatca tctcgaccac gctgggcatg ccgacatctc cgtggcaaac
    37921 cgccgcgcag cggccgcagc ccacgcaggc catggtcaac tcgcgctgga tgaagtaatg
    37981 cgccagcttg cgcatgaagc ggcggttgcg gcggtcatgc accgcgcggc gcgggttgaa
    38041 gccgccggcc atgcgcgtga atccgctcag ggcgcaggcg tcccagatgc gcacccgctc
    38101 gatttcgtcg gggcccacct ggcgatcgct gacggtaaag caggtgcagg cggggcaaac
    38161 gtaggtgcag ccgccacatt ccaggcagcg attgccgatt tcctcccacg tctgctccag
    38221 gatgtcgccc cgggaaacgt agcgcaccgt ggccgcaaac caactggtgg tggtcttgaa
    38281 ccgtttgcgc acctccgcca ggatggcgcg gcgtttttcg acgtgcgccg ccgtggccgg
    38341 ccggaacatc ggctgggtga agaacgcctc gcccgccgcc gtgccggcaa caaccatgaa
    38401 ctcgtcgccc aggtccatga gctgcaggtc aaaatgatcg cgcgccgccg ggccggtgtc
    38461 ggcgcatacg cagaagcact cgtccccaat atcgcgctcc aggtccgtgc agaccacgtt
    38521 gatcaggagc aggttctcgc ggcgtttctg gtaatagaga tcgcgaaact cgcgcccaag
    38581 gtagaagcgg tccaggtgcc agatgccggc caggtcgcag gagcgaaggc cgaagatggc
    38641 gcgcttgcgc tcccggtaaa tcggctcgat gtggaaggtt ccatcgtgcc gccgcacctt
    38701 cagcaaccgc tcgatatgcg gcaggacaaa gcgtttgggc ggataatacg ggttgggcaa
    38761 atgaatctgg acctggccgc gattttcgtc ggtgaccagg tcgaaccacg tgtcgcgccc
    38821 gcgcccggca aagggggcaa atacgtcgta gccctgctgc cggaggagat ccaccagcgt
    38881 cagcacctgg gcgcgttcca gcaagcgggc tttcatgcgg gctccttcgc gatgcgcgcc
    38941 gggatgagct ggacgccgct ttgcgccgcg ccgcagattt gccgctgcac ctgccgcaca
    39001 aaatacggca catagaccgc cccctggcgc acctcggagg tgacccgggc gttgatcacc
    39061 gccgagccgc cggcggccgt cagccgcacc ttttccccgt cgcggatgcc ggcttgctgg
    39121 gcatcggcgg ggttgatttc gacgaagcct tccgggtaat cgagcagcag gatgcggtac
    39181 tcgcgcttca gtgtctcgct gtgtttgatc agcacgctct ggttccagta atagagcgaa
    39241 tgaccaaaga ccagcgtgag ggggtattcg tccgaaacca ccgccgccgg cgcatggcgg
    39301 gcaatcggcg caaacttgaa cccctgcgcc ggcaggccgt ccgcaaagag atagcgcgtg
    39361 ccgagcggcc ggtccttgct gcacggccat tgccggccgt attcggcggc caggttgcgg
    39421 tagttcgcgc cgccgtagaa gggaatgacc gcgccgatct cgtccatcac gtcggcggcc
    39481 gactcatatc gccagtcagc gcccagcgcg cgcgccacct gggtcaactg ccgccaggcg
    39541 ggggtggtgc cgggcagcgg atctataatc tgctccgcca gttgaatgcg gcgttcggtg
    39601 ttggtaaagg tcacctgctc ctcgccaaag gcggtggtgg gcaggaccac atgcgcgtgg
    39661 cgcgcggtgt ccgtgagaaa caaatgctgc gccacgacca gctcgcaccg gtcgagcgcg
    39721 tccgccgcgt cgccgaagaa ggcggagctc accgggtcgt agcggcagag ccagatcgcg
    39781 cgcaccgggc tggcgccgcg gttgttgaaa atggcgcgcg aggccaggcc gggtgcggcc
    39841 ggcaatttcg tttgccagac ccggcccacc tccgcgcgcg ccgccgcgct gtcaacctgg
    39901 cgatagcccg gcaaccggtc gggcagcatg cccatatcgc agaccccttg caggttgttg
    39961 tgctcggtca gcgcgaagat gcccgcgcct tcccgcccga tttgccccgt caacagcgcc
    40021 aggttcacaa tggcctgcac cggttcggcg tcgcgggcct cgatgccggt cgaatacaga
    40081 atcgccgccg accgggcccg ggcgtaagcc agggccgccg cctcaatcag ccccgcgtcc
    40141 acgccgcacg cctcggccgc cgccagcaaa tcgtaatccc gcacccgcgc cagaaaccgg
    40201 tcgtagtcgc ggcagtgggc cttgatgaac ggcaggttca tcaggccgcg gtccacaatg
    40261 accttggcca gggcgccgta gaggatcggc tccgtgccgg gcttgagttg caggaaaagg
    40321 tccgcgcttt ccgccacccg gtggcgccgg ctgtcaatga ccacgagtgt ggcccccgcc
    40381 agcttggccc ggatgacggt tccgccgatg ctgggcaact ggagagccag gtccacgccg
    40441 tccacgatga tgacctcgct gcgggccagt tcgccgatgg aattggtcgt cgccggcacg
    40501 ccgagcatgt cgagcaggac attgatggaa ttgttgcagt aaaccccggt gccgtggtcc
    40561 acgttgttgg ttccgatgac cgcccgggcc agcttttgca gcaggtacga ttcctcgttg
    40621 gagcagcgcg gcgagttgag aaaagcgaag gcctccgcgc cgtgccgcgc gtgcagctcc
    40681 ttcatgcggc cgatgatgaa accgagcgcc tcctcccacg acacttcctc gaactggccg
    40741 cccttcttga gcagcgggcg tttcaaccgg ccgggggaac tggccacctc gtgcacatgc
    40801 cagcctcgca cgcaaatccg gccctcgttc gccggatgcg acatgctggg ataggccccc
    40861 acgatctggt tgccaaccgc ctccagataa atcccgcagc cgaccccgca gaaggtgcaa
    40921 gtggtcagtt tatgcgccat gaaggttgct tagttcgggg cggcgggggt cttggcgggt
    40981 gtcgtcgcca tcaaattccg cgttcacgga tgatccggtt gtcttccccg agcacaatga
    41041 cgcggggttt ccaggtctgg gcggcggccc attccacctc ggtaaagctt aaaatcgtga
    41101 tcaggtcgcc cggtttgccg agtcgggcgg tggcgccgtt gaggatgatc tggcccgagc
    41161 ccggctcggc ggctatggcg taggtctcaa atcgttcgcc attggccatg ttgccgcaga
    41221 gaatgcgctc gtacggcaac aaccccactt tatccatgag gtcgcgggca atacccaggc
    41281 tgccctcata atttacgtcg cccgccgtca cccgcgcgcg atgtattttc gactttaata
    41341 aatgcaccag cataagctcg ccgccgcacc ggggaacccc ggttggcgtc atttgccaac
    41401 tatagggttt ggcattccgg atgtaaatcc gctttccagg gaattgcctc gccgatggcc
    41461 ggcacgccgg gccggctact ggcgctcgta ctcgcctttg ccgacacgct tcagcaccgt
    41521 gaacccggct ttcttggcgt cggaaatcga gaccgtcctg ggggagttaa aggtggacca
    41581 aacccggcga accggttttt tacacgcggg acattgggtc aggggcgccg cggacagcgg
    41641 gcgtcgcagg gtaaacttgc cgccacacac cacgcaatca ccctcgcaga gttcatattc
    41701 atacaatggc atcatggctc gtcttctata ctctaattca tgttccaaac tttgccggtc
    41761 gaagtcaagc gcgcccgccg ccgttcacta cctatccact gccaggccct cggcgggaac
    41821 cgccaactcg ggaagggcgt gcggttgggc tgcgcaacgt caacgtttca gataaagcat
    41881 tcgcgaagcc aaaagccatc cggatgccca tccgcccttg aaaaaccgca tttagtcagc
    41941 cctggtttca taactagctg tttttgcgga gcttaataat tatcttccca tccgctttcc
    42001 tcttctcgcc ctttccagcc ccccgaattc gttatccaga ttccctggaa acaccctgcc
    42061 ttcaggccgt gtacaagggg tgtacaaggg atgctcaccg ggtgtaccag ctaaaatcct
    42121 atggactcct gatgggcacc ccttgtaca
//
LOCUS       DAOPQG010000014        41946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1224_length_41946_cov_15.674441, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000014 DAOPQG010000000
VERSION     DAOPQG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 41946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41946
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1224_length_41946_cov_15.674441"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            667..1074
                     /locus_tag="PKI07_02710"
     CDS             667..1074
                     /locus_tag="PKI07_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49993.1"
                     /translation="MDKPPKISEVPVSVAPIEVLPPQPAVRPLAASLEGPPIHALSAL
                     ILVAVDMLWGLFDWMPADWIVAIPSCFAAVFVPTFLIQRQLKQDSRGRALTFAVILGV
                     LAAIPTFVTGTPVGLALLAWTGLGKLLGKPPLR"
     gene            complement(1097..1471)
                     /locus_tag="PKI07_02715"
     CDS             complement(1097..1471)
                     /locus_tag="PKI07_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49994.1"
                     /translation="MLKKFLPVLLLPLLLAGCVTQLTNLTPLQQKRSTNNLYTVEVAL
                     HTRQQTLRWESIEPQIIADQGTYQMRATPLVSNRWEGLIPVPPGVHSVEYRYKLNYQY
                     NRMGNPGNGSYLSPKYTLQILD"
     gene            complement(1596..2075)
                     /locus_tag="PKI07_02720"
     CDS             complement(1596..2075)
                     /locus_tag="PKI07_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOC49995.1"
                     /translation="MATLPQLIEEDIARLDAELRELLERSDATTALIIDKGGFLITSQ
                     GDAQQFDLTTVAALASGAFMANQTIAGLVHETNFNSIYQQGEQFSMLVTCVDENCLLL
                     VVFKAQVSVGAVKYFAAPAVKRIALQLQIAQERAPDAGLDLSVLNLADPSQVFKRKA"
     gene            complement(2077..2691)
                     /locus_tag="PKI07_02725"
     CDS             complement(2077..2691)
                     /locus_tag="PKI07_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HOC49996.1"
                     /translation="MAIINQASKELQVKIVYYGPAMGGKTTNLVQVHDHVQTAAGNKG
                     KLVSLATSSDRTLFFDFLPIEAMAIKGFKTKFQLYTVPGQVIYNTTRQLVLRGVDGIV
                     FVADSQYEKMAENVESFANLEENLRTLRLNLADIPYVLQYNKRDLPNVAPVEYLEFLL
                     NNRELQVPAFSASAHKCDGVFEALNMITRMLLHKFISQGTPQYA"
     gene            complement(2696..4342)
                     /locus_tag="PKI07_02730"
     CDS             complement(2696..4342)
                     /locus_tag="PKI07_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOC49997.1"
                     /translation="MKSLVRRLVDNSTPAPPKPSPTPAPAATRPAATPFRSEPASPRH
                     NGAHHQNGRGIELPLKPILAGLPLELQARVINVDVSIVTIMIPLEKILSQLSRGVVQI
                     SFGELRQAAPGLFTMENDRDRVMVPLPLGEILSRLNPALISCRRVQSQVQVPEDVLSP
                     FDDRNQSRTLVIGPAKPPAAAPAAAAPAAAPPPVVLPPARPAAEPAPEPIIPPARANI
                     TFAPTPAPPAAPSPIPLSARHFTRPTQEPPSPLAPDPAPAPAAAAPEESGSLLVNVSS
                     LAEAWPEAIRRELLLLNLAEARVALPVESVELALKQGRIVFSWKTLRAWLRPAPPPAA
                     SIHDATVLELPLKVVAPLFLARQKEASPGQKKVWVDSAIPNLFFGGTPPPAGGPPPAA
                     PAPPKPTDTNYYVWDDTSDTVRAEGEEGKSGPSPGTKFVAKYATPNEVVSRAASLEGV
                     AGALVALPDGLMVASRLAPEVNGDTLAAFLPQIFAKISQCTRELRMGELNNVNFTVGN
                     IPWKIFRVNAIFFAAFGSAREPLPTAQLAALAAELDHKAK"
     gene            4911..5972
                     /locus_tag="PKI07_02735"
     CDS             4911..5972
                     /locus_tag="PKI07_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC49998.1"
                     /translation="MERRPRILLADDDPDLLELYREMLAQLPGQPEIHTAVGGARALT
                     MLETEPYQVLICDLKMPKLDGLQVLAIVRRKYPDLRTVALTAEVDEQFRSRAYALGVD
                     LFWTKPATEHETRLFLECVASLLGRETEVGFRGVQTRSLMDIVQMECLSRSSAVLRIT
                     NGALGGKIWIQDGELVDAEAPGLEGEAAFHRILSWKAGNFEMLPAEPSRPRQIYKPYN
                     ALLLESAQALDEAHAGLAAADAPPPPPSPLAQVSRVEGVEFVLTLPADAPGEHAARGL
                     ENPERMAAWTRQSLERFRSLGDHLGAGALDRIEGFGPQRHVALTTQGATEFCVGWKNT
                     LAPDEVREQMQKVLALWAS"
     gene            5960..6283
                     /locus_tag="PKI07_02740"
     CDS             5960..6283
                     /locus_tag="PKI07_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC49999.1"
                     /translation="MGLLNLFAKSAPTLLRLPTGSFTVDREGKVLSGTLPSSFPAPVV
                     RDIARQVLATFSEAAAAQLPLAELIIHYPSLKITARELRGGAIVFLAPKAPASPMSRG
                     NSLLL"
     gene            6280..6687
                     /locus_tag="PKI07_02745"
     CDS             6280..6687
                     /locus_tag="PKI07_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50000.1"
                     /translation="MSDQKLDQLVDQVENYIECWKQFNSYVNLAHNKKFNPEDENQFL
                     EIKSVLIQQLELILSVVEVSSPTREEIHALITDVPSLRFLSELNEGARRNLESQWHRI
                     YVGWHALLGQLKVRQRSETGKSGISAVFGKKKK"
     gene            6770..7582
                     /locus_tag="PKI07_02750"
     CDS             6770..7582
                     /locus_tag="PKI07_02750"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOC50001.1"
                     /translation="MPDWIAVVLLGIVEGITEFLPISSTGHLLIAEHWLHIRQSDLFN
                     IVIQSGAVLAVIPLFHRRLHQLFFRWREPAAQDYAAKLALAFVLTGGLGFLLDRGGFQ
                     LPEQLLPVAWALLIGGVGFVGVETWLRGRPLNDQVTWTMAVAVGLGQVLAAIFPGASR
                     SGTTILLCLLLGLARPAATEFCFLVGIPTMLAAGGLKLFKALHHPPPGTPPERWDMVL
                     LGFLVSAVVSFIAVKWLLRYVQTHTFTAFGWYRMGLAILIALGLLAGWGTHP"
     assembly_gap    7700..7799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7800..8107)
                     /locus_tag="PKI07_02755"
     CDS             complement(<7800..8107)
                     /locus_tag="PKI07_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50002.1"
                     /translation="MKRISFLLLAALALGAPSALRAQDAATEERLNKLSGRLDDLAAA
                     QEAFRAQIADLSREIQSLREQVARPSASYARPEDLNRLAEAVKEVDRKRMNDADKTQA
                     "
     gene            complement(8181..9314)
                     /locus_tag="PKI07_02760"
     CDS             complement(8181..9314)
                     /locus_tag="PKI07_02760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC50003.1"
                     /translation="MSNDLQIVPLSRRPREVLRFLKVSYGIYEGDPNWVAPLLMDLKK
                     VFTDANPLFEHAVMQLWVATRGGRDVGRIAGIIDRNHNRLAAEPAAFFGFFESVDDPA
                     VSHGLFAAVADWARQAGLPRLLGPMNPTTNDECGLLVEGFDSPPVFMMPYNPRYYVAF
                     MEKAGWRKAKDLLAFYMDLAKIPMERLSRIAEKIRQRNPELRFRPVRRKTLATDLAKV
                     KEVYNAAWEDNWGFVPMTDAEIDFMAARLSPLLMEGLVWLAEAGNEPVGFLLALPDYN
                     LALQPLRGRLLTPRVLGFLPYVWGWKCPPRTRVITLGVKEKYRARGLESAMLIEGLKV
                     GMAAGVTESEASWILEDNVAMCRVLEAIGGRVYKTYRLYERAA"
     gene            complement(9584..10294)
                     /locus_tag="PKI07_02765"
     CDS             complement(9584..10294)
                     /locus_tag="PKI07_02765"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOC50004.1"
                     /translation="MAPAASKCINRPAQERLSCERELWRKGLRFVAGVDEAGRGPLAG
                     PVVAAAVVLPCAWAEAGLDPRLHALNDSKQLTEAQREEYYALLTAHPDIRFAIASVAV
                     EVIDRINILQATHRAMNQALAQLQPPPEYALVDGRPVKSLALPHAALVKGDARSYSIA
                     GASVLAKVTRDRLMREWDRRYPGYGFAEHKGYGTPQHLAAIQALGPCPIHRRSFAPFR
                     SAEMELPLTLPPETPPGD"
     gene            complement(10266..11198)
                     /locus_tag="PKI07_02770"
     CDS             complement(10266..11198)
                     /locus_tag="PKI07_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOC50005.1"
                     /translation="MARKLSSIAPSWWDYTTLDAGLVRDAAALTPQKMLKLSRPGFKV
                     VMYDTLEDFYLAEALEYITAWKQSTLDNPVGVCGPIGPTEQLPLVARLVNELNLNLYS
                     AHFWGMDEWFIDGKALPATHPLSFEKADRELCFNRIRKDLVMPDANLHFPNANIAAFR
                     KTWNSGIRCAVMQGGQGDVKHWAFNDPPRRTGKYRNQPPSPAEYRKLATRVVDLHPLT
                     IAQNARTSGGGNISLVPTQAITVGPVETWLAEKVSIWQAGTHDNPFGQRLTCLMISQR
                     IPDAAVPMSLLADHPNVQFNYYRQGLGSCRVEMH"
     gene            complement(11240..12043)
                     /locus_tag="PKI07_02775"
     CDS             complement(11240..12043)
                     /locus_tag="PKI07_02775"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC50006.1"
                     /translation="MRNKTAIAIGAHPDDIEFSMAGTLLMLKRAGYQIHYLTVANGDC
                     GSTEYPNAILRSIRRAEARAAAQVLGAHFHPSITNDLEILYNLEQLRALAAVIREVKP
                     GIILTHSPQDYMEDHTNTSRLVVSAAFTRGMPNFKSVPARPTADYEVTIYHALPHGFC
                     DQLRRRLIPGAFVNTAPVHKIQMQALAEHKSQQKWLDVSQGLNSFLLAVEDMSRAVGR
                     MSRKFKYAEGWRRHLHYGFSATDADPLAEALGANYRVNRIYEQLCQRGY"
     gene            complement(12033..13079)
                     /locus_tag="PKI07_02780"
     CDS             complement(12033..13079)
                     /locus_tag="PKI07_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50007.1"
                     /translation="MKKYNVGIVGYGWVATAHIPAINATALGQVTAVCSSRKLDGAAL
                     SAQYGGPIRVYNDLDEMLANPEIHVVSLCGFPSQRLDQVRRAARAGKHLILEKPLCLS
                     LKELRAMHQAVKRARVKTCVCFECRYANQFQVIKTVLDNGLLGQVHYGEVDYYHGIGP
                     WYAQFRWCRSKAEGGSSLLEAGCHALDALLMCMGSEVDEVMSYTSKSRNRLFAPYEYP
                     STSVTILKFKNGAAGKCTSSIDCLQPYYFHTHLVGSKGSLLDNKFHSTDLGTNKAAWS
                     TLAMKPVDSGDVSDHPYQAQFTAFFDALARGREMPLTSLDDAVVSHEVVLAADCAAEL
                     GRPVKMDHLSKYAK"
     gene            13223..13753
                     /locus_tag="PKI07_02785"
     CDS             13223..13753
                     /locus_tag="PKI07_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50008.1"
                     /translation="MKIEQLHIGLRVRHPQYGEGVVKGISEATAEIQFGAGRRTVAPE
                     PSGLEPAEAQACISGLDLPLAQVVRQTVEATIQRLGLENPEAVVAQLGARWHRGKVVL
                     HPADPALQTKEVPLETFFHKIVMIRNNLRVLEQKINAHPQLTDGEKVELQQYLTRSYG
                     SLTTFNLLFKDKEDAF"
     gene            13819..14667
                     /locus_tag="PKI07_02790"
     CDS             13819..14667
                     /locus_tag="PKI07_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC50009.1"
                     /translation="MSTTNTSLSDRTAVCSWSLQPASPQDLIAKLQAAGVRRVQLALD
                     PLREAPGVWGETAALFRQNGVTIVSGMFGCVGEDYTTLQTIRDTGGIAPDATWEQNWT
                     NIRATAALARELGLKLVTFHAGFLPHDEQDPGFAKMLRRLSETADAFQTAQLALGLET
                     GQETAPALARLLQQLKRPNVGVNFDPANMILYDKGDPIAALQTLGPWLRQVHIKDARR
                     TRVPGTWGEEVVVGTGEVNWPAFFTTLERLNFKGDFVIEREAGNQRVADVRTAREVIA
                     RAGRAS"
     gene            complement(14833..16287)
                     /locus_tag="PKI07_02795"
     CDS             complement(14833..16287)
                     /locus_tag="PKI07_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016402647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOC50010.1"
                     /translation="MQIPRILTSLLGGGALALLLTPWPAPAGFTLPEMSAQTKACAEC
                     HRKETSAIYAQWGASKHYRANIGCYECHMAVTNDLDAFEHYGQTISTMVTPKDCGRCH
                     ELEVGEFNASHHAKAARILGSLDNVLAEVVEGNRGFKTPGFPNGTSAAAVSGCWQCHG
                     VEVKINANGKPDPAVWPNTGIGRINPDGSEGSCAACHARHEFSAAQARTPDTCGKCHM
                     GPDHPQIEIFNESKHGIAYRAHVDKMKLDNPKWIVGEDYSSAPTCSTCHMSATRNQPV
                     THDVGLRISWNNRPELSIRPEISDAKLGLATKDIPWQTRRTNMVDVCLNCHNQLFVDS
                     FYQQYDAIIELYHEKFAKPGLALYQAAQPLLRPAQFSNPLDFTWYEIWHHEGRRARHG
                     ASMMGPDYTHWHGTYEVAKHFYSKYVPELQELVEKHANSTDAAKKEAAAKLSKLLEET
                     LNHPNHQWYQNRMDPAEKARRDQERQEFQKRYAK"
     gene            complement(16299..17528)
                     /gene="nrfD"
                     /locus_tag="PKI07_02800"
     CDS             complement(16299..17528)
                     /gene="nrfD"
                     /locus_tag="PKI07_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOC50011.1"
                     /translation="MKDYLTFLWRAGRVSFQGGAKFYLWMTLLTVLVLVGLHAWARQF
                     VLGLETTGLTNPVSWGAYIANFTFLVGVAAAAVMLVIPAYIYKKEFMHEVVIFGELMA
                     IAVIVMCLLFVAVDLGHPDRFHHMLPPFGIFNWPDSILSWDVIVLNGYLLLNLHIAGY
                     LLYCRYRQRPPTRRFYIPFVFISIAWAVSIHTVTAFLYVGLAGRSYWHHPLVPSRFLA
                     SAFVAGPGLMILTFQIIRRVTRYYIGDQPIFTLRMIMTVAMIINMFLLGSELFTEFYS
                     PTMHAAAARYLYFGLHGHQGLVPWIWTAIVLDSIGLLLLITPLSRKIGWLNIACVTCF
                     VGVWIEKGMGLIVPGFVPSPLGQIVEYLPTLNETLICLGVWALGALLFSWMVRVAIPI
                     MNGSLRAHPQLNPATKT"
     gene            complement(17525..18499)
                     /locus_tag="PKI07_02805"
     CDS             complement(17525..18499)
                     /locus_tag="PKI07_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC50012.1"
                     /translation="MNSDPQNEVSPQTLERVYQPLTRRSFLKGAAGAAAGLVGLGAAL
                     SPLLELERGELTLDELLQKRYLELTPEELQRILKALEDKCERERGVRPTLLDRKPLPG
                     VEFGYALNLTRCIGCRKCAYACLKENNQSRESADDIEMSYIRVLELEKGAINLETSSL
                     YYHPATVPEPGKYYMPVQCQQCRQSPCTKVCPVRATWPEPDGVIVVDYNWCIGCRYCM
                     AACPYDARRFNYKKPTLPPAEINPRQNYLSNRLRRQGVVEKCTFCLQRSRAGKYPACL
                     EVCPTGARVFGNLNDPQSEIRYVLDHKRVYILKEEVGTLPRFYYFFDK"
     gene            complement(18496..19263)
                     /locus_tag="PKI07_02810"
     CDS             complement(18496..19263)
                     /locus_tag="PKI07_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50013.1"
                     /translation="MSPIKKKAVRAAVVIALCAAAATVWFAGGADPFAATPPAPAHLP
                     TVARPPAPQPALDAAGDAYLDTTPPRRMVGLDLIRNGWTYNCMECHRLFPAKWHYDRP
                     MAEHQDIRLEHGNNRFCLNCHHPTNRNAFVDYDGGEIAAADVALLCGKCHGTIYRDWE
                     AGVHGRVSGFWKADLGGKTRLRCIQCHDPHHPKFEAMKPLPPLRYPARGAHPPVHRAA
                     EPDARVAPARPEARGPETGAAPAAAAAAGLRRVETVS"
     gene            complement(19452..20726)
                     /locus_tag="PKI07_02815"
     CDS             complement(19452..20726)
                     /locus_tag="PKI07_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50014.1"
                     /translation="MRFLTRPRIQWAGGGLAAWLALAAPAPGRAAAALDVVRTTKAAP
                     AGFVEIGGVEAGAFNPAIWQAGTLHWVPDLRSPLLAPRSGKFRNIYAPSAVETPGGYR
                     LFYGAWDGLPTGNDCLYSATTDANFQKFSHRRAIIVPGGYVHVCNVNALGLDGGGFEL
                     LATVYPVRERNKPGFFRSDATGTNWNGGRGEPYTVRARDIIAMDGYAYPNADINGMNV
                     LLREDGARRLYFGDFRGGGGTFRATSRDGRNYTFEAKVLAGPGLVNDVKKFRVGADCY
                     YLMGLHENRRRLWQTISTNGLAFPPARELLAHLDAADAYIVALGWVTRGAQESPGRRL
                     LGVLYGAGPVSALDQNRIYARWLQKRVDFVARDGTRISGTRALGPGQQLLDFAGSPAR
                     RGRFELYAEDGVTRLGVSPEFDVQSGQVFHLR"
     gene            complement(20809..22095)
                     /locus_tag="PKI07_02820"
     CDS             complement(20809..22095)
                     /locus_tag="PKI07_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50015.1"
                     /translation="MNKPCQFTRRDFLRTSLVASAAISLVPRHVLGGPGVTPPSEILT
                     KAVIGVGSMGRAHLSSINPTAKLLAVCDVDAKHLQAALSKCGPEVKGYRDFREVLARK
                     DIDIVHIPTPPHWHALISIAAAKAGKDIWCEKPLSRTIGEGEAVKAAMAKYRRIFRLN
                     TWFRFEGAFYGVGVPVRLIKKAVQNGLFGWPLKVTLGGPTGFDWKQNEWCGRTDLTPQ
                     PVPPELDYDFWLGPAPKKPYHPHRVHGTFRGYWDYDGGGLGDMGQHYLDPVQYLLDKD
                     NESPVRIEADTDPQDPDAVQPWRRISIRYADGCEIIFDGENKDKEAAFIAGPEGKLFR
                     DFESDIPNLRAKIQSLPDPEPQLTDFIQAVKTRQKFALNEVNGHRSCTLVNLAKIAVQ
                     TGRVLHFDPKKQRFIHDQQANALIHQPMRKPWKLRV"
     gene            complement(22618..23394)
                     /locus_tag="PKI07_02825"
     CDS             complement(22618..23394)
                     /locus_tag="PKI07_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50016.1"
                     /translation="MDSNPILKSFPWTTTILAATVVILAALALFAYLKTMRSVRETVD
                     QAGRTAAEVARNFQTGRITQTFRESIPQITATHGDVLELAVFRAEETFKRTDERRIGW
                     GWVYLGTTVAEIRVPVSFRYHLRLSDPWRLAARGQLCVVLAPPIRPSLPPAIHTDEME
                     RRAESGWARFDKNEQLEALQRSLTPALEQRAGDTNHLALVREACRHSVAEFVKKWLLH
                     EKQWQPDRFTSIVVLFPDEADAATDTDLLRFRHEPTLRLK"
     gene            23809..24642
                     /locus_tag="PKI07_02830"
     CDS             23809..24642
                     /locus_tag="PKI07_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50017.1"
                     /translation="MKNLRKKIGAFTLIELLVVIAIIAILAALLLPALARAKSRALRV
                     SCTNNLKQVGLAMRTFAIDNDGAMPMGLAGAMGGASEDVETFARVLGTTQLGSHGAAK
                     IFLACSNELSTPKVLFCPAEYETSVRQAATSFADSTGVSSTTVSQIPYTNDLNISYFV
                     GVDATETQPQMFLTGDHNLGGNANPPTVAYLAAPATGTPKISLGTNFTANMGPAWMDN
                     MHSKQGNVGMADGSVQGFNRARLQEALKNSSDTGGRTSTKFPLANGASAGLNCNRIML
                     P"
     gene            complement(24958..25866)
                     /locus_tag="PKI07_02835"
     CDS             complement(24958..25866)
                     /locus_tag="PKI07_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50018.1"
                     /translation="MKSLDVTVAQINCRRWRRRGFTLIELLVVIAIIAILAALLLPTL
                     GRAKNTAKGIANLNNLRQIGIANQMYLDDSNGFFAHHEGYYYQGAIIDRQEGKNLGLT
                     KAVKTHWPDQMVCYAPQPKVFLSPFLTTEQIASEDFSRPFGIDGYRQYIYGGYGYNLH
                     YLGYATGEHGKPGYSARLGADIRHPSSTVFVGDTAGSKSHRGVYTGKPEAPYALDPPL
                     PSLNLGAKLTGYYEGDDGMIETPSPGPYDWQVRAYPAPRNNGKPGFVFVDTHAEGMGI
                     QKIDDSNGDGVRDNGNWNGLHDPFNR"
     gene            26447..>27614
                     /locus_tag="PKI07_02840"
     CDS             26447..>27614
                     /locus_tag="PKI07_02840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOC50019.1"
                     /translation="MLASCVVRWWLALTVAFSAVTVLAGPNELYDPPALFLTWKRDPA
                     RTMIIHWQTVGEARAEAYYRASGAGDWIKVQGESHPFPKSKRTIHTVELTGLTPRSDY
                     EFCFLPGEKTFRFRTLPATLVSPVRFVNGGDAYHERNWMDTMNRLAGKFDPAFVVIGG
                     DLAYSCGGKSPERMERWDDYFDSWKTNARASDGRLVPLLVTIGNHEVVGSWDQSPTNA
                     QGFYALFPLPGERGYACLDFGNYLSLFLLDSSITHPIEGAQTDWLRHELAKRRTMPHI
                     IPVYHIPAYPSIRSDATGQSSEHTQMIRRSWCPLFEEAGVTVAFEHHDHSNKRTYPIR
                     DGKIDPTGIVYLGDGAWGVHLRQPDKTKPRWYIARSDGIRHLYVVTLYPESCHVL"
     assembly_gap    27615..27714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(27780..28544)
                     /locus_tag="PKI07_02845"
     CDS             complement(27780..28544)
                     /locus_tag="PKI07_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50020.1"
                     /translation="MTRRPAFPRRARFVLLLAGLLAGWPLRAAPSPEALFQAGVSAYR
                     AADYARAAEAFRQSAARQPAAGTLLNLGNAEWQRRQTGAAILAWEQAQWLHPFDPAAR
                     QNLRYARKAAQLEAPELAWYEVISTWLPVAWWAWLAGGSFWMAISAAMLPGVFRRRKA
                     TWHQAVAAFGFMVFLLCVPAYIGVQTRSRLGFILPPNTPLRLTPTHEAQTVTRLAAGE
                     PARCLRTRGRFVLIRASRTLGWVEQAHLGRICPASD"
     gene            complement(28541..>28928)
                     /locus_tag="PKI07_02850"
     CDS             complement(28541..>28928)
                     /locus_tag="PKI07_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50021.1"
                     /translation="WDRRRRYQEAHPDVVRRRRARRALRRQRRLLRRAARAADAPRFA
                     AAAVNALRTACAPHYPAEPRALVGGDVLPLLPATGPSDRAGEVVRRFFAATDAARFSP
                     NPASVAELLSLQPELEQVLAALEEKL"
     assembly_gap    28929..29028
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29029..30269)
                     /locus_tag="PKI07_02855"
     CDS             complement(<29029..30269)
                     /locus_tag="PKI07_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50022.1"
                     /translation="MKLKRQVRLPGGGGERGRKMLSLLAGGLIAAAAPLAAQTNAAPS
                     RDPLMSLMLSQPRLEIPALTTATAEFDPPVVRPGEPSFLRVTFNALEDSVEWPATIAG
                     PPGLELRPGAHGQMLKVSGVNFEPYTAFNTRAQAARPGTFVVPEFVVRINGQPVTVPS
                     ARLEVAATVPASVPPRLPLTLELPRTNLFVGQAVMARMVLAGRPGNAIHGLAQPQLTG
                     PGFLVDGTTSRQRVEAVTQNGGSVLSFIYETTFIPLTAGRLTVQAQGFMASSRLSPPA
                     ASPGATVIPPPIVQYTLIESDPLELNVRPLPREGELPGFTGAIGHFRIGPPQLATNAL
                     RVGEPVNLTVTLTNYGDAPLARVTPPPPPQTRDWQVFATADFAPVQPIGPPPPSAPPP
                     AGAPPPAPELHGVLTFSYILIP"
     gene            complement(30318..31310)
                     /locus_tag="PKI07_02860"
     CDS             complement(30318..31310)
                     /locus_tag="PKI07_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50023.1"
                     /translation="MMARLLISLWGFAAPVLLAGALAAGDAPEAISPPAPPPLPSQEP
                     EAPATPEPPPPASPREFFNAGTRQLHSGKLREAEAFLEMALTRQKDSLQPHALYNLGL
                     TRFAQGVEQGKKGPAPQPTLARGQAAAGETDLALRQANAALADDDVQRMVAAYLRGWG
                     ARRELKAATAAVRQALKVYGAVLQRWERAAGDFKSAFELNPAAADARHNAEVTDRHIA
                     RLVDSLNELQQCLSGLCGKESQLSAAMKKLKGRIPAADMPPGVAGEEEDDEEQTMGPQ
                     PGQQEGPSREGEEMHLSPEQAGWLLEAYKLDTERRLPMGQESTSEPRDRSRPAW"
     gene            complement(31314..32342)
                     /locus_tag="PKI07_02865"
     CDS             complement(31314..32342)
                     /locus_tag="PKI07_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC50024.1"
                     /translation="MDFSHPHFAEPQWLWLALAAPLLLAALQGYSARARRRQLARMAA
                     PEFLAALTRSHSPARYLLKNVLLLLAVAGVGLALARPQWGEQTEISHLYGQDTLFILD
                     CSRSMLASDLAPSRLQRTKLALRDYVQHRGRGRVGLVACAGQAFLQCPLTYDYAAFQD
                     ALAAVDDQAIPVPGTDLGRALDEASRALAKSRPPRLCVLLTDGEDLERGGIRAAQTLA
                     KQGLIVFTIGVGTAAGAEIQFTNEHGQRETLRDRHGEVVRSRLDEATLRAIAQATGGA
                     YFPLGPLGEGLARVQLALADLQTGPASAPARKLGVDRFHWPVAGVLALLILESLIGTR
                     RRLRDINA"
     gene            complement(32342..33382)
                     /locus_tag="PKI07_02870"
     CDS             complement(32342..33382)
                     /locus_tag="PKI07_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC50025.1"
                     /translation="MTGNLEFQYPWLLGLLALLPIYSFLRGRAGRLAALRFPSADIAR
                     AAGAAARSAAGRLLLFLRLLAAALCIVALAGPRFANDHTEMQASGVDIMLVLDLSWSM
                     MALDMGAPSERISRFDIAAEVIEDFIRKRTNDRLGLIVFSAVPYLASPLTLNHDWLIE
                     NLRRLHVGLIRDLGTAIGDAAAAAVKRLKNLKDAKSRIIILLTDGDNNKGEIDPVPAA
                     QLAAALGVKVYTIGIGIEQPCLLPLFDPSTGKLELDAHGNLRPGMTLQPANYAVLGKM
                     AALSGAKFYRATNRRELRRIYDEIDRLEKTEIKLRRFTTYRPLYQWPLLAAFGLLALE
                     LTLAHTRLRRVP"
     gene            complement(33387..34046)
                     /locus_tag="PKI07_02875"
     CDS             complement(33387..34046)
                     /locus_tag="PKI07_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 family protein"
                     /protein_id="HOC50026.1"
                     /translation="MRTPAGRRQHARRGGKPDLHAGSFRLLLPAAFLARAAAGIPELD
                     DIPPLRPPRAELPPTFWEQSGLWVIIAGFLLLALAGAAIWFLTRPRPPQAVPPEAQAR
                     QALEPLCHQPEDGALLSRVSQVLRHYVQAAFALPPEEMTTAEFCRAVAGHAAIGPELS
                     TALAEFLRACDQYKFSPPAPAPPLDAVSRALQLVEQAEARRAPPHPDGATPAAAGQPA
                     A"
     gene            complement(34043..34960)
                     /locus_tag="PKI07_02880"
     CDS             complement(34043..34960)
                     /locus_tag="PKI07_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOC50027.1"
                     /translation="MTLSELLENVRRVEVRTNRLVNDTMVGAYLSHFKGRGMDFEELR
                     EYIPGDEVRDIDWNVTYRMGRPFVKRYREERELAMVLAVDISASSTFGSQHRTKRQFA
                     AEIAATLAISAARSSDKVALLLFSDRVELYLPPRKGRRHLLRLIREMLLFKPRQRGTS
                     IPGALAFLNRVLPRRSIVFLLTDFLHSFAPPAAPPGAGRDAVQEIGLTNARHDLVCVH
                     LHDPRESQLPPAGLLTLEDAETGELLELDSSRPAVRERFAHTNAARLAELDRALRRAG
                     VDTLRFSTAEPFAQALQTFFETRRGRRRG"
     gene            complement(34957..35940)
                     /locus_tag="PKI07_02885"
     CDS             complement(34957..35940)
                     /locus_tag="PKI07_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOC50028.1"
                     /translation="MTNTPLNGQVQAATGWIGRLRAEVARVIVGQEQLVDRLLVGLLA
                     NGHVLLEGVPGLAKTLCVRTLASAIQATFHRIQFTPDLLPADIIGTLIYNPQDGQYRA
                     TKGPVFANFVLADEINRAPAKVQSALLETMQERQVTLGGETMPLPAPFLVLATENPID
                     QEGTYPLPEAQVDRFMFKVLLDYPSFAEERKILDRMAFTAPETQVQPVIPLAEILQTR
                     KLVDQIHVDEKVRDYVVHLVFATRKPDAYQLDLKHFIQFGASPRATIYLTLAARAWAL
                     LQGRAYVTPEDVKSIGPDVLRHRILLSYEAEAQAVTTDAIIRKIFNAVPVP"
     gene            complement(36076..36525)
                     /locus_tag="PKI07_02890"
     CDS             complement(36076..36525)
                     /locus_tag="PKI07_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOC50029.1"
                     /translation="MPAEPQATLQKLLDLLGFDATVEEHHLEEGLLLDVKTEDSGRLI
                     GRQGQCLADLQYITNRLLFQQDPSVPKVMVDISGYRAQAREALVKKAQAAAEKVRRWG
                     DVVELEPLNAFDRRIVHQALKDDPRVETQSVEVEGSDKKAILLRPKS"
     gene            complement(36587..38401)
                     /gene="yidC"
                     /locus_tag="PKI07_02895"
     CDS             complement(36587..38401)
                     /gene="yidC"
                     /locus_tag="PKI07_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOC50030.1"
                     /translation="MDRKSILILVVCFILLMLWHPLVNKLYPPKPLPPGATNAVQSPL
                     APTNPAEAAPSAPPVISEAPAPAPRAWAAATEPERLLILTNNDARYTFTSHGGGLKQV
                     ELIHYPETVSSRRTKPAPTNNLATLNAFTPTPALAILGADALSSDGVFTLTQTGDGVR
                     AEKTLTNGLRLVKEFQLSTNYLLTATVRLENRSPQPLTLPPQEWVVGTATPLGADDKG
                     LSLGVIWYNGAKTFDQAGASYFSTRGFACMPRVPPAEYRAGADNVVWAAAHNQFFALA
                     VMPQQPAGQIVVRKVLLPPPPAEEGRFGAVASPPSEGYEGALVYPGQTLDAGQVVTNQ
                     IILFAGPKEYRTLARIAARFGNNLDLVMGYSGFFGFFAKGLLLAMNGLHSALGLSYGW
                     AIIAITIIIKLLFWPLTQASTRSMKRMQALQPQMTAIREKYKDDPAKMNKKTMEFMKE
                     HKVSPLGGCLPMLLQIPVFFGFFRMIQSAIELRGARFLWIKDLSQPDTLFVIPGTHFP
                     FNLLPLIMGATMLWQARLTPPSPGMDPMQAKMMRYMPLMFLVFLYNFSAGLTLYWTVQ
                     NLLTILQTKLTRTLPEPAAAAPVPAKAPALTPSPKKKK"
     gene            complement(38416..38763)
                     /gene="yidD"
                     /locus_tag="PKI07_02900"
     CDS             complement(38416..38763)
                     /gene="yidD"
                     /locus_tag="PKI07_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOC50031.1"
                     /translation="MNLAQRILIFGVRLYRWTLSPAKTFLFGPLGRCRFTPTCSEYAL
                     EALRHHGAGAGSWLAMKRIARCHPWGGCGFDPVPEGKPAARHRNSAETSRAENRQGSP
                     GLPFHPERQSAHS"
     gene            complement(38760..39149)
                     /gene="rnpA"
                     /locus_tag="PKI07_02905"
     CDS             complement(38760..39149)
                     /gene="rnpA"
                     /locus_tag="PKI07_02905"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOC50032.1"
                     /translation="MTIPPPQRLRFGRAARLKRGADFSRLRQAGERQVSGCLIANWQR
                     RPAEAASRLGVITGSRIGGAVVRNRARRLLREAFRLHQHDLATPVDLVLVARASITGK
                     GFAEVERDYLAALRKAGLLSPARRPNA"
     gene            complement(39165..39305)
                     /gene="rpmH"
                     /locus_tag="PKI07_02910"
     CDS             complement(39165..39305)
                     /gene="rpmH"
                     /locus_tag="PKI07_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOC50033.1"
                     /translation="MMKRQYQPSKVRRRRQHGFLARNASKSGRGILRNRRRVGRKRLT
                     PV"
     gene            39643..41046
                     /locus_tag="PKI07_02915"
     CDS             39643..41046
                     /locus_tag="PKI07_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOC50034.1"
                     /translation="MKTWGRLLCWTAIAAAAFHGAYASAHTSILVVLYLYALLRLARA
                     DRWRQAFYSGLAAGFLIAVARLAFFWRLFSAGAIGLWLIYAFWVGLFVALARLCLRRF
                     GAPGGWLLIPFVWTGLEYFRSELYYLRFSWLNIGYAFAGAPWQAGLKLTGVYGAGFLL
                     AGLAAAAAGARRKSRLRAGAVLVLGAGAVCLGGWLAGKVSPAPSVAGIRVAGVQMEFP
                     TEAEVVLRLEDLVQEHPEAELLVLSEYTFDGQVPAAVRAWCRENRRYLVVGGKDPAPG
                     GNYDNTAFVVGPDGDICFRQVKAVPIQFIKDGRPAREQKPWASPWGKLGLCICYDLSY
                     TRVTDRLIRQGAQALIVPTMDTADWGRAQHVLHARVTPVRALESGVPIFRLASSGISQ
                     LTDGAGRVLATAPFPGGGATLAGTLELRAAGRLPLDRGLAPCAVAVTGALLIGFVFLR
                     RPSVRQPPAQENCGMVA"
BASE COUNT         6991 a        12911 c        14281 g         7463 t
ORIGIN      
        1 ccgccactcc ttgggctgga agtccgcccc gaggtaatgc gggaaggctg gcgggcggag
       61 ggtatttgcg caagggcaat tttttgggat tggtgatggc aggccggtgc ggagcgccgc
      121 gtgggggcac gcggcctaca gggggcggga gggtgcgcgg gggcctgtgg gttgccgggg
      181 gcgcggtgct gcgagagggc atgggggctg ccgcggcgtg cttccatttc ctgcaggccg
      241 cgtgggggca ggcggcgctc gggggccgtt tgtctggcgg ttgagaaaaa catttgcgcg
      301 cccgcccggg ttgtctgagg gaaagcccgc cggcttggga gggcaatctc atccccgcta
      361 tcctcaaccg cccaggcccg ggacgcacgg ccaggctccc gctccgaagc gcttaacaat
      421 ggcgggccgg cgataagctc gggttaattc ccttgtttta gctgttaaag gccgggaaga
      481 attggcgccg gccggagttg cggctgctgc ggttgcggat tcggcctgga aggttgggtt
      541 ttcggaggga agcggcttgt ggggggagcg gcaatcggtc ttgccgtctt gggggctggg
      601 ccgcgccggg gaaatactgc gctcaaaacc tcgacaaggc gtcagattaa gccatctttg
      661 gggagcgtgg acaaaccgcc gaaaatcagc gaggtgccgg tcagcgtggc gccgatcgaa
      721 gttttgccgc cgcaaccggc cgtccggcct ctggcggcgt cgctggaggg gccgccgatt
      781 catgccctgt cggcattgat tttagtggcg gtggatatgt tgtggggatt gtttgattgg
      841 atgccggcgg actggattgt ggctattccg tcctgtttcg cggcggtttt tgtgccgacg
      901 ttcctgattc agcggcagct caagcaggat tcccggggcc gggcgctgac gtttgccgtg
      961 attttgggtg tgctggccgc catccccacg tttgtcaccg gcacgccggt tggcctggcg
     1021 ctgctggcct ggacggggct gggcaaactg ctcggcaagc cgccgctgcg ctagcccggg
     1081 ggcgggcgcg gggcggttaa tcgaggattt gcagggtgta tttcggggag aggtagctgc
     1141 cgttgcccgg gttgcccatc cggttgtatt gatagttcag cttgtagcgg tattcgacgc
     1201 tgtggacgcc cgggggcacg ggaatcaggc cttcccagcg attgctgacg agcggggtgg
     1261 cccgcatttg gtaggtgccc tggtcggcga tgatttgcgg ctcgatgctc tcccagcgga
     1321 gcgtttgctg gcgggtgtgc aaggccacct cgacggtgta aagattgttg gtgctgcgtt
     1381 tttgctgcaa gggcgtgagg ttcgtgagct gggtgacgca tccggccagc agcagcggta
     1441 ggagcaatac cggcagaaat ttctttaaca taggcgcagc ttgacaaacc ggcgcccgcc
     1501 tgtaaagccg ctttttcccg ccgtttcccg gcgggccggg cggggaggcg gggcgggcgt
     1561 ccgcgcgtgc gcggggcgcg ggttgcgcgg cgggctcagg ctttgcgctt aaagacctgg
     1621 ctgggatcgg cgaggttcag gaccgagagg tccagcccgg cgtcgggcgc gcgttcctgg
     1681 gcgatttgga gctggagcgc gatgcgtttg acggccgggg cggcgaagta tttgacggcg
     1741 ccgacgctga cctgggcctt gaacaccacg agcaggaggc agttttcatc cacgcaggtg
     1801 acgagcatgc tgaattgctc gccttgctga tagatgctgt tgaagttggt ttcgtggacc
     1861 agcccggcga tggtctggtt ggccatgaag gcgccggaag ccagggcggc cacggtggtg
     1921 aggtcgaact gctgcgcgtc cccctggctg gtgatgagaa aaccgccttt gtcaatgatc
     1981 aacgcggtcg ttgcgtcgct gcgttcgagc agctcgcgca actcggcgtc gaggcgggcg
     2041 atgtcttcct cgatgagttg ggggagggta gccatgtcat gcgtattgcg gggttccctg
     2101 gctgatgaat ttatgcagca acatgcgggt gatcatgttg agcgcttcga agacgccgtc
     2161 gcatttgtgg gcgctggcgc tgaaggcggg cacctggagt tcgcggttgt tgagcaggaa
     2221 ttccaggtat tcgaccgggg ccacgttggg gaggtcgcgc ttgttgtatt ggaggacgta
     2281 ggggatatcg gcgaggttga gccgcagcgt gcggaggttt tcctccaggt tggcgaagct
     2341 ttccacgttc tcggccatct tttcgtattg ggaatcggcg acaaagacga tgccgtccac
     2401 accgcggagc acgagctggc gggtggtgtt gtagatgacc tgccccggga cggtatagag
     2461 ctggaatttg gttttgaagc ctttgatggc catggcttcg atgggcagga aatcgaagaa
     2521 caaggtgcgg tcggagctgg tcgccaggga gaccagcttg cccttgttgc cggcggcggt
     2581 ttggacgtgg tcgtgcacct ggaccagatt ggtcgtcttg ccgcccatgg cgggtccgta
     2641 atagacgatt ttgacctgca gttctttgct tgcttgattg ataatggcca taagattatt
     2701 tggctttgtg gtcgagttcc gcggcgaggg ccgccagttg cgcggtgggc agcggctcgc
     2761 gcgcgctgcc gaacgcggca aaaaagatgg cgttcacccg gaagattttc cacgggatat
     2821 taccgacggt gaaattgacg ttgtttagtt cgcccatgcg cagctcccgg gtgcattggc
     2881 tgatcttggc gaagatctgc ggcaggaacg ccgcgagggt gtcgccgttg acttcgggcg
     2941 ccagccggct ggccaccatc aggccgtccg gcagggcgac gagcgccccg gccaccccct
     3001 ccagcgacgc ggcgcgggag acgacttcgt tgggcgtggc gtatttggcc acgaacttgg
     3061 tccccggcga gggtccgctc ttgccctcct cgccctcggc ccgcacggtg tcggaggtgt
     3121 cgtcccagac atagtaattc gtatcggtgg gtttgggcgg cgcgggggcg gccgggggcg
     3181 ggccgccggc gggcggcggg gtgccgccaa agaacaggtt gggaatggcg ctgtccaccc
     3241 aaaccttctt ctgccccggg ctcgcttctt tctggcgcgc caggaacagc ggcgcgacaa
     3301 ccttcagcgg cagttccagc acggtggcgt cgtggatgga ggccgcgggc ggcggcgccg
     3361 ggcgcagcca ggcgcgcagg gttttccagg agaaaacgat gcggccctgc ttgagggcga
     3421 gttccacgct ctcgaccggc agcgcgaccc gggcctctgc caggttcagc aacagaagct
     3481 cccggcggat ggcttccggc caggcttcgg ccagggagga tacgttgacc aacagggagc
     3541 cgctctcctc cggggcggcg gcggccggcg cgggcgcggg gtctggcgcg aggggcgacg
     3601 gcggttcctg cgtcgggcgg gtgaagtgtc gcgcggaaag aggaatgggc gacggcgccg
     3661 ccggcggcgc gggcgtgggg gcaaaggtga tattggcgcg ggcgggcgga atgatcggct
     3721 ccggcgccgg ttcggcagcg ggacgcgccg gcggcaggac caccggcggt ggcgcggcgg
     3781 ccggcgcggc ggcagcgggc gcggcggcgg gcggcttggc cgggccgatc accagggtgc
     3841 ggctttggtt gcggtcgtcg aaggggctga ggacgtcttc cggcacctgg acctgcgact
     3901 gcacgcggcg gcaggagatc agggcgggat tgagccggga caggatctcg cccaggggca
     3961 gcggcaccat tacgcgatcc cggtcgttct ccatggtgaa gaggccgggc gcggcctggc
     4021 ggagttcgcc aaaggagatt tgcaccaccc cgcgggagag ctgggagaga atcttctcca
     4081 gcgggatcat aatggtgacg atgctgacgt ccacgttgat gacccgggcc tgcagttcca
     4141 ggggcaggcc ggccaaaata ggctttaagg gcagctcaat tccacgtcca ttttgatgat
     4201 gcgcgccgtt gtggcggggc gaagcgggct cggagcggaa cggggtggcg gccgggcggg
     4261 tggcggccgg ggccggggtg ggagacggct tcggcggtgc cggggtggag ttgtccacca
     4321 gcctccggac caggctcttc aaaggacgta tgatattaaa gttcatgcaa tagtcagtgc
     4381 gcgtgcatct acgcagtcat ttcaaccccg gcgcggctcg gcgaccctta atggcgcgcg
     4441 ccatgaccgg gcgccggccg gaggccgggt cgggccaggc cgcggggcac cttgacggtt
     4501 cgtttggcgc acgattcatc gtctgtgttg cggttgaact gattctggcg gcgttgggtg
     4561 caggatttgt gccacgaccg gcccggagcg tgaataaggc cgccgatgtc aaccaaaaga
     4621 ggattgcggg tcaaagccgc gcgccatttc ggggccagcg acgggcgttc ttgtcttatc
     4681 cgtggacgaa atgtcctatt gccgtccgcc tcctccggcc ggaaaaggct cggggaacgg
     4741 gttgacggac ggcaaagcgg gtcaatccgc ccgcgaatgt cgcgcaattc gacaaatctt
     4801 cacgtgccgg gcgaaagggg aataggcctt gctatggtag cggagagaac gactggcttt
     4861 ccggcttgag tttgcgcgga gaacaagata atagtgaaca caggtagctg atggaaaggc
     4921 gccccaggat tttattggcg gatgacgatc cggatttgct ggaactgtac cgggagatgt
     4981 tggcacagtt gcccgggcag ccggagattc acaccgcggt cggcggggcg cgcgcgctga
     5041 cgatgctgga gaccgagccg taccaggtgc tgatttgcga cctgaagatg ccgaagctgg
     5101 acgggttgca ggtgctggcg attgtgcggc gcaaataccc ggacctgcgc accgtcgccc
     5161 tgacggccga agtggatgag caatttcgct cccgggctta tgcgttgggg gttgatctct
     5221 tctggaccaa gcccgccacc gaacacgaga cccggctgtt tctggaatgc gtcgcgtcgt
     5281 tgctgggccg cgaaacggag gttggcttcc gcggcgtgca aacccggagc ctgatggaca
     5341 tcgtccagat ggaatgcctt tcgcgcagtt cggcggtgct gcgcattacc aacggcgccc
     5401 tgggcggaaa gatttggatt caggacggcg aactggtgga cgccgaggcc cccggcctgg
     5461 agggggaggc cgcctttcac cgaatcctgt cctggaaagc gggcaacttt gaaatgctcc
     5521 ccgccgagcc cagccgcccc cgccagattt acaagcccta caacgcgctg ctcctcgaat
     5581 ccgcgcaggc cctggacgaa gcgcacgccg gcctggcggc cgccgatgcg ccgccgcccc
     5641 cgccctcgcc gctggcgcag gtgtcccggg tggagggcgt cgagtttgtg ctgaccctgc
     5701 ccgctgacgc gcccggggag catgccgcgc gcggcctgga gaatccggag cgcatggcgg
     5761 cctggacgcg gcagagcctg gagcggttcc gctcgctggg cgatcacctg ggcgcgggag
     5821 ctttggatcg gattgaaggc tttggcccgc aacggcacgt tgccctgaca acccaggggg
     5881 caacggagtt ttgcgttggc tggaagaata ccctggcgcc ggatgaagtc cgggagcaga
     5941 tgcagaaggt gttggcgtta tgggcctcct gaaccttttt gccaaatcgg cgccgacgct
     6001 gctccggctg cccacgggca gtttcacggt ggatcgcgag ggcaaggtgc tctcgggcac
     6061 gctgccgtcg agctttcccg ccccggtggt tcgggacatc gcccggcagg tgctcgccac
     6121 ctttagcgag gctgccgcgg cccagttgcc cctggcagag ctgattattc actacccgag
     6181 cctgaaaatc accgcccgcg aactgcgcgg cggcgccatc gtcttcctgg ctcccaaagc
     6241 ccccgcctca cccatgagcc gcggcaactc cctcctccta tgagcgatca aaaattggac
     6301 caactcgttg accaggtaga gaactacatc gaatgctgga agcagttcaa cagctacgtc
     6361 aacctggcgc acaataaaaa attcaacccg gaagacgaga accagtttct ggagatcaaa
     6421 agcgtcctga tccagcaact ggagttgatc ctgtcggtgg tggaggtgtc ttccccgacc
     6481 cgggaggaaa tccatgcctt gatcaccgac gtgccctcgc tgcggttcct gagcgagctg
     6541 aacgagggtg cccggcgcaa tctcgaaagc cagtggcatc ggatttacgt cggctggcac
     6601 gccctgctcg gccagctcaa agtccgccag cggagcgaaa ccggcaagtc cggcatcagc
     6661 gcggtcttcg gcaagaagaa gaaatagccg ggcggcgctg tggttcgcgc gccgggggag
     6721 gccgcatttg gggcttgtgt ttggcgcccg tttatcgtct aaccatccga tgccagactg
     6781 gatagccgtt gttttgctcg ggatcgttga ggggattacg gagtttctgc ccatttcctc
     6841 caccggccac ctgctcattg ccgagcactg gctgcatatt cggcagagcg atctcttcaa
     6901 catcgtcatc caatccggcg cggtgctggc ggtgattccg ctctttcacc gccgcctgca
     6961 ccagttgttc ttccgctggc gcgagccggc ggcgcaggat tacgccgcca aactggcgct
     7021 ggccttcgtg ctcacgggcg gcctggggtt cctgctggat cggggagggt tccagttgcc
     7081 ggagcagctt ctcccggtcg cctgggcgtt gctgattggc ggcgtcggtt ttgtgggggt
     7141 cgaaacctgg ctgcgcggcc gccccctgaa tgaccaggtc acctggacca tggccgttgc
     7201 ggtggggctg gggcaggtcc tcgcggcgat ttttccgggc gcctcgcgct ccggcacgac
     7261 catcctgctt tgcctgctgc tggggctggc gcggcccgcg gccacggagt tttgctttct
     7321 tgtcggcatt cccaccatgc tggcggccgg cggcctgaag ctctttaagg cgctgcatca
     7381 ccctcccccc ggcacaccgc cggagcgctg ggatatggtg ttgttgggct tcctggtttc
     7441 cgcggtcgtc tcttttattg cggtgaaatg gctgctgcgc tatgtgcaga cgcatacgtt
     7501 caccgccttc ggctggtacc ggatgggcct cgccattctg atcgccctcg ggctcctggc
     7561 cggctgggga acgcatccgt gaacgcgagg gcgcacggca ggcggtaagg gcgcggggaa
     7621 acgtgccccc gactggcgcg gcgagttctc tcccgcggga gccgcggcta cggcgcggga
     7681 atgaatatct tctgcccgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     7801 ctgcctgcgt cttatccgcg tcgttcatcc gcttccggtc cacctctttc accgcctccg
     7861 ccaggcgatt caagtcttcc ggccgggcat agctggcgct gggccgggct acttgctcgc
     7921 gcaagctttg gatctcccgg gagaggtcgg cgatttgcgc ccggaaagcc tcctgggccg
     7981 ccgccaaatc atccagccgg ccgctcaatt tgttgagccg ttcttcggtc gccgcgtcct
     8041 gcgcccgcaa tgcggagggg gcgccgagcg ccagtgccgc caacaacaga aaggaaattc
     8101 gtttcatgcg ccgacaatag aaatcccatc cgccgagttc aaggcccaaa ccgcacccgg
     8161 acggaggccg ccgccgcggt tcaggcggcc cgctcgtaga gccggtaagt cttatacacc
     8221 cgcccgccga tggcctccaa tacgcggcac atggcgacgt tatcctccag aatccacgac
     8281 gcctcggatt cggtgacgcc tgcggccatc cccaccttga gcccctcgat cagcatcgcc
     8341 gactccaatc cccgggcccg gtatttctcc ttcacgccca gcgtgattac gcgggttcgg
     8401 ggtgggcatt tccagcccca cacgtaggga agaaatccca gcacccgcgg tgtgagcaat
     8461 cgcccccgca gtggctggag cgcgagattg taatccggca gggccagcag aaacccgacc
     8521 ggctcattgc ccgcctcggc cagccaaacc agcccctcca tgagcagcgg cgagaggcgc
     8581 gccgccatga aatctatttc ggcatccgtc atcggcacaa acccccagtt atcttcccag
     8641 gccgcgttat agacctcctt taccttggcc aggtcggtcg ccaaggtctt gcgccggacc
     8701 ggccggaaac gcagttccgg gttgcgctgc ctgatcttct cggcaatgcg gctcagccgc
     8761 tccatgggga tttttgccag gtccatgtag aacgccagca agtccttggc cttgcgccag
     8821 cccgcctttt ccatgaacgc cacatagtag cgcgggttgt agggcatcat gaacaccggc
     8881 ggcgaatcaa acccctcgac caacaaacca cactcgtcgt tggtggtggg gttcatcggc
     8941 cccagcaagc gcggcaagcc cgcctgccgc gcccagtctg ccaccgccgc aaacaatccg
     9001 tggctcacgg cggggtcatc caccgactcg aagaagccga aaaaggccgc gggttccgcg
     9061 gcgagccggt tgtgattgcg atcaatgatg ccggcaatgc gccccacatc ccgccctccc
     9121 cgcgtcgcca cccaaagctg cattacggca tgttcgaaca gcgggttggc atccgtgaac
     9181 acctttttca agtccatcag cagcggcgcg acccagttcg ggtcgccctc gtagatgccg
     9241 tagctgacct tcagaaaacg cagcacttcc cgcgggcggc gggacaacgg aacaatctgg
     9301 aggtcgttgc tcatgcctgg ctggatgcgg ctaagccggg acggcgccgc tggccacggg
     9361 aagaatgaag gaactcaatg ccccggtcaa tccggcaacc ccgcgctttg ggagagtcta
     9421 tcaaaaccgt ccatgcccct ggctggcggc tccgcgccat cctggaagaa gggcttttcc
     9481 cggccccaaa ccgcccggct ccgccgggaa tccaatcgag tgccccagcc gcttcgcggt
     9541 tgctcaaacg ttctttggac gtcccgcccc gggccggggc tggtcagtcg ccgggaggag
     9601 tttctggcgg gagcgttaac ggcagttcca tttccgccga ccggaacggg gcgaagctgc
     9661 ggcggtggat cgggcagggg ccaagggcct ggatggccgc caggtgctgc ggggtgccgt
     9721 agcctttatg ttcggcaaag ccatagccgg gatagcggcg gtcccactcc cgcatgaggc
     9781 ggtcgcgcgt gaccttggcc agcacgctgg cgccggcgat cgagtagctg cgcgcatcgc
     9841 ctttgaccag cgcggcgtgc ggcagggcca gggatttcac cggccggcca tccaccagcg
     9901 catattcggg cggcggttgc agttgcgcga gcgcctggtt cattgcgcgg tgggtcgcct
     9961 gaaggatgtt gatgcggtca atgacctcca cagccacgct ggcgatcgca aaacggatgt
    10021 cggggtgggc ggtcaggagg gcgtaatact cctctcgctg cgcttcggtc agttgcttgg
    10081 aatcattcag cgcgtggagc cggggatcca gccccgcttc ggcccaggca cagggcagca
    10141 cgaccgccgc ggcgacgacc ggccccgcca gcgggccgcg gcccgcttca tccacgccgg
    10201 cgacgaaccg gaggcccttc cgccacagtt cgcgctcgca ggacagccgt tcctgagccg
    10261 ggcggttaat gcatttcgac gcggcaggag ccaaggccct ggcggtagta attgaactgc
    10321 acattcgggt ggtccgccag cagggacatg ggcacggcgg catccggaat tcgctgggag
    10381 atcatgaggc aggtcagccg ctggccgaag ggattgtcgt gggtgccggc ctgccagatg
    10441 gacaccttct ccgccagcca ggtttcgacc gggccgaccg tgatggcctg ggtgggcacc
    10501 agggagatgt tgcccccgcc ggaggtgcgc gcgttctggg caatggtcag cgggtgcaaa
    10561 tccaccacgc gggtggccag cttgcggtat tcagccgggg agggcggctg attccggtat
    10621 ttgccggtcc ggcgcggcgg atcgttgaag gcccaatgtt tcacgtcccc ctgcccgccc
    10681 tgcatgacgg cgcagcggat gccggagttc caggttttgc ggaaggcggc gatgttggcg
    10741 ttcgggaaat gcaggttcgc gtccggcatg accaggtctt tgcgaatgcg gttgaagcac
    10801 agctcgcggt cggccttttc aaaggaaagc gggtgggtgg caggcagagc cttgccgtca
    10861 atgaaccact catccatgcc ccagaaatgg gcggaataga gattcaggtt caactcgttc
    10921 accaggcgcg ccaccagggg cagttgctcg gtggggccga tagggccgca gacgccgacc
    10981 gggttgtcga gggtggattg tttccaggcc gtgatgtatt cgagcgcctc ggccaggtaa
    11041 aagtcctcca gcgtgtcata catcacgacc ttgaagccgg gccgggacag tttgagcatc
    11101 ttctggggcg tgagcgcggc cgcgtcgcgg acgaggccgg cgtcgagggt ggtgtaatcc
    11161 caccaactgg gagcgatgga gctgagtttg cgtgccataa gtcgtattgg ttttcgtgtt
    11221 tgaggttaag tccagcggtt caatagccac gttgacagag ttgctcgtaa atccggttca
    11281 cccggtagtt cgcgcccagc gcctccgcca gtgggtcggc gtccgtcgcg ctgaacccgt
    11341 aatgcagatg ccggcgccag ccctcggcat acttgaactt ccgggacatg cgcccgacgg
    11401 cgcgggacat atcctccacc gccagcagaa acgagttcag gccctggctc acgtccagcc
    11461 atttctgctg gcttttatgc tccgccagcg cctgcatttg gattttatgc accggcgcgg
    11521 tgttgacaaa ggctcccggg atcagccgcc gccgcaactg atcgcaaaaa ccatgcggca
    11581 gcgcgtggta aatcgtcact tcgtagtccg ccgtcgggcg ggccgggacg ctcttgaaat
    11641 tgggcatccc gcgcgtgaag gccgccgaca ccaccagccg cgaagtgttg gtgtgatcct
    11701 ccatgtagtc ctgcggcgag tgggtcagga tgatgccggg tttgacctcg cggatgacgg
    11761 ctgccagggc gcggagttgc tccaggttat acaggatctc caggtcattc gtaatgctgg
    11821 ggtggaagtg agcgccgagg acctgggccg cggcgcgggc ttcggcgcgg cgaatggagc
    11881 gcaggatggc gttggggtat tcggtgctgc cgcaatcgcc gttggccacc gtcaggtaat
    11941 gaatctggta gcccgcccgc ttgagcatca gcagggtccc ggccatggaa aactcgatgt
    12001 cgtccgggtg cgccccgatg gcgatggcgg ttttatttcg catacttgct caggtggtcc
    12061 atcttcaccg ggcgccccag ttccgccgcg caatccgcgg ccagaaccac ctcgtggctg
    12121 acgaccgcgt cgtccaggct ggtgagcggc atctcccggc cccgcgccag cgcgtcaaag
    12181 aacgcggtaa actgcgcctg gtaagggtga tccgatacgt cgcccgaatc caccggcttc
    12241 atggccaggg tgctccaggc ggccttgttg gtgcccaggt cggtggaatg gaatttgttg
    12301 tcgagcaagc tgcccttgct gccgacgagg tgggtgtgga aatagtacgg ctggaggcaa
    12361 tcaatcgagg atgtgcactt gcccgcggcg ccgttcttga acttgagaat cgtcacgctc
    12421 gtgctgggat actcataagg cgcgaacagc cggttgcggg atttggaagt gtagctcatg
    12481 acctcgtcca cctcgctgcc catgcacatc agcagcgcgt ccagggcgtg gcagccggct
    12541 tccagcagcg aactgccgcc ctcggccttg ctccggcacc agcggaactg ggcataccac
    12601 gggccaatgc cgtgatagta atccacctcg ccgtaatgca cctgccccag cagcccgtta
    12661 tccaggacgg ttttgatgac ctggaactgg ttggcatagc ggcactcgaa gcaaacgcac
    12721 gtcttgaccc gggcccgctt cacggcctga tgcatggcgc ggagttcctt cagcgagagg
    12781 caaagcggct tttccaggat gagatgtttg cccgcccgcg cggcgcgccg gacctggtcc
    12841 agccgctgcg agggaaaacc acagagcgac accacgtgta tctccggatt ggccagcatt
    12901 tcgtccaggt cattatagac gcggataggc ccgccgtatt gcgcgctgag agccgcgccg
    12961 tccagtttgc gggacgagca aaccgcggtc acctgcccca gggcggtggc gttgatggcg
    13021 ggaatgtgcg cggtggccac ccagccataa ccgacaatgc ctacattata cttcttcata
    13081 gcgataaaat tcgattcagt tgcgacgtaa cgtcaaccgg gatggcaccc ccttttgcgc
    13141 gctttgtcaa gcgattggtg gaaaagaaaa gcgggggact tcgccattcc cctgccgcgc
    13201 gggttcggct accctgcgcg gcatgaagat cgaacaactg catatcggcc tgcgcgtccg
    13261 gcatccccaa tacggggaag gcgtggtgaa gggcatttcc gaggccaccg cggaaatcca
    13321 gttcggcgcc gggcggcgca cggtggcgcc ggaacccagc ggcctggagc cggccgaggc
    13381 gcaggcctgc atcagcggcc tggatctgcc gctggcccag gtcgtgcgcc agaccgtgga
    13441 ggcgacgatc cagcggctgg gcctggaaaa ccccgaggcg gtcgtcgccc aactgggcgc
    13501 ccgctggcac cggggcaagg tcgtgctgca tcccgccgat cccgcgctgc aaaccaagga
    13561 agtgccgctg gaaaccttct tccacaaaat cgtcatgatc cgcaacaacc tgcgcgtgct
    13621 ggagcagaag atcaacgccc acccgcagct caccgacggc gaaaaagtcg agctgcagca
    13681 atacctcacc cgctcctatg gctcgctcac cacgttcaac ctgctgttca aggataagga
    13741 agacgccttc taatcaggcc ggcggcgttg ctttaagctt gtcttgacgg ggcgccaact
    13801 ggtaactggt cattcaacat gagcactacg aatacttcgc tttcggatcg caccgcggtc
    13861 tgcagttggt cgctgcaacc cgccagcccg caagacctga tcgccaagct gcaagccgcc
    13921 ggtgtgcggc gcgtgcaact ggcgctggat ccgctgcgcg aagcgcccgg cgtctggggc
    13981 gagaccgccg ccttattccg ccaaaatggc gtgaccatcg tctccggcat gttcggctgc
    14041 gtgggcgagg attataccac gctgcaaacg attcgggaca cgggcggcat cgcgcccgat
    14101 gcgacgtggg aacagaactg gaccaacatc cgcgccaccg ccgccctggc gcgggagctc
    14161 ggcctgaaac tggtcacctt ccatgccggc ttcctgccgc acgacgaaca ggatcccggc
    14221 ttcgccaaga tgctgcggcg gctgagcgag acagcggacg cgttccagac ggcgcagctc
    14281 gcgctggggc tggaaaccgg gcaggaaacc gcccccgccc tggcccggct cctccagcag
    14341 ctcaaacgcc ccaacgtcgg cgtgaacttt gaccccgcca acatgatcct ctacgacaag
    14401 ggcgatccca tcgcggcgct ccagaccctc ggcccgtggc tgcgccaggt ccatatcaaa
    14461 gacgcgcggc ggacccgggt gccgggcacc tggggtgagg aagtcgtcgt cggcaccggc
    14521 gaagtgaatt ggcccgcttt cttcaccacc ctcgaacggc tcaacttcaa gggtgacttc
    14581 gtaatcgaac gcgaagcggg caaccagcgc gtagccgatg tccgcaccgc ccgggaagtc
    14641 atcgcccggg ccggccgcgc cagttaagcc cgcggccgcc aggcttcagc gccgcccccc
    14701 gaagggggca gccattccgc cagcccgcgc cgggcgcgcg gggcgcgctt caccgcagcc
    14761 cggaaagcgc ccgcccgcga gcggcccgcg aacggcgggc gcccgccggg aatgtcccgg
    14821 gccgcgcccg aatcacttcg cgtagcgttt ctgaaactcc tgccgctcct ggtcgcgccg
    14881 ggccttttcc gccgggtcca tgcggttctg gtaccactgg tgattgggat ggttgagggt
    14941 ttcctccagc agcttgctca acttggcggc ggcttccttt ttcgcggcgt ccgtggaatt
    15001 ggcatgcttt tcgaccagct cctgcagttc gggcacgtat ttcgagtaga aatgtttggc
    15061 gacctcgtag gtgccgtgcc agtgggtgta atcggggccc atcatcgagg cgccgtggcg
    15121 ggcgcggcgg ccttcatgat gccagatttc ataccacgtg aagtccagcg gattgctgaa
    15181 ctgcgcgggc cggagcagcg gctgggccgc ctggtaaagc gcgaggcccg gcttggcgaa
    15241 cttctcatga tacagctcga tgatggcgtc gtattgctgg tagaacgagt ccacaaagag
    15301 ctggttatgg cagttgaggc agacgtccac catgttggtg cggcgggttt gccacggaat
    15361 atccttggtg gccaggccga gcttggcgtc ggagatttcc ggccgaatgg aaagctccgg
    15421 ccggttattc caggaaatgc gcaggcccac gtcgtgcgtg acgggctggt tgcgggtggc
    15481 gctcatgtgg caggtggagc atgtgggcgc ggacgagtaa tcctcgccca caatccattt
    15541 gggattgtcg agcttcatct tgtccacgtg ggcgcggtag gcaatgccgt gcttggactc
    15601 gttgaagatt tcaatttgcg ggtggtcggg ccccatgtgg cacttgccgc aggtgtccgg
    15661 ggtgcgcgcc tgggcggcgg aaaactcatg ccgcgcatgg cacgcggcgc aggagccctc
    15721 gctgccgtcg gggttgatgc ggccaatgcc ggtgttgggc cacaccgcgg ggtcgggctt
    15781 gccgttggcg ttgatcttca cctcgacgcc gtggcactgc cagcagccgc tgacggcggc
    15841 ggccgaagtg ccgttcggga atccgggcgt cttgaaaccg cggttgccct cgacgacctc
    15901 cgccagcacg ttgtccagcg agccgaggat gcgcgcggcc ttggcgtgat gcgaggcgtt
    15961 gaactcgccc acctccagct catggcagcg gccgcagtcc ttgggcgtga ccatcgtcga
    16021 gatggtctgg ccataatgct cgaaggcatc cagatcgttg gtgaccgcca tgtggcattc
    16081 gtagcagccg atgttggcgc ggtagtgctt gctcgcgccc cattgcgcgt agatggcgct
    16141 ggtttctttg cggtggcatt ccgcgcaggc cttggtttgc gccgacattt ccgggagggt
    16201 gaagccggcg ggcgccggcc acggggtgag cagcagcgcc agcgcgccgc cgccgagcag
    16261 ggaggtgagg atgcggggga tttgcataaa aggtgcgttc aggttttggt cgcggggttc
    16321 agttgcggat gcgcgcgcag gctgccgttc atgatcggga tggccacgcg caccatccag
    16381 gaaaagagca acgcccccag ggcccagacg cccaggcaga tcagcgtctc gttgagcgtg
    16441 ggcaggtatt ccacgatctg gccgaggggc gaggggacaa agccgggcac gatcaggccc
    16501 atgccttttt cgatccagac accgacgaag caggtcacgc aggcgatgtt cagccacccg
    16561 atcttccggc tcagcggcgt gatgagcagc agcaggccga tggaatccag cacaatggcc
    16621 gtccagatcc agggcaccag gccctgatgc ccgtgcaggc cgaaatacag ataacgcgcc
    16681 gcggcggcat gcatcgtggg agaataaaac tcggtaaaca gctcgctgcc aagcagaaac
    16741 atattgatga tcatcgccac ggtcatgatc atccgcaggg taaagatggg ctggtcgccg
    16801 atgtagtagc gcgtcacgcg gcggatgatc tggaaggtga ggatcatcag gcccggcccc
    16861 gccacgaacg ccgacgccag aaaccgcgag ggcaccagcg gatgatgcca gtagctccgg
    16921 cccgccaggc caacgtagag aaacgccgtg acggtatgga tgctcaccgc ccacgcgatg
    16981 gagatgaaca cgaagggaat atagaaccgc cgggtcggcg ggcgctgccg gtagcggcag
    17041 tagagcaggt agcccgcaat atgcaggttc agcagcaggt agccgttcag cacaatcacg
    17101 tcccacgaca ggatggagtc cggccagttg aagatgccaa acggggggag catgtgatgg
    17161 aaacgatccg ggtgccccag gtccaccgcg acgaacagca ggcacatgac aatcaccgcg
    17221 atggccatca attcgccgaa aatcaccacc tcgtgcatga actccttttt gtagatgtag
    17281 gcggggatga ccagcatcac cgccgccgcg gccacgccga cgaggaaggt gaagttggca
    17341 atataagcgc cccagctcac cgggttggtc aggccggtcg tttccagccc cagcacgaat
    17401 tgccgcgccc aggcgtgcag ccccaccagc accagcaccg tcagcagcgt catccacagg
    17461 tagaacttcg cgccgccctg gaaggagacc cgcccggcgc gccacaggaa tgtcaggtag
    17521 tccttcattt gtcgaagaag tagtaaaagc gcggcagcgt gccgacctct tccttgagga
    17581 tataaacccg cttgtggtcc agcacgtagc ggatctcgct ctgcgggtcg ttcaggttgc
    17641 caaagacgcg ggccccggtc gggcagacct ccaggcacgc gggatacttg cccgcgcgcg
    17701 agcgctgcag gcagaaggtg catttctcga ccacgccctg ccggcggagg cggttgctga
    17761 ggtagttctg ccgcgggttg atttccgccg gcggcagggt gggctttttg tagttgaacc
    17821 gccgcgcgtc gtaggggcag gcggccatgc aatagcggca cccgatgcac cagttatagt
    17881 ccaccacgat cacgccgtcc ggctccggcc aggtggcccg caccgggcaa accttggtgc
    17941 agggcgactg ccggcactgc tggcattgca ccggcatgta gtacttgccc ggctccggca
    18001 ccgtcgccgg atggtaatac agcgaggagg tttccaggtt gatcgccccc ttctccagct
    18061 ccagcacgcg gatgtaggac atctcgatgt catcggcgga ctcgcgggac tggttgtttt
    18121 ccttgaggca ggcgtaggcg cacttgcggc aaccgatgca gcgggtcagg ttcagcgcgt
    18181 agccaaactc caccccgggc agcggtttcc gatccagcag ggtcgggcgc acgccccgtt
    18241 cccgctcgca tttgtcctcc agcgccttga ggatgcgctg cagctcctcc ggcgtcagtt
    18301 cgaggtagcg tttctgcagg agttcgtcga gcgtcagctc gccgcgctcc aattccagca
    18361 gcgggctcag cgccgcgccc agtccgacca gccccgccgc cgcccccgcc gcgcctttca
    18421 ggaaggagcg gcgcgtcaac ggctggtaca cccgttcgag ggtctgcggg ctgacttcgt
    18481 tttgcggatc ggagttcatg acacggtttc aacgcgccgg aggccggcgg cggccgcggc
    18541 ggcgggcgcc gccccggttt ccggcccgcg cgcttccggc ctggcgggcg ccacccgcgc
    18601 gtccggctcc gcggcgcggt ggaccggcgg atgggctccg cgcgccgggt agcgcagcgg
    18661 cggcagcggt ttcatcgcct caaatttcgg atggtgcggg tcgtggcact ggatgcagcg
    18721 cagccgggtc ttgccgccca aatccgcctt ccaaaagccg ctcacccggc catgcacgcc
    18781 cgcctcccag tcgcggtaga tcgtgccgtg gcacttgccg cagagcagcg ccacgtccgc
    18841 cgcggcaatt tccccgccgt cgtagtccac gaaagcattg cggttggtcg ggtgatggca
    18901 attgaggcag aaacggttat tgccgtgctc gagccggatg tcctgatgct cggccatcgg
    18961 ccggtcatag tgccatttcg ccggaaagag ccggtgacat tccatgcaat tatacgtcca
    19021 cccgttccgg atcaaatcca gccccaccat ccgccgcggc ggcgtcgtgt cgagatacgc
    19081 atcccccgcc gcgtccagcg ccggctgcgg agcgggcggg cgggcgaccg tgggcaggtg
    19141 ggccggcgcg gggggcgtcg cggcaaacgg gtccgcgccc ccggcaaacc aaaccgtggc
    19201 ggcggcggcg cacaacgcga tcaccaccgc ggctcgaacg gcctttttct ttatcgggct
    19261 cattccgatt ttcggcaaaa ctctgcacaa aagcggggcc gcgcgcaatc ctaattctgc
    19321 gccggcgggc gtccccgcgc cggcccgctt tgcccggcgc cgccgccccc gttccctccg
    19381 cccccggcgg cgcgcgcgcc cgggcgccag cccggcgtcc ggcccgggcg ggtggcggac
    19441 cgcccgcccg ctcaccggag atggaaaact tgtccgctct ggacgtcgaa ctcgggggaa
    19501 acgcccagcc gcgtcacgcc gtcctcggca taaagctcga accggccgcg gcgggccgga
    19561 gagcccgcaa agtccaggag ctgctgcccg gggccgagcg cccgggttcc cgaaatccgg
    19621 gttccgtcgc gggccacaaa atccacgcgc ttttgcagcc accgcgcgta gatgcggttc
    19681 tggtccagcg ccgagaccgg cccggcgcca tacaggactc cgagcagccg ccgcccgggc
    19741 gactcctgcg ccccgcgcgt cacccagccc agcgccacga tataggcgtc ggccgcatcg
    19801 agatgggcca gcaactcgcg ggccggcgga aacgccaggc cattcgtcga gatcgtctgc
    19861 cacaaacgcc gccggttttc atgcagcccc atcaggtaat aacaatccgc gccgacccgg
    19921 aacttcttca cgtcgttcac caaccccggc ccggccagca ctttggcctc gaaagtgtaa
    19981 ttgcgaccgt cccggctcgt ggcccggaaa gtgccgcccc cgccccggaa atcgccaaag
    20041 taaagccgcc gcgcgccgtc ttcccgcagc aggacattca tgccattgat gtccgcgttg
    20101 gggtaggcgt agccgtccat cgcgatgata tcccgcgccc gcaccgtgta aggctcgccg
    20161 cggccgccgt tccaattcgt gccggtggca tcgctgcgga agaagcccgg cttgttccgt
    20221 tcccgcaccg ggtacaccgt ggccagcagc tcaaagccgc ccccgtcaag ccccagcgcg
    20281 ttcacgttgc acacatgcac gtagccgccc ggcacgataa tcgcccgccg gtgggaaaac
    20341 ttctggaaat tcgcgtccgt cgtggcgctg taaaggcagt cattgccggt cggcaggccg
    20401 tcccaggcgc cataaaaaag gcgatacccg ccgggcgttt ccaccgccga gggcgcgtag
    20461 atgttgcgaa acttcccgct ccgcggtgcc agcagcggcg agcgcaaatc cggcacccaa
    20521 tggagcgtgc ccgcctgcca gattgccggg ttgaatgccc ccgcctccac cccgccgatt
    20581 tccacaaaac ccgccggcgc ggctttcgtc gtccgcacca cgtccagcgc cgccgcggcc
    20641 cgccccggcg ccggcgccgc cagcgccagc caggccgcca atcccccgcc cgcccattga
    20701 atgcgcggcc gggtcaggaa tcgcatggcc ggcggctgct ggcgtccgcc ccgggcggct
    20761 tcgccgtttg cgtgcgcgcg ccaggcgccg ggcgcccgct cccggccgtt acacccgcag
    20821 cttccatggc ttgcgcatcg gctggtgaat cagggcgttg gcctgttggt catggatgaa
    20881 ccgctgcttt ttcgggtcaa aatgcagcac gcgcccggtc tgaaccgcaa tcttggccag
    20941 gttgaccagc gtgcaggagc gatggccgtt gacttcgttc aaggcgaact tctggcgcgt
    21001 cttcaccgcc tggatgaaat ccgtaagctg cggctccggg tcgggcaggg actggatttt
    21061 ggcgcgcaag ttcgggatgt cggactcaaa atcccggaac agcttgccct cgggcccggc
    21121 gatgaaggcc gcctccttgt ccttgttctc cccgtcaaag atgatttcgc acccatcggc
    21181 atagcggatc gagatccgcc gccacggctg caccgcatcc ggatcctgcg gatcggtatc
    21241 cgcctcgatc cggacggggc tttcattgtc cttgtcgagc aggtactgca ccggatcaag
    21301 gtaatgctgg cccatgtcgc ccaggccgcc gccgtcataa tcccaataac cgcggaaggt
    21361 gccatgcaca cggtgggggt gatagggctt cttcggcgcc ggccccagcc aaaaatcata
    21421 atccagctcc ggcggcacgg gttggggcgt caaatccgtg cgcccgcacc actcgttctg
    21481 cttccaatca aaacccgtcg ggccgcccag cgtcaccttg agcggccagc cgaacaaccc
    21541 gttctgcacg gcctttttga tcaggcgcac cggcacgccc acgccataaa aagcgccctc
    21601 gaaccggaac caggtattaa gccggaaaat gcgccggtac ttcgccatgg ccgctttgac
    21661 cgcctccccc tcgccaatcg tgcgcgacag cggcttctcg caccagatat ccttgcccgc
    21721 cttggccgcc gcgatcgaaa tcagcgcgtg ccagtgcggc ggcgtgggga tatgcacaat
    21781 atcaatatcc ttccgcgcca gcacttcgcg gaaatcccgg tatcctttca cctccggccc
    21841 gcacttgctc agagcggctt gcaggtgctt cgcgtccaca tcgcagacgg ccagcagctt
    21901 ggcggtgggg ttaatcgagc tcaagtgggc ccgccccatc gagcccaccc cgatgacggc
    21961 tttggtcaat atctcgctcg gcggggtcac tcccggcccg cccaaaacat ggcgcggcac
    22021 caggctgatg gcggcggacg ccaccaggga ggttcgcagg aaatcccgac gggtaaactg
    22081 gcacggttta ttcatacgca atccaaaagc taacagacgg cacctcgcta gaaaatcact
    22141 ttctaaacga aataaaaagg gaaaaagcgc acccttcccc ggacttcacg cccccgcccg
    22201 cccgcaacgc cgccgaaaac cgccaaaaca acctcctccc gccgcccgcc ccctcccaga
    22261 accccgccgc ttattccgcc cgcgcccggc gccaacctgc ttcgcccccg ccaaccccgc
    22321 gaaagccacc atcccccgcc aaccccctcc atcccccctg ccaacccgcc gtgtacaagg
    22381 ggtgtacaag ggatgctcat cgggtgaaca tcggaaaatt cgatgaacat ccggtgagca
    22441 tcccttgtac accccttgta cacggcgggt tggcagccat taaatagcgt aaaatcaagg
    22501 ggtaagtggg tttgggcggc gggagtgtcc aaaaagtgga cacttttatg cggcgcgggt
    22561 gcggagggga ttgaaggtgg ggtgccggag cgatgggttt ggcgcggctg gggagcgcta
    22621 cttgaggcgg agggttggtt cgtggcggaa gcgcagcagg tccgtgtccg tggcggcgtc
    22681 cgcctcatcg ggaaacagga cgacaatcga ggtgaaccgg tctggctgcc attgcttttc
    22741 gtgcaggagc cattttttta cgaactcggc gacggaatgg cggcaggcct cgcggacgag
    22801 cgccaggtga ttggtatcgc cggctcgttg ctccagggcg ggcgtcaggc ttcgttgcag
    22861 cgcctcgagt tgttcgtttt tgtcaaaacg cgcccagccg ctttcggccc gccgttccat
    22921 ttcatcggtg tggatggcgg gcggcaggct ggggcggatg gggggagcca ggaccacgca
    22981 aagctgtccg cgcgccgcca ggcgccaggg atcggaaagc cgcagatggt agcgaaagga
    23041 taccggcacg cgaatctcgg cgacggtggt tcccaggtac acccaccccc agcctatccg
    23101 ccgttcgtcg gtgcgtttga aagtctcctc ggcgcggaag acggcgagtt cgagcacgtc
    23161 gccgtgggtg gcggtgattt gggggatgct ctcacggaag gtctgggtga tccggccggt
    23221 ttggaagttt cgcgcgacct cggcggcagt gcggcctgct tggtccaccg tttcccgcac
    23281 gctccgcatg gtcttgagat aggcgaacaa cgccagggcc gcaaggatga ccaccgtggc
    23341 ggccaggatg gttgtggtcc agggaaagga tttgagaatc gggttgctgt ccatacgtca
    23401 tgcggcggcg cctgccctaa atagacggcg gcggcgtcat ctgtcaaccc tgcgcttgag
    23461 ggatgaggct cttccgggag gtcgggtttg aaggagcacg gacttatccg gccgatcccg
    23521 tccggggttt gcgtgcccga gccggctgcg gagcgaagtt ggggggcaaa aatatccacg
    23581 cctccgccgg tcaaagattc agcgaggggc gtgagcctca cgcaagatgc gtgaaaatct
    23641 cgcgcatttt gccaggtcgg gttgcggatt tcttaggttt ttgaagaaaa taggggctgg
    23701 caccggggtt gctaaataat tggcgaaacc gggttgacag cgtggacaat aagagtaaag
    23761 ttcgcggtct gtttgccaga gaaccaataa aataatatag caaagtttat gaagaaccta
    23821 cgcaaaaaga tcggggcttt cactcttatc gagttgttag tcgtaattgc catcatcgcc
    23881 attctggcgg ctctcttgct gcccgcgctt gcgcgcgcga aatcgcgggc gctccgcgtc
    23941 agttgcacca acaacctcaa gcaggttggc ctggccatgc gcaccttcgc gattgataac
    24001 gacggcgcca tgccgatggg gctcgccggc gcgatgggcg gcgcttcgga agacgtggaa
    24061 accttcgccc gcgtccttgg caccacgcag cttggcagcc acggcgccgc caagatattc
    24121 ctggcttgct cgaacgagtt gagcacgccg aaagtgctct tctgcccggc ggagtatgag
    24181 acctcggttc ggcaggcggc caccagcttt gcggattcga cgggcgtcag ctcgaccact
    24241 gtcagccaga ttccctacac caacgacctg aatatcagct attttgtggg tgtggacgcg
    24301 acggaaaccc agccgcaaat gttcctgacc ggcgaccata acctgggcgg gaatgccaat
    24361 ccgccgaccg tggcgtacct ggcagctccg gcgacgggga ctccgaagat ttccctcggc
    24421 accaatttca ccgcgaatat ggggccggcc tggatggaca atatgcattc caagcagggc
    24481 aacgtcggga tggcggacgg cagcgtgcag ggattcaacc gcgcgcgtct gcaggaagcg
    24541 ttgaagaatt cgagcgacac cggcggccgg acttcgacca agttcccgct ggcgaatggc
    24601 gcgtctgcag gattgaactg caaccgcatc atgctgccgt aattccaacc tggtaattta
    24661 gctccctgga gcaaagcgaa aaggcatccg caaggatgcc ttttctgctt tgtaccgatc
    24721 cccggccgga tattttttgg ctggaggggg tcagaactct ttagaaatgt ccccttaaga
    24781 actcggagaa aatgtcccgt ggtgtggttg tttccgcgtc agggggcggc gggggcgggc
    24841 gcagaccgtg ttcctcggag aagcaaagac gccagcgcgc aaccgcccgc caaaacggtc
    24901 agagacaaag accatctggt gcacgccacc agcggcaggt ttgtgctgcc ttgcgggtca
    24961 gcggttaaat ggatcgtgca gaccgttcca gttgccgttg tcccgtacgc catcgccgtt
    25021 ggagtcgtca attttctgga tccccatgcc ctcggcgtgg gtatcaacaa agacgaaccc
    25081 aggtttgccg ttgttgcgcg gggcgggata ggcgcgcacc tgccagtcgt acggaccggg
    25141 gctgggggtt tcgatcatgc cgtcgtcgcc ttcgtaatat ccggtaagtt ttgcgcccag
    25201 attcaaggaa ggcagcggcg gatcgagcgc gtaaggcgcc tcgggcttgc cggtataaac
    25261 accgcggtgc gatttcgagc cggcggtatc ccccacgaac accgtactgc tcggatgacg
    25321 gatgtcggcg cccagccggg cgctatatcc gggcttccca tgctcgccgg tggcataccc
    25381 caggtagtgc aggttgtaac cgtaaccgcc gtaaatgtac tgccgatacc cgtcaatgcc
    25441 gaacggccgg gaaaaatcct cgctggcaat ttgctcggta gtcagaaatg gactcaggaa
    25501 caccttgggc tggggcgcgt agcagaccat ttggtcgggc cagtgagttt tgaccgcttt
    25561 agtcaatccc aagttctttc cctcctggcg atcaatgatt gccccctggt agtaatagcc
    25621 ttcgtgatgg gcaaaaaacc cgttgctgtc gtcgaggtac atctggttgg cgatgccgat
    25681 ttgccgcaga ttgttgaggt tcgctatgcc cttggcggtg ttttttgcac ggcccagcgt
    25741 gggcagcagc aacgccgcca ggatggcgat aatggcgatg accaccagca actcgatcaa
    25801 agtaaagccc cgccgccgcc agcggcgaca gttgatttgc gcaacggtta catcaaggga
    25861 cttcatgcag atctcctatt cagaggtttt ccaacggttt caggggatag cggcaaggtg
    25921 gggaggcggg attagccggc gaccagatag agcatgatgc acgaatattc gaagccacgg
    25981 aagtgcgcgc tcggcgtgaa agctgtcgcc gcaaccacgc gggtgaggat ggaagtccgt
    26041 ttcataagtt tttcatcagt agctcatggg tgcgcggcgg aagcgtcgtt ggtatcggct
    26101 gggccccccg gcgtccaagt ggcgtctgtg ggcttggatt ttggttctcg gccgcgtggt
    26161 caaccgttct tcccttcgac ccaagggcag gctgcctcac cccattccac ctgtcaaccg
    26221 cattctcgac gcctgttgtg gttaggccgg gagatcgtga acactttttt aggcttctca
    26281 aagggggtca aaactcttta gcaatgtccc cttaagagct caggagaaaa tgtcccgtgg
    26341 tgttgttgtt tccgcgtcgg gtccaataag ggacattttc aaagagtcga gatatttttg
    26401 gctggagggt tgacgccggg aggcggcaaa ggcaaactgc ccgcctatgc tagcttcctg
    26461 tgtagtgcga tggtggttgg ccctgacggt ggcattttca gccgtcacgg tcctggctgg
    26521 gccaaatgaa ctttatgatc ccccggcctt gtttctgacc tggaaacgcg atccggcccg
    26581 gaccatgatc atccactggc aaacggtggg cgaagcccgg gccgaagcct attaccgcgc
    26641 cagcggagcc ggggactgga tcaaggtgca aggggagagc catccttttc ccaagtccaa
    26701 gcgcacgatt cacaccgtgg aactcacggg cctgacgccc cggtcggatt acgagttttg
    26761 ctttttgccc ggggaaaaga ccttccggtt ccgcacgctg ccggccacgc tggtttcccc
    26821 ggttcgcttt gtcaacgggg gcgacgctta ccacgagcgc aactggatgg atacgatgaa
    26881 ccggctggcg ggcaagttcg atccagcctt tgtggttatt ggcggagacc tggcctactc
    26941 ctgcggtggc aagtccccgg agcggatgga gcgatgggat gattattttg attcctggaa
    27001 gacgaatgcg cgcgcttcgg acgggcggct ggttcccctg ctggtcacca ttgggaacca
    27061 cgaggtggtc gggtcctggg accaaagccc caccaacgcg caaggctttt acgccctttt
    27121 tcccttgccg ggagaacgcg ggtatgcgtg cctggatttt ggcaattacc tgagcctgtt
    27181 cctgctggac tcgagcatca cccatcccat cgaaggcgcc cagaccgact ggctgcggca
    27241 tgagctggcc aagcgccgca cgatgccgca tattattccc gtttaccata tcccggccta
    27301 tccttccatc cggtccgatg ccaccggcca gagctccgaa cacacccaga tgatccgccg
    27361 ctcctggtgc ccgctgttcg aagaggcggg cgtcaccgtg gctttcgagc atcatgatca
    27421 ttccaacaag cgcacctacc cgattcggga tgggaagatt gaccccaccg gcatcgtgta
    27481 tctcggcgac ggcgcctggg gggtacacct ccgccaaccc gacaagacca agccccggtg
    27541 gtacatcgcg cgctcggatg ggattcggca tctttacgtg gtgacgcttt acccggaaag
    27601 ctgccatgtt ctggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctgac
    27721 ccggccggat ttggcgccgc cggggagggc gggcgcggcg gggcgcggcc gggagcgcgt
    27781 cagtcggacg cggggcagat ccgcccgagg tgcgcctgct ccacccagcc cagggtgcgg
    27841 ctggcgcgga tgagcacgaa gcggccgcgg gtgcgcaggc agcgggcggg ttccccggcg
    27901 gccaggcgcg tcaccgtttg agcctcgtgc gtgggggtga gccggagcgg ggtattgggc
    27961 ggcaggatga agcccagccg ggagcgggtt tggacgccga tatacgccgg gacacagagc
    28021 agaaagacca tgaaaccgaa ggcggcgacg gcctggtgcc aggtggcttt gcgccgccgg
    28081 aacacgccgg gcagcatcgc tgcgctgatg gccatccaaa agctgccgcc ggccagccag
    28141 gcccaccatg ccaccggcag ccaggtggaa atgacctcgt accacgccag ttcgggcgct
    28201 tcgagctggg ccgccttgcg cgcgtagcgc aggttctggc gcgccgccgg gtcgaacgga
    28261 tgcagccatt gcgcctgttc ccaggccagg atggccgcgc cggtctggcg gcgctgccat
    28321 tcggcgttgc ccaggttcag cagcgtcccg gcggcgggct gccgggcggc ggattggcgg
    28381 aaggcctccg ccgcgcgcgc atagtcagcc gcgcggtagg cgctgacgcc cgcctggaaa
    28441 agggcttccg gcgatggggc ggcccggagc ggccatcccg ccagcagccc ggcaagcagc
    28501 aggacgaacc gcgcgcggcg tgggaaggcg gggcggcgcg tcatagcttt tcctcaagcg
    28561 cggcgagcac ctgctccagt tccggctgca acgaaagcaa ttccgccacg ctcgcgggat
    28621 tggggctgaa gcgggcggca tcggtggcgg caaagaagcg gcgcaccacc tcgcccgccc
    28681 ggtccgaagg ccccgtggcg ggcagcagcg gcagcacgtc gccccccacc agcgcccgcg
    28741 gctccgccgg gtaatgcggc gcgcacgcgg tccgcagggc gttgaccgcc gccgccgcaa
    28801 accggggtgc atccgccgcc cgcgccgccc gccgcaggag gcgccgctgg cgtcgcagcg
    28861 cccgccgggc gcggcggcgg cgcacgacgt ccgggtgggc ttcctgatag cggcggcggc
    28921 ggtcccagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gatgaggata
    29041 tagctgaagg tcagcacgcc gtgaagctcc ggggctggcg gcggcgcgcc ggcggggggc
    29101 ggcgcggacg gcggcggcgg gccgatgggt tggacggggg cgaagtcggc ggtggcgaac
    29161 acctgccagt cccgcgtttg gggcggcggc ggcggggtca cccgggccag cggagcgtcg
    29221 ccgtaattcg tcagggtcac cgtgaggtta acgggttccc cgacgcgcag cgcgttggtg
    29281 gccagttgcg gcgggccgat gcggaagtgg ccgatggcgc cggtgaaccc gggcaactcc
    29341 ccttcgcggg ggaggggccg gacgttcagc tccagggggt cggattcgat cagcgtgtat
    29401 tggacgatgg gcgggggaat gacggtggcg ccggggctgg cggcgggcgg agagagccgg
    29461 ctgctggcca tgaacccttg cgcctggacc gtcaagcggc ccgcggtcag cgggatgaag
    29521 gtggtttcgt aaatgaacga aagcacgctg cccccgttct gcgtcaccgc ttcgacgcgc
    29581 tggcgggacg tggttccgtc cacgaggaat ccggggccgg tcaactgcgg ctgcgccagg
    29641 ccgtggatgg cgtttccggg gcgtccggcc agcaccatgc gcgccatgac cgcctgacca
    29701 acgaacaggt tggtgcgggg caactccagc gtgagcggca gccggggcgg cacggacgcg
    29761 ggcaccgttg ccgccacttc caggcgcgcc gacggcacgg tgacgggttg gccgttaatc
    29821 cgaaccacga actccggcac gacgaacgtg cccgggcgcg ccgcctgcgc gcgcgtatta
    29881 aaggcggtgt acggctcgaa gttgacgccg ctcaccttga gcatctggcc atgggcgccg
    29941 gggcgcagtt ccagccccgg cggcccggca atcgtggcgg gccactcgac cgagtcctcc
    30001 agcgcgttga acgtcacgcg caggaacgac ggttcccccg ggcggaccac cggcgggtcg
    30061 aattcggcgg tggccgtggt cagcgccgga atctcgagcc ggggctgcga gagcatgagc
    30121 gacatcaggg ggtcgcgcga cggggcggca ttggtttggg cggccagcgg ggcggccgcg
    30181 gcgatcaacc cgccggccag cagggagagc attttccggc cccgttcacc cccgcctccg
    30241 gggagccgaa cctggcgctt gagtttcatg tccggagaga aaagcagacg cgcggtcatg
    30301 gcaggccccg gttccggtca ccacgcgggc cgcgagcggt ctcgcggctc gctggtggat
    30361 tcctgcccca tcggcagacg acgttcggtg tcgagtttgt aagcttcgag gagccagccc
    30421 gcctgctccg gggacaggtg catttcctcg ccctcccggg aggggccttc ctgttggccg
    30481 ggctgcggcc ccatcgtctg ttcttcgtcg tcctcctctt cgccggcgac gccgggcggc
    30541 atgtcggcgg cggggatgcg ccccttgagc tttttcatgg cggcgctcag ttgcgactct
    30601 tttccgcaca ggccgctgag gcattgttgc agttcgttca ggctgtccac cagccgggcg
    30661 atgtggcggt cggtcacctc ggcattatgg cgggcgtcgg cggcggcggg gttgagttcg
    30721 aaggcgctct tgaaatcgcc cgcggcacgt tcccagcgct gcagaaccgc gccgtacact
    30781 ttgagcgcct gccgcacggc cgcggttgcg gccttcaatt cccggcgcgc gccccagccg
    30841 cgcaggtagg cggcgaccat gcgctgcacg tcgtcgtccg ccagggccgc attcgcctgg
    30901 cggagggcca ggtccgtctc cccggccgcc gcctggccgc gcgcgagggt gggctggggc
    30961 gccgggcctt tcttcccctg ctcgacgccc tgggcgaacc gggtgaggcc caggttgtaa
    31021 agggcgtgcg gctggaggga gtctttctgg cgggtgagcg ccatttccag aaaggcctcc
    31081 gcctcgcgga gtttgccgct gtggagctgc cgcgtgccgg cgttgaagaa ctcgcgcggg
    31141 gaggcgggcg ggggcggctc cggggtggct ggcgcttcgg gttcctgaga tgggagtggc
    31201 ggcggggccg ggggtgagat ggcttcggga gcgtccccgg cggccagcgc cccggcgaga
    31261 agcaccgggg cggcaaatcc ccaaagtgat attaaaaggc gcgccatcac agttcaggca
    31321 ttaatgtcgc gcagacggcg ccgcgtgcca atcagagatt cgaggatgag cagggccaac
    31381 acgcccgcca ccggccaatg gaagcggtcc acccccagct tgcgcgccgg ggctgaagcc
    31441 ggcccggtct ggaggtccgc cagcgccagt tggacccggg ccagcccctc gcccagcggg
    31501 ccgagcggaa aataagcgcc gccggtggcc tgggcgatgg cgcgcagggt ggcttcatcg
    31561 agccggctgc gcaccacctc gccgtggcgg tcgcgcagcg tctcccgttg gccgtgctcg
    31621 ttggtgaatt gtatctccgc gccggcggcg gtgccgacgc cgatcgtaaa gacgattaac
    31681 ccctgtttgg ccagggtttg ggcggcgcgg atgccgccgc gttcgaggtc ttcgccgtcg
    31741 gtcagcagca cgcacagccg gggcggccgg gacttggcca gcgcgcggga ggcttcgtcc
    31801 agcgcgcggc ccaggtcggt gccgggaacg gggatggcct ggtcgtccac cgccgccagc
    31861 gcatcctgga aggccgcgta atcatacgtc agcgggcatt ggaggaaagc ctggccggcg
    31921 caggccacca gcccgacgcg gccccgcccg cggtgctgca cgtagtcccg gagggcgagt
    31981 ttggtgcgct gcaggcggct gggggccagg tcgctcgcca gcatgctgcg cgagcagtcc
    32041 aggataaaca gcgtgtcctg gccgtagaga tggctgattt ccgtttgctc gccccactgg
    32101 ggccgggcca gggcaagccc gacccccgcc acggccagca gcagcaggac gtttttgagc
    32161 aggtagcggg cggggctgtg ggagcgggtt aacgcggcga ggaactccgg cgcggccatc
    32221 cgcgcgagtt gccggcggcg cgccctggcg gagtaccctt gcagggccgc cagcagcagc
    32281 ggggcggcca gggccagcca aagccattgc ggttcggcga aatggggatg gctgaagtcc
    32341 atcacggcac cctccgcagg cgggtgtggg cgagcgtcag ttccagggcc agcaggccga
    32401 acgcggccag cagcggccac tggtagagcg gccggtaggt ggtgaagcgg cgcagcttga
    32461 tttcggtttt ctccaggcga tcaatttcgt cgtagatgcg ccgcagttcg cggcggttgg
    32521 tcgcgcggta gaacttggcg ccggagagcg ccgccatttt gcccagcacc gcgtagttgg
    32581 cgggttgcag ggtcatgccc ggccggagat tgccgtgggc atccagctcc agtttgccgg
    32641 tggacgggtc gaagagcggc agcaggcacg gctgctcgat cccgatgcca atcgtataaa
    32701 ccttcactcc cagcgccgcg gccagttggg cggcgggcac cgggtcaatc tcgcccttgt
    32761 tgttatctcc gtcagtcaac aggatgatga tgcggctttt ggcgtccttg aggttcttaa
    32821 gtcgcttgac agcggcggcg gcggcgtcgc caatggcggt gcccaggtcg cgaatcagcc
    32881 ccacgtgcag gcggcggaga ttctcgatca gccagtcgtg gttgagggtg agggggctgg
    32941 ccaggtaggg cactgcggaa aagacaatca gccccagccg gtcgttggtg cgtttgcgga
    33001 tgaagtcctc gatgacctcg gcggcaatgt cgaaccggga aatccgctcg ctcggcgcgc
    33061 ccatatccag cgccatcatg gaccacgaca aatcgagcac cagcatgatg tccaccccgc
    33121 tggcctgcat ttcggtgtgg tcgttggcaa agcgcggccc ggccagcgcc acgatgcaca
    33181 acgcggccgc caggaggcgc aggaacagca gcagccgccc ggcggcggag cgcgcggcgg
    33241 ccccggcggc gcgggcgatg tcggcgctgg ggaaacgcag ggcggcgagc cggccggcgc
    33301 ggccgcgcag aaaggaatag atgggcagca gggccagcag ccccagcagc cacggatatt
    33361 gaaactccag gttgccagtc atgggctcaa gccgcgggtt ggcccgcggc ggccggggtg
    33421 gcgccgtccg gatgcggggg cgcgcgccgg gcttccgcct gctcgaccag ttgcaacgcc
    33481 cgggaaacgg cgtcgagcgg gggtgcgggg gcgggcgggg agaacttgta ttggtcgcac
    33541 gcgcggagga actccgcgag cgcggtcgaa agctcggggc caatggccgc gtggccggcc
    33601 acggcgcggc aaaactccgc cgtcgtcatt tcctccggcg gcaaagcaaa ggccgcctgg
    33661 acataatgcc gcagcacttg cgagacgcgg ctcagcagcg cgccgtcttc cggttgatgg
    33721 cagaggggct ccagcgcctg gcgggcttgc gcctccgggg gcacggcttg cggggggcgg
    33781 ggccgggtca ggaaccagat tgccgcgccg gccagcgcca ggaggaggaa tccggcgatg
    33841 atgacccaga ggccggattg ctcccagaag gtgggcggca gttccgcgcg cggcgggcgc
    33901 aggggcggga tgtcgtcgag ttcgggaatg cccgccgccg cgcgcgcgag aaacgcggct
    33961 ggcagcagga ggcggaaaga accagcgtgg agatcgggct tcccgccgcg gcgggcatgt
    34021 tgccttctgc cggctggcgt tctcacccgc gtctccttcc gcggcgggtt tcaaagaagg
    34081 tttgcagagc ctgcgcaaag ggttccgccg tgctgaagcg cagggtgtcc acccccgcgc
    34141 ggcgcagggc gcggtccagt tccgcgaggc gcgcggcatt cgtgtgggcg aatcgctcgc
    34201 ggacggcggg gcggctggag tccagttcca gcagttcgcc ggtctcggcg tcctcgaggg
    34261 tcagcaagcc cgcgggcggg agctggcttt cgcgcgggtc gtgcagatgc acgcaaacca
    34321 ggtcgtggcg ggcgttggtg aggccgattt cctgcacggc atcccggccg gcgccgggcg
    34381 gggcggcggg gggcgcgaag ctgtggagga aatcggtcag caggaaaaca atcgagcggc
    34441 ggggcaggac gcggttcagg aaggcgagcg cgccggggat gctggtgccg cgctggcggg
    34501 gtttaaagag cagcatctcg cggatgaggc gcagcaggtg gcgccgtcct ttgcgcggag
    34561 gcaggtagag ttccacccgg tcggagaaga gcagcagcgc caccttgtcg ctgctgcgcg
    34621 cggcggagat ggccagcgtg gcggcaatct cggcggcgaa ctggcgtttg gtgcggtgct
    34681 gcgagccgaa cgtcgaggag gcggaaatgt ccaccgccag caccatggcc agctcgcgct
    34741 cctcgcggta gcgcttcacg aagggacgcc ccatccggta ggtgacgttc cagtcaatgt
    34801 cgcggacttc gtcgccgggg atgtattcgc gcagctcctc gaagtccatg ccccggcctt
    34861 tgaagtggct gaggtaggcg cccaccatgg tatcgttcac gaggcggttg gtgcgcacct
    34921 cgacgcggcg gacgttttcg agcagctccg agagggtcac ggcaccggca ccgcgttgaa
    34981 gattttccgg atgatggcgt cggtcgtcac ggcctgggcc tcggcttcgt aggagaggag
    35041 gatccggtgc cgcagcacat cggggccgat gctcttgacg tcctccggag tcacgtaggc
    35101 gcggccttgc agcagcgccc aggcccgggc ggccagcgtc aggtagatgg tcgcgcgcgg
    35161 cgacgcgccg aactggatga aatgcttcag gtccagttgg taggcgtccg gcttgcgggt
    35221 cgcaaacacc aggtgcacca cgtaatcgcg gaccttttcg tccacgtgaa tctggtccac
    35281 cagcttgcgg gtctggagga tttccgccag cggaatgacg ggctggactt gcgtttcggg
    35341 cgcggtgaag gccatgcggt caagaatctt ccgctcctcg gcgaacgatg ggtaatccag
    35401 cagcaccttg aacatgaagc ggtccacctg cgcttccggc agcgggtagg tgccttcctg
    35461 gtcaatcggg ttttcggtgg ccaggaccag aaacggcgcg ggcagcggca tcgtttcccc
    35521 gcccagggtc acctgccgtt cctgcatggt ctccagcaag gccgattgca ccttggcggg
    35581 ggcgcggttg atttcatcgg ccagcacgaa gttggcgaac accggcccct tggtggcccg
    35641 gtactggccg tcctggggat tataaatcag ggtgccgatg atgtcggcgg gcagcagatc
    35701 aggggtgaac tggatgcgat ggaacgtggc ctggatggcg gaggccagcg tgcggacgca
    35761 cagggttttg gccaggccgg gcacgccttc gagcaggacg tggccgttgg ctagcaggcc
    35821 gaccagcaac cggtccacca gttgctcctg accgacaatg acgcgcgcga cctccgcgcg
    35881 cagccggcca atccagccgg tcgcggcctg cacctggccg ttcaaaggtg tgttcgtcat
    35941 gctccgactc taggcgagtg cgcgggccga ctcaagttat ctgcgccggg agcggctttt
    36001 ttggcctcct cggttccggc cggtggcagc ggggagattc cgccagccgc tccccgggac
    36061 ggggtccggc ccggatcagc ttttcggccg gagcaggatg gccttcttgt cggagccctc
    36121 gacctcgacg ctttgggttt cgacgcgcgg gtcgtccttg agcgcctggt ggacgatgcg
    36181 gcgatcgaac gcgttgagcg gttccagttc caccacgtcg ccccagcggc ggactttttc
    36241 ggcggcggcc tgcgcctttt tgaccagcgc ctcgcgggcc tgggcgcggt agccgctgat
    36301 gtccaccatc actttgggga ccgagggatc ttgctggaag agcaggcggt tggtgatgta
    36361 ttggaggtcg gccaggcact gaccctgccg cccgatgagg cggccggagt cttcggtctt
    36421 cacatccagc aacagcccct cctccaggtg gtgctcctcg acggtggcgt caaagccgag
    36481 gagatcgagc agcttttgca gggtggcttg aggttcagca ggcataacag gatgagtcgt
    36541 tgagcgttgc ttgttggtcg ccgcaaaatg ccgggtccat caggcgttat ttcttctttt
    36601 tcggcgacgg tgtcaaggcc ggagctttgg ccggaaccgg ggccgctgcg gcgggctccg
    36661 ggagggtgcg cgtcagcttg gtctggagga tggtcaggag gttttgcacc gtccaataga
    36721 gcgtcagccc ggcggagaaa ttatagagaa acaccaggaa catcaacggc atgtagcgca
    36781 tcattttggc ctgcatgggg tccattccgg gtgagggcgg ggtgaggcgc gcctgccaga
    36841 gcatggtcgc gcccatgatc agcggcagca gattgaaggg gaaatgcgtg cccggaatga
    36901 cgaacagcgt atcgggttgc gacaagtcct tgatccagag aaaacgcgcg ccgcgcagct
    36961 cgatggcgct ctggatcatc cggaaaaagc caaagaagac gggaatctgc agaagcatgg
    37021 gcaggcagcc gcccaggggg ctgaccttgt gctccttcat gaactccatg gtcttcttgt
    37081 tcattttggc cgggtcatcc ttatactttt cccgaatggc ggtcatctgc ggctgcagcg
    37141 cctgcatccg cttcatggag cgcgtgctgg cctgggtcag cggccagaag aggagcttga
    37201 tgatgatggt gatggcaata atggcccagc cataggagag tcccagcgcg ctatgcaggc
    37261 cgttcatggc cagcagcagt cccttggcaa agaagccaaa gaagccgctg tagcccatca
    37321 ccaggtccag gttgttgccg aagcgggcgg caatgcgggc gagcgtccgg tattccttgg
    37381 ggccggcgaa caggatgatc tgattggtca ccacctggcc ggcgtcgagg gtttggcccg
    37441 ggtaaaccag cgccccttcg tagccttcgg acggcgggct ggcgacagcg ccgaaccgtc
    37501 cttcttcggc gggcggcggc ggcaggagga ccttgcggac gacgatctgg cccgctggtt
    37561 gctggggcat cacggccagc gcaaagaact ggttgtgcgc ggcggcccaa accacattgt
    37621 ccgcgcccgc gcgatactcg gcgggcggga cgcgcggcat gcaggcgaat ccgcgcgtgg
    37681 aaaagtagct ggcgccggcc tggtcaaacg tcttggcgcc gttataccag attaccccca
    37741 ggctcaggcc cttgtcatcc gcccccaaag gggtggccgt gccgacgacc cattcctgcg
    37801 gcggcagggt cagcggctgc ggcgagcggt tctccagccg gacggtcgcc gtcaggagat
    37861 aattcgtgct gagctgaaac tccttcacca ggcgcaggcc gttggtgagc gtcttttcgg
    37921 cgcgcacccc gtcgccggtc tgcgtcagcg taaaaacccc gtcgctggac agggcgtcgg
    37981 cgcccaggat cgcgagcgcg ggggtggggg tgaaggcatt tagtgtggcc aggttattcg
    38041 tgggcgccgg cttcgtgcgc cgggaggaca cggtttccgg gtaatggatc aactcgacct
    38101 gtttcaagcc tccgccgtgg gaggtgaacg tgtagcgcgc gtcgttgtta gtaaggatca
    38161 gcaaccgctc cggttcggtg gcggccgccc aggcccgggg agcgggcgcg ggggcctcgg
    38221 agatgacggg gggcgcggaa ggagcggctt cggccgggtt ggtgggggcg agcgggctct
    38281 ggacggcgtt ggtggcgccc ggagggagcg gtttgggcgg gtaaagcttg ttgaccagcg
    38341 gatgccacag catcagcaga atgaagcaaa cgaccaggat gaggattgac ttgcgatcca
    38401 tgcgggcggg cgcgcttacg aatgggcgga ttgccgctcg ggatgaaacg gaaggcccgg
    38461 ggaaccctgc cggttttccg cccgggaggt ttcggcggag ttccggtggc gggccgcggg
    38521 ttttccttcg gggacggggt caaagccgca gccgccccag ggatggcagc gggcgatgcg
    38581 cttcatcgcc agccagcttc ctgccccggc gccgtggtgg cgcaaggctt ccagggcata
    38641 ctccgagcag gtgggcgtaa accggcagcg tcccagcggc ccgaatagaa acgtcttggc
    38701 cggggatagc gtccagcgat agaggcgcac gccaaaaatc aggatgcgct gcgccaggtt
    38761 catgcgttcg ggcgccgggc gggagacagc agcccggctt tccgcaaagc ggccaggtaa
    38821 tcccgctcca cctcggcaaa ccccttgccg gtgatggatg cccgcgcgac cagcaccagg
    38881 tccaccggcg tggccaggtc gtgctgatga agccgaaacg cctcgcgcaa cagccgccgc
    38941 gcccgattgc gcaccaccgc gccgccaatc cggcttccgg tgatgacgcc caggcgcgaa
    39001 gccgcctccg cgggccgccg ctgccagttg gcaatcaggc acccgctcac ctgccgctct
    39061 ccggcctggc gcaggcggct gaaatccgcg ccgcgcttca gccgcgccgc gcggccaaac
    39121 cgcaagcgtt gtggaggggg gatggtcatg ggccccggag gccgttatac cggggtcagg
    39181 cgtttgcgtc ccacgcgccg gcgattccgc agaatgccgc gcccgctctt gctcgcattc
    39241 cgggccagga acccgtgctg ccgccggcgc cgaacctttg atggctgata ttgtcttttc
    39301 ataattcagt tgcgttcgtt ggccgcgagt tattcgcctg agtgccgccg cgtcaggaaa
    39361 cactccgtca acgaagcacg ctagactagc agcaacccgg cggaggtcaa gcaagggaat
    39421 tggcggaaat gtcctcgccc ggctgccccc cgcgccgtgg ccgcggccag gggcggaata
    39481 agtgtcgttc gcgctgaggc gatcgggtaa aaccctcggc gtgaagcccg accggctccc
    39541 atggcggttg cgtggtcgcg aaacggacgg cgaggcggcg cgcagcggcg gcgcccggcg
    39601 cggccagtct gccgcgcgaa atttctccgc aaccggctga ccatgaaaac ctgggggcgc
    39661 ttgctttgct ggaccgcgat cgccgccgcc gcttttcacg gggcttatgc ctccgcccac
    39721 accagcatcc tggtcgtgct ttatctttac gcgctgctgc gactggcccg ggccgaccgg
    39781 tggcgccaag ccttctattc cgggctggcg gccggatttc tgatcgccgt ggcgcggctg
    39841 gcgttcttct ggcggctctt ttccgccggg gcgatagggc tctggctgat ttacgctttc
    39901 tgggtggggc tgtttgtggc cctggcgcgg ctgtgtctca ggcgatttgg cgcgccgggg
    39961 ggctggctgc tgattccgtt tgtctggacg gggctcgaat acttccgcag cgaactttac
    40021 tacctgcgtt tttcctggct gaacatcggc tacgccttcg cgggcgcgcc gtggcaggcg
    40081 ggattgaagc tgacgggtgt atatggcgcc gggttcctgc tggccggcct ggccgccgcc
    40141 gccgccgggg cgcggcggaa atcccggctc cgggccgggg cggtgctcgt gctcggcgcg
    40201 ggcgcggttt gcctgggagg ctggctggcc gggaaagtgt ccccggcccc gtccgtggcg
    40261 ggcatccggg tggcgggagt gcagatggaa ttccccacgg aagcggaggt ggttttgcgg
    40321 ctggaagacc tcgtccagga acatccggaa gcggagttgc tggtgctcag cgaatacacc
    40381 ttcgacggcc aggtgcccgc ggcggtccgg gcctggtgcc gggaaaaccg gcgctacctg
    40441 gttgtgggcg gcaaggaccc ggcgcccggc ggcaattacg acaacaccgc gtttgtcgtg
    40501 gggccggacg gcgacatctg ctttcgccag gtcaaggccg tgcccatcca gttcatcaag
    40561 gatggccgac cagcccggga gcagaagccc tgggcctcgc cttggggaaa gctcggcctt
    40621 tgcatctgtt acgacctgag ctacacccgc gtgacggacc gcctgatccg gcagggagcc
    40681 caggccctga tcgtgccgac catggacacg gcggactggg gtcgcgccca gcacgtcctg
    40741 catgcccgcg ttacgccggt tcgcgccctc gagtccggcg tgccgatctt tcgccttgcc
    40801 agctcgggca tctcccagtt gaccgatggc gccggccgtg ttctggccac cgcgcctttt
    40861 cccggcggcg gagccacgct ggcgggaaca ttggaattgc gcgcagccgg ccgtttgccg
    40921 ctggaccgcg ggctggcacc ctgcgctgtt gcggtgaccg gggcgttgct gattgggttt
    40981 gtatttctgc ggcgtcccag tgtccgccag cctccggctc aggagaactg cggaatggtt
    41041 gcctgacccg ccccacaaga aaagacaccc aaccgacgcg cgggcctctg gcccgctttc
    41101 agcgtcctat caacgaagcg tggcggattt gtccctcagc acaggcaatc ctggctattt
    41161 ttgcatccgg gcgggatttg gaggttgtgg ggaagggcaa tccgccggat tataggctgt
    41221 agtcccgctg ctgttagtag aacattcaaa gcgggccgga ggcccgcgcg ccggggcgga
    41281 aatggcatat tttggttaat ttttgacttc cggcgcgaag atttagggta tgttaacgtt
    41341 aatgatactc taaactggag cggtttagct ggaaaatcca acagtttggt gtcatttcac
    41401 cggaatctgg agaggtatgg ccgttcggat cgtagacttt tcaggccttt tggctgacga
    41461 tttccacgat ataattgtcg aatccatatt ggctttcggc tgtaaaatca gcgatttacg
    41521 attgcgagga gagatggaac cgaaggggaa cctaaattac tccggcctag tcgcgccttc
    41581 atccagatgc cttggttttt ccttactact acgccgtgta caaggggtgt acaagggatg
    41641 ctcaacggga gttcataggc ttttagctgg tacacccgtt gagcatccct tgtacacccc
    41701 ttgtacacgg cgtagaggca gggtaaattc aaggtggttc cggggtatgc cgaagtcggc
    41761 atgattctgc ctctatttta tccctcccta tagataaacc gttgatttta ctgacgaaag
    41821 gctcgggatt cagcagccat gcctttgaat ttgctaaata aagtgctcgc cggagctaca
    41881 gtctggacga tcaggattgt ccaagctcca ataaaccgag ggaaagcggt tgaactaccc
    41941 aactga
//
LOCUS       DAOPQG010000015        41801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1233_length_41801_cov_13.766924, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000015 DAOPQG010000000
VERSION     DAOPQG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 41801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41801
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1233_length_41801_cov_13.766924"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1642
                     /gene="wzx"
                     /locus_tag="PKI07_02920"
     CDS             272..1642
                     /gene="wzx"
                     /locus_tag="PKI07_02920"
                     /inference="COORDINATES: protein motif:HMM:NF041503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-unit flippase-like protein"
                     /protein_id="HOC50035.1"
                     /translation="MHSSTVAWFWVFNGLRLATGILLLPLVLRKLSTEELGMYYVFLS
                     QVALAPVIDFGFSPTILRFVSYAMGGAETIQPQGVSKSTGTGPNFGLLWQLFFTTRAL
                     YRVLALVLLVILGIWGTYIVNLRVLETPCPRVTWVAWGITLIATVLNIYSSWAPVFLR
                     GMNEVLISVRITVLASGVRFAVAAGLFLAGAGLMSLPLGDLTGSVIQQVIARGACLRR
                     LRRAPSTKRSDVWQQLRIIWPNSWRLGLVTMGGYLTVNANTLICLKVLGLAANARYGL
                     SIQLLGIISGMASAWTQVKWPIIGQHRARHDYAAIQQIFWPRMWLQYLTFLAMAAGLL
                     VCGQPLLQLLGSNKQMLALPWLALLALNSFLEMQTSIWGTLITTENRIPFLWPSLASK
                     LLSFGLSLTFIYTTSLGLGALVLAPFLAHCLFDYWYWPIHAARGIQTSLARFMFSRPK
                     GKPGTL"
     gene            2243..3181
                     /locus_tag="PKI07_02925"
     CDS             2243..3181
                     /locus_tag="PKI07_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC50036.1"
                     /translation="MPGPICAAVVLNYNGRHLLEENLPSVISAAQRVKEGCDVIVLDN
                     LSTDGSVEFLKKNYPGVIVEIAPSNRILFSYNELCRRIQYQYVALLNNDLRLYEDSLA
                     VLLPHCAGQDVFAVSAVLLDAEGTTITRSRTLGESHRGLFRVAALQESETVPTLVAGC
                     CAVYDREKFVALGGFDELYWPFYWEDNDLSYVAWKRGLRVLIEPRSVMCHRHHATIAS
                     NSQNKAVGHCNAHLFFWKNITDPALIVRHLAWLAFLVPWRAVRGHWPYVRGVWAACRK
                     LPQALKRRREMKPLWVRTDAEVWALVRGDGEAVPPG"
     gene            complement(3281..4081)
                     /locus_tag="PKI07_02930"
     CDS             complement(3281..4081)
                     /locus_tag="PKI07_02930"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOC50037.1"
                     /translation="MKIFVLGHKGMLGHVVARFLKEQGCEVITSSARYEAAARDSLVE
                     AVRNSGCAWIINALGRIKQKTENPGELYLVNSVFPLHLRARMRPEQRLIHASSDCVFS
                     GRRGQYSVADECDAEDVYGLSKALGEAAADSGRCFIIRTSIIGPELDTGWGLMGWFLR
                     QSGEVNGYTNHLWNGITTLEWAEIAWKLINGLIRADEPVIQAGTWPPMSKCQILQLIS
                     KIWEHPIEVRSTMAKEAIDRTLLPTLQRPGLEQQLSSLRSWYELSRPT"
     gene            complement(4078..5067)
                     /locus_tag="PKI07_02935"
     CDS             complement(4078..5067)
                     /locus_tag="PKI07_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOC50038.1"
                     /translation="MKNLSGSVILITGGTGSFGNRVARYLLKQKPAQIRIYSRDEKKQ
                     WEMQRIFPQFRYIVGDVRDPNRLAEAINGVDYVFHAAALKQVPACENYPYEAAKTNII
                     GSQNVCEAAKYNRVKCLVALSTDKAVKPVNAMGISKAMLEKIVCSQNQFDHQTTFCCV
                     RYGNVMGSRGSVIPLFRDQIRQGAPLTLTVPEMTRFLMTLDQSVDLVLHAMTHARGGE
                     IFVRKAPACTVQALAEAMRLKYSPRGARHPIKVVGIRPGEKLHEVLVNEYEMQRMTEQ
                     PSYYAIHPEYRVPQKLTPKPLGQEYTSANTQRLERTEEIQAMLDAMGETEFYL"
     gene            complement(5373..6527)
                     /locus_tag="PKI07_02940"
     CDS             complement(5373..6527)
                     /locus_tag="PKI07_02940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC50039.1"
                     /translation="MKIKVAFLTTDNRDMQRDYGTPVPHFGTAPEALLEGFKQRPELE
                     VHVVSCARVRMTSPQKLAPNIFYHSLFVPKIGWMRTGFQGCIRAVRKKLREIRPDLVH
                     GHGTEHGCALSAVFSGFPNVITIHGNMAELARLFQERVGSYLWLAGRLETLALKRTLG
                     VLCNSAYTEQLVRPRTRRVWRVAHALRQEFFAPAPVPTRHNRCRLVNLGLIAPRKRQL
                     ELLKVAHELAQQGLAFEFLFVGNITPNHPYGNAFQESLKPLEKQGCARWIGTKTSLEL
                     VELFDAASGMVHFSPAESFGLAVAEGLARDLKLFAARVGGVVDIAEGVPGAELFHGED
                     WSGLTRALAHWIQSGFPRAHGAGQVMRTRYHPEVIVRRHLEVYQEVLGQK"
     assembly_gap    6946..7045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7046..9286)
                     /locus_tag="PKI07_02945"
     CDS             complement(<7046..9286)
                     /locus_tag="PKI07_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOC50040.1"
                     /translation="MTPSALPIREIHSQIVHILRTGNRLVLVAPTGSGKTTQVPQMLL
                     EAGLAGGKKIVVLQPRRVAARTVAARVAWERQCPLGTEVGYQIRFDDQTSLGTRISYV
                     TEGILLRWLQDDRTLSNVGAVLFDEFHERNLLSDVALALVKRLQQTDRPELKLTVMSA
                     TLEAEPVAQYLGGETGGAPILLSEGQSFPVAVRYLNRRDERPIPEQAADAVEQIINAG
                     DPGDILVFMPGMAEINATLAALRTLRSPERLALIPLHGDLPPEQQDLAFAPSSLRKVV
                     VATNVAETSVTIDGIRHVVDSGLARIARYDAERGINTLFIEPISRASAEQRKGRAGRT
                     APGTCLRLWTESGQLNRPERNTPEIRRSDLAEVVLLLHSLGIQHAAAFDWLDKPDPSA
                     VERAEQLLLTLGALREPPPQGPAASSGAAGLTPIGRQMLKLPMHPRYSRMLVEAARFD
                     CVPSAALCAALVSGRDLLMRLGRDDKPVQKARELFEASPESDFYTLMRAFQFARNHSF
                     SAESCRRYGIHAQTARLVEQTFAQLLQIARQQGLIKNTATGSQTADNLTPPLSPSSPV
                     SDDALPRCLMTGFIDQLCLRRDQGTLECELTEGRRGTLMRESVVQSAPLFVAAVLREV
                     AGRGTEPRTLLGLATAVKREWIEEVFPEQLAARVEHLYDRAHKRVAAVRLVRFRDLII
                     HHEHQRETDPAASGHCLAEAHQKGWFALPLFNHEAKQFINRVNLVAAALPELEFPSFD
                     DKALRQC"
     gene            complement(9283..9426)
                     /locus_tag="PKI07_02950"
     CDS             complement(9283..9426)
                     /locus_tag="PKI07_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50041.1"
                     /translation="MPPHLVLWISLRLLQTEPAFRSSRTRNGRPRNSRLAIPCCTQTG
                     LTL"
     gene            complement(9514..9589)
                     /locus_tag="PKI07_02955"
     tRNA            complement(9514..9589)
                     /locus_tag="PKI07_02955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9554..9556),aa:Ala,seq:cgc)
     gene            9898..9985
                     /locus_tag="PKI07_02960"
     tRNA            9898..9985
                     /locus_tag="PKI07_02960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9931..9933,aa:Leu,seq:taa)
     gene            complement(10267..11271)
                     /locus_tag="PKI07_02965"
     CDS             complement(10267..11271)
                     /locus_tag="PKI07_02965"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOC50042.1"
                     /translation="MKKNLLAFLYHAMLAAGITGYTLAAEDTLPSLIVAPFSGDTTAI
                     QYWQPALGQGLSEMLITEMGKLNKFQVLESTQLGTLMDEIKLGEDGWVQQDEKVEKGG
                     FAAADFMLTAKVTRFGNKQTKVGLGGFVPGSLGNLGLKQTTADVRIDWRLVDAATRKI
                     IKTGSATAAQKGTGFDVGVNVTGHGGGIGFDNKEFMDSALGKATVTALNQITEDVRAF
                     SLPESGRRKQKANAASQQAAAAQAAAQAARATPGKVLAVAGKDAVIVSLGSNLGFKVG
                     DKLDLYETVDIKDASGAVVFSEEKLIGEVILQTVQADRSKASYSGNAEVKPGWVVKAK
                     "
     gene            11664..12068
                     /locus_tag="PKI07_02970"
     CDS             11664..12068
                     /locus_tag="PKI07_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOC50043.1"
                     /translation="MNIILQDLLELQTLEFGEATGKNIEARVAALRAKLPPPIVGHYD
                     RLRVRGRKGMAMVRNQVCTGCHMRVPIGTITELMRGEDIQLCESCARYLYLPDSAKTE
                     VKKPEETAKPKAKAKVKAPAKPRKRRSVTSAA"
     gene            12226..14316
                     /gene="hyfB"
                     /locus_tag="PKI07_02975"
     CDS             12226..14316
                     /gene="hyfB"
                     /locus_tag="PKI07_02975"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HOC50044.1"
                     /translation="MNGNVIDLPSSFLLQCALWAYVAGGLGSLIAWRRDRLATLIGFG
                     GATLASGCGISAAILTLAAWPASAGKAFALGASGVPSLPLIVQLDPLGAFFVLIVSVL
                     ALALSVYSFGYVRGYYGRRNVGLLGAFYNALLLATTLVFVASNALFFLILWEIMALTA
                     YCLVSFEHERPETRNAGVLFFIMSHLGTGCLILGFLLLFQAAGQVLGNYGPDSYSFES
                     FRALGNSLPPGPRNAAFLLFLVGFGVKAGIVPLHIWLPAAHPVAPSNASALLSGVMIK
                     TGIYGLTRVLFDFMGTPPNWWGVTLLTLGTLSAVLGVLYALMEHDLKRLLAYSSIENI
                     GIILMGLGAALMFLHTQHPVLASLALIAGLYHTLNHAFFKALLFLGAGAVQHALHTRN
                     MEEMGGLIKRMPQTAFCFLLGAVAISALPPLNGFVSEWLTYQSLLQGFGTTGSLVKLV
                     FPLSGAMLALTGALAAACFVKAFGITFLAQPRSEPAAQAREASPAMLSGMGLLAAGCV
                     FLGLFPTALITLFGPLTQQLLGQPLPGYLVAGHGWILGNTQELGGTVSTPGIVLAGIC
                     LLPVPLVLWLFFGRRTRVRIGPTWDCGLRGLTPHMQYSATGFSKPLRVIFKALFRPRR
                     EMQREYDYSPYFATTLRFESRIEEAFVTRLYRPLKRRILLTSRRMRALQAGSIQAYLI
                     YIFVTLLLLLIFAL"
     gene            14313..15254
                     /locus_tag="PKI07_02980"
     CDS             14313..15254
                     /locus_tag="PKI07_02980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOC50045.1"
                     /translation="MKIALAILLQTVLLLGLAPFISGCIKTLKANLQNRRGPRAWQPY
                     LDLWKFLHKDMVISEHASWIFRATPQVLFFTTLLAGLMMPLVSVSAPLSLFGGVLAFA
                     GLLALGRFFLALGGLDPASAFGGMGSSREMTMAAIAEPALMLAIFTVAIEARSTNLSQ
                     MLLSAQAPTWQLLNPAHVLAFAALFIVLLAETGRIPVDNPATHLELTMIHEAMILEYS
                     GRYLALIEWSASIKQLLLMALLLNIFFPVGITADLSVGAVGLALFWFVAKLLALSALV
                     VLVETTNAKLRLFRVPDLLSAAFILGALALLSTFLFQ"
     gene            15251..15922
                     /locus_tag="PKI07_02985"
     CDS             15251..15922
                     /locus_tag="PKI07_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50046.1"
                     /translation="MKPLNPEFASQWITLLATGMLVVQLLMVVQRMLLTNIRLFALQS
                     LMLAGIAVIIAFSHAHAAHVYWVAGLTLVGKVFFLPWLLSRQVRRIQMEHEIAPLLNA
                     SASMLLCGAFTLLGYIVARPFTSLARLGNNTLGVAVTLLLTGFFLMVNRRKALTQVLA
                     LLTVENGVMLAAVALTTYGMPLVVELGIFFDLMVAVMVLGLLVYRIRETFSSMDVSKL
                     SQLKG"
     gene            15919..17400
                     /locus_tag="PKI07_02990"
     CDS             15919..17400
                     /locus_tag="PKI07_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOC50047.1"
                     /translation="MTPLVPILVVPTAAGLLCLLLHSRRAMALANGLAFTVTLGLSVW
                     LLLAMRPGPQVVTECNEFFRADALSAWMALLISVVSLGSSIYAGRYFQRDLAAGAVTP
                     GRVKEFYVLTPLFGAGMFLVVLANNLGVMWLALEATALSSVLLVALYNRSTSLEAAWK
                     YVILGSLGLALALFGTVFTYAAAIDQQSAVELPSFNWSHLMSISGQLNPHMIKLAFVF
                     VLVGYGTKAGLAPMHNWLPDAHSEAPSPTSAMLSGVSLKVALYALVRFHILTTASLGT
                     SFSQTLLLVFGVTSMCVAAPFILVQSNLKRLLAYSSLEHVGLICAGIGLNSRITIFGA
                     LLHMGYHALAKPVLFFAAGNLHQTCHTLQLRRIGPGMARVLPLTAFCLGLAGVAAVGL
                     PPFGLFFSEILVLSGGFAAGHLTATVLLLVAILASFCGLLYQFARILPGEPKVARRSV
                     PAPLAGAPAMLLLLGALLVFSVWLPAPLLEVIRQATDIIGGAP"
     gene            17397..18992
                     /locus_tag="PKI07_02995"
     CDS             17397..18992
                     /locus_tag="PKI07_02995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOC50048.1"
                     /translation="MNALPELQPILASLTERFGAQIQPMQIPRPNEVYFQGKMELVPG
                     FCALLYKEWGARLASLFADDVRAQTGDFHLYYVFALDAAHGFLILRVPVPPSQPRFVS
                     LTNALPALNWQEREIQDLFGLKLEGHPNARPCALHDDWPAVHPLRKDFALRTVLPPFA
                     GERHKFRVVEGEGVFQVPVGPVHAGIIEPGHFLFSVAGESVLYLQIRLFYTHKGTEKL
                     FENLPLAHGVALAESISGDSAFAHATAFCHAIERAAGVEAPPRARAMRSICLELERIY
                     NHIGDIGAIATDVAFVVANAHAMRFKEQVLRLNEQLAGNRLLRGLAAPGGVRSDWNTA
                     QVEALRQRVAWLRPEFESLVALIRESSSTRDRLETTGILRPETARDLGIVGLAGRASG
                     FAHDLRRDFPHAAYADVTFTVPVFQEGDVLRRLQVRVDEVRQSFSIIEQLTSALPSGA
                     IRASLPVLPPDVVALGYVEGWRGEIFHWIRTAPDGRLARCKIKDPSLQNWPALSEAIQ
                     DNIIPDFPVINKSFNLSYSGTDR"
     gene            19010..19846
                     /gene="nuoB"
                     /locus_tag="PKI07_03000"
     CDS             19010..19846
                     /gene="nuoB"
                     /locus_tag="PKI07_03000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOC50049.1"
                     /translation="MFDILYHSLATGVVTTDYPKTPAQTSRQSRGRPEIDAPNWKDAR
                     AAVAACPTGALSCTDGPETRRVTLDLGKCTFCGFCAEADPAIRMSNVCELAARHKAEL
                     TTTIQYQLAPDGTHQALLSPPRAAADPRPAALDAVGAQLKARIAKVLGRSLHIREVDA
                     GSCNGCEVEIAGLNSPVYDLERFGLHFVASPRHADMLLVTGPVSRNMELALRKTYEAM
                     PAPRLVVAVGACGCSGGIFGTNYASLGGVDKVIPVDVYVPGCPPNPYALLHGILTAID
                     RG"
     gene            complement(20026..20487)
                     /locus_tag="PKI07_03005"
     CDS             complement(20026..20487)
                     /locus_tag="PKI07_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOC50050.1"
                     /translation="MPPLGEVEYEAGGERFLRPATLAGVLHALARFPEARLVAGATEL
                     GLDLTKRHRRFSTLVSVEAVPELREIQRTETEWRIGAAATFTQIEELVAGELPALADM
                     LRVFGSRLIRNRAPLGGNLATDSPIGDSAPLLLGSRLRSCWHRSRAANRRA"
     gene            complement(20664..20723)
                     /locus_tag="PKI07_03010"
                     /pseudo
     CDS             complement(20664..20723)
                     /locus_tag="PKI07_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
     gene            complement(20822..21001)
                     /locus_tag="PKI07_03015"
     CDS             complement(20822..21001)
                     /locus_tag="PKI07_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOC50051.1"
                     /translation="MRNAIEFTLNGRRVHVEGVSPSTTLLQYLRHAGLTGAKEGCAEG
                     DCGACAVVFLERDAR"
     gene            21098..22033
                     /locus_tag="PKI07_03020"
     CDS             21098..22033
                     /locus_tag="PKI07_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOC50052.1"
                     /translation="MKRSGLIGMLLLGSALAFARADTLDLADLAQSAEQWAAENLDED
                     VWRLLEDVDREQVEGFFRDLQQRLGGEYVVDLAALNRTAQAVLSLLESRAETQPYAAW
                     LKARLAYLEVAEEFQQSRPAPPRPPDQPAPPPTNAPPKIEREVWTRKLATEPWPRAAT
                     NYVPRLKPVFAEQKVPPELVWIAEVESSFNPRARSPAGAVGLFQLMPETARQYGLRTW
                     PLDHRQRPVDSARAAAKYLASLHKRFKDWRLALAAYNAGEGTVQRLLQKRKARSFDAI
                     ALHLPAETQMYVPRVEATLARREGVKLSQLRFPGG"
     gene            22192..22689
                     /locus_tag="PKI07_03025"
     CDS             22192..22689
                     /locus_tag="PKI07_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50053.1"
                     /translation="MTFDDSPEMARATAAQQLLFPANPRAVSDQVGLNWWTALKLYED
                     GWLSFSPADTPRLDEAQEAELRFLGSLITAGCDRGMLMTLLEQLSKPYAYDLRRLYYD
                     WAERYWRLLPDPLAHPEATFADWLHSLVKQRDVDSLTGILELVQDALSRVRVETAQGE
                     LDRPG"
     gene            complement(22851..23402)
                     /locus_tag="PKI07_03030"
     CDS             complement(22851..23402)
                     /locus_tag="PKI07_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOC50054.1"
                     /translation="MKRWRILAVGLVATLLGVGVVGCRKAAPEAAGTELEPDLPAAAQ
                     PKLQTLNLWLGAEEMVAELALTARQVQTGMMFRTNLAENAGMLFVFSVPHRASFWMKN
                     CPLPLSAAYLDPEGVILEIHDLQPHNTNPVAASSERVQFVIEANQGWFQRHQVKEGMV
                     VRTEHGTLLETFFGNAAKPGGRR"
     gene            24035..25471
                     /locus_tag="PKI07_03035"
     CDS             24035..25471
                     /locus_tag="PKI07_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50055.1"
                     /translation="MNDRTMASSQALPLRNGVARHWSRRQFLRTASAAGVAAPFFLAR
                     TSLLAAPRAISPNSKLAHACVGVGGMGAVDLQRFQAHQRVEIVALCDVDTHALDKAAQ
                     AVPGARLYTDWRELLDKEGGRLDSVNVAVPDHMHFSIAYNAIEQGRHVYCQKPLCHDV
                     AEVRALTEAAVKRGVITQLGTQGASTARERIAVQWLREGRIGKVTHAYLCSNRPGAVE
                     AYRLPGPRPATGQEPPASLKWELWLGSAPRRPYAPEIYHPAKWRAWQDFGTGWSGDIG
                     CHIFDPVWKGLGLKAPRTVWAEVQESWKLSPERRADTWPQGDHIIWTFPGNEKIAGRE
                     LVLEWFDGDYYPPESIRKQYSEDLKEYPPESSFLAGTEGAMLIGHGAVPQLLPEDKFS
                     GIERPRLAERDHYHHFVDACLGGEKTESHFAQSGPMTEAILLGTVAIRVPGQKLEWNS
                     SRLKITNHREANRYLQRRYREGWRVARF"
     gene            complement(25472..26716)
                     /locus_tag="PKI07_03040"
     CDS             complement(25472..26716)
                     /locus_tag="PKI07_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOC50056.1"
                     /translation="MKSAAPVPLHSPGPARLMSLDVFRGATIAAMILVNNPGNWSAIY
                     KPLDHAAWHGWTFTDLIFPFFLWIVGVAIPLSIARRIEQGQSRRQLLLHALRRAVIIF
                     ALGFFLNAFSYLIDGSLFRDGLAAWLHNFATTVRIPGVLQRIGICYFIAILIYLGTNL
                     RGQLLWTAGLLAGYWLMMFVGPWLGWGAGGLAEHGNFCEYIDNLVLNGKTIGTHVWSG
                     GKTWDPEGLFSTVPAIASTLFGVLTGQLLRSRLTPEAKTAWLFVGGNLLLFAGAIMDV
                     WLPINKKLWTSSYAVFMAGMAMNCFAVAYWLVDVHGWRKWARPFAIYGMNAITVFMLA
                     GLLGRISLTIKLTGAGGEKVALKTFLYQHLCAPFASPETAPFFGFLASPQNASLMWAC
                     FYVVGLYLVAYEMYRRKWFLKF"
     gene            26973..30752
                     /gene="smc"
                     /locus_tag="PKI07_03045"
     CDS             26973..30752
                     /gene="smc"
                     /locus_tag="PKI07_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOC50057.1"
                     /translation="MYLKNLTVLGFKSFAEKTVLNFQPGVTAIVGPNGCGKSNVSDAI
                     RWVLGEQSAKALRGGEMADVIFNGTDSRKPLGMAEVSLTVGGVDPEGLAAAGVKLVYD
                     EVTLTRRVFRDGGSEYFINRTPCRLRDIQQLFMGTGVGRTSYSIMAQGNITQILSSKP
                     EDRRMIFEEAAGITRFKAQKKEALRKLEYTEQNLLRIADLVREVKRQIISLQRQAGKA
                     RRYKQLSAELQHLDTQLARHRFDQFQAEIAECQAGVDALRREFEARSAGVLGCEDEIT
                     HLRERLSELEHEISGQQQRGLELKGEMDRHESRIQFNEERLRELAAQDSKAVTDLTQA
                     EERRRGAEADLAEAARQMAAGETALAGHRQAFAARQETLRQVDDELRRQQEALQQAQA
                     GAFAAAQELTRVRNEITALDLQKQGNLARLEKLSAEKIQLEEERGRLEVRLREFEASV
                     TAEKLSAQTQRGTVEQRQERLRQVREELERAAREQDQVLEQQAAKRSRLHVLEQLQAD
                     HEGFSAGSLAALRQSRHVLGSLVDRIRVPDQYVAAIEAALGHHLQLVLTEQPESARQI
                     LAELNTNKAGRASVVPLAFMGPEGNGGGGVAGIAPAALNGRPLPALAVVESEPSIRPL
                     VERLLGSTRLVPDLDAATAAWRETNGQFQYVTLGGELLSSHGVYTGGYNNGFSDGKAP
                     GSLLGRKNQIAELQSGLAALQEQVAEISRNKGALKSEQDELEEGLQQAQTELRAREVA
                     IATHEGEFNALGNSQRVLQQKIDTVVYEIQSLAAQEDEGGRKRAELTAQAGAGEGLAQ
                     SCQARVQELAAHLEALRQKRDQANADLTESRVALAAGEQVYASFQQQQRSLEQRIGEL
                     TQVIEQRRAELSSFVGRREQAESEIQESRGLMDGLRGDREQVNAQLAEGTARKQAQEE
                     ELAAREEGLREQRRHLIGLQEQRGALEVELAQKNMAAQNVREQIQQKYHVNLDTVRSE
                     CITITYADEGPARVHVLTPEEMAAAGAATDWAAVAQQVEALQARLDEMGPVNLVAIEE
                     YEETEERYQFLSGQHEDLVQAKAELMALINRINAQTREMFRETFEKIRESFRTLFVEV
                     FDGGKADLLLMDEGDLLESGIEIVARPPGKQLQTISLLSGGEQTMTAVALLFSIYQVK
                     PSPFCVLDELDAPLDEANINRFIRVLQRFLAHSQFIVITHNKRTIGMADVLYGITMEE
                     HGVSKIVSVKFHKTREEAAGPQAAWMETPAVSPPSAVAPGESPKPDEAHELMLAK"
     gene            complement(30960..31859)
                     /locus_tag="PKI07_03050"
     CDS             complement(30960..31859)
                     /locus_tag="PKI07_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC50058.1"
                     /translation="MKLNRREFLGISVATGAAGVLAPTAQAFTLPDPPQKARLRLSCQ
                     EGVSAGGSLTEKLDFLEANGFEGLEVGGRDLGKRVEELQKALQGRKIKLSAVCAGFDG
                     VLISEQEEVRQKALSSMKEILTAAGALGSVGMIMVPAFHNQTKLGHQEARELLVKLLV
                     ELGEHAHKAGTRVLLEPLNRRECHFLRQVADGAAICRDVNHPGVALMGDFWHMTWEET
                     SDLAAFIAAGKYLQHVHIASRKTRKVPGEDEGDNYIDGFKGLKIIGYQGFVSFECGCK
                     GDRAKALAAAAKLLREQWEQARG"
     gene            complement(31914..32807)
                     /locus_tag="PKI07_03055"
     CDS             complement(31914..32807)
                     /locus_tag="PKI07_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50059.1"
                     /translation="MKTNMTAADKTSKPFQRQRRRGFTLIELLVVIAIIAILAALLLP
                     ALGHAKMRAQGIWCMNNSRQIMIAWQLYAGDHAEWLPPNEDNTDNGNWSGGRMDFDGA
                     NIANYTVAFLIDPKYGKLGPYTKSPGIYKCPADRSTVSMGGHTFNRVRSVAMSQAVGT
                     MRLPPLRSVIAPWLGGDNSQPQLSEPWCKYGKLTDIIRPSPDMLWVVMDEHPDSINDA
                     GLAVECAWTNANAKIIDYPASFHGNACGIAFADGHSEIHKWKDPRTMPPVRYNNDLPL
                     NVPSPNNPDVAWLQMRTSALK"
     gene            complement(32902..35064)
                     /locus_tag="PKI07_03060"
     CDS             complement(32902..35064)
                     /locus_tag="PKI07_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50060.1"
                     /translation="MKLIRIPRLPALFTGFLLLSFALPAVESPYLYGIFDGGDPTPAD
                     ASEYLGRIRNGAGPGWVTVTVSVGANTNDTWGVDYRFLSNQGHTVICRINHGYFPNGT
                     IPPTNSYDAFAVRCASFVANSHGCSIWTIGNELNISGEWPYEPELGKCAYVSPANYAR
                     CFRKVYNAIKAVRPNDKVLPAAPACFAGPFAAGYNSHLGTNYFYDANPLNWVQHLNQL
                     LTAITNTGPNPCPPDGIALHISSRGYTCEDIHSTARFSGGGAGLYKSFYVYKDWVNLG
                     IPRDLYHLPLYATECNGYFYWKGGHPENPFSHYEPGWMQEVYAEISRYNQTAIATGKP
                     IYRCLNMYQWPDGYDGWNINGSSDYFKEDILNDLDEAAAQRHAWPGSIAEALLPVGIN
                     FIHPGSSDDSVPVCGYGYAGVVPQRGWINLTAGGNGANVPLEAGMTITWGAPGGTHEF
                     SHMGNSPADFALMQGYLDTSDSSTTTVTVNGVKFPLYDIIVYGDGDNGSATRVGKYTL
                     TATRGSGTKYILDASDTDFAGSFLEADSPTGGAAAAAGNYCRFRDVRGTTFTVTATGE
                     YASDGHPRGPLNAIQIVPINVVPALSDFSYSQFQFWLNGSTGAAYTIESSSDLTRWTP
                     IGTYIAPTQVAVPGGPQHCFYRAVQTQPYDDVPVLSAPSRRGQVQFSLSGATNMNYII
                     QASTNLVNWTAVSTNRAPARVTLSTGDRRYQFYRALFQ"
     gene            35697..37349
                     /gene="hcp"
                     /locus_tag="PKI07_03065"
                     /gene_synonym="priS"
     CDS             35697..37349
                     /gene="hcp"
                     /locus_tag="PKI07_03065"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOC50061.1"
                     /translation="MFCYQCEQTSRGKGCSTFSVCGKDESSACLQDLLIHASKGIAQY
                     AHRARALGARDASVDRFIVKALFITVTNVNFDADAIAAAVREAAQVRDTAKALYEAAA
                     RKAGRTPETLDGPATWAPAPTLVGLVEQGRTVSILSRTNEYGPDLTGLQELLVYGLKG
                     AAAYADHAQILGQESEIVYAFFAEALCRLAEEPPTADQLLALCLKCGEVNLKVMEILD
                     TAHTSTYGHPVPTPVRVEPLKGKAILVSGHDLKDLELLLQQTEGKGINVYTHGEMLPA
                     HGYPKLKKYKHLAGNFGGAWQDQRDEFRAFPGAILMTTNCLQEPQAGYKERIFTTGHV
                     GWPGIPHVTNGNFGPVIEAALAAPGFPEDGPDKTILVGFGHNAVMSIADKVIEGVKSG
                     AIKHFFLVGGCDGARSGRDYYTEFTESLPKDCVILTLACGKYRFNKLDFGTIGGIPRL
                     LDIGQCNDAYSAIQIAVALSKAFNCGVNDLPLSLVLSWYEQKAVAILLTLLYLGIRNI
                     RLGPTLPAFITPEVLKVLVEKFNLMPIKDAKTDLADILKKAA"
     gene            37897..38958
                     /locus_tag="PKI07_03070"
     CDS             37897..38958
                     /locus_tag="PKI07_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOC50062.1"
                     /translation="MIDSERKLAAYLPELRAATWVAVDTEADSLHAYPEKVCLIQIST
                     ASGDRLIDSLARLNLKPLLEVLAGHELILHGADYDLRLLRRHHKFVPRAIFDTMLAAR
                     LLGQRQFGLCHLVKQYLGVTLEKGAQKANWAVRPLTERMERYARNDTHYLKPLADSLR
                     TELGIRGRLAWHQESCVRLIQDTAAELPPDPDSLWRVKGSHALNRPALAVLRELWHWR
                     EREARAANRPPFFVMSHEAMVQLAATVTVATPSVEPLLPARLSERRRASLKQALEQGL
                     RLPPEQHPHHPRRVNHRVSEGEKRRFSEIQKRRDTRATALGIDPTLIASRALLARLAR
                     DWERHVPELMSWQLELLKA"
     gene            complement(39042..40244)
                     /locus_tag="PKI07_03075"
     CDS             complement(39042..40244)
                     /locus_tag="PKI07_03075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOC50063.1"
                     /translation="MQSLPTVILKPGEADRIVAGHPWIYQSSVIRLTRSAPEGALVQV
                     KDHRQRFLGTGFYHPQSKIAVRMLASDRIEVNQAFFEKRIRAALAVRQKHLPGATSFR
                     VVNAESDFLSGLVIDKYEDVLVIQISSLGMEQRKPLIVAALTAIFSPRAIVERGDAIA
                     RKFEGLPEAHGVLAGLLAGPVPIKLNGLQFEADVLAGHKTGLYLDQQDNYPRVARWAK
                     GAQVLDCFSFLGGFGLHAARAGAAHVHLLEQSAEAVAAATRHAAANGLAELCSFETVN
                     AFDWLKAQTTRSPHEQVIPRWDLIVLDPPSFTRNRASVPDALRGYKEIHLRALKLIKT
                     GGTLATFCCSHHVNSSLFQDTVLSAAFDARRILRRIAVYSQSPDHPIIPMIPETEYLK
                     GFAFEVVR"
     gene            40275..41651
                     /locus_tag="PKI07_03080"
     CDS             40275..41651
                     /locus_tag="PKI07_03080"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOC50064.1"
                     /translation="MQAVINYLKQHQARAIEELCAYVRFPSVSAQPQHRADLRACAEW
                     VVKHCRAIGLQARLCSTAGHPVVVAKTPRAGKPGARRPHFVVYGHYDVQPPEPLDLWT
                     SPPFEPRAARGAIYGRGASDNKGQNLAHLKAVEAYLKTGTPLPCDLTFVIEGEEEIGS
                     SNLTPFLQQYRAELACDAIVISDTGMPSPKHPALTYALRGIAGFEIILRGPAQDLHSG
                     IFGGTVDNPAMALCQLLGKLRDAKGRVAIPGFYDGVRPLSAYERKQFARVPFNTRNYQ
                     RLLGVPKLFGEAGFTPVEQRSARPTFEINGLTSGYQGEGSKTIVPAWARAKATIRLVP
                     DQDPVQVIRCIRRHLAKLCPPSVRLELKERSYAAPYLVSPTGPQAQAALRALKRAFAC
                     EPVLVREGGSIPIVNDFKRILRADALLLGLSLPDDNAHSPNEKFDLGCFAKGQLMSAY
                     LWQELSRT"
BASE COUNT         7587 a        12166 c        13432 g         8516 t
ORIGIN      
        1 gcaacgcttg caacagtcgg cggtgttctt tggggtgttt gccaccgggg ttcgcatcga
       61 gctttgggtt gctggtcggc aactttggtg gttggacttg tcccgttggt tcctagcctg
      121 caggtgacga atgggttctt tttaaggcct tcgtttggtt cacttagact tgaccgtgag
      181 gagatgattt ggtcaacctg ctgcactctg aatgattttc cgctggccgc ttccattctc
      241 ccttatccaa cccggcgcac tgcttaagcg gttgcacagt tcgaccgtgg cctggttttg
      301 ggttttcaat ggtcttcgtt tggccacggg gattctcttg ctgccattgg tgctgcgcaa
      361 gctgtccacg gaggagttgg gcatgtacta tgtgttcctg agtcaggtcg ccctcgctcc
      421 ggtcatagat tttggtttca gcccgacgat attgcgcttt gttagctacg ccatgggcgg
      481 cgcggaaacg atccagcctc agggggtgag caaatccact ggaacgggcc caaacttcgg
      541 cttgctctgg cagttgtttt ttactacccg cgctctttac cgggttctgg cgctggtcct
      601 tctcgtaatc ctgggaatct ggggcaccta catcgtaaac ctgcgcgtcc tcgagacccc
      661 ttgcccgcgc gttacttggg ttgcctgggg catcaccctc attgccacgg tccttaacat
      721 ctattcctcc tgggcgccgg tctttctccg cggcatgaat gaagtcctca tttcggtccg
      781 gattacagtg ctcgcttcgg gggtgaggtt cgcggtcgcg gctgggttgt ttctggcggg
      841 cgcgggtcta atgagcttgc cgctcggaga cctcaccggc agcgttatcc agcaggtgat
      901 cgcccggggc gcctgccttc ggcgattgcg gcgagcgccg tcgacgaaga ggtcggacgt
      961 ttggcaacag ttgcggatta tctggcccaa cagttggcgg ctggggttgg tgaccatggg
     1021 cggttaccta acggtcaatg ccaatacgct gatttgccta aaggttctgg gtttggcggc
     1081 gaatgcacgg tatggacttt cgattcagct cctcgggatt atcagtggca tggcctcggc
     1141 ttggacacag gttaaatggc cgatcatagg ccagcatcgg gcccggcatg attatgccgc
     1201 gatccaacag atcttttggc ctcgaatgtg gctccaatat ttgacgttcc tggccatggc
     1261 ggccggcctt ctggtttgcg gccaaccgct gctgcaattg ctcggcagta ataaacagat
     1321 gcttgcacta ccctggctgg ctttgctggc gctcaactca ttcctggaga tgcaaaccag
     1381 tatctggggc acgctgatta ccacagagaa ccgcatcccg tttctgtggc cttcgttagc
     1441 gtccaaattg ctttcttttg ggctttcgct aacgttcatc tatacgacgt cgttgggttt
     1501 gggggcgttg gttctggcgc cgtttctggc ccattgtctt ttcgattact ggtattggcc
     1561 aattcacgcc gcccgcggca tccagacgtc cttggcgcgt ttcatgtttt cgcggccgaa
     1621 aggcaagccc ggcacgttgt agggtcacga tgcaatcctg ccggcccggc ttgtcttaac
     1681 ggaggatgct gcgaatccag cccactcgac gtttgctttt acgccggccg cgaaaccggc
     1741 tgaatcctgt attcgctggc atagtccggg ccagcgtcac cgctttttca tgatcagaac
     1801 ggcaaactac tttggcaatc gctgcttgct ttagcgccgt cggcgtggaa tgatgaaaac
     1861 gcgttggcgc attgaagcgg gtattcgtgg atatgggcgg gggcgaatta ttccgggagc
     1921 cgtctttgcg ccagtcgtta tccggagtcg tgccatgtgt cggcggatgg cagaagtttc
     1981 ggcattcact ccgctgcaat tctcgcggcg gttgtggcgc gcccccgttg gcaaacgatt
     2041 gctttttacg gacccgctct tactgaacgt ccggcacgga ggcgagccga ggccctcacg
     2101 cggttctgcc cttgccatcc ttgacactca ccacacaaga aatatcctca tcggcatgac
     2161 ctttggcttt agccgattca gcggcaaggc ggcagcttgt ctgctgagac gtttcttgta
     2221 ctgccgggag gaacactaaa ctatgcctgg cccgatctgc gcggcggtag tcttgaatta
     2281 taacggcagg cacctcctgg aggaaaatct gccctcggtc atctcggcgg cgcaaagagt
     2341 caaggaggga tgtgatgtaa tcgtgctcga taatttgagc acggacggca gtgtggagtt
     2401 tttaaagaag aactacccgg gcgtaatagt ggagattgcc ccgagcaacc gaattctgtt
     2461 ctcctacaac gaactctgcc ggcgtattca gtaccagtac gtggccctgc tcaacaacga
     2521 cctgcgcctg tacgaagaca gtctggcggt cctgctgccc cattgcgccg ggcaggatgt
     2581 atttgcggtg tcggcggttt tgttggatgc ggaaggcacg acaatcacgc gcagccggac
     2641 cttgggagaa tcgcaccggg ggttgtttcg agtcgcggcg ttgcaggaaa gtgagaccgt
     2701 gccgaccctg gtcgccggct gctgcgcggt gtatgaccgg gagaaattcg tcgccctcgg
     2761 cggctttgat gaactctact ggcccttcta ctgggaagac aatgatcttt catatgtggc
     2821 ttggaagcgc gggctccggg tgctgattga gccgcggagc gttatgtgcc atcggcacca
     2881 tgctactatc gcatccaatt ctcagaataa agccgtgggc cattgcaatg cccacctctt
     2941 tttttggaag aatatcaccg accctgcact aatcgtgcgg caccttgcct ggctggcctt
     3001 cctcgtcccc tggcgcgcgg tacgaggcca ttggccttac gttcgtggcg tgtgggcagc
     3061 ctgccgtaaa ctgcctcagg ccctgaagcg tcggcgggag atgaaaccgc tgtgggtgcg
     3121 gacggatgcg gaggtttggg cgctggttcg cggcgatgga gaggctgtac ctcccggcta
     3181 gggcggaagc ctggccaaat gcaaaccggc gaacacaggt tcggcggcaa aatgaggccg
     3241 cttagcgtgg cgccgccccg cggagtccga ggggggcagg ttaagttggc cggcttaact
     3301 cataccagga tcgcagcgag gataattgct gctccaggcc gggccgttgc agggtcggga
     3361 gcaacgttcg gtcaattgcc tccttggcca ttgtcgatcg cacttcgatg gggtgctccc
     3421 agattttgct gatgagctga aggatttgac acttggacat tggaggccaa gtgccggctt
     3481 gaatcaccgg ttcgtcggcg cggattaagc cgtttattag tttccaggca atctcagccc
     3541 attccagcgt cgtgataccg ttccacagat ggttggtgta gccattcact tcaccggatt
     3601 gacgcaagaa ccagcccatc aagccccagc ctgtgtccaa ctccggacca atgatggacg
     3661 tgcggataat gaagcatcgg cccgaatcag ccgcggcttc gcccagagct ttggagaggc
     3721 cgtaaacgtc ttccgcgtcg cactcgtcag cgacggagta ctggcctcgc cgccccgaaa
     3781 aaacacaatc actacttgcg tgaatgaggc gttgttcggg gcgcatccgt gcgcggaggt
     3841 gaagggggaa caccgagttg accaggtaaa gttcgccggg gttctccgtc ttttgcttaa
     3901 tccgacccag ggcgttgatg atccaggcgc agcctgaatt ccgaaccgct tcgacgagcg
     3961 aatcccgcgc tgcagcttca tagcgtgcgg atgaagtaat cacctcacaa ccctgttcct
     4021 tcaggaatcg ggccacgacg tggcccagca ttcctttgtg gcccagcaca aaaatcttca
     4081 caagtagaac tcggtttcgc ccatggcatc cagcatggct tggatttcct cggtccgctc
     4141 gagcctttgc gtattggcgg acgtgtactc ctggccgagc ggcttgggag tcagtttttg
     4201 gggcacgcga tactcaggat ggatcgcata ataagacggc tgttccgtca tgcgctgcat
     4261 ttcatactcg ttgacgagga cctcgtggag cttttctccc gggcggatac caactacctt
     4321 gatggggtgg cgtgcccccc gtgggctgta tttcaagcgc atggcttcgg caagtgcctg
     4381 gacggtgcag gcgggcgctt tacggacaaa aatctctccg ccgcgtgcgt gggtcatcgc
     4441 gtgtagaacg agatccaccg attggtcaag cgtcatcagg aaccgagtca tttcggggac
     4501 ggtcaaagtc aagggcgcgc cctggcgaat ctggtcgcga aacaatggga tgacagagcc
     4561 gcggctgccc atcacattgc catagcgcac acaacagaaa gtggtttgat ggtcaaactg
     4621 gttttggctg cagacgatct tttccagcat cgccttgctg atgcccatgg cgttaaccgg
     4681 cttaacggct ttgtcggtgc tcagggccac caggcatttc acgcggttgt acttggccgc
     4741 ctcacaaaca ttctgcgagc cgatgatatt ggttttggca gcctcgtagg ggtagttttc
     4801 gcatgcgggg acctgtttga gcgccgcggc atgaaataca taatccaccc cgttgatcgc
     4861 ttccgccagc cgattggggt ctcgcacgtc tccaacaatg taccggaact gagggaaaat
     4921 cctttgcatt tcccactgtt tcttttcgtc ccggctgtag atcctgattt gggccggttt
     4981 ctgcttcaag aggtaacggg ccacccgatt gccaaaggag ccggtaccac cggtaatcag
     5041 aatgactgag ccagagagat tcttcatttt ccacatattc taacaatctg tcgtggccgc
     5101 agcaatcgcc tttttgccgc acggattcac ccttgggtat tgctcccagc aagagaggct
     5161 ggtgactccc gaaacgacgg gccaatcaaa ccggacattg gcgcatttac atctattcgg
     5221 gttaagcatg ccctgcttag gagccgggtc actctctgtg aatatagtga cgattccatt
     5281 tctttctcac ggaggaggca catgtccgtt caagccccag tgcctggtga caaaatgacg
     5341 gaacgccggc gggagacagt ggaggagcag aattatttct ggcccagcac ctcctgataa
     5401 acctccaggt gccgccggac gattacctcc gggtggtagc gggtacgcat aacttggccc
     5461 gcgccgtgtg cgcgcggaaa gccgctttga atccaatgcg ccagcgcccg ggttaaaccg
     5521 gaccaatcct ccccgtggaa taattccgcg ccgggcactc cctccgcaat atccaccacg
     5581 ccccccacgc gggccgcgaa gagtttcagg tcgcgggcga gtccttctgc caccgctaat
     5641 ccaaaagact ccgcaggcga gaaatgcacc atccccgatg ccgcgtcgaa gagttcaacc
     5701 agttcaaggc tggtttttgt cccgatccac cttgcgcagc cctgtttctc caagggtttg
     5761 aggctttcct ggaaggcgtt tccataggga tggttgggag ttatattgcc gacaaacagg
     5821 aactcgaagg ccaatccctg ctgcgcgagc tcatgcgcta ccttcaacaa ttccaactgt
     5881 cgtttccggg gggcgatcag gcccaggttc accaggcggc accggttgtg gcgggtggga
     5941 actggcgcgg gggcaaagaa ttcctgccgc agcgcatggg ccacgcgcca gacccgccgc
     6001 gttcgcgggc ggaccagttg ttctgtgtag gccgagttgc agagcacgcc cagggttcgt
     6061 ttcagcgcga gagtctccag tcgccccgcc agccagaggt agctgccaac gcgctcctga
     6121 aacagccggg ccaattcagc catattgccg tggattgtga ttacgttggg aaaacccgag
     6181 aagaccgcgc taagggcgca accgtgttcg gtgccatggc catgtacgag gtctggccgg
     6241 atttcccgca gtttctttcg gacggcccgg atgcaccctt ggaagccggt gcgcatccag
     6301 ccaatctttg gcacaaagag gctatggtag aaaatgttgg gggccagctt ttgcggagag
     6361 gtcatgcgca cccgcgcgca ggagaccaca tgcacttcga gttccggcct ctgcttaaag
     6421 ccctccagca gcgcctcggg cgcggtgccg aaatgcggga ccggcgtgcc gtagtccctt
     6481 tgcatgtcgc gattgtcggt ggtcagaaag gcgactttta ttttcaaagc ttcaattcaa
     6541 atttgcctga ctaagggact cattccccgc tggggccgag cgtccaacca ttgagtcagt
     6601 cattgatcat cggttgccgc ggcccgggta cggcctggtc caacttctta ttgccgggcc
     6661 ggaataatcg gcggggttga tgttcgccgc aactcaaata ttgcccgctt gataaacgcg
     6721 gggtgatgca cactccattc ctggccgcac tagagacggc tgcggagcgc atttggttcc
     6781 gcccctttgc ggtttccttg agtttccggg tcagggccag ggttggctgg gatgcttctt
     6841 ttggagtgcg ggcttgagtt tgggataagc gttcgcccgg aaagccggcc aatggcaggt
     6901 ggcttcgagg cgtttgccgt ccggcgcgca gagccagagt ttaacnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnngcatt ggcggagggc tttgtcatcg aaggacggaa
     7081 attccaattc gggcagggcg gccgccacca ggtttacgcg gttgatgaac tgctttgcct
     7141 cgtggttgaa caggggcagc gcgaaccagc ctttctggtg cgcttcggcc aggcagtggc
     7201 cggaagccgc gggatcggtc tcgcgctggt gctcgtggtg gatgatgagg tcgcggaagc
     7261 gaaccagcct gacggcggcg acgcgcttgt gcgcgcggtc gtaaaggtgt tccacccggg
     7321 ccgcgagttg ctccgggaag acttcctcga tccattcgcg ctttacggcc gttgccagtc
     7381 caagcagcgt ccggggctcc gtgccgcgcc cggcgacttc gcgcaggaca gcggccacga
     7441 atagcggggc ggattgcacg acgctctcgc gcatcagcgt tccgcgccgc ccctcggtca
     7501 actcgcattc gagcgttccc tgatcgcgcc ggaggcagag ttggtcaatg aagccggtca
     7561 tgaggcagcg gggcagggcg tcgtctgaaa ccgggctgga cggggagaga ggcggggtca
     7621 ggttatccgc agtctgcgag cccgtcgccg tgtttttgat cagcccttgt tgccgggcga
     7681 tttgcagcaa ttgcgcaaaa gtctgctcca ccagccgcgc cgtttgggcg tggatgccat
     7741 agcggcggca gctttcggcg ctgaacgagt gattccgggc gaactggaag gcacgcatga
     7801 gcgtgtagaa atccgactcc gggctggcct cgaacagttc gcgcgccttt tggaccggtt
     7861 tgtcatcacg gcccaggcgc attagcagat cgcgcccact gaccagggcg gcgcacaaag
     7921 ctgcggacgg cacgcaatcg aaccgggcgg cctcgaccag catgcgcgag tagcgtgggt
     7981 gcattggcag cttgagcatc tgccgtccaa tcggcgtcag accggcagcg ccggaggagg
     8041 ccgcgggccc ttgcgggggc ggttcccgca gtgctcccag cgtaagcagc agttgctccg
     8101 cccgttccac ggcggatggg tcgggtttgt ccagccaatc aaacgcggcg gcgtgctgga
     8161 tgcccaggga atggagcagc agcaccactt cggcgaggtc gctgcgccgg atttccggcg
     8221 tgttgcgttc ggggcggttc aattggccgc tttcggtcca gagccggagg caggtgcccg
     8281 gcgcggtgcg cccggcgcgt cccttgcgtt gctcggccga agcgcggctg atgggctcga
     8341 tgaaaagcgt gttaatgccg cgttcggcgt cgtagcgcgc gatgcgcgcc agcccgctat
     8401 caaccacgtg gcggatgcca tcaatggtga ccgaggtttc cgcgacgttg gtggcgacga
     8461 ccaccttgcg gagggaactg ggcgcgaaag caaggtcctg ctgctcgggc ggcaggtcgc
     8521 cgtgcagggg gatcagtgcc aggcgctcgg ggctgcgaag ggtgcggagg gcggcgaggg
     8581 tggcgttgat ttcggccatg ccgggcatga agaccaggat gtcgccgggg tcgcccgcgt
     8641 tgatgatttg ctcgacggca tcggcggcct gctctgggat tggccgttcg tcccgccggt
     8701 tcaaatagcg caccgcgacg gggaaactct gcccttcaga cagcagaatc ggcgctcctc
     8761 ccgtctcccc tcccaggtat tgcgccaccg gttccgcctc aagcgtggcg gacatgaccg
     8821 tcagcttaag ctcgggccgg tcggtctgct gcaagcgttt taccagggcc agcgcgacat
     8881 cgctcagcag gttgcgctca tggaattcgt cgaagaggac tgcccccaca ttcgatagcg
     8941 tccggtcgtc ttgcagccac cgcaggagaa tcccctccgt gacgtagctg atgcgggtgc
     9001 cgaggctggt ttgatcatcg aaacgaatct ggtagccgac ctccgtcccg agggggcatt
     9061 ggcgctccca cgcaacccgc gccgccaccg tccgtgccgc tacgcggcgg ggttggagca
     9121 cgacaatctt tttgccgccg gccaatccgg cctcgagcag catttgcggg acctgggtag
     9181 tcttgcccga gcctgtcgga gccacgagca ccaatcggtt ccccgttcgc aggatgtgaa
     9241 caatctgcga atggatctcc cggatgggta gtgcgctggg ggtcacagcg tcaggccggt
     9301 ttgcgtgcag cacgggatgg ccagccgtga gttgcggggc ctgccgttgc gcgtccggga
     9361 ggaccggaag gcgggttccg tttgcaagag ccggaggcta atccaaagca ccaggtgcgg
     9421 aggcatagtg tctttagtgg gggattgtag ccggcggaca cggcaaatgc accagcaaat
     9481 ccggaatgcc tcgccccggg cgggctgggg aagtggtgga gccgagggga gtcgaacccc
     9541 tgaccttctc attgcgaacg agacgctctg ccaactgagc tacgacccca ttccactaaa
     9601 tcaggcgaac atactttacg cataagagcc gggcttgcgc aacaaagatt tcaagtggag
     9661 ctagatcgcg aaccgggcga tgcggggcga ggcgttttgc gtcgaattgt ggttcaggcg
     9721 cacccgggga gagtttttca ttaacctttg cgatgcctgg ttgctgcatc ggttgcttgc
     9781 gttggcacgg gacctttgcg aagatcagga agtaggtggg gcgctttatg cgatagaggg
     9841 gattgcaggg cgagctacgg acggcttgca ggtgcgaacg cggttggtgg caaaagcgcc
     9901 ggtgtggcga aatggcagac gcacaggact taaaatcctg ggcccttaaa aaggcgtgtg
     9961 ggttcgagtc ccaccaccgg caccagctcg atttggcatg attgtgtttg ctccgagctg
    10021 cggggccaca aaagaccttc cattgccgat accgctttta ctgccccgcc tgcccaggga
    10081 ttcctgttgg caagatcgcg ccgtgtcatc ttccaggtgc tggttttagg cacaaactat
    10141 ggtccggaag tccaagctgt tcgcgaggag aacgatattg gagtgcggcg gcggctattc
    10201 cgctgctgaa gccgcggagg cggcagcgac cggatttggc cgtggctgct ttcgtcgcag
    10261 tgccagctac tttgctttca ccacccaccc gggtttgact tcggcattgc cggaataaga
    10321 ggctttgctg cgatcggctt ggacagtttg aaggatgacc tcgccgatca gcttttcctc
    10381 cgagaaaacg accgcaccgc tggcatcttt aatgtccacg gtttcgtaga gatcaagctt
    10441 gtcgccgact ttgaatccca ggttgctgcc cagggaaacg atgacggcgt ctttgccggc
    10501 cacggcaagc actttgccgg gggtggcgcg ggcggcttgc gcggcagcct gggcggcggc
    10561 ggcttgctgg ctggccgcgt tggctttttg cttgcgtcgg ccagattcag gcaacgagaa
    10621 ggcacggaca tcttccgtaa tctggttgag cgcggtgacg gtagccttgc ccagggcgct
    10681 atccataaac tccttgttgt cgaatccgat gcccccgccg tggccggtga cgttgacgcc
    10741 cacatcaaag cccgtgcctt tttgggcggc ggtagcggag ccggttttaa tgattttgcg
    10801 cgtggccgca tccacgaggc gccagtcaat gcgcacgtcg gcggtcgttt gtttgagccc
    10861 gaggttgccc aggctgcccg ggacaaagcc gcccaggccg acctttgttt gcttgtttcc
    10921 aaagcgcgtc accttggcgg tgagcataaa atccgccgcc gcaaaacctc ctttttccac
    10981 cttctcgtct tgctgcaccc agccatcctc gcccagcttg atctcatcca tcagcgtgcc
    11041 gagttgggtg ctttcaagca cctggaactt gttgagtttg cccatttcgg tgatgagcat
    11101 ttcgctgagg ccctggccca gagctggctg ccagtactga atggccgtgg tgtcaccgct
    11161 gaacggggcg acgatcaggg acggcagcgt gtcctccgcg gccagagtat atccggtaat
    11221 tcccgccgcc aacatcgcat ggtataaaaa tgccagaagg ttctttttca tgttcatatt
    11281 ctcgctaact tattctttga ctgatcttca agggtgcgcc agcaggacca accatgtcca
    11341 gcttggtccc gcaggctaac cgatgatgct aagctctgcc atagaatgcc cggttgtcaa
    11401 cgggttagtg caaattgata cggctggcac tggcggtacg ccttccgggg tctgctccgt
    11461 cccgaggggc ctgaaaaccg tcaaaaccgc gagggagaca gctgttcggc aggactagtc
    11521 ggctcggggc ccggccaggc tgttagccca ggcaagaatt ttcaaacaaa agtcttgtgt
    11581 cggcaggggc aaacaggtag atagttaggt gtgattttca accactcata ttaggagttg
    11641 taagttaacc ttttctcaca tttatgaata tcattctgca agatttgttg gaactccaga
    11701 cgctggagtt tggcgaagcc accgggaaga atatcgaggc gagagtggcc gcgttgcgcg
    11761 ccaaactgcc gccgcctatt gttggccatt acgatcgcct ccgagttcgg gggaggaagg
    11821 gcatggcaat ggtgcggaat caggtttgca cggggtgcca tatgcgtgtg cctatcggga
    11881 cgatcacgga gttgatgcgg ggcgaagata tccaactttg cgaaagctgc gcccgctatc
    11941 tctacctgcc ggattcagcg aaaaccgagg tgaagaaacc cgaggagacg gcgaaaccga
    12001 aggcgaaagc caaagtaaaa gcaccggcca aaccgcgcaa gcgccgctcg gtgacatccg
    12061 ccgcttaaaa cgccgttaat caggatttaa tgagtcgagc cggcgtgagc tgcacggctt
    12121 gaaggagcat tggactgccc ttgacgctta ttcccctgtg atttcccaaa cgacgattcc
    12181 tgttgtctcc cggcaggctt tcggaaatat ctgccgacgg cagaaatgaa tggcaatgtt
    12241 atagacctgc cgtcctcctt ccttctgcag tgcgccttat gggcctacgt ggcggggggg
    12301 ctgggcagcc tgatcgcatg gcggcgggac cgtcttgcca ccctgattgg gttcggcggt
    12361 gccaccctgg cttcggggtg cggaataagc gcggcgatat tgacgctcgc ggcgtggcca
    12421 gcgtcggcgg ggaaggcgtt tgcactcggg gcttcgggcg ttccctcatt gccgctgatc
    12481 gtccaactgg acccgctcgg ggctttcttt gtgctcatcg tttcagttct ggcactggcc
    12541 ttatccgtgt actcattcgg gtatgtgcga ggatattatg ggcgcaggaa tgtcggtttg
    12601 cttggagcct tctataacgc actgctgctg gcgacgacgc tggtgttcgt cgcgtcgaat
    12661 gcgcttttct ttcttatcct gtgggagatt atggccctga cggcgtattg cctggtcagc
    12721 ttcgagcatg agcgtccgga gacgcgcaat gccggcgtgc ttttctttat catgtcgcac
    12781 cttggcacgg gttgtctgat ccttggtttc ctgctgttat ttcaggcggc gggccaggtt
    12841 ctgggcaatt atggtccgga ctcatactct tttgaaagtt ttcgcgcgtt gggcaatagc
    12901 ctgccgcccg ggccgcgaaa tgccgccttt ttgctgttcc tggtcggttt tggagtgaag
    12961 gccggcattg tgccgttgca tatttggctg ccggcagctc atccggtggc gcccagcaat
    13021 gcatcggcct tgctgtccgg ggtgatgatt aaaaccggca tttatgggct aacgcgcgtg
    13081 ctgttcgact ttatgggcac gccgccgaac tggtggggcg tgaccctctt gacgttggga
    13141 accctgtccg cggtgctggg ggtgctttac gcgctcatgg agcacgactt gaagcggttg
    13201 ctggcgtata gcagcattga gaatatcggc atcatcctga tggggttggg cgcggccttg
    13261 atgtttttgc atacgcagca tccggtgctg gcgtcgctgg cgctcatcgc ggggctctat
    13321 cacacactca accatgcgtt cttcaaggcg ctgctgtttc tgggggcggg cgctgttcag
    13381 catgcgttgc atacacgcaa catggaggaa atgggaggcc tgataaaacg gatgccgcag
    13441 acggctttct gcttcctcct gggggccgtg gcgatctcgg ctttgccgcc gttgaatggc
    13501 tttgtcagcg aatggctcac ctaccagtca ctgctgcaag gtttcgggac tacgggcagc
    13561 ttggtgaagc tggtgtttcc cttgagtggc gccatgctgg cactgacggg cgcattggcg
    13621 gcggcatgct tcgtgaaggc gttcggcatt acgttcctgg ctcaaccgcg cagcgaaccg
    13681 gcagcgcagg cgcgcgaagc gtctccggcg atgctgtccg ggatggggct gctcgctgcc
    13741 ggttgtgttt tcctggggct tttcccgacg gccttgatca ccctgtttgg gccgctgaca
    13801 caacaactgc tgggccagcc gttgcccggg taccttgtgg ccggccacgg ttggatactg
    13861 ggcaacacgc aggaactggg gggaacggtc tccacgccgg gcatcgtgct tgcggggatt
    13921 tgcctgttgc cggtgccgct ggtgttgtgg ctgttctttg ggcggcgaac gcgggtgcgc
    13981 atcggcccga cgtgggactg cggactgcga ggattgacgc cgcacatgca atatagcgct
    14041 actggcttct ccaaaccctt gcgggtaatc ttcaaagccc tgttccggcc ccggcgcgag
    14101 atgcagcgag agtatgatta ctcgccgtat tttgccacca ccctgcggtt cgagagtcgg
    14161 attgaggaag catttgtcac tcgcctttat cggcctctga aacggcgcat cctcctgacc
    14221 tcccgtcgca tgcgcgcgtt gcaggcgggg agcatacagg cctatctcat ctacattttc
    14281 gtcacgctgc tgctgctgct gatatttgcc ttgtgaagat tgcgctggcc attctgttgc
    14341 agacagtcct gctgctgggg cttgcgcctt ttatcagcgg ttgcatcaag actctgaagg
    14401 cgaaccttca aaaccgccgc gggccgcgcg cctggcaacc gtatttggac ctgtggaagt
    14461 tcctgcacaa ggacatggtc atctctgagc acgcgtcctg gatttttcga gcgacgcccc
    14521 aggtgttgtt cttcaccacg ctgctcgccg gcctgatgat gcccctggtc agcgtctccg
    14581 cgccgctgag cctgttcggc ggcgtattgg cgtttgccgg attgctggcg ctggggcggt
    14641 tctttctggc gcttggcggg ctggaccccg caagtgcttt cggcggtatg ggcagcagcc
    14701 gcgagatgac gatggcggca attgccgaac cggccttgat gctggcaatc ttcactgtgg
    14761 ccattgaagc ccgctccacc aatctcagcc agatgctatt gtccgcccag gccccgacct
    14821 ggcaattgct taaccccgcg cacgtgctgg cctttgccgc cctgtttatc gtcctgctgg
    14881 cggaaaccgg ccgtattcca gtggacaatc ccgcgacgca cctggagctg acgatgatcc
    14941 acgaagccat gattctggag tattcggggc gctacctggc gctgatcgaa tggagcgctt
    15001 cgatcaaaca gcttttgctg atggcgctgt tgttgaacat ctttttcccc gtcggcatca
    15061 ccgcagatct gagcgtgggc gcggtagggc ttgcgctgtt ctggtttgtg gccaagctgc
    15121 tggcgctttc ggccctggtg gtattggtcg aaaccacgaa tgccaagtta cggctgtttc
    15181 gcgtgcccga tttgttgagc gcggctttta tcctgggcgc gctggccctg ctttccacct
    15241 tcctttttca atgaagccgc tcaacccgga atttgcctcg caatggatca ctctgctcgc
    15301 aaccgggatg ctggtggtgc aattgctgat ggtggtgcag cgcatgctgc tgaccaacat
    15361 ccggctcttt gccctgcagt cgctgatgct ggcgggcatc gcggtcatca tcgccttttc
    15421 ccacgcccat gccgcgcatg tctattgggt ggcaggcctg acgctggtgg gcaaagtctt
    15481 ctttctgccg tggctgctga gccgccaggt gcggcgcatt caaatggaac atgagattgc
    15541 ccccctgctg aatgcgtccg cttcaatgct gttgtgcggg gcgtttactt tgctgggcta
    15601 cattgtcgcc cgtcccttca cttctctggc ccggttgggc aataatacgc tgggcgttgc
    15661 cgtgacgttg ctcttgaccg ggtttttcct catggtcaac cgccgcaaag cccttaccca
    15721 ggttctcgcc ctgttgaccg tcgagaacgg cgtcatgctg gcggcggttg ccctgaccac
    15781 ctacggcatg ccgctggtgg tcgaactggg catcttcttt gacctcatgg tcgcggtgat
    15841 ggtgctgggc ctgctggtct atcgcattcg cgagacgttc tcctcgatgg acgtcagcaa
    15901 actgagccaa ctgaaaggat gacgccgttg gttcccattc tggtagtgcc gacggcggcg
    15961 gggctgctct gcctgctgct gcattcgcgg cgcgcgatgg cgctggcgaa cgggctggct
    16021 tttacggtga cgctggggct gagcgtgtgg ctgctgctgg cgatgcggcc cgggcctcag
    16081 gtggtgaccg agtgcaatga gttcttccgc gccgacgcgc tgagcgcgtg gatggccctg
    16141 ctgatttcgg tggtttccct cgggagttcg atttatgccg ggcgctattt ccagcgcgat
    16201 ctggcggcgg gggccgtgac gccgggccgg gtgaaagagt tctatgtgct gacgccgctg
    16261 ttcggcgcgg gcatgtttct ggtggtgctg gccaataacc ttggggtgat gtggctggcg
    16321 ctggaggcca cggcgttatc ctcggtgctg ttggtggcgt tgtacaatcg cagcacctcg
    16381 ctggaggccg cctggaaata tgtgatcctg ggcagcctgg ggctggcgct cgcgttattt
    16441 ggcacggtct tcacttacgc cgcggccatt gatcagcagt cggcggttga gttgcccagc
    16501 ttcaactggt cgcacctgat gtccatctcc ggccagctca acccgcacat gatcaagctg
    16561 gctttcgttt tcgtgttggt gggctatggc accaaagccg gactggcccc catgcataac
    16621 tggctgccgg atgcgcacag cgaggcgcct tcgcccacca gcgcgatgct ttccggcgtg
    16681 tcgttgaaag tcgccctcta tgctttggtg cgatttcata ttttaaccac ggcttccctg
    16741 ggcacgtcgt tcagccagac gctcctgttg gtgtttggcg tgacctcgat gtgcgtggcg
    16801 gctcccttca ttctcgtgca gtccaatctg aagcggctgc tggcttattc cagcctggaa
    16861 cacgtggggc tgatctgcgc ggggataggg cttaattccc gcatcaccat ctttggcgcg
    16921 ctgctgcaca tggggtatca cgcgctggcc aaaccagtgc tgtttttcgc ggccggcaac
    16981 cttcatcaga cctgccacac gctgcaactg cggcggattg gcccgggcat ggcgcgggtg
    17041 ctgccgctga cggcgttttg cctgggatta gccggagtgg cggcggtggg actgcctcca
    17101 tttggcttgt tcttcagcga gattctggtg ttgagcggcg ggtttgccgc ggggcatctc
    17161 acggcgaccg tgcttctgct ggtggcgatc ctcgcctcgt tttgcgggct gctttaccag
    17221 tttgcgcgca ttttgccggg cgaaccgaaa gtcgcgcgca ggagcgtgcc cgctcccctg
    17281 gccggagcgc cggcgatgct tctgctgctg ggggcgttgc tggtgttcag cgtgtggctg
    17341 ccggctccgc tgctcgaagt catacggcag gcgaccgaca ttatcggagg agcaccatga
    17401 acgcactccc ggaactgcaa cccatcctgg caagtttgac cgagcgcttt ggcgcgcaaa
    17461 tccagcccat gcagatcccg cgccccaacg aagtttattt ccagggcaaa atggagctgg
    17521 tgcccggctt ttgcgcgttg ctttacaagg aatggggggc gcggctggcc agcctgtttg
    17581 cggatgatgt ccgcgcccaa accggggatt tccacctgta ttatgtgttt gcgctggacg
    17641 cggcgcatgg ctttctcatc ctgcgcgtcc cggtgccgcc atcccagccg cggttcgtct
    17701 ccctgacgaa tgcgctcccg gcgctcaact ggcaggagcg ggaaatccag gacctgttcg
    17761 gactcaagct cgaaggccat cccaacgcgc gcccttgcgc gctgcatgat gactggccgg
    17821 cggtgcatcc gctgcgaaaa gattttgctt tgcgcacggt gctgccgccc tttgccgggg
    17881 agcggcataa gttccgggtg gtcgaaggcg aaggcgtctt ccaggttcca gtgggcccgg
    17941 tgcatgcggg catcatcgag ccgggccact ttttattcag cgttgccggg gagtccgtcc
    18001 tctacctgca aattcggctc ttctacaccc acaagggcac ggagaagctt tttgaaaacc
    18061 tgccgctggc ccacggcgtg gccctggcgg agagcatctc cggcgattcc gcctttgcgc
    18121 acgcgacggc cttttgtcac gccatcgagc gggcggcggg ggtggaggcg ccgccccggg
    18181 cgcgcgccat gcgcagcatc tgcctggaac tggagcgcat ttacaaccat atcggcgata
    18241 ttggcgccat cgcgaccgat gtggccttcg tcgtcgccaa cgctcacgcc atgcggttca
    18301 aggagcaggt cttgcgcctg aacgagcagc ttgccggcaa ccggctcctg cgcggcctgg
    18361 ccgccccggg cggcgtccgc tccgactgga ataccgccca ggtcgaagcc ctccgccagc
    18421 gagtggcctg gctgcggccg gagttcgagt cgctggtcgc tttgatccgc gaatcttcct
    18481 ccacgcgcga ccgcctggaa accacgggga ttctcaggcc ggagaccgcc cgcgacctgg
    18541 ggattgtcgg gctggcgggc cgcgcctcgg gctttgccca tgacttgcgc cgcgacttcc
    18601 cgcatgccgc ctacgcggat gtgacgttca cggtgccggt cttccaggaa ggcgacgtgc
    18661 tccggcgcct gcaggtccgc gttgacgaag tgcggcagtc gttttccatc atcgaacagt
    18721 tgacgtccgc cttgccttcg ggcgcgattc gggcaagcct gccggtgctc ccccccgatg
    18781 tggtcgccct gggctacgtc gagggctggc gtggggagat attccattgg attcggaccg
    18841 ccccggacgg ccgcctggcc cggtgcaaga tcaaggatcc ctccctgcaa aactggccgg
    18901 cactcagcga agcgattcaa gataacatca ttcccgattt cccggtgatc aataagagct
    18961 tcaatctgtc ctattccggc acggaccgct gaccccgccc cctccgccca tgtttgacat
    19021 tctctaccat agtttggcta cgggcgtcgt gaccacggat tatccgaaga ctcccgcgca
    19081 gacgagccgc cagtcgcgcg gccggccgga gattgacgcg ccgaactgga aggacgcgcg
    19141 ggctgcggtt gccgcgtgcc cgaccggggc cttgtcctgc acggacggcc cggaaacccg
    19201 ccgcgtaacg ctggacctgg gcaaatgcac gttttgcggc ttctgcgcgg aagccgatcc
    19261 ggccattcgc atgtccaacg tgtgcgaact ggcggcccgg cataaagcgg agttgacaac
    19321 cacgattcag tatcagctcg cgcccgatgg cacgcaccag gcgttgcttt ctccgccgcg
    19381 cgccgcggct gatccccggc ctgcagcgtt ggacgcggtc ggcgcgcagt tgaaggcgcg
    19441 cattgctaaa gtactgggcc gctcgctgca cattcgggaa gtggatgccg gttcgtgcaa
    19501 tggctgcgag gtcgagattg ccggcctgaa cagcccggtc tatgacctcg aacgtttcgg
    19561 ccttcatttt gtggcctcgc cgcgtcatgc cgatatgctg ctggtaaccg ggcccgtttc
    19621 ccgcaacatg gaattagcgc tgcgcaaaac gtatgaagcg atgccggctc cgcgcctggt
    19681 ggtggccgtg ggcgcctgcg ggtgcagcgg aggcattttc ggaacgaact atgccagcct
    19741 gggcggcgtg gataaggtca tccccgtgga tgtctatgtc cccggctgcc cccccaatcc
    19801 atacgccttg ctgcacggca tactgacggc gattgaccgc ggctagacgg cagggcttct
    19861 ggcggcgggt tacccggcat tgtgggccgg gccttgaatc cctgcctgcg gagatgagcc
    19921 gccccgcgcc gcgcggcacg cgaatggtct tgagcatctc gccgaggcgc agggcggtct
    19981 ggcgataagc gacaaagaac tccgccagcg ggagggtgcg ctcgatcagg cgcgccggtt
    20041 tgccgcgcgc gagcgatgcc agcacgacct gagcctcgag cccagcagca ggggggcgct
    20101 gtcgccgatg ggggagtcgg tggccaggtt gccgcccagg ggggcgcggt tgcggatcag
    20161 gcgcgaacca aaaacgcgca gcatgtccgc cagcgcggga agttcgcccg ccactagctc
    20221 ctcgatctgc gtgaaggtgg ccgcggcgcc aatgcgccac tccgtttccg tgcgctggat
    20281 ctcccgcaat tccggcacgg cctcgaccga gaccagggtg gagaatctcc ggtggcgctt
    20341 ggtgaggtcg agccccaact ccgtggcccc ggccaccagc cgggcctcgg ggaagcgcgc
    20401 cagcgcgtgg agcacgccgg ccagggttgc gggccggagg aacctttccc cgcccgcctc
    20461 atattcgact tcccccaggg gcggcaccgg cttccgcaac cggccggcga agccatcccg
    20521 cccggcgggg atgcgccccg gcccgaaagc cgctccggcg gcctggcgaa tcgggcggca
    20581 gccggtgcag cggcagagat tgcgcttaat tggtcgtcca gttggccggc ctgccgaata
    20641 tcgccgcggt agtacccctc gaagagcgac atcacaaacc ccggcgtgca atacccgcat
    20701 tgcgagccgt ggcgccggac catctgttcc tggacggggt gcaaccggct gccggccgcc
    20761 actccttcga cgctgaggat ctcgcgcccc gccatgaggc agaaggggac caggcaactg
    20821 ttcagcgcgc gtcgcgttcc aggaacacca cggcgcaggc gccgcaatcc ccctcggcgc
    20881 agccttcctt ggcgcccgtc agcccggcat ggcgcaggta ttggagcagg gtggtgctgg
    20941 gggagacgcc ctcgacgtgg acgcggcggc cattgagagt gaattcgatg gcgttgcgca
    21001 tagctggttg aataacgcta aagcagaccc cggaggctgt aaatcaaata ggaagagatt
    21061 gctgcgccgg gcgcgccggt gtggtaatct cccggcggtg aaaagaagcg gcctcatcgg
    21121 gatgttgctg ctgggttccg ccctggcctt tgcccgggcg gacaccctgg acctcgccga
    21181 cctggcgcag tcggcggaac aatgggccgc ggagaacctc gacgaggatg tctggcgcct
    21241 gctggaggat gtggatcgcg agcaggtgga aggatttttc cgcgacctgc agcaacggct
    21301 gggcggcgag tacgtcgtgg acctcgccgc gctcaaccgc accgcccaag ccgtcttgtc
    21361 gctgctggag agccgcgccg aaacccagcc gtatgccgcc tggctcaaag cgcggctcgc
    21421 ctacctggag gtggccgagg agttccagca gagccgcccg gctcccccgc gcccgccgga
    21481 ccagcccgcg ccgccgccca ccaacgcgcc gccgaaaatc gagcgtgaag tctggacccg
    21541 aaaactcgcg accgagccct ggccccgcgc ggcaaccaat tacgtgccgc gcctcaagcc
    21601 cgtttttgcg gagcagaaag tcccgcccga attggtgtgg atcgcggagg tggaatcctc
    21661 cttcaacccc cgggcgcgca gccccgcggg ggcggtgggg ctgtttcagc tcatgcccga
    21721 gaccgcccgc caatacgggc tgcggacctg gccgctggac catcggcagc ggcccgtgga
    21781 cagcgcgcgg gcggcggcca aatacctggc cagcctccat aagcggttca aagactggcg
    21841 gctggcgctc gcggcttaca acgccgggga gggaaccgtc caacggctgc tgcaaaagcg
    21901 caaggcgcgg tcgtttgacg ccatcgccct gcacctgccg gcggaaacgc agatgtacgt
    21961 gccccgcgtc gaggccaccc tcgcgcgccg cgaaggcgtg aagctcagcc agctccgctt
    22021 ccccggcgga taggcgcaag ccgggccccg gcgggccgaa aaaagattgc gaaaagcggc
    22081 ttcccttttc tccgcgccgt ggataatatg ctggcggaca agatgttagg ggcagcggat
    22141 tcagaccggc ggaatcggga aatgcccggc tgacggctgc ccggcgtgca tatgacattc
    22201 gatgacagtc cggaaatggc ccgcgccacc gccgcacagc aattgctgtt tcccgccaat
    22261 ccgcgcgccg tctctgatca agtggggctg aactggtgga cggccctgaa gctctacgag
    22321 gatggctggc tcagtttttc ccccgccgat acgccgcgcc tggacgaagc gcaggaagcc
    22381 gaactgcgtt tcctgggctc actgatcacg gccggctgcg accggggcat gctcatgacc
    22441 ctgctggagc aactgtccaa gccctacgcc tacgatctgc gccgccttta ctatgactgg
    22501 gcggaacgct actggcggct cctgccggac ccgctggccc atccggaagc cacgttcgcc
    22561 gactggctgc acagcctggt gaagcagcgg gatgttgatt cgctgacggg catcctcgaa
    22621 ctggtccagg acgcgctctc ccgggttcgc gtcgagaccg cgcaaggcga actggatcgg
    22681 cccgggtgag cccgcttccc cgggaaccgg cagtgctccc gcccgcggca tttgcccgcc
    22741 ccgccgaccg gacagggcag ccagaacttc acaaccggcc cggtgcctct ggccggaagc
    22801 ccttggctcg tattccccgc accggcctgc ggctgcggag ctctccacga ttatcgccgt
    22861 ccgcccggct ttgcggcatt gccaaagaat gtctccagca aagtgccgtg ttccgtgcga
    22921 acgaccattc cctccttgac ctggtgccgc tggaaccatc cctggttggc ttcgatcacg
    22981 aattgaaccc gctctgagga ggccgcaacc ggattcgtat tgtgcggctg cagatcatgg
    23041 atttccagga tgacgccttc cgggtcaagg taagccgctg agagcggcag cggacagttt
    23101 ttcatccaaa aggaggcgcg atggggaacg gagaagacga acaacatgcc ggcgttctcc
    23161 gccaggttcg tgcgaaacat catgccggtt tgcacctgcc gggccgtcaa cgccagttca
    23221 gccaccattt cctccgctcc cagccaaagg ttcagcgtct gcaatttggg ttgcgctgcg
    23281 gcgggcaaat ccggctccag ttcggttccg gccgcctccg gcgcggcctt ccggcacccg
    23341 acgacaccaa cccccagcag cgtcgccacc aacccaaccg ccaggatgcg ccatcgtttc
    23401 atcgccccaa cctgagcgaa agtcacgcgc cttgcaagcg ccacttctct tttggcagtg
    23461 ccgccaccac ctgccgaact gtgccaattt ggcacacctc aacctcggca aaagcaccca
    23521 tcctcatttc cggcctgcct ccgtccctcc atccgtaaaa atctgaaaaa agcccaccca
    23581 cccaccggaa ctgcccgcaa tcctgccagc cagtcgtcct cccgcccata tcccaattcc
    23641 cgccaatatc cgtcattcct ccctcccaaa gcgctgtgta caaggggtgt acaagggatg
    23701 ctcatcgggt gtaccagcta aaatcctatg gactcccggt gagcatccct tgtacacccc
    23761 ttgtacacag cgttttagga gggggaaatg ggcttggcag tggctaagtt tctcaaaagt
    23821 gagaatagaa gcggcgggag atggcgaatg atcgctgaga agcctttcag cgggagtgtc
    23881 caataattgg acagttcgag atagcgtcgg gaggctggga agtgggcagg gctgcggact
    23941 attgaggagg gcaggattac ggatttgggg tggggggcga gaaagactca ttgaagggct
    24001 tgcctttggg tcggctttct cacagtttgc ctgcatgaac gaccggacaa tggcttcctc
    24061 tcaggcactc cccttgcgaa acggcgtggc gcggcactgg tcgcgccgtc aattcctgcg
    24121 gacggcgtcc gcagcgggtg ttgccgcgcc gttctttctg gcccgcacca gcctgctggc
    24181 ggcgccccgg gccatttccc ccaacagcaa gctggctcac gcctgcgtgg gtgtgggcgg
    24241 catgggagcc gttgatttgc aacggttcca ggctcatcag cgggtggaga tcgtggcttt
    24301 atgcgacgtg gacacgcacg cgctcgataa agcggcccag gccgttccgg gcgcgcggct
    24361 ttacaccgac tggcgggaat tgctcgacaa ggaggggggc cggttggatt cggtgaatgt
    24421 tgccgtgccg gaccacatgc atttctcgat tgcctataac gcgattgagc agggccggca
    24481 tgtctattgt cagaagcctc tatgccacga tgtggcggag gtgcgcgcgc tgaccgaggc
    24541 ggcggtcaag cggggcgtga tcacccagtt gggcacgcag ggtgcctcga cggcgcggga
    24601 gcgcatcgcg gtgcagtggc tccgagaagg gcggatcggc aaggtgacgc atgcctatct
    24661 ctgctcgaac cggcccggcg cggtcgaagc gtatcggctt ccggggccgc gcccggcgac
    24721 gggccaggaa ccgcccgcct ccttgaagtg ggagctttgg ctgggcagcg ccccccgccg
    24781 gccttacgcg ccggagattt accatccggc caagtggcgc gcgtggcagg acttcggcac
    24841 cggttggtcg ggggatattg gctgccatat cttcgacccg gtgtggaaag ggctggggct
    24901 gaaggcgccc aggaccgtct gggcggaagt gcaggagtcg tggaaacttt ccccggagcg
    24961 ccgggcggac acctggccgc agggcgacca catcatctgg acgtttccgg gcaatgaaaa
    25021 gatcgcaggc cgggagctgg tgctggagtg gtttgacggc gattactatc cgccggaatc
    25081 catccgcaag cagtattcgg aagatttgaa ggagtatccg ccggagtcct cgttcctggc
    25141 cggaacggaa ggcgcgatgt tgatcggcca cggcgcggtg ccgcagttgc tgccggagga
    25201 caagttcagc gggatcgagc ggccccggct ggcggagcgc gatcattacc atcacttcgt
    25261 ggacgcgtgc ctgggcggcg agaagaccga atcgcacttt gcgcagagcg ggccgatgac
    25321 cgaggccatt ctgctgggga cggtggccat ccgggtgccg gggcagaagc tggagtggaa
    25381 cagttcccgg ttgaaaatca ccaaccaccg ggaagccaac cgttatcttc agcggcgcta
    25441 ccgcgagggc tggcgggtgg cgcggttctg atcagaactt gaggaaccac ttgcgccgat
    25501 acatctcgta ggcgaccagg taaagcccca ccacgtaaaa gcacgcccac atgagcgagg
    25561 cgttttgggg gctggccagg aagccgaaga aaggcgcggt ctcggggctg gcaaacggcg
    25621 cgcagaggtg ctggtaaagg aaggttttga gggccacctt ttcgccgccg gcgccggtca
    25681 gcttgatggt gagggagatg cgccccagca ggcccgccag catgaacacc gtgatggcgt
    25741 tcatgccgta gatggcgaac ggccgggccc acttgcgcca gccgtgcacg tccaccagcc
    25801 agtaggcgac cgcaaagcag ttcatcgcca tgccggccat gaacacggcg taggaactgg
    25861 tccagagttt cttgttgatc ggcagccaca catccatgat cgccccggca aacagcagca
    25921 ggttgccccc cacgaagagc cacgccgttt tggcttccgg cgtcagccgg gagcggagca
    25981 gttggccggt caggacgccg aacagggtgg aggcaatcgc gggcaccgtg ctgaagaggc
    26041 cttccgggtc ccaggtcttg ccgccgctcc agacgtgggt gccgatggtc ttgccgttca
    26101 acaccaggtt atcaatgtat tcgcagaaat tgccgtgttc ggccaggccc ccggcgcccc
    26161 atcccagcca tggcccgacg aacatcatca gccagtagcc ggccagcaaa cccgccgtcc
    26221 agagcaattg gccgcggagg ttggtgccca ggtaaatcaa tatcgcgatg aagtagcaga
    26281 tgccgatgcg ctgcagcacg cccgggatgc ggacggtggt ggcaaagtta tgcagccagg
    26341 cggccaggcc gtcgcggaaa agcgagccgt caatcaggta gctgaaggcg ttcaggaaga
    26401 agcccagggc gaaaataatc accgcgcggc gcagcgcgtg gagcaggagc tggcggcggc
    26461 tttggccttg ctcgatccgg cgcgcgatag aaagcgggat ggcgacgccg acgatccaaa
    26521 ggaagaacgg gaagatcagg tccgtaaaag tccagccgtg ccacgcggcg tggtcgaggg
    26581 gtttatagat ggcgctccag ttgcccggat tgttgaccag gatcatggcg gcaatcgttg
    26641 ctccgcgaaa gacatcgagg gacatcaggc gggcggggcc agggctgtga agcggcacag
    26701 gggcggcgga tttcatattg aatcgcgggc agactacggg gattccgggc ggaaggaaag
    26761 cggctttccc gcaaataata gttgccagtt ttgctgggat ggggcaaaat aagcatcgaa
    26821 gaccattgca taggctttag atagcggtta gcagcacaaa accaatctcg gttcctcacc
    26881 aattagttgt gctccgacag cgatggtatc ctgtatattg tggtttttag cctttactag
    26941 gcagagcgag tgtggttggc tttggcaaga tcatgtatct gaagaacttg acagtcctgg
    27001 gatttaagtc ttttgcggag aagactgttc ttaatttcca gccgggggtc acggccatcg
    27061 taggccccaa tgggtgtggt aagtccaatg tgtcagacgc tatccgttgg gtactgggcg
    27121 agcaatccgc caaggccttg cggggcggcg agatggcgga tgtgattttt aacgggacgg
    27181 acagccgcaa gccgctgggc atggcggagg tgtcgctgac ggttgggggg gtggacccgg
    27241 aagggcttgc ggcggcgggc gtgaaactgg tgtatgacga ggtgaccctt acccggcggg
    27301 tcttccgtga cggtggcagc gagtatttta ttaatcggac gccttgccgg ttgcgggata
    27361 tccagcaact gttcatgggc accggcgtgg ggcgcaccag ctatagcatc atggcgcagg
    27421 gcaacatcac gcaaatcctc tccagcaaac cggaggatcg gcggatgatc ttcgaggagg
    27481 cggcgggcat tacccggttt aaggcccaaa agaaagaggc gctgcgcaag ctggaataca
    27541 ccgagcagaa cctgttgcgc atcgccgacc tggtccgcga ggtgaagcgc caaattatct
    27601 cgctccaacg ccaggccggc aaggcccggc gctacaagca gctctccgcc gagcttcagc
    27661 atttggatac ccagctggcg cgccaccggt ttgaccagtt ccaggccgag atcgccgagt
    27721 gccaggcggg ggtggatgcg cttcgccgcg agttcgaggc ccgttcggcc ggcgtgttgg
    27781 gctgcgagga cgaaatcacg catttgcgcg agcggttatc cgagctggag catgagatca
    27841 gcggccagca gcagcgcggg ctggagttga agggggagat ggaccggcac gagagccgga
    27901 ttcagttcaa cgaagagcgt ttgcgggagc ttgccgcgca ggacagcaaa gccgtgacgg
    27961 accttacaca ggccgaggag cggcgccgcg gcgcggaggc ggacctggcg gaggcggccc
    28021 gccagatggc cgcgggcgaa acggctttgg ccgggcatcg gcaggcgttt gccgcccggc
    28081 aggaaacgct gcgccaggtg gacgacgaat tgcgccgcca gcaggaggcg ctgcagcagg
    28141 cgcaggccgg ggcctttgcc gcggcgcagg aactgacgcg ggtgcgcaat gagatcaccg
    28201 cgctggattt gcagaagcag ggcaacctgg cgcggctgga gaagctgtcg gccgagaaga
    28261 ttcagttgga agaggagcgg gggcggctgg aggtccggct gcgcgagttc gaggccagcg
    28321 tgacggcgga gaagttgagc gcgcagacgc agcgcgggac ggtcgagcag cggcaggagc
    28381 ggctgcgcca ggtgcgggaa gaactggagc gcgccgcgcg cgagcaagac caggtgttgg
    28441 agcagcaggc ggcgaaacgc tcgcgcctgc atgtgctgga gcagcttcag gccgaccacg
    28501 agggtttcag cgccggatcg ctggccgctt tgcggcagtc ccggcatgtg ctgggttcgc
    28561 tggtggaccg catccgcgtg cccgaccagt atgtcgccgc catcgaggcg gccctggggc
    28621 atcatttgca actggtgctc acggagcagc cggagtcggc gcggcagatc ctggcggagc
    28681 tcaacacgaa caaggccggc cgggcgagcg ttgtgccgct ggctttcatg ggcccggagg
    28741 ggaacggcgg gggcggggtg gcgggcattg cgccggccgc gctgaacggc cggccgctgc
    28801 cggcgctggc ggtggtcgag agcgagcctt caatccgccc gctggtcgaa cgattgctgg
    28861 gctcgacgcg gctggtgccg gatttggatg cggccaccgc cgcctggcgc gaaaccaatg
    28921 gccagtttca atatgtcacc ctgggcggcg agttgctgag ttcccacggc gtttacaccg
    28981 gcggctacaa caacgggttc agcgacggca aggcgccggg ctcgcttctg gggcggaaga
    29041 accaaatcgc cgagctgcag agcggcctgg cggccttgca ggagcaggtg gcagaaatca
    29101 gccggaacaa aggcgcgttg aagagcgagc aggatgaact cgaggagggc ctgcagcagg
    29161 cgcaaacgga actgcgcgcc cgggaggtgg ccattgccac gcacgaaggc gagttcaatg
    29221 ccctcggcaa ttcgcaacgg gtgctgcagc agaagattga tacggttgta tatgagatcc
    29281 agagcctggc ggcccaggag gacgagggcg gccggaagcg ggcggagctg accgctcagg
    29341 ccggcgccgg ggaggggctc gcgcaaagct gccaggcgcg ggtgcaggag ttggcggcgc
    29401 acctcgaggc actgcggcag aagcgcgacc aggcgaatgc cgacctgacg gaaagccggg
    29461 tggccctggc ggcgggcgag caggtttatg cttccttcca gcagcagcag cgttcactgg
    29521 agcagcgcat cggtgaattg acgcaggtca tcgagcagcg gcgcgccgaa ctttcctcct
    29581 ttgtgggccg ccgggagcag gcggaatcgg aaatccagga atcccgcggt ttgatggacg
    29641 ggttgcgggg cgaccgcgag caagtgaacg cgcagttggc ggagggaacg gcgcggaagc
    29701 aggctcagga ggaggaactg gcggcgcggg aagagggctt gcgcgagcag cgccggcact
    29761 tgatcggctt gcaggagcag cgcggggcgc tggaagttga actggcgcag aagaacatgg
    29821 cggcccagaa cgtgcgcgag cagattcagc agaagtatca cgtcaatctc gatacggtgc
    29881 gcagcgagtg cattacgatt acctatgccg acgaagggcc ggcccgggtg catgtgctga
    29941 cgccggagga gatggccgcg gccggggcgg cgaccgattg ggcggcggtg gcgcagcagg
    30001 tggaggcgct gcaggcgcgc ctggacgaga tggggccggt gaatttggtg gccatcgaag
    30061 agtacgagga aaccgaggag cgctaccagt tcctgagcgg gcagcacgag gacctggtgc
    30121 aggccaaggc ggaattgatg gcgctgatca atcgcatcaa cgcgcaaacg cgcgaaatgt
    30181 tccgcgaaac tttcgaaaag attcgcgaga gtttccgcac cctgttcgtc gaggtcttcg
    30241 acgggggcaa ggccgacttg ctgctgatgg acgagggcga cctgctggaa agcggcattg
    30301 agattgtcgc gcgcccgccg ggcaagcagt tgcagaccat ttcactccta tcgggcggcg
    30361 aacagacgat gacggcggtg gcgctgttgt tttcgattta ccaggtcaag cccagcccct
    30421 tctgcgtgct ggatgagttg gacgcgccgc tggatgaggc caacatcaac cgctttatcc
    30481 gggtgctgca gcgttttctg gcgcattccc agttcatcgt catcacccac aataagcgca
    30541 caatcgggat ggccgacgtg ctctatggca tcaccatgga ggagcacggg gtgagcaaaa
    30601 tcgtcagcgt caaattccac aagacccgcg aagaggcggc cgggccgcag gcggcctgga
    30661 tggaaacgcc ggcggtctct ccgccgtccg ccgtggcgcc gggtgagtct cccaagcccg
    30721 acgaggccca cgaactgatg ctggccaaat aggcggttcc gctgaaagtt cgatggtggc
    30781 ggaggaaccg ccggtgatgg ccggagatcg cggtgccgga ttgcattcgg cggccgggag
    30841 gttggcaact cccgcgcttg gctccgggcg caaccgcgtt tatcccgaaa agcggcggcg
    30901 ggtgtgacgc tgggggccgg gttgggccgg gtgtcggcgg cggattcgag gctccgaact
    30961 cagccgcggg cctgttccca ctgttcgcgg aggagtttgg ctgcggcggc gagggctttc
    31021 gcgcggtcgc ctttgcagcc gcattcaaag ctgacgaagc cttgatagcc gatgattttc
    31081 agccccttga aaccatcaat gtaattgtcg ccttcgtctt cgccgggcac cttgcgggtt
    31141 ttgcggctgg cgatgtggac atgctgaagg tatttgcccg cggcgataaa ggccgccagg
    31201 tcgctggttt cctcccaggt catgtgccag aaatcgccca tgagcgccac gccgggatgg
    31261 tttacgtcgc ggcagatcgc cgcgccatcg gcgacttgcc gcaggaagtg gcattccctg
    31321 cgattgaggg gttcgagcag cacgcgggtg ccggccttgt gcgcgtgctc gcccaattcg
    31381 accaggagtt tgaccaggag ttcgcgggct tcctggtgtc ccagcttggt ctggttatga
    31441 aaagcgggca ccatgatcat gcccaccgaa ccaagcgctc cggcggcggt aaggatttcc
    31501 ttcatcgagc taagggcctt ctggcgaact tcctcctgct ccgagatcaa gacgccatcg
    31561 aacccggcgc aaacggcgct gagcttgatt tttcgccctt gcagcgcttt ttgcagttcc
    31621 tccactcgct tgcccaggtc gcgcccgccc acttccagtc cctcgaagcc gttggcttcc
    31681 agaaaatcga gcttttccgt gagggagcct cccgccgaga ccccttcctg gcaggagagg
    31741 cgcaaccgcg ctttctgcgg cggatcgggc agggtgaagg cttgggcggt cggcgccagg
    31801 acgccggcgg ctccggtggc cacggatatt cccaggaatt cacggcgatt cagtttcata
    31861 gtaaagcagc gtagcggata aggtgtcccg gccaaaacca aaaaaggcgg aatctacttc
    31921 agggcggagg tgcgcatttg cagccaggcg acgtcgggat tgtttggcga cgggacgtta
    31981 agcggcaggt cattgttata ccggacgggc ggcattgtgc gcgggtcttt ccatttatga
    32041 atctccgaat ggccatccgc aaaggcgatg ccgcacgcgt tgccatggaa gctggcgggg
    32101 taatcaatga ttttggcgtt ggcgttggtc caggcgcatt cgacggccaa cccggcgtcg
    32161 ttgatgctgt cggggtgctc atccatgacc acccacagca tgtccggcga cggacgaata
    32221 atgtcagtca gtttgccata cttgcaccag ggctccgaga gttggggctg gctgttgtct
    32281 ccccccagcc agggagcgat gaccgagcgc aaggggggca agcgcatcgt ccctaccgcc
    32341 tggctcatgg ccacactgcg aacgcgatta aacgtatgcc cgcccatgct gacggtgctg
    32401 cgatcggccg gacatttgta aatgcctggt gatttggtgt aggggcccag cttgccgtat
    32461 ttggggtcaa tcaggaaggc cacggtataa ttggcgatgt tggcaccatc gaaatccatc
    32521 cggcctccgg accaattgcc attgtcggtg ttgtcctcat tcggcggaag ccactccgcg
    32581 tgatccccgg cgtagagttg ccaggcgatc atgatctggc gcgagttatt catgcaccag
    32641 atgccttggg cgcgcatttt ggcgtgaccg agcgctggca gcagcagggc cgccagaatc
    32701 gcgatgatgg caatgacgac cagcaactca atcagggtaa accctctccg ccgctgccgc
    32761 tggaacggct tcgaagtttt gtctgccgcc gtcatgtttg ttttcacata ccaggttcag
    32821 gctcaggcac tctggggcca cttcgctagc tgtgccgtca gcataccgca ccggcgcggt
    32881 cttcgtcaac cactgaatcg cctattgaaa caaggcgcga tagaattgat accggcgatc
    32941 gccggtggaa agggtcaccc gggccggggc gcggttggtc gagacggcgg tccagtttac
    33001 caggttggtc gaggcttgga tgatgtagtt catgttggtt gcgccgctga gggagaattg
    33061 aacctggccc cggcgggatg gggcgctcaa cacgggcacg tcgtcgtagg gctgtgtttg
    33121 gaccgcgcgg tagaaacagt gttgaggccc cccggggact gccacttgcg tcggtgcgat
    33181 ataggtgccg atgggagtcc agcgagttag atcggacgag gattcaatgg tgtacgcagc
    33241 gccggtgctg ccgttgagcc aaaactgaaa ctggctgtaa ctaaagtcgc tcagggccgg
    33301 cacgacattg atgggaacga tctggatcgc gttaagcggc ccgcgcggat ggccgtccga
    33361 ggcgtattcc cctgtggccg tgaccgtaaa agtcgtgccg cggacatcgc ggaaacggca
    33421 ataattgccg gccgctgccg cggctcctcc ggtgggggag tctgcttcca gaaagctgcc
    33481 ggcaaaatca gtgtcgcttg catcgaggat gtacttggtg ccgctgccac gggtcgcggt
    33541 aagggtgtat ttgccgacgc gcgtcgcgct gccattgtcg ccgtcgccat aaacgatgat
    33601 gtcgtagagc ggaaatttca cgccattaac ggtgacggtc gtggtggagg agtcactggt
    33661 gtccagatag ccttgcatca gtgcaaaatc ggcgggcgaa tttcccatgt gcgagaactc
    33721 atgcgtgccg ccgggggctc cccaggtgat cgtcatgccg gcttcgaggg ggacattggc
    33781 gccattgccg ccggcggtga ggttaatcca gcctctttgg gggacaaccc cggcataacc
    33841 gtaaccgcag accggcacgc tatcgtcgct ggagccggga tgaatgaagt tgatgccgac
    33901 cggcaggagc gcttcggcga tgctgccggg ccaggcatgg cgctgggcgg ccgcttcgtc
    33961 gaggtcattc agaatatcct ctttgaagta atcggaactg ccgttgatgt tccatccgtc
    34021 atagccgtcg ggccattggt acatgtttaa acagcggtat atcggcttgc cggtagcgat
    34081 ggcggtctga ttatagcggc tgatttcggc ataaacctcc tgcatccagc cgggttcata
    34141 atgcgaaaat gggttttcag gatgcccgcc cttccagtag aagtagccat tgcattcggt
    34201 ggcataaagt ggcaggtggt aaaggtcgcg cggaatgccg aggttcaccc aatccttata
    34261 gacgtagaaa cttttgtaga gcccagcccc gcccccggaa aaccgcgccg tgctgtgaat
    34321 gtcttcgcac gtgtagcccc gcgaggatat gtgcagcgcg atgccatccg gcgggcaggg
    34381 gttggggccg gtattggtga tggcggtcag gagctggttg aggtgctgca cccagtttag
    34441 cgggttggcg tcgtagaaat aattggtgcc gagatgagag ttatacccgg cggcgaaagg
    34501 acccgcgaag caagcgggcg ccgccggcag caccttgtca ttgggacgca cggccttgat
    34561 ggcgttatac actttgcgaa agcagcgcgc gtaattggcg ggggagacat aagcgcattt
    34621 tcccaattcc ggctcgtagg gccattcgcc tgagatgttt aactcattgc cgatcgtcca
    34681 aatggagcaa ccgtgggaat tggccacaaa gctggcgcag cggacggcaa acgcatcata
    34741 gctgttggtg ggtggaatgg tgccgttggg aaaatagccg tggttgatgc ggcagattac
    34801 cgtatgtccc tggttgctca ggaaccggta gtccactccc caggtatcgt tggtgttcgc
    34861 gccgaccgac accgtaaccg tgacccaccc gggcccggcg ccattgcgga tgcgcccgag
    34921 atattccgag gcatccgccg gggttgggtc gccaccgtcg aaaatgccgt acaggtaggg
    34981 tgattcaacc gcaggcagcg cgaatgacag taataaaaac ccggtgaata gagccggcag
    35041 ccgaggaatg cgaatcagtt tcatggtctg ccaggattta taactgatgg gggtggcagc
    35101 atcaagagtt ccgcgtaaaa atcaatcaga gacgcatttg ggcgtgatca ctttgcggtc
    35161 gggcggactc gtcaaatgac atctggccgg gagcagagcg gaaagcgaaa gagccagcga
    35221 tacggtgtcc caacgaagca ctgagtttcc agggcttgag cttagcttcg cgctgcaaag
    35281 aattatcgaa agtttgagat actccggata attcctaaaa ctggtttcac ggtttgctgt
    35341 ccagtttgca ggtggctcag ctttcggtcg cctgtagcgg tgcgccctca cattgctggt
    35401 gctggccccg gtttcggtat atcgggcgcg gggcttttgc aagagaacgc agcattctcc
    35461 tcaggaaccg ctgcctgcga acgccgatca gaacccgcca gcgcccggtg gtttgaagag
    35521 aggcgttcat tgatttacac gctttatttt cattgccttt tcctaaaagc atgacttaaa
    35581 tcaaagcccc ggtaacgggt attaaactaa agtttatcgt gttggaaaat tgccggtgga
    35641 agatatggcc aacggcaaaa cttaagtgaa ttagacataa accagaaaga aagaccatgt
    35701 tttgctatca gtgtgaacag acctcaaggg gcaagggttg cagcacgttt tccgtgtgtg
    35761 gcaaggatga aagcagcgcc tgcctccagg acctcctaat ccatgccagc aaaggcatcg
    35821 cccaatatgc ccatcgcgcg cgcgcgctgg gggctcggga tgcttccgtg gaccggttca
    35881 tagtcaaggc cctgttcatc accgtcacca atgtaaattt tgacgcagac gccattgcag
    35941 cggcggtgcg cgaagccgcg caggttcggg ataccgcgaa agctctttac gaagcggcgg
    36001 cccgcaaggc tggccggact cccgagacgc ttgacggtcc cgccacctgg gcgccggcgc
    36061 cgacgctggt cggtctggtg gagcaggggc gaactgtctc aatcctgtcc cgcacgaatg
    36121 aatacggacc ggacctcacc ggcttgcagg agttgctggt ttatggtctg aagggagccg
    36181 ccgcgtatgc ggatcatgcg cagattctcg ggcaggaatc ggaaatcgtg tatgcgttct
    36241 tcgcggaggc gctgtgtcgg ttggcggagg agccccccac ggccgaccag ttgctggcac
    36301 tgtgcctcaa gtgcggcgag gtcaacctca aggtgatgga gattctggac acggctcaca
    36361 ccagcaccta cggtcatccg gtgcccacgc ctgtgcgggt tgaaccgctc aagggcaagg
    36421 ctatccttgt ctccgggcat gacttgaaag atctggaact tttgctccag caaacggagg
    36481 ggaaggggat caatgtttat acgcacggcg aaatgttgcc ggcgcacggt tatccgaagc
    36541 tcaagaagta taagcacctg gcgggcaatt tcggcggagc ctggcaggat caacgtgatg
    36601 agttccgtgc gtttcccggc gcgatcctga tgacgacgaa ctgcctgcaa gagccgcagg
    36661 ccggatataa agagcgtatc ttcacgacgg gccacgtcgg ctggccgggc atcccgcacg
    36721 ttaccaacgg caatttcggc ccggtgattg aagccgcgct ggccgctccg ggcttcccgg
    36781 aagatggtcc ggacaaaacg atcctggttg ggtttggcca caacgcagta atgagcattg
    36841 cggacaaggt gattgagggg gtgaagtcgg gcgccatcaa acatttcttt ttggtgggtg
    36901 gatgcgacgg cgcgcggtcg gggcgtgatt actacacgga gttcaccgaa tcgctgccca
    36961 aggattgcgt cattctcaca ttggcctgcg gcaagtatcg cttcaacaag ctcgatttcg
    37021 ggaccatcgg cggcatcccg cgcttgctgg acatcggcca gtgcaatgat gcctacagcg
    37081 ccatacagat cgctgtggcc ttgtccaagg cgttcaactg cggggttaac gatctgccgc
    37141 tttccctggt gctttcgtgg tatgaacaaa aagcggtagc catcctcctg acgctgctct
    37201 acctgggcat tcgcaatatc cgcctggggc cgaccttgcc cgcgtttatt acgccggagg
    37261 tcttgaaggt gctggtggag aaattcaacc tgatgccgat caaggatgcc aagactgatc
    37321 ttgctgacat cctgaaaaag gcggcgtaaa atagtcgtgg cggcattagc gcgaggttgg
    37381 tcccgcgctg gggcgtggag cagatccctc cggcgcgggg ctttgacttg ttttgccggt
    37441 gcggatgacc ggaactttct tctctcggca gcagtcgctg tgcgagcggt agatattcca
    37501 acgaattgtt gcgtttcggc cctggagacg ggcaagcgtc ccgcgacact ttggaggcgt
    37561 attcgagtat cgctggattg tgatttttac agtgccggat agggtcagat agaggcggac
    37621 aggcattgcc tgtgctgaaa tcgagtccgg agggcaaata cctgggcata ttctgacaca
    37681 tttcggcaaa gggtcgcttg agatagccaa ttgtagtctt gccgaaaaat gttggcttgc
    37741 cttcgtttcc aatgcgctgg agtttccggt tcccttcctc acttccatcc tcaattgctg
    37801 gatgcaggcg taatccggcc atagaggggg ggcggttcac ttccggtgcg cgagtttaac
    37861 tatcgccctt ttgcgcggtc ttaatatatt gctggcgtga tagatagcga acggaagttg
    37921 gcggcgtatc tgccggaatt gcgggcggcg acctgggtgg ccgtggacac ggaggcagat
    37981 agcctgcacg cctacccgga aaaagtttgc ctgatccaga tcagcaccgc ctccggcgac
    38041 cggcttattg actcgctggc acgcctcaac ctgaagccgc tgctggaagt cctggccggg
    38101 cacgagttga tcctgcacgg agccgattac gatctacgcc tgctgcgcag gcaccacaag
    38161 tttgtgccgc gcgctatctt tgataccatg ctggccgccc ggctgctggg gcagcggcag
    38221 tttggccttt gccatctggt gaagcagtat ctgggggtta cgctggagaa aggggcgcaa
    38281 aaagccaatt gggccgttcg accgctaacg gagcgaatgg agcgctatgc ccggaacgac
    38341 actcattatc tcaagccgct ggccgatagc ctccgcacag agttgggaat tcgggggcga
    38401 ctggcctggc accaggagtc ctgcgtgcgg ctgatccagg acacggcggc agagcttccg
    38461 cctgatccgg attcgctctg gcgcgtcaaa ggcagccatg cgctgaatcg cccagccctg
    38521 gcggtcttgc gggagttgtg gcactggcgc gaacgggaag cccgggctgc caaccggcct
    38581 ccgtttttcg tgatgagcca tgaagcgatg gttcaactcg ctgccacagt taccgtagcg
    38641 acaccgtcgg tcgaaccgct tctgccagcg cgcctttccg aacgccgccg ggccagcctg
    38701 aagcaggctc tggagcaagg actgcgcctg cctcccgagc agcatcctca ccatcctcgc
    38761 cgcgtcaacc atcgggtcag tgaaggcgaa aagcggcggt ttagcgagat tcaaaagcgc
    38821 cgcgataccc gcgccaccgc ccttggcatt gatcccacgc tgattgccag ccgcgctttg
    38881 cttgcgcgcc tggctcgtga ctgggaacga cacgtccccg aattgatgtc ctggcagttg
    38941 gagttgctaa aggcatagag aggcaaggtg ctatcggtgg cctctttact tctggaacaa
    39001 ccgattcgcg ggtgtgtgga gtccgccccg ggatgcactg gctatctcac cacctcgaac
    39061 gcaaatcctt tgagatactc cgtttcggga atcatcggaa tgatggggtg atcaggggat
    39121 tgcgaataga cggcgatgcg acggaggatg cggcgggcgt caaacgcggc agagagaacg
    39181 gtatcctgaa acaagctact gttcacgtgg tgcgagcagc agaaggtagc cagtgtgccg
    39241 ccagttttta tgagcttgag ggcgcggaga tggatctcct tatacccccg aagcgcgtcc
    39301 ggtacggaag cccggttgcg cgtgaaagaa ggcgggtcga gcactattaa atcccagcgg
    39361 ggaatcacct gttcatgggg agaccgggtc gtttgggcct tgagccagtc gaaggcgttg
    39421 actgtttcga aggagcacag ttccgccaac ccattggccg ccgcatggcg cgtcgcggca
    39481 gccacggcct cggcgctttg ctcgagcaga tgcacatgcg ccgcgccggc gcgcgcagca
    39541 tgcaggccga aaccgcccag gaaactgaag caatcaagca cttgcgcgcc tttcgcccac
    39601 ctggccacgc ggggataatt gtcttgctgg tcgagataca gccccgtttt atgacccgcc
    39661 agcacgtctg cctcgaattg cagaccattg agtttgatgg gcaccggtcc cgcgagcaat
    39721 cctgccagaa ccccgtgagc ctccggcaac ccttcgaact tgcgcgcgat ggcgtccccc
    39781 cgttccacga ttgcgcgggg ggaaaagatt gctgtcaagg cagctacgat taatggcttg
    39841 cgctgctcca ttccgagtga ggaaatctgg ataaccagca cgtcctcata tttgtctatc
    39901 accaggccgc tcaggaagtc gctctcggcg ttgacgacgc gaaaggaagt ggcgcccggt
    39961 aaatgcttct ggcgcacggc cagtgctgcg cggattcgtt tctcgaagaa cgcctggttg
    40021 acttcgatcc gatcggaggc cagcattcgg acggcgattt tcgattgagg atggtaaaag
    40081 cccgttccca gaaaacgctg ccgatggtcc ttgacctgga caagggcgcc ctccggagcg
    40141 gaccgtgtaa gccggatcac ggagctttga tagatccagg gatggccggc cacgatgcgg
    40201 tcggcctcgc ctggtttgag tattaccgtt ggcaacgatt gcattacccc actcttcctg
    40261 ctacaacccc cgccatgcaa gccgttatta attatcttaa gcagcatcag gcgcgcgcca
    40321 tcgaggaact gtgcgcgtat gtgcgtttcc cgagcgtgtc cgcccaaccg cagcaccggg
    40381 ccgatttgcg ggcctgcgcg gaatgggtgg tgaagcattg ccgggctatc ggcttgcaag
    40441 cccgcctttg ctccacagcg ggccatcccg tggtagtcgc caaaactcca cgagccggga
    40501 agcccggcgc acgtcgtccg catttcgtgg tatatggaca ttatgacgtg caaccgcctg
    40561 agccgttgga cctttggacg tcacccccct tcgaaccgcg cgcagctcgg ggcgcgattt
    40621 atggccgcgg cgcgagcgat aacaaaggcc agaacctggc gcatctcaag gctgtggaag
    40681 cctatttgaa aacgggcacc cctttgcctt gcgatctcac cttcgtcatc gagggcgagg
    40741 aagaaatcgg cagtagcaac ctcactcctt ttctacagca ataccgggcg gagttggcct
    40801 gcgatgcaat tgtcatctcc gatacgggca tgccttcgcc gaaacacccc gcactcacct
    40861 atgcgctgcg gggcatcgcc gggttcgaaa ttatcctgcg tggtccggcg caggatttgc
    40921 actcaggcat atttggcggc acggtggata atccggcgat ggcgctctgc caactgctgg
    40981 gcaaactgcg cgacgccaaa ggccgcgtag ccatcccggg cttttatgac ggcgtccggc
    41041 ccttgtccgc ctacgagcgc aagcagttcg cgcgggtgcc cttcaacaca cggaattacc
    41101 agcggttgct gggcgtgccg aaactctttg gcgaagctgg gtttaccccg gtcgaacagc
    41161 gctccgcccg gccgaccttc gagatcaacg gcctgaccag cggctaccag ggcgaaggca
    41221 gcaaaaccat cgtgcccgcc tgggcgcgcg ccaaagccac catccggttg gtgccagatc
    41281 aggatccagt ccaggtcatt cggtgcatcc gccgccactt ggctaagctc tgcccgccgt
    41341 cagtgcgctt ggaacttaag gagcgttcct acgcggcacc ctatctggta tctcccaccg
    41401 gcccccaggc gcaagccgcc ttacgcgccc tcaagcgagc ctttgcctgc gaaccggtgc
    41461 tcgttcgcga gggcggctca attcctatcg ttaacgactt caagcggatc ctacgcgctg
    41521 acgcgctctt gctcggcttg tcccttcccg acgacaacgc ccactcaccc aatgagaagt
    41581 tcgatttggg ctgtttcgcc aaggggcaac tcatgagtgc atatctctgg caggaactga
    41641 gccgtactta agccgaagcc gaatcgcgcc ggcgaaccgt tcccattttg acgtcctgcg
    41701 ccctccgcgt tggcccggcg gccggtgcga ggagcatctt tagcttgccg gttataccat
    41761 ttcctgttag tagttgcatt gtattgagag gcatggcaag a
//
LOCUS       DAOPQG010000016        41577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1249_length_41577_cov_16.288690, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000016 DAOPQG010000000
VERSION     DAOPQG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 41577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41577
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_41577_cov_16.288690"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PKI07_03085"
     CDS             <1..554
                     /locus_tag="PKI07_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50065.1"
                     /translation="AEDGVRLRLNVGEAHEDIMVFGQPPCSAGRMKPRRAYYLGLLGP
                     SQNGQSDITDLYAARFGRPAPGQKVFIAVVQTRNGWKGQSRVHNAIVPPPPMLKSQQA
                     AETARVKKSKPAPAPASTTARKTVSSPSFRVMYKGSTSEARQEHMENKHEPPLSLRCT
                     PVVHGLMGALGWLGRVGRVWARA"
     gene            1053..1415
                     /locus_tag="PKI07_03090"
     CDS             1053..1415
                     /locus_tag="PKI07_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50066.1"
                     /translation="MKTLFFLPAGGLRVAAGVCLAVWLTGCATQRVDWPARIGQYTYD
                     QAVAEMGPPEKQAQLDDGRRVAEWLLNRGSTYVQATPHLYGPYYGGFTTVQTAPSRFM
                     RLTFDTAGRLADWKKVYR"
     gene            1691..1906
                     /locus_tag="PKI07_03095"
     CDS             1691..1906
                     /locus_tag="PKI07_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50067.1"
                     /translation="MAECLAELHRARQPFRVFDSGLAVSIFRDKSTRTRFSFASAVNA
                     LGLGLAELDEEKPQLAHGETVRETANP"
     gene            1951..2562
                     /locus_tag="PKI07_03100"
     CDS             1951..2562
                     /locus_tag="PKI07_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50068.1"
                     /translation="MASEAWQCPTLLLNGFGEHRIEGIGDKHVPWGHNVRDTDAVAAI
                     DDEDCLRILRLFNEPAGREHLVARGVGEGEVEQLGWLGISSIANLLAAIKTAKYFELG
                     EADLLLTVFTDSTDLYASRLAELREQRGGLYVERRRGRLCRPAGAPGPGRFQGADVPG
                     AEGHSQSQILHVGRAAGENRAGTQRAMGPGVLARPFRGRSGVF"
     gene            2696..4063
                     /locus_tag="PKI07_03105"
     CDS             2696..4063
                     /locus_tag="PKI07_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOC50069.1"
                     /translation="MDVLLLSRIQFALTTSFHYLFPPLSIGLGLLLVIIEAVWLKTRN
                     PLYHQMARFWTRVFALTFAIGVATGLVLEFEFGTNWATYSRFVGDVFGSPLAAEGIFA
                     FFLESGFLAVLLFGWDRVSPRLHFFSTCMVCLGAHFSAVWIVVANSWMQTPAGFHIVG
                     EGLRARAEITDFWQMVFNPSAVDRLSHVLCGAWQAGAFLVVSVSAWYLLKKRHTEFAR
                     ASLRIGLLTGLAASLLQLATGHSSAAGVARNQPAKLAAFEGLYDTMPRAPLTLFGWVD
                     ERAEVVRGGVAIPGLLSILSYGDIHHPVQGLREFKPEDRPPVNLSFQFYHIMVGVGVG
                     MIALAGLGCWYFWRGSLPGQRWLLWLLTLSVLGPLLANQTGWFAAEVGRQPWLVYGLL
                     RTSDGLSAVVRANTVLTSILLFSAVYLLLFAVFVYLLHSKIQHGPDAADLTPAGKLAR
                     PPRAN"
     gene            4117..5148
                     /gene="cydB"
                     /locus_tag="PKI07_03110"
     CDS             4117..5148
                     /gene="cydB"
                     /locus_tag="PKI07_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOC50070.1"
                     /translation="MSGAGLDLNVVWFALVGVLFTGYAVLDGFDLGVGALHLFTRTDE
                     ERRVMINTIGPVWDGNEVWLVTGGGALFAAFPNVYATVFSGFYLALVLLLLALIFRAV
                     AIEFRSKQPMRWWRQMWDFGFSAGSLAACLLLGVAIGNIAWGIPIDARGEFAGTFWGL
                     LHPYALLLGVTAVAVFMTHGALYALMKTEGALHDKLRRWSKRCFILLAVCSVAATLAT
                     LFCVPHLAARARANPWLFGLVLVNLLAIANIRREVQRGRDGRAFLSSSIGIMALMGLF
                     ALGMFPNLLWSHPDPAHNLTIYNAASSPRTLGIMLTIAAIGMPLVLAYTAFIYWIFRG
                     KVRLDRASY"
     gene            complement(5218..6195)
                     /locus_tag="PKI07_03115"
     CDS             complement(5218..6195)
                     /locus_tag="PKI07_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50071.1"
                     /translation="MIRDILQSDVAFARRLLNENPAEAEIVQALIRRGLDPDKAAQLV
                     SDLRDGRPVSYDANASLEFTPRRRSRSRSNAAGTANGPSGSSTESAPRRKPSAPPAAR
                     PGRRRFPWKLFIALLALALALQAIVIAILVRQQKDPFSPPRSETAAATPRDNSPRPVP
                     PGEAGGAAPGNTAAPVLLELQPDGLYLGGERVSRENLLPAVARQLGAPSRTNRVAQTG
                     AIIYVFDRHGLLLYAQPDGGPTSIVLDCEGTGGNAGATTPFAGRLKVENTEISPDTDS
                     LSLAAIQKLPLTRRGRTSNILTGHYGKFELIFGYVRSNQRLSLVEINLK"
     gene            complement(6292..7680)
                     /locus_tag="PKI07_03120"
     CDS             complement(6292..7680)
                     /locus_tag="PKI07_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOC50072.1"
                     /translation="MRGNILNLLLTGMAAILAALPASSPAAQLPNLIIILADDLGYGD
                     LGCYGHPSIRTPNLDRMAAEGMRFTDFYVGACVCTPSRAALLTGRLPIRSGMSGDLQH
                     RVQSRKSPGGLPPEEITIAHALKEKGYATFCVGKWHLGHYQQNLPLCHGFDAFYGLRW
                     SNDMEPTRGIPKGASSSLNPNPEWWSCTLMRNNQPLEQPTDLSTLTRRYTEKAVRFIQ
                     QNREKPFFLYFAHTYPHIPLFASKAFQKTSPRGLYGDVVEELDWSVGRVLETLRQQKL
                     DQQTFVFFTSDNGPWLIKELVGGCAGHLQGGKGSTWEGGMREPAIAWWPGRIKAGTVN
                     RELACSMDLFNTCLALAGVPLPADRIIDGVDMTPMLLGQGPSQRNHVIYYNGDEVYAL
                     RKGAYKAHFTTYSGYGEDTPERHDPPLLFHLPNDPGERFNVAADHPEIIADLRQELEK
                     HRAGLVPGKRQY"
     gene            complement(7774..9258)
                     /locus_tag="PKI07_03125"
     CDS             complement(7774..9258)
                     /locus_tag="PKI07_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOC50073.1"
                     /translation="MNSHHRALRVAGACLALLILLTGSFSSRGADRPPNIVFILADDL
                     GYTDVGCYGSKYYETPNIDRMAASGIRFTSGYSCGPNCAPTRAALMSGQYGPRTGVYT
                     VGSIDRFNWRSRPLRPVDNVIRLPLDKITLAQSLKRAGYATGMFGKWHLGDGERYHPR
                     QRGFDEAIVSAGVHFEFTTQPPVEYPKGAYLADFLTDRAVDFIRRHKEQPFFLYLPHY
                     AVHQPLQAKDSLVAKFQAKPTVGGHNNPTYAAMIASVDESVGRVLALLDELRLAPNTL
                     VIFSSDNGGVGGYVREGIKPTGDVTDNAPLRGGKGMLYEGGIRVPYIFRWPGRIPAGT
                     VCDQPINSVDLYPTLLAVAGASPPPDYPLDGVNYLPALTSGGKTGLRREAIFWHFPGY
                     LGAGAGAWRTTPAGAIRSGDWKLQEYFEDGRLELYNLRTDLGQTNNLAAKLPDTAREL
                     QARLAAWRKSIHAPMPTRNSGSPPPAPGKKGKKSRPLKNAGPDK"
     gene            9625..12477
                     /locus_tag="PKI07_03130"
     CDS             9625..12477
                     /locus_tag="PKI07_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HOC50074.1"
                     /translation="MWVAGAAAAGPGLTPAQLRCEYRTNPLGIDELRPRLSWVVASGE
                     RGQRQTAYQVLVASDETRLRKGRGDLWDSGKVAGGETTGVVYSGKPLGSRQLCFWKVK
                     VWDKDGRASDWSQPAQWSMGLLKDKDWQAQYISFRDTSPVHKFKEPLFLPPARQYRKE
                     FAAARPVRRATLYATALGIYELHLNGQRVGDEWFAPGWCDYHQRAYYNTYDVTALVKR
                     GANALGAWVADGWYSGYIGFGLLTGIGTEAIGRYTYGKTPALQAQLEIEYADGSRQVV
                     ATDASWKVTGAGPIQEGDFLMGESYDARQEMHGWAEAGFDDSRWEAAIRAEANGSAPA
                     KFYEYRNPTGGGGPKIEGREVDLAFKPPPKREAFPGVPVRAVEEIRPTGITSPTNGVY
                     IFNLGQNFAGVARLKVKGPAGTRVRLRYGEMLHPDGRLMTENLRKARATDYYTLRGDP
                     LGETYVPRFTFHGFQYVELTGYPGEPGLEAVTGIVLQSDTPLASAFECSDPVVNRLFK
                     NIVWTQRANFLDLPTDCPQRDERFGWTGDAQIYVAAAAYNADVAAFYSKWLRELMEAQ
                     RPSGVFPGYAPYPFQHGWDFGTAWCDAGVICPWTIWQAYGDTRIIERCWEPMTRFIAW
                     RKAASREFLGVAHGNEWGDWLSVNEKTPIDYIDTAYFAYSTKLMAEMAQAIGKTAAAA
                     DYSALLGKIKAAFNRKYVQADGALSVDTQTAYALALYMDLLPESLRAAAGARLAKKIE
                     ANEGRMSTGFLGTRPLLPALSATGQSDLAARLLQSRKYPSWGYEVEQGATTIWERWNS
                     YTKDKGFGGAQNAEMNSFAHYSFGAVCEWMFSQLAGIQSDGPGYRRIIIRPTPPSPGS
                     NPDQAPIDWVRAHYDSIHGRIASAWKVEGERFRLELALPANTTATVYVPAREAASVTE
                     SGHLLEQSPGVRFLRMEGNQAVLAVGSGSYQFASRL"
     gene            complement(12730..13569)
                     /gene="tsf"
                     /locus_tag="PKI07_03135"
     CDS             complement(12730..13569)
                     /gene="tsf"
                     /locus_tag="PKI07_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOC50075.1"
                     /translation="MAEITAALVGKLRGMTGAGMMECKKALTEAKGDLDTAVDLLRKA
                     GTVTAGKIAAREAREGVIARYVAPDGRVGVLVEVNCQTDFVARNEEFRSFADEVARRL
                     AADPQASLEAEREGLVAKIRENIKLARHARMEVAGNGMIAAYIHTGAKVGVLAEVGAG
                     REETLAKDDFKQLVKDITLQIAAGNPLTVSREQVPADVLAKEREIAAEQFKNKPPQAV
                     AKIVEGKLEKFYQGYCLVDQGFVKQNSEISVKEHVASVAKQLGDEVTIRRFVRFQIGE
                     AAA"
     gene            complement(13672..14499)
                     /gene="rpsB"
                     /locus_tag="PKI07_03140"
     CDS             complement(13672..14499)
                     /gene="rpsB"
                     /locus_tag="PKI07_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOC50076.1"
                     /translation="MIAIGVKELLDAGVHFGHQTKRWNPKMKPFIFDARNGIHIIDLG
                     KTINQLEAACNFLGATVRKGGKVLFVGTKKQAQQAIKQTAKETGQFYVTERWLGGTLT
                     NFATIKRSIGRLKEIEKMEADGSINNFVKQEQSVIRREAARLKKFFEGIQTMDKLPGA
                     LFVVDIKREHNAVAEARRLKIPVVAIVDTNCDPDLTDLPIAGNDDAIRAVRLILAQVG
                     QTVMQAQAEYETKHARRKADEEAAATMPEAQPEPAPAVPVEAAPSVSATDTAAPAAA"
     gene            complement(14741..15397)
                     /gene="aat"
                     /locus_tag="PKI07_03145"
     CDS             complement(14741..15397)
                     /gene="aat"
                     /locus_tag="PKI07_03145"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOC50077.1"
                     /translation="MRFTLLDHQLHFPNPRGADAEGLVAVGGDLSVPRLLLAYRSGIF
                     PWTVDPITWWSPDPRAILTWESFHVPRSLGRILRQGTYQITRDQAFTRVMKACAAPAP
                     GRRSTWITPEFIAAYAQLHAQGHAHSLECWQGRRLVGGIYGVTIGGLFAGESMFHRVS
                     NASKVALAHLVEHLRQRGFELFDLQMLTPVTARLGGITIPRAEYLQRLARAVSQPVHF
                     "
     gene            complement(15480..15944)
                     /locus_tag="PKI07_03150"
     CDS             complement(15480..15944)
                     /locus_tag="PKI07_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50078.1"
                     /translation="MKLVPTPGARLLFHLDRQEAFLLPQILQLYPRIPPAHQALSRTG
                     RIPHPEANQQLLNEALAEQRAENRQQVLALLADRQRFAPTKTGGRLSLAPAEVEWLLQ
                     VLNDIRIGSWIQLNEPEATPPKLDDVSVRDLLFMEIAGFFELQLLAALHKEE"
     gene            complement(15941..16249)
                     /locus_tag="PKI07_03155"
     CDS             complement(15941..16249)
                     /locus_tag="PKI07_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOC50079.1"
                     /translation="MAGIATPEIQPGLVQTTEDRPKSSLDPGYLVICWNDSVNYMDYV
                     THVFQKVFGWARAKAEFHMRQVHEQGKSVLARESLEKAEHYVHQLQKYSLHATMERDE
                     "
     gene            complement(16571..17872)
                     /locus_tag="PKI07_03160"
     CDS             complement(16571..17872)
                     /locus_tag="PKI07_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOC50080.1"
                     /translation="MNNTSKNLRTLALHAGQTPDPVTGARAVPLYQTTSFIFKDSDHA
                     ANLFALKEFGNIYTRIMNPTTDVFEQRVAALEGGTGALAVASGQTATTLTLLNLSQLG
                     DEIVSGSNLYGGTYQLFHYTLPKLGRTVKFVDSQKPEAFRQAITAKTRALYVETIGNP
                     RLDVPDFAALAQIAHDAGLPLVVDNTVGVGLVRPLDHGADILVASATKYIGGHGTSIG
                     GVIVDSGKFQWNNGKFPEFTEPDPSYHGLKIWEAFSNVPGMGNVAFILKARVQLLRDL
                     GTCLSPFNAFLFLQGLETLPLRQQQHSANALALARWLQQHPLVSWVIYPGLPDNPNHP
                     IAARYLKHGFGGILGFGIKGGLAAGRKFINAVKLLSHLANIGDAKSLVIHPASTTHQQ
                     LTPAEQAAAGVTPDYVRVSVGLEDLEDIQADIDQALSASQQ"
     gene            complement(17999..18247)
                     /locus_tag="PKI07_03165"
     CDS             complement(17999..18247)
                     /locus_tag="PKI07_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50081.1"
                     /translation="MKRQVLVILSNRLNRLQKPRFIEITCDEQGNILRQSTLRRPPRE
                     ARFDEVWENDDGKTDFASCHSFKRQYGHALQKPKNRAR"
     gene            complement(18254..20461)
                     /locus_tag="PKI07_03170"
     CDS             complement(18254..20461)
                     /locus_tag="PKI07_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOC50082.1"
                     /translation="MRRSGLSVAIGLAVMALAMAGALPGTTRAEVRLPHVFSSHMVLQ
                     QDQPVLIWGWAQPGETVRVALANLHQQVTANDRGEWKAKLPALKAGGPHVLLVSGSSS
                     VRCEDVMIGEVWLCSGQSNMEMGVGVSKDAKAEIAAAHHPGIRLLKVGKSWKPEPQND
                     IEGAWKVCSPQTIAEGGWGGFSACAYYFGRELHQKLGVTIGLIDSSWGGTRIESWTPP
                     EGFAAVPALQTQYEQVQLGDPRTQLHQQRLEQFLQVVGRWMASARQALTDRQLVPAMP
                     VYPEELLPPRDLQHPTALYNGQIHPLHPYPLRGAIWYQGESNATEGKLYTERMKALIR
                     GWRQVWGQGDFPFYFVQIAPFTYSFDPETLGEFWEAQTAAQAVPFTGMAVINDISDLK
                     DIHPTNKQEVGRRLALWALARTYGQEAVAYSGPTFKSLALEGDALRVTFDHVGGGLVS
                     RDGQPLNWFEVIDADQGGFVKADARIDGASVVLRAPEVKHPVAMRFAWHMLAEPNLMN
                     AAGLPAGAFRAGQAPKRDWLELKVPEAKDYQLVYDLDLNKLGRDFVYEVDNSSKIQRP
                     FDRVAYFLELQPADRPTQYLYVSMDAFTDAPGKLGVPRGRTGAHFQQNIANLNVYSNV
                     KGIVTGTGLAGGNLEFWPNNYGPANAANVPHASAQTWDFGDAPSAEVAGYGSMQVHNH
                     EARQTLFALNHWNEGSRADLGIGNQPQGNPDWTFAANAGTYKTKRLRVLVRCK"
     gene            20636..21850
                     /locus_tag="PKI07_03175"
     CDS             20636..21850
                     /locus_tag="PKI07_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOC50083.1"
                     /translation="MAVIKPFAALRPKPELAPRICELPYDVMSSEEARQIAAGNPLSF
                     LHISKPEIDLPPATDPCAPEVYVQGRKNFRRLIAQGALRQDTQPCFYLYRQVMGAHSQ
                     AGLVAAASCADYREGRIKKHELTRPDKEDDRLRHIEALESQTGPVFLVYRATPGLDAL
                     VARQTTAAPDVDFMAPDGVRHTAWVIAEAAAIQFIEAEFARMPGLYIADGHHRCAAAA
                     RVCQARNGAGGSARFLSVLFPHNQMQILPYNRVLKDLNGLSPAQLREKLASVFTFQHP
                     GVATPARKHTLGFYLEGEWHGLHFLPPYTAVADPIERLDVTLLQKQVLAPIFDIHDPR
                     TSERINFVGGIRGTAELEKLVNRGEYACAFSLFPTSIEDLMTIADAGGIMPPKSTWFE
                     PKLRDAMFCHMI"
     gene            21915..22964
                     /locus_tag="PKI07_03180"
     CDS             21915..22964
                     /locus_tag="PKI07_03180"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOC50084.1"
                     /translation="MNTTRSFQRLLQGPAPLLALAPMQDITDLPFLRLIAGYGGADVY
                     FTEYFRVYATSRLNKHILKSITENPTGRPVIPQIIGSDIPALVRTARELQQYPVPAVD
                     LNLGCPAPVVCRKGAGGALLRDPKRVDAILGALREAVRVPFTVKTRIGFDAAEVFDDL
                     LPIFARHALDLVTVHARTVKEMYRTEVHYDFIARAVAGLPCPVLANGNVYSSPKAATV
                     LQLTGARGLMIGRGVIRNPWLFRQIREHQRGETVFIPRGTDVLAYVRALYQAVCSPAT
                     RESAQVQKMKKYLNYLGVGVEPTGQFLHQARRVTTTAALFDVCVKFLDHDRPMPLEPF
                     PLALKAGDLMAGEQR"
     gene            complement(22987..23415)
                     /locus_tag="PKI07_03185"
     CDS             complement(22987..23415)
                     /locus_tag="PKI07_03185"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOC50085.1"
                     /translation="MNLPARIRAWWRGHEKPLHLRHGERGERAAKKQLRRQGLKFLTA
                     NFRSPRGEIDLIFRDRDCLVFIEVKTRSSEEWARPAAAVNRERRGRLTRAALDYLRRL
                     RNPPVKLRFDIVEVLLRDGKVREVRHLPNAFAMERPHRYG"
     gene            23499..24473
                     /locus_tag="PKI07_03190"
     CDS             23499..24473
                     /locus_tag="PKI07_03190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC50086.1"
                     /translation="MTAASAARACPVCGQDDATVWLQKNALRLVRCRRCAMIYANPVP
                     TALASGQYYDQGGADYYLSPAKLESDYAPVRYERELRLFRRHCRGGAVLDVGCSSGAF
                     LFQLGRRFPGDYQGLGTDVSGPALDYAEQRGVAVRRGSFLELDFGGRLFDAVTFWAVL
                     EHLLEPARFLAQAASLLKPDGLCFVLVPNMKSLAARLLGARYRYLYPQHLNYFTKATL
                     MKLVAPCFSVITFHTTHFNPIIVWQDWRGGGRDVSPRERAQLLQRTTAAKQHPLLRPM
                     KALYGLTENALAALTLADNLAVVLRRREAREAESSPQPDWFASTGREG"
     gene            complement(24453..25001)
                     /locus_tag="PKI07_03195"
     CDS             complement(24453..25001)
                     /locus_tag="PKI07_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOC50087.1"
                     /translation="MTACQILKAEHDRIAAVVNALEVIAAGVDNGQLPAPGTIAGAVE
                     FLRGYADQLHHGKEEALFFPRLVERGVPSQGCPIGGLTHEHEKGRALVSALAEWSPAP
                     GQDPRDALPALREILRGLVALYRDHLWKEDEMVFPMADRFLTPADQAELSRQFADLDQ
                     SFGPEAVRRLEQLAEELTLTAG"
     gene            complement(25030..29064)
                     /locus_tag="PKI07_03200"
     CDS             complement(25030..29064)
                     /locus_tag="PKI07_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOC50088.1"
                     /translation="MTAFAGLEDRLVDESFLSPLPPSPDWSSIDVPPKGAEATAPSKG
                     FGPIAPAATQPAGALSGKLVFMNGGHGWTYKSGWVLLRPVLLDMNEDYGNVDQLNMFA
                     TYCFNAGAVVIPCRPLGYQTNEVVLDNDDAAVKFDGTWFNSTATSYFYGTVGDVPYRY
                     ANLDNTETATATYTPTIPVTGYYPVYCWASHGANRGDQLYRIRHTGGESQVRIPHYRV
                     GNGWVYLGEYYFNAGSNAAVGAVIVSNLRNTANGSVVIADAIRFGNGMGDIDRGGGTS
                     GYPREEESCRYWVQAGLGVGMPTEIYDSGANDESDSWSTPPRISAEMNREASGSVHDR
                     IHISFHSNAGGGRGTVALITGSPTTNQAALAQICGKEVNDDLVALGSPPLEYPWYNRT
                     TVTYTGGYSEIDGRLFNYEMPATIIEVAFHDSATDSALLRDPKARAAVGKAAMHAVVK
                     YMNTYGSGPLAFLPEPPVNPRALGWANGNITLQWGIPPSVGGSQSPTNYVIYSSTNGY
                     GFGNPISVGNVTSYTVSGLAPGQAYYFRIAAANAGGESLPSEVVGCRTPATAGAPRVL
                     VVNAFDRFDRTTNPRMNVGARNYRPPGNSGSIERVWPRRINAFDYVVPHGQAISAYGM
                     AFDSCQNEAISASQVLLSDYPIVVWACGNETENSETFSSVEQSRVATFLAGNGSLFVS
                     GADVAFDLDRPSGPTAADRAFLRQQLHATFSSDNSASYTANAAPGGIFAGRASVTVDD
                     GTKGIYWVQTPDVITPYGTGAAVAMNYAAGGAAAVQYDGSAGGGRTVLFGFPFETITS
                     ATRRNQYMADVLNFLSAPPATNMAPIILTPPAGQLVVQGADAVLSVAAAGTAPLAYQW
                     RFNGADLAGATQSSLTRNSAQPAHSGYYQVVVSNAFGTVTSPAALLEIMLPPLQTLFL
                     DDFDSNTAANWVVNRSSPDCRVTFNYNYAPYGIPAAPNSANGSTRGVKFEANVSLGVA
                     AAINISPVGQSFSGDYQLRFDLWMNQNGPFPAGGNGSTQHGTAGIGTAGNRVQWTGAG
                     STADGYWFAADGEGQASDTSTTAINDFGAFTGTTHHSAASGVYAAGTASNARGNGNSY
                     YAATFPGGQTAPAVQAQSGALAVGTIGFAWRDVIISKTGTTVDWSIDGLKIATIPGAS
                     FTSSNIFIGLWDFFDSLSDSTNLSFAMFDNVRVERFVTNIPPYLTAQPQGLTVVQGGQ
                     ATFGVDAGGTAPLAYQWRQNGTNIAGATASSYTRMNLAPSDAGNYSVLVTNAVGTVTS
                     LNAVLTVTPLTPPLLTQPNWLADGRFQLFLTGDPGAYALYTSTNLFDWSFARFVTNTG
                     ATVEIVDDAATNYPQRFYRIAPAP"
     gene            complement(29373..30683)
                     /locus_tag="PKI07_03205"
     CDS             complement(29373..30683)
                     /locus_tag="PKI07_03205"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOC50089.1"
                     /translation="MIVELINTGSELMLGRVLNTHQQWLCRRLTDQGYTVSRQVAVPD
                     TGPDIRQAVGEALARADLVITTGGLGPTSDDLTRDLIAQLLGKALHEDAAVLAHIRHF
                     FECRRREMPARNRVQALVPAGAIVLPNPHGTAPGLAMEVRPNPFRAGKRASWLVMLPG
                     PPVELHPMFAESVLPLLRRVLPPSGEFVCRILRTTGIGESAVQEQLDAPLRRLVEAGL
                     DLGYCARPGRVDVRLAARGSEAEPRVASAETIVRERLGSRIYGVEGEDLETVVVRLLA
                     ERKATLALAESCTGGCLAHRVTNVPGASAVFLAGLVTYSNAAKQKFLEVRADTLARHG
                     AVSEPVAREMADGVRRHARADYALSVTGIAGPGGGTPEKPVGTVFIGLATPTGVAVER
                     RFNPGERETFKQVTAEQALDWLRRTLLAAAVEPARRPPDCAPDV"
     gene            complement(30808..32709)
                     /locus_tag="PKI07_03210"
     CDS             complement(30808..32709)
                     /locus_tag="PKI07_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HOC50090.1"
                     /translation="MMSPSGSLRSTAHRAGLLAAWLFSCGAAPVAAADGAWLAPSTLA
                     ATPDGRTLFVGCVAGRRVLWLDPAARRVTGSTRMPAPVTGLAVAADGQTLFVTCAAPE
                     SQVCIVDIPRGRITARLPAGHSACAPVLSPDGRTLFVCNRFNHNVSVFDLAAKKEIRR
                     VAVRREPVAAILTPDGRRLLVANHLHAGRADVEHAGAVISVIDPARGQVVDELWLPSG
                     SGLLNDLSLSPDGRYAVATHILARFHLPTLQLERGWMNTNAKTLIDLERLEVVNTVLL
                     DAEDRGAANPWGAAWSADGRKLVVALAGTHELSVTDFPALREKLARLTPVFQPTVSGA
                     YPTIGSQAPSDVPNDLSLLAGLRERRPLPDGDLGPRGVRVIGQTAYTANYFSDTLSVF
                     ELDGPQPKTATIALGPRPALAAAQQGELYFNDAGLCFQGWQSCASCHSDDARTDGLNW
                     DLLNDGLGNAKNTKSLLGAFATPPAMSLGVRESAAAAVRAGFTHILFATPPPAVSAAV
                     DAYIQSLRPIPSPHGRPGKLSAAARRGRRVFRRADTACAHCHSGPWFTNLKSYNVGTH
                     GPCDKADDTFDTPSLIELWRTAPYLHDGSARTLRDVLTTANPADRHGKTSHLSADQLN
                     DLCEYLLSL"
     gene            32873..33448
                     /locus_tag="PKI07_03215"
     CDS             32873..33448
                     /locus_tag="PKI07_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC50091.1"
                     /translation="MERTDAELIAAVRKGDAASFEPLVRKYSARLFGMARRYARGESE
                     VEDLVQNIWLKAFANLPSFRGQAPFEHWLMRLAVRVCYDSLRRHQRHRETLFADLTEP
                     EADWLEQFARQPDSAPEDAEAARQLVAHLLAGLSPAARLVITLLELEQRSVKEVAQLT
                     GWTVPVVKIRAFRARAAMRRQLARVAKEKYL"
     gene            33489..33800
                     /locus_tag="PKI07_03220"
     CDS             33489..33800
                     /locus_tag="PKI07_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50092.1"
                     /translation="MNLDELERKLMAAARSAPPNERVPWAFEKRIMARLAGRPGADDR
                     ALWARAWWRAAAACAGVMVLLSAWTWLTPPGGAPANDLSRALEQTLLAAAELEFPGDW
                     N"
     gene            33800..34261
                     /locus_tag="PKI07_03225"
     CDS             33800..34261
                     /locus_tag="PKI07_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50093.1"
                     /translation="MNNWKVILATMVIFGTGVVTGGLLVRHAERGRILRPPRPGAAVR
                     PAPPVSAGGMRIEFLRRLEQELDLTPAQREPINRILKEGQERTKKIMDTVEPQRREEF
                     SRTIEAFRAVLTPAQQQQLNALLKQQQRRAREQRKAAAPPPRPRPGAAPTP"
     gene            complement(34286..36610)
                     /locus_tag="PKI07_03230"
     CDS             complement(34286..36610)
                     /locus_tag="PKI07_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOC50094.1"
                     /translation="MQVEPTVARDAAGEAPAAPRTGTELAIAGMTCANCARHVAEAIQ
                     SVPGVHSATVRLEAKRAAVRWAGGAARDVDAVIRAIAEAGFEARIADAPAAGAAPGRW
                     QLNLWVGILGTAPLMLGEWGLGLGLTRWFQWVSLALASGVQIVAGAPFYRGAWSQLRA
                     GRSNMDTLVALGSTTAFVYSAWALLRGAGGHVYFMEAAAIITVVSVGHWLESRVSARA
                     SSALRQLLNLAPALARRRDPDGAEREVPVAELREGDRVVLRPGDRVPTDGAAEEGSSV
                     LDESMLTGESAPVDKAPGSRLYAGTVNLSGRLLMRVTATGEETALAHIIAAVQRAQAS
                     RANIQRLGDRVSSVFVPLVVGVALAAGLSWGLAPDWARRVHDSLAPLLWAAHPPEGAL
                     AAAFIIAAGVLIVACPCAMGLATPAAIMAGSNAAAQRGILIRDGVALEKAGRVGTVLF
                     DKTGTLTTGRAEVVKVWDEGRLLNPEAAPGGSNAGPAAAASARELAAALAEHSSHPIS
                     QAVAKLAAARAPLAGWQEVRGSGVTATLAVDRGAPVAVRLGALRWLEESGVDLAPGKD
                     FIAEWAAQGATVVGLAVANTLRGLFAVKDALKPGAREVVRQLARQGLQIYLVTGDNAL
                     TAAGIARQTGISAANVFAQVRPEQKAALVQKLQRQGQRVAFVGDGINDAPALEQADLG
                     IAVSRASDIAREAADIILLRSEIEAVPESLGLARATLRTIKQNLFWAFFYNILAVPLA
                     ALGFLSPVLCAAAMGMSDLVVIGNALRLRRWRSR"
     gene            36792..37868
                     /locus_tag="PKI07_03235"
     CDS             36792..37868
                     /locus_tag="PKI07_03235"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC50095.1"
                     /translation="MKPNHLLVALLLVLAGGVMRVHAQPTEAERKDFASVQARANKGD
                     VEAQLLLATYYTQGIGVTASPKKAVQALRQAALQGSAIGQYRLGLKYLDGEGVKADKV
                     EGARWIRRAADQKLAEAQFVLGQCYTWGEGVGRDVVEAANWYRKAADQDLADAQTELG
                     ICYFEGNGVPKDIPEGVKWTRRAAEQGFARAQNRLGLCYSKGTGVPQDYQEAYMWLNL
                     AAAQGDARAEDAKINLAAAERFLSPEQIAESQRQAREFRPRKAGSPAPPPPAPAPTTA
                     PAPPPPAPDRPAPAGFVNVSAPDDACEIFVDGAFVGHTPARVKLAVGTHEVEVKKPGF
                     RPYRRPLQITEGSELTLRAVLEKQ"
     gene            38000..40414
                     /locus_tag="PKI07_03240"
     CDS             38000..40414
                     /locus_tag="PKI07_03240"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50096.1"
                     /translation="MKYRLCFTLLLLLVFTPTPMLAQLAAFTYQGQLLDDGEPANGSY
                     DLRFTLADAPAQGHLIAGPLTNAAVAVRDGLFLVTLDFGGSAFDGSPRWLEIEVRPAG
                     PSAPFIRLSPRQPITATPYATLAASLLPGAAVALDGAGITNLDASALARGTVPLARLR
                     GITATELDAHTWQLATQGTAGLSFTPQFGHANLTNWAKLETNVLAGLAGAGTSLAAGT
                     NWLRAISNAVPGVYTNSGNLWVSGSTLPAANGLYTLAAGAVFELCTNTVVWTNQNHYG
                     AFAPAVRDVDIWSNQPLTITNPSGAAVLSNASFVSSGDVVYYVPGYPAGIFLTNNGVA
                     DGVLHWASNTLVEPGWHVAGWQASGPLQLEGGRYVGDASGLSNMPPVVQAKLNDTLRV
                     SEGGVFRRIFHAPPLGICTWPAFSRQINDEVVRGLADSLYTNHLVELGWRLLQLDGGW
                     QGGTNNSGRDTNGLLTYHPLNFPDWDGTRRYLRERGLTLGLYHEMTKGNEGMRLTNHY
                     VADAAALHSWGADYVKIDEGTRTLAGRFSEFAALRAAGDAIGWNPYLMNGSWTTNPAP
                     DARFPLVIDAVRLAYGDLYIGGNPASTFNALMANFRRGMRYLSAFDNQPGKWKDFDFL
                     TLSRVTGEHSLRIRQTALSLWSIAPSAILLDLVTPDMLPLLTNRAFLAVHQDPLCAPG
                     RQVASNATCEVWMRPLVSGAKALCVFNKTASPQTNIVTFSSLGLTPGPDCYARVYSVW
                     DNQTLYYATNDFSINLTNYYANLLVVSPNRLAAAASFLPTTNLPLPGATWTLRSDGTH
                     LYWSSP"
     gene            complement(40473..41003)
                     /locus_tag="PKI07_03245"
     CDS             complement(40473..41003)
                     /locus_tag="PKI07_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOC50097.1"
                     /translation="MTTPRSQMLDFILGRRSIRIYSPGEVDDEVVTRLLEAAMAAPSA
                     MTKDPWRFVVVRDRQMLAKLATLHPGAAMLSAAAMAIVVCGDLDVAFERQLSYLLQDC
                     SVAIENLLLAAHAHGLGACWVGIHPVEPLIQRVKELLSLPASFVPVAAISLGQPGEAP
                     PPRTRFNADYVRQEKW"
BASE COUNT         7040 a        13251 c        13698 g         7588 t
ORIGIN      
        1 acgcggaaga cggggttcgg ctgcggctga atgtcgggga ggcccatgaa gacatcatgg
       61 tctttgggca gccaccctgc agcgcgggcc gaatgaaacc ccgccgtgcc tattacctgg
      121 gcctgttggg cccaagccag aacgggcaaa gcgacatcac ggacctctac gccgcccggt
      181 ttgggcgacc cgccccgggg cagaaggtgt ttatcgccgt ggttcagacc cggaacggct
      241 ggaaaggcca aagccgggtt cacaacgcca tcgttccgcc gccgccgatg ttgaaatctc
      301 agcaagcggc tgaaacggca agggttaaga aatcgaagcc cgcccccgcc ccggcatcca
      361 ccaccgcccg caagaccgtc tcttctccgt cctttcgagt catgtacaag gggagtacat
      421 cggaagcccg gcaggagcac atggaaaaca agcatgagcc ccctttgagc ctccgttgta
      481 cacccgttgt acacggccta atgggagcgt tgggctggct ggggcgggtg gggcgggttt
      541 gggcgcgggc ctgagccgaa atggggggat gaacgagtcg cgaacgatgt aaaacgacgt
      601 taaaacccag gttgagcccg gctcgggcgg gcggttgttg aactcactgg ttagtgagaa
      661 aggagtggtt gtctcctgcc ccggggtctg ggcgggcggg ttttttctgt ccggcgcgga
      721 tcataagagt cgagcggtct gctggccttt ctttccatcg gggacggaat tgggactggc
      781 ccatcaattc aagctccgct gaggaaggag ctccgcaaaa cggggcgagc ttgcaggttt
      841 aagcaggacg ccacccgagg ttcccgggaa ccggcgccag ccgaggggcg ggaaaaccca
      901 ttggccgtct ccggcgacgg gcggcggttc ctgacctttg cgttgagttt tacctgcccg
      961 caacgcgcca tcggcggcca tgacgcggcg cgaacatgcg ttgacgccgg ccatgggatt
     1021 gtgtttagtg gcgcgtagat ttgaatatcc gcatgaagac tttatttttc cttccggccg
     1081 ggggcctgcg ggtggcggcg ggtgtctgcc tggcggtgtg gctgaccggg tgcgccacgc
     1141 agcgcgtgga ctggcccgcg cgcattgggc aatataccta cgaccaggcc gtcgcggaga
     1201 tggggccgcc cgagaagcag gcccaactgg atgatggccg ccgggttgcg gagtggctcc
     1261 tgaaccgggg cagcacttac gtccaggcca ccccgcacct ttatgggccg tattatgggg
     1321 ggttcaccac cgtgcaaacg gcgcccagcc ggtttatgcg gctgaccttt gacaccgccg
     1381 gccggctcgc cgactggaaa aaggtttacc ggtagggccg gcggaacgcg ccgcgccggg
     1441 aggcgaaccg ccgcgccaac gccgcgggcg cagactccgc gcttgcaact cccgggcggc
     1501 ttcgtcaggc tcccggccgg ctcgccgtcc ggtccggagc gcccccgccg cgccgcgagc
     1561 ggacgaccga atgatgaaga cttgccagcc gctgatcgcc gagctccagg agctgtaaac
     1621 ggacctgtgc ggccgggact ttctcctgac ctgggaaaag accgacgccg agatccgcgc
     1681 gttgttgctg gtggccgaat gcctggcgga gctgcaccgg gcgcgccagc cgttccgggt
     1741 gtttgactcc gggctggccg tctcgatttt ccgggacaag tccacccgca cgcgcttcag
     1801 ttttgcctcg gcggtgaacg cgctgggcct ggggctggcc gaattggacg aggagaaacc
     1861 gcagttggcc cacggcgaaa ccgtgcgcga gacggccaat ccatagccgc gggcgactat
     1921 ctgaagcggc gctttcccgc gctcaagacc gtggccagtg aagcctggca atgccccacg
     1981 ttgctgctga acggctttgg cgagcatcgc atcgagggca ttggcgacaa gcatgttccg
     2041 tggggccaca acgtccgcga caccgacgcc gtggcggcca ttgatgacga ggattgcctg
     2101 cggattctgc gcctgttcaa cgagccggcc gggcgggagc acctggtggc gcggggcgtg
     2161 ggcgagggcg aggtggaaca gctgggctgg ctgggcatct ccagcatcgc caacctgctg
     2221 gccgccatca aaacggccaa gtacttcgag ttgggcgagg cggacctgct gctcaccgtg
     2281 ttcacggatt cgaccgatct ttacgcgtcg cgcctggcgg agctgcgcga gcagcggggg
     2341 ggcttatacg tagaaagacg ccgtggccga ttatgccggc ccgctggcgc accagggccg
     2401 ggacggtttc aaggagctga cgtacccgga gcggaaggcc attcacaatc tcaaatacta
     2461 catgtgggtc gagcagcagg ggaaaaccgc gcaggaactc aacgcgcaat gggacccgga
     2521 gtattggcgc gcccgtttcg aggacgaagt ggcgtatttt gaccagttga tcaccgagtt
     2581 caaccggcag gcgggggcgg cggactgacg ggagcacgcc ggcgggaacg gcggggcggg
     2641 ggttgcggcc gggcttgacg cggggggagg cgaagggtta tttctaggcg cagttatgga
     2701 cgtgttgctg ctttcgcgaa tccagtttgc gctgacgacg tcgttccatt acctgtttcc
     2761 cccgctgagc atcgggctgg ggctgctgct ggtgattatc gaggcggtct ggctcaagac
     2821 ccgcaatccg ctgtatcacc agatggcgcg gttttggacc cgggtctttg cgctgacgtt
     2881 cgccatcggc gtggcgacgg ggctggtgct ggagttcgag tttggcacga actgggcgac
     2941 ctactcgcgg tttgtggggg atgtgtttgg cagcccgctg gcggcggagg gcattttcgc
     3001 gttcttcctg gaatcggggt tcctggcggt gctgttgttt ggctgggacc gggtgagccc
     3061 gcggctgcat ttcttttcca cctgcatggt gtgcctgggg gcgcatttca gcgcggtctg
     3121 gatcgtggtg gccaactcgt ggatgcagac cccggccggg tttcatattg tcggggaagg
     3181 gctgcgggcg cgggcggaga ttacggattt ctggcagatg gtcttcaatc cttcggcggt
     3241 ggatcggctg tcgcatgtct tgtgcggggc gtggcaggcg ggggcgtttc tggtggtcag
     3301 cgtgagcgcg tggtatctgc ttaagaaacg ccacacggag tttgcccggg cctcgctgcg
     3361 gatcgggctg ctgacggggc tggcggcttc gctgctgcaa ctggccaccg gccatagcag
     3421 cgcggcgggg gtggcccgga accagccggc caagctggcg gcgttcgagg ggctttatga
     3481 caccatgccc cgcgcgccgt tgacgctgtt cggctgggtg gacgagcggg cggaggtggt
     3541 gcggggcggg gtggcgatcc cgggcctgct gagtatcctg agctacgggg atatccacca
     3601 cccggtgcag ggcttgcgcg agttcaagcc ggaagaccgt ccgccggtga acctgtcctt
     3661 tcagttctac catatcatgg tgggcgtggg ggtggggatg atcgcgctgg cggggctggg
     3721 ttgctggtat ttctggcgcg ggtccctgcc cgggcagcgc tggttgctct ggctgctgac
     3781 gctgtcggtg ctgggcccgc tgctggccaa tcaaaccggg tggtttgccg ccgaagtcgg
     3841 gcggcagccg tggctggtct atggactgtt gcggacgtcc gacgggctgt cggcagtggt
     3901 cagggccaac accgtgctga catccattct cctgttcagc gcggtttacc tgctgctgtt
     3961 cgcggtcttt gtttatctgc ttcactccaa gattcagcac gggccggacg cggcggattt
     4021 gacgccggcc ggaaaactgg cgcggccgcc gcgcgcgaac tgaaagaagg gcatgagcga
     4081 cactgatttc atgagtttca ccgacaggcg cgaagtatga gcggcgcggg actggatctt
     4141 aacgttgttt ggttcgccct ggtcggcgtg ctttttacgg gctacgcggt gcttgacggg
     4201 ttcgatttgg gggtgggagc gctgcattta ttcacccgga cggatgagga gcggcgggtg
     4261 atgatcaaca ccatcgggcc ggtgtgggac ggcaacgaag tgtggctggt gaccggcggc
     4321 ggggcgctgt tcgcggcgtt tccgaacgtg tatgcgacgg ttttttccgg gttctacctg
     4381 gcgctggtgc tgctgttgct ggcgctgatt ttccgcgcgg tggccattga gttccgcagc
     4441 aagcagccga tgcgctggtg gcggcagatg tgggatttcg ggttcagcgc ggggagcctg
     4501 gccgcctgcc tgctgttggg agtggcgatt ggcaatattg cctggggcat tccgattgac
     4561 gcccggggcg aatttgccgg cactttctgg ggcctgctgc atccctacgc gctcctgctg
     4621 ggcgtgaccg cggtggcggt cttcatgacg cacggcgcgc tttacgccct gatgaagacc
     4681 gagggggcgc tccacgacaa actgcgccgc tggagcaaac ggtgctttat ccttttggcc
     4741 gtttgcagcg tggcggccac gctggccacg ctgttctgcg tcccccacct ggcggcgcgg
     4801 gcgcgggcga atccctggct gttcggcctg gtgctggtga acctgctggc cattgccaac
     4861 atccggcgcg aagtccagcg ggggcgggac gggcgggcgt ttttatcttc gagcattggg
     4921 atcatggccc tgatggggct gttcgcgctg gggatgtttc ccaacctgtt gtggagccat
     4981 ccggacccgg cccacaatct gaccatttac aacgccgctt cctcgccccg gacgctgggg
     5041 atcatgctca cgatcgcggc gatcgggatg ccgttggtgc tggcctatac cgcgtttatc
     5101 tactggattt tccgcggcaa ggtgcggctc gaccgggcga gctattgacg ccgcccgcgg
     5161 ctcgccgcgg cgggggcgcg cccagcccgg cgcgcggttt gcggggtttg gcgcgggcta
     5221 cttcaggtta atctcaacca ggctgaggcg ctgattggaa cgcacatagc cgaagatcag
     5281 ctcgaacttg ccgtaatgcc cggtcaggat gttgctcgtg cggccccggc gggtcagcgg
     5341 gagcttttga atggccgcga gcgagagcga atccgtgtcc gggctgattt cggtgttttc
     5401 caccttgagc cggccggcaa acggcgtggt ggcgccggcg ttgccgccgg tgccttcgca
     5461 atccagcacg atgctggtcg gcccgccgtc cggctgggca tagagcagca gcccgtggcg
     5521 gtcgaacacg taaataattg ccccggtttg cgccacccgg ttggtgcggc tgggggcgcc
     5581 caactgccgg gcgacagcgg gcaaaagatt ttcccgcgag acccgctcgc cgccgaggta
     5641 caggccgtcc ggctgcagtt ccagcagcac gggcgccgcg gtattgcccg gcgccgcgcc
     5701 gccggcttcc cccggcggga cggggcgcgg cgagttgtcc cggggagttg cggcggccgt
     5761 ttcggagcgc ggcggggaga agggatcctt ctgttggcgg accaggatgg caatgacgat
     5821 cgcctgcagg gccagggcca gggccaacag ggcgataaac agcttccagg gaaaccggcg
     5881 ccgcccgggg cgcgcggccg gcggggctga cggtttccgc cggggcgctg attccgtgga
     5941 actcccggag gggccatttg ccgtcccggc tgcattgctg cgtgatcgtg accgccgccg
     6001 cggcgtaaac tccagtgacg cgttggcgtc ataggaaacc ggtctgccgt cgcgcagatc
     6061 actgaccaat tgggccgcct tgtccgggtc caggccgcgg cggatcagcg cttgaacgat
     6121 ttcggcttcg gccggatttt cgttcagcag ccgacgggca aacgcgacgt ccgattgcaa
     6181 aatatcacgt atcatgcctg cgcgagcatc aattaccacc ggtcttgcgc cgattcaagc
     6241 tcacaattat aaaaccgcgc ccgcgccggg ccgggtggga gcccgcgcgg ttcaatactg
     6301 ccgttttccg ggcaccaggc cggcccggtg cttttcgagt tcctgccgca gatcggcgat
     6361 gatttcgggg tgatcggcgg ccacgttaaa gcgttccccc gggtcgttgg gcagatgaaa
     6421 cagcagcggc gggtcatggc gctccggggt atcctcgcca tagccggaat aggtggtgaa
     6481 gtgcgctttg taggccccct tgcggagggc atagacttcg tcgccgttgt aatagatgac
     6541 gtggttgcgc tggctggggc cttggcccaa cagcatcggg gtcatatcca cgccgtcaat
     6601 gatgcggtcg gcgggaaggg gcactccggc cagggccagg caggtgttga agaggtccat
     6661 cgagcaagcc agctcgcgat tgacggtgcc ggccttgatc cggccgggcc accaggctat
     6721 ggccggctcg cgcatgccgc cttcccaggt gctgcctttg cccccttgca ggtggccggc
     6781 gcagccgccg acgagttctt tgatcagcca cggcccgttg tcgctcgtaa aaaagacgaa
     6841 cgtttgctgg tccaacttct gctggcgcaa tgtttcgagc acccggccga cactccagtc
     6901 cagttcctcg accacatccc catacaagcc gcgcggactg gtcttttgaa atgccttgga
     6961 ggcaaagagc gggatatggg ggtaggtgtg cgcgaagtag aggaagaagg gcttttcccg
     7021 gttctgctga atgaagcgca cggccttttc ggtatagcgc cgggtgagcg tgctcaggtc
     7081 ggtcggctgc tcgaggggct ggttgttgcg catcaaggtg caactccacc attcggggtt
     7141 ggggttgagg ctgctggaag cgcccttggg gatgccgcgc gtcggttcca tgtcattgga
     7201 ccagcgcagg ccataaaagg cgtcgaagcc gtggcagagc gggaggtttt gctggtagtg
     7261 gccgagatgc catttgccga cgcagaacgt cgcatatcct ttctccttca gcgcgtgggc
     7321 gatggtgatt tcttccgggg gcaagccgcc gggcgatttg cggctttgca cccggtgctg
     7381 caggtcgccg gacatgccgc tgcggatggg cagccggcct gtgagcagcg ccgcgcggct
     7441 gggcgtgcaa acgcaggcgc cgacgtagaa atcagtgaac cgcattccct cggcggccat
     7501 gcgatcgagg ttgggcgtgc gaatgctggg atgcccgtag cagccgaggt cgccgtagcc
     7561 caaatcgtca gccagaatga tgatcaggtt ggggagttgg gcggcggggc ttgaggcggg
     7621 cagggcggcc aggattgccg ccattccagt cagcaagaga ttcaagatat ttccgcgcat
     7681 aagcgtccga ctgacttcgg caaggccatg agttagcaga gcgcggcgtt ggtggcaacc
     7741 caaacctggc cggcgcgcgg cccgccgtat tcgctactta tccggtccgg cattctttag
     7801 cggccggctc tttttccctt ttttgcctgg ggcgggcggc ggagagccgg aatttcgggt
     7861 gggcattggg gcgtggatgg acttgcgcca ggcggccagc cgggcctgca gttcccgggc
     7921 cgtgtccggc agtttggccg cgaggttgtt ggtctggccc aggtcggtgc gcaggttata
     7981 cagttccagg cggccgtcct cgaagtattc ttgcagcttc cagtcgccgg accggattgc
     8041 gccggccggc gtggtccgcc acgcgcccgc gcccgcgccc aggtatcccg ggaagtgcca
     8101 gaagatcgcc tcgcggcgca gcccggtctt gccgccgctg gtcagcgcgg gcaaatagtt
     8161 cacgccgtcg agcggataat cgggcggcgg cgaggcaccc gccaccgcca gcaaggtggg
     8221 atacaggtcc acgctgttaa tgggctggtc gcagacggtg cccgccggga tgcgccccgg
     8281 ccagcggaag atatagggca cccggatgcc gccctcgtaa agcatgcctt tgccgccgcg
     8341 caacggcgcg ttgtcggtca cgtcgccggt cggtttgatg ccttcgcgga cgtagccgcc
     8401 gacgccgcca ttatcgctgg agaagatcac cagggtgttt ggcgcgagcc gcagttcatc
     8461 cagcagcgcc agcacccgtc cgacgctctc atccacgctg gcgatcatcg cggcgtaagt
     8521 tgggttattg tgccccccga cggtcggttt ggcctggaac ttcgcgacca gggagtcctt
     8581 ggcctggaga ggttggtgaa cggcgtaatg cggcaggtaa aggaagaaag gctgctcctt
     8641 atgccggcga atgaaatcca ccgcccgatc cgtcaggaaa tcggccaggt aagcgccctt
     8701 ggggtattca accggcggct gcgtggtgaa ctcaaaatgc acgccggcgc tgacaatcgc
     8761 ctcatcaaaa ccgcgctggc gggggtggta acgctctccg tcgccgaggt gccacttgcc
     8821 gaacataccc gtcgcgtagc cggcccgctt cagggactgg gcgagggtga ttttatcgag
     8881 aggcagccgg atgacgttgt ccaccggccg cagcgggcgg ctgcgccagt tgaaccgatc
     8941 gatgctgccg acggtataga cgccggtgcg ggggccgtat tggccgctca tgagcgcggc
     9001 gcgggtgggg gcgcagtttg ggccgcagga atagccgctg gtaaatcgga tgccgcttgc
     9061 cgccatccga tcaatgttgg gcgtttcgta atacttgctg ccataacaac ccacgtccgt
     9121 atagccaagg tcgtcggcga gaatgaaaac gatgttcgga ggccgatccg ctccgcgact
     9181 cgaaaaggaa cccgtcagga ggattaacaa ggcgaggcag gccccggcga cgcgaagggc
     9241 gcgatgatgg ctattcatgc cctatgctgt ggggagcgcc cttgattagg caaaccagaa
     9301 atggcgttgc gctggcaagc cctggttttg aagcacttgg gctggtgagg ccggcgggac
     9361 gattggctgg ccgggttgga cggaaacgaa cgcaatggca ggtttccggg ttgattgggg
     9421 cgcgattggc gggcgagcga ggaatgcttg cgcacattgg cgaatctgca aataatatgg
     9481 gtgcgtagcg tcgcgcaacg ggccgggagc tttcggtgcg cactggagag tcggattgga
     9541 atcgagtttt tgcttatgcg aacaatgcac agaatgagtt ggtgggttga ttggggccgg
     9601 cgtcttggcg cggccgggtt aggcatgtgg gtggcgggag ccgcggcggc cggcccgggt
     9661 ttgacgcctg cgcagcttcg ttgcgagtat cgcacgaacc cgctgggcat tgatgagttg
     9721 cggccgcggc tcagttgggt ggtcgcatca ggcgagcgag gccagcgcca aacggcttac
     9781 caggttctgg tcgccagcga tgaaacccgc ctgcgcaaag gccggggaga tttatgggac
     9841 agcggcaagg tggccggcgg cgagacgacg ggggtggttt acagcggcaa gccgctcggc
     9901 tcgcggcagc tttgtttttg gaaagtgaaa gtgtgggaca aggatggccg ggcatccgac
     9961 tggagccagc cggcccagtg gtctatgggg ctgctgaaag acaaggattg gcaggcgcag
    10021 tatatcagct tccgcgacac cagcccggtg cataagttca aagaaccgct gttcctgccc
    10081 ccggcccggc agtatcggaa ggaatttgcc gcggcgcgcc cggtgcggcg ggcgaccctt
    10141 tatgcgaccg cgctgggcat ctatgagttg cacctgaacg gccagcgcgt gggcgacgag
    10201 tggtttgcgc cgggctggtg cgattatcat cagcgggcct attacaacac gtatgacgtg
    10261 accgcgctgg taaagcgcgg agccaatgcg ctgggggcct gggtggcgga tgggtggtat
    10321 tcgggttata tcgggtttgg cctgctgacg ggcatcggga ccgaggccat tgggcgttat
    10381 acttacggga agaccccggc gctgcaggcg cagctcgaga tcgagtatgc cgacggctcg
    10441 cggcaggtgg tggcgaccga cgcgtcgtgg aaagtgaccg gggcagggcc gatccaggag
    10501 ggcgatttcc tgatgggcga aagctatgat gcccggcagg agatgcacgg ctgggcggag
    10561 gcggggtttg atgactcgcg ctgggaagcg gcgattcgcg ccgaggccaa cggaagcgcg
    10621 ccggcgaagt tctatgagta tcggaacccg acggggggcg gcggcccgaa gattgagggg
    10681 cgcgaggtgg acctggcctt caaaccgccg ccgaagcggg aagcgttccc aggggtgccg
    10741 gtgcgggccg tcgaggagat ccgccccacg ggcatcactt cgcccaccaa tggcgtttat
    10801 attttcaacc tggggcagaa ctttgccggg gtggcgcggc tcaaggtcaa aggcccggcc
    10861 ggcacgcggg ttcgcctgcg ctacggcgag atgctgcacc cggatggccg tttgatgacc
    10921 gagaacctgc gcaaggcccg cgcgacggat tactacacct tgcgcggcga cccgctaggc
    10981 gagacgtatg tgccgcgctt cacttttcac gggttccaat atgtggaact gacgggctac
    11041 ccgggcgagc cgggcctcga ggcggtcacc ggcattgtgc tccaatcgga taccccgctg
    11101 gccagcgcgt tcgaatgctc cgatcccgtg gtgaaccggc tgtttaagaa tatcgtctgg
    11161 acgcaacgcg ccaatttcct cgatttgccg acggattgcc cgcagcgcga tgagcgattc
    11221 ggctggacgg gagacgcgca gatttatgtc gcagcggcgg cttacaacgc cgacgtggcg
    11281 gccttttaca gcaagtggct gcgggaattg atggaagcgc agcggccgag cggggtgttt
    11341 ccgggctatg cgccgtatcc gtttcagcat ggatgggatt tcggcacggc ctggtgcgat
    11401 gcgggcgtaa tctgtccctg gacgatttgg caggcgtatg gagacacgcg aatcatcgag
    11461 aggtgctggg aaccgatgac gcggtttatt gcctggcgca aggccgcgag ccgggagttt
    11521 cttggggtgg cgcacggcaa cgagtggggt gattggctgt cggtgaatga gaagacgccg
    11581 attgactata ttgacaccgc ctacttcgct tactcgacaa agctgatggc ggagatggcg
    11641 caggccatcg gcaagactgc ggcggcggcg gattacagcg cgctgctggg gaagatcaag
    11701 gccgccttta accggaagta tgtgcaggca gacggcgcgc tgtcggtgga tacgcaaacg
    11761 gcctatgcgc tggcgcttta tatggacctg ttgcccgagt ccctgcgcgc ggcggccggg
    11821 gcgcggttgg cgaagaagat cgaggctaac gagggccgga tgagcacggg ttttttgggt
    11881 acccggccgt tgttgcccgc gctgtcggcg accggtcagt cggacctggc ggcgcgcctg
    11941 ctgcaaagcc gcaaatatcc atcctggggc tacgaggtcg agcagggcgc gacgaccatc
    12001 tgggagcgct ggaacagtta taccaaagac aaaggctttg gcggtgcgca gaatgcggag
    12061 atgaattcgt ttgcgcacta ttcgttcggc gcggtctgcg aatggatgtt ctcgcaactg
    12121 gccggcatcc agagcgacgg gccgggctac cggcgcatta tcatccggcc cacgccgccg
    12181 tcgccaggca gcaacccgga tcaggcgccg attgactggg tgcgcgcaca ctatgattcc
    12241 atccacgggc gcattgccag cgcgtggaaa gtcgagggcg agcgtttccg cctggaactg
    12301 gctctcccgg caaacaccac cgcgacggtt tatgtgccgg cgcgcgaggc ggcgagcgtc
    12361 acggaaagcg gccacttgct ggagcaatcc cccggggtga ggttcctgcg gatggaaggc
    12421 aaccaggcgg tgctggcggt tgggtccggc agctaccagt ttgcctcacg gctttgagcg
    12481 ccggttttgc ggttttcccc gcctgtctgc ccggcatccg ggtgggcgga atcgcgcatc
    12541 ttcgcccgca ggttggctcg gagtggatgg agagacgcgc tggggcagag gcaagggccg
    12601 tggttatgat caacgccgct gaaaaaccaa cccgcgggcc ggtccagtgg gacgggcgct
    12661 ggtttttccg agggctgcaa aaaaggcgtc ccagggggac gcctttgaca ccggggcagt
    12721 tcgattggct caggcagccg cctcgccgat ctgaaaccgc acaaagcggc gaatagtcac
    12781 ttcgtccccc aactgtttgg cgacggaggc aacgtgctct ttcacggaga tttccgagtt
    12841 ctgctttacg aagccctggt ccaccaggca atagccttgg tagaacttct ccagcttgcc
    12901 ttcgacgatc ttggcgaccg cttgcggcgg cttgtttttg aactgttcgg cggcgatttc
    12961 cctttctttg gccagcacgt ccgccgggac ttgttcgcgc gatacggtga gcggattgcc
    13021 ggcggcaatc tgcagcgtaa tgtccttgac caactgtttg aagtcgtctt tggccagcgt
    13081 ttcttcccgg cctgcgccca cctctgccag cacgccgacc ttggcgccgg tgtgaatgta
    13141 ggcggcgatc atgccattgc cagcgacctc cattcgcgca tgacgcgcga gtttgatgtt
    13201 ttcgcgaatc tttgccacca gcccttcgcg ttcagcttcc aggctcgcct gggggtccgc
    13261 cgccagccgc cgcgccactt catcggcgaa agaacgaaac tcctcgttgc gcgccacgaa
    13321 atcagtctgg cagttgactt ccaccagcac gccgacgcgc ccgtcgggtg cgacatatcg
    13381 ggcgataacg ccttcgcggg cttcgcgcgc ggcgatcttg ccggcagtca ccgtgcccgc
    13441 tttccgcagc agatccaccg cggtgtccaa gtcgcctttg gcttccgtta acgccttttt
    13501 gcattccatc attcccgcgc cagtcatccc gcgcagtttg cccaccaggg ccgctgtgat
    13561 ctcagccata ttaacagtct ttccttagag attgaacgtt gttcgttgtt tggatgaatt
    13621 gttaggtggt gcgggcgccg tattgcgggg gcgccacgct tcccaaaatg gtcaggccgc
    13681 cgccggggcg gctgtatcag ttgccgagac ggaaggggct gcttccacgg ggaccgccgg
    13741 cgcaggttcc ggttgggctt cgggcatggt ggccgcagct tcctcgtctg ctttgcggcg
    13801 cgcgtgtttg gtttcgtact cggcttgcgc ctgcatcacg gtttggccca cctgcgccag
    13861 gatgagccgc accgcccgga tggcgtcgtc gttgcccgcg atggggagat ccgtcaggtc
    13921 gggatcgcag tttgtgtcca cgattgccac cacgggaatc ttgagccgcc gcgcctccgc
    13981 cacagcgtta tgttcgcgct tgatgtccac cacgaacagc gcgcccggca acttgtccat
    14041 cgtctggatt ccctcgaaga acttcttcag ccgcgctgcc tcgcgccgga tgactgattg
    14101 ctcctgtttg acgaaattat taatcgagcc gtcggcctcc atcttctcga tttccttaag
    14161 gcgcccaatc gagcgcttga tcgtcgcgaa gttggtcagc gtgccgccca gccagcgttc
    14221 ggttacgtag aactggccgg tttccttggc ggtttgttta atggcttgtt gggcctgttt
    14281 tttcgtcccc acaaacagca ctttgccgcc tttgcgcacc gtggcgccca gaaaattaca
    14341 cgcggcttcc agttgattga tggtcttgcc cagatcaatg atgtggattc cgttgcgggc
    14401 atcaaagatg aaaggcttca ttttggggtt ccaccgtttg gtctggtgtc caaaatgcac
    14461 cccggcgtcc aacagttctt taactccgat cgcgatcatt cgtttccttt ggttaaaagc
    14521 cggccttgcg ggccagccat cccgcggaac acgggattga tttatatgtc gttatggccc
    14581 cctgcgccca gcggcacagg catttaaggc cgtttattcc ccccgtccgg caggacacgt
    14641 tacacccggc catttccgcg aggaatggct aacataacaa cttcacgccc cgccgcaact
    14701 gctattttta cccaaaaaaa tggtggggcc gcgcccaagg ttaaaagtga accggttggg
    14761 agaccgctcg cgccaggcgc tgtaaatact ccgcgcgcgg gatggtgatg ccgcccagtc
    14821 gggcggtgac gggcgtgagc atctggaggt caaacagctc gaatccgcgc tgccgcaagt
    14881 gttcgacgag atgagcgagc gccactttcg aggcgttgct cacccggtga aacatggatt
    14941 cgcccgcgaa caagcccccg atcgtcaccc catagatccc gcccacgagc cggcggccct
    15001 gccagcattc caggctgtgg gcgtgaccct gcgcgtgcaa ctgcgcgtaa gccgcgatga
    15061 attcgggggt gatccacgtg ctgcgccgcc cgggcgctgg agccgcgcat gctttcatga
    15121 cgcgcgtaaa tgcctggtcc cgcgtaatct gatatgtccc ctggcgcaag attcggccca
    15181 ggctgcgggg cacgtgaaag ctttcccacg tcaatatggc gcgtgggtcg ggcgaccacc
    15241 acgtaatcgg atccacggtc caggggaaga tgccgctgcg gtaagccagc agcaggcgcg
    15301 gcacggacaa atctccgccg accgccacca gcccttccgc gtccgcgccg cgcgggttgg
    15361 gaaagtgcaa ctggtgatcg agcagggtga agcgcatcgt gagtgtgttt ctttggccgg
    15421 tggccagccg cgcggcaagg gcggggcagg cccgctcgcc gccggcatcg gcaccgattt
    15481 cattcttcct tatgcagcgc cgcgagcaat tgcaactcaa agaagccagc gatctccatg
    15541 aacaggagat cgcggacgga aacgtcgtcg agtttgggcg gcgtcgcttc cggctcgttg
    15601 agttgaatcc agcttccgat tcggatgtcg ttcagcactt gcaggagcca ttcaacttca
    15661 gctggcgcca gtgagaggcg gcctcctgtc tttgttggcg caaaccgctg ccgatccgcc
    15721 agcagcgcca gtacctgttg ccggttttct gcccgctgtt cggcgagggc ttcgttcagc
    15781 aattgctgat tcgcctccgg gtgaggaatc cggccagtcc ggctgagcgc ctggtgcgcg
    15841 ggcggaatgc gcggatacaa ctgcaggatt tgcggcagca ggaatgcctc ctgccgatcg
    15901 aggtgaaaca gcagccgtgc gccgggggtt gggaccagct tcattcgtcc cgctccatcg
    15961 tcgcgtgcag cgagtatttt tggagttgat gcacgtaatg ctccgccttc tccagactct
    16021 cgcgtgccag cacgctcttg ccctgctcat gcacctgccg catgtgaaac tccgccttgg
    16081 cgcgcgccca gccgaagacc ttctgaaaga catgcgtgac gtagtccata taattgaccg
    16141 agtcattcca gcaaatgacc aggtagcccg ggtccaggct ggactttggc cgatcctcgg
    16201 tcgtttggac caggccaggc tgaatttccg gcgttgctat tcccgccatg atgccatctt
    16261 accaaaggcg gcgcggcttg tcgagccacg ccgccggcac ctcacagttt tgggcaacat
    16321 actcaagagg ggaaccgagc tatcgtaggc tccgatgctt caaacggcga ttacgctgtg
    16381 accctctcgg ttttggaact ccccgcacaa cggcagccat ccgtttgaag cgctcttcca
    16441 tagacgcttt ccaggcttac cctcactctt gcaaataccg cgaatcaggg cagacatgag
    16501 cgcctgtcct gccgatctaa caggggaggc gcgccaccat gccgcggtac cggggcgtgg
    16561 gagttcccgt ttattgctgc gaggcactga gtgcctggtc aatatcggcc tggatgtcct
    16621 cgaggtcctc gagacccacc gagacgcgga cgtagtcggg cgtgacgccc gccgccgctt
    16681 gttccgccgg ggttaactgc tgatgcgtag tggaagccgg gtgaattacc aggcttttgg
    16741 cgtcgccgat gttggccaga tgcgagagca gcttgacggc gttaatgaac ttccggccgg
    16801 cggccaagcc gcccttgatg ccaaagccca ggatgccgcc gaaaccgtgt ttcaggtaac
    16861 gggcggcgat ggggtggttg gggttgtccg gcaggcctgg gtaaatgacc cagcttacca
    16921 ggggatgctg ctggagccag cgcgccaggg cgagcgcatt ggccgagtgc tgctgctggc
    16981 gcagcggcag cgtctccagg ccttgcagga acaggaaggc gttgaaggga ctcaggcagg
    17041 ttccgaggtc tcgcagcagt tgcacccgcg ccttgaggat gaaggccaca ttgcccatgc
    17101 ccggcacgtt ggagaaggcc tcccagattt tcaggccgtg atagctcggg tccggttcgg
    17161 tgaactccgg gaacttcccg ttattccact ggaacttgcc cgaatccaca atcactcctc
    17221 caatcgaggt gccatgcccg ccgatatact tcgtcgccga agccaccagg atgtcggcgc
    17281 catgatccag gggccgcacc aggcccacgc cgacggtatt gtccaccacc agcggcaggc
    17341 cggcgtcatg ggcaatttgg gccagcgccg cgaaatccgg cacgtccaac cgcggattgc
    17401 cgatggtttc gacgtaaagc gcgcgggttt tcgcggtgat cgcctgccgg aacgcctccg
    17461 gtttctgcga gtccacgaat ttcaccgtgc ggcccagctt gggcagtgtg tagtgaaaaa
    17521 gttggtaggt gccgccatac aggttgctgc ccgacacgat ctcgtcgccc aactgcgaga
    17581 ggttcagcag cgtcagcgtc gtggctgtct gccccgaggc caccgccaac gcgcccgtgc
    17641 cgccttcaag ggcggcgacg cgctgctcga acacatccgt ggtgggattc atgatgcgcg
    17701 tgtaaatgtt gccgaactcc ttgagcgcaa acaggttggc cgcgtggtcc gaatctttaa
    17761 agataaacga tgtggtctgg tagagcggca ccgcgcgtgc gccggtgacc gggtccgggg
    17821 tctggccggc atgcagcgcc agtgtgcgaa ggtttttaga cgtattattc ataagcttgg
    17881 ggtataataa acttctattg ccattatgca agcatccgga aggatatact cctgtcgagg
    17941 gtcgcccact tgggtttttg gcgaacggac tgattccgtg gcggctttgc gaggtgggct
    18001 agcgggcgcg gtttttgggt ttttgcaggg cgtggccgta ttggcgtttg aagctgtggc
    18061 aggaggcgaa gtcggttttg ccgtcgtcgt tctcccagac ttcgtcaaag cgagcctcgc
    18121 gtggcgggcg gcgcagggtg ctttgccgca ggatgttgcc ttgctcgtcg caggtgattt
    18181 cgatgaagcg cggcttttgg agccggttca ggcggttgga caggatgacc aatacttggc
    18241 gtttcatagt cggctatttg cagcgcacca gcacgcgcag ccgcttggtt ttgtaggtgc
    18301 ctgcgttggc ggcgaaggtc cagtcggggt tgccttgggg ctggttgccg atcccgaggt
    18361 cggcgcggct gccttcgttc caatggttca gggcgaagag cgtctggcgc gcttcgtggt
    18421 tatgcacctg catggagccg tagcccgcga cttcggctga cggggcgtcg ccgaagtccc
    18481 aggtttgcgc ggaggcgtgc ggcacgttgg cggcgttggc cgggccgtag ttgttgggcc
    18541 agaactcaag gttgccaccg gccagacccg tgccggtcac gatgcccttt acgttggaat
    18601 agacgttcag gttggcaatg ttttgctgga aatgcgcgcc cgttcggcct cgcggaacac
    18661 caagcttgcc cggcgcgtcg gtgaaggcat ccatcgagac atacaggtat tgggtggggc
    18721 ggtcggcggg ttgcaactcc aggaagtaag ccacccggtc gaagggccgc tgaatcttgc
    18781 tgctgttatc cacttcatag acgaagtccc ggcccagctt gttcaggtct aggtcataca
    18841 ccaactggta atctttggcc tcgggcacct tcaactccag ccagtcgcgt ttgggggctt
    18901 gcccggcgcg gaaggcgcct gcgggcaggc cggcggcgtt catcaggttc ggctcagcca
    18961 gcatgtgcca ggcaaaacgc atggcgacgg ggtgtttcac ctccggggcg cgcaacacca
    19021 cggaagcacc gtctatgcgg gcatcggctt taacgaagcc accttggtcc gcatcaatca
    19081 cctcgaacca gttcagcggc tgaccatcgc gactgaccaa cccgccaccg acatggtcga
    19141 aggtcacgcg cagggcgtcg ccttccaggg ccaaggactt aaacgtcggt ccggagtaag
    19201 ccacggcttc ctgtccgtag gtccgggcca aggcccacag cgccaaccgc cgccccacct
    19261 cttgcttgtt ggtgggatga atgtccttga ggtcgctgat gtcgttaatc acggccatgc
    19321 cggtgaaggg caccgcctgc gccgctgtct gcgcctccca gaactccccg agggtttccg
    19381 ggtcaaagct gtaagtgaac ggcgcgattt gcacaaagta gaatgggaaa tcgccctgcc
    19441 cccaaacctg ccgccagccg cggatgagcg ccttcatccg ctccgtatac aacttgcctt
    19501 cggtggcgtt ggattcaccc tggtaccaga ttgccccacg caatgggtat gggtgcagcg
    19561 ggtggatttg gccgttgtat aacgcggtcg gatgctgcaa atcacgcgga ggtagcagct
    19621 cctcggggta gactggcatg gccgggacaa gctggcgatc ggtcagtgcc tgccgggcgg
    19681 acgccatcca gcgcccaacg acttgcagaa attgctccag ccgctgctga tggagttggg
    19741 tgcgcggatc gcccagttga acttgttcgt attgcgtctg caatgccggc accgcggcaa
    19801 agccctccgg cggggtccac gattcgatgc gtgttccgcc ccaactggag tcaattaaac
    19861 cgatggttac cccgagcttt tggtgcaact cgcgcccaaa ataataggcg caggcggaga
    19921 atccacccca gccgccttcg gcgattgtct gcgggctgca tactttccaa gctccctcga
    19981 tatcgttctg cggctccggc ttccagcttt tgcccacttt caacagccgg atgcccggat
    20041 gatgagcagc ggcgatctcc gctttagcat ccttggagac ccccacgccc atttccatat
    20101 tcgactgccc cgagcatagc cacacttcgc cgatcatcac atcctcgcag cgaaccgagc
    20161 tggaaccgct tacgaggaga acgtgggggc cgccggcttt cagcgccggg agtttcgcct
    20221 tccattcgcc gcggtcgttg gctgtaacct gctggtggag gtttgcgagt gcgacccgga
    20281 cggtttcacc gggctgcgcc cagccccaga tcaacaccgg ctggtcctgt tgcagcacca
    20341 tgtggctgga gaaaacatgc ggcaggcgca cttccgcccg ggtggttccc ggcaaagcgc
    20401 ccgccatggc caaggccatc acagccaaac cgattgcgac actcaatcct gatctacgca
    20461 tagtcttttc cttcaaatac agctttctgg tttccgtgtc cggggaaacg cttcgtcggt
    20521 ccctggagcg ttggcagtat tctcttccgc atcagacccg tcaataacag gatgattccg
    20581 gggcagattt tcgacgtgcg ttgccaggcg cttgggggca cactctctcc cacgcatggc
    20641 tgtcattaaa cccttcgccg ccttacgacc gaaaccggaa ctggcgccgc gaatctgcga
    20701 gctgccgtac gatgtgatgt cctcggaaga ggcgcgccag atcgccgcgg gcaatccgct
    20761 gagtttcctg cacatcagca agccggagat agatttaccg cctgccaccg atccttgcgc
    20821 gccggaagtg tatgtccagg gccggaaaaa tttccggcgc ttgatcgccc agggcgcgct
    20881 tcggcaggat acccagccgt gtttttacct ttaccgccag gttatgggtg cccatagcca
    20941 ggcgggactg gtcgccgcgg caagttgcgc agattatcgc gaaggccgca ttaagaagca
    21001 tgagttgacc cggcccgaca aggaagacga ccgtctgcgc cacatcgagg cgctcgaatc
    21061 ccagacgggt ccggtgttcc tggtctatcg cgcgacgccc ggtctggacg cgcttgtcgc
    21121 ccggcaaacc actgcggctc ctgatgtgga cttcatggcc cccgacggcg tgcggcatac
    21181 cgcctgggtc atcgccgagg cggcggcgat tcagttcatt gaggctgagt ttgcccggat
    21241 gcccggcctt tacatcgccg acggccacca tcgctgcgct gccgccgcgc gggtctgcca
    21301 ggcgcgcaac ggtgcgggcg ggagcgcccg cttcctcagt gtcctcttcc cgcacaacca
    21361 gatgcagatt ctaccctaca accgcgtgtt aaaggatttg aacggccttt ctcccgcgca
    21421 gttgcgggaa aagctcgctt ccgtgttcac tttccagcac cccggagttg ccaccccggc
    21481 gcgcaagcat acgctggggt tttaccttga gggcgagtgg cacggactac acttcctgcc
    21541 gccgtacacg gctgtcgccg acccgatcga gcggctcgac gtcacgctcc tgcagaagca
    21601 ggtgttagcg ccgattttcg acatccacga cccacgcacc agtgagcgca ttaatttcgt
    21661 cggcggcatt cgtggcaccg cagaactgga gaaactggtt aaccgtggtg agtacgcctg
    21721 cgccttttcg ctgttcccga ccagcatcga ggacctgatg acgatagccg acgccggagg
    21781 catcatgccg ccgaagagca cctggttcga gcccaagctc cgcgatgcca tgttctgcca
    21841 catgatttga cgccgcccga ccctttctct ccgagagccg ccgcaccctg ttgcctggcg
    21901 gtctccgcgc cccgatgaac acaacccggt cgttccaacg tctcctgcaa ggcccggcgc
    21961 ccctgctcgc gctcgcgccg atgcaggata tcacggacct gccgtttctg cggcttatcg
    22021 ccggctacgg cggggcggat gtctatttca ccgagtattt ccgagtctat gccacttcgc
    22081 ggctgaataa gcacatcctc aaatccatta ccgagaaccc cacgggcagg ccggtcattc
    22141 cacaaataat tggcagtgac atcccggcgc tcgtgcgcac cgcgcgcgag ttgcagcaat
    22201 acccggtccc ggcggtggat ttgaacctgg gctgcccggc gccggtggtg tgccgcaagg
    22261 gcgccggcgg cgctctgctc cgcgacccga agcgcgtgga tgctatcctc ggcgcgctgc
    22321 gcgaggcggt gcgcgtcccg ttcaccgtca agacccgcat tggcttcgac gcggcggagg
    22381 tcttcgatga tctgttgccc attttcgccc ggcatgcgct cgatctggtc accgtgcatg
    22441 cgcgaaccgt caaggagatg tatcggacgg aggtgcatta tgatttcatc gcgcgcgcgg
    22501 tggccgggct gccttgcccc gtgctggcca atggcaacgt gtattcctcg ccgaaggccg
    22561 ccacggtatt gcagcttacc ggcgcgcgcg ggctgatgat cgggcgcggc gtcatccgca
    22621 acccctggct gttccggcaa atccgtgagc atcagcgcgg cgaaacggtc tttatccccc
    22681 gcgggactga cgtgctggcc tacgtccgcg ccctttacca ggcggtttgc tcccctgcga
    22741 cgcgcgagtc tgcgcaggtg cagaagatga aaaagtatct caattacctt ggcgtaggcg
    22801 tggagccgac cggccagttt ctgcaccagg cccgccgcgt gacgaccacc gccgccctct
    22861 ttgatgtgtg cgtcaagttt ctggaccacg accggcccat gccgctggag ccgttcccgc
    22921 tggcgctcaa ggcgggggat ctcatggccg gcgagcagcg gtgagtctcc cggctttccg
    22981 gttttgtcat ccgtagcggt gcggccgctc catcgcgaag gcgtttggca ggtgccgcac
    23041 ttcccgcact ttgccatccc gcagcagcac ttccacgatg tcgaagcgca gcttcaccgg
    23101 cggattcctc aaccgccgca ggtaatccag cgcagcgcgc gtgagccgtc cccgccgttc
    23161 ccggttcacg gccgccgccg gccgcgccca ttcctcggaa gaacgggtct tgacctcaat
    23221 gaagaccaag caatcccggt cgcggaagat caggtcaatt tcgccccgcg gcgatcggaa
    23281 gttggcggtg aggaacttca ggccctggcg gcgcagttgt tttttcgccg cgcgctcgcc
    23341 ccgctcgcca tggcgcaagt gcagcggctt ttcatgcccg cgccaccagg cgcggatgcg
    23401 ggccggtaga ttcatgggta gccggaacag gattgctttg cgtgcagccc ggcctctttc
    23461 tataactgcg gggtgtggaa ctcaaatcaa atctccccat gaccgccgcc tccgccgccc
    23521 gcgcctgccc tgtgtgtggg caggatgatg ccacggtctg gctgcaaaaa aatgcgctcc
    23581 gtctggtgcg ctgccgccga tgcgcgatga tctatgccaa tcccgtgccg acggcgttgg
    23641 cctcgggcca atactacgat caggggggcg cggattacta tctctcgccg gccaagctgg
    23701 agagcgacta cgcgccggtg cgttatgaac gcgaactgcg gctgttccgc cggcattgtc
    23761 ggggcggggc ggtgctggac gtcggctgct cgtcgggcgc attcctgttc cagttggggc
    23821 ggcgctttcc gggcgattac cagggcctcg gaacggacgt aagcggtccg gccctggact
    23881 acgcagagca gcgcggcgtg gcggtccgcc gcggcagctt cctggaactg gactttggtg
    23941 gccgattgtt tgatgccgtg accttctggg cggtgctgga gcacctgctc gaaccggcgc
    24001 ggttcctcgc gcaagccgcg tcattgctca agccggatgg cctttgcttc gtgctggtgc
    24061 ccaatatgaa atcgctcgcg gcccgcctgc tgggcgcgcg ctaccgctac ctctacccgc
    24121 agcatttgaa ctatttcacg aaggcgacac tgatgaaact cgtggcgccg tgcttctcag
    24181 tcatcacctt ccacaccact cacttcaatc cgatcatcgt gtggcaggac tggcgcggcg
    24241 gcgggcgcga cgtctcccct cgcgagcgcg cccaactttt gcagcgcacc accgccgcca
    24301 aacaacatcc gctgctgcgc ccgatgaagg cgctttacgg attgacggag aacgccctgg
    24361 cggcgctgac cctagcggac aacctagccg ttgtccttcg acggcgcgag gcccgggagg
    24421 ccgaatcctc tcctcaaccg gattggtttg cgtcaaccgg ccgtgagggt tagttcctcc
    24481 gcgagttgtt ccagccgtcg gacagcctcg gggccgaagg actgatctag atcggcaaat
    24541 tgccggctta actctgcctg gtcggcggga gtcagaaagc ggtcggccat agggaaaacc
    24601 atttcatcct ccttccagag atggtcgcga tagagcgcca ccaggccgcg taggatttcg
    24661 cggagcgcgg gcagcgcgtc gcgtgggtct tgtccgggag cgggcgacca ctccgccagc
    24721 gcgctgacca aggcacggcc tttctcgtgt tcgtgggtaa gtccgccaat cggacagccc
    24781 tgagacggca cgccgcgctc gacgagccgg gggaagaaca gggcttcctc cttgccgtgg
    24841 tgcagttggt ccgcataccc ccggaggaat tccaccgcac cggcgattgt cccgggcgcg
    24901 gggagttgtc cgttgtccac gcccgcggcg atgacctcga gggcgttcac caccgctgcg
    24961 atgcggtcgt gttcggcttt taatatctgg caggcggtca tgtttggcct tttccgccgc
    25021 gcggtttgtt tagggcgccg gtgcgatacg atagaagcgc tgcggataat tcgtcgccgc
    25081 gtcgtccacg atttccaccg tggcgcccgt gttcgtcacg aagcgggcaa agctccaatc
    25141 gaacaggttg gtcgaggtat aaagcgcata agcgcctggg tcacccgtta aaaagagctg
    25201 gaagcggcca tcggccagcc agttcggttg cgtcagcagc ggcggcgtga gcggggttac
    25261 cgtcagcacc gcgttcaagc tggtaacagt gcccaccgca ttggtgacga gcacggaata
    25321 attgcccgca tcgctcggcg cgaggttcat gcgcgtatag ctgctggcgg tggcgccggc
    25381 gatgttggtc ccgttctggc gccattgata cgcgagcggg gcggtgcccc cggcgtcaac
    25441 gccaaacgtg gcctggccgc cctgcaccac ggtcaggccc tgcggctgcg ctgtgagata
    25501 cggcgggatg ttggtcacga agcgttccac gcgcacatta tcgaacatgg caaagctcag
    25561 atttgtactg tcggaaagcg aatcaaagaa atcccacagc ccgatgaaga tattgctgga
    25621 ggtgaagctg gccccgggga tcgtcgcgat cttcaagcca tcaatagacc agtccaccgt
    25681 cgtgccggtc ttgctgatga ttacatcgcg ccaggcaaaa ccaatggtgc ccacagccag
    25741 cgctccgctc tgcgcctgca cggcgggcgc ggtctggccg cccgggaagg tggcggcata
    25801 gtagctgttt ccgttgccgc gcgcattgct cgccgtgccg gcggcataaa cgcccgaggc
    25861 cgcgctatgg tgggtcgtgc cggtgaacgc gccgaagtcg ttaatggcgg tcgtcgaggt
    25921 atcagaggcc tgaccctcgc cgtcggcggc aaaccagtag ccgtcagccg tcgaaccggc
    25981 gccggtccat tgcacccggt tgccggcggt gccaatgccc gccgtgccgt gctgggtcga
    26041 gccgttgccc ccggcgggga acggaccgtt ctggttcatc cagagatcaa agcgcagttg
    26101 gtaatcgccg ctgaaactct ggccgacagg cgagatgttg attgcggcgg cgacgcccaa
    26161 cgaaacgttg gcctcgaatt tcaccccccg ggtcgaaccg ttggcggaat tcggcgccgc
    26221 cgggatgcca tagggcgcat agttgtaatt gaacgtgacg cggcaatccg gggaggaacg
    26281 gttcaccacc cagttagcag cggtgttgga gtcgaagtcg tcgaggaaaa gcgtttgcag
    26341 cggcgggagc atgatttcga gcaaagctgc cggactggtc accgtgccaa acgcattgga
    26401 caccaccact tggtaatagc ccgaatgggc gggttgcgcg gagttgcggg tgaggctgct
    26461 ttgggtggcg cccgccaagt cggcgccgtt aaagcgccac tggtaagcca agggagccgt
    26521 gccggccgcc gcgacggaca aaaccgcgtc cgcgccttgt acgacaagct gccccgccgg
    26581 cggggtaagg ataatcggcg ccatattcgt cgccggcggc gcactgagga aattcaacac
    26641 gtccgccatg tattgattgc gccgcgtggc ggaggtgatc gtctcgaatg ggaacccgaa
    26701 caataccgtg cggccgccgc ccgctgagcc atcatactga accgccgccg cgccccccgc
    26761 cgcataattc atggccacgg ccgccccggt gccatacgga gtgatcacgt ccggtgtctg
    26821 cacccagtag atgcctttgg tcccatcatc cacggtgacg ctggcacggc ccgcaaagat
    26881 gccgccgggg gcggcgttgg cggtatagct ggccgagttg tcgctgctga acgtggcgtg
    26941 aagctgttgg cggagaaacg cgcggtccgc cgccgtcggc ccggaggggc ggtcaagatc
    27001 gaacgccacg tccgccccgg agacgaacaa gctgccgttg cccgccagga acgtggcaac
    27061 ccgggactgc tcgacactgc tgaaggtttc gctgttctcg gtctcattgc cacaagccca
    27121 gacgacgatg ggataatccg aaagaagcac ctggctggcg gagatggctt cgttttgaca
    27181 ggaatcaaac gccatgccgt aggcgctgat cgcctggccg tgcggcacga cgtaatcaaa
    27241 ggcgttgatt cgccgcggcc agacccgttc gatggatccg ctgttgcccg gaggccgata
    27301 gttcctggcc ccgacattca tgcgcgggtt ggtggtgcga tcaaaccggt cgaacgcgtt
    27361 caccaccagc acccgcgggg ctcccgccgt cgcgggcgtg cggcacccca cgacttccga
    27421 gggcagcgat tcaccgccgg cattcgcggc ggcgatgcgg aagtaataag cctggccggg
    27481 ggccagccct gacaccgtat agctggtgac attgccgacc gagatgggat tgccgaagcc
    27541 atagccattg gtggagctat agatgacgta gttggtgggc gactggctgc cgcccacgct
    27601 gggcgggata ccccattgca aggtgatgtt gccattggcc caccccagcg cgcgcgggtt
    27661 gaccggcggt tccggcagga acgccagcgg cccggagcca tacgtattca tatatttcac
    27721 cacggcatgc atcgccgcct tgccgaccgc ggcccgcgct ttcggatccc gcagcagcgc
    27781 ggaatccgtc gcactatcgt gaaacgccac ctcgatgatg gtggccggca tctcgtagtt
    27841 gaacaggcgg ccgtcaattt ccgagtagcc gccggtgtaa gtcacggtgg tccgattata
    27901 ccacgggtat tccagcggcg gcgatcccag cgccaccagg tcatcgttaa cctccttccc
    27961 gcagatctgt gccagcgcag cctggttggt cgtgggactg ccggtaatga gggccaccgt
    28021 tccacgcccg ccccccgcat tcgagtgaaa actgatatgg atgcggtcat gcacgcttcc
    28081 ggaagcctcc cggttcattt ccgccgatat ccggggcggg gtggaccagc tatcgctttc
    28141 atcgttggcg ccggaatcgt aaatctccgt tggcatgccc acgcccaggc ccgcctgcac
    28201 ccagtagcgg cagctttcct cctcccgcgg gtagcccgaa gtcccgcccc cgcgatcaat
    28261 gtcgcccatc ccgttcccaa aacgaatcgc gtcggcgata accacgctgc cgttcgccgt
    28321 gttccgcaag ttgctgacaa tgaccgcacc caccgccgca ttggacccgg cattgaagta
    28381 gtattcgccc aggtaaaccc agccgttgcc cacccggtaa tgcgggatgc gcacttgcga
    28441 ttcgccgccc gtatgccgga tccgatacaa ctggtcgccc cggttggccc cgtgcgacgc
    28501 ccagcaatac accggataat aacccgtcac cggaatggtc ggcgtatagg ttgccgttgc
    28561 ggtctccgtg ttatccaggt tcgcatagcg ataaggcacg tcgccaaccg tgccatagaa
    28621 ataactggtg gcagtgctgt tgaaccaggt gccgtcaaac ttcaccgccg catcatcgtt
    28681 gtccaacacc acttcattgg tctggtatcc cagcggccgg cagggaatca ccaccgcgcc
    28741 cgcgttgaaa cagtaagtgg caaacatgtt caactggtcc acgttcccat agtcctcatt
    28801 catatcgagc agcaccgggc gcagcaaaac ccaaccggac ttatacgtcc acccatgtcc
    28861 gccattcatg aaaaccagct tgccgctcag cgcgccggcc ggttgcgtcg ccgccggcgc
    28921 aatggggcca aaacccttgc tgggggcggt tgcctccgcg cccttgggcg ggacatctat
    28981 gcttgaccaa tccggcgaag gcggcagcgg agaaaggaat gactcatcca ccagccggtc
    29041 ttccaggccg gcgaaagcag tcaagccaac aaccacgcaa ctcacgaaaa gtgcactacg
    29101 aaatatacgg cgaagcatag ctatatcatg acatttttca gccagattac aagccgaaag
    29161 ttcggcaaat attcaccaaa atctgctgtc cggagcaggg ctgttgccgc ctcgccggcc
    29221 ggttttgccc ggcggtcagg cgaggggggc tggcctgcca ccgcttatga ggcttttgag
    29281 tttttactcc cttctaaagc gtttgtgctc cgagggactt gcaagcggac aggatgcccg
    29341 cgcgcctgcc atgccggtat aaaatgccca ggttaaacgt ccggggcgca atccggcgga
    29401 cggcgggccg gttccacggc agccgcaagg agcgttcgcc gcaaccagtc cagcgcctgt
    29461 tcggcggtga cttgcttgaa ggtctcgcgt tcgccgggat tgaagcggcg ctcgaccgca
    29521 acgccggtgg gggtggccag cccgatgaat accgtgccga caggcttttc cggcgtgccg
    29581 ccgcccggtc cggcgatgcc cgtgaccgac aaggcataat cggcgcgcgc gtggcggcgg
    29641 acgccgtcgg ccatttcccg cgccaccggc tcgctgaccg cgccgtggcg ggccaacgtg
    29701 tcggcgcgca cttcgaggaa tttctgtttg gcggcgttgc tgtaagtgac cagacccgcc
    29761 agaaagacag cggaagcccc gggcacgttc gtcacccggt gcgcaaggca gcccccggtg
    29821 caggattcgg cgagcgcgag ggtcgccttg cgctccgcga gcagccggac gacgaccgtt
    29881 tccaggtctt cgccttcgac gccatagatc cgggagccga gccgctctcg cacgatggtt
    29941 tccgccgacg ccacgcgggg ttccgcctcg cttccgcgtg cggcaaggcg cacatccacc
    30001 cggccggggc gggcgcaata ccccaggtcc aggccagcct cgaccaggcg ccgcagcggg
    30061 gcgtcaagct gctcctggac cgccgactcg ccaatgccgg tcgtccggag gatgcggcag
    30121 acaaactcgc cggacggcgg cagcacgcgc cgcagcaagg gcagcaccga ttcggcaaac
    30181 atcgggtgca attcaaccgg cgggcccggc agcatcacca gccagctcgc ccgcttcccg
    30241 gcgcgaaaag gattgggccg aacctccatc gccagccccg gcgcggtgcc gtgcgggttg
    30301 ggcaggacga ttgcgcccgc gggaaccagc gcctgtaccc ggttccgcgc gggcatttcc
    30361 cgccgccgac actcgaaaaa gtgccggatg tgcgccagga cggccgcgtc ctcgtgcagc
    30421 gctttgccca gcaactgcgc gatcaagtcc cgcgtcaggt catccgaagt cggccccagc
    30481 ccgccggtgg tgatcacgag gtcggcgcgc gccagcgctt cgccaaccgc ctggcggatg
    30541 tcggggccgg tatccggcac cgcgacctgg cggctaacgg tgtatccttg atccgtcagg
    30601 cggcggcaaa gccattgctg atgggtgttg agcacccgtc cgagcatcag ttcgctgccc
    30661 gtgttgatga gttcgacgat catcgcccgg agtgtaccgc cgggccctgg ccggccaaaa
    30721 ggcaaatgtt cggccgggcg caagcacggc ggcgcgaggc gcccgcctcc cagtccctgc
    30781 catcggccgg ccaacgaatc ccgggattca gagcgacaat aaatactcgc acaggtcgtt
    30841 aagctgatcc gcgctcaggt gcgaggtctt gccatgccgg tcggccgggt tggcggtggt
    30901 caatacgtcg cgcagcgtcc gggccgagcc gtcgtggaga tacggcgcgg tgcgccaaag
    30961 ctcgatgagc gacggggtgt cgaaggtgtc gtcggcttta tcgcaaggcc catgcgtgcc
    31021 gacgttgtag ctcttcagat tggtgaacca tggcccggaa tggcaatgcg cgcaggcggt
    31081 gtccgcgcgg cgaaagaccc gccgcccacg ccgggcggcg gccgagagtt tgcccgggcg
    31141 tccatgcggg ctgggaatgg gccggagcga ttggatgtag gcatccaccg ccgcgctgac
    31201 agccggcggc ggggtggcga acaggatatg cgtgaagccg gcgcggacgg ccgcggcggc
    31261 gctttcccgc acgccgaggc tcatggccgg gggcgtcgcg aaggcgccca gcaggctttt
    31321 ggtgtttttg gcgttgccga ggccgtcgtt gagcaggtcc cagttcaagc cgtccgtccg
    31381 cgcatcatcc gaatggcaac tggcgcagct ttgccagccc tgaaagcaaa ggcccgcatc
    31441 gttgaaataa agctccccct gctgcgcggc ggccagcgcc ggccgggggc cgagcgcgat
    31501 cgtggcagtc ttcggctgcg ggccgtccag ttcgaatacg ctgagggtat cggagaaata
    31561 attcgccgtg taggcggtct ggccaatcac ccgcaccccg cgcgggccca ggtcgccgtc
    31621 cggcaagggc cgccgctcgc gcaaaccggc aagcagggac aaatcgttgg gcacgtcgga
    31681 gggcgcctgc gagccaatgg tcgggtaggc gccgctaacg gttggttgga agacgggcgt
    31741 gagccgggcc agtttttccc gcagggccgg gaagtccgtc acgctaagct catgcgtgcc
    31801 ggccagcgcc acgaccagct tccggccgtc ggcgctccac gccgcgcccc agggattggc
    31861 cgcgccccgg tcctccgcat caagcagcac cgtgttcacc acctccaggc gttcaaggtc
    31921 aatgagggtc ttggcgttgg tgttcatcca gccgcgctcc agttgcaagg tcggcaggtg
    31981 gaaccgcgcc agaatatgtg tcgcaaccgc gtaacggcca tcgggcgaaa ggctcaggtc
    32041 gttgagcagg ccgctgccgc tgggcagcca aagctcatct acgacctggc cgcgcgcggg
    32101 gtctatgacg ctgatcaccg cgccggcgtg ctcgacatcc gcgcgcccgg cgtgcaggtg
    32161 attggccacc agcaagcgcc ggccgtccgg ggtaaggatg gccgccaccg gctcccgccg
    32221 gacggccacg cggcgaatct ctttcttcgc cgccaggtcg aatacgctca cgttgtggtt
    32281 gaagcggttg cagacaaaca gcgtccgccc atccggtgaa agcaccgggg cgcacgcgct
    32341 gtggccggcg ggcaagcgag ccgtaatccg gccccggggg atgtccacga tgcagacctg
    32401 gctttccggc gcggcgcagg tgacgaacag ggtttggcca tcggccgcga cagccaggcc
    32461 ggtcacgggc gccggcatcc gcgttgaacc ggtcacgcgc cgcgcggccg gatcgagcca
    32521 taacacgcgc cggccggcga cgcatcccac gaagagcgtg cgcccgtccg gcgtggccgc
    32581 cagcgtggaa ggcgcaagcc acgcgccgtc agccgcggca acgggagccg ccccgcagga
    32641 aaacagccac gcggccaaca gtccggcccg gtgggccgtg gagcgcaagc tgccggaagg
    32701 actcatcata tcgcaagggc acttctaacg gcccaaaccc gcaggggcaa gcccgaagcc
    32761 gggtttgtcc cggccatttt gccagccgct aaaacgcccg cccgggggcc gcgcccgccg
    32821 ccggaaagcc agcttttgac ttgggcgccc cagggagggc atactgccgc ctatggaacg
    32881 gactgacgcg gagttgatcg cggccgtgcg caagggcgac gccgccagct tcgagccatt
    32941 ggtccggaaa tactccgcgc ggctctttgg catggcccgc cgctacgcgc gcggcgagag
    33001 cgaggtcgaa gacctggtgc agaacatctg gctcaaagcg tttgccaatc tgcccagctt
    33061 ccgcggccag gcgcccttcg agcactggct gatgcggctg gcggtgcgcg tgtgctacga
    33121 ctcgctgcgc cgccaccaac gccaccgcga aacgctcttt gccgatctga ccgagccgga
    33181 ggcggactgg ctggagcagt tcgcccgcca gcccgattcc gccccggaag acgccgaggc
    33241 cgcgcgccag ctcgtcgcgc acctgctggc ggggctttcg cccgccgccc ggctggtcat
    33301 cacgctgctg gaactcgagc agcggtcggt gaaggaagtc gcgcaactga ccgggtggac
    33361 ggtgccggtg gttaaaatcc gcgccttccg ggcccgcgcc gcgatgcgcc gccagcttgc
    33421 ccgcgttgcc aaagaaaagt atttgtaacc gcgccccgcc ggcgggcgtc tgaatccttg
    33481 cagcctttat gaacctggac gaactggaac gaaaactgat ggccgccgcc cggtccgccc
    33541 cgccgaacga gcgggtgcct tgggcgtttg agaagcgcat catggcgcgg ctggccggcc
    33601 ggcccggggc ggatgaccgg gcgttgtggg cgcgggcctg gtggcgggcg gccgcggcgt
    33661 gcgcgggcgt catggtgctg ctgagcgcct ggacctggct cacgcccccg ggcggcgccc
    33721 cggcgaacga tttatcccgg gcgctggagc aaaccctgct ggcggccgcg gaactggaat
    33781 tccccggcga ttggaactga tgaacaactg gaaggtcatc ctggctacaa tggtcatctt
    33841 tggcaccggg gtggtgaccg gggggctgct ggtgcggcat gccgaacggg ggcgcattct
    33901 ccgaccgccg cgccccggcg ccgcggtccg tcccgcgccg ccggtctccg ccggcggaat
    33961 gcgcatcgag tttctgcggc gcctggaaca ggaattggac ctgacgcccg cgcagcgcga
    34021 gccgattaac cggattctca aggagggcca ggagcgcacc aagaagatca tggacaccgt
    34081 ggagccgcag cgccgggagg agttcagccg gaccattgaa gcgttccggg cggtgctgac
    34141 gccggcgcag cagcagcagc taaacgcatt gctcaaacag cagcagcggc gcgcgcgcga
    34201 gcagcgcaag gccgcggccc cgccgccgcg gccgcggccc ggcgccgcgc caaccccctg
    34261 agccggaccg ccgccgcccc gccccctaac gcgaccgcca ccggcgcagc cgcagggcgt
    34321 tgccgatgac gaccaggtcc gacatcccca tggccgccgc gcacagcacc gggctcagga
    34381 acccgagcgc ggccagcggc acggccagga tgttgtagaa gaatgcccaa aacagattct
    34441 gcttgatggt gcgcagcgtg gctcgcgcca gcccgaggct ttccggcacc gcctcgattt
    34501 ccgaccggag caggatgatg tccgccgcct cgcgcgcaat gtcgctggcg cggctgaccg
    34561 caatgcccag gtcggcctgc tccagcgccg gcgcgtcgtt gatgccatcc cccacaaaag
    34621 ccacccgctg gccttgccgc tgcagctttt gcaccagggc cgccttttgc tcggggcgga
    34681 cctgggcaaa gacgttcgcc gcggagatgc cggtctgccg ggcgatgccc gcggcggtca
    34741 gcgcattgtc gcccgtcacg agataaatct gcagcccctg ccgcgccagt tgccgcacca
    34801 cctcgcgggc gccgggcttg agcgcgtcct tcaccgcaaa caaaccccgc aaggtattcg
    34861 ccaccgccag ccccaccacc gttgcgccct gcgccgccca ttccgcgata aaatcctttc
    34921 ccggcgccaa atccaccccc gattcctcca gccagcgcaa cgcgcccagc cggaccgcca
    34981 ccggcgctcc ccgatccacg gcaagggtgg cggtcactcc gcttccgcga acctcctgcc
    35041 agcccgccag cggcgcgcgc gccgccgcca gcttcgccac cgcctggctg atcggatgcg
    35101 acgaatgttc cgccagcgcc gccgccagct cccgggcgga cgccgcggcg gctggcccgg
    35161 cgttcgaccc tcccggggcc gcctccgggt tcaggaggcg cccctcgtcc cacactttga
    35221 ccacttcggc ccggcccgtg gtgagcgtgc cggttttatc gaacagcacc gtgccgaccc
    35281 gtcccgcctt ttccagcgcc acgccatcgc ggatcaggat gccgcgttgc gccgcggcgt
    35341 tggagccggc catgatcgcc gccggggtgg ccagccccat cgcgcagggg caggcgacaa
    35401 tcagcacgcc ggcggcaatg atgaaggccg ccgccagcgc gccctccggc ggatgcgccg
    35461 cccagagcag cggcgccagc gaatcatgca cccgccgggc ccaatcaggc gccagccccc
    35521 agctcaaccc cgccgccagc gccactccca ccaccagcgg cacaaaaacg ctgctgaccc
    35581 ggtctcccag ccgctggatg ttggcgcggc tggcctgggc gcgctgcacc gccgcgataa
    35641 tgtgcgccag ggccgtctcc tcgcccgtcg ccgtcacgcg catcagcaac cgcccgctga
    35701 ggttgaccgt gcccgcatac agccggctgc cgggcgcctt gtccaccggc gccgattcgc
    35761 cggtcagcat ggattcgtcc agaaccgaac tcccctcctc cgccgcgccg tcggtcggca
    35821 cccggtcgcc ggggcgcagg acgacccggt cgccttcccg caactccgcc accggaactt
    35881 cgcgctccgc cccgtcgggg tcccgccgcc gcgccagcgc cggggccagg ttcagcaatt
    35941 ggcgcagggc gctcgacgcc cgcgcgctca cccgcgattc cagccagtgc ccgacgctga
    36001 ccaccgtgat gattgcggcc gcctccatga aataaacatg gccgcccgcg ccgcggagca
    36061 gcgcccacgc gctataaaca aaagccgtcg tcgagcccag cgccacgagc gtgtccatgt
    36121 tggagcggcc cgcccggagc tggctccacg cgccccggta gaacggcgcc ccggccacga
    36181 tctgcacccc gctggccagg gccagggaaa cccactggaa ccaccgcgtc agccccagcc
    36241 ccaggcccca ttcacccagc atgaggggcg ccgtgcccag aatccccacc cacaggttta
    36301 actgccaccg gccgggcgcg gccccggcgg cgggcgcgtc cgcgatacgg gcctcgaagc
    36361 ccgcctccgc gatcgcccgg atcaccgcgt ccacatcccg cgccgcgccg cccgcccagc
    36421 gaaccgccgc ccgcttcgcc tcgaggcgaa ccgtcgcgct gtggacgccc ggcacgcttt
    36481 ggatggcctc ggccacatgc cgggcgcagt tggcgcacgt catgcccgcg atggccaatt
    36541 ccgttcccgt ccgcggagcc gccggggcct cgcccgccgc gtcccgcgcc actgttggtt
    36601 caacttgcat agccgtatgc gttcaacatg gcccaggacg attctccgga caaccccggc
    36661 gtgatgttct ttaagaaccc cggcgggccg ggcgttttgg gttccggaac tccgcgggcg
    36721 gccggcggcg gaaagcgccc gggacttcac gccgcgcttg ccagccgccg gaagcgcgtt
    36781 aagctgcccg catgaagccg aatcatttgc tggtggcgct cctgctcgtg ctggcggggg
    36841 gcgtcatgcg ggtccacgcc caaccgaccg aggccgaacg aaaagacttt gccagcgtgc
    36901 aagcccgggc gaacaagggc gatgtcgaag cccaactcct cctcgccacc tactatacgc
    36961 agggaatcgg cgtcaccgca tccccgaaga aagccgtcca ggcgctccgc caggccgcgc
    37021 tccagggctc ggccatcggc cagtaccgcc tgggactgaa atatctcgac ggcgaaggcg
    37081 tgaaagccga caaggtggaa ggcgcccgct ggattcgccg ggcggccgat cagaagctgg
    37141 ccgaagcgca attcgttttg ggccaatgct acacctgggg cgagggagtc ggccgggatg
    37201 tcgttgaagc cgccaactgg tatcgcaaag ccgccgatca ggacctggca gacgcccaaa
    37261 ccgaactggg catctgctac ttcgaaggca acggcgtgcc caaagacatc cccgaggggg
    37321 tgaagtggac gcgccgggcc gccgaacagg gttttgcccg ggcccaaaac cggctcggtc
    37381 tttgttattc aaaaggcacc ggcgtgcccc aggattacca ggaggcctat atgtggctga
    37441 acctcgcagc cgcccagggc gatgcccggg cggaggatgc caaaatcaac ctggccgccg
    37501 cggaacgctt cctctcgccc gagcagatcg ccgaaagcca gcgccaggcg cgcgagtttc
    37561 gtccccgcaa ggccggatcg ccggcccctc cgccccccgc gcccgccccg acgaccgcgc
    37621 ccgccccgcc cccgcccgcg cctgaccgcc ccgccccggc cggcttcgtg aacgtcagcg
    37681 cccccgacga cgcctgtgaa atcttcgttg acggcgcctt cgtcggccac acgcccgccc
    37741 gggtgaaatt ggccgtcggc acgcacgagg tggaggtgaa gaagcccggc ttccggcctt
    37801 accggcggcc gctgcagatc accgaaggct cggaactcac cctgcgcgcc gtgctggaaa
    37861 agcaataagg cctcgcggcg ggctccgttt tcgaggcccg catccggcag cctttcgcct
    37921 tgccagggcg ggcgcaagcg cccagactcc ggggggtggc ggcccccagg ctggcaaatt
    37981 gggccgccgc gcctttgcta tgaaataccg attgtgcttc acgctcctgc tgctgctggt
    38041 attcaccccc acgccgatgc tggcgcaact ggccgcattt acctaccagg gacagttgct
    38101 ggatgacggc gaaccggcca atggcagcta cgacctgcgg tttacgctcg ccgacgcgcc
    38161 cgcgcagggc catctcatcg ccggcccact caccaacgcc gcggtagcgg ttcgcgacgg
    38221 tctcttcctc gtcacgctgg actttggcgg ctccgccttc gacggctcgc cccggtggct
    38281 ggagatagag gtgcggccgg ccggccccag cgcccccttc attcggctaa gcccgcgaca
    38341 gccgattacg gcaacgccct acgcgacgct ggccgccagc ctgctcccgg gagcggcggt
    38401 tgcgctcgac ggcgcgggaa tcaccaatct cgacgcctcg gctttggcgc ggggcaccgt
    38461 gcccctggcg cgtttgcgcg gcatcaccgc cacggaactg gatgcccata cctggcagtt
    38521 ggcaacccag ggaacggccg ggctgagttt caccccgcag tttggccatg cgaacctgac
    38581 gaactgggcc aaactcgaga cgaacgtgct ggccggtttg gcgggcgcgg gcaccagcct
    38641 ggcggcgggg acgaactggc tgcgcgcgat cagcaacgcg gttccggggg tttacaccaa
    38701 cagcggcaac ctctgggtga gcggctcgac cctccccgcg gccaacggcc tttacacgct
    38761 ggcggcgggc gcggtcttcg agctttgcac caataccgtt gtgtggacga accaaaacca
    38821 ctatggcgcg ttcgctcccg ccgttcgcga cgtggatatt tggagcaacc aacccctgac
    38881 catcacgaac ccctccggcg ccgccgtgct gtccaacgcg agtttcgtat cgtccgggga
    38941 cgtggtttac tacgtgccag gctatcccgc gggcattttc ctgaccaata atggcgtggc
    39001 ggacggagtg ttgcactggg ccagcaatac actcgtggaa ccgggctggc acgtggcggg
    39061 ctggcaggcg tccgggccgc tgcagttgga gggcggccgc tatgtcggag acgccagcgg
    39121 cctgtcgaat atgcctcccg tcgtccaggc aaagctcaac gacaccctgc gcgtttccga
    39181 gggcggggtg tttcgccgga tatttcacgc gcccccgctg ggaatctgca cctggcccgc
    39241 gttttcccgc cagattaatg acgaggtcgt gcgcggcctg gccgacagtt tatacacgaa
    39301 ccacctggtc gagcttggct ggcggctgct gcaactggac ggcggctggc agggcggcac
    39361 caacaactcc ggccgggaca ccaacggctt gctcacttac catcccctca acttccccga
    39421 ctgggacggc acccggcgct acctgcgcga gcgcggcttg acgctcggcc tgtatcacga
    39481 aatgaccaag ggaaatgaag gaatgcgcct gaccaatcat tacgtcgcgg atgcggcggc
    39541 cctgcactcc tggggagcgg actatgtgaa gattgacgaa ggaacgcgca cgctggcggg
    39601 acggttcagc gagttcgcgg cgctgcgggc ggcgggcgat gccatcggct ggaaccctta
    39661 cctgatgaac ggctcctgga cgaccaaccc cgcgccggat gcgcggttcc ccctggtcat
    39721 agatgccgtc cggctggcct atggcgacct ttacatcggg ggcaaccccg cctccacctt
    39781 taatgcgctg atggcgaact tccggcgcgg gatgcgatac ctttccgcgt tcgacaacca
    39841 gccgggcaaa tggaaggact tcgacttcct taccttgtcg cgggtgacgg gcgagcacag
    39901 cctccggatc cggcaaaccg cgctgagctt atggagcatt gcgccctcgg cgattctgct
    39961 ggacctggtc acgcccgata tgctcccctt gctgaccaac cgggccttcc tggccgtgca
    40021 tcaagacccc ctttgcgcgc ccggacggca ggtcgccagc aatgccacct gcgaggtctg
    40081 gatgcgcccg ctcgtttccg gagccaaagc cctctgcgtg ttcaacaaaa cggccagccc
    40141 gcaaaccaat atcgtcacgt tttcctccct gggcctgacc cccggccccg actgctatgc
    40201 cagggtgtat agcgtgtggg ataaccagac cttgtactac gcgaccaacg atttctccat
    40261 caacctcacc aactactacg ccaacctgct tgtggtctcg cccaaccggc tggccgcggc
    40321 ggcctcgttc ctgcccacga ccaatctgcc cctgcccggc gcaacctgga cgctccgatc
    40381 agatgggacc cacctctact ggagcagccc gtgagcaccc ggcgcgccct ggccggcggc
    40441 ctttccgtca tccgcgcgaa accgcggggg cattaccact tctcttggcg gacgtaatcg
    40501 gcgttaaagc gggtgcgcgg gggcggagct tcgccgggct ggccgagcga aatggcggcc
    40561 acgggaacaa acgaagccgg cagcgagagc aactccttga cgcgctgaat caggggctca
    40621 acggggtgga tgcccaccca gcaggcgccc aggccgtgcg cgtgcgccgc cagcagcagg
    40681 ttctcgatgg ccaccgagca atcctgcagc aggtagctaa gctggcgctc gaacgcaacg
    40741 tccagatcgc cgcagaccac tatggccatc gccgccgccg ataacatggc cgctccggga
    40801 tgcagcgtgg ccagtttcgc cagcatctgc cggtcccgga ccaccacaaa ccgccacgga
    40861 tctttcgtca tcgccgaagg cgcggccatc gccgcctcca gcagccgggt caccacctcg
    40921 tcgtccacct ccccggggga gtaaatacga atgctgcggc ggcccaggat aaagtccaac
    40981 atttgagatc tcggggtggt cataattgtc gcattaaata aaactccccc tgtctaacgg
    41041 gtttgcccgc cgttttcaac ccggaattgc cccgacctcg ccccggtctc ccgcccccgc
    41101 cgggcacccg ggccgggatt caatcccgca acctcctcct tgcgccagcc tctcggcagc
    41161 cccattcctg cctccttctg agccgtgtac aaggggtgta caagggatgc tcaccgggtg
    41221 tacataggat tttgcatagt acatccggtg agcatccgtt gtactcccgt tgtacatccc
    41281 ttgtacacag ctaagcgata gctatggaat agcttggaat gacgaaggtt aggtcgaaaa
    41341 tggcggttgg aatggtttaa tgggcttgga agtgaaaact gtccaataat tggacagttt
    41401 tctggaggtt ggagtgggtt ttaatcgtct gatttggtcc cgaacgatgg gcaggcgggg
    41461 aaagaggggg ccggggttga ggtgctattg gattacggcc agtcctaacc gcagggggtc
    41521 agtcgagggg tggtgcaggt tcttccgccg ggcgtggtgg gaaggttccc gagccac
//
LOCUS       DAOPQG010000017        41355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1262_length_41355_cov_15.445061, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000017 DAOPQG010000000
VERSION     DAOPQG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 41355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41355
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1262_length_41355_cov_15.445061"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            669..3215
                     /locus_tag="PKI07_03250"
     CDS             669..3215
                     /locus_tag="PKI07_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOC50098.1"
                     /translation="MLFALLLFALGTTASPAQEFRAAWVDIFHDGMSSASEVDTLVST
                     LASGNYNAVIVQVLGYMDMIGTASHGAHWQSSILPWSPRVKANFDPLAYLCTQAHAAG
                     IEVHAWIGGSGGAMYRVSLAANGWPPKNNATLAAHPEWFIAPRANSEGNAVVAVDIYY
                     SLDMGSPDVQEYLASIVRELVTNYPIDGINWDDEINGDGYLAGFGYPAYSQANYARSG
                     LARYRLNTGATGTPSNTDPDWSNYRRRFKNELMARVQAEIQSIKSNPRQPVRHTTAAL
                     AYGAPPGNCNFATSTPYLYFCDWAGMLQNGWVDAVIPQTYSKSTFKSWADRISSCWVS
                     VRPVFMGMSFSGLSISDITSQISYLRDKGLTGYATYSYFTAPANWWSHAAANVNTSTS
                     GTPTMPWRNPATATEGMMWGRVKDGATGLYVDDATVTVAGRPPVKTDGNGYYVATLIP
                     ATAAGTAYEVTATKDGLGSHAIANATVLAGDVVRYDFTLVVPPASPVLVWGDNTFGQS
                     AAPELATNAIAVAAGAWHTLALRADGRVVAWGDNSSGQCGDGEPMTNALAIAAGGYHN
                     LAIQRDGAVVAWGANDYGQASVPPGLSDVIAVAAGTWHSVALRANGTVVVWGDDSFGQ
                     KSRQPAGLTQVVAVAAGGNHTLALKADGRVVAWGENTDAEGNVVGQSEVPWDLADVVA
                     IGAGGYHSLAVKADGRVVGWGDNSQGQVSVPAGLSNVVAVAGGGMHSVALCGDGSVVA
                     WGANWSGQSGIPAWLPAAVGISAGEWHTVGLLEGTLPEARLHCPARKGSRFRVLAQTM
                     NRKTYVLERLDSLGETNWVGVSTNAGNGALRMLTDPTATAPQRFYRMKQW"
     gene            3223..3864
                     /locus_tag="PKI07_03255"
     CDS             3223..3864
                     /locus_tag="PKI07_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50099.1"
                     /translation="MTEWNIQARAHACAACSRPFVDQEGYHTLLFDEKADFRRLDVCQ
                     ACWLKQYSEGARERKGFVSYWQGVYTAPPPPVETIHKQTAESLLRQLIELNDPQYIPA
                     GYILAVMLERKRLLKVKEQILREGQRVFIYEQPATGDVFTIADPNLKLDQLEQVQRDV
                     AALLEHGLPPAPPVCPAAAGTDAAVVFPAPAAESAASSTPAPAEPADPVRMSG"
     gene            3861..4586
                     /gene="rnc"
                     /locus_tag="PKI07_03260"
     CDS             3861..4586
                     /gene="rnc"
                     /locus_tag="PKI07_03260"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOC50100.1"
                     /translation="MKDTIPAATGHQGLQERLNYWFGNPALLQLALTHPSVAHEHGAS
                     MQTNQRLEFLGDAVLQLVLTRELYEKFPAFDEGPLTKARAQLVNRRTLAERARQLNLG
                     QYLIVSRGEDLHGGRERPSALADTYEALLGAILLDGGYDAARNFILRQFQAAFGELSA
                     IPELENPKGELQELLQATSTEAPRYCVASASGPDHDRLFECTVHHGGKELARGRGKSK
                     KAAESEAALAALAKLRAAPESKP"
     gene            complement(4645..6015)
                     /locus_tag="PKI07_03265"
     CDS             complement(4645..6015)
                     /locus_tag="PKI07_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50101.1"
                     /translation="MPLALGLGLSLFLGFWADGSLVAAPLRLATFRCDTTPWKGEPLV
                     WTARLVKAETPLLAKGLILAEGTNRFVLCAFDWCLMGNDSDLLFRKALATAVGTDPAR
                     VAIQCIHQHAAPYADEGAHQLLDAAPQPPPHLSHEFLDSLRQQLAAAARAAADRLEPF
                     NRVGTGQARVERVASARRLKDENGQPITRYSNGAKSPQMAQAPEGLIDPWLKTITFAR
                     NDKPLVRLHYYATHPQTFCCDGRASADFVGLAREAVEQQENVFQIYFTGCSGDVTAGK
                     YNDGSTQAQADLRQRLQAGIEAAIAQTRFTRAKPIVWRALPLTLPPRTDQSQVAAQSR
                     AWLDNPAEYDGRRVYSGAMRLAYFERLNRPIQVSSLQMGKIHILHLPGEPMLDFQFYA
                     QQAKPRAFVAVAGYGDAGPAYICTDQAIAEGGYEPSATNVGPGSEARLKNAIQGLLGL
                     HFSPHP"
     gene            complement(6134..6868)
                     /locus_tag="PKI07_03270"
     CDS             complement(6134..6868)
                     /locus_tag="PKI07_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOC50102.1"
                     /translation="MPHRTFGPDEAARYLHLTPADLNRLVKEKEIPFERYGDRVVFRK
                     VEIDAWASQRILGMEGPPLAEYHQKSSSGARQIIAQDAIMPEMIRPEFVEPALTAKTK
                     GSVLRQMAALAEKTGLVCDPESLLQGLEAREELCSTGILGGLALLHSRNPESYLFEAA
                     FLALGRTIQQIPFGSPDGRATDLFFLIGCPDERMHLHTLARLCLMAQKTDLLLELRQA
                     PDAEAICDCILNCEQTVLASRKSASG"
     gene            complement(6929..7438)
                     /locus_tag="PKI07_03275"
     CDS             complement(6929..7438)
                     /locus_tag="PKI07_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOC50103.1"
                     /translation="MKLSRKIEKALNSQLNLELSSAYAYLGMAAYFESTPFTGFAKWM
                     QAQSAEEIDHAKRFFKYIVERGGKVDLQAIPASPCDYPSPLAAFKAALGHEQKVSASI
                     CAIFELAAAEKDYPTQSFLKWFLDEQVEEEQNVGEMLAKLELVGDHTMGIVHLDHQAG
                     RRGGEDQKD"
     gene            7809..8096
                     /locus_tag="PKI07_03280"
     CDS             7809..8096
                     /locus_tag="PKI07_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOC50104.1"
                     /translation="MNRLDRKRMQVRYSGQVQGVGFRYTARSVAAGYDVTGFVRNLPD
                     GGVELVAEGSKDELEAFREAIREAGLGHFIRHEDIAWQEAGNAFRGFEIVR"
     gene            8104..8826
                     /locus_tag="PKI07_03285"
     CDS             8104..8826
                     /locus_tag="PKI07_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOC50105.1"
                     /translation="MKFAIIADIHANLEALQVVLEDIKEQKCTHYACLGDVVGYNANP
                     KECLDIIRAMKMPVVKGNHDEYCSTDGPLDGFNPAAAEAVNWTRKQLTADDRQWLRDL
                     KYVRMVTNFTIVHATLDGPQRWGYVFDKLAAAASFTYQNTAICFFGHTHVPVAFMRDS
                     LVRGGTYSKFKVEPGKKYFVNVSAVGQPRDNNPKCGYVVYDADEGTIEQRRLEYDIAT
                     AQRKILDAGLPPRLAERLALGR"
     gene            8905..9924
                     /locus_tag="PKI07_03290"
     CDS             8905..9924
                     /locus_tag="PKI07_03290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC50106.1"
                     /translation="MKILILKPSSLGDVVQALPVLRLLKRHRPDSEIYWWIDARLAPL
                     LADDPDLAGIVRFERQRWARPWHWGEMGRSVRWLRRQAFDWVVDLQGLLRSGAFAWLA
                     NGGLTVGLDEPREGGRGFYDLIARRPSPATHAVDWYRQVLPLLGVPAEAEFQWLPERP
                     GVAAAVRRKWPVEPARWILLQPGARWLNKRWPAESFAGLVRWLAAADPSLRFAILGSA
                     DDRALGQAIARAGERRCLDLTGQVSLPELVEWIRLSALLVTNDTGPMHIAAALGKPVV
                     ALFGPTDPCRTGPYRQLEHVLQLDLPCVPCLKPRCTYVRPLECLRALPPAVVGAAVQK
                     RLAGA"
     gene            10010..10918
                     /locus_tag="PKI07_03295"
     CDS             10010..10918
                     /locus_tag="PKI07_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50107.1"
                     /translation="MKALRLALCLLAAAVGPVAAQVTVEVIQEQDQFLPGEAMPTAVR
                     ITNRSARDLRLGAEKDWLVFSVQTRGVEVVPKLGEVPVAGEFILGSSKVATKYVDLAP
                     YFFINRTGRYIVTATVRIREWDREVTSPPKEFNVSDGAKLWEQEVGVPAAPDSAPAVP
                     EVRKFILQQASSLKTQPRLYVRLTDATGARTFRVIPVGPTVSFGRPEPQVDKFSNLHL
                     LHQEGARSFSYVVINPQGEIIARRTYDYGDKRPRLQPDMDGKIIVQGGVRRPTPKDVP
                     PPQPAPAAEEEPKPLRSPGELVPPNL"
     gene            complement(10972..11538)
                     /locus_tag="PKI07_03300"
     CDS             complement(10972..11538)
                     /locus_tag="PKI07_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOC50108.1"
                     /translation="MRLRSVKPGVFIERDGILNRVRIERRHQVSPLTLEDFQVNQEAA
                     PLLRQLKAAGLLLIATTNQPGLSRGDQSRRELDRMHDLLRRVFPLDDILVCPHEAADQ
                     CPCRKPKPGLLLEAAFKWQVDLDQSFVVSDKWPDAEMARAAGCTSLLLQSPWLGDARR
                     DFTLPSLEAIVAKILALRAQPSALAVQS"
     gene            complement(11709..12761)
                     /locus_tag="PKI07_03305"
     CDS             complement(11709..12761)
                     /locus_tag="PKI07_03305"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC50109.1"
                     /translation="MRTFFHWALALLVLPVAAAERKFDFSEVREGQVPPGFRSTVTGQ
                     GKPGDWRVIWDEVPPLLPALTPGARSAARKAVLAQLAQDPTDEHFPLLIYEEETMGDF
                     ELTTRFKTVKGAVEQMAGIAFRIQNETNYYVVRASSRGNNLRFYKVLNGERGPLVGPE
                     LPVPSEVWHELTIECKGNSIRCRLNGQELITATDRVNPFASGKIGFWTKSDAVSYFAD
                     TRLVYTPHEPPAQALVRAAVKKFPRLLGLQVYVRGEEPRTTRLVANQNPAEVGRAGGK
                     VEWDVVHQGSPYCGKERTSVSAVLPLRDRNGEPIAAVRVILKPSPGETEQHIYARALP
                     VIRELQKRVTALNDLQ"
     gene            13142..13744
                     /gene="thpR"
                     /locus_tag="PKI07_03310"
     CDS             13142..13744
                     /gene="thpR"
                     /locus_tag="PKI07_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOC50110.1"
                     /translation="MAASDPMRLFIAIAIPEPVKARLETAQTELRRALPGASVRWARR
                     EQLHLTLKFLGEVAAARVEPLAEALRNACQPFAPLRLRLEGVGGFPNLRRPRVLWAGV
                     PDEAGRLAQLQAAVDGATRDFSNEKKEPGFAGHVTLARLRDLNRTEADALAERADGMK
                     QQPFGQWTVDQIKLMRSELSPQGARHTPLAIFTLAAVDTR"
     gene            14321..16180
                     /locus_tag="PKI07_03315"
     CDS             14321..16180
                     /locus_tag="PKI07_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50111.1"
                     /translation="MSQTSNQRSDDFLKISDQFQLGVLTTESSHPVTANLSETAKGDV
                     AAGLGLLFEADGDNVRKYREFAESGRARQIADTVLRVVKGGGKVFFTGCGSTGRLSIQ
                     LVSIWRDFWQKQRARGLQSKPSPEEFEHRAFSVMAGGDFALIKAVEGFEDFTEFGKKQ
                     IGDLGISARDAVFAITEGGETSFVIGTAWKGVEVGAKVYFVYNNPDEILCKHVKRSRE
                     VIEEPRIEKINLTTGPMSITGSTRMQATTIQLCVMLTVLEMVLRDLMKELEPQGPCAL
                     DSNAVPAEFLRKLTEMHGNLRAPDFRAQLARIVSLEEGVYRAKHKNNYYADRFGIDVL
                     TDTTERSPTFCSPPFRKFDDTTATESWSFLFLPYADTPKAWERIIKRQPQCVEWTEAE
                     VRALVSEDKVARTYEIVRKISAAELMRFKVGLDGLAHRPLRPGDSAVAILSETEKAAL
                     LAEDGFYRRQLEAAKKAGARTGLIYVGRAETFPEIRDFAARWNPEGVLVLAAVPATDF
                     LLDGVTRAGLKMLLNALSTCTMVRLGRVMGNYMIWVVPSNLKLIDRSTRYIQRLTNLD
                     YRAANRLLFEVIEYVEPRMKADQAYPPVVGVSVMRVRHQLDNAAAEQRIMAEM"
     gene            16228..16788
                     /gene="frr"
                     /locus_tag="PKI07_03320"
     CDS             16228..16788
                     /gene="frr"
                     /locus_tag="PKI07_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOC50112.1"
                     /translation="MALDDFLLEADDKMGKTEQVIVKEFAGVRTGKASPALVENIMVE
                     VYGSQMRIRELAGITTPEPRTLSIQPWDAGSLHPIEKAILKSNLGLTPSIQGKVIRLF
                     FPELSEERRQEFVKIVRKMAEEGRVAIRHVRRDTMDQFKKHGHDSGITEDDVKHAEKE
                     LQKLTDDYIAKIDAHVASKEKEIMTV"
     gene            complement(16864..18297)
                     /gene="mnmE"
                     /locus_tag="PKI07_03325"
     CDS             complement(16864..18297)
                     /gene="mnmE"
                     /locus_tag="PKI07_03325"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOC50113.1"
                     /translation="MAALFISAADFQSRNCRAGGEMHDDTIAAIATPLGVGGLAVIRL
                     SGPQALAIADRCFRPAGEAAPRPSAAASHTLHFGHIVRDGQTVDEALLAVMRAPRTFT
                     REEVVEITCHGGLLPAQMVLATVLASGARLAAPGEFTLRAFLNGRIDLAQAEAVVDLI
                     HSRTELALRAANEQLAGRLSRRVAALRDPLREILAHLEAHIDFPEEDIAPDAREQLIA
                     RLQHCEAQMDELLRTAPEGQLLRRGIRVAIIGRPNVGKSSLLNQLLGHDRAIVSALPG
                     TTRDTIEETANLRGLPVVFIDTAGLREARDEVEAEGIRRSRRALEEAECFLHVFDASE
                     PLTAADRERLAAEAGKRRILVRNKMDLPVRLQLPTAAGAPVVDVCCLTGQGIEALKDA
                     IKGLVWSGEIQAGMLPVMINARHEDALNRSRAAAQRAGETLRAGAPLELAALDLRIAV
                     NALGEIVGQTTTEDLLDTIFSQFCIGK"
     gene            18281..19942
                     /locus_tag="PKI07_03330"
     CDS             18281..19942
                     /locus_tag="PKI07_03330"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOC50114.1"
                     /translation="MNSAAMMRHSHSRDERYLSQAGGSGRAARKTAPPAAARADAAPR
                     RLQVFVFSEPERAGGYGRNWDGRAVGIGGRGAVLGDRGSGYDLGVRALQAVAAAYDQR
                     GVWPSLGRRLLRALQLNEPGDLMDWARTAEREEIARLAAEVFAARSRRDKLAADILAD
                     AAGQLARNAAACARRLARPGVRIQFVPAGSVFRAHPSFAAQVGRAVRKLWPRALVASQ
                     VLESVPDAAAPVKRERSSGARGAQNAVRGKGNKNPRPEAGPERVRSRRPSPTEQRNPR
                     SRRLDTLSLQRAIRLMLAEEGRVPGKLQAEHRRMARLIQAVVRAFRCGGRLFYVGAGT
                     SGRLGVLDASECPPTFGAPPGQVQGIIAGGRPALWQSVEGAEDQAEAGARALAYRGLG
                     RRDVVVGLAASGTTPFVWGALDAARRRGATTALICFNPFLRIPRARRPDVVIAPNLGP
                     EVLTGSTRLKAGTATKQLLNILTTLAMVRLGKVRSNLMIDLHPANAKLRGRAVRIVRE
                     LTGADAAGAEAALRRAGWNIRKAIALPGAKPRTGVWGTCPPSTRP"
     gene            complement(19863..20489)
                     /locus_tag="PKI07_03335"
     CDS             complement(19863..20489)
                     /locus_tag="PKI07_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOC50115.1"
                     /translation="MIKVLLLIFEPQVTWDKIEQAQRGVLFILGFFLLPLLLLTAGIE
                     GYGLANWGRWQGEVARLKKISVGEAVVVESAQVLLALLVVFAGARLLKEVGETFRGEP
                     SYTQAFRTVAYGLGPLFLLRLVDLFPVPSPWTSWAIGIVLSLGVLYHGVPRVMKPDPS
                     YAWSLYFLSGLLLSLLTGVVRFITGAYWAGKFPKLQSAVSHLAAQLPF"
     gene            20645..20968
                     /locus_tag="PKI07_03340"
     CDS             20645..20968
                     /locus_tag="PKI07_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOC50116.1"
                     /translation="MKVNLGIWDKLTPVVIFLLFIAIIMLVGMWYLPRIQQNQRMRKE
                     ILRLETLIRKEEETLRQLRSSADALLHDTKTVERLARERLGYARPGEIIIRFEEPRSN
                     GVSRR"
     gene            complement(20965..21876)
                     /locus_tag="PKI07_03345"
     CDS             complement(20965..21876)
                     /locus_tag="PKI07_03345"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOC50117.1"
                     /translation="MKPNPARFGVWARILLLLLGGAAASLGQRHPAGAPAPLDPERGA
                     QEARALVAELLALKPERNSTNTGWVRIRDAAGKERQIPARFEVFLTPTHWVSAYETLP
                     APDSPGGVRLTITRAAQQPNRYELRDPADGGAPPPAARELTAAEIMQPFAGSDFWIAD
                     LGLEFLHWPRQQVLKYEMRHGKSCKVLESINPQPVPGGYSRVVAWFMVESPHGLIHAD
                     AYDAQGSLLKRFDPRSLEKVDGEYQLAEMEIRNRKTGSQTWIKFNLGREQENGATPER
                     SPPPSRPAQTPAAPHPPEHRPPPVPAA"
     gene            complement(21873..24269)
                     /gene="lon"
                     /locus_tag="PKI07_03350"
     CDS             complement(21873..24269)
                     /gene="lon"
                     /locus_tag="PKI07_03350"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOC50118.1"
                     /translation="MTSPDTEFISILGTAGNAETPERVSSRVLPEILPILGLSDIVVF
                     PGMVAPLLVETAQSIKLIDDVVGGNRLLGLALQRNAEAENPRPEEMHEVGCAARVLKM
                     LKFPDNTVRVLVEGLWRIRLKEYPATDPYLRARLELLRDQKEDSVELTAMMRNAQSQF
                     QEIIKLSPALSEQIKIAALNTEDPGHFADLIAVNLNLNLEERQKLLETNSVKERLTRL
                     LPMLNREHEVLTLSSKIQSDVATSISKTQRDFFLREQLRAIQRELGESDPNAGEIRLL
                     RERIEQTPMPEEARKAAAQELERLQQMPPAAAEYGVSRHYLDWILSLPWERETEDKID
                     LAEAERILNEQHYGLGKVKDRLLEFLAVIKRRKQIKGPILCLVGPPGVGKTSLGRSVA
                     DALGRKFARIALGGMRDEAEIRGHRRTYVGSLPGRIIQTLKRVESRNPVILLDELDKV
                     GADFRGDPAAALLEVLDPAQNHTFTDHYLDLPFDLSRVLFLTTANWLDPVHPALRDRL
                     EVIELPSYTESEKLQIARRYLVPRQLEEHGLARKEVRIPDATLRRVIHDYTREAGVRQ
                     LEREIAALARKAARRLVSDGASAKPLVMGPDTLKRYLGTARFPNETAETIKEAGIATG
                     LAWTPVGGEILFIEATRMPGKGNLLLTGSLGEVMKESAQTAISYLRSQAGALKLDLAD
                     YAQYDLHIHVPAGATPKDGPSAGVPLVAALASLLTRRRVRSDVAMTGEISLRGRVLRV
                     GGIKEKVLAAARFGLKQVILPEQNRGDWSEVPEEVRRKLQAHFIRHISELLPLALRAS
                     "
     gene            complement(24327..24743)
                     /locus_tag="PKI07_03355"
     CDS             complement(24327..24743)
                     /locus_tag="PKI07_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOC50119.1"
                     /translation="MAMCKVSSSVHFVTRSTAGSQAEPGRAHWVPNTDVYTTDSGLVV
                     KVELAGMRSEHLEITVEGNRLRIAGNRPDGCRAAHCSFLVMEISYGPFESLLEVPSDY
                     DLSQAKAAYLNGFLRIDVPLAQRPVKRSRVLVAEGD"
     gene            complement(24796..25002)
                     /locus_tag="PKI07_03360"
     CDS             complement(24796..25002)
                     /locus_tag="PKI07_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50120.1"
                     /translation="MDWQQLVSLAIVASAATLLLGSRFRRRKYCFERVSRCGCLERLS
                     SGPSGAIVFRARKGERPEVRLKLR"
     gene            25493..28417
                     /locus_tag="PKI07_03365"
     CDS             25493..28417
                     /locus_tag="PKI07_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC50121.1"
                     /translation="MSRKIASQWVCYLAVAALALGMQGHRASASQLLPPGFRPLPLGT
                     HALTGGRIVIRPGEVLTNGTLVIREGRIAAVGKDVAPPADARVWEMKGTTIYAGFIDL
                     YLPLEATNSAAASAPGEAAAGDGLTASGPRFFGVPGAQPDRGPAGPGYAVAGITPERQ
                     AVRSYAPKAAALAPMHALGFTAGLVAPARGIIRGTSALVALSGDAPNAVVIKPEVFQH
                     IGFTPMESAERVYPSSLMGVIAAVRQAFFDARYYALDQADYQQNPQARPRLEFNPALA
                     ALAPAADRKQLVAVEPGSVLMTDRAARIAHELGLDFCLIASGQEWRRPDLAQATGAAF
                     IVPLDFPELPSLPDEDDWEQVQLDSLRAWDWAPENAAVLRQRGLEIALTTWRLADKKK
                     FRPNLRRALDRGLAEVDALAALTTVPAKLCGVASQLGTLEPGKLAYLTVVEGGSYFDP
                     EAKVREVWIDGRVYPVPGEERGAAQAGASQSRTAAANKPKPAKVAADNPGERRRALQK
                     TRLARSPLEGRGPLAEPKAILIEQATVWTCGPEGRLEDADVLFVGGKVKAVGKGLSRL
                     SPDGDPPLVIDGRGRHVTPGLIDAHSHTAILGAVNESSLPSTAMVRIRDVVNSETANL
                     RRQLAGGVTLVNLFHGSANPIGGQSCIIKLRDGAGPEDLVFDAAPPGIKFALGENVKQ
                     SGWGDRFVTRFPQTRMGVRTLIANRFIAAREYLARREQASGRSALPPRRDLELEALGE
                     VLQGRRWIHCHAYRQDEILMLIRLMEGFGVRIGSFEHALEGYKVADEIARHGAAGSTF
                     SDWWAYKFEVYDAIPYNGSLMRDRGVLVSFNSDSSELARRLYTEAAKAVKYGGMPEVE
                     ALRLVTLNPAKHLHLDARVGSLEPGKDADFALWSHSPLDSRTVCLQTWIEGKKYFDRE
                     QEAGRAAQLQAERAALLARAQKLAKLASGGGATSEEAKNSFFRVALEHEFDGCDRREL
                     EE"
     gene            28423..29853
                     /locus_tag="PKI07_03370"
     CDS             28423..29853
                     /locus_tag="PKI07_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC50122.1"
                     /translation="MRKFISHFRFERAGLPATFNRRRFASPPGRARALTFPRRRGCLS
                     LCGAVWLAALTPGVAAESLLLSGAIVHTVAGETLAPGQVLIRGGKIAAVGQTVPADGA
                     AQVDLAGRYLYPGLIALNTALGLTEISAVRATQDSTEVGDDFTPDVESGIAVNPDSEL
                     IPVARANGITSFEPVPQGHLISGQSALMAADGWTTEQMTIRNPLALHLFWPSRELDFT
                     PKEKARNRAQWKSLADQDQKRRARLRAIADFFAEAGAYARAKDAAARGQAPAPEIIPA
                     WEAMLPYVRGERPVVVHADEIRQIKAALAWAETQRCRIILAGARDAWMAADQLAARKI
                     PVLYGHVHTLPARDTDPYDVHFRAPAILHQAGVRVAFTLGEGSFDAAMIRNLPYLAAQ
                     AAAFGLPEAEALKGLTLYPAQCAGVADRLGSLEAGKDATLFAADGDILDIRSRVQRMW
                     IAGREVSLESRHTRLYEKYKNRPRPE"
     gene            30128..30340
                     /locus_tag="PKI07_03375"
     CDS             30128..30340
                     /locus_tag="PKI07_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HOC50123.1"
                     /translation="MSELVFEVTQEEDGGFCAECLSANIFTQGDTWEALRKNVQEAVR
                     AYYFDQPPAPKRIRLHLVRDELLADA"
     gene            30337..30579
                     /locus_tag="PKI07_03380"
     CDS             30337..30579
                     /locus_tag="PKI07_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOC50124.1"
                     /translation="MKVPRDLSGAQLIKVLCRDWGYRQLHQEGSHVILQTEMPGHQRL
                     SVPNHNPLRVGTLNSIARAVAAHKGVDRQAVLDSLG"
     gene            30713..32344
                     /locus_tag="PKI07_03385"
     CDS             30713..32344
                     /locus_tag="PKI07_03385"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOC50125.1"
                     /translation="MSIDCDLLIRNGTLYDGSGGPPFRGDLAVNGQTIAAIGPLTAVR
                     GRVELDADGLAVAPGFINMLSWANRSLIEDGCSQSDIRQGVTLEVMGEGSSMGPLNPT
                     MKRQLRERQGDIKYDIEWTTLGEYLEHLVRRGVSCNVASFVGATTVRIHEIGYTDRPP
                     TPAELERMRQLVREAMAEGALGVASSLIYAPACYAGTDELIALCQVAAECDGLYISHL
                     RSESNRLLEAADELIRIARETPIRSEIYHLKVAGRPNWPKLEPLIRKIEAARAEGLAI
                     TADMYNYAAAGTGLDAAMPPWVQEGGLEAWVQRLRDPAVRQRVKREMAAPADTWENFY
                     VAAGSPERIVLAGFKSDRLKPLTGLTLAEVAARRGTSPEEAAMDLVAEDQGRVDAIYF
                     IMSEDNVRRQLKLPWVSFGSDEGSFAPEGVFLLSNPHPRAYGNVARLLGKYVRDEKLI
                     PLEAAIHRLSALPAANLKLDRRGALKPGFFADIVVFDPAAIQDHATFAQPQQYATGVR
                     EVFVNGVRVLKDGEHTGAKPGQVVRGPGWRRPAAG"
     gene            complement(32364..32993)
                     /locus_tag="PKI07_03390"
     CDS             complement(32364..32993)
                     /locus_tag="PKI07_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOC50126.1"
                     /translation="MFQLLFPQYRRRRWVYLPLGALALGEQRPGKPNPLLEPDYCQRW
                     VRRLHRRYRATCSYGGWFEDRSTLWQGHYMHPNMAFHLGVDFNVAERSRVYSPADALV
                     VETWHDEDSSGGWGGRIALELRPRLTLLLAHLGKTWVRVGDRVRAGQLLGNVGGPTNN
                     GNWFPHLHAQMVRGSFAHVDGYGAFSVSNQRKFPDPFRFWPPQYDYAHA"
     gene            complement(32995..33507)
                     /locus_tag="PKI07_03395"
     CDS             complement(32995..33507)
                     /locus_tag="PKI07_03395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOC50127.1"
                     /translation="MPRRPAIEIREMQVDDIPAVVELGNMLFTAETSPTLYRCWEEAE
                     VLQIYTADKETCLVATCHERVIGFALGSLLEKPGSSWLYGWLDWLGVDPAFKRKRVAK
                     RLTRQLQERFVEKGVRIMLVDTYEGNRNAIAFFRKFGFGQGIRHIYMSLNLDNHPKAI
                     ERKFMNDPGD"
     gene            complement(33570..35450)
                     /gene="cysC"
                     /locus_tag="PKI07_03400"
     CDS             complement(33570..35450)
                     /gene="cysC"
                     /locus_tag="PKI07_03400"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOC50128.1"
                     /translation="MNPDAAEQFRIVILGHVDHGKSTLVGRLLHDTGSLPEGRLEQLQ
                     LAARRRGVPFEWANLMDALQAERDQNISIDTAQIWFRTPRRRYVIIDVPGHHEFLKNM
                     VTGAAQAEAALMLIDAREGVRENTRRHGFLLEFLGLRQVIVLVNKMDLLNYAPEPFRA
                     LEAEYRAWLQALGLQPRLFLPISAWHGDNLAAPSPRMPWWRGPTVAEALDQFEAVPPP
                     ADQPLRFPVQDVYRFDERRILAGRIESGRLKVGDRLVFVPGYKTSTVRSLERWNGPAR
                     TVAEAGESIGLTLTEQIFVERGAVGAPEDAPPYALTRFRARVFWLGKQPFAPGRAYRL
                     KLATQEAACQIEHIERVIDAATLQPLERPGAPGVHRHEAAELTLRTRKPIAFDPRAEI
                     AALGRFVIVDGFDLAGGGVILDDRYPRRTADSLHKSPNIYWTVGRVTAAQRARAHGHA
                     GRVVWLTGLSGSGKTTLAIELERELFERGQWVYVLDGDNVRHGLCADLAFSPADRREN
                     IRRVGEVARLFADAGVICVTAFISPYRADRDAIRRSLPPGQFVEVYLNAPLAVCEQRD
                     AKGLYAKARAGEISDFTGVSAPYEPPEHPELELPTAELGVDECVARLRDFLAGLEEPA
                     AE"
     gene            complement(35451..36260)
                     /gene="cysD"
                     /locus_tag="PKI07_03405"
     CDS             complement(35451..36260)
                     /gene="cysD"
                     /locus_tag="PKI07_03405"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOC50129.1"
                     /translation="MLLDELENQSIYLLREAFSQFERLAMLWSIGKDSTVLLWLARKA
                     FFGHVPFPLVHVDTRYKIPSMIEYRDRLCREWKLQLIVGCNDAALAGGRTYPQGRATR
                     VECCSALKKDALQAVLHQHGFTGVIVGVRRDEEPTRAKERYFSPRDRQMEWNVEDQPP
                     EFWDQFQTDVAPGTHIRIHPLLHWTELNIWEYIEREKVPVIPLYFANERGERYRSIGC
                     YPCTFPVKSSARTVAEIVAELRQTRTPERAGRAQDQESEAAFEQLRRDGYM"
     gene            complement(36587..39682)
                     /locus_tag="PKI07_03410"
     CDS             complement(36587..39682)
                     /locus_tag="PKI07_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC50130.1"
                     /translation="MKPTSLKPLLTLILALLFLGAASLPALELSGRKVVIDPGHGGSD
                     PGATGYDGPGKPNEADFNLAVSLKLRTLLQAAGCSVVMTRSTDVAVDLYARRDLVNAE
                     SPHAGLCIHCNSFSDPGAHGTETFWCLDYCNGVAAEDQDLATKVQNRLIQFLGRANRG
                     VKRYNFVMCSPTPPSCLAEMLFVSNQAEFNLINSTAGQDNAAAAFFYAVCDRVGVTTP
                     AAPSGLTATAASASQINLSWNDNSGLEHSYKVERATASGGPWTEIATTAANATTYAST
                     GLSGGTTYYYRVRAYNSHLGNSAYSGTASATTQVSGAPSITSQPASRTVDPGSTVTFT
                     VVATGNPTLTYQWRKNAVNLSNGGKYSGATTATLTISNVQQTEVGNYSVVVTNGFGAT
                     VSANAALAVNAVIAFYDDFESPAINWTNFVPPATALTVSTAQSVSPTRSAYVNSSLDR
                     MYRNLGVSVDGRLRITAWIYDSTQTRSFVDVRGYTGGGYNQGSLVQLFCAGKYNTVTM
                     PGETYDATKYQGRVVSGANNGWFNLNAPGAPARSAGWHKFVIERRADGTTVDFYVDGI
                     LSRTITGTTRSSLDSAAIGSVGTGSEVLGDSWIDDVKVEYFDLPQITTQPASQTVAAG
                     GTATFFVETANTVTAYQWQKNGTNIAGATTSALVLSNVQDSDAGSYGVTVSNGAGPVV
                     SATATLSVAPAIVAQPLSQTNSAGATASFTVTAAGQLPLTYRWQKGGVDLADGGNISG
                     ATTPELTLNTVSQADAGSYTVAVSNAVGGVISMPAWLVVADPPDIVTPPASQAVAAGS
                     TVTFSVVANGTPPLSYQWRFNEIAIAGATDSSYSRPDVQSADAGNYSVEVSNAAGSTI
                     SPDATLAVNTAPVLSAINDLAVHAGATATVTASAYDPDAGQTLTYSLGAGAPAGATIG
                     AASGIFTWPTTDGDAGSNPAITIMATDDGLPSLSDSTTFSIAVAAPLRIQKVEWVGGR
                     VVLTWTAIAGRNYRVEYKDNITDLNWSNLPPDITASADTASKEDAVGAPQRFYRVLSL
                     D"
     gene            complement(39719..40753)
                     /locus_tag="PKI07_03415"
     CDS             complement(39719..40753)
                     /locus_tag="PKI07_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50131.1"
                     /translation="MTIKAAVMLLPASTTWRVAVLLKPCLALGLALGGGWTVAAAPPA
                     APAPAGRASAVQIARVELLSGERAGYVRPAWSPDGQWLGLGRAGMAGIELARRDGKEW
                     KLLTLETDSGYRFDWSPDSARIAYRTRRTESNLTWYVIKSVDLASGKTAELSKPAGEV
                     SPPVWLSSNGNQCVSFLLGTNLVNTPWQRAKRKVGTRASQPAPLLFTDRGQVWRAGPD
                     SGVRTALTTDGGMEPVWSPDRSQVVYSQMGVLMIMAPDGSKKRELARGHHPSWSPDGT
                     MLVYDVSRDDGHQILSSDLYVINVDGTEPTRLTHTPEVLECEPAWSPDGRCIACRTED
                     AGRVLLLWLQ"
BASE COUNT         7044 a        13497 c        13583 g         7231 t
ORIGIN      
        1 accttgagtg gaaaccatga cgaccaattt gctgaacttg acggacacaa cttcctctat
       61 catcgtttcg gcctttccaa gcaaccatag ccattgtcca aataccctga aaatcccctg
      121 ccagcaggcc gtgtacaagg ggtgtacaag ggatgcccat cgggtgtacc agctaaaatc
      181 caatgtactt ccggtgagca tcccttgtac accccttgta cacggcgtaa ggatagggta
      241 aatgcaaggc agattcatta tgtgctcagc cgggacggat tagattccgc cggcatttct
      301 ccacgaatta tcggggcaac gatccctcaa gctcccgctt ttcctggttt tcggtcccca
      361 tgccgtgatg cggcatggga atagcacgga atggcgggga aacggaggga cggtgaagag
      421 agcataccca aatgccgtgc aagcatctga ttgcgcatag gtgcggcagg ttgattcgag
      481 gtgattgcaa tcatgggcag ataagcctga cacggcgata ttgccgcatt gactcttcgc
      541 taaaatggcg cagtatattg cgatatttga ctgtctgaag ccctatgaat gcactccgct
      601 atcgccaacc cgcatgcttt gccattggaa catcctcgct ccggcgctgg agtatgttgc
      661 ggagaggctt gctgtttgca ttgctgcttt tcgccctggg cacgaccgca tcccccgcgc
      721 aggaatttcg ggcggcgtgg gtggatatct ttcatgacgg catgagcagc gcgagtgagg
      781 tggacacgct ggtttcgacc ctcgccagcg gcaactataa tgccgtcatt gtccaggtgc
      841 ttggttatat ggatatgatc gggaccgcct cgcacggggc ccattggcaa tcgagcattt
      901 tgccctggtc gccgcgcgtc aaggccaact tcgatccgct tgcttacctt tgcacccagg
      961 cgcatgcggc tggcattgaa gtccatgcct ggattggcgg cagtggcggc gccatgtacc
     1021 gtgtctcgct cgcggcgaac ggctggcccc caaagaataa tgctaccttg gccgcgcacc
     1081 cggagtggtt catcgctccc cgggccaaca gcgaaggcaa tgccgttgtg gccgttgaca
     1141 tctactacag cctcgatatg gggtctcccg atgttcagga atatctggcc agcatcgtga
     1201 gggagttagt gacgaactac ccgattgatg gcatcaactg ggacgacgaa atcaatggcg
     1261 acggctatct tgcgggtttt ggctacccgg cgtatagcca ggccaactat gcccgttccg
     1321 gcctggctcg ctatcgcctc aacaccgggg ccaccggcac tcccagcaac actgaccccg
     1381 actggtccaa ttatcgtcgc cgcttcaaga acgagttgat ggcccgcgtc caggcggaaa
     1441 tccaatccat caaaagcaat ccacgtcagc ccgtgcgtca caccacggcg gccctggcct
     1501 atggtgcgcc gccgggcaac tgcaatttcg ccacttccac tccctatctc tatttctgtg
     1561 attgggccgg gatgctgcaa aacggctggg tggacgcggt gatcccgcag acttatagca
     1621 agagcacttt caagtcctgg gcggatcgta tctcgtcctg ttgggtttcc gttcgcccgg
     1681 tgttcatggg catgagtttc agcggcttga gcatctccga tatcaccagc cagatcagtt
     1741 atcttcggga caaagggctc acggggtacg caacctactc ctattttact gcgccggcca
     1801 attggtggtc ccatgcggct gcaaacgtca atacgagcac cagcggcacc cccaccatgc
     1861 cgtggcgcaa tcctgccacc gccaccgaag gtatgatgtg gggacgggtc aaggacggcg
     1921 ccaccgggct ttatgtggac gacgcaaccg tgaccgtagc cggccggcca ccggtgaaga
     1981 cggacggcaa cggctactat gtcgcgacgc tcattcccgc gaccgctgcc ggtaccgcct
     2041 acgaggtcac cgcaaccaaa gatggcctgg gctcgcatgc catcgcaaat gccacggttt
     2101 tggcgggcga tgtggtccgt tatgatttta ccctggtcgt gcctccggcc tcgccggttt
     2161 tggtttgggg cgacaatact tttgggcaaa gcgcggcacc ggagttggcg acgaacgcca
     2221 tcgccgtggc ggcgggcgcc tggcatacgc tggccctgcg agccgatggc cgggtggtgg
     2281 cctggggaga taattcgagc ggtcagtgtg gggatggaga gccgatgacc aatgccctgg
     2341 ccattgccgc cgggggttat cataacctgg ccatacagag ggacggagcg gtggtggcgt
     2401 ggggggccaa cgattacggc caggccagcg tgccgccggg cctgagcgat gtgattgcgg
     2461 tggctgccgg gacgtggcac agcgtggcct tgcgggccaa cggcacggtg gtggtatggg
     2521 gagatgacag ttttgggcaa aagagccggc aaccggccgg gttgacgcag gtggtggcgg
     2581 tggcggcggg aggcaatcat accttggcct tgaaggcgga cgggcgggtg gtggcctggg
     2641 gagagaacac ggatgcggaa gggaacgtgg tggggcaatc ggaggtgcct tgggatttgg
     2701 cggatgtggt agcgattggg gctggggggt atcatagtct ggcggtaaag gccgacggcc
     2761 gggtggtggg gtggggggac aattcgcagg gtcaagtcag tgtgccggcg gggttgagca
     2821 acgtggtggc ggttgccggc gggggcatgc atagcgtggc gctttgcggg gatgggagtg
     2881 tggtggcgtg gggagcgaat tggagtggcc agagcgggat ccccgcgtgg ttgcccgcgg
     2941 cggtgggcat aagcgccggg gaatggcaca cggtggggtt gttggaaggc acgctgccgg
     3001 aagcgcggtt gcattgcccg gcgcggaaag ggagtcgttt tcgtgttttg gcgcagacga
     3061 tgaaccggaa gacctatgtt ttggagcgat tggatagtct tggggagacg aactgggtgg
     3121 gggtgagcac caacgcgggt aatggagcgc tgcggatgct gacggatccg acggccactg
     3181 cgccccagcg cttctatcgc atgaaacaat ggtagccgac gcatgaccga gtggaacatc
     3241 caagcccgcg ctcacgcatg cgccgcctgc agccgtccct tcgtggacca ggaaggctac
     3301 catacgctgc tgttcgatga gaaagcggat tttcgtcgcc tggatgtctg ccaggcgtgc
     3361 tggctgaagc aatacagtga gggagcgcgc gagcgcaaag gcttcgtttc gtattggcaa
     3421 ggcgtctata ccgcgcctcc gcctccggtc gaaacgattc ataaacagac ggctgagtca
     3481 ctgcttcgcc aactcatcga gttgaatgat ccgcagtaca ttccggcggg atatatcctg
     3541 gcggtcatgc tcgaacgcaa acgcctgctc aaggtcaagg agcaaatcct gcgcgagggg
     3601 cagcgggtgt tcatttatga gcaacccgcc accggcgatg tgttcaccat tgcggacccc
     3661 aacctgaaac tggatcaact tgaacaggtt cagcgcgacg tcgctgcgct tctcgaacat
     3721 ggtttgcccc cggcgccccc ggtttgcccg gctgccgccg gaacggatgc cgccgtcgtt
     3781 tttcccgccc cggccgctga gtccgccgcc tcttccaccc cagctcccgc ggaacccgct
     3841 gaccccgtgc ggatgtcggg atgaaagata caatacctgc cgccaccggg caccagggac
     3901 tgcaggaacg gctgaactat tggttcggca atcctgcgct attgcaactt gcgttgacgc
     3961 atccttccgt cgcgcatgag catggggcat ccatgcagac taaccagcgg ctcgagtttc
     4021 tgggtgatgc cgtcctgcaa ctggtgttga cgcgcgagct ttatgaaaaa ttccccgcct
     4081 tcgacgaggg gccgctgacc aaggcccggg ctcaactggt taatcgccgt actttagccg
     4141 agcgcgcccg gcaactgaac cttggccagt atctgatcgt cagccggggg gaagacctgc
     4201 atggcggccg cgagcggccc tctgccctgg ctgacaccta tgaagccctg ctcggcgcca
     4261 tcctgctcga tggcggctat gacgcggccc gcaattttat tctccgccag ttccaagccg
     4321 cctttggcga actttcggcg attccggaac tggaaaaccc caaaggtgaa ctgcaggaac
     4381 tgctgcaagc cacctccacg gaagcgccac ggtactgcgt agcctccgcc agtgggccgg
     4441 atcatgatcg cctctttgaa tgcacggtgc atcacggcgg aaaggaactg gcgcgcgggc
     4501 gtggcaagag caaaaaggcc gccgaaagcg aagccgcgct cgccgcgctg gcaaaattgc
     4561 gcgcggctcc ggagagcaag ccgtaaagac ggatttcgcc cgcgccgagg gtatcgcggg
     4621 cggcatttcc tccgccgctg cggattaagg atgaggggaa aaatgcaatc caagcaagcc
     4681 ctgaattgca tttttgagtc gagcctcaga gccggggccg acgttggtgg cggaaggttc
     4741 gtagccgcct tcagcgatgg cctgatcggt gcagatatag gcggggccgg cgtcgccgta
     4801 accggcgaca gccacaaagg cgcggggttt ggcctgttga gcgtagaatt ggaaatccag
     4861 catgggctcg ccgggtaaat gcagaatatg gatcttgccc atttgcagag agctgacctg
     4921 aatggggcga ttgagtcgct cgaagtacgc caggcgcatg gctccgctat agacccgccg
     4981 cccgtcgtac tccgccgggt tatccagcca ggcccggctt tgggccgcaa cttgagactg
     5041 atccgttcgc ggtggcaggg tcaatggcag agcgcgccag acgatcggct tggcccgggt
     5101 gaagcgcgtc tgggcgatgg cggcttcgat gcccgcctgt aggcgctggc ggaggtctgc
     5161 ctgcgcttga gttgatccgt cgttgtattt gccggccgtg acatccccgg agcagccggt
     5221 gaaatagatt tggaacacgt tctcttgctg ctcgacggct tcacgggcca atccaacaaa
     5281 gtcagccgag gcacggccgt cacagcaaaa cgtctgcgga tgggtggcat agtagtgcag
     5341 gcggacaagg ggcttatcgt ttcgggcgaa ggtgatggtc ttaagccatg gatctataag
     5401 cccctcgggg gcctgcgcca tttgcgggct tttagcgccg ttgctatacc gggtaatcgg
     5461 ctggccgttt tcgtccttga gccgccgagc cgaggcaacg cgctccaccc gggcttgccc
     5521 ggttcccacc cggttgaaag gttcgaggcg atccgctgcc gcgcgcgcgg ccgccgccag
     5581 ttgctggcgg agggaatcca ggaactcgtg gctcaggtgc ggcggcggct gcggagcggc
     5641 gtccagcaac tggtgggctc cttcatccgc atacggcgcg gcatgttgat gaatgcactg
     5701 gatggcaacg cgcgccggat ccgttcccac ggcagtggcc agggccttgc ggaacaacag
     5761 atccgaatca ttccccatca agcaccaatc aaacgcgcac agcacgaaac gattggtgcc
     5821 ctccgcgaga ataagcccct tggccagcag cggggtttcc gccttgacca gcctggccgt
     5881 ccaaacgagg ggttcgcctt tccaaggcgt ggtatcacaa cgaaacgtgg ccagccgcaa
     5941 cggcgctgcc accaagctcc catccgccca aaagcccagg aacaacgaca gtcccaaccc
     6001 caaggccaac ggcattttcc tgaaaacgca acccgatgat ttcatatcac aacagaagtt
     6061 tgtcagtctt tacggtaagc gaaccgaagc tttttatcga gctttctttg cccggccatg
     6121 ggcgtggcgt tgatcagccc gatgcagact tccggctggc caggaccgtt tgttcgcagt
     6181 tcaggatgca gtcgcagatg gcctcggcat ccggagcttg gcgcagttcg aggagtaaat
     6241 ccgtcttttg cgccattaga cacaagcggg ccagggtgtg caggtgcatt cgctcatccg
     6301 ggcagccaat caggaagaac aggtcggtgg cccgtccatc cggcgaccca aagggaatct
     6361 gctggatcgt ccggcccagc gccagaaagg cggcctcaaa taaatacgac tccggattgc
     6421 gtgaatgcag cagcgccaaa ccgccgagga taccggtcga gcatagctct tcgcgcgctt
     6481 ccagcccctg cagcagcgac tcggggtcgc agaccaaccc ggtcttctcg gctagcgcgg
     6541 ccatttgccg caagacggag cctttggttt tcgccgtcag ggctggctcg acgaattccg
     6601 gccgaatcat ctccggcatg atggcgtcct gcgcaatgat ttgccgggct ccactggatg
     6661 atttttgatg gtattcagcc aggggtgggc cttccatgcc caaaatgcgt tgcgaggccc
     6721 atgcatcaat ctctaccttg cgaaacacca cgcgatcccc gtatcgctcg aaggggattt
     6781 ccttttcttt gaccaaccga ttgagatcag ccggggtaag atgcaggtag cgtgcggctt
     6841 catcaggacc aaaggttcgg tggggcatga agcaataaac aggtctgcgc tcggccgagt
     6901 tgactggcca acccggctcg gtccatgact agtccttttg gtcctccccg ccacggcgac
     6961 cggcctggtg gtcaaggtgg acgatgccca tggtgtggtc tcccaccaac tccaacttgg
     7021 ccagcatttc gcccacgttt tgctcctcct caacttgctc atcaaggaac cacttgagga
     7081 acgactgcgt cggataatct ttttccgccg cggcgagttc aaaaatggca caaatcgaag
     7141 ccgagacctt ctgctcgtgg cccaaggccg ccttgaaagc agccaagggt gatgggtaat
     7201 cgcaggggga cgcggggatg gcctgcaagt ccaccttgcc gccgcgctcc acaatatatt
     7261 tgaagaaccg tttggcgtgg tctatttcct ccgcgctttg ggcctgcatc catttggcaa
     7321 agccggtgaa cggggtgctt tcgaaataag cggccatgcc caggtaggcg taagcggaac
     7381 tgagctcgag attgagctgg ctattgagcg ccttctctat cttgcgggat aatttcataa
     7441 tgtttccatc ctaagccgga gccattcagg tttcaacttc aattcgacct ccgggcaggg
     7501 cctttggttt tgcagggcat aagccgggga ggttgaggct gcgtgttgga cggcgggcaa
     7561 gctcccgatg attggaggaa ggggcggatc ggggcaacgg tgtgccaacg gggccggcag
     7621 ggcaatggcg acggttcgcc ctcatctgcc gcaacttcgg cgccgataac aaaaggctct
     7681 gcgcgccgcg cgcggcagaa caaaaggatt gctcccgggg cgggatttat ggctttatcg
     7741 tctggacccg cgccggtggt gaggaaattt tgctcccggc gcggacggat tatggcatat
     7801 tggcgcgagt gaaccgctta gaccgcaaaa gaatgcaggt tcggtattcg ggccaagtgc
     7861 aaggcgtcgg gttccgctac accgcgcgct cggtggcggc gggctatgac gtcaccgggt
     7921 ttgttcggaa tctgccggat ggcggagtgg aactggtcgc cgaagggtcg aaagacgaac
     7981 tggaagcctt tcgggaggcc atccgcgagg cgggcctggg ccattttatc cggcacgaag
     8041 atattgcctg gcaggaagcc gggaatgcgt tccgtgggtt tgaaatcgta agatagaagt
     8101 taaatgaagt ttgcaatcat cgccgacatc cacgccaatc tcgaggcgct gcaagtggtt
     8161 ttggaggaca tcaaagaaca aaaatgcacg cactatgcct gcctgggcga cgtggtcggc
     8221 tacaacgcca atcccaagga atgcctggat atcatccgcg cgatgaaaat gccggtcgtg
     8281 aagggcaatc acgatgaata ctgctccacc gacgggccgc tggacggctt taacccggcg
     8341 gccgccgagg cggtgaactg gacgcgcaag cagttgacgg cggatgaccg ccagtggctg
     8401 cgggacctga aatacgtccg catggtcacc aacttcacca tcgtgcacgc caccctcgac
     8461 gggccgcagc gctggggcta tgtattcgac aagctggcgg ccgcggccag tttcacctac
     8521 caaaacacgg ccatctgctt cttcggccac acccatgtgc cggtggcctt catgcgcgac
     8581 agcctggtgc gcggcgggac ctactccaag ttcaaagtcg agcccggcaa aaagtatttc
     8641 gtgaacgtca gcgccgtcgg ccagccccgc gacaacaatc ccaagtgtgg ctacgtggtt
     8701 tatgatgcgg acgaagggac catcgagcag cggcggctgg aatacgacat cgccaccgcc
     8761 caaagaaaga tcctggatgc cgggctgccg ccacgcctgg ccgaacggct cgccctgggc
     8821 cggtaaggcg ccgtccgccg ccgccccggc gcccggccgc ggcccggcgg aagcggtcgg
     8881 ggggtgcggc ccgcgttgcg cctggtgaaa atcctgatcc tcaagcccag ctcgctcggc
     8941 gacgtggtgc aggcgctgcc ggtgctgcgg ctgctcaagc ggcatcggcc cgacagtgaa
     9001 atctattggt ggattgatgc ccgcctcgcc ccgctgctgg ccgatgaccc cgacctggcg
     9061 gggatcgtgc gcttcgagcg ccagcgctgg gcccggccgt ggcactgggg cgaaatgggg
     9121 cgcagcgtgc gctggctgcg gcggcaggcg ttcgactggg tggtggattt gcagggcctg
     9181 ctgcgcagcg gcgccttcgc ctggctggcc aacggcgggc tgaccgtcgg cctcgacgag
     9241 ccccgcgagg gcgggcgcgg gttttatgat ctcatcgcgc ggcgcccgtc gcccgccacc
     9301 catgccgtgg actggtaccg ccaggtgctg ccgctcctgg gcgtgccggc ggaggcggag
     9361 tttcagtggc tgcccgagcg tcccggcgtc gcggccgcgg tgcgccgcaa atggccggtg
     9421 gaaccggcgc gctggattct cctgcaaccc ggcgcgcgct ggctgaataa acgctggccc
     9481 gccgagtcct ttgccgggct ggtccggtgg ctggcggcgg cggaccccag cctgcggttt
     9541 gccatcctcg gcagcgcgga cgaccgcgcc ctgggccagg ccatcgcccg ggccggagag
     9601 cggcggtgcc tcgatctgac ggggcaggtc tccctcccgg aactggtcga atggatccgg
     9661 ctcagcgcgt tactggtgac caatgacacc ggcccgatgc acatcgccgc cgcgctgggc
     9721 aagccggtcg tggcgctgtt cggccccacc gacccctgcc gcaccgggcc gtatcggcag
     9781 ctcgaacacg tcctgcaact ggacctcccc tgcgtcccct gcctgaaacc ccgctgcacc
     9841 tacgtccggc cgctggaatg tctccgcgca cttccgcccg ccgtcgtcgg cgcggccgtg
     9901 caaaagcgcc tggccggcgc ctgaagcccg cgcgcccgcc caccccgccc ccgccggcgc
     9961 gggtactttt gcggcttgcc acggcgccgg cccgccttag agtggcgcca tgaaagcttt
    10021 gagactggcg ctttgcctgc tggccgccgc cgtggggccg gtggcggcgc aggtcaccgt
    10081 ggaagtaatt caggagcaag accagttcct gccgggagaa gccatgccga cggcggtgcg
    10141 cattacgaac cgttccgccc gggatctgcg cctcggggcg gagaaagact ggctggtctt
    10201 ttccgtgcaa acccgcggcg tcgaggtggt gcccaagctg ggcgaggtcc cggtcgccgg
    10261 cgagttcatc ctgggctcgt ccaaagtggc cacgaagtac gtggatttgg cgccgtactt
    10321 tttcatcaac cggacggggc gctacatcgt aaccgccacc gtgcggatcc gggagtggga
    10381 ccgcgaggtc accagcccgc ccaaagagtt caatgtctcc gatggggcca agttgtggga
    10441 gcaggaagtc ggcgtgccgg ccgccccgga ttccgccccg gccgtgccag aagtgcgcaa
    10501 attcatcctc cagcaggcca gctccctcaa gacgcagcct cgcctgtatg tgcggctgac
    10561 cgacgccacc ggggcgcgga ccttccgcgt cattcccgtc gggcccaccg tctctttcgg
    10621 ccggccggaa ccgcaagtgg acaagttcag caatttgcat ctcctccacc aggagggcgc
    10681 ccgctccttc agctacgtcg tcatcaatcc ccagggcgaa atcatcgcgc gccgcacgta
    10741 tgactatggc gataaacgcc cccggctcca gccggacatg gacggaaaga tcatcgttca
    10801 gggcggagtc cgccgcccca cccccaaaga cgtgcccccg ccccagccgg cgcccgcggc
    10861 ggaagaagaa cccaaaccgc tgcgctcgcc gggagagttg gtgcccccca acctttgacg
    10921 gaacatcccc cgccggcgcg gcccgcgccg acggcgtcgc aaatccccgg gtcaggattg
    10981 gacggccagc gccgacggtt gcgcccgcaa ggcgaggatt ttggccacga ttgcttccag
    11041 gctgggcagg gtaaagtcac ggcgcgcatc gcccagccac ggcgattgca gcagcagcga
    11101 cgtgcagccc gccgcccggg ccatttcggc gtccggccat ttatcgctga cgacaaaaga
    11161 ctggtccagg tccacttgcc atttgaacgc ggcctccaac aacagcccgg gcttcggctt
    11221 gcggcagggg cattggtcag ccgcctcatg cgggcatacc aggatatcat ccagcgggaa
    11281 aacgcgccgc agcaggtcgt gcatccgatc cagctcgcgc cgcgattgat ccccgcgcga
    11341 caggcccggc tggttggtcg tggcaatcag caggagaccc gcggccttta attggcgcag
    11401 caagggtgcc gcctcctggt tgacctgaaa atcctccagc gtcagcgggc tgacctggtg
    11461 ccgccgctcg atgcgcaccc ggttcagtat gccatcccgt tcgataaaca cacccggttt
    11521 cacacttcgg agacgcataa agctttccca atcagcgttt ttcgcccaaa aaccccgatt
    11581 ttccggccct aaccggtggt ttttcgcttc cgccagtcgc aaaactgaga aaatcccggg
    11641 cctgatctcc gccccggcgc gcccggccgc tgaggcgcgg ccctcctccg caaggctgcc
    11701 gcgcccgctt attgcaggtc gttaagcgcg gtcacccgct tttgcagttc ccggatcacc
    11761 ggcaaggccc gggcatagat atgctgttcg gtttcgcccg gagacggctt caggatcacg
    11821 cgcacggcgg cgatgggttc gccattccga tcgcgcagcg gcaacaccgc tgaaaccgac
    11881 gtccgctctt tcccacaata cggcgagccc tggtggacga cgtcccactc cacctttccc
    11941 cccgcgcggc ccacctcggc cgggttctgg ttggccacaa ggcgcgtcgt ccgcggttcc
    12001 tcgccccgca catagacttg caagccgagc aaccggggaa acttcttcac cgccgcgcgc
    12061 accagcgcct gggccggggg ctcgtgcggc gtgtacacca gccgcgtatc ggcaaaataa
    12121 ctgaccgcgt cggacttggt ccaaaacccg atcttcccgc tggcgaacgg attaacccga
    12181 tccgtcgcgg tgatcaattc ctggccgttg aggcggcacc ggatggaatt gcctttgcac
    12241 tcgatcgtca gctcatgcca gacctcgctg ggcaccggca gttccgggcc gaccagcggg
    12301 ccgcgttcgc cgttgagcac cttgtagaag cgcaggttgt tgccccggga actcgcccgc
    12361 acgacatagt aattcgtctc gttttgaatc cggaaagcaa tgcccgccat ctgttccacc
    12421 gcgcccttaa cggtcttaaa gcgggtggtc agctcgaagt cccccatcgt ttcctcctcg
    12481 taaatcagca gcgggaaatg ttcatccgtc gggtcctgcg ccagttgcgc cagaaccgcc
    12541 ttccgggcgg ccgaccgcgc ccccggcgtc agcgccggca gcaggggcgg cacctcgtcc
    12601 caaatcaccc gccaatcccc cggcttgcct tgcccggtca cggtgctgcg aaagccgggc
    12661 ggcacctggc cttcgcgcac ttcgctgaag tcaaacttcc gttccgccgc cgcaaccggc
    12721 agcaccagca aagccagcgc ccagtggaaa aaggttcgca tgcggcccga gcgtagcaaa
    12781 acggcgcccg gcgacaagcg caaggtaggc cgcccgaaaa cggtttgccg ctcctcccgg
    12841 cccggcccgc cgggcgggcg cctccgccgg cgtggttttc gcttggcgca accgccacgc
    12901 cggccctagc cgggatattt cacgccccgc acgaaatacc cgcaggccgg agcgcgggca
    12961 tcttgcccgc ttagcccgtt tggaggctgt aagcgggcaa gatgcccgcg ctccagcctt
    13021 gcaggtgtga aatgtctggg ctagccttgc ccccgaatta gcaccggcaa tccctcctcc
    13081 cggaggtccg ggcgcgcgga ggcgcccgcc ctcccggcgc gggtttgccg agtgtttgct
    13141 tatggccgcc agcgacccaa tgcgattatt cattgcgata gcaatacccg aaccggtcaa
    13201 agcccggctg gaaaccgcgc aaaccgaatt gcgccgcgcg ctgccgggcg ccagcgtccg
    13261 ctgggcccgc cgcgagcagt tgcacctgac gctgaagttt ctgggggagg tggcagccgc
    13321 gcgcgtggaa ccgctggccg aggcgctgcg caacgcgtgc cagccattcg cccccctgcg
    13381 gctgcgcctc gagggcgtcg gagggttccc caacctgcgc cggccgcgcg tcctctgggc
    13441 cggcgtgccg gatgaagcgg ggcggttggc gcaactccag gcggcggtgg acggcgccac
    13501 ccgggatttc tcgaacgaaa agaaagagcc tggattcgcc ggtcacgtca cgctggcccg
    13561 cctcagagac ctgaaccgaa ccgaagcgga cgccctagcg gaacgggcgg acggcatgaa
    13621 acagcagcca tttggccagt ggaccgtgga ccagatcaag ctaatgcgaa gcgagctctc
    13681 gccccaggga gcacgccaca cccccctggc catcttcacg ctggcggcag ttgatacccg
    13741 ttaacgggct ggccgggcac agccaaaatc ggtcccgtcc gcccagccaa aatcgggcta
    13801 atgcaacgac cttaaccctt agagccccct ccgaactgtc caattattgg acacttcccc
    13861 cttttcgact ctcgtcattc tcacgcactc cgtttttcgc gtaactcccg tctttccagc
    13921 ctcatccata cacgccatcg agccgtgtac aaggggtgta caagggatgc tcatcgggtg
    13981 taccagctaa aatcctatga acttccggtg agcatccctt gtacacccct tgtacacggc
    14041 tcgatggaag ggggacggga ggggatgggg aggttgctgt tggcgggaga ggcaggaggg
    14101 gaaatctggc ctggttgcct gggaggggtt ggatcgcagt gcgtcggggg agcgcccggg
    14161 tgatgctggt caattccggg ctgggagagc ggggtcattc gcgggctctg gtttcgagaa
    14221 ccttcggggt gcgcgtggat ttggtttgac ctgggggccg ccgcccggca gactgcgctc
    14281 cagcaaacga tcttaacatt acgaacatac agcatctctc atgagccaaa catccaatca
    14341 gcgatccgac gacttcctca agattagcga ccaatttcaa ctgggggtcc ttacgactga
    14401 atcctcccat cccgtcaccg ccaacctgag cgaaacggcc aaaggcgacg tggctgcggg
    14461 tttggggctg ttgttcgagg ccgatgggga taatgtgcgc aagtaccggg aatttgcgga
    14521 gtcggggcgg gcgcggcaga ttgcggatac cgtgctgcgc gtggtgaagg ggggcgggaa
    14581 ggtgtttttc accgggtgcg gctccaccgg gcggctgagc attcaactgg tttccatctg
    14641 gcgcgatttc tggcagaagc agcgcgcgcg cggcctgcag agcaagccgt cgccggagga
    14701 gttcgagcac cgcgcgttca gcgtgatggc gggcggggac ttcgcgctca tcaaagcggt
    14761 ggagggattc gaggatttca cggagttcgg caagaagcag attggcgacc tggggatttc
    14821 ggcccgtgac gcggtttttg ccatcaccga aggcggcgag acgtcgtttg tgatcggcac
    14881 cgcctggaag ggggtggagg tgggggccaa ggtctatttt gtctataaca acccggatga
    14941 gatcctgtgc aagcatgtga agcggagccg cgaggtcatc gaggagccgc gcattgagaa
    15001 gatcaatctg acgaccgggc cgatgtccat taccggctcg acgcgcatgc aggccacgac
    15061 cattcagctt tgcgtgatgc tgacggtgct ggagatggtg ttgcgcgacc tgatgaagga
    15121 actggagccc caggggccct gcgcgctgga ctccaacgcc gtgccggcgg agtttctccg
    15181 gaagctgacg gagatgcacg gcaatctgcg ggcgccggat ttccgcgcgc aactggcccg
    15241 gatcgtgtcg ctggaagagg gcgtttatcg cgccaagcac aagaacaact actatgccga
    15301 ccggttcggg attgacgtgc tgacggatac caccgagcgc tcgcccacgt tttgctcgcc
    15361 gccgttccgc aaattcgatg atacgacggc gacggagtcc tggtcgttcc tgttcctgcc
    15421 gtatgcggac accccgaagg cctgggagcg gatcatcaag cgccagcccc agtgcgtcga
    15481 gtggaccgag gcggaagtgc gcgccctggt cagcgaggac aaggtggcgc gcacctacga
    15541 aatcgtgcgc aagatcagcg cggcggaatt gatgcggttc aaggtggggc tggacggcct
    15601 ggcgcaccgg ccgctgcgcc cgggcgacag cgcggtggcc attttgtcgg agaccgaaaa
    15661 ggcggccctg ctggcggagg acggtttcta ccgccggcaa ctggaagcgg cgaaaaaggc
    15721 gggggcgcgg accgggttga tctacgtcgg ccgtgcggag accttccccg agattcgcga
    15781 ttttgccgcc cggtggaacc ccgagggtgt gctggtgctg gcggcggtgc cggccacgga
    15841 tttcctgctg gacggcgtga cgcgcgcggg gctcaagatg ctgttgaacg cgctttcgac
    15901 ctgcacgatg gtgcggctgg ggcgcgtcat gggcaactac atgatttggg tggtgccgag
    15961 caatctcaag ctcattgacc gctcgacgcg ctacattcaa cggctcacga atctggacta
    16021 ccgggcggcc aaccgcctgc tcttcgaggt cattgagtat gtcgagccgc gcatgaaggc
    16081 cgaccaggcc tacccgccgg tggtcggggt ttcggtgatg cgcgtccgcc accagctcga
    16141 caacgcggcg gcggagcagc ggatcatggc cgagatgtaa gttgccgcta tacagccggg
    16201 ccggattcga ttacgctgga ggcatctatg gcactggatg actttttatt ggaagcggac
    16261 gataaaatgg gcaagaccga gcaggtgatc gtaaaggagt ttgccggggt ccgcaccggc
    16321 aaggcttcgc ccgcgctggt cgagaatatc atggtggagg tctatggctc gcagatgcgc
    16381 attcgcgagc tggccggcat caccaccccg gagccgcgca cgctcagcat ccagccgtgg
    16441 gatgccggct cgctgcaccc catcgaaaag gcgattctca aaagcaacct cggcttgacc
    16501 ccgtccattc agggcaaggt gatccggctg tttttccccg aactgagcga ggagcgacgc
    16561 caggaattcg tgaaaatcgt ccgcaaaatg gccgaggaag gccgcgtggc catccgccac
    16621 gtgcggcgcg atacgatgga tcagttcaag aaacacggcc acgacagcgg catcaccgag
    16681 gacgatgtca agcacgccga gaaggaattg cagaagctga cggacgatta catcgccaaa
    16741 attgacgccc acgtggctag caaggaaaag gaaatcatga cggtctgacg cggcggcggg
    16801 cgccgccggg cggcgcggcc gcttgcgcgc tcccgccggg cgtcccgggg ccgcgtcccc
    16861 gggctatttc ccgatacaga actggctgaa gatcgtgtcc agcaggtctt cggtggtggt
    16921 ctggccgacg atttccccga gggcattgac ggcgatgcgg aggtccagcg cggcgagctc
    16981 caggggcgcg cccgcgcgca gggtttcgcc cgcgcgctgc gcggcggcgc gggagcggtt
    17041 gagggcatcc tcgtggcggg cgttgatcat caccggcagc atcccggcct ggatttcccc
    17101 ggaccagacc agccccttga tggcgtcctt cagcgcctcg atgccctgtc cggtcaggca
    17161 gcagacatcc accaccggcg cgcccgccgc ggtcggcaat tgcagccgga cgggcaagtc
    17221 catcttgttg cggaccagga tgcgccgttt gccggcctcc gcggcgaggc gctcccggtc
    17281 ggccgccgtc agcggctcgg acgcgtcgaa cacgtgcagg aaacattccg cctcctccag
    17341 cgcccggcgg ctgcgccgga tgccctcggc ctccacctcg tcgcgcgcct cgcgcaggcc
    17401 cgcggtgtca atgaaaacca ccgggaggcc gcggaggttg gcggtttcct cgatagtgtc
    17461 gcgggtggtg ccggggaggg cggagacgat ggcgcgatcg tggccgagga gctgattgag
    17521 caggctcgat ttgccgacgt tgggccggcc gatgatcgcc acgcggatgc cccggcggag
    17581 caactggccc tccggcgcgg tgcgcagcaa ttcgtccatc tgcgcttcgc agtgttggag
    17641 gcgggcgatc aactgctccc gcgcatccgg cgcgatgtcc tcctcgggga agtcaatatg
    17701 cgcttcgagg tgggccagga tttcgcgcag gggatcgcgc agcgcggcga cgcggcggga
    17761 gagccggccg gcgagctgct cgttggcggc gcgcagggcc agttccgtgc gcgagtgaat
    17821 caggtccacc acggcttcgg cctgggcgag gtcaatccgc ccgttgagaa aggcgcgcag
    17881 ggtgaattcg ccgggcgccg ccaggcgcgc cccgctggcg agcaccgtgg ccaggaccat
    17941 ttgggcgggc aggaggccgc cgtggcaggt gatttccacc acctcctcgc gcgtgaaagt
    18001 gcgcggcgcg cgcatcaccg ccagcagcgc ctcatccacc gtctgcccgt cgcggacgat
    18061 gtggccgaag tgcagcgtat gggaggccgc cgccgaaggc cggggcgccg cttcgccggc
    18121 cggccggaag cagcggtcgg ctatcgccag cgcctgcggg ccggaaaggc ggatcaccgc
    18181 cagcccgccc acgcccagcg gagttgcaat cgcagctatg gtatcgtcgt gcatctcacc
    18241 acccgcgcgg cagttccggc tttgaaaatc tgcggcgctg atgaatagtg cggccatgat
    18301 gcgccattcg cactcccggg acgagagata tttgagccag gccggcggct ccggccgcgc
    18361 cgcgcggaaa accgcgcccc cggccgcggc gcgcgcggac gccgcgccgc gccggctcca
    18421 ggtgttcgtg ttcagcgagc cggagcgcgc tggcggctat ggccggaact gggacggccg
    18481 cgctgtcggc attggcgggc ggggcgcggt gctgggcgat cgtggcagcg gctacgacct
    18541 tggggtgcgc gcgcttcagg cggtggcggc ggcctatgac cagcgcgggg tctggccgtc
    18601 cctggggcgg cgtctgctcc gggcgctgca gcttaatgaa cccggggatt taatggactg
    18661 ggcccggacg gccgaaaggg aggaaatcgc ccgcctcgcc gccgaagtct ttgccgcccg
    18721 gagccggcgg gacaagctgg cggcggacat cctggcggac gccgccgggc aactggcgcg
    18781 gaatgcggcg gcttgcgcgc ggcggctggc ccggcccggc gttcgcatcc agtttgtgcc
    18841 ggccggcagt gttttccggg cgcatccgtc ctttgcggcg caagtggggc gcgccgtgcg
    18901 gaaactttgg ccccgggccc tcgtggcgtc acaggtgctt gaaagcgttc cggacgcggc
    18961 ggccccggtt aagcgggagc ggtcctccgg ggcgcgcggg gcgcaaaacg ccgtccgcgg
    19021 aaagggaaat aaaaaccccc gtccggaggc cgggccggag cgggtgcggt cccgccgccc
    19081 ttcgcccacc gaacagcgca atccgcgctc ccggcgcctg gacaccctgt ccctgcagcg
    19141 ggcgatccgg ctgatgctgg ccgaggaagg ccgcgtgccc ggcaagctgc aggcggagca
    19201 tcgccgtatg gcgcggctca ttcaggccgt cgttcgcgcc tttcggtgcg gcgggcgcct
    19261 gttctatgtg ggcgcgggca ccagcggccg gctgggggtg ctggatgcca gcgaatgtcc
    19321 gcccactttt ggcgccccgc cggggcaggt gcagggcatc attgccggcg gccgtccggc
    19381 gttgtggcaa tcggtcgaag gcgccgagga ccaggcggag gcgggcgcgc gcgccctcgc
    19441 gtaccggggc ctgggccggc gggatgtggt cgtgggtctc gcggccagcg ggaccacccc
    19501 ctttgtgtgg ggcgcgctgg acgcggcccg gcggcgcggc gcaaccaccg cgttgatctg
    19561 cttcaatccg ttcctgcgga ttccgcgcgc gcggcgtccg gatgtggtca tcgctccgaa
    19621 tctcggcccc gaggtgctga cggggtcaac ccggctgaaa gccgggacgg cgacaaagca
    19681 actcctgaac atcctcacca ccctggccat ggtgcgcctc ggcaaggtgc gcagcaacct
    19741 gatgattgat ttgcatccgg ccaacgccaa gctgcggggc cgcgccgtgc gcattgtgcg
    19801 ggagttgacg ggggccgatg ccgccggcgc cgaagcggcg ctccggcgcg ccgggtggaa
    19861 catcagaaag gcaattgcgc tgccaggtgc gaaaccgcgg actggagttt ggggaacttg
    19921 cccgcccagt acgcgcccgt gataaaccgc accacgccgg tgaggagcga cagcagaagc
    19981 ccgctcagaa agtacagact ccaggcatag gacgggtcgg gcttcatgac ccggggaacg
    20041 ccatggtaga gcactcccag cgacagcacg atgccgatgg cccagcttgt ccagggggaa
    20101 gggacgggga ataaatccac caggcgcagc aaaaatagcg gtcccaggcc gtaggccacc
    20161 gttcggaacg cctgcgtgta ggagggttcg ccccggaacg tttcgccgac ctccttgagc
    20221 aggcgggcgc ccgcaaaaac caccagcagc gcgagcagga cctgcgcgct ttccaccacc
    20281 acggcctcgc ccacactgat ctttttcagg cgggcaacct cgccctgcca ccggccccaa
    20341 ttagccaggc catacccctc gattccggcc gtgagcagga gcaggggcaa caggaagaag
    20401 ccaagaatga agagtacgcc gcgctgggcc tgttcaattt tgtcccaggt cacttgcggc
    20461 tcaaagatca gcagcaatac tttaatcatt ccagggccac tttatcggga gatcccaccg
    20521 cctgacaagc cgctaccggg agcgccggcg gaagcccggg cgggacggct cccgcggaga
    20581 tcggtggcgc gcgggcgggt gaattccctt ttacacccgt gggatttgtg gttaattcaa
    20641 cgcggtgaaa gtcaaccttg gcatttggga caaattgacg ccggtggtga ttttcctgct
    20701 gttcatcgcc atcatcatgc tggtgggcat gtggtatctg ccgcgcatcc agcagaacca
    20761 gcggatgcgc aaggaaatcc tgcggctgga gactttgatc cgcaaggaag aggagacgct
    20821 tcggcagttg cgctcctccg ccgatgcgtt gcttcacgac accaagaccg tggagcggct
    20881 ggcccgggaa cggctgggct atgcccggcc gggcgaaatc atcattcgtt ttgaagagcc
    20941 ccgctccaac ggcgtgagcc ggcgctaagc ggcgggaacg ggcggcgggc ggtgttccgg
    21001 cgggtgcggc gcggcagggg tttgcgccgg ccgggagggc ggcggggagc gttccggggt
    21061 cgccccgttt tcctgctccc gccccaggtt gaacttgatc caggtttggg agccggtctt
    21121 gcggttgcgg atttccatct ccgccagttg gtattcgccg tccacctttt ccaggctccg
    21181 ggggtcgaag cgtttgagca ggctgccctg ggcgtcgtag gcgtcggcgt gaatgagccc
    21241 gtgcgggctt tcgaccatga accaggccac cacgcgggaa taaccgccgg gcacgggctg
    21301 gggattgatg ctttcgagca ccttgcagga tttgccgtgt cgcatctcgt atttgaggac
    21361 ttgctggcgc ggccaatgca gaaattccag gccgaggtcg gcaatccaga agtccgagcc
    21421 ggcaaagggc tgcatgattt cggccgccgt caattcccgc gcggcgggag gcggagcgcc
    21481 gccgtccgcg gggtcgcgca attcataccg gttgggctgc tgcgccgcgc gggtgatcgt
    21541 cagccggacg ccgccggggg agtccggcgc ggggagcgtt tcgtaagcgc tgacccaatg
    21601 ggtgggggtg aggaagacct cgaagcgggc ggggatttgc cgctctttgc ccgcggcgtc
    21661 gcggatgcgg acccagccgg tgttggtgga attgcgttcc ggtttcagcg ccagcaactc
    21721 ggccaccagc gcgcgcgctt cctgcgctcc gcgctccggg tccagcggcg cgggcgcccc
    21781 ggccgggtgc cgctggccca ggctggcggc cgcgccgccc aggagcagca ggaggatgcg
    21841 ggcccagacg ccgaagcggg cggggttcgg tttcatgacg cgcgcagcgc cagcggcagc
    21901 agctcggaaa tgtgccggat gaaatgcgct tgcagcttgc ggcggacttc ctccggcact
    21961 tcgctccagt ctccccggtt ctgctccggc aggatcacct gcttgaggcc gaagcgcgcc
    22021 gcggcgagca ctttttcttt aatgccgccc acgcgcagca cccggccgcg caggctgatc
    22081 tcgccggtca tcgccacgtc cgagcgcacc cgccgccgcg tcagcaacga tgccagcgcc
    22141 gccaccagcg gcacgccggc gctggggccg tccttgggcg tggcgccggc cggcacgtga
    22201 atatgcaggt cgtattgggc gtagtcggcc aggtcgagct tcagcgcccc ggcctggctg
    22261 cgcaggtagc tgatcgccgt ttgggcgctc tccttcatga cttcgcccag cgagccggtc
    22321 agcagcaggt ttcccttgcc gggcatccgc gtcgcctcga tgaacaggat ttcgccgccg
    22381 accggcgtcc aggccaggcc ggtggcgatg cccgcttcct tgatggtttc cgcggtttcg
    22441 ttggggaacc gcgccgttcc caggtagcgt ttcaacgtat cggggcccat caccagcggt
    22501 ttggccgacg ccccgtcgct cacgaggcgg cgcgcggcct tgcgcgccag ggcggcaatt
    22561 tcccgttcca attgccgcac gccggcctcg cgcgtgtagt cgtggatgac ccgccgcaag
    22621 gtggcgtcgg ggatccgcac ttccttccgg gccaggccgt gctcttccag ttggcgcggc
    22681 accaggtagc ggcgggcgat ttgcagcttt tcggattcgg tgtagctggg cagttcgatg
    22741 acttcgagcc ggtcgcgcag cgccgggtgc accgggtcca gccagttggc ggtcgtcaaa
    22801 aacaggacgc gcgacaggtc gaacggcaaa tccaggtagt gatcggtgaa ggtgtggttc
    22861 tgcgcggggt ccagcacctc gagcagcgcc gccgccgggt cgccccggaa gtccgcgccc
    22921 accttgtcca gctcatccag caggatcacc gggttgcggc tttcgacgcg tttcagggtc
    22981 tggatgatgc gtccgggcag cgaccccaca taggtgcgcc ggtgcccccg aatctccgct
    23041 tcatcccgca tcccccccag cgcaatgcgc gcaaacttcc gtcccagggc gtcggcgacg
    23101 ctccggccca gcgaggtttt gcccacgccg ggcgggccga ccaggcacag gatcgggcct
    23161 ttgatctgct tgcgccgttt gatgaccgcc aggaactcca gcagccggtc tttgaccttg
    23221 cccagcccgt agtgctgttc gttgaggatt cgctcggctt ccgccaggtc aattttgtcc
    23281 tcggtttccc gttcccacgg caggcttaaa atccagtcca ggtagtggcg gctgacgccg
    23341 tattccgccg ctgccggcgg catctgctgc aggcgctcca gttcctgggc cgccgccttc
    23401 ctcgcctcct ccggcatcgg ggtttgctcg atgcgctccc gcaggaggcg gatctcgccg
    23461 gcgttggggt cggactcgcc cagctcgcgc tggatcgcgc gcaactgttc ccggaggaag
    23521 aagtcgcgct gcgtcttgga gatggacgtc gccacgtcgc tctggatttt ggagctgagc
    23581 gtgagcactt catgctcgcg gttgagcatg ggcagcagcc gcgtcagccg ctccttgacc
    23641 gagttggttt cgagcaattt ttgccgttcc tccaggttga ggttgaggtt gacggcaatc
    23701 aggtccgcga agtgtcccgg gtcctctgtg ttgagcgccg cgattttgat ctgctcggac
    23761 aatgccggcg aaagcttgat gatctcctga aactggcttt gcgcgttgcg catcatggcg
    23821 gtcagctcca cggagtcttc cttttggtcg cgcagcagtt cgagccgggc gcgcaggtac
    23881 gggtcggtcg cgggatattc cttgagccgg atccgccaca gcccctccac cagcacgcgc
    23941 accgtgttat ccgggaactt gagcattttg agcacgcgcg ccgcgcagcc gacctcgtgc
    24001 atttcctccg gccggggatt ttccgcctcc gcgttgcgct gcagcgccag gcccagcagg
    24061 cggtttccgc cgaccacgtc gtcaatgagt ttgatgcttt gcgcggtctc caccagcagc
    24121 ggggccacca tgccggggaa aaccacaatg tcggacaggc ccaggatcgg caggatctcg
    24181 ggcagcaccc gggaagagac gcgctcgggc gtctctgcgt tgccggccgt tccgagaata
    24241 ctgataaatt ccgtatcggg cgaggtcatg attaagccaa cccgcggatt agacgccggc
    24301 gcccggggag aacttcagat cggaagtcaa tccccctcgg ccaccagcac ccgggagcgc
    24361 ttcactggcc gttgcgccaa gggcacgtct atccgcaaaa agccgttcag gtacgctgcc
    24421 ttggcctggc tcaaatcata gtccgacggc acctccagca ggctttcaaa cgggccatag
    24481 ctgatctcca tgacgaggaa actgcaatgg gccgcgcggc atccgtccgg ccggttgccc
    24541 gcgattcgca gccggttccc ttccaccgtg atttcgaggt gctcgctgcg catccccgcc
    24601 agctccacct ttaccaccag cccgctgtcg gttgtgtaaa cgtccgtgtt gggcacccag
    24661 tgggcccggc cgggttcggc ctggctgccg gcggtcgagc gggtgacaaa atgcaccgac
    24721 gaactgactt tgcacatggc catgttcagc gcgcgaagtt atagcctgac cccggcggtt
    24781 ttacaagggc gtttttcacc gcagcttcaa ccgcacttcc gggcgctccc ccttgcgcgc
    24841 gcgaaacacg atcgcgcccg acggcccgga actcagacgc tccaggcaac cgcagcgcga
    24901 gacgcgctcg aaacaatact tccgccggcg aaatcggctt ccgagcagca gcgtggctgc
    24961 ggatgcaacg attgccaacg acaccaactg ctgccaatcc atgccgacaa cataacccga
    25021 aatcgccgcg gcgcaactgg gcgcttttcc gtgttgggcc gcgccttaaa gataacgaaa
    25081 gtgcactgcg gctcccgccc gcgcggatct gggagccgtc tccaccctcg cgccgaagat
    25141 ggttctccgg cccttgccag cctgacccta tcgggggcgt tgacatctgt tttcgtccgg
    25201 agttgcgtta actcactgtt tccacggcac ttttgctact ggctctccac tggcgccggc
    25261 cagtcatcca tcaacctgct ctgagggaaa ccgcaactcc aatcacccca gggactaaaa
    25321 gattattatt tcggagtaaa gcggcgtcgt tgcgggaggc aggatttttt gcggcgtcag
    25381 gccggcggcg attcccgtta aaaagagggg cggccactgg aaaggggtgg tcgcgggtga
    25441 ttggattccg cgccttgtgc cgcttccgcc tcttggttaa ggtcgttgcg gcatgagccg
    25501 caaaattgcc agtcaatggg tctgttacct ggcggtcgcc gctttggcat taggaatgca
    25561 agggcatcgc gcctccgcca gccagcttct tcctccgggg tttcgtccgt tgccgctggg
    25621 cacccatgcc ctgaccggcg ggcgaattgt catccgcccc ggcgaggttc tgacaaacgg
    25681 caccttggtc attcgcgagg gccggattgc ggcggtggga aaagacgtcg cgccgcccgc
    25741 ggacgcccgg gtctgggaga tgaaaggcac gacgatctac gccggattca tagatctcta
    25801 tctcccgctc gaagcaacca actcggcggc ggcttccgcg ccgggcgaag cggctgccgg
    25861 ggacgggctt accgcgagcg ggccccgatt cttcggcgtc ccgggcgcgc aaccggatcg
    25921 cggtcctgcc gggcccggct atgctgtggc gggcattacc ccggagcggc aggcggtgcg
    25981 gagctatgcg cccaaggccg ccgccctggc gcccatgcac gcgctggggt tcaccgccgg
    26041 cttggttgcc ccggcccggg gcatcattcg cggcaccagt gcgctggtcg ccttgtccgg
    26101 ggatgctccc aatgcggtgg tgattaagcc tgaggtcttc cagcacatcg gcttcacgcc
    26161 gatggagagc gcggaacgcg tttatccttc ctcgctgatg ggcgtgatcg ccgcggtgcg
    26221 ccaggcgttc ttcgacgcgc gctattacgc cctcgaccag gcggattatc agcagaatcc
    26281 gcaagcacgc ccgcgtctcg agttcaatcc cgccctcgcg gcgctggctc ccgcggctga
    26341 ccggaagcag ctcgttgccg ttgagcccgg cagcgtcttg atgaccgacc gcgccgcccg
    26401 catcgcgcac gaactggggc tggatttctg cctcattgcc agcggccagg agtggcgccg
    26461 ccccgatctc gcccaggcca cgggcgcggc gttcattgtg cccctggatt ttccggaact
    26521 gccgagcctg ccggacgaag acgactggga gcaggttcaa ctggattcgt tgcgggcttg
    26581 ggattgggcg cccgaaaatg ccgccgtgct gcggcagcgc ggcttggaaa tcgccctgac
    26641 cacctggagg ctggcggaca aaaagaagtt tcgccccaac ctgcgccgcg cgctcgaccg
    26701 cggcctggcc gaggttgacg ccttggccgc gctgaccacg gttccggcca aactctgcgg
    26761 cgtcgcaagt cagctcggca ccctcgaacc cggcaagctg gcttacctca ccgtcgtgga
    26821 aggcggcagc tactttgacc ccgaggccaa agtccgcgag gtgtggattg acggccgggt
    26881 ttacccggtg ccgggcgagg aacgcggggc tgcccaagcc ggggcgagcc agtcccggac
    26941 tgccgcggcc aataaaccga agccggcgaa ggtggccgcg gataatcccg gagaacggcg
    27001 ccgtgccttg caaaagaccc ggctggcgcg ctcgccgctg gaagggcgcg gaccgctggc
    27061 ggagccgaag gccatcctga ttgaacaggc caccgtctgg acctgcggcc ccgaaggccg
    27121 cctggaggac gcggatgtgc tgttcgtcgg cggcaaggtc aaggcggttg gcaaggggct
    27181 ttcccggctt tcgccggatg gcgatccgcc gctggtgatt gacgggcgcg gccggcacgt
    27241 cacgcccggg ctgattgatg cgcatagcca caccgccatc ctgggcgccg tcaacgaatc
    27301 gtcgctgccc tccacggcca tggtccgcat ccgcgacgtg gtgaattccg agacggccaa
    27361 cctgcgccgg cagctcgccg gcggcgtcac cctcgtgaac ctgttccacg gctcggccaa
    27421 tccgatcggg ggccagagct gcatcatcaa actgcgcgac ggcgccggcc cggaggacct
    27481 cgtcttcgac gccgcgccgc cgggcatcaa gtttgcgctg ggggagaatg tgaagcaatc
    27541 cggctggggc gaccgctttg tcacgcgctt cccccaaacc cgcatgggcg tgcgcacgct
    27601 gatagccaac cgcttcatcg ccgcccggga atacctggcc cgccgggagc aggcctccgg
    27661 ccggtccgcc ctcccgcccc ggcgcgacct cgaacttgaa gccctgggtg aagtgctgca
    27721 agggcgccgc tggattcatt gccacgcgta tcgccaggat gaaatcctca tgctcatccg
    27781 cctgatggag ggcttcggcg tgcggatcgg cagcttcgag cacgcgctgg aaggctacaa
    27841 agtggccgat gagattgccc ggcacggcgc ggccggctcc accttctcgg attggtgggc
    27901 gtacaagttc gaggtctatg acgcgatccc ctataatggc agcctcatgc gcgaccgggg
    27961 cgtgctggtc tcgttcaatt ccgattcgtc cgagctggcc cggcggctct acacggaggc
    28021 cgccaaggcg gtgaagtatg gcggcatgcc cgaggtcgag gcgctccgcc tggtcaccct
    28081 caacccggcc aaacatcttc acctggacgc gcgcgtcggc tcgctggaac cgggcaagga
    28141 cgccgacttc gccctttggt cccattctcc gctcgattcc cgcaccgtct gcctccaaac
    28201 ttggatcgag ggaaaaaaat acttcgaccg cgaacaggag gccgggcgcg ccgcccaact
    28261 gcaagcggaa cgcgccgctt tgctggcccg ggcccaaaag ctggcgaagc tcgccagcgg
    28321 cggcggcgcc acttcggagg aagcgaagaa ctccttcttc cgcgtggccc tggaacacga
    28381 gttcgacggc tgcgaccgcc gcgaactgga ggagtaagac gcatgcgtaa gttcatttcc
    28441 cattttcggt tcgagagggc gggcctcccc gcaaccttca accgccgccg gttcgcttcg
    28501 ccgccagggc gtgcgcgggc gctcaccttc ccgcggcggc gcggatgcct ttcgctgtgc
    28561 ggggcggttt ggcttgccgc gctgacgccg ggcgtggcgg ctgaaagcct cctcctttcc
    28621 ggcgccatcg tgcataccgt tgcgggtgaa accctggccc ccggccaggt gctgatccgc
    28681 gggggcaaga ttgccgccgt tggccaaact gttcccgccg acggcgcggc ccaggttgac
    28741 ctcgccggcc ggtatctcta cccggggctc atcgcgctca acaccgccct ggggctcacc
    28801 gagatcagcg ccgtgcgcgc cacccaggac agcactgaag tgggggatga cttcacgcct
    28861 gatgtggaat ccgggatcgc cgtgaatccg gattcggagt tgattcccgt cgcccgcgcc
    28921 aacggaatca ccagctttga acccgtgccg caaggccacc tcatctccgg ccaatccgcg
    28981 ctgatggcgg ccgacggctg gacgaccgag caaatgacaa tccgcaatcc gctggcgctt
    29041 cacctgttct ggccgtcccg ggaacttgac ttcacgccga aagaaaaggc gcgcaaccgg
    29101 gcgcagtgga aatccctggc ggaccaggac cagaaacgcc gcgcccggct ccgggccatt
    29161 gcggacttct tcgcggaagc cggggcgtat gcccgggcca aagatgccgc cgcccggggc
    29221 caggcccccg cgccggaaat catccccgcc tgggaggcga tgctgcctta cgtgcggggc
    29281 gaacggcccg tcgtggtgca tgccgacgaa atccgccaga tcaaggccgc cctggcctgg
    29341 gcagaaaccc agcgctgccg catcatcctc gccggcgccc gcgacgcctg gatggccgcc
    29401 gatcagttgg cggcccggaa aatccccgtt ctctatggtc atgtccatac gctgccggcc
    29461 cgggacacgg atccctacga tgtgcatttt cgcgcgcccg ccatcctcca ccaggcgggc
    29521 gtccgcgtcg ccttcacgct cggcgagggc agctttgacg cggcgatgat ccgcaacctg
    29581 ccttacctcg cggcccaggc ggccgccttt ggcttgcccg aagcggaggc gcttaaaggg
    29641 ctgacgcttt accccgcgca gtgtgccgga gtggccgacc gcctcggctc tctggaagcc
    29701 ggcaaagacg cgaccctttt cgccgccgac ggagacattc tcgacatccg cagccgcgtc
    29761 cagcgaatgt ggattgcggg ccgggaagtc agcctcgaaa gccgccacac ccggctctac
    29821 gagaaataca aaaaccgtcc ccggccggag tgaaccctaa cgcgccgtca ggcgcagcat
    29881 ggacgatgca ccaggcctgg cgcggcgcca cgtcactcct agctgaaccc ggccaacgaa
    29941 tcacagtgag tggacggagt ggcagcactg cgaggtgcga gcgcgggacg gcacgggatc
    30001 tcacagttga aacggcggcg ttgcaccccg ccaacagttt ggcccggttc ggacaccgcg
    30061 tgtcaggttc cggcggccag gcttctccca cttgcagcag tgattcaaat cctgtaatat
    30121 gacccgcatg agcgaactgg tttttgaggt gacgcaagag gaagacggcg gcttctgcgc
    30181 cgaatgcctg tcggcgaaca tctttacgca gggagacacc tgggaggcgc tgcgcaaaaa
    30241 tgtccaggaa gcggtgcggg cctattattt tgaccaaccc ccggccccca aacgtattcg
    30301 gctgcacctg gtgcgggacg agctgctggc ggacgcatga aagtgccccg ggatttaagc
    30361 ggcgcgcaac taatcaaagt gctgtgccgc gattggggct atcgccagtt gcatcaagaa
    30421 ggcagccacg tcatcttgca aacggagatg ccagggcatc aaagactgtc cgttcccaat
    30481 cataatccgt tgcgcgtcgg cacgctcaac agcattgcgc gggcggtggc cgcgcacaaa
    30541 ggcgtggacc gccaggctgt gctggactcg ctgggatgac gggcgaagcc tctttttgcg
    30601 tttaccggcc ggcgtgaggg attcacggaa acgcgctcaa ggccagtgag gctcccgcag
    30661 gtgccgcttg tcattggccg gcgcagttcc catattcgat cccgcataag ctatgagcat
    30721 tgactgcgat ctcctcatcc gcaacggcac cctctatgac ggcagcggcg ggccgccttt
    30781 ccgcggcgac ctcgcggtca acggccagac cattgccgcc atcggcccgc tgacggcggt
    30841 gcgcggccgg gtcgagctcg acgccgacgg cctggcggtc gcacccggat tcatcaatat
    30901 gctgagctgg gccaaccgct cgctcatcga ggacggttgc tcgcaaagcg atattcgcca
    30961 gggcgtcacg ctcgaggtca tgggcgaagg ctcttccatg ggccccctca atccgaccat
    31021 gaaacggcag ctccgcgaac ggcaggggga catcaagtat gacatcgagt ggaccaccct
    31081 cggcgaatac ctggagcacc tcgtccggcg cggcgtctcc tgcaacgtgg cttccttcgt
    31141 cggggccacc accgtgcgca tccacgaaat cggctacacg gatcgccctc ccacgcccgc
    31201 cgaactggag cgcatgagac aactggtgcg cgaagccatg gccgagggcg cgctgggcgt
    31261 ggcgtcgtcc ctgatctatg ccccggcgtg ctatgccggg acggacgaac tcatcgcgct
    31321 ctgccaggtc gccgcggaat gcgacgggct atacatttcg catcttcgca gcgagagcaa
    31381 ccggctgctc gaagccgccg acgaactgat ccgcattgcc cgggagaccc cgatccggtc
    31441 tgaaatctat cacctcaaag tcgccggccg gcccaactgg cccaagctgg agccgctgat
    31501 ccggaagatc gaggccgcgc gggccgaggg gctggcgatc accgcggata tgtataacta
    31561 cgccgccgcg ggcacggggc tcgacgccgc catgccgccc tgggtgcagg agggggggct
    31621 ggaagcatgg gtgcagcgcc ttcgcgaccc cgccgtgcgc cagcgggtga agcgtgaaat
    31681 ggccgctccc gccgatacct gggagaactt ctatgtggcc gcggggtctc ccgaacggat
    31741 tgtgctcgct gggttcaaga gcgaccggct caagccgctc accggtctga ccctggccga
    31801 agtcgcggcg cggcgcggca cctcgccgga agaagccgcc atggacctcg tcgccgaaga
    31861 ccagggccgc gtggatgcca tctacttcat catgtccgag gacaacgtgc gccggcagct
    31921 caagctgccc tgggtgagct tcggttcgga cgaaggctca tttgcgccag aaggggtctt
    31981 cctcctgtcc aacccgcacc cgcgcgccta tggcaacgtc gcccgcctgc tgggcaaata
    32041 tgtccgcgac gaaaagctga tccccctcga agccgctatc caccgcctga gcgccctgcc
    32101 cgccgccaac ctcaagctcg atcgccgcgg cgcgctcaag cccggcttct tcgccgacat
    32161 cgtcgtcttc gaccccgccg ccatccagga ccacgcgacc ttcgcgcagc cgcagcaata
    32221 cgccaccggc gtgcgcgagg tattcgtcaa cggggtgcgg gtgctcaagg acggcgagca
    32281 taccggcgcc aaacccggcc aggtcgtccg cgggccggga tggcggcgcc cggcggcggg
    32341 ttgaaacggc gtggcgcgtg cggtcaggcg tgcgcgtaat cgtactgcgg cggccagaac
    32401 cggaaggggt cgggaaactt gcgctggttg ctgaccgaga aggcgccgta gccatccacg
    32461 tgggcgaaac tgccgcggac catttgcgcg tggagatggg ggaaccagtt gccgttgttg
    32521 gtggggccgc ccacgttgcc cagcagttgg ccggcgcgca cgcggtcgcc gacgcgcacc
    32581 caggtttttc ccaggtgcgc cagcagcagc gtcagccggg gccgcagctc cagcgcaatc
    32641 cgcccgcccc agccgccgga gctgtcctcg tcgtgccagg tttccaccac cagcgcgtcc
    32701 gccgggctat agacgcgcga ccgttcggcc acgttgaagt ccacccccag gtgaaaagcc
    32761 atgttcgggt gcatgtagtg cccctgccac agcgtggagc ggtcctcgaa ccagccgccg
    32821 taggaacagg tggcgcggta gcgccgatgc aggcggcgga cccagcgctg gcaatagtcc
    32881 ggctcgagca ggggattggg cttgcccggg cgctgctcgc ccagggccag ggcgcccagg
    32941 ggcagataga cccaccgccg cctccgatac tgtggaaaca gcagttgaaa catgtcaatc
    33001 tcccgggtcg ttcataaact tccgctcgat ggccttggga tggttgtcca ggttcaggga
    33061 catgtaaatg tggcggatgc cctggccgaa gccgaacttg cggaagaacg cgatggcatt
    33121 gcggttgccc tcgtaggtgt ccaccagcat gatgcggacg cccttttcga cgaaccgctc
    33181 ctgcagttgg cgggtgagcc gtttggccac gcgcttccgc ttgaaggccg ggtccacccc
    33241 cagccagtcc agccacccgt agagccacga gctgcccggc ttttccagca ggctccccag
    33301 ggcaaatccg atgacccgct cgtggcaggt cgccaccagg caggtttcct tgtccgccgt
    33361 gtaaatctgc aacacctcgg cctcttccca acagcggtac agggtggggg aggtttccgc
    33421 ggtgaacagc atgtttccca actccaccac ggcggggatg tcgtccactt gcatttctct
    33481 gatttcgatc gcaggtcggc ggggcatata tttattcgag acggttcaaa cagcttgtgt
    33541 ccagcataaa atccgttgac ggcgggaggc tattccgcgg ccggctcttc caggccggct
    33601 agaaaatccc tgaggcgggc gacgcattca tccacgccca gctcggcggt gggcagctcc
    33661 agttcgggat gctccggggg ttcgtagggc gcgctcacgc cggtgaagtc gctgatttcg
    33721 cccgcgcggg ccttggcgta aagccccttg gcgtcgcgct gctcgcagac cgccagcggc
    33781 gcgttcaggt aaacctcgac gaactggccc ggcggcaggc tgcgccggat ggcgtcccga
    33841 tcggcgcgat acggcgaaat gaacgccgtc acgcagatca cccccgcgtc ggcaaacagc
    33901 cgggccacct cgccaacccg gcgaatgttc tcgcggcggt ccgcgggcga aaaggccagg
    33961 tccgcgcaca gcccatgccg cacgttgtcg ccgtccagca cataaaccca ttggccgcgc
    34021 tcgaacagct cccgttccag ctcgatggcc agggtggtct tgcccgaacc ggagaggccc
    34081 gtcagccata cgacgcggcc cgcgtggccg tgggcccggg cccgctgcgc cgccgtcacc
    34141 cggcccaccg tccagtaaat gttcgggctc ttgtgcagcg aatcggccgt gcgccggggg
    34201 tagcggtcgt ccaggatcac tccgccgccc gccaggtcaa aaccatccac gatgacaaag
    34261 cggcccagcg cggcgatctc cgcgcggggg tcgaaggcga tcggcttgcg ggtgcgcagc
    34321 gttaattccg cggcttcatg gcggtggacg ccgggggcgc cggggcgttc gaggggttgc
    34381 agcgtggcgg catcaatgac gcgttcgatg tgctcgatct ggcacgcggc ctcctgcgtg
    34441 gcgagcttga ggcggtaagc gcggccgggc gcaaacggct gcttccccag ccagaagacg
    34501 cgcgcccgga agcgggtcag cgcgtacggc ggcgcgtcct ccggggcgcc caccgcgccg
    34561 cgctcgacaa agatttgttc ggtcagcgtc aggccgatgc tctcgcccgc ctcggccacc
    34621 gtccgcgccg ggccgttcca ccgttcgagg ctccggaccg tgctggtctt gtagccgggg
    34681 acaaagacca gccggtcgcc gaccttgagc cggccgctct cgatgcgccc cgccaggatg
    34741 cgccgctcgt caaatcggta cacgtcctgc accgggaacc gcaggggttg atcggccggc
    34801 ggggggacgg cctcgaactg gtcgagcgcc tccgccaccg tcgggccccg ccaccacggc
    34861 atgcgcgggc tcggcgcggc caggttgtcg ccatgccacg cggagatggg aaggaagagg
    34921 cggggctgca ggccgagcgc ctgaagccag gcgcggtact cggcctcgag ggcccgaaac
    34981 ggctcgggcg cgtagttcag caaatccatc ttgttgacca gcacgatgac ctggcggagg
    35041 cccaggaact cgagcagaaa gccgtgccgc cgcgtgtttt cccgcacccc ctcgcgggcg
    35101 tcaatcaaca tcaaggccgc ctcggcctgc gcggcgccgg tgaccatatt cttgaggaac
    35161 tcatggtgcc ccggcacgtc aatgatcacg tagcggcgcc ggggcgtccg gaaccaaatc
    35221 tgcgcggtat caatgctgat attctggtcg cgctccgcct ggagggcgtc catgaggttc
    35281 gcccactcaa agggaacccc gcgccgccgg gcggccagtt gaagctgctc cagccggcct
    35341 tcgggcagcg agccggtgtc atgcaacagc cggcccacca gggtggactt gccgtggtcc
    35401 acatggccga ggatgacaat ccggaattgt tcggcggcgt ccgggttcat ctacatatag
    35461 ccatcgcgcc gcagttgctc gaaggccgct tcgctctcct ggtcctgcgc ccgcccggcg
    35521 cgctccggcg tccgggtctg gcgcaactcc gcgacgatct ccgccaccgt ccgcgccgac
    35581 gacttgacgg ggaaggtgca gggataacag ccgatgctcc ggtagcgctc gccccgctcg
    35641 ttcgcgaagt agagggggat gaccggcacc ttctcgcgct cgatatattc ccagatgttc
    35701 agctccgtcc agtgcagcag cgggtggatg cgaatatgcg tccccggcgc gacgtccgtc
    35761 tggaattgat cccagaactc cggaggctga tcctcgacgt tccattccat ctgccggtcg
    35821 cgcgggctga agtagcgctc cttggcccgg gtcggctcct catcgcggcg gacgccgaca
    35881 atgacgccgg tgaagccgtg ctggtgcagg accgcctgga gggcgtcctt tttgagcgcg
    35941 ctgcagcact cgacgcgcgt ggcccgcccc tgcggatacg tgcgcccgcc cgccagcgcc
    36001 gcgtcgttgc atcccacaat gagctggagc ttccactcgc ggcaaagccg gtcgcgatac
    36061 tcgatcatgc tcggaatctt gtagcgggta tccacatgca ccagcggaaa cggcacgtgc
    36121 ccgaaaaagg ccttgcgcgc cagccacagc agcaccgtcg aatccttgcc gatgctccac
    36181 agcatggcca gccgctcgaa ctggctgaac gcctcgcgca ggaggtagat gctttggttt
    36241 tcgagttcgt ccagcagcat ggtctatggc tccaccgccg gcggccgccg cggcgccggg
    36301 tccggcggcg ccaaagctcc ccgcgcccgg ccggcgggac gcccgattgg ccggcgcaaa
    36361 gccgctgcgt ggcactggat ccgcggatca caatgccgac gggagagttg gaactcgcgc
    36421 atcaatcggt cgcctgaata cgcgggcaag atgcccggtc cgcccgcgtc gcgcaagccg
    36481 cgtgatgccg ggggccgcgg gcggccgggc ccgcgcccgc cccggtgccg cgcaacgcca
    36541 ccggcgggaa gcccggtggc gtcgccggac gatggttccg gatgaatcag tccagactca
    36601 ggacgcggta gaaccgctgc ggcgccccca ccgcatcctc cttcgaggcg gtgtccgcgc
    36661 tggcggtgat atccggcggc aggttgctcc agttcaggtc cgtaatgtta tccttgtatt
    36721 cgacgcgata gttcctgccg gcaatcgccg tccaggtcag cacgaccctg ccccccaccc
    36781 actcgacctt ttgaatgcgc agcggcgcgg ccaccgcgat gctaaaggtg gtgctgtcgc
    36841 tcaggctcgg aaggccgtcg tccgtggcca tgatggtgat ggccgggttg ctgcccgcat
    36901 cgccatccgt ggtcggccag gtgaagatgc cgctggcggc gccgatggtg gcgcccgccg
    36961 gcgcgcccgc gcccaggctg taggtcagcg tctggccggc atccgggtca tacgccgagg
    37021 ccgtcaccgt ggccgtggcg ccggcatgca ccgccaggtc gttgatggcc gacaggacgg
    37081 gcgcggtgtt aaccgccagg gtcgcatccg ggctgatcgt cgaaccggcg gcattgctca
    37141 cctcgaccga atagttcccg gcatccgcgc tctgcacatc cgggcgcgaa tagctgctgt
    37201 cggtcgcccc ggcgatggcg atctcgttga agcgccactg gtagctcaag ggcggcgtgc
    37261 cattggccac cacggagaac gttaccgtgc tgccggcggc caccgcctgg ctggcgggcg
    37321 gggtcacaat atccggcggg tcggccacga ccagccacgc cggcatgctg atcacacccc
    37381 ccaccgcgtt gctgacggcc accgtatagc tcccggcatc ggcctggctc acggtgttca
    37441 acgtgagctc cggcgtggtg gcgccggaga tgttgccgcc atccgccaaa tccacgccgc
    37501 ccttttgcca gcggtaggtg agcggaagct ggcccgcggc ggtcaccgta aagctggccg
    37561 tggcgccggc gctgttggtc tggctgagcg gctgcgccac gatcgccggc gcgacggaca
    37621 gggtggcggt ggcgctgacc accggcccgg cgccgttgct caccgtcacg ccgtagctgc
    37681 ccgcatcgct gtcctggaca ttgctgagca ccagcgccga ggtggtggcc ccggcaatgt
    37741 tggtgccgtt cttctgccac tggtaggcgg tgacggtgtt ggccgtctcc acaaagaagg
    37801 tggcggtgcc gccggcggcc accgtctggc tggccggctg cgtggtaatc tgcggcaaat
    37861 cgaaatactc gaccttcaca tcgtcaatcc acgagtcccc gagcacttcg ctgcccgtcc
    37921 ccacggagcc aatcgccgcc gaatccaggc tggagcgcgt ggtcccggtg atggtgcggc
    37981 tcagaatgcc gtccacgtag aagtccaccg tcgtgccatc cgcccggcgc tcgatgacga
    38041 atttatgcca gcccgccgag cgggccggcg cgcccggcgc gttcaggttg aaccagccgt
    38101 tgttggcgcc cgacaccacg cgcccctggt acttggtcgc gtcgtacgtc tcgccgggca
    38161 tcgtgaccgt attatatttc ccggcgcaga acagttggac cagcgagccc tggttgtagc
    38221 cgccgccggt ataaccgcgg acatccacga acgaccgggt ctgggtgctg tcgtaaatcc
    38281 aggccgtgat ccgcagccgc ccatccacgc tcacccccag gttgcgatac atgcgatcca
    38341 aggagctgtt cacataggcg ctccgggtcg ggctgacgga ttgcgccgtg gacaccgtca
    38401 gcgcggtggc cgggggcacg aagttcgtcc agttgatcgc cggcgactcg aaatcatcgt
    38461 agaacgcaat caccgcgttg accgccagcg cggcgttggc gctgaccgtc gcgccgaagc
    38521 cgttggtgac caccaccgag tagttgccca cctcggtctg ctgcacattg ctgatcgtca
    38581 gcgtggcggt ggtcgcgccg gagtatttgc cgccattgga aagattcacg gcgttcttgc
    38641 gccactggta ggtcagcgtc gggttgccgg tggccaccac cgtgaacgtc accgtggagc
    38701 cggggtcaac cgtccggctg gccggctggc tggtgatgga gggcgcgccg ctgacctgcg
    38761 tggtggcgct ggccgtgccg gagtaggccg agttgcccag gtggctgttg taggcgcgga
    38821 cgcggtagta gtaagtggtg ccgccgctca gcccggtgct ggcgtaggtc gtggcattgg
    38881 cggccgtggt cgcgatttcg gtccagggcc ccccgctggc cgtggcccgc tcgaccttgt
    38941 aactgtgctc cagcccggaa ttatcattcc acgacaggtt aatctggctg gcggaagcgg
    39001 cggtcgccgt cagcccgctg ggggcggcgg gtgtggtgac ccccacccgg tcgcacaccg
    39061 cgtagaagaa cgccgccgcc gcgttgtcct ggccggcggt gctgttaatc aggttgaact
    39121 cggcctggtt ggagacaaac aacatttccg ccaggcacga aggcggcgtg ggcgagcaca
    39181 tgacgaaatt gtagcgcttc accccgcggt tggcccgccc caggaactgg atcagccggt
    39241 tctgcacttt ggtcgccaaa tcctggtctt cggcggccac cccgttgcaa tagtccaggc
    39301 accagaacgt ctcggtgccg tgcgcgcccg ggtccgagaa agaattgcag tggatgcaca
    39361 atcccgcgtg cgggctttcg gcgttgacca ggtcgcggcg cgcgtagaga tccaccgcca
    39421 cgtcggtcga gcgggtcatc accacgctgc aacccgccgc ctgcagcagg gtgcgcagct
    39481 tgaggctgac cgccaggttg aaatcggctt cattcggctt gccgggcccg tcgtagccgg
    39541 tcgcgccggg gtcgctgccc ccgtggccgg gatcaatcac gactttgcgc ccgctcagct
    39601 ccagcgccgg cagcgaggcc gcgcccagga acaggagggc gaggatgaga gtaaggagag
    39661 gtttcaggct tgtgggtttc attgcttgtt gtggtttgtt tggggtctct ttgcggtttt
    39721 attgcagcca aagcagcagc acccgccccg cgtcttccgt gcggcaggcg atgcaccggc
    39781 catcgggcga ccaggccggc tcgcactcca gcacctcggg cgtgtgggtc aatcgcgtcg
    39841 gttcggtgcc gtcaacattg atcacataca aatcggaact caaaatctga tgcccgtcat
    39901 cccggctcac atcataaacc agcatcgtgc catccggcga ccacgacggg tgatgccccc
    39961 gcgccaattc ccgcttcttc gagccgtccg gcgccataat catgagcacc cccatctgcg
    40021 aataaacaac ctggctccga tccggcgacc acaccggctc catgcccccg tccgtggtca
    40081 gcgccgtccg gacgccgctg tccgggccgg cccgccagac ctgcccccgg tcggtgaaaa
    40141 ggagcggcgc cggctgggac gcgcgcgtcc cgaccttccg cttcgcgcgc tgccaggggg
    40201 tgttgacgag attggtgccc agcagaaagg aaacgcactg gttcccgttg gagctcagcc
    40261 agaccggcgg cgaaacctcg cccgccggct tgctcaactc ggccgttttc ccgctggcca
    40321 gatcaacgga tttaatgaca taccacgtca ggttgctttc cgtgcgccgg gtgcgatacg
    40381 cgatccgggc gctgtccggc gaccagtcaa accggtatcc cgaatccgtt tccagcgtca
    40441 gcagcttcca ctccttgcca tcccggcgcg ccagctcgat ccccgccatc ccggcgcggc
    40501 ccagccccag ccactggcca tccggcgacc acgccggacg cacgtacccc gcgcgctcgc
    40561 cggacaacag ctccaccctg gcaatctgca ccgccgacgc cctgcccgcc ggagccgggg
    40621 ccgccggggg ggccgccgcc accgtccagc cgccacccag ggccagcccg agcgccaggc
    40681 acggcttcaa taaaacggcc acacgccagg tggtggaagc gggcaacagc ataacggccg
    40741 cctttatagt cataacgcat attgctgtca agaaaatata agaacaattt atcgggcaaa
    40801 cgcgcctctt tttcccgcgc cgacctcggg agagtttccc gcccgcatat ttcccgcggc
    40861 gaaggaatcg gcctgctcgc agcactcccc gccctccgtg ccgcacctgc ccccacgcag
    40921 cgcacggagg ccgttcgtcc ggtggccggg aagaatatcc acgctcccgc ccggcttggc
    40981 tgcggccctt gctggcgctc cttcagacct gggcgctgcc catgagcctg gccggtcgtt
    41041 aattcacgtc gctatcttcg ttgaccgccg cctccggcct gatacccttt ttccggtggc
    41101 tggggacaaa ccgctgcccg gttaactgac ccgcgcgcgc gggccgccgg gccgctctcc
    41161 cggccttgga ttacaataac catggaagtt tctcgcttat gaaattactc gattcccccc
    41221 gctccggacg gctcggagct tatgtctatt acacgacgcc cttcggccaa tgttgccggg
    41281 ccttaaccat gccccgcgat gcccggtctc ccgcccaggc ccgcgcccgc gccagcttcg
    41341 cggcctgctc ccagg
//
LOCUS       DAOPQG010000018        40601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1293_length_40601_cov_14.037316, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000018 DAOPQG010000000
VERSION     DAOPQG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 40601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40601
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1293_length_40601_cov_14.037316"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..1125
                     /locus_tag="PKI07_03420"
     CDS             454..1125
                     /locus_tag="PKI07_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC50132.1"
                     /translation="MKKTLIAILVFASAVAGVNAQPMNHGGGMTNNAPSADLVKLLRQ
                     PKVQALNSAMRELWSAHMYWTLITVDAYYNDPKGLGAKLDRLLQNQKDIGAAIVPYYG
                     KAAGDQLAKLLTEHIQLAVPVLKAAKEDNKAELDKAVKNWYANAKEIGSFLATANPKN
                     WTAKETEGALEMHITHTIAYSVSILKGDYNQSFGGFEEALRHMLHLADILTEGIAKQF
                     PARFH"
     gene            complement(1290..1853)
                     /locus_tag="PKI07_03425"
     CDS             complement(1290..1853)
                     /locus_tag="PKI07_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOC50133.1"
                     /translation="MTLLQRAIQDSLAVTRALGALAKPLDRAVALVVDCLSRGHKLLL
                     CGNGGSATDASHVATEFLCRFREDRRPYPALSLTVNGEFMTAVCNDYHADEIFARQVW
                     GLGQKGDLLIAFTTSGKSRNILRALEEANRKKMKSICFLGRDGGFTKGVATLDLLAPG
                     DNTARIQEAHTLLFHVLCEAADQKLPK"
     gene            1959..3515
                     /locus_tag="PKI07_03430"
     CDS             1959..3515
                     /locus_tag="PKI07_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOC50134.1"
                     /translation="MNATDQIRKKLGTLPHQPGIYLMRDRFGTVIYVGKARDLRKRVS
                     QYFQPARRRGWDLKFKALVEAIHDFDVHVVRSDPEAVLLEGKLIKEFHPRYNVSFRDD
                     KQFLMLKVNLNDPIPRFTLTRLKQDDGARYFGPFPNSSALRTTLALVRRRYHLRGCRP
                     LTPTARDYRHCLYAHLRYCTAPCIGNVSSAEYRAQVRAACEFLEGQCAEMQEQLAAEM
                     RAAAAAQEYERAARLRDLLSDLQSTTRKLSRFPRVPYRLPRALDPEKDLAELARVLGL
                     PAPPPRIEGFDISNISGTFKVAALVSFRHGRPDRANYRRFQIKTVAGQDDFASVAEVV
                     RRRYTRVLKGLSAGAPPAARPGGPPPAARAAPAAAVLPDLILIDGGKGQLSAARAELE
                     KLGLGHIPLIGLAKEFEEIYRAGRPAPLRLPPESGALKLLQRVRDESHRFANTYNAQL
                     RLRKISESLLDEFPHIGQRRKAALLKKFGSVQRLRRASVEQIAEVPGFGGKTAAELKA
                     FLAARPGGGV"
     gene            3595..3999
                     /locus_tag="PKI07_03435"
     CDS             3595..3999
                     /locus_tag="PKI07_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50135.1"
                     /translation="MNIQVAVLCDAATDDNGKLNLLGAFDTIYTQQLPAVHPQCSIAL
                     RVTFASADEGRHQLQVNFVDADGRSIAQFPPIPVEVTLPDDMHFGTRNFIVNIQQLKF
                     DHPGLYAIDIALDGRPQASIPLLVRHTPPGPA"
     gene            complement(4036..4821)
                     /gene="nagB"
                     /locus_tag="PKI07_03440"
     CDS             complement(4036..4821)
                     /gene="nagB"
                     /locus_tag="PKI07_03440"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOC50136.1"
                     /translation="MEVIIQPNKEAAAALVARIVAHELRANPHLVLGLATGQTMECLY
                     RHLVRLHQTENLDFSLCRTFNLDEYVGLFPADPNSYRYYMNHHLFRHVNVDIRNTHLP
                     NGLATNLNEECLRYEATIKGFGGIDLQILGIGKAGHIGFNEPLSALHSRTRVKALTPT
                     TLKQNAGAFGGEDKMPRRAITMGVGTILECHRCVLLATGDSKARVIAQAVEGPITSMI
                     SATALQLHPRATVVVDEEAASELREKDYYRWIFDNEPEWEHLR"
     gene            complement(4925..5812)
                     /locus_tag="PKI07_03445"
     CDS             complement(4925..5812)
                     /locus_tag="PKI07_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50137.1"
                     /translation="MRSYTVTLAFEDYHSHRQVALVLRGDYFVAERVIRAPNRQTAVR
                     LALQWFAKYFKHHAGPPEKVLTVDDPAGEAAYRPGFNGDDLRNLFLPPDVRARLLAEA
                     RGELVHDAASGRLRYRDGAACQPPPEARGQVRVIAPCVCQNERGTLFYRITEQTQASR
                     SGQLLRKRKTRLIRLTARTLAEAAKEIEDRRLHELHQARRASRQRSLAVLQKLAAALG
                     PGPATAAAPGAGDSPPLPFPAVPPRRGPDSQPPPPNNSPSPAAAASPPDKPGVIIPLI
                     RPLDPDQTPSAPEPTAARR"
     gene            complement(6069..6359)
                     /locus_tag="PKI07_03450"
     CDS             complement(6069..6359)
                     /locus_tag="PKI07_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50138.1"
                     /translation="MSNTALLHRKPPSVKTLPEVGAGAPNPAACQVRALVPGFQGGWC
                     LVRPIPYCPHGFAYGDGYLCRHPEIERIIARTQAQQSGRAAGKTPPRAPAKR"
     gene            complement(6567..7019)
                     /locus_tag="PKI07_03455"
     CDS             complement(6567..7019)
                     /locus_tag="PKI07_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC50139.1"
                     /translation="MNNQSAARAARKRAGRQIRIVLVDDSAPARAMLTRLLAPYLQVC
                     IAGQAASGTEGLALADRLQPDLVITDLQMPGVNGLQLVERLRRQHPAMRSLVTSVYES
                     TTCRAACLRHGADAFIGKQRLPEEFPPLLARLFPEAAEPAASPCLESI"
     gene            7256..8668
                     /locus_tag="PKI07_03460"
     CDS             7256..8668
                     /locus_tag="PKI07_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50140.1"
                     /translation="MSPELNPPTTRREFLKDTGRWAALSALAGLTLPHVHAAADDTIR
                     LALIGCGGRGSGAVANAMSAGGLVLGDDGGTKRAALSGAGGPVKLVAMADLRRDKLDH
                     SYKSLSESLRDLIEVPPERRFLGFGAYRQAIDCLRPGDVAILATHAAFRAPHLEYAVA
                     KGVNVFMEKDFASDPGGIQRILKAGEAAEKKNLKIAAGLMCRHSSARQALIQQIRDGA
                     LGDIQLIRAYRMDSSYAMPPFPRGENELLWQLSPGHPYQFLWAGGGLFIELMIHQMDE
                     CFWIKDAWPVAAHGVGGRIPGSTDCSQNLDHYAVEFTFPDGAKAQVVNRCISNCYTDF
                     ATYIHGTRCAAQFSGNIHAPTTHIYKDQRTERSNLTWRAPRETVNPWQAEWDVLLSAI
                     RQNKPHNETRRAALANLGAIMGRAAVHMGKIVTWDEVMASNFQFCANVDALTEQSPAP
                     VQADAQGRYPAPIPGQTVEI"
     gene            complement(9362..10186)
                     /locus_tag="PKI07_03465"
     CDS             complement(9362..10186)
                     /locus_tag="PKI07_03465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC50141.1"
                     /translation="MTAPSLLLLIPAYNEELRLEPVLRDYARYFHDHYRGRFQLVVVL
                     NGCTDNTLGVVQRVAADYPAVSALDFPEPIGKGGALIEGLKLAPLADLIGYVDADGAT
                     PPRAFHELAQHMDGVDCVIGSRWLPGAVLHVEQSGRRRFASRAFHLIVQILFRMNLQD
                     TQCGAKVMRREAIQAVHSSLCIADMAFDINLLYSLHRAGYRIREIPTEWTDQIGSKVT
                     LARTSLVMFLSALRIWLIYSPRIYRLLRPLRPLEGWVYRKLRAPQPRPASPAQSPR"
     gene            complement(10240..10695)
                     /locus_tag="PKI07_03470"
     CDS             complement(10240..10695)
                     /locus_tag="PKI07_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOC50142.1"
                     /translation="MIPITRALSAEHRMFEALFDEIEASLSHLKHLADIKQLARLVEG
                     ILRQHARVEDDLLLLARGFSRTELRRFEQTRHEHQEIDGRLTRIRAAHALPEARTLLR
                     GALAASRRHFKNEERKVFPTVEKVMDPQALARLGAVWFLRRQTPARWSI"
     assembly_gap    11009..11108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11109..11300)
                     /locus_tag="PKI07_03475"
     CDS             complement(<11109..11300)
                     /locus_tag="PKI07_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50143.1"
                     /translation="MTSYSALLSWLDANKSFAARVGLTGRQPRVVTDNPTHSQTEKEK
                     TQGPSNPKPLARPRRLMIKP"
     gene            11282..>12704
                     /gene="pilM"
                     /locus_tag="PKI07_03480"
     CDS             11282..>12704
                     /gene="pilM"
                     /locus_tag="PKI07_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOC50144.1"
                     /translation="MLNTKSFLTVDFGAGSLKLAEFEVNEAGGLRLKQYGLKALGAEG
                     AQESTREAVILKALQEILAEQGIKARKINVSAPGFHVFSKFVKLPPVEAGKVTQMIQY
                     EAQQNVPFPLAEVVWDYQILGTTPGGELEVMLVAIKANVVEGLFRVTETAGLQLQLTD
                     VSPAALCNAFRYNYGDLEDCTMLLDIGAKTSNLLFFEKGKVFSRSINLGANSITQDFA
                     NEAKLKFDAAEKLKIDEGFVSLGGAYEEPDNPHQAAISKIARQFMTRLHIQVNQTMQF
                     YRGQQGGSAPQRLFLSGGASVMPYTAQFFAEKLNVPVEYFNPLRNIQLDPGLNLEELA
                     RVAHSLGEVVGLGLRNLARCPVELNLMPASTLRWQSFNQKKPYLLATVCSLVAVVAAM
                     GFLFDKLAAVKREVLANVEKDVAPLQQKEREFKSAYGEMQKTRAEVDRVVGWIQDRYY
                     WVDVFGELRQALMRVEQTTKSKLR"
     assembly_gap    12705..12804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12805..13062
                     /locus_tag="PKI07_03485"
     CDS             <12805..13062
                     /locus_tag="PKI07_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50145.1"
                     /translation="DAAVTDTNAVATLNLTFRAVSLKDVSGQADADKGIAYAMLHELQ
                     NSPLFDPDPQATRTASDVTHDEQTGTFTFSIIARLRHPLKL"
     gene            13066..14160
                     /locus_tag="PKI07_03490"
     CDS             13066..14160
                     /locus_tag="PKI07_03490"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HOC50146.1"
                     /translation="MDWIKRNLYFLVGSFVALVLMGLAGWYLYSKGQENAETLGKLEE
                     QYDKLKRLNEQRPHPGSDKVDNIKAAKEQQVELQACLQKARAHFQPCPRIPAPEATNV
                     TSQEFSSALSRTIDELQRAATKANVTLPGRESGSSAAYSFSFAAQRQRLAYAAGSPDL
                     LAVQLGEVKAICEVLFQANINSLESLRRERISEDDYKGPQTDYLPEHSETNELAVLTP
                     YEVVFHCFSSELAGVLAGFASSPYSLQVKTINVEPAPAVVAPATPAAPVMYTMPAVVP
                     PPAAPPPPPPPPPSMAAEAPPSMSFEERYGRAGRSLGPAPAPAPMQPRYVPPMPVAAA
                     PVSRGPTPALDERQLKVTLMLEVVKLLAPK"
     gene            14162..14965
                     /locus_tag="PKI07_03495"
     CDS             14162..14965
                     /locus_tag="PKI07_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50147.1"
                     /translation="MDFLKKHYEKVVLGVVLLGLAVAVAFLPLKIAGEKQKLDEMRTR
                     LISRNVQPLTNLDLSLADAALKRAAVPATVDFSAPNKLFNPMLWQKTPEGRLLKADAS
                     SVGPNAVVVTRQTPLYLKLALDEVTMTDSGLRCRIGIENEAALNPRDRKKQRYCRQGD
                     KNEVFAVREVRPMPNNPANATVALELNDGGPRVEISKDHPFQRVEGYVVDLKYGPENK
                     VWNARRMGALLTFGGEEYNIVDITEDEIVLLAKSNLKKWTIKHSAVAAP"
     gene            15003..17705
                     /locus_tag="PKI07_03500"
     CDS             15003..17705
                     /locus_tag="PKI07_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50148.1"
                     /translation="MIKAASLPLALILLAAAAPGAFAQLAPQDAAVNEAVYRQAHRIT
                     LRQKLAEAQAAQERRALASAAKLYDDSWELVQKIGTGVEAEREQAVAGLAAVRLELAR
                     AAQRRGDYREARTQVDDVLRVDPTHAAAVEFKRGNEKLLAGQRGTIPSEEVRSQVPAV
                     AEEKITASTLVHDGKLLYEMGKLDESEAKLKQALRIDPHSEAALYYLNLVSEAKYIRS
                     AKRHEVTMREDIRAVEQAWANPVSREKLLVPNPYAQTNLIHTGQGRQAIMIKLDRIRL
                     DSVKYDNLPLGEVIINLNDECKKRDPEKRGINFLIHQAGENASAGPQPMVLGPDGNPL
                     PAPMPEQVDMSSIAIKINPALTDVRLADALDAIVRVADHPIKYSIEDYAVVFSLKPRE
                     ATPEPLYIRTFKVDPNTFYQGLQGVGSYVFGESMNVGQNTGGGGFGGATGGGMTGGQN
                     QNQISGAIMARVRIAGGTVMGGRQGGGGMGGTETAGGLNYLTRTNDMSEVSRAVINFF
                     SSLGVDLDPSRNPGKALFFNDRQGVLIARATLQDLDMIEAAIQVLNTVPPQINIKSKI
                     IEISQSDTKALGFDWFLGNMLMNGGKIGGQPGTAPSFTGQPTPANPLGVFPGNPYASP
                     PTTIAPTATDGLLTSGLRNPSTTLFTLTGILTDPQFRLVIKALQQRQGAELLAQPEVT
                     TTSGRQAQMKAADVKSIITSYSFSQAQSTGVSTGTGTTVNQAQPTVVYPIPEQMELGP
                     VLDVIPCVLSDGYTINLTLIPTLTEFVGYDNPNEVGASTAINQSLAGGLIMVPTVLPH
                     FTVRQVVSTVNVWDGQTVVLGGLLSETVTTIKDQVPVLGDLPLVGRLFRSESKTSGKK
                     NLLIFVTPTLIDPAGNRLHTDDEMPFAQTGFPAQPPVVAGAGKN"
     gene            17783..19681
                     /locus_tag="PKI07_03505"
     CDS             17783..19681
                     /locus_tag="PKI07_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOC50149.1"
                     /translation="MSLPLFARSLVAGALLVFAAPVAVVGQTNLFQTNGVEYAIAGAL
                     TGDQVHPGLSLRSTGGYLVWEDNLTDGDGLGISALQLNSSLSGTYSPFRVNALGAGDQ
                     EFPQVCRLSGGGVAFAWQGGRRGSQNIYARFLSSGGTWVTPHSDVLVNTFTNNFQIAP
                     AMAPLANGNVVIAWGSYNQFSANSLQDVYAQVLAPSGQKVGGEWLVNQFTSYNQRTPS
                     VAALSDGRFVVAWVSEQQRFENSVDLYARIFNANGSPASAELAINSSTNVCANPHVVA
                     APNGGFIVAWSEKDLSSLWTNSWDVYARAFQHTGAAAGAAVRLNQQRLGDQYAPTLTL
                     NGVEMLAVWTSLGQDGSGAGVVGRFLRSDLTLSGDEFLVNTTTVGSQVHPVAAADGAD
                     RFLVVWGSFTGLASGFDLYAQRYARQVQSLAAPDPPFVTVLSSNALSVTWPPLAGLTV
                     AAYEVYADGAATPTAVVTNAWWTMTGLAPGSTHSFRLAYILTDGRASPISGATTNTTY
                     GTLTWGGIPYEWMVYYFGSDLYSWLLLYPPNADTDGDGASNLEEFLAGTSPINAASVL
                     RVKLHPTPQGPFLDWNTQPGLIYQVQASSNLKQWTNLGGVRFAPGTTDSMYVGGSAAG
                     YYRVLRVR"
     gene            19695..>20680
                     /locus_tag="PKI07_03510"
     CDS             19695..>20680
                     /locus_tag="PKI07_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50150.1"
                     /translation="MSRFFNIRQWAGWMFGLSLALVAPSAAAFSMLGPGESWQDITMG
                     YQKFHYITFPASGWAIHGNDFAWHPQNWGEEFRWNNKVLYYACDQSFLDYFGAEGVAA
                     VDAAVAVFNNLPNASDADLNQFPLEASRINYTAAALHLYDLKSTIMELLIERLGLIDP
                     ERWTWCIRARVLPPSLACPQFDFAVVQRNFDPVTGEPTRYVNGTLFTYGIQQICTPYD
                     FGDAVEFLVDPHADASARYSALATPKLTLPDETFYGWFHTGLTRDDMGGLRHLYRTNN
                     INLEGSGTNAVTYVVDTSRTNLLFMSNLTFLASQVITNDAATLQALFPGLSIA"
     assembly_gap    20681..20780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20781..21235
                     /locus_tag="PKI07_03515"
     CDS             <20781..21235
                     /locus_tag="PKI07_03515"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HOC50151.1"
                     /translation="FQFNKVGPIYLNGTYPLFVDEFGALLNFTWASFDASTNAPIIYP
                     SGTSLASLENQVLVQVSPPYLPDAVTGSAYSVQLYTSAATPNWQTPITWSLAAGSPAL
                     PPGLTLSPGGHISGTPGQMGFFNFIVVARDAAGRTVQQSYVINVAASP"
     gene            21276..24281
                     /locus_tag="PKI07_03520"
     CDS             21276..24281
                     /locus_tag="PKI07_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50152.1"
                     /translation="MKQKSILGLVALALTSAAWGQTYENWTVVQCPPEIPPNIVASTF
                     INHAEFRIDFTNFNMGSLPVTAPPYRGWSETVNFINDFGAHMSCNTGFNIETFPLSGS
                     GQRAASLYNYGTIDCGTLGTSNIFSIGGFLFNLLGSGEGVKCKVNATNIVSSGTINMG
                     FNSLLTLKGDRIDLSYGNLIMESGGFTGLGGGIFYQGGFFDGYWGKGDPVTQRPLYYP
                     NGIDPVSLYETVPPRTQYHIITNRNYAVIEQQLGGGTFNPYLLDVVDQSGSNRTVRAV
                     FLSNTNPAITANVYFPAFGGDPYQLGPDIIEWSWISTNFVGVTTNYLYLQDTFLSYTN
                     FQLLLNGYAGIGYNRPTYIPENYHFYQGNQLSLGVPAVSTVIPPGTFLTPQRNVTNEW
                     TAYQAIFQPASTIMGEVAGQNVTNLPGRIELTADKYLVLTNAHISSVNYLLLKATNHF
                     GGSAGAEITAPHADLYLRSTNGFLNITNVLAPYLPRPIGICDLFSARWTNIINNITNR
                     YHVLFVDTQLEPSSPLLVQTLSLSATNAATRDDRIVISDVFNVTSNLLLNTRRLTLAT
                     NAPGAPAPAGILNYLNPAVLWPSATPRLQYLTNHGGIRAQNLIVFGGSQSSPYIGPDS
                     STNPYAVFVNTGGVTNFGSFIFATNFLNSGTFLATGGTLQLRQARTAVLTNGAFLAAG
                     AAGAIRIDADSLLVSNHVLQAGNTLTLSVTNSLDDGSLADGADSVTHPNIWNVGYGIN
                     LLTRPARGGLLATTITNTGLPGAKVPNSWAGLDRGPVAAGFSNNGALGRLILDGLTNT
                     TRFEFTPVTGSNALYVDCLELRNHMTKLDGSGNVANLLFQPGMKIYYAQLIINGVSWA
                     EKLNGKNGGGLNWVPSYAGTYSSTNVVYPDGTTNRLNQALVQSCNLDSDGDGILNCQD
                     PTPVFVPSQVGLTAARAYLPQPAVVLSWNSIPYSTNWVFYKPTLQTAQWQLLTNFVLG
                     SAGGRQQIVQPSALNSSRFYRVRVDAAMP"
     gene            24380..34051
                     /locus_tag="PKI07_03525"
     CDS             24380..34051
                     /locus_tag="PKI07_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOC50153.1"
                     /translation="MRRLRMNAAVFNRLARGLLGLLTGLGLASSVFGDAPENDDFANA
                     TVISGIWGTVSGNTEEATAETDEPEHAGAAAANSVWYKLIPIQDGTLTIHTFGSAIDT
                     RLAVYSIPPNTNIAVNSSLFPVAASDDFDLRQDPFERPLRGPNGPYAEAGPSAVRFPV
                     KLGTAYYVVVDSNDGGGEIQLTWGYDFGGLFYFTKPQVEAGKTEGSIQFAVGRTFGCS
                     GRVLVDVVTTNYTGKNMPATEGVDYIPGQQTLIFDDYETLRTFRVPIIDAEFAGDPGD
                     PEDPEAPVSAPFNPNYHFNVQIVSVRFHDLENTNILAPPAILTGQDTSVGRMLDVNIP
                     RGAGDPGPDVAAFTNNIINFEVRRQVTTEYAGDGDGYVHVWFTRSGTADPNTTCSIKW
                     GINSAYSLGDLRNNLFDLSPESDYATPDPPSTPAPGVGPADFGPPSSGTVSWGSYDFV
                     PKEILIPINDDSLVEFNEDFLIELTNIDAQNCRMGEINKCTVTILFDYHDQPAGALEA
                     GFNAHTEPPNNPVPGTDAAIFAMAVQPDQHILIGGAFTTYNAFLRNRIARVNPNGTLD
                     RTFDPGNGADGLVTTLALQSDGYVLIGGDFLAVNGFNRYHVARLTPSGAVDTTFNPGA
                     GADGTVWSLAISTNDSVIIGGEFNSFNNYPSSRVARLDSSGNLDLAFDTSSHGINGTV
                     WAVAPLTDGKVIIGGDFNLVGGLLRPGIARLNADGSVDADFDPGLGANDTVYTLWVQN
                     DGNILVGGAFTQFHTASHHGLTRLLPDGQLDPAFNPGAGANDVVYSILVSPTNSAIYA
                     GGMFTTFNQTRRVGLVRLFPDGTVDTSFLDTAYNQFAGLTGPWFNPDRYPRSFVYALG
                     LEDVEVYGIARQDVLIAGSFNRVGGGGTNRRSYDTRENLALLVGGETVGPGNLTLTHP
                     TYSVNENGSFLWVTLTRTNGTLGSVGTRFSPDPFPVGAGAAVYGKDYTYNPLFYGLSV
                     YGSTWGPPGGPDTRMLSDGIWWRNYPDPRSVLPEIQQPGPFNNARVDILDNTIKDGNR
                     TFGLSLTIPWQLDTFFLGGANMPLATALGPQPSTTSLLLDNDNPPGTFSFITTNYTVS
                     ETAGRVSILVVRTNGTGGTVTLKYLTQNLVGSPPPGVGFARASTNSPTPNGNYYATNG
                     TLTFYPGITSRTFDVNIRQDGQINPDLALLLVITNIAAQNPASGTALGGITNAWLTII
                     DGDFPQGRLNFSSVAFMTNETAGNAVITVTRTGGSQGTMTVLCSTTNTLASATNTPAV
                     PNVNYLPVTNVLLTWANGDSAPKSFTVPVLHDNQITSNLTVGLKLSTPVLNGMTNYTA
                     LGPVSNAVLSILNVDSLGSLSFSSPHYSQNENGGFAIIPVVRQGGSVGTVIATFNAST
                     GINATPGIDFLPTNGTLVFGPGELSKVFTVQLLDNAIVDAPNRAVLLQLTNPVPPGIL
                     SSPATASLSLVDDETFNLPPGGMDPNFHPLFNGAVHAVALQPNGAIVAAGSFTLANYI
                     TRHRVARLNADGTLDFTFASKPGGADNTVRALALQTDGRMLIGGDFLRYDGINRSRFA
                     RLNLDGTLDWQFDPGSALNDSAYAIAETFADDSRSERKILVGGNFTLANGAPRRYLAQ
                     FNDNGSVDLAFKVAAGAGGLDGPIWAIAVQPDRKIIIGGDFRSVNGEPRNHIARLNPD
                     GSLDASFSNSGIGANSSVRALAVQRDGRILVGGLFTSVEDAPQNRIARLNSDGSLDAS
                     FNTGVGANDAVYAIALQPDNRILLGGAFTRCGDVARSRVTRLNSDGSVDPTINFGAGC
                     NNFVAAIAVQPDDAIVLGGGFTTYDNASAACLARIYGRSISGSGTFEFTSAIYGVDET
                     APNATITVRRRGGTSAPINGTNVYVTVAATDGPPPNGAVNDINYKSGLFTNTFPPGET
                     FATFLVPILRDFQVTPDLAVSLQLTSIDPLGLAGLGNQPIATLWVSNVDNAVRFSSPS
                     YTVAKNVQEGRATITIERFGSAVGAAQVDFMTTMDGTAPPYGRYLPVATNVVFAPGQQ
                     VQNVFIPIINDTQVLGNQTVTMVLTSPTNALLSTPSAATLTILETSTSPGTLAFSSAS
                     YGAGEGDAFAYLDVLRTGGSAGSVTVDYYTRDGTALAGVKYVATSGALVFADGETRKT
                     IAVPIINNASVELNQTFFVVLTNATGGAVLSGPTTVPVTIVDDDVGISFGAPIHAVSE
                     TAGTVSLSVLRQNGTNLTTTVHYATTNLTALAGTNYVGVSNGTLTFNPGETAKSLAVR
                     ILRDPRVTGDLQFMVNLRDPSAPAQLFNYSSTVVNVLDVDTGFTFGTTNRLVITNADF
                     STVTTASYGVLKSGSNLLVTVVRSNANTGTVSVNYATATNANDTAIAGVDYGATSGLL
                     TFSNNVSARSFTVPIFNHRQPEGDRTFSINLFNPSPGAQLIPPTAATVTITDDLAAIS
                     FSSSDYRVNENGGSATITVLRSNYTNSIVSVDYATTPLSAIPNVNYTNVSGTLTFNRG
                     ETVKTFRVPVMNDGILHGDTTVQLGLSNPVGNAVFINPNAATLTILETDGSLIVNAGA
                     SLIRESGPTNGVIDPGETVTLLFALRNQLGTNTASLVATLLATNGVSNPRANFSNSPA
                     VHNYGVLVPRGPSTFRPYSFTAAGTNGQPISATFQLQDGTTDLGQVVFNFVLGQSPIR
                     FGNSAAIVINDYSSATPYPSTINVSGLAGLLTRATVTLSNLSHSWPSDIDALLVSPTG
                     QKSFLMAKCGGSYPVSNVTLTFDDAAANSLTNGKSGPMISGTNRPTSYALATPPFPPA
                     LTPPPPYHTNLSVFNGKDPNGTWSLYVIDDGLGGEGVISNGWSLSLITAGVVPAAADV
                     GLAMAASPLTNIVNNNFTLTLALTNYGPARATNIVVTNLLPAGIQFVSGSQSAGSLTN
                     HAGLVAWSVPWLETNTWAVSVLTVRATNTGVFTNTAKVTTSTSDLNPDDDIASAIVAV
                     VAPTANLVLSLADSPSPVRLTNYLTYTMSVSNAGPATATGVVVSNTLPAGVSFISASP
                     GGARYATALTASGRLVVTFTNLGNLPPGARTNLSVTVRTEAAGTITDTAGCRSEIVDP
                     FKANNAAAVKTIVQAVPLPLVFAPRSGALAISWPAYAGYRLESTADLRPPVIWEPVTD
                     AHISLAGSTITAIVPIGPGNRFFRLNWSAAPAPLPLGISHTGANVTIAWPADLWNYNL
                     ESADTLSPPLVWTRVTSPASVLVNGLNTVTLPIGSSSQVFRLRAQQP"
     gene            34084..>34676
                     /locus_tag="PKI07_03530"
     CDS             34084..>34676
                     /locus_tag="PKI07_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC50154.1"
                     /translation="MKPSLDLARRFRGLPRLAWAALFLAFVQFAAGEEYNKTNLLVRF
                     DAEVQSAQRTNILNALGGATISQKYTLVPDLYLVTLPAGQQVDQVLSAYNATPGIRYA
                     EPNYVVRVAATTPNDPRFSELWGLHNTGQTGGTPGADIRAPEAWDFNTGSREIIVAVI
                     DTGVDYTHPDLTNNIWTNPGEIPGDGIDNDGNGYVDDV"
     assembly_gap    34677..34776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <34777..>38124
                     /locus_tag="PKI07_03535"
     CDS             <34777..>38124
                     /locus_tag="PKI07_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC50155.1"
                     /translation="FSCYGLTSVDLAAPGSDILSCQPGGGYQYMSGTSMATPHVSGAC
                     ALLLAANPNLTVAQVKQALLSTVDPVVPGLCVSGGRLNLSRALASLEPQGPALEYFTN
                     YVTGGNGNGIVEANECNTLDLVLSNPSMTNITGITVTLSTTNAGVAIPQPASAYPNIP
                     PGASATNLTPFRISTAPEFVCGTPIEFSLVVRSDVGVSVLPFTLATGAPGIPIRFDNN
                     SMVQIPAPGSTNSVIVVSNIALALNKVTVSLFIEEPYDYFLKLELIAPDGTTNLLTAN
                     NGYLGENYGLTCSPEALRTTFDDDALTPIGSGVPPFLGAYRPAKALAIFSGKSGTNVN
                     GTWQLRATDSGGFSHAVIHCWSLFLTPTICADGGGQCPGADMAIGIVGQPAVAIAENN
                     LTYSISVTNLGPSSATNVTVTHLLPPGAAFVSATASQGAWSQQGGVLTFTLGTMPTRT
                     QANLSAVVQPADLGTNYTCYSTATVTSGQPDFVPANNSATTRNQVTPPAADLAVGIAA
                     TPNPVLVGGTLTYTVSLSNNGPSPAPPITVTNVLPPSTALQSVAVSQGATTILGNVVL
                     WSLTNKLAMGASATATIKVTPTAEGVIKATATADAGSFDPVAANNSASVSTAVGPAAD
                     LALSLVGLPNPVVAGSNVTYKISVSNLGPSTATDITVSDLLPPEMIALSATATQGSGA
                     VSNHTLLWNLGTLSAGAEASITIVAATTTNGVFTSTASVLAAQADPNLTNNTASAVIT
                     VTAPFIDVVSAGATLTFESGPVNGALDSGETVTMTLRLRNAGNSSTRNLVATLLATNG
                     IVPVPPNQPQAYGILFPSGLAVGRPFSFAANGPAGGTVSATLLIQDGTNTHPPVSFAF
                     TLPSTQVVANTNSIIIPDPAAPNPPYEQQSGPAKPYPSIINVSNFTGTLGRVAVTLSN
                     LNHSYPADMSVLVVAPGGGQALLMSGAGDQPTVGGVNLTFDSSAPNALPEYGQLVSGS
                     YQPAAYGDAPSFPTNAPPGPYATALSALNSVNPNGAWSLYVYDQGSGDDGVIANGWSL
                     TLTRINPVNHLADLALTATVSPSPALVGGALTYIYTITNGGPNTAQTVAFTNVLPAGF
                     TLVSAGASQGTVFANPASVTVNLGNLGV"
     assembly_gap    38125..38224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <38225..39977
                     /locus_tag="PKI07_03540"
     CDS             <38225..39977
                     /locus_tag="PKI07_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50156.1"
                     /translation="LTNLVLITNLGPATAVSATLTQTLPAGAGFIASASSSTAGVITN
                     AGGTITCLLGNLASNATAAVSIVLTSSTAGFLTNTLTLGSGSYDPAPANNTSTRVATV
                     VDPAPQIINAGAVLTYESGPVNGAIDPNENITLSLALANIGTRVTGNLKATLLNSGGV
                     TAASGPQYYPALAPGGGALARTFSFKAAPALGSGLVATLQLRDERPGITNMLPNVVFT
                     FVAPATMNFTNAAAITIPDRGGGAPYPSVINVEGLTGRVSKATVTLKGFAHQFPHDVS
                     VLLVSPSGTNVLLMAHTGGGYPTNNLTLTFDDSAPGYLPNWDRLASGVYRPTFYEGAV
                     TLPGTISSQYYQHTLSALNWSHPNGPWSLYVFDDAIGDAGVIAGGWSLSLKTVVTVGN
                     VVNLAAGLLAPATLDLGAALTNTIRITNSGPDLATGVMLTNLLPANADLLEAAPPPSF
                     TNGNGGVVWNLGDLEPGAVAEFHTVVLPAQAGMATHIVTLGANEEDLNPADNTAQTTT
                     AVYYAGPAILSGFYAGGHFHLTVTAQRGFQYVVQGSTNLTHWVALHTNANPAGTFTFT
                     DTTTPALRSRFYRTLRR"
     gene            40006..40593
                     /gene="coaE"
                     /locus_tag="PKI07_03545"
     CDS             40006..40593
                     /gene="coaE"
                     /locus_tag="PKI07_03545"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502956.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOC50157.1"
                     /translation="MKVLGLTGGIGMGKSTAAQWLRDHGIPVVDTDDLARQVVEPGQP
                     ALAEVRKAFGADIVGPDGRLRRDALAQRVFADAADRARLESLLHPPIRALWHARMETW
                     RAAGHPLAVVVIPLLFETRAEPELDATLCVACTAATQHARLRARGWSPEQIAQRVRAQ
                     WPIEQKISRADYLVWTEAGLEVLAAQLERILQGLN"
BASE COUNT         7317 a        12521 c        12473 g         7790 t
ORIGIN      
        1 ttccaaccaa actcacccca atcagtcgaa tactccgccc acgatgaggg caacgtaagc
       61 agccgccttt cggcaacctc cgcccgggag ggggaatctc cccgccagct ttgaccccat
      121 cccgcccaaa aaccagggcg cgcgacaaca cccggccccc cgttccgcgt ttgggttaag
      181 gaggtggcaa ggcgggcggt tttggcaatc tcgcgcccac cgggcacgcc cgcagggcag
      241 cgttgctctc aggggcggcc gctgctttgg attgatgggc gctttcattc cggcccccag
      301 gttggctgtc cccggccaaa ccgctggatt ccgtggcccc cccctccttc gccccttaaa
      361 aaaagttgac tgccgggggg agactgtgca tagtattatt tataatagag acaagacgtc
      421 ctaaacaggt aataaacacg tatagttgaa agcatgaaaa aaacacttat cgcaatcctt
      481 gtattcgcga gcgccgttgc cggcgtaaac gcacaaccca tgaaccatgg cggggggatg
      541 acaaataatg ccccctccgc agacctggta aagctgctgc gccagcccaa agtgcaggcc
      601 ttgaacagcg ccatgagaga gctttggagc gcccacatgt attggacgct gattaccgtg
      661 gatgcctact ataacgatcc caaagggctg ggggcgaagc tggatcgcct gctgcaaaac
      721 caaaaagaca ttggcgcggc cattgtgcct tactacggga aagccgccgg cgaccaactg
      781 gccaagctgc taacggagca tatccagttg gccgtgccgg tcctgaaagc ggccaaggag
      841 gataacaagg ccgagctgga taaagcggtg aagaactggt acgccaacgc caaggagatt
      901 ggaagtttcc tggccaccgc caaccctaaa aactggacgg ccaaggaaac cgagggcgcc
      961 ctggagatgc acattacgca caccattgcc tattcggtca gcattctgaa gggcgattac
     1021 aaccagtcgt tcggcggttt tgaagaagcc ctgcgtcaca tgctgcacct ggcagatatt
     1081 ctgaccgaag gcatcgccaa gcagttcccg gcccggttcc actaattccg gaaacccgtc
     1141 cgggtgggtg atttgcgcgc ccgcctttgc gtcgggcttg cggggtgggc gcgcgcttcc
     1201 ggtttcgcgg ggcgccgggc gggcggactc ccggccgtgg cgccggggtc cgcttccggg
     1261 cggcggggtt tcgctcccgg gcgcggggcc tacttgggga gcttctggtc ggcggcctcg
     1321 cagaggacgt ggaagaggag ggtgtgggct tcctggatgc gggcggtatt gtcgccgggg
     1381 gccagcaggt cgagcgtggc gacgcctttg gtgaagccgc cgtcgcggcc caggaagcag
     1441 atgctcttca tcttcttgcg gttggcctct tcgagcgcgc gcaggatgtt gcgggatttg
     1501 ccgctggtgg tgaaggcgat gaggaggtcg cccttttggc cgaggcccca gacctggcgg
     1561 gcgaagattt cgtcggcgtg gtagtcgttg cagacggcgg tcatgaactc gccgttgacg
     1621 gtgagcgaga gggcggggta ggggcggcgg tcctcgcgga agcggcagag gaactcggtg
     1681 gccacgtggg aggcgtcggt ggcgctgccg ccattgccgc acagcagcag tttatgcccg
     1741 cgggacaggc agtccaccac cagggccacc gcccggtcga ggggtttggc cagcgcgccc
     1801 agggcgcggg tgacggcgag ggaatcctga attgcgcgtt gcagcagtgt catgccggcc
     1861 aggatagcgg aaggcggggg aatgggaaac ggaaaatgcg gcgggcggcg cgattgggag
     1921 tcgcgcgcgg gggcggcggg ttgtaagttg tcggcggcat gaacgccacc gaccagatac
     1981 ggaagaagct cgggacattg ccccaccagc cgggcattta cctgatgcgg gaccgcttcg
     2041 ggacggtcat ctatgtgggc aaggcgcggg atttgcgcaa gcgggtgagc cagtatttcc
     2101 agcccgcgcg gcggcgggga tgggatttga agttcaaggc gctggtcgag gcgattcacg
     2161 atttcgatgt gcacgtggtg cgcagcgatc cggaggcggt gctgctggag ggcaagctca
     2221 tcaaggagtt tcacccgcgc tacaacgtca gtttccgcga cgacaagcag ttcctgatgc
     2281 tcaaggtcaa cctgaacgac ccgatcccgc ggtttacgct gacgcgcctg aagcaggacg
     2341 acggcgcgcg ctattttggg ccgttcccga actcgagcgc gctgcgcacc acgctggcgc
     2401 tggtgcggcg gcggtatcac ctgcgcggct gccgcccgct cacccccacc gcgcgggatt
     2461 accggcattg cctctacgcc cacctgcgct actgcaccgc gccgtgcatc ggcaatgtgt
     2521 cgtccgcgga ataccgggcg caggtgcggg cggcgtgcga gttcctcgag ggccagtgcg
     2581 cggagatgca ggagcaactg gcggcggaga tgcgggcggc ggcggcggcg caggaatacg
     2641 agcgggcggc ccggttgcgg gacctgctgt cggatttgca gagcacgacc cggaagctga
     2701 gccgcttccc gcgcgtgccc taccgcctgc cgcgcgcgct ggatccggag aaggacctgg
     2761 ccgagctggc gcgggtgctc ggcctgcccg caccgccgcc gcgcatcgag gggtttgata
     2821 tttccaacat cagcggcacc tttaaggtgg cggcgctggt cagcttccgc cacggccgcc
     2881 cggaccgggc caactaccgg cgtttccaga tcaagaccgt ggccgggcag gatgatttcg
     2941 cctcggtggc cgaggtggtg cgccgacgct acacgcgggt gctgaagggg ctgtccgccg
     3001 gcgccccgcc cgccgcccgc cccggcggcc cgccgcccgc ggcccgcgcc gcccccgccg
     3061 ccgccgtgct cccggatctc attctcattg atggcggcaa agggcagttg agcgcggcgc
     3121 gggcggagct ggagaagctg gggctggggc acattccgct gatcgggctg gccaaggagt
     3181 tcgaggagat ttaccgggcc ggccggcccg cgccgctccg gctgccgccg gaaagcggcg
     3241 cgctgaagct tttgcagcgc gtgcgggacg agtcgcaccg tttcgccaac acctacaatg
     3301 cgcaactgcg gctgcgcaag atttcggaga gccttctgga tgagtttccc cacatcggcc
     3361 agcggcgcaa ggcggccttg ctaaagaagt tcggctccgt gcagcgcctc cggcgggcga
     3421 gcgtcgagca gattgcggag gtgcccgggt tcggcggcaa aaccgcggcg gagttgaagg
     3481 cgtttctggc cgcgcgcccg ggcggcgggg tttgaggcgg cggcggccgt ggaggctccg
     3541 ccggcggatt taaagcgtgc cccggcggcc ggggtgcgct ttaatcgggg ccggatgaat
     3601 attcaagtgg cggttttgtg cgatgcggcg acggatgaca acggcaagct caatttgctg
     3661 ggggcgtttg atacgatcta cacgcagcag ctcccggcgg ttcatccgca atgctcgatt
     3721 gcgctgcggg tgacgtttgc cagcgcggac gaggggcggc accagttgca ggtgaacttc
     3781 gtggacgccg acggccgctc catcgcgcag tttccgccca tcccggtgga ggtgacgctc
     3841 ccggacgata tgcattttgg cacgcgcaac ttcatcgtga acattcagca gttgaagttc
     3901 gaccaccccg ggttgtatgc cattgacatc gccctggacg gacggccgca ggcgagcatc
     3961 ccgctcctgg tgcggcacac gccgccgggg ccggcgtgac cggtccgggc gcccgcgggc
     4021 gggcggcgcg gcgcctcagc gcaggtgctc ccactcgggt tcgttgtcga aaatccagcg
     4081 gtagtaatcc ttttcgcgga gctcgctggc ggcttcctca tccaccacca cggtggcgcg
     4141 gggatgcaac tggagggcgg tggcggaaat catgctggtg atcgggcctt cgacggcctg
     4201 ggcgatgacc cgggccttgc tgtcgcccgt ggccaggagg acgcagcggt ggcattcgag
     4261 gatggtgccg acgcccatgg tgatggcgcg ccggggcatc ttatcctcgc cgccgaaggc
     4321 cccggcgttt tgtttgagcg tggtgggggt gagggccttg acgcgggtgc gggaatggag
     4381 cgcggacagg ggctcgttga agccgatgtg cccggctttg ccgatgccca ggatctgcag
     4441 gtcaatgccg ccgaatccct tgatcgtcgc ctcatagcgg agacattcct cgttgaggtt
     4501 ggtggcgagg ccgttgggca gatgggtgtt ccgaatatcc acgttcacat ggcggaagag
     4561 gtgatgattc atgtagtagc ggtaggaatt cgggtccgcc gggaacagcc ccacatactc
     4621 gtccaggtta aaggtgcggc agagggagaa atccaggttc tcggtttggt gcaggcgcac
     4681 caggtggcgg tagaggcatt ccatcgtctg gccggtggcc agccccagaa ccaggtgcgg
     4741 gttggcgcgc agttcgtggg cgacaatgcg ggcgaccagg gcggcggcgg cttccttatt
     4801 cggttgtata atgacttcca tcgcccggat gctgccacaa acggaagaaa aaagacaccc
     4861 ctaaaatgca gccccgcttt ccgcctctcg ccgccgggca atcccctccg ccgcggccgg
     4921 ggaactaacg ccgcgccgcg gtgggctcgg gcgcggaggg cgtctgatcc gggtccagcg
     4981 ggcggatgag cggaatgatg acgcccggct tgtccggcgg cgacgccgcc gcggcggggg
     5041 acgggctgtt attcggcggc gggggctggg aatccgggcc gcgccgaggg ggtaccgcgg
     5101 ggaacggcag cggcggggaa tcgcccgcgc cgggagcggc ggcggttgcg gggccgggcc
     5161 cgagggccgc ggccagcttc tggagcacgg ccagggagcg ttgccggctc gcgcggcgcg
     5221 cctggtggag ttcatgcagc cggcggtctt caatctcttt ggccgcttcg gccaaagtcc
     5281 gggccgtcag ccggatcagc cgggtcttgc gcttgcgcaa taactggccc gagcgggagg
     5341 cctgggtttg ctcggtaatg cggtagaaca gcgttccccg ctcattctgg cagacgcagg
     5401 gggcgatgac ccgaacctgg ccgcgcgctt ccggtggcgg ctggcaggcc gcgccgtccc
     5461 ggtaccggag ccggcccgac gccgcgtcat gaaccagctc gccccgggct tcggcgagca
     5521 gccgtgcccg gacatccggc ggaagaaaca ggttccggag atcgtcgccg ttgaagcccg
     5581 gacggtaggc tgcttccccg gcggggtcgt ccacagtgag aaccttttcc ggcggcccgg
     5641 cgtggtgttt aaagtatttg gcaaaccact gcagcgccag gcgcacggcc gtctggcggt
     5701 tgggggcccg gattacccgt tccgcgacaa agtaatcacc ccgcaggacc agggccacct
     5761 gccgatggct gtggtagtcc tcgaacgcaa gggtgacggt gtatgatctc atgcgcttcc
     5821 ccgggtgcgg gcgatgattc gatcaacttc cggatggcga cagaaatagc cgccggcgaa
     5881 atagaagccg aacgcgcatt ggggaaccgg ccggacgagg catcgcgcca ggttgggacc
     5941 ataccggttc cgctccgccc ggcattttgc cggaatgggc gcgccggcgc cgaccgcgcg
     6001 cgcgggatcc ggcggcgtgg acggcggaag gggggggtgg gaggatgaca tgggccggga
     6061 cactcgattc aacgtttggc gggagcgcgg gggggagttt ttcccgcggc acgaccggac
     6121 tgctgcgcct gcgtgcgcgc aataatgcgt tcgatttccg ggtgccggca caggtagccg
     6181 tcgccgtagg caaagccgtg cgggcagtag gggatgggcc gtaccagaca ccagccgcct
     6241 tgaaagccgg gaaccagcgc ccggacctgg cacgccgcgg ggttcggcgc gccggcgccc
     6301 acttcaggga gggttttaac cgaaggaggt ttgcgatgta agagtgcagt atttgacata
     6361 aagagtttgg ggagccggcg tgggggccgg cggatgggat ggtgggattg gtttgagagc
     6421 atcgccgggc aacagccggc ccggcgggca tgcccgggag gccgagcccg tccgcggccg
     6481 ccgctcgcgg cggatgggaa cttcccaggc gctgatttcc tcccggatcg ggaggcgcat
     6541 gctagagatg ggtttggccg gtgcattcat attgactcca ggcaaggtga ggctgccggt
     6601 tcggcggcct cgggaaacag gcgcgccagc aggggcggaa actcctccgg cagccgttgt
     6661 ttgccgatga aggcgtccgc cccgtgccgc aggcaggcgg cgcggcaggt cgtgctctca
     6721 taaaccgagg tgaccagcga gcgcatcgcc gggtgctgcc gccggagccg ctcgaccagt
     6781 tgaaggccgt tgacgcccgg catttgcaga tcggtgatga cgaggtcggg ctgcagccgg
     6841 tcggccaggg ccagcccctc ggttccgctg gccgcctgcc ccgcaatgca aacctgcagg
     6901 tagggagcca gcaggcgggt caacatggcg cgggccggcg ccgaatcatc caccagcaca
     6961 atgcggatct ggcgtcccgc ccgtttgcgg gcggcgcgcg ctgccgattg attattcatg
     7021 attatgccat agcatgcggc cgccgcgcca gaaggggtat ccggtgaatc ctgtattttg
     7081 cggggggaaa atcctgtaag gaaattccct accgggcggc catctttgga gttggcgcgg
     7141 gccggcggac gcggtagtgt tgcccgagcg ctgccccgcc agcatggcgg gccggttcgt
     7201 tttccgccgg cccggcggcg gaaccggaaa agtgactatt cgagccaaaa catttatgag
     7261 ccctgaactg aatcccccca cgacccgacg cgagttcctg aaagacaccg gccgctgggc
     7321 cgccctgtcg gccctggccg gcctgaccct gccgcacgtt cacgccgccg cggatgacac
     7381 catccgcctg gccttgatcg gctgcggcgg ccggggcagc ggcgcggtgg ccaacgccat
     7441 gtccgccggc ggcctggtcc tgggcgacga cggcggcacg aagcgggccg ccctgtccgg
     7501 cgcgggcggg ccggtcaaac tggtcgccat ggccgacctg cgccgggaca aactggacca
     7561 ctcctataaa tccctgagcg aaagcttgag ggacttgatc gaagtgccgc cggagcggcg
     7621 cttcctgggc tttggcgcgt atcgccaggc cattgactgc ctgcgccccg gcgacgtggc
     7681 catcctggcc acccacgccg ccttccgcgc gccgcacctg gaatacgccg tcgccaaagg
     7741 cgtcaacgtg ttcatggaaa aagacttcgc ctccgacccc ggcggcatcc agcgcatcct
     7801 gaaagccggc gaagccgcgg aaaagaaaaa cctcaagatc gccgccgggt tgatgtgccg
     7861 ccattcctcg gcccggcagg cgctcatcca gcaaatccgc gacggcgcgc tgggcgacat
     7921 ccagctcatc cgggcctatc gcatggattc cagctacgcc atgccgccct tcccgcgcgg
     7981 ggaaaacgag ttgctctggc agctcagccc cggccacccc taccagttcc tgtgggcggg
     8041 cggcggcctg ttcatcgaac tgatgattca ccagatggat gagtgcttct ggatcaagga
     8101 tgcctggccc gtcgccgccc acggcgtcgg cggccgcatc cccggcagca ccgattgcag
     8161 ccagaacctc gaccactacg ccgtggagtt caccttcccc gacggcgcca aagcccaggt
     8221 cgtcaaccgg tgcatctcca attgctacac cgacttcgcc acctatatcc acggcacccg
     8281 gtgcgccgcc cagttctccg gcaacatcca cgcccccacc acccacatct ataaagacca
     8341 gcgcacggaa cgctccaacc tgacctggcg tgccccccgg gaaaccgtca acccctggca
     8401 agccgaatgg gacgtgctgc tcagcgccat ccggcaaaac aaaccgcaca acgaaacccg
     8461 ccgcgcggcg ctggccaacc ttggcgccat catgggccgc gccgcggttc acatgggcaa
     8521 aatcgtcacc tgggacgaag tcatggcctc caacttccaa ttctgcgcca acgtggacgc
     8581 gctgacggag caaagccccg cccccgtcca ggccgacgcc cagggccgct accccgcgcc
     8641 catccccggc caaacggtgg aaatttagcg cagaactccg gcttgccagt ccgcccctgg
     8701 gccattagcc cccggcgctg ccctgcagaa agcacagagc atgggcgagg ctccgtgggc
     8761 gttcgggaaa cagcctccgg gccgcgcccc cagcgaatcc agcccccggg ggagtgccgg
     8821 attttcgggc gtaatcctca tcgataagcg aatccaacgg cctgaccgtc ggaccttgag
     8881 cgcctttctc ctgtaaaatc aacgatttac gcacgccggg aagggtaaat tgggggcgga
     8941 atccaaacca agccggctca gacgcacctt ggatttaccc cagacatacc ctgccttcag
     9001 gccgtgtaca aggggtgtac aagggatgcc caccgggagt ccatgagatt ttagctggta
     9061 cacccgatgg gcatcccttg tacacccctt gtacacggcc tgaaggcagg gtatatccac
     9121 ggtatctgga tagtggatgc ggatgcctgg aaaggccgga acgaagagag ggaacgggag
     9181 gattattact aagttccgta gaaacagcca gttatgaagc aaaggctggc aaagagcggg
     9241 ttttcaaaag cgggtgggcg tcctgggggc ttttagcttt acggatgccg tttgcttgta
     9301 aaatcaggga cttacgtgcg caatggagtc ggaatggggg tggaagcgga actgatccgg
     9361 gttagcgggg ggactgggcg ggggaggccg gacggggttg gggggcgcgg agtttgcggt
     9421 acacccagcc ttccagcggg cgcagggggc gcagcaggcg gtagattcgc ggcgaataga
     9481 tgagccagat tcgcagggcg gagaggaaca tgaccagcga ggtgcgcgcc agggtgactt
     9541 tggagccgat ttggtcggtc cattcggtgg ggatttcgcg gatgcgatag ccggcgcgat
     9601 ggagagagta gagcaggttg atgtcaaagg ccatgtcggc gatgcacagc gaggaatgga
     9661 cggcctggat ggcttcgcgg cgcatgactt tggcgccgca ttgggtgtct tggaggttca
     9721 tgcggaacag gatctgcacg atgaggtgaa aggcccggct ggcgaagcgg cgccggccgc
     9781 tctgctccac gtgcagcacg gcgcccggca gccagcgcga gccgatgacg cagtccacgc
     9841 cgtccatgtg ttgggccagt tcgtggaagg cgcgcggggg ggtggcgccg tcggcgtcca
     9901 cgtagccgat gaggtcggcc agcggcgcca gtttgagccc ttcaatcagg gcgccgcctt
     9961 tgccgatggg ctcggggaaa tcgagggcgg agacggcggg gtaatccgcg gcgacgcgct
    10021 ggaccacgcc gagggtgttg tcggtgcagc cgttgagcac gacgaccagt tggaagcggc
    10081 cgcggtagtg gtcgtggaaa tagcgcgcgt agtcccgcag cacgggttcg aggcgcaact
    10141 cctcattgta ggccgggatc aggagcagca ggctgggcgc ggtcacatgg cgtatttagc
    10201 agagccgcgc caaaaggcaa cggcagaatg cgccgggggt cagattgacc agcgcgccgg
    10261 ggtctggcgg cgcaggaacc agacggcgcc cagccgggcc agggcctggg ggtccatgac
    10321 tttttccacg gtggggaaga ctttgcgttc ttcgtttttg aagtgccggc gggaggcggc
    10381 cagggcgccc cggagcaggg tgcgggcttc gggcagggcg tgggcggcgc ggatgcgggt
    10441 gaggcggccg tcaatttcct ggtgttcgtg gcgggtttgc tcgaagcggc gcagttccgt
    10501 gcgggaaaag ccgcgggcca gcagcagcaa atcgtcttcc acccgggcgt gttggcgcag
    10561 gattccttcc accaggcgcg ccagttgttt tatgtcagcc agatgcttga ggtggctcaa
    10621 cgacgcttcg atttcgtcaa acagagcctc gaacatccgg tgttcggcgc ttaaagcccg
    10681 cgtgattggt atcatgagga ttagtgtatc cgaggccggg ggaagcgcaa ctgcgcattc
    10741 ggcggcgggg gcacgggccc ccggccgggg cggaatgaga gcggcgggaa gcgggcgcgg
    10801 gcgcgggccg gaagcccggc ggcggaggag cgcggcgggc ggcgggaggg tgcgcgggag
    10861 ccgcgccttt tgcgtgaagt tcacacgcgc gatttgggga agggggcgga aatcaaggga
    10921 aaagcggctt ggcatggttt gtggtaaggc gcatggttta atcatgagac ggcgcggccg
    10981 ggctaggggc ttagggttgg acggccctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnntg gtttaatcat gagacggcgc ggccgggcta ggggcttagg gttggacggc
    11161 ccttgggttt tttctttttc tgtttgactg tgggtggggt tatcggtaac cacccttggc
    11221 tgcctgccgg ttaacccaac ccgggcggca aaagatttgt tggcatcaag ccacgacagc
    11281 aatgctgaat acgaagtcat ttttaacggt tgattttggc gcgggcagtc tcaagctggc
    11341 cgagttcgag gtcaacgagg cgggcgggtt gcggctgaag caatacggcc tcaaggcgct
    11401 gggcgcggag ggggcgcagg aaagcactcg cgaggcggtg attctcaagg cgttgcagga
    11461 gatcctggcc gagcagggga tcaaggcccg gaagatcaat gtcagcgccc cggggttcca
    11521 cgtgttttcc aagttcgtca agctgccgcc ggtggaggcg ggcaaggtga cgcagatgat
    11581 ccagtatgag gcgcagcaga atgtgccgtt tccgctggcc gaggtggtgt gggattacca
    11641 gattctcggg acgacgcccg ggggggagct ggaggtgatg ctggtggcca tcaaggcgaa
    11701 cgtggtggag gggttgttcc gggtgacgga gacggcgggg ctgcagttgc agttgacgga
    11761 tgtttcgccg gcggcgctgt gcaatgcgtt tcgctataat tatggcgacc tggaggattg
    11821 cacgatgctg ctggatattg gcgccaagac cagcaacctg ctgttttttg agaagggcaa
    11881 ggttttctcg cgcagcatca acctgggggc gaactccatc acgcaggatt ttgccaatga
    11941 ggcgaagctg aagtttgacg cggcggagaa gctcaagatt gacgaggggt ttgtgagcct
    12001 gggcggggcg tatgaggagc cggataaccc gcaccaggcg gcgatctcca agattgcccg
    12061 gcagtttatg acgcggctgc atatccaggt caaccagacc atgcagtttt accgcggcca
    12121 gcagggcggc tcggcgccgc agcggctgtt tttgtcgggc ggggcgtcgg tgatgccgta
    12181 tacggcgcag tttttcgccg agaagctgaa tgtgccggtg gagtatttca acccgctgcg
    12241 caacatccag ttggatccgg ggctcaacct cgaggaactg gcgcgggtgg cgcattcgct
    12301 gggcgaggtg gtggggctgg ggctgcgcaa cctggcgcgc tgcccggtgg agctgaattt
    12361 gatgccggcg agcacgctgc gctggcagag tttcaaccag aagaagccgt atttgctggc
    12421 gacggtgtgc agcctggtgg cggtggtggc ggcgatgggg ttcctgtttg acaaactggc
    12481 cgccgtgaag cgggaggtgc tggccaacgt ggaaaaggat gtcgcgccgc tgcagcagaa
    12541 ggaaagggag ttcaagtcgg cctacggcga aatgcagaag acgcgggcgg aggtggaccg
    12601 ggtggtgggc tggattcagg accggtatta ctgggtggat gtgtttggcg aattgcggca
    12661 ggccctgatg cgggtggagc agacgaccaa gagcaagctg cgcannnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnngatgcc gccgttacgg ataccaatgc ggtcgcgacc
    12841 ctcaacctta ctttccgcgc ggtgagtttg aaggacgttt cggggcaggc ggatgcggac
    12901 aagggcatcg cttacgccat gctgcatgaa ttgcagaaca gccccctgtt tgatccggat
    12961 ccgcaggcga cccggacggc cagtgatgtg acccacgatg agcagacggg cacgttcacc
    13021 ttcagcatta tcgcccggct ccggcacccg ttaaagctct aggttatgga ttggatcaag
    13081 cgcaatttgt attttctggt ggggagcttc gtggcgctgg tgctgatggg gctggccggc
    13141 tggtatcttt attccaaagg gcaggagaat gccgagacgc tggggaagct cgaggaacag
    13201 tacgacaagc tgaagcggtt gaatgagcaa aggccccatc ccggctccga caaggtggat
    13261 aacatcaagg ccgccaaaga gcagcaggtg gagctgcagg cctgtttgca gaaggcccgc
    13321 gcgcatttcc agccgtgccc ccgcattccc gcgcccgagg cgacgaacgt caccagccag
    13381 gaattcagct cggcgctcag ccggacgatt gacgagctgc agcgcgccgc caccaaggcc
    13441 aacgtgacgc tgccggggcg ggagagcggc agttccgcgg cgtatagctt ttcgtttgcg
    13501 gcgcaaaggc agcggctggc ctatgccgcg ggcagcccgg acctgctggc ggtgcagttg
    13561 ggcgaggtga aggccatttg cgaggtgctg ttccaggcga acatcaattc gctggaaagc
    13621 ctgcggcgcg agcggatttc ggaggacgat tacaaggggc cgcagacgga ttatttgccg
    13681 gagcattccg agacgaatga gctggcggtg ctcacgccct acgaggtggt gtttcactgt
    13741 ttcagctcgg agctggccgg ggtgctggcc ggctttgcca gttcgcccta cagcttgcag
    13801 gtgaagacga ttaacgtcga gccggcgccg gcggtggtgg cgcccgccac gccggcggcg
    13861 ccagtgatgt ataccatgcc ggcggtggtg ccgccgcccg ccgcgccgcc gccgcccccg
    13921 ccgcccccgc cttcgatggc ggccgaggcg ccgccgtcca tgtcctttga agaacgttac
    13981 gggcgcgcgg gccgttcgct gggaccggct cccgcccccg ccccgatgca gccgcgctac
    14041 gtgccgccga tgccggtggc cgccgcgccc gtcagccggg ggccgacgcc ggccctggat
    14101 gagcggcaat taaaagtcac cctgatgctg gaggtggtga aactgctggc ccccaagtaa
    14161 aatggatttc ctgaagaaac attacgagaa ggtcgtgctg ggcgtggtgc tgctgggcct
    14221 ggcggtggcg gtggcctttc tgccgctcaa gatagccggg gaaaagcaga aactcgatga
    14281 gatgcgcacc cggctgattt cgcgcaacgt ccagccgctg accaacctgg acttgagcct
    14341 ggcggacgcg gcgctcaagc gggcggcggt gccggcgacg gtggattttt ccgcgccgaa
    14401 caagctgttc aacccgatgc tgtggcagaa aacgcccgag ggccggctgc tgaaggcgga
    14461 tgcgagcagt gtggggccga acgcggtggt ggtgacccgg cagacgcccc tttacctgaa
    14521 gctggcgctg gatgaggtga cgatgaccga ttcgggattg cgctgccgga ttgggatcga
    14581 gaatgaagcg gcgctcaacc cgcgggatcg caagaagcag cggtattgcc ggcagggcga
    14641 caagaacgag gtgtttgccg tgcgcgaggt gcgcccgatg ccgaacaacc cggccaacgc
    14701 gacggtggcg ctcgaattaa acgacggcgg cccgcgcgtg gaaatcagca aagaccaccc
    14761 gtttcagcgc gtcgaggggt acgtggtgga tttgaaatac ggcccggaga acaaggtttg
    14821 gaatgcgcgc cggatgggcg cgttgctgac tttcggcggc gaggaataca atatcgttga
    14881 tatcacggag gacgaaatag tattgttagc caagtcgaat ttgaagaaat ggactatcaa
    14941 acacagtgcc gttgccgcac cttgaaaccc gtttgtcctt agccaaacgc aactgagatt
    15001 atatgattaa agcagcctct ttgccgctcg cattgatttt actagccgcc gccgcgccgg
    15061 gggctttcgc gcagctcgcg ccgcaggacg ccgcggttaa cgaagcggtc tatcgccagg
    15121 cccaccgcat tacgctgcgc cagaagctgg cggaggcgca ggcggcgcag gagcgccggg
    15181 cgctggcttc cgccgccaag ctgtatgatg attcctggga gttggtgcag aagattggca
    15241 ccggggtgga ggcggagcgg gagcaggccg tcgccggcct ggccgcggtg cggctggaac
    15301 tggcgcgggc ggcccagcgg cgcggtgatt atcgggaggc gcggacgcag gtggatgatg
    15361 tgctgcgggt ggaccccacg catgcggcgg cggtggaatt caagcgcggc aacgagaaac
    15421 tgctcgccgg ccagcgcggc acgatcccca gtgaggaagt gcgcagccag gtcccggccg
    15481 tggccgagga aaagatcacc gccagcacgc tggtgcatga cgggaagctg ctgtatgaaa
    15541 tgggcaagct ggatgaatcc gaagccaagc tcaagcaggc gctgcggata gacccgcata
    15601 gtgaagccgc gctctactac ctgaacctcg tatcggaggc caagtatatc cgctccgcca
    15661 agcggcatga ggtgacgatg cgggaggaca tccgcgccgt ggaacaggcc tgggccaacc
    15721 cggtcagccg ggaaaagctg ctggttccca atccttacgc ccagaccaac ctgatccata
    15781 ccggccaggg gcggcaggcc atcatgatta aactcgaccg catccggctc gacagcgtga
    15841 aatatgacaa cctgccgctg ggagaggtga tcatcaacct gaacgacgag tgcaagaagc
    15901 gcgatccgga gaagcgcggc atcaatttcc tcattcacca ggccggggaa aatgccagcg
    15961 ccggccccca gccgatggtt ctggggccgg acggcaatcc tcttccggcg cccatgccgg
    16021 agcaggtgga catgagctcg attgcgatta agattaaccc ggccctgacg gatgtccggc
    16081 tggcggatgc gctggatgcc atcgtgcggg tggccgacca cccgataaaa tactccatcg
    16141 aggactacgc cgtggtgttc tcgttgaagc cgcgcgaggc gactcccgag cctttgtata
    16201 tccgcacgtt caaagtggac ccgaatacgt tctaccaggg cttgcaaggg gtggggtcct
    16261 atgtctttgg cgagtccatg aacgtgggtc agaacaccgg cggcggcggc tttggcggcg
    16321 cgaccggcgg cggcatgact ggcgggcaga accagaacca aatctcgggc gcgattatgg
    16381 cccgggtgcg catcgccggc ggcacggtta tggggggacg gcagggcggc ggcgggatgg
    16441 gcggaaccga gacggccggg ggcttgaact atctgacgcg gaccaacgac atgtcggagg
    16501 tgtcgcgcgc ggtcattaac ttcttttcca gcctgggggt ggacttggat ccttcgcgga
    16561 accccggcaa ggccctgttc tttaacgatc ggcagggggt gctcattgcg cgggccacgc
    16621 tgcaggatct ggatatgatt gaagcggcca tccaggtgct gaataccgtg ccgccccaga
    16681 tcaatatcaa gtcgaagatt atcgaaatct cgcagagtga caccaaggcc ctgggctttg
    16741 attggttttt ggggaatatg ctgatgaatg gcggcaagat cggcgggcaa cccggcaccg
    16801 ccccgtcctt taccggccaa ccgactcccg ccaatcccct gggagtgttc cccggcaatc
    16861 cgtatgcttc gcccccgacc accattgcgc ccacggccac ggatggtctc ctgacctccg
    16921 gcttgcgcaa cccctccacg acgctcttta cgcttaccgg catcctgacc gacccgcaat
    16981 tccggctggt catcaaggcg ctgcagcagc ggcagggagc cgagctgctg gcgcagcccg
    17041 aggtcaccac caccagcgga cggcaggcgc aaatgaaggc ggcggacgtt aagagtatta
    17101 ttaccagcta tagcttcagc caggcgcagt ccaccggggt gtccacgggc acggggacca
    17161 cggtcaacca ggcccagccc acggtggtct atccgattcc cgagcagatg gaactggggc
    17221 cggtgctgga cgtaatcccg tgcgtgctgt cggatggcta caccattaac ctgactttga
    17281 ttcccacgct gacggagttt gtgggctacg acaatcccaa tgaagtgggc gcttccacgg
    17341 cgatcaacca gtcgctggcg ggcggcttga tcatggttcc caccgtgctg ccgcatttca
    17401 cggtgcggca ggtggtttcc acggtcaacg tgtgggatgg ccagacggtg gtcctgggcg
    17461 ggctgctttc cgagacggtg acgacgatca aggaccaggt gccggtgctg ggcgatctgc
    17521 cgctggtggg ccggctcttc cgcagcgagt ccaagacttc cgggaagaag aacctgctga
    17581 tctttgtgac gccgacgctg attgacccgg ccggaaaccg gctgcatacg gatgacgaaa
    17641 tgccgtttgc gcagacgggc tttccggcgc aaccgccggt ggtggcggga gcgggaaaga
    17701 attgaccccc gtgacctgcc ggcctgttta atccgctgcg gctgtgagcg tcaaacccga
    17761 ggtcattgta atgcttgaac gtatgtcttt gccactgttt gcccggtcgc tggtcgcggg
    17821 cgcgctcctg gtttttgccg cgcccgtggc ggtggtgggc cagaccaacc tcttccagac
    17881 caacggggtg gagtacgcca tcgccggggc tttgaccggc gaccaggttc atcccggcct
    17941 gagccttaga agcaccggcg gctacctcgt ttgggaggac aacctcacgg acggcgacgg
    18001 cctggggatc agcgcgctgc agctcaacag cagcctgtct gggacctatt cccccttccg
    18061 cgtcaatgcg ctgggcgcgg gcgatcagga attcccccag gtttgccggc tgagcggcgg
    18121 cggggtggcg tttgcctggc agggcggacg gcgcggttcc cagaatatct acgcccggtt
    18181 cctgtcgtcc ggcggcacgt gggtgacgcc tcacagcgat gtgttggtca atacgtttac
    18241 gaacaacttc caaatcgcgc cggccatggc gccgctggcc aatggcaacg tggtgattgc
    18301 ctggggcagc tataatcagt ttagcgccaa cagcctgcag gatgtttatg cgcaggtact
    18361 tgcgccgagc ggccaaaaag tgggcggcga atggctggtc aaccagttca ccagctataa
    18421 ccagcgcacg ccgtccgtgg ccgcgttgag cgatggccgg tttgtcgtgg cctgggtgtc
    18481 cgagcagcag cgcttcgaga acagcgtgga cctttacgcc cggattttca atgcgaacgg
    18541 ctcgcccgcc agcgcggagt tggccattaa ttcctccacc aatgtctgcg ccaatcccca
    18601 tgtcgtggcc gcgcccaatg gcggatttat tgttgcctgg tccgagaaag acttgtccag
    18661 cctttggacc aacagttggg atgtttatgc ccgcgctttc cagcacaccg gggctgccgc
    18721 cggggcggcg gttcgcctga accagcaacg ccttggggat cagtatgcgc cgacgctgac
    18781 gttgaacggc gtcgagatgc tggcggtttg gaccagcctg ggccaggatg gctcgggcgc
    18841 gggcgtggtc ggccggttcc tgcgcagcga tttaacgctg tccggcgatg aatttctcgt
    18901 caacaccacg acggtcggct cgcaggtgca tccggtggcg gcggctgacg gggccgaccg
    18961 gtttctcgtg gtttggggca gtttcactgg tctggcctcg ggcttcgacc tttacgccca
    19021 acgctatgcc cgccaggttc agtcgctggc ggcgccggac ccgccgtttg tgaccgtttt
    19081 gagctcgaat gccctgagcg tgacctggcc gcccctggcg gggctcacag tggccgctta
    19141 cgaggtctat gccgacgggg cggcgacgcc gacggcggtg gttaccaacg cttggtggac
    19201 catgaccggc ttggcgcccg gttccaccca ttccttccgc ctggcttaca ttctgaccga
    19261 tggccgcgcc tcgcccattt ccggtgcgac gaccaacact acttacggca cgctcacctg
    19321 gggcggcatt ccgtatgagt ggatggttta ttattttggc tccgaccttt atagctggct
    19381 tttgctgtat ccgccgaatg ccgataccga cggggatggc gcttccaacc ttgaggagtt
    19441 tctggccggc acttctccca tcaatgcggc cagcgtcctg cgcgtcaagc tccaccccac
    19501 gccgcaggga ccgttcctgg actggaacac ccagccgggc ttgatctatc aggtgcaggc
    19561 atcgtccaat ctcaagcagt ggaccaacct cgggggcgtc cgcttcgccc cgggcacgac
    19621 ggattccatg tatgttggcg gctcggcggc tggttactac cgggtgttgc gcgtgcgcta
    19681 atggagcttc tgatatgtcg cgttttttta atataagaca gtgggcgggc tggatgttcg
    19741 ggctgagcct ggcgctcgtc gcgccgtcgg cggctgcctt ctcgatgctg ggcccgggcg
    19801 aatcgtggca ggacatcaca atggggtatc agaagttcca ttacataacc ttcccggcca
    19861 gcggttgggc gatccatggc aatgactttg cctggcatcc gcagaattgg ggggaggaat
    19921 tccgctggaa caataaggtg ttatattatg cctgtgacca gagcttcctc gactatttcg
    19981 gcgcggaagg ggtggcggcg gtggatgccg cggtggcggt tttcaacaac ctgcccaatg
    20041 cttccgatgc ggacctcaac cagtttccgc tcgaagccag ccgcatcaat tacacggccg
    20101 cggccctgca cctttacgat ttgaagtcca cgatcatgga gttgctcatc gagcggttgg
    20161 ggttgattga tccggagcgc tggacgtggt gtattcgcgc ccgggttctg ccgccctcgc
    20221 tggcctgccc tcagtttgat ttcgctgtgg tgcagcgcaa tttcgatccg gtgacggggg
    20281 aaccgacgcg ctatgtcaac ggcaccttgt ttacctacgg tatccagcaa atctgcactc
    20341 cgtatgattt cggcgacgcc gtcgagtttt tggtggatcc gcatgccgac gcttcggcca
    20401 ggtacagcgc gctggcgacg cccaagctta cgttgcccga cgagaccttt tatggctggt
    20461 ttcataccgg gctgacccgg gatgacatgg gcgggttgcg ccatctctac cgcaccaata
    20521 acatcaatct cgaaggaagc ggaaccaacg ccgtcactta cgtggtggat accagccgga
    20581 cgaacctgtt gttcatgtcg aacctcacgt tcctggcttc ccaggtgata accaacgacg
    20641 cggccacatt gcaggcgctt ttccccggcc tgagcattgc nnnnnnnnnn nnnnnnnnnn
    20701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn ctttccagtt taacaaggtc gggccaattt accttaatgg
    20821 cacctatccg cttttcgtgg atgagtttgg ggcgctgctt aatttcacct gggcttcttt
    20881 tgacgcgagc accaatgcgc cgattatcta tcccagcggc accagcctgg ccagcctcga
    20941 aaaccaggtg ttggttcagg tttccccgcc gtatttgccg gatgccgtca cgggcagcgc
    21001 gtacagcgtc caattgtaca ccagcgcggc cacgcccaac tggcaaactc ccattacctg
    21061 gtcgctggca gcgggttcac ccgccctgcc gccgggattg acgctttcgc cgggcgggca
    21121 catttccggt acacccgggc agatgggttt ctttaatttt atcgttgtgg ccagggacgc
    21181 cgcgggccgc accgttcaac agagttatgt tatcaatgtg gcagcgtctc catgaccacg
    21241 ggatcggccg gggcgccctg tcggagcgat tctttatgaa gcagaagtca attttgggtc
    21301 tggtggcatt ggcgctgacg tcggcggcct ggggccagac ctacgaaaat tggaccgtcg
    21361 tccagtgtcc cccggaaatt ccgccgaaca ttgttgcgtc caccttcatc aaccatgccg
    21421 agttccggat cgacttcacc aatttcaaca tggggtcgct gccggtcacg gcgccgccgt
    21481 atcgtgggtg gtcggaaacc gtcaacttca tcaacgattt cggcgctcac atgagctgca
    21541 ataccgggtt caatatcgaa acttttcccc tatccggctc cgggcagcgg gctgccagtc
    21601 tttacaacta cggaaccatt gactgcggca ccctgggcac ctccaatatt ttcagtatcg
    21661 gaggttttct ctttaacctg ctgggatcgg gggagggggt taaatgcaag gttaatgcca
    21721 ccaacattgt cagttcgggc acgatcaaca tgggtttcaa cagcctgctc accctcaagg
    21781 gcgatcggat tgacctcagt tacgggaatt tgataatgga aagcggtggc tttactggcc
    21841 tcggcggcgg cattttctac cagggcggat tttttgacgg ttattggggg aaaggcgacc
    21901 cggttactca gcggccgtta tattatccca atggcattga ccccgtttct ttgtacgaaa
    21961 cagtcccgcc gcgcacccaa tatcacatca tcaccaatcg caattacgcg gtcatcgaac
    22021 agcagttggg gggcggcact tttaatcctt acctcctgga cgttgttgat caatccggct
    22081 ccaatcgcac cgtccgcgcc gtatttctca gcaatacgaa tcccgccata accgccaacg
    22141 tctatttccc cgcgttcggc ggcgatccct atcaactcgg ccccgatatt atcgagtggt
    22201 cctggatttc tactaacttc gtgggcgtta ccaccaacta tctttacctc caggatactt
    22261 tcctgtccta taccaacttc cagcttctct tgaacggcta tgccggcatc ggctacaacc
    22321 gtcccaccta catccccgag aactaccact tctaccaggg caaccaactt tcgttgggcg
    22381 ttccggcggt ttccacggtg atcccgcccg gcacgttcct cactccccaa cgtaacgtga
    22441 ctaacgaatg gaccgcttat caggccattt tccagccggc ctcgactatt atgggcgagg
    22501 tggccggcca gaacgtcacc aacctgcccg ggcgcatcga gttgacggcc gacaaatacc
    22561 ttgtcctcac caacgcccac atcagcagcg tcaactatct gctgctgaaa gccaccaacc
    22621 acttcggagg cagtgccggc gcggagatta cggcccctca tgccgatctt tatctgcgca
    22681 gtaccaacgg cttcctgaac atcaccaacg tgctggcgcc ctacctgccc cgcccgattg
    22741 gcatctgcga cctgtttagc gcgcgctgga ccaacattat aaataacatc accaatcggt
    22801 accacgtgct cttcgtggac acccaactcg aaccttcttc gccgctgctg gtgcaaaccc
    22861 ttagcctgag cgccaccaat gccgcgacgc gcgatgaccg catcgttatt agcgatgtct
    22921 ttaacgtcac ctctaatctg ttgcttaaca cgcggcggct gactctggcg accaacgcgc
    22981 ccggtgcccc cgctccggcc ggcatcctga actatcttaa tccggcggtg ctgtggccgt
    23041 cggcgacacc gcggttgcaa tatctcacca atcatggcgg catccgggcg caaaacctca
    23101 tcgtgtttgg cgggtcacaa agctctccct acatcggacc ggacagttcc accaacccat
    23161 acgccgtttt tgtcaacacc ggcggcgtta ccaattttgg cagtttcatc tttgccacta
    23221 atttcttgaa tagtggcact ttcctggcca ccggcggcac ccttcagttg cggcaggcac
    23281 ggactgcggt gctcaccaat ggcgcgttcc tggcggcagg tgcggcgggc gccattcgca
    23341 ttgatgccga cagcctgttg gtgagcaacc atgtgttgca agccgggaat accctcacac
    23401 tttccgtcac gaatagcctg gatgacggca gtttggcgga cggggcggat tccgtcactc
    23461 atccgaacat ttggaatgtg ggttacggga tcaacctgct tacccgcccc gcccggggcg
    23521 gtctcctggc caccaccatt accaataccg ggcttcccgg ggccaaggtt cccaactcct
    23581 gggcaggatt ggaccgcggg ccggtggcgg cgggattttc caataatggc gcgctcgggc
    23641 ggctgatcct tgatggcctt accaacacca cccggttcga gttcacgccc gttacgggat
    23701 ccaatgcgct ttatgtggat tgtcttgaac tccggaatca catgactaaa ctggacggca
    23761 gcggcaacgt ggcaaatctc ctgttccagc cgggcatgaa gatttattac gcccaactga
    23821 ttatcaatgg cgtttcctgg gccgagaaac tgaacggcaa gaatggcggc ggtttgaact
    23881 gggttccttc ttatgcgggc acctacagct ccaccaacgt ggtttatccc gatggcacga
    23941 ccaaccggct gaaccaggcc ctggttcaaa gctgcaacct ggattccgat ggcgatggca
    24001 ttcttaactg ccaggatccc acgccggtat ttgtgccgtc ccaggtgggg ctgactgccg
    24061 cgcgggcgta tttgccccaa ccggcggtgg tgctctcctg gaacagtatt ccatattcga
    24121 ccaattgggt attctacaag ccgacgctcc agactgccca gtggcagctc ttgaccaatt
    24181 ttgtgctcgg ttccgccggc gggcgccagc aaatcgtcca accgtcggcc ctcaacagca
    24241 gccggttcta tcgtgtgcgc gtggacgccg cgatgccgta accggcgcag aggttgcggg
    24301 tgccgtcgga tcaagttagg accgggattt atgggagcgc aggaatgagt ttttgcgcgc
    24361 ccaaacgcaa cgaaatgtta tgcgaagatt acgaatgaat gccgcagtgt tcaaccggct
    24421 tgccaggggg ctgctcgggt tgctgaccgg gcttggtttg gcctcatcag tctttggtga
    24481 tgcacccgaa aacgatgact ttgccaatgc caccgtcatt tcgggaatct gggggacggt
    24541 aagtggcaac actgaagagg ccacggctga aacggatgaa cctgaacatg ccggtgccgc
    24601 cgccgccaat tcagtttggt acaagcttat tccaattcaa gatggcaccc tgacgattca
    24661 tacctttggc agtgccattg atacgcggtt ggcggtgtat agcattcctc ccaatacgaa
    24721 catagcggtt aacagctcgc tgtttcccgt tgctgccagc gatgacttcg atctccggca
    24781 ggatccgttt gaacgtccct tgcggggacc caatggcccc tacgcggaag ccggccccag
    24841 tgccgttcgt ttcccggtga agctgggcac agcatattat gtagtcgtgg atagcaatga
    24901 tggaggcggc gagattcagc taacctgggg gtatgatttt ggcgggcttt tctactttac
    24961 caaaccgcag gttgaagccg gcaaaaccga gggcagcatt cagttcgcgg tcggccgcac
    25021 ttttggctgc tcggggcggg tattggtgga tgttgtcacc acaaactata caggaaagaa
    25081 tatgccggcc accgaaggag tggactatat tccggggcag cagaccttga tcttcgatga
    25141 ttatgaaacc ctgcgaacgt tccgggtgcc gattatagat gctgaattcg ctggcgatcc
    25201 cggcgatccc gaagaccccg aagctccggt atcggcgccc tttaacccta attatcactt
    25261 taacgtgcag atcgtttccg tgcggtttca tgatctcgaa aacacaaata ttctagcccc
    25321 cccggcaatt cttaccggtc aggataccag cgtcggccgc atgctcgacg tcaatatccc
    25381 ccgtggcgcg ggcgatcccg ggccggatgt ggcagcgttc acgaataaca tcatcaactt
    25441 cgaagtgcgg cgccaggtga cgactgaata tgcgggggac ggcgatgggt atgtgcatgt
    25501 ctggttcacg cgctccggga cggccgatcc taatacaacc tgcagcatta aatggggcat
    25561 taatagtgcg tacagtctgg gtgatctccg caacaacctg tttgacttgt cgcccgagtc
    25621 cgattatgcc acgcccgatc cgcccagcac ccccgctcca ggggttgggc ctgccgattt
    25681 tggcccgccc agttcaggca cagtttcctg gggcagctat gatttcgttc ccaaggaaat
    25741 cttgatcccc atcaatgacg atagcctggt cgagtttaat gaggatttcc taatcgaact
    25801 tacgaatatc gacgcccaga attgccgcat gggcgaaatt aacaagtgta ccgttacgat
    25861 tctttttgac taccatgatc aacccgccgg cgctttagag gccggtttca acgcgcacac
    25921 agaacctccc aataacccgg ttccgggcac cgatgccgcc atttttgcca tggcggttca
    25981 gccagatcag cacatcctga tcggcggggc ttttacgact tataacgcgt tcctccgcaa
    26041 ccgaattgcg cgcgtcaacc ccaacggcac gctggatcgg acctttgatc cgggcaacgg
    26101 cgcggatggc ctggtcacga ccctggcatt gcagtccgat ggctacgttc tcataggggg
    26161 agattttctc gcggtcaatg gcttcaatcg ctaccatgtg gcgcgcctga cgccgtctgg
    26221 cgccgtagat acgactttca atcccggggc gggcgccgat ggcaccgttt ggtcgttggc
    26281 gatcagcacc aatgatagcg tgatcattgg gggtgagttt aacagcttca ataactaccc
    26341 gagctcgcgg gtggcccgtc ttgattcctc gggaaacctc gaccttgctt ttgatacgtc
    26401 cagccatggg atcaatggta ccgtatgggc cgttgcgccg ctaaccgatg gaaaggtgat
    26461 tattggcggt gattttaacc tggtaggcgg tcttttgcgc cccgggattg cccggcttaa
    26521 tgccgacggc tcggtggatg ctgattttga ccccggcctt ggtgccaacg atacggttta
    26581 tacgttatgg gtccagaatg atggcaacat ccttgtaggc ggagctttta cacagttcca
    26641 tacggcctcc catcatggcc tgacgcgact gttgccggat gggcaactgg atccggcttt
    26701 taatcccggt gctggcgcca atgatgttgt ttattctatt ttggtctcgc cgaccaactc
    26761 cgcgatatac gccgggggca tgtttaccac tttcaaccaa acgcgccggg ttgggctggt
    26821 ccgacttttc cctgatggca ccgtggacac cagttttctg gatacggctt ataaccagtt
    26881 cgcgggttta acgggccctt ggtttaatcc agaccgttat ccgcgtagct tcgtgtatgc
    26941 ccttggcctg gaagacgtgg aggtttatgg cattgcccgg caggatgttc ttattgcggg
    27001 cagcttcaac cgggttggcg ggggaggcac taaccgccga tcgtatgata ctcgagagaa
    27061 tttggccttg ctggtgggcg gggaaacggt cgggccgggc aacctgaccc tgactcaccc
    27121 gacatattca gtcaatgaga atggcagctt tctgtgggtt accctcacgc gtacgaatgg
    27181 caccttgggc agcgttggga cccggttttc gcccgatcca ttcccggttg gagccggggc
    27241 tgcggtctat ggcaaagact acacatacaa cccgttattc tatggccttt cggtttacgg
    27301 ctcgacttgg gggccgcccg ggggcccgga tacccgaatg ttgagcgatg gcatttggtg
    27361 gcgcaattac cccgacccgc gctcggtcct gccggaaatc caacagccgg gcccctttaa
    27421 caatgcccgg gtggacattc ttgataacac gatcaaagac ggcaaccgca cctttggtct
    27481 tagcctgacg attccctggc agctcgacac gtttttcctg ggaggcgcca atatgccatt
    27541 ggctactgcg ctggggccac agcccagcac gacttccctg ctgctcgata acgacaatcc
    27601 gcccggcacc ttttctttta tcactaccaa ctacacggtg agtgaaaccg ccgggcgagt
    27661 ctccattttg gttgttcgga ccaatggcac cggcggcacg gtgactttga agtacttgac
    27721 acaaaacctc gttggctccc cgcctccggg cgttggcttt gcccgcgcca gcaccaatag
    27781 cccaactccc aacggcaatt actatgccac gaatggcacc ctgactttct atccgggcat
    27841 taccagccgg acctttgatg tgaacatcag gcaggatggg cagatcaatc cggatttggc
    27901 cttgctgctc gtaattacca atatcgcagc tcaaaacccc gccagcggca cagctttggg
    27961 cggcattacc aatgcctggc tgaccattat tgacggcgat tttccacaag gccgcctaaa
    28021 tttcagctcg gttgccttta tgacgaacga aacggcgggt aacgccgtta ttacggtgac
    28081 ccgcaccggc ggcagccagg gaaccatgac ggtgctttgc tccaccacta acaccctggc
    28141 cagtgccacc aatacccccg ccgtgcccaa cgttaattat ttgccggtaa ccaacgtgct
    28201 gctgacctgg gccaacggcg acagtgcccc caagtcattt acggtcccgg tgctgcacga
    28261 caaccaaatt acctcgaacc tcacagtggg gctcaagctc tccacgccgg ttttgaatgg
    28321 catgaccaac tacaccgctt tgggcccggt cagcaatgcc gttttatcca ttctgaatgt
    28381 ggacagcctt ggatcgctat cttttagcag cccgcattac tcccagaacg agaacggggg
    28441 ctttgccatt attcccgtcg tgcgccaggg gggcagcgtt ggtactgtta ttgccacgtt
    28501 taacgcctcg accggcatca atgctactcc gggcatagat ttccttccaa ccaacgggac
    28561 gcttgtcttt ggcccgggag aactcagcaa ggtgtttacg gttcaactgc ttgataacgc
    28621 cattgtcgat gccccgaatc gcgctgtttt gctgcagttg accaatccgg tgccgccggg
    28681 aatcctgagt tctcccgcaa ccgcctcgct ctctctcgtt gatgatgaga cgtttaatct
    28741 gccgcccggc gggatggatc ccaattttca tccgctcttc aatggggccg ttcatgccgt
    28801 ggctttgcag cctaacggcg ctatcgttgc ggccggcagc ttcacgctgg ccaattatat
    28861 cacgcgccac cgcgttgccc gcctcaacgc cgatggcacg ctcgatttta ctttcgcttc
    28921 caagccgggc ggcgccgata acacagtccg cgccctggcg cttcaaacgg atggacgcat
    28981 gctgatcggg ggagatttcc tccgttacga tggcatcaac cgcagccgct ttgcgcgcct
    29041 gaatctggat ggcacgctcg attggcagtt cgatccgggc tccgccctca acgacagtgc
    29101 ttatgctatc gccgagacgt ttgccgatga cagtcgctcg gagcgcaaga tactggtggg
    29161 cggcaatttt acgctggcga atggcgcgcc ccggcgctac ctggcgcagt tcaacgacaa
    29221 cgggtcggta gatctggctt tcaaggtggc ggcgggcgcc ggcggtttgg acgggccgat
    29281 ttgggccatc gccgttcagc ctgatcgcaa aataatcatt ggcggggatt tccgctcggt
    29341 taacggcgag ccgcgcaacc atattgcccg gctcaatccc gatggttcgc tggatgccag
    29401 ctttagcaat tctggcattg gcgccaacag ttccgttcgc gcgctggcag ttcagcgcga
    29461 tgggcgcatc ctggttggcg gtctcttcac cagcgtcgag gacgctccgc aaaatcgtat
    29521 tgcccggctt aattccgacg gttcgctcga cgccagtttt aataccggag ttggagcaaa
    29581 tgacgcggtt tatgccattg ccctccaacc ggataaccgc attctgctcg gtggcgcctt
    29641 tacccgctgc ggcgatgttg ctcgttcccg ggtcacgcgc ctcaactcgg atggctccgt
    29701 ggatcccacc atcaactttg gcgccgggtg caacaatttt gtcgctgcga ttgccgttca
    29761 gcccgatgat gcaattgtcc tgggaggcgg ctttaccacc tacgacaacg cgagcgcagc
    29821 ttgcctggcg cgcatctatg ggcgctccat cagcggttcg ggcacctttg agtttacctc
    29881 cgcaatctac ggcgttgatg agaccgcccc gaacgccacc atcaccgtgc ggcggcgcgg
    29941 cggcaccagc gcgccgatta acgggaccaa tgtttatgtt acggtggcgg cgaccgacgg
    30001 tcctccgccc aacggagcgg taaacgatat taattataaa agcggcttgt ttacaaacac
    30061 gttccctccc ggagaaacct tcgcgacctt ccttgtcccc atcctgcggg atttccaggt
    30121 cacgcctgac ttggccgtga gcttgcagtt gaccagcata gacccgctgg gccttgccgg
    30181 gcttggcaac cagcccattg ccaccctttg ggtcagcaac gtggataatg ccgtgcgctt
    30241 ctcatctcct tcctacaccg tggccaagaa tgtccaggaa ggccgggcga cgataaccat
    30301 cgagcgcttc ggcagtgccg tgggcgccgc gcaggtggat ttcatgacca cgatggatgg
    30361 caccgcgccg ccttatggcc gataccttcc ggtggcgacc aacgtcgttt tcgctcccgg
    30421 ccagcaggtt caaaatgtct tcattcccat cattaacgat acgcaggttt tgggcaacca
    30481 gacggtgaca atggtgctga ccagtccaac caatgccctg ctgtccaccc cgtctgccgc
    30541 tacgctgact atcctggaaa ccagcacctc cccggggacc ctcgcgttta gttcggccag
    30601 ctatggggct ggcgaaggag acgcctttgc ctaccttgac gttcttcgca ctggcggttc
    30661 cgccgggtcg gtgactgttg attactacac ccgcgacgga accgcgctgg ccggggtaaa
    30721 atatgtggcg acgagcggtg cgctggtctt tgccgatggc gaaacccgca aaaccattgc
    30781 cgtgccgatc attaacaatg ccagcgtgga gttaaatcag acgtttttcg tcgtgcttac
    30841 caatgccaca ggcggggcgg ttttatccgg gcccacgacg gtgccggtga caattgtgga
    30901 tgatgatgta ggaatcagct tcggagcacc gattcacgcc gtttcggaaa ccgccggcac
    30961 ggtgtcactg tcggtcttgc ggcaaaacgg caccaacctg acaaccaccg ttcattatgc
    31021 cacgaccaac ctgaccgctt tggcgggcac gaattatgtg ggggtcagca acggcacgct
    31081 caccttcaat cccggcgaga ccgccaagtc ccttgccgtg cgaatcttgc gtgatcccag
    31141 ggtcacgggc gacctgcagt tcatggtgaa cctccgagat ccgtcggctc cggcccagct
    31201 tttcaactac agttcgaccg tggtcaacgt cctcgatgtg gataccggtt ttactttcgg
    31261 cactaccaac cggctggtta tcaccaacgc tgatttctct accgtgacta ccgccagcta
    31321 tggtgtgctc aagagcggca gcaacctgct ggtcaccgtg gttcgttcca acgcgaacac
    31381 cggcaccgtt tcggtcaact acgccaccgc caccaatgcc aacgatacgg ctattgccgg
    31441 cgtggattat ggcgccacca gcgggttgtt gacttttagc aataatgtct cggcgcgatc
    31501 gtttaccgtg ccgattttca atcaccgcca accggaaggg gaccggactt tcagcattaa
    31561 cctgttcaat cccagccccg gggctcagtt gattccgccc accgccgcca cggtcaccat
    31621 caccgacgac ctcgccgcca tcagcttttc aagctcggac tatcgcgtga acgagaacgg
    31681 cggctccgcc accattaccg ttctgcgcag taattacacg aacagcattg tttctgtgga
    31741 ttacgccacc accccgctga gcgccattcc taacgtcaat tataccaacg tcagcgggac
    31801 gctgaccttc aatcgcgggg aaacggtcaa aactttccgt gtgccggtta tgaatgacgg
    31861 gatactgcac ggcgatacta ccgtccaatt ggggttgagc aacccggttg gcaacgcggt
    31921 cttcattaat cccaacgcgg ccaccctgac cattctcgaa accgacggca gcttgatcgt
    31981 caatgccggg gcatccctga ttcgggaaag cggccctacg aacggcgtca ttgacccggg
    32041 agaaacggtg accctgctgt ttgccctgcg aaaccagctt ggcaccaata ccgccagcct
    32101 ggtcgccacc cttttggcta ccaacggggt ttccaatcct cgagccaact tttccaactc
    32161 tcccgcggtt cacaattacg gggtactggt gccgcgcggg ccctcgacct tcaggcctta
    32221 ttcgttcact gccgccggca cgaatggcca gcccatcagc gcgactttcc aactccagga
    32281 cgggaccacc gatttgggcc aggtcgtgtt caactttgtc ctgggccagt ctccgatccg
    32341 ctttggcaac tccgccgcca ttgtcatcaa cgattactcc agtgccacgc cgtatccctc
    32401 aaccattaac gtcagtggcc tggccggttt gctaacccgg gccaccgtta ccctgtccaa
    32461 ccttagccat tcctggccca gtgacattga tgccttgctc gtgtctccca ccggccaaaa
    32521 atcgttcctt atggccaagt gcggcggcag ttatccggtg agcaatgtca cgctgacttt
    32581 cgatgacgcc gctgccaaca gcctgaccaa cggcaagtcc gggccaatga tctcaggcac
    32641 caaccgcccc acctcctatg cgttggcaac accgcctttc ccgcccgccc tgacgccgcc
    32701 tcccccttac cataccaacc tgtcggtctt taatggcaag gatcccaatg gcacctggtc
    32761 gttatacgtg attgatgacg gcctcggcgg ggagggcgtc atttccaacg gctggtctct
    32821 cagtttgatt acggcgggcg tcgtcccggc cgccgccgat gttggcttgg ccatggcggc
    32881 ttctccgctt accaacatcg tcaacaacaa ctttaccctt accttggcgc ttactaatta
    32941 cggccccgcg cgcgccacca acattgtggt caccaatctc ctgcctgccg ggatccagtt
    33001 cgtgagcggt tctcaatcag cgggcagcct caccaaccat gccgggctgg ttgcctggtc
    33061 cgtgccctgg ctggaaacca acacctgggc cgtcagcgtg cttaccgtgc gggctaccaa
    33121 taccggcgtg ttcaccaata ccgcgaaggt gacgaccagc acctctgatt tgaatcccga
    33181 tgatgacatc gcctcggcca ttgtggcggt ggtggccccg accgccaacc tggttctttc
    33241 gctggccgat tcgcccagcc cggtaaggct taccaattat ttgacttata cgatgtctgt
    33301 ctccaacgcc gggccggcaa ccgctaccgg cgtggtcgta tccaacaccc tgcccgccgg
    33361 cgtttccttc atttccgctt cgccgggcgg agccagatac gctaccgccc tcacggcctc
    33421 cggccggttg gttgtcacat tcaccaatct ggggaatctg cctcccggag cgcggaccaa
    33481 tctttccgtt accgttcgaa ctgaagccgc gggcacgatt accgataccg ccggctgccg
    33541 ctcggagatc gttgacccct tcaaagccaa caacgcggct gcggtcaaaa ccatcgtcca
    33601 ggcggtgccg ctgccgctgg tctttgcgcc tcgttccggt gcgctggcga tttcgtggcc
    33661 cgcgtatgcc ggctatcgcc tggaaagcac tgccgatttg cggcctccgg tcatctggga
    33721 gccggtgacc gatgcgcaca tatccctggc ggggtcaacc atcaccgcca ttgttcccat
    33781 tggccccggg aaccgattct tccgcctgaa ttggtctgcc gcgccggcac cgctgcccct
    33841 tggcatttcg cacacgggcg ccaacgtaac catcgcctgg ccggcggacc tgtggaatta
    33901 caacctggaa agcgcggaca ccctgagccc gccgctggtg tggactcgcg ttaccagccc
    33961 ggcgtcggtg ctggttaacg gattaaatac cgtcaccctc ccgattggct ccagcagcca
    34021 ggtcttccgc cttcgcgcgc agcaaccgta agccgttttt gccttatgcg ccttattccc
    34081 cacatgaagc cctccctcga cctggcccgc cgctttaggg gcctcccgcg cctcgcttgg
    34141 gccgcgcttt ttctcgcctt cgtccagttc gcggcgggtg aggaatacaa caaaaccaat
    34201 ctactggtcc ggtttgatgc cgaagtccag tcggcgcagc gcacgaatat cctgaacgcc
    34261 ctgggcggag ccacgatcag ccagaaatat actttggttc ccgacttgta cttggtcacc
    34321 ctgccggctg gccagcaagt ggaccaggtg ctgtcggcct acaacgccac tcccggcatc
    34381 cgttacgccg aacccaacta tgtggtcaga gtcgcggcca ccactcccaa tgatccgcgc
    34441 ttcagcgaac tctggggcct gcataatacc gggcaaacgg gaggcactcc gggagccgat
    34501 attcgtgccc ccgaagcctg ggattttaat accggcagcc gcgaaatcat tgtcgccgtc
    34561 attgacaccg gcgtggatta tacccacccc gacttgacca ataacatctg gactaacccg
    34621 ggcgaaatcc ccggcgacgg cattgacaac gacggcaatg gttatgtgga tgatgtnnnn
    34681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttt tcctgctatg gcttgacttc
    34801 cgtggatttg gcggcgcccg gcagcgacat cctcagttgc cagcccggcg gcggctatca
    34861 gtatatgagc ggcacctcga tggccactcc gcacgtttcc ggcgcttgcg ccctcttgct
    34921 ggccgccaac cccaacctca ccgtcgccca ggtcaagcaa gccttgctct cgacggttga
    34981 tcccgtagtg ccggggcttt gcgtttcggg cggccgcctt aacctgagcc gggcgcttgc
    35041 cagcctcgag ccgcaagggc cggcgctgga gtatttcacc aattacgtca cgggcggcaa
    35101 tgggaacggc atcgttgagg cgaacgagtg caataccctc gacctcgttt tgtccaaccc
    35161 cagcatgacg aatatcacgg gcattaccgt caccctctcg acgactaatg ccggggtcgc
    35221 catcccccag cccgcctcgg cttatcccaa tattcccccg ggagcttcgg cgaccaacct
    35281 gacgccattc agaatcagca ccgcgccgga gttcgtttgc ggcaccccca tcgagttttc
    35341 gctggtcgtg cgctccgacg ttggcgtcag cgtcctgccc ttcaccctcg ccaccggcgc
    35401 cccggggatc cccatccggt tcgacaacaa ttcaatggtg caaattcccg cgcccggatc
    35461 caccaactcc gtcatcgtcg tctctaatat cgccctggct ctcaacaagg tcaccgtttc
    35521 cctctttatc gaagagccgt atgattattt cctgaaactg gagctgatcg ccccggatgg
    35581 cactacgaac ctcctcacag ccaacaacgg ttacctgggt gaaaattatg gcctgacctg
    35641 cagccccgag gcgttgcgca ccaccttcga tgacgacgcg ctaacgccca tcggctccgg
    35701 cgtgccgcct tttctgggcg cctaccgccc cgccaaggcg ctcgcgattt tctccggcaa
    35761 atccggcacc aacgtcaatg gcacctggca acttcgcgcc accgactccg gcgggttcag
    35821 tcatgccgtg attcactgct ggtccctgtt cctcacgccc acgatttgcg ctgacggcgg
    35881 cggccaatgc cccggcgccg atatggccat tggcatcgtc ggtcaacccg ccgtagcgat
    35941 tgccgagaac aacctcactt attccatctc ggtcaccaat cttggcccca gcagcgccac
    36001 caacgtgacg gtgacgcacc tgctgcctcc cggcgcggcg ttcgtttccg ccaccgcgtc
    36061 ccagggcgcc tggtcgcaac agggcggcgt gctgacgttt acgctgggga ccatgccgac
    36121 tcgcacccag gccaaccttt ccgccgtcgt gcagccagcc gatttgggca cgaattacac
    36181 ctgctactcg accgcgaccg ttacttccgg ccagccggac tttgtccccg ccaataactc
    36241 ggcgacgacc cgaaaccagg taacgccacc cgcggcggac ctggcggtgg gaattgcggc
    36301 gacgcccaac cccgttttgg tcggcggcac cctgacttat accgtctcgc tcagcaacaa
    36361 cggtcccagc ccggccccgc ccattaccgt gaccaatgtt ttgccgccga gcaccgcgct
    36421 gcaatcggtg gccgtctcgc aaggcgccac gaccattctg ggcaacgtgg ttctttggag
    36481 tctaaccaac aaactcgcca tgggagcctc ggccaccgcc acgatcaaag tcactcccac
    36541 ggccgagggg gtaatcaaag ccaccgctac ggccgacgcc ggctcgtttg atcccgtcgc
    36601 ggccaataat tccgcgtcgg tctctaccgc ggttggcccg gcggcggatc tcgcgctcag
    36661 cctggtcggg cttccgaacc cggtcgttgc cggcagcaat gtgacttaca agatttcagt
    36721 ttccaacctg ggtcccagca ccgctaccga tatcaccgtt agcgatctgt tgccgcccga
    36781 aatgattgcc ctttcggcca ccgcaaccca gggatccggg gcggtttcca atcacaccct
    36841 tttgtggaac ctgggcacct tgtccgccgg ggcggaagcc tccattacca ttgtggcggc
    36901 caccacgacc aacggagtgt ttaccagtac ggccagtgtt ttggccgcgc aagcggaccc
    36961 caatctcacc aacaacaccg ccagcgcggt cataacggtg acggcgccgt ttattgacgt
    37021 tgtttccgcg ggcgccacct tgacctttga gagcgggccg gttaatggcg cgcttgattc
    37081 cggcgaaacc gttaccatga ctttgcgctt gcgcaacgcc ggcaatagca gcacccgaaa
    37141 cctggtcgcc accttgctgg ccaccaatgg catcgtcccg gttcccccca accagcccca
    37201 ggcctacggc atcctgttcc ccagcggcct tgcggtcgga cgccccttta gttttgcggc
    37261 caacggtccg gcgggcggca ccgtcagcgc cacgctcctg atccaggatg ggaccaatac
    37321 ccatccgccg gtcagctttg ccttcaccct gccgtccacg caggtcgttg ccaataccaa
    37381 ctccattatc atcccggatc cggcggcgcc gaatcccccg tacgagcagc aatccggccc
    37441 ggcgaagcca tatccgtcca tcatcaacgt ctccaacttt accggcacgc tgggccgggt
    37501 ggccgtcacg ctctcgaatc tgaaccacag ttatccggcg gatatgagcg tgctggtcgt
    37561 cgccccgggc ggcggccagg cgctgctcat gtcgggcgct ggcgatcagc ccaccgtcgg
    37621 cggcgtcaac ctgacctttg acagttccgc cccgaacgcc ctgccggaat acggccagct
    37681 cgtatccggc agctatcagc ccgccgctta cggcgacgcg ccctccttcc cgaccaatgc
    37741 cccgcccggc ccttacgcga cggccctctc tgccctgaat agcgtgaacc ccaatggcgc
    37801 gtggtcgctc tatgtttacg accagggctc cggcgatgac ggcgtcatcg ccaacggctg
    37861 gagcctcacc cttacgcgca tcaacccggt taatcacctg gccgacctgg ccctgacggc
    37921 gacggtttcg cccagccccg ccctggtcgg gggcgcgctg acgtatattt ataccatcac
    37981 caatggcggg ccaaacacgg cccagaccgt cgcgttcacc aatgtcttgc ccgcgggatt
    38041 cacgctggtt tccgccggcg cgtcgcaggg aaccgtgttc gccaatcccg ccagcgtcac
    38101 cgtgaacctc ggcaacctgg gagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38221 nnnncttgac caacctcgtc ctcatcacga atctcggccc cgcgaccgcc gtcagcgcca
    38281 cgctgaccca aacgctgccc gccggcgctg gctttatcgc ctccgcttcg agctcaaccg
    38341 ccggggtcat caccaatgcc ggcggcacaa tcacctgcct gctgggcaac ctggcttcca
    38401 atgccacggc ggccgtttcc atcgttctga ccagttcaac cgccggcttc ctgaccaata
    38461 ccctcaccct gggcagcggc tcctatgatc cagcccccgc caacaacacc tccacgcgcg
    38521 tggccaccgt cgtggatccg gccccgcaaa tcattaacgc gggcgctgtg ctgacgtatg
    38581 aaagcgggcc cgtcaatggc gcgattgatc ccaacgagaa tatcaccctg tccctggcgc
    38641 tcgccaatat cggcacccgg gtgactggca acttgaaggc cacgctgctg aattccggtg
    38701 gcgtcaccgc ggccagcggg cctcagtatt accccgcttt ggccccgggc ggcggcgccc
    38761 tggcgcgcac tttcagcttc aaggccgcgc cggccctggg cagcgggttg gtcgccaccc
    38821 tgcagctccg ggatgagcgc ccggggatca ccaacatgct ccccaatgtc gtgttcacct
    38881 ttgtggcgcc cgccacaatg aacttcacca atgccgcggc aattaccatc ccggatcgcg
    38941 gcggcggcgc cccgtatcct tccgtcatca acgtcgaggg attgacgggc cgggtcagca
    39001 aggcgaccgt caccctcaag ggcttcgcgc accagttccc gcacgacgtc agcgtcctgc
    39061 tggtcagccc gtccggcacc aacgtgctcc tgatggcgca taccggcggc ggctatccga
    39121 ccaataacct caccctgacc tttgacgaca gcgccccggg ctacctgccg aattgggatc
    39181 ggctggccag cggggtttac cgccccacct tctacgaggg cgccgtgacc ctgccgggca
    39241 ccatttcatc ccaatactac cagcataccc tctccgccct gaactggagc catcccaacg
    39301 gcccctggtc gctttatgtc ttcgacgacg caatcggcga cgccggcgtc atcgccgggg
    39361 gctggagcct gagcctgaag acagtcgtga ccgtgggcaa cgtggtgaac ctggcggccg
    39421 ggctgctcgc gcccgcaacg ctcgacctcg gcgccgcttt gaccaacacg atccgcatta
    39481 ccaactcggg ccccgacctg gcgaccggcg tgatgctcac aaatctcctc ccggcgaatg
    39541 ccgacctcct cgaggccgcg ccgccgccca gcttcaccaa cggcaatggc ggggtggtct
    39601 ggaacctggg cgaccttgaa ccgggcgccg tggcggaatt ccacaccgtc gttctgccgg
    39661 cgcaggcggg aatggcgacc catatcgtta cccttggcgc caacgaggaa gacctcaatc
    39721 cggccgataa cacagcgcaa accacgacgg ccgtttacta tgcggggccg gccatcctga
    39781 gcggcttcta cgcgggcggc cacttccacc tgaccgtcac ggcccagcgg ggcttccagt
    39841 acgtggtgca gggatcaacg aatcttaccc attgggtcgc gttgcacacc aacgccaacc
    39901 cggccggcac cttcaccttt accgatacca cgactccggc gctgcgctcc cgcttctacc
    39961 ggacgctgcg ccgttgagcg cgcgaacgct gttcgggccc cggccatgaa agtcctcggg
    40021 ctcaccggcg ggattggcat gggcaagtcc acggccgccc aatggctgcg ggatcacgga
    40081 atcccggtgg tggacaccga cgatttggcg cgccaggtgg tcgagccggg ccagccggcg
    40141 ctggcggaag tccgcaaagc ctttggggcg gacatcgtgg gccccgacgg acgcctgcgc
    40201 cgggacgccc tggcgcaacg ggtgtttgcc gatgcggccg atcgcgcccg gctggaaagc
    40261 ctcctgcatc cccccattcg cgcgctgtgg cacgcccgga tggaaacctg gcgcgcggcc
    40321 gggcatccct tggccgtggt ggtgatcccc ctgctcttcg aaacccgcgc cgagccggaa
    40381 ttggatgcca cgctctgcgt ggcctgcacg gcggcgaccc aacacgcgcg tctgcgggcg
    40441 cggggctggt cgcccgaaca gatcgcgcaa cgcgtgcggg cgcaatggcc cattgagcag
    40501 aagatcagcc gcgccgatta cctcgtttgg acggaggcgg ggctggaggt gctcgccgcg
    40561 cagctcgaac gaatcctgca agggctgaat tgaccctccc c
//
LOCUS       DAOPQG010000019        39672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1336_length_39672_cov_15.120176, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000019 DAOPQG010000000
VERSION     DAOPQG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 39672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39672
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1336_length_39672_cov_15.120176"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            478..1407
                     /locus_tag="PKI07_03550"
     CDS             478..1407
                     /locus_tag="PKI07_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50158.1"
                     /translation="MIPAGRSVWRRLTPREIRMLCYLGVLLGVAIWRFAPRPWHPAHT
                     VEVPHYVIQSSATELQTAEAGQALEMLYSAYTSRFEGWAQFQPAHPKLKVRLFRDRAE
                     FRRINPGLGWAEAFYRKPYCQAYYEAAEINPCHALLHEAVHQLNEEVTGVALAQWLEE
                     GLATYFSTSRMISNRLAVGRIDVNTYPVWWLDELAVSPHLAENLRNGSVIPLLAIITN
                     RGGPSLNRRVNLYYLHWWTLAHFIFEDSQYGQRAGFLVRQGGGLESFEATIGPVEKVQ
                     VEWHDYVRRLKAAWTAGDVRFFRDGILPEPAKS"
     gene            1595..1996
                     /locus_tag="PKI07_03555"
     CDS             1595..1996
                     /locus_tag="PKI07_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOC50159.1"
                     /translation="MSTDVTSAAGLGPRRGAGRRVASRRLEREWRTIQAMVRLYCRAH
                     HAGGRELCAECQGLLAYAAVRLERCRFGAEKPTCARCPVHCYQPARREQVRAVMRYAG
                     PRMVWRHPVLSWWHWVDGLKRPAGGGRRGRG"
     gene            complement(2069..3031)
                     /locus_tag="PKI07_03560"
     CDS             complement(2069..3031)
                     /locus_tag="PKI07_03560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC50160.1"
                     /translation="MNPGQGKLLVIRGGAIGDFVLTLPAIASLRRQFPAAHLEILGYP
                     HIAQLALAGGLVNRVQPIEAANLAGFFARGGRLAPELISYFAGFDVIVSYLYDPDGIF
                     RTNVALCTGADFIPGPHRAMDGGGLHAAKVYLQPLERLAIFDADPVPRLRLPPPPAPP
                     GPLALHPGSGSERKNWPEARWAELLHYLATATDFELLLVGGEAEGDRLKRLAAPLPPA
                     RVRVAQSRPLAELARHLAGCRAFIGHDSGISHLAAAVGLPGVLLWGETAVEIWRPPSE
                     KMIVLRHPAGLAQLPVETVLTEVRQMIGAFPVETPPRLNPGNRE"
     gene            complement(3063..3626)
                     /locus_tag="PKI07_03565"
     CDS             complement(3063..3626)
                     /locus_tag="PKI07_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50161.1"
                     /translation="MTLKLSTLSLLLGLGVGLPLLYGLAKPAAFAAAVRKFPRSLPWG
                     LALMALGTAWFLWNLSQESIADFARFKSHLFVGFAAIGLGACIFVQDFLAVRGLAIVL
                     LLLAKLMVDSGRPLLSQTHWVLINQTWAYGLVLAGIWLTISPWRLRDFLAWGTATPQR
                     TQVACSLGLAFGLFVAALGLTVFRTVS"
     gene            complement(3628..5052)
                     /gene="miaB"
                     /locus_tag="PKI07_03570"
     CDS             complement(3628..5052)
                     /gene="miaB"
                     /locus_tag="PKI07_03570"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOC50162.1"
                     /translation="MPSVYIKTYGCQMNERDSEAVAAQLVAQGYSLAPSEASADVILL
                     NTCSVRDLAEQKALRKMENLVAETRRRRSRVVLGFLGCVAQNRGSWLIHRLPDVDLIV
                     GTQKLHRTAEHLDRLFTGQRGKVVDVAAEAGSEAAIREHLLDGNARKSVTAYVSIMQG
                     CNQFCTFCIVPHTRGQERSRTIRDIVAECRELAAQGVKEITLLGQIVTSYGRGLIPRR
                     DGRTPFVQLLEAVNEINDLERIRFSSPHPKGYTDDLVEAYGRLGKLVENAHIPVQSGS
                     NSVLKRMHRGYTRERFLEIIGKLRRVQPTIGISTDFIAGFPGETEADFEETLRLVREA
                     ELDQAFVFKYSPRRDTPAAQMPGQISQAVKEERNQRLLAAVNESGARQHQKLVGRAVQ
                     VLVEGPSKKNPSRLTGRTRCNKIVVFEGPPHCRGQLLDVRISHAGTFTLYGQTGPPPA
                     GTVAKRRHSQLAPRGLIAHPPHGC"
     gene            complement(5119..6465)
                     /locus_tag="PKI07_03575"
     CDS             complement(5119..6465)
                     /locus_tag="PKI07_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC50163.1"
                     /translation="MSFPAPAAKTPRAGWPTALFAALWGAFLGLALLKFGNPPITEQW
                     VVPPEGIYEFLFGYPWPIAWAYGMLAGVAVLGAAVAHWRSPAPGWLIALPLIWLGWQG
                     LAATASADPSLSTATLKHFGACVLCFYLGYFSLRRVKQAWLFWIGLLGALLLVLAVGW
                     EQHWGGLRQSRQYFFLYVYPHLETVSPDYLKRITSDRIFGTLFYPNTLAGVLLLLLPV
                     LLTALGQLRAFLTPAARAFLISLAGMAGLACLYWSGSKGGWLLMLLLGLLALLRVPFS
                     RRLKTLLVAAILLAGLAGFFWKYASFFRKGATSVVARFDYWRAAAQTVRAHPWLGTGP
                     GTFAIAYQQVKRPESEMARLAHNDYLQQASDSGIAGLLAYTGFILGALVWGFPRARPK
                     RPAGPEYALAFAAWLGVLGWSLQSLYEFGLYIPALAWPAFTLLGWLLNRRESETPR"
     gene            complement(6467..7537)
                     /locus_tag="PKI07_03580"
     CDS             complement(6467..7537)
                     /locus_tag="PKI07_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOC50164.1"
                     /translation="MAFKDFPAQAQGVELLQRSLERQRLAHAYLFAGPHLDSLEALAR
                     TLAQTLNCQHPVRRNGAAVDCCDRCSGCRKARDGQHADVFWVRPESKLRQIKIGQIVH
                     RDNSPPRVLLDVVNLKPTESQYKVGIIVAADRMTEEAANAFLKTLEEPPPHSVLILLT
                     TEPERLLETIRSRCLRLNFAGEGPPAWAAAQLEWLTAFSEMAAASQKSLLGRYRLMDV
                     LLRKLKTVRDDIEKDLNARSPLEKYEEVDAALRDQWTLELNAAVEAEYRRQRGEFLLA
                     LQWWLRDVWLLSLGESAGSQASLRSLLAFPQLAGTHPVARRLSPKAARENLCILEQLQ
                     RYLQTNVQEALALEVGLLKLHL"
     gene            7629..7832
                     /locus_tag="PKI07_03585"
     CDS             7629..7832
                     /locus_tag="PKI07_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOC50165.1"
                     /translation="MECKKSRNAQFCNCTYPGCEKHGICCECLQYHLGMRQLPGCCFP
                     KEVERTYDRSFERFAALVNAGQV"
     gene            8121..9377
                     /locus_tag="PKI07_03590"
     CDS             8121..9377
                     /locus_tag="PKI07_03590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC50166.1"
                     /translation="MKIKGLRWYIAGLLCLASALNYLDRQTLSVLIGTIKEELSLTNA
                     SYGAINAWFLASYGVMYAVSGRIIDYIGTRRGFVLFVSGWSLANILHVVAATVGQFSF
                     FRFLLGVFEPGSFTGGVKAVGEWFPMRDRALAIGIFNAGTAVGSVAAAPIVSFIAVTW
                     GWRSAFLVTGALGFVWLVPWLLFFRQPKTHPQLSESELRLILEGQPEETAPPPPPRLR
                     QLLQMRETWGCVLVRALTDPISYFLLFWIPLYFQKQHGFCLQRIGLFIWIPYAAAALG
                     NLCGGIIPRTLIGRGWSLDKARKGTMIFLTFTLLVCLFAVTRTANPMAALLLVAGMTF
                     CHGGWGNITLPAEVFPKSAIGTVTGLGGALGSWVGALAMLSTGHVVDAYGFTPIFITC
                     ALFYPLALLLVYVLIGKLGVVRALPQ"
     gene            complement(9408..10148)
                     /locus_tag="PKI07_03595"
     CDS             complement(9408..10148)
                     /locus_tag="PKI07_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOC50167.1"
                     /translation="MPESAPKAKLVQMVLYGARVIPVRGTYDDAFQLSLEYTAHKGGL
                     NRNTAYHPLTLEGKKTVGLEIYEQNRFKVPDAILVPVGDGVIISGVHKAFLDLKAAGL
                     TARLPRLICVQAERSAAIHHYIRTGKYQNARRPATIADSISVSVPANAHLARRAVQES
                     RGFSLIVTDREIVRGQRTLAEKTGVFAEPAATATVAALRRLQGTDLLGRREQIVLLIT
                     GHGLKDVDAAARFLRFPAAAGPRPDAPL"
     gene            complement(11185..12204)
                     /locus_tag="PKI07_03600"
     CDS             complement(11185..12204)
                     /locus_tag="PKI07_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC50168.1"
                     /translation="MGPASLGAASVLAQRGYQVTVYEQWKRLGSMMRLIPNDRLDPQV
                     ANRDIEFIKALGAVDFRPGQGITDPAALLKDYKAVIVSAGLEEPMRLGIPGEEYALSW
                     QEYLERQKQIAVAGRKVAVLGGGAVAVDCAMTARKHGALSVELVYRRRQQDMPLTAYE
                     REILLEQGIEITSCCKPLEILHRGQKATGLRLARMMLPQGKAPRPQNFVVNRRESPIF
                     REFDVVIIATRSRSSLPIRKVRGAFYAGEMVLGSATVVESVATGKNAALEAEAYLLGK
                     KPPRLKNRAKSHVVLSGLELYPVPLETEFFGRKIRSPFCSRRRRIRTVMPKCARHTRR
                     VGPAG"
     gene            complement(12319..12615)
                     /locus_tag="PKI07_03605"
     CDS             complement(12319..12615)
                     /locus_tag="PKI07_03605"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50169.1"
                     /translation="MRALAVGKMAMKNNDGSLTAAQLQAELNRCEYCEEKPCQVACPA
                     DCSLADFIIAARMGAPSDYRRAGALIMGANPLGWVCGVVCPGLLLHEGMCAPHV"
     gene            complement(12740..13366)
                     /locus_tag="PKI07_03610"
     CDS             complement(12740..13366)
                     /locus_tag="PKI07_03610"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOC50170.1"
                     /translation="MPVKVKICGITNEADGLAAAEAGADALGFVFYEPSPRHISVDAA
                     ASVIRKLPASVIKVGVFVNAPVGLVLHAARECGLNLLQFHGDESPEYCLGFGLMSMKA
                     FRVRDRASLEAVRAYRTDAWLFDAYTPGLPGGTGETFNWNLARQARDWGRPIFLAGGL
                     SPENVAEAIQCARPYGVDVSSGVEAAPGKKDHARVKTFIEATKAVEDG"
     gene            13562..13637
                     /locus_tag="PKI07_03615"
     tRNA            13562..13637
                     /locus_tag="PKI07_03615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13596..13598,aa:Arg,seq:cct)
     gene            complement(13666..14199)
                     /locus_tag="PKI07_03620"
     CDS             complement(13666..14199)
                     /locus_tag="PKI07_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOC50171.1"
                     /translation="MKNIVYFDLETQKSADEVGGWENISRMGMSVGVTFSTARGAYEI
                     YCEKRVNELIMELQRADQVVGFNILRFDYEVLHGYTAFDLRQLPTLDMMLKLQETLRH
                     RLSLEAIAAATFGLKKTAEGLQAIQWYREGKLLEIAEYCCYDVKLTRLVHEYGAQQRQ
                     LHYHNRFGRKLTVPVNW"
     gene            14363..15757
                     /locus_tag="PKI07_03625"
     CDS             14363..15757
                     /locus_tag="PKI07_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50172.1"
                     /translation="MKETAHPLTSRREFIKTTGRFTAASALAGVALPHVHAAGSDLIQ
                     LALVGCGGRGTGAANNALSTQAGPLKLVAMADVFEKRLADSYDNVTNSKRDLVDVPPS
                     RRFTEFTGYQQAMDCLKPGDIVILTTPPAFRWVHFAYAIKKGLNVFMEKPVTVDGPTS
                     RRMLKLAEDAAARNLKVGVGLMSRHCRALQELRQRIQNGEIGDVILMRGYRMHGPVGH
                     AFQTKWPGDPSELLWQVRHFHAFLWASGGCFSDFYIHHIDHLCWLKNAWPIKAQALGG
                     RHYKQNPEGVPYVDQNFDSYSVEYTFADGSKLYMDGRCMNGCNDIYSSYVHGSKGMAI
                     AAKAGDFQGPSSIFKGQNAQRSNLLWTSKPPPGQTDDPYQNEWDDLMAAIRNNQPYNE
                     AKYGVEASLVTSMGRMAAHTGQEISYEDMLNCEHEFAPDVDKFTMNSPAPLPAGPDGK
                     YPMPMPGITKQREY"
     gene            16091..17377
                     /locus_tag="PKI07_03630"
     CDS             16091..17377
                     /locus_tag="PKI07_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOC50173.1"
                     /translation="MKDIVPAPPPVVRNDVAAVQTLFQQNVVPSYGRFDLVLSHGAGC
                     SLYDITGRRYLDLGGGIAVNSLGHAHPAITEALAEQSRKIIYCSNFYYHEPQGRLAQA
                     LVSLIGPGKCFFCNSGVEANEGLFKLARKFGHDEGRFEILTTLNSFHGRTLAAIAATG
                     QEKVKKGFAPAVPGFRHVPFNDLKAMREAISPATAAIMIEGIQGEGGVLPATPDYLLG
                     LRRLCDEKKLLLLMDSVQCGHFRSGRFQSYQRILEAVPGGETFLPDGISMAKSLGGGF
                     PIGAFWVRAPYADLLSAGSHGTTYGGSPLACAVALKILEVIARENLAANARQVGAQFK
                     SRLEELARKYPAIIQKVRGLGLMLGMELAPAMPQLPGDPAKTPAVRFINLLHAAGLLA
                     IPAGTHVIRFLPPLNLRPAEAEEGLKMLESVLANLV"
     gene            17421..18317
                     /gene="argF"
                     /locus_tag="PKI07_03635"
     CDS             17421..18317
                     /gene="argF"
                     /locus_tag="PKI07_03635"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOC50174.1"
                     /translation="MRHLLSIEKLARAEMDAILAGVAPLKKERGRHTRFPLAGQTWAM
                     IFSKSSTRTRVSFEVGIRELGGQVIFLPAHEIQLGRGEPVKDMARVMGRMVHGAIFRT
                     FAQQDVEEFAHYAQIPTINALTDQEHPCQILTDIFTFQEKRGPIQGKVISFIGDGASN
                     VATSLVFAAGKAGFELRLAAPRGFQPDAGLVKRAGGKIVVTEDVKAAAAGADLLYTDV
                     WVSMGREQESAERLQQLSGYQVNAGIVRLARPDALVMHCLPAYRGKEIDEETFEAHAQ
                     TIFDQAENRLHVQKAILNWMTG"
     gene            19879..21099
                     /gene="aroB"
                     /locus_tag="PKI07_03640"
     CDS             19879..21099
                     /gene="aroB"
                     /locus_tag="PKI07_03640"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOC50175.1"
                     /translation="MRIVRVSLGQRSYNIQIGTGLLARVGNLCRRLELGRRCAIISDA
                     NVAPRYAHATQKALDRAGFATELITIPAGETAKSLGTVAACYNQLAGHRLERSSFVVA
                     LGGGVVGDLAGFVAATYLRGIPFVQIPTTLLAQVDSSVGGKVGLNLKAGKNLVGAFHQ
                     PRLVLCDLCTLASLPPREYLAGLAEVIKYGIIYDAALFRVLERDLPRLLRREPACLAG
                     VIARCCEIKAAVVQQDETESGLRAILNFGHTIGHALEAISRYGQYLHGEAIAIGQVAA
                     AQISVCLRGLPVSEALRIELLFQRAGLPTRVKLTAARQRKLFAAMKLDKKVSRGEVRF
                     VLTPRIGVAEFGHPVPAGILQRVLNPEPGAAPGLEQGQECPPRPSGQAEGGSACPRPG
                     VAAAHSPHPTPSHV"
     gene            21101..21706
                     /locus_tag="PKI07_03645"
     CDS             21101..21706
                     /locus_tag="PKI07_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50176.1"
                     /translation="MSAVSETLVREYFELHEFLVRQHRKHVGQTRRNEDDDIDFFVLN
                     PHPKPAMGELPFVLGSPELSFIERAIVVVKGWHTDTFSPARLEASPGIFRFVEPRVFR
                     QAARAFGKEDTPLKVLVVPALPQTAAARDESIALLRSKGIDAVIPFRTMLADLISKTA
                     PNFNYQKSDVLQLIRILKNYDFFKEPQMELFKPRPGKIRKG"
     gene            22050..23405
                     /locus_tag="PKI07_03650"
     CDS             22050..23405
                     /locus_tag="PKI07_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOC50177.1"
                     /translation="MAQQQTLKGTASFAGIGLHSGNRVSMTFLPAPPNTGIRFRRVDL
                     EGKPEIEAIAENVVDNNRSTTLARGHTRVHTVEHVLATFAGYGIDNAIVELDANEPPI
                     GDGSAREYCKLIQEAGIVPQDERRECYKVTEPIQLEMGETVMTLFPDEAFRISCTSAD
                     KQGRFTQFYSVELTPTTWERDLAHARTFCFYEEIAYLINHGLIKGGSLENAVVIRDDA
                     VLTTEPLRYPDEFVRHKILDIVGDLALLGRPLRGHLVAVRPGHAANCELLRRIIAQMR
                     KPVVAAQTFAPPPPAPKTAPPPAAAADETQPQESAIDIQGVMKLLPHRYPFLMVDRVA
                     KIEGSHIIGIKNVSINEAYFQGHFPGHPVMPGVLQLEAIAQVAGIIMMKSAENAGKIA
                     YFMSAEDVKWRKPVLPGDVLVIDVEMTKIRGKIGKAKGVCKVAGEVVSEAAVTFMLAE
                     P"
     gene            23425..24189
                     /gene="lpxA"
                     /locus_tag="PKI07_03655"
     CDS             23425..24189
                     /gene="lpxA"
                     /locus_tag="PKI07_03655"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOC50178.1"
                     /translation="MIHPTALIHPRAQIGPDCEIGPYCVIGEHVELGAGCRLHAHVVV
                     DGHTRLGRQNVIYPFASIGLQTQDLKWKGGVTRTEIGDHNTIRECVTIHSATGDGEAT
                     VIGSHNNILASSHLGHNVVIGNHVIISMAGVAGHVVVEDYALVGGMSGVHQFCRIGKM
                     AIVGGCSRIPQDVAPFMLVEGNPPETRMINKIGLERNGVPAETQAALKQAHKILFREG
                     LTTSNALARIEQELPPLPEVQYLVRFVRASERGIIK"
     gene            24201..24977
                     /gene="rsmI"
                     /locus_tag="PKI07_03660"
     CDS             24201..24977
                     /gene="rsmI"
                     /locus_tag="PKI07_03660"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOC50179.1"
                     /translation="MSAPAQNASADSGWPPLAPGTLYLVATPIGNLEDITLRALRTLR
                     ECDVVAAEDTRRTGRLLKHFGIVKPLLSCFRFNEAQRCGEIIERLQRGEKVALTTDAG
                     SPGISDPGERVVQAVIAAGLRVEPVPGPCALVAALTASGLPATEFHFIGFLPHKSGPR
                     RRELERLRHTSGTLVFYESPYRIGKLLAELNGVFPACQVVLARELTKKYEEYLRGTPA
                     ELLALLRQRSLKGEFVVLLSGEGRPAMEPDMDSDFCGPGD"
     gene            25031..26815
                     /locus_tag="PKI07_03665"
     CDS             25031..26815
                     /locus_tag="PKI07_03665"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HOC50180.1"
                     /translation="MKNNTPLLNYHPLGIYLPTPFRTWSGILRRFLAWAVSIPLAGIS
                     LTASAAIIAIQDFEPIPAVPTFLYTATGGSILTGSSGSGGGPWGSNGDRPSFSPYYSG
                     GAQAYQVNSGTATLTFDAVNTGGFTGVQLSLRVAAFSAATSANGLDLGDYVEVQISPD
                     NGAHYYPTVRVTGNNNAYWSYAGGTGNAATAYDGDATAAVFTPAGKGERTTDGYSTIT
                     ITSLPEVSQLRVRVIMTNDHENERWVIDDLLITGTLAEPPQAGNNGPVCAGETLSLTA
                     STIPSATYAWTGPNGFTANVQNPTIANVTPAASGVYSVTATVNGHTSPAGTTSVIVNP
                     PPATSAISGSSVVAIGQAGQIYSVTPTLGSSYAWTVPGDATITAGAGTATIEVRFGSF
                     GGNVAVSETTAAGCVGDPISLAVAVGPNHAPIAQNKTLTTTKNTPATCFHSKLLAGAT
                     DADHDSLSISSADPTSAQGGIVVLQAGNITYVPPTDFIGSDSYSFTISDGQGGTATAT
                     VSVTVTANNGGAPNVVIPPTYDSANRTFRVTFAGIPHRAYTVEAASEPNGPWSYLKTA
                     TADAYGLLEVTDVLPLSVTARYYRTTSP"
     gene            complement(26964..28505)
                     /locus_tag="PKI07_03670"
     CDS             complement(26964..28505)
                     /locus_tag="PKI07_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOC50181.1"
                     /translation="MKPTYSLTSALFKRLGLKPENPGAFGGEWFGSGKLLKSISPIDG
                     RTLATVRTATPQEYERVARRAQQAFQDWRLVPAPKRGELVRQLGNALRQAKTDLGRLV
                     TLEAGKILPEGEGEVQEMIDICDFAVGLARQLYGLTIASERPRHRMMEQWHPLGVVGI
                     ITAFNFPVAVWAWNSALAAVCGDATLWKPSSQTPLTAIATLKIAERICRANKVDPAIF
                     SLVIGEGATIGERLINDPRVPLISATGSTRMGRHVAELVARRLGRSLLELGGNNAIIV
                     TPSADLDLAMRAILFGAVGTAGQRCTTTRRLILHESIRESLTRRLVGAYQQVRIGNPL
                     KPGTLMGPLINPRAVEDMQAALAAARQEGGQILYGGEPLNGSRYPGGCYVRPCLVTAR
                     HDMKIVHEETFAPILYLIPYRTLDEAFAYHNEVPQGLSSAIFTNDLREAEQFLAAHGS
                     DCGIANVNLGTSGAEIGGAFGGEKETGGGRESGSDAWKNYMRRQTATVNYSKDLPLAQ
                     GIVFG"
     gene            complement(28539..29915)
                     /gene="lat"
                     /locus_tag="PKI07_03675"
     CDS             complement(28539..29915)
                     /gene="lat"
                     /locus_tag="PKI07_03675"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HOC50182.1"
                     /translation="MSVIEQASSIAANPTAGPRASGAVSPQQVIARLEEHILVDGFKL
                     VFDLARSRGSRFVDAVTGRELIDLYSFYASQPIGFNHPYFDQPEVKADLLAAARVKVA
                     NADVYTVQYAAFIQTFARVASLVPLERYFFIEGGALAVENALKAAMDWKVRRNLAAGL
                     GERGTEIIHFERAFHGRSGYTMSLTNTDPRKIAYFAKFPWPRIIAPNLDFSLPPAQRE
                     AAVVEKERQALEQIRDVLARKAPEIAAIIIEPIQGEGGDNHFRGEFLRALRRICDEND
                     VLLIFDEVQSGLGITGKMWCCEHFDVRPDLLVFGKKVQVCGVMAGPRLDEVKDNCFRL
                     PSRINSTWGGNFTDYVRSTHYLRIIEAERLVENARCQGERFLAELQALARRYPVISAV
                     RGRGLMLAFDLPNGATRDAFWKGAYELGLLVVRCGERSIRLRPVLDVKDDIIEAALRI
                     MDQQCRLL"
     gene            30335..30409
                     /locus_tag="PKI07_03680"
     tRNA            30335..30409
                     /locus_tag="PKI07_03680"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30367..30369,aa:Cys,seq:gca)
     gene            30624..31700
                     /locus_tag="PKI07_03685"
     CDS             30624..31700
                     /locus_tag="PKI07_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOC50183.1"
                     /translation="MKRRFILYRRKRGGMFYVEDTETRKQESLGTRDRAEAMSLLNAR
                     NESVRQPQLNLHIAKAYLAGTDSGVSTRTWQNALDAIIESKTGSTKDRWQRAAKEKAM
                     DLIRDRVIIETQGEQLLACLKAGTVSTNVHLRKLHNFCLAMNWLPWPIIPKRLWPEVR
                     FQPKRAITIEEHQLILEREKNPERRSFYELCWHLGGSQTDIANLKAEDIDWGNRTIAY
                     TRQKTGSLAMIHFGEEIEAVLRRLPDTGPLFPYLQSVRCGDRATEFKQRCRGLGIKGV
                     SLHSYRYAWAERAKQCGYPERFAQEALGHNSKAVHRAYARRAQVRLPSLESYERQATD
                     GRIIPLPSVSSKLDRNDHSISAAG"
     gene            complement(31633..32016)
                     /locus_tag="PKI07_03690"
     CDS             complement(31633..32016)
                     /locus_tag="PKI07_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50184.1"
                     /translation="MNETAQQTEFLSWKIAPARLTATQTAWFLGFEPHEVPMLVAAGL
                     LKPLGHPPRNSTKFFATELLEQLRRDEKWLARASDAICSYWRERNARKQKARGRAATQ
                     SAAFNRPLRSSGRSDPASTKPTARE"
     gene            complement(32143..32580)
                     /locus_tag="PKI07_03695"
     CDS             complement(32143..32580)
                     /locus_tag="PKI07_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50185.1"
                     /translation="MRIGRFAGFDLFLRPGFNNAVEVVLRGHNSYGARVTDTALGTIR
                     SLEATVQGFEERAGKLETDIADAHKRGKELETKVGAPFEHEECYQELCRRQSEIEEKL
                     DLTKNQAPSQVDADSLDEGTNKISEQQTPTKPVRHARRTAIHV"
     gene            complement(32601..35630)
                     /locus_tag="PKI07_03700"
     CDS             complement(32601..35630)
                     /locus_tag="PKI07_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOC50186.1"
                     /translation="MRKNFRSFTPDRRWKQGLFWSRFRPINLRANQRAFDGDPDLPLL
                     LSLEHYNDETKRATKATIFTERTIHHRQPIEAVSSPKEALLVSLNERGRVDLDHMAAL
                     LNKPTEEFLPDLKGMIFLNPQTNQWETDDHYLSGNVREKLVVADAAAVTDSRFLENVE
                     ALKSVQPADLNATEIDVRLGASWLPPEDVKQFTQELLNIPSGVEIGHIHALGTWHLTG
                     NWEARGATANTTDWGTNRYTALELIEDALNLKTPTVYDLNEDKKPVVNAEATEAAREK
                     QERIKERFKEWVWADDSRRERLCRLYNDMFNHTRVRTFNGDHLTLPGASGVIQLHRHQ
                     KAGVWRILQTPNTLLAHVVGAGKTYTMVAAAMELKRLGLARKPMFTVPNHMLGQFSTE
                     LLTLYPGANILVAGKEDFESQNRKKLFSRIATGNWDAVIVTHSGFERIPLARETQERF
                     FQEQLHELEMIKRQHADSSNRRLVKEIERAKKRLEARLQALAAEHKKDNTLTFEELGV
                     DRLFVDEAHYFKNLFYVTKMTRIAGLPQTASERAFDMYLKVRHVQSLNGGGGVVFATG
                     TPIANSMAEMFTMQRYLQADQLKKHNLHHFDSWAATFGEPVTAMELSPDGAGYRLNTR
                     FARFINVPELMQMFRQSADVQTAAMLNLPRPLLEGEKPAIRNAPATPELKAFVQELAA
                     RADRLKTGRVDPSEDNMLKITSEGRMAALDLRLMKPATPDEPQGKVNLAVENIHRIWQ
                     ATAAERCAQLVFCDLSTPKDKGFSVYRDMADKLERLGVPTREIAFIQDYDSDASKLAL
                     FREVRSGKVRILFGSTQKMGSGTNVQERLIALHHLDAPWRPADVEQREGRILRQGNKN
                     AVVSVFRYVTEGSFDAYMWQTLETKAKFIAQVMSGDMTIRRVEDLESAALTYAEVKAI
                     ASGNPLVIEKAQVDAELMRLTRLRSAHAEEQYRIRSNLRRAHEDAETFTARLANLRQD
                     IAVRQDTSGEKFTIELDGQTLDNRGIAGELIVRRAEK"
     gene            35909..38509
                     /locus_tag="PKI07_03705"
     CDS             35909..38509
                     /locus_tag="PKI07_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC50187.1"
                     /translation="MKAIKHITGRLLTGCMVVFGLVINLGAQNKPIDKPPIPGTYYSA
                     KDFGFGPPYPFNPHPELETVEVEPGVFVIDDTLIPDTPEQAAARAAHQAAVEQAKAIA
                     SDPILAEAARQAAEEAVRKREQAWQERLQAVQPFLRSWGWPGDAETHQRREEAKAELA
                     ELKRKAEAARANAPQDEAALDEIASRLNVERVRVRTDGGKEVLGGAVGDQPVYYSSQN
                     LFAGASISADELWPTNTAPWAHASTGRNLTGTNVTLGLWEIDGGVLTNHAEFDARVRQ
                     VDNTATNPVSSDAHATGVAGTMGAGGILQFTLNGQPARLLRGVAFESKLNAYTFANFK
                     QERLEAAACSVTGQPLRLSNHSYGASGGWIYTTILVHQGNQTNLVTTWLWRGDPSLTE
                     EWKFGFYTPGAPDIPDGSGCVDTDAFLATNATRHLMVYAAGNDRLTGPGGPTNYYYFS
                     GPGPYDYTQVTNPSANSRDWRNGDGDTGGYDTVLFPGTAKNVLTVGAVRDVYTFSGTN
                     LIMGFATNASIVLAPFSGCGPTDDGRIKPEVVAVGQSNSLLRTFGIATPANNATNGYF
                     IAIAGTSFAAPAVAAGLGLAAQRRLQLFPNLDPDLDAWRGSTWRALAIHTADDVDGAG
                     PDFQMGYGLFNVASCVAQVERDQQHGRGTHIKEFEIGVSNTVSWEVDVDSVPFKATAA
                     WSDPAGPATNVVIVDLTKPMLVNNIDLRVEKVGATNVYYPWILNPDLTNKTESARAAA
                     ATTGVDNRNNVEQVYIANPTPGKYRIVVTHSGGLLGGPVPSAQWVSVLTTGETPVSPV
                     FTSIEASPSGTNVLLTFTADPGAYFHLLSSTDLVAWQTNATVRAEGVTNSVLVSSTQP
                     YEFFRLRRQQ"
     gene            38540..39151
                     /locus_tag="PKI07_03710"
     CDS             38540..39151
                     /locus_tag="PKI07_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOC50188.1"
                     /translation="MVIAAAWLGSPQPSCAQGTIVFNEPATPIPLFTTGFAQYYSFDL
                     DGDSATDFTFTYSFQFLGVQPEGTGRILTWLDPPPNVGGSVAPLPLGFVIGAGSETGN
                     LAWYGHALDYNTLAICFDVGCAGAFVGQHAYMGVEFQHAGATHYGWVLLNVAQAYPSG
                     VIESWAWDTRPSASIFAGAVPEPSTWTLFGIGALAIALLRRRC"
     gene            complement(39213..39620)
                     /locus_tag="PKI07_03715"
     CDS             complement(39213..39620)
                     /locus_tag="PKI07_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50189.1"
                     /translation="MSKPKTSAPSSAGGNAAPTDQPTFRTNPEVDAKIDAYIKDNPKY
                     WNYVQAMPRERLERTVVLNEVRELDRQQRMREGLMKRINTNPALKNAYDTLVKDLPDD
                     QKESVIAQIARSARRAVARSQGQQQTRGEAVAA"
BASE COUNT         7457 a        11940 c        12562 g         7713 t
ORIGIN      
        1 gaaacttcca tggttattgt aatccaaggc cgggagagcg gcccggcggc ccgcgcgcgc
       61 gggtcagtta accgggcagc ggtttgtccc cagccaccgg gaaaagggta tcaggccgga
      121 ggcggcggtc aacgaagata gcaacccgcc ggattcgagc gcgccgcgtt tggctgaggt
      181 gccagccttt ttgtggggct gccgtccgga gggcaaggct ggccggcaga agggacggaa
      241 aaaacaggca gttgctcaag cgcacaggag caaagggagg cggagttccc gggcggaagc
      301 tgcgcgcggc gcgcgaaata acgtcctggc tgtctgcggc tgtggcggcg cgcgggcgat
      361 ggagcggttt gctgccggtg ccgttttgag gccgccggtg ggagggtgtg gcgggtgagg
      421 gcatgaggaa aaggcagttg ccgtccggcg aagggcgggt aatgttttgg ccgagttatg
      481 attccggcag ggcgcagcgt atggcggcga ttgacgccgc gtgaaatccg gatgttgtgc
      541 tacctggggg tgctgctggg agtggcgatc tggagatttg ccccgcgacc gtggcatccg
      601 gcccacaccg tcgaagtgcc gcattatgtc atccagtcct cggccacgga gttgcagacg
      661 gcggaggcgg gtcaggcgtt ggagatgctt tacagcgcct atacgagccg ctttgagggg
      721 tgggcgcagt ttcagccggc gcatccgaag ttgaaggtca ggctgttccg ggatcgggcg
      781 gagtttcgcc ggatcaatcc cggcctgggg tgggccgaag ccttttatcg gaagccatat
      841 tgccaggcct attacgaggc ggcagagatt aatccctgcc atgcgttatt gcatgaggcg
      901 gtgcatcagc tcaacgaaga agtgaccggg gtggcgctgg cccagtggct ggaggaggga
      961 ttggccacct acttttccac cagccggatg atttcgaacc ggctggcggt gggccggata
     1021 gatgtgaata cctacccggt gtggtggttg gacgagttgg ccgtcagccc ccacctggcg
     1081 gagaaccttc gcaacggcag tgtgattccg ttgttggcga ttatcacgaa tcgcggcggc
     1141 cccagcctga accggcgggt caacttgtat tatctgcact ggtggacgct ggcgcatttt
     1201 atttttgagg attcgcagta cggccaacgg gcggggttct tggtgcggca gggcggggga
     1261 ctggagtcgt tcgaagcgac gatcggcccg gtggagaagg tgcaggtgga gtggcatgat
     1321 tatgtccggc ggctcaaggc ggcgtggacc gccggtgacg tgaggttttt cagggacggg
     1381 atacttccgg aaccggcgaa atcatgaggg cgggagagga gggaagggtt ccctgggcgg
     1441 ccacgatgag cggggcgcac gcggcagcga accgccggcg gtcggaggat ggcgcgagtg
     1501 acgaggcggc ggcccggcgc ggaggaatga gcggtggaat tgaccggggt caaggcggcg
     1561 ggaggcggga tgccggaggt tgagcgtggc gatgatgagc acggatgtta cgtcggcggc
     1621 cggtttgggc ccgcgccggg gcgcggggcg gcgggttgcg agccggcggc tggagcgcga
     1681 gtggcggacg attcaggcga tggtgcggct gtattgccgc gcgcatcatg cgggcggccg
     1741 cgagctttgc gcggagtgcc aggggttgct ggcctatgcc gccgtgcggc tggagcgctg
     1801 ccggttcggg gcggaaaaac ccacttgcgc gcgatgcccg gtgcattgtt atcagccagc
     1861 ccggcgggag caggtgcggg cggtgatgcg ctatgcgggg ccgcggatgg tctggcggca
     1921 tccggtgttg agttggtggc attgggtgga tggattgaag cggccggcgg gaggcggacg
     1981 gcggggcagg ggatgagccg gatcgggcgg catttcccgg aggaggagcg ggttcggctg
     2041 ccttgaggcg cccggtggcg ggcgggggtt attcgcggtt gccggggttc aggcgcggcg
     2101 gggtttcgac tggaaaggcc ccgatcatct ggcgcacctc cgttaagacg gtttccacgg
     2161 gaagctgggc caggcccgcc gggtggcgca gcacgatcat tttttcactg ggcggccgcc
     2221 agatttccac cgcggtttca ccccacaaga gcactcccgg gaggcccacg gcggcggcga
     2281 ggtgcgagat gccggaatcg tggccgatga aggcgcggca gccggccagg tggcgcgcca
     2341 gttcggccag gggacggctc tgcgctacgc gcacccgcgc gggcggcagg ggggccgcga
     2401 ggcgcttcag ccggtcgccc tcggcttctc cgcccacgag cagcaactcg aagtccgtgg
     2461 cggtggccag ataatgcaga agctccgccc accgggcttc cggccagttt ttgcgctcgc
     2521 tgccgctgcc gggatggagg gccagggggc cgggcggcgc cgggggcgga ggcaggcgca
     2581 ggcgggggac ggggtcggcg tcgaagatgg ccaggcgttc gagcggctgg agatagactt
     2641 tggcggcgtg caggccgcct ccgtccatgg cccggtgcgg tccggggatg aagtcggcgc
     2701 cggtgcagag ggccacattc gtccggaaaa tgccgtccgg gtcgtagaga taggagacga
     2761 tgacatcgaa tccggcgaag tagctgatga gttcgggggc cagccggccg ccgcgggcga
     2821 aaaagccggc gaggttggcg gcttcaatgg gttgaacccg gttgaccagg ccgcccgcca
     2881 gggccagttg ggcgatgtgg ggatagccga ggatttccag gtgcgcggcg gggaactgcc
     2941 gccggagcga ggcgatcgcc ggcagcgtga gcacgaagtc gccgatggcc ccgccgcgaa
     3001 tcaccaacag tttgccctgg ccaggattca caggcgcaag ggattcgggg aggcggcgcg
     3061 gctcaggaga cggtgcggaa gaccgtcagc cccagggccg ccacgaacag gccgaacgcc
     3121 agccccaggc tgcaggccac ctgggtgcgc tgcggggttg cggtgcccca ggccaggaaa
     3181 tcgcgcaggc gccagggcga aatggtgagc cagatgccgg cgaggaccag cccataggcc
     3241 caggtttggt tgattaagac ccagtgcgtc tggctcagca gggggcggcc gctgtccacc
     3301 atcagtttgg ccagcaggag cagcacgatg gccagcccgc gcacggccag gaagtcctgc
     3361 acgaatatgc aggcgcccag gccgatggcc gcaaagccga cgaacaggtg gctcttgaaa
     3421 cgggcgaaat cggcgatgga ctcctggctc aggttccaca ggaaccaggc cgtgcccagg
     3481 gccatcaggg ccagccccca cggcagcgag cgcgggaact tccgcaccgc cgcggcaaag
     3541 gccgcgggtt tggccaggcc gtagaggagc ggcagcccca cgcccaaacc gagcaacagg
     3601 ctcaaggtcg agagtttcaa agtcatttca gcaaccgtgc ggaggatggg cgataagccc
     3661 ccgcggcgca agctgggaat ggcggcgttt cgctactgtt cccgcggggg gggggccggt
     3721 ttggccgtag agtgtgaatg tgcccgcgtg cgagatgcgc acgtccagga gctggccgcg
     3781 gcagtgcggc gggccctcga atacgacgat cttattgcag cgcgtgcggc cggtcaggcg
     3841 ggaagggttc tttttgctcg gcccttccac caggacctgg accgcccggc caaccagctt
     3901 ctggtgctgg cgcgcgccgg actcattgac ggcggccagc agccgctggt tgcgctcctc
     3961 ttttaccgcc tggctgattt gcccgggcat ttgcgccgcg ggcgtgtcgc gccgcggcga
     4021 gtatttgaac acgaacgcct ggtcgagctc ggcttcgcgc acgagccgga gggtttcctc
     4081 gaagtccgcc tcggtttccc cggggaagcc ggcgatgaag tcggtgctga tgccgatggt
     4141 gggctgcacg cggcgcagtt tcccgatgat ttccaggaac cgctcgcggg tatagccgcg
     4201 gtgcatccgt ttcagcacgg agttgctgcc gctttgcacc gggatatggg cgttttccac
     4261 cagtttgccg agccgcccat aggcttccac caggtcatcc gtgtaccctt tcgggtgggg
     4321 cgagctaaag cggatccgct ccaggtcgtt gatttcattc accgcttcga gcagttgcac
     4381 gaagggggtg cggccgtcgc ggcgggggat gaggccccgg ccgtagctgg tgacgatttg
     4441 gcccaggagg gtgatttcct tgacgccctg cgcggccagt tcgcggcatt cggccacgat
     4501 atcgcggatg gtccggctgc gttcctggcc gcgggtgtgg gggacgatgc agaaggtgca
     4561 gaattggttg cagccctgca tgatgctcac ataggcggtc acggatttgc gggcgttgcc
     4621 gtcgagcaaa tgctcgcgaa tcgcggcttc actgcctgct tccgccgcca cgtccacgac
     4681 tttcccgcgc tggccggtga aaaggcggtc gaggtgctcc gcggtgcgat gcagcttttg
     4741 cgtgccgaca atcagatcca catccggcag gcggtggatc agccacgagc cccggttctg
     4801 ggcgacgcag cccaggaaac ccagcaccac ccgggagcgg cggcggcggg tttccgccac
     4861 gaggttttcc attttgcgca gcgctttctg ctcggctaaa tcgcgcacgc tgcacgtgtt
     4921 gagcaggatg acatcggccg atgcctcgga gggggcgagc gaatagccct gggcgacgag
     4981 ttgcgcggcg accgcttcgc tgtcgcgctc gttcatctgg cacccgtaag tcttgatata
     5041 aacgctcggc ataggggctc ggcgagtttg atcaaattaa gccgcccgcg cggggttgaa
     5101 aaggtggaag tggccgggtt agcggggagt ttctgattcc cgccggttca gcagccagcc
     5161 cagcaaggtg aaggcgggcc atgcgagcgc cgggatgtaa agcccgaact catagaggct
     5221 ctgcaagctc cagcccagca cgcccagcca ggcggcgaat gcgagcgcgt attccggccc
     5281 ggccgggcgt ttgggccgcg cccgggggaa tccccagacc agcgcgccga ggataaaccc
     5341 ggtgtaggcc agcaggccgg cgattccgga atcggaagcc tgctgcaagt agtcgttatg
     5401 tgccaggcgc gccatttcgg attcgggccg tttgacctgt tgataggcga tggcgaatgt
     5461 gcccggcccg gtgccgagcc aggggtgggc gcggactgtt tgggcggcgg cgcgccagta
     5521 gtcgaaccgg gcgaccacgc tcgttgcccc tttccgaaaa aaggaggcgt atttccagaa
     5581 gaagccggcc agtccggcca gcaggatcgc ggccacgagc agggttttga gccgccggct
     5641 gaatggcacg cgcagcaaag ccagcaggcc cagcaggagc atcagcagcc agccgccttt
     5701 cgagccggac caatacaggc aggccagccc ggccatcccc gccaggctta tcagaaaggc
     5761 gcgcgcggcc ggggtcagaa acgcgcgcag ttgtccgagg gcggtgagga gcaccggcag
     5821 cagcagcagc aaaaccccgg ccagggtgtt gggataaaac agcgtgccaa agatgcgatc
     5881 gctggtgatt cgcttcaggt agtcggggga tacggtttcc aggtggggat acacgtagag
     5941 gaagaaatac tggcgcgatt gccggagtcc gccccaatgc tgctcccagc cgaccgccag
     6001 gaccagcagc agcgccccga gcaagccaat ccagaatagc caggcctgct tgacgcggcg
     6061 gagggagaag tagccaaggt aaaaacaaag cacgcacgcg ccgaagtgtt tgagggtggc
     6121 ggtggagagg ctcggatcgg cggacgcggt ggcggcgagt ccctgccagc ccagccagat
     6181 cagcggcagg gcgatcagcc agccgggcgc gggggaccgc cagtgcgcca ccgccgcgcc
     6241 caggacggcg accccggcca gcatcccata cgcccaggcg atgggccacg gatagccgaa
     6301 caggaactca tatatcccct ccggggggac cacccactgt tcggtgatgg gcgggttgcc
     6361 aaactttaac agcgccaggc cgaggaatgc gccccacagg gcggcaaaga gagccgtcgg
     6421 ccacccggcc cgcggggtct tggcggctgg cgcggggaag ctcaagctac aggtggagtt
     6481 tcagcagccc gacctccagg gccagcgctt cctggacgtt ggtctggagg taacgctgga
     6541 gttgttcgag gatgcacagg ttctcccgtg ccgctttggg cgaaagccgg cgcgccaccg
     6601 ggtgcgtccc cgccagttga gggaaagcca gcagggagcg gaggctggct tgggagccgg
     6661 cggattcccc cagggacaag agccagacat cccgcagcca ccattggagg gccaacagaa
     6721 attcaccccg ctgacggcgg tattcggctt ccaccgcggc gttaagctcc agcgtccatt
     6781 ggtcgcgcag cgcggcgtcc acctcctcgt atttctccag cggggaacga gcgttgaggt
     6841 ctttttcgat gtcgtcccgg acggttttca gtttgcggag cagcacatcc atgaggcggt
     6901 agcggcccaa caggcttttc tggctggcgg ccgccatttc actgaacgcc gtcagccatt
     6961 cgagttgcgc cgccgcccag gctggggggc cttcgccggc aaaattcaag cgcaggcagc
     7021 gggagcggat ggtttccaat aaacgctccg gctcggtcgt gagcagaatc agcaccgaat
     7081 gcggcggcgg ctcttcgagg gttttgagaa aggcgttggc cgcctcctcc gtcatgcggt
     7141 cggcggccac gatgatgccc accttgtatt ggctttcggt gggcttgagg ttcaccacgt
     7201 ccagcagcac gcggggcggg ctgttgtcgc gatggacaat ctggccgatt ttgatttgcc
     7261 gcagcttgga ttcggggcgc acccagaaca cgtcggcatg ctggccgtcg cgcgccttcc
     7321 ggcagccgga gcagcggtcg cagcaatcca ccgccgcgcc gttgcgccga acggggtgct
     7381 ggcagttcaa ggtttgggcc agggtgcgcg ccaaggcttc cagggaatcg agatgggggc
     7441 cggcaaacag gtaagcgtgc gccagccgct ggcgctcgag tgagcgttgc agcagctcca
     7501 cgccttgcgc ttgcgcggga aaatctttga atgccatccg ctctgttata aatccccggc
     7561 tgcgggaagg gaagcgggca ttttaacggg agttgacggc gggggggctc agcgcttaaa
     7621 cccttgccat ggaatgcaaa aagtcgcgca acgcacagtt ctgcaattgc acctacccgg
     7681 gctgtgaaaa gcatggcatc tgctgcgagt gcctgcaata tcacctgggc atgcggcagc
     7741 ttcccggctg ctgttttccc aaagaggtcg agcgcacgta cgaccggagc ttcgaacgtt
     7801 ttgccgccct ggtgaacgcg ggccaggtct agcgggcgtt ttccggcgcc tccgcttttc
     7861 aagcgccttc cggggtttat tccaaccggc atctccgagc gcctcatctg gcgcgccggg
     7921 attgaggacg cccggccggg ggctgtccgg ctgcggccag taaagcggca gtggctctgg
     7981 cggcgcattt tttccggagg aacgggcgcg gccgcggcca ttcccggccg gcaagctctc
     8041 aagggttaac gggtcaacgg gatgcgcgcg gaagagttct tgctccgcag cgccttttcc
     8101 ggtcaggatg gctgcgcata atgaagataa agggactccg ctggtacatc gcgggactgc
     8161 tatgcctggc ttcggcgctg aactatttgg atcggcaaac cctttccgtg ctgattggca
     8221 ccattaagga ggaactcagc ctgacgaacg ccagctatgg cgccattaac gcgtggttcc
     8281 tggccagcta cggggtgatg tacgccgtca gcgggcgcat catagactac attggcacgc
     8341 gccggggatt cgtgctgttt gtgtcgggtt ggtcgctggc caacatcctc catgtggtgg
     8401 ctgccaccgt gggccagttt tcgttctttc gctttctgct gggagtcttc gagccgggca
     8461 gcttcacggg cggagtgaaa gcggtcggcg agtggtttcc catgcgcgac cgcgcgctgg
     8521 ccattggcat tttcaatgcg ggtacggcgg tgggttcggt ggcggcggcc ccgatcgtgt
     8581 ctttcattgc ggtcacgtgg ggctggcgat cagctttcct ggtcaccggg gcgctgggat
     8641 ttgtctggct ggtgccgtgg ctgctgttct tccgccagcc caaaacgcat ccgcagctta
     8701 gcgaatcgga actccggttg attctggagg gccagccgga ggaaacggcg ccgccgccgc
     8761 cgccgcggct ccggcaattg ctgcaaatgc gcgaaacctg gggttgcgtg ctggtgcgcg
     8821 cgctgaccga cccgatcagt tactttctcc tgttttggat cccgctctac tttcaaaagc
     8881 aacacgggtt ttgcctgcaa cgcatcggcc tgttcatttg gattccctat gccgcggcgg
     8941 cgttgggcaa cctttgcggc ggcatcatcc cgcgaacgct cattggccgg ggctggtcgt
     9001 tggacaaagc gcgcaagggt acgatgatct ttctgacgtt cacgctcctg gtctgtttgt
     9061 ttgccgtgac ccggacggct aatccgatgg ccgcgctcct gctcgtggcg ggcatgacct
     9121 tctgtcatgg tggctggggc aacatcaccc tgccggccga ggttttcccg aaaagcgcca
     9181 tcggcacggt caccggcttg ggcggggcgc tgggctcctg ggtgggagcc ctggcgatgc
     9241 tgagtacggg ccatgtggtg gatgcctatg gctttacccc catctttatt acctgcgcgc
     9301 tcttttaccc gctggcgctg ctgctggtgt acgtgcttat tggcaagctc ggggtggttc
     9361 gggccctccc gcaatgaaag gaaagggcgg cgcgtttggc tggagaatca aaggggcgca
     9421 tccggcctgg ggccggccgc ggccgggaag cgaaggaaac gcgccgcggc gtcaacatcc
     9481 ttgagcccgt ggccggtgat caggagcacg atttgctccc ggcggcccag caagtccgtg
     9541 ccctgaagcc ggcgcagggc cgccacggtc gcggtggctg cgggttcggc gaacacaccg
     9601 gtcttctccg ctagcgtccg ttgtccccgc actatctccc ggtcggtcac tatgagggag
     9661 aatccccggc tttcctggac cgcccgccgc gccaggtgcg cgttggccgg aacggaaacg
     9721 gaaatcgagt cggcaatcgt ggcgggtcgc cgggcgttct ggtatttgcc cgtgcggatg
     9781 taatgatgga tggccgccga gcgttccgcc tgcacgcaga tcaagcgcgg caaccgggca
     9841 gtcagcccgg cggctttcag atcgaggaaa gccttgtgca ccccgctgat gatgacgccg
     9901 tcgccgaccg gcaccagaat cgcatccggg accttaaacc ggttttgctc atagatttcc
     9961 agtcccacgg tctttttccc ctcaagggtc aggggatgat aggcggtatt ccggttgagg
    10021 ccgcccttgt gcgcggtgta ttccagcgaa agctggaacg cgtcatcgta ggtgccgcgc
    10081 acgggaatga cgcgggcgcc atacaatacc atttgcacga gcttggcctt cggcgcgctt
    10141 tcgggcacga agatgatcgc ctgccggcct gcggctgcgc agagcgagca gccaatacac
    10201 agggccgcgt cgaagcacgg caccttgttc cggttgaggg tgatggcctg atacgggcag
    10261 cgcgtgcaat tgccgcaatg ttcacacagt tcagcgtcca cctgcggcaa cttcttggtc
    10321 gccggagagc cataaagtcg gtaatcggat gcggcagcgc cgcgccgatc aattctttga
    10381 cggacttgaa tccccggtcc tgcatgagaa aactgagccc ggagtgcaat tcattcacga
    10441 tgcccagccc gtatttcatc acgatggtgc aaaactgcac cgtttgggcg cccagggcca
    10501 ggaagttggc cgccgccttg tagttcatcg ggccgccatt gctggacacc gcgatgccca
    10561 tgcccgacac tttcgccagc gtcaagttgc tgatcggcag caccccttcg ccggacatgc
    10621 cgaccaccac cccctcttcc caacgccggt cctcggcccg ccggagggcc agtgacggga
    10681 agctgttggc cagggtgacc ccggctttct tatgaggata ctgggcgaaa acctgctgga
    10741 tgtactggat gatcggctga atggcggtga ctgcggcggt gagcttgaac agtttcgggt
    10801 tatccgccga gctggcttcc atgacccagc cgataatttt ggccgccaac tccgcgttct
    10861 gagaaacaat gtcgccctgc gctccatcgc cgccttgggg gcaagaaagg ctgtattcaa
    10921 cacccatggc tcccgccgct tccagttttc gcgtattcga ctgccaaaca gccttgtcgg
    10981 cctgatcctg tcccgtgacc ggtccgccgg tcgaggccag tgcgagccgg tccgggaatt
    11041 cccgcaccaa ctgccttact tcccggcaaa cacggtcgag ttcatgaccg gagacattgt
    11101 cgcagttggc ataggtcgaa ggacccaaca cggtcatgta ttcggaggga atatggatcg
    11161 gcaccttatc aaaggcggtc ttcatcaccc cgccggccca acccgccgcg tatgccttgc
    11221 gcatttgggc ataaccgtcc gtatgcggcg ccgccgagag cagaagggcg atcgaatctt
    11281 acgcccgaag aactcggttt cgagcggcac gggatacagt tccaagcccg acagcacgac
    11341 atggctcttg gcgcggttct tgaggcgagg cggctttttg cccagcagat aggcctcggc
    11401 ctccaaagcg gcgttcttgc cggtagccac tgactcgaca accgtcgccg agcctaaaac
    11461 catttcgccg gcgtaaaagg cgccccgcac ttttcggatt ggcagcgagg atcggcttct
    11521 ggtggcgatg ataaccacat caaactcccg gaagatcggg gattctctcc ggttgaccac
    11581 gaagttctgc ggccggggcg ctttgccctg cgggagcatc atccgggcga ggcgcaaccc
    11641 ggttgccttc tggcctcgat gcaagatctc gaggggcttg cagcaactgg tgatttcgat
    11701 gccctgctcc agcagtatct ctcgctcgta agcggtcagc ggcatatctt gctgccggcg
    11761 ccggtaaacc aactccaccg ataacgcgcc gtgctttctg gccgtcatgg cacagtcaac
    11821 agcgaccgcg ccgccgccca gcacggcgac cttccgtccg gctaccgcga tctgcttctg
    11881 ccgttccaaa tactcctgcc aggaaagcgc gtattcctcg cccgggatgc ccagccgcat
    11941 cggctcttcc agtccggcgc tgacgatgac cgccttgtag tccttgagca gcgcagccgg
    12001 atcggttatg ccttggccgg gtcggaaatc aacggcgcca agggccttga taaactcgat
    12061 atcccggttc gcgacttgcg ggtcgagccg gtcattcggg atgagccgca tcatactgcc
    12121 cagccgcttc cattgttcgt acacggtcac ttggtagccg cgctgcgcga gcacgctggc
    12181 ggcgccgaga ctcgccggcc ccacaccgat taccgcaacc tttttgccgt tgcattaagg
    12241 tgccgggaaa cttcctaatc ccgcttagtg gcgcgcttca tcacgctggc ctgaaccgcg
    12301 ggaatattga tcggctggtc aaacgtgcgg cgcacacatg ccttcatgca gaagtaatcc
    12361 cggacagacg actccgcaaa cccagcccag gggattcgct cccatgatca gcgcccccgc
    12421 ccgccggtaa tcagaaggcg ctcccatgcg agccgcaatg atgaagtccg ccagcgagca
    12481 atccgccggg caggcgacct gacatggctt ttcttcgcag tattcgcacc ggttaagctc
    12541 cgcctgcaat tgggcggcgg taagtgatcc gtcgttgttc ttcatggcca ttttaccgac
    12601 agcaagggct ctcataatga cactccccgc gaaagaccgt aaccgcactt cggttggccc
    12661 ttgctgacca tcggttgact ctgctcgagc gggcaatccg cattcatttc ctcacccccg
    12721 ccgactcgcg ccggcgcggt tatccatcct caaccgcttt ggttgcctcg atgaaggttt
    12781 tgacccgggc gtgatccttc tttccggggg cggcttcgac cccgctcgat acatccacgc
    12841 cataaggccg cgcgcactgt atcgcctcgg cgacgttttc gggggacagt ccccccgcca
    12901 gaaagattgg gcgcccccag tcgcgggcct gcctggcgag attccagttg aatgtttcgc
    12961 cagtgccccc cggcaggcct ggcgtgtagg catcgaacag ccaggcatct gtgcgataag
    13021 cccgcacggc ctccagcgat gcccgatcgc gcacgcgaaa ggccttcatg ctcatcaggc
    13081 caaatcccag gcaatactcc ggagactcgt cgccatgaaa ctgcagcagg tttagcccgc
    13141 attcccgggc ggcgtgcaga accagcccca ccggcgcatt cacaaagacc cccactttga
    13201 tcaccgacgc cggcaatttg cgaatgacac tggcagccgc gtccacggaa atatggcgcg
    13261 ggctgggctc gtagaatacg aaaccgagcg catccgcgcc cgcttcggcc gcggccagtc
    13321 cgtcggcctc attcgtgatg ccgcagattt tgacttttac gggcatgctg ttgcggcagc
    13381 atacccgctt tcccgcttgg gacaaccgcg taatgcgctg cgcgcggctt ttcgtatctc
    13441 gaaaagggag cagttgcgtg cgccgttcat acccaccgcc agccctccgc cacgccggca
    13501 cgttcgcccg aaagaccatt tgcgcccgga tttactcagg cctatactgc cgcttctggc
    13561 tgcccccata gctcaaatgg atagagcggc ggtttcctaa accgttgatc cctgttcaat
    13621 tcagggtggg ggtgccagct tgcgcgcgat gccgggatcg gccccttacc agttcaccgg
    13681 cacggtcaat tttcgcccaa agcgattgtg gtaatgcagt tggcgctgct gcgcgccgta
    13741 ttcgtgcacg agccgggtga gtttcacgtc gtagcagcag tactccgcga tttcgagcag
    13801 cttgccttcc cggtaccatt ggatggcttg cagcccctcc gcggtcttct tcaggccaaa
    13861 ggtggccgcg gcgattgcct ccagcgagag ccggtggcgc agggtctctt gcagcttgag
    13921 catcatgtcc agcgtgggca attggcgcag atcaaacgcg gtgtaaccat gtagcacctc
    13981 ataatcgaac cgcagaatgt tgaagccgac gacctgatcg gcgcgctgca attccatgat
    14041 cagttcgttg acccgctttt cgcagtagat ctcgtaagcg ccccgcgcgg tgctgaaggt
    14101 cacgccgacg ctcatgccca tgcggctgat gttttcccag ccgccgacct catcggctga
    14161 tttctgggtc tccaggtcaa aataaacaat gttcttcatc gaggcgtctt gcggcggcga
    14221 caaagccagc ctgccaccgg gccactctag cggacacgga aaagcgggcc taggaaaaac
    14281 gatcggggac aacagtttcc ctccccaatt tccatttttc tgccattgac ttccccacgg
    14341 cgcgcggcaa aagatgtgcc gcatgaaaga aactgctcat ccgcttacgt ctcgtcgcga
    14401 gtttatcaaa accaccggtc gcttcaccgc cgcttccgcc ctggcgggcg tggcgttgcc
    14461 acatgtccac gccgccggga gtgatctcat ccaactggcc ctggtcggct gtggaggccg
    14521 gggcacgggc gctgccaata atgcgctctc cacccaggcg ggcccactca agctcgtagc
    14581 catggcggat gtctttgaaa agaggcttgc cgacagttat gacaacgtaa cgaactcgaa
    14641 gcgagacctg gtggacgtgc cgccgagccg ccgattcacg gagttcaccg gctatcagca
    14701 ggcgatggac tgcctcaagc ccggggatat cgtgatcctg accacgccgc cggccttccg
    14761 ctgggtgcat ttcgcctacg ccatcaaaaa ggggttgaac gtgttcatgg aaaaaccggt
    14821 caccgtagat ggcccgacca gccgccggat gctcaagctg gccgaggatg ccgccgcccg
    14881 gaacttgaaa gtcggggtgg ggctgatgtc ccgtcattgc cgggcgcttc aggaattacg
    14941 ccagcgcatt caaaacggcg aaatcggcga tgtcattctc atgcgcggct accggatgca
    15001 cggtccggtc gggcatgctt tccaaaccaa atggccgggc gaccccagcg aattgctctg
    15061 gcaagtgcgg catttccacg ccttcctttg ggccagcggc ggctgtttca gcgacttcta
    15121 catccaccat attgatcacc tttgctggct caagaatgcc tggccgatca aagcccaggc
    15181 gctcggcggg cggcactaca agcagaatcc cgaaggcgtg ccctacgtag atcagaactt
    15241 cgattcgtat tcggtggagt acacctttgc cgatggcagc aagctataca tggatggacg
    15301 ttgcatgaat ggttgcaatg acatttactc cagctatgtg catggcagca aaggcatggc
    15361 cattgccgcc aaagccggcg acttccaggg gccttccagc atcttcaaag gccagaatgc
    15421 gcaacgctcc aacctcctct ggacttccaa gcctcctcct ggccagacgg acgacccgta
    15481 tcaaaatgaa tgggatgatc tcatggccgc catccgcaac aatcagccct acaacgaagc
    15541 caaatacggc gtcgaagcca gcctggtcac cagcatgggc cgcatggccg cccacaccgg
    15601 ccaggaaatt tcctatgaag acatgctcaa ctgcgagcat gaattcgcgc ccgatgtgga
    15661 taagttcacc atgaattcgc ccgccccgtt gcccgcggga ccggatggca agtatcccat
    15721 gcccatgccg gggatcacca agcagcgcga atattgattt cagcctggcc ctggctccga
    15781 ttgggggctt tgccagcggg cgtccggcgg gtctgaacgt cttggggaag ctcggccagg
    15841 cgccgccgca gccgcccagg cggacgcgga ggatggggga accgggctgg cctgggcggg
    15901 agagggcgtc tttgatatgg agcccgcctt gccgcccgct ttcggggcgc cgggatggtt
    15961 gccgcatgcc ccgcggggct ttggcgctgc cagaaatgga ggcgtgccct tgcgccgcga
    16021 tggcaatatt ccgcttccct caaccgaaac tcctgctaat gatggctcgg tttcatttga
    16081 cgcgcgaatc atgaaagaca ttgttccagc tccgccgccc gtcgtgcgca atgatgtggc
    16141 ggcggtacag acgcttttcc agcagaacgt cgtgccgagc tatggccgct tcgatttggt
    16201 gctaagccac ggagcgggat gcagcctcta cgacatcacc ggccggcgct atctcgattt
    16261 gggcggcggc atcgccgtca actcgctggg ccatgcgcat ccggccatca cggaggcgct
    16321 ggccgagcaa tcgcgcaaaa ttatctactg ctcgaacttc tactaccacg aaccgcaagg
    16381 ccggctggcg caggcgctgg tcagcctgat tggcccgggc aaatgcttct tttgcaatag
    16441 tggcgtcgag gccaacgagg gcctgttcaa actggcccgc aagttcgggc acgatgaggg
    16501 gcgcttcgag attctgacca ccttgaactc gtttcatggc cggacgctgg ccgccatcgc
    16561 cgccaccggg caggagaagg tcaaaaaagg gttcgcgcca gcggtgccgg gttttcgcca
    16621 tgtccccttc aacgacctga aagccatgcg cgaggcgatc tcgcccgcga cggcggcgat
    16681 tatgattgaa ggcatccagg gggagggggg cgtgctgccc gccacgccgg attatctgct
    16741 gggcttgcgc cgcttgtgcg acgaaaagaa gctgctgctg cttatggaca gcgtgcaatg
    16801 cgggcacttt cgcagcggcc gcttccagag ctatcagcgc atcctcgaag cggtgccggg
    16861 cggcgagacg ttcctgccgg atggcatctc gatggccaag tctctggggg gcggctttcc
    16921 catcggcgcg ttctgggtgc gggcgcccta tgcggatttg ctgagcgcgg gctcgcacgg
    16981 caccacttac ggcggctcgc cgctggcgtg cgccgtggcg ctgaagatcc ttgaggtcat
    17041 cgcgcgcgag aacctcgccg ccaatgcgcg ccaggtgggc gcgcaattca aaagccgcct
    17101 ggaggagctt gcccgcaaat acccggcgat cattcaaaag gtgcgcggcc tgggactgat
    17161 gctggggatg gagctggcgc cggccatgcc tcaattgccc ggcgaccccg cgaagacgcc
    17221 ggcggtccgt ttcattaatc tcctgcacgc cgccgggttg ctggccattc cggccggcac
    17281 ccacgtcatc cggtttttgc cgccgctcaa cctccggcct gcggaagccg aggaagggct
    17341 aaagatgctt gaatccgtgc tcgccaatct ggtatgagag gcttttctcc gggcaggcag
    17401 cacgcctgtc cgctttggat atgagacatc tattgagcat tgagaagctg gctcgcgcgg
    17461 agatggacgc catcctggca ggggtggcgc cgctcaaaaa ggaacgcggc cggcatacac
    17521 ggttcccgct ggccggtcag acgtgggcca tgatcttttc gaaatcctcc acacgcaccc
    17581 gcgtttcctt cgaggtcggc atccgcgaat taggcgggca ggtcattttc cttcccgccc
    17641 atgaaatcca gcttggccgc ggtgagcccg tcaaagacat ggcgcgcgtc atgggccgca
    17701 tggtccacgg cgcgatcttc cgcaccttcg cccagcagga cgtggaagag tttgcgcact
    17761 acgcccaaat ccccaccatc aacgcgctca ccgaccagga acatccctgc cagatcctga
    17821 cggacatctt tacgttccag gaaaagcgcg gaccgatcca gggcaaggta atcagtttca
    17881 tcggcgacgg cgccagcaac gtcgccacct cgttggtctt tgccgcgggc aaggccggat
    17941 tcgagttgcg cctcgccgcg ccccggggct ttcaaccgga cgccggcctg gtcaaacggg
    18001 cgggaggaaa gatcgtcgtc accgaagacg ttaaggctgc ggcggcgggc gctgacctgc
    18061 tttacaccga tgtgtgggtc tcgatggggc gggagcagga atccgctgaa cgcctgcagc
    18121 aactgagcgg ctatcaggtc aacgccggca ttgtccggct ggcccggccc gacgccttgg
    18181 tcatgcattg cctgcccgct tatcgcggca aggaaattga tgaggagact ttcgaggccc
    18241 acgcccaaac catctttgat caagccgaaa accgcctcca cgtccagaaa gccattctaa
    18301 actggatgac cggctgacaa gccccctatt cgcagaggcc cgtgcgcgga tcgcgatcgt
    18361 cccgcccgtg cgccagccgt tgctggccag acggatccgc tgagaatact tggagcccga
    18421 ggctgccttc ttccgcccat ctgctccagc cggaccggcg ctccgccagc tcatttcccc
    18481 caagcttccc gggagtgccc cctccagatg ctccccgccc gctttcgccg gttccccaac
    18541 gatttgagtg gaacgagccg tagggcaggc ggagtttgcc ggccacccga tggagggtgg
    18601 taatgcggtt gacgatggcg cggtagccgc aagcctttct tttgacggtg ctgaacccgc
    18661 gattgagcct ttcggtgtgg ttctggcaga acattcgccc gtcatcctga cggcggcggg
    18721 ccgatcccaa aaacgagggc cgctcccgga acttcagaaa caccgtgccg ctggcaggtt
    18781 caaatcagtt cgcggccacc tcccaattca atcccggtcc aaacagctca tgaaacaagg
    18841 ctcccggcgt caggccccaa gccgacatgg cctcctggcc catcttaccg acttcgtggg
    18901 caaatccccg ttttatacgt aacccccgtc tttccagcct catccatagc taccctcaag
    18961 tcgtgtacaa ggggtgtaca agggatgccc accgggtgca caacgaaaaa tcctatgtac
    19021 ttccggtgag catctcttgt acaccccttg tacacggctt ggtggcaggg tggaagtgac
    19081 gtaattgcag ggagttgagg tgctttgaga ggattgggag tccgaggggc tttgggggcg
    19141 gtttttgggg cggttttcgt ggttgaaaca gcggaactgt ggggggaggg aaagcctgca
    19201 ctccttcgcc cttgagccct ggcaaaccgg attccccttt gcccgcgcac tgatcgggcg
    19261 gcgccatcat cgcacggcca aaaagagcgg ccaaacgccc ccgggatcgc acggctgcct
    19321 ttccagcgcc ccgccggagg ctggcggctg tttccgtggc gccagctcat ccgtggccat
    19381 tgggcggggg tgggaatccg caattattgg caccggatag ggcggatggg cgaggacgtc
    19441 tcgcgcagtc gcaaccccca ccggctggct aacctggccc ggttgcaccg cgcactgctg
    19501 gccctgctgg cgcaccgcag ccccgatccg ccccttcccc caaacatcga agcgtttaac
    19561 cgccgtccaa tccgccgcct caacctccgc agctcttgat ctacaaaacc aaagcccctg
    19621 acctattcgg gcaccggagg cattcatgtc tggcggcggg caggctgccg cataaagggg
    19681 gatggcgttg aggatgctcg tgagaggggc aggggagggg gcctatgccc gaagaagaga
    19741 gacgcggaag agcgggcctc agcggacctt ttgcccggcc caatcgcgcg gggcggagcc
    19801 agaggcgaag gattcctggc tgtggagcca gcgttccttg ggttttgaca ccacggagca
    19861 aagctctatg cttaggccgt gcgcattgta agggtatcgc taggccaacg cagctataat
    19921 atccagatcg gcacgggctt gctggctcgt gtggggaacc tgtgccggcg gctggaactg
    19981 gggcgtcgtt gtgccattat ttccgacgcg aatgttgcgc cccgctatgc gcatgccacg
    20041 caaaaggcat tggaccgcgc gggctttgcc acggaattga tcaccatccc cgccggcgag
    20101 acggccaaga gcctgggcac ggtggcggcg tgctataatc aactggcggg gcatcggctc
    20161 gaacgcagct cctttgttgt cgcgctgggg ggcggcgtgg tgggggacct ggcgggattc
    20221 gtcgcggcga cgtatttgcg cgggattccg ttcgtgcaga ttcccaccac tttgctggcg
    20281 caggtggaca gctcggtcgg cggcaaggtg gggttgaacc tcaaggccgg caagaatctg
    20341 gtgggcgcgt tccaccaacc gcggctggtg ctgtgcgatc tctgcacgct ggcctcgctg
    20401 ccgccgcgcg aatatctcgc cggactcgcg gaggtcatta aatacggcat catttacgac
    20461 gccgcgctct ttcgcgtgtt ggagcgcgac ctgccccggc tcttgcggcg cgagccggcg
    20521 tgcctggcgg gcgtcattgc ccgctgctgc gagatcaagg ccgccgtcgt gcagcaggac
    20581 gaaaccgaaa gcggcctgcg cgccatcctt aattttggcc acaccatcgg ccatgcgctc
    20641 gaagcgatct cgcgctacgg ccaatacctg cacggggaag ccatcgccat cggccaggtg
    20701 gccgcggcgc aaatctcggt ttgcttgcgg ggtttgccgg tgtcagaggc gctgcgcatc
    20761 gagcttctct ttcagcgcgc ggggttgccc acccgcgtaa agctcactgc cgcccggcag
    20821 cgcaagctct tcgcggccat gaaactggac aagaaagtca gccgcgggga ggttcgcttc
    20881 gtcctcacgc cgcgaatcgg cgttgctgag ttcgggcacc cggtcccggc gggcatcctc
    20941 cagcgcgtct tgaatcctga accgggcgcc gcgcccgggt tagagcaagg gcaagagtgc
    21001 ccgccacggc cctccggcca ggccgaaggc ggatcagcat gcccgcgccc cggcgttgcg
    21061 gcggcgcatt cccctcaccc cacaccctcc cacgtttaac atgtctgcgg tcagcgaaac
    21121 ccttgttcgc gaatacttcg agttgcacga attcctcgtt cgccagcatc gcaaacatgt
    21181 cggtcaaacc cggcggaacg aagatgacga tattgacttt ttcgtgctta atccgcatcc
    21241 caagcctgct atgggagagc tgccctttgt gctcggctcg cccgagttgt cctttatcga
    21301 gcgcgcgatt gtagtggtca aaggctggca tacggacacc ttcagtccgg cgcggctgga
    21361 agcctcgccc ggcatcttcc gttttgtgga accgcgggtc tttcggcagg cggcccgcgc
    21421 ctttggcaaa gaggacacgc cgctgaaggt gctggtggtt cccgccctgc cgcagactgc
    21481 ggcggcccgg gacgagagca ttgcgctgct gcgttccaag ggcattgacg cggtaatccc
    21541 cttccggacg atgctggcgg atttgattag caaaaccgcc cccaatttca actaccagaa
    21601 gtcggacgtg ctccagctta ttcgcatcct caaaaactac gatttcttca aagagccgca
    21661 gatggaattg ttcaagcccc ggcccggtaa aatcagaaag ggctagtggg aaatctccgg
    21721 cggcaaccgg gaggctaatc ggaagcccgg cgcaaagccc cggggaacgg ccgcgttggc
    21781 ggaacccaca aagctggggc tgcagtttct ggcggtcgtc ttcggttttc tgaggcgcgt
    21841 ctcagtttcg gagcggctcg tttccaatca aaggcatctg aaaaacagtt gaccaccggg
    21901 gataacgttg ccgcgccgcg cccccgggag gcctggagca cgcatgcctc ccggctttga
    21961 ggcgttgcta aaaatagaga tgcccccgcc ggctggctgg cgttctccgc ggcttgccaa
    22021 gccggcggcg ggcttgctaa ttttccgagg tggcacagca gcaaacattg aaaggaacgg
    22081 cgagcttcgc cggcattggc ttgcacagtg gcaaccgggt ctcgatgacc ttcctgccgg
    22141 ctccccccaa caccggcatc cgctttcggc gcgtggacct ggagggcaaa cccgaaatcg
    22201 aggcgattgc ggaaaacgtg gttgataaca atcgctcgac tactttggcc cggggccaca
    22261 cccgcgtgca tacggtggaa cacgtgctgg cgacctttgc cggctacggc attgacaacg
    22321 ccatcgtgga actggatgcc aacgaaccgc ccattggcga tggcagtgcg cgggaatatt
    22381 gtaagttgat tcaggaagcc gggatcgtgc cccaggacga acgccgcgag tgttacaagg
    22441 tgaccgagcc catccagctg gagatggggg aaacggtgat gacgctcttc ccggacgagg
    22501 cctttcgcat ttcctgcacg agcgcggaca agcagggacg gttcacccag ttttacagcg
    22561 tggaactgac gcccacaacc tgggaacggg acctggccca tgcgcgcacg ttctgttttt
    22621 acgaggaaat cgcctacctg atcaaccatg ggctgatcaa gggcggcagc ctggaaaacg
    22681 cggtggtaat ccgggatgat gcggtgctga ccaccgaacc gttgcgctac ccggatgaat
    22741 ttgtccgcca caagatattg gatatcgtcg gcgatcttgc cttgttgggc cggccgctgc
    22801 gcgggcacct ggtcgcggtc cggccgggtc atgcggccaa ctgtgagctg ctgcggcgca
    22861 taatcgcgca aatgcgcaag ccggtggttg ccgcgcagac ctttgctcct cctccgccgg
    22921 ctccgaaaac ggcgcccccg cccgccgcgg ccgcggatga gacgcaaccg caggaaagcg
    22981 ccatagacat ccagggggtg atgaagctgc tgccgcaccg ctatccgttt ctgatggtag
    23041 atcgcgttgc gaagatcgaa ggctcccata ttatcggcat caagaatgtc tccatcaacg
    23101 aagcctactt ccagggacat ttccccggtc acccggtaat gcccggcgtg ctgcaattgg
    23161 aggccatagc ccaggtggcc ggcatcatca tgatgaaaag cgcggaaaac gcgggcaaga
    23221 tagcctattt catgtcggcc gaggatgtaa aatggcgcaa gccggtgctg ccgggcgatg
    23281 tgctggttat tgatgtggag atgactaaaa tccgcggcaa gatcggcaaa gccaagggcg
    23341 tgtgcaaagt ggcgggcgaa gtcgtcagcg aggctgcggt gactttcatg ctggccgaac
    23401 cctaggacag aattattaag cggtatgatt caccccacgg ctctcattca tccccgggcg
    23461 cagattggcc ctgattgcga gattgggccg tattgcgtta tcggtgagca tgtcgaattg
    23521 ggcgccggct gccggctcca tgcgcacgta gtggtggacg gccatacgcg gctggggcgg
    23581 cagaatgtaa tctatccctt cgccagcatc ggccttcaga cgcaggattt aaagtggaag
    23641 ggcggggtga cccgcacgga gatcggggac cataatacca tccgcgaatg cgtcaccatc
    23701 catagcgcca cgggcgatgg cgaagcgacg gtcatcggct cgcataacaa cattctggcc
    23761 agcagccatc tcggccacaa tgtggtgatc ggcaaccacg tgatcatttc gatggccgga
    23821 gtggcggggc atgtggttgt ggaagattac gcgctggtgg gcggaatgtc gggggtgcac
    23881 cagttctgcc gcatcggcaa aatggcgatt gtgggtggtt gctctcgaat cccgcaggac
    23941 gtggcgccgt tcatgctcgt tgagggcaat ccccccgaga cgcgcatgat caacaagatt
    24001 ggcctggagc gcaatggcgt gccggccgaa acccaggctg ccctcaagca ggcccacaaa
    24061 atcctgttcc gggaggggct gactacctct aatgccctgg cgcggatcga gcaggaattg
    24121 ccgcccctgc cggaggttca gtacttggtg cggttcgtgc gcgccagcga gcgcggcatc
    24181 attaaataag caccccaaaa ttgagcgctc ccgcacaaaa cgcaagcgcg gattccggct
    24241 ggccgccgct tgcgccgggc acgctttatc tcgtggccac gccgattggc aacctcgagg
    24301 atatcacgct gcgggcgttg cgcaccctca gggaatgcga cgtggtggcc gctgaggaca
    24361 cgcggcgaac cgggcggctt ctgaaacact ttggcatcgt taaaccgctg ctgagttgtt
    24421 tccggttcaa cgaagcgcag cgctgcgggg aaatcatcga acggctgcag cgcggggaaa
    24481 aggtggcgct gacgacggat gccggcagcc cgggcatcag cgatccgggg gagcgagtgg
    24541 tgcaagcggt cattgcggcg ggcctgcggg tcgagccggt gcccgggcct tgtgcgctgg
    24601 tggcggcgct gacggccagc gggctgccgg ccaccgaatt tcatttcatc ggattcctgc
    24661 cccacaagtc cggcccgcga cgccgggaat tggaacgtct tcgccacacc agtggaacct
    24721 tggtgtttta cgaatcgccc tatcgcatcg ggaaactgct ggccgagcta aacggagttt
    24781 tcccggcctg ccaggtcgtc cttgcacgtg agttgaccaa gaaatatgag gaatacctgc
    24841 gtggcacccc cgccgaattg ctggcgttgc tccggcaacg atcgcttaag ggagaattcg
    24901 tcgtactgtt gtccggcgaa gggaggcccg ccatggagcc ggatatggat tcagacttct
    24961 gcggcccggg agattgattc tttctggttg gcgggacagg cacagtttat aagccctata
    25021 tcaacaaatt atgaagaaca atacacctct gctgaactat caccctcttg gcatctatct
    25081 gcccacgcca ttcaggacct ggagcggcat cttgcgccgg ttcctagctt gggccgtgag
    25141 cattcccctg gctgggatca gcttgacggc tagcgcggcg atcattgcaa ttcaggactt
    25201 tgaaccgatc ccggcggttc ccactttttt gtataccgca accggaggct cgattttaac
    25261 aggcagttcc gggagtggcg ggggcccgtg ggggagcaat ggagaccgcc cctccttctc
    25321 tccgtattac agcggtggcg ctcaagccta tcaggtcaac agtggcacgg cgactctcac
    25381 cttcgatgct gtcaacaccg gcggcttcac tggagtccaa ctgagtctcc gggtggcagc
    25441 gttctcggct gccacctccg caaatgggct tgatttggga gactatgtgg aagtccaaat
    25501 cagccctgat aatggggcgc actactatcc cacggtgagg gtgacaggta acaacaatgc
    25561 ctactggagt tacgcgggcg gcaccggaaa tgcggccaca gcctacgacg gagatgcaac
    25621 cgcggcggta tttacccccg ctggcaaggg cgaacgcact accgatggtt acagcacgat
    25681 taccattacc tcgctgccgg aggtgagcca actgcgagtg cgcgttatta tgacaaatga
    25741 tcacgaaaac gaacgctggg tcattgatga tttgttaatc acgggaacgc tcgcggagcc
    25801 gcctcaggct ggcaacaacg gccctgtttg cgcgggcgaa acgttgtccc ttacagcctc
    25861 gaccattccc agcgccacgt acgcctggac cggtcccaat ggtttcaccg cgaatgtgca
    25921 gaatcccacg attgccaacg tgacccctgc cgcgagtggg gtttatagtg tgacggccac
    25981 tgtcaatggc cacacttccc ccgccggcac taccagtgtg atcgtgaatc ctcctcccgc
    26041 cacctcggct attagcggca gcagcgtggt tgccattggt caggcagggc agatttactc
    26101 ggtgactccg acgctcggat cgagttatgc ctggaccgtt cctggcgatg ccacgatcac
    26161 cgctggggcg ggaacggcca cgattgaagt ccgatttggc agtttcgggg gaaatgttgc
    26221 cgtaagcgaa acgaccgctg ccgggtgtgt gggggacccg atctccctcg cagtggcggt
    26281 ggggccgaac cacgcgccga ttgcgcaaaa taaaacgctg accacgacga agaacacccc
    26341 cgcgacctgt ttccatagca agcttcttgc gggcgccacg gacgcggacc atgactcgct
    26401 gagcattagt tccgccgacc cgaccagcgc acagggcgga attgtggtgc tccaggccgg
    26461 aaatataact tatgttccgc cgacggattt tattggttca gacagctact ccttcaccat
    26521 aagcgatggg caaggtggaa cggccacggc caccgtaagt gtgaccgtta cggcgaataa
    26581 tggaggggct ccgaacgtgg tcattccgcc gacgtatgat agcgcgaacc ggacttttcg
    26641 cgtcaccttt gccggcattc ctcaccgtgc ttataccgtc gaagccgcga gcgagccaaa
    26701 cgggccctgg tcctatctca agactgccac cgcggacgct tatggtttgc tcgaagtaac
    26761 cgatgtgctg ccgttatcgg tcactgcccg ttactatcgg accacttctc cttagcacaa
    26821 cagcgctagc ccgtgccgcg aatgttcgct gcggcataaa gtcggccgtc cgtcagcggg
    26881 gccaaagtct taacggtggc cgttgcgaga cagagggatg gtggcgtcgg cttaaatccg
    26941 gcgtttacct tcgcgcgatg gtttcaaccg aaaacaattc cctgtgccag cggaaggtct
    27001 ttcgaatagt tgacagtagc ggtttgccgc cgcatatagt tcttccaggc atcgctgccg
    27061 ctttcgcgcc cgccgccggt ttccttttcg ccgccgaagg cgcccccgat ttcagcgccg
    27121 ctggtgccca ggttgacatt ggcgatgccg caatcactgc cgtgggcggc aaggaactgt
    27181 tcagcttcac gcaggtcgtt ggtgaaaata gccgagctga gcccttgggg cacttcgtta
    27241 tgataggcaa acgcctcgtc cagagtccgg taggggatga gatagaggat gggggcgaag
    27301 gtctcttcat gcacgatctt catatcgtgg cgcgcggtta ccaggcacgg gcgaacgtag
    27361 cagccgccgg ggtagcgcga cccgttaagg ggttcgccgc catagagaat ttggccgcct
    27421 tcctggcgcg ccgccgcgag ggccgcctgc atatcttcga cagcgcgcgg gttgatcaac
    27481 ggccccataa gtgtgccggg tttcaaagga ttgccaatgc gaacctgctg gtacgcgccg
    27541 accaggcggc gggtcaggga ctcgcgaatg gactcgtgca ggataaggcg ccgggtagtg
    27601 gtgcatcgct ggcccgccgt gccaactgcg ccaaacaaaa tcgcccgcat ggcaaggtcc
    27661 aggtcggcgc tgggggtgac gataatagca ttattgccgc ccaactccag cagcgagcgc
    27721 cccaggcggc gggccaccaa ctcggccacg tgacggccca ttcgggtcga gccggttgcg
    27781 gagatgagcg gaacccgcgg gtcattaatc agtcgctcgc caatcgttgc cccctcccca
    27841 atgaccaggc tgaaaattgc gggatcaact ttgttggcgc ggcagatccg ctcggcaatc
    27901 ttgagggtgg caatcgccgt gagcggagtc tggctggagg gcttccaaag ggtggcatcg
    27961 ccacagacgg cggccagcgc gctgttccac gcccagacgg ccacgggaaa gttgaaggcg
    28021 gtgatgatgc caacaacccc cagcgggtgc cattgctcca tcatgcggtg gcgcggccgc
    28081 tctgaggcaa tggtcagtcc ataaagctgg cgcgcgaggc caacggcgaa gtcgcagata
    28141 tcaatcatct cctgcacttc gccttcgcct tcaggtagaa tcttgccggc ctcaagggtc
    28201 acgagccgtc ccaggtcggt tttggcctgg cgcaaagcat tgccgagttg gcgcaccagt
    28261 tcgccccgct tgggagcggg cacgagccgc cagtcctgga aagcctgttg ggcgcgccgg
    28321 gccacccgct cgtactcctg cggcgtggcg gtgcggacgg tggcgagagt gcggccatct
    28381 atgggcgaga tgcttttgag cagcttgccg ctgccgaacc attcaccgcc aaaggcgccg
    28441 ggattctcgg gtttgaggcc gagtcgtttg aataaggcgg aggtcaggga gtatgtcggt
    28501 ttcataaaaa agccggcggg cctggtggta ggcgcgggtt aaagcaggcg gcattgctgg
    28561 tccataatgc gcaaagccgc ttcaatgatg tcgtccttta cgtccagtac gggccgcaag
    28621 cgaatggagc gctcgccgca gcgcactacg agcaggccca gttcgtaagc gcctttccag
    28681 aaagcgtccc gcgtcgcacc gttgggcaga tcaaacgcca gcatcaaccc gcgcccacgg
    28741 actgcggaga tcaccgggta tctccgagcc agcgcctgga gttcagcgag gaaacgctcc
    28801 ccctggcatc gcgcgttctc gaccaatcgc tccgcttcga tgatccgcag gtaatgcgtg
    28861 gagcgtacgt aatctgtaaa gttgcctccc caggtcgagt tgatgcggct gggaagccgg
    28921 aagcagttat ccttgacctc atcaagccgc gggccagcca tcacgccgca gacctgcacc
    28981 tttttgccga aaacgagcaa atcagggcgc acgtcaaaat gttcgcagca ccacattttg
    29041 cccgtaattc ccagtccgga ctggacttca tcaaagatga gaagaacgtc attctcatca
    29101 caaatccggc gtagcgcgcg cagaaactcg ccccggaaat gattgtcgcc gccctcgccc
    29161 tggatgggtt cgataataat ggcggcaatc tccggagcct tccgggccag tacgtcccgg
    29221 atttgctcca gcgcctgcct ctccttttcg accaccgccg cctcgcgctg cgcgggcggc
    29281 agggagaaat ccaggtttgg cgcgattatg cgtggccagg gaaacttggc gaaataggca
    29341 atcttgcgcg gatccgtatt ggtcaggctc atcgtgtagc cgctccgccc gtgaaacgcg
    29401 cgctcaaagt ggataatctc cgtcccgcgc tcgcctagcc ccgcggcgag gttccgccgc
    29461 actttccaat ccatcgccgc cttaagcgca ttctccaccg ccagggctcc gccctcgata
    29521 aagaaatagc gctccagcgg caccaggctg gccacgcgcg cgaacgtttg aatgaacgcc
    29581 gcatactgca cggtgtaaac atcggcgttg gccaccttga cccgggccgc cgccagcaag
    29641 tcggccttca cttccggttg atcaaaatag ggatgattga agcctatggg ttgcgaggcg
    29701 taaaagctgt agagatcaat caattccctc ccggtcaccg catccacaaa ccgcgagccg
    29761 cggctgcgcg ccaggtcgaa caccagcttg aacccatcca cgaggatgtg ctcctccaga
    29821 cgggcaatga cctgttgtgg cgaaacggca cccgaagccc ttggtcccgc cgtcggattt
    29881 gcggcaatgc tgctggcttg ttcaatgaca ctcatactgc tattgatttt ccacatctgg
    29941 aaaacccgag acagactagt gaatagttgc ccctcaatca agtaaaatag cctctccgcg
    30001 aacctcattt tcaggcaaaa tcctacaccc agccattcgt cccgagcttt tttgccgcgc
    30061 aaagtgaggg gcttgatagc cagttcccct gcgccacgac ggtgcgggca aaactttgcc
    30121 ccgcgcgccc gggcccgttt tgacgagcaa aggtgaatgt tggcggttaa tcaaagccca
    30181 gaaaaggtct gcctctgtac gacaggcgac gaattccgcg ccgtggttct tgaagccttg
    30241 accagaagga aggagaagcc acccaaaccg cgaaacagtg cgaatcaaag cttgcacgcg
    30301 ttccagaatg gtctaatttg ccttggaaac cagcggcggg gtagccaagt ggtaaggcag
    30361 ggctctgcaa aagcctcatg cgtcggttcg attccgaccc tcgcctccaa ttccaagttg
    30421 ccatgaggca acggattgca gaagccagag gaggaagtgc cagtgaaagt gccagtgaaa
    30481 ttattaccgg gacacaacca ttgaactacc ccggcaatac ccggcaatca ctcgcaacga
    30541 cgggattcgc tgcccgtctg gtgtagctga tgcacccaaa accacgttca gcctcatagc
    30601 ctcggacgaa gaaagtctga gctatgaaac gtcgtttcat cctctaccgc cgcaagcgcg
    30661 gcggcatgtt ctacgttgaa gacaccgaaa cccggaagca ggagagtctc ggcacgcgcg
    30721 atcgcgcaga ggccatgtct ctgctcaacg cccgcaacga gtcggttcgc cagccgcaac
    30781 tgaatcttca catcgccaag gcatatctgg ccgggacaga ttcaggcgtg tcaacgcgaa
    30841 cgtggcaaaa tgccctggac gccatcatcg aatccaaaac cgggtcaacc aaagaccgct
    30901 ggcagcgcgc cgcgaaagaa aaggcaatgg acctgattcg tgaccgggtg atcatcgaga
    30961 cacaaggcga gcaactgctc gcctgtctca aagccggaac cgtcagcacg aatgtccatc
    31021 tacgcaagct gcacaacttc tgcctcgcga tgaattggct gccctggccg atcattccca
    31081 agcgcctctg gccagaagtt cgttttcagc ccaaacgagc gatcaccatc gaggagcatc
    31141 agctcatcct ggagcgcgaa aagaatccgg agaggcggag cttttacgag ctgtgctggc
    31201 atctcggcgg ctcgcaaact gacatcgcca acctgaaagc ggaggatatt gattggggca
    31261 accgaaccat cgcctacaca cgccagaaga ccggcagtct cgcgatgatc cactttggcg
    31321 aagagatcga ggcggttctt cgccgtctgc cagacaccgg cccgttgttt ccctacctgc
    31381 aatccgtccg gtgtggcgac cgtgcgactg agttcaagca acgttgccgg gggcttggta
    31441 tcaagggtgt gtcgctgcat tcctaccgtt atgcctgggc cgaacgcgcc aagcaatgcg
    31501 gctacccgga gcggtttgca caagaggcgc tgggccacaa cagcaaggcg gtgcatcgcg
    31561 cctatgcgcg aagggcacaa gtgcggctgc catctttgga aagttatgaa cggcaggcaa
    31621 cggatggccg aatcattccc ttgccgtcgg tttcgtcgaa gctggatcgg aacgaccact
    31681 cgatctcagc ggccggttga acgccgcact ctgcgtcgct gcgcgcccgc gagccttctg
    31741 cttgcgggcg tttcgttccc gccagtagct gcagatcgca tcactggcac gggcgagcca
    31801 cttttcgtcc cggcggagtt gctccagtaa ttccgtggcg aagaacttcg tgctgttgcg
    31861 tgggggatgc ccaagcggtt tcaacaagcc tgcggcgaca agcatgggaa cttcatgcgg
    31921 ttcgaagccg agaaaccaag ctgtttgggt ggcggtcagg cgggcggggg cgattttcca
    31981 actgaggaac tcggtttgtt gcgcggtctc gttcatacaa acgacaccct tatcaagacc
    32041 gaatcacttt gtggtgtatc caatacacga gaactacacc tgaattaccc actcaagact
    32101 ccgcctataa gcctcttgcg cctttggcct gtttggtcat tcctaaacgt gaatcgcagt
    32161 cctccgggcg tggcggacgg gtttcgtcgg agtctgctgc tctgaaatct tgtttgtgcc
    32221 ttcgtcgagc gaatcggcgt ccacttggct gggggcctgg ttcttcgtga ggtcgagctt
    32281 ttcctcaatt tcgctctggc ggcggcacaa ctcctggtag cactcctcat gctcaaaggg
    32341 agcgccgact ttggtttcga gttccttgcc ccgcttgtgg gcatcggcaa tatccgtttc
    32401 cagcttgcca gcgcgctcct cgaaaccttg aacggtcgct tccaatgagc ggattgtgcc
    32461 cagcgcagtg tccgtgaccc gggcgccgta gctgttgtgc ccgcgcaaca ccacctcgac
    32521 cgcgttgttg aagccgggcc gcaggaacaa atcgaaccca gcaaaacgac cgatgcgcac
    32581 atcgtcaccg aaacggtttt ttacttttcc gcccggcgga caatcaattc acccgcgatg
    32641 ccccggttgt cgagggtctg gccgtcgagt tcgatggtga atttttcgcc agaagtatcc
    32701 tgacgcacgg cgatgtcctg gcggagattg gcaagccgcg cggtgaaggt ttcggcgtcc
    32761 tcgtgagcac ggcgcagatt cgaccggatg cggtattgct cctcggcgtg ggcggagcgc
    32821 agccgtgtga gccgcatcaa ctccgcatct acctgagctt tctcgatcac cagtggattg
    32881 ccggaagcga tggctttcac ctcggcgtag gtaagtgccg ccgattccaa gtcttccaca
    32941 cggcgaatgg tcatgtcgcc gctcatcact tgggcgatga acttggcttt ggtctccagc
    33001 gtctgccaca tgtaggcgtc gaacgagcct tcggtgacgt agcgaaaaac cgagacgacc
    33061 gcgttcttgt tgccctgccg cagaatccgc ccctcacgct gctctacgtc ggcgggtcgc
    33121 cagggcgcgt caaggtgatg caaagcgatc aaacgctcct gcacgttcgt ccctgacccc
    33181 atcttctgcg tgctgccgaa cagaatgcgg accttgccgg agcgcacttc ccgaaagaga
    33241 gcgagttttg aggcgtcgga atcgtagtcc tgaataaacg caatctcccg cgtcggcacg
    33301 ccgagccgct ccagcttgtc ggccatatcg cggtaaaccg agaagccttt gtccttcggc
    33361 gtcgagaggt cgcagaacac aagctgcgcg caacgttccg ctgcggtggc ctgccagatg
    33421 cgatggatgt tttcgacggc cagattgacc ttgccttgcg gttcgtcggg cgtcgcgggt
    33481 ttcatcaggc ggagatccag cgccgccatt cgtccctcag acgtgatctt cagcatgttg
    33541 tcttcgctgg gatcaacccg gccggttttc aaccggtcag cccgggcagc aagttcctgg
    33601 acaaatgcct tcaactccgg cgtcgcgggc gcgttgcgaa tggcaggctt ctcaccttca
    33661 agcagcgggc gaggcagatt gagcatcgct gccgtctgca cgtcggcact ctggcgaaac
    33721 atctgcatca actccggcac gtttatgaac cgggcgaacc gggtgttgag ccggtagccc
    33781 gcgccatcag gcgaaagctc cattgccgtc accggctcgc caaacgtcgc tgcccacgag
    33841 tcgaaatgat gcaggttatg tttcttgagt tggtcggctt ggagataccg ctgcatggtg
    33901 aacatctccg ccatgctgtt ggcgatgggc gtgcccgtgg cgaacacgac cccaccccca
    33961 ccattgagcg actggacatg gcgcactttc agatacatat cgaaagcgcg ttcgctggcg
    34021 gtctgcggca ggccggcgat ccgcgtcatc ttggtgacgt aaaacagatt cttgaaatag
    34081 tgcgcctcgt ccacaaatag ccggtccacg ccaagttcct caaaggtcag cgtgttgtcc
    34141 tttttgtgct cggcggcaag agcctgcagc cgggcttcca accgtttctt cgcccgttca
    34201 atctccttca ccagacggcg gttggatgaa tcggcgtgtt ggcgcttgat catctccaac
    34261 tcgtggagct gttcctgaaa gaaccgctcc tgtgtttcgc gggccagcgg gatgcgctcg
    34321 aaaccggagt gcgtgacgat gacggcgtcc cagtttcccg tcgcaatccg gctaaaaagc
    34381 ttcttccggt tctgggactc gaaatcttcc ttgcccgcaa ccaggatgtt cgcgccggga
    34441 tacagcgtca gcagctccgt cgagaactgg ccaagcatgt ggttggggac ggtgaacatt
    34501 ggtttgcggg caaggccgag ccgtttgagt tccatcgcgg cagcgaccat ggtgtaggtc
    34561 tttcccgcgc cgacgacgtg tgccaggagc gtgttgggcg tttggagaat gcgccacacg
    34621 cccgctttct ggtgtctgtg gagttggatt accccgcttg cgcctgggag cgtcaggtgg
    34681 tcaccgttga atgtgcggac gcgcgtgtgg ttgaacatgt cgttgtaaag ccgacagagc
    34741 cgttcccgcc gcgaatcgtc cgcccacacc cattctttga agcgttcctt gatgcgctcc
    34801 tgcttctcgc gggcagcctc ggtggcctcg gcgttgacga caggtttctt gtcctcattg
    34861 aggtcgtaga ccgtaggcgt tttgaggttc agggcatctt caatgagttc aagcgccgtg
    34921 taacggttcg tgccccaatc ggtcgtgttg gcggtcgctc ccctggcttc ccaattcccc
    34981 gtgagatgcc aagtgccgag tgcatggata tggccgatct ccacgccgga ggggatgttg
    35041 agcaactctt gggtgaactg cttcacgtcc tcgggtggca accaggaagc gcccaaccgc
    35101 acgtcaatct ccgtggcatt caaatcagcc ggctgcaccg acttcaaagc ctcgacgttt
    35161 tcgaggaagc gcgagtccgt caccgcggca gcgtccgcaa cgaccagttt ctcgcggaca
    35221 ttgccggaga ggtagtggtc gtcggtttcc cactggttcg tttgcggatt caggaagatc
    35281 atgcctttca agtcgggcag gaattcctcc gtcggcttat tgagcagcgc cgccatgtgg
    35341 tcgaggtcca cgcggccacg ctcgttcaag gacacgagca acgcctcctt gggggaactg
    35401 acggcttcaa ttggctgccg gtgatggatc gttcgctcgg tgaagatggt ggctttggtc
    35461 gctcgcttgg tctcatcgtt gtagtgctcg agtgaaagca gcaagggcag gtcggggtcg
    35521 ccgtcgaagg cgcgctgatt ggcgcggaga ttgatcgggc gaaaacgtga ccaaaacaag
    35581 ccttgtttcc acctacgatc tggcgtaaaa ctccgaaagt tctttctcaa agccggaagc
    35641 ggtcgtttgc ttctggcgta gagtctttgc gttgcgagga ctcgaatctc gttctaaaca
    35701 cagcgacaaa tttggaaacc aagagcagca attcacacct cgacgcgcgc gagtcgcgcg
    35761 tcccggtgcg tttgctgctg ggccgcgtcg ctgtggcctt gaacgggagc gtgctgggcg
    35821 cgcattccgt ttcctggcga ggaaggaggt tcgagtccgg gcaaaaaaga cccggaggaa
    35881 accaaaccag aaacagaaac tgaagaacat gaaagcgatc aaacacatca ccggcaggct
    35941 gctcacgggt tgtatggtcg tgttcggcct ggttatcaat cttggcgcac agaacaaacc
    36001 gatcgacaag ccacccattc cgggcacgta ctactcggca aaggacttcg gttttggacc
    36061 gccttaccca ttcaacccac atccagaact ggaaacggtc gaagtcgagc cgggggtttt
    36121 cgtcatagac gacacgctga ttcccgacac gccggagcaa gcggcagcaa gagcagcgca
    36181 ccaagcagcc gttgaacaag cgaaagcaat tgcctccgat ccgatccttg ctgaagctgc
    36241 aaggcaagcc gcggaggagg cggtgcggaa gcgagagcag gcgtggcagg agagattgca
    36301 ggcggttcag ccgtttttgc gctcctgggg ttggcccggt gacgccgaaa cacaccagag
    36361 acgcgaagag gcaaaggcag agttggcaga gttgaaacgt aaggcggagg cggcacgggc
    36421 aaacgcacca caggatgaag ctgcgcttga tgagattgcg tcccgcctga atgtcgagcg
    36481 cgttcgcgta cgcactgacg gcggcaaaga agtccttggc ggcgcagtcg gcgaccagcc
    36541 tgtttattat tcgagccaga acttatttgc tggtgcaagc atcagcgccg acgagttgtg
    36601 gccgaccaac accgcccctt gggcgcatgc ctcgacagga cggaatttga ccggtacgaa
    36661 cgtcaccttg ggtttgtggg agattgacgg cggtgttctc acgaatcatg cagaatttga
    36721 cgctcgcgtg cgccaggtgg acaatacggc gacgaatcct gtttccagtg atgctcacgc
    36781 aacgggcgtt gctgggacaa tgggcgcggg cggaattctc cagtttacgc ttaatggcca
    36841 gcctgcccgt ctgctgcggg gcgtggcttt tgagtcaaag ctcaacgctt acacttttgc
    36901 gaacttcaaa caagaacggc tcgaggcggc tgcctgttct gtgacgggcc agccgctgcg
    36961 actttcaaac cactcttacg gtgcttctgg cggttggata tacacaacga tattggttca
    37021 ccagggtaat caaacgaacc tcgtcacgac ttggctttgg cgcggtgacc cgtcgctgac
    37081 ggaagaatgg aaatttggct tctacactcc gggtgctccc gatattccag atggctcagg
    37141 ctgtgtggac acggatgctt ttcttgcaac caacgcgacc cgtcacttga tggtttacgc
    37201 tgcagggaat gaccgtctga ccgggccggg cgggccgacg aattactatt atttctccgg
    37261 gccagggccg tatgactaca cgcaggtcac caatccttcc gcgaattcac gcgattggcg
    37321 taatggcgat ggtgataccg gcgggtatga taccgtgctt ttccccggca cggcaaagaa
    37381 tgttctgact gtcggggcgg ttcgagatgt gtatactttt tctggcacga atctcatcat
    37441 gggtttcgcg accaacgctt ccatcgtgct tgcgccgttc agcggatgcg gaccaaccga
    37501 tgatggccgg atcaagcctg aggtggtggc cgtaggccaa agcaactccc tgcttcgaac
    37561 tttcggaatc gcgacacccg ctaacaacgc cacgaacgga tattttatcg cgattgcagg
    37621 cacaagcttc gccgcccccg cagtggctgc cgggcttggt ctggccgcgc agcgccgttt
    37681 gcagcttttc ccaaacctcg acccggactt ggacgcatgg cgcggttcga cttggcgtgc
    37741 attggcgatt cacactgcgg atgacgtgga cggtgctggg cctgattttc aaatgggcta
    37801 tggattgttc aacgtggcct catgcgtagc acaggtggaa cgcgaccaac agcacggacg
    37861 cggaacgcat ataaaggagt tcgaaatcgg cgtgagcaac actgtgagtt gggaagtgga
    37921 cgtcgatagt gtaccattca aggcgactgc tgcttggagc gacccggctg gcccggcgac
    37981 taacgttgtc attgttgacc tcacaaagcc gatgctggtc aacaacattg atctccgcgt
    38041 ggaaaaggtc ggcgcgacca atgtgtacta tccgtggatt ctgaatcccg atctcacgaa
    38101 caaaacggaa tcggcccgcg ccgcagccgc cacgactggc gtggacaacc gcaacaacgt
    38161 tgaacaagtc tatatcgcca acccgactcc gggaaagtat cgcatagtcg tgacgcattc
    38221 cggtggtctg ctgggtggtc cagttccgtc ggcacagtgg gtttcagtgc tcacgactgg
    38281 cgaaacgcct gtctcacctg ttttcacttc gattgaggcg agtccgtccg ggacaaacgt
    38341 gctcctgact ttcacggcag atcccggcgc ttattttcac ctgctcagtt caaccgactt
    38401 ggtggcgtgg cagaccaacg cgacggtcag ggcggaaggc gtgacgaatt cggtcttggt
    38461 aagctcaaca cagccgtacg agtttttccg tctccggcgg cagcagtaat cgaacatgtc
    38521 gcggcgattc ctaaatatcg tggtgattgc ggcagcttgg ctgggtagcc cccagccaag
    38581 ctgcgcacag ggaacaatcg tcttcaatga acctgctaca ccaattcctc tctttacgac
    38641 aggcttcgct caatactact cgtttgattt ggacggagat tcagcaaccg atttcacatt
    38701 cacttacagc ttccagtttc tcggcgtcca accggaaggc actggcagaa ttcttacctg
    38761 gcttgaccca ccgccaaacg ttggaggcag cgtcgctccg ctgccactag gtttcgtgat
    38821 tggcgcaggt agcgaaacag gcaacttggc gtggtatgga catgcactcg actacaacac
    38881 cctcgcgatt tgtttcgatg tcggctgtgc gggggcattt gtcggccagc acgcatacat
    38941 gggcgtggaa ttccaacatg ccggagctac gcattacggc tgggttttgt tgaacgtcgc
    39001 acaggcctac ccgtctggcg ttatcgaatc ttgggcgtgg gacacgcgcc caagcgcgtc
    39061 catctttgca ggcgcagtgc ctgagccgtc aacttggact ttgtttggca ttggcgcatt
    39121 ggcgattgcg ctgcttcgtc gacggtgctg aaaacgcggg gacttcttcc cgccgaaacg
    39181 cgcggtgacg tgaggcgtgg gcacggggtc gctcaggcgg cgacggcctc acctctggtt
    39241 tgctgctggc cctgcgaacg cgcaaccgcg cgccgtgcgg agcgggcgat ctgggcgatg
    39301 acgctttcct tctggtcgtc cggcaggtct ttcaccagcg tatcataggc gtttttaagc
    39361 gcggggttcg tgttgatgcg tttcatcaag ccttcgcgca tccgctgctg gcgatccaat
    39421 tcgcgcacct cgttcaggac gacggtgcgc tccagccgct cgcggggcat cgcttggacg
    39481 tagttccaat acttggggtt gtctttgatg taggcatcaa tcttcgcatc cacttccggg
    39541 ttggtccgga acgtgggctg atcggtgggg gcggcattgc cgccggcgct cgatggcgct
    39601 gaggttttcg gtttgctcat aacgattcgt ctttctggtt cttgttgcgc ggcatttgcc
    39661 gtgttcgccg aa
//
LOCUS       DAOPQG010000020        39307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1367_length_39307_cov_15.438576, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000020 DAOPQG010000000
VERSION     DAOPQG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 39307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39307
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_39307_cov_15.438576"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(534..3785)
                     /locus_tag="PKI07_03720"
     CDS             complement(534..3785)
                     /locus_tag="PKI07_03720"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC50190.1"
                     /translation="MKPFRIFRGFAFTGPGISISLLLLLLFATGCSKEARKNRYLSRA
                     NADFAASRYEQAEIHYRKALQIAPENSTAIGYLGIICQEQGRLPQALAYLRKATELAP
                     DDSEFQLKLCLTKLALGARQEARADAERVLQKQPGQADALVALAETTTSLKEAQETAR
                     QIKALRQQDKDRPGYHLALATLYLRQGQIAEVAPELNQALALDPKFPATHTALGTLYW
                     LSNDLARADAAFKTGAELSPPRSPRRLKYAEFKLKTGATNEAVKILEELTHKAPDYLP
                     PWIYLAQFAFTQNRLEECSAFLQKVIVRDPYNIQAILLGANVKLQKGEVSQATEELLR
                     LSKMFSHSPGIQQLLARCYLLNGEDAKAEASLKDALALDPNFPEAVLALAELSMKKGD
                     FAAAARSLEQLLKHDPELPQVHMLLAAAYAGQKKFDPALAVYRQMQARFPRNPQVPFL
                     MAGILVQQKQMSDARKACNQALELAPGFLPPLELLVDLDLDDKQPQAALKRVQEQIER
                     RPKVPELQLLLAKIYLQQEDLAQAETTLLKIIELAPQLQTPYMLLARIYLSSGKTQQA
                     LDKLNSYVARTNDAPTQLQIGIIQDSLKNYPAAREAYEKALAANPKFGPALNNLAYLY
                     CENLDQVDKARGLAERARQLYPADPSIADTLGWVFYRKGDFPKALTLLAEAATAAPNE
                     PEIQYHLGMVRYALGQEGAARSALEMAVKSDKNFSGKEKARQNLAILAIDPKASDPAA
                     QTQLEKRLAEDPNDSIALVRLAGIHIRNGAPEKALKTYEQALAVNPKNAFVLASLARL
                     LAAPGSLKNSEKALGYAKDAHAAAPEDAGISQLLGHLACEAGDYKWAASLLKDAALKL
                     PDQPALFYDLARASYGLGQLSAAETALQNALKGTATFGQADEAKRFRSLLQAYRDPAQ
                     RATVAAQLPEVLKADPSYIPALMVNQLLLQGKGDFQAARKTLDHVLAINPLFVPAARD
                     LAILCFDHFPDEPKASELAMKAREAFPDDPNLARVLGILAYRREDYSNAARFLKQSLQ
                     ARSNDAELFYYLGMAQYRLKSSAESKQSLNRALNLNLAPQLAAEAKRVLGELK"
     gene            complement(3895..4833)
                     /gene="xrtC"
                     /locus_tag="PKI07_03725"
     CDS             complement(3895..4833)
                     /gene="xrtC"
                     /locus_tag="PKI07_03725"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPDSG-CTERM-specific exosortase XrtC"
                     /protein_id="HOC50191.1"
                     /translation="MKPHPEAHKPPVAAQAAMLSPLLRRQFRQLAICTAVLAVCFARP
                     LFELARFTLHSELYSHILLIPVVSLYLAWLNRHKLPLASEPDRRLAVLPLGIGLLLLA
                     GYYFAPHWGLKLARVDSLAWTTLSFVFLFLAAVLFCLGKSTLRVLAFPLAFLIFCVPF
                     PVLVKDAIETFLQYRSADAAELLFGLVGTPLLRNDVDFQLPGFRLQVAPECSGIRSTL
                     VLFISSLVAGYLFLRTPWKRALFAFLVIPLGIFRNALRIVTIGELCIHIDPGFIDSAI
                     HRRGGPVFFVISLAPFLLLLYYLHKTDVRKPQANDV"
     gene            complement(4954..5745)
                     /locus_tag="PKI07_03730"
     CDS             complement(4954..5745)
                     /locus_tag="PKI07_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50192.1"
                     /translation="MSSSEPLIETSRQTDQQTLSTRLSADYQINSKTSLELGLGQNLR
                     LIERSSKGAALNNFNSWSASSWLNYKVSPGIKAAVGAGFEYIDCTSSSDITSEQIQGR
                     ITWAVSKKLEFVASGGVNNMQFLSSGASDLASPIFSLSVQYAPYETTTFFITAYQAVQ
                     PSYYQDQVTENTFVSAGLHQRLLGKLYLDLSGGYGNSAYHASAATPVASHRDYETTSF
                     SASLSTPFLKRGFAAVFYSVNRNASGAADYDYTTTSVGLQLSCRY"
     gene            complement(6880..8058)
                     /locus_tag="PKI07_03735"
     CDS             complement(6880..8058)
                     /locus_tag="PKI07_03735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOC50193.1"
                     /translation="MSSLLIAADICPIGNNRPLFMAGDARGLLNDLLPEFERADLMVA
                     NLECPLIGEPSPIMKTGPIFGAPGDCIKAIKNAGIGVLCLANNHILDHGGAGLINTLK
                     VCAQEGVETVGAGKNLAAAGRIVVRDCGGVRVGLLAAAEHEFSIAGENSPGANPLDLI
                     TFVRNIREHRASFDFLVVLLHGAAEFFAPTPRIQNTCRFMIEMGADVVIVQHPHILGG
                     YENYLGGHIVYGQGALLMDEDIYRNRKSFHEGFLVKLTLNVQLTTAGFQKSQNSPSSQ
                     LTLIPFTQSDPEPGARRMVPEREQAFYECLAERSRMILDKRFVEAEWVKFCEENKHGY
                     IGSLLGFNRVMHKLNRNGLLFRLLYGKRPLLGVRNLIQCETHREAIETIFQHQLCERS
                     "
     gene            complement(8162..9640)
                     /locus_tag="PKI07_03740"
     CDS             complement(8162..9640)
                     /locus_tag="PKI07_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50194.1"
                     /translation="MALTGIAQDASLRSSVSQYGITWTFAEPGRVGQFVNGDWYVVAP
                     VVVAAIEPAPLWGKAVGELIDRGQVRESNYPGKQARNGSVLNPPARKREGGYDSRIPS
                     GRYDPTLFAHLPIRMKPGDSLVSTISRRNDEITKFGGQHVDPLRVAAVLSCVAEPPPA
                     DAFRPSYCDSANSPPFRARDLRRELLLKVPRPPSAPPLLDSYIKAFQKPWLDTVEFGF
                     AAPVENLPHYGQHMAQAVGEATLLLLMDYPGPEKERLLVNLVQVGIDLWGAARAGMSW
                     PAHGGLHSGRKWPIIFSGLMLGDSAMQSPTKNHPELHFGEDDQTAFCPYEYRGKTHAA
                     GWTGARAIFTGHSLAGTGGDRGKWESGWGAVDLFPPTEWPKKNPNDLPASEDYRRANT
                     SGAWVAEALAARMMHAEPVWNHDAFFAYVDRWMTEDDTSFVKQIKEAGFKDLTRVAFG
                     NFQRQGYVWGPKFVREMWDAHRNHLGADAHGKNTPASTTTWR"
     gene            complement(9761..11140)
                     /locus_tag="PKI07_03745"
     CDS             complement(9761..11140)
                     /locus_tag="PKI07_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOC50195.1"
                     /translation="MVVQAAEAKAIRATPNAGAGLPVRRTVYLMGASLGTGNRGVSAL
                     GASLVKLVRLSAPVAEPALLIGSRTNQPFDLFLGDRKLRIPIANFRLSPRARMSEQLV
                     WWFLFSVLYRVVPVGWVRNFLSRKHPLIHASTEAVMVGDIRGGDSFSDIYGLKNFLQG
                     SLEVLPVILVRGTIALFPQTYGPYKTSLARTLARFILRRASVILSRDCESLATVKDLI
                     GETSRCRFCPDVAFALEARPPKQFLTIPPLNLSTSDASPSTTLVGLNVNGLMFNGGYT
                     RKNMFGLKLDYAEFLAQLMQALLGDPAVRVLLVPHTFAPAHSVESDPEACRKVRGRLS
                     PELQPRVHLVEGEYDQSEIKGVIGQCGFFVGSRMHSCIAALSQGIPAVGVAYSKKFAG
                     VFDSVGAAGWVVDGRELTAEQGVAKAMEIYRRREALREPLAEKVREAQATLYRTFQEL
                     FSSRDESLN"
     gene            complement(11258..14368)
                     /locus_tag="PKI07_03750"
     CDS             complement(11258..14368)
                     /locus_tag="PKI07_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50196.1"
                     /translation="MKFERILLFVWLAFSASGAIRNVYFDSQAAGSGDGSLDNPFRTL
                     SAVSFPAIQAWVNAGDDVHLQLKRGSVFRENFTFSASGTAAHPIRLQDYGAGAKPIIS
                     GEAVFTGWSLAGFGGGATWRLAAPNRVFYLWLENQYLTKGTSAGTLSDGAWVWESGQL
                     YLRYDAGNPDTLTLSVTGAVRPNALRLNTVGSLIVSNLIFTKTSSYAASVTDANDIQF
                     DDTEWLECGSQLNLNRSVNIAFRACRFEGSYGNQMALIEGPQTSASFVFCLFANQFVP
                     AALQILSAASVLVANCNFVSVSGTSVSSAATGAVSLANCILTGHAIQTNTSGSDPIKS
                     TGSPILVQNSLILPNGYNPSKHMVNVTEIGRNLYQDPRYVSVNRESILSLATDDYENL
                     DDWIVFANLAKAKGWRAVLALSKTDTLKPSDKTKMQAMIDQGHEIASHSRSHAFLTTA
                     LGCYLKYVGTGSNCLLTIQGNRLKTIVSGGNGGENLDINLLQGNVDTTSELVSTLDAP
                     PAYTCRLATIYSHAVPSRLLETVTDLDIALAEQLLPYNVPAFWTNEIWLSKEDIQSAF
                     TAENGGPYSCRSFFYPGGAFDDAVVSQVIAAGYLGARADETSPVFSHMENMDLYHITV
                     PQGTTYGYVLMLRFERNTEDTSPQSNGFTGANLAYSTNAYRHSRAGDFNGTNTCVFRD
                     ANADFNFVRRDWHFSGRFWLRGQTGAGTLFSFTNAATAARIEVNDQLAVNFVVRSNAA
                     EIFRLSTPPAAVSPGQYQKISVQQLFDRWFIRVNDVVVATTTNTLRLPAATGQVYIGC
                     AANPDDGQLGNFYNGLMDDVVVSNGTYYKTQGLADMLCQFGGMMCTVSHNESGIPREI
                     LRIFLDALADYSGNLKVMTLSDALAYLRSRGVLSADGRSLKRYDLGHHADYHLRADSP
                     CVCAGDPAVLQGIPSLHDLDGTPITDANGNLLLPGSGIDMGAFVNRNIGYSAPRLTMA
                     PLKGGQSPKLLLVGQLGIRYQIDFSPDLSLWNPLTNLLCTDNPMVIEDPTNTPIRFYR
                     AVVSGN"
     gene            complement(15395..17137)
                     /locus_tag="PKI07_03755"
     CDS             complement(15395..17137)
                     /locus_tag="PKI07_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOC50197.1"
                     /translation="MLTRLILATALVVVSAPQGPAATFYVSTNGGDNSPGNEAQPWRT
                     IQKAANTMSAGDTVYVQSGHFDERVTTVRGGTAPENRITFRAIGTVTMKGFAVQHPFI
                     TVDGFDITGATSASNSDAYVAVSKTGDWFELLNCTVRDGIQAVRNDMVFEPPNKISSS
                     TGGFLAAGFVPGMTVQFYRATNVALANGEKSFLVSSVTDTSLVFATSAIAADGPKPTY
                     LTGSAIYGLYLADGTDNCLFRSNRFSNLSYRYWFVQGSNHLFEANILEHNNGWDLIFF
                     MGADHKFVRNLFQNNGWGTFIPSPDVFDCWPVKYERITFSNNFVINMMGVINAQKYNK
                     TVSGPFWIKNNVFVDVGWLSIRMPNTTFENNTLLRVARQANALVQVEKHPLIFDLGDY
                     ATNAVIRNNIFVDCGQATGPTTPDQVGWYRFLGPTNTVTLENNFVAGSAPNYDAKTGF
                     PEPDPTLNGGDPGFVNLDDPLGPDGVPFTADDGLRLRADSKLVGKGAGLVDLGAYNTR
                     PPLPRLSIALSPPTNIQLSWPTSANTFELQSALTLTSPWSAVEVAPTIEGRSFVATFG
                     HTNPATFFRLRQVP"
     gene            complement(17207..18454)
                     /locus_tag="PKI07_03760"
     CDS             complement(17207..18454)
                     /locus_tag="PKI07_03760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC50198.1"
                     /translation="MNTVLLNDRSIEACAEGSALSWEIFCSFEDVRHLQPQWDDSAAR
                     LGGSIYMTFDWCRIWWQFYGERKALRLFVWRVNERIVGLLPIYIETVRLGLLRLKVAR
                     LVGANIPPKVFDPPLDPAFTETIFEQLFKRLLAEDGCDLVSLGPISERLNLAEPLQRA
                     CKLRHDLTRNLESLRGVHSVFELPANMEAYYGALSKNERKNRRKYELRLLKKEYETRV
                     EVITELSKAESEFECFAQQHAAQWAAEGKTGHFGAWPKALEFNRALVRAQAALNRVRF
                     VRIVANEEVIANQYTYAFGDRYYWELPARAIAPKWDRFSLGPTGLVTLIERGIEEGKR
                     YLEGGLAHYDYKIRLGAREYGTFTFRVRRCGAQVGLKLAIFILFRKAMEFGYHKLWYR
                     RIAPRLPLAFWRPQWRLWLQFDF"
     gene            complement(18540..19973)
                     /locus_tag="PKI07_03765"
     CDS             complement(18540..19973)
                     /locus_tag="PKI07_03765"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOC50199.1"
                     /translation="MKRALFPLVFGGLMLWGNPLVEAATYYVALSGNDANSGAQTAPW
                     RTIQKAADVAGAGDTVLIGPGNYGECVKICHKVSAGACVTFDGQGVATNAQFQIASPN
                     VRIRNFILTGLTTKFTGAIDLRRGAHGAYIEGNQIDTAYADSVRAVNWDFGSQTPIDP
                     NVASRVTITNNSIAHIYNTVCIILMGTNNLVVNNRMRDIRDADYFHVFGVSNTVRGNI
                     CIGFEDTPNSGNHMDCFQTFGLNGHASKHMIIEENVFGYNYNPTDVDIAQICQLEQNL
                     NHEIHNWDIRNNIFFGLLKGECGIPSVRWHNNVFYQIWTNASGGAIIINWAESGRNNA
                     TNAQILNNVFLGCGKNDQSGWYSVGDGSSIVTNTWKVDFNYVAALDGSPKRAGLPQTN
                     QRWYEPHGINGGSDCFQDIRQHDFRLRQDAPLRNKGVNLSNYFSRDHTGNARPVSGNW
                     DIGAMQFVASENRVVLPPSFKGIVVHP"
     gene            complement(20150..21553)
                     /locus_tag="PKI07_03770"
     CDS             complement(20150..21553)
                     /locus_tag="PKI07_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC50200.1"
                     /translation="MLLAAVVWLQLQRDFIRGLSCAMFIWVSMTTFLRIDLPGSLPEL
                     TIHRLLLILVLIAWIRKGGMAKLASVPLLGYFAFWAMANLVSLAGTQIDFVISLKRVL
                     DFVLEVFAAYIITATSLKTREDVLRVLRAAWLGLLVVAVFAVIERYTGFNPVDRYIPG
                     YAREDVQFRDILSTFQHRILLGTALAMGVPLSFLPLRSRKRVDEGGAVFWGTLSLLVA
                     GCYFSFSRGPWLGLALAFVILAFLGSTKIRSRLAIIGVIAGLILVVRPGVLETIGGFA
                     KDTADTDSFKGGTFQYRLELWRVAASEVSKSAWTMLFGYGPGAGSVMEIERELSYRGR
                     YQKIESWDNHFAYALFQSGYVGLLATLVLYLKALVPMLIAARNPGNPNRELQACLFAA
                     AFVQVWMMTNVLVFAKQLDYLFWTLVASGFVLSSRAEFSRSPSAAPMPEHESGNDVEP
                     SLSDVDCPQCKLTALSK"
     gene            complement(21696..22220)
                     /locus_tag="PKI07_03775"
     CDS             complement(21696..22220)
                     /locus_tag="PKI07_03775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HOC50201.1"
                     /translation="MRRRYGVTIGAYSYGCCFVPGTFPGKTVIGRYVSLASGISVVRR
                     NHPVSGISTHPFFFNSKLKWIPRDNLPIADLTIEHDAWVGERAFILPGCTRIGIGAVI
                     GAGAVVTKDVPNFAIVAGSPARLIRFRFSEEIQTAVIRSQWWTRPVESFQGVFEEMAS
                     PVDPKTSMHLLTPK"
     gene            complement(22399..23877)
                     /locus_tag="PKI07_03780"
     CDS             complement(22399..23877)
                     /locus_tag="PKI07_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOC50202.1"
                     /translation="MKKFLRGLQVGDGLRHRLFRGGAWLAIGTVADQGLRFLRNIILA
                     RLLAPEAFGLMAIVLSVCSLFQVLTGIGIKESVVQNPRGAERTFLNGAWWLSVARGLG
                     LYLLAFVSAPWIARFYEAPELERLLRVAFLSVLAQAALSAGAFVAVKQMRYSKWVLIQ
                     QGGGVIGVLTAITLGFYLRGVWALVLGFAVEGTARCLISYLVCPFRPGLQFDPENRRA
                     LLRFAGGMFGLPALMLVYTEGSTFALGKLGSKADLGIFAMALGLARMASMFSNQMVDL
                     LMPAFSEIQKDPGRVNQGILKVTTFLALAGVPASAFMALFGSQVMEALYGPRYAAGGL
                     ALALLFANEVLLVSSVPIATVYLAVGKPALLRRFSLLRAFLVVALLWPAIRFGGLTGA
                     ASTPVAAMLLAYGLQLAQLRTVTGLKIKTYLALWLRGLLVAAPFVGLWLVMRFFFQGI
                     RPLVELVWLGVATGVVLGTFAFSAYRQPSVRRYLWRFAHPAN"
     gene            complement(23893..24873)
                     /locus_tag="PKI07_03785"
     CDS             complement(23893..24873)
                     /locus_tag="PKI07_03785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC50203.1"
                     /translation="MTTPTLPSADLSIVIVNWNGGKLLNDCLASIVAHGGGLNLQVIV
                     IDNASRDGSREMAERAFPQFQVINSGANLGFGKANNLARSLVKSDFVLFLNPDTVLCE
                     NALERMVEFLRRNPNVGALGCKMLYPNGEVQELGLQYFPTPWKEFLHSLFMSTGTRQW
                     LKRWLPYHDPNHSGYAIKLYGGCLLCRKQALERVGWFDERYFMYAEDVDLCRAIRDVG
                     WKLYYLSEAKIVHVCGGASEKAPSGFSILMKSESIAKLIRKYQGGLGALLYRVGTLIA
                     AIVRLLILTLMSVVRLAARRPAHEELDTAFFKCRTLIGWALGVKRAQIAA"
     gene            complement(24870..25988)
                     /locus_tag="PKI07_03790"
     CDS             complement(24870..25988)
                     /locus_tag="PKI07_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOC50204.1"
                     /translation="MTPETASKASSTALNGYRVSVDETPEDEWNALLPLFDDASIYQT
                     WAYGAVRWGEKQLSHLVLWKNVTVVGMAQVRVIRVPLIGSGIAYIRWGPLYRLRGSSS
                     HGEALVALMEAIREEYANRRGLMVRILPNIFQGSTCAADFARICQEAGFAADEAHPSY
                     RTLLVSLEETLDQIRKRLDQKWRNQLNASERNGLSIEEGQSDELYARFLTIYDEMMGR
                     KRFETTVDARQFGRLQRSLPADLKMHILLCEKDGKNQAGLVSTSVGDTGIYLLGATSE
                     AGMKSKGAYLLQWQMIKRLKERGCRWYDLGGINPETNPGVYHFKAGLGGVESRQCSRR
                     ELSGSWISSACVMLPERLSGAIGAWRHRRKPALLNATK"
     gene            complement(25988..27847)
                     /locus_tag="PKI07_03795"
     CDS             complement(25988..27847)
                     /locus_tag="PKI07_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOC50205.1"
                     /translation="MGNFLLDLRPQAERELGPAADFLKFYPDMRVERLDYPEFGLVLT
                     SADKPGLWSAYQSPDGALMGALCGRIALDQKQWDKAATVEGSGGLACKHICQSYAAEG
                     IRGIENLNGNFVILLFDRPRRELHLVTDRWGLLPAFRLEGELAGRIFSSHPDVLADAA
                     GEGQNWDQVSFAEFILTGRLTAPYTYYRHIKALPVGSTTTLTFGENGGSEARTRCYFQ
                     PGKAGVVSASVEEVAERFAAAFRDSVTKRTLPLLGRTAIALSGGLDSRTVLCAAPDRA
                     GLVAFTCYDEENREFRIAREIARRAGVAFVPLKRHFDYYADHAAAGVRISAGMGCIAS
                     NHFLGFRDQLREMGGDNLLTGCYCDYIFKGLALNKQVNRWTTCESLGPFGFSFYAGHT
                     PADTELAGAVRERLEASIPAPLRRYNTESVVREVEQRRIFPLAYEEDNAERVIPQRVM
                     GWYPPVAENELMQVHLAMDTAMKLNRTLFGRMMRKICPSGILQVPDANTGVPVTASLL
                     REVFSCHLDRLGRFARKVIPTHSTMGSWLNWNSYILQSRKLQELWSEPNPVAMDVFVS
                     VLGKNRFHRSAGDYRGREVWVFLQLFTLKLWFDQRAKTRLPAPEPELGVCVQS"
     gene            complement(27840..28652)
                     /locus_tag="PKI07_03800"
     CDS             complement(27840..28652)
                     /locus_tag="PKI07_03800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOC50206.1"
                     /translation="MVKRLVKLGISLLVRGWDVLQLGVKRILGRASEPRCVVLYYHGI
                     ASSLKQAFIRQMDEAARLAEPVAAGFNGGLAPGRRYFAVTFDDGFVSTLENAFPVLLQ
                     RRIPATIFIPTGSLGQRPAWVKNPASPAWNETVMTADQVRSLRHYEGITVGAHSVSHP
                     NFQALEPAQAERELKESKTELETLMGCAVDLFSFPHGKYSAASLELAKAAGYRRVFSI
                     APEPAQGERASYVVGRVLVCPSDWPVEFRLKLLGAYRWMTWIQEGGPTPVHG"
     gene            complement(28672..29661)
                     /locus_tag="PKI07_03805"
     CDS             complement(28672..29661)
                     /locus_tag="PKI07_03805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC50207.1"
                     /translation="MSAYAIITPVRNEEDHFPHTLNSVCAQTVRPLAWVIVNDGSTDA
                     TPRLAEAAAGQHGWIRVVHRPDRGRRKQGGGVIEAFNDGYALLSSLFTKPQTVGADTP
                     PHSASTPPWSFLVKLDGDLQLPPDYFARCLERFADDSKLGIGGGMVCRQQNGDWEEDS
                     PGDPPFHVRGATKIYRRACWEALGGLVSAPGWDTLDELKANMLGWQTYTFREIKLLQL
                     KFTGAADGAWKNWVKNGLANYNTGYHPLFMLAKCLRRFISRPFSLAAPGLLAGFFGGY
                     LKGKPAGLDTQLVHYIRRQQMNCLLFRDSLWKPRGPGRAAAALPSPAGARAGH"
     gene            complement(29694..30734)
                     /locus_tag="PKI07_03810"
     CDS             complement(29694..30734)
                     /locus_tag="PKI07_03810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOC50208.1"
                     /translation="MRWDRSITLNVIRPWQRLFKAAPPSGAATAADRPGLAAGSSIPI
                     LMYHSISADSEPGVHSYYQVNTHPGVFRQHMQFLADNHYRTITLDQLADAAAHPAIGA
                     PAPQLPQTPPPGSTATSARAVVLTFDDGFRDFYTEAFPVLREFGFTATVFLPTACIGD
                     ERRQFRSASRNAQLSRGKECLTWAEVRELHSAGITFGSHTVTHPRLVELGWAEIRSEI
                     VDSKAEIERHLGETVTAFCYPFAFPQNNSPFVQRLAGLLRDTGYRCCATTQLGRWKAG
                     DRAYFLKRLPANSQDDPAFFAAKLDGAYDWLGTIQTLSKKLRRRQRPHREAPPKAAAP
                     TLAEAEAANSDS"
     gene            complement(30808..33069)
                     /locus_tag="PKI07_03815"
     CDS             complement(30808..33069)
                     /locus_tag="PKI07_03815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC50209.1"
                     /translation="MLRNRLYYSVKPLVPLPLRFAIRQWFALRKLRAVGDIWPIMPGS
                     ELQPQDWPGWPEGKRFALVLTHDVEGQGGLDKITRLMELEIQLGFRSAFNLIPEGSYR
                     VSRELRAQIEAAGFEVGIHDLYHDGKLFLTRSQFNRRAVRINHHLKDWAVTGFRSGFM
                     LHNLDWIHDLQVAYDASTFDTDPFEPQPHGQNTIFPFWVSRNGKPRAGVAPAASARAP
                     ESSTGPRPGYAELPYTLPQDSTLFLLLKERHPDIWYQKLDWIARHGGMALVNVHPDYM
                     RFPDERDTPHTYPADCYRQFLDYARQKYKDTFWQPLPRELAAFVARQRIPMRRAPRRI
                     AMVTHSHILSDARVLRYAEALAQRGDQVDVLALRRSREVPTHETLGNIRLIYLQPRYG
                     KTEKSPLSHLFPVLRFLASSSFWLLREHRRRPYDLLHIHNVPDFLVFAAWYPRLTGAK
                     VILDIHDIVPEFYASKFGTRPGAPAVRLLKIIERVSAAFANHIIISNHCWRDTYVARN
                     HLSGACSVFINNVDSRVFYLRPRTRHDGKIVVIFPGGLQWHQGLDLAIRAFRQVRSQI
                     PNAELHIYGDGNMKNRLVQLAAELELHDAVRFFDPLPIRQIAEIMANADLGVVPKRAD
                     SFGNEAYSTKIMEFMSLGVPVVVSRTRIDRLYFPDSTVRFFESGNVEALTEALLEVIQ
                     DPERRRRFIANGLEYVARHNWESRREAYLALVDALISGQPPAQVVPPDPFDQHPLPQT
                     EEARSPARELALK"
     gene            complement(33200..35422)
                     /locus_tag="PKI07_03820"
     CDS             complement(33200..35422)
                     /locus_tag="PKI07_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOC50210.1"
                     /translation="MQTHERPQHSTGLTLADIYYIIFRHKWKILSFSVLGLAVAAVVY
                     FTSPPIYVSEAKLLIRYVVESSMPSGIAGDPQIKNPDPRGDNIINSEVEVLQSLDLAT
                     QVVDIIGAGKILGAGPANTNKYQAAVLLQQNLKVEVPRRSNVLRIVFQNPNREVVQPV
                     LTQIIEAYLRKHVQIHRSMGGFDDVLTQQTDALRSQLAQTEQDLRKARTNIGVVPLEE
                     MKKAYSESLAKIRQELFNTQAELAQQQAVLEEHQKLLPKKPEASAAAPDAEVPPEKLE
                     SYRNIVARLDSFRKQEQGLLTQYTEENAMVRGIREQIAALEQTRQKLEADYPRLARLQ
                     PPSAKPGEAVGDFLEERARISIAALQTKIKVLNSQMDQLTAEAKAVNELEPGIVELQR
                     KKELEEAKYRHFSTSLEQARFDETLSAGRMSNIGVAQAPSPAFRESRQLVQMLGLIAL
                     GGVVAGFALAFLIELVLDRTVRRPIEVETRLRLPLFLTIPKMRRNSRPPLLPSPAPAA
                     GSPESGGADEKAAVDQKAGVVPYQANGLVSYYEALRDRLVTFFEVRNLTHKPKMIAVT
                     GCAKGVGVTTIAAGLAASLSETGDGNVLLVDMTAGQGAAHPFFKGKPGLGLAEALGNE
                     TRDNTMVQENLYAVAEGGKSGLLPRVLPRRFGSLVPKLKASDYDYIIFDMPPVSQVSV
                     TPRLAGFMDTVLLVLESEKTQSEAARRAAAMLAESKANVNAVLNKNRSYVPQRLQQEV
                     "
     gene            complement(35504..36577)
                     /locus_tag="PKI07_03825"
     CDS             complement(35504..36577)
                     /locus_tag="PKI07_03825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC50211.1"
                     /translation="MEILARGALLAHGRVVQRLNPIRDERWNDKLAAVAPDAFFPGKG
                     WALVLAQTYGYAPEYFAEQTGGRLEALLPVMEVDSWLTGRRGISLPFTDYCDPLAADA
                     AAFRQLFDAAVAHGRSRRWKYLELRGGQPFLAAAPPAQQYYGHELSLAGEEPELFAAL
                     KSATRRAVRKAQHEGVTVEFRSDVRGIRQFYALHCRTRRKHGLPPQPLRFFLQVQERI
                     LDHGDGIVVLARAGARYIAGAVFFQKGATALYKFGASDERTLRLRGNDLVIWEGIRHY
                     AARGCRKLYMGRTSPANEGLLRFKRGWGAREYPIPYYRYDLRKNAFVPGHDEAVGWHN
                     RVFGALPLSLSRLLGALLYRHVA"
     gene            complement(36865..37509)
                     /locus_tag="PKI07_03830"
     CDS             complement(36865..37509)
                     /locus_tag="PKI07_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOC50212.1"
                     /translation="MIIKAEQSFSAHRVSAGAVAPGWGPGAAGAPWGGGWGWYCAKTK
                     PKQDHIAAAGLRHRLGLEVFSPRLRSEQTTIRGVVKRITEPVFPGYVFVRCFLDEHIG
                     QIRHISGISGLVSFGGRIPQVPAEVLEDLRRCFGAEELLPLRKDPRPGDEVTVTRGAF
                     MGMQALVLQSWPARRRVQILLEILGRPTPVEMDRRQVSLPRDSVAEWLPELARA"
     gene            complement(37781..38470)
                     /locus_tag="PKI07_03835"
     CDS             complement(37781..38470)
                     /locus_tag="PKI07_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOC50213.1"
                     /translation="MKKLNESVWDGIAGRMWVGGLSLLLVVLLAGCQGPGAKQSANFA
                     EISPSGAVAASPASPSASPTAESLMLREGDTVRITFPGSPNLNTIQQIRRDGKLSLPL
                     VGECKAAGMSPTDLEKELVKLYAPQLVTKEVNVALESSAYFVFVTGAVARPGRIAFDR
                     PVTVLEAVIDAGVDYAKANLKDVTVIRRQGEREEQYHLNLKRHLQGQGGESFYLQPSD
                     IVFVRERFTWF"
BASE COUNT         8820 a        11622 c        11010 g         7855 t
ORIGIN      
        1 ggttcagcag agatgccgct gatttcgcta aacaggggaa cggcggggct atggtcgggg
       61 cggtcagggc tgtcctggct tggatggtat gcaggcgggc aggcgaacca ccccattgaa
      121 tcgttttggg ttagggaggc gagtattcgt cagccaaggc gccgatgctc ccctacccca
      181 cccggcaaag aggcacgcat ctgttgtccg gtgccttttc gacggctcgg gggtgatttc
      241 agcgctgctc cccgctgaag atttccccct gtgccctgcg gagtttctcc ttttgcctcc
      301 agtccgctcc ccggccaact caggcgttta aagccctttt tatcctggcc ggccatgctc
      361 cagcccggtg caaatccaca gaagtcgaac cgcctgccgc ccctgccccg ataatggcct
      421 ggaaagggga actcctccca gcccaggtcc cgccagggaa tatacccggc ggcataaacg
      481 tatctgccga gtttaagcaa ggcccactcg gagggcttgg gattccggcc cgactacttt
      541 agttccccca ggacacgttt ggcttcggcg gccaactggg gagcaagatt caagttgagg
      601 gcgcgattga gcgactgttt gctttcagcg gaggatttca accgatattg agccatcccg
      661 agatagtaaa agagttcggc gtcattgctg cgggcctgga ggctctgttt gagaaagcgg
      721 gcggcgtttg aataatcttc acggcgatag gcgaggatgc ccaacacgcg ggccagattt
      781 ggatcatcag gaaacgcttc gcgagctttc atggcaagtt cggatgcctt gggctcgtcc
      841 ggaaaatgat cgaagcagag gatggcaaga tcccgtgcgg ctggaacaaa caacgggttt
      901 atggccagaa cgtgatcaag ggtcttcctg gctgcctgaa agtccccttt tccttgcagg
      961 agcaactggt tgaccattaa agccggaatg taactcggat cggctttaag aacctccgga
     1021 agctgggccg cgacggttgc ccgctgggct gggtcgcgat aagcctgaag cagcgaccgg
     1081 aagcgtttgg cctcgtcggc ctggccaaac gtcgcggtgc ccttcaaggc attctgcagg
     1141 gccgtctcgg cggcggacaa ttgtcccagg ccatagctgg cacgggccaa atcataaaag
     1201 agagccggct ggtcgggaag tttcaacgcc gcatccttca gcaggctggc cgcccatttg
     1261 taatctccgg cttcacaggc gaggtggcca agtaattgcg aaatcccggc atcttcgggg
     1321 gcggcggcgt gcgcgtcttt ggcgtatccc agggcctttt cggaattttt caagctgccg
     1381 ggggcggcga gcagacgcgc gaggctggcc agaacgaagg cgttctttgg atttaccgca
     1441 agcgcttgct cgtaggtctt gagggccttc tccggcgcgc cgtttcggat atggatgccc
     1501 gccaggcgaa ccagtgcgat ggaatcattg ggatcttcgg ccaggcgttt ttcaagctgg
     1561 gtctgtgccg cgggatcaga agctttcgga tcaatcgcca ggatggccag attctgccgc
     1621 gctttctctt tgcccgaaaa gtttttgtcg cttttgacgg ccatttccag ggcgctgcgc
     1681 gcggctcctt cctggccaag cgcatagcga accattccca ggtgatattg aatctcaggc
     1741 tcattcggag cggccgtggc ggcttcggcg agcaaagtca gcgccttggg aaagtcgcct
     1801 ttgcggtaga aaacccagcc cagggtatcg gctatggagg ggtccgccgg atacaactgc
     1861 cgagcgcgtt cggccagccc ccgggccttg tcgacctggt ccaggttttc acagtagagg
     1921 taggcgaggt tgttgagggc cgggccaaat ttggggttgg ccgccagcgc tttttcgtaa
     1981 gcctcgcgcg ccgccggata gtttttgagg ctgtcctgaa tgatgccgat ctgcaactgc
     2041 gttggcgcgt cgttcgtccg ggcgacatag ctgttgagtt tatccagggc ttgctgcgtc
     2101 ttgccggagg aaaggtagat ccgggccagg agcatgtacg gggtctggag ctggggggcc
     2161 agctcgataa tcttaagcag ggtcgtttcg gcctgcgcga gatcctcctg ctggagatag
     2221 atcttggcca gcagcagttg caactcgggc accttggggc gccgctcgat ctgttcctgg
     2281 acgcgcttga gcgcggcttg cggctgcttg tcgtccaggt caaggtccac caggagctcc
     2341 agcggcggca ggaagcctgg cgcgagctcg agcgcctgat tgcaggcttt ccgcgcgtcg
     2401 ctcatctgtt tctgctggac caggatgccg gccatcagaa acggcacctg cggattccgg
     2461 ggaaagcgcg cttgcatctg gcgataaacc gccagagccg gatcgaattt cttttggccg
     2521 gcataagcag ccgcaagcag catgtggacc tggggcagct ccgggtcgtg tttcagcagt
     2581 tgctccaacg accgggcggc ggcggcaaaa tcccccttct tcatggacag ctccgcgagc
     2641 gccaggacgg cttccgggaa attcggatcc aatgccagcg catctttgag cgaggcttcg
     2701 gccttggcgt cttcgccgtt gagcagataa cagcgcgcaa gtaattgctg aattccggga
     2761 gagtgggaaa acatcttgga aagacgcagg agttcctcgg ttgcctgtga gacttcccct
     2821 ttctggagct tgacattggc tcccagcagt atcgcctgaa tgttataggg gtcccggacg
     2881 atgactttct gcaggaaggc cgagcattcc tcaagccggt tctgggtgaa ggcaaactgg
     2941 gcgaggtaga tccagggcgg caaataatcc ggcgccttgt gcgtgagctc ttcgagaatc
     3001 ttcaccgcct cattggtggc gccggttttc agcttgaatt cggcgtattt cagccgccgg
     3061 ggggagcgag gcggggaaag ttcggccccg gttttaaagg ccgcgtcggc gcgagccaga
     3121 tcgttgctca gccaatacaa ggtccccaaa gcggtgtgcg tcgcgggaaa cttgggatcg
     3181 agtgccagcg cctggtttaa ttcgggcgcg acctcggcga tttgcccctg gcggagataa
     3241 agggtggcga gggccaggtg atagccgggg cgatctttgt cttgctgccg cagggctttg
     3301 atttgccggg ccgtctcttg cgcctccttc aagctcgtgg tggtctcggc gagggcgaca
     3361 agcgcatcgg cttggccggg ttgcttttgc agcacgcgct ccgcgtccgc gcgggcttcc
     3421 tgacgcgccc ccagggcgag tttggtcagg cacagtttca gttgaaactc ggaatcatcg
     3481 ggggccaatt ccgtcgcctt gcgcaagtag gcaagcgcct gcggcagccg cccctgttcc
     3541 tggcaaataa tgcccaaata acctatggcg gttgaattct ctggtgctat ctggagagcc
     3601 ttgcggtaat ggatttcggc ctgctcgtac cggctggcgg caaagtccgc attcgcccgg
     3661 gagagatacc ggttttttct ggcttccttg gagcagccgg tggcgaaaag gagcaggagc
     3721 aggagggaga tggaaattcc ggggcccgtg aaggcgaaac cgcggaatat cctgaatggc
     3781 ttcatgggtg aaactgaaaa gcgaggcgtg gcggcgtcca gcttttcgcg ccccggagcc
     3841 gggccgccca aggccggaag tgaaggctct gacattcggg tgtgagtata atttttagac
     3901 atcgttagct tgaggttttc gcacatccgt cttgtgcaaa tagtagagta atagcaggaa
     3961 aggggccagt gaaataacaa agaaaaccgg gccgccgcgg cggtgtatgg ccgagtcaat
     4021 gaaaccgggg tcaatatgga tgcagagttc tccgatagtc acaatgcgca gggcattgcg
     4081 gaaaatgccc agcgggatca ccagaaaggc aaagagggcg cgtttccagg gcgtgcgcag
     4141 gaaaagatag cccgccacca ggctgctgat gaacagcacc agggtggatc ggatgccgct
     4201 gcattcagga gccacctgta atcggaaccc cggcagttgg aagtcaacgt catttctcag
     4261 cagcggggtg cccaccaggc caaacagcaa ctccgcggcg tccgccgagc gatattgcag
     4321 aaaagtttca atggcgtctt tgaccagcac cgggaaaggg acgcagaaga tgaggaacgc
     4381 cagcggaaag gccagcaccc gaagggtgga tttcccaagg caaaaaagaa cggccgccag
     4441 aaatagaaaa acaaaagata atgtcgtcca ggcaagcgaa tccacccggg cgagtttcag
     4501 cccccaatga ggcgcaaaat aatagcccgc caaaagcaac aggcctatgc ccagggggag
     4561 gacggcaagc cggcgatccg gctccgaagc gagcggcagt ttgtgccggt taagccaggc
     4621 caaatagagg ctgacaaccg ggatcagcag gatatgggag tagagttcgc tgtggagagt
     4681 aaagcgggcg agctcaaaca acggcctggc gaaacagacc gccagcacgg ctgtgcaaat
     4741 ggccaactga cggaactgcc tccggagcaa cggcgagagc attgcggcct gggcggcaac
     4801 cggaggttta tgggcttcag gatgaggttt caaaagatgg gggaatggaa cgggtggaga
     4861 tgaggcaaca aaacggaaat aatgacagcc atcggcaggc catggtttcg ggcgccctgg
     4921 cgacctctgt tttcggcccg aaatgcttaa cttctaatac cggcagctga gttgcagacc
     4981 gaccgacgtc gtggtgtagt cgtaatccgc ggcgccggaa gcgttgcgat ttaccgagta
     5041 aaagacggcc gcaaaccctc gtttcagaaa tggagtggag agactggcac tgaacgaggt
     5101 ggtttcgtag tcacgatgcg aggcgaccgg ggtggccgcg gaagcgtggt aggcactgtt
     5161 gccgtagcca ccgcttaaat cgaggtaaag cttgcccagg agccgctgat gcagccccgc
     5221 gctaacaaag gtgttttcgg ttacctggtc ttgatagtaa gagggttgaa cggcctggta
     5281 agccgtgatg aaaaaggtgg tcgtctcata cggggcatat tgaaccgaaa gcgagaagat
     5341 cgggctggct aaatcggacg cacccgagct gaggaactgc atgttgttaa caccgccact
     5401 cgccacaaac tcgagcttct tgcttacggc ccaggtaatt cgcccctgga tttgctctga
     5461 agtgatgtcc gaactggaag tgcagtcaat atactcgaat ccagcgccca ccgctgcctt
     5521 aatgccgggg gaaaccttgt aattcagcca actcgaggcc gaccaactgt taaaattatt
     5581 gagagccgcg cctttggaag agcgttcaat caagcggagg ttttgcccaa gccccaattc
     5641 cagggaagtt ttggaattga tttggtaatc ggcactgagg cgcgtggaaa gtgtttgctg
     5701 atcggtttgg cggctggtct caataagtgg ttcggaagaa gacatatagg attgagaaaa
     5761 tcccaacgtc cagtcctcat aggtagtacc gccgctcaga gtcacagaat gatcaacggt
     5821 attccggaag gcactgctgg aataaaatct cagggtgggg gtataatcaa gagcccagtg
     5881 agagccccat tgagccagaa accccggcgc caattcgtta atcacgcttt tggcttttcg
     5941 gccaggcatg gaccgaagcc cattcccata agaaacacga taaagcgcat gcgtttgaag
     6001 cgtcagcggc ccataacgga acaacggtgg gagagtcgag agaggaacag cagcgggcgt
     6061 gtacatctgg cgcgttcccc ccagtggcgg agcgggcgcg gcttccggaa catataccgg
     6121 cattggcaca ggaggcagat tggtctccaa agcctgcgaa taaacggatg gacttgtata
     6181 ttgaggaagt ggcgcgttcg tctcctgagc gtgtccctgg ggcaatgcgg cgacgacgct
     6241 taggacaacg gccagattcc aaaattgaaa gcgacgcacc tgccgcggtc tcggcagacg
     6301 gaaggttggt cgcggtagcg tttgataggg caatcgaatc attttattct cttgggcgtg
     6361 aggctaaggg ccatctggca ctagtttcaa gggtatttcc accaaagtgc tgcttgagtg
     6421 agagagcggc agaagctggg tctgattatc ttctttcgtc cgccttgcaa ccgtccacta
     6481 tcgttgtagc tctaatctcg ccagaggtgc cctatgggtt agagggaagc cgacggcaag
     6541 gagaagcagg tctgaaggtt tcctttcctc ccatttttga cccgcccccg gccatctctg
     6601 ggccgtgccc caccgtttgc cagcagggat accggtgcct accgcgcccc tataaaggcc
     6661 tgaaagggac cgactcccca accaaagtcc ctttggggaa ggcacgccac agcgtggagc
     6721 gcgtctgcgg ggttaagcaa ggcgtcactc gcggaaggct tggggcgggt gtctccaatt
     6781 gcatcaaggt ggttgggagc tggaaaagag gccaaagcca gaaatcccgc aataacgtat
     6841 tcttaggctt tgtctcctga ccccgctttt aaggtgttat taacttctct cacaaagttg
     6901 atgctggaaa atggtttcaa tagcctcgcg gtgagtctca cattggatga gattacggac
     6961 tccaagcagt ggacgcttgc catacaggag gcgaaaaagc aggccgtttc ggttcagctt
     7021 atgcataacg cgattgaacc cgagcaggct gccgatatac ccatgcttgt tttcttcgca
     7081 aaacttaacc cattccgctt ctacaaatct cttgtcgaga atcatgcgag atcgctctgc
     7141 caaacactca taaaaagcct gctcgcgctc tgggaccatc cgccttgctc ccggttccgg
     7201 atcggactgg gtgaaaggga taagggttag ctgagagctg ggagaattct gcgatttttg
     7261 gaagccggca gtagtgagtt gaacattgag ggtgagtttc acgagaaaac cctcatggaa
     7321 tgacttccga ttccggtaga tatcctcgtc catgagcagg gctccctgtc cataaacaat
     7381 atgcccgccc aggtagtttt catatccgcc caggatgtgc gggtgctgga caatgaccac
     7441 atccgctccc atctcaatca tgaagcggca ggtattttgg atgcgtggag tgggagcgaa
     7501 aaattcggcg gccccatgaa gaagcaccac caaaaaatca aagctcgcac gatgctcccg
     7561 aatattccgg acgaaagtga tgaggtccag cgggttggcg cccgggctat tttcccccgc
     7621 gatcgaaaat tcgtgttccg ccgccgccag caggcccact cgcaccccgc cgcaatcccg
     7681 cactacaatt ctcccggctg cggcgagatt tttccccgcc ccaaccgtct ccactccctc
     7741 ctgcgcacaa actttaagcg tgtttattag tcccgcacct ccatgatcca gaatatggtt
     7801 gttggccagg cataatactc cgatcccggc gttcttgatc gctttgatac agtcgcccgg
     7861 cgcgccgaaa atggggccgg ttttcatgat gggcgacggc tccccgatca gcggacactc
     7921 caaattggcg accatcaaat cggcccgctc aaattcgggc aacaagtcgt tcagcagccc
     7981 ccgggcatca ccggccatga aaagcggacg attattgcct atcggacaaa tatccgcagc
     8041 gatgagaaga gaggacatgt tcagtggagg aatgataagg ggaggcgatc cgtatagaat
     8101 ctaacgcggc tcttgcttcg tgatttttgt cacctcggtt aaaatccagg atggctgctg
     8161 tttagcgcca ggttgtcgtc gaggccggag tgttcttccc gtgagcgtcc gcccccaagt
     8221 ggtttcggtg cgcatcccac atttcgcgca caaactttgg cccccacaca tacccttgcc
     8281 gctggaaatt cccaaaagcc acgcgggtta aatccttgaa cccggcttct ttgatctgtt
     8341 ttacaaacga cgtatcatcc tccgtcatcc agcggtccac gtaagcaaaa aaggcatcgt
     8401 gattccacac gggttcggcg tgcatcatcc gtgccgccaa tgcctccgcc acccacgccc
     8461 cactagtatt cgcccggcga taatcttcgc tcgctggcaa atcattgggg tttttcttcg
     8521 gccattcagt gggcggaaac aggtccacgg caccccatcc gctctcccat tttccgcgat
     8581 caccccccgt gccggccaac gaatgcccag taaaaatcgc cctcgccccg gtccagccgg
     8641 cagcgtgcgt tttccctcga tattcgtagg gacagaaggc cgtctgatca tcttcgccaa
     8701 aatgaagttc cgggtgattt ttcgtcgggg actgcattgc ggagtccccg agcatcaagc
     8761 cgctaaagat aatgggccat ttgcgcccgc tgtgcagccc gccatgagcc ggccaggaca
     8821 tgccagcacg ggcggccccc caaagatcaa ttcccacctg cacgagattt accaatagtc
     8881 gttctttctc cggcccggga taatccatca acagcaataa agtggcttcg ccgactgcct
     8941 gggccatgtg ttgcccataa tgcgggagat tctccacggg ggccgcaaac ccgaactcca
     9001 ccgtgtcgag ccagggtttc tgaaacgctt tgatatatga atccagcaga ggaggcgcgc
     9061 tgggggggcg cggcactttc aacagcaact cacgccgcag gtctcgggcg cggaaaggag
     9121 ggctgtttgc cgaatcacag taagacggcc gaaacgcatc tgcgggtggc ggttcggcca
     9181 cgcacgagag cacggcggcc acccgcaaag ggtccacatg ttggccgccg aatttcgtaa
     9241 tctcatcgtt ccggcggctg atcgttgata ccagcgaatc gcccggcttc atgcgaatcg
     9301 gcaaatgcgc aaacagggtt ggatcatacc ggccactggg aatccggctg tcgtaaccgc
     9361 cctctcgctt tctcgccgga ggattgagca cggacccatt ccgagcctgt ttgccagggt
     9421 agttgctttc gcgcacctgg ccgcggtcta tcagttcccc cacggccttg ccccacaagg
     9481 gcgctggctc aatcgcggcc acgaccaccg gggcaaccac ataccagtcc ccattcacaa
     9541 actggcccac acgtcctggc tccgcaaacg tccatgtgat cccatactgg ctcacactgg
     9601 accggaggga ggcgtcctgc gctatgccag tcagcgccat aaaacccaaa aagcctgtcc
     9661 aggcaagaat gagaatccgg ccaacgcaaa accgcctgga taacgattca atcaacggtg
     9721 atttcacgga agacgggtgc tttgatcgca gtttcgtgcc tcaattcaac gattcgtccc
     9781 tggagctaaa cagctcttga aaagtccggt aaagtgtggc ttgggcttcc cgcactttct
     9841 ccgccagcgg ctcgcgcaat gcttcccgac gccggtaaat ttccatcgcc ttcgccactc
     9901 cctgctcggc tgtcaattcc cgtccgtcca cgacccaacc agccgcgcct accgagtcaa
     9961 acacccccgc gaattttttg ctgtaggcca ctcccactgc gggaatcccc tgggacaagg
    10021 ccgcgatgca ggaatgcatt cgcgacccaa caaaaaaacc gcactgcccg atcacccctt
    10081 taatctcgct ctgatcatat tcaccctcca ccaaatgcac ccgcggctgg agttccgggg
    10141 ataaccggcc gcgcaccttt cggcatgcct ccggatcact ttcgacgcta tgcgccggcg
    10201 cgaaagtatg cggcaccaaa agaacgcgga ccgccgggtc gcccagcaat gcctgcatga
    10261 gttgtgcgag aaactcggcg taatccagct tcaggccaaa catgttcttt cgggtatagc
    10321 caccgttgaa catgagaccg ttaacattta accctacgag ggtcgttgaa ggtgaagcgt
    10381 cggaggtgga gaggttcagt ggaggaatgg taagaaactg cttcggcggc cgggcttcca
    10441 gggcaaaggc cacgtctgga caaaaacggc agcggcttgt ttccccaatc agatccttga
    10501 cggttgccag actctcgcag tcacgggaca ggatcaccga agcacgacga agaatgaagc
    10561 gagccaaagt acgggccagc gacgttttgt acggaccata agtttgggga aaaagcgcaa
    10621 ttgttcctcg cactaaaatg acgggaagca cttccagcga tccttgcaga aagttcttca
    10681 gcccgtagat gtcgctgaag ctatcgccgc cgcgaatgtc tcccaccatg accgcttcag
    10741 tgcttgcgtg gataagcggg tgcttgcggc taaggaagtt ccgaacccat cccaccggca
    10801 cgacccgata caggacggaa aacaggaacc accaaacgag ctgttcggac atgcgggccc
    10861 ggggcgacag gcgaaagttg gcgatgggaa ttcgcagctt ccggtcgccc aggaacaggt
    10921 cgaagggctg gttggtgcgg gagccgatca gcaaggcagg ttctgccacc ggcgcggaca
    10981 aacgaaccag cttgaccagc gatgcgccca gggcggagac ccctcgattg ccagtcccca
    11041 gggacgcccc catcagataa acggtgcggc gaactggcaa tcccgctccc gcattcgggg
    11101 ttgcccttat agctttggct tcagcggctt ggacgaccat agaaacctgg gaaagcggtt
    11161 ttacccgaag atagcctaaa gaacatataa acggctaaga cggcgcagcg acactgcgca
    11221 cctcttccta cggttgcttc tgtaatacat gcacggatta attaccgctg acgaccgccc
    11281 ggtaaaagcg gatcggagta tttgtgggat cttcaatgac cataggattg tccgtgcaaa
    11341 gtaggttcgt aagtggattc cacaacgaca aatccggaga aaaatcaatt tggtaacgaa
    11401 tcccaagctg accaacgagc aggagtttgg gagattggcc gcctttcagc ggtgccatgg
    11461 tgagccttgg cgcggaatat ccaatattac gatttacgaa agctcccatg tcaatcccgc
    11521 ttcctggcaa taagagattt ccgttcgcgt cggtaatggg ggtgccgtcg aggtcgtgta
    11581 gcgaggggat accctggagc accgcaggat cgcccgcgca aacgcagggt gagtccgccc
    11641 gaagatggta gtccgcatgg tgaccgaggt cgtagcgctt gaggctgcgg ccatcggcgc
    11701 tcagaacgcc tctactgcgc aaataagcca gggcatcgga gagagtcatc acctttagat
    11761 tccccgagta atcggcaagg gcgtccagaa aaatacgaag gatttcccgc ggaataccgc
    11821 tctcattatg agaaacggtg cacatcatgc ccccaaattg acacagcata tccgccaagc
    11881 cttgcgtctt ataatatgtg ccattggaaa caacgacatc atccattaga ccgttataaa
    11941 agttgccgag ttgcccatca tccgggttgg ccgcgcaacc gatgtaaacc tgcccggtcg
    12001 cggccggaag gcgcagggtg tttgtggtgg tggcgaccac cacatcgttt acccggataa
    12061 accaacgatc gaacaattgc tgcaccgaaa tcttttggta ctggccgggg ctcactgctg
    12121 cgggcggagt cgaaaggcgg aaaatctctg cggcgtttga acgaacgacg aaattcaccg
    12181 ccaattgatc gttgacttca atcctcgctg cggtggccgc gttcgtaaaa gagaaaagcg
    12241 tgccggcgcc agtctgcccc cgcagccaaa agcggcctga aaaatgccag tcccgccgga
    12301 caaaattgaa atccgcattg gcgtctcgaa agacgcaggt gttagtgccg ttaaaatccc
    12361 cagctcgcga atggcgatag gcattcgtgg aatatgccag gttggcgcct gtaaaaccat
    12421 tggattgggg agaagtatcc tcggtattgc gctcaaaccg cagcatgaga acgtagccgt
    12481 aagttgttcc ctgcggaacg gtgatgtgat acaaatccat gttttccatg tgcgagaata
    12541 ccggggatgt ctcatccgcc cgcgccccaa gatagccggc ggcaatcacc tgcgacacca
    12601 ccgcatcgtc aaaagcgcct ccaggataga agaagctccg gcaggaatag ggcccgccat
    12661 tttcagcggt aaacgcagat tggatgtctt ccttcgaaag ccagatttca ttcgtccaga
    12721 aggcggggac attgtacggc aggagctgtt ccgcgagtgc gatatccaaa tcagtcaccg
    12781 tctccaacaa gcgggatggc accgcgtggc tataaatggt ggcgagccgg caggtatagg
    12841 cgggcggggc atcgagggtt gagaccagct cggatgtcgt gtccacgttt ccttgaagga
    12901 gattaatatc caagttttcg ccaccattgc ccccggaaac gattgttttg aggcggttgc
    12961 cttgaatcgt aagaaggcaa ttcgatcccg ttccgacgta ctttaaataa caacccaatg
    13021 ctgtggtcag aaaagcgtgt gagcgggaat gggatgcaat ttcgtgccct tgatcgatca
    13081 tggcttgcat ttttgtttta tcggaaggct ttagggtgtc cgtttttgaa agtgccagca
    13141 cggcccgcca ccccttggcc ttggccaggt ttgcaaaaac aatccagtcg tcgaggttct
    13201 cgtaatcatc cgttgccagg gacaagattg actcgcggtt caccgataca tagcgggggt
    13261 cctggtaaag gtttcggcca atttccgtga cattcaccat gtgtttcgaa ggattgtacc
    13321 cgttcgggag aatgagacta ttttggacca gaatcgggct gccggtgctc ttgatcgggt
    13381 cggaaccgct ggtgttggtc tggatggcat ggccggttag aatgcaattc gcgagactaa
    13441 cggcccccgt agcagcgcta gaaaccgagg ttcccgaaac cgagacgaaa ttacagtttg
    13501 cgaccaagac ggaggcggct gaaagaatct gaagcgcagc cggtacaaac tggttggcga
    13561 aaaggcaaaa gacaaacgac gcggaggttt gaggcccctc gatcagggcc atctggtttc
    13621 catagctacc ctcaaaccgg caggctcgaa acgcgatatt cacggaccga tttaggttta
    13681 gttggctgcc acactcaagc cattcagtat cgtcaaattg aatatcgttt gcatccgtta
    13741 cgctggcagc gtagctgctg gttttggtga agatcagatt tgaaacgata agcgaaccaa
    13801 ccgtattaag gcgcaaagca ttggggcgaa ccgcgcctgt cactgaaagc gttagggtgt
    13861 cagggttgcc cgcgtcataa cgcaggtaaa gttgcccgct ttcccacacc cacgcgccat
    13921 cgctcaatgt cccggcagaa gttcccttgg ttagatactg gttctccagc cacaggtaga
    13981 atacacgatt gggggcggcc aatctccagg tagccccacc cccaaatccg gcgaggctcc
    14041 agccggtgaa aacggcctcg ccagagatta ttggctttgc ccccgccccg taatcctgca
    14101 gacggatggg gtgcgccgca gtgcccgaag cggaaaacgt gaagttttca cgaaacacac
    14161 tcccgcgttt caactgcaaa tggacatcat cgccggcatt cacccatgcc tgtatcgcag
    14221 gaaaacttac cgcagatagg gtgcggaacg ggttgtccaa agatccgtcg cccgatcccg
    14281 ccgcctggga atcaaaatac acattgcgaa tggccccgct cgccgaaaac gcgagccaca
    14341 caaaaagaag gatgcgttca aacttcatat cttaggcagg agggggccgt gttgatcgtt
    14401 tttgtggacc caaaatcatt gcggaatcct ctcatttggg gatgccacca agaaccaagg
    14461 cgcgttgtca gtctgcggtc gaaatacaac agaaacggga ttatagcctt gttgatgagg
    14521 attcggttcc gcccaaacga gattcctata aaagcaggca ggccaggagt gttctgttta
    14581 gccggaacga ttcagatttc aactttagtt cgacctcaca gaacgtgtaa gtccctgatt
    14641 ttatgggcga acgtcattca gaatccagta gccatgccat tgaatccgcc aatcaaacgg
    14701 ttcgttgagg ctttccgctg gcctgtcatg tctgtttcga ctctaatgga gctagggcgc
    14761 acggttggac tatgcaacgt caacgtttca gataacgcat tcgtgaagct aaaagccatt
    14821 cgggcccccg cccacccttg aaaaactccc tcccgccagc cccagactta taactaactg
    14881 tttttactga gcttattagt cgccaccccg ccctctccca tcgtttcggt ctttccaggc
    14941 atccggccct attacccggc taccgtagaa ataccctgcc tctacgccgt gtacaagggg
    15001 tgtacaacgg atgctcatca ggagtttatg gggttttggc tggtacaccc gatgggcatc
    15061 ccttgtacac cccttgtaca cggcctgatg gcagggtaaa tccaaggcag ggctatagtc
    15121 cgccgaaggc ggcataattc aggctctgcg tctattttac ccatctctac acctaaacca
    15181 ttgattttac cggcgaaaga tccggaattc agcagccacg cccttgaatt tgcgaaataa
    15241 agtgttcgcc aaagctacaa cttgagcggt caggctcgct caagctccga tgaagcaagg
    15301 gcagacggta gaactgccca actgaatcgt tacgatttag gggaaatatc tacccgtcag
    15361 aattatcgaa aggtgtcgcc cttgcgcgat gcgattaagg aacttgccgg agccggaaaa
    15421 aggtcgccgg atttgtgtgg ccaaaagtcg caacaaacga tctcccttcg attgttggtg
    15481 ccacttccac cgcagaccag gggctggtca gcgtcaatgc ggattgcagc tcaaaggtat
    15541 tagccgaggt tggccaggaa agctggatgt tggtgggggg cgacagtgcg atagacaatc
    15601 gcggcagtgg gggccgggta ttgtaggccc ccaaatcaac caggccggct cctttcccaa
    15661 ctaacttgga atcagcccgc aatcgcaaac cgtcatcagc cgtgaacggc acgccatcgg
    15721 ggccaagcgg atcatccagg tttacgaatc ccggatctcc tccattgagt gttgggtctg
    15781 gctccgggaa cccggttttt gcgtcatagt ttggagcgct ccccgcgacg aaattattct
    15841 ccagggtcac ggtattcgtc gggccgagga aacggtacca tccgacttga tcgggagtag
    15901 tagggccggt ggcttgcccg caatccacaa agatgttgtt ccgaatcacc gcgttagtgg
    15961 cgtagtctcc aaggtcaaaa atcagcggat gtttttcgac ctggacaaga gcgtttgcct
    16021 ggcgggctac gcgcagaagg gtgttattct cgaacgtggt gtttggcata cgaatagaca
    16081 gccagcccac gtcaacaaac acgttatttt tgatccaaaa aggaccgctg acagttttgt
    16141 tatatttttg ggcgttaata acgcccatca tgttaatgac aaaattattg ctgaatgtga
    16201 ttcgctcata tttaaccggc caacaatcaa agacatcggg agaagggatg aaggttcccc
    16261 aaccgttatt ttgaaagaga ttacgaacga atttgtggtc cgcgcccatg aaaaagatca
    16321 ggtcccagcc attgttgtgc tcgaggatat tagcctcaaa aagatggttg ctgccctgca
    16381 caaaccaata ccgataagag aggttgctaa accgattgct ccgaaacagg caattgtccg
    16441 ttccgtcggc aaggtacagg ccatagatcg ccgaacccgt gaggtaggtg ggtttgggtc
    16501 cgtcggctgc tatggcggag gtcgcaaaca ccaacgaagt atccgttacc gagctgacca
    16561 aaaaggactt ctcgccgttg gcaagagcca cattggttgc gcggtaaaat tgcacggtca
    16621 tgcccggaac aaaaccagcg gccaggaacc cgcccgtaga agagctgatc ttattcggcg
    16681 gctcaaagac catgtcgttg cggacggcct gaatgccatc tcgaacagtg cagttgagca
    16741 gttcgaacca atccccggtc ttggaaacgg cgacgtaagc atcggagtta ctagcggagg
    16801 tagccccggt gatgtcaaag ccgtctactg ttataaatgg atgttgaacc gcaaaacctt
    16861 tcatcgtcac cgttccaatg gcacgaaagg taatgcggtt ttccggcgcg gtcccccccc
    16921 gaacagtggt gactcgctcg tcaaagtggc cagattggac ataaaccgtg tctcccgcac
    16981 tcatcgtatt ggcggccttt tggatcgtgc gccacggttg agcctcattg ccaggagagt
    17041 tatcccctcc gtttgtagat acgtaaaacg tggccgcggg accttgcggg gccgaaacca
    17101 caactagtgc cgttgctaga attaaacgcg tcaacatgcc tgttttgtaa ctgaagcgta
    17161 tgtagttaaa aaagcgtcca gaaccttgtt cttctccgtc accgcattaa aaatcgaatt
    17221 gcaaccaaag cctccattgg ggacgccaga aagcgagagg cagtcgcgga gcgatccgcc
    17281 gataccaaag cttgtgataa ccgaattcca tcgcctttcg gaaaaggata aaaatcgcca
    17341 gctttaatcc aacctgggca ccacagcggc gtacgcggaa ggtaaaggta ccatactctc
    17401 tagccccaag ccggattttg tagtcgtaat gcgccagtcc gccttccaaa tagcgtttac
    17461 cctcctcaat tcctctttcg atcagggtga ctaaccccgt cgggcctaga ctaaatcggt
    17521 cccatttcgg agcgatcgcc cgtgcgggca gttcccagta gtagcggtcg ccaaaggcat
    17581 aggtgtattg attggcaatc acttcttcat tcgccacgat gcgaacgaat cttacccggt
    17641 tcaaggctgc ttgtgcccgg acgagtgccc ggttgaactc caaagctttc ggccaggccc
    17701 cgaaatgccc cgtcttccct tccgctgccc attgggctgc atgctgttgc gcaaagcatt
    17761 caaactcgga ttccgccttt gaaagctcag taatgacttc aacccgggtt tcgtattcct
    17821 ttttcaacag gcgcaactca tactttcggc ggtttttacg ttcgttcttg ctcagcgccc
    17881 cgtaatatgc ctccatgttc gcaggcaact caaagacact atgcactccc cgcaagctct
    17941 caaggttgcg agtcaagtca tgtcgaagtt tacacgctct ttgtagtggt tcggccagat
    18001 ttaggcgctc cgaaatgggt cccaggctga caagatcgca accgtcttcc gccagtaatc
    18061 ttttgaagag ttgctcgaat atcgtctccg taaaggccgg gtcgagcggc ggatcaaaca
    18121 ctttcggagg tatgttggca cccaccaggc gcgcgacttt gagtcgtaat agtccaagac
    18181 gaacggtctc aatatagatc gggagcagtc ccacaatccg ttcattcacg cgccagacaa
    18241 agagccgcaa cgcctttcgc tccccataga attgccacca gatccgacac caatcgaaag
    18301 tcatgtaaat gctgcctccc aacctggcag cactgtcatc ccattgcggc tgcaaatgcc
    18361 ggacatcttc aaagctgcag aaaatctccc aggacaaagc actgccctcg gcacatgcct
    18421 cgatggaccg atcgttaaga agtacggtat tcaacaggtt ggctcaaata ccggggcgat
    18481 tccggccgtg ctccatcgag ggacggcggg actggctgga gaggcgtcgc gccggcactc
    18541 tagggatgaa cgactatacc cttaaaactc ggaggcaata ctactcggtt ttcggaagca
    18601 acgaattgca ttgcgccaat gtcccaattc ccagaaacgg gacgagcatt cccggtatga
    18661 tccctggaaa aatagttgct caaattgact cccttgtttc gcaaaggagc atcctgacgg
    18721 agccgaaagt catgttgtcg aatatcttgg aagcaatcac ttccaccgtt aatcccgtgg
    18781 ggctcatacc atcgctggtt agtttgaggc aacccggctc ttttgggact accgtccaag
    18841 gcggccacat aattaaaatc caccttccac gtattcgtta cgatactgga gccatcacca
    18901 accgaatacc aaccgctttg gtcattcttt ccgcacccaa ggaagacatt gttcagaatc
    18961 tgagcattcg tcgcattgtt gcggccactt tcggcccagt taatgatgat tgctccgcct
    19021 gacgcattcg tccagatttg gtaaaacaca ttgttgtgcc accgtactga tggaatcccg
    19081 cactctcctt ttaataatcc aaaaaagata ttgttgcgaa tatcccaatt gtgaatctca
    19141 tgattcagat tttgttccag ttgacaaatt tgcgctatat cgacgtccgt cgggttgtaa
    19201 ttgtaaccga atacgttttc ctcgataatc atgtgtttcg atgcatggcc gtttagtcca
    19261 aatgtttgga aacagtccat gtggtttccg ctgttggggg tatcctcgaa gccgatgcaa
    19321 atattccccc gcactgtgtt tgagactcca aatacatgga agtaatccgc atcccgaata
    19381 tcccgcatgc ggttgtttac gaccaaatta ttcgtcccca tcaagataat acaaacggtg
    19441 ttgtaaatgt gggcaatgga attgttcgta atagttaccc tgctcgccac attgggatca
    19501 atcggagtct gggacccgaa atcccaatta acagcccgga ctgaatctgc gtacgcagtg
    19561 tcaatttggt ttccctcaat ataggccccg tgagcgcccc ttcgcaggtc aatcgcgccc
    19621 gtaaatttag tagttagtcc ggtaagtata aagtttcgta tccggacatt tggcgatgct
    19681 atttggaact gggcgttcgt tgctaccccc tggccgtcaa acgttacgca ggcccctgct
    19741 gataccttat ggcagatttt gacgcactcc ccgtagttgc ctggacctat caaaaccgta
    19801 tctcctgctc cagcgacatc ggctgctttt tgaatggttc gccagggagc cgtctgcgcc
    19861 ccagagttgg cgtcatttcc ggaaagagcc acgtagtagg tggcggcctc taccagggga
    19921 tttccccaga gcatcagccc cccaaaaacg agcgggaaga gggctcgctt caagtccgta
    19981 tataatatta taatcccgca ggatcgaaaa cttaagcaca tcacgcgcat agccacaggg
    20041 tattacagca tgttctgttc cgaaaaaacc cgatccgttt ttgttcgcgg atgataagcc
    20101 ccgggtgatt cgtggaaaat cctgtagggt tttttgtcaa cattgtctgc tatttcgaca
    20161 gggcagtaag tttacactgc gggcaatcca catcggacag cgacggctcc acatcattgc
    20221 cgctttcatg ctccggcatc ggcgccgcag aaggtgatcg ggaaaactca gcgcgggacg
    20281 acaagacgaa ccccgaggcc acaagggtcc agaacaggta atccagttgt ttggcaaaaa
    20341 ccagcacatt ggtcatcatc cagacctgga cgaaggctgc ggcaaagaga caggcctgca
    20401 attcacgatt gggattcccg ggattgcggg ctgcaatcaa catcggaacc aaggccttca
    20461 ggtaaagcac cagggtcgct aacagcccca catatccgga ttgaaacaaa gcataagcga
    20521 aatgattatc ccagctctca atcttctggt aacggccccg gtaggaaagt tctcgctcga
    20581 tctccatcac gctgccagct cccggcccat agccaaacag cattgtccag gcagatttgg
    20641 aaacttcgct ggcggccaca cgccatagtt ccaaccggta ttgaaacgtg cctcctttga
    20701 acgaatcagt gtcagccgtg tctttggcaa aaccaccgat ggtttccagc actcccgggc
    20761 ggacgactaa aatcaagccg gcgattacgc cgataatcgc gagccgcgac cgaatttttg
    20821 tggagcccag gaaagccagg atgacgaatg caagcgccag tccaagccac ggaccgcggc
    20881 tgaaagaaaa ataacaccca gcaaccaaga gactcagcgt tccccaaaaa accgcacctc
    20941 cttcgtccac tcgcttccgg gaacgcagcg gcagaaagct taacggcacg cccatggcca
    21001 gcgcagttcc cagcaggatc cgatgctgaa atgttgataa gatatcgcgg aattgaacat
    21061 cttcccgggc ataaccgggg atatagcggt ccactggatt aaacccggta tagcgttcaa
    21121 taaccgcaaa caccgcaact acgagcaggc ccagccaggc agcgcgcaaa acccggagca
    21181 cgtcttcgcg ggtcttcagg cttgttgccg ttatgatgta ggcagcaaat acttcgagca
    21241 cgaagtccaa cacccgcttc aagctaatga caaaatcaat ttgggtcccg gcaagggaca
    21301 ccaggttagc catggcccaa aaagcaaaat agccgagcaa cggcaccgac gctaatttgg
    21361 ccatccctcc cttgcgaatc caggcaatca ggaccaaaat caggagcagg cggtgaatcg
    21421 tgagttctgg taatgagccg ggcagatcaa tccgcaggaa tgtggtcatc gaaacccaga
    21481 tgaacatcgc gcaacttaat ccccggataa agtcccgctg gagttgaagc cagactacgg
    21541 cagccagcag caaaatgaat acgattatgc cgattgaaga cacaactcag aaaagcttgg
    21601 aaacctattg aaccacggtt gtttttcttt gtccgtgttc cgttagtttt ttccggggaa
    21661 ccgccccgtg cccgtgaaaa tctttctcaa gtatttcatt taggcgtcag caaatgcatg
    21721 ctggttttcg gatctacggg gcttgccatt tcctcgaaga ccccttggaa gctctcgacg
    21781 ggccgggtcc accactggct gcgaatcacg gcagtttgaa tctcctcgct aaagcgaaat
    21841 cggatcaatc tagcgggact tccggcaaca attgcgaagt ttggcacatc cttggtcacc
    21901 acggctccgg caccaataac cgccccgatg ccaatccgag tgcatccagg cagaatgaag
    21961 gcacgctctc ccacccaggc atcgtgctca attgtaagat cggcaatagg cagattgtcg
    22021 cgaggaatcc atttgagttt gctattaaag aaaaatggat gcgtagaaat cccgctcacg
    22081 ggatgattcc ggcgaaccac tgaaatacca cttgctaaag acacgtagcg cccgatcaca
    22141 gtcttcccgg ggaaggtgcc gggaacaaaa cagcagccat aactatacgc gccaatcgtc
    22201 accccgtagc ggcgtcgcat aatttcgcgc gcagtctcag agtaaaactg gcctcgttcc
    22261 agccgcaggg cagccatgag acacagccga cgaaccttcg gcacctttga caagaggacc
    22321 agtaacccgc tcaacgagca ccggttcaat tcgtgagcgc aacgaggcgc atttttgtgg
    22381 gaatggttca tgggactatt agtttgccgg gtgtgcaaat cgccagaggt atcttctcac
    22441 agaaggttgc cgatacgcgg aaaaagcgaa cgtgccaagg accacgccgg tggcaacgcc
    22501 cagccaaacc aattctacca acggccgaat accttggaaa aaaaacctca tcaccagcca
    22561 taatccaaca aatggggctg caaccaacaa cccgcgcaac cagagagcga ggtatgtctt
    22621 gattttcagc ccggttacgg ttcgcaactg cgccagttgg agcccgtagg ccagcaacat
    22681 cgcagccacg ggagtgctcg ctgccccggt caacccaccg aaccgaatgg cgggccatag
    22741 cagcgcgact accagaaatg ctcgcaaaag cgaaaagcgg cggagcaggg cgggtttgcc
    22801 taccgccaga tataccgttg caataggtac gctgctgacc aatagcacct cgttggcaaa
    22861 taacagagcc agagccaacc ctcccgcagc atacctgggg ccgtagagcg cctccatcac
    22921 ctggctgcca aataaagcca tgaaggcgct tgccgggacc cctgccagcg ccagaaaagt
    22981 ggtcactttc aatatgcctt ggttcactcg cccgggatct ttttgaattt cggaaaaagc
    23041 gggcatcaac aggtccacca tttgattact gaacatggag gccatacggg caagaccgag
    23101 ggccatggca aatattccca aatcagcttt ggaacccagt ttacccaagg caaacgtgct
    23161 gccctcggta tagactaaca tgagcgccgg caagccgaac attcctcccg caaaacgcaa
    23221 gagcgcccgg cggttttccg ggtcaaactg caaaccaggc cggaaggggc aaaccaagta
    23281 ggaaatcagg caccgggcgg taccttctac ggcaaagccg aggaccaagg cccatacacc
    23341 gcgcaggtaa aaccccaagg tgatggcagt aagcacccca atcacaccac ccccttgctg
    23401 aatgagaacc cacttggaat agcgcatctg cttgactgct acgaacgccc ccgcactgag
    23461 ggcagcttgc gccaggacac tgaggaaagc cacgcggagc aggcgctcca actcgggagc
    23521 ctcgtagaag cgcgcgatcc agggcgccga gacgaacgcc agcaagtata gcccaagccc
    23581 gcgcgcgacc gatagccacc aagcgccatt aaggaacgtg cgctcggccc cacgcggatt
    23641 ttgcaccact gactccttga tcccgattcc ggtaagaacc tggaacaggg agcagacaga
    23701 gaggacgatg gccatcaacc cgaaggcttc cggcgcgagg agccgggcca gaatgatatt
    23761 gcggagaaac cgcaggcctt ggtcggcgac ggtgccaatt gccagccagg cgcctcctcg
    23821 aaagaggcga tggcgcagcc catctccaac ttgcaagccc ctaagaaact tcttcatcgc
    23881 gtcatgccgc atttatgccg cgatctgcgc ccgcttcact ccaagcgccc acccaatcaa
    23941 cgtgcggcac ttaaagaacg ctgtatccag ttcttcgtgt gccggccgcc gcgctgccaa
    24001 tcgcacaacg ctcatcaggg ttaatatgag cagccgcact atcgccgcta tcaacgtccc
    24061 gacacgatat aaaagcgccc ccaacccacc ttgatacttc cggattagtt tcgcaatgga
    24121 ttcggacttc atcaggatcg agaatccgct gggggctttc tcgctggcgc cgccacacac
    24181 atgaacaatc ttcgcctcgc tcaaatagta gagcttccag ccgacgtccc ggatcgcccg
    24241 gcaaagatct acgtcctcgg catacataaa gtaccgctca tcgaaccagc caacccgttc
    24301 gagcgcttgc ttgcgacaca ggaggcaccc gccataaagt ttgatggcgt agccgctgtg
    24361 gttgggatca tggtaaggca gccaccgttt gagccactgc cgggtccccg tggacatgaa
    24421 cagggaatgg agaaactctt tccagggcgt cggaaaatac tgcaagccca attcctgcac
    24481 ttcgccgttc gggtacagca ttttacagcc gagtgcgcca acattgggat tgcggcgaag
    24541 aaactcaacc atccgctcga gcgcattttc gcatagaacc gtgtccgggt ttaagaacag
    24601 gacgaagtcg gacttgacca gcgaccgggc cagattgttg gccttgccga aaccaagatt
    24661 ggccccggaa ttaatgacct gaaactgtgg gaaagcgcgt tcggccattt cccggctgcc
    24721 atcccgcgag gcgttatcaa ttacaatgac ctgcagattc agtcccccgc cgtgagcgac
    24781 gatggaggca aggcagtcgt tcaacaactt tccgccattc cagttgacga tgacgattga
    24841 taaatcggcc gacggaagcg tgggcgttgt catttcgtcg cgttgaggag cgcaggtttc
    24901 cgccggtgac gccatgcccc gatggcaccg ctgaggcgtt ctggcaacat cacacaggcg
    24961 ctgctaatcc aactcccgct aagttcccgc cgggaacact ggcggctttc tacgccgcct
    25021 aacccggcct tgaaatggta aacccccggg ttcgtttccg ggttgatgcc ccccaaatca
    25081 taccagcggc aaccgcgttc tttcaatcgc ttgatcatct gccactgcag gaggtaagct
    25141 cctttgcttt tcatccccgc ttcactggta gctccgagca ggtaaatgcc tgtgtcgcca
    25201 acgctcgtgc tgactaagcc ggcttgattc ttcccgtcct tttcgcacag gagaatatgc
    25261 atcttcagat cggccgggag gctccgctgc aatcgcccaa actgcctggc atccacagtc
    25321 gtctcaaatc gtttccgacc catcatctca tcataaatgg tcagaaaccg ggcatacagc
    25381 tcgtcgctct gcccttcctc gatgcttaac ccgttccgtt ccgacgcatt caattgattg
    25441 cgccatttct ggtccagtcg cttgcggatt tgatcgagtg tttcttccag gctgacaaga
    25501 agggttcggt aggaagggtg cgcctcgtcc gcggcgaagc cggcctcctg gcagatcctg
    25561 gcaaaatcgg ctgcgcaagt gctgccctga aaaatattgg gcaggatacg aaccatcaac
    25621 ccacggcggt ttgcgtactc ttcccggatt gcctccatca gagcaaccaa cgcttcgccg
    25681 tgggagcttg agccacgcag acgatagagc ggcccccagc gaatatacgc gatcccgctg
    25741 ccaatgagcg ggacgcgaat cacccgaacc tgcgccatgc caaccacggt gacgttcttc
    25801 cacaagacca gatggctaag ctgtttctct ccccagcgga ccgcgccgta ggcccaggtt
    25861 tgataaatac tggcatcgtc gaacaacggg agcagcgcgt tccactcgtc ctcaggcgtt
    25921 tcgtccacac tgacgcggta gccgttcaaa gcggtgctcg atgccttgct ggcagtttcc
    25981 ggggtcatta tgattgcacg caaacgccga gttcgggttc cggcgcgggc agtcttgttt
    26041 tggcacgctg gtcgaaccat agcttcagcg tgaataattg cagaaacacc cagacctccc
    26101 tgccacggta atcacccgcg ctccggtgga agcggttctt ccccagaact gacacaaaaa
    26161 catccatcgc tacgggattc ggttccgacc acaactcctg taatttccgg ctctgcagaa
    26221 tgtaggagtt ccagttgagc cacgacccca tcgtagaatg tgtgggtatc accttgcgcg
    26281 caaacctgcc aaggcggtcc aagtggcaac tgaacacttc ccgcaacagc gaagctgtta
    26341 cgggcacccc ggtgttcgcg tcggggactt gcaggatgcc cgacggacag attttccgca
    26401 tcatccgccc aaacagggtg cggttcaatt tcatggcggt atccatcgcc aggtggacct
    26461 gcataagttc gttttcagcc acgggaggat accagcccat tacgcgctgc ggaatcaccc
    26521 gctccgcgtt gtcttcttca taagccagcg ggaagatccg gcgctgctcc acttcccgca
    26581 ccaccgactc ggtgttataa cgccgcaaag gagccggaat ggaggcctca agccgctccc
    26641 gcaccgcgcc ggccaattcc gtgtcggccg gcgtatggcc cgcataaaat gaaaagccga
    26701 acggtcccag agattcacac gtcgtccagc ggttaacttg tttgttgagc gccagccctt
    26761 tgaagatgta atcacagtag cagcccgtca gaaggttatc tcctcccatc tcccgcaact
    26821 ggtcgcggaa tcccagaaaa tggttgctgg cgatgcagcc catgcctgcc gaaatcctca
    26881 cccccgccgc ggcgtggtcc gcataataat caaaatgccg cttgagcggg acaaaggcaa
    26941 ccccggcccg gcgggcgatc tcgcgcgcga ttctgaattc gcggttctcc tcgtcgtaac
    27001 aggtgaaagc caccaaaccc gcccgatcgg gcgctgcgca aagcacggtc cgggagtcca
    27061 atcctccgct caaggcaatc gcggtacgtc cgagtaatgg cagcgtccgc ttggttacag
    27121 agtcacgaaa agcggcggca aatcgctcgg ccacctcctc cacactggcg ctgacaaccc
    27181 cagccttgcc gggttgaaaa tagcatcgcg ttcgcgcctc ggatccgcca ttttctccaa
    27241 atgtcagcgt cgtggtgctc cccacaggca gggctttaat gtgccggtaa taggtgtagg
    27301 gggccgtcaa tcggccagtg agaataaact ccgcaaagga aacctgatcc cagttttgtc
    27361 cttccccggc ggcgtcggcc agcacgtccg gatgcgacga gaaaatcctg cccgccaact
    27421 ctccttcgag cctgaatgcg ggcaataaac cccagcgatc ggtgaccagg tgaagttccc
    27481 ggcgtgggcg gtcgaacagc aggatcacaa aattgccgtt taggttttca atcccgcgga
    27541 tgccttccgc ggcatagctt tggcaaatat gcttgcaggc gagcccgccc gatccctcaa
    27601 ccgttgccgc cttgtcccat tgcttctgat ccagcgctat gcgcccgcac aatgctccca
    27661 tcaaggcgcc atcaggcgat tgataagcgg accaaagccc gggcttgtcg gccgaggtca
    27721 gcaccaggcc gaactcagga tagtccagcc gttccacccg catatcggga tagaacttga
    27781 ggaaatccgc ggccgggccg agttcgcgtt cggcctgcgg ccgcaaatcg agcaaaaaat
    27841 tacccatgga ccggagttgg ccctccttcc tgtatccatg tcatccagcg gtaggcgccc
    27901 aacagcttca atcgaaactc cacgggccag tcgctgggac agaccaggac gcgccctaca
    27961 acataacttg ccctctcccc ttgagcaggt tccggcgcaa tcgaaaaaac ccgccggtag
    28021 ccggcggctt tggccaattc aaggctggcg gcggagtact ttccatgcgg aaagctaaac
    28081 aaatccacag cgcatcccat aagcgtttcc agctcggttt tgctctcttt caactctcgc
    28141 tccgcctgcg ccggttcaag cgcttggaag ttcggatgcg agaccgagtg cgctccaacg
    28201 gtaatcccct cgtagtgccg taatgaacgc acctggtcgg ccgtcatcac ggtctcattc
    28261 caggccggag aagcaggatt ctttacccag gccgggcgct ggcccaggct cccggtgggg
    28321 atgaaaattg tggccgggat gcggcgctgc agcagcacgg gaaatgcatt ctccagggtg
    28381 ctgacaaagc cgtcgtcgaa tgtgacggcg aaataacgcc ggcctggagc caacccgccg
    28441 ttgaaacccg cggcgaccgg ttccgccagc cgtgccgctt cgtccatctg ccgaataaac
    28501 gcctgcttca ggcttgaggc gatgccatga taatacagaa ccacgcatcg cggttcgctt
    28561 gctcgcccga gtatccgctt cacccccaac tgcaagacat cccatccccg cacaagcagg
    28621 ctgattccca gtttgaccag gcgcttaacc acgtccaatg cctcgtttgg attaatgccc
    28681 ggcgcgggcg ccggcagggg atggcaaggc agcggccgcg cggccaggtc cgcgcggttt
    28741 ccacaagctg tcgcggaaca acaggcagtt catttgctgc cggcgaatgt agtggacgag
    28801 ctgcgtatcc agcccggcag gtttgccctt caaataaccg ccgaagaagc cagccagcag
    28861 gccgggagcg gcaaggctga acgggcggct gataaaccgc cgcaaacatt tggcgagcat
    28921 gaacaagggg tgatagccgg tgttgtagtt cgccagcccg ttcttgaccc aattcttcca
    28981 ggcgccatcg gcggcaccgg tgaacttgag ctggagcagc ttgatctctc gaaacgtata
    29041 ggtttgccag cccagcatgt ttgccttcag ctcatccagc gtatcccagc cgggagcgga
    29101 taccaggccg cccagggctt cccaacaggc tcgccggtag attttagtgg ccccgcggac
    29161 gtgaaacgga gggtcgccgg gagagtcttc ctcccaatcg ccgttctgct gccggcacac
    29221 catgccgccg ccgataccca gtttggaatc atccgcaaac cgttccaggc accgcgcgaa
    29281 gtagtccggg ggaagctgga gatcgccgtc cagtttaacc agaaaactcc agggaggcgt
    29341 ggaagcggaa tgcggagggg tgtcagcccc tacggtttgg ggcttagtga acaggcttga
    29401 aagcagggca tagccatcat taaaggcttc aatgaccccg ccgccttgct tgcggcggcc
    29461 acggtcaggg cgatggacaa cccggatcca gccatgctgg ccggctgcgg cctcggcgag
    29521 tcgaggggtg gcatccgtcg agccgtcatt cacaatgacc caggcaagcg gccgcacggt
    29581 ctgggcgcaa acggaattga gggtgtgcgg aaagtggtcc tcctcattgc gcaccggcgt
    29641 gatgatggcg taagcggaca tgcgagtggt tgaccgagcc tcccggccaa agatcaggag
    29701 tccgaattcg cagcctcagc ctcagccagc gtgggagcgg cagcttttgg cggcgcttcc
    29761 ctgtggggcc tttgccgccg acgaagcttt ttgctcaacg tttgaattgt gccgagccaa
    29821 tcataggccc cgtcgagttt ggccgcaaag aaggcgggat catcctgaga gttcgccggc
    29881 aaccgtttta agaagtaagc gcggtctcct gccttccaac ggccgagctg cgtcgtcgcg
    29941 cagcagcggt agccggtgtc gcgcagcaac ccggcaagcc tttgcacgaa ggggctatta
    30001 ttctgtggga acgcgaaggg atagcagaac gcggtcaccg tttcgcccaa atgccgctca
    30061 atctcggcct tggaatcgac gatttcagat ctaatctccg cccaacccaa ttccacgagc
    30121 cgggggtgcg tcacggtgtg ggagccgaat gtgatgccgg ccgagtgaag ctcgcggact
    30181 tcggcccagg tgagacattc tttgcccctt gagagttgcg cattccggct tgccgaacgg
    30241 aattggcggc gttcatcgcc aatgcaggcg gtgggcagaa aaacggttgc ggtaaaacca
    30301 aactcccgaa ggaccggaaa ggcctccgta tagaaatcgc gaaatccgtc gtcgaacgtc
    30361 aaaacgaccg cgcgggccga cgtggcggtt gaacccggcg gcggagtctg ggggagttgg
    30421 ggggcgggcg cgccaatcgc cggatgcgcc gccgcgtccg cgagctgatc cagcgtaatc
    30481 gtgcggtagt gattatcggc caggaattgc atgtgctgcc gaaaaacccc gggatgcgtg
    30541 ttgacctggt aataggaatg aacgcccggc tcggaatcgg cgctgatgct gtgatacatc
    30601 agaatgggaa tcgaactgcc cgccgcgagg cctggccggt ccgcagccgt ggcggcgccc
    30661 gaaggaggag ccgctttaaa cagccgctgc caggggcgaa tgacgttcaa ggttatcgaa
    30721 cgatcccagc gcatggaaaa acaaccatca gcggggggca aggcagcagt gccgctcggg
    30781 aacgattcaa tgggaacgaa gcgagcttta ctttagcgcc aattcccgag cgggactccg
    30841 ggcctcctcg gtttgcggaa gcgggtgctg gtcaaagggg tcgggcggaa ccacctgggc
    30901 cggaggttgg ccggaaatca gggcatctac gagcgccagg taggcttccc ggcgggactc
    30961 ccagttatgg cgcgcgacgt attccaaacc gttggcaata aagcggcggc ggcgctcggg
    31021 atcctggatg acttccagca gcgcctcggt gagtgcctca acattcccag actcaaaaaa
    31081 gcgcacggtt gagtccggga aataaagccg gtctatccgg gtgcgggaaa cgaccacggg
    31141 aacccccagc gacatgaatt ccattatctt ggtgctgtag gcttcattgc caaaggaatc
    31201 ggcccgcttg ggaacaaccc caagatccgc gttcgccatg atttccgcaa tttggcgaat
    31261 cggcagcgga tcaaagaagc gcacggcgtc atggagctcg agctcggcgg caagctgcac
    31321 caggcggttc ttcatgttgc catcgccgta gatatgcagc tcggcgttgg ggatttggct
    31381 gcggacctgg cgaaaggccc ggatggccag gtccaatccc tggtgccact ggaggccgcc
    31441 ggggaagatc acgacgattt tgccatcatg gcgcgtccgc gggcgcagat agaaaacccg
    31501 cgaatccacg ttattgatga aaaccgagca ggcgccgctc aaatggttgc gcgcgacata
    31561 ggtatcgcgc cagcaatgat tggagatgat gatgtggttc gcaaaggcgg cggaaacccg
    31621 ttcaatgatc ttgagcaggc ggactgcggg ggcgcccggg cgggtgccga atttactggc
    31681 gtagaattcc gggacaatat cgtggatatc caggatcacc ttggctcccg tcaaccgggg
    31741 ataccaggct gcaaacacga ggaagtccgg aacattatgg atatgcagca aatcgtaggg
    31801 gcggcgccgg tgctcgcgca ggagccagaa cgatgagctc gccaggaacc ggagcacggg
    31861 gaaaaggtgg gacagcggag atttctccgt cttgccgtag cggggctgga ggtagatcag
    31921 gcgaatattg cccagggttt cgtgcgtggg aacttccctg gaacgccgga gcgccagcac
    31981 atccacctgg tcgccccgct gggcgagcgc ctccgcgtaa cgcaacacgc gcgcatccga
    32041 caggatgtgg gaatgggtaa ccatggcgat gcggcgcggc gcgcggcgca tcgggatgcg
    32101 ctggcgggcc acgaatgcgg ccagctcgcg gggcaggggc tgccagaacg tgtctttgta
    32161 tttctggcgc gcgtaatcga gaaactgccg gtagcaatcc gccggatagg tatgcggggt
    32221 gtcgcgctcg tccgggaaac gcatgtagtc cggatgcaca ttcaccaggg ccatgccgcc
    32281 atgccgcgca atccaatcga gcttttgata ccagatatcg ggatgccgct cctttaaaag
    32341 caggaacagg gtcgagtctt gcggcagcgt gtagggaagc tcggcgtagc cggggcgcgg
    32401 cccggtcgaa gattccggag cccgggcgga agccgccggg gcaactcccg cgcgcggttt
    32461 cccatttcgc gacacccaaa agggaaagat ggtgttctgg ccgtggggct gcggctcgaa
    32521 gggatcggtg tcgaacgtcg aagcgtcgta ggcgacctga agatcgtgaa tccagtcgag
    32581 gttgtgcagc atgaagccgg aacgaaaacc ggtcaccgcc caatccttga gatgatggtt
    32641 gatccgaact gcgcgccggt tgaactggct ccgggtaaga aataatttgc cgtcgtggta
    32701 caggtcgtga atgcccactt caaacccggc ggcttcaatt tgcgcgcgca actcgcggga
    32761 aacacgatac gacccctccg gaatcaaatt gaacgcggac cgaaacccca attggatttc
    32821 gagctccatc aaccgcgtga tcttgtccaa cccgccttgc ccctcgacgt catgggtaag
    32881 aaccagggca aatcgtttgc cttccggcca gccaggccaa tcctggggct gcagctccga
    32941 gccgggcata atcggccaaa tgtctccgac cgcgcgaagc tttcgcagcg caaaccactg
    33001 ccggatcgca aaccggagcg gcagcggcac gagcggcttg acggagtaat acaggcggtt
    33061 gcgcagcatt ttaacagtgc cgacagcgcc cggcagcccg atcccggatc aaatcggcgc
    33121 gcgcaaggac cattgccggc aaacccaaat cgtttaagct aaaaaccacc ggccgggagc
    33181 agggaagacg gagggccgct caaacctcct gctgaagccg ttgcggaaca tagctccggt
    33241 ttttattcag caccgcgtta acgttcgctt tggattcagc gagcatggcg gcggcgcgcc
    33301 gggccgcctc actttgagtc ttctcggact cgagcaccag cagcaccgta tccatgaacc
    33361 ccgccagccg cggggtcacg ctaacctggc tgaccggagg catgtcgaaa atgatgtagt
    33421 cgtagtcgct ggccttgagc ttgggcacca ggctgccaaa gcgccgcggc aaaacgcgcg
    33481 gcagcaggcc gctttttccg ccttccgcga ccgcgtagag attctcctgc accattgtat
    33541 tatcgcgcgt ctcattgccc agcgcctcgg cgaggcccag cccgggtttg cccttgaaga
    33601 aggggtgggc cgccccctgt ccggcggtca tatccaccag cagcacgttc ccatccccgg
    33661 tttcggataa gctggccgcc agacccgcgg caatcgttgt gacccccacg cctttcgcac
    33721 aacccgtgac cgcaatcatc ttgggcttgt gcgtaagatt gcgcacctcg aagaaggtca
    33781 ccagccggtc gcgcagggcc tcgtagtagg aaaccaggcc gttcgcctgg taaggcacca
    33841 ctccggcttt ctggtcaact gccgcttttt cgtccgcccc gccgctttcc ggcgagccgg
    33901 cggccggcgc cggcgacggc agcaacggcg ggcggctatt tcgccgcatc ttaggaatcg
    33961 tcaggaagag cggaagccgc aggcgcgttt ccacttcaat gggacggcgg acggtccgat
    34021 ccaggaccag ctcgatcagg aacgccagcg caaacccggc aaccacgccg ccgagggcga
    34081 tcaggccgag catctgaacc aactgccgcg attcccgaaa cgccgggctg ggcgcctgcg
    34141 ccacgccgat gttcgacatg cggccggcac tcaaagtctc gtcaaaccgc gcctgctcca
    34201 ggctggtgga gaagtggcga tacttggctt cctccagctc cttttttcgc tgcaactcga
    34261 cgatgccggg ctcgagttcg ttcaccgcct tggcttcggc ggtcaactgg tccatttgcg
    34321 aattgagcac cttgatcttg gtctgcagcg ccgcaatcga gatgcgcgcc cgttcctcca
    34381 ggaaatcgcc aaccgcctcg ccgggcttgg cggagggcgg ctgcagccgc gccagccgcg
    34441 gataatccgc ttccagcttc tgccgcgtct gttccagcgc ggcgatttgc tcgcggatgc
    34501 cccgcaccat cgcgttctct tccgtgtact gggtcagcag cccctgctcc tgtttgcgaa
    34561 acgaatccag gcgcgcgacg atgttccgat acgactccag cttttcgggc ggaacttcgg
    34621 cgtcgggcgc cgcggcggag gcttccggct ttttcggcag cagcttctgg tgctcctcga
    34681 gcaccgcctg ctgctgggcc aattcggcct gggtgttgaa cagttcctgc cggatttttg
    34741 cgagcgactc gctataggcc ttcttcatct cctcgagcgg caccacgcca atgttggtcc
    34801 tggctttgcg caagtcctgc tcggtttggg ccagttgtga gcgcaacgcg tcggtctgct
    34861 gggtcaacac atcatcaaaa ccgcccatgc tccggtggat ttgaacgtgc ttgcgaaggt
    34921 aggcctcgat gatctgcgtc agcaccggtt gcacaacctc ccggttggga ttctggaaga
    34981 caatccgcag gacgttgctg cggcggggaa cctcaacctt cagattctgc tgcagcaaga
    35041 cggcggcctg gtacttgttc gtgttggccg ggccggcccc gaggattttc cccgccccga
    35101 tgatgtccac cacttgcgtc gccaggtcca ggctttggag cacctcaacc tccgaattga
    35161 tgatgttgtc cccccgcgga tcggggttct tgatctgggg atcccccgct atcccgctgg
    35221 gcatgctgga ctcgaccacg tagcggatga gcagcttggc ttccgagaca taaatgggcg
    35281 gcgaggtgaa ataaaccacc gcggccaccg cgagtcccag cacggagaag ctgaggatct
    35341 tccacttatg ccgaaagatg atgtagtaaa tatccgccag ggttagcccg gtcgaatgtt
    35401 gcggccgttc gtgcgtttgc ataaattatc gtcgttctat tcgggcgctt cgtcaggtaa
    35461 ttcgtctttc ccggcgggcc catacgcccg gaaagttgga cggtcaggcg acatggcgat
    35521 ataacagcgc ccccagcagc cgcgacaggc tcaacggcag cgcgccaaaa acccggttgt
    35581 gccaccccac cgcctcgtca tgccccggca caaacgcgtt cttccgcaga tcgtaccggt
    35641 agtaaggaat cgggtattcc cgcgcccccc agccccgctt gaagcggagc aacccttcgt
    35701 tggccggcga agtccggccc atatacagct tccggcagcc ccgggcggca taatgccgaa
    35761 tgccctccca gatcaccaaa tcattgccgc gcagccggag cgtccgctca tcggaagccc
    35821 caaacttgta gagcgctgtt gcccccttct gaaaaaagac ggcgccggca atgtagcgcg
    35881 cgccggcgcg cgccagcacc acaatgccat ccccgtgatc caggatgcgc tcctgcacct
    35941 gcaggaagaa ccgcagcggc tgaggcggca aaccatgctt gcgccgcgtc cggcagtgca
    36001 gcgcgtaaaa ttgccggatt ccccggacgt cactccgaaa ctcaaccgtg acgccctcgt
    36061 gttgcgcctt gcgcaccgcg cgccgcgtcg ctgacttcag ggccgcaaac aactccggct
    36121 cctccccggc cagggaaagc tcgtgcccat aatactgctg cgccggcgga gccgcggcca
    36181 gaaacggctg cccgccccgc aattccaaat acttccagcg ccggctccgg ccatgcgcca
    36241 cggccgcgtc aaacagttgc cgaaaagccg ccgcatcggc cgccagcgga tcgcaataat
    36301 cggtaaaggg cagcgaaatg ccccggcgtc ccgtcagcca gctatccacc tccatcaccg
    36361 gcagcagcgc ctcgagcctg cccccggttt gctccgcaaa atactccggc gcgtagccgt
    36421 aggtctgcgc cagcaccagc gcccagccct tgcccggaaa gaaagcgtcc ggagccacgg
    36481 cggccagctt gtcattccac cgctcatccc gaatgggatt cagccgttgc accactctac
    36541 cgtgggccag caaggccccc cgggcaagta tttccaccgg cgccggcgat tcaaaaggga
    36601 agacagaccg gcccggcgcg ctcacctggc taaaactcgc gctcatcggg ccgccgtacc
    36661 cccggttgca ccgcccgccg gaatgcgggc aaagctccgc acttggtgtt tcgcagactc
    36721 tctgcgccac cttccggccg gctcccggag tcctccggaa gcttgcttta atcttttcac
    36781 gattaccgat cggcaggcga cccttcgcct gcgggccccc ggagcgtcca ccgcgagctc
    36841 caacatccgc aaacaccagg ccaattatgc ccgggccagc tccggcagcc actccgccac
    36901 cgaatcccgc ggcaggctca cctggcggcg gtccatttcc accggcgtgg gccgccccag
    36961 aatctccagc aggatttgca cccggcgccg ggcgggccag ctttgcagca ccagggcctg
    37021 cattcccata aaagcgccgc gcgtcaccgt cacctcgtcg cccggccgcg ggtccttccg
    37081 caacggcaga agctcctcgg cgccaaaaca ccgccgcaaa tcctccagca cctcggccgg
    37141 cacttgcgga atgcgcccgc cgaagctcac cagcccgcta atccccgaaa tgtggcgaat
    37201 ctgcccgata tgctcatcca ggaaacaccg cacaaaaaca tagccgggaa aaaccggttc
    37261 cgtaatccgc ttgaccaccc cgcgaatcgt cgtctgctcc gagcgcaacc tggggctgaa
    37321 aacctccaac cccaggcggt ggcgcagccc ggccgccgca atatgatcct gtttgggctt
    37381 ggttttggca caataccacc cccatccccc tccccacggc gcgcccgccg cgccaggccc
    37441 ccaccccgga gccacggcgc cagccgacac ccggtgggcg ctgaaactct gttcggcttt
    37501 aatgatcatg cctcaaccac tattttgact gataaccatt ttcccactgc gctgttgagg
    37561 ggtcaaccat ccatgccggg aaccttcacg gacagcctga ccgtagatat acggaaaacg
    37621 ctgcaaaaca cgatatcccc gacaatttca aggatatccg ccgcgtcagc cgcaaaccgt
    37681 tccccggccc gcgtcgctca agaccgcgcc aacgaaaccg atcccaaccg gaagaaagaa
    37741 gcatgacaac ggggtagcag gcgggatgcc accccgcggc tcagaaccac gtaaaccgtt
    37801 cccgcacaaa gacaatatcg gacggctgca ggtaaaacga ctcgccaccc tgtccctgca
    37861 aatgccgctt caaattcaaa tgatattgct cctcgcgctc gccctggcgc cgaattacgg
    37921 tcacgtcttt cagattcgcc ttcgcgtaat ccacccccgc atcaatcacg gcctccagga
    37981 ccgtcaccgg gcggtcaaac gcgatccgac cgggacgcgc caccgcgccc gtcacaaaca
    38041 cgaaataggc ggaggactcc aatgccacat tcacctcttt ggtcaccaac tgcggcgcat
    38101 agagtttgac cagttccttc tccaaatcgg tcgggctcat cccggcggcc ttgcactcgc
    38161 ccaccagcgg cagcgacagc ttgccgtcgc gccgaatctg ttgaatcgta ttcaggttgg
    38221 gagaccccgg aaaggtgatc cgcaccgtgt ccccctcccg cagcatcaac gactccgccg
    38281 tgggagacgc cgagggcgaa gccggagacg ccgccacggc gcccgacggc gaaatctccg
    38341 caaaattagc cgattgcttc gctcccggcc cctggcaacc cgccagcaaa accaccagca
    38401 acaggctcaa accgccaacc cacatccggc ccgcgattcc atcccaaact gactcgttaa
    38461 gctttttcat aggaagttcc accttttcag ggattgttgc acacatgtac gaccattatt
    38521 cgttttcttt aattcaccgc tcagcaggaa aaataccgcg tccccgcgaa aagacaagct
    38581 gaacctgcct ttttcctgaa cacgcccgcg cggcctccgg ccaacgaaag ctactctccc
    38641 gcatcggccg gcccgacgcg caattccact gattacggca gtttttgcga gcaattccaa
    38701 cgccaagcct ctcacgcagc cgcgcctttg cccggggctg gataacttcg agcccggaaa
    38761 ctgcgtttgg agaactatcg cgcggcgact ggcggacgga aaatcgaggc caaggcaccg
    38821 gagcggagcc agtgagccgg gcgagggagg gaaaacccga aaggcgcgaa atagcgcgat
    38881 cgccgcatcc attccgttca acttcaccca ccaaacgctt cattggggcc acgtcccgga
    38941 cggccatgcc cgccccgatg ccgatggtac gatggccctt gcgcggcctg cccaacggaa
    39001 cccctccaaa taagccagcc gcgacgccaa aagcccccaa acgctcgcct ttccttgaaa
    39061 aaccactccc gtccagcctt ggatttataa ctaactgttt ttacgcaact tatccttttc
    39121 cccaccattc cgcctcatcg tctcggcctt tccaagcccc ccttaccatt acccagatac
    39181 cgtggatata ccctgtccat aggccgtgta caaggggtgt acaagggatg cccatcgggt
    39241 gtaccagcta aaatcagttg tgtccgtcaa gttcagcaaa ttggttatca tggtttccac
    39301 tcaaggt
//
LOCUS       DAOPQG010000021        39115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1382_length_39115_cov_15.473144, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000021 DAOPQG010000000
VERSION     DAOPQG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 39115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39115
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1382_length_39115_cov_15.473144"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(536..814)
                     /locus_tag="PKI07_03840"
     CDS             complement(536..814)
                     /locus_tag="PKI07_03840"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOC50214.1"
                     /translation="MAKQVKASPKQKFSLSAPEAKSVLLVGDFTQWQQQAIRMRKGKN
                     GVWTTSVALPPGKHNYRFLVDGEWRDDPECVLRTGNPYGTHNMVREVG"
     gene            1448..7990
                     /locus_tag="PKI07_03845"
     CDS             1448..7990
                     /locus_tag="PKI07_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOC50215.1"
                     /translation="MKTYTSLIPEAEQRPAAMLGRLMLAAGALGSAGALYASTDYGPA
                     IWRPVNCAKYSTTGYGHRFIVIHSMEGYYQTGTSYLRQCGVQASCHYTVNGRKDYASD
                     AAPGEISQLVREAYYAWHATCWNRYSLGTEHEGFVGNPAWFTDEMYKASAALHKHMAE
                     KFGIAKDRNHIIAHGQKSSSAWRSWMYSQGYSSSFVNCNTHTDPGPYWNWTHFMNLIK
                     GTSSAPAAPSGLAAEKVNASQIKLTWKDNSSNESGFKIERATASGGPFTQIATVGANV
                     KTYTSTGLKALTTYWFRVRAYNSAGNSGYSNTDSAKTDGLPPAAPGSLAATTVSTSQI
                     KLTWKDNSSDETGFKIERATSAGGTYTQIATVGANVTTYANTGLSGSTTYYYRIRSYN
                     TWGNSAYTAVASSTTANVAPVLAAIPNKTVNEGSLLQFSVSATDADEVNATQTITDFE
                     SYEAETVNGTVMFRHPGNSGSTSGFLTTATNYTTVVASGGGRTSKMLKGQWTFGTSKP
                     NPWVRLTTYNTTSLPNPTVGINQRLKFDIYSSKALKVGLGIRETGTTAAIGANGGTTG
                     TIEWVGVNGSTSGTPTPTRSVAANTWTTLSFNIPFEPVTAFTGDGVLQRTTGKGVLEH
                     LALVADGGTGAYTIYLDNFVYVRDNNITYSLIGAPAGASIDPTTGRFTWTPTEAQGPG
                     TYTITVKATDAGSPPQSDTQTFTVTVNEVYDGPRLATIPNKSVNEGALLSFTAIASNS
                     NPALTLTFSLDAGAPAGASINPVSGLFTWTPSEAQGPGSYPITVRVTDNGSPAKSDAR
                     TFMVTVAEVNTAPVLAAIPPQTVDELTLLSFAASVTDADLPGDQITFSLDAGAPPGAA
                     IDPETGVFTWLPTEAQGPGVYMITVRATDDGSPALSASQGVTITVREVNTPPALAAIP
                     SQRIRPTRPLTFAAVATDPDLPANPLTFSLDPGAPAGAAIDPATGVFTWTPTPLQNNT
                     TNLIKVRVSDGWASDVKNVVVVVSDANNPPVLAALPDKTVNEKTALTFTASASDPDGD
                     SLTYSLAAGAPSGASINPATGAFSWTPTEAQGPGTYSITVRVTDNALPNLSDARTFTV
                     TVNEVNLAPALNPISNKTINEKATLTFTAVASDADLPANTKTFSLDAGAPAGASIDPV
                     TGVFTWTPTEAQGPAIYPITVRVTDNNPDAVNSKQMSDARTFTVTVNEVNEAPVLPAI
                     ATKTINEKSTLTLALAATDADLPANTLTYSLDSGAPAGASLNPATGVFTWTPTEAQGP
                     STNTITVRVTDDAPTPATDSRTFTIIVNEVNEAPVLAALANKTIAEGGTLSFTAAATD
                     ADLPANTLTFSLASAPAGATIHPSSGAFTWTPTGAQAPSTNLISLKVTDNGTPALSDT
                     KTFTVVVTSPNQPPVLNAIADKTVDELTTLTFTATASDPESAWAPITITTFENFAAPT
                     VNGTVMFRHPAFSGSTSAFVDSAATNYSKVVSNFPSGHSGSKVLQAAWTFKAGTTNPW
                     VRLATYDAANLPNPTIYFGHVLKFDMYTDKALKVGLALRETSTVAAIGANGGTSGTVE
                     LVGVPGMIGASPIPSRTIAAGAWATVSFSLPDEAVTALTGNGVLESMTGKGILDALVL
                     VPAGGLGAYKVYLDNFVVAPANTLTFSLDAGAPAGASINPVTGVFTWTPTEAQGPGVY
                     PITVRVTDNGQPPKSATRTFTVTVNDVNNAPVVAAIPNKTVNELSLVTFTASATDSDG
                     DPFTWSLDAGAPAGATINPATGVFAWTPSEAQGPGTYSITVRATDNGTPPMSGSRTFS
                     VTVNEVNTAPVLSVADQTVLDGQTLSFNLSASDADLPANTLTFSLVSGPAGATVSPAG
                     AFSWTPPAIAGIMFNNFEAGTNNRPYGFKEPRNSATTTSFLQELPNEMVVVTNAPSRS
                     GKAGRFNFAFVSTATTNWVRLTTYNAGGVPNPLVDLNHLVRFDIYSTRALKVSMGIRE
                     TGGAGPVGANGGATGNIEWIGATVRGTASVAPGGKSVSANSWQTLTYDLKGDPCEGFM
                     GNDILEGNWGVLESLAVTKGDTSASLTNTIYVDNFRVVPKTNYTVAVTVRVTDSTGLS
                     DTRSFNITVYTSPVEQAQGMLLAEPLAKANAIAPAPRILSTAMTSDGFNFSFGTETGK
                     TYDVEYAESLVNPKWKLLLTVAGDGRAVVINDPTPPISGQRFYRFRIR"
     gene            8126..11824
                     /locus_tag="PKI07_03850"
     CDS             8126..11824
                     /locus_tag="PKI07_03850"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC50216.1"
                     /translation="MLKRFAGAAILLLILVPGFKLAAAASSSTPSPVPDPLASHLNPD
                     GTLNLASGFSGTLDARGWALLADPQGGPRFVKAGGNTSPAFAAGSAKSGAASYTAGDE
                     NWDPRYVSAPGINGVVHALAVSDSSLYVGGMFDTVSGLSAANIAKWNGTEWETLGEGV
                     DGVVLAIAISGTNVYVGGAFAVAGGVSATNIACWNGESWSALGTGVDNEDGSGNVAAL
                     AVLGSNLYAGGVFTHADAVSASNIACWNGSAWSALDAGLGDPDPENIPGVSVLAVRGT
                     DLIAGGSFTTAGGVAATNIACWNGSAWSALGGGTDGTVLALAASGSNLYVGGNFLTAG
                     GVAAANIACWDGSTWSALGAGVDETVFAIALSGNNLYAGGAFMSAGSGDAANIARWNG
                     TTWSALSSGTGGSVFALAASGANLYVGGDFFSAGGLNAYNLALWNGFLWSTLGAGEGN
                     VPFGSILKIAVSGADVYVGGSFRSVGYVAANNIARWNGSFWSALGSGVDGPVSALCLV
                     GTDLYVGGSFATAGEVAAANIAKWDGGSWSALGDGVDDAVTAIAALGPDVYAGGNFAT
                     AGNLPANNIARWDGAAWSPLGEGLDGPVFALAAGADDLYAGGDFTTADGSVTANKIAR
                     WDGAAWSALDLGLDDVVHCIAIKGTNVYVGGNFTEAGGESASGVARWDGSSWFPLGDG
                     VGNADTNASVTALDFAGEALFIGGSFTLAGTAGATNVARWDGTQWTALGGGVNQPVVT
                     LAVGGTDVYFGGSFTRAGDQPSYHLAQWHDAPPPPNDNFEARIALSGSLVVTNGDNTG
                     GSKQMDEPYHSGIYGGRSVWWSWTAPTSDTVTISTASTAFDTVLAIYTGAELSALTEV
                     ASNDDDPNGGVTSRVTFSAAAGTPYQIAVDGYNGEYGNIALRIVVGVPPAITAQPQSQ
                     TVPAGANVTFSVAATGTVPLSYQWRLAGTNLPGATASSFTRTNVQLADAGNYSVEVAN
                     SAGSVTSQDAILTVNAPPLIIAQPQGQTVTEDDSVGFTVVVTGSPVLKYQWRFGGTNL
                     PGATGTSYLINHAQAGQAGDYSVVVTNAFGSATSSNATLVVLVPPAILAQPLDQTVSP
                     GSNATFSVTATGTAPLSYQWRFEGDDLPGATASVFTRTNVQPADAGNYSVMVTNLAGG
                     ILSSNAALTLLAPQPVALLSPQRLENGQFQFTVTGAVNQVFSILASTDLQAWTSLRTN
                     TLTNGSQVYIDSEATNFTHRFYRGLSAP"
     gene            complement(11916..13970)
                     /locus_tag="PKI07_03855"
     CDS             complement(11916..13970)
                     /locus_tag="PKI07_03855"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC50217.1"
                     /translation="MKTPDASRKWLLCLLLALATWLAYWPVLRCGFVDLDDPLYVTEN
                     YQVQAGLSGKGLAWAFTTGHAANWHPLTWLSHQLDAQLFGLRAWGHHLTSLLLHTVNA
                     ILLFLVLRRAAGSTNREEGEAGFEPQTALCALVAALFALHPAHVESVAWVAERKDVLS
                     GFFFMLTLGAYVRYAESRRKMGAERTGLGGAGLANEDERLTKGVGGRFHLPAIAFYLL
                     ALGFFALGLMSKPMLVTVPFVLLLVDYWPLRRWGKEEAELAARGRQGRKSAPPPAPSA
                     LRPLLLLLREKLPFFVLAAASSVATCLVQHAGGATVSMAELSLGGRAANALVAYVRYL
                     GKLVWPADLAVYYPRPEQWAAGRVWGAALVLAVITVLAMRMWARRRYVAMGWFWFLGM
                     LVPVIGLVQVGEQAMADRYTYLPYIGLFIMLAWGGEALARRSKYGTAFLGAAALGALA
                     VLLVVTRQQTRHWRTTETLFRQAIAVTRDNPLAQECLGNALLEAGDLDGAEQLGREAL
                     RLQPGFVEAELLCANVLARRGQLEEASRRFGEILRRNPRDANAHLSLAQVLSQSGKSA
                     RAVEHYREGLRRRPDHPEALNNLAWILATDPDANIRKGGEAVQFAKRACELTRYQRPL
                     LLGTLAAAYAEAGHFDAAVATAREAIARARALGQSGLAEKNQQLLERYQAHQPYHEPA
                     RP"
     gene            complement(13967..16291)
                     /locus_tag="PKI07_03860"
     CDS             complement(13967..16291)
                     /locus_tag="PKI07_03860"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC50218.1"
                     /translation="MESNQPPQLGTAEEPRGNRRRAVLMGIALVAATLVAFGPILRNG
                     FVNYDDGDYVTTNPEVLSGLSWAGVKWAFTAGHAGNWHPLTWLSHQLDVQLFKLQAWG
                     HHLTSLLLHAANAVLLFLVLRRATRSAAGGMSPAARDWLCAGVAGLFALHPLRVESVA
                     WVAERKDVLSGFFFMLTLGAYVRYAERRREQEGAGRETGGGRRGGFSALGCYLFALGF
                     FALGLMSKPMLVTVPFVLLLLDYWPLGRWRGRESGGAAEDRPGGQTEAAAAASHLTPH
                     PLSSPGGLSRLFLEKLPFFALAAASCAATVVAQRPAMQPLSNLALGARVSNALVAYAR
                     YLGKTFWPLNLATPYPHPGHWPLGLVLLGLVLLAGVTGLVLWQRRRHPFGVTGWFWFL
                     GMLVPVIGLVQVGEQSLADRYTYLPMVGVLIVVIWGVYEAGRRKAGRWRGLKPMLVCL
                     AALVLLGCVLRTRDQCRCWRGSERLYRHALAVSRNNFIAAYNLGGWLDGEGRTDEALT
                     YYRKAVEMQPRYPDPLNNIGCILAGRKQFAEAIPYFEAALRSRPDYVLAHENLAAACR
                     ELGRFREAIPHLEVVVKARPEDTGVLNSLANALAGVGRFAEAVPYYEASLRVRPEQVA
                     AHYGLANALVKLGRADEAVAHYRQALQAKPDFAQAQHDLGIALARQGRLEEAVLAMRE
                     AARLEAGNATYQINLGRLLAARQEWSEAIACYERAVQLAPGNADAQFHLGKALAAQGR
                     TEAAISHLREALRLRPDHAPARQALQALGAPPPP"
     gene            16641..18119
                     /locus_tag="PKI07_03865"
     CDS             16641..18119
                     /locus_tag="PKI07_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50219.1"
                     /translation="MTKQTLMEEASHPASPAYALRRERDFWEVTYEGRRARFPHELGA
                     QYVACLLLEPPREPIHAVVLALKARARFGPGGGGVLRGTPPVDLNEAATVQALWRRQR
                     ELERVLADRWTIEPVKAEALRELEEVTEFLRRSSWPCRHGAARRARAVAVAIKRLHAH
                     LAGASEADRRSHAVLTAFSRHLYHHLLVPSGRGGRHVRGWPTTPCPPGCFTYAPPAGI
                     VWQGGGAGPDAEGRGLRLKVSRWPLRGRWWPGRTAAADYLARFLSLASATALVACGCA
                     APRPFKGGRAVVTRKPAGVVEQTLQQGENAAQATRQNEESVKVRSYTVPAGSRLEPPP
                     APPGTAAGPEGGAAPATAFVLSAPMAVVEREESRARTELGAAQKDTARELGARLASLK
                     GIVWGGVGLFGFGLASLVWPPLRVAIGSVTTSAAMLLGGLALMVLPAMVVGNELLVLG
                     GVALAVGGWFLAHRHGRLRGLVTANGAANGTAEPPPAGGAPR"
     gene            18239..18910
                     /locus_tag="PKI07_03870"
     CDS             18239..18910
                     /locus_tag="PKI07_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50220.1"
                     /translation="MKILDIPQSGKRGLYVSQNSRNGLISRALVIPANPQTDAQLRVR
                     HFLQTVVTAWGSTLTQAQRDAWTAEAAQRRSRARLGQSGPLTGVQLFTKINCALLIIG
                     SEVVLTPPAAPVFNRLPVTGLSITNTGGVVALKLTTSGTPADGTMLWGAKPCSQGISA
                     APGVVFLGTLDSPVNNAIDITAAYTAKYGSPAAGKKVFVKVNQNINGWEDVPRQFWAI
                     VPAAS"
     gene            19090..19491
                     /locus_tag="PKI07_03875"
     CDS             19090..19491
                     /locus_tag="PKI07_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50221.1"
                     /translation="MSPWHDMVTSPPAEGQLVLARRLPGDCPPLAARWDAARARFQCG
                     PERWGLPWQFVAHWRPLAADPAWPRPRAGTSPWQDVYLCPPAPGQSVWLRRGGEDTAA
                     FRAVFDRAGAVFTLPNGWRISWYEVSRWRGG"
     gene            19590..20648
                     /locus_tag="PKI07_03880"
     CDS             19590..20648
                     /locus_tag="PKI07_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOC50222.1"
                     /translation="MLTEDNIRNALREVKYPGYSRDIVSFGLVNQVAVKDGAINVLMQ
                     LTVGRPDIAAQIKADSERVLKALPGAARVQVEVRYPGAGAAGGGPAATGPNPWEQQRR
                     VPGIRRIIAVASGKGGVGKSTVSVNLACALRQLGAKVGLLDCDIYGPTIPLMMGVRQM
                     PTLSAQDKMVPPENHGVKLMSMGFLVEGDQPVIWRGPMINKTIQQFFSEVEWGDLDVL
                     VVDLPPGTGDAQLSVCQTVPLDGGVIVTTPQEASLGVVRKGIGMFQKVNVPILGIVEN
                     MSYFTTPGGERVEIFGHGGGQAEAKRQQVPFLGEVPLFTEIREGGDRGVPAVVSGADQ
                     PAGKVFVEIARALRQQLA"
     gene            20803..21021
                     /locus_tag="PKI07_03885"
     CDS             20803..21021
                     /locus_tag="PKI07_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HOC50223.1"
                     /translation="MSDEMKERPSGDDLLKNSSLYREFQAEREEILKHKWIESEKAGH
                     DIGFERALTDWIIKHRAKWRKSQQTANR"
     gene            21159..21695
                     /gene="pyrR"
                     /locus_tag="PKI07_03890"
     CDS             21159..21695
                     /gene="pyrR"
                     /locus_tag="PKI07_03890"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOC50224.1"
                     /translation="MPDPTLILNAPTLQRALTRIAHEIAERNEAGSEVVLVGIQRGGV
                     PLAERLGALLTEIWGRAVATGRLDVSMHRDDLDRRVAPQVQPTVIPFDVTGKTVVLVD
                     DVLFSGRTVRAAMDALNDFGRPRRIQLAVLIDRGHRELPIKADFVGKNVPTARAESVR
                     VRLAETGGADEVVLEKPV"
     gene            21692..22633
                     /locus_tag="PKI07_03895"
     CDS             21692..22633
                     /locus_tag="PKI07_03895"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOC50225.1"
                     /translation="MSWNRKHLLDIESLTAAEITTVLDTALAFKAVGERAIKKVPALR
                     GKTVVNLFIEPSTRTRTSFELSEQRLSADIINFTAEASSLRKGETLKDTARNLEALNI
                     DFIVIRHSASGAPHFLARVLNASVINAGDGAHEHPTQALLDTFTIREKKGRLAGLKVT
                     ILGDILYSRVARSDIWALTKLGAQVTVCGPSTLVPRVFEQMGCRVTHNVEEAIREADV
                     INLLRIQHERQRKSMFPGLNEYSSLFGLNKERLALTKPDVMIMHPGPINRGVEIDSEI
                     ADSGRSVILEQVTNGLAVRMAVLFLVNGGKGPQEVAP"
     gene            complement(22754..24112)
                     /locus_tag="PKI07_03900"
     CDS             complement(22754..24112)
                     /locus_tag="PKI07_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOC50226.1"
                     /translation="MLNYIWLGLVVLAVIIGGCTDNLKAIADKSFEMAEFAVMRTALP
                     LVGIMALWLGIMRLAERAGLVAALARLLRPLMVWLFPEVPHDHPAMGAMLLNMAASIL
                     GLGNAATPLGLKAMRHLETLNPRPGTATNAMCTYLAINTSSIQLIPATAIAILAANKS
                     SNPTAIVGTSIIATTCAAVAAVTAAKILQRLPFYRLPPAPAPPAESAPEPAAPAPASA
                     GNEKTPIREAADLAPLRWWGMLIMSAFGLFFLYVFLRLAFPAVVGLTPPSDAAGQTVF
                     IRIVDAVSRISIPFMLSVFPLYAALRRVKVYEEFVDGAKEGFDVALRIIPYLVAILVA
                     MGMFRAAGGIDLISRALAPVMEAVGFPSPLLPMVLMRPLSGSGTLGLLAELVKQYGPD
                     SLISRTGGTIFGSTETTFYVLAVYFGSVGVKRTRYALPAGIVADTVGVIASVIVCRLV
                     FG"
     gene            complement(24220..25749)
                     /locus_tag="PKI07_03905"
     CDS             complement(24220..25749)
                     /locus_tag="PKI07_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOC50227.1"
                     /translation="MTSILNNGRLPGGSIRGRMWRRLSRGLLLFGLLTRLASAHDGPS
                     VIVDTDMASDDVRALALLLNCPYLNVLAIVTSDGVCPPDVGATNACRLLGFLKQSDVA
                     VGLGRKQTAPPPSFRTNATALDWDKLGEPRIPRTGLHEAASLIRLMAKAAPDPIVYLC
                     LGPLSNLADALEGDPGLAARIRSVLWYGTPPKVSPAGWNALRDEAAREKVIAAGLRVE
                     VVYWPDETVAPVLDDALLDEVAGLDSPAAELVVWLHSTGRGAELVRARHLRLWDDLVA
                     LRFLDPALGTLTPVAGQPNWSQWTPADPAAVRRSFCALLRAFPERATVIMAEFPVKPQ
                     DLLPDVRESAAQIISRHGLEEWKAAVLTSELHRHLGTYSIVGAKMGLRARERFNVALD
                     ELRVESHAGLKPPLSCLNDGLQTATGASLGRGTISVPTNVPPACEAIFRYGERRLRLS
                     LKPEFARRIAEDMAALEKKHGGLTPSYFQEVRIVSLKHWLNFDRNTMFDESEEVIRIS
                     P"
     gene            complement(25764..26780)
                     /locus_tag="PKI07_03910"
     CDS             complement(25764..26780)
                     /locus_tag="PKI07_03910"
                     /inference="COORDINATES: protein motif:HMM:NF027704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOC50228.1"
                     /translation="MTATPSKISPRNHAPLRRRIRGLVLVPLALGLAACVSTKVAPSK
                     ASRICAELRQPAGTQVLVAAHRGLSGRSTGAWEKYPENSLAAIADSIAKGIDIVEVDV
                     RKTRDGQLVLCHDATVDRTTDGTGAISDLTLAEIKRLRLRLGVGGTNAPVTNHQMPTL
                     EEVMRLVRGKCLVNLDKAWILVPECRAILQKTGTTRQAIFKSSYNAARCEVDFADLDP
                     PVLFMPIIFQKKGPENNLESGWAKIEPYTRRLRPCAFELVFADDDVPIVAPETIARIK
                     QAGARVWINTLWDSLAAGHTDAKSLTDPEAGWGWVVARGANIIQSDEAQRLLEYLRAR
                     RLHW"
     gene            complement(26782..28203)
                     /locus_tag="PKI07_03915"
     CDS             complement(26782..28203)
                     /locus_tag="PKI07_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC50229.1"
                     /translation="MSGAGGEAATRSWNPLRWFATGPDAPRITDPERVNQLYRRHRMS
                     VMFAITCGYGFGYTCRLPLAVMKKPLLDEGVFTAEWLGLIGSAFSIGYAVGKLVNGFL
                     ADHANVRRFFSATVLLSALINLAMLGFVSPWAWAILWGLNGWFQGAGSPCSGVSLANW
                     FSAKERGSYYGIFSSSHSLGEGLTFVFNSLLVTHLGWRAGFAGPGMFCLLVAFGILLL
                     LRDRPETMGLPPVADWRNDHPAGTDRAVPAADPRRDQLRALRMPAMWVLGLASACMYV
                     SRYAINSWGMLYLQEHRNYTLVEAGLMLGLNTGAGLAGCVVYGFISDRFFNARRPPVT
                     FIFGVLEVIALLMIFYTPPGHPVVLSAAFILFGFTLSGLLAVLGGLFAVDIAGRRAAG
                     AAMGFIGIVSYLGNALQEWVSGLLIQRGVTFATVTALNEQGQAVATQVKHYDFRSAIA
                     FWIGASVLSLVLAASLWRVKTHE"
     gene            complement(28269..29543)
                     /locus_tag="PKI07_03920"
     CDS             complement(28269..29543)
                     /locus_tag="PKI07_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC50230.1"
                     /translation="MLTDPAEIKAQYRYWQRRILFSTIVGYAMFYFVRKNLSVAMPAM
                     SRDLGIEKSDLGLFLTLHGLLYGFSKFAHGFVGDRTNARTFMALGLILSAGVNILFGF
                     SSAVVTLGLLWMLNGWVQGMGFPPCARLMTHWFPPKELATNFSIWNTSHSIGAGLVVV
                     LCGYLVGYGWRLCFFVPAGLALLCALFLLGALRDTPPSLGLPEVEGTASGPSDSPGFS
                     AAQLRQVFGNKYIWLLAMASFFVYIVRYAVLDWGPTFLTEMKHIELKHAGWMVAAFEV
                     SGVAGMLVSGWLTDRLFGGRGARLCVFSMLLTALSVLLFWKLPSRSPVICTLPLCLAG
                     FFLYGPQALVGVVVANLATKRLAATAIGLTGIFSYASTLFSGWGLGTLVQHYGWNITL
                     GSLTGVALLSMLLFAFVWPAKVHGYATDSEQT"
     gene            29811..31670
                     /locus_tag="PKI07_03925"
     CDS             29811..31670
                     /locus_tag="PKI07_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50231.1"
                     /translation="MKKRRLAPVPVSLPQDEGDELPLEFAPPPPSATASAAAVACYFN
                     GRPLPPDATPMRLLGVDYVHIPTANGGDLYLTRFGLPFQEHLQLENWRDEAWFETQCE
                     RLRGTSVVYRVPTKPVHGRSLSLVVKYCRVGEDVPLDTMTFEKFSQAEFNSPYEEFSL
                     VMELRERKVAERILTHRPLAIYIPPERLELWQTGRSAHRIEHKKAKFRDVELDIFRRY
                     VLIYQWINGESADAALEKAVPGAARRAEELKRLTLRAKAEMARNGFMVADHKPAHLIL
                     RWRRDGTLLRKKNGDYAYALIDFELLLRTPEHERETQSSRRAIYLSRQRDRFASPKGL
                     QYPAHLKPVRLLGVDYVWGHADSTHGSLWVVGQDPDLFDYFVPERWRHTPSKRLSTTN
                     ETYYTLTKDRINLVWKVSRVGEAPDPAAGSPGPGARAHPGYNSPFEEVAIALDLKAKG
                     IDAIYPRAIYMSGLKSTRSKLYVSDNRRYHSHQALTTPDGQPVLRPDHIYLTIWGFWN
                     GLDEILASRDDAYCLGISLLEACQDGIISARDAKRLLAREQKKLAAAGYRDLSPKPTH
                     YLLSLRHSGTLVRDDDGWPTLRICNFALIQSAGSAKPPGPAPGAPAGTHRKKG"
     gene            31723..33579
                     /locus_tag="PKI07_03930"
     CDS             31723..33579
                     /locus_tag="PKI07_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOC50232.1"
                     /translation="MTAILGISAFYHDSAAALVVDGDIVAAAQEERFTRKKHDPGFPA
                     NAIAYCLAEGGLPPEALDYVVFYDKPLTKFDRLLETYLAYAPAGFKSFRLAMPLWLKD
                     KLHLRRTLRQRLRAARRARLVFTDHHESHAASAFFPSPFERAAILTLDGVGEWSTATF
                     GVGEGNQLRLLRQLQFPHSLGLLYSAFTYYCGFKVNSGEYKLMGLAPYGQPIYQEAIL
                     RHLIRLQPDGSFWMDMQYFNYCAGLTMTSERFHWLFGGPPRSPESLLEQRHMDLAASI
                     QKVTEEIMLRMTREVWRQTQMKNLVLAGGVALNCVANGRLLREGPFEQLWIQPAAGDA
                     GGALGAALFVWHHLMDRPRPTDGQDRQKGSLLGPRYDNEQIRQFLATAEAPHQQYTNE
                     SELLAAVAQMMAEGKIIGWFQGRMEFGPRALGSRSIIGDARNTTMQATMNRKIKFRES
                     FRPFAPCVLRERAGDYFGLRADQESPYMLLVAPVLEKHRTALSAADRQTMAAAPDLRQ
                     RVNIVRSTVPAITHVDYSARVQTVDEKRHGRYYRLLQAFERLTGCPVIVNTSFNVRGE
                     PIVCTPQEAYRCFLATDMDALVLEDFVLRKEQMNQAVTAAERQKYLAQFELD"
     gene            33623..34039
                     /locus_tag="PKI07_03935"
     CDS             33623..34039
                     /locus_tag="PKI07_03935"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HOC50233.1"
                     /translation="MTWSDIPFQPPAKALRQFGAAWLIFFLALGAWQYFARQRPVLGA
                     ILMGLALVIGLLGLLRPAAVRWLFVGWMVLAFPIGWAISQLMLAVLFYGLLTPVALFF
                     RWRGRDLLQRKAAPSATTFWLSKTTPSDVRNYFRQH"
     gene            34068..34268
                     /locus_tag="PKI07_03940"
     CDS             34068..34268
                     /locus_tag="PKI07_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOC50234.1"
                     /translation="MQKQPQNEFEKAAAQPPRGGLIVELVEFLRQNKKWWLLPILILS
                     LLLGLLIVLSGSGLAPFIYTLF"
     gene            34380..35588
                     /locus_tag="PKI07_03945"
     CDS             34380..35588
                     /locus_tag="PKI07_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC50235.1"
                     /translation="MKIGLQRAVDRYAGVPACAALSLLHRLGPKPVAARPPRRILVIL
                     LSEMGSLVLTQPMFAHLKARYPDASIHALLFARNRAVLDLLAVLPGERILTLDDRSLW
                     RLAAGSLKILRTLRALQFDVVIDCELFARVSSLLSGLSGAPLRVGFHRHTQEGLYRGS
                     FINRPVLYNPYRHLSRQFLTLAAAIESETMPPGKDAPDAPCEPPPRLAFPPEELRQVA
                     AQLHADFPALPGRRLVLVYPGGGLLPIRAWPREHYFQLCAALLREGHAVGIIGLPDDK
                     SLGQAIVAHCRSPNCVDLTGYTRSLRHLLALFHHAALLITNDGGPGQFAALTPLPAII
                     LFGPETPRLYGPLAPNAFCFHLPLPCSPCLTAYNHRASPCDGDNQCLKRITPEQVLAQ
                     ARAMLRTAPG"
     gene            complement(35611..37167)
                     /locus_tag="PKI07_03950"
     CDS             complement(35611..37167)
                     /locus_tag="PKI07_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC50236.1"
                     /translation="MTDSKPVFSGPPTPEEFQRRMAEFMQQHFAGARAFPGAAAAAAG
                     APEGGEDKTRAAAFEFGYKPREVKAYLDRFVIRQDEAKKVLSVALCDHYHHVRLALAG
                     KESPNYAKQNIILIGPTGVGKTFLIRCAADLIGVPFAKADATKFSETGYVGGDVEDMV
                     RDLLRLAGGDVTRAQYGIIYIDEIDKIAAASHASGRDVSGRGVQTNLLKLMEETEVPA
                     RSPSDIAGQIQAMMDFTQRGRPAPATINTKHILFIVSGAFGGLEQIIQRRLREATIGF
                     AARERAEPSPEAVLEHSQTRDFIEFGFEPEFIGRLPVRVVCHALQVDDLFQILKSSEG
                     SIIRQYEQDFAAYGIEVLFDDPGLRRIAERAAEEQTGARGLMTVCERVFRNIKFELPS
                     TPVKRFVVTRELVDDPAGELRKILSDPAREERMVMRQLVDEFARRFHAAHGIRLRFTE
                     AAADRLVREAAEQGQPVRDLCAQRFKDYQFGLKLIVQNSGPRDFVIDADAVAAPDKVL
                     SDWVVASYRK"
     gene            complement(37499..38044)
                     /locus_tag="PKI07_03955"
     CDS             complement(37499..38044)
                     /locus_tag="PKI07_03955"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC50237.1"
                     /translation="MCVALLKLRQTGTIVPSQRFLISQMIAPVPESYSGRIIELGAGN
                     GALTLRLAARCPQARILACEINPTLARETRDNLDEAGLGDQVEVASDSAEHLLSEISR
                     RGTGPVDYVLSGIPLGNLDGERTQALIDAICGVLRPGGLYVQFQYSLLDRRRIRSCFR
                     SLRTSLVCLNIPPAVVYYAQK"
     gene            complement(38546..>39115)
                     /locus_tag="PKI07_03960"
     CDS             complement(38546..>39115)
                     /locus_tag="PKI07_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50238.1"
                     /translation="LEIVNDAEDGVRLRLNVGEAHEDIMVFGQPPCSAGRMKPRRAYY
                     LGLLGPSQNGQSDITDLYTARFGRPAPGQKVFIAVVQTRNGWKDQSRVHNAIVPPPPM
                     LKSQQAAETAKVKKSKPSAAPASTAARKAVPSPSFQAMYKGSTSEARQENMENKHEPP
                     LSLLCTPVVHGLMGALGWLGRVGRVWARA"
BASE COUNT         7101 a        13066 c        12484 g         6464 t
ORIGIN      
        1 ccgaggtaat gcgggaaggc tggcgggcgg gaggtcttcg cgcaagggca attttaggaa
       61 gttcgcggtg gcgggccggt gcggagcgcc gcgtgagggc acgcggccta caggggacgg
      121 gagggtgcgg gggggcctgt gggttgtcgg gagtgcggtg ctgcgagagg gcatggggac
      181 tgtcgcggcg ttctttcatt tcctgcaggt cgcgtgaggg cacgcggcgc tcgggggccg
      241 ttcgtccggc ggctgagaaa agcacctgcg cgcccgcctg gattgtctgt gggaaagcct
      301 gcccgccctg gagggcgatc tcgcctccgc tgttcccaat ggcccaagcc ctgggcggtc
      361 cggccagaca cctgctccgc agggcttaac cgccgcccaa tccgcagctt cagcctcccc
      421 ggcccatgat ctacaatagc aaagcccccg ggatcgaacc ggattgcgcc gccaccgccg
      481 gcggttcaaa gagcgccaat ctccccgggg ctcaggccgt tggcccgggt gggtgtcagc
      541 cgacttcgcg caccatgttg tgggtgccgt agggattgcc ggtgcgcagc acgcactccg
      601 ggtcatcccg ccactcgccg tccacgagga agcggtagtt atgctttccg ggcggcaacg
      661 ccaccgaggt tgtccaaact ccgttcttgc ccttgcgcat gcggatggct tgttgctgcc
      721 actgggtgaa gtcacccacc agtaatacgc tcttggcctc cggggcggag aggctgaact
      781 tctgctttgg ggaagcctta acttgctttg ccataaacca ttatcctttc acaaactcgg
      841 gttgttccat ccggccgccg tcgccgtcat tggcgccgcc tgccttcatc cggccgggcg
      901 ctcgcgagtt ccaccagcgc ctgacccgtc gcgactttaa atccgctccc ggtaccttgg
      961 tctcaaccgg gccgcgcgtc aagcacaaag cgaaggggcg gttgccgcgc gggcttcgct
     1021 ggaacgatgc ggttgggccg tccaactgtg tgcccgcgct ttaagagagc cgggatgggc
     1081 ctggccgctc cagccgaagc gattcttgtt taacccgcct gtgtgccgcg aaaaacaacg
     1141 tgaaagagct tgaccttttg gcgcgtaaac attaatttcc cttcttagtg ctgccgatta
     1201 ccagcaaggt taaggcatgg tcggtaattc gccgccgggc gaattttact cccggcgaca
     1261 ggtggggggg ggttgttgtc tccattttcg gacaagccct tcagccttcc gcgcgttccc
     1321 agcccccgtg cgggcggccc cgcgcggaac gccatgggca ctctttccgc agtccgaatc
     1381 cccggccggc caatccgcga accgcaaaaa ccgaatccag cgaacactcc tgaaacaagc
     1441 aaaaggcatg aaaacctaca cctccctgat cccggaagca gagcaacgcc cggcagctat
     1501 gctggggcga ctgatgttgg cagcgggcgc gctgggcagc gcgggcgcgc tttacgccag
     1561 cacggactat ggcccggcga tctggcgtcc ggtcaactgc gccaaataca gcaccacggg
     1621 ctatggccat cggtttatcg tcatccattc catggagggc tattatcaaa ccggcacttc
     1681 ttatctccgg caatgcgggg tgcaggcgag ttgtcattat acggtcaatg gccggaagga
     1741 ctacgcctcg gatgcggcgc cgggcgagat ttcccaattg gtccgcgagg cctattatgc
     1801 gtggcatgcg acctgttgga atcgctattc gctgggcacc gagcacgagg gatttgtcgg
     1861 gaacccggcc tggttcacgg atgagatgta taaggcctcg gcggcgctgc acaagcacat
     1921 ggcggagaag ttcggcattg ccaaggaccg caaccacata atcgcccacg ggcagaaatc
     1981 cagctcggcg tggcgctcct ggatgtacag ccaggggtac agctccagtt tcgtcaactg
     2041 caacacccac accgaccccg gcccgtactg gaactggact cacttcatga acttgatcaa
     2101 gggcacttcc tcggcgccgg cggccccgag cggcctggcg gcggaaaaag tgaatgcctc
     2161 gcagatcaag ctgacgtgga aggataattc gagcaacgaa agcggcttta agatcgagcg
     2221 ggccaccgct tccggcgggc ctttcaccca gatagccacg gtgggcgcca acgtaaagac
     2281 ttataccagc accggcctga aggcgctgac cacctactgg ttccgggtgc gggcctataa
     2341 ttcggccggc aattcgggct actcgaacac ggacagcgcc aagaccgacg gcctgccgcc
     2401 ggcggcgccg ggcagcctgg cggcgacgac ggtttcgacc agccagatta agctgacatg
     2461 gaaagacaat tcgagcgacg agaccgggtt caagattgaa cgggcaactt ccgccggggg
     2521 gacgtacacg caaatagcca cggtgggcgc caacgtgacg acctacgcca acaccggcct
     2581 gagcggttca accacctact attatcgcat ccgcagctat aatacatggg gcaattccgc
     2641 ctacaccgcc gtggccagct ccaccacggc gaacgtggcg ccggtcctgg ccgccatccc
     2701 caataagacc gtgaacgaag ggtcactgct gcagttcagt gtctccgcaa ccgacgccga
     2761 tgaagtgaac gcgacccaga ccattacgga ttttgaaagt tatgaggcgg agactgtgaa
     2821 cgggacggtg atgttccgcc acccgggcaa ctccggctcg accagcggtt ttttgaccac
     2881 cgcgactaat tatacgacgg tagtggcctc gggaggcggg cgcaccagca agatgctcaa
     2941 gggacagtgg acattcggaa ccagcaaacc caatccctgg gtgcggttga ccacctacaa
     3001 cacgacgtcg ctccccaacc cgacggtggg catcaatcag cggcttaagt tcgacattta
     3061 ctccagcaag gcgctgaaag tggggctggg catccgggaa acgggaacca cggctgccat
     3121 cggcgccaac ggcggaacca caggcaccat tgaatgggtg ggcgtgaatg gcagcaccag
     3181 cggaacgccc acgccgacgc gatcggtggc ggctaatacg tggaccacgc tcagtttcaa
     3241 cattcccttt gagccggtga ccgctttcac gggcgacggg gtcctccaac ggactaccgg
     3301 caaaggcgtg ctggagcatc ttgcgctggt ggccgacgga ggcacgggcg cctataccat
     3361 ctatctcgat aacttcgttt acgtgcgtga taacaacatc acgtacagcc tgatcggcgc
     3421 gcccgccggg gcgagcattg acccgacgac cgggcggttc acctggacgc cgaccgaggc
     3481 gcaagggccg ggcacctaca ccattaccgt taaggccacg gacgcgggct ctccgcccca
     3541 gtccgatacg cagaccttta cggtcaccgt caacgaggtt tacgacgggc cgcgcctggc
     3601 gacgatcccg aacaaatcag ttaatgaagg cgcgctgttg agtttcacgg cgatcgcgtc
     3661 taattccaac ccggctttga cgctgacctt cagcctggac gcgggcgcgc ccgcgggagc
     3721 cagcattaac ccggtcagcg gattgttcac ctggacgccc tccgaggcgc aggggcccgg
     3781 cagctatccg atcacggtgc gcgtgacgga taacggctcg ccagcgaaga gcgatgcccg
     3841 gacttttatg gtgacggttg ccgaggtcaa cacggcgccg gtgctggcgg cgatccctcc
     3901 ccagacggtt gatgaactga ccttgttgag ctttgccgcc agcgtcacgg atgccgacct
     3961 tcccggcgac cagattacct tcagcctgga tgccggcgct ccgcctggag cggccattga
     4021 tccggagacg ggggtcttca cctggcttcc gacggaagcc caggggccgg gcgtttatat
     4081 gatcaccgtg cgggctactg acgatggctc gcccgctctg agcgccagcc agggggtcac
     4141 gatcacggtc cgcgaagtca acacgccgcc cgcgttggcc gcgataccca gccagcgcat
     4201 ccgcccgacc cggcctttga cttttgcggc cgtggccacc gatccggacc tgcccgccaa
     4261 cccgctgacc ttcagcctcg atccgggggc ccccgcggga gcggcgattg atccggccac
     4321 gggggtcttt acctggacac cgacgcccct gcaaaataat accaccaatc tcatcaaggt
     4381 ccgcgtcagc gacggctggg cgagcgacgt gaagaacgtc gtggtggtgg tgagcgacgc
     4441 caataatccg ccggtgctgg cggccctgcc cgacaagacc gtcaatgaga agacggcgct
     4501 caccttcacg gcttcggcaa gcgatccgga tggggacagc ttgacctaca gtctcgcggc
     4561 gggagcgccc agcggggcga gcatcaatcc ggcgaccggc gcgttctcct ggacgccgac
     4621 cgaggcgcaa gggccgggca cctattcgat caccgtgcgg gtgaccgata atgcactgcc
     4681 gaatttgagc gatgccagga cctttacggt gacggtgaac gaggtgaatc ttgcgccggc
     4741 gttaaacccg atctccaaca agaccatcaa tgaaaaggcc acgctgacct ttaccgcggt
     4801 agcttcggat gcggacctgc cggccaatac caagactttc agcctggacg cgggcgcccc
     4861 ggcgggcgcc agcattgatc cagtcaccgg cgtctttacc tggactccca ccgaagccca
     4921 ggggccggcg atctatccca tcacggtccg ggtgacggat aacaacccgg atgccgtcaa
     4981 cagcaagcag atgagcgacg ccaggacgtt caccgtgacg gtaaacgagg tgaacgaggc
     5041 gcccgttctg ccggcgatcg ccaccaagac catcaacgag aagagcacct tgaccttggc
     5101 gctggcggcg accgacgccg atctgcccgc caacacgtta acttacagcc tggacagcgg
     5161 agcccccgcc ggggccagcc tgaacccggc cacgggcgtt ttcacctgga cgccgaccga
     5221 ggcccagggg cccagcacga atacgatcac ggtgcgcgtg acggacgatg cccccacgcc
     5281 cgcgaccgac agccggactt ttaccattat cgtcaatgag gtaaacgaag cgccggtgct
     5341 ggcggccctg gccaacaaga ctattgccga gggcggcacg ctgagcttca cggccgcggc
     5401 gaccgatgcc gacttgccgg ccaataccct gaccttcagc ctggccagcg cgccggctgg
     5461 cgccaccatt caccccagca gcggcgcgtt cacctggacg ccgaccgggg cgcaggctcc
     5521 gagcacgaac cttatttccc tcaaagtgac ggacaatggc acgccggccc tgagtgatac
     5581 caaaactttt acggtggtcg tgacctctcc caatcagccg cccgttctga acgcgattgc
     5641 ggacaagacg gtggatgaat tgacgacgct gacgttcacc gccacggcca gcgatccgga
     5701 gtccgcctgg gcaccaatca ccatcaccac ctttgagaac ttcgccgcgc ccacggtgaa
     5761 tgggaccgtg atgttccggc acccggcctt ctcgggcagc accagcgctt ttgtggactc
     5821 ggcggcgacc aattattcga aggtggtcag caacttcccg agcgggcatt ccggcagcaa
     5881 ggtgctgcag gcggcgtgga cattcaaagc cggcacgacc aacccgtggg tgcgcttggc
     5941 cacctacgat gcggcgaatc taccgaatcc gacgatctac tttggccacg tgctcaaatt
     6001 cgatatgtac accgacaagg cgctgaaagt gggcctggcg ctgcgcgaga ccagcaccgt
     6061 ggccgccatt ggggccaacg gcggcaccag cggcaccgtg gagctggtgg gcgtgcccgg
     6121 catgatcggg gcctcgccca tccccagccg caccattgcc gccggcgcat gggccacggt
     6181 cagcttcagc cttccggatg aagccgtgac cgcgctcacg ggcaatggcg tcctcgaatc
     6241 catgaccggc aaggggattc tggatgcgtt ggtcctagtc ccggcgggcg gcctgggagc
     6301 ctataaggtt tacctggata acttcgtcgt cgcgcctgcc aataccctga cgttcagcct
     6361 cgacgcgggg gcgccggctg gagccagcat caacccggtc accggtgtgt tcacgtggac
     6421 tccgaccgaa gcgcaggggc ccggtgttta tccgattacc gtgcgggtga ccgataatgg
     6481 gcagccgccc aagagcgcca cccgcacctt caccgtcact gtgaatgatg taaataacgc
     6541 gccggtcgtg gctgccatcc ccaataagac cgtcaatgag ctgtccttgg tgacgttcac
     6601 cgcctcggcg accgactccg atggagaccc cttcacctgg agcctggatg ccggagcgcc
     6661 cgccggcgcg acgattaacc cggcgaccgg ggtgtttgcc tggacgcctt ccgaggcgca
     6721 gggccccggc acctacagca tcacggtgcg cgccaccgac aacggaactc cccccatgag
     6781 cggttcccgc accttcagcg tcacggtgaa cgaagtcaat accgcgcccg tgctgtccgt
     6841 ggccgaccag accgtccttg atggtcagac gctgagcttc aatctgtcgg cttccgatgc
     6901 ggacttgccg gccaatacgc tgacgttcag cctggtcagc ggccctgccg gggcgacggt
     6961 gtccccggcc ggcgcgttca gttggacgcc tcccgccatt gccgggatta tgtttaacaa
     7021 ctttgaagcc ggcacgaaca accggcctta tggcttcaaa gagccgcgca actccgcgac
     7081 caccacgtcc ttcctgcagg agttgccgaa tgagatggtg gtcgtcacca atgcgccaag
     7141 ccgcagcggc aaggcgggca ggttcaactt cgccttcgtc agcaccgcga ccaccaactg
     7201 ggtgcgcctg accacgtata acgccggcgg cgtgcccaac ccgctggtgg atttgaatca
     7261 tctcgtgcgt tttgacatct actcaacgcg cgcgctgaag gtgtctatgg gcattcgtga
     7321 gaccggcggc gccggccccg tgggcgccaa cggcggcgcc accggcaata ttgaatggat
     7381 cggggccacg gtgcgcggca ccgccagcgt cgcaccgggc ggcaagagcg ttagcgcgaa
     7441 ttcctggcaa acgctcacct atgatctgaa aggagacccg tgcgaaggct tcatgggcaa
     7501 cgacattctt gagggcaact ggggagtgct ggaaagcctg gccgtgacga agggcgatac
     7561 cagcgccagc ctcaccaata cgatttatgt ggacaacttc cgcgtggtgc ccaagaccaa
     7621 ctataccgtc gccgtcaccg tgcgggtgac tgactcgacc ggtttgagcg acacccggag
     7681 cttcaacata accgtttata cctcacccgt ggagcaggcc cagggaatgc tgcttgcgga
     7741 accactggcc aaagcgaacg caatcgcgcc cgcgcccagg attctgagca ccgcaatgac
     7801 cagcgacggc ttcaacttca gcttcgggac cgaaaccgga aagacctacg acgtggaata
     7861 tgcggaatcg ctggtgaatc cgaagtggaa gctgctgctg acagtagctg gcgatggcag
     7921 ggcggtggtt attaacgacc ccacgccgcc gatctccggg caacggtttt accgcttccg
     7981 gattcgttaa gccgcggcaa agtattgcgc caacgagccg tcctttggca caattcggtc
     8041 ggtaatgccg gcggcggttt gttggcgcaa accgccgccg tttgcagatg caaccttgtc
     8101 aggcccaaac caatctattt aagctatgtt gaaacgcttt gccggtgcgg caattttact
     8161 cctgatcctg gttcccggtt ttaagctggc ggcggccgcg tcgtcgagta cgccgagtcc
     8221 cgtcccggac ccactggcct cgcatcttaa cccggatggc acgctgaacc tggccagcgg
     8281 cttctccggc acgctcgatg cccggggctg ggccttgctc gccgaccctc agggcggccc
     8341 gcgattcgtc aaggcgggtg gcaacacttc tccggcgttc gcagccggtt ccgccaaaag
     8401 cggcgccgct tcctatacgg ccggggatga gaattgggac ccgcgctatg tgtcggcgcc
     8461 gggaatcaat ggggttgtgc atgccctggc cgtcagcgac tcaagcctct atgtcggcgg
     8521 catgttcgac accgtcagcg gcttgagcgc cgccaatatc gccaaatgga atgggaccga
     8581 atgggaaacc ctgggcgaag gggtggacgg cgtcgtgctg gccatcgcga tttccggaac
     8641 caacgtctat gtgggcggcg cctttgcggt cgccggcggc gtcagcgcca ccaacatcgc
     8701 ctgctggaac ggcgaaagct ggtcagcgct gggaacggga gtggacaacg aggacggttc
     8761 cggcaacgtg gcggccctgg ccgtgctggg ctccaacctc tacgcgggcg gcgtcttcac
     8821 tcacgccgac gcggtgagcg ccagcaatat cgcctgctgg aacggctcgg catggtcggc
     8881 gctggatgcg ggactcggcg acccggaccc tgaaaatatc ccgggcgttt ctgtgctggc
     8941 cgtcagggga accgacctga ttgccggcgg gtccttcacc accgctggcg gcgtcgcggc
     9001 caccaatatc gcctgttgga atggcagcgc ctggtctgcc cttggcgggg gtacggacgg
     9061 cacggtgctg gccctggccg ccagcggctc caacctctat gtcggcggca atttcctcac
     9121 ggccggcggc gtggccgccg cgaacattgc ctgctgggac ggcagcacct ggtccgccct
     9181 tggcgccggc gtggatgaaa ccgtcttcgc catagccctt agcggcaata acctctatgc
     9241 gggcggggcc ttcatgtccg ccggctccgg cgatgccgcc aacatcgccc gctggaacgg
     9301 aaccacctgg tcggcgctga gttcgggcac tggcggcagc gtctttgccc tcgccgccag
     9361 cggcgccaac ctctacgtcg gcggtgattt ttttagtgcg ggcggcctga acgcctacaa
     9421 tctggccctg tggaatggct ttctgtggtc cacgctcggc gccggcgagg gcaatgtgcc
     9481 gttcggctcg atcctgaaga ttgcagtcag cggggcggac gtttatgtag gcggctcttt
     9541 ccgctcggtc ggttatgtgg ccgccaacaa cattgcccgc tggaacggct ccttctggtc
     9601 ggccctcggc agcggtgtgg atggcccggt ttccgccctg tgccttgtcg gcactgacct
     9661 ttacgtcggc ggctccttcg ccaccgccgg cgaagttgcg gcggccaaca tcgccaaatg
     9721 ggacggcggc agttggtcgg ccctgggaga tggggtggac gatgcggtga cggccatcgc
     9781 ggctttgggc cctgatgtct atgccggggg caattttgcc accgccggaa atctgcccgc
     9841 caacaacatc gcccgctggg atggcgcggc ctggtctccg ttaggcgaag ggctggatgg
     9901 cccggtgttc gcgctggcag ccggcgcgga cgacctgtat gccggcggcg atttcaccac
     9961 ggccgacggc tccgttacgg ccaacaaaat cgcgcgctgg gacggcgcgg cctggtcggc
    10021 gctggatctg ggcctggatg acgtggtgca ctgcatcgcg attaaaggga ctaacgtcta
    10081 tgtcgggggt aatttcaccg aagccggggg tgaaagcgcg tccggggtgg cccgctggga
    10141 cggctcgagc tggttccccc tgggcgacgg cgtgggcaac gccgacacca atgcctcggt
    10201 gacggcgctc gacttcgccg gcgaagccct cttcatcggc ggctccttca ccctggccgg
    10261 gacggctggc gccaccaacg tcgcccgctg ggatggaacc caatggacgg cgctgggcgg
    10321 cggggtcaat cagccagtgg tcaccctggc tgtggggggg acggatgtct attttggcgg
    10381 aagtttcacc cgcgccggcg atcagccctc ctaccacctg gcccaatggc atgacgcccc
    10441 gccgcccccc aacgacaatt tcgaggcgcg cattgctttg tcaggttccc tggtggtgac
    10501 caatggcgat aataccggcg gcagcaagca aatggatgaa ccctaccatt ccggcatcta
    10561 cggcgggcga tccgtctggt ggtcctggac cgcccccacc agcgacaccg ttaccatttc
    10621 gaccgccagc accgcctttg acaccgtgct ggccatctac accggcgccg aactgtccgc
    10681 tctgaccgaa gtcgccagca acgacgatga tcccaacggc ggcgtcacca gccgggttac
    10741 cttctccgcc gccgccggca ccccctacca gattgcggtg gacggctaca acggcgagta
    10801 tggcaatatc gctttgcgca tcgtggtcgg cgtcccgccg gccatcacag cccagccgca
    10861 aagccagacc gtccccgccg gcgccaatgt caccttctcc gtcgcggcca cgggcacggt
    10921 gcccctgagc taccaatggc gcctggccgg caccaacctg ccgggagcca ccgccagcag
    10981 cttcacccgg accaatgtcc aactcgccga cgcgggcaac tattccgtcg aggtcgccaa
    11041 cagcgcgggc tcggtcacca gccaggatgc catcctgacc gtaaacgcgc cgccgctgat
    11101 catcgcccag ccgcaaggcc agaccgtgac cgaagacgac agcgtcggct tcaccgtggt
    11161 ggtcaccgga tccccggtgc tgaaatacca gtggcgcttc ggcggcacca atctgccggg
    11221 agccaccggg accagctacc taatcaacca tgcccaggcc ggccaggccg gggactactc
    11281 ggtggtggtg accaacgcct tcggctccgc caccagctcc aacgccaccc tggtggtgct
    11341 tgtgcctccc gccattctgg cccagccgct tgaccaaacc gtctcccccg gctccaacgc
    11401 gacattctcc gtcaccgcca ccggcaccgc gccgctcagc taccagtggc gttttgaggg
    11461 cgatgacctg ccgggggcca cggccagcgt ctttacccgc accaacgtcc agcccgccga
    11521 cgcgggcaac tactcggtca tggtcaccaa cctcgccggc ggcatcctga gctcaaacgc
    11581 ggcgctcacc ctccttgcgc ctcagccggt ggcgctcctg tcgccccagc gcctggaaaa
    11641 cggccagttc caattcacgg taactggcgc cgtcaaccag gtcttcagca tcctcgcctc
    11701 gactgatctc caggcctgga cttccctccg caccaacacg ctcaccaacg gcagccaggt
    11761 gtatattgat tccgaggcaa ccaacttcac tcatcgcttc tatcgcggcc tgtccgcgcc
    11821 gtagagaaag ctcgcgaaca ttgcccgatc cgataacggc tatcatcctc tgggggttgc
    11881 tttgggatga tggcgctttc ccaggggagg agcggctatg gcctggccgg ttcgtgatac
    11941 ggctgatgcg cctgatagcg ttccagtaat tgctggttct tttccgcaag gccgctctgt
    12001 cccaatgccc gcgcacgggc gatcgcctcc cgggcggtcg cgaccgccgc gtcgaaatgc
    12061 ccggcttccg cataagcggc cgccagggtg cccagcagaa ggggacgctg gtagcgcgtc
    12121 aactcgcagg cgcgtttcgc gaattgaacc gcctccccgc ccttgcgaat gttggcgtcc
    12181 gggtccgtcg ccaggatcca cgccaaatta ttaagggctt cgggatgatc gggcctccgg
    12241 cgcaaacctt cgcggtaatg ttcgaccgcc cgggcgcttt tgccgctctg actgagcacc
    12301 tgcgccaaac ttaaatgggc gttcgcatcc cgcgggtttc ggcgcaggat ttccccgaag
    12361 cggcgggaag cttcctccag ttgcccgcgc cgcgccagga cgttggcgca caggagttcc
    12421 gcttctacaa atcccggctg caaccgcaga gcctcccgac ccagttgttc ggctccgtcc
    12481 aggtcgccag cttccagcaa agcgttcccc aggcactcct gcgccagtgg gttgtcccgg
    12541 gtcaccgcaa tcgcctggcg aaagagcgtc tccgtcgttc gccagtgccg cgtttgctgc
    12601 cgggtcacca ccagcaaaac ggccagcgcc ccaagggcgg ccgcacctaa aaacgccgtc
    12661 ccatacttgc ttcggcgggc aagggcttcg ccaccccagg ccagcatgat gaacaagcca
    12721 atgtacggca ggtaagtgta ccgatccgcc atcgcctgct ccccgacctg caccagccca
    12781 atgaccggca ccagcatgcc caaaaaccaa aaccagccca ttgccacata gcgccgccgc
    12841 gcccacatcc gcatcgccag cacggtgatg accgccaaca ccagcgctgc gccccaaacc
    12901 cgtcccgcgg cccattgctc cgggcgcggg tagtaaaccg ccaggtccgc cggccagacc
    12961 agcttgccca ggtagcgaac ataagccacc agcgcattgg ccgcccgccc gcccagcgac
    13021 aactccgcca tcgaaaccgt cgcgcctccc gcatgctgca ccaggcacgt cgcgacgctt
    13081 gaagccgccg ccaggacgaa gaaaggcagc ttttcccgaa ggagaaggag tagcgggcgc
    13141 agcgcggagg gcgcgggggg cggggcggat ttcctgccct gcctgcctct cgccgccaac
    13201 tccgcttctt cctttcccca ccgccgcaac ggccaataat ccaccaacag cagcacgaag
    13261 ggcaccgtca ccagcatggg cttgctcatc agccccaggg caaagaaacc cagcgcgagc
    13321 aggtagaagg cgatggcggg gagatggaag cgccctccta cgcctttcgt caaccgctca
    13381 tcttcattcg ccaaacctgc tcctcccagc cccgttcttt ccgctcccat cttccgccgg
    13441 ctttccgcat atcgcacata cgcccccagc gtcagcataa agaaaaaccc gctcagcacg
    13501 tccttgcgtt ccgccaccca ggcaacggat tcgacatgcg ccggatgcaa ggcgaacagc
    13561 gcggccacca gggcgcaaag cgcggtttgc ggttcaaacc cggcctcccc ctcttcacgg
    13621 ttcgtgcttc ccgcggcccg ccgcaacacc agaaacagca gtatcgcgtt aaccgtgtgc
    13681 agcagcaggc tcgtcagatg atgtccccac gcccgcaacc caaaaagctg cgcgtccaac
    13741 tggtgcgaga gccacgtcag cggatgccag ttggccgcgt ggcccgtggt aaacgcccag
    13801 gccagccctt tcccgctcag cccggcctgg acttgatagt tttcggtgac atagagcggg
    13861 tcatcaaggt ccacaaaccc gcagcgcagg accggccaat acgccagcca ggttgccagc
    13921 gccagcagca ggcagagcag ccatttccgg ctcgcgtccg gggttttcat ggcgggggcg
    13981 gcgcgcccag tgcctggagc gcctgccggg ccggggcgtg atccggccgc agccgcagcg
    14041 cctcgcgcaa atggctgatc gccgcttcgg tccggccctg ggccgccagc gctttgccca
    14101 gatgaaactg tgcgtcggca ttgccggggg cgagttgcac ggcccgttca tagcaggcaa
    14161 tggcttcact ccattcctgc cgcgccgcca gcagccgtcc caaattgatt tgataggtgg
    14221 cgttgccggc ctcgaggcgc gccgcttccc gcatcgccag caccgcttcc tcgagccgtc
    14281 cctgccgggc cagcgcaatg cccaggtcat gctgcgcctg cgcaaaatcc ggctttgcct
    14341 gcaacgcctg ccggtaatgg gccacggctt cgtcggcccg ccccagtttg accagcgcat
    14401 tggccagccc ataatgcgcc gcaacttgct cgggccggac ccgcaaagaa gcctcgtaat
    14461 acggcaccgc ctcggcaaac cgccccaccc cggccagcgc gttggccagg ctgttcaaaa
    14521 cgcccgtgtc ctccggccgg gctttgacga ccacctccag gtgcgggatg gcctcgcgga
    14581 acctccccaa ctcgcggcag gcggcggcca ggttctcatg cgccagcaca taatccggcc
    14641 gggaacgcag cgccgcctcg aaatacggga tggcctcggc aaattgcttc cgcccggcca
    14701 ggatgcaccc gatgttgttc aacggatccg gatagcgcgg ctgcatttcc accgccttgc
    14761 ggtagtacgt caacgcctcg tccgtgcgcc cctcaccgtc cagccacccg cccaggttgt
    14821 aggcggctat aaaattgttg cggctgaccg ccagggcatg gcggtaaagg cgctcgctgc
    14881 cgcgccagca acgacactgg tcgcgcgtcc gcagcacaca ccccaacagc acgagcgccg
    14941 ccaggcaaac cagcatcggt ttaagccccc tccaccgccc cgctttccgc cgtcccgcct
    15001 cgtaaacgcc ccagatcact acgatgagaa cgcccaccat tggcaggtag gtgtagcgat
    15061 cggccaggga ttgctcgccc acctgcacca gcccaatcac cggcaccaac atgcccaaaa
    15121 accagaacca gccggtcacc ccaaaaggat gccgccgccg ctgccaaagc accaatcccg
    15181 tcacgcccgc cagcaaaacc aatcccagca aaaccaaccc cagcggccaa tgccccggat
    15241 gcggatacgg cgtcgccagg ttcagcggcc aaaaagtttt ccccaggtaa cgcgcatacg
    15301 ccaccagcgc attgctcacc cgcgccccca acgccaggtt cgacagcggc tgcatcgccg
    15361 gccgctgcgc caccaccgtc gccgcgcacg aagccgccgc cagggcaaag aagggcagct
    15421 tttccaggaa aagccgggaa agcccgccgg gggaagaaag cggatgcggc gtgaggtgag
    15481 acgccgccgc cgccgcctcg gtctgcccgc cgggccggtc ttccgccgcc ccgccgcttt
    15541 cgcgcccccg ccaccgcccc agcggccaat aatccagcag caacagcacg aagggcaccg
    15601 tcaccagcat cggcttgctc atcagcccca gggcaaagaa acccagggcg aaaagatagc
    15661 aacccaacgc ggaaaaaccc ccgcgccgcc cgccgcccgt ttcccgcccc gccccttcct
    15721 gttcccgccg tcgttcggca taccgcacat acgcccccag cgtcagcatg aagaaaaacc
    15781 cgctcaacac gtccttgcgc tccgccaccc aggccaccga ctccacgcgc agcggatgca
    15841 acgcaaacag ccccgccacc cccgcgcaca gccagtcgcg cgcggccggg ctcattcctc
    15901 ccgccgcgct ccgcgtcgcc cgccgcagca ccaggaacaa caataccgcg ttcgcggcat
    15961 ggagcagcag gctcgtcagg tgatgccccc acgcctgcag cttaaaaagc tgcacatcca
    16021 actgatgcga gagccacgtc agcggatgcc agttgcccgc atggcccgcc gtgaacgccc
    16081 acttcacccc cgcccaactc aagccgctca gcacctccgg gttcgtcgtg acgtagtcgc
    16141 catcgtcgta attgacaaac ccattccgca gtatcggccc aaacgccacc agcgtcgccg
    16201 ccaccagggc tatccccata agcacggcgc ggcgccggtt gccccgcggt tcctccgcag
    16261 tcccgagctg tggcggttgg ttcgattcca tttgcggcca catacaacca taaatacgcg
    16321 caaatgaaca ccgttttccg ggtcggcagg ttccgccgcc ttgaatctat tgacttgtac
    16381 gccgataaca aatggcccct atggcgcagg caacccgggc gggggcgtgg aagtttttct
    16441 cagccaccgg gccaatggcc accatgcgcc gcgtgagggc acgcggcccg tcgggggaaa
    16501 gtgaagcggt tttgaagttt caaaaatggg caagtggcgc ggggagagga ggttgggccg
    16561 ggggcgcggg gcggggcgtg aagttcttcg accgggcagg ggctggccgc atggcgcggc
    16621 gggccgccga agaacacgat atgacgaagc aaacactgat ggaagaggcc agtcatccgg
    16681 catcgcccgc gtatgcgttg cgccgggagc gggatttctg ggaagtcact tacgaggggc
    16741 ggcgcgcccg ttttccgcat gagttggggg cgcaatatgt tgcgtgcctg ttgctggagc
    16801 cgccgcgcga gccgattcat gccgtggtcc tggcgctgaa ggcgcgggcg cggtttggcc
    16861 cgggaggcgg cggggttttg cgggggacgc cgccggtgga tttgaacgag gcggcgacgg
    16921 tgcaggcgtt gtggcggcgg cagcgggagt tggagcgggt gctggcggac cggtggacga
    16981 ttgagccggt caaggccgag gcgctgcggg agctggagga ggtgacggag tttctgcgcc
    17041 ggagctcgtg gccctgccgg cacggggcgg cgcggcgcgc gcgggcggtg gcggtggcca
    17101 ttaagcgcct gcatgcccac ctggccgggg cgtccgaggc cgaccggcgg tcgcacgcgg
    17161 tgctgacggc gttttcacgg catttgtatc atcacctgct ggttccgtcg gggcgcggcg
    17221 ggcggcatgt gcgcggctgg ccgacgacgc cttgtccgcc ggggtgtttc acctacgcgc
    17281 cgccggcggg cattgtgtgg caggggggcg gcgccgggcc ggacgcggaa gggcggggct
    17341 tgaggttgaa ggtttcgcgc tggccgctgc gggggcggtg gtggccgggg cgcacggcgg
    17401 cggcggatta cctggcgcgg tttctgagcc tggcgtccgc gacggcgctg gtggcgtgcg
    17461 gctgcgccgc gccgcgtccg ttcaaggggg gccgggcggt ggtaacgcgc aagccggcgg
    17521 gggtggtgga gcagacgctg cagcaggggg agaacgcggc ccaggccacc cggcagaacg
    17581 aggagagcgt gaaggtgcgc agttatacgg tgccggccgg ctcgcggctg gagcctccgc
    17641 cggcgccgcc cgggacggcg gcggggccgg agggcggggc ggcgcccgcg acggcgtttg
    17701 tgctgagcgc gccgatggcg gtggtcgagc gggaggagag ccgcgcgcgg acggagctgg
    17761 gggcggcgca gaaggacacg gcgcgggagc tgggggcgcg gctggcgagc ctcaagggca
    17821 ttgtctgggg cggggtgggg ttgttcgggt ttgggctggc gtcgctggtg tggccgccgc
    17881 tccgggtggc gatcgggagc gtgacgacca gcgcggcgat gctgctgggg ggcctggcgc
    17941 tgatggttct gccggcgatg gtggtgggca atgagctgct ggtcctgggc ggggtggcgc
    18001 tggcggtggg cggctggttt ctggcgcatc ggcacgggcg gttgcgcggg ctggtgacgg
    18061 cgaacggggc ggcgaacggg acggcggagc cgccgcccgc cggcggcgcg ccgcgctgag
    18121 ggcgccgatt tagtgatgga taatttgggc gcgcgcagtt gggagggttc acggcggcgg
    18181 accgccgggg aaccccgcgc gccaggtcaa aacacataac atagaaaggg ttagcaagat
    18241 gaagatatta gacataccgc agtccggaaa acgcgggctg tatgtttcgc agaacagccg
    18301 gaacgggttg atcagccggg cgctggtcat tccggccaat ccccagacgg atgcgcagtt
    18361 gcgcgtgcgt catttcctgc agacggtggt gacggcgtgg ggcagcacgc tcacccaggc
    18421 gcagcgggat gcctggacgg cggaggccgc tcagcgccgt tcccgggcgc ggctggggca
    18481 atcggggccg ctgaccgggg tgcagttgtt cacgaagatc aactgcgcgc tgctgatcat
    18541 tggcagcgag gtcgtcctga ctccgcccgc cgcgccggtg ttcaaccggc tgccggtgac
    18601 ggggttgagc atcaccaaca cggggggcgt ggtggctttg aagctgacga cctccggcac
    18661 gccggcggat gggacgatgc tgtggggcgc caagccgtgc agccagggaa tcagcgccgc
    18721 gccgggggtg gtgtttctgg ggacgctgga ttcgccggtc aacaacgcga ttgacatcac
    18781 ggccgcctac acggccaagt acggctcacc tgccgcgggc aagaaggtgt tcgtgaaggt
    18841 caatcagaac atcaatggct gggaggatgt gccccgccag ttctgggcga ttgtgccggc
    18901 ggccagctaa accggacggc tcccgggcgg cggccggttg agccggacgc ggttcgggtc
    18961 gagggcagtc ccgcagccgc ggggctgccc tttggccggg agcaactttg aacttatgtg
    19021 cctgactctc tttcaccctc aaacgagcgg cgctccggtt gcgggcgcgc ggccgggagg
    19081 gagttgttga tgtccccctg gcatgacatg gtcacctcgc ccccggcgga ggggcagttg
    19141 gtgctggcgc ggcggctgcc cggcgattgc ccgcccctgg ccgcccgctg ggacgcggcg
    19201 cgcgcgcggt ttcaatgcgg cccggagcgg tggggactcc cctggcaatt cgtggcgcac
    19261 tggcgtccgc tggccgccga tccggcctgg ccgcggcccc gcgccggaac gtctccctgg
    19321 caggatgttt acctgtgccc gccggcgccc gggcaatcgg tctggctgcg gcgggggggt
    19381 gaggatacgg cggcgtttcg ggcggtgttc gaccgggcgg gcgcggtgtt cacgctgccc
    19441 aacggctggc ggatttcctg gtatgaggtc tcacgctggc gcggcggtta accggccggg
    19501 ggcgcggggc aggacggccg cgcggggcgg ggaatgcctt tgacttcgcg cggacgcgac
    19561 ctacactggt agtttaacgc aacagtaaga tgctgacaga agacaacatt cggaatgcct
    19621 tgcgggaggt taaatatccc ggctacagcc gcgacattgt atcctttggc ctggtcaacc
    19681 aggtcgccgt gaaagacggg gcgatcaatg tcctgatgca actcaccgtc gggcggccgg
    19741 acattgccgc gcagatcaag gcggacagtg agcgggtctt gaaggcgctg cccggggcgg
    19801 cgcgggttca ggtggaggtg cgttatccgg gggcgggcgc ggcgggggga ggcccggcgg
    19861 cgacggggcc caacccgtgg gagcagcagc ggcgggttcc cggcatccgg cggattatcg
    19921 ccgtcgccag cggcaagggc ggggtgggca agtccaccgt ttcggtgaac ctggcgtgcg
    19981 cgctgcggca gttgggggcg aaggtggggc tgctggattg cgatatttat ggtccgacta
    20041 ttccgctgat gatgggggtg cgccagatgc caaccctcag cgcccaggac aagatggtgc
    20101 cgccggaaaa ccacggggtg aagctgatga gcatggggtt cctggtggag ggcgaccagc
    20161 cggtgatctg gcgcgggccg atgatcaaca agaccattca gcagttcttc agcgaagtgg
    20221 aatggggcga cctggatgtg ctggtggtgg atttgccgcc gggcaccgga gatgcgcagc
    20281 tttcggtctg ccagaccgtg ccgctggacg ggggcgtgat tgtgaccact ccgcaggagg
    20341 cgtcgctggg cgtggtgcgc aaagggatcg ggatgttcca aaaggtcaat gtgcccatcc
    20401 ttggcattgt ggagaacatg agctacttca ccacccccgg cggggagcgg gtggaaatct
    20461 tcggccacgg cggcgggcag gccgaggcga agcggcagca ggtcccgttt ctgggggaag
    20521 tcccgctgtt caccgagatt cgggaggggg gcgaccgggg ggtgccggcg gtggtttccg
    20581 gcgcggatca accggccggg aaggtgtttg tggagattgc ccgggccttg cgccagcaac
    20641 tggcctgagc gggcaaaggg ccgtcggggg gagagggcgg aaccggccgc gccggcacgc
    20701 ggcggcggcg gaaaaatccg gccgcgggaa agctgaaata aggcttgtca ttcccgggcg
    20761 gcgtgctagg tttctttact cgtaagattt ggactcgcga ttatgtctga cgaaatgaaa
    20821 gagcggcctt cgggggacga cctgctgaag aactcctcgc tctaccggga attccaggcg
    20881 gagcgcgaag agatattgaa gcacaagtgg attgaatccg agaaggcggg tcacgacata
    20941 ggatttgagc gggcgctgac ggattggatc atcaagcacc gcgccaaatg gcgcaagagc
    21001 cagcagacgg cgaatcgcta ggcgccgttg ctcctttaac gccgccccgc gagacggcca
    21061 aggaaccatg cctcacctga acttccccag cgccaccgcc caccggtcat ccggcgcgcg
    21121 gcggcggccc gttccgcccc ggcctgccgt tcccgcccat gcctgacccc accctgatcc
    21181 tgaatgcgcc gacgctgcag cgggcgctga cccgcattgc gcatgagatc gcggaacgca
    21241 acgaagccgg gagcgaggtg gtgctggtcg ggatccagcg cgggggcgtg ccgctggccg
    21301 agcggttggg ggcgctcctg acggagattt gggggcgggc ggtggcgacc gggcgcctgg
    21361 acgtcagcat gcaccgggac gacctggacc ggcgggtggc gccccaggtc cagcccacgg
    21421 tgattccgtt tgatgtgacc ggcaagaccg tggtgctggt ggacgatgtg cttttcagcg
    21481 ggcgcacggt gcgggcggcg atggacgcgc tgaacgattt tggccggccg cggcgcattc
    21541 aactggcggt gctgattgac cgcgggcacc gcgagctgcc catcaaggcg gattttgtcg
    21601 gcaagaacgt gcccacggcc cgggcggaga gcgtccgcgt gcggctggcg gaaacgggcg
    21661 gcgcggacga ggtcgtgctg gagaagcccg tatgagctgg aatcgcaagc atctgctgga
    21721 cattgaatcg ctgacggccg cggagatcac caccgtgctg gacaccgcgc tggccttcaa
    21781 ggcggtgggc gagcgcgcga tcaaaaaggt gccggcgctg cgcggcaaga ccgtcgtcaa
    21841 cctgttcatc gagccgtcca cccgcacccg cacgagcttc gagctgtccg agcagcgact
    21901 ttcggcggac atcatcaact tcaccgccga ggcttcctcg ctgcggaagg gcgagaccct
    21961 caaggacacc gcgcgcaacc tggaggcgtt gaacattgac ttcatcgtga tccgccacag
    22021 cgcctccggc gcgccgcatt tccttgcgcg cgtcctcaac gccagcgtca tcaatgcggg
    22081 cgacggcgcg catgagcacc ccacccaggc gttgctggac accttcacca ttcgcgagaa
    22141 gaaggggcgc cttgcggggc tgaaagtgac catcctgggc gatatcctct acagccgggt
    22201 tgcgcgctcg gatatttggg cgctgaccaa gctgggcgcc caggtgacgg tgtgcgggcc
    22261 gtccacgctg gtgccgcgcg tgttcgagca gatgggctgc cgcgtcaccc ataacgtcga
    22321 ggaggccatt cgcgaggcgg atgtaatcaa cctgctgcgc atccagcacg agcggcagcg
    22381 caagtccatg ttccccggcc ttaatgaata cagcagcctg ttcgggttga acaaggagcg
    22441 gctggcgttg acgaagccgg acgtgatgat catgcacccg ggcccgatca accgcggcgt
    22501 ggagattgac agcgaaatcg ccgactcggg ccgctcggta attctggagc aagtgaccaa
    22561 cggcctggcg gtgcgcatgg ccgtgctctt cctcgtcaac ggcgggaagg ggccgcagga
    22621 agtggctcct tgaggacgcc tgagccggaa tagtttccgc ctgcagggcg ggtgttcgcg
    22681 ggagcgccgc gtccgcaggc gaggcccccg tggcggaagc ccgcccgcgg cccgcgcgcg
    22741 gaagcggctc ctttcaccca aagaccagcc ggcacacaat caccgaggcg atgacgccga
    22801 cggtgtcggc cacaatcccc gcgggcaggg cgtagcgggt gcgcttgacc ccgacggagc
    22861 caaaatacac ggccagcaca tagaacgtcg tttccgtgct gccgaagatg gtgccgccgg
    22921 tgcggctgat gaggctgtcc ggcccgtatt gcttgaccag ttcggccagg agccccagcg
    22981 tgccgctgcc gctcaacggg cgcatcagca ccattggcag cagcggcgac ggaaacccca
    23041 ccgcctccat gaccggggcg agcgcccggc tgatgaggtc aatgcccccg gcggcgcgga
    23101 acatgcccat cgccacgaga atcgccacca ggtagggaat gatgcgcagg gccacgtcga
    23161 agccctcttt ggcgccgtcc acgaactcct cgtaaacctt gacgcggcgc aacgccgcat
    23221 agagcggaaa gacggaaagc atgaagggga tggaaatccg ggagacggcg tccacgatgc
    23281 ggatgaacac ggtttggccg gcggcatccg acggcggcgt tagccccacc acggccggga
    23341 acgccaggcg caggaacacg tagagaaaga acagcccaaa cgcgctcata atcagcattc
    23401 cccaccaccg caaaggagcg aggtcggccg cttcccgaat gggcgtcttt tcgttacccg
    23461 cactggctgg cgccggcgcg gccggctcgg gggcggattc cgcgggcggg gcgggggcgg
    23521 gcggcaggcg ataaaagggc aggcgctgca ggattttagc cgcggtcacg gcggccaccg
    23581 ccgcgcaagt ggtggcgatg atggaagtgc ccacgatggc ggttgggttg ctggatttat
    23641 tggcggccag gatggcaatg gcggtggcag ggatgagctg gatggaactg gtgttgatgg
    23701 ccagatacgt gcacatggcg ttggtggccg tgcccgggcg cggattgagc gtttccaggt
    23761 gccgcatggc cttcaagccg agcggggtgg cggcgttgcc gaggccgagg atgctggccg
    23821 ccatgttgag cagcatcgca cccatcgccg ggtggtcgtg gggcacttcc gggaaaagcc
    23881 aaaccatcag gggccgcagc aggcgcgcca gcgcggccac cagcccggcc cgttcggcca
    23941 gccgcatgat gccgagccag agcgccatga tgcccaccag cggcagcgcc gttcgcatca
    24001 cggcaaactc cgccatctcg aagctcttat cggcaatcgc tttcaggttg tccgtgcagc
    24061 cgccgatgat cacggccagc acgaccagcc caagccagat gtaattgagc atcccgcgca
    24121 aattacccgc acgggcgtgc gccgcgcgag taaaaatcac cgggggcgtt tgccgcggcg
    24181 ccgccggggc tggcgggcgg gacacccgaa aacccgcgat caaggactga tgcggatgac
    24241 ctcttcgctc tcgtcaaaca tggtattccg gtcgaagttc agccagtgct tgagcgagac
    24301 gatgcgcact tcctggaagt aggatggcgt caagccgcca tgcttctttt ccaacgcggc
    24361 catgtcttcc gcgatgcgcc gggcgaattc aggcttcaag ctcaaccgca accggcgttc
    24421 gccatagcgg aagatggctt cgcaggcggg cggcacgttg gtgggcacgc tgatggtgcc
    24481 gcggccaagg ctggcgccgg tggcggtttg cagcccgtca ttgaggcagc tcagcggcgg
    24541 tttgaggccc gcgtggctct cgacgcgcag ttcgtccagg gccacattga accgctcccg
    24601 cgcccgcaaa cccatcttgg cgccgacgat cgagtacgtg cccaggtgac gatgcaattc
    24661 gctggtcagc accgcggctt tccattcttc caggccatgg cggctgatta tttgcgcggc
    24721 ggattcgcgc acatccggca gcaggtcttg tggtttaacg gggaactcgg ccataatcac
    24781 cgtggcgcgc tcggggaagg cccgcaacag ggcgcaaaaa gaccggcgca ccgccgcggg
    24841 atccgccggg gtccattgcg accagttggg ctgccccgcc acgggagtca gcgtgcccag
    24901 ggccgggtcc agaaaacgca gcgcgaccag gtcgtcccag agccgcaggt gccgggcgcg
    24961 gaccagctcg gcgccgcggc cggtgctatg cagccacacc accagctcgg ccgccggcga
    25021 atccaggccg gcgacttcat ccagcagggc gtcatccagg accggcgcca cggtttcgtc
    25081 gggccaatac accacttcga cacgcaggcc ggcggcgatg accttctcac gggccgcctc
    25141 gtcccgcagg gcattccagc cggccgggct gactttcggc ggcgttccat accacagcac
    25201 ggagcggatg cgcgcggcca ggccgggatc gccctcgagg gcgtccgcca ggttcgacag
    25261 cggcccgagg cagaggtaaa caatggggtc cggagcggct ttggccatca atcgaatcag
    25321 gcttgcggcc tcatgcaagc cggtgcgcgg aatccggggc tcgcccagtt tgtcccagtc
    25381 caacgctgtg gcgttggtgc gaaacgaggg cggcggcgct gtttgcttgc gcccgaggcc
    25441 gacggccaca tcgctttgtt tgagaaaccc cagcaggcga caggcgttgg tggcccccac
    25501 atcgggcggg caaacgccgt cgctggtgac aatcgccagc acgttgaggt aagggcaatt
    25561 caacagcagc gccagggcgc ggacatcgtc cgacgccata tcggtgtcca cgatgacgga
    25621 cgggccatcg tgggcgctgg ccaggcgggt caacaagccg aatagcagca gcccccggga
    25681 gagccgtcgc cacatccgcc cgcggatgct tccgccgggc agccttccgt tattgagtat
    25741 ggaggtcata aatggtttcc tgcctaccaa tgaagccggc gcgcgcggag gtattccagc
    25801 aagcgctgcg cttcgtcgga ttggatgatg tttgcgccac gggcgacaac ccaaccccag
    25861 ccggcctccg ggtccgtcag ggatttggcg tcggtatgtc ccgccgccag actgtcccaa
    25921 agagtgttga tccacacccg ggcgcccgcc tgcttgatcc gggcgatggt ttccggcgcc
    25981 acaatcggga cgtcatcgtc tgcaaaaacc agttcaaaag cgcaggggcg aagccggcgc
    26041 gtgtagggtt caattttcgc ccagcctgat tccaggttat tctccgggcc tttcttttga
    26101 aagatgatcg gcataaagag cacgggcggg tcgaggtcgg caaagtccac ttcgcaccgg
    26161 gcggcgttat aactggattt gaagatggcc tggcgggtcg tgccggtctt ctggaggatg
    26221 gcgcggcatt cggggacgag gatccaggcc ttatcgagat tgaccaggca cttgccccgg
    26281 accaggcgca tgacttcctc aagcgttggc atctggtggt tggtgaccgg ggcattggtg
    26341 ccgccgacgc cgagccggag gcgcagccgc ttgatttcgg cgagggtcag gtcgctgatc
    26401 gcgccggtgc cgtcggtggt gcggtccacg gtggcgtcgt ggcacaagac gagctggccg
    26461 tcccgggttt tgcgcacgtc cacttccaca atatcaatcc cctttgcaat gctgtccgca
    26521 atcgcggcca gggagttctc ggggtatttc tcccatgcgc cggtcgaacg cccggagagc
    26581 ccgcggtgcg ccgcgacgag cacctgggtg cccgccggtt gccggagttc ggcgcagatg
    26641 cggctggcct tggagggcgc gactttcgtc gaaacgcagg cggccaaacc cagcgccagg
    26701 ggcaccagga ccagccctcg gattcggcgc cggaggggag cgtgatttcg gggtgagatt
    26761 ttcgaagggg tagcagtcat tttattcatg ggttttgacg cgccacagcg acgccgccag
    26821 caccagcgac agcaccgatg cgccaatcca aaaggcgatt gcggagcgga agtcgtagtg
    26881 cttgacctgc gtggcaacgg cttgtccttg ttcgttaagg gcggtgacgg ttgcgaaggt
    26941 gaccccacgc tgaatgagga gcccgctgac ccactcctgc agcgcgttgc ctaaatagct
    27001 gacaatgccg ataaagccca tcgccgcccc cgccgcccgc cgaccggcga tatccacggc
    27061 aaataatcct cccaggacag ccagcaggcc gctgagcgtg aaaccgaaga ggataaacgc
    27121 cgccgacaac accaccgggt ggccgggcgg cgtgtagaaa atcatcaaca gggcgatgac
    27181 ttccagcacg ccgaagataa aggtgaccgg cggccggcgc gcgttgaaga accggtcgga
    27241 gatgaagcca tagaccacac agccggccag tcccgcgccg gtgttcaaac cgagcatcag
    27301 ccccgcctca accagcgtgt agttgcggtg ctcctggaga taaagcatgc cccaactgtt
    27361 gatggcatag cggctcacat acatgcaggc gctggccagg ccaagcaccc acatcgccgg
    27421 catccgcaac gcccgaagct gatcccgccg gggatccgca gccggcaccg cccggtctgt
    27481 ccccgccggg tgatcgttgc gccagtccgc caccggcggc aggcccatgg tttccggccg
    27541 gtcgcgcagc aacagcagga tgccaaacgc aaccagcagg cagaacatcc ccggcccggc
    27601 aaagcccgcg cgccagccca ggtgggtcac cagcagcgaa ttgaacacga acgtcagacc
    27661 ctcgcccagt gagtgggaac tgctgaaaat cccataatag ctgccgcgct ctttggcgct
    27721 gaaccagttg gccagcgata ccccgctgca cggcgacccg gcgccctgaa accatccgtt
    27781 caacccccac aaaatcgccc aagcccacgg cgagacaaag ccgagcatgg ccaggttgat
    27841 caaggccgac aacaacaccg tggcggagaa aaaccggcgc acgttggcgt ggtcggccag
    27901 aaagccgttg accagcttgc ccaccgcgta gccgatcgag aacgccgagc cgatcagccc
    27961 cagccattcg gcggtgaaca ctccttcgtc cagcagcggc tttttcatca ccgccagcgg
    28021 caggcggcag gtatagccga acccgtagcc gcaggtgatt gcgaacatca ccgacatccg
    28081 gtggcggcgg tatagctggt tgacgcgctc cggatcagtg atccgcggcg cgtcggggcc
    28141 ggtcgcaaac caccgcagcg gattccaact ccgcgtggcc gcttcacccc ccgcgccact
    28201 catgccgggc caactcccca tacgcctgtt ggccttcggc cagcctgcca ttcgcgccgc
    28261 cacatgcgct aggtttgctc cgagtcggtc gcgtagccgt ggaccttggc gggccaaacg
    28321 aacgcgaaca agagcatgct caagagcgcc acccccgtca ggctgcccag cgttatgttc
    28381 cagccataat gctgcaccag cgtgcccagg ccccaccccg aaaacaacgt gctggcgtag
    28441 gaaaaaatgc ccgtcaaccc aatggccgtc gccgccagcc gcttggtggc caggttggcc
    28501 accaccacgc ccaccagcgc ctgcgggcca taaaggaaaa agcccgccag gcaaagcggc
    28561 aacgtgcaga tcaccggcga gcggctgggc agcttccaaa acagcagcac ggacaacgcc
    28621 gtcagcagca tcgaaaaaac acacaaccgc gcgccgcgcc cgccaaagag ccgatccgtc
    28681 agccagccac tgaccagcat cccggcgacg cccgacactt cgaaggcggc caccatccag
    28741 cccgcgtgtt tcaattcgat gtgcttcatt tccgtcagaa aggtggggcc ccagtcgagc
    28801 accgcatagc gcacgatata gacgaagaaa ctggccatcg ccaacagcca gatgtatttg
    28861 ttgccgaaaa cctgccggag ttgcgcggcg gaaaagcccg gcgaatccga aggcccgctg
    28921 gcggtgcctt ccacttcggg caaccccagc gagggcggcg tatcgcggag cgcccccagc
    28981 aaaaacagcg cgcacagcaa cgccagcccc gccggcacaa aaaagcacag ccgccaccca
    29041 tagccgacca ggtagccgca cagcaccacc accagcccgg ccccgatgga atgggaggtg
    29101 ttccaaatcg agaaattggt cgccagctcc ttcggcggaa accagtgcgt catcagccgg
    29161 gcgcatggcg gaaagcccat gccctgcacc cagccattga gcatccacag caagcctagc
    29221 gtgacgacgg cggaactgaa gccaaacagg atattcaccc ccgccgaaag gatcagcccc
    29281 agcgccataa acgtgcgcgc gttggtgcgg tcgcccacaa atccgtgggc aaacttggaa
    29341 aaaccgtaga gcagcccgtg cagcgtaagg aacagcccca ggtcggattt ctcgatgccc
    29401 aggtcccggc tcatcgccgg catcgccacg ctgaggttct tgcgaacgaa gtagaacatc
    29461 gcgtagccaa caatcgtgga gaacagaatg cggcgctgcc aataacgata ctgcgccttg
    29521 atctccgccg gatccgtcag catcgtttcc gccggcgccg ccggggcaaa aacgcgcaaa
    29581 atcgccggcc attgcggctg gcgcttcgtg gttgcggtat gcgagtccgg catggaatca
    29641 ctacgcggac gccattcccg ccagaacaaa cccaataaga tatctcaccg gcgcagcgtg
    29701 ccccggatcg tccaaggcgt caagaacaag gcggccaacc ccgaacagcc cgggcgcggc
    29761 gcggttagaa cattgaactg gacggctatt tgatggtaca ctgaaccgca ttgaagaagc
    29821 gacgacttgc gccagtcccg gtaagtttgc cccaggatga gggagacgaa ctgccgctgg
    29881 aatttgcgcc gccgccgccg tcggcaaccg cgtcggcggc ggcggtggca tgctatttta
    29941 acgggcgtcc cctgccgccg gatgccacgc ctatgcggct gttgggggtg gattatgtgc
    30001 atattccgac ggctaatggc ggagacctct acctgacgcg attcggactc ccgtttcagg
    30061 agcacttgca actggagaac tggcgggacg aagcgtggtt cgagacccaa tgcgagcggt
    30121 tgcggggcac cagcgtggtt tatcgcgttc ccaccaagcc cgtccacggc cggtccctct
    30181 cgctggtggt caagtattgc cgcgtcggcg aggatgtgcc gttggacacc atgacgttcg
    30241 agaagttctc ccaggccgaa ttcaacagcc cgtatgagga gttctcgctg gtaatggaac
    30301 tgcgcgagcg aaaggtagcc gagcgcattc tcacgcaccg ccccctggcc atctatatcc
    30361 cgcccgaacg gctggaactc tggcaaacgg ggcgcagcgc gcaccgaatc gagcataaga
    30421 aggcgaagtt ccgggatgtg gagctggaca tcttccgccg ctacgtcctt atctaccaat
    30481 ggatcaacgg tgaatccgcg gacgccgcgc tcgaaaaggc ggtgccgggc gccgcgcggc
    30541 gggcggagga gttgaagcgc ctgacgctgc gggccaaagc ggaaatggca cgcaacggtt
    30601 tcatggtggc ggatcacaaa ccggcccacc tcatcctccg ctggcgccgc gacggcaccc
    30661 tgctgcggaa gaagaacggc gactacgcct atgcgcttat tgacttcgag ttgctcctgc
    30721 gcacccccga acacgagcgg gaaacccagt cgtcgcgccg cgcgatctat ttaagccggc
    30781 agcgggaccg ctttgccagc cccaagggct tgcaataccc cgcccacctc aaacccgtgc
    30841 gcctcctggg ggtggactac gtatggggcc acgccgacag cacgcacggc agcctgtggg
    30901 tcgtggggca ggacccggac ctgttcgatt attttgtgcc ggaacggtgg cggcatacgc
    30961 ccagcaaacg gctcagcacc acaaatgaaa cctattatac cctcaccaag gatcgcataa
    31021 acctggtctg gaaagtctcg cgcgtgggcg aggcgccaga ccccgccgcc ggcagcccgg
    31081 gccccggcgc gcgggcgcac ccgggctaca acagcccctt cgaagaggtg gccatcgcgc
    31141 tggacctgaa agcgaaaggc attgacgcca tttatccgcg ggcgatctat atgtccggcc
    31201 tcaagtccac tcgctcgaag ttatacgtca gcgacaaccg ccgctaccat tcccaccagg
    31261 cgctgaccac acccgatggc cagccggtgc tccggccgga ccacatttac ctgaccattt
    31321 ggggcttctg gaacgggctc gatgaaatcc tggccagccg ggatgacgcc tattgcctgg
    31381 gcatcagcct gctggaggcc tgccaggacg gcattatcag cgcccgcgac gcaaaacgat
    31441 tgcttgcccg ggaacagaaa aagctggcgg ccgcgggcta ccgggatttg tcgcccaaac
    31501 ccacgcatta tctgctgtcg cttcgccatt cgggcaccct ggttcgggat gatgacgggt
    31561 ggccaacgct gcgcatttgc aatttcgcgc tgatccagag tgccggctcc gccaaaccgc
    31621 ccggcccggc cccgggcgcc cccgcgggaa cccaccgcaa aaagggctga cccgggcggg
    31681 catccctgct accctacccc ggtcgctatt tttgcagagc gcatgacagc aattttgggc
    31741 atctcggctt tctaccacga cagcgcggcg gcgctggtgg tggacgggga tattgtggcg
    31801 gcggcccagg aggaacggtt cacgcgcaag aagcatgacc cggggttccc ggccaacgcc
    31861 attgcctatt gcctggcgga aggcggcctg ccgccggagg cgctggatta cgtggtcttt
    31921 tacgacaaac ccctgaccaa gtttgaccgg ctgctggaaa cctacctggc ttacgcgccc
    31981 gcgggattca agagctttcg gctggcgatg ccgctctggc tcaaggacaa gctgcatctg
    32041 cggcgcacgc tgcggcagcg gctccgcgcc gcccggcgcg cccggctggt tttcaccgac
    32101 caccatgaaa gccatgcggc cagcgctttc tttcccagcc cctttgagcg cgcggccatc
    32161 ctgaccctgg acggagtggg ggagtggagc acggccacgt tcggcgtggg cgagggcaac
    32221 caactgcggc tgctgcggca attacagttt ccccattcgc tgggactgct ctattcggcc
    32281 ttcacctatt actgcggatt caaggtcaac agcggcgaat acaaattgat ggggctggcc
    32341 ccctacggcc agccgatcta tcaggaggcg attctccggc acctgattcg gctccagccg
    32401 gacggcagct tttggatgga catgcagtat ttcaactact gcgcggggct gacgatgacc
    32461 agcgaacggt tccactggct tttcggcggg ccgccgcgct ccccggaatc cttgctcgaa
    32521 caacggcaca tggacctggc cgcgagcatc caaaaggtca ccgaggaaat catgctgcgc
    32581 atgacccggg aggtgtggcg gcaaacgcaa atgaaaaacc tcgtcctggc cgggggagtg
    32641 gcgctcaact gtgtggccaa cggccggctg ctgcgcgagg ggcccttcga acagctctgg
    32701 atccagcccg ccgcgggcga tgcgggcggc gcgctgggag ccgccctgtt cgtctggcac
    32761 catctgatgg accggccgcg ccccaccgat ggacaggatc gccagaaggg cagcctgctg
    32821 gggccgcgct acgataacga acagatccgg cagttcctgg caacagcgga agccccgcat
    32881 cagcaataca ccaacgaaag cgaactgctg gcggcggtgg cgcagatgat ggcggagggc
    32941 aaaatcatcg gctggttcca gggccggatg gagttcgggc cgcgggcgct ggggtcgcgc
    33001 agcattattg gcgatgcgcg caacaccacc atgcaggcca ccatgaaccg gaaaatcaag
    33061 ttccgggaaa gcttccgtcc cttcgcgccg tgtgtcctgc gggagcgggc gggcgattac
    33121 ttcggcctgc gggcggacca ggaaagcccc tacatgctgc tggtggcgcc ggtgctcgaa
    33181 aagcatcgca cggcgctgtc agccgccgac cggcaaacga tggccgccgc cccggacctg
    33241 cgccagcgcg tcaacatcgt gcgctcgacg gtgccggcca ttacccacgt ggactacagc
    33301 gcgcgggtgc aaaccgtgga cgaaaagcgg cacgggcgct actaccggct gctgcaggct
    33361 ttcgagcggc tgaccggctg cccggtcatc gtcaatacca gcttcaacgt gcggggcgaa
    33421 ccgatcgtct gcacgccgca ggaagcctac cgatgcttcc tggccaccga catggacgcg
    33481 ctggtgctgg aggattttgt gctgcgcaaa gaacaaatga accaggcggt gaccgccgcc
    33541 gaacggcaaa aatacctggc ccaattcgag ctggactgaa cccgctttca aagccgccca
    33601 tatgccccta taatccaacc gcatgacctg gtccgacatt ccctttcagc cccccgccaa
    33661 ggccctgcgc cagtttggcg ccgcctggct gattttcttc ctggcgctgg gcgcctggca
    33721 atacttcgcg cgccagcggc cggtgctcgg agcgatcctg atgggcctgg ccctggtcat
    33781 cgggctgctg ggactgctcc gcccggcggc cgtgcgctgg ctgttcgtcg ggtggatggt
    33841 gctggccttt cccattggct gggcgatttc ccaactgatg ctggcggtgc tgttctacgg
    33901 cctgctcacg ccggtggcgc tgtttttccg gtggcggggg cgggacctgc tgcagcgcaa
    33961 ggccgccccc tccgcaacca ccttttggct gtccaaaacc acgccgtcgg acgtgcgcaa
    34021 ttatttccgg cagcactaag cccgccacgc caccccagac aatccccatg caaaaacaac
    34081 cgcaaaatga gtttgaaaag gccgccgccc aacccccgcg cggcggatta atcgtcgaac
    34141 tggtggagtt cctgcggcag aacaagaaat ggtggctgct gcccatcctg attctgtcgc
    34201 tgctgctcgg cctgctgatc gtgctgtccg gctccggcct ggcgccgttc atctacaccc
    34261 tgttttaacc gttctacatc gtccggcgcc catgaacctc ccctggcagc tttggcgccc
    34321 cgccgaaacg cggcgcggcg cgcccactgc gccgccgcgg caggagggct ccgcgcttta
    34381 tgaagatcgg gttgcaacgg gcggttgacc gttatgccgg ggtgcccgcc tgtgcggcgc
    34441 tctcgctcct gcaccggctc gggcccaagc cggttgccgc ccggccgccg cgccggattc
    34501 tggtgatcct gctttccgaa atgggcagcc tggtgctgac gcagcccatg tttgcccatc
    34561 tcaaagctcg ttacccggac gcctccattc acgccttgct gttcgcccga aaccgcgccg
    34621 tgctggactt gctggcggtc ctgccggggg aaaggattct gaccctcgac gaccgctctc
    34681 tatggcgcct ggccgccggc agcctcaaaa tcctgcgcac cctgcgcgcg ctccagttcg
    34741 acgtggtcat tgactgcgaa ttattcgcgc gggtcagcag cctcctctcc ggcctgtccg
    34801 gagcgccgct gcgggtggga ttccatcgcc acacccagga agggctttac cggggatcgt
    34861 tcatcaaccg gccggtgctc tataatccct accgccacct ttcccgccag ttcctcacgc
    34921 tggccgccgc catcgaatcc gaaaccatgc cgccgggcaa ggacgcgccc gacgcgccgt
    34981 gcgagccgcc gccgcggctg gcgtttcccc ccgaagaact gcggcaggtg gcggcccaac
    35041 tgcacgccga cttccccgcg ctcccgggcc ggcgcctcgt gctggtttat cccggcggcg
    35101 ggcttttgcc gatccgggcg tggccgcgcg agcactattt tcagctctgc gcggccctcc
    35161 tccgggaagg gcatgcggtc ggcattatcg ggctgccgga cgacaaatcc ctcgggcagg
    35221 caatcgtggc gcactgccgc agcccaaact gcgttgacct gaccggctac acccgctcgc
    35281 tgcgccacct gctggccctc tttcatcacg cggcgctgct catcaccaac gacggcggcc
    35341 ccgggcaatt cgcggcgctg acccccctcc ccgccatcat cctgttcggc ccggaaacgc
    35401 cccggctcta cgggccgctg gcaccaaacg cattctgctt ccatctgccg ctgccctgct
    35461 cgccctgcct caccgcctat aaccaccggg cctccccctg cgatggagac aatcagtgcc
    35521 tgaaacggat caccccggag caagtcctgg cgcaggcgcg cgcgatgctc cgcaccgccc
    35581 cgggctgacg gcgcgcgccc ccgggcgccc ctatttccga tagctggcca ccacccaatc
    35641 gctcagaact ttgtccggcg ccgccaccgc gtcggcgtca atcacgaagt cccgcgggcc
    35701 ggagttctga acgatgagct tcaacccgaa ctggtagtcc ttgaaccgct gggcgcagag
    35761 atcgcgcacc ggctgccctt gctccgccgc ttcccgcacc agccgatccg cggccgcttc
    35821 cgtgaagcgc aggcggatgc cgtgcgccgc gtggaagcgc cgcgcaaact catccaccag
    35881 ttgccgcatc accatccgct cctcccgcgc cggatccgac aagatcttcc gcagctcgcc
    35941 cgccggatcg tccaccagct cgcgcgtgac cacaaaccgc ttcaccgggg tggagggcag
    36001 ttcgaacttg atgttgcgaa acacccgctc gcagaccgtc atcaaacccc gcgccccggt
    36061 ctgctcttcc gccgcccgct cggcaatccg ccgcaagccg ggatcgtcaa aaagcacctc
    36121 gatgccgtag gcggcaaagt cctgctcata ctgccgaata atgctgccct ccgaactctt
    36181 gagaatctgg aacaggtcgt ccacctggag cgcatggcac accacgcgca ccggcagccg
    36241 cccgatgaac tccggctcga agccaaactc gataaaatcc cgcgtctggc tgtgctccag
    36301 caccgcctcg ggcgacggtt ccgcgcgctc ccgggccgca aacccgatcg tcgcctcgcg
    36361 cagccgccgc tgaataatct gctccaggcc gccaaaggcg ccgctgacga tgaacaggat
    36421 gtgcttggtg ttgatcgtgg ccggcgccgg acggccgcgc tgcgtgaaat ccatcatcgc
    36481 ctggatttgc ccggcgatgt cgctgggcga acgcgccggc acctctgtct cctccatcag
    36541 tttgagcagg ttcgtctgca ccccgcgccc gctgacatcc cgcccgctcg catgggaagc
    36601 ggcggcaatc ttgtctatct cgtcaatgta aatgattcca tactgcgccc gcgtgacgtc
    36661 cccgccggcc agccgcaaca aatcgcgcac catgtcctcc acatcgcccc ccacatagcc
    36721 cgtctcggaa aacttggtcg cgtcggcctt cgcaaacggc accccgatca aatccgccgc
    36781 gcaccgaatc agaaacgtct tccccacccc ggtagggcca atcagaatga tattctgctt
    36841 ggcgtagttc ggggattcct tccccgccag cgccagccgg acatggtgat aatggtcgca
    36901 cagcgcgacg ctcagcacct ttttcgcctc gtcctgccgg atgacgaacc gatccagata
    36961 cgccttcacc tcgcgcggtt tgtagccaaa ctcgaacgcc gccgcccggg ttttatcttc
    37021 gccgccttcc ggcgccccgg ccgccgccgc cgccgccccc gggaaggccc gcgcccccgc
    37081 gaaatgctgc tgcataaact ccgccatccg ccgctggaat tcttccggcg tcggcggccc
    37141 gctaaacact ggtttgctat ccgtcatcac atcactccgc ccggcccgtt cccggcgccc
    37201 ggctcacaag tcagcctaat ccgttttcgc cgcaagacca acccgaagcc gcggcgcgga
    37261 tccggccgcc gccaacctcc gttcacaccc ccggcgcttc ccgcccgtgg cggcgtcccc
    37321 acgcgccacc ttgacccgca accggcgcgg gaacgcccgt atcagcttcc ttgacgggag
    37381 aaagatcagc aaccccgccc tcggttgggc gcaaccgccg gcggtttgcc ctggccgatc
    37441 agccgccacc cgggattccc ccggttgccg gcggcaagtt ttgcccatca gccccggcct
    37501 acttttgcgc gtagtacacg acggcggggg gaatgttgag gcacaccagc gaggtgcgga
    37561 ggctgcgaaa gcatgagcgg attcgccggc gatccagcag cgagtattga aactggacat
    37621 acaggccgcc cggacgcagc acgccgcaga tggcatcaat gagcgcctgg gttcgttcgc
    37681 cgtcaagatt gcccaggggt atgcccgaaa gcacgtaatc caccgggccg gttccgcggc
    37741 ggctgatttc cgaaaggaga tgctcggcgg aatccgaagc cacctcgacc tggtcgccca
    37801 aacccgcttc gtcaagattg tcccgggtct cgcgggccag ggtcgggttg atttcgcagg
    37861 ccaggatgcg cgcctgggga cagcgggccg ccaaccgcag ggtcagcgct ccgttgccgg
    37921 ctcccagctc aatgatgcgc ccggaatagc tttccggcac cggggcgatc atttggctga
    37981 taagaaacct ctgcgagggc acaatcgtgc ccgtctgccg gagtttgagc agggcaacgc
    38041 acataaagtg cacataattt gccactgcat ggcttagtcc tgttcgattc agatataatc
    38101 attgttgggc cagtggtttt tcatacccgt caccggctgc caacccggaa aaactagcac
    38161 gccgccccgg cggatgccaa ttactaattt ggctggcctg gcgaccggcc ctttggttgt
    38221 cggccctcag gtttcggtgg ctgaggagga aaaggttttc tcaaacccaa acccgctttc
    38281 cgggctcagg agaccgggat gaaaacgtct attgcttcaa gacggccgcc cgaccctgcg
    38341 gacagcgcaa gcaatcacct ttcggcaacc tccgcctggg aggggaaatg tcctcgccag
    38401 ccgtgatccc atcccaccca aaaaccaggg cacgcgacaa cacccggccg cccgttccgg
    38461 ctttgccgaa cgttcgcgtc ctctccctcc cgccttgaaa cgtcctttta catcatccgc
    38521 gactcgtcca cccctccatt ccggctcagg cccgcgccca aacccgcccc acccgcccca
    38581 gccagcctaa cgctcccatt aggccgtgta caacgggggt acaaaggagg ctcaaagggg
    38641 gctcatgctt attttccatg ttctcctgtc gggcttccga tgtactcccc ttgtacatgg
    38701 cttgaaagga cggagaagga acggccttgc gggcggcggt ggatgcaggg gcggcggagg
    38761 gcttcgattt cttaaccttt gccgtttcag ccgcttgctg agatttcaac atcggcggcg
    38821 gcgggacgat ggcgttgtga acccggcttt ggtctttcca gccgttccgg gtctgaacca
    38881 cggcgataaa taccttctgc cccggagcgg ggcgtccaaa ccgggccgta tagaggtccg
    38941 tgatgtcgct ttgcccgttc tggcttgggc ccaacaggcc caggtaatag gcacggcggg
    39001 gcttcatccg gcccgcgctg cacggtggct gtccaaagac cataatgtct tcatgggcct
    39061 ccccgacatt cagccgcagc cgaactccgt cttccgcgtc attgacgatc tccag
//
LOCUS       DAOPQG010000022        39107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1383_length_39107_cov_14.836321, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000022 DAOPQG010000000
VERSION     DAOPQG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 39107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39107
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1383_length_39107_cov_14.836321"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..2563
                     /locus_tag="PKI07_03965"
     CDS             359..2563
                     /locus_tag="PKI07_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOC50239.1"
                     /translation="MIERDFDVPFVADLALREKQIQQNYRPVVAVHKWFARRPGTLFR
                     ALLLSEFLEGPLREQFYRGQSLKHLRVLDPFMGGGTTLLEANRVGCSVTGFDINPMAW
                     WIVRQEIGELNPRAYRVAADTLRDTLESEIGHLYRTQCRECGARDAHVKYFLWVKSPP
                     CAKCRQPVDLFPNYLVAADVRHPTNLFVCRDCGELYDSPARKNPPGCPHCKTRFSVEF
                     TARRNRCKCKHCGHVNAYPTPEGGPPPHRMFAIEYHCERCRPAHSGRFFKKPAPADLR
                     RYDEAAARLAAGEARFIPQEEIPPGDETDRLHRWGYRFYREMFNARQLLGLELLCHAI
                     KRQKDSRIADALATNLSDLVRYQNLLCRYDTKALKSLDVFSVHGFPVGLIQCESNVLG
                     ISGKAMAVGSGGWLNIVDKFAKAKAYCRHPFEIRHEGRRKVRVPILTEWIGDQRNDLH
                     PPETKEVALACADASEADFTGRKFDAILTDPPYFQNVQYAELMDFCYVWLRKLVKAAH
                     PEFDGLTTRHTNELTGNVSMGRTLEHFTEGISRVFRRMAKALNPGAPLAFTYHHNSLD
                     AYLPVAVAMLDSGLACSASLPCPAEMGASIHINGTVSSIVDTVFVCRSTGKFPRRWLA
                     DTPAALARVAAGDLRLLEQGQMKPTQGDMRCVVFGHLIRLAVWRLRPTWVATPKAADR
                     MAAVRRWIIKFGGADAVLQELGDAYARARAEQQWELNEEDKAFRTSTDEISF"
     gene            2547..3581
                     /locus_tag="PKI07_03970"
     CDS             2547..3581
                     /locus_tag="PKI07_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50240.1"
                     /translation="MKYPFEADYQEILADSDRFVTAIVTSLASEFLTLPKGKGFIEYP
                     QFAAGYEDLKKVTTGFSKLPRREIVDLVASTPVCFIVIRSILGFTPSEWAYVASQRRG
                     AAVTQGFARTLDRNIRMQPLKPLKCRGLTRERLTALLDAACDLLQEGCPKPNDITIHR
                     LQKADTKEGLQSIKSLARMGAPYAMLLYERFLGRPFAGHRDSVSELVGDSLESAIEDV
                     LSKAGVSYRKTKRAERVPGFDQAPDFIVPDEFTPRVVIEAKITEDDGTARDKVTRIQH
                     LGELSTAGAPVGQPKYQVIACIAGRGFGVRREDMRKMLFATRGKVFTLKTLNRLVECS
                     DLAKFATKPY"
     gene            3878..5197
                     /locus_tag="PKI07_03975"
     CDS             3878..5197
                     /locus_tag="PKI07_03975"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOC50241.1"
                     /translation="MRLGIAVRLGGIGGLLAVWCASATITLEPWAPMFRGVDFARGEA
                     DANEARLQKVFALRVDLHDPTVEFFSTPANGDRPKETDGQTTAAFVQSYGVSVGVNAN
                     FFSPVNYTPNDPRDLNGLAVSRGEVVSAFENGFPAVMITRSNAVGFVSQAPGSLANVW
                     TAVAGSDLILVNGQAKMADCNTEFCKPHPRTAVGLSAQRRYFYLMVIDGRRPGWSDGA
                     TLYETGQWLLRLGAWNGLNLDGGGSSAMARLTNGAAVVMNRPSDWLPRVNGNHLGVFA
                     QPLAPALLAQPTNRTVSVGQPATFHVGVVGAPPLYFQWRFNGVSLTGATEAACTIGSA
                     QMAHGGWYSVVVSNAWGAVVSSNALLSVQEEGSLVGRLFGAQKDGEVFSVWLQADTVG
                     KYSLEWRDLPAESNWSAWPAVNGNGAALQLVDPQATNALRLYRVRRW"
     gene            5454..6398
                     /locus_tag="PKI07_03980"
     CDS             5454..6398
                     /locus_tag="PKI07_03980"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOC50242.1"
                     /translation="MKIFSGTSNPPLAKAICKYVGIPLGRSNIKPFPDGETFVQIEEN
                     VRGEDVYVVQSTSPPTNHHLMEMFIMIDALRRASAARITAVLPFYGYARQDRKDQPRV
                     PITAKLVANLLVAAGANRILTMDLHAQQIQGFFDIPVDHLYAAPVMYNYLKRKRLRNL
                     VVVSPDIGGLKMAYAYSRVLGAGLAIVAKRRKSAREVVSMAIIGEIRGKDVLLVDDLT
                     ETASTLVKAAELIKHRGAKRIRACVSHAILDDLALERLSKSAIDELVTTDTVFRPAIR
                     GVKIVTLSVAALLGEAIKRIHSNSSVTSLFAFKGGSTS"
     gene            6434..7201
                     /locus_tag="PKI07_03985"
     CDS             6434..7201
                     /locus_tag="PKI07_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOC50243.1"
                     /translation="MKSVLLNATPRVLRRRAGAKALRRSGQIPAVVYGRKTPPQSLEV
                     NARQLENLIHHSASENLLVDLVVQGDARPQRLALVQEIQHHPLTGKMLHVDFHEVAED
                     EKVTIQVPVETVGEAEGVKNEGGVLERVLFKIRVRGLPKDLPEQIVVDVSSLKVGQTI
                     HLGKIPAPPNTEILGDKQIPVIAVAAPRTEEEEAAEAAAAAETVLGEVEMIKEKKVEG
                     EEGAPPAKGAEKAAPAAEKAAPAAADKKAAPAAAKKK"
     gene            7250..7843
                     /gene="pth"
                     /locus_tag="PKI07_03990"
     CDS             7250..7843
                     /gene="pth"
                     /locus_tag="PKI07_03990"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015135146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOC50244.1"
                     /translation="MENLYLIVGLGNPGADYARTRHNAGFQMVERLARRWGVGWAYEK
                     KFNARLAWAQRNAQRVLLCQPQTYMNASGEAVGAVLAFYRVPLARVLVAVDDADLPLG
                     EVRLRPGGGSGGHHGLESIAGHLGTSEYARQRIGIGRQSGAREITGHVLGRFSSTEAA
                     LLDKVLTVASDQAETWLDAGIQKAMNQFNGVVGDPGN"
     gene            7843..8169
                     /locus_tag="PKI07_03995"
     CDS             7843..8169
                     /locus_tag="PKI07_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOC50245.1"
                     /translation="MKNAEKRYEGLFILDMAGKEEGVKDAVDRISTEITTLGGKVETV
                     QKMDKKTFARVADKKMSAGFYVNVIFHGPPAILEQLKRRFASNDEVFRVLFTNAPEVK
                     PAAAAN"
     gene            8178..8627
                     /gene="ssb"
                     /locus_tag="PKI07_04000"
     CDS             8178..8627
                     /gene="ssb"
                     /locus_tag="PKI07_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOC50246.1"
                     /translation="MASFNKVILVGNLTRDPELRYTPKGVAIAKLGLAVNRVWKTETG
                     ETREEVTFVDIDAFGKQAETIGQYLKKGRPILIEGRLRLDQWDDKQTGQKRSRLGVVL
                     ESFQFIDSNRSESGAEPARRGTAVPADAASSAPAPEPGPAEEDDVPF"
     gene            8691..9329
                     /gene="rplI"
                     /locus_tag="PKI07_04005"
     CDS             8691..9329
                     /gene="rplI"
                     /locus_tag="PKI07_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOC50247.1"
                     /translation="MAKTEVILTHQIVGLGAESDQVKVAAGYARNYLFPQGLAVPLSA
                     ANKRRIEALRARRAEREAHELNTMNELAKSVSKLLCLLTVKTGEDGKMFGTVTSGMIA
                     DQLKTQFDILLDKKQIHLETPIRALGDHEVELRLHADVTTKLKVRVESSTPLPVPEAP
                     APAEAGKEEHPRTEKRGRRPEAAHAKGEAGGKKPHGPRPGRPDKASKAEKAE"
     gene            complement(9414..11381)
                     /locus_tag="PKI07_04010"
     CDS             complement(9414..11381)
                     /locus_tag="PKI07_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC50248.1"
                     /translation="MTFANPQILWLLLALPPLLLAFFWWAGRKRRLLLTRFIQARLLP
                     DLTVGISPTRERIRAGCLVLAVACLLLALARPQWGFDWEEVKLRGLDIVVAIDTSKSM
                     LATDVAPNRLARAKLAALDLMQRARTDRLGLVAFAGSAFLQCPLTIDDAAFRQSVEAL
                     DVNIIPQGGTALAEAIQTALTAYKEGDNYKVLVLLTDGEDHDSDALAAAEKAAQAGLR
                     IFTVGIGTSDGEILRVQNPQGGSDYVRDEQGNVVKSHLNEDLLKRIAFATEGGAYIPL
                     RGAKAIDSLYDQRLAGLPKSQHQEKFIRRYHERYYWPLAAGIALLLAEMFFPERRRER
                     SAPRSRDATGGRRRTPDAPSPSGPRTVSVLLALLFFPSLLLHPWAAPASPSSALREYK
                     AGNYDQALREYERLLRQNTNDSRLQFNAGAAAYRNRQFAEAARRFNAALASPDLKLQG
                     SAYYNEGNSLYHLGERASDPQQRRQSWEKALTDYQSALKLNPQDADAKFNYEFVKQKL
                     EELQQQPQSSKSDSSDQKQDSNQNQDQQPQPNQDSSQPDQQKQPDQATPPPDQTDSKQ
                     DSSPPQPEEQQQTEQKPSPADQPQSAQQDSAPSGEKPDEADQQAAAQPLNQMTLEQAQ
                     QLLDAQKEDEKMLQPRPTGKPHRSGRPVKDW"
     gene            complement(11378..12376)
                     /locus_tag="PKI07_04015"
     CDS             complement(11378..12376)
                     /locus_tag="PKI07_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC50249.1"
                     /translation="MTFGHPYFLLLLLLLPALGWLKGRQGKPPAFIYSSVQLVRGILN
                     VSRTRSGAFLAALRWLVLALLILALAQPRLIRSETKVTASGVDIAVALDLSGSMASED
                     FEMKHERLSRLAMAKEVLRKFIAKRPADRIGIVAFATQAYIASPLTLDHDFLLENLAR
                     LELGIIDDSRTAIGSALSAAVNRLRELKSKSKIVILMTDGQNNSGKVAPLTVAEAARK
                     LGVKVYTIGVGTRGMAPMPVYWGGRKVGYRQEPVNIDEDTLQQIADLTGGKYYRADNS
                     QRFQAIYAEIDKLEKTEAEVKKYSHYDELLAWFLVPGLGLLLLEVLLRHTVWRRLP"
     gene            complement(12373..12948)
                     /locus_tag="PKI07_04020"
     CDS             complement(12373..12948)
                     /locus_tag="PKI07_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50250.1"
                     /translation="MTATNLHPSALAAAHTGTNALRDIKPPVEIPSGWAWVLWGLLAL
                     AIAALAFWAWRYWQKRRAQARIIPAVPPHLRAKQKLREALALLGQPREFCILVSDTIR
                     GYLEERFDFRAPERTTEEFLHELRGTPLLTPDQKDSLGEFLQRCDLVKFAKYEPSPPE
                     LEGLHASALRLVEETEPVMATLEAVKSGAPA"
     gene            complement(13052..14008)
                     /locus_tag="PKI07_04025"
     CDS             complement(13052..14008)
                     /locus_tag="PKI07_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOC50251.1"
                     /translation="MIPREILKKIRQIEIRTNRLVSETLAGQYHSVFKGQGMNFDEVR
                     EYQPGDEVRFIDWNVTARMNHPFVKKFVEERELTLMLVVDASGSGRFGSHAQSKRELA
                     AEIASVLAFSAIRNNDKVGLILFTDGVEKFIPPRKGRRHVLRVIREVLFFEPRHRGTD
                     LNGALEFLLRVTPHRAIAVLISDFIGSPVEVSGRRRRKVRPQMMLLESLAQASLTMLR
                     QANRRHDVVAVQIMDRYELELPALGRLVLNDAETGEVVEINTSDERKRAAFAERQARA
                     QADLARLFRSAGIDAIRLRTDQPYAAELGRFFETREKRRLRG"
     gene            complement(14094..15083)
                     /locus_tag="PKI07_04030"
     CDS             complement(14094..15083)
                     /locus_tag="PKI07_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOC50252.1"
                     /translation="MNTGINAINAAVQEAGAFVRPLFNELGKVIVGQTYLVERLVIGL
                     LANGHVLLEGVPGLAKTLAVKSLANCLNVKFARLQFTPDMLPADVVGTQIYNPQSGAF
                     TTRKGPIFANLVLADEINRAPAKVQSALLEAMQEKQVTIGDHTFKLEEPFLVLATQNP
                     IEQEGTYPLPEAQVDRFMLKLKIGYPSRAEERQILELMARTSGTPVTGRVVEPRQILH
                     ARQVLNDIYMDDKVKDYIVDLVCATRDPEAHKIQVREFIQLGASPRATIYLTLAAKAY
                     AFLKGRGYVTPQDVKSIGMDVLRHRVAITYEAEAEEKTSETIIQKIFDELPVP"
     gene            15292..17817
                     /locus_tag="PKI07_04035"
     CDS             15292..17817
                     /locus_tag="PKI07_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOC50253.1"
                     /translation="MKLPFSVPVRFRSALPAFLGLLLLRASGPAVQAAPASAEQAAAA
                     VAGWLDLSATPFGEALGRSVRQVDTASDKAGNPLYHIVSLAPAGFVLVAADDLVEPII
                     GFAAAGKFVPSEDNPLGALASRDLAGRMARARKAGPAVPGSAAAQAQAKWQQLCARAD
                     DAAATPKGLIVVSDLRVAPLLQSTWDQETAAGVGLAACYNYYTPPYGPGRTTNYPSGC
                     VATAMAQFLRYYQYPDTGVGTASFTISVRGVPQAYSLRGGNGSGGPYLWSDMPLAPPA
                     TPTTAQCQAIGALVADAGATVNTDYAANGSAASVLDVKGALLGTFKFSHAAQGWKNNK
                     NIGESLTRMINPNLDARGPVLLGIDGPNGGHAVVVDGYGYHLATLYHHLNLGWSGGAN
                     AWYALPFIDAAVYTFTTITKCIYNAYTNGTGEIISGRVLDQRARPVANATVSAVRTGG
                     GTYTATTDTNGIYALARIPASSTYTLTVSKADFSSAVSNFTTGVSVNLTATCGNYWGA
                     NFTLNQLPTVLDRLEWSAIGATQSVGAPLSATLTALNADNGIVTGFAGPVALSACSPG
                     ALATNTLLGNLGSPQSQTDPNYAWTQGYEFTPATNLLAFAVRSYSGTKITLWTGAGAF
                     IASRSVTNPPGTWTETALDAPVLLSAGTPYRLAALFPAGVPRYYTVYTGTWPTTFAHG
                     TIGQDCYYIYEEGFPNQVLGSGYGLFLDLRYTVPYTNDIAITPPVSGSFVNGVWNGNI
                     TVETAAAGVVLKADDGAGHIGLSNPFNVIAPIQLRFPRITAGGQFQCTVSSAPSLKLE
                     ILGSTNLLNWAPVATLTNTSGAFPFTDPAALRAARFYRARQLN"
     gene            18043..18831
                     /locus_tag="PKI07_04040"
     CDS             18043..18831
                     /locus_tag="PKI07_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50254.1"
                     /translation="MAAGGAAAAFGQDAVYAARVASQSDTNWDFPFYWINVGDYTPYN
                     PSTEQSSATQPVSTPSRIGSYVHTARLMSVGGGFGLVHIAGTNANAIYEVQITQPGKG
                     VAPDIIMNVGSTNGDLGGVFGATAGGGWTNTTAFQRNYSSNQWAFTCYLTNRPRVTQP
                     HIDFKYVSGGTGSDRTYADCVRFHQIGIRTNVPSPVRITRLAGASLDYTGGAGARFVL
                     LQSPSLPSAPANWQRLATNTATPGSFSIPPAGAAAAAFYCIQAE"
     gene            18991..22140
                     /locus_tag="PKI07_04045"
     CDS             18991..22140
                     /locus_tag="PKI07_04045"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOC50255.1"
                     /translation="MRRSLSFSRGRTSPAPWGRRFRWLAVALLLYTLAGFFLLPALLR
                     WQLVKRLPGLTHRQAAVQKVKFNPYALTLSIQGLALTETNGTPFAGWDELFVNFQLSS
                     LFHRAWTFREIRLERPTATILRAASGQCNFANLGRPAPASAPAADEIPKPPPAVRIGH
                     LIVTNGVVTFADQTPARPMRLEIHPLNLDLRNLATRRDEKGQYALTLRSADGGHWDWA
                     GTLALNPPRSAGTFTVRGARLAVGAPYLAELTRAEIADGRLDASGEYRLAAEPALQLE
                     ITHATVKIEHLQLTDPGAGETLLALNQFDAGDASFHLAGRQVRLPRVVARGGAALLRR
                     ADDGQWNWARQLVARPPAPPASAAPAAAPAETRAPWEVAVAEFRLEDFAVVAEDHVPA
                     PPARLGLDGLRIRATGFSTRPNAPLAATVEGKWRGGGTVRIEADGTVQPPAGTAKIAL
                     AGLALPPLQPYLARQLRLALQSGELSLNGQARYAPGAAPAPRARFAGDLALAKFAVAE
                     TPGGRELAKWDELALRGIELAWHTNALNVDEARFQGLKASLVVNSNGQLNVTALAPAP
                     PAAAPARAPATPPAAAAAPAPAAFPLRIGTLAFAQCSLQAADESLAPPVHARLEEFSG
                     TIRNLALPATGPAGVDLRGKISALAPFEITGDITPEAKNLQVDLKLGLKNSDLAPLAP
                     YSEKYAGHPLHRGKLSCDAAYKVANRQLDARHTIVLDGLALGARNPAANAPNLPLKLA
                     VALLKDRNGRITLDLPVSGNLDDPKFKLGAILGKTLSNLLLKVAASPFALLGAITGGG
                     GEDLQYVDFPAGAATLAGPQTNKLARLARALHERPALGLEISASANAVTDREALTRQK
                     LLARLKLARAEELAAAGQALPPPDELQLEPADYDRLLRKVCEAASPAAPENLPVLEDW
                     AAAAAAPPAGPDAGAPAAPRSAPATEKGALWLLRRLFSPSPPPPAPAAAPETADTPAA
                     APPAAAGLGRAEMEQRLMRALAATDDDLRELIRQRAQAVRQYLRDTGKVPADRLFLAP
                     PQPVPPGTNGLARATFSLG"
     gene            22277..23371
                     /locus_tag="PKI07_04050"
     CDS             22277..23371
                     /locus_tag="PKI07_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC50256.1"
                     /translation="MNDDILPRLPEPKPNVDEFRRVLQGGDCGRVPLVELAIAEEVLA
                     ALHGRPLIPLSRAEGWQTFRAGVRQRVRLHRALGYDYYRVRAEIPFQMETLPARNTAA
                     AAAADRQWVNEQEGLIKTAADLETYPWPTRAAIDFAAAEAAADGLPEGMGLIGFSGGV
                     LEWSSNLLGLESMMLLLYDEPGLVQAVVDRVGQIIYEAFHVFCQMDGVFAIWLGDDMG
                     FKTATLLQPRHLRQYILPWHQRYAELAHRTGRLFLLHSCGHVEAIMPDLIETVRIDAK
                     HSFEDVIVPVEEFKRRWGTRVAVLGGVDVDLLSRGPEAAIRRRTGQILAACAPGGGYA
                     CGSGNSITNYMPAANYVAMLETLHRFNGRL"
     gene            complement(23436..24347)
                     /locus_tag="PKI07_04055"
     CDS             complement(23436..24347)
                     /locus_tag="PKI07_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC50257.1"
                     /translation="MRNLRAFWFLLIGLWAVGAGWGCRCGGGPGAAADKTFVLWQLPN
                     QTHSQIMSYVLRTAGGKVIVLDGGTTGDAAYLKGFLAALGNEVEAWFITHAHDDHFGA
                     LSQLLEQGAAPRIRAIYGSLPTQEWVNAHGVGAEDTRPYEQFQQALRKANQVIRDLAL
                     GQEMTIDGVRFEVLGVHNPEITVNAINNSSLVLRVSDAAKSVLFTADLGVEGGRKLLS
                     SPRAARLPSDYVQMAHHGQDGVEEAFYQAVQPARCLWPTPLWLWDNDSGQGKGSGPWK
                     TLEVRAWMEKMNIQEHYVMARGLFRIE"
     gene            complement(25436..26593)
                     /locus_tag="PKI07_04060"
     CDS             complement(25436..26593)
                     /locus_tag="PKI07_04060"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOC50258.1"
                     /translation="MISPGTTPASTTSTSTNTSATNKPPAGPPPPRLSRAAFLRLLGA
                     GVAAASLNLARSGPPGERPVARIQPIRGSWISIWWDDRRHLYWNDTCRNYTAQQWRQA
                     VREVAELGMEYLVLLAIAKGGQAFYTTPLLPKLELACADPLEALLAAADKYGVKFFLS
                     SDWFGPWDHRCLHDPARVRARFQMMSEIARQYSHHRSFHGWYWPNEAALGPYFTDEFI
                     AYVNACSREARRLTPRARTLIAPYGTHRAVNDDRFVRQLEQLEVDIIAYQDEVGCRRM
                     NPSQSAAAFEKLRQAHHRVPQRALWADVETFAWEGPPNQQSSPLIPAPFSRVKEQLAA
                     VSPFVDTILIYQYQGLMNKPGSKAFAGHPDSTQLYTDYRRWWRKHARKTPP"
     gene            complement(26526..27998)
                     /locus_tag="PKI07_04065"
     CDS             complement(26526..27998)
                     /locus_tag="PKI07_04065"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOC50259.1"
                     /translation="MYLVQIASECAPVAKVGGLGDVVFGLSRELEIRGNSVEIILPKY
                     DCLRYDQIYGLQLTFQDLWVPWHNGAIHCSVYFGFVHGRKCFFLEPHSEHHFFHRRVF
                     YGQHDDDQRFAFFCKAALEFMLQSGKHPDVIHCHDWQTGLVPVLLYEIYQHAGMRHPR
                     VCYTLHNLQHQGLTGEHILRATGLNRHAHFFHPDRLQDNFNHAALNLMKGGIVYANFV
                     TTVSPRYAWEILNTSHNFGLGHTLYVHRAKLGGILNGVDYEVWNPEIDRHLPRPYSLN
                     HLDAKYENKHALRQRFWLQDAYKPIVCYIGRLDHQKGVPLITHALHYCLQHGTQFVLL
                     GTSPNGGIGHHFWRLKQLYNDNPDCHLELSFNEELSHLVYAGAEMIIVPSLFEPCGLT
                     QMIALKYGTVPIVRATGGLADTVFDANYADRPYHERNGFVFNDYNNTGIESALHRAIG
                     LWYAYPQYFRELTLNGMRYDFSWNHPGQHYLNIYEYIRDK"
     gene            complement(28006..29205)
                     /locus_tag="PKI07_04070"
     CDS             complement(28006..29205)
                     /locus_tag="PKI07_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57"
                     /protein_id="HOC50260.1"
                     /translation="MAEIYHALGLHMHQPPGNLVALHNSAESWEARQILWCYDRPTRM
                     LEGYEDVARLHLSFSGTLLKQLEDPGVRQTFAEVADIEDFLNRYRRANIEYLGSGLYH
                     PVYPLTPQADWDAQTEWWQGLGRHLLGRDRFPGFWPPEMGFCMEMIPMLARHGYQYVL
                     VDSIYLKPRRPMRWEELRYRPYRARYEGAEIIVVPRDRELSNAQLSGTDPGWFQQEIH
                     ERTRPCDFPALVTTWTDGENGGWFRTTKIESGFWGFFYRPLLDRFRAGTLGFTPIHIS
                     QYLAQYPPTEEVDVHPGAWNTEHQWGGDFTQWTGSLLQKKGWDEVRRASEYYWQVKRA
                     FDQQLAALTHPEEIRRLIVRAFDHLLAAETSCNFYWGSRWVSRSFDELEQAYALLDAA
                     MHQLRNA"
     gene            29373..30710
                     /locus_tag="PKI07_04075"
     CDS             29373..30710
                     /locus_tag="PKI07_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50261.1"
                     /translation="MTPVSRRTFLGQSALTAGLAPLALSSLTSSVFVRSARAGEPGPN
                     GKVRLGIIGCGGMGQGDLACFLQNPEVDCVMVCDVDDARIAKGVEICQQKRGRKPDTA
                     RDFRRVLDRKDVDAVLIATPDHWHALPTVLACQAGKDVYVEKPLATTIDEGRAMLEAA
                     RRHQRVVQMGSQWRSCNHIVEAGEFIRSGKLGKVSIVRGWAYLDWLPSIGKPADCPPP
                     PGVDYDLWLGPAPERPFNPNRFHFNFRWFWDYAGGLMTDWGVHLINMMLMGMNREAPR
                     SAYSCGGKFVLDDNSETPDSQVTVYEFPGFTLIWEHKAGLDNGLYNRSWGVEWSGTEG
                     NIILNDQGWEIRTEKKRANLDNQRKPGSPDPRPAHVRDFLDCMKSRRQPVLNLDVGHH
                     VSTVAHLGNIAYRVGRKIVWDAAAERVEGDAQADKLVGVRYRKPWKLPYAKRA"
     gene            complement(30803..31945)
                     /gene="rho"
                     /locus_tag="PKI07_04080"
     CDS             complement(30803..31945)
                     /gene="rho"
                     /locus_tag="PKI07_04080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOC50262.1"
                     /translation="MSTAANTDNANTAATTEGAGYLEISEKGFGFLRTAENHFHPKPS
                     DIFVTPDTIKRSFLREGCLVAGPTQPPHRGNSPQLKSVDKVNGVPFSDYTKTVRFENL
                     TTIDPIEKFRLETTPDLIETRVIDMVTPLGKGTRGLIVAPPRTGKTTILKQIANAITA
                     NHPEVHVLVLLIDERPEEVTDFQRSVKAEVVASSNDQDLETHVRLSRFMIERCRRMVE
                     SGKDVFVLLDSITRVARAYNSAHGGSGRTMTGGVDARALEIPRKMFASARKIEEGGSL
                     TILATALVDTGSRMDELIFQEFKGTGNMELILDRKLSDRRLFPAIDIPKSGTRKEEKL
                     FPKHQIEAVRKLRRTMVDLNPVEAMETLVAALRKHKTNDDLLSKLL"
     gene            complement(32133..33116)
                     /gene="cysK"
                     /locus_tag="PKI07_04085"
     CDS             complement(32133..33116)
                     /gene="cysK"
                     /locus_tag="PKI07_04085"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOC50263.1"
                     /translation="MSRIYNNIVETVGRTPLVRLNKVTAEAPATILLKCEFFNPLGSV
                     KDRIGQAMIEDAERRGVLTKDTVIIEPTSGNTGIALAFVAAAKGYRLILTMPETMSQE
                     RRTLLAMLGAKLVLTPGAEGMKGAIAKAAQLARETPHAWMPQQFENPANPEVHRKTTA
                     EEIWADTDGKVDIVVAAVGTGGTLTGCCEVLKARKPSLQAIAVEPKDSPVISQTLAGQ
                     PLKPGPHKIQGAGAGFIPGNLHLKDARGNPQIVECIQVSNEDAFAMARRLAREEGILA
                     GISSGANVWAAIQVARRPENRGKLIVTIANSTGERYLSTALADEARAQLGG"
     gene            34731..35261
                     /locus_tag="PKI07_04090"
     CDS             34731..35261
                     /locus_tag="PKI07_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50264.1"
                     /translation="MKTRWERFNIYLLLAAAVAAVVLCGCQSAAPSKPGKLLAVLRLH
                     LETTPDGTNTLNHIPVYRLNPVQICAQKHAFLDEGHVAEAKVIEVVGGFALWIKFNSA
                     GTALFEEYTTANRGRRIAVFSQFGERIKDSRWLAAPIISRRVTDGIFTFTPDATRSEA
                     EEIALGLNNVARKVQK"
     gene            35382..36263
                     /locus_tag="PKI07_04095"
     CDS             35382..36263
                     /locus_tag="PKI07_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50265.1"
                     /translation="MKPQVDFEWRGGPAANARRRAARGARGSAFTLIELLVVIAIIAL
                     LAALLLPALARAKDKAWTVGCMNNLKQLALCWHLYATDNADLLAPNNSIMVFSGPSWS
                     VGAASISWCPDSPRTDTNTLALQSGVLFPYNTSVGIYHCPADQSKVESAGLPTAQRRN
                     RSYNMSQSVNGFPEYLEQLGIPGLSDIPSWRKLTEIRRPVPSRLFVFIDEHEDTLYDA
                     QFGNPAGAPWWGPMWFDMPADRHNRGGCLTFADGHVERWRWKVAKTGAYPGKVPDVGE
                     MPDYHRIQDAMKCWKDK"
     gene            36363..37538
                     /locus_tag="PKI07_04100"
     CDS             36363..37538
                     /locus_tag="PKI07_04100"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOC50266.1"
                     /translation="MNYNISRQAAALSPSLTLAIDAKAKAMKAAGEDVVGFGAGEPDF
                     DTPAHIKEACARALADGFTKYTPAAGLPELRAAIADKHQRENGLAFKPSQIIVSCGGK
                     HSCFNVIFATCQEGDEVIIPAPYWLSYPEMVKMAGARPVIVPTTDRTEFKLTADALRA
                     AITPRTRLFILNSPSNPTGSVYTPDEIKALGDVCLEHRVLIMSDEIYEHLLYDGAVAR
                     SVAGCSPAHAAQTIIVHGFAKAWSMTGWRLGWCAAPEPIARAIDAVQSHSTSNPTSFA
                     QKGAVAALTGPQDHLQTWLAEFDRRRTRAWNALNRIPGLSCVKAKGAFYLFPNIAGTG
                     LKSTDFCARLLEAEKVAAVPGIAFGADDYIRLSYATSMANIEKGLERIERFCRSLAR"
     gene            complement(37643..37942)
                     /locus_tag="PKI07_04105"
     CDS             complement(37643..37942)
                     /locus_tag="PKI07_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50267.1"
                     /translation="MNAPDPSLPPTREIATRLLPQCHCHQPRPSNWPVHAGANWLVVK
                     DTETAPNEPPLIEYECLNCHRRYRLRAAKLFEVLGDGTERAYMREGRYGRWLSCR"
BASE COUNT         6761 a        12316 c        12826 g         7204 t
ORIGIN      
        1 atcgccccgg agggcgactc ggcttcccgc cactacttgg gttggaagtc ccagccaccc
       61 ccaccccgag ggttttgagg aaggctggcg ggcgggaggt cttcgcgcaa gggcaatttt
      121 ttggggttgg tggtggcgtg agcggctggc cagttcttcg ggttcgcctt tttccctgag
      181 ggcatcagca ggccagcgaa gcggtaaaaa ctgccgccaa aaagcagatt gaagccaggc
      241 ccaattcttg ataagcttat tccatgctcg gccagtcctg taaaccagtt ctcccgtcct
      301 cgcgttgcgc ccaatccgga agggcgcgca tagaggcggg ttctgccagc tttggtggat
      361 gattgagcgc gattttgatg tgcccttcgt ggctgacctg gctttgcggg aaaagcagat
      421 ccagcagaac taccgtccag tcgtggcggt tcacaagtgg tttgcgcgcc gacctggcac
      481 actctttcgc gcgctcctcc tgtcggaatt tttagaaggg ccgcttcgcg agcagtttta
      541 ccgcgggcaa tcgcttaagc atctccgcgt gctcgatcct tttatgggtg ggggcaccac
      601 cctgctggag gccaaccggg ttggttgctc ggtgaccggc tttgatatta accccatggc
      661 ctggtggatc gtgcgccagg aaatcggcga gctgaatccc cgggcctacc gagtggcggc
      721 ggatacgctg cgggacacgt tggaaagcga gattggccat ctgtatcgga cccagtgccg
      781 ggagtgcggg gcccgggatg ctcacgtcaa atacttcctg tgggtcaagt caccgccgtg
      841 cgccaagtgc cgccaacctg tggatctgtt tccgaactat cttgttgccg cggatgttcg
      901 gcatcccacc aacctctttg tttgccgcga ctgcggcgag ctctatgaca gccccgcccg
      961 caagaacccg ccggggtgtc ctcactgcaa gacccggttt agcgtcgagt tcaccgcccg
     1021 ccgcaaccgc tgcaagtgca agcattgtgg ccacgtcaat gcctacccaa ctcccgaggg
     1081 cggaccgcct ccccatcgca tgttcgccat tgagtaccat tgcgagcgtt gccgtccggc
     1141 gcactcgggg cgcttcttca agaagcccgc tccggccgac ctccgccgtt acgacgaagc
     1201 cgccgcccgc ctggctgccg gcgaggcccg gtttattccc caggaggaaa ttcctcccgg
     1261 tgatgaaacc gaccggctcc atcgctgggg ctaccgattc taccgggaga tgtttaatgc
     1321 gcggcagctt ctggggctcg aactcctctg ccatgccatc aagcggcaga aagactcccg
     1381 aatagccgac gcactggcga cgaacctgtc tgacctggtt cgctatcaga acctgctctg
     1441 ccggtacgac acaaaggcgc tcaagtcgct tgatgtcttc tccgtgcacg ggtttccggt
     1501 cggcttgatc cagtgtgaat ccaacgtgct gggtatttcc gggaaagcga tggcagtggg
     1561 cagcggcggc tggcttaaca tcgttgacaa attcgccaag gccaaagcgt actgccgcca
     1621 cccgtttgaa atccggcatg aaggcaggcg aaaggttcgg gtgcccatcc tcaccgagtg
     1681 gatcggcgac cagaggaacg accttcaccc tccggagaca aaggaagtcg ccctggcgtg
     1741 tgccgatgcc tccgaggctg attttaccgg ccgcaagttc gacgctatcc tgaccgaccc
     1801 tccgtacttc cagaacgtcc agtacgcgga gctaatggac ttttgctacg tctggctgcg
     1861 taaactggtc aaggctgccc atcctgaatt cgacggtctt acgacgcgcc acaccaacga
     1921 actgaccggc aacgtcagta tggggcgcac cctggagcat ttcacggaag gcatatcccg
     1981 ggtgtttcgc cgcatggcaa aagccctgaa tcccggcgct ccattggcct tcacctacca
     2041 tcacaacagc ctcgacgcgt acctccccgt agcggtcgcc atgctcgact ccgggctcgc
     2101 ctgctccgct tcgctgccct gtccggccga gatgggggct tccattcata tcaatgggac
     2161 cgtatcttcc atagtggata ccgttttcgt ttgccgttct acgggcaaat tcccccgccg
     2221 ctggctggcg gacactcccg cggctctcgc ccgcgttgcc gccggggatc tcaggttgtt
     2281 ggagcaaggg caaatgaagc ctacccaggg cgacatgcgc tgcgtcgtct ttgggcatct
     2341 gatccgcctg gccgtatggc gcctgcgccc gacttgggtt gctacgccca aggcggcgga
     2401 tcgtatggcc gccgtacggc gttggattat caagtttggt ggggccgatg ccgttctgca
     2461 agaactgggg gatgcttatg ccagggccag ggctgaacaa caatgggaat tgaacgaaga
     2521 agataaagcc tttcgaacct cgacagatga aatatccttt tgaggccgat taccaggaaa
     2581 tcctcgccga ttccgaccgc ttcgttacgg ccattgtcac cagtctcgcc tctgagttcc
     2641 ttacgcttcc caagggtaaa ggcttcatcg aatatcccca gttcgcagca ggctacgagg
     2701 atttgaagaa ggtcacgacg ggcttttcca aactgcctcg gcgggaaatc gtggacttgg
     2761 ttgcgtcaac gccggtctgc ttcatagtga tccgctccat tctcggcttc acgccttccg
     2821 aatgggcgta cgtggccagc cagcgcaggg gcgccgcggt gacgcaaggc tttgcccgca
     2881 ctctcgaccg caatatccgg atgcagccgc tcaagccctt gaaatgccgc ggtctgaccc
     2941 gggagcgcct gaccgccctg ctggacgcgg cctgcgatct tttgcaagag ggctgtccga
     3001 aacccaatga catcacgatc catcgcctcc agaaggccga caccaaagag gggcttcaat
     3061 ccatcaagtc actcgcccgc atgggggccc cctacgccat gctcctttac gaacgatttc
     3121 tcggccgtcc ctttgccggc catagagact ccgtgagcga attggtcgga gacagcctgg
     3181 agtccgccat tgaagacgtt ctgagcaagg ccggtgtcag ctatcgaaag accaagcgcg
     3241 ccgaaagggt gcccggcttc gatcaggcgc ctgatttcat cgttcccgac gagtttactc
     3301 cccgtgtggt catcgaggcc aaaatcaccg aagatgacgg caccgcgcgc gacaaggtga
     3361 cccgcatcca gcatttgggc gagctcagca cggccggcgc accggtcggc cagccgaaat
     3421 atcaagtcat tgcgtgcatc gccggcaggg ggtttggcgt ccggcgcgag gacatgagga
     3481 agatgctctt tgccacccgg ggcaaggttt ttacactcaa aacgctcaac cggctggtcg
     3541 agtgttccga tctcgcaaaa ttcgccacaa agccttacta atcaaccaac gccttgaatc
     3601 cgttatgacc tttgcccgcc tcgatctttg caaatacctg cattccaagt gctcccgcgg
     3661 gaaccgcggt gcgcgtgata gcgttgctgg cttccgcgcc caggcggcct ccgcttggga
     3721 gggccgctcc ggccgcgatg ttggctggtg gaggggcggc aagatgcgcc tcctttcccc
     3781 gggattcgga acattcgcga gcggagtgcg taagaaatta cctgtgcagc gtccggttat
     3841 cagcgggcgc ggcaaaggcg gttttggggc gagggttatg aggcttggaa ttgcagtgcg
     3901 gctggggggg atcggggggt tgctggcggt ttggtgcgcc tcggccacga ttacgctgga
     3961 gccgtgggcc ccgatgtttc ggggggtgga ttttgcgcgg ggggaggcgg atgccaacga
     4021 ggcgcggttg cagaaggtgt ttgcgctgcg ggtggatttg cacgatccga cagtggagtt
     4081 tttttcgacg cccgcgaacg gtgaccggcc gaaggagacc gacggccaga cgacggctgc
     4141 gttcgtgcag agttatgggg tttcggtggg agtgaatgcg aacttttttt cgcccgtcaa
     4201 ttacaccccg aatgatccgc gcgatttaaa cgggctggcg gtttcccggg gggaggtggt
     4261 gtcggcattt gagaacggat ttccggcggt gatgatcacc cggagcaatg cggtcggttt
     4321 tgtgtctcag gcaccgggga gcctggcgaa tgtttggacg gcggtggcgg ggtcggacct
     4381 gattttggtc aatggacagg caaagatggc ggattgcaat actgaattct gcaagccgca
     4441 tccgcggacg gcggtggggt tgtcggcgca gaggcggtat ttttacctga tggtgattga
     4501 tgggcggcgg ccgggctgga gtgacggagc aacgctgtat gagacggggc agtggttgtt
     4561 gcgattgggg gcctggaatg ggttgaatct ggacgggggc gggtcgtcag cgatggcgcg
     4621 gttgaccaat ggcgcggcgg tggtgatgaa tcgccccagc gactggctgc cgcgggtgaa
     4681 tgggaatcac ctgggggtgt ttgcgcagcc gctggcgccg gcgttgcttg cccaaccgac
     4741 caaccggacg gtgtcggtgg ggcaaccggc cactttccat gtgggggtgg ttggcgcgcc
     4801 gccgctgtat tttcagtggc gcttcaacgg ggtcagtctg acgggggcga ccgaggcggc
     4861 gtgcacgatt ggcagcgcgc aaatggccca cggcggatgg tactcggtgg tggtgtccaa
     4921 tgcctggggg gcggtggtga gttccaatgc gttgttgtcg gtgcaggagg aggggtcgct
     4981 ggtcggccgg ctgtttggcg cgcaaaagga cggggaggtt ttttcggtgt ggctgcaggc
     5041 cgacacggtc gggaaataca gcctggagtg gcgggacctg ccggcggaga gcaactggtc
     5101 cgcgtggccg gccgtaaatg ggaatggcgc cgcgctgcaa ttggtggatc cgcaggcgac
     5161 caatgccctc cggctttacc gggtgcggcg gtggtagcgc cgcttgcggg ggcgggcggg
     5221 aggcggattc attttccggc cgccggggag ctggccgttt gcgaggctgt ccgcccggtt
     5281 ggggccgggc ggcccacggg aggccggccc cggagcggcg ccctttttcc agcggcgggg
     5341 cagggggcgg gaattccggc ttgcgcgcgg gctggggttt ggtaaagtgg ccccggtttt
     5401 ttccaccgga aggtggttgg ttccgtatcg aagaatagac agctagatat tgtgtgaaga
     5461 ttttcagcgg cacttcaaat ccgcctttgg ccaaggccat ttgcaaatac gtggggattc
     5521 ccctgggccg gtcgaatatc aagccctttc cggacggaga gacgtttgtc cagattgagg
     5581 agaatgtgcg cggggaggat gtgtatgtgg tccagtccac cagcccgccg acgaaccatc
     5641 atttgatgga gatgttcatt atgatagacg cgctccggcg ggcgagtgcg gcgcgcatta
     5701 cggcggtgct gccgttttat ggttatgcgc ggcaggatcg caaggatcag ccgcgggtgc
     5761 ccattaccgc caagctggtg gcgaatcttt tggtggcggc gggggccaac cgcatcctga
     5821 cgatggactt gcacgcgcag caaatccagg ggttctttga tattccggtg gatcatctct
     5881 acgcggcgcc ggtgatgtat aattatttga agcggaagcg gctgcgcaac ctggtcgtgg
     5941 tcagcccgga tattgggggg ttgaagatgg cttatgcgta ttcgcgggtg ctgggggcgg
     6001 ggctggcgat agtggccaag cggcgcaaga gcgccagaga ggttgtgagc atggcgatca
     6061 tcggggagat tcgggggaag gacgtgttgc tggtggatga cctgacggaa acggcgagca
     6121 cgctggtgaa ggcggcggag ttgatcaagc accggggcgc caagcggatt cgggcctgtg
     6181 tttcgcatgc gattctggat gatttggccc tcgaaagatt aagcaaatcg gcgattgacg
     6241 aattggtgac aactgataca gtgtttcgcc ctgccattcg gggcgtgaaa atagtcacgc
     6301 tgtcggtggc ggcgctgttg ggcgaagcca tcaagcgaat tcacagtaat tcgtcggtta
     6361 cttcgctatt tgcatttaag ggcgggagca ccagctaggc gctggaaagc agccgtcgaa
     6421 cagtataaag acaatgaaat cagtattact caacgctact ccgcgcgttc tgcgccggcg
     6481 ggctggagcc aaggctctgc gccgcagcgg gcagattccc gcggttgttt atggccggaa
     6541 aaccccgccg cagagcctgg aggttaatgc ccggcagttg gaaaacctca ttcatcattc
     6601 ggcctccgag aacctgctgg tggacctggt ggttcagggt gatgcccggc cccagcggct
     6661 ggcgctggtg caggaaatcc agcatcaccc cctgacgggc aagatgctgc atgtggattt
     6721 tcatgaagtc gcggaggacg agaaggtcac cattcaagtg cccgtggaaa cggtcggcga
     6781 ggccgagggc gtgaagaatg agggcggcgt gctggagcgc gtgctgttca agatccgggt
     6841 gcggggtttg cccaaagatt tgccggagca gattgtcgtg gacgtcagct cgctcaaggt
     6901 cggccagacg attcacctgg gtaaaatccc ggcgccaccg aatacggaaa tccttggcga
     6961 caagcagatt cccgtcatcg ccgtggccgc cccgcgcact gaggaagagg aagcggccga
     7021 agcggcagcg gcggcggaga ctgtgctggg cgaggtcgag atgatcaaag agaagaaggt
     7081 cgaaggcgaa gagggcgcgc cacccgccaa aggcgcggag aaagcggccc cggccgccga
     7141 aaaggccgcc ccggcagccg ccgacaagaa agccgcgccc gcggccgcaa agaagaaata
     7201 gtatgggtgg cggcccggcg ggcgccgggc ggccaggccc gctccccgca tggagaacct
     7261 gtatctcatc gtgggtttgg gcaaccccgg cgcggattat gcgcggacgc ggcacaacgc
     7321 gggatttcaa atggtcgaac ggttggcccg gcgttggggc gtcggttggg cttatgaaaa
     7381 gaagttcaac gcgcggctgg cctgggcgca gcggaatgcg cagcgggtgc tgttatgcca
     7441 gccgcagacg tatatgaacg cgagcggcga agcggtgggc gcggtgctgg cgttctaccg
     7501 ggtgccgctg gcgcgggtgc tggtggcggt ggacgatgcg gatttgcctt tgggcgaggt
     7561 ccggctgcgg cccggtggcg gcagcggggg ccatcacggg ctggagtcca ttgcggggca
     7621 cctgggcacc agtgaatacg cgcggcagcg gatcgggata gggcggcaga gcggggcgcg
     7681 cgaaataacc ggccatgtgc tgggccgatt tagttcgacg gaagccgcgt tgctggataa
     7741 agttttaacc gtggcatccg accaggccga gacctggctg gatgcgggaa tacagaaagc
     7801 gatgaatcaa tttaacggag tcgtaggcga ccccggaaac taatgaaaaa cgcagagaaa
     7861 cgatacgaag gcctgttcat cctcgatatg gcgggcaaag aagaaggcgt caaagatgcc
     7921 gttgaccgga tttccaccga gatcaccacg ctgggcggca aagtggaaac cgtgcagaag
     7981 atggacaaga agacctttgc ccgcgtcgcg gacaagaaga tgagcgccgg cttctacgtg
     8041 aatgtcattt ttcatgggcc accggccatc ctcgagcagt tgaagcgccg gtttgcctcg
     8101 aatgacgagg tcttccgggt gttgttcacc aacgctcccg aggttaagcc ggccgcagcg
     8161 gccaactaag gacgtttatg gccagcttta acaaagttat cctggttggc aatttgacgc
     8221 gcgatccgga attgcgctac acccccaaag gggtggccat cgccaagctt ggcctggcgg
     8281 tgaaccgggt ctggaaaacc gagacgggcg aaaccaggga agaggtcacg tttgtggaca
     8341 ttgacgcgtt cggcaaacag gcggagacca tcgggcaata cctgaagaag gggcgtccga
     8401 tactcattga gggccggctt cgcctggatc agtgggatga caagcaaacg ggccagaagc
     8461 gcagccggct gggggtcgtg ctggagtcct tccagttcat tgacagcaat cgctccgagt
     8521 ccggcgcgga accggcgcgc cgcgggacgg ccgtcccggc ggatgccgca tcttccgcgc
     8581 ccgcaccgga acccggcccg gccgaagaag acgacgtgcc gttctagttt caacctgcgc
     8641 gagcgggatt gtcgttcgcg caaccagcaa cctgcaatac ctgatttcca atggctaaaa
     8701 ctgaagttat actgactcac caaatcgtcg gcctgggcgc tgaatccgac caggtcaaag
     8761 tcgccgccgg ttatgcccgc aattatctgt ttccgcaggg cctggccgtt cccttgagcg
     8821 ccgccaacaa gcgccgcatc gaggcgctcc gagcgcgccg cgccgagcgc gaggcgcacg
     8881 agttgaacac catgaatgag ctggccaaga gcgtttcgaa gctgctctgc ctcctcacgg
     8941 tcaagacggg cgaggacgga aagatgttcg gcacggttac gtcgggcatg attgccgacc
     9001 agctcaagac ccagtttgat atcctgctgg ataagaagca gatccacctg gaaactccca
     9061 tccgtgcatt gggtgaccac gaagtcgagt tgcggctgca tgccgacgtc accaccaagc
     9121 tgaaagtccg cgtcgaaagc agcacgccgt tgccggtgcc ggaggcgccc gcgccggccg
     9181 aagccgggaa agaagagcat ccccggaccg agaagcgcgg gcggcgtccc gaagccgctc
     9241 acgccaaggg cgaggccggc gggaagaagc ctcacggccc gcggcctgga cgcccggaca
     9301 aggcctcgaa agcggagaaa gcggagtagc ggttcgacgc ttccaaccgc ttgccgcggg
     9361 acattgtctg cggttaatgc gggccgggcc ggttggcgcg ctttccgcgt gggctaccag
     9421 tccttaaccg gccgacccga tctgtgcggt ttgccggtgg gccgcggctg gagcatcttt
     9481 tcatcctctt tttgtgcgtc cagcaactgc tgggcctgct ccagagtcat ctggtttagc
     9541 ggttgagcag cggcttgctg gtctgcttcg tccggttttt ctccggatgg agcggagtct
     9601 tgctgcgccg attgcggttg atcggccggc gaaggttttt gttccgtttg ttgctgctct
     9661 tccggttgcg gcggggagga atcctgcttc gagtctgttt ggtccggcgg gggcgttgct
     9721 tgatccggct gcttttgctg atccggttgc gatgagtcct ggtttggctg cggctgctgg
     9781 tcctggtttt gattcgagtc ttgtttttgg tcggacgagt ccgattttga cgattgcggc
     9841 tgctgctgga gttcctccag cttctgcttg acgaattcat agttgaattt cgcgtctgca
     9901 tcctgcgggt taagcttcaa ggcgctttga tagtccgtga gcgccttttc ccaggattgc
     9961 cggcgttgct gaggatcgga agcgcgctcg ccgaggtggt agagggaatt gccctcgttg
    10021 tagtaggccg atccctgcag tttcaggtcg ggcgaggcca gcgcggcgtt gaatcgccgg
    10081 gcggcttccg cgaattgccg gttgcggtac gcggcggcgc cggcattgaa ctggaggcgg
    10141 ctgtcattgg tgttctgccg gaggagccgt tcgtattccc ggagcgcctg gtcatagttc
    10201 ccggccttgt attcgcgcag cgcgctggaa ggggaggctg gggccgccca cggatgcagc
    10261 agcaggctgg ggaagaagag cagggccaga agcacagaga ctgtccgggg cccggagggg
    10321 gacggcgcgt ccggcgtgcg gcggcgtccg cctgtggcgt cccgcgagcg cggggcggag
    10381 cgttcacgcc tgcgttccgg gaagaacatt tcagccaaca ataacgcaat ccccgccgcc
    10441 agcggccaat aataccgttc gtgatagcgg cggatgaatt tctcttgatg ctgagatttg
    10501 ggcaggccgg ccaggcgctg atcgtacagt gagtcaatcg ccttggcccc gcgcaagggg
    10561 atgtaggcgc cgccctcggt ggcgaaggcg atccgcttga gcaggtcttc gttcaggtgc
    10621 gatttgacga cattgccctg ttcgtcgcgg acgtaatcac ttccgccctg ggggttctgg
    10681 acgcgcagaa tctccccgtc gctcgtgcca atacccaccg tgaaaatgcg caagccggct
    10741 tgcgcggcct tttccgccgc ggccagcgcg tccgaatcat ggtcctcgcc gtccgtgagc
    10801 agaacgagca ccttgtaatt gtcgccttct ttgtaagcgg tgagggcggt ttgaatcgcc
    10861 tccgccaggg ccgtgccgcc ctgggggatg atgttcacat caagggcctc cacgctttgg
    10921 cgaaaggcgg catcgtcaat ggtcagcggg cattgcagga aagcggagcc cgcgaaggcg
    10981 accaggccca ggcggtcggt ccgggcgcgc tgcatcaaat cgagcgcggc gagtttggcc
    11041 cgggcgaggc ggttgggcgc gacgtccgtc gccagcatgc ttttcgaggt atcaatcgcc
    11101 accacgatgt ccagcccgcg cagcttgact tcctcccagt cgaatcccca ctgcggccgc
    11161 gccagcgcca gcagcaggca ggctacggcc aaaacgaggc agccggcgcg aattcgctcg
    11221 cgggtggggg aaatgccgac ggtcaaatcc ggcagcaggc gcgcctggat gaatcgcgtg
    11281 agcaacagcc gccgtttccg gcccgcccac cagaagaagg ccagcagcag cggcggcaac
    11341 gccagcagca gccagaggat ctgagggttg gcaaacgtca tggcagtctc ctccagaccg
    11401 tgtggcgcaa aagaacctcc agcagcagca agcccaggcc cggcacgagg aaccaggcca
    11461 gcagctcgtc gtaatgcgag tattttttca cttccgcctc ggtcttttcc agcttgtcaa
    11521 tttcggcata gatcgcctga aaacgctgtg aattatccgc gcggtagtat ttgcctcccg
    11581 tcaggtcggc gatctgctgg agcgtatcct cgtcaatgtt caccggctcc tgccggtagc
    11641 caactttgcg cccgccccaa tagaccggca tgggcgccat gccccgggtg ccgacgccga
    11701 tggtatagac cttcacgccg agcttccgcg cggcttcggc gaccgtcaag ggggcgactt
    11761 tgccggaatt gttttgcccg tcggtcatca gaatcacaat tttactcttg gacttgagtt
    11821 cgcggaggcg gttgacggcg gcgctcaggg cagagccgat cgccgtgcgg ctgtcatcta
    11881 tgatgcccag ttcgagccgc gccaggtttt ccaggaggaa gtcgtggtcg agggtcaggg
    11941 gcgaagcgat ataggcctgg gtggcgaagg ccacgatgcc aatgcggtca gccggccgtt
    12001 tggcgataaa cttgcggagc acttccttgg ccattgccag ccggctgagg cgttcatgct
    12061 tcatttcgaa gtcttccgag gccatgctgc ccgacaggtc cagcgccacc gcgatgtcca
    12121 cgccgctggc cgtgaccttt gtttcgctcc ggatcaggcg cggctgggcc agcgcaagga
    12181 tcagcagggc cagaaccagc cagcgcaacg ccgccaggaa cgcccccgag cgcgtgcgcg
    12241 agacattgag gatgccgcgc accagttgga ccgacgaata gataaaggcc ggcggcttgc
    12301 cctgccggcc tttgagccag cccagcgccg gcaacagcaa cagcagcagc agaaaatatg
    12361 gatggccaaa ggtcatgcgg gcgcgccgct cttgacggcc tccagggtgg ccatgacggg
    12421 ttcggtttcc tccaccagac gcagcgcgga ggcgtgcagg ccctccagtt cgggcggact
    12481 gggctcgtat ttggcaaatt tcaccaggtc acagcgctgg agaaactcgc ccagactgtc
    12541 cttttggtcc ggcgtcagca acggtgttcc ccgcagctcg tggaggaact cctccgtggt
    12601 gcgttcgggc gcccggaagt cgaaccgttc ttccaggtac ccgcggatgg tgtcggacac
    12661 gagaatgcaa aactcgcggg gctggccgag gagggccagc gcctcgcgca gtttttgttt
    12721 cgcgcggaga tgcggcggca cggccgggat aatccgggcc tgcgcgcgcc gtttctgcca
    12781 atagcgccac gcccagaacg ccagcgcggc gatcgccagc gccagcagcc cccagaggac
    12841 ccaggcccag ccgctgggga tctccaccgg cggcttgatg tcgcgcagcg cgttggtgcc
    12901 ggtgtgggcg gccgccagcg cggagggatg gaggttggtg gcggtcatgg gcgctggggg
    12961 gcggagactg ggcccgcggt ggttcccgcg ccatacgcgc cccgtacggc gtgcccgccc
    13021 ggcttccctg tttgaacgcc tccgcgcata cttatcctcg cagcctccgt ttctctctcg
    13081 tctcgaaaaa ccggcccagc tcggccgcgt agggctggtc ggtgcgcagg cggatggcgt
    13141 caatgccggc ggaacgaaac agccgcgcca ggtccgcctg ggcgcgggcc tggcgttccg
    13201 caaaagcggc gcgcttgcgt tcgtcgctgg tgttgatttc gacgacctcg cccgtttcgg
    13261 cgtcgttgag caccagccgg cccagcgccg gcaattccag ttcataccgg tccataatct
    13321 ggacggcgac cacgtcatgg cgacggttgg cctggcgcag catggtcagg gaggcctggg
    13381 cgagcgattc cagcagcatc atctgggggc gcaccttgcg ccggcgccgt ccggacactt
    13441 ccaccggcga gccgatgaaa tcggaaatca gcaccgcaat ggcccggtgc ggcgtgacgc
    13501 gcagcagaaa ctccagcgcg ccgttcaaat cggttccgcg atgccgcggc tcaaagaaaa
    13561 gcacctcgcg aatcacgcgc aggacgtggc ggcggccttt gcgcggcggg atgaactttt
    13621 ccaccccgtc cgtaaacaga atcagcccca ccttgtcgtt gttgcggatg gccgagaagg
    13681 ccagcaccga ggcgatttcc gccgccagct cgcgtttgga ttgcgcgtgc gagccgaacc
    13741 ggcccgagcc gctggcgtcc accaccagca tgagcgtcag ctcgcgttcc tcgacgaact
    13801 ttttcacgaa cggatggttc atccgcgccg tgacgttcca gtctatgaag cgcacctcgt
    13861 ccccgggttg atactcgcgc acctcgtcaa agttcatgcc ctgccccttg aagacgctgt
    13921 ggtactggcc cgccagggtt tcgctcacca ggcggttggt gcgaatctca atctgccgga
    13981 tcttcttgag aatctcgcgc ggaatcatgt cagcagccag gctggcggcg ggggcgtccg
    14041 atgcagggcc aacgtctcac gacctcattc ctcgggcagg gttccgggct cgcctacggc
    14101 accggcagct cgtcgaagat tttctggatg atggtctcgc tggttttctc ctccgcctcg
    14161 gcctcgtagg taatggccac gcgatggcgc agcacgtcca tgccgatgct cttgacatcc
    14221 tgcggcgtga cgtagccccg gcctttgagg aaggcatagg ccttcgcggc cagcgtgagg
    14281 tagatggtgg cgcgcggcga ggcgccgagc tggatgaact cccggacctg gattttgtgc
    14341 gcctccgggt cgcgggtggc gcagaccagg tccacgatgt aatccttgac cttgtcatcc
    14401 atgtagatgt cgttgaggac ctggcgggca tggagaatct gccggggttc gaccacccgg
    14461 ccggtgaccg gggtgccgga ggtgcgggcc atgagctcga ggatttggcg ctcttcggcg
    14521 cgggaggggt agccgatctt gagcttgagc atgaagcggt caacctgcgc ctcgggcagc
    14581 gggtaggtgc cctcctgctc gatcgggttc tgcgtggcca gcaccagaaa gggttcctcg
    14641 agcttgaagg tgtggtcgcc gatggtgacc tgcttctcct gcatcgcttc gagcagcgcg
    14701 ctctgcactt tggcgggcgc gcggttgatt tcgtcggcca gcacgaggtt ggcgaagatc
    14761 ggccccttgc gggtcgtgaa cgcgcccgac tgcgggttgt aaatctgcgt gccgacgacg
    14821 tcggccggca gcatgtcggg cgtgaattgc agccgcgcga acttcacatt caggcagttg
    14881 gccagcgact tgaccgccag cgttttggcg agccccggca ccccttccag cagcacgtgg
    14941 ccattggcca gcagcccgat gaccagccgc tcgaccagat acgtctgccc cacgatcacc
    15001 ttgcccaact cgttaaacag cgggcgcaca aaggctcccg cttcctgcac cgccgcgtta
    15061 atggcgttga ttccagtatt cattggtttt gcaatcttac tgtgcgatgg acaattaaac
    15121 cagaaattgc gttcaaccga aaggccggaa aaccccgctt caccggcctg ttttcgccgg
    15181 gaggcggccg cccggggccg gcgggctggg gcaaaggacg tcggaatcct cccgtcgcgc
    15241 cgttcggcaa cgcggatttg tcaaacccgg cgatcgggta taaaatggct cgtgaaattg
    15301 cctttttcgg tgcctgtgcg gtttcgttcg gctctgccgg cgttcctggg attgcttctg
    15361 ctccgcgcct ccgggcccgc cgtgcaagcc gcgccggcca gcgcggagca ggcggcggcg
    15421 gcggtcgccg gctggctgga cttgtccgca acgcccttcg gcgaagccct gggccgatcc
    15481 gtgcggcagg tggacacggc cagcgacaaa gccggcaacc cgctttacca catcgtgagc
    15541 ctggcgcccg cgggctttgt ccttgtggcc gccgatgacc tggtcgagcc catcattggc
    15601 tttgccgccg ccggcaagtt tgttccgtcg gaggataatc ccctcggcgc gctggccagc
    15661 cgggatttgg ccggccgcat ggcccgcgcg cgcaaggccg gccccgcggt tccgggttcc
    15721 gccgccgccc aggcgcaagc caagtggcag cagctttgcg cccgggcgga cgacgccgcc
    15781 gccacgccca aggggcttat cgttgtgtcc gacctccggg tggcgccgct cctccagagt
    15841 acctgggacc aggaaacggc cgccggagtc gggctggccg cctgctataa ttattacacc
    15901 ccgccctacg gccccggccg caccaccaac tatccgtcgg gctgcgtcgc gacggcgatg
    15961 gcgcaattcc tgcgctacta ccagtatccc gacaccggcg tcgggacggc gagctttacc
    16021 atctccgtcc ggggtgttcc ccaggcctac tcgttgcgcg gcggcaacgg cagcggcggg
    16081 ccgtacctgt ggagcgatat gcccctggcg ccgcccgcca cccccaccac cgcccaatgc
    16141 caggcgatcg gcgcattagt cgcggatgcc ggcgccaccg tgaacaccga ttatgccgcc
    16201 aatggctcgg cggcgtcggt gctggacgtc aagggcgcgt tgctgggcac gttcaagttc
    16261 agccatgccg cgcaggggtg gaaaaacaat aagaatatcg gcgagagcct gacgcgcatg
    16321 atcaacccca acctggatgc ccgcgggccc gtcctgctgg gcatagacgg gcccaacggc
    16381 gggcatgcgg tggtggtgga cggctacggc taccatctgg ccacgctcta ccaccacctg
    16441 aacctgggct ggtcgggtgg cgccaatgcc tggtatgcgc tgcccttcat tgacgcggcg
    16501 gtttacacct tcaccacgat aaccaaatgt atttacaacg cctacacgaa cggcaccggc
    16561 gagatcatca gcggccgggt gctggaccag cgcgcccggc cggtcgccaa tgcgacggtt
    16621 tcggccgttc gcaccggcgg cggcacttat acggccacga ccgataccaa cggcatttat
    16681 gccctggccc ggattcccgc ctcctccacc tacacgctca ccgtcagcaa ggccgatttc
    16741 agcagcgccg tcagcaattt cacgaccggc gtctccgtga atttgacggc cacctgcggg
    16801 aattactggg gcgccaactt cacgctgaac cagctcccga ccgtgctgga tcgcctggaa
    16861 tggagcgcca tcggcgccac ccagagcgtc ggcgcgccgt tgagcgccac cctgaccgcc
    16921 ctgaacgccg acaacggcat cgtgactggt ttcgccggcc cggtggcgct cagcgcctgc
    16981 tccccgggcg ctctcgcgac caacaccctg ctcggaaacc tgggaagccc gcaatcccaa
    17041 accgacccca actacgcgtg gacccagggc tatgagttca caccggccac gaatttgctg
    17101 gccttcgccg ttcgctccta cagcggcacc aaaatcacgc tatggactgg ggcgggggcg
    17161 ttcatcgctt cccggagcgt gaccaacccg cccggcacct ggactgagac ggcgctggac
    17221 gcccccgttt tgctctcggc cggcacgccg tatcggttgg ccgccctttt cccggccggg
    17281 gtcccgcgat actacacggt ttacaccggg acgtggccca cgacgtttgc ccatggcacc
    17341 atcggccagg actgctatta catttacgaa gaaggcttcc ccaaccaggt cctgggctcg
    17401 ggatacggcc tctttctcga tctgcgctat accgttccat acacgaatga catcgccatc
    17461 accccccccg tgtcgggcag cttcgtcaat ggcgtctgga atggaaacat cacggtggaa
    17521 accgccgccg ccggggtggt gctcaaggcg gacgacggcg ccggccacat cggcttgagc
    17581 aaccccttca atgttatcgc gcccatccag ctccggtttc cccggatcac ggcgggaggc
    17641 cagttccaat gcaccgtctc cagcgccccg agcctgaagc tggaaatcct gggctcgacc
    17701 aacctcttaa actgggcgcc cgtggccact ctcaccaaca ccagcggcgc cttcccgttc
    17761 actgacccgg ccgcccttcg cgcggcccgc ttctaccgcg cccgccagtt gaattagccc
    17821 gcccggcaag ccgcgccgga tcacagccgc ggggcggtct ccggggtttc gggcgcccgg
    17881 gcgcgccgcc gccggttcgc ccccgggatc cgggccgccc ccggcccccg gtggcagaaa
    17941 aggcttgcgc ccggcagatg cctgccgtca ctctgaccgc agaccaagat gaaaacgaac
    18001 cggctctcca ctggcgttgg cacactgttg gttttactcg gaatggctgc cgggggcgcg
    18061 gcggctgcgt tcggccagga cgccgtatat gccgcgcggg ttgcctccca aagcgacacc
    18121 aattgggatt ttcccttcta ctggattaat gtcggcgact atacgcccta taacccttcc
    18181 accgaacaga gctcggcgac ccagcccgtc agcacccctt cccgcatagg ctcctatgtg
    18241 cataccgccc gcctgatgtc cgtgggcggc gggtttggac tggtccatat cgcgggcacc
    18301 aacgccaatg ccatttatga agtgcagatt acccagccgg gcaagggggt ggcgccggac
    18361 atcatcatga atgtaggctc gaccaacggc gacctgggcg gggtgttcgg cgcgacggcc
    18421 ggcggcggct ggaccaacac cacggctttc cagcggaatt attccagcaa tcaatgggcc
    18481 ttcacctgct acctcaccaa ccggccccgc gtcacccagc cgcacataga tttcaaatat
    18541 gtcagcggcg gcacgggttc ggaccgcacc tatgccgatt gcgtgcgatt ccatcagatc
    18601 ggcatccgca ccaacgtccc ttccccggtg cggatcacgc gcctggccgg cgcttccctg
    18661 gactacacgg gaggcgcggg cgcgcgcttt gtgctgctcc agtctcccag cctcccgagc
    18721 gcgcccgcca actggcaacg cctcgccacc aatacggcca cgcccggctc gttctcgatt
    18781 cccccggccg gcgcggcggc ggcggcgttt tattgcatcc aggccgagtg aaggtccggc
    18841 ttccccggcg agccgccgct cccgttctcc cacgcatgaa cctccatcca accgcgccaa
    18901 ccgccccgcg cccgcatcgc cgatgcccgg gcggcccgcg cgggcgccgc gccgaagccg
    18961 ccgccccggc gcgcttccat tcctgagcgt atgcgccgtt cgttgtcatt ctcgcgcggg
    19021 cggacgtccc cggcgccgtg ggggcggcgg ttccggtggc tggcggtggc gctgctgctt
    19081 tacacgctgg cggggttctt tctgctgccg gcgctccttc gctggcagct ggtcaagcgc
    19141 ctgcctggcc tgacgcatcg gcaggccgcc gtgcaaaaag tgaagttcaa cccgtatgcc
    19201 ctcacccttt ccatccaggg cctggcgctg acggagacga atgggacgcc cttcgccggc
    19261 tgggacgagc tgttcgtcaa cttccagctc tcctcgctct ttcaccgggc ctggaccttc
    19321 cgggaaatcc ggctggagcg cccgaccgcc acgatcctcc gcgccgcctc cgggcaatgc
    19381 aacttcgcca atctcggccg ccccgccccg gcgtccgcgc ccgcggcgga cgaaatcccg
    19441 aagccgccgc ccgcggtgcg gatcgggcat ttgatcgtca cgaatggcgt ggtcactttt
    19501 gccgaccaga ccccggcccg gccgatgcgc ctggagattc acccgctgaa cctggacctg
    19561 cggaacctgg ccacccgccg cgatgagaaa gggcaatacg cgctgacgct gcgcagcgcg
    19621 gacggcgggc attgggattg ggcggggacg ctcgcgctca acccgccgcg gtccgcgggc
    19681 acgttcacgg tgcgcggcgc gcgtttggcg gtcggcgcgc cctacctggc ggaactgacg
    19741 cgggcggaga tcgccgacgg ccggctggac gcctccgggg agtaccgcct ggcggcggag
    19801 ccggcgctgc aactggaaat cacccacgcc acggtgaaaa tcgagcacct gcagctcacc
    19861 gaccccggcg cgggcgaaac gctcctggcg ctgaaccagt tcgacgccgg ggacgcgtcc
    19921 ttccaccttg ccggccggca agtccgcctg ccgcgggtgg tggcccgggg cggcgcggcg
    19981 ctgctgcggc gcgcggacga cggacaatgg aattgggccc ggcagcttgt ggcgcgcccc
    20041 ccggcgcccc cggcgtccgc cgcgcccgcc gccgcgccgg cggaaacccg tgcgccgtgg
    20101 gaagtggccg tggcggagtt ccgcctggaa gacttcgcgg tcgtggcgga ggaccacgtc
    20161 cccgcgccgc ccgcgcggct ggggctggat gggttgcgca tccgggcaac cggtttttcc
    20221 acccggccca acgcgccgct ggcggcgacg gtggagggca aatggcgcgg cggcggcacg
    20281 gtgcgcatcg aggcggacgg cacggttcag cccccggccg ggacggcaaa gatcgccctg
    20341 gccggcctgg cgctgccccc gctgcaaccc tacctcgcgc ggcaactgcg gctggcgctg
    20401 cagtcgggcg agctcagcct caacggccag gcgcgctacg cgcccggcgc cgctcccgcc
    20461 ccgcgggcgc gatttgccgg cgacctggcg ctggcgaagt tcgccgtcgc ggagacgccg
    20521 ggcggccgcg aactggcgaa gtgggacgag ctggccctgc gcggaatcga gctggcgtgg
    20581 cataccaatg ccctgaacgt ggacgaagcc aggttccagg ggctgaaagc cagcctggtg
    20641 gtcaactcca atggccagct taacgtgacg gctttggccc cggcgccgcc cgccgccgcc
    20701 cccgcccgcg cccccgcaac gccgcccgcc gcggcggcgg ccccggcgcc cgcggcgttc
    20761 cccctccgga tcgggaccct ggccttcgcg cagtgctccc ttcaggccgc cgacgaatcg
    20821 ctcgcgccgc ccgtccatgc ccgcctcgag gagttcagcg gcacgatccg caacctggcc
    20881 ctgcccgcca ccggcccggc cggcgtggac cttcgcggca aaatcagcgc cctggcgccc
    20941 ttcgagatca ccggcgacat cacccccgag gcgaaaaatc tccaggtgga tttgaagctg
    21001 ggtctcaaaa acagcgacct ggcgccgctg gccccttaca gcgagaaata tgcgggccac
    21061 ccgctccacc ggggcaagct cagttgcgac gccgcctata aggtggccaa ccgccaactg
    21121 gacgcccgcc atacgatcgt gctggacggc ctggccctcg gcgcgcgcaa ccccgccgcc
    21181 aacgcgccga acctgcccct caagctcgcc gtggccctgc tcaaggaccg caacgggcgg
    21241 atcaccctcg atctgccggt cagcggcaac ctggacgacc cgaagttcaa gctcggcgcg
    21301 atcctgggaa aaacgctctc gaacctcctg ttgaaagtgg ccgcgtcgcc cttcgcgctg
    21361 ctgggcgcga tcaccggcgg cggcggcgag gatttgcaat acgtggactt ccccgccggc
    21421 gccgccacgc tggccggccc gcaaaccaac aagctcgccc ggctggcccg ggccctgcac
    21481 gagcgccccg ccctgggcct ggaaatcagc gcctccgcca acgccgtcac cgaccgcgag
    21541 gcgctgaccc ggcaaaaact gctggcccgc ctcaagcttg cgcgcgcgga ggaactggcc
    21601 gcggcgggcc aggcactgcc gcccccggat gagcttcaac tcgagcccgc ggactacgac
    21661 cgcctgttgc gcaaagtctg cgaagccgcc tccccggcgg cgccggaaaa cctccccgtg
    21721 ctggaagact gggccgccgc cgccgccgcc ccgcccgccg gcccggacgc gggcgccccc
    21781 gccgcccccc gctccgcccc ggctaccgag aaaggcgcct tgtggctcct gcgccgctta
    21841 ttcagcccgt cccccccgcc gcccgcgccc gccgccgccc cggagacggc ggacacgccc
    21901 gccgccgcgc cgcccgccgc ggccggcctg ggccgcgccg aaatggagca gcggctgatg
    21961 cgcgcgctgg ccgccaccga cgacgacctg cgcgaattga tacgccagcg ggcccaggcc
    22021 gtgcggcaat acttgcggga caccggcaaa gtcccggccg accggctctt tctcgcccct
    22081 ccgcaacccg tgccgcccgg caccaacggc ctggcgcggg ccacgttctc gctgggctga
    22141 tcctcccgcc gccgcgcggg cttcccggcc gcccgcgggc gccccgggcg ggagcggttg
    22201 gcggatcgcg acttgcacct tcccgccccc tctgcgactg taggccgtgg aatgcgcctg
    22261 accgaccggc aatcaaatga acgacgacat ccttccccgg ctgcctgagc ccaagcccaa
    22321 tgttgatgag ttccggcgcg tcttgcaggg cggcgactgc gggcgggttc cgctggtcga
    22381 gctggcgatt gccgaggaag tcctggccgc gctgcacggg cgccccttga ttccgctctc
    22441 ccgcgcggag ggctggcaga cgtttcgcgc gggcgtccgg cagcgggtcc ggctgcatcg
    22501 cgccctgggc tacgactact accgggtccg ggcggaaatc ccgttccaga tggaaaccct
    22561 gcccgcgcgc aacaccgccg ccgccgccgc cgccgaccgg caatgggtca atgaacagga
    22621 ggggctcatc aagaccgccg ccgacctgga aacctatccc tggcccacac gggcggccat
    22681 tgatttcgcg gcggcggaag cggcggcgga cgggctgccc gagggcatgg ggctgatcgg
    22741 cttctccggc ggcgtgctgg aatggtcaag caacctgctg ggactggaga gcatgatgct
    22801 gctgctttac gacgagcccg gcctcgtgca ggccgtcgtg gatcgggtcg ggcagatcat
    22861 ttacgaggcc tttcacgtct tttgccagat ggacggcgtg ttcgcgatct ggctggggga
    22921 cgacatgggc ttcaagaccg ccaccctgct ccagccgcgg cacctgcgcc aatacatcct
    22981 gccctggcac cagcgctacg ccgagctggc ccaccgcacc ggccgcctgt tcctgctcca
    23041 cagttgcggc catgtcgagg caatcatgcc cgacttgatc gagacggtgc gcattgacgc
    23101 caagcacagc tttgaagacg tgatcgtgcc ggtcgaggag ttcaagcggc gttgggggac
    23161 gcgcgtggcg gtgctgggcg gcgtggatgt ggacctgctc agccgcggcc ccgaagccgc
    23221 catccggcgc cgcaccgggc agattctcgc ggcctgcgcc ccgggcgggg gctatgcctg
    23281 cggctccggc aactccatca ccaactacat gcccgccgcc aattacgtcg cgatgctgga
    23341 aacgctgcat cgctttaacg ggcgcctgta agcgccgccg ccgcgcgccc cgcgcctccc
    23401 gccgccgccg cgccgccgcg gccggcgcgc cgggcttact cgatccggaa cagcccccgg
    23461 gccatgacgt aatgctcctg gatgttcatc ttttccatcc aggcgcgcac ctcgagcgtc
    23521 ttccaggggc ccgagccttt gccctggccc gaatcgttgt cccacagcca gagcggcgtc
    23581 ggccacaagc agcgggccgg ctggaccgcc tggtaaaacg cctcttccac tccgtcctgc
    23641 ccgtggtggg ccatctggac gtaatccgac ggcaggcgcg cggcccgggg cgagctcaat
    23701 aatttgcgcc ctccctccac gccgagatcg gcggtgaaga gaaccgactt ggccgcatcc
    23761 gaaacccgca gcaccaggct cgaattattg atggcgttga ccgtgatctc gggattatgc
    23821 acgcccagca cctcgaagcg cacgccgtca atcgtcatct cctgcccgag cgccaggtcc
    23881 cggatcacct ggttcgcctt gcgcaaggct tgctgaaact gctcgtaggg ccgggtatcc
    23941 tccgcgccca ccccgtgggc gttgacccat tcctgcgtgg gcagcgaccc gtagatcgcc
    24001 cggatgcgcg gcgcggcgcc ctgctccagc aactggctca aggcgccaaa atggtcatcg
    24061 tgcgcatggg tgataaacca ggcctccacc tcgttgccca gggccgccaa aaatcccttc
    24121 aagtaagccg cgtctccggt cgtgccgcca tccagcacga tcactttccc gcccgccgtg
    24181 cgcaggacat aggacataat ctggctgtgc gtttgattgg gcagttgcca gagcacgaag
    24241 gttttgtccg ccgccgcgcc cgggccgccg ccgcagcggc atccccaccc ggcgccaacc
    24301 gcccaaagcc cgatcagtaa aaaccagaag gcacgaagat tacgcataac ttacttctgc
    24361 cagaaatccc gcggcgggtc aattaaccgg ggcgaggttc ggggaaaaaa agagcctccc
    24421 cgcttcgcct gtccccggca gcgctccgcc acccgctgcg tcatccagtt ggcaatgacc
    24481 gcgccaacgc gccgcacatc gcgcgcccgg gatacgagct tcgccgcgga aaaggcaacg
    24541 tcccagggca ggggaccctc atgctctcgc cggcatattc agaatccctg cccgaagtcc
    24601 aactgtgcca atttggcaca ccccatttta agcagtgaat aactcaacgc cccctgcgta
    24661 atccccataa aacagcgatc gccttaatcc gaaaatacac cgccgaccag ggtaatattt
    24721 cctccatgac cgcgccaacg aactgtccaa ttattggaca cttctcccgc ctgctgccag
    24781 tcccaccccg cgctccttgc catcgctacc cgccccgatt ttgcgcaatc cggctcactc
    24841 caggctctaa aatagccaat aataggccgt gtacaacggg tgtacaaggg atgctcaccg
    24901 ggtgaacata ggattttagg ttgggcatcc ggtgagcatc ccttgtacac cccttgtaca
    24961 cggctataag agagcgggaa atgatgggaa cgacaggctg gagattggct gcgggatgca
    25021 gattgggcgg agggggattg gggggcgttg ccgtgttttt aaggggggcg agctggccct
    25081 ggtttgagga gcggccgatt caggacggcg gggttaaggt gtgccaaatt ggcacagttc
    25141 ggcacccgaa gcaggggagc gatcggggca aacggaacct gccggatggc ggttggggca
    25201 aggcggtcga agccgggtgt tttttcgcgg aggctaaagt gcgccagtcc ggggagactg
    25261 cgtcgagtga gggagcgagg aggtgagttg gcccctttgg cggggccaat ctcgctattt
    25321 cattcgccgc gcgaaatgtt aaccggccgg gaggcggagc ctggaacggc tttcgctact
    25381 gagaaccggc aagtgggcag ggtgatcgtg cgccgggttc gcaggattga aatgctcagg
    25441 gcggcgtctt gcgggcatgc ttgcgccacc agcggcggta atcggtgtag agttgggtgg
    25501 agtccgggtg gccggcaaag gctttgctgc cgggtttatt catcaggccc tggtattgat
    25561 agatcaggat ggtgtccacg aacggggaca cggcggccaa ctgttccttc acccgcgaga
    25621 aaggcgcggg aatgaggggg ctgctctgct ggttgggcgg gccttcccag gcgaaggttt
    25681 ccacgtccgc ccacagcgcg cgctggggca cgcggtggtg cgcctggcgc aatttctcga
    25741 aggcggccgc gctttgcgac gggttcatgc gccggcagcc gacttcgtcc tggtaggcga
    25801 tgatgtccac ctcgagttgt tccagttggc gcacgaaccg atcgtcgttg accgcccggt
    25861 gggtgccgta gggggcgata agcgtccggg cccggggggt gaggcggcgc gcttcgcggc
    25921 tgcaggcgtt gacgtaggcg atgaattcgt cggtgaaata ggggccgagc gcggcttcgt
    25981 tgggccaata ccagccgtga aagctgcggt ggtggctgta ttgccgcgcg atctcgctca
    26041 tcatctggaa ccgcgcccgc acccgcgccg ggtcgtgcag gcaccggtgg tcccaggggc
    26101 cgaaccaatc gctgctgagg aagaatttga cgccgtattt atccgccgcc gccaggagcg
    26161 cctcgagggg gtccgcgcag gccagttcga gtttgggcag caggggggtg gtgtagaatg
    26221 cctggccgcc tttggcgatg gccagcagga cgaggtattc catgccgagt tcggcgactt
    26281 cccggacggc ctggcgccat tgttgggcgg tgtaattccg gcaggtgtca ttccagtaaa
    26341 ggtggcggcg gtcatcccac cagatgctga tccagctgcc gcggatgggc tggatgcggg
    26401 cgacggggcg ttcgccgggc gggccgctcc gcgcgaggtt gagcgaggcg gcggccacgc
    26461 cggccccgag caggcggagg aacgccgcgc gcgagagccg gggcgggggg gggccggcgg
    26521 gcggcttatt tgtcgcggat gtattcgtag atgttgaggt agtgctggcc ggggtggttc
    26581 caggagaaat catagcgcat gccgttgagc gtgagttcgc ggaaatactg aggataggca
    26641 taccacaggc caatggcgcg gtgcagcgcg gattcgatgc ccgtgttatt gtagtcgttg
    26701 aagacaaagc cgttgcgttc gtggtaaggg cggtcggcgt agttggcgtc gaacacggtg
    26761 tcggccaggc cgccggtggc ccggacgatg gggacggtgc cgtatttgag cgcgatcatt
    26821 tgcgtcaggc cgcagggttc gaacaggctg gggacgatga tcatttcggc gccggcatag
    26881 accaagtggg agagttcttc gttgaagctc agttcgagat ggcagtcggg gttgtcgttg
    26941 tagagttgtt tgaggcgcca gaagtggtgg ccgatgccac cgttggggct ggtgcccagc
    27001 agaacaaact gggtgccgtg ctggaggcag tagtgcaggg cgtgggtgat gagggggacg
    27061 cctttctggt ggtccagccg gccgatatag cagacgatgg gcttgtaggc gtcctggagc
    27121 cagaaccgct ggcgcagcgc gtgtttgttt tcatatttgg cgtcgaggtg gttgaggctg
    27181 taggggcgcg ggaggtggcg gtcgatttcc gggttccaga cctcgtagtc cacgccgttg
    27241 aggatgccgc ccagtttggc ccggtggacg tacagggtat ggcccaggcc gaagttatgg
    27301 gaggtgttga ggatttccca ggcgtagcgg ggcgagacgg tggtgacgaa gttggcatag
    27361 acgatgccgc ctttcatcag gttgagggcg gcgtgattga agttgtcctg caggcggtcg
    27421 gggtgaaaga agtgggcgtg gcggttgagg ccggtggcgc ggaggatatg ctcgccagtg
    27481 aggccctggt gctggaggtt gtggagggtg tagcacaccc gggggtggcg catgcccgcg
    27541 tgctggtata tttcataaag caggaccggc accagcccgg tctgccaatc gtggcagtga
    27601 atgacgtccg ggtgcttccc gctctggagc atgaactcca gcgccgcctt gcagaaaaag
    27661 gcgaaacgct ggtcgtcgtc gtgctgcccg tagaagacgc ggcggtgaaa gaaatgatgc
    27721 tcggagtgcg gttcgaggaa gaagcatttg cggccatgca cgaagccgaa atagacgctg
    27781 caatggatgg cgccgttgtg ccacggcacc cagaggtcct ggaaggtgag ctgcaggccg
    27841 tagatctggt cgtagcgcag gcagtcgtat ttgggcagga tgatttcgac cgagttgccg
    27901 cggatttcca gttcccggct caggccgaag acgacgtcgc ccagcccgcc caccttggcc
    27961 accggggcgc actcgctggc aatttgcacg aggtacatat tcgggttagg cgttgcgcag
    28021 ttggtgcatg gccgcatcca gcagggcgta ggcctgctcc agctcgtcaa acgagcggga
    28081 cacccagcgg ctgccccagt agaagttgca actggtttcg gcggccagca ggtgatcgaa
    28141 ggcgcgcacg atcaggcgcc gtatctcctc cgggtgggtc agcgccgcca gttgctggtc
    28201 gaaggcgcgc ttgacctgcc agtagtattc gctggcgcgg cgcacttcgt cccatccctt
    28261 tttctgcagc agcgagccgg tccactgcgt gaaatcgccg ccccactggt gctcggtatt
    28321 ccaggcgccg ggatgcacgt ccacttcctc ggtgggcggg tattgcgcca ggtattggct
    28381 gatgtggatg ggggtgaagc ccagcgtgcc cgcccggaac cggtccagga gggggcggta
    28441 gaagaagccc cagaagccgc tctcgatttt cgtcgtgcgg aaccagccgc cgttctcgcc
    28501 gtccgtccag gtcgtcacca gcgccgggaa atcgcaaggc cgggtgcgct cgtggatttc
    28561 ctgctggaac cagccggggt cggtgcccga gagttgggcg ttggagagtt cgcggtcgcg
    28621 cggcacgaca atgatctccg cgccctcgta gcgcgcccgg tagggccggt agcggagttc
    28681 ctcccagcgc atggggcgcc ggggtttgag gtaaatcgaa tccaccagca cgtactggta
    28741 accgtgccgc gccagcatgg ggatcatttc catgcagaac cccatctccg gcggccagaa
    28801 ccccgggaac cggtcccgcc cgagcaggtg ccgccccagc ccctgccacc attcggtctg
    28861 ggcgtcccag tccgcttggg gggtcagcgg gtacaccggg tgatacagcc cgcttcccag
    28921 gtactcgatg ttcgcgcgcc ggtaccggtt caggaaatcc tcgatgtccg ccacctccgc
    28981 gaaggtctgc cgcacgccgg ggtcttcgag ttgcttgagc agcgtgccgg agaagctcag
    29041 gtgcagccgg gccacgtctt catagccctc gagcatgcgc gtggggcggt cgtagcacca
    29101 gagaatctgc cgcgcctccc agctttccgc ggaattatgc agcgccacga ggttccccgg
    29161 cggctggtgc atgtgcaacc ctaacgcgtg ataaatctcg gccatacaag agtctggttg
    29221 aatacggtcc gggcaagcct accgtgaatg gcggcggttt caagggcgta tgtgggggcg
    29281 gatttcactg gacaagcggc gcggggcgcg gcagggtgga ggcatcgtgg caatacagtc
    29341 gttaaactaa ggcatcgcgt ttgaattgaa ttatgacacc ggtttcccgt cgcaccttcc
    29401 tgggccagtc cgcgctcacc gccgggctcg cgcccctcgc cctcagcagc ctcacttcca
    29461 gcgtctttgt ccgcagcgcg cgcgccggtg agccaggccc gaatggcaag gtgcggctgg
    29521 ggatcatcgg ctgcggcggc atggggcagg gtgacctggc gtgtttcctc cagaatcccg
    29581 aggtggactg cgtcatggtc tgcgacgtgg atgatgcccg gatcgccaaa ggagtggaga
    29641 tttgccagca gaagcgcggc cggaagccgg acacggcgcg ggatttccgc cgggtgctgg
    29701 atcgcaagga cgtggacgcc gtgctcatcg ctacgcccga tcattggcac gccctgccca
    29761 cggttctggc ctgccaggcg gggaaggatg tgtacgtcga gaagccgctg gccaccacca
    29821 ttgacgaggg gcgcgcgatg ctcgaggcgg cgcggcggca tcagcgcgtg gtgcagatgg
    29881 gctcccagtg gcgcagttgc aaccatatcg tcgaggcggg cgaattcatc cgctccggca
    29941 agctcggcaa ggtcagcatc gtgcgcggct gggcgtacct ggactggctg cccagtattg
    30001 gcaagccggc ggattgcccc ccgccgccgg gagttgacta tgacctgtgg ctggggcccg
    30061 cccccgaacg gccgttcaat cccaaccggt tccacttcaa cttccgctgg ttctgggact
    30121 acgcgggcgg cttgatgacc gactggggcg tgcatctcat caacatgatg ctcatgggca
    30181 tgaaccggga agcgccccgg agcgcctact cctgcggtgg caagttcgtt ctggatgaca
    30241 actcggagac cccggattcc caggtgacgg tctatgagtt tcccggcttt accctcatct
    30301 gggagcataa ggccggtctg gacaatggcc tgtataaccg ttcatggggc gtcgaatgga
    30361 gcggcaccga aggcaatatc atcctgaatg accaggggtg ggagatccgc acggaaaaga
    30421 agcgggccaa cctcgataac cagcgcaagc ccggcagccc cgacccgcgc ccggcccatg
    30481 tgcgcgactt cctggactgt atgaaatcgc gccggcagcc cgtgctcaac ctggacgtcg
    30541 gccatcacgt gtccaccgtg gcgcaccttg gcaacattgc ctaccgcgtc ggccgcaaga
    30601 ttgtttggga tgccgccgcc gagcgggtgg agggcgacgc ccaggccgac aagctggtgg
    30661 gggtgcgcta tcgcaagccg tggaagctgc cgtacgccaa gcgcgcatag cgggcgcggg
    30721 cgcgggcggt tgagggacta tccacgccgg ggtgtcccgg ccgcgccgcc gccggggcgg
    30781 ggagccgccg tgtggcggaa tctcagagca gcttgctcag cagatcgtcg ttggtcttgt
    30841 gctttcggag cgcggccacc agggtttcca tcgcttccac cgggttgagg tccaccatgg
    30901 tgcggcggag cttgcgcacg gcttcgatct ggtgcttggg gaagagcttt tcctccttac
    30961 gcgtgccgct cttggggatg tcaatcgccg ggaagaggcg ccgatccgac agcttgcggt
    31021 ctaaaatcaa ttccatgttg ccggtgccct tgaactcctg gaagatcagc tcatccatgc
    31081 gcgagccggt atccaccagc gcggtggcca ggatcgtcag cgagccgccc tcttcgatct
    31141 tgcgggccga ggcgaacatt ttgcggggga tttccagcgc gcgcgcgtcc acgccgcctg
    31201 tcatggtgcg gccgctgccg ccgtgcgcgc tgttgtaggc gcgcgccacg cgggtgatgg
    31261 agtcgagcag cacgaagacg tccttgcccg attccaccat gcgccggcac cgctcgatca
    31321 tgaagcggga aaggcgcacg tgcgtctcca ggtcctggtc gttggacgag gccacgactt
    31381 cggctttgac ggagcgctga aagtcggtga cctcttccgg gcgttcgtca atgagcagca
    31441 ccagcacgtg gacttccggg tggttggccg tgatggcgtt ggcgatttgc ttgaggatgg
    31501 tggtcttgcc ggtgcggggc ggggcgacaa tcagcccgcg ggtgcccttg cccagcgggg
    31561 tcaccatgtc aatcacgcgg gtctcgatga ggtcgggcgt ggtttcgagg cgaaacttct
    31621 cgatgggatc aatcgtggtg aggttctcga accgcaccgt cttggtgtaa tccgagaagg
    31681 ggactccgtt aaccttgtcc accgacttga gctgcgggct gttgccgcgg tgcggcggct
    31741 gagtggggcc ggccacgagg cagccctcgc gcagaaagct ccgcttgatg gtgtccggcg
    31801 tgacaaagat gtccgacggt ttgggatgaa aatgattttc ggcggtgcgg agaaagccga
    31861 agcccttctc ggagatttcc aggtagccgg cgccttcagt ggtggcggca gtatttgcgt
    31921 tgtcggtatt ggcagcagtg ctcatacaag tctgtgtgtc atcgaaggct ttccgaatga
    31981 atcccggaaa accacttatg ggcttggaat tcttgagaag ccgcagcgga gggtggtctt
    32041 cgccgaaccc gcccaccgcg gttaacaaac cttcgatgcc cctataacta ccgttaaccc
    32101 ccggggaccg caacaactat tttcagatat ctttacccgc ccagttgagc ccgggcttcg
    32161 tccgccaggg cggtgctcag gtagcgctcg ccggtggaat tggcgatggt gacaatcagc
    32221 ttgccccggt tttccggccg ccgggcgacc tggatcgcgg cccaaacatt ggcgcccgag
    32281 gaaatgcccg cgaggatgcc ctcttcccgg gccagccgcc gggccatggc aaaggcgtct
    32341 tcattgctca cctggatgca ctccacgatc tgcggattgc cgcgggcgtc tttgaggtgc
    32401 aggtttccag ggatgaagcc ggcgcccgct ccctggattt tatgcggtcc gggcttgagc
    32461 ggctggccgg cgagggtttg cgagatgacc ggggagtctt tgggctcgac ggcaatggcc
    32521 tgcaaggagg gcttgcgcgc cttgagcacc tcgcagcagc cggtcagggt gccgccggtg
    32581 cccaccgcgg cgacgacaat atccaccttg ccgtccgtgt cggcccagat ttcctcggcc
    32641 gtggttttgc ggtgaacctc ggggttggcc gggttctcga actgttgcgg catccaggcg
    32701 tgcggggttt cccgggccaa ttgcgccgct ttggcgatgg cgcccttcat gccctccgcg
    32761 ccgggggtga ggacgagttt ggcgcccagc atggccagca gcgtgcgccg ctcctgcgac
    32821 atcgtttccg gcatggtgag gatgagccgg tagcccttgg ccgcggcgac aaacgccagg
    32881 gcgatgccgg tattgccgct ggtgggctcg atgatcaccg tgtctttcgt gagcaccccg
    32941 cgccgctccg catcttcaat catcgcctgc ccgatgcggt ccttgacgct gcccagcggg
    33001 ttgaagaatt cgcacttgag caaaatcgtg gccggcgctt ccgcggtgac tttgttcaag
    33061 cgcaccagcg gcgtgcgccc gacggtttcc acaatattgt tatagatacg gctcataata
    33121 tttcaaaagc taagggcgcg agcccgggtt aacagtaatt cgcgcgctaa tgttcactat
    33181 aaaccagggt tgggcaagtg gaaagatgcc ggccgccaaa ggcccggggt gaaaaccaaa
    33241 agtccctgcc cggccggggg gccgaacagg gactcaatgg gggatgggag ttcgccacgc
    33301 tctctttccc ggtgctggca ttaggcgccg aaccggctgc cagcttcccg acgctattct
    33361 ccacggcaag cggagttacc ggcctcatag cagggccgtg cagcgccttg cggcggctga
    33421 attattgagt gaggcttttc agctcgcttg ggctggtggt tgcgccaccg gcttgcgaac
    33481 caggtcaaga cacgtctgcg tcttattatc cgcgtcttca ccacttagcg ccgggacggc
    33541 cgctgctttt ggcagcgcgt tggcccggtg ccacgggtct cgcctcttaa ctctcaggtt
    33601 gcctttagtc cacaggccag cggtgtcgtt tttaacatcg ccgactcttt ggaaggtttt
    33661 cacgagccgt gccgaggccg cgtgtccttc ccggttcgga cgctcacttt gtctcgcctt
    33721 tcagcgtctt tccttgggcc gacctccttc cggagaccgc cagagccgcc cgcccgtgct
    33781 ggtgcacagt agcaaccgga taccctctgg gtaactgcga tgggcccagg ctcgaaacct
    33841 gcccgggcgc cttgactcga ttcggagcgg tttctcatcg ctcctgatcg ctcagtccct
    33901 ttttcgcgtt tactgacagc gcgcgaacgc tgccagagga actggtgaat cctgcctctg
    33961 ccaccggcct ttacgatccg gcttgggagg ggttgtgcca accagcctgg cccaaccttt
    34021 ccccagtcac ctgggattat ctctgaaact gcggttcaat gcgttgactg ttcccgcacg
    34081 tctagttctt tcaatgaact gctcatagct tacccgagat tccatttccg acaacaaatc
    34141 cttatgcaca gcttattcac cgattcggcc ggatgccggg ttggtgtggc aggcaaatca
    34201 cccaatgggg ccaagggttt cctgcctcgg gctcgcgagt tattcacaat gccaaacttt
    34261 catccccggc ggcggtgaac aactttttct ccctgcggtt gacagtttcg acggaaaagc
    34321 tggccggaac gattgagcgg agcaggtctt gcgaacgccg ccatgccggg ggcgcgactg
    34381 gcgaaatggc aacgacagca ccgggccggc gcgcggaggc ggccggtccg gcaacagagc
    34441 gggagtggcc ctcggacatc gcccgggttg agcccggaga atgcgcccgc ccaggcccgg
    34501 caacggcagg cgcggagaaa ggagcgcgcc gcgggttggc cgcggggcgc gggagggggt
    34561 tcaggcagcc ggcgcggctg ccggggcgga tttggccttc cctttagcct tggcggcagt
    34621 ttttttgcgc ttcaaataag cgtttcgccg ttttcgcttc tcgaccttat tgtattgctt
    34681 gcccataaca cgctttactt tctgaatcta tttcgattgt ttgacccact atgaagactc
    34741 gttgggaacg attcaatatc tatttgctgc tggcggcggc ggtcgcggcg gtggtgctct
    34801 gcggatgcca aagcgccgct ccatccaaac ccggcaagct gctcgccgtt ctgcgcctgc
    34861 acctcgagac cacccccgac ggaaccaaca cgctgaacca catcccggtt tatcgcctga
    34921 atcccgtcca gatctgcgcg caaaagcacg cctttctcga cgaaggccat gtcgccgagg
    34981 ccaaggtaat cgaggtcgtg ggcggctttg cgctttggat caaattcaac tccgccggca
    35041 ccgcgctgtt cgaggaatac accacggcca accgcggccg gcggatcgcc gtcttcagcc
    35101 agtttggcga gcggatcaaa gactcgcgct ggctggccgc tcccatcatc tcccggcgcg
    35161 tgaccgatgg cattttcacc ttcaccccgg acgcgacccg ttcggaagcg gaggaaatcg
    35221 cgcttggcct gaacaacgtg gcccggaagg tccagaaata gcgcccgccc gcagcggcgt
    35281 tttcgcccgg ggaaccgccg cccgtcgcgc gcgcggtttt tgctcgcaaa cgtccggcct
    35341 tgcggctagg ctgtaaacgt aaaagcatgc gtgagccggg tatgaaaccc caggttgatt
    35401 tcgagtggcg cggcgggccg gcagcgaacg cccgccggag ggcggcgcgt ggcgcgcggg
    35461 gttccgcgtt cacgttgatc gagttgctgg tcgtcattgc catcatcgcc ctcctggcgg
    35521 cgctgttgct ccccgcgctg gcgcgggcca aagacaaggc ctggaccgtt ggctgcatga
    35581 ataacctcaa acagttggcg ctatgctggc acctgtatgc gaccgacaat gccgatctgc
    35641 tggcgccgaa caactcgatc atggtcttta gcggcccctc atggagcgtg ggcgcggcca
    35701 gcatttcctg gtgcccggat tccccccgca ccgacaccaa caccctggct ttgcaaagcg
    35761 gcgtgttgtt tccctacaac acctcggtgg gcatttatca ctgccccgcg gatcagtcga
    35821 aggtggagag cgcgggcctg ccgacggcgc agcggcgcaa ccgcagctac aacatgagcc
    35881 aatcggtgaa tgggtttccc gaatacctgg agcaactggg cattcccggc ctttcggaca
    35941 ttccctcctg gcggaaactc accgagattc ggcgccctgt gccgagccgg cttttcgtgt
    36001 tcattgacga gcacgaagat accctctacg acgcccagtt tgggaacccg gccggcgcgc
    36061 cctggtgggg gcccatgtgg ttcgatatgc cggccgatcg gcataatcgg ggcggttgcc
    36121 tgacttttgc cgacgggcat gtcgagcgat ggcgctggaa ggtggccaaa accggggcct
    36181 atcccggaaa ggtcccggac gtcggcgaaa tgcccgacta tcaccgcatc caggacgcca
    36241 tgaagtgctg gaaagacaag tgatcccggg gcgcggccgc cgggacggtt ccgccgcgcc
    36301 ggttcacggc gacttgccgc ttgcgctccg ggcgggagcc gataatcctc gggcgcacaa
    36361 ttatgaacta caacatttcc cggcaagccg cagcgctgtc cccctcgctg acactggcga
    36421 ttgacgccaa agccaaggcc atgaaggccg cgggggagga cgtggtgggg tttggcgccg
    36481 gcgaaccgga tttcgacacc cccgcgcaca tcaaggaagc ctgcgcgcgg gcgctggcgg
    36541 acgggttcac caagtacacc cccgccgccg gcctgccgga gctgcgggcc gccatcgccg
    36601 acaaacacca gcgcgagaat gggctggcct ttaaaccgtc gcagatcatc gtctcgtgcg
    36661 gcggcaaaca ttcctgcttc aacgtcattt tcgccacctg ccaggagggg gacgaagtga
    36721 tcatccccgc cccctactgg ctgagctacc cggagatggt gaaaatggcc ggcgcccggc
    36781 cggtcatcgt tcccacgacc gaccgcaccg agttcaagct cacggcggat gccttgcgcg
    36841 ccgccatcac cccgcgcacg cgcctgttca ttctcaactc gcccagcaac cccaccggct
    36901 cggtttatac gccggacgaa atcaaggccc tgggcgacgt ctgcctcgaa caccgcgtcc
    36961 tcatcatgag cgatgagatt tacgaacact tgctctacga tggcgcggtg gcccggagcg
    37021 tggccgggtg ctcccccgcc cacgccgcgc aaaccattat cgtgcatggc ttcgccaaag
    37081 cctggagcat gaccggctgg cggctgggct ggtgcgccgc gcccgagccg attgcccggg
    37141 cgattgacgc cgtgcaaagc cacagcacca gcaaccccac cagcttcgcc caaaaaggcg
    37201 ccgtcgccgc gttgacgggc ccgcaggacc atctccagac ctggctggcg gaattcgacc
    37261 ggcgccgcac ccgcgcctgg aatgccctca accgcattcc tggcctgagc tgcgtcaaag
    37321 ccaaaggcgc cttctacctc ttcccgaata tcgccggcac cggcctgaaa tccaccgact
    37381 tctgcgcccg gctgctcgaa gccgaaaaag tggccgccgt ccccggcatc gcgttcggcg
    37441 ccgacgatta catccgcttg agctacgcca ccagcatggc gaatatcgag aagggactgg
    37501 agcggatcga acggttctgc cgatcactgg cgcgctgagg cgcgaaaccg gcggttaacc
    37561 ggcgccgcgg ccaggcgcgc gcgccgcctg tccatccggc ggcgatccgc cgggagcagg
    37621 acgcgggaaa aaaggggacg ccttaacggc aggaaagcca gcgcccataa cggccttcac
    37681 gcatgtaggc gcgctcggtg ccatctccca acacctcgaa gagcttggcc gcccgcaacc
    37741 ggtagcggcg gtggcagttc aagcactcgt actcgatgag cggcggctca ttcggcgcgg
    37801 tttcagtgtc cttgaccacg agccagttgg cccccgcgtg gacgggccag ttggacggcc
    37861 gcggctgatg acaatggcat tggggaagca accgggtcgc aatttcccgc gtgggcggaa
    37921 gggaggggtc aggggcattc ataaatatgc aatctattgc cgttccgcta taacactgcg
    37981 cctctggccc aggatttcaa gcccaatttc tgtcccgaac tcgaaccgac ccgggcaacg
    38041 cgccgtgccg ggtttgccgt tcacacgctc aaatccccga tcacgcaagc cccgatcctt
    38101 ttttacgcat tcgcccgccg gatttccaca accccccgga cgaggcaatc tatggcctgg
    38161 ctgaacggca aatccgctgc gattcatcgt ccaacggaag ccgatttccc agccattgcg
    38221 ccgtcatagt ggttctacat cgttaacgta gccaggggga gccgggattt agtggagcag
    38281 ttcaaccgcc cgcccttgtt tcagcggatc ctgggcgggc ccagccgccc aaaccgcagc
    38341 cctggcggac tctttgttta ataaattcaa gggcacgcct gctgaatccc aggtctttcg
    38401 ccgataaaat caatagatca ggcataaaaa ggggtaaaat agaggcaaaa cccgaatcat
    38461 gccgacttcg gcgcaccctg aacgccccct gaatttaccc tgcctttagg ccgtgtacaa
    38521 ggggtgtaca agggatgctc atcgggagtc cattggattt tagctggtac acccggtggg
    38581 catcccttgt acaccctttg tacacggcgc atggctaggg gtaggcaatg atggtaacga
    38641 ctataaattc aataggttag agaagccgtg gggaaatcct ctttcctcac ataaaagctt
    38701 gaaaaggaat ggcctctgaa ccaaagcatt gctggggaag aagctcggca gaatgaaatg
    38761 aaccagtcgc ggttaaatga catcattctg gaggccggaa gctcctctct gcggcctcct
    38821 cgccgccgag gagaaacaga aactcaaagc gcatccccag cggcagtcgc cgtcataacc
    38881 agggagggca attcgggcgc aaataatgtt tggcgttttt ttactggcca actgaggctt
    38941 cttagggtag tctccccaat agttatgtat tcaactacgc gtgctataat tcgtttcact
    39001 ctcattcggc gcataattgc aggcttgggc tgtttgctgc tggcacacgc cctggcatgt
    39061 tcggcggccc tggtctttta tactgatgcg cagggcgagt tggtagg
//
LOCUS       DAOPQG010000023        38662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1413_length_38662_cov_13.659026, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000023 DAOPQG010000000
VERSION     DAOPQG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 38662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38662
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1413_length_38662_cov_13.659026"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            634..1674
                     /locus_tag="PKI07_04110"
     CDS             634..1674
                     /locus_tag="PKI07_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC50268.1"
                     /translation="MKATKLPGFIVALISALAAMSAAAQIMIETVPIGNVGNPNDATG
                     LGSVAYSYQIGKYEVTLNQYTAFLNAVGATDTYGLYNPLMGSFAQSLGIARSGTAGSY
                     TYSVIGSGERPVTWVSWFDAARFVNWLENGQPTGAQDASTTERGAYTLDGALSGVNFT
                     RNANATYGLPTENEWYKAAYYQPADQGGDSDDYWLYPTRSNLQPNSRNGSATDPNSAN
                     YFYNDGIPNGYNGGFAINNSTTYPTGNALTDVGAFSLASSYYGTFDQGGNVSEWNDTV
                     IEAFAYRAYRGGSWFYGVGNLRADSDLNGLAPDQERETLGFRIVVIPEPSAVGLMVLG
                     AALLLGRRKRAC"
     gene            2092..3465
                     /locus_tag="PKI07_04115"
     CDS             2092..3465
                     /locus_tag="PKI07_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC50269.1"
                     /translation="MTPTLLIVDDEKTTRDGLRAALEHRYDVYLAEDAKAAMELLEAE
                     HFDVLLTDFRLPTEDGMKLIMRAKSLSRPPICILMTAYGSEELAVEAMKHGADDYIAK
                     GRLQLDELEMRITNALRRQRLETENAALRQQLDTRFGLQNLIGESPGMKEVFEMVQQV
                     APTRATVLLLGESGTGKEMVAKAIHQLSPRAQHPLVTVHCAALSPTLLESELFGHEKG
                     AFTGAYERRLGRFEQAQSGTLFLDEIGEIDATIQIKLLRFLGERTFERVGSNKTLSAD
                     VRLIAATNKNLEAQVKAGAFREDLYFRLRVVELRLPPLRERLEDIPLLARAFQSEFSR
                     ENEKPLKGFTTEAFDALMRYPWPGNVRELRTAIEHAVILCRGEKITLRDLPLALREAV
                     SRTPDEKDIKRLLARQDLTSKEIERERIISALKATQGNRTQAARRLGMSRRNFHRKLH
                     LHRIEEI"
     gene            3468..5306
                     /locus_tag="PKI07_04120"
     CDS             3468..5306
                     /locus_tag="PKI07_04120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC50270.1"
                     /translation="MPFLQDLLHKLEIGGGMRYLRILPALMTIVLLGVIYNWRGYKNM
                     ATQEAMDAAQVARNLAEGKGYTTLFIRPFSIYLLKKRAQEKGEMPKLGEVADPAHLKG
                     MHPDLANPPGYPVVLAGLLKLLRPDYAVSTTQPFWSDSGRFWRYKPDFLITLFNQLLF
                     LAAIPVVFFLARHLFDSGVAWLSAVLLLGTELFWRFSVSGLSTLLLLLIFLGLVWSIV
                     LLEHEVRAPRWGRYGVFILAGLAGAMTGIGGLTRYAFGWLILPVLVFLILYGGQRRIV
                     LTLTTLAVFAAIMTPWMARNYDLSGAPFGTATYSVVETTMLSPEYRLQRSLEPDFSRL
                     YLMAFWLKLNNNLRQIVTSDLPRLGGSWVSAFFLVGLLIGFNNPSTQRMRYFLLGCVV
                     VLCLAQALGRTQLSEDVPEINSENLLVLVAPLVLIYGVSLFFLLLERIPLPFPQMRYL
                     VMALFSLAVCLPMLFVFLPPKTNPVAYPPYYPPAIQTIAGWMKEKELVMSDIPWAVAW
                     YGQRQCVWLTLKCTADSRDAGSGESFFAINDYYKPINALYLTPRTMDARFLSQWIRAG
                     DQSWGNFILESLLQKRVPGYFPLSESQSGWLPEQLVLTDWQRWRKP"
     gene            5362..5787
                     /locus_tag="PKI07_04125"
     CDS             5362..5787
                     /locus_tag="PKI07_04125"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HOC50271.1"
                     /translation="MSEIIYPRSPREVMCGWMHLPRFIDKTRLHLAGKLHPDYQPNFC
                     KGFDALWLETAGVEAQQFIEVVRARITDGEVCDWVRQNVLKPESVKAAHREQMLNYPR
                     KDDAQMQALLKLRKEQAGLAHREDIQNFVDFIDADEKRI"
     gene            5983..6936
                     /locus_tag="PKI07_04130"
     CDS             5983..6936
                     /locus_tag="PKI07_04130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HOC50272.1"
                     /translation="MILCLGTTPAAQRVMVFHRLTLDAVNRAVTTLDGAAGKSVNVAK
                     TLKALGEQPVAVGFLGGDRGAEVRALLAAQNIAADFVNVSARTRQCTTLLDQAAGTIT
                     ELVEEGQPVSTADYAALLDILRRRARGARAIVMSGSITPGGPADLYFHGTQIARANGA
                     LAIVDAQGAALAEALKAGPDLVKPNRLELAATLGRELPDEAAVIAGMQELCKRGALRV
                     VVTSGQGPTLAYDGQNGWKIQAPQVKTTNPIGSGDAFTAGLAWRLVRGENLGEACRWG
                     AAAGTANALTLMAGEVHRADVERLAAQVSVEPLPARSPGFF"
     gene            7402..8028
                     /locus_tag="PKI07_04135"
     CDS             7402..8028
                     /locus_tag="PKI07_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50273.1"
                     /translation="MPHVSELQRKPANHAGAGLIGGANRAQPLWIAPARAALRGGKRI
                     PASRGFTLTEVVISLAIIALVFGGILMAYVQAARKAEWSGYSLAAQAIGIHQIEQARS
                     AVWDYSIDKNELTNLNLNGWSYNKITRTGKGWTSRVLDIPRSGTNVVVATNFVTVKLL
                     SVTGLTNVHVHMVMVDTVWRFPMGNSTRLFTNQTASYFGPDNRDVSSL"
     gene            8025..8654
                     /locus_tag="PKI07_04140"
     CDS             8025..8654
                     /locus_tag="PKI07_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50274.1"
                     /translation="MKMPAIWRGTAEPPRRRRAGFTLSELLVSSAVFSLAIMGFVYCQ
                     MFGMRQDQLVNSKIGATELARLSFNELCNDIRAAKVWQIGNGSLGSFTPIPLGDNQKG
                     NALKLSMTINTNSYYLYYFDTNACKLYRGHSGSAALKCMAAYLTNTMYFQATDYRGEN
                     QTDLTHKGVINVVMQFHQYQYPITKIGPGQLYDYYRIELQATPHVPDGP"
     gene            8680..10473
                     /locus_tag="PKI07_04145"
     CDS             8680..10473
                     /locus_tag="PKI07_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50275.1"
                     /translation="MQTNATRARRNGYALLMVLTATGIASLVLAGTLNRSLTNAKMND
                     RSNEYSVASNAAEAAVEKVVARMGHDFQNYGPGAVAANINLYRNSIPDEDSFWTQFAF
                     SDGQGNANRTYVTLLTNTFSGPLPSQYPGLSTMYAPIYRIISNARQATDGVVTGTAQV
                     DVLLALVPLTHYAIFYNGLLEFSTCATMVVNGRVHANGSIYTGTSASLTFNGTVTSTG
                     TISSPAWNGQGPRWNHKGTYNGTPPYRTNVPNVSLEIGMTNVHDMLEMPPNGLSPLSY
                     DGRSRFFNKAQTALIITNNSVMMKLQQSINYEVPGADPNPLYLTSNITPTALATNFPF
                     LTTDKTFTDQRENKTVKVTEIDVGRYKQWIQTNVAIQSKFPAANGAYPTILYVADNRT
                     VTSSQMNAVRLTKGDSLPENGGLGWSVATPNPLYMHGHYNCPNAAYRGTTNTTASVPA
                     AIMSDALTILSPTWLDSKSSSRYTTRVPSSTTVNAAILTGTVPSTGPSASQFSGGVHN
                     LPRLLEDWDVGGRVLTLNTAILKLFNSEVATRQFVNPGTYYDPPTRNFSYDLNYLDPY
                     KQPPGIPCAMVLIRLNWAAPPPNTVTYNVAP"
     gene            10493..11095
                     /locus_tag="PKI07_04150"
     CDS             10493..11095
                     /locus_tag="PKI07_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50276.1"
                     /translation="MPALLVLAALLAGNAAAQTLASNPPPAKATMTLRAGEMVGNEQI
                     IRQFIRAGANEFAFVLPAEVRPDETPEGAPVLASRDRSYYLSVRLTTPSRPLPELRQT
                     LKERVASQYPDALNLEDYTAWADGHEGAGFQLQQRQPQVGIRLIRILQVPFKAGVMEF
                     ILNADARNALAGQAALEMVLLTFSSNEHGPLEIIPRSDKT"
     gene            11130..11483
                     /locus_tag="PKI07_04155"
     CDS             11130..11483
                     /locus_tag="PKI07_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50277.1"
                     /translation="MAAIQIQSTKGEPITVVYPAERASAPNTAIQLRIGKKTLLPAYS
                     NANLPHLVQFQRERQVTLFMLADVLETSYWRKLRNAFTIFGQLDMGAGVKVSAGESRR
                     VVQWLRELSAARRPA"
     gene            11504..12646
                     /locus_tag="PKI07_04160"
     CDS             11504..12646
                     /locus_tag="PKI07_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC50278.1"
                     /translation="MQYLRFIGLASLLGFCAAAVAAPAPRITRVILSPRYVENARVPE
                     LTIQSDLAITNQVLYASVPNAQVWTPLTNLLVTHSPYTVIDPQPATAAARFYRVLAQF
                     PVSPPGVAIITAGTFTMGNPISGLYPDELPLHDVHISAFVAEVDLVTKTLWDDVYQWA
                     IAHDYDFANEGDSKEDDHPVVAVSWFDALKWCNARSEKEGLEPAYWTDASQTEVFRSG
                     QINLDSAAVKWNAGYRLPTEAEWEKAARGGASGYNYPWHDSNQFFHTRANVSQNPIFD
                     TGFYPHTSPAGYFPPNDYGLHDMAGNVWEWCWDWYDANWYNEPAASDDDTRGPDWGEY
                     RILRGGSWNDDSTYARCATRGFDDPSASFNAYGFRCVKGHVPAAKP"
     gene            13847..15475
                     /locus_tag="PKI07_04165"
     CDS             13847..15475
                     /locus_tag="PKI07_04165"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOC50279.1"
                     /translation="MNASLPLFIGLVFLLLPSGVRSAPTAAAAPRPNIAVILVDDMGF
                     SDIGCYGGEIPTPHLDQLAARGLRFTQFYNTGRCCPTRASLLTGLYPHQAGVGHMVTD
                     RQQPGYRGNLSDRCVTIAEVLSAAGYRTAMAGKWHVTRYTEPASEAEKANWPRQRGFE
                     HYFGVIKGTANYFRPDSLTRENDPITPPAEGFYTTDAFVDNALQFIDDGDRSKPFFLY
                     LAFNAPHFPLMAPAEEIARFRGKYRVGWDKLRAQRHARQIELGLVDKAWPLAPRPPEV
                     KAWDSLTPVEQDRFDHIMAIYAAVVAHMDTAVGRFVDGLRQRGVLENTLILFLSDNGG
                     NAEAGPDGRCVGQHPGSEDSRVFCGQSWATLENTPFRRYKHFNHEGGIATPLIVHWPA
                     RIARGGQFRTQPGHLIDIMATCVDVAGAAYPKEFRGQAIPPMEGRSLLPAFENKPIQR
                     DALFWEHEGNAAVRVGDWKLVRLGRDGPWELYDLKSDRTEQRNRAAAQPKRVQELAAK
                     WEAWAERAQVKPYPAGDPTDTGVPKKGKKKRAVK"
     gene            15593..17959
                     /locus_tag="PKI07_04170"
     CDS             15593..17959
                     /locus_tag="PKI07_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOC50280.1"
                     /translation="MRIFTHHYDRARSRWITWRAELGGVFLAAFLLAPVVSAQTNAEF
                     RAFWADAFHDGFKTPAQVTKLVADTRAANCNAIVIQVRKRGDAYYESNYEPKATDISP
                     TFDPLQDLINKAHDTNGGPRIEVHAWVVAYTLWNMKNTLPPQANHPYRLHPDWVTQDN
                     TGEAWNGTNYELDPSHPEVQQHLFNVAMDIVSRYDVDGLNWDHIRYEGNTWGYHPLAV
                     ARFRAQYGGSGAPAPTDPAWLQFRRDQVTALVRKSYLGGIALKPWIKMSTCCNTRAPG
                     ITTTAEWPTTASYRNTLQDWRAWLEEGIVDMATPMTYFVASSWGSAWAKWTTFQKDHR
                     YNRQVVVGPGIYLNTVSNSLYQLHNARTPSPSGNYLDGICMYSYATTSSDNVSAAAFR
                     NALVAPSAYDPNPVPVFATPVSPPVMPWKANPTQGHLKGFVYSGPSGAPLDGAVLRLT
                     GPQSRTLTNDGTGFYGAVDLPPGNYLVSAEFAGYGRLTNAVTITAGQVATLDFTLGAH
                     TLRFHSSSLATNQFHSTLSGHGSDAIVVFASTNFAGWRPIRVLPPTNSLLPFCDPDAG
                     SFPQRYYRAGAGPLQTLADFEAYAAGTSVMFRDPAFSGSTSSHLDTNPGVANFTRVTN
                     NFPAGHASARVLQASWTFKSGTANPWLRLTTSSAPNLPNPVIGFNQVLRFDLYTDRDL
                     YVAIGLRETNTGAPIGGDGGTSGAIEWVGGTTNNSISPPRGRLVTAGQWTTLEFFIPY
                     EPVRASTGNGLLESTTGKGVLEQLTLVPADGVGAYNLYLDNFQVLDLE"
     gene            18127..20592
                     /locus_tag="PKI07_04175"
     CDS             18127..20592
                     /locus_tag="PKI07_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50281.1"
                     /translation="MSLGLALLAGTLPLSARVSAPPLSLQRNVQKLAAVPQLILPATD
                     VAKELAAEQQKAAPGPLRFAVARPVAVTTAKQGAWEQLADGRLWRLRIVSAGATDLNL
                     GFSTFWLPEGATLHLLSESEPSFQGPYTARDHQPDGQLWTAVVPGEAAVLELFVPAHA
                     PQEPQLLLAQVSTGYRNFAHLWKDMGRPKSAGTCNNDVVCPVALPWTNEIRSVAVYTE
                     SGIWSCTGTLIMSAAGDFRPFFLTANHCDLNADNAGSMVVYWNYQSATCGTHGPGSLA
                     QNQSGAIFRAAKQEVDFALVELSSLPDPSFQVYYSGWDRSGVAPAGCVGIHHPDSDVK
                     SISFSTTPLTTVDSCIATGGHNTHWHVLWSSGVTEPGSSGSGIWDTSTHRLVGTLSGG
                     NSQCGGWNQTDCYGKFSVAWNSGSSALDRLRDWLDPLETAGLSLPGADPMLTPVLVLT
                     DVALAAENCSPTNGVIDSGETVTLNFTLKNFGGRSTTNLVVSLLAAPGIILPSAPHYC
                     GALASGGSVTCSFSFTACGPCGGAIHPVLQLQDGAHQYPNAAYVMGNGPVSFTLGVPT
                     ASEYYAENFDQVTAPDLPANWTTSSYAWVTSATRRDSFPNAAFAPNSNKVTDYQLVSP
                     PISIPSAQTQLTFRHYYDTEDDYDGGVLEIALNGGVFTDILSAGGTFLNGGYNGSISS
                     SYSNPLGGRAAWTGFSDWTTTTVQLPAAAGDSVQLRWRFGSDSSYGGIGWYVDSITLS
                     AFSYDCCGMPLLLVNPRLVPPDGFAFSYYALTGYNYVVEAQTYAGLSNWIPLQTNAGD
                     CQLQSYTNSTLAAPQLFFRVRRQ"
     gene            20707..21885
                     /locus_tag="PKI07_04180"
     CDS             20707..21885
                     /locus_tag="PKI07_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HOC50282.1"
                     /translation="MATQRQPRPGTLAVWAGEKKDAWWGATQVPVAHSVAFGYANVDE
                     WLAAARGRRPGHIYSRNTNPTVAAFENKVRQLEGAEAATSFASGMAAISNTLFTLLAP
                     GDRLVSVKDSYGGTNRLFLDFLPRYQIEVRLCDTTDHRAIEAEVAKGCRVLYLETPTN
                     PTLKVMDLARLARAAHKVGATVVADNTFATPLNQTPLALGVDLVLHSATKFLGGHADA
                     LGGVVCGPAKLVRAIHHHREITGAALDPMAAYLLLRGMKTLHLRISQQNESALQIARF
                     LATQPQVNQVFYPGLPSHPQHDIARRQMRGFGGVLSFMLKGGFKAVRAFLPRLQYAHL
                     AANLGAVETIAGPPATSSHVECTPAERKAMGIPEGLIRYSVGIEDAEDLIADLKQAFK
                     "
     gene            22158..22427
                     /gene="rpmB"
                     /locus_tag="PKI07_04185"
     CDS             22158..22427
                     /gene="rpmB"
                     /locus_tag="PKI07_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOC50283.1"
                     /translation="MARICELTGKRPIKGSIIWRSGKSKKSGGIGTHVTAITKRRFMP
                     NLQRVKAVVDGEVRYMRVSAKAIKKGLIVKAPKRTWKKEAPAVKA"
     gene            complement(22457..24205)
                     /locus_tag="PKI07_04190"
     CDS             complement(22457..24205)
                     /locus_tag="PKI07_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOC50284.1"
                     /translation="MNQNPDSGPKEAGDLLWEQGKLTEAQLEQVRRRQRRLNLPQHRA
                     IVDLNFASEEDTWRALAAVRHLEFVDPFALGLKRDILELVPIKLIFHYHLLPVRLEED
                     CLTLAFSEPPTQIEQGNLRLLLSKRFKIALATPSSIHAVIKKNFGLGAETIQKLREDR
                     GGAEISQEITFDLQGKETDSAIEATVSAFVDQILQEALRLKATDIHLEPYANSVRLRY
                     RVDGILETVPVPADMRQLQPAVVSRLKIMAGLNIAEKRLPHDGRIAMKTGQEEYDLRV
                     SIMPAKYGEAVCLRILGRQNLFLDLSHLGMEPSQEAVLAQLTQLPQGMVLLTGPTGSG
                     KTTTLYAALAQANDEGRKIITFEDPIEYQLEGTVQIQVREQIGLTFAAGLRSVLRHDP
                     DVVLVGEIRDFETAEIAVRAAQTGHLVFSTLHTNDSVSAVTRLLEMRIEPYLIASSLV
                     CSIAQRLARRICRHCAEEDHSIADATRKEMAAALSLSPNEVKAWKGRGCVECNHKGHR
                     GRVAIYEFFLLNEAIMDLIQPGLRTGELRKAARELGWRSLREMAWRKVQEGLIPISEQ
                     ERWTRVIDPAALLPKA"
     gene            complement(24202..24708)
                     /locus_tag="PKI07_04195"
     CDS             complement(24202..24708)
                     /locus_tag="PKI07_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50285.1"
                     /translation="MKQALRSRWFAACAHVGLWLLLILALVNLGGQAPELHDTDAVTT
                     PLQDTVPVAGLVNLFSPEAEPGPLNVTNTLNPFFTRHFIPKAVPPPTTRKIEVTYLGF
                     YQTGNDPKRTLFKLGAAYQVAPIGARIATNLLIAEATMQAMTLTNLAAQTNILPLNVK
                     KELEVPIQ"
     gene            complement(24728..25549)
                     /locus_tag="PKI07_04200"
     CDS             complement(24728..25549)
                     /locus_tag="PKI07_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50286.1"
                     /translation="MRIPFKEYQRRAVIPVAGLVLAGYYLFFMRPLAQRAENLEKPLQ
                     EAWHQLLVSLDQTNAKTVDFRHITNQLSETRQALQVLDNARQQAIGRLQLAPLVRARI
                     QNDFQLVDYENERGKQLEDLSKLAKQQKVTIAPAVFGGFPEHTADVKQPALLWAALAL
                     TDGLLRTAIQCQLTAIHSLGVTPVLTNAAPTNSFERLVAMPVQIEFTSSAANAGTFLQ
                     CLPLRAEEIQAAGLLKAPVEKPVLFIDRLLVQKQSPEKPDDVHVWLRVVGLVLRE"
     gene            complement(25546..27396)
                     /gene="pilM"
                     /locus_tag="PKI07_04205"
     CDS             complement(25546..27396)
                     /gene="pilM"
                     /locus_tag="PKI07_04205"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOC50287.1"
                     /translation="MTKLNRRQPPMRRVLALDAGSRCIKLLLAESDFGRLRILKEELV
                     DLQAEGLVAAEELQTHLQATLEEWGRPPLALILPQHASVSQVIDLPLAPESEVARLIQ
                     DETVKLSGVSESRIVYDFVRTDTAAQGRQQFWVTWAKEGDIRDRIARLGVEQEDLCEV
                     TTTANALIAAYRLANAQSSRAILVHLGAQTTVLVILLAGQGVFATSFQMGGDFFTRSL
                     ARLRSCPEETAETQKRTQDVLNGPDACSDFAGVVDGWVAELKRQLNEWFEHNAELAPD
                     AAAFELVACGGGFSQPGLLEYLRARAGMDFQLWPQDTQFETVQPSKQFEVAFGAALQA
                     LGYSTQPVSLLPEDYRLAWRKRQSRQRLEVASLGLVVLCLLAFAFGTWRQTSIIDQKR
                     ALLGKLQCARGAVEANNALTAELYRDYESFRPMFACQQNTLDTLKTLTLLQQTRSNRS
                     FWYVLFADQQSYFNQPAGLSLTNTLSATNVFATIAGHGKSPGGATLERSASAGTSTNL
                     SPAKPGFIAELCIPDGGDEARRVRRELVDELKQQRLFSKVDLLSDDLRRNLAESKVVI
                     SDRNIVLALDFAATEFQQPLPPRKPPPSLPARAVGGRALQNGDNLHPIAP"
     gene            complement(27433..29256)
                     /locus_tag="PKI07_04210"
     CDS             complement(27433..29256)
                     /locus_tag="PKI07_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HOC50288.1"
                     /translation="MLDLAGKGRWEEAQTKADALREMAPNNSLVERLHGWVLQAAHQR
                     REQALENDIREIDAKNSVFSPTISSLLKENRDRGLPAKKDVRDAVDRIENALWVPETY
                     GKTVREKGPLFEFESEKGRMVKVLEKEVTVHLDNVPLETMLINLSQTAGVNIVADKSL
                     AALKQSLSVNLDKVRLGEFLRYVGRNYELQFQVGDDLVWVVDAKDPKRLMEETRFYRL
                     RKGFVLPAEFGAPEATRTKTTAGATVTTTEVQKLQRFVNDQAPDLPALERAITNLFTG
                     SKYMIDYERNLVVARGTPEQLSVMENIIKEFDRPIQQVLIEARFITVSKPAFLQLGVL
                     WQTGPRGATNVMPQDFTGLVNNRNFPHSLSASGSVNPAVGKGISEVFTNVLGASDLSA
                     TISVLEQSGESQTLSAPRLTVLNNRPAFVSDGLVQYYYEEYSVSATVQQYFSASSIYP
                     SGKPSKITAGAELNVLASVSGDGESILLALNPKVNTEVLLQTYTTVSQVNAQGNATSS
                     FDIKLPTYRTQELSTRVAIKSGETVVMGGVLEREKSTYVESVPVLGDIPILGAFFRRR
                     TERDIPRYLLVFVTATIVKDTGEFLVYDNDNSATDATAVSK"
     gene            complement(29485..29940)
                     /gene="smpB"
                     /locus_tag="PKI07_04215"
     CDS             complement(29485..29940)
                     /gene="smpB"
                     /locus_tag="PKI07_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOC50289.1"
                     /translation="MADILTNPKARRDFHILEIFEVGIVLRGTEVKALRAGKGQISDA
                     FARVENNEAWLYNAHIDEYSHGNLLNHLPKAPRKLLLHKPEIRKLFGLAAIKGNALVP
                     LAFYWKNGKVKVALAVGKGKVQYDKREDLKRREADRELKRATMRRFKRN"
     gene            complement(30031..30285)
                     /locus_tag="PKI07_04220"
     CDS             complement(30031..30285)
                     /locus_tag="PKI07_04220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOC50290.1"
                     /translation="MNAQLVKMALEKVGNPNLLVNLISRRVRQLSSGGGKMGRPLLAD
                     VGQLGAADIALREIAEDKIGYEMPEWSALVRPGPKKRGRR"
     gene            30414..31328
                     /locus_tag="PKI07_04225"
     CDS             30414..31328
                     /locus_tag="PKI07_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOC50291.1"
                     /translation="MKRPGSVAAQAFNLPEYLATRVAFVDRALDLFLPAATAKPATIH
                     RAMRYSIFAGGKRIRPALCLAAAVAAGGDEAEALPLACAVECIHTYSLVHDDLPAMDD
                     DDYRRGKLTSHKVFGEGIAVLAGDALLTQAFEIAAGCRGWPRYSHQTIILELARAAGS
                     LQLIAGQVADLESEGKRISAQQLRYIHERKTSALLACSVRLGGMSANCTPAQLQALTT
                     FGSNVGLAFQVIDDILDVTQTSEKLGKTAGKDPRAQKATYPALLGLDRSRRIADQLTR
                     RAFAALRGFKGHARALAALAEFLLRRDH"
     gene            31647..32705
                     /locus_tag="PKI07_04230"
     CDS             31647..32705
                     /locus_tag="PKI07_04230"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOC50292.1"
                     /translation="MKSYNIAVIGGDGTGPEVVRETIKVLNVAAKKFNLKLNYTDYDL
                     GGDRYLRTKEALPDSVLAELRKFPAILLGAIGHPDVQPGILEKGILLRIRFELEQYIN
                     LRPVKLYDERFCPLKNKKPEHIDFVVVRENNEGLYTGSGGFTYKGSPHEVALQASIST
                     RPGVERCLRYAFDLTRKRNKEKKLTLCGKTNVLTFAFDLWERAFHEIGQKDYPDIKRD
                     YAHVDATTMWFVKNPEWFDVIVTDNMFGDIITDLGAMVQGGMGIAAGGNINPKGTSMF
                     EPIGGSAPKYTGQGVINPLAAICAGQMMLDFLGETKAAQAIENAVIKITREKIKDLAA
                     GKMGFNTSQVGDLVAGAL"
     gene            complement(32915..34339)
                     /gene="gatB"
                     /locus_tag="PKI07_04235"
     CDS             complement(32915..34339)
                     /gene="gatB"
                     /locus_tag="PKI07_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOC50293.1"
                     /translation="MEYEAVIGLETHVQLKTKSKMWCGCANEFGAPPNTHVCPVCLGL
                     PGVLPVANEEALRLTALSGFLLNCTVNRYVKFDRKNYFYPDVSKNYQLTQHEHPSTRN
                     GCVEFEFQGGLARVRIIQAHLEEDVGKNFHFDRESGVDFNRAGVPLMEIVTEPDITSA
                     EMAYEYLNALKDILVYGGVSDCDMEKGMVRCDVNVSVRPRGQDRLGARVEIKNMNSFS
                     GVRKALEYEIPRQVKVLEQGGQLIQSTRRWNDVAGITEEMRTKEHVHDYRYFPEPDLM
                     PFQLSDAWLAKVKSRVVELPLARKQRFMRDYQLPAPDAQTFVWDVPLGNYFEGIARQA
                     RNPKAAANWVINNLRAQLTEAGTTLADLKFPPTGILELIDLVDSGKISSRIAQEVFGE
                     MFATGQSPGQIVEKKGLVQVSDAGALEQFCDQAIAAHPGPVADFKAGKAAALNFLKGQ
                     VMKLSKGKANPALVGEILERKLKG"
     gene            34647..36314
                     /gene="leuA"
                     /locus_tag="PKI07_04240"
     CDS             34647..36314
                     /gene="leuA"
                     /locus_tag="PKI07_04240"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOC50294.1"
                     /translation="MLKDPSTKYRPFPAVRLPDRQWPNRTLTHAPMWCSVDLRDGNQA
                     LAIPMNVSQKLELFQLLVKCGFKEIEVGFPSASNTEFTFNRRLIEEQRVPEDVWLQVL
                     VQAREDLIERTVESLVGAKKAIIHLYNSTSPAQRRIVFGMSREEILGIAVRGAQWVRE
                     RLSQLKGTEVRLQYSPESFSATEVEFAKEVAEAVMDVWKPTPQHKMILNLPDTVEVTT
                     PNVYADQVEWTCRNIKNRDSLIVSLHTHNDRATGVAATELGMLAGADRVEGTLFGNGE
                     RTGNLDIVTVALNLYMQGIDPKLDFSDLNALAEVYERSTGMRIPPRHPYAGELVFTAF
                     SGSHQDAIKKGLAEWATGRQKYWEVPYLTLDPADIGRQYREVIRVNSQSGKGGVAYLL
                     ESEYGIDLPKEMQREFGPIANEEVDRLGREVTGAELKSMFWREYIERTSPWQLQGFET
                     ESRNGVVRCRARLLREGEPVELSGEGNGPLAALVRGFSALGLPRFEIVSYREHALSAG
                     EEAAAIAYIQIKHSDGRTHWGAGVDTNIELASVRAVLSALNRSQASK"
     gene            complement(37071..37304)
                     /locus_tag="PKI07_04245"
     CDS             complement(37071..37304)
                     /locus_tag="PKI07_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOC50295.1"
                     /translation="MPRFSPEHWRNLEAVFVAAGFRFARQEGSHRSYVKEGVARPVVI
                     PVYREVPVFIIRNNLKTANLSRDDYFRLLSQVK"
     gene            complement(37315..37584)
                     /locus_tag="PKI07_04250"
     CDS             complement(37315..37584)
                     /locus_tag="PKI07_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50296.1"
                     /translation="MKIDYTVQIWREGAQFIAHAMPLDLASSGSTPERARQAVDEAVR
                     LFLATAAEHGTLNEVLEDAGYCREGDSWRGPDWLGVEQRSCLAAA"
     gene            complement(37722..38207)
                     /locus_tag="PKI07_04255"
     CDS             complement(37722..38207)
                     /locus_tag="PKI07_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50297.1"
                     /translation="MKNAAITSLCIGTLAVGLIVGAGIGAWWSGRVFTRMMYAKPEVD
                     QAFLAAQEAEWAARLRLNEPENTLRDLENSISFRLATIATWETVAPADEQTRKARDRF
                     LTAVKVYGQSYPLGGPDAAGIETLLAAVPGRSSASACKNGVCRLDDLQIARAGTTTNT
                     P"
BASE COUNT         7747 a        12186 c        11125 g         7604 t
ORIGIN      
        1 ccagtggctt accatgaccc gctgcttttt gcagaacttt taggacacta ccgccaaaag
       61 gcgtccggac actcgatctg ccctgaaaaa cagccctttg ccagccttag cttcataact
      121 agctgttttt acggagctta ataattatcc ccccatcctc gctcatcgtt tcggcctttc
      181 caggcatcca tagccattgt ccggatgtct tggaaatacc ctgctcctgc gccgtgtaca
      241 aggggtgtac aagggatgct catcgggtgt accagctaaa atcccatgga ctcccggtga
      301 gcatcccttg tacacccctt gtacacggcc taatggcagg gtgaatccag ggaatgtcca
      361 gggtgcgccg aagccggcag gatttcgatt ttgcctcaat tttacccctc tcgatgctta
      421 aactcttaat tttacctgcg aaagatccgg gattccgcag ccatgccatt ggattcgcga
      481 aacaagtggc tcgccggagc tacggtttgg acgttcaggc ccgtccagga tccgatggcc
      541 cgagggcggg cggttggacg gtccaaatga atcgttcaga tttagttgac aatcggcgtg
      601 ctattgtgta taaacacagc aattgctaga tacatgaaag ccacgaagct gccgggattt
      661 atagtcgctc tgatcagtgc gcttgccgcg atgtcggctg ccgctcaaat catgatcgag
      721 acggtgccga tcggcaatgt tgggaacccc aacgatgcca ccggccttgg cagcgtggcg
      781 tattcttatc aaatcggcaa atacgaggtg acgctgaatc agtacaccgc attcctcaac
      841 gcggtggggg caacggacac ctacgggctt tacaatcccc tgatgggcag ttttgcgcag
      901 tccctgggca tcgcgcgcag tgggacagcc gggagttata catattcggt aatcgggagc
      961 ggggagcggc cggtgacgtg ggtgagctgg tttgacgcgg cgcgcttcgt aaactggctg
     1021 gagaatggcc agccgacggg ggcgcaggat gcctcgacga ccgaacgcgg cgcctatacg
     1081 ctggatggcg ccctgagcgg cgtgaacttc acgcgcaatg cgaatgcgac ctacgggttg
     1141 ccgaccgaaa acgaatggta taaggcggcc tattatcagc cggcggacca gggtggcgat
     1201 agcgatgact actggctcta tccaacgcgc agcaacctcc agcccaacag ccgcaatggc
     1261 agcgccaccg atccgaacag tgccaattac ttttataatg atggcatccc caacggctac
     1321 aacggcggct ttgcgatcaa taattccaca acctacccga ccggcaacgc gctgactgac
     1381 gtgggcgcct tttcgctggc cagcagctat tacggcactt tcgaccaggg cggcaacgtg
     1441 tcggaatgga acgacacggt catcgaggct tttgcttacc gcgcctaccg cggcggttcc
     1501 tggttctatg gcgtaggcaa cttgcgcgcg gatagtgacc tcaacggcct ggccccggac
     1561 caggagcggg aaaccctggg gttccggatc gtcgtcatcc ccgagccttc ggcggttggg
     1621 ttgatggttc tgggcgcggc cctgttgctc ggacggcgga aacgcgcttg ttgacgcagc
     1681 gcgcgccggt tcccgaagca gcccggggcg gccccggcaa tgccgcgctg gacgttagcc
     1741 ctgccggggg ctgctgcaag gattgcgaac cgtatttccc atccccgccc acccgcggca
     1801 cgctaacttc cgcaaaacac cgggctttcg aaacgccgcg ctggcggggg ctgccgcgtc
     1861 aatcgccaag cccgggaaga acggcccggc gggatgatgg cggaaaggcg cgggagccgg
     1921 ttcatcgtga aaagccgaag ccgctgctta aaggaaagcg cccgcggcgc ggcagccgga
     1981 ctgcggccgc gatgtccggc gcggttgcga taatgtattt gcggattgtc tattgcgcgg
     2041 gagctgtttt atgctggcgc gcatcaatgg ttgtttgccg ccacctggga catgactcca
     2101 accttgctga tagtagatga tgagaagacg acgcgcgacg gattgcgcgc ggcgctcgag
     2161 catcgctacg acgtgtacct ggccgaggat gccaaggcgg cgatggagtt attggaggcg
     2221 gagcattttg acgtgttgct gactgatttc cggctgccga ccgaagacgg gatgaagctg
     2281 attatgcgcg ccaagtcgct gtcccggccg cccatctgca tcctgatgac cgcttatggg
     2341 tcggaggaac tggccgtgga ggcgatgaag catggggccg acgattacat tgccaagggg
     2401 cggctgcaac tggacgagct ggagatgcgg atcaccaacg cgttgcggcg gcagcggctc
     2461 gaaacggaaa acgcggccct gcgccagcaa ctcgacacgc ggttcggctt gcaaaacctg
     2521 atcggggagt ccccgggcat gaaagaggtt ttcgagatgg tgcagcaggt ggcgcccacg
     2581 cgggcgaccg tgctgttgct gggggaaagc gggacgggca aggaaatggt cgccaaggcc
     2641 atccaccagc tcagcccgcg cgcgcagcat cccctggtga cggtgcattg cgcggcgctc
     2701 tcgccgacgc tgctggagag cgagctcttc ggccacgaga aaggcgcctt tacgggcgcg
     2761 tatgagcggc gcctgggccg gttcgagcag gcgcagagcg gcacgctgtt tctggacgaa
     2821 atcggcgaaa ttgacgccac catccaaatc aaattgctgc ggttcctggg cgagcgaacc
     2881 ttcgagcggg tcggatcgaa taagacgctc agcgcggatg tgcgcctgat cgccgcgacc
     2941 aacaagaacc tggaagcgca agtcaaggcg ggcgcgtttc gcgaggatct ctacttccgc
     3001 ctgcgggtgg tggagttgcg gctgccgcct ttgcgcgaac gcctggagga tattcccctg
     3061 ctggcgcggg cgtttcagag tgaattcagc cgggaaaatg agaaaccgtt gaaaggcttt
     3121 accaccgagg cctttgatgc gttgatgcgg tacccgtggc cggggaacgt gcgggaactg
     3181 cgcaccgcca tcgagcacgc cgtcatcctg tgccggggcg agaaaatcac cctgcgcgac
     3241 ctgcccctgg cgctgcgcga ggcggtcagc cggacgccgg acgaaaagga cattaagcgg
     3301 ctgctcgcgc gccaggacct gacgagcaag gagatcgagc gggagcgcat catcagcgcc
     3361 ctgaaggcaa cccaggggaa ccgcacccag gcggcccggc gattgggcat gagccgccgc
     3421 aattttcacc gcaaattgca tctgcaccgc atcgaagaaa tctgaatatg ccgttcttac
     3481 aagacttgct gcataaactg gagatcggcg gcggcatgcg ctatttgcgc atcctgccgg
     3541 ccctgatgac catcgttctg ctgggggtga tttataattg gcgcggctac aagaatatgg
     3601 ccacccagga agcgatggac gcggcgcaag tggcgcgcaa cctggccgaa ggcaagggct
     3661 atacgacgct tttcatccgg cccttcagca tctacctgct taaaaagcgc gcccaggaaa
     3721 aaggggaaat gcccaagctc ggggaagtcg ccgacccggc ccatctcaaa ggaatgcacc
     3781 cggacctggc caacccgccc ggctacccgg tggtgctggc gggactgctg aagttgttgc
     3841 gcccggatta cgccgtgtcc accacccagc ctttctggag cgatagcggg cggttctggc
     3901 gctataaacc ggacttcctg attaccctgt tcaatcagct cctgttcctg gcggccatcc
     3961 cggtggtgtt ttttctggcc cgccacctgt ttgactccgg ggtggcctgg ctctcggcgg
     4021 tgctgttgct ggggacggaa ctgttctggc ggttcagcgt atcgggactg tccacgctgc
     4081 tgctgctgct cattttcctg gggctggtct ggagcatcgt gctgcttgaa cacgaagtgc
     4141 gcgctccccg ctgggggcgg tatggtgtat tcattctggc cgggctggcc ggtgcaatga
     4201 ccggcatcgg tgggctgacg cgctacgcgt ttggctggct catccttccc gtcctggtat
     4261 tcctgattct gtatggcggc cagcggcgaa tcgtgctgac cctgaccact ctggcggtat
     4321 ttgcggccat catgacaccg tggatggctc ggaactatga cctcagcgga gctccctttg
     4381 gcactgccac gtactccgtc gtggaaacca ccatgctgtc ccctgaatac cggctccaac
     4441 gctccctgga gccggacttc agccgcctct acctgatggc cttctggctc aaactcaaca
     4501 acaacctccg ccaaatcgtc accagcgatc taccccggct cgggggcagt tgggttagcg
     4561 ccttctttct ggtggggctg ctcattggct ttaataatcc ttccactcaa cgcatgcgct
     4621 acttcctgct ggggtgcgtg gtggtgctat gcctggcgca ggctttgggg cgcactcagc
     4681 tttcggaaga cgtccccgag atcaattccg agaacctgct agtgctggtc gcgcccctgg
     4741 tgttgattta cggcgtcagc cttttctttc tgctgctgga gcgaatcccg ttgccgttcc
     4801 cccagatgcg ctatctcgtg atggccctct tcagcctggc cgtctgcctg ccgatgttgt
     4861 tcgttttcct gccgcccaag acgaatcccg tcgcctaccc gccctactac ccgcccgcca
     4921 tccagacgat cgccggctgg atgaaggaaa aggagctggt gatgagcgat atcccctggg
     4981 ccgtggcctg gtacggtcag cggcaatgtg tctggctgac gctaaaatgc actgcggact
     5041 ctcgggacgc gggctccggc gagagtttct ttgccattaa cgattattac aaacccatta
     5101 atgccctcta cctgaccccg cgaaccatgg acgcccgctt cctgagccag tggatccgtg
     5161 cgggggatca gagctggggc aatttcatcc tggaaagcct gctgcagaag agggtcccgg
     5221 gatatttccc gctcagcgaa tcgcaatccg gctggctgcc cgaacaattg gtgctgacgg
     5281 actggcaacg ctggcgaaaa ccgtaggctc ctttttctgg acgcggttga cagccgcgaa
     5341 ccgaaccctg atgataaacc catgagcgag atcatctatc ctcgaagtcc ccgcgaagta
     5401 atgtgcggct ggatgcacct gccgcggttt attgacaaga cccggctcca cctggccggt
     5461 aagctgcatc cggactacca gcccaacttc tgcaaaggct tcgacgccct atggctggaa
     5521 accgccggcg tcgaagccca acagttcatc gaggtggtgc gcgcccgaat cacggatggc
     5581 gaggtctgcg actgggtgcg ccagaatgtg ttaaagcccg aatcggtcaa agccgcgcac
     5641 cgcgagcaaa tgctcaacta tccccgaaag gatgatgccc aaatgcaggc gctcctgaaa
     5701 ttgcgcaaag aacaggccgg actcgcccac cgcgaggaca ttcagaactt tgtggatttc
     5761 attgacgccg acgaaaagcg gatttagcag ctacgccagc ctgcggcacc gccccggcac
     5821 ccgtcaatcc gtggcgaaag cgccgttagc ctgccaccgc gcgccgaccc gaccggcgcg
     5881 gcccctgccc cgggcaatca ctgtcccagc ctcggccgat aacgccatag ccaccgtccc
     5941 cggcttgcgc tcctcctcaa gtttccgcac tctgaacccg ttatgatcct ctgcctcgga
     6001 accaccccgg cggcgcaacg ggtgatggtc tttcaccggc tcacgctgga cgccgtaaat
     6061 cgcgccgtga caaccctcga cggcgcggcg ggcaaatcgg tcaatgtcgc caagacgctg
     6121 aaggcgttgg gcgaacaacc ggtcgccgtt ggtttcctcg gcggcgaccg cggggcggaa
     6181 gtgcgggcat tgctggccgc gcagaacatc gcggcggatt tcgtcaacgt ctccgcccgc
     6241 acgcgccagt gcaccacgct ccttgaccag gccgccggca ccattaccga actggtcgaa
     6301 gaaggccaac cggtatcaac ggcggattat gccgcgctcc tggacatcct ccgacgccgc
     6361 gcccgcggcg cccgcgccat tgtgatgtcg ggcagcatca ccccgggcgg gccagccgat
     6421 ctttactttc acggcaccca aatcgcccgc gcaaacgggg cgctggcgat cgtggacgcg
     6481 cagggtgccg cgcttgccga ggcgctcaag gccggcccgg acctggtaaa acccaaccgg
     6541 ctggaactgg cggccaccct ggggcgggaa ctccccgacg aagccgccgt catcgccggc
     6601 atgcaggaac tgtgcaagcg cggcgcgctc cgcgtggttg tcacatccgg ccagggccca
     6661 acgctggcgt atgatggcca aaacggctgg aaaatccaag ccccgcaggt taagacgacg
     6721 aatcccatcg gctcgggcga tgccttcacg gccggtttgg cctggcggtt ggtgcgaggg
     6781 gaaaaccttg gtgaagcctg ccgctggggc gccgccgccg gcaccgccaa cgccctgacg
     6841 ttaatggccg gcgaagtcca ccgcgccgac gtcgagcgcc tggcggcgca agtgtcggta
     6901 gaaccgctgc cagcccgctc gcccggcttt ttttgaataa agggcgcagg agcaccgaaa
     6961 gacttggcgt ggcgaggcgg gctactcaat cacggcttgc cagttaagga aaaccattgg
     7021 cggaccggcg cagcctggat acgccgtccg gccggcatca accattgacc ggcaaatctg
     7081 cggggtgtct tattttaagg cagccatttc tcgtactgga cagacgctcg ctgcggtccg
     7141 ggttggcccg ggcgatgggt ttggtgtcgg cctcactttt cccgcgctga aaacacacac
     7201 cttcgccgcc gtcattcagt aaacgggcag cacccggttc ggggcggaat agcgcaaacc
     7261 ggcaccattc atgctgcccg gcaagtacga tgaattgccg cgcggctaac aggatgaagg
     7321 tgaccagtgc agccggcccg ggtagagcgg gccgtgcgca cccctttgcg cccgctccgg
     7381 cttaagagaa acaagttgcc catgccacac gtctccgaac tacaacggaa gcctgcgaat
     7441 cacgccggcg caggattaat cggcggcgcg aaccgcgccc agcccttgtg gatagcgccc
     7501 gcgagggcgg cgctgcgggg cggaaagcga atcccggcct cgcggggatt tactttgacc
     7561 gaggtcgtaa tctccctggc aattatcgcc ctggtcttcg gcggcatatt gatggcctac
     7621 gtccaggccg cccgaaaagc cgagtggtcc ggctattcgc tggcggcgca agccatcggg
     7681 attcaccaaa tcgagcaggc tcgctcggcc gtatgggatt attcgatcga caagaacgaa
     7741 ctgaccaacc tgaatctcaa tgggtggagc tataataaaa tcacccgaac cggcaaaggc
     7801 tggacatctc gggtgctgga tattccccgg tcgggaacga acgtcgtggt cgcgacgaat
     7861 ttcgtaaccg tgaaactgct gagcgtgacg ggtttgacca acgtgcatgt gcatatggtg
     7921 atggtggaca cggtttggcg cttcccgatg ggaaacagca cgcgattgtt caccaatcag
     7981 acggcgagct atttcgggcc ggacaaccgc gatgtgagca gcttatgaag atgcccgcaa
     8041 tctggagggg aacggccgag ccgccgcggc gccggcgggc cgggttcacg ttgtcggagc
     8101 tgctggtctc ttcggcggtc ttcagcctgg cgataatggg attcgtttac tgccagatgt
     8161 tcgggatgcg gcaggaccag ttggtcaaca gcaagatcgg ggcaaccgag ctcgcccgcc
     8221 tgagcttcaa tgagctgtgc aacgacatcc gcgccgccaa ggtatggcag attggcaacg
     8281 gcagtctggg ttcgtttacc cccatcccgc tgggcgacaa tcaaaagggg aacgcgctca
     8341 agctgagcat gacgatcaat accaacagct actacctgta ttacttcgac accaacgcct
     8401 gcaagctcta ccgcggccac agcgggagcg ctgccctcaa gtgcatggcg gcatacctga
     8461 ccaataccat gtatttccag gccacggatt atcggggcga gaaccagacg gacctgaccc
     8521 acaaaggcgt aatcaacgtg gtgatgcagt ttcatcagta ccaatacccc atcacgaaga
     8581 tcggccccgg ccagctttac gattattacc ggatagaatt acaggcgacc ccccacgtgc
     8641 cggacgggcc ctgaccccac gaaaaggacg ctcatgaata tgcaaacaaa tgccacccgc
     8701 gcgcgccgca acggctatgc gctgctgatg gtgttgacgg ccaccggcat tgcgtcgctg
     8761 gtgctggcgg gcaccttgaa ccgttcgttg accaacgcca agatgaacga ccgcagcaac
     8821 gagtacagtg tggcgtccaa cgcggcggaa gccgccgtgg aaaaagtggt ggcccgcatg
     8881 gggcacgact tccagaacta cggcccgggc gcggttgcgg cgaacatcaa tctctaccgg
     8941 aacagcatcc cggacgagga ttccttctgg acgcaattcg cgttctccga tggccagggg
     9001 aatgccaatc gcacctacgt gacgctgctc accaacacct tctccgggcc cctgccgtcc
     9061 cagtatcccg gcttgagcac catgtatgcc cccatctacc gcatcatatc caatgcgcgg
     9121 caggcaaccg acggcgtggt gaccggcacc gcgcaggtgg atgtcctgct ggccctggtg
     9181 ccgctgaccc actacgcaat cttctacaac gggttgctgg aattcagcac gtgcgccacg
     9241 atggtcgtga acggccgggt ccatgccaac ggctccatct acaccggcac cagcgcatcg
     9301 ctcaccttca acggcaccgt gaccagcacc ggcaccattt cttcacccgc ttggaacgga
     9361 caggggccca gatggaatca taaaggcacc tacaacggca ccccgcccta tcgcaccaac
     9421 gtgcccaatg tcagcctgga gattggcatg accaatgtgc atgacatgct agagatgccg
     9481 ccgaacgggc tgagcccatt gagctacgat gggcgatccc gcttctttaa caaggcccag
     9541 accgcgctga tcatcaccaa caacagtgtg atgatgaagc tgcagcagag catcaactat
     9601 gaagtgcccg gggccgatcc caacccgctg tatctgacct cgaatattac cccaacggcg
     9661 ctggcaacca actttccgtt cctgacgacg gacaaaacct tcaccgacca gcgggagaac
     9721 aagaccgtca aggtgaccga aattgatgtg gggagataca aacaatggat ccagaccaac
     9781 gtggccattc agagcaaatt tcccgccgct aatggcgcct acccaaccat cctttacgtg
     9841 gcggataacc gcaccgtcac ctcctcgcaa atgaacgccg tgcggctgac caaaggcgac
     9901 agcttgcccg aaaatggcgg tttgggctgg agcgtcgcca cccccaaccc tctctacatg
     9961 cacgggcatt ataattgccc caacgccgcc tatcgcggca ccaccaatac gaccgcctcg
    10021 gtgcctgccg ccatcatgag cgacgcgctg accatcctct ctcccacctg gctggacagc
    10081 aagagcagca gcagatacac gacgcgcgtc cccagcagca ccactgtgaa tgcggccatt
    10141 ctgacgggaa ccgtgccctc caccggccca tccgcttcgc agttcagcgg cggagtgcat
    10201 aacctgcccc ggctgctgga agactgggat gtcggcggcc gcgtcctgac gctcaacacc
    10261 gccattttga agctgttcaa cagtgaagtg gcaacccgcc agtttgttaa tcccggcacc
    10321 tattacgatc cgcccacccg caatttcagc tacgatctga actacctgga cccctacaag
    10381 cagccgcccg gcattccttg cgcgatggtc ctgatccgcc tcaactgggc ggcgccgccg
    10441 cccaacaccg tcacctacaa tgtcgctccc taaactaccg cactgcagcc cagtgcccgc
    10501 tctcctggtc ttggccgccc tgctggcggg gaacgccgcc gcccaaactc tggccagcaa
    10561 tcccccgccc gccaaggcaa ccatgacgct gcgggcgggg gaaatggtgg ggaatgagca
    10621 aatcatccgg cagttcatcc gggcgggggc gaacgaattt gccttcgtgc tgccggccga
    10681 agtgcgcccg gacgagacgc cggagggcgc gccagttctg gcgagccggg atcggagcta
    10741 ttatctgtcg gtccggctca ccacaccctc ccgcccgctg ccagaactgc ggcaaacgct
    10801 gaaagaaagg gtcgccagcc agtatccgga cgccctcaac ctggaagatt acaccgcctg
    10861 ggcggacggg catgagggcg ccgggtttca gttgcaacag cggcagccgc aagtgggcat
    10921 ccgtctgatc cgtattttgc aggtgccctt taaggcgggc gtaatggagt ttatcttaaa
    10981 cgcggatgcc cggaacgccc tcgccggcca ggcggccctg gaaatggtcc tgctgacttt
    11041 tagctcgaac gagcatggcc cgctggagat catcccgcgc tcggacaaaa cctgatcccc
    11101 ccacgcccac ttacaaaccc gctttcgcta tggctgccat tcaaattcaa agcacaaaag
    11161 gcgaacccat cacggtggtt tatccggcgg aaagagcctc cgcgccgaac accgccatac
    11221 agttgcggat aggcaaaaaa accctgttgc ccgcctattc caacgccaac ctgccgcacc
    11281 tggtgcaatt ccagcgagaa cggcaggtca ccctgttcat gctggcggat gtgctcgaaa
    11341 cctcctactg gcgcaagctg cgcaacgctt tcacgatctt tgggcagttg gatatgggcg
    11401 cgggcgttaa agtcagcgcg ggcgaatccc ggcgggtagt acaatggctg cgcgaactca
    11461 gcgcggcccg ccgaccggct taaccgaccc ttacgttctc aatatgcaat accttcgttt
    11521 tattggacta gccagcctgc tgggattctg cgccgccgcc gttgcggcac ccgccccgcg
    11581 catcacccgg gtgatcctgt ccccccggta cgttgaaaat gcccgggtgc ccgagcttac
    11641 catccaaagc gatttggcga tcaccaacca ggtcctttac gcgagtgtcc ccaacgcgca
    11701 agtctggacg cccctgacca atctgctggt cacacactcg ccttatacgg tgatcgaccc
    11761 ccaacccgcg acggccgccg cgcgttttta tcgcgtcctg gctcaatttc ccgtctcgcc
    11821 tcccggcgtg gcgattatca cggccggcac ctttaccatg ggaaatccga tctccggcct
    11881 ctatcccgac gaactgccgc tccacgacgt ccatatcagc gcattcgtgg cagaggtgga
    11941 cctcgtcacc aaaacgctgt gggatgacgt ctatcaatgg gcgatcgccc atgattacga
    12001 ctttgccaat gaaggcgata gcaaggagga cgaccacccg gtcgtggcgg tcagttggtt
    12061 tgacgccctg aagtggtgca acgcccgttc ggaaaaagag ggactggaac cggcctactg
    12121 gaccgatgcg agtcaaaccg aagtcttccg ctccggccag ataaatctgg atagcgcggc
    12181 ggtgaaatgg aatgccggct accgtttgcc cacggaagct gaatgggaaa aggccgcccg
    12241 cggcggcgca tcgggctaca actacccgtg gcacgacagc aaccagttct tccacacccg
    12301 cgccaatgtg agccaaaacc ccatattcga tactggcttt tatccgcaca ccagcccggc
    12361 aggctacttc ccgcccaatg attatgggtt gcacgatatg gccggaaacg tctgggagtg
    12421 gtgctgggac tggtatgacg ccaactggta caacgaaccc gctgcttccg acgacgacac
    12481 ccgcggcccg gactgggggg aataccgcat tctgcgaggc ggatcatgga acgatgattc
    12541 cacttatgcc cggtgcgcca cgcgcggctt tgacgaccca tccgcctcct tcaacgccta
    12601 cggcttccgt tgcgtaaaag gccacgtccc cgccgccaaa ccttaaccgg agcgatgcaa
    12661 gtgggccgcc agcccgggct cccaccttct ctttgatccg ggataggtcc ggccacccgg
    12721 gcctcaacct caacaaatcc ccaggctggt agtttccacg aggcagccct ttggctccaa
    12781 acggtttacg gcagtaaaac cagcagttta cacacgcaaa gagggcgaaa tagagacgaa
    12841 atcggactca ttccgacata agctcaccat ggacgtccct tggatatacc ctgcttctac
    12901 gtcgtgtaca aggggtgtac aagggatgct catcgggtat accagctaaa atcctatgga
    12961 cctccggtga gcatccgttg tacacccctt gtacacggcg taggagtggg gtaagtttaa
    13021 ggggcctggg tgatggtttt ggatgcccga taaggccgaa acgatgatat ggaacggaaa
    13081 ggcgatttct aacctactgg aaagcggtta gttataaata tatgaccgat gggaggcagt
    13141 tttccaagag aggctgaggg gttggttgcc tatcggtttt gcaggtattt tagccgaaac
    13201 aatcctggct ctacctccac tccgcccctc cgcatgtgta taaaccattg gttttatagt
    13261 cgaaagctat catggattta aattccaggc agttgaatcc gctaaccaga aagcccgtta
    13321 aggctgcaac ctgaacgggc aagtcctccc ggcctccgat ggaacggtat cgcatgacag
    13381 ggcttcctaa ctggatcgct tcgacttgag cgtctgcgag catagcgacg cacggtcccg
    13441 caggaggcct aacgttcggg ctctcttcta ttttgggcgg cgccccaacg ctgggacggg
    13501 tgcatcctgg tttttggctc caaagcgttg atatatgcga aaacggcccc cgtttctacg
    13561 aaagcaagcc gtaatctcga gcttcctttt ccctgccgaa gcccaaccgg ctttgccgga
    13621 agcgctgtcc cactttttat cccgggctgg gccaaccgca tcgcctgccc gccgacgcta
    13681 ccctcttccg cggcctctgc ttctcatggg gcgctgttgc gtaattgctt cgtaggcgcc
    13741 ctggcttcaa taaaggcgcg ctccctattg cgatagtccg gggaccaacc ggcggttagc
    13801 aatttgccgt attgccaacc cgccgggtgc ctcgcactat ggaggcatga acgcatcgct
    13861 tccgctcttc atcgggcttg ttttcctgtt gctgccgtcc ggagtgcgca gtgcccccac
    13921 cgccgccgcc gcgccgcgcc cgaatatcgc ggtcattctg gtggatgata tggggttctc
    13981 ggatatcggt tgttatggcg gggaaattcc cactccccac ctggatcagt tggcggcgcg
    14041 cgggttgcgg ttcacacaat tctataatac gggccgctgc tgccccaccc gcgcttcgct
    14101 gctgaccggg ttgtatccgc accaggccgg cgtcggccac atggtcaccg accggcagca
    14161 accgggctac cgggggaacc tcagcgaccg ctgcgtgacc attgccgaag tcctgagcgc
    14221 cgcggggtat cgcaccgcca tggccggcaa gtggcatgtg acgcggtaca ccgaacccgc
    14281 cagcgaggcg gagaaagcca actggccccg gcagcgcgga ttcgagcatt actttggcgt
    14341 gattaaaggc acggccaact acttccgccc cgactcatta acccgcgaga atgaccccat
    14401 tacccctccg gcggagggtt tctacactac cgatgctttt gtggacaacg cccttcaatt
    14461 cattgatgac ggggaccggt caaagccctt cttcctctac ctggccttca atgcgccgca
    14521 tttcccgctc atggcgccgg cggaggaaat cgccaggttc cgcggcaaat accgggttgg
    14581 ctgggacaaa ctgcgcgcgc agcggcacgc ccggcagatc gagttgggcc tggtggacaa
    14641 agcctggccg ctggctccgc gcccgccgga ggtcaaggcc tgggacagcc tgacgcccgt
    14701 cgagcaggat cggttcgacc acatcatggc catctacgct gccgtggtgg cacacatgga
    14761 caccgccgtg ggccggttcg tggacggcct gcgccagcgc ggcgtactcg agaatacgct
    14821 gatcctcttt ctctcggaca acggcggcaa tgccgaagcc ggcccggacg gccgttgcgt
    14881 cggccagcat cccggctcgg aagattcccg cgtcttttgc ggccaatcct gggctacgct
    14941 cgaaaacacg cccttccggc gctataagca tttcaatcat gaaggcggca tcgcaacgcc
    15001 gctcattgtg cattggccgg cacggatcgc gcgcggcgga cagtttcgca cccaaccggg
    15061 tcatttgatt gacatcatgg ccacttgcgt ggacgtggcc ggtgccgcgt atccgaaaga
    15121 gttccggggc caggcgattc cgccgatgga aggccgcagc ttgctgccgg cgttcgaaaa
    15181 taagcctatt caacgcgacg ccctcttttg ggagcatgag ggcaacgcgg cggtgcgggt
    15241 gggggactgg aaactggtcc gcctcggccg cgacggcccg tgggagcttt acgacctgaa
    15301 aagcgatcgc accgaacaac gcaaccgggc ggcagcgcag cccaaacggg ttcaggaact
    15361 ggccgccaaa tgggaggcct gggccgaacg cgcccaggtg aagccctatc ctgccggaga
    15421 cccaactgac accggcgtgc ccaagaaagg caaaaagaaa cgcgccgtca aatagcggcg
    15481 gggccgggat tcggatatcg tgaggcggac cgggagccgg cactcccggc gcagggcgac
    15541 ggcggaagcc gccgtacttg cacgtaagcc ggtttcaagg catactgctc taatgcggat
    15601 ctttacgcac cactatgacc gggcgcgttc gcgctggatt acctggcgcg ctgaactggg
    15661 cggcgtgttt ctggccgcct ttcttctggc ccccgtcgtc tccgcccaaa ccaacgccga
    15721 gtttcgcgcc ttctgggccg acgcattcca cgatggattc aaaacgccgg cccaagtgac
    15781 caagctggtg gccgatacgc gcgcggctaa ttgcaatgcc atcgttattc aggtgcgcaa
    15841 acgcggggat gcctactacg agtcgaacta tgaacccaaa gccacggata tctcccccac
    15901 ctttgatccg ttgcaggacc tgatcaacaa ggcccacgat accaacgggg gcccccgcat
    15961 cgaggtgcat gcctgggtgg tcgcgtatac cctctggaat atgaaaaaca cgctgccgcc
    16021 ccaggctaat cacccctacc ggcttcatcc cgattgggtg acgcaggaca acaccggcga
    16081 ggcctggaac ggcaccaact acgaactcga ccccagccat cctgaggtgc agcagcacct
    16141 cttcaacgtc gcgatggaca ttgtgtcccg ctacgacgtg gatgggctga actgggacca
    16201 tatccgctat gaaggcaata cctggggtta ccatccgctg gccgtggcgc gcttccgcgc
    16261 gcaatacggc ggcagcggcg cgccagcccc caccgacccg gcctggctgc agttccggcg
    16321 ggaccaggtc accgcgctgg tgcgcaagag ttatctcggc ggcatcgcgc tcaagccgtg
    16381 gatcaagatg tccacttgct gcaatacccg cgctccgggc atcaccacga ccgccgaatg
    16441 gcccaccacc gcctcctacc gcaacacgct gcaggattgg cgcgcctggc tcgaagaagg
    16501 gatcgtggat atggcaacgc cgatgaccta tttcgtcgcc agctcctggg gcagcgcctg
    16561 ggcgaagtgg actaccttcc aaaaggatca tcgctacaac cggcaggtcg tggttggccc
    16621 gggcatctac ctgaataccg tctccaattc cctttaccag ttgcacaacg cgcgcacccc
    16681 gagtccgagc ggcaattatc ttgatggaat ttgcatgtat tcctatgcta ccaccagcag
    16741 cgataatgta tcggcggccg ccttccgcaa cgccctggtc gcgcccagcg cctatgaccc
    16801 caacccggtg ccggtttttg cgactcccgt cagcccgccg gtgatgccct ggaaagccaa
    16861 tcccacccaa ggccacctca agggctttgt ttatagcggc ccctccggtg cgccgctgga
    16921 tggcgctgtt ctgcgattga ccgggccgca aagccggacc ttgaccaacg atggcacggg
    16981 cttttacggc gcggtggact tgccgccggg gaactacctc gtcagcgccg aattcgccgg
    17041 ctatggccgg ctgactaatg cggtcaccat caccgccggt caggtggcca cgctggattt
    17101 cacgctgggc gcccacaccc tgcgattcca ctcatccagc cttgccacga accagttcca
    17161 tagcaccctt tccggccacg gctcggacgc gatcgtggtc ttcgcctcga ccaacttcgc
    17221 cggctggcgg ccaatccggg tgctgccccc aaccaattcc ctgctgccct tctgcgaccc
    17281 cgatgccggg agcttcccgc aacgctacta ccgcgcgggc gccggcccgc tgcaaacgct
    17341 ggccgatttc gaagcttatg ccgccgggac cagtgtgatg ttccgggatc cggccttctc
    17401 cggctcgact tcaagccact tggacacaaa ccccggggtt gccaatttca cccgcgtgac
    17461 caataatttc ccggcgggcc acgcgagcgc gcgggtgctt caggcttcct ggaccttcaa
    17521 gagcggcacg gccaacccct ggctgcgcct gaccaccagc agcgctccca acctgcccaa
    17581 tccggtcatc ggctttaacc aggtgctgcg attcgacctt tacaccgacc gggatctcta
    17641 cgtggccatt ggcctgcggg aaaccaacac cggcgcgccg attggcggtg atggcggcac
    17701 ctcgggtgca attgaatggg tcggcggcac gacgaataat tccatcagcc cgccccgtgg
    17761 ccgcctggtc accgcaggac aatggaccac cctcgagttc ttcatcccgt atgaaccggt
    17821 gcgagcctcc acgggcaacg gcctcctgga gtccaccacg ggcaagggag tgctcgaaca
    17881 attaaccctg gtgcctgccg acggcgtggg cgcctataat ctctacctgg acaacttcca
    17941 ggttctggac ttggaatagg aatacccctc ttgcggaacc atgctttccg gcacatcggc
    18001 gcccgtgctt cttgcgcgcc cgctgccctg ccccgcactg gattctgcgc tcacccgctg
    18061 ctcccagccc tcatgaaaac ccgttgtccc tgctccctgg ttcgtctttc cccccccggc
    18121 tctcttttga gccttgggct cgcgctgctg gccggcacgc ttccgctttc ggcgcgtgtc
    18181 agcgcgccgc cgctcagtct gcagcggaac gtccagaaac tggccgccgt cccgcaactc
    18241 atcctgccag ccaccgacgt cgcgaaggaa ttggccgccg agcagcaaaa ggcggccccc
    18301 ggcccgctgc gctttgccgt ggcgcgcccg gtggcggtaa ccacggccaa acagggggcc
    18361 tgggaacaac tggcggatgg ccggctctgg cgcttgcgga ttgtctcagc gggcgccacc
    18421 gacctgaacc tgggcttctc cactttctgg ctgcccgaag gcgccactct ccatctgctt
    18481 tcggaaagcg aaccttcttt ccagggcccc tacaccgccc gggaccacca gcctgacggt
    18541 cagctttgga cggcagtcgt gcccggggaa gcggcggtgc tcgaactgtt cgtccccgcg
    18601 cacgcgccgc aggagcctca gctcttgctg gcccaggtca gcaccggcta ccggaatttc
    18661 gcccacctat ggaaggatat gggccgaccc aaaagcgcag gcacctgcaa caacgacgtc
    18721 gtctgccccg ttgcccttcc ctggaccaac gagattcgaa gcgtcgccgt ttataccgaa
    18781 agtggaatct ggagctgcac cggcaccctc atcatgtcgg cggctggcga ttttcgtcct
    18841 tttttcctga ccgccaatca ctgtgacctg aacgccgata atgcaggctc gatggtggtg
    18901 tactggaact accaatcggc aacctgcggg acgcatgggc ccggctcgct cgcgcagaat
    18961 caaagcgggg cgatctttcg cgcggccaaa caagaggtgg atttcgcgct cgtcgagttg
    19021 tcatccctcc ctgatcccag cttccaggta tattactcgg gctgggatcg ttccggcgtg
    19081 gctcccgcgg ggtgcgtcgg tatccaccac cccgatagtg atgtgaaatc catcagcttt
    19141 tcgaccaccc ccctgaccac cgttgatagt tgcatcgcca cgggcggcca taatactcac
    19201 tggcacgtgc tgtggagttc gggcgtcacc gaacccggct cttccggctc cggaatatgg
    19261 gacacctcga cccatcggct ggtgggcact ctttccggcg gcaactccca atgcggcggt
    19321 tggaaccaga cggattgcta cggcaagttc tcggtcgcct ggaacagcgg ctcttccgcc
    19381 ttagaccgtt tgcgcgactg gctggacccc ctcgaaaccg cggggctcag cctgccgggc
    19441 gccgatccga tgttgacccc ggtcctcgtg ctgaccgatg tggcgctggc cgccgaaaac
    19501 tgttccccaa ccaacggggt gattgattcc ggcgaaacgg tcaccttgaa ttttacgctt
    19561 aaaaacttcg gcggccgaag tacgactaat ctcgttgtct cgctgctggc ggccccgggc
    19621 attatccttc ccagcgcgcc gcactattgc ggtgccctcg ccagcggcgg atcggtgacc
    19681 tgctccttct ctttcaccgc ttgcggcccc tgcggcggcg ccatccaccc tgtcctgcaa
    19741 ctccaggacg gagcccacca ataccccaac gccgcctacg tgatgggcaa cggtcccgtg
    19801 agtttcacac tgggggtccc gaccgcgagc gaatactatg ccgaaaactt cgaccaagtg
    19861 acggctcctg atctgccagc caactggact acttccagtt atgcctgggt aacctcggcc
    19921 acgcggcggg attctttccc gaatgccgcc tttgcgccaa atagcaacaa agtgaccgac
    19981 taccaactcg tttctccgcc catctccatc ccctcagctc agacccaact gacattccgg
    20041 cactattatg acaccgagga tgactatgac ggcggagttc tggagatcgc cctcaacggc
    20101 ggcgtattta ccgatattct tagcgcgggc ggcacgtttc tcaacggcgg atacaacggc
    20161 tctatttcct ccagctacag taatccgctg ggcggacggg ccgcctggac ggggttctcg
    20221 gattggacga ccactacggt gcaattgcct gccgccgccg gcgacagcgt ccagttgcgc
    20281 tggcgatttg gctccgacag cagctatggc ggcatcggct ggtatgtgga ctcgattaca
    20341 ctctccgctt tcagctacga ctgctgcggc atgccgctgt tgctggtgaa tcctcggctc
    20401 gtcccccccg acggctttgc cttctcctac tacgcgctca ccggctataa ctacgtggta
    20461 gaagcccaaa cctatgctgg cctctcgaac tggattccgc tgcaaaccaa tgccggcgat
    20521 tgccagttgc aatcctatac caactccact ctcgccgccc cgcaactctt cttccgggtg
    20581 cgccggcaat aagccctcgg ggcaactcac ggccacgcga tagcggagcg caaactcgcc
    20641 cgccaccgac cgctgcccgc gaatgccagg ttgcacgtgc ccctcgcgca tggtatctac
    20701 tgaagcatgg caactcaacg tcagcctcgc cccggcaccc tggccgtatg ggcgggagaa
    20761 aagaaagatg cttggtgggg agccacgcag gtgccggtcg cgcacagcgt cgcctttggc
    20821 tatgcgaacg tggatgaatg gctggccgcc gcgcgggggc gtcgccccgg ccatatttac
    20881 agccggaata ccaacccgac ggtcgccgcc ttcgaaaaca aagtgcgcca actggagggg
    20941 gccgaagccg ccaccagctt tgcctccggc atggctgcca tcagcaacac cctgttcact
    21001 ctgttggcgc ctggggaccg cctggtctcg gtcaaggaca gctacggtgg caccaaccgg
    21061 ctgttcctgg acttccttcc tcgctatcag attgaagtcc ggctgtgcga caccacggat
    21121 catcgagcta ttgaggctga ggtggcgaaa ggctgccgcg tgctctatct ggaaacgccg
    21181 accaacccca ccctcaaagt gatggacctg gcgcgcctgg cgcgggccgc gcataaagtc
    21241 ggcgccaccg tggtggccga caacaccttt gccacgccgc tcaaccaaac cccgctggcg
    21301 ttgggggtgg atctcgtttt gcacagcgca accaagttcc tgggcggcca cgccgatgct
    21361 ctgggaggcg ttgtgtgcgg accggcgaaa ctggtccgcg cgatccacca tcaccgtgaa
    21421 atcaccggcg cggcgctcga cccgatggcc gcctacctcc tgctgcgggg aatgaagacc
    21481 ctgcacctgc gcatcagcca acaaaacgaa agcgccttgc aaattgcccg gttcctggca
    21541 actcaaccac aggtgaacca ggttttttat cccgggctgc cttcgcatcc acagcatgat
    21601 attgcccggc gtcagatgcg cggtttcggc ggtgtgctga gtttcatgct caaaggcggc
    21661 ttcaaagccg tccgcgcctt cctcccccgg ctgcaatatg cgcacttggc cgccaacctg
    21721 ggagccgtcg aaaccatcgc cgggccgccc gctacctcca gccatgtcga atgcacgccg
    21781 gctgagcgca aggcaatggg catccctgaa ggcttaatcc gctattcggt cggcattgag
    21841 gacgccgaag atctgatcgc cgacctgaag caggccttca aataatccac ggccaacgaa
    21901 gtctctgggc gggacataag ggtgggatga gttcccgttt gggctagccc ggctccggcg
    21961 ggcggtgctt ttcggaggtt gaaaacccgt gtgcggtttg ttttcgagat aacaaaaacc
    22021 gggcctccgc tccctgcttc tttgggatat tgcctcaaac cggggagccg ttcttttgcc
    22081 ttctgctgtc gatactgctt gcagagcggc ggaagcctgc taatatgccg gacagttgat
    22141 attttaagag gaattgtatg gcgagaattt gtgaattgac cggcaagcgc cccatcaagg
    22201 gcagcatcat ttggcgcagc ggcaaatcga agaagagtgg cggcattggc acgcatgtta
    22261 cggctattac taaacgccgg ttcatgccga atttgcagcg ggtcaaagcc gtggtggatg
    22321 gcgaggtgcg ttatatgcgc gtttccgcca aggcaattaa gaagggcctc atcgtcaaag
    22381 cccctaagcg cacctggaag aaggaagcgc cggcggtcaa ggcttgatcc ggcgcatatt
    22441 gtcccccctc gcggctttac gctttaggca atagcgcggc gggatctatc acgcgggtcc
    22501 accgctcctg ctcggaaatc ggaatcagcc cctcctgcac cttgcgccag gccatttcgc
    22561 gcagagagcg ccagccgagt tcccgcgcgg ctttgcggag ttccccggtt cgcaagccgg
    22621 gctgaataag gtccataata gcttcgttga ggaggaaaaa ctcgtagatg gccacgcggc
    22681 cccggtggcc cttgtggtta cattcgacac agccgcggcc cttccaggct ttaacttcgt
    22741 taggggacag ggaaagggcg gcggccattt ccttgcgcgt ggcgtccgcg atggagtggt
    22801 cttcctcggc gcagtgacgg caaatgcgcc gcgccagtcg ctgcgcaatg ctgcatacca
    22861 gcgaagatgc gatcaaatat ggttcgatgc gcatctccag caggcgcgtg acggcgctga
    22921 cactgtcgtt ggtgtgcagc gttgagaaaa ccagatggcc ggtctgggcg gcgcggacgg
    22981 caatttcggc ggtctcgaaa tcacgaatct cacccacaag cacgacgtcc gggtcgtggc
    23041 gcaggaccga acgcaatccg gcggcaaagg tcaggccgat ctgttcgcga acctggattt
    23101 gaacggtgcc ttcgagttgg tattcgatgg ggtcctcgaa ggtgatgatc ttgcggcctt
    23161 catcgttggc ctgcgccagc gcggcgtaca gggtggttgt cttgccgctg ccggtggggc
    23221 cggtcagcaa caccataccc tgcggcaact gcgtcaactg ggccagcacg gcttcctggc
    23281 tcggttccat gccgagatga cttagatcca ggaacaggtt ctgccgcccg agaatccgca
    23341 gacagaccgc ttccccgtat ttggcgggca taatcgagac gcgcaagtcg tattcttcct
    23401 gccccgtctt catcgctatg cgcccatcgt ggggcaggcg tttctctgcg atattaagcc
    23461 cggccatgat cttgagccgc gacactaccg ccggttgcaa ttgccgcata tcggctggca
    23521 ccggcacggt ctccagaatg ccgtccacgc gatagcgcag gcggacggaa ttggcgtagg
    23581 gttcaaggtg aatgtcggta gccttgagcc ggagcgcctc ctggaggatt tggtccacaa
    23641 aggccgagac ggtggcttcg atcgccgagt cagtttcctt gccttgcaga tcaaaggtaa
    23701 tctcctggct gatctccgcg ccacctcgat cttcacgcag tttctggata gtctcggcac
    23761 caagaccgaa gttcttttta ataacggcat ggatcgaact gggggtggcg agcgcaatct
    23821 tgaagcgttt gctcaacagg agacgcaagt tgccctgttc gatttgggtt ggcggctcgc
    23881 tgaaggcaag ggtcaggcaa tcctcctcga ggcgcaccgg cagcagatgg tagtgaaaaa
    23941 tgagtttgat gggcaccaat tcgaggatgt cgcgcttgag gcccaacgca aaggggtcca
    24001 caaactccaa atgcctgacg gcggccagcg cgcgccaagt gtcctcttcg gaagcgaagt
    24061 tcaggtccac aatcgcccgg tgctgtggca aattgagccg ccgctggcgg cgtcgaacct
    24121 gctcaagctg cgcttccgta agcttgccct gctcccacag gagatcgcca gcttctttgg
    24181 ggccgctgtc cgggttctgg ttcattggat gggaacctcg agttctttct tcacgttcaa
    24241 gggcaaaatg ttggtctgcg ccgccaggtt cgtcaaggtc atggcctgca tggtagcctc
    24301 agctatgagg aggttggtgg caatgcgagc accgatgggt gccacttggt atgccgctcc
    24361 caatttaaaa agcgtacgtt tgggatcgtt gccggtctgg taaaaaccga ggtaggtcac
    24421 ctcgattttc cgggtggtgg gcggcggcac agctttcgga ataaagtggc gcgtgaagaa
    24481 cggattcagt gtgtttgtaa cattgagcgg gcctggttcg gcttccggcg aaaacaggtt
    24541 gaccaaccct gcgaccggta cggtatcctg aagcggggtg gtgacggcat cagtgtcatg
    24601 cagttccgga gcctggccgc cgaggttgac cagcgccagg atcagcagca gccacaaacc
    24661 cacatgagcg caagcggcga accagcggct tctcaaggct tgtttcacga caggcggcat
    24721 ggctgctcta ctcccgcaat accagcccga ctacccgcaa ccacacatgc acgtcgtctg
    24781 gtttctccgg cgattgtttt tgtaccaaga gccgatcaat gaataacacc ggcttttcca
    24841 ccggagcctt gagcaagccc gctgcctgga tttcttctgc ccgcaagggc aggcattgga
    24901 ggaaagtccc cgcgttggct gccgaactgg tgaactcgat ctgcacaggc attgcgacca
    24961 gtcgttcaaa gctattggtg ggggccgcat tcgtcagcac cggcgttacc ccaagcgaat
    25021 gaatcgccgt gagctggcat tgaatcgccg tccgaagcag cccgtcggtg agtgccagag
    25081 cggcccagag caacgccggt tgtttgacgt cggcagtatg ttcaggaaac cctccgaaga
    25141 cggctggcgc gatcgttacc ttctgctgct tggccagttt gctcaagtct tccagttgtt
    25201 tgccgcgttc gttttcatag tcaacaagct gaaagtcatt ctggatccgg gcacggacga
    25261 gcggggcgag ttgcagacgg ccaatggcct gttgccgagc gttgtccaac acctgcaggg
    25321 cttggcgggt ttcgctgagt tggttggtga tatgccgaaa atccacagtc tttgcattag
    25381 tctgatccag tgaaaccagg agttgatgcc aagcttcctg gagtggcttc tccaggttct
    25441 ccgcccgctg cgccagcggc cgcatgaaga acagataata gcctgccagc actaacccgg
    25501 ctacggggat gaccgcccgc cgttgatatt ctttaaaggg aattctcacg gcgcaattgg
    25561 atggagattg tccccatttt gcagcgcgcg cccaccgact gcccgtgccg gcaaactcgg
    25621 cggaggtttt cgaggcggca acggctgctg gaactccgta gccgcaaagt ccaacgccaa
    25681 cacaatgttc cgatccgaga taaccacctt ggactcggcc aggttccggc gcaaatcatc
    25741 cgagagcaaa tccactttcg agaatagccg ctgctgtttc agctcatcca ctaattctcg
    25801 ccgcacccgg cgcgcttcat cgccgccgtc aggaatacag agctcggcaa taaagccagg
    25861 cttggctggt gaaaggtttg tcgaagttcc cgcggatgcc gatcgctcca gcgtcgcgcc
    25921 gcctgggctc ttgccgtggc cagctatcgt cgcaaataca ttggtagcgg acagcgtatt
    25981 ggtcaaggaa agtccggcag gctggttgaa gtagctctgc tgatcagcaa acagcacgta
    26041 ccagaagctg cggttgctgc gagtctgttg aagcagagtc aatgtcttca gcgtatcgag
    26101 tgtgttctgt tggcaggcaa acatcgggcg gaagctttcg taatcccggt ataattcggc
    26161 ggtcagcgca ttgttggctt ccaccgctcc acgggcgcat tgaagtttgc ctaatagtgc
    26221 tcgtttttgg tctataatcg aagtctgacg ccaggtgccg aacgcaaaag ccagcaggca
    26281 aagcacgacc aaacccaagc tcgccacttc cagtcgttgc cggctttgac gcttccgcca
    26341 ggcaaggcga taatcttcgg gcagcaagga aacgggttgt gtgctataac ccagcgcctg
    26401 aagtgcagcg ccaaaagcaa cctcaaattg cttcgagggc tgcaccgttt cgaattgagt
    26461 gtcctggggc caaagctgga agtccatccc ggcccgcgct cttagatatt cgagtagccc
    26521 cggttggctg aatcctccgc cacaggcgac caattcaaac gcggcggcgt ccggcgcgag
    26581 ttcggcattg tgttcgaacc actcattcag ttgccgcttc aattccgcca cccagccatc
    26641 caccaccccg gcaaagtccg aacacgcatc cgggccgttc aggacatcct gggtgcgttt
    26701 ctgggtttcc gcggtctctt caggacaact tcgcagccgc gcgagggagc gagtgaagaa
    26761 atctcctccc atctggaagc tggtggcaaa gacgccctga cccgccagga gaataaccag
    26821 gacggtggtc tgagcgccca aatgcaccaa aatggcacgg gatgactgcg cattggccag
    26881 gcgataagcg gcgatcaggg cattagcagt cgtagtgacc tcgcacagat cctcctgttc
    26941 gacccctaac cgcgcaatgc gatcgcgaat atccccctcc ttcgcccatg tcacccagaa
    27001 ctgctggcgt ccctgggcgg cggtgtcggt gcggacaaag tcatagacga tccggctttc
    27061 gctcacgcca ctcagcttga ccgtttcgtc ctggatcaac ctggctactt cgctctccgg
    27121 cgccagcggc aggtcaatga cttgggaaac agaggcgtgc tgcggcagga tcaacgccag
    27181 cggcggccgc ccccattctt ccagggtggc ttgcaagtgc gtctggagtt cttccgccgc
    27241 aacgaggccc tcggcctgca agtcaaccag ttcctccttg agaatccgca ggcggccaaa
    27301 gtcgctttcg gccagcaaca acttgatgca gcggctgccg gcatccagcg ccagcacccg
    27361 ccgcatcggt ggctgccttc gattgagttt cgtcataacc tgcgcagagc gtcccggaac
    27421 aactcccctg ctctattttg agaccgccgt tgcatccgtg gcggaattat cgttgtcgta
    27481 aaccaggaat tcgcccgtgt ccttaacaat cgtagccgtc acgaaaacca gcaggtagcg
    27541 cggaatatcc cgctccgtcc ggcgccggaa aaacgcgccc aaaatcggga tatcccccag
    27601 caccggcacg gattcaacgt aggtggactt ctcccgctcc agcactccgc ccatgaccac
    27661 cgtctcacct gatttgatgg ccacgcgcgt ggacagttcc tgcgtgcggt aggtcggaag
    27721 tttgatatcg aaactgctgg tcgcattgcc ctgggcgttg acctggctca ccgtggtgta
    27781 ggtttgcagc aatacctccg tgttcacctt cgggttcaag gccaaaagga tgctttcacc
    27841 gtcgccgctt acgctggcca ggacatttaa ctctgcgccg gccgtaatct tcgacggctt
    27901 gccgctgggg taaatcgagg aggcactgaa atactgctgt acggtggccg aaacgctgta
    27961 ttcctcgtaa tagtactgca ccaagccatc gctgacgaag gcggggcgat tgttgagcac
    28021 cgtcagccgc ggtgcgctca aagtttgact ttccccgctt tgttccaaca cactgatagt
    28081 ggcgctcaga tccgaagctc ccagcacatt agtgaatacc tccgaaatcc ctttccccac
    28141 cgctggattt accgagccag aagcagacag ggaatgaggg aagttccggt tgtttaccaa
    28201 tccggtgaaa tcctgtggca tgacattggt agctcctcgc ggaccagttt gccacagcac
    28261 ccccagttgc agaaatgctg gcttggaaac ggtgataaac cgggcttcaa tcaacacctg
    28321 ctgaatcgga cggtcgaatt ccttgataat gttctccatc acgctcagtt gctccggcgt
    28381 accgcgggcg acgaccaggt ttcgctcata atcaatcata tacttggagc cagtgaacag
    28441 gttggtaatc gcccgctcca gggccgggag gtcgggcgcc tgatcgttga cgaacctctg
    28501 gagcttctga acttcggtgg tggtgaccgt agccccggcg gtggtctttg ttcgggtggc
    28561 ttccggcgca ccgaactccg ccggcaacac aaatcctttg cgcaagcggt aaaagcgggt
    28621 ctcctccatc agtcgtttgg ggtctttggc atccaccacc caaaccagat catctcccac
    28681 ctggaactgc aactcgtagt tgcgaccgac atagcggaga aattctccca accgcacttt
    28741 gtccagattg acgcttaacg attgtttcag tgccgccagc gatttgtccg ctacaatgtt
    28801 cactcccgcc gtctggctga gattgatgag catggtttcg agcgggacat tgtccaggtg
    28861 caccgtcacc tccttctcca acacttttac catccgaccc ttttcgctct cgaactcgaa
    28921 cagcggcccc ttctcacgca ccgtcttgcc gtaagtctcc ggaacccaaa gcgcgttctc
    28981 gatgcgatct accgcatcgc gcacgtcttt cttggcgggc aggccgcgat cccggttctc
    29041 cttcagcaga ctcgaaatgg tggggctgaa gaccgagttc tttgcgtcaa tttcccgaat
    29101 atcgttttcc aacgcctgct cccggcgctg atgcgcagct tggaggaccc agccgtgaag
    29161 ccgctcgacc agcgaattgt tgggtgccat ttcccgtaac gcgtcggcct tggtttgcgc
    29221 ttcctcccag cggcctttgc cggccaagtc caacacttct ttaatctcag aatcgtagga
    29281 ttcactatac cggggcgagc gcggagttgg ctccttggtt ggggaatgtt gacaaccggt
    29341 ggatgaaatc agcagcagcg caaccgccac cgttgccttc gatatagtac ttagccagat
    29401 tcgcggaagc atgggttatg atggacgttt tacccggcca tttaattcat gcgccacaac
    29461 aaaatgcaac cagtttctca cgtgttaatt ccgcttgaac cgccgcatgg tagcccgctt
    29521 gagttcgcgg tcagcttccc gtcgttttaa atcctcgcgt ttatcatact ggactttccc
    29581 cttgcccacc gccaaggcca ccttgacctt cccattcttc caataaaagg ccagcggcac
    29641 cagcgcattg cctttgatcg ccgccaggcc gaacagcttc cggatttccg gtttgtgcaa
    29701 tagcagcttg cgcggcgctt tgggcaggtg attcagcaga tttccgtgag aatactcgtc
    29761 aatgtgcgcg ttgtagagcc acgcctcgtt attctccacc cgcgcgaacg catcactaat
    29821 ctgccccttg cccgcgcgca gagccttgac ctcggtgcca cgcagcacga tcccaacctc
    29881 gaaaatctca aggatatgaa aatcccgccg cgctttgggg ttagtcagaa tatcggccat
    29941 aaaatcaaca caactcactc cgggagcggc gtttccgccg cccctccttc gctggccgat
    30001 tcgtccctcc cgccgcttcc gcggcaaccc ctaacgccgc ccgcgcttct tggggcccgg
    30061 ccgcactaat gccgaccact ccggcatttc ataccctatt ttgtcctcgg cgatttcgcg
    30121 cagcgcaatg tccgccgctc ccagttgtcc cacatccgcc aggagcgggc ggcccatctt
    30181 gcccccaccc gaagagagct ggcgcacgcg ccgggaaatc aggttgacca gaaggttggg
    30241 attacccacc ttctccagtg ccatttttac tagttgagcg ttcaagactt agtgtccaaa
    30301 ataaagcggt gtatgttaac acctgccccc gccaacgcaa tacaaatatt tcaattttga
    30361 atggttgccg ctgcctccgg gtctggctac gataagcacg catgtctggc tctatgaagc
    30421 gtcccggttc tgttgcggct caagcgttca acctgccgga atacctcgcc acgcgcgtcg
    30481 cctttgtgga tcgcgccctc gaccttttcc tgccggccgc gactgccaaa cccgccacca
    30541 tccaccgcgc catgcgctac tcgatctttg ccggcggcaa gcgcattcgc cccgctctgt
    30601 gcctcgccgc cgctgtcgcc gccggcggcg acgaagccga agcgctgccc ttggcgtgcg
    30661 ctgtcgaatg catccacacc tattcgctcg ttcatgacga cctgccagcc atggacgatg
    30721 acgattatcg gcgcggcaaa ctcaccagcc acaaagtatt tggcgaagga attgccgtgc
    30781 tcgccgggga cgccctgcta acccaggcct ttgaaattgc cgccggctgc cggggctggc
    30841 ctcgctattc gcatcagacc attatcttgg aactggcccg cgccgcgggc tcgctccagt
    30901 taatcgccgg acaggtagcc gatttggaaa gcgaaggaaa acgcatttcc gcccaacaac
    30961 ttcgttacat ccatgagcga aaaacctcgg ccctcctcgc ttgttcggtc cgactcgggg
    31021 ggatgagtgc caactgcaca cccgcccaac tgcaagcgct caccaccttc ggctccaatg
    31081 tgggcctggc gttccaagtg attgacgata ttctggacgt aacccaaacg agcgagaaac
    31141 tcggcaagac cgccggcaag gacccccggg cgcaaaaagc cacctatccc gccctcctcg
    31201 gattggaccg ctcacgccga atcgccgacc agttaacccg ccgcgctttc gcagccctcc
    31261 ggggatttaa aggccacgcc agggctctgg cagctctggc cgaattcctt ttgcgccggg
    31321 accactagcc ctgtcataga atgtaggctg gcccaccgat agagccaacc gatccctacc
    31381 cggcccaaag cgctcgcgtg cctgctaaca gcccacgccc cacgcagttt ctacggaggt
    31441 taccgtcgga taccgccgac tttacccggc aaacacgttg aaaatggata tgtgagctaa
    31501 cctttcccac actgacacac taaaaatgcg gcggatgcag gctaagctct ggcaacgcca
    31561 taaaaagtca tggctatatt cttgccttca tgatggaagc gactagtttt tcatttcccg
    31621 ccaaaacgcg ggatatagat aagcatatga aaagttataa tattgcagtc atcggcggcg
    31681 acggaacagg gccagaagtg gtccgcgaaa ctattaaagt cctcaatgtt gccgcgaaaa
    31741 aattcaatct caagctcaac tacaccgact acgacctggg cggcgatcgg tatctgcgca
    31801 cgaaagaagc gctgcccgac agcgtgctgg cagaactgcg gaagttcccg gctattctat
    31861 tgggtgccat cggccacccg gacgtgcagc cgggcatact ggaaaagggc atcctgctgc
    31921 gcatccgctt cgaattggaa caatacatca acctgcggcc ggtcaagctt tacgacgagc
    31981 gcttctgtcc gctcaagaac aagaaacccg aacacattga cttcgtggtt gtgcgtgaaa
    32041 ataatgaagg gctttacacc ggctcgggcg ggttcaccta caaaggctcg ccccacgaag
    32101 ttgcgctgca ggcaagcatc agcactcgtc ccggagtcga gcgctgcttg cgctatgctt
    32161 tcgacctcac ccgcaaacgc aacaaagaaa agaagctgac gctctgcggc aaaaccaacg
    32221 tgctgacctt cgcgttcgat ctctgggagc gcgccttcca cgaaatcgga cagaaagatt
    32281 accctgatat caagcgtgac tacgcgcacg tggacgccac caccatgtgg tttgtcaaga
    32341 atcccgaatg gttcgatgtc atcgtgaccg ataatatgtt cggagacatc atcaccgacc
    32401 tgggcgcgat ggtgcaaggc ggcatgggca ttgccgcagg cggcaacatc aacccgaaag
    32461 gcacgagtat gttcgagccg attggcggca gcgctcccaa atacaccggc cagggagtta
    32521 tcaacccgct ggcggccatc tgcgccggcc aaatgatgct ggattttctg ggagaaacca
    32581 aagccgccca agccatcgaa aacgccgtca tcaaaatcac ccgcgagaag ataaaggacc
    32641 tagccgcggg caagatgggc ttcaacacct ctcaggtcgg agacttggtt gcgggggcgc
    32701 tataaaacca agtcgaggga aagctctgcc cccgtaagcc ctgacatgag cccaaacctc
    32761 ccggggatag ttctctgact atctcccggg agggtgcagt gggtgttatc gcggattgcg
    32821 atgacctgcc tgtggcttta tcccccgaag ctttttgacg cacctacgct gccagttgaa
    32881 gctataacgc aagcaggctt ataaagccgc tcgttcaacc cttgagcttg cgttcgagga
    32941 tttcgccgac aagcgcgggg ttggctttgc ctttactgag cttcatgacc tggcctttga
    33001 ggaagttcaa ggcggcggcc ttgccagcct tgaaatcggc aacggggccg ggatgggcgg
    33061 cgatggcctg atcgcagaac tgttcgaggg cacccgcatc gcttacctgc accaggccct
    33121 tcttctctac aatctggccc ggggactggc cggtggcgaa catttccccg aaaacctcct
    33181 gcgcaatgcg gctgctaatc ttgccactgt ccaccaggtc gattaactcc aggatgccgg
    33241 taggcggaaa tttcaagtcg gccagcgtgg tgcccgcctc ggtgagctga gcgcgcaagt
    33301 tgttgatgac ccagttggcg gcggctttgg ggttcctggc ctgccgggct atgccttcga
    33361 agtaatttcc caggggcacg tcccagacga aagtctgcgc gtcgggggcg ggcagttgat
    33421 agtcacgcat gaagcgttgc ttgcgggcca atggcaactc caccacccgg ctcttcacct
    33481 tagccagcca cgcatcgctt agctggaaag gcatcaagtc cggttcggga aaatagcggt
    33541 aatcatgcac gtgctccttg gtgcgcattt cctcggtgat gcctgccaca tcgttccaac
    33601 ggcgggtaga ttggatgagt tgcccgcctt gttcgagcac cttgacctgg cgcggaattt
    33661 cgtattccaa cgctttgcgc acgccgctga aactgttcat gttcttgatt tcgacccggg
    33721 cgccaagccg gtcctggcca cggggacgca cgctcacgtt aacgtcgcag cgcaccatgc
    33781 ctttctccat atcgcaatcg ctcacgccgc catagaccag gatgtcttta agggcgttaa
    33841 ggtattcgta ggccatttcc gcactggtaa tgtccggctc ggtaacaatc tccatcagcg
    33901 gcacgcccgc gcggttaaag tccaccccgc tttcgcggtc aaagtgaaag tttttgccaa
    33961 catcctcctc aaggtgggcc tggatgatgc gcaccctcgc aaggccgccc tgaaactcga
    34021 actcgacgca gccatttcgg gtcgaggggt gctcatgctg ggtgagttgg tagtttttgg
    34081 aaacgtccgg gtaaaaatag ttcttccggt cgaacttaac atagcggttc acggtgcagt
    34141 tgagcaggaa cccgctcagc gcagtcaggc gcagcgcctc ttcgttggcg acgggcaaga
    34201 cgccgggcag gcccagacac accgggcaaa catgggtgtt gggcggagcg ccgaactcgt
    34261 tggcgcagcc gcaccacatc ttggactttg tcttaagctg cacgtgggtt tccagcccaa
    34321 taaccgcttc gtattccata atgccgggat agttaccaca aaaggccccg ggaagacacg
    34381 gataattctt gcccgctaaa cccaaatttc ggctcttgcg acggcttcct gaaaaaacac
    34441 ttgcgtccgg cggggaaagc ggttaagttg cggggcatga atggcaattt aaacctgctg
    34501 cttaagagcg cgctgctgct gtgccgcgcg gcagtcgggt tgtagccttt cataaaagct
    34561 tacgaacccc cactgccgca gccggtggtg ggggtttttt atttccccgg accacccggc
    34621 ggtgttaaca agaaagcgat acaactatgc tgaaagatcc gtccaccaaa taccgtccgt
    34681 tcccggcggt ccgtttgcct gaccgccagt ggcccaaccg taccctcacc catgcgccca
    34741 tgtggtgcag cgtggacctg cgggatggaa accaggcgct ggcgatcccc atgaacgtca
    34801 gccagaagct tgagctgttc cagttgctgg tgaagtgcgg cttcaaggaa atcgaagtag
    34861 gcttcccgtc cgcctcgaac accgagttca cattcaaccg ccgcctgatc gaggagcagc
    34921 gcgtgccgga agacgtgtgg ctccaggtgt tggtgcaggc gcgcgaggat ttgatcgagc
    34981 gcaccgtcga atcgctggtc ggcgctaaaa aggccatcat ccacctttac aactcaacct
    35041 ccccggcgca gcgccggatc gtgttcggca tgtcccggga ggaaatcctc gggattgccg
    35101 tgcgcggtgc ccagtgggtc cgcgagcgcc tgtcgcaact caagggcacg gaagtccggc
    35161 tccagtattc gccggagagc ttcagcgcca ccgaagtcga gttcgccaaa gaagtcgccg
    35221 aagcggtcat ggacgtgtgg aaacccactc cgcagcacaa gatgattctc aacctgcccg
    35281 acacggtgga ggtgaccacg cccaacgtct atgcggacca ggtcgagtgg acctgccgca
    35341 acatcaaaaa ccgcgattcg ctgattgtga gcctgcacac ccataacgac cgggccacgg
    35401 gcgtggccgc caccgaactg ggaatgctgg ccggcgccga ccgcgtcgaa ggcacgctct
    35461 tcggcaacgg cgagcgaacg ggcaacctcg acatcgtgac cgtcgccctg aacctttaca
    35521 tgcagggcat tgatccaaag ctcgattttt ccgatctcaa cgctctcgcc gaagtctatg
    35581 agcgcagcac gggcatgcgg attcccccgc gccaccccta cgccggtgag ttggtcttca
    35641 ccgccttcag cggctcgcat caggacgcca ttaaaaaggg cctggccgaa tgggcaaccg
    35701 ggcgtcaaaa atactgggaa gtgccctatc tcacccttga tcccgccgac attggccgcc
    35761 agtaccgcga ggtcatccgc gtcaacagcc agtccggcaa aggcggcgtc gcctacttgt
    35821 tggagagtga atacggcatt gacctcccca aagaaatgca gcgcgagttc ggccccatcg
    35881 ccaacgaaga ggtggaccgc ctgggccgcg aggtcaccgg cgccgagctc aagagcatgt
    35941 tctggcgcga atacatcgag cgcacctcgc cctggcagct tcagggattc gagaccgaaa
    36001 gccgcaacgg cgtggtccgg tgccgcgccc gcctgctccg tgagggggaa ccggtcgaac
    36061 tcagcggcga aggcaacggc ccgctggccg cgctggtccg cggcttcagc gcgctcggcc
    36121 tcccgcgatt cgagatcgtg agctaccgtg agcacgctct gagcgccggc gaagaagccg
    36181 ccgccatcgc ctacatccaa atcaagcaca gcgatggacg cacccattgg ggcgccggcg
    36241 tggataccaa catcgaattg gcctcggtcc gcgccgtcct cagcgctctg aaccgctcgc
    36301 aggccagcaa ataaactgcc ggcgctcgtg ctgccggggt tcgggcgcct ggctgccgct
    36361 ccacacggaa ccaggcgccc tcggatgtcg gtcgggggct ttggttttgt agatcataaa
    36421 tcgggaacag agcggaagcg ttcgccttgg ccgcccacgc aggggacagg agcgcgaatc
    36481 ggcggccagg acaaaagcgg agcggagtga tggcgctcaa aaagcttatc gagggagacc
    36541 ctgcgggggg gccagtcctg ccggtttcac ggcgtagctc ttttaagggg aagcgaaagc
    36601 cggcaagtct ggcaacgggg atcatgccag ctttcgggcc agaccttgtc cgcaggcagg
    36661 attatctagg gaacagacaa cttaaagttc ggatgttctg cgcgaacctt ttaaactgat
    36721 actgatcgga atttgctttt gcgcacctcg cacattcagc cggatggcgt cctggcggag
    36781 aggaagttgt tctgtgaact gggctcaaac gggataacca tcgtcaaccg agacacatct
    36841 atcccccccc ggcaggggcg tgtcggtcta tgatccgacg agcaggagat tggacagata
    36901 gacatgccgg aaaatggact gtaaacatgt gcattggcgg caagggggaa agatgctgtt
    36961 caccaccgcc atcgagggcc tttatgcgct gaagatggct gtccatgcgt ggacagccaa
    37021 tgcgagactc catacgcccg ggggcatcca gcgcgacgcc agtgaatgcc tcacttcacc
    37081 tgggagagca gtctgaaata gtcgtcgcgc gataggttag cggtcttcaa gttgtttcgg
    37141 atgatgaaga ctggcacctc ccggtaaaca ggaataacca ccggccgcgc cacgccttct
    37201 ttcacgtagg acctatggct gccctcctga cgagcaaaac ggaaaccggc ggccacgaac
    37261 acggcttcca ggttgcgcca gtgctctgga ctgaatcgcg gcatggaaac gggactatgc
    37321 cgccgccaga caacttcgtt gctcgactcc gagccaatcc ggccctcgcc aggagtcgcc
    37381 ttcgcggcag tacccggcat cttccaagac ctcattgaga gtgccgtgtt ctgccgcggt
    37441 ggcgaggaac aagcgcaccg cctcgtccac tgcctggcgg gccctttcgg gtgtgctacc
    37501 cgaactggcc aagtccaggg gcatcgcatg cgcaatgaac tgcgctccct cgcgccagat
    37561 ttgcacggta taatcaatct tcatgcctta gcctaacatt cttgcctttc gttcacaacg
    37621 ccagttcgcc ggctttcatg ccgcctgccg ctggtctttg atgctgcccg ttaaaatttg
    37681 ctttcccccc gcccacccgt gttcaacgca gcacgggtgt atcacggggt attggtcgtc
    37741 gtgcctgctc tcgctatctg caaatcatcg agccgacaaa caccattctt gcaggcgctc
    37801 gccgaactcc gccccgggac tgcggccaac agcgtttcga tgcctgcggc gtcggggccg
    37861 ccaaggggat aactctgccc gtaaaccttc actgccgtca aaaatctatc gcgcgccttc
    37921 cgcgtctgtt cgtctgccgg tgcgacggtc tcccaagtcg caatcgtcgc caaacggaag
    37981 gaaatggaat tctctaaatc tcgaagggtg ttctctggct cgttcaagcg aaggcgggct
    38041 gcccactctg cctcttgcgc cgccaaaaat gcctgatcca cttcgggctt ggcgtacatc
    38101 atcctggtga atacacgccc agaccaccac gcgccgatcc cagcgccgac tataaggcca
    38161 accgccagcg tcccgatgca caagcttgta atagcggcat ttttcatggc gtgcgaacgt
    38221 atcacggagc tggaaagtta tcaattacct gcttgtaata gcgacatttt tcatggcgcg
    38281 ctaatgggcg cgaccatgtg attatccact aataaaaatt tgcttttgcg cgctgcgcga
    38341 gtgagttgtg agctaccggg agcataccct gcactccggc gtagccccca acatcgaatt
    38401 ggcctcggct cgcaccgtcc tcggcgcttt gaactgctcg ccggccagca aataaactgt
    38461 tcggtgctcg tgccgctggg gttcaggcgc ctggctggcg ctccacgcgg agctaggcac
    38521 catcggacgc cggccaagag tctttggctt tgtaggtcat aagctgagga gggaagttca
    38581 tgcgccaata acaaagggcc tcgcccacca ccaccaaccc ctcaaaattg cccttgcgcg
    38641 aagacttccc gcccgccagc ct
//
LOCUS       DAOPQG010000024        38195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1440_length_38195_cov_16.609046, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000024 DAOPQG010000000
VERSION     DAOPQG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 38195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38195
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1440_length_38195_cov_16.609046"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..2858)
                     /locus_tag="PKI07_04260"
     CDS             complement(30..2858)
                     /locus_tag="PKI07_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50298.1"
                     /translation="MKTPLPATRMAWLLALAMAPMTHCFGQYSQTTIATLASGTNAID
                     KSATALTATTFATAVAAAWNSGSGGVLHLPTAVPNDTTVYHGNYGNARRIVITSSANM
                     QNVAYNPTSFTPITAPNATTTKANQSDYTLTLGLIDTSTEQPPVDEVIKQAGLVVLSR
                     GTGAYPLDIRVTATFSDNSTEMATATVGRGSGTDDTFFGFIAPEGTGITSLRLQSFVT
                     NSVTPVSDRICWDDFAFITGPPLAVPLPVITDVTPPHGAVTLAANGLHFTATTYEAIP
                     TNQISLRVNAVDVSDQLVITGGGGEYQVSYAGLQPDQLYQIEIEVSNSSGATLRPHTL
                     HTYTSPTVLYDADGFDDDNIFPEGALQNVAHGHGVWEPNPDEPAFIVETGGAAGKVLQ
                     RDGTGARRADYLRFPAVSSGTLTADFDIWVTRADVRTIDIALQAEAGVMWGSFLAWGT
                     ITNELAYYDNTNWQPLGDWLADWHHLQVIHYLSGPAAGHYDVLVGGTPVGTQLPFRNA
                     PAGTAFNRFRIQSSDLTPWFEYGQMDNLVLTVGPETAVILPPTIADVSPADHAIAPAA
                     DGVQFRVLSQMPLTGPEVELRLNNVLTSLSFSGEGTNLIASHAPVAAGNYALDIRATN
                     TAGADQWTSQFIAADEAWMYDPSPGWTGPWSWTSGFPSLETAAPLDGAYLQYDIPGLG
                     VRNSIRQYTARPGLDLNQPHIIRWKFRLVENDFASNFNSFNDRVHFYARNAARPTGGT
                     DGSTSWSISATGDEQTPNSGVFAGQTFYLFDNVDGTGAVKLGNLVNSGIALLPGHVYA
                     VAVTVLPASGSYTVAIQDLTTSASFQSAAPHKFRAAGVTATSHTYLHFGVQAAPATDP
                     RALDLDSLEIIPDIQPVILLDPARTGTTFSFSFVSQDGMQHVVEYTSNLASPNWTTLT
                     TITGDGSLKQVMHPNSPDGPVFYRVVTQ"
     gene            complement(3957..4721)
                     /locus_tag="PKI07_04265"
     CDS             complement(3957..4721)
                     /locus_tag="PKI07_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50299.1"
                     /translation="MRPKQIPFNRSGDSSRGRLSAGRSAGRRPAFTLIELLVVIAIIA
                     ILAALLLPALARAKVKAQRISCLSNLHQIGLGLHMYASDHEDHLPTVFRTASIFTTYW
                     LRVNQEYKNLGLLFSGDYVKTPRAFYCVSGKLRADEVLAYDGPDNLWTNANVRSSYPC
                     RTVFKNNALATEWKLSACVTNVIYSDFIGVQNYRGGGIDQGYIYPVHNGEGYNRLFGD
                     GSGRWTRPGPLTRKVSAAVPSPVQMINYYQELDILR"
     gene            5074..6669
                     /locus_tag="PKI07_04270"
     CDS             5074..6669
                     /locus_tag="PKI07_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC50300.1"
                     /translation="MSAMVSASARPFLSLRDGVFRLGERLVFARTTWIWRWPEQWALV
                     GGNGSGKSLLADGLCGRLPLVGGELRYHFRPGPGLSPEEMIGQVSFERSKAGWGGAVA
                     QSRWNSLEEESSLRVRDFLAYERVMDINPFEVGAGSARARAQFERRQRGAVDLLALGP
                     LREQTLLSLSNGERQRVELARVLCRPLRLLILDEPFVGLDAATRRHFHAALERLMAGR
                     LRVLLVTTRPEDLPRRVTHLLQVENGQVVAAGRRAPGRAGAARGPERGAGFAPAVTAG
                     ARGVGARSAPPGSPPPVLVALRRVTVRHGRRVILRELDWTVRAGESWAVLGPNGAGKT
                     TLLGLVLGDHPQVYTNAVTVFGRRRGGGESQWDIKRHIGWVSPELHLHFKAAATCFEV
                     VASGFHDSIGLFEPVTRGQAAAARRWLRRFGLLGFAGTPLFGLSAGGQRMVLLARALV
                     KRPRLLALDEPCQGLDAAHRALFIGAVDELLRAGAVTALYITHRLDEIPPSIQRVLRL
                     DGKGRASEETLPPGGRLPGRVRA"
     gene            6748..7575
                     /gene="ccsA"
                     /locus_tag="PKI07_04275"
     CDS             6748..7575
                     /gene="ccsA"
                     /locus_tag="PKI07_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOC50301.1"
                     /translation="MDWLTDRHLFLLAVALYGLSLVYSVFLWRNGFRRDNRINYLLLL
                     AAFGPHSAAMLLRGFHLGRCPVTNLYEATLFIMWSIVGGYLGIGLWARLRFLGAFVSP
                     ALFALGVFALMPGLDTPPSVWPGLGTLWMSLHAALLSLAYGAFGLSAAAALMFLTQER
                     NLKVHKLRAISSLLPPIQRLAAAAERLLAAGFVFLTLGLGVGGLALARLEHPQAYRGD
                     PKIVWSVGVWLLYLGLIIWRWRFAQGGRRFALGVLGSFLFVLLTFWGTNVLSPLHNP"
     gene            7652..8710
                     /gene="hemA"
                     /locus_tag="PKI07_04280"
     CDS             7652..8710
                     /gene="hemA"
                     /locus_tag="PKI07_04280"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOC50302.1"
                     /translation="MPILVLGLSHHATPVAVRERFAFDAAGVPAALESLRGSGAADEA
                     VILSTCNRVEIYAATPREPSRALAGLREFLLASQDCPDAQIGGIYALGEPQSVQHLFR
                     VACGLDSMVLGETEVLGQLKQAYELARQHGHTGARLNRAFQRAFNVAKHIRTQTNIQR
                     GSVSVGSVAVELAEKVFSRLGDREVLVVGAGDTSEKTARALVSRGARGLAVANRSSDK
                     ARALAAELGGRAVSFADWLEELARVDIVISSTAAPHYLLDRARLAPLLPGRRNRPLLL
                     IDLAVPRDIEPEVNFLENVYLYNIDDLQAIADDYLKQRRQEVARCEQIIREKALALLE
                     DAGGGMWRGGVRVAFGHE"
     gene            8892..9971
                     /locus_tag="PKI07_04285"
     CDS             8892..9971
                     /locus_tag="PKI07_04285"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0018160 - peptidyl-pyrromethane cofactor
                     linkage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOC50303.1"
                     /translation="MPVSRPIILATRGSALALAQARRVLDQCRAAFPKLSFELKLIKT
                     TGDKLATAALAQAGKTLPKGLFTKELEVALLKHRADLAVHSLKDLPTELPPGLCLGAV
                     GKRADARDVLLYRDADYLRAAEADRQAAEWSPGQSARRGFPPGLTVAGLPSGATVATS
                     STRRKAQLLARNPALRLADLRGNVVTRLQRLAERPELDATVLALAGLARLNFRISPDG
                     WLQGDAVPDGLRATVLDLEEMLPCAGQGALGLEIRADDERLAAICERLNHFNTRQCVL
                     AERAFLAAMGGGCQSPVAAYAEVAGSCLHLRARALVDGQLRRAEARGPVAEALELGRQ
                     VAAALNPPPPEVGRPSEADGAGAFS"
     gene            10043..13048
                     /locus_tag="PKI07_04290"
     CDS             10043..13048
                     /locus_tag="PKI07_04290"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC50304.1"
                     /translation="MNAESTPNPERRFALDRLPWVVGAAALVVYLATLNRWVTFDSLA
                     LVSRVNGWDWLPALSQPLLLLLTSPFRLLPAPWIPLALNGFTAVCAALTLLTLARTVA
                     LLPHDRLPQQRELIQDPRALLMAPQAWVPIVLAALALGLQLTFWEHAISASGEMLDLL
                     MFAYVIRCLLEHRLDARPAWLDRAAFLFGVALANNWAMAGFLPIFGVALVRTKQFSFF
                     RLRTLQRLDQFRRRSFKPMLFADLRFLLRMMLLGLAGLSLFLLLPLVQALSPASGMGF
                     WTALKEAAMAYQFPLRLVYRVFFRFHREMALLLAVGSLLPVLLLSIRWGRLGGGLGRI
                     RFDPVMAILYLAHAFLLLLCLWIVCGPSCSPRQIAYHYNVRLPFLTFYYLTALSIGYY
                     SGFFLVFFGPVMRVRLTRRQNFRRVLYRGISLAVTALPGLLLAAMLLVNIPVLRASLA
                     PHLDRYVRLAAGSLPPEGAVVFSEDPIHLKLLCAAWGREGSANRYLPVDANSLPLQPY
                     RDWLSRKYPGRWPASPASAQLLPDYNVPLNALRQVQLLARVAQSNRLCCLPPSYGPLL
                     NHFYPQSQGLLHEMKPYPRDLLSPPPLTAEELASGQAFWQQAIPAAVKPVLQLVSTNE
                     LPRSGFSRWLLKMARLQTPPPVNARLLAGWYSRALNYWGVELQRNGQEQAATPCFELA
                     RDLNPDNVPARINLQCNHNLRARQPLTIADDTSLEEQFGKYRNIVQLLADNGPFDEPN
                     ACFKLGLGFAHTGLLRQSCQQFERVTQLVPDFLPARLLLADVCAEGLSPGWALQWVKE
                     IKANPAWQPLGLTNEVTLALTEAKVWFAVTNRPKAHGIIHALLATHPNNPFVYDRALA
                     AFMSQGSYAEALRVIDQRLQRDPLDANSLSDQGNLYVLMHQFSNAIPALTRALSLTNR
                     YATRRSLALAYLRTGQLEAAEAECNTLLKTFPDVYSTYSGLAEIALQRKDTNTAIHYY
                     ERHLAAAQARGDETRHVTEMLKLLQPGRR"
     gene            complement(13126..13914)
                     /locus_tag="PKI07_04295"
     CDS             complement(13126..13914)
                     /locus_tag="PKI07_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC50305.1"
                     /translation="MSPCSPLNDPEKTPGHWLLARMGKRVLRPGGRELTRRMLEQLDI
                     QPRDAVVEFAPGLGVTARMTLSRNPASYTAIERDEAAAAQVRSYLTGPNQRCHLGNAE
                     RTGLDDQSATVVYGEAMLTMQGAEQKRKIVAEAFRLLKPGGRYGIHELGFTPDDLPRD
                     IQEDISREMSRNIHVGARPLALREWRELLTGAGFQIQAEAVVPMHLLEPRRLIRDEGW
                     LSALRFVFNVLRRPQARKRVLSMRRMFRKHYRHLTAVSFVAVRP"
     gene            complement(14190..15386)
                     /locus_tag="PKI07_04300"
     CDS             complement(14190..15386)
                     /locus_tag="PKI07_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50306.1"
                     /translation="MRTLLVALGLLLASGYAYAAPAPADTNFFPIMAWNWAPKDPAVL
                     QTMREGGLTVAGFVNPDTLDACQAAGLKAIVSDARTSGYDWANVDEAKARTNIASLVA
                     EVGGHPAVYGYYLRDEPNAAMFPGLAKVADLIREMSPGKWPYINLFPDYASAEQLGAT
                     SYADYLERFIATCRPAIISYDNYSLMDDGSVRESYWSNLEAVRAACQRHGLEFWNIVL
                     AAAHFSYRELNGADFRFQVYTTLAYGGRGISYFTYFTPSTGNYRMGPIDQFGNKTPTW
                     YFMQHINLQVQKLAPTLLRLKSDAVYHFGPLPAGTSGPPAHSLLTGAGGAGVLAGEFT
                     HADGSRYLMVVNKDLAKSRICSLQFRAAPRQVSLVSPYSGALAPFRGEDVWLAPGGGV
                     LLKIEP"
     gene            complement(15746..16336)
                     /locus_tag="PKI07_04305"
     CDS             complement(15746..16336)
                     /locus_tag="PKI07_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50307.1"
                     /translation="MDNTRRYLRPPFEEALAAWKTLLTQHGRSTDLLWILEENLCFEK
                     DPGTTARVKLGFQTRFTPQPPDAARKTYFHFAENDARLVFYRLGANAGRSICLLLCDP
                     WFEPKTEADGYLRRDDWLISFFPGGDEQIEEVAEAERWHNRVVRGRPLSAVDFCMTLA
                     ALRELQAHGRVLTPDERFGLQILRSLRRARTPRGSG"
     gene            complement(16345..17298)
                     /locus_tag="PKI07_04310"
     CDS             complement(16345..17298)
                     /locus_tag="PKI07_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC50308.1"
                     /translation="MNFLVTGGAGFIGSHVCERLLRSGHAVWAFDDLNPFYDPRLKQR
                     NLDDLRALAGRFEFVHGDLTRRAALDDLLGRVRFDQVIHLAARAGVRPSLEEPALYQR
                     VNVEGTVNLLEAARVHGVRKLILASSSSVYGVNAKVPFAESDPIFRAISPYAASKLAC
                     EALGHVYHHVYGLEIVMLRLFTVYGPRQRPDLAIHKFARLIHSGQPVPVFGDGSTARD
                     YTYISDILDGILACTQRTFGYEIFNLGESQTVRLDQLVALLEDALGKRAILDRRPPQP
                     GDVPLTCADLTKARAGLGYHPRVQIEQGIPLFIDWFTRQPH"
     gene            17406..18299
                     /locus_tag="PKI07_04315"
     CDS             17406..18299
                     /locus_tag="PKI07_04315"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOC50309.1"
                     /translation="MATEPLLQLDLPGIPRLKSGKVREVFDLGDSLLFVATDRISAFD
                     CIMPNGIPRKGEVLTQLSHFWFAQTAAFQPHHLLARAEDPLPPRLQPYSAQLARRSMI
                     VKKARPLAIECVVRGYLAGSGWKEYRARQTVCGLRLPPGLTESSELPEPIFTPATKAE
                     SGHDLNISFAEAARLVGAGRAEQARAASLKLYAFARDYALRRGIIIADTKFEFGLVDD
                     QLVLIDEVLTPDSSRFWPADQYRPGAGQPSFDKQFVRDYLETLSWDKTPPAPPLPPEV
                     VARTQAKYLEAYERLTGRKLA"
     gene            18296..19216
                     /locus_tag="PKI07_04320"
     CDS             18296..19216
                     /locus_tag="PKI07_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOC50310.1"
                     /translation="MNQLVEDFLQYIRHERGQAEHTQKTYALLLGKFIAWANQRGLSD
                     WKSVQLAHLMAFLQHERERALAHQPPGSPRRLSSESLYLEIAALRAFYRFAENEKLLP
                     ANLAENLSLPRRWQRLPRALTGQEIERLLTPETPETPQSLCDQAMLELTYASGLRLAE
                     LRHLRLEQLHLAAGFITVIGKGNKERVVPVGRPAVAALERYLAAGRPQLVTPRTPANV
                     FLTRRGTPFAPVTLWLRIKRRARRAGIARNVTPHMLRHSFATHLLDHGADLRVIQELL
                     GHASISTTEVYTHVAATRLREVHRKYHPRS"
     gene            complement(20227..21432)
                     /locus_tag="PKI07_04325"
     CDS             complement(20227..21432)
                     /locus_tag="PKI07_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC50311.1"
                     /translation="MASFVYVARETASGREIRSSVEAATEQAAIMALLNRNMLVLSIQ
                     ERVGKQGRTSGGRVALADLVIFTRQLATMIDAGLAMVQSLQALAEQTTNKVMRDVIHD
                     VCARVEGGDSFSDALKKHPKAFSRLYVCMVAAGEKGGLLAEILARLATYLENTARLRK
                     KVKSATMYPAVVTVVAILITIFLLVKVVPVFGEIFRSFNATLPAPTMYLIMVSDIVKR
                     YLFLVLLAGGGAVYGWIYFIKTPTGRWIWDSRRIKLPVFGAIAHKICLARFARTLASL
                     VRSGVPILEVLQIVSQTVGNVVMEKAIKTTSTDIEQGESIATALAKHPVFPSMIVRMV
                     TAGEQTGKLDSMLERIADFLDEEIETTLSGLTALIEPILIVFLGVVIGGMVICMFLPI
                     FKLPEIVSR"
     gene            21853..23106
                     /locus_tag="PKI07_04330"
     CDS             21853..23106
                     /locus_tag="PKI07_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOC50312.1"
                     /translation="MKYNKWTVALAALGVVSLASAAKAEECLSPVKTALASTTISGYV
                     DTSAQWNFGTGNYNLPPYKFGGIDKADGFNLNVIQLRIEKPLDEQDWAAGYRADLWFG
                     PDANRLNTQSFWGEGNRAGTGDLAVRQAYAALRTPVGNGLDFKLGAFDSIIGYESIES
                     GNNPNYTRSYGHTIEPQTHTGLLASYRFCDCFSASAGVANTYGPAINERAFNGYYWSD
                     TRAESYKTYMGSVALTAPDSWGFLSGSTLYGGIVNGFGGETLTSYYAGLTVATPVEGL
                     RAGVAFDQINIHDVKNDNWAIAGYLSYQATEKLSLHARAEYYKDKVGNGGTLLFGRSF
                     ETEDGTVWVGPRKAEVLALTATVQYDLWKNVISRLELRWDHSLDGHLFGGKSPNSYDA
                     DTDTYTSSANLKNAWMLAANVIYKF"
     gene            complement(23237..23539)
                     /locus_tag="PKI07_04335"
     CDS             complement(23237..23539)
                     /locus_tag="PKI07_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50313.1"
                     /translation="MALTRHCWKCGTEYTLRGTPGRSECCERCRSDLRVCRNCISYDP
                     RVAEQCRDRRAELVAEKHLANYCEYFEFARREYVPPAEDTSREAKARDTLRKLLGD"
     gene            complement(23610..25094)
                     /locus_tag="PKI07_04340"
     CDS             complement(23610..25094)
                     /locus_tag="PKI07_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOC50314.1"
                     /translation="MKIRFWGNWRCVNRVLIPGLAVAAAFSSYGAAVSSSPNANQRRP
                     SIIFILADDLGYGDLGCYGQTKIQTPNLDRLAAEGMRFTQFYAGSTVCAPSRGALMTG
                     LHTGHARVRGNAAVALRPQDLTVAEILQRAGYRTGLIGKWGLGNEHTTGVPQKKGFDE
                     FLGFLDQVHAHNYYPDYLWRYDPPGPAKPGYDGRRDFLENQGGRRGLYVHDQFTTAAL
                     NFIQINQPDRFNHYRPFFLYLAYTIPHANNEEGRRTGNGMQVPSDAPYTDQSWPPPEK
                     NKAAMITRMDADIGRILDKLQELKLADNTVVFFASDNGPHREGGVDPQFFQSSGPLRG
                     LKRDLYEGGIRVPFIVRWPARIKPGARNDQPWAFWDFLPTAAAIAQAPVPEPIDGRSL
                     LPTLLGQPQTNPHEFFYWEFHERGFQQAVRYGDWKAVRLRAGAPLELYHLKTDPGEKE
                     NVADRNPQVVARIEDYLKTARTESEHWPLKDADAPTRAPEPKTK"
     gene            25404..28217
                     /gene="ileS"
                     /locus_tag="PKI07_04345"
     CDS             25404..28217
                     /gene="ileS"
                     /locus_tag="PKI07_04345"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOC50315.1"
                     /translation="MDYKNTLNLPQTDFPMKADLIKREPERLRKWDAAGLYAAIQARR
                     AGAEKFVLHDGPPFANGEVHIGTALNKILKDIIIKYKTLRGFHAPYVPGWDCHGLPIE
                     FKVMQDMRKAGNSATDAASIRQACETYARKYIDLQRAQFQRLGVLGDWANPYLTFAKE
                     YEADELRLFAGLVEQGMVYRGKKPVYWSIPCRTALAEAEVEYKDHVSQSVYVKFPLVG
                     HPGTSVVIWTTTPWTLPANLAVAYNSTFHYSLVLVGREQYIVSALLLPAIAAKCGWQD
                     YQIVRSLEGGHLAQMEYQHPFCRRTGKFFKGDSFVDNTTGTGFVHIAPGHGLEDYHLG
                     IEQGLPVYSPVDDDGKFVHTSDLPAEQQMPAEMLGRSVGEKHGKSEANEAVLHELRAR
                     QALLHQEDYTHSYPHCWRSKTPIIFRAMAQWFIKIDHAGFRDRALAEIQRVHWVPEWG
                     RSRIEAAVKGRPDWCISRQRTWGVPIPAFYDASGNPILDPRIVRNAADLFEQHGSNVW
                     FERSAAELWAALQPADWTGPASVAKSNDTLDVWIDSGSSSRAVIARRPEIRGAGHPFQ
                     CDMYLEGSDQHRGWFQSSLLLSLAGNGAAPYRTVLTHGFMVDADREKISKSQQGQGGY
                     EKPQTAEAYVKKWGADIIRLWVASQDFRNDIIVSEERIQTVAETYRVLRNALRYELAN
                     LADFDPAAQAVPDGQLTGLDRWILDEFSQLERDVLAAYDRHEFHVVYQRVSQFVAVEL
                     SALYHDVVKDRLYTDPANAPRRRSTQTALYRLVTGLCRLLSPILAFTADEAWEFVPGR
                     TVPSVHLLTWEPLGFERPEDERAAWRTLLELRERALPELEKARQAKSIGKALEAKLTF
                     AGAGPELATAQAHRESLRELLNVSQLEIQPTAEAALTISLARAAGEKCGRCWHWETHV
                     GVDAGHPTLCARCAQAVRESVPA"
     gene            28461..28733
                     /locus_tag="PKI07_04350"
     CDS             28461..28733
                     /locus_tag="PKI07_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HOC50316.1"
                     /translation="MPQAIMDPEQVRRFAEELKRFNHDLKDRMVSLQARFAALGDTWQ
                     DQEHAKFAEEFKHTMKALRQFVEVSNQHTPYLLRKAQRIEEYLNQR"
     gene            28737..29273
                     /locus_tag="PKI07_04355"
     CDS             28737..29273
                     /locus_tag="PKI07_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50317.1"
                     /translation="MAERAQVTSSDALELFRSNLLLYLDKARPALDEVSAEVMRMRNW
                     LEDEQRVYWESQVRRRRREFDEAQQALFSAKLSLLDRASSAEQMMAHRARRALQAAEE
                     KLRKVKQWDRVFESRTAPLVKQMEKLHTVLAHDMVQAVAFLTRTLETLEAYRRVAPPA
                     SAGATRASGETPSPGGAP"
     gene            29270..29476
                     /locus_tag="PKI07_04360"
     CDS             29270..29476
                     /locus_tag="PKI07_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50318.1"
                     /translation="MKGSGARLGGLTKELRAQWLDTKSYWRDARGEEFERRYMEELFA
                     SVDRAATVMEQLDKLLTQIRRDCE"
     gene            29469..33497
                     /locus_tag="PKI07_04365"
     CDS             29469..33497
                     /locus_tag="PKI07_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HOC50319.1"
                     /translation="MSSNLGVGQTLELLERLQATVRAFAARSEKLNAEYHAQRGREQR
                     LREAVRERQAEELAAADREAEAARAAASQALAARHAARKARIGAAYQASKEQRLAEIE
                     ARTGARKYELQKAMLQAERDRDAGQAAFGTAFEEFRHLLAAEQAELAARETEARKAFQ
                     GYRKLVRRLAAADRKTPAPARDEQQLLAELRECLAQTRDDLRRFRRFLPLRWFKSLPL
                     WGLIALGLVPLLLRQFGSGPAAYGKAGLGAVGGLVVVLIIRSLAQRRVRRQAAAIADG
                     LAQARCCHDAAAERAEAHYQQELERLGSEFANTTQTVDQELKRVLAEASELRVACRTA
                     SDEKAARALARNDHLQRIRQERQHRQQVEAGEQRRRTVVARGQAETRASEQREAALNA
                     DYQAAGQALTAEWQRAIGPLYADIQSLNALADKLFPPWQAPGFEAWTPPAQFAGAAKF
                     GRLEVEMEKLAPAALRDGRLAWPGPARFSLPACLAYPGQGSILFETAEQGRDEAIGAL
                     NNLLLRLLATAPPGRLNFTIIDPVGLGQNFAGIMHLADHEAQLINSRIWTQPGQIEQK
                     LADLNEHMEKVIQMYLRNEYATIAEYNQQAGVIAEKYYFLVVADFPANFTETAAKRLL
                     SIAASGARCGVYTLIHWDRRLTLPPEFIPEELRADSVGLSAVPDGRLSPRAQAAAIPG
                     DNRARGDTRRSEPAGGGGAVFRLAGPARPGIRLELEPPPPPEFAIEFVNRVGVSSRDS
                     SRVEVPFAHVAPPAAEIWSQDTAKELLVPIGRTGATKFQYLALGKGTCQHALIAGKTG
                     SGKSTLFHVIITNLALWCSPEQVEFYLVDFKKGVEFKCYATHRLPHARVVAIESDREF
                     GLSVLQRVDDELKRRGDLFRQRGVQDIAGYLRTGGPEPIPRSLLIIDEFQEFFVEDDR
                     ISQTASLLLDRLVRQGRAFGIHVLLGSQTLGGAYTVARTTLGQMVIRIALQCNEADAY
                     LIMDENNPAPRLLSRPGEGIYNDMAGRLEGNSPFQVVWLPDQVRETALAEVRVRADQA
                     GAQRPGADGGPLYPGPIVFEGNAPADVRENAPLRRLLEAPAIAPAAAGRIWLGAPNSI
                     KGPTEAVFPRQSGSHLLLVGQRDETLLGLLSLGLLALAGQYPAGAARFILCDGTAPGT
                     APRQYMEQIIRAIPHPVTQARAGDLDEIFKGLAADMRQRAEAVDPEAAPPVFLFLHGL
                     QKYGKLRYEEDFSFATGDAEAEANPAQRLNHLICEGARLGCHVIATCDTYNNVTRFLS
                     RKALSEFELRVLFQMSANDSASLIDSPKASLLGLHRALFFNAQEGTLETFRPYAPPAR
                     EWLEQTARNLARLLAPGNRAPA"
     gene            33602..34045
                     /locus_tag="PKI07_04370"
     CDS             33602..34045
                     /locus_tag="PKI07_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HOC50320.1"
                     /translation="MRNTFTLLASLAMAGALLTGCANTEKKLGRGMSNMTELVRLGEM
                     RRSIEQTALFDQPGGHYATGFLRGLNRTLARTGVGLYEVVTAPFPPYDPIFTDYLSPN
                     PVYPDNYRPGILDDAMFATDANMGFSGGDIFPYIPGSRFKIFETH"
     gene            34163..35041
                     /gene="ispE"
                     /locus_tag="PKI07_04375"
     CDS             34163..35041
                     /gene="ispE"
                     /locus_tag="PKI07_04375"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOC50321.1"
                     /translation="MGVALEIQSPGKVNLLLNILGRRPDGFHELETVFHPVQVFDHLR
                     LARGGSGIQLRCSDPRLPTDARNLVYRAAAMFLKAAAIPDGVRLDLRKNLPLAAGLGG
                     GSSNAAITLLGLNELFGEPLTPEHLHRLAAALGSDVSFFLRRQPALAGGRGERLEPLP
                     PFPALRGAAFVLIHPGFGISTAWAYQQLARFPDALNGRPGRARQLVALLQTADLPTAG
                     AAFYNALEAPALAKYPLLALFQEFLRAHGAAAALMSGSGSTTFAVVSGLAAARALAEK
                     FKARFGASNWIAVLPV"
     gene            35187..36014
                     /locus_tag="PKI07_04380"
     CDS             35187..36014
                     /locus_tag="PKI07_04380"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC50322.1"
                     /translation="MITRHRFLSGAGWIRLLFTVLPALGLAACARAPRADAPQTFRVM
                     TYNIHHGAGLDERIDFARIADLIRREQADIVALQEVDKGVQRTARRDCPAELAALTGL
                     TCVFSNNFHFQGGEYGNAVLTRFPVKRWTNRHYQMLRPGEQRGLLQVVLDVRGRELVF
                     LNTHIDYRPGDDERLAHAGEILETARAWPGRPVILCGDFNDTPGSRTHQKIATAFLDT
                     WLTAGTGDGNTFPAQKPATRIDYVWLSRGSALDPVKAWVPESDASDHLPVVVEFRWR"
     gene            complement(36022..37494)
                     /locus_tag="PKI07_04385"
     CDS             complement(36022..37494)
                     /locus_tag="PKI07_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOC50323.1"
                     /translation="MPPQMSKYYVACDLGAESGRVMLGTLHEDSLTISEVRSFPNTPI
                     LEKDAWLWNIPWLYEEILQGLNAVGTYEEAVDSISCDSWGGDYLLFEPDGSLITPTYH
                     YRDRRGEEGMRKVGAAIPPEALYAETGGQLSPGSTLSQLAAEKPRRLARAERLLPVAD
                     AFNYLLAGVARGERSLAGATRLYNPATRAWSPRLLQAAGVPARLLPPLVAAGTELGPL
                     QPAIAKSTHLEDTVVVASCSDQTAAALTGLPVPEGESWAYLRLGSRTDVGALIGQPIL
                     TEAARAQGFTHEPGYGDSIRFFKQTMGLWLLEECRRFWKAQDREIDDALLTHLAGASP
                     AFEALIDPADPRFAAAGDMPLKVQAFCRETRQPVPRKPGAIIRCVLESLALSYRQALE
                     ELEALAGRPVARLYLLGGAGGDLLQHFIANAVRRPVVAAPPDAAALGNVVAQALALGH
                     LTSLEQARELLRHSTKTSPLQPYATAWDAAFARLRELRAA"
     gene            37729..37926
                     /locus_tag="PKI07_04390"
     CDS             37729..37926
                     /locus_tag="PKI07_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50324.1"
                     /translation="MMTRRVSPGLSALPWHDEEQHPVERPRQFGTLGDALSGHDPVLT
                     RSLAFDKTFAQPKALQTPDRP"
BASE COUNT         6393 a        12450 c        12675 g         6677 t
ORIGIN      
        1 ctggccgccg ccgggcacgc agattcagct tattgagtga cgacccggta aaacaccggc
       61 ccgtccggcg aattcggatg catcacctgt ttgagcgacc catcgccggt aatcgtcgtc
      121 agcgtggtcc aatttggcga cgccaggttg ctggtgtatt ccaccacatg ctgcattcca
      181 tcctgcgaga cgaaggagaa ggagaacgtc gtgcccgttc gcgccgggtc cagcaggatc
      241 accggttgaa tgtccgggat gatttcgagc gaatccaggt cgagcgcgcg cgggtcggtg
      301 gccggcgcag cctgcacccc aaaatgcagg taggtatggc tggtcgccgt gacccccgcc
      361 gcccggaact tatgcggggc ggcgctttgg aaggaggcgg aggtggtcag gtcctggatc
      421 gcaaccgtat aggagccgct ggcgggcaga accgtcaccg ccaccgcgta cacatgcccc
      481 ggaaggagcg caatcccgct gttgaccagg ttgcccaact tgaccgcgcc cgtgccgtca
      541 acattatcga acaggtagaa ggtctggccg gcgaagactc ccgaattggg cgtctgctca
      601 tcgcccgtgg cgctgatgga ccaactggtg ctgccgtcgg ttccgccggt gggccgcgcc
      661 gcgttgcggg cgtagaaatg cacgcggtcg ttgaagctat tgaagttgga cgcaaagtcg
      721 ttttccacca gccggaattt ccaacggatg atgtgcggct ggttcaaatc caggcccggg
      781 cgcgcggtgt attggcgaat cgaattgcgc accccaaggc cggggatgtc atactggaga
      841 tacgccccat ccagcggagc ggccgtttcc aggctcggga agccgctggt ccagctccag
      901 gggccggtcc agcccgggct cggatcgtac atccaggctt catccgcggc gatgaattgg
      961 gaagtccatt gatccgcccc cgccgtattg gtggcccgga tgtccagggc gtaattgccg
     1021 gccgccaccg gcgcgtggga ggcgatcaaa ttggtgccct cgccggagaa actcaggctg
     1081 gtcagcacat tgttgaggcg cagctccacc tccggcccgg tcagcggcat ctgcgaaagc
     1141 acccggaact gcaccccgtc ggcggccggg gcgatggcgt ggtcagcggg gctcacatcc
     1201 gcaatggttg gcggcagaat gaccgccgtc tccgggccca cggtaagaac caggttatcc
     1261 atctggccat actcgaacca cggcgtcagg tcggaggact gaatgcggaa gcggttaaag
     1321 gccgtgcccg ccggcgcatt gcggaaagga agctgggtgc cgaccggcgt gccgcccacc
     1381 agcacatcat aatggcccgc cgccgggccg ctgaggtaat gaatcacctg gagatgatgc
     1441 cagtcggcga gccagtcgcc gagcggctgc caattggtat tgtcgtagta ggccagttcg
     1501 ttggtgatcg tgccccaggc caggaaactg ccccacatca cgccggcttc cgcctgcagg
     1561 gcaatatcaa tggtccgcac gtccgcgcgg gtcacccaga tatcaaagtc cgcggtcagc
     1621 gtgccggagg acaccgccgg aaaccggagg tagtccgccc gccttgcgcc cgtgccgtcc
     1681 cgctgcagca ccttcccggc cgcgccgccc gtctccacga tgaacgccgg ctcgtccggg
     1741 ttcggctccc acacgccgtg gccatgcgcc acgttttgca acgccccctc cgggaagata
     1801 ttatcgtcgt cgaatccgtc cgcgtcgtag agcactgtgg ggctggtata ggtgtgcaag
     1861 gtgtgcggcc gcagggtggc tccgctcgag ttggacacct caatttcgat ctggtaaagc
     1921 tggtcgggct gcaatcccgc ataggacacc tggtactccc cgccgccgcc cgtgatcacg
     1981 agctggtccg agacgtccac cgcgttgacc cggagcgaaa tctgattggt cgggatcgcc
     2041 tcgtacgtgg tcgcggtaaa atggaggcca ttggcggcaa gcgtcaccgc gccgtgcggc
     2101 ggcgtcacat ccgtaatcac cggcagcggc acggccagcg gcgggccggt aatgaacgca
     2161 aagtcgtccc agcaaatccg gtcggatacc ggcgtcaccg agttggtcac gaacgattgc
     2221 aaacgcaggc tcgtgatgcc ggtgccctcg ggggcgataa agccgaagaa ggtatcatcc
     2281 gtgccggagc cgcgcccgac cgtggccgtc gccatctcag tggagttatc gctgaacgtc
     2341 gccgtcaccc ggatatccag cggatacgcg ccggtgcccc gggacaatac caccagcccg
     2401 gcctgcttga tgacttcgtc caccggcggc tgctcggtgg aggtgtcaat aagcccgagg
     2461 gtaagggtgt agtcgctctg gttggctttg gtggtggtcg cattgggcgc cgtaatgggg
     2521 gtaaacgagg tgggattata ggccacgttt tgcatgttgg cgctgctggt gatcacaatg
     2581 cgccgggcat tcccataatt gccatggtaa acagtcgtgt cgttcggaac cgccgtcggc
     2641 agatgcagaa ccccgcctga gccactattc caggcggccg ccacggcggt cgcaaaggtg
     2701 gtcgcggtaa gcgcggtggc actcttgtca atggcgttgg tgccggatgc cagagtggca
     2761 atagttgtct gagagtactg cccgaagcag tgagtcatcg gcgccatagc cagggccagc
     2821 agccaggcca tccgagttgc agggagagga gtcttcatgg gttttagttt tactttttac
     2881 gccatggccc aggcagcccc acaatggcgc cacaccactg ggccacatgc gcgagcatat
     2941 tatccgccca gatatgtcag cgcaactcta aaatatcgga atagaaatcg cttttcgccg
     3001 gaacgattcc gctttcaatt tccactcgac cccacagaac gcgggggtcc ctggccttgc
     3061 ggacgaaaga cattcgcaag cccgcgttcc gcccgctgaa tccgccagcc aaccggttcg
     3121 ttgcggctat ccgctggccg gttaggcccg ttttggctcc agcggaacga gggcgcacgg
     3181 ttggacgatg ccacggcaac gtttccgata aggcatttgc gaagccaaaa gccatccggg
     3241 cgcccgcttg cccttgcaaa tccccctccc gccagcctca gattcataac tagctgtttt
     3301 tactggactt attacccgcc acaccgcccc ttctcatcat ttcggccttt cccggcatcc
     3361 acccacatta tccagatgcc gtggaaatac cctgccttca ggccgtgtac aaggggtgta
     3421 caagggatgc ccatcgggtg taccagctaa aatcttatgg actcccgatg agcatccctt
     3481 gtacacccct tgtacacggc ccggaggcag ggggatttca ggggatgtcc agggtaagcc
     3541 gaggccggcg caatgcagat tttgcctctg ttttacccct ctcgacggct aaacccttga
     3601 ttttacccgc gaaaggcccg ggattcagcc gccatgcctt tggattggct aatcaaaggg
     3661 ttcgccgggg ccaaggtttg ggcggtccgg cccgcccgga ttccgaggga acgagggcgg
     3721 gcggttgaac ggcccaactg agtcgttctg gttaagccgg ccgcgacctg ggagccaggg
     3781 gatatgcgtc gcatttccct gagcggggca agcgtcggaa acctgccgga atggcatccc
     3841 ggcgaaacga gcgatcccgg gcctgtcaac agttgcccgt cctttccggt gagcgtcgct
     3901 gccgccggag ccgttacgtt ttcacggccg gaggcccagc ccccggggat cgttcatcac
     3961 ctgaggatgt ccaactcctg gtagtagtta atcatctgca cggggctggg aaccgcggca
     4021 gaaactttcc gggtcagcgg ccccgggcgc gtccatcggc cggagccgtc gccgaacaac
     4081 cggttgtagc cctcgccgtt gtggaccggg tagatgtagc cctggtcaat gccgccgccc
     4141 cggtagtttt ggacgccgat gaagtcgctg taaatcacgt tggtgacgca agccgacagt
     4201 ttccactcgg tcgccaaagc gttgtttttg aacacggtgc ggcagggata ggaagagcgc
     4261 acattcgcat tggtccagag attgtccggc ccgtcatacg ccagcacttc atccgcgcgc
     4321 agcttgccgg atacgcaata aaaggccctg ggggtcttga cgtaatcgcc actgaaaagc
     4381 agccccaggt tcttatactc ctggttgacg cgcagccagt aggtggtgaa aatcgaggcg
     4441 gtgcggaaaa ccgtcggcag gtgatcctcg tgatccgacg catacatatg cagccccaac
     4501 ccaatctgat gcaggttgct caggcaactg atccgctgcg cctttacttt cgcccgggcc
     4561 agcgccggca gcagcagcgc cgctaaaatg gcgatgatgg caatcaccac cagcagctca
     4621 atcaaggtga acgccggccg ccgcccggcg gaccggccgg cggaaaggcg cccgcgcgag
     4681 ctatcgccgc tccggttaaa ggggatttgt ttgggacgca cccgcaggcc ttgaatcctg
     4741 cgtgggtgaa gacagacggg ctttcgagtc tcaccaagca tggcgcgagt gtaataaaac
     4801 ccaactccgg ccgcaacagt tttttgttgc aatgtttgcg ccccggaacc cggccgcgac
     4861 gggatggccc ggaaatcccg cgcggcgcgg gcaagcccgt ggggcgccgc cggggcgaaa
     4921 accgggagtg acggcacccc ctcgcaggag cggggatttt ggcgggcggg cgcgggcaag
     4981 gagagccgtc cgcgcccggg cgcgctttcc cggcgcggta aaatagggcg tgcattgccg
     5041 gctggttccg gcgcagattt cggatggttg tagatgagtg caatggtttc ggcttccgcg
     5101 cggccgtttc tctccctgcg ggatggggtc ttccggctgg gggagcggct ggtctttgcg
     5161 cgcaccacct ggatttggcg gtggcccgag cagtgggcgc tggtcggggg caacggctcg
     5221 ggcaagtcac tgctggcgga tggcctgtgc gggcggctgc cgctggtcgg gggcgagttg
     5281 cgctaccatt tccggcccgg gccgggcttg agcccggagg aaatgattgg ccaggtttcg
     5341 ttcgagcgca gcaaggccgg ctggggcggc gcggtggcgc agtcccgctg gaacagcctg
     5401 gaggaggaga gctccctgcg ggtgcgcgat tttctcgctt atgaacgggt catggatatc
     5461 aacccgtttg aagtgggcgc gggttcggcg cgggcgcggg cgcaattcga gcgccgccag
     5521 cggggggcgg tggatttgct ggcgcttggc ccgttgcggg agcagacgct gctttccctg
     5581 tccaacgggg agcggcagcg ggtcgagctg gcgcgggtgc tgtgccgtcc gctgcggctg
     5641 ctgattctgg acgagccgtt tgtgggcctg gatgccgcca cccggaggca tttccacgcg
     5701 gccctggaac ggctgatggc cggacggttg cgggtgctgc tggtcaccac gcggccggag
     5761 gatttgcccc ggcgcgtgac ccacttgctc caggtggaga acggccaggt ggtggcggcg
     5821 ggccggcgcg cgccgggccg ggccggggcc gcccggggcc cggagcgcgg cgccgggttt
     5881 gcgcccgccg tgacggccgg ggcgcgcggg gttggcgcgc gttccgcgcc gccgggatcg
     5941 ccgccgcccg tcctggtggc gctgcgccgg gtcaccgtcc gccacgggcg ccgggttatt
     6001 ttgcgcgagc tcgattggac ggtgcgggcg ggggaaagct gggcggtgct ggggcccaac
     6061 ggcgccggca agaccacgct gctggggctg gtgctgggcg atcatccgca ggtctatacc
     6121 aacgcggtga cggtgtttgg gcggcgccgg ggcggcggcg agtcgcagtg ggatatcaag
     6181 cgccacattg gctgggtttc gcccgagctg catctgcatt tcaaggccgc ggctacgtgt
     6241 ttcgaggtgg tggcgtcggg gtttcacgat tccattggcc tgttcgagcc ggtcacccgg
     6301 ggccaggccg ccgccgcgcg gcgctggctg cgccggtttg ggttgctggg ctttgccggg
     6361 acgccgctgt tcggattgtc ggcgggcggg cagcggatgg ttttgctggc gcgggcgctg
     6421 gtcaagcggc cgcgcttgct ggcgctggac gaaccgtgcc aggggctgga cgcggcgcac
     6481 cgcgcgctgt tcatcggcgc cgtggacgag ctgctgcggg ccggggcggt gaccgccctt
     6541 tatattactc atcggctgga tgaaatcccg ccctcgattc aacgggtgct gcgtttggac
     6601 gggaaggggc gggcgagcga ggagacgttg ccgcccggag gccgccttcc ggggcgcgtc
     6661 cgggcctgac ccgctcccgc gccggggatc gtccggcggg cggcaattgc cgcttgcgcc
     6721 gggtttgggg cgggttaacc ttgcggcatg gattggttga cggaccgaca cctgttcctg
     6781 ctggcggtgg ccctttacgg gctgagcctg gtgtattcgg tgtttttgtg gcgcaatggg
     6841 ttccgccgcg ataatcgcat taattatctg ctgttgctgg cggcctttgg cccgcacagc
     6901 gcggcgatgc tgctgcgcgg gtttcacctg ggccgctgcc ccgtcaccaa cctttacgag
     6961 gccacgctgt tcatcatgtg gagcatcgtg gggggctatt tggggatcgg gctgtgggcg
     7021 cggttgcggt ttctgggcgc gtttgtgtcg ccggcgcttt ttgcgctggg ggtgtttgcc
     7081 ctgatgcccg ggctggacac cccgccgagc gtctggcccg ggctggggac tctctggatg
     7141 agcctgcacg ccgcgctgct ttcgctggcc tacggcgcgt tcggcctcag cgcggcggcg
     7201 gccctgatgt ttctcaccca ggaacgcaac ttgaaggtcc acaagctgcg ggcgatcagt
     7261 tcgctcctgc cgccgatcca gcggctggcg gcggcggcgg aacggctgct ggctgcggga
     7321 tttgttttcc tgacgctggg gctgggggtc ggcgggctgg cgctggcgcg cctggagcat
     7381 ccccaggcgt accgcgggga tccgaagatt gtctggtcgg tgggggtctg gctgctttac
     7441 ctgggcctga ttatttggcg ctggcgcttt gcccagggcg ggcgccgctt cgcgctgggg
     7501 gtcctgggca gcttcctctt tgtgctgctg accttctggg gcaccaatgt cctgtcgccg
     7561 ctgcataacc catgatggcc actttccgca tccgccccag ggcgtcgtcc gcggccggag
     7621 ccggcgcggc gggtgtgcct gattgcttcc catgccgatt ctcgtccttg gcctgagtca
     7681 tcatgccacg ccggtggcgg tgcgggagcg ctttgcgttt gacgcggcgg gcgtgccggc
     7741 ggcgctggag tcgctgcgag gctcgggcgc cgctgatgag gcggtcatcc tgtccacctg
     7801 caaccgcgtt gagatctacg ccgccacgcc gcgggagccg tcccgcgccc tggccgggct
     7861 gcgggaattc ctgctggcgt cccaggattg ccccgacgcg cagatcgggg ggatttacgc
     7921 gctgggcgag ccgcaaagcg tccagcatct tttccgcgtg gcctgcgggc tggattcgat
     7981 ggtgctcggg gaaacggagg ttctgggcca gcttaaacag gcgtatgagc tggcccgcca
     8041 gcatggccat accggcgccc ggctcaaccg ggcctttcag cgcgccttca acgtcgccaa
     8101 acacatccgc acccaaacca acatccagcg cggcagcgtc tccgtcgggt cggtcgccgt
     8161 ggagctggcg gaaaaggttt tcagccgcct tggcgaccgc gaggtgctgg tcgtcggcgc
     8221 gggcgacacg agcgaaaaga ctgcgcgcgc gctggtttcg cgcggcgccc gcggcctggc
     8281 ggtggccaat cgttcgtccg acaaggcgcg ggcgctggcc gcggaactgg ggggccgcgc
     8341 ggtgtccttc gcggactggc tggaggagct cgcgcgcgtg gatattgtca tcagcagcac
     8401 cgcggcgccg cattacctgc tcgatcgcgc ccggctggcg ccgctgctgc cggggcgccg
     8461 caaccgtccc ctgctgctca ttgacctggc cgtgccccgg gacattgagc cggaagtcaa
     8521 tttcctggag aatgtttacc tctacaacat tgatgatctg caggccatcg ccgacgatta
     8581 cctcaaacag cgccggcagg aagtggcccg ctgcgagcag atcatccgcg aaaaggcgct
     8641 ggcgctgctg gaagacgcgg ggggcggaat gtggcgcggc ggggtgcggg tggcgtttgg
     8701 gcatgagtga cggggccggg gcgggaaaga gttttatgac cttgatggaa tggctggcaa
     8761 cgaggccgcg ggcgcggaaa gacgctgcgc tttggcgggg gggggcgtat cggcccggcg
     8821 gcccgtcggc ccggcatcgt tggcggcggc gcccgcggag ccttcgccgt ctctccgccc
     8881 tctgacccgc catgcccgtc tcccgcccca tcatcctggc cacccgcggc agcgccctgg
     8941 cgctggccca ggcccgccgg gtgctggacc agtgccgcgc cgcctttccg aagctgagtt
     9001 tcgagttgaa gctcatcaag accacgggcg acaagctcgc gacggctgcc ctggcgcagg
     9061 ccggcaaaac cctgcccaag ggactcttta ccaaggagct tgaagtggcc ctgctcaagc
     9121 atcgcgccga cctggcggtg cacagcctga aagacctgcc cacggaactg ccgccggggc
     9181 tttgcctggg cgccgttggc aagcgcgcgg atgcgcgcga tgtgctgctc taccgcgatg
     9241 ccgattacct gcgcgccgcc gaagccgacc ggcaggcggc cgaatggtcg cccggccaat
     9301 ccgcccgccg cgggttcccg cccgggctga ccgtggcggg gctgccgtcc ggcgcgacgg
     9361 tggccaccag cagcacccgg cgcaaagccc agttgctcgc gcggaatccc gccctccgcc
     9421 ttgcggacct ccgtggcaac gtggtcaccc gcctgcaaag gctggccgaa cggccggagc
     9481 tggacgcgac cgtgctggcg ctggccggcc tggcccggct taacttccgc atcagcccgg
     9541 atggctggct ccagggcgac gcggtgccgg acggcttgcg ggcgacggtc cttgacctgg
     9601 aggagatgct gccttgcgcc ggccagggcg cgctgggcct tgagattcgc gcggacgacg
     9661 agcgcctggc cgctatctgc gagcggctca accatttcaa cacgcgccaa tgcgtcctgg
     9721 ccgagcgcgc cttcctggcc gccatgggcg gcggctgcca aagccccgtc gccgcctacg
     9781 ccgaagtcgc gggcagctgc ctccacctgc gcgcccgcgc gctggtggac ggccagctcc
     9841 gccgggcgga ggcccgcggg cccgtggcgg aagcgctcga gctggggcgg caggtggccg
     9901 cggcgctcaa cccgccgccg cccgaggttg gccggccaag cgaggcggac ggagcgggcg
     9961 cttttagttg aacccggcgg gggaaacata ttaagtggcc ttggccgccg ttgtgtggtt
    10021 caataaggcc gcttgcgaaa gcatgaatgc tgaatccaca cccaatcccg agcgccgttt
    10081 tgcgctcgac cgcctgccgt gggtggtcgg cgcggccgcc ctggtggttt acctggcgac
    10141 gctgaaccgc tgggtgacct tcgacagcct tgcgctggtg agccgggtca acggctggga
    10201 ctggctgccc gcgctctccc agccgctgct gctcttgctg accagcccgt tccgcttgct
    10261 gccggccccc tggattcccc tggcgctgaa tgggttcacc gctgtctgcg ccgcgctgac
    10321 cctgctgacc ctggcgcgca ccgtggcgct gctgccgcat gaccggctgc cgcaacagcg
    10381 cgagctgatc caggacccgc gcgcgctgct catggcgccc caggcctggg ttccgattgt
    10441 cctggcggcc ctcgcgctgg ggctgcaatt aacgttctgg gaacacgcca tcagcgcttc
    10501 gggcgaaatg ctggacctgc tgatgttcgc ctatgtcatc cgctgtctgc tggagcaccg
    10561 gctcgacgcg cggccggcgt ggctggaccg ggcggcgttt ctttttggcg tcgccctggc
    10621 caacaactgg gccatggccg gcttcctgcc gatcttcggg gtggcgcttg tccggaccaa
    10681 acaattcagc ttcttccgcc tgcgcacgct tcagcgcctg gatcaattcc ggcggcgcag
    10741 cttcaaacct atgctattcg ccgatctgcg ctttctgctg cgcatgatgc tgctcgggct
    10801 ggcgggcctg tcgctcttcc tcttgctgcc gctggtgcag gcgctctccc cggcttcggg
    10861 gatgggtttc tggacggcgc tgaaagaggc ggcgatggct tatcaattcc cgctccgcct
    10921 ggtctatcgc gtgttcttcc gtttccaccg cgagatggcg ctgctgctgg cggttggctc
    10981 gctgctgccg gttctgctgc tgagcatccg ctggggccga ttaggcggcg gcctgggccg
    11041 catccggttc gacccggtca tggccatcct gtatctggcg cacgccttcc ttctgctgct
    11101 ctgcttgtgg attgtctgcg ggccgtcctg cagcccccgc cagattgcct atcattataa
    11161 cgtgcggctg cccttcctga cgttctatta tctcaccgcc ctgagcatcg gctattacag
    11221 cggtttcttc ctcgtgtttt tcggccccgt catgcgcgtg cgcctcaccc ggcggcagaa
    11281 ctttcggcgc gtcctctacc ggggaatctc cctggccgtc acggcgctgc ccggtttgct
    11341 gctggccgcg atgcttttgg taaacattcc cgtgctgcgc gcctccctgg cgccgcacct
    11401 ggatcgctat gtccggctgg cggcgggttc gctgccgccg gagggagccg tcgtgttcag
    11461 cgaggacccg attcacctca agctcctgtg cgcggcctgg ggccgggaag gcagcgcgaa
    11521 tcgttatctg ccggtggacg cgaacagcct gccgctgcag ccgtaccgcg actggttgag
    11581 ccggaaatac cccggccgct ggccggcctc tcccgcgtcc gcgcagcttc tcccggatta
    11641 caacgtgccg ctgaatgctt tgcgccaggt gcaactgctg gcccgcgtgg cccaaagcaa
    11701 ccggctttgc tgtcttccgc ccagctacgg gccgctgctc aatcatttct acccgcagtc
    11761 ccagggcctg ctccacgaaa tgaagcccta tccccgcgac ctcctcagcc ccccgccgct
    11821 gaccgcggag gaactggcgt ccggccaggc cttctggcag caggccatcc cggcggccgt
    11881 aaaacccgtg ctgcaactgg tctccaccaa tgaactcccg cgctccggat tttcccggtg
    11941 gctgctgaag atggcgcggc tccaaacacc gccgcccgtt aacgccaggc tgctggccgg
    12001 gtggtattcc cgcgccctga actattgggg cgttgaactg cagcgcaacg gccaggaaca
    12061 ggcggcgacg ccgtgcttcg agctggcccg ggacttaaac ccggacaacg ttcccgcccg
    12121 gatcaatctg cagtgcaacc acaacctgcg ggcgcgccaa ccgttgacga tcgccgacga
    12181 cacctcgctg gaagagcagt tcggcaaata ccgcaatatc gtgcaactgc tggccgacaa
    12241 cggcccgttc gacgagccga atgcctgctt caaactgggc ctggggttcg cccacaccgg
    12301 cctgctgcgc caatcctgcc agcaattcga gcgggtcacc caactggtgc cggatttcct
    12361 gcccgcgcgc ctgttgctcg cggatgtttg cgccgaaggc ctcagccccg gctgggcgct
    12421 ccaatgggtg aaggaaatta aagccaaccc cgcctggcag ccgctcgggt tgaccaacga
    12481 ggtgaccctg gcgctgacgg aagccaaggt gtggttcgcc gtcaccaacc gccccaaagc
    12541 gcacggaatc atccacgcac tgctggccac ccaccccaac aatccctttg tgtacgaccg
    12601 cgccctcgcc gcctttatgt cgcagggaag ttacgcggaa gccctgcgcg tcattgacca
    12661 gcgcctgcag cgcgatcccc tggacgccaa ttccctgtcc gaccagggca atctgtacgt
    12721 gttaatgcac cagttctcga atgccatccc ggcgctgacc cgcgcgctgt ccctgaccaa
    12781 ccgctatgcc acccgccgtt ccctggcgct ggcctatctg cgcaccggcc agttggaggc
    12841 ggccgaagcg gaatgcaaca ccctcctcaa aacctttccc gacgtttaca gcacctactc
    12901 cgggttggct gaaatcgctt tgcagcgaaa ggacaccaat accgccatcc actattacga
    12961 gcgccacctg gccgccgccc aagcccgcgg ggacgaaacc cggcatgtca ccgagatgct
    13021 gaaattgctc cagccggggc gccgttgacg ccgccctccg gcggcggacg tgctgaggga
    13081 aaggccgcgg cgggccggac gtttccctat cctcggcgga aggggtcagg gccggacggc
    13141 gacaaaggaa accgcggtca ggtggcggta atgtttgcga aacatgcgcc gcatggacag
    13201 cacgcgcttg cgcgcctggg gccggcggag gacgttgaag acaaagcgca gggcgctgag
    13261 ccagccttca tcacgaatca ggcggcgcgg ctccagcaag tgcatcggca cgacggcttc
    13321 cgcctggatc tggaaacccg cgccggtcaa caactcgcgc cactcacgca gcgccagcgg
    13381 gcgggcgccg acgtggatat tgcgggacat ctcgcggctg atgtcctcct ggatgtcccg
    13441 gggcaaatcg tccggcgtaa agcccagttc gtgaatgccg tagcgcccgc cgggcttcag
    13501 caggcgaaaa gcctccgcca cgatcttccg cttctgctcc gccccctgca tggtcagcat
    13561 ggcttcgccg tacacgacgg tggcggattg gtcgtccagg ccggtgcgct cggcgttgcc
    13621 caggtggcaa cgctggttcg ggccggtcag gtagcttcgc acctgggcgg cggcggcctc
    13681 gtcccgttca atggcggtgt agctggccgg attccggctc aacgtcatcc gcgcggtcac
    13741 gcccagcccg ggggcgaact ccacgaccgc gtcgcgcggc tggatgtcga gctgctcaag
    13801 catgcggcgg gtgagttccc gccctccggg gcgcaatacg cgcttgccca tccgggccag
    13861 cagccaatgc cccggggtct tctccggatc attcaggggc gagcaagggc tcagcccgtg
    13921 ggttgccgat gatattgttg atgaggccac atggcaaaat aatggctccg gcaaataaag
    13981 caagttctac tgcttattat tagctttggt gcatggcggg gacgccgccc ggcgcgggcg
    14041 ggggaggcgg caagcccgcg ttccggccgt ccggccgcgg ccggatcaag gccggggcgc
    14101 ccgccggcgc accttgcgtt tctcctttcc tccaaccgcc ccaccgggtt ttggccgccg
    14161 ttgggggcgt tcggtggagg gcgggctttt taaggctcga tcttcagcag caccccgcct
    14221 cccggcgcca gccagacgtc ttcgccccgg aagggcgcca gcgcgccact gtaaggcgag
    14281 acgagcgaca cctggcgcgg cgcggcgcgg aattgcagcg agcagatgcg cgatttggcc
    14341 aggtctttgt tcacgaccat caggtagcgc gagccgtccg cgtgggtgaa ctcgccggcg
    14401 agcacgcccg cgccgccggc gccggtgagc aggctgtggg cgggcgggcc gctggtgccg
    14461 gcgggaagcg gcccgaaatg atacacggcg tcggacttaa gccgcaggag cgtgggggcc
    14521 agtttctgaa cctggaggtt gatgtgctgc atgaaatacc aggtcggcgt tttgttgccg
    14581 aactggtcta tcggccccat ccggtaattg cccgtgctgg gcgtgaagta ggtgaaatag
    14641 gaaatgccgc gcccgccgta ggccagcgtg gtatagacct ggaaccggaa gtcggcgccg
    14701 ttgagttcgc ggtagctgaa atgggcggcg gccagcacga tattccagaa ctccaacccg
    14761 tgccgctggc aggcggcgcg cacggcttcc aggttcgacc agtagctctc gcgcaccgaa
    14821 ccatcgtcca tcagcgagta attgtcgtag ctgataatgg ccgggcggca ggtggcgatg
    14881 aaacgctcca ggtaatccgc gtagctggtt gcgccgagct gctccgcgct ggcgtagtcg
    14941 gggaacaggt tgatataagg ccatttgccc ggcgacatct cgcggatgag gtccgccacc
    15001 ttggccagcc ccgggaacat cgcggcattg ggttcgtccc gcaggtaata gccataaacc
    15061 gccgggtgcc cgcccacctc ggcgaccagg ctggcgatat tcgttcgcgc ctttgcctca
    15121 tccacgttgg cccagtcgta gccggacgtg cgcgcatccg agacaatcgc cttgaggccg
    15181 gccgcctggc acgcatcgag tgtgtccggg ttaacgaacc cggcgaccgt tagcccgccc
    15241 tcccgcatcg tctgcagcac agccgggtcc ttcggggccc agttccaggc catgatgggg
    15301 aagaagttgg tgtcggcggg cgccggggcg gcataagcgt atccggacgc gagcagcagg
    15361 ccaagggcaa caagcaaggt tctcatgcgg ggcactatta ccccgccggg ccggccaagt
    15421 aaaccccgca atccccggcc aacaccgcga ttattcgcgc ccccccgccg tgtcaccata
    15481 attggggaca tgacgaaaaa gggggttctg ctatcttggc attgtcagtg atgcaaatag
    15541 cgcagtagtc agaccgtgtg tgactgacag gcgtaaatct gcggcgccgc atcaaagacc
    15601 gatttaaagt tgttctcgag tagaatgtgg cagaaaagcc ggaagtaact tccggctttt
    15661 ttgttgtacc gcggggcggc cccaaccgga gggcgaaagg ctgccgccga aaggcgggca
    15721 ctggaaggcg ggaagcgaac ggcgttcagc ccgatccgcg gggcgtccgg gcgcggcgca
    15781 gcgagcgcag aatctgcaag ccgaagcgtt cgtcgggcgt cagcacccgg ccatgggcct
    15841 ggagttcccg cagggcggcg agggtcatgc aaaagtccac cgcgctcagg gggcggcccc
    15901 gcacgacgcg gttgtgccag cgctcagcct cggccacttc ctcgatttgc tcatcgccgc
    15961 ccgggaagaa ggaaatcagc caatcatccc gccggaggta gccatcggct tccgtcttgg
    16021 gctcgaacca cgggtcgcac aggagcaggc aaatggaacg cccggcgttg gcgccgaggc
    16081 ggtagaacac gagccgcgcg tcgttttcgg cgaaatggaa ataggtcttc cgggcggcat
    16141 cggggggctg gggggtgaac cgggtttgga agccgagctt gacgcgggcc gtcgtccccg
    16201 ggtctttctc gaagcagaga ttctcctcga ggatccagag gaggtcggtc gagcggccgt
    16261 gctgcgtcag aagggttttc caggccgcca aagcctcttc aaacggaggg cgcagataac
    16321 gacgggtgtt gtccataatg attattagtg gggctggcgc gtgaaccagt caataaaaag
    16381 cgggatgccc tgctcgatct gcacgcgcgg gtggtagccc agcccggccc gggctttggt
    16441 gagatcggcg caggtgaggg gcacatcgcc gggctgcggc ggccggcggt cgaggatggc
    16501 ccgtttgccc agggcgtctt ccagcagggc gacgagctga tccagccgca cggtctggga
    16561 ttcgcccagg ttgaagattt catagccaaa ggtcctttgc gtgcaggcca ggatgccgtc
    16621 gagaatgtcg ctgatatagg tgtagtcgcg ggccgtggag ccgtcgccaa agacggggac
    16681 gggctggccg gaatggatca gccgggcgaa cttgtgaatg gccaggtccg ggcgctggcg
    16741 cgggccgtaa accgtgaaaa ggcgcagcat gacgatctcc agcccataga catggtgata
    16801 aacgtggccc agcgcctcgc aggccagctt gctcgccgcg tagggcgaaa tggcgcggaa
    16861 gatcgggtcg ctctcggcaa aggggacttt ggcatttacg ccataaaccg aggaggacga
    16921 ggcaaggatg agcttgcgga cgccgtggac ccgcgcggct tccaggagat tcaccgtgcc
    16981 ctcgacgttc acgcgctggt acagggcggg ctcttcaagg ctgggccgca ccccggcgcg
    17041 ggcggccagg tggatgacct ggtcaaagcg cacccgcccg agcaggtcgt ccagcgccgc
    17101 gcggcgggtc aaatccccat ggacaaactc gaagcgcccg gccagcgccc ggaggtcgtc
    17161 aaggttgcgc tgcttgaggc gcgggtcgta gaaagggttg agatcgtcaa acgcccagac
    17221 ggcatgcccg gaacgaagca gtcgctcgca gacatgcgag ccgatgaagc ccgcgccacc
    17281 ggtgaccagg aaattcaccc gcgcacgcta ccagaacggc ggggggattg tcgagcctcg
    17341 ccgcccgggg ttcccggcgc attttgcgct ttccgcgcgg cgcggcgcca cccatattcc
    17401 ccgccatggc aaccgaaccg ctgctccaac tggatttacc cggcatcccc cgcctgaaaa
    17461 gcggcaaagt gcgggaagtc ttcgacctgg gcgacagcct gctctttgtg gccaccgacc
    17521 gcatttccgc gttcgattgc atcatgccca acggcatccc gcgcaagggc gaggtgctga
    17581 cccagctctc gcatttctgg tttgcgcaga cggcggcgtt ccagccccat cacctgctcg
    17641 cccgcgcgga agaccccctg ccgccccgcc tccagccgta ttcggcgcaa ctggcccgcc
    17701 gcagcatgat tgtcaaaaaa gcccgcccgc tcgccatcga atgcgtcgtg cgcggctacc
    17761 tcgccggctc gggctggaaa gaataccgcg cgcggcaaac ggtctgcggc ctccggctgc
    17821 cgcccggctt gacggagtct tccgaattgc ccgagccgat cttcaccccc gccaccaagg
    17881 ccgaatccgg gcatgacctg aacatttcct tcgccgaagc cgcccgcctg gtgggcgccg
    17941 gccgcgcgga gcaggcccgg gccgccagcc tcaaactcta tgcgttcgcg cgtgattatg
    18001 cgctccgccg gggcattatt atcgccgata ccaagttcga gttcggcctg gtggacgacc
    18061 aactggtcct gatagacgag gttctcacgc cggattcctc ccgcttctgg cccgccgacc
    18121 agtatcgccc cggcgccggc cagcccagct ttgacaaaca gttcgtccgc gactacctgg
    18181 aaacgctcag ttgggacaaa accccgcccg cgcccccgct cccgcccgaa gtcgtcgccc
    18241 gcacgcaggc caagtacctg gaggcctacg aacggttgac cggccgcaag ctggcatgaa
    18301 ccaactggtc gaggacttcc tgcaatacat ccgccacgaa cgcggccagg ccgagcacac
    18361 ccagaaaacc tacgccctgc tgctgggcaa attcatcgcc tgggcaaacc agcgggggct
    18421 gtcggactgg aagtccgtcc agctcgcgca cctgatggcc tttctccagc acgaacgcga
    18481 gcgcgccctg gcccaccagc ccccgggctc tccccggcgg ctcagcagcg agagcctcta
    18541 cctggaaata gccgccctgc gggcctttta ccgctttgcc gaaaacgaga aactcctgcc
    18601 ggccaacctg gcggaaaacc tttcgctgcc ccgccgctgg cagcgcctgc cccgggcgct
    18661 caccggccag gaaatcgaac ggctgctcac cccggaaacc ccggaaaccc cgcaaagcct
    18721 ctgcgaccag gccatgctcg aactcaccta cgcctcgggc ctgcgcctgg ccgagcttcg
    18781 ccacctgcgg ctcgaacaac tccatctcgc cgccggattc atcacggtca tcggcaaagg
    18841 caacaaggag cgtgtggtgc cggtgggccg cccggccgtg gccgccctgg aacgctacct
    18901 ggccgccggc cggcctcaac tggtcactcc ccgcaccccg gccaacgtgt ttctcacccg
    18961 ccgcggcacc cctttcgcgc ccgtgaccct gtggttgcgc atcaaacggc gcgcgcgccg
    19021 ggcgggcatc gcgcgcaacg tcaccccgca catgctgcgt catagcttcg ccacccacct
    19081 gcttgaccac ggagccgacc tgcgcgtcat ccaggaactc ctcggccacg ccagcatcag
    19141 caccaccgag gtctataccc atgtggcggc aacccgcctg cgcgaagtcc atcggaaata
    19201 ccatccgcgc tcttagccgg aaccactcag ttgggcagcc caaccgcgcg ttttcgtcct
    19261 gttggggcct gggtggctat ggttatcccg cccgcaaccc tcataaacct cttatttaac
    19321 gggtttaacg gctcaaccgt tggcctctga atggatttta cctgtaaaat cagtagttta
    19381 ggcatgcgga agggttgaaa tagcggtaaa atccaaatcg ttccggcttg gatttaccct
    19441 gcatttaccc tgcttctacg ccgtgtacaa ggggtgtaca agggatgccc atcgggtgta
    19501 ccagctaaaa tcccatgaac ccccggtggg catcccttgt acaccccttg tacacggcct
    19561 gggggaaggg tattttcacg gtatctgggt aatggatgag ggtgcctgga aaggccgaaa
    19621 cgatgagcga gagcggagtg acgagcaata agtccaataa aagcagttag ttataaatct
    19681 gaggctaacg ggagaaggtt ttttagtgac ggacgggcat ccggatggct tttgacctgg
    19741 cgaatggctt tcacttgtaa aatcagagat ttacacgttc tgtgggatcg aattggaggt
    19801 ggaatctgaa ttgtttcggc ttaactgaag cgatttaggt gggcggtcta acagcatgat
    19861 ctcgattcat cggggcttga gaggacatgg tcgcttagaa tgtagtgcct gcgaaccttg
    19921 taattggtga atccagcggc tttgccgcca gtcttgggcg cctttcaacg gtgaaatcag
    19981 taatttacag gggcaagtag gcaggacagg ggcggggttt gaggcgtttc ggcctggtca
    20041 ttcatccact tcttttccac tggtcaacca ggcggttgtt ggattatccg gcctgaccgg
    20101 tgcgcgattt tgcgagccgg tctttgggga tttgggcagt cccgtcccca ttcgcgccgt
    20161 tccgggtggt atgccggggg gcgcgagcga tgcccgcgcc ccggcggcgg agtggggcgc
    20221 tgggcgtcag cggctgacga tttcgggcag cttgaagatc ggcaggaaca tgcaaatgac
    20281 catgccgcca atgacgacgc ccaggaagac aatcaggatc ggctcaatca gcgccgtcag
    20341 gcccgacagc gtcgtttcaa tttcctcgtc caggaaatcc gcgatgcgct ccaacatgct
    20401 gtcgagcttg ccggtttgtt cgccggcggt gaccatgcgc acgatcatgc tggggaatac
    20461 cgggtgcttg gccagggcgg tggcgatgct ttccccctgc tcgatatccg tggaggtggt
    20521 tttgatggct ttctccatga ccacattgcc cacggtctgg gatacgatct ggagcacttc
    20581 caaaatcggc acgccgctgc ggacgagcga agccagcgtg cgggcgaacc gcgccaggca
    20641 aatcttgtgc gcaatcgccc cgaagaccgg cagcttgatg cggcgcgaat cccagatcca
    20701 ccggccggtc ggggtcttga tgaaatatat ccagccataa accgcccccc cgccggccag
    20761 caggacgagg aacagatacc gctttacgat gtcgctgacc atgatcaggt acatggttgg
    20821 cgccggcagg gtggcgttga agctccggaa gatttccccg aacaccggca ccactttcac
    20881 cagcagaaag atcgtgatca gaattgccac gaccgtcacg acggccgggt acatggttgc
    20941 cgatttgact tttttgcgca gccgggcggt gttttccagg taggtggcca gccgggcgag
    21001 gatctccgcc agcaagccgc ccttttcccc cgccgccacc atgcagacgt agaggcggct
    21061 gaacgccttg ggatgcttct tcaaggcgtc ggaaaagctg tcgcccccct ccacgcgcgc
    21121 gcatacgtcg tgaatcacgt cccgcatcac cttgttggtt gtctgctccg ccagcgcctg
    21181 cagggattgg accatcgcca ggcccgcgtc aatcatcgtg gccagttggc gggtgaaaat
    21241 taccaggtcg gccagggcca cccggccgcc gctggtccgg ccttgtttcc ccacccgctc
    21301 ctgaatcgaa agcaccagca tgttccgatt cagcagcgcc atgatcgccg cctgctccgt
    21361 cgccgcttcg accgagctgc gaatctcccg gccggaagca gtttcccgcg ctacatagac
    21421 aaaagaagcc ataatctggg tctgttcagt cttaaccggt tccgccctta accagccggg
    21481 gcggatgata tcgagttgcc atgcatttgc tgccctccaa cggagcagca tttgttccaa
    21541 acgggcgggg atggctgtcg gggatggtgg cggcggcgga atccgcgggg cggcgttgct
    21601 ctcatcggcg gtttggggcg ccggttgggg gagtcttccc gggctcgatt gcctcaattt
    21661 gacacaattc ccgtcctcaa ttacagacaa gctcgcgcgc caaaatagta aaaatagttg
    21721 ttgtcaacgg aactgaaatg cactatttag tgacctagat gcaccggttt tggtgcgagg
    21781 gcaaaccggt tttgccctca gaaatgcata gtcatagtta tacaacaaac acgagtcata
    21841 tcggaggaac atatgaagta taataaatgg acagtagcgc tggccgccct gggggtggtc
    21901 agcttggcat ccgctgccaa agccgaggaa tgcttgagcc cggtgaaaac cgcgctggct
    21961 tccacgacca tcagtggata tgtggatacc tcagcacagt ggaactttgg caccggcaat
    22021 tacaacctgc cgccctataa gttcgggggt attgataagg ccgacggctt caacctgaac
    22081 gtcatccagt tgcgcatcga aaagccgctg gacgagcagg attgggcggc tggctatcgc
    22141 gccgacctgt ggttcgggcc tgatgccaac cggctcaaca cccagtcctt ctggggtgag
    22201 ggcaatcgcg ccgggacggg tgatctcgcc gttcggcaag cctatgccgc gctgcgcacc
    22261 ccggtgggca acggccttga tttcaagctg ggcgcattcg acagcatcat cggctacgaa
    22321 tccattgaat ccggcaataa ccccaactac acccgctcct acggccatac catcgagccg
    22381 caaacgcata cgggcttgct ggccagctat cgcttctgcg actgcttcag cgcgtccgcg
    22441 ggcgtggcca acacgtacgg gccggctatc aacgagcggg cctttaatgg ctactactgg
    22501 agtgacaccc gggcagaatc ctacaagact tacatgggct cggtcgccct gaccgctccc
    22561 gacagttggg gattcctctc cggctcaacc ctgtatggcg ggattgtcaa cgggttcggc
    22621 ggcgaaacgc tgaccagcta ttacgccggg ctgaccgttg ccacgccggt ggagggtctg
    22681 cgggcgggag tggcgtttga ccagattaac atccacgatg tgaagaatga taactgggca
    22741 atcgccggct atctctccta ccaggccacc gagaagctca gcctccacgc gcgtgcggaa
    22801 tactacaagg acaaggtggg caatggtggc acgctgctct tcggccgcag ctttgagacc
    22861 gaagacggga ctgtctgggt gggaccgcgc aaagccgaag tcctcgccct cacggccacc
    22921 gtgcagtatg acctgtggaa gaacgtcatc agccgcctgg agctgcgctg ggatcattct
    22981 ctggacggtc acctgttcgg cggcaagagc ccgaattctt acgatgcgga cacggacacc
    23041 tacacgagtt ccgccaatct gaagaacgcc tggatgctgg ctgccaacgt catctacaag
    23101 ttctaacctg agttagaatc aacgtttgag accccctccg ggtgaactgg agggggtttt
    23161 tctttgtgaa gcgcccgccc cggatttcag ctccgggctg aatcagcggc tccgttgtcc
    23221 gccgccggcg tgcggatcaa tcccccagca gctttcggag ggtgtcgcgg gctttggctt
    23281 cgcgcgaagt atcctcggcg ggcgggacat actcgcggcg ggcgaactcg aagtattcgc
    23341 agtaattggc caggtgcttc tccgcgacca gctcagcgcg gcggtcgcgg cactgctcgg
    23401 caacccgtgg atcgtagctg atgcagttgc ggcacacccg gaggtccgag cggcagcgtt
    23461 cgcagcattc cgagcggccg ggcgttcccc gcagggtgta ttcggtgccg catttccaac
    23521 aatgtcgggt cagggccatg cggttgagta taaaccggcg ggcgcacaat gccagcgagg
    23581 attggcggcc gtccggctgg cgtcggtcgc tatttcgttt taggttccgg cgcccgggtt
    23641 ggcgcgtcgg cgtccttgag aggccagtgc tccgactcag tccgcgcggt tttcagataa
    23701 tcctcaatcc gggccaccac ctgcgggttc cggtccgcca cgttctcctt ttcgcccgga
    23761 tccgtcttga gatggtacaa ctccagcggc gccccggcgc gcaggcgcac cgccttccaa
    23821 tccccgtaac gcaccgcctg ttggaatccg cgctcgtgga attcccagta aaagaactcg
    23881 tgggggttgg tctgcggctg ccccagcagc gtcggcagca gggaccggcc gtcgatgggc
    23941 tccggcaccg gggcctgggc gatcgcggcg gccgtcggca gaaaatccca aaaggcccac
    24001 ggctgatcat tgcgcgcgcc gggcttgatg cgggcgggcc agcgcacgat aaacggcacg
    24061 cgaatgccgc cttcgtacaa atcgcgcttg agaccgcgca gcgggccgct gctctggaag
    24121 aactgcggat ctacgccccc ttcccggtgc ggcccgttat cgctggcgaa gaagaccacc
    24181 gtgttatccg cgagcttcag ctcctggagc ttgtccagga tccggccgat atcggcgtcc
    24241 atgcgcgtga tcatggccgc cttgttcttc tccggcggcg gccaggactg gtcggtatat
    24301 ggggcgtcgc tggggacttg cataccgttg cccgtgcggc ggccctcttc gttgttggcg
    24361 tgcggaatgg tgtaggcgag ataaagaaag aacgggcgat agtggttgaa ccgatccggc
    24421 tggttgatct ggataaagtt cagtgccgcc gtggtgaact ggtcatgcac gtaaagcccc
    24481 cggcggccgc cttgattttc gaggaagtcc cgccggccgt cgtagcccgg tttggccggg
    24541 ccgggcgggt cgtagcgcca caggtaatcc gggtagtaat tgtgcgcgtg gacctggtcg
    24601 agaaagccaa gaaattcgtc aaaacccttc ttttgcggca ccccggttgt gtgttcattg
    24661 cccaggcccc acttgccgat gaggccggtg cgatagccgg cccgctgaag aatctcggcc
    24721 accgtcagat cttgcgggcg cagcgccacg gcggcgttgc cgcggacccg cgcatggccg
    24781 gtatgcaacc cggtcatgag cgccccgcgc gagggcgcgc aaaccgtgct gccggcgtaa
    24841 aactgggtga accgcatgcc ctcggcggcc agccgatcca gattgggggt ctggatcttc
    24901 gtctgtccgt agcagcccaa gtccccatac cccaggtcat cggcaagaat gaaaataatg
    24961 ctgggccgcc gctggttggc attgggcgaa ctgctcaccg ccgcaccgta actcgaaaaa
    25021 gcagccgcca ccgccagccc cggaatcaga acccgattca cgcaccgcca gtttccccaa
    25081 aacctgatct tcataagcca gaatctggaa cagaccttgc ctgccgtcca cccaaaaccg
    25141 gtccgtgcgc cgcccccctg cggggatgcc tccgctgcgc tgccggtcgt gtcgtccgta
    25201 gcgaaagagc gtcgcccggc ggcggcgcct tgcgcgctgg ccggggtggg cggcttgcgg
    25261 gccgttagca cccgttgccc ggggcggggg cgtatttttc cgcggtttgc cgggggttgg
    25321 agcgcgtccg ttcatgttgg cgcgtgcggt tcgttgacac ggattggcgc ggctgttagg
    25381 gttgctcttc tttaagacgc tgcatggatt acaagaacac attgaacctg ccgcagaccg
    25441 atttcccgat gaaagcggat ctcatcaaac gggagccgga gcggctgcgg aagtgggacg
    25501 cggcggggct gtatgccgcc atccaggccc ggcgcgccgg ggcggagaag tttgtcctgc
    25561 atgacgggcc gccgtttgcc aacggcgagg tgcatatcgg cacggccctg aacaagattc
    25621 tcaaggacat cattatcaag tacaagaccc tgcgggggtt tcatgcgccg tatgtgccgg
    25681 gctgggattg ccacgggttg ccgatcgagt tcaaggtgat gcaggacatg cgcaaggcgg
    25741 gcaattccgc cacggacgcc gcgtccatcc gccaggcctg tgaaacgtac gcgcgcaagt
    25801 atattgacct ccagcgcgcg caattccagc ggctgggcgt gctgggagac tgggcgaacc
    25861 cctacctgac gttcgcgaag gaatacgagg ccgacgagct gcgcctgttt gcggggctgg
    25921 tggagcaggg catggtgtat cgcgggaaga agccggttta ttggagcatc ccctgccgca
    25981 ccgcgctggc ggaggcggag gtggaataca aagaccacgt cagccagagt gtttatgtga
    26041 aattcccgct ggtggggcac ccgggcacgt cggtggtgat ttggacaacg acgccctgga
    26101 cgctgccggc gaatttggcg gtggcctata attcgacgtt tcactactcg ctggtgctgg
    26161 tcgggcggga gcaatacatc gtttcggccc tgctgctgcc ggcgatcgcc gccaaatgcg
    26221 gctggcagga ttaccagatc gttcgcagcc tcgagggcgg gcacctggcc cagatggaat
    26281 accagcaccc tttctgccgc cgcacgggga agtttttcaa aggcgacagc tttgtggaca
    26341 acaccacggg caccggcttt gtgcatattg cgcccgggca cggcctggag gattatcacc
    26401 ttggcatcga gcagggcttg ccggtttatt cgccggtgga cgacgacggg aagtttgtgc
    26461 atacctccga tctgccggcc gagcagcaga tgccggccga aatgctgggc cggtccgtcg
    26521 gggaaaagca cggcaagagc gaggcgaacg aagcggtgct gcacgaattg cgcgcgcgcc
    26581 aagcgctgtt gcaccaggag gattatacgc acagctatcc gcactgctgg cgcagcaaga
    26641 ccccgatcat cttccgggcg atggcccagt ggttcatcaa gatagaccac gcgggtttcc
    26701 gcgatcgggc gctcgccgag attcaacggg tgcactgggt gcccgaatgg ggccgcagcc
    26761 gcatcgaggc ggccgtcaag ggccggccgg actggtgcat ctcgcggcag cgcacctggg
    26821 gcgtgccgat tccggcgttc tatgatgcgt cgggcaaccc gatcctcgac ccccggatcg
    26881 tgcgcaacgc cgccgacctg ttcgagcagc acggctcgaa tgtctggttc gagcggtcgg
    26941 ccgccgagct gtgggccgcc ctccagccgg cggactggac cggcccggcg tccgtcgcca
    27001 agtccaacga cacgctggat gtgtggattg actccggctc ctcttcgcgg gcggtcattg
    27061 cgcggcggcc ggaaatccgc ggggccggcc acccgttcca gtgcgacatg tatctggagg
    27121 gcagcgatca gcaccggggc tggtttcagt cctcgctgct gttgtcgctg gcgggcaacg
    27181 gcgccgcgcc ataccggacg gtgctgaccc acgggttcat ggtggacgcc gaccgcgaga
    27241 agatttccaa gagtcagcaa gggcagggcg gttatgagaa gccgcagacc gccgaggcgt
    27301 acgtaaaaaa gtggggggcg gatattatcc ggctctgggt tgcttcgcag gacttccgca
    27361 atgacatcat cgtcagcgag gagcgcatcc agacggtggc cgagacctac cgcgttctgc
    27421 gcaacgcgct gcgctatgaa ctggccaacc tcgccgactt cgacccggcc gcccaggcgg
    27481 tgccggacgg gcagttgacg ggtctggacc gctggattct ggacgagttt tcgcagttgg
    27541 agcgggacgt tctggcagcc tacgaccgcc atgagtttca cgtggtgtat cagcgggtca
    27601 gccagttcgt ggcggtcgag ctttccgccc tttaccatga tgtggtgaaa gaccggcttt
    27661 ataccgatcc ggccaacgcc ccgcgccgcc gctccacgca aaccgcgctg taccggctgg
    27721 tcaccggcct ctgccgcctg ctgtcgccta ttttggcgtt cacggcggac gaggcgtggg
    27781 agtttgtgcc gggccggacg gttccttcgg ttcacctgct cacctgggag ccgctgggat
    27841 tcgagcgccc ggaagacgaa cgcgccgcct ggcggacgtt gctggagctg cgcgagcggg
    27901 cgctgcccga gttggaaaag gcccggcagg ccaaatccat tggcaaggcc ctggaagcga
    27961 agctgacctt tgccggcgcg gggccggagc tggcaaccgc ccaggcgcac cgggaatcgc
    28021 tgcgggagtt gttgaatgtc tcccagctcg aaatccagcc gacggcggaa gcggccctga
    28081 cgatttccct tgcgcgggcc gcgggcgaaa aatgcgggcg ctgctggcat tgggaaaccc
    28141 atgtcggggt ggacgccggg catccaaccc tgtgcgcccg gtgcgcccag gccgtgcggg
    28201 aaagtgtgcc ggcctgaccc ggcccgtcat ccccgcgggg gccccttcgc gttcttcccc
    28261 gcccgccgcc gcgccgtcac gggcgggtgc tttgacccgg gaaacggtag atggacgcgg
    28321 ccggttgact attgacttac ttggggtcgg gagtaggctg tttttatgcg tcaggcgcct
    28381 caatgggaca cggtttgctt ctcgggaaag ggcccggcgg tccatttgcg ccggcggccc
    28441 gcctgaacgg aaaggacact atgccccagg ccatcatgga tcccgagcag gtgcggcgct
    28501 ttgccgagga gctcaagcgg ttcaaccacg atctgaaaga ccggatggta tcgttgcagg
    28561 cgcggtttgc ggcgctgggc gacacctggc aggaccagga gcacgccaaa ttcgccgagg
    28621 agttcaagca caccatgaag gcgctgcgcc agttcgtcga ggtttccaat cagcacacgc
    28681 cctacctgtt gcgcaaggcc cagcgcatcg aggaatacct caaccagcgt taacccatgg
    28741 ccgagcgcgc gcaggtcacc tcgtcggacg cgctggagtt attccggtcg aacctgctgc
    28801 tttacctgga caaggcgcgc ccggcgctgg acgaggtgag cgccgaagtg atgcggatgc
    28861 gcaactggct ggaggacgag cagcgcgtgt attgggaaag ccaggtgcgg cggcggcggc
    28921 gggagtttga cgaggcgcag caggcgctgt tcagcgccaa actgtccctc ctggaccggg
    28981 ccagctcggc ggagcagatg atggcgcatc gggcccggcg cgcgctgcag gcggcggagg
    29041 aaaagctgcg gaaagtgaaa caatgggacc gcgtgttcga gagccggacg gcgccgctgg
    29101 tcaagcagat ggaaaagctg cataccgtgc tggcgcacga catggtgcag gcggtggcgt
    29161 ttctgacgcg gacgctcgag acgctcgagg cgtatcggcg cgtggcgccg cccgcgtccg
    29221 ccggggccac ccgcgccagc ggcgaaaccc catcgccggg aggagcgcca tgaaaggcag
    29281 cggcgcgcgg ctgggcggcc tcaccaagga gttgcgggcc caatggctgg acacgaaaag
    29341 ctattggcgg gacgcccggg gcgaggagtt cgagcgccgc tacatggaag aactctttgc
    29401 cagcgtggac cgggcggcta ccgtgatgga gcaattggac aaactcttaa cccaaatcag
    29461 gcgggattgt gagtagcaac ctgggcgtcg gccaaacact cgaactgctg gagcggctcc
    29521 aggccacggt ccgggctttt gccgcccggt cggagaagct caacgcggaa taccatgcgc
    29581 agcggggccg ggaacagcgc ctccgcgaag cggtgcgcga gcggcaggcg gaggaactgg
    29641 cagcggctga ccgtgaggcc gaagcggcgc gggcggcggc cagccaggcg cttgccgccc
    29701 ggcacgcggc gcgcaaggcg cggatcggcg ccgcttacca ggccagcaaa gagcagcgcc
    29761 tcgcggagat cgaggcccgg accggcgcgc gcaaatacga actgcaaaag gccatgctgc
    29821 aagccgagcg tgaccgggac gcgggccagg ccgccttcgg gacggctttc gaggagttca
    29881 ggcatctcct ggccgccgag caggcggagc tcgccgcccg ggaaaccgag gcgcggaagg
    29941 ctttccaggg ctatcgcaag ctcgtccgcc gcctggccgc cgccgaccgg aaaacgcccg
    30001 cgcccgcgcg ggatgaacag caactgctgg cggaattgcg cgaatgcctg gcgcagacgc
    30061 gggatgatct gcgccgcttc cgcaggttcc tgccgctgcg ctggttcaaa tccctgccgc
    30121 tatgggggct gatcgccctg gggctggtcc cgttgctgct ccggcagttt ggctcggggc
    30181 ccgccgctta cgggaaggcg ggcctgggcg cggtgggggg gctggtggtc gtcctgatca
    30241 ttcgcagcct ggcgcagcgc cgggttcggc ggcaggccgc ggccatcgcc gacgggctcg
    30301 cgcaggcgcg atgctgccat gacgccgccg cggaacgcgc cgaggcgcat tatcaacagg
    30361 agctggaacg cctcggaagc gagtttgcga acaccacgca aacggtggac caggagttga
    30421 agcgggtgct ggcggaggcc agcgaactgc gggtggcctg ccgcacagcg agcgatgaaa
    30481 aggcggcgcg cgccctggcg aggaatgatc acctccagcg gatccggcag gagcggcagc
    30541 accggcagca ggtggaggcc ggagaacaac gccggcggac ggtggtggcg cgcggccagg
    30601 cggagacccg ggccagcgag cagcgggagg ccgcgctcaa cgcggattac caggccgcgg
    30661 gtcaggcgct gacggccgaa tggcagcgcg cgatcgggcc gctctacgcg gatatccagt
    30721 cccttaacgc cctggctgac aagctgttcc cgccgtggca ggcgccgggg ttcgaggcat
    30781 ggacgccgcc cgcccagttt gcgggcgcgg cgaagttcgg ccggctggag gtggagatgg
    30841 aaaagctcgc gccggcggcg ctccgggacg ggcgcctggc gtggcccggc ccggcccggt
    30901 tctcgctgcc ggcatgcctg gcgtacccgg gccagggctc aatcctgttc gagacggccg
    30961 agcagggccg cgacgaagcc attggcgcgc tgaacaacct cctgttgcgg ctgctggcca
    31021 ccgcgccgcc cggacggctg aacttcacca tcattgaccc ggtggggctg gggcagaact
    31081 tcgccggcat catgcacctg gccgaccacg aagcgcagct catcaacagc cgcatctgga
    31141 cccagccggg ccagatcgag cagaaactgg ccgacctcaa tgagcacatg gagaaggtca
    31201 tccagatgta cctgcgcaat gaatatgcca ccatcgccga atacaaccag caggcggggg
    31261 tcattgcgga aaagtattat ttcctcgtcg tggccgactt cccggcgaac ttcacggaaa
    31321 ccgccgccaa acgcctgctg agcatcgcgg ccagcggggc gcgctgcggc gtttatacgc
    31381 tgattcattg ggaccggcgg ctgacgctgc ctcccgagtt catcccggag gaattgcgcg
    31441 ccgacagcgt cggcctgagc gccgttccgg acggccgcct gtccccgcgc gcccaggccg
    31501 cggcgatccc cggggataac cgggcgcgcg gagacacccg ccggtccgag cccgccggcg
    31561 gcggcggggc tgtcttccgc cttgccggcc cggcccggcc gggcatccgc ctggaactgg
    31621 agccgccgcc gccgccggag ttcgccatcg agttcgtgaa ccgggtgggc gtgagcagcc
    31681 gcgattccag ccgggtcgag gtgcccttcg cgcatgtggc gccgccggcc gcggagattt
    31741 ggagccagga caccgccaaa gagctgcttg tcccgatcgg gcgcaccggc gcgaccaagt
    31801 tccaatacct cgccctgggc aagggaacct gccagcacgc gctgattgcg ggcaagaccg
    31861 gctcgggcaa gtccaccctg ttccacgtca tcatcaccaa cctggcgttg tggtgcagcc
    31921 ccgagcaggt ggagttttac ctggtggact tcaagaaagg cgtcgagttc aagtgctacg
    31981 cgacccaccg cctgccccat gcgcgcgtgg tggccatcga gagcgaccgg gagttcggcc
    32041 tgagcgtcct gcagcgggtg gacgacgaat tgaagcgccg cggcgacctg ttccgccagc
    32101 ggggcgtgca ggacatcgcc ggatacctgc ggacgggcgg cccggagccc atcccgcgct
    32161 cgctgctgat cattgacgag ttccaggagt tctttgtgga ggacgaccgc atctcgcaga
    32221 cggcctcgct gctgctggac cggctggtgc ggcaggggcg ggcgtttggc atccacgtgc
    32281 tgctcggctc gcagacgctg ggcggggcct acacggtcgc gcgcaccacg ctcggccaga
    32341 tggtcattcg catcgccctc cagtgcaacg aagccgacgc ctacctgatc atggacgaaa
    32401 acaaccccgc gccgcgcctg ctgtcgcgcc ccggcgaggg catctacaac gacatggccg
    32461 gccggctcga aggcaacagc cccttccagg tggtctggct gcccgaccag gtgcgggaaa
    32521 ccgccctggc cgaagtgcgg gtccgggcgg accaggccgg cgcccagaga cccggcgcgg
    32581 acggagggcc gctttacccg gggccgattg tcttcgaggg caacgcgccg gcggacgtgc
    32641 gggaaaacgc gccgctgcgg cggctgctgg aggccccggc gattgccccg gcggcggcgg
    32701 gccggatctg gctgggagcg cccaattcga tcaaaggccc caccgaggcg gtatttccgc
    32761 gccagagcgg aagccacctg ttgctggtgg ggcagcgcga cgaaacgctg ctgggcctgc
    32821 tttccctcgg gctcctcgcc ctggccggcc agtacccggc gggcgcggcg cggttcatcc
    32881 tctgcgacgg caccgcgccg ggcaccgccc cgcggcagta tatggagcaa atcatccgcg
    32941 ccatcccgca cccggttacg caggcccgcg cgggcgacct ggacgaaatc ttcaaaggcc
    33001 tggcggcgga catgcggcag cgggccgagg cggtggaccc cgaggcggcc ccgccggttt
    33061 tcctgtttct tcacggcctg cagaaatacg gcaagctgcg gtatgaggaa gacttcagct
    33121 tcgccactgg cgatgcggag gccgaggcca atcccgccca gcggctgaac cacctcatct
    33181 gtgaaggcgc gcggctcggc tgccacgtga tcgccacctg cgacacctac aacaacgtca
    33241 cccggttcct cagccggaag gccctcagcg aattcgagct gcgcgtgctc ttccagatga
    33301 gcgccaacga ttccgccagc ctcattgaca gcccgaaagc cagcctgctc gggctgcatc
    33361 gcgcgctctt cttcaacgcg caggagggca ccctcgaaac tttccgcccc tacgcgccgc
    33421 ccgctcgcga gtggctcgaa caaaccgccc gcaacctggc ccgcctgctc gcgccgggaa
    33481 accgcgcgcc cgcgtgaggc gcccgccgcc ccgccgcccc gccgggcggg ggaacatttt
    33541 tcaactcccc cctttacagg gcgatgaagt tggctaaact acccggcaga aaccaaaata
    33601 tatgcgtaac acatttaccc tcctcgccag cctggccatg gccggggctt tgctgaccgg
    33661 gtgcgccaat acggagaaga aactggggcg cggcatgagc aacatgacgg agctcgttcg
    33721 cctgggtgaa atgcgccgca gcatcgagca gaccgccctg ttcgatcaac cgggcggcca
    33781 ctatgccacc ggctttctcc gcggcctcaa tcggacgctc gcccggacgg gcgtgggcct
    33841 ctatgaggtc gtcaccgccc ccttcccgcc ctacgacccg atttttaccg attatctctc
    33901 cccgaacccg gtgtatcccg acaattaccg gccgggcatc ctcgacgatg cgatgttcgc
    33961 caccgatgcc aacatgggct tcagcggtgg cgatatcttc ccctatatcc cgggcagccg
    34021 gttcaagatt ttcgagaccc actaacgtcc gcggctaatc ccctcgccgc cgtcccgggt
    34081 atgcggcccc gcggcgcgcc gccgggcccg gcttcgccgg atcaactttg agtgtctttt
    34141 agcgccggtt tgtggcaaaa gaatgggcgt ggcgctggaa atacaatcgc ccggcaaggt
    34201 taacctgctg ctgaacatcc tcggccgccg cccggacggc ttccacgagc tggaaaccgt
    34261 gttccacccg gtccaggtgt ttgaccatct gcggctggcg cggggcggct ccggcattca
    34321 actgcgctgc agcgatcccc gcctgccgac ggatgcgcgc aacctggtct atcgcgccgc
    34381 ggcgatgttc ctcaaggcgg cggccatccc ggacggcgtg cgcctggacc tccggaaaaa
    34441 tctcccgctg gcggcgggtt tgggcggcgg cagcagcaac gccgccatca ccctgctggg
    34501 cctcaacgag cttttcggcg aacccctcac gcccgaacac ctgcatcgcc tggccgccgc
    34561 cctgggttcc gatgtctcgt tcttcctccg ccgccagccg gccctggccg gcggccgcgg
    34621 cgaacgcctc gaacccttgc ccccttttcc cgccctgcgc ggcgcggcct ttgtgctgat
    34681 ccatcccggc tttggcatct ccaccgcctg ggcctaccag caactggcgc gcttcccgga
    34741 cgcgctcaac ggccgccccg ggcgcgcgcg gcagctcgtc gcgctgctgc aaacggctga
    34801 cctgccaacg gcgggcgcgg ctttctacaa cgcgctcgaa gcgccggccc tggccaaata
    34861 tcccctgctg gccctgttcc aggaattcct ccgcgcccac ggcgcggcgg ccgcgctcat
    34921 gtccggcagc ggctcgacca cgttcgcggt ggtttccggg ctggccgccg cccgggcgct
    34981 ggcggagaag tttaaagccc ggttcggcgc gagcaactgg atcgcggtgc tgccggtctg
    35041 agcctgaccc gcgctcccgc gccggccggc cgcgggcccc tccacgccgc gagccggggc
    35101 tgaacccgcc cgcgcccgcg cccggcccgg cagccgaagc acgcccgcgg gttgcatttg
    35161 ccgggaagat ggagtgtaat cccgccatga taaccaggca tcgctttctt tctggcgccg
    35221 gctggatccg gctgctcttc accgtcctgc cggccctggg cctggcggct tgcgcccgcg
    35281 ccccccgggc ggacgcccct caaaccttcc gggtgatgac ctacaacatt catcacgggg
    35341 ccgggctgga cgagcggatt gatttcgcgc gcatcgccga cctgatccgg cgcgagcagg
    35401 cggacatcgt ggccctgcag gaagtggata aaggcgtgca gcgaacggcg cggcgcgatt
    35461 gtccggccga gctggccgcg ctgaccggcc tgacctgcgt gttcagcaac aacttccatt
    35521 ttcagggcgg cgaatacggc aacgccgtgc tgacgcgctt ccccgtcaag cgctggacca
    35581 accggcatta ccagatgctc cgccccggcg agcagcgcgg ccttttgcag gtggtgctgg
    35641 atgtccgcgg ccgggagctg gtctttctca acacgcatat tgactaccgg ccgggggacg
    35701 acgagcggct cgcgcacgcg ggggaaatcc tggaaacagc ccgcgcctgg cccggacggc
    35761 cggtcatcct gtgcggggat tttaacgaca cccccgggag ccgcacgcac cagaaaattg
    35821 ccaccgcctt cctcgatacc tggctgacgg ccggcacggg cgacggcaac accttccccg
    35881 cgcaaaaacc cgccacgcgc attgactacg tctggctgtc ccgcggcagt gccctcgacc
    35941 cggtcaaggc ctgggtgccc gaatccgacg cctccgatca cctcccggtc gtggtcgagt
    36001 tccgctggcg ttgagccggg ctcaggcggc gcgcagttcc cggagccggg cgaaggcggc
    36061 gtcccacgcg gttgcgtagg gctgcagcgg gctggttttg gtggaatgcc ggaggagttc
    36121 gcgcgcctgt tcgagggagg tgaggtgtcc caatgccagg gcctgcgcca cgacgttgcc
    36181 gagcgcggcg gcatccggcg gcgccgcgac caccggccgg cgcacggcat tggcgatgaa
    36241 gtgctggagc aggtcgccgc ccgcgccgcc cagcaggtag agccgcgcca ccggccgccc
    36301 ggccagggct tccagctctt cgagcgcctg gcggtatgac agcgcgaggc tctccagcac
    36361 gcagcgaatg atcgcgccgg gcttgcgcgg cacgggctgg cgcgtctcgc ggcagaacgc
    36421 ctggaccttg agcggcatgt cgcccgccgc ggcgaagcgg ggatcggcgg ggtcaatcag
    36481 cgcctcgaac gcgggcgagg cgcccgccag atgcgtcagc agcgcgtcat cgatctcccg
    36541 gtcctgggcc ttccagaaac gccggcactc ctcgagcagc cacaggccca tcgtctgctt
    36601 gaaaaaccgg atggaatcgc cgtagccggg ttcatgggtg aacccttgcg cgcgggcggc
    36661 ctccgtgagg atcggctggc cgatcagggc gcccacgtcg gtccgggacc ccagccgcaa
    36721 atacgcccag ctttccccct cgggcacggg caaacccgtc agcgccgccg cggtctggtc
    36781 cgagcaggag gccaccacca cggtgtcttc caggtgggtg gactttgcga tcgccggctg
    36841 cagcggcccc agctccgtgc ccgcggcaac caggggcggc agcagccggg ccggaacccc
    36901 cgccgcctgg agcaggcgcg gcgaccaggc ccgcgtggcc gggttgtaaa gccgcgttgc
    36961 gccggccagc gaccgctcgc cgcgcgccac acccgccagc aggtaattga aggcatccgc
    37021 caccggcaac agccgctcgg cccgggccag ccgccgcggc ttctccgccg ccaactgcga
    37081 aagggtgctc ccgggagaca gctgcccgcc ggtctcggca taaagcgcct cgggcgggat
    37141 ggccgccccc acctttcgca tcccctcctc cccgcggcgg tcgcggtaat gataggtggg
    37201 cgtgatcagg ctgccgtccg gctcgaacag cagataatcg ccgccccagg aatcgcaact
    37261 gatgctgtcc acggcttcct cgtaggtgcc gacggcgttc agcccctgga ggatttcctc
    37321 atacagccac ggaatgttcc acagccaggc gtccttctcc aaaatcggcg tattcgggaa
    37381 actgcgcacc tcgctgatcg tcagcgaatc ctcatgcagc gtgcccagca tcacccggcc
    37441 gctttccgcg cccagatcac aggccacata atacttgctc atctgcggcg gcattgccgc
    37501 aattactgtg ccttgcgcgg cttttccgca atgttcggag ccgggggctg acccgccggg
    37561 gcgcaggcgc ggctggccgg gggcgcaccc cgaaaaagcc cggccgggcg tgtaccgaaa
    37621 aaagacaagg cggcgagcgt cgcgggcagt tcaaagccgc tgatgccgcg tacgccgggt
    37681 tgcgggcaaa ccgcttggcc cggggcttct gagagcgagt gccagcccat gatgacgcgc
    37741 cgcgtttcgc ctgggctttc cgcgctgccc tggcatgacg aagagcaaca ccccgtcgaa
    37801 cggccgcgcc agttcgggac gctgggcgat gcgctgtcag gccacgatcc ggtgctgacc
    37861 cggtcgctgg ccttcgataa gacattcgcg cagccaaaag ccctccaaac gcccgatcgc
    37921 ccttgaaaac cctcctcccg ccggtcttgg atttataact agctgctttt acagaactta
    37981 gcaattatcc ccccgtcttt tctcctcgtt ttggcctttc ccggcattca aagccatcat
    38041 tccgatcccc tggacttacc ctgccatcag gccgtgtaca aggggtgtac aaggggtgcc
    38101 catcgggtgt accagctaaa atcctatgga cctccggtga gcatcccttg tacacggtag
    38161 tgtcctaaaa gttctgcaaa aagcagcggg tcatg
//
LOCUS       DAOPQG010000025        37716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1464_length_37716_cov_16.088633, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000025 DAOPQG010000000
VERSION     DAOPQG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 37716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37716
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1464_length_37716_cov_16.088633"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..908
                     /locus_tag="PKI07_04395"
     CDS             153..908
                     /locus_tag="PKI07_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC50325.1"
                     /translation="MKIVVAGGSGLIGTKLVKFFREQGHSVTPVSRRQGVNAFTGEGL
                     AAALAGAQVVVDVLNLHSPEDQAVMEFFTTTTRNLLAAEAGAGVAHHVLLSVVGVDRV
                     PDNGHYRAKVAQEQLVQAARIPYTIVRATQFFEFIKTIIDLATIGETVRLPAAGMQPI
                     LADEVVAVLADIALQAPRNGIVELAGPELIRQDELARRFLKATHDARNVVTDPRARYF
                     GAVLQERSLVPGPNARLGTARIDDWLRQLPKAS"
     gene            complement(1162..1235)
                     /locus_tag="PKI07_04400"
     tRNA            complement(1162..1235)
                     /locus_tag="PKI07_04400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1201..1203),aa:Gly,seq:ccc)
     gene            complement(1307..1822)
                     /locus_tag="PKI07_04405"
     CDS             complement(1307..1822)
                     /locus_tag="PKI07_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOC50326.1"
                     /translation="MTTPSSEPSTRLLVIGYGNTLRGDDGVGPRAAEAIATLALPGVR
                     ALVCPLLTPELAEAVSQAGVVIFVDAAVDAPREVLMRPLAPAVSSQVVAHAASPATLL
                     ALARDVYGRVPEAWWLTIPVADLGIGETFSPLAQRGLAEAIAKIQKCAAELRPPETPA
                     VAPRAPRTPPQ"
     gene            complement(2365..3225)
                     /locus_tag="PKI07_04410"
     CDS             complement(2365..3225)
                     /locus_tag="PKI07_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC50327.1"
                     /translation="MLLLSCAAEPPPGRPGEYQLFARTNLAAWCIVPFDARARGPEER
                     AAMLERLGFKLFAYDYRAQHIPTFDAEMEALKRHGIRLLAWWFPWELNDEARLILEVL
                     QRHQLRDVQLWVSGGGQPTRDPAEQQACVEAEAARIRAIALAASRISAQVALYNHGAW
                     FGEPENQIAIIEYLRTQGVTNVGIVYNLHHGHDHLDRFPELLQKMKPHLLALNLNGMT
                     RDGEKRGRKIIPLGQGDLDLKLLRIIRDSGWRGPLGLLNHTDEDAQTRLQDNLSGLDQ
                     LVAQLEGRGK"
     gene            complement(3360..4295)
                     /locus_tag="PKI07_04415"
     CDS             complement(3360..4295)
                     /locus_tag="PKI07_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50328.1"
                     /translation="MKIMDSPRTGKLASVVFYPSRFGQCARTRTIPRDPKTERQNRMR
                     AIFGSASRSWGVALSEDQRLRWVAAAQAVPSHPSLAQYSYLSGQQFHVQINSTLQCIG
                     LPPVSEPPAPVTFGPNPVSDLVIDHDPEKGTRLRLNIGPASEDIMVFGQAPCSAGRMK
                     PRRVYYLGLLCPAADSQRDITDLYTARFGRPAPGQKVFIATCQTKDGWKARESVFSAI
                     VPPAPSVEKRQVPLKAIVTTPAPAAKPQPSASAAKVSPPLHQAMYKGSTRDVPRENKA
                     QKYGHLTSIPGTPLVHSVWAALRRLLLPGMREVEA"
     gene            complement(4575..5555)
                     /locus_tag="PKI07_04420"
     CDS             complement(4575..5555)
                     /locus_tag="PKI07_04420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC50329.1"
                     /translation="MPTPTVAVIIPTYNRWPLVARAIESVLAQSHAATECVVVDDGST
                     DGTPGHVRAQFGERVRRIVLPQNGEKSAARNAGIRAATAEYVCMLDSDDELPRHSVRD
                     RLGVFLRDPAFNGVAYGFTSLGTDADEARRAGQAAPEGDVLLAYIRHPFVHNNAFLLS
                     RPNMLQHGMYHERLTHREDKELLIRLAASLDFRCCGTLVNRFHAVRGSARMNYRKAAA
                     QGLLMLESLRANPRVRERLGANFRLLEQAEVWEHLRDLYKVGRFADYRAACARSWALF
                     AGRGKLRRRLLRRWIGSWVLQALAVGQADRVPQNPAAAGALAPPSKPDRE"
     gene            complement(5571..6830)
                     /locus_tag="PKI07_04425"
     CDS             complement(5571..6830)
                     /locus_tag="PKI07_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOC50330.1"
                     /translation="MSGLVHVVCATPPTPNPGMGSVDLAFYGLWQRHFPDVDVRFCQL
                     FTADEQHRHRSPRQQQEFAAREALPFAYHSCRNRLPALAAGRAVIFWGDFLHMAHYRQ
                     QLARRLVALGITPTLAEGIAAVDQHFFLADSPDGMLNQTLAFGGTLIFNCAADRLDPA
                     YENLLRRWARQTRGIWMRDLFSARLINRLRNDWSQSFLGVDCALLLEDASLAGLPRQW
                     AADPRAGTDCGVAGLFLSRNVVPQRLMLAFARAVCRARGVRAEWLPWRSSRFPEWPSR
                     WAFPELARPVSTAPPALVDLLDRIRRYDFVISDTYHVCVNAWRLGIPAICIGETWSRR
                     EMDVSCGVVGAWRDKRWVFYAMNEALEFYIHAGELTRRRWRERRAAETAERLASRPLL
                     DLVRHGIQERAAAARQAFLDALFSLIR"
     gene            complement(6884..8479)
                     /locus_tag="PKI07_04430"
     CDS             complement(6884..8479)
                     /locus_tag="PKI07_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50331.1"
                     /translation="MRQTWAVKAGKTSHQSGSPGVSRRKFIVTGGTAAAAFTIVPRRV
                     LGGAGFVAPSEKITLALIGCGTQGLRELPSLFALPEVQIVAVCDPVKDGHDYLDWSRD
                     GHRAAIAEMLRQPDWRRGQPGIPGGREVAREVVETVYARQRSEEKFRGCTAYADFREL
                     LAQERDVNAVKVMTPDHLHAAVAIAAMKQGKHVMMHKPLANRLLEARWVIEWARKTKV
                     ATHFLAASDGADIQEIKRWIDGGAIGSLREIHNWSNRPVWPQYSAIPTDQPPVPAGFD
                     WPLWLGPSRDRPYHPCYTHAVFRGWYEFGGGALADMGHYSLWPVFQLFGLEAPVRVES
                     CPTHVCTVRDQVAVRVRNDYSFPAACTIRFKFAARGARPALDLFWYDGSLRPPTPEEL
                     EHDNQALPSEGMMFVGDRGKILAGFRGEAPKIIPEKKMREFRAAASARETAEAQTRPP
                     TRRDAAAAWVAACKGGPATVGDFLLAGPISDAFNLGAVSLRLGGRRLAFDAAAAKIGA
                     DAEANQCLAREYRAGWELAGPAA"
     gene            complement(8734..9135)
                     /locus_tag="PKI07_04435"
     CDS             complement(8734..9135)
                     /locus_tag="PKI07_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50332.1"
                     /translation="MDSFDNKPSSPAEANPLQAQYDALQHMVVSILILVIVISGTLNI
                     YFLRQWRTVSKDLAAVSPQATKMIADYQKTSAPAMNDFVKKIIEFGRTHPDFAPVLTK
                     YNLQVDTSTGATVSPPASPAPTAPAPAPKKK"
     gene            complement(9142..9426)
                     /locus_tag="PKI07_04440"
     CDS             complement(9142..9426)
                     /locus_tag="PKI07_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50333.1"
                     /translation="MKNNPLPRVLLGVLLVSALASVVLCWLYISNTRQLRTLQGQLNI
                     INNNRTVMATLANDALEYSKKNPAIDPILESVGLKPAKTGTPAATKPATK"
     gene            9618..9836
                     /gene="infA"
                     /locus_tag="PKI07_04445"
     CDS             9618..9836
                     /gene="infA"
                     /locus_tag="PKI07_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOC50334.1"
                     /translation="MAKEEPIELSGSVTQVLPGTMFRVALPNGHEVLAHISGKMRKNF
                     IRISVGDKVNVEMSPYDLGKARITFRHS"
     gene            9938..11332
                     /locus_tag="PKI07_04450"
     CDS             9938..11332
                     /locus_tag="PKI07_04450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC50335.1"
                     /translation="MRISFFGATRTTTGSLYLLELNGQRLLLECGLFQGRRGESIERN
                     RTFPFDPKAINAVVLSHAHVDHCGNLPNLCRQGFEGNIYCTFATRDLASIMLEDSAQI
                     QRDDAAFVSRKRAKRGLPPVEPLYTALDADRAVRQFVSVNYNRPFPVSDGVTVTFHDA
                     GHILGAAQVAFDIRENGRQYRYLFSGDIGRGNDDILRDPERVEDVDYLQVESTYGARE
                     HAPKPRANADVERMVLETLGRQGKVIIPSFSVGRTQQVVYTLHQLTLAGKLPRVPIFV
                     DSPLSVNATEVYRLHPECFNEATYKFLREKQNPFGMENLTYIREVAHSMKLNDLKEAA
                     IIISASGMAEAGRIRHHLANHIGNPVNLILFVGYCAEHTLGAQILAGRNPVNIFGEPH
                     EVRARIASIDSFSGHADKHELRRYVENITGEIKQISVIHGEEEQALAFGETLRQMKPQ
                     AQVLVPEYRQVQEI"
     gene            11591..12709
                     /locus_tag="PKI07_04455"
     CDS             11591..12709
                     /locus_tag="PKI07_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50336.1"
                     /translation="MSANFSTGTPDSVPAPLRRELSFALIGCGRVSENHLAALTAAEV
                     PARLVAVADVDPSRAQQKGAKYGVPAYTDFSVMLAEHPEIEVVDIATPTGYHSAHVQA
                     VARFQKHIVVEKPMALTLEDCDAMLAACRQNGRRLFVVKQNRFNPAVQAARRAFTAGR
                     FGNLVLVTVRVRWRRDQSYYEADGWHGRWALDGGVMSQQASHHIDLLQWFLGPIESVQ
                     CQAANRLLRLEAEDTAVAIFKAASGALGILEATVAARPRNLEGSLSILGEKGTVVIGG
                     VAVNQILCWEFTEPQPGDEVIHQQASQAVPNVYGNGHVPYLRDVAEAILDNRPGLVEG
                     NEGRKNVEILTALYESAAGDGAKLSPGCPIVRSRLGRG"
     gene            12754..13911
                     /locus_tag="PKI07_04460"
     CDS             12754..13911
                     /locus_tag="PKI07_04460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC50337.1"
                     /translation="MNILHLFSDWKWTGPSEPVLNMCRGLQQLGHAVTLACPPRAGRE
                     SRSIATFARDRGVNLSHQFELNHHQVLRTSLPDLQRLTRFLRHHPLDIVHTHLSHDHL
                     LGGLAARLSARPVVVIRTDHYRDSFAPGFLNRWLLTRLADGVVTFSDRAQLRLRQQFG
                     LAAERVARISPALGPEAFTPVPETARRQMREEFGFRDDEVVLGVVARFQKYRRMELFL
                     AALAQASRTAPQLKALLVGRGGDMQQTVLDPIRQFGLEPRVKVAGYLTDRYRAALASM
                     DGMVFLIAGSDGTARAMREAMALGRPIIANNLGMLPELNESGKTGLIFNDTADDLARC
                     MVQLASDPAQRRRLGEAAALKAAKEFRLDAQAVALADFYSRRQRSRVLSAA"
     gene            14017..15873
                     /locus_tag="PKI07_04465"
     CDS             14017..15873
                     /locus_tag="PKI07_04465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC50338.1"
                     /translation="MSETQADRLPPPPAPGRRRAKAAQIATLFIPVTVLLFFVIRTTG
                     YPLLSRHDVRVGSYVLDAVQNGNWIIQKDYGGEIAAKPPLLTWCAAVTTLLAGRISRV
                     AIHLPAAVALCATALALLAGGRSRFGERAGFLAAMIYVVSPAGYSQLATARYDGLLAF
                     MVVLSAFAAFRAWHCGSGWIWFWLAAAAGTLVKGPLAVLLGGAGLLAYFWEWRTGSRL
                     PLRGNHFPGLLLFLLICGGWFLLAYAQLGQPLINKMIGRELIGAAVGAEDGPSPWGPL
                     KPPRDFLITFAPWSFACLYSFWRIWKRPDADPTRRRFERFLASSLLAGIIVFSAGGHR
                     QSRLIVPLLPLAALLAGRELATLLALWSDRKLFRLAAVLAVGMGIGIFIHGHFLMRRS
                     PRVTETSAARQTARLIREQLGPEAPLTYVDSPFAVQFYLNDVRPAASFERAAALLRGG
                     AGALVVVRDVDRLRKQMGPDADRLHEILRLPPTGEPWIQVLGNQPQPLTNQPVALLLG
                     PLRVELDSLRLQRLRINYRHGIDLFVHGNPAPGKLRVENQSASPQWVRLRLIEAKSGR
                     VITETKRSLAPGTVWDFPQDRPLALGKGAGPFTLARGPDSRPPAPAISPAGP"
     gene            complement(15809..16660)
                     /locus_tag="PKI07_04470"
     CDS             complement(15809..16660)
                     /locus_tag="PKI07_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50339.1"
                     /translation="MKRSRPEPPTGAEVLQAGGRGQTGNLLYFFPSEQPPLVLKVYCF
                     RRSRWREFWKDVSERVFEGKRGATAATRCATERLILDLWAREGFDVVRLVARPLPSGM
                     EKPALWLEYCAAPVLAKVLADPNRPPGEKLAWVELLGQALSRRHRRALELNDPRLVHE
                     HGQIKHFFAQDKRLIAFDLEHGYRSGYPVVRAIGRELTGIADSLLRADGGGGEEFLRA
                     LMRGYANPPLVRAAAREATQRGGLAGIIRRLLGYLRGGKTDKTQVMARLVRLLEPEAG
                     SPARARG"
     gene            complement(16703..17554)
                     /locus_tag="PKI07_04475"
     CDS             complement(16703..17554)
                     /locus_tag="PKI07_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50340.1"
                     /translation="MSQSNEVIRLPDLKPYLLLQNGNYLYKIPFREGWAVLKVYYGSR
                     SWFSCLAKSFENVVLAGQTSYLPRTRRRVEAECMAIWRKHGFRVFGTYPEVRVEAPGC
                     PEGGYMLFEYVEAPKLGDWMKDESRSVEERLAVYRAWLLEWSRRHDIAVREREPRLAH
                     ENGDGKHVMLLPGGEFLWFDFEMTFRSRRRVAEHVSHEIIQYLWFLWKSVPPGIRERL
                     IPETVRHYPQKERLGRAYEYFWRHPNLLHRFGRACERAFKAKARSPTSKYSVARLLRE
                     ELERAKA"
     gene            complement(17760..18836)
                     /locus_tag="PKI07_04480"
     CDS             complement(17760..18836)
                     /locus_tag="PKI07_04480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC50341.1"
                     /translation="MNILHLNNEKSWRGGERQTLLLAAGLRACGIGSVIACRSDGPLA
                     RKALEAGVPVRAIPGNNLGAGLALPRLAREFDLIHCHTGRGHSLAALTAPGYRKPFLA
                     TRRVDFLPRRGWFNRYKFQRAAQVVCISQFIANQLAAWGVAPERMCVIRSAVPMPAPG
                     LLTPVHRAAVRTRLGIGPGTKLVGNIAALVGHKDQATLLRAAKGLIARRRDVRFVILG
                     DGELRGALLAQRRELELEEAVLMPGFIPQAEQWLAAFDVFVISSCMEGLGSIVLDAFA
                     AGVPVAATAGGGLPELVRDGETGLLVPVGDHAGLAEAMLRLLEEAELAERLRTAARDW
                     VQRERSVEAMVQAYLQLYARLLSR"
     gene            18998..19828
                     /locus_tag="PKI07_04485"
     CDS             18998..19828
                     /locus_tag="PKI07_04485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC50342.1"
                     /translation="MQSRLPLSVAVITLNEEVNLPRCLGSIRDLAAELVIVDSGSTDR
                     TQEISRQMGALFIAQPWLGFTRQKNFALERCSQPWALCLDADEAVSPELAEAIRHAFA
                     SPPSVNGFELNRRTSYLGKWIWHAWYPEWCLRLVRQEAARWAGADPHPSLSVTGATAK
                     LSGDLLHYSYGDLREHLERTLRYARTSADAMALAGRHCCWYHLVISPWLALGKRLILK
                     QGFRDGWRGWIIAYSSFLSVFAKYAFLAEQQRLAQRRPQPPTDLPTGTSSASKPSGPA
                     "
     gene            complement(19858..21738)
                     /locus_tag="PKI07_04490"
     CDS             complement(19858..21738)
                     /locus_tag="PKI07_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC50343.1"
                     /translation="MNHILKVLRFGLPYLRRYWIRLAAGVLFGILFGATSATFVWGTK
                     TLIGRMAPETEARAHLKKEKPGREQKLDRIKAELDRRTREWVDPWLPYAGRPVDWRQV
                     LGGLLLFPALVAVRGIMAYLSAYCMAWVGEQVVNDLRTDVLIRLSTLSLDYFNRATMG
                     DMVTHVNGDTATLQRCLRVGCADLVQQPMTAIGVLVALCVIDWRLTLAAMIFFPACVI
                     PVVVMGRRARLAARASTKVGVTQASLLYEILSGIRVVKAFGLEAMQVERFRRFSRQLV
                     RQNLKGVRAKELVNPIIETVSVMGFGLLVVYITYQNRPVSDMVGFLTGLIFFYTPVKK
                     VAQLHVMFEQTAFGVQRLLQIMTERPTVKEAAAPRRLLEFKSSVVFDQVSFSYGREPV
                     LRELSLEIPRGTRLGVAGESGSGKSTLVNLLFRFFDPVQGVIRIDGVDLRQIANADLR
                     QLMALVSQDIVLFDMTVAENIALGREGATREQIEAAARAAFVHEFILSLPQGYETRIG
                     ERGVTLSGGQRQRLCIARAFIRNAPILVLDEATASLDSKAEAEVQAAIDRLAEHRTVI
                     AVAHRLSTLAAMDRVIVLAEGRIVESGGFDELLLRGGVFASMAARQGILPSGGSAEIV
                     ET"
     gene            21934..22026
                     /locus_tag="PKI07_04495"
     tRNA            21934..22026
                     /locus_tag="PKI07_04495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21968..21970,aa:Ser,seq:gct)
     gene            complement(22066..24852)
                     /gene="mutS"
                     /locus_tag="PKI07_04500"
     CDS             complement(22066..24852)
                     /gene="mutS"
                     /locus_tag="PKI07_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOC50344.1"
                     /translation="MASNAQLTPMMAQYRRIKGELPKDALLMFRLGDFYEMFFEDAQT
                     GAQLLNVALTKRGVVPMCGIPFHAASAYIGRLLRAGRKVAICDQVEEARPGQLVKREV
                     TQILSPGTHFDERVLKAERNNFLGAVYAVGRNYGLAFADLTTGDFMATEVAGEAEVLT
                     ELQRLRPAELIYPAEAGAVRDLLQGANRPGKPGDPASPAFPAGPAKSGLARTPAPPAK
                     AGSPGAEGVGYADVAPTGFGWILNGYDGWVFAPETALFTVREHFKVASLDGFGLKDRT
                     AAIGAAGAVLHYLTQHLRRDAASLTRISFYQRSDYLALDFSTLRHLEVLEPLHPDTPR
                     NASLYGALNRTVTPMGARRLRDWLSQPLAKVGPIQRRQEAVQTLVNHSAALEELRAQL
                     AQVRDLERTLGRLSAGTGNARDLATLRAALERLPDLKRLVRELTTGNAAEEGRRSERE
                     LCLLAPAAPGEQAPPPVQTLSEGEVHVGGHPLLLEDLGGQVTELPDLAELIARAIVDE
                     PPLAIKEGGLIRDGFDPALDELRAAMREGKDWIAKLQQDEIVRTGIPSLKVRFNSVFG
                     YYLEVTKANLDKVPPDYIRKQTIANGERFITPDLKEMEGRILGAEERGVKLEYDLFQR
                     VREAVLERLRELQQTAAALAQLDVLGAFAETARLHNYCRPQLGEASGLYIRDGRHPVL
                     EQSLTEERFVPNDTFLGSETQIALITGPNMAGKSTYIRQVALLVLLAQTGSFIPAAEA
                     RVGLVDRIFTRIGASDDLARGQSTFMVEMCETANILNNATPRSLIILDEIGRGTSTFD
                     GLSLAWSIVEHLHNTVGAQTLFATHYHELTELAGRLPRLKNFNVAVREWHEQIIFLRK
                     IVAGGTDKSYGIQVARLAGVPRSVLERAKQILVNLEESELTPEGNVRQATRRQQDRDR
                     LKQLAPPPQMDLFG"
     gene            complement(25132..25638)
                     /gene="ribH"
                     /locus_tag="PKI07_04505"
     CDS             complement(25132..25638)
                     /gene="ribH"
                     /locus_tag="PKI07_04505"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOC50345.1"
                     /translation="MLKTIKKKKLQASGGCFAIVASRYNGRYVDSMMRAAVSELKRAG
                     AKQIRIVRVPGAYEIPLVAARLARNCSPHAQSLTSSGIRPAAIICLGVILRGETVHAA
                     HIGEAVSHALMEVQVAYEVPVIHEVLLLENEAQARARCLDPQRNRGREAAQTALTMAQ
                     VMAGLRAK"
     gene            complement(25669..26868)
                     /locus_tag="PKI07_04510"
     CDS             complement(25669..26868)
                     /locus_tag="PKI07_04510"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOC50346.1"
                     /translation="MAQQFNSIEAVMADLRRGKMVIVTDDADRENEGDVIAAAQFVTP
                     AVVNFMARHGRGLICVPTTSERLQQLGIERMVRQNRENFKTDFQVSVDAARGITSGIS
                     AADRARTIRVLADPTAVPEDLVQPGHVFPLRARPGGVLQRAGHTEAAVDLVQLAGCRP
                     IGVICEIMNDDGTMARLPELMKFARKHRLKVCAIADLIQYRRAREKLVERVEVVKLPT
                     EYGKFDLYLYRSKLEGLNHLALVCGNVAGKRDVLVRVHSECLTGDVFSSCRCDCGPQL
                     RQAMRQVAEAGCGVVVYMRQEGRGIGLAPKIKAYKLQERGYDTVEANQKLGYAMDLRE
                     YGLGAQILADLGLKRIRLLTNNPRKIVGLEGYGLKVIERVPIQVKPNPHNARYLKTKR
                     EKLGHLL"
     gene            complement(27045..29174)
                     /locus_tag="PKI07_04515"
     CDS             complement(27045..29174)
                     /locus_tag="PKI07_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOC50347.1"
                     /translation="MNRFAKHRQNSGAALGTLLALLVLSSSRGAVGDFTFIHTSDLHA
                     PMAQSKTTVAQIVGLGPIDLAPYGLEIPKPSFVLATGDLNEFGGGSGWWEEYQAYWKG
                     ITIPVFPQLGNHDNTWRSNLKSLRDAGLAACYSFDSHTCHFVGLMTPTLQDPRPSVGE
                     EQLLWLKQDLAKVGPQRPVFVFFHHPLPGSEFASRYDYDRLLDVLHGYNTVLMMAGHS
                     HGYAHRLVEGIDQTTGGSTFGPNAGFAVVSVKEGMLRVAYWKADAPAPGLKLLEKPIP
                     EQTSYPKIEIISPAFRQAGGSSVSVSARLSGAVALEKATYSVDDEIKGDLKLSGTAPN
                     WTADGTVEVSRLLPGAHYLRVEFMQGSERFSRSTQFFFEPEQQPTAWRAYLGASSKVT
                     PAIVDEVVYVGANDGALRAFNARTGAALWEVRTGAEILAEPLIAKGTVYTANGLGIVS
                     AYTTSGEKLWSFTAGDAVYSSPVLADGKIIFGCNNGWLYALDAGTGKLAWTNKDATYA
                     IESKPFVWNGKVFFGAWDQYVRCVNARDGKLVWQQLGEGSRVVKGAKRYYSPADAMPV
                     VAEGKVMIADRNMMLSILDAETGEPAGSMTGVSATGISEDGQHVYLRKTNGELAKVDS
                     SGKEIWRVAALLGAIPTAPTEKNGVVYVCSGKGVVSAIAADNGKILWQYQASPQLYVM
                     GSVVSDGTHAYLTSFDGSLTAIKCPAQ"
     gene            complement(29384..30577)
                     /locus_tag="PKI07_04520"
     CDS             complement(29384..30577)
                     /locus_tag="PKI07_04520"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HOC50348.1"
                     /translation="MGSEYSTAAHWPHEFSEPGLQKWAENLRRQLAAPRVSLGLVFMS
                     PRFFDRAQQVLEILRVHAHIPLLAGCSSQSLIVGDHEVEKDAGLTLGLYALPGADLRA
                     VHFSQEQVEEANGPGYWHLETGVEPDRSNGWLTFINPFHLDSETWLRTWNEAYAPLPV
                     LGGLASGESDAQATQVYLNGEVYPEGGVAISVGGAVRVVGVTSQACTPIGQTWTLTKV
                     EQNIIHRIGNRPAYEVLAETFNQLPSDQQETARGNLFIGLVVNEYLEDFQRGDFLIRN
                     LLGADPQSGSIAVGALPRLGQTIQFQRRSAATADEDMRELLARAKQQLGDTPIYGGCL
                     CSCNGRGQSLFGQPNHDAQMVQQRFGPLGLAGFFCNGEIGPVGQKSFLHGHTASLALF
                     VKKTD"
     gene            complement(30864..32087)
                     /gene="rlmN"
                     /locus_tag="PKI07_04525"
     CDS             complement(30864..32087)
                     /gene="rlmN"
                     /locus_tag="PKI07_04525"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOC50349.1"
                     /translation="MLTDIKSQTAEELENQFRAWGQPAYRVQQLLEWLYAHRATSWEA
                     MTNLPRALREELRRHYSLAVLELVRKQGARDATQKFLWRLSDGALIESVLMAANPALY
                     GEPSDRHTLCVSTQVGCAYGCKFCASGLAGWKRNLRVEEIVEQVLAVERWNAGESASG
                     AACENPGFGNPGPEAGGENGRAQDAPGARPGASFVPHGAPFASPARLVSNLVIMGMGE
                     PLANFDNLLKALRILNAPWGGGIGARKITISTSGLAPQIRKLAEEPQQFRLAISLHGA
                     TDETRSRIMPVNRKYPLRELTAACEEYQRKRGRMITFEYILIAGVNDGLDQVLPLAAL
                     ARRFKAKVNLIPYNRVEGLAWERPAEDVQEAFLQALAKQKVKATLRREKGGDIDAACG
                     QLRLKTERELGRRAS"
     gene            complement(32190..34370)
                     /gene="glgP"
                     /locus_tag="PKI07_04530"
     CDS             complement(32190..34370)
                     /gene="glgP"
                     /locus_tag="PKI07_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOC50350.1"
                     /translation="MTKKPLTTQELQELTVALNRVARNLWWTWNQEAQDVFQELSPRG
                     WQNLYHNAVAILREVSDYELRVRLQDPDFCDRVNEVLRLFETYMNDSRTWAHEHAPAL
                     RANPVAYFSAEFGFHEALPIAAGGLGILAGDHTKSASDLGVGFVGISLFYREGYFQQA
                     IDTNNWQTEYYTQLKPKNLPIEPVLNEKGEPVVCRVGIGMNDVAFQAWRVNVGRATVY
                     LLDANRPENEQHFSELTQRVYGGDSTTRIMQEMLLGIGGVRLLRALGVKPSVYHLNEG
                     HAAFLTLELMRERLAAGRSFAEALSQTRAECIFTTHTPVEAGHDRFRPDLVDYAMQRY
                     RAELPVPFSEVLKLGRVNPQDANEPFCMTVLALKMSRAANAVSELHGRVSRQMWHCLY
                     PDRSVDEVPIGHITNGVHLLGWMKGTVRRFWQRKLKASLADAAPPGFRGPGADWAAQV
                     NSPEFWQRVADPEFISDEELWALRYKLRRELIEFARRRLLIQQQRLMHGDFIAFDQLL
                     NPDALTIGFARRFATYKRAPLVFQQLENIVRLTRDPERPIQFIFAGKAHPRDDEGKRY
                     IQYVIHLSKYSDLKGHLVFLENYDIHVARQMVSGCDVWLNTPRRPLEASGTSGMKSGT
                     HGSLNLSILDGWWREGYDGSNGFAIGEDAHASSVEEQDKVDSANLYKVLTQDVIPLFF
                     QRDAQGIPRQWIQRIRRAVVTLVPQFTTARMVREYTEKYYLPKT"
     gene            34632..34919
                     /locus_tag="PKI07_04535"
     CDS             34632..34919
                     /locus_tag="PKI07_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOC50351.1"
                     /translation="MKRQSKSEFRCQQECRCEVCPLSQIKAGVAVRIKQLCGAPELQN
                     RLRELGLGEDRIIKLLTSQTNFICQVCNARLAISQQLAQLILVEPLRPAVV"
     gene            complement(35293..35439)
                     /locus_tag="PKI07_04540"
     CDS             complement(35293..35439)
                     /locus_tag="PKI07_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50352.1"
                     /translation="MRVIRHSEVPQELEAAALWYEERQPKLGSDFLEEYRATLGRILA
                     EPER"
     gene            complement(35417..35662)
                     /locus_tag="PKI07_04545"
     CDS             complement(35417..35662)
                     /locus_tag="PKI07_04545"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HOC50353.1"
                     /translation="MSIAAEKVAEVLALPGQDRAYRARQLIASLDDTIDAGAETQWNE
                     VIDRRSREMAEGRVDARPEEEMIRDIQAKLHARHPAF"
     gene            36479..37072
                     /locus_tag="PKI07_04550"
     CDS             36479..37072
                     /locus_tag="PKI07_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50354.1"
                     /translation="MTHLNLPRFQYTVREPVSGACFTGYADERSKSYATLLAEQLAVP
                     LAGHGVDLSGVVGQTDNGSEFLENQPAQGLPTAECALGCEHRYIPPQRYPWQSDVETV
                     PRRVEDECFDRETFRSPTDFWAKVITYWLYFNLGHPNRGKEWQSPLQILQRQAPALVG
                     AVLDWRPLDLAKRHHFYLPKPLYQGHDVPRFPFFLLF"
     gene            37140..37445
                     /locus_tag="PKI07_04555"
     CDS             37140..37445
                     /locus_tag="PKI07_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOC50355.1"
                     /translation="MTLTKRDLVLGICEETKLPYHQVSNVVQLTFDHIINALAAGRKV
                     ELRNFGIFEVRLRKARIGRNPAAPESQVPIPERYGVKFKAGREMRLSVRKIAPKSNA"
BASE COUNT         6955 a        12673 c        11181 g         6907 t
ORIGIN      
        1 agcttaaccg ccaggccatt tattcgggaa gcaagccggg cggcgggcag gattggcgaa
       61 ccgaatacaa cgcgttagat acaggaactg gcgcgcggag atggaggttt aaactggcgg
      121 gcgagaactt atggcattat aatagtggca gcatgaaaat tgttgttgct ggtggaagcg
      181 gattgatcgg aaccaaattg gtcaagtttt tcagggagca aggccattcc gtaacacctg
      241 tctcgcgcag gcagggagtc aacgccttca ccggggaggg gctggcggcg gcgctggccg
      301 gagcgcaagt ggtggtggac gtgctgaatt tgcactcgcc cgaggaccag gcggtgatgg
      361 aattttttac caccaccacc cgcaacctcc tggccgcgga agccggcgcc ggcgttgcgc
      421 atcacgtgct gctctcggtc gtcggcgtgg atcgcgtgcc ggacaacggc cattatcggg
      481 cgaaggtggc gcaagaacag ctcgtccagg ccgcgcgaat tccgtacacc atcgtccgcg
      541 caacgcagtt ctttgagttc atcaagacga ttattgatct ggccacgatc ggggaaacgg
      601 tccggctgcc cgccgcgggg atgcaaccca tcctcgcgga cgaagtggtt gccgtgctgg
      661 ccgacatagc attgcaagcg ccccggaacg gcatcgtgga gttggccggc ccggaactga
      721 tccggcagga cgagttggcg cgtcgttttt tgaaagcgac gcatgacgcc cgaaacgtgg
      781 tgaccgaccc ccgcgcccgc tatttcgggg ccgtgctgca ggaacgcagc ctggtgcccg
      841 ggccaaacgc gcgcctgggg acggcgcgca tagacgattg gctccggcag ttgccgaagg
      901 cttcataatc gccgggcggt attccctcgc tgcgccccgg agaggccgtc agataacgcc
      961 caacgaacaa tggggccgaa gcaccctgcc cctttgaggc gcacaactcc ctccgctcgg
     1021 gctcgtacca caacgcccga aagcaactct acgtaacggc atggaccgcc cggtgtcctt
     1081 ttatgcgcca atcatgcgtt ttgagtgcga gtcggggcga aaaagcggga aaatctgaaa
     1141 ctttcaagga ggacgtggaa ttggagcggg tgaagggaat cgaaccctca tctcaggctt
     1201 gggaagcccg cattctacca ctgaaccaca cccgcttcag tgaccaggct catttgtagc
     1261 ataacccgcc ggggctggca agggttgttt gcgtcttgct ggcgccttat tgcggcggag
     1321 tccgcggggc gcggggggcg acggcgggcg tttccggcgg gcgaagttcg gcggcgcatt
     1381 tttggatttt tgcgatggcc tccgccaggc cgcgctgcgc cagcggcgag aacgtttcgc
     1441 cgatgccaag gtccgcgacg ggaatggtca gccaccaggc ttcgggcacg cggccatata
     1501 cgtcccgagc cagcgccagc aaggttgccg ggctcgccgc gtgcgccacg acttgcgaac
     1561 tgaccgctgg tgctaagggg cgcatcagca cctcgcgcgg cgcatccacg gccgcatcca
     1621 caaagatcac cacgcccgcc tgtgaaaccg cttccgccaa ctccggcgtc aacagcgggc
     1681 aaaccaaggc gcgcactccg ggaagggcca gtgtggcgat ggcttcagcc gcccgagggc
     1741 ccaccccgtc gtccccgcgc agcgtgttgc cgtagccgat gaccagcagg cgcgttgagg
     1801 gttccgatga tggggttgtc atatttcgct ttgagaggat tggagaaccg ctggctggcg
     1861 aaaattgccg gttggcgatt ccgcaaagcc cagccaccag gcaatgctcc gctccggggg
     1921 ccagccaatg gctgaaccgg gcggcaactg cgacaggaag agttgctgaa ccgcggcaac
     1981 accggttgtc cgggcggggc ggaacaacct ggccgccgcg ggagaacggg cggacagatc
     2041 gcttgcgttc tccgtcacca ctggtgcagc cggccgaatc atcgcgcctc tcccagccgg
     2101 gctttttccc gtggcggctc tgccgggtca aaacgaagga cgcgcccgcc ccccgacgcc
     2161 cagccgggtt caaaccgggc atacgcaggc gcaacagcct tcatcgccgc ccccggcttt
     2221 ccgtctcggc aaagcagaag ggtccttctc cgttccgcga ccgccgccgc caacttcgca
     2281 agcgggttcc cggactggtg cgcatggctc attctcatag tccgggccca gtctgggcca
     2341 tcccatcgcc ctgtcaaccg gaagctattt tccccggcct tcgagctgcg cgaccaattg
     2401 gtcaagtcca ctgagattat cctgcaggcg tgtttgagcg tcttcgtcgg tgtggttgag
     2461 aaggcccagc gggccccgcc agccactgtc gcggatgatg cgcagcagct tcaaatccag
     2521 gtccccctgc cccagcggga tgatcttccg gccgcgcttc tctccgtcgc gggtcatgcc
     2581 gttgaggttg agcgcgagca gatgcggttt catcttctgg agcagctcgg ggaaccggtc
     2641 gagatggtca tggccgtggt gcaggttata gacgatgccg acgttggtga cgccctgcgt
     2701 tcggagatat tcgataatag cgatctggtt ctccggttcg ccaaaccagg cgccgtggtt
     2761 gtagagcgcg acctgggcgc ttatccggct ggcggcgagg gcgatggccc ggatgcgggc
     2821 agcctcggct tcgacgcacg cctgctgctc ggccggatcc cgggtcggct ggcctccgcc
     2881 gctgacccag agttggacgt cgcgcaactg gtggcgctgg agcacctcca aaatcaaccg
     2941 cgcttcgtcg ttcagctccc atggaaacca ccaggccagc aaccggatgc catgacgttt
     3001 gagcgcctcc atttcggcat cgaacgtcgg aatgtgctgc gcgcgataat cgtaggcgaa
     3061 cagcttgaac cccaaccgct cgagcatcgc cgcccgttct tccggtccgc gcgcccgggc
     3121 gtcgaacggc acaatgcacc aggccgccag gttcgtccgg gcaaagagct gatactcgcc
     3181 cggccgcccc gggggcggct cggcggcgca ggacagcagc agcaccagta ccccgctccc
     3241 cagccaaagg cggagaagcg cgccccatgc cgctttcctt cgtcgcctcc cgggagccag
     3301 acctttcaat ctctcatggc gctcttttcg ctcactcagg gcaccgttgg cgttctgcat
     3361 caggcttcaa cctccctcat tcccggcagc aatagccgcc gcagtgctgc ccatacgctg
     3421 tgtacaaggg gtgtaccagg gatgctcgtc agatgcccat acttttgcgc cttgttctcc
     3481 cggggtacat cccgtgtact ccccttgtac atggcctgat gcaaaggggg agaaaccttg
     3541 gcggcagagg ccgagggctg gggcttggcg gcgggagccg gtgttgtaac tatcgctttt
     3601 aaaggtactt gccgcttttc aacggagggc gccggcggga cgatagcgct gaacacgctt
     3661 tcgcgggctt tccagccgtc tttagtttgg caggtggcga taaaaacctt ctgcccgggg
     3721 gcgggacgcc caaaccgggc ggtatacagg tctgtaatgt cgcgctggct gtcggccgcc
     3781 gggcacagca ggccgaggta atagactcga cggggcttca tccgaccggc gctgcacggc
     3841 gcctgtccaa ataccataat gtcttcgctg gccgggccaa tgttcaaccg caggcgcgtt
     3901 cccttttccg ggtcgtggtc aattaccagg tcgctgacag ggttggggcc gaaggtgacg
     3961 ggagccgggg gttcggagac cggcggcagg ccgatgcact gcaaggtgct gttgatctgg
     4021 acgtggaatt gctggccact caggtacgag tattgggcaa gcgagggatg gctcgggacg
     4081 gcctgggcgg cagccaccca gcgcaggcgc tggtcttcgc tcagcgccac gccccaactg
     4141 cgggaagcgg agccgaagat cgcgcgcatg cggttttggc gttccgtttt ggggtctcga
     4201 gggatcgtgc gggtgcgggc gcattgtccg aaccgggagg gataaaacac caccgaagca
     4261 agttttccgg tgcgagggga atccattatc ttcataaaca ataacctttc tcataacggc
     4321 cgggagcgcg gcggatctgc cagcccaacg tgagcttgca gcccgggccg ccctgcctcc
     4381 acagccacgg ggaaagggtt gccggaacgg gcatagacct caagccctta ttcatcgcgc
     4441 ataccctggg cgcgcaaagg cccgccggcc accgtcatcc ccctgcttgc ccgcgccgga
     4501 accgcgggcc tcgctttacc tgctggcgcg ggataaccgc ccccgggtgg cagcgcccac
     4561 gggaggtttc ccgattactc ccggtcgggt ttggaagggg gagcaagggc tcccgcagcc
     4621 gccgggtttt gcgggacgcg atccgcctgg ccaacggcca aagcctgcag aacccaggag
     4681 ccaatccagc gccggagcaa ccgccgccgc agcttgcccc ggccggcgaa cagggcccag
     4741 gaacgggcgc aggcggcgcg gtaatccgca aagcggccca ccttgtagag atcgcgcagg
     4801 tgctcccaca cctcggcctg ttcgaggagg cggaagttgg cgcccaaccg ctcccgcacc
     4861 cggggattcg cccgcaggct ctccagcatc agcaagccct gcgcggccgc cttgcgataa
     4921 ttcatgcgcg ccgagccgcg gacggcgtgg aagcggttca ccagggtgcc gcagcaccgg
     4981 aagtccaggc tcgccgccag gcggatcagc agttccttgt cctcgcgatg ggtgaggcgt
     5041 tcgtgataca tgccatgctg cagcatgttg ggacgcgaga gcagaaatgc gttgttatgc
     5101 acgaaggggt ggcggatata cgccagcaac acgtcgccct cgggagcggc ctggcccgcc
     5161 cggcgggctt cgtccgcatc ggtccccagg ctggtaaagc cataggccac gccgttgaac
     5221 gcgggatcgc gcagaaaaac gcccagccga tcccgcaccg agtgccgggg caactcgtcg
     5281 tccgagtcga gcatacaaac gtactccgcg gtggccgcgc gaatcccggc gttgcgagcc
     5341 gctgacttct ccccgttttg gggcagcacg atccggcgca cccgctcgcc aaactgcgcg
     5401 cgcacgtggc cgggggtgcc atcggtcgag ccatcgtcca cgacgacgca ctccgtagcg
     5461 gcatgagatt gcgccagcac agactcgatc gcacgcgcca ccaggggcca gcggttatag
     5521 gtgggaataa tgacggcgac ggttggggtg ggcatgacgg ttctccggcc tcagcggatg
     5581 agcgaaaaca gcgcgtccag gaacgcctgg cgggcggcag cggcgcgctc ctgaatgccg
     5641 tggcggacca ggtccagcag cgggcgggac gccagccgtt ccgccgtctc cgcggcccgc
     5701 cgctcacgcc agcggcgacg ggtcagttcg ccagcgtgga tgtagaattc gagggcctca
     5761 ttcatggcat aaaaaaccca acgcttgtcg cgccacgctc ccaccacccc gcaactgaca
     5821 tccatctccc ggcgggacca ggtttcgccg atgcaaatcg ccgggatccc cagccgccag
     5881 gcgttcacac agacatgata ggtgtcgctg atcacgaaat cataccggcg aatacggtcc
     5941 aggaggtcca ccagcgcggg tggcgcggtg gataccgggc gcgccagttc ggggaacgcc
     6001 caccgggagg gccattccgg aaaccgggag cttcgccagg gcagccactc cgcccgcact
     6061 ccgcgcgccc ggcaaaccgc gcgcgcaaac gccagcatca accgctgcgg cacgacattg
     6121 cgggagagga aaaggcccgc cacgccgcag tccgttcccg cgcgcgggtc ggcggcccac
     6181 tgccgcggga ggccggccag acttgcgtct tcgagcaaca gggcgcaatc cacgcccaaa
     6241 aacgactggc tccagtcatt ccgcagccgg ttgatcagcc gcgccgagaa gaggtcgcgc
     6301 atccagatcc cccgggtctg ccgcgcccag cggcgcaaga ggttttcgta ggccgggtcc
     6361 aggcggtcgg ccgcgcagtt aaagatgagg gttccgccaa acgccagtgt ctggttcagc
     6421 atcccgtctg gcgaatcggc caggaagaaa tgctgatcca cggccgcgat cccttccgcg
     6481 agcgttggcg tgattcccag ggcaaccaac cgccgggcga gttgctggcg gtagtgggcc
     6541 atgtgcagaa agtcgcccca gaagatcacg gcgcggccgg cggccaacgc gggaagccgg
     6601 ttgcggcagg agtgataagc aaacggcagc gcttcgcgcg cggcaaattc ctgctgctgg
     6661 cggggggagc gatgccgatg ctgttcgtcc gcggtgaaca actggcagaa gcgcacgtcc
     6721 acgtccggaa aatgccgctg ccacaggccg tagaacgcca aatccaccga gcccatgccg
     6781 gggttgggcg tgggcggcgt cgcgcaaaca acatgaacca atccagacat aaagctcgct
     6841 gccagtgcgg ttagcgcgcg gcggccaagc ggaggggcgg cgcctaggcc gccgggccgg
     6901 ccagttccca gcccgcgcga tactcccgcg ccaggcactg gttggcttcg gcgtcagcgc
     6961 cgatcttcgc ggcggcggcg tcaaaggcca gccgccgtcc ccccaaccgc aacgacaccg
     7021 cgcccagatt gaacgcgtcc gagatgggcc cggcaagcag aaagtcgccc acggtggcgg
     7081 ggcctccctt gcaggccgcc acccatgccg ccgcggcgtc acgccgggtg ggcggccgcg
     7141 tttgcgcctc cgccgtttcc cgcgcgctcg ccgccgcgcg aaactcgcgc atctttttct
     7201 ccgggatgat tttcggcgcc tcaccgcgga aacccgcgag aatcttcccg cggtcgccca
     7261 caaacatcat cccctcggac ggcagcgcct ggttgtcgtg ctccaactcc tccggcgtgg
     7321 gcgggcgcag cgagccgtcg taccaaaaca ggtcgagcgc gggccgggcg ccccgggcgg
     7381 caaacttgaa ccggatggtg caggccgcgg gaaaagagta gtcgttccgg actcggacgg
     7441 ccacctggtc ccgaaccgtg cagacgtggg tggggcatga ttccacccgg accggcgcct
     7501 ccaagccgaa gagctggaac accggccaca ggctataatg ccccatgtcg gccagcgccc
     7561 cgccgccaaa ctcgtaccat ccccggaaga cggcgtgcgt atagcaggga tggtacggcc
     7621 ggtcgcgcga cggccccagc cacagcggcc agtcgaaccc ggccggcacc ggcggctgat
     7681 ccgtcgggat cgcggagtat tggggccaca cggggcggtt agaccagtta tgaatctcgc
     7741 gcaaggagcc gatggcgccg ccatcaatcc agcgcttgat ttcctggatg tccgcgccgt
     7801 cgctggcggc gagaaaatgc gtggccacct ttgtcttccg ggcccactcg atcacccacc
     7861 gcgcctccag caggcggttg gccagcggtt tatgcatcat gacgtgcttg ccctgtttca
     7921 tcgccgcaat cgccaccgcc gcatgcaggt ggtcgggggt catcaccttg acggcattca
     7981 catcccgctc ctgcgccagc agctcgcgaa aatcggcgta ggctgtgcag ccccggaact
     8041 tctcctccga ccgctggcgg gcatagacgg tttccaccac ctcccgggcc acttcgcgcc
     8101 ccccggggat gcccggctgg ccgcgccgcc aatccggctg gcgcagcatc tcggcgatcg
     8161 ccgcgcgatg accgtcccgg gaccagtcca ggtagtcgtg cccgtccttg accggatcac
     8221 acacggcaac gatctgaacc tcgggcaagg cgaacaggct tggcaattcc cgcagcccct
     8281 gcgtgccgca accgatcagg gccagggtaa ttttttcgct gggcgccaca aaccccgcgc
     8341 ctcccagcac ccgccgcggc acaatcgtaa acgccgccgc cgctgtcccg cccgtcacaa
     8401 tgaacttccg ccgggaaacc ccgggagaac cggattgatg gctcgtcttg cctgctttca
     8461 cggcccaagt ctggcgcaaa actttctgcc gcgcaatcct tcccgaaccg tcggcacgcg
     8521 cttccgttcg gccgcccgga ctcccctgcc cccgccatat ccgcgcggga ttggcgcctt
     8581 aacaacggcg gacggccagg cgccagccac gtctcaccgg cagggagctt tcttcccggc
     8641 gaacggatta cgatcccggg agggcaaaag ggtatgggcc gcgctcagcc actctccgcc
     8701 gagcgccggg catgatgcta ttcggcgcgg ggcctacttc tttttgggcg cgggagccgg
     8761 ggcagttgga gccggagaag ccggcggcga aacagtcgct cccgtggagg tatccacctg
     8821 caaattgtat ttggtgagca ccggggcaaa gtccggatgc gtgcgcccga attcgatgat
     8881 cttcttgacg aaatcattca tggccggggc gctggttttc tggtagtcgg cgatcatttt
     8941 cgtggcttgc gggcttacgg ccgccaggtc tttgctcacg gttcgccatt gccgcagaaa
     9001 atagatattg agcgtcccgc taatcacaat gaccaggatc aagatggata ccaccatgtg
     9061 ctgcagcgcg tcatactgcg cctgcagggg gttggcctcg gcgggggagg agggtttatt
     9121 gtcgaaggag tccataagcg gctacttggt tgcgggtttg gttgcggccg gagtcccggt
     9181 tttagccggc ttcaggccga cggattccag gatgggatca atcgcggggt ttttcttgct
     9241 gtattccagc gcgtcattgg ccagggtggc catcaccgtc cggttgttgt taattatgtt
     9301 caactgcccc tgcagcgtcc gcagttggcg ggtattggaa atataaagcc agcacaacac
     9361 gaccgaggcg agcgcgctga ccagcagcac ccccagcagc accctgggca aaggattgtt
     9421 tttcatattt atggcgcgac tctaacggtt tttccggact tgtccatcct gtttttttac
     9481 ttcagaagcc gcttgatttt ggttgggcga aacggcatgt tatcgtcctt ggacaaccgg
     9541 cagcggccaa acgcgtgacg aacaggccgc gggccggagt ccggaaaata cctggtttac
     9601 attaaagact gtttagcgtg gcgaaggaag aaccgattga attaagcggc tcagtgacac
     9661 aggtgctgcc cggcaccatg ttccgcgtgg ccttgcccaa tggccacgaa gtgttggcgc
     9721 atatttcggg gaagatgcgc aagaacttca tccggatcag cgttggcgac aaggtcaacg
     9781 tcgaaatgtc cccctacgac ctgggcaaag cgcggattac cttccgccat agctgaccgc
     9841 cggcgccaac gacgccggcc ctgcccgcgc gcggcttttg ccaagtccgg ggcggatttg
     9901 gctcgacatc cgcgccggag ccaacatctt cccggtcatg cgaatttcat tttttggcgc
     9961 cacgcgcacc acgaccggtt cgctgtatct ccttgaatta aatgggcaac ggctcctgct
    10021 ggagtgcggc ctgttccagg ggcggcgcgg ggaatccatc gagcgcaacc gcaccttccc
    10081 tttcgacccg aaagccatta acgcggttgt cctcagccat gcgcatgttg atcattgcgg
    10141 caacctgccc aacctttgcc ggcagggatt cgagggaaat atttattgca cctttgccac
    10201 ccgcgacctg gccagcatca tgctggagga ttcggcgcaa atccaacggg atgacgcggc
    10261 gtttgtatcc aggaaacgcg ccaagcgggg cctgccgccc gtcgaaccgc tgtacaccgc
    10321 cctggatgcg gaccgggcgg ttcggcagtt cgtcagcgtg aactacaacc gcccgttccc
    10381 ggtttccgac ggcgtcacgg tcaccttcca cgatgccggc catattcttg gcgccgcgca
    10441 agtggccttc gacatccgtg aaaacgggcg ccaataccgt tatcttttca gcggcgatat
    10501 tgggcggggc aatgacgata ttctccggga tccggagcgg gtggaagatg tggactacct
    10561 tcaggtggaa agcacctacg gggcgcgcga gcatgcgccg aaaccccgcg ccaacgcgga
    10621 cgtcgagcgg atggtgctgg aaaccctcgg ccggcagggc aaggttatca ttccctcatt
    10681 ctccgtgggg cggacccagc aggtcgtcta tacgctgcat cagctaaccc tggccgggaa
    10741 attgccccgc gttcccatct tcgtggacag ccccctgagc gtgaatgcga ccgaagtcta
    10801 tcggctgcat cccgaatgct tcaacgaagc cacttacaag ttcctgcgcg aaaagcagaa
    10861 cccgttcggg atggagaacc tgacttatat ccgcgaggtg gcccattcga tgaagcttaa
    10921 tgatctgaag gaggcggcga tcattatcag cgcctcgggc atggccgaag ccgggcgtat
    10981 ccgccatcat ctggccaacc acatcggcaa cccggtgaac ttgattctct tcgtcggtta
    11041 ttgcgccgag cacaccctgg gcgcgcaaat cctggccggc cggaatcctg tgaatatctt
    11101 cggcgaaccg cacgaagtcc gggcccgcat tgcctcgatt gattcttttt ccggtcatgc
    11161 cgacaagcac gaactgcgcc gctacgtcga gaatatcacg ggcgaaatca agcagattag
    11221 cgtcattcac ggtgaagagg agcaggccct ggctttcggc gaaaccctgc ggcaaatgaa
    11281 accccaagcc caggtcctcg tgcccgagta tcggcaagtg caggagattt gactcccgct
    11341 ccccgccggc aaacgattcc gtcggccccg gccccgaggc gactccgccc caacgctttt
    11401 taatcaggcc ggccagggac cgcgcgcgcg ggggtgcatt tgccagatgt agtccgctcc
    11461 gggacggaac ccggaagcaa cgcggctaat ccttgtgagg gagcaggcag gttcttttcg
    11521 ggattcgtgg gttttatttg gcgtcttgcc gcccggttgg cgatctccta cctttcgaga
    11581 cgttttaatt atgtcagcca attttagcac cggcacgccg gactccgttc cggcgccgct
    11641 gcgacgggaa ttgtcctttg cgctcatcgg ctgcggccgg gtgtcggaaa accacctggc
    11701 ggcgctcacc gcggcggagg ttccggcccg cctggtggct gtggctgacg ttgatccatc
    11761 ccgcgcccag cagaaaggag ccaagtatgg ggttcccgcc tacacggact tttcggtcat
    11821 gctggcggag catccggaga ttgaggtcgt ggatatcgcc acgcccacgg gttatcattc
    11881 cgcgcatgta caggcggtgg cgcgtttcca gaagcatatc gtggtggaga aaccgatggc
    11941 cctgaccctc gaggactgcg atgcgatgct ggcggcctgc cgacaaaacg ggcggcggct
    12001 gtttgtagtg aaacagaatc gtttcaaccc cgcggtgcaa gccgcccggc gcgctttcac
    12061 ggccgggcgt tttggcaatt tggtgctggt caccgtgcgc gtgcggtggc gccgggacca
    12121 gtcgtactat gaagcggacg gctggcatgg ccggtgggcg ttggacgggg gggtgatgtc
    12181 gcagcaggcc agtcatcata ttgacctgct gcagtggttt ctcggcccga ttgagtccgt
    12241 ccagtgccag gcggcgaatc gcctcttgcg gttggaggcg gaagacaccg ccgtggctat
    12301 cttcaaggca gcgagcggcg cgctgggaat cctggaagcc acggtcgccg cccgcccccg
    12361 gaacctggaa ggctccctca gcattctggg cgagaaaggc acggtggtga ttggcggcgt
    12421 ggccgtaaac cagattctct gctgggaatt cacggagccg caaccggggg atgaggtcat
    12481 tcaccagcaa gcatcgcaag ccgtccccaa cgtgtatggc aacgggcatg ttccctatct
    12541 ccgtgatgtc gccgaggcca tcctcgataa ccgtccgggg ctcgtggaag ggaacgaagg
    12601 gcgcaagaac gtggagatcc tcacggcgct ctacgaatcc gcagccggag atggcgcgaa
    12661 gctaagcccc ggctgcccga tcgtgcgttc acggctgggc cgcggttaat ctaactcccg
    12721 gcttcacttt ccccctcccc ggcggcgcgc gctgtgaaca ttctacacct gttcagcgac
    12781 tggaaatgga ccggcccctc cgagcccgtg ctcaacatgt gccggggcct gcagcagttg
    12841 gggcatgccg tcaccctggc ctgcccgccc cgcgccgggc gcgaatcccg cagtatcgct
    12901 actttcgccc gggatcgcgg agtcaacctc agccaccaat tcgaactgaa tcaccatcag
    12961 gtgctgcgca ccagcctgcc cgacctccag cggctcacgc gcttcctgcg gcaccacccc
    13021 cttgatatcg ttcacactca cctttcgcac gatcacctcc tgggcggact ggccgcccgc
    13081 ctgagcgcgc gcccggtggt ggtgattcgc accgatcatt accgcgactc ctttgccccc
    13141 ggattcctga accgctggct gctcacccgg ctggcggatg gcgtggtgac cttctccgat
    13201 cgagcccaat tacgcctgcg gcagcagttt gggctcgctg ccgaacgggt ggcccggatc
    13261 agccccgccc tcggtcccga ggccttcact cccgtgcccg aaacggcccg ccgacaaatg
    13321 cgcgaggagt tcgggtttcg ggatgacgag gtcgtcctgg gcgtcgttgc ccgtttccag
    13381 aaataccgcc ggatggaact gttcctcgcc gcgctggccc aagccagccg gacagccccc
    13441 caactcaagg ccctgttggt cgggcgcggc ggcgacatgc agcagactgt tctggatccg
    13501 attcgccagt tcggcctgga gccgcgggtc aaagtggccg gctaccttac cgatcgctac
    13561 cgcgcagccc tggccagcat ggacggcatg gtctttctca tcgccggctc cgacggcacc
    13621 gcccgcgcca tgcgcgaggc catggccctg ggccgcccga tcatcgccaa caacctcggg
    13681 atgctgccgg aattgaacga atccggcaaa acggggctta tcttcaacga caccgccgac
    13741 gacctggccc gctgcatggt ccaactcgcc tctgacccgg cgcaacgccg ccgcctgggc
    13801 gaggctgcgg cgctcaaggc cgcaaaagag ttccggctgg acgcgcaagc cgtcgccctg
    13861 gccgactttt actcccgacg ccagcgttcc cgagtgcttt cggcagcgta attcaacctc
    13921 tacccttggg catatgcgcc tccctctgag gctcgcgaat tgagattggc gtttgcccca
    13981 aatccacacc atcttcccaa cccggttggc attgttatga gtgagaccca agcagatcgg
    14041 ctgccgccgc cgcccgcacc tgggcgacgc cgcgcaaaag ccgcgcaaat agccactctg
    14101 tttatcccgg tgacggtgct gcttttcttc gttatcagaa ccactggtta ccccttgctg
    14161 agccggcatg atgttcgcgt cggctcgtat gtcctggacg ccgtgcaaaa tggcaattgg
    14221 attatccaaa aggattacgg cggtgaaatc gcggccaagc cgcctctcct cacttggtgc
    14281 gcggcggtga cgacgctgct ggccggccga atcagccggg tcgccattca tctgcccgcc
    14341 gcggttgccc tgtgcgccac ggccctggct ctgctggccg gcggacgcag ccgctttggc
    14401 gaacgcgccg gctttctggc cgccatgatt tatgtggttt ctccggccgg ctactcgcaa
    14461 ctggccactg cgcgctatga cggcttgctg gcgttcatgg tcgttttatc agccttcgcg
    14521 gctttccgcg cctggcattg cggttcgggt tggatttggt tctggcttgc cgcggcagcc
    14581 ggcaccctgg tcaaggggcc gctggcggtt ctgttgggtg gagcgggcct gctggcttat
    14641 ttctgggaat ggcgcaccgg ttcgcgcctt cccttgcgag gcaatcattt ccccggcttg
    14701 cttttgtttt tgctgatctg cggcggctgg tttctgctgg catacgcgca gttgggccag
    14761 cccttgatca acaagatgat tgggcgggaa ctgatcggcg cggcggtggg ggctgaggac
    14821 gggccatcgc cctgggggcc cctgaaaccg ccccgggatt tcctgataac ctttgcgccg
    14881 tggagcttcg cctgcttgta ttcgttttgg cgcatctgga aacggccgga cgcggaccca
    14941 acacgtcgcc gctttgagcg tttccttgcc agcagtctgc tggcgggaat aattgtcttt
    15001 agcgcggggg gacatcgcca gagccggttg attgtgcccc tgcttcccct cgccgcgctc
    15061 cttgccggcc gcgaacttgc cacactgctt gctctctgga gcgatcgcaa actgtttcgc
    15121 ctggcagcgg tattggccgt cggaatggga atcggcatct tcattcatgg gcattttctc
    15181 atgcgccgca gcccccgggt gacggaaaca tctgccgccc ggcagacggc gcgcttgatc
    15241 cgtgaacagt tggggccgga ggcgcccttg acctatgtgg acagcccgtt tgccgtgcaa
    15301 ttctacttga acgatgttcg cccggcggcc tctttcgagc gagcggcggc attgctgcgg
    15361 ggcggcgcgg gcgcgctggt ggtggtccgc gatgtggatc gcttgaggaa acaaatgggg
    15421 ccggatgcgg atcgccttca tgaaatcctg cgcctgcccc caacgggaga gccttggata
    15481 caggtccttg gcaaccaacc ccagcccctc acgaaccagc cggtggccct gcttctgggt
    15541 ccgcttcggg tggaactcga ctctcttcgc ctgcagcgtc tgcgaatcaa ttatcggcac
    15601 ggcatagatc tgttcgtcca cggcaacccg gccccgggaa agcttcgggt cgagaaccaa
    15661 agcgcttctc ctcaatgggt gcgcctccgt ctgattgagg ccaagtcggg cagggtgatc
    15721 acggaaacga aacgctccct tgcgccggga acggtatggg atttccccca ggaccgccct
    15781 ctggccctgg gaaaaggcgc ggggccattc accctcgcgc gcgggccgga ctcccggcct
    15841 ccggctccag caatctcacc agccgggcca tgacctgcgt cttgtcagtt ttcccacccc
    15901 gcaaatagcc cagcaaacgg cggattatgc ccgccagccc cccccgttga gttgcttccc
    15961 gggccgcagc ccgcaccagc ggcggattcg cgtagccccg cataagcgct cgcaggaact
    16021 cctcgccacc accgccatcg gcccgcagca acgaatcggc aatccccgtc aactcccgcc
    16081 cgatagccct aaccaccggg tagccggacc gataaccgtg ctccaagtcg aacgcgatca
    16141 accgtttgtc ctgcgcgaag aagtgtttga tctgcccatg ctcatgaacc agcctcggat
    16201 cgttaagttc cagtgcccgg cgatgccgcc ggcttaacgc ctggcccagc aactccaccc
    16261 acgcgagttt ttcacctggc ggccggttcg gatcggccag cactttcgcc agcactggag
    16321 ccgcgcagta ttccagccac agcgccggct tctccatccc ggaaggcagc ggtcgcgcca
    16381 ccaggcggac cacatcaaag ccttcgcgcg cccatagatc cagaataagc ctctcggtgg
    16441 cgcaccgggt tgcagccgtt gctccccgtt tgccttcgaa cacccgttcg gaaacgtctt
    16501 tccagaactc acgccagcgg gatcgccgaa aacaatagac cttgagcacc agcggcggct
    16561 gctcggaggg gaaaaaatac aacaggtttc cggtttggcc ccgcccgccg gcctgcagca
    16621 cttccgctcc cgtcggcggt tctggtcgtg atcgtttcat ggcgttggag agcccgggat
    16681 actgcccctg gaatctttga gctcaagctt ttgcccgctc cagctcttcc cggagcaagc
    16741 gtgccacgct gtatttgctc gtcgggctcc tggctttggc cttaaaggct cgttcgcagg
    16801 cacgtccaaa acgatgcagt agatttgggt gccgccaaaa gtactcatac gcccgcccga
    16861 gccgttcttt ttgcggataa tgccgcaccg tttccgggat caaccgttcc cggattcccg
    16921 gcggcacgct cttccacagg aaccacaaat attgaatgat ctcgtgcgac acgtgctccg
    16981 ccactcgccg gcggcttcga aacgtcatct caaagtcgaa ccacaggaac tctccgcccg
    17041 gaagcagcat cacgtgtttg ccgtcgccat tctcgtgcgc caggcgcggt tcacgctcgc
    17101 gaaccgcaat atcgtgccgc cgactccact ccagcaacca ggcccggtaa accgccaatc
    17161 gttcttccac gctgcgggat tcgtccttca tccaatcgcc cagcttcggc gcctccacgt
    17221 attcgaacag catatatccg ccctcggggc atcccggcgc ctcaacccgg acctcaggat
    17281 acgtgccaaa cacgcggaag ccgtgtttcc gccaaatggc catgcactcc gcctcgaccc
    17341 gccgccgggt cctgggcaaa tacgacgtct gcccggccag caccacgttc tcaaagctct
    17401 tggccaggca ggaaaaccaa ctacggctgc cgtaataaac cttcaacacg gcccaaccct
    17461 cgcgaaacgg tatcttgtac aaataattgc cattctgcag cagcagatac ggcttcaaat
    17521 ccggcaaccg gatcacttca ttgctttggc tcatgcaggc ttttcgactc cgtcgcccgc
    17581 gcctggactt ccaagggcga cagcgccggg cgttcggggc atgaaactac gccaggccga
    17641 aaatgtcaat tcccccgtca tccaaaccgc ccaggaagca gctcgtttcc ggtagtgcaa
    17701 gagcagatgg ctattcgaag gtctggcagg gacgccttcc tcaccccacc cgccgggcct
    17761 catcgggaaa gcaaccgcgc gtagagttgc aggtaagcct ggaccattgc ttccacggaa
    17821 cgctcgcgtt gaacccaatc ccgcgcagcg gttctcagcc gttcggccaa ctcggcctcc
    17881 tccaacaggc gtagcatcgc ctccgctaat cccgcatgat cgcccaccgg cacaagcagg
    17941 ccggtttcgc catcccgcac cagttcgggc agccctcccc cagcagtggc cgccacgggc
    18001 acgcccgcgg caaaagcgtc cagcacgatg ctgcccaatc cctccatgca cgaacttatt
    18061 acaaacacgt caaatgcggc cagccactgt tccgcctgcg gaataaaccc cggcattaac
    18121 accgcctctt ccagctccaa ctcccgtcgc tgcgccagca gtgctcctct cagttcgccg
    18181 tcgcccagaa taacaaatcg cacatcgcgc cgccgcgcga ttagcccctt ggcggcgcgc
    18241 agcagggtcg cctgatcctt atgccccacc agggccgcaa tgttgcccac aagtttggtg
    18301 cccgggccaa tccccaagcg ggtacgcacc gctgcccgat gcacgggggt cagcagccca
    18361 ggggccggca tcggcacggc gctgcgaatc acgcacattc gctcgggggc aactccccag
    18421 gccgccagtt ggttggcgat gaactgcgaa atgcacacca cctgcgccgc gcgctgaaat
    18481 ttatagcggt tgaaccagcc tcgcctcggc agaaagtcca cccggcgggt cgccaggaat
    18541 ggcttccgat atccgggcgc agtcaaggcc gccaggctgt gcccgcgccc cgtgtggcaa
    18601 tggatcaagt cgaactccct agcgagtcgc ggcaaggcca gccccgcccc cagattattc
    18661 cccggaattg cgcgaaccgg caccccggct tccagtgcct tccgcgccag cgggccatcg
    18721 gaacggcatg caatcacgga cccaatccca cacgctctca acccagccgc cagcagcagc
    18781 gtctggcgtt cgccgcctcg ccaggacttt tcgttgttca gatggaggat attcatggcc
    18841 accaggcccc ccacaagccc ggagggctct tctttcttcc acaatcagcc atccggcaca
    18901 atctctttat taaccccctc gcggcctgga ttcggatatc cttgccgaaa ggcaaaaata
    18961 ccttgtcgcc gctcgcggcc ccatttacca atggcgcatg cagagtcgtc tgccgctttc
    19021 agtcgccgtc atcaccttga atgaagaggt gaacctgccg cgctgtctgg gcagcatccg
    19081 cgacctcgcg gcagaactgg tgatcgttga ttccggctct accgatcgaa cccaggaaat
    19141 cagccgccag atgggcgcgc ttttcatcgc ccagccctgg ctgggtttta ctcggcaaaa
    19201 gaacttcgcg ctcgaacgtt gctcccagcc atgggcgcta tgcctggacg ctgatgaagc
    19261 tgtgtccccc gaactggccg aggcaatacg gcacgccttt gcttccccgc cctccgttaa
    19321 cggctttgag ttgaaccgcc gcaccagcta cctcgggaaa tggatctggc atgcctggta
    19381 cccggaatgg tgcctccgcc tggttcgcca ggaagcggcg cgctgggccg gagctgatcc
    19441 tcacccctcc ctttcagtca cgggcgccac tgccaaatta tccggcgatt tgcttcatta
    19501 ctcctacgga gatttgcggg agcatttgga gcgcaccctg cgctatgccc gaacctcggc
    19561 ggacgctatg gccttggcgg gtcggcactg ctgctggtat caccttgtca tttcgccttg
    19621 gctcgcgctg ggcaagcgtc tcattctgaa gcaaggtttc cgggatggct ggcgcggatg
    19681 gataatcgct tattcttcct ttctgtcggt tttcgccaaa tacgcgttcc tggcggagca
    19741 gcagcggctg gcgcaacgcc gcccgcaacc gcccaccgat cttccgacag gaacatcgtc
    19801 cgcttccaag ccctccggtc cggcttgatg ccggggcgag gccgtggcgc ggccgcctca
    19861 ggtctccact atttccgccg aaccgccact cggcaggatg ccttgtcgcg ccgccatgct
    19921 cgcgaaaact ccaccccgca gcaataactc atcaaagccg ccagattcta caatccgccc
    19981 ctcagccagc acaatcaccc ggtccatcgc agccagcgtt gagagacggt gcgccactgc
    20041 gatcaccgtc cgatgctccg ccagccggtc aatcgcagct tgcacctccg cttccgcttt
    20101 cgagtccagc gacgccgttg cttcatccag caccagaatc ggcgcgttgc ggataaaagc
    20161 ccgcgcaatg cacagtcgct ggcgttgccc gccggacagc gttactcccc gctcgccaat
    20221 ccgcgtctca tagccctgcg gcaacgatag gataaactca tgcacaaaag cggcccgggc
    20281 ggcggcctct atctgctccc gcgtcgcgcc ctcccgaccc agggcgatat tctccgccac
    20341 cgtcatatcg aaaagcacga tgtcctggct caccaaggcc atgagttgcc gcaagtcggc
    20401 attagctatc tgccgcaaat ccactccatc tatccggatc accccctgga ccggatcgaa
    20461 aaaccggaac agcaagttca ccagggtact cttgccggaa ccgctctcgc cagccacccc
    20521 cagccgggtg ccgcgcggaa tctccaggct cagttcgcgc aacaccggtt cccgaccata
    20581 gctgaagctt acctggtcaa aaacaacgct gcttttaaac tccagcagcc gccggggcgc
    20641 cgccgcctcc ttgaccgtcg gccgttccgt catgatctgc agcaaccgct gcaccccaaa
    20701 ggcggtctgt tcaaacatca cgtgcaattg cgccaccttt ttgaccggag tataaaagaa
    20761 aatcaggccc gtcagaaaac ccaccatgtc cgacactggc cgattctggt aggtaatgta
    20821 aactaccagc aggccaaacc ccatcactga cacggtttcg attatgggat ttactagttc
    20881 cttggcgcgc acgcctttga ggttttgccg taccaactgt cgtgaaaacc gccggaaccg
    20941 ctccacttgc atcgcctcca gcccgaacgc tttcaccacg cggattcccg acagaatctc
    21001 gtatagcaag ctcgcctgag tcacccccac tttcgtgctc gcgcgcgcgg cgaggcgcgc
    21061 ccgccgaccc atcaccacca cgggaatcac gcacgccgga aagaaaatca tcgccgccag
    21121 cgtcagccgc cagtcaatca cgcacaacgc caccagcacg cctatggcgg tcatcggctg
    21181 ctggaccagg tccgcgcaac ccacccgcag gcagcgttgc agcgttgcgg tgtcgccatt
    21241 tacgtgcgtg accatgtccc ccattgtggc gcggttaaaa taatccaggg acagggtgct
    21301 gagcctgatt aatacgtcgg ttcgcaaatc attcaccacc tgctcgccca cccaggccat
    21361 gcaatacgcg ctcaggtagg ccatgattcc gcgcaccgct accagggccg ggaacaagag
    21421 cagccccccc agcacctgcc gccaatccac cggccgcccc gcatacggca gccacggatc
    21481 cacccattcc cgggttcgcc gatctaattc cgcctttatg cggtccagct tctgctcccg
    21541 gccaggcttc tcttttttca agtgcgcccg agcctccgtt tccggcgcca tccgcccgat
    21601 caacgtcttg gtgccccaca caaaagtcgc gctcgtcgcc ccaaatagaa tcccaaacaa
    21661 cacgccggca gccagccgta tccaataacg gcgcagatat ggcaggccaa agcgcagcac
    21721 cttgaggatg tgattcatcg cgtcgcggta tgctcccgaa ttcccgtccg gagtcaatcc
    21781 gtttgatgca aggataccga ctgcttgaca gggatgaggt ttgcaattaa agacatggag
    21841 caatgcgcgc gcgcgttcgg cggggggcgg tttcgttgcc cctggcagcc ccgccggaaa
    21901 gccgctccaa ggtcgcgcaa ccgcgatggt ttgggagaga tggccgagtg gctgaaggcg
    21961 gcggtttgct aaaccgttat acggtttaaa gccgtatcgg gggttcgaat ccccctctct
    22021 ccgccattcc cgaatggaaa caccggcgcc cgcccaccca caccctcaac caaataagtc
    22081 catttgtggc ggcggggcaa gctgcttgag ccggtcgcgg tcctgttgtc ggcgcgtggc
    22141 ttggcgcacg ttgccttcgg gggtgagttc ggattcctcc aggttgacca ggatttgctt
    22201 cgcccgctcc aacacactcc gggggacccc cgccagcctc gccacctgaa tgccataact
    22261 cttgtcagtg ccgccagcca ctatcttgcg cagaaaaata atctgctcgt gccactcgcg
    22321 cacggcgacg ttgaaattct tcagccgggg cagccgcccg gccaattccg tcaattcatg
    22381 ataatgagtc gcgaagagcg tctgggcacc gaccgtgttg tgcaggtgtt caacgatgct
    22441 ccacgccagg ctcaacccgt caaaggtgct cgtgccgcga ccgatttcat ccaggataat
    22501 caggctgcgc ggggtggcat tattaaggat gtttgccgtc tcacacattt ccaccatgaa
    22561 cgtggattgc cctcgcgcca gatcgtcgct ggccccgatg cgggtgaaaa tgcggtccac
    22621 cagccccaca cgcgcctccg ccgccggaat gaacgacccg gtctgcgcta gcagcacgag
    22681 cagcgctacc tggcggatgt aggtgctctt gcccgccatg ttcggcccgg taataagggc
    22741 aatctgggtt tcgctcccca ggaaagtgtc gttcggcaca aaccgctcct cggtcaggct
    22801 ctgctccagc accggatggc gcccgtcgcg aatatacagg ccgctcgcct cgcccaactg
    22861 cgggcggcaa tagttgtgaa gccgcgcggt ttcggcaaaa gcgcccagca cgtccagttg
    22921 ggcgagtgcc gccgcagtct gctgaagttc gcgcaaccgt tcgagcacgg cctcgcgcac
    22981 gcgctggaac aagtcgtatt ccagcttcac cccgcgctct tcagcgccca aaatccgccc
    23041 ctccatctcc ttgaggtctg gcgtgataaa ccgctcacca ttggcgatgg tttgcttgcg
    23101 gatatagtcc ggcggcacct tgtccaggtt ggctttggtg acctcaaggt agtagccgaa
    23161 gaccgagttg aaccgcactt tgagggacgg gatacccgtg cgcacaatct cgtcctgctg
    23221 caacttcgcg atccaatcct tgccctcgcg catggccgcg cgcagttcat ccagcgcggg
    23281 gtcgaagcca tcgcgaatca ggccgccttc ctttatagcc agcggcggct catccacaat
    23341 ggctcgtgct atcaactccg ccagatccgg caactccgtc acctgaccgc caaggtcctc
    23401 cagcaaaaga ggatggcctc ccacatgaac ctcgccttcc gataatgttt gaacgggcgg
    23461 aggcgcttgc tctcccggcg ccgccggcgc aaggaggcac aactctcttt cgctccgccg
    23521 gccttcctcc gccgcgtttc ccgttgttaa ctcgcgcacc aaccgcttga ggtctggcaa
    23581 tcgctccagc gcggcacgga gcgtggccag gtcgcgggcg ttgccggttc ctgcgctgag
    23641 ccgccccagc gtgcgttcca ggtcccgcac ttgcgctagc tgcgctcgca gctcttccaa
    23701 ggccgcgcta tgattcacca gcgtctgcac cgcttcttgc cgccgctgaa tgggcccgac
    23761 cttggccagc ggctgagaaa gccaatcgcg cagccggcgc gcgcccatgg gcgtgacggt
    23821 gcgattgagc gcgccgtaga gcgatgcatt tcgcggcgta tccgggtgca gcggttccaa
    23881 aacctccagg tgccgcagcg tcgaaaaatc gagcgccagg tagtcactgc gttgataaaa
    23941 ggaaatgcgc gtcaggctgg ccgcatcgcg ccgcagatgc tgcgtcaggt aatgcagcac
    24001 ggcgcccgcc gcgccaatcg ccgccgtccg atccttcagg ccgaacccgt ccagcgaggc
    24061 aaccttgaaa tgctcgcgca cggtgaacaa agccgtctcc ggcgcgaaga cccagccatc
    24121 gtatccgttc agtatccagc caaatcccgt cggggcgaca tccgcgtatc ccaccccttc
    24181 cgcgccggga ctgccggctt tcgcaggagg agcaggcgtc ctcgccaacc ctgattttgc
    24241 gggaccggca ggaaaggcag ggctggcggg gtcgcctggc ttccccgggc ggtttgcgcc
    24301 ctgcagcaaa tccctcaccg ccccggcttc ggccggataa atcaattccg ccgggcgcag
    24361 gcgctggagt tccgtcaaca cttcggcctc gcccgccact tcggtcgcca tgaaatcgcc
    24421 cgtggtcagg tccgcaaacg ccaggccata gtttcggccg acggcataaa ccgcgcccag
    24481 gaaattattc cgctcagcct taagcacgcg ttcgtcgaaa tgcgtgcccg ggctgaggat
    24541 ttgcgtcacc tcgcgcttca ccaattgacc gggccgggct tcttccactt ggtcgcaaat
    24601 cgccaccttg cggccggccc tcagcaggcg cccgatgtac gcgctggcgg catgaaaagg
    24661 aatgccgcac atcggcacca cgccgcgttt ggtcagcgcc acgttcagca gttgtgcgcc
    24721 agtttgggca tcctcgaaaa acatctcgta gaagtcccca agccggaaca tcagcagcgc
    24781 gtctttgggc aattcgccct tgatgcggcg gtactgcgcc atcatgggcg tgagttgggc
    24841 gttgctggcc atcgccctta accctagccg gttcccggcg acaggtcgaa acgaaattta
    24901 ggccgcaaag acctgcggca gcgagctaac gttaaaaccc tcccgctatc aattccccgg
    24961 cactatcaat gtccgcaatg gcttgaccga tgcaaagtgg tttcctcgtc ccgccggcca
    25021 gcctggcctg ccgggcgaac cggaaggacc aacccttcgt cagcgacaat gtttttggcc
    25081 ttcatccccc tgccactgcg ttcccgcagc acgccccctc tctggcattg gctatttcgc
    25141 ccgcaacccc gccataacct gcgccattgt cagtgccgtc tgggcggctt cgcggccgcg
    25201 gttgcgctgt ggatccagac agcgggcgcg cgcctgggcc tcgttttcca gcaacaacac
    25261 ttcatgaatt accggcactt cataggccac ctgaacctcc atcagtgcgt ggctgacggc
    25321 ctcgccgatg tgagcggcat gaacggtttc gccgcgcagg atcaccccga gacagatgat
    25381 cgcggcaggg cggatgccgg aggaggtgag agactgggcg tgaggggagc aatttcgggc
    25441 gagacgcgcg gcgaccaggg gaatctcata cgcgccgggc acccgcacaa tcctgatctg
    25501 tttagctccg gcacgcttca attccgaaac ggcggcccgc atcatagaat caacataccg
    25561 gccgttgtaa cgcgaggcaa cgatggcaaa gcatccgccg ctggcctgca atttcttctt
    25621 cttgatggtt ttcagcatat cacttcacga tggcgagacg gcccggacct aaaggaggtg
    25681 gccgagtttc tcgcgcttgg tcttcaaata gcgggcatta tgcgggttcg gctttacttg
    25741 gatcggcacc cgctcgatga ctttcaatcc gtagccctcc agcccgacaa tcttgcgcgg
    25801 gttattcgtc agcagacgta tgcgcttcag ccccaggtcc gccaggatct gtgcccccag
    25861 gccatattcg cgcaaatcca ttgcgtaacc gagtttctgg ttggcctcga ccgtatcgta
    25921 tcctcgctcc tgaagcttat aggctttgat tttcggggcc aggccaatgc cccggccttc
    25981 ctggcgcata taaaccacca cgccgcagcc tgcctccgcc acttgccgca tggcctggcg
    26041 caattgcggc ccgcagtcac agcggcagga actgaacaca tcccccgtga ggcattcgct
    26101 gtgaacccgc accagcacat cccgcttccc cgccacgttc ccgcacacga gcgccaaatg
    26161 attcaaccct tccagcttcg agcggtagag atacaaatcg aacttcccgt attcggtcgg
    26221 cagcttcacc acttcgacgc gctcgaccag tttctcgcgg gcccggcggt attggatcag
    26281 atcggctatc gcgcagacct tcagccgatg cttgcgggca aacttcatca gttccggcag
    26341 ccgcgccatc gtgccgtcgt cgttcatgat ctcgcaaatc accccgatgg gccggcaacc
    26401 ggcgagctgg accaggtcca cagccgcctc ggtatgcccg gcccgctgga gcacgccacc
    26461 gggccgcgcc cgcaacggaa acacatgccc cggttgcacc aggtcttccg gcacagcggt
    26521 cgggtccgcc agaacccgga tcgtccgcgc ccggtcggcg gcgctaatgc cggaggtgat
    26581 tccgcgcgcg gcgtccacgc tgacttgaaa gtccgtcttg aagttctcgc gattctggcg
    26641 caccatccgc tcgatgccca gttgctgcag gcgctccgag gtcgtcggca cgcaaatcaa
    26701 cccccggccg tgccgggcca tgaagttcac caccgccggc gtcacaaatt gcgccgccgc
    26761 gatcacgtcg ccttcgttct cgcggtcggc atcatcggtg acgatgacca tcttgccgcg
    26821 ccgcaaatcg gccatgacgg cttcgatgga attaaattgc tgcgccatat ctcagcccta
    26881 agctaccgaa gcacgccata attgccagtt ccaaaagcgc cacggctcag cccctgcccg
    26941 gtccgccgcg cgaccgggcc ggccacctcc cggcgccact ccagcgaaga ggcgtttgcc
    27001 cgacagcgct taaaccggcg gaacgtccgc gcccgggcac tcgctcattg cgctggacac
    27061 ttgatggcgg tgaggctgcc gtcgaagctg gtcaggtagg cgtgggtgcc gtcggaaacc
    27121 accgaaccca tgacgtaaag ctggggcgaa gcctggtatt gccacaagat tttgccgttg
    27181 tccgcagcga tggcggaaac aactcccttg ccgctgcaaa catagacgac gccgtttttc
    27241 tccgttggcg cggtggggat ggcgccgagg agcgccgcca cccgccagat ttccttgcct
    27301 gagctgtcca ccttggcgag ttcgccattg gttttgcgca gataaacgtg ctggccatcc
    27361 tcagagatgc cggtggccga aaccccggtc attgagccgg cgggctcgcc agtctcagcg
    27421 tcgaggatgc tcagcatcat attgcgatcg gcaatcatca ccttgccttc ggcgacgacc
    27481 ggcatggcgt cggcggggct gtaatagcgt ttggcgccct tgacgacgcg tgagccttcg
    27541 cccagttgct gccaaaccaa tttgccatcc cgggcgttga cgcagcgaac atactggtcc
    27601 caggcgccaa agaagacctt gccgttccaa acaaagggct tggactcgat ggcgtaggtg
    27661 gcgtccttgt tggtccaggc gagcttgccc gtgcccgcat ccaaggcgta gagccagccg
    27721 ttattgcagc cgaagatgat tttgccgtcc gccagcacgg gggaggaata gaccgcatcg
    27781 ccggcggtga acgaccagag cttctcgccg ctggtggtgt aagccgagac gattcccagc
    27841 ccgttggcgg tatagaccgt tcccttggcg attagcggtt ccgccaggat ttccgcgccg
    27901 gtgcgaactt cccacagcgc cgcgccggtg cgggcattga acgcgcgcag ggcgccgtca
    27961 ttcgccccca catagaccac ctcgtcaacg atggccggcg tgactttgct ggaagctccc
    28021 aggtaggccc gccaggctgt gggctgttgt tccggctcga aaaagaactg cgtgctgcgg
    28081 ctgaaccgct cgctgccctg catgaactcc acccgcagat agtgcgcgcc gggcaatagc
    28141 cggcttacct ccaccgtgcc gtccgcagtc cagttcggcg ccgtccccga aagtttgagg
    28201 tctcccttga tctcatcatc cacgctgtaa gtagccttct caagggcaac ggctccggac
    28261 aggcgcgcgg aaacgctcac ggagctgccg ccggcctgcc ggaaagcagg ggaaatgatt
    28321 tcgattttag ggtaggatgt ttgctccgga atgggttttt ccagcagctt caatccgggg
    28381 gccggggcat ccgctttcca ataggccacg cgcagcatgc cctctttgac ggacaccacg
    28441 gcgaagcccg cattggggcc gaaagtggag ccgccggtgg tctggtctat gccttccacc
    28501 aggcgatgcg catatccgtg cgagtggccg gccatcatca gcacggtgtt atagccgtgc
    28561 aagacgtcca gcagccggtc gtagtcgtag cggctggcaa actccgaccc cggcagcgga
    28621 tggtggaaga aaacaaacac cggtcgctgg gggccgactt tcgccaggtc ctgcttgagc
    28681 cagagcagtt gttcttcacc gacggacggg cggggatcct gcagggtggg cgtcatcagg
    28741 ccaacgaagt ggcaggtatg actgtcgaac gaatagcaag cggccaatcc ggcgtcacgc
    28801 agcgacttca aattggaccg ccaggtatta tcgtggttcc ccagttgtgg aaacaccggg
    28861 atggtgattc ccttccaata agcctggtat tcctcccacc agccgctgcc accgccgaac
    28921 tcattcaggt cgccggtcgc caacacaaag ctgggctttg gaatctcgag gccgtacggg
    28981 gccaggtcaa tcggccccaa accgacgatt tgggcgaccg tcgtcttgct ttgagccatc
    29041 ggcgcatgca aatcgctcgt atggatgaac gtaaagtctc cgaccgcccc gcggctggag
    29101 gaaaggacaa gtagtgccag cagcgtcccc aacgccgctc cgctgttctg ccgatgtttc
    29161 gcgaacctgt tcatgctgtc agcgtaaaaa gcgcagcaaa aggaagcaag cccttgaaac
    29221 aaggaaatgg cgccgggcca aagcgggcgc ggacatccct ctccacccgg cggaactgcg
    29281 ctccgggaat aacccgcata cccgcgctcc ttccggggct tcccgcggcg cgcggggccg
    29341 ccggggcaac cctgcggact ctgccaccca gcacgcggag cgcctaatcg gtctttttga
    29401 cgaacagcgc gagcgaggcg gtgtggccgt gcaggaagct cttctgcccc accgggccga
    29461 tctcgccatt gcagaagaag cccgccaggc ccagcggccc gaatcgttgc tggaccattt
    29521 gggcgtcgtg attgggctgg ccaaacaggc tttggccgcg cccgttgcaa ctgcacaggc
    29581 agccgccata aatcggggta tcgcccagtt gctgcttggc gcgcgcgagc aactcgcgca
    29641 tgtcctcatc ggcggtagcg gccgaacgcc gctggaactg gatggtttgg cccaggcgcg
    29701 gcagggcgcc aacggcgatg ctgcccgact gcgggtcggc gccgagcaag ttgcggatca
    29761 ggaaatcgcc gcgctggaaa tcctccaggt attcgttaac caccaggccg ataaagagat
    29821 tgccgcgggc ggtttcctgt tggtcggagg ggagttggtt gaaggtctcg gccagcactt
    29881 cataggcggg ccggttgccg attcggtgga tgatgttctg ctccaccttg gtaagggtcc
    29941 aggtctggcc aatgggggtg caggcctgcg acgtcacgcc gaccacccgc accgcgccgc
    30001 cgacggaaat cgccacgccg ccctccgggt acacctcgcc gttcagatac acctgggtgg
    30061 cctgcgcgtc ggactcgccg ctggccaggc cgcccaacac cggcaacggc gcgtaagcct
    30121 cgttccaggt gcggagccag gtttcgctgt ccaggtggaa cgggttaatg aaggtcagcc
    30181 agccgttgct ccggtcgggc tcgaccccgg tttccaggtg ccaatagccg gggccgttgg
    30241 cttcctcgac ctgctcctgc gaaaaatgaa ccgcccgcaa gtcggccccc ggcagcgcat
    30301 aaagccccag ggtcaagccc gcgtctttct ccacttcatg gtcgccgacg atcaggctct
    30361 ggctggaaca gcccgccagc agcgggatgt gagcatgcac gcgcagaatc tccagcacct
    30421 gctgcgcgcg atcgaaaaag cgcggagaca tgaaaaccag ccccagcgac acccgcggcg
    30481 cggccaactg gcgccgcaga ttctccgccc atttctggag gcccggttcg ctaaactcat
    30541 gcggccaatg cgccgcggtt gaatactcac tgcccacagt gccaccttag cggggaagcc
    30601 gctgaaagca accccggttt tcgacgcccg gccccagccc cgggcgttcg ggatcgaaaa
    30661 atgcggaacc gcggggagtc cggcgtccac ccccggcgtg gcgagggcgc ccgcaggccc
    30721 tgccgccacg gctgcctccg ccagcgcctt tccccgcacg gctccaagcg gccaggagtg
    30781 gaggcggccc tggcgccgga gggtgctttc ccgcatgcct ccatgccgtt gcccccgcga
    30841 aagccgccgc cgccgcatcg ccatcaggaa gcgcggcggc caagctcccg ctccgtcttc
    30901 aatcgcaact gcccgcaggc ggcgtcaata tccccgcctt tttcgcggcg cagggtggcc
    30961 ttgaccttct gtttcgccag cgcctgaagg aaagcctcct gcacatcctc ggccgggcgc
    31021 tcccaagcca ggccctcgac acgattatag ggaatcaggt tcaccttggc cttgaaccgg
    31081 cgcgccagag ccgccagcgg caggacctgg tccaggccat cattcacgcc ggcaatcaga
    31141 atatactcga acgtgatcat ccgcccccgc ttccgctggt actcctcgca ggcggcggtg
    31201 agctcgcgaa gcggatactt gcggttgacc ggcatgatcc ggctgcgcgt ctcatccgtc
    31261 gcgccgtgca gggaaatcgc cagccggaac tgctgcggct cctccgccag cttgcggatt
    31321 tgcggcgcca acccgctggt cgaaatggtt attttccgtg cgccaatgcc cccgccccac
    31381 ggcgcattca gaatccgcag ggctttgagc aggttatcga aattggccag cggctcgccc
    31441 atacccatga ttaccaggtt gcttaccaga cgggccgggg aggcgaacgg cgcgccgtgt
    31501 ggcacgaagg atgcgccggg cctggcgccg ggcgcgtctt gcgccctccc gttctcaccg
    31561 ccagcctccg gtcccggatt cccgaagccg gggttttcgc aggcggcccc ggaggcgctc
    31621 tccccggcgt tccaccgctc gacggccagc acctgctcga cgatttcctc gacgcgcaga
    31681 tttcgtttcc agcccgccag cccgctggcg cagaacttgc acccgtaggc acagccgacc
    31741 tgggtggaaa cgcacagcgt atgccggtcg ctgggttcgc cataaagcgc cgggttggct
    31801 gccatcagca cgctctcgat gagcgcccca tcgctcagcc gccacaaaaa cttctgcgtc
    31861 gcgtcccgcg ctccctgctt ccgcaccaac tccagcaccg ccagcgagta atgccgccgc
    31921 aactcctccc gcagcgcccg gggcaggttc gtcatggcct cccagcttgt ggcgcggtgc
    31981 gcataaagcc attccagcag ttgctgaacc cggtaagccg gctggcccca ggcccgaaac
    32041 tggttttcca gttcctccgc cgtctgcgat ttgatgtccg tcagcacccg gacaaagtag
    32101 cgcagagccg ccccggcaac gagaccctat tttaccctcc gatctcccgc cccgggaagg
    32161 ccggcgcccg accggtcgaa gcgggcgggc tacgtcttgg gcaggtaata cttctcggta
    32221 tattcccgca ccatgcgcgc ggtcgtaaat tgcggcacga gggtcaccac cgcgcggcgg
    32281 atgcgctgaa tccattggcg cgggatgccc tgcgcgtcgc gctggaagaa cagcgggatc
    32341 acgtcctgcg tcagcacctt gtaaagattg gcgctgtcca ccttgtcctg ctcctcgacc
    32401 gagctggcat gggcatcttc gccaatggca aagccgttgc tcccgtcgta gccctcgcgc
    32461 caccagccgt ccaggatgct caggttcagg ctgccgtggg tgccggattt catgccgctg
    32521 gtgccggagg cttccagcgg gcggcgcggc gtgttgagcc acacatcgca gccggacacc
    32581 atctgccggg ccacatgaat gtcgtagttc tccagaaaga ccaggtgccc cttgagatcg
    32641 ctgtatttgc tcaggtgaat cacatactga atgtagcgct tcccctcgtc atcgcgcggg
    32701 tgggctttgc cggcaaagat aaactggatc gggcgctccg ggtcgcgcgt gaggcgcaca
    32761 atattctcca actgctgaaa caccaggggc gcccgcttgt aggtcgcaaa ccggcgcgcg
    32821 aacccgatcg tcaacgcgtc gggattcagc aactggtcga acgcaatgaa atcgccgtgc
    32881 atcaggcgct gctgctgaat cagcagccgc cgccgcgcga actcgatcag ttcccggcgc
    32941 agcttataac gcaacgccca cagctcctcg tcggagataa actccgggtc cgcaacccgc
    33001 tgccagaact ccggggaatt gacctgcgcg gcccaatcgg cccccggccc gcgaaacccc
    33061 ggcggcgccg cgtcggcgag gctggctttg agcttgcgct gccagaaccg gcgcacggtc
    33121 cccttcatcc agcccagcag gtgaacccca ttggtgatat gcccgatcgg cacctcgtcc
    33181 accgaccggt ccggatacag gcagtgccac atctgccggc tgacccggcc gtgcaattcg
    33241 ctcacggcgt tggccgcccg cgacatcttc aacgccagca ccgtcatgca aaacggttcg
    33301 ttcgcatctt gcgggttcac ccggcccagc ttgagcacct ccgaaaacgg caccggcagt
    33361 tccgcccggt accgttgcat ggcgtagtcc accaggtccg ggcggaagcg gtcgtgcccc
    33421 gcttcgaccg gcgtgtgggt cgtaaaaata cactcggccc gggtctggct taacgcttcc
    33481 gcgaaactcc ggcccgccgc cagccgctcg cgcatcagct ccagcgtcaa aaaagccgcg
    33541 tgcccttcgt tcagatgata caccgacggc ttcactccca gggcgcgcag caggcgcacc
    33601 ccgccgatgc ccagcagcat ttcctgcatg atgcgggtgg tgctgtcgcc gccatagacg
    33661 cgctgggtca gctcgctgaa atgctgctcg ttctcgggac ggttggcatc aagcaaataa
    33721 accgtcgccc gccccacgtt cacccgccag gcctgaaacg ccacgtcgtt catcccgatc
    33781 cccacccggc acaccaccgg ctcgcccttc tcgttgagca ccggctcgat gggcaggttc
    33841 ttcggcttca actgcgtgta atactccgtc tgccagttgt tcgtatctat ggcctgctga
    33901 aaatagccct cccggtaaaa caagctgatc cccacaaagc ccacccccag gtcgctcgcc
    33961 gacttcgtat ggtctccggc caggattccc aacccgcccg ccgcaatcgg caaggcctcg
    34021 tgaaaaccga attccgccga gaaataagcc accgggttgg cccgcagggc cggggcgtgc
    34081 tcatgcgccc aggtgcgcga gtcattcatg taggtctcga aaagccgcag cacttcgttc
    34141 acccggtcgc agaaatccgg gtcttgcagg cgcacccgca gctcgtaatc cgacacctcg
    34201 cgcaggattg ccaccgcgtt gtggtagaga ttctgccagc cccgcggcga cagttcctga
    34261 aaaacgtcct gcgcctcctg gttccacgtc caccacaggt tccgcgccac gcggttcagc
    34321 gccacggtca attcctgcaa ttcctgagtt gtcagcggtt tcttggtcat aaaaactcta
    34381 tcagccaaat aagattctcg tttgccagta attcgcaacg ataccgtagt cgcccccgcc
    34441 gccaacggca attcaattcc cctcgcgctc gcgcgcccgg cggtgcaaac cagttccccc
    34501 gcgccgccgc cgggcgaatt gcttgaccca agtcaaggcg ccccggccaa aagccgtgca
    34561 atttatgcat ataaaacggc aaaaaacgtg tggttgaaac gagtggatta gccgagattc
    34621 ctgacgaaaa aatgaaacgg cagagcaagt ccgagttcag gtgccagcaa gagtgccgct
    34681 gcgaggtttg tccgctgagc cagattaagg ccggcgtcgc ggtgcgcatc aaacaattgt
    34741 gcggtgcgcc cgaactgcaa aaccgcctgc gcgaactggg cctgggcgaa gaccgaatca
    34801 tcaagctgct gaccagccag accaatttca tctgccaggt gtgcaacgcg cgcctggcca
    34861 tcagccagca attggcgcaa ttgattcttg tagaaccgct ccgccccgca gtcgtttaaa
    34921 gggaaggatc acgactcagg cagccgtcct ggacaaacgg ctgcctgagc gcctttaagg
    34981 agtggcggcc agacaaggcc ccccttgcat cttttcttgc ccgatggcca ggccaccagc
    35041 aaaaccactg attgaaacag tgccagcaga tagctgacaa cctctccctg cttagtcttc
    35101 ataccctccc atcaccaagc ggattttggc ggctcaaggt caagccggga actggcgtgg
    35161 catccagtaa aatggacgac gatgcaggtg catcacggct ttgacgtaga tcgcgccggc
    35221 gctgacgctg taaacgatgg cgcaggaaaa gcggtggaag ttgagcttgc gattgtcgcc
    35281 ccggattttg cgtcagcgtt ccggctcggc caggatgcgc cccagcgtag ctcgatattc
    35341 ctcaagaaag tcgctgccca gcttcggctg gcgttcctca taccaaagcg cggcggcttc
    35401 caactcttga ggaacttcag aatgccggat gacgcgcatg gagcttggcc tggatgtccc
    35461 ggatcatctc ttcctctggc cgggcatcca cccggccctc ggccatctcg cgggagcgcc
    35521 ggtcaatgac ttcgttccac tgcgtttcgg cgcccgcgtc aatggtgtcg tcaagactgg
    35581 cgatgagttg gcgagcccga tacgcgcggt cctgcccggg cagggccagc acttcggcca
    35641 ctttctcagc agcaatgctc acgcggccaa aatgtcggcc aagtgagccg ttctgacaag
    35701 tccgatagcg tttcataatt aaattaaggt tccccctcgc tctctcggat ggaacttgcg
    35761 gcgcgcttct ttcaagtcgg tgatggccag gcgcagataa cgctcggtcg tgttgagcga
    35821 gcgatgaccc aggatgtcct gccgtggcgc aggttggctt ttgggaagga ctggggacat
    35881 cgtgaccccc tggtggtgaa actcaaggtg ttgagccccc gacgcagaat ggaactgctg
    35941 cgcctcctcc ggaggaggcg cagccgcggc gcgaaaaatc tgccaccctc cgcgggggca
    36001 cagcacctac tttgaactct atcgtaggcg ccccgaccgt cattggtggc atcagaaact
    36061 ggttgcctgg gcccgcgcca acggcatcaa ggctgccgcc cgcgagttcg gcggctcccg
    36121 caaccccgtc cgcaaatggc ggcgccgtta tcaacccggt aaaccctcca cttgggtcga
    36181 acgcagccgt cgcccccaac actcgcccaa ccagatcccc aagggtctgg aaggccagat
    36241 cgtcaaactg cgcggcccaa ccggcttcgg cgcccaacgc ctccagcggg aatttcacct
    36301 gccttgccgc cacaacgccc ttgcccgggt ctttcgaacg catcaacggg cacggcctcg
    36361 gaaaaagaaa cccgccccca aacagcaact ccgcacggtc aaacgccact ggaaactctt
    36421 tggccagctc tccaccgaca cccaatatct ccaggatatc cctcatcact ggccgcaaat
    36481 gacccacctc aacctgcccc gcttccaata caccgtccgc gagcccgtct ccggcgcctg
    36541 ctttaccggt tatgccgatg aacggtccaa aagctatgcc accctcctgg ccgaacaact
    36601 ggcggtgccc ctggccggtc acggcgtgga cctctccggg gtggtcgggc aaaccgacaa
    36661 cggcagtgag ttcctggaaa atcaaccagc gcaagggctg cccaccgccg agtgcgcctt
    36721 ggggtgcgag caccgttaca ttccgcccca acgctaccct tggcaaagcg atgtcgaaac
    36781 ggtgccccga cgcgtcgaag atgagtgctt cgatcgggaa accttccgca gcccaacgga
    36841 cttctgggcc aaggtcatca cttactggct ctacttcaat ctggggcatc ccaatcgcgg
    36901 caaggaatgg cagagtccgt tgcaaatcct ccaacgccag gctccagccc tggttggagc
    36961 ggtcctggac tggcgaccgc ttgacctcgc caaacgtcac cacttctact tgcccaaacc
    37021 cctttaccag ggtcacgatg tccccagatt tccctttttc cttctctttt aacttgcctt
    37081 ttaaatccgg gcaggctata actagcttag ttgacgggac ttaataaaca ttcttgccta
    37141 tgactctgac aaaacgcgac ttagtgcttg gcatctgtga agagaccaag ctgccctacc
    37201 atcaggtctc caatgtggtt cagctcacct ttgatcatat catcaacgcc ctggcggcag
    37261 ggcggaaagt ggagttgcgc aactttggga tattcgaagt gcgtttgcga aaagcccgga
    37321 tcgggcgcaa tcccgccgcc cctgagtctc aagtgccgat ccctgaacgc tatggcgtca
    37381 agttcaaggc gggccgggaa atgcgcctgt cggtgcgcaa aatcgcgccg aaatccaacg
    37441 cctagccccc gtccccataa cgcgggacca aaggccagcc gttgccagtc ccggcaccag
    37501 gccagggggg cgcatcgtaa ggcgattgcc gtgcaagcgc cccctcaaga ccgtttccaa
    37561 caccattccg gcacgcctca gctaacaccc cgggaacggg gatgcccaac cgaggccagt
    37621 cctgttcgag gcgcctgaag ccgtgtcagc accggcggca accgactcat atccagaacc
    37681 gcgcaccccg cctgccgtac catcagccgc aaggag
//
LOCUS       DAOPQG010000026        34359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1661_length_34359_cov_15.550869, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000026 DAOPQG010000000
VERSION     DAOPQG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 34359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34359
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1661_length_34359_cov_15.550869"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PKI07_04560"
     CDS             <1..1179
                     /locus_tag="PKI07_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOC50356.1"
                     /translation="QYHHLQQPEDRKAVFEKYLGNFAEHRISPYSFYDYAPIDIRFVG
                     EGTNRQARVDFTQFDPAAERWLDQAHFSTFQLRLRGMGGGTFHSRHLGELEGFKEGTP
                     EFARLFQDYLSQIEAHLRARGWLSKAFTYWFDEPEPKDYEFVVDGMKRIHAAAPGIRR
                     MLTEEPHPQLFGHVDIWCGLTPQWTPELVRQRRQAGEEVWWYICCGPHAPYLTEFIDH
                     PGTELRLWPWQSWQYGVEGILIWATTYWTSATAFPPPKLQNPWADPMSYTTGYGLTPG
                     TINYWGNGDGRFLYPPRRDPVENQTPCLDGPINSLRWENLRDGMEDYEYLWLLQQAAQ
                     RELAWRGETELYQQAREALKVPAEISQDLTHFTTDPRLMLAHRDLVARLIERLQRQRR
                     "
     assembly_gap    1240..1339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1702..2754
                     /locus_tag="PKI07_04565"
     CDS             1702..2754
                     /locus_tag="PKI07_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50357.1"
                     /translation="MTRLAMVGVAFVALLIFYAWMTANRPAPTPDALPQEPGEEAGGR
                     QVVRRGGPPTKNQRPAEDAAAGQLSVQRVLKYGRGEGELGMVLGRGLAPTGPESFAVA
                     RDGSILVADVVNQRVVVYSRDGTFLRNIELPGIALGDIITDREGRVFIYDQVRRALHQ
                     YDTDGTLHDAFELNPKDIHIRGYFHAAGNAVFFADAAAQDVLVAVVRDGRLAPPDPST
                     ERRAPGIHSESDRVYALSLEKGQAARVQIWDAPEARGEGKALDIPWPGIVSARFVGED
                     DARRFYIQTERLEDDRVVLEVLAFSATGERLAVIQMPENDYAIWTAKLVDVRGDGALV
                     ALLPQQDQAKLNLFTY"
     gene            2805..6035
                     /locus_tag="PKI07_04570"
     CDS             2805..6035
                     /locus_tag="PKI07_04570"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC50358.1"
                     /translation="MKASEPRRQPAQTTLANPLAARARTYGGKRVACALIGLALLLRP
                     GVAAAWTGDPWGPLSRAAIQATADLMIDSTWTPKNTITNWEYGSTYHTFTKGVTYTGV
                     AYSQHNPQENWTEFYNYVTSTSGGTTYYGNDCSGFASICWRLPARKTTYLFESQLGTY
                     WISLGEKGSAATAPLLLSDALNSESDHIVLFLRRDPAGVATMEQTPYHAQRKVRSYSA
                     LSSYRPIRRLAITDAPVLTQAGLSRVVDEGEAVSLSVSASGTEPLSYRWRFNGAPLAG
                     ATTSQLTFPAAQLTNAGNYLCVVSNTSGSVTSRVMSLTVYPAQRTVFLDDFETNSAAR
                     WVVNRSSADTRVTFHYDYGAMGIPAAPHTAGGTTRGLRMEANLTAGVAAAVSLSPLGQ
                     QYRGDYRVRFDLWLNANGPFPEGGAGSTQHATAGVGTAGDRVEWGGSGSGADGYWFAV
                     DGEGQASDTSASSGDYCAYAGAALQSAGSGVYTAGTEANAKGNLHEYYVAAFPGGATA
                     PGAQQAAYAQQTGSLAGGTVGFAWREVMVARRGNEVDWAIDGVRLARLTNAVWGASNV
                     FVGYWDMFASLTDNTNVSFGVVDNVRVEVPVTEPVITEQPQSRALIAGENLVLRVGAM
                     GPGTLSYQWQRHGTNLAGATAAVLILPEVQPHQAGPYTVVVSNAYGSVLSQEAQVTVS
                     PVFAVDGWQSLWGLAPGSRPYLTTNSLPFERGMAYDPQARRLVLVSRVEPRVYVLDAE
                     TGADVGELGVSGISGGIYPLLMVGVADDGVVYAANLTTGGATTAFKLYRWASSAPGTE
                     PTLAYAGDPGAGNNQRWGDTLDVRGAGAGTQVILASRNGSVVAVLTTADGQTFSAKLV
                     TVAGVPNGAFGLGLGFGAGNTFWGKATGQNLRQVSFDLASGQGTVLRDYAAPLIPASL
                     APLGVSPALHLLAGIKVGSSGNQLQLYDLTPSNGVPVLVGVTNFMTDYDNNGSGTGAV
                     DFGGDRVYALCGNNGLVALQVQAAAVPWPPAEPGRFEAIHRLPDGAVQLTMSGTAHTN
                     YLLQWTADWLTWSNLGALESGEGRFWLLDSSATNSARRFYRLQLAP"
     gene            6053..9928
                     /locus_tag="PKI07_04575"
     CDS             6053..9928
                     /locus_tag="PKI07_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC50359.1"
                     /translation="MMKTNQTLLSLTLRMAAGLALLAAAAGFESAARAATSLVYGIVT
                     NPGEDCSHEMNISWHAQLGSTNCYVTYTKKTDTAWANATTVYGTNAYCDRFDGIYSKT
                     ASGADFYESAIFLNYGAVLSGLTPDTEYMYQVCASPGACSTTHYFKTAGASEFSFIWI
                     SDFHAYPPLSGRLSNAIRAINAAVVIDPSVDFIFSTGDVVAWGGSYSFWTNLYAQNFI
                     RDYMFANVVGNHDYMTRSYALSSDFFRIANNFPLNGYAGQWGVCYWFIYQNVLFLTLN
                     NEVMNGNTAAQTAAKNWAASVIQSQRGRYQYIFLAEHYQWFYGQSGQTSWYANWKEFC
                     DQYGVDLALSGNNHIYVRTHPLYNDAVVPNGQGTVYMEAPSSDGERGVEAGTITYNAE
                     KIAYTYSSHTPSGGGQVKTIGCVLVKVNAQGITTKLVYLDDNKVAHVADENTITAAPV
                     LTQAGLSRVVDEGEAVSLSVSASGTEPLSYRWRFNGAPLAGATTSQLTFPAAQLTNAG
                     NYLCVVSNTSGSVTSRVMSLTVYPAQRTVFLDDFETNSAARWVVNRSSADTRVTFHYD
                     YGAMGIPAAPHTAGGTTRGLRMEANLTAGVAAAVSLSPLGQQYRGDYRVRFDLWLNAN
                     GPFPEGGAGSTQHATAGVGTAGDRVEWGGSGSGADGYWFAVDGEGQASDTSASSGDYC
                     AYAGAALQSAGSGVYTAGTEANAKGNLHEYYVAAFPGGATAPGAQQAAYAQQTGSLAG
                     GTVGFAWREVMVARRGNEVDWAIDGVRLARLTNAVWGASNVFVGYWDMFASLTDNTNV
                     SFGVVDNVRVEVPVTEPVITEQPQSRALIAGENLVLRVGAMGPGTLSYQWQRHGTNLA
                     GATAAVLILPEVQPHQAGPYTVVVSNAYGSVLSQEAQVTVSPVFAVDGWQSLWGLAPG
                     SRPYLTTNSLPFERGMAYDPQARRLVLVSRVEPRVYVLDAETGADVGELGVSGISGGI
                     YPLLMVGVADDGVVYAANLTTGGATTAFKLYRWASSAPGTEPTLAYAGDPGAGNNQRW
                     GDTLDVRGAGAGTQVILASRNGSVVAVLTTADGQTFSAKLVTVAGVPNGAFGLGLGFG
                     AGNTFWGKATGQNLRQVSFDLASGQGTVLRDYAAPLIPASLAPLGVSPALHLLAGIKV
                     GSSGNQLQLYDLTPSNGVPVLVGVTNFMTDYDNNGSGTGAVDFGGDRVYALCGNNGLV
                     ALQVQAAAVPWPPAEPGRFEAIHRLPDGAVQLTMSGTAHTNYLLQWTADWLTWSNLGM
                     LSSPNGLFAWVDGSASNAPRRFYRLQLGP"
     gene            10032..11120
                     /gene="mnmA"
                     /locus_tag="PKI07_04580"
     CDS             10032..11120
                     /gene="mnmA"
                     /locus_tag="PKI07_04580"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOC50360.1"
                     /translation="MAAVKKERVVIGLSGGVDSSAAAALLVEAGYDVVGITLKLWAHG
                     RWLPGTGVDRREQAVADARAVCQRLGIPHYTLDESAEFERCVVQYFADEYKAGRTPNP
                     CARCNLHLKFGRLLQQAEKLGAQWVATGHYARRERSPDGARLLLKRGRDLRRDQSYFL
                     FALRQDQLARALFPLGEKTKTAARAQARDRELQTADREDSMEICFVPEADYRTFLQQG
                     GFAPKRRGEIVDLQGRVLGEHDGVAFYTIGQRRGLGISSTRPLYVIELDAARNRVIVG
                     DNSALMGSQFVAERCNWIAFERPPARLEATVKLRYNHAGAPATLTPRGDGSAEVRLHV
                     PQRAITPGQAAVFYHDDLVLGGGWITRS"
     gene            11484..12206
                     /gene="truB"
                     /locus_tag="PKI07_04585"
     CDS             11484..12206
                     /gene="truB"
                     /locus_tag="PKI07_04585"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOC50361.1"
                     /translation="MHEFTALDGAILVDKPVGLTSHDVVDAIRRHFRIKKVGHCGTLD
                     PNATGLLIIVLGRGTKLSERLMSDDKAYEGVIKLGETTSSHDADGELTASLPVPPITL
                     EQLNATAAEFVGDLMQTPPMVSAVKKDGVPLYKLARKGIEVPREPRLVHIYTFRFSAY
                     QEPLAQFRLVCTKGTYVRSLAHDLGQKLGCGGHLASLRRVASGKFEVSGAIPLAEVLK
                     LTPRELEKRVLPFLKLAGAAAS"
     gene            12242..13165
                     /locus_tag="PKI07_04590"
     CDS             12242..13165
                     /locus_tag="PKI07_04590"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOC50362.1"
                     /translation="MRIIHAASELNPAGRKVCLGIGFFDGVHLGHQQIIRQTLTDARQ
                     HDAVGLVVTFDPHPSVIVAPDRVPPLIYSPSRKLRAIEALGADTLLLIRFDRAFSEQT
                     GEAFVRKLARDLGRLQSLCVGANFVFGHQRRGNVELLRKLGGELGFTVHGMAAVALGG
                     RTVSSTRIREAIRSGDLDQAGQMLGRAYSLTGPVVRGDGLGRQLGFPTANLETAGLAL
                     PPHGVYAAWAQTGNLTRRAVLSIGLRPTLPNPRPQLRVEAHLLDFSGDLYGRELEVVF
                     VERLRAEEKFASLADLQRQIARDILQAQTRF"
     gene            complement(13162..14928)
                     /locus_tag="PKI07_04595"
     CDS             complement(13162..14928)
                     /locus_tag="PKI07_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOC50363.1"
                     /translation="MKSSPDISLSTALRPTVEVLLPDGRILCGPRNQPISAFLKRLPE
                     WSSNDPIVGAVVNGELRELTFPIELDSRVSLVRMSSPDGSGIYRRSITFLLEAAFADV
                     FPAADISIDHSLSSGGFYCKVINRPPLSREELAAVEARMKELVKADLPFERQVVPLRE
                     AMDYFQSKGQDEKVQLLRFRRKDTLVLYKLGGRCDYHHGFMVPSTGYLRWLALTPVGE
                     GFALRYPRRHAPNELRPLPDSKKLLATFLEYNDWLVRLGIENVGALNAAIESKRASEL
                     ILVSEALHEHRIAEIARLVMARAHEARIVLVAGPSSSGKTTFSKRLAVQLLAQGISPF
                     PLELDNYFLDREKTPRDEKGAYEFEALGALDITRLLSDLKQLVAGQEVQLPRFNFPLG
                     RSQPGDVVKLEKGQLVILEGIHGLNPHLLPGLAPGQTTRIYVSCLTQLNLDRHNRIST
                     TDTRLLRRIIRDARDRGYSAQQTISHWESVGRGEKKHIFPYQENADVMFNSALVYELS
                     ALRMLAEPLLRQVVFGTPEYIEAKRLLSFLQWFLPLGPELIPDNSLLREFIGGSILKE
                     FKLWRNGRSNGESDFPPPAPAG"
     gene            complement(15591..16481)
                     /locus_tag="PKI07_04600"
     CDS             complement(15591..16481)
                     /locus_tag="PKI07_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOC50364.1"
                     /translation="MKYVFQLVLGIACAAHAGAAGSGIIAPGATLRKLAGEFAFTEGP
                     ASDRKGNVFFTDQPNDRILKWSVDGRLSTFMQPCGRANGLSFDRKGRLWACADAQNEL
                     WRIARAGKPAVILKGYQGQRFNGPNDLWIHPRGGLYFTDPYYHRSYWKRGPKEMGEHV
                     YYLAPDCQTLTRVIDDLQQPNGIIGTPDGKWLYVTDIKAGKTYRYHIQPDGALTGKKL
                     FCELGSDGMTLDHRGNLYLTGKGVSVFNPDGLKIEQIEVPEPWTANVCFGGKGRKTLF
                     ITASKSLYALPMAVHGVDSQ"
     gene            16914..19574
                     /locus_tag="PKI07_04605"
     CDS             16914..19574
                     /locus_tag="PKI07_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOC50365.1"
                     /translation="MKITYPALIRPAVRGWALTLAVAGGLLAFAGTTQAQPTQVYYED
                     FEVDNTQNWIVNYGLGANVADFYFDYSSVGIPPAPHTVGGTTRGLKLQANVDPNTQAG
                     SVAGYGLSVSPIGFSITENFEMRFDMWLNYNASGLGSTQIGGAGFGTAGTQAQRAMPI
                     PGVVDSIFIGASTDGGTAADYRVYSPVHYIGLQDASGVYAAGSRNNTATYYVTNFPGN
                     VAPPAAQTNLFPSQASLRTPDGTVGMQWRDVALRKVANIITYRIDGVLIATIDASTNG
                     TLGGANILFNFYDIQGNASTDPLATNLLFALFDNVRITNFPSVVTVSATTPDAAESGP
                     TPGIFTLTRSEPGPEVTVFYEMSGTASNGIDYVTLPGSITFAAGATSTNITLTPIDDN
                     LSEFAETATLTISEGPNYYGAGSATVTIADNDTPTIDISTVLGSMYEPLPHDYVRFRL
                     TRRGDLQAPSFNVNLVYSGEAAATRHTGTTPVTVDPGSLYTDFDVNPIDDHLLQGNQT
                     IVCTVTSGTGYAVGTTSPSATATIVDDELPAETILFADDFNTDTSANWMLLYASTNAA
                     DNDYSAIFNYDYSQHPYGPMIPPAPRSGTDTHGLFLQVNKGDATEAAAALNLYPRNQN
                     FTGNFAVRFDMYLIAPDTATTEYALFGINHSGNQTNWFRNSAAGFAGVDPVGWTFDGL
                     FYGVETDAAALGDYVLYSAPTTAGRNPTALNSRSASTLTGIFKKPPYGYAGSPGNLSG
                     SSTPCWSEVEISQVGNLVTLKINQTEIFSYTNNTAFTSGNIMIGHCDAYDSAATYDGG
                     VIYDNVRVIRLPTSLAPATITSIQPVGGPPTGYLVNYTGGSGSQFVLLTSPTVTAPLS
                     SWTRAATNSTGSGTFSVPAGAQAFYRIKSE"
     gene            complement(19762..21156)
                     /locus_tag="PKI07_04610"
     CDS             complement(19762..21156)
                     /locus_tag="PKI07_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50366.1"
                     /translation="MRIPVSACGVLLLAGLAWPPLALRAVEPCRIEVIEHGTGWPVPM
                     VELRTTHSVRFVTDNAGVIAFDLPELMGRETWFDVHGQGYERPKDGFGYRGARLAPQP
                     GQTLKIEVNRTIIARRLGRITGGGLFAESQKCGRELTWRESGILGCDSVQNAVHRGKL
                     FWIWGDTIVPQYPLGIFDTTCATSAIQPLAGFAPPLRLKLDYFADTNGVPRAVARLPG
                     SGPTWLSGCVSLPDKHGTPRFVSTYTKIKPRMEAYECGLCVWNDDRQLFEPHRVLWTK
                     SDSQPKPTLAPWGQPAFWTNAHGQQTWVLFGNPLPIMRCRASFEAWQDPSQWQALEPQ
                     SAFTSATDGAAVKPHSGHIAWNPWRRRWVTIFMESFGKPSAMGELWYAEADAPTGPWG
                     KAVKVLSHDNYSFYNPRLHPEFTPADSPILIFEGTYTTEFAGRPSPTPRHDYNQVLYR
                     LDLDDPRLSPAQER"
     gene            complement(21153..21551)
                     /gene="acpS"
                     /locus_tag="PKI07_04615"
     CDS             complement(21153..21551)
                     /gene="acpS"
                     /locus_tag="PKI07_04615"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOC50367.1"
                     /translation="MGIDIIEVSRIQASHEKFGERFLNRILHPNEIAYCLAHRAPAPF
                     LAARFAAKEAISKAFGTGIGAQLGWRDMEIGRHASGQPFVILHEAGRQLLAARGGQRL
                     QISLSHTQNYAAAVAVLEGQESTSSPPADV"
     gene            complement(21621..22361)
                     /locus_tag="PKI07_04620"
     CDS             complement(21621..22361)
                     /locus_tag="PKI07_04620"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOC50368.1"
                     /translation="MLKLGVNVDHVATLREARYRGRERGEPDPVAAALACEAAGAHGI
                     TVHLREDRRHMQDRDVWKMREAIKTRLNLEMAVAPEIVAIALRLKPDIVCLVPERREE
                     ITTEGGLEVAGNLAVITETRKRMNDAGIEVSLFIAPDPAQIEASARAGSQFIELHTGA
                     FAEAFDQEAHRQAELERLIAGARQAHALGLNVNAGHGLNYQNLQMLHQVPHLVELNIG
                     HSIISRAVMTGLTTAVQDMLRLMAGYPG"
     gene            22451..22909
                     /locus_tag="PKI07_04625"
     CDS             22451..22909
                     /locus_tag="PKI07_04625"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOC50369.1"
                     /translation="MPRKQLFIARWLLVLCWMGVIFSASGDQMSFDRSRHIIGSFLCW
                     LVPQLSPEAIHAVVVVLRKCAHVAEYAVLAWLIWWALDQFSVSRAPDWPWPKAGLVLT
                     LVTLYAISDELHQTFIPSRGGCVQDVLLDTLGGALGLLVLWAACRLKKRE"
     gene            complement(22954..23781)
                     /locus_tag="PKI07_04630"
     CDS             complement(22954..23781)
                     /locus_tag="PKI07_04630"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOC50370.1"
                     /translation="MKKQLALAAKRVGIAFLLVVAGAGAEESAVAGSPLSPPPGKPSR
                     QTYRVRWRPGAQITLDGRADEPAWMQAVVNRRFAFPWKRAKAPKTEFRALCDGMHLFF
                     TFRVQDADTVVLDHLRDELDVVLEDRVEVFLGRDDQMQDYYCFEVDARGRVYDYRGSY
                     YRRLEAKWQCMGLETRGVLLPGGYEVEGRIPLESLVALGFPALRPGVKIRGGLYRAEF
                     SHDRSGRPIEPQTSVHNLGRKAEGPPPVEEWISWVDPKTRDPDFHVPASLGWLKVVR"
     gene            24219..24374
                     /locus_tag="PKI07_04635"
     CDS             24219..24374
                     /locus_tag="PKI07_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HOC50371.1"
                     /translation="MSQHRSLRAAATLGGKRNVLKRFERIELLKKRGQWKEGDRITGL
                     RKTKKDA"
     gene            24435..25139
                     /locus_tag="PKI07_04640"
     CDS             24435..25139
                     /locus_tag="PKI07_04640"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOC50372.1"
                     /translation="MVRLQKFLADAGIASRRVGEQMILAGRVAVNGQTAGELGFRIDP
                     ARDRVAVDGKPIRAKRKVYVALNKPRGVVCSRRDEFERPTVYSLLPGEWSHLHTVGRL
                     DYHSEGLIFLTNDGEFSLRLTHPRYGVRKRYVATVEGRVAEEVLGQFTLGIWHQGEKL
                     KAERTRLVSSGSTQSVVELELAQGKNREVRRLFESQGMTVKRLQRIQIGKIKLGELRP
                     GKWRILTEPEIKSLMC"
     gene            25335..26369
                     /locus_tag="PKI07_04645"
     CDS             25335..26369
                     /locus_tag="PKI07_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOC50373.1"
                     /translation="MAHKAPVKKAPARKTATWPELRTMPLVAGPAMVVASNVNVRGQA
                     KLKSEIVTQVTKGQTVTVIEEIVRNNSGPDEPSAWAKITLPPGARAWVHNAYLKAADQ
                     TVVPRRLNLRSGPGENYSVIGLLHRGDVVKPIITKEDWTEIEPPAIAYAFVAAQYLSQ
                     EALPPSVPTPTAVTAVPPVAPPPEPPPAPATVTVIPPGPSQPVEPPPTPTTVAADPTV
                     APPPAEPPALPPPTATPDVTTLAETPAAPSEPEMIETPEEEEEPLPPRIVEREGIVRG
                     MTSIQAPSPFVLVSPETGRNIDYLYTTSRQLDLRRYKGLRIIVTGEEALDERWGTTPV
                     ITIQKIQVVE"
     gene            26369..27256
                     /locus_tag="PKI07_04650"
     CDS             26369..27256
                     /locus_tag="PKI07_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOC50374.1"
                     /translation="MAPANLIDGRAIARQLQGELVQRIEALKASGVQPGLAFVRVGED
                     AASKVYVGRKEKMCAELGIASETHVLPEQTSEPELQVLLDRLNADRQLHGILVQAPLP
                     PHLRATTVYARVSPEKDVDGFHPLNVGKLMLGDPTGFLPCTPAGIRELLARSGVQVAR
                     AEVVVLGRGNIVGKPMAAMLCQKAPHANATVTLCHSATRDIGAHCRRADILIAAMGVA
                     EFVKADMVKPGAVVVDVGVNRVSDPSAKGGSRLVGDVDFAAVQPIAGQITPNPGGVGP
                     MTIAMLMQNTVRAAEHAAR"
     gene            27270..27713
                     /locus_tag="PKI07_04655"
     CDS             27270..27713
                     /locus_tag="PKI07_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50375.1"
                     /translation="MQPTRILPDLQCSLMCEEVRQEANANFFLIGVLGLIRVPQLPVM
                     AFKLSVFNRWTAGVGQFIETTRFIAPDQTTVLRKGEVKFSLKEAGQHATNVTVFTQVE
                     FKTVGTYYVEVLVDDVMKLRYPVPVIHTPPPQPPSAPPVPPAPAS"
     gene            27788..28594
                     /locus_tag="PKI07_04660"
     CDS             27788..28594
                     /locus_tag="PKI07_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50376.1"
                     /translation="MNAPDAKTQAAWQPLTFKGVAAFAGGTMSQLLLIQIVVALLAGS
                     TVIWLVHRAWFPTIEEAINSLPPQGELRSGQLLWTGDASARLAEGRFLALTVDLDHAG
                     KVRSPAHIQIEFGRTDFRVYSLLGSCQGSYPRGQSLAFNQTELRPWWGAWAPAILMIT
                     GLGVAAGLLVIWAGLATLYTLPVWLAGIFADRQCSLGGSWRLAGAALMPGALLTCAAI
                     LLYGGGRLDLLQLAAIGVAHVMLGWVYLILSLLRLPRLEEAEAVRRNPFA"
     gene            28629..29399
                     /locus_tag="PKI07_04665"
     CDS             28629..29399
                     /locus_tag="PKI07_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC50377.1"
                     /translation="MMFSVGAYRSALLALACLLLSGCLPSAPGEEEKEPHFLAGRNRV
                     NSLDFRGAIESFEKALEANPRSASAHFELGWLFDQKEPDPAAAIYHYERYLKLFPNSG
                     KEELVKTRILACKQQLAQAISLAPGMEKLQRDLEQLTTENKRLREELEKWRASASRLQ
                     SSAASPTPAIPGPREVQLPSPSAAVTTGAALPTGGSPNRLPSVPPAPARTHTVKKGEN
                     PTTIARIYGVKVESLLAANPRLNPRRMQVGQVLKVPPR"
     gene            complement(29521..30426)
                     /locus_tag="PKI07_04670"
     CDS             complement(29521..30426)
                     /locus_tag="PKI07_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC50378.1"
                     /translation="MHAMKNVLGACLLLLGASVGAQTNDNFTRTEDVIYGRKFGTALT
                     LDVIQPQPANGVGILHMVSGGWVSAHEFINANYLKPLLKRGYTVFAVVHGSQPKFVIT
                     EIVPDIHRAVRFIRHNAARYGVDPNRLGITGGSAGGHLSLTMATQGGPGDPQARDPVD
                     RESSAVQGVACFFPPTDFLNYSRPGEDAVGVGVLKDFKAAFGPRADTAEERQKLGREI
                     SPIYFVHSNMPPVLIIHGDADKLVPIYQSQTFVQRCREEGVIARLEVREGKQHGWPDS
                     SRDIELFADWFDEHLRGLKPKPRGN"
     gene            complement(30521..31630)
                     /gene="ychF"
                     /locus_tag="PKI07_04675"
     CDS             complement(30521..31630)
                     /gene="ychF"
                     /locus_tag="PKI07_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOC50379.1"
                     /translation="MLKAGIIGLPNVGKSTLFNAITRSHKAPAENYPFCTIEPNLGVV
                     SVPDPRLDQLAQVVKVGTRIPTTFEFVDIAGLVKGAAHGEGLGNKFLSHIREVDALVQ
                     VVRCFEDPDIVHVAGDIDPVRDIEIVTAELVLADLETVRKRLEKIAREVKRGDKHALV
                     EAHLLEKLGDHLNTGQPANTLGLPLAPSEKIIVHGFFLLTDKPTIYVANVTESDLAAV
                     DTHPQVAKVRQYARAHQACDTVVVCAQLESEVADLSPEEAQDYLRELGVREPGGAALI
                     QSTYHLLGLRTFFTFNQKEVRAWTIPAGATAIKAAGTVHSDFERGFIKAETVNWADLV
                     KEGSVSRARETGHYRLEGRDYVVRDGDVLLFKFNV"
     gene            complement(31743..32903)
                     /gene="obgE"
                     /locus_tag="PKI07_04680"
     CDS             complement(31743..32903)
                     /gene="obgE"
                     /locus_tag="PKI07_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOC50380.1"
                     /translation="MFIDEIKVYARAGHGGKGCVAFQREKFRPKGGPSGGNGGRGGDV
                     ILEADHDLNNLIAQFYQPRLLAADGEPGLGKGMDGRAGRDLVVKVPCGTLVWRLDAAA
                     DGEISRTSARREYQSQSRRRRMLRTLPGEADADGEEAALPVSVPAAGGLASGPESRGE
                     LVIDLVEPGQRFVLCQGGRGGLGNRNFATAVRQTPRFAQPGEPGGEGNFLLELRIMAE
                     VGLVGYPNAGKSTLLTAISRARPKIAPYPFTTLHPQIGIVEYPDYHRLTVCDVPGLIE
                     GAHRNVGLGHEFLRHIERCKVLVLLLDMAGTDGRAPWDDYRSLLRELELYDPALVEKP
                     RLIVANKMDEAAARENLKAFKCRIRRVSILSMAAAFGEGLAEFKQAIRAAAG"
     gene            complement(33085..33348)
                     /gene="rpmA"
                     /locus_tag="PKI07_04685"
     CDS             complement(33085..33348)
                     /gene="rpmA"
                     /locus_tag="PKI07_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOC50381.1"
                     /translation="MAHKKGQGSARNGRDGISKRLGVKRFGGERVSAGSILVRQRGTK
                     FVAGRNVGTGRDWTLFALVDGRVAFDRNSRRVNVLAEAAPTGS"
     gene            complement(33394..33705)
                     /gene="rplU"
                     /locus_tag="PKI07_04690"
     CDS             complement(33394..33705)
                     /gene="rplU"
                     /locus_tag="PKI07_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOC50382.1"
                     /translation="MYAVLETGSKQYRVSAGDTLEVDRLAVDAGQAVNFDRVLLVNND
                     GKVTVGAPTVTGATVTAEVVKHIRGDKTLVYKMKRRKGYRKTIGHRQELTVVKVKEIK
                     L"
BASE COUNT         6429 a        10385 c        11178 g         6267 t
ORIGIN      
        1 cagtaccacc acctccagca gccggaagac cggaaggcgg tcttcgaaaa atacctgggc
       61 aacttcgccg aacaccgcat cagcccctat tccttctacg attacgcgcc cattgacatt
      121 cgttttgtcg gagagggaac caaccggcag gcgcgggtgg acttcacgca attcgacccg
      181 gcggcggaac gctggctgga ccaggcgcat ttcagcacgt tccaactgcg cctgcgcggg
      241 atgggcggcg gcacgttcca cagccggcac ctgggcgaac tggagggttt caaggaaggc
      301 acgccggagt ttgcgcgctt gttccaggat tacctcagcc aaatcgaggc gcacctgcgg
      361 gcgcggggct ggctgtccaa ggccttcacc tactggttcg atgaaccgga acccaaggat
      421 tacgagtttg tggtggacgg aatgaagcgc attcatgcgg cggcgccggg aatccggcgc
      481 atgctgacgg aagaacccca cccgcaattg ttcgggcatg tggacatctg gtgcggactg
      541 acgccgcaat ggactcccga actggtgcgc cagcggcggc aggccggcga ggaggtgtgg
      601 tggtatatct gctgcggccc gcacgcgccc tacctcaccg agtttattga ccaccccggc
      661 acggaactgc ggctgtggcc ctggcaatcg tggcagtacg gcgtggaggg catcctgatc
      721 tgggcaacca cttactggac cagtgctacg gccttcccgc cgcccaaact gcagaacccc
      781 tgggccgatc ccatgagcta caccacgggc tacggcctga cacccggcac catcaactac
      841 tggggcaacg gcgatggacg gttcttgtac ccgccgcggc gggatccggt cgaaaaccaa
      901 accccgtgcc tggacggccc gatcaactcg ctgcgctggg aaaacctgcg cgacggaatg
      961 gaagactacg aatacctgtg gttgctgcaa caggccgcgc agcgggaact ggcctggcgc
     1021 ggcgaaaccg aattgtacca acaggcgcgc gaggcgctga aagtgccggc tgagatttcc
     1081 caggacctga cgcatttcac caccgacccc cgcctgatgc tggcgcaccg cgacctcgtg
     1141 gcgcggctga tcgaacggct gcagcggcag cgaaggtagg cgcgcgttcc cccgcgcctc
     1201 cccgccaagc cgggcggcgc gggcttgctc ctggccccgn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnc tatgagcacc cgatgagcat cccttgtaca ccccttgtac
     1381 acggcgcttt ggcaggggtg cgggatgggg attgggagca ttagtttcgt gcaatttccg
     1441 gtgtaaatac ggaaagaatt gagcttgcct ttaggatgcg aaggggtagc atggcaacgt
     1501 gttgaggcga gggtgcggtt cgcattggcg atgaaagccg cactttggcc gaaacaagat
     1561 tatgtggcta aagctacacg tagtgcgctg ccaggcagcc gaaaacccgg aaccgggctg
     1621 ctgctttcct gtcgcctgcc gcggcggaaa tgagggtggc ctgaagccat gagcacgacg
     1681 cctcctgccc ggaaaccggc tatgacgcgc ctggctatgg tgggggtggc ctttgtcgcc
     1741 ctgcttatct tctatgcctg gatgactgcc aaccgccccg ctcccacgcc agacgccctt
     1801 ccgcaggaac ccggcgagga agcgggaggg cggcaggtgg ttcgccgggg cgggccgccg
     1861 acgaagaacc agcgacccgc cgaagacgcg gcggcggggc aactcagtgt tcaacgggtc
     1921 ctgaaatatg gccggggcga gggggagctg ggcatggtac tgggacgggg actggcgccg
     1981 acggggccgg aatcgtttgc ggtggcccgg gatgggagca ttctggtggc ggatgtggtc
     2041 aaccagcggg tcgtggtcta ttcccgcgat ggaacctttc tgcgcaacat cgagctcccg
     2101 ggcatcgccc tgggggacat catcacggat cgggaaggcc gggtgtttat ttatgaccag
     2161 gtgcgccgcg cgcttcatca gtatgacact gacggcaccc tgcacgacgc gttcgagttg
     2221 aacccgaaag acatccacat ccgcggatac tttcacgcag ccggcaacgc ggttttcttt
     2281 gccgatgccg ccgcgcagga tgtcctcgtg gcggtcgtgc gggatggccg gctggcgcct
     2341 cccgacccgt ccaccgagcg ccgggcgccc ggcatccact cggagagtga tcgcgtctat
     2401 gcgttgtcgc tggaaaaagg gcaggccgcg cgcgtgcaga tttgggatgc gcccgaggcc
     2461 cggggcgagg gcaaagcgct cgacatcccc tggccgggga ttgtgtccgc ccgatttgtg
     2521 ggcgaagacg acgcacgccg attttatatc cagacggaac ggctggagga cgaccgggtg
     2581 gttctggagg tgctggcctt cagcgccacg ggggaacggt tggcggtcat tcagatgccg
     2641 gagaacgatt acgccatctg gacggccaag ctggtggatg tgcggggaga cggggcgctg
     2701 gtggcgctcc tgccgcagca agatcaagcc aagttgaacc tttttacgta ttaacgaaga
     2761 tacccatgat gactctgccc atgaaatcgc accgggaacc ttttatgaaa gcatctgaac
     2821 cgcggcgcca gccggcgcaa acaactttag ccaacccgct tgcggcgcga gcgcggacgt
     2881 acggcggcaa gagggttgcc tgcgcgctca tcgggctggc gctgctgctg cggccgggcg
     2941 tagcggcggc gtggacgggc gatccatggg gtccgctgag ccgcgcggcc atccaggcca
     3001 ccgcggacct gatgattgac tcgacctgga ctcccaaaaa caccatcacc aactgggagt
     3061 atggcagcac ctaccatacc tttaccaagg gggtcactta caccggcgtc gcctacagcc
     3121 agcacaatcc gcaggaaaac tggacggaat tttacaacta cgtcacctcc accagcggcg
     3181 gcaccaccta ttatggcaac gattgctcgg gcttcgccag catctgctgg cggttgccgg
     3241 cgcgcaagac cacctatctc ttcgagagcc agctcggcac ttactggatc agcctggggg
     3301 agaaggggtc cgccgccacg gcgcccctgt tgctgagcga cgcgctgaat tccgagtccg
     3361 accacatcgt gctgtttctg cggcgtgacc ccgccggggt cgccaccatg gagcagacgc
     3421 cctaccatgc ccagcgcaag gtgcgttcct acagcgcgct gagctcttac cggccgatcc
     3481 gccggctcgc catcacggat gcgcccgtgc tgacgcaggc gggtttaagc cgggtggtgg
     3541 acgaggggga agcggtgagt ttgagcgtca gcgccagcgg gaccgagccg ctgagctacc
     3601 gctggcggtt taacggggcg ccgctggcgg gggcgaccac gagtcagttg acgtttcccg
     3661 ccgcgcaact gaccaacgcg ggcaattacc tgtgcgtggt cagcaatacc tccggcagcg
     3721 tgaccagccg ggtgatgtcg ctgacggtgt atccggcgca gcggacggtg tttctggatg
     3781 actttgagac caacagcgcc gcgcgctggg tggtcaaccg gagttcggcc gacacccggg
     3841 tgacgtttca ctacgattac ggggcgatgg ggattccggc cgcgccgcat acggcggggg
     3901 gcacgacgcg ggggttgcgg atggaggcga acctgacggc cggggtggcg gcggcggtgt
     3961 cgttgtcgcc gctgggacag cagtaccgcg gggattaccg ggtgcggttt gatttgtggc
     4021 tcaacgccaa cgggccgttt ccggaggggg gcgcgggctc gacgcagcac gcgacggcgg
     4081 gggtggggac ggcgggcgac cgggtggagt ggggcggcag cgggagcggg gccgacgggt
     4141 attggtttgc ggtggacggg gagggtcagg ccagtgacac ctccgccagc tcgggggatt
     4201 actgcgcgta tgcgggggcg gcgctgcaga gcgcgggcag cggggtttat acggcgggca
     4261 ccgaggccaa tgccaagggg aacctgcatg agtattacgt ggcggcgttt ccgggcgggg
     4321 cgaccgcgcc gggggcgcag caggcggcgt atgcgcagca gacgggaagc ttggcggggg
     4381 gcacggtggg gtttgcgtgg cgggaggtga tggtggcgcg gcggggcaac gaggtggact
     4441 gggcgattga cggggtgcgc ctggcgcggc tgaccaacgc ggtctggggg gcgagcaatg
     4501 tttttgtggg gtattgggac atgtttgcgt cgctgacgga taatacgaat gtgagctttg
     4561 gggtggtgga caacgtgcgg gtggaggtgc cggtgacgga gccggtgatc acggagcagc
     4621 cgcagtcgcg ggcactgatt gcgggggaga acctggtgtt gcgggtgggg gcgatggggc
     4681 cggggacgtt gagctatcaa tggcagcggc acggcacgaa cctggcgggc gccacggcgg
     4741 cggtgttgat tttgccggag gtgcagccgc accaggcggg gccctacacg gtggtggtga
     4801 gcaatgctta cgggtcggtg ctcagccagg aggcgcaggt gacggtgagc ccggtttttg
     4861 cggtggacgg ctggcagtcg ttatggggcc tggcgccggg gtcgcggccg tatctgacga
     4921 ccaacagcct gcccttcgag cgggggatgg cttatgaccc gcaggcgcgg cgcctggtgt
     4981 tggtgagccg cgtggagccg cgggtgtatg tgctggacgc ggagacgggc gcggatgtgg
     5041 gggagttggg ggttagcggg ataagcggcg gcatttaccc gttgctgatg gtgggggtgg
     5101 cggatgatgg ggtggtgtat gcggccaacc tgacgacggg cggggcgacg accgccttta
     5161 agctttatcg ctgggccagc agcgcgccgg ggacggagcc gacgctggcg tatgcgggcg
     5221 atcccggggc gggcaacaat cagcgctggg gggacacgct ggacgtgcgg ggggcggggg
     5281 ccggcacgca agtgattctg gcctcgcgga acggcagcgt ggtggcggtg ctgaccacgg
     5341 cggacgggca gaccttcagc gcgaagctgg tgacggtggc gggggtgccg aatggcgcgt
     5401 ttggattggg gctggggttt ggggcgggca acacgttttg gggcaaggcg accgggcaga
     5461 atctgcggca ggtatcgttt gatttggcga gcggccaggg gacggttctg cgcgattacg
     5521 cggcgccgtt gattcccgcc agcctggcgc cgctgggggt gagcccggcg ttgcatttgc
     5581 tggcggggat caaggtgggg agcagcggca atcagctcca gctttatgat ctgacgccga
     5641 gcaatggcgt tccggtgttg gtgggggtga ccaactttat gacggattac gacaacaacg
     5701 gcagcggcac gggggcggtg gattttgggg gggatcgggt ttatgcgtta tgcgggaata
     5761 acgggctggt ggcgctgcag gttcaggcgg cggcggttcc gtggccgccg gcggagccgg
     5821 gacgttttga agcgatccac cggctgccgg acggagcggt gcagttgacc atgagcggca
     5881 cggcgcacac caactacctg ctccaatgga cggccgactg gctaacctgg tccaacctcg
     5941 gcgcgctgga gagcggcgaa ggccggttct ggctgttgga ttcgagcgcg acgaacagcg
     6001 cccggcggtt ttaccgtctg caattagctc cctaaacctt taaccctgag aaatgatgaa
     6061 aactaaccag acccttttga gcctgaccct ccggatggcg gccggcctgg ctttgctggc
     6121 cgccgcggct ggatttgaat cggcggcgcg cgcggcgacc tcgctggtgt atgggattgt
     6181 caccaatccg ggcgaggatt gttcgcacga aatgaacatc agctggcatg cgcagcttgg
     6241 cagcacgaat tgctacgtca cctacacgaa aaagacggat acggcatggg ccaacgcgac
     6301 gacggtgtat ggcaccaacg cctattgcga ccggtttgac ggcatttact ccaagaccgc
     6361 ctcgggagcg gacttttacg agagcgccat cttcctgaat tacggggcgg tgttgagcgg
     6421 gctgacgccg gatacggaat acatgtatca ggtctgcgcc agcccgggcg cgtgcagcac
     6481 gacgcactac ttcaagacgg cgggcgccag cgagttcagc tttatctgga tcagcgattt
     6541 ccacgcctat cccccgctct ccgggcggtt gagcaacgcg atccgggcga tcaatgcggc
     6601 ggtggtgatt gaccccagcg tggatttcat cttctcgacc ggcgacgttg tcgcctgggg
     6661 gggcagctac tccttctgga cgaacctgta tgcgcagaac tttatccggg actacatgtt
     6721 cgccaacgtg gtgggcaacc acgactacat gacccgttct tacgccctca gttcggactt
     6781 tttccgcatc gccaacaatt tcccgctgaa cggctacgcc gggcagtggg gcgtttgtta
     6841 ttggttcatc taccagaacg tgctgtttct gacgcttaac aacgaggtga tgaacggcaa
     6901 taccgccgcg caaacggcgg ccaaaaactg ggcggcgagc gtcattcaaa gccagcgggg
     6961 gcgctaccaa tacatcttcc tggccgagca ttaccagtgg ttctacggcc agagcgggca
     7021 gacaagctgg tatgccaact ggaaggagtt ttgcgaccaa tacggcgttg acctggcgct
     7081 gtcgggcaat aaccacattt atgtgagaac ccacccgctc tacaacgatg cggtagttcc
     7141 caacggacag ggcacggtct atatggaggc gccatcctcg gacggcgaac gcggggtgga
     7201 agcgggcacg ataacctata atgcggaaaa gatcgcttac acctactcca gccatactcc
     7261 ttccggcggc gggcaggtca aaaccatcgg ctgcgtgctg gtcaaagtca atgcccaggg
     7321 aatcacgacc aaactggttt acctggacga taacaaggtg gcgcatgtgg cagacgaaaa
     7381 caccattacc gccgcgcccg tgctgacgca ggcgggttta agccgggtgg tggacgaggg
     7441 ggaagcggtg agtttgagcg tcagcgccag cgggaccgag ccgctgagct accgctggcg
     7501 gtttaacggg gcgccgctgg cgggggcgac cacgagtcag ttgacgtttc ccgccgcgca
     7561 actgaccaac gcgggcaatt acctgtgcgt ggtcagcaat acctccggca gcgtgaccag
     7621 ccgggtgatg tcgctgacgg tgtatccggc gcagcggacg gtgtttctgg atgactttga
     7681 gaccaacagc gccgcgcgct gggtggtcaa ccggagttcg gccgacaccc gggtgacgtt
     7741 tcactacgat tacggggcga tggggattcc ggccgcgccg catacggcgg ggggcacgac
     7801 gcgggggttg cggatggagg cgaacctgac ggccggggtg gcggcggcgg tgtcgttgtc
     7861 gccgctggga cagcagtacc gcggggatta ccgggtgcgg tttgatttgt ggctcaacgc
     7921 caacgggccg tttccggagg ggggcgcggg ctcgacgcag cacgcgacgg cgggggtggg
     7981 gacggcgggc gaccgggtgg agtggggcgg cagcgggagc ggggccgacg ggtattggtt
     8041 tgcggtggac ggggagggtc aggccagtga cacctccgcc agctcggggg attactgcgc
     8101 gtatgcgggg gcggcgctgc agagcgcggg cagcggggtt tatacggcgg gcaccgaggc
     8161 caatgccaag gggaacctgc atgagtatta cgtggcggcg tttccgggcg gggcgaccgc
     8221 gccgggggcg cagcaggcgg cgtatgcgca gcagacggga agcttggcgg ggggcacggt
     8281 ggggtttgcg tggcgggagg tgatggtggc gcggcggggc aacgaggtgg actgggcgat
     8341 tgacggggtg cgcctggcgc ggctgaccaa cgcggtctgg ggggcgagca atgtttttgt
     8401 ggggtattgg gacatgtttg cgtcgctgac ggataatacg aatgtgagct ttggggtggt
     8461 ggacaacgtg cgggtggagg tgccggtgac ggagccggtg atcacggagc agccgcagtc
     8521 gcgggcactg attgcggggg agaacctggt gttgcgggtg ggggcgatgg ggccggggac
     8581 gttgagctat caatggcagc ggcacggcac gaacctggcg ggcgccacgg cggcggtgtt
     8641 gattttgccg gaggtgcagc cgcaccaggc ggggccctac acggtggtgg tgagcaatgc
     8701 ttacgggtcg gtgctcagcc aggaggcgca ggtgacggtg agcccggttt ttgcggtgga
     8761 cggctggcag tcgttatggg gcctggcgcc ggggtcgcgg ccgtatctga cgaccaacag
     8821 cctgcccttc gagcggggga tggcttatga cccgcaggcg cggcgcctgg tgttggtgag
     8881 ccgcgtggag ccgcgggtgt atgtgctgga cgcggagacg ggcgcggatg tgggggagtt
     8941 gggggttagc gggataagcg gcggcattta cccgttgctg atggtggggg tggcggatga
     9001 tggggtggtg tatgcggcca acctgacgac gggcggggcg acgaccgcct ttaagcttta
     9061 tcgctgggcc agcagcgcgc cggggacgga gccgacgctg gcgtatgcgg gcgatcccgg
     9121 ggcgggcaac aatcagcgct ggggggacac gctggacgtg cggggggcgg gggccggcac
     9181 gcaagtgatt ctggcctcgc ggaacggcag cgtggtggcg gtgctgacca cggcggacgg
     9241 gcagaccttc agcgcgaagc tggtgacggt ggcgggggtg ccgaatggcg cgtttggatt
     9301 ggggctgggg tttggggcgg gcaacacgtt ttggggcaag gcgaccgggc agaatctgcg
     9361 gcaggtatcg tttgatttgg cgagcggcca ggggacggtt ctgcgcgatt acgcggcgcc
     9421 gttgattccc gccagcctgg cgccgctggg ggtgagcccg gcgttgcatt tgctggcggg
     9481 gatcaaggtg gggagcagcg gcaatcagct ccagctttat gatctgacgc cgagcaatgg
     9541 cgttccggtg ttggtggggg tgaccaactt tatgacggat tacgacaaca acggcagcgg
     9601 cacgggggcg gtggattttg ggggggatcg ggtttatgcg ttatgcggga ataacgggct
     9661 ggtggcgctg caggttcagg cggcggcggt tccgtggccg ccggcggagc cgggacgttt
     9721 tgaagcgatc caccggctgc cggacggagc ggtgcagttg accatgagcg gcacggcgca
     9781 caccaactac ctgctccaat ggacggccga ctggctaacc tggtccaacc tcggcatgct
     9841 gtcctcccct aatggcttgt tcgcgtgggt tgacggctca gcttccaacg ccccccggcg
     9901 cttctatcgt ttgcagcttg gcccttgatt ccgtccttcc ggaatccgcg cgcccagggc
     9961 gcgggtaacc aaccgggata gccggcctcc ctgtttacag gcttgccggg ggcgcgtatg
    10021 tttgcggcct tgtggcggcc gtaaagaaag agcgcgtcgt gatcggattg agcgggggag
    10081 tagattcctc cgcggcggcg gctttgctgg tggaagcggg ttacgacgtg gtggggatca
    10141 ccctcaagct ctgggcccac gggcgctggc tgccggggac gggcgttgac cgcagggagc
    10201 aggcggttgc ggacgcgcgg gcggtttgcc agcggctggg aatcccccat tacacgctgg
    10261 acgaatccgc cgagttcgag cgctgcgtcg ttcaatactt tgccgacgag tataaagccg
    10321 gccgcacgcc caatccctgc gcgcggtgca acctgcatct caagtttggg cggctccttc
    10381 agcaggcgga aaaactgggg gcgcagtggg tggccacggg ccattatgcg cgccgggagc
    10441 ggagcccgga tggcgcccgc ctgctgctca agcgggggcg cgacctgcgc cgggatcaga
    10501 gctacttcct gtttgcgctg cgccaggatc aactcgcccg cgccttgttt ccgcttggcg
    10561 aaaagaccaa aaccgcggcc cgggcccagg cccgggaccg ggagctccag acagccgacc
    10621 gggaggatag catggaaatc tgtttcgtgc ccgaggccga ttaccggacg tttctgcagc
    10681 agggcgggtt cgcgccgaag cgccgcggcg agattgtgga tctccagggg cgcgtgctgg
    10741 gcgagcatga cggcgtcgcg ttctacacca tcggccagcg ccgggggctg gggatcagct
    10801 cgacgcgccc gctctacgtt attgaactgg atgccgcccg gaatcgggtg attgtgggcg
    10861 ataattccgc tttgatgggg agccagtttg tcgccgaacg gtgcaactgg atcgccttcg
    10921 agcggccacc ggcccggctc gaggcaaccg tcaaactccg ctataaccat gccggcgccc
    10981 cggcgaccct taccccgcgc ggggacggca gcgccgaagt ccggctgcat gtcccccagc
    11041 gcgccatcac gcccgggcag gcggcggtgt tctaccacga tgatttagtg ctcggcggcg
    11101 gctggatcac ccgcagctga agatgccgcc ggaggccggc gacggcggat ttcggggctg
    11161 gactcgattc cgcgatcccg ggtttccccg ccgcccgcgt ttcgccgcat cgctgattcc
    11221 ggagcgccgg cgcggcgaaa gcgggccgga aagaaaaagg cgggggagtt tcgggccggc
    11281 aaacggcggg cggggcgcga gatgaacggg cgcatgaaga tttccttgcg tgacgcccgg
    11341 gaacagggag gcacgcctct ccgctttggg gcgcggccag aaacgggggg atgccctgga
    11401 ttccgcgttc gcgccgattt tgcttcaaaa gcccccgggg gaacaggcat cctgacgcga
    11461 atagaataga ttgaacaaac atgatgcacg agttcaccgc gctggatggc gccattttgg
    11521 tagataaacc cgttgggctg acctcgcacg acgtggtgga tgcaatccgc cgccatttcc
    11581 ggatcaagaa agtcggccat tgcggcacgc ttgaccccaa tgcgaccggg ctgctgatta
    11641 ttgtcctggg gcgcggcacc aagctctccg agcgcttgat gtcggacgac aaggcttatg
    11701 aaggcgtcat caagctcggc gagacgacca gcagccatga tgcggacggc gaactgaccg
    11761 cctccctgcc cgtgccgccg attacgctgg aacagcttaa cgccacggcg gcggagtttg
    11821 tgggcgattt aatgcagacg ccgccgatgg tgtcggcggt caagaaggat ggggtgccgc
    11881 tctacaagct ggcgcggaaa ggaattgaag tgccgcgcga gccgcgcctg gtgcatattt
    11941 acaccttccg gttcagcgcc taccaggagc cgctggcgca atttcgcctg gtctgcacca
    12001 aaggcactta cgttcgcagc ctcgcgcacg acctgggcca aaagctgggc tgcggcgggc
    12061 acctggcctc gttgcggcgg gtggcctccg gcaagttcga ggtcagcggg gccatcccgc
    12121 tggcggaggt gttaaagctg acgccgcgcg agctggagaa acgcgtgctg ccgttcctga
    12181 aattggccgg ggcggcggcg agctaacggg acgggcgcgg gccttggccg attttaccac
    12241 gatgcgcatc attcacgctg cgagcgagct caaccccgcc ggccgcaaag tctgcctggg
    12301 catcggtttt tttgacggcg tgcatctggg gcaccagcag atcattcgcc aaaccctcac
    12361 ggatgcccgg cagcatgacg cggtcgggct ggtggtcacg ttcgatccgc atccgagcgt
    12421 catcgtggct cccgatcggg tgccgccgct gatctattcg ccgtcccgga aactgcgcgc
    12481 gattgaggcc ctgggcgcgg acacgctgct gctcatccgc ttcgaccggg ctttcagcga
    12541 acagacgggc gaggcctttg tgcgaaaact ggcgcgcgac ctgggccgcc tccagagtct
    12601 ctgcgtcggg gccaacttcg ttttcggcca tcagcggcgc gggaatgtgg agttgctgcg
    12661 caagctgggg ggggagctgg gcttcacggt gcatgggatg gcggcggtgg cgctcggcgg
    12721 gcggacggtg agcagcacgc gcatccggga agcgattcgg agcggcgatc tggaccaggc
    12781 cggccagatg ctggggcgcg cctattcgct gacgggcccg gtggttcggg gggatgggct
    12841 ggggcggcag ctcgggtttc cgaccgccaa tctggagacg gcgggcctgg ccctgccgcc
    12901 gcatggggtg tacgcggcct gggcgcaaac cggcaacctg acccggcgcg cggtgctcag
    12961 cattgggctg cgcccgacgc tgcccaatcc gcggccgcag ttgcgggtgg aggcgcacct
    13021 gctggatttc tccggcgacc tttacggccg ggaactggag gtcgtgttcg tggaacggtt
    13081 gcgcgcggaa gagaagtttg cctcgctcgc cgacttgcag cggcaaatcg cgcgcgacat
    13141 tctccaggcg caaacccggt tttagccggc cggggccggc ggggggaagt cggattcccc
    13201 gttggaccgg ccgttgcgcc agagcttgaa ttccttcaaa atggagccgc caatgaactc
    13261 gcgcaggagg gaattgtccg ggatcagctc cgggccgagg gggaggaacc actggaggaa
    13321 cgagagcagg cgcttggcct cgatgtattc cggcgtgcca aagaccacct ggcgcagcag
    13381 cggctcggcc agcatccgca gggcggaaag ctcatatacc agcgcggagt tgaacatcac
    13441 gtccgcattt tcctgatacg ggaagatgtg ctttttctcg ccgcgcccga ccgattccca
    13501 gtggctgatg gtctgctggg cactgtagcc gcggtcgcgg gcgtcgcgga taatgcggcg
    13561 cagcaggcgc gtatcggtgg tggagatgcg gttgtggcgg tccaggttca actgggtgag
    13621 gcaggagaca tagatgcggg tggtctggcc gggagccagc ccgggcagca aatgcgggtt
    13681 gaggccgtgg atgccttcca gaatcaccaa ctggcctttc tcgagcttga cgacatcgcc
    13741 gggctggctg cgccccagcg ggaagttgaa cctgggcaac tgcacttcct gcccggccac
    13801 cagttgcttg aggtcgctca agagccgggt gatgtccagc gcgcccaggg cttcaaactc
    13861 gtaggcgcct ttttcgtccc ggggcgtctt ctcgcgatcc aggaaatagt tgtccagctc
    13921 gagcgggaag ggggaaatgc cctgggccag cagttggacg gcgaggcgct tggaaaaagt
    13981 ggtcttgccg gaggatgagg ggccggccac cagaacaatg cgggcttcgt gggcgcgcgc
    14041 catgacgagc cgggcgatct ccgcgatgcg atgctcgtgg agcgcctcgg aaaccaggat
    14101 cagttcgctc gcccgtttgc tctcgatggc ggcgttgagc gcgccgacgt tttcgatgcc
    14161 cagccgcacc agccagtcgt tgtattcgag aaaggtggcg aggagctttt ttgaatccgg
    14221 caggggccgc agttcattgg gcgcgtggcg gcggggatag cgcagcgcga agccctcgcc
    14281 caccggggtc agcgccagcc agcgcaggta gcctgtcgag ggcaccatga acccgtgatg
    14341 gtaatcgcac cggccgccca gtttgtagag caccagcgta tccttccgcc ggaaccggag
    14401 caactgcacc ttttcgtcct gtcccttgga ttggaagtaa tccatggcct cgcgcaaggg
    14461 caccacctgg cgctcgaaag gaaggtcggc tttgaccagt tctttcatgc gcgcctcgac
    14521 cgcggccaat tcctcccggg aaagcggcgg gcggttgatg accttgcagt agaacccccc
    14581 ggacgacagg gagtggtcaa tcgaaatgtc cgccgccgga aaaacatcgg caaaggcggc
    14641 ttcgagcagg aaagtgatcg aacgccgata aatccccgaa ccgtccgggc tggacatgcg
    14701 gaccaggctg acccgggaat ccagctcgat gggaaaggtc aactcgcgca attcgccgtt
    14761 gacgaccgcg cccacgatgg ggtcattgga ggaccattcc ggcaggcgct tgagaaaagc
    14821 ggagatgggt tggttgcgtg ggccgcaaag aatccggcca tccggcagga ggacttcgac
    14881 ggtggggcgc aaagcagtgc tcagggagat gtcaggtgac gacttcatgg aaaaccttgg
    14941 acgacgcgca aacgcaggga aatgacagga ttacatcgcc cgactggcgg gcctcacagg
    15001 cccgccggcg aaagcgacgg catcagaatt gccgcatcta cgggtcataa gcataaaaaa
    15061 ccaagcatac cttttgtagc tcactccgga ataaagagca agccgcaagc caattgggca
    15121 gaaaatgtca tgccgtccag acggagcatc aagctcccgt ccgccggggt tgccgggaaa
    15181 atgccgagag ccttctccgg ggggaagctc ggtaagttct tcgcccgtag agaggcaagc
    15241 gcaccatacg ggccctatcc agaaaccgta tgggttttga ttgggttcga gctgctcatg
    15301 agatggcggg acaacggtag ggcaatggct tggagatggc cggaagcggg cggggtggcc
    15361 ggcctcccaa gcccataggc tgctggttgc catccaccga atagagacta acgaggctcg
    15421 actatggcag gtcggccatg cctgagcaga ttcttcatgg ccagggcggg caaaccaagc
    15481 cgccagtggt caccttgatt ggcggacgtt ggacgcaaat gtaagatttg gaactggcgg
    15541 caaggcgcct cggacgccat gcgggatgcg ccctgggggc gaaacctctt tcattggctg
    15601 tccacgccgt ggaccgccat cggcagggcg tataaactct tgctcgcggt gataaacagc
    15661 gtttttcgcc ccttgccgcc aaagcacacg ttggccgtcc acggctccgg cacttcgatt
    15721 tgctcaatct tcagcccgtc cgggttaaag accgatactc ctttgccggt gaggtagaga
    15781 ttgccccgat ggtctagggt catcccgtcc gatcccaact cgcaaaagag cttctttccg
    15841 gtgagcgcgc catccggttg aatatgatac cgataggttt tgccggcctt aatgtccgtg
    15901 acgtaaagcc atttgccgtc gggcgtgccg ataattccgt tgggctgctg caggtcgtca
    15961 atcacccggg tcagcgtctg gcaatccggg gccagataat agacgtgttc ccccatctct
    16021 ttggggccgc gcttccaata ggaacggtgg tagtaggggt cggtgaaata caagccgcct
    16081 cgcggatgga tccacaggtc attcgggcca ttaaaccgtt ggccctgata ccccttcagg
    16141 atcacggccg gcttgccggc gcgggcgatt cgccataatt cgttttgggc atcggcgcat
    16201 gcccacagcc ggcctttccg gtcgaaggaa agcccattgg cacgcccaca aggctgcatg
    16261 aaagtgctga ggcggccgtc cacgctccac ttcaggatgc ggtcgttggg ctggtctgtg
    16321 aaaaagacat tgcccttgcg gtccgaggcg ggtccttccg tgaaggcgaa ttcaccggcc
    16381 agcttccgca aggttgcgcc cggggcaata attccgcttc cggcggcccc cgcatgcgcc
    16441 gcgcaagcta tgcccagtac aagttggaaa acgtatttca tttaaatcgc gctgcccgga
    16501 acgaaaccca aacccggctc ggagtaaaga ggcaaaactc tgtccaaccc gcaaagccgc
    16561 tcctctgtcc cgtgcggcaa aactcaagcc ccttcgccgt cacccctgtc ctccggcccg
    16621 ccgccaggcc cggcaattcc ctccccggga ggaacttaaa caaggtgtgg atacccgagc
    16681 gggcctggta aaccagcttc ccgtcgggcg gacgcgaaaa tcggccttcc tgcacccgcg
    16741 caaagcgctg tcccggcgga gcttgcccaa ggcaaatttt tttcgacttt ttcttggcaa
    16801 ttcccgcacg aaggtgtaaa ttaagaaaca ggtaaaccta aagcaataaa agtgacttaa
    16861 gacaacatct tttctccggc ttggtaatcg caactaaaat catatacctc accatgaaaa
    16921 taacataccc tgcactcatt cggcccgccg tgcgcggttg ggcgttaacc ctggctgtgg
    16981 ctggtggcct gcttgccttc gccgggacta cccaggcaca acccacccag gtttactatg
    17041 aggactttga ggtggataac actcagaact ggatcgtcaa ctacggttta ggagccaatg
    17101 tcgccgactt ctatttcgac tatagcagcg tgggcatccc cccggcgccc cacacggtcg
    17161 gaggcactac ccgcggcctg aaactccagg ccaatgtgga tccgaatacg caagccggct
    17221 cggtagcggg ttacggtctg agcgtttctc cgataggatt cagtatcacg gaaaacttcg
    17281 agatgcgctt tgatatgtgg ctcaactaca acgccagcgg tctcggctcc acgcaaattg
    17341 gtggtgctgg atttggcact gcgggcacgc aggcgcagcg ggccatgccg attcccgggg
    17401 tggtggacag catttttatc ggggcttcca cggacggcgg aaccgccgcg gattaccggg
    17461 tatattcccc ggttcactac atcggcctgc aggatgcgag cggagtttat gcggccggct
    17521 cgcgcaacaa cacggccact tattatgtca ccaatttccc cggcaacgtt gcgccgccgg
    17581 cagcccaaac gaacttgttt ccctcccaag ccagcttgcg gaccccggat gggacggtgg
    17641 ggatgcaatg gcgcgatgtc gcgcttcgga aagttgccaa tatcatcacc tatcggatcg
    17701 acggagtcct gatcgccacg attgacgcca gcaccaatgg cacgctgggg ggcgcgaaca
    17761 tcctgttcaa tttttatgac atccagggca atgcgtccac cgatccgctg gccacaaacc
    17821 tgttgtttgc gttatttgac aatgtgcgca tcaccaattt ccccagcgtg gtcacggttt
    17881 cggcgaccac tcccgatgcg gcggaaagcg ggcccactcc cgggatcttc accctcacgc
    17941 gcagcgaacc cggcccggag gtgacggttt tctacgaaat gagcggcacc gcgagcaacg
    18001 ggatagacta tgtgaccctg ccaggctcga tcacctttgc cgcgggggca acatcaacca
    18061 atatcaccct gacgccgatt gacgataacc tgtcggaatt tgccgagacg gccaccctga
    18121 ccatcagcga gggccccaac tattacggag cgggcagcgc cacggtgacc atcgcggaca
    18181 atgacacgcc tacgattgat atctcgaccg tgctgggcag catgtatgag cctctgcccc
    18241 acgattacgt gcgcttccgc ctcacgcgcc gcggcgatct ccaggcgccc agtttcaatg
    18301 tcaacctggt ctattcgggc gaggctgcgg ccacccgcca caccggcacg acgccggtca
    18361 ccgtggaccc gggttcgctc tatacggatt tcgacgtcaa cccgatcgac gaccacctgc
    18421 tgcaaggcaa ccagacgatt gtttgcacgg tgaccagcgg aacgggctat gcggtgggaa
    18481 ccacttctcc ctccgctacc gcaacgattg tggatgatga attgccggcg gagaccatcc
    18541 tctttgccga cgacttcaac acggacactt cggcgaactg gatgctcttg tatgcctcga
    18601 cgaacgcggc cgacaatgat tactccgcca tcttcaacta tgattactcc cagcatccgt
    18661 atgggccgat gatcccgccg gctccgcgtt cgggcaccga tacgcacgga ctgttcctgc
    18721 aggtgaacaa gggggatgct acggaagcgg cagccgcgct gaacctgtat ccccggaatc
    18781 aaaacttcac gggcaacttc gccgtgcggt ttgatatgta tctgattgcc ccggacaccg
    18841 cgacgacgga atacgccctg ttcggcatta atcattccgg caaccaaacc aactggttcc
    18901 gcaacagtgc cgctgggttt gcgggcgtag atcccgttgg ctggactttc gacggcctgt
    18961 tctatggcgt cgagacggat gcggcagccc tgggtgacta tgtcttatac agcgccccga
    19021 cgacggcagg ccggaatcct accgcgctca actcgcggtc ggcttcgacg ctcaccggca
    19081 tcttcaaaaa gccgccctat ggctacgccg gttccccggg gaacctgtcg ggctcctcca
    19141 cgccttgctg gtcggaagtc gagatcagcc aggtcggaaa cctggtcacc ctgaaaatca
    19201 accaaaccga gatcttcagc tataccaaca acacggcatt caccagtggc aatatcatga
    19261 tcggacactg cgatgcctac gactccgcgg ccacttacga cggcggcgtg atttacgaca
    19321 acgtgcgtgt aatcagactg ccgacttccc tggcgccggc gacgatcacc agcattcagc
    19381 ctgtgggagg accgccgacg ggctatctgg tcaattacac cggcggttcg ggatcgcaat
    19441 tcgtgctgct aacgtcgccg acggtgacgg cgccgttgtc cagttggacg cgtgcggcca
    19501 cgaattcgac cggcagcggg accttctcgg tcccggcggg cgcgcaggcg ttctatcgga
    19561 ttaagagcga ataagcgctt ctttcgaacc ccgggccggt tttacgccgt cccggcagcg
    19621 accgctcggt ttgcgggcgc ggaggtgttg ctggctggca acgccccgcg tcctgtttgc
    19681 ttatttcgct gggagcactc cccaagcggg aacgggtcaa gctgtagcgc cattcctcgc
    19741 ctgtcggctt taaggtttta atcagcgctc ttgcgcgggc gacaggcgcg ggtcgtctaa
    19801 atcgagccga tagagcacct ggttgtaatc atggcgcggt gtgggagaag gccgaccggc
    19861 aaactcagta gtgtaggtgc cctcgaagat cagaatgggc gaatctgcgg gagtaaattc
    19921 cgggtgcagg cgcggattgt agaaggaata attgtcatga ctgagcacct tgaccgcctt
    19981 gccccacggc ccggtggggg catctgcttc ggcgtaccag agttcgccca tggccgacgg
    20041 tttgccaaac gactccataa agatggtcac ccagcggcgg cgccagggat tccaggcgat
    20101 gtggcccgaa tgcggtttga cggctgcacc atccgtcgcc gaagtgaagg cggattgggg
    20161 ttcgagggcc tgccattgtg aggggtcttg ccaggcctca aaactggcgc ggcagcgcat
    20221 tatgggcagc ggattgccaa agagcaccca cgtctgttgc ccgtgggcat ttgtccagaa
    20281 agccggctgc ccccacggtg ccagggtcgg cttgggttgg gaatcggact tggtccacag
    20341 cacccgatgc ggttcgaaaa gctggcggtc gtcgttccac acgcaaagtc cacattcata
    20401 ggcttccatg cgtggtttaa tcttggtgta agtgctcacg aaccgcgggg tgccgtgttt
    20461 atccggcagg ctgacgcacc cgctaagcca ggtggggccg ctgccaggca accgcgccac
    20521 cgcacgcgga accccattgg tatcagcaaa gtaatccagt ttgagccgga gtggcggcgc
    20581 aaagccggcc agcggctgga tggcactggt ggcgcaggtg gtatcaaaga tgcccagtgg
    20641 atactgcggc acgatagtat ccccccagat ccaaaagagc ttcccgcgat ggacagcgtt
    20701 ctggacgcta tcacaaccca ggatgcccga ttcgcgccaa gtaagttcgc gcccgcattt
    20761 ctggctttcg gcaaacaggc cgccaccggt gatgcgcccg agtcggcggg cgatgatggt
    20821 gcggttaact tcgattttaa gcgtctggcc gggctgcggc gccaggcgcg cgccacggta
    20881 gccaaaacca tccttaggtc gctcgtagcc ctgcccatgc acatcaaacc acgtctcgcg
    20941 ccccatcaac tccggcaggt cgaacgcgat gaccccggcg ttatccgtga cgaaccggac
    21001 actgtgcgtg gtgcgcaact caaccatcgg caccggccaa cccgtgccgt gctcaatcac
    21061 ttcgatgcgg caaggttcca ccgcccgaag cgcgaggggc ggccaggcta aaccagccag
    21121 caacagcacc ccgcaagccg agaccgggat tctcatacgt cggcgggcgg ggaggaggtt
    21181 gattcctgcc cttccaatac cgcgactgcc gcggcgtaat tctgggtgtg gctcaagctg
    21241 atctgcaacc gctgcccgcc acgggccgcc agcagttgcc ggcccgcttc gtgcagaatc
    21301 acaaagggct ggccgctagc gtggcggcct atctccatat cgcgccagcc aagctgagcg
    21361 ccaatgccag tgccaaacgc cttggaaatg gcctccttgg cggcaaagcg cgccgccaag
    21421 aacggggcgg gcgcccggtg cgcgaggcag taggcaatct cgttgggatg caatatgcgg
    21481 ttcagaaacc gttcgccgaa tttctcatgc gaagcctgaa ttcggctgac ttcgatgatg
    21541 tcaataccca cgccgagaat catgtcgtct gtctgaggcc gaaagccgga tacggccagg
    21601 agtccagcct taagccctcc tcaacccggg tagccagcca tcaagcgcag catatcctgc
    21661 acggcggtcg tgaggccggt catgacggcg cggctgataa tggaatgtcc gatgttcaac
    21721 tcgaccaaat gcggcacctg gtgaagcatt tgcagattct gatagttcaa gccgtgcccg
    21781 gcattgacgt tcaggcccag cgcgtgagcc tgcctggcgc ccgcgatcag ccgttccagt
    21841 tccgcctgcc gatgcgcctc ctgatcgaag gcttcagcga atgccccggt atgcaattcg
    21901 atgaattgac tgcccgcccg cgcggatgct tcgatttgcg ccggatcggg cgcgatgaac
    21961 agactgacct cgatgccggc atcgttcatg cgcttccggg tctcggtgat caccgccagg
    22021 tttcccgcga cctccagccc gccttcggta gtaatttcct cccgccgttc gggcaccagg
    22081 caaacgatgt ccggcttgag gcgaagggcg atcgcaacaa tctccggcgc aacggccatt
    22141 tccaggttga gccgcgtctt gatggcctct cgcatcttcc aaacatcgcg atcctgcatg
    22201 tggcgccggt cttcgcggag gtggacggtg atgccgtgcg cacctgccgc ctcgcaagcc
    22261 agcgctgcgg ccaccgggtc gggttcaccc cgctcgcgcc cgcggtagcg cgcttcgcgc
    22321 aacgtcgcca catgatccac attaacgccc agtttcaaca taccggtgat tatggtttag
    22381 cccgcagcca gttcgcaacc taaacatatt tgccattgcc tccttcggca accttctgtt
    22441 tccatacgcc gtgccaagaa agcaattatt catagcgcgc tggctgctgg tgttgtgctg
    22501 gatgggggta atcttttccg cctccggcga ccaaatgtca ttcgaccgct cccggcacat
    22561 catcggctca tttctgtgct ggctggtacc gcagctttcg cccgaagcaa tccatgctgt
    22621 ggtggtcgtg ctgcgcaaat gcgcccacgt ggcggagtac gccgtgctgg cctggctgat
    22681 ctggtgggcc ttagaccagt tctcggtttc gcgcgcccca gactggccct ggcctaaagc
    22741 cggcctggtc ctcaccctgg taactctcta cgccataagt gatgaactcc accagacttt
    22801 cattccttcg cgtggaggtt gtgtgcagga tgttttgctc gataccctgg gcggcgcgct
    22861 cgggctgctc gttttgtggg ccgcctgccg gttgaaaaaa cgcgaataag ccgcctcctc
    22921 tttatccctt ccaccccgca gccccggcga ttcttatcgc acgaccttca gccagcccag
    22981 cgatgccggc acatgaaagt ccgggtcccg ggttttcgga tccacccagc ttatccattc
    23041 ctcgacgggg ggcggtccct ccgctttccg gcccaggtta tgcacgctgg tttgcggctc
    23101 gattggccgc ccactgcggt catggctgaa ttccgcccgg tagaggccgc cgcgtatctt
    23161 cactccggga cgcaaggccg ggaaccccag tgccaccagg ctttccaggg gaatacgccc
    23221 ctccacctca tatccgccgg gcaacagcac ccctcgggtt tccagcccca tgcactgcca
    23281 tttcgcttcc aaacgccgat aatacgagcc gcgatagtca tacacccgtc cccgggcatc
    23341 cacttcgaag cagtaataat cctgcatctg gtcatcccgc cctaaaaaca cctcgacgcg
    23401 gtcttctaac accacatcca actcgtcccg caaatgatcg agcaccacgg tgtccgcatc
    23461 ctgcacacgg aacgtaaaaa agagatgcat cccgtcgcac aacgcacgga attccgtctt
    23521 tggagccttc gcccgtttcc agggaaacgc aaaccgccgg ttcactaccg cttgcatcca
    23581 cgccggttca tctgcccgcc cgtccagcgt gatctgcgcc ccaggtcgcc accgcactcg
    23641 gtaggtctgt cgggatggct ttccgggtgg agggctcagg ggactgcctg ccaccgccga
    23701 ttcctccgca ccggctcccg ccacgaccag caaaaatgct atcccaaccc gtttagctgc
    23761 caacgccagt tgcttcttca tgcgattcag cctgcggcca ttccgctgcc aacgcaagtc
    23821 tggccatgat tctcgtaata ggaaatcagg tctcaccaaa atgtcttccc ctactgatcc
    23881 agccggacaa ctgttaacct tttatggccg ccaaggcttg acaaatggcc ctttttaccc
    23941 catagatatg gtgtccgagt gatagaacgt ttcttcagcg gggttcccca cccccacctc
    24001 cttggcgtct atcgctcgga cattcaacga acgcccgtgc tggttttcgg ccttccaatg
    24061 ggcggctttc gggccgttgg gagcatgaat ctcgccgcca actctcaagg gtaaagaaat
    24121 aaacgattgg caaaggcacg ctcgatatgc tataaacacg ctccgcttct gttgggagcg
    24181 gttggaaacg ccagtaaaag agaataggaa tttgttttat gtcacagcat cggagtctgc
    24241 gcgcggcagc cactttgggc ggcaagcgga atgtgctcaa gcgttttgag cgaatcgaac
    24301 tgctcaaaaa gcgcggtcaa tggaaggaag gcgaccgcat cactggtttg cgcaagacca
    24361 aaaaagacgc ctaaattggc actttaacgc agcgcacggg atagcgaatg ctaccgggcc
    24421 cctgcctttt ccagatggtt cgcctgcaaa agttcctggc ggatgcgggt attgcttccc
    24481 ggcgcgtagg tgaacagatg attctggcgg gtcgggtggc ggtgaacggc caaaccgccg
    24541 gtgaactggg attccgaatt gacccggccc gcgaccgagt ggcggtggac ggcaagccga
    24601 ttcgggcgaa gcgcaaggtc tatgtggcat tgaacaaacc ccgaggggtg gtctgctccc
    24661 ggcgtgatga atttgagcgc ccgacggttt acagtttgtt gcccggggaa tggagccatc
    24721 tgcatacggt tgggcggctg gattaccaca gcgaaggatt gatctttcta accaatgacg
    24781 gcgagttcag cctgcggctg acgcatcccc gctacggggt gcgaaagcgc tacgtggcaa
    24841 ccgtggaagg ccgggtggcg gaggaagtct tgggccagtt cacgctgggg atatggcacc
    24901 agggcgagaa gttaaaagcg gagcgaaccc ggctggtttc cagtggttcg acgcaaagcg
    24961 tggtggaatt ggaattagct cagggcaaaa accgggaagt gcggcggttg ttcgaatcgc
    25021 aaggtatgac ggtcaaacgg ttgcagcgga tccagatagg gaaaatcaag ctgggcgagc
    25081 tgcgaccggg taaatggcgg atattgacag agcccgagat taaatctcta atgtgctgaa
    25141 ttatgaaata caactgttgg ttggtgttgg ggataatgct ttcaacaagt ttgctagcgc
    25201 aagaggcaac ggatacggcc ccgcccgcgg ccatccaaat gcccgtggcg gcaccgcctg
    25261 cggatctggc gccggccgcc gccaaaacaa acgcgcccgc agccaaggcg gcgaaaaaga
    25321 aatccacccc aaagatggcg cacaaggcgc ccgtgaaaaa ggctcccgcc cggaaaacgg
    25381 ccacctggcc ggaattgcga acgatgccgc tggtcgccgg cccggcaatg gtcgtggcca
    25441 gcaacgtcaa tgtgcgtggc caggccaaac tcaagagcga gatcgtgacg caagtgacca
    25501 aaggccagac cgttacggtg atcgaggaga ttgtgcgcaa taattccggg ccggacgaac
    25561 cctccgcctg ggccaaaatc accttgccgc ccggcgctcg cgcatgggtg cataatgcct
    25621 atctcaaggc cgctgaccaa acggtcgtgc cgcggcgact caacctgcgc agtggccccg
    25681 gcgagaatta tagcgtgatt ggcctgctgc accgaggcga cgtggtgaag ccaatcatca
    25741 cgaaagaaga ttggaccgag atcgagccac ccgcaatcgc ctatgcgttt gtggcggcac
    25801 aatatctcag ccaggaagcg ctgccgccat cggttcccac accgaccgcc gtgactgcag
    25861 ttccccccgt cgccccacca ccggaaccgc cgcccgcgcc ggccacggta acggtaatcc
    25921 cgccgggacc ttcgcaaccg gttgaaccgc cgcccacgcc gaccacggtg gcggcagatc
    25981 ccacggtggc tccccccccc gctgaacctc ccgccctgcc tcccccaacc gcgactcccg
    26041 acgtaacgac gctggcggaa actcccgctg cgccaagcga accggaaatg atcgaaaccc
    26101 cggaagaaga ggaggaaccg ctgccaccgc gcatcgtgga gcgcgaaggc atcgtgcgcg
    26161 gcatgaccag cattcaagct ccctccccat ttgtgttggt gagcccggaa accggtcgaa
    26221 atattgatta tctctacacc acatcccggc aactggatct gcggcgttat aaaggcttgc
    26281 ggatcatcgt gaccggagag gaggctctgg acgagcgatg gggcactaca cccgtcatta
    26341 cgatccagaa aatccaggtg gtggagtaat ggctccagcg aaccttattg acggacgggc
    26401 cattgcccgg caactgcaag gcgagctggt ccagcgcatc gaggcgctca aagccagtgg
    26461 cgttcagccg gggttggcct tcgtgcgtgt gggcgaggac gccgcttcga aagtttatgt
    26521 cggccgcaag gaaaagatgt gcgccgagct gggaatcgct tcggaaacac acgtcctgcc
    26581 ggaacaaacg agcgagcctg aactccaggt gctgctggat cggttgaatg ccgaccggca
    26641 gctgcacggc atcctggtgc aggcccccct gccgccgcac ctccgcgcga cgacggttta
    26701 cgcccgggta agcccggaaa aggacgtgga cggttttcat ccgctcaatg tgggcaaact
    26761 gatgctgggc gatccgacgg gatttctccc ctgcacccct gcgggaatac gcgaattact
    26821 cgcgcggtcg ggagtccagg tagcccgggc ggaggtggtg gtgctggggc ggggcaacat
    26881 tgtgggcaaa ccgatggcgg ccatgctctg ccagaaagct ccccacgcca acgccacggt
    26941 gaccctatgc cattccgcga cccgggacat cggggcgcac tgccggcggg ctgacattct
    27001 catcgccgca atgggagtag ccgaatttgt gaaggcagac atggtcaagc ccggggcggt
    27061 cgtggtggat gtgggcgtca atcgcgtgag cgatccgtcc gccaaaggcg gctcccggct
    27121 ggtgggggat gtggatttcg ccgccgtgca accgatagcc ggccaaatta cccccaatcc
    27181 cggcggcgtg ggccccatga ccattgcgat gctgatgcag aacaccgtcc gggcggcgga
    27241 acacgctgcc cggtagaaag gcattcatta tgcaaccgac ccgaatcctg cccgacctgc
    27301 agtgctcgct gatgtgcgaa gaagttcgcc aggaggccaa cgccaacttt ttcctgatcg
    27361 gggtgctcgg cctcattcgc gtgccacaat tgccggtgat ggccttcaag ctgagcgtgt
    27421 tcaaccgttg gaccgcggga gtggggcagt ttatcgaaac cacccgcttc atcgccccgg
    27481 atcaaaccac cgtgctgcgc aaaggcgagg tcaagttcag tttgaaggaa gccggccagc
    27541 acgcaaccaa cgtgacggtc ttcacgcagg tggagtttaa aaccgtcggc acctactatg
    27601 tggaagtgct ggtggatgac gtcatgaaac tgcgctatcc cgtgccggtg attcacacgc
    27661 cgccacccca gccgccatca gcgccaccgg ttccgcccgc gccggcgagc taagccgcct
    27721 cccccggagg cggaccgccc ggcacggcac gaaagccgga ctgccgccgc aaacgcgcgg
    27781 gacgcacatg aatgccccag atgccaaaac acaggctgcc tggcagccgc tgaccttcaa
    27841 aggcgtggcg gcttttgccg gcgggacgat gagccaatta ttgctcattc agatcgtcgt
    27901 ggctctgctg gccggaagca cggtgatctg gcttgtccac cgggcctggt ttcccacgat
    27961 tgaggaagcc atcaactccc tgccgcccca gggagaacta cgttccggcc aattgctctg
    28021 gacgggagat gcgtcggcac gcctcgccga agggcggttc ctggcgctca ccgttgacct
    28081 ggatcatgca ggcaaggtgc gctccccggc gcatattcaa atcgaattcg gacggaccga
    28141 cttccgggtc tattccttgc tcggctcctg ccagggaagc tatccgcgcg gacaatccct
    28201 ggccttcaat caaacggaac tccgcccctg gtggggagcc tgggccccgg caatcttgat
    28261 gataacgggg ctgggggtgg cggccggact gctggtgatc tgggcggggc tggcgaccct
    28321 atacactttg ccagtatggc tggcgggtat tttcgcggac cggcaatgca gcctgggcgg
    28381 aagctggcgc ctggccggcg cggcgctcat gcccggcgcc cttttgacct gcgcggcgat
    28441 tctgctgtac ggcggagggc gcctggatct attgcaattg gccgcgatcg gcgtggcgca
    28501 tgtaatgctg ggctgggttt acctgatcct cagccttttg cgcctgccgc ggctggagga
    28561 agccgaggcc gtccggagga atccgttcgc gtgattgcct gtcgccgccc attcagctaa
    28621 cttgtggcat gatgttttct gtgggcgcat atcggtcggc gttgttggcg ctggcctgct
    28681 tgctcttgag cggctgctta ccctccgccc cgggggagga ggaaaaggaa ccgcattttc
    28741 tggcgggccg aaatcgcgtc aacagcctgg acttcagggg ggccattgaa tccttcgaaa
    28801 aggccttgga agcaaacccg cggtcggcgt cagcccactt cgagctgggc tggctgtttg
    28861 atcaaaagga acctgatccg gcggcggcaa tctaccacta cgaacgctac ctcaaactat
    28921 tccccaactc cggcaaggag gaattggtga aaacccgcat cctggcctgc aagcagcaac
    28981 tggcccaggc gatttccctg gcgccgggga tggaaaagct gcagcgcgac ctggaacagc
    29041 ttacaaccga aaacaaacgc ttgcgggaag aactcgaaaa atggcgcgcc agcgcctcgc
    29101 gcctgcaaag ttctgccgca tccccgactc cggccatccc cggcccccgg gaggttcaac
    29161 tgcccagccc cagcgcggcg gtgacaaccg gcgcggcact gcccacgggg ggaagtccga
    29221 accggctccc gagtgtgccc ccggctcccg cgcgaaccca taccgtaaaa aaaggggaaa
    29281 accccactac gatcgcccgg atttacggcg taaaggttga gtcgttgttg gccgccaatc
    29341 cccggctgaa tccacggcgc atgcaggtag ggcaagtgct gaaagttccg ccgcgctagg
    29401 cgggcagttc tccgggcgtc gcgggtgcgc ggcggacgcc gcggcaagcg ccccggatgc
    29461 cgcgaaagcc tgcgcctctt tgctatgcgg gttcgacgcc ctggcagcgc ccgagcgggg
    29521 ctaattccct cgcggcttgg gtttcagacc gcgcaaatgc tcgtcaaacc aatcggcgaa
    29581 gagttctata tccctgctcg aatccggcca gccgtgctgc ttgccttccc gcacctccaa
    29641 ccgggcaatc accccctcct cccggcagcg ttggacaaat gtctggctct ggtaaatggg
    29701 cacgagcttg tcggcatcgc cgtggataat aagaacgggc ggcatgttgg agtggacaaa
    29761 atagatgggc gaaatctccc gccccagctt ctgccgttcc tcggccgtgt ccgcgcgcgg
    29821 gccgaatgca gccttgaaat cctttaacac ccccactccc acagcgtctt cgcccggccg
    29881 gctgtagttg agaaaatcag tcggcggaaa gaagcaggcg accccctgca ccgcgctgct
    29941 ttcacgatcc acgggatccc tggcctgcgg atcgccggga ccgccttgcg tggccatggt
    30001 gagcgaaaga tgcccgcccg cactgccgcc agtgatgccg aggcgattgg gatccacccc
    30061 ataccgggcg gcattatgcc ggatgaagcg caccgcacgg tggatgtcgg gcacgatctc
    30121 cgtgatcacg aacttcggct gggaaccatg caccacggca aaaacggtat agccgcgctt
    30181 cagcaggggc ttgaggtaat tcgcgttgat aaactcatgc gccgagaccc agccgccgct
    30241 gaccatgtga agaatcccca ccccattggc cggctgcggt tgaataacat ccagcgtgag
    30301 ggcggtgcca aatttgcgcc cgtagatgac atcttcggta cgggtgaagt tatcattcgt
    30361 ttgagcgccg acactggccc cgagcagcag caggcaggcg cccagcacat ttttcatggc
    30421 atgcataggg gcaatctaac ggcagccttt gccgggagca agcatcccca ctacgcgccc
    30481 gcgcccgcgc gcaaagccct gcggcgccaa cgggaaagcc tcacacgtta aacttgaaga
    30541 gcagcacatc cccgtcacgg acaacgtagt cccggccttc gagacgataa tggccggttt
    30601 cgcgggcgcg gctgaccgaa ccttctttga ccaggtcagc ccaattcacc gtttcggctt
    30661 tgataaaacc gcgctcaaaa tcagaatgga cggtcccggc ggctttgata gccgtggcgc
    30721 cggcgggaat cgtccacgcg cggacttcct tctggttgaa agtaaaaaag gtgcgcaacc
    30781 ccagcaaatg gtaggtgctt tggatcagcg cggcgccgcc cggctcccgc acccccagct
    30841 cccgcaggta atcctgcgct tcttccggcg aaagatcggc aacctcgctc tccaactgcg
    30901 cgcagaccac cacggtatcg caggcctggt gcgcgcgggc gtactggcga actttggcaa
    30961 cctgtggatg cgtatccacg gcggccaggt cgctttccgt cacattggca acgtagatcg
    31021 tgggcttgtc ggtgaggaga aagaagccgt ggacgatgat tttctcgctg ggggccagcg
    31081 gcaggccgag cgtattggcg ggctggccag tgttcaggtg gtccccgagt ttctccagca
    31141 agtgcgcctc caccagggcg tgcttgtcac cgcgtttaac ctcgcgcgca atcttttcca
    31201 gccgcttccg gaccgtctcc aggtcggcca gcaccagttc ggcggtcaca atctcgatat
    31261 cccgcaccgg gtcaatgtcc ccggcgacgt gaacgatatc cggatcctcg aaacagcgca
    31321 ccacttgaac gagcgcatcc acctcccgga tatggctgag gaacttattg ccaagcccct
    31381 ccccatgcgc ggcgcctttg accaggccgg cgatgtccac aaattcaaag gtcgtcggaa
    31441 tccgcgttcc gaccttaacc acctgggcca gttggtcgag ccggggatcg ggcacgctga
    31501 caacgccgag gttgggttcg atggtacaga acgggtaatt ttccgccggg gccttgtgtg
    31561 accgggtgat ggcgttgaag agcgtggatt tcccgacatt cggaagaccg ataatgcctg
    31621 ccttaagcat gacgctgtta tagctggaag gagcgccggg gacaaaggga aaaacacggc
    31681 cacaatcgcc tgccccgcca gcaactcgac ccccgagccg gcagcctaaa acgcgcccgc
    31741 ggctacccgg cagcggcgcg aatggcctgc ttgaactccg ccaggccttc gccgaaggcg
    31801 gcggccatgg acaggatcga aacccgtcgg atgcggcact tgaacgcttt caggttctcc
    31861 cgcgccgccg cctcgtccat tttgttggcg acgatcaggc gcggcttctc caccagcgcg
    31921 ggatcgtaaa gctccagctc gcggagcagg ctccggtaat cgtcccacgg agcgcggccg
    31981 tcggttcccg ccatgtcgag cagcagcacc agcactttgc atcgctcgat atgccgcagg
    32041 aactcgtgac ccaacccgac attgcggtgc gcgccttcga tcaggcctgg cacatcgcac
    32101 acagtcaggc gatgataatc cgggtattcc acaatgccga tttgcgggtg aagcgtggta
    32161 aacgggtagg gggcgatttt gggccgggcg cgcgagatgg cggtaagcaa ggtggacttg
    32221 ccggcgttgg ggtaacccac caaccccact tccgccatga tgcgcagctc caggagaaag
    32281 ttgccttcgc cgccaggctc gccgggttgc gcaaagcgcg gcgtctgccg cacagccgtg
    32341 gcaaaattgc gattccccaa tcccccgcgg ccgccctggc acaacacaaa gcgctgcccc
    32401 ggttccacca ggtctatgac caactcgccc cgcgactccg gaccggaagc cagtcccccg
    32461 gcggcgggga cgctgacggg aagcgccgcc tcctccccat ccgcgtccgc ttccccgggc
    32521 agagtacgaa gcatccggcg ccggcgcgat tggctctgat attcccggcg agcggaggtt
    32581 cgggagattt cgccgtccgc cgcggcatcg agccgccaga ccagcgtgcc gcagggaact
    32641 ttgaccacca ggtccctgcc cgcgcggccg tccatccctt tgcccaaccc gggttcgccg
    32701 tccgccgcca gcaggcgcgg ctggtagaac tgggcgatca ggttgttcag gtcatgatcg
    32761 gcttcgagaa taacgtcgcc gccgcggccg ccattgccgc cgctcgggcc gcccttggga
    32821 cgaaacttct cgcgctggaa ggcgacacag cctttgccgc cgtggccggc tcgcgcataa
    32881 actttgatct cgtcaatgaa cattagaacc ggcgcaacgc aagtgttcgg cgagcgcgaa
    32941 gcggccgccc ggcaagcccc cggcacttca gcgccacccg gggttcccac cgcgccgcgc
    33001 tccgctttcc ggctgccatc acttcgatcc ccgcgaaaaa aaaggcgagg ccggagcctc
    33061 gccgccaaga gttgtccaac acgcttaact gccggtggga gccgcttccg ccagcacgtt
    33121 gacgcggcgg ctgttcctat cgaacgccac cctgccgtcc accagcgcga acagcgtcca
    33181 gtcgcgccct gtgccgacat tccgcccggc aacaaacttg gtgccgcgct gacgcactag
    33241 aatgctgccg gcgctcaccc gttcgccgcc aaatcgcttt acgcccagcc gtttgctgat
    33301 gccatcacgc ccgttgcgag cgctaccctg accttttttg tgtgccataa attcctctct
    33361 gctgcttgtt aacgagtatt tgctgacgcc ggtttagagc ttgatctcct taacctttac
    33421 aaccgtcagc tcctgccgat gcccgatcgt cttgcgatag cccttgcggc gcttcatctt
    33481 gtaaacgagc gtcttgtcgc ctcggatgtg cttgaccacc tcggccgtca ccgtagcgcc
    33541 ggtaacggtc ggggcgccca cggtgacctt gccgtcatta ttcaccagca gcacgcggtc
    33601 aaagttcacc gcctgcccgg cgtccaccgc caggcgatca acttccagcg tatcgcccgc
    33661 actgacccga tactgtttgc ttcctgtttc taatacagcg tacataaatt gttcggacta
    33721 aaggaggcgt gatgaaacca gaaccccgca gcggtgtcaa cacgtcagtt tgcattatga
    33781 atagtccgtc catgccgcaa aagcaagcct caagacggct ctgaccaggc tgccgccaga
    33841 ttccgccccg gctggccctg gacatgcccg cgcaattagc cggtcgggct ggccgcagcc
    33901 tgcaatctgt cttcgagtag ccagacaggg ccaggcttaa gcaactcgca acgcttccct
    33961 ggaaccatgc cccagcaagg acgaaaaccg gccattggga ggacttgccg cagattacgc
    34021 aactattgga cgctttcggc tatttccatc gaggtctgcc cccttctttc cgtcccagca
    34081 tccaagctaa acgccgtttt tcacctaacc tccgtctttc caagcctttc gatagctgcc
    34141 tccaagccgt gtacaagggg tgtacaaggg atgcccaccg ggtgtacatg ctaaaatcct
    34201 atgaacttct ggtgagcatc ccttgtacac cccttgtaca cggctctttg ggacggtgtc
    34261 gaaacgggga tgggaagctg ctggaattgg gggattaaag ggatggtttg cggtctatgg
    34321 gccgcgacgg attggggctg tggcgtatcg gctggccag
//
LOCUS       DAOPQG010000027        34144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1678_length_34144_cov_14.874916, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000027 DAOPQG010000000
VERSION     DAOPQG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 34144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34144
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1678_length_34144_cov_14.874916"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(490..3225)
                     /locus_tag="PKI07_04695"
     CDS             complement(490..3225)
                     /locus_tag="PKI07_04695"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOC50383.1"
                     /translation="MNQSKSGAGVPPSAARQFLWIALCLAVLLAVLFRDGLRPGHTVF
                     SNDGPLGAISSQSARLPAGFFSYWGDLNWLGGTGPSASPDISQGLALLAGPLLFSKIY
                     APFTLLFLGLSAWLCFRQWKFSPRACFLGSLAVALNSDFFSTACWGVAAQPLSFGLDF
                     LALAALADLSSPRRWLRAALAGLAVGLGVMEAFDIGAIFSLVIAGFVLWQAWAGEGTR
                     AQRAARGVGRLAVVAAFAAFVAASAVSTLVGTQIKGVAGMGQDQASKAQRWDAATQWS
                     MPKRETLSLFVPGLFGYRMDTPMNLPEGLQNYYLGGCYWGAGGRHPEWDRYFAAGKPG
                     PPPSGFIRYGGGGVYAGTLVVLVALWTMLQALRREQSVFSRRERKFIGFWSGVTVIAF
                     LVGLGRFAPFYQFFYALPFASTIRNPAKFFHVVDWALVILFACGLHGLSRSFLDAPGA
                     ALRELPAQLRTWWARAGRFEKNWARGSALALAAAGLAWLIYAQSRPSLETYLQEVQFD
                     AGMAREIAGFSIRQAGVFLVLFTLALGLMLAVVSGYFSGRRARLGGILLGVLLVADLG
                     WANMPWVISWNWQQKYATNPVIDLLRDKPYEHRVAVLPFVTPREYALFHQLYEIEWKQ
                     QLFPYYNIQSLDIVMMPRAPVEFLAYESALGSDGTPGTRHRVARRWQLTNTRYLLGAA
                     SYLSALNRELDPAQNRFRIATRFDIETKPGIDRATTLETLTAVIKPDGPYAVFEFTGV
                     LPRAKLYANWQVSTNDQATLTTLGSAEFDPAQQVLLADPLPAPDPLNATNPNPGTVEF
                     ASYAPKQIVLQAKAETPAVLLLNDKYDPNWQVSVDGKAGKLLRCNYLMRGVYLPPGSH
                     TVEFRFAPPTGILYVSLTALAVTVLLLVCLVFPRLKEPAPPPSARKPSKPGKPPG"
     gene            3278..5224
                     /locus_tag="PKI07_04700"
     CDS             3278..5224
                     /locus_tag="PKI07_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50384.1"
                     /translation="MNKPQAAGPGGGVFRSGWFWAASLFLAGLGARLWLIDRFGTALP
                     FWDQWEEARVVYVPFLAGKLSLADLFSAHNEHRILFTRLYGLALLLLNGQWDGRLQMV
                     GNALIHSATLAGLGGLMFRWLERKYWVWVWLPLALVLALPFGWENSLAGFQSQFYFLV
                     LFSLLTLWLLGLHPPGSAPWWCGAATALMALFTVASGFLAAAAVFALVLLRLGRQREG
                     WRREAVTLLFCAGVSLAGLALKTDVRHHQALQAHSLGDFVAALGANLAWPWIVVPPFA
                     VLNLLPLVALGGFYLGARRARHPAEEMTLALGLWTVLQGLATAYARGAEGKPPGWRYM
                     DSSCFILVAGCFSIAILLSRYLAAARARRAGQVIFGLWGIGCAAGLVLLTKRAWQVDI
                     PERQFYFRCQLLNTRAFLATRDLRVLDHKPNPQLALYEGDPYAPRPAHAGAKLAAYLD
                     YPGVRGFLPACVRDPLTLVPLHNHGFATNGAARARPRIPGEVSWGSDTEAGAAATGSF
                     ESAPIPASKRPFLEFRVAGDLGKPGLSLAVVELSSGQARAVQPRPAPGNTWRTCQVRA
                     PRGAFKVVARDASADGWFAFQAPREVGGLSWAAARLAALGGGLFFAGLGLGLLGIVGG
                     GRFRPGADSSRRAAGDGPPSQSAV"
     gene            complement(5304..6074)
                     /locus_tag="PKI07_04705"
     CDS             complement(5304..6074)
                     /locus_tag="PKI07_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC50385.1"
                     /translation="MSTPAPRPPRPLSLLSVVIPARDEEGCLGSTVEHLHLELRLRNI
                     PHEIVVVDDGSTDGTWKLLCDLQARLPELKPLRNEGLHGFGRAVIRGLDHLQGDAVVI
                     MMADESDDCRDVVRYWQELNQGWDCVFGSRFMKGGGVVDYPKIKLLINRMANLFLKML
                     FGIRLNDTTNAFKAYRREVIDGCRPLISPHFNLTVELPLKAIVRGYSWTVIPITWRNR
                     RTGVAKLKLREMGSRYLFICLYVWLEKYFSRGDYRKPR"
     gene            6344..7405
                     /locus_tag="PKI07_04710"
     CDS             6344..7405
                     /locus_tag="PKI07_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC50386.1"
                     /translation="MSKACLVTGSSGLIGSEVAAFFHERGFGVHGVDNNQREAFFGPQ
                     GNTRWNQERLQRTLAGFTHHEVDIRDRAAVLELVKTLQPAVIIHTAAQPSHDLAARIP
                     FDDFDVNATGTLNLLEAARRYCPESPFIHMSTNKVYGDRPNTLALQERETRWDYADPQ
                     FAQGIPESFPIDQSKHSLFGASKVAADVMVQEYGRYFGLPTCCLRGGCLTGPNHAGVE
                     LHGFLSYLVKCNLEGREYRIYGYQGKQVRDNIHSVDVAGFMFEFYQAPRAGEVYNLGG
                     GKDNSCSIREAFAMAEACTGRKQVHTYVEQARSGDHICYYSDLRKMRAHYPRWQLTRS
                     LPRIFEEIVAAWQQRIPAP"
     gene            7408..8472
                     /locus_tag="PKI07_04715"
     CDS             7408..8472
                     /locus_tag="PKI07_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC50387.1"
                     /translation="MRVLLTGICGFVGSTLARGLREGWPDWEILGLDNFARAGSETNR
                     RALRGLGVRLFHGDVRVPSDLETLPRCDWIIEAAANPSVLAGVDGRTSSRQLLEHNLV
                     GTINVLELQKQWRCGFTLLSTSRVYSIRALAAIPVDAQGDRFVPRADTPISGFGARGV
                     TEEFSTEPPLSLYGSSKRASELLACEYAESFNLPVFILRCGVLAGAGQFGRIDQGIFS
                     YWIHSYCRRRPLKYIGFDGAGRQVRDCLHARDLLPLITQQIAGPHAAESRVLNASGGL
                     GQSASLRQLSAWCAQRFGEHPVAAEPRNRPFDVPWLVLDSGRAAQVWGWKPQTSLEAI
                     WTEIAQHAETNADWLEATAD"
     gene            8469..10847
                     /locus_tag="PKI07_04720"
     CDS             8469..10847
                     /locus_tag="PKI07_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOC50388.1"
                     /translation="MRGSPGSSRAAAGGISAAFIVLLLLWLALAGALAAPAPGGGGKG
                     AGPRREPITVVEVALPSRAALDPLVQAGYDVASVRPDRAVVYADDEELANLQAAGWAL
                     KVLVPPASSAAKALGDYHGYTNMTAMLDGYAAAYPALCRKLSLGKSVQNRDLWALKIT
                     TDPDLPQDKPRFRYLAAMHGNEAVGTELCLYLIDLLLKGYGSNDTRIVNLVRNVEIWI
                     VPLVNPDGRESKNPRRYNANNYDLNRSFPEGSGTNLGNGLYGPSINTNGLQPEVRALM
                     AWTTNHNFSLGANLHTGTVVVNYPYDNDNLGSVFSPPPDVTLFQDMARSYASNNPPMW
                     ANNIPPFTNGIVNGADWYAIDGGLQDWSYRYASSLEVTIELSDYQWPNPPASTLPTYW
                     AQNRESMLAYMEWALRGVRGIIRDAQTGQPIRGAVRTEGVHHLVFSDASVGDYHRILL
                     PGNYTLWFYAPGYAPQRVTNVMVSAGAATRLDVALQPVSARFAAKINFQPPAAIVPEG
                     HRADTGAAFGLRAGGYSYGWETALAGGNLIERKAGRSRDLRYDTHCQMQAGGSHTWEI
                     AVPNGPYSVLVAAGDPAYVTGAYRILAENLLLLSGAPSAANRWVEAHGTVIVTDGRLT
                     LSNGPGAVSNRLAFVEINALEPATIEQWRAWWFGTTHNSGVAADANDADHDDLPNFLE
                     YAFGLNPTLPESGWQLSPRVLHTNGTAWLACSFLRNTNATDLAFFLQAAAELPAPSWS
                     NLASWTHADGWVGSAAVSETNAGPGRVCVILTDPQPIEGVPRRYLRLAVDRL"
     gene            10879..12147
                     /locus_tag="PKI07_04725"
     CDS             10879..12147
                     /locus_tag="PKI07_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOC50389.1"
                     /translation="MTTRPITPLRGWVVVSAALGINLILGVLYAWSVMGKALVLQWHW
                     TAAQASLPFTISAASFSLMMVAAGRWQDKIGPRCVASLGGILLGLGLIGSRFATTPGL
                     MLVTFGLLGGVGIGLGYSATTPPAIKWFPPGRKGRITGIVVSGVGLAAVYIAPLTEYL
                     LSVTTIPNTFLILGLGTMVLVTLLSQMLANPPADYEAAPGGDAGAGAATAPAARPNLD
                     WHEMLRTRQFYLLWLMFVMAASAGLMIISQVALIAKDQAHMDKWGFAPVATLAIFNTI
                     GRVVSGFLSDRMGRTNTLILAFVLQAANLFAFSHYTTPGLLIFGSAFTGLCYGTLFTL
                     FPAATADFYGMDNLGVNYGLVFTAFGAAGVIGPMLAGRIRDAYGTCHYAYLISAIMLL
                     LGAGLAMIVKTRSPRAAPPFAGPDQRSRVA"
     gene            complement(12336..14840)
                     /locus_tag="PKI07_04730"
     CDS             complement(12336..14840)
                     /locus_tag="PKI07_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC50390.1"
                     /translation="MAASQAVIWRKRFRLGLSLISGAIFSAFLATGQAQTNYQQLLSF
                     GPTALSGNQPRAKLMEGSDGWLYGTTFSGGISNAGTIFRVGKDGAGFSQIYAFTNSDR
                     PQGGLVEANDGTLCGTTSYGGSNHYGTVFKCNKDGSDFSILHVFPGGAGDGTVPNCTL
                     IKGHDGILYGTTYSGGVSNLGTVFALNTDGSGYAVLHHFTGSSGGADGSSPFSGVCQG
                     LDGALYGTTLMGGSKNEGIVFKVSTNGSGYTILRHFNGGTQDGRWGYGGLVQASNGRL
                     FGTTYYGGTANVGTIFTVNTNGGNYAVLHSFQLDSEGLQPFTSLTMGDNQVLYGTARY
                     GGISDGGIIFRINSDGTGYTVLHRFVPATEDGHQPLAPLLLGSDGALYGSTYLGGVYA
                     TNNVYGTLFRLCSPPTITAQPQDQTITVGQDASFSVSATGTSPLGYQWSFNGTNIPGA
                     TDSSYTCYSAQPADVGNYAVTITNMAGSVLSSNALLTLQGVLTIIAPPTNLAVCAGSP
                     AVFTVEASGLDVTYQWQGSGDGGITFTNLSSTATNASYTNLITTAADDGYQYQVIVSG
                     AFSPPATSAPPAILTVSAPAIADAGLDQTVCASSPATTLAGSIGGAATSATWSGAGTF
                     IPDAATLDAVYIPTPAEVAAGVATVMLTTDDPAGPCGAASDTMTITIMPAATVNAGPD
                     QIVSADNPATTLAGSIGGAATSAMWSGAGSFAPDATTLDAVYTPTAAEIAAGTATVTL
                     TTDDPAGPCEAVSDSMTITITSTAPTPATIISLSGTSLNYTGGSGTQFVLLQSADPTA
                     PLANWTREATNTVTPGVFTVPPVGAAASAFYIIQSE"
     gene            complement(15791..16264)
                     /locus_tag="PKI07_04735"
     CDS             complement(15791..16264)
                     /locus_tag="PKI07_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOC50391.1"
                     /translation="MKTLVTLALGIALGAAAVWFFTTGRHDPRWQSAGKKVEEAAQSA
                     RDAAQDKLRDFRLRPEDLKDELARTGQIIRREVRQAGHAIADATADARITAAIKARLV
                     TSRDLPAFNLSVNTTAGVVTLSGAVSSPEQVGKAILIAMETDGVREVVSTLQVKP"
     gene            complement(16277..17737)
                     /locus_tag="PKI07_04740"
     CDS             complement(16277..17737)
                     /locus_tag="PKI07_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50392.1"
                     /translation="MAVQNRIVKVQKRNRALVKFDDTRIRRAILNAARSIGGFEQDRV
                     PGVNETLFAAYRSEDAIAGFLADAVVVCLNSDPHHLISNFPPTIEIIQDEVLHALRSY
                     GFQNTADAYECYRWGRHWLREGAITPDKFAGNGFPRARMEQTLAWNRQRGCDTVAGLN
                     EIVRRGKIKALVEESLALYEQSLDEAAEKVMRRLASGDQLRMMWVSGPSSSGKTTTTV
                     KLTERLQKRGLRFLMLNLDDYFWSLVEHPTDWINDRNYETPEALDIQLLNGHLTSLLA
                     GETIEKPIYSFKEGRRVASKHIRLEPGQILLLDCLHGLYPPITEGIPAAAQFRLYIET
                     QNVLAEGDGSNGRRTQFTDVRLLRRMLRDVQHRNHSPLLTILHWHYVRSGELFSIIPL
                     SGLADHVVNGGFPFDLPALKPFFAGPDGYLPRPEDFASYTGFLDARIRYERIKHLLES
                     VAGLTREQAASREIIPGDAVIREFIGGSTIKIPHNE"
     gene            17902..21153
                     /locus_tag="PKI07_04745"
     CDS             17902..21153
                     /locus_tag="PKI07_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOC50393.1"
                     /translation="MSGACFHRIGIGRGQGLRARVAGVMAVWLAVAGGAGEFRGLWVD
                     SWSPGLYNAAQISQLVSDARAGHFNALAVEVRRRADAFYNSNFEPKAVGVAEDFDPLA
                     DLIAKAHNTNDGPRLEVHCWIVAYPAWLGSSPPPQPSHPLNLHPDWRNRTSTGAFCTD
                     GQNHYFDPAHPEVQKHIFKVAMDIVSRYDVDGLNWDYIRYPGVLWGYGEVALARFNSR
                     FHRTGWPAASDPDWTAFRRDQVTALVRKVYLSAIACKPQVVLSAAVFTGSPGITNTAD
                     FPSTSAYSSVLQNWLAWMQEGILDLNMPMTYFDHSVSAAAYARWNNFIKDHRYHRQAV
                     VGPAIGQNTISNSLVQIRLTRQTTPAGNRGDGVSCYSYASNNSGRLPIAAFLEALTQP
                     TAHDPLTPPVFSELDVPPDRPWKNSPVRGHLKGFVYDDLGQELDGATIALSGPAIRVL
                     TNDATGFYGAVDLEPGLYTACASFPGHAPATQTVAVVAGMVADLDFSLTPTGAVIVLQ
                     PQDQSALVGSNAVLRARAAGVRPLGYQWLQNGVALAGETAASLTLAHIQLTNAGAYRL
                     VVSNASGAVTSLVATLTTLLPVRVSSARNGTVSLAPNAAGHALNSFVTVAGVPEAGFG
                     FSHWSGDLASTLNPLTFQVTNSLTITGNFAPSPDDLILDNPQASYTGSWTVSQTAEGR
                     YGLNYHYATTTDGPATATATYRPLISRAGAYDVYLWYPAGGNRATNAPWVVSYAGGSV
                     TSLVNQTTDGGKWVQIAGGKYFAAGTNGSVQLANNTGARGKVVMADAVRFVRARGQQY
                     VPIRLEPGSRDVASGFALAVYGNPDQTCLVEASANLRQWAPLSRVRLGSQLAQLVDAD
                     SGAYPHRFYRARQVNVYSLADFENYANGVRVLFGTPASSGSTHEFVDSAAPNFCRVTN
                     SFPAGNASAKVLHIGWSFVAGSSGQSPWLRLATWDAPGLPNPIVSFQQGIGFAVRTDR
                     EIYLAVGLRETSSTGPIGSNGGTVGTLEWIGGTSDNTTDPPRGRRICAGQWIWVSFCI
                     PAEPVRAFVGSGNGVLESSTGKGVFDELVIVPADGLGPYNLYLDNFQFITFEP"
     assembly_gap    21388..21487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21900..22877)
                     /locus_tag="PKI07_04750"
     CDS             complement(21900..22877)
                     /locus_tag="PKI07_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOC50394.1"
                     /translation="MQFDVIIGNPPYQLNDGGGDGSSAIPLYHRFIEQALSLEPRFLS
                     MIVPSKWFSGGKGLDEFRAKMLSDQRIRHLIDYPNSRDAFSGVDVAGGVMYFLWDRDN
                     PGTCMVETRANGQSVIAERSLNEYEVFVRDNRVRGIVKKVKARGEREFSTLVSARRPF
                     GIAAAQAGNKTGELYLYTSSGDCRIDRKDVIKGTELIGKWKVLLSKTSSEHAGQPDKS
                     GRKRVLSRIEVMSPNSVATESYLIIGPFKSEREAEHVAEYLRTRFVRFLVSAILLTQN
                     ITRGSFVFVPAQDFSKSWPDKALYKRYRLTPDEIEFIESTIRPVGESDA"
     gene            complement(23738..24190)
                     /gene="tnpA"
                     /locus_tag="PKI07_04755"
     CDS             complement(23738..24190)
                     /gene="tnpA"
                     /locus_tag="PKI07_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOC50395.1"
                     /translation="MPQSLAQILAHLVFSTKNREAVLADDIRDELHAYCGGIVANQQG
                     TLLKAGSVADHIHLLIAMPRTCAPADLVQEIKTGTSKWLKTKGLRYARFHWQSGYGIF
                     SLSPSHRAAHAKYIGEQAEHHRAMTFQEEYRRWLKKYNVAYDERYVWD"
     gene            complement(24462..25106)
                     /locus_tag="PKI07_04760"
     CDS             complement(24462..25106)
                     /locus_tag="PKI07_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOC50396.1"
                     /translation="MALVKSKQRVADHGEVFTPAWMVEAMLDLVKGETERIDSRFLEP
                     ACGNGNFLVQILRRKLAAVELKYGKSDFERRHYALLALMCVYGIEILPDNIADCRANL
                     REIFAEYLNLEPSDDFYRAAVCVLSVNLIHGDAMKMRTWDNLPITFAEWAYLWKGRYQ
                     RRDFSLQSLTLSSAFSAQDSLFAQLGKHEIFTPTKTYPPMTVSELIARTPQATL"
     gene            complement(25394..25471)
                     /locus_tag="PKI07_04765"
     tRNA            complement(25394..25471)
                     /locus_tag="PKI07_04765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25434..25436),aa:Pro,seq:cgg)
     gene            complement(25590..26978)
                     /locus_tag="PKI07_04770"
     CDS             complement(25590..26978)
                     /locus_tag="PKI07_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOC50397.1"
                     /translation="MGKEFSITPRHVARVETRHRRIVTPLPHPDSVPTLEKLRQFEPQ
                     SMRGQPPLVWDRAEDVFVYDAYGNRWLDWSSGVLVTNAGHCAPEVRQAILAQVNHGLL
                     HNYVFPSEVRAALVEQLIQVAPPGLEKVFLLTTGSEATECALKLARAHGIRKGGPRKI
                     GLVGAERGFHGRTLGAQQAGGIPGQKSWIVNEDPAIVQVPFPDGYWTEDTRFELFQAT
                     LARKGLVPENVAGVIFETYQGVGPDFAPVDYIRQLAQWCRAHEALLIFDEVQAGFGRT
                     GRFWAFEHYGVTPDLICCGKGISSSLPLSAVIGRSEIMDQFGPGSMTSTHTGNPVCCA
                     AALASVRKIVAENLTANAARLGEILAAGLRAIQQRHAQVIGHYSCRGLVGGLQITQPG
                     RKEPNHDLAHDIIERCFHKGLLFFAPVGAWGQTVKIAPPLTIREDALREGLAVLSEAV
                     DEAVAAPAAREK"
     gene            27201..28112
                     /gene="nadE"
                     /locus_tag="PKI07_04775"
     CDS             27201..28112
                     /gene="nadE"
                     /locus_tag="PKI07_04775"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOC50398.1"
                     /translation="MKTAQQRIKLARMDPEQVADEIGGFIVEAVRRLRKTGAVLGLSG
                     GVDSTCVAALAKRAFDRHNAQSPDQPLELAGYILPSRTSEARDVEDARQVATRLGLRH
                     EVQSIEPIVEAFQTTNPEALRHAYHRGNLTSEIRAVILHAKAATENKSVIGTGNRDED
                     YGVGYYTLFGDGAVHLSPIGNLPKRLVREMAAHLGFQDLAGRVSSAGLEPGQTSFKDL
                     GCDYEFAEVILNGLDQGLRAEELSAHPQVVPYARQQIEKYTQWYGAPKFASVPELVAE
                     VLRRHELARLKVRLVQPDIAPITLTLE"
     gene            complement(28153..29418)
                     /locus_tag="PKI07_04780"
     CDS             complement(28153..29418)
                     /locus_tag="PKI07_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOC50399.1"
                     /translation="MQNLYDYQRFAILYVDDEELSLKYFTRAFRDQFRIFTAPNAQEG
                     LKLLEAHKDEIGLLMTDQRMPGEKGIWLLEKARQLRPRIIRILATAYSDMEVAIAAVN
                     TGAIYKYVTKPWDVPQLENTLQRGLEFFMVQRERDQLLKEKMSVLHNMMIADRIVSLG
                     LLAAGLSHHIRNALVAVKTFLDLAPSKMAEEKLDMESLRNPDFWKEYYQNVQGQIEKI
                     SHLLKDLWTASEKPTYEFANRAHLHEVVAGVIASLKDAFAGKQIVVENHIPDSLPALN
                     VDLPKFTRLFDLLLRDELVSLSAGNRVTLTAKLLDAGPPGQPEVLVELNDDGPGLPQE
                     ALRLVFDPFVLRSDSPAEYGIHLMACYFIVHHHGGWIDARSEPGRGTTFRLRLPLDPN
                     RAASPQTEESFLQKVLLNETLWEKLISSD"
     gene            complement(29441..30802)
                     /locus_tag="PKI07_04785"
     CDS             complement(29441..30802)
                     /locus_tag="PKI07_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC50400.1"
                     /translation="MADGAPHNREIEAAFAEYDRRQTISNFKVACVIGMVLMPAGVII
                     DHAVYPALAAQFLKLRLENAVLIGAFLALLLTPLGHKYYRFLGITLFMLPTSCMAYMI
                     KVSPDGAASPYYAGLNLVLLVLAFVLHWTFRESLCAAVLVLGLYLAAVFFNQGPVAWH
                     LFVNNVYFLGLTGIIVATGSYFHSKIRFGEFAFRYQLDRQNRILEATIQQLKETELQL
                     VQSEKLASLGRLSAGIIHEINNPLNFTATGLFTLRKKSRHLPPAEQEEYADVLKDVED
                     GVQRVKDIVSSLRTFTHPETESRDAVEVAEVVTAALRFLSNEWKGKVRIESKLAERQT
                     VWANKNLLTQALLNLLTNSLDALKTKTFAGEPPAIQIEGCLEPGKSILRVRDNGPGIG
                     AEHLDKIFDPFFTTKDVGQGMGLGLATCHTIMQNCGGRISARSEPGQFCEITLEFPAP
                     GTP"
     gene            complement(30795..32213)
                     /locus_tag="PKI07_04790"
     CDS             complement(30795..32213)
                     /locus_tag="PKI07_04790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC50401.1"
                     /translation="MKNGASTENLVLGASRQAGELRARLRRLTRYSAVLEIYQPGLVL
                     QLSEVLEGFQIILQGRVIYEGRAVVTALINAGTMLIGEVRLDEGSFRLSSFSPGEDPA
                     PLHEGFQAFLGHWQKLYRVRPAFKVVVADMQTFLSDLRLWLEQTEVEIRAARPGARAE
                     REEAAAREIGRDMVPAFDAMHERLESIAADLEPELRPVHQVFAQRLLHPLVLCAPFAH
                     RTYHKPLGYAGDYEMVNMILRDPHEGGSLFAKVVNRWFLSQWPARAHRNRIQYLKGLL
                     REEARRRATRGQPLRVLSLGCGPAREIQEFLAEDALGHPARFELWDFNEETTRHVQRV
                     LEDCRRRHGRRVELDIRRQSVQQVIKEGARAAAAPVREYDFIYCAGLLDYLSDRIAAQ
                     VMAGLYDWLAPGGRMAATNVEDCKPFRHMLEFVLDWHLFYRNPAQSAALLPPGLSAAR
                     TRVLKDPTEVNVFVEARKPEHG"
     gene            complement(32372..33709)
                     /locus_tag="PKI07_04795"
     CDS             complement(32372..33709)
                     /locus_tag="PKI07_04795"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HOC50402.1"
                     /translation="MNWPSSSALPWSMHHSGQGTVLDSDAALLAGADAQPASFIPVAR
                     RERADGWIRRAPKAGVRGAEAASPAQGAGIRVLEGESARALDSRQKWDLLSPFLRQHG
                     REGIAYATLQSGMEYFIGPSGYIAYTRARHPLWARRPRRIVFSDPICAPEDVPALVGS
                     FLAQDRHAAFGVISEACARALRPLGFKVNCIGYEPLMAVQTYNTEGNWKELDLIKRAR
                     NEARREGLTIREESGMSLKQRRRELKAFSARWVRSRKINDREIWLYARRPVFEPEEGV
                     RKFVAYDPERRVAGFAFYDPMYRGGRVFGYSANIVRCDERRFGRLATALHMVAMETFR
                     AEGVEVLNLLLAPFGKLECGAFNDDWAARQLFRWSARFGNGLYNFQGLSFHKSKYRGA
                     ERPLYFASNGWLPCADVYLAFASAGIADGCWGMLRKLLRGWRAAGGGRGGPPA"
BASE COUNT         6047 a        10582 c        11008 g         6407 t
ORIGIN      
        1 ttattgcgcg aacctcacgc ccggcggcgc acccgctgga agaaaaccag ctcgcaccac
       61 cccgaaaggg cctcctttcg caagcggcaa accgcgccgg aatagccatt tccaacccgc
      121 ctacccccgg caccttgacg ggcgcatccc ggcagggtat ccgccgggat tctcaaaaat
      181 gagaaaatgc ccctgccccc tcccccgccc gccgccgcgc ctcccgccga cctttcctcc
      241 tcccatatcc ccatttccgc caatttcccc ctcctttccc gccgtgtaca aggggtgtac
      301 aagggatgct caccggaagt acatcgtttt ttcctatgtt cacccggtgg gcatcccttg
      361 tacacccctt gtacacgacg ggaaaggagc ccgggagagg ggggataggg cggttttgga
      421 gcaggattgg ggggtggccg taagggaaga ctgccccgcc ttggatcggg tttgggctgg
      481 ctggggggct tagcccggcg gtttacccgg tttggacggt ttcctggcgg agggaggggg
      541 cgccggttct ttgagccggg ggaataccag gcaaacgagc aataagacgg tgacggccag
      601 cgccgtcagg ctgacgtata ggatgcccgt cgggggggcg aagcggaact cgactgtatg
      661 gctgcctggg ggcaggtaaa cgccgcgcat gaggtaattg caacggagca gtttcccggc
      721 tttgccatcc acggatacct gccaattagg gtcgtatttg tcattgagca gcaagaccgc
      781 gggcgtttcg gctttggctt gcagcacgat ctgtttgggg gcgtagctgg caaactcgac
      841 cgtgccgggg ttcgggttgg tcgcattgag ggggtcgggg gcgggcagcg ggtcggccag
      901 gagcacctgt tgggcggggt caaactcggc gctgcccagc gtggtgaggg tggcctggtc
      961 gttggtgctg acttgccagt ttgcgtagag cttcgcccgt ggcaggacgc cggtaaattc
     1021 aaagacggca taggggccgt cgggcttgat caccgcggtc agggtctcca gggtggtggc
     1081 ccggtctatg ccgggtttcg tttcaatatc gaaccgggtg gcgatgcgga agcggttctg
     1141 ggccgggtcc agttcccggt tgagcgcgct gagatagctc gccgcgccga gcaggtagcg
     1201 ggtgttggta agctgccagc ggcgcgccac ccgatgccgc gtgccggggg tgccgtcgga
     1261 gcccagggcg gattcatagg cgaggaactc gaccggcgcg cgcggcatca tgacgatgtc
     1321 cagcgactgg atattgtagt aggggaagag ctgctgcttc cactcgattt cgtaaagctg
     1381 gtggaacagg gcatattccc ggggcgtgac aaacggcagc accgcgaccc ggtgctcgta
     1441 aggtttgtcg cgcaagaggt caatcaccgg gttggtggcg tatttttgtt gccagttcca
     1501 ggagatgacc cagggcatgt tggcccagcc caggtcggcg accagcagga cgcccagcag
     1561 gattccgccc agccgcgccc gccgtccgct gaagtagccg ctgacgaccg cgagcatcag
     1621 gcccaaagcg agcgtgaaca ggaccaggaa cacgccggcc tggcggatgc tgaagccggc
     1681 gatctcgcgg gccatgcccg cgtcaaactg aacttcctgc aaataggttt ccagggacgg
     1741 gcgcgattgg gcgtagatca gccaggcgag cccggcggcg gccagggcga gggcggagcc
     1801 ccgggcccag tttttctcga agcggccggc ccgggcccac caagtgcgca attgcgcggg
     1861 cagctcgcgc aaggctgcgc cgggggcatc caggaacgac cggctgaggc catgcaggcc
     1921 gcaggcgaac aggatgacca gggcccagtc cacgacgtgg aaaaacttgg cggggttgcg
     1981 gatggtggag gcgaacggca gggcgtaaaa gaattggtag aaaggcgcga agcggcccag
     2041 gccaaccagg aaggcgatga ccgtcacgcc gctccagaac ccgatgaact ttcgctcccg
     2101 ccgcgagaac accgattgct cgcgccgcag cgcctgcagc attgtccaca gcgcaaccag
     2161 caccaccagc gttcccgcat acacgccgcc gccgccatag cgaatgaagc cggagggcgg
     2221 gggccccggt ttgccggcgg cgaagtagcg gtcccactcc gggtggcgtc cgccggcgcc
     2281 ccagtagcag cctcccaggt aatagttttg caggccctcc ggcaggttca tgggggtatc
     2341 catgcggtag ccaaacaagc cgggcacaaa caggctgagc gtttcgcgtt tgggcatgct
     2401 ccactgggtg gcggcatccc agcgctgggc tttgctggcc tggtcctggc ccatgccggc
     2461 cacgcccttg atctgggtgc cgaccagggt gctgacggcc gatgcggcga cgaatgccgc
     2521 gaacgcggcc accaccgcca gccgccccac gccccgggcc gcgcgctgcg cccgcgtgcc
     2581 ttcgccggcc cacgcctgcc agagcacaaa gcccgcaatg accaggctga aaatggcccc
     2641 gatgtcaaag gcttccatca cgcccaggcc gacggccagg ccggccagag cggcgcggag
     2701 ccagcgccgg ggcgaggaga ggtcggccag cgccgccagg gccaggaagt ccagcccgaa
     2761 gctcaggggt tgcgcggcca cgccccagca ggcggtggaa aagaagtccg agttcagcgc
     2821 cacggcgagg ctgcccagga aacaggcccg gggggagaac ttccactggc ggaagcacag
     2881 ccaggcgctc aggccgagaa acagcagcgt gaacggcgcg tagattttcg aaaagagcag
     2941 cggcccggcc agcagcgcca gcccttgcga gatgtccggc gaagcgctgg gccccgtgcc
     3001 gccgagccag ttgaggtcgc cccagtagct gaagaagccc gcgggcaacc gggcgctctg
     3061 cgagctgatg gcgccgagcg ggccgtcgtt ggaaaagacc gtgtggcccg ggcgcaggcc
     3121 atcccggaag agcacggcca gcaacacggc caggcagagc gcgatccaca ggaactgccg
     3181 tgcggccgaa ggcggaacgc cggccccgct tttcgattga ttcatagcgc cgaagtgttc
     3241 ttcatttccg ggtgaaagaa aatagaaatc cgccccgatg aacaagccgc aagcagccgg
     3301 gcccgggggg ggagtctttc ggagcgggtg gttttgggcg gccagcctct tcctggctgg
     3361 cctgggggcg cggctgtggc tcattgaccg gtttgggacg gcgctgccgt tttgggatca
     3421 atgggaggag gcgcgcgtgg tgtatgtgcc gttcctggcc gggaaattgt ccctggcgga
     3481 cttgttttcc gcgcataacg agcaccggat actgttcacc cggctttatg ggctggcgct
     3541 gctgctgttg aacgggcagt gggatgggcg gctgcaaatg gtcggcaacg ccctgatcca
     3601 cagcgccacg ctggccgggt tgggggggct gatgttccgc tggctggagc ggaaatactg
     3661 ggtgtgggtg tggctgccgc tggcgctggt gctggcgctg ccttttgggt gggagaactc
     3721 gctggccggg tttcaatcgc agttctactt tctggtgctg ttctcgttgc tcacgttgtg
     3781 gctgctgggg ctgcatccgc ccggctcggc gccctggtgg tgcggcgcgg ccacggccct
     3841 gatggccctg ttcacggttg cctcggggtt tctggcggcg gcggcggtgt tcgcgctggt
     3901 gctgttgcgc ctcgggcggc agcgggaggg ctggaggcgg gaggcggtga ccttgctgtt
     3961 ttgcgcgggc gtgtcgctgg ccgggctggc gctgaagacg gacgtgcggc atcaccaggc
     4021 gttgcaggcc cattccctcg gcgacttcgt ggctgcgctc ggcgccaacc tggcgtggcc
     4081 gtggatagtg gttcctccct ttgccgtgct gaacctgctg ccgctcgtgg cgcttggcgg
     4141 gttttacctg ggggcgcggc gggcgcggca cccggcggag gaaatgaccc tggcgctcgg
     4201 gctttggacg gtgctgcagg gcctggccac ggcgtatgcc cggggcgcgg aagggaagcc
     4261 gccgggctgg cggtatatgg attcgagctg ttttattttg gtggcgggtt gttttagcat
     4321 cgccatcctg ttgagccgtt acctggcggc ggcgcgggcg cggcgggccg ggcaggtgat
     4381 cttcggcctt tggggcattg gctgcgcggc ggggttggtc ctgttgacga agcgcgcctg
     4441 gcaggttgat atcccggagc ggcaattcta cttccgctgc cagcttctga atacgcgcgc
     4501 tttcctggcc acgcgggacc ttcgggtttt ggaccacaaa ccgaacccgc agcttgcgct
     4561 ctacgaggga gatccctacg cgccccgccc ggcgcatgcg ggtgcaaagc tggcggcata
     4621 cctcgattac ccgggcgtcc gcggctttct gccggcgtgc gtgcgcgacc cgttaacgct
     4681 cgttcccctg cataaccacg gctttgcgac caatggcgcg gcgcgcgccc ggccgcgcat
     4741 cccgggagag gtctcctggg gctccgacac ggaggcgggc gcggcggcga cggggagttt
     4801 cgagagcgcg ccgatccccg ccagcaagcg gcctttcctc gaattcaggg tggccggcga
     4861 cctggggaaa ccgggcttgt ccctggccgt cgtcgagctg agctccggcc aggcccgggc
     4921 cgtgcagccg cgcccggcgc cggggaacac ctggcgaacc tgccaggtgc gggccccccg
     4981 cggggcgttc aaggtggtgg cgcgcgatgc gagcgcggac gggtggttcg cctttcaggc
     5041 gccgcgggaa gtgggcgggt tatcgtgggc ggcggcgcgg ctggccgccc tgggcggcgg
     5101 gttgttcttt gccggcctgg gcttgggcct gctggggatc gtgggaggcg ggcgttttcg
     5161 tccgggcgcg gattcttccc ggcgggcggc gggcgacgga ccgccgtccc agtcggccgt
     5221 ttgaagggcg gcgtggacat ccggcgggag agtccggctc gcgcggccgc cggcatttcc
     5281 gcggcggtcg cgacgccgcg ggttcagcgc ggtttccggt aatcgccgcg gctgaaatac
     5341 ttttcgagcc agacgtagag gcagatgaac aggtagcggc tgcccatctc ccgcagcttc
     5401 agcttggcca ccccggtgcg gcggttgcgc caggtgatgg ggatgaccgt ccaggaatat
     5461 ccccggacga tcgccttgag cggcaactcc acggtcaggt tgaaatgcgg ggagatgagc
     5521 ggccggcatc cgtctatgac ttcgcgccgg taggctttga aggcgttggt ggtgtcgttg
     5581 aggcggatgc caaagagcat tttcaggaac aggttggcca tccggttgat aagcagcttg
     5641 attttggggt agtccaccac gccgccgccc ttcataaaac ggctgccaaa cacacaatcc
     5701 caaccctggt tcaactcctg ccagtagcgc accacgtcgc ggcaatcgtc cgattcatcc
     5761 gccatcatga ttaccacggc atcgccctgg aggtggtcaa gcccgcggat tacggcccgc
     5821 ccaaaaccgt gcaaaccttc attccggagc ggtttcagct ccggcagccg tgcctgcagg
     5881 tcgcacagca gcttccaggt tccatcggtg ctgccgtcgt ccaccaccac gatttcatgc
     5941 ggaatgttgc gcaagcgcaa ctccaggtgg aggtgttcca cggtcgagcc aaggcagccc
     6001 tcttcatcgc gcgcgggaat gaccaccgag agcagcgaca gcgggcgcgg cgggcgtgga
     6061 gcgggagtgc tcatgccggg cgggaaagcc ggctggccgg cggcccgtcc ggacgcgcgc
     6121 gcccgccggc tcgggcggat cggcgggagg ggccgcagcc tgggatgatc atcattttcg
     6181 ccatgcgaaa cgctggccgg aagtgtaatc cggaagccct gcgggacaag ctcattccgc
     6241 cgccggagct ttctgtttgc ggccggcccg ggcggaaggg gccttgggga ttcgggtcaa
     6301 aggggcttga cctggggcgg gcaggctgtc ttgatggagc cgcatgagca aagcgtgtct
     6361 ggtcacaggt tcgtccggct tgatcggatc ggaagtcgcc gcctttttcc atgagcgggg
     6421 ctttggggtt cacggcgtgg acaacaacca gcgggaggcg ttcttcggcc cgcaaggcaa
     6481 cacgcgctgg aaccaggagc ggctgcaacg gaccctggcc gggttcaccc atcacgaggt
     6541 ggatattcgg gatcgcgccg ccgtgctgga gttggtcaag acgcttcagc ccgccgtcat
     6601 catccacacc gcggcccagc cgtcgcatga tttggccgcc cggattccgt ttgatgattt
     6661 tgatgttaac gccaccggca ccctgaatct gctcgaagcg gcgcggcggt attgcccgga
     6721 atcgcccttt attcacatgt ccaccaacaa ggtgtatggc gaccggccca atacccttgc
     6781 gctccaggaa cgggagacgc gctgggacta tgccgacccg cagtttgcgc aggggatacc
     6841 ggagagcttt cccattgacc agtccaagca ctcgctgttt ggcgcgtcca aggtcgccgc
     6901 cgacgtgatg gtgcaggagt atgggcgcta cttcgggctg cccacctgct gcctgcgcgg
     6961 cgggtgcctg accggcccga accatgccgg ggtggagctg cacgggtttc tgagctacct
     7021 ggtgaaatgc aatctcgagg gccgcgaata ccggatttac ggctaccagg gcaagcaggt
     7081 gcgcgataat atccattcgg tggatgtggc ggggttcatg ttcgagtttt accaggcgcc
     7141 ccgggcgggc gaggtctata acctcggcgg cggcaaggac aactcctgct cgatccggga
     7201 agcctttgcg atggcggagg cctgcaccgg ccggaagcaa gtccacacct acgtggagca
     7261 ggcccgcagc ggcgaccata tctgctacta cagcgatctg cgcaagatgc gggcgcacta
     7321 tccccgctgg caactgaccc gcagcctgcc gcggatcttc gaggagatcg tcgccgcctg
     7381 gcagcagcga atccccgctc cctagaaatg cgcgttctcc tcaccggcat ttgcggtttt
     7441 gtgggcagca cgctcgcccg cgggctgcgg gaagggtggc cggactggga gatcctcggc
     7501 ctggacaact tcgcgcgcgc cggcagcgag accaaccgcc gggcgttgcg cgggctgggc
     7561 gtccggctgt ttcatggcga tgtccgcgtc cccagcgacc tcgaaaccct gccccgctgc
     7621 gactggatta tcgaggcggc cgccaatccg agcgtgctgg ccggtgtgga cggccggacc
     7681 agcagccgcc agttgctcga gcataatctt gtgggcacga tcaacgtgct ggagctgcaa
     7741 aagcagtggc gttgtggctt taccctgctc agcaccagcc gggtctattc gattcgcgcg
     7801 ctcgcggcca ttccggtgga cgcgcagggc gaccggtttg tgccgcgcgc cgacacgccg
     7861 atttcggggt tcggggcccg gggcgtcacg gaggagttct ccaccgagcc gccgctgtcg
     7921 ctctacggca gttccaaacg cgcttcggaa ctcctggctt gcgagtatgc cgagagcttc
     7981 aatttgccgg tgttcattct ccggtgcggg gtgctggccg gcgcgggcca gtttggcagg
     8041 attgaccagg gcatcttttc ttattggatt cacagctatt gccggcggcg gccgctgaag
     8101 tatatcgggt ttgatggcgc cgggcgccag gtgcgcgatt gccttcacgc gcgtgatttg
     8161 ctgccgctga tcacgcagca gatagccggg ccgcacgccg ccgagtcgcg ggtgctgaat
     8221 gcgagcggcg gcctggggca aagcgcttcg ctgcggcagt tgagcgcgtg gtgcgcgcag
     8281 cggtttgggg agcacccggt tgcggccgaa ccccggaacc gcccgtttga tgtcccctgg
     8341 ctggtcctgg attccggccg ggccgcccaa gtttggggct ggaaaccgca aacctcgttg
     8401 gaggccattt ggaccgaaat agcccagcac gccgagacga acgcagactg gctcgaagcc
     8461 accgccgatt gaggggaagc ccaggttcct cccgggcggc ggccggtggc atttcagcgg
     8521 cgttcatcgt tctcctgctc ctttggctgg cgctggccgg cgccctggca gcgccagcgc
     8581 cgggcggagg cggcaaaggg gccggcccgc gccgggagcc gattacggtg gtggaggtgg
     8641 ccctccccag ccgggccgcc ctggacccgt tggtgcaagc cggctacgac gtggccagcg
     8701 tccggcccga ccgtgcggtt gtgtatgcgg acgacgagga gttggcgaac ctgcaggctg
     8761 cgggatgggc gttgaaggtt ctcgttcctc ccgcgagttc ggcggcaaag gcgctcgggg
     8821 attaccatgg ctacacgaac atgacggcca tgctggacgg ctacgcggcg gcttaccccg
     8881 cgctctgccg caagctgagc ctgggcaagt cggtgcagaa ccgcgatctt tgggcgctga
     8941 aaatcaccac ggatccggac ttgccccagg ataaaccccg gttccgctac cttgccgcca
     9001 tgcacggcaa cgaagcggtc ggcaccgaac tgtgccttta cctcatagat ctgctcctta
     9061 aaggctacgg cagcaatgat acgcgcatcg tcaatctcgt ccgcaacgtc gaaatctgga
     9121 tcgtgccgct ggttaatcca gatggccggg agagcaaaaa tccgcggcgc tacaatgcga
     9181 acaactacga cttgaaccgt tcgtttccgg aggggtcagg cacaaacctc ggcaacgggc
     9241 tttacggccc gtccataaac accaatggcc tccagccgga ggtgcgcgcc ctcatggcct
     9301 ggaccaccaa tcacaatttt tcgctcggcg ccaacttgca taccggcacc gtggttgtga
     9361 actaccctta cgacaacgac aacctgggca gcgtattcag tcctcccccg gacgtgacgt
     9421 tgttccagga catggcgcgc agctatgcgt ccaataaccc gccgatgtgg gccaataaca
     9481 ttcctccctt caccaacggc atcgtcaatg gcgcggactg gtatgccatt gacggcggcc
     9541 tgcaggactg gagctatcgt tatgcctcct cgctcgaggt gaccatcgaa ctctcggact
     9601 accaatggcc gaacccgccc gccagcacac tgccgaccta ctgggcgcag aaccgggaat
     9661 ccatgctggc ttacatggag tgggcgctgc gcggcgtgcg gggcatcatt cgcgatgcgc
     9721 agaccggcca gcccatccgg ggcgcggtgc gcacggaagg ggttcatcac ctcgtgtttt
     9781 ctgatgccag cgtgggcgat taccaccgca tcctgctgcc gggcaattat acgctgtggt
     9841 tttacgcgcc ggggtatgcg ccgcaacggg tgacgaatgt catggtcagc gccggcgccg
     9901 ccacccggct cgacgtcgcc ttgcagccgg tcagcgcccg gtttgccgcc aaaatcaatt
     9961 tccagccgcc cgcggccatc gtgccggagg gtcatcgcgc cgatacggga gcggcgtttg
    10021 gcttgcgcgc gggcggatat agttatggct gggaaaccgc gctagctggc gggaatctca
    10081 tcgagcgcaa ggctggccgt tcccgggacc tgcgttatga tacccattgc caaatgcagg
    10141 ctggcgggag ccacacgtgg gaaattgcgg tgcccaatgg cccctactcg gtcctcgtcg
    10201 ccgccggcga tcccgcctat gtcaccggcg cttatcggat cctggcggag aaccttctac
    10261 tgcttagcgg cgcgccctcc gccgccaacc gatgggtcga ggcgcatggt accgttatcg
    10321 tgacggatgg aaggctcact ttaagcaacg gccccggggc ggtcagcaac cgcctcgcct
    10381 ttgtggaaat caacgccctc gaacctgcca cgatcgagca gtggcgcgcc tggtggtttg
    10441 gaaccaccca taattctggt gtggcggctg atgcgaatga cgccgatcat gacgatttgc
    10501 ccaactttct ggaatatgct ttcggtttga acccgaccct ccccgaatcc ggttggcaat
    10561 tgtcgccccg agtgctccac accaacggca ccgcctggct ggcctgctcc ttcctccgca
    10621 ataccaatgc gaccgacctg gcctttttct tgcaggccgc cgctgaattg ccggcgcctt
    10681 cctggtctaa tctcgctagc tggacccatg ccgatgggtg ggttggttcg gcggctgttt
    10741 ccgagacgaa cgcagggcca gggcgcgtgt gcgtgatact aaccgaccca cagccaatcg
    10801 agggggtgcc gcgccgctac ctgcgcctgg ctgtggatcg cctctgatca tgcccgcccg
    10861 cgaggcccga aagctcccat gaccactcga cccataacac cgctgcgcgg atgggtggtg
    10921 gtgagtgcgg cattgggcat caacttgatc ctgggcgtgc tgtatgcctg gagcgtcatg
    10981 ggcaaggcgc tggtcctgca atggcattgg actgcggccc aggcctcgct gccctttacc
    11041 atttccgccg cttccttttc gctgatgatg gttgccgcgg gccgctggca ggacaagatc
    11101 ggcccgcgct gcgtagcttc gctcggcggc attttgctcg ggttggggct gatcggttcg
    11161 cgcttcgcca cgactccggg actaatgctt gtcacctttg ggctgctcgg cggcgttggc
    11221 atcgggttgg gctactcagc cacaaccccg ccggccatta agtggtttcc tcccggccga
    11281 aaagggcgga tcaccggcat cgtcgtgagc ggggtggggt tggcggcggt ttatatcgcg
    11341 cccctgacgg agtatttgtt aagcgtcacc accatcccaa acaccttcct gattctcggt
    11401 ctgggaacga tggtgctggt gacgctcctg tcccaaatgc ttgccaatcc gcccgccgat
    11461 tatgaggcgg ctcccggtgg cgacgcgggc gcaggggcgg ccacagcccc ggcggcacgc
    11521 cccaacctcg attggcatga aatgctgcgg acccggcaat tttatctgct ctggctgatg
    11581 ttcgtcatgg cggcgtcggc gggattgatg atcatttcac aggtggccct gattgccaaa
    11641 gaccaggccc acatggataa gtggggtttt gcgcccgtgg ctacgctggc gatctttaac
    11701 acaattgggc gggtggtcag cggattcctc tccgaccgca tgggccgaac caatacgctg
    11761 atcctcgcct tcgtgctgca ggcagcaaat ttgttcgcct ttagccacta taccacgccc
    11821 ggcctactga tcttcggctc ggcttttacc ggcctttgct acggcaccct cttcactttg
    11881 ttccccgccg ccacggcaga tttctacggc atggataacc tgggagttaa ctatgggctg
    11941 gttttcaccg cgttcggcgc ggctggggtg atcggtccca tgcttgccgg ccggattcgc
    12001 gatgcgtacg gcacctgcca ctacgcctac cttatctcgg ccatcatgct cctgctcggc
    12061 gcaggcttgg cgatgatcgt caaaacgcgc agcccgcgcg cggcaccgcc gtttgccggt
    12121 ccagaccagc gctcccgggt ggcataacgc aattgcgcca gttcggcagc ccaggcgctg
    12181 aagcgtgtcg ccaactcagc taaccagact ccagatttgg gtgacgcagg tcgcttcgcg
    12241 atttattaag gcgcttcggc ccagactacg aaaaaggccg ggtttattgc cccggcccgg
    12301 aaaccgagag gcttcgttta gaaagcgaac ggttattatt cgctttggat aatatagaat
    12361 gcggacgccg ccgcgcccac cggcggaacc gtgaaaactc ccggggtaac ggtgttggtc
    12421 gcttctcttg tccagttggc aagcggcgct gtcggatcgg cggactgcaa gaggacaaac
    12481 tgtgttccgg aaccgcctgt atagttgagg gaggtgccgc tgaggctgat aatggttgcc
    12541 ggggttggcg ctgtggacgt aatggtgatc gtcatcgaat cgctcaccgc ctcgcacggc
    12601 ccggcgggat catccgtcgt cagggtcact gtggcggtgc cggccgcaat ttctgccgcc
    12661 gttggcgtat aaaccgcgtc cagggttgtg gcatccggag caaagctgcc ggccccgctc
    12721 cacatcgcac tggtcgccgc gccgcctatg gagccggcca gcgtcgtcgc gggattgtcc
    12781 gccgagacga tctgatccgg ccccgcatta actgtggcgg ctggcataat cgtgattgtc
    12841 atcgtatcac tggccgcacc acagggcccg gccggatcat cagtcgtcag catcaccgta
    12901 gcaacgcccg ccgcaacctc tgcgggggtg gggatataaa ctgcgtccag ggttgcggca
    12961 tccgggataa aggtgccggc cccactccat gtcgcactgg tcgccgcgcc gcctatggag
    13021 ccagccagcg tcgtcgcggg actactggcg caaaccgttt gatccaaacc ggcatcggct
    13081 atggcgggcg cgctaactgt cagtatggcc ggcggcgcgg aggttgccgg cgggctgaaa
    13141 gcgccgctga ctatgacttg atattgatag ccatcgtcgg ctgccgtggt aattagattg
    13201 gtgtagctcg cgttggttgc ggtgctgctg aggttggtaa acgtgatgcc gccgtcgccg
    13261 cttccctgcc attgataggt cacgtccaaa cccgacgcct ccactgtgaa aaccgctgga
    13321 gacccggcac aaacagccag gttggttggg ggggcgataa tcgtcaaaac cccttggagg
    13381 gtcagcaggg catttgaact gaggacgctg cccgccatgt tggtgatggt aacggcgtaa
    13441 ttgccgacat cggctggctg tgcgctatag caagtgtagc tgctgtccgt tgcgccgggg
    13501 atgttcgtgc cattaaacga ccactgatac cccaatggcg atgtgcccgt cgcgctcacc
    13561 gagaaactgg cgtcctggcc gaccgtaatg gtctggtcct gtggctgcgc ggtgatggtg
    13621 gggggagaac acagcctgaa taacgtgcca tacacgttat tggttgcata tacccccccc
    13681 aaataagtgg aaccataaag cgcaccatcg cttcccagta agagcggcgc cagtggttga
    13741 tgcccatctt cggtcgcagg gacaaatcgg tgcaaaacag tatacccggt cccatcagaa
    13801 ttaatgcgga atatgatgcc cccgtccgaa atgccgccat aacgcgccgt gccgtacaag
    13861 acttggttgt ctcccatcgt caaactcgta aacggctgaa gcccctcgga gtccaactgg
    13921 aagctgtgca gcacggcata attgccgccg ttggtattaa cggtgaaaat cgtgccgaca
    13981 ttggcggtgc cgccatagta agtcgtgcca aacaaccgcc cattgcttgc ttgcactaaa
    14041 ccgccataac cccagcggcc atcctgcgtg ccgccgttga aatgacgcag gatggtgtag
    14101 ccagagccgt tggtgctgac cttaaaaacg attccttcat tcttgctgcc tcccatgagc
    14161 gttgttccgt aaagcgcgcc gtccaagcct tgacaaaccc cggaaaaagg agaactgcca
    14221 tccgcaccac cgctgctgcc tgtaaagtga tgcaaaaccg cataaccgct cccatcggta
    14281 ttcagggcaa aaacggttcc caggttggag accccgccgg aataagttgt cccataaagg
    14341 atgccatcat gccccttaat caaggtacag ttaggcactg ttccatcccc agcaccgccg
    14401 ggaaagacgt gcaggattga gaaatcgctg ccgtctttat tgcatttgaa caccgtccca
    14461 tagtgattgg aaccaccgta tgaagtggtg ccgcacagcg tcccatcatt cgcttccacc
    14521 aggcctccct ggggacgatc gctgttggtg aaggcataga tttgcgagaa accggctccg
    14581 tctttgccaa ctctaaaaat ggtgcccgca ttgctgattc cccccgaaaa ggttgtgcca
    14641 tagagccacc catcgcttcc ttccatgagt tttgcccgcg gctgattgcc cgacagagcc
    14701 gtagggccaa aagacaggag ttgctgataa ttggtttgtg cctggccggt ggccaggaac
    14761 gcgctgaaaa tagcgccgga aataaggctt aatcccaagc gaaacctctt cctccagata
    14821 actgcctggc tggcagccat gagcaatcgg ccgggcgagg ctcgcccagc actgtcacgc
    14881 caggggcaat ggcaaattga ggataccttt agcgctgaac tctttatcat acgtggcatt
    14941 cgatttatcg taatttcaaa accactctaa ttcacaattg aattcccgca actggtggct
    15001 tcccggaaga tgctcccaga gaatgagcga aagccaagcc cggggattcc ccgggactaa
    15061 accgggagat ggcggcgtga gcgcgtacta taaaggactt tgccaagggg agcacgtttc
    15121 caccatgagc aaccgaaaac gccaagcctt cgtgcggcta ttgcgccggc agggcgggct
    15181 ggaagaggct cgtgtccgcc gattgcgggc tttgggggaa cttcggcggg ggcaatatag
    15241 aaaggcgacc cgggctatgg cttcccgggt cgccccaacc caactcacta tgaaacgcag
    15301 ggcggcggtt gtgtccgccg acccgctggg gcaggtccac cagtctgccc gtacgttttt
    15361 caaagcatga tttatgccag caattgggtt taatccaaac gccacccaga gtttcgggca
    15421 ccgggaatgc ggatgccgga aaaggctgaa acgatgagat gggattgagg gcgagtagta
    15481 aatgattgaa aaacagttaa ttgtgaactt gaggcgggtg gggagcggtt tttcgaggga
    15541 ggtgggccct tgagcacctt ctgatttcgc ggatggctta cttgaaacga ttcagttggt
    15601 cagtccagtc gtgcgccctc gttatttagg agcctgagta ggcagagcat cccggagcgc
    15661 agtctctccg aatcgtttag caggtggctt caccgttctg gccgttgaag ctcgagtgcc
    15721 tttgccggta aaatcgggag gccaggcgta ccgggaaatc aggagggagg gggaaccggg
    15781 attatcccgg ctaaggcttt acctgaaggg tggagaccac ttcgcgcacg ccgtcggttt
    15841 ccatggcgat cagaatggct ttgccgactt gctccgggga agagaccgcg cctgacaagg
    15901 tgacgacccc ggcggtggtg ttgaccgaaa ggttgaatgc gggcaggtcg cggctggtga
    15961 cgagcctggc tttgatggcg gcggtgatgc gggcgtcggc ggtggcatcg gcgattgcgt
    16021 ggccggcctg gcgcacttcg cggcggatga tctggccggt gcgcgccagc tcgtctttga
    16081 ggtcttcggg acgcaggcgg aagtcgcgga gtttgtcctg cgcggcgtcg cgggcgcttt
    16141 gggcggcttc ttcaaccttc tttccggcgg attgccagcg tggatcatgg cggccggtgg
    16201 tgaagaacca gaccgccgcc gcgccgaggg cgatgccaag ggccagggtc acgagggttt
    16261 tcatacgcag agggggctat tcgttgtggg ggattttgat ggtgctgccg ccgataaatt
    16321 cgcgaatgac ggcgtccccg ggaataatct cgcggctggc ggcctgttcg cgggtcaagc
    16381 ccgcgacgga ttcgagcagg tgcttgatgc gctcgtagcg gatgcgggcg tccaggaagc
    16441 cggtgtagga cgcgaaatct tcggggcgcg gcaggtagcc atccgggccg gcaaagaacg
    16501 gtttcagcgc cgggaggtcg aaggggaatc cgccgttgac gacatgatcg gccaggccgc
    16561 tcagggggat gatgctgaac agttcgccgg agcggacgta gtgccagtgc aggatggtca
    16621 gcagcgggct gtggttgcgg tgctggacat cgcgcagcat gcgccgcagc aggcgcacgt
    16681 cggtgaactg ggtgcggcgg ccattgcttc cgtcgccttc ggccagcacg ttttgcgttt
    16741 cgatgtagag gcggaattgc gcggcggcgg ggatgccttc cgtgatcggc gggtaaaggc
    16801 cgtggaggca atcgagcagg agaatctggc cgggttcaag cctgatgtgt ttgctcgcga
    16861 cgcggcggcc ttctttgaag ctgtagatgg gtttttcgat ggtttcgccg gccagcaggc
    16921 tggtgaggtg gccgttgagc aactggatat ccagcgcttc gggggtctcg tagttgcggt
    16981 cgttgatcca gtcggtcgga tgctcgacca gcgaccagaa atagtcatcg aggttgagca
    17041 tgaggaaccg cagtccccgt ttctggagcc gttcggtcag tttgacggtg gtggtggttt
    17101 tgccggagga gctggggccg ctgacccaca tcatgcggag ctggtcgccg ctggccaggc
    17161 gccgcatgac tttttcggcc gcttcgtcga gcgattgctc gtaaagggcg agggattcct
    17221 ccaccagggc cttgattttg ccgcgtcgga cgatttcgtt gaggccggcc acggtgtcgc
    17281 agccgcgctg ccgattccac gccagcgtct gctccatccg cgcccgcggg aagccgttgc
    17341 cggcgaactt gtccggcgtg atggcgcctt cccgcagcca gtgacggccc caccggtagc
    17401 attcgtaggc gtcggcggtg ttttggaagc cgtagctgcg cagcgcgtgg aggacttcgt
    17461 cctggatgat ttcgatggtg ggcgggaaat tggagatcag gtggtgcggg tcggagttca
    17521 ggcacacgac caccgcgtcg gccagaaacc cggcgatggc atcctcgctg cggtacgccg
    17581 caaagagggt ttcattgacg ccggggacgc gatcctgttc gaagccgccg atggatcggg
    17641 cggcgttgag gatcgcccgc cggatgcggg tgtcgtcgaa ctttaccagg gcgcggttgc
    17701 gcttttggac tttaacaatg cggttctgga cggccatgta ttttgactat acatcagccc
    17761 cggtggctga caatgccgtt cctgccccgg ttccggcttg acggcggggg gcgcgcgttt
    17821 ccccgcccgt gcgggcgacg gcagggagtt tccgcggcga tttctgattg tattctccgt
    17881 tactccggct attatccagc tatgagcggt gcctgttttc atcgtattgg cattggccgg
    17941 gggcagggct tgcgcgcacg ggtggcgggg gtcatggccg tttggctggc ggtggcggga
    18001 ggggcggggg aattccgcgg gctctgggtg gatagctgga gtccgggtct gtataacgcc
    18061 gcgcaaattt cgcaactggt aagcgatgcg cgggcaggcc atttcaatgc gctggcggtg
    18121 gaggtccggc ggcgggcgga cgcgttctac aactctaatt ttgagcccaa agcggttggg
    18181 gtggcggagg acttcgatcc cctggcggat ttgattgcca aggcgcacaa cacgaacgac
    18241 ggtccccggc tggaggttca ttgctggatc gtggcctacc cggcctggct ggggagcagt
    18301 ccgccgcccc agcccagcca tccgctgaat ctccatccgg attggcggaa ccggaccagc
    18361 acgggcgcgt tctgcaccga tgggcagaac cattacttcg atcccgcgca cccggaggtg
    18421 caaaagcata ttttcaaggt ggccatggat attgtttcgc gctacgacgt ggacgggttg
    18481 aactgggact atatccgtta tcccggggtc ctgtggggtt acggtgaggt cgccttggcg
    18541 cggttcaatt cgcgattcca ccgcacgggc tggcccgccg ccagcgaccc ggactggacg
    18601 gcgttccgcc gcgaccaggt caccgccctg gtgcgcaagg tgtatctttc cgccatcgcc
    18661 tgcaaacccc aggtggtgct ttcggccgcg gtgttcacgg gctcgccggg cataaccaac
    18721 acggcggatt ttccgagcac gagcgcctac agcagcgtgc tgcaaaattg gctggcgtgg
    18781 atgcaggagg gcatcctgga tttgaacatg cccatgacct attttgatca ctccgtctcg
    18841 gcggcggcat acgcgcggtg gaacaacttt atcaaggacc atcgctacca ccggcaggcc
    18901 gtggtggggc cggcgatcgg ccagaacacg atttccaaca gcctcgtcca gatccgcctg
    18961 acacgccaaa cgacgcccgc gggcaatcgc ggcgacgggg tatcctgtta tagctacgcc
    19021 agcaacaaca gtgggcggct gcccattgcg gcgtttctgg aagcgctcac gcagccgacg
    19081 gcccacgatc ccctcacgcc gccggtgttc agcgagctgg atgtgccgcc ggaccggcca
    19141 tggaaaaatt ctccggtgcg cgggcacttg aagggttttg tttatgacga tctgggccag
    19201 gaactggacg gcgccaccat cgcgctcagc gggccggcga tccgggtgct gacgaacgat
    19261 gccaccggct tttacggggc tgtggacctg gagccgggtc tgtacaccgc ctgcgcttcg
    19321 tttcccggcc atgccccggc aacgcagacg gtggcggtcg tggcgggaat ggtggcggac
    19381 ctggattttt cgctgacacc gacgggggcg gtgattgtcc tccagccgca ggatcaatcc
    19441 gcgctggtgg ggagcaatgc ggtgctgcgc gcgcgcgcgg cgggggtccg gccgctgggt
    19501 tatcaatggc tgcagaacgg ggtcgcgctc gccggggaaa cggccgccag cctgaccctg
    19561 gcccatattc agctcacgaa tgcgggggcg taccgtctgg tggtgtcaaa cgcctcgggg
    19621 gcggtcacct cgctggtcgc cacgctgacc acgctgctcc cggtcagggt ttcctccgcg
    19681 agaaacggca ccgtgagcct ggctcccaac gcggcggggc acgcgctgaa cagcttcgtg
    19741 acggtggccg gcgtcccgga ggcgggcttt ggcttcagcc attggtcggg cgaccttgcc
    19801 agcaccttga acccgcttac ttttcaagtg acgaacagcc tgacgatcac gggcaatttc
    19861 gccccttccc cggacgacct gatcctggac aatccgcaag cctcatacac gggcagttgg
    19921 acggtgagcc aaacggcgga ggggcggtat gggctgaatt accactacgc cacaacgacg
    19981 gatggcccgg ccacagcgac cgcgacgtac cggccgctca tcagccgggc cggagcctac
    20041 gacgtctatc tctggtaccc ggcgggcggc aaccgcgcga ccaacgcgcc ctgggtggtt
    20101 tcgtatgcgg gaggatcggt gacctcgctg gtgaaccaaa cgaccgacgg cgggaagtgg
    20161 gtccagattg ccgggggaaa atactttgcg gcgggaacga acggcagcgt gcaactggca
    20221 aacaataccg gcgcccgcgg caaggtggtg atggcggatg cggttcggtt cgtccgggcg
    20281 cgcgggcaac aatatgttcc aatccgcctt gagccgggca gccgggatgt ggcatccggt
    20341 ttcgcgctgg cggtgtatgg caacccggat caaacctgct tggtcgaagc cagcgcgaac
    20401 ctccggcagt gggcgccttt atcccgcgtg cggttgggca gccaactcgc gcaactggtg
    20461 gacgcggatt ccggcgctta cccgcaccgc ttttaccgcg cgcgccaggt caacgtgtac
    20521 tctctggcgg atttcgagaa ctatgccaac ggcgtccggg tgttgttcgg caccccggcg
    20581 tcctccggca gcacccatga atttgtggat tcggccgccc cgaatttctg ccgggtgacc
    20641 aactcttttc ccgcgggcaa tgcgagtgcg aaggtcttgc atatcggctg gagttttgtg
    20701 gccgggagca gcggccaaag cccctggctg cggctggcga catgggacgc cccgggcctg
    20761 ccgaatccga tcgtgagctt ccagcagggc atcggctttg ccgttcgcac cgaccgcgaa
    20821 atttacctgg ctgtgggctt gcgcgagacg tcttcgacgg gccccattgg gtcgaatggg
    20881 ggaacggtgg ggaccttgga atggatcggc ggcacttcgg ataatacgac tgacccgccc
    20941 cggggccgcc ggatctgcgc cggccaatgg atatgggtga gtttttgcat ccccgccgaa
    21001 ccggtgcgcg cctttgtcgg cagcggcaac ggcgtgctcg aatccagcac gggcaaaggc
    21061 gtcttcgatg agttggtcat tgtcccggcc gatggtctcg gcccctacaa tctttacctc
    21121 gacaatttcc agttcatcac cttcgagccc tgacccgcgc gggacggcgg gccggtttgt
    21181 gcggcatgaa atcacgccct ttcagggctt ggcgaggtgt gggattcttt cccagggcgt
    21241 tgccctgggc tatcacatgg cgccccgttg gggcgggggc gcaagacgga acgggtgcgc
    21301 caacgagacc ggagttggcc gcaacaggca agaggagata gcccagggca acgccctggg
    21361 aatagcgtcc cccaatcaac ccagcccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnagg agatagccca gggcaacgcc ctgggaatag cgtcccccaa tcaacccagc
    21541 cctgaaaggg cgaaacatgg atcaatccca cacataacgc cccgttgggg cggggtggcg
    21601 gccccgtttg ttgggacgtt gtcctggcta ttgcataacg ctccgttggg ggcgggcagc
    21661 ttgggcatct tccacggcgg gcttgaaggt gacgacgagc acgcgcttgg ccccgagctt
    21721 tttggcgaaa tggtgtagtt cttgatgttg gcggtcttga gttgctcggc acgcgctgct
    21781 tcacgccgcg cggagtctgc cctatcttgg gcaatcccgc atgcgccggg tcgccaatcg
    21841 cgtaagcata aatacacggg cgagtttccg gtttcggggc aaggatttct tcgaggatct
    21901 tacgcatcgc tctctcccac cgggcgaata gtggactcaa tgaactcgat ttcgtcggga
    21961 gtcagccgat atcttttgta gagtgcctta tcgggccacg actttgagaa gtcttgggcc
    22021 gggacaaaaa cgaatgaccc gcgagtgatg ttttgggtaa gcagaatggc agaaacaagg
    22081 aatcgcacaa atcgcgtgcg cagatattca gcaacgtgct ccgcctcccg ttctgatttg
    22141 aacggcccta taattaaata tgactccgtc gcaactgaat ttggggacat gacctcaatt
    22201 cttgacagca cgcgtttccg gcccgactta tcaggctggc ctgcatgctc gctcgatgtt
    22261 ttcgagagta gaactttcca tttcccgatg agttctgtcc ccttaataac atctttccga
    22321 tcaatccgac agtcgccact gctggtatat aaatatagct cgcccgtttt atttcccgct
    22381 tgggccgcag cgattccgaa tggccttctt gcagagacga gggttgaaaa ctctcgctcg
    22441 cctcgtgctt tcaccttctt cacgatgccg cgcacacgat tatcccggac gaaaacctca
    22501 tattcgttca gggatctctc cgctataaca ctttggccat tggcgcgagt ctccaccatg
    22561 caagtgccgg ggttgtcccg atcccaaagg aaatacataa cgccaccggc aacatccacc
    22621 ccactgaatg cgtctcttga gttcggataa tcaattaaat gcctgatgcg ctggtccgag
    22681 agcatcttag cgcgaaactc gtccaatcct ttgcctcctg agaaccattt cgatggcacg
    22741 atcattgaca aaaatcttgg ttcgagcgag agcgcttgct cgatgaaacg atgatagagc
    22801 ggtatggcgc ttgagccgtc gcctccaccg tcattaagct gatacggcgg gtttccaatg
    22861 atgacgtcga attgcatgtt gcctccaaaa atctcggcga gccgagtctt gatgttgctg
    22921 tgggaatgaa tgcgcaggcg tgagtttcaa gtcccgccgc ccggtcgaaa acggatttgc
    22981 ccgcgccgca atagaggcac tggttgcctt ctcaagagtg tttgatgcac tggaaccaga
    23041 tgttcccatc gtcgttggag aggttggtga tgcaggtcag catgtcgggg ttgcacccgc
    23101 ggagtgcaaa gttggtttgg gcaatcatta ggcggcctcc agcatggcgc cccacacccg
    23161 aatgcggcaa gacgaaatgg gtgcgccaat ggcccggttt ggccgcaacg aggcaaggcg
    23221 gaacgggtac ggcaacgggc ccccgcgccc caacggggcg tcatgcgata gcctaggcaa
    23281 cgccctggga acagcgcccc ccaaccaatc cagccctgaa agggcgaaac acgaaccaat
    23341 cccacacata acgccccgtt ggggcggggg ggggggggcg gccccgtttg ttggggcgtt
    23401 ggcccggggt ccgcattatg cctcgttgaa aaccaggggc gcgaggattc aagcaaggaa
    23461 ccgcatcttg aaatctcgcc ctttcagggc ttggcgacgt tgcggggctc tttcccgggg
    23521 cgttgccccg ggctatcacc gggcgccccg ttggggcgag agtgattcgg cgattattat
    23581 gcgcccccgg tgtggtggca tgcgcccgaa tgcggcaaga cgaaatgggt gcgccaatgg
    23641 cccccccgcc ccaacggggc gtcacgcgat agcccagggc aacgccctgg gaacaacgcc
    23701 cccaaaccaa tccagccctg aaagggcgaa acatgggtca atcccacaca taacgttcgt
    23761 catacgcaac gttgtatttt ttcagccaac gccggtattc ctcctgaaac gtcatcgcgc
    23821 ggtgatgctc ggcttgctcg ccaatgtatt tcgcatgcgc cgcgcggtgg gatggactga
    23881 gcgagaaaat gccatagcca ctttgccaat gaaagcgggc atagcgaagc cccttcgttt
    23941 tcagccattt ggatgtgccg gtcttgatct cctggaccaa gtcggccggc gcgcacgtac
    24001 ggggcatggc gatcagcaga tgaatatggt cggcgacgga gcccgccttg agcagtgtgc
    24061 cctgttggtt cgccacgatg cccccacagt acgcgtggag ttcatcccgg atgtcgtcgg
    24121 ccagcaccgc ctcccggttc ttcgtggaga agaccaggtg cgcgaggatt tgggcaaggg
    24181 attgaggcat gaaatcacgc cctttcaggg cttggcgagg ttgtggggct ctttcccagg
    24241 gcgttgccct gggctatccc atggcgcccc gttggggcga gggtggcccc gtttgttggg
    24301 gattgccccg tttattgagg gtggcccagt ttgtcggagg ttgctccgga ctatcgcatg
    24361 acacctcgtt ggggcgcggg gggccgggcg tgattggggg gctttttccc ggacattgcc
    24421 ctgggatatc gcaatatgcg cccgggcagg agttggcgga tttatagtgt tgcctgcggc
    24481 gtcctggcga tcagctcact cacagtcatc ggggggtagg ttttggtggg cgtgaaaatc
    24541 tcgtgcttgc cgagttgggc aaagagcgag tcctgagcac tgaacgccga ggacaaagtc
    24601 aggctttgca ggctaaagtc gcgccgctgg taacgtcctt tccaaagata agcccactcg
    24661 gcgaaggtga tgggcaaatt gtcccaggtg cgcattttca tggcgtcgcc gtggatgagg
    24721 ttgaccgaca gcacgcagac ggcggcgcga tagaaatcgt cggaaggctc gaggttcaaa
    24781 tactcggcga agatttcccg caggttggcg cgacaatcgg caatgttgtc cggcaggatt
    24841 tcaatgccgt aaacgcacat cagcgcgagc agtgcgtagt gccgccgctc gaaatcggat
    24901 ttgccgtatt tgagttccac agcggcgagt ttgcgcctga ggatctggac aaggaagttg
    24961 ccgttgccgc acgccggttc cagaaagcgc gagtcaatgc gctcggtctc gcccttcacg
    25021 aggtcgagca tagcctcgac catccacgcg ggggtgaaaa cctccccgtg gtcggcgacg
    25081 cgttgtttgg acttcaccag cgccatttta cttttccaga gtcgaaccag gtcaaagccg
    25141 ggctagagcg cagcagcctg gcttccgcga acaaacggca gactgcagct tcataatgcg
    25201 gcagaaggcg gctttttcgc aacgaaataa cgatgccgta tttctccccg caccggcggg
    25261 catgctccgg ctccggggcg gccatttcgc cgcggctcca aatcccgtcc gctctcccaa
    25321 gccaatcgcc tggagctgag gttggaggaa agcccggggc cacggttgcc gcaacggttc
    25381 agggggctga aaatggtcgg ggcggtgaga ttcgaactca cgacctcttg tacccgaaac
    25441 aagcgcgcta gccaggctac gctacgcccc gatccaatgt cgcccgaatt taaccctttc
    25501 tgccgccgtt atgcaactct gaaaatgcgg cggcggcgga gcgcgttgcc ggcggctggc
    25561 tccggttggc cgggcgccat gttcggcgct tacttttccc gggctgccgg ggcggcgacg
    25621 gcttcgtcca cggcttcgct gagcaccgcc agcccttcgc gcagggcatc ctcgcggatc
    25681 gtcaggggag gggctatttt cacggtttgc ccccaggcgc ccaccggcgc gaagaacagc
    25741 agccctttat ggaaacagcg ctcgatgatg tcgtgcgcca ggtcgtggtt cggctccttg
    25801 cgccccggct gggtgatttg caatccgccg accaggccgc ggcaactgta atggccgatg
    25861 acctgcgcgt gccgctgctg gatggcccgc aggcccgcgg cgaggatttc gcccaggcgc
    25921 gcggcgttgg cggtgaggtt ttcggccacg atctttctca cgctggccag cgccgcggcg
    25981 cagcataccg ggttgccggt gtgggtgctg gtcatcgagc ccggcccgaa ttgatccata
    26041 atctcactcc ggccaatgac cgccgagagc ggcagcgaac tgctgatgcc tttgccgcag
    26101 cagatcaggt ccggcgtgac gccgtaatgc tcgaaggccc agaaccggcc cgtgcggcca
    26161 aagccggcct ggacttcatc gaagatcagc agggcctcat gcgcccggca ccattgggca
    26221 agctggcgaa tgtaatccac cggcgcgaag tccggcccca cgccctggta ggtttcaaag
    26281 atcacgccgg cgacattctc cggcaccagt cctttccggg ccagcgttgc ctggaaaagc
    26341 tcgaagcggg tgtcctcggt ccagtagccg tccgggaagg gaacctgcac gatcgccggg
    26401 tcctcgttga cgatccagct cttttgcccg ggaatgccgc cggcttgctg cgcgcccagc
    26461 gttcgcccgt ggaagccgcg ttccgcgccc acgaggccga tcttccgggg gccgcctttg
    26521 cggatgccat gggcgcgggc cagtttgagg gcgcactccg tcgcttccga accggttgtc
    26581 agcaaaaaga ctttctccag ccccggcggg gcgacctgga tcaactgttc cacgagcgcg
    26641 gcgcggactt cgctgggaaa cacgtagtta tgcagcaggc cgtggttgac ctgggcgaga
    26701 atcgcctggc gcacctcggg cgcgcaatgc cccgcgttgg tcacgagcac gccgctgctc
    26761 cagtccagcc agcggttgcc gtaggcgtcg taaacgaaca cgtcttccgc ccggtcccag
    26821 accagcggcg gctgcccgcg catggattgc ggctcgaact ggcgcagctt ctccagcgtg
    26881 ggcaccgaat ccgggtgcgg caggggcgtg acgatgcggc gatgccgggt ttcgacccgc
    26941 gcaacgtggc gcggcgtgat gctaaactct ttgcccataa ttgatgcgcc ccatacaacc
    27001 cgcattgacg ccaattggaa agccggaagt gcgcggaaga aaccccgcgc tgcccccggg
    27061 cgccggcgaa agaccgcccc cgcgggccgg cgcctgaacg tcccgctcct cccggcgcgg
    27121 gattccggag agcgttcact ctgtgagatg cccgccaaaa gtaatttcag tcggcgggca
    27181 atcgtattag gatggccggg atgaagaccg cgcaacagcg aatcaaactg gcccgaatgg
    27241 atccggaaca ggtggcggat gagatcgggg gctttatcgt ggaagccgtc cgccggctgc
    27301 gcaagaccgg cgcggtgctg gggctgtccg gcggggtgga tagcacctgc gtggcggccc
    27361 tggcgaaacg ggcctttgat cggcataacg cgcagtcccc cgatcagccg ctggagctgg
    27421 ccggttacat tttgccctcg cgcaccagtg aggccaggga cgtggaggac gcccgccagg
    27481 tcgcgacccg gctggggctg cggcatgaag tccagagcat cgagcccatc gtggaagcct
    27541 tccagaccac caaccccgaa gcgctgcgcc atgcgtatca tcgcggcaac ctgacttcgg
    27601 agattcgggc ggtgattctg catgccaagg cggccacgga aaacaagagc gtgattggca
    27661 ccggcaaccg ggatgaggac tacggagttg gctattatac gttattcggc gacggagcgg
    27721 tgcacctgag cccgatcggc aacctgccca agcgcctggt ccgggagatg gcggcgcatc
    27781 tcgggttcca ggacctggcg ggccgggttt cctcggccgg cctggagccg gggcagactt
    27841 cgtttaagga tctgggctgt gattacgaat ttgccgaggt gattctaaac ggcctggacc
    27901 agggcctgcg ggcggaggag ctttccgcgc atccccaggt cgtgccctat gcccggcagc
    27961 aaatcgagaa atacacgcag tggtatggag cgcccaaatt tgcgtccgtt ccggaactgg
    28021 tggccgaggt gttgcggcgg catgagctcg cccggctgaa agtccggctg gtccagcccg
    28081 acatcgcccc gatcaccctg accctggagt gagggcgtcc cgcacgggcg cggttccccc
    28141 ggaggacatc cgtcagtccg aggagatcag cttttcccag agcgtctcgt tcagcagcac
    28201 cttttgcagg aagctttctt cggtttgcgg ggatgcggcc cggttcggat cgagcggcaa
    28261 ccgcagccgg aacgtggttc cgcgcccggg ctcgctccgg gcgtcaatcc atcccccgtg
    28321 atggtgcacg atgaaatagc aagccatcag gtggatgcca tattcggccg gactgtcgct
    28381 gcgcagcaca aacgggtcga acaccagccg cagcgcctcc tggggcagcc ccggtccgtc
    28441 gtcgttcagc tcgaccagca cctcgggctg gcccggcggc ccggcatcca gcagcttggc
    28501 ggtcagggtg acccggttgc cggcggacag gctgacgagc tcatcccgga gcagcagatc
    28561 aaacagccgc gtgaacttcg gcaggtccac gttgagcgcc ggcagggagt cggggatgtg
    28621 gttctccacc acgatctgct tgccggcaaa cgcatccttc aagctggcga tgacgcccgc
    28681 caccacttca tgcagatgcg cgcggttggc aaactcatag gtgggctttt ccgaagcggt
    28741 ccacagatcc ttgagcaggt ggctgatctt ttcgatttgc ccctggacgt tctggtaata
    28801 ctccttccag aaatccgggt tgcgcaaact ctccatgtcc agcttctcct cggccatctt
    28861 ggagggcgcc aggtccagga acgtcttgac cgccaccagc gcgttgcgga tgtggtggct
    28921 caggccggcg gccagcaggc caaggctcac aatccggtcg gcaatcatca tattgtgcag
    28981 cacggacatt ttctccttca gcaactggtc gcgctcgcgc tgcaccatga aaaactccag
    29041 cccgcgctgc agggtgtttt ccagttgcgg cacatcccag ggtttggtca cgtatttgta
    29101 aatggcgcca gtgttgacgg cggcaatggc cacctccata tcgctgtagg cggtcgccag
    29161 aatgcgaatg atgcgcgggc gaagctggcg cgccttttcc agcagccaga tgcccttctc
    29221 gccgggcatc cgctgatcgg tcatcagcag cccgatttcg tctttgtgcg cctccagcag
    29281 cttcagcccc tcttgcgcat taggcgcggt aaagatgcgg aactgatccc ggaacgcccg
    29341 ggtaaaatac ttcagggaca gctcttcgtc gtcaacgtag aggatcgcaa atctctggta
    29401 gtcgtaaaga ttctgcatag ccgttgtctg atcgtttcat ttatggcgtt cccggggcgg
    29461 gaaactccag cgtaatctca caaaactggc cgggctccga ccgcgccgaa atccggccgc
    29521 cgcaattctg catgatcgtg tggcacgtcg ccaggcccag ccccatgccc tggccgacat
    29581 ccttggtggt gaaaaacggg tcgaagatct tgtccaggtg ctccgccccg atgccggggc
    29641 cgttgtcccg cacccgcagg atgctcttgc ccggttcgag gcatccctcg atttgaatgg
    29701 ccgggggctc gcccgcgaag gtcttggtct tgagcgcatc cagcgaattg gtcagcaggt
    29761 tgagcagcgc ctgcgtcagc aggttcttgt tggcccagac cgtttgccgc tccgccagct
    29821 tcgactcgat ccgcaccttg cctttccatt cgttgctcag gaaccgcagc gccgcggtga
    29881 ccacttcggc cacctccacc gcgtcccggc tttcggtctc gggatgggtg aacgtgcgca
    29941 agctggatac aatatccttc acccgctgga ccccatcctc cacgtccttc agcacatccg
    30001 cgtattcctc ctgctccgcg ggcggcaggt gccggctctt cttgcgcagc gtgaacagcc
    30061 ccgtcgcggt gaagttcagc ggattgttaa tctcgtgaat gatgcccgcg ctcaggcggc
    30121 cgagcgacgc cagcttctcg gattgcacca gttgcagctc ggtttctttg agctgctgga
    30181 tcgtggcttc cagaatccgg ttctgccggt ccaactggta gcgaaaggca aattccccaa
    30241 agcggatctt gctgtgaaaa tagctgcccg tggccacgat gattcccgtc agccccagga
    30301 aatagacgtt gttgacaaaa agatgccagg ccacgggacc ctgattaaag aagaccgcgg
    30361 ccaggtagag gcccagcacc agcacggcgg cgcacaggct ctcgcgaaac gtccaatgca
    30421 gcacaaacgc cagcaccagc agcaccaggt tcaggccggc gtagtagggc gaggccgcgc
    30481 catccgggct gaccttgatc atgtaggcca tgcaggaggt gggcagcatg aacagcgtga
    30541 tgcccaggaa ccggtaatac ttgtgcccca gcggcgtgag cagcagggcc aggaaggcgc
    30601 cgatcaacac cgcgttctcc agccgcaatt tgaggaattg cgccgccagc gcgggataaa
    30661 ccgcgtggtc aataatgacc ccggccggca tcagcaccat gccaatcacg caggccacct
    30721 tgaagttgct gatcgtctgc cggcgatcat actccgcaaa cgccgcctcg atctcgcggt
    30781 tatggggcgc gccgtcagcc atgctccggt ttcctcgctt ccacaaaaac attcacctcc
    30841 gtcgggtcct tgaggacgcg cgtccgggcg gcggaaagcc ccggcggcag cagcgccgcg
    30901 ctctgcgcgg ggttgcggta gaacaggtgc cagtccagca caaattccag catgtggcgg
    30961 aaaggcttgc aatcctccac gttggtggcc gccatccgcc cgcccggcgc cagccaatcg
    31021 taaagccccg ccatcacctg cgcggcgatc cggtccgaca ggtaatccag caggccggcg
    31081 caataaatga aatcatattc ccgaaccggc gccgccgccg cccgggcgcc ctctttaatc
    31141 acctgctgca ccgattgccg ccggatgtcc agctccacgc gccgcccatg gcgccgccgg
    31201 caatcctcca gcacccgctg aacgtgccgc gtcgtttcct cgttgaaatc ccacagctcg
    31261 aagcgcgccg ggtggcccaa ggcgtcctcg gccaggaact cctggatttc ccgcgccggg
    31321 ccgcacccca ggctcaacac gcgcagcggc tgcccccggg tcgcccggcg gcgcgcctcc
    31381 tcccgcagca gccccttcaa atactgaatg cgattgcggt gcgcccgcgc cggccattgg
    31441 ctcaggaacc accggtttac caccttcgca aacagcgagc cgccctcatg cgggtcgcgc
    31501 aaaatcatgt tcaccatctc gtagtcgccc gcgtagccca gcggcttgtg gtaggtgcga
    31561 tgcgcaaacg gcgcgcacag gaccagcgga tgcaacaggc gctgcgcgaa aacctggtgc
    31621 accggccgca gctccggctc gaggtccgcc gcgatgctct ccaggcgctc atgcatcgcg
    31681 tcgaacgccg gcaccatgtc ccgtccgatt tcgcgcgcgg cggcttcctc ccgctccgcc
    31741 cgcgcgccgg gccgggcggc gcgaatctcc acctccgtct gctccagcca gagccgcagg
    31801 tcgctcagaa aggtctgcat gtcggccacc accaccttga aggcgggccg cacgcggtag
    31861 agcttctgcc aatggcccag gaacgcctga aacccctcgt gcaagggcgc gggatcctcg
    31921 cccggggaaa aggaagacaa tcgaaagctc ccctcatcca gccggacttc gccgatcagc
    31981 atcgtgcccg cgttgatcag cgcggtgacc accgcccgcc cctcataaat tacccgccct
    32041 tgaagaatga tctggaagcc ctcgagcacc tccgaaagct gcagcaccag gccgggctgg
    32101 taaatctcca gcaccgccga ataccgggta agccgccgca accgggcgcg cagctccccc
    32161 gcctggcggc ttgcgccaag aaccaggttc tctgtggacg cgccgttttt catgatccgc
    32221 gggaccgcgg ccgtgagcgt gtcgcaagcg ggcgggcgaa gcaacccctt agcggccgcc
    32281 ccgctcgcgc tcgactccgt ccgtggtctt ttcatgcaat cttcacgcgc cggcggacgt
    32341 ttgcaacccg cgcttcccgc cgcgccccgc ctcaggccgg agggccgccg cggccgccgc
    32401 ccgccgcccg ccacccgcgc agcagctttc gcagcattcc ccagcagccg tccgcaatcc
    32461 ccgccgaggc aaacgccagg tacacatcgg cgcacggcag ccagccgttg gacgcaaagt
    32521 aaagcggccg ctcggcgccg cggtatttcg atttgtgaaa cgacaggccc tgaaaattat
    32581 acaggccatt gccaaaacgg gcgctccacc ggaacaactg ccgggccgcc cagtcgtcgt
    32641 tgaacgcccc gcactccagc ttcccaaagg gcgccagcag caaattgagc acctccaccc
    32701 cctccgcccg gaatgtttcc atggctacca tgtgcagggc cgtggccagc cgcccaaacc
    32761 gccgctcgtc gcagcgcacg atgttcgccg aatagccaaa cacccgtccg ccgcgataca
    32821 tcggatcata aaacgcaaac cccgccaccc ggcgctccgg gtcgtaggcg acaaacttgc
    32881 gcacgccttc ctccggctcg aaaaccgggc ggcgcgcata aagccaaatc tcccggtcat
    32941 tgatcttccg ggagcgaacc caacgggccg agaaggcttt caattcccgc cgccgctgct
    33001 tcaggctcat cccgctttcc tcgcggatgg tcagcccctc acgccgcgcc tcattgcggg
    33061 cgcgcttgat caagtccaat tccttccagt tgccttccgt gttgtaggtc tgaaccgcca
    33121 tgagcggctc atacccgatg caattcactt tgaagcccaa cggccgcagc gcccgggcgc
    33181 acgcctcgga aatgacgccg aaggccgcgt gccggtcttg cgccaaaaag ctgcccacca
    33241 gggccggaac gtcctccggc gcgcagatcg ggtcggaaaa aacaatgcgc cggggccggc
    33301 gcgcccagag cgggtgccgg gctcgcgtat aggcaatata accactcggg ccgataaaat
    33361 actccatccc cgactgcagc gtcgcgtagg cgatgccctc ccgcccatgc tgccgcagaa
    33421 acggcgaaag caaatcccat ttctgccggg aatccaacgc ccgggcgctt tctccttcca
    33481 gaacgcgtat gccggcgcct tgcgccgggc tggcagcctc cgcccctcgc accccggcct
    33541 tcggcgcccg ccggatccag ccatccgccc gctcccgcct cgctacgggg ataaaactgg
    33601 ccggctgggc atcggcgccg gcaagcaatg ccgcatccga atccaaaact gtgccctgac
    33661 cggagtggtg catggaccac ggcagcgcag aactggacgg ccaattcatc tcttaagccc
    33721 caattacagc gttcccggtg ccaaccgtgc gccacaggca ggaaaaatgc aaaaatctcg
    33781 ccttttcagg gactttacgc gcgaagggca ccgcaaatcg cccaatttca ccggaaaccc
    33841 ctgaaccggc gcaaatcgtc cagaacgcat ttgtgacatg gccaccctgg acaatcccat
    33901 ccccctccgg gtttaacccc ctccgcgggc gcgccaggcc gctttttcgc ccattctaca
    33961 agcgctggcg accccacggc atggccctcg ccagttctaa gccgtcacga ttcagttggg
    34021 caactcaact gccagccccc attccatcag aatcaggaca gccctgatcg ccccggccac
    34081 agccttggcg aggtagtgtc ctaaaagttc tgcaaaaagc agcgggtcat ggtaagccac
    34141 tggg
//
LOCUS       DAOPQG010000028        33817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1700_length_33817_cov_14.889906, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000028 DAOPQG010000000
VERSION     DAOPQG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 33817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33817
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1700_length_33817_cov_14.889906"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKI07_04800"
     CDS             complement(<1..679)
                     /locus_tag="PKI07_04800"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOC50403.1"
                     /translation="MVPHCDSPGFLRRRLTREVIVGDPQRGGVIIGGRHPVVVQSMLT
                     CDTMDTAECVRQTLDLVAVGCQIVRITAPTVKDAANLENIVAALRARGCLVPIVADIH
                     FKPEAALEAARWVEMVRINPGNYADSKKFIVKEYTEAQYAAELARIREKFTPLVELCK
                     KLGRAMRIGTNHGSLSDRIMNRFGDSPLGMVESALEFARIARQHDFHNFKFSMKASNP
                     KIMIQCYR"
     gene            complement(682..2067)
                     /gene="rseP"
                     /locus_tag="PKI07_04805"
     CDS             complement(682..2067)
                     /gene="rseP"
                     /locus_tag="PKI07_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOC50404.1"
                     /translation="MWLLNIVYVVGAVILLFGAAVFVHEYGHYWMARRRGLKIEGFSI
                     GVGPKIVSWMRDGVEWSWRWIPAGGFVKLPQMATSDALEGKADENSPPLPPASPWSKI
                     LVAFFGPLMNVLFGIAIACLLWVVGIPVQVNPPIIGYVDPDSREARLGIQPGDQIVSV
                     EGKPISSWQEVQEATILARSTNLTVVIARVGQFTNTYELNTVVSEALGLKVLNLDPRD
                     HPVIRRVLSGSAAEAAGLKAGDEILSFAQVPLHGADQFIKLVQKRPGESHDIIIRRAG
                     ARQTLQVTPRLDPGVKVGRIGAEIGTGKPVYRIEHISPWTQIASVLERTISTFSALFH
                     SKQTGVGLKDLSGPPGILAILAAYVNSDWRLALSFLVLLNINLAIINLFPMPVLDGGH
                     IVMACVERLFGRPLPGRLVEYTNTAFAMLLIGFMLYVSFNDVRRFSLFRSMFKQEVTI
                     EKPESAPDGGR"
     gene            complement(2166..3305)
                     /locus_tag="PKI07_04810"
     CDS             complement(2166..3305)
                     /locus_tag="PKI07_04810"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOC50405.1"
                     /translation="MRNVVLLGCTGSIGTSTIKVAEDLRDCIRLVGLAAGNNAELLLA
                     QARKLQPAAISIADPSRAQELRDLLGGGMAVFSGAQGLVKLATLPEADIVLIAIVGTA
                     GLQPALAAIRAGKDIAVASKEILVMAGEIVMREAQAHGVRVLTVDSEHSAIFQCLEGK
                     PAESVRRLWLTASGGPFRTTPREAFPDITIERALQHPSWVMGRKITIDSATLFNKGLE
                     MIEARWLFDVEMARVAVIVHPQSVVHSMVEFVDGSILAQLSTPDMCLPIQYALTYPAR
                     APSNRVQTNLAKLGQLTFEEPDPERFPALTLARRAGEIGGTLPAVLNAANEVAVEAFA
                     SRRIRFPDISAVVRRVMDRHPVIPHPTLEQIIEADQWARHEAERG"
     gene            complement(3323..4258)
                     /locus_tag="PKI07_04815"
     CDS             complement(3323..4258)
                     /locus_tag="PKI07_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HOC50406.1"
                     /translation="MLDKTAPTPPATAVALSKSQVFLRRLGSFIALWAVVLTALFSSN
                     RSVSDYVFLLAMVLLAGLGLLEFYGLAAKRGLGCFKYWGVTGGVLLMVGTFLNLTGLL
                     GTSGAPARVNDFEASFLIVFVLGLCLCQLFSRRHPAGLLTIALTLLGLMYVAWLLNFV
                     QKINFFFYPGTAGCYYLLFFIVITKFSDTGAYAVGSLIGRHKLCPRISPAKTWEGFVG
                     AIAISTLASLLLAHFLGQKLPGMNWVHALILGVVLGMGAVVGDLIESLFKREAGAKDS
                     GSFFPGIGGILDLLDSLLFNAPIMYLYLRHVLSER"
     gene            4728..5792
                     /locus_tag="PKI07_04820"
     CDS             4728..5792
                     /locus_tag="PKI07_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOC50407.1"
                     /translation="MLLAADAGPWPVSKANDWLAKQGWLVGCNFSPSSAINQLEMWQA
                     DTFDPATIDRELGWAEQLGFNSIRVFLHHIPWEQDRAGFLKRLDEFLNLAAKHHIGVM
                     FVLLDSCWHPFPKPGKQPAPKPFVHNSSWVQCPGLDILKDPARHDELRGYVTGIVRHF
                     ATDRRVQVWDIFNEPENRNHISYGQYEPENKAELALALIKKAYAWAREAKPSQPITAG
                     VWMGNWADPAKLSPMEKFCLEQSDIINFHSYGSLPVIQKCVQNLRRYHRPIVCTEYMA
                     RPQGSTFDPILGYLKEQKIAAYNWGFVAGKTQTIFPWDSWEKQYTNEPPLWFHDIFRR
                     NGQPYRPPEVEYIKRVTGKP"
     gene            6201..7043
                     /locus_tag="PKI07_04825"
     CDS             6201..7043
                     /locus_tag="PKI07_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOC50408.1"
                     /translation="MRRVVFREMVLQRPRFSGLAPRVRRVLLALFLLQFALVWARVWW
                     PGLLFGHARWPDGLLLVLANAVLLAALTCQLPGQNVMLAALIIAVLAGAVQCLNALTA
                     IPFGPCVYTGNIGRELFPPLPWAVPLIWLAAILASRGTARLILRPWRKTRNYGFQMIG
                     VTTLLTVLLDAGLEPFATRVAHYWFWNPVKLKFGWYTAPGVNFLAWAATTLLILAFAT
                     PALINKQPARGSAPDYSPLLVWLLFMLLFATGAAGHQLWPAFGLTCAISITVAGVALR
                     GARW"
     gene            complement(7054..7854)
                     /locus_tag="PKI07_04830"
     CDS             complement(7054..7854)
                     /locus_tag="PKI07_04830"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOC50409.1"
                     /translation="MDRRLNRDYRARLAIIRVALGLLLCAVGAPMAAGAAETIYIVKR
                     GDSVYSIGRRYGVSPAVLAERNGLNRNFYISAGQRLRIPTRRVAPAKKPAAKPPPPSV
                     LPRAVREAIDKAPVRAGRWKSIVIHHSGTDTGTARTMDRYHREVRRMENGLAYHFVIG
                     NGNGMGDGAIAIGQRWTRQLDGGHLASAAQNKTAIGICLVGNFDKRPPSPKAMTSLRL
                     LVEALMTRCRLTPTAVKTHQQVNVRHTRCPGARFPTQSFLKSLPTPKR"
     gene            complement(8085..9431)
                     /locus_tag="PKI07_04835"
     CDS             complement(8085..9431)
                     /locus_tag="PKI07_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOC50410.1"
                     /translation="MNGEALLSIALHVLGVIVLVLLNGFFVAAEFGLVKVRDTQLSPL
                     ARDGQRRAQVAVFILQRLDSFLSAAQLGITLTSLGLGWIGAPVFAVLLKPVFDLAGVE
                     SPELREWVAFLVGFSIITFLHISAGEQAPKWLAIQKPLATTLWVSYPLLWFYRASYPF
                     VVLLNDSAQWMLRQVGLGPTSEGGRTHSEEELRLLLWAAQKQAGGSALGRDIVLNALD
                     LRRRVAREVMRPRHEIVALDTEASIAECLDVAEKTHYSRLPLCEGGNLDRTLGVVHIK
                     DLYTMRTKARRGADLLPVARKLIYIPETARLEKLLQFFLERKLHLAIVVDEYGGTAGM
                     VTLENILEELVGQIQDEFDQEKPLLVPAGENTWEAAGTLPLHELEELVGEPLREEGIS
                     TVSGFLTRRLDGFPKVGDVLKLGACKLQVEEMAGMLVARVKVTRTTTGSPKAGLSG"
     gene            complement(9433..10422)
                     /locus_tag="PKI07_04840"
     CDS             complement(9433..10422)
                     /locus_tag="PKI07_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOC50411.1"
                     /translation="MTAHTLIWTIFGACLGLSFVLAGMEAGVFALSRLRLRRQMRAGK
                     PSAKVLHDYLENPENFLWTILVGSTLANFLILGWLVVQLHAALAGRRLWFFGVFVVAV
                     FLFYTFFDLLPKMLFRRYPNRLCVALARPFRLIHLALRPLVALVEAFSGALLRWRGGK
                     AFTGHLFGNREEVRLLMQESAQVLTSEERQMINRVLDLQALTVRQAMRPLEQAVTLAA
                     QTPASAALALCRERKFTRLPVWEMRGGEQRIIGMLAVNSLLYQPALDERRPVSDYMKP
                     PFFLAEDLRLEVALRRLQRSGQRLAIVLGRDRREIGILSLQDVLKLIFGEVSL"
     gene            complement(10437..11642)
                     /locus_tag="PKI07_04845"
     CDS             complement(10437..11642)
                     /locus_tag="PKI07_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOC50412.1"
                     /translation="MAVLIFAGASFFFALAETALFSLSKWQVRQLAGRPSRAGSAVAQ
                     LLAEPQDLLATMVLGNTFASAAMLAVALWEAIHGQWPLVATVAGLLVLNLIGCEVWPK
                     TLAVRRPEQWALRVARPMAFLLRFGLPLCRGAQKVNTAILGMVARRTGSPGAVVTDAD
                     YQELLEMAFQQGALAQSEKEIILQIINLDQRTAREVMKPRSRMAAISSDLSAEEMIAA
                     ARKYRHRRLPLYDEETDTIVGVLNTRALLLNPQIDLADAIELPSFVPELMNLLQLFRA
                     LQRQQRGLAIVLDEFGDTAGIVTMEDILEEVIGKIQGEAEAEGFVMERLEPGRWRVAG
                     TLRLDDFRREYPALADVPEVETMGGLLASLLDVVPARGDAATFSGLRLTAQAADERRV
                     RELLVEVMR"
     gene            complement(12449..13558)
                     /locus_tag="PKI07_04850"
     CDS             complement(12449..13558)
                     /locus_tag="PKI07_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOC50413.1"
                     /translation="MTDPRYRKLAKLLVEYSTALKRGDCVLLDMIDVPDAFAIELMRA
                     ARAAGATPFIEIRHTRVNREILRDIHPRHARLLRDFELFRMKKMQAYIALRGSDNASE
                     NADVPGHQMALYSRITRPVLDYRVKRTRWCVLRWPTPSMAQAAGMSTEAFEDFYFEVC
                     TLDYRRMGRAMTPLVRWMQRTDRVRLKGPGTDLAFSIKGMGAQMCKGDRNIPDGEVFS
                     CPVKDSINGRIQFNTPTLYSGTKFENVCLEFKAGRIVKATANNTRRLNEILDTDPGAR
                     YTGEFSLGLNPLIQKPMCDILFDEKIAGSLHLTPGQAYEECDNGNRSAVHWDMVLIQR
                     PDWGGGEIWFDDHLIRKNGLFVPKDLKPLNPANLK"
     gene            complement(13912..14574)
                     /locus_tag="PKI07_04855"
     CDS             complement(13912..14574)
                     /locus_tag="PKI07_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HOC50414.1"
                     /translation="MKRTPAINDLLAIMARLRSPRGCPWDRQQNHRTLRWHAVEEVYE
                     LLDAIEAGDDHELEEELGDVLLQVVFHCQLARERGAFDFERVARRIADKLIRRHPHVF
                     GRLRVKNVDQVWANWEQIKRAEKQGTRHARPSALDGIPRSLPALLQAAKLVRKARKAK
                     LLPPETTPPVRTRRELAAQLFALAQYAQERGWSAEDLLRRETRRRERALRRREQMPEA
                     RR"
     gene            complement(14672..15748)
                     /gene="proB"
                     /locus_tag="PKI07_04860"
     CDS             complement(14672..15748)
                     /gene="proB"
                     /locus_tag="PKI07_04860"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOC50415.1"
                     /translation="MRSEIIKDITRIVVKLGTGVLTDSRKQPELAQMEQLVGQLAEQR
                     QAGREVLVVSSGAVGAGMGALGFSQRPRALADLQACAAVGQSRLMAVYDELFGKHGLA
                     VAQILLTHDDLEHHERHLNARNTLVTLLEHGIIPIINENDVISFTELKFGDNDLLSAL
                     VASLLPADLLLILTTVDGVLENFGKANSRTIPLIRRVDAALEQTATGTRSATAVGGMA
                     SKVQAAKIVMRAGIPLVIASGRKKRVIARVMAGEEEGTLFVPQPTRLKGRKRWIAFFH
                     HPKGALYVDAGARKAVREGGKSLLPPGITRCDGDFAAGEVVRICDQDGTEFARGIAGF
                     GAAEIKARKLQRVEVVHRDNLVIL"
     gene            15893..17191
                     /gene="gltS"
                     /locus_tag="PKI07_04865"
     CDS             15893..17191
                     /gene="gltS"
                     /locus_tag="PKI07_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOC50416.1"
                     /translation="MSGSETLIVIPALFIAILAIPVLLAGEWLVKRFRLLARFNIPAP
                     VVGGLLVSALLLLGHLSGAFAARFQIHVTARWWTWLVTAEPEWFQAPGKHVNTPFLVA
                     FFACIGLNARWELVRRGGAQVLLFWGAAAALAVAQNGIGVALAKLIGAPPLLGLVCGS
                     VTMIGGHGTALGFAADLARAGLPTAAVAGAAAATFGLVCGGLIGGPVGGWLIRRHHLK
                     SRVGAQIHLEAGRTGEAGILNDLRRCAGFGRPLFTHLLLLLVCLKIGAWISYFIQQTG
                     ITFPVYMGAILLGVGVRNLLDAAQLSWVDSEIIDTLASVCLGVFLATALMSLNLAELA
                     NTALPMLLILTVQVAVVAVYAGWVVFRLMGRDFEAAVMAGGLCGFGLGATPNAMANMK
                     ALVETFGPAPRAFLVVPLVGTFLVDSVNALNITFFLNLLK"
     gene            17721..17930
                     /gene="rpmI"
                     /locus_tag="PKI07_04870"
     CDS             17721..17930
                     /gene="rpmI"
                     /locus_tag="PKI07_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOC50417.1"
                     /translation="MRRPKGIKTKKSAAKRFKITARGKVLRASAGRRHLCQSKSPKRR
                     RALRGPAVVDASDEYRIKQNLPFSH"
     gene            17958..18329
                     /gene="rplT"
                     /locus_tag="PKI07_04875"
     CDS             17958..18329
                     /gene="rplT"
                     /locus_tag="PKI07_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOC50418.1"
                     /translation="MRVTNAPASRKRRARMIRAAKGFRMRRSKLYRYASDAVDHGRAY
                     AYRDRRTKKRNFRALWQIRINAAARAAGTTYSRFMEGLKAAQVALDRKILADLAATDA
                     AAFNQLVKVAQDGLKAKAAKA"
     gene            18444..19466
                     /gene="pheS"
                     /locus_tag="PKI07_04880"
     CDS             18444..19466
                     /gene="pheS"
                     /locus_tag="PKI07_04880"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOC50419.1"
                     /translation="MAGFLDQIEPLKQAALAELDGAADLAALEQARVNYLGANGRFTA
                     LMKQLGTLPREEKPAAGKAINGAKVELEAALARRRGELELKAALPAEPTDFTLPGRRR
                     ILGKLHPLTQVTEDIVRSFRKIGFVVAEGPEVEDDYHCFDALNTPADHPARDTQDTFY
                     LGGAETEAAPPLLLRTHTSSVQIRVMEKQSPPVRIIVPGRCYRRDNADATHNPTFHQV
                     EGLYVDRNVTVGDLKGTVEFVFKELMGEETQIRFRPHYFSYTEPSFEIDFSSPMTRKM
                     GREWLEIAGCGMVHPQVIENVGYDAEVWNGWAFGFGIERIAMIRYGINDIRLFYENDL
                     RFLKQF"
     gene            19472..21907
                     /gene="pheT"
                     /locus_tag="PKI07_04885"
     CDS             19472..21907
                     /gene="pheT"
                     /locus_tag="PKI07_04885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOC50420.1"
                     /translation="MKVTFNWLKQYVEFDWSPAELAERLTMLGLEVENVQKLEGEFEG
                     VVVAQVVTKDKHPNADKLNVCRVNDGQGERQIVCGAQNFKAGDKVPLILPGFSLPAKP
                     GEAPLLIKVGKIRGVESLGMMCAPQELGLGDEVSGLLILREDARVGQPFSEYLGRSGT
                     DMVYDLEITPNRPDLNSVIGIAREISAVTGNPLRLPEVNLTEASGRVADAVAVRIEDA
                     ELCPRYTARVIRGVKVAASPDWLRHALEKVGVRSINNVVDVTNYVMLETGQPLHAFDY
                     HLLAKSAANGLPTIVIRPAAEGEKFITLDGQERALTRQMLLIADETKAVALAGVMGGQ
                     NSEINPNTVDVLIESACFKPQNIRATSRKLDLRTESSYRYERGADIGICDWASQRAAQ
                     LILATAGGKLAQGLVDAWPQPFAPRQITLRHAQCDAVLGVAIPAAQQTQFLQRLALKV
                     EAGPAGEPPATTFGVPSFRVDLKREIDLIEEVARLYGVDKIPATPPRGAVGAHPHDAV
                     HDQLANVRRILTGLGLYEAQGQTLVSDVVARLTHGSALVPLSNPLSSDMNVLRPSLLP
                     GLLAALRHNVSRKINDLALFEVGRVFLESEGQRREERRVALALTGQRHPLFWSGEERE
                     ARFDISDLKGLLEEFLEQFGVRGMNYVRRPESTALFLESATVHLGRFQLGEFGLLLPS
                     LARQHDLRDAVLLAELNLDLLLARRNPNKSFRPLPAFPAIRRDIAMILPAATTHESIL
                     QVIKQAKPANLEAVELFDVFRGKNVPAGQKSMAYAFTYRHADRTLTDAEVNAVHDKLV
                     VHLKQRLQATVRE"
     gene            22444..23355
                     /locus_tag="PKI07_04890"
     CDS             22444..23355
                     /locus_tag="PKI07_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC50421.1"
                     /translation="MLQPAAMKINRRQFIQVSSLSLGAVSLAPEWSPGAAASGPVSFI
                     VRPPRMHLGLVTYNLAQDWDIPAIIRNCETAQFEGVELRTSHAHKVEVNLTREQRQEV
                     KKRFADSKVQLVGLGSTFDYHTPDQAKLRKDIEATREYLVLAHDVGAHGIKVRPNALP
                     PEVPVAKTLAQIGRALGELGDFARDHGQVIRLEVHGAGTSFPPHIKTILDTANHPSVG
                     ACWNSNPTDLDGEGWDHNFDLLKDKIFCVHMRDLFIEDYPFRKLLTRLNAINFTGFCL
                     AEIPASADPVRVMKYYRALWLAYQELL"
     gene            complement(23640..25283)
                     /gene="ggt"
                     /locus_tag="PKI07_04895"
     CDS             complement(23640..25283)
                     /gene="ggt"
                     /locus_tag="PKI07_04895"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOC50422.1"
                     /translation="MMSLLLAAVSATGAEAAHGMVASVHPLATDAGVRVLKEGGNAVD
                     AAVAVALTLGVVDTDNSGIGGGCFMLIHRADGSLVAIDGREMAPAAATPDMFIREGKA
                     DTTLSQVGALASGVPGALAAYEFAVTHHGKRKLADLLLPAADLAEKGFPLNAGYARCL
                     QRVAKDLARFESSRAVFFANGKPLAKGAILKQPDLAATYRSIAAGGSDWFYRGPFAQA
                     VQRWMRENGGILTAEDFRNYQVCLREPLRSRYREYSVIGFPPPSSGGIHVAQMLNLLE
                     PFNLKTLGEATRLHVIAEAMKLAFADRAYWLGDPDFVNVPRGLMDRTYAATLARRINL
                     ERTTSVTAHGSPPGWETDVFRKHTTHFSVADQEGNWVACTATINTSFGSKVVIPGTGV
                     VMNNQMDDFSVQPGVRNYFGLVGGHANAIAPRKRPLSSMAPTLVLKDDKPIIALGAAG
                     GPRIISQVLLELVCLLDLEMDPQQALAQPRIHHQWSPDELMIEKALPQNLRTELVRRG
                     HKLNELSSIGVSQIVARSRDGKAFVGAADPRAGGKAAGW"
     gene            25543..26790
                     /locus_tag="PKI07_04900"
     CDS             25543..26790
                     /locus_tag="PKI07_04900"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOC50423.1"
                     /translation="MSDSYIQNLIAERIGGRQYGKGAAGYKFEKIKQARRAAMAAHPD
                     GEILDFGIGEPDEMAYPEVVDKLCAEARKPENRGYADNGGVVLKEAAARYLERVCGVK
                     GINPETEVIHSMGSKAALAILPAALINPGDYALMTVPGYPVFGTHAQYLGGRVYNLPL
                     TEDNGFLPDLDSVPAPVLQQAKVLVLNYPNNPTGATATPQFFAQVVAFARKHRLVVIH
                     DAAYAALVFEGRPLSFLATPGAREVGVELHSASKSFNMTGWRCGFVAGNELLVRAYGE
                     VKNNTDSGQFLAIQHAAAHGFDHPEITARIAARYSRRMDDLVKVLGTAGFQPRKPKGS
                     FFLYVKAPKAAVSPAGARVEFQTAEEASQWLITEKLISTVPWDDAGAYLRFSVTFAAR
                     DRADEDRIFHEMARRLGEGQFEF"
     gene            27083..28678
                     /locus_tag="PKI07_04905"
     CDS             27083..28678
                     /locus_tag="PKI07_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010916247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOC50424.1"
                     /translation="MKTVTLWRRLNVAFWLLVVLLLASVGLALWVSGARSEADRRSEQ
                     LAAARATITYKVVFISDTVRGLLLAPQDTVEKRHKREAENDLAAQLNFIRSEFAGHLA
                     LLRAVSNLSDFTSHTLADFHRLVLETAENDPAGAIALYNRDYPAIRDERGKLFKELAM
                     EIQHVQDQESAASRTLGWAGLAPLVIILLAIPIVGRVHSHTVTRPLNRLVAALERMRQ
                     GDFTERLEVRRRDEFGLLSEGLNRLADNLSELVGQVQRSGNQVSGTATEIAAAAREQQ
                     GTSREIATTTAEIGTTSRRISATSQELVRTMNEVSQVAGQTADLAGSGHSAITRMEAT
                     MLQIMEASGSITAKLAVLSEKTANINSVVTTITKVADQTNLLSLNAAIEAEKAGQYGL
                     GFAVVATEIRRLADQTAVATYDIEKMVKEMQSAVTAGVMSTDKFSEEARRGIEEIRRV
                     GTHLAQIIHQVQTLTPRFQAANEGMHAQAAGAQQISDTLTQLTEAAQQTAESLRQSNL
                     AIEQLNGAARSLQASVARFKLSS"
     gene            28686..29168
                     /locus_tag="PKI07_04910"
     CDS             28686..29168
                     /locus_tag="PKI07_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOC50425.1"
                     /translation="MVFLLFQLGSGRYALEASRVVEVVPLLEMKPLPQAPRGVAGIFN
                     YRGRPVPAVDLSALTLGQPASERLSTRIIIVHCPDESGNDHLLGLIAEHATELLHKEP
                     SAFIDSGVKLSRAPYLGPILMGSPGPIQWIYEQRLLPEAVRNVLFAETVVSQLPDGPA
                     "
     gene            complement(29208..30116)
                     /locus_tag="PKI07_04915"
     CDS             complement(29208..30116)
                     /locus_tag="PKI07_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC50426.1"
                     /translation="MRKLACFTLTLLACASLPGQDVPKAAAAADQLGWQMAIHAYTFR
                     KFSIFECIDKTAALGLKHMSLSGSVSLDGKSGRKTVTLSDADAAAIRKAAAAKGIKLI
                     NIGVVQLPPDEAESRGVFEFAKRMGIDTLVAEPEPAALDVVEKLCKEYAIKVAIHNHP
                     KPSRYWNPDTVLEAIKGRSLLLGACADTGHWLRSGLDPRECLKKLEGHVICLHFKDLV
                     PEEPKTAAAAEAGKKKEGAAKAMHDVPWGTGVGQVKAQMAELKRQGFRGAFCVEYEYN
                     WDNSMPEIAECKKFFDATCAELAGKP"
     gene            30361..31272
                     /locus_tag="PKI07_04920"
     CDS             30361..31272
                     /locus_tag="PKI07_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC50427.1"
                     /translation="MKLGFVTAILPELNLNQVLEFAAAERFACVEVMCWPVGKAERRF
                     AGVTHIDVDGFTRARAEDVNALCARHGVSISALGYYPNPLDPDPAVARVAVSHFRKVI
                     LAAEMLGLKNANTFVGRDWTRTVDENWPRFLKTWKPLIAFAEDHGIKVGIENCPMSFS
                     RDEWPGGKNLATTPVIWRRMFNEIPSRCFGLNYDPSHFVLQQMDPLSPLREFKSRLFH
                     LHAKDMMIRPERLNEVGIFAFPKEWHTPRIPGLGDIHWGRFMAALYEIGYDGPVCIEV
                     EDDTFGKTLAGRKQALRIARNALEPFF"
     gene            complement(31371..32126)
                     /locus_tag="PKI07_04925"
     CDS             complement(31371..32126)
                     /locus_tag="PKI07_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HOC50428.1"
                     /translation="MNKTRMTPRGAAPRPFVLMNMAMTADGKIATANRVVSSFGSRRD
                     QEHLYELRATADAVMAGARTADLNAVHLGPGALKYRRLRQRRGLAEFNLRIIVSRTGS
                     VNPAARIFQHTFSPILVLTTQRAPARRRTRLRALGAEVRVCGAKEINFPAALRWLRRQ
                     WGIKRLLCEGGGELADALFRAGLVDELHLTVCPRIFGGRAAPTIADGRGAGALARAAR
                     LELHSARRVGDEMFFVYRAPKRRATPRLGRGKP"
     gene            complement(32123..33223)
                     /gene="queA"
                     /locus_tag="PKI07_04930"
     CDS             complement(32123..33223)
                     /gene="queA"
                     /locus_tag="PKI07_04930"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOC50429.1"
                     /translation="MRTADFEFELPPELIAQAPAPERDQSRLLVLHRDSGRLEHRRFP
                     DLIEYLHPGDALVLNNSRVIPARLRGVNALTGGQFEALLLEENAVNDWWAMLRPARRA
                     RTGTRIQIQDARARPSGVQAVVIEANAEGHRRLRFAGTPNVLERLNTLGEVPLPPYIA
                     RPNGSRLESDRERYQTVFARPGGSVAAPTAGLHFTEALLAQIAARGTQVCFLTLHVGL
                     GTFAPVKAETPAAHRMHEERYELSAATARVINAARSAGRRVIAVGTTSLRVLESQAAQ
                     SPPPGGTRPRAAVTAGHGRTRLFIYPPRDFRAADALLTNFHLPRSTLLMLVSAFAAPN
                     STRGRERMLAAYAEAIRERYRFFSYGDAMLIL"
BASE COUNT         6312 a        10801 c        10464 g         6240 t
ORIGIN      
        1 accggtagca ctggatcatg atcttggggt tgctggcctt catggagaac ttgaaattgt
       61 ggaagtcgtg ctggcgcgca atgcgggcaa actccagggc gctctccacc atgccgagcg
      121 gcgaatcgcc aaagcgattc ataatgcggt cgctcagcga gccatggttg gtgccgatgc
      181 gcatggcgcg gcccagcttt ttgcacaact cgaccagcgg cgtgaacttt tctctaatcc
      241 gcgccaactc cgccgcatac tgcgcctcgg tgtattcctt gacgataaac ttcttcgagt
      301 cggcgtagtt gccgggattg atgcgcacca tctcgaccca gcgcgccgct tcgagcgcgg
      361 cctcgggctt gaaatggatg tcggccacga tgggcaccag gcagccccgc gcgcgcagcg
      421 ccgccacgat gttttcgagg ttggccgcgt ctttaaccgt gggggcggtg atgcgcacga
      481 tctggcaacc gaccgccacc aaatccagcg tttgccgcac gcattcggcg gtgtccatgg
      541 tatcgcaggt gagcatggat tgcaccacca ccgggtggcg gccgccaatg attacccccc
      601 cgcgttgcgg atcgccgacg atgacctcgc gcgtcaggcg gcggcgcaaa aatccgggcg
      661 agtcacagtg cggaaccatg ctcagcgccc tccgtccggt gcgctttccg gtttctcgat
      721 ggtgacctcc tgtttgaaca tcgagcgaaa aagcgagaac cgccgcacat cgttaaacga
      781 aacatacagc atgaagccaa ttagcagcat ggcaaaggcg gtattggtat attccaccaa
      841 ccggcctggc agtggccggc cgaacaagcg ctcaacgcag gccatgacga tatgcccgcc
      901 gtccaacacg ggcatgggga agaggttgat gatcgccagg ttgatattga gcaggacgag
      961 gaaacttaaa gccaggcgcc aatccgagtt cacataggcg gccaatatgg ccaggatgcc
     1021 cggaggaccg ctcaggtctt tcaggccgac cccggtttgc ttggaatgga agagcgcgga
     1081 gaaagtgctg atcgtccgct ccagcaccga ggcgatctgc gtccagggcg aaatgtgctc
     1141 aatccgatag accggcttgc cggtgccgat ttcagctcca attcgcccca cctttacccc
     1201 ggggtccagg cgcggtgtga cctggagtgt ttggcgcgcg cccgcacgcc tgataatgat
     1261 gtcatgcgac tctccggggc gcttctgcac gagtttgatg aattggtcgg ccccgtgaag
     1321 cggcacctgc gcgaacgaaa ggatttcgtc gccggccttg agcccggcgg cttccgccgc
     1381 gctcccggac aaaacccgcc ggatcaccgg atgatcgcgc gggtcgagat tgagaacctt
     1441 cagccccagg gcttcgctga ccaccgtgtt taattcgtag gtattggtga attgcccgac
     1501 gcgggcaatg accaccgtca gattggtgct gcgcgccaga atggtggcct cctgcacttc
     1561 ctgccaggaa ctgatcggct tgccttccac ggaaacaatc tggtcgcccg gctgtatccc
     1621 gagcctggct tcgcgcgagt ccgggtccac atagccgatg atgggcggat tgacctgcac
     1681 gggaattccc acaacccaca acaggcaggc aatcgctatg ccgaagagca cgttcatcag
     1741 cggcccgaaa aaggcgacca ggattttgga ccacggcgac gccggcggca ggggagggct
     1801 gttttcgtcc gccttgcctt ccagggcgtc ggaggttgcc atctgcggca gttttacgaa
     1861 gccgcccgca ggtatccagc gccaggacca ctccacccca tcgcgcatcc agctcactat
     1921 tttcgggccg accccgattg agaagccctc aatcttaagg ccgcgacgcc gcgccatcca
     1981 gtaatgtcca tactcatgga caaataccgc cgcgccgaag agcaggatta ctgccccgac
     2041 cacatacaca atattcagaa gccacatatc cggcaggaaa gaagctaatc cgactcgttg
     2101 cgaatgtcag ttaattattg cagggcccgg tctggccccg ggcccggcgc cggttggctg
     2161 ggcgttcatc cccgttccgc ttcgtggcgc gcccattggt cggcctcaat aatttgctcc
     2221 agcgttgggt gcggaattac cgggtgccga tccatgacgc ggcgcaccac ggccgaaata
     2281 tcggggaagc gaatccgccg gctggcgaag gcttcgaccg caacttcgtt ggccgcattc
     2341 aataccgcgg gcagtgtgcc gccgatttcc ccggctcgtc gcgccagggt cagcgcgggg
     2401 aaacgttccg ggtcgggctc ttcgaacgtc agttggccca gcttggccag gttggtttgg
     2461 acccggttgc tgggcgcccg cgccggatac gtcaaggcat actgaatggg cagacacatg
     2521 tccggcgtcg aaagctgcgc caggatcgag ccgtccacga attccaccat cgaatgcacg
     2581 acgctttgcg gatgcacaat caccgcgacg cgcgccattt ccacatcgaa cagccagcgc
     2641 gcctcgatca tctccagtcc cttgttgaag agcgttgctg aatcaatcgt gattttgcgg
     2701 cccattaccc aggatgggtg ctggagcgcg cgctcgatgg tgatgtccgg gaatgcttcc
     2761 cggggcgtgg tgcggaacgg ccccccggag gcggtcaacc acagccggcg aacggattcc
     2821 gcgggcttgc cctccaggca ctgaaagatg gcggagtgtt cgctgtccac cgtcagcacc
     2881 cgcacgccat gcgcctgcgc ttcccgcatg acaatctcac cggccatgac caggatttcc
     2941 ttggacgcca cggcaatatc cttgccggcg cggatggcag ccagagccgg ttgcaggccc
     3001 gcggtcccca caatggcaat caacacaata tccgcctcgg gcagggtggc gagcttcacc
     3061 aacccctgcg cgccggagaa caccgccatg ccgccgccaa gcagatcgcg cagttcctgg
     3121 gcccggctcg ggtcggcgat ggaaatagcc gcgggctgga gcttgcgggc ttgtgccagc
     3181 agcaattccg cgttgttgcc cgccgccagc ccaaccaggc ggatgcagtc ccgcaaatct
     3241 tcggccacct tgatggtgct ggtgccaatg gagcccgtgc agccgagaag aacaacgttg
     3301 cgcatgaaga aaagtcccgg acttaacgct cagataaaac gtgccgcagg tagagataca
     3361 taatcggcgc gttaaacagc aggctgtcca gcaggtccaa tatgccgccg atgccaggaa
     3421 aaaagctgcc cgaatccttt gcgccggcct cgcgcttaaa aagcgattca atcaggtcgc
     3481 ccaccaccgc gcccatgccc aaaaccaccc ccagaatgag ggcatgaacc cagttcatac
     3541 cgggcagctt ctgccccaga aaatgcgcca gcaacaggct ggccagcgtc gaaatcgcta
     3601 tcgcgcccac aaatccctcc cacgtcttgg cggggctgat gcgcgggcat agcttatgcc
     3661 gtccaatcaa ggatcccaca gcgtaggcgc cggtatcgct gaacttggtg ataacgatga
     3721 agaacagcag ataatagcag cccgccgtcc cggggtagaa gaagaaattg atcttttgaa
     3781 cgaaattcag cagccaggcc acatacatca agcccaggag cgtcagggcg atcgtcagca
     3841 atcccgccgg gtggcggcgt gagaagagct ggcacaggca gaggcccagc acaaaaacga
     3901 ttagaaagct ggcctcgaag tcgttgacgc gcgctggcgc cccggaagtt cccaacaatc
     3961 ctgtcaggtt caggaaagtt cccaccatga gcaagacacc tccggttacc ccccagtatt
     4021 tgaagcagcc caatccgcgc ttggcggcca gcccgtagaa ttccagcaac cccaagccgg
     4081 ccagcagcac cattgccagg agaaatacat agtccgacac cgaccgatta ctcgagaaca
     4141 gcgccgtcag gaccaccgcc cacaacgcga taaagctgcc caaccggcgc agaaacacct
     4201 gggacttgga cagcgcaact gccgtcgctg gcggcgtcgg ggcggtctta tcaagcatgg
     4261 ggccagagac taggaaatcc tttcttcgtg tcgagccaat tcctggcgga cgcttagccg
     4321 tgcatgcacg gatgattgcc gcgtttcgag cgtgatcaga tatttccgcg cgccgcaaag
     4381 accgcgcaag aacctcaacg ataaggtctc gatgcgccca cgaatggagc ctgaccggcc
     4441 tccccaaaaa gtgaaaataa tccccgcctc catccctcta ccatcatttc ttcgcgatgg
     4501 ccgatttttc tattcggtgg cgtaccttaa ggctatagtg ccgctaatag gccatgacat
     4561 cacaaaccac acttaatcca gaagctgggt ctcaattcca tcctgcaagt cccggttccg
     4621 gttcgggcaa ggagggccgg gttcggcgtt cgaggttcgg acttttaggg gaactcgcct
     4681 cgcgggtttc gagcttcatc ggtctgtgtt tcgtgggggt tcctgctttg ctgctggctg
     4741 ccgatgctgg cccctggcca gtttccaagg ccaatgactg gctggccaaa caaggctggc
     4801 tcgtcggctg caacttttct cccagctcgg ccatcaacca acttgaaatg tggcaggcgg
     4861 acacgtttga tcccgccacc attgaccgcg aactgggctg ggccgaacag cttggcttca
     4921 acagcatccg cgtcttcctg caccatatcc cctgggagca ggatcgtgcg ggttttctca
     4981 agcggctgga tgaattcctg aacttggccg ccaaacacca tattggcgtc atgttcgtgt
     5041 tgctggacag ttgctggcac ccctttccca agccgggcaa gcagcccgcg cccaagccgt
     5101 tcgtccataa ctccagttgg gtgcaatgcc cgggactgga cattctgaag gatcccgcgc
     5161 gccacgacga gttgcgcggc tacgtcaccg gcatcgtgcg ccatttcgcc accgaccgcc
     5221 gggtgcaggt gtgggacatc ttcaacgaac cggaaaaccg gaatcacatc agctatggcc
     5281 agtatgaacc ggagaataaa gccgaactgg ccctggcgtt gatcaaaaag gcgtacgcct
     5341 gggcgcgcga agcgaaacct tcccagccca tcaccgcggg ggtgtggatg ggcaactggg
     5401 ccgatcccgc caaactgtcg ccgatggaga aattctgcct ggagcaatcg gacatcatca
     5461 actttcacag ctacggctca ctgccggtca ttcagaaatg cgtgcaaaac ctgcgccgct
     5521 atcaccgccc gatcgtctgc accgaataca tggcccgtcc gcaaggcagc acgttcgatc
     5581 ccatcctcgg gtatttgaag gagcaaaaga tcgccgccta caactggggc ttcgtggcgg
     5641 gcaagacgca gaccatcttc ccctgggatt cgtgggaaaa gcaatacacc aacgaaccgc
     5701 cgctttggtt ccatgatatt tttcgccgca acggccagcc ctaccgtccg ccggaagtgg
     5761 agtacatcaa gcgggtgacg ggcaagccgt gattgaggga ggtgcccaaa gcaaacggtg
     5821 gcactcgcat aattgcgttc gccgccgatg ttttgcgcaa aggttcgccc ggtttagcca
     5881 taacgcttgc aagtccagcc gtggcgtgtc ggcacccggg acgggactcg gttgccggag
     5941 gttgcggtaa ttgaagcatt tggtccgcgc agtgtggatt gccgaggata gaaagcccag
     6001 tctgcggaca tgccgttaat ccttatttta tcggcttttg ccgagcgtcg gctggattat
     6061 ttcggggctg tggcggaaat cgcgtataat cccggagaaa ccgagtgggg cttgtcccat
     6121 gtttccggat tggatactga ccgggcgttc ccgcagaatc caagtgagtc ggttgggcaa
     6181 accgcttaag atgtgaggcc atgcggcgag tggtgtttag agagatggtt ctgcagcgtc
     6241 ctcgtttttc ggggctggcg ccccgggtgc ggcgcgtttt gctcgctttg tttttgctgc
     6301 aatttgccct ggtttgggcg cgggtatggt ggccggggct gctattcggc cacgctcgct
     6361 ggccggacgg gcttttgctg gtgctcgcca atgccgtctt gctcgccgcg ttaacctgcc
     6421 agttgcccgg ccagaatgtg atgctggccg cgctcatcat cgccgtcctc gctggcgcgg
     6481 tgcaatgcct gaacgcgctg accgccatac cattcggccc ctgcgtttat accgggaaca
     6541 tcggccggga actctttccg ccgctgccct gggcggtgcc gctgatctgg ctggcggcca
     6601 tcctggcctc gcgcggcacc gcccggctga tattgcggcc ctggcgcaag acccggaact
     6661 atggtttcca gatgatcggg gtcaccactt tactgacggt gctgctggat gcgggcctcg
     6721 agccgtttgc cacgcgcgtc gcgcattact ggttctggaa tcccgtcaaa ctcaagttcg
     6781 gctggtacac ggcgcccggc gtgaacttcc tggcctgggc ggccaccacg ctgctgattc
     6841 ttgcgtttgc cacacccgcg ctcatcaaca agcaaccggc gcgcgggtcg gcgccggatt
     6901 attcgccgtt gctcgtgtgg cttctgttca tgctgctttt tgccaccggc gccgccggcc
     6961 atcaattatg gccggccttc ggtttaacct gcgccatcag catcacggtg gcgggggtgg
     7021 cccttcgcgg cgcccgctgg taacgggccg ccgtcagcgt tttggggtgg gcagcgactt
     7081 gaggaaagac tgcgtgggga atcgcgcgcc ggggcagcgc gtgtgccgga cattgacctg
     7141 ttgatgcgtc ttgaccgctg tgggggtgag ccggcagcgg gtcatcagcg cctccaccag
     7201 caatcgcagg ctggtcattg ctttggggga gggcgggcgc ttgtcgaaat tgccgaccag
     7261 gcagatgcca atcgccgtct tgttttgcgc cgcactggcg aggtggccgc catccagttg
     7321 ccgggtccag cgttgtccga tggcaatcgc tccgtcgccc atgccgttgc cgttgcctat
     7381 aacgaaatgg taagcgagtc cattctccat gcgccgcacc tcgcgatgat agcggtccat
     7441 ggtcctggcg gtgccggtat cggtgccgct gtggtggatg acgatggatt tccaccggcc
     7501 ggcccgcacc ggcgccttgt caatcgcctc ccgcaccgcg cgcggcagca cactgggcgg
     7561 cggtggcttt gcggcgggct ttttcgcggg ggcaacgcgc cgcgtcggga ttcgcagccg
     7621 ctggccggcg gagatgtaga aattccggtt cagcccattg cgctcggcca gcacggccgg
     7681 agagacgcca taacgccgcc cgatgctata gacactatcc ccgcgtttga cgatgtaaat
     7741 cgtctccgcg gccccggcag ccattggcgc ccccaccgcg cacagcaaca gccccagagc
     7801 tacccggata atcgccagcc gggcacgata atccctgttc aaacgccggt ccatgttggc
     7861 tcaggctagc ctcgatttgc ccgcgctggc cagccttttc ctgccctgat aatctcccgg
     7921 acaaactcgt acgtttcgcc ttggatacgc gaggcgaaga gggcggctgc ggttcctcag
     7981 cgcgttcttt tgcaagcgcg ggaacaggcg ccaaagacag gcaaggcgct ggcttatggg
     8041 ggccatgcgc ggacgaagcc gggccagtct ctccccgaaa ggagttagcc actaagcccg
     8101 gctttcgggc tgcccgtcgt cgtgcgggtc acctttacgc gggcgaccag catccccgcc
     8161 atctcctcga cttgcagttt gcaggccccg agcttcaaca cgtcgcccac tttgggaaaa
     8221 ccatccagcc gtcgcgtgag aaagccgctg actgtggaga tgccttcttc acgcagcggt
     8281 tcccccacca gttcctccag ctcatgcaag ggcagcgttc ccgccgcttc ccaggtgttt
     8341 tccccggccg gcaccagcag cggcttttcc tggtcaaact catcctgaat ctgccccacc
     8401 aactcctcga ggatgttctc cagcgtcacc atgccagccg tgccgccgta ttcatccacc
     8461 acaatggcca ggtgcagctt ccgttccagg aagaactgca gcagcttctc cagccgtgcg
     8521 gtctcgggaa tgtagatcag cttccgggcg accggcagca agtccgcccc gcgccgcgcc
     8581 ttggtgcgca tcgtataaag gtccttgata tgcaccaccc ccagcgtccg atcgaggttt
     8641 cccccttcgc acaacggcag gcgcgagtaa tgcgtctttt ccgccacgtc caggcattca
     8701 gcgatgctgg cctcggtatc aagcgccaca atctcatgcc gcgggcgcat cacctcgcgc
     8761 gcgacccgcc gccgcagatc cagcgcgttc agaacaatgt cccggcccag ggccgagccg
     8821 cccgcctgct tttgcgcggc ccacaacagc aggcgcagtt cctcctcgga gtgcgtgcgc
     8881 cccccttcgc tggtcggccc gaggccaacc tggcgcagca tccactgcgc cgaatcattc
     8941 agcagcacga cgaaaggata ggaagcgcgg tagaaccaga gcaacggata ggacacccac
     9001 aaagtcgttg ccagcggctt ctgaatggcc agccatttcg gcgcctgctc cccggcgctg
     9061 atgtgcagga aggtgatgat ggaaaatccg accagaaagg ccacccactc gcgcagttcc
     9121 ggcgattcca cgcccgccag gtcgaacacc ggcttcaaca gcacggcgaa caccggcgcc
     9181 ccgatccagc ccagcccgag gctggtcagc gtgatgccca gttgcgccgc gctcagaaac
     9241 gaatcgagcc gctgcaaaat aaataccgcc acctgcgccc gccgctgccc gtcgcgggcc
     9301 aggggactga gttgcgtgtc ccgcaccttg accaggccga actcggcggc gacgaagaac
     9361 ccattcagca gaacgagcac gatgacgccc agcacgtgca gggcaatgga aagcagggcc
     9421 tcgccgttca tgctataaac tcacctcccc gaagatcagc ttgaggacat cctgcaggct
     9481 caggatgccg atctcgcgcc ggtcgcgccc cagcacaatc gccagccgct gcccgctccg
     9541 ctgcaagcgc cgcagcgcca cctccaggcg caggtcttcc gccagaaaga acggcggctt
     9601 catgtagtcg ctcaccggcc ggcgctcatc cagggccggc tggtaaagca gcgagttcac
     9661 cgccagcatc ccgatgatcc gctgctcgcc gccgcgcatc tcccacaccg gcaggcgggt
     9721 aaacttccgc tcccggcaca gcgccagcgc cgcgctcgcc ggcgtttgcg ccgccagggt
     9781 gaccgcctgc tccaggggcc gcatcgcctg ccgcaccgtc agcgcctgca aatccagcac
     9841 gcggttgatc atctggcgct cctcggaggt cagcacttgc gcggactcct gcatcagcag
     9901 gcgcacctct tcgcggttgc caaataaatg gccggtgaac gccttgccgc cccgccagcg
     9961 cagcagcgcg ccggagaacg cctccaccag cgccaccagc gggcgcagcg ccaggtggat
    10021 cagccgaaac gggcgcgcca gcgccacgca cagccggttc ggatacctcc ggaacagcat
    10081 cttgggcagc aaatcaaaga acgtgtagaa caggaatacg gccaccacga acaccccgaa
    10141 gaaccacagc cgccggccgg ccagggccgc gtgcaactgc accaccagcc agcccaggat
    10201 caggaagttc gccagggtgc tgcccaccag aatcgtccag agaaagtttt ccgggttctc
    10261 caggtaatca tgcagcacct tggccgaggg cttcccggcg cgcatctgcc ggcgcaggcg
    10321 caggcggctc aaggcaaaca cccccgcctc catgcccgcc aatacaaacg acagccccag
    10381 gcacgcgccg aagatcgtcc agatgagggt gtgggccgtc atggggtttc cgggcgttaa
    10441 cgcatgactt ccaccagcaa ctcccgcacg cgccgctcgt cggccgcctg cgccgtgagc
    10501 cggagtccgc tgaacgtcgc cgcatcgccg cgcgccggca ccacgtccag caggctcgcc
    10561 agcagcccgc ccatcgtttc gacttccggc acatccgcca gcgccgggta ttcccgccgg
    10621 aaatcgtcca accgcagcgt gccggccacc cgccagcgcc ccggctccaa tcgttccatc
    10681 acaaacccct ccgcctcggc ctcgccctgg attttgccga tcacttcctc gaggatgtcc
    10741 tccatcgtca caatgcccgc cgtgtcgccg aactcgtcca gcacgatcgc cagcccgcgt
    10801 tgctgccgct gcaaggcccg gaacagttgc agcagattca tcagctcggg cacgaacgat
    10861 ggcagctcaa tggcatccgc caggtctatc tgcgggttca gcagcaacgc ccgggtgttg
    10921 agcacgccca cgatggtatc ggtctcctca tcataaagcg gcagccggcg atgccggtat
    10981 ttgcgcgccg cggcgatcat ctcctccgcg gacaggtcgc tggagatggc cgccatccgc
    11041 gaccgcggct tcatcacttc ccgggcggtg cgctggtcca ggttgatgat ttgcaggata
    11101 atctccttct cggattgcgc cagcgccccc tgctgaaagg ccatctccag caattcctgg
    11161 tagtccgcat ccgtcacaac ggctcccggc gacccggtcc ggcgcgcaac catccccagg
    11221 atggccgtgt tgaccttctg cgccccccgg cacagcggca ggccaaaccg cagcaggaac
    11281 gccatcggcc gtgccacccg caaagcccac tgctccggcc gccgcacggc cagcgttttg
    11341 ggccacacct cgcacccgat caggttcaag accagcaatc ccgccacggt cgccaccagc
    11401 ggccattgcc cgtggatggc ctcccacagc gccaccgcca gcatcgccgc gctggcaaac
    11461 gtattcccca gcaccatcgt cgccagcaaa tcctgcggtt cggccagcaa ctgcgccacg
    11521 gcgctccccg cccgggatgg ccgcccggcc agttggcgca cctgccattt actcagggaa
    11581 aacaacgccg tctccgcaag agcgaagaag aagctcgctc ccgcaaagat caaaactgcc
    11641 actgcggcta ctagcggccc tgacgccatg ccccgagcat gaacctgaaa tccctccaaa
    11701 gcaagccgcg gttttagacg ccaactccac gccccaatcc atgcccgcct ttgcccagct
    11761 cgccgttcat tatcaaggca ggggcgcccg gctggcgcga gggttagcga ttcaatcacg
    11821 ttcctcccca ttctccggca actcagggaa aactgcttag cccgtcgagt tcacgcttaa
    11881 cgccgtgcaa aaatatgtcg tcggcatgga aaagaggggg cgcgggcagg ctgcgcaccc
    11941 tcatacggca tacggttgcc gtttcctgca gggagccaaa caggcaagtt ctgaagcttc
    12001 ggtcccaccc acctgcctcc gacttccgct ttccccctca gccccgcagt ttcaacggca
    12061 aaaaccaccc tgaaaaccgc ccccaaagcc gcccggactc tcaataccct caaaaagcca
    12121 caactccttg taatcacgcc actttcaccc taccttcaag ccgtgtacaa ggggtgtaca
    12181 agggatgctc accgggtgta caacgataaa tcttatgtac ctccggtgag catcccttgt
    12241 acaccccttg tacacggctc gatagtggct atggatgagg ctggaaagac gggggttatg
    12301 taaaaaacgg ggggctcctc acggttccgg caaaataggc cggaaggcta tgccagcagg
    12361 ctttgtccct ggggcatcag ctatgatcgc cgccgggggt ggccccgggc cggaaacgct
    12421 tatggaattg gcaccttgac gggcgggctt atttcaagtt tgccgggttg agcggcttca
    12481 agtcctttgg cacaaacagg ccgtttttgc ggatcaggtg gtcgtcgaac cagatttcgc
    12541 cgccgcccca gtcgggccgc tgaatcagca ccatatccca atgcaccgcc gaacggttgc
    12601 cattgtcgca ttcctcatag gcctgccccg gggtcaggtg cagggagccg gcgatctttt
    12661 cgtcgaacag gatgtcgcac atcggctttt ggatcagggg gttgaggccc aaagagaact
    12721 cgccggtgta gcgcgcgccg gggtcggtat ccagaatctc gttgaggcgg cgggtgttgt
    12781 tcgccgtggc tttgacgatc cggccggctt tgaattccag gcagacgttc tcgaatttgg
    12841 tgccggaata cagggtgggc gtattgaact gaatgcgccc gttgatggag tccttgacgg
    12901 ggcaggagaa cacctcgcca tcggggatat ttcgatcccc tttgcacatc tgggcaccca
    12961 tgcctttgat gctgaacgcc aggtccgtgc cggggccctt gaggcggacg cggtcggtgc
    13021 gctgcatcca gcgcaccagc ggcgtcatgg cgcggcccat ccggcgataa tccagcgtgc
    13081 agacttcgaa gtagaaatct tcaaaggcct cggtgctcat gccggcggcc tgggccatgc
    13141 tgggggtggg ccagcgcagc acgcaccagc gcgtcctttt gacccggtag tcgagcaccg
    13201 gccgggtgat gcggctgtag agcgccatct gatggccggg cacgtccgcg ttttcgctgg
    13261 cgttgtcgct gccgcgaagg gcgatatacg cctgcatctt cttcatccgg aacagctcga
    13321 agtcccgcag caggcgggcg tgccggggat ggatatcgcg cagaatctcg cggttgacgc
    13381 gggtatggcg gatttcgatg aaaggtgtgg cgcccgccgc ccgcgccgcc cgcatgagtt
    13441 ctatggcaaa cgcatcgggc acatcaatca tatccagcag gacgcaatcg ccccgcttca
    13501 gggcggtcga gtattcgacg agcaactttg ccagttttcg ataacgcggg tcagtcatag
    13561 cacgctggaa agaaagcaca ggcgccgccc ggagtcgagc cgggactctc aaaagggcgg
    13621 gcgcaaagcg tcgctcggtg ggagagcgcg ctccagcagg aggagcgcca tcacggttcc
    13681 cggtgctacc ccggcgaacg gcggcgcggg tttcagggcg gaactgcctt tctcagcccc
    13741 ggcccgtccg gcgccctttg cgcaagcgga ccaccgagct gacggtgaaa cagccgcccc
    13801 gccgccggat tctgaagccg cagcaggtgc cggcgggaag gctcttgatg ccggcggtgg
    13861 tgcgttgggg aggttggtgt gcggcggaaa acgcgaaccg cgcgggcggc gtcaacgccg
    13921 cgcttccggc atctgttcgc gccggcggag cgcgcgttcg cgccgccggg tttcgcggcg
    13981 cagcaaatcc tcggcggacc agccccgttc ctgcgcatat tgcgccagcg caaaaagctg
    14041 cgcggccaat tcccgccggg tgcggacggg cggggtggtt tcgggcggca acagcttggc
    14101 tttgcgcgct ttgcgcacga gtttggcggc ttgcagcagc gcgggcagcg agcgcggaat
    14161 gccgtccaga gcggaagggc gggcatgccg ggttccctgt ttttcggcgc gcttgatttg
    14221 ctcccagttc gcccagacct ggtccacgtt tttgacccgg agccggccga atacatgcgg
    14281 atggcggcgg atcaatttat ccgcgatgcg gcgggcgacg cgctcgaaat caaacgcgcc
    14341 ccgttccctg gccagttggc agtggaacac cacctgcaag agcacatcgc ccagttcctc
    14401 ctccaactcg tggtcgtcgc cggcctcgat ggcatcgagc aattcataaa cctcctcgac
    14461 ggcgtgccag cggagggtcc ggtgattttg ctgccggtcc caggggcagc cgcgcggcga
    14521 ccggagccgg gccatgatgg ccagcaaatc gttaatggcg ggagttcgtt tcatgcaagg
    14581 gggcgtgggc gggaagcgcg gcgaccgggc gggatttttt gccggggcgt tttcttatcc
    14641 atattccggg ctcgctcgcg cgggaggagc atcacaggat gaccaggttg tcccgatgga
    14701 caacttcgac gcgctggagc ttgcgggcct tgatttccgc ggcgccaaac ccggcgatgc
    14761 cgcgggcgaa ctccgtgccg tcctggtcgc aaatgcgcac cacctcaccg gcggcaaaat
    14821 cgccgtcgca gcgcgtgatg ccgggcggca ggaggctttt gcccccttcg cgcacggctt
    14881 tccgcgctcc ggcatccaca tacagcgcgc ctttggggtg atgaaagaag gcaatccagc
    14941 gtttgcggcc cttgagccgg gtgggctggg gcacgaaaag cgtgccctcc tcctcgcccg
    15001 ccatcacgcg ggcgatcacg cgtttcttgc ggcccgaggc aatgaccagc gggatgcccg
    15061 cgcgcatcac gattttggcg gcctgaactt tcgaggccat cccgccgacc gccgtggcgc
    15121 tgcgggtgcc ggtcgcagtt tgctccagcg ccgcgtccac ccgccggatc agggggatgg
    15181 tccgggaatt ggctttgccg aaattctcca gcacgccgtc cacggtggtc aggatgagca
    15241 gcaaatccgc cggcagcagc gaagccacca gggccgagag caggtcgttg tcgccgaact
    15301 tcaactcggt gaaagagatc acgtcgttct cattgatgat gggaataatc ccatgctcca
    15361 ggagcgtcac cagggtattg cgggcgttga ggtggcgttc gtggtgctcc aggtcgtcgt
    15421 gcgtgagcag gatttgggca accgccaggc catgcttccc gaaaagctcg tcatacaccg
    15481 ccatcaaccg cgactgcccc accgccgcgc atgcctgcag atcagccagc gcgcgcgggc
    15541 gctgcgaaaa gcccagcgcg cccatgcccg cccccacggc tcccgacgaa acgaccagca
    15601 cttcccggcc ggcctgccgt tgttcggcca attgccccac caattgctcc atttgggcca
    15661 gctccggctg cttccggcta tccgtcagga cgccggtgcc cagtttcacc actatgcgcg
    15721 tgatatcttt gatgatttcg gaacgcacgc caagctgggt agcaaatccg gcagcgggtg
    15781 tcgaattcca aatctgccgg aaacggccgt aaagcggtgg gtagcaattt ggcggcggga
    15841 ttccgttgtc attccggcgt tcgaggttta ggctgcgcct gattttcgcg cgatgagcgg
    15901 ctcggaaacc ctgatagtta ttcctgcctt gttcatagcc atccttgcca tcccggtatt
    15961 gctggcaggc gaatggctcg tcaaacgctt tcgcctgctc gcgcgcttca acatccccgc
    16021 cccggtcgtc ggcggcctgc tggtatcggc gctgctgctg ctgggacacc tttcgggcgc
    16081 ctttgcggcc cggtttcaaa tccacgtgac ggcgcgctgg tggacctggc tggtgacggc
    16141 ggagccggaa tggttccagg cgccgggcaa gcatgtgaac accccgtttc tggtggcctt
    16201 ttttgcgtgc attggcctga atgcccgctg ggaactggtg cggcgcggcg gcgcgcaggt
    16261 gctgcttttc tggggggcgg cggcggcctt ggccgtggcg cagaacggca tcggcgtggc
    16321 gctggccaaa ctgatcggcg cgccgccgtt gctgggcctg gtctgcggca gtgtgaccat
    16381 gattggcggg cacggcacgg cgctgggctt tgccgctgac ctggcgcgcg ccggcttgcc
    16441 cacggccgcg gtggcggggg cggctgcggc aacctttggc ctggtctgcg gcggcttgat
    16501 tggcgggccg gtgggcggat ggttgattcg ccgccaccac ctcaaatccc gcgttggcgc
    16561 gcaaatacac ctggaagccg gccgcacggg tgaggcgggc attttgaacg acctccgccg
    16621 ctgcgccggg tttgggcggc cgttgttcac gcatctgctg ctgttgctgg tgtgcctcaa
    16681 gatcggggca tggattagct atttcattca acaaacgggc atcacctttc cggtctatat
    16741 gggcgcgatc ctgctcggcg ttggcgtgcg caacttgctg gacgccgccc aactgtcctg
    16801 ggtggacagc gaaattattg atacgctggc ttcggtctgc ctcggggtgt ttctggcaac
    16861 cgccctgatg agtctgaacc tggccgagtt ggccaatacc gcgctgccga tgcttcttat
    16921 cctgacggtg caagtagccg tggtggcggt ctatgccggg tgggtggttt tccggctgat
    16981 gggccgtgat tttgaagcgg ccgtaatggc tggcggtctc tgcggcttcg gcctgggcgc
    17041 cactcccaat gccatggcca acatgaaagc gctggtggaa accttcggcc cggcgccccg
    17101 cgcgtttctg gtggtgcccc tggtgggaac gtttctggtg gattcggtca acgcgttgaa
    17161 tatcacgttc ttccttaact tgctgaaatg aggaacgccg attggcgtaa ctggaggtac
    17221 tattatttaa gtgcttatcc gaggcgcggc acagttagcg cgccgggagt ggaagcgcct
    17281 gggccgggcc aactcgggcc gggacgatgg aagcaacttt gatcccagcg gcagccggac
    17341 ttaaacggcc ggcaggcaat gggtcagtcg cgaacggcaa tttccaaatg ggcccggcgt
    17401 ggaatcggcc aggcaagttg gtgctgggtc cggcaaatga tcgaggcggt ttgccttggg
    17461 ccgattcaaa gccgccgccc ccagggcgcg ccctgtttcc cggtggggcg attgcggtcg
    17521 gcggccggca tcgggccaat cgcccgaact gttccgggga tagcagagat tatcagcagc
    17581 gcggcggttc ttcgcagagc cagccggggc tgccatttat tcccggccgg gtattcgatg
    17641 caaatctcaa aaatacgctt gattcgtgag tgtgcgcttc gtatattgcc cgctcttttt
    17701 aagaaaaagc gaatagattt atgcgacgtc caaaaggcat taagaccaag aagtccgcgg
    17761 ctaaacggtt caagatcacg gcccgcggaa aagtgctgcg cgccagcgcc ggccggcggc
    17821 atttatgcca atccaagagc cccaaacgcc gccgcgcgct gcggggtccg gctgtggttg
    17881 atgcgtcgga cgagtatcgc atcaaacaga acctgccgtt cagccactaa acttaaaccc
    17941 ttagtcattc acgattcatg cgcgtcacca acgctccagc ttcccgcaaa cgccgcgccc
    18001 ggatgattcg agccgccaaa ggcttccgga tgcgccgctc caaactttac cgctacgcct
    18061 cggacgcggt tgatcacggc cgggcctatg cttaccgcga tcggcggacc aagaaacgca
    18121 acttccgcgc gctgtggcaa atccgcatca atgccgccgc ccgcgccgcc ggcacgactt
    18181 acagccgctt catggagggg ctcaaggcgg cccaggtggc gctggatcgc aaaatcctgg
    18241 ccgacctggc agccacggat gccgccgctt ttaaccagtt ggtcaaagtc gcccaggacg
    18301 gtctgaaggc taaagccgcg aaggcttgac atttcaggcg accgattcct cattgccaga
    18361 aagtggacgg gcgacccgaa ccggctcgcc cgtttgcttt tccggcccgc ggcgtcgggg
    18421 agtgcatggc ggagaatgat tttatggctg gattcctgga ccaaatcgaa ccgttaaagc
    18481 aggcggcgct cgccgaactg gacggcgcgg cggacctggc ggcgctggaa caggcgcgcg
    18541 tcaattatct tggcgcgaac ggcaggttca ccgcgctgat gaagcaactg ggcacgctgc
    18601 cccgggagga gaagcccgct gccggcaagg cgattaacgg cgccaaagtc gagttggagg
    18661 ccgcgctggc ccgccgtcgc ggcgaactcg aactcaaggc cgcgctgccc gcggagccga
    18721 ccgacttcac gctgccgggc cgtcggcgca ttctgggaaa gctgcatccg ctgacgcagg
    18781 ttaccgagga catcgtgcgc agcttccgca aaatagggtt tgtcgtggcc gaggggccgg
    18841 aggtggaaga cgattaccac tgtttcgacg cgttgaatac gccggcggat cacccggcgc
    18901 gggatacgca ggacacgttt tacctgggcg gggcggaaac cgaagccgcg ccccccttgc
    18961 tgctgcgcac gcatacgtcc tccgtgcaga ttcgcgtcat ggagaagcaa tcgccgcccg
    19021 tgcgcatcat tgtgccgggc cgctgctacc gccgggataa cgcggacgcc acccacaatc
    19081 ccaccttcca ccaggtggag gggctgtacg tggaccggaa tgtcaccgtg ggggatctca
    19141 agggcacggt cgaatttgtt ttcaaggaac tgatgggcga ggagacgcag attcgcttcc
    19201 ggccgcacta tttcagctac acggagccga gttttgaaat agacttcagc agcccgatga
    19261 cgcgcaagat gggcagggag tggcttgaaa ttgccggctg cgggatggtg catccgcagg
    19321 tgatcgagaa cgttggttat gatgccgagg tttggaacgg gtgggccttt ggctttggca
    19381 tcgagcgcat tgccatgatt cgctacggca tcaacgacat tcggctcttc tacgagaacg
    19441 atctgcggtt cctgaaacag ttttgaccgc catgaaagtc acctttaact ggcttaaaca
    19501 atacgtcgag ttcgattggt cgccggccga actggccgag cggctaacca tgctggggct
    19561 ggaagttgag aacgtccaga agctggaagg cgaattcgag ggcgtggtcg ttgcgcaggt
    19621 ggtcaccaag gacaaacacc cgaatgccga caaactcaat gtctgccggg tcaacgatgg
    19681 ccaaggcgag cgccagattg tctgcggggc gcagaacttc aaggcgggcg acaaggtgcc
    19741 gctgatcctg cccggtttca gcctgcccgc caaacccggc gaggcgccgc tgctcatcaa
    19801 agtgggcaaa atccgcggcg tggaatcgct gggcatgatg tgcgcgccgc aggaactcgg
    19861 cttgggggac gaggtgtcgg ggctgcttat tttacgcgag gacgcccggg ttgggcagcc
    19921 tttttccgag tatctcggcc gctccggcac cgacatggtc tatgacctgg aaattacgcc
    19981 caaccggcct gatctcaata gcgtcatcgg catcgcgcgc gagatcagcg ccgtgaccgg
    20041 caacccgctg cggctgcccg aggtgaatct caccgaagcg agcgggcggg tggcggacgc
    20101 ggtggcggtg cgcattgagg acgccgaatt atgcccgcgg tacaccgcgc gggtgattcg
    20161 cggagtcaag gtcgccgcga gcccggattg gctgcggcac gcactggaaa aggtgggcgt
    20221 ccgcagcatt aataacgtcg tggacgtgac caactatgtg atgctggaga cgggccagcc
    20281 gctgcacgct tttgattatc atttgctggc gaaatcggct gcgaatggcc tgcccaccat
    20341 cgtcattcgc cccgccgcgg agggcgaaaa gttcattacc ctggacggtc aggagcgtgc
    20401 cttgacccgc cagatgctgt tgattgccga cgagaccaag gcggtggccc tggcgggggt
    20461 catgggcggt cagaacagcg aaatcaaccc gaacacggtg gacgtgttga ttgaaagcgc
    20521 ctgctttaag ccgcagaaca tccgcgccac ctccaggaag ctggatttgc gcacggagtc
    20581 gtcgtaccgc tatgaacgcg gcgcggatat cgggatttgt gattgggcca gccagcgggc
    20641 ggcgcagttg atcctggcaa cggcgggcgg caagctggcc caggggctgg tggatgcctg
    20701 gccgcagcca tttgcgcccc ggcagattac cctgcgccat gcccaatgcg atgcggtgct
    20761 cggcgtggcg attccggcgg cgcagcaaac ccagttctta cagcggttgg cgttgaaggt
    20821 tgaggcgggc cccgcgggcg agccgccggc aaccacgttt ggcgtgccgt cgtttcgcgt
    20881 ggacttgaaa cgcgagattg atctgattga ggaagtggcc cggctttatg gggtggataa
    20941 gattcccgcc acccctccgc gcggcgccgt cggcgcccat ccccacgatg cggtgcatga
    21001 tcaactcgcg aatgtccgcc gtattctgac cggactcggc ctgtatgagg cgcaagggca
    21061 gacgctcgtt tcggacgtgg tcgccaggct tactcacggc agtgcgctgg tcccgctgtc
    21121 caacccgctg agcagcgata tgaacgtgct gcggcccagc ctgctgccgg gcttgctcgc
    21181 ggcattgcgc cataatgtta gccgcaaaat caatgacctc gccttgttcg aggtggggcg
    21241 cgtcttcctt gagagcgaag gccagcgtcg tgaagaacgc cgcgtggctc tggccttgac
    21301 cgggcagcgc catccgcttt tctggagcgg agaggagcgg gaggccaggt ttgatatcag
    21361 tgatcttaag ggcttgctgg aggaattcct cgagcaattt ggcgtgcgcg gaatgaatta
    21421 cgtgcgccgc ccagagagca ccgccctctt ccttgagtcg gccaccgtgc atctcggccg
    21481 cttccagctt ggcgagtttg gtctattgct gccatcgctg gctcggcagc atgatttgcg
    21541 ggatgccgtg ctgttggccg agttaaacct tgacctgctg ttggcacgcc gcaacccaaa
    21601 taaatcgttc cggccgctgc cggccttccc ggccattcgc cgggatatag ccatgattct
    21661 gcccgcggcg accacccatg agagcatact gcaagtgatt aagcaggcca aaccggcgaa
    21721 tttggaggcg gtggagctgt ttgacgtgtt ccggggcaag aatgttcctg ccggccaaaa
    21781 gagcatggct tacgcattca cttatcgcca cgccgaccgg acgctcacgg acgccgaagt
    21841 caacgccgtg catgataaac tggtggtcca cctcaagcaa aggttgcagg ccacggtgcg
    21901 ggaatagagg ttcttgacgc gaaacgtgaa tgggggcgag gcccgggaac tggcaccacg
    21961 gcggtaaggc gcccgcccgg tttctccgta tccgttcaag tgctaccgac taagcaagta
    22021 cagggcgcaa gccaggaccc ccgaaaccac cagggctacc gcgagcgacc agcgcaagtg
    22081 gcgcagctct ttgcgccgcc gtgcttttcc gcccatgccc ggcagcaaat aaaatcgctg
    22141 cggctctttg tctcgctcaa accagcgcat cgcaaaaagc ttcatgatcg gaaatggttc
    22201 cctgctgctg aactcttatt cacgcaaatc gccaactcct tccacgagga ttccaggctg
    22261 cctgcccggc ggcctgaaag tcatggtgcg cgaacaattc ctgcatgttg aaccacatgt
    22321 ctggccttta acatgccctc ggatgcgcaa aatcgccagt cctttttcca gtcgcccgcg
    22381 gacttcgcgc cgcgcataag cagcaattcg ggcggcggaa aggcattgaa cgtgaagcag
    22441 gttatgctac aacccgcagc gatgaagatt aatcgccggc agttcatcca ggtcagcagt
    22501 ttatccctgg gcgccgtgag cctggccccg gagtggtccc cgggggcggc ggcttccgga
    22561 cccgtcagct tcattgtccg gccgcccagg atgcatttgg gcctggtcac ctacaatctg
    22621 gcgcaggatt gggatatccc cgccatcatc cggaactgcg aaacggcgca attcgaaggg
    22681 gtggaactcc gcacgagcca cgctcacaag gtagaggtca accttacgcg tgagcagcgc
    22741 caggaggtta aaaagcggtt cgccgattcg aaggtccaac tggttggatt gggcagcacg
    22801 ttcgattacc acacgcccga ccaggccaag ctgcgcaagg atatcgaggc gacgcgcgaa
    22861 taccttgtcc tggcccatga tgtgggcgct cacggaatca aggtgcggcc gaatgcgctg
    22921 cccccggagg ttcccgtggc gaagactttg gcgcaaatcg ggcgcgccct gggagagttg
    22981 ggcgacttcg cccgcgatca cggacaggtg attcgtcttg aagtccatgg cgctggcacc
    23041 tcttttcctc cgcatattaa gaccatcctg gatacggcca atcatcccag cgtcggcgcc
    23101 tgttggaact ccaatccgac ggacctggac ggcgagggat gggaccacaa ctttgacctg
    23161 ctcaaggata agatcttttg cgtacatatg cgcgatctgt ttattgaaga ttaccccttc
    23221 cggaagctgc tcacgcgttt aaatgccatc aacttcaccg gcttctgcct ggcggagatt
    23281 ccggccagcg cggaccccgt acgagtgatg aaatactacc gagccctttg gctggcctac
    23341 caggagctgt tgtagccgct gggacccgtg ggcaaattcg tccggttccc gggcgggacc
    23401 acggcgtcaa cgatttggcc tccgccgatt tccatcttcc gatccggcac actccggtac
    23461 ggccaaagct aaggcactga gagcgggctg cctcggccgg cattgtgggg ttccatccct
    23521 caaatcagag acaacgcacg ctctgggttt ggaatctaaa agcatcttcg gcgtcactgg
    23581 tcgcgcacgg cggccaaccg gctttagtct gaactcccgt ttagacgaga agagttacac
    23641 taccatccgg cagctttgcc gcctgcgcga gggtcagcgg ctcccacgaa agccttgcca
    23701 tcgcggctgc gcgccacgat ttgagatacg ccaatcgaag acaactcatt gagtttatgg
    23761 ccgcgccgga ccagttccgt tcgcaagttc tgcggcaagg ccttctcgat cattaattcg
    23821 tccggagacc attggtggtg aatgcgcggc tgggccagcg cttgctgcgg gtccatctcc
    23881 aaatccagca ggcagaccag ttcgaggagg acttgcgaga tgatccgggg cccgccagcc
    23941 gctccgagag cgatgatggg cttgtcatct ttgagcacca gggtgggggc catgctcgaa
    24001 agcgggcgct ttcggggggc gatggcgttg gcgtggccgc ccaccagccc gaagtagttt
    24061 ctgacgcccg gctgaacgga aaaatcatcc atctggttgt tcatcaccac gcccgtgccg
    24121 gggattacca ccttggagcc gaaactcgta ttgatggtgg ctgtgcaggc tacccagttg
    24181 ccctcctggt ccgcaaccga gaagtgagtg gtgtgctttc ggaatacatc ggtctcccaa
    24241 cccggcggcg agccgtgggc ggtgacgctg gtcgtgcgtt ccaggttgat gcggcgcgcc
    24301 agcgtggccg cataagttcg gtccatcagc ccgcgcggga cattcacaaa gtccgggtca
    24361 cccagccaat atgcgcggtc ggcgaaggcc agcttcatgg cttcagcaat cacatgcaga
    24421 cgggtggctt ctccgagcgt tttcaggttg aacggctcca gcaggttgag catctgggcc
    24481 acatgaatgc cgccggaact gggcggggga aaaccaatca cgctatattc ccggtagcgg
    24541 gagcggagag gctcgcgaag acatacctgg taattccgaa agtcttcagc cgtcaggatg
    24601 cctccatttt cccgcatcca tcgttgcacc gcctgcgcaa aggggcctcg gtagaaccag
    24661 tcgctgcctc ccgccgcgat gctccgatag gtagccgcca ggtctggctg tttaagaatg
    24721 gctcctttgg ccagaggctt cccgttcgcg aagaacactg cgcgggatga ttcaaaccgc
    24781 gccaggtctt tcgccacgcg ctggaggcag cgggcgtagc cggcgttgag cggaaaccct
    24841 ttttccgcga ggtccgccgc cgggagcagg aggtcggcga gcttgcgctt gccatgatgc
    24901 gtgacggcga actcgtaagc tgccagcgcc cccggcactc cgctggccag cgccccaacc
    24961 tggctcaggg tggtatcagc cttgccctcg cggataaaca tgtccggcgt ggccgccgcc
    25021 ggcgccatct cgcgcccgtc aatggccacc agggaaccat cggcccggtg aataagcatg
    25081 aagcagcccc ctccgatgcc ggaattgtcg gtgtccacca cccccaacgt cagcgctacg
    25141 gccacggccg catccaccgc gttgccgcct tctttgagca cgcgcactcc cgcatcggtc
    25201 gccagcggat gaacggaggc gaccattccg tgcgcagctt ccgcaccggt tgcgctgacg
    25261 gcggcgagca gaagggacat catgaaaatt actggaaaca ttgacgcgcc cagcatgcca
    25321 gcctgcgtgt ctcctgtcca atcattccgc catttgggcg gcttctctgg cgtttttgaa
    25381 tggagccttc gggacctcgc gcggccgcca taaacaagga ggcctcctcc ccttcgtggc
    25441 tcccgcaagg cgccttgcat ttacggtgcc ggtttggcag agtcagcccg cgaacggagg
    25501 ccaattctct gccgacggca agcgaagggg ccttttccga gtatgtctga ctcatatatc
    25561 cagaacctga tcgcggagcg catcggcggc cggcaatacg gcaaaggggc ggccggttat
    25621 aagttcgaga agattaagca ggccaggcgc gccgcgatgg ccgcgcatcc ggacggggag
    25681 attctggact ttggcatcgg cgagccggat gagatggctt atccggaggt ggtggacaaa
    25741 ctctgcgcgg aagcgcgcaa gccggagaac cgcggctatg ccgataacgg cggcgtggtt
    25801 ttaaaggaag cggccgcgcg gtacctggag cgtgtttgcg gagtcaaggg aatcaacccg
    25861 gaaaccgaag tcatccactc gatgggcagc aaggcggcgc ttgcgatcct gccggcggcg
    25921 ctgatcaacc ccggcgatta tgcgctgatg accgtgcccg gctatccggt tttcggcacg
    25981 cacgcgcagt atctcggtgg tcgggtctat aacctgccgc tgacggagga caacggcttc
    26041 ctgccggacc tggactcggt gccggcgccg gtgcttcagc aggccaaggt gctcgtgctg
    26101 aattatccca ataatcccac gggcgcgacc gcaacgccgc aattcttcgc gcaggtggtg
    26161 gctttcgccc ggaagcatcg gctggtagtc atccacgatg cggcttacgc ggcgctggtg
    26221 tttgagggca ggccgctgag ttttctggcg acgcccggag cccgggaagt cggcgtggaa
    26281 ctgcactcgg ccagcaagtc ttttaatatg acggggtggc gctgcggttt tgtcgcgggg
    26341 aacgaactgt tggtgcgcgc ctatggcgag gttaaaaaca acacggattc cggccagttc
    26401 ctggccatac aacatgcagc cgcccacggc ttcgaccatc ccgaaatcac ggcgcgaatc
    26461 gccgccaggt attcgcggcg gatggatgac ctggtcaagg tgctgggaac cgccgggttc
    26521 caaccccgca aaccgaaagg ctcgtttttc ctttatgtca aggcacccaa agccgcagtg
    26581 agcccagccg gtgcgcgggt tgaatttcag accgccgaag aagcgtccca gtggctgatc
    26641 acagaaaagc tcatctccac agtaccgtgg gatgacgcgg gcgcttacct gcgcttcagc
    26701 gtgacctttg ccgccaggga ccgcgcggac gaagaccgca ttttccatga aatggcccgg
    26761 cgcctcggcg aaggacaatt cgagttttga gccgcgggaa taagctcctc ggaggcttcc
    26821 ggcattccgg cttgatcggc gggcgttgcc gtttcccgct tgcagttccc gcttgttttt
    26881 tcctgcggga actgctattg aagcgctcac agaaacgcga gcgcaccatg catgcggccc
    26941 tgaagcctaa tccggctctg acttggatca gagacgcggg cggttgccgc gagctttctg
    27001 ttcccggcgg ttgccacgcc cgggggcggg agaccgcccg ccgcgccggg ttccgggcgc
    27061 caactcctga cagccacacc ccatgaagac cgtgactctc tggcgacggc tgaacgtagc
    27121 gttctggctg ctggtggtgc tgttgttggc cagcgtgggc ctggcgctgt gggtgtccgg
    27181 cgcccgcagc gaagccgatc ggcgcagcga gcagttggcg gcggcgcggg cgaccattac
    27241 ctacaaagtc gtgttcatca gcgatacggt gcgggggctt ctgctggctc cgcaagatac
    27301 ggtggagaaa cggcataagc gcgaggcgga aaatgacctg gccgcacaac tgaacttcat
    27361 ccgatcggag tttgccggcc accttgccct gttgcgcgcg gtcagcaacc tgagcgactt
    27421 cacttctcat accctcgcgg attttcatcg gctggtgctg gagaccgcgg agaacgatcc
    27481 cgccggggcc atcgcccttt acaaccggga ttatccagcc atccgcgacg agcgcggcaa
    27541 gctgttcaaa gagctggcca tggaaataca gcatgtgcag gaccaggagt cggcggccag
    27601 ccggacgctg gggtgggcgg ggctggcgcc cctagtcatc atccttttgg cgattcccat
    27661 cgtgggccgc gtgcattccc acaccgtcac ccggccgctg aaccggttgg tcgccgccct
    27721 tgagcggatg cggcagggcg actttaccga acgcctggaa gtccggcgcc gcgatgaatt
    27781 tggcctcctg agcgaggggc ttaatcgcct ggccgacaac ctgtcggagc tcgtggggca
    27841 ggtgcagcgc tccggcaacc aggtttccgg cacggccaca gagattgccg cggcggcccg
    27901 cgagcaacag ggcacctccc gcgaaatcgc caccaccaca gcggaaatcg gcaccacttc
    27961 ccggcggatt tccgccacct cccaggagct ggtgcggacg atgaacgaag tcagccaggt
    28021 agccgggcaa accgccgacc tggccggcag cggccattcg gccatcacgc gcatggaggc
    28081 gaccatgctc cagatcatgg aagcttcggg ctcgatcacc gccaaactgg cggtgctgag
    28141 cgaaaaaacg gccaatatca actccgtcgt caccaccatc accaaggttg ccgatcaaac
    28201 caacctgctc tccctcaacg cggccatcga agccgagaaa gccggccaat acggcctggg
    28261 atttgcggtg gtcgccacgg aaatccgccg cctggccgac cagaccgccg tggccaccta
    28321 cgacatcgag aagatggtca aggaaatgca atcggccgtc accgccggcg tcatgagcac
    28381 ggacaagttt tccgaggaag cgcgccgcgg catcgaggaa atccggaggg tgggcacgca
    28441 tttggcccag attatccatc aagtgcaaac cctgaccccg cggtttcagg cggccaatga
    28501 aggcatgcac gcgcaggcgg ccggcgccca gcagattagc gacaccctga cgcaactgac
    28561 cgaggcggcc cagcaaaccg ccgaatcgtt gcggcaatcc aacctggcca tcgagcagct
    28621 caacggcgcc gcccgcagcc tgcaagccag cgtggcccgg ttcaagctct cgtcctagcc
    28681 cgggcatggt gttcctgctt ttccagttgg gctcgggccg ttatgcgctg gaggccagcc
    28741 gtgtggtcga ggtggtgccc ctgctggaaa tgaagccgct cccccaggcg ccgcgcggag
    28801 tcgcgggcat cttcaattac cgcggccgcc ccgtccccgc ggtggactta tccgcgctca
    28861 cgctgggcca gcccgccagc gaacgcctca gcacccggat tatcatcgtc cattgtccgg
    28921 acgagagcgg aaacgatcat ttgctcgggc tgattgccga gcatgccacc gagctgctcc
    28981 ataaagagcc cagtgctttc atagattccg gggtgaagtt gagccgggcg ccctacctgg
    29041 gcccgatcct catggggtcg cccggcccca ttcagtggat ttacgagcag cgcctgcttc
    29101 cggaagccgt gcgcaacgtc ctgttcgcgg aaaccgtcgt ctcccaactt cccgacggcc
    29161 cggcttgatt tgccgccgga gcgcccggcg gctatgcgga cgggcgctta gggcttcccg
    29221 gccagctcgg cgcaggtggc gtcaaagaat ttcttgcact cggcgatctc gggcatcgag
    29281 ttatcccagt tgtattcgta ctcgacgcag aacgccccgc ggaatccctg ccgcttgagc
    29341 tccgccattt gcgctttcac ctggcccacg ccggtgcccc agggcacatc gtgcatggct
    29401 ttggccgcgc cttccttttt cttcccggcc tcggccgcgg ccgccgtttt gggttcttcc
    29461 ggcacgaggt ccttgaagtg gaggcaaatc acgtggcctt cgagcttctt caggcattcc
    29521 cgcgggtcga gcccggagcg gagccaatgg cccgtatccg cgcaggctcc cagcagcagg
    29581 gagcggcctt tgatggcctc cagcacggtg tccgggttcc agtagcgcga gggcttggga
    29641 tggttatgga tggccacctt gatcgcgtat tccttgcaga gcttctccac cacgtcgagc
    29701 gccgccggct ccggttcggc cactaacgta tcaatgccca tccgcttcgc aaactcgaac
    29761 accccccggc tctccgcttc gtcgggcgga agttgcacca ccccgatatt gatcagtttg
    29821 atccccttcg ccgccgccgc ctttcggatc gccgcggcgt ccgcgtccga gagggtcacg
    29881 gttttgcggc cgctcttgcc gtccaggctc acgctcccgg acaggctcat atgcttcagc
    29941 ccgagcgcgg ccgttttgtc aatgcactca aatatggaga acttgcggaa ggtataggcg
    30001 tgaatcgcca tctgccagcc cagttggtcg gccgccgcgg cagccttggg aacgtcctgc
    30061 cccggcagcg aagcgcacgc caggagcgtt aaagtgaagc atgccagttt gcgcataaca
    30121 gtcattctca ggttcagata aacactggct ccagtgaggc ggtttgttgg ccgccgttca
    30181 agccgaaaat gcgggggcgg cggcggcatc tttttcatgg ctgtccggcg gcggaacagg
    30241 cagaatagca tccgaatagg gcgttgcctg ccggaaccgg atcgtatggc cgccggatcg
    30301 aggccacccg gcggcatcgc gcgccccaac atgaaccaga caaccagaaa gcaaaacgtt
    30361 atgaagcttg gattcgtcac cgccatcctc ccggaactga acctcaacca agtcctcgaa
    30421 ttcgccgccg ccgaacggtt cgcgtgcgtc gaagtcatgt gctggccggt cggcaaggcc
    30481 gaacgccggt ttgccggcgt cacgcatatt gacgtggacg gcttcacccg ggcgcgggcg
    30541 gaagacgtca acgccctttg cgcccgccac ggcgtcagca tttcggccct gggatactac
    30601 cccaacccgc tcgacccgga cccggcggtg gcgcgggtcg ccgtgagcca tttccggaag
    30661 gtcattctcg ccgccgaaat gctcggcctc aaaaacgcca atacgttcgt ggggcgcgac
    30721 tggacccgga cggtggacga aaactggccc cgcttcctga agacctggaa accgctcatt
    30781 gcctttgccg aggaccatgg catcaaggtg ggcatcgaaa actgcccgat gtccttctcc
    30841 cgcgatgagt ggcccggggg caagaacctg gccaccacgc ccgtcatttg gcggcgcatg
    30901 ttcaacgaga tcccttcgcg ctgctttggc ctcaactatg atccctccca ctttgtgctc
    30961 cagcaaatgg acccgctcag cccgctgcgg gagttcaagt ccaggctctt ccatctccac
    31021 gccaaggaca tgatgatccg gcccgagcgc ctcaacgagg tgggcatctt cgctttcccc
    31081 aaagaatggc atacgccgcg catccccggc ctgggtgaca ttcactgggg ccggttcatg
    31141 gcggccctgt acgagattgg ctacgacggg cccgtctgca ttgaggtcga ggacgacacc
    31201 ttcggcaaga cgcttgccgg gcgaaagcag gcgctccgaa tcgcccgcaa cgcgctggaa
    31261 cccttcttct gacttcccgc ggcggacggc gcgaagccgc gcggccggcg gttggccggc
    31321 ccgcgggcgg gcgggcgttt gcgttagcgc gggagaatgt ggccctgccg ttacggcttg
    31381 ccccggccca ggcgcggcgt cgcgcgccgt ttgggcgcgc gatagacgaa gaacatctcg
    31441 tcgcccaccc ggcgcgccga gtgaagctcc agccgcgcgg cccgggccag cgcgccggcg
    31501 ccgcggccat ccgcaatggt cggggcggcg cggccgccga agattcgggg gcagaccgtc
    31561 aggtgcaatt cgtccaccag gccggcgcgg aacagggcgt cggccagttc gccgccgcct
    31621 tcgcacagca ggcgcttgat tccccactgg cggcgcagcc agcgcagggc cgccgggaaa
    31681 ttgatttcct tcgcgccgca tacccggacc tcggccccca gcgcgcgcaa ccgcgtccgc
    31741 cgccgcgccg gggcgcgttg cgtcgtgagc acgagaatcg gggaaaaggt gtgctggaaa
    31801 atgcgggccg ccggattcac gctgccggtg cggctcacga tgatgcggag attgaactcc
    31861 gccaacccgc gccgctgccg caggcggcgg tatttcaagg cccccggccc caggtgaacc
    31921 gcgtttaaat ccgccgtgcg cgcgccggcc atgacggcgt cggcggtcgc gcgcaattca
    31981 tacagatgtt cctgatcccg gcggctgcca aaagaagaca ccacccggtt ggccgtggcg
    32041 atcttgccat ccgcggtcat ggccatgttc ataagcacga aaggccgggg cgcggcgcct
    32101 cggggagtca tccgggtttt attcatagaa tgagcatcgc atcgccataa ctgaagaacc
    32161 ggtagcgctc gcggatggct tccgcgtagg cggccagcat ccgctcgcgc ccccgcgtgc
    32221 tgttcggggc ggcaaaggcg ctgacgagca tgaggagcgt tgagcgcggc aggtgaaaat
    32281 tggtcagcag cgcgtcggcg gcccggaagt cgcgcggcgg gtagatgaac aggcgcgtcc
    32341 ggccgtgtcc ggcggtcacg gcggcccggg gccgggtgcc gccgggagga ggggattgcg
    32401 cggcctgcga ttccagcacg cgcagcgagg tggtgccgac ggcaatcacc cgccgcccgg
    32461 ccgaccgggc ggcattgatg acgcgggcag tcgccgcgct gagttcgtag cgttcctcgt
    32521 gcatccggtg cgcggctggc gtttccgcct tcacgggcgc aaaggtgccc aggcccacat
    32581 gcagcgtgag aaaacaaact tgcgtgccgc gcgcggcgat ttgcgccagc agcgcctcgg
    32641 tgaaatgcaa ccccgccgtg ggagccgcca ccgatccgcc gggccgcgca aagaccgttt
    32701 ggtagcgctc ccgatccgat tccaaacggg aaccattcgg gcgggcgata taggggggca
    32761 ggggaacctc acccagggtg ttcagccgct ccaggacatt gggcgttccc gcgaaacgca
    32821 ggcggcggtg gccttccgca ttggcttcaa tcacaaccgc ctgcacgccg ctggggcggg
    32881 cgcgcgcatc ctgaatttga atccgggttc ccgtccgcgc ccgcctggcc ggccgcagca
    32941 tggcccacca gtcattgacg gcgttttcct ccagaagcag cgcctcgaac tgccctcccg
    33001 taagcgcgtt tacgccacgg aggcgggcgg gaatcacgcg cgaattattg agcaccagcg
    33061 catcccccgg atgcagatat tcaatcaaat ccggaaaacg gcggtgctca aggcgtcccg
    33121 agtcgcgatg cagcaccagc aagcgcgact gatcacgctc cggcgccggc gcctgggcaa
    33181 tcaactccgg cggcaactcg aactcaaaat ccgcagtccg catctcccca gagcattgtg
    33241 ctattacgtc agggtggcaa gatttcactc cattttcata gtcaagccag tgaagggaag
    33301 gtttgctcga actccctgtg agccccgaca tttcctgccg cacgagatta acgataacgc
    33361 ttcatgggca tacggattgg cctaatcagc tctgctggcc aaatcagccc aagcatttta
    33421 ctcaccaacc ccatcccaaa ctgtccaatt attggacact ccacgaaatc aatcatttgg
    33481 cgtttgcctg ccctgccaac ccagcgcgaa tcgcgctttt gcccttttag cccgattttg
    33541 ggctaacata tagcccccaa taggccgtgt acaaggggtg tacaagggat gctcaccggg
    33601 tgtacaagct aaaatcctat gtgcatccgg tgagcatccc ttgtacaccc cttgtacacg
    33661 gctctatggc agccaggaaa taggggaaaa tggcgataat gggagctatg gccgggataa
    33721 actcgacgag gaggcaggcg gttggcgcag gcagggggca gtctagtttt ctcagttttg
    33781 agaaacccgg gcgaagtgcg tttcgggagg cggctaa
//
LOCUS       DAOPQG010000029        33650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1709_length_33650_cov_14.951362, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000029 DAOPQG010000000
VERSION     DAOPQG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 33650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33650
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1709_length_33650_cov_14.951362"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..1338
                     /locus_tag="PKI07_04935"
     CDS             346..1338
                     /locus_tag="PKI07_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50430.1"
                     /translation="MVRRLCLAGVILVSGWLLGTAFAEEFKLADGTVLSGELLAASAS
                     DAGVQIKVGDGKYERVPWANFSQADLKNFAKIKKLEPLVEPFIEISQEEKIKKTEVPI
                     QQPARLERPVPHSLLGALSSSGPGLLMLLLLYAANIYAAYEIAIFRARPVPLVCGVAA
                     VLPFIGPIIFLCLPTAVVPVEQTWDAAPAAAAQAPTAEAVNPMQAEGATHSGALRLAR
                     PGSTETVEPGQFQGEEEKPARPATLTFQRGQYTFNRRFIETKFPGFFGVVRRDADRDM
                     VLVIKSARGEYTGQRISRIAANDLHLMVQRGPASEEVMIPFVEIKEIVLKHKDA"
     gene            1343..1582
                     /locus_tag="PKI07_04940"
     CDS             1343..1582
                     /locus_tag="PKI07_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50431.1"
                     /translation="MAQQFGSFTASELYCPKCRRSQPVRERLLLVLPTGELHDLVCSR
                     CATSLGKRTVSAPAVAPVRARSRAARPAPGRGLLR"
     gene            1820..2785
                     /locus_tag="PKI07_04945"
     CDS             1820..2785
                     /locus_tag="PKI07_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HOC50432.1"
                     /translation="MRIPLLILSVLVPFNFAAQAWNSLGHRTVAELAWRKMDAGERRA
                     ATRLLRQHPHYQQILVAHTPEGVDTNHWAFLTAAVWLDLIRPAKAGQPPKPEFITRYD
                     RYQHGIGYPFVQPADQGRVSLRNFTLPRTNAETMLSNALVTLRDRKASPHDQAVSLCV
                     VLHLFGDLHQPLHAATLVTKGKPKGHGLGSGFLVRTRRGEVITLHSYWDSLPGGDLSY
                     PSILALADKLAAAPELQPDRLPEYAQNRTIPAWVQESFRTAVDLGYAPDRVKWVQASA
                     VKSGKVAEADIPAVPAEYARDAHLAMQRRLALAGLRLAEALKQTW"
     gene            2800..4512
                     /locus_tag="PKI07_04950"
     CDS             2800..4512
                     /locus_tag="PKI07_04950"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOC50433.1"
                     /translation="MQRAAWAVVLLLSLLPLRGAERPNLLLILADDIGFSDLGCCGGE
                     IQTPNLDSLAANGLRFTQFYNTSRCCPTRASLLTGLYPHQAGVGHMMVDRNYPGYRSN
                     LNRECVTIAEVLRAAGYRTYMCGKWHVTNRDGPDDDNSNWPLQRGFEKFYGTIRGYGN
                     FFDPSSLCRQNRFITPENDADYQPAVFYYTDAISDNAVRFLRQHQQESPGQPFFLYLA
                     YTAAHWPMQALEKDIAKYRGKYDGGYEPVRQARQQRLRQLGLLDLKWDCAPTVGDWDA
                     IKDKAWEARCMEVYAAMIDNMDQGVGRVLAALRRSGQLENTVIFYLNDNGACAEDMGR
                     TRLPEPPADELKPMSADTLQTRIRLPMQTRDGRAVKSGRQVMPGPANSYIAYGRNWAN
                     VSNTPFREYKHWVHEGGISTPLIVHWPAGIPAARRGRLEPQVGHIIDLMATCVDLAGA
                     TYPRRWNGQPIQPLAGISLAPAFQGNPLKRATPLFWEHESNRAVRDGRWKLVAKAGQP
                     WELYDMRKDRTEMHDLAGKHPRQVRRLASQWDAWAARAQVLPLGAWKASAPDTHAPPP
                     SARP"
     gene            complement(4574..5908)
                     /locus_tag="PKI07_04955"
     CDS             complement(4574..5908)
                     /locus_tag="PKI07_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC50434.1"
                     /translation="MSERLNRRGFVKSAGALLAGASVGGSAWAAAGRGGPALAVRKCE
                     VLVCGGGPSGVAAAVSAARQGAKTLLVEKSGRLGGMGVTGLVGPLMGHVRSPLVDEVL
                     QRIGGRHPVQERLDLDYAAMLHEAGAEVLLHTWVMETRQRGRRVTGVRALTKQGILEL
                     QAGVVVDATGDGDVAFQAGAAYEMGRPGDRLTQPVSIQYRIGGVDPARALLCASEEEA
                     QKVRVPEGVWEDVVLRGMERGELPPNVGVIRVYLSSYPHERIINATQVNGIDATRVDD
                     LTRAELEGRRQAYQVLDFLRRHAPGYEQAYISAMPAVIGVRESRRIRGVKYLTREDVL
                     AGRRYPDAVVWSADFVVDIHNPAGPGQAENFATHVRPYDIPYGCLVPEKTEGLLLAGR
                     CISGSHDAHASYRVQCIVMAIGAAAGVAAALAARQKVLPRQVAVAQIQQALR"
     gene            complement(5971..7704)
                     /locus_tag="PKI07_04960"
     CDS             complement(5971..7704)
                     /locus_tag="PKI07_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC50435.1"
                     /translation="MTNQNTTYLTARPTAWRGAGFQPAWRAAVAGLLALFSLGLTGSA
                     SAAEVIEADVCIYGGTAGGVVAAVQAARMGRTVVIAEFGNHLGGLTSGGLGATDIGNK
                     RVIGGLAREFYRRVAQHYARDEAWRFETRADYIQHRSGRSRADELEGPDATMWTFEPR
                     VAEELLFQMLRETSAAVYFQQRLASVTRQGTRIREIIMENGKVYRARMFIDATYEGDL
                     MAKAGVSYTVGREANAQYGETLNGVRAETPQHQFTVPVDPYLKPGDPASGRLPFIQAG
                     DGGKPGEGDRSVQAYNFRLCYTQNPANRLPHRPPDKYDPAQYELLARYLEALVAAGQK
                     PQLKAFWNPIWMPNGKTDINNNGGFSTDFIGANYDYPEADYATRAKIWQAHEDYVRGF
                     VYFLATSPRVPADMRAEMQTWGPARDEFLDNGHWPHQLYVREARRMVSDYVMTEHHCQ
                     GRQKAEDAVGMGAYGMDSHNCQRIVKNGRVENEGDVQVWGFPPYPISYRSIVPRAGQC
                     ENLLVPICLSASHIAYGSIRMEPVFMILGQSAATAAALALERGCPVQKVPYEALRARL
                     LADKQILRLGN"
     gene            complement(7745..8236)
                     /locus_tag="PKI07_04965"
     CDS             complement(7745..8236)
                     /locus_tag="PKI07_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50436.1"
                     /translation="MQSHELLREVFQKCSPKLVAAELGLSLSMIYKWAEPGEPGAGSG
                     STNPLDRIDALLRCTQDLRIVQWLCQRAGGFFIRNPKTINPHPSYLIPATNEIVQEFA
                     DLLAVVATAAADNQITSREARDIRARWEELKSVTETFVACCEEGNFRLLREKQAGAAD
                     AQG"
     gene            8832..9683
                     /locus_tag="PKI07_04970"
     CDS             8832..9683
                     /locus_tag="PKI07_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOC50437.1"
                     /translation="MQDIFEAQLALARLGISAGSLDGLMGPQTRAALRAFQLHKENLP
                     ASGELDPATRTRLQLAAPPYTNYTITPDDVARLQPLSSTWLGKSRQTALDYETLLELV
                     AEKSHAHPRLIRQLNPAVDWSRLGAGAVVRLPDIAYPDRATKAAFATISLAGKTLEAF
                     DARSNLLVHFPCSIAQRVEKRPVGELRVVAIAPNPNYTFNPDVFPESAEARGLKTRLI
                     LPPGPNNPVGVAWISLDRPGYGIHGTPSPEQVGRTESHGCFRLANWNADYLLRLVWVG
                     MPVLVEP"
     gene            complement(9744..13049)
                     /locus_tag="PKI07_04975"
     CDS             complement(9744..13049)
                     /locus_tag="PKI07_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOC50438.1"
                     /translation="MTGLLSCLVALASVGLLQLAAFAQPSAPAHIDARMMRQPAVSAT
                     QIAFVYAGDIWLVDKAGGVALRISSPRGEESFPRFSPDGSQLAFTGDYDGNEDIYIVP
                     VAGGLPKRITHHGAPDRMLGWYPDGQSLLYATSMTSPKNRFNQLYRVSAQGGLPVKLP
                     VPYGEFGAISPDGKLLAYVPISVDFRTWKRYRGGMNPDIWLFDLEKLTARNLTQSPAA
                     DSVPMWHGETLYFLSDRDDNKRANLWACDLKHNRFRQVTFFREFDVHFPSIGPEDIVF
                     ENAGRLYLLDLRTEKYHEVEIRVVTDRATLKPRRQNVASQILSATISPGGKRALFEAR
                     GDIFSVPAEHGPVLNLTRTSGIAERYPAWSPDGKWIACFSDRTGEYELTVRRAETAPG
                     TNQLGEQTLTRLGPGYRYFPQWSPDSKRIAFIDKALGLNLFDFEARTNQVISRQLWLH
                     HNQLSEFRVSWSADSRWLAWAQDLDNGNSGIALYDCKSNALHQVTSGFYNDETPVFDP
                     DGKYLFFRTGRTFAPSYSEVDETWIYANTAVLAAVPLRPDVLSPLAPRNDAEDAPAKD
                     KKDDDPKKEPGKDSKKKEEKPKPVEITVAGFEQRVVILPPKAGRFGALAAVPGKLLFH
                     RRPRVGAGEEKKPIAFYDLEKREEKIVVEDADFFELSAGREKLLVRKGGDYAILEPKE
                     GQKLDKKLKTGGFEAVIDPVAEWRQIFADAWRIERDYFYDPGMHGVDWNAMYQRYGRL
                     LDDAVTRWDVNYVLGELIAELNASHTYRGGGDLETGRSVGVGYLGCDFALENGAYRIQ
                     RILRAADWDCEVRSPLLPPAVTNVHEGDYLLAVNGEPLDVREDPWAAFQGLGDKPVLL
                     TVNDQPVLAGAREVLVHTLSSEERLRNLAWIEENRRRVEQLSGGQLGYVYVPDTSQNG
                     QNELVRMWRAQVRKPGLLIDERFNSGGQIPHRFIELLDRPLRNLWAVRGGRYLTTPFV
                     THLGPKAMLINGWSGSGGDCFPFYFKQSGLGLLIGMRTWGGLIGITGSPRLVDGGSVT
                     VPAFAIYSTNSQWIIEGHGVEPDIEVVDDPVAMARGADPQLERAVAELMKQLRKHPPA
                     EIKRPAYPKRPGV"
     gene            13589..13834
                     /locus_tag="PKI07_04980"
     CDS             13589..13834
                     /locus_tag="PKI07_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50439.1"
                     /translation="MEKFEKIVVLDNEVQAELVGSVLEERDIPHLLRSYHDSAYDGIF
                     QTQAGWGHIEAPASFRDKILAVVEDIKRQASSDAKPA"
     gene            14147..15454
                     /locus_tag="PKI07_04985"
     CDS             14147..15454
                     /locus_tag="PKI07_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOC50440.1"
                     /translation="MPMQTRFDLTQADIPKAWYNLLADFPEPLPPPLHPGTRQPIPPE
                     ALEAIFPRNLIQQEMSPERWLEVPEPVRDIYALWRPTPLLRAVRFEKALQTPAHIYYK
                     YEGASPAGSHKVNTSVAQAYFNKAAGTKRLATETGAGQWGAALSMACKLFDLECNVYM
                     VKVSYQHKPYRRMLMHTWGATVHPSPSDQTEFGRKLLAEDPHCPGSLGIAISEAIEDT
                     IKHPHTKYSLGSVLNHVCLHQSVIGQEAIKQMELAGEEPDAIFGCCGGGSNFAGLAFP
                     FIGRKITQKKNYRIVGVEPSACPTLTKGELRYDFGDTAETTPLLMMYTLGHKFMPPAI
                     HAGGLRYHGMAPMISHAVKLGLMEAEAYHQTKVFEMGTLFAQTEGLVPAPESAHAIAA
                     VAEEAIKARKAGRKRVILFNLSGHGLLDLSAYETYQSGKLQDV"
     gene            15504..16388
                     /locus_tag="PKI07_04990"
     CDS             15504..16388
                     /locus_tag="PKI07_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOC50441.1"
                     /translation="MRTQLTKSLGSLLIAGLLTLPVFVQAQPTAHYAPGLEGIKAATL
                     PPPGLWLRDYNWFYYADQVNNRHGRKISAADAEAFVYVNAPRLLWITEGQPLGGYLGL
                     DALLPLKYTWIKDVDQTFGIGDFFFEGTWSWHLQQWDFALAAGIWAPTGDFSKSDPTW
                     AGSGYWGEMLTAGATWYPDQEKRWAFSVLNRYEFNQKQRKTHITPGQAYTVEGGLSYG
                     LSKTVDVGAVGYYQQQVTSDTHGSAKDRDRVAGIGPEINAFFPEITLGISLRYVYEFM
                     AESRLQGHTFALTLTKQL"
     gene            16537..17562
                     /locus_tag="PKI07_04995"
     CDS             16537..17562
                     /locus_tag="PKI07_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC50442.1"
                     /translation="MTDQQWNELLAAIAGKTQAKPPVGFIIDSPWLPNWYGVDIQDYL
                     TSDALWFDANLKAIETFPEVIFLPGFWSEFGMCTEPSAFGAKCIFPRNEFPFADKIIS
                     DVAQIGDLSEPNPATDGLLPFMLNRLKGARARLEDIGHKIRFSVSRGPLNIATFLMGT
                     TEFLMALKTDPGPAHKLLRTITQFLKKWHALQRATFPSIDGIFLLDDIVGFIGEDDFK
                     EFARPCFAELYAGDEAVKFFHNDAACAKSIAYYADLGINLYNPGIFNTLAELRQLSGH
                     RLTILGNIPPRDVLAQGTPAEVQAAVQRLRAETPDMSRLILSCAGGMPPGASTENLKA
                     FIAAARG"
     gene            complement(17559..18518)
                     /locus_tag="PKI07_05000"
     CDS             complement(17559..18518)
                     /locus_tag="PKI07_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOC50443.1"
                     /translation="MIVTTKQLFQVAYGKYAIGAYNINNAEQAMGLFRGCLASQAPFI
                     IQISKGARKYTDKRMLEAIIRAADEIFPEAIFAVHLDHGDEETCYDCIDSGFYSSVMI
                     DASHEPFEQNVAITKRVVDRAHAKGISVEAELGMLGGVEEDIKVEEGNACLTDPAEAE
                     RFVQQTGCDSLACAIGTSHGAFKFKGKQSLHFDVLQKIKDRLPGFPLVMHGSSSVPKT
                     EVDRINAAGGQIAGSMGVDPNEYLPAARLGVAKINIDTDGRLVWTRVHREFFRDKPAE
                     FDFRPPGKVFMEEYSKFIASRNQLLGSAGQLAAVRAALPKSAA"
     gene            complement(18663..20105)
                     /gene="pyk"
                     /locus_tag="PKI07_05005"
     CDS             complement(18663..20105)
                     /gene="pyk"
                     /locus_tag="PKI07_05005"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOC50444.1"
                     /translation="MLLPDHKTKIVATIGPASDSPAMLERLLRAGMNVARLNFSHGDL
                     ASHGERIARIRAAEKVTGRRVAIMADLPGPKMRLGRIEPGPIELRAGESFTLTNEDIV
                     GNRQRVATNFERLPRVVKPGNRLFLNDGLVQLVVEQVQGSEVHCRVAVGGELRSRKGL
                     NLPGIDLGISAFTAHDRDCLEFALRAGVDAISQSFVETAADMEAVRAAASALGKHPFL
                     IAKIERADALRHLDDILKATDGIMVARGDLGVEVPIQEMAVLQKQLIARASLVGKPVI
                     TATQMLESMVVNRLPTRAESTDVANAILDGTDCIMLSGESAMGHYPEEAVAMLAKIAA
                     FTEAHRPPTRLDDLWALSSQRQPATTAEAIVSVVENALGSVPCAAVFVPTWTGTTARM
                     ISRFNPAVWVVAISQSAAVCQGLAFSYGVQAVQLAEDPEDWRAFARQWLREHRLPGAV
                     AMLVAGPSRRHPEANYRIEFLRVNDAPQPA"
     gene            complement(20223..21710)
                     /gene="rhaB"
                     /locus_tag="PKI07_05010"
     CDS             complement(20223..21710)
                     /gene="rhaB"
                     /locus_tag="PKI07_05010"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HOC50445.1"
                     /translation="MPNTYLAIDLGAESGRLVAGLWNGKTIRLEEIHRFPNGPVYLGD
                     SLRWDVVRLWAEIQNGLSLAARKYGPGIVSVGADTWGVDYVLLDERDEMLGQPFAYRD
                     ARTNGMMEKAFRKVPRAKIFAQTGLQFMQFNTLFQLLAHKQGAPKMLERADALLLMPD
                     FIHWALCGSRVVEFTNGTTTQCLHPLTRNWATGLLKQFGLPTRIFPKVVPPGTRLGVL
                     RPGVAERTGLRKINVVAPPTHDTAAAVAGVPTAHTGKANWAYLSSGTWSLMGVEIQKA
                     SLSARTQALNLTNEGGLDGTYRLLKNIMGLWLVQQCKRAFDARGRKYDYGQLARMADR
                     APALRSIVNPDDARFLNPPDMPKAIQAFCRETRQPAPRTEGELVRCAYESLALKYREV
                     LGWLEELTGNRIEVIHIVGGGSQSAILNQFTADACQRPVVAGPVEATAMGNLLVQVRA
                     SGELATLAEMREVIRKSSKVAVFKPGPAAAWEDAAVRFAGLRRGN"
     gene            complement(21778..22887)
                     /locus_tag="PKI07_05015"
     CDS             complement(21778..22887)
                     /locus_tag="PKI07_05015"
                     /inference="COORDINATES: protein motif:HMM:NF018123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOC50446.1"
                     /translation="MMDATQATTNPALGVMIFTLGGLAGAVFYLPFKKVRNWAWESYW
                     FIYAVFGLLVVPWALAMITSPNVLSVLQEAPARTLAYCCLCGAAWGVGGLTWGLMIRY
                     LGVGLGLAIGCGLCSAAGTLVPPILAGKVGSLLGTPSANASMVGVLVSLVGIVLVGMA
                     GMSKEKELPEAEKKKAVAEYNFKKGITVAIFSGLMSSAMSFGLQGGARLEELAEGLMP
                     ATSTTWRGMPVLVVVLFGGFLVNGVWCLYLNARNKTTNDYTRKDAPLLGNFLFAGLAG
                     AIWCSQFICFKTGEPAMGKMAYIGWAVLMASAILFSTLLGIFLGEWKGTSSRTRGLLA
                     LGLVLLLSSTVIAAYSGKLSQEKSASVPPPADSTS"
     gene            complement(22902..24170)
                     /locus_tag="PKI07_05020"
     CDS             complement(22902..24170)
                     /locus_tag="PKI07_05020"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOC50447.1"
                     /translation="MSNTKNIEQSYQLAKERYAQVGVDTDKALKQLAQIPISIHCWQG
                     DDVGGFENAGQELGGGLAVTGNYPGKARTANELRGDFEKALSVIPGRHRFNLHACYAE
                     MSGRKVDRDALSADQFKNWIAWAKALKIGMDFNQTYFAHPKVVGGFTLTSADAGIRKF
                     WIEHGIRCREIGAAIGKALGKTCVTNLWIPDGMKDTPADRRGPRERLIQSLDAVFKKK
                     IDRKLNLDSVESKLFGIGAESYTPGSSEFYLGYAASRQILLTLDAGHYHPTEGIADKI
                     SAVLNYVPEIALHVSRGVRWDSDHVVTLTDELQAIAQEIVWGDYTRRVHIGLDYFDAS
                     INRVAAWVIGTRNMVKALLLALVTPINKLKQFEAEGDYTSRLALMEDAKLLPAGAVWD
                     YYCQQMNVPVGNAWLAEVKRYERDVLSKRQ"
     gene            24347..25102
                     /locus_tag="PKI07_05025"
     CDS             24347..25102
                     /locus_tag="PKI07_05025"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50448.1"
                     /translation="MSALLRATWRVRQKIRAFTLIELLVVIAIIAILAALLLPALAKA
                     QQKALQANCTSNLKQVGLACSLYTQDYNDYLPGPCWSGMFCIYKDNAPNQTIAENPNK
                     YFGALAAYLTTYLASPPPSGTIAYTSKVMVCPAGWKKIPGKQSLNIPSSVPVLYFSPD
                     TIYTDPYNTNQVAFLFPFGRPNTPYAAPRKVTNIPNPAGQWAITDADKYNVPPGATYF
                     NWLPNEPVHGTLRPAMRQTLFFDWHVQSRKTIP"
     gene            complement(25344..25775)
                     /locus_tag="PKI07_05030"
     CDS             complement(25344..25775)
                     /locus_tag="PKI07_05030"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOC50449.1"
                     /translation="MAEVKYLAGKVVRWPLPILTGRPGPDAPTLKRLRLAQGELAQVH
                     DSDEGIHYLATLELREGGVRGNHYHRIKRERVYVLQGRLTVVVEDIQTRERATVPLAT
                     GDLLFIEPGIAHALQTAEAGQALEFAPGRFDPADIYPYPLI"
     gene            25900..27249
                     /locus_tag="PKI07_05035"
     CDS             25900..27249
                     /locus_tag="PKI07_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOC50450.1"
                     /translation="MNGDQRASAVASEMPSRCRRRRLRLCLALGFLAWGGAAARADWP
                     EFRGPRGDGHAVTPGGAPVAGLPLHWNETNNVKWKTPIPHRGWATPVVMGGQVWLTTA
                     TLEGHDFFALGLDAGTGRIRFHEKLFHSDNPEPLGNGASMNCYATPSPVIEPGRVYVH
                     FGSFGTACLDTRSGQTLWKRDDLPCRHYRGASSSPVLFENLLILTFDGANLQYLAALD
                     KQTGRTVWKTDRSVAWNDEHEPGQMAREGDHRKAHGTPLIVTVAGKPLLLSAGAKAAY
                     GYDPRTGRELWKVRYNDYSTAPRPVFDRGLAFFVTGLATKELWAVKTDGRGDITDTGV
                     AWKFNRRVGIYASPLLVDGLLYTAAAENYLTCVNAATGESVWSERIPGKYAASPIYAD
                     GRIYLFNQSGVTTVVKPGRAFEVLATNNLADGFMASPAVSGKAFYLRTKTHLYRIEQ"
     gene            complement(27295..28863)
                     /locus_tag="PKI07_05040"
     CDS             complement(27295..28863)
                     /locus_tag="PKI07_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50451.1"
                     /translation="MNKHPLSRGDAPRPGRTQSTAVSRREWLQGMLSAGSVLALGGCA
                     LPRSSSPGSSAGLIRRENQRPGTRDWLLTKTGIDPATQYRCPWIEGYCSHTSIRAGQT
                     LTFHVSTNPPATFTLDLYRLGYYGGAGGRLMKRLGPFQGAQQPDPPIGPRRLRDCQWE
                     ACAALKIPADWLSGVYVGKLTAQPAGWQSYVIFIVRDDRRADFLFQCSDTTWQAYNRW
                     PNQFSLYDDGLARWYWGNDVAVSFNRPYGKYCQILDAPLSTGSGEFFLWEFPLAYWLE
                     EQGYDVSYISNLDTHADPAGLLRARGFLSVGHDEYYSLEMFRHLQHAIRQGLNVAFLS
                     GNTCCGRIEFQPDFRGRLRTYERVDFYGPRDPKPIRGMEKLPHTSPSSAELVGARNLG
                     PFSGGADWICLKPEHWLFAGTGMKRGEGIPGLVGWEWHGDPAAIPGLEIVAGGPTQWK
                     PGVLNHGHYTATIYPGPRGNFVFNASTCWWGDGLSEPPGYVRPSVYTQPAGPDSRVRQ
                     ITRNLLEQMRRPAR"
     gene            complement(28962..30356)
                     /locus_tag="PKI07_05045"
     CDS             complement(28962..30356)
                     /locus_tag="PKI07_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50452.1"
                     /translation="MPKTNSPGLTRRAFLLRAGAASLLAAPVIIPASVRGRNGAVAPS
                     ERIQVGLIGLGAMGRGHLRILAGHSQTQLVAVCDVDRVRREEGAQRVDEISAARREAG
                     SFHGCAAIHDYRELLARSDIDGVVIATPDHWHALQAIHAAQAGKHVYCEKPVSLTIRE
                     GREMVETVRRYARVFQTGTQYRSIPSIRQVCQFVRGGGLGKVKAAFTLWMKIEIPTLG
                     GSYIPLDPALPAEPVPAGLDWDLWVGPAAWHDYNAAYHRNPVPGVVPWTFCRAFGAGP
                     ITNYHSHAADVMQYALGVETGGPVEILHPASGAFPTLTCRYANGVLLHHVDHWGQVKE
                     LYRAVPAEARLEGLFGGLFVGERGWITSMSGAGPIEGGPEGILREAGLTTRKVNIGAN
                     NHHANWFDCLRSAGRPSAHEEIGHRSASLGHLVALAYQLGRSLRWDPAREVFPDDAEA
                     NRLLARARREPWLA"
     gene            complement(30364..32148)
                     /locus_tag="PKI07_05050"
     CDS             complement(30364..32148)
                     /locus_tag="PKI07_05050"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOC50453.1"
                     /translation="MRTILIRMPHTCPDDCSLAVLPDARVKPLTRRAFLARAAAQALA
                     LGCGPAVLRAASGPGAGAERELIEAALPARAPARPARPRKLLIFDRNVNYGGHGSIPA
                     ANLAFELMGRRTGAFETVVSRDPEVFRPERLRQFDGVFFNNCVGNLFTDAALRQSLVE
                     FVYGGGGLMGVHGSTVAFTQWPGAVEDWPEFGLMIGARGANHRASDEQVFIKLEEPEH
                     PLNQALGGRDFAYRDEFFRVQDPYSRRRVRVLLRIDTDKTDLAGRPPERADHDYALAW
                     VRHYGRGRTFYCTIAHHPAVFRDPKLLSFYLAAAQFILGDLPAPTVPSAWLTPALRAQ
                     EKLGWRLGIEAYTFHKFTLFEAIEKTAALGLPFMGGLSFQKVGGGIARNFEPGLSDEE
                     LRQIRFKLDAAGVRLLTYYLQEIPGDEAGCRKVFEFGRQIGIETFMSEPKPESLDMIE
                     KFCDAYDLKVALHNHDPQASPAYWNPAGILKACAGRSQRLGACADLGYWMRAGIDPLA
                     GVRQLKDRLLTLQMHDLHARGADGHDVPWGAGVGNTAALLEEIHRLGLQPTMFGLEYS
                     HNWLASMPDIARCIEFFNRVSLDLARRK"
     gene            32532..33218
                     /locus_tag="PKI07_05055"
     CDS             32532..33218
                     /locus_tag="PKI07_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC50454.1"
                     /translation="MAQAKRKQVMDSRKRILLVDDHAVVRFGIAQLVNQQSDLVVCGE
                     EEDAANALRAISQLKPDLVIADISLKDSSGLELMRNIKAQHAGLPVLVVSAHDESLYA
                     EVAFRAGALGYLMKADALEKVLVAIRRVLDGNVYVSDALAARLLQQQVRGARCIQESP
                     VKGLSDRELEVFQLIGQWKKTREIAQALHLSIKTIEYYREQIKQKLDLKGTAELTQYA
                     TAWVQRENPA"
BASE COUNT         6180 a        11213 c        10377 g         5880 t
ORIGIN      
        1 gttgtttgat tgccaggccg cacgggccgc ccggcggccc gtgccttacg agcctgtcgt
       61 ccggattgcc atgctgcctc agccacggat caaaggctac cgtatgggcg gacgaggtca
      121 acgcgagagt ggttttcgag acggaaatgg gcgcaacgca tgtttttcca aggcgcagcc
      181 cttccggctg gcaagcgttt gcctcaccgc ccaaagttgg cgaagacacc ggagctccga
      241 ttacgccgaa tgaacggatg ggaaagaggc gggccgcgtc tgccggggcc gggattctgg
      301 atttttgctt tccttggggc gggaagctct gttaagaaag cgctcatggt gcgcagatta
      361 tgtttggccg gagtcatcct ggttagcgga tggctgctcg ggaccgcttt cgccgaagag
      421 ttcaaactcg ccgatggcac ggtgctttcc ggggagctcc tggcggcgtc cgccagtgac
      481 gcgggggtgc aaatcaaggt gggcgatggc aaatatgagc gggtgccctg ggcgaatttc
      541 tcgcaggcgg atctgaagaa ctttgccaaa atcaagaaac tggagcccct ggtggagccg
      601 tttatcgaga tctcacagga ggaaaagatc aagaagaccg aggttcccat ccagcagccc
      661 gcgcggttgg aacggcccgt gccgcactcg ctgctgggcg cgctttcttc ctccgggccg
      721 gggctgttga tgctgctatt gctttacgcg gccaatatct acgccgctta cgaaatcgcg
      781 atcttccgcg cgcggcccgt gccgctggtt tgcggcgtgg cggcggtgct gcccttcatc
      841 gggccgatta tattcctgtg cctgcccacg gcggtggtgc cggttgagca gacctgggac
      901 gccgcgccag ccgccgcggc ccaggcgccg acggcggagg cggtcaaccc gatgcaggcg
      961 gaaggggcga ctcattccgg agcgttgcgg ctggcgcggc ccggctcgac ggagacagtg
     1021 gagccggggc aattccaggg cgaagaggaa aagccggccc ggccggcgac gctcaccttt
     1081 cagcgcgggc aatatacttt caaccggcgt ttcatcgaga ccaagtttcc cggcttcttc
     1141 ggcgtggtgc gccgggatgc cgatcgggac atggtgctgg tgatcaaatc ggcgcgcggc
     1201 gaatatacgg gccagcggat cagccgcatt gccgccaacg acctccacct gatggtgcaa
     1261 cgaggccccg cctcggagga agtcatgatc ccgttcgtgg agatcaagga aatcgtgctc
     1321 aagcataaag acgcgtagaa cgatggcgca gcagttcggt tccttcaccg ccagcgagct
     1381 ttattgtccc aagtgccgcc gctcccaacc ggtccgcgag cggctgttgc tggtgctgcc
     1441 gacgggagaa ttgcatgacc tggtctgctc ccgctgcgcc acatcgctgg gcaaacgcac
     1501 ggtgagcgcg ccggcggtgg cgcccgtccg cgctcgttcc cgcgcggccc ggcccgctcc
     1561 cgggcgggga ttgctgcgtt aaccgggccc ggcgtcccgg gcgccggaat gggttttcgg
     1621 agccggtttt gggggaaaaa tcccgcgccc ggcccggcgg cggaacgggc cagccaggcg
     1681 tttgcccgga ttcgcgctcg tggccggccg aggcgctcgg gctccctgct ccgtcccctg
     1741 cccttttcct tgcgcgccgc agccggtgtt tattttggct ggtcttgcgc cgggcgccgc
     1801 cccatgatag ccggtgatta tgcgtatacc actgttaatc ctgagcgttc tggttccgtt
     1861 caactttgcc gcgcaagcct ggaactccct gggacaccgg acggtggccg agctggcgtg
     1921 gcgaaagatg gatgccggcg aacgccgggc cgccacccgg cttttgcgcc agcatccgca
     1981 ttaccagcaa atcctcgtcg cccatacgcc ggagggggtg gacaccaatc actgggcgtt
     2041 cctgaccgcc gcggtgtggc tggacctgat ccgtccggcc aaggcagggc aaccgccgaa
     2101 gccggaattc atcacccggt acgaccgcta tcagcatggc atcggttatc cattcgtgca
     2161 gcccgccgac caggggcgcg tctccttgcg caatttcacc ctgccccgga ccaatgcgga
     2221 aacgatgtta tccaacgcgc tggtcaccct gcgcgatcgc aaagccagcc cgcacgacca
     2281 ggcggtgagc ctgtgcgtgg tgctgcacct gtttggcgat ttgcatcagc cgctgcacgc
     2341 cgccaccctg gtcaccaaag gcaaacccaa aggccatggg ttgggcagcg gctttttagt
     2401 gcgcacccgc cgcggcgagg tgatcaccct ccactcttac tgggactctt tgcccggcgg
     2461 ggatctctcc taccccagca ttcttgcgct ggccgataaa ctcgccgccg cgcccgaact
     2521 ccagccggac cggctgccgg agtatgcgca gaaccgaacc atccccgcct gggtgcagga
     2581 gtctttccgc acggcggtgg atctcggcta cgccccggat cgcgtgaaat gggtgcaggc
     2641 gtcggcggtc aagtccggca aagtggccga ggccgacatc ccggcggtgc cggcggaata
     2701 cgcgcgcgac gcgcacctgg ccatgcagcg gcgcctggcc ctggccggat tgcgcctggc
     2761 ggaggccctg aagcaaacct ggtaaccggg agcttcctta tgcaacgcgc ggcctgggcg
     2821 gtcgttttgc tgctgagcct cctgcccctg cggggggcgg agcggcccaa tcttctgctg
     2881 atcctggccg acgacatcgg cttttccgac ctgggctgtt gcggcgggga gattcaaacg
     2941 ccgaacctgg attcgctggc ggccaacggc ctccggttca cccagttcta caacacctcg
     3001 cgctgctgcc ccacgcgggc gtctttgctg accggcctgt atccgcacca ggcgggcgtc
     3061 ggccacatga tggtggaccg caattacccg ggttaccgca gcaacctgaa tcgcgagtgc
     3121 gtgacgatcg cggaagtatt gcgcgccgcc ggctaccgca cgtatatgtg cggcaaatgg
     3181 cacgtcacga accgcgacgg ccccgacgac gacaactcca actggcccct gcagcgcggt
     3241 tttgagaagt tctatggcac catccgcggg tatggcaatt tcttcgaccc gtcctccctg
     3301 tgccggcaga accggtttat cacccccgaa aacgacgccg attaccagcc cgcggtcttt
     3361 tattatacgg atgcgatcag cgataacgcg gtgcgcttcc tgcggcagca ccagcaggaa
     3421 tcgccgggcc agcctttctt tctgtacctg gcctacaccg ccgcccactg gcccatgcag
     3481 gcgctggaaa aggacattgc caaataccgc ggcaagtatg atggcggcta cgaaccggtg
     3541 cgccaggcgc ggcagcagcg cctccggcag ctcggcctgc tggatttgaa gtgggattgc
     3601 gcgccgaccg tcggcgactg ggacgccata aaagacaaag cctgggaagc gcggtgcatg
     3661 gaggtttacg cggcgatgat tgacaacatg gaccagggcg tcgggcgcgt cctggccgcg
     3721 ctccggcgct ccggccagct tgagaacacg gtcatcttct acctcaacga caacggcgcc
     3781 tgcgccgagg acatggggcg cacccggctc ccggagccgc ccgccgacga gctaaagccg
     3841 atgagcgcgg acacgcttca gacgcgcatt cggctgccga tgcaaacccg cgacggccgg
     3901 gccgtgaagt ccgggcgcca ggtgatgccc ggcccggcca acagctatat cgcctatggc
     3961 cgcaactggg ccaatgtctc caacactccc ttccgcgaat acaagcattg ggtgcatgag
     4021 ggcggcatca gcacgccatt gatcgtgcat tggccggcgg gcatccccgc cgcgcgccgg
     4081 ggccggctgg agccgcaggt cggccatatc attgacctga tggccacctg cgtggacctg
     4141 gccggggcca cctacccgcg ccggtggaac ggccagccga tccagccgct ggcgggcatc
     4201 tcgctggcgc cggcgttcca gggcaacccg ctcaagcgcg ccacgcccct tttctgggag
     4261 catgaaagca atcgcgccgt ccgcgacggg cgctggaagc tggtcgccaa agccggccag
     4321 ccctgggaac tttacgacat gcggaaagac cgcacggaaa tgcatgacct ggccgggaag
     4381 cacccgcggc aggtgcggcg gctcgccagc cagtgggacg cctgggccgc ccgcgcccag
     4441 gtgctgcccc tgggcgcctg gaaagcgagc gcccccgata cccacgcgcc gccgccgtcc
     4501 gcgcgcccct gaccgggttg aggccgcgcc cgcgaagccg tggcggcctc cggcccgccg
     4561 ccgggcatga gcctcagcgc agcgcctgct ggatttgcgc gacggcgacc tggcgcggca
     4621 gaactttctg gcgggcggcc agcgcggccg ccacgcccgc ggcggcgcca atggccatca
     4681 caatgcactg cacccggtag gatgcgtggg cgtcgtggct gccactgatg caccgcccgg
     4741 ccagcagcag cccctccgtt ttttccggca ccaggcagcc ataggggatg tcataaggcc
     4801 gcacatgggt ggcgaagttc tcggcctggc cggggccggc cgggttatgg atgtccacta
     4861 cgaaatcggc ggaccacacc acggcatccg gatagcgccg gccggcgagg acatcctccc
     4921 gggtcaggta tttcacgccc cggatacggc ggctctcacg cactccgatc accgcgggca
     4981 tggccgagat ataggcctgt tcatagccgg gcgcgtgccg gcggagaaaa tccagcacct
     5041 ggtacgcctg ccggcgccct tcgagttccg cgcgagtcaa atcgtccacg cgcgtcgcgt
     5101 caatgccatt gacctgggtg gcgttgataa tccgttcatg gggataggag gaaaggtaaa
     5161 cccggatgac gcccacattg ggcggcaatt cgccccgctc catgccccgg agaaccacgt
     5221 cctcccacac gccttccggc acacgaacct tctgcgcctc ctcctcgctg gcgcagagca
     5281 gtgcccgcgc cggatccacg ccgccgatgc ggtattggat tgaaaccggc tgcgtcaacc
     5341 ggtcgcccgg acgccccatc tcgtatgccg cgccggcctg gaaggccacg tcgccatcgc
     5401 cggtggcatc caccaccacg ccggcctgca attcgaggat gccctgcttg gtcagggcgc
     5461 gcacccccgt cacacgccgg ccgcgctggc gcgtctccat cacccaggta tgcagcagga
     5521 cctcagcgcc ggcttcgtgg agcatcgccg cataatccaa atccaggcgc tcctgcaccg
     5581 ggtgacgccc gccaatgcgc tggagcacct cgtccaccag gggactccgc acatggccca
     5641 ttaacgggcc aaccaggccg gtgacgccca tgccgcccag gcgcccgctc ttctcgacca
     5701 gcagcgtctt cgcgccctgg cgcgcggcgc tcaccgccgc cgccaccccg gacggaccgc
     5761 cgccgcacac caacacctcg catttcctga ccgccaaggc ggggccgccc cggcccgccg
     5821 ccgcccacgc cgaaccgccc accgacgcgc cggccagcaa ggcgccggcg gatttcacga
     5881 agccgcgccg atttaatcgc tcgctcatga cgcaagtctc gcgaggcggg cgggtctagt
     5941 caagccagcc gatccgggcg tccgcccggg ctagttcccc aatcgcagga tttgtttatc
     6001 ggccagcaaa cgggcgcgga gcgcctcata cggaactttc tgcaccgggc agccgcgctc
     6061 aagggccagg gccgcggcgg tggccgccga ctggccaaga atcatgaaga ccggttccat
     6121 gcggatgctg ccgtaggcga tgtgtgacgc cgaaaggcaa atggggacca gcaggttttc
     6181 acactggccg gcccggggca ctatcgagcg gtacgaaatc gggtacggcg ggaagcccca
     6241 tacctgcacg tcgccctcgt tttccacccg cccgtttttc acgatgcgct ggcagttatg
     6301 cgagtccatg ccataggcgc ccatgcccac ggcgtcttcc gccttctgcc gcccctggca
     6361 atggtgctcg gtcatcacat agtccgacac catgcgccgc gcctcgcgca cgtaaagctg
     6421 atgcggccag tggccgttgt cgaggaactc gtcccgcgcc ggcccccagg tctgcatctc
     6481 ggcgcgcata tccgcgggca cccgcggact ggtggccagg aaataaacga agccgcgcac
     6541 gtagtcctcg tgcgcctgcc agattttggc gcgggtggca tagtccgcct ccgggtagtc
     6601 atagttggcg ccgatgaagt cggtggaaaa accgccgttg ttattgatat cggtcttgcc
     6661 gttgggcatc cagatggggt tccagaacgc cttgagctgg ggtttctgtc cggcggccac
     6721 gagcgcttcg agatagcggg ccagcagttc atattgggcc gggtcgtact tgtccggcgg
     6781 acgatgcggc aggcggttgg ccggattctg cgtgtagcaa agacggaagt tataggcctg
     6841 gacactgcgg tccccctccc ccggcttgcc gccgtcgccg gcctgaatga atggcaaccg
     6901 cccgctggcc gggtcgccgg gcttcaggta cggatccacc gggacggtga actggtgttg
     6961 gggggtttcc gcccgaaccc cgttgagcgt ttctccgtat tgggcattgg cttcccgccc
     7021 caccgtgtag ctgaccccgg ccttggccat caggtcgcct tcatacgtgg cgtcaataaa
     7081 catgcgggcg cggtaaacct tgccattctc catgatgatc tcccgaatgc gcgtgccctg
     7141 ccgggtgacg gaagccaggc gttgctgaaa atagaccgcc gcgctggttt cgcggagcat
     7201 ctggaacagg agctcttccg ccacgcgcgg ctcgaaggtc cacatggtgg cgtccggccc
     7261 ttcgagttcg tccgcccggg aacggccaga ccggtgctgg atgtaatcgg cgcgtgtctc
     7321 gaaccgccac gcctcatccc gcgcgtaatg ctgcgccacg cgccggtaaa actcgcgggc
     7381 caggccgccg atgacccgtt tgttgccgat gtcggtcgcg cccagtccgc ctgaggtcag
     7441 gccgcccagg tgattgccaa actcggcaat gactactgtc cgccccatgc gcgccgcctg
     7501 gacggcggcc accaccccgc cggcggtccc gccatagatg cacacgtcgg cttcaatgac
     7561 ttccgccgcc gaagccgatc ccgtcaggcc caaagaaaac agcgccagca ggcccgcaac
     7621 cgcggcgcgc caggcgggtt gaaatccggc cccgcgccag gccgtgggcc gagccgttag
     7681 ataggtagta ttctggttcg tcataaagct ggtatttctc gaatacggtc gccgtctgcg
     7741 gacgtcagcc ctgggcatcg gccgctcctg cctgtttttc gcgcagcaac ctgaaatttc
     7801 cctcctcgca acaggcgacg aaggtctcgg tcaccgattt gagttcttcc caccgcgccc
     7861 ggatgtcccg cgcttcccgg gacgtaatct ggttgtcagc ggcggcggtg gccaccaccg
     7921 ccagcaaatc cgcaaactcc tgcacgatct cgttggtggc ggggatcagg taggagggat
     7981 gcggattgat ggttttgggg ttgcgaataa agaaaccgcc cgcccgctgg caaagccact
     8041 gcacgatgcg caggtcctgg gtgcaccgca gcaacgcgtc aatccgatcc agcggattgg
     8101 tgctgccgct gcccgcgccc ggctcgcccg gctcggccca tttgtaaatc atcgaaagcg
     8161 acagtcccaa ctcggcggcc acgagtttcg ggctgcactt ctgaaagacc tcgcgcaaga
     8221 gctcgtgcga ttgcatgcgg ccagcatgcg tgactcccgg cccgttcgca aggaggaagc
     8281 gcgcgtttgc gctcgaagaa aaccggcttt tgccgctccg aacgcatctc cccgggcgcc
     8341 ccttatgctc ccggctatcc cggcaacgcg gcatgccaat gcgggctggt tccagtttgt
     8401 gcttgggcag gcggcgttcg ttatggctaa atcccgttgt gcggcgcagc accaaatacc
     8461 ggcggtcgcg gtcaaaaggg aaaccgcggc gcggattttt atggctgctg atactggccg
     8521 gactcgcaat cgcagccggc tggatgtggt ggcaggcgcg ccccggccgc ccgcccgcgc
     8581 cccggcccag gcccgccccc gtggccgtga cgtccccggc cgccgttcaa tcccaatcaa
     8641 cccaggccgt ggttcagccc aaaccggcgc cggtggcggt ccctttcccc gcagcaccga
     8701 cacaggcgcc gcctcccgcg ccggtgccgc caccgccccc cgctccgccg ccggtggaaa
     8761 cggctcccgc acccccggtg attcccccgc ctccgttgga gccagcgacg cacgcggcct
     8821 tcccccgccc ggtgcaagac atatttgagg cccagctcgc gttggcgcgc ctgggcattt
     8881 ccgccggttc gctggacggc ctcatggggc cgcaaacgcg cgccgcgctg cgcgcctttc
     8941 agcttcacaa ggagaacctt cccgcctcgg gtgaactgga cccggccacc cggacgcgac
     9001 tgcaactcgc cgccccaccc tataccaact ataccatcac ccccgatgat gtggcgcggc
     9061 tgcagccgtt aagcagcacc tggctcggca aatcccggca aacggcgctc gactacgaga
     9121 cgctcctgga attggtcgcc gaaaagagcc acgcccatcc ccggctcatc cggcagctca
     9181 acccggcggt tgactggagc cgcctgggcg cgggcgcggt ggtgcggctg ccggatattg
     9241 cctatcctga ccgggccacg aaggccgcct ttgccacgat cagcctggcc ggtaaaaccc
     9301 tcgaagcctt tgacgcccgc tccaacctgc tcgtgcattt cccgtgcagc atcgcgcagc
     9361 gcgtcgaaaa gcggccggtc ggggagttgc gcgtcgtggc catagccccc aacccgaact
     9421 acaccttcaa cccggacgtg ttcccggaat ccgccgaagc gcgcgggctg aagactcggc
     9481 tcatcctgcc gcccggcccc aataacccgg tcggggtggc ctggatcagc ctggaccgcc
     9541 cgggctacgg catccacggc actcccagtc cggagcaagt gggccggacc gaatcgcacg
     9601 gctgcttccg gctggcaaac tggaatgccg attacctcct ccggctggtt tgggtcggaa
     9661 tgccggtgtt ggtggagccc tgacgccgcc gggtgcgccg ggcggaagcg cgggtggttt
     9721 tcgcccggct tgccgctgcg gaattagacg cccggacgct tcggatacgc cgggcgctta
     9781 atctcagccg gaggatgttt gcgcaactgc ttcatcaact ccgccacggc gcgttcgagc
     9841 tggggatccg cgccgcgcgc catggcgacg gggtcgtcca cgacctcgat atccggctcc
     9901 actccgtggc cttcgatgat ccattggcta ttggtgctgt aaatggcaaa ggccggcacg
     9961 gttacgctcc cgccgtccac cagccgcggc gagccggtga tgccgatgag cccgccccag
    10021 gtccgcatgc cgatcaacaa tcccagcccg gattgtttga agtagaacgg aaagcagtcg
    10081 ccgcccgagc cgctccagcc gttgatgagc atcgccttgg ggcccaggtg cgtgacgaac
    10141 ggcgtggtca ggtaccggcc gccgcgcacg gcccagagat tgcgcagcgg ccgatcgagc
    10201 aattcgatga agcggtgggg aatctggccg ccgctgttga aacgctcgtc aatgagcagg
    10261 ccgggtttcc ggacctgcgc gcgccacatg cgcacgagtt cgttttggcc attctggctg
    10321 gtatcgggca catagacgta gccgagctgt ccgccgctga gttgctcgac gcggcgccgg
    10381 ttctcctcga tccaggccag gttgcgcaac cgttcctcgc tgctcaaggt gtgaaccagg
    10441 acctcgcggg cgccggccag gaccggctgg tcgttaaccg tcagcaatac cggcttgtcc
    10501 cccagcccct gaaaagccgc ccaggggtcc tcccgaacat ccagcggctc gccgttgacg
    10561 gcgagcaggt agtcgccctc atgcacgttg gtgaccgcgg gcggcagcaa cggcgagcgc
    10621 acttcacagt cccagtccgc cgcccggagg atgcgctgga tgcgataggc gccgttttcg
    10681 agcgcgaaat cgcaccccag gtagcccacg cccaccgagc ggccggtctc gaggtcgccg
    10741 ccgccgcgat acgtatgcga cgcgttcagc tctgcgataa gttctccgag cacgtagttg
    10801 acatcccagc gcgtgacggc gtcgtcgagc aaccgcccat agcgctggta catggcgttc
    10861 caatccaccc catgcatgcc cgggtcgtaa aagtaatcgc gctcgatgcg ccaggcgtcg
    10921 gcgaagattt gccgccactc agcgaccgga tcaatcaccg cctcgaaccc gcccgtcttg
    10981 agcttcttat ccagcttctg cccctccttg ggctcgagga tggcgtagtc gccgcctttg
    11041 cgcaccagca gcttctcccg cccggcggac agctcgaaga aatccgcatc ctcgaccacg
    11101 atcttctctt cgcgcttctc caagtcgtag aaggcgatgg gcttcttttc ctcgcccgcg
    11161 cccacgcgcg gacggcggtg aaagagcagc ttgccgggga ccgctgcaag ggcgccaaag
    11221 cgcccggcct tgggcggcag aatcaccaca cgctgctcaa agcccgccac cgtaatttcc
    11281 accggcttgg gcttctcctc cttcttcttg ctgtccttgc ctggctcctt cttcgggtca
    11341 tcgtccttct tgtctttggc cggggcgtcc tcggcgtcgt tgcgcggcgc gagcggagag
    11401 agcacatccg gacgcagcgg caccgcggcc agcacggccg tattggcata aatccaagtc
    11461 tcatccacct cgctgtagct gggggcgaaa gtgcggcccg tgcggaagaa caggtatttg
    11521 ccgtcgggat cgaagaccgg cgtttcgtca ttatagaacc ccgaggtgac ctgatgcagc
    11581 gcgttggatt tgcaatcgta aagggcgatg cccgagttgc cgttgtccag gtcctgcgcc
    11641 caagccagcc agcggctgtc cgccgaccaa ctcactcgga actcgcttaa ctggttgtgg
    11701 tgcagccata gctggcggct gatcacctgg ttcgtccggg cctcgaagtc aaacaggttc
    11761 aggcccaacg ccttatctat gaaggcgatg cgcttgctgt cgggggacca ttgcggaaag
    11821 tagcgatagc ccgggcccag ccgcgtgagg gtttgctcgc caagctggtt ggtgccgggg
    11881 gcggtttccg cgcggcgcac cgtcagctca tattcaccgg tgcggtcgct aaaacaggcg
    11941 atccacttgc catccggcga ccacgcggga taccgctcgg cgatgcccga agtgcgggtg
    12001 aggttcagca ccggcccgtg ctcggccggg accgagaaaa tatccccgcg cgcctcgaat
    12061 agcgcccgct tgccgcccgg cgaaatcgtc gcgctcaaga tctggctggc aacattttgg
    12121 cggcgcggct tcagcgtcgc ccgatccgtg accacgcgga tttccacttc gtggtacttc
    12181 tccgtccgca aatccagcag gtaaagccgg cccgcgtttt caaacacaat gtcctccggc
    12241 ccaatgctgg ggaaatgcac gtcgaactcc cgaaagaagg tcacctgccg gaagcggtta
    12301 tgcttgaggt cgcaggccca gaggttggcg cgcttgttgt cgtcgcgatc ggacaaaaag
    12361 tacagcgtct cgccgtgcca catggggacg gagtccgcgg cgggcgactg cgtcaggtta
    12421 cgggcagtga gtttctccag gtcaaacagc cagatatccg ggttcatgcc accgcgatag
    12481 cgtttccacg tgcggaaatc cacgctgatc ggcacgtagg ccagtagctt gccatcaggc
    12541 gaaatcgccc cgaactcgcc atacggcacc ggcagtttta ccggcaaccc tccctgcgcc
    12601 gacacgcggt agagctggtt aaaccggttc ttgggactag tcatcgaggt cgcataaaga
    12661 aggctctggc catccggata ccaccccaac attcgatccg gcgcgccatg atgcgtaatc
    12721 cgtttgggca gcccccccgc caccggcacg atgtagatat cctcgttgcc gtcgtagtcg
    12781 cccgtaaatg cgagttgcga tccatccggc gagaagcgcg gaaaagattc ctcaccccgg
    12841 ggcgaactaa tccgcagtgc caccccgccc gccttgtcca ccagccagat gtcgcccgca
    12901 taaacaaagg cgatctgcgt ggccgatacc gccggctgcc gcatcatgcg cgcgtcaatg
    12961 tgcgccggag ctgacggctg ggcgaacgcg gccagttgga gcagcccaac actcgccagc
    13021 gcaacaaggc atgaaagcaa tccggtcatg gctctatcct gcattatttc cagcacggag
    13081 tccatcccgc gacgcggccg gatccggagg cgagttcggt tctgctttat cgccttgccc
    13141 gctggttttc ctgagcctat acgcaattcg ccatccgcta ccctcaagtg gagagaaggc
    13201 cattctcgat cactcgcagc acctcgacga ctccgcatcg tgaatgctat cgaattgcgc
    13261 caggcgctga acactctttt atgattctcc acgcgatttt ctgctcaaaa gccacctgcc
    13321 tcgtcatact cgtccctcgt ttttcttacc cagtcgtacc agggacgaac cttggacgca
    13381 ggagagatgg ctgccatgga gtggatgcag agtgaagcgg caatagtgct gaaaagttgg
    13441 agaaagagaa acttaaccag aaaactcctc ccttttaaag aaccgaggga cgtttgcgcc
    13501 gtgatcggca ggtcttattt tctagacgat ttaggacttt atggttacag taccgcgtcc
    13561 ttgatcagcc aaacatccgg agaaccttat ggagaaattc gaaaaaattg ttgtgctgga
    13621 caatgaagtt caagccgagt tggtgggcag cgtgttggaa gaacgagaca ttccgcacct
    13681 gctgcgcagc taccatgatt ccgcttacga cgggatattt caaacgcagg caggttgggg
    13741 acatatcgaa gcgcccgcct ccttccggga taaaatcctg gctgtggtgg aggatatcaa
    13801 acgccaggca tcctcggatg cgaagccggc gtaagacgga tagccgttcc cactgtcacg
    13861 ggtatggatt acggcgttgc gcagggggaa gttctggacg cgctccgcgc cgaccgagtg
    13921 gccaatcttc atccttcccg ctccgaagga aacagccacc ggacagccca tacgggcacg
    13981 cggcctgtcg gcgtctccgt gttttcccgc aaaccgcaat ccttcccgtg gcgtgcgatg
    14041 cgaaagaagc aggctaaagc cccttagaaa cctgaagatt atacgtgacg caacccgggc
    14101 cagccgctat tttctccccg ttccttgttg ataaactgaa tctgaaatgc ccatgcaaac
    14161 gcgatttgat ctgacacaag ccgacattcc caaggcctgg tataatctgc tggccgactt
    14221 tccggaaccc ctgccgccgc cgttgcaccc cggcacccga caaccgattc cgcccgaggc
    14281 gctggaagcc atcttccccc ggaacctcat ccagcaggag atgagtcccg agcgttggct
    14341 ggaagtgccc gaacccgtgc gcgacattta tgcgctgtgg cgtcctacgc ccttgctgcg
    14401 cgccgtgcgc ttcgagaagg cactccaaac cccggcgcat atttattaca aatacgaggg
    14461 cgccagcccc gccggcagcc ataaggtcaa cacttcagtc gcccaggctt acttcaataa
    14521 agccgccggc actaaacgcc tggccaccga aaccggcgct ggccaatggg gcgcggcact
    14581 gtccatggcc tgcaagctgt tcgacctgga gtgcaatgtc tacatggtca aggtcagcta
    14641 ccagcacaaa ccctatcgcc ggatgctgat gcacacctgg ggcgcaacgg ttcatcccag
    14701 cccgagcgat caaaccgagt ttggccgcaa gctgctggcg gaagatccgc attgtccagg
    14761 cagcctgggc atcgccatca gcgaggccat cgaggacacc atcaaacatc cgcacaccaa
    14821 gtattccttg ggcagcgtgc tgaaccatgt ttgccttcac cagtccgtca ttggccagga
    14881 agccattaaa caaatggaac tggccggcga agagccggac gccatctttg gctgctgcgg
    14941 cggcggcagc aactttgccg ggctggcgtt tcccttcatc ggccggaaaa tcacccagaa
    15001 aaagaactac cgcatcgtcg gggtggagcc ctccgcctgc ccgacgctca ccaaaggcga
    15061 gttgcgctac gactttggtg acaccgccga aacgacgccc ctgctaatga tgtacacgct
    15121 gggccataaa ttcatgccgc ccgcaataca tgctggtgga ttgcgctacc acggcatggc
    15181 gccgatgatc agtcatgccg tcaaactggg tctgatggaa gccgaggctt atcaccagac
    15241 taaagtgttc gagatgggaa cgctcttcgc gcagaccgaa ggcctggtgc ccgcgccgga
    15301 atcggcccac gccattgcgg cggtggcgga agaagccatt aaggcccgca aggccgggcg
    15361 caagcgcgtc atcctgttta atctatccgg ccacgggctg ctcgatctaa gcgcctacga
    15421 aacctaccaa agcggcaagc tgcaggatgt ttaaccacgc aacctaaaac caaaacacag
    15481 tcaaccaaac cattacccga agtatgagaa cacaactgac caaatcactc ggcagcctgt
    15541 tgattgcagg gctgctcacg ctcccggtat tcgtccaagc tcaacccacg gctcattacg
    15601 cgccgggact ggaaggcatc aaagcggcca cgctgccccc gcccggcctt tggctgcgcg
    15661 actataactg gttttattat gccgaccagg tgaacaaccg ccatggacgt aagattagtg
    15721 cggcggatgc ggaggcattc gtttatgtca acgcgccgcg attgctgtgg attaccgagg
    15781 ggcagccgct gggcggctac ttgggcttgg atgcactgct gccgttgaaa tacacgtgga
    15841 tcaaggatgt ggatcaaacc tttggcatcg gagatttctt ctttgagggc acctggtcgt
    15901 ggcatttgca gcagtgggat tttgccctgg ccgcaggcat ttgggcgccg acgggtgatt
    15961 tctcgaagtc ggacccgacc tgggcaggca gtggctactg gggggaaatg ctcaccgccg
    16021 gcgcgacgtg gtatccggac caggagaagc gctgggcatt ttcggtgctc aaccgctacg
    16081 agttcaacca aaagcagcgg aaaacccata tcactcccgg ccaggcctac acggttgaag
    16141 gcggcttgag ctacggcctc tcgaagactg tggatgtcgg cgccgttggc tactatcagc
    16201 agcaggtcac gtccgatacc cacggttcgg caaaagatcg cgaccgcgtg gcgggcatcg
    16261 gaccggaaat caacgccttc ttccccgaga tcacgctggg catctctttg cgctacgttt
    16321 atgagttcat ggccgagagc cgcctgcaag ggcatacttt tgccctgact ctgaccaagc
    16381 aactgtaaag cccgcgcgta aaaaagcgcc cttttcgatc acccgccggc ctgcgtggca
    16441 cgggttggcg gagccggggt cgctcgtcgg cccgcgcgtc ggctgaacgc tgtcggggca
    16501 gccaaattcc gtcggctgga cacagaaaga cgccttatga cagaccaaca atggaacgag
    16561 ctgctggcgg ctattgcggg caagacccag gccaagcctc ccgtagggtt cattattgat
    16621 tcgccgtggc tgcctaactg gtatggggtg gacatccagg actatctcac cagcgacgcc
    16681 ctgtggttcg acgccaacct aaaggccatc gaaaccttcc cggaggttat tttcctgccc
    16741 gggttctggt cggagttcgg catgtgcacc gaaccttcag cttttggtgc caagtgtatc
    16801 tttccccgga atgagttccc attcgcggat aaaatcatta gcgacgtcgc gcaaataggc
    16861 gacctgtccg agccgaaccc cgccaccgac ggactgctgc cctttatgct taaccgtctg
    16921 aaaggcgcgc gcgctcgcct cgaggatatc ggccacaaaa ttcgcttctc agtttcgcgt
    16981 ggcccgctca acatagccac cttcctgatg ggcaccacgg agttcctgat ggcgcttaaa
    17041 accgacccgg gcccggcgca taaactgctg cgcacgatca cgcagttcct caagaaatgg
    17101 cacgcgctcc aacgcgcgac tttcccctcc attgacggca tcttcctgct ggatgacatc
    17161 gtcggtttta ttggcgagga tgacttcaag gagttcgccc gcccctgttt cgcggagttg
    17221 tatgctggcg acgaagcggt caagttcttc cacaacgacg ccgcctgcgc caagtccatc
    17281 gcctactatg ccgacctggg catcaacctc tacaacccag gcatcttcaa cacgctcgcc
    17341 gagttgcggc agttgtccgg ccatcgcctc acgatcctgg gcaatatccc cccgcgcgat
    17401 gtgctggccc agggaactcc tgccgaagtg caggccgccg tacagcggct gcgggcagag
    17461 acccccgaca tgtcccggct catcctctct tgcgctggcg gaatgccgcc aggcgcaagc
    17521 acagagaacc tcaaggcgtt tattgccgcc gcccgcggtt aagccgccga cttcggcaag
    17581 gcggcgcgaa cggccgcgag ttgtccggcc gagccgagca attggttgcg gctggcgata
    17641 aacttgctgt attcttccat gaagaccttg cccggcgggc ggaaatcaaa ctccgccggc
    17701 ttgtcgcgga agaactcgcg atgcacgcgg gtccagacca ggcggccgtc ggtatcaatg
    17761 ttgatcttgg caaccccgag ccgggccgcc gggaggtatt cgttggggtc cacgcccatg
    17821 gaaccggcaa tctggccgcc ggcggcattg atacgatcca cctcagtctt gggcacgctg
    17881 gaactgccgt gcatcaccag agggaagccg ggcaaccgat ccttgatctt ctgcagcacg
    17941 tcaaagtgaa gggattgctt gcccttgaac ttgaaggcgc cgtggctagt gccgatggcg
    18001 caggccagcg aatcgcagcc ggtttgctgg acaaagcgct cggcctcggc gggatccgtc
    18061 agacaggcgt tgccttcctc aaccttgatg tcctcctcga caccccccag catacccagt
    18121 tcagcctcga cactaatgcc cttggcatgg gcgcgatcca ctacgcgctt ggtaatggca
    18181 acattctgct cgaacggctc gtgcgaggcg tcaatcatca cggagctgta aaagcccgag
    18241 tcaatgcagt cgtagcaggt ctcctcatcg ccgtggtcca ggtgcacggc aaagatggct
    18301 tccggaaaga tttcgtcggc ggcgcgaata atggcctcca gcatgcgctt gtcagtgtac
    18361 ttccgggcgc ctttggaaat ctggatgata aacggggcct ggctggcaag gcagccccgg
    18421 aacaggccca tcgcctgctc ggcgttgttg atgttgtagg cgccgatcgc gtatttgcca
    18481 taggcgacct gaaacaattg cttggtcgtg acgatcatat ggtatctctt tttttgaggg
    18541 gcaaggatgc cgggttccgc gggcaaatgc aatcacgccg tgcgaaaatc tgcggaggcg
    18601 ccctggtcga gtgccgaggc ttcccaggtg tcggcccagc ggaccggagc gcccgcgctc
    18661 gttcacgccg gctgaggagc gtcgttcacg cgcaggaatt cgatgcgata gttggcctcc
    18721 ggatgacgcc gggagggccc ggccacgagc atggcgaccg cgcccggcag gcgatgctcg
    18781 cggagccact gtcgggcaaa cgcacgccaa tcctccggat cctcggccag ttggaccgcc
    18841 tgcactccgt aggaaaaggc caatccctgg caaacggcgg cgctttggct tatggccacc
    18901 acccacaccg ccgggttgaa ccgcgaaatc atgcgcgccg tggtgccggt ccaggtaggc
    18961 acgaacaccg ccgcgcaggg cacgctcccc agcgcgtttt ccaccaccga aacaatcgcc
    19021 tccgccgtgg tggccggctg gcgctggctc gataacgccc acaggtcgtc gaggcgggta
    19081 ggtggccggt gcgcttccgt gaacgccgca atcttcgcca gcattgccac cgcttcctcc
    19141 gggtagtgac ccatcgcgga ttcgcccgac agcatgatgc aatcggtgcc gtccaggatg
    19201 gcgttggcca catcagtgga ctcggcgcga gtgggcaggc ggttcaccac catggactcg
    19261 agcatttggg tggcggtgat gaccggtttg cccaccaggc ttgcccgggc gatgagttgc
    19321 ttttgcaaaa ccgccatttc ctggatcggc acttccaccc ccaggtcacc gcgcgccacc
    19381 atgatgccgt ccgtcgcctt cagaatatca tccagatgcc ggagcgcgtc cgcgcgctcg
    19441 atttttgcga tgaggaacgg atgcttgcca agcgcgctgg ctgccgcccg caccgcctcc
    19501 atgtccgcgg cggtttcgac aaacgactgg ctgatcgcat ccacgcccgc ccggagcgca
    19561 aactccaggc aatcccggtc gtgcgcggtg aacgcgctga ttcccaggtc aatgcccggc
    19621 agattcaggc ccttgcggga ccgcagttcg ccaccgacgg caacccggca atgcacctcg
    19681 cttccctgca cctgctccac caccagttgc accaacccgt cgttgaggaa cagccggttc
    19741 cccggcttca ccacccgggg caaccgctcg aagtttgtcg cgacccgctg ccggttgccg
    19801 acgatatcct cgttggtcag tgtaaagctt tcgcccgcgc gcagttcaat aggcccgggt
    19861 tcgatccgac cgaggcgcat cttggggccc ggcaggtcgg ccatgatggc gacgcgccgg
    19921 cccgtgacct tttccgcggc gcggatgcgc gcaatccgct cgccatgact cgcgaggtcc
    19981 ccatgtgaaa agttcaggcg cgccacattc attccggcgc gcaggaggcg ctccagcatc
    20041 gcgggcgaat ccgaggcggg gccgatggtg gccacgatct tggtcttatg gtcgggcaat
    20101 agcataggcg cagcgaatga aatcacgtcc accccgagcc ttcaacgaca gacttgccgg
    20161 cgagccgcgc tccgggaggc aaccacgccc gcactcccgc gggccggggt gccagccccg
    20221 ggtcagttgc cccggcgcaa tccggcgaaa cgaacggcgg cgtcctccca ggcggcggcg
    20281 gggccgggct taaaaacagc caccttgctg gacttgcgga taacctcgcg catctcggcg
    20341 agcgttgcca gttcaccgct ggcgcgcacc tggaccagca ggttgcccat cgcggtcgcc
    20401 tcgacagggc cggccaccac cgggcgttgg caggcgtcgg cggtgaattg gttgaggatg
    20461 gcgctctggg aaccgccgcc gacaatatga atgacctcga tacgattgcc ggtaagttct
    20521 tcgagccagc ccagcacctc gcggtacttg agcgcgaggc tctcgtaggc gcaccggacc
    20581 aactcgcctt cggtccgggg cgcgggctgc cttgtttccc ggcaaaaggc ctggatggct
    20641 ttcggcatgt cgggcggatt gaggaaccgc gcgtcgtccg ggttgacgat ggaacggagc
    20701 gccggggccc ggtcagccat acgcgcgagc tggccatagt cgtatttccg gccgcgggca
    20761 tcaaaggcgc gtttgcactg ctgcacgagc cacaggccca tgatgttttt gagcagccga
    20821 taggtgccgt ccaggccgcc ttcgttggtc agattcaacg cctgcgtgcg cgcgctcaag
    20881 gaggccttct gaatctcgac ccccatgagc gaccaggtgc cggagctcag gtatgcccag
    20941 ttggctttgc ccgtgtgggc cgtgggcacc ccggcgaccg cagcggcggt atcatgcgta
    21001 ggcggcgcca ccacgttgat tttgcgcagg ccggtgcgct ccgcgacgcc gggccgcaac
    21061 acgcccaacc gggtgccggg cggcacgact ttcggaaaaa tgcgcgtggg caggccaaac
    21121 tgtttcagca ggccggtggc ccagttgcgg gtcagcgggt gcaggcactg ggtggtggtg
    21181 ccgttggtga attcgaccac acgggagcca cacaacgccc aatggatgaa gtccggcatc
    21241 aacagcagcg cgtccgccct ttcgagcatc ttcggagcgc cctgtttatg ggccagcagt
    21301 tggaacaagg tgttgaactg catgaactgc aggccggtct gggcaaagat tttcgcccga
    21361 ggcacttttc ggaacgcctt ttccatcatg ccgttggtgc gggcgtcgcg atacgcgaac
    21421 ggctgtccca gcatttcgtc gcgctcatcg agcagcacat agtccacccc ccaggtgtcg
    21481 gcaccgaccg atacaatccc ggggccatac ttccgggcgg ccaggctcag gccgttctgg
    21541 atttccgccc acagccgcac gacgtcccag cggagcgagt cgcccagata gacggggcca
    21601 ttggggaacc gatgaatttc ttcgagacgg atggtcttgc cattccaaag gccggccaca
    21661 aggcggccgc tttccgcgcc aaggtcaatc gccaggtagg tgttaggcat aaagcgtcag
    21721 gggactgggg ttgcgggatt cgtagtgcgt tacgcgccgg gcatgggggg cgggccgtca
    21781 ggaggtggaa tcggcgggcg gcggcacgct ggcggacttc tcctgactca gcttgccact
    21841 gtaagccgcg attaccgtcg aagacaggag cagcaccagg cccagggcca gcaggccgcg
    21901 cgtgcggctg ctggtgcctt tccattcccc gaggaagatg cccagcagcg tgctgaagag
    21961 gatcgcgctg gccatcagga ccgcccagcc gatgtaggcc atcttgccca tcgccggttc
    22021 gccggttttg aagcagatga actgcgaaca ccagatggcg cccgccaggc cggcaaagag
    22081 gaagttgccc agcagcggcg catccttccg cgtgtagtcg ttagtggtct tattccgggc
    22141 attcaaataa aggcaccaga cgccgttaac caggaacccg ccgaacagaa cgacaaccag
    22201 aaccggcatc ccgcgccagg tggtcgaagt ggcgggcatg aggccctcag ccaactcttc
    22261 cagccgggcc ccgccctgca agccaaagct catcgccgaa ctcatcaggc cggagaaaat
    22321 ggccaccgtg atgcccttct tgaagttgta ctccgccacc gccttcttct tttccgcctc
    22381 cggcagttct ttttccttgg acatgccggc catgccgacc aacacaatgc ccaccagcga
    22441 aacgagcaca ccaaccatgg aagcgttggc cgaaggcgtg cccagcaggc tcccaacctt
    22501 gcccgcgagg atgggcggga ccagggtgcc ggcggcggaa cacagcccgc agccaatcgc
    22561 caaaccgagg cccacgccga gataccggat catcagaccc caggtcagtc cgcccacgcc
    22621 ccacgccgcg ccgcacagac agcagtaggc cagggtgcgg gccggcgctt cctggagcac
    22681 cgacagcacg ttgggcgagg taatcatcgc cagcgcccac ggcaccacca gcaggccgaa
    22741 caccgcgtag atgaaccaat agctttccca cgcccagttc cggaccttct tgaacggcag
    22801 ataaaacacc gccccggcca gcccgcccag cgtgaagatc attaccccca gcgccggatt
    22861 cgttgtcgct tgcgtcgcgt ccatcataaa gcccccgatt cttactggcg cttggacagc
    22921 acgtcccgct cgtagcgttt cacttcggcc agccaggcgt tgccgacggg cacgttcatt
    22981 tgctggcagt agtaatccca taccgcgccg gcgggcagga gcttggcgtc ctccatcagg
    23041 gccaggcgcg aggtgtagtc gccctcggcc tcgaactgct tgagcttgtt aatcggcgtc
    23101 accagggcaa gcaggagcgc cttgaccata ttgcgcgtgc caatcaccca cgccgcgacg
    23161 cgattgatgc tggcgtcaaa gtagtccagc ccaatatgca cgcggcgggt gtagtcgccc
    23221 caaacgatct cctgggcgat tgcctgcagc tcgtcggtga gggtgaccac gtggtcgctg
    23281 tcccagcgca ccccgcggct gacatggagg gcgatctccg gcacgtaatt gagcaccgcg
    23341 gaaatcttgt cggcaatgcc ttccgtcgga tgataatggc cggcatccag ggtcagcagg
    23401 atttgccggc tggccgcgta gcccaggtaa aactcggacg aaccgggcgt gtagctttcg
    23461 gcgccaatgc caaagagctt ggactcgacc gaatccaggt tgagcttccg gtcaatcttc
    23521 ttcttgaaga ccgcgtccag ggattggatc aggcgctcac gcgggccgcg gcggtcggcg
    23581 ggcgtgtcct tcatcccatc gggaatccac aggttggtga cgcaggtttt gcccagggcc
    23641 ttgccgatcg cggcaccgat ttcacggcag cggatgccat gttcgatcca gaacttgcgg
    23701 atgcccgcgt cggcgctggt aagcgtgaat ccacccacca ccttcgggtg agcgaaatag
    23761 gtctgattga agtccatgcc aatcttgagg gccttggccc aggcaatcca gttcttgaac
    23821 tggtcagcgc tcaacgcatc gcgatccacc ttccggccgc tcatctcggc gtagcaggcg
    23881 tggaggttga accggtggcg gcccgggatg acagaaagcg ctttctcgaa gtcgccccgc
    23941 agttcattgg cggtgcgcgc cttgccgggg tagttgccgg tgacggccag cccgccgccc
    24001 agttcctggc cggcgttctc gaagccgccg acatcatccc cctgccagca gtggatcgaa
    24061 atggggatct gcgccagttg tttcagggct ttgtccgtat ccacacccac ctgggcatac
    24121 cgttctttgg cgagctggta ggattgttcg atgttcttcg tattgctcat aaataatgct
    24181 tccgcagttg acaatgttgc gcggatatta tgcggcttgg cgggcgcgtc aacaaccttt
    24241 tccagcccgg tgcggccatc gtgattcggc tcgcgaataa gctaactttg aacttgcgcc
    24301 acaacgacca accccctaaa ttgccgcaag aacttacgaa tatattatgt ctgcactgct
    24361 tagagccaca tggcgagtcc gccaaaaaat tagagcgttt actttgattg aactgttggt
    24421 ggtgattgcg atcatcgcca tcctggcggc gctgctgttg cccgccctgg ccaaagccca
    24481 gcaaaaagca ctacaggcca actgcaccag caatctcaag caggttggac tggcttgttc
    24541 cttatacacg caggactaca acgattacct gcccggcccc tgctggtccg gcatgttttg
    24601 catctacaag gataacgcgc ccaaccagac aatcgccgag aatcccaaca agtatttcgg
    24661 cgctttggcg gcttacctca ccacttatct cgccagcccg ccgccatcgg gcaccatcgc
    24721 ctatacttcg aaagtaatgg tatgccccgc cggctggaaa aaaataccgg gcaaacaaag
    24781 ccttaacatc ccatcgagcg tcccggtgct gtacttctcc ccggatacca tctacacaga
    24841 tccttacaac actaaccaag tggcattcct gtttcccttt ggccgcccca acacccctta
    24901 cgccgcgccc cggaaggtca ccaacattcc gaatccggcg ggccagtggg ctattacgga
    24961 tgccgacaag tacaacgttc caccaggggc aacctacttc aactggctgc ctaatgagcc
    25021 tgtgcatggc actctccggc cggctatgcg ccagaccctt ttcttcgact ggcacgttca
    25081 atcgcggaag accattccct gatcatcccg gggcggccag ggcggcggaa gtggattggc
    25141 tggcgatgaa cgcgcgcgag cagcttcgcg cctttccgaa taacaggaca tcagggcaac
    25201 cctcgaatcg ggcttgccgg agccttatta ccgctttccc aatgaaaccg gccggactgc
    25261 gcccgggata aagccgggcg ctgtctcgat tcgggtaaaa gctgcgtggc cccagtagaa
    25321 gccaaggcag ccaggcatcg gggtcaaatc agcgggtagg gataaatgtc cgccggatcg
    25381 aagcggcctg gggcaaactc gagggcctgg ccggcttcgg ccgtttgcaa tgcgtgcgcg
    25441 ataccaggct cgataaacag caggtcgccg gttgccagag gcaccgtggc gcgctcccgg
    25501 gtctgaatat cttccaccac caccgtcagc cggccttgca gcacataaac ccgctcccgt
    25561 ttgattcgat gataatgatt cccccgcacg ccgccctcgc ggagttcaag agtcgccagg
    25621 tagtggatcc cttcgtcaga atcatgcact tgcgccagtt ccccttgcgc caaccgcagg
    25681 cgcttgagcg tgggcgcatc gggaccggga cgcccggtga gaatcggcag cggccaccgt
    25741 acgactttgc ccgccaggta tttaacttcg gccataagga tttccaggtt cgcccagcca
    25801 taccacattc cgcggggccg ccgcaaccgt ggcgttttcc ggcggcggcg caattttcgc
    25861 attattgact tgcagcgcgc ttcagctcac gctgccgcca tgaatggcga tcaacgtgcc
    25921 tccgcagttg cctctgaaat gccgtcccgc tgccgccggc gccggttgcg gctctgcctg
    25981 gcgctggggt tcctggcgtg gggcggagcg gcggcacggg ccgattggcc ggagtttcgc
    26041 gggccgcggg gcgacggcca tgccgtgacc ccgggcggcg ccccggttgc cggcctgccg
    26101 ctgcattgga acgagaccaa caacgtcaaa tggaaaaccc ccatcccgca tcgcggctgg
    26161 gcgaccccgg tcgtaatggg cggccaggtc tggctgacca cggcgaccct cgaggggcat
    26221 gactttttcg ccctcgggct ggacgccggg acgggccgga tacgcttcca cgagaagctg
    26281 tttcatagcg ataatcccga gccgctgggc aacggggctt cgatgaattg ctacgcgacg
    26341 ccctcgccgg tgatcgaacc gggccgggtc tatgtccatt tcggcagctt cggcacggct
    26401 tgcctggaca cgcgctccgg gcagacgctg tggaagcgcg acgacctgcc ctgccggcat
    26461 tatcggggcg cgtcttcgtc gccggttctc ttcgaaaacc tgctcatcct cacgttcgac
    26521 ggcgcgaacc tgcaatatct cgccgcgctg gacaagcaga ccggccgcac ggtgtggaag
    26581 acggaccgct cggttgcctg gaacgacgaa cacgaaccgg gccagatggc gcgcgagggg
    26641 gaccaccgca aggcgcacgg cacgccgctg atcgtcaccg tggcgggcaa accactcctg
    26701 ctcagcgccg gcgccaaggc cgcctatggc tacgacccgc gcaccgggcg cgaactgtgg
    26761 aaagtccgct acaacgacta ttccaccgcg ccccgcccgg tctttgaccg cggcctggcg
    26821 ttctttgtca ccgggctggc caccaaagag ctttgggccg taaagacgga tggccgcggc
    26881 gacatcaccg ataccggggt ggcctggaag ttcaatcggc gcgtaggcat ctacgcctcg
    26941 ccgttgctgg tggacggctt gctctacacc gccgccgcgg agaattacct gacctgcgtg
    27001 aacgccgcga ccggcgagtc cgtctggagc gagcgcatcc ccggcaagta tgccgcctcg
    27061 cccatctacg cggacggccg catctacctc ttcaaccagt ccggcgtcac caccgtggtc
    27121 aagcccgggc gcgccttcga agtcctggcc accaacaacc tggcggacgg gttcatggcg
    27181 tcgccggccg tgtccggcaa ggcgttctac cttcgcacca aaacgcacct ctaccggatc
    27241 gaacagtgaa ccgccaccgc ccggcgggcc gggcgggcgg ggacgccgga tgcgctaacg
    27301 cgccgggcgg cgcatctgtt cgagaagatt gcgggtgatt tgccgcaccc gggaatcggg
    27361 cccggcaggc tgggtataga cagacggacg gacgtagccg ggcggctcgc tcaggccgtc
    27421 cccccaccag caagtggaag cgttgaacac gaaattgccg cgcgggccgg gatagatggt
    27481 cgcggtgtaa tggccgtggt ttaatacgcc cggcttccat tgggtcggcc cgccggcgac
    27541 gatctccagc cccgggatcg cggcggggtc gccatgccat tcccagccca ccaggcccgg
    27601 gatgccctcg ccgcgtttca tgcccgtgcc cgcaaacaac caatgctcgg gcttgaggca
    27661 aatccagtcc gcgccgccgg agaacgggcc caggttgcgg gcgcccacca gttccgccga
    27721 actggggctg gtgtgcggca gcttttccat gccgcggatg ggcttcgggt cgcgcgggcc
    27781 gtaaaagtcc acccgctcat acgtgcgcag gcggccgcgg aagtcgggct ggaattcgat
    27841 gcgcccgcag caggtattgc cggagagaaa cgccacgttg agtccctggc ggatggcatg
    27901 ctgcaagtgc cggaacattt ccagggagta atactcatca tgccccaccg aaaggaagcc
    27961 gcgcgcgcgc agcaatccgg ccgggtcggc gtgggtgtcc aggttcgaaa tatagctcac
    28021 gtcgtagccc tgctcttcca gccagtacgc caggggaaat tcccacagga agaactcgcc
    28081 cgatccggtc gaaagcggcg cgtccaatat ctggcaatac ttgccatagg gccggttgaa
    28141 gctcacggcc acgtcattgc cccaatacca ccgcgccagg ccgtcgtcat acaacgaaaa
    28201 ctggttgggc cagcggttat aggcctgcca ggtcgtgtcc gaacactgaa aaagaaaatc
    28261 cgcgcggcga tcgtcgcgca caatgaagat gacgtagctc tgccagccgg cgggctgcgc
    28321 ggtcagcttg ccgacataga cgccgctgag ccaatcggcg gggattttga gcgcggcgca
    28381 cgcctcccac tggcaatccc gcagccgccg ggggccgatg ggcgggtcag gctgctgcgc
    28441 gccctggaac gggcccagcc gcttcatcaa ccggccgccc gcgccgccat aatacccgag
    28501 ccgataaaga tccagcgtga aggttgcggg cgggttggtg ctgacgtgga aggtcagcgt
    28561 ctggcccgcg cgaatactgg tatgcgagca atagccctcg atccaggggc agcggtattg
    28621 ggtcgcggga tcaataccgg ttttggtgag cagccaatcg cgcgttcccg ggcgctggtt
    28681 ctctctccga atgagcccgg cggacgagcc cggagaagac gaacgcggga gcgcgcagcc
    28741 ccccagcgcc aggacactgc cggcgctcag catcccctgg agccattccc ggcgggacac
    28801 ggcggtggat tgagttcggc cgggacgcgg ggcgtcccct cgggaaagag gatgcttgtt
    28861 catgctcata tttcgcaggt tatggccagc agcatggagc cggcccgccc aaagtcaagc
    28921 cccgcctccc ggcccgccgc ggccgccttc gggccggcgg ctcaggccag ccagggctcc
    28981 cgccgcgcgc gcgccagcag gcgatttgcc tccgcgtcgt ccgggaagac ttcgcgcgcc
    29041 ggatcccacc gcaggctgcg gccgagctgg tatgccaggg ccaccaaatg ccccagcgac
    29101 gccgagcgat ggccgatttc ctcatgggcg ctcggccgcc cggccgagcg caggcaatca
    29161 aaccagttcg catgatgatt attcgcgcca atattcacct tgcgggtcgt caggcccgcc
    29221 tcgcgcagga ttccctccgg gccgccctcg atcggcccgg cgccggacat ggaggtgatc
    29281 cagccgcgct cgccgacaaa caaaccgccg aacaacccct ccagccgcgc ctcggcgggc
    29341 acggcccggt agagttcctt cacctgcccc caatggtcca catgatgcag cagcacgccg
    29401 ttggcgtagc ggcaggtgag cgtaggaaac gccccgctgg ccggatgaag gatttccacc
    29461 gggccgccgg tctccacgcc cagggcgtat tgcatcacat ccgccgcgtg cgaatggtaa
    29521 ttcgtgatgg gccccgcgcc gaaagcgcgg cagaacgtcc acggcaccac gccgggaacc
    29581 gggttgcggt ggtaggcggc gttgtagtcg tgccaggccg ccggcccgac ccacaaatcc
    29641 caatccagcc ccgcgggcac aggctcggcc ggcagcgccg ggtcgagcgg aatataggat
    29701 ccgcccaggg tgggaatctc gattttcatc cacagggtga aggccgcctt cactttgccc
    29761 agcccgcccc cacgtacgaa ctggcacacc tggcggatgc tgggaatcga acggtattgc
    29821 gtgccggtct ggaaaacgcg cgcgtagcgg cggaccgtct cgaccatttc gcgcccctcg
    29881 cgaatcgtga gcgaaaccgg cttttcgcaa tacacatgct tgcccgcctg cgcggcgtga
    29941 atggcctgca aggcgtgcca gtgatccggc gtcgcgatga ccaccccatc aatatccgac
    30001 cgcgccagca attcccggta atcgtggatg gccgcgcagc cgtgaaacga gcccgcctcg
    30061 cgccgcgccg cgctgatttc gtccacccgc tgcgccccct cctcccgccg gacgcggtcc
    30121 acgtcgcaca cggccaccag ttgcgtctgc gaatgcccgg ccaggatgcg cagatgcccg
    30181 cggcccatcg cgcccaggcc gatcagcccg acctggatgc gctcgctggg cgccaccgcg
    30241 ccgttccggc cgcggacgga cgccgggata atcacgggcg ccgccagcag ggaagcggcg
    30301 ccggcgcgca gcagaaaggc gcggcgcgtc aaacccggtg agttggtttt tggcataagg
    30361 cggctacttt ctccgggcga ggtcaaggct gacccggttg aaaaactcga tgcaccgggc
    30421 gatgtccggc atggaagcga gccagttgtg ggaatactcc agcccgaaca tcgtcggctg
    30481 gaggcccagc cggtgaattt cctcgagcag ggccgccgta tttcccacgc cggcgcccca
    30541 cggcacgtcg tggccatccg cgccgcgggc gtggagatcg tgcatctgga gcgtgagcag
    30601 gcgatccttc agttggcgca cgcccgcgag ggggtcaatg ccggcccgca tccagtagcc
    30661 caggtcggcg caggcgccga ggcgctggct ccggcccgcg cacgctttca ggatgcccgc
    30721 cggattccaa taagcgggag aagcctgggg atcatgatta tgcaaggcca ccttgagatc
    30781 ataggcgtcg cagaacttct caatcatatc gagcgactcg ggtttgggct ccgacatgaa
    30841 ggtctcgatg cctatctggc gtccgaactc gaacaccttc cggcagccgg cttcatcgcc
    30901 gggaatctcc tgcaggtagt aggtgagcag ccggacgccc gcggcgtcca gtttgaaccg
    30961 gatctgccgc aattcctcat cggaaagccc cggctcgaag ttccgggcaa tgccgccgcc
    31021 caccttctga aaactcagcc cgcccatgaa gggcagcccc agcgccgcgg ttttctcaat
    31081 ggcctcgaag agcgtgaact tatgaaacgt gtaggcctcg atcccgagcc gccagccgag
    31141 cttctcctgc gcgcggaggg ccggcgtcag ccatgcgctg ggaacggtcg gcgcgggaag
    31201 atcgcccaga ataaactgcg ccgcggccag gtagaacgac agcagcttcg gatcgcggaa
    31261 cacggcggga tggtgcgcaa tggtgcagta aaacgtccgg ccgcggccat aatggcgcac
    31321 ccatgccagc gcgtaatcat ggtcggcgcg ctccggcggc cggccggcca ggtcggtctt
    31381 gtccgtgtca atgcgcagca ggacgcgcac gcgccggcgc gaatacgggt cctgcacccg
    31441 gaaaaactcg tcccggtagg cgaagtcccg gccgcccagc gcctggttaa gcgggtgctc
    31501 cggctcttcg agcttgatga aaacctgctc gtcactcgcc cggtgattcg ccccccgcgc
    31561 gccgatcatc aagccaaact ccggccaatc ctcgaccgcg cccggccact gggtaaaagc
    31621 gaccgtgctc ccatgcaccc ccatcaggcc cccgccgcca tagacaaact cgaccaggct
    31681 ttgccgcagc gcggcatccg tgaacaggtt gcccacgcag ttgttgaaaa agacgccgtc
    31741 aaactgccgc aggcgctcgg gccggaacac ttccggatcc cggctcacca ccgtctcgaa
    31801 ggccccggtc cggcgtccca tcaactcgaa cgccaggttc gccgcgggaa tggagccatg
    31861 cccgccgtag ttcacgttcc ggtcaaagat gagcagtttg cggggccggg cgggccgcgc
    31921 cggggcgcgc gcgggaaggg cggcttcgat caactcgcgc tccgcgccag cccccggccc
    31981 cgacgccgcc cgcagcacgg cggggccgca gccgagcgcg agggcctggg cggcggcccg
    32041 agccaggaaa gcgcggcgcg tcaacggctt cacccgcgcg tccgggagaa ccgccaggga
    32101 gcaatcgtca gggcacgtat gcggcatgcg aatcaggatt gtgcgcatga ccccgcccag
    32161 gtcaagcccg gggctggaaa ttattcgcca cgcccgcgcc ggacgccagg gcgcgcgccc
    32221 ggccggtccc cggaacggcg gcagccgaaa gttggccgtc cgccgggccc ctgtcccacc
    32281 cgcgggcacc gagtgtcccg gatcaggaca ggccaaaccg cgcgaggcag cccttttgcg
    32341 cgccactcac ccggccgccc tcctctcccg gcgattggct cgcaaactgc ggcgaccgaa
    32401 gcaaatcagc ctgtggtccg gcccgatgac cgggcgaaaa aggcgaaccg cgaagggaac
    32461 tctccggtct tacctggaac ggaggcccgc cctgcaacgg aagcccggac gacagattaa
    32521 ttgcgacaac tatggctcag gccaaacgga aacaggtcat ggacagccgg aaacggattc
    32581 tcctggtgga cgaccacgcc gtggtccggt tcggcatcgc gcagcttgtg aaccagcaaa
    32641 gcgacctggt ggtttgcggc gaagaggagg acgccgccaa cgcgctgcgc gccatcagcc
    32701 agctcaagcc cgacctggtg atcgcggaca tctccctcaa agacagcagc ggcctggagc
    32761 tgatgcgcaa tatcaaagcg caacacgccg ggctgccggt gctggtcgtg agcgcgcacg
    32821 atgagtccct ctacgcggag gtcgccttcc gcgcgggcgc gctcggctat ttgatgaaag
    32881 ccgacgccct ggaaaaagtc ctggtcgcca ttcgccgggt gctggacggc aatgtgtatg
    32941 tgagcgacgc gctggcggcc cggctgctgc agcaacaagt ccgcggcgcc cggtgcatcc
    33001 aggaatctcc cgtcaaaggc ctcagcgacc gggaactcga agtcttccaa ctcatcggcc
    33061 aatggaagaa gacccgggaa attgcccagg ccctgcacct gagcatcaaa accatcgagt
    33121 attaccggga gcaaattaaa caaaaactgg atctgaaagg caccgccgaa ctcacccaat
    33181 acgccaccgc ctgggtgcag cgcgaaaacc ctgcctgatc cacgcgccga aacgccgccc
    33241 ggtttgtcga acgctcccct ccgccccggg ccaactccag tgcccctcgc aaaaaggcgg
    33301 ctatacgttg gatttgggct cgtgggtgct cttgcatatg ggtggagcgc aggaagtggt
    33361 agccccggat tacacccagc cgtaacgatt tagttggtca attcaatcgc ccgcccctct
    33421 attatcggat tctgggcgga ctcgaccgcc caaaccgcag ccccgacgag ccccttgttt
    33481 agccaattca agggcatggc tgctgaatcc caagcctttc gccgataaaa tcaaaagttt
    33541 aggcataaag aggggcaaaa catgagcaaa acctgaatca tgccaacttc ggcgcacctt
    33601 ggacgcccct tggatttacc ctgccttcag gccgtgtaca aggggtgtac
//
LOCUS       DAOPQG010000030        33496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1721_length_33496_cov_15.582279, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000030 DAOPQG010000000
VERSION     DAOPQG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 33496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33496
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_33496_cov_15.582279"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..3451
                     /locus_tag="PKI07_05060"
     CDS             314..3451
                     /locus_tag="PKI07_05060"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOC50455.1"
                     /translation="MACNLVTAATFRQLQSRLLEDLRRAGAESGWSPKWVVVPSASLA
                     NHLRFELSRGASTTALANVRVVNVPQFAQRLALSLLRTATPPWGALLDLLLWGLVEDL
                     PPQSPLASLRTISGGPGLLRTAFVDLAEAGLGPGEIWKLEELAGQPDMPSRDRELLLL
                     YVRWVQLLQERQVEWAPLVLQRLPGRLEEAEDRELAAALGAEAGQSPTLYIYGFYDWI
                     DVHLEWLGALSRRVATTVYYPWMGAPEGAHPAFEFTGPVRQYLRGHFAFAGEDAVAEP
                     PEVLGQFFLETFPEGSIGPQPDGLSYQRAAGTRAEVISAAIRIRAWLDDKDNPLAPEE
                     ILVVAPQADAYADVMQEIFAAFAIPVRIADVVCGPTPESAILRMLARIWEEQAPAEWV
                     LGLLRACPDARVARGVNLDQFEGKVRELGIWGGAAWRRVLADDEFPARGEGSRRPPVQ
                     FNAAEKALIREIIEFVPAGNAEAVPRITARDARAMLDRLQKDWMSDPTPLEPLVRATE
                     ELARWRPSLAIERRQWTQLVAESDGVRRQRDPRSRAVLFLPMMRARGLTARGIVILGL
                     AAGQIPFRVPEDPLLSELASARLAEWANQIGHRLPPKARLTEELVLLFSLINTAGQRV
                     HWVVPETDAAGKAVAPTPWVQRYFNRWENQEPKVADPLSRRIARAPFEQAIYLKKLDP
                     KRGEFLPPALALFVNPKLAARGDPDAARSWLGESIARRGKKPEWSGSIQCQPDFRNGR
                     VNVTSLEALAQCPFRFYGEQVAGWKALEALSLSHELAALARGSLLHKLLEEAVRPHLR
                     TSRVAEIAAEMLAQECAKLHTLAQRLPEFSPATAAALALLPEVFQQAARRQMVRLAAA
                     YFEAALNHPAVPQDVEVAVAKEFPGIPGLMVAGKIDRVDHNEARVELLDYKSGKMPSD
                     YRKRVKLGWQIQAVLYPWLSGQNDACFRYLFLGRAAAEEGDAKGAPEAAGFLSELAPL
                     LQAGHFIPTSNQAMEELGLSKVSPCRYCALASACRRFEPGAAAKHARLFEKLAPARCA
                     SLRQAVNGKPPEKTD"
     gene            3451..6846
                     /locus_tag="PKI07_05065"
     CDS             3451..6846
                     /locus_tag="PKI07_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC50456.1"
                     /translation="MIPDARQRREAVLTSGHAFVWASAGTGKTHTLTLRALYLLLTAP
                     FDSRAQGTECRLLYGGASRAERLRAARAVLRRFVLTTFTRKAAAEMQTRLYGYLDALA
                     SAASLTALRERFPNDRQLREVVEATLGRLGGGEPFSRLQAGAEALGELATELRVCTLH
                     SFAASLLQRHSIAAGIPPLAQFAEEDDPTLMNVGSQVVERWWQKVLAEPELLQDLEAL
                     IPILPQEDMREWFEAIYQHRRIADEMDFPAPDNHAIHALLAASRQLAQELAGAPSRAT
                     RVIQAGRELQAILASISNGQAFAWRDFCGYLERQSEVLFSSKRSQAVQKAIAALGAGS
                     RFYEDDGLIYGLALQKCLAEEYPEDWARWRRLAGRFAKWAEDAAVRELGVVTFDDMVR
                     LAARLLRENDAVRREEREQLWAILVDEFQDTDPLQLDLLESLLRRQPGDPELMGFFVG
                     DLKQSIYRFRGADLPAIRGFVSRYSQVVQSTEVGEFQLTANFRSRPPITKFVNYFFER
                     CVPLPNYPNERLEPVRKGATPMPEWRRLALAQQRGAVGARLESAGRETVRLIAEHHDQ
                     SGQDDAAYRDIIVLVSTHDELDMLLPLLEKAGIPAVSGGARTFCQQPEVLDVLNLLIA
                     AQNPLDTLATGALLRSPLFGLSDVQIYGLLARLEPGEIFHGDAALPADLPAPARGRIE
                     QLRRLVRERPGTALAMWLSQLREFIPMGLYAQQNIEGRGVARIDQVLAAFRRAVELGS
                     LPPISWLLEQRDRARDRFDAKPGEDVSVTDESTAAVRVLTIHKAKGLQGKYVIVLGWQ
                     KALDKLKPREESSCHFQLTDADGHTVGGFKLPWGRLEVVSTDYRKAQKLNQQYEAEEA
                     QRLLYVAATRAEDRLVLLSPHCQAIPQGQLVEIPSPLQQDGGAGEACGGTLEVIVTLP
                     PEPIQRPSGSAVRLRSPDAYAQLWRARLERAAGEAEPLLHQPSRLEPVEAEDKADLPD
                     YVREKIDMASARAMELGTVVHRYLERHLQEPGYDAAKLEAVVSEINGGVSFPETIEKA
                     RVVLTDFYGGALHQRACRGRIEAREAPVFASWEGKAWSGVIDLVLREGDAVIGVDYKV
                     MKMPKQLPAEYEQQRRVYQEVLRRLFPGQPVSFEFWWLINSPR"
     gene            7192..7266
                     /locus_tag="PKI07_05070"
     tRNA            7192..7266
                     /locus_tag="PKI07_05070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7224..7226,aa:Thr,seq:tgt)
     gene            complement(7297..7782)
                     /locus_tag="PKI07_05075"
     CDS             complement(7297..7782)
                     /locus_tag="PKI07_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOC50457.1"
                     /translation="MSSSPLPASRFQRILFCTDFSESAAAAFPFALEAAARRPDATLY
                     LMHVIHEPDAQYWKTQLAEVENINAEAKKTIDAKIAADYLARVPPGMNLQTVFRIGPD
                     AATILEFARSERIDVIVLGRHGHGGMGKALFGSVAEKVVRKAECAVLVVPLAYAKAAP
                     A"
     gene            complement(7793..9094)
                     /locus_tag="PKI07_05080"
     CDS             complement(7793..9094)
                     /locus_tag="PKI07_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOC50458.1"
                     /translation="MSPNEGALFGFALLLLLLAGSMPVGYVMAAVGLVGFAVMVSPAA
                     AFSMATIDLYTTFSDYNLTTIPLFVLMGQIAFHTGISRGLFHAAYHWMGRLPGGLAMS
                     TIGACTAFGAICGSGPATSATIASVALPEMRRYRYDMQLATGCVAAGGGIGMLIPPSI
                     VFIVYAILTEQSIGKLFIAGVAPGLMIALLFWAVIYFECKRHPHLGPPGPVFSWRERF
                     ASLKGVWETLTLFVLVMGGMFAGFFTATEGAAVGAAGTLVIALLKRELTWRKFTQCLN
                     ESIRTACMVMVIVAGAIIFGKFLAVTQVPSTLAAWLSGLPLPGWGIIGLIMLFYLVGG
                     CFLDALALDILTIPIFYPLILALGYDIIWFGVMIVVVTMMGVITPPVGVCVYVVAGMS
                     RDVPIEKIFRGSMPFLGALVLAAILLILFPQIATFLPGLIK"
     gene            complement(9091..9618)
                     /locus_tag="PKI07_05085"
     CDS             complement(9091..9618)
                     /locus_tag="PKI07_05085"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOC50459.1"
                     /translation="MSSPPVPPSGPQSPWRLYDQALRALVMALAYVACASLMVMVLVT
                     SAEVVLRLFRLSLTGAYDIVKIAAAITIAAALPYTTAIKGHVAIEYFFHKLGRRGRIV
                     VDALMRLAGMALFSLLAWGCVDYGHSLRTRGEVSMTLQLPIFWVPYVLAVSCGLVVLI
                     KAYHLAHPGKPMIKP"
     gene            complement(9742..10767)
                     /locus_tag="PKI07_05090"
     CDS             complement(9742..10767)
                     /locus_tag="PKI07_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOC50460.1"
                     /translation="MKPWVLMAGAAGLAALCLTACKPAEPKETIKLSYSVFFPPTHVQ
                     CIAATNWAHEIQQRTGGRVQITVYPAGSLTKADQCYEGVIKGISDLGMSCFAYTRGRF
                     PLLEGLDLPLGYPDGATATRLANTIIQKYQPAELNDVKMLYVHAHGPGILASKKPVHS
                     LDELKGMKVRATGLSAKIVEALGATPVAMSQPETYEALAKGVVEATLCPIETLKGWKQ
                     GETIEYVVDATAIGYTTAMFVVMNKDKWAALPADVQQVFTEVSQEWIAKHGAAWDEAD
                     QAGREFVTDLKRQFIHLPEAEQQRWKAAVKPILDDFAKQAKEKNLPGEAMLADIQAGI
                     QASKTSQ"
     gene            complement(10910..11302)
                     /locus_tag="PKI07_05095"
     CDS             complement(10910..11302)
                     /locus_tag="PKI07_05095"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOC50461.1"
                     /translation="MRPPPHGPAPQTPRERVCAQWRGVDLAPLEQAQALRAQPVGTVL
                     TRVLATLRLDRRRSEAEIAKAWNDLLDPNLTAHAQPTGLNKGTLYVTVDSSVWLSEIV
                     RYHRKEILERLQHSFGRDLVRRISFQVG"
     gene            complement(11342..13417)
                     /locus_tag="PKI07_05100"
     CDS             complement(11342..13417)
                     /locus_tag="PKI07_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOC50462.1"
                     /translation="MYYGVSYHPEQWVYPYGGTPERPEAAWEQDIEWMQSAGINVVRM
                     GEFAWGLCEPEEGQFDFGWLGRVMDLLGKAGIKVVLATPTAAPPIWLTQKHPDILPVD
                     ERGLVKREGTRHAVCLASDKYWEYSRRIVEQMAKALGPHPQLIAWQIDNGVGGNFTEA
                     SFNEDARREWHLWLENKYETIERLNNLMGLRHWGQNVTSWKQVPMPMRAPTAHNPALV
                     LDWRRFCSDIIVGFVKMQADLLHQLTPQCPVTTNLRPLLHRFDHFDLAEVIDFVSIES
                     HAVLKPKPSGMACEIDMLRSLKKADIRTPDGDAGFWVMEQKAGSVNWQEVHALMRPGM
                     LRLFSYQLMARGAEAIVFFRWRQPRFGGEQFHGAVMPHRARATARVFKEVDQLGDEMK
                     LLSPTLKHSRVAAEVCLLYTHDNEWALQQATHRNQFFNLREHIQLFYNALHERNLAMD
                     FARPTEDLSRYKLVLAPSLHLLAGGEADRLKLYVQNGGTLVATFNTGLVDEYHQVPDT
                     GYPHDLTDLFGLEVVEFDHLPPGEENHLTFKGAFPASHMHPARLWCDLIEPGDCQVIA
                     SYAKDFYAGRPAMTLHPFGLGKAIYIGTQSHAHFYTDLVTWLRQMCNLHPPLRVPDNI
                     ELCQRQKDDANLYFLLNHQNSPVRVQFYKPMHDSLTGNAISGNYDLPPRGVLVLDEPL
                     EAKPAGETE"
     gene            complement(13557..14447)
                     /gene="metF"
                     /locus_tag="PKI07_05105"
     CDS             complement(13557..14447)
                     /gene="metF"
                     /locus_tag="PKI07_05105"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOC50463.1"
                     /translation="MQFIRDIYANGRTAGRPVFSFEFFPPKTDEGDRILFEKTLPALL
                     QARPDYCSVTYGAGGSTRDKTLRIVERIQRQHALTTVAHLTCVCATREQIGELLAQLR
                     ALGIRNLLALRGDPPGGGEFQPAPGGFEFARQLVQFIRAQGDFSIGVAGFPEGHTACR
                     AGRHADWRHLKEKIDAGADFVLTQLFFDNADFFCFRDHLTRELGVQVPIVPGVVSILS
                     GAQIQRFTAMCGASIPAPLAARLAELGGDDAAVAEFGIEYATRQCEGLLQADVPGIHF
                     YTLNKAAATVRILQNLGRAR"
     gene            complement(14611..14793)
                     /locus_tag="PKI07_05110"
     CDS             complement(14611..14793)
                     /locus_tag="PKI07_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50464.1"
                     /translation="MIRRLLGVDFAGLVPHHCPMPEQKNPGNPAEPRGVVPYNPGLYA
                     LDIPTANAGARTSCPL"
     gene            complement(15010..15924)
                     /locus_tag="PKI07_05115"
     CDS             complement(15010..15924)
                     /locus_tag="PKI07_05115"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOC50465.1"
                     /translation="MKPARLWQVSVRTTPEAEEAVAELLGSVLGPPASSYTDAETRET
                     VVSVYLEEKPAGWRGGVCPSALARGLRRIAASGLDLGPGRISLRRLRRADWAESWKRH
                     FPPLEIGSALLLKPGWSRRRPRKGQAVVVLDPGLSFGTGRHPTTAFCLRQLVARRRPG
                     EAQSCLDIGTGSGILAIAAARLGYAPVDAFDDDPQAVAVARANARRNRVAGRIGFARR
                     DLTRLPRRARRQYAIVCANLVAGLLREQRERLLARLRPDGVLVAAGILAAEYEQVRRA
                     FAAAGLRQIAARTQGEWRSGAFTRRPSS"
     gene            complement(16033..16668)
                     /locus_tag="PKI07_05120"
     CDS             complement(16033..16668)
                     /locus_tag="PKI07_05120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOC50466.1"
                     /translation="MKPAAAAALRLLLVRHAEVEADGQNIFGGRMDLALSPRGHEQAA
                     VLADYLRRQPLDAIYASPMKRVQQTLAPLLARGAPPPVILPDLREMDFGDWTGLSGEA
                     VQARFGVSAWSWLEQIENNRIARAEGFAALRERVAPCLRRIQDEQPGRQVAVFCHGGI
                     IRVVLALLLHWPLARLGAFEIAYASVTRVRLHPERVQLQLINYAPWREPGA"
     gene            complement(16688..17389)
                     /locus_tag="PKI07_05125"
     CDS             complement(16688..17389)
                     /locus_tag="PKI07_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC50467.1"
                     /translation="MTASADSVLVEVDGVEKVFRRGAEDIHVLQDLHLRVPAGEFLAL
                     MGPSGSGKSTLLNLIGGLDRPTRGTVRIAGERVDRLSDHRLAAWRARHIGFVFQLYNL
                     LPVLTAERNVELPLLLTHLTRAQRKRHVATALAIVGLAARARHYPRQLSGGEQQRVGI
                     ARAIVTDPTLLLCDEPTGDLDRKAGDEILDLLQGLNREHGKTIIMVTHDPHASARASR
                     TVHLDKGRICDQPVT"
     gene            complement(17393..18622)
                     /locus_tag="PKI07_05130"
     CDS             complement(17393..18622)
                     /locus_tag="PKI07_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC50468.1"
                     /translation="MSEPKPTLSDLRIERDAPPAASSWRWGAAGVLLALLLAGGVVFW
                     LQRPAARPVRTAVARAAADDGVERTVLNASGYVTARRQATVSAKLTAKVIEVLVEEGM
                     KVREGQVLARLDDTNVRTSLHLAEAQLAAATNALAETRVRIKEAAQELQRQTDLIKNQ
                     IATQANYDHAEAAHLALQARLEQQQAEIAVAAKQVAYWQQQLDDTVIRAPFSGIVTSK
                     NAQPGEMISPISAGGGFTRTGICTIVDMESLEIEIDVNESYINRVHPGQAVEAALDAY
                     RDWKIPSKVIAIIPTADRQKSTVKVRVGMDQLDPRILPEMSVKVAFREVGGTGPLAGR
                     TVTIPQAAVRQQDGRSVVWVVSAGRAERRAVTVSNTHQGETVVSAGLSAGERVIVEGP
                     PDLKEGLAVAEKEAARQ"
     gene            complement(18747..20057)
                     /locus_tag="PKI07_05135"
     CDS             complement(18747..20057)
                     /locus_tag="PKI07_05135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOC50469.1"
                     /translation="MKATLTVRERAEKALAAREGEGAEAGFAGRAMAVFACGGVIRAG
                     RLCGWVCCLGLGLAAAAAQPVPRGYSIPLIDLAGETQRQVIVDREPGQYLGHPTTVLL
                     EDHRALLVVYPKGHGRGAIVYKRSADGGRSWSGRLPVPKSWATSLEVPTLYRTVDADG
                     RKRLIMFSGLYPIRMAVSTDEGGSWSELKPIGNFGGVVAMASCVALKPGPGRYMALFH
                     DDGRFIAGGEEKTSRRKDSPDGPFSPCGKPWQFFVYQCRSTDGGQTWSVPIPIATRPD
                     VDLCEPGAVRSLDGREIAVLLRENKRQRNSFIIFTRDEGLTWTQPRELPGALTGDRHT
                     ACYAPDGRLFISFRDMAHDSPTRGDWVAWVGQYADLVNGAEGQYRVRLMDNTKGADCA
                     YPGVEVLPDGTFVATTYGHWTAGESPYIVCVRLKLEELDARARK"
     gene            20455..21249
                     /locus_tag="PKI07_05140"
     CDS             20455..21249
                     /locus_tag="PKI07_05140"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOC50470.1"
                     /translation="MTQYLDLLRQVLAHGKARTDRTGTGTYSVFGAQARFPLGQGFPL
                     LTTKKVHLKSIIYELLWFLRGDTNVKYLHEHGVTIWDEWADAEGSLGRIYGAQWCAWR
                     TPAGGTVNQIDRVIEQIRTQPDSRRLIVSAWNAGELEQMALAPCHALFQFYVQDGELD
                     CQLYQRSADLFLGVPFNIASYALLTMMVAQVCGLKPGTFIHTFGDLHLYSNHLEQARQ
                     QLRREPRPLPRMRLNPAVRDIHAFQYADFELADYTPHPGIPAPIAV"
     gene            21266..21736
                     /locus_tag="PKI07_05145"
     CDS             21266..21736
                     /locus_tag="PKI07_05145"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOC50471.1"
                     /translation="MPFKAIAAMSLNRVIGAGNRLPWHLPEDFRWFKRMTTGQVIVMG
                     RKTFESIGRPLPHRTTVVLTRAGAPIPGVRVVADLGQLDAADPAFAGREIFICGGAQI
                     YQQALPRCSDLYLTLVLREVEGDTFFPPFEDRFALAGEVLAHPEFRILHYQARV"
     gene            21786..23345
                     /gene="murJ"
                     /locus_tag="PKI07_05150"
     CDS             21786..23345
                     /gene="murJ"
                     /locus_tag="PKI07_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOC50472.1"
                     /translation="MLKSSGAMAAATLTSRVLGMVREMVYAGFMGNTWVASAFTLAFQ
                     VPNLFRRLLGEGALTAAFIPLFKQKELQEGEPEMWRAANAVISGLVAAAAAVAGLVML
                     GITLALAGGSLAVETQLMLRLLRLMFPYMLLVCLAAVFIGMANARGHFFVPALGAVVL
                     NVIMIGSVLLLAPRLGRRLEEQIFGLAIGVLLAGLAQAFFQWPSLAREGYRYRWVSPW
                     RDPTVREVARKMLPGSIGVAAFQINVLITQCFSFWFEPTIVATFNYAVRLMELPQGLF
                     GISLATYLLPTLAGLAAEKKHPEFRQTLGQGLGYLSFANLLAAALALALAAPIVRLIF
                     EHGKFGPDATLRVALALACLAPGLLMFSMNNILARAFYALSDIQTPMKISLVCLGLNL
                     GFAFWLVQSYREAGLAVANTLSATLNLGLLLYALRRKLGRLELAGLNRALGVLVPNAI
                     LAGGVAAALSWLWERHLGHATLGLKLGAVFAPGGVAGLGYWLLALGFKVPAAREMTEL
                     AWQRWRRRPRG"
     gene            complement(23358..24926)
                     /locus_tag="PKI07_05155"
     CDS             complement(23358..24926)
                     /locus_tag="PKI07_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50473.1"
                     /translation="MKTTPTFPTCAGCPLNRRTFLARTGAAAAGALGALAAPAWLDAA
                     PPAAKTRVRVIFALHAEKQPCPDWPNQGFDFKPHMERILNLLTRHCPGFEFIRSTATG
                     EEQARALLQQDADRGGVDGYLVCQMNCWNRVVQTIATSGKPVLYADFQFGGSGGFLVY
                     TAALLRSRTPNVGFVASSRPRDLVDAVKCFRAAGPGGTAADFVAATERARRRGTPRAG
                     RSAGRCDPVPCLAPRECLRQLQAAKILAVRDQSSGPEGEVMGVQVVRVPFAELNEAWK
                     QANPDEARAVAERWMRGAKRIEGVTRAEIENSAAMYLAEKALLQRHGARGITINCLGG
                     FYGNHIHAYPCLGFHQLNNEGLVGGCECDVRSAATMVIGTTLTQGRPGYISDPVIDTS
                     RRRIIYAHCVAPTRVFGPAGKANPYEILTHSEDRQGASIRSLLPTGYLTTSLECDAAR
                     KEILLHRALAVANDPDDRACRTKLCAEPLGDLEKLFRMWDQWSWHRVTFYGDLKEPVY
                     ALAEAAGWKVVEEA"
     gene            25169..26335
                     /locus_tag="PKI07_05160"
     CDS             25169..26335
                     /locus_tag="PKI07_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOC50474.1"
                     /translation="MKTLHAYLMRQMLASLALTVMVFTFVLLLGNALRQILPLLVNRQ
                     MTFGVVAEAAALLIPFAWVFALPMGLLTATLLVFGRFSADQELTAARASGISLLSLIS
                     PVLLLSLALCGLSALFNMEIGPRCRVAYDNILFNLRLELSGVQLPEGRFIKDFPGYTF
                     YVGKNRRGDLRDVMAWVLEHETNVVTYVRAARGRLETDAARKSVTLHLYDGKVVDFRD
                     DQMIPAAFAELPVRLDPKPRRTTYQPKIDNMTATQLWEELREWEARLNAPEPGRRYSA
                     EELRARRRELARRMGDPTTPIRFQIHQQVAFSFACFGFTLVGIPLGIRMHRRETNVGF
                     GVALILVGIYYGFMVLGETFDKQAEYAPHLIVWLPNFIFQAVGAVLLWRANRGI"
     gene            complement(26366..26650)
                     /locus_tag="PKI07_05165"
     CDS             complement(26366..26650)
                     /locus_tag="PKI07_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOC50475.1"
                     /translation="MNEPIPLPIDGVLDLHTFRPREVKDLVPDYLAACRERGIRQVRI
                     IHGKGLGQLKRTMHALLARHPDVVSFSLDYPQFGGWGATLVHLRPPEAQR"
     gene            complement(26714..28018)
                     /locus_tag="PKI07_05170"
     CDS             complement(26714..28018)
                     /locus_tag="PKI07_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50476.1"
                     /translation="MTDSITRRRFLQQTSTLAAGLGALGHAALLRGAGSPNEKVVVAV
                     VGCNGRGMAHIAGFLALPHVEVGYVCDVDSRAVEKGVAAVARKQAKKPLGVKDFRTIL
                     DRPDLDAISIATPEHWHAPATLLACAAGKHVYVEKPGSHNLREGRLMVAAARKYRRVV
                     QMGNQRRSWPWVIESIAALHAGELGKLFFARGWYMNHRPSIGRGRPVPVPDWLDYSLW
                     QGPAPEQPYRDNILHYNWHWFWNWGTGESGNNGVHMLDLARLGLQVDLPTRITCGGAR
                     YFHQDDWETPDTMSATFDFGDKGIAWECQSCAPRGFEGATVGVNFYGENGCLAMAGSN
                     TVIYDLSNKVVREIKSQRAGLFDFDAIHFANFLDAIREGKPLKSDIEDGQKSTALCHL
                     ANIAWRTGHTLNFDPRTQKIVGDKTAAALASRTYRRGWEPKV"
     gene            complement(28096..28428)
                     /locus_tag="PKI07_05175"
     CDS             complement(28096..28428)
                     /locus_tag="PKI07_05175"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HOC50477.1"
                     /translation="MIEDTLSQIEKRIQSAGTIDAGPRQELLQLLGTLKSEITELSRT
                     HGEQAQSIAGFAEVSAHEATRAAQNPELLRLSLDGLGSSVREFERSHPRLVQIVNALS
                     STLANLGI"
     gene            complement(28546..29211)
                     /gene="tmk"
                     /locus_tag="PKI07_05180"
     CDS             complement(28546..29211)
                     /gene="tmk"
                     /locus_tag="PKI07_05180"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOC50478.1"
                     /translation="MPGRLITFEGTEGSGKTTQISLLAERLRAQGHPVRTLREPGGTA
                     IGEEIRHTLKHSPANHAMTPEAELLLMNASRAQLVREVIRPALAAGQIVLCDRYYDST
                     IAYQGYGRQLDLRMVQSLIEVAVGGTRPDLTLVFVVPQELSEARRRVRQPTLPLSLKR
                     DRFEEADRAFFDRVAQGYQALAAAEPGRVRLLDATGKVADIQAAIWDLVQPLLPGKGR
                     PAG"
     gene            complement(29213..30628)
                     /gene="cysS"
                     /locus_tag="PKI07_05185"
     CDS             complement(29213..30628)
                     /gene="cysS"
                     /locus_tag="PKI07_05185"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOC50479.1"
                     /translation="MGLKLFNTLSRSVQEFVPLDPAGRTVGLYACGPTVYDFAHIGNW
                     RTFVFGDLVRRYLDFKGYAVRQVMNITDVDDKIIQRVRETGQPLREFTARYEKAFFDD
                     LEALNCRRPHQTPRATEHIADIIALIERLQARGLAYATPEGSVYFSIEKYRGGGARYG
                     RLVKLNFEAMRVGERVRSDEYEKEAVADFALWKARTPEDGQVFWPSPWGEGRPGWHIE
                     CSAMSMKLLGPSFDLHLGGEDLMFPHHEDEIAQSEGAALQPPGRPFVKYWLHGAHLLV
                     EGRKMSKSLGNFFTLRDLLARGFTGREIRHLLLTAHYRETFNFTLEGLQAARAALARI
                     DECLLKLRAAAGAAPAAGEAPLVEAFTQALDDDLNISGAWGAVFEWVRDTNRKLAENA
                     LDAPAAAAALAAWNRVDSVLGVGAPPEVDVPAEITQLVEARQAARQAKDFKRADALRE
                     ELKARGWTIEDTPRGARAKRL"
     gene            complement(30791..31063)
                     /locus_tag="PKI07_05190"
     CDS             complement(30791..31063)
                     /locus_tag="PKI07_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50480.1"
                     /translation="MANAKKKKPRGQAVMLGLGLDSDGHKRLTTGPNFILAGGSEETH
                     EAMTEKAIKINEKLAARRKRLEEVSREEFEEIAHSVGLQRPRPSQN"
     gene            complement(31115..31804)
                     /locus_tag="PKI07_05195"
     CDS             complement(31115..31804)
                     /locus_tag="PKI07_05195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC50481.1"
                     /translation="MSRRIVLITGATRGLGRAMTDEFIRLGHTVWGCGRSPGDLERLR
                     RQAGPPHDFAAVDIAADEQVRAWADRLLGAHGPPDLVLNNAGVINRNARLWDVPAPEF
                     ARVMEVNLNGTVHVIRHFAPAMIQRRRGVIVNFSSGWGRSADAEVAPYCASKWAVEGL
                     TRALARELPRGLAAVALNPGIINTEMLQSCFGGAAAGYPSPAAWARRAVPFLLELGPR
                     DNGQALTAPGA"
     gene            32229..33416
                     /locus_tag="PKI07_05200"
     CDS             32229..33416
                     /locus_tag="PKI07_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC50482.1"
                     /translation="MNKNLSSKSPGISLGLCALALAAGLTAQGAAPVITNLTVVGGVV
                     QFGVQSELGLTNRIQCCTNLGQPEWEVLTNLVVSESPYWFMDAAAAAVAQRFYRVEAL
                     GSSVSPPPGMVLIPAGSFTMGDCMDPGEGWENELPLHTVYVSAFYMDRYEVTKALWDE
                     VKGWNGGNGYVYENAGSGKAANHPVQMVNWRDCVKWCNARSQKEGLVPCYCNEAGLTT
                     IYKAGTNAPYAKWAANGYRLPTEAEWEKAARGGVSGHRFPWADTDNISHSRANYWAVV
                     NYPYDLSYPAGDHPTFATGGHPYTSPVGYFEANGYGLYDMAGNVGEWCWDWYSDEYYS
                     SSPGTDPRGPASGWDRVGRVGRGGGWCCDAYVCRTAHRGYGNPPDGRSNLMGFRAVRA
                     AGQ"
BASE COUNT         5378 a        10769 c        11559 g         5790 t
ORIGIN      
        1 acaccttgag tggaaaccat gataaccaat ttgctgaact tgacggacac aactttttcg
       61 cggttgcagg catgggggcg ggccgggcca ggtcccggat gggggcgggg cgtttctgcc
      121 cgcgcttcgc gcggcggtgg aaagatgttg ccggcggcgg gggcggccag gttatccgga
      181 agacggttct tgaaatccag ggcagagggg gtagcatcag gtcctgggtt tggggtgcat
      241 atgagcagta atgaacgcat taggccaggg tgggttgccg ctgccgccgg ctttgaggga
      301 ggcaggggat agcatggctt gcaatctggt caccgccgca acgtttcggc agttgcaatc
      361 ccggttgctg gaggatttgc gccgcgccgg ggcggagagc gggtggtcgc cgaagtgggt
      421 ggtggttccg agtgcttccc tggccaatca tttgcgattc gagttaagcc ggggcgcttc
      481 caccacggcg ttggctaatg tgcgggtggt caatgtgccg caatttgccc agcggttggc
      541 gttgagcctt ttgcggaccg cgacgccgcc ctggggtgcg ttgctggatt tgctgctttg
      601 ggggctggtg gaggatttgc cgccccaatc gcccctggcg tcgctccgaa cgatcagcgg
      661 cggcccgggt ctgttgcgga cggcgtttgt ggatttggcc gaggccggat tgggcccggg
      721 cgaaatatgg aagctggagg aattggcggg tcaaccggat atgccgtccc gcgataggga
      781 attgctgttg ctgtatgtgc ggtgggtgca gcttttgcag gagcggcagg tggagtgggc
      841 gcccctggtc ttgcagcggt tgcctggccg cctggaggag gccgaggacc gggagctggc
      901 ggcggcgctg ggcgctgagg cgggccagtc gccgaccctt tatatttatg gcttttacga
      961 ctggattgat gttcacctgg aatggttggg agccctgtcc cgccgggtgg cgaccacggt
     1021 ttattatccg tggatgggcg cgccggaggg agcgcatccg gcctttgagt tcacggggcc
     1081 ggtgcggcag tatttgcgag ggcattttgc ttttgccggc gaggacgcgg tcgccgagcc
     1141 gccggaggtg ttgggccagt tctttctgga gactttcccg gaaggctcga ttggcccgca
     1201 gccggacggc ctgagttatc aacgcgcggc ggggacgcgg gcggaggtta tttcggcggc
     1261 gatccgtatt cgggcctggc tggacgacaa ggacaacccg ctggcgcccg aggagattct
     1321 ggtggttgcg ccccaggcgg acgcttacgc ggacgtgatg caggagatat tcgcggcgtt
     1381 cgccattccg gtgcggatcg cggatgtggt ttgtgggccg actccggaaa gcgcgattct
     1441 gcgcatgctg gcccggattt gggaggagca ggctccggcg gaatgggttt tgggtttatt
     1501 gcgggcgtgt ccggatgcca gggtggcccg aggggtcaat ctggatcagt tcgagggcaa
     1561 ggtgcgggag ttggggatat ggggaggggc agcgtggcgc cgggtgctgg cggacgacga
     1621 gttcccggcg cggggcgaag gttcgaggcg tccgcccgtg caatttaacg cggcggagaa
     1681 ggcgctgatc cgggagatta ttgaatttgt tccggcgggc aacgcggagg cggttccccg
     1741 tattacggca agggacgcgc gggcgatgct ggaccgcctg cagaaggatt ggatgagtga
     1801 tccgacgccg ctggaaccgc ttgtccgcgc aacggaggag cttgcgcggt ggcggccgtc
     1861 gctggccatc gagcggcgcc agtggacgca actggtcgcg gagagcgacg gggtccggcg
     1921 gcagcgggat ccgcggtcgc gggcggtgtt gtttttgccg atgatgcggg cgcggggtct
     1981 gaccgcgcgc gggattgtca ttctgggcct ggccgcggga cagattcctt ttcgggtgcc
     2041 ggaggatccg ctgctatcgg agctggcgtc cgcccggctg gcggagtggg ccaaccagat
     2101 tggccatcgc ctgccgccga aggcgcgcct gacggaggag ttggtgctgc ttttcagcct
     2161 gatcaacacc gcgggtcagc gggtccactg ggtcgtgccg gaaaccgatg ccgcggggaa
     2221 agccgtcgct cccacgccct gggttcaacg ctactttaac cgctgggaaa accaggagcc
     2281 gaaagtggcg gaccctttga gccgccggat tgcgcgcgcc ccgtttgagc aggcgattta
     2341 cctgaagaag ttggatccga agcgcggcga gtttttgccg ccggccctgg cgttgtttgt
     2401 gaatcccaag ctggccgccc ggggagaccc ggatgcggcg cgctcctggc ttggcgaatc
     2461 catcgcccgg cggggcaaaa agccggagtg gagcggaagc atccagtgtc agccggattt
     2521 ccggaacggc cgggtgaatg ttaccagcct tgaggcgctg gcacaatgtc cgttccgttt
     2581 ctacggggaa caggtggccg gctggaaggc gctggaggcg ctttcgttga gccatgaatt
     2641 ggcggcactg gcccgcggta gcttgctgca taagctcctg gaagaggcgg tgcggccgca
     2701 tttgcggacg agccgggtcg ccgaaatcgc cgcggagatg ctggcgcagg aatgcgcgaa
     2761 attgcatacg ctggcgcaaa ggctgcccga attttcgccc gcgaccgctg cggcgctggc
     2821 cctgctgccg gaggtgttcc agcaagccgc ccggcggcag atggtgaggc tggcagcggc
     2881 ttactttgag gcggccttga atcatccggc ggtgccgcag gacgtcgagg tggcggtggc
     2941 aaaggagttt ccaggcatcc cgggactgat ggtggcgggg aagatagacc gggttgatca
     3001 caatgaggcg cgggtggaac tgctggatta caagagcggc aaaatgccct ccgactatcg
     3061 gaaaagggtg aagctggggt ggcagattca ggcggtgctt tacccgtggc tcagcgggca
     3121 gaacgacgcc tgtttccgtt atcttttcct gggccgggcg gcggcggagg agggggatgc
     3181 caagggcgcg ccggaggcgg ccggtttcct gagtgaattg gctcccctgt tgcaagcggg
     3241 gcattttatt ccgacatcca atcaagccat ggaagaactc gggctcagca aggtttctcc
     3301 gtgccggtat tgcgccctgg cttcggcgtg ccgccgtttc gagccggggg cggcggccaa
     3361 acacgcccgg ttgtttgaga agcttgcgcc cgcgcgctgc gcgtctttgc ggcaggcggt
     3421 gaatggtaaa cccccggaga aaacggattg atgatacccg atgccagaca gcgccgcgag
     3481 gccgtgctaa cctccgggca tgcgtttgtg tgggccagcg ccgggaccgg caagacccat
     3541 acgctgacgc tgcgcgccct gtatttgctg ttaaccgcgc cgttcgactc gcgcgcccag
     3601 gggacggaat gccgcctgct ctacggtggc gccagccggg cggaacgatt gcgcgccgcg
     3661 cgcgcggtgc tccggcggtt tgtgctgacc acctttacgc gcaaagcggc cgccgagatg
     3721 cagacccgcc tgtacggcta cctggacgcc ctggccagcg cggccagcct caccgcgctg
     3781 cgcgagcgct ttcccaacga caggcagttg cgggaagtgg tcgaggcgac gttaggccgg
     3841 ctgggcggcg gcgagccttt ttcccggctg caagcgggcg cggaagcgct gggagaactg
     3901 gcgacggaac tgcgggtatg cacgctgcac agctttgcgg cgagcctgct gcagcggcat
     3961 tccattgccg ccggcatccc gcccctggcg caatttgccg aggaagatga tccgacgctg
     4021 atgaatgtgg gcagccaggt ggtcgagcgt tggtggcaaa aggtgctggc ggaacccgaa
     4081 ttgctccagg acctggaggc gttgatcccg attctgcccc aggaagacat gcgggaatgg
     4141 tttgaggcga tctatcagca tcggcggatc gccgacgaga tggattttcc cgcacccgac
     4201 aaccacgcca tccacgcgct gctggcagca agccgccaac tcgcccagga gttggcgggc
     4261 gcgccttccc gcgccaccag ggtgatccag gccggcaggg aactgcaggc gatcctggcg
     4321 agcattagca acggccaggc cttcgcctgg cgggatttct gcggctacct ggagagacaa
     4381 tccgaggtgc tcttcagctc gaagaggtcg caggcggtcc agaaggcaat cgccgcgctg
     4441 ggcgccggaa gccggtttta tgaagacgac ggcttaatct atgggctcgc ccttcagaaa
     4501 tgccttgcgg aagagtaccc ggaggattgg gcgcggtggc ggcgcctggc tggccgcttt
     4561 gccaagtggg cggaggatgc ggccgtgcgc gaactgggcg tcgtgacctt cgacgatatg
     4621 gtgcgcctgg ccgcgcggct gctgcgcgaa aacgatgccg tgcgccggga ggagcgggag
     4681 cagctttggg cgatcctggt ggatgagttt caggatacgg atcccctgca gttggacctg
     4741 ctggagagcc tgctgcgccg ccagccgggc gaccctgagt tgatgggctt tttcgtgggg
     4801 gatctcaagc agtccatcta tcggtttcgc ggcgcggacc tgccggcgat ccgggggttt
     4861 gtgagccgct attcgcaggt ggttcaaagt accgaggtgg gagagtttca gcttacggcc
     4921 aactttcgga gccgcccgcc aatcacgaag ttcgtgaatt attttttcga gcggtgcgtg
     4981 ccgcttccta attacccaaa cgagcggctg gaaccggtgc ggaagggagc cacccccatg
     5041 cccgagtggc gccggcttgc tttggcgcag cagcgtggcg cggttggcgc gcggcttgag
     5101 tccgcgggcc gggaaaccgt gcggttgatc gcagagcacc acgaccagtc cggccaagat
     5161 gacgcggctt accgcgatat tatcgtgctg gtgagcacgc atgacgaact ggatatgctg
     5221 ttgccgttgc tggagaaggc gggcattccg gcggtcagcg gcggcgcgag aactttttgc
     5281 caacaaccgg aggtgctgga cgtgttgaat ctgctcattg cggcgcagaa tccgctggac
     5341 accctcgcga ccggggcttt gttgcgcagc ccgcttttcg ggctgtcgga tgttcaaatt
     5401 tacgggctgt tggcgcgcct ggagccgggc gagatatttc atggcgacgc ggcgctgccc
     5461 gcggacctgc cggcgcccgc ccgcggccgg atcgagcaac tgcggcgcct ggtgcgggag
     5521 cggcccggca ccgcgctggc gatgtggcta agccagttgc gggagttcat tcccatgggg
     5581 ttgtatgcgc aacagaatat agaagggcgc ggggttgcgc gaattgacca ggtgctggcc
     5641 gctttccgcc gcgccgtcga attgggttcg ctcccgccga taagctggct gctggaacag
     5701 cgggaccggg cccgggatcg gtttgacgcc aaaccgggag aggatgtgag cgtgacggac
     5761 gaaagcacgg cggcggtgcg cgtgctgacg attcacaaag ccaaaggatt gcagggcaag
     5821 tatgtgatcg tcctgggctg gcagaaggcg ctggacaagt tgaagcccag ggaggaatcg
     5881 agctgccatt ttcagcttac cgatgcggac ggccatacgg ttggcggatt caaattgccg
     5941 tggggacggc tggaggtggt ttcgaccgac tatcgcaaag cccagaaact gaatcagcaa
     6001 tatgaagcgg aagaagccca gcgcctgctc tacgtggccg ccacccgcgc ggaggatcgc
     6061 ctggtgctgt tgtcccctca ttgccaggca atcccccaag gtcaactggt ggaaatcccg
     6121 tccccgctgc aacaagacgg gggtgcggga gaagcatgcg gcggcacgct ggaggtaatt
     6181 gtaacgctcc cgccggagcc gatccagcgc ccgtcgggca gcgcagtgcg cctccgcagt
     6241 cctgatgcct acgcgcaact gtggcgcgcc cggcttgagc gcgcggccgg agaagcggag
     6301 cctttgctgc atcaaccctc gcgcctcgaa ccggtggaag cggaggacaa agctgatttg
     6361 ccggactatg tgcgggaaaa gattgatatg gcatcggccc gggcgatgga actgggaaca
     6421 gtggtgcatc gttacctgga gcggcacttg caggaacccg gatacgacgc cgcgaagctg
     6481 gaggcggtgg tctcggaaat caacgggggc gtaagtttcc ctgaaaccat cgaaaaggcg
     6541 cgcgttgttc ttacggattt ctacggcggc gcgcttcatc agcgcgcctg ccgcggccgg
     6601 atcgaagcgc gggaggcccc ggtctttgca agctgggaag gcaaagcctg gagcggcgtg
     6661 attgacctcg tcctgcgcga gggggacgcc gtaattggcg tggactacaa agtcatgaaa
     6721 atgccgaagc aactcccggc ggaatacgaa caacaacgcc gggtttacca ggaggttctc
     6781 cggcgcctct tcccggggca accggtgtcg ttcgagttct ggtggttaat taactcccca
     6841 cggtgaccct ggtgtttccg gttttctccc gcgcaacgac cacaaccacc tttgaaacgg
     6901 cttcgcctcc gggccgcgcg ggtttttgca gatcatcctg ctccatgttt ccccgccggt
     6961 tgcgggccca ccggctccgg cccgatcccg cgcatgcccg ttcatggaga ttcggaatca
     7021 cgaggcaatc aacttgaccc gatccccgct ccgggttata cccattaagc ccgcgagctt
     7081 gcggtttgat atggcctgct gccaggcggc aaaggcagga cgcgccatcc ggcgcggtca
     7141 aattggaaac tccgaaggcg cgacctccgc cgcaatcatt gcgcgtcccg ggccagttta
     7201 gctcagtggt agagcaacgg ttttgtaaac cgtcggtcct cggttcgaat ccgagaactg
     7261 gctccatcgc cgcaaaacgg ctccgccccg ggcgggtcag gccggcgcgg ctttggcgta
     7321 ggctaacggc accaccagca ccgcacactc cgccttgcgc acgacttttt ccgccacgct
     7381 gccaaaaagc gccttgccca tgccgccgtg gccgtgccgg ccaaggacaa tgacgtcaat
     7441 ccgctcggac cgggcgaact caagaatcgt cgccgcatcc ggcccgatgc ggaagaccgt
     7501 ctgcaggttc atgcccggag gcacgcgcgc gagataatcc gccgcgatct tcgcatcaat
     7561 ggtctttttt gcctcggcgt tgatgttttc cacctcggcg agctgcgttt tccaatactg
     7621 cgcatccggc tcgtgaatga cgtgcatgag atacagcgtc gcatccggcc ggcgggcggc
     7681 ggcctccagc gcgaacggga acgcggccgc ggcgctttcg gagaagtcgg tgcaaaagag
     7741 gatgcgctga aagcgcgaag cgggaagcgg ggaagagctc atagggggac ggctatttaa
     7801 tcaaaccggg caggaaagtg gcaatttgcg ggaaaaggat gagcaggatt gccgccagca
     7861 ccagcgcccc caggaagggc atgctgccgc ggaagatttt ttcaatgggc acatcccggg
     7921 acatgcccgc gacgacatac acgcaaacgc cgaccggggg agtgatgacg cccatcatgg
     7981 tcaccaccac aatcatgacg ccaaaccaga tgatgtcata gcccagcgcc aggatcagcg
     8041 gatagaagat tggaatggtc aggatgtcca aggccagcgc gtccagaaag cacccgccca
     8101 ccagataaaa cagcataatc aagccaatga tgccccaacc gggcagcggc aatcccgaca
     8161 gccacgcggc cagggtggag ggcacctggg tcacggccag aaacttcccg aaaataatgg
     8221 ccccggcaac gatgaccatc accatgcatg ccgtgcggat ggattcgttc aggcactggg
     8281 tgaacttgcg ccaggtcagc tcgcgcttga gcagggcgat gaccagcgtg cccgccgcgc
     8341 cgacggccgc gccttcggtg gcggtgaaga agccggcgaa catcccgccc atgaccagca
     8401 cgaacagcgt caacgtttcc catacgccct tgagcgaggc gaaccgttcg cgccaggaga
     8461 agaccggccc cggcggtccg agatgcggat ggcgtttgca ttcgaagtag atgacggccc
     8521 agaacaacag cgcgatcatc agcccggggg ccacgccggc gataaagagt ttgccgatgg
     8581 attgctcggt caggatggca tagacgatga acacgatgct gggcgggatc agcatgccga
     8641 tgccgccgcc cgccgccacg cagccggtgg ccagttgcat gtcgtaccgg taacgccgca
     8701 tttcgggcag ggcgacggac gcgatggtgg ccgaggtggc ggggcccgag ccgcagatgg
     8761 cgccaaaggc ggtgcaggcg ccgatcgtgg acatggccag ccctcccggc agccgtccca
     8821 tccagtggta ggcggcatgg aacaatccgc ggctgatgcc ggtgtggaag gcgatctgcc
     8881 ccatcaggac aaacaaggga atggtggtga ggttgtaatc ggagaaagtg gtgtagaggt
     8941 caatcgtggc catgctgaag gccgccgccg gcgacaccat gacggcaaag cccaccaggc
     9001 ccaccgccgc catgacgtag cccaccggca tggaccccgc caggaggagc agcagcaaag
     9061 caaagccgaa aagggcgcct tcgttcgggc tcatggcttg atcatcggct tgccgggatg
     9121 cgccaggtga tacgccttga tcagcaccac caacccgcac gaaaccgcca gcacatacgg
     9181 cacccagaag atcggcagtt gcagcgtcat gctgacctcc ccccgggtcc ggagcgaatg
     9241 gccatagtcc acgcatcccc aggccagcag gctgaatagc gccatgcccg ccagccgcat
     9301 gagggcgtcc accacaatgc gcccgcgccg ccccagttta tggaagaaat actcgatggc
     9361 gacgtggccc ttgatggcgg tggtgtaggg cagcgccgcg gcaatggtaa tcgcggcggc
     9421 aatcttgaca atgtcatacg cgccggtcag cgacaggcgg aagaggcgca gcaccacttc
     9481 cgcgctggtg accagcacca tgaccatgag gctggcgcac gccacatagg ccagcgccat
     9541 caccagcgcc cgcagcgcct ggtcataaag gcgccacggg ctttgcggac cggacggagg
     9601 aacgggcggc gaactcatcg ggcggccgtt cccgggctag tgccgccgga atatccgccg
     9661 ccgcggcgcg ccagacgggc cggtttcgtt ggctggtatt gcgaaatcat cccgcttcgg
     9721 cggggctgcc accttttcgg gttactggct ggttttggac gcttgaatgc ccgcttgaat
     9781 atccgccagc atcgcctcgc cgggcaggtt cttttctttg gcctgcttcg cgaaatcatc
     9841 caggatcggc ttgacggccg ccttccagcg ttgctgctcg gcttcgggca gatgaatgaa
     9901 ctggcgcttg aggtcggtga cgaattcgcg tccggcttga tccgcctcat cccaggcggc
     9961 gccgtgtttg gcgatccatt cctggcttac ctcggtgaag acctgctgca cgtcggccgg
    10021 cagcgccgcc catttatcct tgttcatcac cacgaacatg gccgtcgtgt agccaatggc
    10081 cgtggcgtcc accacgtatt cgatggtctc cccctgtttc cagcccttga gcgtttcgat
    10141 ggggcacagc gtggcttcca caacgccttt ggccagggct tcgtaggttt ccggctggct
    10201 catggcgacc ggcgtggcgc ccagcgcctc gacaatcttc gccgacaatc cggtggcgcg
    10261 cactttcatg cccttcagct catccagcga atgcaccggc ttcttgctcg cgaggatgcc
    10321 cggcccgtgc gcgtggacgt aaagcatttt cacgtcgttc agttcggcgg gctggtattt
    10381 ttggatgatg gtgttggcga ggcgcgtggc ggtggcgcca tcgggatacc ccaacggcag
    10441 gtccagtccc tcgagcaacg ggaaccgccc gcgggtgtag gcaaaacagc tcatcccgag
    10501 gtccgaaatc cctttaatca ccccctcgta gcactggtcg gctttggtaa gggaaccggc
    10561 gggataaacc gtgatctgca cgcgcccgcc ggtgcgctgc tggatttcat gcgcccagtt
    10621 ggtggcggcg atgcactgca catgcgtcgg ggggaagaag acgctatagg agagcttgat
    10681 cgtttccttt ggttcggccg gtttgcaagc ggtcagacaa agggccgcga ggcccgcggc
    10741 gcccgccatt agtacccatg gtttcatgct cataagaact gtgtttcagg tttcatcatg
    10801 tttctgcgcc ccgcaccggc gcggtcgcgc gcaagccggg cgcccgtcac ggccagttta
    10861 taactccgtt cccccgccgg ccgcaacact tcttcttgcc tcgccggcct cagcccacct
    10921 ggaaggaaat tcgccgcacc aggtcccggc caaagctgtg ctgcaaccgc tccaggatct
    10981 ccttgcggtg atagcgcacg atttcgctca gccagacgct gctatccacc gtgacgtaga
    11041 gcgtgccctt gttcagcccg gtcggctggg catgcgcggt gagattcgga tccagcaaat
    11101 cattccacgc tttggcgatc tccgcctcgc tccggcggcg atccaggcgg agcgtcgcaa
    11161 gaacgcgcgt caaaacggtc cccaccggct gggcgcgcag ggcttgcgcc tgttccagcg
    11221 gggccaggtc aaccccgcgc cactgcgcgc agacgcgctc gcggggagtc tgcggggcgg
    11281 ggccgtgcgg cggcgggcgc atcttatccg tgccggccgc gccggggttg tcgttccggg
    11341 ctcattcggt ttcgccggcg ggtttcgcct ccagcggttc atccagcacg agcactccgc
    11401 ggggcggcag gtcgtagttg ccggagatcg cgttgccggt cagggaatcg tgcatcggct
    11461 tgtagaactg gacgcgcacc ggcgagttct gatgattcag caggaagtag aggttggcgt
    11521 cgtccttctg gcgctggcat agctcgatat tatccggcac gcgcagcggt ggatgcaggt
    11581 tgcacatctg gcgcagccag gtcaccaggt cggtgtagaa atgcgcgtgg ctctgcgtgc
    11641 cgatgtaaat ggccttgccc aggccgaacg gatgcagcgt catcgccggg cggccggcgt
    11701 agaaatcctt ggcgtagctg gcgatcacct ggcaatcgcc cggctcgatg aggtcgcacc
    11761 acagccgggc cgggtgcatg tggctggccg ggaaggcccc cttgaacgtg agatgattct
    11821 cctcccccgg cggcaagtga tcgaactcga ccacctccag gccgaacaag tcggtcaggt
    11881 cgtgcgggta gccggtgtcg ggcacctggt ggtattcatc caccaacccg gtgttgaacg
    11941 tggcgaccag cgtgccgccg ttttggacgt agagcttcag ccggtcggct tccccgccgg
    12001 ccagcaaatg cagggacggc gccagcacca gcttgtagcg cgagaggtct tcggtggggc
    12061 gggcaaaatc catcgcaaga ttccgctcat gcagcgcgtt gtaaaagagc tggatatgct
    12121 cccgcaggtt gaaaaactgg ttgcggtgcg tcgcctgctg cagcgcccat tcattgtcgt
    12181 gggtgtacag gaggcaaacc tccgccgcca cgcgcgaatg cttgagcgtc ggggacagca
    12241 gcttcatttc gtcgcccaac tggtccactt ccttgaacac ccgcgcggtg gcgcgcgccc
    12301 ggtgcggcat caccgccccg tggaattgct cgcccccgaa acgcggctgg cgccaccgga
    12361 aaaagacaat cgcctccgcc ccccgcgcca tcaactgata gctaaacagc cgcagcatgc
    12421 cgggccgcat cagcgcgtgg acctcctgcc agttcacgct gcccgccttc tgctccatca
    12481 cccagaaacc ggcgtcgccg tccggggtcc ggatgtccgc cttcttgagc gagcgcagca
    12541 tgtcaatttc gcaggccatg cccgacggtt tgggcttgag gacggcgtgg ctctcgatgg
    12601 agacaaaatc aatcacctcc gccaggtcga agtggtcaaa gcggtgcagc aacggccgca
    12661 ggttcgtggt gaccgggcat tgcggcgtga gctgatgcag caaatcggcc tgcatcttga
    12721 caaagcccac gatgatgtcg ctgcagaacc ggcgccaatc cagcaccagc gccgggttgt
    12781 gcgcggtcgg cgcccgcatc ggcatgggca cctgcttcca gctcgtgacg ttttggcccc
    12841 agtgccgcaa ccccatcagg ttgttcagcc gctcgatggt ttcatacttg ttctccagcc
    12901 acaggtgcca ctcccggcgc gcgtcttcat tgaacgacgc ctcggtgaaa ttgccgccca
    12961 cgccgttgtc aatctgccag gcgatgagct gcggatgcgg gccgagcgcc ttggccatct
    13021 gctccacaat gcggcgggaa tattcccaat acttgtcgct ggccaggcat acggcgtggc
    13081 gggtgccctc ccgcttcacc agcccccgct cgtccaccgg caggatgtcc gggtgtttct
    13141 gggtgagcca gatgggcggc gcggcggtgg gcgtggccag caccaccttg atgcccgcct
    13201 tgcccagcaa atccatcacc cggcccagcc agccaaagtc aaactgcccc tcctccggct
    13261 cgcacaaccc ccaggcgaac tcgcccatgc gcaccacatt gatgccggcg gactgcatcc
    13321 actcgatgtc ctgctcccag gccgcctccg gacgctccgg cgtcccgccg tacgggtaaa
    13381 cccactgctc cggatgataa cttacgccgt aatacataat gcttcctcgc gttgcgaccg
    13441 acaccctact ttagcgggaa tccgcccccg gcgcaatgca aaagtggccc gaccggccac
    13501 ccgcaaagac cgcttgacac ccgctccaaa cccgcccgcg cccggcggcg ccggcctcag
    13561 cgcgcccgcc cgagattctg cagaatccgg accgtggccg ccgccttgtt cagggtgtag
    13621 aaatgaatcc cgggcacgtc cgcctggagc aacccctcgc attggcgggt ggcatactcg
    13681 atgccaaact cggcgaccgc cgcgtcgtcc ccgcccagtt ccgccagccg cgccgccagg
    13741 ggcgcgggga tgctggcgcc gcacatggcg gtaaatcgct ggatttgggc gccgctcaaa
    13801 atggatacca cccccggcac aatcgggacc tggaccccca actcgcgggt cagatgatcg
    13861 cggaagcaga aaaaatcagc gttgtcaaag aacaactggg taaggacaaa gtccgcgccc
    13921 gcatcaatct tctccttgag atggcgccaa tcggcgtgcc ggcccgcgcg gcaggccgtg
    13981 tggccttcgg ggaaacccgc cacgccaatg ctgaagtcgc cctgcgcgcg aatgaactgc
    14041 accaactggc gggcaaactc aaagcccccc ggcgccggct gaaactcgcc gccgccgggc
    14101 ggatcgccgc ggagggccag gaggttccgg atgcccagcg cgcgcagttg ggccagcagc
    14161 tcgccgatct gctcccgcgt ggcgcacaca caggtcaggt gcgccaccgt ggtcagcgcg
    14221 tgctgccgct ggatgcgctc cacgatccgg agcgttttgt cccgcgtgct gccgcccgcc
    14281 ccgtacgtga ccgagcaata atccggacgg gcctgcaaaa gcgcgggcag cgtcttctcg
    14341 aacaggatgc ggtcgccctc gtcggttttc ggcggaaaga actcgaacga aaacaccggc
    14401 ctccccgcgg tgcgtccgtt cgcatagatg tcacgaatga actgcatcaa ccgccagcgt
    14461 gcgccatccg caaccaaaaa ccaagcccga atccccaagg agcagcgctt cataatcccc
    14521 agcccccggc gcgcgggcct ccggcccgct ttgggcggtc tttctttcgg gcgcctgctt
    14581 cagtttccac ggcagtctgc cgtcccgtac ttaaagcgga caagatgtcc gcgcgccggc
    14641 gttggcagtc ggtatatcca gcgcataaag cccgggattg tacggcacca cgccccgcgg
    14701 ctcggcgggg ttgccgggat tcttctgctc gggcatggga caatgatgag ggaccaggcc
    14761 ggcaaagtcc actcccagca gccttcgaat catcccccgc ccggcgcgcg ggcctccggc
    14821 ccgctttggg cggtcttttt ttcgagcgcc tgcttcagtt tccacggcag tctgccgtcc
    14881 catgcccaaa gcggacaaga tgtccgcgcg ccggcgttgg cagccggtat aaccattggg
    14941 ttggaatccg cccggcctgg gttgttcgga tagtgtaagc gggccggagg cccgcgcgcc
    15001 gggcggggtt taggatgagg ggcggcgcgt gaaggcgccg gaacgccatt ccccctgggt
    15061 gcgcgcggcg atctgccgca gcccggcggc ggcgaacgcg cgccggactt gctcatactc
    15121 cgccgccagg atgcccgccg ccaccagcac gccgtccggc cgcagccgcg cgagcaaacg
    15181 ctcccgctgc tcccgcagca ggcccgccac caggttggcg cagacgatgg cgtattgcct
    15241 ccgggcgcgc cgcggcagcc gcgtcaaatc ccgccgtgca aaaccaatgc gcccggccac
    15301 ccggttgcgc cgggcgttgg cccgcgccac cgccaccgcc tggggatcat catcaaacgc
    15361 gtccaccggc gcataaccga gccgggcggc ggcgatggcg agaatgcccg agccggtgcc
    15421 gatatccagg caggactgcg cctcgcccgg ccggcggcgc gccaccagtt gccgcaggca
    15481 gaacgcggtc gtcggatgcc gccccgtgcc gaagctcaat cccggatcga gcaccaccac
    15541 cgcctggcct ttccggggcc gccgccggct ccagcccggc ttgagcagca gggccgaccc
    15601 gatctccagc ggcgggaagt gccgcttcca cgattccgcc caatccgccc ggcgcagccg
    15661 ccgcagcgag attcgccccg gcccgaggtc aagcccgctg gccgctatcc ggcgcaagcc
    15721 ccgcgccagc gccgaagggc aaaccccgcc gcgccacccg gccggctttt cctccagata
    15781 aaccgagacc accgtttccc gggtttcggc gtccgtgtag gaagaggcgg gcggccccag
    15841 cacgctcccc agcaactcgg ccaccgcctc ctccgcctcc ggcgtcgtcc ggacggagac
    15901 ctgccaaagc cgggccggtt tcatcgggcc cggcccgcgg cccagccggg cgttgccatt
    15961 ggaaatcccg cagcgttcac ccccacagcc tggcagaacc gggcccgccg cgcaatcccg
    16021 cccgggcgcg gcttacgcgc cgggttcccg ccagggcgcg tagttgatca gttgcagttg
    16081 gacgcgctcc gggtgcagcc ggacgcgggt gacgctggca taggcgatct cgaacgcccc
    16141 caggcgcgcg agcggccagt gcagcaaaag ggccagcacc acgcggataa tgccgccgtg
    16201 gcagaagacc gccacctgcc ggccgggctg ctcgtcctgg atgcgccgca ggcacggcgc
    16261 gacccgttcc cgcagggcgg caaatccctc cgcccgggcg atgcggttgt tttcgatttg
    16321 ctccagccag ctccaggcgc tgacgccaaa ccgggcctgg acggcctcgc cgctcagccc
    16381 ggtccaatcg ccaaaatcca tctcgcgcaa gtccggcagg atgaccggcg gcggcgcgcc
    16441 gcgggccagc aggggcgcga gggtttgctg cacgcgcttc atcgggctgg cgtaaatcgc
    16501 gtccagcggc tggcggcgca gataatccgc cagcacggcg gcctgctcgt ggccccgcgg
    16561 cgaaagcgcc aggtccatcc ggccgccaaa gatgttctgg ccatcggctt cgacttccgc
    16621 atgacggacc agcagcaggc ggagggcggc ggcggcggcg ggcttcatgg cggcgggcgg
    16681 ggcgcggtca ggtgacgggc tgatcgcaaa tccggccttt atcgagatgc accgtgcgcg
    16741 aggcgcgggc cgaggcatgc gggtcgtgcg tgaccatgat gatcgtcttg ccatgctcgc
    16801 ggttcagccc ctggagcagg tcgaggattt cgtccccggc cttgcggtcc aggtcgccgg
    16861 tgggctcgtc gcacagcagc agggtgggat cggtgacgat ggcgcgggcg atgcccacgc
    16921 gctgctgttc gccgccggag agctggcgcg ggtaatggcg cgcgcgcgcc gccaggccga
    16981 caatcgccag cgcggtggcc acgtgccgtt tccgctgggc gcgggtcagg tgcgtcagca
    17041 gcagcggcag ctcgacgttg cgctccgccg tgagcaccgg cagcaggttg tacaactgga
    17101 aaacaaaacc gatatgccgc gcgcgccacg ccgccagccg gtggtcggac aaccggtcca
    17161 cgcgctcgcc ggcgatgcgg accgtgccgc gcgtgggccg gtccagcccg ccgatcaggt
    17221 tcagcagggt gcttttgccc gagccggacg gccccatcag cgccagaaac tcgcccgccg
    17281 gcacgcgcaa atgcaggtcc tgcaggacgt ggatgtcctc cgcgccgcgg cggaaaacct
    17341 tttccacccc gtccacttcc accaggaccg aatccgcgga cgccgtcatg cctcactggc
    17401 gcgcggcttc cttctcggcc accgccaggc cctccttcaa gtccggcggc ccctccacga
    17461 tgacccgttc ccccgccgag aggccggcgc tgaccaccgt ctcgccctga tgcgtgttgc
    17521 tcaccgtcac ggcccggcgc tcggcgcggc cggcggacac cacccacacc acgctgcgcc
    17581 cgtcctgctg ccgcacggcg gcctggggaa tcgtcaccgt gcgcccggcc agcggcccgg
    17641 tcccgcccac ctcgcggaag gcgaccttca cgctcatctc cggcaggatg cgcgggtcga
    17701 gctggtccat gccgacccgc actttcaccg tggacttctg ccggtcggcg gtggggatga
    17761 tggcaatgac cttcgaggga atcttccagt cccggtaggc gtccagcgcc gcctcgaccg
    17821 cctgcccggg gtgcacccgg ttgatgtagc tttcgttgac atcaatctcg atctccagcg
    17881 attccatgtc cacaatcgtg cagatcccgg tgcgcgtgaa cccgccgccg gcggagatgg
    17941 gcgagatcat ctcgcccggc tgcgcgtttt tggaggtgac aatgccgcta aacggcgcgc
    18001 ggatgaccgt gtcatccaac tgctgctgcc agtaggccac ctgctttgcc gccacggcga
    18061 tctcggcctg ctgctgttcc agccgggcct gcagggccag gtgcgccgcc tcggcatggt
    18121 cgtagttggc ctgggtggca atctggttct tgatcaaatc ggtctgccgc tggagttcct
    18181 gcgcggcttc cttgatgcgc acccgcgttt ccgccagggc gttggtggcc gcggccagct
    18241 gcgcctccgc caggtgcagg ctggtccgca cattcgtatc gtccagccgc gccagaacct
    18301 gcccctcccg cactttcatc ccctcctcga ccagcacctc gatcaccttg gcggtgagct
    18361 tggccgagac cgtcgcctgc cgccgcgcgg tgacgtagcc cgacgcgttc aacaccgtcc
    18421 gctcgacgcc gtcgtcggcg gccgcgcgcg cgaccgccgt gcgcaccggc cgggcggcgg
    18481 gccgctgcag ccagaaaacc acgcccccgg ccaggagcag cgccagcaaa acgcccgccg
    18541 cgccccagcg ccaggacgaa gccgccggcg gcgcgtcgcg ctcgatgcgc aggtcactca
    18601 gcgtgggttt gggctcactc ataagcgatc acgggccaca gcaaaccgtc ttttcccgtc
    18661 cgggtcaatt atggagtgac gcccggcgcg gatgccgagc cggcccgggc cgccgggcgt
    18721 tcccgcgccc gcccgccgcc gcggcgctat ttccgggccc gggcgtccag ctcctccagc
    18781 ttcagccgca cgcagacaat gtaaggcgat tcccccgccg tccaatggcc gtaggtggtg
    18841 gccacgaacg tgccgtcggg caaaacctcc acccccgggt aggcgcagtc ggcgcctttg
    18901 gtgttgtcca tcagccgcac gcggtactgc ccttccgcgc cgttcacgag gtcggcgtat
    18961 tggccgaccc acgccaccca gtcgccgcgc gtgggcgagt cgtgcgccat atcgcgaaag
    19021 gagatgaaca gccggccatc gggcgcgtag caggccgtgt ggcggtcgcc ggtcagcgcg
    19081 cccggcagtt cccgcggctg cgtccaggtc agcccctcat cccgcgtgaa gatgataaag
    19141 ctgttgcgct ggcgtttgtt ctcgcgcagc agaacggcga tctcccggcc gtccagcgaa
    19201 cgcaccgccc ccggttcgca caagtccacg tccggccgcg tggcgatggg aatgggcacg
    19261 ctccaggtct ggccgccatc cgtgctgcgg cattgataca caaagaactg ccagggcttg
    19321 ccgcacggcg aaaaggggcc gtccggcgaa tccttgcggc gggacgtttt ttcctccccg
    19381 cccgcgatga aacggccatc gtcatggaac agcgccatgt agcggcccgg gccgggcttg
    19441 agcgccacgc aggaagccat cgccaccacg ccgccgaaat tgccgatggg cttcagctcg
    19501 ctccagctcc cgccctcgtc cgtgctgacc gccatccgga tgggatacag cccggagaac
    19561 ataataagcc gcttccggcc gtccgcatcc acggtgcggt aaagggtggg cacctcgagc
    19621 gaagtggccc agctcttcgg cacgggcagc cgcccgctcc agctccgccc cccgtccgcg
    19681 ctgcgcttat agacgatcgc cccgcgccca tgccccttgg gatagacgac gagcagggcg
    19741 cgatgatcct ccagcaggac ggtggtcggg tgccccaggt attgccccgg ctcccgatcc
    19801 acaatgacct gccgctgcgt ctcccccgcc aaatcaatca gcggaatgga ataaccgcgt
    19861 ggcaccggct gggccgccgc ggcggccagg cccaggccca ggcagcagac ccacccgcac
    19921 aaccggccgg cccgaataac gccgccgcag gcaaaaaccg ccatcgcccg ccccgcgaac
    19981 cccgcctccg ccccctcgcc ctcccgcgcc gccaacgctt tttcagcccg ctcacgaacc
    20041 gtcagggtag ctttcatatt gcaggcccaa actaccgacc gccacccaaa ggtcaacacc
    20101 atcccggact gtccaatttt tggacactcc tcaccctccc catttgccgc tcatttccgc
    20161 ctctcagccg gcatttcccg aaaacccact caaccaccgt cctttcaagc tcttccatag
    20221 ctcccaccga gccgtgtaca aggggtgtac aagggatgct catcgggtgt accagctaaa
    20281 atcccatgag catccggtga gcatcccttg tacacccctt gtacacggcc cgatgggagg
    20341 gggggaggat ggggggaggc gggagcggag ggggggcggt tggggatgta ttttgggggc
    20401 gggcgcaggt tgctcccgcc ggggcaactc gttagactgg gggctggcat agttatgact
    20461 cagtatcttg atttgctgcg gcaggtgctg gcgcacggga aggcccggac ggaccgcacg
    20521 ggcaccggga cttactcggt gttcggcgcc caggcccgct ttccgctggg gcaggggttt
    20581 ccgctcctga ccaccaagaa ggtgcatctc aagtccatca tctacgaact gctttggttt
    20641 ctgcggggcg atacgaatgt taaatatctg cacgagcatg gggtgacgat ctgggacgag
    20701 tgggcggatg cggagggcag tctcgggcgg atctacgggg cgcaatggtg cgcgtggcgc
    20761 acgcccgcgg gcggcacggt caaccagatt gaccgggtca tcgagcaaat ccggacgcag
    20821 cccgacagcc ggcggctcat cgtcagcgcc tggaatgccg gcgagctgga gcagatggcg
    20881 ctggcgccct gccacgcgct gtttcagttt tacgtgcagg acggggagct ggactgccag
    20941 ctataccagc gcagcgcgga cctgttcctc ggcgtgccgt tcaacatcgc atcctacgcg
    21001 ctcctgacga tgatggtcgc ccaggtctgc ggcctgaagc ccggcacgtt catccatacg
    21061 tttggcgacc tgcacctgta ttcgaatcac ctggagcagg cgcggcagca actgcggcgc
    21121 gagccgcgcc cgctgccgcg gatgcggctc aatcccgccg tgcgggacat tcacgcgttc
    21181 cagtacgccg atttcgagct ggccgattac acgccgcatc ccggcatccc ggcgccgatt
    21241 gcggtttagc cccgccggaa acgccatgcc cttcaaagcc attgccgcca tgtcgcttaa
    21301 ccgggtgatc ggcgccggca accgcctgcc ctggcatctg cccgaggatt tccggtggtt
    21361 caagcggatg accaccgggc aggtcattgt catggggcgc aagacgttcg agtccatcgg
    21421 ccggccgctg ccgcatcgca ccaccgtcgt gctgacgcgc gcgggggcgc cgattccggg
    21481 ggtccgggtc gtggcggatt tggggcagtt ggacgcggcg gatccggcgt ttgccgggcg
    21541 cgagattttc atctgcggcg gcgcgcagat ttaccagcaa gccctgccgc gctgttcgga
    21601 tctgtatctc acgctggtgc tgcgcgaggt tgagggcgac acgtttttcc cgccgtttga
    21661 ggatcggttc gcgctggccg gggaggtgct ggcccatccg gagttccgga ttttgcatta
    21721 ccaggcccga gtttagcctt tagccgcccg ccgtccgccg ttagccttcg cgaacgatgt
    21781 cccagatgct gaaatcctcc ggcgcgatgg ccgcggcgac gctgaccagc cgggtgctgg
    21841 gcatggtgcg cgagatggtc tatgcggggt tcatgggcaa cacctgggtg gccagcgcgt
    21901 tcaccctggc ctttcaggtg cccaacctgt tccggcggct gctgggcgag ggggcgctga
    21961 cggcggcgtt tattccgctc ttcaagcaaa aggagctgca ggagggggaa ccggagatgt
    22021 ggcgggcggc caacgcggtg atttccgggc tggtcgccgc ggcggcggcg gtggcggggc
    22081 tggtgatgct ggggattacg ctggcgctgg cgggggggtc gctggcggtg gagacgcagc
    22141 tcatgctgcg gctgctgcgg ctgatgtttc cctacatgct gctggtatgc ctggcggcgg
    22201 tgtttatcgg catggccaat gcgcgcgggc attttttcgt gccggcgctg ggggcggtgg
    22261 tgctcaatgt gatcatgatc ggcagcgtgc tgctgctggc gccgcggctg gggcggcgcc
    22321 tggaggagca gatattcggg ctggcgatcg gggtgttgct ggccgggctg gcccaggctt
    22381 ttttccagtg gccgagcctg gcgcgcgagg ggtatcgcta ccggtgggtt tcgccctggc
    22441 gcgacccgac cgtccgcgag gtggcgcgca aaatgttgcc gggctcgatt ggggtggctg
    22501 ccttccaaat caacgtgctg atcacgcagt gtttttcgtt ctggttcgag ccgaccattg
    22561 tggccacgtt caactacgcg gtgcggctga tggagctgcc gcaggggttg tttggcattt
    22621 cgctggccac ctatctgctg ccgacgctgg ccgggctggc ggcggagaag aagcacccgg
    22681 agttccgcca gaccctgggg caggggctgg gctatctgtc gtttgccaac ctgctggccg
    22741 ccgcgctggc actggcgctg gcggcgccga ttgtgcgcct gattttcgag catggcaagt
    22801 tcggccccga cgccaccctg cgggtggcgc tggcgctggc ctgtttagcg ccggggctgc
    22861 tgatgttttc gatgaacaac atcctggccc gcgctttcta cgcgttgagc gacatccaga
    22921 ccccgatgaa gatcagcctg gtgtgcctgg ggctgaacct cgggtttgcc ttctggctgg
    22981 tgcagtcgta ccgcgaggcc ggcctggcgg tggccaacac cctgagcgcc acgctgaacc
    23041 tggggctgct gctctatgcc ctgcgccgca agctcgggcg gctggagctg gccgggctaa
    23101 accgcgcgct gggggtgctg gtgcccaatg ccatcctcgc ggggggcgtg gcggcggcgc
    23161 tgtcctggct gtgggagcgg catctgggcc acgccacgct ggggttgaag ctgggcgcgg
    23221 tgtttgcgcc cggcggcgtg gcggggctgg gctactggct gctggccctg ggattcaagg
    23281 ttcccgccgc ccgggagatg acggagctgg cgtggcagcg gtggcggcgg cggccgcgcg
    23341 gctgagcggc gggctgctca ggcttcctcg accaccttcc agcccgcggc ttccgccagc
    23401 gcgtacacgg gctctttgag gtcgccgtag aaggtgacgc ggtgccagct ccactggtcc
    23461 cacatccgga acagcttttc cagatcgccg agcggctcgg cgcacagctt ggtgcggcag
    23521 gcgcggtcat ccgggtcatt ggccaccgcc agcgcgcggt ggagcaggat ttccttgcgc
    23581 gccgcgtcgc attcgaggga ggtcgtcagg tagccggtgg gcaggagcga gcggatggag
    23641 gcgccctggc ggtcttcgga gtgggtgagg atctcgtaag ggttggcctt gcccgccggg
    23701 ccgaacaccc gggtgggcgc cacacaatgg gcgtaaatga tccgccgccg ggaggtgtca
    23761 atcaccgggt cggagatgta gcccgggcgg ccttgcgtga gcgtggtccc aatgaccatg
    23821 gtggccgcgg agcgcacgtc acattcacag ccgccgacca gcccctcgtt gttcagttga
    23881 tggaagccca ggcagggata ggcgtggatg tggtttccgt aaaagccgcc caggcagttg
    23941 atggtgatgc cgcgcgcgcc gtgccgctgc aacagcgcct tttccgccag gtacatggcc
    24001 gcggagtttt cgatttcggc gcgcgtcacg ccttcgatcc gcttcgcccc ccgcatccag
    24061 cgctcggcca cggcgcgggc ttcgtccggg ttggcctgtt tccaggcttc gttcagctcg
    24121 gcaaagggaa cccgcaccac ttgcacgccc atcacctcgc cttcggggcc ggagctttgg
    24181 tcgcgcacgg ccaggatttt ggcggcctgc aactggcgca aacattcccg cggcgcgagg
    24241 cacggcaccg ggtcgcaccg cccggcggac cgcccggcgc gcggggttcc ccgccgccgc
    24301 gcgcgctcgg tggcggcgac gaaatccgcc gccgtcccgc cggggccggc cgcgcggaag
    24361 catttcaccg cgtccacgag gtcgcgcggg cgcgacgagg cgacgaagcc gacgttgggc
    24421 gtccggctgc gcaggagggc cgcggtatag acgaggaagc cgccgctgcc gccgaactgg
    24481 aagtcggcgt agaggacggg tttgccggag gtggcgatcg tctgcacgac gcggttccag
    24541 caattcatct ggcacacgag gtagccgtcc acgccgccgc ggtcggcgtc ctgttggagc
    24601 agcgcccggg cctgctcctc gccggtggcg gtcgagcgga tgaactcgaa ccccgggcag
    24661 tgccgggtca gcaggttcag gatgcgctcc atgtggggtt tgaaatcgaa gccctggttg
    24721 ggccagtccg ggcagggctg cttttccgcg tgcagcgcaa agatgacgcg gacgcgggtt
    24781 ttggcggcgg gcggggcggc gtcgagccac gccggggcgg ccagcgcgcc cagcgcgccg
    24841 gcggcggcgg cgccggtccg ggccagaaaa gtccgccggt tcagcgggca accggcgcag
    24901 gtgggaaagg taggagtcgt tttcataaac attcgagcgg gcggggaggg gttggggaga
    24961 tgcggggcgc ggggcgcgcc cgccgggatt ccggccgggg cggccggcgc gaacgcgttg
    25021 gccgggcgcg gcgtccggga gctggacagc gcgtttccat cacacccggg agcctatcgc
    25081 gggcgggttt ttcgtcaagc gccgaccggg ccgggggcgg cgccttttcg attgcgcatt
    25141 gcgcccgggc cgcggaggat gatgccccgt gaagaccttg catgcttatt tgatgcgcca
    25201 gatgctggcg tcgctggcgc tgaccgtgat ggtcttcaca ttcgtcctgc tgctgggcaa
    25261 cgcgctgcgg cagattctgc cgctgctggt gaaccgccag atgaccttcg gggtggtggc
    25321 cgaggcggcg gcgctgctga ttccctttgc ctgggttttt gcgctgccga tgggcctgct
    25381 gacggcgacg ctgctggtct ttggccggtt cagcgccgac caggagctga cggccgcccg
    25441 ggccagcggc atcagcctgc tgtcgttgat cagcccggtg ctgctgctga gcctggcgct
    25501 gtgcgggctg agcgcgctgt tcaacatgga aatcggcccg cgctgccgcg tcgcgtatga
    25561 taacatcctg ttcaacctcc gcctggagct ttccggcgtg caactgcccg aggggcggtt
    25621 catcaaggat ttcccgggct acacgtttta cgtggggaag aaccggcgcg gggatttgcg
    25681 ggacgtgatg gcctgggtgc tcgagcacga gaccaacgtg gtgacctatg tccgcgccgc
    25741 gcgcgggcgg ctggagacgg acgcggcccg caagagcgtc accctgcatc tttacgatgg
    25801 caaggtggtg gacttccgcg atgaccagat gatccccgcc gcgtttgcgg agctgccggt
    25861 gcggttggat cccaagccgc ggcggaccac gtaccagccc aagattgaca acatgaccgc
    25921 cacgcagttg tgggaggaat tgcgcgaatg ggaggcgcgg ctgaacgcgc cggagccggg
    25981 gcggcgttat tcggcggagg aattgcgcgc gcggcggcgc gagctggcgc ggcgcatggg
    26041 cgaccccacc acccccatcc gcttccagat tcatcagcag gtggccttct cgttcgcctg
    26101 cttcggcttc accctggtcg gcatcccgct gggcatccgg atgcaccggc gcgaaaccaa
    26161 tgtcggcttt ggcgtggccc tcattctggt gggcatctac tacgggttca tggtgctggg
    26221 ggagaccttt gacaagcagg cggaatatgc gccgcacctg attgtgtggc tgccgaactt
    26281 catcttccag gccgtcggcg ccgtgctcct gtggcgcgcg aaccgcggga tttgagccgc
    26341 cgccgccgcg cccggcgggc gccggtcagc gttgcgcctc cggggggcgc aaatggacga
    26401 gggtcgctcc ccagccgccg aattggggat aatcgaggct gaaggacacg acgtcggggt
    26461 gccgggccag gagcgcgtgc atcgtgcgtt tgagctggcc gaggcccttg ccgtggatga
    26521 tgcgcacctg gcggatgccg cgctcccggc aggccgcgag gtaatccggc accaggtctt
    26581 tgacctcgcg cgggcgaaag gtgtgcaagt ccagcacgcc gtcaatcgga agcggaatcg
    26641 gctcgttcat cacgctgcaa acggcgcgtc gcgcccggcg cgccgcgctg cggcggcggc
    26701 cgggaggcgg cggtcagact ttgggttccc agccgcggcg ataggtgcgg ctggccaggg
    26761 cggcggcggt tttgtcgccg acaatctttt gggtgcgggg gtcgaagttg agggtatggc
    26821 cggtgcgcca ggcgatgttg gccaggtggc acagggcggt gcttttctgg ccgtcctcga
    26881 tgtccgactt caggggtttg ccctcgcgga tggcgtcgag gaaattggcg aagtggatgg
    26941 cgtcaaagtc gaacaggccc gcgcgctggc tcttgatttc ccgcaccacc ttgttgctga
    27001 ggtcgtagat gacggtgttg ctgccggcca tggccaggca gccgttttcg ccgtagaaat
    27061 tgacgccgac ggtggcgccc tcgaagccgc gcggggcgca gctttgacat tcccaggcga
    27121 tgcccttgtc gccaaaatca aacgtggcgc tcatggtgtc cggggtttcc caatcgtcct
    27181 ggtggaaata ccgcgcgccg ccgcaggtga tgcgcgtggg caggtccacc tgcaggccca
    27241 ggcgggcgag gtcgagcatg tgcacgccgt tgttgccgct ttcgccggtg ccccagttcc
    27301 agaaccaatg ccagttgtag tgcaggatgt tgtcccggta gggctgctcg ggggccgggc
    27361 cctgccacag cgagtaatcg agccagtcgg ggacggggac cggccggccg cggccaatcg
    27421 aggggcggtg gttcatatac cagccgcggg cgaaaaagag tttgcccagc tcgccggcat
    27481 gcagggccgc gatggactcg atgacccacg gccagctccg gcgctggttg cccatctgca
    27541 cgacgcgccg gtatttgcgc gcggcggcga ccatgagccg gccttcgcgc aggttgtggc
    27601 tgccgggttt ttcgacatag acatgcttgc cggcggcgca ggccaggagg gtggcgggcg
    27661 cgtgccagtg ctcgggcgtg gcgatggaaa tggcgtccag gtcgggccgg tccagaatgg
    27721 tgcggaagtc tttgacgccc aggggctttt tcgcctgctt ccgggccacc gcggcgacgc
    27781 ctttctcgac ggcgcggctg tccacgtcgc agacgtagcc cacctccaca tggggcaggg
    27841 cgaggaaccc ggcaatgtgc gccatgccgc ggccattgca gcccacgacg gccacgacca
    27901 ccttttcatt ggggcttccg gcgccgcgca acagggcggc gtggcccagg gcgcccaggc
    27961 ccgcggcgag ggtggaggtt tgctgcagaa aacgacggcg cgtaatggaa tcagtcatgc
    28021 gatcatacaa cgcgtttggg gcggcgctgg caagcaaact tcgccccgcc ggcgcgccgg
    28081 ggcggcgggc gcggcttaaa tgccgaggtt ggcgagcgtg ctgctgagcg cgttgacgat
    28141 ctgcacgagg cggggatgcg agcgctcgaa ctcccggacg gaggagccga ggccgtcgag
    28201 cgagagccgg agcagctccg ggttctgcgc cgcgcgcgtg gcttcatggg cggagacttc
    28261 ggcgaacccg gcgatgcttt gggcctgctc cccgtgcgtc cgggacagtt cggtgatttc
    28321 cgacttgagc gttcccagca actggagcag ctcctgccgg ggcccggcgt caatggttcc
    28381 ggcgctttga atccgttttt cgatctggct gagggtgtcc tcgatcatgg cggtgctttc
    28441 ggtttccctc ccaacctagc gcccggcgtc cggatggcaa gccacattcg cgcgcggcgg
    28501 gggcgaaagg cgcgcccgtt cgccgccccg gcgggagggc gcgcgtcagc cggccgggcg
    28561 gcccttgccc gggaggagcg gctggaccag gtcccagatg gcggcctgga tgtccgcgac
    28621 cttcccggtg gcatccagca ggcgcacccg cccgggttcg gccgcggcga gggcctggta
    28681 gccctgggcc acccggtcga agaacgcccg gtcggcctcc tcgaaccggt cccgcttgag
    28741 ggacaacggc agcgtgggct ggcggacgcg gcggcgcgcc tcgctgagtt cctgcggcac
    28801 cacaaagacc agggtcaggt ccgggcgggt gccgcccacg gccacttcaa tcaggctttg
    28861 gaccattcgc aagtccagtt gccggccgta gccctggtag gcgatggtgg aatcgtaata
    28921 gcgatcgcac aggacgatct gtccggcggc cagggcgggg cggatgacct cgcggaccag
    28981 ttgggcgcgg ctggcgttca tgaggagcag ctcggcttcg ggggtcatgg cgtggttggc
    29041 cgggctgtgt ttgagcgtgt ggcggatttc ttcgccgatc gccgtgccgc cgggctcgcg
    29101 cagggtgcgg acggggtggc cctgggcgcg cagccgctcc gccagcaggg aaatctgcgt
    29161 cgtcttcccg ctgccctcgg tcccctcgaa ggtgatgagg cggcccggca tgttacaggc
    29221 gtttggcccg cgcgccccgg ggggtgtcct cgatcgtcca gccccgggcc ttgagctcct
    29281 cgcgcagggc atcggcgcgt ttgaagtcct tcgcctgccg cgcggcctgc cgggcttcga
    29341 ccagttgggt gatttccgcc ggcacgtcca cttccggcgg cgcgccgacg cccagcacgg
    29401 aatccacccg gttccaggcg gccagcgccg ccgccgccgc cggggcgtcc agcgcgttct
    29461 ccgccagctt gcggttggtg tcgcggaccc attcaaagac ggctccccag gcgcccgaga
    29521 tgttcaggtc gtcgtccagg gcttgcgtga acgcctccac cagcggcgct tcgcccgccg
    29581 ccggggcggc gccggccgcg gcgcgcaatt tcagcaggca ttcatcaatg cgcgccaggg
    29641 ccgcccgggc ggcctgcaat ccttcgaggg tgaaattgaa cgtctcgcga tagtgggccg
    29701 tgagcagcag gtgccggatt tcccgcccgg tgaacccccg ggccagcagg tcgcgcaggg
    29761 tgaaaaagtt gccgagcgac ttgctcatct tccggccttc gaccagcagg tgcgccccgt
    29821 gcagccagta tttgacaaag ggccgcccgg gcggctggag cgccgcgccc tcgctctggg
    29881 cgatttcatc ctcgtgatgc ggaaacatca ggtcttcgcc gccgaggtgc aggtcgaagc
    29941 tcggcccgag cagcttcatg ctcatggcgc tgcactcgat gtgccagccg gggcggccct
    30001 cgccccacgg gctgggccag aacacctggc cgtcctccgg cgtccgcgct ttccagagcg
    30061 cgaagtcggc caccgcctcc ttctcgtatt catcgctgcg cacccgctcg cccacgcgca
    30121 tggcctcgaa gttgagcttc accaaccggc catagcgggc gccgccgccg cgatacttct
    30181 cgatgctgaa atacaccgag ccctcggggg tggcataggc caggccgcgc gcctgcagcc
    30241 gctcgatcag ggctatgatg tccgcgatgt gctccgtcgc gcgcggcgtc tggtgcggcc
    30301 gccggcaatt cagcgcctcg aggtcgtcga aaaaggcttt ctcataccgg gcggtgaact
    30361 cccggagggg ctgcccggtt tcgcgcacgc gctggatgat cttgtcgtcc acatccgtga
    30421 tattcatcac ctgccggacc gcatagccct tgaaatccag gtagcgccgc accaaatcgc
    30481 cgaagacaaa cgtccgccag ttgccgatgt gggcgaaatc ataaaccgtc ggcccgcacg
    30541 cgtagaggcc gaccgtccgg ccggccggat ccaggggcac gaactcctgc accgagcggg
    30601 acagggtgtt aaacagcttt aaacccatag cgaatgcggg gaagcttagg ggccgggcgg
    30661 ggaaaggaaa ggcaaaactc ccggggcctc atcccgcggg ccgctccgcc gcggcgcgcc
    30721 cgctgtgcgg cggcccggac gccggcccgg gccgcccgaa cccggcgcgc gcctccccgg
    30781 gccgggggcg tcagttctgg ctgggccggg ggcgctgcaa gccgaccgag tgggcaatct
    30841 cctcaaactc ctcgcggctg acctcctcca gccgcttgcg ccgggccgcg agtttctcgt
    30901 tgatcttgat cgccttctcc gtcatggcct cgtgggtctc ttccgaaccg ccggccagga
    30961 tgaagttggg gccggtggtg agccgcttgt ggccgtcgga atcgaggccc aggccgagca
    31021 tgacggcctg gccgcgcggc ttctttttct tcgcattcgc catgcgcgga cgatacggga
    31081 ggcgcggcgg cgcgtcaacg cgcttcccgc gctgtcaggc cccgggcgcc gtcagcgcct
    31141 ggccgttatc ccgcgggccg agctcgagca ggaagggcac cgcgcggcgc gcccacgccg
    31201 ccggggacgg gtagccggcg gccgcgccgc caaagcagct ttgcagcatc tcggtgttga
    31261 tgatgcccgg gttcagggcg accgccgcca gcccgcgcgg cagttcccgc gccagcgccc
    31321 gcgtcagccc ttccaccgcc cacttgctgg cgcaataggg cgccacctcc gcatccgcgg
    31381 aacggcccca cccggaactg aagttcacga tcaccccgcg ccggcgctga atcatcgcgg
    31441 gcgcaaagtg gcggattaca tggacggtgc cgttgaggtt cacctccatc acccgcgcga
    31501 actccggcgc cggcacgtcc cacaggcggg cgttccggtt gatgaccccg gcgttgttga
    31561 gcacaagatc gggcggccca tgcgcgccca gcaggcggtc ggcccaggcc cgcacctgct
    31621 cgtccgcggc gatgtccacc gccgcgaagt catggggggg gccggcctgg cggcggagcc
    31681 gctcgaggtc cccgggcgac cggccacagc cccaaaccgt gtgccccagc cggatgaatt
    31741 catccgtcat ggcccggccc aggccccgcg ttgctcccgt aatcaatacg attctccggc
    31801 tcattgcttc gtccaaaaga ttgcccgccg ctccgcccgc ggctcgcgcc gcggggcgat
    31861 tcgtttcccg ttcacccgcc aagcgtaggc cgccccaaac ggattgccca gtacgaatcg
    31921 cggaagacgc ggcccaaaaa aagggaaagc atccgggttt attgccggga tgcggaccag
    31981 ttagcccgtt ttgaacgcag cgcggcgcgg gccacgaggc ggaccaaaac ctgccaaccg
    32041 gcaccggcaa tttgccgtgt gtttaggctt gacgaggggc cttggtttgg ctaaaaatac
    32101 tacatcgcgg gataataagt ttgaggtggc catcatgaag gcgctgaaaa cattcagccc
    32161 gccagccggt gcggcgatcg cactggcggg gagggccgct tcgtcccgcg cacaagaaag
    32221 gagattctat gaataagaac ctgagcagca aatccccggg aatcagcctt gggctgtgcg
    32281 cgctggcgct ggcggccggg ttgaccgcgc agggagcggc accggtcatc acgaacctca
    32341 cggtggtggg gggcgtggtg cagtttggcg tgcagagtga attggggctc accaaccgga
    32401 tccaatgctg cacgaatctg ggccagccgg agtgggaagt gttgacgaat ttggtggtca
    32461 gcgagagccc gtattggttc atggacgccg cggcggcggc ggtcgcgcag cggttttacc
    32521 gcgtggaggc gctggggagc agcgtctcgc cgcccccggg catggtgctg attcccgccg
    32581 gcagtttcac gatgggcgac tgtatggacc cgggcgaggg gtgggagaac gagttgccgc
    32641 tgcacacggt atatgtgagc gcgttttaca tggatcggta tgaggtgacc aaggcgttgt
    32701 gggacgaggt gaaggggtgg aatggcggca acggttacgt gtatgagaat gccggttccg
    32761 gaaaggcggc caatcacccg gtgcagatgg tcaactggcg ggactgtgtg aagtggtgta
    32821 atgcgcggag tcagaaggag gggttggtgc cgtgttactg caacgaggcg ggattgacga
    32881 cgatctataa ggccgggacg aacgcgccct acgcgaaatg ggctgcaaat gggtatcgtt
    32941 tgccgacgga ggcggagtgg gagaaggcgg cgcggggcgg ggtcagcggg catcggtttc
    33001 cgtgggctga cacggacaac atctcccaca gccgggcgaa ctattgggcg gttgtcaact
    33061 atccttacga cttgagttac ccggcaggtg atcatccgac cttcgctact ggcggccatc
    33121 cctatacgag tccggtgggg tattttgagg cgaacggcta tgggttatat gacatggcgg
    33181 ggaatgtggg tgagtggtgt tgggattggt attcggatga gtattatagc tcctcgcctg
    33241 ggactgaccc tcgcggcccc gcctcgggct gggaccgtgt gggtcgtgtg ggtcgcggcg
    33301 gcggttggtg ctgcgacgcg tacgtttgcc ggacggccca ccgcggctac ggcaacccgc
    33361 cggacggccg gagcaacctc atggggttcc gcgctgtccg tgccgcgggt cagtgagctc
    33421 aggcggccgg gcggttcggc ccggagccgg cggcaaatcg ggttgacaaa ttgcgcccgt
    33481 agctggacat ttgttc
//
LOCUS       DAOPQG010000031        32537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1789_length_32537_cov_16.488486, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000031 DAOPQG010000000
VERSION     DAOPQG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 32537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32537
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1789_length_32537_cov_16.488486"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..1459)
                     /locus_tag="PKI07_05205"
     CDS             complement(401..1459)
                     /locus_tag="PKI07_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50483.1"
                     /translation="MADLKFACPHCGQEIECDELWSGHQIQCPTCRKELTVPLKPEVS
                     PLATFASAKPGQAKLSIGSSRAQRSASERTVAPQAVALEQQLAQAKAGQKGAALKWVK
                     IGVGVLVVAVGGLVGYPYLRDWMVQRDAAAKSTSASTTQPPAATAEPAAPAETPVEKE
                     LPLLPPAWTLDVGQARIPESNANGVIAGSNFVVETARLDSMPGNIYLLRLVEGPLASP
                     ARAFMVYLRLNAGESILDRSWTISPDMKGKHVPQVVKLWKPNPRYQAQQKTFASGYVL
                     KLEFGKTANGVIPGKIFLAVPPETEQSVVAGIFKANTSLAGAIDAPAEKPAEAAESAA
                     EPLPTGATSFEQRYGGKR"
     gene            1670..2569
                     /locus_tag="PKI07_05210"
     CDS             1670..2569
                     /locus_tag="PKI07_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOC50484.1"
                     /translation="MNTTFAGLTVSHLLAGIIIAATLGLPGAAFAAPKKVLVVTVTTG
                     FRHSSIPTAEKVLAELGKASATFKVEYARVEPNEAQFKGPDGKPDEKKVNAAIEKVLA
                     EKMSRTALQQYDAVIFANTTGDLPLPDKQAFLDWVKSGKGFVGMHSATDTFHNFAPYL
                     EMIGAQFRTHGAQVKVDAINEDAACPICRHLPKNWKVFDEIYLFKNFDRTKVHGLLTL
                     DRHPNEKTPGDYPIAWCKEYGKGRVFYTSLGHREDVWDPDWPDRKNSREVAEAYQQHI
                     LGGIKWALGLEKMNARPPKVAVE"
     gene            2600..3973
                     /locus_tag="PKI07_05215"
     CDS             2600..3973
                     /locus_tag="PKI07_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50485.1"
                     /translation="MNQNKSESLGYNRRDFLKGGSAVTLMTMLGGVELFAQASAETSD
                     ETAKPAGKIKVAVIGLGAWGREIVNTLVRSSQADVAAICDTYSPFLKRTANAAPGATQ
                     TGDYKTILENKDIKAVIIATPTHLHKEIALAALKAGKHVYCEAPLAGSIEDAREIALA
                     AKAARQLVFQSGLQMRSDPLRHFLLPFIRSGALGKSVQARAQWHKKTSWRSASPNPEQ
                     EKALNWRLRQATSLGLIGEIGSHSIDQAGWFLNALPTAVTGFGSVAFWKEDRDVPDTI
                     QAVLEFPGGVRMFYDATLANSFDGEYEMLYGSDAAVMMRDGKAWMFKEVDSPLLGWEV
                     YADKMTFYKETGIAIVAGKSKAMPANEQPQPAQEATSTDTPLSVALGNFLRNAQDLAT
                     AAEDYEASYGSDDVEGLMEHLSAVPRRAAAGYLEGYQAVVTAVKANESILGGKRIELK
                     PEWYELA"
     gene            4037..4711
                     /locus_tag="PKI07_05220"
     CDS             4037..4711
                     /locus_tag="PKI07_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOC50486.1"
                     /translation="MKNHTLIAGTISFLLLTSAVTQAASQATTYNARSGSKMRIEGTS
                     NIHDWQVESPFIGGMMEVGPGFPTEPGQAATPGKMEVKANVFIQARSLKSVEKDGKPY
                     SDKMDEIMWEHLKEPQYKRIVYQLNELTLKEAPKTKDAPYVFDAKGELAVAGVTNKIA
                     MEVNILPLADNRLEITGKHTLKMTDFKIDPPAPKIALGLIKTGDEVKLIFKWIVGQRK
                     MPTSGN"
     gene            complement(4930..6744)
                     /locus_tag="PKI07_05225"
     CDS             complement(4930..6744)
                     /locus_tag="PKI07_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOC50487.1"
                     /translation="MPLAALAATAPLRFAWLSDTHVGSDTGEQDLNAAVNDINSLTGL
                     SFVVISGDVTEYGSREQFKRTKQSLDKLKLPYHILPGNHDAKWSESGATDFARFWPAD
                     RFVFEQGGFRFIGLHQGPLMKMGDGHWAPQDVRWLKETLAQLPDPNQPIIFITHYPID
                     NGIANWYVVLDLLKRYNTQVVLCGHGHGNQRYLFEGVPGVMGRSSLRANAAAGGFNLV
                     EIKDGVMTFSERITGRQTSPPWHSVKLQKHNYVTNSGNYPRPDFSVNSRYPGVKPRWI
                     YQSGYTIASSPAVWKDLAIVGDASGKVYALSLKTGRERWQFLTGNAVYATPDVSEGVV
                     IIASTDGTIYALKAASGKEIWRHVTDRPIVASPRIAGGRVYIGSSEGVFRALDLASGR
                     TVWQYDGLGGFVETKPLIHGGKVIFGAWDQHLYALEAATGKLIWKWKGDKPGVLYSPA
                     ACWPVSADSKVFIVAPDRKMTALNLQTGAEVWRTGDYVVRESIGLSQDQNRFYVRAMN
                     DFFYAFSPSASQPEKIWELNAGFGYDINSAMLVEKNGVLFYGTKNDLLFALDARTGAI
                     QWQHKLGTGVMNTVVPISATQVFATDFDGQIALIEAKP"
     gene            complement(6938..8479)
                     /gene="rimO"
                     /locus_tag="PKI07_05230"
     CDS             complement(6938..8479)
                     /gene="rimO"
                     /locus_tag="PKI07_05230"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOC50488.1"
                     /translation="MKEPTATRPARVGLVSLGCAKNLVDTEVMVGMLLRAGLEVTNEA
                     AQADVVIVNTCAFIDSAKEESVNAILQSVEWRNWKQRGQGVVVAGCLPQRFRSQLPVL
                     LPEVDAFVGLDQVPQIVDVVRQVLARRPPSQSCTSRGGTIEAPPLVRISPRSRYIPDY
                     ATPRFRLTPHHFAYVKIAEGCNHPCSFCVIPRIRGAYRSRPQADIVAEAKTLVASGVR
                     ELNLISQDSTCYGMDLRPDRGNFSRPERFGAARKSLSRPVSTISTLLRELNAVPGDFW
                     IRLLYTHPAHWTDELIQTIAECPKVARYLDIPLQHIHPNMLERMRRETSREHILNLIH
                     RIRAGIPGVTLRTAFIVGFPGETEACFEALLDFIRETRFERLGVFTYSREEGTRAGKM
                     GGQVPEKIKQKRRNLALAAQRKIAAQVAESFVGRTIKVLIEGSANLAELRRTHTNPRE
                     YASARRKLRLAGKPAGRFLMARSEADAPGIDGRVYIQGQLPVGAFAWVKIIDHMDYDL
                     IAEPD"
     gene            complement(8476..9186)
                     /locus_tag="PKI07_05235"
     CDS             complement(8476..9186)
                     /locus_tag="PKI07_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50489.1"
                     /translation="MKCLSPYTEPVEPQMRSEWAKSVARQLCRDSLAWAKTILAVAGA
                     ELAKPKGAGGDGASRKEQLFAEVLAYSLCLLEMRLTKDLAAGNKDLARRVRHECAMLI
                     AQAARRKQNRHRRLPVPDPANSRYAQIYPAPGSAGRRPPRLTREMFEQFCRCTGMESN
                     VLVGKGENLATLVFYIAVHGVISADGLPSREAMLKLLEAARACSAHLQGVAAKWAGSR
                     RTDGQLSFPASRKWIGLG"
     gene            complement(9183..10484)
                     /locus_tag="PKI07_05240"
     CDS             complement(9183..10484)
                     /locus_tag="PKI07_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOC50490.1"
                     /translation="MAKRVPGTDHDPGSETGARANPVLAGKLLRSEGQPANLPGAWPQ
                     FRGPQRDAISAEAVSLERSWAPGQPRELWAIDVGEGYAGPVILNGRVYLMDYDRDQQQ
                     DALRCLSLADGREIWRYSYPVRVKRNHGMSRTVPVVTDQLVVAIGPKCHVVALNSITG
                     ELLWGLDLTRQFGATVPPWYAGQCPLLDQGAVILAPGGDDALLLAVEAQTGKVLWRTP
                     NPHGWKMTHSSVMPMEFDGQRMYVYCASKGVVGVSAQDGSLLWETPDWKISIATVPSP
                     LPLDGGRLFLTGGYNAGSLMLQLANDGGRLAPRTLFKLAPEVFGATQHTPIRYHDHLY
                     GVRADGKFVCLTLDGKVAWASGSGQQFGLGSFILANGLILAMNDSGLLRLIEALPQKY
                     TLLAQAQVLKGRESWAPLALAGGRLIVRDLTRMVCLEVGCP"
     gene            complement(10753..11400)
                     /locus_tag="PKI07_05245"
     CDS             complement(10753..11400)
                     /locus_tag="PKI07_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOC50491.1"
                     /translation="MIAPPAATETKRLKVLWQYEVLDTVPEEVFDDLTELAATICEAP
                     MALISLVDENRQWFKSKIGLTISETSRGHSFCAYAITQPGLFIVPDATLDPRFASSPM
                     VVSEPKIRFYAGAPLITPDGHALGTLCVLDKVARQLGPNQREALRILAHHVVSQLELR
                     RRSRELNNARQKNRHLQHQLSQLRAELAAAHDQLAKRAPRVCPQSPGRRKKRGQT"
     gene            complement(11479..12222)
                     /gene="allE"
                     /locus_tag="PKI07_05250"
     CDS             complement(11479..12222)
                     /gene="allE"
                     /locus_tag="PKI07_05250"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HOC50492.1"
                     /translation="MTDLFGFSRNAVHERYAVFASGSVASSYLPGWEKAVCQVLISPA
                     LGARFSQILIALTKEGHCAGNTGANQYFVYLLEGAATILIEARKHRLEPGSYAYLPAG
                     QDVQIASAAAAARLLIFQKRYEPLPQLAQPVGFVGHEREVKGQPIRGADGMRWQSLLP
                     GQPAFDMAVNLVTCQPGATLPAVEVPGMERGLLMVRGQAIYRLADDWHPVQAGDVVWM
                     ASYCPQWFAAVGQAPASFICYQNANRDPM"
     gene            12692..13627
                     /locus_tag="PKI07_05255"
     CDS             12692..13627
                     /locus_tag="PKI07_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50493.1"
                     /translation="MKILSSPRTGSLGAETYYQSPFGVCARRRTVPRDRPSERKAAAR
                     SYFGISSREWGLKLSEAQRERWNAAALHVPSRPWMGQYSHLSGQQFCVQINSTLRGLG
                     LAPVEEPPAPVVFGPNPVTGLEIVNDAEDGVRLRLNVGAVHEDIMVFGQPPCSAGRMK
                     PRRAYYLGLLGPSQNGQSDITDLYTARFGRPAPGQKVFIAVVQTRNGWKGQSRVHNAI
                     VPPPPMLKSQQAAETAKVKKSKPSAAPASTAAHKTVSSPSFRAMYKGSTSEARQEHME
                     NKHESPLSLLCTPVVHGLMGALGWLGRVGRVWARA"
     gene            complement(14176..15291)
                     /locus_tag="PKI07_05260"
     CDS             complement(14176..15291)
                     /locus_tag="PKI07_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOC50494.1"
                     /translation="MTEPEPLQQIDRTFVRYRRRKLSYYSGCDYFRLASHPRVMAALQ
                     TGVKKYGLNVAASRLTTGNHALYRELETRLVEFFDAESALLVPSGYATNLITAQALAG
                     NFSHALIDERAHTSLADAARLLDCPVLRFKSRDAAALAQAARRCGPAAKLVVLTDGMF
                     SGDGSAAPLAEYLALLPRDAMLVVDDAHGAGVLGRTGRGTLEHAGVSRDRIIQTITLS
                     KALGSYGGAILCSQTFRHRILSQSRMFVGSTPLPLPLAQAALTSLRILKSTRTLRPRL
                     LRNAARVKTALRQAGLALPDAPGPIIPLLPRHPDEAARLSRSLFAAAIFPPFLKYPGG
                     PPGGCFRFVISSEHNDAQLDNLIQVLRRHLTLPPASA"
     gene            complement(15323..16531)
                     /locus_tag="PKI07_05265"
     CDS             complement(15323..16531)
                     /locus_tag="PKI07_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOC50495.1"
                     /translation="MDLESLVALAANNGASDLHLEAGLPAAIRIRGELRTTGDPLPAN
                     LLAEWARGVIGEEEWPRFLEQRSYDCSRNYRGCRCRLNILMTSRGIGFAIRLLSSFIA
                     TIEKLNLHPDFKKLVTGHHGLILVSGATGSGKSSTLAALIEQINMTETRHILTIESPI
                     EFTFRPRRAYIRQREVGRDTPSFEQALIDSLREDPDVLMVGEMRDPETMRLTLAAAET
                     GHLVMATVHSSTCAEALQRVVSAFPAEIQSSVAAQLADCMLAVISQRLCFRPEFNIRV
                     PECEILVPTHAVKNFIRNRDFFKISSSLETGAEHGMWTVQRYRAWLDSRKNWYIPGKS
                     PDPGENEPGSAPPPPLRPPAFPLAPRPSKSPARPAPPAHGSSPKAPARIEIEPGESEF
                     GKILKRPGEK"
     gene            complement(16623..17399)
                     /gene="panB"
                     /locus_tag="PKI07_05270"
     CDS             complement(16623..17399)
                     /gene="panB"
                     /locus_tag="PKI07_05270"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOC50496.1"
                     /translation="MTGIKITAADIRAMKSRGEKIAALTAYDFPTTRLLDQAGIPLIL
                     VGDSLGMVVLGYPDTTSVTMAEMEHHVRAAARSRPRALLAADLPFRSYETAADALANA
                     QRLVAAGAEAVKAEGGREILDQVRAIVAAGIPFLGHLGMLPQHVREENGYHLKGKSEA
                     ERLKLLADAQALAEAGAFAIVLELVSPRVAQAITRQVPIPTIGIGAGPDCDGQILVTP
                     DLLGLQPWYQLKHVKPKLNAAAQIQAAIAEWKQDLQRPAG"
     gene            complement(17461..18507)
                     /locus_tag="PKI07_05275"
     CDS             complement(17461..18507)
                     /locus_tag="PKI07_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC50497.1"
                     /translation="MKPSSLSASRARQILVRARQKQILVVGDAMLDHFVQGSVGRISP
                     EAPVPVLDFESESFMPGGAANVARNLTALQVRTELIGAVGRDAAGAQLKHLLGEHRIG
                     CRGLIADATRTTSVKTRVVAHKQQVVRIDRESHAELKPALSSRLLRTLETMIPRSAAV
                     IVGDYGKGVVTQPLLDELKQICRAHGVWLSMDPKPVHRLDLSGLSLITPNRKEAFEMA
                     GLADETRQSRPLKDPSLLRVADYLLRTLRPAVLLITLGELGMLLCQHGKKPFHIPTVA
                     RDVFDVSGAGDTVIATFTLAIAAGASPVEAAIISNHAAGIVVGKTGTATTAPAELLAS
                     FPRLPGKARPASFG"
     gene            18599..19405
                     /gene="hisA"
                     /locus_tag="PKI07_05280"
     CDS             18599..19405
                     /gene="hisA"
                     /locus_tag="PKI07_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007276806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HOC50498.1"
                     /translation="MAFASQALGWGVFRPCIDIHEGKVKQLVGGTLGEEPGRLRTNFV
                     SDRPAAWYAELYKRDGLTGGHVIMLGPGNEAQAREALQAYPGGLQIGGGINLDNAPGW
                     LEAGASHLIVTSWVFRQGQVDWERLAQLRAAIGRARLVLDLSCRRRGKDYFVVTDRWQ
                     KFTEVAIGPANLERLAGFCAEFLIHAVDVEGLCRGIDQELVSRLGQWSPLPTTYAGGA
                     RLLADLETVTRLGQGRVDLTIGSALDIFGGRGVRYKDAVEFNRRCQASKT"
     gene            19468..22560
                     /gene="secA"
                     /locus_tag="PKI07_05285"
     CDS             19468..22560
                     /gene="secA"
                     /locus_tag="PKI07_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOC50499.1"
                     /translation="MIKYILTKFIGSKNDREIRRLRPLVARINALETELQKVSDDVLR
                     QKTAAWKEELSRIQDDKELARRLDEILPEAFAVVKNACRRLCGTEIIVREHPLKWEMI
                     PFDVQLIGGYALHTSRIAEMATGEGKTLVATLPVYLNALSGRGVHIVTVNDYLAARDS
                     EWMGAVYKFLGLTVGCILHDQPPRVRREQYNCDITYGTNAEFGFDYLRDNGMASRKEE
                     QVQRGHYYAIVDEVDSILIDEARTPLIISGPAVVTHDEQYANFRPQVEALFHVQERLC
                     NRFLAEAQELLRKLHPADGSNPEHPEELERQIGLLLFRVKCGQPKSGGLLKLLEEPEN
                     LRLMNQAELQLHADQKKIELYKEKEELFFAIDEKSHETDLTEKGRNYLSPKDPDAFML
                     PDLPTALHTIDTGPEEDARKRLEAKTRLQQEFETKAQRIHAISQLLKAYCLYQKDVQY
                     VVQGNKVIIVDENTGRLMTGRRWSDGLHQAVEAKEQVEIERETQTLATITIQNYFRLY
                     HKLAGMTGTAETEASEFFDIYKLGVLVIPTNRPVARKDANDSVYKTKREKYNAVLKEI
                     KEIHGQGRPILVGTISVEVSEHLSRMLKREGIIHSVLNAKFHQQEAEIVARAGQRGSV
                     TIATNMAGRGTDIKLGPGVADSGGLHVMGTERHEARRIDRQLRGRCARQGDPGSSHFY
                     ISLEDDLMRLFGSDKIVKYMEKMGLEEGQELEHPLLNRSIGQAQKRVEQHNFQIRKRT
                     LEYDDVMNKQREVIYGFRNEIIHSADVRDRLMDIMEEVVLQKVEQFTTDETDLHDWKV
                     RSLADWVNLNFPIGMPEKEIIKAAEAGKEEPVKGSIFENLSPAQFSVCTFISDNVRKA
                     YEIKISFEDPEALKEVERYTILNAIDKLWQEHLYEMDSLRYSIGLRAHGQRDPLIEYK
                     AEAYKIFDELMVNIKTEICHNIFRSASSLMAFENFLHNVPQQATHQSTSAFGGTTTGT
                     SSGAGLAPRASQQGSDIVSEAAAAVERAKPVRAGPKVGRNDPCPCGSGKKYKQCCGRN
                     "
     gene            23574..24305
                     /locus_tag="PKI07_05290"
     CDS             23574..24305
                     /locus_tag="PKI07_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC50500.1"
                     /translation="METPQGPGGCFNTTHWSVVLRAGRSDTPRARAALDQLCRHYWQP
                     LYAYVRRAGYSREEAEDLTQEFFARLLAQNSVARADPMRGRFRSFLLSSLKHFLMNEW
                     DKARAQKRGGGVPVLSLAFDAAETSCASAVAPGDTPDRAYDRQWVLALLEVVLGRLRE
                     EHQAAGREGTFLGLKDTLAGGRSEIPYRELGARLGMSEGAVKVAAHRLRARYRELLRE
                     EIANTVARPEEVEKELRELFAALAY"
     gene            24400..27258
                     /locus_tag="PKI07_05295"
     CDS             24400..27258
                     /locus_tag="PKI07_05295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC50501.1"
                     /translation="MLSSSEILLIFLALLVTAGIAAAVIAFVVWLTRRGAAGMPDKAP
                     IQQPPPSTSAPARRQGRKFAFVEERGGKLRVRWWVCCVSMGVGVLATALVLELFLRIG
                     GWRNEKVWIPAIVVGAGSICAWLIYEFSQAREARATGWPASAPASPIPGGTVALAACP
                     RCGAALPADAPQGLCPRCVLGVGLATHTEATGESGPHGTKVVQPPPEPAEIAKHFPQL
                     EILECLGRGGMGVVYKARQPKLNRLVALKILAPERGADPQFTERFLREAQALARLNHP
                     NIVTVHDFGEAEGLYYLLMEYVDGMTLRQLLHGQKIAPEEALSIVPKICEALQFAHEL
                     GVVHRDIKPENVLVDWQGRVKIADFGIAKIVAADQPRQAITQDQVIGTPHYMAPEQVE
                     KPQLVDHRADIYSLGVVFYEMLTGELPLGKFQPPSKKVQVDVRLDEVVLHALEKEPER
                     RYQHASEVKTAVETIAATPGAPAACAPAGPPVVIKRWRDAWIWNWEYIQLFLIVPIVA
                     AGLALPFLLPRLGLRALWMFAAALGGIAFAITYAIVGRQVRRARAEVSRTERDVAECL
                     MFRRPFQSPGVAVMHEARLELIPIVGSPIIVPLADIVAVKEVRWFNGQRLFFKRGFVM
                     DLANGQRVGVAVAEVFARRWRAKLSRGTLPEIPAGSGRDQSLLASAAAKKWMMAARWT
                     ARVLGTLLFAFYGLFLFGEGVPPIGSQPEGVQLSFVGVGLMLLGFVVGWQREGAAALL
                     IASGWTVFRIAEGGFGLGAFEAPLLVAALYGLYWWVTQVRKTHLALAAVAALVMALTW
                     GQLICPTSVFVRGMIIDAETTRPIADAELTLEGSPEKRGEEGMHPNTRSRKDGRFTLY
                     VGWYAEQKKMTIAAPGYETLTTNLGPRALGQRKVSRTYLLRRVGNPTSGQSEGARREF
                     MRLVAAKDAVPVEGMPTTRGAARWGRGWRGCRIGRTRC"
     gene            28002..28430
                     /locus_tag="PKI07_05300"
     CDS             28002..28430
                     /locus_tag="PKI07_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC50502.1"
                     /translation="MNTNPKTPSPPCTAVLVDDDLTSRWLLRGVLKRLGFTLLAEASN
                     GEEAVAVVRRTKPDIVLLDVCMPFQTGPGALPAIVAAHPPAQIVMLTSMADEATVRYC
                     IEQGAAEYLRKDTPVEEISRVLGGLSEKIAAQREREVRCA"
     gene            28423..28605
                     /locus_tag="PKI07_05305"
     CDS             28423..28605
                     /locus_tag="PKI07_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50503.1"
                     /translation="MREPKPPRFDLDTLLAEIPQDEAVCRTAPRLLSQDDIRRLIEES
                     RRRKERAAPRVSPHEK"
     gene            28595..30292
                     /locus_tag="PKI07_05310"
     CDS             28595..30292
                     /locus_tag="PKI07_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOC50504.1"
                     /translation="MKSEAFLKLVVDRGLVSAEQARTLSARHGKVALAILEELIDRHE
                     TAKVELCQMYGDALGVAYLDLTRTIIQPEVVRLLPRDFAVKHQMIAVYRFADSVTVAT
                     AEPLNPTVLDDAARLLSLPVNAVFSLPGEIKDAIEVEYTTVTVLQESQAQMGLKHVFV
                     EETEATPQRLAGVAADQSIVQLSRQLFICALRERASDIHIEPSQHEVRVRLRIDGVLQ
                     ERFHLDRRLLAPLASRLKIMAGCDIAERRRPQDGRIAFELATRSLDVRFSCVPGMDGE
                     KIALRLLGTVRGRPIPLLEELDFSHACLGTVKQIAASPSGVFFVTGPTGSGKTTTLYS
                     VLKLLNSPGVHIVTVEDPVEYRLPGTTQVQVNPDVDLTFLTALRSFLRQDPNIILVGE
                     VRDLETAKMVSQAALTGHLVMSTLHTNSAIQAITRLVEIGVEPFLVASALAGVSAQRL
                     VRRICPNCREEIPMPRSAIERYFGPLGDRTVNMWHGRGCDACHRTGYSGRLAIHEVFM
                     LSEEIRRLIAEKATPPELQSLAEQYGYRPMVYDGIKKVLRGLTTLAEVERVTAIPEE"
     gene            30395..31390
                     /locus_tag="PKI07_05315"
     CDS             30395..31390
                     /locus_tag="PKI07_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOC50505.1"
                     /translation="MLFLACALTLVGCATRPPSPPESQGPPGARTPMGLIAATADLAA
                     AGATRLHPNYIWATLKDRKQDAALNTAFERALTEGTPRAQQALLRALELKFIHDPQTS
                     GLDILGPELSHHFRTFAWQAEDFPGGPPGPNEQFADVMVDVLDLVRPERRANSTRDAV
                     VLREEATEAVWEYMTRQWVPVPGQENWKLNRHARESFIRMREQARAEGVDLVILSAHR
                     LRKTAEANAARARNPNAVASFSAHSLGLAVDFDMSHRNDIKFSGLSTRPMSELVRMRE
                     SPVHKWLLLRGEEFGWYPYQNEPWHWEYNPPGFREIFWADFPGGAPERGIIIEEP"
     gene            complement(31573..32094)
                     /locus_tag="PKI07_05320"
     CDS             complement(31573..32094)
                     /locus_tag="PKI07_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC50506.1"
                     /translation="MNISTRRWTRADLATVQQLLLETWLEAYGSFIPQTDLEGYLHTQ
                     YSELRLAALFADPDVTGLVAEVDGRVAGYAKLFHARAELKFYVHQLYILPAWQGHGLG
                     HRLMTCAEERARELGADRIWLGVMVKNTQAVEWYKRLGFTITETAPFVMGATTVDHYI
                     GYLPLPLPRSRPR"
BASE COUNT         6437 a         9323 c        10659 g         6118 t
ORIGIN      
        1 gtcgcgccgc ttgctctcaa tgcaccctct gctatcggct taagcacagt ggatatacgg
       61 gaatcgctct taacgttaat gctggcgttt ccggcctctc ggcgaaacgc ctcgcgccga
      121 gcctcgggaa gactttccca cttgttcgag cgatccgagt ttgaggcgtc catgttcgag
      181 gaagaagccc tattcgtttc gttggtttgg ctagatccgc gaaggcatac caaagcaact
      241 gctgccacag tgcataagag cgcccaaaac ggttttgctc tcataaggaa ggacatttcc
      301 ttctcaggat gtctctcgcc atgcacactc aaatcaaagc gagacaatcc ccgcttatca
      361 agcaagatct cccggacggg atttcctggg ggcggaagaa ctagcgtttg ccgccgtaac
      421 gctgctcgaa agaggttgca cccgtcggca gaggttcggc ggcagattcg gcagcttccg
      481 ccggtttttc ggccggggcg tcgatggcac cagccaagct ggtattcgcc ttaaagatgc
      541 cagccacgac gctctgctcg gtctccggcg gcacggcgag gaatattttc cccgggatca
      601 ctccgttagc agtcttgccg aattcgagtt tcagaacgta cccggaagca aatgtcttct
      661 gttgcgcctg gtagcgggga ttgggtttcc agagtttaac cacctgcggc acgtgtttgc
      721 ctttcatgtc gggtgagatg gtccaggagc gatccagaat gctttcacca gcgttcagcc
      781 gtaaataaac cataaacgcc cgggcagggg atgccaacgg accctcgacc aaccggagca
      841 gatagatgtt gccgggcatg ctatcaaggc gagcggtttc aacgacgaag ttcgagcccg
      901 caatcacacc attggcgtta ctctccggaa tcctggcctg gcccacatca agggtccagg
      961 caggtggcag caggggcagt tccttttcca ccggcgtctc tgctggcgcg gcgggttcgg
     1021 cggtggcggc gggcggttgg gtggtgctgg cgctggttga tttggctgcc gcgtcgcgtt
     1081 ggaccatcca atcccggaga tatggatatc cgaccaaacc acccacagct accacaagca
     1141 cgcccacgcc gatcttcacc catttcaggg cggctccctt ttgtccggcc ttcgcttgag
     1201 ccaactgttg ttccagcgcg acggcttgcg gggccactgt tcgttcggaa gcggagcgct
     1261 gcgcccgaga tgagccgatg gagagttttg cctgacccgg cttggcgctt gcgaacgtag
     1321 caaggggcga aacttcgggc ttgaggggca cggtcaactc tttccggcag gtaggacatt
     1381 gaatctgatg cccgctccaa agttcatcac attcaatttc ctgtccgcaa tggggacacg
     1441 caaatttcag gtcagccata gcttttactt gttgccagcg ccccacattc accgccaacg
     1501 gctggttgtg cggcacgggc ctgatcggga ggcatataca acatggcaga gcctcgccgt
     1561 caaggaaatg gcgagagaga attcttgact gcggcaggga aaatgaattt cattatgtat
     1621 gattgagtca tagaactcag atgctcaggc gtggtcgttt gctaaataca tgaacacaac
     1681 tttcgcaggc ttaacggtga gccatttgct ggctgggata ataattgccg ctacgctcgg
     1741 cctgccgggg gcggcatttg ctgcccccaa aaaggtgctt gtagttacgg ttaccaccgg
     1801 atttcgccac agttccattc ccacagcaga aaaagtgctg gcggaactgg gcaaagcaag
     1861 cgccacattc aaagtcgaat atgcgcgggt agagccgaat gaagctcaat ttaaaggccc
     1921 tgatggcaaa ccggatgaaa agaaagtcaa tgccgccatc gagaaggttc tggccgagaa
     1981 gatgagccgt acggcactgc aacaatacga cgccgtgatc tttgccaaca cgaccgggga
     2041 tttgccgttg ccggacaagc aggcatttct ggattgggtg aagtcgggca agggctttgt
     2101 cgggatgcat tcggccactg atacttttca caactttgcc ccgtatctcg agatgatcgg
     2161 ggcccagttc cggacacacg gggcgcaggt gaaagtggat gcgatcaacg aggatgccgc
     2221 ctgcccgatt tgccggcatc tgcctaaaaa ctggaaggtg ttcgatgaga tttatctgtt
     2281 taagaacttt gaccgcacca aggtgcatgg tctgcttacg ctcgacaggc atcccaatga
     2341 aaagaccccg ggggattatc cgatcgcctg gtgcaaggag tacggcaagg ggcgtgtttt
     2401 ctatacctcg ctgggacatc gcgaggatgt gtgggatccg gactggcctg accgcaagaa
     2461 ttctcgcgaa gtggcggaag cctatcaaca gcacattctg gggggaatta aatgggcctt
     2521 gggattggaa aaaatgaatg ccaggccccc aaaagtggcg gtggaatagt ttttcatcta
     2581 gattcttttc aagtccttta tgaaccaaaa taaaagtgag agtttgggat ataaccggcg
     2641 cgattttctt aaaggtggat cggctgtcac gctgatgacc atgctgggcg gggtagaact
     2701 gtttgctcaa gccagtgccg aaaccagcga tgaaacggcg aagccggcgg ggaaaataaa
     2761 ggttgcggta attgggctgg gcgcgtgggg ccgcgaaatt gttaacaccc tggtccgttc
     2821 gtcgcaagca gatgtcgcgg ccatttgtga tacctatagc ccatttttga aacgcaccgc
     2881 caacgccgcg ccaggcgcca cccaaacggg agattacaaa accattcttg aaaataagga
     2941 cattaaagcg gtcattattg ccacgccaac acacttgcat aaggagattg cactggccgc
     3001 gctcaaggcg ggaaagcatg tctactgcga agcgccgtta gctggctcaa tcgaagacgc
     3061 acgggaaatc gcgctggcgg ccaaagccgc caggcaactg gttttccaga gcggcttgca
     3121 gatgcgctcg gatccgttgc gtcattttct gttgccattt attcgttcag gagcactggg
     3181 taagagcgtt caggcacggg cgcaatggca caaaaagaca agctggcgct cggcttcgcc
     3241 caatccggag caggaaaaag cgcttaactg gcggttgcgc caggccacgt cgctcggcct
     3301 gattggtgaa ataggcagcc attcgattga tcaggccggc tggttcctga acgcactgcc
     3361 caccgcggtc accggctttg ggtcggttgc cttctggaag gaagaccggg acgtgccgga
     3421 tacgattcag gcggtgttgg agttcccggg gggagtgcga atgttttatg acgccacgct
     3481 agccaattcg tttgacgggg agtacgagat gctctacggc agcgatgccg cagtcatgat
     3541 gcgcgatggc aaggcgtgga tgttcaagga agtagattcg ccgctgctgg gctgggaagt
     3601 ttatgccgac aagatgacct tctataagga gaccggcatc gctattgtag ccggcaagag
     3661 caaggctatg ccggcaaacg aacaacccca acctgcgcag gaagcaacca gcacggacac
     3721 tcccctttca gttgccttgg gcaacttcct gcgcaacgcc caggatctcg cgacggcggc
     3781 ggaagactac gaggccagct acgggtcgga cgatgtggag ggcctgatgg agcacctgtc
     3841 agccgtgccc cgacgagccg cggcaggtta cctggaaggc tatcaagccg tcgttacggc
     3901 cgtgaaagcc aacgagtcca tcctgggcgg caaacgaatc gaacttaagc cggaatggta
     3961 tgaattggct tgagcgagca aacgtcaaac catgcagaca agcaagcaga cgcgataaac
     4021 aaataagacc ataaatatga agaaccacac actgattgca ggaaccatct cttttttatt
     4081 gctgacttcg gcggttacgc aggcagccag ccaggcgacc acctataacg cgcggtcggg
     4141 tagcaagatg cgcattgagg gcacgtccaa catccatgat tggcaggtgg aaagcccgtt
     4201 tatcggaggg atgatggaag tcggcccggg ttttcccacg gaacccggcc aggccgcgac
     4261 tcccggcaaa atggaagtca aagcgaacgt gtttatccag gcacgctcgc tcaagagcgt
     4321 tgaaaaggac ggcaagccct atagcgataa aatggacgag atcatgtggg aacacctcaa
     4381 agagccgcag tacaaacgca ttgtttacca gcttaatgag ctgacgctca aggaagcgcc
     4441 caaaaccaag gatgctcctt atgtgttcga tgccaagggc gaactggcag tggccggagt
     4501 aaccaacaag atcgcgatgg aggtgaatat tttgccgctg gcggacaacc ggttggaaat
     4561 caccggcaaa cacacgctga agatgaccga tttcaaaatt gatcctcccg ctcctaaaat
     4621 tgcgttgggc ctcatcaaaa ccggcgatga agtgaagctg attttcaagt ggatcgtggg
     4681 ccaaaggaag atgcccacct ctggaaattg atagtgggga gagccagcag gagttggttg
     4741 tcgttcttaa cgcgacgggc ctaacgagag tgttcgtcgg aaacggccgg cggcgtggct
     4801 tgtgcggagg aaatgctatt tattctccgg caatctgcag cccaatgcgg aagcgaggct
     4861 tccccgttgt atttgtggga ggcggactat ttcacaaggc caacacggga aagaatgcgc
     4921 atccgtgatt tacggtttgg cttcaatcaa ggcgatttga ccatcaaagt cagtggcgaa
     4981 aacttgcgtt gcgctgatcg gcacgaccgt attcatcacc ccggtgccga gtttatgctg
     5041 ccactggatc gcaccggttc gggcgtccag cgcgaagagc aaatcattct tggtgccgta
     5101 gaataaaacg ccgttctttt cgaccagcat cgccgagttg atgtcgtagc caaacccggc
     5161 gttaagttcc caaatcttct ccggctggga agcactggga gagaaggcgt agaagaaatc
     5221 gttcatcgcc cggacataaa aacgattttg gtcttgagac aggccgatag attcgcgcac
     5281 gacatagtct ccggtgcgcc agacctcggc gccggtttgg aggttcagcg cggtcatttt
     5341 gcggtcggga gcgacgatga aaaccttgct gtcggcactt acaggccagc aggcggcagg
     5401 ggaatagagc accccgggtt tatcgccctt ccacttccag atgagttttc ccgttgctgc
     5461 ttccaaggca taaagatgct gatcccaggc gccgaaaatc actttgccgc catgaatcaa
     5521 cggtttcgtt tcgacgaaac cgcccaagcc atcgtattgc caaaccgtgc gcccggaggc
     5581 cagatcgagc gcgcggaaaa cgccttcgct ggaaccgata tacaccctgc cgcctgctat
     5641 gcggggtgag gcaacgatgg gacggtccgt gacatgccgc cagatttcct ttccgctcgc
     5701 ggccttgagc gcatagatag ttccatcggt ggacgcaatg atcacaacgc cttcgctcac
     5761 atccggcgtg gcgtaaacgg cgtttccggt aagaaactgc cagcgttccc ggccggtttt
     5821 gagggatagc gcgtacacct tgccagatgc atctccgacg atagcgaggt ccttccaaac
     5881 cgccggagaa ctggcgatgg tgtagccact ctgataaatc caacgcggct taacgccggg
     5941 gtagcgcgag ttcaccgaaa agtcgggacg cgggtaattc ccggaattag tgacatagtt
     6001 atgcttttgc agtttcaccg aatgccaggg gggcgatgtt tgccgaccag tgattcgttc
     6061 agagaaggtc ataacgccgt ccttaatctc gaccaggttg aagccccccg cggcagcatt
     6121 cgcgcgcagg cttgatcggc ccatgacgcc gggtaccccc tcgaagaggt agcgttgatt
     6181 gccatgtccg tggccgcaca ggacgacctg cgtattatag cgcttaagga gatcaagcac
     6241 gacgtaccag ttggcaatgc cgttatcaat cgggtaatgc gtgatgaaga tgattggctg
     6301 gttcggatcc ggcagttgtg cgagggtttc cttcagccag cgcacgtcct ggggcgccca
     6361 gtggccatcg cccattttca tcagcggccc ttggtgcaga ccgatgaagc ggaaaccgcc
     6421 ctgttcgaaa acgaagcggt ccgccggcca gaaccgcgca aaatccgtcg ctccggactc
     6481 agaccacttg gcgtcgtgat ttcccggcag aatgtggtac ggcagcttca acttatccag
     6541 agactgcttt gtccgcttaa actgctcgcg ggaaccgtac tccgtcacat cgccggagat
     6601 caccacgaag cttaacccgg tcagcgaatt gatatcgttt acggcggcgt tcaagtcctg
     6661 ttcgccagtg tcgctaccga cgtgagtatc gctgagccag gcgaatcgca gcggggcagt
     6721 ggcagccaga gccgccagcg gcataagata aagcagcacg cagggcacag cacggcgcag
     6781 aaggcagacc attcgcgtca ataagcagta tttttgcata ggtctatatt cccctgcgtt
     6841 gcggtttgct gcaagttaag atcccggtca ggcaggctcg ctcttcccgt ggatgtggca
     6901 actgtccttg cggttggagg tttggccaag actgtcctta atcgggttcg gctattaggt
     6961 cgtaatccat gtggtcaata atctttaccc atgcgaacgc gcctacgggt aactggccct
     7021 gaatgtagac ccggccatca attccgggag cgtcggcttc gctgcgggcc atcaggaatc
     7081 ggcccgcggg cttgccggcc agacggagtt tgcggcgagc cgaggcatat tcccgcggat
     7141 tggtgtgagt tcgccgcaat tccgccaggt tggccgaacc ttcaatcagc accttaatcg
     7201 tgcgtcccac aaatgattcg gcgacttgtg ccgcgatttt gcgctgcgcc gccaaggcaa
     7261 ggttgcgccg tttttgttta atcttttcag gaacctgccc gcccattttt ccggcgcgtg
     7321 tcccttcttc gcgcgaatag gtgaatacgc ccaggcgctc aaacctcgtt tcacgaataa
     7381 agtccagcag ggcttcaaaa caggcttccg tttcgccggg aaacccaaca ataaaggccg
     7441 tgcgcaaagt gacgccggga atgccggcgc ggatgcggtg gatcagattg agaatatgct
     7501 cgcgcgaggt ctcgcggcgc atccgctcga gcatgttcgg gtgaatgtgc tgcagcggaa
     7561 tgtccagata gcgcgcgact ttggggcact ccgctatcgt ttgaatcagt tcatccgtcc
     7621 aatgcgcggg atgcgtgtag agcaggcgaa tccaaaagtc tcccggcacg gcatttaact
     7681 cacgcagcag cgtgctgatg gtggaaaccg ggcgcgaaag cgatttcctg gcggccccga
     7741 atctttccgg cctggagaag ttgccacggt cggggcgcaa atccatcccg tagcaggttg
     7801 agtcctgtga aataaggttc aattcgcgca ctccgctggc tacgagagtt ttggcttcgg
     7861 ccacgatatc ggcctgcgga cggctacggt acgcgccccg gatgcgcgga atgacgcaga
     7921 aactgcaagg atgattgcaa ccttccgcaa tcttcacata ggcgaagtga tgcggcgtaa
     7981 gccggaatcg cggggttgca taatcgggga tgtagcggga tcggggagag attctcacca
     8041 ggggaggagc ctcaatggtg ccgcctcggg acgtgcatga ttgagacggc gggcggcgcg
     8101 ccagcacctg gcggacgaca tccacgattt gcgggacctg gtcaaggccc acgaaagcat
     8161 ccacttccgg cagcagcacc ggcagttgac tgcgaaaccg ctgcggcagg cagccggcga
     8221 caaccacccc ctggccgcgc tgtttccagt tgcgccattc caccgattgc agaatcgcat
     8281 tcacgctctc ttccttggct gaatcaataa aggcgcaggt gttgacaatc accacgtccg
     8341 cttgcgcggc ttcgttggta acttccaggc cggcccgcaa cagcatgcct accatcacct
     8401 cggtatccac gaggttcttc gcgcagccga gcgaaaccaa acccacccgg gccggtcggg
     8461 ttgccgtcgg ttctttcatc caagaccaat ccacttgcgg ctggctggaa acgaaagttg
     8521 tccgtcagtc cgcctgcttc ccgcccactt ggccgccacg ccttgcaaat gggcgctgca
     8581 agcccgcgcc gcttccagca gctttagcat cgcctcgcgc gagggaagcc catcggcgga
     8641 aataacccca tgaacggcaa tatagaaaac cagggtggcc aggttttccc ctttgccaac
     8701 caggacattc gattccattc ccgtgcagcg gcagaactgc tcaaacattt cccgggttag
     8761 ccggggcggg cgcctgccag ccgagccggg cgccggataa atctgcgcgt aacgggaatt
     8821 ggcagggtcg ggaaccggga gccgccgatg gcggttttgt ttccgccgcg cggcctgcgc
     8881 gatcagcatg gcgcattcat gccgaacccg cctggccagg tctttattcc cggccgccaa
     8941 gtccttcgtc aaacgcattt ccagcaagca taaactgtaa gccagaactt cggcaaacaa
     9001 ctgttccttc cgcgatgccc cgtccccgcc agcgcctttt ggtttagcca gttccgctcc
     9061 ggctacggcc aggatggttt tggcccaggc cagtgagtcg cggcacaact ggcgcgcgac
     9121 ggatttcgcc cattcagagc gcatttgagg ctcgaccggc tccgtataag gggagagaca
     9181 tttcatgggc atcccacttc caggcagacc atgcgcgtca ggtcgcgcac gatgagccgg
     9241 cccccggcca atgccagtgg cgcccaggat tcccgcccct tcaatacctg cgcctgggcc
     9301 aagagcgtgt atttctgcgg aagagcttcg atgagccgca gaaggcccga atcattcatg
     9361 gccaggatca atccattggc gaggataaac gaaccaagcc cgaattgttg gccggagccg
     9421 ctggcccagg ccaccttgcc gtcgagcgtc aggcagacaa atttgccgtc ggcgcggacg
     9481 ccgtagagat gatcgtgata gcggatgggc gtatgctggg tggcgccaaa gacttccggc
     9541 gcaagtttga agagtgtccg cggcgcgagg cgtccgccat cgttggcaag ttgcagcatc
     9601 aggctgccgg cgttatagcc gccggtgaga aagagacgcc cgccgtccag gggcagaggc
     9661 gagggcaccg tggcaatgct gattttccag tctggcgtct cccacaggag cgagccgtcc
     9721 tgggcggaga cgcccaccac gcccttgctg gcgcaataaa catacattcg ctggccgtcg
     9781 aattccatcg gcatcacgga ggaatgggtc attttccagc cgtgcggatt gggggttcgc
     9841 caaaggacct tgccggtctg cgcctccacg gcgagcagca gggcgtcatc gccaccgggg
     9901 gcgaggataa ccgcgccctg atcaagcaac gggcactggc cggcatacca ggggggcacg
     9961 gtggcgccga attggcgcgt caaatccagt ccccacaaga gttcgccggt gattgagttc
    10021 agggcgacga cgtggcactt ggggccgatg gccacaacga gttgatccgt aacgacaggc
    10081 acggtgcggg acatgccatg gttgcgcttt acgcggaccg ggtagctata acgccaaatc
    10141 tcccgcccat ccgccagcga gaggcagcgc aacgcatcct gttgctgatc gcggtcatag
    10201 tccatgagat agacccggcc gttcagaatc acggggccgg cgtaaccttc gcccacgtca
    10261 attgcccata attcgcgcgg ttggcccgga gcccaactcc gttcgaggct gaccgcttcc
    10321 gcgctgatcg catcgcgctg agggccgcgg aactggggcc acgcgccggg aagattcgcc
    10381 ggctgcccct ccgagcgcag gagcttgccg gctaaaaccg ggttggcccg ggcccccgtc
    10441 tcgctgcccg gatcgtggtc agtccccggc acccgcttcg ccatgggcgc gcccgggccc
    10501 agatgaagcc agagcgcata tgcccccaat gccagcagcg ctagtgcggc cgggatcagc
    10561 aaggctatcc tgttcgcggc tggcagtttc atcaaacggc acctggcatc gaggggcggt
    10621 ccccggggag aacttcgccg gctcggcggc gcgcgcgcgc gcgcattccg cctttatcgg
    10681 agcggggaaa tcgttcgcca ctgcgaccgt gaattgagcc ggcaggcaaa ccggaattgg
    10741 ggacggcgct ggttatgtct gacctctctt ctttcttctg ccgggggatt gggggcagac
    10801 gcggggggca cgcttcgcga gctgatcatg ggcggcggcc agttccgctc gcagttgact
    10861 caactggtgc tgcagatgcc ggtttttctg gcgggcattg ttcaactcgc gggagcggcg
    10921 gcgcaactcg agttgcgaaa caacatggtg cgccagtatg cggagtgcct cgcgctggtt
    10981 cgggccaagc tggcgcgcga ctttatcaag gacgcagagc gttcccagcg catgcccatc
    11041 gggggtaatc aacggcgccc cggcatagaa gcgaattttg ggttcggaaa ccaccatcgg
    11101 gctgctggca aaacgagggt ccaatgtcgc gtcgggcacg ataaacaagc ccggctgggt
    11161 gatggcatac gcgcaaaacg aatgcccgcg cgaggtctcg ctgatggtga gcccgatctt
    11221 cgacttgaac cattgccgat tttcgtccac cagcgagatc aaggccatcg gagcttcaca
    11281 aatcgttgcg gccagctccg tcagatcgtc aaacacctcc tccggcacag tgtcgagcac
    11341 ttcgtattgc caaagaactt taagccgttt cgtctccgtt gctgcaggcg gggcaatcat
    11401 gttgtcagcg ggttgcgcca gccatagccg ggcttcacgg cgccgattga taagcgccca
    11461 gggccgccat gactggaatt acatcgggtc gcggttcgca ttctgatagc agataaagct
    11521 ggccggggcc tggccaaccg ccgcaaacca ctgcggacaa taggaggcca tccaaaccac
    11581 gtcgccggcc tgcaccggat gccaatcatc cgccaaacgg tagatggctt ggccgcggac
    11641 cattaacaag ccgcgctcca tgccgggcac ttcgaccgcc ggcagagtgg ctccgggctg
    11701 acaggtcacg agattcaccg ccatatcaaa cgccggctgc ccgggcagca gggactgcca
    11761 gcgcatgcca tcggcgcccc ggatgggctg gcctttgact tcgcgctcat gcccgacaaa
    11821 ccccaccggc tgcgccagtt gcggcagagg ctcataccgc ttttggaaaa tcaacaagcg
    11881 cgccgccgcc gccgcgctgg cgatctgaac gtcctggccg gcgggaaggt aggcatagct
    11941 gcccggttca aggcgatgct tgcgagcttc gatcaggatg gtcgccgccc cttcgagcag
    12001 gtaaacgaag tattgatttg cgcccgtgtt gcccgcgcaa tggccttcct tggtcagcgc
    12061 gatcaatatt tgggagaacc gcgcccccaa cgcgggcgag atgagcacct ggcacactgc
    12121 cttctcccaa cccggcagat agctggaggc aacactgccg gacgcaaaca cggcatagcg
    12181 ttcgtggaca gcgttgcgcg agaaaccaaa taaatctgtc ataattccga tactcactcc
    12241 ggccttacga aatgagtcta ggcgaggcca acccctgccc gcaaggagga gttgcatctt
    12301 cctgccacaa ccagccgtag ccgttgtatt ggcagcagaa cgggccggaa ccaaggccaa
    12361 aaggccggag agctgttctc gattgagaac cgcgcaatat agagccacgc tggagtgtcc
    12421 gagccgggct ttgcccggaa gattgtgaag gccacgtaac accccagacc ggaagaactg
    12481 cctcttgatg cacgcggcgc tccgcaccgg cccgccaccg ctaaccccaa aaaattgccc
    12541 ttgcgcgaag ccaccccact cgccagcctt ctcgcgtggg ctcggggtgg tggcggcggg
    12601 aacttccagc ccaaagaatg gcaagaagcc gagccgccct ccggggcgat gacagaaaga
    12661 cgataagaaa acaacaaaaa ggacaatcat tatgaaaata ctatcctctc cgcgcacggg
    12721 cagtctgggc gcggaaactt attaccaatc ccccttcggc gtgtgcgccc ggcggcgcac
    12781 cgttccccgg gaccgaccct cggagcgcaa ggcggccgca cggtcgtatt ttgggatctc
    12841 ctcgcgggag tggggtctca agctcagcga agcgcagcgc gagcgttgga acgccgcggc
    12901 gctgcacgtt cccagccggc cctggatggg gcagtattcg cacttaagtg ggcagcagtt
    12961 ctgcgttcag atcaacagca ccctgcgggg gctgggtctg gcgccggtgg aggaaccgcc
    13021 cgccccggtg gtcttcggcc ccaatcccgt caccgggctg gagatcgtca atgacgccga
    13081 agacggggtt cgactgcggc tgaatgtcgg ggccgtccat gaagacataa tggtctttgg
    13141 gcagccaccc tgcagcgcgg gccggatgaa accccgccgt gcctattacc tgggtctgtt
    13201 gggcccaagc cagaacgggc aaagcgacat cacggacctc tatacggccc ggtttgggcg
    13261 acccgccccg gggcagaagg tatttatcgc cgtggttcag acccggaacg gctggaaagg
    13321 ccaaagccgg gttcacaacg ccatcgttcc gccgccgccg atgttgaaat ctcagcaagc
    13381 ggctgaaacg gcaaaggtta agaaatcgaa gccctccgcc gccccggcct ccaccgccgc
    13441 ccacaagacc gtctcttctc cgtcctttcg agccatgtac aaggggagta catcggaagc
    13501 tcggcaggag cacatggaaa ataagcatga gtcccctttg agcctccttt gtacacccgt
    13561 tgtacacggc ctaatgggag cgttaggctg gctggggcgg gtggggcggg tttgggcgcg
    13621 ggcctgagcc ggaaaggagg aatggacgag tcgcggatga tgtaaaacga ggttccaagg
    13681 cgggagggag aggacgagag cgttcggcac agctggaacg ggcggatggg tgttgtcgcg
    13741 tgccttggtt tttatcgtga aggtgatgtt gaattgtcgg agcgttgcat tgccgaggct
    13801 agtttcattt tggggttgtc tgattgtctg cgacggcttt ggggtttgga tggaaggctc
    13861 aggtttggac ggatggcgtt ggggcggagt aatgttgtgg ggaggagatg gcgattgcca
    13921 agctgggcat ggggaaagag tctaacggct gaacaaatta aacaggagga gaaggaaaca
    13981 aggcagggca cttgtccgcg tttcgagagg ggcaagatgg aaagcctcac gaggaacctc
    14041 gcaagctcgg cgtgggcggc tgaactgcct ttacccgggg tggccggctt tgctgaagga
    14101 ggccctttta tattcgggtt ctcgccggag agaaatcgag gggccaaaag gcgcgcctgc
    14161 cagaagcccc gggcgttaag cggaagcggg aggcaaggtc aagtgacggc gcaacacctg
    14221 gatcaggttg tccaattgcg cgtcgttgtg ctcgctggag atgacaaaac gaaagcagcc
    14281 gccgggcggg cctccagggt acttgaggaa cggcgggaag atggcggcgg caaaaaggct
    14341 gcggctcagg cgggcggctt cgtccgggtg ccgggggagc aaggggataa tggggcccgg
    14401 ggcatcgggc agcgccaggc cggcctggcg caatgccgtt tttacccgcg ccgcgttcct
    14461 caacagccgt ggccgcaaag ttcgggtgga tttcaggatg cgcaggctgg ttagcgccgc
    14521 ctgcgccagc gggaggggca ggggggtgct gccgacgaac atccggcttt ggctcagaat
    14581 ccggtggcgg aaggtttggg agcagaggat tgcgccgcca taggagccga gggctttgct
    14641 cagcgtgatc gtctggatga tgcggtcgcg gctgacgccc gcgtgttcga gcgttcctcg
    14701 tccggtgcgc cccagcaccc ccgccccatg cgcatcatcc accaccagca tcgcgtcccg
    14761 ggggagcaac gccaggtatt cggccagggg cgcagcggag ccgtcgcccg agaacatccc
    14821 atctgtcagc acgaccagtt tagcggctgg accgcaacgg cgggcggcct gggccagcgc
    14881 cgcggcatcc cgggacttga agcggagcac ggggcaatcc agcagccgcg ccgcgtcggc
    14941 aagcgaagtg tgcgcccgct cgtcaatcag cgcgtgggag aaatttcccg ccagggcctg
    15001 cgcggtgatt aagttggtgg cgtagccgct gggaaccagc agcgcgcttt cggcgtcaaa
    15061 gaactccacc agccgggttt ccagctcgcg gtaaagcgcg tgatttcccg tggtcagacg
    15121 cgaggcggcc acgtttagcc cgtatttttt cacgccggtt tgcagcgccg ccatcacgcg
    15181 cgggtggctg gcgaggcgga aataatcgca gccagagtaa taggagagct tgcgccggcg
    15241 ataccgcacg aacgtgcggt caatttgctg cagcggttcg ggttcagtca tcggaacagt
    15301 ttataccggg cgccgcgggt tcttactttt cccccgggcg cttcaggatc tttccaaact
    15361 cgctttcgcc cggctcgatc tcgatgcgcg ccggcgcctt ggggctggac ccgtgggcgg
    15421 gcggagcggg ccgcgccggc gatttcgagg gccggggcgc gaggggaaaa gcgggcgggc
    15481 gcaatggcgg cggcggggcg cttccgggct cgttttcgcc ggggtcgggg ctttttcccg
    15541 ggatgtacca gtttttgcgg ctgtccagcc atgcccggta tcgctgcacg gtccacatgc
    15601 cgtgctcggc gcccgtttcc agcgacgagc tgattttgaa gaagtcgcga ttgcgaatga
    15661 agttttttac cgcgtgcgtg gggaccagaa tctcacactc gggcacgcgg atattgaact
    15721 cggggcggaa gcacagccgc tgcgaaatga ccgccaacat gcaatcggcc agttgcgccg
    15781 ccacgctgct ttgaatttcc gccgggaacg ccgagaccac ccgctggagc gcctccgcgc
    15841 aggtcgagga atggacggtg gccatgacga ggtggccggt ttcggcggcg gccagggtca
    15901 ggcgcatggt ttcggggtcg cgcatttcgc ccaccatcag cacgtcgggg tcttcgcgca
    15961 gcgaatcaat gagcgcctgc tcgaagctgg gagtgtcgcg cccgacctcg cgctggcgga
    16021 tataggcgcg ccggggccgg aaggtgaact cgatggggct ttcgatggtc aggatgtggc
    16081 gcgtctcggt catgttgatc tgttcgatca gggccgccag ggtcgaggat ttgccggagc
    16141 cggtcgcgcc gctgaccagg atcagcccgt ggtggccggt gaccagcttt ttgaaatcgg
    16201 ggtgcaggtt gagtttttca atggtggcga tgaaggacga gagcagccgg atggcaaagc
    16261 caatcccgcg cgaagtcatg aggatgttca gccggcagcg gcagccgcgg tagtttcgcg
    16321 agcaatcata ggagcgctgt tcgaggaacc ggggccattc ctcttcgccg atgacgccgc
    16381 gcgcccattc cgccagcagg ttcgccggca gggggtcgcc ggtcgtgcgc agttcaccgc
    16441 ggatccgtat cgccgccggc agccccgcct ccagatgcaa gtcgctggct ccgttgttgg
    16501 ccgccagcgc gactagtgat tccaggtcca tgctgcaatc ctattccaca gcgccgcccc
    16561 gggtaaagga ttccttggcg gccagggggt ggccgcgttt ttcgccgcgg tgcgcggccc
    16621 attcagcccg ccggccgctg caggtcttgc ttccattccg cgatcgcggc ctgtatttgc
    16681 gccgccgcgt tcagtttagg tttcacgtgc ttgagttggt accagggctg cagccccagc
    16741 agatcggggg tcacgaggat ttgtccgtcg caatccgggc cggcgccgat gccgatcgtg
    16801 gggatgggaa cttgccgggt gatggcctgg gccacgcgcg gggaaaccag ttcgagcacg
    16861 atggcgaaag ctcccgcctc cgccagggcc tgggcatcgg ccagcaattt caggcgttcg
    16921 gcctcgcttt tgcccttgag gtggtagccg ttttcctccc gcacatgctg cggcaacatt
    16981 cccaggtgcc ccaggaaagg gatgccggcg gccacgattg cccggacttg atccagaatc
    17041 tcgcggccgc cctcggcttt gacggcttcc gccccggcag cgaccaggcg ctgggcgttg
    17101 gccagcgcgt ccgccgcggt ttcgtagctt cggaagggca ggtcggcggc cagcaaggcg
    17161 cgcgggcggc tccgggcggc ggcgcggacg tggtgctcca tttcggccat ggtgacggag
    17221 gtggtgtccg ggtagcccag caccaccatt cccagcgagt cgcccaccag gatcagggga
    17281 atgccggcct ggtccaggag gcgggtcgtc ggaaagtcgt aggcggttag cgcggcgatc
    17341 ttttcgcctc gcgatttcat cgcacggatg tccgccgcgg tgattttgat tcccgtcatg
    17401 cttcaaccat cgaggcagaa ccggggcagg acaagtggaa aatcaaggcg ggcggaaagg
    17461 tcagccgaag gaagcaggcc tggcctttcc cggcagccgc gggaaactgg cgagcaattc
    17521 ggcgggggcg gttgtggccg tgcccgtttt cccgacgacg atgcccgcgg cgtggttgga
    17581 aatgatggcc gcttccaccg gggaagcgcc ggcggcgatt gccagggtga acgtggcgat
    17641 gaccgtatcg cccgcgccgg atacgtcgaa cacgtcgcgg gcgacggtgg ggatgtggaa
    17701 aggctttttg ccatgctggc agagcagcat cccgagttcg cccagggtga tcaggagcac
    17761 cgccgggcgg agagtgcgca gcaggtaatc ggccacgcgc agcaagctgg ggtctttgag
    17821 cggccggctt tgccgggttt cgtcggccag gccggccatt tcaaaggctt ccttgcggtt
    17881 gggggtaatc agcgaaaggc cggacaagtc cagccggtgc acgggtttgg ggtccatgct
    17941 cagccagacg ccgtgcgcgc ggcaaatctg cttgagttca tccagcagcg gctgggttac
    18001 cacgcctttg ccgtaatcac cgacgatgac cgccgccgag cggggaatca tcgtctcgag
    18061 ggtccgcagc agccgtgagc tgagcgccgg cttcagttcg gcgtgcgact cgcggtcaat
    18121 gcgcaccacc tgctgtttgt gggcgaccac gcgcgtcttg acgctggtgg tgcgcgtggc
    18181 gtcggcgatt agcccccggc agccgatgcg gtgttcgccg agcaggtgct taagctgcgc
    18241 gcccgccgca tcgcggccca cggcgccgat cagttccgtc cgcacctgga gggccgtcag
    18301 attgcgcgcg acgttggccg cgccgccggg catgaaactt tcgctctcga aatccagcac
    18361 cggcaccggg gcttccggcg aaatacgccc gacgctgccc tgcacgaaat ggtcgagcat
    18421 ggcatcgccc actaccaata tctgcttttg cctcgctcgg accagaatct gccgcgcgcg
    18481 gcttgccgag agactggatg gcttcatgtc cgcgccatta agacgcaatg ccgcggattt
    18541 gtcgagtggg atggcgcgga gcgtttcccg gtttcagggc ggggggttga tttaggcttt
    18601 ggccttcgcc agccaggcgc taggatgggg cgtgtttcgt ccgtgtatag atattcatga
    18661 gggcaaggtg aagcagcttg tcggcggcac gcttggcgaa gagccgggaa gattgcggac
    18721 gaactttgtc tcggatcggc ccgcggcctg gtatgccgag ctgtataagc gcgacgggct
    18781 cacgggtggg catgtgatta tgctcggccc cggcaatgaa gcccaggcgc gggaagcctt
    18841 gcaggcctac ccgggagggc tgcaaatcgg cggcggaatc aacctggata atgccccggg
    18901 ctggctggag gcgggggcgt cgcacctgat cgtcacctcc tgggtatttc gtcaggggca
    18961 ggtggactgg gagcgcctgg cgcaactgcg ggcggcgatt ggccgcgcgc ggctggtttt
    19021 ggatttgagc tgccggcggc gcggcaagga ttattttgtg gtgactgacc gttggcagaa
    19081 gtttaccgag gtggcaattg gcccggcgaa cctggagcgg ctggcgggtt tctgcgcgga
    19141 gtttcttatt catgccgttg acgtggaagg gttgtgccgc gggattgacc aggagctggt
    19201 ttcccggttg ggccagtggt cgccgcttcc caccacctac gccggcgggg cgcggttgct
    19261 tgccgacctg gagacggtca cgcggctggg gcaggggagg gttgatctga cgattggctc
    19321 ggccctggat atttttggcg gccgcggcgt acgctacaag gatgccgtgg agtttaaccg
    19381 ccggtgccag gcgtccaaga cctgaactgc tttctctttg acgggcgggg ggcgttcccc
    19441 tagcgtttag aggttctcac accgtacatg atcaagtaca tcttaacaaa gtttatcggt
    19501 tcgaagaacg accgcgagat tcggcgcctt cggcctttag tggcccggat caatgccctc
    19561 gaaaccgagc tccagaaggt ttctgacgac gttttgcgcc agaaaaccgc cgcctggaag
    19621 gaggaattat cccggattca ggatgacaaa gagctggcgc ggcggctgga cgagattctg
    19681 ccggaggcgt ttgccgtcgt gaagaatgcc tgccggcggc tttgtgggac cgagattatc
    19741 gtgcgggaac acccgctgaa gtgggagatg attccttttg acgtgcagct tattggcggt
    19801 tatgcgctgc atacgtcgcg gatagcggaa atggccaccg gagaagggaa gacgctcgtg
    19861 gccaccctgc cggtctatct caacgccttg agcggccgcg gggtccatat cgtgaccgtt
    19921 aatgattacc tcgcggcgcg cgacagcgag tggatgggcg cggtgtataa gtttctcggg
    19981 ttgaccgtgg ggtgcatttt gcatgatcag ccgccgcggg ttcggcgcga gcagtacaac
    20041 tgcgacatta cctacggaac caatgcggaa ttcgggtttg attatctgcg ggacaacggc
    20101 atggcctcgc gcaaggagga gcaggttcag cgggggcatt attacgctat cgtggacgaa
    20161 gtggactcga tcctgattga tgaagcgcgc acgccgctca ttatcagtgg tccggcggtg
    20221 gtgacgcatg acgagcaata tgcaaatttc cggccgcaag tggaggcgct tttccatgtg
    20281 caggagcgcc tctgcaaccg gtttctcgcg gaagcgcagg aactgctgcg caagttgcac
    20341 ccggccgacg gctccaatcc cgagcacccg gaggaactgg aaaggcaaat tggcctgttg
    20401 ttattccgcg tcaagtgtgg ccagcccaaa tcggggggcc tgttaaagtt gctggaggaa
    20461 cccgagaacc tccgcctgat gaaccaggct gaactgcagt tgcacgccga ccagaagaag
    20521 attgagctct acaaggagaa ggaagagttg ttcttcgcga tcgacgaaaa gagccacgag
    20581 acggacctca ccgaaaaggg gcgcaattac ctcagcccga aggacccgga tgcctttatg
    20641 ttgccggacc tgccgacggc gttgcatacg atcgacacgg ggccggagga agacgcgcgc
    20701 aagcggttgg aagcgaaaac caggctccag caggagtttg aaaccaaagc ccagcgcatt
    20761 catgccattt cccagttgct caaggcgtat tgtttgtacc aaaaggacgt gcagtatgtc
    20821 gtccagggca acaaggtcat catcgtggac gaaaacacgg gccgcctgat gacggggcgg
    20881 cggtggagcg acggcttgca ccaggcggtt gaggccaagg agcaggttga gatcgagcgt
    20941 gagacgcaga cgctggcgac gattaccatc cagaactatt tccgccttta ccacaagctg
    21001 gcgggcatga ccggcacggc ggaaaccgag gcctccgagt tctttgacat ctacaagctg
    21061 ggcgtgcttg tcatccccac caaccggccg gtggcgcgca aagacgcgaa cgattcggtt
    21121 tacaagacca agcgcgagaa gtataacgcc gtcctcaaag agattaagga aatccacggg
    21181 cagggccggc ctattttggt cggcaccatt tcggtggagg tcagcgagca cctgtcgcgc
    21241 atgttaaagc gggaggggat catccactcg gtgctcaacg ccaagtttca ccagcaggag
    21301 gcggaaatcg tcgcccgcgc cggtcagcgc ggctccgtga ccattgcgac gaatatggcc
    21361 gggcgcggca cggatatcaa gctggggccg ggcgtggcgg attccggggg cttgcacgtc
    21421 atgggaaccg agcggcacga agcgcgccgg attgaccggc agttgcgcgg ccgctgcgcc
    21481 cgccagggcg acccggggtc ctcgcatttt tacatctcgc tggaagatga tttgatgcgc
    21541 ttgttcggct ctgacaagat tgtcaaatac atggagaaga tggggcttga agagggccag
    21601 gaactggaac acccgctgct gaaccggtca atcggccagg cccagaagcg cgtcgagcag
    21661 cataacttcc agattcgcaa acgcacgctc gagtacgacg acgtgatgaa taagcagcgc
    21721 gaagtcattt acggcttccg caatgagatt atccattccg cggacgtccg cgaccgtctc
    21781 atggacatca tggaggaggt ggtgctccag aaggttgagc agttcactac ggacgagacc
    21841 gatttgcacg attggaaagt gcggtcactg gccgactggg tgaatctgaa ttttcccatc
    21901 gggatgcccg agaaggaaat tatcaaggcc gccgaggcgg gcaaagaaga gccggttaaa
    21961 ggctccattt tcgagaacct cagcccggcg cagttttctg tttgcacctt catctcggac
    22021 aatgtccgca aggcctacga gattaaaatc agctttgagg acccggaggc gctcaaggag
    22081 gtcgagcgct atacgatctt aaacgccatt gacaaactgt ggcaggaaca tctctacgag
    22141 atggacagtc tccgctacag cattggattg cgcgcgcatg gccagcggga tccgcttatc
    22201 gaatacaagg ccgaggcgta caaaatcttt gatgagttga tggtcaacat taagaccgag
    22261 atttgccata atatcttccg cagcgcctcc agcctgatgg cctttgaaaa ctttttgcat
    22321 aatgtacccc agcaggctac gcatcaatcc accagcgcct tcggcggcac cacgacgggg
    22381 accagttcgg gcgccggtct tgcacctcga gcatcccagc agggcagcga tatcgtcagt
    22441 gaggctgccg cggccgtcga gcgggccaag ccggttcgag cgggccccaa ggttggccgc
    22501 aatgatcctt gcccatgcgg cagcggaaag aaatacaaac agtgttgcgg caggaattga
    22561 ccggccgagg tggagggatt ttcaggcgca tattccggcg caaaaaaaac gagagaagac
    22621 cggagtcttc tctcgtttta gcttggactg attcggtagc gcttgtcagc tccaaatcgt
    22681 tcccgttctc aggcaaccca ctgtgccact ttcagcgggg ccttggatcg caagacgggc
    22741 tgtttgaatt agtcggcttc cagagcggcg attaatttgg gcaggattac ttcccgggaa
    22801 aagagagcgc ggacgtctgc aggcaattgg cggatggtcc gctctgtatc agcggcgagt
    22861 tgctgaatgt gttttaccat ccgttccacc tccctgtgct gggccgcgag atcccaaacg
    22921 agcccacaat atcaccccaa aacacgaaag ggcgaagggc cgagagcggt cgtttggtgc
    22981 agattggctt accgtgggaa cgccatgaca atgggccgaa actccgattt gctccgcgcc
    23041 aagcggaaaa ccaattaaga aagagcggcg ccgaaccttg ccgccataga atggagcaca
    23101 aaacgtccct cgcgcaaaac atgcacctca aagtcgggag ggagcgccaa aatagttttg
    23161 agggtggcag aaaaatcaat ccgagagaac tcttcaatag cataattcgg atagaggtgc
    23221 tctggtcggg gacttgcgga agcttggcaa gggatcggca cgccgctgga ggaaatgtgg
    23281 aggcgctgtg tgggcggtta gttacagtga atttgaaaca aaaggaagcg atagagtttc
    23341 cgacaggagc aggccggcct gggtagagga aggtgttttg ggagcggaga ccgcgttgtc
    23401 gcccgtgttt gcggtgccgg gagtcgggct gcatatcccg atcttggagg gtgttgccca
    23461 aaacggaggt gcgccttaag ggatgataac ccgtcgaaag attagacagg cttagcgggg
    23521 atcggtattt tcggacggtc gagatgactt ccagcgaccg agagaatctg ggcatggaga
    23581 ccccgcaagg gccagggggc tgcttcaaca ccacccattg gtcggtggtg ctgagggctg
    23641 ggcgctccga cacgccgcgg gcacgggcgg cgctggatca gctttgccgg cattattggc
    23701 agccgctgta tgcgtatgta cggcgggcgg ggtattcgcg ggaggaggcg gaggatttga
    23761 cccaggagtt cttcgcgcgg ctgctggcgc agaacagtgt ggcacgggcg gatccgatgc
    23821 gggggcggtt caggtcgttc ttgctctcgt cgctgaagca tttcctgatg aacgaatggg
    23881 acaaggcgcg ggcgcaaaaa cggggcggtg gggtgccggt gctttcactg gcattcgacg
    23941 cggcggagac gagttgcgcg tcggcggtgg ccccggggga cacccccgac cgagcttatg
    24001 accgtcaatg ggtcttggcg ctgctggaag tggtgctggg gcggctgcgc gaggagcacc
    24061 aggcggcggg gcgggaggga acgtttctcg ggctgaagga cacgttggcg ggagggcggt
    24121 cggagattcc gtaccgggaa ctgggagcgc ggttgggaat gagcgaaggg gcggtgaagg
    24181 tggcggcaca ccggctgcgg gcgcggtatc gggagttact gcgagaagag attgcaaata
    24241 cggtggcgag gccggaggag gtggagaaag agttgcgcga gttgtttgcg gcgttggctt
    24301 attgaggccg ttgcctgagt tgccggcggt gggaccgtaa ctttggagcg cgcttccggt
    24361 agtaagcagt gtatgaactg tatgatgctg ggtgggttga tgctgagttc ctcagagata
    24421 ctgcttatct ttttggcgct gctggtcaca gcagggatag cggcagcagt gattgcgttc
    24481 gttgtctggc tgacgcggcg aggagcggcg ggcatgccgg acaaggcgcc catccaacaa
    24541 ccgccgccgt caacctctgc gccagcaagg cggcaaggca ggaagttcgc ttttgtcgag
    24601 gagcgagggg gcaagttgcg ggttcgttgg tgggtctgct gcgtttcgat gggggtaggg
    24661 gtattggcga cggcgctggt cctcgagctc tttctgagga ttggcggctg gcggaacgaa
    24721 aaggtgtgga ttcccgccat cgtcgtcggg gcggggtcaa tctgcgcatg gttgatctac
    24781 gagttcagcc aggcaaggga agcgcgcgca acggggtggc ccgcttctgc gcctgcgtca
    24841 ccgatcccgg gaggaactgt ggctcttgcc gcctgtccgc gatgcggggc ggcgctgccg
    24901 gcggacgcgc cgcaggggtt atgcccacgc tgcgtgctgg gggtcgggct ggcgacgcac
    24961 acggaagcga cgggagaatc ggggccgcac gggactaagg tggttcagcc gccgccagaa
    25021 cccgcagaga ttgcgaagca tttcccgcaa ctggagattc tggaatgtct ggggcggggc
    25081 gggatggggg tggtctataa ggcgcgacag ccgaagctaa accggctcgt ggctttgaaa
    25141 atcctcgcac cggaaagggg tgcggatccg cagttcaccg aacgcttctt gcgggaggcg
    25201 caggcgctgg cgcggttgaa ccacccgaac atcgtaacgg tgcatgattt cggggaagcg
    25261 gagggacttt actacctgct gatggagtat gtggacggga tgacgctgcg gcagttgttg
    25321 catgggcaga agattgcgcc ggaagaggcc ctgagcatcg tgccgaagat ttgcgaggcg
    25381 ctgcagtttg cgcacgagtt gggggtggtg caccgggaca tcaaaccgga gaacgtgctg
    25441 gtggactggc aggggcgggt gaagatcgca gacttcggca tcgccaagat cgtggccgcc
    25501 gaccagccgc ggcaggcgat cacgcaggac caggtgatcg gcacgccgca ttacatggcg
    25561 ccggagcagg tggagaagcc gcagttggtg gaccaccggg cggacattta ctcgctgggg
    25621 gtggtgttct acgagatgct gacgggcgaa ctgccgctgg ggaagttcca gccgccgtca
    25681 aagaaggtgc aggtggacgt gcgcctggac gaggtggtgt tgcacgcgtt ggagaaggag
    25741 ccagagcggc gttaccagca tgccagcgag gtgaagacgg ccgttgagac gattgcggcc
    25801 acgcccggag cgccggcggc ctgtgcgccc gcggggccgc ccgtagtcat caagcgctgg
    25861 cgggacgcct ggatttggaa ctgggagtat atccagttgt tcctgatcgt gccgatagta
    25921 gccgccgggc tggctttgcc gttcctcctg ccccgtttgg gcctacgagc gctctggatg
    25981 ttcgccgccg cgttgggagg catcgcgttt gccatcacgt atgccatcgt gggccggcag
    26041 gtccgccggg cgcgggcgga ggtgtcacga accgagcgtg acgtggcgga gtgcctcatg
    26101 tttcggcggc cgttccagtc gcccggggtg gcggtgatgc acgaggcccg gctggagctg
    26161 attcccatcg tgggctcgcc catcattgtg ccgctggcgg acatcgtcgc agtcaaggag
    26221 gtgcggtggt tcaacgggca gcggcttttt ttcaagaggg gctttgtgat ggacctggcg
    26281 aatggccagc gggtgggcgt ggcagtggcg gaagtgttcg cccgccgctg gcgggccaag
    26341 ctgagccgcg gcacgctgcc ggagattccg gcgggcagcg ggcgggatca gagcctcctt
    26401 gcgtcggctg cggcgaagaa gtggatgatg gcggcgcgat ggacggcgcg ggtgctgggc
    26461 acgctgttgt ttgccttcta cgggctcttt ctcttcggcg aaggggtgcc gccaatcgga
    26521 tcgcagccgg agggggtgca gttgagtttc gtgggggtgg ggctgatgct gctgggattt
    26581 gtggtgggct ggcagcgaga aggagcggcg gcgctgctga ttgcgtcggg ttggacggtg
    26641 ttccgcatcg ctgagggtgg ttttgggctg ggagcgtttg aggcgccgct gctggtggcg
    26701 gcgttgtatg ggctatactg gtgggtgacg caggtgcgga agacgcacct cgcgttggca
    26761 gcggtcgcag cgttggtgat ggcgctaact tgggggcaac tgatttgccc gaccagcgtg
    26821 ttcgtgcggg ggatgatcat tgacgcggaa acgaccagac ctatcgcgga cgcggaactg
    26881 acgctggaag gcagcccgga gaaacggggc gaggagggaa tgcatcccaa cacgcgttcg
    26941 cgcaaagacg gacggtttac tctctacgtc ggctggtatg cggagcaaaa gaagatgacc
    27001 atcgcggcgc cgggttacga gacgctcacg accaatctgg gaccgcgggc gctgggccag
    27061 cggaaggtga gccggactta cctgttgcgg cgagtgggca acccgaccag cggccagagc
    27121 gagggcgcgc ggcgggagtt catgcggttg gtggcggcga aggacgcagt gccggtcgaa
    27181 gggatgccaa cgaccagggg cgcggcgcgg tgggggcgcg gttggagggg gtgccgaatc
    27241 ggtagaacac ggtgctgaag tgcgcggtgg cctcggatca gatcacggtt caacagcaga
    27301 atgagcagtt cggctagcgc tggaatcgag ccagaacctg cggcgtctgc ggatttggtt
    27361 caggtggcat tgcaaacgcc ggctttcggg ccgcccggaa cgaggtttgg cccagtgacc
    27421 gggcaagtgt tgaatgagcc ggacgcccgg ccggagaagg aggtcttggc attggcctcc
    27481 gggggcgacc gcgagccgcc ggagacaggc aaggcactgg aaacgcccac cgattacccc
    27541 ggatgaggaa atacggagca tgccggagtg ctggcgattg agcagttcgg ctttgtgttg
    27601 gcctgggacg caagagggcg cgggaggtgc ctggcggaat tgcgccccgg ggcagcgggc
    27661 ggcgtgttct tgttcctgcc gcgactgact caatttgagt cactacagtt gtggcgcggg
    27721 gcttgggcgg cgcggttgcg cgctcaggaa ctgttttccc aggcgcgttc tcggtttccg
    27781 gcccgcggcg gcactaaacc tgcttaaata agcctgtcaa ccgcaattgt acgaacgctt
    27841 tcgcatgagc ttattgcgtc catgtccgcg cgggttcggc cgggcatatt ggtttttggc
    27901 cggacgggaa gggccaccgc catgcccggc catggccaag agttgcttcc gttccggccg
    27961 ggaacaccgg cttgcttcgt ccaatccggg cagtacaccc tatgaatact aacccaaaga
    28021 caccctctcc gccatgcacg gccgtgctgg tggacgatga tttgaccagc cgctggctgc
    28081 tgcgcggcgt gctcaaacgc ctgggcttca cgctcctggc ggaagcctcg aatggggaag
    28141 aggcggtcgc cgtggtgcgc cgtaccaaac ccgacatcgt gctgctggac gtctgcatgc
    28201 cgtttcaaac cggcccgggc gcgctgccgg cgattgtcgc cgcccatccc cccgcccaaa
    28261 tcgtcatgct tacctcgatg gccgacgaag cgaccgtgcg ctactgcatc gaacagggcg
    28321 ccgcggagta cctgcgcaag gatacgcccg tggaggaaat cagccgggtg ttgggcggat
    28381 tgagcgagaa aatcgcggcg caacgcgagc gggaggtgcg ctgtgcgtga gcctaaaccg
    28441 ccgcgctttg acctcgacac gctcctggcg gagattccgc aggacgaggc ggtctgccgc
    28501 acggccccgc ggttgctttc ccaggatgat atccgccggc tgatcgagga gagccgtcgg
    28561 cgcaaggagc gggccgcccc gcgcgtttcg ccgcatgaaa agtgaagcct ttctgaaact
    28621 ggtcgtggac cggggcctgg tttccgccga gcaggcccgc accctttcgg cccggcatgg
    28681 caaggtggcg ctggcaatac tggaggagtt gattgaccgg cacgaaacgg ccaaagtgga
    28741 gctatgccag atgtacggtg atgccctggg cgtggcgtac ttggacctga cccgcactat
    28801 catccagccc gaggtcgtcc ggctcctgcc gcgggatttc gccgtcaagc accagatgat
    28861 tgcggtctat cgcttcgccg acagcgtcac cgtggccacc gccgaaccat tgaatcccac
    28921 ggtgctggac gacgcggcgc gactgctgtc cttgccggtg aatgccgtgt tcagcctgcc
    28981 gggcgaaatc aaggatgcca tcgaggtgga atacaccacc gtcacggttt tgcaggaaag
    29041 ccaggcgcaa atggggctga agcacgtatt cgtggaggaa acggaggcaa caccgcagcg
    29101 cctggcggga gtcgcggccg atcaaagcat cgtccagttg agccggcagc tcttcatttg
    29161 cgcgctgcgg gaaagagcca gcgacattca catcgagcca tcccaacacg aggtgcgcgt
    29221 ccgcttgcgc attgatggcg tcttgcagga gcgcttccat ctggaccgcc gtttgctggc
    29281 cccgctggcg tcccgcctca agatcatggc cgggtgcgat attgccgagc ggcgccggcc
    29341 gcaggatggc cgcatcgctt ttgaactcgc cacccgctcc ctggatgtgc ggttttcatg
    29401 cgtgccgggc atggacggcg agaagattgc gctgcgcctg ctgggaaccg tgcgcggccg
    29461 ccccattccg ctgctggagg aattagattt ttcccacgcc tgcctgggca cggttaaaca
    29521 aatcgccgcc agccccagcg gggtgttctt tgtcaccggc ccgacgggct ccggcaaaac
    29581 aaccaccctt tattcggtgc tcaagctgtt gaattcgccc ggcgtccaca tcgtcaccgt
    29641 ggaagacccg gtggaatatc gcctgcccgg caccacccag gtgcaggtca acccggacgt
    29701 ggacctgacg tttctgacgg ccctgcgcag tttcctacgc caggatccca acatcattct
    29761 ggtgggcgaa gtgcgggatc tggaaaccgc caaaatggtt tcccaggcgg cccttaccgg
    29821 ccacctggtg atgtccacgc tgcataccaa cagcgccatc caggccatca cccggctcgt
    29881 cgagatcggc gtcgagccgt ttctcgtggc ctccgcgctc gccggcgtga gcgcgcaacg
    29941 cctcgtccgc aggatttgtc cgaattgccg ggaagaaatc ccgatgcccc gatccgccat
    30001 cgaacgttat ttcggcccgc tcggagaccg cacggtgaac atgtggcacg gacgcggctg
    30061 cgatgcctgc catcggaccg gctacagcgg gcgcctggcc attcacgaag tcttcatgct
    30121 ttccgaagaa atccgccgcc tcatcgccga aaaagccacc cccccggaac tccagtccct
    30181 ggccgaacaa tatggttacc gccccatggt ctatgacggg atcaaaaagg tgctgcgcgg
    30241 cctgaccacc ctggcggagg tggagcgcgt gacggccatt cccgaagagt agcaaccacc
    30301 ccgcgccgcc gccggcgcga aattgcagga ttgcctttgc ccgctccggc gggtatggga
    30361 acggctaccg atgaaaacca tctgcccgtt agcaatgctg ttccttgcgt gcgccctaac
    30421 cctggtcggt tgcgccacgc gcccgccttc tccgcccgaa tcccaggggc cgcccggagc
    30481 ccggacgccg atgggcctga tcgcggccac ggcggatctg gccgccgccg gcgccacacg
    30541 cctgcatccg aactatatct gggccaccct caaagaccgg aaacaggatg cggccctcaa
    30601 caccgccttc gaacgggcgc tgaccgaggg caccccccgc gcgcaacagg cgttgctgcg
    30661 cgcgctggaa ttgaagttta tccacgaccc gcaaacctcg ggactggaca tcctgggccc
    30721 cgaattaagc caccatttcc ggacgttcgc ctggcaggcg gaggatttcc ccggcggccc
    30781 gcccggcccg aatgaacagt ttgccgacgt gatggtggac gtgctggacc tcgtgcgccc
    30841 ggagcgccgc gccaacagca cgcgcgacgc cgtggtgctg cgcgaggaag ccaccgaggc
    30901 ggtttgggaa tacatgaccc gccaatgggt gccggtcccc gggcaggaaa actggaagct
    30961 gaaccgccat gcgcgggagt cgttcatccg catgcgcgaa caggcccggg ccgagggagt
    31021 ggacctggtg atactctcgg cgcaccggct gcgcaaaacc gccgaagcca acgccgcgcg
    31081 cgcccgcaac ccgaatgccg tggcgagttt ctccgcgcat tcgctgggcc tggcggttga
    31141 cttcgatatg agccaccgca acgacatcaa gttcagcggg ctctcaaccc gccccatgtc
    31201 ggaactcgtc cgcatgcgcg aatcgcccgt gcataaatgg ctgctcctgc gcggcgagga
    31261 gttcggctgg tatccctacc agaacgaacc gtggcattgg gagtacaacc cgcccggctt
    31321 ccgcgaaatc ttctgggccg atttccccgg cggcgcgccc gagcggggca tcatcatcga
    31381 agaaccgtga aggggcggca acctcgctca gcggctggat aacaggcata cccgcggctt
    31441 ggagaagcgg gcctgcgctc cgcccggttc ggccgcgcgg gtccgcgcac gcgggaaggg
    31501 gcagggccgc acggaagcgg cagccggatt gaaaacgccc gctccttggc cgttggggcg
    31561 ggtgcggcgg ggctagcggg gccgtgagcg cgggagcggc agcgggaggt agccgatgta
    31621 atgatccacc gtggttgcgc ccatgacgaa cggagcagtc tccgtgatgg taaaccccag
    31681 gcgcttgtac cactcgacag cctgcgtgtt cttgaccatt acccccagcc aaatgcgatc
    31741 cgcgcccaac tcgcgggcgc gttcttcggc gcaggtcatc aggcggtggc caagcccatg
    31801 cccctgccag gccggcaaaa tgtagagttg atgcacgtag aacttgagct ccgcgcgggc
    31861 gtgaaacagc ttggcgtagc cggcaacccg gccatccacc tcggcaacca ggcccgtcac
    31921 atccggatcg gcgaacagcg ccgcgagcct gagttcggaa tactgcgtgt gaaggtaacc
    31981 ctccaggtcc gtttgcggga tgaatgagcc gtaggcttcc aaccacgttt cgagcagtag
    32041 ctgctgcacg gttgccaggt cggctcgggt ccagcgtcgg gtggagatgt tcatggggtg
    32101 tcgtgcaaga aatcttcgct ccccgcggcg gcgaggaaag cgggagttgt tctgaagcgt
    32161 cgtccgttgc cagccattgc tcgatccata gtgccgcctt gcgacggaat ggcaattcca
    32221 aaccttgcgg aaccaaccgc aaaaaaatgc gctcacgcca gggcaaccgg cggacgcagt
    32281 gaatccgtcg gcgggaaagg gccacgacga aagagcaaaa cccggaacaa cggaatcgct
    32341 gaaaacgagc gatacagggg ggtgaactct gttgcggcaa gtgaaggcga aaacgcctgg
    32401 cggctctcgc acggcggcgt aattccgggc acgagccact ttgaccggaa tgcaactttg
    32461 caactatctc cgcctaatcg ggcgaatact ccgcccacga tgagggcaac gcaagcagcc
    32521 gcctttcggc aaccccc
//
LOCUS       DAOPQG010000032        31297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1882_length_31297_cov_14.563248, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000032 DAOPQG010000000
VERSION     DAOPQG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 31297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31297
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1882_length_31297_cov_14.563248"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1573
                     /locus_tag="PKI07_05325"
     CDS             257..1573
                     /locus_tag="PKI07_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50507.1"
                     /translation="MNRRRGKPARNLLVLCLLSLVWVADSSGASDRRLDASDITKPDF
                     FPILPWSPYHGWRAPFITHRPSGLESIADCQFNLAGFVWAQDLRRCRKLGLGAILVPA
                     AAEVTSPGYYREWKKLSDAEIEGRVLRMVRAAGRNPAVKGFYIMDEPGIMDFPALGKA
                     VAAVKKHAPGKLAYINLFPNYATLGAPNMSQLGTSNYTEYLERFVAEVRPQVISYDNY
                     MVQYSEDLREPKKAASYYRNLLEVRRVARAHQLPYLNIVSANQIRPHTPIPSPANLQF
                     QAYTTLAAGYRGITWFNYYGPGYKYTPIDPAGRKTLTWVYLRDINAQVAALAPVMSRL
                     TSTGVFFSAPAPVDGLPLLPGKLVESVACDAPVMVGEFRHADGEAYVMVVNLSLERSA
                     RFTFQAASPRETVQVISAGDGSRSDYDLKNGLWLTAGQGALLALGR"
     gene            complement(1562..2230)
                     /locus_tag="PKI07_05330"
     CDS             complement(1562..2230)
                     /locus_tag="PKI07_05330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HOC50508.1"
                     /translation="MKPSVPARTAVLVIDVQRGLFGSEPPPFAAASVLGRINAVTAQA
                     RRAGVPVIFIQHDGDPSEPDLAPFRAGWELHPHLQVRPGELILRKTTCDAFYRTALEA
                     ALRSRGVTTLLLTGFATDFCVDATLRNALSRDFHVIVVADAHTTTDNPLLNARTVRAY
                     YNWVWANLAAHGRVSVLKAAELRFPPAPKSAAPAGRSRPRRRHGPEAALPPGGDGGPD
                     SSAP"
     gene            complement(2241..4229)
                     /locus_tag="PKI07_05335"
     CDS             complement(2241..4229)
                     /locus_tag="PKI07_05335"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOC50509.1"
                     /translation="MQDERKTLDDRNQMTDLERLRHSCAHVMAAAILRLWPEAQFAAG
                     PAVENGFYYDVDLPHRITPEDFPAIEAEMKKEIKANHRFEKIVVTREQAIKDSESGRL
                     GGLSERPGNPSKFKLGNLAEIPAHEPISYYKTGDFIDLCAGPHVMRTGNIGAFKLTTV
                     ASAYYQGDEKNPQLQRIYGTAFKTKAQLDAYFQMLEEARRRDHRKLGAEMGLYTIDTE
                     YTGPGLPLWLPKGTAILEELEKLAKETEFLAGYVRAKTPHIAKEKMYLTSGHLPYYAE
                     SMFPPMELLEEEEHSRKRDLEAQLEALKQAAPGGNGEPPPGPEGSAAHREYDQKIHTL
                     TEQLRALGTRYYLKAMNCPHHHRIFAAVPRSYRDLPLRLAEYGCCYRYEQSGELFGLM
                     RVRSMNMNDAHIYCTPEQFAQEFNAVNEMYLKYFKIFGIEKYLMRFSTHGREGLGRKY
                     VNEPELWLQTENMVRDVLKNSGINYVEVANEAAFYGPKIDVQVWSVIGREFTLATNQV
                     DFAVPQRFGLVYRDRDNTEKTPLCIHRAPLGTHERFIGFLIEHYAGNFPLWLAPEQVR
                     VLTIGEEAPLVNYAQALVRELRAHLVRAEGDYSNDKINGKIQQAEQAKVHTMLVVGPR
                     DLAANAVSVRVHGRGSLGAQPKAEVVAGILAAIRERRP"
     gene            complement(4361..5920)
                     /locus_tag="PKI07_05340"
     CDS             complement(4361..5920)
                     /locus_tag="PKI07_05340"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOC50510.1"
                     /translation="MKKRPAPAPPATLPPPLRGYLYFTAAVTGAAIMIIEILGAKMLS
                     PYIGLSHFVWTAQIAVTLVALACGYYAGGRLADQSPQLARLYWAILAAALYLVLTVHL
                     CEPLAYWCLDFSLPVGSLLASALLFFIPLALLATTGPYLVRVITSTVAGVGGNVGRLT
                     AISTLGSLAGTLLIGYLLIPLLPNSRTMTGTALGLMLVSAGYFLFTRRRGAAALLLLL
                     ALGAAAAGQRSLSSTPAYAHVVELFRGNSHFGQLQVVDARAYPYRMYLNDNLVQNTYD
                     PERKLSVSLFTCALAGLADVYTPRLADALCIGLGVGIVPMELAHRGVRVDVVEINPAV
                     VPVAVRFFDLQTNQIHLTLDDGRHFLNRCRRQYDAVILDAFLGDSSPTHLFTREAFSA
                     IRRVLRPGGTLVINSFGSLDEGRDFFSTSLHHTLQAVFPSVRAHASGDGAIFFVASDQ
                     PELAFRREPDLSRVHPYILREVQSLYAGVVHPAPAHGRILTDDFNPAEFYDARNREDF
                     RRKLALAARQM"
     gene            6301..6534
                     /locus_tag="PKI07_05345"
     CDS             6301..6534
                     /locus_tag="PKI07_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOC50511.1"
                     /translation="MADVANKYPDNAPGKYYVDNQCIDCDLCRETAPDNFMRNEDGGY
                     SYVSKQPATPEEEERCKEAKEACPVEAIGDDGA"
     gene            6756..7274
                     /locus_tag="PKI07_05350"
     CDS             6756..7274
                     /locus_tag="PKI07_05350"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC50512.1"
                     /translation="MAKLVVLSAGMTGRTHELQVERTTIGRVEDNTFTIAEPSVSSHH
                     CEVLLRGSDIVVKDLNSTNGTYINGEKITEAIIKPGQILRLGQVEVRLETDAPAAPAK
                     KKMDQTLVMQRGVSLNDLEQSAHGGGFDTKGTGFTKKTDRGSKVFWIVAALVGLIIVV
                     GLLYALTTISGK"
     gene            complement(7276..8283)
                     /gene="hemB"
                     /locus_tag="PKI07_05355"
     CDS             complement(7276..8283)
                     /gene="hemB"
                     /locus_tag="PKI07_05355"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOC50513.1"
                     /translation="MSFYPAGSFPAHRPRRLRQSPRLRQLVRETSLNAGQLVLPLFVR
                     SGRKLRRPIAAMPGVYQLSPDELLHEAGRALEAGIPAVLLFGIPERKDARASGAYAAR
                     GIVQQAVRLLKRELPELLVITDVCLCEYMAHGHCGLVQRHKSGARILNDPTLKLLART
                     AVSHAEAGADMVAPSDMMDGRVRALRAELDRHGFPDTPIMAYAAKFASAFYGPFREAA
                     ESAPKFGDRRSYQMDPANASEALREVALDIQEGADLVMVKPALSCLDIVYRVKTAFGY
                     PTAAYAVSAEYSMVKAAAAQGWIDERAVVLESLLALRRAGADIIITYAAVDAARWLKE
                     G"
     gene            8440..9249
                     /locus_tag="PKI07_05360"
     CDS             8440..9249
                     /locus_tag="PKI07_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50514.1"
                     /translation="MKNLIGVIAFILVLVCLGLGIGLITIKKQATDQQHDLTEKNIAL
                     SNRLVQANDELDQQKQTNALLENDREERKKAFEALTNNYTQALISLAQTANTLAQTEA
                     ALKSSQEETARRDAKIAELEEQNQALDKRAADLNTAITDLTRQIEETKRKLASSEGEK
                     DFLNKELKRLMAEKAELERQFNDLAILRAQVSKLKEELSVARRLAWIRQGLFASPDQK
                     GAQRLMRSPSATQPKAPPPNYDLNVEVTADGSVKVIPPLTNRPPATNPPAH"
     gene            9319..9666
                     /locus_tag="PKI07_05365"
     CDS             9319..9666
                     /locus_tag="PKI07_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC50515.1"
                     /translation="MPTYEYVCEKCGHRFEKFQPITAPPLTACPKDLCARQPWGKGKV
                     KRQIGAGAGLIFKGNGFYITDYRSEKYKEAAKKDVPPAAASSSTEKPATSSGGKPAAK
                     PAGKSASKAAARN"
     gene            9772..11166
                     /locus_tag="PKI07_05370"
     CDS             9772..11166
                     /locus_tag="PKI07_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50516.1"
                     /translation="MKIQVKAVTGLGMALAMASALWLPAGPCWAGATQLPANLVSARS
                     QSGQFVVYAGSTPGSIPPVLEPAKNQGFIRLEATLVAVSCERIKQLLTRELDDPAPWR
                     GTIYLVLYPARGASDPITITTERFKDESHYRVDMPDVVERIRYVRAVVQVLLIEMANR
                     AGWAQAAEVPAWLSEGFTQLLLAANEVEIILPAPSESTSGLNLSTMTVRSRKESLEDQ
                     VRKKLRGRAPLTFEDLSWPTEEQRSGEAGEVYSGSAHLFVGELLRLPEGRAALRGMLT
                     RLPQHYNWQFAFLKAFSPRFERPLDVEKWWALSRAQILGREPATAWSLEESWRKLDQA
                     LRAAVLVRAATNDSPLRADVPLQTVLREWEPQRQILAFDHALRQLELLRPRLAPELAG
                     LVEDYSQAIKAYTQQQGRGGTVLPASRKASQQRAVEAAIQQLDALDVRRQAMLSPSLA
                     PEAVPLPAASAPPP"
     gene            11231..12577
                     /locus_tag="PKI07_05375"
     CDS             11231..12577
                     /locus_tag="PKI07_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOC50517.1"
                     /translation="MKPRVRFAPSPTGFLHIGGARTALFNWLYARHAGGTFILRIEDT
                     DAARNTQAAVEVILNGLRWLGLDWDEGPLSGDPAGPSRGDYGPYFQSRRRDIYQRRIE
                     ALLAKGLAYEADGTIKFKMDRAPILIPDLVVGDVRRELTDREQADPDFIIVRSDGQPV
                     FHFVNVVDDLEMGITHVIRGEDHLSNTAKHIALFRALDADPPQYAHIPLILNSDGTKM
                     SKRDQGASLMTYGEEGYLPEALVNYLCLLGWSPKGNREKMPLTEVIAAFDLPQILRHN
                     ARFDLAKLVWLNGEYLRELSDERFYELSAEALRRGGLELARYPDAYVRAALETCKGKV
                     KLFSELPAYAGFYFTDEIKYDPAAAEKEFTPENRPRVDKLRAALAKLEPFEPDPIGAT
                     LKAVAQELGVKTGVLVHPTRLACCGNTAGPSLYHLLAILGKDRALARLDQALARMV"
     gene            complement(12588..13085)
                     /gene="purE"
                     /locus_tag="PKI07_05380"
     CDS             complement(12588..13085)
                     /gene="purE"
                     /locus_tag="PKI07_05380"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOC50518.1"
                     /translation="MKQTPKPRIGIIMGSDSDWPTMKAAAEACAEFGVASEERVISAH
                     RTPRDLERYATTARARGLQVIIAGAGGAAHLPGVAAAFTTLPVIGVPIESKALKGLDS
                     LLSIVQMPSGIPVATVAIGGARNAGLLALQILAVGDAALQKKLLAFKARLATDSRAKN
                     RNLAG"
     gene            13277..13858
                     /locus_tag="PKI07_05385"
     CDS             13277..13858
                     /locus_tag="PKI07_05385"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOC50519.1"
                     /translation="MTTANKITILRILLIPVFVVAVLYYGQGGKSVHWWLAVLCFATA
                     SICDGVDGYIARRFNQRSELGAMLDPLADKLLLASAVIVLSFDHRPYLEAVPLWLTGT
                     IIGRDMLLLIGLLVIRLMVGKFTIRPRVVGKVATVLQMAVVLWILLRWPQRWLLFWTI
                     SAAVCTGASGLLYVWDGSRQLSAHPSASPKPGS"
     gene            14151..15167
                     /locus_tag="PKI07_05390"
     CDS             14151..15167
                     /locus_tag="PKI07_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC50520.1"
                     /translation="MLTRRGCSSKSLALISHLDTVFPPEEEERNNFRWQLEGDRIFGP
                     GTHDIKGGSVMMWLVLSALQVQFPKLFANITWKLIWNSSEECYSPDFAEVCRTHFDDG
                     TLAALVFESEGRLDNIPQLIVARKGRGTWRATVGGRGSHAGGKHAHGVNAIVQLGRTV
                     QRIAALTNYERGLTVNVGTINGGTVLNRVPHEAVAEGEFRAFTPEVFMQAKQDLLALA
                     GPGEVHSSADGYACKVQIEILTESAPWPRNPGTDGLFQLWKQTADELGLALNCEERGG
                     LSDGNLIWDVVPTLDGLGPWGDNDHCSERSPDGSKLPEFVEASSFVPKAVLNIAALQK
                     LCGS"
     gene            15314..15853
                     /locus_tag="PKI07_05395"
     CDS             15314..15853
                     /locus_tag="PKI07_05395"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOC50521.1"
                     /translation="MTGPRSVRNLALIGFMGSGKSCVGRMAADLLHFTFLDTDQVIEA
                     RAGKRISDIFAQDGERGFREWERRIVEELTRREKTVIATGGGLPIDPDNLASLKTHSL
                     VICLWASPETIWERTRGHSHRPLLNESDPLTKIRQLLAAREPYYRQADVLVNTEMRSL
                     REVVQHVLHQFYLVRSGHR"
     gene            15850..16884
                     /gene="queG"
                     /locus_tag="PKI07_05400"
     CDS             15850..16884
                     /gene="queG"
                     /locus_tag="PKI07_05400"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOC50522.1"
                     /translation="MKEALRQRARELGFDDCRVTTASPPETAPYFQQWLAAGRQGRMA
                     WLERSMRKRTDPQVVLPGARSIITLAASYHCRGSQTGSVARYARFDDYHNILSERLEI
                     LTDFVSQLGESGTRSQWYVDVGPVLERDVAQRAGLGFIGKHTNLISRRLGNWFFLAEI
                     ITTLALEPDPSERTRCGSCRRCIKACPTAAIIAPFQLDARYCISYLTIELKGSIPVAL
                     RPAIGNRIFGCDECLLACPWNRFAREGQIMKTYARPGFTQLDLLELLTLGESEFTRRF
                     AGTPIQRVRRRGLVRNVCVALGNTGGEPALAALHKATSDAEPLVAQHAQWAVEQVTAR
                     ISTHRGVLPD"
     gene            17127..18269
                     /locus_tag="PKI07_05405"
     CDS             17127..18269
                     /locus_tag="PKI07_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50523.1"
                     /translation="MKKLNVGLIGCGFMGRTHSNAARRVNNFFDLEYQPVLKAVCDLD
                     AARAQAFAAQWGYESCETDWRKLIARQDIDMVDITVPNDLHREVAVAAAKAGKVILCE
                     KPLARTGPEALKMVQAVERAGVPNFVSYNYRRIPAVTLAKQLIDEGRLGRIFHYRAKF
                     LQDWTISKDLPQGGPALWRLDAKVAGSGVTGDLLAHCIDTALWLNGDLEQVNAMAETF
                     IKQRKHTVSGKVEKVGIDDACAFLARFKNGSLATFESTRFARGHKALYTFEINGEHAS
                     LFWDLHDLHRLQYFDHRDAGRVRGWRSIHVTDGDHPYMGKWWVPGLQIGYEHSFVHQL
                     ADFLQGLATGKSARPTFRDALQTQYVCDAVLKSAKTRRWEKVPKVK"
     gene            complement(18412..19890)
                     /gene="rho"
                     /locus_tag="PKI07_05410"
     CDS             complement(18412..19890)
                     /gene="rho"
                     /locus_tag="PKI07_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOC50524.1"
                     /translation="MPRPKKKAKSKAAKEVVAAPLNGNDAGVSVPLDTTAEAPAEALV
                     PPSTPVEPLVEEPPRTKHAPEREKEPGAPASVNIAKLQAMSMPDLNQMARDLEVENFG
                     TMRKHEVIFHILQKNAERAGVLFSEGVLEVLPEGFGFLRSQSFNYLPCPEDIYVSPSQ
                     IRRFDLQTGNLVAGQIRPPKEKEKFFALLKVEAVDQEEPDKAKDKILFDNLTPLFPNQ
                     RFILETTPDELSTRVLDLVCPIGKGSRGLIVAPPRTGKTVLMQKVANAILKNNPETYL
                     FILLIDERPEEVTDMERTCRGAEVISSTFDEPPERHVQVAEMVIEKARRMIEHKRDVV
                     ILLDSITRLARAYNTVQPHSGKILSGGVDANALHKPKRFFGAARNIEEGGSLTIIATA
                     LVDTGSRMDEVIFEEFKGTGNMEICLDRALVDRRIFPSINIERSGTRKEELLYHPDEY
                     NRVVILRRALTGVPPVEAMELVLNKLKKTHSNAEFLMGVSVS"
     gene            complement(20155..21924)
                     /locus_tag="PKI07_05415"
     CDS             complement(20155..21924)
                     /locus_tag="PKI07_05415"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOC50525.1"
                     /translation="MKTILFVCTGNVCRSPMAEGIFRQFIRERGDYRAVSAGLGAADG
                     QPPTPHAVAALKELDIDISGLRSQALTAELVSQADYIFGMTLGHVETIATLYPPAREK
                     TFLLREFDEQLGPGEIDIRDPIGGSYAIYVDCRDQIKRGITSLLRFIESGATEPRTSD
                     FEGHQKKGTFMEKRIMPADYRLQAVDPEVAAAIKQEVRRQQENIELIASENFTSPAVM
                     EAQGSVLTNKYAEGYPRKRWYGGCENVDVIEQLAIERARKLFGAEHANVQPHSGSQAN
                     MAVYFAFLKPGDKLLTMDLTHGGHLTHGNKANFSGRFYEVIHYGVRKDDERIDYEQLA
                     QMAREHRPKMITVGASAYPRIIEFERLGQIAREVGAYLLADIAHIAGLVAAGLHPNPV
                     PYADFVTTTTHKTLRGPRGGLVLCREKYAKEIDSQTFPGIQGGPLMHVIAAKAVCLLE
                     AMQPSFQEYQKQIVKNAAALAAGMKQNNFRLVSGGTDNHLMLVNVGARGATGKECQAA
                     LDLAGITVNKNTIPFETRSPFLASGIRLGTPAVTTRGMAESEMSAIADMISEVLLDLK
                     NVEVSEKVRQRVHELTARFPLPY"
     gene            complement(21981..23045)
                     /locus_tag="PKI07_05420"
     CDS             complement(21981..23045)
                     /locus_tag="PKI07_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOC50526.1"
                     /translation="MIRIGTSCLVTVWLVLCGGLAGLLLTVATAEETSGPDCKEVYDL
                     IRSHLVGITETQLNEAVVRGLVAELSPRVVLITNGKAPRTMAGPRVSKAEVFQGDILY
                     IRVAQVGEGLSEAIARTWSNPGSANGVNGLILDLRYAGGDDYAAAAAAADLFVKKEQP
                     LLDCGNGIVRAQTKSNAICVPVAVLVNRQTTEAAEAMAAVLRKTGTALLLGGKTAGQA
                     QVTQEFPLKNGVRLRIATAMIQLGDGSALTTEGLTPDIVVAVNPAEEQKYFADAFQTL
                     AKTNLPVGTGIAGATPATRRPRLNEAELMRERHEGLSEASLNAPRESEPEQPIVRDPV
                     LARALDLLRGLALVRPMIRS"
     gene            complement(23165..24370)
                     /locus_tag="PKI07_05425"
     CDS             complement(23165..24370)
                     /locus_tag="PKI07_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOC50527.1"
                     /translation="MPSIPIIMPQLGESIAEATVVNVLVPVGDHVQTDQDIIKVETSK
                     ATMNITSPCPGRMERFLAKPGTSYPVGAVLGYLEASPEDATRLGLDLDSSADDGGDES
                     EFDLSRRELAGASHKGVQPTVRGLPVPANAEGASYMSPRMKARMMELGLHAADLAGLP
                     GSGAAGRVTIQDFERFIANLERHKLTQASPMRVAVADAMRRSWTRPLATVALPVCFDP
                     LLNHRKMCDPKPGPALYALRALALALAENSTPAGRLIGNKIVHPTAIDIGFAVEAEEG
                     VLVPVIRSAEKTPLKDLVKRYDELIKLAHERKLPTDVTGGSIATVTNFGTFGLTWATP
                     IPLPEQTLVLGMGAARPVPAWDTSKAQFVPAMEANLTLSFDHRVLDGGAAGRLLARVA
                     HLLSSPDKL"
     gene            complement(24387..25352)
                     /locus_tag="PKI07_05430"
     CDS             complement(24387..25352)
                     /locus_tag="PKI07_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOC50528.1"
                     /translation="MSLTYLDAIREAQARALADDPRVFIYGQDIGTFGGAFKATKNLA
                     QRFPGRVIDSPISEDAMLGLAVGAAIQGMRPIIEMQFADFSTLGFNQIVNQAATLYWR
                     TRVPCPITVRLPCGGTSGSGPFHSQSMEAVYAHYPGLIVMTPATVEDAYSMLLEAIAL
                     DDPVIFCEHKYLYYHLKAAQLPVEAMPTGKARIARAGRDMTLVAYSAMVHEALAVADE
                     LASEGAEIEVVDLRSVKPLDTDTVMASVARTGRLLCVGEAWPWGGVTAEVIARVTSEG
                     FGLLDAPPQRLNAKETPVPYHPSLWAAHRPTAHSIAAAARRLLRL"
     gene            complement(25349..26452)
                     /locus_tag="PKI07_05435"
     CDS             complement(25349..26452)
                     /locus_tag="PKI07_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOC50529.1"
                     /translation="MFQDTKETLPEDTEADTVPCMAHAQLSHHAVSGDSSFRDLYVKA
                     YRVMLLARVLDDKFASLYRMGKIHGGVFLGRGQEALSVAAGLALRKGDIFAPLIRDGA
                     GRLAFGEPILDAVRTYLGSPLGPMRARDGNVHRGRPREGVFAMISHLGSMISMVNGAL
                     LAHRFKGISDTVGVASVGDGATSTGSFHEGVNQAAVEKLPLVLIIANNQYAYSTPTER
                     QFACASLLDKAAGYGIQGEAVEGTDLSACLRVIGGAVNRARAGNGPQMVVAQLLRLCG
                     HGEHDDAGYIDPNLKQSPVGRDCLKVAESFLVEEGWVDTPTLAAWRNEAVQETEASVA
                     QVQREAGPDPYKEDWSALASKHLSEGYDQGCPP"
     gene            27117..29609
                     /locus_tag="PKI07_05440"
     CDS             27117..29609
                     /locus_tag="PKI07_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC50530.1"
                     /translation="MNTPASQVLVVEDDPRLPEVLASLLRDENITLVTATNAVAALRL
                     ARTRPFDLILLDLGLPDANGFELLRQLKESPATQPIPVIVLSAWNSSSDKLRGFELGA
                     VDYLTKPFEGGELRARLRAALRTKHLQDELTQANHELLAARLAAETSARAKAEFLANM
                     SHEIRTPMNGIIAMTGLLRETPLTSEQRGYLETIYSSGESLLTIINDILDFSKIESGK
                     FELESQPFDLYGCAEEALDLLAPKAAEKKLELVCMWADEVPERVCGDVTRLRQVLVNL
                     LSNAVKFTAAGEVVIEAEVLSAPLRAEKNREPWQLHFRVRDTGIGIPVDRMARLFKSF
                     SQADASTSRCYGGTGLGLAISKRLVELMGGKMWVESVLQKGSTFHFTLPLRTASDQPP
                     TPAKTPPPQLANVRVLIVDDNATNCRFLSAQARKWGMKPRYTQRPLQALEWLRAGEGF
                     KLAIIDQDMPDMEGIKLAAEIRQLPKQGNLPLVLLNPPGFRVEGHDGANASPVNGLVK
                     PVKPGRLQELLVRIISGAQPAAPRAQSSPKADTTLAHRLPLRVLLCDDNQINQKVALR
                     LLQQMGYRADLAVNGLEALAALDRQPYDLIFMDLMMPEMGGLEAARAIRERQQRPAQF
                     PHYKSPIIIVAMTASAMPGDREKCFAAGMDDYLAKPVRLEDVRAIVERWGPVAGTAKS
                     EASAAAKTPVSAAAKEPASPAGSPPVGSSAPVDMARLLDFTDGSPENLRELVTLYLDQ
                     TSAQIEQIEAAVRDGKAQEVRRLAHSCAGASATCGMTNLVPLLRELERLGAENVLANA
                     SELARRAGIEFLRIREFLEAYLVEHASLAARP"
     gene            29612..30832
                     /locus_tag="PKI07_05445"
     CDS             29612..30832
                     /locus_tag="PKI07_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC50531.1"
                     /translation="MKKLLIIEDDQVVANIYRHKFAVEGYQVDVALDGLAGLEKVRLF
                     RPDVVILDLLLPGVSGVEVLKQLRAETEYAKLPVVVFSNTYLSSIMQEAWKAGATLCL
                     SKANCSPRQLIDGVRNALFPNDASATLFAPPTAAAGAIVSSPLTAESPAAETHPETRD
                     RDAAFQADLRNSFITSFPATLATLRSLLRELNQAGDETARLKPLGELYRRIHALTGNA
                     GIAGVPHVARLSDALEALLKELCETPRNINASTLRTVALAVDGLAPLGEQSVPPNQPQ
                     TSAPRVLVVGHETLSRRALVYALEKAKLDSVNIDDSIAAYELLVGNSFDLIFLDVDLP
                     GLNGFELSARLRALPAHQATPVVLVTSLSDFENHANASQNGGNDFIGKPFLFIEVAVK
                     ALAHVLRGPLFAVQ"
BASE COUNT         6104 a         9096 c         9574 g         6523 t
ORIGIN      
        1 ccctagcggg ctttgatttc gaggcgccgg aaaggtaagg cgtgcgaggc ggcttgctta
       61 tccggaccag gtttgaagag cgtttgtcat ttcggtcggc ccgggcggaa agtggccccg
      121 ggttcggcaa gcattttgac gttcagctca gtcacttgcg gggcagggaa tgatgacaac
      181 ccggttcccg ggcttgacgt ccggctccgg gcgcggccag attagcggtc ataaaacctg
      241 gctttctcga ttaattatga accgacgacg cggaaaaccg gcccgaaatc tcctggtctt
      301 atgcctgctc agcctggttt gggttgcgga cagttcaggc gcctcggatc ggcggctcga
      361 tgcgagcgat attaccaaac cggacttctt tcccattctg ccctggagcc cgtatcacgg
      421 ctggcgtgcg ccgtttatca cgcaccggcc aagcgggctg gaaagcattg cggactgcca
      481 gttcaacctg gcgggctttg tttgggcgca ggacttgcgc cgctgccgga agctcggctt
      541 gggggccatt ctcgtgccgg ccgcggcgga ggtcacctcg ccgggttatt accgcgaatg
      601 gaaaaaactt tcggatgcgg agattgaggg ccgcgttctg cgcatggtcc gcgcggcggg
      661 gcggaacccc gccgtcaagg gcttctacat catggacgag ccggggatca tggattttcc
      721 ggccctgggc aaggcggtgg cggcggtaaa gaagcacgcc ccgggcaagc tggcctacat
      781 caacctgttt cccaattacg cgacgcttgg cgcgccgaac atgtcccagt tgggcacctc
      841 caactacacg gaatacctgg agcggtttgt ggcggaggtt cggccgcagg tgatcagcta
      901 tgataactac atggtgcagt attccgagga cttgcgcgag ccgaagaagg cggccagcta
      961 ttaccgcaac ctgctcgaag tccggcgcgt ggcgcgggcg caccagcttc cttatttgaa
     1021 catcgtgagc gccaaccaaa ttcggccgca tacgcccatt ccctcgcccg ccaacctcca
     1081 gttccaggcc tatacgacgc tggcggccgg gtatcggggg atcacgtggt ttaattatta
     1141 tggcccgggg tataagtaca cgccgattga tcccgcgggc cgcaagaccc tgacgtgggt
     1201 ttatttgcgg gacatcaacg cgcaagtggc cgcgctggcg ccggtgatga gccggttaac
     1261 ttcgaccggc gtctttttca gtgcgcccgc gccggtggac ggcctgccgc tgctgccggg
     1321 caagttggtg gagtcggtcg cctgcgatgc gccggtgatg gtgggtgagt ttcggcacgc
     1381 cgacggggag gcgtatgtca tggtcgtcaa cctgagcctc gaacgttccg cccggttcac
     1441 gtttcaggcc gcctcaccgc gggaaaccgt ccaggtcatc tccgccgggg acggctcccg
     1501 ctcggattac gacctgaaga acgggttgtg gctgaccgcc ggccaggggg cgttgctggc
     1561 tttagggcgc tgacgaatcg ggcccgccgt ccccacccgg agggagggcg gcttccgggc
     1621 cgtgccggcg gcgcgggcgg gaccggccgg ccggggcagc ggatttcggc gcgggcggaa
     1681 agcgcaattc cgccgccttg agaacgctca cccggccgtg ggcggcgagg ttggcccaaa
     1741 cccagttgta gtacgcgcgc acggtgcggg cgttgaggag gggattgtcg gtcgtggtgt
     1801 gcgcatcggc gacgacgatg acgtggaaat cccggctcag cgcgttgcga agcgtggcgt
     1861 ccacgcagaa gtcggtggcg aagcccgtca gcagcagggt ggtgacgccg cgggagcgga
     1921 gcgccgcttc gagcgcggtg cgatagaacg catcgcacgt ggttttgcgg aggattaatt
     1981 cgccggggcg gacctggagg tgcgggtgca attcccaacc cgcgcggaag ggggccaggt
     2041 ccggttcgga ggggtcgccg tcgtgctgaa tgaagataac cgggacgccg gcccggcggg
     2101 cctgcgcggt gaccgcgttg atgcggccaa gcacggaggc ggcggcgaaa ggcggaggct
     2161 cggagccgaa cagcccgcgc tgcacatcaa tcaccaggac cgccgtgcgg gcgggcacgc
     2221 tcggcttcat ggctgcgggg ttacggccgc cgttcgcgga tggcggccag gatgccggcc
     2281 accacctcgg ctttgggttg ggcgccgagg ctgccccggc cgtggacgcg gacgctgacg
     2341 gcattggcgg ccaggtcgcg cgggccgacg accagcatgg tgtggacttt ggcctgttcg
     2401 gcctgctgga ttttgccgtt gatcttgtcg ttgctgtaat ccccctcggc gcgcacgagg
     2461 tgggcgcgca attcccgcac gagggcctgg gcgtagttca ccagcggggc ttcctcgccg
     2521 atggtcagga cgcgcacctg ctcgggggcg agccagagcg ggaagttgcc ggcgtaatgc
     2581 tcgatgagga acccgatgaa gcgctcatgc gtgccgagcg gcgcgcggtg gatgcacagc
     2641 ggggtcttct cggtgttgtc ccggtcgcga tagaccaggc cgaagcgctg gggcacggcg
     2701 aagtccacct ggttggtggc cagggtgaac tcacggccaa tgacgctcca gacctggacg
     2761 tcaattttgg ggccgtagaa ggcggcttcg ttggccacct cgacgtagtt gatgccggag
     2821 tttttgagca cgtcgcgcac catgttctcg gtttgaagcc acagttccgg ttcgttgacg
     2881 tatttgcggc ccaggccttc ccggccgtgg gtgctgaagc gcatgaggta tttctcaatg
     2941 ccgaagattt tgaagtattt gaggtacatc tcgttgacgg cattgaattc ctgcgcgaac
     3001 tgctcggggg tgcagtagat atgcgcgtcg ttcatgttca tcgagcgcac gcgcatgagg
     3061 ccgaacagtt ccccggactg ctcgtagcgg tagcagcagc cgtattcggc caggcgcagc
     3121 ggcaggtcgc ggtagctgcg cggcaccgcc gcaaagatgc ggtggtgatg cgggcagttc
     3181 atggccttga ggtagtagcg ggtgcccagc gcgcgaagct gttccgtcag ggtgtggatt
     3241 ttctggtcat attcccggtg ggcggccgac ccctccgggc ccgggggagg ctccccgttc
     3301 ccgccggggg cggcttgctt gagcgcttcc agttgcgctt cgaggtcgcg ctttcgggag
     3361 tgctcctctt cttcgagcaa ttccatgggc gggaacatgc tttcggcata gtagggcagg
     3421 tggccggagg tgagatacat tttctctttg gcaatgtgcg gcgttttggc gcgcacgtag
     3481 ccggccagga actcggtttc cttggccagt ttctccagtt cctcgaggat ggccgtgcct
     3541 ttgggcagcc agagcggcag gccggggccg gtgtattcgg tgtcaatggt ataaaggccc
     3601 atctcggcgc cgagcttgcg gtggtcgcgg cgcctggctt cctccagcat ttgaaaatac
     3661 gcgtcgagct gggccttggt tttgaaggcg gtgccgtaga tgcgctggag ctgggggttt
     3721 ttctcgtcgc cctggtagta ggcgctggcg acggtggtga gcttgaaggc gccgatattg
     3781 ccggtgcgca tgacgtgcgg gccggcgcac aggtctatga aatcgccggt tttgtagtag
     3841 ctgatgggtt cgtgggcggg gatttcggcc aggttgccga gcttgaactt gctggggttg
     3901 cccgggcgct cgctcaggcc gcccagccgg ccgctctcgg agtccttgat ggcctgctcg
     3961 cgggtgacga cgatcttctc gaagcgatgg ttggctttga tctctttctt catttcggct
     4021 tcgatggcgg ggaagtcctc cggggtgatg cgatgcggca ggtccacgtc gtaatagaag
     4081 ccattttcga ccgccggccc cgcggcgaac tgggcttccg gccacaggcg cagaatggcg
     4141 gcggccatga catgggcgca ggaatggcgc agccgttcca ggtcggtcat ctggttgcgg
     4201 tcatcgagcg ttttgcgttc gtcttgcatg gttcttattt aagcacaaag ggataaaaag
     4261 ggacactgaa tcttttggtt gcggtggaag ggcgatgggc gttcgcgccg gcgggcggtg
     4321 ccgccgccgg ggaccggcgg cagtctccgg cggtgggggg ctacatttgc cgggcggcca
     4381 gcgccagctt gcggcggaag tcctcgcggt tgcgggcatc gtagaactcg gcggggttga
     4441 agtcgtcggt caggatgcgg ccatgcgcgg gagcgggatg caccacgccg gcatagagcg
     4501 actggacctc gcggaggata tagggatgaa cccgggacag gtcgggttcc cgccgaaagg
     4561 ccagttcggg ttggtccgac gcgacaaaga agatggcgcc gtcgccgctg gcgtgcgccc
     4621 gcacggaggg gaatacggcc tggagggtgt ggtggaggga ggtggaaaag aaatcgcggc
     4681 cttcgtccag gctgccaaag ctgttgataa ccagcgttcc accggggcgg agcacgcggc
     4741 ggatggcgga gaaggcttcc cgcgtgaaca ggtgcgtggg cgagctgtcg cccaggaagg
     4801 cgtcgagaat gacggcgtca tattggcggc ggcagcggtt caggaagtgg cggccatcgt
     4861 caagggtgag gtggatttgg ttggtttgca ggtcgaagaa gcgaacggcg accggcacca
     4921 ccgcggggtt gatttccacc acgtccacgc gcacgccccg gtgggccagt tccatgggca
     4981 cgatgccgac ccccaggccg atgcaaaggg cgtcagccag gcggggggta taaacgtccg
     5041 ccagcccggc gagcgcgcag gtgaacaggg agacgctgag tttgcgttcc gggtcgtagg
     5101 tgttctggac aaggttgtcg ttgagataca tgcggtaggg gtaagcgcgc gcgtccacca
     5161 cctggagctg gccgaagtgg gaattgccgc ggaacaactc gacgacgtgg gcgtaggccg
     5221 gcgttgagct cagggagcgt tgccccgccg ccgccgcgcc cagcgccagc agcaggagca
     5281 gggctgcggc gccccggcgc cgggtgaaaa ggaagtaccc cgcgctcacc agcatcaacc
     5341 ccagcgcggt gccggtcatg gtgcgcgagt tgggcaggag gggaatcagc aggtagccga
     5401 taagcagcgt gccggccagg ctgcccaggg tgctgatggc ggtgagacgg ccgacgttgc
     5461 cgcccacgcc ggccacggtg gaggtgatca cgcggacgag gtaggggccg gtcgtggcca
     5521 gcagcgccag cggaatgaaa aagagaaggg ccgaggccag cagcgagccg acgggaaggc
     5581 tgaagtcgag gcaccagtag gccagaggtt cgcagagatg cacggtgaga accaggtaaa
     5641 gggccgccgc gaggatggcc cagtacagcc gcgcgagctg cggggactgg tcggccagcc
     5701 ggccgccggc gtaatagccg caggccagcg cgacgagcgt gacggcaatc tgggccgtcc
     5761 agacaaagtg cgacaggcca atgtaaggcg agagcatttt ggcgccgaga atctcgatga
     5821 tcatgatggc ggcgcccgtg accgccgccg tgaagtagag gtagccgcgc aggggcgggg
     5881 gcagggtggc tgggggcgcc ggtgcgggac gttttttcac ggcgaaggtt ttacgcgagg
     5941 cggagggcag acgcaacggc gaatgcagac gcctggcgcc gtcatccgcc ttgtttgccg
     6001 ggctgcgttg aggcagatgc ccggcgggat gagggccgcc cgggaaaggg gaggccacgg
     6061 aatcgggcgc gagggaggga gtggttgggc agactggttt tccgccgggg agaggcgaaa
     6121 aatgaacggc aaagaccgga cgggaaaagg agatcccgac ggcaaaagcc gcaactgaaa
     6181 ccgggagccg ggcttattca gcggcttaaa aacgcggggt ccgggaaaaa gaaaccaaat
     6241 gcgcttgcta ttggcgccta tctccctcat agtagtcgca gactagagat aatttgattt
     6301 atggctgatg tagcaaacaa atacccagat aacgctccgg gcaagtatta cgtagataat
     6361 caatgcattg attgcgacct gtgtcgcgag acggctccgg ataactttat gcgcaatgaa
     6421 gacggcggtt attcgtacgt ttccaagcag cccgcgactc ccgaagagga ggagcgctgc
     6481 aaagaagcga aggaagcctg cccggtggaa gccattggcg acgacggggc ctagccgttt
     6541 tgctcgtttc atcccgcccg cggccgttcg ccgcgggctt tttgctaaag cgcgtcctcc
     6601 ggcgcggctc gagtggagga gttgcggtac cgttgtcgtt gccgtgcttc ggaagcgggg
     6661 cggtgttgtg ccgctgacga acgaggtttc caattccatt gtttgcgttt gacttgagtc
     6721 ggctcgtagc gataatcgct ctaccaaagt aaattatggc caagcttgtt gtccttagcg
     6781 cgggaatgac cggacgcaca cacgagttgc aggtggagag aaccaccatc ggccgggttg
     6841 aggataacac gttcaccatt gccgagcctt ccgtgtccag ccatcactgc gaggtattgc
     6901 tgcgcggcag cgatatcgtg gtgaaggacc ttaattccac caacggcact tacatcaacg
     6961 gcgagaaaat caccgaggcc atcatcaagc cgggtcaaat cctgcggttg ggacaggtgg
     7021 aagtgcgcct ggaaacggac gctcccgcgg ccccggccaa gaagaagatg gatcaaaccc
     7081 tggtcatgca gcgcggcgtc agcctcaacg acctggagca gagcgcgcac ggcgggggat
     7141 ttgatacgaa gggaacggga tttacgaaga agaccgacag gggcagcaag gttttctgga
     7201 tcgtggcggc cctggtcggg ttgattatcg tggtggggct tctctacgcg ctgaccacga
     7261 tatccggcaa atagatcagc cttccttcag ccagcgggct gcatccacgg cggcgtaggt
     7321 aataatgatg tccgcgccgg cgcggcgcag ggcgaggagg ctttccagca ccaccgcgcg
     7381 ctcatcaatc cagccctggg cggccgccgc cttgaccatc gagtattcgg cgctgactgc
     7441 gtacgcggcg gtgggatagc caaaggccgt ttttacccga taaacaatgt cgaggcagga
     7501 cagcgcgggc ttgaccatga cgaggtcggc gccttcctga atatccagtg ccacctcgcg
     7561 cagcgcttcc gacgcgttgg ccgggtccat ctggtagctg cgccggtcgc caaacttcgg
     7621 cgcggattcc gccgcttcgc ggaagggccc gtagaaggcg gaggcgaact tggcggcgta
     7681 ggccatgatg ggcgtatccg gaaagccgtg ccggtccagt tccgcgcgga gggcgcgcac
     7741 gcggccgtcc atcatatcgc tgggcgcgac catgtccgcg ccggcttccg cgtggcttac
     7801 cgccgtgcgg gccagcaatt tcagggtggg atcgttgagg atgcgggcgc cggatttgtg
     7861 gcgctggacc aggccgcaat ggccgtgtgc catatattca cacaggcaaa catcagtaat
     7921 caccagcaat tcgggcaact cccgcttgag caaccgcacc gcctgttgca cgatcccgcg
     7981 ggcggcatac gcgcccgagg cccgggcgtc ctttcgttcc gggatgccga acagcaggac
     8041 tgccgggatg ccggcttcga gcgcccggcc ggcttcgtgc aataattcat cgggcgatag
     8101 ctgatacacg ccgggcatgg ccgcaatcgg gcggcgcagc ttgcgtccgc tgcgcacgaa
     8161 taacggcaaa accaattgcc cggcgttcaa cgaggtttcc cgcaccaact gccgcagccg
     8221 gggtgattgg cgcagtcgcc gggggcgatg cgcggggaag gagcccgctg gatagaaact
     8281 catgcccgga ttctaaaaac gcggcgcaaa gtgcaaagcc taaactgcat caccgggcgg
     8341 cgggttttgc ccgcctccac cgccgggccg atggtggctc cgaaagaccc cggaataggc
     8401 gcttgccaag aggggcttta tttcgcacaa taccatatta tgaagaatct aattggcgtc
     8461 atcgccttta ttttggttct ggtgtgcctg gggctgggga ttgggctgat cacgatcaag
     8521 aagcaggcga cggatcaaca gcatgatctg actgagaaga atattgcgtt gtcaaaccgc
     8581 ctggtgcagg ccaatgacga actggatcaa cagaagcaaa ccaatgccct gctggaaaac
     8641 gaccgcgagg agcggaagaa ggcattcgag gcgctgacaa ataactacac ccaggcactg
     8701 atctccctcg cccagaccgc caataccctt gctcaaaccg aggccgccct caagagcagc
     8761 caggaagaaa ccgcccggcg cgatgcgaag attgcggagt tggaggagca gaaccaggcg
     8821 ctcgacaagc gggcggcgga tttgaacacg gcgatcaccg atttgacccg ccaaatcgag
     8881 gagacgaagc ggaagctggc ctcctccgaa ggcgaaaagg atttccttaa caaggagctt
     8941 aagcggctca tggcggaaaa ggccgaactg gagcggcagt tcaacgattt ggccattctg
     9001 cgcgcgcagg tttccaagct gaaagaggag ctttccgtcg cccgccggct cgcctggatt
     9061 cgccagggtc tcttcgccag tccggatcaa aagggcgcgc aaagactgat gcggagcccc
     9121 tccgcaacgc agcccaaagc ccctccgccc aattacgatc tcaatgtgga ggtcaccgcc
     9181 gacggttcgg tcaaggttat cccgcctttg accaatcgtc ccccggcgac caatccaccg
     9241 gcccactagc aacgccgggc gggctgtgaa tccgcggggg ctagtgcctg ccgccgactg
     9301 gactatttac ccacgactat gccgacttac gaatacgttt gcgagaaatg cggacatcgt
     9361 tttgagaagt ttcagcccat taccgctccg cctttgacgg cctgccccaa agacctgtgc
     9421 gcccgtcaac cctggggcaa aggcaaggtt aaacggcaga tcggcgcggg cgcgggcctg
     9481 attttcaaag gcaacggttt ttacattacc gactaccgca gcgagaaata taaggaagcc
     9541 gccaaaaaag acgtcccccc cgccgctgcc tccagcagca cggagaagcc cgcgaccagc
     9601 tccggcggca agcccgccgc caagcctgcg ggcaaatcgg cctccaaggc cgccgccagg
     9661 aattaagcgc cctgattccc cgcgccaatg gccgggccgg gtctttccgt gatcgggtaa
     9721 aaataccagc gccggcagcg ggaggcggat gcgaacaaca ggcgatccgg aatgaagatt
     9781 caggtcaaag cagtcaccgg gttgggaatg gccctggcaa tggcctcggc cctctggctg
     9841 ccggccgggc cctgctgggc cggagctacg cagttgccgg cgaacctggt atcggcgcgg
     9901 agccaatccg ggcaattcgt ggtctatgcc gggtccaccc cgggctcgat tccgcctgtc
     9961 ctcgaacctg cgaagaacca gggtttcatt cggttggaag ccaccctggt ggcggtatca
    10021 tgcgagcgca tcaaacagct tctcacgcgt gaactggacg accccgcgcc gtggcgcggc
    10081 acgatttacc tggtgctcta tcccgcccgc ggggccagcg acccgattac gattaccacg
    10141 gagcggttca aggacgagtc gcattaccgg gtggatatgc cggacgtggt ggagcggatt
    10201 cgctacgtgc gggccgtggt gcaggtcttg ttaatcgaga tggcgaatcg cgcaggctgg
    10261 gctcaggccg cggaggttcc cgcctggctg agcgaagggt tcacgcagtt gctgctggcg
    10321 gccaatgaag tcgaaatcat cctgcccgcg ccgtcggaga gcacgagcgg ccttaatctc
    10381 agcaccatga cggtccgaag ccgcaaagaa tccctggagg accaggtccg gaagaagctg
    10441 cgcgggcgcg cgccgctaac ctttgaggac ttgagctggc ctacggagga gcagcggtcc
    10501 ggcgaggctg gcgaggttta ctccggcagc gcgcatttgt ttgtgggcga actgctgcgc
    10561 ctgccggaag gccgcgccgc gttgcgcgga atgctgacgc ggttgccgca gcactacaac
    10621 tggcagtttg cttttcttaa ggcgtttagc ccccgctttg aacgtccgct cgatgtcgaa
    10681 aagtggtggg ctttgtcccg cgcgcagatc ctcggacgcg agccggcgac cgcctggtcg
    10741 ctggaggaaa gctggcggaa gctggaccag gcgttgcgcg cggccgtgct ggtgcgcgct
    10801 gcgaccaacg actctccgct gcgcgccgac gtgccgctgc aaacggtgct gcgcgaatgg
    10861 gaaccccaac ggcaaatcct ggcgttcgac cacgcgctgc gccaactgga gttgctccgt
    10921 ccgcggctgg ctccggagct ggccggactt gtggaggact acagccaggc catcaaggct
    10981 tacacgcagc agcagggccg cggcgggacg gtgcttcccg ccagccggaa agccagccag
    11041 cagcgggctg tcgaagccgc gatccagcaa ctcgacgcgc tggatgtccg ccgccaggcc
    11101 atgctctctc cgtcattggc accggaggcg gttccgcttc ccgccgcttc cgccccgccg
    11161 ccttgatgtt gcacccggct gagttggggc gggagttgcg ccgcgacgac ttttgcgcca
    11221 gactcgggcg gtgaaaccac gcgttcgctt tgcgcctagt ccgacggggt tcctgcatat
    11281 aggcggggct cgcacggcgt tgtttaattg gctttatgcc cggcatgcgg gcgggacttt
    11341 catcctgcgc atcgaagaca ccgatgcggc acgcaacacc caggcggctg tcgaggttat
    11401 cctcaacggc ttgcgctggc tggggctgga ctgggacgaa ggcccgctga gcggcgatcc
    11461 cgccgggccc agccgcggcg actacggtcc ttactttcaa agccgccgcc gggacattta
    11521 ccagcggcgg atcgaggccc tgctcgcgaa agggctggcg tatgaagcgg acggaacgat
    11581 caagttcaag atggaccgtg cccccatcct cattccggac ctggtcgtcg gcgatgtgcg
    11641 acgcgagttg accgaccgcg agcaagcgga cccggatttc atcatcgtgc gctcggacgg
    11701 gcaaccggtg tttcatttcg tcaacgtggt ggacgacctc gaaatgggca ttacgcatgt
    11761 catccgcggc gaagaccacc tcagcaacac cgccaagcac atcgccctgt tccgcgcgct
    11821 ggatgccgac ccgccccaat acgcccacat cccgctcatc ctgaatagtg atggcaccaa
    11881 gatgagcaag cgcgaccagg gcgcctcgct catgacctac ggcgaggaag gctacctgcc
    11941 cgaagcgctg gtgaactacc tgtgcctgct tggctggtcg cccaagggca atcgtgaaaa
    12001 aatgccgctt acggaagtga ttgcagcgtt cgacctgccg caaatccttc gccacaatgc
    12061 gcggttcgat ctcgccaagc tggtttggct gaacggcgaa tacctgcgcg agttgagcga
    12121 cgaacgcttc tacgagttat ccgccgaagc gctgcggcgc ggcgggttgg agcttgcccg
    12181 gtatccggac gcctacgtgc gtgccgcgct ggaaacatgc aagggcaagg tgaagctgtt
    12241 cagcgaattg ccggcctacg ccgggttcta cttcacggac gaaataaaat acgatcctgc
    12301 ggcggcggaa aaggagttca ccccggaaaa ccggccgcgg gtggacaaac tgcgcgcggc
    12361 actggccaaa ctggagccgt tcgagccgga tccaattggc gcgacgctca aagccgtggc
    12421 gcaggaactg ggcgtgaaaa cgggtgtgct ggtgcatcct acgcgcctgg cctgctgcgg
    12481 caacaccgct ggccccagtc tctatcattt gctggcaatc ctcggcaaag accgcgcgtt
    12541 ggcgcgcctg gatcaggcgc tcgcccggat ggtctaggcc accttcctta accggccagg
    12601 ttgcggttct tggcgcgcga atcagttgcc agtcgcgcct tgaaggccag cagtttcttc
    12661 tggagcgccg catctccgac tgccaggatt tgcagcgcga gcaagccggc attgcgggcc
    12721 ccgccaatcg cgaccgtcgc caccggtatg ccgctgggca tttgtacgat ggacaacagc
    12781 gagtcgagcc ccttcagtgc tttgctctcg attggcaccc cgattaccgg cagcgtggta
    12841 aacgccgccg ctacccccgg caaatgcgcc gcccctccgg ctccggcgat gattacctgc
    12901 aaaccgcgag cccgcgccgt cgtggcataa cgctccaggt cccgtggcgt gcgatgcgcg
    12961 gagatgaccc gctcttcgct ggccacgcca aattcagcgc aggcctccgc cgccgccttc
    13021 atggtgggcc agtcggagtc gctgcccatg atgattccga tccggggttt gggagtttgc
    13081 ttcatgggcg gtatgattta ctttggtttg tggaaactca atcctgaaat ggccggttgt
    13141 cgccgaattg taaggctggc tttgttaggg ctgaaactat ggcgattacc ccgagtgcgt
    13201 tgctcaccgg gtgcggcgtc cgccgatgga gttgacgaag cgccctgatg caggcacagt
    13261 caattcaggc ttggccatga caaccgccaa caaaatcacc atcctgcgca tccttctgat
    13321 tcctgttttt gtcgtggcgg tgctctacta tgggcagggg ggcaagagtg tgcactggtg
    13381 gttagcggtg ttgtgcttcg ctaccgcgtc catttgcgac ggtgtggatg gctatatcgc
    13441 gcgccgcttc aatcagcgca gcgaactggg cgccatgctc gacccgctgg ccgacaagct
    13501 cttgctggcc tccgccgtga ttgtgctgag ctttgatcac cggccctact tggaagcagt
    13561 cccgctctgg ctcaccggca ccattattgg ccgggacatg ttgctgttaa tcggtttgct
    13621 ggtcattcgg ctaatggtcg ggaaattcac tatccggcct cgggtggtgg gcaaggtcgc
    13681 cacggtgttg cagatggccg tcgtgctgtg gatcctcttg cgatggccgc aacgctggct
    13741 cttgttctgg accatcagtg ccgctgtctg caccggtgct tcagggttgt tatatgtttg
    13801 ggacggcagc cgccagttga gcgcccaccc gtcggccagt cccaagcccg ggagctaagc
    13861 cttttgcagt tggggctatc ttgttgccct cgggcggcca ggtgattgac ctttgccggg
    13921 attcccgcca ccttcccggc atgctgaata ccggaccgct gaaacaattt ctcgaagacc
    13981 aaatgccggc cgctctgggt attttgcggc gcatggtaga gattaatagt tttacgggga
    14041 atcgtgttgg cgtgaaccag cttggccgat ttacggccca atgttttgcg cccttggggt
    14101 tcagcgcgga gtatgtaccc tcggccaacc cggcttgggg cgatcatttg gtgctgacgc
    14161 ggcgcggttg ttcgagcaag agcctcgctt tgatttcgca cctcgatacc gtattcccgc
    14221 ccgaggaaga ggaacgtaat aactttcgct ggcaactgga aggcgaccgc atttttggtc
    14281 caggcacaca cgatatcaaa ggcggctcag tgatgatgtg gctcgtgctg tcggcattac
    14341 aggttcagtt cccgaagtta tttgcgaaca ttacctggaa gctgatttgg aactcttctg
    14401 aagaatgcta ttcgcctgat tttgcggaag tctgccgcac gcacttcgat gacggcacgc
    14461 tggcggcgct ggtctttgaa tccgaagggc gcctggataa tatcccgcag ttgatcgtgg
    14521 ctcgcaaagg gcgggggacc tggcgggcga cggtcggtgg tcgcggctcg cacgctggcg
    14581 gcaagcacgc gcacggtgtc aacgccatcg ttcaactcgg ccgcacggtg caacgcatcg
    14641 ccgccctgac gaattatgag cgtgggctca cggtcaatgt tggcaccatt aacggcggaa
    14701 ccgtgctgaa ccgtgttccg cacgaggccg tggcggaggg ggaatttcgc gcattcacgc
    14761 cggaagtctt tatgcaagcc aagcaggatc tgcttgcgct ggcggggccg ggcgaggtgc
    14821 acagttccgc cgatggttat gcctgcaagg tccaaattga aatccttacc gaatccgctc
    14881 cctggccaag gaatccaggc accgacggac tctttcagct ttggaaacag accgccgacg
    14941 aacttgggtt ggcgttaaac tgcgaagagc ggggcggcct gagcgacggc aacctcattt
    15001 gggatgtggt gcccacactc gacggcctcg gcccctgggg cgacaacgac cattgttccg
    15061 agcgcagccc cgacggctcc aagctgcccg aatttgtgga agcgagctct tttgtcccca
    15121 aggccgtgct gaacattgcc gccctccaga agttgtgcgg atcctgaggc tggtgcccgg
    15181 ccgtgctcat tcgggcgata tcgtgtctaa catcgcaact ctcgcgcggg gccttgtctc
    15241 ctttcccata aagcatgaaa tagaggcgtc aaaggttgac agttctatta tatttaagag
    15301 gtaactgagc aatatgactg gcccgcgttc cgtccgcaat cttgccctga ttggattcat
    15361 gggcagtggt aaatcttgcg tgggtcggat ggcggccgat ttgctccatt tcaccttttt
    15421 ggacactgac caagtcatcg aggcgcgagc cgggaagagg attagtgaca tttttgctca
    15481 ggacggcgag aggggctttc gtgagtggga gcgacggata gtcgaggaac tgacccggcg
    15541 tgagaagacg gtcattgcca ccgggggcgg attgccgatc gacccggaca acctggccag
    15601 ccttaagacg cattctctgg taatctgtct ttgggcctcg ccggagacca tctgggaacg
    15661 cacgcgcggg cacagccatc ggccgttgct gaacgagagt gatccattga caaagattcg
    15721 ccagcttttg gcagctcggg agccctatta tcgccaggcc gatgtgctgg tgaacaccga
    15781 aatgcggtcg ttacgcgagg tcgtgcagca cgtgctgcac cagttttatc tggtgcgatc
    15841 cggtcaccga tgaaagaggc tcttcggcag cgagcgcgtg agttgggttt cgatgactgc
    15901 cgcgtcacca ccgccagtcc tccggagact gctccgtatt tccagcagtg gctcgccgca
    15961 ggacggcaag gtcggatggc ttggctcgaa cgctctatgc ggaagcgaac agatccgcag
    16021 gtagtgctgc ccggggcccg aagcattatt acgctggcgg ccagttatca ctgtcgcggt
    16081 tcccaaaccg gttccgtcgc ccgctatgct cgctttgacg attatcacaa catcctgagc
    16141 gagcgattgg aaatactcac tgattttgtg agccaactag gggagagcgg cactcgctcg
    16201 caatggtacg tggatgtcgg cccggtgctg gagcgggatg tggctcagcg tgccgggctg
    16261 ggttttattg gtaagcacac caacctcatc agccgccggt taggcaactg gttttttctc
    16321 gctgagatta tcactaccct tgccttggag cctgatccgt cggagcgcac tcgctgtggt
    16381 tcctgccggc gttgcatcaa ggcctgccca accgcggcga taattgcccc ctttcaactc
    16441 gatgcacggt attgtatctc ctacttgacg attgaactga aagggagcat cccggtcgca
    16501 ctgcggccgg ctatcgggaa ccgcattttt ggctgcgatg agtgcctgct ggcgtgccca
    16561 tggaatcgct ttgctcggga agggcagatc atgaagacgt atgcgcgccc ggggtttacg
    16621 caattggatt tgctggagct gttgactttg ggagaatcgg agttcacgcg tcgttttgcg
    16681 ggcacgccca tccagcgcgt gcggcgtcgc ggcctggtgc gcaacgtctg cgttgcttta
    16741 ggaaacaccg gtggcgagcc agctttggcg gcactgcata aggcaactag tgatgcagaa
    16801 ccgctggtag cgcagcatgc tcagtgggcg gtagagcaag tgaccgcacg gattagcacc
    16861 catcgcggag ttcttccgga ttgaaacaga tttgcataga cacagcgcca gttgtcttta
    16921 ctccggcgta tcaagtttgg gaatggacaa cctcctcgaa aagttagatg cggaaggtcc
    16981 accattcggt gtgagagaga gtagggtaaa tccgttattt caatctccat catcgcgccg
    17041 aaattgattt aggcatctaa agcttgaact tgcggttaaa aatcgctagg agtggagcaa
    17101 taaatgacaa cctgcacgca tttcttatga agaaactcaa tgtcggactt atcggttgtg
    17161 gtttcatggg ccgcacgcac tcgaatgcgg cgcgccgggt gaacaacttt tttgaccttg
    17221 aataccagcc tgtgctcaaa gccgtttgtg atttggatgc ggcccgggcc caggcgtttg
    17281 ccgcccagtg gggttacgaa tcctgtgaaa cggactggcg caagctcatt gcccgtcagg
    17341 acatagatat ggtggacatt accgtgccga acgacttgca ccgggaagtg gcggtggcgg
    17401 cggctaaggc cggcaaggtg attctgtgcg aaaaaccgct ggcgcgcacc ggcccggaag
    17461 ctttgaaaat ggtgcaggcg gtcgagcggg cgggcgtccc caactttgtg tcctacaatt
    17521 accggcgtat tccagcagtc accctggcca agcaactcat agacgagggc aggcttggac
    17581 ggatttttca ttaccgcgcg aagtttttgc aggactggac gatttccaaa gacctgcctc
    17641 agggcggtcc ggcattgtgg cggttggatg cgaaggtcgc tggcagtggt gtgaccggtg
    17701 acctgctggc ccactgcatt gacactgcgc tctggctcaa cggtgattta gaacaggtaa
    17761 atgccatggc ggaaaccttt atcaagcagc gtaagcatac agtttccggc aaggtcgaga
    17821 aggtggggat agatgatgcg tgcgcttttc tggcgcgatt taagaatgga tcactggcta
    17881 catttgaatc cacccgtttc gcccgcgggc acaaggcgct ttacaccttt gaaattaacg
    17941 gcgagcacgc ctccctcttc tgggatctgc atgatttgca tcgcttgcag tactttgatc
    18001 accgcgacgc gggtcgtgtc cggggttggc gtagcatcca cgtcactgac ggggaccatc
    18061 cgtacatggg aaagtggtgg gtgccggggc tgcagattgg ctacgagcac agctttgttc
    18121 accagcttgc cgattttctc cagggcttgg ccacgggaaa atctgcacgt ccgaccttcc
    18181 gcgacgcact ccaaacacag tacgtctgcg atgccgtgct caagtctgca aagacccgcc
    18241 gttgggaaaa ggtgccgaag gttaagtagc taaatattcc ctgcccttta taaaggcggc
    18301 aggtcaccgt gccggcactc tgccagttga acgtttcaac acgtaaacgg ccaactttcc
    18361 ggaagcaggg cctgggtgcg ttccatcgtg tccttgccta tgaatcagcc ttcaactcac
    18421 gctgacgccc atcaaaaact cggcattact gtgcgtcttt ttgagtttgt ttaacaccag
    18481 ttccatcgcc tcgaccggtg gcactccagt gagtgcccgg cgcaggatga ccactcgatt
    18541 gtattcgtcc ggatgataaa gcagttcttc tttgcgcgtg ccggaacgct cgatattgat
    18601 ggaggggaaa atgcgccgat ccaccagtgc gcggtccagg caaatctcca tattgccggt
    18661 ccctttgaac tcttcaaaaa tcacctcatc catccgtgac cctgtatcta ccagtgccgt
    18721 cgcgatgatc gtcagggacc ccccttcttc gatattgcgg gccgctccga aaaaacgttt
    18781 gggtttgtgc agggcgttgg catccacacc gccggatagg atcttacccg aatgcggctg
    18841 cacggtgtta taggcacgcg ccaggcgcgt aatcgagtcc aacaggataa ccacgtcgcg
    18901 cttgtgctcg atcattcgcc gcgctttctc gatcaccatt tccgctactt gtacgtgccg
    18961 ctccggcggt tcgtcaaagg tcgaactgat gacctctgcc cctcggcaag ttcgttccat
    19021 gtctgttacc tcttccgggc gctcgtcgat cagcaggatg aacaggtagg tctccggatt
    19081 attcttcaga atggcgttag ccaccttttg catcagcacc gttttgcctg tgcgtggcgg
    19141 ggcaacgatc aatccacggc tgcctttgcc gatcgggcaa accagatcca gcactcgcgt
    19201 ggaaagctcg tctggagtgg tttccagaat aaagcgctgg ttgggaaaaa gcggcgtcag
    19261 gttgtcgaaa agaatcttgt cttttgcctt gtccggttct tcctggtcca ccgcctcgac
    19321 tttcagtagc gcaaagaact tctccttttc tttcggtgga cgaatttggc ctgctaccag
    19381 gtttccagtt tgcaggtcga agcgacgaat ttgcgacggc gaaacataga tatcttctgg
    19441 acagggcaga taattaaaac tttgcgaacg gagaaaccca aagccttcag gcaacacttc
    19501 caataccccc tctgaaaaca gcactccggc ccgctccgcg tttttttgca ggatatgaaa
    19561 aataacctcg tgcttgcgca ttgttccgaa gttctccacc tcaaggtccc gcgccatttg
    19621 gttcagatcc ggcatggaca tcgcttgcaa tttggcaatg ttgaccgaag ccggggcccc
    19681 gggctccttt tccctctccg gggcgtgttt tgtccgtggc ggttcctcta ctagtggttc
    19741 cactggcgtg gagggcggca ccaatgcttc cgcgggtgcc tctgccgtag tgtctagtgg
    19801 aacgcttact cccgcgtcat tgccgtttaa gggcgcagca acaacttctt tggccgcttt
    19861 gcttttggcc tttttttttg gtcttggcat aagtcagtac gtttagcctg taaaatgtat
    19921 ttcgggtaag accgctatga aactgtctct cagtccagcc ctttgactgg gcaactaccg
    19981 cttcgattaa tttaatatgg aatggccgcc taccaggagc atcgtgttaa ctttccaatt
    20041 attccgcggt gcggcaaggg aaatctcatc aaattcagcg gcagcctgac gtattcttct
    20101 tgggctgtca agtcttagct aatgtcatag tgcgctgaag aattggtccg ccgttcaata
    20161 gggcaacgga aaccgcgcgg tcaactcgtg tacccgctgc cgcacctttt cgctgacttc
    20221 cacattcttg aggtctagca acacctcgct gatcatgtcg gcaatggccg acatctcgga
    20281 ttctgccatg ccgcgcgttg tgactgccgg tgtgcccagt cggatgccgc ttgccaggaa
    20341 gggggaccgt gtctcgaatg gaatcgtatt cttgtttacg gtgatgcccg ccagatctag
    20401 agccgcttgg cattccttgc ccgtcgctcc gcgtgcgcct acgttcacca gcatcaagtg
    20461 gttatccgtg cctccgctta ccaatcggaa attgttctgc ttcattcccg cggccagggc
    20521 tgccgcgttc ttgacgattt gcttctggta ctcctggaac gacggctgca tcgcttccag
    20581 cagacacacc gccttggcgg caatcacatg cattaacggc ccgccttgga tgcccgggaa
    20641 ggtctgtgaa tctatctcct ttgcgtattt ttcccggcac aacaccaacc ctccgcgtgg
    20701 accgcgcaac gttttatggg tcgtggtcgt cacaaaatcg gcgtagggta ccgggtttgg
    20761 gtgcaaccct gcggccacca gcccagcgat atgtgcgata tcggccaaca ggtacgctcc
    20821 gacctcgcgc gcgatttgcc ccaaccgctc gaactcaatt atccgcggat aggcactagc
    20881 tcccaccgtg atcatcttag gccggtgttc ccgcgccatc tgggctagtt gctcataatc
    20941 aatgcgttca tcatccttcc gcactccgta atggatcacc tcgtaaaacc ggcccgaaaa
    21001 atttgccttg ttgccatgcg tcaaatgccc accatgcgtc aggtccattg tgagcagttt
    21061 gtctccgggt ttgagaaagg caaaatacac cgccatgttt gcctggctcc cggagtgggg
    21121 ctgtacattg gcgtgctcgg ctccaaaaag tttccgggcg cgctcgatcg ccaattgctc
    21181 aattacatcc acattctcgc agccgccata ccatcgcttc cttggatagc cctcggcata
    21241 tttattggtc agcacggatc cctgtgcttc cattacggcg ggactggtga aattctcact
    21301 cgcaattagc tcaatgtttt cttgttggcg ccggacttct tgcttaatgg ctgcggccac
    21361 ttccgggtca actgcttgca atctgtaatc agcgggcata atgcgttttt ccataaaggt
    21421 tcctttcttt tggtgtccct cgaaatccga ggtgcggggc tcggtcgccc cggattcaat
    21481 gaatctcagc aatgatgtga tccctcgttt gatttggtcg cggcaatcta catatatcgc
    21541 atatgagcca ccgatcggat cgcgaatatc tatttcaccc ggccccagtt gttcatcgaa
    21601 ctcgcgcagc agaaaggtct tttcccgtgc tggtgggtag agggtggcta tcgtttcaac
    21661 gtgaccgaga gtcatgccga agatgtaatc agcctggctg actagttccg cggttagcgc
    21721 ctggctgcgc agacccgaaa tgtctatatc cagttctttg agggcagcga ccgcatgcgg
    21781 agtaggaggc tgcccatctg ccgcgccgag acctgccgac accgcgcgat aatctccccg
    21841 ttcccggata aactgccgga agatgccttc cgccatcgga ctccggcaca cgtttccggt
    21901 gcaaacaaac aggattgttt tcattcaatc atgcactggc aatttcagga gggccaatcg
    21961 cgtaaccatt tatcaagcga ttaagatcga atcatcggcc gaaccaacgc taagccccgc
    22021 agtaaatcca gcgcccgagc taacaccgga tcccggacaa tcggttgctc cggttcgctt
    22081 tcccgtgggg cgttcaggct tgcctcgctt aacccctcgt gccgctcgcg catcagttcc
    22141 gcctcattca agcgcggtcg ccgcgttgct ggggtcgctc ctgcaatacc ggttccaact
    22201 ggcagattcg tcttcgctaa agtttgaaaa gcatcggcaa aatacttttg ttcctcagcc
    22261 ggattgacag ctaccacaat atccggagtg agcccttctg tggtcagcgc cgagccatcg
    22321 cctaactgga tcatcgccgt cgcaattcgc aaacgaacgc catttttcaa aggaaactcc
    22381 tgtgtcacct gtgcctggcc tgccgttttt cctcccagga gcaaggcggt tccggtcttc
    22441 cgcagcaccg ctgccatcgc ttctgcggcc tccgttgttt gccgattcac gagtactgcc
    22501 acaggcacgc agatcgcgtt gctctttgtc tgcgcgcgca ctattccgtt gccacaatct
    22561 aacaatggtt gctccttttt cacgaataaa tccgctgccg cagcggccgc tgcgtaatca
    22621 tcaccccctg catagcgcaa atccagtata agtccgttaa ccccgtttgc agatcccgga
    22681 ttactccatg ttcgagcaat tgcttccgat aaaccctcac ctacctgcgc gacccgtata
    22741 tacaggatgt ctccttgaaa cacttccgct ttgctcaccc gaggtcctgc catcgtccga
    22801 ggagccttcc cattggtaat aaggaccact cgcgggctga gttccgccac cagtccccgg
    22861 acaacggcct cgttcaattg cgtctcggtt atcccgacca gatgggagcg gatcaagtca
    22921 tatacttctt tgcagtctgg cccggaggtc tcctcagccg tggctaccgt cagcagtaag
    22981 cccgccaacc cgccacatag caccagccag accgtcacga gacaactggt tccaattcgt
    23041 atcatgtatt gctataatgt ccccttcagg cttcaaagta aagctaacgg tctaccatgg
    23101 cgtgagcagg cgagctttaa ttggaggcaa tttgatgtgt cggccgtccc gcgatccgtt
    23161 gcaactataa cttgtctggg ctgcttaaca agtgcgctac gcgagccaga aggcggcccg
    23221 ccgcacctcc gtccaggacg cggtgatcga agcttaaagt gagatttgct tccatagccg
    23281 gcacgaactg ggctttcgac gtgtcccaag caggcactgg gcgagccgcg cccattccca
    23341 ggaccagcgt ctgctccggc agcgggatag gcgtcgccca agtcagacca aaggtgccaa
    23401 agttggtcac ggtggcaata gagccgccag tgacgtcggt tggcagcttg cgttcatgag
    23461 ccagtttgat caactcatcg tagcgtttca ccaaatcctt gagcggcgtt ttttctgcgc
    23521 tgcgaatcac tggaaccagc accccttctt cggcttcgac agcgaacccg atatcaatgg
    23581 ccgtagggtg aacaatctta ttgccgatga gtcggccggc gggagtgctg ttttcagcca
    23641 gtgccaaggc cagcgcccgc aaggcgtaaa gggccgggcc gggcttggga tcacacatct
    23701 tgcggtggtt cagaagcggg tcgaaacaga cgggcaaagc gaccgtagcc agcggccggg
    23761 tccaactgcg tcgcatggcg tcggcaacag ccacgcgcat gggcgaagcc tgcgtcagct
    23821 tatgtcgttc cagattggca ataaaccgtt caaagtcctg aatggtcacg cgcccagcgg
    23881 cgccactgcc ggggagaccc gccaagtcgg ccgcatggag tcccaattcc atcatacgcg
    23941 ccttcatgcg cggcgacata tagctggcac cttcagcatt agccggcacg ggcagaccgc
    24001 gaaccgtcgg ttgcacgcct ttatggctgg cacctgccag ttcccggcgt gaaagatcaa
    24061 actcggactc gtctccgcca tcgtcggcgg aggaatccaa atccaaaccc agccgtgtgg
    24121 cgtcttccgg gctggcttcc aaatagccta acaccgcgcc aaccgggtag ctggtgcctg
    24181 gctttgccag gaatctttcc atccgcccag ggcagggtga ggttatgttc atggtggcct
    24241 tgctggtttc caccttgatg atgtcttggt cggtttgcac gtggtctcca accgggacca
    24301 gcacgttgac gaccgtggct tcagcgatag attcgccgag ttgaggcatg atgattggga
    24361 tggagggcat ggcggctgaa aaacgtttac aatcgcaaca aacggcgggc agcggcggct
    24421 atgctgtgag ccgtgggacg atgcgccgcc cacaaactgg gatgataagg aaccggggtt
    24481 tctttcgcgt tgagccgttg cggcggggcg tcgagcagcc caaagccttc actggtcaca
    24541 cgtgcgatca cctccgccgt gacgcccccc cagggccagg cttctcccac gcagaggagc
    24601 cttccggtgc gtgctacgga ggccataacg gtatcagtat ccagaggttt cacggagcgg
    24661 agatcaacta cttcaatctc cgctccttcc gacgccagtt catccgctac tgcgagtgct
    24721 tcatgcacca tagcgctgta ggccaccaaa gtcatgtcgc ggccagctcg cgcaatgcgc
    24781 gctttgccgg ttggcatggc ctcgacgggc agttgcgcgg ctttcaggtg gtaatacaaa
    24841 tacttgtgtt cgcagaaaat aaccgggtca tccagcgcga tggcttctaa caacatgctg
    24901 taggcatctt cgaccgtggc cggagtcatc acgatcaaac ccggataatg cgcgtaaacc
    24961 gcctccatgc tctggctgtg aaagggcccg ctcccggaag tgccgccgca gggcaagcgc
    25021 accgtaatcg gacagggcac gcgcgttcgc caatatagcg tcgccgcctg attaactatc
    25081 tggttgaacc cgagcgtcga aaaatccgca aactgcattt caattatcgg ccgcattcct
    25141 tgaatggcgg ctcccacggc cagccccagc atggcatctt cactgattgg cgaatctatc
    25201 acccgccctg gaaagcgctg cgccaggttt ttcgtagcct taaaagcgcc accaaaggtg
    25261 ccgatatcct gcccataaat gaacactcgc ggatcatccg ccagcgcgcg cgcctgtgcc
    25321 tcgcgaattg catccaggta ggtaaggctc atggggggca accttggtcg tatccttcgc
    25381 tcaaatgctt ggaagccagc gcgctccaat cctccttata cggatcgggg ccggcttccc
    25441 gttgaacctg cgcgacgctt gcttccgttt cctgcaccgc ttcattacgc cacgcagcca
    25501 gcgtgggggt atccacccag ccctcttcca ccaggaaact ctccgcgact ttcaaacaat
    25561 cccgccccac aggggattgc ttcaaattcg ggtcaatgta gcctgcgtcg tcatgttcgc
    25621 cgtggccgca aagccggagt aactgcgcca caaccatttg cggcccgttg ccagcccttg
    25681 cccggttgac cgcgcctcct attactcgga ggcaggcgga taaatcggtg ccttccaccg
    25741 cttcgccctg aataccgtaa cctgcggcct tgtccagcaa cgaagcgcag gcaaattgcc
    25801 gctccgtggg agtggagtat gcgtattgat tgttggcgat aatcaaaacc aggggcagct
    25861 tttccaccgc cgcctgattc actccttcgt gaaaagagcc agtggaagtt gcgccatccc
    25921 ccacactcgc cactcctacc gtgtcggaaa ttcccttgaa ccgatgggcc agcagcgcgc
    25981 cattgaccat ggaaatcatg ctgcccagat gactgatcat cgcaaacacc ccttcccgag
    26041 gccgtccgcg atggacattt ccatctcgtg cgcgcatggg gcccaacggc gatcccaggt
    26101 aagtccgtac ggcgtccaga atcggttcgc caaaagccag ccgcccggct ccatcgcgaa
    26161 taagtggtgc gaaaatgtct cccttacgca aggccagtcc tgcggcgaca cttaacgctt
    26221 cctggccgcg gccgaggaag actccgccgt gaatcttacc catgcgataa aggctcgcga
    26281 acttgtcgtc caggacgcga gcgagcaaca tcacgcggta ggccttcaca taaagatcac
    26341 gaaaggagga atccccgcta accgcgtggt ggctgagctg agcatgagcc atacacggga
    26401 ctgtatccgc ctcggtatct tctggcaatg tttctttagt gtcttgaaac atggcagatg
    26461 catctcagtt tcagacaccg tcattaaatg agaactttgc ggcaatggtt tcggtatttc
    26521 ctggacagac tgccgcgcgt tcttctgccc ccgaaggttc gccacatcgg aacgcttatt
    26581 cagactgaaa tacaaccgat tcctttgcca ttcgtccttg cctcctctcc cggccatcgt
    26641 tttattgccg tagcatcact gaacccgctc cggattgtct cggggggtgg ttgggggaaa
    26701 cgtgtatatc aaagtaaatg ttcagaagta gcgtgcagac agatagttac gcagaggaac
    26761 aaaataagcc aaccctcgtt tcggtgatcc ttttcaatcc cgcttgggaa ggtaaccagg
    26821 ggccttcaat cgtgttgcga tttgtttcac ggcgacagaa accattttca aattatctgt
    26881 atctctgaaa acttacccaa atgggcaaag tgttcgatta gggcctttgt ttagttttag
    26941 gtaagcgtcg catatcaccc tcagctaaac cggcatgaaa tgccaggggt ggccgggcca
    27001 ccggggagat aatcgggttg gtacgggccg cgaattggtg ttgaatgtgc ttaatacaga
    27061 ccggtttatt ggatgtttca ttgcagcgaa taataagatt ctaggaccgc cggactatga
    27121 acacgcctgc gtctcaggta ttagtagtcg aagatgatcc gcgtttgccg gaggtgctgg
    27181 cgagcctgtt gcgagacgag aacattaccc tcgttactgc caccaatgcg gttgctgcct
    27241 tgcggttggc gcgtacgcgg ccattcgatt taatcttact cgatctcggc ttgccggatg
    27301 ccaatgggtt tgagctgttg cggcaactaa aggagtctcc cgcgacccaa cccatccccg
    27361 ttattgtttt gtccgcgtgg aacagcagca gcgacaagtt gcgcgggttt gaactgggcg
    27421 cagtggatta tctgacgaag ccgtttgagg gaggagagtt gcgagcccgc ctgcgtgccg
    27481 cgctgcgcac taaacatctg caagacgaac tgacgcaagc taatcacgaa ctgctggccg
    27541 cccgtttggc cgcggagacc tcggcacggg ccaaagcgga gttcctggcc aacatgagcc
    27601 atgaaatccg cactccgatg aacggtatca tcgccatgac cggtctgctt cgggaaaccc
    27661 cgttaacttc cgaacaacgc gggtatttgg agacgattta ttccagcggc gagtcactgc
    27721 tcaccatcat caacgacatc ctggacttct cgaagattga atccggcaag ttcgaactgg
    27781 agagccagcc cttcgattta tatgggtgtg cggaagaagc gcttgatctg ctggccccga
    27841 aagctgcgga aaagaaactg gaactggttt gtatgtgggc ggacgaggtg cccgagcggg
    27901 tgtgtgggga tgtcactcgc ctgcggcagg tgctggtcaa tctgctgagc aatgccgtca
    27961 agttcactgc cgccggcgag gtcgtaattg aggcggaagt tttatcggct cctctgcgcg
    28021 ccgaaaaaaa tcgggagccg tggcaactcc attttagggt gcgtgacacg ggcatcggta
    28081 ttcccgtgga ccggatggcc cggcttttta agtctttcag ccaggcagac gcttccactt
    28141 cgcgctgtta tggcggcacc gggcttggcc tggctatcag caaacgcctg gtcgagttga
    28201 tgggaggcaa gatgtgggtt gagagcgtgc tgcaaaaagg gtcaaccttc catttcaccc
    28261 ttcctttgcg caccgcgagc gaccaaccgc cgacgccagc caaaactccg ccgccgcaac
    28321 tcgccaacgt ccgggttttg atagtggacg acaacgccac caactgccga tttctcagcg
    28381 cgcaggcgcg caaatgggga atgaagccgc gttatacaca acgccctttg caggcattgg
    28441 aatggctgcg tgcgggcgaa ggcttcaaat tggcgattat tgatcaggat atgcctgaca
    28501 tggaaggaat caaattggcc gcggaaatcc gccagttgcc caaacaaggc aatctgccgc
    28561 tcgtgctgct caacccgccg ggctttcgcg ttgaaggtca cgatggcgcc aacgcgtcac
    28621 ctgtcaatgg gttggtcaag ccagttaagc ccggtcggct tcaggaactc ctggttcgca
    28681 tcatatccgg ggctcagccc gccgctcccc gagcgcagtc ttcccccaag gccgacacta
    28741 cgctggcgca ccgtttgccc ttgcgcgttc ttttgtgcga tgacaaccag attaatcaga
    28801 aggtagcctt gcgtttgctc cagcaaatgg gctatcgggc cgatctggcg gtcaatggtt
    28861 tggaagctct ggccgcgttg gatcgccagc cctacgatct gattttcatg gatttgatga
    28921 tgcccgagat gggtgggttg gaggctgccc gcgccatccg cgagcgtcag caacggcccg
    28981 cacaattccc tcattacaag tctccgatta ttattgtggc catgacggcc agcgcgatgc
    29041 cgggggatcg tgaaaaatgc tttgccgcgg gcatggacga ttaccttgcc aagcccgtgc
    29101 ggctggaaga cgtgcgcgcg attgtggaac ggtggggacc ggtcgcaggc acagctaaaa
    29161 gtgaagcctc cgctgcggca aaaactcccg tctccgcagc ggcgaaagag ccggcctcgc
    29221 ccgccggttc gccgccggtg ggctctagcg cgcccgtgga catggcaagg cttctcgact
    29281 ttacggatgg cagccctgaa aacctgcgcg aactggtgac cctttatctg gaccaaacca
    29341 gcgcgcaaat cgagcaaatc gaggccgccg tgcgtgacgg caaagcccag gaagtgcggc
    29401 gcctggcaca cagttgcgcc ggggcaagcg ccacctgcgg aatgacgaat ctggtgccgc
    29461 tgttgcgtga gttggaacgc ctgggtgcgg agaatgttct ggcgaatgcc tccgaattgg
    29521 ctcgcagagc gggcattgag ttcctccgca tccgggaatt tctggaggct tacctggtcg
    29581 agcacgccag cctcgctgct cggccttagc catgaaaaaa ttactcatta tcgaggatga
    29641 tcaggtcgta gcgaatattt accgccacaa gtttgccgtt gaaggttatc aagttgacgt
    29701 tgccctggac ggactggctg ggctggaaaa ggtccgcctt tttcgacccg acgtagtcat
    29761 tctcgacttg ctgctgccgg gagtttcagg ggtggaagtg ctcaaacagc tccgcgccga
    29821 gacggagtat gcaaagttgc ccgttgtcgt cttttcgaat acctacttat cgagcatcat
    29881 gcaggaggcg tggaaagcag gagccaccct gtgtttgtcc aaagccaatt gcagtccccg
    29941 gcaactgatt gacggggtgc ggaatgcgct ttttcctaat gacgcgtcag ctaccctttt
    30001 cgcgccccca accgcggctg ccggggccat tgttagttca ccgctcacgg cggaatctcc
    30061 ggccgccgaa acccatcccg aaaccaggga tcgagacgcc gcctttcaag ccgaccttcg
    30121 caattctttc attaccagtt tcccggccac gctggcaacc ttgcgctcac tcctcaggga
    30181 actgaatcag gccggagacg agactgcccg cctcaagccg ctcggggagt tatatcgtcg
    30241 cattcatgct ttgaccggca acgccggcat tgcgggcgtg ccccatgttg cccgcttgtc
    30301 tgacgcgctg gaagctctgc ttaaggaact ttgcgaaacg ccccggaaca tcaatgcctc
    30361 caccctgcgc acggtcgcgc tggcggttga cggtttggct ccgcttggtg aacagagtgt
    30421 tccgccgaac caaccgcaaa cgtcggcgcc cagggttctg gttgtgggcc atgaaaccct
    30481 ctcgcgacgc gctttggttt acgctttgga gaaggcaaag ctcgattcgg tcaatataga
    30541 tgattcgatc gcagcttacg aattgctggt cgggaacagc ttcgatttga tctttctcga
    30601 cgttgacctg cccggcctga acgggtttga actcagcgcc cggcttcgtg cattgccggc
    30661 gcaccaggca acccccgtgg tgctggtcac cagcctcagc gactttgaaa accatgcaaa
    30721 cgcttcgcag aacggaggca acgactttat aggcaaaccg tttctattca tagaggtggc
    30781 cgtcaaagca ctggctcatg ttcttcgcgg gcctctcttt gccgtgcaat aagattttga
    30841 ccctacgcac acgctggaaa cgcctttgcc ccaaggttgc ggcttaaaac ggaacgattc
    30901 ggttgggtag cccaaccgcc cgctcgcgtc ccttcggagc cagggtaact ctgatcgttc
    30961 aggccatagc catggcgaat cccttatttg gcgaattcaa tggcatggct gttgaatccc
    31021 aaacgtcttt tgtccgtgaa atcaatggct tacgtacacg aagaggaatg aatcgaaggc
    31081 ggaatctaaa attgttatgg cttaggccta ctatggccgc gccttggcta ttccctacct
    31141 ctaggccgtg tacaaggggt gtacaaggga tgctcatctg atgtaccaac taaaatccta
    31201 tgtattcctg gtgaacatcc cttgtacacc ccttgtacac gacctattga ctctcgcata
    31261 aataagtaac acaatgctgc cgcatgagca gcaaaac
//
LOCUS       DAOPQG010000033        31135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1896_length_31135_cov_13.748198, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000033 DAOPQG010000000
VERSION     DAOPQG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 31135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31135
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1896_length_31135_cov_13.748198"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..1725
                     /locus_tag="PKI07_05450"
     CDS             409..1725
                     /locus_tag="PKI07_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC50532.1"
                     /translation="MLDNLLDYIPDGVFTVDAEWRVTFFNRAAEQITGIRRAEAVGRR
                     CCDVFRASICESACALKQTLSTHRPVVNKVVYIIDARGQKIPISISTAAMKDGRGRVV
                     GGVESFRDLRVVETLHRELQQQNSFADIIGRSAAMQQVFALLPEIAESDSAVLIEGAS
                     GTGKELFARALHNLSRRRSKRFVALNCGALPDNLLESELFGYKAGAFTDARRDKPGRF
                     ALAEGGTIFLDEIGDISPAMQARLLRVLQERVYEPLGGVEPVKADVRFITATNKNLGQ
                     LVEQGRFREDLFYRIHVIRLELPSLRDRREDIPLLIDHFIARFNRLKGKDIGGVSDAV
                     LARLMEHDFPGNVRELENIIEHAFVLCRGGVIEMKHLPPPLRGSALGEALRLGRAMTL
                     KSMEKLLIQDALRRNRGNHQKAAQELGINPSTLFRKVKALRLAKRG"
     gene            1762..2082
                     /locus_tag="PKI07_05455"
     CDS             1762..2082
                     /locus_tag="PKI07_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOC50533.1"
                     /translation="MKLGIILSQTEPETVFNALRLANYSLQQGDRVKIFLMGKGVELE
                     QIRDPKFDVQGQARAVLDAGGQFLACGSCLKLRESAGSEVCPLSTLQDLYELVRDSDR
                     LVTF"
     gene            2151..2756
                     /locus_tag="PKI07_05460"
     CDS             2151..2756
                     /locus_tag="PKI07_05460"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC50534.1"
                     /translation="MAANRRLLLKREARLRRFGYDSRVGIQFVLAQALPLGGKVLEVG
                     TGKGRFLIRLARHVKAVVSVDVSAEEQRCARLNARNAGVERKIQFVLRDARRLPWANE
                     TFDAAVTMNAMHHIANFGQVLREMLRVVKPGGKIVLADFSPRGFQIIARAHRDEGRTH
                     PREFYDFRALAQTLRKRGLATRLRKGANQEVLIGRLPAAAA"
     gene            2975..4684
                     /locus_tag="PKI07_05465"
     CDS             2975..4684
                     /locus_tag="PKI07_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50535.1"
                     /translation="MITQPVSTDANPRARAWGYLTEVLSRAGLFYETLPASQVESLFR
                     RAKSVVILAGDLPLTQPQRAALAQWVKEGGALIGLGGASGLEEVFGVAGGIPVAEGWI
                     KVTAGEHPLTAGLRSSLHVFGGRALKKSSATVLAELQTGNGISKGSAILENRFGTGRT
                     VLLGPDLLFSIVHIQQGLRVLQDGRPAPDDSAPVNEGLLKTEDGLVLDWQRDRSPVPP
                     DGGPLFLEPISDELRELILRSVFHLARQQAILLPVLWYWPRSLKAVAQLSHDSDGNDP
                     QKAAALLEVMNRSRLKSTWCILYPGGYPVEFYRTLVEQDFEVALHYDAMTGGEHTAWS
                     RDNFLFQRRWLMKEAGLEQITTNKNHYLRWEGRLDFLRWCESAGIASDQSRGPSKKGT
                     IGFPLGGSQPYFPLDDEADSPRFLNVLEVNLLTQDLVITCPPEYGQPLLDSAARHHGV
                     AHFLFHPAHILKPGVADALAGLVDYGRRQGLEWWTNRQIWQWEMARRSVEARFHSGNA
                     ITLRAAASLPQATLLLLRSHPASCALALNGRPADPKPWDLYGFEFDALTVDIGDEIRV
                     QTI"
     gene            4714..5013
                     /locus_tag="PKI07_05470"
     CDS             4714..5013
                     /locus_tag="PKI07_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50536.1"
                     /translation="MLSDHDQKILPRCPRLGHEITFGYCRRETGGRPCRLILNCWWEQ
                     FDVRGFLQAHLPEDEMAQVEQAHAAAPPAKIVSLMEIIDQAVQRISGEKPGGSSG"
     gene            5152..5598
                     /locus_tag="PKI07_05475"
     CDS             5152..5598
                     /locus_tag="PKI07_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOC50537.1"
                     /translation="MNPALGRVVLIAVPNCQGRVSPVFDVAARLVLIRLKGEAEVERK
                     EIVLFEKQPEGMLHSLQECGVKLVICGAISRELQMKLEKAGIRVVPQICGELETVITA
                     FRRGTLGQREFTMPGCCGRQWGAGRWRGRCRRGPHGKARGNYAAWT"
     gene            5644..6006
                     /locus_tag="PKI07_05480"
     CDS             5644..6006
                     /locus_tag="PKI07_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOC50538.1"
                     /translation="MKIAIPLTGGRLSAHFGHCEQFALIEADLASKQILTQTRVAPPP
                     HEPGLLPRWLYLQGVQTVIAGGMGRRALDLFAQNGIAVHAGAKDDAPEKLAQAFLNGT
                     LSGGTPTCGHDHKHTHCS"
     gene            6003..6362
                     /locus_tag="PKI07_05485"
     CDS             6003..6362
                     /locus_tag="PKI07_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOC50539.1"
                     /translation="MKVAITTQGPDSASPVDPRFGRARFFRIVDTESGQQTTVDNAEG
                     MNAVQGAGTQAVQALARLGVQAVLTGHVGPKAWNALQAAKIQMYSVTSGSAEAACQAF
                     LAGQLSMLTPAEAPGHA"
     gene            6581..7639
                     /locus_tag="PKI07_05490"
     CDS             6581..7639
                     /locus_tag="PKI07_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC50540.1"
                     /translation="MAAAVGAAGRKDKTSGRQTLQPKSSLVENQDALLAVRLAIASGK
                     GGTGKTTIATNLAMRLAHHGQEVVYVDCDVEAPNGHLFLKPDEVREYPVNRLVPQVSQ
                     ERCQHCGDCGRICRYNAILCLAERTLIYDELCHSCGGCVRVCPTRAITEIPHFVGVVR
                     IGIAGKVQFVSGTLKVSEASSPPVIRAALQAVPPSDWVILDAPPGAACSVVETLRDCD
                     YVLLVSEPTPFGLHDLQIALELAKTLAVPCGVVVNRAYSSMAELRELCGQAQVPVLGE
                     IPDDLAIARAYSRGQRIIDVVPGLHRTFDHLLLRLLEELQPNVLPFRTRQDLEQAIYA
                     NDRMEATNLNEPGRLMAT"
     gene            7636..8286
                     /locus_tag="PKI07_05495"
     CDS             7636..8286
                     /locus_tag="PKI07_05495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC50541.1"
                     /translation="MKDEASKNMQSVSGTMRKYWKSCESEFWQNVFRVETDYLLKHLK
                     GCQTVLSVGCGPAVIEAALGKHGYQVTGVDIEPGALREASGAVKMVVARAEDLPFPAA
                     AFEAVIFVTSLQFIEDYRAAVVQAARVLRESGQLIVMLLNPASAFFQRKLCQPHSYVH
                     WIRHTDLEEIQSVISEQFEAQTEYFLGIDGATLFETRDPAGAALFVIRGTRKAASL"
     gene            8283..9212
                     /locus_tag="PKI07_05500"
     CDS             8283..9212
                     /locus_tag="PKI07_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC50542.1"
                     /translation="MTELVVLSGKGGTGKTSVTASLAALAEAPVLADCDVDASNLPLV
                     LAPRIQHHGTFSSGLRARIQSSRCSVCGQCEELCRFNAISYYESANDDEAGAFRVDPI
                     ACEGCGVCAHFCPEGALEMIPSDGGEWFLSETRLGPLVHARLRPGQGNSGKLAALVRE
                     KARETARANGRELVLIDGPPGIGCPVIASMTGATNVLVVTEPTVAGKYDLERILALIR
                     HFNLPVSVCINKYDLNSAMVAQIERYTAELGATTVGRIPYDPAVTAAQRRGISVVELN
                     QGQAAPAIVELWKTLSEKCLNLTPGHYQPISAG"
     gene            9432..10139
                     /locus_tag="PKI07_05505"
     CDS             9432..10139
                     /locus_tag="PKI07_05505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOC50543.1"
                     /translation="MTTASVAPETTSAARIPLIGETAPAFEAHTTQGPISFPKDYAGK
                     WVILFSHPADFTPVCTTEFMTFATMMPEFKQLNCELVGLSIDSHYAHIAWLRTIKEKI
                     KYKNMADVEIVFPVISDVKMEVAKAYGMVQPGASSTEAVRAVFIIDPKAIIRAVLYYP
                     LSNGRNFPEIKRLLIALQTTDRHGCATPADWQPGDDVIVPPPGSCGTAKQRVEKPAPD
                     AYALDWFMTFKKLPKDN"
     gene            10206..10571
                     /locus_tag="PKI07_05510"
     CDS             10206..10571
                     /locus_tag="PKI07_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOC50544.1"
                     /translation="MNVAVLGASNKPERYSFKAVRMLREHGHTPLPIHPALAEVDGLP
                     VFPSLEMVSTPIDTITVYLSPRNQQPIVKDLLSSGARRVIFNPGSENPSLADQLRQSG
                     KQVVHACTLVLLATGQFAS"
     gene            10682..11554
                     /locus_tag="PKI07_05515"
     CDS             10682..11554
                     /locus_tag="PKI07_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOC50545.1"
                     /translation="MTLPTALLLGYCLLALLAALIGGMMPSLIKLTHTRLQFAVSLVA
                     GLMLGLSVLGLLPHATHELGSVHQSTSWLLAGFLVMFLLQRFAPFHHHDVPEGSPPEP
                     CGHTHSLAERSARGLSWIGLAFGLSLHSVFDGLAMAAAVTADAQTPRGGALGLGTALA
                     VILHKPFGALAITTLMTASGAGRALRNWVNLAFACVTPLGAMLFFLGAGPWAVVHPSW
                     LGCALAFCAGTFLCIACADLLPELQFHTHDRFKLSLALIIGLGIAIFIGQLKLPGHGH
                     SSNHPKTEHLHPPR"
     gene            11551..12015
                     /locus_tag="PKI07_05520"
     CDS             11551..12015
                     /locus_tag="PKI07_05520"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOC50546.1"
                     /translation="MSSTFFERGGLWMLGQNLLLVFVGAGGFFYRNHWHHVLLARCGA
                     FLLLFAAGCGLAGAISLGRNLTPFPRPSQSGRLVRTGIYQFVRHPLYVVVFCASVGWA
                     LFCASWPALLAALVLAPVLDAKARREERWLQQRFPEYASYEQRVRRFIPWVY"
     gene            complement(12038..13498)
                     /locus_tag="PKI07_05525"
     CDS             complement(12038..13498)
                     /locus_tag="PKI07_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC50547.1"
                     /translation="MLLHLRFPLLPKILIWLLLNLAVLGAAIHLVVRLQFQFGLDSLL
                     AGRAGGRVQAVSDVITDELKAKSVSSWDEVLQRFGKAYQVQFFLFRPDGSQVAGATMD
                     LPTEVAAKVAERPNPGAGVRRGPPPGRGPRWTREESDVRPEARFVVRTSNPTGYWIGI
                     RLPLAEPGTSRPWPLTLLLNSSSLSAGGLFFDPTPWVMVGVGALVFSVLFWLPLIRGI
                     TRSLSQMTRATEQISEGWFETRVNDRRGDELGRLGLAINRMAVRLAGFVTGQKRFLGD
                     IAHELCSPLARIQVALGILEQRTDDKQQTYMHDLREEVEQMSRLVNELLSFSKAGLTP
                     QAVQLEPVNLAATVQRVLEQEAAPTLHLHVQVPDGLTVMAQPELLFRALANLIRNALR
                     YADPAGPIIITAERKDDTVIIGVADEGPGIPEESLPQVFDPFFRGEPSRSRATGGTGL
                     GLAIVKACVEACRGTVSAKNRKPRGLEVLMTLSVPT"
     gene            complement(13511..14254)
                     /locus_tag="PKI07_05530"
     CDS             complement(13511..14254)
                     /locus_tag="PKI07_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC50548.1"
                     /translation="MLTEGARRPQAGEIKPRLLIIDDDKKLCRLIRDYLEPMGFEVEA
                     AHTGPEGLEKAQTGQFQAIILDVMLPAMDGFEVLRLLRRTSDVPVLMLTARGEEPDRI
                     VGLEIGADDYLPKTFSTRELLARLRAVVRRSSRTVVSAQPQNEPEIQVGALRVIHASR
                     VALLANQPLTLTPVEFDLLAALARARGRVKSREQLLEEIRERHYDVFDRSIDVHVSTL
                     RRKLGDDARDPRYIRTVRSAGYMLINPDA"
     gene            14453..14971
                     /locus_tag="PKI07_05535"
     CDS             14453..14971
                     /locus_tag="PKI07_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50549.1"
                     /translation="MKQTITLVIGLVLAGGVLTVGAQPGQGRGAGAQHRRGAQQFPPP
                     IAVTFDIDLDGVLSAAEIQQAPSRLLKLDQNGNGQLEQQELCSGGGGPGGQTCMWGGQ
                     GIGRRANGNGQNAVLLALFDSNKDGLLDSAEINGASAVLKALDKNNDGQVTAAEIRPL
                     PGRGQGRPWAQK"
     gene            complement(15115..16299)
                     /gene="tyrA"
                     /locus_tag="PKI07_05540"
     CDS             complement(15115..16299)
                     /gene="tyrA"
                     /locus_tag="PKI07_05540"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HOC50550.1"
                     /translation="MSKPRSKPCLPGPTPSGSAQGAKSALDSLREQIDGIDQQLVDLL
                     VQRGRIIQQVTDIKKRHDLPTFHPAREENLISTRRAQATQAGLDPDYVEDLFRTMLRH
                     SRVGQLSTLSRRSVRPGAKILIVGGLGNMGRFFSTWLRQSDYEVRVLDRDDWPKVRSL
                     TTGIDLCLLAVPIEITPSIALQIGKYLPPACVLTDITSLKRKPLEAMLRGHAGPVVGL
                     HPLFGPATVTMDKQIVVFSPGRSLSKCQWFLDQLTLWGNVLVETPASEHDEIMGIVQA
                     LRHFATFTFGQFLCSRGVPVLRTLELSSPIYRLELAMVGRLFAQDPQLYAEIIFATPE
                     RQALLKDYLESLQKNLSLVERGDKTEFIARFRQIAEWFGPFSEQAMRESTFLVEKLVH
                     RF"
     gene            16581..17135
                     /locus_tag="PKI07_05545"
     CDS             16581..17135
                     /locus_tag="PKI07_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50551.1"
                     /translation="MALAGYRLVGNTKMTAAKVKEYAESVDISQLKDDARAKAIQGLA
                     DRLNALSLEERRKARLEQEAWNWFDQMTEAERAGFIEATMPTGFKQMLVSFEQLPDDK
                     RRKAVDDALRRLKEARARAGLSLGRRGTNSPPVLSDELEAQVRKIGLKTFYSESSAQT
                     KAELAPVLEELQRVMESGRPFRGR"
     gene            17162..17821
                     /locus_tag="PKI07_05550"
     CDS             17162..17821
                     /locus_tag="PKI07_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50552.1"
                     /translation="MNSAVEQRTGPGSARGTRSRQETIRGWFAGAFTLIELLVVIAII
                     AILAALLLPALSRAKESGQATVCLSNLRQIGLALQIYVSDNHNRLPTMRDRSLTTLTN
                     ELPSPDLVLSNYVGNLNVFKCPSDKWPGDTAKLLPGAGATFYEQTGSSFAWNSLLNGR
                     DAEHLVAFGLKFDPHQMPLMYDKEKFHIARGAAKAQNWLYADGHIKNLLLFEGTIRKY
                     P"
     gene            17818..18732
                     /locus_tag="PKI07_05555"
     CDS             17818..18732
                     /locus_tag="PKI07_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC50553.1"
                     /translation="MSEPMIQLKRLNKQFGARQAVNNLTLAVPAGEIYGLLGHNGAGK
                     STAIGMMLGQVWPTSGEVLICGYDVSAHRRQALQKVGAIFESPAFYDYLSAVRNLEIL
                     SSYTALVSPRRIQEVIEWVGLSGRERSLVRTYSHGMRARLALAQALLPQPELLILDEP
                     SDGLDPEGIHEMRQTILRLRQELGLTILLSSHFLHEVEQLCTRIAVLNQGWMVFEGAL
                     AATKTNEIWVRLKTKDFGLAVKELLQARMITRDRDECMIALSPGVETDQIVRRLVERA
                     IPVYEIVPAEQTLEDFYLSLMERQRKAQ"
     gene            18750..19571
                     /locus_tag="PKI07_05560"
     CDS             18750..19571
                     /locus_tag="PKI07_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOC50554.1"
                     /translation="MRNELWKLFGKKRTYIGFGAFLLAQNAMLLAFKFTRWQSEMERL
                     LEGNGYLALEYVSALTVALIMLIPQIALLMPLYTALVGGDLVAKEVEDGTLRMILSRP
                     ISRFRLLLVKWLAGGIFSAALVVMLGVTALGFARLWFPWQGMFVFIPGQIFNVLSPAD
                     GLRFYAFSHLFMTLNACTVLGIALMFSSFNIKPAAATILALSLLFANLVMEGIPFFER
                     YHQYLLTFHFHAWRLVFSQPMPWAQIAQSVCVLLAVNLTTFLIAAAAFQARDIKS"
     gene            19782..22289
                     /gene="gyrB"
                     /locus_tag="PKI07_05565"
     CDS             19782..22289
                     /gene="gyrB"
                     /locus_tag="PKI07_05565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOC50555.1"
                     /translation="MPVENTVEPTGKLAAISSAPEKYDASKIDKLEGLEAVRKRPGMY
                     IGDPDERGLHHSVFEVLDNSIDEHLAGFCSKIEVAVHVDGSVSIRDNGRGIPVDMHPK
                     WNMPAVELVLTNLHAGGKFGQGAYKYSGGLHGVGAKCVNALSEWFKVEVSRDGKVYSM
                     EFSRGVTTQKLTVVGKSRGTGTLITFKPDPLIFTITTEFKFDVLANRLRELAFLNPGV
                     EIVLTDERDEKTETFLYKDGIEQFVKQLGRTKQVLHPKPIVISRQKEEVFVDCVMQYT
                     DSYNDQILCFANSIANPDGGTHLTGFRSALTRAINQYAKQSNLLKEKDPSISGDDVRE
                     GLVCVLSVKLPNPRFESQTKVKLVNTEIDGLVSSVVYEGLMTHFDATPAMAKKVVEKA
                     LLAARAREAARKARETVRKGALTGGGLPGKLADCSDRDPVNTELYIVEGDSAGGSAKQ
                     GRDRKFQAILPIRGKIINVEKARLDKVLQNNEIRTLITAVGTGIGDGEGEGAFNLEGL
                     RYHKIIIMTDADVDGSHIRTLLLTFFYRQMPELIRGGFVYIAQPPLYQISRKKRTEYV
                     DDDARLNKILIELGTDEVRLRNLADGKELTEKQLAEILELLESLDKYANALRRHGGDF
                     ADYVEQRHPQTHELPRHLVKVRNGNEETVHYFHSEEELEEFGSANPDLRLFGEEDSDT
                     SLVEKNKNGHARRARHVELHESKAVAELLARLGQKGLSLEHYSAQDKPLFELIEGEGD
                     KEEIKPLFSIPEILSRVKEVGRRGLSIKRFKGLGEMNPKELFETTMNPARRKLLRIDL
                     TDAVEAEEMFVKLMGDEVEPRRQFIEDNALNVRNLDI"
     gene            22343..24985
                     /gene="gyrA"
                     /locus_tag="PKI07_05570"
     CDS             22343..24985
                     /gene="gyrA"
                     /locus_tag="PKI07_05570"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOC50556.1"
                     /translation="MPDDTPSNDPRPALTPAYAAHEKINKINVAEEIKNSFLDYSMSV
                     IISRALPDVRDGLKPSQRRILYAMHDLGVMPNRKHLKCAKIVGETMGNYHPHGDLAIY
                     PTLVHMAQPWAMRERLVEGQGNFGSVEGDPPAAMRYTEARLAPLGAVLMEDMDKDTVD
                     FVPNYDETRTEPTVFPAAFPNLLVNGGTGIAVGMATNMPPHNLGEIVDGICAQIDKPD
                     ITLRELMQHVKGPDFPTGCMICGLEGIKNYFTDGRGSLKVRGKVGLEEIKGGREQIII
                     TEIPFNVNRAVLVERIATLVNEKIITDITGVRDESDENTRIVIEIKRDAVPKVVINNL
                     YQYTALESSFSVNALAIDHGRPKTLGLKDLIGCYIEHRREVVIRRTRFELRQAEERAE
                     ILEGYLIALSNLDEFIRIIRHSHTREEAKIKLLAFDFTRAQVETLGILIRSEARLLNG
                     RYSFSEAQANAILELRLYQLTGLEIDKVRAEYRALIARIKDLLDILAKEERVLGIIKT
                     ELQAIKAKYATPRVTELVPDEGEIAIEDLIANEGVIITLTHTGLIKRTNISSYRAQRR
                     GGKGVIGMTTREGPTEEDVDFIEHLFTASTHDYLMFFTNTGRVYAERVHEIPDMGRAA
                     KGRSIANLLELKAGETIAALIRIEARTGPNKEDITWQQPGFLFFATQQGTVKKTPLTD
                     FANVRKDGIIAIRIEPGDALIDVKLTSGQDQVVLITRDGMSIRFAEEDVRPMGRPAAG
                     VRGINLEKADAVVALALAVPDATLLVAGENGIGKRTPFDLAREDGTTEPVYRLQSRGG
                     KGIITMKANEKTGAVVGALTVQDGDEVMLITAGGQLVRTYVKDIRETGRNAQGVRLIN
                     LAEGDQLQAIAPVISEQQENGAEE"
     gene            complement(25064..25771)
                     /gene="pdxH"
                     /locus_tag="PKI07_05575"
     CDS             complement(25064..25771)
                     /gene="pdxH"
                     /locus_tag="PKI07_05575"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOC50557.1"
                     /translation="MAIADLRREYNLTGLRRRDLEADPIAQFKKWFEQAAGVRASGRL
                     RKFGIKLYKKLLLASGAEPLDLTAMTLATVDSHGHPSARVVLLKGVDERGFVFFTNYE
                     SRKGQELAGNPRAALVFYWSNQERQVCIAGEISQLPPAESDAYFHSRPRGSQEAAWAS
                     PQSTVIPDRAALEEKWRQIEARYAGKEVPRPPFWGGYRLWPARIEFWQGRPNRLHDRF
                     RYTRQPDNTWRIERLSP"
     gene            complement(25856..26422)
                     /locus_tag="PKI07_05580"
     CDS             complement(25856..26422)
                     /locus_tag="PKI07_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HOC50558.1"
                     /translation="MQSVFAGPVFVVRDNIDTDQIIPAQYLNLVPTIPQEYEKLGAYA
                     FAGLPESAYPKRYVDEGQLDSQYPIVVAGRNFGCGSSREHAPIALGSAGCKVVLAESF
                     ARIFFRNAVATGELYPCETVERLCDALKTGEVVTVDLDAGTVTSKDSGKVYPFKSLGD
                     VRPVVDAGGIFNYARKTGMMPASSAAQP"
     gene            27037..27195
                     /locus_tag="PKI07_05585"
     CDS             27037..27195
                     /locus_tag="PKI07_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50559.1"
                     /translation="MADSHLHVDLVGKHLVHYKLIKRDAKRTPMSLSNKKTVEKYLRT
                     NKAVLVQG"
     gene            27469..28818
                     /locus_tag="PKI07_05590"
     CDS             27469..28818
                     /locus_tag="PKI07_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50560.1"
                     /translation="MNRRQFIQAGAAGLALASLGGYAAEFADQKKRVGLIGCGWYGKS
                     DLLRLIQVAPVEVVSLCDVDKQMLAGAAEIVAARQASRKAPRTYHDYREMLREKDLEI
                     VLIATPDHWHALPMIAAVEAGADVYVQKPISVDVVEGQAMLAAARKHKRVVQVGTQRR
                     STPHLIEARDAILREGKLGKIGLVEIYSYYGGGAGTEPEDSAPPDYLDWEMWTGPAPL
                     RPYNRMKHPRGWRSFMEYGNGTVGDLCIHMLDAARWMLGLGAPRTVSSAGGIFVRQGR
                     KANIADTQTAIFDFGDLQVVWQHRRWGQSPDPRYPWGITFWGEKGHLKASINSYTFVP
                     ADNRPPLHREVTMELEQYPEDREEKELEKQVAPAIRGHMKDFLAAIATRGKPVADIEQ
                     GYISTTACILANLSMKLGRSLAWDAARGRVVGDEEANGLLARPYRAPWEHPEPLKFS"
     gene            complement(28865..30370)
                     /locus_tag="PKI07_05595"
     CDS             complement(28865..30370)
                     /locus_tag="PKI07_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50561.1"
                     /translation="MNLNRRDFLKTVALGAAAFSLDARTWSRAVGANDDIRVAVIGFR
                     GQGAGHIKALNRLSGVRVVAICDVDRQVLEAKARELGSSVQACTDLRRVLDNKDVDAV
                     SIATPNHWHALATVWACQAGKDVYVEKPASHNLFEGRKMVEAARKYKRIVQCGTQCRS
                     SGGLQEAVQYVREGRLGRILLARGFCYKARRSIGTVDGPQPVPAYIEYDLWCGPAPNE
                     PPRRKTGNGTIHYDWHWFWNYGNGDYGNQGPHQLDIARWFLGEKTIAPFTQAIGGRLG
                     YQDSAETPNTLIVYHGYKTPLLFEVRGLPHDQAAQSQGWKMDNYRGVSIGNVIECEHG
                     SVVVPSYTEAIIYDRDGKEITRFTGRKKAAADSVKTPVGLTAESGGHHGNWIAAVRSR
                     NVQALNAEILEGHLSAGLVHTGNISYRLGAQKAPGEIRQALADNRSLAEAFDRMAGHL
                     DANGVDLAKDKVWLGMPLKFDPRKERFAGNRAANEMLTRPYRPPFVVPAKV"
     gene            30831..30992
                     /locus_tag="PKI07_05600"
     CDS             30831..30992
                     /locus_tag="PKI07_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50562.1"
                     /translation="MSSLLLKSDAQITQARQAVQAYRQVLAALDQLSWINLELLRRDE
                     PLFGKPTPV"
BASE COUNT         6511 a         8828 c         9500 g         6296 t
ORIGIN      
        1 cgggcgcaca cgccgaaggg ggattggtaa taggtttccg cgcccaggct gcccgtgcgc
       61 ggagcggata gtattttcat aacgtagttc ctttttgttg tttttttatt gtcgttctgt
      121 catcgccccg gagggcgact cggcttcctg ccattccttg ggctggaagt ccccgccgcc
      181 gccaccccga gcccacgcgg gaaggctggc gggtagggtg tctttgcgca agggcgaatt
      241 tgggaggaat tggcgatggc aggccggtgc agggcaccgt gtaagggcac gcggcctgca
      301 aggggcggca tcatcgcttt tagcgcctta gcgaatgttg gctgcaacac caacctggtt
      361 gttgagtggg gctggcgagg ggcggtctct ggatgtacac tagccgcgat gctggataac
      421 ttgttggatt atattcccga tggcgttttc acggtggacg cagagtggcg cgtgacgttt
      481 tttaatcgcg ccgcggagca gattaccggg attcggcgcg ccgaggcggt ggggcgccgg
      541 tgctgtgatg tttttcgggc gagcatttgc gagagtgcgt gcgcgttgaa gcagacgctt
      601 tccacgcatc ggccggtggt gaacaaggtg gtgtatatca ttgacgcgcg cgggcaaaag
      661 ataccgatca gcatttcgac ggcggcgatg aaggatggac gcgggcgcgt ggtgggcgga
      721 gtggagagtt tccgggacct gcgggtggtg gagacgctgc accgggaatt acagcagcag
      781 aattccttcg ccgacatcat cgggcgcagc gcggccatgc aacaggtttt tgcgctgctg
      841 cccgagattg cggagagcga cagtgcggtg ctgatcgaag gagccagcgg gacgggcaaa
      901 gagttgtttg cgcgcgcttt gcacaacctg tcgcggcggc ggtcgaagcg gtttgtggcg
      961 cttaattgcg gcgcgctgcc ggataacttg ctggaatcgg agctgtttgg ctacaaggcg
     1021 ggagcattca cggatgcgcg gcgcgacaag ccggggcgtt ttgcgctggc cgagggcggc
     1081 acgatttttc tggacgagat tggggatatt tccccggcga tgcaggcgcg attgctgcgg
     1141 gtgttgcagg aacgggttta tgagccgctg ggcggggtgg agcccgtgaa ggccgatgtg
     1201 cgttttatta cggccaccaa caagaacctg ggccaactcg tggagcaggg gcggtttcgc
     1261 gaggatttat tctaccgcat tcatgtcatc cggctggagc tgccgagcct gcgagaccgg
     1321 cgggaggata ttccgctttt aattgatcac ttcatcgcgc ggttcaaccg gctcaaaggc
     1381 aaggatatcg ggggtgtttc cgacgcggtg ctggcgcggt tgatggaaca cgactttccg
     1441 gggaacgtgc gggaactgga gaacattatt gagcacgcct ttgtgttgtg ccggggtggc
     1501 gttattgaaa tgaagcacct ccctccgccg ctgcggggca gcgcgttggg ggaagcgctc
     1561 cggttgggcc gggcgatgac tctgaaatcc atggaaaagt tgctgattca ggatgccctg
     1621 cgccggaatc gggggaacca ccaaaaggcg gctcaggagc tgggcattaa tcccagcacg
     1681 cttttccgga aagtcaaagc gttgaggctg gcaaagcgag gttgagcatg gcacacttaa
     1741 agcaaatggt ctggtcataa aatgaaactg ggcattattc tttcacagac agagccagaa
     1801 acggtcttta acgcactgcg gctggcgaac tatagtttgc agcaggggga cagggtgaag
     1861 atattcctaa tgggcaaggg agtggaattg gagcagattc gcgatccgaa atttgatgtt
     1921 cagggacaag cgcgggcggt tctggacgcg ggcgggcagt tcctggcctg tggcagttgc
     1981 ctgaaattga gggaatcggc agggtcggaa gtgtgtccgc tttcaacgtt gcaggacctg
     2041 tatgaacttg ttcgggattc ggatcggctg gtgacctttt gagcatgggg aggggcctga
     2101 aaacggaaaa ccggccgcag cgcccggcga aacgcgcggc agaccccgag atggcggcca
     2161 accgccggct gctgctgaaa cgcgaggccc ggctgcggcg gtttggctat gactcgcggg
     2221 tgggaattca gtttgtgctg gcgcaggcac tgccgctggg agggaaggtg ctcgaggtgg
     2281 gaacgggcaa ggggcgtttc ctgatccggt tggcgcgcca tgtcaaggcc gtggttagtg
     2341 tggatgtctc agccgaggaa caacgatgcg cccgcctcaa cgcaaggaat gcgggcgtgg
     2401 aaaggaagat tcaattcgtg ctgcgggatg cgcgccggct gccgtgggcg aacgaaactt
     2461 ttgatgcggc ggtcaccatg aacgccatgc atcacatcgc gaatttcggg caggtgctgc
     2521 gggaaatgct gcgggtggtg aagcccggcg gcaagattgt gttggcggat ttcagcccgc
     2581 gggggtttca gattatcgcg cgggcgcatc gggatgaggg gaggacgcat ccgcgcgagt
     2641 tctatgactt tcgggcgctg gcgcagacgc tgcgaaaacg cggattggcc acgcggctgc
     2701 gcaaaggggc aaaccaggag gtgctgatcg gccggctccc ggcggctgcg gcgtaagtat
     2761 ggtgaaacga gggccagccc caatcagggg acatgctacg cgacgtgact ttctccggcg
     2821 ggcggcgggc gcaacttcct gtctgctgat gcaaggattg ggcgtcgtgg gaggcgccgg
     2881 ccagacccgc gagcggcgaa cgaatatgcc agttccggtt tccggagcgg cagtgagtgg
     2941 ccgaccggga atagttccag gcgctccggt cacggtgatt acacagccgg tttccactga
     3001 tgccaatccg cgcgcgcggg cctggggcta cctcacggag gtcctcagcc gcgccggcct
     3061 gttttacgag accctcccag cgagccaggt ggaatcactg tttcggcgcg cgaaaagcgt
     3121 cgtgatcctt gccggcgatt tgccgctgac gcaaccgcag cgagcggcgc ttgctcagtg
     3181 ggtcaaggag ggcggggcgc tcatcggcct gggaggagcc tccggtttgg aggaagtatt
     3241 tggggtggcc gggggaatcc cggtggcgga ggggtggata aaggtcacgg ctggagaaca
     3301 ccccttaacg gcggggttgc gcagttctct gcatgtcttt ggcgggcgcg cactgaagaa
     3361 gagttcggca accgttctgg cagaactgca aacgggcaac ggcatttcca aagggagcgc
     3421 catactggag aaccgcttcg gcacgggccg aaccgttttg ttgggcccgg atttgctctt
     3481 ctcgattgtt cacatccagc aggggctgag ggtgctgcaa gatggcaggc ccgcgccgga
     3541 tgattcggct ccggtgaacg aggggctgct caagaccgaa gacggcctcg tattggattg
     3601 gcagcgggac cgatccccgg tgccgccgga tggcgggccg cttttcctgg agccgatcag
     3661 cgatgagttg cgcgaactca ttctgcgaag cgtttttcac ctggcccgac agcaggcgat
     3721 cctgctgccc gtgttgtggt attggccgcg ctcactcaaa gcggtagcgc agctatcaca
     3781 tgactcggat gggaatgacc cgcaaaaggc ggcggctttg ctggaagtga tgaatcgcag
     3841 ccggctgaag tccacttggt gcatcctcta cccgggaggc tatccggttg aattctaccg
     3901 cacgctcgtg gaacaagatt ttgaagtcgc gttgcactat gatgccatga cgggcgggga
     3961 gcatacggca tggtcccgag acaactttct gttccaacgg cgctggttga tgaaggaagc
     4021 cgggctggag cagattacta ccaacaagaa ccattacctg cgctgggagg ggcgcctgga
     4081 tttcctgcgc tggtgtgaat cggcgggcat agcttctgat caatcgcgcg gaccgagtaa
     4141 aaagggcacc attggctttc cgttgggagg ttcgcagccc tactttccac tggatgatga
     4201 agccgattcg ccgcggttcc tcaacgtgct cgaggttaac cttctgacgc aggacttggt
     4261 cattacctgt ccgccggagt acggccaacc cttgctcgat tccgccgcgc ggcaccatgg
     4321 cgtggcgcat tttctttttc accccgcgca catactcaag ccgggggtgg cggatgccct
     4381 tgcgggcttg gtggactatg gccggagaca gggattggaa tggtggacca atcggcagat
     4441 ttggcagtgg gaaatggcgc gccggagcgt ggaggcccgc ttccattccg ggaacgccat
     4501 cacgctgcgg gcagccgcca gtcttccgca agctacgctg ttgctgttgc gctcacaccc
     4561 cgcgagctgc gctcttgctt tgaacgggcg cccggcggac cctaagcctt gggacttgta
     4621 tgggtttgaa tttgacgcgc tcaccgtgga tattggggac gagattcggg tgcaaacaat
     4681 ctgagaacca atgaaataaa ccccgctcgc ggaatgctca gcgaccacga ccaaaagatt
     4741 ctgccccgct gcccccgcct tggccacgaa atcaccttcg gttattgccg ccgggaaaca
     4801 ggcggccggc cctgccggtt gatcttaaac tgctggtggg aacaatttga tgtgcgcggt
     4861 ttcctgcagg cgcatttgcc cgaggacgag atggcgcaag tcgagcaagc gcatgccgcc
     4921 gcacccccag ccaagattgt cagcctgatg gagataattg accaggcggt gcagagaata
     4981 tcgggcgaaa agcccggcgg gtcttccggt tagcatggca aaatgcaacg ggcggcgcag
     5041 gtagctattg cagtctgcgc tgcttccgga tcattgcccc agcgggtcgc catgcccaaa
     5101 agcctgatta taaaggagat ttagtatgaa acgaccgggg ggtggcttgg catgaatccg
     5161 gcattaggga gggtcgtgct gatagcggtt cccaactgtc agggtcgcgt gtcgcccgta
     5221 ttcgacgtgg cggcgcgact ggtattgatt cgcttgaaag gcgaggccga agtcgagcgc
     5281 aaggagatag tgctctttga aaagcagccg gaagggatgc ttcacagcct gcaagaatgt
     5341 ggggttaagc tggtgatctg cggggccatt tcgcgggagt tgcagatgaa gctggaaaag
     5401 gcgggcattc gagtcgtgcc gcagatttgt ggagaattgg agacggtcat tacggctttc
     5461 cgacggggca cgctggggca gcgtgaattt acgatgccag ggtgctgcgg ccgacagtgg
     5521 ggcgccggcc gatggcgggg ccgatgtcgg cggggacctc atggcaaggc tcgggggaat
     5581 tatgctgcct ggacttaaac tggcggtgaa acagactgcg attttattag gaaacacatt
     5641 gatatgaaaa tagcaatccc tctaacggga ggccgattgt cggctcactt tgggcactgt
     5701 gaacagtttg ccttgattga ggctgacctt gcctccaaac aaatcctgac ccagacccgg
     5761 gtggccccgc cgccgcacga accgggttta ctgccccgct ggctctatct ccaaggggtc
     5821 cagacggtta ttgccggggg catgggaagg cgcgcgttgg atttatttgc ccaaaacggc
     5881 atcgcggtac acgccggcgc gaaagatgat gcgccggaga aattggcgca ggcgttttta
     5941 aacgggacgc tgagcggcgg gactccgaca tgcggccatg atcacaagca cacgcattgc
     6001 tcatgaaagt cgcaattact acccagggac cggattccgc ttcgccggtg gaccctcggt
     6061 ttggccgggc gcggttcttt cgcatcgtgg ataccgagag cggacagcag acgacggtgg
     6121 acaatgcaga agggatgaac gcggttcagg gggctggcac gcaggccgtt caggcactgg
     6181 cccgtctggg ggttcaagcg gtccttactg gacatgttgg cccaaaggcc tggaacgcgc
     6241 tgcaagcggc caaaatccaa atgtattccg tcaccagcgg ctcggctgaa gcggcttgcc
     6301 aggcctttct cgcgggccag ctttcgatgc tgacgccagc ggaagcgccg ggacacgctt
     6361 gaggcaaagc aggctgcccg cctccaggcc gggcgaaaac caaacttcat cacggaaaga
     6421 aaccaagcta gaaaggacaa agttatgaca ccaatgggag atgggaccgg cccgttcgga
     6481 caagggccag tgggtaagag gatggggcct tgcggcggag gaaggcgttg cggcagaggc
     6541 caaggcagtc aaggccgagg gcggggtggc ggtcgaggtt ttggccgccg ccgttggcgc
     6601 agcgggccgc aaggacaaaa cttcagggcg gcaaacgctc caaccgaaaa gcagtctggt
     6661 tgagaatcaa gacgctctct tggcagtgag gcttgcgata gccagcggca agggtggaac
     6721 gggcaagacc accattgcca cgaatttggc gatgcgactg gcgcaccacg gccaggaggt
     6781 ggtgtatgtg gattgtgatg tcgaggcacc caatggccat ctctttttga agccggatga
     6841 ggtccgggaa tacccggtca accggctcgt tccccaggtg agccaggaac gatgccagca
     6901 ttgtggcgat tgtggccgaa tttgccgtta caatgccatc ttatgcctgg ctgagcgaac
     6961 gctgatttat gacgagttgt gccacagttg cggcggctgc gttcgggtgt gccccacgcg
     7021 ggccatcact gagataccgc actttgtggg ggtagttcga atcggcattg cgggcaaagt
     7081 tcaatttgtg tccggcaccc tcaaggtatc cgaggccagc agcccgccgg ttattcgtgc
     7141 cgccttacaa gctgtgccgc cgtcggactg ggtgatcctg gacgctcccc ccggcgcggc
     7201 ttgttcggtg gtagaaacgc ttcgggactg cgattatgta ttattagtaa gtgagccaac
     7261 gcccttcgga ttacacgacc ttcagatagc gttggaactg gcaaaaacat tggcggtgcc
     7321 atgtggggta gtcgtcaatc gggcttattc ctccatggcc gagctccggg aattatgtgg
     7381 acaggcgcag gtgcctgttc tgggggagat tcctgatgat ttggccattg cccgcgcata
     7441 ttcccggggc caacggatca ttgacgtggt gccgggattg catcggactt ttgaccactt
     7501 gctgcttcgg ttgctggaag agcttcaacc gaatgtactg ccgtttcgga cgcggcagga
     7561 tttagaacaa gctatctacg caaacgatcg aatggaggcg acaaatctaa acgaaccggg
     7621 ccggctgatg gcgacatgaa agacgaagct tccaagaaca tgcagagcgt gtccggaaca
     7681 atgcggaaat attggaagtc ctgtgaatcg gagttttggc agaacgtctt tcgcgttgaa
     7741 acagattatt tattgaagca tttaaagggc tgccaaaccg tcttaagtgt tggctgcggc
     7801 ccggccgtta tcgaagctgc gctgggcaag catggctacc aagttacggg ggtagatatc
     7861 gagccaggag ctttaaggga ggcttcaggc gcggtgaaaa tggtggtcgc ccgggcagaa
     7921 gatttgccct ttcccgcggc cgccttcgag gcggtaatct ttgtaacctc cctgcaattt
     7981 atcgaagatt atcgagcagc cgtggtgcag gcggcgcggg tattgcgcga gagcgggcag
     8041 cttatcgtga tgctgcttaa tcccgcatcg gcgtttttcc aacggaaact ctgccaaccg
     8101 cactcgtacg tgcactggat tcggcataca gacctcgagg aaatccaatc ggtcatcagc
     8161 gagcagttcg aggcgcagac ggagtatttc ctcggcatag acggtgcgac gttgtttgag
     8221 actcgggatc ctgcaggagc agctctattc gtcattcgag gcacaaggaa ggccgcatcc
     8281 ctatgacgga actggttgtt ctcagcggca aaggcggcac cggcaaaacc agcgtgacgg
     8341 cgtcgctagc tgctttggcc gaagcaccag tattagcaga ttgcgacgtg gatgcctcca
     8401 atctgccact ggttctggct ccacgcatcc agcaccacgg gacattctcc agcggactac
     8461 gagctcgtat tcaatccagt cgctgctccg tctgcggcca gtgcgaagag ttatgccgat
     8521 ttaatgccat ttcctactat gaatccgcca acgacgatga agccggcgca ttccgggtgg
     8581 atcccattgc atgcgaaggc tgtggtgttt gcgctcactt ctgtccagaa ggcgccttgg
     8641 aaatgattcc aagcgacggc ggtgaatggt ttttgtcgga aacccgcttg ggaccattgg
     8701 tccacgcgcg cctgcggccc ggacagggga attctggcaa attagcagcg ctcgttcgtg
     8761 aaaaagcacg cgaaacggca cgcgccaatg gtcgcgaact ggtcctgatt gatggcccgc
     8821 caggcattgg ttgcccggtc atcgccagca tgacaggagc tacgaacgtg ttggtggtta
     8881 cggaaccgac ggtcgccggc aagtacgacc tggaacgaat ccttgcgttg atccggcact
     8941 tcaacctccc tgtttcggta tgcataaaca agtatgattt gaattcggcg atggtggcac
     9001 aaatcgagcg ctacactgcg gaactcgggg caaccactgt cgggcggatt ccctatgatc
     9061 ctgccgtgac tgcagcccaa cgtcgtggca tctcggttgt ggaactcaac cagggtcagg
     9121 cggcgcctgc tatcgttgaa ttatggaaga ctctatccga aaaatgcctc aacctgacgc
     9181 ctggtcacta tcagccgatt tcggccggct aaaaagtact gacgccgtca ccatgcgaac
     9241 ttttgtagaa atcagtgtct tttgcttgca tttgcgcgct caacggaatt tccatgtcgg
     9301 caacaggaaa acctgaaggt tcccaggctg ttaccgggtt tgtttgtgaa aattgtaact
     9361 tttccccttc tgttggatca gtttaaccag acacgtattt ataaaacagt aattacaacc
     9421 atagacaaaa catgacaacc gcatccgtag cacctgaaac tacttccgcc gcccgtattc
     9481 cgttgattgg cgaaacagca ccggccttcg aggcccatac cacgcaaggc ccgattagct
     9541 ttccgaaaga ctatgccggc aagtgggtca ttctctttag ccatccggct gacttcacac
     9601 cggtttgcac cacggagttt atgacttttg ccacgatgat gccggagttt aaacaactga
     9661 actgtgagtt ggtggggctc tcaatagata gccattacgc ccatattgcg tggttgcgca
     9721 ccatcaaaga gaaaatcaaa tacaagaata tggccgatgt cgaaatcgtc ttcccggtga
     9781 tctcggatgt gaaaatggaa gtggcgaagg cttacgggat ggtgcaaccc ggggccagca
     9841 gcacggaggc tgtgcgggct gtgtttatta ttgatcccaa ggccattatt cgcgcggtgc
     9901 tgtattatcc gttgtccaat gggcgtaatt tccctgaaat caaacggttg ttgatcgctt
     9961 tgcaaaccac ggacaggcat ggttgcgcca ccccggcgga ttggcaacct ggcgacgatg
    10021 ttatcgtgcc acccccgggt tcttgtggca ccgccaaaca gcgcgtagag aagccggccc
    10081 cggatgctta tgcccttgac tggttcatga cgttcaaaaa gcttccaaaa gataactagg
    10141 cttggcggga aggaactgca cggttggcgt tgtgcaaggc ttccccgcgg cggaaaggaa
    10201 atatcatgaa tgtggccgtt ttaggagcta gcaacaagcc tgagcgctat agctttaaag
    10261 ccgtgcggat gcttcgtgag catggacaca cgccgttgcc aattcatccg gcgctggcgg
    10321 aggtggacgg tttgccggtc tttccttcgc tcgagatggt ttcgaccccg attgatacga
    10381 tcacggttta tctctcgcct cgcaaccagc agcctatcgt caaagacctg ctgagcagcg
    10441 gtgcgcgccg cgttattttc aaccccggct ctgagaaccc gagtttggct gaccaactcc
    10501 gccaatctgg caaacaggtc gtgcatgcct gcaccctggt attgttagcc accggtcagt
    10561 tcgcatcctg aaggacctgc gacggcgcac caaaacgggg tatcggggca aggacggccg
    10621 cgcgcaactg ccttaatgca atcgctggca cttccgctcg ttcttatgct ataaatagcc
    10681 gatgactcta ccaactgcac tgttactcgg ctactgcctg ctcgccctgc tggcagcctt
    10741 aattggcggc atgatgccct ctttgatcaa gctgacgcat acccgccttc aattcgccgt
    10801 cagtttggtt gctggtttga tgttgggatt gtcggtgctt ggccttcttc cgcatgccac
    10861 gcatgagttg gggtcagttc accaaagcac ttcctggctg ctggcgggat tcctggtcat
    10921 gtttctcttg cagcgatttg caccctttca ccatcatgat gtccctgaag gaagtccccc
    10981 cgagccttgt gggcatactc attcgctggc cgagagatcg gcgcgcggcc tcagttggat
    11041 tgggttggcg ttcggattat ctctccactc ggtgtttgat ggattggcga tggccgcggc
    11101 agtgaccgct gacgcgcaga caccgcgcgg tggagccttg gggcttggca cggctttagc
    11161 cgtcattcta cacaaacctt tcggggccct ggcgattacg acgctaatga ctgccagcgg
    11221 ggcaggacgc gctttaagaa actgggttaa cttggcattc gcttgtgtga ctccgttggg
    11281 agcgatgctt tttttccttg gggctgggcc gtgggcggtg gtgcatccct cctggcttgg
    11341 ttgtgccctg gctttttgtg ccggaacctt tctctgcatt gcctgcgccg acttgctgcc
    11401 ggaattgcag tttcatacgc atgatcgctt taaactttcc ctggcattaa tcatcggctt
    11461 aggtatcgct attttcatag ggcagttgaa acttccaggc cacggccaca gttcaaacca
    11521 ccccaagaca gaacacttgc atcctcccag atgagctcca ccttttttga gcggggcggc
    11581 ctttggatgc tcggccagaa tctgttgcta gtatttgtcg gagcaggcgg tttcttctat
    11641 cgcaaccatt ggcaccatgt tcttctcgcc agatgtggcg cttttctgct cctgttcgct
    11701 gcgggttgtg gcttggcggg cgcgattagc ctgggccgga atctaacgcc attccccaga
    11761 ccttcgcaga gcggtcgctt ggtgcggact ggtatttatc agtttgtacg ccatcccctc
    11821 tacgttgtcg tgttttgcgc ctcggtaggc tgggcgctgt tttgtgccag ttggcctgcg
    11881 ctattggcgg ctttggtttt agcgccggtt ctcgacgcca aagcgcggcg cgaagaacgc
    11941 tggctccaac agcggttccc cgaatatgcc agctacgaac agcgggtgcg gcgctttatt
    12001 ccctgggttt attgaaccaa atgctcggac tgactgttta cgtcggcacg gacaatgtca
    12061 tcagcacttc caaaccgcgg ggctttcgat ttttagcact taccgtcccc cggcacgctt
    12121 cgacgcaagc ctttactatc gccagtccaa ggccagttcc cccagtagcg cggctgcgcg
    12181 atggttctcc tcggaagaaa ggatcgaaaa cctggggcag ggactcttcg ggtatgcctg
    12241 ggccttcatc agcaacccct attataactg tatcatcctt gcgctcggcc gtgatgatga
    12301 tggggcccgc tggatcagca tagcgtaaag cattgcggat cagatttgct aacgcacgaa
    12361 agagcaactc cggctgagcc atgaccgtca ggccatccgg cacttgaacg tgcaaatgaa
    12421 gggttggcgc tgcctcctgt tccaaaactc gctggacagt ggcggcaaga ttaaccggct
    12481 cgagttgaac cgcttgcggt gtcagcccag ctttggagaa cgagagaagc tcgtttacca
    12541 ggcgcgacat ttgctccacc tcctcgcgca ggtcatgcat gtatgtctgc tgtttatcat
    12601 ccgttcgctg ttctagaatg ccaagagcta cctggatgcg cgccagaggg gagcacaatt
    12661 catgggcaat atcgcccaaa aaccgctttt ggcccgttac aaagcccgcc aggcgtacgg
    12721 ccatacgatt gatcgccagc cccagccggc ccagttcatc gcctcgccga tcattgaccc
    12781 gcgtctcaaa ccaaccctcg gaaatttgct cggtagcgcg agtcatctgg gaaagcgacc
    12841 gggttatgcc tctgatcaaa ggcaaccaaa acagcaccga aaagactaat gctcccactc
    12901 ccaccatcac ccacggcgtg gggtcaaaaa acaaacctcc agcactgagc gagcttgaat
    12961 tcagcagaag agttagtggc cagggacggc tggtgccagg ctcagccaga ggtaaacgaa
    13021 tgccaatcca atatcccgtt ggattgctgg tgcgcacaac aaaccgcgcc tccggcctga
    13081 cgtcggattc ttctcgcgtc caacgaggcc ctcgcccagg cggcgggccg cgcctcaccc
    13141 ccgcgcctgg attgggccgt tcggcgacct tagccgccac ttcggttggc aggtccatgg
    13201 ttgcaccggc cacttgagaa ccgtccggac gaaagaggaa gaattgcact tgataggctt
    13261 tgccgaaacg ctggagaact tcgtcccagg aactaacgga cttggctttg agttcgtcgg
    13321 taattacatc gctgaccgct tgcacgcgtc cgcccgcgcg tcccgccagc aaggaatcca
    13381 atccaaactg aaattgtaat ctgacaacga gatgtatcgc cgcacctaga accgccaggt
    13441 tcaggagcag ccaaatgaga atttttggca acagcggaaa acggaggtgc aaaagcataa
    13501 atacgcgcca ctaggcgtca ggattgatca gcatgtagcc cgcacttcgc accgtgcgaa
    13561 tgtagcgcgg gtcccgggca tcatcgccca gcttgcggcg cagcgtcgag acgtgcacgt
    13621 caattgagcg gtcgaatacg tcgtagtgcc gctcccggat ttcctccagc agttgctcgc
    13681 gcgacttgac ccgcccccgc gcgcgcgcca gggccgccag caaatcaaac tcgacggggg
    13741 tgagcgtcag cggctggttc gccagcagcg cgacgcgtga ggcgtggatc acgcgcagtg
    13801 ctccgacctg gatttcgggt tcgttttgtg gttgcgcgct cactactgtg cgcgaggagc
    13861 gtcgaaccac cgcgcgaagt cgggcgagca gttcacgcgt cgagaaagtc ttgggcaagt
    13921 aatcgtccgc gccaatctcc agccctacaa tccggtcggg ttcctcgccc cgcgcagtca
    13981 gcatgagcac gggaacgtcg gacgtgcgcc gcagtagccg gagcacctcg aaaccatcca
    14041 tggcgggcaa catgacatcc aggatgatag cctggaactg ccccgtttgc gccttctcca
    14101 gcccttccgg ccccgtatgc gccgcttcga cttcaaaacc cattggctcc aggtaatccc
    14161 gaatcagccg acagagtttc ttgtcgtcgt caataatcag caaccggggc tttatctccc
    14221 cggcctgcgg gcgcctggct ccttccgtta gcatctttgg ttccacaggt tatcttagcg
    14281 tgcgtgcgct gattgacgcc aacttttaca ataccttaac aattttctcc cgcggcacac
    14341 ataaaagctt aacattgcgg gagtctattt tctatagggg ccagtagttt gaacggcgtg
    14401 gccgactcaa gcctggcgca aacataagaa catagaaaac aatagaaaac gtatgaaaca
    14461 aacaataaca ctagttatcg ggctggtcct tgccggcggg gtactaaccg ttggcgctca
    14521 acctggccag ggacgaggcg caggagccca acaccgacgc ggggcgcagc aattcccgcc
    14581 tccgattgca gtcaccttcg acattgactt ggacggtgtg ctgtcggcgg cggaaataca
    14641 gcaggctccg agtcgtttgt taaagctcga ccagaatggg aacggacaac ttgaacagca
    14701 agagttgtgc tctggggggg gaggcccagg cgggcagacc tgcatgtggg gggggcaggg
    14761 catagggcgc cgcgccaatg gcaacggtca aaacgcggta ttgcttgccc ttttcgacag
    14821 caataaagat ggactccttg attccgcgga gatcaatggt gcctcggcgg ttctcaaggc
    14881 cctggataag aacaacgatg ggcaggtaac tgccgcggaa atccgcccat tgccggggcg
    14941 agggcaaggc cgtccgtggg ctcaaaagta atcagcagta ggctcgggat acaccttaag
    15001 tttaggccga gggcaaccgg gcgcaaagcc aagccttgcg ctccggttgt tgccgtcgtt
    15061 gactgaaagg aacgcccctc tgaagcaagc ccgagcatac tcttctccga agcgtcagaa
    15121 tcggtgaaca agcttttcga ccaggaacgt actctcgcgc atcgcctgct cgctgaaagg
    15181 gccaaaccat tcggcgatct ggcgaaagcg ggcgataaat tcagttttgt cgcctcgttc
    15241 gacaaggcta aggttctttt gaagggattc gagataatct tttagcagtg cctgacgctc
    15301 cggtgtggcg aagataattt cggcataaag ttgaggatcc tgggcgaaga ggcggccgac
    15361 catcgccaat tccaagcggt aaatcgggct ggaaagttcc agggtgcgca acacgggcac
    15421 ccctcgcgag cataaaaatt gaccaaaggt aaacgtagca aagtggcgca aagcctggac
    15481 gatccccata atctcatcgt gctcggaggc aggtgtctca accaatacgt tgccccatag
    15541 agtcagttga tccagaaacc attggcattt gctcaaggaa cgccccgggc tgaagacaac
    15601 gatttgtttg tccatggtga ctgtcgccgg gccaaagagg gggtgtaagc cgacgaccgg
    15661 gccggcatgt cctcggagca tagcttccag gggtttacgc ttaaggctcg taatatcagt
    15721 cagcacgcaa gcgggcggga ggtatttgcc tatctgcagc gcaatggacg gcgtgatttc
    15781 tataggcact gccagcaggc acagatcaat cccggttgtc aacgagcgta ctttgggcca
    15841 atcgtcgcga tccagaacgc ggacctcgta gtctgactga cgaagccagg tcgagaaaaa
    15901 gcgtcccatg ttgccaagtc cgccaacaat gagaattttg gcgcccggcc gaacgctgcg
    15961 gcggctcaag gtactgagtt gtccaacgcg ggaatgccgg agcatcgttc ggaatagatc
    16021 ttccacgtag tcagggtcta atccagcctg ggttgcctgg gcgcggcgtg tggaaatcag
    16081 gttttcttcg cgtgccgggt gaaaagtggg cagatcatgc cgttttttga tgtcggtaac
    16141 ctgttggatt atccgacctc gttggaccag caggtccact aattgctgat ctatcccgtc
    16201 tatctgctcg cgcaaggaat ccaaagcaga ctttgcgccc tgcgcggaac cggaaggcgt
    16261 cggcccgggc aggcatggtt tggaacgcgg cttagacatg gctagcctcc aagtgatgtc
    16321 ctggaataat cattgcgcat aaggtagcta atgttcgggc gcttggcaag gtggacggtt
    16381 ggacgcgaaa aaaggggaga acactttcgg attccttgcg ttcagatgcg ccggatggtt
    16441 agtgcttgcg gctttcagac ctccgtccca gtgcggtttt tcgtgacgga agcgggcttt
    16501 ggtattaggc tcgaggcatg ttgagtcaac gttggcggcc ggtatgttgg gccggggtaa
    16561 ccatcggcgt aatttggctg ttggcgctgg ccggataccg ccttgttgga aatactaaaa
    16621 tgaccgcggc gaaggtgaaa gagtacgccg agtccgtgga tattagccaa ctgaaggatg
    16681 atgcgcgggc gaaggcaatt caaggattgg ctgaccggct gaatgcgctg tcgctggaag
    16741 agcggcgcaa ggcgcggctg gagcaggagg catggaactg gttcgaccag atgacggagg
    16801 cggaacgcgc gggttttatt gaagcaacca tgccgaccgg tttcaaacaa atgctggtgt
    16861 cgttcgagca attaccggat gacaaacggc gcaaagcagt agatgacgcc ttgcggcggc
    16921 tcaaagaggc gcgcgcccgg gccgggcttt ccctcggacg ccgcgggact aactccccgc
    16981 ccgtgctgag cgacgagctc gaggcccagg tgcgaaagat cggacttaag accttttaca
    17041 gcgaaagttc agcgcagacc aaggccgaac tggctccggt gcttgaagag ttgcagcgcg
    17101 ttatggaaag cggacggcca ttccggggga gatgagcgta ttccgacgaa acctttcgcc
    17161 catgaattcg gctgttgagc aacgtactgg ccccggttca gccaggggaa cccgatccag
    17221 acaggaaaca atccggggct ggtttgcggg tgccttcacc ctgatcgaat tgctggtcgt
    17281 gatcgcaatt atcgccatcc tggccgccct attattgccg gctcttagcc gggcaaagga
    17341 atcgggacag gctacggtct gcctgagtaa cctgcgccaa attgggctgg cgttgcaaat
    17401 atatgtgagc gacaaccaca atcggctgcc gaccatgcgg gaccgatcgt tgaccacgct
    17461 gaccaacgaa ttgcccagcc cggatctggt gctttcgaac tacgtgggga atttgaatgt
    17521 atttaagtgc ccctcggaca aatggcccgg agatacggca aaactcttac ccggggcggg
    17581 cgcgaccttc tacgagcaaa ccggttccag ttttgcctgg aacagcctgc tcaatggccg
    17641 ggacgcagag catctggttg cgtttggcct gaaatttgat ccgcaccaga tgccgttgat
    17701 gtacgacaag gagaaattcc atatcgcccg tggggctgcc aaagcgcaga actggctcta
    17761 cgcggatggc catatcaaga acttgctatt gttcgaaggc accattcgaa aatacccgtg
    17821 agcgaaccga tgattcagct caagcgcctc aacaagcaat ttggcgcgcg ccaggcggtg
    17881 aataatttga cgctcgccgt gccggccggc gaaatttacg ggctgctggg ccacaacggc
    17941 gcaggcaaga gcacggcgat cgggatgatg ctcgggcagg tctggcccac cagcggagaa
    18001 gtgctgattt gcggttatga tgtctccgcg catcgccggc aagccttgca gaaagtgggg
    18061 gcgattttcg aaagcccggc tttttacgac tatttgagcg ccgtgcgaaa tctggaaatt
    18121 ctaagctcct acaccgccct ggtatcgcca cgacgcattc aggaagtgat cgaatgggtg
    18181 gggcttagcg ggcgggaacg ctctctggtg agaacctact cacacggcat gagagcccga
    18241 ctggccctgg cccaggcgct attgccgcaa cccgaattgc tcattctcga cgaacccagc
    18301 gatggcctgg acccggaagg cattcatgag atgcggcaga caatcctgcg gttgcgccag
    18361 gaactagggc tgacaattct gctttcctct catttcctcc atgaagtgga acaactttgc
    18421 acgcgaattg ccgtcttaaa ccagggctgg atggtttttg aaggcgcact ggcggccacc
    18481 aaaacaaacg agatctgggt gcggcttaaa acgaaggatt tcgggctggc ggtaaaggaa
    18541 ctgctgcagg cgcggatgat tacgcgggat cgcgatgaat gtatgatcgc gttgagtccc
    18601 ggcgtggaga ccgaccagat tgtgcggcga ctggtagagc gcgccattcc ggtttatgaa
    18661 atagtcccgg ccgagcagac tcttgaagat ttctacctct cgctgatgga gcgacaacga
    18721 aaggcgcagt gaacatgttt ggccttcatt tgcgcaacga actctggaag ctctttggaa
    18781 agaagcgcac gtatattggg tttggcgctt ttctactggc gcaaaacgcc atgctgttgg
    18841 cgttcaaatt tacccgctgg caatctgaaa tggagcggtt gctggaaggc aacggctacc
    18901 tggccctgga atacgtatcg gcgttgacgg tagctttgat catgctgatt ccacaaattg
    18961 cgctgctgat gcctctctac acggccctgg tcggaggcga tctggtagcc aaggaggtgg
    19021 aagatgggac gttgcgcatg atcctgtcgc gccctatttc ccggtttcgg ctattactgg
    19081 tgaaatggct ggcagggggt attttttcgg cggcgctggt tgtgatgctc ggcgtcacgg
    19141 cgctcggttt tgcccgccta tggtttccct ggcagggaat gttcgtgttc attcccgggc
    19201 agattttcaa cgtattgtcc ccggccgacg gtttgcggtt ctatgccttc tcgcatctat
    19261 ttatgaccct taatgcctgc acggtgctgg gaatcgccct gatgttttcg agctttaaca
    19321 tcaagcccgc tgccgccacc atcctggcct tatcgctctt gttcgctaac ctggtaatgg
    19381 aaggcatccc gttttttgag cgatatcacc agtacttgct gacctttcat ttccatgcct
    19441 ggcgtctcgt gttttcacag ccaatgccct gggcgcaaat cgcgcaatcg gtctgcgttt
    19501 tgctcgccgt taacctcacc acctttctta tagccgcggc tgcctttcaa gcgcgcgaca
    19561 tcaaatcgta ggcccttttt aggcgggctc atacccccgc agactcaacc acgccgaaac
    19621 gccgatttaa ccccatatat cgcggcgatc ccagaagctc cccaactaat ggtgttttta
    19681 caaggggatt aattagaaat tgctattgtg atcctggctc atcttttcta tactttcccc
    19741 gtgaaaatcg aaagccaaaa tggcctctgg aaacggtagt tatgcctgtg gaaaataccg
    19801 ttgaaccgac cgggaaattg gccgccattt catccgcgcc cgagaagtat gatgcgtcca
    19861 agatagataa gctggaaggg ctggaggcgg tgcgcaaacg cccgggaatg tatatcggcg
    19921 acccggacga gcgcggcttg catcacagtg tttttgaggt gctcgacaac tccattgacg
    19981 aacacctggc cggattctgc agcaagatcg aggttgccgt tcatgtagat ggctccgtct
    20041 cgattcgcga taacgggcga ggcatcccgg tggacatgca ccccaaatgg aatatgccgg
    20101 cggtcgagtt ggtgctgacc aacctgcatg cgggaggcaa gttcgggcag ggcgcttata
    20161 aatattccgg gggattgcat ggcgtcggcg cgaaatgcgt caacgcgctt tccgagtggt
    20221 tcaaggtgga agtgtcgcgc gacgggaagg tttattccat ggagttctcg cgcggcgtca
    20281 ccacgcaaaa actgaccgtg gttggcaagt ctcgcggcac gggcacgctc atcactttca
    20341 agcccgaccc gctcattttc acgatcacga ccgagtttaa gttcgacgtc ctggcaaacc
    20401 gcttgcggga actggcgttt ttgaacccgg gagtggagat cgtgctgacg gacgagcggg
    20461 acgagaagac cgagaccttc ctgtacaagg acggcattga gcaattcgtc aagcaactgg
    20521 gccgaaccaa gcaggtgctt caccccaaac cgatcgtcat cagccggcag aaggaagaag
    20581 tgttcgtgga ttgcgtaatg caatacaccg acagctataa tgaccagatc ttatgcttcg
    20641 ccaactcgat cgccaacccg gacggcggga cgcaccttac cgggttccgg tcggccctga
    20701 cgcgcgccat caatcaatac gccaagcaaa gcaatttatt gaaagaaaag gacccatcca
    20761 tctcagggga tgacgtgcgg gaaggcctgg tgtgcgtgtt gagcgtgaag ctgcccaacc
    20821 cgcgcttcga gtcgcagacc aaggtcaaac ttgtcaatac cgagattgat gggctggtat
    20881 cctccgtcgt ctatgaaggg ctgatgacgc actttgacgc cactcccgcg atggccaaga
    20941 aggtggtgga aaaagccttg ctggcggcac gagcgcgcga agcagcccgc aaagcccgcg
    21001 aaacggtgcg caagggcgcc ctgacgggcg gcggcctgcc tggcaagctg gccgactgct
    21061 cggaccgcga tcccgtcaat acggagctgt atattgtcga aggcgattcc gccggcgggt
    21121 cggccaagca ggggcgcgac cggaagttcc aggcaatcct gccgattcgc ggcaagatca
    21181 tcaatgtgga gaaggcgcgc ctcgacaagg tattgcaaaa caacgaaatc cgcaccctga
    21241 tcactgccgt gggcaccggg attggcgacg gggaagggga gggggcgttt aacctggagg
    21301 ggctccgcta ccacaaaatt atcattatga ccgatgcgga cgtggacggt tcgcatattc
    21361 gcaccttgct gctgaccttt ttctatcggc aaatgccgga attgattcgg ggcgggtttg
    21421 tctatatcgc gcagccgccg ctctaccaga tctcgcgcaa aaaacgcacg gaatatgtgg
    21481 atgacgacgc ccggctgaac aaaatcctga tcgaactggg cacggacgaa gtgcgcctgc
    21541 gcaacctggc cgatggcaaa gagttgaccg agaagcagct cgcggaaatc ctggaattgc
    21601 tggaatcgct ggacaaatac gccaatgcgt tgcgccgtca cggcggtgac tttgcggact
    21661 acgtcgagca gcggcatccc caaacccatg agctgccgcg gcacctggtg aaagtccgca
    21721 atggcaacga ggagaccgtt cattacttcc attccgagga ggaactggag gaatttggtt
    21781 cggccaaccc ggatttacgc ctgttcggag aagaagactc cgacaccagc ctggtggaaa
    21841 agaacaagaa cgggcatgcg cggcgcgcgc ggcacgtcga actgcatgaa agcaaggccg
    21901 tggcggaact gctggccagg ctgggccaga agggcttaag cctcgaacat tactcggcgc
    21961 aggacaagcc gctcttcgaa ctcatcgaag gcgagggcga caaggaagaa atcaaacccc
    22021 tgttctccat cccggagatt ctgtcccgcg tcaaggaagt cggccggcgc ggactgtcca
    22081 tcaagcgctt caagggcctg ggcgaaatga accccaagga actgtttgag acaaccatga
    22141 atccggcccg ccgcaaactg ctgcgcattg atttgacgga cgccgtcgag gccgaagaga
    22201 tgttcgtcaa attaatgggc gatgaggtcg aaccgcgccg ccaattcatc gaagataacg
    22261 cgctgaacgt gcgaaacctg gatatttgac ccgtgcccgc cgccttgcgc cacctccgga
    22321 ttcacgccct ttaattttcg gcatgccaga cgatacccct tccaacgatc cgcgccccgc
    22381 tctcacgccg gcgtacgccg cccatgagaa gatcaacaag atcaacgtgg cggaggagat
    22441 caaaaactcc ttcctggatt attcgatgtc cgtgatcatc tcgcgggcgc tgccagatgt
    22501 ccgcgacggc ctgaagccgt cgcaacgccg cattctctat gcgatgcacg acctgggcgt
    22561 gatgcccaat cgcaagcatc tcaaatgcgc caagatcgtg ggcgaaacga tgggcaacta
    22621 tcacccgcac ggcgacctgg cgatttatcc gacgctggtg cacatggcgc agccctgggc
    22681 catgcgcgag cggctggtgg aagggcaggg gaacttcggc tccgtggagg gcgacccgcc
    22741 ggcggcgatg cgctacaccg aagcgcggct ggcgccgctg ggagcggtgc tgatggagga
    22801 catggacaag gatacggtgg acttcgtgcc caattacgat gagacgcgca ccgagccaac
    22861 cgttttcccc gccgcgttcc ccaatctgct ggtcaacggc ggcacgggca tcgccgtggg
    22921 catggcgacc aatatgccgc cgcacaacct gggcgagatc gtggacggca tttgcgccca
    22981 gattgacaaa ccggacatta cgctccggga gttgatgcag cacgtcaaag ggccggattt
    23041 cccgacgggc tgcatgattt gcgggctgga aggcatcaaa aactatttca cggatgggcg
    23101 cggcagcctg aaggtgcgcg gcaaagtggg gctcgaggaa atcaaagggg ggcgcgaaca
    23161 gatcattatc accgaaatcc ccttcaacgt gaaccgggcg gtgctggtcg aacgcatagc
    23221 cacactggtc aacgagaaaa tcatcaccga catcaccggc gtgcgcgacg agtcggatga
    23281 aaacacgcgg atcgtcattg aaatcaagcg cgacgcggtg cccaaggtgg tcatcaacaa
    23341 tctctaccag tacaccgcgc tggaaagcag cttttcggtc aacgcgctgg cgatagatca
    23401 tggccggccc aaaaccctgg ggctgaaaga cctgattggc tgctacatcg agcaccggcg
    23461 cgaggtggtg attcggcgca cccgcttcga gttgcgccag gccgaggaac gggcggaaat
    23521 cctggaaggc tatctgatcg cgctgtccaa cctggacgag tttatccgca tcattcgcca
    23581 ttcccacacc cgggaggagg ctaaaatcaa gctgctggct tttgacttca cccgggcgca
    23641 ggtggagacg cttggcatcc tgattcgcag cgaggcgcgg ctgctcaacg ggcgctactc
    23701 gttcagcgaa gcgcaggcca acgccatcct ggaattgcgc ctctaccagt tgacgggcct
    23761 ggaaattgac aaggtccggg ccgagtatcg cgccttgatc gcgcggatca aggacctgct
    23821 ggatatcctc gcgaaggagg aaagggtgct gggcatcatc aaaacggagt tgcaagccat
    23881 caaggcgaag tacgccacgc cgcgggtaac ggaactggtg ccggacgagg gggagattgc
    23941 gattgaagac ctgattgcga acgagggcgt catcatcacc ctcacgcata cggggctgat
    24001 caagcggacg aacatcagtt cctaccgcgc ccagcgccgg ggcggcaaag gcgtgatcgg
    24061 catgacaacc cgcgaggggc ccacggagga ggacgtggat ttcatcgagc acctgttcac
    24121 cgccagcacc catgattacc tgatgttctt tacgaacacc gggcgcgtgt acgccgaacg
    24181 cgtgcatgag attcccgaca tggggcgggc cgccaaaggc cgcagcatcg ccaacctgct
    24241 ggaactgaag gccggcgaga ccatcgccgc gttgattcgc attgaagccc gaaccggccc
    24301 aaacaaggaa gacatcacct ggcagcagcc ggggttcctg tttttcgcca cgcagcaagg
    24361 cacggtgaag aagaccccgc tcaccgactt cgccaacgtg cgcaaagacg gaatcatcgc
    24421 cattcgcatc gagccggggg acgcgctgat tgacgtcaag ctgacttccg ggcaggacca
    24481 ggtggtcctg atcacgcgcg atggcatgag cattcgcttt gccgaggaag acgtgcggcc
    24541 catggggcgc ccggcggcgg gcgtgcgcgg catcaacctg gagaaagccg atgccgtcgt
    24601 ggccctcgcg ctggcggttc ctgacgcgac cttgctggtg gccggcgaaa acggcatcgg
    24661 caagcgaacc ccctttgacc tggcccggga ggatggaacg accgagccgg tctatcgcct
    24721 ccagtcgcgg ggcggcaagg gcatcattac gatgaaagcc aatgagaaga ccggcgctgt
    24781 ggtgggcgcc ctgaccgttc aggacggcga cgaagttatg ctgataacgg caggcgggca
    24841 actggtgcgc acctacgtga aggacattcg ggagacaggc cggaatgcgc agggcgttcg
    24901 gctgattaac ctggccgaag gcgatcaact gcaggctatc gcgccagtga tcagcgagca
    24961 gcaggagaac ggtgcggaag aatagccggg ggcagcgccg cccggcggca accgcggtcg
    25021 cgggaaacag ccgccgccgc gaggcggggc gcgtcgggaa gactcagggc gacaggcgct
    25081 cgatccgcca ggtgttatcc ggctgccggg tatagcgaaa ccgatcgtgg aggcggttgg
    25141 ggcggccctg ccagaactcg atccgcgcgg gccaaagccg gtagcccccc cagaacggag
    25201 ggcggggcac ttccttcccg gcgtagcggg cttcgatctg ccgccacttc tcctccaggg
    25261 ctgcccgatc cggaatcacg gtgctttgcg gcgaggccca ggccgcctcc tggctgcccc
    25321 gtggccgcga atggaaatag gcatcggact ccgccggcgg cagttggctg atttcgcccg
    25381 cgatgcacac ttgccgctcc tggttcgacc aataaaacac caacgccgcc cggggatttc
    25441 cagccaactc ctgccctttg cggctttcgt aattggtgaa gaatacaaag ccgcgctcat
    25501 ccacaccctt gagcagcacc acccgcgccg acggatgccc atgcgagtcc accgtcgcca
    25561 gcgtcatcgc cgtcaggtcg agcggctcag cgccgctggc caggagcagt ttcttgtaga
    25621 gcttgatgcc aaacttccgc agccgcccgc tggcgcggac tcccgccgcc tgctcaaacc
    25681 acttcttgaa ctgggcgatg ggatccgcct ccaaatcccg ccgccgcaga ccggtcaggt
    25741 tgtattcacg ccgcaagtcc gcaatagcca taacccaaaa tcaaaacccc gcccgttatc
    25801 ccgccaggcg ggagccgggg agtgagggcg aatcgcgcat acaggcgtcg ccgggctacg
    25861 gctgcgcggc cgaactggca ggcatcatcc ccgtcttgcg cgcataattg aagatgcctc
    25921 cggcatccac cacgggccgc acatccccca gggacttaaa cggatagacc ttgcccgaat
    25981 ccttgctggt gacggtgccg gcatccagat ccaccgtcac gacctcgcct gttttgaggg
    26041 cgtcgcagag ccgttcgacg gtttcgcagg gatacaactc gcccgtggca acggcattgc
    26101 ggaagaaaat gcgcgcaaag ctttccgcca gaacgacctt gcagcccgcg gaacccaacg
    26161 caatcggcgc atgttcccgc gagctgccgc agccaaaatt gcgcccggcc accacgatcg
    26221 gatattggct gtcgagctgg ccttcgtcca cataacgctt cggatacgcc gattccggca
    26281 atcccgcgaa ggcgtaagcc cccagctttt cgtactcctg cggaatcgtc ggcacgagat
    26341 tcaagtattg cgccgggata atctgatccg tgtctatatt atcccgcacc acaaagactg
    26401 gtccggcaaa aacagattgc atgagcccaa cttggacgac ggccagccca ttttcaatca
    26461 gatattgaca ggaatctact tttcgaacgc ctgcctggat aactcgtccg cccaaaggaa
    26521 cggctaaaac tggacgcaac cacgcacccc aaacccgaat aaagccgaaa gccaactcca
    26581 accaaatcct gccgaactgt ccaattattg gacacttgcc tgctttcggc ctcaatagcc
    26641 atttccaatg ccagatttcg cctaacctgc ataatttcac ggacatccat agataccagc
    26701 tggccgtgta caaggggtgt acaaggggag tacaacggat gctcagcaga ggaacaatga
    26761 aaaatccgtt gtgcatccgg tgagcatccc ttgtacaccc cttgtacacg gcgctttgag
    26821 atggtatcga gagggtgggg gcagggaagt gccgacgaga gcgtgccagg gcaggggacg
    26881 gtatggcctt aaaaactatc ggaaacgacg aaatttacag ggacaacgga ttgacattgc
    26941 tttcccggat ggttttcggc atccttgcct gtgaaaccta tgaagcagaa gggtcgaatc
    27001 gctgtcgcgc cactggctac tggccaggtt tggcggatgg cggattcaca tcttcatgtt
    27061 gacctcgtag gcaagcacct ggtccattac aagctcatca agcgcgacgc caagcgcacc
    27121 cccatgtcgc tttccaacaa gaagactgtg gagaaatacc tcaggaccaa caaagccgtt
    27181 ttggtgcagg gataaacgca tttgccccaa aaacggggtg gcctggcaga aaagagacag
    27241 agcgtgctgg cggcgggctg cgcacctcaa aaccgagtcg ttcccccgag gagtccgtgc
    27301 cttcgccccc caaaggctca cccccaatat acgacactct ctttaagctc cggttaaaga
    27361 ctccgcccgc tgggaacacc atgccctgcg gacacaagcc tgtattcagc cgccccggag
    27421 attttatccc ggcttgtctt ttcgccaact ccggcttaac agggaaacat gaaccgcaga
    27481 caattcatcc aagccggtgc ggcgggattg gcgttggcgt ccctcggcgg ctatgccgct
    27541 gaattcgcag accagaagaa gcgtgttggc ctcatcggct gcggttggta cggcaagagt
    27601 gatttgctcc gcctgattca ggttgcgccg gtcgaggtgg tctcgctatg cgacgtggat
    27661 aagcagatgc tcgccggggc ggcggagatc gtcgccgcgc gccaggcctc caggaaggcg
    27721 ccccgcacct atcatgacta ccgggagatg ctccgcgaaa aggacctgga gattgtcctg
    27781 atcgccacgc cggaccattg gcacgccctg ccgatgatcg ccgcggtgga agccggcgcg
    27841 gatgtttatg tgcagaagcc aatcagcgtg gatgtggtcg aggggcaggc catgctggcc
    27901 gccgcgcgca agcataaacg cgtcgtgcag gtcggcaccc agcgccgcag caccccgcat
    27961 ctcatcgaag cccgggacgc cattctgcgc gagggaaagc ttggcaaaat cgggctggtg
    28021 gagatttaca gctattacgg cggcggcgcc ggaacggagc ccgaggacag tgcgccgccc
    28081 gattaccttg actgggagat gtggaccggc ccggcgcctc tgcgtcccta caaccgcatg
    28141 aagcacccgc gcggctggcg atcgttcatg gaatatggca acggcaccgt cggcgatctc
    28201 tgcatccaca tgctcgatgc ggcgcgctgg atgctgggtc ttggcgcgcc gcgaacggtc
    28261 agctccgccg gtggcatttt cgtgcgccag ggccggaagg ccaacatcgc cgacacccaa
    28321 accgcgatct tcgacttcgg cgatttgcag gtggtctggc agcatcgccg ctggggccaa
    28381 tcacccgacc cgcgctatcc ctggggcatt acgttctggg gagagaaggg acatctcaag
    28441 gccagcatta acagctatac ctttgtgccc gccgataacc gtccccctct gcaccgcgaa
    28501 gtgacgatgg agttggagca ataccccgag gaccgggagg aaaaggaact ggaaaaacaa
    28561 gtcgcgcccg cgatacgggg ccacatgaaa gactttctgg ccgccattgc cacgcgtggc
    28621 aagcccgtgg ccgatatcga gcaggggtat atttccacga cggcctgcat cctggccaac
    28681 ctgtccatga agctgggccg cagcctggcg tgggacgccg cacgggggcg cgtggtcggt
    28741 gatgaggaag ccaacggtct cctggcacgg ccctaccgcg cgccctggga gcatcccgag
    28801 ccgttgaaat tcagttagtc ccggcccgcc gggtgggcca ccgcttccgg cgccgccgcc
    28861 gcgcctacac cttcgccggc acgacaaacg gtgggcgata agggcgcgtc agcatttcgt
    28921 tggccgcccg gtttcccgca aagcgttctt tccgggggtc aaacttgagc ggcatcccca
    28981 accacacctt gtccttcgcc agatccacgc cgttggcatc cagatgcccg gccatgcggt
    29041 caaaggcctc ggccaggctc cggttatccg caagcgcctg ccggatttct cccggcgcct
    29101 tttgcgcccc cagccgatag gagatattcc cggtatgaac caggcccgcc gacaaatggc
    29161 cttccaggat ctccgcgttg agcgcttgga cgttgcggct ccggacagcg gcgatccagt
    29221 tgccatgatg gccgcccgat tccgccgtca accccaccgg agtcttcacc gaatcggccg
    29281 cggccttctt tcggcccgtg aagcgcgtga tttccttgcc gtcgcggtca tagatgatcg
    29341 cttcggtata gctggggact accacgctgc catgctcgca ctcgatgacg ttgccgatgc
    29401 tcacgccccg gtagttgtcc atcttccagc cctggctttg cgccgcctgg tcgtgcggca
    29461 agccgcgcac ttcaaagagc agcggggttt tgtatccgtg atagacaatg agagtgttgg
    29521 gggtttcggc ggaatcctgg tagcccaggc gcccgccaat ggcttgcgtg aagggcgcaa
    29581 tggtcttttc gcccagaaac cagcgggcaa tatccaactg gtgcgggccc tggttcccat
    29641 aatcgccgtt gccgtagttc caaaaccaat gccagtcgta gtgaatcgtc ccattgccgg
    29701 ttttgcggcg cggtggttcg tttggggccg ggccacacca gagatcatac tcgatgtaag
    29761 cgggcaccgg ctgcggccca tccaccgttc cgatactcct gcgggccttg taacaaaagc
    29821 cgcgcgccag cagaatccgg cccaggcgcc cctcgcgcac atactgcacc gcctcctgca
    29881 gccctcctga cgaacggcac tgcgtgccgc attggacgat gcgcttatat ttgcgggccg
    29941 cttcaaccat tttgcggccc tcgaaaaggt tgtggctggc cggtttctcg acgtaaacat
    30001 ccttgcccgc ctggcaggcc cacactgttg ccagcgcgtg ccaatggttc ggcgtcgcga
    30061 tggaaaccgc gtccacatcc ttgttgtcga gcacccggcg cagatcggtg caggcctgga
    30121 cgctgctgcc cagttcccgg gccttggctt ccagcacctg gcggtccacg tcgcaaatag
    30181 ccaccacccg cacgccgctg agcctgttca gggccttgat gtggccggcg ccctggccgc
    30241 gaaagccaat caccgcaacg cggatgtcgt cattggcccc gacagcccgg ctccaggtgc
    30301 gagcgtccag actgaaagcg gcggcgccga gggcaacggt tttgaggaaa tcacgacgat
    30361 tcaggttcat aatagtattg tattgtcttt ggattcagta ggggaaggcg gaggtcaatt
    30421 caactccttt ttccttaact ggcagaaaac cgcgcactgt ctgccagccg ccaggcattc
    30481 cgcgagaccc tttttgcgac gctgagaggg cccctgtagc atcgggcact tcagctccac
    30541 agcagccgat tcggcaagga ggagcacttt cgcattcgct tgacggagat gggctttcca
    30601 tcatacgata gatctatata aacagttaga gcccaatccc gcgcctctcc atccaggctc
    30661 tgcgccagcg caaagctcac cgcctgcgct ccctcaagct gccgccccgc ctcatttaca
    30721 cctccctgct ggaacggttc ctgacctgcg ggcgccccca ctgctcctgt catcaaggcc
    30781 acaaacacgg tcccttcttc tacctcaacc gctgcctgtc caaaggtcgc atgagcagcc
    30841 tgctgctcaa atccgacgct cagatcaccc aagcccgcca agcggtccag gcctaccgcc
    30901 aggtgctcgc agctctcgac caactctctt ggatcaatct ggaactgctg cggcgcgatg
    30961 agcccttgtt cgggaaacca accccagtat gagactgcgt cagttcgccg cctatgttcg
    31021 caaagtcttt cgcctccacc ggctggccgg ccgcatcagc ggctcccgtt gtgaccccga
    31081 catccccctc ggccatctgg ttaagtttgg acttcggtgc cgtgttcacc ccccc
//
LOCUS       DAOPQG010000034        30600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1941_length_30600_cov_15.931445, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000034 DAOPQG010000000
VERSION     DAOPQG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 30600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30600
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_30600_cov_15.931445"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(707..1693)
                     /locus_tag="PKI07_05605"
     CDS             complement(707..1693)
                     /locus_tag="PKI07_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOC50563.1"
                     /translation="MAETSSKTKYYVGVDLGGTKILSGVFRNSLECVGTAKLSTKSQR
                     GVPAVIERVARCVREALDEADLPLKQVAGVGVGAPGAVNFAEGRVIFAPNMEGWKDVP
                     LKKRLEKQLGLPVFIENDCNIAALGVHVAELKAKPRSLIGIFVGTGIGGGLIINGNLY
                     AGFNHTAGEIGHMVLDVNGPKCGCGNKGCFEALASRTAIFQQIKAGVKDGAKTLLTDM
                     LGDHLDDLRSGDLRKAIRRGDKFVAHIVEDAAEYIGIGVANLVNLFSPEVVVLGGGVM
                     EALADEMMSIIVKTARDYAMPGTMKGVEIIASKLGDAAGITGAAVLARRETR"
     gene            complement(1703..2407)
                     /gene="tmk"
                     /locus_tag="PKI07_05610"
     CDS             complement(1703..2407)
                     /gene="tmk"
                     /locus_tag="PKI07_05610"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOC50564.1"
                     /translation="MARRRGARRFYGHGLPGVDPERLAGTLIVIEGADGSGRSTQLDL
                     LVEWLENRGHATAQVGLKRSTLVSEELQRAQQGNILSRTTLSLFYATDFADQLENSIL
                     PALKAGFVVLADRYIYTLMARDMVRGMDEAWLRNLYGLALEPDVVFYLHVPPAELVQR
                     TFAKNATLDYWESGMDLGLSRDMFDSFMKYQTAMQKTFRSLQASYGFTIVDGLRPAEV
                     INAELRKKIGTVLAGK"
     gene            complement(2431..3174)
                     /locus_tag="PKI07_05615"
     CDS             complement(2431..3174)
                     /locus_tag="PKI07_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HOC50565.1"
                     /translation="MKTFAELDFPGRLIAVEGLDGSGKSTQIYLLKRWLESQGLKVYF
                     SEWNSSELVKSATSKGKKRELLTPTTFSLIHATDFADRYERHLVPLLRAGYLVLCDRY
                     IFTAFGRDVVRGCPPEWVRGIYSFAALPDLTFFFKADLEVSLKRILDGRAQLKYFEAG
                     MDLRLSPDPHESFRIFQGRILEQYLALSTEFNFLVVNANEPVENQQAVVRKLVAQRVN
                     LARFRCAERPARKTPPPPEKAVEPEETES"
     gene            3405..3479
                     /locus_tag="PKI07_05620"
     tRNA            3405..3479
                     /locus_tag="PKI07_05620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3437..3439,aa:Val,seq:cac)
     gene            complement(3587..5026)
                     /locus_tag="PKI07_05625"
     CDS             complement(3587..5026)
                     /locus_tag="PKI07_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC50566.1"
                     /translation="MQLFLAAGLLPASGAAAASSRPNILFLFADDQRADTIGAWGNPH
                     IRTPALDKLARDGCSFRRNYIFGGDRGAVCMPSRAMLMSGRSWFHVDTRKLSDAKLMP
                     ELLRENGYVTFGTGKWHNGEESWRRSFQQGQAVMFGGMSDHSRVPIRDLGPDGKLTAE
                     RTGEKFSSELFADSAIAFLRQHDGRRPFFVYVAFTAPHDPRMPPARLRDYYYRHQPPL
                     PANFLPQFPFDNGHLLKCRDENLAAWPRTEAAIRDQLAEYYGLITHMDGQIARILKAL
                     RQTRAARNTIIIYSADNGLALGSHGLLGKQNVFEHSMRVPLIFAGPGIPAGRSLAAFT
                     YLLDVFPTVCDLAGVPPPAGLEGESLRPLWEGRKTMLRDSVFLPYMQIQRAVRDERWK
                     LIAYPQIGHLQLFDLQMDPDERSNLIESPEYREHTRRLLERMRQWQAKTGDNLALPAT
                     NQPPVRIDLTGRARVPDQWQPEWIVKKYF"
     gene            complement(5375..5860)
                     /locus_tag="PKI07_05630"
     CDS             complement(5375..5860)
                     /locus_tag="PKI07_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50567.1"
                     /translation="MQNNFDFIGATDKPALVAFSTPEWLETTKTALAELGYKVHTAAT
                     HTDFLIRFSQVSYRVVILEELFAANDLSENLTLQALQNMPMSQRRHATIILLGSSFQT
                     FTPLQAFQQSVHAVINSSELFLLKQLIEKAVADNLFFLHSFREAQRQAYSGAAKNEVA
                     A"
     gene            complement(5870..7075)
                     /locus_tag="PKI07_05635"
     CDS             complement(5870..7075)
                     /locus_tag="PKI07_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOC50568.1"
                     /translation="MRRPELDYLLSTMLDSQPEVSDLLFTVDKPLQVESFGELKSVAC
                     DPAIDRLTPFQTEMIALNLMGDNQWHIKDLLQRGSCDTAYTLADKARFRISIFSQRGN
                     YSVVLRKLNTVIPTLEWLNFPDVIRQIPREKTGLVLVTGATGSGKSTTLAAILNEISH
                     TRPVHIITLEDPIEFVHPHALATFNQRELGSDFNTFSHGLRAALRQAPKVILVGEMRD
                     RETIKIALSAAETGHLVLSTLHTVDAGQTINRILGMFETDEQEQLRARLADTLRWVVS
                     QRLVPKVGGGRCALLEIMGSNLRTQETIRLGETEGKTFYEITEASQPFGWRTFDFSAL
                     EAYQQGKITEETALLYCTKRGPVTRGIDNIKKSRGESTHRVNSLRMRAVQDIAKPSRP
                     APPVMLKLK"
     gene            complement(7082..8176)
                     /locus_tag="PKI07_05640"
     CDS             complement(7082..8176)
                     /locus_tag="PKI07_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOC50569.1"
                     /translation="MAKIDAFFNLMFEQKASDLHLSSGNHPMLRVNGELHRVDHPPLE
                     NDELKKMLYEIAPEYKIKVFEEVGDVDFGYEIPHISRFRANFYNQKYGIGAAFRQIPT
                     KVLSFDDFEKFDAPLPSVLKKFCMLNRGLIVVTGPTGSGKSTTLAAMVDYANKNRRDH
                     IITVEDPIEFVHESKNCLVNHREVGVHTKSFAAALRGALREDPDIILVGELRDLETIE
                     LALVAASTGHLVFGTLHTPSAAKTVDRMIDVFPAEQQNKIRATLSEALKGVVAQNLFR
                     RVDKKGRIAALEILVFTTAIANLVREGKTHQIPGMIQVGKKIGNQPLDDAIMDHLRMK
                     RISAEEAYDKAFDKRKFRAFLPHPPEDDDL"
     gene            8300..9394
                     /locus_tag="PKI07_05645"
     CDS             8300..9394
                     /locus_tag="PKI07_05645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOC50570.1"
                     /translation="MELALYCPDCGYYDREKDSLGRRGDYYTSVSVGSLFGELLGWQF
                     AEWLEPGRAAGRKIRIVEAGAHDGKLAGDLLAWLRQYRPALFEPLQYWIVEPSRRRRD
                     WQRQNLAPFGDQVRWVAKLDELAPAASAAPRPQDSARGWEVIFSNELLDALPVHRLGW
                     DAQKRAWFEWGVALEAGRFVWTRMADKAPAPGAPTAEELLAFSWPGLPLDNDLRERLP
                     DGFSLEVCPDAAAWWRAAANALAGGKLVTIDYGLTAEELIRPERPAGTLRAYHRHQAS
                     HEVLAPPGEQDITAHVNFTAMQKAGESAGLQTEGLLTQAQFLTRIAAQTWAAENRFGA
                     WTAARIRQFHTLTHPEYLGRSFRVLVQSRG"
     gene            complement(9465..10214)
                     /locus_tag="PKI07_05650"
     CDS             complement(9465..10214)
                     /locus_tag="PKI07_05650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOC50571.1"
                     /translation="MHRFYMPPEQCRAPELRLTGPEAGHARRVLRVRRGERVVVLDGA
                     GGEFLCEVGDARRDEVRLAVVERRRHPEPASRITLLQAVPKGRIMEAIIQKATELGVA
                     RIVPLLTERVVTRLEAEEEATHKADKWQAAAREAIKQCGAPWLPQVEQPLTPWEFLAR
                     KESFELPLIASLAGGSRPAREYFNTFQATHHRRPQSLCVWIGPEGDFTAAETEAIQSG
                     GALPITLGPLVLRVETAAIYCLAILNYEMLA"
     gene            complement(10298..11422)
                     /gene="dnaJ"
                     /locus_tag="PKI07_05655"
     CDS             complement(10298..11422)
                     /gene="dnaJ"
                     /locus_tag="PKI07_05655"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOC50572.1"
                     /translation="MAKRDCYEVLGLERTTDAEEIKKAYRRLALKYHPDKNPGDKAAE
                     EKFKELGEAYEILSDPQKRALYDQHGHAAFDRRSGGFGGGGFHDPFEIFRDVFGGGGI
                     FEDLFGGGRLDPTRPQRGNDLRYDMEITFEEATRGCEKEITVTKPQVCDACRGTGAEP
                     GSRIRECPSCGGRGQVNISRGLFSIAQACSRCQGAGRVLDKPCRPCHGEGRRELPSKI
                     KLRVPAGVDTGARLRSVGNGEAGLRGGPPGDLYVVLHVQPHALFQREGDDLLCEVPVS
                     FVQAALGAEVEVPTLNGPSTIKIPAGVQPGTMFRLKGKGVKNVQGYGHGDLHVRIAVE
                     VPTRLSAAQRAKLQEFADLCSGKENPLSQGFLERARRMFQ"
     gene            complement(11426..12019)
                     /gene="grpE"
                     /locus_tag="PKI07_05660"
     CDS             complement(11426..12019)
                     /gene="grpE"
                     /locus_tag="PKI07_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOC50573.1"
                     /translation="MKRTKAHAETPPPADSSPSAADGAAAGEPLASAPASPLTAEQLA
                     ELQARAAKADEHWERLLRTAADFDNFKKRAAREKQEGIQFANEGLLNKLLPVLDSLDK
                     AVTAAQSATPEANEALHEGVNMISRQLKSVLAEAGLEEVDAMGQLFDPHLHEAISQLE
                     TPEVPPDHVAQQLRKGYRLRGRLLRPATVVVAKSPAA"
     gene            complement(12152..12856)
                     /locus_tag="PKI07_05665"
     CDS             complement(12152..12856)
                     /locus_tag="PKI07_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOC50574.1"
                     /translation="MKRRFAGLKWFPLPLAGCLLAGFLLVGCRAPLPNTLATHDSSRW
                     QKTIAAFQAMDATNRPPENCIVFVGSSSIRMWKTLAADFPGRPVVNRGFGGSQIADSV
                     NYAEELITSCRPRQVVIYAGANDISAGKSPELVLGDFVALVKKIRQGAPRARIAFISV
                     APNPARWAQVERVRQLNSLVRDYCARHRCAFIDVFPLMLGADGLPLPDIYVADRLHMN
                     PKGYAIWRQAVEPYLK"
     gene            13064..13810
                     /locus_tag="PKI07_05670"
     CDS             13064..13810
                     /locus_tag="PKI07_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOC50575.1"
                     /translation="MTTIIGSALVTLLLLAGRLPAQTPSTTALAAPLVPATRSTPTNW
                     LARHEGFVAQTQRGGIDLLFMGDSITDGWRKKGAAVWDHYYAPRRAANYGIGGDRTQH
                     VLWRLQHGELTGLKPKVTVLMIGTNNSKEDSPADIAAAIQLIVREIRARCPKTKVLLL
                     AVFPRNRPTDTPEQLATLRKINEIIARLDDGKKVRFLDINKVFLGPDGKVPADIMPDF
                     LHPNERGYQLWAEAMEPTLAKMLKTRPIRK"
     gene            14040..15011
                     /locus_tag="PKI07_05675"
     CDS             14040..15011
                     /locus_tag="PKI07_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50576.1"
                     /translation="MTPPISRRGFVKSSLIAAAGVPLALRGQTNPAPPTPSTPGAPAP
                     QPPLPTGRIGGQEFSRLMLGGNLIGGWAHSRDLAYVATLARRYNTDAKIRETFELAEA
                     HGITTINTWVMQENSHLFDHWKRGGKMKWIAQVRLDAGGGYSQITRAIDEGAAGVHIT
                     GDTAESLLAAGQFEKVGETLELIKAKKRLAGVAAHDLRVIIECEKAGLDVDFYQKTLH
                     SHDYYTAPRPGEKDALGANDNSWCSDPQAVIDFMATVKKPWIAFKILAAGAIHPRAAF
                     PYAFNHGADFILVGMFDWQIEENARLARRVIAAASGPASKRTRPWYG"
     gene            16135..17013
                     /locus_tag="PKI07_05680"
     CDS             16135..17013
                     /locus_tag="PKI07_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC50577.1"
                     /translation="MNTAPFRWCSRKAPQRARGFTLIELLVVIAIIAILAALLLPALT
                     RAKLKAQGVQCMNNHKQLALAWRMYVEDSSDRLPYASSWPVGTWMAETWCNGRMDFAA
                     GNRSNWDPTVDIHRSPLWPYCGKNLGIFHCPADRAYVVVGAERKTRLRSMAMNAYVGG
                     FAGKPIATGNMAAYTVYTKYSAMDRPGPDRIFVFIDEREDAINWGNYLQDMTGYSPPN
                     PRMYRWLDLPAAYHGNAGGLSFADGHSEIKKWRDGRTSRPIKEQGAIFDGSTPIPQDG
                     NMDIGWMQDRATRPKL"
     gene            17029..19377
                     /locus_tag="PKI07_05685"
     CDS             17029..19377
                     /locus_tag="PKI07_05685"
                     /inference="COORDINATES: protein motif:HMM:NF022752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOC50578.1"
                     /translation="MGPKRSFAAAALLLSAGLLAARGAFAGGLICLQSNSCQARVDPS
                     RLEVALRNGSQPEMIVSAAQTNLGRVADLRFDRELNPGTASWTLPERKVTLTFTVTEN
                     RLAVHVRATEPGEFTFPVIPAGGPAKAWILPLFEGVYAPCGDTNWETFLTTHGALNTT
                     ADLVLPFVGLECRAAAPARAWTLTCILTNPFNNQLQFVRAPDSTLAARLTHQFTRNHP
                     VKEYAVVFELGPPSPIEPARRYRQWLIERGEFVRFSDKIRKTPEAAKLPGAAHVYLWG
                     NDLLNQADVTDWKGFARALQAGAAAGASPPVRWIWDQLKPDTRTVAAQILAAAWPDRY
                     QKSQIIEDLNRLLEQPEFAGAAAGLEAALEPEIAALLKSDRRSLSRADRSRLNTHLLA
                     AAFPGLLAQPDAWGNGTSPQMIQQLAAAGLDRLWLGSDGWRGFQERPQTVAAAKKAGY
                     LIGPYDSYHSIHRPGEPDTWETAQFGAALYETGAIVNADGTRRRGFKQKGFLLSPAAA
                     RPYVEKRVAALMPSFHANSWFIDCDGFGDYFDDYAESHPATQQSDLQARVSRLAWIRD
                     TFGVVIGTEGCSAGVAATVHFAHGVLTPVIGWGDPDLTDKQSPYYLGSYYPPDEPRVF
                     FQPVPMKAKYRYLYYNPGFRLPLFETVFHDSVIATHHWMFASLKAREDAPTVELLELL
                     YQVPPLYHLNRAEFQKRRAQIKRHFEFFSPLHRETALLPLADFQWLTPDRRVQRTVFG
                     DRFEVIANFGPAEFRRDDARLGPRSLQVRHLLTGRTQTYTAQ"
     gene            complement(19405..19791)
                     /locus_tag="PKI07_05690"
     CDS             complement(19405..19791)
                     /locus_tag="PKI07_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50579.1"
                     /translation="MLVFLAVLTSACSTPRGAHTTRFKDQEHASLIVRYYTDDTNYVL
                     KPEAKEGPFLSILDQNGVLAVARQQTGRDLAVVVLIHYAGENQANFVKSKWRTLLTEA
                     GYRRVVFLRGRTGMRVNGLPVLSSPS"
     gene            20155..21030
                     /locus_tag="PKI07_05695"
     CDS             20155..21030
                     /locus_tag="PKI07_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOC50580.1"
                     /translation="MRLCLLLMTMICLSGCQSASVRTDAVCQSYTLDYDTPTDPALQA
                     ELEALDARLRERCGMTAEPTAVGVLDLKRLRLALINPDRIDYAASVAKVGILLAYFQL
                     HPDAATHLDPVVRHELGLMAKASSNEMAAKYSRQMGLRQIQEVLNSYHFYDARRGGGL
                     WVGRHYGVDGERIGDPLADHSHAMTVRQVLRFYLLLEQGKLVSPQASQTMREIFAAPD
                     IPHDDIKFVKGLAGRDVRILRKWGSWKDWLHDSAVITGPGRHYILVAMTHHPNGDQYL
                     ADLAGAVDDLLARRP"
     gene            21196..22491
                     /locus_tag="PKI07_05700"
     CDS             21196..22491
                     /locus_tag="PKI07_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC50581.1"
                     /translation="MWAAACGGGQHAALGAPKVKPPKGFTALFNGQDLTGWRGGETFD
                     PRKLQALPPEQRAAQIAKWTESMKTHWHAEKGELVNDGQGAYATTEKDYGDFELLVEY
                     KTVPRADSGIYLRGCPQVQIWDYTDAEKFPLGANRGSGGLWNNSPGAPGKDPLVLADK
                     RFGKWNACRIVMVGARVSVWLNGKCVVDHALMENYYDRQTPVPPTGPIALQTHGGEIR
                     WRNIFLREIGAAEANQILARHGNAGFKSIFNGRDFRGWAGPTNNYQVVDGTIQCLPKK
                     GGTIYTTEEYRDFAARLEFKLPPGGNNGLAIRYPGEGDTAYVGMCELQVLDDHYEQVT
                     GHALDPRQAHGSAYGMVAAARGYQRPIGEWNFQEITVQGSKLKVELNGTLILNCDLAG
                     VKEFMGDRPHPGKDRTSGYFGFAGHNDPVAFRHIAIKPL"
     gene            22568..23644
                     /locus_tag="PKI07_05705"
     CDS             22568..23644
                     /locus_tag="PKI07_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50582.1"
                     /translation="MVAILASSRISRRLPACLGAIALALLGLGLTGCRHVEPDSCAPP
                     ATNAPPVVAPQPPAATNATASKPAETPKRYSYTPRVYPVDERGFSPVDKALAKAYARE
                     DMVADDAEGSLNPYVMKVISAYPLDGSYPYHCGWTPREYDLYNGVTQDLWYKGMVVAK
                     AYPDGSRCSYCCGLTFEIFVRAMKLRNVQKGLDPDDFNGMTFSDLFNLLQLWYIEGPG
                     DCEQRGITSYGLGRAITNFEEVRPGDFLSYSTTPAGGHSVVFIDWIRDEQNKIVGLKY
                     FSSNLSGTRGVGYGSGRFSDSTSTGRGLLRKSLRIARVGAIKDYKPFDRAAIPLRNAY
                     LPTQPTRIIYLPAPAAAPAPDAAR"
     gene            complement(23706..25148)
                     /locus_tag="PKI07_05710"
     CDS             complement(23706..25148)
                     /locus_tag="PKI07_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC50583.1"
                     /translation="MNTEQLYQQRLHRYITALRNEKPDQVPLRPFVAEFTAQYAGYTC
                     QEVAHDYNKAFEAALVTARDFGWEAVVPNMVYVWTGLAQAVGLRYYGIPGIGIPHTTG
                     FNYIEPPEEQAWMRADEYDALIADPTAFLYNVWLPRVSTEVSPPGAPATYRHNLSFVK
                     GAMAMLSYFYAFGPAIARLRTESGTVSAIAGIFKAPFDILADKLRGYVGLTMDMHTQP
                     QKVLQACEALMPHLCHVGLTTADPARQVPIGFWMHRGCVPFINPRTFASHNWPTLRPI
                     IEEFWRQGHQTLFYAEGKWHHHFDAFRELPDRSIVFHCDQDDVFLAHRKLHDKFALSG
                     GIPNALLSYGQPEEVRAFCRRVISEVARDGGYIMDAGAIMQNDTRVENIRVMTDTARQ
                     YGVYSAGSYSPPTATPPCDLPSSVADRQQLTGLQGRPAPHVRPGVCFPWEERLKDLPE
                     ITGSPELIRRVWEDIDAFGNMYIWQLLLSF"
     gene            25275..26057
                     /locus_tag="PKI07_05715"
     CDS             25275..26057
                     /locus_tag="PKI07_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC50584.1"
                     /translation="MKIWDAHCHLSGVPGQTPAERMAQLLSFADRLGIERLCVCMGMR
                     WSYDPTPADMRQQNDEVLAALQHWPHRALGYVYLTPNHVAASLAELDRCVRDGPMVGV
                     KLWVARRAHAPGLDPIVERATELQAPILQHTYLKATGNLPGESTPMDLAELARRHPAA
                     TFIGAHAGGDFELGLRALRACENVSVDLCGTDPAAGMTEMAVRELGAHRVLYGSDAGG
                     RSFASQLGKVLGADIPAAAKKLILGENLRRLLRPILQRKKSL"
     gene            26054..27001
                     /locus_tag="PKI07_05720"
     CDS             26054..27001
                     /locus_tag="PKI07_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC50585.1"
                     /translation="MTSMRQKDGGADPGRLSRRSFLCTTTVAAAAAAAAGWRAETADA
                     AAPDPLIGATGPRGLVDTNVWLGQWPGRRLPLEETPALVARLRRQGVRQAWAGSFDGL
                     WHKDLGAVNARLAAECRRQGRGLLTPFGTINPADPAWEEDLRRCAEEHQMPGIRLHPN
                     YHGYQLDDPALARLLDRAGQRGLIVQLVAAMEDERTQHPRMRVPQVDVAPLAALLASR
                     PELRLVLLNWGRGANSVQPPRLAEAGQICFDIATLEGVGGVASLLRQVSAGRVVFGSC
                     APLFYLESALLKLQESPLTEAQLAAVRADNALRLRRRVS"
     gene            complement(27024..27623)
                     /locus_tag="PKI07_05725"
     CDS             complement(27024..27623)
                     /locus_tag="PKI07_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOC50586.1"
                     /translation="MKRILALWAGLGLLSLSLAAAEVGQPAPDFSGTDIHGRTHKLGD
                     YKGKIVVLESYNLDCPYVRHQYRSGAIQELQRDLGAQGVVWLLVNSVSPKHPGYRRPE
                     AAQKEWTAQKIAAAAWLDDGSGRIGKAYGMKTTPHLFVIDQAGVLAYQGALDDRAATE
                     GDPRTARNYVREAVQKLKAGERPVVTQTKPYGCGVKYGN"
     gene            complement(27751..29892)
                     /locus_tag="PKI07_05730"
     CDS             complement(27751..29892)
                     /locus_tag="PKI07_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOC50587.1"
                     /translation="MYSMFGRRFLSLFGVFWLGLGGLSARAAHTQAQLLLDAEVARPG
                     DTVLAGIHLRMDPHWHTYWQNPGESGMATAVEWQLPAGVAAGDIQWPVPEKYLESDLV
                     TYVYTHEVVLLVPLRLAADLRTGPLELSAEVSWLECEVACVPGKARVRATLIVGAETK
                     ASKESARLKAWQGKLPQRGGGLSVRAWWDSVPVGNSRTLMLEWKPAAGVTEADFFPDA
                     SEEFEVQPATEKAESDPGILRLRKKVTKLRGDWPRHVSGLLVQEAGAARRADEVRLPI
                     RDSESDARDGRAPSAGAGLGTPALWKMLLYAFLGGLILNVMPCVLPVIALKILGFVGQ
                     ARDEPRRVRQLGLIYALGVLVSFLVLAGLVIGVKAAGHKAGWGMQFGNPQFLVLLTML
                     VTLVALNLFGLFEINPGGRVLGAAGALAAKGGPAGAFFNGVLATILATPCTAPFLGAA
                     LGFAFSQPAGLIIVMFLVVGLGLAAPYVVLSWHPAWLKFLPKPGAWMERFKVAMGFPM
                     LATAVWLFSLIPLHYGRRALWLGLFLVVLALAAWVYGEFFQRGRTRRGLGLAVALVLL
                     AGGFLYAVEGQLRWRAPVADASAENPGLPGGIEWQRWSPAALAAARAQGRPVLVDFTA
                     DWCLTCQANLRVAIDIPSVRARLKQINAVALLGDYTRLPDDITAELNRYGRAGVPLVL
                     VYPRNPAEPPQVLPEALTPGIVLDALARAAL"
BASE COUNT         5544 a         9977 c         9691 g         5388 t
ORIGIN      
        1 ccccagtggc ttaccatgac ccgctgcttt ttgctgaact tttaggacac taccctgaaa
       61 tgcgcgctat cctggccctg ttttagccga aacgattccg ttaagccgtc ccaacgaacc
      121 cttcggttgg cgaattcatt ggcataacca ttgagtttcc aatccctttc gccggtaaaa
      181 tcaataggtt aggcatagaa aggggtaaaa tagaggcaaa atccgaatca tgccgacttc
      241 ggcttacctt ggaagctccc cagccacccc ctacttccaa gccgtgtaca aggggtgtac
      301 aacggatgct catcgggtgt ccataggatt ttagctggta catccgatgg gcatcccttg
      361 tacacccctt gtacacggcg caggggcagg gtatatccaa gatgtccgtt gggtaatggc
      421 tacggatgcc tgaaaaggct gaaacgagga gaaaggacgg ggggataatt gttaagctct
      481 gtaaaaacag ttggttagga atctggggct ggcaaaaagc ggtttttcag agggggacgg
      541 gcttccggac gccttttggc ttcgcgaatg gcttatctga aacgttgccg tcggggattc
      601 cgataaaccg agatacgccc ggcagccacc ccctgcccgg gcgggcctcg cccactggga
      661 agggggcaaa actcatatcc aagtaatgac ttgagtcttg gccggcttat ctcgtctcgc
      721 ggcgggccag cacggcggcg cctgtgatgc cggcggcatc gcccagtttg gaggcgatga
      781 tctcgacgcc tttcatggtt ccgggcattg cgtagtcccg cgcggttttt acgatgatgc
      841 tcatcatctc gtcggccagg gcttccatga cgccgccgcc cagcacgacc acttccgggc
      901 tgaagaggtt gacgaggttg gccacgccga tgccgatata ttcggcggcg tcttcgacga
      961 tatgggctac aaacttatcg ccgcggcgga tggctttgcg gaggtcgccg ctgcgcaggt
     1021 cgtccaggtg gtcgcccagc atatccgtga ggagggtctt tgcgccgtct ttgacgcccg
     1081 ctttgatttg ctggaagatg gcggtacggc tggcgagcgc ctcgaagcag cctttgttgc
     1141 cgcagccgca tttggggccg ttgacgtcca ggaccatgtg cccgatttcg cccgccgtat
     1201 ggttgaagcc ggcgtagagg ttgccgttga tgataaggcc gccgccgatg ccggtgccca
     1261 caaagatgcc gatgaggctc cggggtttgg ccttgagttc ggccacgtgc acgcccaggg
     1321 cggcgatgtt gcaatcgttc tcgatgaaca ccggcaggcc gagctgcttt tcgaggcgct
     1381 tcttgagcgg cacatctttc cagccctcca tgttgggggc gaagatgacc cggccttcgg
     1441 cgaagttcac cgcgcccggg gcgccgacgc cgacccccgc cacctgtttg aggggcaggt
     1501 ccgcttcgtc cagcgcctcg cggacacagc gggcgacgcg ctcgatgacg gcggggacgc
     1561 cgcgttggga tttggtgctg agcttggcgg tgccgacgca ttccagcgag ttccggaaca
     1621 cgcccgaaag aatcttggtt ccgcccaggt ccacgcccac atagtatttg gtcttgcttg
     1681 aggtttccgc cataacccgc tcctatttgc ccgccagcac ggtgccgatc ttcttgcgca
     1741 gttcggcgtt gatgacttcc gccggacgca gcccgtccac gatggtaaag ccgtaggagg
     1801 cctgcaggct gcggaaggtt ttttgcattg cggtctggta tttcataaaa ctgtcaaaca
     1861 tgtcccgcga caaccccaga tccatgccgc tttcccaata atcgagcgtc gcgttcttgg
     1921 caaaggtgcg ctgcaccagc tcggccggcg ggacatggag atagaacacc acgtccggct
     1981 ccagcgccag gccgtagagg ttgcgcagcc aggcttcgtc catgccccgg accatatcgc
     2041 gcgccatgag ggtgtagatg tagcgatcgg ccagcacgac aaagccggcc ttgagggcgg
     2101 gcaggatgct gttttcgagc tggtcggcga agtccgtcgc atagaacagg ctcaaggtgg
     2161 tgcggctcag gatgttgccc tgctgcgccc gctgcaattc ctcgctcacg agcgtggatc
     2221 gcttgagccc cacctgcgcg gtggcgtggc cgcggttctc cagccattcg accagcaaat
     2281 caagctgggt cgagcgcccc gagccgtccg cgccctcgat gacaatgagc gttccggcca
     2341 gccgctccgg gtccacgccg ggcagcccgt gcccgtaaaa gcgccgggcg ccgcggcggc
     2401 gggccatctt ttgattttga ccggctgctt tcatgattcc gtttcctccg gttcgacggc
     2461 cttctcgggc ggcggcgggg ttttgcgggc gggccgttcc gcgcagcgaa acctggccag
     2521 gttcacccgc tgcgcgacca gcttgcgcac cacggcctgc tgattctcca ccggctcgtt
     2581 cgcattcacc accaggaagt tgaactcggt gctcagcgcc aggtactgct ccaggatgcg
     2641 cccctggaaa atgcgaaagc tctcatgcgg atcgggcgac aggcgcaggt ccatgcccgc
     2701 ttcgaagtac ttcaactgcg cccggccatc cagaatgcgc ttgagcgaga cctccaggtc
     2761 cgccttgaag aaaaaggtca ggtccggcag ggccgcgaag ctgtagatgc cccgcaccca
     2821 ctccggcggg cagccgcgca ccacgtcgcg cccgaaggcg gtgaagatgt agcggtcgca
     2881 caacaccagg tagcccgccc gcagcagggg caccaggtgc cgctcgtagc ggtcggcaaa
     2941 gtccgtcgcg tggatcaggc tgaacgtcgt gggggtcagc aactcgcgtt tcttgccttt
     3001 gctcgtggcg gatttcacca gttccgagga gttccactcg ctgaagtaca ctttcaaccc
     3061 ctgcgactcc agccagcgct tcagcaggta aatctgcgtg gatttccccg agccatccaa
     3121 gccctcgacc gcaatcagcc ggccggggaa atcgagttcg gcaaaggtct tcatgtcact
     3181 cgtcgcaggc taagccgcgt tttccggaat acaactgttt tttgcccgcg ggttttcggg
     3241 gccgcccctc cggcccgcgg ggcgcccggc ggggcgaaag gcccgcgcga cttcccggag
     3301 cactccggat ccgtgcgcag aatcggcttg cgcccgcggc ccgatggggg gatactgcct
     3361 gctgatgtcc gccgccacgc gcttggcggg caccgcgatg gaatgcgcgc ttagctcagt
     3421 ggaagagcac ttccttcaca cggaaggggc cgcaggttca agtcctgcag cgcgcaccat
     3481 tcggccggaa cgccgtttcg ggacgattcc cccggcctgt cagcttgtgg cgaacctggc
     3541 ggaagccggg gatggggcgc gatgttaagg ggcgggctgg cgaggctcag aagtatttct
     3601 tcacgatcca ctccggctgc cattggtccg gcacgcgcgc tctgccggtc aggtctattc
     3661 ggaccggggg ctggttggtc gcggggaggg cgaggttgtc gccagtctta gcctgccatt
     3721 gccgcatccg ttcgagcagc cgccgggtgt gctcccgata ttccggggat tcgatcaggt
     3781 tggagcgctc atccgggtcc atttgcaggt cgaacaactg gagatggcca atctgcggat
     3841 aggcgatgag tttccatcgc tcatcccgga cggcgcgctg gatttgcata tacggcagga
     3901 acaccgagtc gcggagcatg gtttttcttc cctcccacag cgggcggagg ctttccccct
     3961 cgagcccggc gggcggaggc acgcccgcga gatcgcagac ggtgggaaaa acatccagca
     4021 gataggtgaa cgccgccagg gatcggccgg ccgggatgcc cgggccggcg aaaatcagcg
     4081 gcacgcgcat gctgtgctcg aagacgtttt gcttgcccag caacccgtgg ctgcccagag
     4141 ccaggccgtt gtcggcggag taaatgatga tcgtgttgcg cgccgcgcgc gtttggcgga
     4201 gcgccttgag gatgcgcgcg atctggccgt ccatgtgggt gatcaggcca tagtattcgg
     4261 ccaactgatc gcggattgcc gcttccgtgc gcggccaggc ggcgagattc tcatcccggc
     4321 acttgagcag gtggccgttg tcgaagggga actgcggcag gaaattcgcg ggcaggggcg
     4381 gctgatggcg gtagtaatag tcacgcagcc gcgccggcgg catccgggga tcgtgcgggg
     4441 cggtaaaggc cacatacacg aaaaacggcc tgcggccgtc gtgctgccgg aggaacgcaa
     4501 tcgccgaatc ggcaaatagc tcgctggaga acttctctcc ggtccgctcg gccgtcagct
     4561 tgccatccgg gccaaggtcc cgaatcggaa cgcgcgagtg atcggacatg cctccgaaca
     4621 tcaccgcctg gccctgctga aacgaccgcc gccaggattc ctcgccgttg tgccacttgc
     4681 ccgtcccgaa ggtgacgtag ccgttttccc ggagcaactc gggcatcagc ttggcgtcgc
     4741 tgagcttccg ggtgtccaca tggaaccagg accgtccgct catgagcatc gcgcggctgg
     4801 gcatgcaaac ggcgccccga tcgcccccaa aaatgtaatt gcgccggaag ctgcagccgt
     4861 cccgcgccag cttgtccaat gccggggttc ggatatgcgg gttgccccag gcgccaatgg
     4921 tgtccgcccg ctggtcgtca gcaaacagga aaaggatatt cgggcggctg ctggcggcgg
     4981 ccgcgcctga agcgggcagc aaacccgccg ctaaaaacag ttgcacgccc caaaccagca
     5041 accgggggca actcttccga ttttccgccc ttgagacacc gcgaaacata aagcgttgtc
     5101 gttgtgattg tgattgtccg cctcacgctg ccttcgttca ccggatgggt caagggaatt
     5161 gcgagccact ccggcaggtc cgggacgtca gccaatcgct ccggttactg ccggcccgat
     5221 cgcgcagtcg ttgcgccacc gcagcccggg cggcttgcct ccgggaaaaa ctgttcagcg
     5281 atccgggttg tcttaccgcg ctctcgcaga caataccgcc aatccttcgg cctccggacg
     5341 tctgtccagc ccgtgcgttg ccccggggcg gcgtctaggc cgcgacctcg tttttggcgg
     5401 ccccggaata ggcctgcctt tgcgcctcgc gaaagctatg caggaagaaa aggttgtccg
     5461 caacggcctt ctcgatgagc tgtttgagga gaaataattc cgagctgttg ataaccgcgt
     5521 ggacgctctg ctggaacgcc tgcagcgggg tgaaggtctg aaaggaactg cccaggagaa
     5581 taatggtggc gtggcggcgc tggctcatcg gcatgttttg cagcgcctgc agggtcaggt
     5641 tctcgctcag gtcattggcc gcgaagagtt cctccaggat gaccacccgg tagctgacct
     5701 gggaaaaccg gatcaggaag tcggtgtgcg tggcggcggt gtggactttg tagcccagtt
     5761 cggccagggc cgtctttgtg gtttccagcc attcgggggt ggagaaagcc accagggcgg
     5821 gcttgtcggt cgcgccgatg aaatcgaagt tgttttgcat gaggcctggc tacttgagtt
     5881 tgagcattac cggcggcgcc gggcggctcg gtttggcgat gtcctggacc gcccgcatgc
     5941 gcagcgaatt gacgcgatgg gtggactcgc cccggctttt cttgatgttg tcaatgcccc
     6001 gggtgaccgg gccgcgcttg gtgcaataaa gcagggcggt ttcctccgtg attttgccct
     6061 gctggtaggc ttcgagcgcg gaaaagtcaa aggtgcgcca gccgaacggc tggctggcct
     6121 cggtgatttc gtagaaggtc ttcccttcgg tttcacccaa tcggattgtt tcctgggtgc
     6181 gcagattgga ccccatgatt tcgagcagcg cgcaccggcc gccgccgacc ttgggcacca
     6241 atcgctggct gacgacccag cgcagcgtgt cggccaggcg ggcgcgaagc tgctcctgct
     6301 catccgtctc gaacatgccc agaatacggt taatcgtttg cccggcgtca acggtatgca
     6361 gcgtgctgag caccaggtgc ccggtttcgg cggcgctgag cgcgattttg attgtctcgc
     6421 ggtcccgcat ttccccgacg aggatcacct tgggcgcctg ccgcagggcc gcgcgcaggc
     6481 cgtgcgagaa ggtgttgaag tcgctgccca gctcgcgctg attgaacgtg gccagggcat
     6541 ggggatgcac gaactcgatc gggtcttcga gcgtgatgat atgaacgggc cgggtgtggc
     6601 tgatctcgtt gaggatggcc gccagggtgg tggatttgcc cgagcccgtg gcgccggtga
     6661 cgagcaccag gccggtcttt tcccgtggga tttgccgaat gacgtccggg aagttgagcc
     6721 actcgagggt ggggatgacg gtgttcagct tgcgcagcac cacggaatag ttgccgcgct
     6781 gcgagaaaat gctgatgcgg aagcgcgctt tgtcggccag ggtgtaggcg gtgtcgcagg
     6841 agccgcgctg gagtaaatcc ttaatatgcc actggttgtc gcccattaag ttgagcgcga
     6901 tcatttccgt ctggaacggc gtgagccggt caatggccgg gtcgcaggcc acggatttca
     6961 attcaccaaa cgattcgact tgcagcggct tgtcaacggt gaagagcaaa tcggacactt
     7021 ccggctgcga gtccagcatc gtggacagga ggtagtctag ctcggggcgg cgcatataag
     7081 atcagaggtc gtcgtcttcc ggagggtgcg gcagaaacgc gcggaacttt cgtttgtcga
     7141 aggctttgtc gtaggcttct tcggcggaga tgcgcttcat gcggaggtgg tccataatcg
     7201 catcgtccag cggctggttc ccgattttct tgccgacttg aatcatgccc ggtatctggt
     7261 gggtcttgcc ttcgcgcacc aggttggcga tggccgtggt aaagaccagg atttccagcg
     7321 ccgcgatgcg ccccttcttg tcgacccgcc tgaagaggtt ctgcgccacg actcctttga
     7381 gcgcctcgga aagggtcgcg cgaatcttgt tctgctgttc cgccgggaaa acgtctatca
     7441 tgcggtccac cgtcttggcc gcgctgggcg tatggagggt gccgaagacc aaatggccgg
     7501 tgctggctgc caccagggcc agctcgatgg tctccaaatc acgcaattcg ccgaccagga
     7561 tgatgtccgg gtcctcgcgc aaagcgccgc ggagcgcggc cgcaaaggac ttggtgtgaa
     7621 cgcccacctc gcgatgatta accaggcagt ttttgctctc gtggacgaat tcgatcgggt
     7681 cttcgaccgt gatgatgtga tcgcgccggt tcttgttggc atagtccacc atcgccgcca
     7741 gcgtggtgga tttgccggag ccggtcgggc cggtcaccac gatcaggcca cggttgagca
     7801 tgcagaactt cttcagcaca ctcggcaggg gcgcatcgaa cttctcaaag tcgtcaaagg
     7861 agagcacctt ggtcggaatt tggcgaaatg ccgcgccaat gccgtatttc tggttataga
     7921 aattggcccg gaaccgggag atgtgcggga tctcataccc gaaatcaacg tcgccgacct
     7981 cctcaaagac cttgattttg tattccggcg caatctcgta cagcatcttt ttaagctcgt
     8041 cgttctcaag gggcggatgg tccacccgat gcagttcgcc attgacccgc agcatggggt
     8101 gattccccga ggaaaggtgc aggtccgacg ccttttgctc gaacatcaga ttgaagaatg
     8161 catcgatttt cgccataaac gtccgtatag ttgcactcct gatagctaaa tccgcaccaa
     8221 gtccaccgaa ttgagcaaag cggccaaaag aatcgcagaa gaaataaacg cgcggggcgc
     8281 catcccgttc cggcgtttca tggaattggc cctgtattgt cctgattgtg gatactacga
     8341 ccgggaaaag gacagcctcg ggcggcgtgg ggattactat accagcgtaa gcgtggggag
     8401 cctgttcggc gagttgctcg ggtggcaatt cgctgagtgg ctcgaaccgg gccgggcagc
     8461 cggacggaaa atcaggattg tcgaggcggg cgcccatgac gggaagcttg ctggcgacct
     8521 gctagcctgg ctgcgccagt accgcccggc gctgttcgag ccgttgcaat actggattgt
     8581 ggagccctcg aggcggcggc gtgactggca acggcagaac ctggctccgt tcggggacca
     8641 ggtgcgttgg gtggccaagc tggacgagtt agctcccgcg gcgagtgccg ccccacgccc
     8701 gcaggattcc gcaagaggat gggaagtcat cttctccaat gagttgctcg acgccctgcc
     8761 ggtgcatcgg ttggggtggg atgcgcagaa acgcgcctgg ttcgaatggg gagtggccct
     8821 cgaagcgggg cgcttcgtct ggacgcgcat ggcggacaag gccccggccc cgggagcccc
     8881 gacggcggag gaactgctgg ccttttcctg gcccgggctg ccgctcgaca acgatttgcg
     8941 ggaaaggctg ccggacggat tcagcctcga agtatgcccg gacgccgcgg cttggtggcg
     9001 cgcggcggca aatgccctgg cgggaggcaa gctggtgacg attgactacg gcctgaccgc
     9061 cgaggaattg atccggccgg agcgcccggc gggcacgctg cgggcttacc accgccacca
     9121 ggcgagccac gaggtgctgg cgccgcccgg cgaacaggat attacggcgc atgtgaactt
     9181 cacagccatg caaaaggcgg gcgaatccgc cggcctgcaa accgagggcc tgctgaccca
     9241 ggcgcaattc ctgacccgca ttgccgccca gacctgggca gcggagaatc gcttcggcgc
     9301 atggacggcc gcgcgcatac gccagtttca caccctgacg catccagagt atttggggcg
     9361 ctcatttcgc gtgctggtgc aatcgcgggg ttaaccgctc cgggacgccg cccggctttc
     9421 tcccatccca ccccggcttg tccgggcgcg ggccgagccg gtgactacgc cagcatttcg
     9481 tagttcagaa tcgcgaggca ataaatcgcg gcggtttcca cccgcaagac caggggccca
     9541 agcgtaattg gcaacgcgcc tccggactgg atggcctcgg tctcagcagc ggtgaaatct
     9601 ccctccggcc cgatccagac acacagggac tgcggccggc ggtggtgggt cgcctggaag
     9661 gtgttgaaat attcccgcgc cgggcggctg ccgccggcca gcgacgcaat caacggcagt
     9721 tcaaaggatt ccttccgcgc caaaaactcc cagggagtca gcggctgctc aacttgcggc
     9781 agccagggag cgccgcattg tttaatggcc tcccgcgccg ccgcttgcca cttgtccgcc
     9841 ttatgcgtgg cttcctcctc cgcttcgagc cgggtcacga cccgctccgt gagcagcggt
     9901 acaatccgcg ccacccccag ctccgtcgcc ttttgaataa tggcttccat gatccgccct
     9961 ttcggcacgg cctgcagcag ggtgatgcgg ctggccggct cgggatgccg ccgccgttcc
    10021 accaccgcca aacgaacctc gtcgcgccgg gcgtccccca cttcgcagag aaactcgccc
    10081 ccggctccgt ccagcacaac gactcgctcc ccgcggcgga cccgcaaaac ccgccgcgca
    10141 tggccagcct ccggcccggt caggcgcagt tcgggagcgc ggcactgttc cggcggcata
    10201 tagaagcgat gcatgggagg acaacaacgt tgccgtgaaa agcgcgcgcc gcaaccgcca
    10261 gcgggtcacg cgtgatccgg cggccccggc tttcgtttca ttggaacatg cgccgggccc
    10321 gttcgaggaa cccctggctg agcgggtttt ccttgccgct gcagaggtcg gcgaattcct
    10381 gcagcttcgc ccgctgggcg gccgacaacc gcgtgggcac ctcgaccgcg atgcgcacgt
    10441 gcaaatcccc atgcccatag ccttgcacat tcttgacgcc cttgcccttg aggcgaaaca
    10501 tcgtgccggg ctgaaccccg gcggggatct tgatcgtgga cggcccgttc aaagtgggaa
    10561 cctccacctc cgcccccagc gccgcctgaa caaaactgac cggcacctca cacagcaggt
    10621 catctccctc ccgctgaaag agggcatggg gctggacatg gagcacgacg tataagtcac
    10681 ccggcggccc gccgcggagg ccggcttccc cgttgcccac cgagcgcagg cgcgcgccgg
    10741 tatccacgcc ggcgggaacg cgcagcttga ttttggacgg caactcgcgc cgcccctcgc
    10801 cgtggcaggg ccggcagggt ttatcgagca cccgccccgc cccctggcag cgcgagcagg
    10861 cctgggcgat gctgaacagg ccgcgcgaaa tattgacctg gccgcgcccc ccgcaactcg
    10921 ggcactcccg aatgcgggat cccggctccg cgcccgtgcc gcggcaggcg tcgcacacct
    10981 ggggtttggt gaccgtgatt tccttttcgc acccgcgcgt ggcttcctcg aaggtaatct
    11041 ccatgtcgta gcgcaaatcg ttgccccgct gcggccgcgt cggatccagc cggcccccgc
    11101 caaagagatc ctcgaagatt cccccgccgc caaacacgtc gcggaagatt tcaaaggggt
    11161 catgaaaacc gcccccgcca aagccgcccg accggcggtc aaacgccgcg tggccatgct
    11221 ggtcgtacag ggcgcgcttc tgcgggtcgc tcaggatttc gtaagcctcg cccagttcct
    11281 tgaacttctc ctccgccgcc ttgtcccccg ggttcttgtc cgggtggtat ttcaacgcca
    11341 gccgccgata cgccttcttg atttcctcgg cgtccgtggt gcgttcaagc ccgagaactt
    11401 cgtagcaatc tcgcttggcc atgactcagg cggcggggga cttggcaacg accaccgtgg
    11461 cggggcgcaa aagccggccg cggagccgat agccctttcg gagttgctgc gccacgtggt
    11521 ccgggggcac ctccggcgtc tcaagctgtg aaatcgcctc atgcagatgc gggtcaaaca
    11581 actgccccat cgcgtccacc tcttccaacc cggcctccgc cagcacgctc ttaagctgcc
    11641 ggctgatcat gttgacgccc tcgtgcagcg cctcattcgc ctcgggcgtg gcgctttgcg
    11701 cggccgtcac cgccttgtcc aggctgtcca gcaccggcaa aagcttgttg agcagcccct
    11761 cattggcaaa ctgaatcccc tcctgcttct cgcgggcggc gcgcttcttg aaattgtcaa
    11821 aatccgccgc cgtgcgcagc aggcgctccc aatgctcatc cgccttggcg gcgcgcgcct
    11881 gcaattccgc cagttgctcc gccgtcaggg gagacgccgg ggccgacgcc aaaggctccc
    11941 ccgccgccgc gccgtccgcc gcggagggcg aactgtcggc gggtggcggt gtctcggcat
    12001 gcgctttggt tcgtttcata aagctcggct tacctatcca cgatttcaat ggggggagaa
    12061 taaacgattg cccccggaac ggcaacccgg attgctcgcg gcgcccggcc ggaaccgcgg
    12121 gcgcaccctc cttcgcgggc cgcccccgcg cctatttcag atacggctcg accgcctggc
    12181 gccagatcgc atagcccttc ggattcatgt gcagccggtc ggccacatag atgtccggca
    12241 ggggcaaacc atccgcgccc agcatcagcg gaaacacatc aataaacgcg cagcgatgcc
    12301 gcgcgcaata gtcgcggacc agcgagttaa gctgccgcac ccgctcgacc tgggcccagc
    12361 gcgcgggatt gggcgcgacc gagataaagg cgatccgggc ccgcggcgcc ccctgccgga
    12421 tcttcttcac cagcgccacg aaatccccca aaaccagttc cggcgatttg cccgcgctaa
    12481 tatcattcgc gccggcataa atcacgacct gccgcggccg gcaggacgtg atgagttcct
    12541 ccgcgtaatt gaccgagtcc gcaatttgcg agcccccgaa gccccggttg accaccggcc
    12601 gcccggggaa atcagcggcc agcgtcttcc acatgcgaat gctggagctg cccacaaaga
    12661 caatgcagtt ctcaggcggg cgattggtcg cgtccatcgc ctgaaacgcc gcaatggtct
    12721 tctgccagcg gctggaatca tgggtcgcca gggtgttggg cagcggcgcc cggcagccaa
    12781 ccagcagaaa acccgcgagg agacaacccg ccagcgggag cgggaaccac ttcaagcctg
    12841 caaatctacg cttcatatca aggcgacagc ttaaacggct ctccgggcca ggcaagccgc
    12901 aatcgccgga cggcgcatga aatacccggg cggacccgcg gagccgcgaa aaggcgggag
    12961 cgccacccgg gcggcgcagc caccgcggag tttcccccgc gcgcgtttga ccattacttc
    13021 cccttgcgct ccatcccgcg ccgccgtagc atccctcccc aatatgacta ccatcatcgg
    13081 gtctgccctg gtcaccctgc tcctgctggc cggccgcctg ccggctcaaa cccccagcac
    13141 aacggcgctg gccgcgccgc tggttccagc cacgcgcagc acccccacca actggctcgc
    13201 ccgccatgag ggctttgtcg cccaaaccca acgcggcggc attgatttgc tctttatggg
    13261 cgactccatc accgacggct ggcgcaaaaa gggcgccgcc gtctgggacc actactacgc
    13321 gccgcgccgc gccgcgaatt acggcatcgg cggcgaccgc acgcagcacg tgctctggcg
    13381 cctgcagcat ggcgagctga ccggcctcaa acccaaggtg accgtgctca tgatcggcac
    13441 caacaacagc aaagaggatt cgcccgcgga catcgccgcc gccatccagc tcatcgtgcg
    13501 ggagattcgc gctcgctgcc ccaaaaccaa agtgctcctg ctggccgtct tcccgcgtaa
    13561 tcggcccacc gacacccccg agcaactggc caccctccgc aagatcaacg aaatcatcgc
    13621 ccggctcgac gacggcaaga aggtgcgctt cctcgacatc aacaaggtct tcctcggccc
    13681 cgacggcaaa gttcccgccg acatcatgcc cgacttcctg cacccgaatg agcggggcta
    13741 ccagctctgg gccgaggcca tggagcccac cctggccaaa atgttgaaga cacgccccat
    13801 ccggaaataa tcactcgctc cgggccggcc gtttcgttcg cccccggcga aatcgctggc
    13861 tggccgcgcc cccggcggcg ctgtggcggg gccaatgaaa tcacgccgct cgccgattcc
    13921 atgacgcgcc atcgcctccc tgccgcggga ggtgatacgc gcggaacacg ggcggcgcgc
    13981 aaggaaacgc cggcattgtc ttcttgaccc ccctcggggc agccgctatg ggttgcgtta
    14041 tgacaccgcc catctcacgg cgagggttcg tgaagtcctc cctgatcgcc gccgccggcg
    14101 ttccgctggc tctgcgcggc caaaccaatc cggcgccgcc aaccccgtcc acccctggcg
    14161 ctcccgctcc gcagccgccg ttgccgacgg gccgcatcgg cggccaggaa ttcagccgcc
    14221 tgatgctcgg cggcaacctg atcggcggct gggcgcactc ccgcgacctg gcctacgtcg
    14281 ccacactcgc gcgccgctat aacaccgacg ccaaaatccg cgagaccttc gagttggccg
    14341 aagcccacgg cattaccacc atcaacacct gggtcatgca ggaaaactcg cacctcttcg
    14401 accactggaa gcggggcggt aaaatgaaat ggattgccca ggtccgcctc gatgccgggg
    14461 gcggctactc ccagatcacc cgggcgattg acgaaggagc cgcgggcgtg catatcacgg
    14521 gcgacaccgc ggaatcgttg ctcgcggcgg gccagttcga aaaggtgggt gaaaccctcg
    14581 agttgatcaa agcgaagaag cgcctcgccg gcgtcgccgc ccacgacctg cgcgtcatca
    14641 tcgaatgcga aaaagccggt ctggacgtgg acttctacca gaagactctg cattcgcatg
    14701 attactacac cgcgccccgc ccgggagaaa aggacgcgct gggcgcgaac gacaactcct
    14761 ggtgcagcga tccccaggcg gtcattgact tcatggcgac ggtcaaaaag ccctggattg
    14821 ccttcaaaat cctcgccgcc ggcgccatcc atccccgcgc ggcgtttccg tatgccttta
    14881 accacggggc ggacttcatc ctggtcggca tgttcgactg gcagatcgaa gaaaacgccc
    14941 ggctggcccg ccgggttatt gcggctgcct ccggccctgc ctccaagcgc acccgcccct
    15001 ggtacggctg agtcggggcc tgcgcggccc tgcgggcgca tctcagttta acatggcctt
    15061 ttgccggtta aagccggcaa gaacgcggtt ggaaggctaa accggaacga ttcaattggg
    15121 cagttcaacc gcccgccctc gttccatcgt tgcctctgga cgaacctgac cccccaatcc
    15181 gcagcctcgg cgaacccttt gtttggcgaa ttcaacggcg gagctactga accccgagtc
    15241 tttcgactgt aaaatcagta gtttaggtat agagagggac caaacaaagg cagcctcatg
    15301 ccgacttcgg cttaccttgg acgccccccg gccaccccct gctcccaagc cgtgtacaag
    15361 gggtgtacaa gggatgccca tcgggtgtac tagctaaaat cctatgagct cccggtgagc
    15421 atcccttgta caccccttgt acacggcctg aaggcatggt atatccaagg tgcctgcggg
    15481 ataatggata cggctgcctg gaaagaccga atcgagggga tagggcgggg ggataattac
    15541 taagttgtgt aaaaacagtt agttacgaag tcgggtctgg caaaaggcgg tttttcaagg
    15601 gcggatgggc atccgaatgc cttttggctt cgcgtatgcc ttatctgaaa cgttgccgtt
    15661 gcgcagtcca accgtgcgcc ctggcttcac tggagtcgaa gcggctctgg caggcccgcg
    15721 gatagcttca acgaaccgtt gggttggcaa gttcaacggc ttggtcgttg gctcccgaat
    15781 atccttcgcc cgtaagaacg ggaacttgcg tgccagagga gttggaatga gggcaacctt
    15841 tgaatcgctc cggctaaaaa ggcgggcatc gccggtccgg agttcgcgct cctgcccgct
    15901 gtggtggcgt tgagaatcgc ggctgcctgc ctctccgcct taaggcgtat tacttttttc
    15961 cgcgcgcaat tttagccttg cttcgggaac gtcaaaaccg caaactacga gctatgataa
    16021 cggcgcgtcg gacgggtttt cgaaccgcca gtgttagcgc gggcaggagg ccgcacggcg
    16081 tctccacgga gacgcggagg cttggggttg aggcttgtaa atcataaatt acgcatgaat
    16141 acagctcctt ttagatggtg ttcccgaaaa gccccgcagc gggcgcgcgg gttcacgctc
    16201 atcgaattac tggtcgtcat cgccatcatc gcgatcctgg cggcgctgct gctgcccgcg
    16261 ttgacgcgcg ccaagctcaa ggcccagggc gtgcagtgca tgaacaatca caagcaactg
    16321 gccctcgcat ggcggatgta tgtcgaggat agcagcgacc ggctgcccta tgccagcagt
    16381 tggccggttg gcacctggat ggccgaaacc tggtgcaacg gccggatgga ttttgccgcc
    16441 ggcaaccgct cgaattggga tcccacggtg gacattcacc gaagcccgtt gtggccttat
    16501 tgcggaaaga acctgggcat cttccactgc ccggccgacc gggcttatgt ggtggttggc
    16561 gccgagcgga agacgcggct gcgcagcatg gccatgaacg cgtatgtggg cggctttgcg
    16621 gggaaaccca ttgccaccgg aaacatggcc gcttataccg tgtacacgaa atactccgcc
    16681 atggaccggc ccggccctga ccgcatcttc gtgtttattg acgagcgtga ggacgcgatc
    16741 aactggggca actatcttca ggacatgacc ggttattcgc ccccgaaccc ccggatgtac
    16801 cggtggctgg atttgccggc cgcttaccac ggcaatgccg gcgggctttc gtttgccgat
    16861 ggccattccg aaatcaagaa atggcgtgac ggccgcacct cgcgcccgat caaggagcag
    16921 ggggccatct ttgacggctc caccccgatt ccccaggatg gcaacatgga catcggctgg
    16981 atgcaggatc gcgcgacgcg tccgaaactc taattccctt tgcaaagaat ggggccgaaa
    17041 cgcagcttcg ccgctgccgc gctgctgcta tccgccgggt tgctcgctgc gaggggagca
    17101 tttgcgggcg gcctgatttg ccttcaaagc aactcctgcc aggcccgggt tgacccctcc
    17161 cgcctggagg tcgcgctgcg caacggatcg cagccggaga tgatcgtgtc cgcggcccag
    17221 accaacctgg gccgggtggc cgacttgcgc tttgaccgcg agcttaatcc cggcacggct
    17281 tcgtggacgc tgcccgagcg caaggttacg ctgaccttca cggtgacgga aaaccgcctg
    17341 gccgttcacg tgcgggcgac ggaacccggc gaattcactt tccccgtcat tcccgccggc
    17401 ggcccggcca aagcctggat tctgccgctt tttgaagggg tgtatgcccc gtgcggcgat
    17461 acgaattggg agacctttct gaccacgcac ggcgctttga ataccaccgc ggacctcgtc
    17521 ttgccttttg tgggcctgga atgccgcgcg gcggccccgg cccgggcctg gacgctgacg
    17581 tgcatcctga ccaacccgtt caacaatcaa ctccagtttg tgcgcgcgcc ggactctacg
    17641 ctggcggcac gcctgacgca tcagttcacc cgcaaccacc cggtgaagga atacgcggtg
    17701 gtcttcgagc tgggcccccc ttctccgatc gaaccggcgc ggcggtaccg gcagtggctg
    17761 atcgagcgcg gagaatttgt ccgcttcagc gacaagattc ggaaaacgcc cgaggcggcg
    17821 aagctgccgg gcgcggcgca tgtttacctc tggggcaacg acctgctgaa ccaggcggac
    17881 gtcaccgatt ggaaaggctt tgcccgggcg ctgcaggccg gcgcggcggc gggcgcatcc
    17941 ccgccggtgc ggtggatttg ggatcagcta aagcccgaca cccggacggt ggcggcccaa
    18001 atcctcgcgg cggcctggcc ggaccgctac cagaagtcgc agatcatcga agacctcaac
    18061 cgcctgctcg agcagccgga gtttgccggc gcggcggcgg ggctggaggc cgcgctggag
    18121 ccggagattg ccgcgctcct gaagtccgac cgccgctcgc taagccgggc ggatcgcagc
    18181 cgcctgaata cgcacttgct ggcggcggca ttcccggggc tgctggccca acccgacgcc
    18241 tggggcaatg ggacttcgcc ccaaatgatc cagcagttgg cggcggcggg cctggaccgc
    18301 ctgtggctgg gcagcgatgg gtggcgcggg tttcaggaac gtccgcagac ggtcgccgca
    18361 gcgaaaaagg cgggctacct catcggccct tacgattcct accacagcat ccatcgcccg
    18421 ggcgagccgg acacctggga gaccgcgcag tttggcgcgg cgctgtatga aacgggagcc
    18481 atcgtcaatg ccgacggcac ccggcgccgg ggcttcaagc aaaagggctt cctgctgagc
    18541 cccgccgccg cgcggcccta tgtcgaaaag cgcgtcgcgg cgctcatgcc gtcgttccac
    18601 gccaactcgt ggttcataga ctgcgatggg ttcggagatt attttgacga ttatgcagag
    18661 tcgcatccgg ccacgcagca atcggatctg caggcccgcg tctcacgcct ggcgtggatt
    18721 cgcgacacgt tcggcgtggt gatcggcacc gaaggctgct ccgcgggcgt ggccgcgacg
    18781 gtgcatttcg cccacggcgt cctgacgccg gtcatcgggt ggggcgaccc ggacctgacc
    18841 gacaagcaat ccccctacta cctgggctcc tactacccgc cggacgagcc gcgggtcttt
    18901 ttccagccgg tgccgatgaa agcgaaatac cgctaccttt actacaaccc gggctttcgg
    18961 ctgccgctgt ttgaaacggt gtttcatgat tcggtgatcg ccacgcacca ctggatgttt
    19021 gccagcttga aggcgcggga ggacgctcca accgtggaac tgctggagct gctttaccag
    19081 gtgccgccgc tttaccacct gaaccgggcg gagttccaga aacgccgggc gcaaatcaaa
    19141 cggcatttcg agttcttctc cccgctgcac cgggagacgg cgctgctgcc gctggccgat
    19201 ttccaatggc tcacgcccga tcgccgggtt caacgcacgg tctttggcga ccgtttcgag
    19261 gtgattgcca acttcgggcc ggcggagttc cggcgcgatg acgcgcggct cggcccgcgc
    19321 agcctccaag tccggcatct ccttaccggc cggacgcaaa cctacacggc ccaatgaccg
    19381 cgcgccccgg gccccgggcg cggcttagct tgggctcgac agaaccggca ggccgttgac
    19441 gcgcatcccc gtccggccgc gcagaaaaac gacgcgccgg taacccgcct cggtcagcag
    19501 agtgcgccat ttgctcttca caaaattggc ctggttttcg ccggcgtaat ggatcaggac
    19561 gactacggcc aaatcgcggc ccgtctgctg ccgggcaacc gccagaacgc cgttctggtc
    19621 caatatggat aaaaacgggc cttccttcgc ttcgggcttc aagacgtaat tggtgtcgtc
    19681 ggtataataa cgaacgatga ggctggcgtg ttcctggtct ttgaatcgcg tggtgtgcgc
    19741 gccccgggga gtggagcatg ccgaggtgag gaccgccagg aacactaaca aaacgcagac
    19801 tctaatcata ccccggaggt gtgcatattt cctgccaccc gggcgtccgg gcatgattca
    19861 aattgagccc ggaaaaaatg gcaagccggg cgcccgccgc ggcggcctga aaatctccgg
    19921 aacgctggaa gcgcggaatt accccgcccg cgcaaccgtc cataaaccgg acacgggcct
    19981 taatttgagg cacgccccca gcccggcgcg gcgcggcgcg cgaaccggcg gcgggggcgt
    20041 caccgttttc agcccggagc ccgcgcggct tccgccccgg cgcttttccc attgccaccg
    20101 ccctcaaggc ggaggagaat ctccgcgtga cgggctccgc ccgcggaaaa acctatgcgc
    20161 ctttgcttgc tgttaatgac catgatctgt ttatccggct gccaatcggc cagcgttcgg
    20221 acagacgccg tctgccagag ctatacgctg gactatgaca cccccaccga tcccgcgctc
    20281 caggccgaac tcgaggcgtt ggatgcccgg ttgcgcgagc gctgcgggat gaccgccgaa
    20341 cctacggcgg tgggtgtgct ggatttgaaa cgcctgcgcc tggcgctgat caacccggac
    20401 cggattgact atgcggcaag cgtggcgaaa gtgggcattc tgctggccta cttccagttg
    20461 cacccggacg ccgctacgca cctggatccg gtggtccggc atgaactcgg gctgatggcc
    20521 aaggcgtcca gcaatgaaat ggccgccaaa tattcccggc agatgggttt gcggcaaata
    20581 caggaggtgc tcaactccta tcatttctac gatgcccgcc gcggcggcgg gctttgggtg
    20641 gggcggcatt atggcgtgga cggcgagcgc attggcgacc ccctcgccga tcactcacac
    20701 gccatgaccg tgcggcaggt gctgcgcttc tacctgctgc tcgaacaggg caagctcgtt
    20761 tcgccgcaag cctcgcagac catgcgggaa atcttcgccg cgcccgacat cccgcacgat
    20821 gatatcaagt tcgtaaaggg cctggccggg cgggatgtgc gcatcctccg gaagtggggc
    20881 agttggaaag attggctgca cgactcggcg gtcatcaccg gcccgggccg gcactacatt
    20941 ctggtggcca tgacgcatca ccccaacggc gatcaatacc tggcggactt ggccggggcg
    21001 gtggacgacc tgctggcccg ccgcccgtag tttgaaggat gagcccggcc gcacccggcg
    21061 gcgttttcca tcaaaaacat ccggggacgg cagaaaaagc tttatccccg ggggccgggg
    21121 cctgaaactg tgcggatgca actgatttca acctttcatc ggggccgcgg aggttccgcg
    21181 ttattgattt tggcgatctg ggcggcggct tgcggcggag gccagcacgc cgcgctcggc
    21241 gcgccgaaag taaaaccgcc gaaaggcttt acggccctct tcaacggcca ggacctgacc
    21301 ggctggcgcg gcggagagac gtttgacccg cgcaagctgc aggcgctgcc gccggagcaa
    21361 cgcgcggcgc agatcgccaa gtggacggaa agcatgaaga cccactggca cgccgaaaaa
    21421 ggcgagttgg tcaacgatgg ccagggggcg tatgccacca ccgaaaagga ttacggggac
    21481 ttcgagctgc tggtcgagta taagaccgtg ccccgggccg attcgggcat ttacctgcgg
    21541 ggctgccccc aggtgcagat ttgggattac accgatgcgg agaagttccc gctcggcgcg
    21601 aaccggggct ccggcggcct ctggaacaac agcccgggcg cacccggcaa ggaccccctg
    21661 gtgctggccg acaagcgatt cgggaagtgg aacgcctgcc gcatcgtcat ggtgggcgcg
    21721 cgggtgagcg tctggctgaa tggcaaatgc gtggtggatc atgcgctgat ggaaaactac
    21781 tacgaccgcc agacgccggt gccgcccacc ggcccgattg ccttgcagac gcatggcggt
    21841 gaaatccgct ggcgcaatat cttcctccgc gaaatcggcg ccgccgaggc caaccaaatc
    21901 ctggcccgcc acggcaatgc gggcttcaaa tccatcttca acgggcgcga cttccgcggc
    21961 tgggccgggc ccacgaataa ttaccaggtc gtggacggaa ccatccaatg cctgccgaag
    22021 aaaggcggca cgatctacac gacggaggaa tatcgcgatt tcgcggcgcg gctggagttc
    22081 aaactgccgc ccggcggcaa taacggcctg gccatccgct accccggcga gggcgatacc
    22141 gcctacgtgg gcatgtgcga actccaggtg ctggatgacc actacgaaca ggtcaccggc
    22201 catgcgctgg acccgcgcca ggcgcacggc tcggcctatg gcatggtcgc cgccgcgcgc
    22261 ggctaccagc gccccatcgg ggagtggaac ttccaggaaa tcaccgttca aggctcgaag
    22321 ctcaaggtcg aactgaacgg cacgctcatc ctgaactgcg acctggccgg ggtgaaggag
    22381 ttcatgggcg accgcccgca ccccggcaaa gaccggacga gcggttattt tgggttcgcg
    22441 gggcataatg acccggtggc gttccgccat atcgcgatca aaccgctttg aacgcgccgg
    22501 gcggcccggc ggttgcgcgc ttgaatccat atcacgcaac tgctatcctg agccggtgaa
    22561 tgttaatatg gttgccattc ttgcttcttc ccggatttcc cgccggctcc cggcgtgcct
    22621 gggcgccatc gcgctggcgc tgctcggcct cggcctgacc ggctgccgcc acgtcgagcc
    22681 agactcctgc gcgccgccgg ccacgaacgc accgcccgtc gtcgctccgc aaccgcccgc
    22741 cgcgaccaat gccacggcca gcaaacccgc cgagacgccc aagcggtata gctacacccc
    22801 gagggtttac ccggtggacg aacgcggctt ttcgccggtg gacaaggccc ttgccaaggc
    22861 ctatgcccgc gaggatatgg tggccgacga tgccgaggga tcactgaatc cctacgtcat
    22921 gaaggtgatt tccgcctacc cgctcgatgg ttcctacccc taccactgcg gctggacgcc
    22981 gcgcgagtat gacctctaca acggcgtgac gcaggacctc tggtataagg gcatggtcgt
    23041 cgccaaggcc taccccgacg gctcgcggtg cagctattgc tgcggcctca cgtttgagat
    23101 atttgtgcgg gcgatgaagc tgcgcaacgt gcagaagggc ctcgacccgg acgacttcaa
    23161 cggcatgacc ttcagcgatt tgttcaacct gctccagctt tggtatattg aaggccccgg
    23221 cgattgcgag caacgcggca tcacgtccta cggcctgggc cgcgccatca ccaactttga
    23281 ggaggtgcgc cccggggatt tcctcagcta cagcaccacc ccggcaggcg gtcattcagt
    23341 ggtcttcatt gactggatac gcgatgagca gaacaagatc gtggggctga agtacttttc
    23401 gtccaacctc tccggcacgc gcggcgtggg ctatggctcg ggccgattca gcgattccac
    23461 ctccaccggc cgggggctgc tgcgcaaatc cttgcgcatc gcccgcgtgg gcgccatcaa
    23521 ggactacaaa ccctttgacc gcgcggccat tccgctgcgc aacgcctacc tgcccaccca
    23581 gccgacccgc atcatctacc tgcccgcccc cgcggcggct ccggcacccg acgccgccag
    23641 gtaacgccgc ttcgcgcgcc acggaatccg cggcgcccgg cgaaagccgg cgcggcgcgg
    23701 cgggttcaga agcttagcag aagctgccag atgtacatat tgccgaaggc gtctatatcc
    23761 tcccagaccc ggcggatcaa ctcagggctg ccggtgatct cgggcaggtc tttgaggcgt
    23821 tcttcccagg gaaaacaaac gcccgggcgc acgtgcggcg cggggcgccc ctgcaggccg
    23881 gtgagctgct ggcggtccgc caccgaggac ggcagatcgc agggcggcgt ggccgtgggc
    23941 ggcgagtacg agccggcgga atagacgccg tattgccgcg ccgtatccgt catgacgcgg
    24001 atgttctcga cgcgggtatc gttctgcatg atcgccccgg catccatgat atagccgccg
    24061 tcgcgggcca cctcgctgat gacgcgccgg cagaaggcgc gcacttcctc cggctggccg
    24121 tagctgagca gcgcgttggg gatgccgccg ctcagggcaa acttgtcgtg cagcttgcga
    24181 tgggcaagga agacgtcgtc ctggtcgcaa tgaaagacaa tgctgcgatc cggcagctcg
    24241 cggaaggcgt cgaagtggtg atgccacttg ccctcggcgt agaagagcgt ctggtgcccc
    24301 tgccgccaga actcctcgat gatggggcgg agtgtgggcc agttatggga ggcgaaagtc
    24361 cggggattga tgaacggcac gcagccgcgg tgcatccaga agccaatggg cacctgccgg
    24421 gccgggtcgg cggtggtcag gcccacgtgg cagagatgcg gcatgagcgc ctcgcaagcc
    24481 tgcaggacct tttggggctg ggtgtgcatg tccatcgtca ggcccacgta gccgcgcagc
    24541 ttgtcggcga ggatgtcgaa cggcgccttg aaaatgccgg cgatggcgga aaccgtgccg
    24601 ctttcggtcc gcaggcgggc gatggcgggg ccaaaggcgt aaaaatagga aagcatggcc
    24661 atcgcgccct tcacgaagga aaggttgtgg cggtaggtgg cgggcgcgcc gggcgggctg
    24721 acttcggtgg acacgcgcgg aagccagacg ttgtagagaa aggcggtggg atcagcgatg
    24781 agcgcgtcgt attcatcggc gcgcatccag gcctgctcct ccggcggctc gatataattg
    24841 aacccggtcg tatgcgggat gccgatgccg gggatgccgt aatagcgcaa ccccaccgcc
    24901 tgcgccagcc cggtccagac ataaaccatg ttcggcacca ccgcctccca gccgaagtcc
    24961 cgcgccgtca ccagcgccgc ctcgaacgcc ttgttgtagt cgtgcgccac ttcctggcag
    25021 gtatagccgg cgtattgcgc ggtgaactcc gccacgaacg gccggagcgg cacctggtcc
    25081 ggcttttcgt tgcgcagggc ggtgatgtag cgatggagac gctgctgata gagttgctcc
    25141 gtattcatag ggccggggcg cagtttaacg gattcgcggc ggcgcgggcg agcaaaatcg
    25201 cgccgggaac gagccgggcc gccgccccgg gcggtggatt gacttggcgg cggcaaagaa
    25261 cctaacttcg gcgcatgaag atttgggacg cgcattgcca tttatcgggc gtgccggggc
    25321 aaacgccggc cgagcggatg gcgcagctcc tctcgttcgc cgaccggctg gggattgaac
    25381 ggctctgcgt gtgcatgggg atgcggtggt cttatgaccc gacgccagcg gacatgcgcc
    25441 agcaaaacga cgaggtgctc gccgcgctgc agcactggcc gcaccgggcc ctgggctacg
    25501 tctatctgac gcccaaccac gtggcggcga gcctggcgga gctggatcgg tgcgtgcggg
    25561 acggcccgat ggtgggcgtg aaactctggg tggcgcgccg cgcccatgcg ccggggctgg
    25621 accccatcgt cgagcgcgcg acggagcttc aggcgcccat cctgcagcac acctacctca
    25681 aagccaccgg caacctgccg ggcgaatcca ccccgatgga cctggccgaa ctggcgcggc
    25741 gccatcccgc ggccaccttc atcggggcgc acgcgggcgg ggatttcgag ctgggcctcc
    25801 gcgcgctgcg cgcgtgcgag aatgtgtcgg tggacctctg tgggacggac ccggccgcgg
    25861 ggatgaccga gatggcggtg cgcgagctgg gcgcgcaccg ggtgctctac ggcagcgacg
    25921 ccggcgggcg gagcttcgcc tcgcaactgg gcaaggtgct gggcgccgac atcccggccg
    25981 cggccaaaaa actgatcctg ggggaaaacc tgcggcggct gctgcggccc atcctccagc
    26041 gcaagaaaag cctatgactt ccatgcgcca gaaagacggc ggggcggacc cgggccggct
    26101 gagccggcga agcttcctgt gcaccaccac cgtcgcggcc gcggcggccg ccgccgccgg
    26161 ctggcgcgcc gaaaccgcgg acgccgcggc gccggacccc ctgatcggcg cgaccgggcc
    26221 gcgcgggctg gtggatacga acgtctggct cgggcaatgg ccgggccggc ggctgccgct
    26281 ggaggaaacg ccggcactgg tggcgcggct gcgccgccag ggcgtgcggc aagcctgggc
    26341 gggcagcttt gacggcttgt ggcacaaaga cctcggggcg gtcaatgcgc ggctggcggc
    26401 cgagtgccgc cggcaggggc gcggtttgct gacgcctttc ggcacgatca accccgcgga
    26461 cccagcctgg gaggaggact tgcgccgctg cgccgaggag caccagatgc ccggcatccg
    26521 gctgcacccc aactaccacg gctatcaact ggacgacccg gccctggccc gcctgctcga
    26581 ccgggccggc cagcgcgggt tgattgtgca attagtggcg gccatggaag acgagcgcac
    26641 gcagcatccg cggatgcggg tgccgcaggt ggacgtggcc cccctggccg cgctgctggc
    26701 gtcccgcccg gagctgcgcc tggtgttgct caactggggg cgcggggcca acagcgttca
    26761 accgccccgg ctggcggagg ccggccagat ttgcttcgat atcgccacgc tggaaggagt
    26821 gggcggagtg gccagcctgc tgcggcaggt ctccgccggg cgcgtggtct ttggctcctg
    26881 cgccccgctc ttctacctcg aatccgcgct gctcaaactc caggaatcgc cgctgaccga
    26941 agcgcaactg gccgccgtcc gcgcggacaa cgcgctgcgc ctgcggcgcc gggtctccta
    27001 acccttccgc ccggccccgc gcgtcaattt ccgtatttca cgccgcagcc atagggcttg
    27061 gtctgcgtca cgaccggccg ctctccggcc ttcagtttct gaaccgcctc gcgcacataa
    27121 ttgcgggcgg tgcgcggatc gccctccgtg gcggcccgat cgtccagcgc gccctggtag
    27181 gccaggacgc cggcttgatc aatgacgaac aggtgcggcg tggttttcat gccatacgcc
    27241 ttgccgatcc gcccggagcc atcgtccagc caggcggcgg cggcaatctt ctgcgcggtc
    27301 cattcctttt gcgcggcttc cgggcggcga tacccgggat gcttcgggga aacggagttc
    27361 acgagcagcc acaccacgcc ctgggcgccc aggtcgcgct gcaattcctg gatcgcgccg
    27421 ctgcgatatt gatggcggac ataggggcaa tcgaggttgt aggattccag caccacgatt
    27481 ttgcccttgt aatcgccgag cttgtgcgtc cggccgtgaa tgtccgtgcc cgagaagtcc
    27541 ggcgcgggct ggccaacttc cgccgccgcc aggctcaggc taaggagccc gagcccggcc
    27601 cagagtgcaa gtattcgttt cataactgcg tcaaaggatg cctgactaac cttattccgt
    27661 tccgcaataa ggaagcacaa ctttggccgg ggggcaactc gctcaccccg ctccccggcc
    27721 cgcggcgccg ttgccgcggc gcgcggccgg ctacaacgcg gcgcgcgcca gcgcgtcgag
    27781 cacgatgccc ggcgtcaacg cttcgggcag gacctgcggc ggttcggcgg gattccgggg
    27841 atacaccagc accagcggca cgcccgcgcg accgtagcgg ttcaactcgg cggtaatgtc
    27901 atccggcagc cgggtgtaat cgccgagcag ggcgaccgcg ttgatctgct tgagccgggc
    27961 gcgcaccgag gggatgtcaa tggccacgcg cagattggcc tggcaggtca ggcaccagtc
    28021 ggcggtgaaa tccaccagca cgggccggcc ctgggcgcgc gccgcggcca gcgccgccgg
    28081 gctccagcgc tgccattcga tgccccccgg gaggcccggg ttctccgcgg aagcgtccgc
    28141 gaccggcgcg cgccagcgca actgcccttc caccgcgtag agaaatcccc cggccaggag
    28201 aaccagcgcc accgccagcc ccagcccgcg ccgcgtgcgc ccgcgctgga agaattcgcc
    28261 atagacccag gccgccagcg ccagcacgac caggaaaagc cccagccaca gcgcgcgccg
    28321 gccatagtgc agcgggatga gactgaagag ccacaccgcc gtcgccagca tcgggaagcc
    28381 catcgccacc ttgaaccgct ccatccacgc gcccggtttg ggcaggaact tcaaccacgc
    28441 cggatgccag ctcaacacca cgtaaggcgc ggccaggccc aggcccacca ccagaaacat
    28501 cacaatgatg aggcccgccg gctggctgaa cgcaaatccc agggccgccc cgaggaacgg
    28561 cgcggtgcag ggagtcgcca gaatcgtcgc cagcacgccg ttgaaaaacg cgcccgccgg
    28621 gccgcctttc gcggccaggg cgcccgccgc ccccaacacc cgcccgccgg ggttgatctc
    28681 gaacaggcca aaaaggttca gcgccaccag ggtcaccagc atggtcagca gcaccagaaa
    28741 ctgcggattg ccgaactgca ttccccagcc cgctttatgc cccgccgcct tgaccccgat
    28801 caccagtccg gccagcacga gaaacgacac cagcaccccc agcgcgtaga ttaatcccaa
    28861 ctggcgaacc cggcgcggct catcccgcgc ctgccccaca aaaccgagaa tcttcaaggc
    28921 gatgaccggc agcacgcatg gcatcacatt caaaatcagc ccgcccagaa acgcgtacag
    28981 cagcatcttc caaagcgcgg gagtccccag gccggccccg gcggacggag cgcgcccgtc
    29041 ccgggcgtcg gactccgaat cgcggatcgg caggcgaacc tcatcggccc ggcgcgcggc
    29101 gcccgcttcc tgaaccagca gcccggagac gtgccgcggc cagtctccgc gcagcttcgt
    29161 gaccttcttg cgcaggcgaa ggattcccgg gtcggactcg gctttctccg tcgccggctg
    29221 cacctcgaac tcctcgctgg cgtcggggaa gaaatccgcc tcggtgacac cggcggcggg
    29281 cttccattcc agcatcaggg tgcgggaatt gccgacgggg acggaatccc accacgcgcg
    29341 gaccgacaat ccaccgccgc gctgcggaag tttgccctgc caggctttaa gccgcgcgga
    29401 ctctttcgac gccttggttt ccgcgcccac aatgagcgtg gcccgcaccc tggccttgcc
    29461 gggaacacag gccacctcgc actccagcca ggagacttcg gcgctcaact ccagcgggcc
    29521 ggtccgcaaa tccgcggcca gccgcagcgg aaccaacaac acaacttcat gggtgtaaac
    29581 ataagtgacc aggtccgatt ccaggtattt ttccggaacc ggccattgga tgtccccggc
    29641 ggccacgccc gccggcaact gccattcaac ggcggttgcc atgccggact cgcccgggtt
    29701 ttgccaatag gtatgccagt gcgggtccat gcgaagatga atccccgcca aaacggtatc
    29761 ccccgggcgg gcaacctccg catccagcaa caactgcgcc tgcgtatgag ccgcccgggc
    29821 agacagcccg cccaggccaa gccagaatac gccgaacaaa ctcaggaatc gtcgcccaaa
    29881 catcgaatac attgattggc gacaagttag agataaatcc gcgcaaatgc aaatgcgctc
    29941 cgccacaacc gatggctatt tccaatcaac tcaagcgcat tgctgcctct gcatcgttaa
    30001 cggccccgaa tttaagcaaa taacggattg tttcgcgccc aaccgggcgt ttttgaaggg
    30061 atttaaaccg gaacgactca gttggacagc ccaaccgtcc tctcttactc catcggatcc
    30121 tgatcaacct tgaccgccca gaccatagcc tcagcgatat tttgtttagc gaatccaata
    30181 gcatggccgc cggactccgt gtctttccac gataaaacca atggtttagg cttgcgccta
    30241 aaaagaggca aaattgaggc cgaatctgaa tcatgccagc ttcggcttat cctggaccta
    30301 ctctggacgc accctgaacg ccccctatct ctacgccgtg tacaacgggt gtacaaggga
    30361 tgctcatcgg atgcaccagc taaaatccga tggactcccg gtgagcatct cttgtacacc
    30421 cattgtacac ggcgtagaag cagggtaaat ccagggtatt gggatagtgg agctggatgc
    30481 ctggaaaggc cgaaatgatg aaatggaacg ggaggacaaa tactaagttc agtaaaaaca
    30541 gttagttata agtccgaagc tgatgggaga tgattttcca aaggcgggtg ggcgctcgga
//
LOCUS       DAOPQG010000035        30115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1978_length_30115_cov_15.953460, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000035 DAOPQG010000000
VERSION     DAOPQG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 30115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30115
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_30115_cov_15.953460"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PKI07_05735"
     CDS             complement(<1..857)
                     /locus_tag="PKI07_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50588.1"
                     /translation="MLTAWLIFSALCSCAGWVLSAVHQLNAVGYGGVFLASLAVAWVL
                     RKRLFPGKFRGWSLRKARRRFRRSFPLAFLLLALLAILGGLIHAPSNYDALAYRVPRV
                     LHWLADGQWHWIHTDFQRLNTRACGIEWLSAPLMVFTQTDRWLFIINAASFCLLPGLV
                     FSLFTRVGVRPRVAWHWMWLLPTGYCYLLQAGSIANDMFSTMYALAAIDLALRARKSG
                     KASEVCLSVLAAALLTGTKATNLPLLLPWAIAFVPTWRIWFMRPLPVAVAILFGLGAS
                     LVPISVLNA"
     gene            complement(1229..1648)
                     /locus_tag="PKI07_05740"
     CDS             complement(1229..1648)
                     /locus_tag="PKI07_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOC50589.1"
                     /translation="MKSLTEHLWFETPNRRDYLNITNTVEALVRRSGVKEGLCLVNAM
                     HITASVYINDAEDGLLHDYDVWLEKLAPHAPTSQYHHNRTGEDNADAHMKRQIMGREV
                     VVAITKGKLDLGPWEQIFYGEFDGGRRKRVLVKIIGE"
     gene            complement(1780..2763)
                     /locus_tag="PKI07_05745"
     CDS             complement(1780..2763)
                     /locus_tag="PKI07_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC50590.1"
                     /translation="MTTPIIEVNNLTKVFRTYKKQPGFAGAVRGLFRRKYEPTLAVRD
                     VSFAIQPGELVGFLGPNGAGKTTTLKMLAGLLYPTSGSARVLGYVPWQRHDGYRRQFA
                     LLLGQKNQLWWDLPARESLELNARIYGISAATLERTVAEMSELLAVRDKLNVSVRELS
                     LGERTKMELIASLLHQPKVLFLDEPTIGLDVVSQKIVREFLRRHNARQKTTILLTSHY
                     MDDIQELCERVIIIDHGTIFFDGQLGDVVDRFADSKLITIQCQGGSQCAGEQLRPYGE
                     IVAHAPGSFQLKVKRDRVIAVCKALLDELPISDIDIQEEPIEDVIRRIFAR"
     gene            complement(2851..4191)
                     /gene="dnaA"
                     /locus_tag="PKI07_05750"
     CDS             complement(2851..4191)
                     /gene="dnaA"
                     /locus_tag="PKI07_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOC50591.1"
                     /translation="MARAQLRTMLSADQFNLWFEPLRVCSQENGTLTLEVANDFCEVW
                     LKENYLDLLQDTVAAASGQQLQINFKVAGTSGSEAPGLSSASTAATKKKVAELALDRG
                     QGNLELKVNPKNTFDTFVVGSNNNFAYAAAQAVAQTPGKAYNPLFLYGGVGLGKTHLL
                     HAIGQHVTQHKKGARVVYLSSERFTNEYIEGIQNNQLARFRKKYRQTDVLLIDDIQFL
                     AGKERIQEEFFHTFNALHEAHKQIVLTCDRPASEIQNLEHRLVSRFEWGLVTDLQPPD
                     VEMRLAILQKKAQLMSVQLPEEIMQFMANRIRSNIRRLEGALVRVASYARLTGKPLSL
                     DTVEGLLREILAEEGLGSISIEIIQKKVAEYYDIRLADMTSRRRPESIAFPRQVAMYL
                     ARQLTDASLSAIGEDFGGRDHGTVLHACRLVKDRMEVDAKVRQVVGFLEKQLMR"
     gene            complement(4623..4961)
                     /locus_tag="PKI07_05755"
     CDS             complement(4623..4961)
                     /locus_tag="PKI07_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOC50592.1"
                     /translation="MKKIEAIIKPFKLESVKEALSGLGVEGMTVLEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKIKIEVVLPDGQTAAAVEAIVKAAKTGKIGDGKVFVSNIENAIRI
                     RTEETGERAV"
     gene            complement(5237..6481)
                     /locus_tag="PKI07_05760"
     CDS             complement(5237..6481)
                     /locus_tag="PKI07_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50593.1"
                     /translation="MTTPQTPHGTDNATPFTRRRFLAGTGASVLAFTVLKPGLARAAG
                     ANAKINLGLIGCGKRGKWIAELFEQHGGYHLVAVADYFQERVDAVGEQLQVGAAQRFT
                     GLQGYRRLLEQQLDAVVIESPPYFHPEQAAAAVEAGKHVYMAKPVAVDVPGCLSIDAS
                     GRAATAKQRCFLVDFQTRANPLYQAAVQAVRAGEIGKLFFAEASYHCGPTWDSMDQLL
                     RQDPKNPEVRLRAWGVDRVLSGDVITEQNIHALDVACWMLGAEPLRACGTGGRKREFV
                     GNCWDHFAVIYHFPNDLVLSFNSRQSGYGYDDILCRVYGSQGTADTHYFGNVTVKAKE
                     FHSDGKVGNLYHDGVVANIATFHDCITRGDWTNPTVTPSVRSNLTTILGRMASYRNGT
                     VTWPEMMQAKEKLEADFKGLKA"
     gene            6992..7507
                     /locus_tag="PKI07_05765"
     CDS             6992..7507
                     /locus_tag="PKI07_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC50594.1"
                     /translation="MPIYEYACPKCRVIFNFLSKRINPDRSPVCPRCGNRNLSKQISR
                     FAAVRGLKEPTPKTDAPTDESPIPDMDDPRVERAMLEMERDMEHLDENNPRHMAHMMR
                     KMKDFMPAGSVPKELDVAIKRLEAGEDPEKIEEDMGDVLDGFLGGPDEEGGMGGGPGG
                     GYTRDSGLYDY"
     gene            7769..8971
                     /locus_tag="PKI07_05770"
     CDS             7769..8971
                     /locus_tag="PKI07_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC50595.1"
                     /translation="MFSRRLKAGYFTLEGLNSFATVYYFYYFYFFMQTEFGFGNKANL
                     ALAALNGVVYAVMAWQGGKFAQRYGYYLALKLGFGLMIGALAIGSQFHSPGVHIAVMC
                     VAVSGMCFTWPTLEALVSEGESPAGVQRMVGIYNLIWSGTGALAYFIGGTMFEKLGPR
                     SMFYVPALMVFGQLLLTLWLERLARQTPTATTPPARPPEPAPEAEAPPPAVARSRTFL
                     RLAWLANPFAYIAINTLIAVIPDVARRLGLSPMIAGYCCSLWCFSRVGTFLLLWRWSG
                     WHYRFRWLLAAYLALTGTFAAIVMAPGLALLILAQLAFGAAAGLIYYSSLFYSMDLGD
                     TKGEHGGIHEAAIGLGNFVGPAVGALSLHYFSHYAQSGTLAVSGLLFLGLGGLLVIWR
                     NGRAGGTN"
     gene            9054..9824
                     /locus_tag="PKI07_05775"
     CDS             9054..9824
                     /locus_tag="PKI07_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOC50596.1"
                     /translation="MSLLKGKLGIVFGVANRRSIAWAIAQVWHGAGAKLAFTYQGERL
                     KDNVEELAGTFGGDTLILPCDVTRDEEIARVFQQVGDRFGQLHLLLHSVAFAPKDALE
                     GQFLNTSRAAFRIALDVSAYSLVALARAAAPLMTAGGSIIGMSYYGAEKVVPHYNVMG
                     VAKAALEASTRYLAYDLGPRRIRVNCISAGPVNTLAARGIGGFSEMLRHYEAHSPLQR
                     NVLPEELGAAGLFLASDGAAAITGQVLYVDCGYQIMGM"
     gene            complement(9949..10200)
                     /locus_tag="PKI07_05780"
     CDS             complement(9949..10200)
                     /locus_tag="PKI07_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50597.1"
                     /translation="MAAPPPPSPRTPARPMAVQEAIEEVTRIIATLRATLEDMDEVLE
                     TLELAERQKIADEQEIETLRRGLRQLQRPREDGASPARR"
     gene            10647..11684
                     /locus_tag="PKI07_05785"
     CDS             10647..11684
                     /locus_tag="PKI07_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOC50598.1"
                     /translation="MKLNFKRLNLQLTHLWAIARGGGSTTAQTVMVELTGADGTVGWG
                     EAAPIARYRESLDTVEAFLKRVDPRGLSFKDVEGSMVYLDTLSPHDMSAKCALNIALL
                     DGAAKLANKPIYDYLGLGFRENHHVSSFTIGIDKPDVIRKKVQEAAPYPVLKMKVGVA
                     DDKINVKALREIAPTKTVRVDANEGWKTKEHALEMIEWLAQDGHIEFIEQPMPSATPV
                     KDWVWLKERSPLPIMGDESYHTIKDLAQARECFHAVNVKLAKSAGISGGFEALSAARK
                     AGLKTMLGCMIESSILISAAAHLAELCDYLDLDGNILISNDPYVGATAEKGIMSFAKA
                     PAKYGLRVSAR"
     gene            complement(11704..12003)
                     /locus_tag="PKI07_05790"
     CDS             complement(11704..12003)
                     /locus_tag="PKI07_05790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOC50599.1"
                     /translation="MNNQSLTVIAQIRAQPGRESAVRQELLALLVPSRADAGCLNYDL
                     HQGLDNPALFLFHETWASPAHLEAHLRKPDLQAALARIGPWLAAPPQVTRWEKIG"
     gene            12423..13391
                     /locus_tag="PKI07_05795"
     CDS             12423..13391
                     /locus_tag="PKI07_05795"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015079698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AccI family restriction endonuclease"
                     /protein_id="HOC50600.1"
                     /translation="MARHSEHPFEKVLAVSTEDIRMALLSMGNAPWAEFLLNPRRLRG
                     SDFLMRWSQGIWSEERLTQAVNATGRYFALPYGPSGTAPDNDVRAFELYFERLEKAGL
                     GNIKRPDLLIYRTRDKARIDSAVKVLNGISELPFTPEDDPNMQVLLEHAIIAVECENS
                     LWRAKQMPDYTSPFTPQKRLGGHYGLKKTAVVPTIIIKEEDREPLKSWQKKRRVPIHV
                     WHAFFDEAFGISLVNAEKLFAQGNIEPTEQVFQAPGGATTRKIIYKIYYRHAYPLATT
                     IEEPQLVADSITDKNGHILPYVRFVGGKSQLSEQALNVLDSTKQNG"
     gene            13388..14917
                     /locus_tag="PKI07_05800"
     CDS             13388..14917
                     /locus_tag="PKI07_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOC50601.1"
                     /translation="MKQRRHQALPRTDRERETLRTKGQFWTPDWVAEFMVAYALLDQP
                     KQLLDPALGEGVFFRAAKRYAELHGFDLALFGRDIDPDILTQARQSGLKDTDLRNVEI
                     RDFVLDPPSEKYPAIVANPPYIRHHRLSALQKASLRDFARTTTGRNIDGRAGLHVYFL
                     IRALNTLLPGGRLCYIAPADICEGVFAQALWQWITSQYKLDAVIAFSADATPFPGVDT
                     NALVFCLRNESPAKHFDWVKCQRQNSRELISLLSGDKHKRYEMIEVHRRTVAEAVATG
                     LSRPPSQAMPNRYILGDFASVMRGIVTGDNDFFFMTLARAKEQGIPESLLVKAVGRIR
                     DIEGEQFTLEDIDRLEARNRPTRLLCVNGLAFEQLPITVQAYLKEGENRGLDKKALIR
                     TRKPWYRMETRKSPPIMFAYLGRRNVRFIRNHAGVVPLTCLLCVYPKREDSDFIERLW
                     KVLAHPKTIANLRKVGKSYGGDAIKVEPRSLERLPLSDHLVQAEGIEKYVQPKQSTLF
                     D"
     gene            complement(15143..16195)
                     /locus_tag="PKI07_05805"
     CDS             complement(15143..16195)
                     /locus_tag="PKI07_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOC50602.1"
                     /translation="MRCINRLWGILALAAAAGLSGCAPRALQVTTGESVVLAGQAPAT
                     LISDPSPARAVVVRNTYRDGLARTIHYEAGRDYRLEAPNRIRRTPESRIPDFGTNVLY
                     GREDFRHEQFPGFGNGAFFVYVDYAYAGRRERPRGAAAFGAARLPATRRKLRAGQPVR
                     MVAFGDSITAGGDASEPGLIFWERWADALRAKYPRARIETRNGATGGDTTAQGLQRLE
                     EKVLRQQPDLVLIGFGMNDHNREGFGVPLPAFAANLRSMVRRIRAETGAEVILFSAFP
                     PNPKWHYGSHNMAAYAAATERVARGERCAFADVYHCWQRAAARKKPEDLLGNNINHPN
                     DFGHAIYLQAFEALGL"
     gene            16294..19032
                     /gene="alaS"
                     /locus_tag="PKI07_05810"
     CDS             16294..19032
                     /gene="alaS"
                     /locus_tag="PKI07_05810"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOC50603.1"
                     /translation="MTSAEIRQSFLDFFQSREHTIVPSASLLPDSPNLLFTNAGMNPF
                     VPIFLGQRAPDVSKWAGARPGRDTRAADTQKCIRAGGKHNDLEDVGLDTYHHTFFEML
                     GNWSFGDYFKREAIDWAWELITEVWKFPPPRLYATVYAPDPARGDPAEFDQEARDAWA
                     ARFRAAGLDPQVHIVNGNRKDNFWMMGDTGPCGPCSELHMDLTPRGDSQGRLVNQGDA
                     RCIEIWNLVFIQFNANPDGTFSPLPARHVDTGMGFERVAGIIQNTRNFTDFNRIISNY
                     DTDLFRPLFDQLERLSGKKYHSTLPAPGAPALSETQQADIAFRVIADHVRTLSFAIAD
                     GIEPGNTDRNYVLRRILRRAVRYGRALGFRQPFFAPLVAALAESMGSAFPELRARQER
                     ITQVIRREEETFNKTLDRGLALFEEEVAARAADASAARAPLSGAFAFRLYDEQGFPLD
                     LTELMARERGLTVDVAGFHQLMEAQKARARAAQKKQVIELSRITTTTPTRFVGYDQWE
                     TAATVREVVGVKDKTAVILDCTPLYAEMGGQVGDTGQLERGGETWRVADTQKAGDAWL
                     HYLAGRDAPPAGAAVRVAVDRERRAAIQRHHTATHLLHWALHEVVSPDAVQKGSFVGP
                     EKLTFDFNGAPLTPRQVADLEQAVNERILENAPVTWTEARYQDLRNRPDIRQCFGEKY
                     GDVVRVLQVGGQPGALNGYSMELCGGTHVRATGEIGLFRITAESAIAAGIRRIEALAG
                     LEACRRTSEERQLLRAIADKVNSPLGELEKKIEALLAQQRALEKQLLALTQERAAQTA
                     RNLLSQAQTLNGTPALIARFDADGEQLQGIANELKGQFQGVIILGGVAQGAVSLVAAV
                     APAYAAKYPAGKIIQTLAPLVGGKGGGRPDNARGGGKDTSKLDEALARARSLLSGA"
     gene            19777..20859
                     /gene="recF"
                     /locus_tag="PKI07_05815"
     CDS             19777..20859
                     /gene="recF"
                     /locus_tag="PKI07_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOC50604.1"
                     /translation="MHLAHLRLRDFRNYARLDVDFAPGLHLLLGDNAQGKTNILEAIY
                     LMATLRSFRGVGGAQMIRHGQRGYFVGGKVIGQGEHNIKMYWSAAERNLSLNGRPVRK
                     LTDYLGALRTVIFCTEDLQLVKGAGRARRRFLDLLLSQTYPGYLPLLQRYARALRARN
                     ALLKSAGADPMALDGFSSELIKAGDEVMRRRRELVPKLSPLARLAYRRISNDAEELWL
                     DYRPSVKRDFALELAQSRSRERVYRATLVGPHRDELELRLNDRPAAAFGSEGQKRTLA
                     IALKLAQAEYLTGLHGAPPILLIDDIMGELDAKRRGGFLPLLDRAEQSRGQVFMTATE
                     ANWPVETGRTARRWLVKEGTLLSLPA"
     gene            20989..21735
                     /locus_tag="PKI07_05820"
     CDS             20989..21735
                     /locus_tag="PKI07_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOC50605.1"
                     /translation="MRDSRWNRKVNAPGGRPGLKGGGGVLVLCLLWLAALPLLAESYY
                     LPDGRPDGVALLAPPPLPGSAEETADLQTVRTVFQGRTEAEKEHAFKSASLSIFLYAP
                     AIGPFFQPGKFPKVEALFQKVRKDISAPLDRTKKHWKRRRPYELDPQLALGRPETTFS
                     YPSGHSTRGTVQALLLAELFPKQREAILAIGRQIGWDRVLIGKHFPTDIHAARTLGQA
                     IAREFLASPAFQRDLAEAQAEVRACPEANL"
     gene            21828..22385
                     /gene="efp"
                     /locus_tag="PKI07_05825"
     CDS             21828..22385
                     /gene="efp"
                     /locus_tag="PKI07_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOC50606.1"
                     /translation="MASANDLRRGMAINYNGDICVVLDTQHRTPGNLRAFVQASLRSI
                     RSGRSADVRFSSTEKIEVVPMMTRKMDFSYKDGQDYVFSDPETYETVMLTPELIGEAR
                     NFLVENGQVTVTFVEGKAVAIELPSSVVLTVADAPEGIRGDSANNVQKSVTLETGITI
                     QAPLFIKTGEKIKVDTRTGKYMERA"
     gene            complement(22473..23522)
                     /locus_tag="PKI07_05830"
     CDS             complement(22473..23522)
                     /locus_tag="PKI07_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOC50607.1"
                     /translation="MKKKSAKIRSGLLAGGNWIMDQVKMIDVYPQPEQLANIRSQSQG
                     TGGAPFNVLVDLARSGTPFPLYAAGLVGRDPLGKEILEICRHYKIDVRHLGATPKAPT
                     SYTDVMTELGSGRRTFFHARGANALWDGADLDFQKLNVRIFHLGYLLLLDALDKPDAR
                     LGTQAARLLARAQAAGVRTSVDVVSEDSDRFARIVNPALKHVDYCILNEIEAGKTTGF
                     KIRLPDGKLDTVALRHAAGALLQQGVRELVVVHFPEGAFARTRKGDDFWQPALKLPPK
                     YIAGTAGAGDAFCAGVLYGLHESWDLQRCLLTGVCIAAASLADATCTTGVKALNTSLA
                     LARKFGFGAPLDGAD"
     gene            23852..25915
                     /locus_tag="PKI07_05835"
     CDS             23852..25915
                     /locus_tag="PKI07_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50608.1"
                     /translation="MQLTRLTSGGGGLALGLSLLLGALPAGAAPPRLTPLSQGQWPAY
                     ARGAAEDVKMAGSYACLALGTSGLAVIDVSNPAHPVRTGGCDTDGQAGGLAVAGNYVY
                     VADGEAGLEIISISNPAHPVRLGGFNTSGQAKDVVVAGNLAYVADGTAGLQVIDVSNP
                     ASPVWLGGYDTSGYANGVALAGNYVYVADGPAGLQVINVSDPAHPVRTGGYDTSGYAN
                     GVAVAGGIAYVADGEAGLVIISVSNPASPSLLGSYLTWGNANRVAVAGGAAYVADILT
                     SLQIINVSNPANPMPLGFYDTGGGAAGVALAGSTACVVDAAAGLQVLNVSNPADPVRL
                     GGFNPGGTAYGVAVAGSLAYVADGAAGLQIIDVSDPLALARRGGFQTNGLAQSVAVAG
                     TTAYLACSDSGLQIINVSNPDSPARLGGYLCSYANGVAVAGSVAYVADGPAGLEIINV
                     SNPASPVRLGGYYSANWVAQGVALAGTTACVAYGEAGLQLINVSNPANPVRLGGYNTS
                     GTAYGVAVAGAVAYVADGEAGLQIINVSNPDSPVRLGGCGTGGIARGVAVAGARAYVA
                     DDEAGLQVIDVSNPASPVRLGSYPTRGTARGVAVAGNRVYVANDEKGLRVFCSLPNVQ
                     YMLRVEGGALGTPFTLEAASLLKAATNWQALFTTNPAAVPFEFTDTDVRYATQPRKFY
                     RVRQP"
     gene            complement(25955..26428)
                     /locus_tag="PKI07_05840"
     CDS             complement(25955..26428)
                     /locus_tag="PKI07_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50609.1"
                     /translation="MSPSLAMEILTYAYENDKPIYRATLNAVAEARKLRPVYFERQPK
                     PQRHAAMLVSLARPALDAVTSGLIRAWLLKKHKAMLTDFLDALGIKHEDGVVDDLPPA
                     MDDAKLKAAVETLLAKYPPETVAVYLHSFNEMNEQEWPNLATLLNADARLQLGAA"
     gene            complement(26521..27369)
                     /gene="lpxI"
                     /locus_tag="PKI07_05845"
     CDS             complement(26521..27369)
                     /gene="lpxI"
                     /locus_tag="PKI07_05845"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003303.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOC50610.1"
                     /translation="MTRLPDTLGIIAGNRSLPLLLAREARRQGVRRLVAVAVEGETDP
                     ALAALVDEIVWLKVGQLSRMIAAFTQRGVRHCVMAGQVAPRNLFDLRPDLRAMRLLLR
                     LREKNAHTIFGAIAEELQRDGVELIEATPWLQPLMPGEGFHLGPRLSDAQRADVEYGF
                     RIAKEVSRLEIGQIVLVKGGAVLAVEAFEGTDACLARGGALAGPDGGAVAVKVAKERH
                     DFRFDIPCLGPQTLETCAAARVAVLAFEAGRSLLLEQETCERLARAHKISVATIGAAP
                     RPPCQN"
     gene            complement(27563..29290)
                     /locus_tag="PKI07_05850"
     CDS             complement(27563..29290)
                     /locus_tag="PKI07_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HOC50611.1"
                     /translation="MSILIAMMKRLLAGLMLLAGGGVGAVRAAGPGDEVIIVYNTRVP
                     ESREVASYYAERRQVPASQIYGFPLSTNEEMSRVEYRSALAEPLARKLKEQKRWEIGP
                     CIISATTNQPGRVEWKPLTSAIRYAVLCYGVPLRIQRDPHLKEEGMAGLRPELRRDEA
                     AVDNELALLPMTVEKLPLFGPLRNPVYTTTNAATLSPTNGVLMVARLDGPTAAIARGL
                     VDKALQAEAEGLWGRAYFDLRNISDPGYRVGDEWIRASAGVCKQLGLETVVDENARTF
                     AAGFPLSQVAYYLGWYDEHVSGPFAQPEVEFMPGAFAYHLHSNSAGTLRAAHRHWVGP
                     LLAKGVTITMGTVCEPYLSGTPDLAAFTARLVYLGFTFGEAAYAAQSVLSWQTTVVGD
                     PLYRPFGMDPDRRHRDLEARGSKLIEWSWLRLANLNLAVGKPVREVVALLEQLEATRQ
                     SAVLTEKLGDLYLAQGKPSSAVHAYRQALPLASSPQQKIRLLLTIGERLSALGRTEEA
                     YADYQQLLQDFPNYPEPLALYQKLRPLALKLGKQAEAENYQAEINRLSPPPPPAPASP
                     PAESKPAKP"
     gene            complement(29308..30108)
                     /locus_tag="PKI07_05855"
     CDS             complement(29308..30108)
                     /locus_tag="PKI07_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC50612.1"
                     /translation="MRGTMSFTFVFLGSGTSQGVPVIGKEYPPAFLANPRNHRTRPSL
                     YVATEQTRLVIDTTPEFRLQMLREKIAWLDAVLITHSHADHIMGLDDCRRFCDLRGGQ
                     ALPVYASEATMNDLKRVFRYAFENGPRPRGYFTPEPHVVTGPFTLGDLEVTPLSLPHG
                     RTTTYGYLFVQRGRKRLAYMSDCKEVPAEVVAQVRGAEAVVLDALRPRPHLTHMCLDE
                     ALAAARRIGAGRTYFTHLTHDYDHDAAQAELPPGVELAYDGLRVAGGA"
BASE COUNT         5670 a         9792 c         9298 g         5355 t
ORIGIN      
        1 ttagcattga gcactgaaat cggcaccaga gacgctccca aaccgaacag gatggcaacc
       61 gccactggca gtggacgcat gaaccatatt cgccacgtcg gcacgaaggc aattgcccag
      121 ggcaacagca gaggcaggtt ggtggctttg gttccggtca gcagtgctgc ggcgagcacc
      181 gacaggcaga cttccgaggc tttcccggat ttccgcgccc gcagggcaag gtctatcgcc
      241 gccaaggcat acatcgtgct gaacatgtcg ttggcaatgc tgcccgcctg caagaggtag
      301 caataccccg tgggcagcag ccacatccag tgccaagcca cgcgaggtct caccccaacc
      361 cgcgtgaaca agctgaatac cagccccggc aaaaggcaaa aggatgccgc attgattata
      421 aacagccagc ggtctgtttg ggtgaagacc atcaagggcg ccgagagcca ttcgatgccg
      481 caagcgcggg tgttcaggcg ttgaaagtcg gtatgaatcc aatgccattg cccatccgcc
      541 agccagtgca atacgcgcgg gacccgatat gccagcgcgt catagttgct gggggcgtga
      601 attaggccac ccaaaatcgc cagcagcgcc agaagcagaa aggccagcgg aaacgaccgg
      661 cgaaagcgcc gccgcgcttt tcgcaaactc cagccacgga atttccccgg aaacaagcgc
      721 ttccgcaaca cccacgccac ggccaggctc gccaaaaaca ccccgccata gccgacggcg
      781 ttgagttgat gaacggcgga taaaacccag ccggcacaac tgcatagcgc gctgaagata
      841 agccaggccg tcagcattat cggaacgtgc gtgcttccgg ttgctaccgg cgaaggctgc
      901 ctggcagcac ccttggcctc aggcggtgcc tgctggactg cgtcgttcac aatactttgt
      961 caccacaaac tgacgcctca ggctagcggg gcgtttattc gaggtcaact ttaatgaaaa
     1021 ccgccggcgc aaagtgcggt gacgatcgat gcgattgcga atgctcgaac tgttcggctc
     1081 accctggcgt tggccgccac gaaaagccgc caacccacgg cgtacctggc ccgcccccat
     1141 gccggttgcc cctgccactt gctcccggct gccgcgagcg caaagcggaa ggcgttggca
     1201 agcacggcgc aggccgcacg cgcggggttt attcgccgat gatctttacg agcacacgtt
     1261 ttcggcggcc gccatcgaac tcgccgtaga agatttgctc ccacgggccg aggtcgagct
     1321 tgcctttggt gatcgccacc acaacttcgc gccccataat ctggcgtttc atgtgggcat
     1381 cggcattgtc ctcgccggtg cggttgtggt ggtattggga ggtgggcgcg tgcggcgcca
     1441 gcttttcgag ccacacgtcg tagtcatgca gcaggccgtc ttcggcgtcg ttgatataaa
     1501 cgctggcggt aatgtgcatg gcgttgacga ggcataaacc ctctttcaca ccgctgcgac
     1561 ggaccagtgc ttcgacggtg ttggtaatgt taaggtagtc gcgccggttc ggagtctcga
     1621 accacagatg ctcggtgagc gatttcatag cccccccagt ttgccagagt tccggcgtct
     1681 ggccagacgg aaccgcgggg ccggcaaatc cccccgcgcc gccgggatgc cgggaggccg
     1741 ccgccccgac gccgagacgc tttcgccccg cgcaagcgct cagcgcgcga aaatacgtcg
     1801 gatgacatcc tcgatgggct cttcttgaat atcaatgtcg ctgatcggca gttcgtcaag
     1861 cagggctttg caaaccgcga ttacgcgatc gcgcttgacc ttgagctgga agctgcccgg
     1921 cgcatgggcc acaatttcgc cgtaggggcg cagttgctcg cctgcacatt ggctgccccc
     1981 ctggcattga atagtgatca gcttggaatc ggcgaatcgg tccaccacgt cgccgagttg
     2041 gccatcaaag aaaatcgtgc cgtgatcaat gataatcacc cgctcgcaaa gctcctgaat
     2101 gtcatccatg taatggctgg tcagcaggat ggtcgtcttt tgccgggcat tgtgccggcg
     2161 cagaaattcg cggacgatct tctgcgagac aacgtccagg ccaatggtgg gctcgtccag
     2221 gaacagcacc ttgggctggt gcagcaagga tgcgatcagt tccattttcg tgcgctcgcc
     2281 cagcgaaagt tcgcgcacgc tcacattgag cttatcgcgc acggccagca gctcgctcat
     2341 ttcggcaacc gtgcgctcca gggtggctgc ggagatgccg taaatccggg cgttcaattc
     2401 gagcgattcc cgggcgggca ggtcccacca aagctggttc ttctgcccca gcaacagcgc
     2461 gaattgccgg cggtaaccgt catggcgctg ccacggcaca taccccagca cccgcgcgga
     2521 cccgctggtg gggtagagca gcccggcaag cattttcagg gtggtggtct tgcccgctcc
     2581 gttggggccg aggaacccca ccagttcccc cggttggatg gcaaaactca cgtcccgaac
     2641 ggccagcgtc ggttcatact tccgccgaaa cagaccccgc accgcgccgg caaagcctgg
     2701 ttgctttttg taggtgcgaa aaaccttggt gaggttgttt acctcgatga tcggcgtcgt
     2761 catgaatccg gcggcactgt gatcgtgtct cctcccgcgg accgtggccg gtgctgactg
     2821 cgcgccgcca ccccatttcc cggggaaacc tcagcgcata agctgctttt ccaggaagcc
     2881 gaccacctgg cgaaccttgg catctacttc catgcgatct ttgaccaacc ggcaggcgtg
     2941 cagcacggtc ccatgatcgc gcccgccgaa atcctcgccg atcgcactca gggaagcatc
     3001 cgtgagctgg cgcgccaaat acatggccac ctgccgcgga aacgcgatgc tctccggtcg
     3061 gcgccggctg gtcatgtccg ccaggcgaat atcataatac tcggccactt tcttctggat
     3121 gatttcgatg ctaatcgagc ccaggccttc ctcggcgaga atctcgcgca acaggccctc
     3181 caccgtatcg aggctcagtg gcttgccggt cagccgtgcg tacgacgcca cgcgcaccag
     3241 cgcaccttcc aggcggcgga tgttggaccg gatccggttg gccatgaact gcatgatctc
     3301 ttccggcagt tggacgctca tgagctgcgc cttcttctgc aaaatcgcca ggcgcatctc
     3361 gacatccggc ggctgcaaat ccgtgaccag cccccactcg aagcgcgata ccagccggtg
     3421 ctccagattc tggatttcgc tggccggtcg gtcgcaggtc aacacgatct gcttgtgcgc
     3481 ctcatgcaag gcattgaagg tatgaaagaa ctcctcctga atccgctctt tcccggccag
     3541 aaactgaatg tcgtcaatca gtagcacatc ggtttgccgg tacttcttgc ggaagcgcgc
     3601 cagttggttg ttctgaatgc cctcaatata ctcgttggta aacctctcag acgaaagata
     3661 aacgacccgc gcgcctttct tgtgctgcgt cacgtgctgc ccgatggcat gcaataagtg
     3721 ggtcttgccc aagccgaccc ctccatacag gaacagcggg ttatacgcct tgccgggcgt
     3781 ctgcgccacc gcctgcgccg ctgcgtaggc aaaattgtta ttgctgccaa ccacgaaggt
     3841 gtcgaacgtg ttcttcgggt taaccttcaa ctccaggttg ccctgccctc gatccagcgc
     3901 gagctcggcg actttcttct tcgtggccgc cgtggaagcc gacgacaatc ccggcgcttc
     3961 gctgccgctg gtcccggcca ctttgaagtt aatctggagc tgctgtccgg acgctgccgc
     4021 taccgtgtct tgcagcagat caaggtagtt ctccttgagc caaacctcgc aaaagtcatt
     4081 cgccacctcg agggtgaggg tgccattctc ctgcgaacaa acgcgcagcg gctcaaacca
     4141 gaggttgaac tgatccgcgc tcaacatcgt gcgaagttgc gcgcgtgcca cgttccagat
     4201 tttatctgcg gaagcttgca tttttggccc ccaaacccgc ttttcgggca atttattcac
     4261 ttagccgcca gttcccgctt aaatgccgcc ttcgatagca gtttgcacca tcccgtcagc
     4321 ctcgcctttc aaaaatcgct gtttcataag gaaatctcca ttctgtttaa attcagtgca
     4381 ctctataaat ccatttaaat caataactta caaccaaaac tcacaaatgc tctttattcg
     4441 atgctttcgc cgccccacac gcaaaaacca atttggcgaa ctgaccgatg cttttaggca
     4501 acgctcttaa gccctacgag aacaagatat taactcttat taacgagtta ttcacagcct
     4561 gatttccctc aataaaatca agccatttct cttcttttcg ccgctcgcag cgacccggaa
     4621 aatcaaaccg cgcgctcccc ggtttcctcg gtccgaatgc gaattgcgtt ctcaatattg
     4681 ctgacaaaga ccttcccgtc tccaatcttg ccggtcttgg ccgctttgac gatcgcctcg
     4741 accgccgcgg cagtctggcc atcgggcaaa accacctcaa tctttatctt gggcagaaag
     4801 tccaccgtgt attcgctgcc gcgataaatc tcggtgtggc ccttctgccg tccgaatccc
     4861 ttgacttcca aaaccgtcat tccctcgacg cccagacccg acagagcctc tttcacgctc
     4921 tccagcttga acggcttaat gatggcctcg atctttttca tgtgctgctt cttctctatc
     4981 agggaaagct tagcagcctg tccaaggctg taaacaggaa tgtttccgtg ccgcgggaaa
     5041 tatttttcat cccaaacctg attgtacttg caattgccgc ggccgcgcca atccgccgtt
     5101 gcaagcaacg gcgcaaagga acgccgccaa tcttccaccg acgccgacgg cacgcgcggg
     5161 caaccgggcc atccgttcag tttccgcccc gggtccgccg cggccgttcc gcgcccggcg
     5221 gatcacttcg ccaacatcag gccttcaacc ctttgaagtc ggcctccagt ttctccttcg
     5281 cctgcatcat ttccggccag gtcaccgtgc cgttccgata ggacgccatg cggcccagga
     5341 tggtggtgag gttgctgcgg acgctggggg taaccgtcgg gttggtccaa tcaccccggg
     5401 tgatgcagtc gtgaaaggtc gcaatatttg cgaccacccc gtcgtgatac aggttgccaa
     5461 ccttgccgtc gctatggaac tccttcgctt tgacggtgac attgccaaaa taatgcgtat
     5521 cggcggtgcc ctgagagcca taaacgcggc acaaaatatc gtcataaccg tagcccgact
     5581 ggcgggaatt aaagctcagc accaggtcgt tcgggaaatg gtaaatcacc gcgaagtgat
     5641 cccagcaatt accaacaaac tcgcgcttgc gaccgccggt gccgcaggcc cgcagcggtt
     5701 cggcccccag catccagcag gcgacgtcca acgcatggat gttctgctcg gtgatgacat
     5761 cgccggacaa cacacggtcc acgccccagg cacgcaggcg aacctcgggg ttcttcgggt
     5821 cctggcgcaa gagctggtcc atcgaatccc aggtcggccc gcaatgataa ctggcttcgg
     5881 caaaaaacag tttgccaatc tcccccgcgc gaaccgcctg caccgccgcc tggtaaagcg
     5941 ggttggcgcg cgtctgaaaa tccaccagaa agcagcgctg ctttgcagta gccgcccggc
     6001 cactggcgtc aatgctgagg catccgggca catccaccgc aaccggcttg gccatataaa
     6061 catgcttgcc cgcctcgaca gccgccgccg cctgctccgg gtgaaagtaa ggcgggcttt
     6121 caataactac cgcatcgagt tgctgctcca gcaaccggcg gtagccctgc agcccggtaa
     6181 aacgctgcgc ggcccccacc tggagttgct cgcccaccgc atccacgcgc tcctgaaaat
     6241 aatccgccac ggcaacaagg tgatagccgc catgctgctc aaagagttcc gcgatccatt
     6301 tgccgcgttt gccacagccg atcaggccga ggttaatttt cgcgttcgcc cccgcggcgc
     6361 gagccagtcc cggcttcagg accgtaaagg ccaggacaga agccccggtt ccggccaaaa
     6421 accggcggcg cgtaaaggga gtggcattgt cagttccgtg cggggtttgc ggggtcgtca
     6481 ttggaatcct ttgctgggtt gcgcttattc gaactgggca acattagacc aggcccgcct
     6541 ccgcctgtca aaccttgtcc gggcgcctct tcgtttttgc gctccagcaa ccccaatttc
     6601 cctcgggttt tgcgcgcacc cgcttcgatg tgtggccaag ccacgcccaa ggcttccgcg
     6661 aaaacggcga gtgtgaggag gcgcgagcca gtccgggagg cgggcggcgt aaggcagccg
     6721 ggacggcggc acttctcacc ccgaagtatc ggttttcccg cctggaactc tgcggcggga
     6781 tgaaggaagc cctcccgcag cctacttccc gaacccgttt ccggggtgcg tcctgccggg
     6841 cctggcacct cccgcaccat cagagacgag cctgacgcat tcccaaggct tcgacctctg
     6901 ccgctcgaac ccacactcgg agggctacgg ttgcctcttt gcgcggaacg aggattctgt
     6961 taagctgcag caagaagtta gttagcgaac tatgcccatt tacgaatatg cctgcccgaa
     7021 atgccgggtg atctttaact ttctgtccaa acggattaac cccgaccgtt cgccggtctg
     7081 cccccggtgc ggcaatcgaa acctgtccaa acagatcagc cggtttgctg ccgtgcgcgg
     7141 gttgaaggag cccaccccaa aaaccgacgc gccgacggac gaatcgccaa tccctgatat
     7201 ggacgatccg cgcgtggagc gcgccatgct ggagatggag cgcgatatgg agcacctgga
     7261 cgaaaacaac ccccgccaca tggcccacat gatgcgcaag atgaaggact tcatgccggc
     7321 cggcagtgtg cccaaagaac tcgatgtggc catcaaacgc ctcgaagccg gggaagaccc
     7381 ggagaagatt gaagaggaca tgggcgacgt gctcgacggt ttcctgggcg gccccgatga
     7441 agagggaggg atgggcggcg ggcctggcgg cggctacacg cgcgacagcg gcctttacga
     7501 ttattgagcc gggcctttga taaccgcatt gcataaacgc accgttgacg caccggcccg
     7561 ccgcggggaa agtgccgggg cgccctgggc actggcccca accggcccgc gtttattccg
     7621 cgccattccg ggcggcctcc cacagccgcc tggccccgca tctttggtca tgggcgcggc
     7681 gaaatcccgg cccgggggac atcgttaagg cctgagaaat ccggccgccg catccccgcg
     7741 catctccctc ccccactccc ggctcgctat gttttcccgc cgcctgaaag ctggatattt
     7801 cacgctcgaa ggattgaact cctttgcgac cgtctattac ttctactatt tctatttctt
     7861 catgcagacc gagtttggct ttggcaacaa agccaacctg gcgctggcgg cgttgaacgg
     7921 ggtcgtctat gccgttatgg cctggcaggg cgggaagttc gcccagcggt atggctatta
     7981 cctcgcgctc aagctgggct ttggcctgat gatcggcgcg ctggccatcg gcagccagtt
     8041 ccattccccc ggcgtgcata tcgcggtgat gtgcgtggcg gtctccggca tgtgtttcac
     8101 ctggcccact ctggaagcgc tggtaagcga aggcgaatcc ccggccggcg tgcagcgcat
     8161 ggtgggcatc tacaatctga tctggtccgg cacgggcgcc ctcgcttatt tcatcggcgg
     8221 aacgatgttc gaaaagctcg gcccccgcag catgttctac gtgccggcgc tgatggtgtt
     8281 cggacagctc ctgctgacgc tctggctgga gcggctggcc cgccagacgc cgaccgcgac
     8341 gacgcctccc gcccggccgc cggagccggc cccggaagcc gaagccccgc cgcccgcggt
     8401 ggcccgctcg cgaacatttc tgcggctggc ctggctggcc aaccccttcg cctacatcgc
     8461 catcaacacc ctcatcgcag tgattccgga cgtggcccgg cgcctcgggc tttcgcccat
     8521 gatcgccggt tattgctgtt ccctgtggtg tttttcccgg gtgggaacct ttctgctgct
     8581 gtggcgctgg agcggctggc attaccggtt tcgctggctg ctggccgctt acctggcgct
     8641 gaccggcact tttgccgcca tcgtgatggc ccccggcctg gccctgctga tcctggcgca
     8701 actggccttc ggcgccgccg ccggcctgat ctattactcg tcgctgttct attcgatgga
     8761 cctcggcgac accaagggcg agcatggcgg catccatgaa gcggccatcg gcctgggcaa
     8821 ctttgtcggc ccggcggtcg gcgccctctc ccttcattac ttttctcact atgcccaaag
     8881 cggcacgctg gccgtcagcg gcctgttatt cctggggctg ggcggcctgc tggtcatttg
     8941 gcggaacggg cgcgccggcg gcaccaatta acttgcccgc cggggcggtt tggcctactg
     9001 gtgagcgtgc cgcgtcaagc gcgggccgcc gttcaaccgg cttaaacaaa cgtatgtctt
     9061 tgctgaaagg aaaattagga atcgtcttcg gcgtggccaa ccggcgctcg atcgcctggg
     9121 ccatcgcgca ggtctggcac ggcgccggcg cgaaactggc tttcacctac cagggcgagc
     9181 gcctcaagga taacgtcgaa gaactcgcag gcaccttcgg cggcgatacc ctcattctcc
     9241 cctgcgatgt gacgcgcgac gaggaaattg cccgggtctt ccaacaggtg ggggaccgct
     9301 tcggccagtt gcacctgctg ctgcactccg tcgcgtttgc ccccaaggac gcgctcgagg
     9361 gccagttcct caacaccagc cgcgcggcct ttcgcatcgc gctcgatgtt agcgcctact
     9421 cgctggtggc gctggcgcgg gctgcggcgc cgctcatgac cgccggcggc agcatcatcg
     9481 gcatgagcta ctacggcgcc gagaaagtcg tgccgcacta caatgtcatg ggggtcgcca
     9541 aggccgcgct cgaagccagc acgcgctacc tggcctacga cctggggccg cggcgaatcc
     9601 gcgtcaactg catcagcgcc ggcccggtca acaccctcgc cgcccgcggc atcggcgggt
     9661 ttagcgaaat gctccggcac tacgaagccc attccccgct gcaacgcaat gtcctgcccg
     9721 aggaactggg cgcagccggc ctgtttctcg ccagcgacgg ggccgccgcc attacggggc
     9781 aggtcctgta cgtggactgc ggctaccaaa tcatgggaat gtaaaccccg gccccgccgg
     9841 gccggacgca catcgccccc gcgctgaacc cgtcaccgcc gccccgggcc ggcaagcgaa
     9901 ccggcaaaga cggaaacccc ataccccccg gcgcaacaac tccgcagctc agcgccgcgc
     9961 cggagaggcg ccgtcctccc ggggccgttg caattggcgc aaaccgcggc gcagggtttc
    10021 aatttcctgc tcgtcggcaa tcttctgccg ctcggccaat tcgagggtct ccagcacttc
    10081 gtccatatcc tcgagcgtgg cgcggagcgt ggcaataatg cgggtgacct cttcaatggc
    10141 ctcctgaacc gccatcggcc gggccggggt gcgcgggctg ggcggcgggg gagccgccac
    10201 acgggccgga ggcgggcgtt tcggaccggg ttccggcttt gcgctggcag acggctcgcg
    10261 ggaaacgtcc gccttcggag cggttcccag cttagtcacc ggcggagcgg gtggaggcgc
    10321 cgcgggtgcc gctgcctccg gcgaaggcgc cgccgcgggc gccggtgcgg gagcttccgc
    10381 cgaagccgcc gccgagggtt gctcggccgg acgatccggg cgccgcccgg ggcttcgccg
    10441 gccgcggccg ccgcgccgcc cgcgccggtg cggacgggtg tccgccgccg attgcggggt
    10501 ctcgttcgtt gcgtcgggtg tcgattcagg aaccatcgtg gccctaaggt gcccggaatg
    10561 gccggaaatg caatggcaaa cgcatctcgg tttcaggcgc aagaagagta ttgccaccgg
    10621 ggcggtttta aggttccctt ttcagcgtga aactgaactt taagcggctc aatctgcaat
    10681 taactcactt gtgggccatc gcccgcggcg gcggctcgac caccgcccaa accgtcatgg
    10741 tcgaactcac cggcgcggac ggcacggtgg gctgggggga agcggcgccc atcgcccgct
    10801 accgcgaatc gctggacacc gtggaagctt tcctgaagcg ggtggacccg cgggggcttt
    10861 ccttcaagga tgtcgagggg agcatggtgt atctggacac gctctcgccg cacgatatgt
    10921 cggccaagtg cgcgctcaac atcgcgctgc ttgacggggc ggccaagctg gccaacaaac
    10981 ccatttacga ttacctgggg ctgggcttcc gcgagaatca ccacgtcagc tccttcacca
    11041 tcggcattga taagccggac gtcatccgca aaaaagtcca ggaagcggcg ccctacccgg
    11101 tgctgaaaat gaaggtcggc gtggccgacg ataaaataaa cgtcaaagcg ctgcgcgaga
    11161 tcgcccctac caagacggtc cgggtggacg ccaatgaagg ctggaagacc aaggagcacg
    11221 cgctggaaat gatcgaatgg ctggcccagg acgggcatat cgagttcatc gaacagccca
    11281 tgcccagcgc cacgccggtc aaagactggg tctggctcaa agaacgctcg ccgctcccca
    11341 tcatgggcga cgaatcctac cacaccatca aggacctggc gcaggccagg gaatgtttcc
    11401 acgccgtcaa cgtgaagctg gccaaatcgg ccggcatcag cggcggcttc gaggccctct
    11461 ccgccgcgcg caaagccggc ctcaagacca tgctgggctg catgatcgaa agcagcatcc
    11521 tgatcagcgc cgccgcgcac ctggccgagt tgtgcgacta tctcgacctg gacggcaaca
    11581 tcctcatcag caacgacccc tacgtgggcg ccacggcgga aaagggaatc atgtcgttcg
    11641 ccaaggcccc ggcgaaatac ggcttgcggg tgagcgcgcg gtaacgccgc ccgcggcccg
    11701 ccgctaaccg atcttctccc accgcgtcac ctggggcggc gcggccagcc acgggccgat
    11761 gcgggccagc gcggcctgca ggtccggctt ccgcaaatgc gcttccaggt gcgccgggct
    11821 cgcccaggtc tcgtggaaca ggaaaagggc gggattatcc agcccctggt gcaggtcgta
    11881 attcaggcag cccgcatccg cgcggctggg aaccagcagc gccagcagct cctgccgcac
    11941 cgcgctttcc cggcccggct gagcccggat ttgcgcgatc accgtaagcg attggttgtt
    12001 catccaccgc atcatcgccg ctgccgcgcc cggctggcaa tcccgattta acccgccgtt
    12061 ccgcgcccgg cagggtcttt tgttctcggc gcctaatctg ggtgcgggcc gctggcactc
    12121 ccgcccgccc ccgccgccgc tcaaaggcca cccaagtttc tctggcaacc tgcggcaggg
    12181 gattatgaag ggttcgcgcc gcccactctc gggccgaaac aagccaccgt cgggcgaact
    12241 ccggcctgtg cgcaagctgg aaggctatta atcgcgggct tgaccgcagc aggcctgcag
    12301 cgtattaatg ctccgatgaa ggacagcctg ccatccgcgg aggctgggcg tttggataac
    12361 accccaaaac gggatgttat ccagacccgt ataatcactt gttggctcaa gaaaagaacg
    12421 atatggctag acattcagaa cacccattcg agaaggtttt agccgtctcg acggaggaca
    12481 tacgcatggc gctcttgtct atgggtaatg caccatgggc ggagttccta cttaatcccc
    12541 gccgtcttcg gggaagtgat ttcctcatgc gctggtctca gggtatctgg agcgaagaac
    12601 gcctcaccca agcggttaac gcgacgggca gatatttcgc attgccttat ggtccgagcg
    12661 gaaccgcacc agataatgat gtccgtgcgt ttgaactcta tttcgagcgc cttgagaaag
    12721 ctggtcttgg taacatcaag cggcccgacc tgctgatcta tcggacaaga gacaaagcga
    12781 ggatagattc agcggtcaag gtgctgaacg gaatcagtga attgccgttc acgcctgaag
    12841 acgatcctaa tatgcaagtg ctgttggagc atgccatcat tgctgtggag tgcgagaaca
    12901 gcctttggcg tgcaaagcaa atgccggact atacatcgcc gttcactcca cagaagcgtc
    12961 ttggcgggca ctacggattg aagaagaccg ctgtggttcc gacgattatc atcaaagagg
    13021 aagatcggga gccgctaaaa agctggcaga aaaagagaag agttccgatt cacgtttggc
    13081 acgccttttt tgacgaagca tttggcatct cattggtgaa cgcggagaaa ctttttgcgc
    13141 agggaaacat cgaaccgacg gagcaggtgt tccaggcacc cggtggagcc acgacaagga
    13201 agataattta caaaatttac tacagacacg cttatccctt ggcgaccacg attgaagagc
    13261 cccaactggt agctgactcg attactgaca agaacggtca cattcttccc tacgtgcggt
    13321 ttgtgggcgg aaagtcccaa ctgagcgaac aggcattgaa cgttttggac tccaccaagc
    13381 agaacggatg aaacagagac ggcatcaagc gcttccaagg acagatcgcg aacgcgagac
    13441 gctccgaacc aaggggcagt tctggacacc cgactgggtc gcggagttca tggtcgccta
    13501 cgcattgctt gatcaaccaa aacaactgct tgatcctgct ctgggtgaag gtgtcttctt
    13561 ccgtgccgca aaacgctatg ccgaattgca tgggtttgat ttagcccttt tcgggcgaga
    13621 cattgaccct gacattctta cacaggctcg ccaatcaggt ttgaaagaca ccgacctgcg
    13681 gaacgtggaa atacgggact ttgtgcttga tccgccgtcc gaaaagtacc cagccatcgt
    13741 cgccaatccc ccttatatac gccatcatcg cctctcggcg ctacagaagg catcattgcg
    13801 cgactttgcg cgaacgacta ccggtagaaa tattgacgga cgcgctggac tccatgtcta
    13861 ttttctcatt agagcgctta acacactgtt accaggaggg cgcctttgct acatcgcgcc
    13921 tgccgacatt tgcgaaggtg tgtttgctca ggcactatgg caatggatta cctcgcagta
    13981 caagttggat gccgttatcg ctttctccgc tgatgcaaca ccatttcccg gcgtggatac
    14041 caatgcgctg gtgttttgcc tacgtaacga aagtcctgcc aagcacttcg attgggtaaa
    14101 atgtcagcgt cagaattcaa gggagctgat ctctttgcta tcgggagata agcacaaaag
    14161 atacgagatg atagaggttc atcgaaggac agttgcagaa gcagtagcaa ccggcctctc
    14221 tcgaccgcct tcgcaagcca tgcctaaccg ctatatacta ggcgactttg cctcggtcat
    14281 gcgcggcatc gtgaccggtg ataatgattt cttcttcatg acgctagcgc gggctaagga
    14341 acagggcata ccggaatccc tattggtaaa agcagtaggg cgaatacgcg atatagaggg
    14401 agaacagttc actcttgaag acattgatcg tcttgaagcc cgcaacagac caactcgtct
    14461 tttgtgcgtt aatggattgg cgttcgagca gctgccgatc acggttcaag cgtatctgaa
    14521 ggaaggtgag aaccgcggac ttgacaagaa ggcactcatt cggacacgca aaccgtggta
    14581 ccgaatggaa actcgaaaga gccccccaat catgttcgca taccttgggc gaagaaacgt
    14641 acgtttcatc agaaaccatg caggtgtggt gccacttaca tgcctccttt gcgtttatcc
    14701 aaagcgcgag gactcggatt tcattgaacg cctttggaag gtactcgcgc accccaaaac
    14761 cattgcaaac ttgcggaagg tgggaaaatc ctacggtggc gacgcgataa aggtagaacc
    14821 tcggtcattg gagcgactgc cgttgtcaga tcatcttgtg caggcagaag gcattgaaaa
    14881 gtatgtacaa ccaaaacaat ctactttgtt cgattgagat gagccaacca gaaccgttat
    14941 tcgggcactt actttaccgc tcatcgttac ggcgagagtg tgactgcatt ctggcgatgg
    15001 tagtgtccta ggattgcgac gatgagcttg ccctccaagg ccgcttggct ttcctgcagg
    15061 gcgatggcga ccgttctccc tcatctaccg aaacttaggc gataacaaaa tatcctgccg
    15121 cgcccggagc ccccgccgcc gctcacaacc ccagcgcctc gaaggcctgc aaataaatcg
    15181 catgcccgaa atcgttggga tgattgatat tgttgcccag caggtcttcc ggtttcttcc
    15241 gggcggcggc gcgctgccag cagtgataca catcggcaaa ggcgcagcgc tctccccgcg
    15301 ccacgcgctc ggtcgccgcc gcgtacgcgg ccatgttatg cgagccgtag tgccactttg
    15361 gattcggcgg aaacgccgaa aagagaatca cttccgcccc ggtctccgcc cggatgcggc
    15421 ggaccatgct ccgcaagttc gccgcaaacg cgggcagcgg caccccgaac ccctcgcggt
    15481 tatggtcatt catgccaaaa ccgatcagca ccaaatccgg ctgctgccgc agcaccttct
    15541 cctccagccg ctgcaggccc tgcgccgtcg tgtcgccgcc ggtggcgccg ttgcgggttt
    15601 caatccgggc ccgcgggtac ttggcccgca gcgcgtccgc ccagcgctcc caaaaaatca
    15661 accccggctc cgaggcgtcc ccgcccgccg tgatgctgtc gccaaacgcc accatccgca
    15721 ccggctgccc ggcgcgcagc ttccggcgcg tggccggcag ccgcgccgcc ccgaacgccg
    15781 ccgccccgcg cgggcgctcc cgccgccccg catacgcata gtccacatac acaaagaacg
    15841 cgccgttgcc aaaaccgggg aattgctcat gccggaaatc ctcccggcca tacaaaacgt
    15901 tggtcccaaa atccggaatg cgcgactccg gggtccgccg aatccggttg ggcgcctcca
    15961 gccggtaatc gcggcccgcc tcgtaatgaa tcgtccgcgc cagcccgtcg cggtaggtat
    16021 tgcgaaccac aacggcccgg gccggcgacg ggtcggaaat cagcgtcgcc ggcgcctggc
    16081 ccgccaaaac gaccgattcg cccgtggtca cttgaagggc gcgcggcgcg caaccgctta
    16141 aacccgcggc ggcggccagc gccaggattc cccaaagccg gttgatgcaa cgcatcctcc
    16201 agaaatgccg ggccggggcc ggaagggcaa ctgatttgtg gccgccgcca ttggcggctt
    16261 gtttccgcgc ccccgaaatg actaatgtcg gaaatgacca gtgcggagat acgccaatcg
    16321 ttcctggact ttttccagtc ccgggaacat accatcgtgc cctcagcgag cctgctgccc
    16381 gactcgccga acctgctgtt caccaacgcg ggcatgaacc cgtttgtgcc gatcttcctc
    16441 ggccagcgcg ccccggatgt ctcgaaatgg gcgggcgccc gccccggccg cgacacccgc
    16501 gccgccgaca cccagaaatg catccgcgcc ggcggcaaac acaacgacct ggaggacgtc
    16561 ggcctcgata cctaccacca caccttcttc gaaatgctgg gcaactggtc cttcggcgat
    16621 tacttcaaac gggaggccat tgactgggcc tgggaactca tcaccgaagt atggaaattc
    16681 cccccgcccc ggctctacgc caccgtctat gcgcccgacc cggcccgggg cgaccccgcc
    16741 gagttcgacc aggaagcccg cgacgcctgg gcggcccggt tccgcgccgc cggccttgac
    16801 ccccaggtcc acatcgtcaa cggcaaccgg aaggataact tctggatgat gggcgacacc
    16861 ggcccctgcg gcccgtgcag cgagctgcac atggatctca ccccccgggg cgacagccag
    16921 ggccgcctgg tcaaccaggg cgacgcgcgc tgcatcgaaa tctggaatct cgtcttcatc
    16981 cagttcaacg ccaacccgga cggcacgttc tcgccgctgc ccgcgcggca cgtggacacc
    17041 ggcatgggct tcgagcgggt cgccgggatc atccaaaaca cgcgcaactt caccgacttc
    17101 aaccgcatca tctccaacta cgacaccgac ctcttccggc cgctcttcga ccagctcgaa
    17161 cgcctcagcg ggaaaaaata ccactccacc ctgcccgccc ccggcgcccc ggcgctctcg
    17221 gaaacgcagc aggcggacat cgccttccgc gtcattgccg accatgtccg gacgctgagc
    17281 tttgccatcg ccgacggcat cgaacccggc aacaccgacc gcaactacgt cctgcgccgc
    17341 attctgcgcc gcgccgtccg ctatggccgc gcgctcggct tccgccagcc gttcttcgcc
    17401 ccgctggtcg cggcgctggc ggaaagcatg ggctccgcct tccccgagct ccgcgcccgc
    17461 caggaacgca tcacgcaggt catccggcgc gaggaggaaa ccttcaacaa gaccctggac
    17521 cgcggcctgg cgctcttcga ggaggaagtg gcggcgcggg ccgccgacgc gtccgcggcg
    17581 cgcgcgcccc tctccggcgc cttcgccttt cggctgtatg acgaacaggg tttcccgctg
    17641 gatttgacgg aactgatggc gcgcgagcgc ggcctgacgg tggacgtcgc cggcttccac
    17701 cagctcatgg aggcgcaaaa ggcgcgcgcc cgcgccgccc agaaaaagca ggtcatcgaa
    17761 ctctcccgga tcacgaccac aacccccacc cggttcgtcg gctacgacca gtgggaaacc
    17821 gccgccaccg tgcgggaagt ggtcggcgtg aaggataaaa ccgccgtcat cctggactgc
    17881 accccgctct acgccgagat gggcgggcag gtgggcgaca ccggccaact ggaacgcggc
    17941 ggcgaaacct ggcgcgtcgc ggacacgcag aaagcgggcg acgcctggct gcactacctc
    18001 gcgggccgcg acgcgccccc cgccggcgcc gcggtgcggg tggcggttga ccgcgaacgc
    18061 cgcgccgcca tccagcggca tcacaccgcc acgcatttgc tgcattgggc cttgcacgag
    18121 gtggtcagcc ccgacgccgt ccaaaaaggc tccttcgtcg gcccggaaaa gctcaccttc
    18181 gacttcaacg gcgccccgct caccccgcgg caagtggccg acctcgaaca ggcggtcaac
    18241 gagcgcatcc tcgaaaacgc gcccgtcacg tggaccgaag cccgctacca ggatttgcgg
    18301 aaccgccccg acatccggca gtgctttggc gagaaatacg gcgacgtcgt gcgcgtgctc
    18361 caggtcggcg gccagcccgg cgcgctgaac ggctactcca tggaactgtg cggcggcacc
    18421 cacgtgcggg caacgggcga aatcggcctg ttccgcatca cggccgaatc cgccatcgcc
    18481 gccggcatcc ggcgcatcga agccctcgcc ggcctggagg cgtgccggcg aacctccgag
    18541 gaacggcagc tcctccgggc cattgcggac aaggtcaact cgcccctcgg cgaactggaa
    18601 aagaaaatcg aagcgctgct ggcccagcag cgagccctgg aaaagcaact gctcgcgctc
    18661 acccaggaac gcgctgcgca gaccgcccgc aacctcctga gccaggcgca gacgctcaac
    18721 ggcaccccgg cgctcatcgc gcgcttcgac gccgacggcg agcagctcca ggggattgcc
    18781 aacgaactga aaggccagtt ccagggcgtc atcatcctgg gcggcgtggc ccagggcgcc
    18841 gtctccctcg tagccgcggt ggcgcccgcc tacgccgcca aatacccggc cggcaaaatc
    18901 atccagaccc tcgcgcccct ggtgggcggc aaaggcggcg gacgcccgga caacgcgcgc
    18961 ggcggcggca aggacacaag caagctcgac gaagccctgg cccgggctcg aagcctgctg
    19021 agcggggctt gagcacgcgc gggcattcct gcccgttccc acaattctac gcctgacgac
    19081 gggcaaaaat acccgtgttt ccgccgcctg cttccgccca aatccagtcg cactcaggca
    19141 ccgttaacga taccgaaccg tgccaaagtc gaaaaatcgg cctaaacagc caatttcgcc
    19201 ccccaattcc cctcatattc gaacgccccc tggaggcgca ccgcctgccg ggaaaacatc
    19261 ctccaaatcc tgaattcatg acggaatccc caaaaaccgg cctttccgga catccacagc
    19321 cattatccag ataccgtgaa aataccctat ccctacgccg tgtacaaggg gtgtacaagg
    19381 gatgcccaac ggatgtacca gctaaaatct catggattcc cggtgagcat cccttgtaca
    19441 ccccttgtac atggcctgca gtcagggtaa atgcagggta aatgcaaggc aggttcaagg
    19501 acggtggtta tggggagaaa ttgggccgga aaagcgctgg aaccgttgtt taaggcggaa
    19561 aatcggcatg gaaatccctg ccaaacgcac taagcactat aaaatcaata gttaagaacg
    19621 cgctgccgca taattgggga gtttgagacg ccgccggcgt ttgccgcgtc gaaaacggct
    19681 gctgcttatc cccggctctg ggggttcgct ccccggcggg attccgggtc acggaaaacg
    19741 ctcgcccggc gggcggatag gcttcatgct ggaggcatgc atctggccca tttgcgcctt
    19801 cgagacttcc gcaattacgc gcggttggat gtggattttg cccccggcct gcacctgttg
    19861 ctgggcgata acgcgcaggg caagacgaat atcctggagg ccatttatct catggcgacg
    19921 ctgcgctcgt ttcgcggcgt gggcggggcg cagatgatcc ggcacgggca gagaggctat
    19981 ttcgtcgggg gcaaggtgat cgggcagggc gagcataaca tcaaaatgta ttggtccgcc
    20041 gcggagcgga acctgagcct gaatggccgc ccggtgcgca agctgacgga ttacctgggc
    20101 gcgctgcgca cggtgatttt ctgcacggaa gacctgcaat tagtgaaggg ggcggggcgg
    20161 gcgcggcggc ggtttctgga cctgctgctt tcccagacgt atccgggcta tctgccgctg
    20221 ttgcagcgtt atgcgcgggc gctgcgcgcg cgcaacgcgt tgctgaaatc cgccggggcc
    20281 gacccgatgg cgctggatgg gttttccagc gagctgatca aggcgggcga cgaggtgatg
    20341 cggcggcggc gggagctggt tccgaagctt tcgccgctgg cccggctggc gtatcggcgt
    20401 atttccaacg acgccgagga gttatggctg gattatcggc ccagtgtgaa gcgcgacttt
    20461 gccctcgaac tggcgcaatc ccgctcgcgg gaacgggtgt accgcgcgac gctggtcggg
    20521 ccgcatcgcg acgaactgga gctgcggctg aacgaccgcc cggcggcggc gttcggcagc
    20581 gagggccaga agcgcacgct ggccatcgcg ctcaagctgg cgcaggccga atacctgacc
    20641 ggcttgcacg gggcgccgcc gatcctgctc attgacgaca tcatgggcga gctggatgcg
    20701 aagcgccgcg gagggttcct gcctttgctg gatcgagccg agcagtcgcg cggccaggtc
    20761 tttatgacgg cgaccgaagc caactggccc gtggaaaccg ggcgcacggc gcggcgctgg
    20821 ctggtgaaag aaggcacgct gttgtccctg cccgcttaac cggccgcgcg gttaggcccg
    20881 gcctgttcgc cccgccccgc ggctcgcccc ggtccgcgcc cggcgccggc caaagccggg
    20941 ggaaaccgtt aattgacgaa ccccttcggt gctttagaat gacccgctat gcgtgatagt
    21001 cgttggaacc ggaaagtgaa cgcgcccggc gggcggcccg gactgaaggg cggcggcggg
    21061 gtgctggtcc tgtgcctgct ttggctggcg gccttgccgc tgctggcgga gagctattat
    21121 ctcccggacg gacggccgga cggggtggcc ctgttggcgc cgccgccgct tcccggctcg
    21181 gcggaggaaa cggccgacct gcagacggtg cgcacggtct tccagggacg gacggaagcg
    21241 gagaaggagc atgcgtttaa gagcgcctcg ctttcgatct tcctgtatgc gccggcgatt
    21301 gggccgttct tccagccggg caagtttccc aaggtcgagg cgctgtttca aaaggtgcgg
    21361 aaggatatct ccgcgccgct ggaccgcacc aagaagcact ggaaacggcg gcggccgtat
    21421 gaattggatc cgcaactggc cctgggacgg cccgagacca cctttagcta cccgagcgga
    21481 cattccacgc gcggcaccgt tcaggcgctg ctgctggccg agttgtttcc gaagcagcgg
    21541 gaggcgatcc tggccattgg ccggcagata gggtgggacc gggtgctcat cggcaagcat
    21601 ttcccgaccg acatccacgc cgcgcgcacg ctgggacagg ccatcgcgcg cgagtttctg
    21661 gccagcccgg ccttccagcg cgacctggcc gaagcccagg ccgaagtgcg ggcctgcccc
    21721 gaggccaatc tttaacgtga atgcgggctt gcgtttcaaa gccgctccag caacatttat
    21781 ggtgcccgcg tggattcgcg gacaataata acttagattc gatcaatatg gcatctgcga
    21841 acgaccttcg ccgcggcatg gcgattaact ataacggtga tatttgcgtg gtgttggaca
    21901 cccagcaccg gacgccgggg aacctgcgcg cctttgtgca ggccagcctc cgcagcattc
    21961 gcagcggacg ctccgccgat gtgcgcttca gctcgaccga gaagatcgag gtcgtgccga
    22021 tgatgacccg caagatggat ttcagctaca aggacggcca ggattacgtc ttctccgatc
    22081 ccgaaaccta cgaaacggtc atgctcacgc cggaattgat tggcgaggcc cggaatttcc
    22141 tggtcgaaaa cggccaggtt accgtcacgt tcgtcgaggg caaggcggtg gcgattgagc
    22201 tgccctccag cgtggtgctg acggtggcgg atgcgcccga gggcatccgg ggagattccg
    22261 ccaataacgt ccaaaagtct gtcaccctgg aaaccggcat caccatccag gcccccctct
    22321 tcatcaagac cggcgagaag attaaagtag atacccgcac cggcaaatac atggagcggg
    22381 cgtaggcggc cccgctgcgg cccggttcca ccgccggctt ccccggcccg cccgcgcggc
    22441 gggtcgtggc gttttccggg agaatccgcg ccttaatccg ccccgtccag cggcgcgccg
    22501 aagccgaact tgcgggccag ggccagcgaa gtattcaagg ctttcacccc cgtcgtgcag
    22561 gtggcgtccg ccagcgaggc cgccgcgatg caaacccccg tcagcaggca ccgctgcaag
    22621 tcccaacttt cgtgcaagcc atacagcacc ccggcgcaga acgcatcccc ggcgcccgcg
    22681 gtgccggcga tgtatttggg cggcaatttg agggcgggct gccagaaatc atcccccttg
    22741 cgcgtgcgcg cgaaggctcc ttccgggaaa tgcaccacca ccagctcgcg cacgccctgt
    22801 tgcagcaggg cgccggccgc gtggcgcagc gccaccgtgt ccagtttccc gtccggcagc
    22861 cgaatcttga acccggtcgt cttgcccgcc tcgatttcgt tgaggatgca gtagtccaca
    22921 tgctttaacg ccgggttgac aatgcgcgcg aagcggtcgc tgtcttcgct caccacatcc
    22981 acgctggtcc gcacgccggc ggcctgggcg cgggccagca ggcgcgccgc ctgggtgccg
    23041 agccgggcgt cgggcttgtc cagcgcatcg agcagcagca ggtatcccag gtgaaagatc
    23101 cgcacgttga gcttctggaa atccaaatcg gcgccgtccc aaagcgcgtt ggcgccgcgg
    23161 gcatgaaaga acgtccgccg cccgctgccc agctcggtca tcacgtccgt gtaggaggtg
    23221 ggggccttgg gggtggcgcc gaggtgccgg acatcaatct tgtagtggcg gcagatttcc
    23281 aggatttctt tgcccagggg gtcccgcccc accaggccgg cggcataaag tggaaacggc
    23341 gtgcccgacc gcgcgaggtc caccagcacg ttaaagggcg ctccgcccgt gccctgggat
    23401 tggctgcgaa tgttcgccaa ctgctcgggc tgggggtaaa cgtcaatcat cttgacctga
    23461 tccatgatcc agttgccccc ggcaagcaga ccggagcgaa tcttggcgct ttttttcttc
    23521 ataagctaaa ggcgtattct aggtcttagt gaacgaaaaa ccgccgcccg ctggcaagcc
    23581 ctttcttccg gccaccgccc gctttgccgg gccggaccga aacggcgggg gaaacccggc
    23641 aaatccggcc tctcccaacg ccccgagacc cgggccagcc gcgcggcggg gcaaggggcc
    23701 gagcttgacg attccggctt gattgggcag actataacca tgatgcgcag ggccgacaac
    23761 ccgctctgcc tccgccccgc ggcatggcga aagcggggga tgtgcccaga gactttatat
    23821 tcgccgaaca acccatgacc aaaggatctt catgcaactg acgagattga cgagcggcgg
    23881 gggcgggctg gcgctggggc tgagcctgct gctgggggcg ctcccggccg gcgcggcgcc
    23941 gccgcggctg acgccgctgt cgcaaggcca atggcccgct tacgcgcggg gcgcggcgga
    24001 ggatgtcaaa atggcgggca gctacgcttg cctggccctg ggaaccagcg ggctggcggt
    24061 gattgacgtg agcaacccgg cccacccggt gcggacgggc ggctgcgata ccgacgggca
    24121 ggcgggcggc ctggcggtgg cgggcaacta tgtttacgtg gcggatggag aggcggggct
    24181 ggagattatc agcatcagca acccggccca cccggtgcgg ctgggcgggt ttaataccag
    24241 cggccaggca aaggacgtgg tggtggcggg caacctcgcc tacgtggccg acggcacggc
    24301 ggggctgcag gtgattgatg tgagcaatcc ggccagcccg gtatggctgg gcggctatga
    24361 taccagcgga tatgccaatg gcgtggcgct ggcgggcaac tacgtttacg tggccgacgg
    24421 gccggcggga ttgcaggtca tcaacgtgag cgacccggcc cacccggtgc ggacgggcgg
    24481 ctatgacacc agcgggtatg cgaacggcgt ggcggtggcg ggcggcatcg cctacgtggc
    24541 cgacggagag gcggggctgg tgattattag cgtcagcaac ccggccagcc cgtctttgct
    24601 gggcagctac ctcacctggg ggaacgcgaa ccgggtggcc gtggcaggcg gcgccgccta
    24661 cgtggccgat attctgacgt cgctgcaaat catcaatgtc agcaacccgg ccaaccccat
    24721 gccgctgggt ttctatgaca ccggcggcgg cgcggcgggc gtggcgctgg cgggcagcac
    24781 cgcctgcgtg gtggatgccg ccgccggcct gcaagtcctc aacgtgagca atccggccga
    24841 cccggtgcgg ctgggcggct ttaatcccgg cgggacggcg tatggcgtgg cggtggcggg
    24901 ctcgctggcc tatgtggcgg acggcgcggc cgggctgcaa atcattgacg taagcgatcc
    24961 gctcgccctg gcgcggcggg gcggttttca aaccaacggc ctggcgcaaa gcgtggcggt
    25021 agcgggcacg accgcctacc tggcctgctc cgactccggg ctgcaaatta tcaacgtgag
    25081 caaccccgac agcccggcgc ggctgggggg ctacctttgc tcctacgcca acggcgtggc
    25141 ggtggcgggc tcggtcgctt atgtggcgga tggcccggcg ggactggaaa tcatcaacgt
    25201 aagcaacccc gccagcccgg tgcggctggg cggctattat tccgccaact gggtcgcgca
    25261 gggcgtggcg ctggcgggca cgaccgcctg cgtggcgtat ggcgaagccg ggctgcagct
    25321 tatcaacgtg agcaaccccg ccaacccggt gcggctgggc ggctacaaca ccagcggaac
    25381 ggcgtatggc gtggcggtgg caggcgcggt ggcctacgtc gcggacggcg aagccgggct
    25441 gcaaatcatt aacgtgagca accccgacag cccggtgcgg ctgggcggct gcggcaccgg
    25501 cgggatagcg cggggagtgg cggtggcggg cgcgcgggct tatgtcgcgg acgacgaagc
    25561 ggggctgcag gtgattgatg taagcaatcc cgccagcccg gtgcggctgg gcagctatcc
    25621 cacgcgcgga acggcgcggg gagtggcggt ggccggaaac cgggtttacg tggcgaacga
    25681 cgaaaagggg ctgcgggtct tttgctcgct gccgaacgtg cagtacatgc tgcgcgtgga
    25741 ggggggcgcg ctcggcacgc cgttcaccct tgaggcggcg tcccttctca aggcggcgac
    25801 caactggcag gcgctcttca ccaccaaccc ggcggcggtg cccttcgagt tcacggatac
    25861 cgacgtgcgc tacgccacgc agccgcggaa gttttatcgc gtgcgccagc cgtaacggct
    25921 cgcggcagcc ggccgggaac cgccggaggg ccggtcaggc ggcgcccaac tggaggcggg
    25981 cgtctgcgtt cagcagggtg gcgaggttgg gccattcctg ctcattcatt tcgttgaagg
    26041 agtggagata gaccgcgacg gtctcgggcg ggtatttggc caggagcgtt tccacggcgg
    26101 ccttaagttt ggcgtcgtcc atcgccggcg gcaagtcgtc caccacgccg tcctcgtgct
    26161 tgatgcccag cgcgtccaga aagtcggtga gcatggcctt gtgctttttg aggagccagg
    26221 cgcggatgag gccgctggtc accgcgtcga gggccgggcg ggccaaggaa acgagcatgg
    26281 ccgcatggcg ctgcggcttg ggctggcgct cgaaatacac cgggcgcagc ttgcgcgcct
    26341 ccgcgaccgc attcagggtg gcgcggtaga tgggtttatc gttctcgtag gcgtaggtga
    26401 ggatttccat cgccagggac ggcgacatga aaccaagcag ttcgtgggcg gagagcatgg
    26461 atgcggggcg gtttggatcg agcgtgttaa cggatgacag cgtgactcgg gtttactggt
    26521 tcagttctga catggtggac gaggggcagc gccaatcgtc gcgaccgaaa tcttatgcgc
    26581 gcgggccagg cgttcgcaag tctcttgttc caggagcagc gagcgccccg cctcgaaggc
    26641 cagcaccgcc acccgcgcgg cggcgcaggt ctcgagggtc tgcgggccga ggcaggggat
    26701 atcaaaccgg aaatcatgcc gctccttggc caccttgacg gcgaccgccc cgccgtcggg
    26761 cccggccagc gcgccgccgc gggccaggca ggcgtccgtc ccctcgaagg cctcgaccgc
    26821 cagcacggcg ccccctttga cgagaacaat ctggcctatc tccaggcggg atacttcctt
    26881 ggcgatgcga aagccgtatt ccacatccgc gcgctgagcg tcggacagcc ggggcccgag
    26941 gtggaacccc tcgcccggca tcaacggctg aagccacggc gtggcttcga tcagctcgac
    27001 gccgtcgcgc tggagctcct ccgcgatggc gccaaagatg gtgtgggcat ttttttcccg
    27061 caggcgcaac agcaggcgca tcgcacggag gtcggggcgg agatcgaaaa ggttccgggg
    27121 cgcaacctgg cccgccatca cacagtgcct gacaccgcgc tgcgtgaagg cggcgatcat
    27181 ccgggaaagc tggccgacct tgagccagac gatctcatcc accagcgctg ccagcgccgg
    27241 atcggtctcg ccttcgaccg caacggcgac caggcgccgc actccctggc ggcgggcttc
    27301 ccgggcgagc aggagcggca gggaacgatt gccggcgatg atgcccagcg tatccggaag
    27361 gcgggtcatc gggattcccg gggattgggg agtgacgccg gcgcgaaaag cgcgcgcgcc
    27421 ccggcagccg cctcccgctc cgggaggccg cagggccaat cggccgggct ggcggtgacc
    27481 agaaacccgg gctccattct cgcgcgcatt tcccccagta aagcgcaaac ccggcggtct
    27541 gtaaatccag atgacgcgcg gcttacggct tcgcgggctt gctctccgcc ggcggggatg
    27601 cgggcgccgg gggcggcggg ggagacaggc gattgatttc cgcctggtaa ttctcggcct
    27661 ccgcctgctt gcccagcttc agggccaggg gccgcagctt ctggtaaagg gcgagcggtt
    27721 ccgggtaatt cggaaagtct tgcagcagtt gctggtaatc ggcgtaggct tcctccgtgc
    27781 gccccagggc ggacagccgt tcgccgatcg tgagcaggag ccggattttc tgctgaggcg
    27841 aggaggccag cggcagcgcc tggcggtagg catggacggc cgaggaaggt ttgccctgcg
    27901 ccaggtataa gtcgcccagc ttctccgtca gcacggcgct ctggcgcgtc gcctccagtt
    27961 gctccagcag cgcgacgacc tcccgcaccg gcttgccgac ggccaggttg agattggcca
    28021 ggcgcagcca cgaccattcg atcagcttgc tgccgcgcgc ctccaaatcc cggtgccggc
    28081 ggtcgggatc catgccaaac ggccggtaaa gcgggtcgcc caccacggtg gtttgccacg
    28141 acaggaccga ttgcgccgcg taggcggctt cgccgaaggt gaacccgaga taaaccaggc
    28201 gcgcggtgaa cgccgccagg tcgggcgtgc ccgaaaggta aggctcgcag acggttccca
    28261 tggtgatggt gacgcccttg gccagcagcg ggccgaccca gtgccggtgg gcggcgcgca
    28321 aggtccccgc gctgttggaa tgcaggtggt aagcgaacgc gccgggcatg aactcgacct
    28381 ccggctgcgc gaaaggcccc gagacatgct catcgtacca gccgagataa taggcgacct
    28441 ggctcagggg aaaacccgcg gcaaaggtgc gggcattttc atccaccacg gtttccaagc
    28501 ccaactgctt gcagactccc gcgctggcgc gaatccactc gtcaccaacc cggtagccgg
    28561 ggtcgctgat gttgcgcagg tcaaaatagg cgcgtcccca gaggccttcg gcctcggcct
    28621 gcagcgcttt atccaccagg ccgcgcgcga tggcagccgt ggggccatcc agccgtgcga
    28681 ccatcagcac gccgttggtg gggctcagcg tcgcggcgtt ggtggtggta tagaccgggt
    28741 tgcgcagcgg gccaaacagc ggcagcttct cgaccgtcat cggcagcagg gccagttcat
    28801 tatccacggc ggcttcgtcg cgccgcaatt ccggccgcag ccccgccatg ccttcctcct
    28861 tcaagtgcgg gtcccgctgg atgcgcagcg ggacgccata gcagagcacg gcgtagcgga
    28921 tggcggaggt gaggggcttc cactccaccc ggcccggctg gttggtcgtg gcgctgataa
    28981 tgcacgggcc aatctcccac cgcttctgct ctttgagctt ccgcgccagc ggctcggcca
    29041 gcgcgctgcg atactccacc cgggacatct cttcgttcgt ggacaggggg aacccgtaaa
    29101 tctggctggc cggcacctgc cgccgctccg cgtagtagct ggccacctcc cgggattccg
    29161 gcacgcgcgt attatacacg atgataacct cgtcccccgg gccggccgcg cgcacggctc
    29221 ccacgccccc gcccgccagc agcatcagcc ccgccaataa tctcttcatc atcgctatga
    29281 gtatggacat ccgcgcgccg gattagttca agcgccgccg gccacacgca ggccatcata
    29341 cgccagctcg actcccgggg gaagctccgc ttgcgccgcg tcgtggtcgt agtcatgcgt
    29401 caaatgcgta aagtaagtcc gccccgcgcc gatgcgccgg gccgcggcca gggcctcgtc
    29461 caggcacata tgcgtcaggt gcggccgggg ccggagcgcg tccagcacca ccgcttccgc
    29521 cccgcgcacc tgcgcgacga cttccgccgg cacctccttg caatcgctca tataggccag
    29581 ccgcttccgc ccccgctgaa cgaaaagata gccataggtc gtggttcggc catgcggcag
    29641 ggacagcggc gtcacctcga gatccccgag cgtgaagggg ccggtgacga cgtggggttc
    29701 gggagtgaag tagccccggg gccgcggccc attctcgaag gcgtagcgga agacgcgttt
    29761 taaatcgttc atcgtggctt cgctcgcata caccggcagc gcctggccgc cgcgcaggtc
    29821 gcaaaagcgc cggcaatcat ccagccccat gatatgatcg gcgtgcgagt gcgtaatgag
    29881 caccgcatcc agccaggcga tcttctcgcg cagcatctgg aggcggaatt ccggggtggt
    29941 atctatcacc agccgggtct gttccgtcgc cacatacaac gaaggccggg tccggtgatt
    30001 cctgggattg gcgagaaacg cgggtggata ctccttgcca ataacgggca cgccctgcga
    30061 agtgcccgaa cccagaaaga cgaaagtaaa tgacattgtt cccctcacct tagcc
//
LOCUS       DAOPQG010000036        29964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_1987_length_29964_cov_14.966231, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000036 DAOPQG010000000
VERSION     DAOPQG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 29964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29964
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1987_length_29964_cov_14.966231"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..813
                     /locus_tag="PKI07_05860"
     CDS             145..813
                     /locus_tag="PKI07_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOC50613.1"
                     /translation="MTEYAAQCKKVLGRFVNFVQAQNPKAVEIALVTRKLAQAFMGAE
                     AERGVGAKTWNDTLKLLRATFKYLLPAGAGNPFYNMVTKDAETVFRKPFTPEELKAIL
                     DAAQDDDFIRPIIITGMCTAMRRGDCCRLKWKDVDLARRFITVKTAKTGQTVSIPIFP
                     LLLGELASLPTQGGELVFPEQAEMYKTDRKLLFTRPHVLSAAWVIVSVTGRARSPTLR
                     ARCS"
     gene            complement(740..1171)
                     /locus_tag="PKI07_05865"
     CDS             complement(740..1171)
                     /locus_tag="PKI07_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50614.1"
                     /translation="MISFMPQIHQRVYERNCLNYVYNKIREPLWNVLVEYVGLPVASP
                     GGVLRDEILVRLPAPEYGIRLGQSRNRSVLTLELRQRVTGCGGRMFHDRQTIPIGDMR
                     DGVLLNVDHLATTKTLRTNYTVPEVLGSERGLSRKLSPTQR"
     gene            1347..1517
                     /locus_tag="PKI07_05870"
     CDS             1347..1517
                     /locus_tag="PKI07_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50615.1"
                     /translation="MRARNEGAGVLLVSSELDEVLTLSDRILVMFEGRMVGKFRRGEV
                     TERELGLKMGGA"
     gene            2145..3758
                     /gene="infC"
                     /locus_tag="PKI07_05875"
     CDS             2145..3758
                     /gene="infC"
                     /locus_tag="PKI07_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOC50616.1"
                     /translation="MKKFRVNQQIRVPEVFLIDENGQSIGVMPTAKALSLALEAGLDA
                     VEVNPKVQPPIVKIIDYGQCRYKKEKEAQKQKVTRAALSTGGRKKQISSGRSESKTAV
                     QKALSCPFRCRLKLSRALMIAAAFSPVTSLAGWRRILSRRWLKKQWRILQCRIRRRLF
                     CSATVIAVTGSCGKTSTTHFLGKILSAEHSCRIGISDNGQEAMLENLRRLNRSHRFVL
                     QETGVNGPGSLNEIVPILRPHIGIVTSIGQDHYRNFRTLEATAAEKGILIESLPDSGV
                     AVLNADDPYVIAMASRTKARILTYGLSDRADVRATDIRSTWPERLSLTVTYQGTSIRI
                     ETGLFGTLLSNSVLAAVSGALAAGIGLKQCAAALKSVETFSRRMAIHSTSRRVWYILD
                     TVKAPYWSIGKVLALMADVDAPRKTVVIGSFSDAPGSDSDKYRCLARDALNIADRVLF
                     VGRKAIHINKMLTAETHGRLFAMESLQEAAKLLADDVVADEVVLIKSGNLEHAERLYF
                     SQDNILRCWKPSCRFRSSCEYCTESGLSG"
     gene            complement(4046..4261)
                     /locus_tag="PKI07_05880"
     CDS             complement(4046..4261)
                     /locus_tag="PKI07_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50617.1"
                     /translation="MNPLRCPVCQNPMRVIAVIDDRRVAEKILRHLGAWHDPPPRPPP
                     QRVPGPYTYEPCDDVDPMPDYENVLTD"
     gene            complement(4763..5812)
                     /gene="lpxD"
                     /locus_tag="PKI07_05885"
     CDS             complement(4763..5812)
                     /gene="lpxD"
                     /locus_tag="PKI07_05885"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOC50618.1"
                     /translation="MPYTVADIARLTGGEITGDASLVLNGFAPADRSQPGDLTFAENA
                     NYFARAEQSAASAILVDSAYTSRGKTLIRVPNARVAFAKVLPLFFPETPFAPGIHPTA
                     LVPASAQVDPTAHIGPYCVLGEQVRIGARSVLQGLNHIGANCVLGEEVNLFPNVTLYP
                     RTEVGHRVRIHAGTVVGADGFGYVLDNGVHRKVPQIGNVIICDDVELGANVTVDRGAL
                     GPTFIGRGTKVDNLVQIAHNVTLGEHCIIVSQVGISGSTRLGNYVVLGGQVGLAGHLR
                     IGNRVAVAAQSGVMHNIPDGEKWLWTPAQPDRQAKRQMIALQQLPELLRRVKELEKRL
                     EAMGEPPAADPPPTS"
     gene            5996..7186
                     /locus_tag="PKI07_05890"
     CDS             5996..7186
                     /locus_tag="PKI07_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50619.1"
                     /translation="MSNRPLNVGLVGGGKGAFIVHPHQRAIHFDGTRRVVAGALFPDP
                     RIARAEAAQWPYPLKGYGSYDEMIAANAQLPEAEKLDYILIVTPNFVHFDPAMKAMKA
                     GIAVFCEKPLCLNLKEAAALVKTARQLNIPFCVAHTYVGHWTSRLSRYIVRSGLLGEV
                     RWVDSSYLQGWLATRLEATGQQQASWRVDPKRAGGSGCGGDIGTHALMQLRYVTGLEV
                     TALSAQLETFVQGRQLDDHFTIYCQLSNGGKALVRASQICIGYKNDLGIVVAGTKGTL
                     CWRQEEPESVTIKLPGQPDRVYWRGAVTPGDGFLPKNVPADLMAEPTIPSGHPEAFHD
                     AFARLHRCFEADVRKWKAGKAFKCDGAKYANVEDGWTGIAFIETCVKSSKKKGAWTPM
                     PKAI"
     gene            7586..7804
                     /locus_tag="PKI07_05895"
     CDS             7586..7804
                     /locus_tag="PKI07_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50620.1"
                     /translation="MTITNTQLELSLGNRQTHRLPSATSNSSGHRAQWWFRRMRQIVD
                     CALDWQPAPPPRPQQTSFASMDFSAWTA"
     gene            8319..9593
                     /locus_tag="PKI07_05900"
     CDS             8319..9593
                     /locus_tag="PKI07_05900"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC50621.1"
                     /translation="MSNQLAINGGPKAVTNKLQSWPQFDENAIKAVETVLRSGKVNYW
                     TGKQGMEFEKQFAAWQGSKYAVSVATGTAALHVALNSLGIGPGDEVIVPSYTFIASSF
                     SIVQAGAIPRFADVNLEDHCLSVASAEKLVTKRTRAIMPVHLYGNVCDMDQIQAFARR
                     HNLYVIEDNAEAFGGVYKGRKTGTLSHLAGCSFCQNKTFTTGGEGGMVTTDDEDLAWK
                     ARSFRDHGYDVKQRLSLLELEQKLPYIHNMVGWNYRMTEMQSAIGLAELARMDTWNLP
                     RRRRNAQILINALKTSEYIKYFPIDTPERQNGWYVLAFSLEVERMKCDIKQFVAAVAA
                     EGAPVWKVFWPQCHTERAFQEHHAFGRTGFPFKSREYADPASVDYREVEVPNARWHEA
                     HTFTCFAYPTFTESDMEQIAEAILKVIKAYAA"
     gene            complement(9574..11091)
                     /locus_tag="PKI07_05905"
     CDS             complement(9574..11091)
                     /locus_tag="PKI07_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOC50622.1"
                     /translation="MKYCVSLLSIGLCVPLLLAAPPAPAAAPVSAPGKPLIIPQPKSL
                     RLGAGTFRLEPGMKWTLRAAARDPRLWQAAEAAFPGAPGAFTRSRSPGVVLQIGSPRQ
                     TVFPAGQARWAQDPEGYRLVVEPGGLAILASTAQGAFYGLQTLAQLWAPAGRARTCPA
                     LEVDDWPALRFRGAHWFPSASGAPMHQRLITNVFSALKFNHSLIQCEAARWDKHPEIT
                     APNAISKPDLRHLVELCRAHYQEPVPLINVPGHAEWIFRNHSNLDFAEDPQTPYAYCV
                     RHPHSFRFIRDVLKECLPIFQSQSCHIGHDEVTMRGRFPNPECPRCRDETVTTLVVEH
                     ANRLARWLERRGVQSMIWGDMLLGPGEASDATHAKTRAEAQQRRAQINKRITIVDWHY
                     AAAADDRSFKLLHEAGFRVIAASWYTPENIRRLSQAALQAGVEGLLQTTWMGFFPDER
                     ALESQPQQFAAFVLAAEYAWSGRADAPDQLGYDPGKVFQEACQHRLAGAATPRTP"
     gene            complement(11138..11560)
                     /locus_tag="PKI07_05910"
     CDS             complement(11138..11560)
                     /locus_tag="PKI07_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOC50623.1"
                     /translation="MSLYKTILVPMEGTPTDRAIIEHIKPLAKTMRSRVVLLHVASGV
                     PAKYHRTDAAGKEIEESKAWLEQVRAELNAAGIPASAELAYGEPAREIVKWVKRKGCD
                     LVAMSTHGHQFMADLVLGTTAIRVQHSLSVPVLMLRAR"
     gene            complement(11598..13031)
                     /locus_tag="PKI07_05915"
     CDS             complement(11598..13031)
                     /locus_tag="PKI07_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017814135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOC50624.1"
                     /translation="MSQPPPPPDSPAPAPPAAPAPGASGSHVSLEGIHSTVTVPPPHA
                     GFWRQWRAFVGPAILVSVGYMDPGNWGTDLAAGAQFKYGLLWVVGLASFMAVILQVIA
                     ARLGIATGKDLAQCCRDWFPAWTRWPNWLAMELAVAATDLAELLGSAVALNLLFHIPL
                     QWGILITALDVLLLLALQGLGMRMLEAVVAVFVATIGICYAIEIFALPQTQPDFAEMG
                     QALLQPGLRERGMLVIAIGIIGATVMPHNLFLHSALVQSRKLQRDEPSIRSAIRFNTL
                     DSAVALSLAFLINAGILVLAALVFYGKDSVTLPGGEMVRFTADSDWIRLAHLTLAPLL
                     GTSLASTLFAVALLASGQSSTITGTLAGQVVMEGFMRWRLRPWIRRMLSRLLAVIPAI
                     WIVSIRGTRSVTDLVNLSQVSLCLVLPLALFPLLIFTSSRRRMGPWANGWFLGLLGWG
                     SVLLITALDLYSLPESLQTAWRILLGR"
     gene            13398..14687
                     /locus_tag="PKI07_05920"
     CDS             13398..14687
                     /locus_tag="PKI07_05920"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC50625.1"
                     /translation="MPRDDQNAAPGLAAVPEETGATGLFPWRQTFAALRHRNYRLFFF
                     GQMISLVGTWMENTAQSWLVYQLTGSKLMLGTVAAVGSAPLLLFSTWGGSVADRHSKR
                     TVLLWTQSGMMLLAFVFAGLIWSGQVRPWHVLVLAALGGVAMAFDIPARQAFVVEIAG
                     REDLLNAVSLNSSLFNGARVVGPAVAGYLIAQVGMAMCFFLNGISFLAVLAGLMLMRL
                     PAFVPPAAPESTWQHALEGFAYVAKHRQVRTLLLLVAIVGVFGWSYSVLLPAFATDVL
                     RVGSREYGMLLSSNGVGAFCGALVVATYGRRLPLPRMIFGGLGFFALMLLLLAATQNY
                     YVALVFMGGAGWGMLLYLSTSNTVVQTSVADGMRGRVMGIWTLMFGGMMPVGGLEAGL
                     LSHWIGVPWAVAIGAMICGLAALAGWWQFHHRPPKPA"
     gene            complement(14938..16413)
                     /locus_tag="PKI07_05925"
     CDS             complement(14938..16413)
                     /locus_tag="PKI07_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOC50626.1"
                     /translation="MSKARAVIISAVILAVTLMSAQAQTAQTGATPAKLSATEQMIKD
                     IKNPTSWLNWGADLRIRNEYFNNLLTLNRDNPLHEQDYFRFRARLWATITPVEDLSFS
                     VRLAAEPREWMKDAGYTPMRTSPTTGRKGLDMREGIIDNLWVEYKNIAELPLTIKVGR
                     QDLIPGRDSLAGDGWLLADGTPGDGSWTYYFDAVRLKYEFKEQNTTFEAMGIAQDPRD
                     DGWLPTLNNQNLPQGDQREKGVVVQLANTSHKSANMAAYFIYKNDDRVSIHYPTVGDD
                     ADIYTIGARINGLLDDHWKYWIEGAYQFGRKADPRVQFPAGSSTASRDLSAYGVNARL
                     TYQFNDKLNNQLTLSGELLSGDDPKTRQDEMFDSLWGRWPRWCEIGLYGVAAEARIGQ
                     QANLLRLGPTWSIDPIKNRMRFVSSYYALFAMEKAATRGAPGLFSNDDHFRGHLAAAM
                     VEYTFGKHMKGHLWGELLFPGDYYEHQKMISFLRAEVMFTF"
     gene            17381..18325
                     /locus_tag="PKI07_05930"
     CDS             17381..18325
                     /locus_tag="PKI07_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOC50627.1"
                     /translation="MFAAVPSKRVCVMALSLAMLGAGCSLKRLAVNRVGDALAGGGMT
                     FAADDDPELVRAAVPFSLKLMESLLAESPRHQGLLLAACSGFTEYAYAFVQQDADELE
                     DQDFTAAEEMRARARRLYLRARNYGLRGLEARHRGLAQALRADARGALGGATVKDVPF
                     LYWTALSWAAAISLSKDNPDLIAELPQVEAMMDRALALDETFEHGALHTYFITYEMGR
                     SGAAGDPAARARAHFERALALSGGQLAGPLVSYAEAVCVQQQDLKEFTARLRQALAID
                     PGAKPEWRLANLVMQRRARWLLGRADQLFLRADPPPGN"
     gene            18352..19479
                     /gene="dctP"
                     /locus_tag="PKI07_05935"
     CDS             18352..19479
                     /gene="dctP"
                     /locus_tag="PKI07_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOC50628.1"
                     /translation="MNTPCLSVLFRMPRGAARSARARSLPGRARRGGPARRRAVLLLG
                     LAAAWVLAAPRLAAADKVVRLKLGTLAPAGTSYHKSVQMMGEKWRKESGGVVQLVVFP
                     GGTQGSEADMVGLMQTGNLDAAMITTEGLSQIDKDALALQIMPLAFRSLDEVDYLSEK
                     LRPKLEAKLAAKGYRVLFWCDSGWVRFFSKSPVVHPDDLRKLKVFSWAGNATQYDLWK
                     ASGFHPVALETSGIAQGFLSGTISAVPTVPIFALAAQLDTQARHMLEINWGPLVGAAV
                     VTQRSWERVPEAAREAMLKIADEAGRQIRAAGRAENDAAVAAMVKRGLVVHPMTPAAE
                     AEWLAVVDGVKDQIRGKVVPADVFDEAQRLLREHRAGGAGK"
     gene            19583..21460
                     /locus_tag="PKI07_05940"
     CDS             19583..21460
                     /locus_tag="PKI07_05940"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOC50629.1"
                     /translation="MSLESPASAEAHPGGGLTPRTGFRRVASQAEDLLLVVALAAMLL
                     LPVVEILLRHVFHTGISGSSAIVQHLTLIVGMLGGAVAARERRLLALSPIQTLLQGRA
                     RAAAQVFSSGFAAAVCLFLCVASLEYVLAVKPLGKILVYGLPVWVVQLILPVGFGLVT
                     LRLIWHATGTWRGRGLALALAAALAGLAAWGPVPPARLEIPGLVALAVATLLGAPVFT
                     ALGGAALLLFWGNDLPIQAVPLKHYSLTTNDLLPSIPIFTLAGYFLAESGASRRLVRV
                     FQALVGQFRGGPAIVTALVCAFFTSFTGASGVTILALGGVLMPVLLAARYSERHALGL
                     LTGAGSLGMLFPPCLPLILYAIVANTSAQANLSLKEIFLGGIGPGLLLVAITAWWGIR
                     KGSREAADRPAFRWAEARAALWDAKWELSIPVVAVGSLFTVPTIVEAAAVTATTALVI
                     AMVIHREVHPIRDLPRVITQCGLLVGGVLLILGVALGFTHYLVDAQLPDKLVAWATTA
                     VKSKWLFLLGLNLVLLFVGGLVEIYAAIIVVVPLLVPLGVAFGIDPIHLGIIFLANME
                     LGFLCPPVGLNLLLSSYRFNRPMAEVIRAAFPMLLVLLLGVLLITYFPPLTTFLPRLF
                     R"
     gene            21683..22180
                     /gene="msrB"
                     /locus_tag="PKI07_05945"
     CDS             21683..22180
                     /gene="msrB"
                     /locus_tag="PKI07_05945"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOC50630.1"
                     /translation="MQNFQKPSRAELRQKLTPRQFDVTQQAATEPPFRNEYWDHRAPG
                     LYVDVVSGEPLFSSLDKFDSGCGWPSFTRPVAAASVRERPDDSHGMRRTEIRSAAADS
                     HLGHVFDDGPAPAGRRYCINSAALRFVPVAQMEAAGYGAYLEPFIRAGLHPPAPARRD
                     PPAAR"
     gene            22261..22566
                     /locus_tag="PKI07_05950"
     CDS             22261..22566
                     /locus_tag="PKI07_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOC50631.1"
                     /translation="MLIVHVHVHVKPECVEAFKQASLANARESLKEAGIARFDCVQQQ
                     DDPTRFVLVEAYRTPQAPAAHKETRHYQAWRDAVAPMMAEPRSSVKYTNLFPEDAAW"
     gene            22594..23748
                     /locus_tag="PKI07_05955"
     CDS             22594..23748
                     /locus_tag="PKI07_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOC50632.1"
                     /translation="MRFEFASATRILFGAGTLREVGALARELGQRALVVTGASLIRAE
                     RLLHLLRGHGLISTIFSVTREPEMGVITQGVALARQQGCEMVIAFGGGSVLDAGKAIA
                     AMLTNPGELLDYLEVIGRGQALTLPAAPFVAIPTTAGTGSEVTRNAVLASPAHGVKVS
                     LRSPLMLPRIALVDPELTRDAPPAVTASTGLDALTQLIEPYVCTRAQPMTDALCEEGI
                     RRAARSLRTAFARGHDLAARTDMALASLFGGLALANAGLGAAHGFAGPIGGQFPAPHG
                     ALCAALLPHVMAANLDALRQRQPEAAALRRYDEVARLLTGQAAATAETGIAWVRELVA
                     DLRIPGLRQYGLKPEHIADLVRKASQASSMKANPIALTHEELAHILEQAL"
     gene            24165..24773
                     /locus_tag="PKI07_05960"
     CDS             24165..24773
                     /locus_tag="PKI07_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50633.1"
                     /translation="MKKSIIIATVLGVANMALAITSITTTYGPIAGPKGVNWDGYYAY
                     TWAVGAPALAADEVVIAATLSFNKINLTSGSGKLWVDFGTATASKSYVDFDIAGDYFD
                     GRAGFNAVDVGVQTWTKAGSKTWSWDITELADLNVRLHAGDLGFLFDPDCTFKVGSIS
                     FTYTTEKRGGGGGGGVPDGGMTAMLLGATLMGLGWAKRKISA"
     gene            complement(25063..29604)
                     /locus_tag="PKI07_05965"
     CDS             complement(25063..29604)
                     /locus_tag="PKI07_05965"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC50634.1"
                     /translation="MRRLKHRSTMRWRAAFLAAAGLAAAASVWAAPGTNNTGNSTVAV
                     GKQSVLATSDTPELTVLSPGQAASVTNNLAVYLSFDGHLNAQAGTTNHGTLYPGGAGP
                     GPRYAPGMIGAAASFTNASVSGQPGDWAVSLGNLEWIYSNSFSVSLWVRVTTNSDGAL
                     IGNKNWSGGANVGWVISSMASKNINWNAAGGPRRDLALSPAFSDGHWHLLAVTFNRPA
                     NEVIGYWDGVPVSSSNLGSSGAASLNAGLATLVGSSGSGAYSGSADVDDLGIWTRVLG
                     PREVSGIYAAGLRHLPLTAAVPGVPPVIIQQPASLAVTPGLDAVFSVDARGGGPLPYQ
                     WRRNGVNLPGATNATLVIPAATVDRQGAYSVRVSSGTGAVFSREASLTLYELKVTGQW
                     DFQQGDLRATVGADLEYAGNTAQQVSFPLKNIQGRVARVMAFGANGAGQGFYLRHGGR
                     PNGGGHFVNQYTLIMDVMFPAASSGQWRALWQTDPFNREGNEAEFYVSDAAAAPSPNG
                     IGAEGQFHGALAAGTWYRLAFAVNLAAPAGRQLMKYINGVLVGAQSLSGGVDGRFALG
                     PAVQLFTAGRPGLAQAGFVRSLQFVNGWMSPADIAALGAPTAEGLPPGNSVLKIKGLG
                     HNASTLELAWSGLGKDVQLQQAIRPAAPDWQNFLPATTNQQIHIPITAAQTRFRLQPA
                     PAEIQVGQLPEEQQSLPSRQILRAAGRQVQFAGRPVDLVLSPDGQTVYIKNMRNLLVV
                     DAAGWWIRQTLAFPGGGASMHGVAVSPDGSHIYATGAGNELYEWAVASNNLVSFVRTI
                     ALPGGSYPCGLAIAGDGSTAYVCLSISNTLAVVNLASGSVVRQINVGIAPWDVVLSPD
                     GNTAYVSDWGGRFPQTGDLTAPSAGTPVIVDSRGVAASGAVSVVNLSRGQETAQVSTD
                     LHPCDLELSADGATLYVANANSDTITVLDTRTRSVKETILVRPDPSFPYGSASTGLAL
                     SRDGQTLFVASGGNNAVAVVELPNARHAHSILRGFLPTDWYPGAVAADRDFVYVANVK
                     GLGSRLGQPAVTAWQISAHLGTANRIAIPDAEALAKYTAQVFEDGRVPQIRQTQRVLR
                     PGQPPVPVPARTGEPSVFQHVVYILKENKTYDQMFGDLPQGNGASNLCIYPRWVSPNH
                     HALAEEYVLLDNFYCNGVVSADGHSWSTEANVTDHLEKSFGGFTRSYTFGDDPLTYSS
                     TGFIWNNVLQHGLAFRNYGEMDYASTSPPKSWAEIYQDWLDGTGAIRYVQKIGLASLR
                     PYSSTNVPGWNLGIPDVVRADGFIKELRQAEATGVWEDFHFLYLPNDHTGGPPSPRAQ
                     VADNDVSLGRVVEAITRSVFGSNTVIFVIEDDPQSGYDHVDAHRSICLVISPYTRRGE
                     VISTFYNQAGVLHTMERILGLPPMNQHDAMAPLMFECFTNVPNFTPYTARPNNVPLDE
                     GVAATARPSRKQQYWAKQLAQMDFSKPDRINDDLFNRYIWWTIKGDQPYPARFVGGHG
                     RGLKQFGLKLVPPAPDDDD"
BASE COUNT         5174 a         9076 c        10097 g         5617 t
ORIGIN      
        1 agaatccagg gctgataaaa ggcggctttt tgagggtggg agggcgtctg gatggctctt
       61 gaatgtcttc tctgaaacgt tgacgttgcg tagttcagcc gtatgccttc gttccgctgg
      121 agccgagacg ggcctgataa gctcgtgact gagtatgcgg cgcagtgcaa gaaggtgctg
      181 gggcggttcg tgaacttcgt ccaggcgcag aatccgaagg cggtcgagat tgcgctggtg
      241 acgcgcaagc tcgcccaggc ttttatgggc gcggaggcgg agcgaggggt tggcgcgaag
      301 acctggaatg acacgctcaa gctgctccgc gccacgttca aatatctgct gcccgccggc
      361 gcggggaatc cgttttacaa catggtgacg aaggatgccg agacggtctt ccgcaagccg
      421 ttcacgccgg aggaactgaa ggccattctg gacgcggccc aggatgatga tttcatccgg
      481 cccattatca tcaccgggat gtgcaccgcg atgcgccggg gggactgctg ccggctcaag
      541 tggaaggatg tggatctggc gcggcggttt atcacggtga agacggccaa gaccggccag
      601 acggtgagca tccccatttt tccgctgttg ctgggcgagt tggccagcct gccgacgcag
      661 ggcggggaac tggtgttccc ggagcaggcg gagatgtata agactgatag gaaattgctt
      721 tttacgcgcc cgcatgtact cagcgctgcg tgggtgatag tttccgtgac aggccgcgct
      781 cgctccccaa cacttcgggc acggtgtagt tagtccggag cgttttcgtc gtggccagat
      841 ggtccacgtt gagcaaaact ccatcccgca tatcgccaat gggaatggtc tggcggtcat
      901 gaaacattct gccaccgcat ccggtcactc tctggcgcag ttccagcgtc aggacggagc
      961 ggttccggct ttgcccgagt cggattccgt attccggcgc gggcaggcgc acgaggattt
     1021 catcccgcaa taccccaccc ggactggcca cgggcaatcc gacgtattcg acaagcacgt
     1081 tccacaacgg ctcgcggatt ttgttgtaaa cgtagttcaa gcagttgcgc tcatatacgc
     1141 gctgatggat ttgtggcatg aaggaaatca tagctcagta tccttactga taggaatttg
     1201 ctttttgcgc gcccgcatgt actcagtgct gcgcgggtga gctcatcgcg gcgcgcgaat
     1261 tccagcggca gccgcggctg ctgatcgcgg cccagcccac gcgcggggtg ggcgttggcg
     1321 ccattgagtc cattcacaca cgcattgtgc gcgcccgcaa cgaaggcgct ggcgttctgc
     1381 tcgtgtcctc cgaactggac gaagtcctga ccctgtccga ccgcatcctc gtaatgttcg
     1441 aaggtcgcat ggtgggtaaa ttccgccgtg gcgaagtcac cgagcgcgaa ctgggcctca
     1501 agatgggagg ggcgtgaatt ggagggatag tggagccgat gggcgccgaa tgcctggccg
     1561 cctgaatgcc atcgggttgc gagtgcaagc aggcgtgctg tttatcatgg caaaggtgca
     1621 gccgtcagat ggcgtctaat gcccttactg gctttctcca aggcgtattc gccttgtatg
     1681 cctggaaggc ggaggcagcc acaactccgg agggtgatcc ggtgtcacgt gccgctgtgg
     1741 cattcttgcc ttcatcaacc tttcggggtc gggaggcctc gcgcagcatc aaacctggcc
     1801 tgttcactcc ggaggatgtt agtcgcttaa cgaaaaaatg tcgcgcaaaa tgcgcaacat
     1861 tttccgtgca atcgtgagtc gggttatgca tgatgacgac gctcagattg agcaatgttt
     1921 acgcgcctct ggcgctcgtt cacctgcaag cgcaggaaca tcggatggca atgtgagaag
     1981 gcaaacattg ccgtcactct tgacaggccg atggctatgc atacctggat tttcagattt
     2041 taactcttgg ttccggctac gccgggttag gtatcaatat agtataaaga ttaaatattt
     2101 actaaatgcg ccgagtttat cacaggccaa aacctgagtc agacatgaaa aaatttaggg
     2161 ttaatcagca gatccgcgtg ccggaggttt ttttaattga tgaaaacggc cagagcattg
     2221 gcgtcatgcc gacagccaaa gctttaagct tggcgctaga agccggttta gatgccgtcg
     2281 aggttaaccc taaagttcaa ccgcccatag ttaaaattat agattacggg caatgcagat
     2341 ataaaaaaga gaaagaagcc cagaaacaga aagtcacccg cgcagcgctg agtacaggtg
     2401 ggcgcaaaaa gcaaatcagt tccgggcgct ccgagtccaa gacggcggta caaaaggctc
     2461 tgtcgtgtcc gttccgctgc cgtcttaaac tgagccgcgc actgatgatt gctgcggctt
     2521 tcagcccggt cacatccttg gctggttggc gcaggattct ttcgcggcgt tggttgaaga
     2581 aacagtggcg gattcttcaa tgccgcatcc gccgacgcct gttttgcagt gcaacagtca
     2641 ttgccgtcac gggaagttgc ggcaagacca gcacaacgca ctttttgggc aagattctct
     2701 ccgccgaaca ctcatgccgc atcggcatct cagataatgg tcaggaagca atgctggaaa
     2761 acttgcgccg gttaaaccgt tcacatcggt ttgtgctcca ggaaacaggg gtcaatggcc
     2821 cggggtcttt gaacgagata gtgccgatcc tccgtccgca tatcggaatt gtgacgagca
     2881 tcggccagga tcattaccgg aacttccgaa cattggaagc gactgccgcc gaaaagggga
     2941 ttttgatcga atctcttcct gattcggggg ttgccgtact caatgcggat gatccatatg
     3001 tgattgcaat ggcctctaga accaaggcgc gcatactgac ctacggtctg tccgacaggg
     3061 cagatgttcg cgccaccgat atacgcagta catggccgga gcgcctttca ctgactgtga
     3121 cctatcaggg tacatctatc cgtattgaga ctgggctgtt cggaacactg ctgtccaact
     3181 ctgttctggc agcggtttca ggagcccttg cagcaggcat cggcctgaag cagtgcgccg
     3241 ctgcgttgaa atccgtcgag acattctcac gaaggatggc gatccactcc acatcccggc
     3301 gtgtctggta tatcttggac acggtaaaag ccccctattg gagtatcggt aaagttcttg
     3361 cgcttatggc cgatgttgat gcccccagaa aaacagtcgt gatcggttct ttttccgacg
     3421 ctccgggatc ggattcagac aagtaccgct gcctagctag agacgccttg aacattgcgg
     3481 atcgggtact ttttgtcggt cgcaaggcta tccacatcaa taagatgctg actgctgaga
     3541 cgcatggccg cctttttgcc atggaatccc tccaggaagc cgccaaactg ttggctgacg
     3601 atgttgtggc cgacgaggtc gttttgataa aatccggaaa cctggagcat gctgaacgtc
     3661 tgtatttcag ccaggacaac atcttgaggt gctggaagcc aagctgccgc ttccgctcca
     3721 gctgcgagta ctgcacggaa agtgggctgt caggataaac taactactga taggaaattg
     3781 ctttttacgc gcccgcatgt actcagcgct gcgcgggtga gggagaattg gtttttgagc
     3841 tcaccaagca tgtggcggcc ttatcaggag ccggctggga cgtcaacgca tttatttgcc
     3901 ttttcttgcg gcaatttttg cccacctgga agctcccaac ggcccatctt gggcgcggag
     3961 ggtgtcgcgg ggtgtgaatg gagcccaaag ggcccggtgc atgggcgcac ctcaccccga
     4021 gccgtctgag gcggcggcag ttgtttcagt cggtcagcac gttctcgtag tcgggcatgg
     4081 ggtccacgtc gtcacagggt tcgtaggtgt agggccccgg cacccgctgc ggaggcggcc
     4141 tggggggagg gtcatgccag gcgccgaggt ggcggagaat cttctccgcc acccgtcggt
     4201 catcaatgac ggcgatgacg cgcatggggt tctggcagac ggggcagcgg agcgggttca
     4261 tgtgccagac gtgcagaata agggcgcgca gcgtttggtg ggaagcttga ccgacggcgg
     4321 cgaatgtggt ggcgctgccg acagaggcgg cggtatgagg ggcaggcgcc cgcacccgtc
     4381 gcgcgtgttt caaccgcgtc gcgtgcggct acgcaaccag aagaggatgc cgccaacggt
     4441 tagcggggct gaagcggatg gctcggggat ggcgccgcca ggatggaggc atcggggcgc
     4501 gtatcccacg cccacgcttc aatgcttcct gccggataat cggaagcgac attcagcagc
     4561 acccaccata atgcgtagcg ccgccgcatc cgccagttcc gccgccgggg cgacgggtgg
     4621 atgctgcgtt tcctccgcgt gcgctgcaac cgctgggacg agctccagcg cctgcgccgc
     4681 ttcggcgtgc cccaagtcac cccccgctga acgggtgccg ggcaaaaatc tccggcggcg
     4741 cggccgtccg gggcgcccgc ggctaggacg tgggcggcgg atcggcggcg ggaggttcgc
     4801 ccatcgcttc cagccgcttc tccagttcct tgacccggcg cagcagctcg ggcagttgct
     4861 gcaaggcgat catctgccgc ttggcctggc ggtcgggttg ggcgggcgtc cagagccact
     4921 tttcgccgtc ggggatattg tgcattacgc cggactgggc ggcgaccgcg acgcggttgc
     4981 cgatgcgcaa gtgcccggcc aggccgacct ggccgccgag caccacgtag ttccccagcc
     5041 gggtgctgcc cgagatgccc acttgcgaaa cgatgatgca atgctcaccg agcgtcacgt
     5101 tatgcgcgat ttgcaccagg ttatccacct tggtgccccg cccgataaag gtcggcccca
     5161 gcgccccgcg gtccaccgtc acattcgccc ccaattccac gtcgtcgcaa ataatgacat
     5221 tcccaatctg cggcaccttg cggtggacgc cgttgtccag cacgtagcca aagccgtccg
     5281 cgcccaccac ggtgccggcg tgaatccgca cgcggtggcc gacctcggtg cgcgggtaaa
     5341 gggtgacgtt ggggaacagg ttgacttcct cgcccagcac gcagttcgcc ccgatatgat
     5401 tcagcccctg cagcacggag cgcgccccga tccgcacctg ctcgcccagc acgcagtaag
     5461 gcccgatgtg ggccgtggga tccacctggg cgctcgccgg caccagggcc gtggggtgga
     5521 tgcccggcgc gaagggcgtt tccgggaaaa acaaggggag cactttggca aacgccacgc
     5581 gggcgttggg cacccggatg agcgtcttgc cgcgcgaggt ataggcgctg tccaccagga
     5641 tggccgacgc cgcgctttgc tcggcgcggg caaagtaatt cgcgttttcg gcaaacgtca
     5701 ggtcgcccgg ctgggaacga tccgccggcg caaacccgtt cagcaccagc gacgcgtctc
     5761 ccgtgatttc acccccggtt aatcgggcaa tgtcagctac agtataaggc ataagcggtt
     5821 gactgggaca acgcgggcca ttataaaaac cacggccgcg cattcaacct ttaactgcgg
     5881 gttgaagcag ccggggcgtt cgggccgtga gcccgggccg cccgcccggc aggctgcgga
     5941 tccattcggg cggcaaaaac aaacagtaac aaagacacaa tccaggagca tcaatatgtc
     6001 gaacagacct ctgaatgtcg gtttagtcgg cggcggcaaa ggcgccttca tcgttcaccc
     6061 ccaccagcga gccattcact ttgacggcac gcggcgcgtc gtcgccggcg cgctcttccc
     6121 cgatccccgg atcgcgcgcg ccgaagccgc ccagtggccc tatcccctca agggctacgg
     6181 ttcctacgat gaaatgatcg ccgccaacgc ccaactcccc gaagccgaga agctggacta
     6241 catcctgatc gtcaccccca acttcgtcca cttcgacccg gcgatgaaag ccatgaaagc
     6301 cggcatcgcc gtgttctgcg aaaagcccct gtgcctgaac ctcaaggagg ccgccgccct
     6361 ggtgaaaacc gcgcgccagc tcaacatccc cttctgcgtc gcccatacct acgtcggcca
     6421 ctggaccagc cgcctgagcc gctatatcgt ccgcagcggc ctgctgggcg aggtgcgctg
     6481 ggtggatagt tcctacctcc agggctggct ggcgacccgg ctcgaagcca ccggccagca
     6541 acaggcctcc tggcgcgtgg accccaaacg cgccggcggc tccggctgcg gcggcgacat
     6601 cggcacccac gccctcatgc aactccgcta cgtcaccggc cttgaggtca ccgcgctgag
     6661 cgcccagctc gagaccttcg tccagggccg ccagttggac gaccacttca cgatctattg
     6721 ccagctcagc aacggcggca aagccctcgt ccgcgcctcc caaatctgca tcggctacaa
     6781 gaacgacctc ggcatcgtcg tcgccggcac caaaggcacg ctttgctggc gccaggaaga
     6841 acccgagtcc gtcaccatca agctccccgg ccagcccgac cgcgtctatt ggcgcggcgc
     6901 ggtcacgccc ggcgacggct tcctgcccaa aaacgtcccg gcggatctca tggccgagcc
     6961 gaccatcccc tcgggccatc ccgaagcctt ccacgacgcc ttcgcgcgcc tgcatcgctg
     7021 cttcgaggcc gacgtgcgga agtggaaagc cggcaaagcc ttcaagtgcg acggcgccaa
     7081 atacgccaac gtcgaagacg gctggaccgg cattgccttc atcgaaacct gcgtcaagag
     7141 cagcaaaaag aagggcgcct ggacgcccat gcccaaagcg atctaaccgt cgcccggcac
     7201 aaatctctgg gccagcccgg gccgggagcg cggccgctcc ggagaacccg ggttgcccgg
     7261 ctttaagaaa tgagggagcc ggcgcggcct cgcccaatgg cgcaccccgc aaccggccac
     7321 cccgcccgcc tcgcgccgcc gctgccattt acacctgccg ggtgaatcgg aggcgctccc
     7381 cggcgccata gccggccacg cccctcgtct tccctcccat tcccgcgggc cgggatcatt
     7441 gcgcggacag gttcatccgc cgcccgcccg cttcgccgtt tatcggcggc aaatcctcct
     7501 gccaattatt ttatccgtac ttatacggaa ctgacggtaa ttgtcctacc catggttcta
     7561 agctgggcga aaacaaattg aacctatgac gataacaaac acgcaactgg aactgagctt
     7621 gggaaaccgt caaacccatc ggctgccgtc cgccacgtcc aattcgtccg gccaccgcgc
     7681 ccagtggtgg ttccggcgga tgcggcagat cgtggactgt gcgctggact ggcagcccgc
     7741 ccctccgccc cggccgcagc agacttcctt cgcaagcatg gatttcagcg cctggacggc
     7801 ctgagttcat tccaccgcca cacgcctggg ttagcgtgac cgcgagtcaa taaccctccg
     7861 gcagctttct tcgctgccat ccttccctca cgtccgggcc tggcccggac tgtccattta
     7921 ttggacactc tcccattccc agcttccggc aacccctttt ctcccttcgc ccgccagaaa
     7981 gccctttcac cgcctaccgc agcttctggc cgccttcggg ctgctttcaa gccgtgtaca
     8041 aggggtgtac aacgggtgta ccaggggtgc ccaccggaaa aacaagggaa aatccgtagt
     8101 acatccgggg tgcatccgtt gtacacccct tgtacacggc gcattgggat ggggagaatg
     8161 gcgggattgc gggacgggat gggctggctt ttgggctttt ggggggagcg gaaaggggcg
     8221 gcggcggggc ggcgggaagt tcgtccttga cggaaggggg ttatccgggt gcgatagagg
     8281 cagactgaga acaatcgcaa tcgcagatac accttactat gagcaatcaa ctggcaatta
     8341 acggcggccc caaggccgtg accaataaac tgcagagctg gccccagttc gacgaaaacg
     8401 ccattaaagc ggtcgagaca gttttacggt ccggcaaggt gaattactgg accggcaagc
     8461 agggcatgga gttcgagaag cagtttgccg cgtggcaggg cagcaaatat gccgtcagcg
     8521 tggccacggg cacggcggcg ctgcatgtcg cgcttaattc gctgggcatc ggccccgggg
     8581 acgaggtgat cgtgccgagc tatacgttta tcgccagcag cttttcgatc gtgcaggcgg
     8641 gcgcgattcc gcggttcgcg gatgtgaacc tggaggatca ctgcctcagc gtggcgtcgg
     8701 cggagaagct ggtgacgaag cggacgcggg ccatcatgcc ggtgcatctc tatggcaacg
     8761 tttgcgatat ggaccagatt caggcgtttg cccggcggca taacctgtat gtgatcgagg
     8821 ataacgccga ggcgttcggc ggcgtgtata agggccgcaa gaccggcacg ctgagccacc
     8881 tggccggctg cagtttttgc cagaacaaga cgttcaccac cggcggcgag ggggggatgg
     8941 tgacgacgga cgacgaggac ctggcctgga aggcgcgcag tttccgcgat catggctacg
     9001 atgtgaagca gcggttgagc ctgctcgaac tggagcagaa gctgccctac atccataaca
     9061 tggtgggctg gaactaccgg atgacggaga tgcaatcggc gatcggactg gccgagttgg
     9121 cgcgcatgga tacctggaac ctgccgcggc ggcggcgcaa cgcgcagatc ttaatcaatg
     9181 cgctcaagac gtcggagtat atcaagtatt ttcccattga tacgccggaa cggcaaaacg
     9241 ggtggtatgt gctggccttc tcgctggagg tggaacggat gaagtgcgac atcaagcagt
     9301 ttgtcgccgc cgtcgccgcc gagggcgcgc cggtctggaa agttttctgg ccccagtgcc
     9361 acaccgagcg ggcgtttcag gagcaccatg cctttggccg caccgggttt ccgttcaagt
     9421 cccgggaata tgcggacccg gcgagtgtgg actaccggga ggtggaggtg cccaatgccc
     9481 gctggcatga ggcccacacg ttcacgtgct ttgcctatcc cacctttacc gagtccgaca
     9541 tggagcagat cgccgaggcg atcctgaagg tgatcaaggc gtacgcggcg tagcggcgcc
     9601 ggcgagccgg tgctggcagg cttcctggaa gaccttgccg gggtcgtacc cgagctggtc
     9661 gggcgcgtcg gcgcgccccg accaggcgta ttcggcggcg agcacgaacg cggcgaactg
     9721 ctggggctgg ctttccaggg cgcgctcgtc ggggaagaac cccatccagg tggtctgcag
     9781 gaggccttcg acgccggcct ggagggcggc ctgggagagg cggcggatgt tttccggcgt
     9841 gtaccaactg gcggcgatga cgcggaagcc cgcttcgtgc aggagcttga agctgcggtc
     9901 atcggcggcg gccgcgtaat gccagtccac aatggtgatc cgcttgttga tttgcgcgcg
     9961 ccgctgttgg gcttcggccc gggttttggc gtgcgtggcg tcgctggcct cgccgggccc
    10021 gagcaacatg tcgccccaga tcatgctttg cacgccccgc cgctccagcc accgggcgag
    10081 gcggttggcg tgctcgacca ccagggtggt gactgtttca tcccggcagc gcggacattc
    10141 cgggttgggg aagcgcccgc gcatggtgac ttcgtcatgg ccgatgtggc aggattggga
    10201 ctggaagatc ggcaggcatt ctttgagcac gtcgcggatg aagcggaagc tgtggggatg
    10261 gcggacgcaa taggcgtagg gggtctgcgg gtcttcggcg aagtccaggt tgctgtggtt
    10321 gcggaatatc cattcggcgt ggccggggac gttgatgagc gggacgggct cctggtaatg
    10381 ggcgcggcac agctcgacca ggtggcgcag gtccggcttg gagatggcgt tgggggccgt
    10441 aatttccggg tgcttgtccc agcgggccgc ctcgcattgg atgaggctgt ggttgaattt
    10501 caacgcgctg aagacgttgg tgatgagccg ctggtgcatc ggcgcgccgg aggcgctggg
    10561 aaaccagtgc gccccgcgaa agcgcagggc cggccagtcg tccacttcga gcgccgggca
    10621 ggtgcgcgcg cggccggcgg gcgcccaaag ctgcgccagc gtttgcaggc cgtagaaggc
    10681 cccctgcgcc gttgaggcga ggatggccag cccgccgggt tccaccacca gccggtagcc
    10741 ctcggggtcc tgcgcccagc gggcttgccc ggcggggaaa acagtctggc ggggcgagcc
    10801 gatttgcagg acgacgcccg gcgagcggct gcgggtgaag gcgcccgggg cgccggggaa
    10861 cgcggcttcc gccgcctgcc agagccgcgg gtcgcgcgcc gccgcccgga gggtccattt
    10921 cattcccggt tccagccgga aggttcccgc gcccaggcgc agcgatttgg gctgggggat
    10981 gatgagcggt ttgccgggcg cggagacggg agccgccgcc ggggccggcg gggccgccag
    11041 caacagcggg acgcagagac cgatggacag cagggagacg cagtatttca tgatgcggcc
    11101 gtttatacgc caacggcgcg gcaggcgcac gcccgaatca ccgggcccgc agcatgagga
    11161 cgggcacgct gaggctgtgc tggacgcgga tggcggtcgt gccgagcacc aggtcggcca
    11221 tgaactggtg gccgtgcgtg ctcatggcga ccaggtcgca gcccttgcgc ttgacccact
    11281 tgacgatttc cctggccggt tcgccgtagg cgagttcggc gctggccggg atgccggccg
    11341 cgttgagctc ggcccgaact tgctccaacc aggccttgct ctcttcgatt tctttgcccg
    11401 cggcgtcggt ccggtggtat ttggccggca cgccgctggc gacatggagc aggacgaccc
    11461 ggctgcgcat ggttttagcc aggggcttga tgtgctcgat gatcgcccgg tcggtgggag
    11521 taccttccat ggggacgagg atggtcttgt agaggctcat aggttgacag gagttaaggg
    11581 atgggatgca tgggagatta tcggcccagg aggatgcgcc aggcggtctg gagcgactcg
    11641 ggcaggctat agaggtccag ggcggtaatc agcagaacgc tgccccagcc caggagcccc
    11701 aggaaccagc cgttggccca tgggcccatg cgccggcgcg aggaggtaaa gatcagcagg
    11761 ggaaacaagg ccaggggcag gacgaggcag aggctcacct ggctgaggtt gacgaggtcg
    11821 gtcacgctgc gggtgccgcg gatgctgacg atccagatgg ccgggatgac ggccaggagc
    11881 cgggacagca tgcgccggat ccaggggcgc aggcgccagc gcatgaagcc ctccatgacg
    11941 acttgcccgg ccagcgtgcc ggtgatggtg ctgctttggc cgctggccag gagcgccacg
    12001 gcaaagaggg tgctggccag gctggtgccc agcagcgggg ccagcgtcag gtgcgcgagg
    12061 cggatccagt cgctgtccgc ggtgaaccgc accatttcgc cgccggggag ggtcacgctg
    12121 tccttgccgt agaagaccag ggcggccagc acgaggatgc cggcgttgat gaggaaggcc
    12181 agcgacaggg ccacggcgga atccagcgtg ttgaaccgga tggcgctgcg gatggagggt
    12241 tcgtcgcgct gcagtttgcg ggactgcacc agcgccgaat gcaggaagag attgtgcggc
    12301 atgacggtgg cgccgatgat gccgatggcg atgacgagca tgccgcgctc gcgcaggccg
    12361 ggttggagca gggcctggcc catctccgcg aaatcgggct gggtttgcgg cagcgcaaag
    12421 atctcgatgg catagcagat gccgatggtc gcgacgaaga ccgccaccac cgcttcgagc
    12481 atgcgcatcc ccagcccttg cagggccagc agcagcagca cgtccagggc ggtgatgagg
    12541 atgccccatt gcagcgggat gtggaacaac aggttcagcg ccaccgcact gccgagcagt
    12601 tcggcgaggt ccgtggcggc gacggccagt tccatcgcca gccaattggg ccagcgagtc
    12661 caggctggaa accaatcgcg gcagcattgg gcgaggtctt tgccggtggc gatgcccagc
    12721 cgcgcggcga tgacctggag gatgacggcc atgaagctgg ccagccccac cacccacagc
    12781 aggccgtatt tgaactgcgc cccggccgcc aaatcggtgc cccagttgcc cgggtccata
    12841 taacccacgc tgacgagtat ggccgggccg acaaaggcgc gccactgccg ccagaaaccg
    12901 gcgtggggcg ggggcacggt gaccgtgctg tggatgccct ccagcgagac gtgggagccg
    12961 gacgcgcccg gcgccggggc ggcaggcggc gcgggcgcgg gcgaatcagg gggcggcggg
    13021 ggttggctca tgacgcgccg ttatcgcccg gtttccccgc gcggcgccgg cccttgccga
    13081 gcgttgcttc ccagcttttc catgattcgc ttcttagcgt gccgtggggg tgaagtcaac
    13141 caccgcttgc acggcgcggg gagggcgagg tttcgccggc cgcattccgc gctggcggcc
    13201 ggggcgcaaa ttgcggtagc ctgaccgccg gtctggtcgt ttccgtggcg aaaacgccgc
    13261 ggcgggccga agctatgtca aacgatggca aacattgcgg gataaagcgg gaagaaccgc
    13321 gggaagcggg ctgggaaaac cccgcacccg cgcgcccgcg cccggtgggc ggttcgcggc
    13381 ggttccggta gcaggccatg ccgcgcgacg accagaacgc cgcccctggt ttggcagcgg
    13441 tgcccgagga aaccggggcg acggggttgt tcccctggcg gcagacgttt gcggcgctgc
    13501 ggcatcgcaa ctaccggctg ttctttttcg ggcagatgat ttcgctggtg ggcacctgga
    13561 tggaaaacac ggcgcaaagc tggctggtgt atcagctcac cggttccaag ctgatgctgg
    13621 gcaccgtcgc cgcggtgggc tccgcgccgt tgctgctgtt ctcgacctgg ggcggttcgg
    13681 tggcggatcg gcattccaag cggacggtgc tgttatggac gcagagcggg atgatgctgc
    13741 tggcgtttgt gtttgccggc ctgatttgga gcgggcaggt gcggccctgg cacgtgctgg
    13801 tgctggcggc gctcgggggc gtggcgatgg cgttcgacat cccggcgcgg caggcgttcg
    13861 tggtcgagat cgccggccgg gaggacctgt tgaacgcggt ttcgctcaac agctcgctct
    13921 tcaacggcgc gcgggtggtc gggccggcgg tggcggggta tctgattgcg caggtgggga
    13981 tggcgatgtg cttctttctg aacgggatca gctttctggc ggtgctggcc gggctgatgc
    14041 tgatgcgcct gccggccttt gtgccgcccg ccgcgcctga atccacctgg cagcacgcgc
    14101 tggaggggtt tgcctacgtg gccaaacacc gccaggtgcg cacgctgctg ctgctggtgg
    14161 ccattgtcgg ggtgttcggg tggtcctact cggtgctgct gccggcgttt gcgacggacg
    14221 tgctgcgcgt cggcagccgc gaatacggga tgctgctcag cagcaacggg gtgggcgcgt
    14281 tttgcggggc gctggtggtg gccacttacg gccggcggct gcccctgccg cggatgattt
    14341 tcggcggcct ggggttcttt gcgctgatgc tgttgctgct ggcggcgacg cagaattact
    14401 acgtggcgct ggtgttcatg ggcggcgccg gctggggcat gctgctttac ctgtccacca
    14461 gcaataccgt ggtgcagacg agcgtagccg atggcatgcg cgggcgcgtc atgggcattt
    14521 ggacgctgat gttcggcggc atgatgccgg tgggtggatt ggaggcgggc cttctttccc
    14581 actggatcgg cgtgccgtgg gcggtggcca ttggcgcgat gatttgcggc ctggcggcgc
    14641 tggcgggatg gtggcagttt caccaccgcc cgcccaaacc ggcatgaacg ccgcccgccc
    14701 ccggccgctg gcttccggca gggccagtgg atcgcccgcg ccgaggatac gaactccggg
    14761 ctttggcgga tgcttgtccg aggctggcca ggccgttaac gcttgccgcc ggccctgaag
    14821 aagcggcgcc ccgtacgtat taaaagccgg caccgcgaag gtgccggctt gttttcaaca
    14881 actgccttca gccccgcgcc gccgcgggcg gcgccattcc ccacgcgcgt ccgcgcgtta
    14941 gaaagtgaac attacctcgg cccgcaggaa ggaaatcatc ttctggtgtt cgtagtagtc
    15001 gccggggaag agcagttcgc cccagagatg acccttcatg tgcttgccga aggtgtattc
    15061 gaccatcgcc gccgcgaggt ggccccggaa atggtcgtca ttgctgaaga ggccgggtgc
    15121 gccgcgggtt gccgcctttt ccatggcgaa caaggcgtag tagctggaga cgaagcgcat
    15181 ccgattcttg atcgggtcaa tggaccaggt ggggcccagg cgcagcaggt tggcctgctg
    15241 gccaatgcgc gcttcggcgg cgacgccgta aaggccaatt tcgcaccagc gcggccagcg
    15301 gccccacagc gagtcgaaca tctcatcctg cctggtcttc gggtcgtcgc cggagagcag
    15361 ctcgccggag agcgtgagct gattattcag tttgtcattg aactggtagg tcagccgggc
    15421 gttgacgccg tatgccgaca ggtcgcgcga ggcggtgctg gatccggccg ggaactggac
    15481 ccggggatcg gctttgcggc cgaactggta ggcgccttcg atccagtatt tccagtgatc
    15541 atcgagcaaa ccgttgatcc gcgcgccgat cgtataaata tccgcgtcat cccccaccgt
    15601 cgggtagtgg atcgaaaccc ggtcgtcgtt cttgtaaata aagtaagcgg ccatattcgc
    15661 ggacttgtgg gaggtgttgg cgagttgcac caccacgccc ttttcgcgct ggtcgccctg
    15721 cggcaggttc tggttgttga gggtcggcag ccagccgtca tcacgcgggt cctgggcaat
    15781 gcccatcgct tcaaaggtgg tattctgttc tttgaactcg tatttgagcc gcaccgcatc
    15841 gaagtaatag gtccaggaac catcccccgg cgtgccatcc gccagcaacc agccgtcgcc
    15901 ggccagcgag tcccggcccg ggatcaagtc ctgccgcccc accttgatgg tcaacggcag
    15961 ttccgcgatg ttcttatatt ccacccacag attgtcaatg atgccctccc gcatatccaa
    16021 ccccttccgg ccggtcgtgg gcgaagttcg catcggcgta tagccggcat ctttcatcca
    16081 ctcgcggggc tccgccgcca gccgcaccga gaagctcagg tcctccaccg gggtgatggt
    16141 ggcccagaga cgcgcccgga accggaaata gtcctgttcg tgcaatgggt tatcccggtt
    16201 caacgtcaac aggttgttga agtactcatt gcggattcgc aggtcggctc cccaattcag
    16261 ccaggaggtt ggatttttaa tatccttaat catctgctcc gtggcggaga gctttgcggg
    16321 cgtggccccc gtttgcgcgg tttgcgcctg ggcggacatg agggtaaccg ccagtatgac
    16381 ggcggatatg attactgcgc gagccttact catttttggt cgttcctttc aagtcttgct
    16441 gcttttagtg gaagcgagca ttgatcagtt gtttaagttt caggttcttg cctgcattgc
    16501 ggggttggtt tgcattgctt tccgcaccca caatacaacg gcccggattt tatggtttta
    16561 ggcctgaatg tccagctttt tgcaaagatt tttttcggcc acgccattgg gctaccagcc
    16621 cgtctaattc catgataggg caaagttatc tcctttctga cagtaaaatt aattatactt
    16681 ttcgccagtc aggcccgaat ccgagaccat gaacccatta aaaccgggga agaatactgg
    16741 gcgccccagt cttgaaccca attcaacccg ccagcctggg aaccggcggc cgtttcagac
    16801 tccaacaatc acccgataaa catccaggga atccacatgg agaaggcggg catcattgat
    16861 atccccgatt tcagtgccca aaaaagacag gccgcataga gcctcgcgcc gctcccaaac
    16921 cgtcctgcca gggccatgcc cgattcccaa cgccgttttc cgtttaaccc ccgtcattcc
    16981 agccacatat aaccctacca tcaagccatg tacaaggggt gtacaaggga tgcccaccgg
    17041 atgttcatca gattttccga tgttcatccg gcgagcatcc cttgtacacc ccttgtacac
    17101 ggctatttgg gagggggaaa ggagggggat gggaggcgga agtgaagatt gggctgggaa
    17161 aagggttggg gggccgggga ggcaggctgc caatgggttg gggagtcttt atcccgggag
    17221 gctccggggt ggcgggcggg gggtggttgc cggggccgag ggtgttggcg ttcgttttgg
    17281 gtggggtgga ctggacgcgg ttggcgggtg ggggaattat ttttcgcgat tggcatccgg
    17341 ggcgggaatt ggcatacatg ggcgcgaaat caatcaaccg atgtttgccg ccgttccaag
    17401 caagagagtt tgcgtgatgg ccttgagcct ggcgatgctg ggcgcgggct gttcgctcaa
    17461 gcgcctggcc gtcaaccggg tgggcgatgc cctggcgggc gggggcatga cgtttgccgc
    17521 cgatgacgac ccggagctgg tgcgggcggc ggtgccgttc agcctcaagc tgatggagag
    17581 cctgctggcc gaaagcccgc gccatcaggg actgctgctg gcggcgtgca gcgggttcac
    17641 cgaatatgcc tatgccttcg tccagcagga tgcggatgag ctggaggacc aggactttac
    17701 cgccgccgag gagatgcggg cgcgcgcccg gcgcctgtat ttgcgggcgc ggaactacgg
    17761 cctgcgcggg ctggaagcgc ggcaccgggg tttggcgcag gcgctgcgcg ccgatgcccg
    17821 gggcgccttg ggcggggcga cggtgaaaga cgtgccgttc ctttactgga cggcgctttc
    17881 gtgggcggcg gcgatttcgc tgtcgaagga taatccggat ttgatcgcgg agctgccgca
    17941 ggtcgaggcg atgatggacc gcgccctggc gctggacgag accttcgagc acggcgcgct
    18001 tcacacttat ttcattacct acgagatggg ccgttcgggg gcggcgggcg atccggccgc
    18061 ccgggcgcgg gcgcatttcg agcgggcgct ggcgctcagc ggcggccaac tggccggccc
    18121 gctggtttcc tacgcggagg cggtgtgcgt gcagcagcag gacctgaagg aatttaccgc
    18181 ccggctgcgc caggccctgg cgattgatcc cggggcgaag ccggagtggc gcctggccaa
    18241 tctcgtcatg caacgccgcg cccggtggct gctggggcgc gcggatcagc tcttcctgcg
    18301 cgccgacccg ccgccgggca actaacgttt ttacctggag tttaattact gatgaatacc
    18361 ccttgcctgt ctgttttatt ccgaatgccg cggggggccg cgcgatcggc ccgcgcccgc
    18421 tcccttcccg gccgggcgcg ccggggcggc ccggcgcggc gccgggcggt gctgctgctg
    18481 ggcctggccg ccgcgtgggt gctggcggcg ccccggctcg ccgccgcgga caaggttgtc
    18541 cggctgaagc tgggcacgct cgccccggcc ggcacctcct atcataagag cgttcagatg
    18601 atgggcgaga agtggcgcaa ggagtcgggc ggggtggtgc aattggtggt gtttccgggg
    18661 ggcacgcagg gcagcgaggc cgacatggtc ggcctgatgc agaccggcaa cctggatgcg
    18721 gcgatgatca cgacggaggg gctgtcgcaa attgacaagg acgcgctggc gctgcagatc
    18781 atgccgctgg cgtttcgcag ccttgacgag gtggattacc tgagcgagaa gctgcggccg
    18841 aagctcgagg cgaagctggc ggccaaggga taccgggtgc tgttctggtg cgactcgggc
    18901 tgggtgcggt tcttttccaa gtcgcccgtg gtgcatccgg acgacttgcg caagctcaag
    18961 gttttttcgt gggccggcaa cgccactcaa tatgacttgt ggaaggccag cgggttccac
    19021 ccggtagccc tcgaaacctc cggcatcgcg cagggctttc tgtcgggcac catcagcgcg
    19081 gtgcccacgg tgccgatctt cgccctggcg gcgcaactgg acacccaggc ccggcacatg
    19141 ctggaaatca actggggtcc gctggtgggg gcggcggtgg tgacccagcg gagctgggag
    19201 cgcgtgcccg aggccgcccg cgaggccatg ctcaagatcg ccgatgaggc cgggcgccag
    19261 atccgggccg ccggccgcgc ggagaacgac gcggcggtgg cggcgatggt gaagcgcggc
    19321 ctggttgtgc atcccatgac gcccgcggcc gaggccgagt ggctggccgt ggttgacggg
    19381 gtgaaggacc agattcgcgg caaggtggtt ccggcggacg tgtttgacga ggcgcagcgg
    19441 ctgctgcggg agcaccgtgc cgggggcgcc gggaaatgaa aggggcgggt tcaggaggcg
    19501 gcgggggccg ctcacttgcc ggccgggaaa agggcggcgc tccgggcctg gccggcgtgg
    19561 cgccgttgcc ggcggagccg cgatgagtct tgaatctcca gcaagcgccg aggcgcatcc
    19621 gggcggcggc ctgacgccgc gcacgggttt ccggcgggtg gcgagccagg cggaggattt
    19681 gctgctggtg gtggcgctgg cggcgatgct gttgctgccg gtggtggaga ttctcctgcg
    19741 ccatgtcttc cacaccggca tttcgggttc gagcgccatc gtgcagcacc tgacgttgat
    19801 tgtggggatg ctcggcgggg cggtggcggc gcgggagcgg cggttgctgg ccttgtcgcc
    19861 catccagacg ctgctccagg gccgggcgcg ggcggcggcg caggttttca gcagcggttt
    19921 tgcggcggcg gtttgcctgt tcctgtgcgt tgccagcctc gagtatgtgc tggcggtcaa
    19981 gccgctgggg aagattctgg tttatggcct gccggtgtgg gtcgtgcagc tcattctgcc
    20041 ggtgggcttt gggctggtca cgctgcggct catctggcat gcgaccggca cctggcgggg
    20101 ccgcggcctg gcgctggcgc tggcggcggc gctggcgggg ctggcggcgt gggggccggt
    20161 gccgccggcg cgcctggaga ttccggggct ggtggcgctg gcggtggcga cgctgctggg
    20221 ggcgccggtg ttcaccgcgc tgggcggcgc ggcgctgctg ctgttttggg gcaacgatct
    20281 gccgatccag gcggtgccgc tcaagcatta cagcctcact accaatgacc tgctgcccag
    20341 catcccgatc tttacgctgg ccggttattt cctcgccgag agcggcgcct cccggcggct
    20401 ggtgcgggtg tttcaggcgc tggtcggcca gttccgcggc ggcccggcca ttgtcaccgc
    20461 gctggtgtgc gcgtttttca cctcgttcac cggcgcgtcg ggggtgacga ttctggcgct
    20521 gggcggcgtg ttgatgccgg tgctgctggc cgcgcgctat tccgagcgcc acgccctggg
    20581 gctgctgacg ggggcggggt cgctgggcat gttgtttccg ccctgcctgc ccctgatttt
    20641 gtatgccatc gttgccaaca ccagcgcgca ggcgaacttg agcctcaagg aaatctttct
    20701 gggcggcatt ggccccggct tgctgctggt ggccatcacc gcctggtggg gcatccgcaa
    20761 gggttcccgg gaagcggccg accggcccgc cttccggtgg gccgaggccc gcgcggcgct
    20821 ctgggacgcc aagtgggagc tgagcattcc ggtggtggcc gtcggctcgc tgttcaccgt
    20881 gccgaccatc gtggaggccg cggccgtgac cgccaccacg gcgctggtga ttgccatggt
    20941 catccaccgc gaggtgcatc cgattcggga tttgccgcgc gtgatcaccc aatgcggcct
    21001 gctagtcggc ggcgtgctgc tgatcctggg cgtcgcgctg gggttcaccc actacctcgt
    21061 ggatgcgcaa ctgccggata aactggtcgc ctgggcgacc acggcggtga agtcgaagtg
    21121 gctgtttttg ctcgggctga acctggtgct gctgttcgtc ggcgggctgg tggagattta
    21181 cgcggcgatt attgtggtgg tcccgctgct ggtgccgctg ggggtggcct tcggcataga
    21241 cccgattcac ctggggatta tcttcctggc caacatggaa ctggggttcc tctgtccgcc
    21301 cgtcggcctg aacctgttgc tttcgtccta ccgtttcaac cggccgatgg ccgaggtgat
    21361 tcgcgccgcc ttcccgatgc tgctggtgtt gcttctcggc gtgctgctca tcacttactt
    21421 cccgccgctg acgaccttcc tgccgcggct gttccggtga ccgccgggcg gccgggcccg
    21481 gccggcgccg gcgggtattt gcggttttgg ccgccgccgc gcctgtgcta ccttgttctc
    21541 cgtgagccgg ttgaagctgt tggtttgcct gggggccgcg tccatagggg cggcggccgc
    21601 cgccgggggc tgctcgcgcg ctgccgcgcc ggccgcagcc tccccgcccg gcgctgcttc
    21661 gccgtccgcc accaatactg tcatgcaaaa cttccagaaa ccgtcccgcg ccgaactgcg
    21721 gcagaaactg acgccgcggc aattcgacgt cacccagcag gcggcgaccg agccgccctt
    21781 tcgcaatgag tattgggatc accgggcgcc gggtctttac gtggacgtgg tttccggcga
    21841 gccgctgttc agctcgctgg acaagtttga ttcgggctgc ggttggccca gcttcacgcg
    21901 cccggtggcg gccgcgagcg tgcgcgagcg cccggacgat tcgcatggca tgcggcgcac
    21961 cgaaatccgc tccgcggcag ccgattcgca cctgggccat gttttcgacg acggccccgc
    22021 ccccgccggc cggcgctatt gcatcaattc cgccgcgctg cgcttcgtcc ccgtggcgca
    22081 aatggaagcg gcgggctacg gcgcgtacct ggagccgttt atccgggccg ggcttcaccc
    22141 gcccgcgccc gcgcggcgcg atccgccggc ggcgcgttaa caggattgcc accggaaggc
    22201 cgttgcccca tgctgcccgt tgaaccgata aaaccgtgca agccttgacc cgttaatgcc
    22261 atgcttattg ttcacgttca tgttcatgta aagccggagt gcgtggaggc cttcaagcag
    22321 gcgagcctcg ccaatgcgcg ggagagcctg aaggaggccg gcatcgcgcg ctttgattgt
    22381 gtgcagcagc aggatgaccc gacgcgcttc gtgctggtgg aagcctatcg caccccgcag
    22441 gcgcccgccg cccacaagga aacccgccac taccaggcct ggcgggatgc cgtcgcgccc
    22501 atgatggccg agccgcgctc gagtgtgaaa tatacgaacc tgttcccgga agacgcggcc
    22561 tggtgacggg cgggcggggc ggtttttccg cgcatgcgct ttgaatttgc cagcgccacg
    22621 cgcatcctct tcggggccgg caccctgcgc gaggttggag cgttggcgcg ggagctgggg
    22681 cagcgcgccc tggtggtgac gggcgccagc ttgatccggg cggagcggct gcttcacctg
    22741 ttgcgcgggc acggtttgat cagcacgata ttctcggtga cccgcgagcc ggagatgggg
    22801 gtcatcacgc agggcgtggc gctcgcgcgg cagcagggct gcgagatggt catcgcgttc
    22861 gggggcggca gcgtgctgga tgcgggcaaa gccatcgccg ccatgctgac caacccgggc
    22921 gagttgctcg attacctgga agtcattggc cgcggccagg cgctgaccct gcccgcggcg
    22981 ccgtttgtgg ccatcccgac caccgcgggc accggttcag aagtcacccg caacgccgtg
    23041 ctggcctcgc cggcgcatgg ggtgaaggtc agcctgcgca gcccgctgat gctgccgcga
    23101 atcgcccttg tggatccgga actgacccgt gacgcgccgc ccgccgtcac cgccagcacc
    23161 ggcctggacg ctctcacgca gcttatcgag ccttatgttt gcacccgcgc ccagccaatg
    23221 acggatgcgc tgtgcgagga ggggattcgc cgcgccgccc gctctttgcg aaccgccttc
    23281 gcgcgcgggc atgacttggc cgcgcgaacg gatatggccc tggccagcct gttcggcggg
    23341 ctggccctgg cgaatgccgg gctgggcgcg gcgcacgggt tcgccgggcc gatcggcggc
    23401 cagtttcccg cgccgcatgg cgcgctttgc gccgccctgc tgccccacgt catggccgcc
    23461 aacctggacg cgctccgcca gcgccagccc gaggccgccg cgttgcgccg ctatgatgag
    23521 gtcgcgcgtc tgctgaccgg ccaggcggcg gcgacggccg aaaccggcat cgcgtgggtc
    23581 cgcgaactgg ttgccgattt gcgaatcccg ggcctgcgcc aatatggcct gaagccggaa
    23641 cacatcgccg acctcgtccg gaaagcctcc caggccagca gcatgaaggc gaacccgatt
    23701 gcgctgacgc acgaggaatt ggcccacata ctggaacagg cgctctgatt ccagccccgg
    23761 cccgccgcgg caggcttaac gccgtttgga ggacccgtac ccggggagag gatgcgcggg
    23821 ccttgatttt tcagcatttt cactttccgc accgcgctca aaactatccg ctgtaaactt
    23881 gctcttctgc cagggggaaa atgctggctt tctttacacc acccttaatt agagctaagt
    23941 ggtgttgaat cagtgagata aggaggcaat aaaccagggc tgcgcaatag ttgtcggcat
    24001 ctttaacact tatgcgatgt caggagaagt acggattcaa aaattgataa gttgtaagtt
    24061 gttgtaaagt ataaagatat aaatcaatac aagttattca atgtgtttgg catgagtagt
    24121 gctaggccta tttgtatagt tagtaacaat aaaaatagta agatatgaaa aagagcatca
    24181 tcattgcaac ggttttgggc gtagctaata tggccctcgc gataacttcc atcacgacga
    24241 cttacggccc gattgctggt cccaaagggg tcaattggga cgggtattat gcctatacct
    24301 gggcggtagg agctccggct ttggctgcgg atgaggtcgt tattgcggcg acgctatcct
    24361 tcaacaagat taatctgacc tcgggttctg gcaagctgtg ggttgacttt ggcacggcta
    24421 ccgcaagcaa gtcgtacgtg gactttgata ttgcgggtga ttatttcgat gggcgtgccg
    24481 ggtttaatgc cgtggatgtc ggcgtgcaga cgtggaccaa agccggcagc aagacctgga
    24541 gttgggatat tacggaactg gcggacctga acgtccgcct gcatgcgggt gacctgggat
    24601 tcctgtttga tccggactgc accttcaagg ttggcagcat cagctttaca tacaccaccg
    24661 agaaacgcgg cggtggcggt ggcggcgggg tgcctgatgg tggaatgacc gcgatgctcc
    24721 tgggcgcgac cctgatgggg ttgggctggg cgaagcggaa aatcagtgcc tgatcgctag
    24781 ctcaggctgc atttcccaat cgaactggcg ggctttggcc cgccagtttt tttgtacgag
    24841 aatcgggttt ggcaagggcg ggaaccgtgg ggacagattt gctttcacct gcccggcgaa
    24901 cgatgccggc cagagggcaa gcggtagtgg cccgggagaa tcccgtagat acaggtcgct
    24961 gctgccgcat gtggaaatca gagccgacac cgccctccgg ccgcctgctt tgcctgaaag
    25021 cacggccatc tgccgactac ggccgcagcc atcgccgcag gcttaatcgt cgtcgtcggg
    25081 cgcgggggga accagtttaa gcccgaattg tttcagtccc ctgccgtggc cgccgacgaa
    25141 ccgggcgggg tagggttggt cgcccttaat cgtccaccag atgtagcggt tgaagaggtc
    25201 atcattaatc cggtcgggct tgctgaaatc catttgggcg agttgtttgg cccagtattg
    25261 ctgtttgcgc gaggggcggg ccgtagcggc cacgccttcg tccagcggga cgttgttggg
    25321 ccgggcggtg tagggcgtga agttgggcac gttcgtgaag cattcgaaca tcaacggcgc
    25381 catggcgtcg tgctggttca tgggcggcaa gcctaaaatg cgctccatgg tatgcagcac
    25441 gcccgcctgg ttgtagaacg tgctgattac ctcgccccgc ctggtgtagg ggctgattac
    25501 gaggcaaatc gagcgatgcg cgtccacatg gtcatagccg ctttgagggt catcctcgat
    25561 gacgaagatg acggtattgc tgccgaagac gctgcgggtg atggcttcca ccacgcggcc
    25621 cagggaaacg tcattatcgg ccacctgcgc ccgtggcgag ggcggcccgc cggtatgatc
    25681 attgggcagg taaagaaaat gaaagtcctc ccaaacgcca gtagcctcgg cttggcgcag
    25741 ctctttgatg aacccgtccg cgcgcaccac gtcgggaatc ccgaggttcc acccggggac
    25801 gttggtggag ctgtagggcc gcagcgaggc gaggccgatt ttctgcacgt agcggatggc
    25861 gcccgtgccg tccagccaat cctggtagat ttccgcccag gacttggggg gtgaagtcga
    25921 ggcgtaatcc atctcgccat agttgcgaaa ggccaggccg tgttgcagga cgttgttcca
    25981 gatgaacccg gtggaggaat aggtcagcgg atcatcgccg aaggtatagc tgcgggtgaa
    26041 gccgccaaag gatttctcca gatgatcggt cacgttggct tcggtgctcc aggaatggcc
    26101 gtccgccgag accacgccgt tgcagtaaaa gttatcgagc aggacgtatt cttccgccag
    26161 ggcatggtgg ttgggcgaga cccagcgcgg atagatgcag aggttggaag cgccgttgcc
    26221 ctggggcagg tctccgaaca tctggtcgta ggtcttgttt tctttgagga tatagacgac
    26281 gtgttgaaat acggaaggct cgcccgtgcg cgcgggcacc ggcaccggcg gctggccggg
    26341 gcggagaacc cgctgggttt gccggatttg cggcacccgg ccgtcctcga acacttgcgc
    26401 ggtgtatttg gcgagcgcct cggcatcggg tatggcgatc cggttggccg tgcccaggtg
    26461 ggcggaaatc tgccaggccg tgacggcggg ttgccccagc cggctgccca ggcccttcac
    26521 gttggcgaca tagacgaaat cgcggtccgc cgcgactgcg ccgggatacc aatcggtggg
    26581 caggaacccc cgcaagatgc tatgggcatg ccgggcgttg ggcagttcca cgaccgccac
    26641 cgcgttgttg cccccgctgg ccacgaacag cgtctggcca tcccggctca aggccagccc
    26701 ggtgctggcg ctgccataag gaaaagacgg gtcgggccgc accagaatgg tctccttgac
    26761 gctccgggtc cgcgtgtcca ggaccgtgat ggtgtccgag ttggcgttgg ccacataaag
    26821 cgtcgcgccg tcggcgctga gttcaaggtc gcaggggtgc aagtccgtgc ttacctgggc
    26881 ggtttcctgg ccgcgggaaa ggttgaccac ggacaccgca ccgctggcgg cgacgccgcg
    26941 gctgtccaca atcaccggcg tgccggcgga gggggccgtc aggtcgccgg tctgggggaa
    27001 gcgcccgccc cagtccgaaa cgtaagcggt gttgccatcc ggcgacagca cgacatccca
    27061 gggcgcaatt ccgacgttga tctgccgcac aacgctgccg ctggccaggt tcaccacggc
    27121 cagcgtattg gagatggaga ggcatacata agccgtgctg ccgtcgccgg caatcgccag
    27181 cccgcacgga taggaaccgc ccgggagcgc gatggtgcgg acgaaggaaa ccaggttatt
    27241 gctggcgacc gcccactcat acagctcgtt gccggcgccg gtcgcgtaaa tatgcgagcc
    27301 gtcggggctc accgccactc cgtgcatcga ggcgccgccg cccgggaagg ccagcgtctg
    27361 ccgaatccac cacccggcgg catccaccac cagcaagttg cgcatgttct tgatatagac
    27421 cgtctggccg tccggcgaca aaacgaggtc caccggccgc ccggcaaact gaacttgccg
    27481 ccccgccgcc cgcaggatct gccgggaagg cagggactgc tgctcctccg gcaactggcc
    27541 gacctggatc tccgccggcg ccggctgcag gcggaagcgg gtttgcgccg cggtgatggg
    27601 gatgtggatc tgctgattcg tcgttgcggg gagaaagttc tgccaatcgg gggcggccgg
    27661 gcggatggct tgctggagtt gcacgtcttt ccccaggccg ctccaggcca gttcgagggt
    27721 cgaggcattg tgacccagcc ctttgatttt gagcacgcta ttgccgggcg gaagcccctc
    27781 ggcggtcggc gcgcccaggg ccgcaatatc agccggggac atccagccgt tgacaaactg
    27841 gaggctgcgc acaaagccgg cttgcgcgag tccgggccgg cccgccgtga agagctggac
    27901 cgccggcccc agcgcaaaac gcccgtccac cccgccgctc agcgattgcg cgccgacgag
    27961 gaccccgttg atgtatttca tgagctgccg gcccgcgggc gcggcgagat ttaccgcaaa
    28021 cgcaaggcga taccacgtcc ccgcggccag cgcgccatgg aactgcccct cggcgccaat
    28081 gccattgggg ctgggggccg ccgcggcgtc gctcacataa aactccgcct cgttgccctc
    28141 ccgattgaac ggatcggtct gccacagggc gcgccactgt ccactgctgg cggccgggaa
    28201 cataacgtcc ataatcaacg tgtactggtt gacgaaatgc ccgccgccat tgggccgccc
    28261 gccgtggcgc aggtagaatc cctggccggc gccattggcg ccaaaggcca tcacccgggc
    28321 gactcgtccc tggatgttct tcagcgggaa agaaacctgc tgggcggtat tgccggcata
    28381 ttccagatcg gcgcccacgg tcgcgcgcaa atcgccctgc tggaaatccc attgtcccgt
    28441 caccttcaac tcatagaggg tgaggctggc ctcgcggctg aaaaccgcgc ccgtgccgct
    28501 gctgacccgc acggagtacg ccccttgccg gtccacggtg gccgccggaa ttaccagcgt
    28561 ggcgttcgtg gcccccggca ggttgacgcc gttccggcgc cattggtagg ggagcgggcc
    28621 gccgccgcgg gcgtccacac taaaaaccgc gtccaacccg ggggtcacgg ccaggctggc
    28681 cggctgctgg ataatcaccg gtggcacgcc gggcaccgcc gccgtcagcg gcaaatgccg
    28741 cagccccgcc gcgtaaatgc ccgagacttc ccgcggcccg agcacccgcg tccagatgcc
    28801 gaggtcgtcc acgtcggcac tgccggaata ggcgccgctg ccgctgctgc cgacaagcgt
    28861 ggccagcccg gcattcagag acgccgcgcc ggaagagccg aggttgctgg agctgacggg
    28921 cacgccgtcc caatagccga tgacctcgtt ggccgggcgg ttgaaggtca ccgcgagcaa
    28981 atgccaatgg ccatcggaaa aagccgggct gagcgccaga tcgcgccgcg ggccgcccgc
    29041 ggcattccag ttgatgtttt tggaggccat gctggaaatc acccagccga cgttggcgcc
    29101 tccgctccaa ttcttattgc cgataagggc gccgtcgctg ttcgtggtga cgcgcaccca
    29161 gagcgatacg ctgaagctgt tggagtaaat ccattcaaga ttgcccagcg acaccgccca
    29221 atcgccgggc tggccggaga ccgaggcatt ggtgaaggat gccgccgcgc caatcatgcc
    29281 gggcgcataa cgcggaccgg gtcccgctcc cccggggtag agcgttccgt gattggtcgt
    29341 gccggcctgc gcgttgaggt ggccatcgaa gctcaaatac accgccaggt tgttggtgac
    29401 cgaagccgcc tggccgggcg aaagcaccgt cagctcgggg gtgtcgctgg tggcgaggac
    29461 gctctgcttc cccacggcaa cggtggaatt gcccgtgttg ttcgtgccgg gggccgccca
    29521 gacggaggct gccgccgcca atccggctgc cgctagaaaa gccgcccgcc atcgcatcgt
    29581 ggacctatgt tttagccgtc gcatagatgc tggtgatgat gattctctaa cattcgccag
    29641 cttgagacaa ggctcggttg atccggaacg gctcaatcag gtaaaattca actgcccgcc
    29701 ctcgttccat cggagcctgg gcaggcctaa ccgcccaaac ccaagtccca acaaaccttt
    29761 tgtttagcga attcaaggat atggctgctg aattaagaac ctttcgcttg taaaatcaat
    29821 agtttatgta tcgagaggga taaaacagag gcagaatcat gccgacttcg gcataccctg
    29881 aacttaccct gcctataggc cgtgtacaag gggtgtacaa gggatgctca tcgggtgtac
    29941 cagctaaaat cttatgaact cccg
//
LOCUS       DAOPQG010000037        29472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2027_length_29472_cov_15.931944, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000037 DAOPQG010000000
VERSION     DAOPQG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 29472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29472
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2027_length_29472_cov_15.931944"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKI07_05970"
     CDS             complement(<1..439)
                     /locus_tag="PKI07_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50635.1"
                     /translation="MTPKTVLASEHALARRCPACPPRKRNAKAPIPVQGIIALALVLA
                     DGGRAAVPESYRTSWSHPEVNARIERGIEQFRKGDCLLEVVDAAGKPVPDARVEARQT
                     RHEFLFGCNAFVLGQLKPEDLEQRYEAAFARLFNFATVPFYWEG"
     gene            complement(1513..2268)
                     /locus_tag="PKI07_05975"
     CDS             complement(1513..2268)
                     /locus_tag="PKI07_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOC50636.1"
                     /translation="MPRQRFKCAQTNMANSIAELKQAAIVRTLRGCQLFAGLETADLK
                     SIAAITRVKPLNKGGCLFREGAPAQGFYIVQKGAINVHRVNAAGKEQVIRVFRDGESF
                     AEAALATASGYPADARALESSQVLLVEKTGFLSLLKQQPELGLRMLGSMSQHLRSLIA
                     QLEDLTLKDVETRLANWLIKRCPDPESDRPVAVELKTTKRMLAAELGTVSETLSRTLA
                     KLRQQNLVAVTGRNLTVLSPLKLRQLLRRNLGE"
     gene            2559..3365
                     /locus_tag="PKI07_05980"
     CDS             2559..3365
                     /locus_tag="PKI07_05980"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC50637.1"
                     /translation="MDNRFVYAVVSARAGGLSVGINLTPDKSCNFRCVYCEVHRGEER
                     PAELVEVEAMAGELKKTLALVRSGRLRERPPYRALPDDLLQLRHVALSGDGEPTLSPR
                     FAEALQAVVHVRAMSGAPFFKLVLLTNATGLDLPHVQRGLKHLTSSDEVWAKLDGGTQ
                     DYINKVNRPAVPLEKILSNILLLARQRPVVIQSLFPAIHEEEPPSEEIEQYAQRLIEL
                     RRAGAQISLVQIYSATRPSPNSESGHLPLRVLSRIAQTVRQTTGLAAEVF"
     gene            complement(3369..3821)
                     /locus_tag="PKI07_05985"
     CDS             complement(3369..3821)
                     /locus_tag="PKI07_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC50638.1"
                     /translation="MQKRSPVLVAEDDPNDVMLLCRAFRENKVLNTLQIVHNGEEAIN
                     YLAGQGQFADRAAYPFPVLFLLDLKMPVKDGLEVLRWLHEHPEIPTKLPVVVLSSTEL
                     PNETQLAYALDIQACIVKPISFADLREKVRILKEYWLDYEADFTAPVA"
     gene            4133..5254
                     /gene="mtnA"
                     /locus_tag="PKI07_05990"
     CDS             4133..5254
                     /gene="mtnA"
                     /locus_tag="PKI07_05990"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOC50639.1"
                     /translation="MNVTVHGRTRHFRTVTFDAGRNAVRLIEQRLLPHEFRIVATRDF
                     RQTARAITDMVVRGAGAIGATAAYGLAQGAHAFRGRRLADFQRHMGVVSRALKAARPT
                     AVDPANAIHYVSQFMRRGRTIAERQALALAAAEEFASADVRHCEAIGRHGARLIRSGM
                     NVLTHCNAGWLAFVDIGSATAPLYAAQAQGRKFHVYCDETRPRSQGATLTAWELAQQG
                     ISHQVIADNAAGHLMQRGKIDLVIVGSDRTLGRTGEVANKIGTYTKAVLAGRHGIPFY
                     VAIPLSTIDWELKSGRDIPIEERHEEEVLGAWGVPAGAGRARAGRRAYVRVANPTSGA
                     LNPGFDVTPAELIAGIITPVGILKPSELWARRRELGWAG"
     gene            complement(5262..5729)
                     /locus_tag="PKI07_05995"
     CDS             complement(5262..5729)
                     /locus_tag="PKI07_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50640.1"
                     /translation="MSYDRADFDYSTDADPLPKGHAGTHIGMFLAWAVQHGLVSDFHR
                     QHSAALLARLQRREITGRQFFEAACPGRFSETDLNDEGNQFVQYYYVDEAGRRGEYFA
                     DYKTTLAARLPSFWHVADTWENYEKIAAVITRRYEQWKNPRARKRWWQFWKSR"
     gene            complement(5784..7244)
                     /gene="purF"
                     /locus_tag="PKI07_06000"
     CDS             complement(5784..7244)
                     /gene="purF"
                     /locus_tag="PKI07_06000"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOC50641.1"
                     /translation="MQTYPKHYCGVFGVYGHPKAAELTYYGLYALQHRGQESAGIVTS
                     DGRQFYTHKGMGLVPQVFRGNVLQEMPGLMAIGHTRYSTTGSSHLRNAQPLTVDCARG
                     QIAIAHNGNLTNAARLRAELEARGSIFQTTVDSEIILHLLAQPTQQGEPNNLVRSLHR
                     IEGAYSLVIMTESELIGVRDPHGFRPLCLGRIDGAWVLASETCALDLIHAQYVRDVAP
                     GEIVAINRDGLTSWQAFPDHTQRAFCIFEYVYFARPDSAIAGRSVYQVRVEMGRQLAR
                     EFPIAADIVVPVPDSGNCAALGYSLESGIPFEMAFVRNHYVGRSFLQPSQLIRDFDVR
                     VKLNLIEELVKGKRVIVVDDSIVRGTTCRTRVNNLKEAGAREVHVLVSCPPHRHPCVY
                     GIDFPDRNKLMAANYSLEEIRSYLNADSLNYLSQAGLLQATGLPANAFCMACYDGHYP
                     VHFDPAVDKHIIERRNGHRPGLAEGLAREKAQPRLL"
     gene            complement(7263..9617)
                     /gene="purL"
                     /locus_tag="PKI07_06005"
     CDS             complement(7263..9617)
                     /gene="purL"
                     /locus_tag="PKI07_06005"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOC50642.1"
                     /translation="MTDPAITPELVQKHNLTPEEYEKLKAVLGRVPTYTELGIFSVMW
                     SEHCSYKNTRPLLKTFPTQSPRILVGAGEENAGILDIGDGLAIAFKIESHNHPSAVEP
                     FQGAATGVGGIVRDIFTMGARPVCAANSLRFGPITAAGEPGPAVANNRRLFGGVVAGI
                     AHYGNCFGIPTVAGEIYFDPSYEGNPLVNAFCLGVLRHGQIVRGHARGVGNPVFYVGP
                     ATGRDGLAGAAFASQDLTAESAEQQRGAVQVGDPFMEKLVCEACLELLATGAVAGIQD
                     MGAAGLTCSTCETAARAGTGIEIELDKVPQRAPGMTSYEIMLSESQERMLIIVHRGRE
                     AEVKRIFDKWDLPWAEIGVVTDTGRMVVRHHGQVVADIPARKIADESPVYERVAREPE
                     YLQAVRAFCLDGVPDTTNPAAHLLALLAWPTIASKNWVYRQYDHMVRNNSVVGPGSDA
                     AVLRIKEDSVPAPPNAASALRPVPEKFIALSVDGNGAYVYLDPYEGSKIVVAEAARNL
                     ACTGAMPLGATDNLNFGNPHNPELFWQLKESVRGLADACRAFNAPVTGGNCSLYNQHP
                     NGPIDPTPTVAMVGLIENPEHITTQWFKDEGDVILLLGELVDTSDPLLGLGGSAYLQL
                     IHGMKNGTPPRCDLAREKELHLALRALIHSGVVKSAHDCSEGGLAVALAEGCISQSVA
                     RATPRLIGAQVDLTPLTEAPGRDGIRLDALLFGETQGRILISVDAVNAAKVLAQARIL
                     GVPTARLGTVGGDALRLQVPGAELNCPVRDLHDRWWNAIAEAMH"
     gene            complement(9671..10381)
                     /gene="purQ"
                     /locus_tag="PKI07_06010"
     CDS             complement(9671..10381)
                     /gene="purQ"
                     /locus_tag="PKI07_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOC50643.1"
                     /translation="MNFAVLQFPGSNCDQDVVHALRHVLGHSVELRWHKEASLGAADA
                     VVVPGGFSYGDYLRTGAIARFSPVMEAVRQFAAAGGLVLGICNGFQILCEAGLLPGAL
                     IRNRSLQFRCEHIFLKTSTPDSPFTNRIPPDNLLRLPIAHGEGCYYADADTLARLKAN
                     HQILWRYVNAQGEITADANPNGALDHIAGICNEGRNVAGLMPHPERASEPILGSTDGR
                     LILESMIAALASRPTAAG"
     gene            complement(10396..10635)
                     /gene="purS"
                     /locus_tag="PKI07_06015"
     CDS             complement(10396..10635)
                     /gene="purS"
                     /locus_tag="PKI07_06015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOC50644.1"
                     /translation="MKAKIVVMPKQAVLDPQGKAIQNALTQMGYHGIGAVRVGKYLEI
                     ELTNSDPEAARQQIDAACHKILSNPVIEDYRFEIE"
     gene            10827..11420
                     /gene="pgsA"
                     /locus_tag="PKI07_06020"
     CDS             10827..11420
                     /gene="pgsA"
                     /locus_tag="PKI07_06020"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOC50645.1"
                     /translation="MNLPNQLTISRFLLTIAFLTVMFSAIQFHRTIALLLFIAAALSD
                     YLDGVIARRRKLVTNFGILMDPLADKIMVCSAFIAFVGLGWMPAWMVVIIVARELAIT
                     GMRLLAASRNVVLAAEGCGKYKTVAQIVAIISIFVVASYPEWGAVGRVVFGYPWPGPT
                     PWAQWFTELAKWVAVGLTFLSGWLYLWRNRAIYLQDL"
     gene            11417..11923
                     /locus_tag="PKI07_06025"
     CDS             11417..11923
                     /locus_tag="PKI07_06025"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOC50646.1"
                     /translation="MKLWIAQGFGVGRIPLAPGTFGSLLGLLWFGLLLLTGHLWLFLA
                     GTLAALALSVWLCGAAERSLGQTDPGSVVLDEIAAMPLCFLGWMAVQFWHTGAWPALS
                     AFFSARTWPLTLAVFAAFRLFDILKPWPVRQSQSLPGGWGVTMDDVLAAVYVNVIVLL
                     AIALAARF"
     gene            complement(11958..12461)
                     /locus_tag="PKI07_06030"
     CDS             complement(11958..12461)
                     /locus_tag="PKI07_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC50647.1"
                     /translation="MSTVAEQLRQGREARSLTVQQVAEITKVRSDHIRAVEEGNYEVF
                     SATVYVRGFVRSYASLLKLDVPQIMVALDQELERSAKFAEPPPLAKRPRNLADFLMLQ
                     LSQVAWRQWLIALGAVALFIAALWGWVAWRHHDPLKKVSPGRYPSAPDNWGETLPLPP
                     APRSPAR"
     gene            complement(12558..13505)
                     /locus_tag="PKI07_06035"
     CDS             complement(12558..13505)
                     /locus_tag="PKI07_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOC50648.1"
                     /translation="MHPPASSFLTRRRFLARTAMVAGACALPGMPGQPTVCLGNIRPF
                     GRKLRDRLWMWGHDAGSLKNSFGIGSQGGDILPGAAIKYMGIPNVCMVRFTGTPRPPF
                     EDYARQFAHARRLTWSFVDGDSGHTTEQKKQLALGLAAKLPNLVGLDMDDFFLGNAVP
                     KTEGGEAPAHLSVEQVRQIQQELANRKRRLELSLVLYSHQLHPAIQRHLENVDVVYFW
                     TWQARDLSALERNFAAYRKINPTGRTRLGIYMWDFGAGQPISTEAMEHQCRLGWQWLS
                     QGQIEGLIFHCTPLCDMNLAAVEWSRKWIARHASDRVGG"
     gene            complement(13868..14704)
                     /gene="thiM"
                     /locus_tag="PKI07_06040"
     CDS             complement(13868..14704)
                     /gene="thiM"
                     /locus_tag="PKI07_06040"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOC50649.1"
                     /translation="MTASPNTFVLLEKIRQQSPVVHHLTNWVTIYDCAQVLKTLGASP
                     VMAHAREEAAEMAQIASAVVLNIGTLTTDLVEAMKLAARSANQKGIPVVLDACGAGAT
                     ALRDRKSLELLNEARVTVIKGNASEIARISGADVKTRGVDATEISADLVSLAQRLADQ
                     RQATVVITGQWDIATNGKSTYLVKNGHSLMTRVVGTGCMAASIIGAFAAVEHDATLAA
                     ACALACFGIAAEIAAEKATGPASFKERLFDCLYRLDWPTVDHRQRIDILQQPPTARQD
                     KP"
     gene            complement(14744..15397)
                     /locus_tag="PKI07_06045"
     CDS             complement(14744..15397)
                     /locus_tag="PKI07_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOC50650.1"
                     /translation="MRRLTELTAALLSCGMIQPGYREAIADYIRAQAKPPDKFSHQPR
                     LYRLARRLAEDKPYDDDILYAAAWMHDLGVFVGHRPEEPAALAAWDHVAYAAREVPAI
                     LRQFGFPEDKIPATLEAVRTHLPTCQPTSFEGTLLRDADLLEQLGAVAVLRVVSKVGR
                     DTRFVRHADAVQALRCNLERLPTLLELPSAHRLAEPRVKALKAFLEAVESEVDGGDL"
     gene            15809..16114
                     /locus_tag="PKI07_06050"
     CDS             15809..16114
                     /locus_tag="PKI07_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOC50651.1"
                     /translation="MSAKLFVGNLSFNITENDLQDAFAVHGTVVEANLMMDRATGRPR
                     GFGFVTMSTPEEAEKAIQALNNTELAGRKLTVNIARPREERPIGGSRGYGGRGRERY"
     gene            complement(16287..17138)
                     /locus_tag="PKI07_06055"
     CDS             complement(16287..17138)
                     /locus_tag="PKI07_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC50652.1"
                     /translation="MSQNTLTLGHSPDPDDAFMFYGLARDLIPTGGFRFQHILQDIQT
                     LNERATRGELDISAISIHAYAYVSGHYALLPSGASMGDGYGPMLVARDKLSAAEIRRK
                     RIAVPGTMTSAFLALQLWLNQPACELDCLVVPFDQIFAAVRDGRAEVGLIIHEGQLTY
                     QQQGLQMCEDLGAWWGRTNEGLPLPLGGNVIHKRFPPAIRRTVSDVLTASIQYSLDHR
                     PAAVQHALQYARDMGLDLADKFVGMYVNHWTLDYGEKGRESIRRFLGQAAARGLIPRQ
                     PELEFVA"
     gene            17265..19418
                     /locus_tag="PKI07_06060"
     CDS             17265..19418
                     /locus_tag="PKI07_06060"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOC50653.1"
                     /translation="MGHLDTLNPQQRLAVETIHGPVLILAGAGTGKTRVITHRVAHLI
                     ERGIVPGHILGVTFTNKAAREMQERVAQLIRKRLRTPGGEAPGGSPASAPAAAPLSPT
                     ICTFHSLCVRILRQHIEKLGYKRNFVIYDEAEQLGVLKKILAQISTPDAKADPAAILA
                     WLSRLRNGGEHATALSDPGWRALAEHLRARYESALRACNAVDFDDLILLTLRLFREQP
                     VALEACRARYRYVMVDEYQDTNAAQFRLVHDLTREHRNLCVVGDDDQSIYGWRGAEIA
                     NLLEIEKHYPETRVIKLEQNYRSTNTILAAANAVIRHNVRRRGKRLWSQKGDGPKITL
                     HAFASDEEEARTIAEQIEFARLAHRTPWAHQAILFRTNAQSRPLETALRQAGVRYRLV
                     GGQSFFDRREIRDFLAYLKALLNPSDDVSLLRIANVPARGLSDATMERLLAASSERKC
                     PVFAAMKNPAVTTSFPSRPRESIARFVELIERTQEQLLAPREPGARDPRALQNWANGF
                     LTEIGYFAEIRRLEKNPETAENRLRNLKDLIATLDESASAAPSRFEGLQTFLEQITLD
                     AGREEEKEPDANDGVTLITMHSCKGLEFPHVYVVGMENGLLPHSRAQADGTLDEERRL
                     FYVAITRAMQTLTISHCGARKKYGTPVPCHPSPFLQDLPPELVEHADERARQPVTPQS
                     AKANFAILRASLDNAEPPPAPSRAGPNRARGETPR"
     gene            complement(19419..20366)
                     /locus_tag="PKI07_06065"
     CDS             complement(19419..20366)
                     /locus_tag="PKI07_06065"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOC50654.1"
                     /translation="MNLAVQIGGLALPNPVTVASGTFGYGVEYAQLLDLNQLGAVVVK
                     GIRRDPVRGNPTPRLVEVTSGLINAIGLQGPGVDGFIRSYWPFLQQLKVPTIINIWGT
                     SVEEYAEVARRLDALGGVGALELNVSCPNIKEGGAQFGTDPALLRQVVAACRAATRLP
                     LITKLSPNVVSIVPYAQAAEAAGSDALSIMNSYPAMAIDVETRRPVLANVTGGLSGPC
                     IKPIALKLVWEAAQAVRIPIIGMGGIQNTADALEFIMAGATAVAVGTANFYAPQTALQ
                     IIAGLRQFMRQENLADVRQLIGCARAGKGRAPEATVRHP"
     gene            complement(20363..21148)
                     /locus_tag="PKI07_06070"
     CDS             complement(20363..21148)
                     /locus_tag="PKI07_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOC50655.1"
                     /translation="MVEQTAQIIANERDTGPYFRLRVRAPLIAPRVQPGQFVHVRILP
                     LKHALLRRPFSVFQVAGDTLSLLYKTIGQGTEALARMRPGEELSLLGPLGHGFTVPRP
                     DGEIPLLVAGGYGMAALYLLAQRSPRKGLVFVGGRQRADILCEAEFRALGWEVRVTTE
                     DGSHGEKGVVTEPLRAELQRRAPGRKLFACGPTPMLRAVGRLAAEHGLPAELSLDQHM
                     GCGIGVCLACVVPIKTAGGWEYQRTCTEGPVFDFRQIAWEAAE"
     gene            complement(21226..23217)
                     /gene="shc"
                     /locus_tag="PKI07_06075"
     CDS             complement(21226..23217)
                     /gene="shc"
                     /locus_tag="PKI07_06075"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HOC50656.1"
                     /translation="MEVASLRQSRAEGNPRQTDFGFKPRVELEAAIERSQRYLLGEQK
                     AEGYWIGELLVDATLVSDMVAYHHWNGQVDPQWERKAVNHIFSKQLPDGGWNIYHGGP
                     AEVNATVKAYLALKLAGVPVTDPRMLRAREVAVNLGGVPRMNTFSKLYLALLGLFPWE
                     YVPTIPCEVILIGKWFYVNFNEMSSWSRSMFVPLAIINHFKPTRKLKSGVKLDELYPE
                     GIHGRDLALAPDPERITWRNFFLWLDRVHKFAEWFAQHGIHPFRKRALRKAEQWMLER
                     FEGSDGLGAIFPAMLNSLIALKALGYPDDHPQVVRAAAELKKLEHETEQSVRIEPCLS
                     PVWDTAIVSICLRESGVPADHPALKRAAEWLMAREIRFRGDWIYKNPAKVEPSGWVFE
                     FDNKWNPDVDDTAMVLLALRLVATDDRQKRDECFQRGLTWMMTFQCKDGGWAAFDKDC
                     TKGVLEKVPFADHNAMLDPECADITARILELLGYEGWSVEHPQVQRALRFIRERQEED
                     GSWYGRWGVNYIYGTWQVLRGLRALNLDMRQPWLLKARDWLESVQHEDGGWGERCNTY
                     DDPVFKGQGPSTASQTAWAVMGLCAFGDPERPSLQRGIEYLVRTQNADGSWTEHETTG
                     TGFPKVFYLKYDMYRNSWPLLALATYRQLLAPDSAARRA"
     gene            complement(23365..24255)
                     /locus_tag="PKI07_06080"
     CDS             complement(23365..24255)
                     /locus_tag="PKI07_06080"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOC50657.1"
                     /translation="MRLIGYLAEEKAARTFADFLYVRQIETQLEFEPAEGWGIWVADE
                     DQIQDAERLLTEFRSNPADPQYRAQAREAADRRAEAEKREAGYRKKQQERRHLFQAPT
                     AYGFGPLTFALMVISVTVFILSGMGKDFRPIQGLFIRNFVAGGSAGAGLPEVLQGQVW
                     RLVTPIFIHFNLLHILFNLLWLHDLGSMIEGRQSTGMLARLVLGLAVVSNLVQFWFGG
                     PVFGGMSGVVYGLLGYIWMRGKFDPGSGLYLHPTTLTMMIIWFFACFTPIVPHVANAA
                     HAGGLVMGIAWGYLSSLRYR"
     gene            complement(24292..25191)
                     /locus_tag="PKI07_06085"
     CDS             complement(24292..25191)
                     /locus_tag="PKI07_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC50658.1"
                     /translation="MPALSRREFLVRTLVGSAALVLAPELLAASQRRDPNVWALLSDI
                     HLSADASMVQRGINMTDHFTQATREVLALRKRPAGVIITGDCAFNSGQSVDYEHLARL
                     LQPLRQGQVPVYIAMGNHDNRERFRAVLEAGGAPPRPLADRQVALLPTPRVNWIILDS
                     LEKTLSTPGLLGPEQLDWLARTLDDHPKKPTLILAHHNVGTTAKRSDLKDSEAFLNII
                     RPRKQVKAYIYGHTHSWHVEQDPSGIHLINLPALGYVFRPEDPSGWVLATLKRRGMRL
                     ELRSMDPAHRAHGQVVELKWRAA"
     gene            25328..25852
                     /gene="lepB"
                     /locus_tag="PKI07_06090"
     CDS             25328..25852
                     /gene="lepB"
                     /locus_tag="PKI07_06090"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOC50659.1"
                     /translation="MATSNEAKTAPGWGQRALIGRHPRRTLVRIILLAITSLILFRYV
                     LLPVRVSGGSMLPTYKENGVNFVNRLAYLFHEPRRGDVVAVRLLAGAHRLYLKRIVGL
                     PGETVAFHHGRLLINGQPLAEPYVKFPCDWNQNPVPVGPDQYYLVGDNRDNPREGHEQ
                     GRAARALIIGKVLL"
     gene            25857..26363
                     /locus_tag="PKI07_06095"
     CDS             25857..26363
                     /locus_tag="PKI07_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50660.1"
                     /translation="MKEWTIRILLVAALAALGIWGWRTLHPDPEQVIRRQLQEMAKAA
                     SFTAEQGLAAQAWYASSLPEFLALDIEVTLRVPGVPATLSGRDEVMQLLTVSRRVCRS
                     LRLRLPDLKISLAPDRTMAEVYATGEADVTYASGEKASFLQELRLRLVKVKQGWIVQQ
                     VETVKTLT"
     gene            complement(26356..28554)
                     /locus_tag="PKI07_06100"
     CDS             complement(26356..28554)
                     /locus_tag="PKI07_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOC50661.1"
                     /translation="MEEKILSLLGRANSAPLNVAELRARLGFRRDRREDLARLLARME
                     RNGQIMRNRPGNRYTLPRAAELVPGRIRMNRQGTGFLEADDPRIPVIRIPGPATAPAL
                     HGDRVLVRREGAPPRARRTGAKEAAGRVVRVLERARTQLVGTLRRNQQSFYVLPDDPR
                     IGHEIHVPPPRQTGRPARVNDKVVVRLGEWTPRQAGPAGEVIEVLGPPDAAGVDMLSV
                     IRQYQLPLHFPRKVLAEAQALGREVTPADRAGRVDCRAHPVITIDPSDAKDFDDAICL
                     QRAGAGQWKLWVHIADVSHYVRPGSALDEEAARRGNSTYLVDRVVPMLPAALSEELCS
                     LKPQVERLTQCAEFLLTADGRVRATRFYSAVIRSQRRYDYGEVLALLARPPRNPLEQM
                     LHDAHALAGKIRRARFQAGALELDFPELKIRLDAHGRVLRLEREASDSAHQLIEEYML
                     LANEAAAGRLRARPGPAIYRVHEPPEEARLEEFRQEVRSQHIPCGRLSNRREAQKLLA
                     RLATLPIGPALKIGFLKSLPRARYAVEPLGHYGLAKPDYTHFTSPIRRYADLVVHRAL
                     LGPPGRPVAARPLPEMAEHLSATERNSDDAERDSREAKLFAFLAAQLQSAHREHYEAR
                     VIEVRSFGCRVDVPGLGLSGLVPAAGAGAGDGPPDAQRGRPPRRGARRGLKLGDTVEV
                     RVAKVDTGQRQVDFQLAEPARERRPKPGKPSGKAPPVSRAGGPPANRRLR"
     gene            complement(28636..29271)
                     /locus_tag="PKI07_06105"
     CDS             complement(28636..29271)
                     /locus_tag="PKI07_06105"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOC50662.1"
                     /translation="MKNGFRALPRSFYEPSARTVAPALLGHWLIRNTANGACGGAIVE
                     TEAYLVGDPACHGAAGLTARNRVMFGAPGHGYVYLIYGNYFCANVVCQPAGVAEAVLI
                     RAVEVAFGEDFMREHRSAESARELTSGPGKLCQAMAIDRGLDGVDLCDAAAALFVARN
                     PAVAEFRRKRGPMITTVRVGLKKAADAPLRFYLAGSLFVSRRHPPAAKRVG"
BASE COUNT         5144 a         9548 c         9506 g         5274 t
ORIGIN      
        1 ggccttccca gtagaacggc acggtggcaa aattgaacag gcgcgcgaag gccgcttcgt
       61 agcgctgctc caggtcctcc ggtttcaact ggcccagcac gaacgcgttg cagccaaaca
      121 ggaattcgtg gcgggtctgg cgcgcctcaa cccgcgcgtc cggcacgggc ttgccagccg
      181 catccaccac ctccagcagg caatcgcctt tgcggaattg ctcgatgccg cgctcgatgc
      241 gtgcgttaac ctcgggatgg ctccacgagg tgcgatatga ttccgggaca gccgcccggc
      301 ccccgtccgc cagcacgagc gccagtgcga ttataccctg aaccgggatg ggtgctttgg
      361 cgttcctttt ccgtggcggg caggctgggc agcggcgcgc cagcgcgtgc tccgaagcaa
      421 gtacagtctt tggcgtcatc aaagctgatt ctggagggac cgtcgagcat gtcaatctga
      481 caagcccgct ggggcagcat cgtggccgga ccgggcggtt cccgggcagg cgcgactgga
      541 ccaccacgga cggtgacaaa gccgacggcg cgcgggcgtc ctcgcccgct ttgggcctgg
      601 gaacgcccaa aaccttcctc ttcgcgtggg agtgcaaacg atttaagcgg gcggggacgc
      661 ccgcgcgccg gcacgatgga aatttggtca ctggccctgg caggaccacc ggattgcgtt
      721 ccagggcctt gcgcggacgc aagcgcggta tcctgcaggg tgaaagactg ccgggttccg
      781 aatcggagtg agccgccacg gaggagcgcg cacaagcccg ctggggcagc atcgtggccg
      841 gaccgggcgg ttcctgggca ggcgcgactg gaccaccacg gacggtgaca aagccgacgg
      901 cgcgcgggcg tcctcgcccg ctttgggcct gggaacgccc aaaaccttcc tcttcgcgtg
      961 ggagtgcaaa cgatttaagc gggcggggac gcccgcgcgc cggcacgatg gaaatttggt
     1021 cactggccct ggcaggacca ccggattgcg ttccagggcc ttgcgcggac gcaagcgcgg
     1081 tatcctgcag ggtgaaagac tgccaggttc cgaatcgggt gaaaccgcca cggaggagct
     1141 cgtccgaccc aggcgttgtg gctggcacgc tcttgttccc tccccaacat gaacacaccc
     1201 gccgccaaac gggccaggcg gaggaggcga acgctggcgc cgggcatcgt gtcggatggc
     1261 tggtgcagcc gctgtgcctt atacggtccc gcgggcgagt gctttgattt tgcagcaaaa
     1321 agtgggcgct gcggcggctg ggtttggtac gtgtggaacg gccagcagtg gcggcgacgc
     1381 tggcggaaag ggtttaatcc caagaccgcc aagcggctgg ctggaagaag acagaggacc
     1441 aacgggtgga gagtgacgtg tgggatgaaa ggctgaaaga cgggcggggt ttcagtgttg
     1501 ggaagccgag gcctattcgc ccaggttgcg gcgcagtagt tggcgcagtt tcagcgggga
     1561 caggacggtg aggttccggc cggtcacggc gaccaggttt tgctggcgga gcttggcgag
     1621 cgtgcgggag agggtttcgc tgacggtgcc caactcggcg gcgagcatgc gcttggtggt
     1681 cttcagttcc accgcgacgg gccggtccga ttcggggtcg gggcagcgtt tgatcagcca
     1741 gttggccagg cgggtttcga cgtctttgag ggtcaggtct tcgagttgcg cgatgagcga
     1801 gcgcagatgc tggctcatcg agcccagcat ccgcagcccc aattcgggct gctgcttgag
     1861 cagggacagg aaccccgtct tttccaccag caagacctgc gaggattcca gggcgcgcgc
     1921 gtccgccggg tagccggacg cggtcgcgag ggccgcctcg gcaaaggatt cgccgtcgcg
     1981 aaagacgcgg atcacctgtt ccttgccggc ggcattgacg cggtggacgt tgatggcgcc
     2041 cttctgcaca atataaaacc cttgcgccgg ggcgccttcg cgaaagaggc agccgccttt
     2101 gttcagcggc ttgacgcgcg tgatggccgc aatggatttc aagtccgccg tttccaggcc
     2161 ggcaaagagc tggcaaccgc ggagggtgcg gacaatggcg gcctgtttaa gttctgcgat
     2221 cgagttggcc atgttggtct gcgcgcattt gaaccgttgc cggggcaaaa gtaaatcccg
     2281 caaatcgcct gccgtagccg cggccgcccg tgccggggac ttcagatatc agcgaagcta
     2341 attacaggat aacaagtgga ggggctgcca atatatgtct attctttagc aagcatcgtg
     2401 tagagagccg cgggcggggc ggccaaggta gtctggttat tgtggaagaa gtcattccaa
     2461 aatccacaac tgcggttggg ccggcggaaa agaagcggaa agggccagag cggcttcccg
     2521 gcggccagga aaccgccttt ggctcgccgc gcgatttttt ggacaatcgt ttcgtgtatg
     2581 cggtggtgtc cgcgcgcgcc ggcggtttgt cggtcggcat caacctgacg cccgacaaga
     2641 gctgcaattt ccggtgcgtc tattgcgaag tgcaccgcgg agaggagcgg ccggcggaac
     2701 tggtggaggt cgaggccatg gccggggaac tgaagaagac gctggccctg gtgcgcagcg
     2761 ggcggctgcg cgagcggccc ccctaccgtg cgctgccgga cgacctgctg caactgcggc
     2821 acgtggcgtt gagcggcgac ggcgagccga cgctttcgcc ccggtttgcc gaggcgctgc
     2881 aggcggtcgt gcatgtgcgc gccatgagcg gcgccccgtt tttcaagctg gtgctgctga
     2941 ccaatgcgac cggcctggac ctgccgcacg tgcagcgggg gctcaagcat ctcaccagca
     3001 gcgacgaggt gtgggccaag ctcgatggcg gcacgcagga ctacatcaac aaggtcaacc
     3061 ggccggcggt gccgctggag aagattttgt ccaacatcct gctgctggcc cgccagcggc
     3121 cggtggttat ccaaagcctt tttcccgcca ttcacgaaga agaaccgccg tcggaggaaa
     3181 tcgagcaata tgcccagcgg ttgattgagc tgcggcgcgc cggtgcccag atttccctcg
     3241 tgcagattta ttccgccacg cgcccgtcgc ccaattcgga aagcgggcat ttgccgctcc
     3301 gggtgctctc gcgcatcgcg cagaccgtcc ggcaaaccac cggcctggcc gccgaagttt
     3361 tttgacgctc aggccaccgg ggcggtgaag tcggcctcgt agtccagcca atactccttt
     3421 aggattcgca ccttctcccg gaggtcggca aagctgatcg gcttgacgat gcacgcctgg
     3481 atatccagcg cgtaggccag ttgcgtctca ttcgggagtt cggtcgagct cagcaccacc
     3541 acgggcagct tggtcgggat ttccggatgc tcatgcagcc agcggagcac ctccaggccg
     3601 tctttaacgg gcatcttcaa atcgagcaga aacagcaccg ggaacggata cgcggcgcgg
     3661 tcggcgaact ggccctggcc cgccaggtag ttaatcgctt cctcgccgtt atgcacgatt
     3721 tgcaaggtgt tcagcacctt gttttcgcga aaggcacggc acaggagcat gacgtcgtta
     3781 gggtcatctt cggctaccaa taccggacta cgcttttgca tacaatgctt gcgcccatcc
     3841 atagcggatt acctgtgcgc tggcaagccc gttaaagttc gggcgcgagg ccgttccgga
     3901 tcacccagcg gggagcccgg cggaaagggg gatgcgtggc gcggggggcg cgggttgcgc
     3961 tgggagggga atgaagcccc gccgcgcgcg ggtttgttcg gcggcttgac ttgttcgtag
     4021 cgccttgaaa cgatttgagg gttcctatgc agcaaatccg cactactaga aaacgattgt
     4081 ccgccgggag gagaaccccg gaggccaggg gcgacgaaag gagcccgctc gtatgaacgt
     4141 cactgtccat gggcgcaccc gtcatttccg caccgtcacg ttcgatgccg ggcgcaacgc
     4201 tgttcggctg attgagcaac ggctgctccc ccacgagttt cggattgtgg ctacacgcga
     4261 tttccgccag accgcgcggg cgatcaccga tatggttgtg cgcggcgccg gcgcgattgg
     4321 agccacagcg gcgtatggcc tggcccaggg cgcgcacgct tttcgggggc ggcgcctggc
     4381 cgacttccag cgccacatgg gcgtggtttc ccgcgcgctg aaagccgcgc ggcccacggc
     4441 ggttgatccg gctaacgcga ttcactatgt cagccagttc atgcgccggg gccggacgat
     4501 cgcggaacgg caggccctgg cgctggcggc ggcggaggag tttgccagcg cggatgtgcg
     4561 gcattgtgaa gccatcggcc ggcatggcgc ccggctcata cgcagtggaa tgaacgtgct
     4621 cacccactgc aatgcgggct ggctggcctt tgtggacatt ggcagcgcca ccgcacccct
     4681 ttacgcggcg caggcgcagg ggcggaaatt ccatgtctat tgtgacgaga cgcggccgcg
     4741 ctcgcaaggc gcgaccctca cggcgtggga gttggcgcag cagggcattt cccaccaggt
     4801 catcgccgac aacgccgccg gtcacctgat gcagcggggg aaaattgacc tcgtcatcgt
     4861 gggcagcgac cgcaccctgg gccgcacggg cgaagtcgcc aacaagatcg gcacctacac
     4921 caaggcggtg ctggccgggc ggcacggcat tcctttctat gtggccatcc cgctttccac
     4981 gattgattgg gagctgaagt ccgggaggga cattccgatt gaggagcggc acgaggagga
     5041 ggtcttgggc gcgtggggcg tgccggcagg cgcgggccgg gcacgagccg ggcggcgcgc
     5101 ctatgtgcgc gtggccaatc ccaccagcgg ggcgctgaat cccgggttcg atgtcactcc
     5161 ggctgagttg atcgccggca tcatcacccc ggtggggatc ctgaagccga gcgagctgtg
     5221 ggcgcgacgg cgagaactgg gttgggcggg gtgagcccgg cttagcggga tttccagaac
     5281 tgccaccagc gcttgcgggc gcgcgggttc ttccactgct cgtagcgccg ggtgatgacg
     5341 gcggcgattt tctcgtagtt ttcccaggta tccgccacgt gccagaagct tggcaggcgc
     5401 gcggccagcg tcgttttgta atcggcgaag tattcgccgc gccgcccggc ttcgtccacg
     5461 tagtaatact gcacgaactg gttgccctcg tcgttcaggt ccgtttccga gaatcgtccc
     5521 gggcaggcgg cttcgaagaa ctgtcggcca gtgatctcgc gccgctgcaa gcgcgccagg
     5581 agcgccgcgg aatgctggcg gtgaaagtcg ctcaccaatc cgtgctgcac ggcccaggcg
     5641 aggaacatgc cgatatgcgt gccggcgtgt cctttgggca gcgggtcggc atcggtgctg
     5701 taatcaaagt ctgcgcggtc gtaggacatg gcggcttcga ttatggcagg gggcgtagcc
     5761 ggacgcaacc cgggccgtcc cggttaaagc aaccggggtt gcgccttttc ccgggcgagc
     5821 ccttcggcca ggccggggcg gtggccgttg cggcgttcga taatgtgttt gtccaccgcc
     5881 gggtcgaaat gaaccgggta atggccgtcg tagcaggcca tgcagaaggc gttggccggc
     5941 aggccggtgg cctggagcag gccggcctgg gagagatagt tcagggaatc ggcgttcagg
     6001 tatgagcgaa tctcctccaa cgaatagttt gccgccatca gcttgttgcg atccgggaag
     6061 tcaatgccat agacgcaggg atggcggtgc ggcgggcaac tgaccagcac atgcacttcc
     6121 cgcgcgccgg cttctttgag gttgttgacc cgggtccggc aggtggtgcc gcgcacgatg
     6181 gaatcgtcca cgacgatcac ccgtttgccc tttaccagtt cttcgatcag gttgagtttg
     6241 acgcggacat cgaagtcgcg gatcagttgc gagggctgga ggaagctgcg gcccacgtag
     6301 tggttgcgca cgaacgccat ctcgaacggg atgcccgatt cgagcgagta gccgagcgcg
     6361 gcgcagttgc cgctgtccgg cacgggcacg acgatgtccg ccgcgatcgg gaattcgcgg
     6421 gcgagttggc ggcccatctc gacgcgcacc tggtaaacgc tgcggccggc aatggcgctg
     6481 tcggggcggg cgaaatagac gtattcaaaa atgcagaagg cgcgctgtgt atggtccgga
     6541 aaggcctgcc aactggtgag gccgtcgcgg ttaatggcca cgatctcgcc cggcgccacg
     6601 tcgcgcacgt attgggcgtg aatcagatcc agggcgcagg tttcgctggc cagcacccag
     6661 gcgccatcaa tccgcccgag gcacagcggc cggaagccgt gcgggtcgcg cacgccgatc
     6721 agctcgcttt cggtcatgat gaccagcgag taggcgcctt cgatgcggtg caggctgcgc
     6781 acgaggttgt tgggctcgcc ctgctgggtt ggctgggcca gcagatgcag gataatttcg
     6841 ctgtccaccg tggtttgaaa gattgagcct cgggcttcca gttccgcgcg gagccgggct
     6901 gcattggtga gattgccgtt gtgtgcgatg gcgatctggc cgcgggcaca atccaccgtg
     6961 agcggttggg cgttgcgcag gtgcgacgag ccggtggtgg aatagcgggt gtggccaatg
     7021 gccatgaggc cgggcatttc ctgcaggacg tttccccgga aaacctgcgg caccaggccc
     7081 atacccttat gcgtgtagaa ctgccggccg tcggatgtga cgatacccgc gctttcctgt
     7141 ccgcggtgct gcagcgcgta aaggccgtag taggtcaact cggcggcttt agggtggcca
     7201 tagacgccaa agacaccgca atagtgttta ggataagttt gcatggagcc ggcttgcttt
     7261 tctcagtgca tggcctcggc gatagcattc caccagcggt cgtggagatc ccgcacggga
     7321 cagttcaatt cagctcctgg gacctggagc cgcaacgcgt cgccgcccac ggtgccgagg
     7381 cgcgccgtgg gcacgccgag aatccgggcc tgggcgagga ccttggcggc attgaccgcg
     7441 tccaccgaaa tcaggattcg gccctgggtt tcgccgaaca gcagcgcatc caggcggatg
     7501 ccgtcgcgcc cgggggcttc ggtcagcgga gtgagatcca cctgcgcccc gatcaggcgc
     7561 ggggttgcgc gcgccaccga ctggctgatg cagccctcgg cgagcgcgac cgccaggccg
     7621 ccttcgctgc aatcgtgggc gctcttgacc acgcccgaat gaatcagcgc ccgcaaggcc
     7681 aggtgcaact ctttttcccg ggccaggtcg caccgcggcg gcgtgccatt tttcatgccg
     7741 tgaatcaact gcaggtaggc cgagccaccc aagccgagca acgggtcgct cgtgtccacc
     7801 aattcaccca gcagaaggat gacgtcacct tcgtctttga accattgggt ggtgatgtgc
     7861 tcggggtttt cgatcaggcc gaccatggcc actgtggggg tggggtcaat ggggccgttc
     7921 ggatgctggt tatacaggga gcagttgccg cccgtgaccg gggcgttgaa cgcgcggcag
     7981 gcgtcagcca ggccgcgcac gctttccttg agttgccaaa acagctccgg gttatgcgga
     8041 ttgccgaagt tgaggttgtc cgtcgcgccc agcggcatcg cgccggtgca ggcaaggttg
     8101 cgcgcggcct cggccacgac aatcttgcta ccttcgtagg gatcgagata gacgtaggcg
     8161 ccgttgccgt ccacggagag ggcgataaac ttctcgggca cgggtcgcag agcggaggcg
     8221 gcgttcgggg gcgcgggcac cgagtcttcc ttgattcgca gcaccgcggc gtcgctgccg
     8281 gggccgacga ccgagttgtt gcgcaccatg tggtcgtatt ggcggtacac ccagtttttg
     8341 gaggcgatgg tgggccaggc cagcaacgcc aggagatgcg ctgccgggtt ggttgtatcc
     8401 ggcacgccgt cgaggcagaa ggcgcgcacc gcttgcaaat actccggttc ccgggcgacg
     8461 cgttcataca cgggggactc gtcggcaatc ttgcgggccg gaatatcggc cacgacctgg
     8521 ccgtggtgcc ggacgaccat gcggcccgta tcagtcacca cgccgatctc cgcccaaggc
     8581 aagtcccact tgtcgaaaat gcgttttacc tccgcttccc ggccccggtg aacgatgatg
     8641 agcatacgtt cctgggattc gctgagcatg atctcgtagc tcgtcatgcc gggcgcccgc
     8701 tgcggaactt tatccaactc gatctcgatg cccgttccgg cccgcgccgc cgtttcgcag
     8761 gtcgagcagg tcaggcccgc cgcgcccatg tcctggatgc cggcgaccgc gcccgtggcc
     8821 agcagttcca ggcaggcttc gcacacgagc ttctccatga acgggtcgcc cacctgcacc
     8881 gctccccgtt gttgctcggc ggattccgcc gtcaggtcct gcgaggcaaa ggccgcgccc
     8941 gccagtccgt cgcgcccggt tgccggcccg acgtagaaaa ccgggttgcc cacgccgcgg
     9001 gcatgtccgc ggacgatttg tccgtggcgc agcacgccga ggcagaacgc attgaccagc
     9061 gggttgcctt cataagacgg gtcgaaataa atctcgccgg ccacggtggg gattccgaag
     9121 cagttgccgt agtgtgcgat gcccgccacc accccgccaa acaggcggcg gttgttggcg
     9181 acggccggtc ccggttcccc ggcggcggtg atcggtccga accgcagcga gttggcggcg
     9241 cataccggcc gcgcacccat agtgaaaatg tcgcgcacaa tgcctcccac gccggtggcg
     9301 gcaccctgga aaggctcgac ggcgctgggg tgattgtgcg actcgatttt gaaggcgatg
     9361 gccaggccgt ccccgatgtc gaggatgccc gcgttttcct cgccggcgcc caccaggatc
     9421 cggggcgatt gggtggggaa ggtcttgagc agcgggcggg tgtttttgta ggagcaatgc
     9481 tcgctccaca tcactgagaa gatgcccagc tcggtgtagg tgggcacgcg gcccagcacc
     9541 gctttgagct tctcgtattc ctcgggcgtg aggttgtgct tttgcaccaa ctcgggagta
     9601 atggcggggt cagtcatgag gattccaaaa gcgggaggat gtggttgtgt gggcggtcaa
     9661 aagcgcgggt ttacccggcc gctgtcggcc gggacgccag ggccgcgatc atgctttcga
     9721 ggatcaggcg gccgtcggtg ctgcccagga tgggctcgct ggcgcgctcc gggtgcggca
     9781 tgaggccggc tacgttgcgc ccctcgttgc agatgccggc gatgtggtcg agcgctccgt
     9841 tggggttggc gtcggcggtg atctcgccct gggcgttgac gtagcgccaa aggatctggt
     9901 ggtttgcctt caaccgggcc agggtgtccg cgtcggcata atagcagccc tcgccgtggg
     9961 cgatcggcag ccgcagcaga ttgtccggcg gaatccggtt cgtgaacggc gagtcgggag
    10021 tggaggtctt caggaagatg tgttcgcagc gaaactgcag cgagcggttg cggatcagcg
    10081 ctcccggcag cagcccggct tcgcatagaa tctggaagcc attgcagatg cccagtacca
    10141 acccgcccgc tgcggcaaac tgccgcacgg cttccatcac cgggctgaat cgcgcaatgg
    10201 cccccgtgcg caggtaatcg ccgtagctga agccgccagg caccaccacc gcatccgccg
    10261 cgcccaggga cgcttccttg tgccagcgca gctcaaccga atgccccagc acgtggcgca
    10321 gcgcgtgaac gacatcctga tcgcagttgg agccggggaa ttggaggacc gcaaagttca
    10381 tcggcacagg cctccttact cgatctcgaa ccggtaatcc tcaatcaccg ggttgctcag
    10441 gattttgtgg caggcggcgt caatttgctg gcgcgcggct tccgggtcgg agttcgtcag
    10501 ctcgatttcc aggtatttgc ccacccgcac ggcgccgatc ccgtgatagc ccatctgggt
    10561 cagggcgttt tggatggctt tgccctgcgg atcgagcacg gcttgcttcg gcatgactac
    10621 aatttttgct ttcatggtag aaaaggaggc tccactacgg aagccgcggg aaatgtgaac
    10681 cagttgccgc tttgaagcca gagttttttt gtcccgtacc ggagtgcgct gcaattacgg
    10741 attggcaagc ggcgcgggag ggtttacata aagctcgaaa accgaatgat tacgcccgcc
    10801 accgcccatc gcccgcccgc cacggaatga acctgcccaa ccaactgacc atctcccgct
    10861 ttctgctgac catcgccttt ttgacggtaa tgttttccgc gatccagttt caccgcacga
    10921 ttgccctcct gctcttcatt gccgctgcgc tcagcgacta cctggacggg gtgatcgccc
    10981 ggcgccgcaa gctggtcacc aatttcggca tcctgatgga tccgctagcc gacaagatca
    11041 tggtttgctc cgccttcatc gcgtttgtcg ggctggggtg gatgccggcg tggatggtgg
    11101 ttatcattgt tgcccgtgag ttggccatta cggggatgcg cttgctggcg gcttccagga
    11161 acgtggtgct ggccgcggag ggctgcggca aatacaagac cgtcgcccag atcgtcgcca
    11221 tcatctccat ttttgtggta gccagttacc cggaatgggg cgcggtgggc cgggttgttt
    11281 ttggctatcc gtggcccggc ccaaccccgt gggcgcaatg gttcaccgag ttggccaaat
    11341 gggttgccgt ggggctgacc tttctctccg gctggcttta tctctggcgc aatcgcgcca
    11401 tctaccttca ggacctgtga aactttggat tgcgcaaggc ttcggcgttg gccgcattcc
    11461 cctggccccg ggcacctttg gttcgctgct gggactgctc tggttcgggc tgctgctcct
    11521 gacagggcat ctttggcttt ttcttgccgg taccctcgct gccctggccc tctccgtctg
    11581 gctttgcggt gctgccgagc ggtccctggg gcagactgat cccgggagcg tcgtgttgga
    11641 tgaaattgcg gccatgcccc tgtgttttct cggctggatg gcggttcaat tctggcacac
    11701 cggggcctgg cccgcgctat ccgcattttt ctccgcgcgg acctggcctc tgacgctggc
    11761 ggtctttgcc gcgttccgct tgttcgatat tctcaagccc tggccggtgc gccagagcca
    11821 gtcattgccg ggggggtggg gggtgacgat ggacgacgtg ctggctgcgg tttacgtcaa
    11881 cgtgatagtg ctgctggcca ttgccctggc cgcgcggttc tgattgccgc cgcccacggg
    11941 agccggggcg gttttcctca acgcgccggg ctgcgcggtg ctggcggcag cggcagcgtt
    12001 tctccccaat tgtccggggc ggatggatac cgtccgggtg ataccttctt caacggatca
    12061 tgatgccgcc aggcgaccca gccccagagc gccgcgataa agagcgcgac ggctcccagg
    12121 gcgatcagcc attgacgcca cgccacctgc gaaagctgca acatcaggaa gtctgccagg
    12181 ttgcgcgggc gtttggccag cggcggcggc tcggcgaact tggcactgcg ctccagttcc
    12241 tgatccaaag ccaccatgat ttgcggcaca tccagcttga gcaaactcgc ataactgcgc
    12301 acaaaaccac gcacataaac cgtcgcggaa aacacctcat aattcccctc ttcaaccgcc
    12361 cggatatggt cgctccgcac cttggtgatc tccgccacct gttgcaccgt caggctgcga
    12421 gcttcccgcc cctgccgtag ttgttctgcg actgtggaca tgttccccat cttttaacgg
    12481 ttttagcccc gcttgacaac catgcaggca acgcatcctg cagggaaaga cgccccggac
    12541 ttttgcgccc cgaatcttca gccgcccacc cgatcgctgg cgtggcgggc gatccatttt
    12601 cgtgaccact ctaccgctgc caggttcatg tcgcacaacg gcgtgcaatg aaagatcagg
    12661 ccttcgatct gcccctggct cagccactgc cagcccagac ggcattgatg ctccatggct
    12721 tccgtcgaaa tcggttggcc ggcgccaaag tcccacatgt aaatccccag ccgcgtccgc
    12781 ccggttgggt tgatcttgcg ataggcggca aagttccgct ccagggctga caggtcccga
    12841 gcctgccacg tccagaaata caccacgtcc acgttctcaa gatgccgctg gattgccggg
    12901 tgcagttggt gggagtagag caccagggac aattcgagcc ggcgtttgcg gtttgccagt
    12961 tcctgctgaa tctgccgtac ctgctcgacg ctcagatgcg caggggcttc tccgccttcg
    13021 gtctttggaa ccgcgttgcc gaggaagaaa tcatccatat ccaacccgac caggtttggc
    13081 agcttcgcgg ctaagccgag cgccaactgc ttcttttgct cagtggtatg cccgctgtct
    13141 ccgtccacaa agctccacgt caatcgccgg gcgtgggcga attgccgggc gtagtcctcg
    13201 aacggtggtc gcggcgtgcc cgtaaagcgc accatgcaga cattgggaat gcccatatac
    13261 ttgattgccg ctcccgggag aatgtctccg ccctggctgc caatcccaaa cgagttcttc
    13321 aacgatccgg catcgtgccc ccacatccac aaccggtcgc gcagtttgcg cccgaagggc
    13381 cggatgtttc ccaggcaaac tgttggctgc ccaggcattc caggcagggc gcaggcgcct
    13441 gccaccatcg ctgtgcgggc gagaaatctt cgtcgggtca ggaaagagct ggcaggcggg
    13501 tgcatgggag cagttcctat tctatcgctg gggggcgagt aagaaagcca aagacggaca
    13561 tcgtgctcat tctgtagcaa ataatcacat aatgggcggc tgcgacaagc taattaaggc
    13621 ttcttttgtt caccctgcaa ccgctaatcc gggaacgtaa aaaggctgcg gacgggatgc
    13681 cggcctcgtg aatcactcta tgcggccgct gggcaagctg atccgctttg gcggagatgg
    13741 gagatgccgt ttccggagtc cggcataagg gtgagcaggg ctggttctgc cggggcgttg
    13801 ctggcgatgc gttcccaatc cagcaaggag atttcgggag gcttcggtgc ctggatagat
    13861 ttgaggctta cggtttgtcc tgccgcgccg tgggcggctg ctgaagaatg tcaattcttt
    13921 gccgatgatc cacggttggc cagtccaggc gataaaggca atcgaagagc cgctccttaa
    13981 aagaggccgg gccggtggcc ttttcggcag cgatttcagc ggcaataccg aagcacgcca
    14041 gagcgcaggc ggcggccagc gtcgcatcgt gttccaccgc cgcaaaagcc ccgatgatcg
    14101 aagccgccat gcaaccggtt ccgaccacgc gcgtcatcag ggaatgaccg tttttgacga
    14161 gataggtgct tttcccgttg gtggcgatgt cccattggcc ggtgattacg acggtggcct
    14221 ggcgttggtc cgccaatcgc tgcgccagcg agaccaggtc ggcgctaatt tccgtcgcat
    14281 ccaccccccg ggttttgacg tccgcgccgc tgatcctggc gatttcggaa gcgttgcctt
    14341 tgatcacggt taccctggct tcgttgagga gttccagact ttttcggtcg cgcaaggcgg
    14401 ttgcaccggc accgcaggca tccaaaacca cggggatgcc tttctggtta gcgctccggg
    14461 cggccagttt catggcctca accagatcgg ttgtcaaggt gccgatgttc aaaaccacgg
    14521 cagaggcgat ttgggccatt tcagcggctt cctcgcgcgc gtgagccatt accggcgatg
    14581 cccccagggt tttcagcacc tgggcgcagt cgtaaatggt cacccagtta gtcaggtgat
    14641 gcaccacggg ggattgctgc cggattttct ccagcagaac gaaggtattg gggctggccg
    14701 tcatggggga aaggagaaaa ttcagcttgc cgaaagctct ggattacaag tcgccgccat
    14761 ccacctcgga ttcaacagcc tccagaaaag ctttgagagc cttgacgcgc ggttcggcca
    14821 gacggtgcgc agatggcagt tcaagcagtg taggcaggcg ctccaggttg caccgcagag
    14881 cctgaacagc gtcggcatgg cgcacgaacc tcgtgtcgcg gcctactttg ctgacgacgc
    14941 ggagaaccgc gacggctccg agttgttcca gcaaatcggc atcccgcagc agcgtgcctt
    15001 caaagctggt cggctggcac gtgggcaagt gcgtccggac agcttcaagg gttgcgggaa
    15061 tcttgtcctc cgggaacccg aattgccgga gaatcgcagg cacttctctg gccgcatacg
    15121 ccacgtggtc ccaggccgca agcgccgccg gttcttccgg ccgatgcccg acgaagaccc
    15181 cgaggtcatg catccaggcc gccgcataca ggatgtcgtc atcgtagggt ttgtcttctg
    15241 ccagccgccg agcgaggcgg tacagccgcg gttggtggct gaacttatcc ggcggctttg
    15301 cctgggcgcg gatataatca gcgatcgcct cgcggtatcc gggttgaatc atgccgcagg
    15361 atagcagtgc cgccgtcaac tctgtcaagc gccgcacatt gatcaaggta agcgcgagtt
    15421 gccaaccctc cgaggctaat tccaagggta aatattcccc gcccactcgg gtcaaggtgc
    15481 gggacctgac aaacgcaacg gccgagttcc tgaagagctg acagggttgg cccgggaaag
    15541 gcgtcccagg ccgggcgttg gcatcagcgt ccgctatcct ggggacgcag ttcaaaacga
    15601 agatgcgcct tgaaccgaat cgcagctaaa aaagagcttg cgtatttatt tgggaactgc
    15661 cagtctctgg atgtctgtag atagcttgtc aatcccatgg catggtaaat cttcaggtga
    15721 actcagttca gtgatgattt gaaacccgat gagcgggaac agtctggcga gagcaatagt
    15781 tagtctagga cagtagttag agaatcgaat gagtgcaaaa ctgtttgtag gaaacctttc
    15841 cttcaacatc accgaaaacg atctgcagga cgcctttgcc gtccatggca cggtcgtcga
    15901 agccaatctg atgatggacc gggccacggg ccgtccgcgc gggtttgggt tcgttaccat
    15961 gagcacgccc gaggaggcgg aaaaggccat tcaggccttg aataatacgg aattggcggg
    16021 cagaaaactg accgtcaata tcgcccggcc tcgcgaagaa cgcccgattg gcggctcgcg
    16081 tggttacggc gggcgtggtc gcgagcggta ttaatttaat cgcttgcttg gattcagggg
    16141 cccgggcgca ccgggcccca ttctttttgc cggcggcggt tattgcagcc ggagaggagg
    16201 gtaggttgcg ggcggtgcgg gtgaacaagg cgccaatcgc cggccgcggt agcggcacgc
    16261 gttcatgcga gcgcagcccg gtctaatcag gcaacaaatt ccagttccgg ttgccggggg
    16321 atcagtccgc gcgccgccgc ttgccccagg aaccgccgga tggactcgcg gcctttttca
    16381 ccgtagtcga gcgtccagtg gttcacatac atgccgacga acttgtcggc caggtccagg
    16441 cccatgtcgc gggcgtattg cagggcatgc tgtaccgctg caggccgatg atcgaggctg
    16501 tattggatgc tggctgtcag cacatccgac accgtgcggc gtatggccgg cgggaagcgc
    16561 ttgtggatga cattgccccc caggggcagg ggcaagcctt cgttggttcg gccccaccag
    16621 gcgcccaggt cctcgcacat ttgcaggccc tgctgctgat acgtgagctg gccctcgtgg
    16681 atgatcaacc caacctcggc ccggccgtcc cggacggcgg cgaagatttg atcgaacggg
    16741 accacaaggc agtccagttc gcaggcgggc tggttcagcc atagctgcaa cgcgaggaag
    16801 gcgctggtca tcgtgcccgg cacggcaatt cgtttgcggc ggatctcggc ggccgaaagc
    16861 ttgtccctgg ccacgagcat ggggccgtag ccgtcgccca tgctcgcgcc gctcggcagc
    16921 agggcataat gcccgctgac gtaggcatag gcgtgaatgc tgatggccga gatgtcgagc
    16981 tcgccgcgcg tggcccgttc gttgagggtt tggatatcct gcaggatatg ctggaaacga
    17041 aagccgccgg ttggaatcaa gtcgcgggcc aacccgtaga acatgaacgc atcgtccggg
    17101 tcgggcgagt ggccaagcgt aagagtgttt tgcgacatgc gcgcaggcta ctccgttttc
    17161 cgcgcggttg tcacgccgag aagttcgccg cgcttttgcg cccgggtcgc cgggtgcgtc
    17221 caatccgctt gtcttcgcct gcgcgcgccc taaactgcgc ggcgatgggc catctggaca
    17281 cgcttaaccc gcagcaaaga ctggccgtgg agacgattca cggcccggtc ttgatcctgg
    17341 ccggcgcggg aaccggcaag acgcgggtga ttacgcatcg cgtggcgcat ctcatcgagc
    17401 gcgggattgt ccccggccac attctgggcg tcacattcac caacaaggcg gcgcgcgaga
    17461 tgcaggagcg ggtcgcccaa ctgatccgca aacggctgcg aactcccggg ggcgaggcgc
    17521 cgggcggctc cccggcctcc gcgcccgccg cggccccttt gtcccccacc atttgcacct
    17581 ttcactcctt gtgcgtgcgc attttgcggc agcacatcga gaaactcggc tacaagcgca
    17641 acttcgtcat ctatgacgaa gcggagcaac ttggcgtcct caaaaagatc ctggcgcaga
    17701 tttccacccc ggatgcgaag gctgatcccg cggcaattct ggcctggctc agccggcttc
    17761 gcaatggcgg cgagcatgcg acggcgttgt ccgaccccgg ctggcgcgcc ctggccgagc
    17821 acctccgcgc ccgctacgaa tcggccctgc gcgcctgcaa tgcggtggac tttgacgatt
    17881 tgattctgct cacgctgcgg ctcttccgcg agcagcccgt ggcgctggaa gcctgccgcg
    17941 cccgctaccg ctacgtgatg gtggatgaat accaggacac caacgccgcg caattccgcc
    18001 tggtccatga cctgacccgc gagcaccgca acctttgcgt ggtcggcgac gacgatcaaa
    18061 gcatttacgg ctggcgcggc gcggaaatcg ccaacctgct cgagatcgag aagcattacc
    18121 cggaaacgcg ggttatcaag ctcgagcaaa actaccgttc caccaacacg atcctcgccg
    18181 ccgccaacgc ggtcattcga cacaacgtcc ggcgccgggg caaaagatta tggtcgcaaa
    18241 aaggcgatgg gccgaagatc acgttgcatg cttttgccag cgatgaggag gaagcgcgca
    18301 ccatagccga gcagatcgag tttgcccggc tcgcccaccg cacgccctgg gcccaccagg
    18361 caattctctt tcgcaccaat gcccagtcgc gcccgctgga aaccgccctg cgccaggccg
    18421 gcgtgcgcta ccgcctggtc ggcggccaga gcttcttcga ccggcgcgag attcgcgact
    18481 tcctggcgta cctgaaggcg ctgctgaatc cgagcgacga cgtgagcctg ctgcgcatcg
    18541 cgaacgtgcc ggcgcggggc ctgagcgacg ccacgatgga gcgcctgctg gcggcgagca
    18601 gcgagcgcaa atgcccggtc tttgcggcaa tgaagaaccc ggcggtgacg acctcctttc
    18661 cctcccgccc gcgcgaaagc atcgcccgct ttgtcgaact catcgagcgc acgcaggagc
    18721 agctcctggc cccgcgggag cccggggcgc gcgatccccg ggcgctgcag aactgggcca
    18781 acggcttcct gaccgagatc ggctacttcg cggaaatacg ccggctcgaa aagaaccccg
    18841 aaaccgcgga gaaccggctc cggaacctca aagacctcat cgccactctc gacgaatccg
    18901 ccagcgccgc gccgtcccgg ttcgagggcc tgcagacatt tctggagcag atcaccctgg
    18961 acgccgggcg cgaggaagaa aaagaaccgg acgcgaatga cggggtcaca ctcattacga
    19021 tgcacagttg caaggggctg gagtttccgc acgtttacgt ggtcggcatg gaaaacggac
    19081 tgctgccgca ttcgcgcgca caggcggacg gcacgctcga cgaggagcgc cgcctgttct
    19141 atgtcgccat tacccgcgcc atgcaaacgc ttaccatcag ccattgcggc gcgcggaaaa
    19201 aatacggcac ccctgtgccg tgccatccct cgccgttcct ccaggacctg ccgcccgaat
    19261 tggtggagca cgccgacgag cgggcccgcc agcccgtcac gccgcaatcc gccaaggcca
    19321 acttcgccat cctgcgcgcc tccctggaca acgcggagcc tccaccggcc ccgtcacgcg
    19381 ccgggccgaa ccgggcgcgc ggagaaacgc ctcgctagtc aggggtggcg gacggttgct
    19441 tccggggcgc ggcctttgcc cgcccgggcg cagccgatga gctgccgcac gtccgcgagg
    19501 ttttcctggc gcataaactg gcgcaggccg gcgatgattt gcagggcggt ttggggggcg
    19561 tagaagttcg ccgtgccgac ggcgacggcg gtggcgccgg ccatgataaa ctcgagggcg
    19621 tccgcggtgt tctgaatgcc gcccatgccg atgatgggga tccgaaccgc ctgggcggcc
    19681 tcccaaacca gcttgagggc gatgggcttg atgcacgggc ccgacaagcc gccggtgacg
    19741 ttggccagca cgggacggcg ggtttcgaca tcaatagcca tcgccggata gctgttcatg
    19801 atggacaggg catcgctgcc ggcggcttcc gccgcctggg cgtaggggac gatgctgacc
    19861 acgttcggac tcagtttggt aatcaacggc agccgggtcg ccgcccggca cgcggccacc
    19921 acctggcgaa ggagcgcggg gtcggtgccg aactgcgcgc cgccttcctt gatgttgggg
    19981 caggagacgt tcaactccaa agcaccgacg ccgccgaggg catcgaggcg gcgggccacc
    20041 tcggcgtatt cctcgacgga ggtcccccag atgttgatga tcgtgggcac tttcaactgc
    20101 tggagaaacg gccagtagct gcgaataaag ccgtccacgc cgggtccttg caagccaatg
    20161 gcgttgatca ggccgctggt gacctcgacc agccgcggcg tcggattgcc gcggacaggg
    20221 tcgcgccgga ttcccttaac gaccaccgcg ccgagctggt tcaggtcaag gagttgggcg
    20281 tattcaacgc cgtagccaaa ggtgcccgag gccacggtga ccgggttggg cagcgccagg
    20341 ccgccgattt ggacggccag gttcattcgg ccgcctccca ggcaatttgc cgaaagtcaa
    20401 agaccggccc ttcggtgcag gttcgctggt attcccagcc gccggcggtc ttgatgggga
    20461 ccacacacgc caggcatacg ccgatgccgc atcccatgtg ctgatccagc gagagttccg
    20521 ccggcaggcc gtgctccgcc gccagccggc cgacggcccg cagcatcggc gtggggccgc
    20581 aggcaaacag tttccgcccc ggcgcgcgcc gctggagctc ggcccgcaag ggctccgtca
    20641 ccacgccttt ctcgccgtgg ctgccatcct cggtcgtcac gcgcacctcc caaccgagcg
    20701 cgcgaaactc cgcctcgcaa agaatgtcag cgcgctggcg tcctcccaca aagaccaggc
    20761 ccttccgggg cgatcgttgc gccagcaggt agagagcggc catgccgtag cccccggcga
    20821 ccaggagggg aatctcgccg tccggacgcg gcaccgtgaa gccgtgaccg agcgggccga
    20881 ggaggctcag ttcctcgccg ggccgcatgc gcgccagcgc ttcggtgccc tggccgatgg
    20941 tcttgtagag caaggacaag gtatcccccg ccacctggaa aacgctgaag ggccgccgca
    21001 gcagcgcgtg cttgaggggc agaatccgga catgcacgaa ttgccccggc tgcacccggg
    21061 gcgcaatcag cggggcgcgc acccgcaggc ggaagtaggg gccggtgtcg cgctcattgg
    21121 cgataatctg ggcggtttgc tcaaccatgg gcgcgcaaac gggaggcggg gcggcctggg
    21181 cttgtccggc gcactgcttc ggcgcgcgag ccgcaccgcc gcgggtcatg cccggcgggc
    21241 ggcgctgtcg ggcgccagca gttggcgata agtggccagc gccagcagcg gccaggagtt
    21301 tcgatacatg tcatatttga ggtagaatac cttcgggaag cccgtgccgg tggtttcatg
    21361 ctcagtccag gagccatcgg cattttgcgt gcggaccagg tattcgattc cccgctggag
    21421 gctggggcgt tccgggtcgc caaaggcgca aaggcccatc accgcccagg cggtctgcga
    21481 ggcggtgctg gggccctggc ctttgaagac ggggtcgtcg taggtgttgc agcgctcccc
    21541 ccaaccgccg tcctcgtgct gcacgctctc cagccagtcc cgggccttga gcagccacgg
    21601 ctggcgcata tccagattca gggcccgcag cccgcgcagc acctgccagg tgccgtagat
    21661 ataattgacc ccccagcggc cgtaccacga gccgtcttcc tcctgccgct cgcgaatgaa
    21721 ccgcagcgcc cgctgcacct ggggatgctc cacgctccag ccttcgtagc cgagcaactc
    21781 caggatgcgc gccgtgatgt cggcgcattc gggatccagc atggcgttat ggtcggcaaa
    21841 gggaaccttt tcgagcacgc ccttggtgca gtccttgtcg aaagccgccc agccgccgtc
    21901 cttgcattgg aaggtcatca tccaggtcag gccccgttgg aaacattcgt cacgtttctg
    21961 ccggtcgtct gtcgccacca gccgcagcgc cagcagcacc atcgctgtgt catccacgtc
    22021 cgggttccac ttgttgtcga actcgaacac ccacccgctg ggctcgacct tggccgggtt
    22081 cttgtaaatc cagtccccgc ggaagcggat ttcccgggcc atcagccact cggcggcgcg
    22141 tttcaacgcc gggtgatccg cgggcacacc ggattcacgc agacagatgc tgacaatggc
    22201 agtatcccag accggcgaca ggcaaggctc gatgcggacg ctctgctcgg tttcgtgctc
    22261 gagtttcttt aattccgccg ccgcccgcac cacctgcggg tggtcgtccg ggtagcccag
    22321 cgctttgagg gcgatgaggg aattgagcat cgccgggaag atcgccccca ggccatccga
    22381 cccctcgaac cgttccagca tccactgttc ggccttgcgc aacgcccgct tgcggaaggg
    22441 atggattccg tgctgcgcga accattccgc gaacttgtgc acccggtcca gccagagaaa
    22501 gaagttgcgc cacgtaatcc gttccgggtc gggcgccagc gccaggtcgc gcccgtggat
    22561 gccctccggg taaagctcgt ccagtttgac cccgctcttc agtttgcggg tgggcttgaa
    22621 atgattgatg atcgccagcg gcacgaacat cgagcggctc caggagctca tctcgttgaa
    22681 gttcacgtag aaccatttgc cgatcaggat cacttcgcag gggatggtcg gcacgtactc
    22741 ccagggaaac agccccagca aggccaggta cagcttggaa aaggtgttca tgcgcggcac
    22801 ccccccgagg ttgaccgcca cctcgcgggc gcgcagcatg cgggggtcgg tgacgggcac
    22861 cccggccagc ttgagcgcga gatacgcctt gacggtcgcg ttcacctccg ccgggccgcc
    22921 gtggtagata ttccacccgc cgtcgggcag ttgcttggaa aagatgtggt tgaccgcctt
    22981 gcgctcccat tgcgggtcca cctggccgtt ccaatgatga taggccacca tgtccgagac
    23041 cagcgtggca tccaccagca actcgccgat ccagtaaccc tcggctttct gctcgccgag
    23101 caaataccgc tgcgaccgct caatggcggc ttccaactcg acccgaggct tgaacccgaa
    23161 gtctgtttga cgcgggtttc cctcagcacg gctttggcga agcgatgcta cttccatccg
    23221 cttatcctgc ccagtccaga ggcgggtgta aagctttcat tcgcccgcgc tgatcggcgg
    23281 cgccccaacc gcggcccctt tgcgcccgcc gcgggcggcc gggagcaacc ggagcgcgcg
    23341 gcccacctcc ggccatcagc ccgctcaacg gtagcgcagg ctgctgaggt agccccaggc
    23401 aatgcccatc accaggcccc ccgcatgggc cgcattggcc acgtgcggca cgatgggcgt
    23461 gaaacaggcg aagaaccaga tgatcatcat cgtcagcgtc gtgggatgca gatacaggcc
    23521 ggacccggga tcaaatttgc cccgcatcca aatgtagccc aacagcccat agaccacccc
    23581 cgacatgccg ccaaacaccg gccccccgaa ccagaattgc accaggttcg ataccaccgc
    23641 cagccccagc acaaggcggg ccagcatgcc cgtggactgc cgcccctcaa tcatgctccc
    23701 caaatcgtgc agccacagca ggttgaagag tatgtggagc aggttgaaat gaatgaagat
    23761 cggcgtcacc agccgccaca cctggccctg gaggacttct ggcagacccg ccccggcgga
    23821 gcccccggca acgaagttcc ggatgaacag cccctgaatc ggccggaaat ccttgcccat
    23881 gccggacaaa atgaaaaccg tgacgctgat gaccatcagc gcaaaagtca gcggcccgaa
    23941 cccgtaggcg gtcggtgctt gaaagagatg ccgccgttcc tgctgtttct tgcgataccc
    24001 ggcctcgcgt ttctcggcct cggcgcgccg gtcagccgcc tcccgggcct gggcgcggta
    24061 ttgcggatcg gcgggattgc tgcggaactc ggtcagcagc cgctccgcat cctggatctg
    24121 atcctcgtcc gccacccaaa tgccccagcc ctccgcgggc tcgaactcga gctgcgtctc
    24181 gatttgccgg acatagagaa aatccgcaaa cgtgcgggcg gccttttcct ccgccaggta
    24241 gccgattaaa cgcatgccgt tggaagcggg gcccgggccg aaggccgggc gctacgcggc
    24301 ccgccacttc agctcgacga cctgcccgtg cgcgcggtgc gcgggatcca tgctccgcaa
    24361 ttcgagccgc atcccgcgcc gcttcaaggt cgccagcacc cacccggaag ggtcttccgg
    24421 gcggaagaca tagcccagcg ccggcaggtt gatcaggtga atgccgctgg ggtcctgctc
    24481 cacgtgccag gagtgggtgt ggccgtagat atacgccttc acctgtttgc gcgggcggat
    24541 gatgttcaaa aacgcctccg aatccttgag gtcgctgcgt tttgccgtcg tgcccacatt
    24601 atggtgagcc aggattaacg tgggcttttt cggatggtcg tccagcgtcc gggccagcca
    24661 atccaactgc tccggcccca gcaatccggg cgtggacagg gtcttttcga gcgagtccag
    24721 gataatccag ttcacgcgcg gcgtcggcag cagcgcaacc tgccggtcgg ccagcggtcg
    24781 cgggggcgcg cccccggctt ccaggaccgc ccggaaacgc tcgcggttat catggttgcc
    24841 catggcgatg tagaccggga cctgtccctg ccgcaacggc tgcaacaggc gcgccaggtg
    24901 ctcataatcc acggattgcc cgctgttaaa ggcgcaatcg ccggtgatga tgacccccgc
    24961 cgggcgcttg cgcagggcca gcacctcgcg cgtggcctgg gtgaaatgat ccgtcatgtt
    25021 gatcccgcgc tgaaccatgc tggcatccgc ggacaggtgg atgtcggaca gcagcgccca
    25081 gacattcggg tcccggcgtt gggacgcggc caacagctcc ggcgccagca ccagcgccgc
    25141 gctgccaacc agggtgcgaa cgagaaactc acgacgtgaa agagcaggca aatgtttcga
    25201 catggtctct tcctaccgcc gcccgccggg tgactcaagc caaaacgttg aggaacccgc
    25261 cggaaatctt tttcgggctt gcccgggcgg cggaaccggc ccactcttgc cccatcgctt
    25321 ttttggcatg gcaaccagca acgaggcaaa gaccgcgccc ggttgggggc agcgcgcgct
    25381 catcgggcgc catccccggc gcacgctggt ccgcattatc ctgctggcca tcacctcgct
    25441 gatcctcttc cgctacgtgc tcctgcccgt gcgcgtaagc ggcggaagca tgctgccgac
    25501 gtacaaagaa aacggcgtca acttcgtcaa ccgcctggcg tacctgttcc acgaaccccg
    25561 gcgcggggat gtcgtcgccg tccgcctgct ggcgggcgcg caccggctgt acctgaaacg
    25621 gatcgtcggc ctgccgggtg aaaccgtggc cttccaccac ggccggctgt tgatcaacgg
    25681 ccagccgctg gccgaaccct acgtcaagtt cccctgcgac tggaatcaga accccgtccc
    25741 ggtcgggccg gaccaatact acctggtcgg agacaaccgg gacaatcccc gggaaggaca
    25801 cgagcagggc cgcgcggcgc gcgcgctcat catcggcaaa gtgctcctgt aaaatcatga
    25861 aagaatggac gatccgcatc ctgctggtgg cggccctggc cgcgctgggg atctggggct
    25921 ggcgcacgct tcatcccgac ccggaacagg tcatccggcg ccagctccag gagatggcca
    25981 aagccgcatc gttcaccgcg gagcaggggc tggccgccca ggcctggtac gccagttcgc
    26041 tccccgagtt cctggccctg gatattgaag tgacgctgcg cgtgcccgga gtcccggcta
    26101 ccttgagcgg gcgggacgaa gtgatgcagt tgctgacggt ctcccgccgg gtttgccgca
    26161 gccttcgcct ccgcttgccc gacctcaaaa tcagccttgc cccggaccgg acaatggccg
    26221 aggtttatgc cacgggtgaa gccgacgtga cctatgcatc cggcgagaag gcgtctttct
    26281 tgcaggaact gcgcctgcgc ctggtcaagg tcaagcaagg ctggattgtc caacaggtcg
    26341 agacggtaaa aacgcttacc taaggcggcg atttgccggg ggaccgccgg ccctggaaac
    26401 agggggtgcc tttcccgacg gtttccccgg cttcggccgc cgctcccggg ccggctccgc
    26461 cagttgaaaa tccacctggc gctggccggt gtccactttc gccacccgca cctccaccgt
    26521 gtcgcccagc tttagcccgc gccgcgcacc gcgccggggc ggccggcccc gctgggcgtc
    26581 gggcggccca tcgccggccc ccgcgcctgc ggccggcacc agcccgctta gccccagccc
    26641 cggcacatcc acccggcatc cgaagctccg cacctcgatc acccgggcct cgtagtgctc
    26701 ccggtgcgcg gattgaagct gcgccgccag aaacgcgaac agcttcgcct cccggctgtc
    26761 gcgctccgcg tcatcggaat tgcgctcggt cgcggaaaga tgctccgcca tttccgggag
    26821 cggccgggcc gccacgggcc gccccggcgg ccccaacagc gcccggtgaa ccaccaggtc
    26881 cgcgtagcgc cggatcggcg aggtgaagtg cgtgtaatcc ggcttggcca aaccgtaatg
    26941 cccgagcggc tcgaccgcgt agcgcgcgcg cgggagcgac ttcaggaacc cgatcttgag
    27001 cgccgggccg atcggcagcg tcgcgagccg cgccagcagc ttctgcgcct cgcgccggtt
    27061 cgacagccgg ccgcagggaa tatgctggct ccgcacttcc tggcgaaact cctcgagccg
    27121 cgcctcctcc ggcggctcgt ggacgcggta aatcgccggg cccggccggg cccgcagccg
    27181 cccggccgcc gcctcgttgg ccagcagcat gtattcttcg atgagctggt gcgcgctgtc
    27241 gctcgcctcc cgctccaggc gcagcacccg cccgtgcgca tccagccgga tcttgagctc
    27301 cgggaaatcg agctccagcg cccccgcctg gaatcgcgcc cggcggattt tcccggccag
    27361 cgcatgcgcg tcgtgcagca tctgctccag cgggttgcgg ggcggacgcg ccagcagggc
    27421 cagcacctcg ccgtaatcat accggcgctg cgagcggatc accgcggaat aaaaccgcgt
    27481 cgcgcgcacc cgcccgtccg ccgtgagcag aaactccgcg cattgggtca gccgctccac
    27541 ctggggcttg agcgaacaca actcctcgct cagcgccgcg ggcagcatcg gcaccacccg
    27601 gtccaccagg tacgtcgagt tcccccgccg ggccgcctcc tcgtccagcg cgctgccggg
    27661 ccgcacgtaa tgcgacacat cggcgatatg cacccacagc ttccactgtc ccgccccggc
    27721 gcgctgcagg cagatggcat catcaaagtc tttcgcgtcg gacgggtcta tggtgatgac
    27781 cgggtgcgcg cggcaatcca cccgcccggc ccggtccgcc ggcgtcactt cccgccccag
    27841 cgcctgcgcc tccgccagaa ccttccgggg gaaatgcaag ggcaactggt actggcggat
    27901 gaccgaaagc atgtccactc ccgcggcgtc cggcggcccc agcacctcga tgacctcgcc
    27961 cgccggcccg gcctggcgcg gcgtccactc ccccaaacgc acgacaacct tatcgttgac
    28021 ccgcgccggg cgcccggtct ggcgcggcgg cggcacgtga atctcgtggc cgatgcgcgg
    28081 gtcatccgga agcacgtaga aggattgctg gttccgccgc agcgtgccga ctaattgagt
    28141 gcgcgcccgc tccaggacgc gcacgacccg gccggccgcc tcttttgcgc ccgttctccg
    28201 cgcccgcggc ggagcgccct cccgccgcac cagcacccgg tccccatgca gggccggcgc
    28261 cgttgccggg ccgggaatgc gaatgaccgg gatccgggga tcgtccgcct ccaaaaaacc
    28321 cgttccctgc cggttcatcc gaatgcgccc cggcaccagt tccgccgccc gcggcagcgt
    28381 atagcggttg cccggccggt tgcgcatgat ttggccgttc cgctccatgc gcgccagcag
    28441 gcgcgccagg tcttcccggc gatcgcggcg gaaacccagc cgcgcccgca attcggcgac
    28501 attcagcggc gcggagttgg cccggccaag cagggataaa atcttctctt ccatttcagg
    28561 tgattagccc caggtgaggt gtcggcgcgc aaaatgcacg gcggcagccc cgctgtccag
    28621 cttaattgcg gccgcttaac cgacccgctt cgccgccggc gggtgccgcc gcgacacaaa
    28681 caggctgccc gccaggtaaa accgcagcgg cgcgtccgcc gcctttttca agcccacccg
    28741 caccgtggtg atcatggggc ctcgtttccg gcgaaattcc gccaccgccg ggtttcgggc
    28801 cacaaacaac gccgccgccg cgtcgcacaa atcaacgccg tccagccccc ggtcaatagc
    28861 catcgcctgg cacagcttgc cggggccgct ggtcaattcc cgcgcactct cggccgaccg
    28921 gtgctcacgc ataaagtcct ctccaaaagc aacttccacc gcgcgaatca gcaccgcttc
    28981 ggcgaccccc gccggctggc aaaccacgtt ggcgcagaaa taattgccgt aaatcaaata
    29041 cacataccca tgccccggcg cgccgaacat tacgcgattc cgcgccgtca gccctgcggc
    29101 gccatgacag gcgggatccc ccaccaaata cgcctccgtc tcgacaatcg cgcctccaca
    29161 cgccccgttg gctgtgttgc gaatcaacca atgccccagc aacgccggcg cgaccgtccg
    29221 ggccgacggc tcataaaaac tccgcgggag cgcccgaaaa ccattcttca tgccccgcca
    29281 ctctacgacc aaaccgcttc cgcaacaacc caactcctcc cgcaatcgcc caacgaactc
    29341 cccgctcccc caattccgtc cccggacgcc atatccccgc gcccgcccct ttccacgccc
    29401 gttcatctcc gttccgctcc cctcccgata cgccgtgtac aaggggtgta caagggatgc
    29461 tcaccggaag tt
//
LOCUS       DAOPQG010000038        29280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2050_length_29280_cov_17.972934, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000038 DAOPQG010000000
VERSION     DAOPQG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 29280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29280
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2050_length_29280_cov_17.972934"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1113
                     /gene="folE2"
                     /locus_tag="PKI07_06110"
     CDS             205..1113
                     /gene="folE2"
                     /locus_tag="PKI07_06110"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOC50663.1"
                     /translation="MFKPNENGAPRARVRRAAAGAGRRRLPRRPGRGAARGGRPGRAA
                     PAPLHDKQNERDHRELRIDQVGVRGLRFPIQVRDKARLVQNTVATIGMFVDLPPEFKG
                     THMSRFIEVLNAHGNIIHVENLTAILRAMQAKFQAETSRLEIEFPYFMVKQAPVTGME
                     SVMDYQARFDAMARREEIRFLLTVKAGVTTLCPCSKAISDYGAHSQRGEVTVQIRSRK
                     AVWIEDVIALIESSASAELYALLKRQDEKAVTEHAYTHPVFVEDLVRNVALKLNALPE
                     VTWYKVEAENQESIHHHNAYACIEKH"
     gene            complement(1188..4475)
                     /locus_tag="PKI07_06115"
     CDS             complement(1188..4475)
                     /locus_tag="PKI07_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50664.1"
                     /translation="MKRYQKLALVGVVAFLEWAAPAQAARLCERWQQAYSGDDATGTN
                     VMGLWMFRDTRDGSAQGRNATLQGAVIAPGGKFGPCLESFRGYPIEDKRHAAVIPNSP
                     GLTPPGAFTLELWIQPKPEIDGYPEAFLVDKKYVAHTDYQLVLGAADATGQRRLSMRL
                     GFGQDSETYVSSPALYATNEWCHIAFTYDGAGTGSFYRNGAALGGATRPGRAGVAPGP
                     HPLSIGDRIGSLYHGFPGFITQVRICRGVLEFRPASVAYESDRNVFVRMEPAPALAFS
                     VVNRQRAPLNGASVVFALDGAELQRVSLPALASGQSHRIEVPFDTSLRPGEYAVQARL
                     EMGGEMPGAGEETFPVTLTARKPPRMPVVMWGIYGAANVIKELPRLKEIGFTHCLGFG
                     ADMGAIYDAGKPAAPDKPENVAAVKRMLNTALANDLGVLASLSPGRWAAESRTELRRI
                     GKNGKPPDGKPSLCAAAPELRPFCFNVGASVAQAYGKFPAWQAALLHTEVRDGADVCY
                     HAHDRAACRQATGQDYPVETPGKRGVDYTQLKDFPADRVIPDDHPLLRFLAWYWQEGD
                     GWNRLNTELHRGLKSTGRPDPWTFHDPAVRVASVYGSGGQVDVISQWTYSYPDPIRIG
                     LATDELLAMARGAAHPQRVMKMTQIIWYRSQTAPLAKGDEVRKGARSPWEDTEPDAAF
                     LTIAPMHLREAFWTKLARPVQGIMYHGWGSLVPDTGESAYRYTHPQTRHELRRLIKAV
                     VEPLGPTLLQVPDRPADVAFLESFASQMFARRGTYGWGNGWAGDAYQALLWARLQPEI
                     VFDETVARGGLDRFRILVMMDCDVLTARVVSRVRAFQQAGGIVVADERLCPAITADVV
                     IPSYTRTRKADQDRTALMAKAAELRAALGDRYQPYSDSSTPDVVTRCRAYGSTDYLFA
                     VNDRREFGDYVGQHGLVMENGLPTEATLSLARPRGHVYDLAAGRELEPIPGRSPLQIR
                     REFGPGEGCVLMVTAAAIAGVRIEAPRQAVAGQSIPCGISVVDADGQPLNAVVPLQVE
                     ISDPAGAEAEWSGFHGARDGRLQLRLAIAPNDRPGLWRIHARELASGRAAAAYFRVEP
                     PAKPPPR"
     gene            5047..5511
                     /locus_tag="PKI07_06120"
     CDS             5047..5511
                     /locus_tag="PKI07_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOC50665.1"
                     /translation="MIAVKPQWAKYYVHLLELRDQLTRQRDGRARESAEQIASYSLHM
                     ADSGTDNFDRDFALSLLSADQDALYEIDQALKRIEKNTYGICELTGKPIPPARLEAIP
                     WTRFTVQAQAQLEREGALRQRRLASLGSVDAAGAAEPEPEDEDAEEKSKEKE"
     gene            5514..5681
                     /gene="rpmG"
                     /locus_tag="PKI07_06125"
     CDS             5514..5681
                     /gene="rpmG"
                     /locus_tag="PKI07_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOC50666.1"
                     /translation="MPREIITLECTEARKEGKSPSRYTTTRNKKLKPEKMQIKKYNPA
                     LRRHTLHREIK"
     gene            5691..5957
                     /gene="rpsR"
                     /locus_tag="PKI07_06130"
     CDS             5691..5957
                     /gene="rpsR"
                     /locus_tag="PKI07_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOC50667.1"
                     /translation="MPRKTRTDKPDKRGGRKRSAYGETRAPRPKPKIDFTLDTLDYKN
                     VALLRQFVTDQGRILPRKYTGLPAHYQRRLNCAIKRARQMLLMK"
     gene            complement(6017..6181)
                     /locus_tag="PKI07_06135"
     CDS             complement(6017..6181)
                     /locus_tag="PKI07_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50668.1"
                     /translation="MTKSLEHIGEAVQNPKHSFRKSDSRPKKSLKHRYERRKIKEYLQ
                     LGEWTAREAT"
     gene            complement(6577..8130)
                     /locus_tag="PKI07_06140"
     CDS             complement(6577..8130)
                     /locus_tag="PKI07_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020152137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOC50669.1"
                     /translation="MKPLLPAALSAWLLPLTLALGQSSPARPNILFIMSDDHAAHSIS
                     TYGSRVNQTPHMDRLAREGMRFANCFAVNSICSPSRATILTGKYSHLNGVPTFNRFDG
                     SQPTVAKYLQKAGYYTGMIGKWHLGSEPTGFDHWIVLPGQGAYFDPAFLTPDGRHVRK
                     GYVTDLITDLALDFLRNRPPDKPFFLMCHHKAPHRPWEPDEKHRAQFAGKFIPEPATL
                     RDDYTTRTDAIRECRQKVLEDLTRRDLKLEPPAGLSVAERNKWLDVAPMEIETVINGQ
                     TRKLAGEELRAWKYQRYLQDYLACIQSVDDSVGRLLAWLDQNGAATNTVVIYTSDQGF
                     FLGDHGLYDKRFMYEESIRMPFLVRWPGVIKPGSTQAAMAINPDFAPTFMELAGQRPP
                     PDMQGRSLVPLFKGSRPADWRTSWYYRYYHDPGNHNTRAHYGLRTETQKLICFWKTDQ
                     WEMYDLVQDPAELHNLYYDPAHKKQAERLKAELYRLKKEVKDDDQYAVQQPPHDVSGV
                     PAAKPKPKP"
     gene            complement(8141..8914)
                     /locus_tag="PKI07_06145"
     CDS             complement(8141..8914)
                     /locus_tag="PKI07_06145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOC50670.1"
                     /translation="MQLEWPWCRPPPAAPASAWVAVGSRSVRLWFVRHRRARRYVLRL
                     RADGAVRVTVPRGGSVAEAQRFAARSAPWIERQLLRQARAPQPPARWPAGTEFLFRGE
                     RVRLEAARTDAGNVIRFGTEAVPVAGMDGDLRPAVEQHLRRLAALELPARVRELAARH
                     HLSVRRVTIRSQRSRWGSCSPRGTISLNWRLVQTPPWVRDYLVLHELAHLREMNHSRR
                     FWAEVARLCPNYREAERWLKEHADLLTPQIQGCPPPGGG"
     gene            9038..9367
                     /locus_tag="PKI07_06150"
     CDS             9038..9367
                     /locus_tag="PKI07_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HOC50671.1"
                     /translation="MHWIYLAVAIVSEVIGTAALKAAQGFTRGVPTLLVVTGYGISFY
                     FLSLVLRTIPLGVSYAIWSGVGIALVTLAGWFIYDQRLDLPTFLGIGLIIAGVIVINV
                     FGQSAAA"
     gene            complement(9399..9635)
                     /locus_tag="PKI07_06155"
     CDS             complement(9399..9635)
                     /locus_tag="PKI07_06155"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOC50672.1"
                     /translation="MTPEGEVGVQTIAGGRYAVFLHRGPYENLLGAYQHILGEWLPGS
                     GEELRDVPCFERYLNSPGHTRPENLKTEIYLPLK"
     gene            9849..11600
                     /locus_tag="PKI07_06160"
     CDS             9849..11600
                     /locus_tag="PKI07_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOC50673.1"
                     /translation="MAFLSLKQFLAQVGLATPEQFDEWNKAWKVAAASGSQEPLLAFI
                     CRERGLAEDVFLQQLAHKLQWPYLDLPRVTIPAEVRNRISTKVAFQHTVLPTQAGEGG
                     LQVVVSNPFDAAMLNAVRFDARGPVQFALAPKAEIEKALKKYYGVGAETLDEMAEDEP
                     LELLVGEQKEITEGDQEASVIKFVNQVIWEAFKDRSTDIHFEPAEDELRIRYRIDGIL
                     HQTPMPPQLKRYQAALISRIKVMSGMNIAEKRLPQDGRINVRIKGEEIDIRVSTVPTV
                     YGESVSLRLLTRGKIFLSLDKLGFSPLEEKAIREIIVKPHGIFLVTGPTGSGKSTSLY
                     AFLSTINSVHKRIITIEEPVEYELKGINQIAVRPEIGLTFAMGLRHILRQDPNVIMVG
                     EIRDLETAEIAIRAALTGHLVFSTLHTNDAPSAFTRLIDMGIEPFLVASSVESVMAQR
                     LVRTICPHCKQEQKVEKDYLRRIGFPEHEIETAKFWHGVGCEDCRQLGYQGRMGIYEL
                     LILNESLRPLILNRAPASTIAQQAIEFGMRTLRTDGWNKVRAGQTTIEEVLRVTQIEE
                     HLDALAEDRKVAAGIKR"
     gene            11597..13576
                     /locus_tag="PKI07_06165"
     CDS             11597..13576
                     /locus_tag="PKI07_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50674.1"
                     /translation="MKTWRTITGGGGCRFSFCALFGRAGGRLIAAALLGFGLAGCDPA
                     PHSAAPPAGTNAAAPRPAAGSPATALRTPGARTNAPAVAAPATARVQTPGAPLPPAPK
                     GPVPPAPARPGTGTNALASAVPPGVGTNAASATGAGSGIADQLRALTASRVFFPVLGI
                     IVLSLCLVAMWVWKRVKAKEPKPARAEPAAAAKTASPAARKKPGKSSVHSCNVLQVDA
                     QARQLWQFDAKGRNFVLNREHTVPAGKALPASLVAKDWRSLFQPRLNVVWLPPERVFL
                     RVVQLPVSDLDETLAMVELQLERLSPFPVAQIVWSIQILPPAEGNLQTVIVLIASRNE
                     VEEFLGALEGQGYLADRLELPLLDQLQATAITADGAWVYPEPSGSRNTALVAWWYGGV
                     LQNLGLLTLPPSGRPESLREQLLQMAWAGELEGWLTTPPTWHLVADGPAAADWEPPLR
                     TGLAQPVELVEPVTVAGLAALTATRATRAGAQANLLPEEYATRYRQQFVDRLWMRGLG
                     AIVALYLAGVLIYFARLQVELYRTHAVEQQAAERGPIFTNALQLKAKLQVLKDRQDLK
                     FAALDCWKAVAELLPDGVTLESYNFSEGKKLTLSGTAPADQTKLLTAFHDDIRKVAPN
                     GQLLFNPNTPPLTWERRGETVSWHCVLELRRTEAL"
     gene            13573..14193
                     /locus_tag="PKI07_06170"
     CDS             13573..14193
                     /locus_tag="PKI07_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50675.1"
                     /translation="MTSYLDRLNLRPFEKRLVVVVGIVLFVVLNMWFVVPHFSDLSDA
                     QRRRVEAQKKLERWQAEIDQAQKYQAGINTFARQGLDVPVEDQQNQFVRVIQDQQSQS
                     GVGIQSLGRTSTQTNQFFLELTQLITVQSQEAQLVDFLYSLGAGDSLIRVRDLSLKPD
                     PPRQQLSGMVKLVASYQKNPPRKTAPDAKTPPASAKMATPSPTPNP"
     gene            14706..16514
                     /locus_tag="PKI07_06175"
     CDS             14706..16514
                     /locus_tag="PKI07_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOC50676.1"
                     /translation="MIDFREADLKQVLDIYSDMVQRTVLRPATLPAPTITLSTRGHLT
                     LGEGIQALEAVLALNGIVMVNVGEKFVKALPEAQGISSGARFDTNSAAQMPELGQYVT
                     HIVQLKYVRPSEMVAVLTPFGKIPNAILPIDSSQILVLRDYTENIKRMLELIEKVDVV
                     IPSEYVSEVIPIKYAQAVEIANALSSLSSGGGGATVGSTGTGTSRSSRSTGINRPGSM
                     TGMSGYPGQQPMTGMATPPGRAGTPAAGAGGSTFTQRLQNIINRASQEGEIQVLGQTK
                     IISDERTNSLLVYATREDMKVIKEIVGKLDVVLAQVLIEAAIIEVTLSDSRDLGFSYL
                     QRPQHTGKWTGVGAINYNNMLQPGGFAGGGGTNNPVGQGFNYLMSWNQDLDATVTALA
                     SDSRARILQRPRIQTSHNEPASLFVGESRPYPTSSYYGGGAYGGYSSINQLQIGVTLE
                     VTPLINPDGLVVMEIQQQIDSFQGNVTIQNVGDVPITSSKIASTKVSVRDHDTIIVGG
                     LVETQKNKNASGVPYLKDVPLLGYLFRSTHAAETRKELLVLIRPTVLPTPEVAALAAT
                     AEKNKLPGVRETEHELRTEEIRRLKAADKAEKGGRP"
     gene            16632..20174
                     /locus_tag="PKI07_06180"
     CDS             16632..20174
                     /locus_tag="PKI07_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOC50677.1"
                     /translation="MLVLLGALLAGGLPAGGAVPPEIDPGALEEDDGTLDFGDPTNNG
                     NLVSDCSQSQIQEFLAAVREGGTVELTDCPRLVLTNAVAITTDVTLRGDEDGKTTITG
                     NNLSRMFTVYPRGCLTLENLTLQGGLSRYGGAIYNMAGGRVVLNNCRIVGNQAVGRNG
                     VNGRNGDNDPNYGKKGKSASSGQSGAGGGIYNLGNLEATDCQFTDNRAKGGHGGDGGN
                     GGEGRYQGGNGGDGGDGALAFGGAIYSEGRLWLTNCQFHTNAATGGNGGRGGSPGTSV
                     FAGYDGTGGAGAEGSGAAIYSAGGMVLSNCAFFGNTARGGNSAAAGEAGGGYGVNGAD
                     GGYSYGGAVCLLGDGRLIHCDFANNTAAGGMGGDGGPGRYVAGNGGNGGKAVGGCLYN
                     YGGARLVDCFLTNCAAIGGSNGVGGSAPFAGKDGRPRGADDKSMRVRESETIRAVSLP
                     EGAVPAATGVATVLLAEHPVAAESARNTPPQAGLSPADSKAANLPPGLPAHLVGTNLL
                     VKPRASTAETNGSPPALPAAGNRSPSSPGGPFTPVAGPAVPPGPGSGLPSRGSAASLE
                     APLPEGMIDFREADLKQVLDIYSDMVQRTVLRPATLPAPTITLSTRGHLTLGEGIQAL
                     EAVLALNGIVMVNVGEKFVKALPEAQGISSGARFDTNSAAQMPELGQYVTHIVQLKYV
                     RPSEMVAVLTPFGKIPNAILPIDSSQILVLRDYTENIKRMLELIEKVDVVIPSEYVSE
                     VIPIKYAQAVEIANALSSLSSGGGGATVGSTGTGTSRSSRSTGINRPGSMTGMSGYPG
                     QQPMTGMATPPGRAGTPAAGAGGSTFTQRLQNIINRASQEGEIQVLGQTKIISDERTN
                     SLLVYATREDMKVIKEIVGKLDVVLAQVLIEAAIIEVTLSDSRDLGFSYLQRPQHTGK
                     WTGVGAINYNNMLQPGGFAGGGGTNNPVGQGFNYLMSWNQDLDATVTALASDSRARIL
                     QRPRIQTSHNEPASLFVGESRPYPTSSYYGGGAYGGYSSINQLQIGVTLEVTPLINPD
                     GLVVMEIQQQIDSFQGNVTIQNVGDVPITSSKIASTKVSVRDHDTIIVGGLVETQKNK
                     NASGVPYLKDVPLLGYLFRSTHAAETRKELLVLIRPTVLPTPEVAALAATAEKNKLPG
                     VRETEHELRTEEIRRLKAADKAEKNKPASSRLAP"
     gene            20337..21743
                     /locus_tag="PKI07_06185"
     CDS             20337..21743
                     /locus_tag="PKI07_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC50678.1"
                     /translation="MKNFFSGRFRPGAMRGGGLGAALLLLLAVSAWSADTFNWDTNRN
                     RVSADIKATQLRPLLEQIAAVTGWHILLEPDTTCDVSAKFENLPPGEALRLLFRDLNF
                     ALVPDTNASPRLFVFRTALRNATQSIPPARAAESASQGKLIPNELILRLKPGAKIDEL
                     ARLLGAKVTGRIDSLNAYRLQFPDAAAAEAARQQLAAHPEVLSVDSNYSIDRPPGPAG
                     TSAANLLPPPRLQLKPPPNDGRIVVGLVDTAVQPLGNDLDAFLLKPLSVAGEAQLDPH
                     LPSHGTSMAETILHSLAAMTKDGSTVRILPVDVYGPNPTTSTFDLARGIAEAVNGGAN
                     LISLSLGSEGESPFLHNLIQDAHARGILFIGAAGNSPVTTPFYPAAYPEVMAVTAIDQ
                     GQIASYANRGSFVSFGAPGTSIIYFNNQPYYVTGTSAAAAFLSGIAAGYMDSTRSDAR
                     QMQTFLQSNFGVKITPSR"
     gene            21826..22710
                     /locus_tag="PKI07_06190"
     CDS             21826..22710
                     /locus_tag="PKI07_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOC50679.1"
                     /translation="MRWKKVTLIGVGLLGGSLGLALRERGRAKSVVGFVRRAASVAEC
                     ERLGAVDLATRDLARAVAKAELVVLCTPLARMRPLVETMLPLLSPGAVVTDVGSVKGS
                     VVRDLEALVARAGAHFVGSHPMAGSERMGVAAARADLFAGAVCVVTPTRQSNPAAVRQ
                     VEQLWQAVGARRLRLSPAAHDQMVSRSSHLPHVVAAQLTNLVLGPKRPRAQGLLCANG
                     FRDSTRIAASSPEMWRDIALANRENLLRALAGFASGLRDFQRALKAGDARAVSKFFAQ
                     ARKRRLAWARGAGARPAE"
     gene            22737..24005
                     /locus_tag="PKI07_06195"
     CDS             22737..24005
                     /locus_tag="PKI07_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50680.1"
                     /translation="MALPELIEIVPLDRPVRAEITVPGSKSITNRALILAALGPGETT
                     LPGALWSEDTQVMAAALRELGFAVNVAPDPREPGNRVITVGGQGGRVPRAGTVEHPRE
                     LFVGNAGTAARFLAALVCLGRGVYRLRGVPRMQERPQAALFRALRELGYRVESPNDRL
                     PAVIHGAGARAAHCEVSILESSQFASALLLCAAAGKWRVTVSGENAEESSYVALTRKL
                     AAAFPRSGGQFPIEPDASSASYFLGAGHLIPGSAVTLRAPPATDWQVDAAFPRFLPLP
                     ASLSRRDELGDSIMTAIVLAPLARQAVRFTDLGRLRVQECERVQALRTELTRCGAAVA
                     EAGDTLTVFPSPLHGAEIETYQDHRMAMCFAILGLKIPGIRLKNPACVKKTFPNFFQK
                     LAAPPPHGLGAELREATSGRVLRPDELLAE"
     gene            24031..24711
                     /gene="cmk"
                     /locus_tag="PKI07_06200"
     CDS             24031..24711
                     /gene="cmk"
                     /locus_tag="PKI07_06200"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOC50681.1"
                     /translation="MPQPRKQAPVVIAIDGTSASGKSTNSRLVAQALGFIHVDTGAMY
                     RTLAWYCLRHQIDVRNARAVAAACRRWKATLQCADGQVYLAVDGYYPATEIRSLEVSA
                     VVSHVAAVPKVREWMKKKQRECLQFGNLVMEGRDIGTNVFPETDFKYYLDAKLEERAR
                     RRAADGLQENLAQRDQRDSQRAVAPLMIALGAKVINNSQMTPEQTSGTILADIRQRLA
                     AAARAPDR"
     gene            24718..25407
                     /locus_tag="PKI07_06205"
     CDS             24718..25407
                     /locus_tag="PKI07_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOC50682.1"
                     /translation="MNFWYFLGWCAYRTLFRFYFRWRVYNAGRVPRQGGVILASNHAS
                     YLDPPLVGAGLRRGIHYLARENLFRFPVMGWVLRQWQVVPVDRDGGGAKGLKAILDRL
                     LAGGAIILFPEGTRTRDGRLQPARSGIGLTVIKSTAPVVPVRVFGTFAAYGRHLRLPR
                     PFRVAVKYGPPMDFAALRAEAQLCSKARLKEIYQQVADDIMAAIAQLGPYEDLTRFPA
                     KTNSPASRPRA"
     gene            complement(25370..26389)
                     /gene="ruvB"
                     /locus_tag="PKI07_06210"
     CDS             complement(25370..26389)
                     /gene="ruvB"
                     /locus_tag="PKI07_06210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOC50683.1"
                     /translation="MAEHRISEALAQPDRALEMTLRPSLFAEFTGQLRVKERLEITVA
                     AAQQRREAIDHILFSGPPGLGKTTLAHILAKAMGANLKSTSGPTIEKAGDLAGLLTNL
                     EEGDVLFIDEIHRLQKTIEEYLYPAMEDFKLDIIIDQGPNARSVRLNLPRFTLIGATT
                     RSGLLTAPLLTRFPIRERLDYYHAEDLQQIVVRSARLLNVEIEKEGAREIARRSRGTP
                     RIANNLLRRVRDYAQVRHTGHITLEVADKALAMLEIDENGLDEMDKRILETVIVKFGG
                     GPVGLSSLAVAVGEEPDTLEEVYEPYLIMEGYLKRTPQGRVATELSYQKLGVKPAGGK
                     QASLF"
     gene            complement(26453..28459)
                     /locus_tag="PKI07_06215"
     CDS             complement(26453..28459)
                     /locus_tag="PKI07_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HOC50684.1"
                     /translation="MSLVCNDTTAAADAPGAGAADRDLDLPIKGFSGTPDEIERQWFE
                     KIYTGRGDVQRQLTLRAVLMGGGLGMFMSVSNLYTTLKLGWAFGVAITACVLSFVIWN
                     GLRALSGGRLSPMSILENNCMQSTASAAGYSTGGTIGTAFGALLLLEGMHRPWYVVGA
                     FALFAAALGVFLAIPMKRQMINQEQLKFPSGIAAAETLRSLYSRGREALQKAYALIGA
                     LAAGGLVGGLRSYGTLVEQFRGTGRPQAWLERLHQVLSLPEALAAPRWLSPIPHGQMA
                     GLVFEPSVLLIGAGMITGLRVSLSMLLGATLLYFVVAPRVLLRDLTHAGVAGYVPSFT
                     LLPDGSFNPIRWALWGGTAIMVFSSLATVALEWRTLARAFSLFRKKERTAHSAAMDAI
                     EVPVSWLVAGLVPITLGMVIIQYLAFHISVPLGLIAVALAFVVSLVCCRATGETDTTP
                     VGAMGKVTQLLYAVLPGARGVASINLMAAGATAAAGGAAADLLTDLKSGYILGANPRK
                     QFLAQFVGIFFGTLAIVPAWYALVPNPKALEAFNPPATFMWKAVADLLTQGVHMLPVT
                     ALWAIVGGALIGVLLPLAAKLAPRARPYLPSAMGLGLAWVMPFANSLSFALGAVLVLL
                     WSRARRRAADLYYVPVASGLIAGESLVAALIAIACTGLGLLAVK"
     gene            complement(28865..>29280)
                     /locus_tag="PKI07_06220"
     CDS             complement(28865..>29280)
                     /locus_tag="PKI07_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50685.1"
                     /translation="NGQSDITDLYTARFGRPAPGQKVFIAVVQTRNGWKGQSRVHNAI
                     VPPPPLLKSQQAAETARVKKSKPAAAPTSTAAHKTVPSPSFQAMYNGSTSEARQEHME
                     NKHESPLSLLCTPVVHSLMGALGWLGRVGRVWARA"
BASE COUNT         5067 a         9083 c        10056 g         5074 t
ORIGIN      
        1 atcggcttgt cggcagaagc aaacccgctt caaacgttgc gggcagttct ggaccccaac
       61 cgggctccgc aacctctgcg cgctcgatga agcccgccgc aaccacccct gggacgagct
      121 ttgggcctcc aactaaggac agtgttcaga tgcaccccgg gcgaagcatt ttacatcgcc
      181 gccggcatcg gttagattgg gcgcatgttc aagccaaatg agaatggcgc cccgcgcgcg
      241 cgcgtccgcc gcgccgccgc cggggccggg cgccgccgcc tgccgcgccg tccggggcgc
      301 ggggccgccc ggggcgggcg tccgggccgg gccgcgccgg ccccgctgca cgacaagcag
      361 aacgagcggg atcaccggga gctgcgcatt gaccaggtgg gcgtgcgggg gctgcggttt
      421 cccatccagg tgcgcgacaa ggcgcggctg gtgcagaaca ccgtcgccac gatcggcatg
      481 tttgtggatt tgcccccgga gttcaagggc acccacatga gccggtttat cgaggtgctc
      541 aacgcgcatg gcaatatcat ccacgtggag aacctcaccg ccatcctgcg cgccatgcag
      601 gcgaagttcc aggcggagac ctcccggctg gaaatcgagt tcccctattt catggtgaag
      661 caggcgccgg tgaccggcat ggaaagcgtc atggactacc aggcgcggtt cgacgccatg
      721 gcgcggcggg aggaaatccg gtttttgctg acggtcaagg cgggcgtgac caccctgtgc
      781 ccgtgctcga aggcgatttc ggactacggg gcccacagcc agcgcggcga ggtgacggtg
      841 cagatccggt cgcggaaggc cgtgtggatc gaggatgtga ttgccctgat cgagagctcc
      901 gccagcgccg agttgtatgc cttgctcaag cggcaggacg agaaggccgt caccgagcat
      961 gcctacacgc acccggtgtt tgtggaggac ctggtgcgca acgtcgcgct caagctcaac
     1021 gcgcttcccg aggtgacgtg gtacaaggtg gaggcggaaa accaggaaag catccaccac
     1081 cacaacgcct acgcctgcat cgagaagcat tgatcgcccg gccggggcgg ggcgggcggg
     1141 gccgcgcggg gccgggttgg ccatgccggc ggcgcggcgc ggtttgttca acgcggcgga
     1201 ggtttcgccg ggggttcgac gcgaaagtac gcggcggcgg cgcggccgga agccagttcc
     1261 cgcgcgtgaa tccgccacag cccggggcga tcattggggg cgatggcgag gcgcaactgc
     1321 aaccgcccgt cgcgggcgcc gtggaagccg ctccactcgg cttcggctcc ggcgggatcg
     1381 ctaatttcca cttgcagcgg caccacggca ttgaggggtt ggccgtccgc atccacgacc
     1441 gatatgccgc acgggattga ctgtccggcc accgcctgcc ggggggcttc aatgcgcacc
     1501 ccggcgatcg cggcggcggt gaccatcagc acgcagccct ccccggggcc gaactcgcgg
     1561 cggatttgca gggggctccg gccggggatg ggttcaagct cgcgtccggc cgccaaatca
     1621 taaacgtgcc cgcggggccg ggccagcgag agggtggcct ccgtgggcag gccgttttcc
     1681 atcaccaggc catgctggcc gacgtagtcg ccaaactcgc gccggtcgtt tacggcgaac
     1741 aggtagtccg tgctgccata ggcgcggcag cgggtcacga cgtccggggt ggaggagtcg
     1801 gaataaggtt gataacgatc tccgagcgcc gcccgcagct cggccgcctt ggccatgagc
     1861 gcggtgcggt cttgatcggc tttccgcgtg cgggtgtagc ttgggatgac cacgtccgcc
     1921 gtgatcgccg ggcataaccg ctcgtccgca accacgattc ccccggcttg ttgaaacgcc
     1981 ctcacccgcg atacgacgcg ggcagtcagc acgtcgcaat ccatcatcac caggatgcgg
     2041 aaacgatcaa gcccgccgcg ggccacggtt tcgtcgaaca cgatttccgg ctgaagccgc
     2101 gcccacagga gggcctggta agcgtccccg gcccagccgt ttccccagcc gtaagtgccg
     2161 cgccgcgcga acatctggga ggcgaaactt tccaggaagg ccacgtcggc cgggcggtcc
     2221 ggcacctgca ggagcgtggg gccgagcggc tcgaccaccg ctttgatcaa ccggcgcagt
     2281 tcgtggcggg tttgcggatg cgtgtagcga taagcgcttt cgccggtatc cggcaccagt
     2341 gatccccagc cgtgatacat gatgccttgc accgggcgcg cgagcttggt ccagaacgcc
     2401 tcgcgcaggt gcatcggcgc gatcgtgaga aacgcggcgt cgggctccgt gtcttcccag
     2461 ggggagcggg ctcctttccg aacttcgtct cctttggcca ggggcgcggt ttgcgagcga
     2521 taccagatga tctgcgtcat cttcatgacc cgttgcggat gagcggcgcc gcgggccatg
     2581 gccagcaact catccgtcgc cagcccgatg cggatcgggt ccgggtagga ataggtccat
     2641 tgggagatga catccacctg gccgccgctg ccatacacgc tggccacgcg cacggccggg
     2701 tcatgaaacg tccacggatc cgggcggccg gtggatttca gcccccggtg cagctcggtg
     2761 ttcagccggt tccagccgtc gccctcctgc caataccagg ccaggaaacg caagagcggg
     2821 tggtcatccg gaatcacgcg gtcggccgga aaatctttga gttgggtgta atccacgccg
     2881 cgtttgccgg gagtttcgac cggataatcc tgtccggtgg cctggcggca agccgcgcgg
     2941 tcatgcgcgt gataacagac atccgccccg tcccgaacct cggtgtgcag gagcgcggct
     3001 tgccaggcgg ggaacttccc gtaggcctgg gcgacggaag cgccgacgtt gaaacagaaa
     3061 gggcgcagtt ccggcgccgc cgcgcacagc gacggtttcc cgtccggggg ttttccgttt
     3121 ttgccgattc gccggagttc ggtccgggac tccgccgccc agcgcccggg cgataaggag
     3181 gcaagcacgc ccaggtcatt ggccaatgcg gtgttgagca tgcgcttgac ggccgccacg
     3241 ttttccggtt tgtccggagc ggcgggcttg ccggcgtcat agattgcgcc catgtccgct
     3301 ccgaacccga gacagtgcgt gaacccgatt tccttcaggc gcggcagttc cttgatcacg
     3361 tttgccgcgc cgtaaatccc ccacatgacg acgggcatgc gcggcggttt ccgggccgtc
     3421 agggtgaccg ggaacgtttc ctcgcccgcg ccgggcattt cgccgcccat ttcaagccgc
     3481 gcctgcaccg cgtattcacc ggggcggagc gaagtgtcga agggcacctc gatccgatgc
     3541 gactggcccg aggccagggc gggcaaggac acgcgctgca attccgcgcc gtccagcgcg
     3601 aatacgacgg aggctccgtt caggggggcg cgctgccggt tcaccacgga gaacgcgagc
     3661 gccggcgccg gctccatgcg cacgaacacg ttgcggtccg attcatacgc caccgaggcg
     3721 gggcggaact ccagcacgcc ccggcaaatc cgcacctgcg taataaagcc cggaaaacca
     3781 tgatacaggc tgccgatgcg gtcgccgata gaaagcgggt gcggcccggg agccaccccg
     3841 gcccggcccg gccgggtcgc tccgcccagc gccgcgccat tccgataaaa gctccccgtt
     3901 cccgcgccgt cgtacgtgaa ggcgatgtgg caccactcgt tcgtagcgta gagggccggg
     3961 gaggagacgt aggtctccga gtcctggcca aaaccgagcc gcatgcttag ccggcgttgg
     4021 ccggtcgcgt ccgcggcgcc cagcaccagt tggtaatccg tatgggccac gtatttctta
     4081 tccaccagga aggcttcggg atagccgtca atttccggct tgggttgaat ccacagttcc
     4141 agcgtgaacg ccccgggggg tgtgagcccg ggagaattgg gaatcacggc tgcgtgccgt
     4201 ttgtcctcaa tcgggtagcc gcgaaaggac tcgaggcacg gcccaaactt gccgccgggc
     4261 gcaatgacgg ctccctgcaa agtggcgttc cgcccttggg ccgatccgtc ccgggtatcc
     4321 cggaacatcc acaggcccat tacgtttgtt ccggtggcat cgtccccgga atacgcttgt
     4381 tgccagcgct cgcacagtcg cgcggcttgc gccggcgcgg cccattccag gaacgccacc
     4441 acaccaacca gggcaagttt ctgatatcgt ttcattgggc tgccgagttg tctcacgggt
     4501 aatgttcccc ggggcggggg gccgccaggt cttggttgac tcacgatcca ggttacccgg
     4561 gacagtgaag aatatcttca gcctggcctc aatccttttc tccagggcaa aggggtccgg
     4621 cgcggcgtgt ggggcgcgca gccgggcctg ggttggtttg gggatttgag ggctttcccg
     4681 cagtcgttga cgggagaggc gcggtttctt ttcatcgggc ttgatggtct tgcttcgcaa
     4741 attcttgaag attgttgtgg cgtggcggcc cggttctgct atattccaca gctctcgaag
     4801 ggacgcccgg cgttccgagc gaggcggtca ggaaccgcag tttgagtgaa ccgagcagtt
     4861 tccggtgtga gtacgaaaaa gaaaaaagcg attaagaagg ccaagccgct ccccaaagcg
     4921 cgccccaagc cgcgtcccaa ggcgcgtccc aaggccgcgc cccgggcgcg ttcgaagccg
     4981 catggcgcgg ccacggccgc ggccattttg gggcggcccg cgtcggccgg tgtgaaaaag
     5041 ggcggcatga tcgcggtcaa gccccagtgg gcgaaatact acgttcacct gctggaattg
     5101 cgcgaccagt tgacccgcca gcgcgatggc cgggccaggg aatcggccga gcagatcgcc
     5161 agttacagtc tgcacatggc ggattccggc acggataact ttgaccgtga ttttgcgttg
     5221 agcctgctgt ccgccgacca ggacgcgctt tacgagattg accaggcgct caagcgcatt
     5281 gaaaagaaca cgtacggcat ctgcgagctg acgggcaaac ccattccccc ggcgcgcctg
     5341 gaggccattc cctggacgcg ctttaccgtg caggctcagg cccagctcga acgggagggc
     5401 gccttgcggc agcgccgcct ggcctcgctg ggctctgtgg atgccgccgg cgcggcggag
     5461 cccgagcccg aagacgaaga cgccgaagag aaatccaaag aaaaggaata atcatgcccc
     5521 gagaaatcat tacgctggaa tgcaccgagg cgcgcaagga gggcaaatcc ccttcccgct
     5581 ataccacgac tcggaacaag aagctcaagc ccgaaaagat gcaaatcaag aaatacaacc
     5641 cggcattgcg ccggcacacg cttcaccgcg aaatcaagta gccacgattt atgcctcgca
     5701 agactcgcac cgataaaccg gacaaacgcg gcgggcgcaa gcgctccgcc tacggggaaa
     5761 cccgcgcccc gcggcccaag cccaagattg atttcacgct cgatacgctg gattacaaga
     5821 atgttgcgct gttgcgccag tttgtgacgg accagggacg cattttgccc cgcaaatata
     5881 ccgggttgcc ggcgcattac cagcggcggc tcaattgcgc catcaagcgc gcgcggcaga
     5941 tgctgctcat gaagtaggga aaaaaaagcc acgccggcgg cgtggcagat tcatttatgg
     6001 ggagatcccg gggtcgctag gttgcttccc gggcagtcca ctctcccaac tgcaggtatt
     6061 ccttgatctt ccggcgttcg tagcgatgtt tgagggattt cttgggtcgg ctatccgact
     6121 tgcggaaaga gtgtttgggg ttttggactg cttcaccgat gtgttccagc gatttagtca
     6181 tagcattgtt tattgattga ggtttagcca agccagaagc gtgccattcc cccctgcgcg
     6241 atttgtccga tggtaggtta aacaggacat aggccgctgc tgacgctttc aatataagac
     6301 atcttccgtc ctttttcatt caaatattgt ttccctcccc cgcgggcctt tccggcccgg
     6361 gccggaccct cccgccgcgg ttccgtccga aaaacagcca cccggtgcgc ttgccccggc
     6421 cggaagcagc ccgggtgggc tgcgggggga cgcgcggctg gcaggtgaaa aaaaaggggg
     6481 ctagccggtc tctgggacca gcgcgccgca tccgggattt cggggccgcc ctggcttcag
     6541 cgtgtccgct ggttgagcgg gtcgccgggg ggcggttcag ggtttgggct tgggtttggc
     6601 cgccgggacg ccggacacgt cgtgcggcgg ctgttgaacg gcgtattggt cgtcgtcctt
     6661 gacctccttt ttgaggcggt aaagctcggc tttgaggcgt tcggcctgtt ttttgtgggc
     6721 cgggtcgtag tagagattat gcagctcggc cgggtcctgg acgaggtcgt acatttccca
     6781 ctgatccgtc ttccagaagc agatcagctt ttgggtttcg gtgcgcaggc cgtagtgggc
     6841 ccgggtgttg tggttgccgg ggtcgtggta gtagcgatag taccagctcg tgcgccagtc
     6901 ggcgggccgt gatcccttga acagcggcac caggctgcgg ccctgcatat ccggcggcgg
     6961 gcgttgcccg gctaattcca taaaagtggg ggcaaagtcg gggttgattg ccatagccgc
     7021 ctgggttgac ccgggcttga ttacccccgg ccagcggacc aggaagggca tccggatgga
     7081 ttcctcgtac atgaaccgct tgtcgtagag gccgtggtcg ccgaggaaga atccctggtc
     7141 gctggtgtag attaccacgg tattggtggc cgcgccgttc tggtccagcc acgcaagcag
     7201 ccggcccacg ctgtcgtcca ccgactggat gcaggccagg tagtcctgca ggtagcgctg
     7261 gtatttccag gcgcggagtt cctcgccggc cagtttccgg gtctggccgt tgatgaccgt
     7321 ctcgatttcc atcggcgcga cgtccagcca tttgttgcgc tccgccacgc tcaggccggc
     7381 ggggggttcg agcttgagat cgcggcgcgt cagatcttcc aggaccttct ggcggcactc
     7441 gcggatggcg tccgtgcggg tggtgtagtc atcgcgcagc gtcgcgggtt cggggatgaa
     7501 cttgccggcg aactgcgcgc ggtgtttttc atccggttcc caggggcggt ggggggcttt
     7561 atggtggcac atcaggaaga acggcttgtc cgggggccgg ttccggagga agtccagcgc
     7621 caggtcggtg atgaggtcgg tgacgtagcc tttgcggacg tggcggccgt cgggggtgag
     7681 gaaggcggga tcgaaatagg cgccctggcc cggcagcacg atccagtggt cgaatcccgt
     7741 cggttcgctg cccaggtgcc atttgccgat catgcccgtg taataccccg ccttttgcag
     7801 gtacttcgcc acggtgggct gcgagccgtc gaagcggttg aaggttggca cgccgttcag
     7861 gtggctgtat ttgccggtca gaatcgttgc ccggctcggg ctgcagatcg agttgacggc
     7921 aaagcaattg gcaaaacgca ttccctcccg ggccagccga tccatgtgcg gcgtctggtt
     7981 gacgcggctg ccgtaggtgc tgatggagtg cgcggcgtgg tcatcggaca tgatgaacag
     8041 gatgttgggg cgggcggggg aggattggcc gagcgccagc gttagcggca gcagccaggc
     8101 tgacagggcc gcgggcagga gaggtttcat gctgccgatt ttacccgcct cccggcgggg
     8161 gacaaccctg aatttgcggc gtcagcaaat ccgcgtgttc tttgagccag cgttccgcct
     8221 cgcggtagtt cgggcagagg cgggccactt ccgcccaaaa ccgccgggaa tggttcattt
     8281 cccggaggtg cgccagttca tgcagcacga ggtaatcgcg gacccagggc ggtgtctgca
     8341 ccaggcgcca gttgagggag atggtgccgc ggggcgagca ggaaccccag cgcgagcgct
     8401 ggctgcggat ggtcacgcgc cgcacggaca aatgatgccg ggcggccagt tcgcgcacgc
     8461 gcgccggaag ttcgagcgcc gccagccgcc gcaggtgttg ctcgacggcc gggcgcaaat
     8521 cgccgtccat cccggctacg gggacggcct cggtcccgaa gcggatgacg tttcccgcgt
     8581 ccgtgcgcgc ggcctccagg cgcacgcgct cgccccggaa gaggaactcg gttcccgccg
     8641 gccagcgcgc cgggggctgc ggcgcgcggg cctgccgcaa aagctgccgc tcgatccagg
     8701 gcgcgctccg ggccgcaaaa cgctgggcct ccgccaccga gccgccccgg ggcacggtga
     8761 cgcgaaccgc gccgtccgcg cgcaggcgca ggacatagcg ccgcgcccgg cggtggcgga
     8821 caaaccagag ccgcacgctc cgggaaccga ccgccaccca cgccgacgcc ggtgcggcgg
     8881 gcggggggcg gcaccagggc cattccagtt gcactgttga ttttatacca tgcggccggg
     8941 cttggggcag caatcaatcg caacccggag ggcgggctgt gccggtgggc ggagattcgg
     9001 cttgtcctgg cggggcggcc tggggcttta tcgttgcatg cactggattt atctggccgt
     9061 cgcaatcgtc tccgaagtca tcggcaccgc cgcccttaaa gccgcgcagg ggttcacccg
     9121 cggggttccc acgctgctcg tggtaacggg ctacggcatt tctttttact ttctttcgct
     9181 ggttctgcgc accattcccc tgggcgtttc ctatgccatt tggtcgggcg ttggcattgc
     9241 cctggtgact ctggccggct ggtttattta tgatcagcgc ctcgatctgc cgacgttcct
     9301 gggcatcggg cttatcattg ccggcgtgat cgtaatcaat gtcttcggcc agtccgcggc
     9361 ggcctgaact ggcgcccggt ccccgccgcc gtcggccgtt acttcaacgg caggtaaatc
     9421 tctgttttca ggttctccgg ccgcgtgtgt cccggcgagt tcaaataccg ttcaaagcac
     9481 ggcacgtccc gcaattcttc gccgctgccg ggaagccact cccccaaaat atgctggtag
     9541 gcccccagca gattctcata cggcccccgg tgtagaaaca ccgcgtagcg cccgccagcg
     9601 atggtctgca cgcccacctc cccttccggt gtcaccggcc ggcccccgtg cggcacccag
     9661 gttagcccgt tcgggcgtcc tttccgggaa ggggtattgg gtcacggttt gcggggagtt
     9721 gttttcggcg cgcggccggg cggtctttca tccccggcgg gcgcttaaat actttgaccc
     9781 ctttcgcaac tttccctact ctggtgtgta tcaaaacagt aaagacggca tagatggcgt
     9841 ggtgatagat ggctttttta tcgctcaagc agtttttagc ccaggtgggc ttggccactc
     9901 ccgagcagtt cgatgaatgg aacaaggcct ggaaggtggc ggcggccagc ggttcgcagg
     9961 agccgctgtt ggcgtttatt tgccgggagc gggggctggc ggaggatgtt tttctccagc
    10021 aattggccca caaactccag tggccgtatt tggacctgcc ccgggtaacc atccccgccg
    10081 aggtgcggaa ccggatttcg accaaggtcg ccttccagca cacggtgttg ccgacgcagg
    10141 ccggcgaggg cgggctgcag gtggtggtga gcaatccgtt tgacgcggcg atgctgaacg
    10201 cggtgcgctt cgacgcgcgc ggcccggtgc aatttgcgct ggcgcccaag gcggaaattg
    10261 aaaaggcgct caagaaatac tacggcgtcg gggccgagac tctggatgaa atggcggaag
    10321 acgagccgct ggaactgctg gtgggcgagc agaaggaaat caccgagggg gaccaggagg
    10381 ccagcgtcat caagttcgtc aaccaggtga tctgggaggc gttcaaggac cgctcgacgg
    10441 atatccactt cgagccggcg gaggatgagc tgcgcatccg ctatcgcatt gacggaatcc
    10501 tgcaccagac gcccatgccg ccgcagctca agcgctacca ggcggcgctg atctcgcgca
    10561 tcaaggtaat gtcgggcatg aacatcgccg agaagcgcct gccccaggac ggccgcatca
    10621 atgtgcgcat caagggggag gagattgaca tccgcgtttc gacggtgccg acggtttacg
    10681 gcgagagcgt gtcgctgcgg ttgctgacgc ggggcaagat tttcctgagc ctggacaagc
    10741 tggggttttc gccgctggag gagaaggcca tccgcgagat catcgtcaag ccgcacggca
    10801 tcttcctggt caccgggccg accggttcgg gcaagtccac ctcgctctac gcgtttttga
    10861 gcacgatcaa ctcggtgcat aaacgcatca tcaccatcga ggagccggtg gaatacgagt
    10921 tgaagggcat caaccagatt gcggtgcggc cggagatcgg gctgacgttt gccatggggc
    10981 tgcggcatat cctgcggcag gacccgaatg tgatcatggt gggcgagatt cgcgacctgg
    11041 agacggcgga gattgcgatt cgcgcggcgc tgacggggca cctggtgttc agtacgctgc
    11101 ataccaatga cgcgccgagc gcgttcacgc ggttgattga tatgggcatc gagccgttcc
    11161 tggtcgcctc ttcggtggaa tcggtcatgg cccagcgcct ggtgcggacc atttgccccc
    11221 actgcaagca ggagcagaag gtcgagaagg attatttgcg ccggattgga tttccggagc
    11281 acgaaattga gacggcgaag ttctggcacg gggtggggtg cgaggattgc cgacagcttg
    11341 gctaccaggg gcgcatgggc atttatgaac tgctaatcct caacgagtcg ctgcgcccgc
    11401 tgattctgaa ccgggcgccg gcgtccacga ttgcccagca ggccatcgag tttggcatgc
    11461 gcacgctccg cactgacggc tggaacaagg tccgggccgg gcagacgacg atcgaggagg
    11521 tgctgcgcgt gacccagatc gaggagcatc ttgacgcgct ggccgaggat cgcaaggtgg
    11581 cggcggggat taagcggtga agacctggcg aaccatcacg ggcggcggcg ggtgcaggtt
    11641 ttctttctgc gcgcttttcg gccgggccgg agggcgattg atcgcggccg cgcttctggg
    11701 ttttgggctg gctggttgcg acccggcgcc acattcggcc gccccgcccg cgggcaccaa
    11761 cgcggcggcg ccgcgtcccg ccgccgggtc tccggcaacg gcgttacgga cgccgggcgc
    11821 gcgaaccaat gcgcctgccg tcgccgcgcc ggcaactgcc cgggttcaga cgcccggcgc
    11881 accgcttccg cccgctccca aaggcccggt gccgccggct cccgcgcggc ccgggaccgg
    11941 caccaacgcc ctggcgtccg ccgttccgcc cggggtcggc accaatgccg cttcggcgac
    12001 gggggcgggg tctggaatcg ccgatcaatt gcgcgccctg acggccagcc gcgtgttttt
    12061 cccggtcctg gggattattg tcctgagcct ttgcctggtc gcgatgtggg tctggaaacg
    12121 agtgaaggcc aaagagccaa agcccgcacg ggccgagcca gcggcggcgg cgaagacggc
    12181 gagcccggcg gcccgaaaga agccggggaa gagttcggtt cattcctgca acgtgcttca
    12241 ggtggacgcg caggcgcggc agctctggca atttgacgcc aaggggcgga atttcgtgct
    12301 gaaccgcgag catacggtgc cggccggcaa ggcgttgccg gcgtccctgg tggccaagga
    12361 ttggcggtcg ctgtttcaac cccggctcaa cgtggtctgg ctgccgccgg agcgggtttt
    12421 tctgcgggtg gtgcagttgc cggtcagcga tctggacgag accctcgcga tggtggaact
    12481 gcaattggag cggctgtctc cgttcccggt cgcgcaaatc gtctggagca tccagatcct
    12541 gccgccggcc gagggcaatc tgcagacggt gatcgtgctg atcgcgtcgc gcaacgaggt
    12601 ggaggagttt ctcggggcat tggaggggca ggggtacctg gccgaccggc tggagctgcc
    12661 gttgctggac caattgcagg cgaccgccat taccgccgac ggcgcctggg tttacccgga
    12721 accctccggg agccggaata cggcgctggt tgcctggtgg tatggcgggg tgctgcagaa
    12781 tctcgggctg ctcacgctgc cgccgtccgg gcgcccggag agcctgcggg aacaattgct
    12841 gcagatggcc tgggcgggcg aattggaagg ctggctgact acgccgccca cctggcattt
    12901 ggtggccgat ggcccggccg ccgccgactg ggaaccgccg ctgcgcacgg ggctggcgca
    12961 gccggtcgaa ctggtggagc ccgtcacggt ggcggggctg gccgctttga cggccacgcg
    13021 agcgacgcgg gccggggcgc aggccaatct gctgcccgaa gagtatgcaa cccgctaccg
    13081 ccagcagttt gtggatcgcc tgtggatgcg cgggctggga gcaatcgtcg cgctttacct
    13141 ggcgggcgtg ctgatttatt tcgcccggct ccaggtggaa ctttatcgca cgcacgccgt
    13201 cgagcagcag gcggccgaac gcgggccgat cttcaccaac gccctgcaac tcaaagcgaa
    13261 actccaggtc ttgaaagacc ggcaggactt gaagttcgcc gcgctggatt gctggaaagc
    13321 ggttgccgaa ctgctgcccg acggggtcac cttggaaagc tacaacttca gcgaagggaa
    13381 aaagctgaca ttgagcggca ccgcgccggc ggaccagacg aaattgctca cggcgttcca
    13441 tgacgacatc cgcaaggtgg cgcccaacgg ccagctttta ttcaacccga acaccccgcc
    13501 gctgacctgg gagcggcggg gcgagacggt gagctggcat tgtgtgctcg aattgcgacg
    13561 gacggaggcg ctatgacgag ctatctggat cgccttaact tgcgcccgtt tgagaagcgc
    13621 ctggtggtcg tggtgggcat tgtcctgttt gtagtgctca acatgtggtt tgtggtgccg
    13681 catttctccg atttgagcga cgcccagcgg cggcgggttg aggcgcagaa gaaactcgag
    13741 cgctggcagg cggaaattga ccaggcgcag aaataccagg cgggcatcaa caccttcgcc
    13801 cggcagggcc tggatgtgcc ggtcgaggac cagcaaaacc agtttgtgcg ggtcattcaa
    13861 gatcagcagt cgcagagcgg cgtaggcatc cagagcctgg gccggacctc cacccagacc
    13921 aaccaattct ttctggagct gacccagttg attaccgtgc aatcccagga agcgcagttg
    13981 gtggacttct tgtatagcct gggcgccggc gattcgctga ttcgcgtccg ggacctgtcg
    14041 ctgaaaccgg atccgccgcg gcagcaactc agcggcatgg ttaaactggt ggccagttat
    14101 cagaagaacc caccccggaa aacggcgccc gacgccaaaa cgccgccggc gtcggccaaa
    14161 atggcaactc cttctcctac tcccaatccg tgaacgcggt cttttttgct ttgctgttat
    14221 tcggtctgcc tttgtgggcg caatctcctc cggttgccac gcctccgccg gcggttgcgc
    14281 ccggagatcc cgccgccggg gtcgctccgg ccgccgcccg gtcacccctc gtcgtccccg
    14341 aacccccgct tccgcccgat attggcagcc gcagccggcg ggacacgaat acgacccggc
    14401 ggctctcgac gtcccgtcgt gtaggggttg ccgccgtcac caatgcgccg cccactggaa
    14461 cgggggtccg gccgactgag cccactcccg gttcggagac gcgagttcgt ccgctggcgc
    14521 cgccgagcac tcccgccccg caagccgtga gccgggcgga taatgtggcc ggaccaaccg
    14581 caaccccaac gggagttatc acgcccgcag tgggaccggc tggggcagcc gttcccccgg
    14641 gaccgggatc ggggttgcca tcgaggggtt cggctgcgtc attggaggcg ccgttgccgg
    14701 aggggatgat agactttcgg gaagctgatt tgaagcaggt gctggatatc tacagtgata
    14761 tggttcaacg caccgtgctg cgtccggcga cgctgccggc gccgacgatt acgctgagca
    14821 cgcgggggca tttgacgttg ggggagggga ttcaggcgtt ggaggcggtg ttggccctta
    14881 acggcatcgt catggtcaac gtgggggaga agtttgtcaa agcgctgccg gaggcgcagg
    14941 ggatcagttc gggggcgcgc tttgacacca acagcgcggc gcagatgccg gagttggggc
    15001 agtatgtgac gcatatcgtg cagttgaagt atgtgcggcc gagcgagatg gtggcggtgt
    15061 tgacgccgtt tgggaagatt cccaacgcga ttttgccgat cgacagcagc cagattttgg
    15121 tgttgcggga ttacacggag aacatcaagc ggatgctgga gttgatcgag aaggtggatg
    15181 tggtgatacc gtcggagtat gtatcggaag tgattccgat caagtatgcg caggcggtgg
    15241 agattgccaa tgcgctgagc agtttgagtt ccgggggcgg gggagcgacg gtggggagca
    15301 cggggacggg gaccagccgg agcagccgga gcacggggat caaccggccg ggcagcatga
    15361 cggggatgag cgggtatccg gggcagcagc cgatgacggg gatggcgacg ccgccggggc
    15421 gggcaggcac gccggcggcg ggggcgggag ggagcacgtt tacgcagcgg ctgcagaaca
    15481 tcatcaaccg ggcgtcgcag gagggggaga ttcaggtttt ggggcagacc aagatcatct
    15541 cggacgagcg gaccaactcg ctgctggttt atgcgacgcg ggaggacatg aaagtcatca
    15601 aggagattgt gggcaagctg gacgtggtcc tggcgcaagt gttgatcgag gcggcgatca
    15661 tcgaggtgac gctgagcgac agccgggatt tgggatttag ctatttgcag cggccgcagc
    15721 atacgggcaa gtggacgggg gtgggggcga tcaactataa taacatgctg cagccggggg
    15781 gatttgcggg cgggggcggg accaacaacc cggtggggca gggatttaat tatttgatgt
    15841 cctggaacca ggatttggat gcgacggtga cggcgctggc gtcggacagc cgggcgcgga
    15901 tcttgcagcg gccgcggatt cagacctcgc acaacgagcc ggcgagcctg tttgtggggg
    15961 aatcgcggcc gtatccgacg tccagttatt acgggggcgg ggcgtatggg ggatattcct
    16021 cgatcaacca gttgcagatc ggggtgacgc tggaagtgac gcccttgatc aacccggacg
    16081 ggctggtggt gatggagata cagcagcaga ttgacagttt tcagggcaac gtgacgattc
    16141 agaacgtggg ggatgtgccg attaccagtt ccaagatcgc ctcgacgaag gtctcggttc
    16201 gggatcatga cacgattatc gtgggggggt tggtggagac gcaaaagaac aagaatgcgt
    16261 ctggggttcc gtatttgaag gacgtgccgc tgctgggtta tttgtttcgg agcacgcatg
    16321 cggcggagac gcgcaaggag ttgctggtgt tgattcggcc gacggtgttg ccgacgccgg
    16381 aggtggcggc gctggcggcg acggcggaga agaacaagct gccgggggtt cgggagacgg
    16441 agcacgagtt gcggacggag gaaatccgcc gcctcaaagc cgccgataaa gccgaaaagg
    16501 gagggagacc gtaatcggca gcatgccttt cggagcggtc gcgcggaaag agtgagaata
    16561 tgtgcaacag gattcagata acgccgaaga gcaggcgaag ccacccctcg gcggggcggg
    16621 ccgccgtcgc catgcttgtt ctgctgggag cgttgctggc cggggggctt ccggccggag
    16681 gagcagtccc tcctgaaata gacccgggcg ccctcgaaga agacgacggg acactcgatt
    16741 tcggagaccc cacgaacaac ggcaacctgg tttccgattg ttcccagtcg cagattcagg
    16801 aattccttgc ggctgtgcgg gagggcggca cggtggagct tacggattgt ccccggctgg
    16861 tcttgacgaa cgccgtcgcc atcaccaccg acgttaccct tcggggggac gaagacggca
    16921 agacaaccat tacgggcaac aacctgagcc ggatgtttac tgtttatccg cgcggttgcc
    16981 tgacgctgga aaacctgacg ctgcagggcg ggctgagtcg ctatggcgga gccatttata
    17041 atatggcggg cgggcgggtc gtattgaata attgccgcat cgttggcaac caggccgtcg
    17101 gccgcaacgg agtgaatggc cggaacggcg ataatgaccc gaattacgga aagaaaggca
    17161 aaagcgcatc ttccgggcag tcgggggcgg ggggcggaat ctacaatctc ggcaacctgg
    17221 aggcgaccga ttgccaattt acagacaatc gcgccaaggg tggacatggc ggcgacggtg
    17281 gtaatggcgg cgaggggcgc taccaggggg gcaacggtgg cgacggcgga gatggcgcct
    17341 tggcttttgg cggcgccatc tacagcgagg gcaggctttg gctgactaat tgccagttcc
    17401 ataccaacgc cgccacggga ggcaacggcg gccgcggcgg gagccccggg acgagtgttt
    17461 ttgccggcta cgacggcacg ggcggcgccg gagccgaggg gtcgggcgcg gccatttaca
    17521 gcgccggtgg catggtgctt tccaattgcg ccttctttgg caataccgcc cgggggggaa
    17581 atagcgccgc ggcgggcgag gcgggcggcg gttacggcgt gaatggcgcc gacggcggct
    17641 acagttatgg cggggcggtt tgcctgctgg gcgacggccg gctgatccac tgcgattttg
    17701 ccaacaatac cgccgctggc ggcatgggcg gcgatggcgg cccggggcgc tacgtcgccg
    17761 gcaatggcgg gaatgggggc aaagctgtcg gcggctgcct ttacaactac ggcggcgccc
    17821 ggctcgtgga ttgttttttg acgaactgtg cggcgattgg cggcagtaac ggggtcggcg
    17881 gaagcgcccc atttgccggc aaagacggtc gcccgcgagg cgctgatgat aagtctatgc
    17941 gggtcagaga atcggagacg atccgtgcgg tcagtttgcc ggaaggcgcg gttccggcag
    18001 ctacgggggt tgccaccgtt cttctcgccg aacatcccgt ggctgccgag agcgcccgaa
    18061 atacgccgcc gcaagccggt ctgtcccccg ctgacagcaa ggccgcaaac ttgccgcccg
    18121 ggctgcccgc ccatctggtc ggaactaatc ttctcgtaaa accccgtgct tccacggcgg
    18181 aaacgaatgg ctctcctccg gcacttccgg cggcggggaa tcgttcgcca tcctctccag
    18241 gaggcccgtt tacacccgtg gcggggccgg ccgttccccc gggaccggga tcggggttgc
    18301 catcgagggg ttcggctgcg tcattggagg cgccgttgcc ggaggggatg atagactttc
    18361 gggaagctga tttgaagcag gtgctggata tctacagtga tatggttcaa cgcaccgtgc
    18421 tgcgtccggc gacgctgccg gcgccgacga ttacgctgag cacgcggggg catttgacgt
    18481 tgggggaggg gattcaggcg ttggaggcgg tgttggccct taacggcatc gtcatggtca
    18541 acgtggggga gaagtttgtc aaagcgctgc cggaggcgca ggggatcagt tcgggggcgc
    18601 gctttgacac caacagcgcg gcgcagatgc cggagttggg gcagtatgtg acgcatatcg
    18661 tgcagttgaa gtatgtgcgg ccgagcgaga tggtggcggt gttgacgccg tttgggaaga
    18721 ttcccaacgc gattttgccg atcgacagca gccagatttt ggtgttgcgg gattacacgg
    18781 agaacatcaa gcggatgctg gagttgatcg agaaggtgga tgtggtgata ccgtcggagt
    18841 atgtatcgga agtgattccg atcaagtatg cgcaggcggt ggagattgcc aatgcgctga
    18901 gcagtttgag ttccgggggc gggggagcga cggtggggag cacggggacg gggaccagcc
    18961 ggagcagccg gagcacgggg atcaaccggc cgggcagcat gacggggatg agcgggtatc
    19021 cggggcagca gccgatgacg gggatggcga cgccgccggg gcgggcaggc acgccggcgg
    19081 cgggggcggg agggagcacg tttacgcagc ggctgcagaa catcatcaac cgggcgtcgc
    19141 aggaggggga gattcaggtt ttggggcaga ccaagatcat ctcggacgag cggaccaact
    19201 cgctgctggt ttatgcgacg cgggaggaca tgaaagtcat caaggagatt gtgggcaagc
    19261 tggacgtggt cctggcgcaa gtgttgatcg aggcggcgat catcgaggtg acgctgagcg
    19321 acagccggga tttgggattt agctatttgc agcggccgca gcatacgggc aagtggacgg
    19381 gggtgggggc gatcaactat aataacatgc tgcagccggg gggatttgcg ggcgggggcg
    19441 ggaccaacaa cccggtgggg cagggattta attatttgat gtcctggaac caggatttgg
    19501 atgcgacggt gacggcgctg gcgtcggaca gccgggcgcg gatcttgcag cggccgcgga
    19561 ttcagacctc gcacaacgag ccggcgagcc tgtttgtggg ggaatcgcgg ccgtatccga
    19621 cgtccagtta ttacgggggc ggggcgtatg ggggatattc ctcgatcaac cagttgcaga
    19681 tcggggtgac gctggaagtg acgcccttga tcaacccgga cgggctggtg gtgatggaga
    19741 tacagcagca gattgacagt tttcagggca acgtgacgat tcagaacgtg ggggatgtgc
    19801 cgattaccag ttccaagatc gcctcgacga aggtctcggt tcgggatcat gacacgatta
    19861 tcgtgggggg gttggtggag acgcaaaaga acaagaatgc gtctggggtt ccgtatttga
    19921 aggacgtgcc gctgctgggt tatttgtttc ggagcacgca tgcggcggag acgcgcaagg
    19981 agttgctggt gttgattcgg ccgacggtgt tgccgacgcc ggaggtggcg gcgctggcgg
    20041 cgacggcgga gaagaacaag ctgccggggg ttcgggagac ggagcacgag ttgcggacgg
    20101 aggaaatccg ccgcctcaaa gccgccgata aagccgaaaa gaacaagccg gcttcctccc
    20161 ggctggcgcc ctgattcagg ccgggcggcc cggcggcccg gggctttgcc ggaagcgaaa
    20221 aagaacctgg ggccgggcgg ctcgccaggt tttctggctt tgccgcgaat taagtgtata
    20281 ttgcgcgtca gacagagaac ttgaatccgc gatttctcca accagattgg gcgcgcatga
    20341 agaacttctt ttcgggccgg tttaggccgg gtgccatgcg aggtggcggc ctcggcgcgg
    20401 ccctgctatt gctgctggcc gtttctgcct ggagcgccga caccttcaac tgggatacca
    20461 accgcaatcg agtcagcgcc gacattaaag ccacccaact ccggccgctg ctggagcaga
    20521 tcgccgccgt caccggctgg cacatcctgc tggaacccga caccacctgc gatgtctccg
    20581 ccaagttcga gaacctgccc ccgggagaag cgctgcgcct gctctttcgc gacctgaact
    20641 ttgccctggt ccccgacacc aatgccagcc cccgcctgtt cgtcttccgc accgccctgc
    20701 ggaacgcaac ccaatccatt ccacccgccc gcgccgccga atcagccagc cagggcaagc
    20761 tcatccccaa cgaactgatc ctccggctca agcccggcgc gaaaattgac gaactggccc
    20821 ggttgctggg cgccaaagtc accggccgca ttgacagcct caatgcctac cgcctccagt
    20881 tcccggacgc cgccgccgcc gaggccgcgc gccagcagtt ggccgcccat ccggaggtcc
    20941 tctccgtgga cagcaactat tcgattgacc ggccgcccgg cccggcggga acgtcggctg
    21001 caaacctcct gccgccgccg cggttgcagc tcaaaccgcc ccccaacgac gggcgcatcg
    21061 tcgtgggcct ggtggatacc gccgtccagc cgctggggaa tgacctggac gcctttctgc
    21121 tcaagcctct ctcggtggcg ggcgaggcgc aactggatcc ccatcttccc tcccacggca
    21181 cttcgatggc ggagaccatt ctgcacagtc tcgccgccat gaccaaagac ggctccaccg
    21241 tccggatttt gccggtggat gtctatggcc ccaaccccac caccagcacc ttcgatctcg
    21301 cccggggcat cgctgaagcc gtcaatggcg gggccaatct catcagcctc agcctcggca
    21361 gcgagggcga aagcccgttc ctgcacaacc ttatccagga cgcccacgcc cggggcatcc
    21421 tcttcattgg cgcggcgggc aactctcccg tcaccacgcc gttctatccc gccgcctacc
    21481 ccgaggttat ggccgtgacc gccattgacc agggccagat tgccagctac gccaaccgag
    21541 gcagctttgt ttccttcggc gccccgggca ccagcattat ttatttcaac aaccagccct
    21601 actacgtcac cggcacttcc gccgccgccg ccttcctcag cggcatcgcc gccggctata
    21661 tggattccac ccgcagtgat gcccgccaga tgcagacgtt cctccaaagc aactttggcg
    21721 tgaagatcac ccccagccgc tgagcggcat ccccggccgc tgccccgcag gcccggaggc
    21781 aactcgtggc ttgaaacggg cgggcggagg gttgaaactc cgggggtgcg ttggaagaaa
    21841 gtcacattga tcggcgtcgg cttgctgggc gggtcgctgg ggctggcgct gcgggaacgc
    21901 gggcgggcga agtcagtggt gggctttgtc cggcgcgccg ccagcgtggc cgagtgtgag
    21961 cggctcggcg cggtggacct ggccacccgg gacctggcgc gcgcggtggc gaaggcggag
    22021 ctggtggtcc tgtgcactcc cctggcccgg atgcgcccgc tggtggagac gatgctgccc
    22081 ttgctctcgc cgggggcggt cgtaacggat gtggggagcg tcaaaggcag cgtggtgcgc
    22141 gacctggagg cgctggtggc gcgggcgggc gcgcacttcg tgggcagcca tcccatggcc
    22201 gggagcgagc ggatgggagt ggcggcggcg cgcgcggatt tgtttgccgg cgcggtatgc
    22261 gtcgtgacgc ccacgcgcca atcaaacccg gcggccgtgc gccaggtgga gcagctatgg
    22321 caggcggtgg gcgcccggcg gctgcggctt tcgccggcgg cgcacgatca aatggtaagc
    22381 cgatccagcc acctgcccca cgtcgtggcg gcccagttga ccaacctggt tctcggcccg
    22441 aaacgcccgc gcgcgcaggg actgctctgc gccaacggat tccgggactc gacgcgcatt
    22501 gcggccagtt cgccggagat gtggcgggat attgcgctgg ccaaccggga gaacctgctg
    22561 cgcgcgctgg cagggttcgc gtcgggcctg cgggattttc aacgcgcgct aaaagccggg
    22621 gatgcccggg ccgtatcaaa gttcttcgcg caggcccgga agcggcgcct ggcctgggcg
    22681 cggggagccg gagcccggcc ggcggaatga ggcgggaaag ttagctctcc ggcggcatgg
    22741 ctctgcctga actcattgaa atcgttccgc tcgaccggcc ggtgcgggcg gaaatcaccg
    22801 tgccggggtc caagagcatc accaaccgcg ccttgatcct ggccgcgctg ggcccgggcg
    22861 aaaccacctt gccgggcgcg ctctggagcg aggacacgca ggtgatggcc gcggcgctgc
    22921 gggaactggg gttcgcagtg aacgtggccc ccgacccgcg ggagccgggc aaccgcgtga
    22981 tcaccgtcgg cggccagggc ggcagggtgc cgcgcgccgg caccgtggag cacccccggg
    23041 agcttttcgt gggcaatgcg ggcacggcgg cccggttcct ggcggcgctg gtgtgtttgg
    23101 gccggggcgt ttaccggctg cggggcgtac cgcggatgca ggagcggccc caggccgcgc
    23161 tcttccgcgc gttgcgcgag ctgggctacc gggtcgagtc gcccaacgac cggctgccgg
    23221 cagtgattca cggcgccggc gcccgcgcgg cgcattgcga ggtgagcatt ctcgaaagct
    23281 cgcagttcgc ctcggcgttg ctgctgtgcg ccgcggccgg gaagtggcgg gtgacggtgt
    23341 cgggcgagaa tgcggaggaa tcatcctatg tggcgctgac gcgcaagctg gccgcggcgt
    23401 ttccccgttc cggcgggcag tttcccattg agcccgacgc ttccagcgcc agctactttc
    23461 tgggagcggg ccacttgatt cccgggtcgg cggtgactct tcgcgcgcct ccggccacgg
    23521 actggcaggt ggatgcggct tttccgcggt tcctgccgtt gccggcgagc ctctcccgcc
    23581 gggatgagct gggcgacagc atcatgacgg cgatcgtgct ggcgccgctg gcccggcagg
    23641 cggtgcggtt caccgacctg ggccggctgc gcgtgcagga atgcgagcgc gtgcaggccc
    23701 tgcgcacgga actgacccgg tgcggcgccg ccgtggcgga ggccggcgat acgctgacgg
    23761 tctttccctc cccgctccac ggcgcggaga ttgaaaccta ccaggaccat cgcatggcga
    23821 tgtgctttgc gattctggga ttgaaaatcc ccgggataag actcaagaat cccgcgtgcg
    23881 tgaagaagac tttcccgaac ttctttcaaa agctggcggc gccgccgccc cacggcctgg
    23941 gggcggaact gcgcgaggcg accagcggac gggtgctgcg cccggacgag ctgctggccg
    24001 aatgaaccgg ccccacccga ggtgaagccc atgccgcagc cccgcaagca ggcgccggtg
    24061 gtcatcgcca ttgacggcac gagcgcgagc ggcaagagca ccaactcccg gttggtggcc
    24121 caggcgctgg ggttcatcca tgtggatacg ggcgccatgt accgcacgct cgcctggtat
    24181 tgcctccgcc accagattga cgtgcggaat gcgcgggccg tcgccgccgc ctgccgccgc
    24241 tggaaggcaa ccctccagtg cgccgacggc caggtttacc tggcggtgga cggctattat
    24301 cccgccacgg aaattcgctc cctcgaggtc agcgcggtgg tctcgcacgt ggcggccgtc
    24361 cccaaagtgc gggaatggat gaagaagaag cagcgcgaat gcctccagtt cggcaacctg
    24421 gtcatggaag ggcgcgatat cggcacgaac gtctttcccg aaaccgactt caagtattac
    24481 ctcgatgcga aactggagga gcgggcgcgg cggcgggccg ccgacggcct ccaggaaaac
    24541 ctggcgcagc gcgaccagcg cgacagccag cgcgcggtcg cgccgctcat gatcgcgctg
    24601 ggagccaagg tcatcaacaa ctcgcaaatg accccggagc agaccagcgg aaccatcctg
    24661 gccgacatcc gccagcggct ggccgccgcc gcccgggccc cggaccgatg agcgcccatg
    24721 aacttctggt atttcctcgg ctggtgcgcc taccgaacgc tcttccggtt ttacttccgc
    24781 tggcgggtgt ataacgccgg gcgggtgccg cgccagggcg gggtcatcct ggcgtcaaac
    24841 cacgccagtt acctggaccc gccgctggtg ggggccggcc tgcggcgcgg catccattac
    24901 ctggcgcggg aaaacctgtt ccggttcccg gtcatgggct gggttttgcg ccagtggcag
    24961 gtcgtgcccg tggaccgcga cggcggcggg gccaaagggc tcaaagccat cctggaccgg
    25021 ctgctggccg gcggcgccat catccttttc ccggaaggca cccgcacgcg cgacggccgg
    25081 ttgcagccgg cgcgctccgg catagggctg acggtgatca aatccaccgc gccggtcgtg
    25141 cccgtgcggg tctttggcac ctttgccgcg tatggccggc atctccggct cccgcgcccg
    25201 ttccgggtgg cggtcaaata cgggccgccg atggactttg ccgcgctccg ggcggaagcc
    25261 cagttatgct ccaaagcgcg gttgaaggaa atctaccagc aggtcgccga tgacatcatg
    25321 gcggccatcg cccaactcgg gccgtatgaa gacctgaccc ggtttccggc taaaacaaac
    25381 tcgcctgctt cccgcccgcg ggcttgaccc ccagcttctg gtagctgagc tcggtggcca
    25441 cccgcccctg cggggtgcgc ttgaggtagc cttccatgat caggtaaggc tcatagacct
    25501 cctccagggt gtccggctcc tcgcccaccg ccaccgccag cgagctcagc ccgaccgggc
    25561 cgccgccaaa cttcacgatg accgtttcca ggatccgctt gtccatctcg tcgaggccgt
    25621 tctcgtcaat ctccagcatg gccagggcct tgtcggcaac ctcgagggtt atgtggccgg
    25681 tgtgccggac ctgcgcgtag tcccgcacgc gccgcaggag gttgttggcg atgcgcgggg
    25741 tgccgcggct gcggcgggca atctcccggg cgccctcctt ctcgatctcg acgttgagca
    25801 gccgggcgga gcgcacaaca atctgctgga ggtcctcggc atggtagtaa tccagccgct
    25861 cgcgaatcgg gaagcgggtc agcagcggcg cggtcagcag cccgctgcgc gtggtcgcgc
    25921 cgatgagggt gaagcggggc aaattgagcc gcacgctgcg cgcgttgggc ccctggtcaa
    25981 tgatgatgtc cagcttgaaa tcctccatcg ccgggtaaag atattcctcg atggtcttct
    26041 ggaggcggtg aatctcgtca atgaacaaca cgtcgccctc ttccaggttg gttaacaggc
    26101 cggccaggtc ccccgccttc tcgatggtcg gcccgctggt gcttttgagg ttggcgccca
    26161 tcgccttggc caggatatgc gccagggtgg tcttgcccag cccgggcggc ccgctgaaaa
    26221 gaatgtggtc aatggcttcc cgccgttgct gggcggcggc gacggtgatt tccaggcgct
    26281 ccttcacccg gagctggccg gtgaactccg caaagagcga ggggcgcagc gtcatttcca
    26341 gcgcccggtc cggctgggcg agcgcctcag agattctgtg ttcggccatc gccccaagga
    26401 tggccaaagc cccggggcgc gcaagacgga aagtcgccgc gccgccccgg ccttatttca
    26461 ccgccagcaa tcccagcccg gtgcaggcga tggcgatgag cgcggcgacc agcgactcgc
    26521 ccgcgatcag ccccgaggcg accggcacgt agtaaaggtc cgcggcccgc cggcgcgcgc
    26581 ggctccacag cagcaccagc accgcgccga gggcaaagga aaggctgttg gcaaacggca
    26641 tgacccacgc cagccccagg cccatcgccg agggcagata cggccgggcg cgcggggcca
    26701 gcttcgccgc caggggcagc agcaccccga tcagggcgcc gccgacaatg gcccacagcg
    26761 ccgtcaccgg cagcatgtgg acgccctgcg tgagcaggtc cgccaccgcc ttccacatga
    26821 aggtcgcggg cgggttgaag gcctccaggg ccttcggatt cggcaccagg gcataccacg
    26881 ccggcacgat ggccagggtg ccgaaaaaga tgcccacaaa ttgcgcgagg aactgcttgc
    26941 gcgggttggc ccccaggatg tagccgctct tgaggtccgt gagcaaatcc gccgccgccc
    27001 cgcccgccgc cgccgtggcc cccgccgcca tgaggttgat ggaggccacc ccgcgcgcgc
    27061 ccggcagcac ggcatacaat agctgcgtca ccttgcccat cgcgcccacc ggcgtcgtgt
    27121 cggtctcgcc ggtcgcccgg cagcagacca gcgacaccac aaacgccagc gcgaccgcaa
    27181 tcaggcccag gggaaccgag atgtggaaag ccagatactg gatgatcacc atgcccaggg
    27241 tgatgggaac cagcccggcc accagccagg aaaccggcac ttcaatcgcg tccatggccg
    27301 cgctgtgcgc cgtgcgctcc ttcttccgaa acaggctgaa cgcgcgcgcc agggtgcgcc
    27361 actccaatgc cacggtggcc aggctcgaaa agaccatgat agccgtcccc ccccacagcg
    27421 cccagcggat gggattgaag ctgccgtcgg gcaggagggt gaacgacggc acatatcccg
    27481 ccaccccggc gtgggtcaga tcgcggagca ggacgcgcgg ggcgaccacg aaatacagca
    27541 gcgtggcgcc cagcagcatc gagagcgaaa cccgcaggcc ggtaatcatc ccggcgccga
    27601 tgagcagcac gctgggctcg aagaccagcc ccgccatctg gccgtgcggg atcgggctca
    27661 gccagcgcgg cgccgccagc gcctcgggaa gggacagcac ctggtgcagc cgctccagcc
    27721 aggcctgcgg ccggcccgtg ccgcggaatt gctcgaccag cgtgccgtag ctgcgcagcc
    27781 cgcccaccag gccgccggcg gccagcgcgc cgatcagcgc gtaagccttc tgcagcgcct
    27841 cccggccccg ggaatagagg ctgcggagcg tctcggcggc ggcgatgccg ctgggaaact
    27901 tcagttgttc ctggttgatc atctggcgct tcatcgggat ggccagaaac acccccaggg
    27961 ccgcggcgaa caacgcaaac gcgcccacca cataccacgg ccgatgcatc ccctcgagca
    28021 gcagcaacgc cccaaacgcc gtgccaatgg tgccgccggt ggaataaccc gccgccgagg
    28081 ccgtggattg catgcagtta ttctccagga tggacatcgg gctgagccgc cccccggaca
    28141 gggcgcgcag gccgttccaa atcacaaacg acagcacgca cgccgtgatg gccacgccaa
    28201 aggcccagcc cagcttgagc gtcgtgtaaa gattggaaac ggacataaac atccccagcc
    28261 cgccgcccat cagcaccgcg cgcagcgtca actgccgctg cacatcgccg cgccccgtat
    28321 aaatcttctc gaaccactgc cgctcgattt cgtccggcgt gcccgaaaag cccttgatgg
    28381 gcaaatccag gtcgcgatcc gccgcgcccg cccccggcgc atccgccgct gccgtcgtgt
    28441 cattacaaac caaactcatc gccgtaattg ttgggggtgt cgccctgttc cgcgaggctt
    28501 ttcttgcccc cgccccgccc cggggcaggg ccttttgttg ccgcgcccgg gtttctgcag
    28561 ttgcgggaga aacgaggttt ccagacccaa gccgcttttc gggttcagga ggcggggatg
    28621 aaaacgtcta ttgcttcaag acggccgccc gaccctgcgg acagcgcaag caatcacctt
    28681 tcggcaacct ccgcctggga ggggaaatgt cctcgccagc cgtgatccca tcccacccaa
    28741 aaaccagggc acgcgacaac acccggccgc ccgttccggc tttgccgaac gctctcgtcc
    28801 tctccctccc gccttgaaac gttcttttac atcattcgca ggtcgtccat tcctccattc
    28861 cggctcaggc ccgcgcccaa acccgtccca cacgtcccag ccaacctaac gctcccatta
    28921 ggctgtgtac aacgggtgta caaaggaggc tcaaagggga ctcatgctta ttttccatgt
    28981 gctcctgccg ggcttccgat gtactcccgt tgtacatggc ttggaaggac ggagaaggaa
    29041 cggtcttgtg ggcggcggtg gaggtcgggg cggcggcggg cttcgatttc ttaacccttg
    29101 ccgtttcagc cgcttgctga gatttcaaca gcggcggcgg cggaacgatg gcgttgtgaa
    29161 cccggctttg gcctttccag ccgttccggg tctgaaccac ggcgataaac accttctgcc
    29221 ccggagcggg ccgcccaaac cgggctgtat agaggtccgt gatgtcgctt tgcccgttct
//
LOCUS       DAOPQG010000039        28903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2098_length_28903_cov_15.921970, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000039 DAOPQG010000000
VERSION     DAOPQG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 28903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28903
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2098_length_28903_cov_15.921970"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKI07_06225"
     CDS             complement(<1..514)
                     /locus_tag="PKI07_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50686.1"
                     /translation="MKILTSPRTGSLGAETYYQSPFGVCARRRTVPRDRPSERKAAAR
                     SYFGISSREWGLKLSEAQRERWNAAALHVPSRPWMGQYSHLSGQQFCVQINSTLRGLG
                     LAPVEEPPAPVVFGPNPVTGLEIVNDAEDGVRLRLNVGEAHEDIMVFGQPPCSAGRMK
                     PRRAYYLGLLG"
     gene            902..1657
                     /locus_tag="PKI07_06230"
     CDS             902..1657
                     /locus_tag="PKI07_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50687.1"
                     /translation="MKRQLRKIRQQLGSAAGWGLCLLAFSAGAEINLTSTTLAISGDW
                     QETWYLEADHQEHHNDGTFGDSTQSEPSLSLSVSSSPYGWAGASGILSAFGLRLEADA
                     APRGLSYEWGIGSLEPTTINTYATVATRFQVEQPALEIQWSGWAAFNYAMNEQDIYLE
                     LRDATTATSLLQWQASADWDTWGSGGFATVYPLTVTPAHEYELILAGWITAWDGKYAV
                     LEADVMIREVPEPASGWLLLLGWGLALAGRRGR"
     gene            2640..4139
                     /locus_tag="PKI07_06235"
     CDS             2640..4139
                     /locus_tag="PKI07_06235"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOC50688.1"
                     /translation="MIEIWNSLVNQVTDYAPNLLAALAVLVGGWIVALLISALLRKLL
                     VRTAIVNKLTKWIAGAESKPVPIEKWIGTLVFWLIMLVVLVAFFQILKLTAVTEPLQR
                     LVDNFTQYLPHLAAGLALALLAWLVAVLVRRVLSDGLKATEFDQRLGRIAASDQRPDQ
                     SVSKPIADTVYWAVLVAFLPAILGALRMNALLEPVNAMLSKFFAFVPNLAGALVILLV
                     GWFVARLVQRLAASLLASAGADRLAEKWGFAKALGTRRFSEVIGLVLQIVILVPVLIA
                     ALDQLHMEAVTRPASDMLQAMLIKVPQIVAASLILLVAWFIGRVLAGLVENLLGGLGF
                     NQILVKLGLSKKAQDGRAAPAAIVGALLIIGIMLLAAIAAAETLGYTRIAGVVENFLE
                     FAGKVLLGVLLFGLGLLLANMVFNVINASDSPRARGLAWTARIAIIILVGAMSLRATG
                     VADSIVNLAFGALVCGAAAAGAIAFGLGGRDFASRQLARWQNHTNEKHT"
     gene            4506..5705
                     /locus_tag="PKI07_06240"
     CDS             4506..5705
                     /locus_tag="PKI07_06240"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC50689.1"
                     /translation="MTHNIRRVLFPIPYLSELKTKTMKIRSHYLGLVLVVCFTGRIAA
                     AQTIDEKLEAMMKEQPTVGLSVAAVKDGKLIYTHSFGYKNLETKTPLSDNDIFRIASI
                     SKSFTSVGLMQMIQKKKISLDDDVSALAGFRIRNPRFPDKVITLRMVLSHTSSINDRQ
                     GYFDLVAINPDKNPDWAKCYNDYEPGSAYEYCNYNYNIAGAILERVSGERFDHYIAGH
                     ILKPLGISGGFNVNDLDPSRFALIYDYNRETARFILSPAAYAPRKKEISEYEMGFTTP
                     VFSPAGGLKISAGDLAQYMMMHMKNGKQNGKRIIAKKNAKLIRTPLSAKEHYGLGLLL
                     NPDLIPGVNMYGHTGGAYGLTSLMFFEPEKKFGFVAISNGNAPSYSSGRKAIKIMYEE
                     FIRARAD"
     gene            complement(5759..6616)
                     /locus_tag="PKI07_06245"
     CDS             complement(5759..6616)
                     /locus_tag="PKI07_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50690.1"
                     /translation="MPTSTGKGDLLRGARVYLSGPMDFVASRAAEKQSGWRNRVSQFL
                     QGMGVTVFDPWFKPDVRGLHEYGREDLKSGERIRRRWTYAGGKRGAQARAWCARQFWE
                     TLHIDLRMVDTSDFSISYCPTNIYSVGTPHEIILATMQHKPVLFVSPPIQFPALHELR
                     AHLADDPAGLALLARLEQEIPIKENPRGIPSLWYLPLVGGENFFDGFGFAAYRKRFGW
                     NVDIPIDHHERRFPPQRPLLPFLERLNRRLPRKWDGKLDRFVPDDDWLLWDFRAQTVR
                     GKHIESVRK"
     gene            6903..7823
                     /gene="atpB"
                     /locus_tag="PKI07_06250"
     CDS             6903..7823
                     /gene="atpB"
                     /locus_tag="PKI07_06250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOC50691.1"
                     /translation="MIFISWWLAVLFGTGLAGAASSALAADAAAVSAAAAPALEEGLS
                     PDAPRLGPGGASFINSSMVATWGVALMLILLVRRATRRMQLAPEGAQNFWEWLVEMLH
                     DFLEGIIGGKLVKKTFWFFATLFIFILFTNWFGLLPGVGSIGWGTQTPEGFHVERPLL
                     RGGNADLNMTFAMAMIFFVCWIVWAVQAVGVKGVFLDLFGPKGDTAGALKYLMIVVFF
                     LVGFLEVLSILFRPISLSFRLYGNIFAGENMLEAMGKLVPGLGWLLPVPFYFMELLVG
                     IVQALVFMLLTAVFTMLICVHEESPGGTGH"
     gene            7894..8109
                     /locus_tag="PKI07_06255"
     CDS             7894..8109
                     /locus_tag="PKI07_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOC50692.1"
                     /translation="MLTPMLAEGLAGNLHVGLAALGAAIGVGFIGMKASEAVGRNPGA
                     ATKILVQAILAIAFAEAIVFYAIFLVK"
     gene            8164..8715
                     /gene="atpF"
                     /locus_tag="PKI07_06260"
     CDS             8164..8715
                     /gene="atpF"
                     /locus_tag="PKI07_06260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOC50693.1"
                     /translation="MQPSFLFASIGSDLGQMARDTATRFGVDLPHFLAQCLCFAIVAF
                     VLQRYAYKPILTVLEERKRRIKESLDQAEKIKQELAHTQTRTQEMLLQAGQQAAQLIE
                     EARAAAAKVQEQETQKAIAAAQAIVDKARQATQAERERMLLELRREVGRLVVATTARV
                     AGKVLTAEDQSRLVEETNRELAA"
     gene            8742..9134
                     /locus_tag="PKI07_06265"
     CDS             8742..9134
                     /locus_tag="PKI07_06265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOC50694.1"
                     /translation="MKISKQARRDARELFRACCPDGLLDEARVRQALRQVLATRPRGY
                     LAILSHFRRLVKLDLDRRTARIESATPLSAAQQAALQARLTSRYGPGLIFTCHPNPGL
                     IGGVRVQVGSDVFDGSIQARLAELEDAF"
     gene            9162..10694
                     /gene="atpA"
                     /locus_tag="PKI07_06270"
     CDS             9162..10694
                     /gene="atpA"
                     /locus_tag="PKI07_06270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOC50695.1"
                     /translation="MSAILQEIESQISALKTAVMKQNVGMVREIGDGVAKVEGLADAM
                     YNEMLDFGGGVTGLVLNLEETEVGAIVLGDYTGIKEGQEVRTTGKVLQVPVGMELLGR
                     VVNTLGQPVDGKGPIATKMTYPVEKIAPGIIRRKSVSQPVQTGIMAIDAMIPIGRGQR
                     ELIIGDRSTGKTTLGIDTIISNARLNQAAREAGSKTYRPLYSIYVAIGQKQSNVANVI
                     RVLEEAGALPYTIIVVASASDSATNQYLAPFAGCAMGEWFMDNGMDALIVYDDLSKHA
                     VAYRQVSLVLKRPSGREAYPGDVFYLHSRLLERAARVAENHGNGSLTALPIIETQAGD
                     VSAYIPTNVISITDGQIFLETDLFYQGVRPAVSVGISVSRVGSAAQVKAMKQVAGRLK
                     GELAQFRELAAFAQFGSDLDAKTQAQIERGKRIMEIFKQPPYHPLPVEMQIAVLWVAQ
                     NGFVDNVPVERVKDFQDKLMDYLTTRRAPLLTRIRQEEALSDSLIAELKAAATEFKQT
                     YQ"
     gene            10775..11653
                     /gene="atpG"
                     /locus_tag="PKI07_06275"
     CDS             10775..11653
                     /gene="atpG"
                     /locus_tag="PKI07_06275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOC50696.1"
                     /translation="MPSTRDIRRRIKSVKSTAQITKAMQMVASSKMLKAQQGAINFRP
                     FAAMSAELMSHLTQDTDDYRHPLMETREVRRRAVVLIGTDKGLCGALNANLFREAARY
                     APATTRFIAVGKKAAQFIARTRRTLVAEFSCRDTPTYGECQAISKFAQDLFLKGEVDA
                     VDVLYTRFISTLSQQPDQRAFLPMGSALNLPAEGAASPGLRAGEPKRYHFLFEPDPAT
                     VLDRLVLYFLDFRVAQLLLEAKASEHSARMVAMKNATDNADQLIKHLTLEYNKLRQAN
                     ITKELLEISSAAMALE"
     gene            11690..13102
                     /gene="atpD"
                     /locus_tag="PKI07_06280"
     CDS             11690..13102
                     /gene="atpD"
                     /locus_tag="PKI07_06280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOC50697.1"
                     /translation="MNKGKIVQVIGPVVDVEFPAQLPAIYNALTIEYELPLQGKTRLT
                     LEVQQHLGDHWVRTVAMSTTDGLKRGMEVFDTGQPISMPVGDGVMGRVFDVTGNPVDE
                     RGPVVAEKHMPIHRSAPSLTDQSTTPQILTTGIKVIDLICPFLKGGKVGAFGGAGVGK
                     TVVIMELINNIAKLHGGVSVFAGVGERTREGNDLYREMSEAGVINQKDLSKSKIALVY
                     GQMNEPPGARLRVGLSGLAITEYFRDDKNQDVLLFIDNIFRFSQAGSEVSALLGRTPS
                     AVGYQPTLAAEMGDLQERITSTRKGSITSFQAVYVPADDLTDPAPATTFAHLDATIVL
                     DRAISELGIYPAVDPLSSTSRALAPDIVGAEHYEVARGVQKVLQRYKDLQDIIAILGM
                     DELSPEDKQTVFRARKIQRFLSQPFSVAEVFTGRPGKQVPVADTVKGFKEILDGKHDE
                     VPEGNFYMKGRIEEIREVEG"
     gene            13130..13543
                     /gene="atpC"
                     /locus_tag="PKI07_06285"
     CDS             13130..13543
                     /gene="atpC"
                     /locus_tag="PKI07_06285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOC50698.1"
                     /translation="MISEFKLEITTPTGRVYSKAVDMVTLPGREGEMGILPQHAPLIA
                     LLGDGEIVARRGDQEDRLLVTGGCVEITPERVAVITVFATPEDAIDADKAQAARARAE
                     ARLKENISPEEVALVQASLTHSLAQLKIKRRPPQR"
     gene            13907..14440
                     /gene="hoxE"
                     /locus_tag="PKI07_06290"
     CDS             13907..14440
                     /gene="hoxE"
                     /locus_tag="PKI07_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxE"
                     /protein_id="HOC50699.1"
                     /translation="MIATLSRKKKNRLPQVLEHASGDKRFKILEVHIKKHQFKQDALI
                     EVLHKAQELFGYLEDDLLLFIAYRLKLPTSRVYGVATFYHFFTLKPKGRHTCVVCMGT
                     ACYVKGADKVLAAVENKAGIKAGETTPDGELSLLTARCIGTCGIAPAVVYDGTVTPRQ
                     TPETALEHVKGWIKHGH"
     gene            14430..16139
                     /locus_tag="PKI07_06295"
     CDS             14430..16139
                     /locus_tag="PKI07_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOC50700.1"
                     /translation="MDIEDLVEIAEKERASRKKHVIRCCLAAGCMASDSKGVKEALDK
                     AVQEAGLQDQVEVRGVGCMKLCCQGPLVQVDTHESGATVQVGDDNQGPLYLKVTPESA
                     PSLIATLTGGQTSLQRGNPKAPFFTEQAPIVLANSGIVDPERIESYIAAEGYQALHDV
                     LREMTPKEVVDTMIASGLRGRGGAGYPTGLKWATVAKTPSQQKYIICNGDEGDPGAFM
                     DRSVLESDPHCVLEGMAIAAYAVGANQGFIYVRAEYPLAISRLQTAIKQAKKLGLLGS
                     GIFESPFTFNIDLRIGAGAFVCGEETALMASVEGLRGSPRPRPPFPAESGLWSWPTLI
                     NNVETFANVAPIMRKGAEWFASIGTEKCKGTKVFALAGKITNTGLIEVPMGTPLRKIV
                     EVLGGGAPDGGRIKAVQTGGPSGGCIAAEELDTPVDYDSLAKLGSIMGSGGMIVMDEA
                     TQMVDVARFFMEFCMDESCGKCVPCRTGTVQMHSLLKKILAREATARDLQKLEELCDM
                     VRHTSLCGLGQTAPNPILSTLRFFRNEYTALLKDDVHNGRNKNHPAPEPAPAPAAGPA
                     TGS"
     gene            16143..16862
                     /gene="hoxU"
                     /locus_tag="PKI07_06300"
     CDS             16143..16862
                     /gene="hoxU"
                     /locus_tag="PKI07_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HOC50701.1"
                     /translation="MAAKTLTIDGKHVSVDESATILDAAVDAGIYIPTLCHLDGVYDV
                     GACRLCLVEVAGSSKLLPACTTKVSEGMEIQTDTERLRQYRRITLEQLFAERNHVCSV
                     CVANGHCELQTLAYRQGMDHVRYDYRFPQWEVDSTHSLFGLDHNRCILCTRCVRVCWH
                     IEGAGTLNVSGRGAQARIITDLNQPWGTSDTCTKCGKCVMACPTGALFYKGATVGEME
                     RDRTKLEFIVTAREKKQWIAD"
     gene            16864..17457
                     /locus_tag="PKI07_06305"
     CDS             16864..17457
                     /locus_tag="PKI07_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOC50702.1"
                     /translation="MQADQTLETKPQSGQPARLRLATIWFGGCSGCHMSFLDLDEFLV
                     ELAGKAELVYSPVIDVKEYPQNVDVCLAEGAICNEDNRELLYQVRQRTRLLVAFGDCA
                     VTANVSALRNQLGPGNAESVLQKAYIETATANPALPGEPGIVPPLLPRVMPVHEVVAV
                     DYFLPGCPPPADRIKSFLVQLLEGQEPVLTGKDIKFG"
     gene            17462..18892
                     /locus_tag="PKI07_06310"
     CDS             17462..18892
                     /locus_tag="PKI07_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOC50703.1"
                     /translation="MAQRIVIDPVTRIEGHAKISIFLDDAGNVSDAEFHVVEFRGFEK
                     FCEGRPYAEMPGITARICGICPVSHLLASAKAGDAIMAVNIPPAADKLRRLMNLGQII
                     QSHALSFFHLSAPDFLLGWDVPPAQRNVFGLIAANADLARAGIRLRQFGQEIIAELGG
                     QKIHPAWAVPGGVRNPLTEAGRDRIRAWLPEAYATTKTAFDLFKKTLETHKKEVQIFG
                     NFPSLFMGLVEPDGTWEHHGGNLRFTDSSGSIIADQVDPQKYYECIGESVQSSSYLKS
                     PYYSPLGIPDGMYRVGPLARLNVAEQMGTPKADAELKKFKKLTRGDNAVISSFHYHYA
                     RLIEILTAIERVEATLDDADLLSEDLRADAGINSLRGVGVSEAPRGTLFHDYTVDRNG
                     LLQKINLIVATGQNNLAMNQTVAQIARHYVRGHKITEPMLNRVEHGIRCYDPCLSCST
                     HAVGQMPLHLQLIGPDGAVLHELVRG"
     gene            complement(19277..20287)
                     /locus_tag="PKI07_06315"
     CDS             complement(19277..20287)
                     /locus_tag="PKI07_06315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC50704.1"
                     /translation="MTILFDHPEPALLAHGGMLIQIKQTKAALEALGCQVDYVRWWDE
                     TQRADILHYFGRLPAYLLRIARAKGIKVVLGELLTGQGSRPAWQLWGQRQTMRLLRTL
                     APTRLTQSLRWESHRLADASVALTAWEAHLLHYLFAAPQEKIHIIPNGVEDVFLKSPP
                     AQRGQWLVCTAIITERKRVLELAQAAIQAQTPLWVIGQAFAESDAYAREFAALARAHP
                     RILRFEGPIADRAALARAYRESRGFVLLSACESLSLSALEAAACECPLLLADLPWARS
                     TFRERATYCPVPGSTRLTARALRQFYDAAPGLSLPPRPCSWLEVARQLKALYTTLQAT
                     PP"
     gene            complement(20439..20942)
                     /locus_tag="PKI07_06320"
     CDS             complement(20439..20942)
                     /locus_tag="PKI07_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC50705.1"
                     /translation="MQKLEPPDNHYLRAAEGWLELGNPVEAKAELDQVSPARQEHPDV
                     LELRWALFAAQQQWEEGLQVAQALLRSDPERSSGWLHQAYALRRVPNGGLQKAWEALL
                     PASVKFPQEAIIPFNLSCYACQLQQLDVARLWLRRAAGIGGKEQIKRLALSDPDLQPL
                     WPEIEQL"
     gene            21098..22435
                     /locus_tag="PKI07_06325"
     CDS             21098..22435
                     /locus_tag="PKI07_06325"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOC50706.1"
                     /translation="MHQVNLGMIGGGTVGSGVFHALQRNGDLLAARIGVRVTVQKVAV
                     KAVDEPRPYRIPRAVMCTDWQRVVQDPQVNLVAELAGGTTVARTMILTALKLGKPVVT
                     ANKALLSAHGEELFAAARQYGTNLYYEASVCGGIPIIKALREGFVGNRITHLYGIVNG
                     TCNYILTRMKCEGAEFADVLKDAQAQGYAEAEPSLDVDGFDAQHKIGILASLAHGFWV
                     NPRDIYVEGIRALTRADIQFAGQLGYTIKLLGIIKRIEESPARTPRGRIPSARIQVSV
                     YPALVPNAHVLASVNHVFNAAFVRGDIVGDTLFYGRGAGKDATASAVLSDLADAALDL
                     KFGTPQRVPPFVPHERAGAVVPIAEVVTRYFVRLNVVDRPGVLAQISALLGAARIGIS
                     SVIQPESREGASVPLILMLHDAPSGAVTGALKKIGRLAAVRSAPVMIRVEDLC"
     gene            22429..23643
                     /locus_tag="PKI07_06330"
     CDS             22429..23643
                     /locus_tag="PKI07_06330"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOC50707.1"
                     /translation="MLIVQKYGGSSVANTDRIKHVASRVAQCRARGDRVVVVVSAMSG
                     VTDNLIKLAREIMPLPNEREMDMLLATGEQTTIALTAIALHSLGIPAVSLTGAQAGIV
                     TDGVHTKARIQNITPRKIHELLNQGQVVIVAGFQGQTREGQVTTLGRGGSDLTAIALA
                     AAIKADLCQICTDVDGVYTADPRLVPDAKKLEEISYDEMLELASLGAKVMQSRSVEFA
                     KKFGVVFEVRSSLNDHPGTIVKEETKNMEAVVVRGVSLDKNQAKVTLVGVPDKPGVAA
                     RIFKAIGDAGVNVDMIVQNISHGSGAPATDLSFTVDKPDLPRAQKTLDALKPDIGFGR
                     ILATEKIGKLSIVGVGMKSHTGVAGRMFDTLAREGVNIDMISTSEIKISVVVDLGKAE
                     QAMRAVHAAFLG"
     gene            23722..24342
                     /locus_tag="PKI07_06335"
     CDS             23722..24342
                     /locus_tag="PKI07_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOC50708.1"
                     /translation="MSTALNQPVLVLNRLWQAVNVCSARRALTLLFQGHAQAVLSRCD
                     GSFQTFNFNQWHDFSQQEPGADAVRTVSFRIRVPRVILLLIYDRLPRKEVKFTRHNIF
                     ERDQNTCQYCGQVCDRKDLNLDHVIPRDRGGPTTWENIVCSCIECNTRKANRAPHEAG
                     MRLIRKPKRPKWRPFVQVNFTLHPHDSWKHFVDLAYWNVELGEDVR"
     gene            complement(24368..24733)
                     /locus_tag="PKI07_06340"
     CDS             complement(24368..24733)
                     /locus_tag="PKI07_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50709.1"
                     /translation="MRLFQIIFNPTSAAELARMPKELQLKILGEFRGLPQQVLGTELA
                     QFGKLERAGRMLHRFRVGDYRVYFERHKLGLVVHRILNRHTLKDFLFRSGLKVPEDEA
                     LQRNPKFWELIEAAKTAST"
     gene            25091..26320
                     /locus_tag="PKI07_06345"
     CDS             25091..26320
                     /locus_tag="PKI07_06345"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOC50710.1"
                     /translation="MKTFIKTLLILALGLPGMAPAEVKLGNEVLVEGQFKVLQGKRLG
                     LITNPSGVNRNLESTVEVLHAAPGVKLAALFGPEHGVYGDIEAGEKVDTLVDKRTGLP
                     AYSLYGKTHKPTPEMLKDLDALVYDLQDTGCRSYTYISTMGMAMEACGEAGIEFIVLD
                     RPNPLGGERIEGAMLEEKFRSGVSKWNVPYVYGLTCGELARLLNGERLIKTPCRLTVV
                     PMKGWKRSMVWSDTGLPWVPSSPHVPHGQSALYQVATGMLGELMSVSIGIGYTLPFEC
                     VASPGLDSHKLAEALNAYRLPGVKWRPITYKPYYFTFSGQPLAGAQLYFTDPARAPLT
                     AINLYAIEALKRVGGVDAFAEAERVKRGFGMFDKVNGTDATRKALQAGTPAADIVASW
                     KAGEEAFRKVRRKYLLY"
     gene            26381..26770
                     /locus_tag="PKI07_06350"
     CDS             26381..26770
                     /locus_tag="PKI07_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50711.1"
                     /translation="MRVLLLVILPGVLALSGCVGRREKAPLPPDAPRAETPATPAEPA
                     APNPPLIVTPDAAPVGKVALVNVPARHVVLTFPLQKLPPVGQRLALYRHGVAVGQVKV
                     SGPQREDNIVADLIAGEAEVGDEVRSP"
     gene            complement(26840..27919)
                     /locus_tag="PKI07_06355"
     CDS             complement(26840..27919)
                     /locus_tag="PKI07_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOC50712.1"
                     /translation="MMKAKALLAVTLGILCVGGTARAQIRNDEVYEYPNLREENYRQH
                     LVVPDIEGYKTLKCDFHIHTAFSDGKLWPDQRVLEAWNQGLDVIAITDHIEYRPHKAL
                     LNVDFNKSNEIARKKGEELGMIVISGSEITRSKPLGHLNALFIKDANKLEVPDPLDAI
                     DEALAQGAFILWNHPGWPDDDSTIYPVHRELIAKGKIHGVEVFNSFEAYPKVIDWCWE
                     LKLAPFANTDIHGIGTQLYRGRGLRPMTLVFAQEYTEASVKEALLAGRTVACYNHILS
                     GKEEYLKALIRAALSIKVINKERGMIEITNLSDINFQIQYGKLMYAVPLYPFQTLRDR
                     VPSGTKVTFTNCIKAKDQYVETTLW"
     gene            complement(27978..28316)
                     /locus_tag="PKI07_06360"
     CDS             complement(27978..28316)
                     /locus_tag="PKI07_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50713.1"
                     /translation="MTTEEFRREIAEAVKAYEKYVVCLNKTPDEFKATLKSLLGKAIR
                     AYEGRDPELRHGIALDKQVTVILSQSDSPRPLCGIYFNLHSPYQRESLPKTMKPLKES
                     VEGDPPEPEA"
BASE COUNT         5626 a         8615 c         9059 g         5603 t
ORIGIN      
        1 ggcccaacag acccaggtaa taggcacggc ggggtttcat ccggcccgcg ctgcacggtg
       61 gctgtccaaa gaccataatg tcttcatggg cctccccgac attcagccgc agccgaaccc
      121 cgtcttccgc gtcattgacg atctccagcc cggtgacggg attggggccg aagaccaccg
      181 gggcgggcgg ttcctccacc ggcgccagac ccagcccccg cagggtgctg ttgatctgaa
      241 cgcagaactg ctgcccactt aagtgcgaat actgccccat ccagggccgg ctggggacgt
      301 gcagcgccgc ggcgttccac cgctcgcgct gcgcttcgct gagcttgagg ccccactccc
      361 gcgaggagat cccaaaatac gaccgcgcgg ccgccttgcg ctccgagggt cggtcccggg
      421 gaacggtgcg ccgccgggcg cacacgccga agggggattg gtagtatgtt tccgcgccca
      481 ggctgcccgt gcgcggagag gtgagtattt tcataattaa ttacctttct gttatcgcct
      541 cggagagcga ctcggcttcc cgccactcct tggactggaa gttccagcca ccaccacccc
      601 gagggttttg gggaaggctg gcgggcggga ggtcttcgcg caagggcaat ttttcaggat
      661 tggtggtggc aggccggtgc ccgggggtca ttcgcccggc ggctgagaaa aataatcacg
      721 tccccgcccg ggttgcctgc ggccagacgg ccggaaataa cgaacaaaag gtgttgatgc
      781 cgcagccggc tctcgctaaa atcggcacat gcttggtgag ccccaaaacc aatttcccct
      841 cccttgcgca gcgctgagta cgcgcggcac gcaaaagcaa tttcccctcc ttatccgatt
      901 tatgaaaagg caactccgaa agataagaca acagttgggt agcgcggctg gatggggcct
      961 ctgcttgctg gcgttttccg ccggcgccga aatcaatctc acctccacga cgctggcgat
     1021 ttcgggcgat tggcaggaaa cctggtacct ggaagcggat catcaggagc accataatga
     1081 cgggactttt ggcgattcga cgcagagcga accgagcctg agtctttcgg tctcctcgtc
     1141 cccttacggc tgggcagggg cgtcgggcat attaagcgcc tttggcttgc gcctggaagc
     1201 ggacgccgcg cctcgaggcc tctcttatga gtgggggatc ggcagtcttg agcccacgac
     1261 gatcaacacc tatgcgacgg tcgccacccg gtttcaagtg gagcagccgg ctttggaaat
     1321 ccagtggtca ggatgggcgg cgtttaatta cgcaatgaat gaacaagaca tttacctgga
     1381 gttgagggat gccacgacgg cgacgtcact gctgcagtgg caagcttccg ccgattggga
     1441 tacgtggggg agtggaggat ttgccacggt ttatcccctg acggtgacgc cggctcatga
     1501 atatgaattg atcctggcgg gctggattac cgcctgggac ggcaagtacg cggtgctgga
     1561 ggctgacgtg atgatccgcg aggttccgga accggccagc ggatggctct tactcctggg
     1621 ctggggtctg gcgctggccg gacggagagg gcggtgaaga atggaggctg gagaatggag
     1681 gagggaagcg ggcaagttgg cagccgcaag tgtgtggctg cgggttaaac gagctcattg
     1741 gcgttccccc agagcatgga tttcacgaaa accgcgcatc ttcctgaagg atacggcggg
     1801 ttgatgcgct cgtgagcgta aagttttaag ttgaacggca tttagatggc ggtcggctgt
     1861 tccataggtt cagttcagac ccggcaaata ggtttgcagg gatgttccca gaaagcgccc
     1921 gccgtctccg gctgtcccat agacagccaa caagctgcgc agttcgagct tttgcccaga
     1981 ggccgaacgc accagcgcaa agagccgggt aaagcggtga gcccactggc tggggaaggc
     2041 gcggaagcgc agccgcaggt agacccgcag cgccgtccat acctgccagc gctcggcatt
     2101 ggcgttatga cccgggaagt cggcccattg gaggctctgt ttgagttcct tgaagaaggc
     2161 ctcgatgctc cagcggcagc gatacagatc ggcaatgatt tgggtgcttc attcccaatt
     2221 gttggtcagt aagaccacct cccgcatctg cccggcggcc tccgcccaag ccactacgcg
     2281 tcgcatcccg ttggggtaaa cttggtggga aaaggcgggg agtatttgcg caaggtcaat
     2341 tttggaggat tggtggtggc ggatcggggc ggagcgccgc gtgccctctc gtggcgcatg
     2401 agtgccattt gcccagtagc tgagaaaaac ctccacgcac ccgctcgagc tgcctgcgac
     2461 caggcgggcg caaataacgg ggtgacaaaa ggcgttgaca tcgtaaccga ttctcgctag
     2521 acttgcgtta tgcttggtga gtcccggaac caattctcac tcacccgcgc ggtgctgagt
     2581 aagcgcggca cgcaaaagca aatttctatc aatagtttag ttagaatcag tattagttta
     2641 tgattgaaat ttggaacagc cttgttaacc aagtcaccga ctacgctccc aacctgttag
     2701 ccgccttggc ggtgttggtg ggcggctgga ttgtcgccct cttaatttcg gcgttactac
     2761 gcaaactcct ggtgcgcacc gcaattgtta acaaactcac caaatggatc gccggggccg
     2821 agagcaagcc cgtcccgatc gagaaatgga tcggcacctt ggtcttctgg ctgattatgc
     2881 tggtggtatt ggtggccttt tttcagatcc tcaagttaac cgccgtcact gaaccgttgc
     2941 agagattggt ggataatttc actcaatatc taccgcatct ggcggcggga ttggcgttag
     3001 cgttgcttgc ctggttggta gcggtgttgg tgcggcgggt gctttccgac gggttgaagg
     3061 cgacggaatt tgaccagcgg ctggggcgca ttgccgcgtc agatcaacgc cctgatcaat
     3121 ccgtgagcaa gcctattgct gacacggtat actgggcggt attggtggcc ttcctgccgg
     3181 cgattttagg tgctttacga atgaacgcgt tgctggaacc ggtcaacgcc atgctcagca
     3241 agtttttcgc ttttgtgcca aacttggcgg gcgcattggt aatcctgttg gtgggttggt
     3301 tcgtggcgcg gctggtgcag agactcgcgg ccagtttgtt ggccagcgcc ggagcggacc
     3361 gtttggccga aaagtggggc ttcgccaagg cgctcggaac gcgacgtttt tccgaagtca
     3421 ttggcctggt cttgcagatt gtcattttgg tgccggtatt gattgcggct ttggatcagt
     3481 tgcacatgga agccgttacc cgtccggcca gcgacatgtt gcaggcgatg ctgattaaag
     3541 tgccgcagat cgtggcggcc agtttgatct tgttggttgc gtggtttatt ggccgggtgt
     3601 tggcgggtct ggttgagaac cttttgggtg gtttgggatt caatcaaatc ctcgttaaac
     3661 tcggcctttc caaaaaagcg caagacggtc gggctgctcc cgccgccatc gtgggcgcgt
     3721 tactgattat tgggatcatg cttttggcgg cgattgcggc agcggaaacg ttgggctaca
     3781 cgcgaattgc cggagtggtg gagaattttc tggagtttgc cggcaaggtg ttgctgggag
     3841 tgttgctttt cggtttgggg ctgttgctgg ccaatatggt ctttaacgtg atcaatgcca
     3901 gcgactcacc ccgtgcgcga ggattggcct ggaccgcgcg tattgccatc atcattctgg
     3961 tcggcgcgat gtcgttgcgg gcgacggggg tagcggactc aatcgtgaac cttgcttttg
     4021 gtgcgctggt ctgcggggcc gcggctgcgg gtgcgatcgc ttttggtttg ggcgggcgcg
     4081 acttcgcttc caggcaattg gcccgatggc aaaaccatac gaacgaaaag catacataat
     4141 tgagcaactg cgtgcgttgc gggtgcggtc cgccgtaaat ttgagcatgc cgagcggatg
     4201 tggtgatgca cgggtctaga ttggcgttga agcggaagta gtctacgcaa ccgacatgcg
     4261 gttcgcgcat cgtgccttcc agttcgggag gatctgcttg gcatggatgt ttcacccttg
     4321 cagcgctgcg atgcggcttt ggaagccggt gggatgcgga caagtggggg aacgcgcatc
     4381 aatgccaaag cgcggttggt accgcaggcg gttgaagaag tgggggcaca agaaagttta
     4441 ggattgatta ttgcaagggg ccgttcgtaa ggatggcttt tgggcagttt ataagctgag
     4501 acaagatgac tcataatatc agacgggtgc ttttcccaat tccttatctt tcggaactca
     4561 aaaccaaaac gatgaaaatt cgctcacact acctcggcct ggttctggtt gtttgtttca
     4621 ccggccggat tgccgcggcg cagacaatag acgaaaaatt ggaagcgatg atgaaagagc
     4681 aacctactgt tgggctctcc gtcgcggcag taaaagatgg caagcttatc tacacccatt
     4741 ccttcgggta taagaatctg gaaaccaaaa caccgctttc agataacgac atttttcgca
     4801 tagcctccat ctccaaatca ttcacttccg tcgggctgat gcaaatgatt cagaagaaga
     4861 aaatcagttt ggacgatgat gtaagcgccc tggcgggatt cagaatccgc aatcccaggt
     4921 tcccggataa ggtcatcacc ctgcggatgg tgctgtcgca cacttcgagc attaacgacc
     4981 gccagggata ttttgatctg gttgcgataa accccgataa gaatccggat tgggcaaagt
     5041 gctacaacga ttacgaaccg gggagtgctt atgaatattg caactataac tacaacattg
     5101 ccggcgccat cctggagcgt gtatcgggag aaagatttga tcactacatt gccggccaca
     5161 tcctcaagcc gttaggcatc tccggcggat tcaatgtcaa cgatctcgac ccctcgcgct
     5221 ttgcgttgat ttacgattat aacagggaga cggcgaggtt tattctctct ccggccgcct
     5281 atgcccctcg taaaaaagag atttcagagt atgaaatggg ttttaccact ccggtctttt
     5341 cacccgcggg ggggctgaaa atatcagcag gcgatctggc ccaatacatg atgatgcaca
     5401 tgaaaaacgg gaagcaaaac gggaagcgga ttatcgcaaa aaagaacgcc aaactgatac
     5461 ggacaccgct ttccgcgaaa gagcattacg ggctcggact gttgctgaat cccgatctca
     5521 tccccggcgt caatatgtac gggcatacgg gcggggctta cgggctgacc agcctgatgt
     5581 tcttcgagcc ggagaagaaa ttcggatttg tggccatttc caacggaaac gccccgtcct
     5641 actcttccgg cagaaaggcc attaaaatca tgtatgagga attcatccgc gcgcgcgctg
     5701 attaggcgcg gcgcgacaaa cgcaaatccc tggcagcagc agccgggagg cgtggagcct
     5761 atttgcggac ggattcgatg tgcttgccgc gcaccgtttg ggcgcggaaa tcccacagca
     5821 gccagtcgtc atcgggcaca aaccggtcga gtttgccgtc ccacttcctg ggcagccgcc
     5881 ggttgaggcg ttcgaggaac ggcagcagcg ggcgctgggg gggaaaccgc cgttcgtggt
     5941 ggtcaatcgg aatgtccacg ttccagccaa agcgtttgcg ataggccgca aaaccaaagc
     6001 cgtcgaagaa gttttctccg ccaaccagcg ggagatacca gaggctgggg atgccgcggg
     6061 gattttcctt gatggggatt tcctgttcca gccgcgccag gagcgccagg ccggccgggt
     6121 cgtccgccag gtgcgcgcgc aattcatgca gggcggggaa ttggattggg gggctgacaa
     6181 agagcaccgg tttgtgctgc atggtggcca ggatgatttc gtgcggcgtg ccgacgctat
     6241 agatgttggt cgggcaatac gagatgctga aatcgctggt atccaccatc cgcaggtcaa
     6301 tgtgcagcgt ctcccagaat tgccgggcgc accacgcgcg ggcctgcgcg ccgcgcttgc
     6361 cgccggcata agtccaccgc cggcggatgc gctcgccgct cttaaggtcc tcccggccat
     6421 attcgtgcaa cccccggacg tccggcttga accagggatc gaagacggtg acccccatgc
     6481 cctggaggaa ctggctgacc cggttgcgcc agcccgattg cttttccgcc gcgcgggacg
     6541 ccacaaaatc catcggcccg gaaagataga cccgcgctcc gcgcaataaa tcgcctttgc
     6601 ctgttgaggt cggcattgcg gacatttaag ggtgagaggc ggcgcggctc aagcctcttc
     6661 gggcggcgcc ggcgccggcg gggccgcttt cccgcaggct ccggcgcttg cgcccgcggg
     6721 gagatctttc cgccggccgg ggcgcgggcc gggcatcgtg ttgctgacgg ggaggcgaag
     6781 atttcttgcc aagcgcgccg tagtgcccca tgttttcctg acttccggcc tggcgccggc
     6841 ttaagcccgg ggggctcgcg catcgtagcg cccgggggtt caacgccatg tgcttgcata
     6901 gagtgatttt tatatcgtgg tggcttgcgg ttctgttcgg gaccggcctg gccggcgcgg
     6961 cgtcgtcggc attggcggcg gacgcggcgg ccgtctccgc ggcggcggcg cccgcgctgg
     7021 aagaggggtt gtcccccgac gccccccggc tcggcccggg cggggcctcg tttatcaaca
     7081 gctcgatggt ggcgacgtgg ggggtggcgc tgatgcttat cctgctggtg cggcgggcca
     7141 cgcgccgcat gcaactcgcc ccggaggggg cgcagaactt ctgggaatgg ctggtggaaa
     7201 tgctgcatga ttttctggag ggcattattg gcggcaagct ggtgaagaag acgttttggt
     7261 tctttgccac gctgtttatc ttcatcctgt tcacgaactg gtttggcctc ctgccggggg
     7321 tgggcagcat tggctggggg acgcagacgc cggaggggtt tcacgtcgag cgcccgctgc
     7381 tgcgcggcgg caatgccgat ttgaatatga ccttcgcgat ggcgatgatt ttctttgtct
     7441 gctggattgt ctgggcggtg caggcggtgg gggtcaaagg ggtgtttctt gatttgtttg
     7501 gccccaaggg cgataccgcg ggggccttga agtatttgat gatcgtggtg tttttcctgg
     7561 tcggatttct ggaagtgctg tccatcctgt tccggcccat ctcgctgagt tttcgtctct
     7621 acggcaacat ttttgccggg gagaacatgc tcgaggcgat gggcaagctg gtgccgggcc
     7681 tgggctggct gctgcccgtg ccgttctatt ttatggaact gctggtgggg atcgtgcagg
     7741 ccctggtgtt catgctgctg acggccgtgt tcacgatgct gatttgcgtg catgaggaaa
     7801 gcccgggagg aaccgggcac tgagagacca tttcggcggc cggaccgtgt tcactcgcgg
     7861 gggccgcgga acaacagaaa ggtaaagcga actatgttaa ctccaatgtt ggcagaggga
     7921 ctggcgggga atctgcacgt cggcctggcg gcgctcggcg cggccatcgg cgtgggattc
     7981 atcggcatga aggcctccga agcggtggga cgcaatcccg gcgcggcgac caaaattctg
     8041 gtgcaggcga tcctggcgat tgcctttgcg gaagccatcg tgttctacgc catcttcctt
     8101 gtgaagtaag ctccggccgc ggagcccgcg cggctccgcg tccggccttt gccgtgtttg
     8161 attatgcagc cgtcttttct ctttgccagc atcggcagcg acctgggcca gatggcgcgg
     8221 gacaccgcca cgcggtttgg cgtggatctg ccgcattttc tcgcgcagtg cctttgcttc
     8281 gccattgtgg cttttgtgct ccagcgctac gcctacaagc ccatcctgac cgtgctggaa
     8341 gaacgcaaac gccgcatcaa ggaaagcctt gaccaggccg agaagatcaa gcaggagctg
     8401 gcccacacgc agacccggac gcaggaaatg ctgctccagg ccggccagca ggccgcccag
     8461 ttgattgagg aagcgcgcgc cgccgccgcc aaagtgcagg aacaggaaac ccagaaagcc
     8521 atcgccgccg cccaggccat cgtggacaag gcccgccagg ccacccaggc cgagcgcgag
     8581 cgcatgttgc tcgaactgcg ccgtgaagtt ggccgcctgg tggtcgccac cacggccagg
     8641 gtggccggca aagtcctgac cgcggaagac cagagccgcc tggtcgagga aaccaaccgc
     8701 gaactggcgg cctgatgccg ccgccgcccc gcgtttagcc cgtgaaaatc tccaaacaag
     8761 cccgccgcga cgcccgggaa ctgttccgcg cctgctgccc ggacgggttg ctggacgagg
     8821 cccgggtgcg ccaggccctg cggcaggtgc tcgccacccg cccccggggc tacctggcca
     8881 tcctgtccca tttccgccgc ctggtgaagc tggacctcga ccgccggacc gcccgcatcg
     8941 aaagcgcgac gccgctgagc gccgcgcaac aggcggccct ccaggcccgc ctgacgagcc
     9001 gctacggccc ggggctgatc ttcacctgcc atccgaaccc cgggctgatc ggcggcgtgc
     9061 gggtgcaggt gggcagcgac gtctttgacg gcagcatcca ggcccggctg gccgagttgg
     9121 aagacgcctt ttaattccct ttgtaattcg accacacagt tatgagtgca attttgcagg
     9181 aaatcgaaag ccagatcagc gccctgaaaa ccgcggtgat gaagcagaac gtgggcatgg
     9241 tccgtgaaat cggcgacggc gtggccaaag tcgagggcct cgccgacgcg atgtataatg
     9301 aaatgctgga tttcggcggc ggtgtcaccg gcctggtcct gaacctggag gaaaccgagg
     9361 tcggggccat cgtgctgggc gactacaccg gcatcaaaga aggccaggaa gtgcgcacga
     9421 ccggcaaggt gctgcaggtg ccggtgggca tggaattgct ggggcgggtg gtcaacaccc
     9481 tgggccagcc ggtggatggc aaggggccga tcgccacgaa aatgacctac ccggtggaga
     9541 agattgcccc cggcatcatc cggcgcaagt cggtcagcca gccggtccag acgggcatca
     9601 tggccataga cgccatgatc cccattggcc gcggccagcg cgagttgatc attggcgacc
     9661 gctcgacggg caagaccacg ctgggcattg ataccatcat cagcaacgcc cggctcaacc
     9721 aggccgcccg ggaggccggg agcaaaacct accgccccct gtattcgatc tacgtggcca
     9781 tcggccagaa gcaatccaac gtcgccaacg tcatccgggt cctcgaagag gcgggggcct
     9841 tgccctacac catcatcgtg gtcgcgtccg cctcggactc cgccaccaac caatacctcg
     9901 cgccctttgc cgggtgcgcg atgggcgaat ggtttatgga caacggcatg gacgcgttga
     9961 ttgtctatga tgatttgtcc aagcacgccg tggcctaccg gcaggtctcg ctcgtgctca
    10021 agcgcccctc cgggcgcgag gcgtatccgg gcgacgtttt ctacctgcac agccggttgc
    10081 tggagcgggc cgcgcgcgtg gcggagaatc acggcaacgg atcgctcacg gcgcttccga
    10141 tcatcgaaac ccaggcgggc gacgtttcgg cgtatatccc caccaacgtc atctcgatca
    10201 cggacggcca gattttcctg gaaaccgact tgttctacca gggtgtccgc ccggcggttt
    10261 cggtcggcat ctcggtctcg cgcgttggtt cggcggcgca ggtcaaagcg atgaagcaag
    10321 tggccggacg cctcaagggg gagctggctc aattccggga actggcggcg tttgcccagt
    10381 tcggctccga cctggacgcc aagacccagg cgcagatcga gcgcggcaag cgcatcatgg
    10441 aaatcttcaa acagccgccc taccacccgc tcccggtgga gatgcagatt gcggttctgt
    10501 gggtcgccca gaatgggttt gtggataatg tgccagtcga gagggtcaag gatttccagg
    10561 ataaactgat ggattatctc accacccggc gggcgcccct gctgacccgg attcggcagg
    10621 aagaggcgct cagcgattcc ttaatcgccg aattgaaagc ggcggccacc gagttcaaac
    10681 agacttacca gtgaacccgc gcctgacctc gcaacgcccg atggctgccg ccggttttcc
    10741 ggttcctggc cgggaacccg cggagccttg acccatgccc agcacgcgcg acatccgccg
    10801 ccggattaaa tcggtcaaaa gcacggccca gattaccaag gccatgcaga tggtggcctc
    10861 gtcgaaaatg ctcaaggccc agcagggcgc gattaatttt cgcccgttcg ccgccatgag
    10921 cgccgagttg atgagccact tgacgcagga cacggacgat tatcggcatc cgttgatgga
    10981 gacgcgcgag gtgcgccggc gcgcggtggt tctcattggc accgacaagg ggctttgcgg
    11041 tgcgctgaac gcgaacctgt ttcgcgaggc ggcccggtac gcccccgcca ccacccggtt
    11101 catcgcggtc ggcaaaaagg ccgcgcagtt catcgcccgc acccgccgca cgctggtggc
    11161 ggagttttcc tgccgggaca cgccgacgta cggcgagtgc caggccatct cgaagttcgc
    11221 ccaggacctg tttctcaaag gcgaggtgga cgcggtggac gtgctttaca cccgcttcat
    11281 cagcacgctt tcccaacagc cggaccagcg cgccttcctg cccatgggct ccgccctcaa
    11341 cctcccggcg gaaggagccg cctccccggg gcttcgagcg ggggaaccca aacgctacca
    11401 ctttctgttc gagccggacc cggcgacggt gctggaccgg ctggtgcttt acttcctgga
    11461 ttttcgcgtg gcacagttgc tgctcgaggc caaagccagt gaacacagcg cccgcatggt
    11521 ggccatgaaa aacgccaccg acaacgccga ccagctaatc aagcacctga cgctcgaata
    11581 caacaagctg cgccaggcca acatcacgaa agaattgctg gaaatctcca gcgccgccat
    11641 ggccctcgag taggcgcgcc cgtcattcct tacgcatcat tccaaacgca tgaataaagg
    11701 caaaatcgtt caagtcatcg gcccggtggt ggatgtcgag ttcccggctc agctccccgc
    11761 catctacaac gccctgacca tcgaatacga gcttccgctc cagggcaaaa cccggctgac
    11821 cctcgaagtc cagcagcacc tgggcgatca ctgggtgcgc accgtcgcca tgtccaccac
    11881 ggacgggctt aaacgcggca tggaagtatt cgataccggc cagcccatct ccatgccggt
    11941 gggcgacggc gtcatggggc gcgtctttga cgtcaccggc aacccggtgg acgagcgggg
    12001 gccggtggtg gcggaaaaac acatgccgat tcaccggtcg gccccgagcc tgaccgacca
    12061 gtccaccacc ccgcaaatcc tcaccaccgg catcaaggta attgatctca tctgcccctt
    12121 cctcaagggc ggcaaggtcg gggcgtttgg cggcgccggc gtgggcaaaa cggttgtcat
    12181 catggagttg atcaacaaca tcgccaagct gcatggcggc gtgtcggtgt tcgccggggt
    12241 cggtgagcgc acccgcgaag gcaacgacct ttaccgcgaa atgtccgaag ccggcgtcat
    12301 caaccagaag gatctcagca aatccaagat cgccctggtc tatgggcaga tgaacgagcc
    12361 gccaggcgcc cgcctgcggg tgggcctgtc cggcctggcc atcacggagt atttccgcga
    12421 tgacaagaac caggacgtgc tgctgtttat tgataacatc ttccggttct cgcaggccgg
    12481 ttccgaggtg tccgcgctgc tcgggcgcac gcccagcgct gtcggctacc agccgacgct
    12541 ggccgccgaa atgggcgacc tccaggagcg catcacctcc accaggaaag gctccatcac
    12601 cagttttcag gcggtctatg tgccggccga cgacctcacc gaccccgccc ccgccaccac
    12661 cttcgcgcac ctggacgcga ccatcgtgct ggaccgcgcc atctccgaac tgggcatcta
    12721 cccggctgtg gacccgctgt ccagcacctc ccgcgcgctc gcgcccgaca tcgtcggggc
    12781 ggaacattac gaagtcgccc ggggcgttca gaaggtcctc cagcgctaca aagacctcca
    12841 ggacattatc gccatcctgg gcatggacga attgtcgccc gaggacaagc agaccgtgtt
    12901 tcgggcgcgc aagattcaac gcttcctcag ccagcccttt tcggtggcgg aagtgttcac
    12961 cggccggccc ggcaaacagg tgccggtggc ggatacggtc aaaggcttca aggaaatcct
    13021 cgatggcaag catgacgagg ttcccgaggg caatttctat atgaagggcc gcatcgaaga
    13081 gattcgggaa gtggaaggtt gaggagaaac catcgccgcg caccgcgcca tgatttcgga
    13141 gttcaaactg gaaataacca cccccaccgg ccgggtttat tccaaggccg tggacatggt
    13201 gacgctcccg gggcgggaag gcgagatggg catcctgccg cagcatgcgc ccctgatcgc
    13261 tttgctgggc gatggtgaaa tcgtcgcccg gcggggggac caggaagacc gtctgctggt
    13321 cacgggcggg tgcgttgaaa ttacgccgga acgcgtggcc gtgatcaccg tctttgcgac
    13381 ccccgaagac gccattgatg ctgacaaggc ccaagcggcc cgcgcccgcg ccgaggcccg
    13441 cctcaaggag aatatttccc ccgaagaagt cgccctggtc caggcctcgc tgactcattc
    13501 gttggcccag cttaagataa agcgccgccc tccgcagcgt tagccggcca ttgcggcggt
    13561 ccgcacggag ggaagcggga gtggcttgcg cgctcccggc ccggcggcgg ggccagggga
    13621 gaggaacagg gctatggaac cgttggcgag ggcagggctt cccgcgattc ctggccggcg
    13681 cgccgggttg cggccgcctg cgcgccggtt tttcgcgatc ttttcccgcc agccattgag
    13741 ctgtgccagc gggttcgggg ccatttccct gcacaaaaaa ggtttatcaa aagccaatcc
    13801 aggaacagcg aattggcccg ccagtgctac tttgtggcag gaattgagcg ggactgcggc
    13861 gctggaaaag accgtgccgg gcccctcctc agtccggttt agcgatatga tagcaacgct
    13921 ctcacgcaaa aagaagaatc ggctgcccca ggtcctggag cacgccagcg gcgataagcg
    13981 cttcaagatt ctggaagtgc acatcaagaa gcatcagttc aaacaggatg cgctgatcga
    14041 ggtgctgcat aaggcgcagg aactgttcgg ctacctggaa gacgacctgc tgctgttcat
    14101 tgcctatcgg ttgaagctgc cgaccagccg cgtttatggc gtcgccacct tttatcattt
    14161 cttcacgctc aagcccaaag gccggcatac ttgcgtcgtc tgcatgggca ccgcctgcta
    14221 tgtcaaaggc gcggacaagg tgctggcggc cgtggagaac aaggcgggga tcaaggccgg
    14281 cgaaaccacc ccggacgggg agttatcgct gctgacggcg cgctgcatcg gcacctgcgg
    14341 catcgccccg gcggtggttt atgatggcac ggtcacgccg cgccagaccc ccgagacggc
    14401 attggaacat gtgaaaggat ggatcaagca tggacattga ggatctagtc gagatcgcag
    14461 agaaagagcg tgcgtcgcgg aagaagcacg tcatccgctg ctgcctggcg gcgggctgca
    14521 tggcgtccga ctcgaaaggc gtcaaggaag ctctggacaa agcggtccag gaggcggggc
    14581 ttcaggacca ggtcgaggtg cgcggggttg gctgcatgaa gttgtgctgc cagggaccgc
    14641 tggtgcaggt ggatacccac gaatcggggg ccacagtcca ggtcggcgac gataaccagg
    14701 ggccgcttta tcttaaagtc accccggaaa gcgcgccgtc attgatcgcc acgcttacgg
    14761 ggggacaaac ttccctgcag cgcggcaatc cgaaggcgcc gttcttcacc gagcaagcgc
    14821 ccatcgtgct ggccaatagc ggcatcgtgg accccgagcg cattgaatcc tacatagcgg
    14881 cggagggcta ccaggcgctg cacgacgtgc tgcgtgagat gacgcccaag gaagtggtgg
    14941 acacgatgat cgcgagcggg ctgcgcggcc gcggcggcgc gggctatccc accggcctta
    15001 aatgggccac ggtcgccaag acccccagcc agcagaagta cattatttgc aacggcgacg
    15061 aaggcgaccc cggcgccttc atggatcgca gcgtgctgga gagcgacccg cactgcgtgc
    15121 tggagggcat ggccatcgcc gcctacgcgg ttggcgccaa ccagggtttt atctacgttc
    15181 gcgccgagta tccgctggcc atcagccgcc tccaaaccgc cattaagcag gccaaaaagc
    15241 ttgggctgct gggcagcggc atctttgaat cgccgtttac gttcaacatt gacctgcgca
    15301 ttggcgccgg cgcgttcgtc tgcggcgagg aaacggcgct catggcctcg gtcgaggggt
    15361 tgcgcggttc gccccgcccc cgcccgcctt ttccggcgga aagcggtttg tggagctggc
    15421 cgactctgat caacaacgtc gagacctttg cgaatgtggc cccgatcatg cgcaaaggcg
    15481 ccgagtggtt tgcgagcatc ggcacggaaa agtgcaaggg cacgaaagtg ttcgcgctgg
    15541 cgggcaaaat caccaacacc ggcctgattg aagtgccgat gggcaccccg ctgcggaaga
    15601 tcgtcgaagt cctgggcgga ggcgcgcccg atggcggcag gatcaaggcc gtgcagaccg
    15661 gcggtccttc cggcggctgc attgcggccg aggaactcga cacgccggtg gattacgatt
    15721 cgctggccaa actgggttct atcatgggct cgggcggcat gatcgtaatg gacgaggcca
    15781 cccaaatggt ggatgtggcc cgcttcttca tggaattctg catggatgaa tcgtgcggga
    15841 aatgcgtccc ctgccgaacc ggcacggtcc agatgcacag ccttctgaaa aagatcctgg
    15901 cccgcgaagc caccgcgcgc gacctgcaga aactcgaaga actctgcgat atggtgcgcc
    15961 acaccagttt gtgcggcttg ggccagaccg cacccaatcc gatcttgagc accctgcggt
    16021 tcttccgcaa tgaatacacg gccctgctca aggatgatgt gcacaacggc cgcaacaaga
    16081 accacccggc cccggaaccg gcgcccgccc cggccgccgg ccccgccacc ggaagctgat
    16141 ttatggctgc caagacctta acgattgatg ggaaacacgt aagcgtggat gaaagcgcga
    16201 ccatcctgga cgccgctgtg gatgccggca tctacattcc aacgctctgc caccttgacg
    16261 gggtgtatga cgtcggcgcc tgccggcttt gcctggtgga ggtcgcgggc agttccaaac
    16321 tgctgcccgc ctgcaccacc aaggtgagcg agggcatgga aatccagacc gacaccgagc
    16381 ggttgcgcca ataccgccgg ataacgctgg agcagctctt tgccgagcga aatcacgtct
    16441 gctcggtttg cgtggccaac ggccattgtg aactgcagac gctggcctac cgccagggca
    16501 tggatcatgt gcgctacgat tatcggttcc cgcaatggga agtggacagc acccattcgt
    16561 tgtttggcct ggaccacaat cggtgcatcc tttgcacccg ttgcgtgcgc gtctgctggc
    16621 atattgaggg cgcaggcacg ctgaatgtct ccgggcgcgg ggcccaggcg cgcattatca
    16681 ccgacctcaa ccagccttgg ggcacgtctg acacctgcac caagtgcggc aaatgcgtca
    16741 tggcctgccc caccggcgcg ctgttctaca agggggcaac cgtgggcgaa atggaacgcg
    16801 accgcacgaa gctcgaattc atcgtcacgg cgcgggagaa aaagcagtgg attgcggact
    16861 agcatgcaag cagaccaaac tttagaaacc aagcctcaat ccggccagcc ggcgcggctg
    16921 cgcctggcca ccatctggtt cgggggttgc tcgggctgcc atatgtcgtt cctggacctt
    16981 gatgagtttc tggtggaact ggcgggcaaa gccgaactcg tttacagccc ggtcattgac
    17041 gtgaaggaat acccccagaa cgtggatgta tgcctggccg agggcgctat ttgcaacgag
    17101 gacaaccgcg agctgcttta tcaggttcgc cagcgcaccc ggctgctcgt tgccttcggc
    17161 gactgcgccg tcacggccaa cgtatccgcc ttgcgcaacc agctcggccc aggcaacgcg
    17221 gaaagcgtcc tgcaaaaggc ctacatcgaa acggcgacgg ccaatcccgc cctgcccggg
    17281 gaaccaggca ttgtgccgcc gctcctgcct cgggtaatgc cggtgcacga agtcgtggcg
    17341 gtggactatt tcctgccggg ctgcccgccg ccggcagacc gcatcaagtc ctttctggtg
    17401 cagctcctcg aaggccagga gccggtgctt acgggcaaag atattaaatt tggatgagat
    17461 tatggctcaa cgcatcgtca ttgaccccgt gacccgtatc gagggtcacg ccaagatcag
    17521 catctttctg gacgacgccg gcaatgtctc ggacgccgag ttccacgtcg tcgagttccg
    17581 cggcttcgag aagttttgcg agggccgccc ttacgcggaa atgcccggca tcacggcgcg
    17641 catctgcggc atttgtccgg tcagccattt gctggcctcc gccaaggccg gggatgccat
    17701 catggccgtt aacattcctc cggcggcgga taaactgcgc cgcctcatga acctcggcca
    17761 gatcatccag agccatgcgc tgagcttctt tcaccttagt gcgccggact tcctgctggg
    17821 ctgggacgtg ccgcctgccc agcgaaacgt ctttggattg attgcggcta acgccgacct
    17881 ggcgcgggcc ggcatccgcc tgcgtcagtt tggccaggag atcatcgcgg agcttggcgg
    17941 ccagaaaatt cacccggcct gggctgtgcc gggcggcgtg cgcaaccctc tcaccgaggc
    18001 gggtcgcgac cgcattcgcg cctggctgcc ggaagcctac gccaccacca aaaccgcctt
    18061 tgacctgttc aagaaaacac tggaaaccca taagaaggaa gtccagatct tcggcaactt
    18121 cccctcgctc ttcatgggat tggtcgaacc ggacggcacc tgggaacacc acggcggcaa
    18181 cctccggttc accgacagca gtggcagcat cattgccgac caggttgatc cgcagaagta
    18241 ctacgagtgc atcggcgaat cagtgcagtc gtcctcctat ctcaaatcgc cgtattacag
    18301 ccctcttggc atcccggatg gcatgtatcg cgtcggcccg ctggcccggc tcaacgtcgc
    18361 cgagcaaatg ggcacgccca aagctgacgc cgaactgaag aagttcaaaa agctgacgcg
    18421 cggtgataat gccgtcatct cctccttcca ttatcactac gcgcggctca ttgaaatcct
    18481 taccgccatc gagcgagtgg aagcaaccct ggacgacgcc gacctgctca gcgaagacct
    18541 ccgcgcggac gccggcatca atagtctccg tggtgtgggc gtcagcgaag ctccgcgcgg
    18601 caccctgttc cacgattata ccgtggaccg caacggtctg ctccagaaaa ttaatctcat
    18661 tgttgccact gggcagaata acctggccat gaaccagacc gttgcccaga tcgcgcgcca
    18721 ctacgtgcgc ggccacaaaa tcaccgagcc gatgctcaat cgcgtcgagc acggcatccg
    18781 ctgctacgat ccctgcctga gttgctctac ccacgctgtg ggccaaatgc ctctgcatct
    18841 ccaactcatc gggcctgacg gcgccgttct gcacgaactc gtgcgcggtt gacctgggat
    18901 tgcttttggc aaaacgcttg atagggccga accagcccgg ctcctgatcc atttgccagg
    18961 aataggcttg ggctttcatc gagagagagc gggaggcaga tgagcagacc tccctgagcg
    19021 aagcggcgag aacttccgac tggcttcgga ggcgtccccg gaacggtagg gcaccacagc
    19081 cgatgccgtc cacgcagatt gaccgtttcc gccccgcggg aactttacgg cccccaatcg
    19141 tgttgaataa tggccttgga agcccgccca atgtcggaac cgcattcttc gatcaaccaa
    19201 ctgaagcaca cgggttttcg gactgggcgt gagtttctgc tgccgagtgt agaacggctg
    19261 ccgtggttgc ggcggtttat ggtggcgttg cctggagtgt tgtgtatagc gccttgagtt
    19321 gccgggccac ctccagccat gagcatggcc ggggcggaag ggacaatcct ggcgctgcgt
    19381 cgtagaactg gcgcaaagcc cgggccgtga gccgtgtgga gccaggcacg gggcagtagg
    19441 tggcgcgctc tcggaaagtg gacctggccc aaggcagatc tgccaggagt aaagggcatt
    19501 cgcacgccgc ggcttccagc gccgagagac tgaggctttc gcaggcgctc aacaagacga
    19561 agccccggct ttcgcggtag gcgcgcgcca gagcggcgcg atcagcaatc ggcccctcga
    19621 accgcaggat ccgcggatgc gcccgggcca gcgccgcaaa ctcccgtgca tacgcatccg
    19681 attcggcaaa tgcctggccg ataacccaca gcggcgtctg ggcctggatc gccgcttggg
    19741 ccaactccag cacgcgcttg cgctcggtaa tgatggcggt gcaaacaagc cactgtcctc
    19801 gctgagccgg ggggctcttc agaaagacgt cttcaacgcc gttgggaatg atgtgaattt
    19861 tttcctgcgg cgccgcgaac aggtagtgca gcaagtgtgc ttcccaggcc gtcagcgcca
    19921 cactggcatc agctagccga tggctttccc agcgaaggga ttgggtcaat cgcgtcggcg
    19981 ccagggtgcg cagcaggcgc atggtttggc gttggcccca caactgccag gccggccgcg
    20041 agccttgccc ggtcaacagc tctcccagga caactttgat gcccttggcc cgcgcaatgc
    20101 gcagcagata ggcgggcagg cgcccgaagt agtgcagaat gtcggcgcgc tgggtttcat
    20161 cccaccagcg cacataatcc acctggcatc ccagcgcttc gagtgctgct ttggtctgct
    20221 tgatttggat gagcatgccg ccgtgcgcca gcagcgccgg ttccgggtga tcaaacagaa
    20281 tggtcatgcg ttgattgcca gggctttatt gccaccacgc cggacgggag ccttcgttgc
    20341 cgtccccggc cgccgctggc actatcatgc cgacggacgg ttcgaggtca agccatcaac
    20401 cgcgccagtg gtcttggccc gcccgggaac agccggggtt aaagctgctc gatttcaggc
    20461 cagagcggtt gcaaatccgg gtcgctgagc gccaggcgtt tgatttgctc tttgccgccg
    20521 atgcccgccg cccgccgcag ccagaggcgg gcgacgtcca gttgttgcaa ctggcaggcg
    20581 taacaggaga gattgaaggg gatgatggct tcctggggga acttgaccga tgccgggagc
    20641 agcgcttccc aggccttttg caggccgccg tttggcaccc gccgcagggc gtaagcctgg
    20701 tgcagccaac ccgacgagcg ctccgggtcg cttcggagca gagcctgggc aacctgcaat
    20761 ccctcttccc attgctgctg cgccgcaaag agggcccatc gcaactccag cacatcggga
    20821 tgctcctgcc gcgccgggct gacctggtcc agttcggctt tggcttccac gggattgccc
    20881 aactccagcc acccttcggc agcccgcaga taatgattgt caggcggttc cagtttctgc
    20941 atcactgcga atacaattaa ccgttcttag cgcctgtcaa gggccgccac ggtaaagagg
    21001 aggcaaaagc agggacggaa ccctcttgcc aatcgtgccc tcaaaaattc attgtccccg
    21061 cccctgactt ccggcaggct tccgggctgg taattctatg caccaggtaa atttgggaat
    21121 gatcggtggc ggcactgtgg gtagcggtgt gtttcacgcg ctccaacgga atggcgacct
    21181 tctggccgcg cgcattggcg tgcgggtcac ggttcagaaa gtcgccgtta aagccgttga
    21241 cgagccgcgc ccgtaccgga ttccgcgggc cgtcatgtgc acggattggc aaagggtagt
    21301 gcaggatcca caggtgaacc tggtggcaga actggccggg ggcacgacgg ttgcccgcac
    21361 gatgatcctc acggccttga aactcgggaa gccggtggtg accgccaaca aggctctgtt
    21421 gtcggcgcat ggggaggaat tgtttgcggc ggcgcggcaa tatggcacga acctttacta
    21481 cgaggcgagc gtttgcggcg gcattcccat catcaaggcg ctgcgcgagg ggttcgtcgg
    21541 caaccgcatc acccatcttt acggcattgt caacggcacc tgcaactaca tcctgacgcg
    21601 catgaagtgc gagggggcgg agtttgccga cgtgctcaaa gacgcgcagg cgcaggggta
    21661 tgccgaagcc gagccgtcgc tggatgtgga tgggttcgac gcccagcata agattggcat
    21721 cctggcatct ctggcgcatg ggttttgggt gaaccctcga gatatttacg tggaaggcat
    21781 ccgggccctg acgcgcgcgg acattcaatt cgccggccag cttggctata ccatcaaact
    21841 gctggggatt atcaaacgga tcgaggaaag cccggcccga acaccgcggg ggcgcatccc
    21901 gtccgcccgc attcaggtgt cggtgtatcc ggcgctggta ccgaacgcgc acgtgctggc
    21961 gagtgttaac cacgttttca acgccgcatt tgtccgcggc gatattgttg gtgatacctt
    22021 gttttacggc cgcggcgcgg gcaaagatgc caccgccagc gcggtgttga gtgatttagc
    22081 ggacgcggcg ctggatttga agtttggcac tccgcagcgg gtgccgccgt ttgtaccgca
    22141 cgaacgtgcc ggcgcggtgg ttccgatcgc agaagtggtg acgcgctatt ttgtccggct
    22201 gaatgtggtg gaccgcccgg gggtgctggc gcaaatctcc gcgcttttgg gggcggcccg
    22261 gattggcatt tcctccgtaa tccagcccga gagccgcgag ggggccagcg tgccgctgat
    22321 cctgatgctg cacgacgcgc cgagcggcgc cgtgacgggc gccttgaaga agattggccg
    22381 gttggcggcg gtccggagcg cgccggtaat gatccgggtg gaagacttat gctgatcgtt
    22441 caaaagtatg gggggtcgtc ggtagccaat accgatcgga ttaagcacgt tgcatcccgg
    22501 gtggcccaat gccgggcccg gggcgaccgg gtcgtggtcg tcgtttccgc catgagcggg
    22561 gtgacggaca atttaatcaa gctggcccgg gaaatcatgc ccctgcccaa cgagcgtgag
    22621 atggacatgc tgctggccac gggcgagcaa accaccatcg ccttgacggc cattgcgctg
    22681 cactcactgg gcatccccgc cgtatcgctc acgggtgcgc aagccggcat cgtcaccgat
    22741 ggcgtgcata ccaaggcccg catccaaaat atcaccccgc ggaagattca cgaactgctc
    22801 aatcagggcc aggtggtcat tgtggccggc ttccagggcc agacccgcga aggccaggtg
    22861 acgaccctgg ggcgcggcgg ttcggacctg acggcgattg cgctggcggc ggcgatcaaa
    22921 gcggatttat gccagatttg caccgatgtg gacggcgtct atactgccga tccgcgcctc
    22981 gtgccggacg cgaaaaagct cgaggaaatc tcctatgatg agatgctcga actggccagc
    23041 cttggcgcga aggtcatgca gtcgcgttcg gtcgagtttg ccaagaagtt tggcgtcgtc
    23101 tttgaagtcc gctccagcct taacgatcac ccaggtacca ttgtgaaaga ggaaaccaaa
    23161 aatatggaag ccgtcgtcgt gcgcggcgtt tcgctcgaca agaaccaggc caaagtcacg
    23221 ctggtcggcg tgccagacaa gcccggcgtc gcggcgcgca tcttcaaagc gatcggcgac
    23281 gcgggcgtaa acgtggatat gattgtgcaa aacatcagcc acggcagcgg cgcgcccgcc
    23341 acggaccttt cgttcaccgt ggacaaacct gacttgcccc gggcgcaaaa gaccctcgac
    23401 gccctcaagc cggacatcgg ctttggccgg attctggcca cggaaaagat cggcaagttg
    23461 tccatcgtcg gcgtgggcat gaagagccat accggcgtcg ccggccggat gtttgacacc
    23521 ctcgcccgcg agggagtcaa cattgacatg atctccacca gcgagatcaa aatttcggtg
    23581 gtggtggacc tgggcaaagc cgagcaggcg atgcgggcgg tgcacgccgc gttcctgggt
    23641 taatccgcgg gcgggtcgcg gcccgaacca aggttgacaa gccggggcgc gctgcgtagt
    23701 ttggcgccca taggtgcggt tatgagtaca gcgctcaatc aaccggtgtt ggtgctgaat
    23761 cgcttgtggc aggcggtgaa tgtttgctcc gcgcgtcgag ccctgacgct gctgtttcag
    23821 ggccatgccc aggcggtgct cagccgctgc gatggatctt tccagacgtt caacttcaac
    23881 cagtggcacg acttttcgca gcaagagccc ggagcggacg cggtgcgcac cgtctcattc
    23941 cgcatccgcg tgccgcgcgt aattctgctg ctgatctacg accgcctgcc ccgcaaggaa
    24001 gtcaagttca cgcggcataa cattttcgag cgggaccaga acacctgcca gtattgcggc
    24061 caggtctgcg accgcaagga cctcaacctg gaccacgtca ttccgcggga ccggggcggg
    24121 ccgacaacct gggaaaacat tgtgtgctcc tgcatcgagt gcaatacccg caaagccaat
    24181 cgcgcgccgc atgaagccgg catgcgcctc attcgcaaac ccaagcggcc caaatggcgc
    24241 ccgttcgtgc aggtcaattt caccctgcac ccgcacgaca gttggaagca cttcgtggac
    24301 ctggcgtatt ggaacgtcga gttgggcgaa gacgtgcgct aggcgccgcg cctccggttg
    24361 cggccattca cgtgctggcg gtcttggccg cttcaatcag ttcccagaac ttgggattcc
    24421 gctgcagggc ctcgtcctcc ggcaccttca ggcccgagcg aaagagaaaa tccttgagcg
    24481 tgtgccggtt caaaatgcgg tgcacgacca ggcccagctt atggcgctcg aaataaacgc
    24541 ggtaatcacc gacgcggaac cggtgcagca tccggcccgc gcgctcgagc ttgccgaact
    24601 gggctaattc cgtccccagc acctgctggg gcaagccgcg aaattcaccc aggatcttga
    24661 gttgcagctc tttcggcatc cgtgccagct cggcggcgct ggtgggattg aatatgattt
    24721 gaaaaaggcg catactctaa gagaaggcag aattgcccgt gggtcaagcc aacgctttcg
    24781 ctgtcgcggt tgcgggcgct ggccgggagg acgggcgatg agcgctcccg agggcggcgc
    24841 cgccgccgcc ggcgttcgtt tcgcccgcgc cagcggccct tcggctttgc ccgccgccgc
    24901 gattcatttc aagtcgcgca cggcctcttg gttgccctcc tcccaaccct ccgagtgggg
    24961 gttcctttcc tgagccagcc gatttgcaat aaaaccgccg gggccggata accgggcggc
    25021 ccggaggcga agacgcgccg ggccgcaaat tcttttacat tgggttgcca ggtgattaaa
    25081 ctgagcggca gtgaaaacat tcatcaaaac actattgata ctggcactgg ggcttccggg
    25141 gatggcgccc gcggaagtca agctgggcaa cgaggtgctg gtcgaagggc aattcaaggt
    25201 gctgcaaggc aagcggcttg gactgattac caatccttcg ggagtgaatc ggaatctcga
    25261 gtccaccgtc gaggtgctgc atgccgcccc gggagtgaag ctggccgcac tgtttggccc
    25321 ggagcatggg gtttatggcg acattgaagc gggggaaaag gtggatacgc tggtggataa
    25381 gcgcacggga ttgccggcgt attcgctcta cggcaaaacg cacaaaccga cgcccgaaat
    25441 gctgaaggat ctggacgcgc tggtgtatga tttgcaggac acgggctgcc gctcttatac
    25501 ctacatcagc accatgggca tggcgatgga ggcctgtggc gaggcgggga tcgagttcat
    25561 cgtgctggat cgccccaacc cgctgggcgg cgaacgcatc gagggcgcga tgctggagga
    25621 gaaattccgt tcgggcgtca gcaaatggaa cgtgccctac gtttatgggc tgacctgcgg
    25681 ggagttggcc cgactgctca acggcgagcg gctcatcaag acgccgtgcc ggttgacggt
    25741 cgtgccgatg aaaggatgga agcgctcgat ggtatggtcg gacacgggct tgccctgggt
    25801 gccgagttct ccacacgtgc cgcatgggca gtcggcgctc taccaggtgg ccaccgggat
    25861 gctgggggaa ctgatgagcg tcagcatcgg aatcggctac acgctgccgt ttgagtgtgt
    25921 ggcatcgccc gggctggatt cgcataagct ggcggaagcc ttgaacgcgt atcggttgcc
    25981 gggggtaaaa tggcgcccca ttacctacaa gccatattat ttcacctttt ccggccagcc
    26041 cctcgccggc gcgcagctct attttaccga cccggcccgc gcgccgctga ccgcgatcaa
    26101 cctgtacgcc attgaggccc tgaaacgggt cggcggagta gatgcgtttg ccgaggcgga
    26161 aagagtgaaa cgaggattcg gcatgttcga caaggtcaat ggaaccgatg cgacgcgcaa
    26221 ggccctgcag gcggggactc ctgccgctga tatcgttgcg tcctggaaag ccggcgaaga
    26281 agctttccgc aaagtccgca ggaagtacct gctgtactga ggtccgcccg cgttctctcg
    26341 cgagttgact caatccggcc aggaggtttt tatagccttc atgcgagtct tacttttggt
    26401 gatcctcccg ggcgtgctgg cgctctcagg ttgcgttggc cgaagggaaa aagctccgct
    26461 gcctccggac gcgccccgcg cggaaacccc ggccaccccc gccgagcccg ccgcgccaaa
    26521 tcctcccctg attgtaaccc cggacgccgc gccggtgggc aaggtggcgt tggtcaacgt
    26581 gcccgcccgg cacgtggtgc tgacatttcc cttgcagaag ctgccacccg tgggccagcg
    26641 gctcgcgctc taccggcacg gcgtggcagt cggccaggtc aaagtctcag gcccgcagcg
    26701 ggaggacaac atcgtggccg acctaatcgc cggcgaagcg gaggttggcg acgaggttcg
    26761 gagtccgtga ccgcggccgc gcggaacagc cgccgacggg ccaggcttgg aaataccccg
    26821 gctcagccgg gcagaaattt caccacaaag tggtttccac gtactgatcc ttggccttga
    26881 tgcaattcgt gaaggtgact ttggttccgg aaggaacgcg gtcccgcagg gtctgaaacg
    26941 gataaagcgg caccgcatac atcagtttgc cgtattggat ctgaaagttg atatcactca
    27001 gattggtaat ttcgatcatt ccccgttcct tgttaatgac cttgatcgaa agcgcggcgc
    27061 ggatgagcgc cttgagatat tcctccttgc cggagagaat atggttataa caggccaccg
    27121 ttcggccggc taaaagggct tccttgaccg acgcttcggt gtattcctgg gcaaagacca
    27181 gggtcatggg gcgcaggcca cgtccccggt agagttgggt gccaatgcca tgaatatccg
    27241 tgttggcaaa gggggccagt ttcaactccc agcaccagtc aatcactttg ggataagcct
    27301 caaacgagtt gaaaacctcg actccgtgga tttttccttt ggcaatcaac tcccggtgca
    27361 ccgggtagat ggtgctgtcg tcatccggcc agccgggatg gttccacaaa ataaaagccc
    27421 cctgcgccag ggcttcgtca atcgcgtcga gcgggtcagg gacctccagt ttattggcat
    27481 ccttaatgaa cagcgcatta agatgcccca aaggtttgct gcgggtgatc tcggagccgg
    27541 aaatgacgat catccccagt tcctcgccct tcttccgggc gatctcgttg gacttgttga
    27601 aatcaacgtt caacagcgcc ttgtggggcc gatattcaat atggtcggta atggcaatga
    27661 catccaggcc ctggttccag gcttccaaca cccgctgatc cggccaaagc tttccgtccg
    27721 aaaatgccgt gtggatatgg aaatcacact tcagggtctt atatccttca atatcgggca
    27781 cgaccaggtg ctgccggtaa ttctcctcgc gaaggttcgg gtattcataa acttcgtcat
    27841 ttctgatttg ggcccgcgca gttcctccga cgcacagtat tcccagggtg acggctaaga
    27901 gggcttttgc tttcatcatg ttctttgcta accagggggt gttggtgaag cgcgtccggc
    27961 actcagcatt gcgcggatca ggcttcgggt tccgggggat cgccctccac cgattccttg
    28021 aggggcttca tggttttggg cagcgactcc ctctggtagg gcgagtggag attgaaataa
    28081 atcccgcaca acggccgggg gctgtcgctt tggctgagaa tgaccgtgac ctgtttgtcg
    28141 agagcaatgc catggcgcaa ttccgggtcg cgtccctcgt aggcccggat ggccttgccc
    28201 agcaaggact tcagcgtggc cttgaattca tccggggtct tattaaggca cacgacgtat
    28261 ttctcgtaag ccttcacggc ttccgcaatc tcgcggcgaa actcttcagt ggtcacgatt
    28321 ccagcctacg cggaaccgcc ctcgaagtga agtccaaaag caaaccagct cacgccgtcc
    28381 gcaggtccgt ttgttatcgg cgtctgagtt tcggtagctg agggggaaaa gatttttcca
    28441 aacccaaagc cgctttccga gctccgggag ctggggtaaa agcgtctctt gcttcaaagc
    28501 agccggccaa ccctgcgggc gtggttggcc ggcgcgaggt tgccgaaatc gcccgctttg
    28561 ccatcgtctt gacgcaacgc caacaccacc gtttgggatt gccacagaag aaggggcccc
    28621 caagcttcca tgaggggccg ggctatgccg tgttttgcca gatgctcacg cgcaggaacc
    28681 ccaacgcctt tgccgcgcga taagacaccc cctctgaaac gctgaaccgt catccaaccc
    28741 gcagccacca cctccccggc tcaagatcta cacaaccaac gcccctgaac aggttcccga
    28801 accaatcccg tcaatcattc accttgcgca catagcctgg agataaatag ttgtgtccgt
    28861 caagttcagc aaattggtta tcatggtttc cactcaaggg gtt
//
LOCUS       DAOPQG010000040        28458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2135_length_28458_cov_15.271661, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000040 DAOPQG010000000
VERSION     DAOPQG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 28458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28458
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_28458_cov_15.271661"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..466)
                     /locus_tag="PKI07_06365"
     CDS             complement(47..466)
                     /locus_tag="PKI07_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50714.1"
                     /translation="MTSFDPASVRQAVAEFTPRRPEKFQDLMPAKEVIVELRQKRASY
                     RAIAELLTQHCLPTSKTAVAVFCHEVLGEIVRPRRRPGRKRPIVPAVADAASASAPSL
                     ATQTPTEPTGNGSGEDSPSRSRGPRIAQVRMLKPQTS"
     gene            complement(922..1176)
                     /locus_tag="PKI07_06370"
     CDS             complement(922..1176)
                     /locus_tag="PKI07_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50715.1"
                     /translation="MQTPTTTQLHTAIEVLKKLGERLNTHAENSAMQLAESPVTAHQA
                     AQTGVGAIEQTTRIEGVVTQLEQWRTELNQQRRQCVTHHV"
     gene            complement(1371..1649)
                     /locus_tag="PKI07_06375"
     CDS             complement(1371..1649)
                     /locus_tag="PKI07_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50716.1"
                     /translation="MITLNMQHLSVSTPALRGKPDTLFGDVVNYSDHDLEIVADNQGT
                     GRAPCVTDTSAHLPIVLNCWGEHENDSKARHQWLAHLPHQPEIRSRRR"
     gene            1642..2307
                     /locus_tag="PKI07_06380"
     CDS             1642..2307
                     /locus_tag="PKI07_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOC50717.1"
                     /translation="MIKGWFGEKLTTFGMWLRLDETVYRRIDDLIIPSKNGTTQIDHV
                     LVSVFGIFVIETKNMQGWIFGGEKDTTWCQSVYGHKNRFQNPLRQNYRHIECLADFLK
                     LDRHHFHSVVFFVGECEFKTPMPANVLCTGLGSYITSINSPVLTQQQVMDIEGRLREF
                     KATGGLTKSDHLESLWQRHESTDVCPKCGGKMVARTAKSSGKQFLGCANFPKCRHTIW
                     PNC"
     gene            2359..5211
                     /gene="mobF"
                     /locus_tag="PKI07_06385"
     CDS             2359..5211
                     /gene="mobF"
                     /locus_tag="PKI07_06385"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HOC50718.1"
                     /translation="MVTAKTQYNLANAREYFEEHLCIGDYYDEGQRVSGEWQGLGAER
                     LGLVGRVRAEDFLRLCENQHPATGETLTQRLNTTRAVGDGSAANRRIFYDFTFSPPKS
                     VSLAGFLGEDTRVLDAHARAVKSALHEFEAFASTRIRTGGAQKDRRTGNFAAALFTHD
                     TSRALDPHLHTHCIVFNATFDDSENRWKALQNYELLRARKFAENAYYHELARELRGFG
                     YRIRNLPRGDFHIEGIPAELCDRFSKRDAEIDKALAKLLADKPELAGSNIADLRARLA
                     TEKRTRKQKDLSRDELRSLWGAQLNQADREVLRGLTTSPSDDAKRRASISVVEAVQWA
                     EEHLFDRNSVVLECQVWQEALGRARGEDFSLAELKQLTERRGYIRDAARPGELTKRDV
                     LLREWEIVQTAKEGVGDCWPLVLNPQPANPKLDDEQRKALDGLLGSTNLVSIFRGGAG
                     TGKSFVLHELVQQIKQSGRPAAVLAPQRQQVVDMEKAGFPSPATVASFLTKAELPERA
                     VIVVDEAGQIGGQQMLALLRLAKERNARIVLSGDTRQHGAVEASDALLAIERHAGIKP
                     VELHKIRRQGPGLARDNSERASIKRYRSAVEAAAAGRLADSFKRLDQMGAVVGCGLGE
                     QADKLAEEYVCLAEKSASAVVVSQTWGEVHRVNARVREALKAKGLLGASDTPIQALER
                     VDLTNAQKRDERFYPPDAVIVFNQKVREAEVGSKGKLSGILKTGLLVEVGGRFVTVSN
                     KMLDRISVCQAREIAVASGDRLHLKANRRLSSGGRVTNGELVAVKSVRGDGSIELADG
                     RVLDANFREFLAGYAVTSYGSQGKTVDYVLFSDSSIKAATHAQQWYVTISRGRRGIRI
                     FTPDKEQLRENVTRSGHRPLALELATGLTPRRGVRLWDRLHGYMLRFGRRAADNFCRL
                     KISRHRHQPIETHEHKNTRMLGERPERSRGQNRSIR"
     gene            5138..5485
                     /locus_tag="PKI07_06390"
     CDS             5138..5485
                     /locus_tag="PKI07_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50719.1"
                     /translation="MSTKTPECWASDPNARAVKIEVSAEQSLLLPFDQFAFAELKNEG
                     KEQRLRLVFATHEVTLRGHTLRRIETAIQRMELAFLGALPAHQRALVTEGQPLITAID
                     VSVAAEKEKPSPD"
     gene            complement(5482..5847)
                     /locus_tag="PKI07_06395"
     CDS             complement(5482..5847)
                     /locus_tag="PKI07_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOC50720.1"
                     /translation="MASNYDDHLRNHGFLMFHPGRWSLSPAYDLNPVPEVDRVHMGKT
                     AITEEQEEPTIAVALAAAERFGLKPAEAKRILREVFTAVAGWRATGRKLRLTAGVLDT
                     YASAFDHTLMTEAAQVLGV"
     gene            complement(5802..6710)
                     /locus_tag="PKI07_06400"
     CDS             complement(5802..6710)
                     /locus_tag="PKI07_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOC50721.1"
                     /translation="MSLEVHVDWQGESHLVGRLDVAERSASVSFEYATEWLQRPDAFA
                     IDPTSLPLQRGVQHGKSLFGAIQDCGPDRWGRVLIERAVRKKVMAQKPYRDIDYVLAL
                     DDAARIGALRFRINTEGPFLAPTSGKLPPLVKLAALLRATDAIHGETETAQDLRFLLG
                     AGSPLGGARPKAAVSLPDDRLAIAKFPKPDDTRDIGAGEILALTLAAQAGIRVAEHQR
                     VAVGMQSVAVITRFDRNGHQRVTFISAASLLGLPQGEAGAYTMLADGIRQFGDDVPGD
                     LRELCGDWSFHFWPATTTTICAIMAF"
     gene            complement(6707..6916)
                     /locus_tag="PKI07_06405"
     CDS             complement(6707..6916)
                     /locus_tag="PKI07_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOC50722.1"
                     /translation="MAARLFVGRNTLWRLERGDPTVALGTLVTAAFILNLHDRLANLA
                     APASDAVALSLDEQRLPKRIRRKKS"
     gene            complement(7167..7712)
                     /locus_tag="PKI07_06410"
     CDS             complement(7167..7712)
                     /locus_tag="PKI07_06410"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOC50723.1"
                     /translation="MNTTENLITTGCPTTALQPQNKIVKPIKFPSQPQEWKIVSLREC
                     PTPDTMQQCETPDQAAAYWKTHIATHPYFNSECECLVVFILNTRRRIKGHYLVSIGTM
                     DTILCHPREVFRLAIMASAAAIIIAHNHPSGEATPSEADIKITRDLIRAGQLLKIEVL
                     DHIVIGNPSHQSLRSLGYFYS"
     gene            8716..10011
                     /gene="hisS"
                     /locus_tag="PKI07_06415"
     CDS             8716..10011
                     /gene="hisS"
                     /locus_tag="PKI07_06415"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOC50724.1"
                     /translation="MERLPGFRDFYPEPLPQPGLWSANARQYIFNTWRSLARRYGFHE
                     YDGPPLEPLELFTTKSGEEIVGQLYHFTDKSERQVSLRPEMTPTLARMIAACERGYKK
                     PFKWFALPQLFRYERQQKGRLREHFQFNADIVGEKDPAADAELIALLIDTLRSFGLTH
                     EDFVIRLSSRNAWQVFFRHRHGAATQEYAFYQIIDKLEREAPEQSNEKLERLGFSLAE
                     VSSFIAQGEPTAELTAILANLAARGLRDYVKVDYHVIRGLAYYTGVVFEAFDRKGEFR
                     AIAGGGRYDNLVKLISQGRVDLPALGFGMGDVVLLELLRARALLPKFTAALDVFCLVE
                     DENLRVEFLKVIHDLRAAGLAVDYSLTAAKPDRQFKRAQELNATYTLKLERDPAGMLL
                     ARIKNLEARQESVVLPAEVVTWLKGRQGQEGFRGDPAKA"
     gene            complement(10082..10471)
                     /gene="rplS"
                     /locus_tag="PKI07_06420"
     CDS             complement(10082..10471)
                     /gene="rplS"
                     /locus_tag="PKI07_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOC50725.1"
                     /translation="MNQALLDKIESAQYRKDALDFGVGDTVRVHTKVVESGKERIQVF
                     AGVVIGRRGHGLNEMFTVRRISYGEGVERVFPLHSPRVAKVEVERKGATRRAKLTYLR
                     KRVGKGATLVKEKETRTAATASRGVKS"
     gene            complement(10452..11147)
                     /gene="trmD"
                     /locus_tag="PKI07_06425"
     CDS             complement(10452..11147)
                     /gene="trmD"
                     /locus_tag="PKI07_06425"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOC50726.1"
                     /translation="MKIDVLTLFPGMFVGPLDESIIRRAREAGRLELALHNLRDYALD
                     RHKTVDDRPFGGGPGMLLKPEPLFAAVEDLACENTCVLLVSPAGRRFDQSIARELASR
                     EHLLMVCGHYEGFDERVRERLADDELSIGDYVLTNGALPVMVIIDAVTRLLPGVLGDE
                     DSAREDSFSQELLEYPQYTRPAEFRGMKVPEVLLSGNHAEIARWRAEQARRRTQERRP
                     DLFAKTFYESGIT"
     gene            complement(11232..11477)
                     /locus_tag="PKI07_06430"
     CDS             complement(11232..11477)
                     /locus_tag="PKI07_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOC50727.1"
                     /translation="MQAFLEYVVKGLVQNPEAVTITPVEREGTTVYELRLHPQDVGKI
                     IGRQGMTINAIRSLLLAGSARKGQRCTLEIVEDRPAT"
     gene            complement(11501..11761)
                     /gene="rpsP"
                     /locus_tag="PKI07_06435"
     CDS             complement(11501..11761)
                     /gene="rpsP"
                     /locus_tag="PKI07_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOC50728.1"
                     /translation="MAVKIRLKRIGTKNTPAYRIVVADSRSPRDGRFIEELGTYLPQK
                     KSDKFTLKLDRAQYWVSKGAQPSETVASFIKKATKAAAAATA"
     gene            12111..13790
                     /locus_tag="PKI07_06440"
     CDS             12111..13790
                     /locus_tag="PKI07_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50729.1"
                     /translation="MKKIVLACIIVAVGCLGLWFWARPAYRRHQEIRLVEQAKAYLTQ
                     KEYNNASLSARRALQINPRNLEACRVMADLAEKTHSPDALDWWRRIVEAEPSIPHKLQ
                     FASAALHSQSPPFPLATQILEELKGTATNLAAYHAISAELALRLKRTTVAAKHLEQAC
                     RLEPTNELHQLNLAVLQLESTKAGVPSAARVTLERLRASTNVGDVALRWLVAESLERN
                     DFSRAARFSRQLLADPRVVMGDRLQHLAILRQSQSPGFKDYLRTQQRNATTNAAQVYA
                     LSTWMVERGLADDALTWLLACPAKLQAEQPVLLAVADCYMARKDWHGLDQALSAQNWG
                     DREFLRFAFLARAVTELNQKLAADAHWRTAIRNAGDRLGSLTTLLTLATKWGQEQARE
                     DLLWRIAQRFPRDQWALRELERTYTLAGNTLGLNKVYSSMANYAPQNFVAQNNLAATS
                     LLLKLNLPRTHELARELFTQHPEQAVIASTYAYSLYLQNRTREGLAVLQKLKPEDLEN
                     PSVALYYGILLTAVGEGNKASPYLRIAQDSSLLPEEKILLAEALKRPAPNS"
     gene            complement(13821..16940)
                     /locus_tag="PKI07_06445"
     CDS             complement(13821..16940)
                     /locus_tag="PKI07_06445"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOC50730.1"
                     /translation="MIVANEQGLSKARPAGRRRAAAVTGARTPKKRAKAAAGKTLKIE
                     RWFSDPKVSPFDQVEWERRTAEITDDGGKVIFRQENVEVPKAWSLLASTVVVSKYFYG
                     EQGTPERETSVRQLIHRVCRTIADWGVKDGYFTRAEGEVFYDEMTWLCLNQYGAFNSP
                     VWFNVGLYHQYHIGNSSSRGNWVWNPKTGQAERAATQYESPQCSACFIQSVADNMESI
                     MELAHSEAMLFKFGSGTGTDLSPIRSCKEKLSGGGRPSGPLSFLKVYDQVANVVKSGG
                     KTRRAAKMNTLRDWHGDIQEFIEAKMKEERKAWALIEQGYDGSYNGDAYGSVMYQNEN
                     LSVRVSDAFMEAAEAGREWWTRSVTTGKPLEKKDARLLLQKIAEGTWVCGDPGLQYDG
                     AIQKWHTCKGTEPIHSTNPCSEYVFINNTACNLASLNLVKFKRADGIFDVAAFKAAVR
                     IFITAQEILVDNASYPTRQIAENSHIFRTLGLGYANLGSLIMSYGLPYDSDEGRALAG
                     ALTAIMTGRAYEQSAEIAAIMGAFKGYHDARCAHVTAPLAADNVDSMLAVIQQHRDAV
                     EKIQPSREFDYLKEEARQSWTQALARGRATGYRNAQVTVLAPTGTIAFSMDCDTTGIE
                     PELALVKFKLLAGGGSLNIVNRTVPEALQRLGYNAAESERIIGHITQYHTIEDVVEGA
                     QTITSGLRPEHLPVFDCAFKPSRGQRSLPYLAHLKMMAAAQPFISGAISKTVNMPNES
                     TIEDIRDAYVQAWKMGLKCVAIYRDGSKRSQPLSTKRLGEGEGKAETGSIFALEDRIK
                     ELEAEITQLRTQSGKPLRRRLADTRTAITHKFDIAGHEGYLTVGMFESGQPGELFITM
                     AKEGSTIGGLMDGIGTLTSLALQYGVPLETLARKFAHVRFEPSGFTKNRDIRNAASIM
                     DYVFRWMAIQFIPGFREAHTSNQNQPELAIPGLHEAEKKKVNRPVRELPLADDTEILE
                     LKSGADGNGKGHSPAQPAPAMRPAKTINELVAHFQQDAPACPNCGNVTVRSGACYRCL
                     KCGESLGCS"
     gene            complement(17239..18765)
                     /gene="der"
                     /locus_tag="PKI07_06450"
     CDS             complement(17239..18765)
                     /gene="der"
                     /locus_tag="PKI07_06450"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOC50731.1"
                     /translation="MSRLIAIVGRPNVGKSALFNRIAGRRIAIVHDQPGVTRDRVAAE
                     VEWQGHPFTLVDTGGIGLLRREKAEDVIVKAALEQVELAIEAADVIILVVNVQEGLLP
                     LDREAAARLRRSGKPVLVAVNKADTDRAESGVPEFAALGFPQMFPVSAIHGKGIEPLM
                     QAAMSRRQSGASPAAPGVAAESPGEVPGTAAFSAPGGARPAALKLAIVGRPNVGKSSI
                     INALTRSERVVVSPIPGTTRDAVDVPFEVETEGVRQSYVLIDTAGLRKTRRVGDSIEF
                     FSVKRAEESIARCDIVILVLDAEAGITEQDKKIADRIVENHRACIVVINKWDLMDDAV
                     RAARRQEIRRRQNPKGRTAPNVMTTLGEFGQWVQERLFFLDYAPVIFTSAKSGFHLDR
                     LLEAVRYVAAQLQQSIPTAILNRTLRDAVEQRQPVSAQGYRLKFFYATQVRQAPPTFL
                     LFVNRDELFSEPYKKYLANQMRQAFGYEGCPIIIVAKPRPKTIAPVRKFRGAGRQKAG
                     "
     gene            18886..19353
                     /locus_tag="PKI07_06455"
     CDS             18886..19353
                     /locus_tag="PKI07_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOC50732.1"
                     /translation="MLYFAYGSNMDPAQFVRRCPSACFVTVAQLPNHQLAFTRLARDR
                     GCGTCDGIPAPGKNIWGVVFAISKADLQRLDESEGYRPGRPLADNAYVRKQRQVCRPG
                     DPGKRLRVWLYFANRQPHPPLPNAAYKQQLVEGARLWGLPPAYREELARIQTA"
     gene            19625..19756
                     /locus_tag="PKI07_06460"
     CDS             19625..19756
                     /locus_tag="PKI07_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50733.1"
                     /translation="MGRMRYPVMGTGQPMPASIPNAAGAAGISRPTGLVTNLLRFGP"
     gene            19818..21218
                     /locus_tag="PKI07_06465"
     CDS             19818..21218
                     /locus_tag="PKI07_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015849001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC50734.1"
                     /translation="MTEKELKRSVLTVTAFASFLTPFMGSAVNLALPSIGEEFSLNAV
                     MLSWIINSYMLAISMLLLPAGRAGDILGRRKVFTAGLVIFTLTMLLLAITPDIKWLIG
                     VRILQGIGGAMMFGTSMAILTSVFPPGERGRAMGINVTAVYMGLASGPFLGGMFTRYL
                     GWRSIFAFLIPLGIASLLLIRSKMKLEWAEAKGESFDWPGSVLYGLSMAALVYGFSKL
                     PAAAGWVLTGAGLVLMPLFVIREKRAVHPLFDLTLISRNRVFALSSLAALIHYAATSA
                     VGFFLSLFLQYIKGLGAREAGFLLMSMPLTMALMSPAAGKLSDHHNPGVLASIGMAIT
                     ALGLILLCFLVEQTSVTFIAVILVVMGAGFSLFSSPNSNAIMSSVEKRQLGNASGMLG
                     TMRNVGQTLSMAIALMLLAIYMGQEKIHPGNYPQLMRSMRTGFIVFSILCFAGIFASL
                     ARNQGLANNRGNRRSF"
     gene            complement(21273..22316)
                     /locus_tag="PKI07_06470"
     CDS             complement(21273..22316)
                     /locus_tag="PKI07_06470"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC50735.1"
                     /translation="MTLSAPAASDRVARLTPQLDALFQNFNQPESPGASVMVIHRGKV
                     VVAKGYGLANVEEKIPCGPETNYRLASVTKQFTAMAVLILADRRKLSFEECLTDFFPE
                     FPAYGRQITLRQLLNHTSGLLDYEDLIPPGTELPVLDRDVLRLLMRQDKTYFPPGTKY
                     RYSNSAYALLAEIVTARSGLTFPRFLQQNIFRPLKMKGTLAYEGGVSVVANRAYGYSA
                     AAGGFKRTDQSLTSSVLGDGGIYSSVADLYRWDQALYTDKLVSRKLLQQAFAPGPATD
                     LPDTRYGFGWYISQYRGAREIWHRGSTIGFSTRLVRFPEQQFTVIILTNRDEAGITEF
                     AHRLADWYLFDRP"
     gene            22987..24279
                     /locus_tag="PKI07_06475"
     CDS             22987..24279
                     /locus_tag="PKI07_06475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC50736.1"
                     /translation="MKLLIFAHTPPPHHGQSYMVQLLLEGLGGDHRRRKRRAAGSGPE
                     TSFGIECYHVNARLSQKLEDIGDLRVSKLILLLGYCLQAIWCRFRYGVTNLYYIPAPG
                     KRSALYRDWLVMLLCRPFFKRVILHWHAAGLARWLELAVSMRWRVLTYRLMKQVDLSI
                     VLSQYNRADAEKLFSRQIRVVSNGIADPCPAFAGEILPRRKARFAARQKLLSRQPLEA
                     DEARLAGAEPHLIQVLYLAHCTPEKGLFDTLSAVALANERLQQRQSPVAFRLVVAGGF
                     VTNEARVKFDQRIARPDLAACVKYLGFVSGAQKNQWLREADLFCFPTYYENENQPVNL
                     IEAMAFGLPILTTRWRSLPELFPANYPGLVDIRAPEQAADRLLALITSETGEELRDIF
                     LRHFTLTRHLTAMAEALRQAETPPPAAVPAAAVRSLPG"
     gene            complement(24299..25258)
                     /locus_tag="PKI07_06480"
     CDS             complement(24299..25258)
                     /locus_tag="PKI07_06480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC50737.1"
                     /translation="MELWGLGDLVIATPFLQAASRSYAVTLVAKPYAVDLQKRFWPEV
                     RVEPFVAPWTAFKHKYRLLRWPWREMLRLQRRLVAKRFDFGLSARWDPRDHVLLLLAQ
                     VRRRLGFARWGSQMILSATPARPDPTEHRYEHWRVMARQLGIEVPPRAALAWPRSNPE
                     GGILVHTGAGQPVRVWPLERYHTLVARLRRNHHSVQIACDPDQREWWVKAGETNVAAP
                     RTVTELFALVDRAGAFIGNDSGPGHLAAFCGVPTFTLFGPQMAEWFAPLNPASEWLEG
                     KACPYKPCSDYCRFPTPHCMFNTQEDEVGARVEAFVARVLRRR"
     gene            complement(25495..26850)
                     /locus_tag="PKI07_06485"
     CDS             complement(25495..26850)
                     /locus_tag="PKI07_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50738.1"
                     /translation="MEQTRPNALAALKANPASPPKPRPRTILIAVVCAALGIGGAVSP
                     AAPARSSAARWQLGAPIITYWAGPGSRTAVNEQAAAQLRAGGWNLAWTQRPEDLDLYH
                     RHGLRVMLEIATPDVEDPAQAQALAALIARVRSHPALYAYYLVDEPGAGAFPKLGRLA
                     AWLRQRDPAHLAYINLLPTYASDGQLQVSDDAAERARVGCPQDFAGLQADDFTALRYR
                     EHLRQFVRIVQPDLLSYDHYHFLKSSDGPQYFLNLALIRAAALETGKPFLNIIQTCDS
                     PAEGWRAPGEDEVRWLTYTSLAYGAQGIAHFRYDTGLWRDPNEQTIPLPLYWAVAQLN
                     RQFLAIATELQPLKSLGAYHCGTIPLGGQALPPESAFATEPATAETLLGYFGKSARRP
                     THVVVVNLNYKNAATLTLNGPGPLEVFHPPTRAWLRPEGGRRIKLNLLAGGGALVRLR
                     K"
     gene            complement(26814..28322)
                     /locus_tag="PKI07_06490"
     CDS             complement(26814..28322)
                     /locus_tag="PKI07_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50739.1"
                     /translation="MTAAIHSSNPAAPHAGATRGQPTKSAMLILAAAVTVNCCAAELR
                     IGTAAISITPDQPVALDGQFNLRVSRGVDNPVTATAVALEARVGDRTTDQAILLSADL
                     VAFRSFIPPRVRERLRAQLPGFDPRKLVLTATHTHTGPVTEEGKYDIPKEGVMPPREY
                     VEFLVARLVDVSVRAWNQRQPGGVSWGLGQAVVGHNRRAVYANGTAQMYGATSTPEFR
                     GIEGGADHGLELLFFWTAEKKLLAAGVNIPCPSQVVESRTTINADFWHDVRERLRAAA
                     GAELGVLGWPGAAGDLSPHWLYRKAAEERMLKLRGLSRTQDIGRRIAGEVLEVSELTR
                     SDIQREVPFAHEVADLVLPARKVTDAEAAEAIKEIEALKGKNDATRRIAWHQATVDRY
                     QAQGDAPTVTAEVHVLRIGDIAIATNPFELFQDYGTQIKSRSPALQTLVIELTGGPSG
                     YLPTPRAVAGGGYSAIVQSGQVGPEGGQELVERTVEIIKRLWSKPAPTPSQP"
BASE COUNT         5409 a         8755 c         8601 g         5693 t
ORIGIN      
        1 cttttgccga cgccaccttt gccattgagg atcaaatcga gtcgtttcat gaggtttggg
       61 gtttgagcat ccgcacctgg gcgatgcgtg ggccgcgact gcgcgatggc gagtcttcac
      121 cgctgccatt gccggtcggt tcagtgggag tttgagtggc gagtgatggc gcggacgcgg
      181 acgcggcatc ggcaactgcg gggacaatgg gacgcttgcg tccgggacgg cggcgcggac
      241 gaacgatttc acccagcacc tcgtgacaga acaccgcgac cgccgttttg ctcgtgggca
      301 ggcaatgctg ggtgagcaac tcggcaatcg cccgatacga cgcgcgcttc tgccggagtt
      361 caacgatgac ctccttggcc ggcatcaggt cttggaactt ctcgggacgg cgcggcgtaa
      421 actccgcgac cgcttgccgc acactggctg gatcaaatga ggtcatgagc gggagcgtgt
      481 tgaatccgga cggggaattc tgtgggcggc gctatggaca cagacagtcg atgaactgcc
      541 agcgtcccat tcgcggacga tttgcgactc gctcgcggct gcggcgtgtc cgtggacgcg
      601 gacattttgt cgtcctggac tgaactggaa cgaatcacgt ccgtgaccgt tgcctgatcg
      661 cgactgttct ggcccgctgc cagttcacgg acacggacaa ttcgttgcca agattgaggg
      721 cgcgggctga atgcgtccgc gacctgttcg cgcttcagat tgcggcgcaa ctgcgcaccg
      781 cccacggcca attcacggac acggactgac tcttgccgtg gccgctggct gccgctcatg
      841 cgcgcttgtt gcgcgtccac ctcgcgactc attcgcggac gcaaaatcct tgtgacaaca
      901 aggggtaagg cttgtccttc cttaaacgtg atgggtaaca cactgcctcc tttgttgatt
      961 gagttccgtg cgccactgct ccaattgcgt gacgacacct tcaatgcggg tggtttgttc
     1021 aatcgcgccc acgccggttt gcgcggcctg atgtgcggtg acgggtgact cagcgagctg
     1081 catggccgag ttctcagcgt gggtgtttag ccgttctcca agcttcttca gcacctcaat
     1141 ggcggtgtgg agttgagtcg tggtgggtgt ttgcatagtt agatgtgaat ccggattgag
     1201 ttttggtgac cgagtgtttg acgatgctgg tggaaagttg ttggcaacgc atctgccagc
     1261 cgaacttggg aacgcgggat cgagtgtatc caggattcgc cgaagccgag atcgccgcag
     1321 cttccttaac accgatggta aatgaatctt ccaccaccct gcgaaacggg tcaccgacga
     1381 cgcgagcgta tttccggttg gtgcggcaga tgcgccaacc attgatgcct ggcttttgaa
     1441 tcgttctcgt gctcacccca gcagttcaac acaatcggaa ggtgggcgga tgtatcagtt
     1501 acacagggtg cgcggccagt cccttgattg tccgccacga tttccagatc atggtcgctg
     1561 taattcacga catcgccaaa cagcgtatcg ggcttgccac gtaaagcagg agtggaaaca
     1621 ctcaaatgct gcatgtttaa tgtgatcaaa ggctggtttg gggaaaagct gaccacgttc
     1681 ggcatgtggc tgcgcttgga tgaaactgtc tatcgtcgca ttgatgactt gatcatcccg
     1741 tctaaaaacg gcacgaccca gatagaccat gtcttggtct cagtgtttgg aatcttcgta
     1801 atcgagacga agaatatgca aggttggatt tttggcggcg aaaaggacac cacttggtgt
     1861 caaagtgttt acggtcacaa aaaccgattc cagaatccgc ttcgtcaaaa ctaccgtcac
     1921 atcgaatgtt tagctgattt cctcaaactg gatcgccacc attttcactc ggtggtattt
     1981 tttgtcggag aatgcgagtt caaaactccc atgccagcaa acgtgctttg cactggtttg
     2041 ggcagttata ttaccagcat caacagccct gtgctcactc aacagcaggt gatggacatt
     2101 gagggtcggc tgcgagaatt caaggcgact ggtgggctca ccaaatcaga ccaccttgaa
     2161 tctttgtggc aacgtcacga atccacagat gtctgcccca aatgcggcgg caaaatggtc
     2221 gcccgcacag ccaagagcag cggcaaacag tttctaggct gcgccaattt cccaaagtgt
     2281 cgccacacaa tctggccaaa ctgttaaagc tgcatgtgat acacaatccg ttgacaggta
     2341 ttgtttatcg ttttgcgcgt ggtcacggcc aagacgcaat acaacctcgc aaacgcgcgg
     2401 gaatactttg aggaacacct ctgcatcggc gattactacg acgagggaca gcgcgtctcc
     2461 ggtgaatggc agggcctcgg ggcggagcgg ctggggttgg ttggtcgcgt tcgggcggaa
     2521 gacttcctgc gactgtgcga aaatcagcat cccgccacag gcgaaacgct gacccaacga
     2581 ctgaatacca cgcgggcagt tggcgacggg agcgccgcaa accgtcggat tttctacgac
     2641 ttcacttttt cgccgccgaa gtcggtttca ctggccggat ttctcggcga ggacacgcgg
     2701 gtgctggatg ctcatgcgcg ggcggtgaag tcggcgctgc acgagttcga ggcgtttgct
     2761 tccactcgga ttcgcactgg tggagcacag aaagaccggc gcactggcaa ttttgccgcc
     2821 gcgctgttca cgcatgacac ttcgcgtgcg ctcgatccgc acctgcatac ccattgcatc
     2881 gttttcaacg cgaccttcga cgattcggaa aatcggtgga aggcgctcca gaactacgaa
     2941 cttctgcgtg cccgcaagtt tgcagagaac gcatattacc atgaactcgc ccgcgagttg
     3001 cgtggtttcg ggtatcgcat tcgcaatctg ccacgcggtg atttccatat agaaggaatt
     3061 ccggccgaac tgtgcgaccg cttttccaag cgcgacgcag aaattgataa ggcattggcc
     3121 aagctgctgg ccgacaaacc cgaactggcc ggttcaaaca tcgccgattt gcgggcgcga
     3181 ctggcaacag agaaacgaac gcggaagcaa aaggatctca gccgtgatga attgcggtcg
     3241 ctctggggcg cgcaactaaa tcaagcggac cgggaagttt tgcgtggttt gacgacaagc
     3301 ccgagtgatg atgcaaaacg acgcgcgtcc atttccgttg tcgaggcagt gcaatgggcg
     3361 gaggagcatc tgtttgaccg caactcggtc gtgctggagt gtcaagtgtg gcaagaagcg
     3421 ctggggcggg cgcgcggcga agatttctct ctggctgaac tcaagcagct cactgaaagg
     3481 cgcggttaca tccgtgatgc ggctcgtcct ggtgaattaa ccaagcgcga cgtgctgttg
     3541 cgtgagtggg agattgttca gaccgccaag gaaggcgtcg gcgactgctg gccgctcgtt
     3601 ctgaatcctc agcctgccaa cccaaagctt gatgacgagc agcgcaaggc gctcgatgga
     3661 ttgcttggct cgacgaatct ggtttcgatc tttcgcggtg gtgcaggcac gggaaaaagt
     3721 ttcgtgctgc atgagttggt tcagcaaatc aagcaatcgg gccgtccggc ggctgtgctt
     3781 gcgccgcaac gtcagcaagt agtggacatg gagaaggctg ggtttccatc gccggcgacg
     3841 gtggcgagct ttctcaccaa agcggaatta ccggagagag ccgtcatcgt cgttgatgaa
     3901 gctggccaga ttggtggaca gcaaatgctg gcgttgctcc gactcgccaa ggaacgaaat
     3961 gcccggatcg ttctttcggg tgacacgcgg cagcacggtg cggtggaagc atcggatgct
     4021 ttgctcgcca ttgagcgtca tgccggcatt aagccggtgg agttgcacaa gattcgccga
     4081 caaggccccg gcctcgctcg cgacaacagc gaacgcgcca gcatcaagcg gtatcgcagt
     4141 gcggtcgaag cggcagcggc gggaagattg gcggactcct tcaagcgtct cgaccagatg
     4201 ggcgcggtcg tcggttgtgg cctcggcgaa caagctgaca agcttgctga ggaatatgtt
     4261 tgtctcgcgg aaaaaagcgc atcagccgtc gtcgtatcgc agacttgggg cgaggtgcat
     4321 cgcgtgaatg cgcgtgtgcg cgaagcacta aaagctaagg gactgctggg cgcgagcgac
     4381 acccccattc aagcgcttga acgcgtggac ttgaccaatg cgcaaaagcg tgatgaacga
     4441 ttctatccgc cagatgcggt catcgtgttc aaccagaagg tgcgcgaagc cgaggtcgga
     4501 tcgaaaggca aactgtcggg aattctcaag accgggctac tggtggaagt cggcggacga
     4561 tttgtcaccg tctcaaacaa gatgctcgac cgcatttcgg tctgtcaggc tcgcgagatt
     4621 gcagtcgctt ccggcgaccg cctgcacctg aaggccaatc gccgactgtc atccggcgga
     4681 agggtcacaa acggagagct ggtcgcggtg aagtcagttc gtggcgatgg cagcatcgag
     4741 ctggccgatg gccgagtctt ggacgcgaac ttccgcgaat tcctggcggg atacgccgtc
     4801 acgtcctacg ggtcgcaagg caagacggtg gattatgttc tgttttccga ttcatctatc
     4861 aaggcggcaa cgcatgcaca gcaatggtat gtgacgatct ctcgcggtcg gcgcggcatc
     4921 cgcatattca ctccggacaa agagcagttg cgcgagaacg tcacgcgctc gggtcataga
     4981 ccattggcgc tcgaactcgc gactggtcta actcctcgtc gtggtgtgcg cctctgggat
     5041 cggctgcatg gttacatgct gcgctttggc cgtcgtgccg ccgacaactt ctgtcgcctg
     5101 aaaatttctc gtcatcgtca tcaacccatt gaaacccatg agcacaaaaa cacccgaatg
     5161 ttgggcgagc gacccgaacg ctcgcgcggt caaaatcgaa gtatccgctg aacaaagcct
     5221 cctgctgccg ttcgatcaat tcgccttcgc cgaactcaag aacgagggca aggagcagcg
     5281 gctgcgtttg gttttcgcca ctcatgaagt cacacttcgc ggccacactc taagacgtat
     5341 cgagaccgcc atacagcgca tggaactggc gttcctcgga gcactgcccg cccatcagcg
     5401 ggcacttgtg accgaaggac agccgttgat cacggcgatt gacgtgagtg tcgctgctga
     5461 aaaagaaaaa ccgagcccag actaaacgcc cagcacttga gccgcctcag tcatcagagt
     5521 gtgatcaaag gcgctggcgt aggtgtccaa cacgcctgcg gtcaggcgca gttttcgccc
     5581 cgtagcgcgc caaccagcca cggcggtgaa cacctcgcgg aggattcgct tcgcttcggc
     5641 gggcttgagt ccgaaacgct cggcggcggc aagcgcgacg gcgatagtcg gttcttcctg
     5701 ttcttcggtg atggcggtct tgcccatgtg aacgcggtca acttcgggca cggggttaag
     5761 gtcataggcc ggggagagag accagcgtcc cggatgaaac atcagaaagc catgattgcg
     5821 cagatggtcg tcgtagttgc tggccaaaag tgaaaagacc agtcgccgca caactcgcgc
     5881 aggtcgccgg gaacatcatc gccgaactgc ctgatgccgt ccgcaagcat ggtgtaagcg
     5941 cccgcctcgc cttgaggcag accaagcaag ctggctgccg aaatgaaagt aacacgttgg
     6001 tggccatttc ggtcgaagcg cgtgatgact gcaacacttt gcatgcccac cgccacgcgt
     6061 tgatgttccg cgactcgaat acctgcttgt gccgccagcg tcagggcaag aatttcccca
     6121 gcgccaatgt cgcgcgtgtc atccggtttg ggaaattttg cgatcgccag tcggtcatct
     6181 ggcaagctga cggccgcctt ggggcgcgcg ccgcccaagg gtgagcctgc tccgagtagg
     6241 aaacgcaaat cctgtgcagt ttccgtttcg ccatgaatgg cgtccgtagc gcgcaacaac
     6301 gcggctaact tcaccaatgg cgggagcttg ccgctcgtcg gggcaagaaa cggaccttcg
     6361 gtattgattc ggaagcgcaa tgctccgatg cgggccgcgt cgtccaaggc gagcacgtaa
     6421 tcaatatccc gatacggctt ctgcgccatg acctttttgc gcaccgcccg ttcaatcaac
     6481 acccgtcccc accgatctgg accgcaatcc tgaatcgcac caaacaatga tttgccgtgt
     6541 tgaacgccgc gctgcaacgg caacgaggtc ggatcaatcg caaaggcatc ggggcgttgc
     6601 agccattcgg tggcatactc gaacgacact gaggcactgc gctcggcgac atcgagccgt
     6661 ccaaccaaat ggctttcacc ctgccaatct acatggactt ccaagctcat gattttttgc
     6721 ggcgaatacg tttcggcagc cgttgctcat ccagactgag ggcgacagca tccgaggcgg
     6781 gcgcggccag attggccagc cggtcatgca ggttgagaat gaacgccgcc gtgaccaatg
     6841 tgccgagtgc caccgtcgga tcgccgcgct ccaatcgcca gagcgtgttg cgtccgacaa
     6901 acaggcgggc ggccatgtcg ctggtggaaa ttccacgctt ccgccgcgct agggcgaggt
     6961 cgcgccccag ctttcgcagg gcgtgagcgg cgggtaatgg aatggaactt atttttggca
     7021 cggttttagt gtcctatatg cgggacattg tgtcaacaga tgccttctat acgaaacaca
     7081 atctcttgat tgcccaagct caaaagccgg tgcgcccgcc tgctttcggc aaaaatgggc
     7141 ttccatattt aggaagccgt tgtcggctag gagtagaaat aacccaagga tcgcaacgac
     7201 tgatggctcg gattgccaat gacgatgtgg tcgagaactt cgattttcag aagctgaccc
     7261 gctcgaatga ggtcacgggt gattttgatg tcggcttcgg acggggttgc ttcaccgctc
     7321 ggatgattgt gcgcgatgat gatggcggcg gcactcgcca tgatggctaa acggaatacc
     7381 tcccttggat gacagagaat ggtatccata gtcccaatgg acacgagata gtggcctttg
     7441 atgcgacggc gggtgttgag gatgaacacc accaaacatt cacattcaga attgaagtag
     7501 ggatgagtcg cgatatgcgt tttccaatag gcggctgcct gatccggcgt ctcgcactgt
     7561 tgcatggtgt cgggtgtggg acattcccgc agggatacga ttttccattc ctgtggttgg
     7621 gatggaaatt tgatgggttt gacgattttg ttctgcggtt gcagagcggt ggtggggcat
     7681 ccagtggtga tgaggttttc cgttgtgttc atgttttgcg tttcgttccg aaggagcggg
     7741 actgctctct tcaccaccgg accgggagag cagccccgct cctgacgaaa cgcacccgtg
     7801 tctggccgtg ccggcgggcg gcggagcatc agcaacgaag tgacagcgga cgaaaagatg
     7861 tttcggccgc tggcgcgagg ttgcgcggag caaccagagg cgcgggcaga cacgggatga
     7921 acacgacgga aaatatcagc gccgtatggc agtatacccg aggttttcga gtgaaatgag
     7981 tgccagcaaa agtgccagca gaattgaggg tttcagcgct gattttcggt ccaaaaacac
     8041 gaatatccca aatcggcccg ttaatggtcg caaaccgacc ctcgcctcca atttttggtc
     8101 gtttttgacg acagaagtgc cagcaaaagt gccagcaact caccagtaaa tgagttcgca
     8161 cagatttttt tgtgagcagt aactgagttt tagcaggctt gacgacgagt gcgttttgac
     8221 cttattttat cggcgttttc gtagcctgtc attcgattga gctgtgaatg cgggagttgg
     8281 agacggatgg agcagcgcgg caaaaatcgc ctgaattcgt tcgattccga ccctcgcctc
     8341 catccgtaat gtcggaagta gaaggagtta agcagggcga aagagccact gccagtaaac
     8401 tgcgaacttc ctgcgctgat accttccggc acctccgata gctccacacc tatccgtcgt
     8461 gcaaatcaac agcgtcgttg ggggatttgg ctgcgccgag tcgttcgtca agcgcgtctc
     8521 accagtacgc tgatatgagg ctccgcgcgt ctctccgttg cccccgccag tgaacttcgg
     8581 aaccaactga agttcagcac aaacatggcg acgccagttt tcaggtattg acccattctg
     8641 tatccccatg agtatcagcc tcgcgcgtgg tttgcttttg ctttttgagg ggtacttcat
     8701 actctctggt catttatgga acgtctgcct ggttttcgcg atttttatcc cgaaccactg
     8761 ccgcaacccg gcttgtggag tgcgaatgca cgccaatata ttttcaatac ttggcgcagc
     8821 ctggcgcggc gctacggttt ccacgaatac gatggtcccc cactggaacc actggaactc
     8881 ttcaccacta agagcggcga ggaaatcgtc ggccaactct accatttcac cgataaaagc
     8941 gaacggcagg tttctcttcg cccggaaatg acgcccacgc tggcgcgcat gatcgccgcc
     9001 tgtgagcggg ggtacaaaaa gccgttcaaa tggttcgcct tgccgcaact ctttcgctat
     9061 gagcgccagc aaaaagggcg cttgcgcgag cactttcagt tcaacgccga cattgttggc
     9121 gaaaaagacc ctgccgctga cgcggaactc attgcactct tgattgatac gctccgctct
     9181 tttggattaa cgcacgagga tttcgtcatt cgcctcagca gccgcaacgc ctggcaggtg
     9241 tttttccggc accggcacgg cgcggcaacg caggaatacg ctttttatca aatcattgat
     9301 aaattggagc gcgaggcacc ggagcaaagc aacgaaaagc tcgaacgcct cgggttttct
     9361 ctggcggagg tgtcttcctt tatcgcgcaa ggcgagccca cggccgagtt gacagccata
     9421 ctggccaatc ttgccgctcg cggactcagg gattacgtga aggttgatta ccacgtcatt
     9481 cgcgggctgg cttattatac cggcgtcgtt tttgaggcgt tcgaccgcaa aggtgagttt
     9541 cgcgccatcg ccggcggcgg ccgctacgac aacctcgtca aacttattag ccaggggcgc
     9601 gtggatcttc ccgccttggg cttcggcatg ggcgatgtgg tgctgctgga actgcttcgg
     9661 gcgcgggcat tgttgccaaa gtttaccgct gcgctggacg tcttttgcct agtcgaagat
     9721 gagaatctgc gagtcgaatt cctgaaagtg atccacgact tacgggctgc cggtctagct
     9781 gtggattatt cgctgaccgc cgctaaacca gatcggcagt tcaaacgcgc gcaggaactt
     9841 aacgcgacgt acacgctcaa actggaacga gatccggcgg gcatgctcct ggcgcggatc
     9901 aagaacctcg aggctcggca ggaaagcgta gttctccccg ctgaggtcgt cacttggttg
     9961 aaggggcgcc aaggccagga agggtttcgg ggtgatcccg caaaggcctg accgctacca
    10021 acttatttca cgcccgtaaa aaacgaggcc acagccacgc ttgctaacga ccaggacgcc
    10081 gctaggattt cacgcccctg gacgcagtgg cggcagtgcg ggtttctttt tctttgacca
    10141 gggttgctcc cttgccgacg cgcttgcgca ggtaggtgag cttggcgcgg cgggtcgcgc
    10201 ctttgcgttc cacttccacc ttcgccacgc gtggggaatg tagcggaaat acccgctcca
    10261 ctccttcgcc atagctaata cggcgaaccg tgaacatttc attcaaccca tgcccgcggc
    10321 ggccgatcac cactccggca aagacctgga tgcgctcttt gccgctttcc acaaccttgg
    10381 tatgcacccg cacggtgtcg cctacgccga aatccaaagc gtctttgcga tattgtgccg
    10441 actcaatctt atcaagtaat gcctgattca taaaatgtct ttgcgaacag gtccggtcgc
    10501 cgttcctggg ttcgccgtcg cgcctgttct gcacgccagc gggcgatttc tgcatggttg
    10561 ccggatagca acacttccgg gactttcatg ccgcggaact ccgcgggccg cgtatattgc
    10621 ggatattcga gcagttcctg gctgaacgaa tcttcccggg cgctgtcttc atcgcccaaa
    10681 actcccggca gcaatcgggt aaccgcgtca ataataacca ttaccggcaa agcgccattc
    10741 gtcaaaacgt agtcgccaat ggacaactcg tcatccgcca accgttcacg cactcgttcg
    10801 tcaaagcctt catagtggcc gcataccatg agcaaatgct cccggcttgc cagttcccgc
    10861 gcgatcgact ggtcaaatcg ccgtccggca ggcgatacca gcaggacgca tgtattctca
    10921 catgccaggt cttccacggc ggcaaaaagc ggctccggct tcaataacat gcctggcccg
    10981 ccgccaaaag gccgatcgtc caccgtctta tggcgatcaa gcgcgtaatc tcgcagatta
    11041 tggagcgcca actccaaccg ccccgcttcc cgcgctcgcc ggatgatgct ttcgtcgagc
    11101 ggccccacaa acatccccgg gaacaacgtc aacacatcaa tcttcatctt cagcttccgc
    11161 ttcgcgctcg ccgggttggc acccgcaccg gcccctcggc gccgaggccc ggcctttcgg
    11221 ttccaacacc gtcaggtggc cggccgatct tccacaatct ccagggtgca acgctgccct
    11281 ttccgggcac tcccggccag cagcaaagag cggatggcat tgatcgtcat cccctgacgc
    11341 ccgataattt tccccacatc ctgcggatgc aggcgcaact cataaaccgt cgtgccctcg
    11401 cgttccactg gcgtaatcgt caccgcctcc gggttctgca ccaacccttt gacgacgtat
    11461 tcaaggaacg cttgcatgga gccgacggcg cttgctttgc ttaggccgtt gctgccgccg
    11521 cggcttttgt cgctttcttt ataaagctgg cgacagtctc gctcggctgc gcgcctttac
    11581 tgacccagta ttgggcgcgg tccagcttta gcgtgaactt atcactcttc ttttgcggca
    11641 ggtacgtacc cagttcctcg ataaatcttc catcgcgggg gctgcgtgaa tcagccacga
    11701 cgatgcggta cgcgggtgtg ttcttcgtcc cgatccgctt caaacgaatt ttaacggcca
    11761 taaatcattt tgttctttgg tagcggcgtt cattcggccg cccgccttaa tggggtcttc
    11821 ccaaaaggaa aacagagcct agtagcacca ccgtgacttg gcaagccctg ttttagggtt
    11881 tttctggcaa taaagcagcg gcccccagac aaggcgaacg actgtcttgc gccgctccca
    11941 ggacggacgc acgcgctaaa gacgatttcc tatcacacac cagcgaaccg ccaaggaggg
    12001 aactttgcaa agtgaaacgc gtttgcacga ttacgagaac gccactcagc aggcccggtt
    12061 ctgattgacg aactcgcggc ttgagaatct aattatagag cagcgagctt atgaaaaaaa
    12121 tcgtcctagc gtgtattatc gtggcggtcg gctgccttgg cttgtggttt tgggcccgac
    12181 ccgcttatcg gcggcaccag gagatccggc tggtggaaca agccaaggca tatttgaccc
    12241 aaaaggaata taacaatgcc tcgctgagtg cccgccgggc attgcagata aacccccgca
    12301 acctggaggc atgccgggtt atggccgatt tggccgaaaa aacccattcc ccggacgcct
    12361 tggactggtg gcgtcgaatc gtcgaggcgg agcctagcat cccccataaa cttcaattcg
    12421 cctctgccgc gctgcattca caaagccctc cctttcccct ggcgacccag atcctggagg
    12481 aactgaaagg aaccgcgacc aacctcgccg cctaccacgc gatttcggcc gaactggcac
    12541 tccggctcaa gcgaaccacg gtcgcagcca aacacctcga acaagcctgc cggcttgaac
    12601 ctacgaatga attgcaccag ctaaacctcg ccgtcctgca actcgaatca accaaagccg
    12661 gcgttccgtc tgcggcgcgg gtgacgctcg aacgcttgcg tgccagcacc aatgtagggg
    12721 atgtcgcctt acgctggctg gtggcggaga gtctcgaacg aaacgatttc tcccgggcgg
    12781 cccgattttc ccggcaactt ctggcggatc cacgggtggt gatgggtgac cgtttgcagc
    12841 atctcgccat ccttcgccaa tcgcaaagcc ccgggttcaa ggattatctt cgcacgcagc
    12901 aaagaaatgc caccaccaac gcggcgcaag tctatgcgct ctcgacatgg atggtcgagc
    12961 gcggcctggc cgatgacgcg ctcacctggc tgctggcctg tccggccaaa ctgcaagctg
    13021 aacaaccggt tcttctggcg gtggcggatt gctatatggc caggaaggat tggcacggcc
    13081 tcgatcaggc cctttccgca cagaattggg gcgaccgcga attcctgcgg tttgcttttc
    13141 ttgcccgcgc ggtgacggaa ctcaatcaga aactggcggc tgacgcccac tggcgcaccg
    13201 ccattcgcaa tgccggggat cgcctggggt ctttaaccac tttgctgact ttggccacca
    13261 agtgggggca ggagcaggcc cgggaagatt tgctttggcg aatcgcgcag cgcttcccca
    13321 gggatcagtg ggcgctgcgc gagctggaac gaacctatac cctggccggc aacacgctgg
    13381 ggctgaacaa ggtgtactcc agcatggcca actatgcccc gcaaaacttc gttgctcaaa
    13441 acaacctggc ggccacctcc ctgctgctca aactgaacct gccccggacg cacgaactgg
    13501 cccgggaact cttcacccag caccccgaac aggccgtgat cgcctccacc tacgcctatt
    13561 cgctttattt gcaaaaccga acccgggaag gcctggctgt gctccaaaag ctaaaacccg
    13621 aagacctcga aaacccgtcc gtagcgctct actatgggat cctgttgacg gccgtcggcg
    13681 agggcaacaa agccagccct tacctgcgca tcgcgcagga ttccagcctg ctgccggaag
    13741 aaaaaatact gctggccgaa gcgctcaaaa ggcccgcccc gaattcctga cggccatccc
    13801 cggcgatcgt tcgccgggtt tcaggagcaa ccaagacttt cgccgcactt gaggcaccga
    13861 tagcaggcgc cgctgcgcac agtcacgttg ccgcagttgg ggcaggctgg cgcgtcctgc
    13921 tgaaaatggg ccaccagttc attgattgtc ttggcaggcc gcatggccgg ggcgggttgc
    13981 gccggcgaat ggcccttgcc gttgccatcg gccccggatt tcagctccag gatctccgtg
    14041 tcatccgcaa ggggcagttc gcgcacgggg cggttcacct ttttcttctc cgcctcatgc
    14101 agcccgggaa tagccaattc cggctggttc tggttcgaag tatgggcttc gcggaagccg
    14161 gggatgaact ggatcgccat ccagcgaaac acataatcca tgatggatgc ggcgttgcgg
    14221 atgtcgcggt tcttggtgaa tcccgacggc tcgaatcgca catgcgcaaa cttgcgggcc
    14281 agggtttcca gcggcacgcc gtattgcagc gccaggctgg tcagggtgcc aatgccatcc
    14341 atcagcccgc caatcgtgga gccttccttg gccatggtga tgaataactc tccgggctgg
    14401 ccggactcaa acatccccac cgtcagatag ccttcatggc ccgcaatatc aaacttgtgc
    14461 gtgatggccg tgcgcgtatc cgccaggcgc cgccgcagcg gcttgccgga ctgcgtccgg
    14521 agttgcgtga tctcggcctc cagttctttg atgcggtcct ccagcgcgaa aatgctgccc
    14581 gtttcagctt tgccttcgcc ttcgcccagg cgcttcgtgc tgagcggttg agaacgcttg
    14641 gaaccatcac ggtagatggc cacacacttc agccccatct tccaggcttg cacgtaggca
    14701 tcccggatgt cctcgatggt gctttcattg ggcatgttga cggtcttgga aatggcgccg
    14761 ctgataaacg gctgcgcggc cgccatcatt ttcagatgcg ccaggtaagg gaggctccgc
    14821 tgcccgcggc tgggcttgaa ggcgcagtca aagaccggca ggtgttcggg gcgcaacccg
    14881 ctcgtgatgg tttgcgcgcc ttccacgaca tcttcaatcg tatggtattg cgtgatatgg
    14941 ccaatgatcc gttcggattc cgcggcgttg tatcccaggc gctgcaacgc ttccggcacc
    15001 gtgcggttga cgatgttcaa gctcccgccg cccgccagca gcttgaattt taccagcgcc
    15061 aattccggct cgatgcccgt cgtatcacag tccatcgaga aggcaatggt gccggtcggc
    15121 gccaggaccg tcacttgcgc attgcgatag cccgtcgcgc ggccgcgggc cagcgcttgg
    15181 gtccaggact ggcgcgcttc ttccttgaga taatcaaact cccggctcgg ctggattttt
    15241 tcgaccgcgt cgcgatgctg ctggatgacc gcgagcatgg agtccacatt gtccgctgcc
    15301 agcggcgccg tcacgtgcgc gcagcgggcg tcgtgataac ccttgaacgc gcccataatg
    15361 gcggcaattt cggccgattg ctcatacgcc cgcccggtca tgatggccgt aagggcgccc
    15421 gccagcgcgc gcccctcgtc cgagtcatag ggcagcccat agctcatgat gagggagccc
    15481 aggttggcgt agcccaggcc gagcgtgcga aagatatggg aattctccgc aatttgccgg
    15541 gtcggatagc tcgcgttatc aaccaggatt tcctgcgcgg tgataaaaat ccgcacggcg
    15601 gccttgaagg ccgcgacatc gaaaatcccg tccgcgcgct tgaacttcac caggttcagc
    15661 gaagcgagat tgcaggccgt gttattgatg aaaacgtatt ccgagcaggg attggtggaa
    15721 tggatcggct cggtgccttt gcaggtgtgc cacttctgaa tggccccgtc gtactgcagc
    15781 cccgggtcac cgcagaccca ggtgccctcg gcgatcttct gcaacagcag gcgggcgtcc
    15841 tttttctcca gcggcttgcc ggtggtgacg ctgcgcgtcc accattcccg ccccgcttcg
    15901 gcggcttcca tgaacgcgtc gctgacgcgc acggaaagat tctcattctg gtacatcacg
    15961 ctgccgtaag cgtcgccatt gtaggacccg tcatatccct gctcaattag cgcccacgcc
    16021 ttgcgttcct ctttcatctt ggcttcgatg aactcctgga tgtccccgtg ccaatcgcgc
    16081 agggtgttca tcttggccgc gcggcgcgtc ttgccgcccg acttgaccac gttggccacc
    16141 tggtcataga ccttcaggaa cgaaagcggc ccgctggggc gcccgccgcc gctcagcttc
    16201 tccttgcacg aacggatggg ggaaaggtcc gtgccggtgc cggagccaaa cttgaagagc
    16261 atcgcctcgc tgtgcgccag ctccatgatg gattccatgt tgtccgcgac ggactggata
    16321 aagcaggcgc tgcactgcgg agattcgtat tgggtcgccg cccgctccgc ctggccggtt
    16381 ttcggattcc acacccaatt gccgcggctt gagctgttgc caatgtgata ctggtggtag
    16441 agcccgacat tgaaccagac cggagagttg aaggccccat actggttcag gcacaaccac
    16501 gtcatctcat cataaaaaac ctcgccctcg gcccgggtaa aataaccgtc cttgaccccc
    16561 caatccgcga tagtgcggca gacgcggtgg atgagctggc gcaccgaagt ttcccgttcc
    16621 ggggtgccct gttcgccata gaaatacttc gagacgacca ccgtggacgc cagcaaggac
    16681 cacgccttgg gcacctcgac attctcctgc cggaaaatca ccttgccgcc gtcgtcggtg
    16741 atttccgccg tccgccgctc ccactcaacc tggtcgaacg ggctgacctt gggatcgctg
    16801 aaccaccgct cgattttgag cgttttcccc gccgcggcct tcgcgcgctt tttgggagtg
    16861 cgggcacccg tcaccgctgc ggcccggcgg cgtcccgcag gacgcgcctt ggacaaacct
    16921 tgttcgttgg caactatcat ctttttcctt ctgcgttgcc ggttgcatcg gctgagcccc
    16981 ctcagcaaag cacccggcga ttaggttcaa attcatcaat caggcaggca atccagctcc
    17041 gactcgcgac cctgtgccta ccctattagt tgggtaacac tctgaactct actaccacgg
    17101 gttggggaaa caagccattt ctgcaaaaaa agtttttcgc cgttttcctg aggaaaatca
    17161 cccaaattac ccccctgaag ccgccccgaa acgcccattt cccggcgccc aggcccatcc
    17221 gcaaacaaat tgcgccccct aacccgcttt ttgcctgccc gccccccgaa acttccgcac
    17281 cggcgcaatg gttttggggc gcggtttggc gacgataatg atcgggcagc cctcataacc
    17341 gaaggcctgc cgcatctggt tcgccaggta tttcttatac ggctccgaaa acaactcgtc
    17401 ccggttcaca aagagcagaa aggtcggcgg cgcctgccgc acctgggtcg cgtagaagaa
    17461 cttcaggcga tacccctggg cgctgaccgg ctgccgctgt tcgacggcat cgcgcagcgt
    17521 gcggttcaga atggcggtgg gaatgctctg ttgcaactgc gccgccacgt agcgcaccgc
    17581 ttcgagcagg cgatccaggt gaaatccgga cttggccgag gtaaaaatga ccggcgcata
    17641 atcgagaaag aacaagcgct cctgcaccca ctgcccaaac tcgccgagcg tggtcatcac
    17701 gttcggagcc gtccggccct tcgggttctg ccggcggcga atctcctgcc gccgcgccgc
    17761 gcgaacggcg tcatccatca ggtcccactt gtttatcacc acaatgcagg cccggtggtt
    17821 ctccacaatg cggtcggcga tcttcttgtc ctgctccgta attccagcct cggcgtcgag
    17881 gaccaggatg acgatatcgc agcgggcgat ggattcctcc gcgcgcttga cgctgaagaa
    17941 ctcgatcgaa tcgccgacgc gccgggtctt gcgcagcccc gccgtgtcaa tcaacacgta
    18001 ggactgccgc acgccctcgg tctcgacctc gaacggcaca tccaccgcat cccgggtggt
    18061 gccgggaatg gggctcacca ccacgcgctc ggaccgggtg agcgcgttga tgatggagga
    18121 cttgcccacg ttggggcggc ccacaatggc gagtttcagg gcggcgggac gcgcgccgcc
    18181 gggcgcggag aacgcggccg ttcccggaac ctcgcccggg ctttccgccg caactcccgg
    18241 cgccgccggg ctggccccgc tttgccgccg gctcatcgcc gcctgcatca gcggttcaat
    18301 gcctttcccg tggatcgcgc tgaccggaaa catttgcggg aaacccagcg ccgcgaactc
    18361 gggcacgccg ctctccgccc gatcggtatc cgccttgttg acggccacca gcaccggctt
    18421 gccgctccgc cgcaaccggg ccgccgcctc gcgatccagc ggcagcaagc cttcctgcac
    18481 gttcacgacc agaatgatga cgtcggcggc ttcaatcgcg agttcgacct gctccagcgc
    18541 cgctttgacg atgacgtcct ccgctttctc gcggcgcagc aggccgatgc ccccggtgtc
    18601 caccagcgta aacggatgcc cctgccattc cacctccgcc gcaacccggt cgcgcgtgac
    18661 ccccggctga tcatgcacaa tggcaatgcg ccgccccgca atgcggttga acagggcgga
    18721 cttgcccaca ttgggccgcc cgacgatggc gatcagcctg ctcatgactt cggaaccggt
    18781 ctcataggct gcaaacggta ggccaaagtc cctgcgcaag ccaccacaaa tccgttcctt
    18841 gacgaagcgc ggcgccgcgc taggctgggg ccgaacacgg gatagatgct ctactttgcc
    18901 tatggctcca atatggatcc ggcgcagttc gtccgccgct gcccctccgc gtgcttcgtg
    18961 accgtggccc aactgcccaa ccaccagctc gcctttaccc gcctggcccg ggaccggggc
    19021 tgcgggactt gcgatgggat tcccgcgccg ggcaaaaaca tctggggagt cgtgtttgcg
    19081 atttccaaag ccgacctgca gcgcctggat gaaagcgaag gctaccgccc cggacggccg
    19141 ctggcggaca acgcttacgt gcgcaaacaa cgccaggtct gccgccctgg cgacccgggc
    19201 aaacgcttgc gggtctggct ttactttgcc aatcgccagc cccatccgcc cctgcccaac
    19261 gccgcctaca aacagcagct cgtcgaagga gcccggctct ggggactgcc tcccgcctac
    19321 cgggaggagc tggcgcgaat ccagaccgcc tgacttgccc cgcgggccga tcccgcttgg
    19381 attggcggtt cccggcgcgg cccgcaattt tcccgcgccc gggtgaacga ggggaaaccg
    19441 cggccgcggc ggcatcgctt gcgccattcg tctgcgccgg ccggccaccg gctttttcgc
    19501 gcgcccgcgg cgcctgccgg cgccaggccg ggccggccgc caaaacaggt tattgctatt
    19561 tttatcctca tgtttgatgg tataagggcc gtcacaccag cgcgtgaaaa taatctcaat
    19621 cactatgggc cgaatgcgat atcccgtcat ggggacgggc cagccgatgc cggcaagcat
    19681 ccccaatgcc gccggggccg ccggcatttc ccgccccacc ggcctggtga caaacctgct
    19741 ccgcttcggg ccatgaggga gtttgcgtgg atcactgacg gcccggaggc cgcccgggtt
    19801 ggccgtcgca acggagaatg acagaaaaag agcttaagcg ttcggtgctg acggtcaccg
    19861 ccttcgcgtc ctttctaacg ccgttcatgg ggtcggcggt caatttggcg ctgccttcca
    19921 tcggcgagga attcagcctc aacgccgtca tgctgagctg gatcatcaac agctatatgc
    19981 tcgcgatttc gatgctgctg ctcccggcgg gaagggcggg ggacatcctg ggccggagaa
    20041 aggtgttcac cgccggcctg gtcatcttca ccctgaccat gctcctgctg gccatcacgc
    20101 cggatatcaa atggctgatc ggggtccgca tcctgcaagg catcggcggg gcaatgatgt
    20161 tcggcaccag catggccatc ctgacctcgg tttttcctcc cggggaacgc ggccgggcca
    20221 tggggatcaa cgtcaccgcg gtctatatgg ggctggcgtc ggggccgttc ctgggcggaa
    20281 tgttcacccg ctacctggga tggcgaagca tctttgcctt cctgataccc ctgggaatcg
    20341 ccagcctgct cctgatccgc agcaagatga aactggaatg ggcggaagcc aaaggcgaga
    20401 gcttcgattg gcccggctcc gtcctctatg gcctctccat ggcggccctg gtctatgggt
    20461 tttcaaaact gccggcggcg gccggctggg ttttaaccgg cgccggcctg gtgctcatgc
    20521 cgctgtttgt catccgggaa aaaagggcgg tgcatccgct gttcgacctg acgctgatct
    20581 cccgcaaccg ggtctttgcc ttgtcgagcc tggcggcgct gatccattac gccgcgacca
    20641 gcgccgtcgg gttcttcctg agccttttcc tgcagtacat caagggcctg ggcgcgcgcg
    20701 aggccgggtt cctcctcatg tccatgccgc tgaccatggc gctgatgtcg cccgccgccg
    20761 gaaaactctc cgaccaccat aacccgggcg tcctggcctc catcggcatg gccatcaccg
    20821 ccctgggcct gatcctgctt tgcttcctgg tggaacagac ctccgtgaca tttatcgccg
    20881 tcatactggt ggtgatgggc gcgggcttct cgctcttttc ctcccccaac tcgaacgcca
    20941 ttatgagctc ggtcgagaaa cggcaactgg ggaacgcttc cggcatgttg ggcaccatgc
    21001 gcaacgtggg gcagacgctg agcatggcca ttgccctgat gctgctggcc atctatatgg
    21061 ggcaggaaaa aatccacccg ggcaactacc cgcagctcat gcgctccatg cggaccggct
    21121 tcatcgtgtt ctccatcctt tgcttcgccg gcatattcgc gtcgctggcc cggaaccagg
    21181 gcctggccaa caaccgcgga aaccggcgtt cattctgaag ccccggcgga cgccagccag
    21241 ggcgcgccgc ccccgcgcca ccccgcgcat cgtcacggcc ggtcaaacaa gtaccagtcc
    21301 gccagccggt gcgcgaactc cgttatcccg gcctcgtcgc ggttggtgag aataatgacg
    21361 gtgaactgct gctcgggaaa ccgcaccagg cgcgtggaaa agccgatcgt gctgccgcga
    21421 tgccagattt cccgcgcgcc gcgatattgg ctgatatacc agccaaaacc atagcgggtg
    21481 tccggcaaat ccgtggccgg gcccggggca aaagcctgct ggagcaattt ccggctcacc
    21541 agcttgtccg tgtagagagc ctgatcccat cggtagagat ccgccaccga ggaataaatg
    21601 ccgccgtcgc ccagcacgga actggtcagg ctctggtcgg ttcgcttaaa acctcccgcc
    21661 gcggccgagt agccatacgc gcggttggcg accacggaca cgcccccctc gtaggccagc
    21721 gtccccttca tcttcagtgg ccgaaagatg ttctgctgca ggaaccgggg aaacgtgagg
    21781 cccgagcgcg ccgtcacgat ctcggccagc agcgcgtagg ccgaattgct ataacgatac
    21841 ttcgtgccag gcgggaaata cgtcttgtcc tgccgcatca gcaaccgcaa cacatcgcga
    21901 tccagcaccg gcagctccgt ccccggcggg atcaggtctt cgtaatccag cagccccgag
    21961 gtgtggttca acaactggcg cagagtgatc tgccggccgt aggcgggaaa ctccggaaag
    22021 aaatcggtca gacattcctc aaaagacagc ttccggcgat cggccaggat caacaccgcc
    22081 atggcggtga actgcttggt cacggaggcc aggcgataat tggtctccgg gccgcacggg
    22141 attttctctt ccacgttcgc caggccgtag cctttcgcca ccaccacctt cccgcgatgg
    22201 atgaccatca cgctggcgcc gggcgattcc ggctggttaa agttctgaaa cagcgcatcg
    22261 agttggggcg tcaaccgcgc aactcgatcg gatgcggcag gggccgataa cgtcatggca
    22321 gcaatcatca acaggcggat gaacacgccg gcgagtatgg caaacccgac cgggttctcc
    22381 aagcactgct tgccccaact cccgcaaaac cggccaggcc cattgttgcc ggcgccttcg
    22441 ggccaattgc cggtcaaaga gcccaggcac gaaccccgct gagccaaccc gccagccccc
    22501 aatccagcgt ttttcctcca gcaagagctt cccaataccg ttccaacccc ctcccaaagc
    22561 gccgtgtgca aggggtgtac aagggatgcc caccggaggt acataggatt taagcttgtt
    22621 cacccgatga gcatcccttg tacacccctt gtacacgact agctgccagc tatggatgag
    22681 gctggaatgg cgtaagttag attgaaaatg gcgttcggat tggcttttga gttcggaaac
    22741 agggaagtgt ccaataattg gacagtccgg aggcggtccg gatggactgg ctaattacta
    22801 taattgggtt ttgctcacag gttcctgcct gggtttggtt gcgtcgggaa cgggatgccg
    22861 ccgggaggca gcgtttagct tgcgttggcg gaagcaataa aagacttccc agatgccggg
    22921 attcgaggta gggtagggtg ttagcttatt cagagatggc atagccgggc tctcgcgttg
    22981 agctgcatga aactgttaat attcgcccac acaccgccgc cgcatcatgg ccagagctac
    23041 atggtgcagc ttctgttgga ggggttgggc ggggaccacc gccgccggaa acggcgggcg
    23101 gcaggttccg ggccggagac ttccttcggc atcgagtgtt accatgtcaa tgcgcggctg
    23161 tcgcagaagc tcgaggatat tggcgatttg cgggtcagca agctgatcct gctgctgggc
    23221 tattgtttgc aggccatctg gtgccgtttc cgctacggcg tcacgaatct ctattacatt
    23281 cccgccccgg gaaaacggtc ggccctgtac cgggattggc tggtcatgct gctgtgccgg
    23341 ccgtttttca agcgggtgat cctgcattgg cacgcggcgg ggctggcacg atggctggaa
    23401 ctggcggtgt ccatgcgctg gcgcgtgctc acgtaccggc tgatgaagca ggtggacctg
    23461 agcatcgtgc tctcccaata caaccgcgcg gacgcggaga agctcttttc ccgccaaatc
    23521 cgggttgtga gcaatggcat tgcggatccc tgcccggcgt ttgccgggga gattttgccg
    23581 cggcgcaagg cgcgttttgc cgcccgccaa aagctcctgt cccggcagcc cctggaagct
    23641 gacgaagccc ggctggccgg cgcggaaccg catttgatcc aggtgctgta cctggcgcat
    23701 tgcactccgg agaagggatt gttcgacacg ctgagcgcgg tggcgcttgc gaacgagcgg
    23761 ctccagcagc gccagtcgcc ggtggctttc cgcctggtgg tggccggcgg tttcgtcacg
    23821 aatgaggccc gggtcaaatt cgaccagcgg attgcccggc ccgacctcgc cgcctgcgtc
    23881 aagtatctcg ggttcgtttc gggcgcgcag aagaaccagt ggctgcgcga ggccgatctc
    23941 ttctgtttcc cgacgtatta tgagaacgaa aaccagccgg tgaatctgat tgaggcgatg
    24001 gcctttggcc tgcccatcct taccacccgc tggcgttcgt tgccggagtt gtttccggcg
    24061 aattacccgg gcctggttga tattcgcgcc ccggagcaag ccgccgaccg tctcctggcc
    24121 ctgatcacct ccgaaacggg cgaggaactt cgggacattt tcctgcgcca cttcaccctc
    24181 acgcgccatc tgaccgccat ggccgaggcc ctgaggcaag ccgaaacgcc gccgcccgcc
    24241 gccgttcccg ccgcggccgt tcgttcgctg cccggataac ctccggcggc gcgctgcctc
    24301 accggcgccg caacacccgc gccacaaacg cctcgacccg cgcgcccact tcgtcttcct
    24361 gcgtgttgaa catgcaatgc ggcgtcggga aacggcaata atccgagcag ggtttgtagg
    24421 gacacgcctt gccttccagc cactccgagg cggggttgag gggggcgaac cattcagcca
    24481 tttgcgggcc aaacagggtg aaggtgggca cgccgcagaa agccgccagg tggccggggc
    24541 ccgagtcgtt gccgatgaag gcgcccgccc gatccaccag cgcgaacagt tccgtcaccg
    24601 tgcggggggc tgccacgttt gtttccccgg cctttaccca ccattcccgc tgatcggggt
    24661 cgcaggcgat ttgcacggag tggtggttcc ggcgcagccg ggcaaccagc gtgtggtaac
    24721 gttccagcgg ccagacgcgc accggctggc cggccccggt atgcacgaga atcccgcctt
    24781 ccgggttcga ccggggccac gccagcgccg cgcgcggcgg cacctcgatc cccaactgcc
    24841 gggccatcac gcgccaatgc tcgtagcggt gttcggtcgg gtccgggcgg gcgggggtcg
    24901 cgctcaggat catttggctt ccccaacgcg cgaatcccag ccgccgccgg acttgcgcca
    24961 gcagcagcag cacgtggtcc cgcgggtccc agcgcgccga caggccgaaa tcgaagcgct
    25021 tcgcgaccag ccggcgttgc agccggagca tctcccgcca gggccagcgc agcagccggt
    25081 atttgtgttt gaaggcggtc catggcgcca cgaaaggctc cacccgcacc tccggccaga
    25141 agcgtttttg caggtccacc gcgtacggct tggccaccag cgtcaccgcg taggaccggc
    25201 tggcggcttg caggaaggga gtcgcaatga ccaggtcccc caggccccaa agctccatta
    25261 ccagaagcgt gggcttcggg gcgttcggcg ggcaatctgg ttttggcctg gccatttttc
    25321 ggttgtctta aggcggcaga aagtcatggg ctgggaaagg cgattgccac cgcccttcta
    25381 cgtccacccg gcgccacgtt agcccccgcg ccccgccctg taaagcccgt ctccgcaccc
    25441 ttgcccggca actcccgtct cgcgcctggc gggcttgcgc cggggaacag cggtttattt
    25501 cctcaggcgg accagggcgc cgccgccggc gagcagattc agcttgatgc ggcggcctcc
    25561 ctctggtcgg agccaggctc gcgtgggcgg gtggaagact tccagcggtc ccgggccgtt
    25621 cagggtgagg gtggccgcgt tcttatagtt caggttcacc acgaccacgt gcgtggggcg
    25681 gcgggcggat tttccgaaat agcccagcaa ggtttccgct gtcgcgggtt ccgtcgcaaa
    25741 ggcgctttcc ggcggcagtg cctgtccgcc caggggaatg gtcccgcaat ggtaagcccc
    25801 gagcgatttg agcggctgga gttccgtggc gatggccagg aactggcggt tgagctgcgc
    25861 cacggcccag taaagcggca agggaatggt ctgctcattg gggtcccgcc aaagcccggt
    25921 gtcgtagcga aaatgggcga tgccctgcgc gccataagcc agggatgtat aggtgagcca
    25981 gcgcacttca tcttcacccg gggcgcgcca gccctcggcg ggagagtcgc aggtctgaat
    26041 gatgttcaga aaaggctttc cggtttccag cgcggccgcg cggatcagcg cgaggttgag
    26101 gaagtactgc gggccgtcgc tggacttgag gaagtggtag tggtcgtagc tgagaagatc
    26161 gggctggacg atgcggacaa actgccggag gtgttcgcgg tagcgcagcg ccgtgaagtc
    26221 gtcggcctgg aggccggcga agtcctgcgg gcagcccacc cgggcgcgtt cggcggcatc
    26281 gtcgctgact tgaagctgcc cgtcgctggc gtaggtgggc aggaggttga tgtaggccag
    26341 gtgcgccggg tcgcgctggc gcagccaggc ggcgagccgc cccagtttgg gaaacgctcc
    26401 cgcgccgggc tcatccacaa ggtaataggc atagagcgcc ggatgcgagc gcacgcgcgc
    26461 gatgagcgcc gccagcgcct gggcctgcgc ggggtcttcc acgtcgggcg ttgcgatctc
    26521 cagcatgacc cggaggccgt ggcggtggta gagatcgaga tcctcgggcc gctgggtcca
    26581 ggcaaggttc cagccgccgg cgcgaagctg cgcggcggct tgttcattca ccgccgttcg
    26641 ggaaccgggc ccggcccaat aggtgatgat gggagcgccg agctgccagc gcgccgcgga
    26701 ggaccgggcg ggcgccgccg gtgaaaccgc gccgccgatt cccagggcgg cgcagacgac
    26761 ggcgatcagg attgtgcgcg gacgtggttt gggcggggaa gcgggattgg ctttcagggc
    26821 tgcgagggcg ttggggcggg tttgctccat aagcgtttaa tgatttccac ggtgcgctcg
    26881 acgagttcct gcccgccctc ggggccgacc tgcccgctct ggacgatggc gctgtaaccg
    26941 ccgccggcga cggcgcgcgg ggtgggcagg tagccgctcg ggccgcccgt cagctcgatc
    27001 accagcgttt gcaaggccgg gctccggctc ttgatttggg ttccgtagtc ctggaacagc
    27061 tcgaaggggt tggtggcgat ggcaatatcc ccgatccgca ggacatgcac ctcggcggta
    27121 acggtgggcg cgtccccttg cgcctggtag cggtccacgg tggcctggtg ccacgctatg
    27181 cggcgggtgg cgtcgttctt tcccttgagg gcctcgattt ccttgatcgc ttccgcggct
    27241 tccgcgtcgg tcactttgcg cgcgggcagc accaggtccg ccacttcgtg ggcgaagggg
    27301 acttcccgct ggatgtcgct gcgggtcagc tcgctgactt cgaggacctc gcccgcaatc
    27361 cggcgcccga tgtcctgggt gcgcgacagc ccgcgcaatt tgagcatgcg ctcctcggcg
    27421 gccttgcggt agagccagtg gggggagaga tcgccggccg ccccgggcca gccgagcacg
    27481 ccgagttcgg ccccggcggc ggcgcgcagc cgttcgcgaa catcgtgcca gaaatcggcg
    27541 ttaatcgtgg tccggctttc caccacctgg gaggggcagg ggatgttgac gccggccgcc
    27601 aggagcttct tttccgccgt ccaaaagaac agcaattcca ggccgtgatc cgcgccgccc
    27661 tcgatgccgc ggaattcggg cgtgctggtg gcgccgtaca tttgggcggt gccgttggca
    27721 tagacggcgc ggcggttgtg gcccaccacg gcctgcccca gcccccagct tacgccgccc
    27781 ggctgccgct ggttccaggc gcgcacgctg acgtccacca gccgcgccac gaggaattcc
    27841 acgtattccc ggggcggcat taccccctcc ttcggaatgt cgtatttccc ttcttcggta
    27901 accggcccgg tgtgggtgtg cgtggcggtc aggaccagtt tgcgcggatc gaagccgggc
    27961 agttgggcgc gcaagcgttc gcgcacccgc ggcgggatga acgagcggaa cgccacgaga
    28021 tcggcggaga gcaagatggc ctggtcggtc gtgcgatcgc ccacgcgcgc ctcgagggcc
    28081 accgccgtgg cggtgaccgg gttgtccacg ccccgggaga cgcgcaggtt gaactgcccg
    28141 tcgagggcca cgggctggtc cggggtgatg ctgatggcgg ccgtgccgat gcgcaactcg
    28201 gcggcgcagc agttcacagt gacggccgcc gccaggatca gcatggcgga cttcgtgggt
    28261 tgcccccggg ttgcgccggc gtgcggcgcg gcggggttgg aagaatgaat cgcagcagtc
    28321 atacatcgtc taatcccggg cctcaatcgg cgcggacgcg gtagaattgc acgttggcag
    28381 gggacggatc caccacggaa atatcggctc ccgttccgtt ggtggcgccg ttcaggtcgg
    28441 accagttggc gtccgcga
//
LOCUS       DAOPQG010000041        28153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2170_length_28153_cov_16.226707, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000041 DAOPQG010000000
VERSION     DAOPQG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 28153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28153
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_28153_cov_16.226707"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1040..3439
                     /locus_tag="PKI07_06495"
     CDS             1040..3439
                     /locus_tag="PKI07_06495"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC50740.1"
                     /translation="MLFAASSLHRVCRVVSVMIGCAGSLAFSSFSTFAASSVTVAWSP
                     NANPEIAGYRVYYGGASRVYTNQVDAGRANSAVINGLTAGATYYLAATAYDVAGLESD
                     YSVEAVFTNLAVLPPTIVLSAPANGTVFTAPATLHLAADVAANGHSIVKVQFYNGSVL
                     LGEDATPPYEFAWGNVSEGIYDVKARAIYDLIGLVTSPPVRANVYTPPKPPNTPPILS
                     ALADQTLIAGSGPLVVNFQVGDAESAPGKLALAAETSDAKLLPVENIVFDGSGNNRVA
                     TLRPVAGRTGAVEVTFMVHDGEAATSASFWLTVIEKKPEPAGIALVSPVGAANMNSTQ
                     RYVWRPDPAATRYELEVTRDGRLFCDNWYDAINLPADPETANVGVEVAGHSNGNYQWR
                     VRGWSAAGLGPWSSAGTFAVATPRPVVLLTPADQTSLQERRPQFSWEQPVPPAEWFHL
                     CINRNGSRCYDLWLEGTTQWTPSVDLPGGAYTWAVQAWSPAGVGPWSKMASFTAQPAL
                     PGQIALLTPKSSALRGPTLRYHWSQDSAAVSYELYITRDGAVFYDQWLSVTNLSADPG
                     KGSLSVEVPGHAAGSYYWYVRGWNPDGFGPWSRPGSFSVDGASKPLTLLTPTNNAVLQ
                     ERRPEFSWVAASPPAAAYHLYLSRSGTNYYDVRVEGKTNWVPSFNLTGGSYTWMVQAW
                     DASGSGASSETASFTSPYIVPAAIVALTPRGTAGDSATQRYQWQVDAAATRYQLYINR
                     DGSVFHDQWYDAAHSVLDPATGKFAVDVPGHSPGTYQWWVRGWGPEGMGTWSDAVTFR
                     Q"
     gene            complement(3594..3959)
                     /locus_tag="PKI07_06500"
     CDS             complement(3594..3959)
                     /locus_tag="PKI07_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50741.1"
                     /translation="MKKVILIMTTLALGGMSAQTALAGGRHCGGGGWGTAAAVIGGVT
                     AGLIVSHALASPPPCYVAPAPVYYPPPPPPPVVVCPPPAPVVYAPAPAVVVYRPPVCV
                     APPAVRVSFGYGWHRHGCR"
     gene            4056..4985
                     /locus_tag="PKI07_06505"
     CDS             4056..4985
                     /locus_tag="PKI07_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC50742.1"
                     /translation="MKKRSPYRPPVSRERLPRSFKEFTPARHFNPRTFFRELVWKALL
                     TPRTGQTRQPVFPKLNRGQVAITWIGHASFLLQFTNLNVLIDPNFANWLFLLKRVKRS
                     GLSLEHLPPIDLVLLTHAHFDHFHKPTLRRLPHPKIGVMPWGVGDLAQNLGFGRVVEL
                     EWWETFCHRDWKVTLTPSKHWGARVLRDQRRGYGGFVLEHQGRRIYHAGDSAYFEGFK
                     EIKKSCRPEIALLPIGAYHPESFRNLHMGPDEAIKAFRDLRARWLIPMHYGTFRLSFE
                     DMDAPPRWLQQLAVEQGLTHKVRMLVEGVPQVF"
     gene            5178..5633
                     /gene="nrdR"
                     /locus_tag="PKI07_06510"
     CDS             5178..5633
                     /gene="nrdR"
                     /locus_tag="PKI07_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006692645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOC50743.1"
                     /translation="MRCPKCGCQDDKVIDSRASREGATIRRRRECVACGFRFTTYEEI
                     EQAGLMVIKHDKRREEFSKEKLLSGLKKACQKRPISPKVIEDLVEKIVNEITDKYERE
                     VPSEVIGKLVMAGLRTIDDVAYVRFASVYRRFQEATDFVQEVKKLEARP"
     gene            5630..6187
                     /locus_tag="PKI07_06515"
     CDS             5630..6187
                     /locus_tag="PKI07_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50744.1"
                     /translation="MIAPGSDWLFLELDNGERVPCSADMVDVELACSADGLFDAEFIH
                     QVTRAVFHYFKHELGRQSVAAAEFAGAMEKVLRGFTTAAGAAAAPVAAAPELGADLCR
                     LARESGPGRELIFFPRLRAELRRCLGAGPRWLRFSGLRACVKHLAGARRWSRRCQALE
                     GEIVAYLRECLRAEPEPMELVLRVE"
     gene            6203..6547
                     /locus_tag="PKI07_06520"
     CDS             6203..6547
                     /locus_tag="PKI07_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOC50745.1"
                     /translation="MSQTLFEKIIARELPGTILYEDDQVVAFQDIHPQAPVHVLIVPR
                     KPIPRIAAATPEDQPVLGWLLLKAAEVAGRLGLARSGFRLVFNNGPDAGEAVPHLHCH
                     LLGGRPLGWPPG"
     gene            complement(6578..7858)
                     /locus_tag="PKI07_06525"
     CDS             complement(6578..7858)
                     /locus_tag="PKI07_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOC50746.1"
                     /translation="MKAHHLSALGRRTEDPPISWLLAMALGRPQLVSLAAGFTDNKSL
                     PREKVRRLMREILSSPQTGQTALQYGTTAGDPALRQLTSQHLCRQDEQPASRPAYSPE
                     RMIITNGSQQLLYMLTEALCDAGDLVLVEDPSYFVYLSILQSHGIQARGIRMEADGVD
                     LDHLEGVLRDLQRRGELRRLKLLYLVSYYQNPTGRTTRYAKKAAALQLLRRFERAAGH
                     PLYLLEDAAYRELRFGGEDIKSALTLNGARDRVIYTGTYSKPFSSGARLGFGLLPEPL
                     LSAVLRIKGNHDFGSANLLQQLLARTLSSGLYLKHLDGLRQRYAYKARLMREAIERHF
                     PAEVQWQEPAGGLYFWAQLPGRRATGMNSAVFRAALKNNVLYVPGELCYADDPSRRKP
                     NHEMRLSFGGATEDSVRQGIARLGAVLRNRPRGR"
     gene            complement(7946..8791)
                     /locus_tag="PKI07_06530"
     CDS             complement(7946..8791)
                     /locus_tag="PKI07_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC50747.1"
                     /translation="MKRLPRLAKTVIGLLVAGLLIILMFRWLERNLVYHPDRVLTATG
                     AELHRPFEDVRFRTRDGLELNGWFYPADPQSERAHLVVLYCHGNAGNIGNRLEAYEAL
                     LTAGVGVFAFDYRGYGRSQGRPSEEGTYRDAQAACEWLAQRGFARSRIIAYGYSLGGG
                     VAAELAVREPVGGLVLQSTFTSIPAIGAELYPWLPARWINTIRYDTRAKLPGLRLPVL
                     IMHSPHDDLIGFHHAQTNFAAANEPKLFQEISGDHNSPLANRAQYLAGLENFLRQFEA
                     PAPAR"
     gene            8961..10337
                     /locus_tag="PKI07_06535"
     CDS             8961..10337
                     /locus_tag="PKI07_06535"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HOC50748.1"
                     /translation="MSTTVQTALPGGEWSPSSWQSRPAAQQPDYPDPAALRRVLAQLS
                     RLPPLVTSWEIENLKRQLADATRGERFLLQGGDCSESFADCESGAIASKLKILLQMSL
                     VLVQGSKKRIIRLGRFAGQYAKPRSVNTETHDGISLPSYRGDMINHAGFTAEDRRPDP
                     ELLLRAYERSALTINFIRSLIEGGFADLRHPEYWDLGFVAGSAHAAEYTRMVETIGES
                     LRFMETLTGGVLADINRVDFFTSHEGLHLAYEQAQTRQVPHRTGWYNLSTHFPWIGDR
                     TRALEGAHIEYFRGIANPIGLKIGPSITPEQLVALAGVLNPQNEPGRLTLIHRFGVDR
                     IERCLPPLVTAINRAGRCVLWCCDPMHGNTETTRSGIKTRRFEKILQELEAACRILQD
                     CGAHLGGVHFELTGDNVTECLGGASGVTEADLTRDYRTALDPRLNYEQSMEMALLLAR
                     LLSPRRKP"
     gene            10898..14884
                     /locus_tag="PKI07_06540"
     CDS             10898..14884
                     /locus_tag="PKI07_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOC50749.1"
                     /translation="MTGLHAVRGQPVTFPDAGLEAVVREAIPKPSGTITVADMLTLTN
                     LVVASGRGIEDLAGLETARNLWRLELGWNPITSHTALASLTNLLLLSISANDNPDISF
                     VAALKKLRELNLPNNGIKDISPLAGLTDLQRLYLNYNAVTNPAALSALTNLVELVLDF
                     NNVSNLSFVVPLRKLERLSLNNNQVSDLSPLASQTNLLYLNLNWNGVTNPAVLTTLTC
                     LRELTLCGNNLIQVPFLSGLTNLTGLDLAYTSLADMSPLTNLARLEWLNVGENGLAQL
                     PDLSVLSNLRTFMMAGNQITDLTPVTRLPGVWDLHLQRNPFEDLSPLTHCPHLRVLLF
                     YDNHTITNLSVLGAVTNLETLYLGHMYFSDLQQIDFLAGLAGLRGLDLSENYLTDLSP
                     LTNYPSLSWLGLAANRLQHIGPLRNLPTLDYVDLSANYLDLNPASAAWSVIPELQDHG
                     VYVEYNAQQVAPASPRILRQPANRSAFAGDPVTFTVLLENNGGWPEFRWQKDGVDLEN
                     DARYDGVDSDQLILYAVTAAEAGHYRVRIWRDWVETYSAAAELRVVTNVAFADPNLEQ
                     AVRDELGIPVQPLTPADLAGLSVLSAGYRGITNLAGLEAAAYLTSLDLSGNPYLDDFA
                     ALTYLSSLNELAINDAGRDTLDWVANLPTLNLLWAGENFLEDLSPLHALTDLISLDVA
                     GNQLTNIDPLLDLPALGSVNLSLNRLDTDANSAAWNVITNLQARGVTVDYDPQYDAPA
                     PPLITSQPASVVAYLGDNVSFHVEASGSPNYWWLKNGANLCDTERIGGTRDDTLYLDN
                     VQASDTASYRVRIWDEYGVTNSRTVTLRVITQVAFADPRLEQVVRDHLGIPTRPLVPA
                     DVAPLTWLNAWNRGITNLSGIESLANLDMLNLDSNPGLTDLAPLLQLPRLTRLNLNNC
                     GVRDLAFVAALPPLTELHVWGGRFTDLAPLLSQTGLQWLNLGNNPGLADFTVLDHLTE
                     IQYLWLDSVGLTNLAFVGYMPQLVELNLWNNAVSDLTPLAAVPQLVRLNVGANQLTDL
                     SFVGNLPGLERLEVSENPISDLTPLIGLTHLTQLGIGATLATNLAPVATLTNLTELSV
                     RGLGLAHNLSFLAPLTRLQWLGLEDNGISALPAYPSLDRLIGLNLSGNPLADLASIVA
                     RTNLSHLYLNNTGLSDLSALAGHTNLNNLGLADNQLTDLSPLATLPHLRWVSLWNNQV
                     QNLAAFAGLTNLDYVDLRYNWLNTNSGSAAMTIIGLLQGRGAFVEYEPQNLSPGEVVL
                     RAPAWLGGNQFRFTIDGPPGALLEIWRSTDWNHWDSLGFVTNNTGTTVFTDSAAPASR
                     AFYRAQLP"
     gene            15094..16356
                     /locus_tag="PKI07_06545"
     CDS             15094..16356
                     /locus_tag="PKI07_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC50750.1"
                     /translation="MKEGLLSPRRRRWIILVLIFGGIVLNYFDRQIIAILKPTIKAQF
                     HLDDAGYALLTNIFTVCYAGMYPLAGWLVDRWGAGSMMLAGLLAWSSACLGTGLTRTF
                     GQLAFCRGMLGLAEPVAFPAQLRAVTLWFPGSLRATANSLCVAGSSIGAIAAPPLVAW
                     LALKWSWQAAFIIPGAIGLGLAGLWRLLYRDPPAEMMNPVADAAEPGPAEKFAWPQLW
                     RTRSLWGIVLIRFISDPVWYFCLFWLPGYLQEQSGLTLAQTGQVGWIPFLAGGLGGVG
                     SAAWSDWMVKRGLPPVRARKLMLTSMAALGPVCALTPHLPNAAATLAIFSIASAVCLS
                     WLYSLSVVIAEAFPTDNIGSVLGIAAGFGAAGAMLFNYFVGQVMGTLGAANMFIIMAL
                     LHPLAALVLWTMVRREQPKRRATPGPGR"
     gene            16499..17416
                     /locus_tag="PKI07_06550"
     CDS             16499..17416
                     /locus_tag="PKI07_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC50751.1"
                     /translation="MNRRTFIRNLGLGATLPWLGRAALAAPARAAVHTLLSCNIRVAL
                     PEDEATGNGWKARRELCLEVIRAQQPDLICCQEVLREQMDDLTRGLPEFAGFGFEGPE
                     MDARKEGYQGIAKNPIFYARERYELVTAGGFWLSETPHLPGSLSWESARARHVNWVRL
                     RERASGRSFRVLNTHLDHKSQPAREAQMKMILAEAALYAPDFPQLLAGDLNAGAANAV
                     LQLALDAGWSNTRTAAPGPPEEGNTTHEFLGPKYVPKTPGARKRGPIDHILTHGPVTT
                     RAWKIIRDGRDGRYPSDHYFLAVKFTLDA"
     gene            17429..18364
                     /locus_tag="PKI07_06555"
     CDS             17429..18364
                     /locus_tag="PKI07_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC50752.1"
                     /translation="MHRRQFLRCLGLAAALPGMGRATLAAAESTPAAESAPAPAAAAP
                     GGTHKILSCNVRGDLAGDARTGDSWAERREFCADVIRAQRPDLICLQECQQNNWKYLK
                     LRLPEFDSFGLFDPGPVPHPMNTLFFARARYELLSAGGFWLSEQPHVAGSKSWDTAYS
                     RLVNWVHLRERATDRELRVWNTHLDHLGAVAREKQAELIVQAAAVFPAALPQLLTGDF
                     NCPTGSPPAERIRAAGWTDTYAAVHGPKDPGFTFHGFQGPGFATHKLARKLSGKIDFI
                     WCRGPVKTLAAEIIRDGRNGHYPSDHYFISAVVAM"
     gene            18386..19768
                     /locus_tag="PKI07_06560"
     CDS             18386..19768
                     /locus_tag="PKI07_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC50753.1"
                     /translation="MNNPALGSIQEPARNVPVSAAVEVLVVGGGPAGIMAALAAAEQG
                     RQVALVEGRSFVGGNLALGLPILGFLSQKGRPIITGLPQQLIDRLRAQGAASEHQPCP
                     LHVSLTLVEPEAVKSVAMEMLLERGVQLMLYRQFADAVMDGHRLRGIITESKAGREAI
                     LAPVIIDATGDGDVAFRAGVPCEKGDAHGGMQPPTLMFCLAGVDTEKLRRSLADDPQT
                     YRADIIPAEYFARNRQFIVVGLRQRLQQAQADGLTLPTERTILITGLRTGEVWVNMTR
                     VKGVDGTDPQSLSTGEIAARRQIADIQRYLVQYVPGFAQASLAKVAPFLGLRETRRIR
                     GRYVLTREDILGCRRFDDAVAVASYPVDLHHPSDDDCTLEWCGDCYDLPYRCLVPQQV
                     ENLLVAGRCISTTHEAMAAIRVMSTCMATGQAVGCAAGMAVAERIPPSALNVSRLRDE
                     LRARGAYLRD"
     gene            complement(19796..20971)
                     /locus_tag="PKI07_06565"
     CDS             complement(19796..20971)
                     /locus_tag="PKI07_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC50754.1"
                     /translation="MRPANATGSDAPFAPPGPDAGALRPVPRPRTGPWQLVREVLRDG
                     GPGYLQFAITNRCNARCEFCGFAVDQLDPKQRRSVTLAEARDVIDICVRNHIGYLLLV
                     GGEPLAHPDVRAMTRYAAERGIRPMICTNGSLWTDRNMRELAADGLCSVIMSIDAPDA
                     ARHEQSRGLPDVCRKIQRANEVFARLGVQTTASIIASRLIEDYDRLPAFLTALGFRSC
                     TFSYPLRSLGSSYLSFSGSQLVNYSPAELIEVFERIKRMKRRTGFPVVNPRESLTEMQ
                     RHLRGEPEKFGCLGGYKYFYLDWHLNLYRCHFWETPMCRIYDFDRSKLIRDNCTRCMI
                     DCYRDPSVLQFIAVNVSDAWNHLKRGRLLAAARNLCDPRNLTSLRAVWEQRRWIREV"
     gene            21207..22724
                     /gene="cobA"
                     /locus_tag="PKI07_06570"
     CDS             21207..22724
                     /gene="cobA"
                     /locus_tag="PKI07_06570"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOC50755.1"
                     /translation="MKPKGMVYLVGAGPGEVGMLTLRAAEVLARAEVVVAGEGVSPEV
                     LALAPPTAEFIGAGSGGSPAAQSPEEVGRLLAASARAGKRVVRLIGGDPYLFGGGGEE
                     AEWLAGARVPFEVVPGVSALVAGPNCAGFPLAHRQWASQITLLAGTEAPAAVDWAQVA
                     RAPGTRVIALAPERIGPTVELLAAQGMAPETPVAVVGPALRGRHHVWQGTLATIAGVV
                     AREEIRPPAMLVIGGVVQLRARLNWFERRPLFGQRIAITRARAQAGELARQLRELGAE
                     VLEVPTLRFEAPSRRQDLVDALLELNAYDWLVFTSPNGVTRFFEYFFRRFHDLRDLGG
                     ARLAAVGPGTASKLRELHLQVDLVPDEALGVEVARAFSQFESVENLKICLLRAEAANR
                     DLPEALTALGAIVDDVACYQTVPETEDATGAAASLRERGADWVVFTSGSTVRNFHARF
                     DLPALQRQFPQLKAAVIGPETARALAALGVAPACEARPHTLEGLVKVLLAAAAAK"
     gene            22904..24760
                     /locus_tag="PKI07_06575"
     CDS             22904..24760
                     /locus_tag="PKI07_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOC50756.1"
                     /translation="MRRLSSWIKSNSRRRSRQELGRGVALLAGLAWLAPLLAGAASFT
                     ATLDRENVTVGESATLTLRFEGGNPKSIPFPSDIPHLQISDAGTSRNISIVNGQYSAS
                     FLKNIILTPTQPGEFVIPALTAEVGGQVLTTPPLKLTAVKAPTSAVDGAGEQLAFFRL
                     FVPRKEVYVGEILAIELQVCIREGLANGEDILQSFDQFNGCPLKVEGVSLIKTAHAQR
                     RRGQIGNAGYGIATLVTSLSPLRSGPISISSMEVPLTLQIPLPNQRRRDPFDPFGMFQ
                     RLQVEERRVTLSAEPETLEALPLPAEGVPAGFNGAVGSFAMTVTAGPTNVAVGDPVTV
                     KIQLAGRGAFDALALPEQTAWQDFKTYPPTAKVETTDPLGLQGVKTFEQVVVPQNPEL
                     KALPALAFSFFDPEQKRYRTLTQPPIPLIVRPGGPAPVAVAAASARNPAAEAPPPAPD
                     IVHIKPRLGAMAQVAAPLVFQPRFLAWQALPLLAWLSALLWRRRAEALAANPRLRRRR
                     RVAQVVRQGLAELRRHAAGNRSDDFFATLARLLQEQLGERLDLPASAITEAVIDEHLR
                     PRGVPEAVLGSLRELFQTCNFARYAPIKSSQELTAMIPKIEAALRDLQELEL"
     gene            24757..25530
                     /locus_tag="PKI07_06580"
     CDS             24757..25530
                     /locus_tag="PKI07_06580"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC50757.1"
                     /translation="MNRIPRRNCLGAPAGLAMLLLALFAAGARADDPPAAAFEAANKL
                     YEEGRFAEAAAAYQKLAPPGRTSAALCFNLGNACFKAGQLGRALAAYRQAELLTPRDP
                     DLRANLQFARSQVHPPTLTPGRLQRWLGRLTLNEWTLLAAGAVWLWLLLLAFLQWRPA
                     LRPALRHYVRALALLTALLCAGAAFALRAARFTPTAIVIEPEAVVHYGPLAESPAAFS
                     VHDGAELRVLDQKDDWLQVSAGPRRTGWLRRGQILLPGR"
     gene            25671..26402
                     /gene="infC"
                     /locus_tag="PKI07_06585"
     CDS             25671..26402
                     /gene="infC"
                     /locus_tag="PKI07_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOC50758.1"
                     /translation="MSRPFSSRASASRSFVRVNGKIRAREIRVIDGDGNQLGIMSLGD
                     ALTLARSQGVDLVEVAPNAAPPVCRLVDYGKFRYEQAKRDKESRKHQHATKVKEVQLS
                     PKIDPHDLSVKLQRAIEFLCEGMKVKIALRFRGREMAHTEIGFEVVQRFLASIAAYGQ
                     PDFEPKLVGRGLHVMISPLPRNKRPKAPPPPEATPLAPPPPARAVPAPLAPPAPTSPA
                     ADSRGGESRGGGFVNNPFSGLDLDS"
     gene            26424..27731
                     /locus_tag="PKI07_06590"
     CDS             26424..27731
                     /locus_tag="PKI07_06590"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOC50759.1"
                     /translation="MRHTSRQTSNTSNVLSVIMGGGQGTRLFPLTKDRAKPAVPLAGK
                     YRLVDIPISNCINSGLRRVYLLTQFNSASLHRHISQSYKFDHFTGGFVEILAAEQTYA
                     DNSWYQGTADAVRKNMVHFLNHEWDYLLILSGDQLYRMDFRHIISQHAETDADITVAT
                     IPVARAAAQSLGIMQVDAQRRITRFVEKPKDPAVLDTLKLPPDWLDPLGLPAGGETFL
                     ASMGIYVFNRGIIRGLLESKLTDFGKHVIPDAIQNLRVFSYVFQGYWEDIGTIRAFFE
                     ANLDVTSELPRFNFFDMAAPIFSRPRFLPASKINGAQIDHAVISDGCILNRAIISNSI
                     IGLRCSVGVGTVLNRVILLGSDYYESLESIRAHEQIGRPRIGIGTNCRIQNTIVDKNA
                     RIGDNVVISPDGKPEKVDHANYYIRDGIVIIPKNGVIPHGTVI"
BASE COUNT         4586 a         9439 c         9227 g         4901 t
ORIGIN      
        1 accttgagtg gaaaccatga cgaccaattt gctgaacttg acggacacaa ctccggcctc
       61 ccctttccgc ggccggcccg gcgctcgacc gcccaagccc acccattctt cccttgccag
      121 ccataactcc ccctccgatg gcagggacgc gagaaatgtg gatacccccc ggaaaataag
      181 gcccttgcgc cgcccgcacc ctgaatttac cctgcctata ggccgtgtac aaggggtgta
      241 caagggatgc tcaccgggaa tccatcggat tttagctggt acacccgatg ggcatccctt
      301 gtacacccct tgtacacggc cagggggcag ggtgaatcca cggtatctgg gtaaaggatg
      361 gggatacttg aaaaggccga aacgagggag gaaaacggga ggatgattgc taagctcctt
      421 aaaagcagct agttataaat ctgaggcggg cgggaggggg ttttttaagg gcggacgggt
      481 gtccggatgg cttttggctt cgccaatgta ttatcggaaa cgttaacggt atgcaatcca
      541 gccgtgtgtc cgcgctctac tggggataaa atgggtctgg caggtcagcg gagagcctca
      601 acgaatcggt tggttggtga actcaatggg cagatcgctg acttctgaat gttttgcgcc
      661 cgcaaagcca tggtcttatg cgttccgtgg gattgaatgg agggtgaagc cggaatcgtt
      721 ttcgttcgga tttcgagggc aatggtcgcc ctgcggtttt cccgggggag ggtcgtgcgc
      781 ggggagtgcc aagtttcagg ctggccagcc caaatgctgg acactggcgg gattaggccg
      841 ttaaaccggc gtgtctttaa ccgggcggcc tgctggcggc gcaggggtga ttgtattctg
      901 caagtctttg aaaatgccgg ggatggggac gttcggggcg gcggggattt gagccacggg
      961 cgcggtggcg tcgccgggcg gggcgttggg ggcggctgcg gataggattg gcaacagtct
     1021 tgcgtagaac gtacttgata tgctgtttgc agcaagcagt ttgcaccgcg tgtgccgcgt
     1081 agtgtccgtg atgattgggt gcgccggatc tctggctttt tcgtcttttt cgacgtttgc
     1141 cgccagcagc gtgaccgtgg cctggagtcc gaatgcgaac ccggagatcg ccggctaccg
     1201 ggtgtattac gggggggcca gccgcgttta tacgaaccag gtggatgcgg gccgggctaa
     1261 cagcgcggtc attaacggtt tgaccgcggg cgcgacgtat tacctggccg ccacggctta
     1321 tgatgtggcg ggtttggaaa gcgattattc ggtggaagcg gtcttcacga acctggccgt
     1381 tctgccgcct accatcgttt taagcgcgcc ggccaatggc acggtcttta ccgcgccggc
     1441 gaccctccat ctcgccgccg acgttgccgc caacggccac agcatcgtca aggtgcagtt
     1501 ttataacggg tcggtgctgt tgggcgagga tgccaccccg ccgtacgagt ttgcctgggg
     1561 caatgtgagc gagggcatct acgacgtgaa agcgcgcgcg atttatgacc tcatcggcct
     1621 ggtgacctcc ccgccggtgc gggcgaatgt gtacacgccg cccaagccgc cgaacacccc
     1681 gccgatcctt tcggcgttgg cggaccaaac cctgattgcc ggctccgggc cgctggtggt
     1741 gaacttccag gtcggcgatg ccgagagcgc gcccggcaaa ctggcgctgg ccgccgagac
     1801 cagcgacgcg aagctgctgc cggtggagaa cattgtcttt gacggcagcg gcaacaaccg
     1861 cgttgccacc ctgaggcccg tggcggggcg gaccggggcg gtcgaagtca cgttcatggt
     1921 tcatgatggg gaggcggcca cgagcgcgag tttttggctg accgtgatcg agaagaagcc
     1981 ggagccggcc ggcattgccc tggtatcgcc ggtgggcgcc gcgaatatga actcgaccca
     2041 gcgctacgtc tggcggccgg acccggcggc gacgcgctat gaactggagg tgacccggga
     2101 tggccggtta ttctgcgata actggtacga cgccatcaac ctgccggcgg acccggagac
     2161 cgccaatgta ggcgtcgagg tggccgggca cagcaacggc aattaccagt ggcgggtgcg
     2221 cggctggagc gcggcggggc tgggcccctg gtccagtgcc gggacgtttg ccgtcgccac
     2281 gccgcggccg gtggttttgc tcacgccggc cgatcaaacc agcctccagg aacggcgtcc
     2341 gcagttttcg tgggagcagc ccgtgccgcc cgccgagtgg ttccacctgt gcatcaaccg
     2401 caacgggagc cggtgttatg atctttggct cgagggaacg acccagtgga cgccgtcggt
     2461 ggatttgccg ggcggcgcct acacctgggc ggtgcaggcc tggtctcccg ccggggtcgg
     2521 gccctggtcg aagatggctt cctttaccgc gcagccggcc ctgccggggc agatcgcgct
     2581 tttgaccccc aagagcagcg cgctccgcgg gcccacgctg cgctatcatt ggagccagga
     2641 ttcggcggcg gtgtcgtatg agctgtacat cacccgggat ggcgcggtct tttatgacca
     2701 gtggctgtcg gtgaccaatt tatcggccga cccgggcaag ggcagcctga gcgtggaggt
     2761 gcccggccat gcggcggggt cgtattattg gtatgtccgg ggctggaatc ccgacgggtt
     2821 tggcccctgg tcccgcccgg ggagctttag cgtggacggc gcgtccaagc cgctgaccct
     2881 gctgacgccc accaacaacg ccgtgctgca ggaacggcgg ccggagtttt cctgggtggc
     2941 cgcctcgccc ccggcggccg cctaccatct ttacctgagc cgcagcggca ccaactacta
     3001 cgatgtccgg gtggagggaa agaccaactg ggtgccctcc ttcaacctga cgggcgggtc
     3061 ctacacctgg atggtgcagg cctgggatgc ctccggcagc ggcgccagct cggagacggc
     3121 gagcttcacc agcccctaca tcgtgccggc cgccattgtg gcgctcacgc cgcggggcac
     3181 tgccggcgac agcgcgacgc agcgctacca gtggcaggtg gatgccgcgg ccacgcggta
     3241 tcagttgtat atcaaccgcg acggcagcgt atttcatgac cagtggtatg acgccgccca
     3301 ttcggtgctg gatccggcga cgggcaagtt cgccgtggac gtgccgggac acagcccggg
     3361 gacgtatcaa tggtgggttc ggggctgggg tccggagggg atggggacct ggtctgacgc
     3421 cgtgactttc cgccaataaa ccgcatcggt tccgccgccc ggcgccgctt cgtcccgcgg
     3481 cgccggcgtt tttgcagtac cccaaaaccc cggcggccac cggcccgcgg gcgggaggga
     3541 ttcccgtctc cgcgagggcg gccgccgggg cgtgagggcc ttcggcggcg gttttaccgg
     3601 cagccgtgcc ggtgccagcc gtagccgaag cttacgcgga cggcgggagg cgcgacgcac
     3661 acgggaggcc gatagaccac gaccgccggc gcgggcgcat agacgaccgg cgccgggggc
     3721 ggacaaacca ccaccggcgg cggcggcggc ggcggataat aaaccggcgc gggcgccaca
     3781 tagcagggcg gcggcgaagc cagggcgtgg ctgacaatca gtcccgccgt cacgccgccg
     3841 atcaccgccg cggccgtgcc ccagccgccg ccgccacaat ggcggccgcc cgccagcgcc
     3901 gtctgcgcac tcattccccc cagggccagg gttgtcatga tcaggattac ttttttcatt
     3961 ggttaccttt ccgttcaggt ttcaccgagt acccataaga cgggcggatt tgatttttat
     4021 tcaacccggg gcaaagctag gatgcacgac aagctatgaa gaaacgttct ccttatcggc
     4081 cgccggtgag ccgcgagcgg ctcccgcgct cgttcaagga atttacgccg gcccggcatt
     4141 ttaatccgcg caccttcttt cgcgagctgg tctggaaggc gctgctgacc ccgcggacgg
     4201 ggcagacccg gcagccggtc tttcccaagc tgaaccgcgg gcaggtcgcc atcacctgga
     4261 tcgggcacgc ctcctttctc ctgcagttca ccaacctgaa cgtgctgatt gaccccaact
     4321 tcgccaactg gctgttcctg ctcaagcgcg tcaaacggtc cggcctcagc ctcgaacacc
     4381 tgccgcccat tgacctcgtg ctgctgaccc acgcgcattt cgatcacttc cacaaaccca
     4441 cgctccgccg gctgccgcat ccgaagatcg gcgtcatgcc gtggggggtt ggcgacctgg
     4501 cgcagaacct cggcttcggg cgcgtggtgg agctggagtg gtgggaaact ttttgccatc
     4561 gcgattggaa ggtgacgctg accccctcca aacactgggg cgcgcgcgtg ctgcgcgacc
     4621 agcggcgcgg gtatggcggg tttgtgctgg agcaccaggg gcggcgcatc tatcacgcgg
     4681 gggacagcgc ctacttcgag ggcttcaaag aaatcaaaaa gagctgccgg ccggaaatcg
     4741 ccctgctgcc cattggcgcg tatcatcccg agtcgtttcg caacctgcat atgggccccg
     4801 atgaagccat taaggccttt cgggatctgc gcgcgcgctg gctcatcccg atgcactacg
     4861 gcacgttccg gctgtcgttt gaagatatgg acgcgccccc ccgctggctg cagcagttgg
     4921 ccgtggagca ggggcttacg cataaggtgc gcatgctcgt cgagggcgtc ccccaggtct
     4981 tctaagccgc gggcgttcat cccccgaagg aaccagcccc cgcgcgccgc gcgagggggc
     5041 gcggggatgc cgttcttccg cggagcggtt tttcccgtcc cggaatcagt ggcggaaaaa
     5101 agtgcattgt ggggcttgac gccccaatac ttggggtgtt aaaaatgggt gccacaatta
     5161 gctgtggttg cgcctgtatg cgatgcccta aatgcggttg ccaggatgat aaagtgattg
     5221 actcccgcgc ctcgcgggag ggtgccacga ttcgccgccg gcgggagtgc gttgcgtgcg
     5281 gttttcgatt tacgacctac gaggaaatcg agcaggccgg gttgatggtg atcaagcacg
     5341 acaagcggcg ggaggagttt tccaaggaga aattgctgtc cggcctcaag aaagcctgcc
     5401 agaagcgccc catcagcccg aaggtgatcg aagacctggt cgaaaagatt gtcaatgaga
     5461 tcaccgacaa atatgagcgc gaagtcccgt ccgaggtcat cgggaaactg gtcatggccg
     5521 ggctgcgcac gattgatgat gtggcgtatg tgcggtttgc cagcgtctat cgccgcttcc
     5581 aggaggcgac ggattttgtg caggaagtga agaagctgga ggcccggccg tgattgcgcc
     5641 cggctcggat tggctgtttt tggagctgga caacggcgag cgcgttccct gctccgccga
     5701 catggtggac gtggagctgg cgtgcagcgc ggacgggttg ttcgacgccg agttcatcca
     5761 ccaggtcacc cgggcggttt ttcattattt caagcacgag ctgggacgcc aaagcgtcgc
     5821 cgccgccgag ttcgccgggg cgatggagaa agtgctgcgg ggctttacga ccgcggccgg
     5881 ggccgccgcg gcgccggtgg ccgcggcgcc agagttgggg gcggatttgt gccggctggc
     5941 ccgcgaatcc gggccggggc gggagttgat tttctttccc cgcctgcggg cggagttgcg
     6001 gcggtgcctg ggcgcggggc cgcggtggct gcggtttagc gggctgcggg cgtgcgtcaa
     6061 gcatttggcg ggcgcgcggc gctggagccg gcgctgccag gcgctggagg gggagattgt
     6121 ggcctacctg cgcgaatgtc tccgcgccga gccggagccg atggagctgg tgttgcgggt
     6181 cgagtaagcc gggcgggcct gcatgagcca gacgctgttc gagaagatca tcgcgcgcga
     6241 gcttcccggc acgatcctgt atgaggacga ccaggtggtg gcgtttcagg atattcaccc
     6301 gcaggccccg gtgcatgtcc tgatcgttcc ccggaaaccg attccccgga tcgcggcggc
     6361 cacgcctgaa gatcagcccg tgctggggtg gctgctcttg aaagccgccg aggtggccgg
     6421 ccgcctgggg ctggcccggt ccgggtttcg gctggtgttc aacaacggcc ccgacgccgg
     6481 cgaggcggtc ccgcacctgc actgccatct cctgggcggc cgcccgctgg gctggcctcc
     6541 gggctaggcg cgcgcggcgg gcgcgcggcg cgggccgtca ccgcccgcgc gggcggttgc
     6601 ggagcaccgc gccgaggcgc gcgatgccct ggcgcacgct gtcctcggtg gcgccgccga
     6661 agctgagccg catttcgtga ttgggtttgc ggcgggaggg gtcgtcggcg tagcaaagtt
     6721 cgcccggcac gtagagcacg ttgttcttca gggcggcgcg gaacaccgcg gagttcatgc
     6781 ccgtggcgcg gcggccgggc aactgcgccc agaaatacag tccgcccgcc ggttcctgcc
     6841 attgcacctc ggcggggaaa tgccgttcga tggcttcgcg catcaggcgc gccttgtagg
     6901 cgtagcgctg gcgcaggccg tccaggtgct tcaggtaaag gccggaggac agggtgcggg
     6961 cgagcagttg ctgcagcagg ttggcggagc cgaagtcgtg gttgcccttg atgcggagca
     7021 cggcgctcag gagcggctcg ggcaggaggc caaagcccag gcgggcgccg gacgagaacg
     7081 gcttgctgta cgtgccggtg tagatgacgc ggtcgcgggc gccgttgagg gtgagcgcgg
     7141 acttgatgtc ctcgccgccg aaccgcagct cgcggtaggc cgcgtcttcg agcaggtaga
     7201 gggggtggcc ggcggcccgc tcgaagcgcc gcagcaactg gagcgccgcc gcttttttcg
     7261 cgtagcgggt ggtgcgcccg gtcgggttct ggtagtagct gacgaggtac aggagcttca
     7321 gccgccgcag ttcgccgcgg cgttgcaggt cgcgcagaac gccctccagg tggtccaggt
     7381 ccaccccgtc cgcttccatg cggatgccgc gcgcctggat cccgtggctt tgcaggatgc
     7441 tcaggtaaac gaagtagctg gggtcttcca ccagcaccag gtcgccggcg tcgcacagcg
     7501 cctcggtgag catatagagg agctgctgcg agccgttggt gatgatcatc cgctccgggg
     7561 aataggcggg gcgcgaggcg ggctgttcgt cctgccggca cagatgctgg ctggtgagct
     7621 gccgcagggc cgggtcgccc gccgtggtgc cgtattgcag ggcggtctgg ccggtttggg
     7681 gcgagctgag gatttcgcgc atcagccggc gcactttctc ccgcggcagg gatttgttgt
     7741 cggtgaaccc ggcggccagg gaaaccagtt gcggccggcc cagcgccatg gccagcagcc
     7801 aggagatggg cgggtcttcg gtgcggcggc ccaaagcgga taagtgatgc gctttcatgc
     7861 ggtgtttaac atagcatagt tggccggttt tggccccggg aggcaagcga tcttttgcgg
     7921 cgctccggcg cgcccgggtt cccggtcagc gcgcgggcgc gggggcttcg aactggcgca
     7981 ggaagttttc caggcccgcg aggtattgcg cccggttggc cagcgggctg ttgtggtcgc
     8041 cgctgatttc ctggaagagt ttgggctcat tggcggcggc aaaattggtt tgggcgtggt
     8101 ggaacccgat caagtcatcg tgggggctgt gcatgatcag caccggcagg cgcaggccgg
     8161 gcagtttggc gcgcgtgtca tagcgaatgg tgttgatcca gcgcgccggc agccagggat
     8221 acagctcggc gccgatggcc gggatgctgg tgaacgtgct ttgcagcacc agtccgccca
     8281 ccggctcgcg cacggccagc tccgccgcca cgccgccgcc cagcgagtag ccgtaggcaa
     8341 tgatgcggga gcgcgcgaag ccccgctgcg ccagccattc gcaggcggcc tgggcgtccc
     8401 ggtacgtccc ctcttcggag ggccggccct ggctgcggcc gtagccgcga tagtcgaacg
     8461 cgaacacccc cacgccggcc gtcagcagcg cttcgtaggc ttcgagccgg ttgccgatgt
     8521 tgcccgcgtt gccgtggcag tataacacga ccaggtgggc gcgctcggac tgggggtccg
     8581 ccgggtagaa ccagccgttc agctccagcc cgtcgcgcgt gcgaaaacgg acatcctcga
     8641 agggccggtg cagttcggcg ccggtggcgg tcaggacccg gtcggggtgg taaaccaggt
     8701 tgcgttcgag ccagcgaaac ataaggatga ttagcaggcc ggcaaccaac aggccaatca
     8761 ccgtctttgc caggcggggt aatcgtttca tgccatccgc aagataccac ccggccggcc
     8821 cggctgacaa cccttgcccg cggcggcccg ggcttgccgg cgggggcccg ggccgcgcgg
     8881 cgcggcgggc ggcgggcggt ttgcgcgttg gccggcttta cattccggcc tgaatatccg
     8941 aaattaaccg gcacagaaat atgtcaacta ccgtccaaac ggccttgccc ggcggcgagt
     9001 ggtcgccgtc ctcgtggcag agccggcccg ccgcccagca gccggactac ccggatccgg
     9061 cggccctgcg ccgcgtgctg gcccagttga gccgtttgcc gcccctggtc accagttggg
     9121 aaatcgaaaa cctcaaacgc cagttggccg acgccacccg cggcgagcgt ttcctgcttc
     9181 agggcggcga ttgctcggaa agtttcgccg actgcgagtc cggcgccatc gccagcaaac
     9241 tcaagattct cctccagatg agccttgtgc tcgtccaggg cagcaaaaag cgcatcatcc
     9301 ggctgggccg ctttgccggc caatacgcca aaccccgctc ggtcaacacc gaaacccacg
     9361 acggcatcag cctgcccagc taccgcggcg acatgatcaa ccacgccggg ttcaccgcgg
     9421 aagaccggcg gcccgacccc gagctgctgc tccgcgccta cgaacgctcc gccctgacca
     9481 ttaacttcat tcgctcgctg atcgagggcg ggtttgccga cctgcgccat cccgaatact
     9541 gggacctggg ctttgtggcc ggttccgcgc acgccgccga atacacgcgc atggtcgaga
     9601 ccattggcga atcgctgcgc ttcatggaaa cgctcaccgg gggcgtgctg gcggacatca
     9661 accgcgtgga ttttttcacc agccacgagg gcctgcacct cgcgtacgaa caggcccaga
     9721 cccggcaggt gccgcatcgc accggctggt acaacctgtc cacgcatttt ccctggattg
     9781 gcgaccggac gcgcgcgctg gagggcgcgc atatcgagta tttccgcggc atcgccaacc
     9841 ccatcggcct caaaatcggc cccagcatta cgcccgagca gctcgtcgcc ctggccgggg
     9901 tgctcaaccc gcaaaacgag ccgggccgcc ttaccttgat ccaccggttc ggagtggacc
     9961 gcatcgagcg ttgcctgccg ccgctggtga cggccatcaa ccgcgccggc cgctgcgtgc
    10021 tctggtgttg cgaccccatg cacggcaaca ccgagacgac ccgaagcggc atcaagaccc
    10081 gccgcttcga gaagatcctg caagagctgg aagccgcctg ccgcatcctc caggattgcg
    10141 gcgcgcacct gggcggagtg catttcgagc tgaccgggga caacgtgacg gaatgtctcg
    10201 gcggcgccag cggcgtgacc gaggccgacc tgacccgcga ctaccgcacg gccctggacc
    10261 cgcgcctcaa ttacgagcaa tccatggaaa tggccctgct cctcgcgcgc ctgttgtcgc
    10321 cgcggcggaa gccctgactc cgcccggcgt ccgcccggcc cacccccggc atttcccccg
    10381 ctcatcagcc gcgcgctccc tgcgggcatg gctttgggat ggccaggaat gggcttggac
    10441 tggcgacggt tgcgcaatga cggtaatgcc ggtgatccga aaggcaaaat gacggcccaa
    10501 tcggatattt ggaggcgtat cctttaattg gatggcccag ggtggagagg gggtagtggc
    10561 ggtttcattg ggaagcgcac cgttaaaaaa aggggcggcg gcggcccggt ttcggaggca
    10621 ggttgatgct gacgcggttg gaggcggcgg cggcaccggg ttgccacggg cgcccaaagt
    10681 ggcagaaatc ctgatttgac attacggcgg gaatgatgta agcttattcc cagttgggag
    10741 cagagtagat taagcacgcg ataaacatta tcggcataag ggagtcaact ccgttagaag
    10801 gttaattgcg acgccgctaa agtcgaatat tgttccgcac gccaaccccc ttcccgccat
    10861 gaaaaagcat tgttatcttt ggttgattct aatcattttg acaggcctgc acgccgttcg
    10921 cggccaaccc gtcaccttcc cggatgccgg gctcgaagcg gtcgtccgtg aggcgattcc
    10981 caaaccgtcc ggcacgatca ccgtcgcgga tatgttaacg ctgaccaatc tggtggtggc
    11041 gagtggccgg ggcattgagg accttgccgg gctggagacg gcgcgcaatc tttggcggtt
    11101 ggagttggga tggaacccga ttacgagcca caccgccctg gccagcctga ccaacctgct
    11161 cctgctgtcc attagcgcca atgacaaccc ggacatcagc tttgtggccg cgcttaaaaa
    11221 gctgcgggag ctgaacctgc ccaacaacgg gatcaaggac atctctccgc tggccggctt
    11281 gaccgatttg caacggcttt acctgaatta taatgcggtc accaaccccg ccgcgctttc
    11341 cgccctgacc aaccttgttg aactggttct cgactttaat aacgtgagca atctttcctt
    11401 tgttgtcccg ctccgaaagc tggagcggct cagcctgaat aacaaccagg tttcggacct
    11461 ttccccgctg gccagccaga cgaacctgct ttatctcaat ctcaactgga atggcgtgac
    11521 caatcccgcc gtgctgacga cgctcacctg tttgcgggaa ttgacgttgt gcgggaataa
    11581 tctgatccag gtgccgttcc tgtccgggct gaccaacctt accgggctgg atctggccta
    11641 caccagtctg gcggacatgt cgccccttac gaacctggcg cggttggagt ggttgaatgt
    11701 gggagagaat ggcctggccc aacttccgga tctgtcggtg ctctccaacc tgcggacctt
    11761 tatgatggca ggcaaccaga tcaccgatct gacgccggtc acccgcctgc ccggggtctg
    11821 ggacctgcac ttgcaacgaa atcccttcga ggatttgtcc ccgctcaccc actgcccgca
    11881 cctccgggtt ctgttatttt acgacaacca taccatcacc aacctctccg tgcttggcgc
    11941 ggtgaccaac ctggagacgc tctacctggg ccacatgtat ttttccgatc tccagcagat
    12001 tgatttcctt gccggcctgg ccgggctgag gggcctggat ctctccgaaa actatctcac
    12061 cgacctctcg cccctcacca actaccccag cctcagttgg ctgggactcg cagccaaccg
    12121 cctgcagcac atcggtccgc tgcgcaactt gccaacgctc gactacgtgg acttgagcgc
    12181 gaattatctc gacctcaacc ccgccagcgc cgcgtggagc gttattcccg agctccagga
    12241 tcacggcgtt tatgtggaat ataacgcgca gcaggtggcg ccggcttccc cgcgcatcct
    12301 gcggcagccc gccaaccgct ccgccttcgc gggcgacccc gtcaccttca ccgtcctgct
    12361 ggaaaacaac ggcggctggc cggaattccg ctggcagaag gacggcgtgg acctggagaa
    12421 cgacgcgcgc tatgacggcg tggatagtga ccaactaatc ctgtacgcgg tcactgccgc
    12481 cgaggcgggc cattatcgcg tgcgcatctg gcgtgactgg gttgagactt acagtgcggc
    12541 cgccgagttg cgggtcgtca ccaacgtggc cttcgccgac ccgaacctgg agcaggcggt
    12601 gcgcgatgaa ctggggattc cggtgcagcc gctgaccccc gcggacttgg ccgggttatc
    12661 cgtcctcagc gccggctatc gcggcatcac caatctggcc ggcctggaag ccgccgccta
    12721 cctgacctcc ctcgaccttt ccggcaaccc gtaccttgat gactttgcgg cgctgacgta
    12781 cctgtcctcc ctgaatgagc tggcgataaa cgacgccggg cgggacaccc tcgactgggt
    12841 cgcgaatctc cccacgctga acctcctttg ggccggcgag aattttctgg aagacctgtc
    12901 gccgttgcac gcgctgaccg acctgatatc gctcgacgtc gccggcaacc agctcacgaa
    12961 tattgacccg ctgctggatt tgccggcgct gggcagtgtc aacctgagct tgaaccgcct
    13021 cgataccgat gccaactccg ccgcgtggaa cgtgatcacc aatctgcaag cgcgcggcgt
    13081 cacggtggac tatgatcccc aatacgacgc gccggcccct ccgctcatca ccagccagcc
    13141 cgcaagcgtg gtcgcgtacc tgggggataa cgtcagcttc catgtggagg ccagcggcag
    13201 tccaaactat tggtggttaa agaacggcgc gaacctctgc gacaccgaac gcatcggtgg
    13261 cacccgcgac gatacgctct accttgacaa cgtgcaggcc agcgacaccg ccagctatcg
    13321 cgtccgcatc tgggatgaat acggcgtcac caacagccgc accgtcacgc tgcgcgtcat
    13381 cacccaggtg gcgttcgcgg atccgcgcct ggaacaggtg gtgcgcgacc atctgggcat
    13441 ccccacccgg ccgctggtgc ccgcggatgt cgcgcccctg acctggctga atgcctggaa
    13501 ccgcggcatc accaaccttt cgggcatcga gtccctcgcc aacctggaca tgctcaacct
    13561 ggacagcaat cccggcctca cggacctcgc gccgctgctc cagttgcccc ggctgacccg
    13621 tctcaacttg aacaactgcg gcgtccgcga tctcgccttt gtggccgccc tgccgccgtt
    13681 gacggaactg catgtctggg gcggcaggtt caccgacctt gctcccttgc tgagccaaac
    13741 cgggttgcag tggctcaatc tcggcaacaa ccccggcctg gccgacttca ccgtcctcga
    13801 tcacctgacc gagattcagt acctctggct tgattccgtc ggcctcacca acctcgcctt
    13861 cgtgggctat atgccccaat tggtcgaact caacctttgg aacaacgcgg tgtcggacct
    13921 cacaccgctg gccgccgttc cacaactcgt gcgcctgaac gtgggcgcca accagctcac
    13981 tgatttgagc tttgtcggca acctccctgg cctcgagcgc ctggaagtca gcgagaatcc
    14041 catctccgat ctcacgccgc ttatcggctt gacccacctc acccagctcg gcatcggcgc
    14101 gacgctggcc accaacctcg cgccggtcgc caccctcacc aacctgaccg aacttagcgt
    14161 tcggggactg gggctggccc ataacctgag cttcctggct cccctgaccc gcctgcagtg
    14221 gctgggcctc gaagataacg gcatcagcgc gctgccggcg tacccaagcc tggatcggct
    14281 gatcggcctg aatctctccg gcaatccgct ggcggatctg gccagcatcg tcgcccggac
    14341 caacctgagc cacctttacc tcaacaacac cgggctgagc gacctcagtg ccctggccgg
    14401 ccataccaac ctcaataacc tgggcctggc cgataaccaa ctgaccgacc tttcgccgct
    14461 ggccacgctg ccgcacctgc gctgggtcag cctctggaac aaccaggtgc aaaatctcgc
    14521 ggccttcgcg ggcctgacca acctggacta tgtggacctg cgctacaatt ggctgaatac
    14581 caactccggt tcggcggcca tgaccatcat tggccttttg cagggccggg gcgctttcgt
    14641 ggagtatgag ccgcaaaacc tgagccccgg cgaggtggtg ctccgcgcgc cggcctggct
    14701 gggcgggaat caattccggt tcacgattga cggccctccc ggcgccctgc tggaaatctg
    14761 gcggtcaacc gattggaatc attgggattc gctgggtttc gtgaccaata ataccggcac
    14821 gaccgtcttt acggattcag ccgcccccgc cagccgcgcg ttctaccgcg cgcaactgcc
    14881 gtagtcgggc gcccgggatt tccgccccgg ccggaaacgc gggcgcgggc gcgggccgga
    14941 tgttagggtt gtgccgcggc gggatttggg gttaacttcc gccgcgatgt gcgcgggcgc
    15001 gatggcgttg ggatttgcgg ggtgtcttcc ggcggcgagc cgggcgcgcg cccggcgtgc
    15061 ggcgtcgtcg gaggagccgt ttccccgaag gccatgaagg agggattgct ttccccacgc
    15121 cggcggcgct ggattattct cgtgcttatt tttggcggga ttgtgctcaa ttactttgac
    15181 cgccagatca ttgcgatcct caagcccacg attaaagcgc agttccacct ggacgatgcc
    15241 gggtatgcgc tgctgaccaa tatattcacc gtctgctacg cgggcatgta tccgctggcc
    15301 ggctggctgg tggaccggtg gggggcgggg agcatgatgc tggccggcct gctggcgtgg
    15361 tccagcgcgt gcctggggac ggggctgacg cggacctttg ggcaactggc cttttgccgc
    15421 gggatgctgg ggctggcgga gccggttgcg tttcccgccc aactgcgcgc cgtgaccctc
    15481 tggttccccg gcagcttgcg ggccaccgcc aacagcctgt gtgtggcggg cagttccatc
    15541 ggggcgattg ccgcgccgcc gctggtggcc tggctggcct tgaagtggag ctggcaggcg
    15601 gcctttatta tcccgggcgc catcgggctg gggctggcgg ggttgtggcg gctgctctac
    15661 cgcgaccctc ccgccgaaat gatgaacccg gtcgccgacg cggcggagcc cggcccggcg
    15721 gagaagttcg cctggccgca actgtggcgc acgcgcagcc tgtggggcat cgtgctgatc
    15781 cgctttatca gcgacccggt ctggtatttc tgcctgttct ggctgccggg ctacctgcag
    15841 gagcagtccg gcctgacgct ggcgcagacc ggccaggtgg gctggattcc ctttctggcg
    15901 gggggcctcg gcggcgtggg cagcgcggcc tggtcggact ggatggtaaa gcgcggcctg
    15961 ccgccggtgc gcgcgcgcaa gctcatgctc acgagcatgg ccgccctggg gccggtctgc
    16021 gcgctcacgc cgcatctgcc caatgccgcc gcgaccctcg ccattttcag catcgccagc
    16081 gccgtctgcc tgagctggct gtattcactc agcgtggtga tcgcggaagc cttccccacc
    16141 gacaacatcg gcagcgtgct gggcatcgcc gccggcttcg gcgccgccgg ggccatgctg
    16201 ttcaattatt ttgtcgggca ggtcatgggc acgctcggcg cggccaatat gttcatcatc
    16261 atggccctgt tgcacccgct ggcggcgctg gtgttgtgga cgatggtgcg gcgcgagcag
    16321 ccgaagcgcc gcgccacccc gggcccgggc cgatgaaccc tggtgcccat ccctgcgcgg
    16381 agcccggccc cgggttgccg gcggcccgcc gccccacccg cgcttcccgg gcggcaacga
    16441 atcgcgcgcc ggcaggctat acaaccgggg cgttctgtgt tctaatggga gcgcgcgtat
    16501 gaatcgtcga acctttatcc ggaaccttgg gctgggcgcc acgctgccct ggctggggcg
    16561 cgcggccctg gcggcgccgg cccgcgcggc ggtgcacacc ctgctctcct gcaatatccg
    16621 cgtggcgctg cccgaggatg aggccacggg caacggctgg aaagcgcggc gcgagctgtg
    16681 cctggaggtc atacgcgccc aacagcccga cctgatttgc tgccaggaag tcctgcgcga
    16741 gcagatggac gatttgacgc gcggcctgcc cgagtttgcc ggcttcggtt tcgaggggcc
    16801 ggagatggac gcccggaagg aaggctacca gggcattgcc aagaatccca tcttctacgc
    16861 gcgggaacgg tatgagctgg tcaccgcggg cggtttctgg ctttcggaaa cccctcacct
    16921 gcccggctcg ctgtcgtggg aatccgcccg cgcccggcat gtcaactggg tgcgcctgcg
    16981 cgagcgcgcc tcgggccggt cgttccgcgt gctcaatacg cacctcgacc acaaaagcca
    17041 gcccgcccgc gaggcgcaga tgaaaatgat cctggccgag gccgcgctct acgcgccgga
    17101 tttcccgcag cttctggccg gcgatctgaa cgcgggcgcc gccaacgccg tcctccagct
    17161 cgcgcttgac gcgggctgga gcaacacccg caccgccgcg ccggggccgc cggaggaggg
    17221 caacactacg catgaatttc tcggcccgaa atatgtcccc aaaacccccg gcgcccgcaa
    17281 gcggggcccc atagaccata ttctcacgca cggcccggtc accacccggg cctggaagat
    17341 cattcgcgac gggcgcgacg gccgctaccc gagcgaccac tactttctgg ccgttaaatt
    17401 caccctcgac gcttaatccc tcatccccat gcaccgacga caattcctcc gctgcctcgg
    17461 gctggccgcc gcgctgcccg gcatgggccg cgccacgctg gccgccgccg aatccactcc
    17521 cgccgccgaa tccgcccccg cccccgccgc cgccgcgccc ggcggcacgc acaagatcct
    17581 gtcctgcaac gtgcgcggag acctggccgg ggatgcccgg acgggagata gctgggcgga
    17641 gcgccgggag ttttgcgccg acgtgatccg cgcgcagcgg cccgacttga tctgcctgca
    17701 ggaatgccag cagaacaact ggaaatacct gaaactccgg ctgcccgagt tcgactcctt
    17761 cggcctgttc gatcccgggc cggtgccgca tccgatgaac accctcttct tcgcgcgcgc
    17821 ccgctacgaa ctgctttcgg cgggcggttt ctggctttcg gaacagccgc acgtggccgg
    17881 ctcaaagtcc tgggacacgg cctattcgcg gctggtgaac tgggttcacc tgcgcgagcg
    17941 tgccacggac cgggaactgc gcgtatggaa tacccacctg gaccacctcg gcgcggtcgc
    18001 ccgcgaaaag caggcggagt tgatcgtgca ggcggcggcg gtgttcccgg cggcgctgcc
    18061 ccaattgctc acgggtgatt tcaactgccc caccggcagc cccccggcgg agcggattcg
    18121 cgcggcgggc tggacggata cctacgccgc cgtgcacggg ccgaaggatc ccggcttcac
    18181 ctttcacggg tttcaggggc ccgggtttgc cacgcataag ctggcccgga aactctccgg
    18241 gaaaattgac ttcatctggt gccgcgggcc ggtcaagacg ctggctgccg agatcattcg
    18301 ggacggccgc aacggtcatt acccgagcga ccattatttt atttccgccg tcgttgccat
    18361 gtaggcgcgt gcggccgaca actttatgaa caacccagcg cttggatcta ttcaggaacc
    18421 cgcccggaac gtgcccgtga gcgccgcggt ggaagtattg gtggtgggcg gcggccccgc
    18481 cggcatcatg gccgccctgg ccgccgcgga gcagggccgg caagtggcgc tggtcgaggg
    18541 ccgcagcttc gtcggcggca acctcgccct gggcctgccc atcctcggct tcctcagcca
    18601 gaaaggccgg cccatcatca ccggcctgcc ccagcagctc attgaccggc tgcgcgccca
    18661 gggcgccgcc agtgaacacc agccctgccc cctccatgtg agcctgaccc tggtcgagcc
    18721 ggaggcggtg aagtccgtcg cgatggaaat gctgctggaa cgcggcgtcc agctcatgct
    18781 ctaccgccag ttcgccgacg cggtgatgga cggccaccgg ctgcgcggca tcatcaccga
    18841 aagcaaagcc ggccgggagg caatcctggc cccggtcatc attgacgcca ccggcgacgg
    18901 cgatgtggcc ttccgcgccg gcgtgccgtg cgagaaaggc gacgcgcacg gcggcatgca
    18961 gccgcccacg ctcatgttct gcctggccgg cgtggatacc gagaagctac gccgcagcct
    19021 cgccgacgac ccgcaaacct accgcgccga catcatcccc gccgagtact tcgcccggaa
    19081 ccgccagttc atcgtggtgg gcctgcggca gcgcctccag caggcgcagg ccgacggcct
    19141 gacgctgccc accgagcgga ccatcctgat caccggcctg cgcaccgggg aagtctgggt
    19201 gaacatgacc cgggtcaagg gcgtggacgg caccgacccg caaagcctgt ccaccggcga
    19261 gattgccgcc cgccgccaga ttgccgacat ccagcgctac ctcgtccaat acgtgcccgg
    19321 cttcgcgcag gcgagcctcg ccaaggtcgc ccccttcctc ggcctccggg aaacccggcg
    19381 catccggggc cgctatgtgc tcacccgcga ggatatcctc ggatgccgcc gcttcgacga
    19441 cgccgtcgcc gtggccagct acccggttga cctgcaccac cccagcgacg acgactgcac
    19501 gctggaatgg tgcggcgatt gctacgacct gccctaccgc tgcctggtgc cgcagcaggt
    19561 ggaaaacctg ctggtcgccg gccgctgcat ctcgaccacg cacgaagcca tggccgccat
    19621 ccgggtcatg tccacctgca tggcgaccgg ccaggccgtt ggctgcgccg ccggaatggc
    19681 ggtcgcggag cgcatcccgc cttccgccct caacgtctcc cgcctccggg acgaactccg
    19741 ggcgcgcggc gcttacctgc gcgactagcc gccgcccggg gcggccacgc cccggctaga
    19801 cctcgcggat ccagcggcgc tgttcccaca ccgcgcggag cgaggtgagg ttgcgcgggt
    19861 cgcagagatt tctggccgcc gccaggagcc ggccccgctt gaggtggttc cacgcgtcgc
    19921 tgacgttgac ggcgatgaat tgcagcacgc tcgggtcgcg atagcagtca atcatgcagc
    19981 gcgtgcagtt gtcgcggatc agcttcgaac gatcgaaatc gtaaatgcgg cacatggggg
    20041 tttcccagaa atggcagcgg taaaggttca ggtgccagtc caggtagaag tatttgtagc
    20101 cgcccaggca gccgaacttc tccggctcgc cgcgcaaatg ccgctgcatc tcggtgagcg
    20161 attcccgggg attgaccacc gggaaacccg tgcggcgctt catgcgcttg atgcgctcga
    20221 aaacctcgat cagctccgcc ggcgaatagt tgacgagctg cgaaccgctg aaactcaggt
    20281 agctcgaacc caggctgcgc agcgggtaac tgaacgtgca gctccggaac cccagcgcgg
    20341 tcaggaaggc gggcagccgg tcataatcct caatcagccg gctggcgatg atgctggcgg
    20401 tggtctggac gccgagccgg gcgaacacct cgttcgcccg ctgaatcttg cggcacacgt
    20461 ccggcaggcc gcggctctgc tcatgccgcg ccgcgtccgg cgcgtcaatg gacatgatga
    20521 cgctgcataa accgtcggcg gccagctccc gcatattccg gtcggtccag agggagccgt
    20581 tggtgcagat catcgggcgg atgccgcgct cggcggcgta gcgggtcatg gcgcgcacat
    20641 ccggatgcgc cagcggttcg ccgccgacca acaacaggta gccaatgtgg ttccggacgc
    20701 agatgtcaat cacgtcccgc gcctcggcca gggtcacgct gcggcgctgc ttcggatcca
    20761 actggtccac cgcaaacccg caaaactcgc accgggcatt gcaccggttg gtgatggcga
    20821 actgaagata acccgggccg ccgtcgcgca gcacctcgcg caccagttgc cacgggccgg
    20881 tgcgcggccg gggcaccggg cgcagggcgc cggcatccgg ccccgggggg gcgaacggcg
    20941 catcggaacc agtggcgttg gcgggacgca tacggttttc tatccgctca tcttggcggg
    21001 aagcgccgcc gcggagcaat cctttcttct cccttgatgc gccgctccct tttccccggc
    21061 ccggccgcga gcctccgcgg cgcaagacgg ctccggtttc cccggcaagc tgcgtcccgg
    21121 ccggcggggg ccggtgcggg agcgcgggcg gaggaggggc ggtgttccgt tgcgggacgg
    21181 cggcttaacc catacagtaa cgcccgatga aacccaaagg aatggtttat ctggtgggcg
    21241 ccgggccggg cgaggtgggg atgctcacgc tgcgcgcggc ggaagtgctg gcgcgcgccg
    21301 aggtggtggt ggcgggcgag ggggtttccc ccgaggtgct cgcgctggcc ccgccgacgg
    21361 cggagtttat cggcgcgggg agcggaggca gcccggcggc gcagtccccg gaagaggtgg
    21421 gccggctgct ggcggcctcg gcgcgcgcgg gcaagcgggt ggtccggctg atcgggggcg
    21481 acccgtactt attcggcggg ggcggcgagg aggcggaatg gctggccggg gcgcgggttc
    21541 ccttcgaggt ggtgccgggg gtttccgccc tggtggccgg gccgaactgc gccgggttcc
    21601 cgctggcgca tcgccagtgg gcttcgcaaa tcacgctttt ggccgggacc gaagcgccgg
    21661 cggcggttga ctgggcgcag gtggcccggg cgccgggcac ccgggtgatt gcgctggcgc
    21721 cggagcggat cggcccgacg gtggaattgc tggccgcgca ggggatggcg ccggagacgc
    21781 cggtggcggt ggtgggcccg gcgctccgcg ggcggcatca cgtctggcag ggcacgctgg
    21841 ccaccatcgc cggagtggtg gcccgggagg aaatccgccc gcccgcgatg ctggtcatcg
    21901 gcggcgtggt ccaactgcgc gccaggctca actggttcga gcgccggccg ctttttggcc
    21961 agcgcattgc cattacccgc gcgcgcgcgc aggcgggcga gctggcgcgc cagttgcggg
    22021 aactgggggc cgaggtgctc gaggttccca cgctccggtt cgaggcgccc agccgccgcc
    22081 aggacttggt ggacgcgctg ctggagctca acgcgtatga ttggctggtg ttcaccagcc
    22141 ccaacggcgt cacccgcttt tttgaatact tcttccggcg ctttcatgat ctgcgcgacc
    22201 tgggcggggc gcggctggcc gcggtggggc cgggcaccgc cagcaagctc cgggaactgc
    22261 atctgcaagt ggacctggtg cccgacgagg cgctgggcgt ggaggtggcc cgggcgttct
    22321 cccaatttga aagcgtcgag aacctgaaga tttgcctgct gcgcgccgag gcggcgaacc
    22381 gggatttgcc cgaggcgctc acggcgttgg gagccatcgt ggatgacgtg gcctgctacc
    22441 agacggtgcc cgaaaccgaa gacgcgaccg gcgccgcggc cagcctgcgg gagcgcgggg
    22501 cggattgggt ggtgtttacg agcggatcaa cggtgcgaaa cttccatgcc cggttcgatt
    22561 tgcccgccct gcagcgccag ttcccgcagc tcaaggcggc cgtcatcggc cccgagacgg
    22621 cccgggcgct cgcggcgctg ggcgtggcgc ctgcctgcga agcgcgcccc cacaccctcg
    22681 aaggcctggt caaggtgctg ctcgccgcgg cggcggcgaa ataggcggcc agcggggaca
    22741 tgctggccgg ggaggcgccc ggcctcattt ctgtccgaac aaaacgccgt tgacgttgtc
    22801 catatagcgt cccctggcgg ggcgcaatcc gacgcggcca gcggcccggc aagggaaact
    22861 tgcgccgcgc ccgtccgccg ggcaagacta agcggagctg gaaatgcgaa ggttatcatc
    22921 gtggatcaag tcaaattcgc gccgccgaag ccggcaagag cttgggcggg gcgtggcgtt
    22981 gctggcgggg ctggcatggc tggccccgtt gctggcgggc gcggccagct tcacggccac
    23041 gctggaccgc gaaaacgtca ccgtgggtga aagcgccacc ctgacgctcc gcttcgaggg
    23101 gggcaatccc aagtccatcc cgttcccgtc cgacattccg cacctgcaga tatcagacgc
    23161 cggcacttcc cggaatatca gcatcgtcaa cggccagtac tcggccagtt tcttgaaaaa
    23221 catcatcctg acgcccaccc agcccgggga gttcgtgatc ccggcgctca ccgccgaggt
    23281 gggcggccag gtgctcacca ccccgccgct gaagttgacc gccgtcaaag cccccacctc
    23341 ggcggtggat ggcgcggggg agcaactggc gttcttccgc ctttttgtgc cccgcaagga
    23401 ggtttacgtg ggggaaatcc tcgccatcga actccaggtc tgcattcgcg agggcctcgc
    23461 caacggggaa gacatcctgc agagcttcga ccagttcaac ggctgcccgc tgaaggtcga
    23521 gggcgtcagc ctcatcaaga cggcccacgc ccagcgccgc cggggacaaa ttggaaacgc
    23581 cggctatggc atcgcgacgc tcgtcacctc gctttcaccg ctccgctccg ggcccatctc
    23641 catcagctcc atggaggtgc cgctcacgtt gcagattccc ttgccgaacc agcgccgccg
    23701 ggacccgttc gaccccttcg gcatgtttca gcgcctccag gtcgaagagc ggcgcgtgac
    23761 cttgtccgcg gagccggaga cgctcgaagc cctgccgctg ccggccgagg gcgttcccgc
    23821 cgggttcaac ggcgcggtcg gcagctttgc catgaccgtc acggccggcc ccaccaacgt
    23881 ggccgttggc gacccggtca ccgtcaaaat ccaactcgcc ggccgcgggg cgttcgacgc
    23941 gctggctctg ccggagcaga ccgcctggca ggatttcaag acctatccgc ccacggccaa
    24001 ggtggagacg accgatccgc tcgggttgca aggcgtcaag acgtttgagc aggtggttgt
    24061 gccgcagaac ccggagctca aggcgctgcc cgccttggcg ttcagctttt tcgatccgga
    24121 gcaaaaacgc taccgcaccc tgacgcagcc gcccatcccg ctgatcgtcc gccccggagg
    24181 gccggcgccg gtggcggtgg cggcggcctc cgcgcgcaac cccgcggcgg aagccccgcc
    24241 gcccgcgccc gacattgtgc atatcaaacc gcggctcggg gcgatggccc aggtggcggc
    24301 gccgctggtt ttccagccgc ggttcctggc ctggcaagcc ctcccgctgc tggcctggct
    24361 ttcggcgttg ctgtggcggc ggcgcgccga ggcgctggcc gccaatcccc gcctgcgccg
    24421 ccgccggcgg gtggcccagg tcgtccgcca gggcctcgcc gaactgcgcc ggcacgccgc
    24481 gggcaaccgc tccgacgact tctttgcgac gctggcgcgc ctgctgcagg agcagcttgg
    24541 cgagcggctg gacctgccgg cttcggccat cacggaggcg gtcattgacg agcatctccg
    24601 cccgcgcggc gtgccggagg cggtgctggg ctccctgcgc gagctgttcc agacctgcaa
    24661 cttcgcccgc tatgcgccga ttaaatccag ccaggaactg acggccatga tccccaaaat
    24721 cgaggccgcc ctgcgcgatc tccaggagct ggaactgtga accgcatccc gagacgcaac
    24781 tgcctgggcg cgccggcggg gctggccatg ctgctgcttg ccctgttcgc cgcgggcgcc
    24841 cgcgcggacg atccccccgc cgccgctttt gaagccgcca acaagctgta tgaggaaggc
    24901 cgctttgccg aagccgccgc cgcctaccag aaactggcgc cgcccggccg gacttccgcc
    24961 gccctgtgtt tcaacctcgg caacgcctgc ttcaaggccg gccagcttgg ccgggccctg
    25021 gccgcctacc gccaggcgga actgttgacg ccgcgcgatc ccgacctgcg ggccaacctc
    25081 cagttcgcgc gcagccaggt ccatcccccc acgctcacgc ccggccgcct gcagcgctgg
    25141 cttgggcggc tgacgctgaa cgaatggacg ctgctggcgg cgggggcggt ctggctgtgg
    25201 ctgttgctgc tggcgttcct gcaatggcgg cccgccctcc gcccggccct gcggcactac
    25261 gtccgcgccc tggccctgct gacggcgctg ttgtgcgcgg gcgcggcctt cgccctgcgc
    25321 gccgcccgct tcacccccac ggccatcgtc atcgagcccg aggcggtggt gcattatggc
    25381 ccgctggcgg aatcgcccgc cgccttcagc gttcacgacg gcgccgagct gcgcgtcctg
    25441 gatcaaaagg acgactggct ccaggtcagc gccggccccc gccgcaccgg ctggctccgc
    25501 cgcggccaaa tccttctccc cggccgctga gggggcgccc ggggggcctg catttagggg
    25561 ttgccaaccc cgggcgaagc gggtaaatat aggcatcgtt tatagcggtc ggcctgtttg
    25621 tatggttcag acaacaactg catgaatcgg tctcaaccca cgggggtgtc ttgagtcgtc
    25681 cattttcttc ccgcgcttcg gcttcccgtt cgtttgtcag agtcaacggc aaaatccgcg
    25741 ctcgcgaaat tcgcgtgatt gacggcgacg gcaatcaact gggcattatg tccctcgggg
    25801 acgccctcac cctggcccgc tcccagggcg tggacctggt cgaggttgcg cccaacgccg
    25861 ccccgccggt ctgccggctg gtggattatg gcaagttccg gtatgaacag gccaaacgcg
    25921 ataaggaatc ccggaaacac cagcacgcca ccaaggtcaa ggaagtgcag ctcagcccca
    25981 agattgaccc gcacgatttg agcgtcaagc tgcagcgcgc catcgagttt ttgtgcgaag
    26041 gcatgaaggt caagatcgcg ctgcggttcc gcggccggga aatggcgcac accgaaattg
    26101 gctttgaagt cgtgcagcgg ttcctcgcga gcatcgccgc gtatgggcag ccggacttcg
    26161 agcccaagct ggtcgggcgc ggcctgcatg tgatgatttc ccccctgccg cgcaacaagc
    26221 gccccaaggc gccccccccg cccgaggcca cgcccctcgc gccgccgccg cccgcgcgcg
    26281 ccgttccggc gccgttggcc ccgcccgcgc ccacatcccc cgcggccgat tcccggggcg
    26341 gcgaatcccg gggcggcggc tttgtgaaca atccgttttc cggcctcgac cttgactcgt
    26401 agcctgccgg gacgaacctt gccatgcgcc acaccagccg ccaaacctcc aacaccagca
    26461 acgtcctgtc cgtgatcatg ggcggggggc agggcacccg cttgtttccg ctgaccaaag
    26521 atcgcgccaa accggcggtg ccgctggccg gcaaataccg cctcgtggat atccccatct
    26581 ccaactgcat caactccggc ctgcggcgcg tttatttgct cacccagttc aactccgcct
    26641 ccctgcatcg gcacatctcg caatcctata agttcgacca cttcaccggc ggcttcgtgg
    26701 aaatcctcgc cgccgagcag acctacgccg acaactcgtg gtaccagggc accgcggacg
    26761 ccgttcgcaa aaacatggtg catttcctga accacgaatg ggattacctg ctcatcctca
    26821 gcggcgacca gctttaccgg atggacttcc ggcatatcat cagccagcac gccgaaaccg
    26881 acgccgatat cacggtggca accatccccg tcgcccgggc cgcggcccaa tccctcggga
    26941 tcatgcaggt ggacgcccag cgccgcatca cccgcttcgt ggaaaaaccc aaagaccccg
    27001 ccgtgctgga caccctcaaa ctcccgccgg actggctcga cccgctgggc ctcccggccg
    27061 gcggcgagac gttcctcgcg tccatgggca tttatgtgtt caaccgcggc atcattcgcg
    27121 gcctgctgga gagcaaactg accgacttcg gcaaacacgt catccccgac gccatccaaa
    27181 acctgcgcgt tttctcttat gtcttccagg ggtattggga ggacattggc accatccgcg
    27241 cctttttcga agccaacctc gatgtcacca gcgaactgcc gcgctttaac ttcttcgata
    27301 tggccgcgcc catcttcagc cgcccgcgct tcctgcccgc ctcgaagatc aacggcgccc
    27361 agattgacca cgcggtgatt tccgacggct gcatcctcaa tcgcgccatc atctccaaca
    27421 gcatcatcgg tctgcgctgc agcgtcggcg tcggcaccgt cctgaaccgg gtgatcctcc
    27481 tgggcagcga ttactacgaa tccctcgaat ccatccgcgc gcacgagcaa atcggccgcc
    27541 cccgcattgg catcggcacc aattgccgca tccagaacac catcgtggat aaaaatgccc
    27601 gcatcggcga caacgtggtg atttctcccg acggcaagcc ggaaaaggtg gaccacgcca
    27661 attactacat ccgcgacggg attgtcatca tccccaaaaa cggcgtcatc ccgcacggaa
    27721 cggtgatttg accccggccc cgaacccccc tcgctaaccg cgcccggcca atgccggctt
    27781 ccccgccggc cgcggcggat tttccggcgc gccgccctcg cggttggatt cgccagccaa
    27841 gcagttcgtt gaggccgcct gcgggtctgt cggatatgtc ccggcctcga tggagcgaga
    27901 gcgcacggct aaagcaccca acgtcaacgt ttcaggtaag acattcgcga agccaaaagc
    27961 cctccgaacg cccgcccgcc cgtgaaaaat cccctcccac cagcttcgat ttcacaacta
    28021 accgtttttc aacggcttat tactcatcat cccgtcccac ctcatccttg tatccccagc
    28081 ttccttccca tgcacgcacc tctcctatcc cccgcctccg cgccgtgtac aaggggtgta
    28141 caagggatgc cca
//
LOCUS       DAOPQG010000042        27612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2223_length_27612_cov_13.975215, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000042 DAOPQG010000000
VERSION     DAOPQG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 27612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27612
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2223_length_27612_cov_13.975215"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1902
                     /locus_tag="PKI07_06595"
     CDS             142..1902
                     /locus_tag="PKI07_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50760.1"
                     /translation="MVTLMPNASVARPLFRAADGLPGGSIRKIGIGALLLLLLTTGSS
                     AQSLRVTTWNLQPPAVRTNAAAVNAGDIHISAAAAELKKLNPDVILLQQVVDWAMCDA
                     LAQALKPAQYNVVACSAFREARTNALRKQQVAILAKPEARAYFSWTESWRNRGVPTVA
                     GGFAFAAFQVGNQRIGFFSVQAGAPTGLTPSKKRAAAAPEERLTPAIEQVLAQVRAIN
                     NWITNRVQACVIGGTFGRTTGQVMALEGAPLQMLESAGFGDAFQGVPAAGRATQRAKA
                     GQTGPVADYLFIQPPSCASDPVVQPVAAARHYPVACEVRLDLLKVVALPMAHVESAPP
                     RKAPPAQRQASSARALSQSDESVPVLPPPGVRVPSLLWLAVAVAGLAILAVVFRLAFR
                     RKRLPAPAEPALLPAGSEAPSGYTVVLTSQSASESALPGREPARAPRPLIHMEARETT
                     HTQTELWRQRVQAAERRAERADAVIRAGLVPHLRRWLKQKLVRRLISDRTQMLETQEA
                     AACKAQAVEQRLARIEQQIQQQERVYQERLAELTRELLAAKEENCELIRARIDQVKAE
                     MATARARLVAQSHANREGVE"
     gene            complement(1931..3250)
                     /locus_tag="PKI07_06600"
     CDS             complement(1931..3250)
                     /locus_tag="PKI07_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOC50761.1"
                     /translation="MPSLTRNTKALAGSETPELPLDYDPRRKTVRRPPLAEELAAFRE
                     FNQATRTLTTVCRDADGTIREVPTYVNEFWTAKQRQASSLQEISYRACFKPQLPRFFI
                     ERLTQPGETVYDPFMGRGTTLIEAALLGRVPYGCDINPLSVVLTRPRLCPPRLEDVAQ
                     RLQQINFASGTAYPEELLVFFHADTLCALTALKQYLLARRAAGLLDAVDDWICLVALN
                     RLTGHSAGFFSVYTLPPNQAVSVKSQRKINARRNQTPPPRNVPTLILRKTRQLLRDCD
                     AAARRTLAKVAPRAILLTQPAAHTPQIPERSVSLAVTSPPFLGIVDYATDNWLRCWFL
                     GIDAQSVEFTVPRKLGAWQTAMTAVLRELHRLLKPGGHVAFEVGEVHAGKIKLEEALL
                     PCGVAAGLTPELVLINGQQFTKTANCWGVDNMAKGTNTNRVVLFRRS"
     gene            3539..4201
                     /locus_tag="PKI07_06605"
     CDS             3539..4201
                     /locus_tag="PKI07_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOC50762.1"
                     /translation="MILASPLQGEVIYDNSKNDLLARYSPGEVEIGDEIFLDGTARFL
                     TYFSFEWWGTSDDPNAFAGEVEARVRFYKNNGPDYHSPGGDFPTPGEVFYDSGWFGEF
                     GPTPRATFIFTPGMDFPIGGLWIPVDRMTWSVQFRGMGPGDALGVDIYCPPVVGANPN
                     DYWENDPVRGWRLLINPYAPNNQMNFAAYMEAVPEPSGMALLLVGGLGLMAQARRQRR
                     KG"
     gene            complement(4278..5660)
                     /locus_tag="PKI07_06610"
     CDS             complement(4278..5660)
                     /locus_tag="PKI07_06610"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOC50763.1"
                     /translation="MLATGFLATMMAALPLRAPAQPSLHVIRTNGPASNRLNIVVLAE
                     AYTSAQLGQFLVDATNAVATLLSHPPYTGYSNYVNAFAIKMASKESGSDHPKYGISKD
                     TYFNSAYDSYWDELITIPSGTNGQGRVTALLQGFMPQCHLAILLVNDPKPGGSDGFNL
                     TAIVSTSAVAAETAMGQPAILTHEIGHVLANLGDEYTTPYPGFPDLEEPNTTRETNRA
                     AIKWNAWISPETPIPTPDSYGDGIVGLFTGAHYHETGWYRPQLNCAMGVMGVPFCAVC
                     REALVLAIYQRARPVDAFWPASTNLSVSTTQALSFSLVLQQPTFHNLTVQWCTNGVPL
                     SSATNSTFTLLPQFLSPGSNQVAARVKDNTPWVRTDPTNLLSQTIAWTVNVSLPQLRL
                     DAPVRLPGGRLAFRVTGTAPQGVVLQSSSNLISWSRLSTNSLEAGQFWHTNTHPGAYP
                     WKFYRAVTPP"
     gene            complement(5737..8457)
                     /locus_tag="PKI07_06615"
     CDS             complement(5737..8457)
                     /locus_tag="PKI07_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOC50764.1"
                     /translation="MAATFIVSNTNASGAGSLQQAILSANATSGLDTIVFQIPGSGVH
                     TISPANALPTITDPVVIDGTSQPGYGGTPLIELNGANAGTSSDGLNVTAGGSTIRGLI
                     INRFYGAGLSIQAPGSSNVIQGNYIGTDRTGTISQGNGRATTRMGGVLVQGSSGNVIG
                     GTNSAHRNVISANGGTGIYLHNSSGNTVQGNRVGTCVSGMTALGNTTNGIGLYNAGGN
                     LIGGTSAAARNVVSGNNQSGIYLYGAGSTGNRIQGNYIGTDASGHAGLGNGATGVLLN
                     GSSDNTIGGTQAGAGNVICRSGFFGVEITGGGSNNLVQGNFIGTDASGGAVLGNCGSG
                     VCLSQANSNLIGGTVPTARNLISGNALIGILITNGVGNVVAGNLVGTDVSGAQRLGNL
                     MAGIRIFGANSNRIGGALAGARNLISGNAHSGLEIMAGATGNLVQGNYIGTDLAGHLA
                     VSNGVDGIHIESPRNTIGGTVSGAGNLISGNRTNGIFLTGNQAAGNLIQGNFIGTTAD
                     GKAGLMNYWAGIGISHAPGNLIGGTTPGAGNLVSANAIADGDAGIFLIGSGAKGNVIQ
                     GNKLGTDITGTLSLGNRHEGVYLENAPSNTIGGITAAAANLISGNQTWGLYLVNSSWN
                     VIQGNLIGTKADGISALGNIFHSVECETGANNNMIGGASGAANTIAYAQGIYSGVRIR
                     NVSYNNAILGNRIFGNGALGIDLGNYGVNAIIPCGSTSGGNLSQNYPVLAQAVSGTAT
                     AIRGMLNCKPNQPYVLQFFANPACDPSGYGEGQFYLGQITVVTGANCTNSFVAHLPTP
                     VPPGYSTITATATDSANNTSEFSACIPVGPVPTLEIEALANPQVRISWTNTAAGFVLK
                     ETTSLSPPIHWQTVSNTPVLSNGQFVVTLPLNPSNPALRQGNRFFLLSFE"
     gene            9112..10404
                     /gene="purD"
                     /locus_tag="PKI07_06620"
     CDS             9112..10404
                     /gene="purD"
                     /locus_tag="PKI07_06620"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOC50765.1"
                     /translation="MKILVIGSGGREHALVWKLAQSPHVEQMWCAPGNAGISEERLAK
                     SGKQVENVPLGAEDLTGLRAFAQQHRPDLTVVGPDNPLALGIVDLWQKEGHRIWGPNQ
                     QAARFESSKVFAQQFMEKHGIPTARAGVFTDFAAASRFAATLGGRCAVKADGLALGKG
                     VLICRDMAQADHAIEEILVKGAFGSAGARLVIQELLEGMEISLHALCDGKAAKLFPTA
                     QDHKRALEGDRGLNTGGMGAYSPAPFLTAPELTAVGRRILDPWLRGCAAEGIDFRGIL
                     YPGIMLTQEGPKVLEFNARFGDPETQVYLPRLKNDLVELLEASVDGTLAEIALEWDPR
                     PSVCVVMASGGYPGSYQKNKPIRGLEAAARMANTKVFHAGTARVGDQVVTSGGRVLGV
                     TAWGEDLKRARDAAYAAVDAIGFDDAHFRRDIAAKALA"
     gene            10618..12147
                     /gene="tilS"
                     /locus_tag="PKI07_06625"
     CDS             10618..12147
                     /gene="tilS"
                     /locus_tag="PKI07_06625"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOC50766.1"
                     /translation="MSELRDHVARVIREHRLLRPGQRVLVAVSGGVDSLVLLRVLEEL
                     ARGQRWRLTVAHLNHQLRGRSSDADERLVCREAARLKLPVVVERVAVGRLGRAQGGSL
                     EMAARKARHDFLAGTAARLRIPTIALAHQADDQVELFFLRLLRGSGGEGLAGMKWRGP
                     SPSNPGVELIRPLLDQPKSALCQFAREQGIRYREDASNARLDFQRNRIRHELLPLLRR
                     NYQPALDRAILRLMAIIGDESEFVGAEAGRWLEGGAGVGAERAGSKGRGGKGARRPLS
                     PGARSASRRLRFEELPVAVQRRCVQMQLARQGVVPDFQRVEELRLKAGKKVAVGVAAA
                     AGDGAHRSPQRKDERAARSEPIRYAVRDSWGEVRVEEGATEMFGTGSRQVELRGRAGE
                     LEFGGVRICWRLRSGGNYRRGQRSEGKEYFDADLVGSPVIVRHWRPGDRFQPIGMAGS
                     VKLQDFFTNQKVPRDQRRRLVVGVNAAGEIFWVEGMRIGERGKLHRGTIRRLQWCWKR
                     L"
     gene            12205..14223
                     /gene="ftsH"
                     /locus_tag="PKI07_06630"
     CDS             12205..14223
                     /gene="ftsH"
                     /locus_tag="PKI07_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOC50767.1"
                     /translation="MANDNKSGDPGKNLNKGKEFKVPPRTMMVWIAILGGVTLLMLFK
                     DRMETPGELLSQYKFQQLAQSNQIVRATINYSPQSPDLREITGKYWRLEGEHKVEVPF
                     RVKARLTPRLEEMLLGMPQFEPREPSTMLYSFMLSILPFLVIAALIYFFFIRQIKMAG
                     KGALSFGKSKARLLAKERNKTTFKDVAGVEEAIQEVSELVEFLKDPKKFQRLGGRIPK
                     GVLMIGPPGTGKTLLAKAIAGEADAAFFSISGSDFVEMFVGVGASRVRDMFEQARRNT
                     PCLVFIDEIDAVGRSRGHGLGGGNDEREQTLNALLVEMDGFDTQEGIIIIAATNRPDV
                     LDPALLRPGRFDRQVTVNLPDVRGREAILKVHAKSVKLSPAVDLSVIARGTPGYSGAE
                     LANLLNEAALLAASTNKKAVGMEELEEARDKVRWGRERRSLAMTDEEKKFTAWHEAGH
                     ALLNVILQHTHPLHKVTIIPRGQALGATMSLPKTDVLNRRRKEMLDLIAVMMGGRIAE
                     EIISGDISSGAAGDIQQATNMARAMVTQWGMSDRLGMVQYGNDDEFVFLGREVSRAKV
                     YSEFTAQEIDSEVKLIIDSAYQRAKDLLTTNLDKLELIANTLLEYETLDGQQVEEIVR
                     TGKFTAPPPAPEVEPPRGAPAGTPLPETPAKPAPPKLPGLGTPAPATA"
     gene            14351..15070
                     /gene="rpe"
                     /locus_tag="PKI07_06635"
     CDS             14351..15070
                     /gene="rpe"
                     /locus_tag="PKI07_06635"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOC50768.1"
                     /translation="MIIAPSVLGANFGRLAREVSRVNASGAEWVHLDIMDGHFVPNIS
                     FGPAVVQTLRPLSKLFFDAHLMCSKPEILLEPFARAGANQIDIHVELGEAVTPLLWKI
                     RSLGCKAGLVINPPTSIALVAPYLEEIDFLLIMTVNPGFGGQSFIHEMLPKIQKAQAW
                     RRERGLDFKIGVDGGVDFKTVAECARAGADTFISGSTLFGRPNLNAAVRKMRKAAEAA
                     VGQAGVAAPAGPEPAGARKAN"
     gene            15093..16508
                     /locus_tag="PKI07_06640"
     CDS             15093..16508
                     /locus_tag="PKI07_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOC50769.1"
                     /translation="MSIKFGTDGWRAIIADEFTFANVARVAQASADYWRVRRVSGTEK
                     KVVIGYDRRFLSDAFAARSAEVFAGNGFQVVLTPAPTPTPSVSYAVKSQRAIGGVMIT
                     ASHNPPSFNGFKLKAHYGGSAAPEECAGVEAWLDKRPVKATPLADAMKAGRIRIKDVR
                     PAHYAAIKRLVDFKRIARSGLRFAHEALFGVGAGCFEELLAGTSCRVTTLNARHDAFF
                     GGINPEPIEANYARSAAFLKQHPHDICLVTDGDADRVGGMDGRGNYLTTHQVICLLLH
                     HLIVNRQLRGRVVKALNTTSMMDAICAAYGLELVETGVGFKYIAAEMIKGGVLLGAEE
                     SGGIGFPGHIPERDGIAAGLVLLELLATERVSVHKILGSLVKKFGPHEYGRIDTRFPL
                     EKRAELMDFLKRNPPDRLLRSGVVEVKTYDGVKFIARDASWLMVRGSGTEPILRIYAE
                     AGSAAAVRKLLQLGVRLTRQI"
     gene            16959..18791
                     /locus_tag="PKI07_06645"
     CDS             16959..18791
                     /locus_tag="PKI07_06645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOC50770.1"
                     /translation="MAGDKKHVYDESKIKTLSSLEHIRLRTGMYIGRVGDGSHYDDGC
                     YILLKEVIDNAIDEFIMGYGKEVQITIQDRAVSVRDFGRGIPLGKVVDCVSKINTGAK
                     YNDDVFQFSVGLNGVGTKAVNALSKVFRVCSYREGQFAEASFKQGKLKKENSGKAPRE
                     ADGTLIEFEPDPEVFKEVEFRPEHVERRLRHYSYLNSGLKLRYNGKTFQSRNGLFDLV
                     MEDLQADGSEPIYPPLHYVEKTLEFCFTHSNSRYGETFYSFVNGQYTSDGGTHLSAFR
                     EGLLKAVNEYAQEKFDGDDVRESMVGAVSIRLKDPVFESQTKNKLGNTEIRTELVNRV
                     REELLQFFNRNKEIAGKVVAKVQDTRQLRKELQAVKKLARERAKAITIRIPQLKDCKE
                     HYNRAKGKGKGTMIFITEGQSAAGSIISCRDVETQAIFVLKGKPLNVWDLKRDVVYKN
                     DELYNLMSSLDIEDRLEGLRYEKVILATDADVDGLHIRNLMITYFFRFFDQLVRDGHL
                     FVLETPLFRVRNKEQTLYCYSEGERDAVAQKLGAGCEITRFKGLGEISPAEFKQFIGK
                     NMRLSQVEYAPKHDAAGILGFYMGKNTPERKDYIMENLVVPAEE"
     gene            19000..21159
                     /locus_tag="PKI07_06650"
     CDS             19000..21159
                     /locus_tag="PKI07_06650"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOC50771.1"
                     /translation="MSARRKNNADQPELPIQVPPDNTPGVVNAAPVPEGGATEAPRRG
                     SARKGNEATHVLAEKVALPAPARSFVPGKIELPLHRRVDRNFLDYASYVIRDRAIPNL
                     ADGLKPVQRRILWALHDKDDGRFIKVANVVGHTMQYHPHGDASIGDALVVLANKRYLI
                     EGQGNFGNLLTGDLAAAPRYIECRLTELARTELFNDEITELVPSYDGRNQEPVALPSK
                     LPLLLMLGAEGIAVGMSSRILPHNFPELLEAQIAILKKQPFKCLPDFQTGGLMDARGY
                     KDGAGEIKVRARLRVKDESTVVIKEIPPTTTTDSLIGSIEDASRKGKIKVRSINDFTS
                     EEVEIEVKAPSGVSAEQLADALYAFTDCEVTISSRIVVIRNNRPVELTVSEVLRENTA
                     QLVEILKRELELKETHLQDELHFRTLERIFIEERLYKKIEQCRTNEAVFTAVREGFKP
                     FRRQLIRELTEPDIERLLTIRIRRISLFDINKHREEVERVKIELAEARKNLKHLTRYV
                     IGHLEALLAKYRPLYPRLTRSSRYDEVDAKEVAFKAFKVAYDRASGYVGHKVSGSEFQ
                     VECTRFDKLIFVFKDGHYQVSELPEKLYVGPDLIYCGLPERDRVFTCAYTTREATYLK
                     RFTFGGAILNKVYRCIPPKARVLFFEPDTPSELYIRYKPAPYQKINQQTCRPAEVEVR
                     GPKTRGRQVSIKDVSSITSQPTRGWDPEAPTTRLQFA"
     gene            21315..22133
                     /locus_tag="PKI07_06655"
     CDS             21315..22133
                     /locus_tag="PKI07_06655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOC50772.1"
                     /translation="MKLSLLFGRGLPGVAIVLLSALLFTGCATVPETGRSQLNLISAA
                     EEMKLGLTEFEKTKQSIPISKDAKAAAMVQRVGQRIAAVANLPNAQWEFVLFDSAEAN
                     AWCLPGGKVGVYSGILPITKDEGGLATVIGHEIAHATAHHGAERMSQLMAAQLGESAV
                     GALTSGSSQLTQALVGQAYGLASQVGVLLPYSRKQEAEADYIGLLYMARAGYDPMAAV
                     AFWERFSAYNEQHGGNREFSFLRTHPLDQQRIQNLKAKMPQAQAEYQRAKAAGK"
     gene            complement(22142..24334)
                     /gene="fusA"
                     /locus_tag="PKI07_06660"
     CDS             complement(22142..24334)
                     /gene="fusA"
                     /locus_tag="PKI07_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOC50773.1"
                     /translation="METASETRRSPKTKGPVVPQRQYPIEHTRNIGIAAHIDAGKTTT
                     TERILFYTGLIHKMGDVDDGNTVTDWMDQERERGITITSAATTSFWTQKADGLYKAFA
                     GIPHRINIIDTPGHVDFTAEVERSMRVLDGAVAVFCGVAGVQPQSETVWRQATKYKVP
                     RIAFVNKMDRVGANFMNALADMRKKLGAYAYPIFLPIGKEDHFDGVVDLVNQKAIIYD
                     PNDEHGLNCQVTDIPAALKEAAAMALAELIDAVSNKDDRIAELVIENQPVTPEALKAA
                     IRRLTCKLELVPVLCGSAFRKRGVQALIDAIVDYLPSPLDIPPAVGITPDTEAKVEVY
                     SDDNGKFCSLAFKLWTDPYVGKLVFFRVYSGRLSKGDVIYNPRTRKRERVSRVLMIQA
                     DKRIDVETVFAGDIAALVGVKNITTGDTLCDEDFEALLEPPTFPEPVISMAVEPKTKA
                     DREKMSEGLQRLAEEDPTFRCFTNEETGQLIIAGMGELHLDIIRNRLFREFKVSANVG
                     APQIAYRETVTKAAEGEGKFIRQSGGRGQYGHACITLAPNERGKGIEIENKIVGGAIP
                     KEYIPAVIDGIKEAIAGGVLAGYAVVDVKVAIVDGAFHEVDSSELAFKMAGIFAFKEA
                     AQKANPILLEPIMKVELTTPDEYQGDLLGDLNRRRGKILQIEAKDTSTIATAEVPLAE
                     MFGYATAIRSLSKGRAAYSMEPFRFEQVPSNIVTGILALTKDIPPARA"
     gene            complement(24405..24878)
                     /gene="rpsG"
                     /locus_tag="PKI07_06665"
     CDS             complement(24405..24878)
                     /gene="rpsG"
                     /locus_tag="PKI07_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOC50774.1"
                     /translation="MSRRRRAISRPIPKDAKFNSALVTRLVNTVMKQGNKSVAQRIVY
                     GALDELVAKNPAINPLEVLQRAVDNAKPRLEVKARRMGGATYQVPLEVPTDRQFSLAL
                     RWLVDLADARKGIPMKEALAAEILDAYQGQGNAIRKRDEVHKMAQANKAFAHFRW"
     gene            complement(24925..25341)
                     /gene="rpsL"
                     /locus_tag="PKI07_06670"
     CDS             complement(24925..25341)
                     /gene="rpsL"
                     /locus_tag="PKI07_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOC50775.1"
                     /translation="MPTINQLVRKGRSRLKHKSKSPALENSPFRRGVCIQVMTRTPKK
                     PNSAMRKVAKVRLTNGYEVIAYIPDEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGTL
                     DAVGVEKRRVSRSKYGVKRPKAGARPAAAAAAAPAA"
     gene            complement(25777..27444)
                     /gene="lnt"
                     /locus_tag="PKI07_06675"
     CDS             complement(25777..27444)
                     /gene="lnt"
                     /locus_tag="PKI07_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOC50776.1"
                     /translation="MRSRCLLAVLAGLLLAASFPKPGLAGLAWVAPALLLGAALGKRG
                     WESFRIGYLGGLAYYLASLYWLLLIPYRWHSIPLGPALGWVSLSAYVALYPAAWVWLL
                     TMSLSRNTNTSARFGFLRKRPEPRLAGTLPWPGGAVNVLACSWGRRTLWAIGGAALWV
                     ALEMIRARLFTGFPWNPLGASQYQLIPLIQVASIAGVYGVSFLVVWTSLSLLSAGLMV
                     VSRPTSRSTWAGEILLPAAAIAAALHFGFRQLAFEPPPLRSLKVTLIQPSIPQTLIWD
                     SSRDWERFQEVLQLSRLALTNRTDLLIWPESAIPNLAQWDADIYTALTNLVRQHHVWL
                     IIGSDDAAPSTRPSRDNELEFYNASFLLSPEGEFVARYIKRNLVIFGEYIPLARWLPF
                     LKWFTPIGSGFTPGDRAVPFALTTPKVKTSTLICFEDTFPGLARTAVDEDTDFLVNLT
                     NNGWFGESAAQWQHAATAVFRAIENHRPLIRCSNNGLTGWVDAHGRLRQVFYDARGTI
                     YGPGFLIADIPLLTPDQPHPPTPYRQYGDRFGWACLALAGLLLAARL"
BASE COUNT         5373 a         7648 c         9245 g         5346 t
ORIGIN      
        1 cgcctgcccg tccacgagtg ccccggcttt ggaaaggccg ctcggaaatg gccgtgcgtc
       61 cagagatagc gaaatagcaa acgctccttg acagccctct gcctgacggg ttgagtggag
      121 tgcgttattt agcagccaca aatggtgacc ctcatgccga atgcgtctgt tgccaggccg
      181 ctcttccggg cggcagacgg tttgccgggc ggaagcattc gcaaaatcgg gattggggcg
      241 ttgctgctgt tgctgttgac gacaggcagt tctgcgcaaa gcctccgcgt taccacctgg
      301 aacttgcaac caccggctgt tcgcaccaat gccgcagcgg taaatgccgg ggacattcat
      361 atttccgctg ccgccgctga actcaagaaa ctcaatcccg acgtcatact tttgcagcag
      421 gttgttgatt gggcgatgtg cgacgcgctg gcccaggcac tcaagcccgc ccaatacaac
      481 gtcgtggcct gctccgcctt tcgggaggcg cggaccaacg cgctccgcaa acaacaagtg
      541 gcaattctcg ccaaacccga ggcaagggcc tatttctcgt ggacggaatc ctggcgcaac
      601 cgcggtgtgc caacggtggc ggggggattc gcctttgccg cgtttcaggt ggggaaccag
      661 cggattggtt tcttttcggt tcaggcaggg gcgcccactg gcctcactcc gagcaagaag
      721 cgcgcggccg cagcccctga ggaacggctg acacccgcca tcgagcaagt gctggcgcag
      781 gttcgcgcga ttaacaactg gattaccaat cgggtgcagg catgtgttat tggtggcacg
      841 tttggccgga cgaccggcca agtgatggca ctggaaggcg cgccgctgca aatgctggag
      901 agtgcgggct tcggcgatgc ctttcagggg gtgcccgcag ccggcagagc gactcagcgg
      961 gcaaaggctg ggcagacagg ccccgttgct gattatctct tcattcagcc gccctcctgc
     1021 gccagcgatc ctgtggttca gccggtagct gccgccaggc actacccggt tgcttgcgaa
     1081 gtgcggctag acctcttgaa ggtggttgcc ctcccgatgg ctcatgtaga atccgccccg
     1141 ccccgcaagg cacccccggc acagcgccag gcctcgtcgg ccagggcttt atcccaatcc
     1201 gacgagtctg tccccgtttt gccaccgccc ggcgttcgtg ttccgtcgct gctttggctg
     1261 gcggtggcgg tggctggcct ggcgatcctg gcagtggtgt ttcgactagc cttcaggcgc
     1321 aagagattgc cggcgccggc cgagccggcg ttgctgcctg ctggcagcga agccccgtca
     1381 ggctacacgg tggtgctgac ctcgcaatcg gcgtctgagt ccgctttgcc cggccgcgaa
     1441 ccggcgcgtg cgccccgtcc gctcatccat atggaagcgc gggaaaccac gcacacccaa
     1501 accgagctct ggcgccagcg cgttcaggcg gccgaacggc gggcggagcg cgccgacgcg
     1561 gtaattcggg cagggctggt gccccatctg cgccgctggc tgaagcagaa actggtgcgc
     1621 cggctgattt cggatcgaac gcagatgctg gagacgcagg aagccgcagc ctgcaaggcc
     1681 caggccgtcg agcagcggct tgcccgcatc gagcagcaaa tccagcagca ggaacgggtt
     1741 taccaggagc gcctcgccga actcacccgc gaactgctgg cggccaaaga ggaaaactgc
     1801 gagctgattc gcgcccgcat tgatcaggtc aaagccgaga tggccaccgc ccgggcgcgc
     1861 cttgtggcgc aatcccacgc caaccgcgag ggcgtcgagt agggcgggtg ggcgggagcc
     1921 ggacgcgggg ctacgaccgg cgaaagagaa caacgcggtt ggtattggtg cctttggcca
     1981 tgttatccac gccccagcag ttggccgtct tggtgaattg ctggccgttg atgagcacca
     2041 gttccggcgt caggccggcg gcgacgccac acggcagaag cgcctcttcg agctttattt
     2101 ttccggcgtg gacttccccg acttcaaaag cgacgtgacc gcccggcttc agcaggcgat
     2161 gcagttcgcg caggaccgcg gtcatcgccg tttgccaggc gcccagcttt cggggaacgg
     2221 taaactccac tgattgagcg tcaatgccca gaaaccaaca gcgcagccag ttatcagtgg
     2281 catagtccac tatgccgagg aatggcggcg aggtgacggc caatgaaacg gagcgttcgg
     2341 ggatttgggg cgtgtgcgcg gcgggctgcg ttagcaaaat ggcccgaggc gcgactttgg
     2401 ccagggttcg gcgggcggcc gcatcgcaat cgcgcagcag ttggcgggtc tttcgcagga
     2461 taagggtggg gacattccgg ggcggcgggg tttggttgcg gcgggcgttg attttgcgct
     2521 gggatttgac ggagacggcc tggttgggcg gcaaggtata gacagagaaa aagccagccg
     2581 agtggccggt cagccggttg agcgcgacca ggcaaatcca gtcatccacg gcgtcgagca
     2641 agccggcggc gcggcgcgcc agcaagtatt gcttgagcgc ggtgagagcg caaagcgtat
     2701 ccgcatgaaa aaagaccagc aactcctccg gataggcggt tccggaagca aagttgattt
     2761 gctggagccg ctgggcaacg tcctcaagac ggggagggca caaccgtggc cgggtcaaaa
     2821 caacgctcaa aggattgata tcgcatccgt atggcacgcg ccccagcagc gcggcttcga
     2881 taagggtggt gccacgcccc atgaacgggt cataaaccgt ttctccgggt tgtgtcaggc
     2941 gttcgataaa aaagcgcggc aactgcggtt tgaagcaggc gcggtaggaa atctcctgaa
     3001 gcgagcttgc ctggcgttgt ttggcggtcc agaactcgtt gacataggtt ggcacctcgc
     3061 ggatggtgcc gtcggcgtcc cggcagacgg tggtcagtgt gcgggtagcc tggttgaact
     3121 cgcgaaacgc ggccaactcc tcggccagcg gcgggcggcg aactgttttg cggcgcgggt
     3181 cgtaatccag cggcaactcg ggagtttccg agccggccaa ggctttagtg ttgcgcgtta
     3241 atgacggcat ttgccgggca tcaaaatgcc cttcggcggc tgttccgggc aagtgaagtt
     3301 tgcgacagaa aacgccgctc tgaccccgcc gtttgtaact gcaggaatct caaggcatta
     3361 tcgcccttcc tcccacaggc ttgccgctaa tatatttaat gaatccggtt gacaacccta
     3421 acccttttgg taaaatcaac tccgttggtt agtatgattg gtctgattac atcgtaaagt
     3481 gccttttatg caaacatcta gtaaaataaa ccttctctgg gccctaagct tgctgtgtgt
     3541 gatcttagct tctccgcttc aaggagaggt catctatgat aattccaaaa acgacctgct
     3601 ggcgcgctac agcccgggcg aggtggaaat cggcgatgag attttcctgg acggcaccgc
     3661 gcggttcctg acgtatttct cgttcgagtg gtgggggacc agcgacgatc ccaacgcttt
     3721 cgccggcgaa gtggaagcgc gagtgcggtt ttacaagaac aacgggccgg attaccacag
     3781 ccccggcggt gatttcccca cccccggcga agtgttttac gacagtggct ggttcggcga
     3841 gtttggcccg acgccccggg ccacgtttat tttcacaccg ggaatggatt ttcccatcgg
     3901 gggactttgg attccggttg accgcatgac ttggagcgtc cagttccgtg gaatggggcc
     3961 gggggatgcg ttgggggtgg atatctattg cccgcccgtc gtgggggcga atcccaatga
     4021 ctattgggag aacgaccccg tgcgcggctg gcggctgctg attaatccct acgcaccgaa
     4081 caatcagatg aatttcgcgg cctacatgga agcggtgccc gagccttccg gcatggcgct
     4141 attgctggtt gggggattgg gtctgatggc ccaggcgcgg cggcagcggc gcaagggata
     4201 gggcaaggac aggccgatgg ttttaccggc gcgggcggaa gctcgcgcct ttttgttggg
     4261 tgggctgacg ccgaagctta cggaggcgtg acggctcgat agaatttcca gggataggct
     4321 ccgggatgag tgttcgtgtg ccaaaactgc cccgcctcca gggaattggt ggacagcctg
     4381 ctccaggaga tcaggttgga ggaactctgg aggacaactc cttgaggcgc ggtgccggtg
     4441 acgcgaaaag ccaggcgtcc gcccggaagc ctgaccggcg catccaaccg caactgtgga
     4501 aggcttacat ttacagtcca ggcgatcgtt tggctcagga ggttggtcgg gtcggtgcgc
     4561 acccacgggg tgttatcctt gacccgggcg gcgacctggt tgctgccagg ggaaagaaat
     4621 tgcggcagca gcgtgaaggt ggagttggtg gcgctgctga gaggcacccc gttggtgcac
     4681 cattggacag tcagattatg gaaagtcggt tgctggagga ccaggctgaa tgagagggcc
     4741 tgggtagtcg agacggacag attcgtgctg gcgggccaaa aggcgtccac cggccgcgct
     4801 cgctggtaga tcgccaggac cagggcttca cggcaaacgg cgcagaacgg aacccccatt
     4861 acccccatgg cgcaattaag ttgcgggcgg taccacccgg tctcgtgata gtgagcgccc
     4921 gtgaagaggc caacaatgcc gtctccatag gaatccgggg tgggaatggg agtttcggga
     4981 gaaatccacg cgttccattt tatggcggcg cgattggttt cccgggtggt gttgggttct
     5041 tcgagatcgg gaaagcccgg ataaggcgtg gtgtattcgt cgcccaggtt ggccagaaca
     5101 tgcccgattt cgtgggtcag tatggccggt tgccccatcg cggtctcggc cgccaccgcg
     5161 ctggtcgaga caattgccgt cagattaaag ccatccgagc cgccgggctt gggatcgttg
     5221 accaggagaa tcgcaaggtg acactgcggc atgaagccct gaagcagggc cgttactctg
     5281 ccctggccat tggtacccga agggatggta ataagctcat cccaatacga atcgtaggcg
     5341 ctgttgaagt aggtgtcttt ggaaatcccg tatttgggat gatcggaacc cgactccttc
     5401 gaagccatct tgatcgcgaa ggcattgacg taattgctat atcccgtata cggcgggtgg
     5461 gatagaagcg tggcgactgc attggtcgca tcaacaagaa actgtccgag ttgcgcgctg
     5521 gtataggctt cggcaaggac gacgatgttc agccgattgg aagccgggcc attagttcga
     5581 atgacatgca gggagggttg cgcgggagcg cgcaacggca gggcggccat catcgtggcc
     5641 agaaagccgg tggccagcaa agaaaacgcg cggggcctgg cgcggcgcct cacggagggc
     5701 tggcgcagga gaggaaaaaa cgggaaccgg gtcacgctat tcgaaactca gaaggaaaaa
     5761 ccgattgcct tgccgcagcg ccgggttgga cgggttcagc ggcagggtga ccacaaactg
     5821 gccgttgctc aggacggggg tattgctcac cgtctgccag tggatgggcg gcgacaggct
     5881 ggtggtttcc ttgagcacaa atcccgccgc cgtgttagtc caggagatcc tgacctgggg
     5941 attggccagt gcctcgattt cgagcgtagg caccgggcca acgggaatac aggcggaaaa
     6001 ctcggaagta ttgttggcgc tatccgtcgc ggtggcggtg atggtggaat accccggagg
     6061 gaccggggtg gggagatggg cgacaaagct gttggtgcaa ttagcgccgg tgacaaccgt
     6121 tatctggccc aggtagaact gcccctcccc gtagccggac gggtcgcagg ccggattcgc
     6181 gaagaactgg agcacatagg gttgattggg tttgcagttg agcatgccgc ggatggcggt
     6241 ggcggtgccg ctgacagcct gggcaagcac cgggtagttt tgcgacaagt tgccgccgct
     6301 ggtgctgccg cagggaatga tggcattgac cccgtaattg cccaaatcaa tgcccaaagc
     6361 gccgttgccg aaaatccggt tgcccagaat ggcgttatta tagctgacgt tacggatgcg
     6421 gacgccggag taaataccct gggcataagc aatcgtgttg gcggcgccgc tagcgccccc
     6481 gatcatgttg ttgttggcgc cggtttcaca ttccacggag tgaaaaatat tgcccagcgc
     6541 gctgatgccg tccgcttttg tcccgatcag gttgccttgg attacgttcc aggatgagtt
     6601 gaccaggtaa aggccccagg tttggttgcc cgagataagg ttggcagcgg ccgcagtgat
     6661 gcccccgatc gtgttagagg gggcgttttc gaggtaaact ccctcatgcc ggttgcccag
     6721 cgacagggtg cccgtgatgt cggtgccgag tttgttgcct tgaatgacgt tccctttcgc
     6781 cccgctgcca atcaggaaaa tcccggcgtc gccgtcggca atggcgttgg cggagaccag
     6841 attgccggct ccaggggtgg tgcccccgat gaggttgccg ggagcgtggg agatgccaat
     6901 ccccgcccag tagttcatca accccgcctt gccgtcggcc gtcgtgccaa taaagttccc
     6961 ctggatgagg ttcccggcgg cttgattgcc ggtgaggaaa atcccgttgg tgcggttccc
     7021 ggaaatcagg ttgcccgcgc cgctgacggt cccgccgatg gtattgcggg gagactcgat
     7081 gtggattcca tccactccgt tgctcacggc caagtgcccg gccaagtcgg tgccgatgta
     7141 gttcccttgc acgaggttgc cggtggcgcc cgccatgatc tccagcccgg aatgggcatt
     7201 gcccgaaatg aggttgcggg cgccggccaa agctccgccg atccggttgg aattggctcc
     7261 aaagatgcga atgccggcca tcagattgcc caggcgctga gcgccggaga catcggtgcc
     7321 aaccaggttg cccgccacca cgttgccgac gccgttggtg attagaatcc cgatcagcgc
     7381 attgccggag atgaggttgc gcgccgtggg aaccgtgccg ccgatgagat tgctgttggc
     7441 ttgcgagagg cagacgcccg agccgcaatt gccgagaacc gcgccgccgg aggcgtcggt
     7501 gccgatgaaa ttgccctgca cgaggttgtt cgagccgccc ccggtgattt ccaccccaaa
     7561 aaagccgctt cggcaaatga cattgcccgc ccccgcctgg gttccgccga tcgtattgtc
     7621 gctgctgccg ttcaacagga cgccagtggc gccgttgccc aggccggcgt ggccgctggc
     7681 atccgtgccg atatagttgc cctgtatccg gttccccgta ctgccggcgc catacaaata
     7741 gatgccgctc tgattattcc ccgagacgac gttgcgcgcg gcggcggacg tgccgccgat
     7801 caggttcccg ccggcattgt aaagcccgat gccgttggtg gtattgccca gggccgtcat
     7861 gccggagacg catgtgccga cccggttgcc ctgaaccgtg ttgccgctgc tattatgcag
     7921 gtaaatgccc gtgccgccgt tggcggagat gacattgcgg tgcgccgaat tcgtgccgcc
     7981 gatgacattg cccgaggagc cttgaaccaa cacgccgccc atgcgcgtgg tggcccggcc
     8041 gttgccctgg ctgatggtgc ccgtccggtc ggtgccgata taattgccct gaatgacatt
     8101 cgagctgccg ggcgcctgaa tggaaaggcc ggcgccgtaa aagcgattga taatcaaccc
     8161 gcggatcgtg ctcccccccg ccgtcacgtt gagaccatcg ctgctggtgc cggcattggc
     8221 gccgttaagt tcgatcaacg gggtgccccc gtagccgggc tgtgaagtgc catcaatgac
     8281 gacgggatcg gttatggtcg gcaaggcgtt tgccggcgag atggtatgca ctccggagcc
     8341 ggggatttgg aaaacaatcg tatccagccc gctcgtggca ttagccgaaa gaatggcctg
     8401 ttgcagcgac ccggccccgc tggcgttcgt atttgagact ataaaggtgg ccgccatgcc
     8461 gcgggaaagg ctaaatcctg ccaatatcgc caggaggctc agcccatagg gaagacgact
     8521 tattcgcaag aaagatgccc gggttttgtc catcataaat gctgcgctaa ccagcctaca
     8581 ctatcacacc gacaccggga gacaagcaga aacggcccca gcaggaaaaa gccgcgcggc
     8641 ggcaggggaa gacccttgct tatttgcggc ttaagggggc cgggcaggca aacggcggtt
     8701 ccaggaaccc tccaagctcc ttaccaagcc cccagccacc tccaaaagcc ccagcctgcc
     8761 cagccattct aaggctccca ataagccgtg tacaaggggt gtacaaggga tgctcaccgg
     8821 gtgtacatcg aaaaatctga tgagcatccg gtgagcatcc cttgtacacc ccttgtacac
     8881 ggcttgaaag gacgggggca gcagggggat gcgagaggaa gcgggctttg aggttggcga
     8941 tggggtttta tatctaacct aagtgttttc agtaacttgt gctatattaa gccgggacag
     9001 aggcaaaatg agggtgtttt tggccttttc cgatgctcct gggttgattg gcggcgaata
     9061 tttgcaaaag ggctttcgcc ccggggcgga ttcctgtttc aatgatcccc catgaaaatc
     9121 ctggtcatag gttcgggcgg gcgcgagcac gcgttggtgt ggaaactggc ccagtcgccc
     9181 catgttgagc aaatgtggtg cgccccgggt aatgccggca tatcggagga acggctggcc
     9241 aaaagcggca agcaggtgga aaacgttccg ctgggggccg aggatttgac gggtttgcgg
     9301 gcctttgccc agcaacaccg gcctgatctc acggtggttg gcccggataa tccgctggcg
     9361 ctgggcattg tggacttatg gcaaaaggag gggcaccgca tttgggggcc caatcaacag
     9421 gctgcgcgct ttgaatcgtc gaaggttttt gcgcagcagt ttatggaaaa gcatggcatt
     9481 ccgacggccc gggcgggggt ttttacggat tttgcggcgg cgtcgcgttt cgcggctacg
     9541 cttggggggc gttgcgccgt caaggccgac ggtttggctt tgggcaaagg ggtgttgatt
     9601 tgccgcgaca tggcccaggc cgatcacgcc atcgaggaga ttctggtaaa gggggctttt
     9661 ggcagtgccg gggcccgcct ggtcattcag gaactgctcg aaggcatgga gatctccttg
     9721 catgcccttt gcgatggcaa ggccgccaaa ctctttccca cggcccagga ccacaagcgc
     9781 gccctggagg gggaccgcgg tcttaatacc ggcggcatgg gcgcctattc ccccgcgccg
     9841 tttctgaccg cccccgaact gacggcggtt ggccgccgca ttctcgatcc ctggctgcgg
     9901 ggctgcgcgg cggaggggat tgattttcgg ggcattcttt acccgggaat catgctcacg
     9961 caagaggggc ccaaggtgct ggagttcaat gcccggtttg gcgacccgga gacgcaagtc
    10021 tatctgccac gcctgaagaa tgatctggtc gaattgctcg aggcgagcgt ggacgggaca
    10081 ttggcggaga tcgcattgga atgggacccg aggccttccg tttgcgtggt gatggcgtcg
    10141 gggggctacc cgggcagcta ccagaagaac aagcccatcc gcgggcttga ggctgccgct
    10201 cgcatggcca ataccaaagt cttccacgcc ggcaccgccc gggtgggaga tcaagtggtc
    10261 accagcgggg gccgggtgct gggggtgacc gcctgggggg aagacctgaa gcgggcgcgg
    10321 gatgctgctt atgccgcggt ggacgccatt ggctttgacg atgctcattt ccggcgtgat
    10381 atcgccgcca aagccctggc ttgagcgagc ccgggcagcc gcgcccggcg cggctgtcaa
    10441 gcgggcggcc gggccggggc tgatgctgcc gccgcgggcg ggagcggccg ccttcgtttc
    10501 gagggcgcgc cccggcgtcc ggcggccaac tccgcgagcc gggatgcagt tggtgaggcg
    10561 gcgtgggcgg ggcgggatca tccttgctta gtgaggccgc tgaaatagca tctgagcgtg
    10621 agcgaactgc gggatcatgt agcgcgagtg attcgcgagc accggctgct gcgcccgggg
    10681 cagcgcgtcc tcgtggcggt ttccggcggg gtggactcgc tcgtgctttt gcgggtgctg
    10741 gaggaactgg cgcgggggca gcggtggcgg ctgacggtgg cgcatttgaa ccaccagctt
    10801 cgcggccgga gcagcgacgc cgacgagcgc ctggtttgcc gggaggcggc ccggttgaag
    10861 ctgccggtgg tggtcgagcg cgtggcggtc ggccgactgg gccgcgcgca gggggggtcg
    10921 ctcgagatgg ccgcgcggaa ggcgcggcac gattttctgg ctggcaccgc cgcgcgcctg
    10981 cgcatcccca cgattgcgct ggcgcatcag gccgacgatc aggtggaatt gttctttctg
    11041 cggctgctgc gcgggagcgg aggggaagga ctggccggca tgaaatggcg cggcccctcg
    11101 ccgagcaacc ccggggttga gttgattcgc ccgctgctgg accagccgaa gagcgcgttg
    11161 tgccaattcg cgcgggagca gggcattcgc tatcgggagg atgcgagcaa tgcgcggctg
    11221 gattttcagc ggaaccggat tcgccatgag ctcttgccgt tgctgcggcg caattatcaa
    11281 ccggccctgg acagggcgat cctgcgcctg atggccatta ttggcgatga atcggagttt
    11341 gtgggggcgg aggctggccg gtggctggag ggcggagccg gggtgggagc cgaaagggcg
    11401 ggctcgaagg ggaggggggg gaagggcgcc cggcgacctc tttcgcccgg ggcgcggagt
    11461 gcgtcgcggc ggctgcggtt tgaggaattg ccggtggccg tgcagcggcg gtgcgtgcag
    11521 atgcagttgg cgaggcaggg agtcgtgccg gattttcaac gggtggagga attgcgcctg
    11581 aaggcgggga agaaagtcgc ggttggcgtg gcggcggcag ccggggatgg tgcgcaccgt
    11641 tctccgcagc gcaaggatga gcgggccgcg cggagcgaac ctattcgtta tgcggttcgc
    11701 gattcgtggg gcgaagtgcg ggtggaagag ggagcgacgg aaatgtttgg gacggggtct
    11761 cggcaggtgg aattgcgggg cagggcaggg gaattggagt ttggcggggt caggatatgc
    11821 tggcgtctcc gttcgggggg aaattaccgg cggggacagc gctccgaggg gaaagagtat
    11881 tttgatgcgg acctcgtggg gtcgccggta atcgtgcggc attggcggcc gggcgatcgc
    11941 tttcagccga ttggcatggc cggttcggtg aaactgcagg atttcttcac caatcagaag
    12001 gtgccgcgcg atcagcggcg ccggctggtc gtgggcgtga acgcggcggg cgagattttt
    12061 tgggtggaag ggatgcggat cggggaacgc ggcaagcttc atcgggggac gattcgccgc
    12121 ttgcaatggt gctggaagcg gctttaactg gcggagttgc ggctggccgg cgggtgtgct
    12181 aggcttgcaa cgaccagagc taagatggcg aatgacaata aatccggcga cccgggcaag
    12241 aacttgaaca aaggcaagga gttcaaggtg cccccgcgta ccatgatggt ctggatcgcg
    12301 attctcgggg gcgtcacgct gctgatgctc ttcaaggatc gcatggagac gccgggcgag
    12361 ctgctgagcc aatacaagtt ccagcagttg gcgcagtcga accagattgt ccgggcgacg
    12421 atcaattaca gcccgcaaag cccggatttg cgggagataa ccggcaagta ttggcggctg
    12481 gagggcgagc acaaggtgga ggtgccgttc cgcgtgaaag cgcggctgac gcccaggctg
    12541 gaggagatgc tcctgggcat gccgcagttc gagccgcgcg agcccagcac gatgctgtac
    12601 agtttcatgc tgagcatatt gccgtttctg gtgatcgcgg ccctgattta ttttttcttc
    12661 atccggcaaa tcaagatggc gggcaaaggc gcgctgagtt ttggcaaaag caaggcgcgg
    12721 ttgctggcca aggagcgcaa caaaaccacg ttcaaggatg tggcgggcgt cgaggaggcc
    12781 attcaggagg tgtcggagct ggttgagttt ctcaaggacc cgaagaagtt tcagcgtctc
    12841 ggcgggcgga ttcccaaggg cgtgctgatg attggcccgc cgggcacggg taagacgctg
    12901 ctggccaagg ccattgcggg cgaggcggat gccgcgtttt tcagcatcag cgggtcggat
    12961 tttgtggaga tgttcgtggg cgtcggcgcc agccgcgtgc gggacatgtt tgagcaggcg
    13021 cgccgcaata cgccctgcct ggtgttcatt gatgaaattg acgcggtggg gcggagccgc
    13081 gggcatgggt tgggcggtgg caatgatgag cgggagcaga cgttgaacgc gctgctggtg
    13141 gagatggacg ggtttgacac gcaggagggc attatcatca tcgccgcgac caaccggccg
    13201 gatgtgctgg atccggccct gttgcgtccg gggcggtttg atcgccaggt caccgtcaac
    13261 ctgccggatg tccgggggcg cgaggcgatt ttgaaagtcc atgccaagag cgtgaagctg
    13321 tcgcccgccg ttgacctgtc ggtgatagcg cggggcacgc cgggctattc gggggcggag
    13381 ctggccaacc tgctgaacga ggcggccttg ctggcggcca gcaccaacaa gaaggcggtg
    13441 ggcatggaag agctggagga ggcgcgggac aaggtgcgtt gggggcgcga gcgccgcagc
    13501 ctggcgatga cggacgagga gaagaaattc accgcctggc atgaagccgg gcatgcgctg
    13561 ctcaacgtga ttttgcagca cacgcatccg ttgcataagg tcacgatcat tccgcgcggc
    13621 caggccctgg gggcgacgat gtcgctgccg aagacggatg ttttgaatcg gcggcgaaag
    13681 gaaatgctgg acttgatcgc cgtgatgatg ggcgggcgga ttgccgagga gattatttcg
    13741 ggagacattt ccagcggcgc ggcgggcgac attcagcagg caacgaatat ggcccgggcc
    13801 atggtcaccc agtggggcat gagcgaccgg ctggggatgg tgcaatacgg gaacgatgat
    13861 gagtttgtgt ttctgggccg cgaggtttcc cgcgccaagg tgtatagcga gttcacggcg
    13921 caggagattg acagcgaggt gaagctgatc attgactccg cttaccagcg ggcaaaagac
    13981 ctcctgacaa ccaacctgga caagctggag ctgatcgcca atacgctgct cgaatacgaa
    14041 actttggacg ggcagcaagt ggaggagatc gtgcgcacgg gcaagttcac cgcgccgccg
    14101 ccggcgccgg aagtcgaacc cccgcgcggc gcgcccgcgg gcaccccgct gccggaaacg
    14161 ccggccaaac cggcgccgcc caagctgccc ggactcggca cccccgcgcc cgccacggcg
    14221 taaagattcg ttcgcccgaa aggagcaggc gccctgccgg aggagctgct gttgccgcgg
    14281 gagccagcct gtatcccggc gcgggaattc cgcattcgac atttgcgcag ccgctgatta
    14341 ggttccgggc atgattatag cgccatcagt gctgggtgcg aactttggcc ggcttgcccg
    14401 cgaagtcagc cgggtgaacg cgtcgggggc cgaatgggtg cacctggata ttatggacgg
    14461 ccatttcgtg ccgaacattt cgttcggtcc ggcggtagtg cagacgctgc gtccgctgtc
    14521 gaagttgttt ttcgacgcgc acctgatgtg ttcgaagcct gagattctgc tggagccgtt
    14581 tgcgcgggcg ggggcgaacc agattgacat tcatgtggaa ctgggcgaag ccgtgacgcc
    14641 gttgctgtgg aaaatccggt cgctggggtg caaggcgggc ctggtcatca atcctcccac
    14701 ctccatcgcg ctggtggctc cctatttgga ggagattgat ttcctgttga tcatgacggt
    14761 gaatcccggt tttggcgggc agtcgttcat ccacgagatg ttgcccaaga tacaaaaggc
    14821 gcaggcctgg cggcgggagc gggggttgga tttcaaaatc ggtgtggacg gcggcgttga
    14881 ttttaagact gtggccgaat gcgcccgggc cggcgcggat acctttatca gcggaagcac
    14941 gctctttggc cggcccaacc tgaacgcggc agtgagaaaa atgcggaaag ccgcggaggc
    15001 ggcggtcggc caggcggggg tggcggcgcc cgcaggtccg gagccggccg gcgcgagaaa
    15061 ggcaaactga gcaggcggca gcagggcaat ttatgagcat caagtttgga accgacggtt
    15121 ggcgggcaat tattgcggac gagtttacct ttgcaaatgt cgcgcgcgtg gcgcaggcga
    15181 gcgccgatta ttggcgcgtc cggcgcgtgt ccggcacgga gaagaaggtg gtgatcggct
    15241 atgaccggcg ctttctgtcg gacgcctttg ccgcccgcag cgccgaggtt tttgccggca
    15301 atggctttca ggttgtttta acgccggccc cgacgcccac gccttccgtt tcttatgcgg
    15361 tcaaaagcca gcgggcgatt ggcggcgtga tgattaccgc cagccacaac ccgccgtcct
    15421 tcaacgggtt caaactcaag gctcattatg gcggatccgc cgcgccggag gagtgcgcgg
    15481 gagtcgaggc ttggctggat aagcggcccg tcaaggccac gcccctggcg gacgcgatga
    15541 aagccgggcg gatccggatt aaagatgttc gcccggctca ttatgccgcc atcaagcggc
    15601 tggtggactt taagcggatc gccaggtccg gattgcgctt tgcgcatgaa gcccttttcg
    15661 gtgtgggagc cggttgtttc gaggagttgc tggcggggac ttcctgccgc gtgaccacgt
    15721 tgaacgcgcg ccacgacgcc ttctttggcg gcatcaaccc ggagcccatc gaggcgaact
    15781 acgcgcgcag cgccgctttt ctcaagcagc atccacatga tatctgcctc gtcaccgatg
    15841 gcgacgcgga ccgggtgggc ggcatggatg gccggggcaa ttacctgacc acgcatcagg
    15901 tcatctgcct gttgctgcat cacttgattg tcaaccggca actccggggg cgggtggtta
    15961 aagcgcttaa caccacctcg atgatggatg ccatatgcgc ggcttacggg cttgagctgg
    16021 tcgagacggg ggtcggcttc aagtatattg cggccgaaat gataaaaggg ggagtcttgc
    16081 tcggtgcgga agaaagcggc ggcatcggat ttcccggcca tatcccggaa cgtgatggca
    16141 ttgccgccgg gctcgtgctg ctggagttgc tggcgaccga gcgcgtctcc gtccataaga
    16201 ttctgggctc gctggtcaaa aaattcggcc ctcacgagta tgggcgcata gacacgcggt
    16261 tcccgctgga gaagcgcgcg gagctgatgg actttttgaa gcggaacccg ccggatcggc
    16321 tgctgcgttc gggagtcgtc gaggtaaaga cctatgacgg ggtgaaattc atcgcgcggg
    16381 atgcctcgtg gctcatggtg cggggttcgg gtactgaacc gatcctgcgt atctatgcgg
    16441 aagcgggctc cgccgccgca gtgcggaaat tgttgcaact gggagtgcga ttgactcgtc
    16501 agatctgagg ctgctgcgga ggtgaacgtt ggggttccgg ccgcgccgcg tagtggggag
    16561 ccgggaccgc ggaatggcgt tgagaccgtc gaaggccggt cattgggtga tcaggcgggt
    16621 gcggcccgtc tggccagcgc tgcttgacgg cggttgaatc caggctgcaa aggcagtgcc
    16681 gggcggggcc aggagcggag catgccacgc aaggaatcag tactcctgat tatccggtga
    16741 gacggagctt tccagcgaat ggcagaactg gacgatgcgc gcagggggaa gtttcaacca
    16801 actcccaggg ccgccgtcaa ggccgggaaa aaccgcaacg cgcagccggg agaaaggcgt
    16861 tgctgcggga agaaagccag gaagcggttg gcggctggcg gtgttgttta tgattggcaa
    16921 gctaacgctg acctttgata cttacgcggt tgaaaactat ggccggcgat aagaagcacg
    16981 tttatgacga gagcaagatt aagacgctct cttcgctgga gcacatccgg ttgcgcaccg
    17041 gcatgtacat tggccgcgtc ggggatggtt cgcattacga tgacgggtgc tacatcctgc
    17101 tcaaagaggt gattgataac gccattgacg agttcatcat gggctacggc aaggaggtgc
    17161 agatcaccat ccaggaccgg gcggtgtccg tgcgggattt cgggcggggc attccactgg
    17221 gcaaggtggt ggattgtgtt tccaagatca atacgggggc aaaatataac gatgacgtct
    17281 ttcagttcag cgtcgggttg aacggcgtcg gcaccaaggc ggtcaacgcg ctgtcgaagg
    17341 tgtttcgggt gtgcagctat cgcgaggggc agtttgccga agcctcgttc aagcagggca
    17401 agctcaagaa ggagaatagc ggcaaggcgc cccgggaggc ggacgggacg ctgatcgagt
    17461 ttgagcccga tccggaggtg ttcaaggagg tcgagttccg ccccgagcac gtggagcggc
    17521 ggctgcggca ttacagctac ctgaactcgg ggctcaagct gcggtataac ggcaagacgt
    17581 ttcaatcgcg caacgggttg ttcgacctgg tcatggagga cctgcaggcc gatggcagcg
    17641 agccgatcta tccgccgctg cattatgtgg agaagacgct ggaattctgt ttcacgcaca
    17701 gcaacagccg ctatggcgag acgttctatt cgtttgtgaa tgggcaatac accagcgatg
    17761 gcggcacgca tctgagcgcc ttccgcgagg ggctgctcaa ggcggtcaac gaatacgcgc
    17821 aggagaagtt tgacggcgac gacgtgcgcg agagcatggt gggggcggtg tccatccggc
    17881 tcaaggaccc ggtatttgaa tcgcagacca agaacaaact gggcaacacg gaaatccgca
    17941 cggagctggt gaaccgggtg cgcgaggagc tcctgcagtt cttcaaccgg aacaaggaaa
    18001 tcgccgggaa ggtggtcgcc aaggttcagg acacccgcca gctccgcaag gagctgcagg
    18061 cggtgaagaa gctggcgcgt gagcgcgcca aagccatcac tatccgcatt ccgcagctca
    18121 aagactgcaa ggagcattac aaccgggcca aaggcaaggg gaaggggacg atgatcttca
    18181 tcacggaagg ccagtctgcg gccggctcga ttataagctg ccgcgacgtg gagacgcagg
    18241 ccatcttcgt gctcaaaggc aagccgctca acgtctggga ccttaagcgg gatgtggtct
    18301 ataagaacga cgagctctac aacctcatga gcagcctgga tattgaagac cgcctcgagg
    18361 gcctgcggta tgagaaggtt atccttgcca cggatgccga tgtggacggc ttgcatattc
    18421 gcaacctgat gattacttac ttcttccgct tcttcgacca actcgtgcgc gacggccact
    18481 tgttcgtgct ggaaacaccg ctgttccgcg tgcgcaacaa ggagcaaacg ctctattgct
    18541 attccgaggg cgagcgggat gccgtggcgc agaagctggg cgcgggctgc gagattaccc
    18601 gcttcaaagg gctgggagaa atcagcccgg ctgagtttaa gcaattcatc ggcaaaaaca
    18661 tgcgcttgag ccaggtcgaa tatgcgccga agcacgacgc cgccggcatc ctgggctttt
    18721 acatgggcaa gaacacgccc gagcgcaaag actacatcat ggagaatctg gtcgtgccgg
    18781 cggaggagtg aggccggcgt gcctgcctgc ttatcgcgcg cgctagcggc gttttgaaac
    18841 tgccggtgga aggagccatg cctccgccgc ggaataacca tttctcgagt gctttggcgg
    18901 cggaagaagt ggttcaaggc gggagggcct ccgcataagc gtggcgcagc agattttaat
    18961 cgcgtggccg tgctggaaaa tgtattggtt atctactaga tgtccgctag acgcaagaac
    19021 aacgctgacc agccggagct gccgattcaa gtgcccccgg acaacacgcc gggcgtggtg
    19081 aacgccgcgc ccgtgccgga aggtggcgcc actgaagcgc cgcggcgcgg gtctgcccgg
    19141 aaaggcaatg aggccacgca tgtgctggcc gagaaagttg ccctgccggc tccggcgcgg
    19201 tcttttgtgc cgggcaagat cgagttgccg ctgcaccgcc gggttgatcg caacttcctc
    19261 gactacgctt cctacgtcat tcgggatcgg gccattccca atctcgcgga tggcctcaaa
    19321 ccggttcagc gccgcatcct ttgggccttg cacgacaagg atgacggacg cttcatcaag
    19381 gttgccaatg tggttggcca caccatgcaa tatcatccgc atggcgacgc atccatcggc
    19441 gacgcgctgg tggtgctggc caacaagcgc tacctgatcg agggccaggg caactttggc
    19501 aatctcctca ccggggacct ggccgccgcg ccgcgctata ttgagtgccg gctgacggaa
    19561 ctggcgcgca ccgagctgtt caacgacgag attaccgagt tggtgccgag ctatgatggc
    19621 cgcaaccagg agccggtagc cctgcccagc aagttgccgc tgctgctgat gctcggcgcc
    19681 gaaggcatcg ccgtaggcat gtcgtcgcgc attttgccgc acaattttcc cgagctgctc
    19741 gaggctcaaa tcgccatcct taagaagcag ccgttcaagt gcctgcccga ttttcagact
    19801 ggcggtctca tggacgcccg cggctacaag gacggcgcgg gggaaatcaa ggtgcgcgcc
    19861 cggcttaggg tcaaggatga gtccaccgtt gtcatcaagg agattccgcc caccaccacc
    19921 acggattcat taattggttc gatcgaagat gcttcgcgca aaggcaagat caaggtcagg
    19981 agcatcaacg atttcacctc cgaggaggtg gagatcgagg taaaggcgcc gagcggagtg
    20041 agtgcggagc agttggcgga cgcgctttat gcgttcaccg attgtgaagt caccatctcc
    20101 agccgcatcg tggtcatccg gaacaaccgg ccggtggagt tgaccgtgtc cgaggttctg
    20161 cgggagaaca cagcgcagtt ggtggagatt ctcaagcgcg agctggagct gaaagaaacc
    20221 catctgcaag atgagctgca cttccgcacg ctggagcgca tcttcatcga ggaacgcctc
    20281 tacaagaaga ttgagcagtg ccggaccaac gaggcggttt tcacggccgt gcgcgagggg
    20341 tttaaaccgt ttcgtcgcca gttgattcgc gagctgacgg agcccgacat cgagcggctt
    20401 ctcaccattc gcatccgccg catttcgctt ttcgacatta acaagcaccg cgaggaagtc
    20461 gagagggtga agattgaatt ggccgaggcg cgcaagaatc tgaagcatct cacccggtat
    20521 gtcatcggtc atctcgaagc attgctggcc aaatatcggc cgctttatcc gcgactcacc
    20581 cggtccagcc gttacgacga agtagacgcc aaagaagtcg ccttcaaggc attcaaggtc
    20641 gcctacgacc gcgcatccgg ctatgtcggc cacaaggttt cgggttcgga atttcaggtt
    20701 gagtgcactc ggttcgacaa gttgattttc gtattcaagg atggccacta tcaggtctcc
    20761 gaactgccgg agaagcttta tgtcgggcca gacctgatct attgcgggtt gccggagcgc
    20821 gaccgggtgt tcacgtgcgc ctacaccact cgcgaggcca cctacctgaa gcgcttcact
    20881 tttggcggcg cgatccttaa caaggtgtat cgttgcattc cccccaaggc gcgcgtgctg
    20941 tttttcgagc ccgatacgcc ctctgagctt tatatccggt ataagccagc gccgtaccag
    21001 aagattaatc agcaaacctg ccggcccgct gaggtggaag tgaggggtcc aaagacgcgg
    21061 ggccggcaag tctccatcaa ggacgtatcg tccattacca gccagcccac gcgtggatgg
    21121 gatccggagg cgccgactac ccggttgcag tttgcgtagg ggtgacttgt attgggtcgg
    21181 ggcgttcccg atgagcgtga gggaagggag gaagcgtttg ggagattctg cccgtgcacg
    21241 gttaggaggt taggtcttga tttcggcagg cgtaattgta aggtggagag ggtccgtggt
    21301 ataatcgcaa ggctatgaaa ttatcactat tgtttggacg aggattgcca ggcgtggcga
    21361 tcgtcctttt gagtgccctg ttgttcaccg gctgcgccac cgtccccgaa accgggcgca
    21421 gccaattgaa cctgatttcg gccgcggagg aaatgaaatt agggctcacg gaatttgaaa
    21481 agaccaagca gagcatcccg attagcaaag atgccaaggc cgccgcgatg gttcaacgcg
    21541 tgggacagcg catagcggcg gttgccaatt tgcccaatgc tcagtgggag tttgtgctgt
    21601 tcgacagtgc ggaggccaac gcctggtgcc tgcccggtgg caaagtgggc gtttattccg
    21661 gcatcctgcc gataaccaaa gacgaggggg gcttggcgac ggtcatcggc cacgaaattg
    21721 ctcacgccac ggcgcatcac ggtgcggagc ggatgagcca gctaatggcc gcccagctag
    21781 gagagagtgc tgtgggtgcg ctaacatccg gcagcagcca gttgacgcag gctttggttg
    21841 gccaggctta cggtcttgca agccaggttg gggtgctgct gccttacagc cgcaaacaag
    21901 aggcagaggc agattatatc gggttgctct atatggcccg tgccggctat gacccgatgg
    21961 cggcggtagc cttctgggaa cgattttcgg cgtataacga gcagcacggg ggcaaccggg
    22021 agttttcctt tttgcgcacg catccgttgg accagcagcg cattcaaaat ctaaaagcca
    22081 aaatgcccca agcccaggcc gaataccagc gggcgaaagc agccggcaaa taagaacgtt
    22141 cttaagcgcg ggccggaggg atatctttgg tcagagcgag gatgccggta acaatattgc
    22201 tgggaacttg ctcgaagcga aacggctcca tggaataggc ggcgcggcct tttgacagcg
    22261 agcgaatcgc ggtggcataa ccaaacatct cggccagcgg gacttcggcg gtggcgatcg
    22321 ttgaggtgtc tttggcctcg atttgcagga ttttaccccg gcgccggttg aggtcgccca
    22381 gcaggtcccc ctggtattca tccggcgtgg tcagttccac tttcatgatg ggctccaaca
    22441 ggatgggatt ggctttttgg gccgcttctt taaaggcaaa aatgccggcc atcttgaagg
    22501 ctaactcgct ggaatccact tcatggaacg cgccgtccac aatggctact ttcacatcca
    22561 ccacagcgta accggccaga actccgcctg cgatagcctc cttgatgccg tctatcacag
    22621 ccgggatgta ttccttggga atcgcgcccc ccacgatctt gttctcgatc tcaatgcctt
    22681 tgccgcgctc gttgggggcc agcgtgatgc aggcgtgccc gtattggccc cgccccccgg
    22741 actggcgaat aaatttaccc tcgccttcag cagccttggt gacggtttcc cggtaagcaa
    22801 tctgcggcgc gccgacattg gcgctgactt taaattcacg gaagagacga ttgcggataa
    22861 tgtccagatg caattcgccc atgccggcaa tgatgagctg cccggtttct tcattggtaa
    22921 agcagcggaa ggtggggtct tcctcagcca ggcgctgcaa gccttcgctc atcttctcgc
    22981 ggtccgcctt ggttttgggc tcgacggcca tcgagatgac aggctccggg aaggtgggcg
    23041 gctccaggag cgcctcgaag tcctcatcgc atagggtgtc gcccgtggtg atattcttca
    23101 cgcccactag cgcggcgata tccccggcaa aaacagtctc cacgtcaatc cgcttgtcgg
    23161 cttgaatcat cagcacccgg ctgacccgtt cgcgtttgcg ggtccgtgga ttgtagatga
    23221 cgtcgccctt gctcaagcgc ccgctataca cccggaaaaa caccagcttg cccacataag
    23281 ggtccgtcca cagtttaaac gccagcgagc agaacttccc attgtcgtcg gagtaaactt
    23341 cgaccttggc ttccgtgtcc ggcgtgatgc cgacggctgg agggatatcg agcggggacg
    23401 gcagatagtc cacaatcgcg tcaatgagcg cctgcacgcc gcgttttcta aaagcggagc
    23461 cgcacagcac cggcaccagc tcgagtttgc aggtcaggcg ccggatggcg gctttcaggg
    23521 cttccggcgt aaccggctgg ttttcgatca ccagttcggc gatccggtcg tccttgttgg
    23581 aaacggcgtc aatcaattcc gccagcgcca ttgcggccgc ttctttaagt gccgccggga
    23641 tatccgtgac ctggcagttg aggccgtgct cgtcgttggg gtcgtagatg attgcctttt
    23701 ggtttacaag atcaaccacg ccgtcaaagt gatcctcctt gccgatggga agaaaaatgg
    23761 gataggcgta tgcgccgagt tttttgcgca tgtccgccag cgcattcatg aaattcgcgc
    23821 cgacgcggtc cattttgttg acgaaggcga tgcgcggaac tttgtacttg gtggcctgcc
    23881 gccagacggt ttccgattgc ggctggactc cggcgacgcc gcaaaacacc gccaccgcgc
    23941 cgtccagcac gcgcatggaa cgctcgacct cggccgtgaa atccacgtgc ccaggagtat
    24001 caatgatatt gatgcgatgg gggattccgg cgaacgcctt gtataggccg tcggctttct
    24061 gggtccagaa ggaggttgtc gccgccgaag taatggtaat gccccgttcg cgctcctggt
    24121 ccatccagtc ggtgaccgtg tttccgtcgt ccacatcccc cattttgtgg atgagcccgg
    24181 tgtagaaaag aatgcgctcg gtggtggtgg tcttgccggc atcaatatgc gccgcgatgc
    24241 caatattgcg ggtatgttcg atcggatact gccgctgggg cacaaccggg cccttggtct
    24301 tgggggatct cctggtttct gatgcggttt ccatgcaggg ggctttcgag aaaataaaaa
    24361 cgccccgggt caggtcggcc tgcggggcgt taatttaacg atgattacca gcggaaatgg
    24421 gcaaaagcct tgttggcttg cgccatcttg tgaacctcgt cgcgcttgcg aatcgcattc
    24481 ccctggcctt gataagcatc taaaatttcc gccgccaggg cttctttcat cggaatgccc
    24541 ttgcgcgcgt cagcaagatc caccaaccag cgcagcgcca acgagaactg gcggtcggtg
    24601 ggcacttcca acggcacctg ataggtggcc ccgcccattc gccgcgcctt gacttccagg
    24661 cgcggtttgg cgttatcaac cgcccgctgc aagacttcca gcgggttgat ggcggggttt
    24721 ttggcaacga gctcatccag agcgccatac acaatgcgtt gcgccacgct cttgttgccc
    24781 tgcttcatga cggtattgac caggcgcgtt accagcgcac tattaaactt ggcatccttg
    24841 gggatgggcc gactgattgc tcgacgacgt cgcgacatgt attcgttaaa acgtaattag
    24901 attgtgggtt cgggagttag ctgtttacgc ggctggcgcg gcggccgccg cggcggccgg
    24961 gcgggcgccg gctttgggcc gtttgacgcc atatttcgag cggctgaccc ggcgtttctc
    25021 cacgccgacc gcatccaggg tcccgcgcac gatgtgatac cgcaccccgg gcagatcctt
    25081 cacgcgcccg ccccgtatca ggacaatcga gtgctcctgc aagttgtggc cttcgtccgg
    25141 aatgtaggcg atgacctcgt atccgttagt caggcgcact ttggcaacct tgcgcatcgc
    25201 cgaattaggc ttcttgggcg tgcgcgtcat tacttgaata caaactcccc ggcgaaaagg
    25261 cgagttttcc aaggcgggag acttggactt atgctttagt ctgctgcggc ccttgcggac
    25321 caattgatta attgtcggca ttcggtttaa aattcctcgc ccgcttccag tgcggcaacg
    25381 caaccagagt ttcaggcaaa cagaagctga aatatagcaa acaggctttg gagcgcaaca
    25441 actatattta gcaacttatt acgtcgcttt gcctcaaaat aaacggcagg gtcaaccgcc
    25501 gggagtctcc tggcggttgc tggggggaca ggagccccgg gcgcgcatgc cggaccccat
    25561 agtcccaatg atgctttagc gcgagggtct tcatttaagc gccgcgcgtt gaggcaaccg
    25621 gttcgttttc cgcgtccggc gcgctccctt ctttgatgga ggcaagccgg cactcgtcca
    25681 ccatgctcga ttctggccgc cgccaaagtt atttatcgtt ggcggccggg agccgaaccg
    25741 ggtccgcctc gtcacccatg aataatttgg gagcacctat aagcgggcgg ccaggagcaa
    25801 cccggccaga gctaagcacg cccaaccgaa gcggtcgccg tattgacggt agggggttgg
    25861 cgggtggggt tgatcgggag tcagcagcgg gatgtcggcg atcaggaatc ccgggccgta
    25921 gatggtgccc cgggcgtcgt agaagacctg gcgcaggcgc ccgtgggcat ccacccagcc
    25981 ggtgaggcca ttattggagc agcggatgag aggccggtgg ttttcgatgg cccggaacac
    26041 cgcggtggcg gcgtgctgcc attgggcggc gctttcgcca aaccagccgt tgttggtgag
    26101 gttaaccaga aagtcggtgt cttcgtcaac cgcggtgcgg gccaggccag gaaaggtgtc
    26161 ctcgaagcaa atcagcgtgg aggtttttac tttgggcgtc gtcagcgcaa aggggaccgc
    26221 acggtcgccg ggggtaaagc cgctgccgat gggagtgaac cacttgagaa acggcaacca
    26281 gcgggccagg gggatgtatt cgccaaagat gaccaggttg cgtttgatat aacgggcaac
    26341 aaattcgcct tcggggctta agaggaaact ggcgttgtag aactccagct cgttgtcgcg
    26401 cgaggggcgc gttgaggggg cggcgtcgtc ggagccgatg atgagccaga catggtgttg
    26461 gcggacgagg ttggtgaggg ccgtgtaaat atcagcatcc cattgggcca ggttggggat
    26521 ggcggattcc ggccagataa gcaggtctgt gcggttggtt agggccagcc tggatagctg
    26581 gaggacctcc tgaaaacgct cccaatcgcg gctggagtcc cagatgaggg tttgaggaat
    26641 actgggttga atgagggtaa ctttcagaga acggagggga ggcggttcaa aggcaagctg
    26701 gcgaaatcca aaatgcaggg ctgccgcaat cgcggctgcc ggaagcagga tttcaccagc
    26761 ccacgttgat cgggaggtgg gacgcgatac caccatcagg ccggccgaca gcagtgagag
    26821 cgaggtccag acgacgagga acgaaacccc atagacgccg gcaatcgagg cgacctggat
    26881 gagcgggatt agctggtatt gcgaggcccc cagcgggttc cagggaaagc cggtgaaaag
    26941 gcgggcccga atcatttcga gcgcgaccca gagggcagcg cccccgatcg cccagagagt
    27001 gcgccgcccc caactgcaag ccagcacgtt caccgccccc cccggccacg ggagtgttcc
    27061 cgcaagccgt ggttcgggcc gtttgcgcaa gaagccgaag cgggcggagg tattcgtgtt
    27121 ccgggacagg ctcatggtca gcagccagac ccatgccgcc ggatagaggg cgacatacgc
    27181 actgagggat acccagccaa gcgcggggcc gagcggaatg gagtgccagc ggtaagggat
    27241 cagcagcagc cagtaaagcg aggcgagata gtaggcgagg ccgcccaggt agccgatgcg
    27301 aaaactctcc caaccgcgct tgcccaaggc ggcccccaac agcagggcgg gagctaccca
    27361 ggccaggccg gcaaggccgg gtttgggaaa ggaagccgcc agcagaagcc cggcaaggac
    27421 agccagcagg caacgactgc gcaaccaaga gttgggccta aagaagctca cctgacccag
    27481 cttgcccatg aagagttgaa acgcaagatc aggatggcca gcatcgttaa ccccggcagg
    27541 ggcttttatt atcggcgtct aagccggaac gattcagttg aaaaagtaag caccgtttgg
    27601 cggacttggg ag
//
LOCUS       DAOPQG010000043        27586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2226_length_27586_cov_18.175366, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000043 DAOPQG010000000
VERSION     DAOPQG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 27586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27586
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2226_length_27586_cov_18.175366"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..348
                     /locus_tag="PKI07_06680"
     tRNA            272..348
                     /locus_tag="PKI07_06680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:306..308,aa:Arg,seq:acg)
     gene            complement(1325..2527)
                     /locus_tag="PKI07_06685"
     CDS             complement(1325..2527)
                     /locus_tag="PKI07_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOC50777.1"
                     /translation="MNPQVPLSELTGRMQAFRARMDAAQPDWEMAAFLGKVNQYYLTG
                     TRQDGVLLVPRDGEAVFWVRRSFERAAAESCFPNLRPMKSFRDAAPAGAAAAPRRRIH
                     LETGLVPFALLQRFLKYFPCREVAAVDPHIAGVRAVKRPYELALLERAGALHRRVLED
                     AAPALLRAGMTEAELACELYALMVREGHDGTVGFSQFNVDIAVAQLGFGENSLYPTSF
                     DGPGGCRGLNSAAPVLGSRERKLQKGDWVFVDLGCGVEGYATDKTMTYMFGAPPPEAA
                     LEAHRQCVELQLRLAALLKPGAIPSAIYARTLAELDPRFQVNFMGCGNRRSAFLGHGV
                     GLQIDEPPVIAEGFDEPLAEGMVLALEPKKGVPGVGMVGTENTYLVTPAGGRSLTGSH
                     PGPILVSS"
     gene            2709..4295
                     /locus_tag="PKI07_06690"
     CDS             2709..4295
                     /locus_tag="PKI07_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50778.1"
                     /translation="MQTSFRRSVRLAPGRARAGGLLAAAALFIAAPPAPRAAAPPETA
                     APANFRAGFAERDITPEIGMEAPGNYGKNYHRSFHDPCKVRAAVFDDGRQRVALVGVD
                     LLFITRHLATGARAEIQQRCGIRPEAVLIGASHSHSSGPIGMSEPGDYDAAPPLVKRL
                     ATEHSIVSNPGYTQWLRRQIVEAVADADARRGEARLAVGFGREEQVAFNRRFRMKNGL
                     TFTHPGAGNPEILGPAGPIDPQVGVVGVWDLKGGLLGCVVNYACHATTSPGGISANWI
                     GAMEQTLRRASGHAALPVVFLQGASGDVTQVDNLTKVQNPQAEEWCQLVGGRIGAEAY
                     KTLLLIQRGASSHIPLDVRQKIWRIPRRAPAPEKVARARELAQKPAREVGEPAWTFAK
                     ETVLLDALILARPEAEVEVQALQVGPLVLVANPAEYFVQYGLDLKQGSRFPFTFPVEL
                     ANGCVGYVPTEEAFSAQGGGYETRLTSYSNLHVSAGRQFLETGLELANQMTPGAVPLP
                     PPAPPFSAPWSYGNVPPELE"
     gene            4485..5420
                     /locus_tag="PKI07_06695"
     CDS             4485..5420
                     /locus_tag="PKI07_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50779.1"
                     /translation="MKLLDSPRSGRLGAYVYYSTPFGQCCRALTMPRDARSPAQARAR
                     ASFAACSQAWGRSLTEAQRQRWVAAAQTVPSRPSVGQYGPLSGQQFYVRINSVLDCIG
                     QPPVSEPPAPVVFSPNLVTGLEIVQDPEAGLRLRLNVGVATEDIMVFGQAPCSPGRMK
                     HRRVYYLGLLSPSQNGQSDITELYTARFGQPAPGQKVFIVTSQTRNGWKGPNWVHSAI
                     VPLPSTAKSQQATEKAPVKKAKPASTLPSSTARTTVSSSSSQAMYKGSTPEARQERME
                     NKHEPPLSILGTPLVHGLLGALKWLGRMGISRATA"
     gene            complement(5503..8769)
                     /locus_tag="PKI07_06700"
     CDS             complement(5503..8769)
                     /locus_tag="PKI07_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC50780.1"
                     /translation="MSTPSSSTDAPHLGFAGRIARAFIDSKLTPLLVITSILLGAAAT
                     WLLPREEEPQIKVPMIDVLVAMPGFSAKEVEERATRPMEKLLWEIPGVEYIYSTSREG
                     ECLAIVRFKVGEDITRSLVHLNQKLQANFDRIPFGVSYPLIKPRSIDDVPILALTFHG
                     AGYDHYTLRRVVAEVDDQVKQVPLVAETMLIGGERRQLRVLLDPARLAAHNLSAAEIL
                     PRLQAANRQLAAGSLISQNREILLQTGNFLASAQDAGGVVVGVPGGQPVYLRDVAEIV
                     DGPEEPSQYVFSGAGAARAAPAGEEPAVTLTIAKRPGANAIAVARAVLEKVAALQGRV
                     IPHGVEMTITRHYGQTAEEKSDELLLHMGIAVVGVSLLILLTLGWRESLIVGIAIPST
                     LALTLLIFFLYGYTLNRITLFALIFSIGILVDDAIVVVENIVRHFHLPHNKGRPWSVI
                     AVEAVSEVGNPTILATFAVIAAVLPMAFVGGLMGPYMRPIPIGSSAAMFWSLAIAFMV
                     TPWAAIRILRWGRKYARVTEAAAGAAPAGQPHSPFEHEEDFFTRLYRRFMDPLIARAR
                     WRLVFLAGITLLLLGAMALVGLGWVKVKMLPFDNKSEFQIILNMPEGSALELTAQAAR
                     EIAAAVRVEPEVTDYQVYAGVAAPFNFNGLVRHYFMRRGPHIADLQINLVAKDQRRAQ
                     SHEIAKRVRPRVRDIARKYGARVAVAEVPPGPPVLQTLVAEIYGPSEENRLALARKVI
                     EIFHHTRGVVDTDWHIEADQAKARFLVDKEKAALHGISEESIAQTLKLAVGGAEVDLL
                     HLPREKEDLIIQARLPQSLRSSPEDLLALRVRGASPDGALVPLRELVTVETVAADKSI
                     YHKNLMPVTYVIGDVAGEVESPVYAIQQMNKALAQIDTRPFGGTGTPLRIYNAHQPFT
                     DSEPALKWDGEWHITIEVFRDLGAAFAAVLVLIFILMVGWFRSFVTPLVVMMAIPFSL
                     VGILPAHGLMGAFFTATSMIGFMAGAGIVVRNSIILVDFIELRLAEGMPLAEAVVDAG
                     AVRFRPMLLTAMAVVVGASVILADPIFQGLAISLMAGEIASLLMSRMAVPVLYYMVRR
                     "
     gene            complement(8779..9828)
                     /locus_tag="PKI07_06705"
     CDS             complement(8779..9828)
                     /locus_tag="PKI07_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC50781.1"
                     /translation="MKAIPTYALSFRSGLLLAGLAALLNGCGEKPAPPAGAGPSLPPA
                     AVRLQKIEAVSQPALEEVVGTVLPRLQAVLEAKVSGRITRLSAALGQSVKQGDVLVEL
                     ATLEIQARLDQARAALRQAELDLNRTTRLHKQSAATQAELDAAQTRHQVAQAAVAEAE
                     ALAGYGRIAAPFDGVVARKLADEGDLAMPGKPLLELEGRAGLRLVADVPGLLAPRIRP
                     AAELAVRVDAIPEPLTGTVAEIAPAADPASRTVRVKVDLPAAPGLRAGQFGRLAVPVS
                     ETTVLLVPPAARVRRGQLEILFVAADGKARMRLVRSGKQTPQGLEILSGLAPGETVVV
                     DGAATLREGQPLSTP"
     gene            complement(9825..11153)
                     /locus_tag="PKI07_06710"
     CDS             complement(9825..11153)
                     /locus_tag="PKI07_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC50782.1"
                     /translation="MTKAVAICGLALLGAWAALAAEPWTLERALRQALADNPDARLAQ
                     HRLAAAQAGLDQANAAFWPRLQFQSSYAGSDNPMQAFGSILNQRAYNYGSPPDFNDLP
                     AVDNLNVRGLATVPLYAGGRTTAARHAARAHTDAARQDEAALRNALGFEVTRAFHTVL
                     KTRQFVRAARAAREALENSLAVARRRHDSGTLLKSGVLDLEVRLAQAREDLVRARNAE
                     ALATRALRALLGVEPEDPDEFEVADTAPALEAPDAGDFSGRPELAASRHRERAAAEQV
                     REARSGYLPRISAFGSLDYDHGWKFNHGGGSWTAGALLQWDLWDGQLTRAKVREASAQ
                     LEAARAEQRKLRLALDLETAQARLDLQAAAERRAVTEHAVAQAQESATLTRARFEQEL
                     ALPRDLMDAETALITARVRRAEAEADWQIAIGALRKALGLPQLNFETPAK"
     gene            complement(11150..11380)
                     /locus_tag="PKI07_06715"
     CDS             complement(11150..11380)
                     /locus_tag="PKI07_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOC50783.1"
                     /translation="MKMHMIIRRFAGSFILLSLLLAHYHSPYWLWFTAFVGFNLLQSS
                     FTGFCPLEVILRKANVGRDDSSAPAPRACCSK"
     gene            complement(11408..11575)
                     /locus_tag="PKI07_06720"
     CDS             complement(11408..11575)
                     /locus_tag="PKI07_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOC50784.1"
                     /translation="MILHIIIGAIAGGGLGFALYKFVGCSTGACPLTSNPLISTLYGG
                     LVGALIASSIR"
     gene            11732..12217
                     /locus_tag="PKI07_06725"
     CDS             11732..12217
                     /locus_tag="PKI07_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOC50785.1"
                     /translation="MKLTAVRIEKPEPINFILGQTHFIKSVEDIHEALVGAVPGIKFG
                     LAFCEASGKCLVRESGTDPALVELARRNALAIAAGHSFIVFLGEGFYPINVLNTLKAV
                     PEVCRIFCATANPTQVLIAATEQGRGIIGVVDGSPPRGVEAEPDIAWRKNFLRQIGYK
                     L"
     gene            complement(12254..14605)
                     /locus_tag="PKI07_06730"
     CDS             complement(12254..14605)
                     /locus_tag="PKI07_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC50786.1"
                     /translation="MTSLSSLRFRLVGTVFLAVAPASVLLYFGNKYYASHYGAQLPWT
                     EFLVGLLALGAAWFGGERFIIRQVRVLSLAARKLGAGDLTSRTGLSRERGELGELSRT
                     FDSMAAQLEEREKESERVKRTLTHRTMQQTAVAALGRFALETSDPSILLNHAATLAAQ
                     TLDVEFCHVLEVQPGGKFLLMRAGVGWKDGEVGKVVIPADPQTETGFALTAKEAVAFA
                     SLGNEGRFRGSSLLTNHKVVSGVTVAIAGQEQAFGVLGAYTKRPRTFTEDEVHFLFSI
                     ATVLAMAVERIRVETELQKLAAFAQLNPNPALELTSDGAITYFNNAALKLSRSVGRDH
                     PRELLPDNVQEIVQNCLKAGRSVLDLSTVLEKRSLSWSFHPVAASHIVHCYVEDITDR
                     LNLEAQLRQSQKMESIGQLAAGVAHDFNNMLTVIQGHSGMVMANTDLAPALLDSAQAI
                     YFAAERAATLTRQLLLFSRKNVMQPKALDLRNVVGDLSKMLKRLLGETVALQFNPPAE
                     LPLVAADAGMLEQVIVNLAVNARDAMPKGGTLTIRTTPVMLNEAFVQMHPQARPGAFV
                     CLEVNDTGCGMDMATMARIFEPFFTTKEVGKGTGLGLATVYGIVKQHEGWVEVSSEVG
                     KGTTFIVYLPASDEPAPARAARTTEAAAVRGGHETILVVEDEIVLRDLAQLILHDCGY
                     RVLVASSGAEALQAWERQAGGIDLVVTDMVMPGGMSGLELAAKLKAIQPQLKLIFTSG
                     YNMEKSDTDFFRRHGALFLQKPYTRPDLANVVRECLDNPAREG"
     gene            15066..15413
                     /gene="yajC"
                     /locus_tag="PKI07_06735"
     CDS             15066..15413
                     /gene="yajC"
                     /locus_tag="PKI07_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOC50787.1"
                     /translation="MSLFSPNALLALAPPAQQPGQSAPPAWVNMVPLVLLVVVFYFVL
                     IRPQQKKAKDHATLLKTIRPGDKIVTSSGIVAVVVTVKEKTLSIRSADAKFEITKSAV
                     AEITERGNDSRES"
     gene            15423..17912
                     /gene="secD"
                     /locus_tag="PKI07_06740"
     CDS             15423..17912
                     /gene="secD"
                     /locus_tag="PKI07_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOC50788.1"
                     /translation="MNRNYFWRTVLVVLIVLWSLYELYPPKGRDLVQYFRDQAVNRDP
                     DFTTIFTNALALQKAMPEKPYENLLKAIDTNDITRYFPSFGAKEQLHPTPYILNRLQR
                     QAAGRIRLGLDLQGGTSFLVSMDTNQLAQAADAENALSHAVEVLRKRVDKFGVAEPLI
                     QPQGNNRILVQLPGLSAADQESAKAAIQRAAFLEFRLVCPTSEQDIKEGIVQPGYEIL
                     KRKQRLRDGREHIEEVEVRKRAEMDGSGLKSAMVVRGNLGEPEIHFTLDSKGAERFAE
                     ITRENVGQRLAIILDKELYSAPVIQSPIETGSGQITGQFDNREAFELANVLENPLRAP
                     LHIDESREVDPTLGKDSIRSGINAAIYGTLAVAVFMICYYLAAGVVANLALIANIIIL
                     LGVMCSIGTTLTLPGIAGIVLTIGMAVDANVLIYERIREESAKGKSLRGAIAAGYSRA
                     FTTIFDSNLTTLISSVILIYMGTGPIKGFGVTLTIGIMASLFTALVVTRLIFDFLADR
                     GWLKSLPMLHLIRAEKIDFLKLAPYTFAITWIIIVVGVIFTIRRGEAAFGAEFRGGDT
                     VLMAFEQKPGEAAVRTALTKAGIKDPRIQFQKDVTTRRETLRIDSSPGTAELVKSTLS
                     QMPAAKFHLLGQDRIGATIGKEIQKSAAIALLLSLFGILIYVAIRYEFSFAVAAVISL
                     IHDVLITLGLYSLSGREFNSATLAAILTIIGFSINDTIVIFDRIREDLKLGIRGSFRE
                     VMNKALNQTLSRTVITSGTVFLSTSSLYVFGGGAINDFAFTFLIGIVVGTYSSIYIAS
                     SLVLWWHRGQRPSIGAGAQAVMQNATAAALK"
     gene            17962..18927
                     /locus_tag="PKI07_06745"
     CDS             17962..18927
                     /locus_tag="PKI07_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOC50789.1"
                     /translation="MIGLINITAWHWAGFIGCVLVFLALDLGLFHRRAHVVKFREALL
                     WTSTWFCLAMLFALALKPLRGKEEALEFLTGYLIELSLSMDNVFVIAMLFAYFQIPRQ
                     YQHRVLFWGIVGALVMRGLMIGAGVALVAWLHWVLYVLGAFVLFTGLKMLFVETRVHP
                     EKNRVVKWVRKLYPVTPHLDGQKFFSTWQGRLALTPLALVLVMVETTDLIFALDSIPA
                     IFAVTTRPFIIFTSNVFAVLGLRSLYFVLAGALEYFRYLKVGLALVLAFIGCKMLLDP
                     HGGPPLWFQLKIPISVSLFVVGGIIVASIALSLTLGRQQPPRSAS"
     gene            18924..20666
                     /gene="recJ"
                     /locus_tag="PKI07_06750"
     CDS             18924..20666
                     /gene="recJ"
                     /locus_tag="PKI07_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOC50790.1"
                     /translation="MKFRWSLAPPQRAHAERLAEALKLPPLLAQCLLNRNLTEPSSAA
                     AFLRPRLRQLADPFLLPHMPLAVERLLQARERKEPVVLFGDYDVDGVTASALLLEVLR
                     PLGWQVQYYLPRRLEEGYGLSQEAVENCLAQSGATLLLAVDCGSTAVAPIAWLRQRGV
                     EVIVLDHHQISVPAPAAIALVNPRLSASCNTSESEDSGAPFTELCSVGLAFKLAHALL
                     KRARETSILGAADFDLRSLLDLVALGTIADVVPLTGENRILASVGLERLSTTQRPGLV
                     ALKQVAQSPALLGSHEVAFQLAPRLNAAGRLETAEEALRLLLTRDHAEARTLAQTLDV
                     RNRTRQKIERGIVEDAIATVQNRFNPSTDYVIVEGNPTWHIGVVGIVAARVLQQFYRP
                     TVILGSDGATYRGSGRSIPGFNLAGALRECADLLLRQGGHAMAAGVSLLPANLEAFRA
                     RLNQLALNTLQPEELQPSLRLDAEVRLVDLHWECLAALAQLRPTGQNNPPVQLFARKL
                     NLHRPPLHIGAQKQHLKLWINDGTTALEAVWWNAADQTIPPAPFDLAFAPQINEYNGS
                     RSVQLKILDWRPAQ"
     gene            20885..21574
                     /gene="ispD"
                     /locus_tag="PKI07_06755"
     CDS             20885..21574
                     /gene="ispD"
                     /locus_tag="PKI07_06755"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOC50791.1"
                     /translation="MVSAIIVAAGQSSRMGAGVDKLFLKLNGCPIVAHTWRQFELAEC
                     IDEIVLVVRETMRPAFAALGEEYRFHKPFRLVSGGKERQDSVWNGLQALSPHVEIAAI
                     QDAARPCTSSALIAATIAAARQTGAAVAAQAITDTIKESSDGKLIERTLDRSRLWSVQ
                     TPQTFRVEIIRQALAAVRRQGLLVTDDAAACELIGQPVQLVACLQPNPKVTRPEDLPC
                     LEALLRAPALP"
     gene            complement(21730..23073)
                     /locus_tag="PKI07_06760"
     CDS             complement(21730..23073)
                     /locus_tag="PKI07_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50792.1"
                     /translation="MKTTAQQNQTRFTSRRGFLKTSATVAGTALIGAIDVGRFAHAAG
                     SDTLKLGLIGCGGRGTGAAGNALTVDAGTKLWALADIFPDKIDTTINTLSPKFPDRIE
                     VPRGRQFSGLQGYRGVIENCDVVLVVCASRFHPDYTLAAVQNKKHVFVEKPAAVDIAG
                     VNKIMQADVLSKQNGTGVLAGVTYRYHLGRREAVQRIQAGELGEIVAVQCEYLRSPYR
                     LIEREPGWSEMEYQLRNWYHFSWLSGDDILQSLIHDLDSALWVLGDVLPSQAYGMGGR
                     STHFEATMGTCFDHNQVIYEYPNGRRIYGACRTAVGCYGSNQDIFHGTKGRCNFTAFQ
                     PPYFTDLNGNRTWRANVEQSAKSPYELEHSEFLQSIRAGKPFQRAKILADSTLVSVLG
                     QLAVYTGKKISLQEVLDSKFAFPPIGEITMNTEPPVKPGPDGLYPVAIPGQTKLL"
     gene            23309..24652
                     /locus_tag="PKI07_06765"
     CDS             23309..24652
                     /locus_tag="PKI07_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOC50793.1"
                     /translation="MKQHLLFGVLVTALSINLVIGARVYLNSAQAAEKDSAYPSLELF
                     SYALEKVRKDYVDGQNLTYRQLVYASLKGMLSTLDPHSEFMEPAKYKELQSDTQGEFG
                     GLGIVIAMRDGFITVIAPMEDSPGFRAGILSGDRIIKIGERSTEKMSLQEAVTNLRGE
                     AGTEVKISVLRPSSGKIKDYTLTRAVIKVDMVKDINGKKEFLLDDNKIGYIRLVQFGE
                     KTGAELEGAIKKLKAQGLQGLVLDLRWNPGGLLEQAVDVCERFLPRGNLVVTTEGRNP
                     TQNSVRKARGRGDELSGLPMVVLVNLGSASASEIVAGCLQDSKRAIILGEKTFGKGSV
                     QSIIPLAAMSDGVPALRLTTAKYYTPSHKVIHEEGITPDIIVPLSEEQERFILMKHAP
                     GGIQGMEESEREQVLRSGDPQLERAMDLLKGITLFTQRSPAFNQRLAKSKTRDAK"
     gene            24694..25719
                     /gene="tsaD"
                     /locus_tag="PKI07_06770"
     CDS             24694..25719
                     /gene="tsaD"
                     /locus_tag="PKI07_06770"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOC50794.1"
                     /translation="MPLLALETSCDETSAAVVDQGRVLAHLVSSQVQLHAEYGGVVPE
                     LAAREHLRHLLPVTRLALQKAGVDPEQLDAVAATRGPGLPSALMTGFKAAQAAAFALR
                     RPFLGIHHHEAHLYSPWINGPPPKADFSRFQPNISLIVSGGHTLLVHVRSELNHHLIG
                     STVDDAAGECFDKTGKLMGLPYPAGPEIDRLAQDGNPEAFVFPRPMIDEPNDDFSFSG
                     LKTSVRYFLRDHPKLLQDTQCLRDLCASIQAAIVDVLVTKTLRAARRLCVGCITVSGG
                     VASNRALRQRLGAACERQRFALMLADKAFCTDNAAMIGILAERKLRLHSEPTPLDADI
                     LPSWALE"
     gene            complement(25756..27471)
                     /locus_tag="PKI07_06775"
     CDS             complement(25756..27471)
                     /locus_tag="PKI07_06775"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOC50795.1"
                     /translation="MRWSRTLIPTLKETPAEAEIVSHKLLLRAGLVRKLTGGLYTFLP
                     LGLRALRKVEQIVREEMNRAGAIELLMPALQPPEIWQQSGRYETAQEVLYKVRDRAKK
                     EWVLGPTHEEVITTLVAGEINSYRQMPKNFYQIQVKFRDEIRPRFGLMRAKEFIMKDA
                     YSFDATDEAAQASYHKMYDAYARIFQRCGLKAIPVEADTGVMGGKFSHEFMVPAAAGE
                     NEVAFCDGCGYAANVEKATSAVEKCCDQTIEGEVEKFATPGVVTIEALSQPPYEVPAA
                     RQIKTLVYLAASKPVLILLRGDDQLNEAKLTGALGTATFRAAEAEEIFATLGTHPGSL
                     GAVGVTHLPVYADERLRETVEMTTGANADGFHLRHVAIGRDIQVAHWADLRLVRAGEA
                     CPKCGKSLKIQRAIEVGHVFKLGTKYSLAMNALFLDESGKQQPAIMGCYGIGVTRTLQ
                     AVIEQRHDDNGIIWPISVAPYTVCITPLNTAPESAVMQCAERIYAELTECGVDAILDD
                     RNERPGVKFKDSELVGFPIRVGIGEKSLAKGEVEIKPRGGTLVSLKADEAVEKVLSLI
                     ADSAG"
BASE COUNT         5047 a         8908 c         8275 g         5356 t
ORIGIN      
        1 ccaccggcac taaaaaatcc ttctgtgcca ttaaggcctg cttcacttct tccaactcca
       61 atgctactgt ctttacgttc ttttcaacct ttcgggtcat ggttctttgc ctttcgtttc
      121 tgggtcctct attcacccca gtggcttacc atgacccgct gctttttgca gaacttttag
      181 gacactaccc gcgaaaacag ctcctgaaac cgcttgccat ccgcccgcca tctggcaaat
      241 tgtgactgtc cagtgcccgg cataaccggc ggcacccata gctcaattgg atagagcatc
      301 tgactacgga tcagaaggtt tcaggttcaa ctcctgatgg gtgcgccacc tccggccacg
      361 gactccgttt gaccctccgc gccctccgtc cccgcgcccg ccattccgga acctcggccg
      421 acgctgactc ccggccactg catgtttctg ataactattc atacgcccat cactctaccg
      481 cagggggtat gacaattacg ggggtagacc ccgacttttt ccaaaaagtt attcaccgcc
      541 cctgaacctg tcctctgcca aatgcccggt tttgcccgcc cgctcgctct cccgttcccg
      601 aaacgccccc cgcgccccaa cggggcgcca tgcgatagcc cggggcaacg ccttgggaat
      661 agcgccccca aaccaaccca gccctgaaag ggcgaaacac gagccaaccc cacacataac
      721 gccccgttgg ggcggggtgg cggccccgtt tgttggggtg ttggcccggg ctatcaccgg
      781 gcgccccgtt ggggcgcgcg gggccgggcg ggggtggggg gcgtttgcgt gggggtgttg
      841 tgcgggattg gtttgtgttt cgccctttca gggctgggtt ggttgggagg ctttgttccc
      901 agggcgttgc cctgggctat ctcctttggc cgcgttgcgg ccaaaaccga ccccattggc
      961 caccccaccg tcttgccgtg ttgtggccaa cgccgatccc attggcgcac ttcgccgcct
     1021 tgccgcgttg cggccaacgc cgatcccgtt ggcgcatccc ctccatcttg ccgtgttgtg
     1081 gccaacgccg ggcccgttga tgcacctgtt ccgttttgcc gcgttcgaac acgtatcgcc
     1141 gcaccaaagg ccgcataatg attgcccata cccccgcgcc ccaacggggc actctgcgat
     1201 agcccggggc aacgccccgg ggaaaggccc cacaatattt tcaagccctg aaagggcgta
     1261 atttcatatc gcaatccctt gcccaaaccc tcacaccccg ccgccacgaa aaaccgcggg
     1321 cgggtcagga cgatacgagg atggggcccg ggtggctgcc ggtgaggctc cggccgccgg
     1381 cgggcgtgac gaggtaggtg ttctcggtgc ccaccatccc cacgcctggc acgccctttt
     1441 tgggttcgag cgccagcacc atgccctcgg ccagcggctc gtcaaagccc tcggcaatca
     1501 cgggcggctc atcaatttgc aggcccacgc cgtgcccgag aaaggccgac cgccggttcc
     1561 cgcagcccat gaagttcacc tgaaaccgcg ggtcaagctc cgccagggtg cgggcataaa
     1621 tcgcggacgg gatggcgccg ggcttgagga gcgcggccag ccggagctgc agctcgacgc
     1681 attggcggtg ggcctccagc gccgcttccg gcggcggcgc gccgaacata tacgtcatcg
     1741 tcttgtccgt cgcgtagccc tccacgccgc acccgaggtc cacaaacacc caatcgccct
     1801 tttgcaactt gcgctcccgg ctgcccagca ccggcgcggc gctattcagg ccgcggcagc
     1861 cgcccggccc atcgaagctg gtggggtaaa gcgagttctc gccaaaaccc aactgcgcca
     1921 cggcgatgtc cacgttgaac tggctgaagc cgaccgtgcc atcgtgccct tcccgcacca
     1981 tcagcgcata cagctcgcac gccagctccg cctcggtcat cccggcgcgc aacagggccg
     2041 gcgcggcgtc ttcgaggacg cggcggtgca gggcgccggc ccgctccagg agcgccagct
     2101 catacgggcg cttgaccgcc cgcaccccgg cgatgtgcgg gtccaccgcc gccacttccc
     2161 ggcagggaaa gtatttcagg aaccgctgga gcagcgcgaa cggaaccagc ccggtctcga
     2221 ggtggatccg gcgccggggc gcggccgccg cgcccgccgg ggcggcgtcg cggaagcttt
     2281 tcatggggcg gagattggga aagcacgatt ccgcggcggc ccgctcgaag ctgcggcgca
     2341 cccagaacac cgcctcgccg tcgcggggaa ccagcagcac gccatcctgc cgcgtgccgg
     2401 tcaggtagta ttggttgacc ttacccagga acgcggccat ttcccaatcc ggctgcgcgg
     2461 cgtccatccg ggcgcgaaaa gcctgcatcc ggccggtcaa ttccgagagt ggaacttgcg
     2521 ggttcatgcg attgccgccc gcagtatgca aagcaatccc ggcgggaaaa aggaaaattg
     2581 ccgccgcgct ccgcccgggg gaacggcccg cgcgccgctc cgcgcgtccg ccgggcgggc
     2641 ggggaacacg gcggcgtttt ctgaactggc aaattgtggc ggccccggcg gtctgttatc
     2701 ttgccgccat gcaaacatcc tttcgccgct ccgtgagact cgctccgggc cgggcccggg
     2761 cgggaggact gctcgccgcc gccgctctgt tcatcgccgc gcccccggcc ccgcgggcgg
     2821 ccgccccgcc ggagaccgcc gccccggcga acttccgcgc cgggttcgcc gagcgggaca
     2881 tcacgccgga gatcggcatg gaagcgcccg gcaattacgg caaaaactac caccgcagct
     2941 ttcacgaccc ctgcaaagtc cgcgccgcgg tgttcgacga cggccggcag cgcgtggcgc
     3001 tggtgggggt ggacctgctg tttatcaccc gccacctggc gaccggggcg cgcgcggaaa
     3061 tccagcaacg ctgcggcatc cgccccgagg cggtgctcat cggcgcctcc cattcccatt
     3121 cgtccggccc catcgggatg agcgagccgg gcgattacga cgccgccccg ccgctggtca
     3181 agcgactggc caccgagcac agcatcgttt ccaaccccgg ctacacgcaa tggctgcggc
     3241 gccaaatcgt cgaggcggtg gcggacgcgg acgcccgccg cggagaagcc cggctcgccg
     3301 tgggctttgg gcgcgaggag caggtggcgt tcaaccgccg gttccgcatg aagaacggcc
     3361 tcaccttcac acacccgggc gcgggcaacc ccgagatcct cggcccggcc gggccgattg
     3421 acccgcaggt aggcgtggtg ggagtctggg atttgaaagg cggcctgctg ggctgcgtgg
     3481 tgaattatgc ctgccatgcg accaccagcc ccggcggcat ttccgccaac tggatcgggg
     3541 cgatggaaca gacgctgcgc cgggcctccg gccacgccgc gctgccggtc gtcttcctgc
     3601 aaggcgcctc cggcgacgtg acgcaagtgg acaacctgac gaaagtccag aatccccagg
     3661 ccgaggaatg gtgccagttg gtgggcgggc gcatcggcgc ggaggcctac aaaacgctgc
     3721 tgctgatcca gcgcggagcc agctcccaca tccccctcga cgtgcgccaa aagatctggc
     3781 gcatcccgcg ccgcgccccg gcgccggaaa aagtcgcgcg cgcgcgggaa ctggcgcaga
     3841 agcccgcgcg ggaagtcggg gaaccggcct ggaccttcgc caaagaaacc gtcctgctgg
     3901 acgcgctcat cctggcgcgc ccggaagccg aagtcgaggt gcaggccctg caagtgggcc
     3961 cgctggtgct ggtggccaac cccgccgaat acttcgtcca atacggcctc gacctcaagc
     4021 agggaagccg tttccccttc accttccccg tcgaattggc caacggctgc gtcggctacg
     4081 tccccaccga agaggccttc agcgcccagg gcggcggcta cgaaacgcgc ctgacatcct
     4141 acagcaacct gcacgtcagc gcggggcggc aattcctcga aaccggcctc gaactggcaa
     4201 accagatgac gcccggcgcc gtcccgctcc cgccgcccgc gccgccgttc agcgcgccct
     4261 ggtcatacgg caacgtgccc ccggaactgg agtaatcccc ggaacgccgc cccacccggg
     4321 cgcggcccgg cgcaacgcta tcttcgttga ccgccgccac cggcctgata cccttttccc
     4381 ggtggctggg gacaaaccgc tgcccggtta actgacccgc gcgcgcgggc cgccgggccg
     4441 ctctcccggc cttggattac aataaccatg gaagtttctc gcttatgaaa ttactcgatt
     4501 ccccccgctc cggacgcctc ggagcttatg tctattactc gacgcccttt ggccaatgtt
     4561 gccgggcgtt aaccatgccc cgcgacgccc ggtctcccgc ccaggctcgc gcccgcgcca
     4621 gcttcgcggc ctgctcccag gcctggggcc ggagcctgac cgaagcccag cgccagcgct
     4681 gggtggccgc ggcgcaaacc gttcccagcc ggccgtcggt gggccaatac gggcccctca
     4741 gcggccagca attttatgtc cgcatcaaca gcgttctgga ctgcatcggc cagccgcccg
     4801 tcagcgaacc cccggccccg gtggtcttca gccccaatct ggtcaccggg ctggagatcg
     4861 tgcaagaccc ggaagccggg ctgcggctgc ggttgaacgt cggggtggcc acggaagaca
     4921 tcatggtctt tgggcaggct ccatgcagcc cgggccggat gaagcaccgc cgggtctact
     4981 acctgggcct gttgagcccg agccagaacg ggcagagcga catcacggag ctttacaccg
     5041 cccggtttgg gcaaccggca ccggggcaga aggtgtttat tgtcaccagc cagacgcgga
     5101 atggctggaa ggggccgaac tgggtccaca gcgccatcgt cccgctcccg tccacggcca
     5161 aatctcagca agctactgaa aaggcaccag ttaagaaagc gaagcccgcc tccaccttgc
     5221 cctcttccac cgctcgcact accgtttctt cttcgtcatc tcaggctatg tacaagggga
     5281 gtacaccgga ggcgcggcag gagcgcatgg aaaataagca tgaaccccct ttgagcatcc
     5341 ttggtacacc ccttgtacac ggcttattgg gagcgttgaa atggctgggg cgaatgggga
     5401 taagccgggc gacggcgtga tggttatgcc ttgatgattc cctaaatcgg atagcccaag
     5461 cagttcagag ggcgtgcggc tgcctggttc cgcgagcgtg actcagcggc ggaccatgta
     5521 gtacagcacg ggaactgcca tgcggctcat cagcagcgag gcgatttccc cggccatcag
     5581 ggaaatagcc aggccctgga aaatcgggtc ggccaggatc acgctggcgc ccaccacgac
     5641 agccatcgcc gtcaggagca tgggccggaa gcgcacggcg cccgcgtcca cgaccgcctc
     5701 tgccagcggc attccttcgg ccaggcgcaa ttcgatgaag tccaccagga tgatcgagtt
     5761 gcgcacgacg atgcccgccc cggccatgaa gccaatcatc gaggtggcgg tgaagaacgc
     5821 gcccatcagc ccatgcgccg gcagaatgcc caccagcgaa aagggaatgg ccatcatgac
     5881 gaccagcggc gtcacgaagg agcggaacca gccgaccatc aggatgaaga tcagcaccag
     5941 caccgcggcg aaggccgcgc cgaggtcgcg gaaaacctcg atggtgatat gccattcacc
     6001 gtcccatttc agcgccggtt cgctgtccgt gaagggctga tgggcgttgt aaatccgcaa
     6061 gggcgttccc gtgccgccga aaggccgggt atcaatctgg gcgagggcct tattcatctg
     6121 ctggatggcg taaaccgggc tttcgacctc gcccgccaca tcgccaatga cgtaagtgac
     6181 cggcatcaga tttttgtggt agatgctctt gtccgcggcg accgtttcca ccgtgaccag
     6241 ttcccgcagc gggaccaggg cgccatccgg gctggctccg cgaacgcgca gggccagcaa
     6301 gtcctcgggc gaagagcgca acgattgcgg caggcgcgcc tgaatgatca ggtcttcctt
     6361 ctcgcgcggc agatgcagca ggtccacctc cgcgccgccg acggccagtt tcaaagtctg
     6421 cgcgatgctt tcctcgctga tgccgtggag cgcggccttt tccttgtcca ccaggaagcg
     6481 cgccttggcc tggtcggctt cgatatgcca gtcggtgtcc accacgcccc gggtgtggtg
     6541 gaagatttca ataactttgc gcgccagggc cagccggttc tcctcgctgg ggccgtagat
     6601 ttcggccacg agggtttgca ggactggcgg tcccggcggg acttccgcca ccgccacgcg
     6661 cgcgccatat ttgcgggcga tgtcccggac gcggggccgg acccgcttgg cgatctcatg
     6721 gctctgcgcc cggcgctggt ctttggcgac caggttgatt tgcaggtcgg cgatatgcgg
     6781 gccgcggcgc atgaaatagt ggcgcaccag gccgttgaag ttgaagggcg cggcgacgcc
     6841 ggcatacact tgataatcgg tcacctccgg ctcgacgcgc acggcggcgg cgatttcccg
     6901 ggcggcctgg gcggtcagct ccagcgcgct gccctcgggc atgttcagga taatctggaa
     6961 ctcgcttttg ttgtcgaacg gcagcatttt caccttcacc cagcccagcc cgaccagcgc
     7021 catcgccccc agcagcaaca gggtaatgcc ggccaggaac accagccgcc agcgggcccg
     7081 ggcgattaac ggatccatga agcgccggta gaggcgggta aagaaatcct cctcgtgctc
     7141 gaacggcgaa tgcggctggc cggccggggc cgcgcccgcc gccgcctcgg tgacgcgggc
     7201 atacttccga ccccagcgca ggatgcggat ggccgcccag ggcgtgacca tgaacgcaat
     7261 ggccagggac cagaacatgg ctgcgctgga gccgatcggg atggggcgca tgtacggccc
     7321 catcagcccg cccacgaacg ccatcggcag caccgccgcg atgacggcaa acgtggcgag
     7381 gatggtcggg ttgccgactt cgctgaccgc ttcgacggcg atgaccgacc aggggcgccc
     7441 cttgttgtgc ggcagatgga aatggcgcac gatgttttcc accaccacaa tggcgtcgtc
     7501 caccaggatg ccgatggaga agatcaaggc gaagagcgtg atgcggttga gcgtgtagcc
     7561 gtagaggaag aaaatgagca gggtcagcgc cagggtgctg ggaatggcaa tgccgacgat
     7621 gagggactcg cgccagccca gtgtcagcag gatgagcagc gatacgccga ccaccgcgat
     7681 gcccatgtgc agcagcagtt cgtccgactt ttcctccgcc gtctggccgt agtggcgggt
     7741 aatggtcatt tccacgccgt ggggaatcac ccggccctgg agcgccgcca ccttttcgag
     7801 caccgcgcgg gccaccgcga tggcattggc gcccgggcgt ttggcgatgg tcagggtgac
     7861 ggcgggttcc tcgccggccg gcgccgcccg cgccgcgccg gcgccggaaa acacgtattg
     7921 gctcggctcc tccggcccgt ccacgatctc cgccacgtcc cgcaggtaaa ccggctgccc
     7981 gcccggcacg cccaccacca cgccgccggc gtcctgcgcg ctggcgagaa aattgccggt
     8041 ctggagcagg atttcgcggt tttggctaat gagcgagccg gccgcgagct ggcggttggc
     8101 cgcctgcaac cgcggcagaa tctccgccgc gctgaggttg tgcgcggcca gccgggcggg
     8161 gtccagcagg acgcgcagtt gccggcgctc gccgccgatg agcatcgtct cggccaccaa
     8221 cggcacttgc ttcacctggt cgtccacttc ggccaccacg cggcgcagcg tatagtgatc
     8281 gtagccggcg ccgtgaaagg tgagcgccag gatgggcacg tcgtcaatcg agcgcggttt
     8341 gatcagcgga tacgagacgc caaacgggat gcggtcgaag ttggcctgca gtttctggtt
     8401 gagatgcacc aggctgcgcg tgatgtcctc gcccaccttg aagcgcacga tggccaggca
     8461 ctcgccctcc cgcgaggtgg agtagatgta ttcgacgccc gggatttccc acagcagctt
     8521 ttccatgggc cgggtggcgc gctcctcgac ctctttggcg ctgaagcccg gcatggcgac
     8581 cagcacgtca atcatcggca ccttgatttg cggctcctcc tcgcgcggca ggagccacgt
     8641 cgccgccgcg cccagcaaaa tcgaggtgat gaccagcagc ggggtcagct tcgagtcaat
     8701 aaacgcccgg gcgatgcgcc ccgcgaagcc caggtgcggg gcatcagtgg aggaagaagg
     8761 ggtgctcacg ggcgcgcgct atggcgtgga gagtggctgt ccctcgcgca gcgtcgcggc
     8821 gccatccacg acgaccgtct cgccgggcgc gagcccggaa aggatctcga ggccctgggg
     8881 ggtctgcttg ccgctgcgca ccaggcgcat ccgggctttg ccgtcggcgg cgacaaacag
     8941 gatctcaagc tggccccgcc ggacccgcgc ggcgggcggg acgaggagaa cggtggtctc
     9001 gctgaccggc accgccagcc ggccaaattg gcccgcgcgc aggccgggcg ccgccggcaa
     9061 atccactttc acgcgcaccg tgcgggaggc gggatccgcc gccggcgcaa tttcggccac
     9121 ggtgccggtg agcggctcgg ggatggcgtc cacccggacg gccagttcgg cggccggccg
     9181 gatgcggggg gcgagcaggc cggggacatc cgccaccagg cgcagcccgg cgcggccttc
     9241 cagttccagc aacggcttgc ccggcatcgc caggtcgcct tcgtcggcca gtttgcgggc
     9301 caccacgcca tcgaaggggg cggcaatccg gccgtagccg gccagcgctt cggcctcggc
     9361 caccgccgcc tgggcgacct ggtggcgggt ctgcgcggcg tccaattcgg cctgggtggc
     9421 cgcgctttgc ttgtggagcc gggttgtgcg gttcaagtcc agctccgcct gccggagcgc
     9481 ggcgcgggcc tggtcgagcc gggcctgaat ctccagcgtc gccagttcca ccagcacgtc
     9541 gccctgtttg acggattgtc cgagcgcggc ggacaagcgg gtgatgcggc cgctgacctt
     9601 ggcctcgagc acggcctgga gcctgggaag cacggttccg acgacctcct ccagggcggg
     9661 ctggctcacc gcttcgattt tctgcaggcg cacggcggcc ggcggcaagg acggcccggc
     9721 gcccgcgggc ggcgccggct tttcgccgca gccgttgagc agggcggcca ggcccgcgag
     9781 cagcaggccg gagcggaagg ataatgcgta cgtggggatg gctttcattt tgcgggggtc
     9841 tcgaaattca gttgcggcag ccccagcgcc ttgcgcaagg cgccaatggc tatttgccag
     9901 tcggcctcgg cctcggcgcg gcgaacgcgc gcggtgatca gcgcggtttc cgcgtccatc
     9961 aagtcccggg gcagcgccag ttcctgctcg aagcgggcgc gggtcagggt ggcgctttcc
    10021 tgggcctggg cgacggcgtg ttcggtgacg gcccggcgct ccgcggcggc ctggaggtcg
    10081 agccgggctt gcgcggtttc gagatcgagc gcgaggcgca gtttgcgctg ttcggcacgg
    10141 gcggcttcga gttgggcgct ggcctcgcgg actttggcgc gcgtgagttg gccgtcccac
    10201 aggtcccatt ggagcaacgc cccggcggtc caactgccgc cgccgtggtt gaatttccag
    10261 ccgtggtcgt aatccaggct gccaaaggcg ctgatgcgcg gcagatagcc gcttcgggcc
    10321 tcgcgcacct gttccgcggc cgcgcgttcg cggtggcggg aagccgccag ctcggggcgt
    10381 ccggagaaat ccccggcgtc gggggcctcg agcgccgggg cggtgtcggc gacctcgaat
    10441 tcatccggat cctccggctc cacgccgagc aaagcccgca gcgcgcgcgt cgccagcgcc
    10501 tcggcgttgc gggcgcggac caggtcctca cgcgcctggg ccagccgcac ttccaggtcc
    10561 agcacgccgc ttttgaggag cgtgccgctg tcatggcgtc ggcgggcgac ggccaggctg
    10621 ttttcgagcg cctcccgggc ggcgcgggcg gcgcggacaa actgccgggt tttaagcacg
    10681 gtatggaagg cgcgggtcac ctcgaagccg agggcgttgc gaagggcggc ctcgtcctgc
    10741 cgggcggcgt cggtgtgcgc gcgggccgca tggcgcgcgg cggtggtgcg cccgccggcg
    10801 tacagcggca ccgtggccag gccgcggacg ttcaggttgt caaccgcggg caggtcgttg
    10861 aaatcggggg gcgaaccgta gttgtaggcg cgctggttga ggatgctgcc gaacgcctgc
    10921 atcgggttgt cgctgccggc ataactggac tggaattgca ggcgcggcca aaaggcggcg
    10981 ttggcctggt cgagcccggc ttgcgccgcc gccaaacgat gctgggccag acgcgcgtcc
    11041 gggttgtccg ccagggcctg gcgcagggcg cgctccaggg tccaaggctc agcggccagg
    11101 gcggcccagg cccccagcag cgccagcccg cagatcgcca cggcctttgt catttgctgc
    11161 agcaagcacg gggggcgggt gcggatgaat cgtcgcggcc aacgttggcc ttgcgcaaaa
    11221 tcacctccaa cgggcaaaag ccggtgaagg aggattgcag caggttgaac cccacaaacg
    11281 cggtgaacca aagccagtag gggctgtggt aatgcgccaa caggaggctc aacaggataa
    11341 agctgccggc gaagcggcga atgatcatgt gcatcttcat aacaattcag gcttgggttg
    11401 ttctggctta ccgaatactg ctggcaatca gggccccaac caggccgcca tagagcgtac
    11461 tgatcagggg attgctcgtc aacgggcaag cgccggttga gcatccgaca aacttgtaga
    11521 gggcaaaccc cagcccgccc ccggcgatcg cgccaatgat aatatgcaga atcatacgat
    11581 gtttagtctt caatcactac gcctattgag ccgcgagccg gcggaaagtt acaggtgttg
    11641 acccaattgc ggccgccgcg gggaggcgga aatgcggcgt tcccgccttg accgcgcgcc
    11701 cgcgccgcgc caggattccc ttgctatgga aatgaaactc accgcggtcc ggattgagaa
    11761 acccgagccg atcaacttca tcctgggcca gacccatttc atcaaatccg tcgaggacat
    11821 tcatgaggcc ctggtggggg cggtgccggg catcaagttt ggcctggctt tttgcgaggc
    11881 gtccgggaaa tgcctggttc gcgagtcggg caccgacccg gccctggttg aactggcccg
    11941 ccggaatgcc ctggccatcg ccgccgggca tagtttcatt gttttcctgg gggaaggttt
    12001 ttaccccatc aacgtgctca ataccctcaa ggccgtgccg gaggtttgcc gcattttctg
    12061 cgcgacggcc aatcccacgc aagtgctcat cgcggccacc gagcaagggc gcggcattat
    12121 cggggtggtg gacggctcgc cgccccgggg cgttgaggcg gagccggaca ttgcgtggcg
    12181 caagaatttc ctgcgccaaa tcgggtacaa gctttgagcc gagcggcgcg cgcccggccg
    12241 gggccgggct gtgttacccc tcccgcgccg ggttgtccag gcactcccgc accacgttgg
    12301 ccaggtcggg gcgcgtatag ggtttctgca ggaacagcgc gccatgccgg cggaagaagt
    12361 ccgtgtctga cttctccatg ttatagccac tggtgaagat cagtttaagc tggggctgga
    12421 tggctttcag ctttgccgcc agttccaggc cggacatgcc gcccggcatg accatgtcgg
    12481 tgaccaccag gtctatgccg cccgcctgcc gttcccaggc ttgcagggct tcggcgcccg
    12541 agctggccac cagcacccgg tagccgcaat cgtgcagaat gagttgcgcc aggtcgcgca
    12601 gcacgatctc gtcttccaca acaaggatgg tttcgtggcc gccccgcacg gcggccgctt
    12661 cggtcgtcct ggccgcccgc gccggggccg gctcgtcgct ggcgggcaga tagacgatga
    12721 aggtcgtgcc ttttccgact tcgctgctga cctcgaccca gccttcgtgc tgcttgacga
    12781 tgccatagac ggtggccaat cccaggcccg tccctttgcc gacttccttg gtggtaaaga
    12841 agggctcgaa gatgcgggcc attgtcgcca tgtccattcc gcatcccgtg tcgttgacct
    12901 ccaggcagac gaacgcgccg ggccgggcct gggggtgcat ttgcacgaag gcttcgttca
    12961 gcatgacggg ggtggtgcgg attgtcaggg tgccgccttt cggcatggca tcgcgcgcgt
    13021 tcacggccag gttcacgatg acctgttcga gcatgcccgc atccgccgcc accaggggca
    13081 gctccgccgg cggattgaat tgcagcgcga cggtttcccc cagcaggcgc ttcagcatct
    13141 tgctcaaatc gccgacgaca ttccgcaggt cgagcgcctt gggctgcatc acgttcttgc
    13201 ggctgaacag gagcagctgc cgggtcaatg tggcggcgcg ttccgcggca aaatagatgg
    13261 cttgcgcgga atcgagcagc gcgggcgcca ggtcggtgtt ggccatcacc atccccgaat
    13321 gtccctggat gaccgttagc atgttgttga aatcatgcgc caccccggcg gcaagctggc
    13381 caatggattc catcttctgc gactgccgga gctgcgcctc caggttcagc cggtcggtga
    13441 tgtcttcgac gtagcaatgc acgatgtggc tggccgccac cggatgaaac gaccagctca
    13501 gggagcgctt ttcgagcacg gtggagagat cgagcacgct gcgccccgct ttcaggcagt
    13561 tctggacgat ttcctgcacg ttgtccggca gcaactcccg cggatggtcc cggcccacgg
    13621 aacggctcag cttcagtgcg gcgttgttga aataggtgat ggcgccgtcg ctggtcagct
    13681 ccaaagcggg attgggattc aactgggcga aggcggccag cttctgcaat tccgtttcaa
    13741 cccggatccg ctcgaccgcc atggccagca ccgtggcgat ggaaaacagg aaatgcacct
    13801 catcctcggt aaacgtgcgc gggcgtttgg tgtacgcccc gagcacgccg aacgcctgct
    13861 cctggccggc tatggccacg gtaacgccgc tcaccacttt gtggttggtc agcaacgatg
    13921 acccgcggaa ccggccctca ttgccaagcg aggcaaacgc caccgcctcc ttcgccgtga
    13981 gggcaaatcc cgtctcggtc tgcggatccg ccgggatgac taccttcccg acctcgccgt
    14041 ccttccagcc cacccccgcg cgcatcagga gaaacttgcc tccgggctga acctccagaa
    14101 catggcagaa ctccacgtcc agcgtttggg ccgccagcgt ggcggcatgg ttcaggagga
    14161 tggagggatc gctggtttcc agcgcaaacc gccccagtgc cgccacggct gtttgctgca
    14221 tggtccggtg ggtcagcgtc cgtttcaccc gctcgctttc tttctcccgc tcctccagtt
    14281 gggccgccat cgaatcaaac gtgcgcgata attcgcccaa ctcgcctcgt tcccgggaca
    14341 aaccggtccg gctggtcagg tcgccggcgc ccagtttccg cgccgccaaa gaaagcaccc
    14401 gcacctgccg gataataaag cgttccccgc caaaccacgc ggctcccagg gccagcagac
    14461 cgaccaggaa ctcggtccag ggcagttgcg cgccatagtg ggacgcatag tatttgttcc
    14521 cgaagtagag cagcacagaa gccggggcga cggccaggaa cactgttccc accaaacgaa
    14581 atcgcaagct ggacaagctg gtcataagct ctggacgttc atttgctact ggccgagcat
    14641 acccggttaa ccacctttaa cgcaaaaaga tttctaacgg gtggcgtccg gcgtttaaca
    14701 acgcccaccg gggtgaaagt aagccaataa cggtttcggc cgcttccggt gtgaattgcg
    14761 acacggcctt tcgcccgctc ccacccctgg cccgctcttg gcaacccttt tgattccaag
    14821 cccatcggac gagggcggtg aaccgggaag gcgtccggct gggaggtttg cgcgggatat
    14881 tccctgtcct ccttgccctg ccagcctccg tatgcgcgga cactccgctc gctttggcgg
    14941 ttttgtagcg gcaagcaagc aggtggcccg cgcgccctcc gcgcagggat gctttctaaa
    15001 acttgccgat tctttggctt gccaaacgcg ccgccatctc tagattaagc cacttgttta
    15061 acaatatgag tctatttagt cctaacgctc tgctggcgtt ggcgcccccg gcgcagcaac
    15121 cgggtcagtc tgcgccgccg gcgtgggtca atatggtgcc gttggtcctg ctggtcgtcg
    15181 ttttctattt cgtgcttatc cggccgcagc agaagaaggc caaggatcat gcgaccctcc
    15241 ttaaaaccat ccgccccggc gacaagattg ttaccagcag cggcatcgtt gccgtcgtcg
    15301 tcaccgtcaa ggaaaagacc ctgtccatcc gctcggcgga tgccaagttt gaaattacca
    15361 aatctgccgt tgctgaaatc actgaacgcg gcaacgattc ccgcgaatcc tgatctgttt
    15421 ccatgaaccg aaattacttc tggcgaactg tcctggttgt cctcattgtc ttgtggtcgc
    15481 tctatgagct ttacccgccc aaaggacgcg acctggtgca gtatttccgc gatcaggcag
    15541 tcaatcgcga tcccgacttc accaccatct tcaccaatgc gttggccctg caaaaggcca
    15601 tgccggaaaa gccttacgaa aacctgctta aggccatcga caccaatgac atcacccgct
    15661 atttcccgtc attcggggcc aaagagcaac tgcacccgac cccttacatt ctcaaccgct
    15721 tgcagcgcca ggccgccggt cgcattcgcc tgggtctcga cctgcagggc ggcacttcct
    15781 tcctcgtcag catggatacc aaccaactgg cccaggctgc ggacgccgaa aacgcgctct
    15841 cgcacgccgt cgaagttctg cgcaagcggg tggataaatt tggcgttgcc gaacccctga
    15901 tccagccgca aggcaataac cgcattctgg tccaattgcc cggcttatcc gccgccgacc
    15961 aggaaagcgc caaagcggcc attcaaagag cggcttttct ggagtttcgc ctggtctgcc
    16021 ccaccagcga acaggatatc aaggaaggga ttgtgcagcc gggctacgaa atcctcaaac
    16081 gcaagcagcg cttgcgcgac gggcgcgaac atatcgaaga ggtggaagtc aggaaacgag
    16141 cggaaatgga tggcagcggg ctcaagagcg ccatggtcgt gcgcggcaac ctgggggagc
    16201 cggaaatcca tttcacgctg gattccaagg gcgccgagcg ctttgccgaa attacccggg
    16261 agaatgtcgg ccagcgcctc gccatcatac tggataagga gttgtattcg gcgccggtca
    16321 ttcaaagccc catcgagacg gggagcggcc agatcaccgg ccaattcgat aatcgcgaag
    16381 cctttgaact ggcgaacgtg ctggaaaacc cgctccgggc gccgctgcat attgatgagt
    16441 cgcgcgaagt ggaccccacg ctgggcaaag actcgattcg cagcggcatc aacgcggcca
    16501 tttacggcac gctggcagtg gcggtgttca tgatctgcta ttacctcgcg gcgggcgtgg
    16561 tggccaacct tgcccttatc gccaacatta tcattctgct gggggtcatg tgctccattg
    16621 gcacgacgct tacgctgccg ggcatcgcgg gcatcgtgct caccattggc atggccgtgg
    16681 acgccaacgt gcttatttac gagcgcatcc gcgaggagag cgccaaaggc aagtccctgc
    16741 gcggggccat tgccgccggt tattcccgcg ctttcaccac catcttcgac tcgaacctga
    16801 cgaccctcat ctcttcggtg atcctgatct acatgggcac cggtcccatc aagggctttg
    16861 gcgttactct gactattggt attatggcca gtttgttcac ggcgctggtt gtcacccgcc
    16921 taatcttcga cttcctcgcg gaccgtggtt ggctcaaatc cttgcccatg ctgcatctta
    16981 tccgggccga gaagatagat ttcctgaaat tggcacccta cacctttgcc atcacctgga
    17041 tcattattgt ggtgggggta atcttcacca ttcggcgggg agaagcagcc ttcggtgccg
    17101 aattccgcgg cggggatacg gttctgatgg ctttcgagca aaaaccggga gaagccgccg
    17161 tgcgaaccgc cctgaccaaa gccggcatca aagacccgcg cattcagttt cagaaggatg
    17221 tcaccacgcg gcgcgaaacc ctgcgtatag attcttctcc cggaacggcg gaactggtca
    17281 aatccactct cagccagatg ccggcggcca aattccatct gctgggccag gatcgcatcg
    17341 gcgcgacaat agggaaggaa attcaaaaat ccgctgcgat cgccctgttg ctctccttgt
    17401 ttggcatcct gatttacgtc gcaatacgct atgagttttc gttcgccgtt gcggcggtca
    17461 tctcgttgat ccatgacgtg ctgatcaccc tgggcctcta ttcgctctcc ggccgcgagt
    17521 ttaactccgc cacgctcgcg gccatcctga ctatcatcgg cttctccatc aatgatacga
    17581 tcgtgatctt tgaccgcatc cgcgaagatc tgaaactggg cattcggggc tcgttccgcg
    17641 aggtgatgaa caaggcgctt aaccaaaccc tcagccgtac ggtgatcacc tccggcaccg
    17701 ttttcctgtc aacctcttcg ctgtatgtgt tcggcggcgg cgccatcaac gactttgcct
    17761 ttacgttcct gattggcatt gtggtcggca cctactcgag tatctacatc gccagcagtc
    17821 tcgttctctg gtggcaccgg gggcagcggc cgagcatcgg cgctggcgcg caggccgtca
    17881 tgcagaacgc aacggcagcc gctttgaagt aatccttgcc tttataaccg gtttccgagg
    17941 actgggggcc gggatgagct tgtgatcggc ctgataaaca ttactgcttg gcactgggcg
    18001 ggttttatcg gctgcgtatt ggtttttctg gccctcgacc tggggctgtt tcatcgccgg
    18061 gcgcatgtgg tcaaattccg ggaggcgctg ctttggacct ccacttggtt ttgcctggcc
    18121 atgctcttcg ccctggcgct gaaacccctc cgcggcaaag aggaagccct ggaatttctg
    18181 accggttatc tcatcgagtt gtctctctcg atggataatg tctttgtgat tgccatgctc
    18241 ttcgcctatt ttcaaatccc tcgccaatat cagcatcgcg tgctgttttg gggcattgtt
    18301 ggggctttgg tcatgcgtgg gcttatgatc ggagccggcg tggcgctggt tgcctggctg
    18361 cattgggtgc tctacgtgtt gggagccttc gtcctgttta ccggcctcaa aatgcttttt
    18421 gtcgagacca gggttcaccc ggaaaagaac cgcgtcgtca aatgggtgcg caagctttac
    18481 ccggttacgc cacacctgga tgggcaaaag tttttcagca cctggcaggg gcgcctggcc
    18541 ctcacgccgc tggccctggt gctggtgatg gtggaaacca ccgatctcat cttcgcgctg
    18601 gactccattc ccgccatttt cgccgtaacc acccggccgt ttattatctt cacctccaac
    18661 gtgtttgcgg ttctggggtt gcgctcgctt tattttgtgc tggcgggtgc tcttgagtat
    18721 ttccgttatt tgaaagtcgg cctggcgttg gtgctggcct ttatcggttg caaaatgctc
    18781 cttgatcccc acggcggccc cccgctctgg ttccaactga agattcccat ttctgtttct
    18841 ttgttcgtgg tgggggggat cattgttgct tccatcgctc tctcgctcac cctgggccgc
    18901 cagcaaccac ctcgctccgc ctcatgaagt tccggtggtc tctcgcgccg ccgcaacgcg
    18961 cccacgccga acgcttggcc gaggcgctga agctcccacc gctgctcgcc caatgcctgc
    19021 ttaatcgaaa cttgacggaa ccgtcgtccg ccgccgcttt tctgcgcccc cggctccggc
    19081 aactggccga tccgttcctg cttccccaca tgcctcttgc cgtcgaacgg ctcctgcagg
    19141 ctcgcgagcg caaggagccc gtcgtcctct ttggggacta tgatgtggac ggcgttaccg
    19201 ccagcgcatt gttgctcgaa gtgcttcgtc cccttggctg gcaagtccaa tattacctgc
    19261 ctcgccgctt ggaggaaggg tatggcctta gccaggaagc cgtcgaaaat tgtctggccc
    19321 aatctggcgc caccctgctg ttagccgtgg attgcggttc gacagcggtc gctccgattg
    19381 cctggttgcg ccagcgcggc gtggaggtca tcgtgctgga ccatcaccaa atctcggtgc
    19441 ccgcgcctgc ggccattgct ctggtcaatc cacggctttc cgcgtcctgc aataccagcg
    19501 aatccgagga ttccggcgcg ccctttacag agctctgctc cgttgggctc gccttcaaac
    19561 tcgcccacgc tctgcttaag cgcgcccgcg aaaccagtat cctcggcgcg gctgacttcg
    19621 acttgcgttc cttgctggat ctggtcgcct tgggaaccat tgcggatgtg gtccccttga
    19681 ctggcgaaaa tcgcattctg gcatccgttg gtttggaacg cctgagcacc actcagcgcc
    19741 ccggcctggt ggctctgaag caagttgcgc agtcccctgc cctgcttggc tcccatgagg
    19801 ttgccttcca actcgccccg cgactcaatg ccgccggtcg tctggaaacg gcggaggaag
    19861 ccctgcgcct gttactgacg cgcgaccatg ccgaagcgag gactcttgcc caaacgctcg
    19921 acgtccgcaa ccggacacgc cagaaaatcg agcgcggcat tgtggaagat gccatcgcca
    19981 ccgtacagaa cagattcaat cccagcacgg attatgttat cgtcgagggc aacccgacct
    20041 ggcacatcgg cgtggtgggc attgttgccg cccgcgtgct tcaacaattc tatcgcccaa
    20101 cggtcatact cggcagcgat ggcgcgacct accggggctc cggccgcagc attccaggct
    20161 tcaacctggc cggtgctctg cgcgagtgtg ccgacctgct tcttcgccag ggagggcatg
    20221 ccatggcagc cggggttagt cttttacctg ccaacctcga ggcttttcgc gcccgcctca
    20281 accaacttgc cttgaatact ctccaacccg aggaactcca gccttcgctg cgcctcgacg
    20341 ccgaagtgcg cctggtagat ctgcattggg aatgccttgc ggcgctcgcc cagttgcgcc
    20401 ccacaggcca gaataatcct cccgttcaat tgttcgcccg caaactcaac ctgcatcgcc
    20461 ccccgctcca catcggtgct caaaaacagc acctcaaatt gtggattaac gacggaacta
    20521 ctgcgctcga agctgtttgg tggaacgccg ccgatcaaac tatacccccc gcccctttcg
    20581 acttggcctt tgcaccccaa attaacgaat acaacggcag ccgcagtgtt caactgaaga
    20641 tcttggattg gcgtcctgcg caataaaaca atccccattg gaccaacctc gggaacactt
    20701 gagtgggaat gcaggaagtt gcatcatcat gtgaaatggg gcgtgtgttt ctaaatctgt
    20761 ttctctttca acgcaccgcg acgataagcg cagaatccaa atgccgctaa ctgcttcttc
    20821 tatttcgggc aatacacccg gagcttgaat ccggcaactg aaccacgcac tcttccgtct
    20881 atctatggtc tccgccatca tcgttgccgc cggccaaagc tcccgcatgg gtgccggagt
    20941 ggacaaactc ttcctcaaat taaatggctg ccccatcgta gcgcacacct ggcgtcagtt
    21001 tgagcttgcc gaatgtattg acgaaatcgt tttggtcgtg cgcgagacca tgagacccgc
    21061 atttgcggcg ctaggcgaag aataccggtt tcacaagccc ttccgcctcg tctccggcgg
    21121 caaggagcgg caggactcag tctggaatgg gctgcaggct ttatcgcccc atgttgagat
    21181 tgctgccatt caagacgcgg ctcgaccttg cacctccagt gcgctcattg cggcgaccat
    21241 cgccgctgcc cgtcaaaccg gcgctgccgt ggccgcccaa gccatcacgg ataccatcaa
    21301 agagtcgagc gacggcaagc tcatcgaacg cacgctagat cgctcacggt tgtggtccgt
    21361 gcaaacgccc cagacctttc gggttgagat tattcggcag gcgctggcgg cggtgcgccg
    21421 gcaaggcctg ttggttaccg acgatgccgc cgcctgcgag ttgatcggtc aaccggtcca
    21481 actggtcgcc tgcctccagc ctaatcccaa agtcactcgc ccggaagatc tcccctgcct
    21541 cgaggcactg ctgcgcgccc cagcccttcc gtagcggggc tcacgcaaag caggctgggc
    21601 tacttggcgg cctgctaccg gagtcaactc cccagaatgc tcccggttgt gagttaatca
    21661 ccggaagacg ccttggccca agctgggtat cgaggctcta ctgccattag gaaggaatgc
    21721 cctcgcctgt tacaaaagtt tggtctgacc cgggatggca acaggataga gcccgtcggg
    21781 gccgggtttt accggcggtt cggtgttcat tgtaatttcg cctattggtg ggaaggcgaa
    21841 tttggaatca agaacttcct gcaaactgat cttcttaccc gtatacaccg ctagttgtcc
    21901 caatacggag accagcgtcg agtcggccaa aattttggcg cgctgaaacg gcttgccagc
    21961 acggatggat tgcaggaatt cgctgtgttc caattcgtaa ggcgacttcg cagactgttc
    22021 aacattggca cgccaagtgc gattgccgtt gaggtcggta aaatacggcg gttgaaatgc
    22081 cgtaaagttg cagcggccct tggtgccgtg aaaaatatcc tggtttgagc cataacaacc
    22141 gactgccgtg cggcaggcac cgtagatgcg gcggccgttt ggatactcgt aaataacctg
    22201 gttatgatcg aagcaggtgc ccatcgtggc ttcgaaatgt gtcgagcggc cccccatgcc
    22261 gtaagcttgg cttggcagca cgtcgcccag cacccacagg gcagaatcga gatcatgaat
    22321 gagcgactgc agaatgtcat cgccggacaa ccacgaaaag tggtaccagt tgcggagctg
    22381 atactccatc tcgctccatc ccggctcgcg ctcaatcaac cgataaggcg agcgcaggta
    22441 ttcacattga acggctacga tttctcccag ttcgcccgct tggatgcgtt ggacagcctc
    22501 acgccggccg agatgataac gataagtcac gcccgccagc acccccgtgc cattttgctt
    22561 ggacaatacg tcagcctgca tgatcttgtt gacgccagca atatctacgg cggcgggttt
    22621 ttccacgaat acgtgtttct tgttctgcac tgctgccagg gtgtagtccg gatgaaaccg
    22681 ggaagcgcaa accaccaaca cgacgtcaca gttttcaatg acgccgcgat agccctgcaa
    22741 cccggagaac tgccgcccac gcggaacctc tatccggtcg gggaacttcg gactcaaggt
    22801 gttgatcgtg gtgtctattt tatccgggaa gatgtcagcc agcgcccaga gtttggtccc
    22861 tgcgtcaacg gtcaacgcat tgcccgccgc gccggtgcca cgcccgccgc aaccgatgag
    22921 tccaagtttg agcgtgtcgc tgcctgccgc gtgagcaaag cggcccacat caatggcgcc
    22981 gatgagcgcg gttcccgcca ccgtggctga ggtcttaagg aagcctcgcc ggctggtaaa
    23041 tctggtttgg ttttgctgtg cagtggtctt cattgttaat ccgttcgttg tctaaatgtg
    23101 cgcctatact aatctactgc cggaacggca ccagcaaaaa aacatatttc ctctcgcgtg
    23161 cgcacttcaa tttcagcaac ttcctcaggg ggaaattaga taaaaatcat gggggttttc
    23221 atacttcgcc tcacgctccc acgatttgcc cccacccttg ccaactcccg cctccactat
    23281 taccctgagc tgaacgattt tcgtgattat gaaacaacac ctgcttttcg gagtgttggt
    23341 gacggcgctc agcatcaact tggtgattgg cgcccgggtc tacttgaatt ccgcgcaagc
    23401 ggcggaaaag gattcggcct atcccagcct tgaactcttt tcctacgcct tggaaaaggt
    23461 gcgcaaggat tacgtggacg ggcaaaacct gacctatcgg caattggttt atgcatccct
    23521 caaaggcatg ctcagcaccc tcgatccaca cagcgaattc atggaaccgg ccaagtacaa
    23581 ggaactccaa agtgatacgc agggcgagtt cggtgggctg ggcatcgtga tcgccatgcg
    23641 tgacggtttc atcactgtga tcgctcccat ggaagacagc cccggttttc gcgccggcat
    23701 tctctcgggc gatcgcatca ttaagattgg agagcgaagc actgaaaaga tgagtttgca
    23761 ggaagcggtg acaaacttgc gcggcgaggc gggcaccgag gtaaagattt ccgtcctgcg
    23821 cccttcttct ggcaagatca aggactacac gctcactcgc gccgttatca aggttgacat
    23881 ggtcaaggac atcaacggca aaaaggagtt tctgttggat gacaataaaa tcggctatat
    23941 ccgactggtg cagttcggag aaaagaccgg tgccgaatta gagggcgcaa tcaaaaaact
    24001 caaggctcaa gggttgcaag ggttggtgct ggatctgcgc tggaatccag gcggtttgct
    24061 ggagcaagcg gtagatgtct gcgagcgatt cctcccgcgt ggcaacctcg ttgtcaccac
    24121 cgaggggcga aatcccaccc aaaactcggt tcgcaaagcc cggggtcgcg gcgacgaatt
    24181 aagcggtttg ccaatggtag tgctggttaa cctcggcagt gccagcgcct ccgaaatcgt
    24241 cgccggctgt ctgcaggata gcaaacgtgc catcatcctt ggcgaaaaga ctttcggcaa
    24301 aggttccgtc caaagtatca tcccgctggc tgcgatgtcg gacggcgttc ccgccttgcg
    24361 cttgactaca gccaaatatt atacccccag ccataaggtc attcacgagg aaggaattac
    24421 tcctgacatc atcgtgcccc tgtctgaaga acaggagcgc tttattctaa tgaaacatgc
    24481 cccaggcggg attcagggga tggaagagag tgagcgggaa caagtcttga gatcgggaga
    24541 cccgcaactc gaacgcgcca tggatctgct caagggcatt actcttttta cccagcggtc
    24601 ccccgctttt aatcagagac ttgccaaaag caaaacacgg gatgccaagt aattccaccc
    24661 ccgcaaaagc ggcgcgcaac cggtccgtga accatgcccc ttctggcgct ggaaacatct
    24721 tgtgacgaaa ccagtgcggc cgttgttgac caaggtcgcg tcctggcgca tctcgtttcg
    24781 tcccaggttc aactccacgc cgaatatggt ggcgtggtgc cggaattggc ggcgcgagag
    24841 catctccgcc atttgttgcc tgtcactcga ctggccttac aaaaggctgg cgtggacccc
    24901 gaacaactcg acgccgtcgc agcaacccgt gggccgggct tgccaagcgc actgatgact
    24961 ggcttcaagg cggcgcaagc ggctgcgttt gcactgcgtc ggccttttct cggcatccac
    25021 caccatgagg ctcatctcta ttcgccttgg atcaacggcc cgccgccgaa agccgatttc
    25081 tcccgttttc agcccaatat ttcccttatt gttagcggcg gacatactct cttggttcat
    25141 gtccgctcag agttaaacca ccacctgatc ggctcgactg tagatgatgc tgcgggcgaa
    25201 tgtttcgaca agactgggaa actgatggga ctgccttatc cggcgggacc agagatagac
    25261 cggctcgccc aggacggaaa tcccgaggcg ttcgttttcc cgcgtccgat gattgatgag
    25321 ccgaatgacg attttagctt tagcggtctg aaaacctccg tgcgatactt tctgcgcgac
    25381 caccccaaac tgctccagga tacacaatgt ctgcgcgacc tctgcgccag cattcaggcg
    25441 gccattgtgg acgtgttggt gactaagacc ctccgtgctg ccagacgact ttgtgtggga
    25501 tgcattacgg tctcgggcgg cgtcgccagc aatcgcgcgc tgcgccagcg cctcggtgcc
    25561 gcctgcgaac ggcagcggtt cgccctgatg ctggccgata aagccttttg cacggataac
    25621 gccgctatga tcggcattct ggccgagcgc aagctccgtc tgcattcgga accaactccc
    25681 ctcgatgcgg atattctgcc aagctgggcg ctggaataac atagcccggt gcccaccgca
    25741 ctcccatcaa tcggcctatc cggctgaatc cgcaatcaat gaaagaacct tttccaccgc
    25801 ctcatcagct ttcagtgaca cgagggtgcc tccgcgcggt ttgatctcca cttcaccttt
    25861 ggccagcgac ttttcgccaa tgcccacacg gatagggaag cctaccaact cggaatcttt
    25921 gaacttcact ccgggtcgct cgttgcggtc gtcgaggatc gcatccacgc cgcattcagt
    25981 cagttcagcg tagatccttt ctgcgcattg catgactgcg ctttcagggg ctgtattgag
    26041 tggtgtaatg cataccgtgt aaggcgcaac gctaatgggc cagataatgc cattgtcatc
    26101 atggcgctgc tcaatcaccg cctgcaaggt gcgcgtgacc ccgatgccat agcaccccat
    26161 gatcgccggt tgttgtttgc ctgactcatc gaggaacaga gcattcatag ccagactgta
    26221 cttggtgcca agtttgaaaa cgtgcccaac ctcgattgca cgctgaatct tcaacgactt
    26281 cccacacttc ggacacgcct ctccagcccg taccaaccgc aggtccgccc aatgcgccac
    26341 ctgaatgtcg cgtccgatgg cgacgtggcg caagtgaaac ccatccgcat tggcgccggt
    26401 ggtcatctcc accgtctccc gcagccgctc atcagcataa accggcagat gagtcacacc
    26461 aaccgccccc agactacccg ggtgagttcc cagtgtcgcg aaaatctctt cggcctccgc
    26521 cgcacggaaa gtcgcggtgc ccaacgcgcc ggtcagcttg gcctcgttca gttgatcgtc
    26581 ccctcgcagg agaatcaata ctggcttact ggcggctaga tacaccagcg tctttatctg
    26641 gcgggcggcg ggcacttcgt atggcggttg gcttaatgcc tcaattgtca ccacgcccgg
    26701 agtggcaaac ttttcaacct cgccctcaat agtctgatca cagcactttt cgacggcact
    26761 ggtcgccttt tccacatttg ctgcataccc gcatccatca cagaacgcga cctcgttctc
    26821 tccggcagcc gccggcacca taaactcatg tgaaaacttc ccgcccatca cgcccgtatc
    26881 cgcttcgacc ggtatggcct tgagtccgca acgctgaaag atgcgcgcat aagcgtcata
    26941 catcttgtga taactcgcct gcgcggcctc gtccgtagca tcaaaactgt aggcgtcctt
    27001 cataatgaac tccttcgcac gcataaggcc gaaacgcggg cgaatctcat cccgaaactt
    27061 gacctgaatc tggtagaagt tcttgggcat ctgccggtag gagttgattt ccccagcgac
    27121 gagtgtggtg atgacctcct cgtgcgtcgg cccgagcacc cactcctttt tcgctcggtc
    27181 acgaaccttg tacagcacct cttgggcagt ttcgtaacgc ccgctctgct gccagatttc
    27241 cgggggttga agcgctggca tgagcagttc gatcgcaccc gcccggttca tttcttcccg
    27301 tacgatttgc tcgaccttgc gcagcgcacg cagccccaat ggcaaaaagg tgtagagccc
    27361 tccggtgagc ttgcgaacaa gcccggcgcg caataacagt ttgtgagaga ctatttcggc
    27421 ttcagccggt gtctctttca gcgtgggaat taatgttcgt gaccaacgca tacgagcagc
    27481 ggaatgtgga ttgcccagat cgttttggcg agcaagaaat accgcccgca gctggcaagc
    27541 ccccatttat tgatccagtt gctatgagag ttttggttca tggttt
//
LOCUS       DAOPQG010000044        27021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2281_length_27021_cov_15.215234, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000044 DAOPQG010000000
VERSION     DAOPQG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 27021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27021
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2281_length_27021_cov_15.215234"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(655..2052)
                     /locus_tag="PKI07_06780"
     CDS             complement(655..2052)
                     /locus_tag="PKI07_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOC50796.1"
                     /translation="MVLPFVVLLGTIALAPLLCPDWWSRHYPKVALSLGATTLAYYLF
                     VLRAYERVLLGGGEYVSFIVLIGSLYVVSGGIHLHVRGEATPRANTLFLLLGALLANV
                     FGTTGASMLLIRPWIRMNKYRITAHHIVFFIFIVSNVGGCLTPIGDPPLFLGYLKGIP
                     FWWVAEHCWPIWAVGVGFLLALFYVLDARNYRRAPRAVRERLAEPPDQWRCEGLGNLA
                     YLLVILVAVFISRPLFLREALMLGAAAGSWLTTRPRIHAANHFDFRPIQEVAILFIGI
                     FATMMPALDWLQHNASRIGAPAPAVFYWSSGILSSLLDNAPTYLSFLSAAFGLFVDPE
                     IIDKVQQLVQQSGAGLAALTGPQAEAIGNTYAALQKYHAAHLAAGQVRAEEIEVAFLL
                     GNTAYNNYLLAISIGAVFFGANTYIGNGPNFMVKIIADQQKVKTPGFLGFIFKYTLPC
                     MGPMLLLIWLLFFRG"
     gene            complement(2182..2520)
                     /locus_tag="PKI07_06785"
     CDS             complement(2182..2520)
                     /locus_tag="PKI07_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50797.1"
                     /translation="MNANQQPVPTHFAPEVAYEVEPAPPAPFRARQATQLERLKARLL
                     AERLPPRPGLPLAASVQRAAREAAALAWTTRYPLLVFPELFDELAAAARQRVARQERI
                     FRRSRELLAT"
     gene            2858..3859
                     /locus_tag="PKI07_06790"
     CDS             2858..3859
                     /locus_tag="PKI07_06790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC50798.1"
                     /translation="MGDKPLKMVTCGGCQAKVFIPGDLPPLSSEPCKRCGYPIMMPMQ
                     LRQFELRSRIGGGGHGKVYRAFDTVLARLVAVKLMRQELEHDPEALASFYREARACAA
                     LNHTNIIHIYSFDEFEGQRYLVMELADRGSLDSRIDKQLRLPELEVLDIGYKMCSALD
                     MTLKHNLLHRDIKPGNILFNADGEPKLVDFGLARSPDAEPESAAVTEGTPYYVAPEKI
                     KRERETFLSDMYSLGCTLYHALTGHVPFEAPSVEELVAAHVTTAVTPPNQVLPEITQP
                     TSDALVKVLAKNPGDRYLSYDEFRMALEAARSQLLVQRYTQPPAGTGLKSKTSWWRR"
     gene            4070..4504
                     /locus_tag="PKI07_06795"
     CDS             4070..4504
                     /locus_tag="PKI07_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50799.1"
                     /translation="MKTSVLSRLFAGMLALALVGGSAASAADTKAKAPAASKPAAGKA
                     QTAKRDWYPFYGTVASVDKQARTISLQKKQGVRVLKLDAKSELIVNGQAATLADVKAG
                     NYAHGKLHKDAAGAEVITSAKFDKEAPPRKKAPAKAPATKTP"
     gene            4636..4923
                     /locus_tag="PKI07_06800"
     CDS             4636..4923
                     /locus_tag="PKI07_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOC50800.1"
                     /translation="MAKALTKSQTVASIAETVGITKKQASACLEALVALAYKQAKNSF
                     TLPGLGKLVLVHRKARMGRNPATGAQIKIPAKRVVKFRIAKAAKDAILGAK"
     gene            5102..5971
                     /gene="prmC"
                     /locus_tag="PKI07_06805"
     CDS             5102..5971
                     /gene="prmC"
                     /locus_tag="PKI07_06805"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOC50801.1"
                     /translation="MTVLEAIQRSAEFLAKKGVDSPRLQAELLLAHLLQLPRMRLYLS
                     FERVLAAGEVEALRGFITRRGRREPLQHIIGSVSFCGRELAVNRQALIPRPETELLAE
                     RGWMFLRQCAPVPPRPVTALDFGTGSGGVAIVLACQCPAAEITAVDISSAALALAREN
                     AARHGVAARLRLLPGDGFAALPPDAVFDLIISNPPYIPSGDIDRLQPEVRDYDPRAAL
                     DGGADGLDFGRRLAAEAARWLRPAGRLMVEFGDGQAERLRAIYAAQNWVVEEIVADYT
                     QRPRILIARRAGV"
     gene            5982..7244
                     /gene="murA"
                     /locus_tag="PKI07_06810"
     CDS             5982..7244
                     /gene="murA"
                     /locus_tag="PKI07_06810"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOC50802.1"
                     /translation="MDSLLIKGSVPLRGEVTISGAKNAVLPIMAATLLTTEPCVIRRV
                     PRLSDVQFMGQILTWLGAQVRSEGDTLRIQARKIQGAGDYDLIRKMRGSICVMGPLLG
                     RLKQASVSLPGGCVIGARPINLHLKGFEALGARVTIEGGYVHARAKRLAGATMFLGGR
                     AGSTVLGTANVMMAAVLAEGVTIIESAACEPEIVDLARFLTAMGAQISGAGSPTLTIT
                     GVKQLHGAEHEVIPDRIEAATYAIAAAATHGEVTLRGARAEHLRAVLDKLADAGVGIE
                     RRGADLTIRRPGRLKPVDITTLPYAGFPTDVQAQMMALMALTPGISIITERIFESRFM
                     HVSELARLGADIEIEGPSAIVKGGKPLSGAPVMASDLRASAALVIAGLAARGTTQINR
                     IYHLDRGYENIDGKLRQLGARIERIEES"
     gene            7241..7645
                     /locus_tag="PKI07_06815"
     CDS             7241..7645
                     /locus_tag="PKI07_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50803.1"
                     /translation="MTKLIAVLIIVGVIYGTWELFLYWEKVKNEEETRQKQDAAALVM
                     GDQLPGLPYDLQESLQVAQKKGAAGLEAWLKVHGQSVEDPRKAWIELDYCVAVARENP
                     AEARRVFARVKERLTASSPVWARMKQLEKTYQ"
     gene            7768..8286
                     /locus_tag="PKI07_06820"
     CDS             7768..8286
                     /locus_tag="PKI07_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50804.1"
                     /translation="MDVIFNCPKCEQELAVDSAGAGTEIPCPSCGEPLVIPEPELVSN
                     RPGLDSPAGAPRVEVNPINPIASSAAAKVQMNLRVPVRQTPTESLIEKPLPPLEVAAK
                     ETDKRIRIKTIKHTDCIEVGRDRFDEVVSDFLLKIGETNMISLTTIVYTHLDIGTQKL
                     LQDFGVLIVYRG"
     gene            8402..9409
                     /gene="bamD"
                     /locus_tag="PKI07_06825"
     CDS             8402..9409
                     /gene="bamD"
                     /locus_tag="PKI07_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOC50805.1"
                     /translation="MNSRSFRLVLVAVCLLAFPVRSPAPLIYRPGEGWIYEPYGGTGK
                     WQQSSAREQLEVAQAAFDKQGYGLALKASRYLVQKWPTSDYAPQAQHLLARCYDNLGR
                     TEKAFKEYQKLLEKQPKFADFTRIRQRQYEITDLYLAGKRFKLWRVFPYRSMERTAGM
                     YADIVKTGPYSDIAPQAQLKIGTAYEKIKALGFKAPDYPAAVQAYDLAADRYYDQPNV
                     AAEAIFRSGLASQKQALNAERDQTTAGRAIAKFTDFMALYPNDPRVAQAQETITALRG
                     EQARGSFVVAQFYEKRKRWESARIYYNEVLVQDPNSPYAAEARERIALLKPRLQGGAE
                     K"
     gene            9411..9923
                     /gene="lptE"
                     /locus_tag="PKI07_06830"
     CDS             9411..9923
                     /gene="lptE"
                     /locus_tag="PKI07_06830"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOC50806.1"
                     /translation="MRASCWLLVGLALLGGSGCAGYRLGPVNGLAPRGKSVEIQPFTN
                     QTLEPRLSDAVNAQLRKQLQRDGTYRLATHDDGDIIVSGSLIRYVRHEASFAAHDILT
                     VRDYRIELTAQVTARERSTGKVILDQPVTGKTLIRVTSDLSSSERQALPLLAADLARK
                     AVELLSEGGW"
     gene            complement(9967..10260)
                     /locus_tag="PKI07_06835"
     CDS             complement(9967..10260)
                     /locus_tag="PKI07_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50807.1"
                     /translation="MRRRINDEFADLPISRQRKYQLRMRRAGRCAECGEPAVQGSRCL
                     KHLVKARERQRRKRGLKRRYRGTLSYRLQSEARQKRLAGAGLSSPARSEPARL"
     gene            10467..10931
                     /locus_tag="PKI07_06840"
     CDS             10467..10931
                     /locus_tag="PKI07_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50808.1"
                     /translation="MRRAWIWFWLVGWLVAAGGAPAATGRVIKVLPQFLDLKGRNSLS
                     PSLYERDIYQARLRDHTNECSGMRFNVQWKTRGQPAAPLKLRAELRGVAHGDFPRQIT
                     LETPVQRRGWFSQWTWVALVGREYADFGRVTAWRVTLWEGDRLLGEQRSYLW"
     gene            complement(10962..11939)
                     /locus_tag="PKI07_06845"
     CDS             complement(10962..11939)
                     /locus_tag="PKI07_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC50809.1"
                     /translation="MSLGLMVFSLSGFGAGDDPPASPAAGAEEAKAQETLRAYLQLQE
                     QLHATELTLERNRKAAEEAAAETARGFEARLDEIQQTLMRQRAQELVALQSANRAMLF
                     VAGSFAALGLLAMAFIACFQWRAINRLAEFSAAWPAVPALGPGASRAALEAGEARVVA
                     EDPVGQSSQRLLGALERLEQRIRQLEHSSPPPLPDGGAPAPHAAPAPLQPNGAQPAPE
                     AAQITLLLGRGQSLLNLGQAAEALGCFEQALALEPNHAEALVRKGVALERLQKPDEAI
                     ACYDQAIAADASLTVAYLYKGGLFNRLERFNEALACYEQALRTQENRQR"
     gene            12364..12924
                     /locus_tag="PKI07_06850"
     CDS             12364..12924
                     /locus_tag="PKI07_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOC50810.1"
                     /translation="MAQKKTYLKGQLLLDSGQLRGSFFQRTVVLICQHDAEGAFGLVL
                     NRATGNNVGEMIVADLPDALKGCPLYLGGPVQPSALSYLHSDTFLAEANVMPNLSLGH
                     SMEDLVELGEGFSPTRKLKVFAGYAGWSPGQLEGEMKRKAWLTHPASLELVFDLAPEQ
                     LWAEILRRKGGLYRLLAQTPEDPSLN"
     gene            13046..13999
                     /locus_tag="PKI07_06855"
     CDS             13046..13999
                     /locus_tag="PKI07_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50811.1"
                     /translation="MRKSGNSRTATKTRPAPGRSGSSAGRIHPVVIYPIRPARDYADL
                     QALYQLVRYLDADKRRYARPLTVLDRKTHYALRGDKEYLDFRRLVVSRSSEVLDVWAV
                     DTCQMWYAGLGAAFERGAEHDVYWLIPGDFNYGTKMGHQVLGRLHDLPEIVRELNQDL
                     CIGEITADYTNSKQLIDTYGTFALLYNWFPDEAREIRQYTERPRSEFFAVRHDFLGKA
                     LCQRWYPYEQTMVMLLQAVRGHRGISRFSVGNISDLPEGKESLAAAMQQVERTERVLK
                     SVWREYHQGDPDWIERYRQLVGTSTQVRYTAISILRNLLEE"
     gene            13999..14283
                     /locus_tag="PKI07_06860"
     CDS             13999..14283
                     /locus_tag="PKI07_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOC50812.1"
                     /translation="MRAFAYQKPLRAVNFICHAPEARAVSLVGDFNGWDPAALPMKQM
                     PDRSWLLTVELKHGHHRYAFLVDGVLTLDPHAQGITRNEKGERVCLIPVS"
     gene            14377..16284
                     /locus_tag="PKI07_06865"
     CDS             14377..16284
                     /locus_tag="PKI07_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOC50813.1"
                     /translation="MSVLTMTAAVPNPAPAEERGARLDPALVQYFRLETARLSERCLA
                     DIHSLADWEARRGEYRRQLQEMLGLWPAPERTELRPVITGKLEQLDFTVEKLHFQALP
                     GLYVTANLYRPWKVEDPLPAILYLCGHSRVMTNGVSCGNKAGYQHHGAWFARNGYVCL
                     VIDTVQLGEIPGQHHGTYSDGQWWWNARGYTPAGVEAWFGIRALDYLCSRPEVDKERI
                     GLTGRSGGGSYTWTVAALDERVKVAAPVAGITDLRNQVVDGCVEGHCDCMFYVNTYRW
                     DFPQAAALLAPRPLLIGNTDKDQIFPLDGVVRLYNQVRRIYALYGKTNHLGLLITEGP
                     HADTQDLQVPVFRWFNRFLKGQTPPIEMAAVKFFAPEQLRVFDSLPADEKNTTIPESF
                     VPRAAKSPPPAEYRKALREKVFGGWPAESGTPPLELRERADRAGLRVGVYVLESQPAV
                     KLPLWVLQAPKTRRPEQVLLTVLDAERWTHSPAKGLWRGGEAPETDAHLRQEIQAGKL
                     ALAFFAPREVEPASWQTDPKLARQVRRRYMLLGQTLDGMRVWDIRRAVQALKTLPEFK
                     SAALGINARQQMGVNAAYAALFEPEITQLQLEGLPASHQEGPDYLNVLKVGDIPQVLA
                     LLGPRVAAPAR"
     assembly_gap    16686..16785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16786..18332)
                     /locus_tag="PKI07_06870"
     CDS             complement(<16786..18332)
                     /locus_tag="PKI07_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HOC50814.1"
                     /translation="MGYDLFLSYSRGDNAQGRITELLRHIQADFQKHAQRELRVFFDQ
                     EEIRGMEDWRLRILQGLRDSHLLLACLSPGYLASQNCKWEFNEYLKHEIGRAGGSHGV
                     VPVYLVEVPSWEQKGDKQQNEAWADELRRRQYFDLRLWYDQGAAALLEAAVQERMAEL
                     SHRIWDAIQHGERAARSPGNVEAHNPHFVGRRRPLRQLRQNFVEPGRISVLTAVHGMG
                     GLGKTALATEYAHAYAEEYGGGRWLVRCAGREDLRLALAELAPAVGFEFTEDEKKSAD
                     RLFERVRRELQQLAAGGNPPRCLLILDNVDQPGLLDPAMVARLNGGDWLHVLATTRLG
                     ENELGGTHRDRAFLPLDELPGDEALALMESYQPGGAISPRDGAGGRRRDSAAAGELHP
                     GGGSGGGVSGAVWGGGQLRGVSGAAGAGGVGGAGSGGEAGGGSGAAWGGVFKRDAGAD
                     AGAAGGGGTAGVAPGGVAAVRPGGVAVAARAGGQDVSGVGAGCGAGLSRSVEPCGAAA
                     AEPAIAAG"
     gene            19605..21410
                     /gene="ilvB"
                     /locus_tag="PKI07_06875"
     CDS             19605..21410
                     /gene="ilvB"
                     /locus_tag="PKI07_06875"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOC50815.1"
                     /translation="MSGSEILVACLEREGVDTIFAYPGGASMEIHQALTRCKTIRTVL
                     PRHEQGGAFAAGGYGRVTGRAGVCMATSGPGATNLVTGIADAYMDSVPLVAITGQVVQ
                     AMIGKGAFQETDIFGMTLPVVKHSYLITDINDIPRIVKEAFYLAQSGRPGPVLIDLPK
                     NVQQARTQPVFPKETSLRSYRADPRASELALNEIIGLIEKSERPVLYVGGGIISGNAS
                     KELRQFAEATQIPVTTTLMGCGAFPETHPLSLRWLGMHGAAFANWAVNGEYHPAARPT
                     EPAKLKAAGADLLLAFGVRFDDRVTGRVDKFCEKGTIVHLDIDPSEINKNKPVSLPII
                     SDVKFALGRLAAMVKARPLQKKFTAWHKQIARWKARAPFGYSVTEEVMKSQHMRDHLK
                     GREREVILPQMAIEMLYELTRGEAIITTGVGQHQMWAAQFYQFKYPRQFVTSAGLGAM
                     GFGYPAALGAKVAFPNRQVVDIDGDGSFLMNIQELATAHIEKIAAKVIVLNNQHLGMV
                     MQWEDRFYAGNRGNTYLGDPERRKQIYPDYTAMCQSFGVKCERVMYLKDLRPALQRML
                     DANEPYVLDVIVPYTEHVLPFIPSGRTVADMIWQA"
     gene            complement(21552..21908)
                     /locus_tag="PKI07_06880"
     CDS             complement(21552..21908)
                     /locus_tag="PKI07_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50816.1"
                     /translation="MKTPFCLLLAVSGLLAGCGEKAGAPAPPANSATPGSSPLSAPAD
                     YLGALDKAHKTADKVVDTAVLNQAIQLFNVEHGRNPRDLNELVEEKFIPKLPTAPYGM
                     KLDYDAATGKVRVVKQ"
     gene            22087..22506
                     /locus_tag="PKI07_06885"
     CDS             22087..22506
                     /locus_tag="PKI07_06885"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOC50817.1"
                     /translation="MAVEINITYHGGLRCAATHEPSGQTLSTDAPLDNGGKGEAFSPT
                     DLVATALGTCMATIMGRVADQNNLNLDGLQIRVLKEMATDPVRRIRALKTRLVFPRAA
                     ALSAADRAKLEAAARACPVKQSLHPEVQLPIEFVYPA"
     gene            complement(22597..24012)
                     /locus_tag="PKI07_06890"
     CDS             complement(22597..24012)
                     /locus_tag="PKI07_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50818.1"
                     /translation="MVATALQTNSFTRRQFLRRTALAAGAMTLAFPSVSRVLGANSRI
                     NIACIGVGGKGDSDVSDAINCGGQIVALCDVDQKTLDGKAKKVAEKFPQLKRYRDYRK
                     LLDELGKDIDAVTISTPDHQHGVAAIRAMKMGKHCFCQKPLVQTVSEARQVRQLAKDK
                     NLATQMGNQGSAESGLRRAVEIVQAGVIGKPLELHVWSNRPIWPQGLDRPAGQDPVPP
                     ELDWDLWLGPARKRPYKKGAYHPFAWRGWYDFGTGALGDMACHTVNMPFRALNLGYPN
                     VVECELASRLYPEAFPKTSRIRFEFPQRGDLPPLKFWWYDGKPADPLKPLRPQGDAIH
                     EIIATYDKLPASGALIIGEKGKVFSPDDYGARFFVAMKGSSEYVASDQHEACKAVPQS
                     IPRSPGHMEEWFRMMKDGVPAYSNFDIAAYLTEIILLGCVALRVGEGYRMEWDGPNMR
                     SPNLPAAAQFVKRDNRRGWNA"
     gene            24465..>27021
                     /locus_tag="PKI07_06895"
     CDS             24465..>27021
                     /locus_tag="PKI07_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC50819.1"
                     /translation="MESPQTRCLRTAIFAALLLVLGTACPAAAPEYRAFWVDAWNAGL
                     LSQSQVDTLLGVPGNANSKGVIREANCNMVIVQVRRRADVCYPSGVGEPYMSGLSPAN
                     FNSLAAMIKAAHDTTGGKKRVEVHCWSVVFKTAKGQVYSQHSDPPTGSLTTFDNYWPT
                     RLSSTTGAENSDGAFDPGHPKCLEYLINAHMDLVNFQTTAGPGGADGRIDGIHYDYIR
                     FEAATEGFNPTSVARYNARYGLSGNPSAASEQFKQWRRDQVTAFARQMYARIQKTSPQ
                     IRQSGSFVTWNPSPASSTRAAFQATRPYYDVYSDWDSWQQEGIMDMAVPMTYYNWASL
                     PSDYTKWMNFEKDRKFNRHMIIGPGIYLNSLANAIYELQMTRDASPAGNYAHGFSGYS
                     YRTPYSGGTWAGFAPSLVSSVTPTWADIPEMPWKTSPTKAHIMGTVTIHGSGAWADGA
                     RVSLSGPVSRVQTNDGTGFYAFIDLPVGTYTVTASLAGHPTATGSTTIAVGQVTGNMY
                     ELDLVLGGNVPPTITTQPISQSVKQGTNATFTVGVSGTAPFAYQWRLNGADISGATAS
                     SYTRANVQPADAGTYSVVVTNAYGMATSSGALLTVVVPPTITTQPLSQTVTQGQNATF
                     TVAASGTAPFTYQWRFNGAPIGGATASSYTRSNVQLSDAGDYSVVVANSAGSDTSAKA
                     VLTVVVPPVPPAITTQPQGQTVVLGETAAFNVVATGGIPLSYQWRFNGADIAGATDSS
                     YARAQVGAADAGDYSVRVTNPYGSLLSDSATLVVDTNVTLPVIVTQPQSQTVIAGQSA
                     TFSVLATNKAPLSYQWRLNGAPIAGATGSSYTRSNVQTGDAGNYSVVITNIIGA"
BASE COUNT         4767 a         8407 c         9067 g         4680 t
ORIGIN      
        1 caccttgagt ggaaaccatg acgaccaatt tgctgaactt gacggacaca actcatagcc
       61 gttcaggtcg tagccttctc agtcctattg actggcgatt ttcacgatat aaccatcaaa
      121 tccactttgg ctttcgactg taaaatcagc gatttacgat ttcgatgagg gttgaaatcg
      181 gggtgggatc caaattattc cggcctgggc gtgccttcat tcagatgcct tggtttttcc
      241 ctgcccctac gccgtgtaca aggggtgtac aagggatgcc catcggatgt accagctaaa
      301 atcctatgtt attccggtga gcatcccttg tacacccctt gtacacggcc aaggattagg
      361 gtagcgcggt caatatgtgc ctgaatctaa gcgatttatg ggctattttc ctgccgttct
      421 ttcctgaatg tctaaaccat tgattttaca gtggaaaggc attgggagct caatggccag
      481 gccgttaaat gcgccaatta aagggtttat tggggctgtg ggccgggcgg ccgggtttct
      541 tcaggctcca gtggcagggg agcgcgtggg ttggctgcgc cacagaatcg ctccggctga
      601 gggtggcgtt tccggggcgg ttttatttgc cgtttgcgat gggggcgggg ggcttcagcc
      661 gcggaagaac agcagccaga tcaggagcag catgggcccc atgcagggga gggtgtattt
      721 gaagatgaaa ccgaggaatc caggggtttt gaccttctgc tggtcggcga tgattttgac
      781 catgaaattg gggccgttgc cgatgtaggt gttggcgccg aagaacaccg cgccgatgct
      841 gatggcgagg agatagttgt tataggcggt attgcccagc agaaacgcca cttcgatttc
      901 ctccgcccgc acctgcccgg cggccaggtg tgccgcatgg tatttttgca gcgccgcgta
      961 agtgtttccg atggcctcgg cctgcggccc ggtgagggcg gcgaggcccg cgccggattg
     1021 ctggacgagt tgttggacct tgtcaatgat ttcggggtcc acgaacaggc cgaaggcggc
     1081 gctcaggaag ctgaggtacg tcggcgcatt atcaagcaag ctggacagga tgccgctgct
     1141 ccagtagaag acggccgggg cgggggcgcc aatccggctg gcattgtgtt gcagccagtc
     1201 cagggccggc atcatggtgg cgaagatgcc gatgaacagg atggcgactt cctggatggg
     1261 gcggaaatcg aagtggttgg cggcatggat gcgcgggcgg gtggtgagcc aggagccggc
     1321 ggccgcgccc agcatcaggg cttcgcgcag gaacaacggg cgggagatga acacggccac
     1381 gaggatgacc agcagatagg ccaggttgcc gaggccctcg cagcgccatt ggtcgggcgg
     1441 ttcggccagg cgttcgcgca cggcccgggg ggcgcgccgg tagttgcggg cgtccaggac
     1501 ataaaacagc gccagcagga agcccacgcc caccgcccag atcggccagc aatgctcagc
     1561 cacccaccag aacggaatgc ctttgaggta gcccaggaac aggggcgggt cgccgatggg
     1621 ggtgaggcag ccgcccacgt tggaaacgat gaagatgaaa aagacgatgt gatgcgcggt
     1681 gatgcggtat ttattcatcc ggatccaggg ccggatgagc agcatggagg cgccggtggt
     1741 tccgaacacg ttggccagca aggcgcccag gagcagaaag agggtgttgg cccgcggcgt
     1801 cgcctcgccg cggacgtgga ggtggatgcc gccggagacg acatacagcg agccgatcag
     1861 gacgatgaag ctgacatatt cgccgcctcc cagcagcacg cgctcatacg cgcgcagcac
     1921 gaacaggtag taggcgagcg tggtcgcgcc gagcgacagg gccactttgg gataatgccg
     1981 gctccaccaa tccgggcaga gcagcggggc cagggcaatg gttcccagca gcacaacaaa
     2041 aggcaaaacc atccatggat tgggcgatgc gaacggatga tccattccgc ttaccctacc
     2101 ggggcgggca aaatcgggtc aaacagattc ccgcgccgcg gggcggaacg cccggggcgg
     2161 cgcgggttaa acgcgcgggg ttcatgtggc cagcagttcg cggctgcggc ggaagatgcg
     2221 ctcctgccgc gcgacgcgct gccgcgcggc ggcggccagc tcgtcaaaca actccggaaa
     2281 caccagcagc gggtagcggg tggtccaggc cagcgccgcg gcctcgcggg ccgcgcgctg
     2341 caccgaggcg gccagcggca ggcccgggcg cggcggcagc cgttcggcga gcagccgggc
     2401 tttcagccgt tccaattggg tggcctgccg cgcccggaac ggcgccgggg gggcgggctc
     2461 cacttcatac gccacttcgg gggcgaagtg ggttggcacg ggttgttggt ttgcgttcat
     2521 atcggtttat cttttgctaa cggccgcccg tttcagcggc ccgggggcaa taaaaaaccc
     2581 gcgattgacg ggcaatcgcg ggtttcagga aaatgcaaac aatggatcag ccagttcctc
     2641 ccgcgatgcc gggaccgcga tgcgcgatgg gcgtttgccc accgactcgc cggccacatt
     2701 gacaaatgac aaacaggttc atcatttacg attgagcgca acctaacccg gcgcgggcgg
     2761 tttgtcaaat ggtcgcgggg cgttcgcgtt tttggcgggc gggggcggcg gggcttgtct
     2821 cgcgttccgg gggtgctatg atgggcggat aataagtatg ggcgataaac cattgaagat
     2881 ggtcacgtgc ggcggatgcc aggccaaggt ctttattccg ggcgacctgc cgccgctttc
     2941 ttccgagcca tgcaaacggt gcggttatcc gatcatgatg ccgatgcagt tgcggcagtt
     3001 cgagttgcgc agccggattg gcgggggcgg gcacgggaag gtttatcggg cgttcgacac
     3061 ggtcctggcc cgcctggtgg cggtcaagct gatgcggcag gaactggagc acgacccgga
     3121 ggcgctggcg agtttctacc gggaagcgcg cgcctgcgcg gcgctgaacc ataccaatat
     3181 cattcacatc tactcgttcg atgagtttga ggggcagcgc tatctcgtga tggagctggc
     3241 ggaccggggc agcctggatt cccgcattga caagcaactg cgcctgccgg agctcgaggt
     3301 gctggatatt ggctacaaga tgtgttccgc cctggatatg acgctcaagc ataacctgct
     3361 ccaccgcgac atcaagccgg ggaatatcct ctttaacgcg gatggcgagc cgaagctggt
     3421 ggatttcggc ctggcgcgca gtcccgatgc cgaacccgag tccgccgcgg tcacggaagg
     3481 gacgccctac tacgtggcgc cggagaagat caagcgcgag cgggagacgt ttttgtcgga
     3541 tatgtacagc ctgggctgca cgctttatca tgcgttgacc ggccacgtgc cgttcgaggc
     3601 gccgagcgtg gaggagctgg tggccgcgca cgtcaccacg gcggtgaccc cgcccaacca
     3661 ggtgctgccg gaaattaccc agcccacaag cgatgcgctg gtgaaggtgc tggccaagaa
     3721 cccgggggat cgctacctca gctacgacga gttccggatg gcgctggagg cggcgcgcag
     3781 ccagttgctg gtgcagcgct acacccagcc gccggcgggc acgggcctta aaagcaagac
     3841 cagttggtgg cggcggtagg gttgggccgc cccgccgccc gcggcgaggc cggcgccgcc
     3901 gccggtgagg acgcggccgc ggggccggtg ggggattttt tcccggcggg cttgattaat
     3961 ggccgcgggc tgctaggttg tcggctgcgc ggcgggccgg cgtgggtgcg ccgggcgggc
     4021 gcgcacgaaa acagtctcca atagaataaa gaacgaatcc atgcacgtta tgaaaacctc
     4081 cgtattgtcc cggttattcg cggggatgct cgcgctggcc ctggtgggcg gctcggcggc
     4141 ctccgcggcc gataccaaag ccaaggcgcc cgcggcgtcc aaacccgcgg cgggcaaagc
     4201 gcaaaccgcc aaacgcgact ggtatccttt ttacggcacg gttgcctcgg tggataagca
     4261 ggcccggacc atttccttgc agaagaagca aggggttcgg gtgttgaaac tggatgcgaa
     4321 gtctgagctc atcgtcaacg gccaggccgc cactctcgcc gacgtcaagg cgggcaacta
     4381 tgcgcacggg aaactccaca aggacgcggc gggcgccgaa gttattacgt ccgccaagtt
     4441 cgacaaggaa gcgcccccga ggaaaaaggc tccggcgaaa gcgcccgcca caaagactcc
     4501 gtaacgaaac gccaactccc cgaggccgcg cgcggcacct ttccccggcg ccggccccgg
     4561 cgttgcgggc cggggcggcg ggcgcatttc gggcttgcgt ttatggcgcg gggctgttaa
     4621 ccaagcggca catatatggc aaaagcatta accaaatcac aaactgtcgc atctattgcg
     4681 gaaaccgttg gcattaccaa gaagcaggcg agcgcctgcc tggaagcgct ggtggcgctg
     4741 gcctacaagc aggcgaagaa cagctttacg ctgccgggcc tgggcaagct ggtgctcgtc
     4801 catcgcaaag cccgcatggg ccgcaacccg gccacgggcg cgcaaatcaa gatcccggcc
     4861 aagcgggtgg tcaagttccg catcgccaag gccgccaagg atgcgatcct gggcgccaag
     4921 taagcggagc tgaattcttt gaatgatcgc ggcggaggaa tggcctccgc cgttttattt
     4981 ggctcccggg ccgggcgggc acggcccgcc ggggcgcggc gcgcgcgggc ggggatggcg
     5041 tggccgggcg cggcggcgtt ttgggctttg cgcgcggccc cgggggcgta gcatcgcccg
     5101 tgtgacggtc ctggaagcga tccaacgcag tgcggagttt ctggcgaaga aaggggtgga
     5161 ctcgccgcgc ctgcaggcgg agctgctgct ggcgcatctg ctccagttgc cgcgcatgcg
     5221 gttgtacctg agtttcgagc gcgtgctggc ggcgggcgag gtggaggcgt tgcgggggtt
     5281 catcacgcgg cgcggccggc gcgagccgtt gcagcatatc atcgggtccg tctcgttctg
     5341 cggccgggag ctggcggtga accggcaggc gctgattccg cggccggaaa cggagctgct
     5401 ggcggagcgc ggctggatgt ttctccgcca gtgcgcgccg gtcccgcccc gccccgtcac
     5461 cgcgctggat tttggcaccg ggagcggggg ggtggcgatt gtcctggcct gccagtgtcc
     5521 ggcggcggag atcacggcgg tggatatttc gtccgcggcc ctggcgctgg cccgcgaaaa
     5581 cgccgcccgc cacggcgtcg cggcgcgcct gcggttgctc ccgggggatg gctttgcggc
     5641 gttgccgccg gacgcggtct ttgatctgat catcagcaat ccgccgtata ttcccagcgg
     5701 ggacattgac cggttgcagc cggaagtgcg cgactacgat ccgcgggccg cgctggacgg
     5761 gggcgccgat gggctggatt tcgggcgccg cctggcggcg gaagcggcgc gctggctccg
     5821 gccggcgggg cggctgatgg tggagtttgg cgacggccag gcggagcggc tgcgcgcgat
     5881 ttacgcggcg cagaactggg ttgtggaaga gatcgtggcg gactacactc agcggccaag
     5941 aattctgatc gcccggcgag cgggtgtatg aggttgatgg catggacagt cttttgatta
     6001 aaggttcggt gccgctgcgt ggggaagtca ccatcagcgg cgccaagaat gccgtgctgc
     6061 cgattatggc ggccacgctg ctcacgactg agccgtgcgt gatccggcgg gttccgcgtt
     6121 tgagcgacgt gcagttcatg ggccagatcc tcacctggct gggcgcccag gtgcgcagcg
     6181 agggcgatac cctccgcatc caggcccgga agatccaggg cgcgggcgat tacgatttga
     6241 tccggaagat gcgcggctcg atttgcgtca tggggccgct gctggggcgc ttgaagcagg
     6301 ccagcgtctc gctgccgggc gggtgtgtga tcggcgcccg gcccatcaac ctgcacctga
     6361 aaggcttcga ggcgctcggc gcccgggtca ccatcgaggg cgggtatgtg catgcccggg
     6421 ccaaacggct ggccggcgcc acgatgttcc tgggcgggcg cgccggctcg acggtgctcg
     6481 gcacggcgaa cgtgatgatg gcggccgtcc tggcggaagg cgtgaccatc atcgaaagcg
     6541 ccgcctgcga gccggaaatc gtggacctgg cccgcttcct caccgcgatg ggcgcgcaga
     6601 tttccggcgc gggcagccct acgttgacca ttaccggcgt gaagcagttg cacggggccg
     6661 agcacgaagt catccccgac cgcatcgagg ccgccactta cgccatcgcc gccgccgcca
     6721 cccacggcga ggtcaccttg cgcggggcgc gcgccgaaca cctgcgcgcc gtgcttgaca
     6781 agctggcgga tgccggcgtg ggcatcgagc gccgcggcgc ggacctcacc atccgccgcc
     6841 ccggccgcct gaaaccggtg gacattacca cgctgcctta tgcgggattt ccgacggatg
     6901 tccaggcgca gatgatggcg ctcatggcgc tgacgccggg catcagcatc attacggagc
     6961 ggattttcga gtcccggttc atgcacgtca gcgagctggc gcgcctgggc gcggatattg
     7021 aaatcgaggg ccccagtgcg attgtgaagg ggggcaagcc gttgagcggc gcgccggtca
     7081 tggccagtga tttgcgggct tcggccgcgc tggtcatcgc cgggctggcc gcccgcggaa
     7141 ccacccagat caaccgcatt tatcacctgg accgcggtta cgaaaacatt gatggcaagc
     7201 tgcgccagtt gggcgcgcga attgagcgaa tcgaagaatc atgacgaagc tgattgcagt
     7261 gctgattatc gtgggggtca tttatggcac ttgggaactg ttcctgtact gggagaaggt
     7321 caagaacgag gaggaaaccc ggcaaaagca ggacgccgcg gccctggtga tgggcgatca
     7381 attgccgggc ctgccctatg acctccagga aagcctgcag gtggcgcaga aaaaaggcgc
     7441 ggcggggctg gaggcctggc tgaaggttca cggccagagc gtcgaggatc cgcgcaaggc
     7501 ctggatcgag ctggattatt gcgtggcggt ggcccgggaa aacccggcgg aagcccggcg
     7561 cgtctttgcc cgcgtcaaag aacggcttac cgcgtcctcc cccgtctggg cgcggatgaa
     7621 gcagctcgaa aagacttacc agtaggcggc cggagcgccg gcggtccggg gttgggcggg
     7681 gcggtttgag gcggtgtttt taggcttgcc cttttcccgg ggatgcctat cgtacggctg
     7741 gacccagtga acgaaaggcc agacgttatg gacgtaatat ttaattgccc gaaatgcgaa
     7801 caggagttgg ccgttgacag cgcgggagcc gggacggaaa tcccctgccc ctcctgcggg
     7861 gagcccctgg tgattccgga gccggagctg gtgtccaacc ggcccgggct ggacagcccg
     7921 gcgggcgcgc cgcgggtcga ggtaaacccg attaatccca tcgcctcgtc cgccgccgcc
     7981 aaggtgcaaa tgaacttgcg cgtgccggtg cgccagacgc ccaccgagag cttgatcgaa
     8041 aaaccgcttc cgcccctgga ggttgccgcc aaggaaaccg acaagcgcat ccgcatcaag
     8101 accatcaagc acaccgattg catcgaggtg gggcgcgacc ggtttgatga ggtggtgtcg
     8161 gactttttgc tcaagatcgg cgagacgaac atgatcagcc tgacgacgat cgtctatacc
     8221 cacctggaca tcggcacgca aaagctgctc caggacttcg gggtgctgat cgtgtaccgg
     8281 ggctagccgg cgttccgggg cggctccgtc cggtctcgcc gggattgcgg cctccgcgcg
     8341 gctcgggggg cgggctgcgg gctgccttga ctccccgggc gcgcattgtt agcgtggcgc
     8401 catgaatagc cggtcttttc gtctggtgct ggtggcagtc tgcctgctgg ctttccctgt
     8461 tcgctcgccc gcgcccttga tttatcgccc gggcgaaggc tggatttacg aaccctacgg
     8521 cggcacgggc aagtggcagc agtcgtcggc ccgggagcaa ttggaagtgg cccaggccgc
     8581 gttcgacaaa cagggctacg gcctcgcgtt gaaagcctcg cgctacctgg ttcagaagtg
     8641 gccgacgtcc gattacgctc cccaggcgca gcacctgctg gcccgctgtt atgacaacct
     8701 cgggcggacg gaaaaggcgt tcaaggaata ccagaagctc ctggaaaaac agccgaagtt
     8761 tgcggatttc acccggatcc ggcagcgcca atacgagatt accgaccttt accttgccgg
     8821 caaacggttc aagctctggc gggtgtttcc ctaccggtcc atggagcgca cggcgggcat
     8881 gtatgctgac atcgtgaaaa ccggcccgta cagtgacatt gctccccagg cgcaattaaa
     8941 gatcggcacg gcctacgaaa agatcaaagc cctcgggttc aaggcgccgg attatcccgc
     9001 cgccgtccag gcatacgacc tggcggcgga ccgttattat gaccagccga acgtggcggc
     9061 ggaggccatc ttccgttccg gcctggccag ccagaaacag gcgctcaatg ccgagcgcga
     9121 tcaaaccacc gccgggcggg cgattgccaa attcacggat ttcatggcgc tctaccccaa
     9181 cgacccgcgc gtggcccagg cgcaggagac catcaccgcc ctccggggcg agcaggcgcg
     9241 cggcagcttc gtcgtggcgc agttctatga gaagcgcaag cggtgggaaa gcgcgcggat
     9301 ttactacaac gaggtgttgg tgcaggatcc caactcgccg tacgcggcgg aggcgcggga
     9361 gcggattgcc ctgctgaagc cgcggctcca gggcggggcg gaaaaataac atgcgcgcct
     9421 cttgctggct gctggttggc ctggccctgc tcggtggaag cgggtgcgcg ggataccggc
     9481 tgggccccgt caatgggctg gccccgcgcg ggaagtcggt ggaaatccaa cccttcacca
     9541 accagacgct cgagccgcgc ctgagcgatg cggtcaacgc gcagttgcgc aagcagttgc
     9601 agcgcgacgg cacctaccgg ctggccaccc acgatgacgg ggacatcatc gtgagcgggt
     9661 cgctgatccg ctacgtccgg catgaagcca gctttgcggc gcacgatatt ctcacggtgc
     9721 gggactaccg gatcgaactc accgcgcagg tcaccgcgcg cgagcgcagc accggcaaag
     9781 tcatcctcga ccagccggtc acgggcaaga cgctgattcg cgtcacgagc gatttaagca
     9841 gcagcgagcg ccaggcgctg cccttgctgg ccgccgacct cgcccgcaag gcggtggagt
     9901 tgctgtccga aggcggctgg tagcccggcg cgggccccgg cgggaattcc cgttttcagc
     9961 gggcgtttat aagcgggcgg gctccgaccg ggccggggag gacagcccgg cccccgccaa
    10021 acgcttctgg cgggcctcgg attgcagccg gtagctcagg gtgccgcggt agcggcgttt
    10081 caggccccgc ttgcggcgct ggcgctcccg cgctttgacc aggtgcttga ggcaccggga
    10141 gccctgcacc gcgggctcgc cgcactccgc gcagcggccc gcgcgccgca ttcgcagttg
    10201 gtatttccgt tgccgcgaaa tcggcagatc cgcaaactcg tcgttaattc gtcttctcat
    10261 ggcagcccgt tccttcccgt caaacaatgc ccgttatggc ttgagcccgg tttcggggat
    10321 tggcctatcc ccattctacc gcatttctcc cgcgttgtca tgcgcggaaa gcggcgcgca
    10381 ccgccgggac ggcgcgcggg cggcggcggg gggcggcggc gaaaatgcct gttgagttcg
    10441 gggcgcgggg cggctatttg ttgggtatgc gacgggcgtg gatctggttt tggctggtcg
    10501 gatggctggt ggcggcgggc ggcgcgccgg cggccaccgg gcgcgtgatc aaagtgctgc
    10561 cgcagttcct cgacctgaag ggccgcaact cgctttctcc cagcctttac gagcgcgaca
    10621 tctaccaggc ccgcctgcgc gaccacacca acgaatgctc cgggatgcga ttcaacgtgc
    10681 aatggaagac ccggggccag ccggcggcgc cgctcaagct gcgggccgag ctgcgcggcg
    10741 tcgcccacgg cgatttcccg cggcagataa cgctggaaac gccggtgcaa cggcgcgggt
    10801 ggttcagcca gtggacgtgg gttgccctgg tcggcaggga atatgcggac ttcggcaggg
    10861 ttacggcctg gcgggtgacg ctgtgggagg gcgaccggct gctgggcgaa cagcgttcgt
    10921 acctctggta ggcgcccggg ctgcgggcgg ctccggccgg gttaacgctg ccggttttcc
    10981 tgggtgcgca gggcttgctc gtagcaggcc agcgcctcgt tgaagcgctc caggcgattg
    11041 aaaagccccc ccttgtaaag atacgcaacg gtcagggaag catcggcagc gatggcctgg
    11101 tcgtagcagg cgatggcttc atccggcttc tgcagccgct ccagcgccac gcccttgcgg
    11161 accagggctt ccgcgtggtt cggttccagc gccagagcct gctcgaagca gcccagcgct
    11221 tcggcggcct ggccaaggtt taacagggat tgcccccggc ccaacagcag ggtgatctgc
    11281 gccgcttccg gagccggctg cgcgccgttg ggctggagcg gggcaggggc ggcatggggc
    11341 gccggagcgc cgccatccgg cagcggcggc ggggaggaat gttctaattg gcggatgcgt
    11401 tgttcgaggc gctccagggc gccgagcagc cgctggctgg actgtcccac cgggtcttcg
    11461 gcgaccacgc gcgcctcgcc cgcctccagc gccgcccggg atgcgcccgg cccgagcgcc
    11521 gggacggcgg gccacgccgc ggagaactcc gccagccggt taatggcgcg ccattggaag
    11581 caggcgatga atgccatggc caggagcccc agggcggcaa acgagccggc cacgaacagc
    11641 atcgcccggt tggcgctttg cagggccaca agctcctgcg cgcgctgccg catcaaggtt
    11701 tgctggattt cgtccagccg ggcttcgaac ccgcgggcgg tttccgccgc cgcttcctcc
    11761 gcggctttgc gattgcgttc gagggtcagt tcggtggcgt gaagctgctc ctgaagctgc
    11821 aagtacgcgc gcagcgtttc ctgtgctttg gcctcctcgg cgcccgccgc cgggctggcc
    11881 ggcgggtcat caccggcgcc gaaacctgac agggagaaaa ccatcagccc caggctcaca
    11941 aggatagcgc gcgcccaggc ggccagccga tcgcccgcca gcgccgcgcc gccgcctgag
    12001 gccggccgcc ggaaggaaat gccgcttgca acagcagggg ctatgactgg tattcggctc
    12061 accgcgcctc cagaatggcg caagccgccg gccatgtcaa accgggtttc gacgccggaa
    12121 acgcgcccgc cgccgggaga atctcagggg cgcggcggcc gccaacccct ggcgggcggc
    12181 ggcggaaatc agtcgttcgg aacccagcct ttgcccggga tgaaatcccg gctgccggaa
    12241 tgagtccggc agcccgtggc cacaagcagg gcgacggcca gcatcaacaa cagacaaagc
    12301 tttgacatgg ggacaattta atccagatta agagcagcgc aagcgataga catcctgggc
    12361 gcgatggcgc agaagaaaac atatttgaaa gggcagttgc tcctggacag cggccagttg
    12421 cggggctcgt tcttccagcg gacggtggtg ctgatttgtc agcacgacgc cgagggcgcc
    12481 ttcggcttgg tgctcaaccg cgccacgggc aacaacgtcg gcgaaatgat cgtcgccgac
    12541 ctgccggacg cgctcaaagg atgcccgctc tacctgggcg gcccggtcca gccgtcggcc
    12601 ttgagttatc tgcactccga taccttcctc gccgaggcca atgtcatgcc gaacctgagc
    12661 ctgggacatt cgatggagga tttggtggag ttgggcgagg ggttttcgcc gacgcgcaag
    12721 ctcaaggtgt ttgcggggta tgcgggttgg agccccggcc agctcgaggg cgaaatgaag
    12781 cgcaaggcgt ggctgacgca tccggcgtcg ctcgaactgg tattcgacct ggcgcccgag
    12841 cagctctggg cggaaatcct gcggcggaag ggcggcttat accggttgct ggcgcagacg
    12901 cccgaggacc cgtcgctcaa ttgagaacgc cttgcccggg agcgccagag ccggggaacc
    12961 tgcgctttga acgttgcccg cgttgcggcg cggggatagg gtggttcggc gcgggcccgc
    13021 cacgcgcggg ccggagagca ttgttatgag gaaaagtggc aacagtcgga cggcaactaa
    13081 aaccaggccg gcgccggggc gttccggttc cagcgccggg cggattcacc cggtcgtcat
    13141 ttacccgatt cggcccgcgc gcgattacgc cgacctgcag gccctgtatc aactggtccg
    13201 gtacctggac gcggacaagc gccgctacgc ccggcccctc acggtgctcg atcgcaagac
    13261 gcactacgcc ctgcgcggcg acaaggaata cctggacttc cggcggctcg tcgtttcgcg
    13321 gagttcggaa gtgctggacg tctgggcggt ggatacctgc cagatgtggt atgcgggcct
    13381 gggcgcggct tttgagcggg gcgcggagca cgacgtttac tggctgatcc ccggcgattt
    13441 caactacggc acgaagatgg ggcaccaggt cctcggccgg ttgcatgatt tgccggaaat
    13501 cgtccgcgaa ctgaaccagg acctgtgcat cggcgaaatc accgccgact acaccaactc
    13561 gaagcagctc attgatacgt acggcacctt tgccctgctc tacaactggt tcccggacga
    13621 agcccgggaa atccggcaat acaccgaacg gccccgctcg gagttcttcg ccgtgcgcca
    13681 cgacttcctg ggaaaggcgc tgtgccagcg gtggtatccc tacgagcaga ccatggtcat
    13741 gctcttgcag gcggtccgcg gccatcgggg catcagccgg ttctcggtgg gcaacatctc
    13801 ggacctgccg gagggaaagg agtcgctggc cgcggcgatg cagcaggtcg agcggacgga
    13861 gcgggtgctc aaatccgttt ggcgcgaata ccaccaaggc gacccggact ggatcgagcg
    13921 ctaccgccaa ctggtcggca cctccaccca ggtgcgctac acggccatct ccatcctgcg
    13981 caatctgctg gaggaataat gcgcgcgttt gcttaccaaa aacccctgcg ggcggttaat
    14041 ttcatctgcc acgcgccgga agcccgggcg gtcagcttgg tgggcgattt caacggctgg
    14101 gaccccgccg cgctgccgat gaaacagatg ccggatcgtt cctggctgct gacggtggag
    14161 ctgaagcatg ggcaccatcg ctatgccttt ctggtggacg gcgtcctgac tctcgacccc
    14221 cacgcccaag gcatcacccg caatgaaaag ggcgaacggg tctgcctgat cccggtcagc
    14281 taacccgggc cgcgcccgca agaaccggtt gcattcgggc cccgatcggc ccatccttgc
    14341 gccatgcacc agatgcgttg cctggcggtt atgcttgtga gtgtgttgac catgacggcg
    14401 gcggttccga acccggcgcc cgcggaggag cgcggggcgc gcctcgaccc ggcgttggtg
    14461 caatacttcc gccttgaaac cgcccggctg tccgagcgct gcctggccga catccattcg
    14521 ctcgcggact gggaggcgcg gcgcggggaa taccggcggc agttgcagga aatgctgggg
    14581 ctgtggcccg cgccggaacg gacggaactc cggccggtca tcaccggaaa actggagcaa
    14641 ctggatttca cggtcgagaa gctgcacttt caggcattgc cgggcctgta cgtgacggcc
    14701 aacctgtacc gcccgtggaa ggtggaagac ccgctgccgg ccatccttta cctgtgcggc
    14761 cacagccggg tgatgaccaa cggcgtgagc tgcggcaaca aagccggcta ccagcaccac
    14821 ggcgcctggt ttgcgcgcaa cggctacgtc tgcctggtga ttgatacggt gcagttgggc
    14881 gaaatccccg gccagcatca cggcacctac agcgacgggc aatggtggtg gaacgcgcgc
    14941 ggctacaccc cggccggcgt cgaagcctgg ttcggcatcc gggcgctgga ttacctgtgc
    15001 tcgcgcccgg aggtggacaa ggaacgcatc ggcctgaccg gccggtcggg cggcggctcc
    15061 tacacctgga ccgtggcggc gctggacgag cgcgtcaagg tcgccgcccc ggtggcgggc
    15121 atcaccgacc tgcgcaacca ggtggtggat ggctgcgtcg agggccactg cgattgcatg
    15181 ttctacgtca acacctaccg ctgggacttc ccccaggccg ccgccctgct ggcgccccgg
    15241 ccgctgctga tcggcaacac cgacaaagac cagatattcc cgctggacgg cgtggtgcgg
    15301 ctctataacc aggtgcggcg catctacgcc ctctacggca aaaccaacca cctcggactc
    15361 ctcatcaccg aaggcccgca cgcggacacc caggacctgc aggtgccggt cttccgctgg
    15421 ttcaaccggt ttctcaaagg ccagacgccg cccatcgaaa tggcggccgt gaagttcttt
    15481 gcccccgagc aactccgggt cttcgactcg ctgcccgccg acgagaaaaa caccaccatc
    15541 ccggaaagct tcgtgccccg ggcggccaaa tcacctccgc ccgccgaata tcggaaagcg
    15601 ctgcgcgaaa aggtgttcgg cggctggccg gcggagagcg ggacgccgcc gctggaactg
    15661 cgggagcggg cggaccgggc cggcctgcgc gtcggggtct atgtcctgga aagccagccg
    15721 gcggtgaagc tgccgctctg ggtactgcag gcccccaaaa cccggcgccc cgagcaggtg
    15781 ctgctgaccg tgctcgatgc ggagcgctgg acccattcac ccgccaaagg gttgtggcgg
    15841 ggcggcgaag ccccggaaac cgacgcccat ttgcgccagg aaatccaggc cggaaaactg
    15901 gccctggcgt tcttcgcccc gcgcgaagtc gaacccgcca gttggcagac cgaccccaaa
    15961 ctggcccgcc aggtccggcg ccgctacatg ctgctggggc aaacgctgga tggcatgcgg
    16021 gtgtgggaca tccggcgcgc cgtccaggcg ctcaaaacgc tgcccgagtt caagtccgcg
    16081 gcgctgggca tcaacgcgcg gcaacagatg ggcgtcaacg ccgcctacgc ggccctgttc
    16141 gagccggaaa tcacacaact ccagctcgaa ggactgccgg cgtctcatca ggaaggcccg
    16201 gactacctca atgtgctgaa agtgggagac atcccccagg tgctcgccct gctgggcccg
    16261 cgggtcgccg ccccggcgcg ctgagaaccc ggcgcgcccg tttgcgcgcg gcttcggccg
    16321 ccccgccgcg tcaagagctg gcggccgggt tgaataaacc atgcgcgccg cctccgagcc
    16381 gcggccagcc gccaaatggc gcccgggacg gtcatttctc tgaatctccg gccggccgac
    16441 tgtgctattc caccgcttgg cctgaatgca gaggtatcca ggccgggctt gcttctttga
    16501 tgatgccgtc aatcacccgg agaacaactc cggccccagc gcgcgcagcg tggcgcggat
    16561 ttgcgctttg ctccggccca gcgcgcggag caaatcaatg taattgtcca cgacggcttg
    16621 caaatgcgga tgctgatgcc gcgtggcgcg ggtgaaggcc accagtatct ccaagccccg
    16681 ccgcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctgc agcaatcgca
    16801 ggctcagcag ccgccgcacc acacggttcc acggatcggg atagcccggc tccgcatccc
    16861 gccccaactc cggaaacgtc ctggccgcca gccctcgcag ccacggcaac gccacctggt
    16921 cggacggcag caacgccgcc aggcgcaaca ccagccgttc ctcctccccc agccgctcca
    16981 gcgtcggcgc cagcgtcgcg cttaaacacc cctccccatg ccgcacccga acctccccct
    17041 gcctcaccgc ctgatccagc cccaccaacc cctcccgctc cagccgcgcc agaaacgccg
    17101 cgcaactgac ctccccccca tactgcccca gatacaccgc cgccgcttcc accgccaggg
    17161 tgaagttccc cagccgccgc actatctctg cggcggccgc ccgctccgtc tcgtggcgaa
    17221 atcgcccccc ccggctggta gctttccatg agcgccagcg cctcgtcccc cggcagttcg
    17281 tcgaggggca gaaacgcccg gtcgcggtgc gtgcccccca gttcgttctc ccccagccgc
    17341 gtggtggcca gcacgtgcag ccaatcgccc ccattgagcc gcgccaccat cgccggatcc
    17401 agcagcccgg gctgatccac gttgtccaaa atcagcaggc agcgcggcgg attcccgccc
    17461 gcggcaagtt gctgcaactc gcggcgaacc cgctcaaaca gccggtcggc gctcttcttt
    17521 tcgtcctcgg tgaactcaaa tcccactgcc ggagccagtt cggccagcgc caggcgcaaa
    17581 tcctcccggc ccgcgcagcg caccagccag cgccccccgc cgtattcctc ggcgtaggcg
    17641 tgcgcatatt cggtggccag cgcggtcttg cccaggcccc ccatgccgtg gacggccgtc
    17701 agcacgctaa tccggcccgg ctccacaaag ttctgccgca actgccgcaa cggccgcctg
    17761 cgacccacga agtgcggatt gtgcgcctcc acgttgccgg ggcttcgcgc cgcgcgttcg
    17821 ccgtgctgga tggcgtccca tatccggtgg ctgagttcgg ccatgcgttc ctgcacggcc
    17881 gcttcgagca gcgccgctgc gccctggtca taccacaggc gcaggtcgaa gtactggcgc
    17941 cgtcgcaact cgtccgccca cgcctcgttt tgctgcttat cacccttctg ctcccagctc
    18001 ggcacttcta ccaggtaaac aggcacaaca ccatgtgaac cacccgcgcg gccaatctcg
    18061 tgcttgaggt attcgttgaa ttcccacttg caattctgac tcgccaaata ccccggggaa
    18121 agacaggcca gcagcaaatg cgagtcgcgc agcccctgca aaatccgcag ccgccagtcc
    18181 tccatccccc gaatctcctc ctggtcgaaa aacacccgca gttcgcgctg cgcgtgcttc
    18241 tggaaatccg cctggatgtg ccgcaacagc tccgtgatgc ggccttgggc attgtcaccc
    18301 cgcgaatacg agaggaacag atcgtaaccc atacctgccc cagtctggcc acgggacgac
    18361 ggtttgaaaa gcccaatatc ccaggcccgc ccgggcccgc cagtatcttt ggttttgcag
    18421 atcaggaacg gaggaagttg aggctgcgga ttgggcggcg gttaatggcc ataaaagagt
    18481 tggagccacg gaaacagcca atagtgcccc ggcgggacac gggaaaggca gccgtgcgat
    18541 tcagggggcg ttgggccgct ctttttgact gtgcggtgat tgcgccgcac gatcagggtg
    18601 cgggcaaaag ggaagccggt ttgcagaggc tcaagggcgc aggggtgcag gacttccttt
    18661 cctcctcaat cccgcagttt caactacaaa agccacccta aaaaccgccc ccaaagtccc
    18721 tcggactccc aatctcatta aaaaacctca acctactgca atcacgctac tttcacgcta
    18781 ccctcaagcc gtgtacaagg ggtgtacaag gggtgctcat cggaggtacg acagaaaaat
    18841 tcatgtacct ccggcgagca tcccttgtac accccttgta cacggcttga gggtaggtat
    18901 ggatgaggct ggaaagacgg gggttacgtg aaaaacgggg gggtctccac ggattcgata
    18961 aaacagggca ggaggatggg cgggcttggg gtatggcgcc gggagccttg tttcacgagt
    19021 tgttgggatt gggattgaat tggggggtgg atgtgagctg atttgaagct gcaagcggca
    19081 tggcgtttct gaagttccgg gagcggctgg cattttgggg atcggcttgt ggcccccagg
    19141 atggcgggcg agtggtctgc gatggccaca ccttcagcgt atttgctgat tgggtggccc
    19201 tgcgggtgat ttttgccatt gaaggccatg acccggaggc gggggcggtt tttaccgggt
    19261 ttgggcaggg tgtccttcga ggggggccgg atttgaggct cggggaggcg gtttaatttt
    19321 tttaagcaat cggacttgac gaatggcgcg gggctcagta aattagccgc gatgttagtg
    19381 aatattcgac aggttgccgg ggtggtcgtc gtcgtcgtag gcgacgccgt aggtgcttgt
    19441 cgaaggtaat ccaggtttcg acaagaaccc acggctggca gacggtcgtg ggttttttta
    19501 tgcacctgcg gcagccagcc tcaaccacaa aaaaccaaat gagcaagaca acggaacaga
    19561 ctaagaaaaa gccgggcagc aagcgtgcgg aacgcggtcc cgccatgtcc ggcagtgaaa
    19621 tcctggtggc ctgcctcgaa cgcgagggcg tggacaccat cttcgcctat ccgggcggag
    19681 ccagcatgga aatccaccag gccctgaccc ggtgcaagac gattcgcacg gtgctgccgc
    19741 ggcatgagca gggcggggcc tttgccgccg gcggttacgg gcgcgtgacc gggcgggcgg
    19801 gcgtttgcat ggccaccagc gggccgggcg cgaccaacct ggtcacgggg atcgcggatg
    19861 cctacatgga ctccgtgccg ctcgttgcca ttaccggcca ggtggtgcag gcgatgatcg
    19921 gcaagggcgc cttccaggag acggatattt tcggcatgac gctgccggtg gtgaagcaca
    19981 gctacctgat caccgacatc aatgatattc cgcgcatcgt taaagaggcc ttttatctcg
    20041 cgcagagcgg ccggcccggg ccggtgctga ttgacctgcc caagaacgtg cagcaggcgc
    20101 gcacgcagcc ggttttcccg aaggaaacca gcctgcgcag ctaccgggcc gacccgcggg
    20161 ccagcgagct ggcgctcaac gaaatcatcg ggctgatcga aaaatccgag cgcccggttc
    20221 tctacgtcgg gggcggcatc atctccggca acgccagcaa ggagttgcgg cagtttgcgg
    20281 aagcgacgca gattccggtc accaccaccc tgatgggctg cggcgctttt ccggaaacgc
    20341 atcccctgtc gctgcgctgg ctgggcatgc acggcgcggc gtttgcaaac tgggcggtga
    20401 acggcgaata ccatccggcc gcccgcccca cggagccggc gaagctgaaa gctgcgggcg
    20461 cggacctgct gctggcgttt ggcgtgcggt ttgacgatcg cgtgacgggc cgggtggata
    20521 agttctgcga gaagggcacg atcgttcacc tggatattga tccgtccgag attaacaaga
    20581 acaagccggt gagcctgccg attatcagcg atgtgaagtt cgccctgggc cgcctggcgg
    20641 cgatggtcaa ggcgcggccc ctgcagaaga agttcaccgc ctggcataag cagatcgcgc
    20701 ggtggaaagc ccgggcgccc ttcggctata gcgtgaccga agaggtcatg aaatcgcagc
    20761 acatgcgcga tcatctcaag ggccgggagc gcgaggtcat cctgccgcaa atggccatcg
    20821 agatgctgta cgagctgacc cgaggcgagg cgatcatcac caccggggtg ggccagcacc
    20881 agatgtgggc ggcgcagttc taccagttca aatacccgcg ccagttcgtg accagcgccg
    20941 ggctgggcgc gatgggcttt ggctacccgg ccgcgctggg cgccaaagtg gcgttcccca
    21001 accggcaggt tgtggacatt gacggcgatg gctccttcct gatgaacatt caggagctgg
    21061 ccacggcgca tatcgagaag attgccgcca aggtgatcgt gctgaacaat cagcacctgg
    21121 gaatggtgat gcagtgggaa gaccgcttct acgccggcaa ccgcggcaac acctacctgg
    21181 gcgacccgga acggcggaag cagatctatc ccgactacac cgccatgtgc cagagcttcg
    21241 gcgtgaaatg cgagcgggtc atgtatctaa aagacctccg gccggcgctc cagcgcatgt
    21301 tggacgccaa cgagccctac gtgctggacg tcatcgtgcc ctacaccgag catgtgctgc
    21361 cgtttatccc gtcggggcga acggtggccg atatgatctg gcaggcgtaa gccggcgccg
    21421 cggcgggatt tccggcgctt cccggggtgg ccgcgccgcc gccccggggg cttttttcgg
    21481 cccgcgcgtc gggcgttggc cgggggatgg atttggcgcg cgggcgcgcc cgcacccggc
    21541 gcccgggccg gttattgttt gacgacccgg accttcccgg tggcggcgtc gtagtccagc
    21601 ttcatgccgt agggcgcggt gggcagcttc ggaatgaatt tttcctcgac cagttcattg
    21661 aggtccctgg gattgcgccc atgttccacg ttgaacagtt ggatggcctg gttgagcacg
    21721 gcggtatcca ccaccttgtc cgccgttttg tgcgccttgt ccagcgcgcc caggtaatcc
    21781 gccggcgcgc tcaacgggct gctgcccggg gtggcggaat tggcgggcgg ggcgggcgcg
    21841 ccggcctttt cgccgcaacc agcgaggagt ccgctgacgg ccagcagaag gcagaatggt
    21901 gttttcatgc gcatagttta atctgcggcc gtggcaagcc aagcccagga atggcgggcg
    21961 cgcggcgggg cgtggcggcc gggcggcgcg ccgggttggg cggcttgatt ttcgcggcga
    22021 aagggcgctt tgaaaccggg acggcttgcg gtaggttatg ccaccgaatg aattagcgcg
    22081 catattatgg ctgttgaaat caacattacc taccacggcg ggctgcgctg cgcggccacg
    22141 catgaaccct cggggcagac gttgagcacc gatgcgccgc tggataacgg cggcaagggc
    22201 gaggcctttt cccccaccga cctggtggcc acggccctcg gcacctgcat ggcgacgatc
    22261 atggggcggg tggccgatca gaataacctg aacctggacg gcctccagat ccgcgtcctc
    22321 aaggagatgg ccacggaccc ggtgcgccgc atccgcgcgc tcaagacccg gctggtcttt
    22381 cctcgcgccg ccgcgttgtc cgccgccgat cgcgcgaagc tggaagccgc cgcgcgggcc
    22441 tgcccggtca agcagagcct gcacccggaa gtccagttgc cgattgaatt tgtgtatccg
    22501 gcgtgatgcc ggccgcgggg ccggcgccgg gcgaaggccg cccggccgcg ccctgggggc
    22561 ggctgtccgg gggccgcggt tcagccctcg cgggcctcag gcattccagc cgcggcggtt
    22621 gtcgcgcttc acaaactggg cggcggcggg aaggttgggc gaccgcatat tcgggccgtc
    22681 ccattccatg cggtagcctt cgccgacgcg caaggccacg caccccagca aaatgatttc
    22741 cgtgaggtag gccgcgatgt cgaagttcga gtaggcgggc acgccgtcct tcatcatgcg
    22801 gaaccattcc tccatgtggc ccggcgagcg ggggatggac tgcggcacgg ccttgcaggc
    22861 ttcgtgctgg tcgctcgcca catattcgga cgatcccttc atggccacga agaatcgcgc
    22921 gccgtaatcg tccggtgaaa acaccttgcc cttttcccca ataatcagcg cgccgctggc
    22981 cgggagcttg tcgtatgtcg caatgatttc atggatcgcg tcgccctgcg ggcgcagcgg
    23041 cttgagcggg tccgcgggtt tgccgtcata ccaccagaac ttgagcggcg gcaggtcgcc
    23101 ccgctgcggg aactcgaagc ggatgcggga ggtcttgggg aaagcctcgg gatacaggcg
    23161 ggaggccagt tcgcattcga ccacgttggg gtagccgagg ttgagcgccc ggaacggcat
    23221 gttgacggtg tggcaggcca tatccccgag cgccccggtg ccgaagtcgt accagccgcg
    23281 ccacgcgaac gggtgatacg cccctttctt gtacggccgc ttgcgcgccg ggcccagcca
    23341 caaatcccaa tccagctccg gcgggacggg gtcctgcccc gcgggccggt ccaggccctg
    23401 gggccagatg gggcgattcg accagacgtg aagctccagc ggtttgccaa tcacgcccgc
    23461 ctgcacgatt tccaccgccc ggcgcaggcc gctttcggcg ctgccctggt tgcccatctg
    23521 ggtggccagg ttcttgtctt tggccagttg ccgcacctgg cgcgcctcgc tgacggtctg
    23581 gacgaggggt ttctggcaga aacagtgttt gcccatcttc atcgcccgaa tcgccgcaac
    23641 gccgtgctgg tgatccgggg tggaaatcgt caccgcgtca atgtccttgc ccagttcgtc
    23701 cagcaacttg cggtagtccc ggtaccgttt gagctggggg aacttttcgg cgaccttttt
    23761 ggctttgcca tccaaagtct tctggtccac atcgcagagc gccacgatct ggccgccgca
    23821 atttatggcg tcgctgacgt cgctgtcgcc tttgccgccc acgccaatgc aggcgatatt
    23881 gatgcggctg ttggcgccga gcacccggct caccgagggg aaggccagcg tcatcgcgcc
    23941 ggcggccagg gcggtgcgcc ggagaaattg acggcgggtg aaggagtttg tttgcagggc
    24001 agttgctacc atgttgtttt gcatatttat aataatccgg gccgctacgc ggcagacgcg
    24061 acggccactt cgttgacaag aggtatccgc ccttttccgc ccggtgtcta ggataaaaat
    24121 gggggcggcc cggactttga aaattgcctc cgcgggttct gttgccgggg gctggattct
    24181 ttgccgccgt cgagcgggga ttgcgggact ccagcgagcc gccaaccctt gccggagcgc
    24241 ggctaattcg ggggaaaatg ggtgaagatt tttattgatt tcggttcgga aaaccggcat
    24301 tgtaactatt acatgtgccc aatactcgtt ttcggctgcg gatgccggtg gcaggggttt
    24361 ttcccgccgc gtcgccgggc aatcgggcaa cgaatctcag ttaatcgttg aaagaatcag
    24421 agtttgttat gtcatttatc ccacacacta tggccggttt tggtatggaa tctccgcaaa
    24481 cccgttgtct ccggacggcg attttcgccg cgctgttgct ggttctgggc acggcctgtc
    24541 cggccgcggc gccggagtat cgggcgttct gggtggacgc ctggaatgcc gggctgctga
    24601 gtcagagcca ggtggatacg ctgctcggcg tgccgggcaa tgccaacagc aaaggggtca
    24661 tccgcgaagc caactgcaat atggtgatcg tgcaggtgcg ccgccgtgcc gacgtttgct
    24721 atccttcggg cgtcggcgag ccctacatgt ccgggctgtc gccggccaac ttcaattccc
    24781 tggccgccat gattaaggcg gcgcacgaca ccaccggcgg caagaagcgc gtcgaggttc
    24841 attgctggtc ggtggttttc aagaccgcca aggggcaggt ttattcgcag catagcgatc
    24901 cgccgaccgg cagtctgacg acgtttgaca attactggcc cacgcgcctg agttcgacca
    24961 ccggcgcgga aaacagcgat ggggccttcg atcccggcca tcccaagtgc ctggagtatt
    25021 taatcaatgc ccacatggac ctggtgaact ttcagacgac cgccgggccc ggcggcgcgg
    25081 atgggcggat tgacggcatt cattacgact atatccggtt cgaggcggcc accgaggggt
    25141 tcaaccccac cagcgtcgcg cgctataacg cccgttacgg gttgagcggc aacccgtccg
    25201 ccgccagcga gcagttcaag cagtggcggc gcgatcaggt caccgccttc gcgcggcaga
    25261 tgtatgcgcg cattcagaag accagccccc agatccggca gtcgggttcg tttgtcacct
    25321 ggaatccctc gccggcgtcg tccacccgcg ccgcgtttca ggccacccgg ccgtactatg
    25381 acgtgtattc ggactgggat agctggcaac aggaagggat catggatatg gcggtgccga
    25441 tgacctacta caactgggcc agcctgccgt ctgactatac gaagtggatg aacttcgaga
    25501 aggaccgcaa gttcaaccgc cacatgatta tcgggccggg catttacttg aactcgctgg
    25561 ccaatgccat ttacgagctt caaatgaccc gggatgcctc gccggcgggg aattacgccc
    25621 acggcttcag cggctattcc taccggacgc cgtatagcgg cggcacctgg gccggctttg
    25681 ccccatcgtt ggtgtcgtcg gtcacgccga cttgggctga cattccggag atgccctgga
    25741 agacctcgcc caccaaggct catataatgg ggactgtgac gattcatggt tcgggggcct
    25801 gggccgacgg cgcccgggtg agcctgtcgg gcccggtcag ccgggtgcag acgaacgatg
    25861 ggacgggatt ttatgccttt atagatttgc cggtggggac ctataccgtc accgccagtc
    25921 tggcggggca tccgacggca accgggagca cgaccatcgc ggtggggcag gttaccggca
    25981 atatgtatga gctggacctg gttctcggcg gaaacgtccc gccgacgatc accacgcagc
    26041 cgatcagcca aagcgtcaag cagggaacca acgccacatt cacggtcggc gtcagcggca
    26101 ccgcgccgtt tgcgtatcaa tggcggctga atggggcgga catttcgggc gcgaccgcca
    26161 gcagctacac gcgcgccaat gtgcaacccg cggatgccgg gacgtattcg gtggtggtga
    26221 ccaatgctta cggaatggcc accagttcgg gcgcgctgct gacggtagtg gtgccgccga
    26281 ccatcaccac gcagccgctg agccagaccg tcacccaggg gcagaacgcc acgttcacgg
    26341 tggccgcctc aggcacggct ccgttcacgt atcaatggcg gtttaatggc gcgccgattg
    26401 gcggcgcgac cgccagcagc tacacgcgca gcaatgttca actgtcggat gccggcgatt
    26461 attcggtggt cgtggccaat tctgccggca gcgacaccag cgccaaagcg gtcctgaccg
    26521 tggtcgttcc gccggttccg cccgccatca cgacccagcc gcaaggccag acggtggtgt
    26581 tgggagagac ggccgcgttt aatgtcgtgg ctacgggtgg cattccgctg tcttaccagt
    26641 ggcgcttcaa cggggcggat attgccgggg ccacggacag cagctatgcc cgggcccagg
    26701 ttggcgcggc ggatgccggg gattattccg tgcgggtgac caacccctac gggagcctgc
    26761 tcagcgacag cgccacactg gtggtggata cgaacgtgac cctgccggtt atcgtgacgc
    26821 agccgcagag ccagacggtg atcgcggggc aaagcgcgac ctttagcgtg ctggcgacca
    26881 acaaggctcc gctgagctac cagtggcggt tgaatggggc gccgattgcc ggggcaacgg
    26941 gcagttccta tacccggagc aacgtccaga ctggtgatgc gggcaattat tcggtggtga
    27001 tcaccaatat tattggcgcg g
//
LOCUS       DAOPQG010000045        26948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2295_length_26948_cov_16.362883, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000045 DAOPQG010000000
VERSION     DAOPQG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 26948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26948
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2295_length_26948_cov_16.362883"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PKI07_06900"
     CDS             <1..1352
                     /locus_tag="PKI07_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC50820.1"
                     /translation="IAAWNMSAVVFFLPGRPPLWMALAAISFSIGVLQYTINRNMRFL
                     HVPSVTWPLLFLSLVVLITMRLTGGFGLRALGSDTYGGRNYIMFFATIIGYFALISRP
                     IPPKRALLYVALFFLGTSTMAIGDLPMLLPSGFNFVYLVFPLLGAGWAALQGATFEGP
                     VRITGFAFFNQGIFTAMLALYGIRGIFAGTAKSWRAVLFAACVFGALMGGFRSIVILF
                     FFTFAILFYMERLHHTRLLPTFIFIGLLGGALMITFAHRMPHAVQRSLAFLPVKLDPE
                     ARLSAAVSAEWRLKMWQELLPQIPQYLILGQGYAFSGQELNLLHDTRSGESSKLVGNY
                     HNGPLSVLIPFGSFGMLGFLWFLWAGLRVLYLNYQFGDPAYRTANNFLFAYFLVKAVF
                     FFVIFGAFISDMLMFVGMLGLSISLNGGVAKPALAPQPQVVVDRFKLRPGSRRPVSA"
     gene            1474..2988
                     /locus_tag="PKI07_06905"
     CDS             1474..2988
                     /locus_tag="PKI07_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOC50821.1"
                     /translation="MNTTPIHLAQSEAEVFLFPQMASRHGLIAGATGTGKTVTLQVLA
                     ESFSARGVPVFLADVKGDLAGISQPGGQSPKVKERVAQLRLDSYTPAGCPVIFWDVFG
                     EQGHPVRTTISEFGPLLLARLLQLNDTQAGVLNLIFKIADESGLLLLDLKDLRALAQY
                     AGENARQFTTQYGNISAASIGAIQRNLLVLEEQGADKYFGEPALNLDDLLQTDSNGRG
                     IVNLLAADKLLQSPRLYATLLLWMLSELFERLPEAGDLPQPKLVFFFDEAHLLFNEAP
                     PALLEKIEQVVRLIRSKGVGVYFVTQNPRDVPDNVLGQLGNRVQHALRAFTPRDQKAV
                     IAAAETFRPNPKFRTADAIKELAVGEALVSCLEENGQPAIVQRAWIVPPRSQIGPITP
                     EQRRQLMQSSIVAGAYDTAIDRESAYERLAAVQSPAAAPAPPPGKSGSPGPIFSGGTP
                     AAPRSRPAGRQPDSFVTMAAKSAVRSVSSSIGRQIANQVMRGVLGSIFGGGRRR"
     gene            3115..3867
                     /locus_tag="PKI07_06910"
                     /pseudo
     CDS             3115..3867
                     /locus_tag="PKI07_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4203..4604)
                     /locus_tag="PKI07_06915"
     CDS             complement(4203..4604)
                     /locus_tag="PKI07_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50822.1"
                     /translation="MRHFEAFYDEDGSCLPGKSTVVALPMEEKASGKFLLGLLNSVIP
                     KVFFRECYGALAMDGGITFTNKNCGEIPIPKRIGNAQQRVERLVDRILAAKQRDPTAD
                     TSALEREIDGLVYALYGLTPEEIKIVEGAAK"
     gene            5269..6123
                     /gene="rplB"
                     /locus_tag="PKI07_06920"
     CDS             5269..6123
                     /gene="rplB"
                     /locus_tag="PKI07_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOC50823.1"
                     /translation="MPVKTFRPLTPSLRYIAYSDYSDITKTKPERRLVETRKRTGGRN
                     VYGRITARGIGGGHKQKIRLVDFKRNKYGVEATVKAIEYDPRRTARLALLEYADGEKT
                     YIIAPSGLQVGAKLMSGPKAPPEVGNSLPLRTVPVGLPIHNIEIVPGRGAQMVRTAGG
                     AATLMSRDEGYAQIRLPSGEIRLVNENCYATIGQVGNAEHENVVLGKAGRSRHLGIRP
                     INRGVTRNPVDHPNGGGAGKSKSGGGWQQLTSPWGLLAKGYRTRNRKKHSNRFILVRR
                     DGRPMKNK"
     gene            6130..6411
                     /gene="rpsS"
                     /locus_tag="PKI07_06925"
     CDS             6130..6411
                     /gene="rpsS"
                     /locus_tag="PKI07_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOC50824.1"
                     /translation="MGRSIKKGPFIDEHLLAKVSLAKETKSKAPIKTWSRRSMITPDF
                     VGLTFNVHNGKIFNAVFVTENMVGHRLGEFSPTRTFRKHGAHTAKAEAK"
     gene            6416..6769
                     /locus_tag="PKI07_06930"
     CDS             6416..6769
                     /locus_tag="PKI07_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOC50825.1"
                     /translation="MEVQAITRNVRMSAQKMREVVRQIQGLPAVRAQAVLAVVPRKGA
                     RLVAKTLRSAIANAADLKEHKKEYSGLRVDRLVIKEALAQTAASYKRFTPKARGSAGP
                     IIKRNCHIKIVLSDE"
     gene            6772..7440
                     /gene="rpsC"
                     /locus_tag="PKI07_06935"
     CDS             6772..7440
                     /gene="rpsC"
                     /locus_tag="PKI07_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOC50826.1"
                     /translation="MGQKTNPVGLRMAVNHEWRSRWYAGKKDFGALLSEDRKIRALLK
                     KKLESASVPQIRIERAASRCRITIMTARPGIVIGRKGAEIDKIKEDLSLMTGKEIYVD
                     IEEVKSPEIDAQLVAENVALQLERRVSFRRAMKKALQTAKDFGAEGIKIRCAGRLGGA
                     ELARVEQYHWGRVPLHTLRASIDYGFAEANTLYGKLGIKCWICKGDVKAEKAPATASA
                     AGAN"
     gene            7462..7881
                     /gene="rplP"
                     /locus_tag="PKI07_06940"
     CDS             7462..7881
                     /gene="rplP"
                     /locus_tag="PKI07_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOC50827.1"
                     /translation="MPLMPERVKYRKMHRGNRAGLATRGHTVAFGEYGLMALERCWLD
                     TKQIEAVRVAVARNMKRHGKMWIRVFPQKSYTKKPLETRMGKGKGPVESWVAVVRPAN
                     VILEVDGVSETVARESLRLAATKLPIRTKFISRHRRV"
     gene            7921..8127
                     /gene="rpmC"
                     /locus_tag="PKI07_06945"
     CDS             7921..8127
                     /gene="rpmC"
                     /locus_tag="PKI07_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOC50828.1"
                     /translation="MKKAQRKEIQEKTLAELLAEARNWRQEIFNLRLQQASAQLEKPA
                     RLRTLRRDIARIETRISQLRRKAA"
     gene            8137..8442
                     /gene="rpsQ"
                     /locus_tag="PKI07_06950"
     CDS             8137..8442
                     /gene="rpsQ"
                     /locus_tag="PKI07_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007320505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOC50829.1"
                     /translation="MAETVITAEPGRAQRKQRVGVVIANKMAKTITVEIMRRYPHPQY
                     KKVVTGYKKLYAHDEKGEAKVGDRVRVEETRPLSRTKRWRLVEVVERGSGAAPVAAG"
     gene            8478..8843
                     /gene="rplN"
                     /locus_tag="PKI07_06955"
     CDS             8478..8843
                     /gene="rplN"
                     /locus_tag="PKI07_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOC50830.1"
                     /translation="MLQVRSILDVADNTGARRAAAIGVLGRNQQYARVGDVIKAHIKE
                     AAPDGTVKKGEVVDAVVVRTRQVIRRNDGSYLRFDSNAIVLIDKEFNPRGTRIFGPVA
                     RELRDKKFMKIISLAPEVI"
     gene            8862..9140
                     /locus_tag="PKI07_06960"
     CDS             8862..9140
                     /locus_tag="PKI07_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOC50831.1"
                     /translation="MRKCHIKKGDEAVVIAGVDKGRRGRVIAVIAKKQRVIIEGARMI
                     KKHMRKSQQYPQGQIVEREGSIHISNVMKAEKYDARAARRGAAAPART"
     gene            9167..9778
                     /gene="rplE"
                     /locus_tag="PKI07_06965"
     CDS             9167..9778
                     /gene="rplE"
                     /locus_tag="PKI07_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOC50832.1"
                     /translation="MKARLYERYIKEVVPALREKHKHANPHQVPRMEKIVINMGVSAS
                     LEKSAVDDAAKDLALITGRKPAISKSRHNIANFKLRRGMPIGCRVTLRRDAMYEFFDR
                     LIATALPRIRDFRGLSPRSFDGRGNYSLGVGDQTIFPEIELDKIKRQQGMDITIVTSA
                     RTNEEAFDLLKLMGMPFAEARQAEVRRAPAPASEAQQDKTEQT"
     gene            9781..10050
                     /gene="rpsN"
                     /locus_tag="PKI07_06970"
     CDS             9781..10050
                     /gene="rpsN"
                     /locus_tag="PKI07_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOC50833.1"
                     /translation="MAKTAWIERNKKKAETVKKYAAIRAELKARRDYAGLAKLPRNAS
                     PSRVVNRCSMSGRRHGYLRKFGCSRLTFRESALNGLIPGVTKASW"
     gene            10055..10441
                     /gene="rpsH"
                     /locus_tag="PKI07_06975"
     CDS             10055..10441
                     /gene="rpsH"
                     /locus_tag="PKI07_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOC50834.1"
                     /translation="MNDPISDMLTRIRNASRALLPAVEVPHSRIKEGIAGILKREGYI
                     ADFAVERKTPRTLTLKLKYQGKKSIIEGLRRVSTPGLRRYVGAAEIPRVRGGLGVAVL
                     STSEGLLTDVQARRKRIGGELICYVW"
     gene            10542..11078
                     /gene="rplF"
                     /locus_tag="PKI07_06980"
     CDS             10542..11078
                     /gene="rplF"
                     /locus_tag="PKI07_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOC50835.1"
                     /translation="MSRIGKLPIAIPPKVKVDIKGQQVFVEGPKGKLNWELPRRTGLK
                     VENGKVVVSRESDDARVKALHGLSRALVNNMVRGVSEGFVKKLEIQGVGFKAAVADNK
                     VTLNLGYSHPIVYAIPAQIKVTVEENTRLTIEGPDRQLVGRVAAELRSFYPPEPYKGK
                     GVRYSDEHVIRKEGKTVQ"
     gene            11152..11517
                     /gene="rplR"
                     /locus_tag="PKI07_06985"
     CDS             11152..11517
                     /gene="rplR"
                     /locus_tag="PKI07_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOC50836.1"
                     /translation="MRTEQKQRLAQRRRWRIRRKVSGTKECPRMSVCFTNKNIHVQFI
                     DDTAGVTLGAVSTVSKAVSRAEKLAANAVSARKIGELAAQTALDKGIKRVVFDRGRAR
                     YHGKVKALADAAREKGLQF"
     gene            11526..12185
                     /gene="rpsE"
                     /locus_tag="PKI07_06990"
     CDS             11526..12185
                     /gene="rpsE"
                     /locus_tag="PKI07_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOC50837.1"
                     /translation="METNIVAEPAKVEGAGPRAPRGPGRGRRSRPEPEPTFDEQSGQE
                     LTEKVVFINRSAKVVKGGRRFNFSALIVVGDRHGRVGVGLGKAGEVADAIRKGGEMAR
                     SQMMTVSLKEATIPHEVYSHYCGAKVLLRPASPGTGIIAGKTVRAVLESAGVKDVLTK
                     SLGSKNAANVVKATLNALLSLRLREDIYSGRGLEIKKTPLPQEPEPLSPLAPPADSLT
                     A"
     gene            12192..12680
                     /gene="rplO"
                     /locus_tag="PKI07_06995"
     CDS             12192..12680
                     /gene="rplO"
                     /locus_tag="PKI07_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOC50838.1"
                     /translation="MRLHNLQPRPGARHRRKRLGQGEASGHGKTSGRGGKGQTARSGS
                     SIRIGFEGGQMPLIRRIPKRGFNNARHTKRYLPVNLESLNQFADGTRVDLESLRSKGL
                     ANGSAAGIKILGSGELTRKLTVSAHAFSASARAKIEAKGGACELIAPKGAKRANPEAA
                     KR"
     gene            12769..14163
                     /gene="secY"
                     /locus_tag="PKI07_07000"
     CDS             12769..14163
                     /gene="secY"
                     /locus_tag="PKI07_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOC50839.1"
                     /translation="MLVAIYNTFRNCFKIPELKSRILFTLMVLAICRLEAVVRIPGLD
                     GTTLANYFASKAGSAEGASVLGMYSLFTGGALEHCAVGALGIMPYISATIIIQLLTAV
                     IPTLSKLAREEGGRTKIVQYGRYLTVILCLGQGMVMAIGWENPEKLFPGWNTADRLVL
                     YSDAHIWFYRIQTLLLLTTGTMLLMWLGEQITDRGIGNGVSLVITIGILARLPQAMVA
                     LKDMFFPGGGMEAKFNFGHAIALVLLLAGVIAGVIAITQAQRKVPVQYAQRAVGRKVY
                     SGGTSFMPLRVNYSGVMPIIFAQAILMFPQKIFLFLGQSLNVKFFGEIARNLGEGAIL
                     YMTLYGLMILFFSYFWVATQFNELQIADDLKKNGGYIPGVRPGQATSDYLHNAMSRIT
                     LAGAIFLTIIALIPIWLWRELQIPYSVATFFGGTSMLILVGVLLDTMRQTETHLLMRH
                     YDGFLKKGRLRGRF"
     gene            14163..14927
                     /gene="map"
                     /locus_tag="PKI07_07005"
     CDS             14163..14927
                     /gene="map"
                     /locus_tag="PKI07_07005"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOC50840.1"
                     /translation="MIILKSERDLEAMRPACVVAATVLNDVAAFIQPGVSTKQVDEFA
                     ASRIKQLGARSAFLGYRKYPCHTCISVNDQVVHGLAGNRVLQFGDIVSLDVGVIHNGF
                     IGDTARTLAVGGCGVLAQRLMDVTEKGLYLGIAQARAGGKVSDISRAIQDYVEGNGFN
                     VVREFVGHGVGRSMHEDPQIPNFTDGKPSPKLRAGMTLAIEPMVNAGGPEVKMLNDGW
                     TAVTKDGSLSAHFEHTVLVTESEPEILTCLEKVSLK"
     gene            15127..15240
                     /gene="rpmJ"
                     /locus_tag="PKI07_07010"
     CDS             15127..15240
                     /gene="rpmJ"
                     /locus_tag="PKI07_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOC50841.1"
                     /translation="MKVRASVKKLCENCKIIRRRGVIRVICSNPRHKQRQG"
     gene            15280..15675
                     /gene="rpsM"
                     /locus_tag="PKI07_07015"
     CDS             15280..15675
                     /gene="rpsM"
                     /locus_tag="PKI07_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOC50842.1"
                     /translation="MARIIGVEVPPNKRIDIALRYLYGLGPSNSRAILAKAGIDPSIR
                     ARDLTEQQLSQIVHAIQDGKYVIEGDLRREIGMNLKRLQGIKCYRGIRHLRGLPVRGQ
                     RTQTNARTRKGPRKTVGVVRSPTAKSGIH"
     gene            15724..16386
                     /gene="rpsK"
                     /locus_tag="PKI07_07020"
     CDS             15724..16386
                     /gene="rpsK"
                     /locus_tag="PKI07_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOC50843.1"
                     /translation="MADETKKKSTPLSEGKPGGKKPMTEEKAVAAKAGEKSTAQAEAA
                     APAAKARKKADRTTENTAAPAEAPKAAEGGTPVAAGAAPTAAELLADDLAGKKIIKAK
                     GAKNIVSGIANILATFNNTLVSITDMQGHVIGWSSGGRVGFKGSRKSTAYAAQQVAQD
                     AARQAMSHGMREVEVRVNGPGSGRESAIRAFQAIGLEITAIKDVTPVPHNGCRPRKKR
                     RV"
     gene            16422..17030
                     /gene="rpsD"
                     /locus_tag="PKI07_07025"
     CDS             16422..17030
                     /gene="rpsD"
                     /locus_tag="PKI07_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOC50844.1"
                     /translation="MARYTGPRVRISRRFGVPIFGPSKYLERRNYGPGVHGPKSRRKH
                     TDYALGLIEKQKLRYYYGLLERQFHGVYERALQRRGITGEHMLQILETRLDNVVYHLG
                     FANTRATARQLVAHGHVRVNGRKVSIPSFGLKVNDSVEVKNSTVSRQLASRNLESAAS
                     RAVPDWLSLDKEAFKGVVMRIPTREEIQPIANEQAVVEFYSR"
     gene            17198..18223
                     /locus_tag="PKI07_07030"
     CDS             17198..18223
                     /locus_tag="PKI07_07030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOC50845.1"
                     /translation="MPVRLGRFEMPKRLVKEDLTATATYARFVAEPFETGYGYTIGNS
                     LRRVLLSSLEGAAISSIKITGAMHEFTIVDGVVEDVTEIVLNLKKILFKAHTRDPQTL
                     FLSVNREGAVTAADIQLNQNLELVNPDQHICTLDKKKKLEMELEVKIGRGFLPGEENK
                     RTNQAIGVIAIDSLFSPVTRVRYAVENARVGQRTDYDRLILEIWTDGRISPEDALTQA
                     SAILQHHLDIFVGYDKNAVEFEEIVDKQDEEKTRLKKLLNMSVNEIELSVRAANCLNN
                     ANITTVGQLAMKTEQEMLKYRNFGKKSLNEIKEKLAGLNLTLGMTFEAELLEATKEKP
                     LVLPTGR"
     gene            18297..18749
                     /gene="rplQ"
                     /locus_tag="PKI07_07035"
     CDS             18297..18749
                     /gene="rplQ"
                     /locus_tag="PKI07_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOC50846.1"
                     /translation="MRHLKRTAKLGRTGQHRNAMLANLVCSLIKHKRVITTLAKAKAA
                     RSVAEKMVTLGKCGTLHDRRLAAARLHQEDAVKILFNEIAPAQKERRGGYTRIIRLGQ
                     RQGDAAQRAILEWVELGTVAGTPAKESASTKKAKPVETKVDSKAESEK"
     gene            complement(18835..20166)
                     /gene="amrS"
                     /locus_tag="PKI07_07040"
     CDS             complement(18835..20166)
                     /gene="amrS"
                     /locus_tag="PKI07_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOC50847.1"
                     /translation="MNGKFKAQRRAFGKRAKAEGQKSGQRVFSRRAAIGLGVAGAASL
                     AGGASWFDFFTRWREAEMPADIFKGDAPKGELWELWRRRGWVKEASHHLKLGRNVQCK
                     VCPNNCLLAPGDRSHCRNKVNCDGILYTLAYANPCTFHVDPVEKKPLFHFLPGSRTFS
                     LATSGCVFRCLNCQNWEISQKKPEETKDAHGPELRVQPPLPAGFTLSQMARLSLFPED
                     APAVAKALDCPSIAYTYSEPTAFYEYTLDTCKAAREHKLKNIVVSCGSIEERAARALY
                     QYVDAAHVDLKGFDQNIYTKLNSGKLQPILNTLKTLRDLGIWFEIINLVVPTYTDEID
                     TIRRMCAWISKELGPDQPLHFSRFHPQHKLTYLTPTPVETLVTARNVARSEGLRYVYI
                     GNVPGLEDAETTWCPNCKQAVIERDIYAVTSFRLTNGKCPSCGNKIAGVWT"
     gene            21085..22371
                     /locus_tag="PKI07_07045"
     CDS             21085..22371
                     /locus_tag="PKI07_07045"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOC50848.1"
                     /translation="MDKTAMLTLDGKSYTLPIIAGSEGERAIDVSTLRNQTGCITLDE
                     GYGNTGSCRSQITFIDGEKGILRYRGIPIEELAEKSTFVEVAHLLIMGRLPNSPELRY
                     FSDLLTDNELLDEAMKHHFEGFPAHAHPMAILSAMINAASCFHPELLTDRQHDCFLVQ
                     AARLLSKVRTIAAFAYRKSRGLPAIYPKPELKYTANFLHMMFSLPNRDYALTPEVVRA
                     LDLLFLLHADHEQNCSTATVRMAASSHANLFASAAAGVCSLWGPLHGGANQAVLEMLQ
                     AIHQAGDDGSRFIAAAKDKHSGKRLMGFGHRVYKHYDPRAKIIKRACDEVLAELHISD
                     PLLDIAKHLEEAALRDPYFIERKLYPNVDFYSGIIMRAIGIPVEMFTVVFAIGRMPGW
                     IANYKEVMESSARICRPRQIYTGPTLDHYTPLKERK"
     gene            22430..23638
                     /gene="sucC"
                     /locus_tag="PKI07_07050"
     CDS             22430..23638
                     /gene="sucC"
                     /locus_tag="PKI07_07050"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOC50849.1"
                     /translation="MNLHEYQARQLLDQAGVAQAAWEFCATPEAARDIAARFLGKGAT
                     LIAVKAQIHAGGRGRGTFKNGFQGGVRLCRTAEDVYEKARAMLGNVLVTRQTGPEGRL
                     VDKLIIAVAPDIRKEFYLAVLLDRVASRPVVMASTEGGMDIEEVAAQTPDKIIREHID
                     PAIGMMPYQARRVAVALGLRGNLIAQAVRLLLGVYRTWWECDATLVELNPLCIVKTPD
                     GSEMLQAVDAKLSLDDNALFRHPELLAMRDLAEEAPLEVEASRFNLSYIKLDGNIACL
                     VNGAGLAMATMDVIRHYGGHPANFLDIGGGASREQVAAALEIVLQDASVKGILVNIFG
                     GIMDCDIIANGIVAAARQTARKLPVVIRLEGNNVEAARKTLAESGQAFIYSNSMDDAA
                     QKIVKAVNSP"
     gene            23650..24540
                     /gene="sucD"
                     /locus_tag="PKI07_07055"
     CDS             23650..24540
                     /gene="sucD"
                     /locus_tag="PKI07_07055"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOC50850.1"
                     /translation="MSILVKPDTKVIIQGIMGRFGSRHAQLSLAYGTQIVAGVSPGRG
                     GQRFANQVPIFDTVTEAVQQTGATTSAIFVPPPLAADAILEGVSAGLDLVVCITEGIP
                     VKDMIKVKRAMTGKPTLLIGPNCPGIVTPGGSQDSPRGCRIGIGPGYIYRPGSIGVVS
                     RSGTLGYEAVWQLTCRGLGQSTFVGIGGDPVHGISHLDAIKRFAADPETQGIIMIGEI
                     GGTAEEEAAAWIRDNCRKPVVGFVAGSTAPPGRRMGHAGAIISGGQGTAAAKISAFRE
                     AGIGVAMTPAEIVDTLVRML"
     gene            24624..25481
                     /locus_tag="PKI07_07060"
     CDS             24624..25481
                     /locus_tag="PKI07_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOC50851.1"
                     /translation="MGLIDLVLNLAGTLLWLGWRSMGLDPLVKATPVSLVGTLRRAEP
                     RRLRGWQLLVGLTALLLLRAALYCQIGPEVNWTPKLNLFFVVLAFHSHLFLPSLLYSI
                     LSFGRLLIICYFWMLVLVIINRRTGSPDPVLKALRAHLSAVARWPRLLQWGLPLLSVA
                     ALWIALHPLLVQQGIDQPAQTHARLAGQGMLVGAALYLSLQYLLPGFLFAHLVASYVY
                     LGDSPLWDFINATARNLLAPLRRLPLQIAHVDLAPVVGVALIFVLLHWLPDLIVRKMA
                     LHQISFWPQ"
     gene            complement(25506..26606)
                     /locus_tag="PKI07_07065"
     CDS             complement(25506..26606)
                     /locus_tag="PKI07_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOC50852.1"
                     /translation="MKRILVIDDEEWLREMVRQALQQKGYEVIEAEDGVAGVEKARRE
                     LPDLVLCDVNMEKMDGYLTLSALRQEPSAAAVPFILMTGSADNAGMRQGMELGADDYL
                     PKPFTLDALYAAVETRLKKAQVVRQEAEKKLTDLRNSISLMLPHELRTPLNGILAYGE
                     ILATGAESLPASEIAEMGQTIHDSGKQLERLIENFLIFAQIELLGTDAQKIGALRLNQ
                     TDSPARLIEERARKQARAANRLEDLGLNLADLPMPISEEYLAKIVDELVQNAFKFSER
                     GSAVRVALSDGPNGVALSVADRGRGFSTEHITRIGAYMQFDRKMQEQQGLGLGLVIAK
                     RLAELHGGTLTIQSERGTATTVTLKLPKVVAT"
BASE COUNT         5598 a         7323 c         8315 g         5712 t
ORIGIN      
        1 tcattgccgc ctggaacatg tcagcggtgg tgttctttct gcccggtcgg ccgccgctct
       61 ggatggcgct ggccgccatc agcttttcta tcggcgtgct gcagtacact atcaaccgca
      121 atatgcggtt tttgcacgtg ccgtcggtga cgtggccgtt gctgtttcta tcgctggtcg
      181 ttttgatcac aatgcgccta acaggcggct ttgggcttcg ggccctgggc agcgatactt
      241 acgggggcag gaactacatc atgttctttg cgacgattat cgggtatttc gccctcatta
      301 gccgcccgat cccgcccaaa cgggccctgc tctatgtcgc gctctttttc ctgggaacct
      361 cgaccatggc cattggcgat ttgcccatgt tgctcccttc gggcttcaac tttgtatact
      421 tggtgtttcc gctgttgggt gcgggttggg cggcgttgca gggagccacc ttcgaggggc
      481 ccgtccgcat cacgggcttt gcttttttca accaggggat ttttaccgcc atgctggctt
      541 tgtatggcat ccggggaatc tttgcgggaa cggcgaaatc ctggcgtgct gtcctttttg
      601 cggcttgcgt tttcggcgct ttaatgggcg gctttcgatc catcgtgatc ctcttcttct
      661 ttacttttgc aatattgttc tatatggagc gattgcatca tacgcgattg ttgcccacct
      721 ttattttcat cgggctgctt ggtggggcct tgatgatcac gtttgctcat cgaatgccac
      781 acgcggtgca acgttccctg gcttttttac cagttaagct tgatcccgag gcccggttga
      841 gtgcggccgt ttctgccgaa tggcgcttga agatgtggca ggaacttctc ccgcaaatcc
      901 cgcagtatct gatcctcggg cagggctatg ccttcagcgg ccaggaattg aacttgttgc
      961 atgacacccg cagcggagaa agcagcaagt tggtaggaaa ctatcataat ggcccgcttt
     1021 cggttcttat tcccttcggc tctttcggca tgcttggttt cctttggttc ctgtgggccg
     1081 gcctgcgcgt tctttaccta aactaccagt tcggcgatcc ggcttaccgc accgccaaca
     1141 actttttgtt tgcctacttc ctggtgaaag ccgtcttctt ctttgtaatc tttggcgcct
     1201 tcatttcaga catgctaatg ttcgtcggaa tgctgggatt aagcatcagc cttaacggcg
     1261 gagtcgccaa gccggcactc gctccgcagc cccaggtcgt cgtagatcgt ttcaaactgc
     1321 gaccgggctc ccgccgaccg gtgagcgcct aagaaggagc gggaatgatc cgcaaggggc
     1381 ctggcgcggc ggaaatttcc gcaaaaccaa aagcccccgc gccgaagttg actcgcaaaa
     1441 cccactcgca ccataattcc gcccggtcat attatgaaca ccacgcctat tcatcttgcc
     1501 cagagcgaag ctgaggtctt cctttttccg cagatggcca gccgccacgg cctgattgcc
     1561 ggggcgaccg gcaccggcaa aaccgtaacc ctgcaagtcc tggcggaaag cttcagcgcc
     1621 cgcggcgtgc ctgtgttcct ggctgatgtg aagggcgacc tcgccggaat ttcgcaaccg
     1681 gggggccagt cccccaaggt caaggagcgc gtggctcagc tccggctcga ctcctacacg
     1741 cccgccggct gtcctgttat cttctgggac gtgtttggcg agcagggcca tcccgtacgc
     1801 accaccatct ccgaattcgg ccccctgctg ctggcgcgcc tgctgcagct taatgacacc
     1861 caggccggcg tgctcaatct gatcttcaag atcgccgacg agagcggcct cctgctcctg
     1921 gatctcaaag acctccgcgc cctggcgcaa tacgcggggg aaaacgcccg gcaattcacg
     1981 acgcagtatg gcaacatctc cgccgccagc atcggcgcca tccagcgcaa tctcctcgtg
     2041 ctggaagagc agggtgcgga caagtatttc ggagaacccg ccctcaacct cgacgatctg
     2101 ctccagaccg actccaacgg cagggggatc gtgaacctcc ttgccgcgga taagctgctg
     2161 caatcccccc gcctctacgc gactttgctg ctctggatgc tctcggaact gttcgagcgg
     2221 ctgccggaag ccggcgatct gccccaaccc aagctggtgt tcttctttga cgaagctcac
     2281 ctgctcttca acgaagcccc gcccgcgctg ctggagaaaa tcgaacaggt cgtgcgcctc
     2341 attcgttcca agggcgttgg cgtctatttc gtcactcaaa acccgcgcga cgtgcccgac
     2401 aatgtattgg gccaactcgg caaccgcgtg cagcacgcgc tgcgcgcctt caccccgcgc
     2461 gaccaaaaag cagtgatcgc cgcggcggaa accttccggc caaacccgaa gttcaggact
     2521 gccgatgcaa tcaaggaact ggcggtgggg gaagcgctcg tttcctgcct ggaagagaac
     2581 ggccagccgg caattgtcca acgcgcctgg attgtcccgc cgcgcagcca aatcggcccc
     2641 atcacgccgg agcaacgccg gcaactcatg cagagttcca tcgtcgcggg cgcctatgat
     2701 accgccatcg atcgcgagtc cgcctacgaa cggctcgcgg ccgtgcaatc gccggctgcc
     2761 gcacccgcgc cgccgccagg caaatccgga agccccggcc cgattttcag cggcggaaca
     2821 cccgccgctc cgcgcagccg ccccgcgggc cgccagccgg actcgttcgt caccatggcc
     2881 gccaaaagcg ccgttcgctc cgtcagcagc agcatcggcc gccagatcgc caaccaggtc
     2941 atgcgcggcg tcctggggag catttttggc ggcggccggc ggcgctagtg tagtgttcat
     3001 taaatcgatg ggcagaggtg ggtcttggca atactctggg ggggcatggc aagacctgtc
     3061 cgcggtttgg agatggattt cgatcaaagg agagcgctcg aatccctggc ccgaatgcca
     3121 acgacggccc cgcggagcgc gcagcgcgct cgcattcttc tggcctgcgc gggcgggttt
     3181 tcgcaggagg aagtggcccg ccaagtaggc gtgcgccgcc gcattgtgac caaatggtgc
     3241 gggcggtttc gcaagctggg gttggccggc ttggctgatg ctggcgggcg cggatgcaag
     3301 ccttcggtgt ctgaagagac ccgggccctg atcctgactc aagccacccg gccgcccaag
     3361 ggccggactc ggcacagcgt gcgttcgatg gctcgtaggg ccggcgtgtc ggtcggcacc
     3421 gttcacgccc tgtggcaggc caacgacatc aagccccacc tgacccgcac cttcaaatta
     3481 tccaacgacc cgcagtttga aacgaagttc tgggacgtga tcggtctcta tctgaatcca
     3541 cccgataaag cgctggtgct ttgttgcgat gagaagagcc agtgtcaggc gctggaacgc
     3601 acccagccgg gcctgccttt aggcctgggc cacattcgaa cgcgcacgca tgactacatc
     3661 cggcacggga cagttaccct ttttgccgca ctgtcttatc tggacggcaa gatcttgagc
     3721 cgcacggcgg agcaacacac ccacaaggaa tggctggctt ttcttaaggt gatcgaccgg
     3781 gaaactccct tggggcaggc gattcacctg attatggaca attatgccac acacaagcac
     3841 ccgaccgtta agtcctggct ggcccggggc cttttgtttt cggcgcctga gtttcggcgg
     3901 atgaggggga aacggtattt ccaaacccca ggccgctttg cgagttgagt gggcgggggt
     3961 gaaagcgtct atggattcaa aacggccggc cgacgtcgcg ggcaacgcgg cgccgcgcgc
     4021 gcgaacaggc cgaagggatg aaccccggaa gttggatacc ctgctgaatc aacggccccc
     4081 gttgggcgcg gggtggcgtg gttggggggc gttgcgccgg cgcggtcgga acggactcga
     4141 cccggccagc ggaattccgc gccgtgtcat ttcgtcctga atgtgggctg aaagttcgta
     4201 attcatttgg ccgcgccctc cacgatcttg atctcttccg gcgtaaggcc gtagagggca
     4261 tacaccaacc cgtctatctc ccgctccaac gcgctcgtgt ccgccgtggg atcgcgctgc
     4321 tttgcggcga ggatgcggtc aaccaggcgc tccacccgct gctgtgcatt ccctatcctc
     4381 ttaggtattg ggatttcgcc gcagttcttg tttgtgaagg taatcccccc gtccatcgcg
     4441 agtgcgccat agcattcgcg aaaaaatact ttcggaatga cggaattcag caagccgaga
     4501 aggaatttgc ctgacgcctt ttcttccatt ggtaacgcca caactgttga ctttcccggt
     4561 aaacagcttc cgtcctcgtc gtagaacgct tcaaaatgtc tcatccctga gagaataagc
     4621 ttgggacgtg tcgctcgatc gaacctgttc gggaaatgag tgtgcaaatc ttgcttggta
     4681 attatcggct ttgcgtactt cgacttgatg taagttgtgg gcttgatacc ccacaaacat
     4741 acatatggat cgcttgtccc ggtattcacg aatttaaaat agtccctttc tgttggtttc
     4801 cactctttca ggtgtttgat tatttcgtat gcctcggaaa tttcagcccg ctccagcctt
     4861 tttgtatcgg cctcggacgg ggcccgggca gggcggctgt ttccgcggcg gcgggatagc
     4921 ggctgcggtg ctgttttggc ggttcaataa ttgtgctggg cccgcgcttt tgtcttccgt
     4981 ggagcccggg gtaatgcctg ggccggcgtt gtcggggcgc gttttgatgg atgccgggag
     5041 gccggagcgg tggttctttc cgggggcttt ggagggtggc aattcggggg ggcaaaaaaa
     5101 atgtaaatac ctgttgcatt gcaaatggaa tctgctacag tgtgcgctcc gcttggcttt
     5161 gaagccgcgg gcagcataac ctgcgggacc gataactgac cgcccaaccg ggggcaggat
     5221 taatggttcc gctctccaag ttgctgactt gggaattgtt gtagatttat gccagtaaag
     5281 acatttagac cgctgacgcc gtcattgcgt tatatcgcat attcggatta cagcgacatt
     5341 acgaagacca agccggagcg ccgactggtt gagacgcgga agcgtaccgg cggccggaat
     5401 gtgtatggcc gtattaccgc gcgcgggatt ggcggcggcc acaagcagaa gatacggctg
     5461 gtggatttca agcgcaataa gtacggggtg gaagcgacgg taaaggcgat tgagtatgat
     5521 ccgcgccgga cggcgcggct ggccttgctg gagtatgcgg atggcgagaa gacgtatatc
     5581 atcgcgccga gcggtttgca ggtgggcgcc aagttgatga gcgggccgaa ggcgccgccc
     5641 gaggtgggca atagcctgcc gctgcgcacg gttccggtgg ggctgccgat tcataatatt
     5701 gagattgtgc cgggccgcgg cgcgcagatg gtgcgcacgg cgggaggggc ggcgacgctg
     5761 atgtcccgcg acgaggggta tgcgcagatt cggctgccgt ccggggagat tcggctggtg
     5821 aatgagaatt gctacgcgac gatcggccag gtgggcaacg ccgagcatga aaacgttgtg
     5881 ctgggcaagg cggggcgttc gcggcacctg ggcatccggc cgatcaaccg gggggttacc
     5941 cgcaatccgg tggatcatcc gaacgggggc ggggcgggca agtccaagtc cggcggcggc
     6001 tggcagcagc ttacctcgcc gtggggattg ctggccaagg gatatcgcac gcgcaaccgg
     6061 aagaagcatt cgaaccggtt tattttggtg cgccgcgatg gccggccaat gaagaacaag
     6121 taagtattta tgggacgttc catcaagaaa ggtccgttta tagacgagca tttgctggcg
     6181 aaggtttcgc tggcgaagga aaccaagtcc aaggcgccga tcaagacctg gtcgcggcgc
     6241 tcgatgatta cgccggactt tgtcgggctg acgttcaacg tgcataacgg caagatattt
     6301 aatgcggtgt ttgtgaccga gaacatggtg ggccatcggc tgggcgagtt ttcgccgacg
     6361 cgcactttcc ggaagcacgg cgcgcatacg gccaaggcgg aagcgaagta atgctatgga
     6421 agttcaagcc ataaccagga acgtgcgcat gtcggcgcag aagatgcgcg aggtggtgcg
     6481 ccagattcag gggctgccgg cggtgcgggc gcaagcggtg ctggcggtgg tgccgcgcaa
     6541 gggcgcccgg ctggtggcca agacgctgcg ctcggccatt gccaatgccg cggatctcaa
     6601 ggagcacaag aaggaatatt cggggttgcg cgtggaccgg ctggtgatta aggaggcgct
     6661 ggcgcaaacc gcggcctcct acaagcggtt tacgcccaag gcgcgggggt cggcggggcc
     6721 gattatcaag cgcaattgcc acatcaagat tgttttgtca gacgaataaa tatgggacag
     6781 aaaactaatc cggttggcct gcgcatggcc gttaaccatg agtggcgttc ccggtggtat
     6841 gccggcaaga aggattttgg cgcgctgctg agcgaggatc gcaagattcg cgcgctgctg
     6901 aagaagaagc tggagtccgc ttcggtgccg caaatccgga tcgagcgggc ggcgagccgc
     6961 tgccgcatta ccattatgac cgcccggccg ggcattgtca ttggccgcaa gggagcggag
     7021 attgacaaga tcaaagagga tttgagcctg atgaccggca aggagattta cgtggatatc
     7081 gaggaggtga aatcgccgga gattgacgcc cagctggtgg cggagaacgt ggcgctgcag
     7141 ttggagcgcc gggtttcgtt ccggcgcgcg atgaagaagg cgttgcagac ggccaaggat
     7201 ttcggcgcgg aggggatcaa gattcgctgc gcgggccgcc tgggcggcgc ggagctggcc
     7261 cgggtggagc agtatcactg ggggcgggtg cccctgcaca ctttgcgggc ctcgattgat
     7321 tacggttttg cggaggcgaa cacgctttat ggcaagctgg gaatcaagtg ctggatctgc
     7381 aagggggacg tgaaggcaga aaaagccccg gcgacggcgt cggcggcggg cgcaaattaa
     7441 gaaggatacg aggactggat tatgccattg atgcccgaga gagtgaagta ccgcaaaatg
     7501 caccgcggca atcgcgccgg cttggcgacg cgagggcaca cggtggcttt tggcgagtat
     7561 ggattgatgg cgctggagcg gtgctggctg gatacgaagc aaatcgaggc cgtgcgggtg
     7621 gcggtcgccc gcaatatgaa gcggcacggc aagatgtgga ttcgtgtttt tccccagaag
     7681 tcgtatacga agaagccgct ggaaacgcgt atgggcaagg gcaaggggcc ggtggagtcc
     7741 tgggtggcgg tggttcggcc ggccaacgtg attctcgagg tggacggcgt ctccgagacg
     7801 gtcgcgcggg agtcgctgcg cctggcggcg acgaagctgc ccatccggac gaagtttatt
     7861 tcgcggcacc gccgggtttg acgcgaggcg cggagcaggc gaggcgccgt agatttggtt
     7921 atgaaaaagg ctcaacggaa agagattcag gaaaagacgc tggccgagtt gctcgcggaa
     7981 gcgcggaact ggcggcaaga gatattcaac ctgcgtttgc agcaggccag cgcccagctc
     8041 gagaagccgg cgcgcctgcg gaccttgcgg cgcgatattg cccgcattga aacgcgtatt
     8101 tcgcaactgc gccgcaaggc ggcctaagga tttggtatgg ctgaaactgt gataaccgcg
     8161 gaaccgggtc gggctcaacg caagcagcgg gtgggcgtgg tcattgcgaa caagatggcc
     8221 aagacgatta cggtggagat catgcggcgt tacccgcatc cccagtataa gaaagtcgtc
     8281 accggctaca aaaaactgta tgcccacgac gaaaagggcg aagccaaggt gggcgaccgg
     8341 gttcgggtgg aggagacccg tccgctttcc cggaccaagc gctggcgttt ggtggaggtg
     8401 gtggagcgtg gttccggcgc ggcgccggtg gcggccggat aagccggttt tctcccgctt
     8461 aaagcaggaa atagattatg ttacaagtac gatccatctt agacgtggcc gacaataccg
     8521 gcgcccggcg agccgcggcg atcggggtgt tgggccgcaa ccagcagtac gcgcgcgtgg
     8581 gcgacgtgat taaggctcat atcaaagagg ccgcgccgga tggcaccgtg aaaaagggag
     8641 aggtggtgga tgccgtggtg gttcgcaccc gccaggtgat ccgccgcaat gacggctcgt
     8701 atttgcggtt cgatagcaac gcgattgtct tgattgacaa ggagttcaat ccccgtggca
     8761 cgcgtatttt tggccccgtg gcgcgcgagt tgcgggacaa gaagtttatg aagattattt
     8821 cgctcgcgcc ggaagtgatt tgaacggcca gcaccagttt tatgcgcaag tgtcatatca
     8881 agaaagggga tgaagcggtg gtcatcgccg gggtggacaa gggccggcgg gggcgggtca
     8941 ttgccgtgat cgccaagaag cagcgggtga ttatcgaggg ggcgcgcatg atcaagaagc
     9001 acatgcgcaa gagccagcag tatccgcagg gccagatcgt ggagcgggag ggctcgatcc
     9061 atatctcgaa tgtgatgaag gcggagaaat atgatgcgcg ggccgcccgg cgcggggctg
     9121 cggcgccggc tcggacgtga ggctttagga ggatacggca agcgaaatga aagccagact
     9181 gtacgaaaga tacataaaag aggtggtgcc ggcgttgcgg gaaaagcata agcacgccaa
     9241 tccccaccag gttccccgga tggagaagat tgtcatcaac atgggggtga gcgcctcgtt
     9301 ggaaaagagc gcggtggatg acgcggccaa agacctggcg ttgattacgg ggcgcaagcc
     9361 ggcgatcagc aaatcgcggc ataacattgc caatttcaag ctgcgccggg gcatgcccat
     9421 tggctgccgg gtgaccttgc ggcgggacgc gatgtatgag tttttcgatc gtctaatcgc
     9481 gacggccctg ccgcggattc gggattttcg gggtctttcg ccccgctcct ttgacgggcg
     9541 gggaaattac tcgctggggg tgggcgacca gacgattttc cccgagattg agctggacaa
     9601 gattaagcgg cagcagggca tggatattac gatagtgacc agcgcccgga cgaatgagga
     9661 agcctttgat ttactgaaac tcatggggat gccctttgcc gaggcgcggc aggcggaggt
     9721 tcggcgcgcg cccgctccgg cgagcgaggc acagcaagat aaaactgagc aaacttgatt
     9781 atggccaaga cagcatggat tgaacgcaat aagaagaagg cggagacggt taagaagtac
     9841 gccgcgatac gagccgagtt gaaggcccgg cgcgattacg ccgggctcgc aaagctcccg
     9901 cgcaatgcca gcccctcgcg ggtggtcaac cggtgttcga tgagcggccg tcggcatggc
     9961 tatttgcgca agtttggctg ttcgcgattg acgttccgag aatcggccct gaacggtttg
    10021 atcccgggcg tgaccaaagc cagttggtaa atctatgaac gatccgattt ccgacatgct
    10081 gacccggatt cgcaacgcca gccgcgcgct gttgccggcc gttgaagtgc cccattcccg
    10141 gatcaaggag ggaatagcgg gcattctcaa gcgggaaggc tatattgccg actttgcggt
    10201 ggagcgaaag acgcctcgga cgctgacgct gaagctgaag taccagggca aaaaaagcat
    10261 tatcgagggg ctccgtcgag ttagcactcc cgggttgcgt cgttacgttg gagcggctga
    10321 aatcccgcgc gtgcgtggag gcttgggcgt ggccgtgctt tccacttcgg aagggttatt
    10381 gaccgacgtt caggcccgcc ggaagcggat cgggggcgag ttgatttgtt acgtttggtg
    10441 agaatatgaa atgtcgcgca ggagcaaggc tgcatgtgag caggtctggc cggcatggcc
    10501 ggttggaggc ggatgcctgc gcgcgataac agtttgattt tatgtcacga attggaaagc
    10561 tcccgatcgc cattcctccc aaggtgaagg tggatattaa gggccagcaa gtttttgttg
    10621 aggggccgaa aggcaaactt aattgggaac tgccgcgccg caccggcctt aaagtggaaa
    10681 acggcaaagt agtggtcagc cgcgagagtg atgacgcgcg cgtcaaggct ttgcatggcc
    10741 tgagccgcgc cttggtcaac aatatggttc gtggcgtcag cgaaggcttc gtcaagaaac
    10801 tcgagatcca gggggtcggt ttcaaggcgg cggtggcgga caataaggtc accctgaacc
    10861 tgggttactc gcatcccatt gtttatgcga ttcccgccca gatcaaggtg accgtggagg
    10921 aaaacacccg gctgaccatc gaggggcccg accgccaatt ggtggggcgg gtggcggcgg
    10981 agttgcgaag cttttatccc ccggaaccgt acaagggcaa gggggtgcgc tattccgatg
    11041 agcatgtcat ccgtaaggaa ggcaagacgg tgcagtaatg cgcggggcgc aaacgtgagc
    11101 gatgagagcg ggatgcttga tggcttttga cccaatccgc gacaaattaa gatgagaaca
    11161 gaacagaaac aaagactggc gcagcggcga cggtggcgca tccgccggaa agtcagcggc
    11221 acaaaggagt gcccgcgcat gagtgtttgc tttaccaaca agaatattca tgtgcagttt
    11281 attgacgata cggcgggggt gacactgggg gcggtttcga cggtcagcaa agcggtttcc
    11341 cgagcggaga aactggcggc taatgctgtg agcgcccgga agattgggga gcttgccgcg
    11401 caaacagcgt tggataaggg catcaagcga gtggtgtttg accgagggag ggcgcgttac
    11461 cacggcaaag taaaggcctt ggccgacgcg gcgcgtgaaa agggtctgca attttaaagt
    11521 aaaacatgga aacgaatatt gtggccgaac cagctaaagt cgaaggtgcc gggccccgag
    11581 cgccacgcgg gcccgggcgc ggacggcgga gtcgtccgga acccgaaccg acttttgatg
    11641 aacagtccgg gcaggaactg accgagaagg ttgtgtttat taaccgctcc gccaaagtgg
    11701 tcaagggcgg gcgccgtttt aattttagtg ccttgattgt tgtcggggat cggcatggcc
    11761 gcgttggggt cggcctgggg aaagcgggag aggtggcgga tgcaattcgc aagggaggag
    11821 aaatggcgcg gtcacagatg atgacggtgt cgttgaaaga ggccacgatt ccgcacgagg
    11881 tgtactcgca ttattgcggg gccaaagtgt tgcttcgccc ggcatcgccg gggacgggaa
    11941 tcattgccgg caagacggtg cgggcggtgc tcgaatcggc gggcgtcaag gatgtcctga
    12001 ccaaatccct cggctccaaa aatgccgcca acgtggttaa agccacgctc aatgcgttgc
    12061 tgagtcttcg gctgcgtgaa gacatctatt cggggcgcgg cttggaaatc aagaagacgc
    12121 cgctcccgca ggaaccggag ccgttgtccc cgctggcgcc gcctgctgat tccttaacag
    12181 cctgattttt tatgcgatta cataatctcc aacctcgacc tggtgcgcgg catcgccgaa
    12241 aacggctggg gcagggcgaa gccagcggcc atggcaagac cagcggccgg gggggcaaag
    12301 ggcagaccgc ccgttcgggc agctcgattc gtattggttt tgaaggcggg cagatgccgt
    12361 tgatccggcg cattcccaaa cgcggattca ataatgcgcg acatacgaag cggtatttgc
    12421 cggtgaacct cgaatctctc aaccagtttg cggatggcac gcgtgtggat ctggaatcgt
    12481 tgcgaagcaa gggattggca aatggttccg ccgccggcat aaagattctc gggagcgggg
    12541 aattgacacg gaaactgacc gttagcgctc atgccttcag cgcgtctgcc agggcgaaga
    12601 tcgaagcaaa agggggcgcg tgcgagctca tcgctccgaa aggggcgaag cgggcaaatc
    12661 ctgaagccgc gaagcggtaa ctggttcatg ggtgccatcc cgcattccaa tccggtcaat
    12721 aggccttggg atttcagccg gcaaaagcct tcccgatttt gatcaaccat gcttgtcgct
    12781 atctacaata cctttcgtaa ctgcttcaag attccggagc tcaaatcgag aattctgttc
    12841 accctgatgg tgctggctat ttgccgactg gaagcggtgg tgcgcattcc ggggctagac
    12901 ggcacaacct tggcgaatta cttcgccagc aaggctggtt ctgcggaggg agcatccgta
    12961 ttgggcatgt acagtctgtt tacgggagga gccttggaac attgcgctgt cggagccttg
    13021 gggattatgc cctacatcag cgcaaccatc attattcagt tgttgacggc ggtgatcccc
    13081 actttgagca aactggcacg ggaagagggg gggcgcacca agattgtgca atatgggcgt
    13141 taccttaccg tgattctttg cctgggacag gggatggtaa tggcgatagg gtgggaaaac
    13201 ccggagaaac tgtttccggg gtggaacacg gcagaccggt tggtgctcta ttccgatgcg
    13261 catatctggt tctatcgcat tcagaccttg cttctgctga ccacgggcac aatgctgctg
    13321 atgtggctgg gagagcaaat taccgaccgg ggcattggga atggagtttc gctcgtcatc
    13381 accatcggca tcctggcgcg ccttccgcaa gcaatggtgg cgctcaagga tatgttcttc
    13441 cccggcggcg ggatggaggc caagttcaat tttggacatg cgatcgcgct ggtgcttttg
    13501 ctggcggggg ttattgccgg cgttatcgcc atcacccagg cccagcgcaa agtgccggtc
    13561 caatacgcgc agcgcgcggt ggggcgcaag gtttattcgg gtggaacttc gttcatgccg
    13621 ttacgggtca actactcggg ggtcatgccg attatttttg cccaggcgat tctgatgttt
    13681 ccgcaaaaga tatttctatt tctcggccag agcctgaacg tcaagttctt tggagagatc
    13741 gcccgcaact tgggcgaggg ggcgattctt tatatgaccc tgtatgggtt gatgattctg
    13801 tttttctcgt atttctgggt ggcaacgcag ttcaacgaat tgcagattgc cgacgatttg
    13861 aagaagaatg gcggctatat accgggggtt cgtccggggc aggcgacgag cgattatctg
    13921 cataacgcga tgagtcggat tacgctggct ggggcgattt tcctgacgat tatagcgttg
    13981 attcccattt ggctgtggcg ggaactgcaa attccttatt cggtggcaac gttctttggc
    14041 ggcacgagca tgctgatctt ggtcggcgta ttgctggaca ccatgcgcca gacggagacc
    14101 catttgttga tgcgccatta tgacggtttt ctgaagaagg ggcggctccg gggtcggttc
    14161 tgatgatcat tctaaaaagt gagcgggacc tggaagcgat gcggccagct tgtgtggtgg
    14221 cggccaccgt gctgaatgac gtggctgctt ttattcaacc cggagtttca acgaaacaag
    14281 tggatgagtt tgccgcgtcg cggatcaaac aacttggcgc gcggagtgct tttttggggt
    14341 atcggaagta tccgtgccat acctgcatct cggtcaatga tcaagtggtg catggtttag
    14401 cgggaaatcg ggtgctccag ttcggcgata ttgtaagcct ggatgttggg gttattcaca
    14461 atggatttat aggcgatacg gcccgaacac tggctgtcgg cgggtgtggg gtgttagccc
    14521 aaagactgat ggatgttacg gaaaaagggc tttatctggg gatagcgcag gcgcgggcag
    14581 gcgggaaggt gtctgatatt tcccgggcga tacaggatta cgtggaaggc aacggtttta
    14641 atgttgtccg ggagtttgtg ggccacgggg tggggcgttc catgcacgag gatcctcaga
    14701 taccgaactt cacggatggg aagccttccc ccaaactgcg ggccggcatg acgttggcaa
    14761 tcgaaccgat ggttaacgcc ggggggcctg aggtaaaaat gttgaatgat ggctggacag
    14821 cggttactaa agatggttca ctatccgctc atttcgagca cacagtattg gtgactgagt
    14881 ccgaaccgga gattctgacg tgcctcgaaa aggtgtcctt gaagtagaag gggccgtggt
    14941 ggaaatgctg ccagacaaga tgtgtattgt cgaactggcc aacgggcacc ggatgcgggt
    15001 gtatgccacc cgcaaggccc gattgcactg ggttcccttg gcccttggga accgggtcca
    15061 attgaaagta tcgcctgggg atttgtcatc cgggcggctg atagtggaaa agcaaacgag
    15121 ttaaatatga aagtccgtgc atcggttaag aagctttgcg agaattgtaa aataattcgt
    15181 cgccgggggg tcatccgtgt aatctgctcc aacccgcgcc acaaacagcg acagggctga
    15241 ttaatcaacc atttaattta tttataccga ctaagattta tggctcgtat cattggcgtg
    15301 gaagttcccc caaacaagcg cattgatatt gcgcttcgct acctctatgg cctcggcccc
    15361 agtaattcca gggctattct ggccaaggcc gggattgatc caagtatccg cgccagggat
    15421 ttgacggagc agcagctttc gcaaattgtg cacgcgatac aggacggcaa atacgttatt
    15481 gaaggtgatt tacgccgtga aattggcatg aacctcaagc gcttgcaggg gattaaatgc
    15541 tatcggggaa ttcggcactt gcgcgggttg ccggttcgtg gccagcgcac ccagaccaac
    15601 gcacgcactc gcaaggggcc gcgcaagaca gtgggtgttg ttcgcagtcc aaccgcaaaa
    15661 tccggcatcc attaacgatt ttccgctggt ttatctatta accgccaatt gatcaactaa
    15721 ctcatggccg acgagaccaa aaagaaatcc acccccctca gcgagggtaa acccgggggt
    15781 aaaaaaccga tgactgaaga gaaggctgtt gcggcaaagg ccggggaaaa atcaaccgcc
    15841 caggctgaag cggctgctcc ggcggctaaa gcacgcaaga aagcggaccg taccacggag
    15901 aataccgcgg cgcccgccga agctcccaag gcagcagaag gcggcacgcc cgtggcggcg
    15961 ggggcggctc ctactgcggc cgaattgctg gctgacgatc tggctggcaa gaagattatt
    16021 aaagccaagg gggctaagaa tatcgtcagt ggcattgcca atatcctagc gaccttcaac
    16081 aacacattgg ttagcatcac cgacatgcag ggacatgtga ttggctggtc cagcggaggg
    16141 cgcgttgggt tcaagggttc gcgcaaaagc accgcctatg ccgcccagca ggttgcgcag
    16201 gatgccgctc ggcaggcgat gtctcatggt atgcgcgaag ttgaagtgcg ggttaacggt
    16261 cccggttcgg gacgcgaatc ggcaatccgg gctttccagg ccatcggatt ggagattacc
    16321 gccatcaagg acgtcactcc cgtgccgcac aatggctgcc ggccgcgtaa gaaacgccgt
    16381 gtctaatcct taaccacaac gcttaacttt cctctctaaa aatggctcgt tatactggtc
    16441 ctcgcgttcg tatcagtcgc cgtttcggtg ttccgatctt tggcccctcg aagtatctgg
    16501 agcgccgcaa ttatggcccg ggagtacatg gcccgaaatc acgtcgcaag cacacggatt
    16561 atgcgctggg gctgattgaa aaacaaaagc tccgctatta ctacggcctg ctggagcgcc
    16621 agtttcatgg tgtttacgaa cgcgccctcc aacgccgtgg gatcaccggc gagcacatgc
    16681 tgcagatttt ggaaactcgc ctggacaatg tggtgtatca tttgggtttt gccaacacgc
    16741 gcgccacggc gcggcagttg gtggcccatg gtcatgtgag ggttaacggc cgcaaggtta
    16801 gtattccgtc gtttggcctc aaagttaacg atagtgtgga agttaaaaac tccacggtat
    16861 cgcgccagtt ggcatcgcgg aatctggaaa gtgctgccag ccgggctgtg ccggattggc
    16921 tttccctgga caaagaagca ttcaagggtg tcgttatgcg aattcccacc cgcgaagaga
    16981 ttcagccgat tgccaacgag caggcggttg tcgagtttta ttcccgttaa aaagtgaaac
    17041 tggattgtag gatggttgaa atagctgcgc ggctacgagg gcctaaccgc tcgggtcggc
    17101 aggcgcctgt tccagccctc cgtcgcaatt atacggactc cttcggccct cgtgaatagc
    17161 ggttgagggg tcagattaaa attgttgaaa ggaaaatatg ccagtacgtt taggacgatt
    17221 tgaaatgccc aagcggttgg tcaaagaaga tttgaccgcc accgccacct atgcccggtt
    17281 tgtggccgaa ccctttgaga ccgggtatgg ctacactatt ggcaattcgc tccggcgcgt
    17341 tttgctttcc tcccttgaag gcgcggccat ctcctcaatc aagattacgg gagcgatgca
    17401 cgaattcacg atcgtggatg gggttgtcga agacgtcacc gagattgtgc taaacctgaa
    17461 gaaaatacta ttcaaggcgc acacccgcga tcctcagact cttttcctct ccgttaaccg
    17521 ggagggtgcg gtcaccgccg ccgacatcca gcttaatcag aatctggagt tagttaatcc
    17581 ggaccagcat atctgcaccc tcgacaagaa gaaaaaactc gaaatggagt tggaagtcaa
    17641 aatcggtcgc ggcttccttc ccggagaaga gaacaaaaga acgaatcaag ccattggagt
    17701 catagccatt gattcactgt tttctcccgt tactcgcgtt cgctatgcgg tggaaaacgc
    17761 ccgcgtcggc caacgcaccg attacgaccg ccttattttg gaaatatgga cggatgggcg
    17821 catttctccg gaagacgccc tgacccaggc atctgccatc cttcagcatc atttggatat
    17881 ctttgtcggt tacgacaaaa acgctgttga gtttgaggaa atcgtggata agcaggatga
    17941 agaaaagacc cggcttaaga aactcctcaa tatgagcgtc aacgaaatcg aattgagcgt
    18001 tcgtgccgcc aactgcctga ataacgcaaa cattaccacg gtcggccaat tagcgatgaa
    18061 gaccgagcag gagatgttaa agtatcgcaa ttttgggaaa aaatccctca atgagatcaa
    18121 agaaaaattg gcgggcttga atttaactct cggaatgacg ttcgaggcgg aactgctgga
    18181 ggccaccaag gagaagccgc tcgttttgcc aaccggtcgt tagcctggct gggggaagcc
    18241 ggccggatgg cctcctgccc ccttgttgat cgaacattca tgactcgctt ctatttatga
    18301 gacatcttaa gcgaaccgcc aaattgggac gcaccggcca acaccgaaat gcgatgcttg
    18361 ccaacctcgt ctgtagcctc attaagcaca aacgggtgat cactacgctg gctaaggcga
    18421 aagctgcccg ctccgtagcg gagaaaatgg tcaccttagg caagtgcggc actcttcacg
    18481 accgccgtct cgccgcagcc cggctccatc aggaggacgc cgtaaaaatc cttttcaatg
    18541 aaattgctcc cgcgcaaaaa gagcgccggg gtggctatac ccggattatc cggttgggcc
    18601 aacgccaagg cgatgccgcc cagcgagcca ttttggaatg ggttgagttg gggacagttg
    18661 ccggaactcc agccaaggaa tcggcttcga ctaagaaagc gaaacccgtc gaaaccaagg
    18721 ttgattccaa agccgaatcc gagaaatagc tcccgcggga gcttgtttga ctctgcgtgt
    18781 tgatgtgggc ctcttagggc cagaagtaac gcgcaaagca aatccctgcc atcgctatgt
    18841 ccagacgccg gcaatcttat taccgcagga cgggcatttg ccattggtca gacggaatga
    18901 ggtaacagca tagatgtcgc gctcgatgac ggcctgcttg cagttggggc accatgttgt
    18961 ctccgcatcc tcgagcccgg gcacattgcc gatataaaca tagcgcagac cttcgctgcg
    19021 ggcgacgttg cgcgccgtaa ccaatgtctc aactggcgtc ggcgtgaggt aggttagttt
    19081 gtgttgcggg tggaagcggg agaaatgcag aggctgatcc ggtcccagtt ctttgctaat
    19141 ccaggcgcac atgcgccgga tggtgtcgat ttcatctgta taggtcggta ctaccaggtt
    19201 gatgatctcg aaccatatcc ccaggtctcg cagcgttttc agggtattga ggatgggctg
    19261 gagcttgccg gagttaagtt tggtgtaaat gttctggtca aatcctttca gatccacgtg
    19321 tgcggcatcc acgtattgat acagcgcgcg agccgcgcgc tcctcgatag agccgcaact
    19381 aaccacaata ttcttgagct tgtgttcccg cgccgctttg caggtgtcga gggtgtattc
    19441 gtaaaacgca gtcggctcgg agtaggtgta ggcaatcgag ggacaatcca gcgccttggc
    19501 caccgccgga gcatcctcgg gaaacaggct taagcgtgcc atctggctca gggtgaaccc
    19561 ggcgggcagg ggcggttgca cgcgcaactc cgggccgtgc gcatccttgg tttcttccgg
    19621 ttttttctgg gaaatctccc agttttgaca gttgaggcaa cggaaaacgc acccggacgt
    19681 ggcgagcgag aaggtacgac tacccggcag gaaatgaaac aacggcttct tttctaccgg
    19741 gtcaacgtgg aacgtgcagg ggttggcata ggccagcgtg tacaggatgc cgtcgcaatt
    19801 cactttgttg cggcaatggc tgcgatcccc cggtgccagc aggcaattat tcgggcagac
    19861 cttgcactgg acgttgcgcc ctaacttgag atggtgcgat gcttccttta cccagccccg
    19921 tcggcgccac agttcccaaa gctcgccttt gggggcgtca cccttgaaga tatccgccgg
    19981 catttcggcc tcacgccagc gggtgaagaa atcgaaccag gaagcccccc cggcaagact
    20041 cgcggccccc gccacgccaa ggccgatagc tgcgcggcgg gagaatactc gctgcccgga
    20101 cttttgccct tcggctttcg cccttttccc aaacgctcgc ctttgcgctt tgaacttgcc
    20161 gttcatgatc gtgcgcgagg cctgatccag ccccgacgct gcagggcaag atacgccgcc
    20221 ggcaggctgt gcgccagtag cagtacgaaa aacggtaaat gcagcatccg atggagcgtc
    20281 agggccgatt tctcattcat caaccggttg agcccgtaac ggccatctat aatgtagccg
    20341 gagaacggga aaagtagtgt aataagcagc agcggccaac cgctccatcg gacgatctta
    20401 aggaggatga ggctaagtct gttcatacga tgcttgttgg aaagcattgt ctccggaagt
    20461 gaatatcgca agtcaatgta atcccctcgg ttatgttagg tatattggag agcatctccg
    20521 tttgtgcgtt tcacgcccag aaaatctttg ttctggcgct accttggttc gggtttgaga
    20581 tgggtgttaa tggcagagac gggcggggag acagtagaac ttcgtctctc cttttcttgg
    20641 cgacgccatc cgcgcgattt cctcttattg tcacaagtat gaagatgaga attgtttagc
    20701 tccggaaatg gcttggattt atagagtgaa acactgcaac tcattctaat acgtacactt
    20761 cctccaacct ccaatcgggg caagccaaga gcttcgtatt cgccgataac gatatgaata
    20821 atccgcgttg tctacctttg gcatactcaa ctgagcctgt cactgtttgc gggcgataat
    20881 tggcggcctg tgggagtgag tcgccgggtt ttctctcgcc cccggctatc ttcaataata
    20941 accaccgaat cgttaaggaa gaaaccccgc agggcggagc gaatcgacac tgttttgcgc
    21001 acgttaattg gccatgaatt ggggttgccg catctgtaaa tgttgcacta ctttgctcct
    21061 tcgaatcttt agcaaaagtt gtttatggac aagactgcga tgctgacact ggacggaaag
    21121 agctatacgc tcccgattat agcgggcagt gagggagagc gggccattga cgtttccacc
    21181 ctgcgcaacc aaactggctg catcacgctg gacgaaggtt atggcaacac gggctcctgc
    21241 cgcagccaga ttacctttat tgatggggag aaggggattt tgcgctaccg cgggattccc
    21301 atcgaggaac tggcggagaa atccaccttt gtcgaggtcg cccatttgtt gattatgggt
    21361 cggctgccaa acagtccgga attgcggtac ttttcggatc tgctgaccga caacgagttg
    21421 ctggacgaag ccatgaagca ccacttcgag ggcttcccgg cgcatgctca cccgatggct
    21481 attctttccg ccatgatcaa cgcggcgagt tgcttccacc ccgaattatt gaccgatcgc
    21541 caacacgact gtttcctggt ccaggcagca cggctgcttt ccaaggtgcg gacaattgct
    21601 gcgtttgcct accgaaagtc gcggggtttg ccggcgattt atcccaagcc cgagctcaaa
    21661 tacaccgcga attttctgca catgatgttc tcgctgccaa atcgggacta tgcgctcacc
    21721 ccggaggtag ttcgggcgct ggacctgctc tttctgttgc acgccgacca cgaacaaaat
    21781 tgctcgacgg ccacggtgcg catggcggcc tcgagccacg ccaacttgtt tgccagtgcg
    21841 gcggcggggg tttgttcact gtgggggccc ttgcacggtg gcgccaacca agccgtgctg
    21901 gagatgctcc aggcaattca ccaggcgggc gatgatggct caagatttat cgctgcggcc
    21961 aaggacaagc atagcggcaa gcggttgatg gggttcggcc acagggttta taagcattat
    22021 gatccgcggg caaagatcat caaacgcgcc tgtgatgagg tgctggccga actccacatt
    22081 agcgacccgc tgctcgacat tgccaagcac ctcgaggaag ccgcgctgcg cgacccctat
    22141 ttcatcgaac gaaagctcta tcccaatgtg gacttttaca gcggcatcat catgcgcgcc
    22201 attggcatcc cggtcgaaat gttcacggtg gtttttgcca ttgggcgcat gccaggctgg
    22261 attgccaact acaaagaggt aatggaatct tccgcccgca tttgtcggcc acgccagatc
    22321 tacaccgggc caacgctcga ccattatacg cctctcaagg aacgcaaatg aggctcgctg
    22381 gcttcgggat aagtaatcag ttcaggcagg aggctgcggc accacgctca tgaacctaca
    22441 cgaataccag gcccggcaat tgctggatca ggcgggagtg gcgcaagcgg cgtgggagtt
    22501 ttgtgctacg ccagaggcgg cccgggatat tgcggcgcgg tttttgggca agggagccac
    22561 gctcatcgcg gttaaggcgc agattcatgc cggggggcgc ggccggggta cgtttaagaa
    22621 tggttttcaa gggggcgtca ggctttgtcg aacggcggaa gatgtttacg aaaaagcccg
    22681 ggcgatgctg ggcaatgtgc tggtcacccg gcagaccggc cccgaagggc ggctcgtgga
    22741 caagctgatc atcgcggttg cgccggacat ccggaaagag ttttacctgg ccgtgctgtt
    22801 ggatcgggtg gcgtcccgcc ccgtggttat ggccagcacc gagggtggca tggatataga
    22861 ggaagttgcc gctcaaaccc ccgacaagat catccgggaa cacattgacc cggctatcgg
    22921 catgatgccc tatcaggctc gcagggtggc cgtcgctctt ggcttgcggg ggaatttaat
    22981 cgctcaggcg gtgaggctgc tgctgggcgt ttaccggact tggtgggaat gtgacgccac
    23041 gcttgtggaa cttaatccgc tttgcattgt taagaccccg gacggctccg agatgctcca
    23101 ggccgtggat gccaaactct cgctggatga caatgcgctc tttcgacacc cagaactgct
    23161 ggcgatgcgg gacctggcgg aagaagcccc gctggaagtc gaggccagcc ggtttaatct
    23221 aagctacatc aagctcgatg ggaatatcgc gtgcctggtc aacggggccg ggctggcgat
    23281 ggccaccatg gatgttatcc ggcactacgg cggacacccc gccaactttc tcgatattgg
    23341 cggcggcgcc agtcgggaac aggtggcggc tgctctcgaa attgttctgc aggacgcgag
    23401 cgtgaagggg atcctcgtca atattttcgg aggcatcatg gactgcgaca ttatcgccaa
    23461 cggaattgtc gctgctgcaa ggcaaacggc acgcaagcta cccgtggtga ttcgtctgga
    23521 aggcaacaac gtcgaggcgg ctcgaaaaac ccttgctgaa tctggccagg cctttatcta
    23581 cagcaactca atggacgatg cggcgcaaaa aatagtcaag gccgtaaatt ccccgtagcg
    23641 ctgcctcaca tgtctatcct cgtcaaacca gatacgaagg tcatcatcca aggcatcatg
    23701 ggccgttttg gatcacggca cgcgcaactc agcctggcct acggcactca aatcgttgcc
    23761 ggcgtttccc cagggcgggg agggcaacgc tttgccaacc aggttccgat cttcgataca
    23821 gtgaccgagg ccgtgcagca gaccggcgcg accacctcgg cgatcttcgt ccccccgcct
    23881 ctggcggcag atgcgatcct ggaaggcgtg agcgccgggc tggacctggt cgtctgcatc
    23941 acggaaggca tccccgtcaa ggacatgatc aaagtgaagc gagccatgac aggcaagccg
    24001 acgctgctta ttggcccaaa ttgcccgggc atcgtcacgc ccggcggcag tcaggattca
    24061 ccccgcggct gccggattgg cattggcccc ggctacatct atcggccggg ctccatcggc
    24121 gtggtctcgc ggagtggtac gttgggctat gaggcagtat ggcaactcac ctgtcgtggt
    24181 cttggccagt ccacctttgt tggcatcggc ggagatccgg ttcacgggat ttcgcatttg
    24241 gacgccatca aacgtttcgc ggcagatccc gagacgcagg gaatcattat gattggtgaa
    24301 attgggggga cggcggagga ggaagcggcg gcctggatcc gggataactg ccgcaagccg
    24361 gtcgtcggct ttgttgcggg ctcaacggca cccccaggcc ggcgcatggg ccatgctgga
    24421 gcaatcatta gcggcgggca aggcacggca gcggccaaaa tttctgcctt tcgggaggcc
    24481 ggaattggcg tggccatgac ccccgcggaa atagtggaca ccttggtgag aatgctgtga
    24541 agtggtgcgg gttgcggatt gcgttaggag ccgaaaaagc gcaatatcgg gttcgcggaa
    24601 cacgcaatac atgacacgct cagatgggtc tcattgattt ggtattgaac ctggctggaa
    24661 cgctgctctg gctcgggtgg cgttccatgg gcctggatcc tctggttaag gccacgccgg
    24721 tatccctggt cggcaccctg cggcgcgcgg aacctcgccg gcttagaggc tggcagttgc
    24781 tggtgggcct gaccgcgctg cttctgttgc gggcggcgct ctattgccag atcgggcccg
    24841 aggtgaattg gacccccaaa ctcaacctct tctttgtggt gctggccttc cacagtcacc
    24901 tcttcctccc gtctctgctc tattcgatac tgagtttcgg acgccttcta attatctgct
    24961 acttctggat gttggtcctg gtaatcatca accgccgaac cggatccccc gatccggtct
    25021 tgaaggcgct ccgcgcgcac ctgagcgcgg tcgcacgctg gccgcgattg ctgcaatggg
    25081 ggctgccgct tctgtcagtt gccgccctgt ggatcgcctt gcatccgtta ttggtgcaac
    25141 agggaataga ccaaccggcg cagacgcatg cccggctggc ggggcagggg atgctcgttg
    25201 gcgcggctct ttacctgagc ctccaatatc tgctgccggg gtttctcttt gcgcacttgg
    25261 tggcgagtta tgtgtatttg ggcgacagcc ctttatggga ttttatcaac gctaccgccc
    25321 gcaatctttt ggcgccgctt cgacggttgc ccctgcaaat cgcccacgtt gatttagctc
    25381 ctgtggttgg agtagcgctg atttttgtgc tgctgcactg gttgccggat ctcatcgtgc
    25441 gcaaaatggc cctccaccag ataagtttct ggccgcaata accgcttgct cccgggaagc
    25501 ccggcttacg tcgccaccac tttcggcagc ttgagcgtca cggttgtggc ggtgccgcgc
    25561 tcgctctgaa tcgtcagcgt gccgccatgc agttcggcca ggcgcttggc gatcacgagt
    25621 cccagcccca agccctgttg ttcctgcatc ttgcgatcaa attgcatata ggccccaatc
    25681 cgggtgatat gctcggttga aaacccccgc ccccgatccg caacggaaag cgcgacccca
    25741 ttgggcccgt ccgagagagc cacccgcacc gcgctgccgc gctcggaaaa tttgaaggca
    25801 ttctgcacca actcatccac aatcttggcc aggtactctt cggatatcgg cataggcagg
    25861 tccgcgagat tcagccccag atcctccaaa cggtttgcag cccgcgcctg cttccgggca
    25921 cgctcctcaa tgagcctcgc gggggaatcc gtttggttca ggcggagcgc cccgattttc
    25981 tgcgcgtccg tgcccagtaa ctcgatttgg gcgaaaatca ggaagttctc aatcaaccgc
    26041 tccaattgct tgccggagtc gtggatggtc tggcccatct cggcgatttc actggctggc
    26101 agcgactccg ccccggtggc gaggatttcc ccataagcga ggatgccgtt caggggggtg
    26161 cgcaattcat gcggcaacat caggctgatg ctgttgcgca ggtcggttag cttcttctcc
    26221 gcctcctgtc gtaccacttg ggccttctta agccgcgttt cgaccgcggc gtataaggcg
    26281 tcaagcgtga acggcttggg caagtaatca tccgcgccca gttccatgcc ttgccgcata
    26341 ccggcgttgt ccgccgagcc ggtcatcagt ataaaaggaa cagccgccgc gctcggctcc
    26401 tggcgcaggg ctgacagcgt cagatacccg tccatctttt ccatgttgac gtcgcacaga
    26461 accaggtcgg gcagttcccg gcgcgccttc tccactccgg caacaccgtc ctccgcttcg
    26521 atgacttcat agcccttttg ctgaagcgcc tggcgcacca tctcgcgcaa ccattcttcg
    26581 tcgtctatga ccaggattcg tttcatcaac gttgacggca gccggcagcc cctccacccc
    26641 ttctttagag cggagaaaac cgatctctca agcctgagcc acgataagac gtccgcgccc
    26701 gcttgtccac ggacattttg gccgctgcag ccgggcgcgc ttcaatttcc ccaaggcttt
    26761 tcgggtgagc ttaaaacgaa gccaaacgaa gaaagcaaag acaaagccgc acccccgcca
    26821 gatgtagctt ggcgaaatca ttgccgggcc gacccatacc ccacccgtca cattcgagcc
    26881 acccggcagt gcgggcagag gccgcaacct ctgcccgcct caatctactg gcaaacttcg
    26941 cccgccgt
//
LOCUS       DAOPQG010000046        26423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2367_length_26423_cov_14.439510, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000046 DAOPQG010000000
VERSION     DAOPQG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 26423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26423
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2367_length_26423_cov_14.439510"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..351)
                     /locus_tag="PKI07_07070"
                     /pseudo
     CDS             complement(145..351)
                     /locus_tag="PKI07_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(418..6459)
                     /locus_tag="PKI07_07075"
     CDS             complement(418..6459)
                     /locus_tag="PKI07_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOC50853.1"
                     /translation="MSLPLQAGPRDSQWRAVEAAITQGRPRTAITNLEPIIAAALKDK
                     AYAEAVKAIGQKIALEGNLQGNQPEEKITRLEAEIAQAPSEMVPLLETLLAEWYWHYF
                     QQNRWRFMQRTTTGVPPGNDFTTWDLPRLFERIDQLFQKALRAGPLLKATPISAWDDL
                     LPKGSMPDRYRPTLYDFIAHEALAFYASGEQAAARPEDAFEVSADSPIFDPAEKFLAW
                     QAGAAEASQPELRAIQLYQALLDFHRGDASNPAFAHADLERLTWGWNVAFGENKDARY
                     QAALERFIDTHADSDLSALALDCAARLLQQRDDFVAARELALRGARAFPQSPGGKRCR
                     NRVAEIEAPSARITTERVWNAPWPEITVHYRNVETVCFRAIPYDWEALSAEPHYRLVG
                     EKEHREILSRAPALEWSVKLPPTADYHEHTLAVPAPDRLKPGFYFIAASHRPDFGETN
                     NVVSLAQVWVSRLALVTRVRAGRIEGFVLQADSGEPVAGAKVSVWHRDLQGNRITDPI
                     LTTDQNGCFSLEPVSEQFYWFRARHRGQEVATAENLWYYDDSEASPPSPYAKTVFFTD
                     RTLYRPGQTVQYKGICLWVDRAEDNYKVLKDEPLTVIFEDANGKEVARRQHRANDYGS
                     FAGSFTVPRDRLMGRMSLKASGRAPGQGRFHVEEYKRPRFEVVLDAPQSAVKLNETVV
                     LVGHAMNYSGAAVDGAQVKYRVVREVRMPWWWGWGWGGLPSRPGQEIAHGITRTATDG
                     SFKIEFDARPDPKVPKKDEPVFVFQVHADVTDGAGETRSAGRHLQVGYAALEARISAD
                     DWQTDAQPVELKLETRMLDGEPQAATGEVKVYALRAPAKVHRPPLRSPEPRGPFGAAE
                     DADSPALDLSDPSHWPLGRMVASRSFATGTNGTARLAFQLAAGAYCAVIETQDQFGQP
                     VTGRLPVRVLPAGGSRLNLKVPHLLAAPNWELQPGQEFMAVWGTGYKTGRAFIEIEHR
                     QRMIQRYWTPSGVTRQQIRLALTEAMRGGCFVHVTFIHENRAYLESREIRVPWENKDL
                     DLKWERFVSKLQPGGKETWTLEVRGPTPQGQSGGAAAELVATLYDASLDAFAPLAWPK
                     PFSGSFFRSEYTTLRSQFANAFMRFSPVLGRWNLPGEPVEISYRAFPPELSGNLRGYQ
                     YFARSKNSGRASRSGAAIEMKASVPAAMVAGMGSELAFSKPAAAVRNALDQVGEGARE
                     SDAPAPPLQLSQVTARKNLNETAFFFPQLTTDSNGVVRMTFTLPEALTKWRFLGFAHD
                     RALRSGALEDHAVSAKDLMVQPNPPRFLREGDTVEFTVKVSNPTDQPQRGTVRLTFNH
                     ALNNDPADALLGNLKPERDFDLPARESRSYAWRIHVPDGCGFLTYRAVGAAARVSDGE
                     EGYLPVLARRVLVTESLPLPIRGPATNSFTFTRLLESGAAPTRQNQSLVAQMVSQPAW
                     YAVLALPYLMESPYECTEQTFNRLYANALARFIANSNPKIRRLFDLWKDTPALESPLQ
                     RNPDLQAVMLEETPWLRQANQESQARRNLGILFDDNRLAYEQEAALQKLAGQQLDDGA
                     WPWFPGGRGNDYITLYITTGFGRLRHLGVSLDAPPILRALRRLDAGMAERYARIQKGP
                     EPEKHVPSATDALYLYGRSFFRKDLPITPPHRPVIDFFLKQSRKFWLQTDCRQTQGHL
                     ALALHRFGDLDAANASTARDILQSLKERSVRDEELGRFWRDAERSWWWYRAPLETQAL
                     MIEAFDEILTDPPTVEECRVWLLKQKQTQDWKTTKATADAVYALLLRGPDLLSRDALV
                     QMSLGGVDVTPVPGPAGGSTSPGSVAPGRGKDLPAPAVEPGTGFYEYRFAPDQIKPKL
                     GEIIVRKTDAGVAWGSVHLQYLEELDQITASTNTPLKLNKALYTRVNTSRGPVLEPAR
                     GPVHVGDELVVRLELRVDRDMEYVHLKDLRGSGTEPVNVFSRYRFQDGLAYYESTRDT
                     ASHFFIDYLPKGTYVFEYSTRVQLRGQYQTGPAQIQCLYAPEFNSHSQSLPLTAD"
     gene            complement(6711..8585)
                     /locus_tag="PKI07_07080"
     CDS             complement(6711..8585)
                     /locus_tag="PKI07_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOC50854.1"
                     /translation="MTSELAQPASPVGRQRMDADIVCVGFGPATAGFLTTLSRQLLNP
                     DGTPAVESRVLPGAPPQVLCYERADDLSFGVSGVVTRARGLRASLPQLNPREIPMAAP
                     VTEERVVYLLDPLGVSRRSGPLRAADACLRAFRGLLPVRHEAFELPWAPAFLHKTGGL
                     VLSMGQFLQWIGAQVQSSGTVQVWPGTPVAEALIAGQKVEGVRLLDQGVDKQGRPGDG
                     FMPGMDIHAALTVVGDGPVGPVGLQLDARFGLPAGHHIRDWAVGMKFVVDLPADTPLQ
                     PGAVLHTFGFPEPEIFGFFYVHPDRVASLGIFVPSWFDSPARTAYRYLQHWMLHPYLW
                     RYLKGGKLRSWGAKTLGESGRRGEPHLVGDGYARIGEGSGTTNVLTGSGLDEAWVSGV
                     QLAEGVLELLKAGKPFTRQNLEAAYVRRRRASWVEAEARIAGKARDGFQKGVLRGMIG
                     MALAGLTCGRLNLGGEPLRPYQRIPSLEEYYRGRIPAAEIQRLRKECATQGTSLHHAL
                     MERCGWPAIPYDGQLLVSHQDALLLGGKVQAPPGYADHVLFLYPELCERCGTRICIEM
                     CSGQAIAPGAGGVPSFDREKCVHCGACYWNCAHPIPGDPERMNIAFRAGPGGLHSAEN
                     "
     gene            complement(8612..9184)
                     /locus_tag="PKI07_07085"
     CDS             complement(8612..9184)
                     /locus_tag="PKI07_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50855.1"
                     /translation="MKRATEAFVTGNLFEFGLGHVVFARFRGGDAELGVFLVDVYCQG
                     VKDAFYARVSEGRYDMELLEDVLPAADRKAIDPPSARKLVEGAVAYARGFGLAPHPNY
                     KAACRVFGGTNAAESTTSFNFGRNGKPFYIQGRYDSFEKALRMMNHLRARCGNDGFDF
                     LMRASETQNLELERAGFEIRQKIPAPPDRQ"
     gene            complement(9201..11438)
                     /locus_tag="PKI07_07090"
     CDS             complement(9201..11438)
                     /locus_tag="PKI07_07090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOC50856.1"
                     /translation="MSVAADTPAPPALQTLPGDDLRQIMWRFADRYDLAMLVQSSRTV
                     ARGPVARLVAQGARNSHEWTPEKASLLAAFDESGITAAFLDPQHGGFIEGPKNLALAL
                     IAFELSWVDAGAATCSLAGNLGLAPIHERGTPAQRNHYLALAAPPKPGESRAQVRAAF
                     ALTEPIPFVGVETGMLAGRVRVAEWNEGQEPVLQVEKRGRFITNMGFANVVTAAVESD
                     DPRLKGSCMVILEETDPGAWDRGTPTRKLVHQLSSTRDPVLSLRVPASRIIGGYTVKD
                     GVIIPRYSHGEMIEAVFRRTRVTVGLMTSAKLLSAIEPVIMYARRRFRGGQGAPGTPR
                     YELGLQQKEDVLQRLVEVWATGEASASLGFAAARLFDALDPLEKKKDEILAARKLTGR
                     AAFKALAQSQQEAQELLQLDARPADARDESRREALAADPLVQFALLDSQANVLCPACK
                     LWNTGHGANMMREAVSLMGGYGVTEDCPGFLGQKWMDAQLEATYEGPEAVQRLQLSLT
                     MTNELFLAQFRLWIAELARLAAARPGTGACALATAMKLWLWTLEYLQKATDADGAKLY
                     HKSRQGVTFPLADALCWLLAARQFILDVRELEEKGPANPALADGLPGFVNFFADLCQV
                     QSARAAGEVGRICADLVYGFNRHPAWDSASRAACYSAAELESLEGIIPGIDSSARACA
                     DVTEAGEAHPRKAGPCPRADGLETFTRLRAKLDGCLTGSRLAKDRAAEALTKVMIPEA
                     LDYPG"
     gene            complement(12601..13281)
                     /locus_tag="PKI07_07095"
     CDS             complement(12601..13281)
                     /locus_tag="PKI07_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC50857.1"
                     /translation="MRRKSSTARPAARRARILLVDDHPLVRERLAEIINQEPDLMVCG
                     EAQDRAGALAAMAAHRPELAIIDLALKDSDGLELIKDIRLHWPNVLMLVVSMYEESLY
                     AERVIRAGARGYVTKQEATRDILSAIRRVLSGRVYLNENVSARIIDRLADRSVPGAAT
                     PAEMLADRELQVFELLGRGLVVKEIARRLRIATKTVDTYRRRIREKLNLQTSSQLLQH
                     AISWTRRR"
     gene            complement(13278..18107)
                     /locus_tag="PKI07_07100"
     CDS             complement(13278..18107)
                     /locus_tag="PKI07_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOC50858.1"
                     /translation="MKVLIQRLEARLQRLPSFTITAMGLGWVLALGALDCFTPDAASL
                     TLFYLFAVIFTGWGAGKGPAGLVAAVAALSMLTVQWYWHREIPQPGWLLLWNAATRFL
                     AFSVVGCLAAEVARLARHLSQLVEERTAQWKTEVAEHQSTAARLAETLARFEQVINNI
                     TEAFWLSDVSKRQMVYISPGYERLWGRTCAELYREPLSWLAAVHPADREAVSRSAHAD
                     QSTGGYDIEYRIIRPDGTIRWIRDRGFPVRNAQGEVYRIAGLADDITDRKRAREELQT
                     RAAILEGMTEGVVVTDEQGRIVQMNPAAERMWGYQLEEMLGQPADSISVLPEPEKAAV
                     MQEVLAALRTTGSWHGKFTNRHKSGRLVYCDAFLSRVEVAGRTLYVALQQDVSESRRT
                     QEQLQMQARVLESMGEAVLMVDEQGTILLSNPALDAEVGYARGELIGRPLETLSARSP
                     EEFQRMFACFLDQVKTRGFLRDERLVRRKDGSVFEIECRSSGFTLDGRFFMVLVGQDI
                     TERNRAQLALKQSEESLRVFLNAVPQPALLLDREGVILLANPAASVGMDRSPPELPGR
                     NIFDITPPGMLPDWKNSFDRILQTRKPARFEEEHQGRHYIIFFNPVLDAVGNVSRVAI
                     FALDITERKQSETALARKEELYRTLFELSPDGIVAGDAGGTILDANQALCQAFGYARE
                     ELLGRNVRGFAPLEQQPKVEAHLATLRAGRSLQSELWGLRQDGDRRLMALHATPLMLP
                     DGQPGSLAVIRDITETKRASLIRQAFLTLATHLSEVDSPLAAGRAMVTAADQLWQWDA
                     AVLQLYSAERDCLESVLLIDTLDGQRREVSSAGTDSSPLPRMRQIIENGAELILRTPA
                     ELQSADLVAFGDAQRRSASLMYAPLRRKDQTIGILSIQSYAFNTFTPEDLRTLEALAD
                     LCGGALERIRAEQALREAHDQLERRVQERTTALQAANQALNQAHAQLEQRVRERTAEL
                     HAANTALRQSEERYRSLVNNLNVGIYRNTPDAEGLLLQVNPALARILGYDSADEIRNV
                     RIVDTYQDPGQRAALLAELLRAGSVRNYELLLRKRDGTPICASVNVTVHRNARGEVDW
                     LDGVLEDITQQKRIQEALSASEERYRALAESSPDAILIFDRDLKVQYANPAAAAIWRR
                     PVERLLAETTAALFPSETGPCLEALAQDVFGTGQPARAIEAAPFPAGDQWLEVRFVPL
                     RDARGAATAMMGLCRDISKRKQADILLQAQRDLGAGFSLTSDLDVALRHLLDIILRLG
                     AVDSGGVYLLNEASGEMTLAVHRGGSPAFIKAVSSWTPNSPQMQLLREGQPVFCLYQD
                     LPISHTAPRRSEGLRASAFIPLSHNGRIIGALTLASKVADEIPRQTQIVIEAIAAQAA
                     GAIARIKAETDRQRLEGQILEISDRQQARIGQDIHDGLCQHLVSLAFDANSLHRALAG
                     KHRGPASKARRIALYLDHAITEARQLSRGLFPVQLGTEGLPHALEDLASTTGRRFKIQ
                     CHFSPPKSVIAADAVLATHLYRIAQEAVTNAVKHSQARRISIHLDLHAGRLELRIEDD
                     GRGLSAPQPGAGGGMGLHIMDYRARAIGGTFRIGASRLGGTLVSCCAPVGSGGQSPA"
     gene            18285..18914
                     /locus_tag="PKI07_07105"
     CDS             18285..18914
                     /locus_tag="PKI07_07105"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOC50859.1"
                     /translation="MAYELPPLPYAKDALEPYIDAQTMEIHHGKHHAAYVANVNKALA
                     GHPDLERKPIEELIRNLGAVPAEIRAPVRNNGGGHANHSMFWKILGPPAGGAPTGKLA
                     DDLKAAFGSFEAFKEKFEAAGVSRFGSGWAWLIVNEGRLEVVSTANQDNPLMGKEVAG
                     VAGKPVLACDVWEHAYYLKYQNRRADYLKAFWSVVNWAAVAKYYDAARA"
     gene            complement(19072..19884)
                     /locus_tag="PKI07_07110"
     CDS             complement(19072..19884)
                     /locus_tag="PKI07_07110"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOC50860.1"
                     /translation="MKSTLLVFLAMGALLLSVPVGMAQNTNDAAAELSALVSKVRAKM
                     EKGVPTASSLAPEIKEFDALLEKHKGEKTDSVARILLMKASLYEEVLKDTAKANSLKE
                     QLKRDFPDSEAARMLKRQEEAEKTSAALAIGARFPDFTEKDLAGNPLSVAQYKGKVVL
                     VDFWATWCGPCVRELPNVLKAYEAHHAKGFEIIGISLDRDKQKLQDFIKDRNMPWPQY
                     FDGQGWQCKLAEKYGVRSIPATYLLDGQGVIIGKNLRGEELERAVAKALAGK"
     gene            complement(19971..21017)
                     /gene="hemH"
                     /locus_tag="PKI07_07115"
     CDS             complement(19971..21017)
                     /gene="hemH"
                     /locus_tag="PKI07_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOC50861.1"
                     /translation="MKKAVLLVNLGSPASPSVADVRRYLRDFLMDGRVLDMPWLARFG
                     IVHGVILPFRPRQSAAAYRRIWTPAGAPLMVTSRNLQAKLQSRVSAPVELAMRYPNPS
                     IPEALRRLVRLGVEAVLVIPLFPHYARSSFETAVARVEEAAAAFTPPLRVQVQPPYFE
                     APEYIAALAGSAREYLQRGYDHLLFSFHGLPERHLRKADPTGRHCLVAANCCTTASPA
                     QATCYRAQCFKTVAAFVRQAGVPEGKYSIAFQSRMGRAPWLKPYTDHELSRLARSGIK
                     RLRVICPAFVADCLETLEEIGLHGRDIFLNAGGVEFERIPCLNEHPRWLDALQQMAQR
                     FIAAPAGTGQGAVR"
     gene            complement(21014..22393)
                     /gene="hemG"
                     /locus_tag="PKI07_07120"
     CDS             complement(21014..22393)
                     /gene="hemG"
                     /locus_tag="PKI07_07120"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HOC50862.1"
                     /translation="MKPVAIIGAGITGLTAGFYLKRKGVPVTLYEAGDRVGGVIQSIR
                     EHGYLAEFGPNTILETSPRVTQLVMDAGLAARRLDPHPRATARYVVRYARPIAMPGSP
                     LGFFTTKLFTARAKLAVLREPFVPRRRDGREESVAEFVLRRLGREFLDHAIDALVAGV
                     YAGDPCRLSVPQAFPKLGQLEARYGSLIRGQIFGARERKRRGEIAKDRAPKFSFDEGL
                     QVLPETLRERLGEAVRLNTAVTRLARTAEGWTLDLRARGQPARAEHSAVVYAGTAFQL
                     AGLEVQAAAPLSLGALAGIRYPPVASVVLGFRREDVAHPCEGFGMLIPRIEGFRILGT
                     IFSSSLFPNRAPAGHLTLTSYIGGERHPELASLPPDKLFALTCEDLRVLLGVRGRPTF
                     QHSVFYPRAIPQYNVGYGRFRELLADGERQAPGLFFAGHYREGISLSDTIVSGGRAAD
                     RAAEFVGVK"
     gene            complement(22390..23757)
                     /gene="hemN"
                     /locus_tag="PKI07_07125"
     CDS             complement(22390..23757)
                     /gene="hemN"
                     /locus_tag="PKI07_07125"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOC50863.1"
                     /translation="MLKVDLELVRKYDVPGPRYTSYPPATHFTYAIAPAEFQARLRAQ
                     NPSARELSLYFHLPFCHSLCWFCGCTSVITTDPAKSAEYVGYLAREMDLLRPLLDPRR
                     KVAQLHFGGGSPTFSPPDEIRRLGEMIHARFALAPDAEAGVEVDPRRLTRDHLTALRE
                     VGFNRGSLGVQDNDPAVQKAVHRHQPFALTRRVVEWMREAGFQSVNVDLIYGLPRQTP
                     QTFARTLDEVLTLSPDRFAVFNYAHVPWLKPAQKILPSGLLPAPEVKLELLKLTIERL
                     TAAGYVYIGMDHFARAEDELAVAQRQRTLQRNFQGYSTRGGADIYAFGMSSISQVEDF
                     YWQNHKELPAYYAALDAGRLPLARGYVMTAEDRIRRTTIMRLMCDLELDYAAMSRRLG
                     LDFPAHFARELDSLDDMAADGLVRKTAQGLAVTDLGRLLIRNIAMRFDAYAATRAGQR
                     FSRTI"
     gene            complement(23751..24785)
                     /gene="hemE"
                     /locus_tag="PKI07_07130"
     CDS             complement(23751..24785)
                     /gene="hemE"
                     /locus_tag="PKI07_07130"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOC50864.1"
                     /translation="MTGRQRFLEACQCRPVDRPPVWLMRQAGRALPEYRALKERYSFL
                     ELVQTPDLATEATLQPIRRFGFDAAILFCDILVVAEGMGQRYEYRDRGGIQMEFLLKS
                     AREVDRLQVGAVTDRLQYVARALAQVKAALGGRAALIGFAGSPWTLANFMLEGGGVRD
                     YTRAKALFYAEPRLFARLFEKLTEAVSAFLQMQIAAGAEVVQIFDSLGGVLSASEFAA
                     ASACWMKRIIRSLPRRVPVIVFSRGTHGNWDELAATGARVLGVDGNVRLADVAARLPA
                     RMAVQGNLDPFLLTTTPALVAAETGRILRALRGRPGHIFNLGHGVPPAAKLENIESLV
                     GTVRSWGGAC"
     gene            25026..25181
                     /locus_tag="PKI07_07135"
     CDS             25026..25181
                     /locus_tag="PKI07_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50865.1"
                     /translation="MQRLDAHASCRITNLDRKQSFTCSGSELAHTGLEATLPDKPGSA
                     LLIYERQ"
     gene            complement(25240..25896)
                     /locus_tag="PKI07_07140"
     CDS             complement(25240..25896)
                     /locus_tag="PKI07_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC50866.1"
                     /translation="MNWSRLRHFAWVDTRARFVAGVPPAGALLDLGSSDGETLGHIAE
                     LRPDLRLFAADLVGAPEKYPAGCQFQRADLERETLPWPGGSMDAITCMQLVEHLRDLN
                     LLMQEAARLLKPGGRIFFETPHPKTLSLPSLQGRAKGMFTLNFYDDPTHVRCVPVATL
                     AACVRGVGLEVVASGISRNWLFAAAHLCYWFLPPSRRKYTARVHWLGWSSFLIARRPA
                     "
BASE COUNT         4693 a         8707 c         8584 g         4439 t
ORIGIN      
        1 gggacaggtt ttgggcgttc ccatgcccaa agcgggcgag gacgcccgcg cgccgtcggg
       61 agtggctaga gcgtattaaa taatactgtg ccgcaaccgg caaaccagtt tcttgcgtct
      121 tggggggatg accaatgcca aagcggctcc gatcgcttcg atcagggcag cctcggttcg
      181 ggcctcaacc tgccgcagga agttcttgat cttgctccac attttctcga tggggttgag
      241 gtccggtgag cacgccggca ggaagaggat ctgagcgccc gttgcagtga ttaagtcgag
      301 ggtcggcgcg cgggcgtccc gcccgcttaa atcgttcgca ctcccacgca aaggggcagg
      361 ttttgggcgt tcccatgccc aaagcgggcg aggacgcccg cgcgccgccg ggaggggcta
      421 gtccgccgtc agcggcaggc tttggctgtg gctgttgaat tccggcgcat agaggcattg
      481 gatttgcgcc gggccggtct ggtattggcc gcgaagctgg acgcgggttg aatactcgaa
      541 cacgtaggtt cccttgggga ggtagtcaat gaagaagtgg ctcgcggtgt cgcgcgtgct
      601 ttcgtagtaa gccaggccgt cctggaaccg gtagcgcgag aagacgttca ccggctcagt
      661 gccgctgccg cgcaggtcct tgagatggac gtactccata tcgcggtcca cgcgcaattc
      721 gaggcgcacg accaactcgt cgcccacatg gaccgggccc cgggccggct ccagcaccgg
      781 cccccgggag gtgttgaccc gggtgtagag cgccttgttc agcttgagcg gcgtattggt
      841 gctggcggtg atttggtcca gttcctccag gtattgcaag tgcacgctgc cccaggcgac
      901 gccggcgtcg gtcttccgga cgatgatctc ccccagcttc ggcttaattt gatccggcgc
      961 aaagcggtat tcgtagaagc cggtgccggg ctcaaccgcc ggtgccggca ggtccttgcc
     1021 gcgcccgggc gcgaccgagc ccgggctcgt ggagccgccc gccggcccgg gaaccggcgt
     1081 cacatccacg ccgcccaggg acatctggac gagggcatcg cgagaaagga ggtccgggcc
     1141 gcgcagcagc agcgcgtaca cggcatccgc ggtggctttc gtcgtcttcc aatcctgcgt
     1201 ctgcttctgt ttgagcagcc agacccggca ttcctccacc gtcggcgggt cggtcaggat
     1261 ttcgtcaaag gcctcaatca tcagggcctg ggtctcgagg ggcgcgcgat accaccacca
     1321 gctccgctcg gcatcgcgcc agaaccggcc cagctcctca tcccgcacgc tgcgttcctt
     1381 gagcgattgc agaatgtcgc gggccgtgga agcgttggcg gcgtcaaggt cgccaaagcg
     1441 atggagggcg agagccaggt ggccctgggt ctggcggcag tcggtctgga gccagaactt
     1501 ccgggactgc ttgaggaaga agtcaatcac cggccggtgt gggggcgtga tcggcaggtc
     1561 tttgcgaaag aagctgcggc cgtagagata cagcgcgtcc gtggcgctgg ggacatgctt
     1621 ttccggctcc ggccccttct ggatgcgcgc atagcgttcc gccatcccgg catccagccg
     1681 gcgcagcgcg cggaggatgg ggggcgcgtc caggctcacg cccaggtgcc ggaggcggcc
     1741 aaagccggtg gtgatataga gcgtgatgta atcattgccg cgcccgcccg ggaaccacgg
     1801 ccaggcgccg tcgtccagtt gctgcccggc cagcttctgc aaggcggctt cctgttcgta
     1861 ggcgaggcgg ttgtcatcga acaggatgcc gagattgcgg cgggcctggc tctcctgatt
     1921 ggcctgccgc agccaggggg tttcttcgag catcaccgcc tggaggtccg ggttccgctg
     1981 gagggggctt tcgagcgcgg gcgtgtcctt ccagagatcg aagaggcggc ggattttggg
     2041 attggaattg gcgatgaagc gcgccagggc attggcgtac aggcggttga aagtctgctc
     2101 ggtgcattcg tagggggatt ccatcaggta aggcagcgcc agcaccgcat accaggccgg
     2161 ctgggacacc atctgcgcca ccaggctctg gttctggcgc gtcggcgcgg cgccggattc
     2221 aagcaaccgg gtgaacgtga acgagttggt ggccgggccg cgaatcggca gcggcaatga
     2281 ttcggtgacc aggacccggc gcgcgagcac cggcagatag ccctcttcgc cgtccgagac
     2341 gcgggccgcc gcgccgacgg cccggtaggt gagaaacccg cacccatccg gcacgtggat
     2401 gcgccaggca taactgcgcg attcccgggc cgggaggtcg aagtcccgct cgggcttgag
     2461 gttgcccagc agcgcgtcgg ccgggtcgtt attgagcgcg tggttgaacg tcagccgcac
     2521 cgtgccgcgc tggggttggt cggtcgggtt cgagaccttg accgtgaatt cgacggtgtc
     2581 gccctcgcgc aggaagcggg gcggattggg ctgcaccata aggtctttgg ccgacacggc
     2641 gtggtcttcg agcgcgccgc tccgcagcgc gcgatcatgc gcgaaaccga ggaaccgcca
     2701 tttcgtcagc gcctcgggga gggtaaaagt catccgcacc acgccattgg aatcagtggt
     2761 cagttgcggg aaaaagaagg cggtttcatt gaggttcttg cgggcggtga cctggctgag
     2821 ttggagcggc ggcgccggcg cgtctgattc ccgggcgcct tcgccgacct ggtcgagcgc
     2881 gttccggacg gcggccgccg gcttggaaaa agctaactcc gatcccatgc ctgcgaccat
     2941 ggcagcaggc accgacgcct tcatttcaat ggcggctccc gaacgggaag cgcggcccga
     3001 attcttgctc cgggcaaaat actggtagcc ccgaaggttc ccactcagtt ccggcggaaa
     3061 cgcgcgatag gaaatctcca ccggctcccc aggcaaattc caacgcccta aaaccgggga
     3121 gaatcgcata aaggcattgg cgaattgcga tcgcagggta gtgtactcgc tccggaagaa
     3181 agaacccgag aacggcttcg gccaggccag cggagcgaac gcgtccagcg acgcatcata
     3241 gagcgtggcc accaactcgg cggcggcgcc gccgctttgg ccctgggggg ttgggccgcg
     3301 aacctccagc gtccatgttt ccttgccccc cggctggagc ttggaaacga agcgctccca
     3361 tttcaagtcg aggtctttgt tttcccaagg cacgcggatc tcgcgcgatt ccaggtaggc
     3421 gcgattctcg tgaatgaagg tgacgtggac aaagcagccg ccgcgcatgg cttcggtcag
     3481 ggccagccga atctgctgcc gggtgacgcc ggacggagtc caatagcgct ggatcatccg
     3541 ctgacgatgc tcgatttcaa tgaaggcccg gccggttttg tagcccgtcc cccacacggc
     3601 catgaattcc tggccgggct gcagttccca attcggcgcg gccagcagat gaggcacctt
     3661 gaggttcagc cggctccccc cagccggcag gacccgcacg ggaagccggc cggtgaccgg
     3721 ctggccgaac tggtcctgcg tctcgatgac cgcgcaatag gcgccggcgg cgagctgaaa
     3781 ggccagccgg gctgtgccgt tggtgcccgt agcaaaactt ctggacgcca ccatccggcc
     3841 caggggccag tggctgggat cggacaagtc cagcgcgggc gagtcagcat cttcggccgc
     3901 cccgaagggc cctcggggtt cggggctgcg cagcggcgga cggtggacct ttgccggcgc
     3961 ccgcagcgcg taaaccttga cctcgccggt ggcggcttgc ggttcgccat cgagcatccg
     4021 ggtctccagt ttcaattcca cgggttgggc atccgtctgc cagtcgtcgg cgctgatccg
     4081 ggcttccagg gcggcgtagc ccacctgcag atggcggccg gcggagcgcg tttcgcccgc
     4141 gccgtcggtc acgtccgcat gaacctggaa cacgaaaacc ggctcatcct tcttcggcac
     4201 cttgggatcg ggccgggcgt cgaactcaat cttgaatgag ccatccgtgg ccgtccgggt
     4261 gatgccgtgg gctatctcct ggcccgggcg ggacggcaag cccccccacc cccatcccca
     4321 ccaccacggc atccgcactt cgcgcacgac gcggtatttg acctgcgcgc catccacggc
     4381 cgcgccggag taattcatgg catggcccac cagaaccacc gtctcgttga gtttcaccgc
     4441 cgactgcggg gcatccagca ccacctcgaa ccggggccgt ttgtactctt caacgtggaa
     4501 cctgccctgg cccggggcgc gcccgctggc cttgagggac attctcccca taaggcgatc
     4561 gcgcggaacc gtaaagctgc cggcgaagga gccataatca ttggcgcgat gctgccggcg
     4621 cgccacctct ttaccattgg cgtcttcgaa tatcaccgtc aacggttcgt ccttgagcac
     4681 cttgtaattg tcttcagccc ggtccaccca gagacagatg cctttgtatt gaacagtctg
     4741 ccccggccgg tagagcgtgc ggtcagtgaa gaagaccgtc ttcgcgtacg gcgacggagg
     4801 tgacgcttcc gaatcatcat agtaccacaa attctccgca gtggccacct cttgaccacg
     4861 atggcgcgcc cggaaccagt agaactgttc cgacaccggc tccagggaaa agcaaccgtt
     4921 ctggtcggtc gtcaggatgg ggtcggtaat acgattgccc tgcaggtctc gatgccacac
     4981 cgacacctta gcgcccgcga ccggttcgcc ggaatcggct tgcagcacaa agccttcaat
     5041 ccggccggcg cgcacccgcg tcaccagggc caggcggctc acccaaacct gcgccaggga
     5101 gacgacattg ttggtctcgc caaagtccgg gcgatggctg gcggcgatga aataaaagcc
     5161 cggcttgagg cggtcgggcg cggggacggc caaggtgtgc tcgtggtagt cggcggtggg
     5221 cggcagtttt accgaccatt ccagcgcggg ggcgcgggac aggatttccc ggtgttcctt
     5281 ttcgcccact aaccggtaat gcggctccgc cgaaagcgcc tcccaatcat acgggatagc
     5341 gcggaagcag accgtttcaa cattgcggta gtgcacggtg atttccggcc acggcgcgtt
     5401 ccacacgcgt tcggtggtta tgcgggccga cggagcctcg atttcggcga cccggttgcg
     5461 gcaccgcttg ccgccgggcg attgcgggaa cgcccgcgcc ccgcgcaacg ccagttcgcg
     5521 ggccgcgacg aaatcatccc gttgctggag cagtcgcgct gcgcaatcga gggccagggc
     5581 ggagaggtcg gaatccgcgt gcgtgtcaat gaaacgctcc agcgcggcct ggtagcgggc
     5641 gtccttgttt tcgccgaagg caacattcca gccccaggtg aggcgttcga gatccgcgtg
     5701 ggcgaaggcc gggttcgagg catccccgcg gtgaaaatca agcagggcct ggtaaagctg
     5761 aatcgcccgc agttccggct gggaagcctc cgcggcgccg gcctgccagg ccagaaactt
     5821 ctccgccgga tcgaagatgg ggctgtcggc ggacacttca aaagcgtctt cggggcgggc
     5881 ggcggcctgc tcgccggagg catagaaggc aagcgcctca tgggcgatga agtcgtagag
     5941 ggtgggacgg taacgatcgg gcatggaacc tttgggcaaa aggtcgtccc aggcgctaat
     6001 gggggtcgcc ttcaggagcg ggccggcacg cagcgccttc tggaatagct ggtcaatgcg
     6061 ctcaaagagc cgcggcaggt cccatgtggt gaagtcgttg ccgggcggaa ccccggtcgt
     6121 ggtgcgctgc atgaagcgcc agcggttctg ctggaagtag tgccaatacc attcggccag
     6181 caacgtctcc agcagcggta ccatctccga aggcgcttgc gcgatctccg cctccaggcg
     6241 cgtgattttc tcctcgggct ggttgccctg aagattgccc tccaaagcga tcttttggcc
     6301 gatggccttc acggcttcgg cgtaggcctt gtccttcaag gccgccgcga tgatgggctc
     6361 gaggttggtg atggcggtcc ggggccgccc ctgcgtgatt gccgcctcga ccgcccgcca
     6421 ttgcgagtcg cgcggcccgg cctgaagcgg cagactcatg agcaacaggg ccgccgtgcc
     6481 ggccgccagg gtgaaaaaag caggccgccg aagctgcgcg gaaaaagccc gggcacggcc
     6541 cgggggcgga ccaaatggga gtctatgcat acaattcctg ttatttcctg cagccgagcc
     6601 acgtcgcccg ctgttgccaa tagaatagac cttcccccgc cagccggcaa gccccttagc
     6661 tttccgcccg gtagcgatga taccgccccc ggccggggct gccgggccgc ctaattttcc
     6721 gccgaatgca atcccccggg gccggcgcgg aaggcgatgt tcatccgctc cgggtcgcca
     6781 gggatgggat gggcgcaatt ccaatagcag gcgccgcagt gaacgcattt ctcccggtcg
     6841 aacgacggca cgccgcccgc gccgggcgcg atggcctgcc ccgagcacat ctcgatgcaa
     6901 atccgggtgc cgcagcgttc gcagagctcg ggatagagga aaagcacatg gtcggcgtag
     6961 ccgggcggag cctgcacctt gccgcccagc aacagggcgt cctggtgaga gacgaggagt
     7021 tggccatcgt aggggatggc cggccagccg cagcgctcca tcagcgcgtg atgcagcgag
     7081 gtcccctggg tggcgcattc cttgcggagc cgctggattt cggcggcggg aatccggccc
     7141 cgataatact cttccagcga ggggatgcgc tgataggggc ggagcggctc gccgcccagg
     7201 ttgagccggc cgcaggtcaa accagccagc gccatgccga tcattccacg caggacgccc
     7261 ttttggaagc catcgcgcgc cttcccggca atgcgggcct cggcctccac ccagcttgcg
     7321 cgccgccgcc gcacgtaggc cgcctccaga ttctgccggg taaacggctt cccggccttg
     7381 agcaattcga gcacgccctc cgccagttgc accccgctga cccaggcttc gtccaggccc
     7441 gacccggtga gcacattggt cgtcccgctg ccctcgccga tgcgggcata gccgtcgccc
     7501 acgagatgcg gctcgccgcg gcgcccggat tcgccgaggg tcttggcgcc ccaggaacgg
     7561 agtttgccgc ccttgagata ccgccataaa tacgggtgca gcatccaatg ctggagatag
     7621 cggtaggccg tgcgcgccgg gctgtcgaac cacgaaggca cgaaaatgcc cagcgaggca
     7681 acgcgatcgg ggtggacgta gaagaagccg aatatctccg gctcgggaaa cccaaacgta
     7741 tgcagcacgg cgcccggctg gaggggcgta tcggcgggca ggtccaccac aaacttcatg
     7801 ccgacggccc agtcgcggat atgatggccc gcgggaagcc caaaccgggc gtcgagctgc
     7861 aacccgaccg ggccgacggg gccatcgcca accaccgtca gcgcggcgtg gatgtccatt
     7921 cccggcatga aaccatcgcc gggccggccc tgcttgtcca cgccctggtc cagcagccgg
     7981 acgccctcaa ccttctggcc ggcaatgagc gcttcggcca ccggggtgcc gggccagacc
     8041 tggaccgtgc cggagctttg cacctgcgcc ccaatccatt gcaggaactg gcccatcgaa
     8101 agcaccagtc cgccggtctt atgcagaaaa gcgggcgccc acggcagctc gaacgcctca
     8161 tgccgaaccg gcagcagccc gcgaaaagcc cggagacagg cgtccgccgc gcgcagcggc
     8221 ccggatcggc ggctgacgcc gaggggatcc agcagataaa ccaccctctc ctccgtgacc
     8281 ggggcggcca tgggaatctc ccgcgggttg agctggggca acgacgcgcg caacccccgc
     8341 gcccgcgtca ccactcccga cacgccaaag ctgaggtcat cggcccgctc gtagcacaac
     8401 acctggggcg gcgcgccggg caggacgcgg ctctcgacgg ccggcgtgcc atcggggttg
     8461 aggagctgcc gggaaagcgt ggtgagaaac cccgccgtgg ccggcccgaa gccgacgcag
     8521 acaatatccg cgtccatgcg ttggcggcca acaggggaag caggctgggc aagttcgctg
     8581 gtcatctgga accgctaatc ctgtgctctc gctattgcct atcgggcggc gccggaattt
     8641 tctgcctgat ttcaaaacca gcacgctcca actcaaggtt ctgcgtttca ctggcgcgca
     8701 tcaggaagtc gaacccatcg ttgccgcagc gggcgcgcaa gtggttcatc atgcggaggg
     8761 ccttctcaaa tgaatcatac cggccctgta tgtagaaggg cttgccgttt ctgccaaagt
     8821 tgaagctggt cgtggactcc gcggcgtttg tgccgccaaa gacccggcag gccgctttgt
     8881 aattaggatg cggtgccagg ccgaagcccc gtgcgtaggc cacggcgccc tcgaccagct
     8941 ttcgcgcgga gggtgggtct attgccttcc tgtcggcggc cggcagcacg tcctccagca
     9001 gttccatgtc ataccggccc tcgctcactc gtgcatagaa ggcgtctttg actccctggc
     9061 aataaacgtc caccaggaac acgccaagct ctgcgtctcc gccccgaaag cgggcaaaca
     9121 cgacgtggcc gaggccaaat tcaaagaggt tcccagtgac gaaagcttcg gtggcgcgtt
     9181 tcatgggtgt gcggaaatca ctaccccggg taatcgaggg cctcgggaat catcaccttg
     9241 gtcagcgcct ccgccgcgcg atccttggcc aggcgggagc cggtcaggca gccatcgagt
     9301 ttggcgcgca ggcgggtgaa ggtttcgaga ccatccgccc gggggcacgg accggctttg
     9361 cgcggatggg cttcgccggc ctcggtgaca tcggcgcagg cacgcgccga gctgtcaatg
     9421 ccgggaatga tgccctcgag cgactccagt tccgcggccg aatagcaggc ggcgcggctc
     9481 gccgaatccc aggccgggtg ccgattaaac ccatagacca ggtcggcgca aatgcgccca
     9541 acctcgccgg cggcgcgggc gctctgcacc tggcagagat cggcgaaaaa gttcacgaac
     9601 cccggcaagc cgtccgccag cgccgggttg gccgggcctt tttcctccag ctcccggaca
     9661 tccagaataa actggcgcgc cgccagcagc cagcacagcg cgtcggccag ggggaaggtc
     9721 acgccctggc ggctcttgtg ataaagcttc gcgccgtcgg cgtccgtggc tttctgcagg
     9781 tattccagcg tccagagcca gagcttcatg gccgtggcca gcgcgcaggc gcccgtgccg
     9841 gggcgcgcgg cggcgaggcg ggccagttcg gcgatccaca gccggaactg cgcgaggaac
     9901 aactcgttgg tcatggtaag cgagagctgg aggcgctgga cggcttccgg cccctcgtag
     9961 gtggcctcaa gctgggcgtc catccatttc tggcccagga atcccgggca atcttcggtg
    10021 acgccatacc cgcccatcag gctgaccgcc tcgcgcatca tgttggcgcc atgaccagtg
    10081 ttccacagct tgcaggcggg gcaaagcaca ttggcctgag aatccagcag ggcaaactgc
    10141 accagcggat ccgccgccag cgcttcccgg cgcgactcgt cgcgcgcgtc cgccggccgg
    10201 gcgtcaagct gcagcaattc ctgggcttcc tgctggcttt gggcgagagc cttgaaggcg
    10261 gcgcgcccgg tcagctttcg ggcggcaaga atctcatcct tcttcttctc caggggatca
    10321 agggcgtcaa agagccgcgc cgcggcaaag cccagggacg cgctggcctc gccggtggcc
    10381 caaacctcga ccaggcgctg gagcacgtct tccttttgct gcaaccccag ttcgtagcgc
    10441 ggggtgccgg gcgcgccttg cccgccccgg aaccgtcgcc gggcatacat aatgaccggc
    10501 tcgatggccg acaagagctt ggccgaagtc atcagcccca cggtgacgcg cgtgcggcgg
    10561 aagaccgcct caatcatctc gccgtgcgaa taccgcggaa taataacgcc gtctttgacc
    10621 gtgtagccgc cgataatacg gctggcgggc acgcgcaagc tcaacaccgg gtcgcgcgtc
    10681 gaggaaagct gatgcaccag cttccgggtg ggcgtgccgc gatcccaggc gcccgggtcg
    10741 gtctcctcca gaatgaccat gcaagagcct ttgagccgcg ggtcatcgga ctcgaccgcg
    10801 gcggtgacga cgttggcaaa gcccatgttg gtgatgaagc ggccccgctt ttcgacctgc
    10861 agcacgggct cctggccctc gttccattcc gcaacgcgga cccgcccggc cagcatcccc
    10921 gtttcgaccc ccacaaaagg aatcggctcg gtgagcgcaa aggcggcacg gacctgcgcg
    10981 cggctttcgc ccggcttggg cggcgcggcc agggccaggt aatggttgcg ctgggccggc
    11041 gtgccgcgct cgtgaatggg agccaggccc aggttgccgg ccagactgca cgtggccgcc
    11101 ccggcatcca cccaggacaa ttcaaacgcg atcagggcca gtgccaggtt cttggggccc
    11161 tcgataaacc cgccgtgctg cggatccagg aaggccgcgg tgatgccgct ctcatcaaac
    11221 gctgccagga ggctggcttt ctcgggcgtc cactcatggg aattgcgcgc gccctgggcg
    11281 accagccggg cgaccggccc gcgcgccacc gtgcggctgg attgcaccag catggcgagg
    11341 tcgtagcgat cggcaaagcg ccacatgatt tggcgcagat cgtctccagg caatgtttgc
    11401 agcgccgggg gcgccggggt atcagcggca acagacatag atgctcggtc tcagttgaac
    11461 aaacttaaat attcagccgc acgcgctctg gccggagcca ttccggcacg cggtgcgcga
    11521 gtcgaattaa gctatctgcc gccgcagcgc aagccaaaat gcccggccgc ccaggcccgc
    11581 ggcgccgctc aatttccacg ggcgcccggg aaccgactgt ggagaaagtg taaaacctcc
    11641 aaaaccgcaa agaaccctgg cgagccgtca tccgccccgg aggcgtgctg ctgccacttg
    11701 gccgccgtcc aagccagaaa ccctgcagga gttcccgccg caagcccagg atgagcgaag
    11761 aacgagcgtc atccagggaa accagcaccc cccaaatcaa ttcccactcc cctgccactg
    11821 aggtatggaa ccccgggagg agaggaagcc ggcgccctgt ttcgcacgcc cttgccccgg
    11881 ccaattcgag cctgcctccc tcgcctccgg gcatcgcgac cgccaaagca gcccgcatgg
    11941 gcagcagtca ggatctcctt aaaaatcagc accttctcag cggccgctac catccctacc
    12001 cgccgtgtac aagggatgta caagggatgc tcaccggatg cgccagctaa aatcccatgt
    12061 aatcccgttg agcatccctt gtacacccct tgtacacggc ttattagtgg ctatgaatat
    12121 gcctggaatg actgggatta gatgaaaagt ggtgcttgga atcgctttcg aggtcgcaaa
    12181 cgggaaactg tccaataatt ggacagtttg cggcggaatg gtagggcttg ccccgccacc
    12241 ttattcgggc ggggatattg ccaggatgag gttaagaggc atgggctttc cgggtatccg
    12301 gggtcctggc cccttccccg ggcgtcacgg ctgggagtgc gggatttccg tttccggtct
    12361 tccgccccgg cgaaagattc tttcgcttga tcccattaaa ccacccagcc gtgcgccgac
    12421 gggttccccg ttgcttgcgc gcgggacgca gcgcgcccaa agttcgtcac cgccttaaac
    12481 tggcgctacg caaccctgcg aaaaggctgg ggacagattt agcacacacc gcccggggct
    12541 gtctgtcggc ggatttgctg atttttttgt aaggaaaaac cttacacgcg gaagggcgtc
    12601 ttaacggcgc cgggtccagg aaatggcgtg ctggagcagt tggctggagg tttggagatt
    12661 aagtttctcc cggatgcggc ggcgataggt atccacggtc ttggtggcga tgcggagccg
    12721 gcgggcgatt tccttgacga ccagtccgcg gccaagcagt tcgaacacct gcaactcgcg
    12781 gtccgccagc atttcggcgg gcgtggcggc gcccgggacg gagcggtcgg ccaaccggtc
    12841 aataatccgg gcgctgacgt tttcgttgag gtacacgcgg ccgctcaaca cgcggcggat
    12901 ggcggagagg atgtcgcgcg tggcttcctg cttggtgaca tagccccgcg ccccggcgcg
    12961 aatgacgcgc tcggcgtaca gggactcctc gtacatggaa acgacgagca tgagcacatt
    13021 gggccagtga aggcggatat ccttgatcag ctccagcccg tccgagtcct tgagcgccag
    13081 atcaatgatg gccagttccg gccggtgggc agccatcgcc gccagggcgc ccgcgcggtc
    13141 ctgggcttcg ccacacacca tgagatccgg ttcctggttg atgatttcgg ccagccgttc
    13201 gcgcaccagg ggatggtcat ccaccagcag aatgcgcgcg cgccgggccg ccggacgggc
    13261 cgtggaggat ttgcgtctca tgcgggggat tgtccgcccg agcccacggg cgcgcaacaa
    13321 gaaacgagcg tgccgcccag ccggctcgcg ccgatccgga aggtgccgcc gatggcgcgg
    13381 gcgcggtaat ccatgatatg cagccccatg ccgccgcccg cgccgggttg gggggcggag
    13441 aggccccgcc cgtcatcctc gatgcggagc tcaaggcggc cggcgtggag gtcgaggtgg
    13501 atgctgatgc ggcgggcctg gctgtgcttg acggcattgg tgacggcttc ctgggcgatt
    13561 cggtaaaggt gcgtggccag gacggcatcc gccgcaatca cggacttcgg cggcgagaag
    13621 tggcactgga ttttgaagcg gcggccggtg gtgctcgcca aatcttccag ggcgtgcggc
    13681 agcccttcgg ttccgagctg gacggggaac aggccgcgcg ataattggcg cgcttcggta
    13741 atggcgtggt cgaggtaaag ggcgatgcgg cgcgctttgc tggccgggcc gcgatgtttg
    13801 ccggccaggg cgcggtgcag tgaattggca tcaaacgcca ggctcaccag gtgctggcaa
    13861 agcccgtcgt ggatgtcctg gccgatgcgg gcctgttggc ggtcgctgat ctccaggatt
    13921 tgcccttcca ggcgctggcg atcggtttcc gccttgatgc gcgcaatggc gcccgccgcc
    13981 tgggcggcga tggcttcgat cacgatctga gtttgccgcg ggatttcatc ggccactttc
    14041 gaggccagcg tcagtgcccc aatgatccgg ccgttgtggc tcaacgggat aaaggcggaa
    14101 gcccgcaagc cctcgctccg ccgcggcgcg gtatgcgaaa tcggcaggtc ctggtaaagg
    14161 cagaaaacgg gctgtccttc gcgcagcaat tgcatctggg ggctgttggg cgtccagctt
    14221 gagacggctt tgatgaacgc cggagaaccg ccccggtgca cggccagggt catttcgccg
    14281 ctggcctcgt tgagcaggta aacgccgccg ctgtccaccg cgcccagccg caggatgata
    14341 tcgagcaggt gtctcagggc gacatccaga tcgctggtca agctgaagcc ggcccccaga
    14401 tcgcgttgcg cctggagcag gatatccgcc tgctttcgtt tggagatgtc ccggcaaagc
    14461 cccatcatcg ccgttgcagc cccccgcgca tcgcgcaagg gcacaaagcg aacttcgagc
    14521 cattgatcgc ccgcgggaaa cggcgccgcc tcgatggcgc gcgcgggctg gcccgtccca
    14581 aacacgtcct gcgccagggc ttcgaggcag ggcccggttt ctgatgggaa gagcgccgcg
    14641 gtcgtctccg cgagaaggcg ctcgaccggg cggcgccaga tggccgccgc cgccgggttg
    14701 gcatattgca ccttgagatc ccggtcgaag atgaggatgg catcgggaga cgattcggcc
    14761 agcgcccggt agcgctcctc gctggcgctg agcgcctcct ggatgcgttt ctgctgcgtg
    14821 atgtcttcga gcacgccgtc gagccaatcc acttcgcccc gggcattgcg gtggaccgtg
    14881 acgttgaccg aggcgcagat gggggtgccg tcccttttcc ggagcagcag ctcgtagttg
    14941 cgcacgcttc ccgcgcgcag cagctcggcc agcaacgccg cccgctggcc gggatcctgg
    15001 tacgtatcca cgatgcggac gttccggatc tcgtcggcgg aatcataccc gaggatgcgg
    15061 gccagggccg gattgacttg cagcaggagc ccctcggcgt cgggcgtgtt tcggtaaatg
    15121 cccacgttga ggttgttgac cagcgagcgg tagcgttcct cgctttggcg gagggcggtg
    15181 ttggccgcgt gcagttcggc cgttcgctcc cggacgcgct gttcgagttg ggcgtgcgcc
    15241 tggttcagcg cctggttggc ggcctgcagc gccgtggtgc gctcttgcac gcgccgttcc
    15301 aactggtcat gcgcctcgcg cagcgcctgc tcggcgcgaa tgcgctccaa cgcaccgccg
    15361 cacaggtcgg ccagggcttc gagcgtgcgc aagtcttccg gcgtaaaggt gttgaaggcg
    15421 tagctttgga tcgaaaggat gccgatggtt tgatccttgc gacgcaaggg ggcgtacatc
    15481 agggaggcgg agcggcgctg cgcatcaccg aaggccacca ggtccgcgga ttgcagttcg
    15541 gcgggggtgc gcaggatcaa ctccgcgccg ttctcgatga tttggcgcat tcggggcagc
    15601 ggcgagctgt ccgtgccagc cgaagaaacc tcgcggcgct ggccgtccag ggtgtcaatc
    15661 aacagcacgg actccaggca atcccgctcg gcggaataca gttgcagcac ggcggcgtcc
    15721 cattgccaga gctggtccgc ggcggtgacc atcgcccggc cggccgccag gggactgtcc
    15781 acctcgctga ggtgggtcgc cagcgtcagg aatgcctgcc ggatcaggct ggcccgtttg
    15841 gtttcggtga tgtcccggat cacggccagg gagcctggct gcccgtccgg cagcatcagg
    15901 ggagttgcgt gaagcgccat caatcggcgg tcaccgtcct ggcggaggcc ccacagttcg
    15961 ctctggagcg agcggccggc gcgcagcgtg gccaggtgcg cttcgacttt gggttgttgc
    16021 tccagcggcg cgaagccccg cacgttgcgc cccagcaact cctcccgcgc atagccaaac
    16081 gcctggcaaa gagcctggtt ggcatcgaga atcgttccgc cggcgtctcc cgccacgatg
    16141 ccgtcggggc tcaactcgaa gagcgtgcgg tagagttcct ctttccgggc cagggcggtt
    16201 tccgactgtt tgcgctcggt gatgtccagc gcgaagatgg ccacacggct cacattgccc
    16261 acggcatcga gaacgggatt gaaaaagata atatagtgcc gtccctggtg ctcctcttca
    16321 aatcgcgccg gcttgcgggt ttgcaggatc cggtcaaagg agtttttcca gtcgggcagc
    16381 attcccggcg gggtgatatc aaagatgttc cggcccggga gttccggggg ggagcgatcc
    16441 atcccgacgc tcgccgccgg gttggccagc aggatgacgc cctcgcgatc caacagcagc
    16501 gccggctgcg gcaccgcgtt gagaaagacc cgcaggcttt cctcactttg cttcaacgcc
    16561 agttgcgccc ggttgcgctc ggtgatgtcc tggccgacga gcaccatgaa gaagcggccg
    16621 tcgagggtga acccgctgct gcggcattcg atctcgaaca ccgagccatc cttgcgccgc
    16681 acgaggcgtt catcccggag aaacccccgg gttttgactt gatcgaggaa gcaagcgaac
    16741 atccgctgga actcctccgg gctgcgggcc gacagggttt ccagcgggcg cccgatcagt
    16801 tcgccacgcg catagcccac ctcggcatcc agggcggggt tggagagcag gattgttccc
    16861 tgctcatcca ccatcaatac ggcctccccc atgctctcca gcacgcgcgc ctgcatttgg
    16921 agctgttcct gggtgcgccg gctctcgctc acatcctgtt ggagggcgac gtagagcgtt
    16981 cggcccgcca cttccacccg gctcagaaag gcgtcgcaat aaacgaggcg gccgcttttg
    17041 tgccggttcg tgaatttccc gtgccaggac ccggtggtcc gcagcgcggc caggacttcc
    17101 tgcataacgg ccgccttctc cggctccggc agcacgctaa tactgtcggc gggctgcccg
    17161 agcatctcct ccagttggta gccccacatg cgctccgccg cggggttcat ttgcacgatt
    17221 cggccctgtt catccgtgac caccacgccc tccgtcatgc cctcgagaat ggccgcccgc
    17281 gtttggagtt cttcgcgcgc ccgtttccga tcggtgatat cgtccgcaag cccggcaatg
    17341 cgatagacct cgccctgggc attgcgcacc gggaaacccc ggtcgcgaat ccagcggatc
    17401 gtcccgtccg gccggatgat gcggtattcg atatcgtagc cgccggtgga ctggtcagca
    17461 tgggcgctgc gggacacggc ttcgcgatcg gccggatgaa ccgccgccag ccaggacagg
    17521 ggctcgcggt aaagctccgc gcacgtccgg ccccacaggc gctcatagcc ggggctgatg
    17581 taaaccatct ggcgcttgga gacatccgac agccagaagg cttcggtgat attgttgatg
    17641 acctgctcaa aacgcgccag ggtttcggcc agccgggccg ccgtggactg gtgttcggcc
    17701 acttcggtct tccattgcgc cgtccgttcc tcgaccagtt ggctcaaatg acgcgccaac
    17761 cgggcgacct ccgccgccag gcagccgacc acgctgaacg ccaggaaacg cgtggcggca
    17821 ttccacagca gcagccagcc gggctgcgga atctcgcgat gccaatacca ctgcacggtg
    17881 agcatggaaa gcgcggccac cgctgcgacc agccccgcgg gccccttgcc cgccccccag
    17941 ccggtgaaaa tcaccgcgaa caggtagaac agggtcaggc tcgcggcgtc cggggtgaaa
    18001 cagtcgagcg cgccaagcgc cagcacccaa cccaaaccca tggccgtaat cgtgaaggac
    18061 ggcaatcgct ggagcctggc ctccagcctt tgaatcagga ccttcataca cgttgatcct
    18121 accatcccgg ccgacgtctg ttaagccgga attcgcgccc tccgcctgcc gggatgcggg
    18181 tgcgcctgcg cgccgcttga ctttagcccg atcatggtgt aagcattagc gaagattaca
    18241 atggcattcg tttaatcaac gttaacaaca ataagaaaaa tattatggca tacgaactac
    18301 ctccccttcc atacgccaag gatgcgcttg aaccttacat tgacgcgcag accatggaga
    18361 ttcaccacgg caagcaccac gccgcctatg tggccaatgt caacaaagcc ctcgccggcc
    18421 acccggacct ggagcggaag ccgatcgagg agctcatccg caatctgggc gcggtgccgg
    18481 cggaaattcg cgccccggtg cgcaacaacg gcggcggcca cgccaaccac tccatgttct
    18541 ggaagattct ggggccgccg gccggaggcg ctcccacggg caaactggcc gatgacctta
    18601 aggccgcctt tggcagcttc gaagccttca aagagaagtt tgaggccgcc ggggtgagcc
    18661 gattcggcag cggctgggcc tggttaatcg ttaacgaggg ccggctggaa gtggtttcca
    18721 ccgccaacca ggacaacccc ctgatgggca aagaggtggc cggggttgcc ggcaaaccgg
    18781 tgctggcgtg cgatgtatgg gagcacgcct actatttgaa ataccaaaac cgccgggcgg
    18841 attacctcaa agccttctgg agcgttgtca actgggcggc ggtggccaaa tactacgacg
    18901 cggccagggc gtaaacccgc cgcttcgaag gctcaggaaa cgcgccggga ccggccgggg
    18961 aaacggatgt cgggcgccgg caatctccgg ggcggagggg agcttcccct cccgcgcccc
    19021 cgggcgtggc ttcatgcagg cccggcggag ctttgacgcc ggtggcgcgg tttatttccc
    19081 ggcgagtgcc ttggcgacgg ctcgctcgag ctcctcgccg cgcaggtttt tgccaatgat
    19141 gacaccctgg ccgtcgagca ggtaggttgc cgggatgctc cggacaccgt acttctccgc
    19201 cagtttgcat tgccagccct gcccgtcaaa atactgcggc cagggcatgt tcctgtcctt
    19261 aatgaaatcc tggagcttct gtttatccct atcgaggctg atgccgataa tctcgaagcc
    19321 cttggcgtga tgggcttcgt aggccttgag cacgttaggc aactcgcgga cgcacgggcc
    19381 gcaccaggtt gcccagaaat ccaccaacac cactttacct ttgtattgag cgacggaaag
    19441 cggattgccc gcaagatcct tctcggtgaa gtcggggaac ctggcgccga tcgccagggc
    19501 ggcactcgtt ttctcggctt cttcctgccg cttaagcatc ctggccgctt cggaatcggg
    19561 aaagtcccgc ttgagttgct ccttcaggct attggccttg gcggtatctt tgagaacctc
    19621 ctcgtacagg gaggctttca ttaaaagaat ccgcgccacg ctgtcggtct tttcgccttt
    19681 gtgcttttcg agcagggcgt caaattcctt gatttcgggc gccaggctgg acgcggtggg
    19741 gacgcctttt tccattttgg cccgaacttt ggacaccaat gccgatagct cggcggcggc
    19801 gtcattcgtg ttctgcgcca ttccaaccgg aacggacaac agcaaagccc ccatcgccag
    19861 gaaaaccaga agcgtagatt tcatgggagg gaacgtcgct cataaaacgg cttgccgcaa
    19921 gtgccaatta ctgccgcggg tttggagtgc gagccccaaa ccgcccgcgt ttagcggacg
    19981 gccccctgcc ccgtgcctgc gggcgcggca ataaaccgct gcgccatctg ctgcagcgcg
    20041 tccagccagc ggggatgttc gttcaagcag ggaatccgct caaactccac cccgccggca
    20101 ttgaggaaga tgtcccgccc gtgcaggccg atttcctcca gggtctccag gcaatcggcc
    20161 acgaaagccg ggcagatgac ccgcaaccgc ttaatgccgc tccgcgccag ccgggaaagc
    20221 tcgtgatccg tatagggctt gagccaggga gcgcgcccca tccgggactg gaacgcgatc
    20281 gagtatttgc cctccggcac cccggcttgc cggacgaagg ccgccacggt cttgaagcac
    20341 tgcgcccggt agcaggtggc ctgggcgggg ctggcggtcg tgcaacagtt cgcggccacc
    20401 aggcaatggc gcccggtggg atcggctttg cgaaggtgcc gctccggcaa accgtggaag
    20461 ctgaacagga gatggtcgta gccgcgttgc aaatattccc gcgcgctgcc cgccagcgcg
    20521 gcaatgtact cgggcgcctc aaaatacggc ggctgcacct gcacgcgcag gggcggggtg
    20581 aacgccgccg ccgcttcctc cacgcgcgcg acggcggtct cgaagctcga ccgcgcgtag
    20641 tgtgggaaca agggaatcac cagcaccgcc tccaccccca ggcgcacgag acggcgcagg
    20701 gcctccggaa tggacgggtt cggataccgc atggccagct cgaccggcgc gctgacgcgc
    20761 gactgcagct tcgcctgcag gttgcggctg gtgaccatca gcggcgcgcc ggcgggcgtc
    20821 caaatccggc ggtaggccgc ggcggattgc cgcgggcgaa agggcaggat gacgccatgc
    20881 acaatcccga agcgcgccag ccagggcata tcaagcaccc ggccgtccat aaggaaatcg
    20941 cgcaggtagc gccgcacatc ggccacggaa ggggaggccg gcgagccgag attaaccagc
    21001 aggacggcct ttttcatttt acccccacga actccgccgc ccggtccgcc gcccggccgc
    21061 cggagacgat ggtatcgctc aaggaaatac cctcgcggta atggccggcg aaaaacaggc
    21121 cgggtgcctg ccgctcccca tcggccagca actcacgaaa acggccgtag ccaacattat
    21181 attgcgggat ggctcgcgga taaaacacgc tgtgctggaa ggtgggccgg ccgcgcaccc
    21241 cgagcaagac gcgcaggtct tcgcacgtga gcgcaaacag cttgtccggc ggcagcgaag
    21301 ccagttccgg gtggcgctcg ccgccaatat aactggtcag cgtcagatgg cccgcggggg
    21361 cgcggttcgg aaagagcgag gaggaaaaaa tcgtgcccag gatccgaaac ccctcgatcc
    21421 ggggaatcaa catgccaaat ccctcgcacg ggtgcgccac gtcctcgcgc cggaagccca
    21481 gcacgacgct ggccaccggc ggatagcgaa tcccggccag cgcgccgagc gacagcggcg
    21541 ccgcggcctg aacttccagc cccgccaact ggaaggccgt gcccgcgtac accaccgcgc
    21601 tatgctcggc gcgcgcaggc tggccgcgcg cgcgcaggtc cagcgtccag ccctccgccg
    21661 tccgggcaag gcgggtgacg gcggtgttca ggcgcaccgc ctcgcccagg cgctcgcgca
    21721 gcgtctccgg cagcacctgc agcccttcgt caaaggagaa cttgggggcg cggtctttgg
    21781 caatctcgcc gcgccgtttg cgttcgcgcg cgccgaagat ttgtccccgg atcagcgagc
    21841 cgtagcgggc ctcgagttgg cccagcttcg gaaacgcctg cggcacggaa agccggcaag
    21901 ggtcgccggc atagaccccc gcgaccagcg catctatggc gtggtccaga aactcccgcc
    21961 ccagccggcg caggacgaac tcggctacgc tttcctccct gccatcgcgg cgccgcggca
    22021 caaacggctc gcgcagcacg gccagtttgg ctcgcgcggt gaacagtttc gtggtgaaga
    22081 agcccagcgg cgagccgggc atcgcgatgg gccgcgcgta gcgcacgacg tagcgcgccg
    22141 tggcgcgggg atgcggatcg agccgccgcg cggccaggcc cgcatccatc accagttggg
    22201 tcacccgggg cgaggtctcc aggatggtgt tgggcccaaa ctcggccagg tagccgtgct
    22261 cgcggatgga ctggatgacc cccccgacgc ggtcgcccgc ttcgtacagc gtgaccggca
    22321 cgcccttgcg tttcaggtag aagccggcgg tcaggcccgt gatgccggcg ccgatgatgg
    22381 cgaccggctt catatggtcc gcgaaaagcg ttgcccggcg cgggtggcgg cgtacgcgtc
    22441 gaagcgcatc gcgatattgc ggatgagcag ccgcccgagg tcggtaacgg ccagcccctg
    22501 cgccgttttc cgcaccagtc cgtcggccgc catatcatcg agcgagtcca gctcgcgggc
    22561 gaaatgggcc gggaaatcca gcccgagccg ccgggacatg gccgcgtagt caagctcgag
    22621 gtcgcacatg aggcgcatga tcgtggtgcg acgaatccgg tcttccgccg tcatgacgta
    22681 gccccgcgcc aggggcagcc tcccggcgtc cagcgcggcg tagtaggccg gcagctcttt
    22741 gtggttctgc caatagaagt cctcgacctg cgagatggac gacatgccaa aggcataaat
    22801 gtccgccccg ccgcgcgtgc tgtagccctg gaaattgcgc tgcagggttc gctgccgctg
    22861 cgccaccgcc aactcatctt cggcgcgcgc gaaatggtcc atgccgatat acacataccc
    22921 cgcggcggta agccgctcga tggtcagctt gagcagctca agcttgactt ccggcgcggg
    22981 caggagtccg gacgggagaa tcttctgcgc cggcttcagc cacggcacat gcgcgtagtt
    23041 aaagacggca aaccggtccg gcgacagcgt gagcacctcg tcgagcgtcc gggcaaacgt
    23101 ctgcggcgtc tggcgcggca ggccgtaaat caggtccacg ttcacggatt gaaaccccgc
    23161 ttcgcgcatc cactcgacaa cccgtcgggt cagcgcgaag ggctggtgcc ggtggacggc
    23221 tttctgcacc gccggatcgt tgtcctgaac ccccagggaa ccgcggttga agcccacctc
    23281 gcgcagggcc gtcaaatggt cgcgcgtgag ccggcgcggg tccacctcca cgcccgcctc
    23341 ggcgtcgggc gccagcgcaa agcgcgcgtg aatcatctcc cccagccgcc ggatttcgtc
    23401 cggcggcgaa aaggtgggcg acccgccgcc gaaatggagc tgcgccacct tgcgccgggg
    23461 gtcgagcagc ggccgcagca aatccatttc ccgcgccaaa tagcccacat actccgcgct
    23521 cttcgccggg tcggtggtaa tcacggaggt gcagccgcag aaccagcaga gcgaatggca
    23581 gaacggcaga tgaaaataga gcgacagctc ccgggcggac gggttttggg cgcgcagccg
    23641 ggcctggaac tccgccggcg cgatcgcgta ggtgaaatgc gtcgcgggcg ggtaggacgt
    23701 gtagcgcggc cccggcacgt cgtatttcct caccaattcc agatctactt tcagcatgcg
    23761 ccgccccagc tccgcaccgt gccgaccagg ctttcaatgt tctcgagttt ggccgccggc
    23821 ggcaccccgt ggcccaggtt aaaaatatgc ccgggccgcc cccgcagcgc gcgcaggatg
    23881 cgcccggtct ccgccgccac cagcgcgggc gtggtggtca gcaaaaacgg atccaggttg
    23941 ccctgcaccg ccatgcgcgc gggcaaccgc gcggccacgt ccgccaggcg cacgttccca
    24001 tccaccccca gcacccgcgc gccggtggcg gccagttcgt cccagttccc atgcgtgccc
    24061 cgggaaaata cgatcaccgg cacccggcgc ggcaacgacc ggataatccg cttcatccag
    24121 cacgccgagg ccgccgcaaa ctcgctggcg gacagcaccc cgcccaggct gtcaaagatt
    24181 tgaaccacct ccgcgcccgc ggcaatttgc atttggagga acgcgctcac cgcttccgtc
    24241 agcttctcga acaagcgcgc aaacagccgc ggctcggcgt aaaagagcgc tttggcccgg
    24301 gtgtagtccc tcaccccgcc gccttcgagc atgaagttgg ccagcgtcca cggcgagcct
    24361 gcaaacccga tcaaggcggc gcgccccccg agggcggctt tgacctgcgc caacgcccgg
    24421 gcaacgtatt gcaaccggtc ggtcaccgcc cccacctgca accggtcaac ctcccgggcc
    24481 gatttcagca aaaactccat ctggatgccg ccccggtcgc gatactcgta gcgctggccc
    24541 attccctcgg ccacgaccag aatgtcgcag aacaggatgg ccgcatcgaa accaaatcgg
    24601 cggatgggct gcaaggtcgc ctcggtggcc aggtccggcg tttggaccag ttcgaggaag
    24661 gaatatctct ccttgagcgc gcggtattcg ggcagggcgc gccccgcctg gcgcatcagc
    24721 cagaccggcg gccggtccac ggggcggcac tggcaagcct ccaggaaccg ttgccggcca
    24781 gtcatgggcg ccggtgccag gcccggcctg gccgccctgg ccctcttgcg taaaggagcg
    24841 cttcgtctca tgcggaatcc tattatactt gcccgggttt ccagccgggt caattactgc
    24901 tttgtacgcc ttcccgaaac gccgcggatg ccgcttcgga actgccggat cggccatccc
    24961 ccggcaaggg cctcgtcgtt gccccgcgcc ggcctgacag cccctgccaa tccgcccggt
    25021 tcaacttgca gagactggac gcgcacgcct cctgccgcat caccaacctc gaccgcaaac
    25081 agtcgttcac atgctccggc agcgaattgg ctcacaccgg cctcgaagcc acgctgcccg
    25141 acaagcccgg ttccgccctg ctgatctacg aacgccagta acccgaaaga acggttcccc
    25201 cggctgacgc gccgaacctc cggcggcggc ggcgacaatc tatgccggcc ggcgcgcgat
    25261 gagaaagctc gaccacccca gccaatgcac ccgggcggtg tacttgcggc ggctgggcgg
    25321 caaaaaccag tagcaaaggt gggcggccgc aaatagccaa ttacgcgaga tgccgctggc
    25381 caccacctcc aggcccacgc cgcgcacgca cgctgccagc gtcgccaccg gaacacaccg
    25441 cacatgcgtg gggtcatcgt aaaagttcag cgtgaacatt cccttcgccc ggccctgcaa
    25501 gctgggcagg gacagggtct tgggatgcgg ggtttcaaag aagattcggc cgcccggctt
    25561 cagcagccgc gccgcctcct gcatcagcaa gttcaaatca cgcaaatgct ccaccagttg
    25621 catgcaggta atggcatcca ttgacccccc gggccagggc agcgtttcgc gctccagatc
    25681 ggcccgttga aactggcacc ctgcgggata cttctccgga gcgcccacca ggtcggcggc
    25741 aaaaaggcgc aaatccggcc gcaactcggc tatatgcccc aaagtctcgc cgtcggacga
    25801 ccccaggtcc aacaaggcgc cagccggagg gacccccgcc acaaaccggg cccgcgtgtc
    25861 aacccaggca aagtgtctca ggcggctcca gttcatgccc cccgcaaacc gcgccgacgc
    25921 caatccagga tggcggcggc cggcctgact gtgattcgtg tgatataaac ggccagcaag
    25981 cgaaagagat tgcaaccgat ggcaccgggt tgccgcaagc taaaactccc gccatccgcc
    26041 cacgacccga actccgcctt ccccgacaga caactccccc tgaatcagac aacctctcct
    26101 gcccgattaa cctttacagc aaagccgccg caaactgtcc aattattgga cacttccact
    26161 gcccttgcca gcccccctgc tccatgcctg agctcgcatt agtcgaaatc ccactcaact
    26221 acactcgttg caagctttta tatggctgcc aacaagccgt gtacaagggg tgtacaaggg
    26281 atgctcatcg ggtgtaccag ctaaaatcct atggactccc ggtgagcatc ccttgtacac
    26341 cccttgtaca cggcgctttg gcagggggaa aagacggata ttggagggag ttttgctgct
    26401 ggcgtgggga cgccgggccg agg
//
LOCUS       DAOPQG010000047        26186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2387_length_26186_cov_15.879530, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000047 DAOPQG010000000
VERSION     DAOPQG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 26186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26186
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2387_length_26186_cov_15.879530"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..950
                     /locus_tag="PKI07_07145"
     CDS             246..950
                     /locus_tag="PKI07_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOC50867.1"
                     /translation="MKRITITRFILAHVSAVALVAAPSAWADTLAQWTFESSGPSIAG
                     TSTTFGPIAPEVGSGNAYGVHASASTVWSSPVGNGSPESFSANNWSVNDYFEFTIPST
                     VGYKDVSISYDQIGSATGPRDFSFTYSTDGSIFITVGSYQLTSGVSWSGTIGGRPDQL
                     SFLNISALDNWNGTIYFRVVDDSTTSINGGTVAAGGTGRIDNFTVTATMIPEPSALAL
                     VGLGVAGLVAFRRRNG"
     gene            1124..2929
                     /locus_tag="PKI07_07150"
     CDS             1124..2929
                     /locus_tag="PKI07_07150"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOC50868.1"
                     /translation="MTQNPFESSDTVRHAQPLRHVRLARLAQPLALEMGGQLAEVTLA
                     YETYGELNAARDNAVLICHALSGDSHVAQHDEADDPGWWDIVVGPGKAIDTRRYFVIC
                     ANLLGGCRGSTGPGSVNPATGKPFGRDFPTITVGDMVAAQRGLLDQLGIERLLAVVGG
                     SLGGQQALVWGTRYSDRVRGVVALATSARLSSQGLAFDVVGRNAILRDPHFHGGRYYD
                     RPQGPKVGLALARMIGHITYLSREAMREKFEADRLKPRDVAIEFEKRFSVGSYLGYQG
                     AKFVERFDANSYLTLSIAMDLFDLGVTAAELAAAFRPTRARWLVISFSSDWLFPPEQS
                     RDIVNALIANNAPVSYCEVQSPCGHDAFLLPHDLDRYGEMIRAFLDQLTPAPHAAPAA
                     RGEAYGPTSIFYQHRLDYERIAELVPPGASVLDLGCGSGTFLAGLRATNHRRLVGVEL
                     DEQKILTGIGRGLDIIQADLNRGLGAFADGQFDCVILSQTLQAVQDVERVLTEMVRIG
                     RTCIVSIPNFGYHRLRTMLAETGRAPKSAGVLHYEWYNTPNIRFFTIADFEDFCRQKG
                     IRVHRRITLDTEAKAEVFEHPNLNADLAIFVISRT"
     gene            2955..6218
                     /locus_tag="PKI07_07155"
     CDS             2955..6218
                     /locus_tag="PKI07_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOC50869.1"
                     /translation="MFSHRLLPAVETCVVLLLLLAGSARPAPPEDFPRFQVPGQEPAM
                     ATLRELFWLHYPAAGPKSTLWDEWLPDASLWPEVATGKQGDTLRAAWASALSGRILDP
                     EGYVASHQHASIAHQLGWPFPFWNQGQHGFGWHFSFKDTVGPVWRPAELSRLEGWALA
                     GARDGGTNDEGWRIEVTNRAAVVTAPPWRFQTFEAPFLQLRWRAAGLGAAQPFIEWTT
                     PARTNFTAARRMYFDPVTDDRISYTMIPMYRHAQWTGEVAQLRIGLGNPEPGHMTLQA
                     FFSQYDTRHNINCQNFVRGGAKYFWWTRDLGFLRANINRLRTALRYVMTEHQALERKV
                     VYTGWVGHDGRSGLGRDANGARVIHAGRGIGNNYWDLLPFGGLDAYATIQYYDAVRVM
                     AALEREIQQHPEWQIPGGVLSFDPAMLERHAEEVKTEGNRRFWNRETQRFNACEDVDG
                     QAYDYGLTFLNLEAVYYDFATPKHARAILSWINGDRVVAGDTSQGRDIYHWRFGPRAT
                     TRRNVDWYVWAWNTPENIPWGGQVQDGGAVLGFSFHDLMARLKVLGPDNAWQRLREVI
                     AWFDEVQAAGGYRKYYQEPREGSLQGGGTAGGLGLDQEFFESALVPQIMLKGFLGFAP
                     HADGFQLNPRLPRDWPELTVDRIQFQGRVWRIRATPEAIEIHKQGPGTEPLFLRLPGD
                     GWQAALLRADGSVIRRIRLRARESDGAFALPWEGAAAARLTKAADRPLKSAAEREAFF
                     RAQPSRPGEFRAAWIHSAYGIPGWGWEPTLATLHTNGFNAIFANLAWAGVAHYPSRIL
                     PVSPRVAEQGDQVAACLAACRQQGLELHVWKVNHNLLHAPPEFLAQLRAAGRTQKDVH
                     GGEVDWLCPSHPDNFALERDSMLELARAYDVDGLHFDYIRYPNGHACYCDGCRQRFER
                     DRGIRLARWPEDARTGPHAAAFGDWRREQITRLVRAVSQEAHRIKPAIKVSAAVFGNW
                     KSARQVVGQDALAWVDAGYLDFVCPMNYEPEDLEFAELLRQQVEAVNHRIPCYAGIGA
                     HTLSGPEQLVRQIQLARELGAEGFVLFSLTEKLAVRFLPPLRLGVTAAPPRSRSGAS"
     gene            6383..7432
                     /gene="gap"
                     /locus_tag="PKI07_07160"
     CDS             6383..7432
                     /gene="gap"
                     /locus_tag="PKI07_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOC50870.1"
                     /translation="MAVKVAINGFGRIGRLVFRAMVEQGLVGKDVEVVAVGDIVPADN
                     LAYLLKYDSIQGRFNGAIGSEKSTADRPDADVLVVNGKKIKVVSAKDPSQLPWKELGV
                     DVVIESTGLFTDVEKAKGHLTAGARKVVITAPAKGECPTIVMGVNEASYDPKAHHVLS
                     NASCTTNCLAPIVHVLLKEGFGVEEGLMTTVHSYTATQKVVDGPSKKDWKGGRSAALN
                     LIPSTTGAAKAVGLVLPAVKGKLTGMAFRVPTPTVSAVDLTVRTVKPTSYAEICAAMK
                     KASETYLKGILEYTEDEVVSSDFIHSPASSIFDKGAGIELNQRFFKLVSWYDNEWGYS
                     CRVSDLLKYMIQKGL"
     gene            complement(7568..8110)
                     /gene="msrA"
                     /locus_tag="PKI07_07165"
     CDS             complement(7568..8110)
                     /gene="msrA"
                     /locus_tag="PKI07_07165"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOC50871.1"
                     /translation="MNNPPPHTELATFGGGCFWCTEAVFQLLPGVKSVTSGYAGGHTA
                     NPTYREVCTGDTGHAEVVQVEYDPRQISYEKLLAAFWEAHDPTTLNRQGADAGTQYRS
                     IILYHNDAQKRAAEKAKAGAQPRFRAPIVTEIVPLERFYPAEAGHQNYYRANPNQPYC
                     RMVIQPKVEKFTRQLKTISP"
     gene            complement(8281..8859)
                     /locus_tag="PKI07_07170"
     CDS             complement(8281..8859)
                     /locus_tag="PKI07_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50872.1"
                     /translation="MKATLALIILAGFLGGTAWAGPEGVLKRARETRDQNNVRQDVAP
                     VRPPPPPTPARQPAAPAPSGPLQQSLARVRADLATIQANSPVTVTQKQQLAKDLLACA
                     QGASKPSAATVAALADSLVSALAQKPLPEASRQRLVSDLAAVLNPANLPPAQMQAIYN
                     DIQAIFQANGLPRTEAVKLADQARAIAAETRR"
     gene            9153..9242
                     /locus_tag="PKI07_07175"
     tRNA            9153..9242
                     /locus_tag="PKI07_07175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9187..9189,aa:Ser,seq:cga)
     gene            complement(9295..10860)
                     /locus_tag="PKI07_07180"
     CDS             complement(9295..10860)
                     /locus_tag="PKI07_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50873.1"
                     /translation="MMLCCQRVLGWGRFALLWGCVLVCGTARGAGVTLITHGYGGNVA
                     DWVIPMCNQIPLRSDFPGTNHTRYQFSITRSGNLLYTTVTLLGGGDPLTSDSGEIIIA
                     LDWSTLSSLGGASSSEIAAEAAEALLASNLIPALGGRRLAELPLHLIGHSRGASVVAE
                     MARELGAVGVWVEHQTTLDPYPVNLLGDPGTMRNYANVFYADNYWQNRDFPAGKSLAG
                     AYNRYLPELSGGYASGQNHSDTHLWYHGTVDGNTPAGDNLATITSAQRSTWWTALEQA
                     GTNAGFRYSLIGGGDRFSSQEPNGPGNGRINDGVNRKWDLGAGAGANRTSLPANSGAW
                     PNLVRLSLAGTNVVVSGQPVPIALYYQLGAAPAATGSLSLYLDLDANPYNENETELLN
                     CTLSGTGPNAVAALSTNAQPNPAAIMPRTYRLFGRLADGSRARYLYAPQTITLTPSLQ
                     APTLLDPVWQGNQFRFTLAAMPGQRIVIQRTPDFQTWTPVVTNVMRGPSAQIILSASS
                     ADPPGFYRALLTP"
     gene            complement(10907..12301)
                     /locus_tag="PKI07_07185"
     CDS             complement(10907..12301)
                     /locus_tag="PKI07_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOC50874.1"
                     /translation="MNCRLRLPIAMNLASDLSLLILCAALPAAAAGDRVRLITLDPGH
                     FHASLVQKSMYPQVSPEVRVYAPAGADLQEHLKRIESFNTRADQPTHWQEQVYTGPDF
                     LRRMLKDKAGNVVVLAGNNARKTDYINRAVGAGFNVLADKPMAITPAQFDQLRRAFRR
                     AAAQKVLLYDIMTERYEITSMLQRELARMPELFGVLQHGAPEQPAVVIESVHYFFKEV
                     AGKPLIRPAWFYDVRQQGEAIPDVGTHLVDAVQWICFPDQVLDWRRDIQVYHGRRWAT
                     RLTPEQFKLSTGQDRYPDYFKKDLGPDGALEVYANGEVGYALRGVHARITALWGFQAP
                     AGAGDTHYALLRGTRCHLRIQQGPEQNYQPALYVENPIGVSAAQFEAAVRAAVAKLDA
                     QWPGVGLKPAGASWEITIPAQYRVSHEQHFAQVTEKFLGCLAAGRLPDWEVPNMLAKY
                     YTTTQAYRLSHARK"
     gene            complement(12328..13725)
                     /locus_tag="PKI07_07190"
     CDS             complement(12328..13725)
                     /locus_tag="PKI07_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50875.1"
                     /translation="MNTSFPVSRRQFLQASLAATAGAAVFPTIVPASVLGADAPSKKI
                     QVGQIGCGRIALGMDLPGIIKHDIARVVAVCDLDSKRLANARKFVEDAYAKKPGSAKA
                     VAVNAYGNYRELLKDPQVDAVAISTPDHWHSEPIIAAALAGKDIYVQKPLTMTLVEGR
                     AVSDIVRARKRAFQIGSQQRSGTQFRLACELVRNGRIGKLRRVEIGLPVDPAGGRTEE
                     MPVPPNLDYDQWLGCTPKVPYTEDRVHSQRTTFERPGWLRIDSYCLGMITGWGSHHVD
                     IAHWGMGTEFTGPIAIEGKAEFPTEGLWNVHGPYHIEARYANGVLMVIDNTFPNGVKF
                     EGENGWIFVSRGSAKVTASDPATAFGKALDASDPKILNSKIGPNELHLPRSEDHHLNW
                     LQSIQTRQPAVTTPEEAHRSTSACILGWIAMKLGRRLRWNPEQETFVDDPQATALLSR
                     TQRAPYGIDRLLKKP"
     gene            complement(13752..14543)
                     /locus_tag="PKI07_07195"
     CDS             complement(13752..14543)
                     /locus_tag="PKI07_07195"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOC50876.1"
                     /translation="MDAMFRFRIWGIMLSLLVLLTASLPGATPERRVLVYTKNGKGYV
                     HDNLAASSAAIQKLGAAHNFAVDVSDDPAVFSDQNLKRYRALIFNNVNNEIFDTEEQK
                     AAFQRYIRAGGGFVGLHSACGAMRNWPWFWELVGGKFVRHPKLQTFTIKVKDSRDIST
                     AHLPKTFSWTDEFYFVDHMPKGRHILLAGDLTTLDDPAREKYPGRMFGDEFPLAWRRK
                     FEGARTWYTALGHKPEHYSDPKLTQHILGGILWAMGQSPRAARHP"
     gene            14804..16000
                     /locus_tag="PKI07_07200"
     CDS             14804..16000
                     /locus_tag="PKI07_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOC50877.1"
                     /translation="MNRRKRSWLAWISGVALAALLGGCAHPAGPGVKPTDDHSRAVDR
                     ALQSVKQDYAPDGRLAIFNVGLERLGRGLVLTGEVDRAEARIEILRAVERTGVKVADR
                     IKVLPEERLGEQVWGIGCLSVASGRLQPEHKAELGNQVLMGEIVRVWKRSSHPLYTWY
                     LVQTADGYLSWLHADTFVRCTREQAEAWRHAPLLIVTAMEGLVLEQPRADAPPVSDVV
                     LGNLVRKTGETGDWYQVELPDQRAGFLPRRAAQDYAAWARARRPTAENIEQTARRFLG
                     RPYLWGGNSTKGFDCSGLTKQVFFLNGIDLLRDSSKQAGQGVAVPLDPDLRQLQKGDL
                     LFFGRPARRSQPERVVHVGIYLGNKLFIHSSGRVHISSLDPQSPLRDEVRIRTLLRAR
                     RLLPAP"
     gene            16764..17981
                     /gene="thrC"
                     /locus_tag="PKI07_07205"
     CDS             16764..17981
                     /gene="thrC"
                     /locus_tag="PKI07_07205"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOC50878.1"
                     /translation="MLKLVSTLTRDEYPFAQLEEFAGNGEALEVAWPGLERARPRPGR
                     HLWERFAEFLPFERMDARASLGEGNTPLVEAGPRLATFSGLERLWLKNETQNPTGSFK
                     DRGSLTCVFMARERGEPTLATVSTGNMGHSIAAYAARSGLRALVFVPEFAPREKLLPM
                     TLHGATVIKVRAPDYALMKQTVLGLAGEFNLRIVSGNGPIRVEGYKLTAFELYEQTRG
                     ELPDYIAVPTSACGHLRGLFKGFRELQAAGLIRRCPRMIVVQAANNSPIVSALKQGAE
                     HVIPFANFHTVAEAITSGNPMGGDEIIAKARAHGWLAEDVTEAEILEAQRQLAAAGWF
                     VEPAAATTLGAVRKLRAAGRIAAGESVLLMLTGAGLKDLGVLEHQPFAVRESSLDALR
                     RDLARWFSDSSGR"
     gene            18074..18859
                     /locus_tag="PKI07_07210"
     CDS             18074..18859
                     /locus_tag="PKI07_07210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC50879.1"
                     /translation="MNAETVLITGASSGIGLALARCFAADGCRLVLVARKGDALEALA
                     AELRKTRKIQAQVIPADLARPEAPARLLAHLQAAGLKVDVLVNNAGFGAQGKFVELPL
                     ARQLEMIQLNLTTLTHLTGLLLPGMLERRRGGILNVASTAAFQPGPGMAVYYATKAYV
                     LSFTEALAEELAGTGLTITAVCPGPTTTNFGRAANMRTLGLVRKISMSAEAVARQGHR
                     GFRRGRVVVINGFRNQLPAFLVRGCPRAVVRKITRRLNNVKYP"
     gene            18867..19574
                     /locus_tag="PKI07_07215"
     CDS             18867..19574
                     /locus_tag="PKI07_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50880.1"
                     /translation="MPLRLASWLGILLAGSLAAAELAPAAPPPKSPTPGKTSRAAAEK
                     QECIRHLKAIYAAVQAYQADHHDLPNWLSDLVPRYLEDVNMLLCPVCRRTGRTESPSL
                     ADPKLPSSYLFEFCPVPLGAAATNAPTRTRREWKRRQMSLLGSDVPLVRCRHHKPMLN
                     LAFDGRIYESASVWELLFTNRVNVAELTPARLFAREAPPARPAPRADGKAGAAGPRKT
                     KAAPPRSEPAPPPREKK"
     gene            19651..21615
                     /locus_tag="PKI07_07220"
     CDS             19651..21615
                     /locus_tag="PKI07_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOC50881.1"
                     /translation="MLRLSGITKAYGGRVLFADATLQVNRQDRIGLVGPNGAGKTTLF
                     SIILGQESADAGQFDCERGISLGHLPQESAPVADETVLELATAITSEYTRLRRILKAW
                     EADHPAAALAPEEIHDDAHDRFRALGGYRLEARAKQILAGLGFRQADFDRPAREMSGG
                     WVMRTHLARLLTQEPDLLLLDEPTNHLDLEALLWFQEYLQSYPGAILVISHDREFLNQ
                     IVGSIVEIRQSRLIRYRGGYDEYLEQREAYEQQQLAAYKHQEREIARLLEFVNRFRAK
                     NTKAAQAQSKLKQIERMDKIAAPAGEERKLEFQFPQPRRGGLRAIKLEGIHHAYGENV
                     VYRGLDFEAARGQRIVLVGPNGAGKSTLLKLLAGVITPQAGARTPGHNVHVGYYSQYR
                     IEQLRPERTVLEEAFDTPRSLTEQFVRTLLGCFLFSGDDVFKRVEVLSGGEKSRLALV
                     KLLLDPPNLLLMDEPTTHLDLSSVDALTYALDQFEGTLIFISHDVYFIRTLANHVVHV
                     NAGRLTHYPGGYQYYLDRTRAVSARAGLTADGGPGVSALPGRPDSPARASSPAADRKE
                     QKRAAAEQRQARYRARKAQQERVDRLEQEIHELEARLADLVAELEQPGTYAKPGRPAA
                     INRELSEARRRLAELNPAWEREATRLAELE"
     gene            complement(21622..23790)
                     /locus_tag="PKI07_07225"
     CDS             complement(21622..23790)
                     /locus_tag="PKI07_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50882.1"
                     /translation="MRRRFCWRLSGAGLLHLGAIWILVTVVWGLANDRLSPASWRVPP
                     GYSGDALQMLTWIRAASEFDYLPFLSRVNPRLGAPYAANWDDYPMYEPVTTFLLGLVA
                     RYTCLGFAANLGILASFLASATSFYAACRLLRWRREWAAAGALLFAFTWYHSGRGLGH
                     LLLSYDYTVPLAVLSVWWLSAGRRARRGGAVFWLSAGTALALGMGNPYNLSMWAQFLV
                     LGLGVRYLLERRKADLATGAILLAVAAAGFLAVNAHTIGYGWAHGPNQLAVIRPYKQL
                     ELYALKPLELVLPPWQHRVSWLSDLAREYAAAAWVQGEMFSPYLGVVGVVALGWLTVE
                     FGRRALNLRATPRRLPSHAPLCLWVLLYAAVGGLNCLLGLVFGLLYFRGANRYSIFIS
                     ALVLFFLVSRMSRWARAWPRAASYALAAGVAGLGLLDQVPPPAKDATPALARAVENDQ
                     RFGRALEAALPAGAMIFQLPLMNFIDGDPIGGMLSYEHVRPYLWTERLRFSFGSVQGR
                     PREDWQAALAELPVAPCARQLEHYGFAGLYFNRKAYTDGAAAMLADLAQRGWTRFVAD
                     AAGEQVCVLLNPAPHPARPPADDAAQIVYRAHWSFGSYGLRDRGSRPARWACARVTSL
                     YFVNDRPQPCVFRVRAWVAAASARRVELCAGGQTLWSAQLEPGESQPLDLRVMARPGR
                     NYLRFQNDRPPAPPPGQPQAVRIAHCLVDLQITREPAASP"
     gene            complement(23892..24740)
                     /locus_tag="PKI07_07230"
     CDS             complement(23892..24740)
                     /locus_tag="PKI07_07230"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOC50883.1"
                     /translation="MKALATITVIGRDKTGVVARVTHFLFEQKGNIEALEEQVTRGQF
                     SMTVQTSWPGRSLDRRAVERGLAVLADELEMEITVRFTAPRRRQRMALMVTREPHCLE
                     KLLAAIQAGKLRKAEPALVLSNRRDLEPLARKARLPFVHLPWEDRAKAEQQALRVLAQ
                     HEVDFVVLARFMKILSANFVWRFKNKIINIHPSLLPSFPGPQAYRQAYEQGVKIIGVS
                     AHFVTMNLDEGPIIAQSCFAVRPGMTLRQIVAAGQKLEAQTLLKAVQLYLNKRLDVHW
                     GIVKEV"
     gene            complement(24822..25811)
                     /locus_tag="PKI07_07235"
     CDS             complement(24822..25811)
                     /locus_tag="PKI07_07235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOC50884.1"
                     /translation="MKTTTKSLKWLGAGLALLLAASLPAAGAAAQKARRPAAAPAPAK
                     SARPKPICRDPWLGAIVVDAATGKVLYEDQADAKGYPASVLKLMDLLIILEKIEQKQL
                     SLTDTVRVSAKAAQTGGSQVWLAEKESFTVDELLYALMIQSANDAAVALAEKVAGSTD
                     AFVALMNRRARELGMNNTVFSSVHGLPPTGGQKHDITTARDLSILCRQVLRCRDTLRY
                     TSARERPFRPNVPGKTVIMRTHNHLLKQVEGCDGLKTGYIAASGYSIAVTAARQGRRV
                     IVVVLDSATRTVRDARAAELVARGFAALSTPAPRAAPPAAAPPAATPAGNRPR"
BASE COUNT         4417 a         8530 c         8665 g         4574 t
ORIGIN      
        1 cttcgctttt tcggggatac cgggtggatt tttcttgaag ggacagccgg gcattgtcgt
       61 aaaagcgggc taagcccagg cgggcgcagg caggtgacgg cccgggggcg aagccggatc
      121 atcagagaaa aaaacttgaa gaatttgctt gcgcttgagt tgccgccttg ggtaacgtgg
      181 gccagactca aaagctaaga aagtatttgg tcaacggcat aacctgatta acccattata
      241 agtcaatgaa aagaattacc attacccgat tcattctggc ccatgtttct gcggtggcct
      301 tggtggccgc gcccagcgcc tgggccgaca cccttgcgca gtggaccttt gaaagcagtg
      361 gccccagcat tgctggcacg agtactacct tcggccccat cgcgcctgaa gttggcagcg
      421 gaaacgccta tggtgtccat gccagcgcgt caacggtgtg gtcttccccg gtaggcaacg
      481 gatcgcctga atcgttcagc gcgaacaact ggagtgtgaa cgactatttc gagtttacga
      541 ttccttcaac ggtcgggtac aaagacgtca gtattagcta tgatcaaatc ggcagtgcca
      601 ctggtccccg ggatttttca tttacctaca gcaccgatgg ctcgattttc atcacggtgg
      661 gcagttatca actcacgtct ggtgttagct ggtccggcac cattggcggc cggccagacc
      721 aactcagctt tcttaacatc agcgcgctgg acaactggaa cgggacgatt tatttccggg
      781 ttgttgatga cagcaccact tccataaatg gaggcaccgt tgcggcgggg ggcaccggcc
      841 gaattgataa tttcacggtc acggccacca tgattcccga gccgtcggcg ttggcgctgg
      901 tgggcctggg ggtggcgggc ctggttgctt tccgccgtcg caatggttaa ttagcttttg
      961 ttgagttttg accctggcgc gccgggatga gcccggcgcg cttttttgtt gcggcggtcc
     1021 cgccggggcg gcgattgccg ggtctggtgg cggcgcgacc cggcggcggt tcgggattcc
     1081 tgacgaattg ccaaatgggc gggcgtaggt caaattatcc ggcgtgacgc agaatccgtt
     1141 tgagagcagc gataccgtac ggcatgccca gccgttgcgg catgtgcggt tggcccgcct
     1201 ggcccagccg ctggcgctgg agatgggcgg ccagttggcg gaggtcacgc tggcttacga
     1261 aacctacggg gaattgaatg ccgcgcgcga caacgcggtt ctcatctgcc atgcgctgag
     1321 cggagattcg catgtggccc agcacgatga agccgatgac ccggggtggt gggacatcgt
     1381 cgtgggcccg ggcaaagcga ttgatacccg gcgttatttt gtcatttgcg cgaacctgct
     1441 ggggggctgc cgcggcagca ccgggccggg cagcgtcaat cccgccaccg gcaagccgtt
     1501 tggccgggat ttccccacca ttaccgtggg cgacatggtc gccgcgcagc ggggtttgct
     1561 ggatcaactg ggcatcgagc ggctgctggc ggtggtgggc ggctcgctgg gcgggcagca
     1621 ggcgctggtc tgggggacgc ggtattcgga ccgggtgcgc ggggtggtgg cgctggcgac
     1681 ctcggcgcgg ctgagcagcc aggggctggc gtttgacgtg gtggggcgca atgccattct
     1741 gcgcgacccg catttccacg gggggcgcta ctacgaccgg ccgcaggggc ccaaggtggg
     1801 gctggcgctg gcgcgcatga ttgggcatat cacctacctg tcgcgcgagg ccatgcgtga
     1861 aaagttcgag gccgaccggc tgaagccgcg cgatgtggcc atcgagtttg agaagcgctt
     1921 ttcggtgggc tcctacctgg gctaccaggg ggcgaagttc gtcgagcggt tcgacgccaa
     1981 cagctacctg acgctgtcca ttgcgatgga cttgttcgac ctgggcgtca cggcggcgga
     2041 gctggcggcg gcgtttcgcc ccacgcgcgc ccgctggctg gtgatcagct tctccagcga
     2101 ctggctgttt ccgccggagc agtcccgcga catcgtcaac gccctcatcg ccaataatgc
     2161 gccggtgagc tattgcgagg tgcaaagccc ctgcggccac gacgcgttcc tgctgcccca
     2221 cgatctggac cgctacggcg aaatgatccg cgcgtttctg gaccagctca cgcccgcgcc
     2281 gcacgccgcg ccggcggccc ggggggaggc gtatgggccg accagcattt tctaccagca
     2341 tcggctggat tacgagcgca ttgccgagct ggttccgccg ggcgccagcg tgctcgattt
     2401 gggctgcggc tcgggcacgt tcctggccgg cctgcgcgcg accaaccacc ggcgcctggt
     2461 cggcgtcgag ctggacgagc agaagattct caccggcatt ggccgcggcc tggacatcat
     2521 ccaggcggat ttgaaccggg gccttggcgc ctttgccgac gggcagtttg actgcgtgat
     2581 cctgtcgcag accctgcagg cggtgcagga tgtggagcgg gtgctgacgg aaatggtgcg
     2641 catcgggcgc acctgcattg tgagcattcc caacttcggg tatcaccgcc tccgcaccat
     2701 gctggccgag accgggcggg cccccaagtc cgccggcgtg ctgcattacg aatggtataa
     2761 cacgcccaac atccgcttct tcaccattgc cgattttgag gacttctgcc gccagaaagg
     2821 catccgggtg catcggcgca tcacgctgga taccgaagcc aaggccgagg tgttcgagca
     2881 ccccaacctg aacgccgacc tggccatctt cgtcatcagc cggacctaag ccccccccgc
     2941 aacctctttt tcccgtgttt tcccatcgcc tgcttcccgc cgttgaaacc tgcgtcgtcc
     3001 tgttgttgct gctggccggt tcggcccgcc cggcgccgcc ggaggatttt ccccgcttcc
     3061 aggtgcccgg ccaggagccg gcgatggcaa cgctgcgcga gttgttctgg ctgcattatc
     3121 ccgccgcggg gcccaaatcc acgctgtggg acgagtggct cccggatgcc tcgctgtggc
     3181 cggaggtcgc caccggcaag cagggcgaca ccctgcgcgc ggcgtgggct tcggcgttaa
     3241 gcggccgcat cctggacccg gagggctacg tggcctcgca tcagcacgcg agcatcgccc
     3301 atcagctcgg ctggccgttt cctttttgga accagggcca gcacggcttc ggctggcatt
     3361 tctcgttcaa ggacaccgtg ggcccggtct ggcggcccgc cgagctgagc cggctggagg
     3421 gctgggcgct ggccggggcg cgcgacggcg gcacgaatga cgagggctgg cgaattgagg
     3481 tcaccaaccg cgccgcggtg gtgacggcgc cgccctggag gttccaaacc ttcgaggcgc
     3541 ccttcctcca gcttcgctgg cgggcggccg gcctgggcgc ggcgcagccg tttattgagt
     3601 ggaccacgcc ggcccggacg aactttaccg ccgcgcgccg gatgtatttc gatccggtga
     3661 cggacgaccg gatcagctat acgatgattc ccatgtatcg gcatgcgcag tggacggggg
     3721 aggtggcgca actgcgcatt gggctgggca acccggagcc gggccacatg accctgcagg
     3781 cgtttttctc gcaatacgac acccggcaca acatcaactg ccagaacttc gtgcgcggcg
     3841 gcgccaagta tttctggtgg acccgcgacc tggggttcct gcgggccaac atcaaccgcc
     3901 tgcgcaccgc cctgcgctat gtcatgaccg agcaccaggc gctggagcgc aaggtggttt
     3961 ataccggctg ggtgggccat gacggacgga gcgggctggg gcgggacgcg aacggcgccc
     4021 gggtgattca tgccgggcgg ggcatcggca ataactactg ggatttgctg ccgtttggcg
     4081 ggctggatgc ctacgccacg attcaatact acgacgccgt ccgcgtcatg gccgccctcg
     4141 aacgcgagat tcagcagcat cccgaatggc aaatccccgg cggggtgctg agcttcgacc
     4201 cggcgatgct ggagcgccat gccgaggagg tgaagaccga gggcaaccgc cggttctgga
     4261 accgcgagac gcagcggttc aatgcgtgcg aggatgtgga cggccaggcc tacgactacg
     4321 ggctgacctt tctcaacctc gaggcggtct attatgattt tgccacgccg aaacacgccc
     4381 gcgccatcct gagctggatc aacggggatc gcgtggtggc gggcgatacg tcgcagggcc
     4441 gggatattta ccattggcgc ttcgggccgc gggccacgac gcggcgcaac gtggactggt
     4501 atgtctgggc ctggaacacg ccggaaaata ttccctgggg cggacaggtg caggacggcg
     4561 gggcggtgct gggtttttcc ttccatgatc tgatggcccg cctgaaggtg ctcgggccgg
     4621 acaacgcctg gcagcggttg cgcgaggtga tcgcctggtt cgacgaggtg caggcggcgg
     4681 gcggctaccg caagtattac caggaaccgc gcgaggggtc tttgcagggc ggcggcaccg
     4741 cgggcggcct ggggcttgac caggagtttt tcgaaagcgc gctggtgccc cagatcatgc
     4801 tcaagggctt cctcggcttt gccccgcacg ccgatggctt tcagctcaat ccccggctgc
     4861 cccgggactg gccggagttg accgtggatc ggatccagtt ccagggccgg gtctggcgca
     4921 ttcgggccac gcccgaggcc attgaaatcc ataaacaggg gccggggacc gagccgctgt
     4981 tcctgcgcct gcccggggac ggctggcagg ccgcgctgct gcgcgcggac gggtcggtaa
     5041 tccggcggat ccggctccgc gcgcgggaaa gcgacggcgc gttcgcgctt ccctgggagg
     5101 gcgccgccgc ggcgcgcctg acaaaggccg cggaccgtcc gctgaaaagc gccgccgagc
     5161 gcgaggcctt tttccgcgcg caaccttccc gccccggcga gtttcgcgcc gcctggattc
     5221 actcggccta cggcatcccg ggttggggct gggagcccac cctcgccacg ctccacacca
     5281 acgggttcaa tgcgatcttc gccaacctgg cctgggccgg ggtggctcat tacccgagcc
     5341 ggattttgcc ggtgagcccc cgggtggccg agcagggcga ccaggtggcg gcgtgcctcg
     5401 cggcgtgccg ccagcagggc ctcgaactgc atgtctggaa ggtcaaccat aacctgctgc
     5461 acgcgccgcc cgaattcctc gcgcaactca gggccgccgg ccgcacgcaa aaagatgtcc
     5521 acggcggcga ggtggactgg ctttgcccgt ctcatcccga caacttcgcc ctggagcggg
     5581 attcgatgct cgaactggcg cgcgcgtatg acgtggacgg ccttcacttc gattatattc
     5641 gctacccgaa cggccacgcc tgctactgcg acggctgccg ccagcgcttc gagcgggacc
     5701 gcggcatccg gctggcgcgg tggccggagg acgcgcgcac cgggccgcac gccgccgctt
     5761 ttggcgactg gcggcgcgag caaatcacgc ggctggtgcg cgccgtgagc caggaggcgc
     5821 atcggatcaa gcccgcgatc aaggtctcgg ccgcggtgtt cggcaactgg aaaagcgccc
     5881 gccaggtggt gggccaggac gcgctggcct gggttgacgc cgggtatctc gactttgtct
     5941 gcccgatgaa ttatgagccg gaggacctgg agttcgccga actgctgcgc cagcaggtgg
     6001 aggcggtcaa ccaccggatt ccctgctacg cgggaatcgg cgcgcacacg ctgtccgggc
     6061 cggagcagct cgtccgccaa atccagttgg cgcgcgagtt gggcgcggag ggctttgtgc
     6121 tgttcagcct gacggagaag ctggccgtgc ggtttctgcc cccgctgcgg ctcggggtca
     6181 cggccgcgcc gccccggagc cggagcggcg cctcctgagt ccgcgcggat cgccggcccg
     6241 gaaggcatgc gcccggggcg cgcgcggttg cggagcggct tgggtttttt gggccgccgc
     6301 gcttgttggt ttgcctattg acctccggag cgcgggtgat atgcttagct gttctatttt
     6361 acatattatc tgaaaacgaa ttatggcagt caaagttgca attaatgggt tcggccgcat
     6421 tggtcgcctg gtgttccggg cgatggtcga gcaaggtttg gtcgggaaag acgtggaagt
     6481 cgttgccgtt ggcgatattg tgccggcgga taacctcgcg tatctcctga agtatgattc
     6541 catccagggc cgcttcaacg gcgccatcgg gtcggagaag tccaccgccg accggccgga
     6601 cgcggatgtg ctcgtcgtca acggcaaaaa gatcaaggtg gtcagcgcca aagatccttc
     6661 ccagttgccc tggaaagaat tgggcgtgga cgtggtgatc gagtccaccg gcctgtttac
     6721 cgacgttgag aaagccaagg ggcatctcac tgccggggcc aggaaggtgg tcattaccgc
     6781 tcccgccaag ggcgagtgtc cgaccattgt catgggcgtg aacgaagcca gctacgaccc
     6841 gaaagcgcat catgttctgt ccaacgcctc ctgcaccacc aactgcctgg cgcccatcgt
     6901 gcatgtgctg ctcaaggaag ggtttggtgt ggaagagggg ctgatgacca ccgtccattc
     6961 ctataccgcc acgcagaagg tcgtggacgg cccgagcaag aaggactgga aaggcggccg
     7021 cagcgcggcg ttgaacctga tcccctcgac taccggcgcc gccaaggccg tgggcctggt
     7081 gctgccggcg gtcaaaggca aactgaccgg catggccttc cgcgtgccga cgcccaccgt
     7141 ttcggccgtg gacttgacgg tgcggacggt caaaccgacc agctacgcgg aaatctgcgc
     7201 ggcgatgaag aaggccagcg agacctacct caagggcatc ctcgaataca ccgaggacga
     7261 agtggtcagt tcggacttca tccacagccc ggcttcctcg atcttcgata aaggggccgg
     7321 catcgagctg aaccagcgct tcttcaagct ggtgagctgg tacgacaacg agtggggcta
     7381 tagctgccgc gtttcggacc tgctaaagta tatgatccaa aagggtcttt gattttggtt
     7441 gggagttggg tgaatcaagg cggctcggca acgggccgcc tttgttattt ccgccatagt
     7501 tcaacccgcg cgcccggctt cgccgggagc gtgcgtcccg aggcgggccg tttatcgtta
     7561 tttccggcta cggggagatg gttttgagct ggcgcgtgaa cttttccact ttgggctgga
     7621 tgaccatccg gcagtagggc tggttcgggt tggcgcggta ataattctgg tggccggctt
     7681 cggcggggta gaaccgttca agcggcacga tctccgtcac tatcggcgcc cggaagcgcg
     7741 gttgcgcccc ggctttggct ttctccgcgg cgcgtttttg cgcgtcgttg tggtagagga
     7801 tgatggagcg gtattgcgtg ccggcgtcag ccccctggcg gttcagcgtc gtggggtcat
     7861 gcgcctccca aaaggccgcc agcagctttt cgtaggaaat ctgccgcggg tcatattcaa
     7921 cctggaccac ttcggcatgg ccggtgtcgc cggtgcagac ctcccgataa gtcgggtttg
     7981 ccgtgtggcc gcccgcataa ccgctggtga cggacttgac tcccgggagc aactgaaaca
     8041 ctgcttcggt gcaccagaaa cagccgccgc cgaacgtggc gagttcggtg tgggggggtg
     8101 ggttgttcat aatattcgtg cgcgtcacct ccgcggttgc ctccgcgagc agccagccca
     8161 gggccagcag agccatcggc gcgagggtcg aaagccgggg gcggctggac ccgcggcgcg
     8221 gatgtccgat tcgtttcatc agcaatcagg aacgcctctc ctccggggag gagctggagc
     8281 ttatctccgg gtttcggcgg cgatcgcccg ggcctggtcg gcgagtttta ccgcttcggt
     8341 gcgcggcagg ccgttggctt gaaagatggc ctggatgtcg ttgtaaatag cctgcatttg
     8401 ggcggggggc aggttggccg ggttaagcac cgcggccagg tctgacacca accgttggcg
     8461 gctcgcctcg ggcagcggct tttgcgccag ggctgagacg aggctgtcgg ccagggcggc
     8521 cacggtcgcc gccgatggtt tgctggcgcc ctgcgcgcag gcgagtaggt ctttggcaag
     8581 ctgctgcttt tgcgtgacgg tgaccgggga attcgcctgg atggtcgcca aatccgcccg
     8641 cacccgggcc aggctctgct gcaagggccc cgaaggcgcc ggggcggcgg gttggcgcgc
     8701 tggcgtgggc ggcggcggtg gccggaccgg cgcgacatcc tgtcggacgt tattctgatc
     8761 gcgcgtttcc cgggcgcgct tgagaacgcc ttcggggcca gcccaggcgg ttcccccgag
     8821 aaacccggcc aaaataatca gggcgagggt tgctttcata agttgatcgg tgctgccatc
     8881 aacctgtgcc gccccaatgg ctttgtcaac cctccctccc gggtgacgga tgcgagcgag
     8941 gggcatatcc cgagaagaat ccctgcgggg cggatgccgt ccggggcggc tcagctttcc
     9001 ggctccggga aagcggggcg tttcgggatg aagcgctccg ggtcggtttg cgtattgcca
     9061 gaatgttcag gcccaaaagg attggcgcac ctgatgaatc cagttgccgt cgccgtaaat
     9121 tgcggcacaa tatatctcgg ttggcctgcc gcggaagggt ggctgagtgg ttaaaggcac
     9181 ctgactcgaa atcaggcgta ggggtaaccc taccgtgggt tcgaatccca ccccttccgc
     9241 cagttctatg acgcgcaatg cgccctgcaa gcttgctccc cccttcgccc gcactcaagg
     9301 cgtcagcagg gcgcggtaga agccgggcgg gtcggcggag ctggcggata aaataatctg
     9361 cgcggaaggc ccccgcatca cattggtgac cacgggcgtc caggtttgga agtcgggagt
     9421 gcgctgaatc acgatgcgct gccccggcat ggccgccagg gtgaagcgga actggttgcc
     9481 ctgccaaacc gggtcgagca gggttggcgc ctgcagactg ggggtcaggg tgatggtttg
     9541 gggcgcatac aaataccgtg cgcgggagcc gtcggccagc cgcccaaaca ggcggtaggt
     9601 tcgcggcata atcgcggcgg ggttgggctg cgcattagtg gaaagagcgg cgaccgcatt
     9661 ggggccggtg ccgctgagcg tgcagttcag gagttcggtt tcgttttcgt tgtaggggtt
     9721 ggcatccagg tccaagtaca ggctcaggct gccggtggcg gcgggggccg cgccaagctg
     9781 gtagtagagc gcaatcggca ctggctggcc cgagaccacc acgtttgttc cggccaggct
     9841 caggcggacc aggttcggcc aggcgccgct gttcgccggg aggctggtcc ggttggcgcc
     9901 cgcgccagcc cccagatccc acttgcggtt caccccatcg ttgatgcgtc cgttgccggg
     9961 gccgtttggc tcctgggaac tgaaacggtc gccgccgccg atcaggctgt agcgaaagcc
    10021 ggcattggtg ccggcctgct cgagagctgt ccaccaggtg ctccgctggg cgctggtgat
    10081 ggtggccagg ttatctcccg ccggggtatt cccatccacg gtgccgtggt accacaggtg
    10141 ggtatcgcta tggttttggc cggaagcata accgccgctc aactccggca ggtagcgatt
    10201 gtaggcgccg gccagcgatt tgccagcggg gaaatccctg ttttgccaat agttgtcggc
    10261 atagaagacg ttggcgtagt ttcgcatggt gccgggatca cccagaagat tcaccgggta
    10321 gggatccagc gtggtttgat gctccaccca gacccccacc gcgcccagct cgcgcgccat
    10381 ttccgccacg accgaagcgc cgcggctatg cccgatcaga tggagcggca gttccgccag
    10441 gcgccggccg cccagggccg gaatcaggtt cgaggccagc aacgcctcgg ccgcctcggc
    10501 cgcaatctcc gagctggagg ctccgccgag cgaggaaagc gtggaccagt ccagggcgat
    10561 gatgatttcg cccgagtccg acgtgagcgg atccccaccg ccgagcaggg tcacggtggt
    10621 atagaggaga ttgccgctgc gggtaatgga aaactggtag cgggtgtggt tcgtgcccgg
    10681 gaaatcgctc cgcaggggga tctgattgca catgggaatc acccagtcgg ccacgttgcc
    10741 tccatatccg tgcgtgatga gcgtgacccc cgccccccgg gcggtgccgc agaccagcac
    10801 gcagccccac agcagggcga atcgtcccca accgaggacg cgctggcagc aaagcatcac
    10861 tgcttttaat caaccccagg ccgccccggc tgtcaaacgg cgctggctat tttcgggcgt
    10921 ggctgaggcg gtaagcctgg gtggtggtgt agtacttggc gagcatgttg ggcacttccc
    10981 agtcgggcag ccggcccgcc gccaggcagc ccaggaactt ctcggtcacc tgggcgaagt
    11041 gctgctcgtg gctcacgcga tattgggcgg gaatggtgat ttcccacgag gcgcccgcgg
    11101 gcttgagccc cacgcccggc cattgcgcat cgagtttggc aaccgcggcg cgaaccgccg
    11161 cctcaaattg ggcggcgctg acaccgatcg ggttctcgac ataaagggcg ggctggtagt
    11221 tttgttccgg gccctgctgg atgcgcaggt ggcagcgcgt gccgcgcagc agcgcgtaat
    11281 gggtgtcgcc cgcgccggcg ggggcctgga acccccagag cgcggtgatc cgggcgtgga
    11341 cgccgcgcaa cgcgtagccg acctcgccgt tggcatacac ctcgagcgcg ccgtccgggc
    11401 cgaggtcttt cttgaaataa tccgggtaac ggtcctggcc cgtggagagc ttgaactgct
    11461 ccggagtgag ccgggtggcc cagcggcgcc cgtgatacac ttgaatatcc cggcgccaat
    11521 ccagcacctg gtcgggaaag caaatccatt gcaccgcgtc caccaggtgc gtgccgacat
    11581 ccggaatggc ctccccctgc tggcgcacat catagaacca ggcgggccga atcagcggct
    11641 tgcccgccac ctctttgaag aagtaatgca cgctttcaat gaccaccgcg ggctgctccg
    11701 gggcgccgtg ctgcaagacg ccgaacaatt ccggcatgcg cgccagctcg cgctggagca
    11761 tgctggtgat ttcgtagcgc tcggtcatga tgtcgtagag cagcaccttt tgcgcggcgg
    11821 cgcggcggaa ggcccggcga agctgatcaa actgggcggg cgtgatggcc atgggtttgt
    11881 cggccagcac gttgaacccg gcccccaccg cgcggttgat gtaatccgtc ttgcgggcgt
    11941 tgttgcccgc cagcaccacg acgttgcccg ccttgtcctt gagcatgcgc cgcaggaaat
    12001 cagggccggt atagacctgc tcctgccagt gcgtgggctg gtccgcgcgg gtgttaaagc
    12061 tctcgatgcg cttgaggtgc tcctgcaagt ccgcgcccgc gggcgcataa acccgcacct
    12121 ccgggctcac ttgcggatac atgctctttt gcaccaggct ggcgtggaaa tggccggggt
    12181 ccagcgtgat gagccggaca cggtcgccgg ccgccgcggc ggggagcgcg gcgcatagga
    12241 tgagcaggct gaggtcggag gcaaggttca tggcaatcgg cagccggagg cggcaattca
    12301 tcaatttcgg ccggcaattc ccgggcttca aggctttttg agcagccggt caatgccgta
    12361 gggcgcccgc tgcgtccggg acagcagggc ggtggcctgc gggtcatcca cgaaggtttc
    12421 ctgttcggga ttccagcgga gccggcgccc cagcttcatg gcaatccagc ccaggatgca
    12481 ggcgctggtc gagcggtgcg cctcctcggg cgtggtgacg gcgggctggc gggtttgaat
    12541 gctctggagc cagttcaggt ggtggtcttc gctcctgggc aggtggagtt cgttcggccc
    12601 gatcttcgag ttgaggatct tgggatcgct ggcatccagc gctttgccaa aggcggtggc
    12661 cgggtcgctg gcggtgacct tggcgctgcc ccggctgacg aagatccagc cgttctcgcc
    12721 ttcgaacttc acgccgttgg ggaaggtatt gtcaatcacc atgagcacgc cgttggcgta
    12781 gcgggcttcg atatggtaag ggccgtggac gttccacaaa ccctcggtgg gaaactcggc
    12841 cttgccctcg atggcaatcg ggccggtgaa ctccgtgccc atgccccagt gcgcgatatc
    12901 cacatggtgc gatccccagc cggtgatcat gccgaggcaa taggaatcaa tgcgcagcca
    12961 cccgggccgc tcgaaggtcg tgcgctggga atggacgcgg tcttcggtgt agggcacctt
    13021 cggcgtgcag cccagccatt ggtcgtaatc caggttgggc ggaaccggca tctcctccgt
    13081 gcggccaccc gcgggatcca ccggcaggcc gatttcgacc cggcgcagct tgccgatccg
    13141 cccgttgcgc accagctcgc aggcgaggcg aaactgcgtg cccgagcgct gctggctgcc
    13201 gatctggaag gcgcgtttcc gggcgcggac aatatcgctg accgcgcggc cttccacgag
    13261 ggtcatggtg agcggcttct gcacgtaaat atccttgccg gccagcgcgg cggcaatgat
    13321 cggctcgcta tgccagtggt cgggcgtgct gatggccacc gcgtccactt gcgggtcttt
    13381 caacagctcg cggtaattgc cgtaggcgtt caccgccacc gcctttgcgc tgcccggctt
    13441 tttggcgtag gcgtcttcga caaacttgcg ggcgttcgcc agccgcttgg aatccaggtc
    13501 gcaaaccgcc accacgcggg cgatgtcgtg cttgataatg ccgggcaggt ccatgcccag
    13561 ggcgatgcgt ccgcagccga tctggccgac ctgaatcttc ttgctcggcg cgtccgcccc
    13621 caggacagaa gcgggcacga tggttgggaa gaccgccgcg ccagccgttg cggccagcga
    13681 agcctggagg aattgccgtc gggataccgg gaatgatgtg ttcataaata tcgaagggga
    13741 gccagggagg gttatgggtg acgggcggcg cgcgggcttt ggcccatcgc ccacaggatg
    13801 ccgccgagaa tatgctgcgt gagtttgggg tcggaataat gctcgggctt atgccccaac
    13861 gccgtgtacc aggtgcgcgc gccctcgaac ttgcggcgcc acgccagcgg aaactcgtcg
    13921 ccgaacatcc ggcccggata tttctcccgg gccggatcgt ccagcgtggt caggtcgccc
    13981 gccagcagga tatgccggcc cttgggcatg tggtccacga aatagaactc gtccgtccag
    14041 gaaaaagtct tcggcagatg cgcggtcgaa atatcccggg aatccttcac tttgatggtg
    14101 aacgtctgca gtttgggatg gcgcacgaac ttgccgccga ccagctccca gaaccacggc
    14161 cagttgcgca tcgcgccgca ggcggaatga agccccacaa acccgccccc ggcccggata
    14221 taacgctgaa acgccgcctt ctgctcttcg gtgtcgaaaa tctcattatt gacgttgttg
    14281 aagatcaacg cccggtaccg tttcaggttc tgatcggaga acaccgccgg gtcatccgag
    14341 acatccacgg cgaagttatg cgcggcgccg agcttttgaa tcgccgcgga gctcgccgcg
    14401 aggttgtcgt ggacatatcc cttgccgttt ttggtataaa ccagcacccg gcgttccggg
    14461 gtcgcgcccg gcaggctggc ggtcaggagc accaacaggc tcagcatgat tccccatatc
    14521 ctgaagcgaa acatggcatc cataagcaac taccgcagta atcgagcgtt caccaaagcg
    14581 cccccgccca cagcgagcgc gcattcttat tatctctccc cgcctcgctg gcgcaagggt
    14641 aaatccgcag aatgggaagc aattcttttc ccggcgcccg ccctccggcc cgccggccgc
    14701 ttggccgaag tccccggccc ccgtggcggc gccacgcggc ttttgtgttt atgctggctt
    14761 tcgggccgga ttccggcttg tttatcgcct tatatctgaa cttgtgaata gacgaaaacg
    14821 aagctggctg gcttggattt ccggggtggc actggcggcc ctgctggggg gctgcgcgca
    14881 tccggcgggc ccgggagtca aacccacaga cgatcactcc cgcgcggttg accgcgcgct
    14941 ccagagcgta aagcaggatt acgccccgga tggccgcctg gcaatcttca acgtcggcct
    15001 tgagcgcctg gggcgcgggc tggtcctgac cggcgaagtg gatcgggccg aagcccgaat
    15061 cgaaatcctc cgcgccgtcg agcgcacggg ggtcaaggtc gcggaccgca tcaaggtgct
    15121 gccggaggaa cgattgggcg aacaagtatg gggcattggc tgcctgagcg tcgccagcgg
    15181 ccggctgcag cccgagcaca aagcggaact gggcaaccag gtcctgatgg gcgaaatcgt
    15241 gcgcgtctgg aaacgctcct ctcacccgct ctatacgtgg tacctcgttc aaaccgccga
    15301 tggctacctc tcctggctgc acgcggacac gtttgtccgc tgcacgcggg agcaggcgga
    15361 ggcgtggcgg catgcgcccc tgctgatcgt cacggccatg gaaggcctcg tcctggaaca
    15421 gccccgcgcg gacgcgccgc cggtctccga tgtggtgctg ggcaacctcg tgcgcaaaac
    15481 cggcgaaacg ggcgactggt atcaagtcga gctgcccgac cagcgtgcgg ggttcctgcc
    15541 ccggcgcgcg gcccaggact acgccgcctg ggcgcgggcg cggcggccca ccgccgagaa
    15601 catcgaacaa acggcgcggc gatttctggg ccggccctac ctttggggcg gcaactcgac
    15661 caagggcttt gactgttccg gcctgaccaa gcaggtcttc tttctgaacg gcattgattt
    15721 gctgcgtgat tccagcaaac aggcgggcca gggcgtggct gtgccgctgg acccggatct
    15781 gcggcaactc cagaaaggcg acctgctgtt ctttgggcgc ccggcccgcc gcagccagcc
    15841 ggagcgggtg gtgcatgtgg gcatatacct cggcaacaaa cttttcatcc attcctccgg
    15901 ccgggtgcat atcagcagcc tcgaccccca atctcccctg cgtgacgaag tgcgcatccg
    15961 caccctcctg cgcgcccgcc gactgctccc cgccccataa gggcggcggc ggccggggcc
    16021 tcgccccgcc cgtggccggg cgtgcacggt ttagccggat cggttcagat ttcgcctcta
    16081 attcggccct gcggcacgca taaacctctg attttacaga cgaaaggcat tcgcagagcc
    16141 aaaagccgtc cggatgtccc ccctctcttg aaaaaccgcc gtttgtcagc ctcggcttta
    16201 taactgactg tttttacaga gtttagtaat aatcctccca ttccttctcc tcgtttcggc
    16261 ctttccgggt atccatattc attatccaga taccctgctt ttaccctgcc atcaggctgt
    16321 gtacaagggg tgtacaaggg atgctcaccg ggagttcatg ggattttagc tggtacaccc
    16381 gatgggcatc ccttgtacac cccttgtaca cagcctgatg gcagggtaaa tccaagggat
    16441 gttcaaggca tggtcatggt gggccgaagc cggcaggatt ccgattttgg ccttattttg
    16501 cccctctcga tgcctaaatc cttgatttta tcggcgaaag gctcgggatt cggcagctat
    16561 acccttgaaa ttgctaaaga aagggttcgc caagggttcg gtttgggcgg tcaggcccgt
    16621 tcaggctccg atggaacggg ggcgggaggc ggaacagcct aactgaatcg ttatcgttta
    16681 gtcatcatgg gcttctttat tcgcccgctt gacaggccgc gccggatgct ttccactcag
    16741 aagccaatac gcaagtggcg cccatgctaa aactcgtctc gaccctcacc cgcgatgaat
    16801 accctttcgc gcagttggag gaattcgccg ggaacggcga ggcgctggag gttgcctggc
    16861 ccggcctcga gcgggcgcga ccgcggcccg gccgccacct gtgggaacgg tttgcggagt
    16921 tccttccgtt cgagcgcatg gatgcgcgcg cgtcgctggg agagggcaat accccgctgg
    16981 tggaagcggg gccccggctg gccacgttca gcggcctcga acggctttgg ctgaagaacg
    17041 agacgcagaa ccccaccggg agcttcaaag accgcggctc gctgacctgt gtgttcatgg
    17101 cgcgggagcg cggcgagccg actctggcga ccgtctcgac gggcaacatg ggccattcca
    17161 tcgccgccta cgccgcccgg tccggcctcc gggcgctggt cttcgtcccg gagtttgcgc
    17221 cgcgggagaa actcctgccc atgaccctgc acggcgccac ggtgatcaaa gtccgcgcgc
    17281 cggactacgc gctgatgaag caaaccgtgc tcggcctggc cggggagttc aacctgcgca
    17341 tcgtctccgg caacggcccc attcgcgtcg agggctacaa actgaccgcc ttcgagctgt
    17401 atgagcagac acgcggcgag ctgccggatt acatcgccgt gccgacctcg gcctgcggac
    17461 acctgcgcgg gttattcaag ggtttccgcg aactccaggc cgcggggctg attcgccggt
    17521 gtccgcgcat gattgtcgtg caggcggcca acaacagccc catcgtcagc gcccttaaac
    17581 agggagcgga gcacgtcatc ccgtttgcca acttccatac cgtggccgag gccattacct
    17641 ccggcaaccc gatggggggc gatgaaatca tcgccaaagc ccgcgcccac ggctggctgg
    17701 ccgaggacgt gacggaagcg gagatattgg aggcgcaacg ccagttggcg gcagccggct
    17761 ggttcgtcga gccggccgcc gccacgaccc tgggcgcggt tcggaagctg cgcgccgccg
    17821 gccggatcgc cgccggcgaa tccgttttgc tcatgctgac gggcgccggg ctcaaagacc
    17881 tgggcgtgct ggagcatcag ccgttcgcgg tcagggaaag cagcctcgac gctctgcgcc
    17941 gggatttggc gcgctggttt tccgattcct ccgggcgctg atcccggggc ccggcccggg
    18001 aggcgatccc tcccgccgcg ccgcgctttc tgcgcgattg ccaaacccgg cggcgtcagg
    18061 ttagctgagg cccatgaatg ctgagacggt tctcatcacc ggcgcttcgt cgggcattgg
    18121 gctggccctg gcccggtgct ttgcggcgga cggctgccgg ctggtgctgg tcgcgcgcaa
    18181 gggggatgcg ctcgaggcgt tggcggcgga gctgcgcaaa acgcgcaaga ttcaggcgca
    18241 ggtcatcccc gccgatctgg cccgcccgga agccccggcc cggctgctgg cgcacctgca
    18301 ggcggcgggc ctcaaagtgg acgtgctggt gaacaacgcc gggtttggcg cgcaggggaa
    18361 gtttgtcgaa ctgcccctgg cccggcagtt ggagatgatt cagctcaacc tgaccaccct
    18421 gacccacctc accggcctgc tgcttccggg catgctcgaa cgccggcgcg gcggtatcct
    18481 taacgtcgct tcgaccgccg cctttcagcc cggccccggc atggcggttt actacgcgac
    18541 gaaggcgtat gtgctctcgt tcaccgaagc cctggccgaa gaactggccg gaacgggcct
    18601 gaccatcacc gcggtatgcc cggggccgac gacgacgaac ttcggccggg cggccaatat
    18661 gcgcacgctg gggctggtga ggaaaatctc catgtccgcc gaagccgtcg cccggcaggg
    18721 tcaccgcggc ttccgccgcg gccgggtggt ggtcatcaac ggctttcgca accagctccc
    18781 ggcgtttctg gttcgcggat gcccgcgcgc cgttgtccgc aagatcaccc ggcgcttgaa
    18841 taacgtgaaa tacccctgat tccgccatgc cgctccgcct tgcatcctgg ctcggcatcc
    18901 tgctggccgg ctccctcgcc gccgccgagc tggctcccgc cgcgcccccg cccaagtctc
    18961 cgacccccgg gaaaaccagc cgcgccgcgg cggaaaagca ggaatgcatc cggcatctga
    19021 aggcgatcta cgcggccgtc caggcttatc aagccgatca tcacgacctg cccaactggc
    19081 tgtcggattt agtgccccgc tatcttgagg atgtgaacat gctgctctgc ccggtctgcc
    19141 ggcgcacggg ccggacggaa tcgccgtcgc tggcggatcc caaactgccg agctcctacc
    19201 tgttcgagtt ctgcccggtg ccgctgggcg ccgccgccac caacgcgccg acccggacgc
    19261 gccgcgagtg gaaacgccgc cagatgagcc tgctgggttc ggatgtgccc ctggttcgct
    19321 gccgccatca taagcccatg ctgaacctgg cgtttgacgg caggatctat gagagcgcct
    19381 cggtctggga gctgctgttt accaaccggg tcaacgtcgc ggagctgacc cccgcccgcc
    19441 tcttcgcccg cgaagctccc ccggcgcgcc ccgccccccg ggcggacggc aaggccgggg
    19501 cggccggacc gcgcaaaaca aaggccgctc ctccccgaag cgaaccggcg cccccaccgc
    19561 gcgaaaagaa atgatccgcc ccgctggctg cccccggttc cggttctggt ggaagcgccg
    19621 ctttcccgct tcgcgccggg ccgccccgtc atgctgaggt tgtctggcat caccaaggcg
    19681 tacggcgggc gggtgttgtt cgccgatgcc acgctgcaag tcaatcgcca ggaccgcatc
    19741 gggctggtgg gcccgaacgg agccggcaag accaccctgt tctcgattat cctcgggcag
    19801 gagtccgccg acgccggcca gttcgactgc gagcgcggca tttcccttgg ccacctgccg
    19861 caggaaagcg ccccggtcgc cgatgaaacc gtcctcgaac tggccaccgc catcacctct
    19921 gaatacaccc ggctgcgccg catcctcaag gcctgggaag ccgatcaccc ggccgcggcg
    19981 ctggccccgg aggaaatcca tgacgacgcg cacgatcgct ttcgcgcgtt gggcggctac
    20041 cgcctggagg ctcgggccaa gcaaatcctc gccgggctgg gttttcgcca ggcggacttc
    20101 gaccgcccgg cgcgcgagat gagcggcggc tgggtgatgc ggacgcacct ggcgcggctg
    20161 ctgacgcagg agccggattt gctgctgctg gacgagccga ccaaccacct cgacctcgag
    20221 gcgctgctct ggttccagga atatctccag agttatccgg gcgcgatcct cgtcatttcg
    20281 cacgaccgcg agttcctcaa ccagatcgtc ggcagcattg tcgaaatccg ccagagccgg
    20341 ttgatccgct accggggcgg ctatgacgaa taccttgagc agcgcgaggc gtacgaacag
    20401 cagcaactgg ccgcctacaa acaccaggag cgcgagatcg cccgcctgct ggagttcgtc
    20461 aaccgcttcc gcgccaaaaa caccaaggcg gcccaggccc agtccaagct caagcaaatc
    20521 gagcgcatgg acaagatcgc cgcgcccgcg ggcgaggagc gcaagctcga gtttcaattc
    20581 ccgcagcccc gccgcggcgg gttgcgcgcc atcaaactcg aaggcatcca ccacgcttat
    20641 ggcgagaacg tcgtctatcg cggcctggac ttcgaggccg cgcgcggcca gcggatcgtg
    20701 ctggtggggc cgaacggcgc gggcaagtcc accctgctca agctccttgc cggcgtgatt
    20761 accccgcaag ccggcgcgcg cacccccggc cataacgtcc acgtcggcta ttattcccag
    20821 taccggatcg aacaactgcg cccggagcgc acggtgctgg aagaggcctt tgacactccc
    20881 cggagcctga ccgagcaatt cgtccgcacg ctgctcggct gcttcctctt cagcggcgac
    20941 gatgtgttca agcgggttga ggtgctcagc ggcggtgaaa agagccgcct ggccctggtg
    21001 aagctcctgc tggatccgcc caacctcctg ctgatggacg aaccgaccac gcacctggat
    21061 ttgtcgagcg tggatgcgct gacttacgcg ctcgaccagt tcgagggcac gctcatcttc
    21121 atcagccacg acgtctattt catccgcacg ctcgccaacc acgtcgtcca cgtcaacgcc
    21181 gggcgcctga cccattaccc cggcggctac cagtattatc tcgaccggac ccgggccgtt
    21241 tccgcccgcg cgggcctgac cgccgatggc gggccgggcg tttccgcgct tcccggccgg
    21301 ccggattcac ccgcgcgggc gtcctccccg gcggcggacc ggaaggagca gaagcgcgcc
    21361 gccgccgagc agcgccaggc gcgctaccgg gcgcgcaagg cgcaacagga gcgggtggac
    21421 cggctggaac aggagattca cgaactggag gcgcggctgg cggacctggt ggccgaactg
    21481 gagcagccgg gcacctacgc gaaaccgggc cgccccgcgg ccatcaaccg ggaattgagc
    21541 gaagcgcgcc gccgcctggc ggagcttaat cccgcgtggg aacgcgaagc cacgcggctg
    21601 gcggagctgg aatagcgcgg gctagggtga cgcggccggt tcgcgggtga tttggaggtc
    21661 caccaggcag tgggcaatcc ggacggcttg cggctggccg ggcggcggcg ccggcgggcg
    21721 gtcgttttgg aagcgcaggt aattgcggcc cggccgcgcc atcacgcgca ggtccagcgg
    21781 ctgggattcg cccggctcca gttgcgcgct ccagagcgtc tggccgcccg cgcaaagctc
    21841 cacccgccgg gcggaggccg ccgccaccca ggcgcgcacc cgaaagacgc aaggctgcgg
    21901 ccggtcattc acgaagtaga gcgaagtgac gcgcgcgcag gcccagcgcg ccgggcggga
    21961 gccgcggtcg cgcagcccgt agctgccgaa agaccagtgg gcccggtaaa caatctgcgc
    22021 cgcgtcgtcc gcaggcggcc gcgccgggtg tggcgcggga ttgagcagca cgcagacctg
    22081 ttccccggcg gcatccgcca cgaaccgcgt ccagccgcgc tgcgccaagt ccgcgagcat
    22141 cgccgccgcg ccgtcggtgt aggccttgcg gttgaagtag aggccggcaa agccgtaatg
    22201 ctcaagctgc cgggcgcacg gcgccaccgg cagctccgcc agggccgcct gccagtcttc
    22261 ccggggccgc ccctgcaccg agccgaagct gaaacgcagc cgctccgtcc acaagtacgg
    22321 gcggacgtgt tcgtaggaaa gcatgccgcc gatggggtct ccgtctatga agttcatcag
    22381 cggcaactgg aaaatcatcg ccccggccgg gagcgccgct tccagggcgc ggccaaaccg
    22441 ctggtcattc tccaccgcgc gggcgagcgc gggggtggcg tccttcgccg gcggcggcac
    22501 ctggtccagc aagccgagcc ccgccacccc ggcggccagg gcgtagctgg ccgcgcgggg
    22561 ccacgcccgc gcccaccggc tcatgcggga gaccaggaag aacaggacca gggccgagat
    22621 gaagatgctg tagcggttgg cgccccgaaa gtagagcagc ccgaacacca gccccagcag
    22681 gcagttcaac ccgcccaccg cggcgtacag cagcacccac aaacacagcg gcgcgtggct
    22741 gggcagccgc cggggcgtcg cgcgcaggtt cagcgcgcgc cgtccgaact cgaccgtcag
    22801 ccaccccagc gccaccaccc ccaccacccc caggtagggc gagaacatct cgccctgcac
    22861 ccacgccgcc gccgcatact cgcgcgccag gtcgctgagc cagctcaccc gatgctgcca
    22921 gggcggcagc accaactcga ggggcttgag cgcgtaaagc tccagctgct tgtagggccg
    22981 gattacggcg agctgattcg gcccatgcgc ccacccatac ccaatcgtat gcgcgttgac
    23041 ggccaggaat cccgcggcgg ccacggccag gaggattgcg cccgtggcca ggtccgcctt
    23101 gcggcgctcg agcaggtagc gcacccccag ccccaaaacc agaaactgcg cccacatgct
    23161 gaggttgtag ggattgccca tccccagcgc cagcgccgtc cccgcgctca gccagaaaac
    23221 ggcgccgccc cggcgcgcgc gccgccccgc gctcaaccac catacgctca gcaccgccag
    23281 cggcaccgtg taatcgtagc tcaacagcaa atgccccagc ccgcgcccgc tgtgatacca
    23341 ggtgaaggca aaaagcaacg cccccgccgc cgcccattcc cgccgccagc gcagcagccg
    23401 gcaggcggca tagaaggaag tggccgaggc cagaaaactg gccaggatgc ccaggttggc
    23461 cgcgaaaccc aggcaggtgt accgcgccac cagcccgagc aggaacgtcg tcaccggctc
    23521 atacatcggg taatcatccc agttggccgc atacggcgcc ccgagccggg gattgacccg
    23581 gctgaggaag ggcaggtaat caaactcgga ggcggcgcga atccaggtga gcatctggag
    23641 cgcgtcgccg gagtagcccg gaggcacgcg ccacgaagcg gggctcaggc gatcgttggc
    23701 cagcccccag accaccgtca cgaggatcca gatcgccccc aggtgcagaa ggcccgcccc
    23761 ggaaagccgc cagcagaagc gtcgtcgcac cccggcaatc taccggaacg aaacgcgggg
    23821 ctgaaggcta aagtcgggga aacccgccgc cgcgcggagc gccgcgccgc ccgcgcgccg
    23881 cgcccggccg ctcacacttc cttcacgatc ccccaatgca catccagtcg tttattcagg
    23941 tagagctgga ctgctttgag cagcgtttgg gcctcgagct tctggccggc ggccacaatc
    24001 tgccggagcg tcatcccggg gcgcacggca aaacaactct gcgcgatgat cggcccctca
    24061 tccaggttca tcgtcacaaa gtgggccgac acgccgatga tcttgacgcc ctgctcgtaa
    24121 gcctgccggt aggcctgcgg ccccgggaag ctgggcagca gcgaagggtg aatgttgatg
    24181 atcttgttct tgaaccgcca gacgaagttt gccgagagaa tcttcatgaa gcgcgccagc
    24241 acgacgaaat ccacctcgtg ctgcgccagc acccgcagcg cctgctgctc ggctttcgcc
    24301 cggtcctccc acgggagatg aacaaacggc agccgggcct tccgcgccag cggctccaaa
    24361 tcgcggcggt tggataagac cagcgccggc tcggccttgc gcagcttgcc ggcctggatc
    24421 gccgccagga gtttctccag gcagtgcggc tcccgcgtca ccatcagcgc catgcgctgg
    24481 cggcggcgcg gcgcggtaaa gcggacggtg atctccatct ccaactcgtc cgccagcacc
    24541 gccagcccgc gctccaccgc ccggcggtcc aggctgcggc ccggccacga ggtctggacg
    24601 gtcatgctga actgtccgcg cgtcacctgc tcttcgagcg cttcgatgtt gcccttctgc
    24661 tcgaaaagaa aatgcgtcac ccgggcgaca acgcccgtct tgtcccggcc aattacagtg
    24721 atggtggcta gcgctttcac cggcaacaaa aggaagaacc cgccgcgaaa tgtccagcta
    24781 atcttgcggg gtggccgcgc gcgcgccggt cccggcggac gctagcgggg ccggttgccg
    24841 gccggcgtgg cggctggcgg cgcggcggcc ggcggcgcgg ccctgggcgc gggcgtggac
    24901 agggcggcaa agccccgcgc caccaactcg gcggccctgg cgtcgcgcac cgtccgcgtg
    24961 gcgctgtcca gcaccaccac aatgacccgc cggccctgcc gcgccgcggt caccgcgatg
    25021 gaataccccg aagccgcaat atatcccgtt ttcaacccgt cgcagccctc cacctgcttc
    25081 aacagatggt tatgggtgcg cataatcacc gtcttgccgg gcacgttcgg gcgaaaaggc
    25141 cgctcgcgcg ccgaggtata acgcagcgta tcgcggcacc ggagcacctg gcggcacagg
    25201 atggacaggt cgcgcgccgt ggtgatgtca tgcttttgcc cccccgtcgg cggcaggcca
    25261 tgcacggagc tgaacaccgt gttgttcatg cccagctccc gggcccgccg attcatcagc
    25321 gcgacgaagg catccgtgct gccggcaacc ttctcggcca gcgccaccgc cgcatcattg
    25381 gccgattgga tcatgagcgc gtagagcaat tcgtccaccg taaacgactc cttctccgcc
    25441 agccacacct gcgagccgcc cgtctgcgcc gcctttgcac tcacgcgcac cgtatcggtc
    25501 agggaaagct gtttctgctc gatcttttcc agaatgatca gcaggtccat cagcttgagg
    25561 acgctggccg ggtagccttt cgcgtccgcc tggtcctcat acaacacctt gcccgtggcg
    25621 gcatccacga caatggcccc cagccaggga tcccggcaaa tgggtttggg gcgcgcggat
    25681 ttggccggcg ccggggccgc ggcagggcgg cgggcctttt gcgcggcagc cccggccgcg
    25741 ggcaggctgg ccgccagcaa caatgccaat cccgccccca gccacttcaa tgatttcgtc
    25801 gttgtcttca caataaaagt gctcgctagc ttccgcatag caggagcgcc ggtcaagccc
    25861 ccaagctccc gatttgcaga tttaccccct ccagcaccgt aagctgctga ttttacaggc
    25921 gaaagtcgtt tggggttcaa atgcgatgcc gttgagtttg ctaatcaggg ggattgctga
    25981 aactaccgtt tgggctgtaa gagccacccc ggccccgatg gaacgatata ccaccgtcca
    26041 gcagcccaac tgcatcgttt cggcttggga ttccgcaaaa acagaaagca tccggacacc
    26101 ccccctccct tgaaaacccc ctcccagcca gccttagccg gaacgattca gttgaacacg
    26161 cataattgac tggttttggg tgttca
//
LOCUS       DAOPQG010000048        25808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2419_length_25808_cov_15.056110, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000048 DAOPQG010000000
VERSION     DAOPQG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 25808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25808
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2419_length_25808_cov_15.056110"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            490..1299
                     /gene="thiD"
                     /locus_tag="PKI07_07240"
     CDS             490..1299
                     /gene="thiD"
                     /locus_tag="PKI07_07240"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOC50885.1"
                     /translation="MSKHRQLPVALTIAGSDSGGGAGIQTDLKTFAALGVHGTSAITC
                     ITAQNPEGVYGIQACRVEIVRRQIEAVFTALRPAAAKTGMLYSAALIRAVAEFFRRGP
                     RVPLVVDPVMVSTSGAALLKPAAVKALGAELLPLATLVTPNLDEAKILLGRKLDSVPD
                     LRDAARELHRRFGCAALVKGGHLRGLREAVDIFYDGRTELLLSAPFVRGVSTHGTGCT
                     YSAAIAGYLARGGSLPRAVRQAKDFITQAIAQSRTAAGHGVLNCFWRPAGG"
     gene            complement(1314..2795)
                     /locus_tag="PKI07_07245"
     CDS             complement(1314..2795)
                     /locus_tag="PKI07_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50886.1"
                     /translation="MIAKRFRTRSAAGAATLLLAASLGIPARAALTRPARITFDPAGV
                     LVVDGQKKFPLTLTIVPGPDARAPNGRPAYAEFADSGVLFMRSGGPAWNDDTISREHA
                     MQAAAAASGLRCCPWLGWDLSNFSPGDANREARLRTVIAAFKDSPGMGLWKGADEPDW
                     SNAHNPAQATPEQVAHVARLLRELDPAHPIWLVQAPRGTAASLRRYDAGWDVGGMDIY
                     PVSYPPGVHTEGKNKELSMVGDFTRLMKRVAGEKPFWMTLQIAFSGVVKPGRTLRFPT
                     FPEQRFMAYEAIINGARGLTYFGGGLPQTLNSRDQPLGFNWTYFDRVMRPLFAELGPR
                     SPLLPALLAPDAGLKLSVAPEAPPRRALARKEEEQNLPAPSPPRLVRDEAGADARAIE
                     CLVRQAGDDYYVLACKKEGPTVHVRFRGLPPEIHGAGAGAVLFEEPRKVDAQHGSFAD
                     WFGPFEVHVYHFAASRKAGRDGEAAPAGPEPLPSAPESRPPRL"
     gene            3044..3343
                     /locus_tag="PKI07_07250"
     CDS             3044..3343
                     /locus_tag="PKI07_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50887.1"
                     /translation="MRTLKTLTVTILAAACLALPAGWAANKQTAKPKPYPLTTCVVSG
                     QKLGGMGAPYVFTHGNREIKLCCKGCLKAFQKDPAKYLKKIEEAEAQAQKAPKAK"
     gene            3814..5199
                     /locus_tag="PKI07_07255"
     CDS             3814..5199
                     /locus_tag="PKI07_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC50888.1"
                     /translation="MKTFVLWLLLLLLAGCRGAPTRGEREARRQLQAVAGSYRPHDRK
                     PELPPLSGQSGLSNYLACALLNQPRVEAAYFDWRASVERITLARSLPDPQFTFQMDIQ
                     SVVTSVMPGLMGVVPWPGRPRLGAEVASAESAAKRAAFEAAALRSAFDLKRAYYQLHL
                     QTERLRVNRETLALWSQEERLARARNEAGQNTLAEILEIRAEQDRLRAAIANLEDSRA
                     AWMAQFKAALGLQAADPEPPLPERFESTPAGLTPDEWLATALARNQRLKAMAADVQAA
                     EAALQLARKDRLPDFSLGLMADVQMQPVLYRPAGTLSLPLWRDKIAARIAEAQAGKRA
                     AEARLTAGEIALAADFAGRAFLYREAERALALLKDQLLPQARQALAVTRAEYLAGQVD
                     FFRLLERTRALLSLELEQAEAATQREIMLAELSLIIQGMPPADRPPEDLAASRGPASS
                     ASMNTLTPNDL"
     gene            5206..6765
                     /locus_tag="PKI07_07260"
     CDS             5206..6765
                     /locus_tag="PKI07_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC50889.1"
                     /translation="MNSDPMPLQSPSPAPPLIPLLLLSAVLAAGTAGFLAGCGRGETA
                     GSPRSAAPGKTLYTCGMDPQVIQDHPGNCPICGMKLTPIKRPLGSDAAIRGSGERPNA
                     PGQPAPPPGEAHPSPGTAHEDREPGPAEEMDGAGATIAVDPVTSQNMGIRTALVTRGP
                     LRRTIRTVGTVDYDETSLAEVTPRVSGWVEKLYVNMTGQPVRRGEPLFEIYSPELYSA
                     QREYLLAREQPADAPGADSLRHSARTRLRLLDISDEQIDEIERAGEPRKTLRLVAPRD
                     GFVIEKAVVEGQRVAPGERLYRLADLARVWVLAQIYEQDLGCIQPGQEALVTLSSLPG
                     REFRGRVSYLYPGVDEKTRTAQVRLELPNPGGLLKPGMFATVQIVSELEPDALRIPNM
                     AILRSGTQNTVFVARDGGRFTPRTVTLGAAADDDTCQVLSGLSEGERIVTSGQFLLDS
                     ESQLREAIQKMLEPGAITPPAPADPVEYTCPMEEDAEVRKPGPGQCPKCGMNLVPAHT
                     VPHGRRPEPQP"
     gene            6781..10047
                     /locus_tag="PKI07_07265"
     CDS             6781..10047
                     /locus_tag="PKI07_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC50890.1"
                     /translation="MLQRIIDFSLRNKFLVLLGTAVLGVGGIYAVRHTRLDAIPDLSD
                     VQVIVYTEWAGQAPQIVQDQLTYPITTKMLSVPKAKVVRGYSFYGFSFVYVIFEDGTD
                     PYWARSRVLEYLSNLASLLPRGVQPALGPDATGVGWAFMYSLTSTNRDLAELRSIQDW
                     YLRYPLAAVEGVAEVASVGGFVKQYQVTVDPVRLRAYNLSVADVAAAVRRSNGEVGGR
                     ALEWAEKEFILRVRGYLQGLDDLRQVAVGVGERGVPILLGQVATVELGPDMRRGIAEL
                     NGKGEAVGGIVVVRHGANARQVIQDVKQKLDQAMRGLPPDIHCTIVYDRSVLIDRAVR
                     TLAVKLLEESLVVALVCLAFLMHLRSALVAIVILPLAVLMSFLVMFGQGISSNIMSLG
                     GIAIAIGAMVDAVIIMVENAHKHLERDQGRKPHWGIIRDAAVEVGPALFYSLLVITVS
                     FLPVFALQAQEGRLFKPLAYTKTYAMGAAAILSLTLAPILMGLFIRGRIPAEERNPLN
                     RLFIWLYHPVVDFVIKWRWPTILIAGAVIVWVFLPWNWLAARWLPEGPVREWAFRAGR
                     FFPYQNLGSEFMPPLYEGDLLYMPTTFPGLAPTKARELLQVTDKIIRQFPEVRHVFGK
                     AGRAETATDPAPLEMLETTILLKDEREWPRVDIRDENGRRIAHRRRTPDELVDALNAA
                     VQIPGLNNAWTMPIKTRIDMLATGIKTPVGIKVAGPDLAQLERIAAQVEAVVKTVPGT
                     RSAFAERTMSGNYIEFDIRRAAIARYGLTVGDVQEVLEVALGGLPLTTTIEGLERYTV
                     NLRYSRDFRENLDALRNVVVPTPAGPQVPLGQLADIRTVRAPMDIKSEGAVPNAWVYV
                     DVKDIDVGTYVRMTMRAVNEAAARGVITLPSGYHLIWSGQYEYLQRAQQRLRLVIPLT
                     LLIIILILYLNTRSVVKTAMVLLAVPFSLVGAIWILYLLHYHLSVAVWVGIIALAGLD
                     AETGVVMLLYLDLAYEDGKKRGLMNNLGHLRDAIYHGAVKRVRPKTMTACVIIAGLLP
                     ILWSQGAGADVMKRIATPMVGGVITSTVMELAIYPALYYLWRSRQLCHPPARAEAIPA
                     "
     gene            10272..11045
                     /locus_tag="PKI07_07270"
     CDS             10272..11045
                     /locus_tag="PKI07_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOC50891.1"
                     /translation="MQAQERRFGGIGRLYGRDGLERLRRSHVCVVGVGGVGSWAVEAL
                     ARSGIGELTLVDADKVCLSNVNRQLHALDENLGQPKVEVLARRVRAINPDCTVHPLPA
                     LFLKTNAGEILRAPYSFVLDAIDSPSRKCLLIARCHKQGIPVLTTGAAGGRRDPTAVQ
                     VMDLAFSSHDRLLQEVRRGLRVRHGFPRGARPFGVECVLSRERVFYGGKDSPSGQPPA
                     NKLAPGPGDGNYLGTASFVTGTFGFVAAARIIQRLTGAG"
     gene            complement(11065..11886)
                     /locus_tag="PKI07_07275"
     CDS             complement(11065..11886)
                     /locus_tag="PKI07_07275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOC50892.1"
                     /translation="MNYRLCDAHNHLQDDRLAPHRAAIWPALRRAGIAWMVVNGSCER
                     DWPQVLALARECPQVIPSFGCHPWYVKECSADWQATLLRHLEAVPSALGEAGLDRWIP
                     DPDLERQEAVFVWQLRLAAERNLPLSIHCLQAWGRLLEILRAEPRPRCGFLLHSYGGS
                     RELIAPLAELGAYFSLPGYFAHARKVRQRETFRHIPPERLLIETDAPDQLLPDELNRH
                     PLADVQTGKPLNHPANLGAVYEFAAGLLGEPVASLACRVEANFRRLFGPLAALPA"
     gene            12063..13406
                     /locus_tag="PKI07_07280"
     CDS             12063..13406
                     /locus_tag="PKI07_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC50893.1"
                     /translation="MQHAANSNASQYTRRSFLKSSTVMVAGLSVADFSLAAPAKETLA
                     VSGGAKTVTFPREELAALTKWPRYGEVEKKIVCDMLDSNRYYDVLPVFEKEWQEYTQS
                     PFVKNHINGTSALTSMYFALDLPPGSEVMVPSYTFFATCLALRFFNCVPIFVDIDPKT
                     ATWDVEDAKRKLTPRTRAVVPMHSWGLPCQMDLISDWAREKGLIVLEDAAHAHGASMM
                     GKKMGTWGMMGIFSFQASKVLPAIEGGMGMYQTREYYERAAAFGEYNDPIKFPPDSPV
                     RPYAGTGFGQKYRMHPLGAALARQQLKGLDQMNALVEKNVRAMNDRLTRLTGVTEPLC
                     RPEQKRVYYHRNMLLIDFKKLGFTRDALVKALKAEGVDVNFWDYPEQHKLKIYAEAKW
                     WHHPPTIPASMPGNAYINANHIFLPLIYGEAPELIAQYIQAFEKVWAQRANLASV"
     gene            13488..13991
                     /locus_tag="PKI07_07285"
     CDS             13488..13991
                     /locus_tag="PKI07_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOC50894.1"
                     /translation="MNKETSIKLLNRAVADELQAVHQYMYWHFHLDDQGFGPLASLLK
                     RIAIEEMNHVEQLAERILFLKGDVEMVPAGPVAKVTDAQKILAMAAEMEQAAITSYNQ
                     AAIECGQKADAVSKQVFEGLVNSEEKHFDQFDKQLDNIKRFGPNYLALQSFDRAPGQG
                     GSAEGSD"
     gene            14094..15179
                     /locus_tag="PKI07_07290"
     CDS             14094..15179
                     /locus_tag="PKI07_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC50895.1"
                     /translation="MMVMRHFRQWAMAILLGGALLGQAAAKPKAAPADTRRAAAPKKS
                     PAQAAPRSTPFKLTTREPGRRRVPGDFTIAVLPDTQQYTAAHRGGKPEMLVAQIEWVL
                     DNRVRRNIVYLTTEGDISNDGNKYPVQWHRATNAFFRLDDPKRTGLPDGLPWSAVVGN
                     HDTHHGGTILFNAFLGTNHFAGRSYYGGHYGTNNDNHYDLFHAGGQDFIVLALTMGAG
                     SNPKLMRWANAVLRAHAHRQAIVVTHSMLAPAAWPRPAPWTKEGPPIFHALTNNPNLF
                     LMLCGHRHGEGRRQEARGNGQYIHVLLSDYQRYTNGGNGFMRLLEFSPRRQIIRVKTF
                     SPWTGQWTTNPDGYFTLAWPQRRNVPR"
     gene            complement(15440..16471)
                     /gene="nadA"
                     /locus_tag="PKI07_07295"
     CDS             complement(15440..16471)
                     /gene="nadA"
                     /locus_tag="PKI07_07295"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOC50896.1"
                     /translation="MTELADVLAPGSDRFATRPALSRARRETLEREILALKQQLNAVI
                     LAHNYQVPEIQDIADYVGDSLGLSQQAAQTKADVIVFCGVHFMAETAKILSPDKTVVL
                     PDRNAGCSLEEACPADKLAALQKTNPNFYTVTYINCSAAVKALSDVICTSGNAAKIVN
                     AAPRDKDLLFVPDENLGQWVMEQTGRPMTLWRGNCYVHVEFRRESLLRARQAHPEAKV
                     VVHPESLREVRDLADAVCSTEKMIAYCRTSPAKQFIIVTESGIIHRLQKECPDKEFIP
                     APIFDPLRAPTDPCRCSECKYMKLNTLDKVRDCMKNLSPRIELPAEILQRAQLPIRRM
                     LEISARPGA"
     gene            complement(16565..18865)
                     /locus_tag="PKI07_07300"
     CDS             complement(16565..18865)
                     /locus_tag="PKI07_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOC50897.1"
                     /translation="MANLAPAAAGSGGGSRPRPAPETAPRECTLRAIILGTVLGMVFG
                     ASSLYLVLKVGLTVSASIPVAVISLALFRLWSKAGGRDATILENNIVQTAGSAGESIA
                     FGLGVTMPAILILGFDLEISRVMLVGLLGSLLGILMMIPLRRALIVAQHGQLKYPEGT
                     ACAQVLKAGAASPPVFAGKTPPQPASPAGGAIKATTIFTGFGIGLVYKTLMAAFKLWK
                     DVPEKIFGAPLKGGSLAAEISPELLGVGYIIGPRIASIMMAGGVLSCLVLTPLIMFFG
                     EGRETVLPPGTQPVGQMTPAQIRSAYLLYIGAGAVAAGGIISLLRSLPIILSSLRSGL
                     ADFKAAAAAHTATLRADRDLSMKTVSVGIAALILAILAAPSLHMNFLGAALIVIFGFL
                     FVTVSSRLTGEIGSSSNPISGMTVATLLLTCLVFLLVGWTGGTYYVTALSVGGIVCVA
                     ASNAGATSQDLKTGYLVGATPKYQQIAILIGAASSALLLGPILLGLNRTATVYVPAAQ
                     IAPGLRAPADARLGAAETLRGPQAQADANAYRTWHKTDPFGAPPGKYLVDASGLAVWL
                     VDPGINGAFKTRADGTEVRKFDAPKATLMSYIIKGMLDRKLPWGLVLFGVMIAFVLEL
                     CGVSSLAFAVGAYLPISSSAPIFVGGLVRWLLDRRLRRSARARQLDEARLAAESDQSP
                     GVLLASGYIAGGAIAGIVIAFVAGVLGRLDARITKWAEASNPFFGGPHADWLALIPFA
                     ALAGFLYLTGRRTRRLPLPHPPGGPG"
     gene            19000..22290
                     /locus_tag="PKI07_07305"
     CDS             19000..22290
                     /locus_tag="PKI07_07305"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOC50898.1"
                     /translation="MLKASARPAIRLRGVRHNNLKNVDLDLPLNQLVVITGLSGSGKS
                     SLAFDTLFAEGQRRYIETFSPYARQFFDRMDKPQVDSIEGIPPAIAIEQRNAVRTTRS
                     TVGTMTEICDHLKMLWPHLAQLHCRQCGRPVHQDSPAAIGRALLRDAAPDPGKTEVLV
                     TFDLPLTDKLPLADSLALIARQGYQRLLWQGQVVRLEDIPRAPRPAVLTVVQDRLRLA
                     ADNRARLIEACEQAYHFGKGKLAIREMDAAQNAIRTPRRFSNRRHCAECDLEYRDPTP
                     ALFSFNHPVGACPTCRGFGRIISIDYDLALPDRSRTLAQGVVKPWQTGLGAECQDDLL
                     RLCRKRGVPTDVPFHRLPRKWQKLVIDGDPKYGQDPAHEWPRAWYGVKGYFRWLESKA
                     YKMHVRVLLSRYRAYTPCPDCRGQRLQPEALLYRCRVPDSPPEASRPRETRNPPGAAP
                     PPPAPPAADGPPRSLTLADFYQLPIREALPFIEALAGRRLRQPHDPLALVLGEVRARL
                     SYLNEVGLGYLTLDRPTRTLSGGETERVSLTTCLGTRLVNTLFVLDEPSVGLHARDTD
                     RLVRLLEQLRAAGNTVVVVEHEARVIRAADQVIDLGPGHGAAGGEVVFQGAYPQLLRS
                     PRSLTGQYLSGRRRIELPPRRAVNGPPPAHRPGAGQAPPAPANASRRHPPASTRAEAG
                     PVLRLSRATRHNLKNLAVEIPLGRFVCVTGVSGSGKTTLVREVLLPALAARLQSTLPP
                     AKASDRLASEENRNAENHETEPRAPHSAILHGAEHLGRVALVDQSILGKTPRSNPAVY
                     IGAFDDIRAFFAQSEPAQQRGLNASAFSFNSPQGQCERCRGAGFEKIEMQFLSDVFIR
                     CPDCDGRRYRAHILAVKVGPKTTRARRPAPAAPRRSARSAGARSIADLLDATVEEAIA
                     FLGQFPESRPAQRAVESLKLLAEVGLGYLKLGQPINTLSGGESQRLKLVRHLAEVIVG
                     TATPGARPAPAAPPPPAARGPRPPAPRPTLFLFDEPTTGLHFDDVRVLLQVFQRLVDA
                     GHSVLVIEHNLDVIKSADWLIDLGPDAGDQGGRIVAVGTPEQVAACPRSHTGQALRPL
                     LAPQPPAG"
     gene            22385..23284
                     /locus_tag="PKI07_07310"
     CDS             22385..23284
                     /locus_tag="PKI07_07310"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOC50899.1"
                     /translation="MLTAMLGLEQLAELLEQNRPAARARVKEFTLGGRHFAFNSQPAI
                     MGVINLSADSWYRESVCLTAESAVRRGRVMHAQGADLVDVGAESTLAHAARVDGAGQN
                     SKLLPVIRALREAGILVSVETYEPAVTRACLEAGANLLNLTGTDRSDELFRLVAAHDA
                     AVIICYVQGRNVREVGDFNLEADPVTLMQDYFARQVETALRHGVDKILIDPGLGFYYR
                     NLQDSSVRVRHQMRVFLNTFRLRALGFPICHALPHAFEYFGEQVRCAEPFFAVLAALG
                     KTDLFRTHEVPRTKAVLDTLRVF"
     gene            complement(23389..24393)
                     /locus_tag="PKI07_07315"
     CDS             complement(23389..24393)
                     /locus_tag="PKI07_07315"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HOC50900.1"
                     /translation="MSAVVQVAVHPSQFPAQVRRDLLASLRARRVNHKFHYDSLRQTQ
                     QWLALHQVLSPSRTDPNCAAIYDQGFAAAVSGLNAGRVHLVGLGCGGGQKDTRLLQQL
                     RDAGRTVFYTPSDVSVAMVLVAWQAARAVLPAERCFPLVCDLGTADDLPAALAALPVG
                     AAARLITFFGMIPNFEPELILPRLGALARPGDGLLFSANLAPGADYDVGMRRILPQYD
                     NAPTRDWLMTFLLDLGVAADDGELRFGIEDRPLRHGLKRVAAYFHFRRPREIRVDEHR
                     VAFAAGDAIRLFFSYRHTPALVRELLEEEGLNVRGEWVAESGEEGVFLAGRPAGSAAG
                     "
     gene            24586..25263
                     /locus_tag="PKI07_07320"
     CDS             24586..25263
                     /locus_tag="PKI07_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC50901.1"
                     /translation="MVRLVLFDVDGTLIHTGGAGMKAFARTFAEEFRLADGFERLQFA
                     GRTDLSLVREFLGFHQIPATPQNLDRFFSRYVFWLDHLLRSSRTALCPGVWELLRGLQ
                     ALPQAPLLGLLTGNIRLGAEIKLRHFALWDVFQTGGFGDDHEERDHIAATVHQRARRI
                     LGHPLPGNEVVVIGDTPLDIRCARAIGARALAVATGGASLEELKRHQPDWAVPDLRAI
                     TARELAA"
     gene            complement(25265..25501)
                     /locus_tag="PKI07_07325"
     CDS             complement(25265..25501)
                     /locus_tag="PKI07_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC50902.1"
                     /translation="MPIYEFHCDKCGRDSEILVRSSRWKGTPCPHCGSTKLSKRLSVF
                     AASVASTPSCATPACDVSTSGACGGCCGGGPHRH"
BASE COUNT         4359 a         8936 c         8345 g         4168 t
ORIGIN      
        1 tccggcccca ggcctgggag caggccgcga agctggcgcg ggcgcgagcc tgggcaggag
       61 accgggcgtc gcggggcatg gttaacgccc ggcaacattg gccaaagggc gtcgagtaat
      121 agacataagc tccgaggcgt ccggagcggg gggaatcgag taatttcata agcgtgaaac
      181 ttccatagtt gtttgattgc caagccggga gagcggcccg gcggcccgcg cgcgcgggtc
      241 agttaaccgg gcagcggttt gtccccagcc accggaaaaa gggtatcagg ccggtggcgg
      301 cggtcaacga agatagcgat tggtctgccg caattcggcc gcgagggggg taattcgcgt
      361 ccaggatata tttcaaataa tagcccggtg aagtggcagg aggattgatt ggccgatctc
      421 cgcattgccg gacgattcag tccgcaggga cgggctttac gtggcgcggc ttcccgctac
      481 tcttgccgca tgtcgaaaca tcgtcaactc ccggtggcgc tcaccattgc cggctcggac
      541 agcggcggtg gggcgggcat ccagacggat ctgaagacct ttgccgcgct gggcgtgcat
      601 ggcaccagcg cgattacgtg catcacggcg caaaacccgg aaggggtgta tggcatccag
      661 gcgtgccgcg tggaaatcgt gcgccgccag atcgaagcgg tgttcacggc gctgcgcccg
      721 gcggccgcca agaccgggat gctgtattcg gcggcgctca tccgcgcggt ggcggagttt
      781 ttccggcgcg gcccgcgggt gccgttggtg gtggacccgg tgatggtatc cacgagcggc
      841 gcggccctgc tcaagccggc ggcggtgaag gccctgggcg cggagcttct gcccctggcc
      901 acgctggtga cgcccaacct ggacgaagcc aaaatcctgc tcggccggaa gctggattcc
      961 gtgccggacc tccgggacgc cgcccgggag ttgcatcggc gctttggctg cgcggccctg
     1021 gtcaagggcg gccacctgcg cgggcttcgg gaggcggtgg atattttcta cgacgggcgc
     1081 acggagctgc tgctgagcgc gccgttcgtc cgaggagtga gcacgcacgg cacgggctgc
     1141 acctattcgg cggcgatcgc cgggtacctg gcgcggggcg gctccctgcc gcgcgccgtg
     1201 cggcaggcga aggatttcat cacccaggcc atcgcgcaaa gccggacagc cgccgggcat
     1261 ggggtgttga attgcttctg gcgcccggcg ggcggctgat tcaacaaggt gggttaaagg
     1321 cgcggcgggc ggctttcggg ggccgacgga agcggttccg gccccgccgg agcggcctcc
     1381 ccgtcccggc cggctttccg gctggccgca aagtggtaca catgcacctc gaacgggccg
     1441 aaccagtcgg caaaactgcc gtgttgcgcg tccactttcc gcggctcctc gaagagcacg
     1501 gcgcccgcgc ccgcgccgtg gatttccggg ggcaggccgc ggaagcgcac gtggacggtg
     1561 gggccctctt tcttgcaggc cagcacgtag tagtcatcgc cggcttggcg caccaggcac
     1621 tcgatggcgc gggcatccgc cccggcctca tcccgcacga gccgcggcgg agacggcgcc
     1681 ggcagattct gttcctcctc cttgcgggcc agggcgcgcc ggggcggggc ttccggcgcg
     1741 accgagagct tgaggccggc gtcgggggcc agcaacgccg gaaggagcgg cgagcggggg
     1801 ccgagctccg cgaagagcgg gcgcatgacc cggtcgaagt acgtccagtt aaatcccagc
     1861 ggctgatctc gcgagttcag ggtctggggc aggccgccgc cgaaataggt taagccgcgg
     1921 gcgccgttga tgatcgcctc gtaggccata aagcgctgct ccgggaatgt tggaaaccgc
     1981 agcgttcggc cgggcttgac gacgccggag aaggcgattt gcagggtcat ccaaaagggc
     2041 ttttcgcccg ccacccgctt catcaggcgg gtgaagtcgc ccaccatgct caactctttg
     2101 ttttttccct ccgtatgcac gccgggcggg tagctcaccg ggtagatgtc catgccgccc
     2161 acatcccagc ccgcgtcgta gcgccggagc gaggccgcgg tcccgcgcgg cgcctgcacc
     2221 agccagatcg ggtgggcggg gtcgagttcg cgcaggaggc gggccacgtg cgcgacctgc
     2281 tccggggtgg cctgggcggg gttgtgcgcg ttgctccaat ccggctcgtc ggcccccttc
     2341 cacaacccca tgccggggga atccttgaac gcggcaatca ccgttcgcag ccgggcttcc
     2401 cggttcgcgt cgccggggct gaagttggac agatcccacc ccagccacgg gcagcagcgc
     2461 agtccgctgg ccgccgccgc cgcctgcatg gcgtgctcgc gcgagatcgt gtcgtcgttc
     2521 caggccgggc cgccggaccg cataaacagg acgccgctgt cggcaaactc cgcgtacgcc
     2581 ggccggccgt tgggcgcccg ggcgtccggc cccggcacaa tcgtcagggt gaggggaaac
     2641 ttcttttgcc cgtccaccac caataccccc gccgggtcaa aggtgatccg ggccgggcgc
     2701 gtcaacgccg cccgcgcggg gatgcccagg ctggctgcca gcagcagggt cgcggcaccc
     2761 gccgccgatc gtgtcctgaa tcgcttcgca atcatcgccc gcggtatcct tggcgggagc
     2821 gccgcccgcc cgtcaatgag aatgtcggag ccggcgcgcg aacggccgcc gccggggccg
     2881 gccgccacgg gcccgcgcgc cggggcgggc cgggccgggg ttcgccgccg ccggaggcgg
     2941 accggcgccc aagccgcccg gcccgcccgc cgacggggat tagaaattat ttttgagcaa
     3001 ttcagcccga cttgttgtat aatgagtgta atcattttaa gttatgcgta cattgaaaac
     3061 tctgactgtg acgattctgg cggcggcctg cctggcgctg ccggctggct gggcggcgaa
     3121 taagcaaacc gccaaaccca aaccctatcc gctgacgacc tgcgtcgtca gtgggcagaa
     3181 gctcggcggc atgggcgctc cctatgtttt cacgcacggc aaccgcgaga tcaagctgtg
     3241 ttgcaagggc tgccttaagg cgttccagaa agaccccgcc aagtacctca agaagattga
     3301 ggaagctgaa gcccaggccc agaaagcgcc gaaagccaag taaacccggc cgctggacgg
     3361 ccggctcacg cgaatgaagt tgctggccac actggtgttc tgcgccgcct tgatcggagg
     3421 gccttgcctc ccggcggcgt gcaccggggc ggtggcggcg gcttcggacg ggaatgcctg
     3481 cgcgtgcggt tcctgcggcg agaactgctg ctgccttgac ccgtcggctc ccgggcccgc
     3541 gtccgccccg gccgtccccg cgccatcacc cactccgttg gagcacctgc tcctgccgtt
     3601 cccggcggga acggcttttc tcccgccgcc cgcccttcat ttggcggttg ccccttcgtt
     3661 tttccctccc ggcccgactg cggccctccc tctgtatcag cggaactgcg cgttcctgat
     3721 ctgatccatt tcggttcccg cccgtagtgt gtacctcgcc ggcgcgtttg cgccgccgtt
     3781 cggggtgctg accgtcaccg atttccctta gttatgaaaa cctttgtcct ttggcttttg
     3841 ctcctgctgc tggccggctg ccggggcgcc cccacgcggg gcgaaagaga agcccggcgc
     3901 cagctccagg cggtcgccgg ttcttaccgg ccccatgacc gcaagcccga gctgccgccg
     3961 ctttccggcc aatccgggct cagcaattac ctggcctgcg cgctgctgaa ccagccgcgg
     4021 gtcgaggccg cttatttcga ctggcgggcg tcggtggagc gcatcaccct cgcgcgttcg
     4081 cttccggacc cgcaattcac cttccaaatg gacatccaga gcgtggtcac ctccgtgatg
     4141 ccgggcctga tgggcgtcgt gccctggccg ggccgcccgc gcctcggggc cgaagtggcc
     4201 tccgccgaaa gcgcggccaa gcgcgccgcg ttcgaggcgg ccgccctccg cagcgccttc
     4261 gacctcaagc gcgcctatta ccagctccat ttgcagaccg agcgcctccg cgtgaaccgg
     4321 gaaaccctgg ccctttggag ccaggaggag cgcctcgccc gcgcccggaa cgaggcgggc
     4381 cagaacacgc tggcggagat cctggaaatt cgggccgaac aggaccgcct gcgcgcggcc
     4441 atcgccaacc tcgaggactc acgcgccgcc tggatggctc aattcaaggc cgcgctcggc
     4501 ttgcaggccg ccgacccgga gcctcccctg ccggagcggt ttgaatccac gccggccggg
     4561 ctgacgccgg acgagtggct cgcgaccgcc ctggcccgaa accagcggct caaggcgatg
     4621 gccgccgacg tgcaggcggc ggaggccgca ctccaactcg cccgcaaaga tcggcttccc
     4681 gacttcagcc tggggctcat ggcggatgtg caaatgcagc cggttcttta ccggccggcg
     4741 ggaaccctgt ccctgccgct gtggcgcgac aaaatcgccg cccggatcgc cgaagcccag
     4801 gccggcaaac gcgcggcgga agcccgcctc accgccgggg agatcgcgct ggccgcggac
     4861 tttgccggac gcgccttcct ttaccgggaa gccgagcgcg ccctggctct cctgaaagac
     4921 caactcctgc cccaggcccg ccaggcgctg gcggtgaccc gcgcggaata tcttgccggc
     4981 caggttgatt tcttccgcct gctggagcgc acgcgcgcac tgctgagcct ggaactggag
     5041 caggcggagg cggccacgca acgggagatc atgcttgccg agctgtcgtt gattatccag
     5101 ggcatgccgc ccgctgaccg tccgccggag gaccttgccg cctcccgggg cccggcttcg
     5161 tccgcctcca tgaacaccct gacacccaat gacctttaac tttgtatgaa ctctgacccg
     5221 atgcccctgc aatcgccctc ccctgctccg ccgctcattc cgctgctctt gctgagcgcg
     5281 gtcctcgccg cgggcacggc cgggttcctg gctggttgcg gccggggcga aacggccggc
     5341 tcgccgcgct ccgccgctcc ggggaaaacc ctttacacct gcgggatgga tccccaggtc
     5401 attcaggatc atcccggcaa ctgcccgatt tgcggcatga aacttacccc catcaagcgc
     5461 ccgctcggga gcgacgcggc catccgcggt tcgggcgagc gcccgaacgc gccgggccag
     5521 cccgcgccgc cgcccggcga agctcacccc tctcccggca ccgcccatga ggacagggaa
     5581 ccggggccag cagaagaaat ggacggcgcg ggcgccacca tagcggttga tccggtcacc
     5641 agccagaata tgggcatccg caccgccctc gtcacccgcg gccccttgcg ccgcaccatc
     5701 cgcaccgtgg gcaccgttga ttatgacgag acttcgctgg cggaagtgac cccccgggtc
     5761 agcggctggg tggagaaact ttacgtcaat atgaccgggc agccggtgcg gcgcggcgag
     5821 cctttgtttg agatttattc gcccgagctt tacagcgccc agcgggaata cttgctggcc
     5881 cgggagcagc cggcggacgc gcccggggcc gattccctgc ggcacagcgc gcgcacccgg
     5941 ctgaggttgc tggacatttc cgacgagcag attgacgaaa ttgaacgcgc cggggagccg
     6001 cgcaagaccc ttcgccttgt ggcgccccgg gacggctttg tgattgagaa ggcggtggtg
     6061 gaaggccagc gggtggcgcc gggcgagcgg ctttatcggc tggcggacct ggcgcgggtc
     6121 tgggtgctgg cgcaaattta cgagcaggac ctgggctgca tccagccggg acaggaggcg
     6181 ctcgtcacgc tttccagcct gcccggccgt gagttccggg ggcgcgtgtc ctacctctac
     6241 cccggcgtgg acgagaaaac ccgcacggcg caggtgcgcc tggaacttcc gaatcccggc
     6301 ggcctgctca agccgggcat gttcgccacc gtccagatcg tctccgaact ggagccggac
     6361 gcgctgcgga tccccaacat ggccattctg cgcagcggca cgcaaaatac ggtcttcgtc
     6421 gcccgggacg gcggccgctt caccccgcgc acggtgacgc tgggcgcggc ggcggacgat
     6481 gacacctgcc aggtgttgag cggcttgagc gaaggcgagc gcatcgtcac gtccggccaa
     6541 ttcctgctgg attccgagag ccagctccgg gaggccatcc agaagatgct ggagccgggc
     6601 gcgattaccc cgcccgcgcc cgccgatccc gttgaataca cctgccccat ggaggaggac
     6661 gccgaggtcc ggaaacccgg cccgggccaa tgccccaagt gcggtatgaa cctcgtgccg
     6721 gcccataccg tgccccacgg ccggcgtcct gagccgcaac cctgatcccc gggagccacc
     6781 atgctgcagc gcattataga cttctcgctc cggaataagt tcctggtcct gctgggcacc
     6841 gccgtgctgg gggtgggcgg gatttacgcc gtccggcata cgcggctgga tgccattccc
     6901 gacctctcgg atgtgcaggt gatcgtttat accgaatggg ccgggcaggc gccccagatc
     6961 gtgcaggacc agctcaccta ccccatcacc accaagatgc tctccgtgcc gaaggccaag
     7021 gtggtgcggg ggtattcctt ctatgggttc tcgttcgtgt atgtgatttt cgaagacggg
     7081 acggatccct actgggcgcg cagccgcgtg ctcgaatatc tcagcaacct ggccagcctg
     7141 ctgccgcgcg gcgtccagcc cgcactcggg ccggatgcca ccggggtcgg ctgggccttc
     7201 atgtattcgc tcacctccac caaccgcgac ctggccgagt tgcgctcgat tcaggactgg
     7261 tatctgcgct atccgctggc tgcggtcgaa ggcgtggcgg aggtcgcctc ggtgggcggg
     7321 ttcgtcaagc aataccaggt cactgtggac ccggttcggc tgcgcgccta caacctgtcg
     7381 gtcgccgacg tggccgcggc cgtgcggcgc agcaacggcg aagtcggcgg ccgcgccctc
     7441 gaatgggcgg aaaaggagtt catactgcgc gtgcggggct atctccaggg actggacgac
     7501 ttgcggcagg tggcggtcgg ggtgggtgag cggggggtgc cgatcttgct cgggcaggtg
     7561 gccacggtgg agctggggcc cgacatgcgg cgcggcatag cggagttgaa cggcaagggc
     7621 gaagccgtcg gcggcattgt ggtcgtgcgc cacggcgcca atgcccgcca ggtcattcag
     7681 gacgtgaagc agaagctcga ccaggccatg cgcggcctgc cgccggacat tcactgcacg
     7741 atcgtttatg accgcagcgt gctgattgac cgggcggttc ggacgctggc ggtaaagctc
     7801 ctcgaagaaa gcctcgtcgt ggccctggtc tgcctggcct tcctgatgca cctgcgcagc
     7861 gccctggtgg cgattgtgat cctgcccctc gcggtcctga tgtccttcct ggtcatgttc
     7921 ggccagggca tcagttcgaa cattatgtcg ctgggcggca tcgccattgc cattggcgcc
     7981 atggtggacg ccgtcatcat catggtcgaa aacgcgcaca aacacctgga gcgcgaccag
     8041 ggccggaaac cgcattgggg gatcatccgg gacgccgcgg ttgaagtcgg cccggcgctt
     8101 ttctactccc tgctggtgat cacggtctcc ttcctgccgg ttttcgcgct gcaggcgcag
     8161 gaaggccgac tgttcaagcc gctggcctac accaagacct acgccatggg cgcggcggcc
     8221 atcctgtccc tcaccctggc cccgattctg atggggcttt tcatccgcgg ccgaatcccg
     8281 gccgaggagc ggaacccgct caaccgcctg ttcatctggc tctatcatcc cgtggttgat
     8341 ttcgtcatca aatggcgctg gccgacgatc ctgatcgcgg gggccgtcat cgtgtgggtt
     8401 tttctgccgt ggaactggct cgccgcgcga tggctgcccg aggggccggt ccgggaatgg
     8461 gccttccggg cggggcggtt ttttccttac cagaatctcg ggtcggagtt catgcccccg
     8521 ctctacgagg gcgaccttct ctacatgccg accactttcc cgggcctcgc tcccaccaag
     8581 gcgcgcgagt tgctccaggt cacggacaag attatccgcc agttccccga ggtgcgccac
     8641 gtcttcggca aggccggccg ggcggaaacc gccaccgacc ccgcgccgct ggaaatgctc
     8701 gaaaccacca ttctgctcaa ggatgagcgg gagtggcccc gggtggacat tcgggacgag
     8761 aacggccgcc gcatcgccca ccgccgccgc acgcccgacg agctggtgga cgccttgaac
     8821 gccgccgtgc agattccggg cttgaacaat gcctggacca tgcccatcaa gacccgcatt
     8881 gacatgctgg ccaccggcat caagacgccc gtcggcatca aggtggccgg gcccgacctg
     8941 gctcaactgg agcgaattgc cgcccaggtg gaggcggtcg tcaaaaccgt tccgggcacg
     9001 cgcagcgcct ttgccgagcg aaccatgagc ggcaactaca tcgagttcga tatccggcgc
     9061 gcggccatcg cccgctatgg cctgactgtc ggcgacgtgc aggaggtgct ggaggtggcc
     9121 ctgggcggcc tgccgctgac caccaccatc gaggggctgg aacgctatac ggtgaacctg
     9181 cgttacagcc gggatttccg cgagaacctc gatgcgctgc gcaacgtggt tgtgcccacg
     9241 cccgccgggc cacaggtgcc cctcgggcag ttggccgaca tccgaaccgt ccgcgctccg
     9301 atggacatca agagcgaagg cgccgtgccc aatgcctggg tttatgtgga tgtgaaagac
     9361 attgacgtcg gcacctacgt ccgaatgacc atgcgcgccg tcaacgaagc cgcggcgcgc
     9421 ggcgtcatta ccctgcccag cggctaccac ctgatctgga gtggccaata cgaatacctg
     9481 cagcgcgccc agcaacgcct gcggctggtg atccccctca ccctgctgat catcattctc
     9541 attctttacc tcaatactcg ttccgtggtc aaaaccgcca tggtgctgct ggccgtgccg
     9601 ttttccctgg tcggcgccat ctggattctg tacctgctcc actaccatct cagcgtcgcc
     9661 gtgtgggtcg gcattatcgc cctggccggt ctcgacgccg aaaccggcgt ggtcatgctt
     9721 ctgtatttgg acctggccta cgaggatggg aaaaagcgtg gcctgatgaa caaccttggc
     9781 cacctgcgcg atgccatcta ccacggcgcc gtcaagcggg tccgccccaa aacgatgacc
     9841 gcttgcgtga tcatcgccgg cctgctcccc atcctttgga gccagggcgc cggggcggat
     9901 gttatgaagc gcatcgccac ccccatggtt ggcggagtaa tcacctccac ggtcatggag
     9961 ctggccatct atcccgccct ctactatctc tggcggtcgc gccaactgtg tcacccgccc
    10021 gcccgtgcgg aagccattcc ggcctgaccg ttccgcccgc ctgcctgacg aagctgccag
    10081 ccggaatcgg gaagctcgca gcgcgccggc cagcccgccg cggggagtga cgacgccctg
    10141 aggttgctcc gcgcttccgg ggcgctttag gcgggtccag ggccgttgat aaacgaaggt
    10201 gcgcgcggca gcttgctttt ctccagacac ggcttggcgg gcgagggaga tttttgtagt
    10261 aatcagcggc aatgcaagcc caggaacggc gttttggcgg cattggccgc ttgtatgggc
    10321 gcgacgggtt ggagcgactc cggcgcagcc atgtgtgcgt ggtcggcgtg ggtggagtcg
    10381 gttcgtgggc ggtcgaggcc ctggcccgct cggggatcgg cgagttaacc ctggtggatg
    10441 cggacaaggt ctgcctcagc aacgtcaacc gccagcttca tgcactggat gaaaacctgg
    10501 gccagccgaa ggtggaagtg ctggcgcggc gcgtgcgggc cattaacccc gattgcacgg
    10561 tgcacccgct cccggcgctc ttcctcaaaa ccaatgccgg ggagatcctg cgggcgcctt
    10621 atagcttcgt gctggatgcc atagacagcc cgtcgcggaa atgcctgctc atcgcccggt
    10681 gccacaagca gggaatcccg gtgctgacaa ccggcgcggc gggcgggcgg cgcgacccga
    10741 cggcggtgca ggtgatggac ctggcgtttt cgagccacga tcgcctgctg caagaggtgc
    10801 ggcgggggct gcgggtgcgg catgggtttc cgcgcggagc gcgtccgttt ggcgtggagt
    10861 gtgtgctctc gcgggagcgg gtcttttacg ggggaaagga tagccccagt ggtcagccgc
    10921 ccgcaaacaa gctcgccccc gggccgggag acgggaatta cctcggcacg gcgagctttg
    10981 tcaccggcac gttcgggttt gtggcggcgg cgcgcataat ccagcggctg accggagcgg
    11041 gctgaccccg ccggagccgc gcgcttacgc cggcagagcg gccagcgggc cgaacaggcg
    11101 gcgaaagttg gcctcgacgc ggcaggccag cgacgccaca ggctcgccca ataacccggc
    11161 ggcaaactca tagacggccc cgagattagc cgggtggttg agcggtttgc cggtctgaac
    11221 atcggccagc ggatggcggt tgagttcgtc gggcaagagc tggtcggggg cgtcggtttc
    11281 aatcagcagc cgctccgggg ggatatgccg aaaggtttcg cgctgacgca ccttgcgcgc
    11341 gtgcgcaaag tagcccggca gcgagaaata agcgcccagc tccgccagcg gggcgatcag
    11401 ttcccgggag ccgccgtacg aatgcagcag aaacccgcag cgggggcgcg gttcggcgcg
    11461 caggatttcg agcagccggc cccaggcttg gagacaatgg atgctcaacg gcaggttgcg
    11521 ttccgccgcc agccgcaatt gccagacaaa caccgcttcc tgccgctcga gatccggatc
    11581 gggaatccag cgatccagcc ccgcctcccc cagggcggag gggacggctt cgagatgccg
    11641 caggagcgtc gcctgccaat cggcggagca ttccttcaca taccagggat ggcaaccgaa
    11701 ggaagggatg acctgcgggc attcgcgggc cagggccagc acttgcggcc agtcacgctc
    11761 acacgaaccg ttgaccacca tccaggcgat gcccgcgcgc cgcaaggccg gccaaatcgc
    11821 cgcgcggtga ggcgccagcc ggtcgtcctg caaatgatta tgcgcgtcgc aaagccggta
    11881 gttcatggcg tgtctccggc ggcgggcggg tgccggccgg gctttgccgc agttgcgcgc
    11941 ttgagccggc gatggcggtt cccgtgttga atatctcgct ttcgcaccgg gttaaacaaa
    12001 tgtcttgcat tatcctgccc gcggcgcagt attttgtcga aacctatagt caggatattc
    12061 ttatgcaaca cgcagcaaat tcaaatgctt ctcaatacac ccggcgcagc ttcctcaaat
    12121 ccagcacggt catggtggcc ggcttgtcgg tcgccgactt tagcctggcg gcgccagcca
    12181 aagagacgct ggcggtttcc ggtggggcca aaaccgtcac cttcccccgg gaggagctgg
    12241 cggcactcac caaatggccg cgctacggcg aagtcgagaa gaagatcgtc tgcgatatgc
    12301 tcgatagcaa ccggtattac gatgtgctgc cggtcttcga gaaagaatgg caggaataca
    12361 cccaatcgcc gttcgtgaag aaccacatca acggcaccag cgccctgacc agcatgtact
    12421 tcgcgctcga cctgccgccg ggcagcgagg tcatggtgcc ttcctacacc ttcttcgcca
    12481 cctgcctggc cctgcgcttc ttcaactgcg tgccgatttt tgtggatatt gaccccaaaa
    12541 ccgccacgtg ggacgtggaa gacgccaagc gcaagctgac cccgcgcacc cgcgcggtgg
    12601 tgcccatgca ttcgtgggga ttgccctgcc agatggactt gatcagcgac tgggcccggg
    12661 aaaaggggtt gattgtcctc gaagacgccg cccatgcgca cggcgcctcg atgatgggca
    12721 aaaagatggg cacctggggc atgatgggca tcttcagctt ccaggcgtcc aaagtcctgc
    12781 cggccattga aggcggcatg ggcatgtacc agacgcgcga gtattacgag cgcgccgccg
    12841 cctttggcga atataatgac ccgatcaagt tccccccgga cagccccgtg cgcccttatg
    12901 ccggcaccgg gtttggccaa aaataccgga tgcacccgct cggcgcggcc ttggcccgcc
    12961 agcaactaaa agggctggac cagatgaacg ccctggtgga aaagaacgtc cgggccatga
    13021 acgaccgcct gacccggctg acaggagtca ccgagccgct ttgccgcccc gagcaaaagc
    13081 gcgtgtatta ccaccgtaat atgttgttga tagacttcaa gaagctcggg ttcacacgcg
    13141 acgcgctggt caaggccctc aaggccgaag gcgtggacgt gaacttctgg gattaccccg
    13201 aacagcataa gctcaagatt tacgccgagg ccaagtggtg gcatcacccg cccaccattc
    13261 ccgcctccat gccgggcaac gcctacatca acgccaacca tatcttcctg ccgttaatct
    13321 acggcgaagc gccggagttg atcgcccagt acatccaggc tttcgaaaaa gtgtgggcgc
    13381 agcgcgccaa cctggcctcc gtgtagcgcc tttggaaccc tgacgccagg cgcaaccggt
    13441 tggtgctccc cggcaggccc gccggaacgt caggccattg actttatatg aacaaagaaa
    13501 caagcattaa acttctgaac cgggcggtgg cagacgaact ccaggcggtc caccagtaca
    13561 tgtattggca tttccacctg gatgaccagg gcttcggtcc gctggccagc ctgctgaaac
    13621 ggattgccat tgaggagatg aatcacgtcg agcaactcgc cgagcgcatt ctctttctca
    13681 agggcgatgt cgaaatggtg ccggccggac cggtcgcgaa ggtcacggac gctcaaaaga
    13741 ttctggccat ggccgccgaa atggagcagg ccgccatcac cagctacaac caggccgcga
    13801 ttgaatgcgg ccaaaaggcc gacgccgtct ccaaacaggt cttcgaggga ttggtgaaca
    13861 gcgaggaaaa gcacttcgac cagtttgaca agcagcttga taatatcaaa cgttttgggc
    13921 ccaactacct cgccctgcaa tccttcgacc gcgctcccgg gcagggcggc tccgcggaag
    13981 ggtcggatta agccactcta tcctcgacgc ggcccggccg ctcgctatcc ggtgccggcg
    14041 cggcgctgcc gttgcgccca accgctcggc ctttacgggc gactgagact tttatgatgg
    14101 tcatgcgaca tttccgccag tgggcgatgg ccatactgct tggcggcgcc ctgctgggcc
    14161 aggcggctgc caagccaaag gccgcgcccg cggacacccg ccgcgcggcg gcgccgaaga
    14221 aatccccggc gcaggcggct ccgcggtcca cgccctttaa gttgacaacc cgcgagccgg
    14281 gccggcggcg ggttccgggt gatttcacca ttgccgtgct gcccgatacg cagcaataca
    14341 ccgcggcgca ccggggcggc aaacccgaga tgctggtcgc gcaaatcgaa tgggttctcg
    14401 acaaccgcgt ccgccgcaat atcgtttacc tgactacgga gggagacatc agcaacgacg
    14461 gcaataaata tcccgtgcaa tggcaccgcg cgaccaatgc cttcttccgc ctggacgatc
    14521 cgaagcgcac gggactgccc gatgggctgc cctggagcgc ggtggtcggc aatcacgata
    14581 cccaccacgg aggaaccatc ctgttcaacg ccttcctggg caccaaccac ttcgcgggcc
    14641 gcagctatta cggcggccat tacggcacca acaatgacaa ccattacgac cttttccacg
    14701 ccggcggcca ggattttatc gtgctggctc tcacgatggg agcggggagc aaccccaaac
    14761 tgatgcgctg ggccaacgcg gtcctgcgcg cgcatgccca ccggcaggcc atcgtcgtga
    14821 cgcactcgat gcttgccccg gccgcctggc cgcgcccggc cccatggacc aaggaagggc
    14881 cgcctatctt tcacgccctg accaacaacc ccaacctgtt cctgatgctg tgcggccatc
    14941 gccatggcga aggccggcgc caggaagccc gcggcaatgg gcagtacata cacgtgctgc
    15001 tctccgacta ccagcgctac accaacggcg gtaacggctt catgcggttg ctggagtttt
    15061 caccgcgccg gcaaatcatc cgcgtgaaaa ccttctcgcc ctggaccggc cagtggacga
    15121 cgaaccccga cggttatttc acactcgcgt ggccccagcg gcgaaacgtt cctcggtagc
    15181 gtccctcacc tgatccctcc ggggaaacgc ccggcacacc caggcattga attcgcggag
    15241 tgaacctccg taatgaggaa ataaccgttc cccggccccg gatacgatcg ccccagcgcg
    15301 ggatcccttc agccccgatg cgatcgccat cgccaaaccg cagccgattc accggccttc
    15361 ctcggcgcgc aagagaccgg ttgccggggc gggtgccagc caaaacccgg cggcaaactg
    15421 cccgcccagt gccacaggct caagcccccg gccgggcgga gatttccagc atacgccgga
    15481 tgggcaactg ggcgcgctgg aggatttcgg cgggcaactc gatgcggggg gagaggtttt
    15541 tcatgcagtc gcgcaccttg tccagcgtgt tcagcttcat atatttgcat tcgctgcagc
    15601 ggcaggggtc cgtcggcgcc cgcaggggat cgaagatggg cgcgggaatg aattccttgt
    15661 ccggacattc cttctgaagc cggtggataa tgccggactc cgtcacgatg ataaactgct
    15721 tcgccgggct ggtgcggcaa taagcgatca ttttctcggt cgaacacacc gcgtcggcca
    15781 aatcgcgcac ctcgcgcagg ctttcgggat gcaccaccac cttcgcttcc ggatgcgcct
    15841 gccgggcgcg cagcaggctt tcccggcgga actcgacatg cacatagcag ttgccccgcc
    15901 aaagcgtcat cggccgcccg gtttgctcca ttacccactg gccaagattc tcgtccggca
    15961 cgaacagcag gtccttgtcc cggggcgcgg cgttgacgat tttcgccgcg ttgccgctgg
    16021 tgcagattac gtcgctcagc gccttgaccg cggcactgca gttaatataa gtgaccgtgt
    16081 agaaattggg attggtcttt tggagcgccg ccagcttgtc agccgggcag gcttcctcca
    16141 gggaacaacc cgcgttccgg tcgggcagca cgaccgtttt gtccgggctt aggatcttgg
    16201 ccgtctccgc catgaaatga acgccgcaga acacaatcac atccgctttg gtttgcgcgg
    16261 cttgctgtga aagcccgagc gagtcgccga cgtaatcagc gatatcctga atctcgggca
    16321 cctggtaatt atgggcgaga ataaccgcgt tgagttgttg tttcagcgcg agaatctcgc
    16381 gctccaacgt ctccctccgg gcgcgcgaca acgccgggcg cgttgcaaaa cggtccgagc
    16441 ccggagctaa aacatccgct aactctgtca tccagaacaa gctaacgcct cgctccgggg
    16501 gcgtcaaatc cagcccgcgg gagcgaggag ctttccccgc cgccaccttc ccgcgaacag
    16561 gttttcagcc gggcccgccc ggcggatgcg gaagcggcag ccgccgcgtc cggcggccgg
    16621 tcaggtacag aaagccggcc agggcggcaa acggaatcag cgccagccag tccgcatggg
    16681 ggccgccgaa aaagggattg ctggcctcgg cccatttcgt aatgcgcgca tcgaggcggc
    16741 ccaacacacc ggccacgaaa gcgatgacga tgcccgcaat cgcgccgccg gcaatgtagc
    16801 cgctggccag cagcacgccg gggctttggt cgctctccgc ggccagccgg gcttcatcga
    16861 gctggcgcgc gcgcgcggaa cgccgcaggc gccggtccag cagccagcgc accagccccc
    16921 cgacgaagat gggcgccgaa gaggaaatcg gcaggtaggc gccaacggca aaggccagcg
    16981 aggacactcc gcacaactcc agcacgaatg caatcatgac gccaaacaac accaggcccc
    17041 acggcagctt gcggtcgagc atgcccttga tgatatagct catgagcgtg gccttggggg
    17101 cgtcgaactt ccgcacttcc gtgccgtcgg cccgtgtctt gaaggccccg ttgatgcccg
    17161 ggtccaccag ccagacggcc aggccggaag cgtccaccag gtacttgccc ggcggagcgc
    17221 caaagggatc ggtcttatgc caggtgcgat aggcattggc atccgcctgc gcctgcggcc
    17281 cgcgcaaggt ttccgccgcg cccagccggg catcggccgg cgcccgcagc ccgggcgcga
    17341 tttgcgcggc tggcacatag accgtggcgg tccggttcaa ccccagcagt atggggccca
    17401 gcaacagcgc gctcgacgcc gcgccgatca ggatggcgat ctgctggtat ttgggcgtcg
    17461 cccccaccag atacccggtc ttcaaatcct gcgacgtggc gccggcattc gaggcggcga
    17521 cgcagacaat gccgccgacc gacagggccg tcacgtagta agtcccgccg gtccagccca
    17581 cgagcaaaaa caccaggcaa gtgagcagca gggtggcaac cgtcatgccg gagatggggt
    17641 tggacgaaga accaatctcg cccgtcagcc gggaggacac ggtgacgaac agaaagccga
    17701 atatcacgat cagcgccgcc cccaggaagt tcatgtgcag cgagggagcc gccaggatgg
    17761 cgagaatcag ggcggcgatg cccacgctga ccgtcttcat ggataaatcc cgatccgcgc
    17821 gcagggtcgc ggtgtgggcg gcggcggccg ccttgaaatc cgccaaaccg gaccgcaggc
    17881 tgctcaggat gatggggagg gagcgcagca ggctgataat gccgcccgcg gccacagccc
    17941 ccgccccgat gtacagcagg taggcgctgc ggatttgcgc cggcgtcatc tgcccgaccg
    18001 gctgggtgcc cggcggcagc acggtctccc gcccctcgcc gaagaacata ataagcgggg
    18061 tcagcaccag gcaactgagc acgccgccgg ccatcataat cgaggcaatc cgcgggccga
    18121 tgatgtagcc cacgcccagc agttcggggg agatttcggc ggccagcgaa ccgcccttca
    18181 gcggggcgcc gaagattttt tccggcacat ctttccagag cttaaaggcc gccatcaggg
    18241 tcttgtaaac cagcccgatg ccgaagccgg tgaaaatggt cgtggccttg atcgcgccgc
    18301 ccgccggcga agccggttgc ggcggcgttt tcccggcgaa aaccgggggc gaagccgccc
    18361 cggccttgag cacctgggcg cacgccgtgc cctcggggta cttgagctgg ccatgctgcg
    18421 cgacgatcag cgcgcgccgc agtggaatca tcatcagtat ccccagcagg ctgcccagca
    18481 gcccgaccag catgacccgg ctgatctcca ggtcgaagcc caggatcagg atcgccggca
    18541 tcgtgacccc cagcccgaag gcgatggact cgccggccga gccggcagtc tggacgatat
    18601 tgttctccag gatggtggcg tcgcggccgc cggccttgga ccagagccgg aaaagcgcca
    18661 gcgaaatcac cgccaccggg atggacgcgc tgacggtcag cccgaccttg agcacaaggt
    18721 agagcgacga agcgccaaaa accatgccca gcaccgtgcc gaggatgatc gcgcgcagcg
    18781 tgcattcccg cggcgcggtt tcgggcgcgg ggcgcggccg ggatccgcct cccgaaccgg
    18841 ccgctgctgg agccaggttc gccatgggtg gggattttcc gcgaaaaccg ccccgaagcc
    18901 agtgaaaagt gccggccccg tcccggtttc ccggcgcggc ggggtttttc ccgcggcgac
    18961 ttgccagccc gccccggcaa atctaccttg ctcccggcaa tgctgaaagc ttccgcgcgg
    19021 ccggcgatcc gtcttcgggg cgtccgccat aacaatctca agaacgtgga tctggacctg
    19081 ccgctcaacc agttggtggt gatcaccggc ttgagcggtt cgggaaagag ttccctggcg
    19141 ttcgatacgc tgtttgccga gggccagcgg cgctacatcg agaccttctc cccctacgcg
    19201 cggcagttct ttgaccgcat ggacaaaccg caggtggaca gcatcgaagg catccctccc
    19261 gccatcgcca tcgagcagcg caatgccgtg cgcaccacgc gctcgacggt tggcacgatg
    19321 accgagattt gcgaccacct caagatgctc tggccgcacc tggcgcaact gcactgccgc
    19381 caatgcggac gcccggtgca ccaggactcg cccgccgcca ttgggcgcgc cctgctgcgc
    19441 gacgcggcgc cggatcccgg gaaaaccgaa gtcctggtca ccttcgacct gccgctcacg
    19501 gacaagctgc cgctggcgga ttcgctcgct ctgattgccc ggcagggata tcagcgcctg
    19561 ttgtggcaag gccaggtcgt gcggctggaa gacatccccc gcgccccccg ccccgccgtc
    19621 ctgacggtgg tccaggatcg cctgcggctc gccgcggaca accgcgcgcg cctgattgag
    19681 gcctgcgaac aggcctacca cttcggcaaa ggcaagctgg caatccgcga gatggacgcc
    19741 gcgcaaaacg ccatccgcac cccgcgccgg ttctcgaatc gccgccactg cgcggagtgc
    19801 gacctcgaat accgcgatcc cacgcccgcc ctgttcagct ttaatcatcc cgtcggcgcc
    19861 tgcccgacct gccgcggctt tggccgcatc atcagcattg actacgacct ggcgctgccc
    19921 gaccgctcgc gaacgctggc gcaaggcgtg gtcaaaccct ggcagacggg gctgggcgca
    19981 gaatgccagg acgacctgct gcgcctgtgc cggaaacggg gcgtgcccac ggacgtgccc
    20041 ttccaccggc tgccgcggaa gtggcagaag ctggtgattg acggggatcc caaatacggc
    20101 caggacccgg cgcacgaatg gccccgcgcc tggtatggcg tcaaaggcta cttccgctgg
    20161 ctcgaatcca aggcttacaa aatgcatgtg cgcgtgctgc tctcccggta tcgcgcctac
    20221 accccctgcc cggattgccg cggccagcgg ctccagcccg aggcgctgct ctatcggtgc
    20281 cgcgtgccgg attccccgcc cgaagcctcc cgcccccggg aaacccggaa tccgccgggc
    20341 gccgccccgc ccccgcccgc accgcccgcg gcggacggac cgccgcgttc tctcacgctg
    20401 gccgattttt atcaacttcc catccgggaa gccctgccgt tcatcgaagc gctggccggg
    20461 cggcgcctcc ggcagcccca tgaccccctc gccctggtgc tgggcgaagt ccgcgcccgg
    20521 ctgagctacc tcaacgaggt ggggctgggc tacctgacgc tggaccgccc cacgcgcacg
    20581 ctttcgggcg gcgaaaccga gcgggtgagc ctgaccacct gcctgggcac gcgcctggtc
    20641 aacaccctgt ttgttctgga cgagccgagc gtcggattgc atgcgcgcga caccgaccgg
    20701 ctggtgcgcc tgctcgaaca actgcgcgcg gcgggcaaca cggtggtggt ggtcgaacac
    20761 gaagcccgcg tcatccgcgc cgccgaccag gtcattgacc tgggccccgg ccacggggcc
    20821 gccggcggcg aagtggtgtt tcaaggcgcc tacccgcaac ttctccgctc cccccggtcg
    20881 ctgaccggcc agtatctcag cggccgtcgg cgaattgaac taccgccgcg ccgggctgtc
    20941 aacgggccgc ctccggccca ccgccccggg gccggccagg ccccgcccgc tccggcaaat
    21001 gcctcccggc ggcatccccc ggcctccacc cgggccgagg ccggccccgt gctgcgcctt
    21061 tcccgcgcca cccggcacaa tctcaaaaac ctcgcggtgg aaatccccct ggggcgtttc
    21121 gtctgcgtca ccggggtcag cggttcgggc aagaccaccc tggtgcgcga ggttctcctg
    21181 ccggccctgg cggcgcgttt gcaatccacg ctgccgcccg ccaaagcctc ggaccggctc
    21241 gcgtccgaag agaaccgcaa cgcggaaaac cacgaaaccg agccccgcgc cccgcattca
    21301 gccattctcc acggcgccga gcacctgggc cgggtggcgc tggtggacca gtccattctc
    21361 ggcaaaactc cgcgctccaa cccggcggtc tatatcgggg ccttcgatga catccgggcg
    21421 ttcttcgccc aatcggagcc ggcgcaacag cgcgggctca atgccagcgc cttcagcttc
    21481 aactctcccc agggccagtg cgaacgctgc cgcggcgccg ggttcgagaa gatcgaaatg
    21541 caattcctga gcgacgtgtt catccgctgc cccgactgcg acggacgccg ctaccgcgcg
    21601 catatcctcg cggtaaaagt ggggccgaaa accacccgcg cccgccgccc ggccccggct
    21661 gcgccccgcc gctccgcgcg ctccgccggg gcccgctcca tcgccgacct gctcgacgcc
    21721 accgtcgagg aagccatcgc cttcctgggc cagttccccg aatcgcgccc ggcgcaacgc
    21781 gccgtcgaaa gcctgaagct gctggcggaa gtggggctgg gctacttgaa gctgggccag
    21841 ccgattaaca ccctgtccgg cggggaaagc cagcgcctga aactggtgcg ccacctggcg
    21901 gaggtcatcg tgggcaccgc cacgcccggc gcccggcccg ctcccgcagc cccgccgccg
    21961 cccgccgcgc gcggcccgcg ccccccggcg ccacgcccca ccctgttcct cttcgacgag
    22021 ccgaccaccg ggctgcactt cgatgacgtg cgcgtgctgc tgcaggtctt ccagcggctg
    22081 gtggatgccg gccattcggt gctcgtcatc gaacacaacc tcgacgtgat taaatccgcc
    22141 gactggctca tagacctcgg cccggacgcc ggggaccagg gcggccggat cgtggcggtg
    22201 ggcacgccgg agcaggtggc cgcctgcccg cgcagccaca ccggccaggc gctgcggccc
    22261 ctgctggcgc cgcaaccgcc cgccggctga cgccccctcc ccccgcccgc cgcgcgggcc
    22321 ggccgtgccg ccgccgcccc gcccgcccgg caagttccgg ctttcccgag cccggttttg
    22381 cggtatgctg acggccatgc tgggactgga acaactggcc gaactgctgg agcaaaatcg
    22441 tccggcggcc cgggcgcgcg tcaaagagtt cactctgggc ggccggcatt ttgccttcaa
    22501 ctcgcaaccc gccatcatgg gcgtgatcaa cctttcggcg gactcctggt accgggagag
    22561 cgtctgcctc accgcggaaa gcgcggtgcg ccggggccgg gtcatgcacg cgcagggagc
    22621 ggacctcgtg gacgtggggg cggaatccac cctggcccat gcggcccgcg tggacggcgc
    22681 ggggcagaac tccaaactgc tgccggtgat ccgggcgctg cgggaggcgg gcatcctggt
    22741 ttccgtggaa acctatgagc cggcggtgac gcgcgcctgc ctcgaagccg gcgccaacct
    22801 cctgaacctg accggcaccg accgcagcga cgagcttttc cgcctggtcg cggcgcacga
    22861 cgcggcggtg atcatctgct atgtgcaggg ccggaacgtg cgcgaggtgg gtgatttcaa
    22921 cctggaggcc gacccggtga cgctgatgca ggattacttc gcgcgccagg tggaaaccgc
    22981 gctccgccac ggcgtggaca agatcctcat tgacccgggg ctgggcttct actaccgcaa
    23041 cctgcaggac agcagcgtgc gggtgcgcca ccagatgcgc gtattcctga acaccttccg
    23101 gctgcgcgcg ctgggctttc cgatctgcca cgcgctgccc cacgccttcg aatacttcgg
    23161 cgagcaagtc cgctgcgccg agccgttctt cgccgtgctc gccgcgctgg gcaaaaccga
    23221 cttgttccgc acccacgaag tgccgcgcac caaagccgtg ctggatacgc tgcgggtttt
    23281 ctaacccggc cctttcccgg ccccgggcgg gagccgcgcc ggaaccgcgc gaacgccccg
    23341 ccgccagcaa ttccgccaac cggcggcggg cctgcccgcg ccgccaactc agcccgccgc
    23401 tgaacccgcc gggcggcccg ccaggaatac gccctcctcc ccggactcgg ccacccattc
    23461 gccccgcacg ttcagcccct cctcttccag cagctcgcgc accagcgccg gggtgtggcg
    23521 gtaggagaaa aagagccgga tggcgtctcc ggcggcaaac gcgacgcgat gctcgtccac
    23581 gcggatttcc cgcggccggc ggaagtgaaa ataggccgcc acccgtttca agccgtggcg
    23641 gagcggccga tcctcgatcc cgaagcgcag ctcgccatcg tccgccgcca cgcccaaatc
    23701 gagcaggaac gtcatcagcc agtcccgcgt cggcgcattg tcgtattgcg gcaggatgcg
    23761 ccgcatgccg acgtcgtaat cggcgccggg cgcgaggttg gcgctgaaga gcaggccatc
    23821 gccgggccgc gccagcgccc ccagccgcgg cagaatcagc tccggctcga agttcggaat
    23881 catgccgaaa aaggtgatca gccgcgcggc ggcgccgacg ggcagcgcgg ccagcgccgc
    23941 cggcaggtcg tccgccgtcc ccaggtcgca gaccagcgga aagcaacgct ccgccggcag
    24001 aaccgcccgc gccgcctgcc acgccaccag caccatcgcc acgctgacat ccgacggcgt
    24061 ataaaacacc gtccggcccg cgtcccgcag ttgctggagc agccgcgtgt ccttctggcc
    24121 cccgccgcac ccgagcccga ccaggtgcac gcggccggcg ttcaggccgg acaccgcggc
    24181 ggcgaaaccc tgatcgtaaa tggccgcgca attcgggtcc gtgcgggacg gcgaaagaac
    24241 ctggtgcagc gccagccatt gctgggtttg ccggaggctg tcgtaatgaa acttgtggtt
    24301 aacccggcgc gcgcgcagcg aggccagcag gtcacgccgc acctgcgccg gaaactggct
    24361 cggatgaacc gctacctgga ccaccgccga catggctcgg gattgctcac ggctccgcag
    24421 gctagtggaa cgccggacgc gccggcaagg ccggattgcg gcttcgcact ccggcggaaa
    24481 aaacgcgaac cgccccggcg gcggcggggg cggcgcgccg gcccgcccgg cacccgcggc
    24541 ggaagtcgtt gcccgaatcc gcgggaccgc gtaacctccc cgggcatggt gcgactggtt
    24601 ttgttcgatg ttgacggcac gctgatccac acgggcggcg cggggatgaa agcctttgcc
    24661 cggaccttcg ccgaggagtt tcgcctcgcg gacggcttcg agcgtttgca gttcgccggg
    24721 cgcaccgacc tgagcctggt gcgggagttc ctcggcttcc accagatccc ggccacgccc
    24781 caaaacctgg accgcttttt ctcccgctac gtcttctggc tggatcatct cctgcgctcg
    24841 agccgcaccg cgctctgccc gggggtgtgg gagttgctgc gcgggctgca ggcgctgccg
    24901 caggcgcccc tgctgggcct gctcaccggc aatatccggc tcggcgcgga aatcaagctg
    24961 cgccacttcg ccctctggga tgtgtttcag accggcggct tcggcgacga ccacgaagag
    25021 cgggaccaca ttgccgccac cgtgcatcag cgggcccgcc gcatcctcgg ccaccccctg
    25081 ccgggaaacg aggtggtggt gatcggggat acgccgctgg acatccgctg cgcgcgcgcc
    25141 atcggggcca gggccctcgc cgtcgccacc ggcggcgcca gcctggagga actaaaacgg
    25201 catcagcccg actgggccgt gccggatttg cgggccatca ccgcccgcga actggccgcc
    25261 tgactcaatg ccgatgcggg ccgccgccgc agcagccccc gcacgctccg ctggtgctga
    25321 catcgcacgc cggcgtcgcg caggacgggg tggacgccac cgacgccgca aataccgata
    25381 accgcttgga aagtttcgtc gagccgcagt gcgggcacgg ggtccccttc cagcggctcg
    25441 aacgaaccag aatctcgctg tctcgcccgc atttgtcgca atgaaactcg taaatcggca
    25501 taaatctaaa atagccgccg cccgcaaaag ctcaagtgcg gtttcccgcg caaggaacag
    25561 cgccctccca cggagcaatc tcccgcccct tgcgaagagg ctctgccccg caaaatttcg
    25621 ctgctccctc aaacccccgc cgccacactc gcaaaccttg cggattttat ccgcccctgc
    25681 gggacggcgc cgtttcgcgc ctccatcgcc cggcttcaat ccgcttttgg cagggtgaaa
    25741 tgccttttgc ctgatcgcta tcttcgttga ccgccgcctc cggcctgata ccctttttcc
    25801 ggtggctg
//
LOCUS       DAOPQG010000049        25335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2489_length_25335_cov_16.104470, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000049 DAOPQG010000000
VERSION     DAOPQG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 25335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25335
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2489_length_25335_cov_16.104470"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..1564
                     /locus_tag="PKI07_07330"
     CDS             422..1564
                     /locus_tag="PKI07_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOC50903.1"
                     /translation="MGGRRLVLLLAAAVVLAASGAIRAASAAPDPRSAFISFTEFSRF
                     TRSPGARTNEVILLSPEITAPIAWDELVLSWNVPPGVYLKAEARALYPERATRYYTLG
                     QWSDDPARFPRRSVPGQRDADGEVRMDTLALNHAARRVQLRLTVGGAAEPSGLKFLGV
                     SFCNTAAPIRKLAPNRAAWGKVLPVPERRQGEYEGGGGWCSPTSLSMVLAYWGERLQR
                     PELNYTVPETARAIADGPRADTGNWPFNTAFAGRHPGLRAYVTRLGDIAEMEDWIAAG
                     IPVILSVSSYLTRSPASGPDYGHLIVCVGFTDTGDVVANNPGVSVRKNVRARQVYARE
                     QVVRNWARSKNTVYLIYPETAAIPANRLGHWDDGSAAAGAVEGKGQ"
     gene            1664..2680
                     /locus_tag="PKI07_07335"
     CDS             1664..2680
                     /locus_tag="PKI07_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50904.1"
                     /translation="MKISKPLHVFLGLWLAVLAGRAPAAEGVVYQGSGGPGRGKHIVF
                     LAGDEEYRSEEGLPMLAQILAARHGFKCTVLFAINPADGTIDPLTLTNMPGMAALDAA
                     DLCVMGLRFRELPDAQMKHFVDYLNAGRPIIALRTSTHAFKYEVNKASPYAKYDWHST
                     VWPGGFGRQVLGETWVAHHGNHGRESTRGVIHEAFKGHPVLRGVADLWGPTDVYAITQ
                     LPADAQVLVWGQVLAGMKPEDPPVAGPKNSPMMPLVWIRNYTGENGRTAKVLTTTMGA
                     AVDLENEGLRRLLINAAYWAVGLEKKIPRRANAACVGEYQPSWFGYGKFKPGRKPRDL
                     AWPH"
     gene            3010..4056
                     /locus_tag="PKI07_07340"
     CDS             3010..4056
                     /locus_tag="PKI07_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOC50905.1"
                     /translation="MKRAIALGASIMLGLGAGYGQAPPVLNMQLSNGVVRLQMTGSVG
                     AAYRIERTAGFGRAGGWQVLTNLAVLAGSPCVVIDKDGGQPGCFYRAQVVVSPSASNM
                     VWIPAGSFVMGSPASEALRFADETQHTVTLSKGFYMGKYPVTQREYLKVMGKNPSFFT
                     GDLDRPVEEVSWKDATNYCGRLTQQERAAGRLAAGWGFRLPTESEWEYACRAGTTTAF
                     HYGNALRQGMANFFTYNEYDAAIGNISTNSSGVGYQGRPVAGGSYPPNAWGLYDMHGN
                     VLEWCRDWYGAYPKGSVVDPQGPASGMNRAMRGGYWFSDAAYCRAAYRHCNGYPGLRS
                     GCTGFRVVLSSGQP"
     gene            4281..4382
                     /locus_tag="PKI07_07345"
     CDS             4281..4382
                     /locus_tag="PKI07_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOC50906.1"
                     /translation="MGSLKKRRKAKINKHKRRKKLRAHRHKKRTWQK"
     gene            4461..6158
                     /locus_tag="PKI07_07350"
     CDS             4461..6158
                     /locus_tag="PKI07_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC50907.1"
                     /translation="MRRRFWRAGGWFVIGCWLAAGCGSVPESGSPAPGKRSANAARSA
                     KAAARSFERAVEAHARYAAAVIQEMNGETQLAEEGYYQAALQDPGDDTLTLAVSERFL
                     RRQQTQRALEVMLRAAEQPDASGRVYARLGEIYVRLDKPAEAEAASRVAIRKAPGTIA
                     GYRNLVQLCMARQQPEAALKVLDEAARQTGVGADFLVTLAEMYAGVGLKAAALKDEAN
                     AAARGLLNRAAQTGALTPLLRLKLADGYLLLGDAERAAEFYLEVLKRPPDLPLVQERV
                     RANLTSIYLRGSDHPRASEQLRAILRDDPTNPQAYYYLGRLALEDRQPAEAVEAFSKA
                     ILLSPDIESAYYYLAVAQMEVAKPGEALATLEKARQRFAQSFAMEFYTALAYNRQKDY
                     AQAVRHFIVAEVIAKATDPGHLNEGFYFQLGAACERNGDYAQAEKYFQQCLQLAPDFA
                     EALNYLGYMWAERGEKLPAARELIERAVKAEPRNAAFLDSLAWVLFKLGQPGEALPHA
                     LRAVELSEQPDATLFDHLGDIYHALREPDKARAAWRQSLAVEPSAEVRQKLEANTPP"
     gene            6201..6584
                     /locus_tag="PKI07_07355"
     CDS             6201..6584
                     /locus_tag="PKI07_07355"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HOC50908.1"
                     /translation="MPYRFKKHYTREEARELLPLIRQWLQQMAQRRAELEKVERRLGG
                     LMAPGSDLGGGLVNTWVRTLVALNELLLEFARREIQLKSLDRGLVDFPAIIDGREVFL
                     CWEQDEEDVEFWHELDGGYAGRERV"
     gene            complement(6611..8125)
                     /locus_tag="PKI07_07360"
     CDS             complement(6611..8125)
                     /locus_tag="PKI07_07360"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOC50909.1"
                     /translation="MSPFVQIELLPLGKVLRVKRGTPLPDALFAQGVEFPCGGRGRCR
                     GCKIKVLAGSLPITAEEKRKLSPAELDAGWRLACRGRAEGDLKIELAQWEAAILTDHS
                     ALDFTPQPGWGLAVDLGTTTLVAQLVDRQTGSVLGVRTALNAQAQHGADIMSRIEFAL
                     DGSHQGVLEHLTRQQIGALAADLLAERAADARPLESVIVVGNTAMHHLFCGLSLEPLA
                     LYPFEPASPGRQQFDAAQLGWDLPGNPPVQFLPCLGGFVGSDILAGLLATRLPQSERL
                     AALIDLGTNGEIVVGNRERLLCASTAAGPAFEGARIAMGMRAATGAIAGVRVQNGRLQ
                     CRVLGNAAPRGICGSGLVDAVAAGLSLGWIRPNGRLANGDSLPLAGPVALNQRDVREL
                     QLAKGAIAAGLRILLEQWGATKADLSQVFLAGAFGNYINHASARRIGLLDFPADQVRP
                     AGNSALLGAKLALFDPPGHDHDYAAIRAKIRHVSLNEDERFQEIFAEEMLFPPD"
     gene            8300..8396
                     /gene="ffs"
                     /locus_tag="PKI07_07365"
     ncRNA           8300..8396
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKI07_07365"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8490..10286
                     /gene="dnaX"
                     /locus_tag="PKI07_07370"
     CDS             8490..10286
                     /gene="dnaX"
                     /locus_tag="PKI07_07370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOC50910.1"
                     /translation="MSYQVIARKYRPQRFRDVVGQEHVTQTLAHAIEQKRIAHAYLFC
                     GPRGTGKTTIARIFAKCLNCEDGPKVEFEEKDQRCQEIAEGRSLDVFEIDGASNNGVE
                     QVRELRETCKYAPASSRFKIYIIDEVHMLSTGAFNALLKTLEEPPAHVKFMFATTDPE
                     KVLPTILSRCQRFDLRRIPTALIAQHLAHIADLEKVQVAPAALHAIARGAEGAMRDAE
                     SALDQLISFCGGRIEEADVLSMFGLTAQGQLLELTRMVLAGEVETALRLLNDLANHGK
                     DLRRLLSDLLNHFRNLLIFQVSRGELDMLEVSEAEAAELKEQSPMAGADALTRIMEVF
                     AEAERQLRDAASKKILVEVALLRAIEARAAVRVDTLLQQLQSLRGPAETRPAPAGAAP
                     PSHFAPEPSASAGSPKPAAGGKRRAPDAAAEAEPAGTAAAAEELPGVAPGSPRRTGDL
                     GGLWSGLLEAVGRASQFIRSYLLGAHPVAFAENVLTIGYDPEFGDHLGLVDTPRTHAL
                     LQTKLAELGHPNVQIKFIQTEALASPALPPDTASPPPAVSATARRTAVKPAASAANKP
                     EAVPFSQEDFKNDPLIQKALEIFKGRIVEVRA"
     gene            10337..10651
                     /locus_tag="PKI07_07375"
     CDS             10337..10651
                     /locus_tag="PKI07_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOC50911.1"
                     /translation="MSSIGKLMKQAARMQQQMEQIQAALAARTVEATSGGGAVKVVAK
                     CDGTIAAVRIDPQALNPEDAQVLEDMILTATNQALAQAREISNTEMGKATAGFNLPGL
                     TS"
     gene            10711..11316
                     /gene="recR"
                     /locus_tag="PKI07_07380"
     CDS             10711..11316
                     /gene="recR"
                     /locus_tag="PKI07_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOC50912.1"
                     /translation="MAALPEPIALLVDALSRLPGIGPRSAERIALHLAQAETDAVGKL
                     AEAILQARARVRFCEVCGALTELSPCAICADPRRDGSLVCVVERPVDILSLEKARTFR
                     GRYHVLGGRISPLSGVEPEDLRIAELETRLVHGQVQEIILALGTDVEGEATGFYLAQR
                     LARPGLRITRVAYGLPAGTGLEFADELTLSHALEGRREMPG"
     gene            11357..11974
                     /locus_tag="PKI07_07385"
     CDS             11357..11974
                     /locus_tag="PKI07_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOC50913.1"
                     /translation="MPAPAAVVFDLGKVLVDFDYHIAARRIAARSRMSLVEIAQFINQ
                     SSLFYQYESGALTTRQFFEEICQATGFRGSQAEFGASFADIFVEIEPMIRLQAELRQR
                     GIPTYVFSNTNELAIEHIRQRFPFYAHFDGYILSYEQGAMKPDARIYEVVERQAGRRG
                     ADLLYLDDRPENIAAGAARGWQVILQESPEKSRAAVRKLGLLNPD"
     gene            12020..12868
                     /locus_tag="PKI07_07390"
     CDS             12020..12868
                     /locus_tag="PKI07_07390"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOC50914.1"
                     /translation="MRSQRQAMDWSLVLISQGIEVTIDPGDEGAGWGLLVPRQDCARA
                     LQTLRQYRRENRAWPWQQPVFRPGLLFDWGSLAWVLLIGAFYGLNSRGDLQAVGVMDG
                     AAVAQGQWWRLFTAIWLHADLAHLATNAALGLVLLGLAMGHYGTGTGLLAACLAGAGG
                     NLLAGLLSLQTHRSLGASGMVMGSLGLLAVQSFSLWRTSTRAVKLVVSGVCGGIMLFV
                     LLALTPGTDIVAHLGGFLGGLVLGVLLSLAPKMAHKLYANLACGVAFAILVIVPWWLA
                     LWGAGR"
     gene            13133..14011
                     /locus_tag="PKI07_07395"
     CDS             13133..14011
                     /locus_tag="PKI07_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC50915.1"
                     /translation="MEPEADDQVSARDRERSSYDGDTAIKLYLREIGQVKLLTPEEEI
                     QLAARIKRRDKKAREQMIKANLRLVVKIARDYEGIGLPLLDLISEGNIGLMKAVERFD
                     PAKGGKLSTYGAWWIKQSIKRALANQSKTIRLPVHLVDKISRMRRTSLRLQEELGREP
                     TDEELGEELGISAARVSQMRMAAVRPASLDAPIGDEDSNNFSEVVEDENADTPYQQLE
                     DKTVTRMLREMIKTLDHREATILRARFGLDGGPQKTLEEVGAKFGVTRERVRQIQNIA
                     LKKLRKMIEKMEAIKA"
     gene            14055..14591
                     /locus_tag="PKI07_07400"
     CDS             14055..14591
                     /locus_tag="PKI07_07400"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOC50916.1"
                     /translation="MSLTRPTPADLEKAIRNIPDFPKPGIQFKDITPVLADARLFSGS
                     IDLLTERFRPGDVDAVVGIDARGFIFAAAAAVKLQAGLVPVRKQGKLPYRTHEQQYNL
                     EYGTATVAVHVDALKPGSRVLLIDDLLATGGTAAAAAALVRRLEARILEISFLIELQF
                     LGGRQKLQDYPVRSLIVY"
     gene            14607..16844
                     /locus_tag="PKI07_07405"
     CDS             14607..16844
                     /locus_tag="PKI07_07405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC50917.1"
                     /translation="MHWLQTLDLGLFRFVNLKLANPIFDLVMPFASGNRFFAPVLLLA
                     LVLLAWKGRTRGALCVLMLVLIVLLGDGFICKPLKRTLLRPRPYAALAEVRRPTREFS
                     FSTPVETAALRLGDRASLPSAHAANWFAGTMVLFIYYRRSVWFMLPVAVLVSFSRIYN
                     GSHYPSDVLVGAILGSGYAAAGVWSLNALWQRAGRKWFPLWWRAMPCLLEPERRASDA
                     PVPDSGARSLAAAAGAPHATVDQHWLRLGYLWIAISLLARWAYLASGIIQLSEDEAYQ
                     WVWSKHLALSYYSKPPLIAYTQFLGTWLWGDTAFGVRFFSPVIAAMIGVILLRFFARE
                     VNARAGFFLLLIVSATLLLSAGAVLMTIDPLSVLFWTAAMLAGWRAVQEDASTRPWLW
                     VGLWMGLGFLSKYTELFQWLCWAVFFALWPAARRHLRRPGPWLALLINLLCALPVLIW
                     NHQHEWITVRHLAECAGADRPWHPTLRYLGEFLGAELGLLNPVFFGATVWAAIAFWRR
                     GRHDPRLVFFFSMGAPLFAAYLLHSFRSRVLPNWIAPAVLPLFCFMVIYWDTQWRLGR
                     ARIKSWLAVGLGLGFAAVLLGHNTDLVKVLTGQRLPVSLDSLRRVREWDQVARVVGAA
                     RQELLAEGKPVFIIGNHYGMVGQLSFYLPEARAAVTKEPLVFFRASPIPKNQFYFWPG
                     YENRQGQNAIFVRELKRDQENTPPAPPDLQRAFESVTPLGVRNVLYGGRVARRLQLFA
                     CRDLR"
     gene            17070..18041
                     /locus_tag="PKI07_07410"
     CDS             17070..18041
                     /locus_tag="PKI07_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOC50918.1"
                     /translation="MFAAFLAAGLSSAMADTIQLKENAAITGRILAEKPDQIAVDVGY
                     TVLVIPRNQVAKVLRSDAPASAAKPRRGARGGEGSGSVETDEARPGFYSAAGKPAAIR
                     TVLELATQLGGAVVQIRTPGGLGSGFFINEDGFLITNFHVIEGESQISVEVYHQQGGQ
                     LDRQTYKQVRIIALNKFADLALLKVEDKDAPRFRFVPLGNSDALSVGERVFAIGSPLG
                     LERTVTEGILSTKTREVGGLLYLQTTAQINPGNSGGPLFNLGGEVIGVINMKVTAGEG
                     LGFAIPVESLKLFLDHRDAFAYSNDNPSNPYRYLEPPGRLRQNPVEE"
     gene            18268..20094
                     /locus_tag="PKI07_07415"
     CDS             18268..20094
                     /locus_tag="PKI07_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50919.1"
                     /translation="MKPLRDKFTARWQEEVIKPLERELNVSLDTYPGLLQGQLTLAVV
                     RNTPPDEDHSPFGYLLLLDTRDKAGVLRTNLAHLRKQWAAAGKTLKTEPIRGLEFSVL
                     SVSTNEMPPTLSKWLWHAPVFPPLITYPELKQGPVSPSRKDDFVLDLLVELLTANPDL
                     VLGQVDSLLVAGNSVRNVERVVARLTGGTAPVLGDAIAYQTSQQAHFRDAPCYGWVNV
                     KALADSLSRAAAESAAAEPPDPLEPLKPDQVISATGLANCQTLAFSFRDSNAGSLIHC
                     FLTVPDSARRGLFPALAGPPKDASPPPFVPSDVALFFRWRLSLPGAWTSFEQMLKDLS
                     PPAFSAIQLILDTANARAQQDEPGFNLKQALLVNLGDDLISYERAPRSGSPAPQTTPS
                     LWLLGSPNPELLAVALKRLFVIFPQGDAFAEREFLGRKIYSVPAPPMPFATAAVSRPA
                     PPRTLNWGASAGYVALSTDIALLEEYLRADQNQIKPLRDKPGLLEAAQNVGGMGTGLF
                     RYENQAVTMRAVFEAAKNDLQAATNGFGPSLFPALPGITGPESNLSFWMDFSLLPPFD
                     RIAQYFPFSLYTFGANVEGFTLKLFIPASPSAGDSAPAQPAN"
     gene            complement(20099..20962)
                     /locus_tag="PKI07_07420"
     CDS             complement(20099..20962)
                     /locus_tag="PKI07_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50920.1"
                     /translation="MKRITSIAFCAALLTACASYGKDAATASSSRMALAAVPMAELPA
                     KAADLVLRAKPADRKATTLDVVKSALGINPTAATSIVGAIAHAVPDMAAVAAAAAAVE
                     QPRQASAIAKAAAAAAPAQASAIVAAVCRVAPAYYPNVAVAVSQVAPEANREIVQAVG
                     AAQPTLQPALEKVLAGYQGKIASVGGVLAEAGMAAQNPGQTGPAAVTPPSAPSPVAPS
                     APSTPIASVTPVVTPEVPATPTPLPELPVPPARGPAVGPPYFPLITTPTNVPPSGSTE
                     LPTGGRNYAKP"
     gene            complement(21103..22791)
                     /locus_tag="PKI07_07425"
     CDS             complement(21103..22791)
                     /locus_tag="PKI07_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOC50921.1"
                     /translation="MKPSQMEKKPVSISPAGEEFPLPLPEEYPAEFARLKKLAARHRQ
                     AGREIVVVVGLGFVGAVMAAVVADATDKKGRPTKFVIGMQRPSTRSFWKIRLLNRGVS
                     PVKAEDPEVEQIIARCVNEKQTLVATYTEEALKLADVVVVDVQCDYLKESLGNVRTGS
                     AEMAALEASMATIAENIPPAALVLIETTVAPGTTEQIAYPIMKKIFTKRGIRSEPLLA
                     HSYERVMPGRNYVASIRDFWRVCSGVNPAARKRVTRFLNEVLHTDRYPLTVLDRPIES
                     ETAKIVENSYRATILAFLDEWSLFAERNGVDLKKVIEAIKVRPTHSNIIFPGPGIGGY
                     CLPKDGGLGVWAYRHIFGFEDDIFKITPLAIDINDTRSLHAPQLVRDALRNMGKPIAA
                     AEVLLLGAAYREDVGDTRYSGSEIIVRKLSEMGAEVKVHDPYLDHWWEFEAQDTYPRP
                     NYSWARFFHRQAPLKNLRLEKDLWGALKGIDALVLAVRHDQYLKLDPDRVVKSAGQPF
                     AVVDCFCVLDDEKIRRYLELGCEVKGMGRGHIKRIKDSLNLQPSRGKAKAKTGR"
     gene            complement(22860..23942)
                     /locus_tag="PKI07_07430"
     CDS             complement(22860..23942)
                     /locus_tag="PKI07_07430"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOC50922.1"
                     /translation="MRERIGILTGGGDCPGLNTVIRAVVKCATKHGWETIGFLEGFEG
                     LLEPVRYRRLDYKEMDALLFVGGTILGTSNKGRFAAKTGHGETHRIPRDILRRAKANI
                     QQLGLRALVVVGGDGSLTTAQQLFEQGVPIIGVPKTIDNDLAATALTFGFDSAVACAT
                     DALDRLRTTAQSHDRVMVLEVMGRYAGWIAITAGISGGGDVILIPEIPFHLERVCAKI
                     IEREREGKRFTLVVVAEGAREQGADFVTAGGAEASREARLGGIGAVVAAGIQKRTGKE
                     TRVCVLGHLQRGGAPTSFDRQLCTRFGTRAVQLIAEEKYGHMVALRPPDTIAVPITEA
                     IGRLRLVPLEGDLVQTARALGISLGD"
     gene            complement(24113..>25335)
                     /locus_tag="PKI07_07435"
     CDS             complement(24113..>25335)
                     /locus_tag="PKI07_07435"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC50923.1"
                     /translation="VATNNPQSYQWRRNGENIAGATEATLTVNNAQEADAGAYTVQVS
                     NGVGPVVSAAAVLTVLGMPPEITLQPASQTNQAGSTVSFTVAATGQAPLAYRWQKDGV
                     DLSDDGIISGAQTDTLTLAGVTAAEEGSYAVGVTNAAGGVRSEAAVLVVQNPPVITLN
                     PAGQAVAAGATVTFSVEATGTALSYQWKLNGDPIAGATASACTRNNVQTADAGAYTVT
                     VANAVGSVTSDPAVLTVNNPPILAAIPDRTIHVGSTVVISSSVTHPDLDQTLTFSLEP
                     GAPAEATLDTATGVFRWTAPETSLNTTNPVTIRVSDDGTPSLGDARSFSITVMARPAI
                     LSAVLAGDFINLTWSSIPGLTYRVQYKLNLADAEWTDLMDVTAADWTTVATDSVIAYE
                     DPIPQRFYRIEVQD"
BASE COUNT         4165 a         7964 c         8522 g         4684 t
ORIGIN      
        1 acggcgcgcc cgcgctgggg cggcggcggc cgaactggtg gtccgggttt ttcccatccc
       61 gcacggaata cccacggacc ggagcgcggg cattttgccc gcttaccggc ttcgaacggg
      121 ctaagcgggc aggatgcccg cgcgccggcc tcgggcgtga atcgggcgat ccggttgctg
      181 gcagtttagg agagacatga gattggatgt tgagaatgaa atggcgtcaa gggtccgcgg
      241 tgggcttagg gtaaacggcg cgcccgcgct ggggcggcgg cggccgaact ggtggaccgg
      301 gtttttcccc tcccgcatga aatattcaca gaccggagcg cgggcatttt gcccgcttac
      361 aggcttcgaa cgggctaagc gggcaggatg cccgcgcgcc gggcttgcag gtgcgaaatg
      421 tttgggcggg cgccgcctcg tcctgctgct ggcagcggcg gtggtgctgg cggcctcggg
      481 cgcgatccgg gcggccagcg ccgcgcccga tccacgcagc gcgtttatca gctttaccga
      541 gttttcccgg ttcacccgaa gcccgggggc gcggacgaac gaggtgattc tgctttcccc
      601 tgaaatcacg gccccgattg catgggacga gctggtgctt tcctggaatg tgccgcccgg
      661 ggtgtatctc aaagcggagg cgcgcgcgct ctaccccgag cgcgccacgc gctattacac
      721 gctggggcag tggtcggacg atcccgcccg gtttccgcgg cggagcgtgc cggggcagcg
      781 ggatgcggat ggcgaggtgc ggatggacac gctcgccctg aaccacgcgg cgcgccgggt
      841 gcagcttcgc cttaccgtgg gcggggcggc ggagccgtcc gggctgaagt ttctcggggt
      901 gtccttttgc aacaccgcgg cgccgattcg gaaactggca cccaaccggg cggcctgggg
      961 caaggtgctg ccggttccgg agcggcggca gggcgagtat gagggcggcg gcggctggtg
     1021 cagccccact tcgctttcga tggtgctggc gtattggggc gagcggttgc aacggccgga
     1081 gttgaactac accgtgcccg agacggcccg ggcgatcgcc gacgggccgc gcgccgacac
     1141 ggggaactgg ccgttcaaca cggcgtttgc cgggcggcat cccgggctgc gggcctacgt
     1201 gacccgcctg ggggatattg ccgagatgga agattggatt gcggcgggca tcccggtcat
     1261 cctttcggta tcctcctatc tcacgcgcag cccggccagc gggccggact acggccacct
     1321 gattgtctgc gtggggttca ccgacaccgg cgacgtggtg gcgaataatc ccggggtgtc
     1381 cgtgcggaag aatgtccgcg cccggcaggt ttatgcgcgg gagcaagtgg tccgcaactg
     1441 ggcgcgttcc aagaatacgg tttacctgat ttacccggag acggcggcga ttcccgccaa
     1501 ccggctgggg cattgggatg acggaagcgc ggcggccggc gcggtggagg ggaagggcca
     1561 ataggcggct tgcggaatcc gggccggcgc gcgccgttgg gcccggcaaa agagggcgcg
     1621 gctgggtgct tgtcatgggg cgcggcgcgg ggcagacttg ggcatgaaga ttagcaagcc
     1681 gttgcatgta tttctggggt tgtggctggc ggtgctcgcc gggagggcgc cggccgcgga
     1741 gggggtggtt taccagggct cgggcgggcc ggggcggggg aagcacatcg ttttcttggc
     1801 gggggacgag gaatatcgct cggaggaggg gttgccgatg ctggcgcaga tcctggcggc
     1861 gcggcatggg ttcaaatgca cggtgttgtt tgccatcaac ccggccgacg ggacgattga
     1921 tcccctgacg ctgaccaata tgccggggat ggcggcgctg gacgcggccg atttatgcgt
     1981 gatggggctg cggttccggg aactgccgga tgcgcagatg aagcattttg tggactacct
     2041 gaacgcgggc cggccgatca tcgccctgcg caccagcacg catgcgttca aatacgaggt
     2101 gaataaggcc agtccgtatg cgaaatacga ctggcacagc accgtttggc cgggcgggtt
     2161 tggccggcaa gtgctggggg agacgtgggt cgcgcatcac gggaatcatg gccgggaaag
     2221 cacgcgcggg gtgattcatg aagcgttcaa ggggcacccg gttttgcgcg gcgtggcgga
     2281 cttgtggggg ccgacggatg tttatgccat cacgcagttg cccgccgatg cccaggtgct
     2341 ggtttggggg caggttttgg cgggcatgaa gccggaggat ccgccggtgg cggggcctaa
     2401 aaacagcccc atgatgccgc tggtctggat tcggaactac acgggagaga acggccggac
     2461 ggcgaaagtg ttgacgacga ctatgggcgc cgcggtggac ctcgaaaacg agggcctccg
     2521 gcggctgctg atcaacgccg cctactgggc ggtggggctg gagaagaaga ttccccggcg
     2581 cgccaacgcc gcttgcgtgg gcgaatacca gcccagttgg ttcggctacg gcaagttcaa
     2641 gccgggacgg aaaccccggg atttggcctg gccgcattaa taaggaaccg cccgctgcgt
     2701 gaggggccgc gatttgccca cgcctcgcgc ggcttctgaa ccctccccga ggccgcagct
     2761 ttcctggcgg ccggctattc gcagcgctcc tttggacgga acgaagaggt tcggggactc
     2821 ggttggacta tctggctggg gcgtgcctga ttttgggtca gcgagtctcc ggttggagcg
     2881 ggttgccgct ccgggctgcc ggagaggcct gggcgggggc agtttccgcc gccgccgggg
     2941 aaccggcacg cgatgtgctg atggaggggc gggtcgccga tggcgggcta tggatttgaa
     3001 gagaaaacta tgaagagagc aatcgcgctg ggcgcgagta taatgctggg gttgggggcg
     3061 ggctatggcc aggctccgcc cgtgctgaat atgcagttgt ccaacggggt cgtccggctg
     3121 caaatgacgg gctcggtggg agccgcttac cggattgaac ggacggccgg ctttggccgc
     3181 gcgggcggct ggcaggtgct gacgaacctg gctgtgctgg cgggcagtcc ctgcgtggtc
     3241 attgacaagg atggcgggca gcccgggtgt ttttatcgcg cccaggtggt ggtcagcccc
     3301 agcgcctcga acatggtctg gataccggcg ggcagctttg ttatgggcag cccggcgagc
     3361 gaagcgctgc ggtttgcgga tgagacgcag cataccgtga cgttgagcaa ggggttttat
     3421 atggggaaat acccggtgac gcagcgcgag tatctgaagg tgatgggcaa gaacccgagt
     3481 tttttcacgg gagacctgga tcgtccggtg gaggaggtga gttggaagga tgcgacgaac
     3541 tactgcgggc ggttgacgca gcaggagcgg gcggcggggc ggttagcggc gggctgggga
     3601 tttcgattgc cgacggagtc ggagtgggag tatgcgtgcc gggcggggac cacgacggca
     3661 tttcactacg ggaatgcctt acgccaggga atggccaatt tcttcaccta taacgaatat
     3721 gatgccgcga tcggaaacat cagcaccaac agctccgggg tgggctatca agggcggccg
     3781 gtggcgggag gcagttatcc gccgaatgcg tgggggctgt atgacatgca cgggaacgtg
     3841 ctggaatggt gccgggattg gtatggggca tatcccaagg ggagcgtggt ggatccgcag
     3901 gggccggctt cgggcatgaa ccgcgccatg cgcggcggct actggttctc tgatgccgct
     3961 tattgccggg ccgcctaccg ccattgcaat gggtatccgg gcctccggag cggttgcacg
     4021 ggttttcggg tggtcctgtc ttccggccag ccgtagccgg cgcggcgggg ggtggctttg
     4081 cggcggaagc ccgtttccgg gtgttggcgg cgtttctttc cttcggcctg caagcaggct
     4141 tgaccttggg gcagccatcg cgcacggtat gcaggcaaac aaaccggctg gggttgatga
     4201 acggttgcgg ggcggtgccg gcaccgcgcg ccgcctgcgg accaccaacc gggcgaagca
     4261 cgatacagag acgactgcct atgggctcat tgaagaaacg acggaaagct aagattaaca
     4321 agcataaacg gcgcaagaag ctgcgcgcgc atcgtcacaa gaagcgcacc tggcagaagt
     4381 agccgggcgg ccttagccgg ttgcccaatc agcattcggc gccacccgtg ctcggagcgg
     4441 ggcgggtggc gctttattgc atgcggcgtc gtttctggag agccgggggg tggttcgtca
     4501 tcggctgctg gctggcggcg gggtgtggct ccgtgcccga gtccgggagc ccggcgccgg
     4561 ggaagcgttc cgctaatgcc gcccggtcgg ccaaagcggc ggcgcggtcc tttgaaagag
     4621 cggtcgaggc gcatgcccgt tatgcggccg cggtgatcca ggagatgaac ggggagacgc
     4681 agcttgcgga ggagggttat taccaggcgg cgctgcagga cccgggcgat gacacgctca
     4741 ccttggcggt gtccgagcgt ttcctgcgcc gccagcagac ccagcgggcg ctggaggtca
     4801 tgctgcgcgc cgcggagcag ccggatgcct cggggcgggt ctatgcgcgg ctgggggaga
     4861 tttatgtccg cctggacaag ccggcggagg ccgaagccgc cagccgggtg gcgatccgga
     4921 aagcgccggg gacgatcgcc ggttatcgga atcttgttca gctttgcatg gcccggcagc
     4981 agccggaggc ggcgcttaaa gtgttggacg aggcggcccg gcagaccggg gtgggcgcgg
     5041 atttcctggt gaccctggcc gagatgtatg cgggggttgg cttgaaggcg gccgcgctca
     5101 aggacgaggc aaatgcggcg gcgcgcggct tgttgaaccg ggcggcgcaa accggggcgc
     5161 tgaccccgct gctgcggctg aagctggcgg acggctacct tctgctgggc gacgcggagc
     5221 gggcggcgga gttttacctc gaggtgctga agcggccgcc ggatctgccg ctggtgcagg
     5281 agcgggtgcg ggccaatctc acttcgattt acctgcgcgg cagcgaccac ccgcgggcca
     5341 gtgaacagct ccgggccatc ctgcgcgacg acccgaccaa tccccaggcc tattactacc
     5401 tgggccggct ggcgctggaa gaccgccagc cggcggaagc cgtcgaggcg ttcagcaaag
     5461 ccatcctgct cagcccggac attgagtcgg cgtattacta cctggccgtg gcccagatgg
     5521 aggtggcgaa acccggcgag gccctggcga ccctggagaa ggcgcggcag cggtttgccc
     5581 agagtttcgc gatggagttc tataccgcgc tggcctataa ccggcagaag gattacgcgc
     5641 aggcggtgcg gcattttatc gtcgccgaag tcatcgccaa ggccaccgac ccggggcatt
     5701 tgaacgaggg gttttacttt cagttgggcg ccgcctgcga acggaacggg gattacgcgc
     5761 aggcggagaa gtattttcaa caatgcctgc aactggcgcc cgatttcgcc gaggccctga
     5821 actacctggg ctacatgtgg gcggagcgcg gcgagaaatt gccggcggcg cgggaattga
     5881 tcgagcgggc ggtcaaggcg gagccgcgga acgcagcttt cctggacagc ctggcgtggg
     5941 tgctgttcaa gctcggccag cccggcgagg cgctgccgca cgcgctccgg gcggtggagc
     6001 tgtcggagca gccggacgcc accctctttg atcacctggg cgacatctac cacgcgttgc
     6061 gggagccgga caaagcgcgc gcggcctggc ggcagtcgct ggcggtcgag ccgagcgcgg
     6121 aggtgcgcca aaaactggag gccaacacac ccccatgagc ggaaaaaccc atcgcctatc
     6181 cggcccgccg gcctgttgtc atgccctacc ggtttaagaa acattacacg cgggaggaag
     6241 cccgggagct gctgccgctc atccggcagt ggctccagca aatggcgcag cgacgcgccg
     6301 agctggaaaa ggtcgagcgg cggttgggcg ggttaatggc gccggggagc gacctgggcg
     6361 gcgggctggt gaacacctgg gtgagaaccc tggtggccct gaacgaactg ctgctggagt
     6421 tcgcgcgccg ggaaatccag ttgaagagcc tggatcgcgg gctggtggat ttcccggcga
     6481 ttattgacgg gcgcgaggtg ttcttatgct gggagcagga tgaggaggac gtcgagttct
     6541 ggcacgaact ggacgggggc tatgccgggc gcgaacgggt atagcgtagg ccgcggagtg
     6601 gcgggcggga tcagtccggc gggaagagca tttcttccgc gaagatttcc tggaaccgct
     6661 catcctcgtt cagggagacg tgccggattt tggcccggat cgcggcatag tcgtggtcgt
     6721 gcccgggcgg gtcgaataag gccagcttgg cgcccagcag ggcggaattg ccggccggac
     6781 ggacctggtc ggcggggaaa tccagcaggc cgattcgccg cgcgctggcg tgattgatgt
     6841 aattgccgaa ggcgccggcc aggaagacct gggagaggtc ggccttcgtc gcgccccatt
     6901 gttccaggag gatgcgcagg ccggcggcga tggcgccctt ggccagttgc agttctcgca
     6961 cgtcccgttg attaagcgcg accggccccg ccagcggcag cgaatcgccg ttggccagcc
     7021 gtccgttggg ccggatccag cccagggaca gcccggcggc cactgcatcc acgaggccgc
     7081 tgccgcaaat cccgcgcggg gcggcattgc cgagcacccg gcattgcagc cggccgttct
     7141 gcacgcgcac tccggcaatg gcgcccgttg ccgcccgcat gcccatggcg atgcgcgcgc
     7201 cctcgaaggc cgggccggcc gccgtggacg cgcacagcag acgctcgcgg ttgcccacga
     7261 cgatttcgcc gttggttccc aggtcaatca gcgccgccag gcgttcgctt tgaggcaggc
     7321 gggttgccag caggccggcg agaatgtcgc tgcccacgaa cccgccgagg cagggcagga
     7381 actgcaccgg agggttgccg ggcaaatccc agcctagttg ggcggcgtcg aactgctgcc
     7441 gccccggaga agccggctcg aagggataca gcgccagcgg ttcgaggctg aggccgcaga
     7501 acaggtggtg catggctgtg ttgccgacga cgatcacgct ttcgagcggg cgcgcgtcag
     7561 cggcgcgttc cgccaggagg tcggccgcca gggcgccgat ctgctggcgc gtcaggtgct
     7621 ccagcacgcc ctggtgcgaa ccgtccaggg cgaattcaat gcggctcatg atgtcggcgc
     7681 cgtgctgggc ctgcgcgttg agcgccgtgc gcacgcccag cacgcttccg gtctgccggt
     7741 ccaccaactg cgccacgagg gtggtggtgc ccaggtccac cgcgagaccc cagcccggct
     7801 gcggcgtgaa atccagcgct gaatgatcgg tgagaatcgc cgcttcccat tgggccagct
     7861 cgattttcag gtctccctcc gcccgcccgc ggcaggccag ccgccagccg gcgtccagct
     7921 cggcggggga aagtttccgc ttttcctccg cggtaatcgg cagcgagccg gccagcactt
     7981 taattttgca cccccggcag cgcccgcgcc cgccgcacgg aaactcgact ccctgcgcaa
     8041 aaagcgcgtc cggcaagggg gtgccgcgtt ttacccgcag caccttgccc agcggcaaca
     8101 actcgatctg gacgaacggg ctcatggcgt ccggttagct tgtccgcatt atactcatgc
     8161 gcgcaatacg ctaaccgcaa tcccgcccgg attcgagcca aattgctctg tggccgcgcg
     8221 gcggattgtg gcgcggccgc tggtgatttg gcctgcgaat tggcttgaag cggccggggc
     8281 gatgcgccag actcttgatt gccagggccc atggactgtg gacttacgaa ccccgccagg
     8341 gccggaaggc agcaacggca gtctgctccg catttgccgt ggtcaccctg gcacttttga
     8401 gttttgaatg cggagtgcgg agtgcggatt gagaaaccag agcggtctgg gcttcattcc
     8461 gcagtgcgta ctccgaaatc cgcacttaca tgagttatca ggtcatagcg cgcaagtatc
     8521 gcccgcagcg ttttcgggat gtcgtgggac aggaacacgt cacgcagacg ctggcccatg
     8581 ccatcgagca aaagcgcatc gcccacgcct atttgttctg cggcccgcgc gggaccggca
     8641 agaccaccat cgcgcgcatc tttgccaagt gtctcaactg cgaggacggg ccgaaggtcg
     8701 agttcgagga gaaggaccag cgttgccagg agatcgccga gggccgctcg cttgacgtgt
     8761 tcgagattga cggcgccagc aacaacggcg tcgagcaggt ccgcgagttg cgcgagacgt
     8821 gcaagtacgc gccggccagt tcccgcttca agatatacat tattgacgaa gttcacatgc
     8881 tgtccacggg ggccttcaat gctctgctca agaccctcga agagccgccg gcgcatgtga
     8941 agttcatgtt cgccacgacc gacccggaga aggtgctgcc caccatcctg tcgcgctgtc
     9001 agcggttcga cctgcggcgg attccgacgg cgctgattgc ccagcacctc gcgcacatcg
     9061 ccgatctgga gaaagtccag gttgcgccgg ccgcgctcca cgcgattgcg cgcggggctg
     9121 agggcgcgat gcgggacgcg gaatcggcgc tcgaccagtt gattagcttt tgcggcggcc
     9181 ggatcgagga ggcggacgtg ctgtccatgt ttgggctcac ggcgcagggc caactgctgg
     9241 agctcacccg gatggtgctc gccggggaag tcgaaacggc gttgcgcctg ctgaacgatc
     9301 tggccaatca cggcaaggat ttgcggcggt tattatccga cctgctgaat cattttcgca
     9361 acctgctgat cttccaggtc tcgcgcggcg aactggatat gctggaggtg tccgaggccg
     9421 aagcggcgga gctgaaagag caatccccga tggccggcgc ggacgcgctc acgcgcatta
     9481 tggaagtctt cgccgaggcg gagcggcagt tgcgcgacgc cgcctcgaag aagatcctgg
     9541 tcgaggtggc cctgctcaga gcgattgaag cgcgcgccgc ggtgcgcgtg gacacgctgc
     9601 tgcagcagct tcagagtttg cgcggcccgg cggaaacgcg cccggccccg gcgggggctg
     9661 ccccccccag tcatttcgcg ccggagcctt ccgcgtcggc cggttcgccc aagccggcgg
     9721 cgggtggcaa aaggcgcgcg ccggacgcgg ctgcggaggc cgaaccggcc ggaacggcgg
     9781 cggcggctga agaactgccg ggcgtcgctc cggggtctcc aaggcggacc ggtgacctgg
     9841 gcggcctgtg gagcggcttg ctggaggctg taggccgcgc cagccagttc atccgcagct
     9901 acttgctggg ggcgcacccg gttgcgtttg cggagaacgt cctgacgatt ggctacgatc
     9961 ccgagtttgg ggatcacctt ggcctggtgg ataccccccg cacccatgcg ctgctccaga
    10021 ccaagctcgc tgagttgggt catcccaatg tccagatcaa gttcatccag accgaggcgc
    10081 tcgccagccc tgctttgccg ccagacacgg cctcccctcc gccggcggtg agcgccaccg
    10141 cccggcgcac ggcggtcaag cccgccgcgt cggccgcaaa caagccggag gcggtgccgt
    10201 tcagccagga agatttcaag aatgacccgt tgatccagaa ggcgctggag attttcaaag
    10261 ggcggatcgt ggaggttcgt gcctgacggg ccatgatttc ccgcaaccat taaccataga
    10321 gcattacccc aaaaccatgt cgagcatagg caaattgatg aaacaagccg cgcgcatgca
    10381 gcagcagatg gagcaaatcc aggccgccct ggccgcccgc acggttgaag ctaccagcgg
    10441 cggcggcgcg gtaaaggtgg tggcgaagtg cgacggcacg atcgccgcgg tgcggattga
    10501 tccccaggcg ctgaacccgg aagacgcgca ggtgctggag gacatgatcc tgacggcgac
    10561 caaccaggcc ctggcccagg cccgggagat ttccaacacg gaaatgggca aggccaccgc
    10621 cgggttcaat ctgcccgggt tgacttcgta gcggcgggtt ggcgcgcaga attatgcccg
    10681 ctccgcgccc cggatttccc ccgttttccc atggccgccc tgcctgaacc gatcgcgttg
    10741 ctggtggacg cgctgagccg gctccccggc attggcccgc gttcggcgga gcgcattgcc
    10801 ttgcacctgg cgcaggcgga gacggacgcg gtcggaaaac tagccgaggc cattctccag
    10861 gcgcgcgcgc gggtccggtt ttgcgaggtc tgcggcgcgc tcaccgagtt gtcgccctgc
    10921 gcgatttgcg ccgacccgcg ccgggacggt tcgctggttt gcgttgtcga gcggccggtg
    10981 gacattctca gcctggagaa ggcgcgcact ttccgcggac ggtatcacgt gttgggcggg
    11041 cgcatttcgc cgttgagcgg ggttgagccg gaagacctgc gcatcgccga gctggaaacc
    11101 cgcctggtgc acgggcaggt tcaggaaatt atcctggccc tgggaacgga tgtggagggg
    11161 gaggcgacgg gcttttacct ggctcagcga ctcgcccgtc ccgggcttag aatcacgcgc
    11221 gtggcctacg gtctgcctgc gggcacgggg ctggaattcg ccgatgaatt gaccttgagc
    11281 cacgccctgg aggggcgccg ggaaatgccg ggataaaccg cctgccgccg cccggaatga
    11341 acccctgact tttaacatgc ctgctcccgc tgctgttgtt tttgacttgg gcaaggtgct
    11401 ggtggatttc gactaccaca ttgcggcccg gcgtattgcg gcccgcagcc ggatgtccct
    11461 ggttgaaatc gcgcagttta tcaaccagtc ctccctgttc tatcaatatg aatccggcgc
    11521 gttgaccacg cggcagttct tcgaggagat ttgtcaggcc accggcttcc ggggcagcca
    11581 ggctgagttc ggcgcgagtt ttgccgacat ctttgtggaa atcgagccca tgatccgact
    11641 gcaggcggag ctgcggcagc gggggattcc cacttacgtt ttctcgaata ccaatgaatt
    11701 ggccatcgag catatccggc agcggttccc tttctacgcc catttcgacg gctatattct
    11761 atcctacgaa cagggcgcca tgaagcccga tgcgcggatt tacgaagttg tcgagcgcca
    11821 ggcgggccgg cgtggcgccg accttctcta tctggacgac cggcccgaga acatcgctgc
    11881 gggcgcggcg cggggctggc aggtcatttt gcaggaatct ccggagaaaa gccgggcggc
    11941 tgtccggaag ctcggcctgc ttaaccccga ttagcgcccc gccgtcttgc tgatggagcc
    12001 ggcgacggcc agaattccgg tgcgctcgca acgccaggcg atggactgga gcctggtgct
    12061 gatcagccag gggattgagg tcacgattga tcccggcgac gaaggcgctg gctgggggct
    12121 gctggtgcct cggcaggatt gcgcgcgggc gctgcagacg ctgcgccagt accgccgcga
    12181 gaatcgcgcc tggccctggc agcagccggt gtttcgcccg ggcctgctgt ttgattgggg
    12241 cagcctggcg tgggttttgt tgatcggcgc gttttatggg ttgaactccc ggggcgacct
    12301 gcaagccgtc ggcgtgatgg atggcgctgc agtggcgcag gggcagtggt ggcggctctt
    12361 taccgcgatc tggctccatg ccgacttggc gcacctggcg accaatgcgg ccctcgggct
    12421 ggttttgctg ggcctggcta tggggcatta cggaacagga accggcttgc ttgcagcctg
    12481 cctggcgggc gcgggcggca accttttggc gggcttgctt tccttgcaaa cccatcgcag
    12541 cctgggggcc tcggggatgg tcatgggcag cctgggactg ttggcggtgc aatcattttc
    12601 tctttggcgg acgtccaccc gcgccgtgaa gctggtggtc agcggggttt gcggcgggat
    12661 catgctgttc gtgctgctgg cgcttacgcc gggaacggac atcgtggccc acctgggcgg
    12721 cttcctcggc ggactggtgc tgggcgtctt gttgagcctt gctccaaaaa tggctcacaa
    12781 actttatgcg aacctggcct gcggggtggc ctttgccatt ctggtcatcg tcccgtggtg
    12841 gctggcgttg tggggggccg gccgctgacc gcgccgttcc aaggccgttt tatttggttg
    12901 ccaaccccgg cgcagcaact agattccccg tcggtttatg gtcgctcgca gaaaacgtca
    12961 tcacgccgcc cggcgcaagc cgcgctccgc tccgcgttcc gcgcggacga aacggcgcgc
    13021 cccggcggca aagaaacgtg attttcccgg ccggaagccg gcgccgcccg ccaaagatca
    13081 ggcggacgag gagcttctca agattaaaga aacggatctg ctgcctcttg acctggagcc
    13141 ggaagccgat gaccaggttt cagcgcgcga tcgggagcgt tcgagttatg atggcgacac
    13201 ggcgatcaag ctttatctgc gcgaaatcgg ccaggtcaag ctcctgaccc cggaggagga
    13261 aatccagttg gcggcgcgca tcaagcggcg cgacaagaag gcgcgcgagc aaatgatcaa
    13321 ggccaacctg cgcctggtgg tcaagatcgc ccgcgattat gaaggcatcg ggctgccgct
    13381 gctggatttg atcagcgagg gcaatatcgg cctcatgaag gccgtcgaac gctttgaccc
    13441 ggccaagggt ggcaaacttt ccacttatgg ggcgtggtgg atcaagcagt ccatcaagcg
    13501 cgccctggcc aaccaatcca agacgattcg cttgccggtg catttggtgg acaaaatctc
    13561 ccggatgcgc cgcacctccc tgcggttgca ggaagaactg ggccgcgagc cgacggatga
    13621 agagctgggc gaggagttgg gcatcagcgc cgcccgggtt tcccaaatgc gcatggccgc
    13681 cgtgcggccc gcttcactgg atgccccgat cggggatgag gattccaaca acttctccga
    13741 ggtcgtcgag gatgagaatg ccgatacacc ctatcagcag cttgaggata agacggtcac
    13801 ccggatgctg cgggaaatga tcaagaccct cgaccatcgc gaggcgacca tcctgcgcgc
    13861 ccggttcgga ttggacggcg gcccgcagaa aacccttgag gaagtcggcg ccaagttcgg
    13921 cgtgacccgc gagcgcgtgc gccagattca gaatatagcc ctgaagaaac tgcgcaagat
    13981 gattgaaaag atggaagcca tcaaggcctg agccgggtaa aggaatcagg ccccccgtca
    14041 gtccaaaagg aaaaatgagt ctaacgcgtc caacccccgc cgatcttgaa aaggccattc
    14101 gcaatatccc cgacttcccc aagccgggca tccagttcaa ggacattacg ccggtgctcg
    14161 ccgatgcgcg gcttttttcc ggcagcattg acctgttgac ggagcggttc cggcccggcg
    14221 acgtggatgc tgtggtggga attgacgccc gcggcttcat tttcgccgcc gctgccgccg
    14281 tcaagcttca ggccgggttg gtgccggttc gcaagcaggg caagcttccc tatcgcaccc
    14341 atgagcagca atataatctc gaatacggca cggcgaccgt agcggtgcat gtggatgccc
    14401 tcaagcccgg cagccgagtg ctactcattg atgacttgct ggccaccggc ggcacggccg
    14461 ccgccgccgc cgcgctcgtg cgccggttgg aggcccgcat tttggaaatc tccttcctga
    14521 tcgaactcca attcctgggg gggcgccaga aacttcaaga ctacccggtt cgctcgctga
    14581 tcgtgtattg agtaaaggtc tgtttgatgc actggctgca aaccctggac cttgggctat
    14641 tccggtttgt caacctcaag ctggccaatc cgatctttga tctggtgatg ccttttgcca
    14701 gcggcaaccg gttttttgcc ccggtgctgc tgctggcgct ggttctgctg gcctggaaag
    14761 gccggacccg gggcgcgctc tgcgtcctga tgctggtctt gatcgttctg cttggcgatg
    14821 gcttcatctg caaacccctc aaacgaaccc tcctgcgccc gcggccctat gccgccctgg
    14881 cggaagttcg ccgccccacc cgcgagttta gtttttccac tcccgtcgaa actgccgcgc
    14941 tgcggttggg ggaccgagcc agcctgccgt cggcgcacgc cgccaattgg ttcgcgggca
    15001 cgatggtcct cttcatctat tatcggcgca gcgtttggtt catgctgccg gtggcggtgc
    15061 tggtgagctt ttcccggatt tacaacggca gccattatcc cagcgatgtg cttgtggggg
    15121 ccatccttgg ctccgggtat gccgccgccg gcgtttggtc tctgaatgcg ctttggcaaa
    15181 gggcggggcg caagtggttt ccgctttggt ggcgggctat gccatgcctg ctcgagcctg
    15241 aacggcgcgc cagcgatgcc ccagtgccgg attccggcgc gcgctccctg gcagcggcgg
    15301 ccggtgcgcc gcatgccacg gtggaccagc attggctccg gctggggtat ctctggattg
    15361 cgatctccct gctggcgcga tgggcttacc tggccagcgg gattattcaa ctcagcgagg
    15421 acgaggccta ccaatgggtc tggtccaagc atttggcgct ttcctactac agcaagccgc
    15481 cgctgattgc ttacacgcaa ttccttggga cctggctgtg gggcgatacg gcgttcggcg
    15541 tgcggttttt ctccccggtg atcgccgcga tgatcggtgt tatcctcctg cggttcttcg
    15601 cgcgcgaggt caacgcccgc gcgggctttt tcctgttgct catcgtgagc gccacgctgc
    15661 tgttgtcggc gggcgccgtt ctgatgacga ttgatccgct gtccgtattg ttctggacgg
    15721 cggcgatgct ggcgggctgg cgggcggtgc aggaggacgc ctccacccgg ccatggctgt
    15781 gggtcggcct gtggatgggc ctgggattct tgagcaagta cacggaattg tttcagtggc
    15841 tctgctgggc agtctttttc gctctctggc ctgccgcgcg ccggcatttg cgccgcccgg
    15901 ggccctggct ggccctgctc atcaacctgc tatgcgcgct gccggtgctg atttggaacc
    15961 accagcatga atggatcacc gtccgccacc tggccgaatg cgcgggcgcc gatcgtccgt
    16021 ggcaccccac cctccggtac ctgggcgagt ttctcggcgc cgaactgggc ctgctgaacc
    16081 cggttttctt tggcgccacg gtttgggcgg cgatcgcttt ctggcgccgg ggccgccatg
    16141 atccgcggct ggtctttttc ttcagcatgg gcgcgccgct gtttgcggcg tacctgctgc
    16201 attcgtttcg ctcgcgcgtg ctgccgaact ggatcgcgcc cgccgtcctg cctttattct
    16261 gctttatggt catttactgg gacacccagt ggcggctggg ccgggcccgg atcaaatcct
    16321 ggctggccgt cgggctgggg ctgggatttg ccgccgtgct tctgggacac aacaccgacc
    16381 tggtcaaagt attaaccggc caacggttac ccgtaagcct ggattcgctg cgccgggtgc
    16441 gggaatggga ccaagtggcc cgcgttgtgg gagccgcgcg gcaggaattg cttgccgagg
    16501 gcaagccggt gttcatcatc ggcaaccatt atggcatggt ggggcagctt tccttctacc
    16561 tgcctgaagc ccgggccgcc gtgacgaaag aacccttggt cttcttccgc gccagtccaa
    16621 tacccaaaaa ccagttctac ttctggcccg gttacgagaa tcgccagggc cagaatgcca
    16681 ttttcgtgcg cgagttgaag cgggaccagg aaaacacccc gcccgcgccg ccggatttgc
    16741 agcgggcgtt cgagtcggtg acgcccctgg gcgtgcgcaa cgtgctttat gggggccggg
    16801 tggcgcgccg cctccaactc ttcgcctgcc gggatctgcg gtgaccggtt gggcaacggc
    16861 aagctccatt cctcgcgggg agcgtaatct ggccgcctcg tgcatccggc ccaaagcgcc
    16921 gagccgggcg ggcgccatcc gctaaaaaaa cccggctccc gccgccgccc ttcatttcgt
    16981 cccccgcagc aaaatcgcgg gattggggtg gatagcttgt caaagacgcg gggcgtgagt
    17041 aatgttttgc catgttaacc ttgcggcccg tgttcgcagc attcctggcg gctggcctgt
    17101 cctccgcgat ggcggacacc atccagttga aggagaatgc ggccatcacc ggccggatcc
    17161 ttgcggagaa gccggatcaa atcgctgtgg atgtcggcta taccgtgctg gtaatccccc
    17221 gcaaccaggt ggccaaggtt ctccgcagcg atgccccggc gtccgcggcc aagccccggc
    17281 gcggggccag gggtggggag gggagcggca gcgtggagac tgacgaagcc cggccgggtt
    17341 tctattccgc ggccggcaag cccgccgcca tccgcaccgt gctggaatta gcgacccaac
    17401 tcggcggagc ggtcgtgcaa atccgcacgc ccggcgggct cggttccggt ttcttcatca
    17461 acgaggacgg tttcctgatc accaacttcc acgtgatcga gggcgagagc cagatttccg
    17521 tcgaggtcta tcaccagcaa ggcgggcaac tcgaccggca aacttacaag caggtgcgga
    17581 tcattgcgct caacaagttc gctgacttgg ccttgctgaa agtggaggat aaggatgcgc
    17641 cccggtttcg cttcgtcccg ctgggcaatt ccgatgcgtt gtcggtgggt gagcgtgtat
    17701 ttgccatcgg cagcccgctg ggactggagc gcaccgttac ggaaggcatc ctgagcacca
    17761 agacccgcga ggtgggcggg ttgctgtatt tgcagacgac ggcgcagatt aaccccggca
    17821 acagcggcgg ccccttgttc aacctgggcg gcgaagtcat cggcgtcatt aatatgaaag
    17881 tcactgccgg ggaggggctc ggctttgcca tcccggtgga gtcgctcaag cttttcctgg
    17941 atcaccggga tgcgtttgcc tattccaatg acaatcccag caacccctac cgttatctcg
    18001 aaccgcccgg ccgcctgcgg caaaatccgg tggaagagta agctcggagc ttttgggcgc
    18061 gagtttgggg ggcgcgcagg ccggttgcga atcggcgccg ggttcgtggg actgatcctg
    18121 gcagccgcca ccctcggagc ggcgattccc ggcccggaac gattgctgcc ggaggatacc
    18181 ctcctcgtgc tgacggctcc tgatttcccc aagcttcttc aaatttgccg gcaatcaccg
    18241 aagggccggc tttggaatga cccggttatg aagccgctgc gggacaagtt caccgcccgc
    18301 tggcaggagg aagtcatcaa gccgttggaa cgggagctca acgtgagcct ggacacttac
    18361 cccggcctgc tgcaaggcca gttgacgttg gccgtggtcc gaaacactcc gccagacgaa
    18421 gatcattccc cgtttggtta tctgctcttg cttgataccc gggacaaagc cggggtgctc
    18481 cgaaccaacc tcgcccactt gcgcaaacaa tgggccgccg ccggcaaaac cctcaagacc
    18541 gagcccattc gtggcctgga gttctccgtc ctttccgtct ccaccaacga aatgccgccg
    18601 acgctgtcga aatggctttg gcacgcgccg gtatttcccc cgctgatcac ctatcccgag
    18661 ctcaagcagg ggcccgtcag cccgtcccgc aaggatgatt tcgtgctcga cctgctggtt
    18721 gaactgctga ccgccaatcc ggatttggtc ctgggccagg tggattcctt gcttgtcgcg
    18781 ggcaactcgg tccggaacgt ggagcgcgtc gttgcccgcc tcacgggcgg cacggccccg
    18841 gtcctgggcg acgcgatcgc ttatcaaacc agccagcaag cccatttccg cgatgctcct
    18901 tgctatggct gggtcaatgt caaagccctc gcggatagcc tgagccgagc cgctgccgaa
    18961 tccgccgccg ccgaaccgcc tgatcccctc gagccgctca agccggacca ggtcatcagc
    19021 gccaccggcc tggccaactg ccaaaccctc gcgttcagtt tccgggattc caacgccggc
    19081 agcctgatcc actgtttcct caccgtgccg gacagcgccc gccgcgggct ttttccggcc
    19141 ctggccggcc ctccaaagga tgccagtccg ccgcccttcg ttccgtccga tgtcgcgctg
    19201 ttctttcgct ggcgattaag ccttcccgga gcctggacgt ctttcgagca gatgctcaag
    19261 gacctttcgc cgccggcgtt cagcgcgatc caattgatcc tcgatactgc caacgcccgc
    19321 gcccaacagg atgaaccggg ctttaacctt aagcaggcgc tgctggtcaa cctgggtgac
    19381 gaccttatca gctacgagcg ggcgccccgc agcggttccc cggcgccgca aaccacccct
    19441 tcgctttggc tgctcggttc gcccaatccc gaactcctgg cggttgccct caagcgcctc
    19501 tttgtgatct ttccccaggg cgacgcgttc gccgagcgtg aattcctggg ccggaagata
    19561 tactccgtcc ccgcgccgcc catgccgttc gctacggcgg cggtttcccg tccggcgccg
    19621 ccgcgcacgt tgaattgggg cgccagcgcg ggctacgtcg ccttgtctac cgacatcgct
    19681 ctgctggaag aatacctgcg cgccgaccaa aaccaaatca aacccctccg ggacaagccc
    19741 gggctattgg aggcggcgca gaatgtcggg ggcatgggca ccggcctttt ccggtacgaa
    19801 aatcaggccg tcacaatgcg cgcggttttc gaggccgcga aaaacgacct gcaggccgca
    19861 accaatggct ttggtcccag cctgtttccc gcgctgccgg gcatcaccgg gccggaaagc
    19921 aatctcagct tctggatgga cttctcgctc cttccgccct ttgaccgcat cgcgcagtac
    19981 ttccctttca gcctctacac tttcggcgcg aatgtggagg gctttacctt gaagctattc
    20041 atcccggcgt ccccttccgc cggcgatagc gccccggccc aaccggcaaa ctaatacgct
    20101 acggctttgc gtaattgcgt ccgccggtcg gcaactcggt cgagcccgag ggcggaacat
    20161 tggtcggcgt cgtaatcagc ggaaagtaag gcggccctac cgccgggcct ctggccggcg
    20221 gaaccggcaa ttccgggagc ggggttggcg tcgcgggaac ctcgggcgtc accacgggcg
    20281 tcacactggc aatcggggtc gagggagccg atggcgcaac cggggaaggg gcggacggag
    20341 gtgtcacggc agctgggccg gtctgacccg ggttttgcgc cgccattccc gcttcggcca
    20401 gcaccccgcc caccgacgca atcttgccct gatagccggc cagaaccttc tccagggcag
    20461 gctgcagggt tggttgggcc gcgcccaccg cttgcacaat ctcccggttc gcttccggcg
    20521 caacttgcga aaccgccact gccacgttgg ggtaatacgc cggcgccacc cggcataccg
    20581 ccgccactat cgcgctggcc tgcgccggcg ctgccgccgc ggcggctttg gcgatcgcgc
    20641 tggcctgcct gggctgttcc acggcggctg ctgccgcggc gacagcagcc atatccggca
    20701 ccgcgtgcgc gatggccccc acaatggagg tggcggctgt gggattaatc cccaacgcgc
    20761 tcttgaccac atcaagggtt gtcgccttgc ggtccgccgg tttggctcgc aggaccaggt
    20821 ccgccgcttt agccgggagt tccgccatgg gaacggcggc cagtgccatt cggctggagc
    20881 tggctgtggc tgcatccttc ccatacgagg cgcaagccgt taataacgct gcgcaaaacg
    20941 caatgctggt aatccgtttc ataatattat cccgacggcc ctcggcgggc cgaacgtgac
    21001 tctgtaccct tatttctaac caattgcgcc gcccgcgtca accttttaag ttaggttttt
    21061 cgcggccggg tttccggcga tccgccggag ctggccccgg cctcagcgtc cggttttggc
    21121 ctttgccttg ccccgggaag gctgcaggtt gaggctgtct ttgatgcgtt tgatatgtcc
    21181 gcggcccatg cccttgacct cgcaccccag ttccaggtag cgccgaatct tctcgtcatc
    21241 gagcacgcag aagcaatcca caacggcaaa aggctggccg gcgcttttca ccacccggtc
    21301 cgggtcaagc ttcaagtatt gatcgtgccg caccgccagc acgagggcgt caatgccttt
    21361 caaggccccc caaagatcct tttccaggcg caggttcttc agcggcgcct gccgatggaa
    21421 gaaccgggcc cagctataat tggggcgcgg ataggtgtcc tgcgcctcga actcccacca
    21481 atggtccagg taagggtcat ggactttcac ctcggccccc atttcggaga gtttgcgcac
    21541 gatgatttcc gagccgctgt agcgggtatc ccccacatcc tcccggtagg cggcgcccag
    21601 cagcagcact tccgccgccg cgatcggttt gcccatgttg cggagggcat cccgcaccag
    21661 ttgcggcgcg tgcagcgacc gggtgtcgtt aatatcaatg gccagcgggg taatcttgaa
    21721 aatgtcatcc tcgaagccga aaatatggcg gtaggcccag acccccagcc cgccgtcttt
    21781 gggcaggcaa tagccgccaa tgcccggccc cgggaagatg atgttggagt gggtgggccg
    21841 aaccttgatg gcctcgatca ccttcttcaa atccacgccg ttgcgctcgg caaagaggga
    21901 ccattcatcc aggaacgcga ggatggtggc gcggtagctg ttctccacga tcttggccgt
    21961 ttccgattcg atgggccggt ccagcaccgt cagcggatac cggtcggtgt gcagcacctc
    22021 gttgagaaac cgggtgacgc gcttgcgcgc ggcggggttg acgccgctgc acacgcgcca
    22081 gaaatcacgg atggaggcca cgtagttccg gcccggcata acccgctcgt agctgtgcgc
    22141 cagcagcggc tccgaacgaa tcccccgctt cgtgaaaatc tttttcatga tcgggtaggc
    22201 gatctgctcg gtggttcccg gggcgacggt ggtttctatc aggaccagcg cggcgggggg
    22261 aatgttttcc gctatggtcg ccattgacgc ttccagcgcc gccatctccg ccgaacctgt
    22321 gcggacattg cccagggatt ccttgaggta atcgcactgg acgtccacca ccaccacatc
    22381 ggccagcttc aacgcctcct cggtataggt ggccaccagc gtctgcttct cattaacgca
    22441 gcgggcgatg atttgctcca cctccgggtc ttccgcttta accggggaaa caccccggtt
    22501 gagcaggcgg attttccaaa aactgcgcgt gctggggcgc tgcatgccga tgacgaactt
    22561 cgtcgggcgg cccttcttgt cggtggcgtc ggccaccacc gcggccatga ccgcgcctac
    22621 aaatcccagg cccaccacca ccacgatctc ccggccggcc tggcgatgcc gggcggccag
    22681 ctttttcaaa cgggcgaact cggccgggta ttcctccggc aatggcagcg ggaactcctc
    22741 gccggcgggg ctgattgaca cgggtttctt ttccatttgc gacggtttca tatctcgttt
    22801 ggtttggccg tatctctgtc agaaatcccg gcgcctggcc agcccggaat cagccggggc
    22861 taatcgccaa ggctgatgcc cagggcgcgc gccgtttgca ccaggtcccc ctccagcgga
    22921 accaggcgga gccgcccgat cgcttcggtg atggggacgg caatagtgtc gggcggccgc
    22981 agggcgacca tgtggccgta tttctcctcg gcgatcaact gcaccgcccg cgtgccaaaa
    23041 cgcgtgcaaa gttgccggtc gaacgaagtc ggcgcgccgc cgcgctgcaa atgccccagg
    23101 acgcacacgc gcgtttcttt gcccgtgcgc ttttgaatcc cggcggccac gacagcgcca
    23161 atgccgccca gccgggcttc gcggctcgcc tccgcccctc ccgcggtcac aaagtccgcc
    23221 ccctgctcgc gcgccccctc ggccaccacc accagggtga aacgcttgcc ctcgcgctcg
    23281 cgctcgatga tcttcgcgca cacgcgctcc aggtggaagg gaatctccgg aatcaggatc
    23341 acgtcgccgc cgcccgaaat gccggcggtg atcgcgatcc agccggcgta gcggcccatg
    23401 acttccagca ccattacgcg gtcgtggctc tgggcggtcg tgcgcagccg gtccagcgcg
    23461 tccgtggcgc aggccaccgc cgagtcaaag ccgaaggtca gcgccgtggc ggccaggtca
    23521 ttgtcaatag tcttgggtac gccgataatc ggcaccccct gctcgaagag ctgctgcgcg
    23581 gtcgtcagcg agccatcgcc gcccaccacc accagcgcgc gcaagccaag ctgctgaatg
    23641 ttcgctttgg cccgccgcag aatgtcgcgc gggatgcggt gggtttcgcc gtggccggtc
    23701 ttggccgcga accgcccctt gttggacgtg cccaaaatcg tcccgcccac aaaaagcagc
    23761 gcgtccattt ccttgtagtc aagccgccgg tagcgcaccg gctccagcaa accctcgaag
    23821 ccttcgagga atccgatcgt ctcccaaccg tgcttggtcg cacacttgac caccgcccgg
    23881 atgaccgtgt tcagtcccgg gcaatcgccg ccgcctgtca aaatgcctat ccgttcccgc
    23941 atggccggaa cgtagcaacc ggcggcgcgg aaccaaagcc caaagtgcgg ggttttttac
    24001 ccgttccggg ggcgccggac accgtggcac ggcccgaaaa agacgggctc acgtccgccg
    24061 ccggtcctcc ccggcttccg cgcgggcgcg gttcgccggg gcaggcgtcc ggctaatcct
    24121 gaacctcgat ccggtagaac cgctgcggaa tcggatcttc gtaggcaatc acgctatccg
    24181 tggccaccgt ggtccagtcg gctgcggtga cgtccatcag gtcggtccac tccgcatcgg
    24241 ccaggttcag tttgtattgc acgcggtagg tcagccccgg gatcgaactc caggtcaggt
    24301 tgatgaaatc gccggccaga accgcgctca ggattgcagg ccgggccatt acggtgatgc
    24361 tgaaactccg ggcatcgccc aggctgggcg tgccatcatc gctgacccgg atcgtgaccg
    24421 ggttggtggt gttgaggctg gtctccggcg cggtccagcg gaacaccccg gtcgccgtgt
    24481 ccagcgtggc ctccgccggc gcgcccggct ccaggctgaa tgtaagcgtc tggtccagat
    24541 ccgggtgggt gacggagctg ctaatcacca ccgtgctgcc cacgtggatc gtgcgatcgg
    24601 gaatcgccgc caggatcggc gggttattca ccgtcagcac cgccggatcg ctggtgacgc
    24661 tgccgaccgc gttcgcgacg gtgacggtat aggcgccggc atccgccgtt tgcacgttgt
    24721 tgcgggtgca ggcagaagcc gttgcgcccg cgatcgggtc gccgttcaac ttccattggt
    24781 aactcagcgc ggtgcccgtc gcttccacgc tgaacgtcac cgtcgccccg gccgccaccg
    24841 cctggcccgc cgggttcagg gtaatgaccg ggggattttg caccaccagc accgccgctt
    24901 cgctccgcac gccgcccgcc gcgttggtca cgccgaccgc gtagctgcct tcttcggccg
    24961 ccgttacccc ggccagcgtt agcgtgtccg tctgcgcgcc ggaaataatt ccgtcgtcgc
    25021 tcaaatccac gccgtccttc tgccagcggt aagcgagcgg cgcctggccg gtggcggcca
    25081 ccgtgaagct gaccgtgctg cctgcctggt tggtttggct ggccggttgc agggtgattt
    25141 ccgggggcat ccccagcacc gtcaacaccg ccgcggcgct caccaccggc cccactccgt
    25201 tgctcacctg gaccgtatag gccccggcgt ccgcttcctg cgcgttgttc accgtcagcg
    25261 tcgcttccgt cgcccccgcg atgttctccc cgttgcgccg ccactggtag ctctgcggat
    25321 tgttcgtcgc cacca
//
LOCUS       DAOPQG010000050        24835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2539_length_24835_cov_15.014205, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000050 DAOPQG010000000
VERSION     DAOPQG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 24835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24835
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2539_length_24835_cov_15.014205"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..1289
                     /locus_tag="PKI07_07440"
     CDS             471..1289
                     /locus_tag="PKI07_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOC50924.1"
                     /translation="MAVFYDTHAHLGRREFAEDLPQVIARAEAAGIGRIITIGTDQAS
                     SERAVELAERYPQVYAAVGWHPSQAAEAPEDFRPALRELAGHRKVVALGETGLDYYRL
                     PRRPEGAPAGAEARDKARQADLFRQHLEVAAETGLGCIIHQRSSLEDTLALVRPFAGR
                     VRGVFHCFAEDAAAGRRIADLGCLVSFTGIVTYKNAPLVRETLAATPLGQFMLETDCP
                     FLAPMPYRGKRCEPAYVREIAAAVAQVKGCSLDELSAATCAAAEKFFARPAAGG"
     gene            1497..2510
                     /locus_tag="PKI07_07445"
     CDS             1497..2510
                     /locus_tag="PKI07_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC50925.1"
                     /translation="MKHDEFAFFNQQLAAMLREDIPLEGALRQLCAGMRKGPLRDELQ
                     RLEADLAGGTPLPDALSRRALPELYRRMVTIGVRGNDLPGVLLLLADHYHRHNTLWAR
                     LKGLLLYPLIVLVVALGLTLVLTLILKWFLAEIRGAPELLEWGGRWRLFGQWPVEILI
                     WFPPIALALGALAGVVAATLPACRARLRWRLPAFREASLAQLASAIALMLKNGATLPE
                     ALALAEALESHTPAAAPLARWRRRLEAGQGKPADWDAENRPFPPLFLWLVQRSGEDVA
                     AGFQQAADLYRARASYRIEMMLYGALPVAIVLLGLMVLWQAAPGVIWLTRLGDFLYLV
                     GSF"
     gene            2522..3718
                     /locus_tag="PKI07_07450"
     CDS             2522..3718
                     /locus_tag="PKI07_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC50926.1"
                     /translation="MSVNDVPLLLAALLPDLGAGIMTLLAYLVIGLAPAVGIAYAIYV
                     LLTLPLRRNERARMFLNLLELGIKEGQAPEAVIASASASRDRSLGVRFHWLAAHIEQG
                     LRLSEALERVPRLLPPQVCAMLRIGERIGEVQKVFPACRWLLRDSVSQVRGALNYLLL
                     AVFAITPGLFIILFWLKKALLPQFMAIYASLEEGMSLPAFTRLIFNDSPALLAIQLGV
                     LAILWGVTLAYLGGPRLHGWIHRLAPGLQDGFLARLPWRNHRLRRDFSAMLALLLDAG
                     APEAEAVRLAAESTANQTFIRRGQRVCARLQQGVSLPEALRAMDNTPELHWRLAHALR
                     RGAGFVRALGGWHEALDARAFQWEQAAAQVLTTGLVLVSGLVVGGFAIGIFLVIVQII
                     NTGCLW"
     gene            3712..4113
                     /locus_tag="PKI07_07455"
     CDS             3712..4113
                     /locus_tag="PKI07_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50927.1"
                     /translation="MVITASLVHGFRPPRRGRQRGALMTELLVALAILMGTLLPLAYA
                     FAAEHRLARACYQRAVALEIVDGEMEVLLAGGGRAFGPGTYDYPVHARAATNLPAGRF
                     ILTVQPGKLRLQWRPEPKKHGGSVVREAVFP"
     gene            4110..4640
                     /locus_tag="PKI07_07460"
     CDS             4110..4640
                     /locus_tag="PKI07_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50928.1"
                     /translation="MKTGCACRSGARRAGRAAYLLIEALVYIAVLGGVLGAGYLALYR
                     GLDRSIALRRNADEITSALHAGERWRADVRSATAPPRLGTTEDGQFLYLERPARSVAY
                     RFATNAVFRQSGGGGWVCLLRHVESSVMAADPRERVVAWRWELELRPRPSGSVKPGRI
                     RPLFTFLAVPEKPAAP"
     gene            4637..5056
                     /locus_tag="PKI07_07465"
     CDS             4637..5056
                     /locus_tag="PKI07_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50929.1"
                     /translation="MKLPSVHRIRSSRRESGVAVIVILALLAILMIYVAGNLRTLDHL
                     EREVRLLERKQILRLQATARPASSAVEAPGESRGLRPPSPVSATAEGAPPAGEGRVRE
                     DHERTGSENSLPAAPPAANDPPQVRGTADPSTPESPP"
     gene            5053..5451
                     /locus_tag="PKI07_07470"
     CDS             5053..5451
                     /locus_tag="PKI07_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50930.1"
                     /translation="MNALSHDNRDGKASGRRGWLFRRLVNPARPKARQPLTGPALRIW
                     LMRRSLATFVWGIFSLLPVIGFSLGLGVLAYWVCMRPYRTREWNPAVVYLRWGIGLAL
                     LGVTGSALILYYWARLVFLSGQYSIYNNLY"
     gene            complement(5520..8084)
                     /gene="hrpB"
                     /locus_tag="PKI07_07475"
     CDS             complement(5520..8084)
                     /gene="hrpB"
                     /locus_tag="PKI07_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOC50931.1"
                     /translation="MAERLPIYELEQPLLTGLQAAGRLIVSAPTGSGKSTQVPQMLLE
                     RGVLGEGQVVILQPRRLAARLLAARVAGERRTRLGGEVGYQIRFENVTSARTRIRFVT
                     EGLLLRQMIRDPQLRGVAALIFDEFHERHLYGDITLARALALQETGRPDLKILVMSAT
                     LETAPLERYLKPCRVIESQGRAYPVDIEFAAAPAYLDLRPVWEQAAEAFAGFVRGGGE
                     GDVLIFMPGGFEISQTLEALRRRAEARGFRLLPLHGELPPREQDAAVARYDQRKVVVA
                     TNVAETSLTIDGIRCVIDSGLARLPRYDPYRGLNTLLIEKISHAAADQRAGRAGRTAP
                     GLCLRLWSRAEHAHRPARELPEVKRLDLAEVVLTLKAAGVEDLRAFRWFEPPEEQALA
                     HAEELLTDLGALAAAPGPHRSGAPARLTPLGRKMLAFPLHPRYARMLLAAQQYGCVHQ
                     ACLLAALTQGRDLLLRHADAEIARRREELPGVSAGSDFWLLRSVWQHVSARDFHPETC
                     RRLGVHAAAARQIGPLFEQFLDLARREGLDAAPRPVADAALQKCLLLGFSDRVARRLD
                     GGTLRCELVHGRRGQLARDSVVRQSPLLVAAEVREVEGPDQSVQTRLSLATAIEEDWL
                     RELFPDALAPALRVFYDPTAKRVYAEEQLHFRGLSIGARRVEPPPAEAAARLLADEVL
                     AGRLELHDWDEAVEQWVLRLNRLAQWCPELALPPLGEAERRHLVEQICHGSFSGRELK
                     NKPVRKLVQGWLSAAQQALLSKHAPERLTLANGRAPKVIYDAANPPHIALRIQQLYGV
                     NATPRIAMGRVPVLVHILAPSQRPVQITQDLAGFWREHYPRIKQELQRKYPRHEWR"
     gene            8185..8661
                     /gene="ispF"
                     /locus_tag="PKI07_07480"
     CDS             8185..8661
                     /gene="ispF"
                     /locus_tag="PKI07_07480"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOC50932.1"
                     /translation="MVHVGIGYDVHALVEGRKLILGGVEIPHPKGLEGHSDADALMHA
                     ICDAVLGALGEADIGHLFPNTDPRWKGAPSRLFLNEAVRLVGFRNGKIVNVDATVIAQ
                     APRLLPHIREMKANIAAALELNIQQVGVKATTNEHLGFLGREEGIAAMAVASVDLP"
     gene            8718..8990
                     /locus_tag="PKI07_07485"
     CDS             8718..8990
                     /locus_tag="PKI07_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50933.1"
                     /translation="MAKAEISWKRTTAEGIRLQVYARHVGREWRFFARERRYELWQAL
                     PEPPLEDWLALLDSVQRRIQRRLLRPEEEARVKQSIRERFPEAALE"
     gene            9063..9839
                     /locus_tag="PKI07_07490"
     CDS             9063..9839
                     /locus_tag="PKI07_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOC50934.1"
                     /translation="MTRPPLIFISPSVEKKGDEFGDLSVSLSETYQQALTDAGGLPLA
                     LPALPSREWIAASVRCCDGVLLTGGDDVDPRIYGRKLPPGLQRTVTLTPDGGARDWRE
                     LVLIDEVFRQRKPLLAICRGLQILNVALGGTLLADIASQRPGAINHRRLDQRSEIVHE
                     VLLTPESLLSKITGGHSLGVNSTHHQAVARVAGPLRVDATSRDGIIEGLELKPGAARM
                     LPFLLGVQFHPERLVKRHREHRAIFAAFVQACARNGKFDL"
     gene            9836..11200
                     /locus_tag="PKI07_07495"
     CDS             9836..11200
                     /locus_tag="PKI07_07495"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC50935.1"
                     /translation="MKGKILIVDDDAGYRELLKGFLEGDYELSEAESGATLKKAMEQG
                     QPDIVLLDVELKDANGLEMLPSLKKRWPETEVIVLTGMPRSDTEAVAWAVAATKRGAF
                     NFLRKTEQFDGGKLLTDVSNALEARRQTEETSMLRRALETMSGSASPIFHSAAMREVV
                     RTVERIAPSDVAVLITGESGSGKEVIADLIHTFSPRSKGRIIKINCAALPRELIESEL
                     FGSVKGAYTGAHTDREGLFRLAEGGTLLLDEISEMPIDTQSKLLRVLQDQEVRPVGGK
                     TAYKTNCRLVAATNRDPREAINDGKLREDLFYRISAISVHLPPLRERREDIMPLANAF
                     LKRFAAQANRVIKGFTASAIERLTAFDWPGNVRQLQNEVQRAVLLSEGNEVDAADLSI
                     STARPGAGDSSDTNFTLLEGVERNAIIQMLKETNGNKLETAKRLGIGRQTLYNKIKAY
                     GIDV"
     gene            11200..11655
                     /locus_tag="PKI07_07500"
     CDS             11200..11655
                     /locus_tag="PKI07_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC50936.1"
                     /translation="MASSAAASSGQAAKGRILIVDDEEGIRELLKALLLDRYLVSEAD
                     SGATLRQAFACDRPDVVLLDLKLPDANGLALLPVIKQQWPATEVIILTGQPDEHGVPS
                     WATEAARGGAFSLVSKSAEFDCQKLLDGISLAMLRSLQARGEHAPSPGA"
     gene            11680..12471
                     /locus_tag="PKI07_07505"
     CDS             11680..12471
                     /locus_tag="PKI07_07505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOC50937.1"
                     /translation="MNLNQARITATRAALAAGDLLRRELHAPKRAQSVLQHDIKLELD
                     VRCQKQIARALKRAFPEVALLGEEGVAGRSDAARRWVVDPIDGTVNFTYGIPHACVSI
                     ALQQRAAGRADYRDGYDTLVGVVYDPFCRELWTAIRGQPARLNGKIIQASRRRQLREA
                     IVSIGFAKSRSSLVATLPYFVKLAHRVRKIRMMGSAALGLTYVACGRFDAYIEREIQT
                     WDIAAGGLVLQCAGGEFWHEALSDPHQHRVIASNGLLRHSLRVPR"
     gene            complement(12547..13923)
                     /locus_tag="PKI07_07510"
     CDS             complement(12547..13923)
                     /locus_tag="PKI07_07510"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HOC50938.1"
                     /translation="MKTLTLAEALAAASETRHLEIGVGVRSAAPRVFQQQFGRQTAVI
                     VADENTFRAAGQAVAEAFGATGHPQLEPFIFRAPDLYAEHRFVAELEASLRGHAAVPV
                     AVGSGTINDLTKLASHRTGRSYMSVATAASMDGYTAFGASITYEGAKQTFQCPAPAAV
                     VADVEVICAAPDQMNAWGYADLVAKVPAGADWILADFVGSEPIHPSAWAMVQGRLREM
                     VSDPAGVRSRNPTAVGKLVEGLLLSGFAMQAAKSSRPASGAEHQFSHLWDMQHHTHNG
                     SAPSHGFKVGIGTLATTALHECLLAQPLEKMDVAQCCAAWRDEAAWVAYAREQLGYNE
                     LGDVAERELRAKHSRPEQLREQLEALKAGWPKLRERLRAQLLPVATLRQMLHDAGAPT
                     EPEQIGITRARLRQSYWQAFCLRRRFTVLDLAVRTGRLAGCLEEIFGPNGLWPISAAG
                     SAPAREQN"
     gene            complement(14162..15352)
                     /locus_tag="PKI07_07515"
     CDS             complement(14162..15352)
                     /locus_tag="PKI07_07515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC50939.1"
                     /translation="MSPHRIVARLALPVILGLSPGIAMGQGNGIFADFVTSKGNFTCQ
                     LDYTNAPRMTANFIGLATGQRAWLDLTTGRARTNAFYNGLTFHRIIAGFMNQGGSPNG
                     MGTDGPGYALKNEFSPKLVFDRFGVLAMANSGPNSDGAQFFITVAPFPAGNNTYSIFG
                     RVVSGSNVVYAINQVPTGANDAPLTPVIIQQVSIRRVGTAAQAFNIHAQGLPIVTNLP
                     LGIAALPGQVSLTFSNRQYADNQLFGTANLAGGWTANALGIETAAPVTNRVLQANDAP
                     RKFFRLAQVQYPSSTFAPKTMYGRTLTLTFTSALTNTLTIAFDSAGGGEYTIPPFDPG
                     TVTEYSWTQEPYRGFLWPIYFSGLVPLTANLAFTSNTNGTFKGTFYSDPPFNVSGNFH
                     LTGP"
     gene            complement(15484..16176)
                     /locus_tag="PKI07_07520"
     CDS             complement(15484..16176)
                     /locus_tag="PKI07_07520"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOC50940.1"
                     /translation="MVSLLIATRNAHKVGEIRAILGEGVRCLTLDDFPGAPEVVEDAE
                     TFAGNAARKAEALARWLAERPNVKPGTETGEWYVLADDSGLEVDALQGAPGVHSARFA
                     ALDTGHPGNSSAADNNAKLLRLLAEVPPVERTARFRCVLALTPVPRFPAGAAAAPGRA
                     GEMMLRTELFEGVCEGRIGLAPRGQGGFGYDPLFIPAGQEATFAELGEAIKNRMSHRS
                     RALALLGAKLRD"
     gene            16470..17999
                     /locus_tag="PKI07_07525"
     CDS             16470..17999
                     /locus_tag="PKI07_07525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOC50941.1"
                     /translation="MPKPITVYLPYSGQEHTRRTLDQLQQSPVIERICLLTAGSGGKL
                     PAGCDRLAVGALHASRTMRMIAQNTRTPYTLLLIHDTTIEFWQYALERMLTVAGLTGA
                     GLVYSDYHDIQGGQRVSHPVIDYQAGSIRDDFNFGSVLLFDSKAFKAAARQRQNFQHA
                     GLYSLWLGVSRLRPLTRVAEHLYGKVELDVRKSDQKQFDYVDPKNRAVQIEMEQAATE
                     HLKRVGAWLKPRFKTPNLDRVAFVHEASVIIPVKNRVKTVGDAVRSVLMQQAPFSFNL
                     IVVDNHSTDGTTDLLRGIAQKDPRMVHHIPERQDLLIGGCWNEAIMHPACGRFAVQLD
                     SDDIYKDETTLRQIVEAFHREKCAAVIGSYRMTNFNLEEIPPGVIDHREWTPDNGRNN
                     ALRIHGLGAPRAYYTPIIREIRFPNVSYGEDYAAVLAVSREYQIARIYEPIYLCRRWE
                     GNSDAGIDVARQNAFNFYKDKLRTFELIARQRLNQAAQPPVRPARKAPARKRKTGKAR
                     R"
     gene            18015..19055
                     /locus_tag="PKI07_07530"
     CDS             18015..19055
                     /locus_tag="PKI07_07530"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOC50942.1"
                     /translation="MTRSLEQIVISPAELARFGTGTTLADQAHALLAQQKGVWEMLRN
                     GYDTLRTVQTRVFDFDRFHIKVQFNPGRMTSTVAKVDATSIKERKCFLCLENLPPAQR
                     GLACEADYLVLCNPFPIFPEHFTISCLRHTPQLIRHSLAAFLKITRDLGARYLVLYNG
                     PRCGASAPDHLHFQAGNRSSLPLDAEFEGMKAACATRLFESAAVRVWSVENCLRHFFT
                     FESANAGALEQSFAALYAVLQEGGPADEEPMLNILGFHTRDEWRIHVFPRAKHRPSFY
                     FLEGEEKLLISPAAVELGGICTTPREADFARVTREHIVELYREVGVAPERFADLKTRL
                     AARLAAAPPIGS"
     gene            complement(19072..20082)
                     /locus_tag="PKI07_07535"
     CDS             complement(19072..20082)
                     /locus_tag="PKI07_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOC50943.1"
                     /translation="MKLIRKRAYSRAGLVGNPSDGYHGKTIAMIVRNFCAEVVLYEWD
                     TLDIVLAENDRARFASIQDLARDVKLHGYYGGIRLIKATIKRFVDYCQARNIQLHDQN
                     FSVRYQTNVPRQVGMAGSSGIVIAMLRCLMEFYHVEIPREVQPSFALPIEREELGITA
                     GLQDRVAQVYEGVVYMDFNPARERVVQGFPSYYYEPLDPALLPPLYLAYHTDFSEPTE
                     VFHNDIRGRFNRGEARVVQAMQRCADLAARGREALLRRDYDELGRLMNENFDTRRSIY
                     NLPPWQVEMVETARRCGASAQFSGSGGAIVGIYQDAAMFKRLSERLGALQCRTLKPQV
                     LP"
     gene            complement(20066..20959)
                     /locus_tag="PKI07_07540"
     CDS             complement(20066..20959)
                     /locus_tag="PKI07_07540"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOC50944.1"
                     /translation="MNEVNIAVVPVAGLGTRLLPATKSQPKEMLPVGRKPGVQYVVEE
                     LTRAGMQRLLFVTGPGKSSIENHFDLNEEMIQNLRETGKEELLEELEFERAPVQYFYT
                     RQRQLLGLGHAVLCAQPFVGDLPFVVALGDSIIGLQAQSDIVRRMTRCFMEQKAEAVV
                     AFEEVPAREIHNYGVAQPRGGGEVFELADVIEKPSAKEAPSNLAIAARYVLAPGIFNA
                     LRRTAPGKGDEIQLTDAIRLLIQEGKKVLGIRLRPRERRFDIGNYDAYFRAFVEFALA
                     DKKYGPALRKFLVQLLHETHP"
     gene            complement(21029..23107)
                     /locus_tag="PKI07_07545"
     CDS             complement(21029..23107)
                     /locus_tag="PKI07_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOC50945.1"
                     /translation="MIATTLARILTKGDRVAVSNVTGREAMGVSIASQQYCGNIVGGW
                     ALGHDDEVIAVPRGRDIRVFGQFADLLKALPARERPNKVVVYSPPDAVYGDVKEVVEN
                     GQGIVKTLFVVTEHVSVEVTAKLRRLCDLARMDIIGCNTLGVINAPAGVRVGAVGGDA
                     PGESFLPGSATIISNSGNLVNTIATYLQSAGLGISFGISTGKDPLILTPLKDFLVLAE
                     KDPRTRIVVLYVEPGGTYERDALELLRRRNYTKPLLVFVAGAFAEGRDISLGHAGGVV
                     DGPCTSATDKMRLFDEYFGSAPFNPADTRAASRHLARTRRGLRATTLHALVPAAKVLM
                     EVLRLKPDFKPVRPVALNPWIVDLGALADKLPASLALQPGNVPSPYKELLAQQLKGGL
                     GRVVARQSMRNASHASSNDGATPRVYGYSLPALMERPAPFAQAVLLAWLGSPPARDFE
                     PRLFEMCLTASLTNGPGSISAQGAKLAASAGNEPHTAMMATLGTIGSIHGGNGKEAAR
                     FLVRLFRETGLVDPYDRKKAPNLDQRVQAYVADYQHRKSAAKNAGVEMEKVPCLGHPV
                     FNREAVNYDPRERVISRYLEAQGLYNVFLDFYHRLARELQRQGVTSKVHAVNVDAVLT
                     CVCLGIAWPLLVEKKITLERAVDLPFLTFALGRVAGGAAEYLDHRESGTDMDMRVPVS
                     ECRELTRPRD"
     gene            complement(23104..24348)
                     /locus_tag="PKI07_07550"
     CDS             complement(23104..24348)
                     /locus_tag="PKI07_07550"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HOC50946.1"
                     /translation="MTNTRIGELVFLEHFAPLYKLPVPEHLAEPVTQSKLKEKLGRWG
                     AGIVKPDVLAGKRGKAGLVRKVENVQDALRVLRDVAAEEINGQNPRGAYVVQMVPADL
                     EIFTAISYSARTLSPAFTVSLRGGMEVEAISEADKVTVPLNVFQGLNAYQASEALAKL
                     GLKGKLNSRLSIVFVNLWDMFITTGMLRCEVNPWRVTTDGRIWACDFKATFDENNFKA
                     RDLGLSWPEYPSRETAFEAEMNAWAAASHQGQAHVSSLGGKKILPLLFGGGASTIITE
                     TLTQLGGEPIFLSDFGGNPPYERMKKTAAICFEHHLHQCALLLILGGKANNTLIDVTF
                     SAIADALQECVDAKGPVSIPVVVGRGGARLVQGMVALSKTLSNLRLPYVFFGPDTPIT
                     LVAEYAVALMRAGEQRKPKGRL"
BASE COUNT         4075 a         8236 c         8207 g         4317 t
ORIGIN      
        1 cccgccaaag cgtttccagc ccggactgtc caattattgg acacttaagc agcctgcacc
       61 cccacccctc gcttcccaac ccgcccaacc acgttttccg cccaatttcc cctgtttata
      121 gccatattat ggctgccaaa tagccgtgta caaggggtgt acaagggatg cccaccgggt
      181 gtacgtcgga aaatttgatg agcatccggt gagcatccct tgtacacccc ttgtacacgg
      241 cgctttggca gggtatttta cccctgtcag gagggtggag gggcggattg actgcttatt
      301 ggagttggaa ggcttcgagg ggcttgaagc tcgcgcgccg ggctggcggg cgttcctgcg
      361 gcgctggaaa tcgccgggac agggtggggg aaaggggggt ggcggggttt tttgaggcgg
      421 gatatttgcg ctgttgcggt agcgggcgtt gcgggccaaa ctgggctggt atggctgtat
      481 tctatgatac tcacgcccat ttggggcgtc gtgagtttgc ggaagacctg ccccaggtca
      541 ttgcgcgggc ggaggcggcg gggattggcc ggattattac cattggcacc gatcaggcca
      601 gcagcgagcg cgcggtggag ctggcggagc ggtatccgca ggtatatgcg gcggtggggt
      661 ggcatccctc ccaggcggcg gaagcgccgg aggattttcg gccggccttg cgcgagctgg
      721 ccgggcaccg caaggtggtc gcgctggggg agacgggact ggattactac cgtctgccgc
      781 gccgcccgga gggggccccg gcgggggcgg aggcccggga caaggcccgg caggcggatt
      841 tgtttcggca gcacctggaa gtggcggctg aaacggggct gggttgcatc attcaccagc
      901 gcagttcgct ggaagatacg ctggccctgg tgcggccgtt tgccggccgg gtgcgcgggg
      961 tgttccactg ctttgccgaa gacgcggcgg ctgggcggcg catcgcggac ctgggatgcc
     1021 tggtcagctt caccgggatc gtgacctata agaacgcgcc gctcgtccgc gaaacgctgg
     1081 cggcgacgcc gctcggccag ttcatgctgg aaaccgactg cccgtttctg gcgcccatgc
     1141 cttaccgggg caaacgctgc gagcccgcct acgtgcggga gatcgccgcg gccgtcgcgc
     1201 aggtgaaagg ctgttcgctc gatgaattaa gcgcggccac ctgcgcggct gccgaaaagt
     1261 tctttgcccg gccggcggcg ggcggatagg ccggacgcct ccgccgacgg catcggcccg
     1321 cgcgggcgtc ccggcgcccg cgggcgagcg cgggaggacg ggagggcgca tggccgccgg
     1381 atccggtttt cccgcttgcg ctccgtgtgc tacggtttgt ggaggtgacg cgcgtgacga
     1441 tttcccggtt gcccggtcaa actctgacga tgatgcgaat ggcgttatag cgggttatga
     1501 agcacgacga atttgccttt ttcaatcagc aactggcggc gatgctccgc gaggatattc
     1561 cgttggaggg ggcgctgcgg cagttgtgcg ccgggatgcg caaggggccg ctgcgcgacg
     1621 agttgcagcg cctggaggcg gacctggccg ggggcacgcc cctgccggac gccctgtccc
     1681 gccgcgccct gccggaactc taccgccgga tggtgacgat cggcgtccgc ggcaacgatc
     1741 tgcccggggt gctgctcttg ctggcggacc attaccaccg ccacaacacg ctttgggcgc
     1801 gcctcaaagg attgctgctg tacccgctga tcgtcctggt cgtggcgctg ggattgacgc
     1861 tggttttaac gctgatcctg aagtggtttc tggcggaaat ccgcggggcg ccagagctgc
     1921 tagagtgggg tggtcgctgg cgcctgtttg gccagtggcc cgtggagatc ctgatttggt
     1981 tcccgcccat cgccctggcg ctgggggcgc tggcgggcgt ggtggcggca accctgccgg
     2041 cttgccgcgc gcggctgcgc tggcggctgc cggcctttcg cgaagccagc ctggcgcaac
     2101 tggcttcggc gatcgccttg atgctcaaga acggcgcgac tttgcccgaa gccctggccc
     2161 tggcggaggc gctcgagagc cataccccgg cggccgctcc ccttgcccgc tggcggcggc
     2221 ggctggaggc cggccagggc aaacccgcgg attgggatgc ggagaatcgc ccctttccgc
     2281 cgttgttcct gtggctggtg cagcggagcg gcgaggacgt ggcggcgggc ttccagcagg
     2341 cggcggatct ctaccgggcg cgcgcttcct atcgcatcga gatgatgctg tacggggcgt
     2401 tgccggttgc catcgtgctg ttgggtctga tggtgctctg gcaagccgcc ccgggggtga
     2461 tctggctgac tcggttaggg gattttttgt acttggtggg ctctttctaa agggatcggt
     2521 tatgagcgtg aacgatgtcc cactgcttct ggccgccctg cttccggacc tgggcgcggg
     2581 gatcatgact ttgctggcct atctggtgat cggcctggcg cccgccgtcg gcattgctta
     2641 cgcgatctac gtgctcttaa ccctgcccct gcggcgcaat gagcgggccc ggatgttcct
     2701 gaacctgctg gagctgggga taaaggaagg gcaggcgccg gaggcggtga tagccagtgc
     2761 gtcggccagc cgcgatcgtt ccctgggggt gcgctttcat tggctggcgg cccatatcga
     2821 acaggggctg cggctcagcg aggcgctgga acgggtgccg cggttgttgc ccccgcaggt
     2881 ctgcgccatg ctgcggatcg gcgagcgcat tggcgaggtg caaaaggttt tccccgcctg
     2941 ccgctggctt ctgcgggaca gtgtctccca ggtgcgcggt gcgcttaatt atctcctgct
     3001 cgcggttttt gcgatcaccc cgggcctgtt catcatcctt ttctggctta aaaaagctct
     3061 cctgccccag ttcatggcca tctatgccag cctggaggag gggatgagcc tgcccgcgtt
     3121 cacgcgcctg atttttaacg acagcccggc gctgctggcg attcagctcg gcgtgctggc
     3181 cattctgtgg ggagtgacgc tggcttacct gggcgggccg cggctgcacg gctggattca
     3241 tcgcctggcg cccggcctgc aggacgggtt tttggcgcgg ctgccgtggc gaaatcaccg
     3301 cctccgccgc gatttttcgg ccatgctggc cctgctgctt gacgccgggg cgccggaggc
     3361 ggaagccgtc cgcctggcgg cggagtccac cgccaaccag accttcatcc gccgcggaca
     3421 gcgggtttgc gcccggttgc agcagggggt cagcctgccc gaggctttgc gcgcgatgga
     3481 caacaccccg gaactgcatt ggcggctggc ccatgcgctg cggcgcgggg cgggttttgt
     3541 ccgggcgttg ggcggctggc acgaggcgct ggacgcgcgg gcgtttcagt gggagcaggc
     3601 ggccgcccag gttctcacca cgggcctggt cctggtcagc ggcctggtgg tgggcggctt
     3661 cgcgatcggc atctttctgg tcatcgtcca aatcatcaac acgggttgtt tatggtaatc
     3721 accgcatccc tggttcacgg gttccgcccg ccccggcgtg ggcggcagcg cggcgcgctg
     3781 atgacggagt tgctcgtggc gctggcgatc ctgatgggga cgctgctgcc gctggcgtat
     3841 gcgtttgccg cggaacaccg gctggcccgc gcctgctacc agcgcgccgt cgcccttgaa
     3901 atcgtggacg gcgaaatgga agtcctgctg gccggcggcg ggcgcgcctt cggccccggc
     3961 acttatgatt atccggttca cgcccgcgcc gccaccaacc tgcccgcggg gcgcttcatc
     4021 ctgaccgtcc agccggggaa gctgcggctg caatggcggc ccgagcccaa aaagcatggc
     4081 ggatccgtcg tccgggaggc cgttttccca tgaaaaccgg ttgcgcatgc cgatccgggg
     4141 cccgccgcgc gggccgggcc gcctacctgc tgatcgaggc gctggtttat attgccgtgc
     4201 tggggggcgt gctcggcgcc ggctacctcg ccctgtatcg gggcctggac cgttcgattg
     4261 cgctgcgccg caacgccgac gaaatcacca gcgctctcca tgccggcgaa cgctggcgcg
     4321 ccgatgtgcg gtcggcaacc gccccgccgc ggctgggaac cacggaggac ggacagtttc
     4381 tttacctgga aaggccggcg cggagcgtcg cctaccgttt cgccaccaac gccgtctttc
     4441 ggcagtccgg cgggggcggg tgggtatgcc tgctgcgcca tgtggagagt tccgtcatgg
     4501 ccgctgaccc gcgcgagcgg gtggttgcct ggcgctggga actggagctg cggccgcgcc
     4561 cgagcggcag cgtcaagccg ggccgcatcc gcccgctgtt caccttcctt gcggttcccg
     4621 aaaaacccgc cgccccatga agctgccttc cgtccatcgc attcgttcca gccgccgcga
     4681 gtccggcgtg gcggtgatcg tcatcctggc tctcctggcc atcctgatga tctacgtggc
     4741 cggcaatctc cgcacgctcg accacctcga acgcgaggtg cggctgctgg agcggaagca
     4801 aatcctccgc ctccaggcaa ccgcccgccc cgcgtcttcc gccgtggaag cgcccggcga
     4861 gagccggggc ctccgccccc cttcccccgt ttccgcaacg gcggagggcg cgccgccggc
     4921 gggagagggg cgggttcggg aagaccacga gcggacgggt tcggagaact cccttcccgc
     4981 cgccccgccg gccgccaatg accctcccca ggtgcgggga accgcagacc catcaacccc
     5041 ggaatccccg ccatgaacgc gctctctcat gacaatcgag atgggaaagc ctccgggcgc
     5101 cggggatggc tgttccggcg gttggtgaat ccggcccgcc cgaaggcgcg ccaaccactc
     5161 acgggacccg cgcttcgcat ctggctgatg cggcggtcgt tggccacgtt tgtctggggg
     5221 atattcagcc tcctgccggt gattggcttt tcccttggcc tgggcgtgct cgcttactgg
     5281 gtttgcatgc ggccttatcg cacccgggaa tggaatccgg cggtggttta tttgcgatgg
     5341 ggaatcggcc tggccctgct tggcgtgact ggctcggcgc tgatcctgta ttactgggcc
     5401 agactggttt tcctgtcggg gcagtactcc atctacaaca acctgtattg atccgctttt
     5461 cgcaggcgat gccggggcgg gggtgaccgc ggccggcccg gccatcggga gccgggcgct
     5521 caacgccatt cgtgccgggg gtatttgcgt tggagttctt gttttatccg ggggtagtgc
     5581 tcccgccaga agccggcgag gtcctgcgtg atttgcaccg ggcgctggct gggggccagg
     5641 atgtgaacca gcacgggcac gcgccccatt gcgatgcggg gtgtggcgtt gaccccgtat
     5701 aactgctgga tgcgcagggc aatgtggggc gggttggcgg cgtcgtagat gactttcggc
     5761 gcgcggccat tggccagggt gagccgttcc ggggcgtgtt tcgacagcag ggcctgctgg
     5821 gcggcgctga gccagccctg cacgagcttc cggacgggct tgtttttgag ttcccggccg
     5881 ctgaaagagc cgtggcagat ttgctcgacg aggtggcggc gttcggcttc gccgaggggc
     5941 ggcagggcga gttccgggca ccattgggcc agccggttga gccggagcac ccattgttcg
     6001 accgcctcgt cccagtcatg gagttccagc cggcccgcca gcacttcgtc ggccagcaac
     6061 cgggcggcgg cctcggccgg gggcggctcg acccggcgcg cgccgatgga caggccgcga
     6121 aagtggagtt gttcctcggc atagacgcgc ttcgcggtcg ggtcataaaa gacgcgcagc
     6181 gccggggcga gggcgtcggg gaagagttcc cgcagccagt cctcttcgat cgccgtggcc
     6241 aggctgagcc gcgtttgcac gctctggtcc gggccttcca cctcgcggac ttccgccgcc
     6301 acgagcagcg ggctttgccg gaccacgctg tcccgggcca gttggccgcg gcggccatgc
     6361 accaattcgc agcggagcgt gccgccatcc aggcggcggg ccacgcggtc gctgaacccg
     6421 agcagaaggc acttttgcag ggcggcgtcc gccacggggc gcggggcagc gtcgaggccc
     6481 tcgcgccggg cgaggtccag gaactgctcg aagagcgggc cgatttgccg ggccgcggcg
     6541 gcgtggacgc ccaagcggcg gcaggtttcg gggtggaaat cccgggcgga gacgtgctgc
     6601 cagacgctcc ggagcagcca gaaatcgctg ccggcgctga cgcccggcag ttcctcgcgg
     6661 cggcgcgcga tctcggcgtc cgcgtggcgc aaaagcaggt cgcggccctg ggtcagggcg
     6721 gccagcaggc aggcctggtg gacgcagcca tattgctgcg ccgccagcaa catgcgggcg
     6781 tagcgcgggt gcagcgggaa ggcgagcatc tttcggccca ggggcgtgag gcgggcgggg
     6841 gcgccggacc ggtgcgggcc gggggccgcc gcgagcgcgc ccaggtcggt gagcaattcc
     6901 tcggcgtggg ccagggcttg ttcctcgggc ggctcgaacc agcggaaggc gcgcaggtct
     6961 tcgacgccgg cggctttcag ggtcaggacg acttcggcca ggtccaggcg cttgacttcg
     7021 ggcagttccc gcgccgggcg gtgcgcatgt tccgcccgcg accataaacg caggcaaagg
     7081 ccgggcgcgg tgcgtccggc gcggcccgcg cgctggtccg cggcggcgtg gctgatcttc
     7141 tcgatcagca gggtgttgag gccgcggtac ggatcatagc ggggcaggcg cgccagcccg
     7201 ctgtcaatca cgcagcggat gccgtcaatg gtcagggatg tttccgccac attggtggcc
     7261 acgacgactt tgcgctggtc gtagcgcgcc acggcggcgt cctgctcgcg cggcggcaat
     7321 tcgccgtgca ggggcagcag ccggaagccc cgcgcctcgg cgcggcgccg gagcgcctcg
     7381 agggtttggc tgatttcgaa gccgcccggc atgaaaatca agacgtcgcc ctccccgccg
     7441 ccgcggacga agccggcaaa ggcttccgcg gcctgctccc aaacggggcg cagatcgagg
     7501 taggcgggcg cggcggcgaa ctcgatgtcc accgggtagg cgcgcccttg cgattcgatg
     7561 acccggcaag gcttgaggta gcgttcgagg ggcgcggttt cgagggtggc ggacatgacg
     7621 aggattttca ggtccggccg cccggtttcc tgcagggcca gggcgcgggc cagcgtgatg
     7681 tcgccataaa gatggcgctc gtgaaactcg tcgaagatga gcgccgccac gccgcgcagt
     7741 tgggggtcgc gaatcatctg ccgcagcagc aggccctcgg tgacgaagcg gatgcgggtg
     7801 cgggcgctgg tgacattttc gaagcggatc tggtagccaa cctcgccgcc caggcgggtc
     7861 cgccgctccc cggcgacccg cgccgccagc agccgcgccg ccagccgccg cggttgcagg
     7921 atgaccacct ggccctcccc caggacgccc cgctccagga gcatctgggg gacctgggtg
     7981 gatttgcccg agccggtggg cgcggacacg atcaggcgtc ccgcggcctg caagccggtc
     8041 aggagcggtt gttcgagttc gtagattggc agccgttcag ccatgggcgc cacaggctac
     8101 acggcccgcg ccgattttgg aagcagggga accggggcga taaatgcctt ggcccggccg
     8161 ggggggcgtg ctaggatacc cggcatggtt catgttggca tcggctatga tgtgcatgcc
     8221 ctggtcgagg gacggaaact catactgggc ggggtggaaa tcccccatcc caagggtctg
     8281 gagggccatt ccgatgccga tgccctgatg cacgcgattt gcgatgccgt gctcggcgcg
     8341 ctgggggagg cggatatcgg ccatttgttc cccaataccg acccgcgctg gaagggcgcg
     8401 cccagccggc ttttcctgaa tgaagcggtg cgcctggtcg ggtttcgcaa cgggaagata
     8461 gtcaatgtgg acgccacggt gatcgcccag gccccccggt tgctgccgca catccgcgaa
     8521 atgaaggcca atatcgccgc cgcgctcgag ctgaacattc agcaagtggg cgtcaaggcg
     8581 accaccaatg agcacctcgg ctttctgggg cgggaggagg gcattgcggc catggccgtg
     8641 gccagcgtgg atttgccatg aacccgccgc tcccgccggc ggcgcggccg cccgttggct
     8701 ccgcctgatt ccccgccatg gccaaggccg agatcagttg gaagcgcacc acggcggagg
     8761 ggattcgcct gcaggtgtat gcccggcatg tgggccgcga atggcgcttc ttcgcgcggg
     8821 aacgccggta tgagctgtgg caggcgcttc ccgagccgcc gctggaggat tggctggccc
     8881 tgctggattc ggtccagcgg cgcatccagc gccgcctgct ccggcccgag gaggaggcgc
     8941 gggtgaagca gtccatccgg gaacgatttc ccgaggccgc cctcgaatga cgacggcagc
     9001 ccgggagcgc ggtttatgtt tttgcctgac gccccggccc gttccggcca gaatcattgc
     9061 ctatgactag accgccgctg atttttattt cgccgagcgt ggagaagaag ggagacgagt
     9121 tcggggacct ctcggtcagc ctgtcggaga cctatcaaca ggccctgacc gacgccgggg
     9181 ggctgccgct ggccctgccc gcgctgccct cgcgggagtg gatcgccgcc agcgtgcggt
     9241 gttgcgacgg ggtcctgctg acgggcggcg acgatgtgga cccgcgcatt tatggccgga
     9301 aactgccgcc cggcctgcag cgcaccgtga ccctcacccc cgatggcggg gcgcgcgact
     9361 ggcgtgaatt ggtgttgatt gacgaggtct tccgccagcg caagccgttg ctggcgattt
     9421 gccggggcct gcaaatcctc aacgtggccc tgggcggcac gctgctggcg gatattgcga
     9481 gccagcggcc cggggcgatc aaccatcgcc gcctcgacca gcgcagcgag attgttcacg
     9541 aagtgctatt gacaccggaa tccctgttat ccaagataac cggtgggcac agtctaggcg
     9601 tgaacagcac tcatcaccag gcggtggcgc gggtggccgg acctttgcgg gtcgatgcca
     9661 ccagccgcga cgggatcatc gaggggctgg agttgaagcc cggcgccgcc cggatgctgc
     9721 cgtttctgtt gggcgtgcag tttcatccgg aacggctggt gaagcgtcac cgcgaacacc
     9781 gggcgatctt tgcggcgttt gtgcaagcct gcgcccggaa cggcaaattt gatttatgaa
     9841 gggcaaaata ctgatcgtag atgacgatgc gggctaccgc gagctgctga agggctttct
     9901 ggagggcgac tatgagttgt ccgaagccga aagcggcgcg acgctcaaga aggccatgga
     9961 gcagggccag ccggatatcg tgctgctgga cgtggagctc aaggacgcca acggcctgga
    10021 gatgctgccc tccctcaaga aacgctggcc cgaaaccgag gttatcgtgc tgaccggcat
    10081 gccccgcagc gacacggaag ccgttgcctg ggcggtcgcg gcgaccaagc ggggcgcctt
    10141 taatttcctg cgcaagacgg agcagttcga tggcgggaaa ctgctgaccg atgtttccaa
    10201 tgccctggag gcccgccgcc agaccgagga aaccagcatg ctgcggcgcg cgctggagac
    10261 catgagcggc agcgcctcgc ccattttcca cagcgccgcc atgcgcgagg tcgtccgcac
    10321 ggtcgagcgc atcgcgccca gcgacgtggc cgtgctcatc accggcgaga gcgggtccgg
    10381 caaggaagtc atcgccgatc tcatccacac cttcagcccg cgcagcaagg gccggatcat
    10441 caagattaat tgcgccgcgc tgccgcggga gctgatcgaa agcgagctgt tcgggtccgt
    10501 caaaggcgcc tacaccggcg cgcataccga ccgcgagggg ctgtttcggc tggcggaggg
    10561 cggcaccctg ttgctggacg aaatctccga gatgcccatt gacacccaaa gcaagctgct
    10621 gcgggtcttg caggaccagg aggtgcgccc cgtgggcggc aaaacggcct acaagaccaa
    10681 ctgccggctg gtcgcggcca cgaaccgcga tccgcgcgag gcgatcaacg acggcaagct
    10741 gcgcgaggac ctgttttatc gcatcagcgc catttccgtt catctcccgc cgctgcgtga
    10801 acggcgcgag gacatcatgc cgctggccaa tgcgtttttg aaacgctttg cggcccaggc
    10861 caaccgggtc atcaaaggct ttacggccag cgcgatcgag cggctcaccg cgttcgactg
    10921 gccgggcaat gtgcggcagt tgcaaaacga ggtgcagcgc gcggtgctcc tgtccgaagg
    10981 caacgaggtg gacgcggccg atttgtccat cagtacggcc cggcccggcg cgggggactc
    11041 gtcggacacc aatttcacgc tgctggaggg agtcgagcgc aacgccatca tccagatgct
    11101 caaggaaacg aacggcaaca aactggagac cgccaagcgc ctgggcatcg gccgccagac
    11161 cctctacaac aaaatcaaag cctacgggat tgatgtctga tggcctcctc cgccgctgct
    11221 tcttcgggac aggcggccaa ggggcgaatc ctcatcgttg atgatgagga gggcatccgc
    11281 gaattgttga aggcactgct gctggaccgt tacctcgttt ccgaggctga ttccggcgcc
    11341 accctgcgcc aggcctttgc ctgcgaccgg ccggatgtag tgctcctgga cctgaaactg
    11401 cccgacgcca atggcctggc cctgctgccg gtcatcaagc agcaatggcc ggcgaccgag
    11461 gtcatcatcc tgaccgggca gccggacgag cacggggttc cgtcatgggc cacggaagcg
    11521 gcccggggcg gggcgttcag cctggtctcg aaatcggcgg agtttgattg ccagaaactg
    11581 ctggacggca tcagcctggc gatgctgcgc agcctgcaag cccggggaga gcacgcgccc
    11641 tctcccggag cctgaccgcc gggccaaaac gccaaaacga tgaacctgaa ccaagcccgg
    11701 ataacggcca cccgggccgc cctggcggcc ggcgacctgc tgcgccgcga actgcacgcg
    11761 cccaaacgcg cccaatctgt tttgcagcat gacatcaagc tggaactgga cgtgcgctgc
    11821 caaaagcaga ttgcccgcgc cctcaaacgc gccttcccgg aggttgccct gcttggcgaa
    11881 gaaggagtgg ccggccgatc ggacgccgcc cgccgctggg tggtggatcc gattgatggc
    11941 acggttaatt tcacctatgg cattccgcat gcatgcgtgt ccatcgcgtt gcagcagcgc
    12001 gccgcgggcc gggctgatta ccgcgacggc tatgatacgc tggtgggcgt ggtctatgat
    12061 cccttctgcc gggagttgtg gaccgccatc cgcggccagc cggcgcgctt gaacgggaag
    12121 atcattcagg cgagccggcg ccgccagttg cgcgaagcga tcgtttccat cggttttgcc
    12181 aagagccggt cgagcctcgt ggcgacgctg ccctacttcg tcaaactggc ccaccgtgtg
    12241 cgcaagattc ggatgatggg ctctgcggcg ctgggtttaa cctatgtggc ctgcggtcgc
    12301 tttgacgcct atatcgagcg cgaaatccaa acctgggaca tcgccgcggg tgggctggtt
    12361 cttcaatgcg cgggcggtga attctggcac gaagccttgt ccgatcccca ccaacaccgg
    12421 gtgatcgcca gcaacgggct gctgcgccac agcctgcgcg ttccgcgttg agcctcgctt
    12481 aagccggagc gacttgaatt cttcggcggc cccccgccgc gtcgcaatcc aaagcgactc
    12541 gtatattcag ttctgctctc tggctggggc gctcccggcg gcggagatcg gccagaggcc
    12601 gttcgggcca aagatttcct ccaggcagcc cgccagccgc cccgtgcgca cggccaggtc
    12661 cagcaccgtg aaccggcggc gcaggcaaaa cgcctgccaa tagctctgcc gcagccgcgc
    12721 gcgggtgatg ccaatctgct ccggttcggt gggcgcgccg gcgtcgtgca gcatctggcg
    12781 cagggtcgcc accggcagca gttgcgcgcg cagccgctcg cgcaatttcg gccagccggc
    12841 tttcagcgct tccaactgct cccgcaattg ctcggggcgg ctgtgcttgg cccgcaattc
    12901 ccgctccgcg acatcgccca gctcattata gcccaactgc tcgcgcgcgt acgccaccca
    12961 cgccgcctca tcccgccatg ccgcgcagca ttgcgccacg tccattttct ccagcggctg
    13021 ggccaggagg cactcatgca gcgcggtggt ggccagggtg ccgatgccaa ccttgaagcc
    13081 atgtgaaggg gcgctgccgt tgtgggtgtg gtgttgcatg tcccacaggt ggctgaactg
    13141 atgttcggcg cccgaggccg ggcggctgga cttggccgcc tgcatcgcaa acccgctcaa
    13201 cagcagtccc tcgaccagct tcccgacggc cgtcgggttc cggctgcgaa cgccggccgg
    13261 gtcggacacc atctcgcgca gccgcccctg caccatcgcc cacgccgacg gatgaatcgg
    13321 ctcgctcccc acgaagtcag ccaaaatcca gtccgcgccg gcgggaacct tggcgacgag
    13381 gtccgcgtat ccccaggcat tcatctggtc cggcgcggcg caaatcacct ccacgtccgc
    13441 caccaccgcg gcgggcgccg ggcactggaa ggtttgcttg gcgccctcgt aagtaatcga
    13501 ggctccgaaa gcggtgtagc cgtccatcga agccgccgtg gcgacgctca tgtaggagcg
    13561 tcccgtccgg tgggaggcca gcttggtcag gtcgttaatc gtgcccgaac ccaccgcgac
    13621 cgggacggcc gcatggccgc gcagagacgc ttccagttcc gccacaaaac ggtgctcggc
    13681 atacaaatcc ggcgcgcgaa agataaacgg ctcgagctgc ggatgccccg tggcgccaaa
    13741 cgcttcggcc accgcctgcc ccgccgcccg gaaggtgttc tcgtctgcca cgatgaccgc
    13801 cgtttgccgg ccaaactgtt gttgaaagac ccgcggcgcg gcgcttcgga ccccgacgcc
    13861 aatttccagg tggcgcgtct cgctcgctgc cgccagcgcc tcagccaagg tgagagtttt
    13921 catgcttgtc caggtatagt atccagcaac gctgctaacg ggtatcgttg ccgttccggc
    13981 gtgtcaaggg catacgccgc aaaaacgcgg ccgccacagg gctttcgata aggaatgcca
    14041 tgccgaacct cactcgcgcc ggcgcagatg attccgaacg tgccgcccgc ccactttgcc
    14101 agcctgcccc ttttaggtta tctgcgcccg caggagattc gggctgccgc gaccgtggtt
    14161 cctacggtcc cgtcaggtgg aagttgccgc ttacgttgaa agggggatcg ctgtagaaag
    14221 tgcccttgaa ggtgccgttc gtattgctgg taaacgccag attggcggtc agcggaacca
    14281 agccggagaa ataaatgggc cagagaaagc cgcgataagg ctcctgggtc cagctatact
    14341 ccgtcaccgt ccccggatcg aagggcggaa tggtatattc tcctccgccg gccgaatcaa
    14401 aggcgatagt caaggtgttc gtcaaggcag aggtaaaagt aagcgtaagc gtgcggccat
    14461 acattgtctt gggcgcaaag gtggaggagg gatactgaac ctgggccaag cggaaaaact
    14521 tgcgcggcgc gtcgttcgcc tgcaggacgc ggttggtaac cggcgccgcg gtttcgattc
    14581 ccagcgcgtt cgccgtccac cctcccgcta gattggccgt cccgaaaagc tggttgtcgg
    14641 catactgccg gttggaaaag gtgaggctga cctggccggg gagcgcggcg attcccaacg
    14701 gcagattggt cacgatcggc aggccctggg cgtggatgtt gaaggcttgc gcggcggtcc
    14761 ccacccggcg gatgctgacc tgctggatga tcaccggagt aagcggggcg tcgttcgcgc
    14821 cggtgggcac ctggttgatg gcataaacca cgttggaccc cgacaccacc cgtccaaaga
    14881 tgctgtaggt attattcccc gcggggaacg gcgcgacggt gataaagaac tgcgccccat
    14941 cggaattggg gccggaattg gccatcgcca gcactccgaa acggtcaaag accagcttgg
    15001 ggctgaactc atttttcaac gcgtagccgg ggccgtcggt gcccatcccg ttgggcgagc
    15061 cgccctggtt cataaaaccg gcgatgatgc gatggaacgt caagccgttg tagaacgcgt
    15121 tggtgcgggc gcggcccgtt gtcaggtcaa gccaggcgcg ctgcccggtg gccagcccga
    15181 tgaagttggc ggtcatgcgg ggcgcgttgg tgtaatccag ttggcaggtg aaattaccct
    15241 tgctcgtgac gaaatccgcg aagattccat ttccctggcc catcgcaatt cccggggaca
    15301 ggcccaggat cacgggcagc gccagccgcg cgacgattcg atgcggagac atccggttca
    15361 ttatttgctc gagttcagta cgactacatt cgccatgcct tcgcggagac gccgatgact
    15421 ggagccgggc gccacgcaac cgggcaggct tcggggaaag gtatcagtcc cggggggcgg
    15481 ggctcaatct cttaattttg cgcccagcaa ggccagcgcc cggctgcgat ggctcatccg
    15541 gttctttatc gcttcgccca gttcggcaaa ggtcgcttcc tggcccgccg ggataaacag
    15601 gggatcatac ccaaagccgc cttgcccgcg cggcgcaagg ccaatccgcc cctcgcaaac
    15661 gccttcaaac aactccgtcc gcagcatcat ctccccggcg cgccccggcg ccgcggcggc
    15721 tcccgccggg aagcggggca ccggggtcag cgccagcaca catcggaacc gcgccgttcg
    15781 ctccaccggc ggaacttccg ccagcaggcg cagcagcttg gcgttattgt ccgccgccga
    15841 cgaattgccc ggatggcccg tgtccaacgc cgcgaaccgc gcggagtgaa cccccggcgc
    15901 gccctgcaaa gcgtccactt ccaggccgga atcgtccgcc aacacatacc attcgcccgt
    15961 ttccgtgccg ggcttcacgt tcggcctttc cgccagccat cgcgccagcg cttcggcctt
    16021 ccgcgccgcg ttccccgcaa aggtctcggc gtcttcaacc acctcgggcg ccccgggaaa
    16081 gtcatcgagc gtcaggcagc ggacgccttc tcccaaaatc gcccgaatct ctccgacctt
    16141 gtgcgcattc cgtgttgcga taagcaagct gaccatttcc gccaagccta acccgggacc
    16201 gccccggaat aaagcccggg ccttgcccgc gccgccccgg aaaccgcgcg catttccggg
    16261 cgccaccgtc ccggccgccg ctccgctcgc gcgaagccgg gcaggggagc cgcctcccgc
    16321 ggcgactggt tttgcggcgg gggcatcttc ttgcttgccg ccgggggcgg cttcgataca
    16381 ctccggcgcg tgcacacgtt tagaaaacga gcccggttct gctgacgcag cgattcactg
    16441 aatacgatta accacaacct taaagattta tgccaaaacc cattactgtt tatttgccct
    16501 acagcggcca ggaacacacc cgccggaccc ttgaccagtt gcagcaatcg ccggtaatcg
    16561 agcgcatctg cctgctgacc gccggcagcg gcggcaaact tcccgccggt tgtgatcggc
    16621 tggccgtggg ggctctgcac gccagccgga ccatgcgcat gatcgcgcaa aacacccgga
    16681 cgccctacac gctgctgctc atccacgaca ccacgatcga gttttggcaa tatgcgctcg
    16741 aacgcatgct cacggtggcc gggttgacgg gcgccggcct ggtttattca gactaccacg
    16801 acatccaggg cggccagcgc gtttcgcacc cggtgattga ttaccaggcc gggagcattc
    16861 gcgacgattt caacttcggc tccgtcctgc tcttcgattc caaagcgttc aaggcggcgg
    16921 cccgccagcg ccagaacttt cagcacgccg ggctgtattc gctgtggctg ggcgtctcgc
    16981 ggctccgccc gctcacccgc gtcgccgagc acctctacgg caaggtcgaa ctggacgtcc
    17041 gcaaatccga ccagaaacag tttgactacg tggacccgaa gaaccgcgcc gtccaaatcg
    17101 aaatggaaca ggcggccacg gagcacctga agcgggtggg cgcctggctc aagccccgct
    17161 tcaagactcc gaacctggac cgggtggcct tcgtccatga agcctccgtc atcatccccg
    17221 tcaagaaccg cgtgaagacg gtcggcgacg ccgtccgctc ggtcctgatg cagcaggccc
    17281 ccttctcctt taacctgatc gtcgtggaca atcattcgac ggacggcacc accgacctgc
    17341 tccgcggcat cgcccagaag gatccccgca tggtgcatca cattcccgag cggcaggacc
    17401 tgctcatcgg cggctgctgg aacgaggcga tcatgcaccc ggcctgcggg cggttcgccg
    17461 tccaactgga tagcgatgac atctacaagg acgaaacgac gttgcgccag atcgtggaag
    17521 cgttccaccg cgaaaagtgc gccgcggtga tcggctccta ccggatgacc aacttcaacc
    17581 tggaggagat tccgccgggc gtcattgacc accgggaatg gacgccggac aatggccgga
    17641 acaacgcgct gcgcatccac ggcctgggcg ccccgcgcgc ctactacacg ccgattatcc
    17701 gcgagattcg gttcccgaac gtcagctatg gcgaagatta tgccgcggtg ctggcggtca
    17761 gccgggaata ccaaatcgcg cgcatctacg agccgattta cctctgccgc cgctgggagg
    17821 ggaactccga cgcgggcatt gatgtggccc ggcagaatgc gtttaacttc tacaaggaca
    17881 agctccgcac cttcgagctg atcgcccggc agcggctcaa ccaggccgcc cagccgccgg
    17941 tccggccggc ccgcaaggcg cccgcgcgca aacgcaaaac cggcaaagcc cgccgctaac
    18001 ccgcccgccc cgccgtgacc cgatccctcg aacaaatcgt catctcaccg gccgaattgg
    18061 cgcggtttgg caccggaacc accctggccg accaggcgca tgccctgctg gcgcagcaga
    18121 agggcgtatg ggaaatgctg cgcaatggtt atgacaccct gcgcaccgtg caaacccggg
    18181 tgttcgactt cgaccggttc cacatcaagg tccagttcaa cccgggccgg atgacttcga
    18241 ccgtggccaa ggtggacgcc acctcgatca aggagcgcaa gtgcttcctg tgcctggaaa
    18301 acctcccgcc cgcgcaacgc ggcctcgcgt gcgaagcgga ctacctcgtg ttgtgcaacc
    18361 cgtttcccat tttcccggag cacttcacca tttcctgcct gcggcacacg ccccagttga
    18421 ttcgccactc gctggccgcc ttcctcaaaa tcacccgcga cctcggcgcc cgctacctgg
    18481 tgctctacaa cgggccccgg tgcggcgcct ccgccccgga ccacctccac ttccaggcgg
    18541 ggaaccggtc gtcgctgccg ctcgatgccg agttcgaggg catgaaggcg gcctgcgcga
    18601 cccggctctt cgagtccgcc gcggtgcgcg tgtggagcgt cgagaactgc ctgcggcatt
    18661 tcttcacctt cgagtccgcc aatgcgggcg ctttggagca gtcgttcgcg gcgctctacg
    18721 cggtcctgca ggaaggcggc ccggcggacg aggagcccat gctgaacatc ctcggtttcc
    18781 atacccggga cgagtggcgg atccatgtct ttccccgggc caaacaccgg ccctccttct
    18841 atttcctgga gggcgaggag aagctgctga tcagccccgc cgccgtcgaa ctgggcggca
    18901 tctgcaccac gccgcgcgag gcggatttcg ccagggtcac ccgcgagcac attgtggagc
    18961 tataccggga agtcggcgtc gcgcccgagc ggttcgccga cctgaagacc cggctggcgg
    19021 cccggctggc ggcggcgccg cccatcggct cctgaccctt cccgggcggc ctcagggcag
    19081 aacctgcggc ttgagggtgc ggcactgcag ggcgcccagg cgctcgctca accgcttgaa
    19141 catggccgcg tcctggtaaa tccccacaat ggccccgccc gacccggaaa actgcgcgct
    19201 ggcgccgcag cggcgcgccg tttcgaccat ctcgacctgc cagggcggca gattgtagat
    19261 gctgcgccgg gtatcgaagt tctcgttcat caggcggccc agctcatcgt aatcgcggcg
    19321 caacagcgcc tcccgccccc gggcggccag gtcggcgcag cgctgcatgg cctggacaac
    19381 ccgggcctcg ccgcggttga aacggccgcg gatgtcgttg tggaacacct cggtcggctc
    19441 gctgaaatcc gtgtggtagg ccagatacag cggcggcagc agggccggat ccagcggctc
    19501 gtagtaatag ctggggaagc cctgcacaac gcgctcgcgc gccgggttga agtccatgta
    19561 caccacccct tcgtaaacct gcgccacgcg atcctgcaag ccggcggtga tgcccagttc
    19621 ttcgcgctcg atcggcagcg cgaagctcgg ctggacttcc cgggggattt cgacgtggta
    19681 gaactccatc aggcaccgca gcatggcaat cacgatgccg ctggaaccgg ccatgcccac
    19741 ctgccgcggg acattggtct ggtagcgcac ggagaagttc tggtcgtgga gttgaatgtt
    19801 ccgggcctgg caataatcca caaaccgctt gatggtggcc ttgatcagcc gaatgccgcc
    19861 gtaatacccg tgcagcttga catcccgggc caggtcctgg atcgaggcga accgcgcccg
    19921 gtcgttctcc gccagcacga tgtccagcgt gtcccactca tagagcacca cctcggcgca
    19981 gaaattgcgg acgatcatgg cgatggtctt gccgtggtag ccgtccgagg ggttgcccac
    20041 cagcccggcc cgcgaatagg cgcgtttacg gatgagtttc atgcagcaat tgcacgagga
    20101 atttgcggag cgccggcccg tattttttgt ccgccagcgc gaactcgaca aaggcgcgga
    20161 agtaagcgtc gtagttcccg atgtcaaagc gccgctcccg cgggcgcagc cggatgccca
    20221 gcaccttttt cccctcctga atgagcagcc ggatggcgtc ggtcagttga atctcgtcgc
    20281 ccttgccggg cgcggtgcgc cgcagcgcgt tgaaaatgcc gggggccagg acataccggg
    20341 cggcaatcgc caggttgctg ggcgcctctt tggcgctggg cttctcgatg acatccgcca
    20401 gttcgaacac ttcccccccg ccccggggct gggccactcc gtagttgtga atctcccgcg
    20461 cgggcacctc ctcgaaggcg accaccgcct cggccttttg ctccataaaa cagcgcgtca
    20521 tgcgccgcac aatatcgctc tgcgcctgca ggccgatgat ggaatcgccc agcgccacca
    20581 caaacggcag gtcgcccaca aagggctggg cgcacaacac cgcatggccc agccccagca
    20641 actgccgctg gcgcgtgtag aaatactgca ccggcgcgcg ctcgaactcc agttcctcca
    20701 gcaactcctc cttgcccgtc tcccgcaggt tctggatcat ttcctcgttc aggtcaaaat
    20761 ggttctcgat ggaggacttg cccggcccgg tcacgaacag cagccgctgc atcccggcgc
    20821 gggtgagttc ctccaccacg tattgcaccc cgggcttgcg gcccaccggc agcatctctt
    20881 tgggttgcga tttggtggcg ggcaacaggc gcgtgcccag cccggccacc ggcactacgg
    20941 cgatattgac ttcattcatg cactgcccgc catgctaccc cgccgccgcg ccggcgcaag
    21001 acgcgaatcc gcccgggccg cggccccctc agtcgcgcgg ccgggtcagc tcccggcatt
    21061 cgctgacggg cacgcgcata tccatgtcgg tgcccgactc gcggtggtcg aggtattcgg
    21121 ccgcgccgcc agccacgcgg cccagggcga acgtgagaaa cggcaggtcc accgcgcgct
    21181 ccagggtgat cttcttctcc accagcagcg gccaggcgat gcccaggcag acgcaggtga
    21241 ggacggcgtc cacgttcacg gcgtggacct tggaagtcac gccctggcgc tgcagctcgc
    21301 gcgcgaggcg gtgatagaag tccaggaaga cattgtagag cccctgcgcc tccaggtacc
    21361 ggctgatcac gcgctcgcgc gggtcatagt tgaccgcctc ccggttgaac accgggtgcc
    21421 ccaggcacgg caccttttcc atctccaccc cggcattctt cgccgccgac ttccggtgct
    21481 ggtagtccgc cacatacgcc tgcacccgtt gatcgaggtt cggcgctttc ttccggtcat
    21541 acgggtccac caggcccgtc tcgcggaaga ggcgcaccag gaagcgggcg gcctccttgc
    21601 cgttgccgcc atgaatggag ccaatggtgc ccagcgtcgc catcatcgcc gtgtgcggct
    21661 cattgcccgc cgacgccgcg agcttggcgc cctgggccga aatcgagccc ggcccgttgg
    21721 tgagcgaggc ggtgaggcac atctcgaaca gccgcggctc aaaatcgcgc gctggcggcg
    21781 agccgagcca ggccagcagg acggcctgcg cgaacggcgc cgggcgctcc atcagcgcgg
    21841 gcagcgaata gccatagacc cgcggcgtgg cgccgtcgtt ggaggaggcg tgcgaggcgt
    21901 tccgcatgga ctggcgcgcc accacccgcc ccagcccgcc tttgagctgc tgggccagca
    21961 attctttgta cgggctgggc acgttgcccg gctgcaaggc cagcgacgcg ggcagcttgt
    22021 cggccagggc gcccaggtcc acgatccagg gattgagcgc caccggccgg acgggcttga
    22081 agtccggttt gaggcgcaac acctccatca aaaccttggc cgccggcacc agcgcgtgca
    22141 gggtggtcgc ccgcaacccc cgccgcgtgc gcgccaggtg ccggctggcg gcccgggtat
    22201 cggcggggtt gaacggagcc gacccgaaat actcgtcaaa caggcgcatt ttatccgtgg
    22261 ccgacgtgca ggggccatcc acgactcccc ccgcgtgccc cagcgagatg tcgcgcccct
    22321 cggcaaacgc cccggccacg aacaccagca gcggcttcgt gtagttgcgc cggcgcagga
    22381 gctcgagggc gtcccgctca tacgtgccgc cgggctcgac gtacagaacc acgatccgcg
    22441 tgcgcgggtc tttctcggcc aggaccagaa aatccttcag cggcgtcagg atgagcgggt
    22501 ccttgcccgt cgaaatgcca aacgaaatcc ccagcccggc cgattggaga tacgtggcga
    22561 tcgtgttcac caggttgccg gagttcgaga tgatcgtcgc gctgcccggc aggaaggatt
    22621 cgcccggcgc gtcgcccccc accgcgccca cccggacgcc ggcgggggcg ttaatgacgc
    22681 ccagggtatt gcacccgata atgtccatgc gggccaggtc gcacagccgc cggagcttgg
    22741 ccgtcacctc gaccgataca tgctcggtga ccacaaagag ggtcttgacg atgccctggc
    22801 cattctcgac cacttccttg acgtcgccat aaacggcatc cggaggcgaa tagacgacca
    22861 ccttgttggg ccgttcccgg gcgggcagcg ccttcaggag gtcggcgaac tggccaaaca
    22921 cgcggatatc ccgcccgcgg ggcacggcga tcacttcgtc atcatggcct aatgcccacc
    22981 cgccgacgat gttgccgcaa tactgctgcg aggcaatgct gacccccatc gcctcccggc
    23041 cggtcacatt ggaaacggcc acgcggtcgc cttttgtcaa aatacgggca agagtggttg
    23101 cgatcataac cggcctttgg gtttgcgttg ttccccggcg cgcatcaggg ccacggcata
    23161 ttcggccacc agcgtaatcg gggtgtccgg accaaagaac acatagggca ggcgcaggtt
    23221 ggacagggtt ttgctgagcg ccaccatccc ctgcaccagg cgggcgccgc cgcgccccac
    23281 caccaccgga atcgagaccg ggcccttggc gtccacacat tcctggagcg cgtccgcgat
    23341 ggcggaaaac gtcacatcaa tcagggtgtt attggccttg ccgcccagga tcagcagcaa
    23401 ggcgcattga tgcagatggt gctcgaagca gatggcggcg gtcttcttca tgcgctcata
    23461 cggcgggttg ccgccaaagt ccgacaggaa aatcggctcg ccgcccaact gggtcagggt
    23521 ctccgtgatg atcgtgctgg cgccgccgcc aaacagcagc ggcagaatct ttttgccgcc
    23581 cagcgaggaa acgtgggcct ggccctggtg gctggccgcc gcccacgcat tcatctccgc
    23641 ctcgaacgcc gtttcccgcg acggatattc gggccagctc aacccgagat cccgcgcctt
    23701 gaaattgttt tcgtcgaacg tggccttgaa atcgcacgcc caaatccggc cgtccgtggt
    23761 cacccgccac gggttgacct cgcagcgcaa catgccggtg gtgataaaca tgtcccacag
    23821 gttcacaaac acgatgctga ggcgcgagtt gagcttgccc ttcagcccca gcttcgccag
    23881 cgcctccgaa gcctggtagg cgttcagccc ctgaaagacg ttcagcggca cggtcacctt
    23941 gtcggcttcg gaaatggcct cgacctccat ccccccgcgc agcgacacgg tgaacgcggg
    24001 cgaaagcgtg cgcgcgctgt agctgatcgc cgtgaaaatc tccaggtccg ccggcaccat
    24061 ctgcaccacg tacgccccgc gcgggttctg cccgttaatc tcctcggcgg ccacatcccg
    24121 cagcacccgc agggcgtcct gaacattctc caccttgcgc accaggccgg ccttgccgcg
    24181 tttgcccgcc aggacatcgg gcttgacaat gcccgccccc cagcgcccca gcttctcctt
    24241 cagcttcgat tgcgtcaccg gctccgcaag atgctccggc accgggagct tgtagagggg
    24301 cgcgaaatgc tccagaaaga ccaactcacc gattcgcgta tttgtcatgc tatggctgcc
    24361 gaccaattgt ttaacgacac ccaacctgaa cccgagatat actcaagctc gcattttccg
    24421 cgccagcttg accttaatca gctcccgtgt caatcaatct ccggttcccc ggcagcttcg
    24481 aggagtctcc cggattttac cggccgtcag ccgctcgctt agcccaaatc ccgggacccc
    24541 ctccggcctg ggtgcccagc caggtcattt caccccacgc gaacccgccg cggggacagc
    24601 ttggccggtg ataccccgca gcccctgaaa tcggccagga tgtccctgcg ccgatcggat
    24661 gttttttcat gcgggatgtg aaacggttcc tccatgattt cgggggaata ggtcaggaag
    24721 atgagccggc ttggggcatg acgccggaag ccttatttcc tgaactgttg ggattgtgat
    24781 taagctggga aagggccgcg agccggtttg agcctgccag cggcgcggtg tttct
//
LOCUS       DAOPQG010000051        24676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2562_length_24676_cov_14.892287, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000051 DAOPQG010000000
VERSION     DAOPQG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 24676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24676
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2562_length_24676_cov_14.892287"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            718..2889
                     /gene="ppk1"
                     /locus_tag="PKI07_07555"
     CDS             718..2889
                     /gene="ppk1"
                     /locus_tag="PKI07_07555"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOC50947.1"
                     /translation="MNANAQAKTFGPADFLNRELSWLEFNHRVLEEALDAQNPLLERV
                     KFFCIVSSNLDEFFEVRVASLKQQVESVVVERSVDGLTASETFRAIVKRVRRMVAEQY
                     ACWREELAPALTASGIRLLELAELEPADRAWVREYYRTQAHPVLTPLAVDLTHPFPQV
                     LNKSLNLIVRLVKAQNGAEREHMAVVQIPAILPRVVRLPRADGRQDYVYLDRLIGHYL
                     ADLFPGTRLLGYWPFRVTRNSELYIDQEETANLLRAVENELHKRRKEDAVRLEIDAHC
                     PAAIRETLLRTLRLTEEDLYLVDGPLNPTRLMALAEGDHSPELRDPPFVAPVAAGLRR
                     RTDLFAAIRERDLLVHHPYDSFDSVVEFLQQAAADPDVLAIKQTLYRTGGDPRIIGAL
                     ESAVRHNKQVTAVVELRARFDEANNIHWARQLDEAGVHVVYGLVGYKIHAKISLVVRR
                     EGLNIRRYVHLATGNYNPTTAKIYTDLGLFTCRPDFGEDATNVFNLLTGICQFQAQRK
                     LVVAPFDLHERLLALVEREARHARQGLPARIILKMNSLAEPQAIAALYRASQAGVKID
                     LIVRGICCLRPGVPGLSENITVRSIVDRFLEHSRIFYFENACQPQVFISSADWLPRNF
                     FRRIELAIPVEDGVLRERLIHEVLAVSLADTVKARALRADGSYHRVRMASGAAAVRSQ
                     ARFIALAAGEDPAARPSAKGKPRYPRVTLAPPPFAPRRQKK"
     gene            2948..4138
                     /locus_tag="PKI07_07560"
     CDS             2948..4138
                     /locus_tag="PKI07_07560"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOC50948.1"
                     /translation="MNLRSRQSTRGARLMVGLMSGTSADGIDAVVAELSGGGRRLRAR
                     VRAHVHQPFRPALQRRILAACQQGTVAELCELNFLLGEHFARAALRAVRRAQLRPADI
                     AAIGSHGQTVHHLPNARTPSTLQIGEAAVIAERTGIATIADFRVRDMAAGGQGAPLVP
                     YADWALFTDERRPRIVQNLGGIGNLTFLPPRARLGDVMAFDTGPGNMVMDGLMTELTR
                     GRQTFDRDGRWAARGQVSEELLAELMAHPFLQRRPPKTTGREEFGEAFLRRLLGAARQ
                     RRLRPEDLLATATAFTAASVAAAYRRFVFPRLTDRERGRLQIILGGGGARNPTLRSML
                     AQRIGAGELLTHEDFGVANAAKEALAFAILAYETWRGKPGNVPPATGARRAVVLGKIV
                     PGAA"
     gene            4324..5424
                     /locus_tag="PKI07_07565"
     CDS             4324..5424
                     /locus_tag="PKI07_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOC50949.1"
                     /translation="MAILNIKPEGSGKYDLIALGEVMLRLDPGEGRVRTARQFKVWEG
                     GGEYNVARGLRRCFGLRTAICTAFADNDVGRLLEDFILQGGVGTEFIKWVPFDGVGRA
                     VRNGLNFTERGFGVRGAVGISDRGHSAASQLRPGDFDWDGLFGRLGVRWLHTGGIFAA
                     LSDTTAELVLEGVQAARRHGVIVSYDLNYRPSLWKSSGGLQRAREVNRRIARNVDVML
                     GNEEDFTACLGFEVAGADEHLCRLEAAAFQQMIEEAVKAYPNFRVVATTLRAVKTATR
                     NDWGAICWADGRFYQAPNREDLEILDRVGGGDSFASGLIYGLLKFNDPQRAVAYGAAH
                     GALAMTTPGDTSMASLPEVEALVKGGSARVQR"
     gene            complement(5459..7084)
                     /locus_tag="PKI07_07570"
     CDS             complement(5459..7084)
                     /locus_tag="PKI07_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HOC50950.1"
                     /translation="MMRNWISNGPRRVVFAWMLAAALPAAPAADRMELVPKWNRCERA
                     FKSSVAYAHPVQDVSLKVTFTSPLGETAEVDAFWDGGKTWRVRFAPDQPGPWRFRTAC
                     SDPTNPGLHDQAGEFLCTAATGATPWHRHGPVRVARDRRHFEHTDGTPFFWLADTVWD
                     GARLAESRDWDYYASTRARQDFTVVQWAAAPGEDARAQSAWGSSPERITINPAFFQRL
                     DAKITALAQAGLVSAIVPLWEQPWPTNAVPLPDDQAECLVRYMVARWSAEPVVWLLAI
                     KSDAATHGLERWKRIGQTVFGDRPHAPVVLFPGRTHWLFDEFRDQEWVDAFGYQNVTD
                     VTDDALMWAVSGPFTAEWQRAPARPLIVFAPHESSIAPRSARRFTADDVRRAAYWGLL
                     LAPPAGVSYGAEELIPWAGIDGKPKRGGGTLWREALTLSGAKQMSHLATFMNSLNYEA
                     LRPQTHRVAAQPGRRSPQRFIAAASTEANTLTVVYVPEDRSVQLRPDALPASPAASWF
                     NPRTGSRQAAVARGGRFTTPAPGDWLLLIQARR"
     gene            7250..8704
                     /gene="gnd"
                     /locus_tag="PKI07_07575"
     CDS             7250..8704
                     /gene="gnd"
                     /locus_tag="PKI07_07575"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOC50951.1"
                     /translation="MEPRADIAVIGLAVMGQNLILNMNDRGFTVAAYNRTVSKVDDFL
                     NQEARGTKVIGVRSIAEMVSVLKKPRRVMLMVKAGKAVDEFIDQVIPHLEAGDIIIDG
                     GNSLFQDTIRRTRYVESRGLLYIGTGVSGGEEGARRGPSIMPGGSPAAWPHVKDIFQQ
                     IAARVDDGTPCCDWVGENGAGHYVKMVHNGIEYGDMQLICEAYHLMKAGLGLGADELH
                     RVFAEWYQGELNSYLIEITRDIFGVKDADGQPLVDKILDTAGQKGTGKWTVISSQDLG
                     IPITLIAEAVYARCISALKDDRVAAARKLKGPRPAIKGDAKRLVEDIRRALYASKIIS
                     YAQGYLLMRAAAREYNWNLNYGGIALMWRGGCIIRSVFLGKIKEAFDRKPDLTNLLLD
                     PFFRQAIKDCQRSWRKVVSLAARKGIPVPAFSTALAFYDSYRSAWLPANLLQAQRDYF
                     GAHTYERVDQPRGQFFHTNWTGRGGTTASSTYTV"
     gene            complement(8721..9281)
                     /locus_tag="PKI07_07580"
     CDS             complement(8721..9281)
                     /locus_tag="PKI07_07580"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC50952.1"
                     /translation="MSDGRTLAGWRETPFTGRGEIQSQPGAIILHMGNPFTGINWTND
                     FPQRNYEIALDAMRVTGSDFFCGLTVPVGTNCCSLIVGGWGGSLVGLSCVDGLDAAEN
                     ETTKFRNFESGRWYRIRLRVTEQRITAWIDEEKLVDLATAGRKFSVRPGDIELSQPLG
                     LAAWQTTTALREIKFRRLDAAADKED"
     gene            9567..9866
                     /locus_tag="PKI07_07585"
     CDS             9567..9866
                     /locus_tag="PKI07_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOC50953.1"
                     /translation="MNLPAFLRAQPDGVLLSVKVQPRASANEIGEALGNELRVKVTAP
                     PVEAAANEALLKLLAQQLRCPRNRVDIVRGHTSRHKTVKLYGLSPEKVLAQLGQA"
     gene            10332..11012
                     /locus_tag="PKI07_07590"
     CDS             10332..11012
                     /locus_tag="PKI07_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC50954.1"
                     /translation="MSTKSGKTKKVTVLLADDHIVVRQGLRALLEAEGDIEVVGEAKD
                     GREAVSLARQTQPEVVLMDVAMPELNGLEATRQIVRELPGTKVLALSSYGNDDYVTQL
                     LEAGGVGYLVKQTAAADLLKAIRQVRKGQVFFSPMIAQRLQAGRPGTPGGGRPGNKHA
                     SLSSREAEVLQLVAEGFANKQIAAKLSISIKTVEKHRQQAMNKLNIHDTAGLTRYAIS
                     RGWVESGV"
     gene            11039..12280
                     /locus_tag="PKI07_07595"
     CDS             11039..12280
                     /locus_tag="PKI07_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HOC50955.1"
                     /translation="MFSEQIELRGHIIDSLLLPKVLDEILTRGGNFTIREIQVGKQRD
                     DASYARIEVYGPSPEVVDDIVRHVRQQGAEVVEQESVRLVEAPADGVFPPDFYVTTNQ
                     KTFVHYRGQWLEVRPVTMDCAIAVDEQRLQAEAVKFAQVQKGRLIAVGHHGIRVAPRE
                     RPGERRSVFEFMGSSVSSEKPKSAIVREVAAEMRRARESGGRILVVAGPAIVHTGAGE
                     HFEKLIAQGYVNRLFAGNALAVHDIENALFGTSLGVYLERGSLAEKGHENHMRAINAI
                     RGAGGIAAAVAGGILRRGIFYQCVKHNVDFVLAGSIRDDGPLPEVITDTLAAQKLMRA
                     RLEGVTMVLMMCTMLHSIAVGNLLPAHVKTICVDINPAVVTKLSDRGTFQAVGLVTDI
                     EPFLRELTEFLAAGESPAGPV"
     gene            complement(12277..13599)
                     /locus_tag="PKI07_07600"
     CDS             complement(12277..13599)
                     /locus_tag="PKI07_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOC50956.1"
                     /translation="MRWRVIAWVSLGVNVLLAAAWWSVIRENAAQRATASALAAEQPP
                     PPPARTNIILRRQLFYWRDIESPNYTNYIANLRDIGCPEQTIRDIIIADVNAVYARKR
                     ATELVTGEQQWWRSEPDLNVLRAAAQKAQELEEERRALLTSLLGNSWETGDLVSLPRP
                     SRLSVTLDGPVLGNLPDETKKNIQEINARSQERLQAYLDAQSRQGLSPDPRELAKLRQ
                     QTRADLAHLLAPPELEEYLLRYSQNANDLRAQFGQLRLFDPSPDEFRAVFRATDALDQ
                     QLHLLADARDPASLATRKALEDQRENAIKIALGPKRYEEYRLLHDPLYQEKLEDADGD
                     PEVARALYASYLAAYHAAETNRLTATQQRALEDQLVPPDLPPLPPAPQPRRVYVLGPG
                     DSAATIALMYGLPESAIRAANPRVDFHRLKPGTPITIPRSPLFPESSP"
     gene            13746..15011
                     /locus_tag="PKI07_07605"
     CDS             13746..15011
                     /locus_tag="PKI07_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC50957.1"
                     /translation="MTTTASTREAGGATRAQWLVLAAAFLGWMFDGVEMGIFPLVARP
                     ALQSMLPVSTPGQDALVGLWMGRITALFLVGAAFGGLGFGWLGDRVGRVRAMVLSILT
                     YSIFTGLCFFARAPWELGALRFIAAFGMGGEWSLGVALVMEAWPAPRRALLAGIIGTA
                     SNAGYLLIALVGLCFQVTRESWRWIMIAGAAPAFLALGIQWVVPESERWKAAMQQGAA
                     RPLREILSAGLLGKTLLGVALAAVALIGTWGSVQWLPLWADQMTGGQNPAAKAVAQIL
                     VSAGAILGSLAGALVAGRLGRRPAYFVLSLLSLLLCGGLFRGVNHYGAVFWVMVTLVG
                     AATAAFYGWLPLYLPELFPTRARATGQGVCYNAGRLLAAAGALTQGQLVAAYGGSYAR
                     AGATITLIYLLGLGLIWLGPETRDQPLPE"
     gene            15110..16153
                     /gene="purM"
                     /locus_tag="PKI07_07610"
     CDS             15110..16153
                     /gene="purM"
                     /locus_tag="PKI07_07610"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOC50958.1"
                     /translation="MKSKAYARAGVDIDLGNRVKADLPRMLQATHRPGVLGKIGGFGG
                     LFELEVRKYRQPVLVSSVDGVGTKLKLAFALDRHDTIGQDLVNHCVNDIAVVGAEPLF
                     FLDYLGTGRLEPRVFRDIIKGFAKACAENRCALIGGETAQMPGFYAPGEYDVSGTIVG
                     VVEKSRMLDGRTIRPGDAVIGLASSGLHTNGYSLARKIFFERLRFKPRTYVPELRNTI
                     GDELLKVHMSYGPLIQKLLKTSAGRQGIRGLAHITGGGFVDNIPRVLPKGCEVIITKG
                     TWEMPPLFQLIQAKGGVAEAELYQVFNMGIGMVLLVAADQADRTLRAVHAQNHRAWLI
                     GQVAKGRGQVRVV"
     gene            complement(16164..17090)
                     /locus_tag="PKI07_07615"
     CDS             complement(16164..17090)
                     /locus_tag="PKI07_07615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOC50959.1"
                     /translation="MKTGLRLALALGCGLAVIPMTVAADNQPAPANATTDAPKLSPKE
                     QIEQWSYAIGMNIGNGIKRGGVDLDVDVLANAIKDVLAGRDLKLTEQQAQDSWRAYQM
                     ESRHKQEEMRKQQAEKNKREGEAFLAENKKKPGVKTHTVTLANGTTAEMQYKVITEGT
                     GAIPRSNDTVTVNYKGTLINGKEFDSSAKHGGQPAKFQVNRVVRGWTEALQMMKSGSK
                     WELYLPSTLAYEDRGAGPNIEPGSTLIFEMELLGTESPAPPPPPQPLTSDIIRVPSAE
                     ELKKGAKIEVIKPEDMEKYTNAPAAKGQPKKE"
     gene            17355..18650
                     /locus_tag="PKI07_07620"
     CDS             17355..18650
                     /locus_tag="PKI07_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50960.1"
                     /translation="MAAIQELKQRRVKRPSWCGPLAAAGALWLAGCVVSAPAQEAVRM
                     SMASAQAAEGRRRAATTEGYYNVKLGPMAWNFGAGLGLEYSDNVGYTQYRGEADFSLR
                     PQINARMRWPVSEWNSINLSLGAGYVAYLEQTQLNRFFISADSDSGLSLDIYAGDFYL
                     ELHDRISITEDPYRDPTVAGDGRYSRLQNAAGITTIWDLNKVILQLNYDHVNSVTLSG
                     RQGYGDLQSEVVSFSAGYAPKAGIRYGVEVGGSWQQYEGGNTYADNAWQWNVGGFGEA
                     QISEYISFAGHAGYTVYTPQTHGALTREREYSTPYVALSLRHRVNAYVDYSLSGGRTL
                     PTSLTGGAVDMYTANWSANWRVIRKTSLSTGFSYYHGSSVSSGGETFDQYGPQITLGW
                     RLARKLSSNLSYRLYWRTSDQAGRDYSVNVVSLNFNYAF"
     gene            18725..19519
                     /locus_tag="PKI07_07625"
     CDS             18725..19519
                     /locus_tag="PKI07_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOC50961.1"
                     /translation="MKSYIARACITFLAVGSLAAGALTSSVAKASPPRAPEVRDLQGL
                     QQNFPASESVPAMIIAQTSATANSGGSVVSNAASATLPILAGYVPDDKYKLRVGDKVS
                     FQIIEDRDAPRSLVVADSGELDVPYLGRVLVADKTCKQLADELKAQLEKEYYYRATVI
                     IALDVANKLLGRIYVWGQVRSQGPMDIGVNENLTVGKAILRAGGFADFANKKKVKLVR
                     AAGVDGAPRLTMELNMVDILEKGAAEKDIILQPDDFIIVPSRLISF"
     gene            19536..21734
                     /locus_tag="PKI07_07630"
     CDS             19536..21734
                     /locus_tag="PKI07_07630"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOC50962.1"
                     /translation="MNDQPVQSFHDRPNTAARLHARLSRFRNLLREKWWVLVLGAMAG
                     AIIKIVLLWNAPPSYVSVGRMIVSMKLAIQPASLYSEELSNFLGTQAALMQCGVVVNR
                     AHERVSAQKPEIGRHAVALNVLVLPKTTIFVLQATGRDRDYTQAFLQACMDEYIALKK
                     EMRSQTSDTTVAGLTEQVMRLEKDLRRAEAEMVQFQSTNSMMLTQEQGNSPAHYLTTL
                     YQRLVTLKSEYALLQRLTLDQNLARRQAAGGTFSLGNEPLAEGNDSLPDTNAVALTAG
                     GTPGQSSTAAGDRGDTAYLQAQQQLLLLRVEQQELGRYLRPNHPKMIAMQEDIARREE
                     LLKILRTQSEEQLKNRKELLAVEIEILEKDLKAWDARNQEFQHKNAEYQALKANVQRI
                     RALYDQLLATMQTLDVNKEISPESVETIEAASPAYAARLDLWKGAVNGALLGLALGLG
                     LLLVLDRLDDRVGSFTELQDLFDENVLGQVPREKLVASKRQPSLILPEDTRHSFVEAY
                     RNLRSSLLYMTEAGNRPKTILVTSSVPNDGKSITAANLAITMANAGSRVLLVDADLRK
                     GTLHRLFGVSPEPGLSDVLAKGTAWETTVSATKIANLHILPAGTFTARSSELFISEAT
                     QRFLRDAASRYDYVIFDTVPVMAADDVTCLAPQIDGVIFVLRAAFTSARVARASLESL
                     YQRQVRLLGLVFNAVRPSSEDYYYYRYKDYYRPYPSGGTKTAESARTGQA"
     gene            21896..23293
                     /locus_tag="PKI07_07635"
     CDS             21896..23293
                     /locus_tag="PKI07_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOC50963.1"
                     /translation="MVLLAGAEAEAAAKPAGAKSALTSRTAFDLGVEAYVFGYPLVAM
                     DLTRRLMCNVQEPAGLRAPMGQFARVRAFPPASNRDVTFPNADTLYAGVWLDVAREPW
                     VLELPLAKERYTQFAMLDGWNTVFGVCGARTTGDEGQKYLLTGPEWKGKLPAGMKAYR
                     SPTSIVWVLGRIHCTGTPEDYRTVYALQDQCRAVPLSSHGQPYTPPLGQIDPRLDVLK
                     TVNEQISLLSVGAYFNQLALLMKENPPTRGDAPMIKKMARLGIVPGQAFDLGSLGPDV
                     AQALQHVPQSALARIMGWFKEGARSGDWVSQDGWTWTLKTGAYGTDYTQRALVAATGL
                     GANGPEDMVQATSTVDGAGQPYSGNFRYTMHFAPGQAPSANGFWSLTLYDSEGFLVSN
                     PLGRHTLSSRDSFSFNPDNSLDFYIQKHPPGADREANWLPAPEGRFMLMLRFYWPKES
                     LLKRAWKIPPVKRAD"
     gene            23353..24676
                     /locus_tag="PKI07_07640"
                     /pseudo
     CDS             23353..24676
                     /locus_tag="PKI07_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158526.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
BASE COUNT         4317 a         7109 c         8457 g         4793 t
ORIGIN      
        1 ctgacaacgg catccggcct tgacccgcaa aagcgggcca aggcagccag ccaacgccgg
       61 ccgtagggat tccggaaggg gacgatccag gtcgcctatc cacttcctcc aatccggctt
      121 ccgggtgggc ccggttgccc gttatagcgg tttcccggtt aacggcttag cgcagcaggg
      181 tctgcccata ggtttcccgg cggcgatcct caccgatccg ctatacggac cccgtttagc
      241 acccaattcc gcctttgcca aaatcgctca aggcgctcat tttaccgtcc caaagagccg
      301 tgtacaaggg gtgtacaacg gatgctcacc ggatgtacaa ggatttttca gctgttcatc
      361 cggtgagcat ccgttgtaca ccccttgtac acggcgtatt gatagccacc ctgaagcctg
      421 taagtggctt aaaatacgga ggtttttgtg aggacgggac ttgatgatgg aggtgcgagg
      481 gctgaagcag ggttttatcg caaagtgtcc aataattgga cagtttgggg aggtgtcttg
      541 gagcgcgggt cgggggcgtc tggttgagcg ttaaggggag gataagcgtt cttgtggagc
      601 tttgctcccc ggtgccttga aatggaggag ggcacggggg ttcgctcttt ccgtttgggg
      661 gtgccggacg atttgtggct ttccaagtag cgggaggcga ttaccttgtt ggccgcagtg
      721 aatgccaacg ctcaggcaaa aacgttcggc ccggcagact tcctcaaccg ggagttaagc
      781 tggctggagt tcaatcatcg cgtgctggag gaggcgctgg acgcgcagaa cccgctgctg
      841 gagcgggtga agttcttttg cattgtcagc tcgaacctgg atgagttctt cgaggtgcgg
      901 gtggcaagcc tcaagcagca ggtcgagagc gtggtggtgg agcgttcggt ggacgggttg
      961 acggcgagcg aaacgttccg ggcgatcgtc aaacgggtgc ggcggatggt ggcggagcaa
     1021 tatgcctgct ggcgcgagga attggccccg gcactgacgg ccagcggcat ccgcctgctg
     1081 gagctggcgg agctggagcc ggctgaccgg gcgtgggtgc gggagtatta ccggacgcaa
     1141 gcgcatccgg tgctgacccc gctggcagtt gacctgaccc atccatttcc gcaggtgctc
     1201 aacaagtcgc tcaatctgat tgtgcggctg gtgaaggcgc agaacggggc ggagcgcgag
     1261 cacatggccg tggtgcagat tccggccatc ctgccgcgcg tggtgcggct gccgcgcgcg
     1321 gacgggcgcc aggattatgt ttacctggat cggctgatcg ggcattacct ggcggacttg
     1381 tttccgggca cccggctgct cggttactgg cctttccgcg tcacgcgcaa cagcgagctg
     1441 tatattgacc aggaggagac ggccaacctg ctccgggccg tcgagaacga gctgcataag
     1501 cggcgcaagg aggacgccgt gcggctggaa atagacgccc actgcccggc ggccatccgc
     1561 gaaaccctgc tgcggacgtt gcgcctgacc gaggaggatc tttacctggt ggatggcccg
     1621 ttgaacccga cgcgcctgat ggcgctggcc gaaggggatc attcccccga gttgcgcgac
     1681 ccgccgttcg tggcgccggt ggcggcgggg ttgcgccggc ggaccgattt gttcgcggcc
     1741 attcgggagc gggacctcct ggtgcatcac ccctacgaca gctttgacag cgtcgtggag
     1801 tttttgcagc aggcggcggc ggaccccgat gtgctggcca tcaagcagac gctctaccgc
     1861 acgggcggcg acccgcgcat catcggcgcg ctggagagcg ccgttcgcca taataagcag
     1921 gtgacggcgg tggtggaatt gcgcgcccgc ttcgacgagg ccaacaacat ccactgggcg
     1981 cggcagcttg acgaggccgg ggtgcatgtg gtgtacgggc tggtgggcta taagattcac
     2041 gccaagatca gcctggtcgt gcggcgcgag gggctcaata tccgccgcta cgtgcacctg
     2101 gccacgggca attacaatcc gaccacggcg aagatttata ccgacctggg cctgttcacc
     2161 tgccgcccgg actttggcga ggacgccacc aatgttttca atttgctgac gggcatctgc
     2221 cagttccagg cgcagcgcaa gctggtggtg gcgccgttcg atttgcatga gcgtctgctg
     2281 gcgctggtcg agcgggaggc gcgtcacgcc cgccagggtc tgccggcgcg catcatcctg
     2341 aagatgaact cgctggcgga gccgcaggcg atcgcggcct tgtaccgggc gtcgcaggcg
     2401 ggggtgaaga ttgacctgat tgtgcgcggc atttgctgcc tgcgccccgg ggtgccgggg
     2461 ctcagcgaga acatcaccgt gcggagcatt gtggaccggt tcctggagca cagccgcatc
     2521 ttttattttg agaacgcctg ccagccgcag gtgttcatca gcagcgccga ctggctgccg
     2581 cgcaacttct tccggcgcat cgaactggcc attcccgtgg aggacggcgt gctgcgcgag
     2641 cggctcatcc acgaggtgct ggcggtctcc cttgccgata cggtcaaagc ccgcgcattg
     2701 cgcgccgatg gttcctatca ccgcgtgcgg atggcttcgg gcgcggcggc cgtccgcagc
     2761 caggcccggt ttatcgcgct ggccgccggg gaggatccgg cggcccgtcc gtcggcgaaa
     2821 ggcaagcctc gttatccccg cgtgacgctg gccccgccgc cctttgcgcc gcggcggcaa
     2881 aagaaatgaa cccggcgcgc gcgcagcagt cccggcgggc atgggcggcc cgcccggcct
     2941 ttggccgatg aatctccggt cccgccagtc cacgcgcggc gcccggctca tggtggggct
     3001 gatgtcgggg acatcggcgg atggcattga cgcggtggtt gcggagcttt ccggaggtgg
     3061 gcggcgcctg cgggcgcggg tgcgggcgca tgtgcaccag cccttccggc cggccttgca
     3121 gcggcgcatt ctcgcagcct gccagcaggg aacggtggcg gaactctgcg agctgaactt
     3181 tctgctgggc gagcatttcg cccgcgccgc gctgcgggcg gttcggcggg cgcaactccg
     3241 gccggcggac atcgcggcca tcggctcgca tgggcagacg gtgcatcatt tgcccaacgc
     3301 acggacgcct tcgaccttgc agattggcga ggcggcggtc atcgcggagc gcaccgggat
     3361 tgccacgatt gcggatttcc gggtgcgtga tatggcggcg ggcgggcaag gcgcgccgct
     3421 ggtgccgtat gcggactggg cgctgttcac cgacgagcgg cgcccgcgca tcgtgcagaa
     3481 cctcggcggc atcggcaacc tgactttcct gccgccgcgc gcgcggctgg gcgatgtgat
     3541 ggcgttcgat accgggccgg gcaatatggt gatggatggt ctgatgaccg agttgacccg
     3601 ggggcggcag acgtttgacc gcgacgggcg ctgggcggcg cgcgggcagg tctcggagga
     3661 attgctggcg gagttgatgg cgcatccttt cctgcagcgg cgtccgccca agaccacggg
     3721 ccgcgaggag ttcggcgagg cgtttttgcg gcggctgctc ggggcggcgc gccagcggcg
     3781 cttgcgcccg gaggatttgc tcgcgacggc caccgccttc acggcggcca gcgtggcggc
     3841 ggcctaccgg cgtttcgttt tcccgcggct gacggatcgc gagcggggcc ggttgcagat
     3901 cattctgggc ggcggcggag cccggaaccc gactttgcgg agcatgctgg cccagcgcat
     3961 tggcgcgggc gaattgctga cgcacgagga tttcggcgtc gccaacgccg ccaaggaagc
     4021 gctggcattt gcgattctgg cgtacgaaac atggcgcggg aaaccgggca atgtgccgcc
     4081 ggcgaccggg gcgcggcggg cggtggtgct gggaaaaatc gtgccgggcg cggcttagcg
     4141 gcgggcccgg gggagttggg acaatcgcga tccggaaggg cgggcgtccg cgcgagccgc
     4201 ggcttttttg ccttctcccg gggctttgcg ctcgcggaga agcgcgcctg ccccggcggg
     4261 cggaggcggg gccgtattaa agcggcttga ttccgccgcc gcgtcgggcg taatgagaag
     4321 cttatggcca tcctgaatat caaaccggaa ggttcgggca aatacgatct gatcgcgttg
     4381 ggtgaagtca tgctgcggct ggacccgggc gaagggcgcg tgcgcacggc gcggcagttc
     4441 aaagtgtggg aaggcggcgg cgagtataac gtcgcgcgcg ggctgcggcg ctgctttggc
     4501 ctccggaccg ccatctgcac cgcctttgcc gataatgacg tggggcggtt gctggaggat
     4561 tttatcctgc agggcggggt gggcaccgag ttcatcaagt gggtgccgtt tgacggcgtg
     4621 gggcgcgccg tgcgtaacgg gctgaacttc accgagcgcg gtttcggcgt gcggggcgcg
     4681 gtggggattt ccgaccgcgg ccacagcgcc gccagccagc tccggccggg cgactttgac
     4741 tgggacggcc tttttgggcg gctcggcgtg cgctggctgc ataccggggg cattttcgca
     4801 gccctgagcg ataccacggc ggagttggtt ttagagggag tgcaggccgc ccggcggcac
     4861 ggagtcattg tttcctatga cctgaactat cggccctcgc tctggaagag cagcggcgga
     4921 cttcaacgcg cgcgggaggt caatcggagg attgcccgga atgtggatgt gatgctgggc
     4981 aacgaggaag acttcaccgc gtgcctgggg ttcgaggttg ccggcgcgga cgaacacctt
     5041 tgccggctgg aggcggcggc cttccagcag atgatcgagg aggcggtgaa ggcgtatccg
     5101 aacttccgcg tggtggccac cacgctgcgg gcggtgaaga ctgccacgcg gaatgactgg
     5161 ggggcgattt gctgggcgga tgggcggttc taccaggccc cgaaccggga ggatttagag
     5221 attctggacc gcgtgggcgg tggcgatagt tttgccagcg gcttgattta tggcttgctc
     5281 aagtttaacg acccccagcg ggccgttgcg tatggcgcgg cgcacggcgc cctggccatg
     5341 accacgccgg gagatacttc gatggcctcg ctgccggaag tggaggcgct tgtcaaaggc
     5401 ggcagcgcgc gcgtgcagcg gtgacgaaag cggattggcg cgccgccccg gggtggtttc
     5461 agcgccgcgc ctggatcagc agcagccagt cgcccggggc tggcgtggtg aaccgcccgc
     5521 cccgggcgac tgcggcctgg cggctgccgg tgcgggggtt gaaccaactc gccgccggcg
     5581 aggcgggcag ggcgtcgggg cggagttgga cggagcggtc ttccggcaca tagaccacgg
     5641 tcaaggtgtt ggcctccgtg ctggcggcgg cgataaatcg ttgcggcgag cggcgcccgg
     5701 gttgcgcggc gacccggtgg gtttgcgggc ggagcgcttc gtagttgagc gagttcatga
     5761 atgttgccag gtgcgacatt tgctttgcgc cggacagggt tagcgcctca cgccacagcg
     5821 tcccgccgcc tcgcttgggt ttcccgtcaa ttccagccca ggggatcaat tcctcggcgc
     5881 cgtagctgac gccggccggc ggggccagga gcaaacccca atacgcggcg cgccgcacat
     5941 cgtcggcggt gaatcgccgg gccgaccgtg gggcgatgct gctctcatgc ggcgcgaaca
     6001 caatcaatgg ccgggccggg gctcgctgcc actccgccgt gaacggcccg gacactgccc
     6061 acatcagggc gtcgtccgtg acatccgtga cgttttgata gccgaaggca tccacccact
     6121 cctggtcacg gaattcgtcg aacagccaat gcgtccggcc gggaaatagc accaccggcg
     6181 cgtgcgggcg gtcgccaaac accgtctgtc caatgcgttt ccagcgctca aggccatggg
     6241 tggctgcgtc gcttttgatg gccagcagcc aaaccaccgg ctcggcgctc cagcgcgcga
     6301 ccatatagcg cacgaggcat tcggcctggt cgtcaggcaa tggaacggcg ttggttggcc
     6361 atggctgctc ccatagcggg acgattgcgc ttaccaggcc cgcctgggcc agggcggtga
     6421 ttttcgcgtc gagccgctgg aaaaaggcgg ggttgatggt gatacgctcg ggggaactgc
     6481 cccaggcgga ctgggctcgg gcatcctcgc ccggagccgc ggcccattgg acgaccgtga
     6541 aatcctggcg cgcgcgggtg ctggcgtagt aatcccagtc ccgggattcg gctaagcggg
     6601 cgccgtccca aacggtgtcc gccagccaga agaagggggt gccgtcggtg tgttcgaaat
     6661 ggcggcgatc gcgcgcgacg cgcacgggtc cgtgccgatg ccacggggtt gcgccggtgg
     6721 ccgccgtgca gaggaattcg ccggcctggt cgtgcaggcc gggattggtg gggtcggagc
     6781 aagccgtgcg gaagcgccag gggccgggtt gatccggcgc aaatcgcacc cgccaggtct
     6841 tgcctccatc ccaaaaggcg tccacttcgg ccgtttcgcc cagcggcgag gtgaatgtga
     6901 ctttcaggga gacgtcctgc accgggtggg cataggcgac gctgctcttg aaagcccgtt
     6961 cgcaacgatt ccatttggga accagttcca tgcggtcggc agccggcgcg gcaggaagcg
     7021 ccgccgccag catccacgcg aaaacaaccc gccgcggccc gttcgagatc cagttgcgca
     7081 tcatgtgaat tccctttttc tcgttctgcc atcatcagac agcaatcgca ttgtcctgtc
     7141 aaaccccggg tggcgccggg ccgggggaaa gtgtctcgcg cgaaatgatt ctattggcga
     7201 aagcgccgga ttggcttatg gttctgcatt cctgaaccta aacacactta tggaacctag
     7261 agctgacatc gctgtgatcg ggctggctgt gatgggccag aatctgattt tgaatatgaa
     7321 cgaccgcggg tttaccgtgg ccgcctacaa ccgcactgtt tcgaaagtgg acgacttcct
     7381 caatcaggaa gcccggggca ccaaagtcat cggcgttcgc tccatcgcgg aaatggtctc
     7441 ggtgctgaag aaaccccgcc gcgtcatgct catggtcaag gccggcaaag cggtggacga
     7501 gttcattgac caggtcatcc cgcatctcga agcgggggac atcattattg acggcggcaa
     7561 ttcgctgttc caggacacga tccgccgcac ccgctacgtc gagtcccggg gcctgctcta
     7621 tattggcact ggcgtttccg ggggcgagga gggcgcgcgc cgcggcccga gcatcatgcc
     7681 cggcggctca ccggccgcct ggccgcacgt gaaggacatc ttccagcaaa tcgccgccag
     7741 ggtggacgac ggcacgccct gttgcgactg ggtgggcgaa aacggcgccg gccattacgt
     7801 caagatggtg cataacggca tcgagtacgg cgatatgcag ctgatttgcg aagcctacca
     7861 tctcatgaag gccggtctcg gcctgggcgc cgatgaatta caccgggtat tcgccgaatg
     7921 gtaccagggc gaactgaact cttatttgat agagatcacg cgcgacatct tcggcgtcaa
     7981 ggacgccgat ggccagccgc tggtggacaa gatactcgat acggccggcc agaagggcac
     8041 cggcaaatgg acggtcatct cctcgcagga cctgggaatc cccattacgc tcatcgccga
     8101 ggccgtctat gcgcgctgca tttcggcgct caaggatgac cgcgtggcgg cggcgcggaa
     8161 gctcaagggg ccgcggcccg cgatcaaggg tgacgcgaag cggctggtgg aagacatccg
     8221 gcgggcgttg tatgcctcga agatcatttc ctacgcgcaa ggctacctgc tcatgcgcgc
     8281 cgccgccagg gaatacaact ggaatctcaa ctacggcggc atcgccctga tgtggcgcgg
     8341 cgggtgcatt atccgcagcg ttttcctggg caagatcaag gaagcgttcg accgcaagcc
     8401 agacctcacg aacttgctgc tcgacccgtt cttccggcag gcgatcaagg attgccagcg
     8461 gtcatggcgc aaggtggtgt ccctggccgc ccgcaaaggc attccggtgc ccgccttcag
     8521 caccgcgctg gcgttctacg attcctaccg cagcgcctgg ctgccggcca acctgctcca
     8581 ggcccagcgc gattacttcg gcgcgcacac ctacgagcgt gtggaccagc cgcgcgggca
     8641 gttcttccat accaactgga cgggccgcgg cggcaccacc gcctcgagca cctacacggt
     8701 ctagcgccgc gcggcgggca ctaatcctct ttgtccgccg ccgcgtccag ccgccggaac
     8761 ttgatctcgc gcagggcggt ggtggtctgc caggccgcca gcccgagcgg ttgcgaaagc
     8821 tcgatgtcgc ccggccggac cgagaacttt cggcccgccg tggccaaatc caccagcttt
     8881 tcctcgtcaa tccaggccgt gatccgttgc tccgtcaccc gaagccggat gcggtaccag
     8941 cgtccggact cgaagttccg gaacttggtc gtctcgttct ctgcggcgtc caggccatcc
     9001 acacacgaaa gccccaccag cgagccgccc cagcctccca cgatcaggct gcagcagttc
     9061 gtcccgaccg gcacggtcag cccgcagaag aaatccgaac ccgtcacgcg catggcgtcg
     9121 agcgcgatct cgtagttccg ttggggaaag tcgttcgtcc aattaatccc ggtaaacggg
     9181 ttgcccatgt gcaggatgat tgccccgggc tggctctgta tttcgccgcg gccggtaaac
     9241 ggcgtttcgc gccagcccgc cagcgtccgc ccgtcggaca tcccctgcca accccgcccg
     9301 gcaaaaggcg cgggcggccg ggctgcgccc ggcggcagtg tcagcggggg ggcttcgtcc
     9361 tccgcaggag gcggaaccgc cggggagggt ggcggcgccg gatcgggcgg gagcggggtt
     9421 gtttcggagg cgcatcccgc cgccaggatt aaggcggctg aaagcgccaa gggccacatc
     9481 catttaatgc gcgtgttgct catgctgctt tgtcgggctt acactagctt ttcgcgcggt
     9541 tttgagccag aattgatttg cgccttatga acttgccagc ttttctacgc gcccaaccgg
     9601 acggcgtgct tctttcggtc aaagtccagc cgcgcgcctc ggccaatgaa atcggcgaag
     9661 cgctcggcaa cgaactgcgc gtcaaagtca ccgccccgcc cgtcgaggcc gccgccaatg
     9721 aagccctgtt gaaattactg gcgcagcaac tgcgctgccc tcgcaaccgg gtggacatcg
     9781 tccgcggcca tacctcccgc cataaaaccg tgaagcttta cggcctgagt ccggaaaaag
     9841 tccttgccca gttaggccag gcctgaaccc cgcccggaat gccgggcact gcccgccccg
     9901 cgagccggcc cttcaacccc agcctctctc cggacaccgc ccctccggcc agcatcgtca
     9961 ttccgggcta cccccgtcat ttatccggtc tcccgcgccg tgtacaaggg gtgtacatcg
    10021 gatgcaccac ggatgaacaa gggattttcc gttgtatatc cgctgagcat ccctggtaca
    10081 ccccttgtac accccttgta cacggcttac ggatagctat ggaagagcct gaaatgagct
    10141 gagttgcgta aaattcggct tgagggcggc tgaaacggtc aatggggtgc ccgaaagggt
    10201 gttttgggag ggggccaata cttgggcgtc tttggcggga gaggcgagga aagggttggc
    10261 gggggattgc agcttgcaag ttcattgggg ccgattatgc tggtggccgc agacgcagcc
    10321 taaacttgga aatgagcacg aagtcgggca aaacgaagaa agtcaccgtt ctacttgccg
    10381 atgaccatat tgtggtccgg caaggattgc gtgctttgct ggaagctgag ggtgacatcg
    10441 aggtggtggg ggaggccaag gatggccgcg aggcggtatc tttggctcgg cagacccaac
    10501 cggaggtcgt ccttatggat gtggccatgc cggagttgaa tgggcttgag gcgacccggc
    10561 agattgtcag ggagcttccg gggaccaagg tgctggcgtt gagctcgtat gggaacgatg
    10621 attacgtgac gcaattgctg gaagcgggcg gggttggtta cctggtgaag caaacggcgg
    10681 cggcggactt gctgaaggcc atccgccagg tgcggaaggg gcaggtgttt ttcagcccga
    10741 tgattgccca gcgcctgcag gcgggaaggc cggggacgcc cggcggcggg cggccgggca
    10801 ataagcatgc cagcctgtct tcgcgcgaag cggaagtgct gcagttggtc gcggaggggt
    10861 ttgccaacaa gcaaatagcg gccaaactct ccatcagcat caagacggtg gaaaagcatc
    10921 ggcagcaggc gatgaacaaa ctcaacattc acgacacggc cggcctgacc cgctatgcca
    10981 tttcgcgcgg ttgggtggaa tcgggtgttt aaccggaaat ccgcgaaagg agcaccccat
    11041 gttttctgaa caaatcgagc tgcgcggcca cattatagat tccctgctgc tgccgaaagt
    11101 gctggacgag atcctcacgc gcggcggcaa cttcaccatc cgggagattc aggtcggcaa
    11161 gcagcgggac gacgcgagtt atgcgcgcat cgaggtttac gggccctcgc ccgaagtggt
    11221 ggatgatata gtgcggcacg tgcgccagca gggcgcggag gtggtcgagc aggaatctgt
    11281 gaggctggtg gaggcgcccg cggacggggt gtttcccccg gatttctacg tgacgaccaa
    11341 ccagaagacg ttcgtgcatt atcgcggcca atggctcgag gtgcggcccg tgacgatgga
    11401 ttgcgcgatt gccgtggatg aacagcgttt gcaggcggag gcggtgaaat tcgcgcaggt
    11461 gcaaaaaggc aggttgatcg ccgtggggca tcatgggatc cgggttgccc cccgggagcg
    11521 gccgggcgag cggcggagcg tgttcgagtt catgggcagc agcgtgtcga gtgagaagcc
    11581 caagagcgcg attgtccgcg aggtggcggc ggaaatgcgg cgggcccggg aatcgggggg
    11641 gcggatcctg gtggtggccg ggccggcgat tgtgcatacg ggcgccgggg agcatttcga
    11701 gaagttgatt gcgcaaggct acgtgaaccg gctgtttgcc ggaaatgccc tggcggtgca
    11761 tgacatcgag aacgcgctgt tcgggacctc cctgggcgtt tacctggagc gcggctcgct
    11821 ggcggagaag gggcatgaga atcatatgcg ggccatcaac gcgattcgcg gggcgggggg
    11881 cattgccgcg gcggtcgcgg ggggaatcct ccggcggggc atcttttatc aatgcgtaaa
    11941 gcataacgtg gattttgtcc tggcgggctc gattcgcgat gatgggccgc tgccggaagt
    12001 gattacggat acgctggccg cccaaaagct gatgcgcgcc cggctggaag gggtcacgat
    12061 ggtgctgatg atgtgcacca tgctgcactc gatcgccgtt ggcaacctgt tgccggcgca
    12121 cgtcaaaacc atttgcgtgg acatcaatcc ggcggtggtc accaaactga gtgaccgggg
    12181 cacgtttcag gccgtagggc tcgtgacgga tatcgagccg ttcctgcgcg aattaaccga
    12241 atttctggcg gctggcgaaa gcccggctgg cccggtttaa ggggaagact ccgggaacag
    12301 cggcgagcgg gggatggtaa tgggcgtccc tggcttgagc cggtggaagt ccacccgggg
    12361 attggccgcg cggatggcgc tctccggcag cccgtacatc agggcaatgg tcgccgcgct
    12421 gtctcccgga ccgaggacgt acacccgccg gggctgcggc gcgggcggca gcgggggcag
    12481 gtcgggcggg acgagttgat cctccagcgc ccgttgctga gttgcggtca ggcggttggt
    12541 ttccgcggcg tggtaggccg ccaggtagga ggcgtagagc gcacgggcga cctccgggtc
    12601 gccgtcagcg tcttcgagtt tttcctgata aagcgggtcg tggagcagcc ggtattcctc
    12661 gtaccgtttg gggccgagcg cgattttgat ggcgttttcg cgctgatctt ccagcgcctt
    12721 tcgcgtcgcg aggctggcgg gatcccgggc atcggccagc aagtggagtt gctgatccaa
    12781 agcgtcggtg gcgcggaaaa cggcgcggaa ctcgtcgggt gagggatcga agaggcggag
    12841 ctggccgaat tgcgcgcgga ggtcattggc gttctgggaa tagcgcagca gatattcttc
    12901 gagctcgggc ggagcgagca ggtgggcgag gtcggcgcgg gtttgctggc ggagcttggc
    12961 cagttcgcgc gggtcggggc tcaggccctg gcggctttgg gcgtcgaggt aggcctgcaa
    13021 gcgctcttgc gagcgggcgt tgatttcctg gatgttcttt ttggtttcgt cgggcagatt
    13081 ccccagcacg gggccatcca gcgtgacgct caggcgggag gggcggggga ggctgaccag
    13141 gtcgccggtt tcccaactgt tgcccaggag gctggtcagc agggcgcgtc gttcttcctc
    13201 cagttcctgg gccttttggg cggcggcgcg gaggacgttg agatccggct cgctgcgcca
    13261 ccattgctgc tcgccggtca ccagttcggt ggcccgcttg cgcgcataaa ccgcgttcac
    13321 gtccgcgatg atgatatcgc ggatggtttg ctcggggcag ccgatatcgc gcaggttcgc
    13381 aatataattc gtgtagttgg gggactcgat gtcccgccag tagaagagtt ggcggcgcag
    13441 aatgatgttg gtgcgcgccg gcgggggcgg aggctgctcc gcggccagcg ccgaggcggt
    13501 ggcgcgttga gccgcgtttt cccggatgac agaccaccac gccgccgcca ataggacatt
    13561 gacgccgagc gacacccacg caattactcg ccagcgcata ctggtattta tcggcaaatc
    13621 acggcggctg taaattgaaa accgccgcag gagaaaagtt ttgggccggc gcgattggct
    13681 tttgccaggg ggaaaggttt ttggtatgtc tgcgggcgat tgatccattg cccgcgatac
    13741 ccgctatgac cacgaccgct tcgacccggg aagccggcgg cgccacgcgc gcccaatggc
    13801 tggtgctggc cgcggccttt ttgggctgga tgtttgacgg ggtggagatg ggaatctttc
    13861 cgttggtggc gcggccggcg ctccaaagca tgctcccggt ttccacgccg ggacaggatg
    13921 ccctggtggg gctgtggatg gggcgtatca ccgccttgtt tctggtgggg gcggcattcg
    13981 gggggctggg gtttggctgg ctgggagacc gcgtggggcg ggtccgggcc atggtgctga
    14041 gcattctgac gtattcaatt tttaccggcc tttgcttttt tgcgcgggcg ccctgggagt
    14101 tgggcgcctt gcgttttatc gccgcatttg gcatgggcgg ggaatggtcg ctgggtgtgg
    14161 cgctggtgat ggaggcctgg ccggcgccga ggcgcgcgtt gctggcgggg attatcggca
    14221 cggcgtccaa cgcgggatat ttgctcatcg cgctggtcgg gctttgcttt caggtcaccc
    14281 gggagtcatg gcgctggatt atgatcgccg gggcggcgcc cgcgtttttg gcgctgggga
    14341 ttcagtgggt ggtgccggaa tcggaacgtt ggaaggcggc gatgcagcaa ggggcggctc
    14401 gtccgctccg ggagatcctg tccgcgggcc tgctgggcaa gaccttgctg ggggtcgccc
    14461 tggcggcggt ggcgctgatc gggacctggg gatcggtgca gtggctgccg ctctgggcgg
    14521 atcaaatgac cggcgggcag aaccccgccg ccaaggccgt ggcgcaaatc ctggtcagcg
    14581 cgggggcaat cctgggatcg ctggccgggg cgctggtggc ggggcgcctc gggcggcggc
    14641 cggcctattt tgttttgagc ttgctgtcgt tgctgctctg cggcgggctt ttccgcgggg
    14701 tgaatcacta cggcgcggtg ttttgggtga tggtgacgct ggtaggggcg gcgacggcgg
    14761 cgttttacgg ctggctgccg ctctatcttc cggagttgtt tcccactcgc gcgcgcgcga
    14821 ccggccaggg cgtttgctat aacgccgggc ggcttctggc ggcggcgggc gcgttgacgc
    14881 aggggcaatt ggtcgccgcc tatggcggca gctacgcccg tgcgggagcg acaattacgc
    14941 tcatttatct gctgggcctg gggcttattt ggctggggcc ggagacccgg gaccagccgt
    15001 tgccggagta ggcccgggat gctggcgcag cgtggcgggc gcaaggccgg ttttcccgag
    15061 agatcgctta agcttggaca gcggcccgcg acgcgctacc ttgccgcgca tgaaatcaaa
    15121 ggcatacgca cgcgcgggtg tggatataga cctgggcaac cgggtcaagg ccgatctgcc
    15181 gcggatgctc caggccacgc accgtcccgg agtgctgggc aagatcggcg ggttcggcgg
    15241 cctgtttgag ttggaggtgc gcaagtatcg ccagccggtg cttgtctcca gcgtggacgg
    15301 cgtcggcacc aagctcaaac tggcgtttgc gctggaccgc catgatacga ttggccagga
    15361 cctggttaat cattgtgtga acgacatcgc ggtggtgggg gcggagccgc tcttctttct
    15421 ggactacctg ggcaccggcc ggctggagcc gcgggtgttc cgggatatta tcaagggctt
    15481 cgccaaggcc tgcgcggaga accgctgcgc gctcatcggc ggggagacgg cgcaaatgcc
    15541 ggggttttat gcgccggggg aatacgatgt aagcggcacg attgtggggg tggtggagaa
    15601 gtccaggatg cttgatggcc ggacgattcg gccgggtgat gccgtgatcg gcctggcttc
    15661 cagcggtttg cataccaatg gttattcgct ggcccgcaag atcttcttcg agcggctgcg
    15721 cttcaagccc aggacctacg tgcccgaatt gcgcaatacc atcggcgacg aattgctcaa
    15781 ggtgcacatg agctacgggc cgctgattca gaaactattg aagacatctg ccgggcggca
    15841 gggaatccgg ggcctggccc acatcaccgg ggggggattc gtggacaata taccccgagt
    15901 gctgcccaaa ggctgcgagg ttatcatcac gaagggaacg tgggagatgc cgcccctctt
    15961 tcagctcatt caagcgaagg gcggcgtggc ggaggcggag ctgtaccagg tgttcaatat
    16021 gggcattggc atggttctcc tggtggcggc tgaccaagcc gaccggacgt tgcgggccgt
    16081 ccatgcacaa aaccatcgcg cctggcttat tggccaggtc gcgaaaggca gggggcaggt
    16141 gcgggtggtt tgacccgcga acgttactct tttttcggct gcccttttgc ggccggggca
    16201 ttggtgtatt tctccatgtc ctccggctta atgacttcga tcttggcacc cttcttgagt
    16261 tcttcggcgg aaggaacgcg aataatgtcg ctggtaagcg gttgcggagg cggcggcggg
    16321 gccgggcttt ccgtgccgag cagctccatc tcgaagatca aggttgagcc gggctcgatg
    16381 ttgggacccg cgccacgatc ttcgtatgcc agggtcgagg gcagatacag ttcccatttc
    16441 gacccggatt tcatcatctg caacgcttcg gtccagccgc gcacgacgcg gttgacctgg
    16501 aacttggccg gctggccgcc gtgtttggcg gagctgtcga actctttgcc gttaatgagc
    16561 gtgcctttgt aatttacggt aacggtgtca ttactccggg ggatggcgcc cgtgccttcg
    16621 gtgatgactt tgtattgcat ttcggcggtc gttccgttgg ccagcgtcac ggtatgcgtc
    16681 ttgacgccgg gcttcttctt gttttcagcc aggaacgctt ccccttcccg cttgttcttt
    16741 tccgcctgtt gcttccgcat ttcttcctgc ttatggcggg attccatttg ataggcgcgc
    16801 caggagtcct gcgcctgctg ttccgtcagc ttcaaatcgc ggccggccag cacatctttg
    16861 atggcatttg ccaggacatc cacgtccagg tccacgccgc cgcgtttgat tccgttgcca
    16921 atgttcatgc caatggcgta actccactgc tctatttgct ccttggggga aagtttgggc
    16981 gcgtccgtcg tcgcattcgc cggggccggc tggttatccg ccgccaccgt catgggtatt
    17041 accgccaacc cgcatcccag cgccaaggcc agtcgtagtc cagtcttcat agtcactcga
    17101 tgaatgattg tttattcagc gttgtttcgt ttacaccaat ggggccaaat gtccagcagg
    17161 aatattccgg attctgcttt acagaccggg gcgctttggc gaggctcatc ctccgccgca
    17221 aggccgggcg tccaaagccg caggcgcggg ttgatggggt tttcgtgggc agtccgtggc
    17281 gcgaactgcc gaaggccggg gcgcgggaag ctcgcattgc gaaacggaag tgaattttaa
    17341 caggttgcgc gcaagtggcc gccattcaag agttgaaaca gcgacgcgtg aagcgccctt
    17401 cgtggtgcgg gccgttggct gccgccgggg cactctggct ggcggggtgc gtcgtttccg
    17461 cgccggcgca ggaagccgtg cgcatgtcaa tggccagtgc gcaggccgcg gaggggcggc
    17521 gccgggcggc tacgaccgag gggtattaca atgtcaagct cggccccatg gcttggaatt
    17581 tcggcgccgg gttggggctg gagtatagcg acaatgttgg ctacacgcag tatcggggcg
    17641 aagcagactt cagcttgcgc ccgcaaatta acgcgcgaat gcgctggccg gtttcggagt
    17701 ggaacagcat caacctgtcg ctgggggcgg gttatgtggc ctacctcgaa caaacgcaac
    17761 taaaccggtt cttcatctcg gccgattcgg attccgggtt gtcgctggat atctatgccg
    17821 gcgatttcta tctcgaactg catgatcgta tctcgattac ggaagatccc tatcgggacc
    17881 cgactgtggc tggcgacgga cgctattccc gattgcagaa tgccgcgggg ataacgacga
    17941 tctgggattt gaacaaggtc atcctgcaac tcaactacga tcatgtaaac agcgtcacct
    18001 tgtcggggcg gcagggttac ggcgatttgc aatcggaggt tgtctctttt tccgcgggct
    18061 atgcgcccaa ggccgggatt cgctacgggg tagaggtggg cggatcctgg cagcaatacg
    18121 agggcggtaa cacctacgcc gacaatgcct ggcaatggaa cgtcgggggt tttggcgaag
    18181 cgcagatcag cgagtatatc agctttgcgg gccatgccgg ctataccgtg tacacgcccc
    18241 aaacccacgg agccttgacc cgggaacgcg aatacagcac cccctacgtg gctttgtctc
    18301 tccggcatcg cgtcaatgcc tacgtggatt attcgctgag cggggggcga acgcttccca
    18361 cctcgcttac gggcggggcg gttgatatgt acacggccaa ttggagtgcg aactggcgag
    18421 tcatccgcaa gacgagtctt tccacaggat tctcttatta tcatggttcg agtgtgtctt
    18481 ccggcggcga aacatttgat cagtatggcc cgcaaatcac gctgggctgg cgtctggcca
    18541 ggaagctgtc cagcaatctg agctatcggc tctactggcg cacctcggat caggcgggcc
    18601 gggattattc cgttaatgtg gtgagcttga acttcaacta tgcattttag cgatgctctt
    18661 gggcgctcgt gaaagaagct ggcatttatt tcggaaactg gtataaggga aaggcactaa
    18721 gaatttgaag agctacatcg caagagcctg tatcacgttt ctggccgtag ggagtctggc
    18781 tgccggggcc ctgacttcgt cggtcgccaa ggcatctccg ccccgggccc cagaggttag
    18841 agacctgcag gggctgcagc agaacttccc ggcgtccgaa tcggttcctg cgatgatcat
    18901 cgcccaaaca agcgcgacgg ccaacagcgg gggttctgtg gtaagcaacg ccgcctccgc
    18961 tactctgccc atcctggctg gttatgtccc ggacgataag tacaagcttc gggtcggaga
    19021 taaagtcagt ttccagataa ttgaggaccg cgacgcgccg aggagcttgg ttgtggcgga
    19081 ttccggtgag ttggacgtgc cttaccttgg ccgggtgctg gtggccgaca agacctgcaa
    19141 acaattggcc gatgagctga aagcgcaact ggaaaaggag tattattacc gggccaccgt
    19201 gattatcgcg ctggatgtcg cgaacaagct tttgggccgg atttatgtgt ggggccaggt
    19261 gcgctcccag gggccgatgg acattggcgt caatgagaat ttgactgtgg gcaaggctat
    19321 tttgcgcgcc ggcggtttcg cggattttgc gaacaagaaa aaggtgaaac tggtgcgggc
    19381 agcgggcgtg gacggtgctc cccggctgac gatggagtta aacatggtgg atattctgga
    19441 aaagggcgcc gccgaaaagg acatcatcct gcagccggac gatttcatca ttgttccttc
    19501 ccggttgatc agtttttgaa gccccgagcg ctcgcatgaa tgatcaacct gtccaaagtt
    19561 ttcatgaccg gcccaacacg gcggctcgcc ttcatgcccg gctgagccgt ttccggaatc
    19621 tcctgcgtga gaaatggtgg gtattggttc tgggcgcaat ggcgggggcg ataatcaaga
    19681 ttgttctgct gtggaatgcg cctccgtctt acgtctcggt cgggcggatg attgtcagca
    19741 tgaagctggc cattcagccg gcatccctct attcggagga attaagcaat ttcctgggaa
    19801 cccaggccgc cctgatgcag tgcggcgtgg tggttaaccg cgcgcatgag cgggtgagcg
    19861 cgcagaagcc cgagatcggg cggcatgcgg tagcactcaa tgttttggtg ttgcccaaga
    19921 cgacgatttt cgtcttgcag gccacgggcc gcgaccggga ctacacgcag gcattcctgc
    19981 aggcgtgcat ggacgaatac atcgcgctga agaaggaaat gcggagccag acttccgaca
    20041 cgacggtggc cggcctgacc gagcaggtga tgcggcttga gaaggatcta cgccgtgccg
    20101 aggcggagat ggtgcagttc caaagcacta acagcatgat gctgacccag gagcagggca
    20161 acagcccggc tcattacctg accacgctgt atcaacggtt ggtgacgtta aagtcagaat
    20221 acgcattgct gcaaaggttg accttggatc agaacctggc acggcggcag gcggcgggcg
    20281 gcactttttc tctggggaat gagccgctgg cggaaggaaa cgattctctg cctgatacta
    20341 atgccgttgc attaacggca ggcggaacac ctggccagag ttcaacagcc gccggagatc
    20401 ggggagacac cgcctacctc caggcgcagc agcaattatt gctgttgcga gtcgagcagc
    20461 aggagcttgg gcgttatctg cgccccaatc atcccaaaat gatcgccatg caggaggaca
    20521 tcgcccgccg ggaagaattg ctgaagattt tgcgcacgca aagcgaggaa caattgaaga
    20581 accgcaagga actgctggcc gtggagattg agatactgga gaaagatttg aaagcctggg
    20641 atgccaggaa ccaggagttt cagcataaga atgcggaata ccaggcactg aaggccaatg
    20701 tgcagagaat ccgggcgctc tacgaccagt tgctggccac catgcagacg ctggatgtca
    20761 acaaagaaat aagcccggag agcgtcgaaa ctatcgaggc ggcgtcgcca gcctatgcgg
    20821 cgcggctcga tttgtggaag ggtgcggtga acggcgccct gttggggctg gcgctgggcc
    20881 ttgggttgtt gctggtgctg gaccggctgg atgatcgcgt gggctcattc acggagctgc
    20941 aggacctgtt tgatgaaaat gttttggggc aggttccccg agaaaaactg gttgcttcca
    21001 agaggcagcc cagcctgatc ttgccagagg atacacgcca ttcattcgtc gaagcgtacc
    21061 gaaatcttcg ttcctcgctg ttgtatatga ccgaggcggg aaaccgcccc aaaaccatat
    21121 tggtaaccag ttcggtgccg aacgatggca aatctatcac cgcagcaaac ctggcaatca
    21181 ccatggccaa tgccggttcc cgcgtgctgt tggttgacgc cgatttgcgc aaaggcacgc
    21241 tgcaccggct ttttggcgtt tcgcccgagc cgggcttgtc ggatgtgctg gccaaaggca
    21301 ctgcctggga aacgacggta agcgccacga agattgctaa tttgcacatt cttcccgcgg
    21361 gcaccttcac agcccgctcg agcgaattgt ttatcagcga agcaacccaa cggttcctgc
    21421 gggacgccgc cagccggtac gactatgtga tattcgatac cgtgccggtg atggcggcgg
    21481 acgacgtaac ctgcctggcg ccgcagatag acggcgtgat tttcgtgctc cgggcagctt
    21541 ttacctcggc ccgcgtggct cgcgcctccc ttgaatcgct gtatcaacgc caggtccggt
    21601 tgctcgggct tgtattcaat gcagtccgcc caagttccga ggactactat tattatcgct
    21661 ataaggatta ttatcggccg tatccgtccg gcggaaccaa aaccgcggaa tcagcgcgga
    21721 cagggcaagc ctgacccggg cgcgctgcgc ggcatgaaac atgaacggag gttcgcccag
    21781 tgacgccatg gcccgagtcc cctttagtag accgcatcca ctcgggatga ggccggtgag
    21841 ggctaaagcc acgaggcgcg ttcttgcctg cagggcatgg gccggcctgt tgactatggt
    21901 gctgttggcg ggggctgaag ccgaagcggc agccaagccc gccggcgcca agtcggcgct
    21961 gacttcgcgg accgcgttcg acttgggagt cgaggcttat gtgttcggct acccgctggt
    22021 ggccatggat ttgacccggc ggctgatgtg caacgtgcag gaacccgcgg gcctgcgtgc
    22081 cccgatgggg cagtttgcgc gcgttcgggc tttcccgccc gcctcgaatc gcgacgtgac
    22141 ctttcccaat gccgacacct tgtatgcggg cgtttggctg gacgttgccc gcgaaccctg
    22201 ggtgctggaa cttccgctgg cgaaggagcg ctacacccag tttgccatgc tcgatgggtg
    22261 gaatacggtg tttggagtct gcggcgcgcg gacaaccggc gacgaggggc agaaatatct
    22321 ccttaccggc cccgagtgga agggcaagtt gccagccggg atgaaggcat accgctcgcc
    22381 caccagcatc gtctgggtgc tggggcgcat tcactgcacg ggcacgccgg aggactaccg
    22441 gacggtgtat gcgctgcaag accaatgccg ggccgtcccg ctcagttcgc atggccagcc
    22501 ttataccccg cccctgggcc agattgatcc ccgcctcgac gtgctgaaga ccgtgaacga
    22561 acaaatcagc ctcctgagcg tcggcgccta ctttaaccag cttgccttgc tgatgaagga
    22621 aaatccgccc acccgcggcg atgctcccat gattaagaag atggcgcgcc tgggcatcgt
    22681 gcccgggcaa gcgttcgacc tcggcagcct cggcccggat gtggcccagg ctctccaaca
    22741 cgtcccccag tcggccctgg cgcggatcat gggctggttt aaggaaggag cccggagcgg
    22801 cgattgggtc agtcaggacg gttggacgtg gacgctgaag accggcgcct atggcactga
    22861 ctacactcag cgggcgctgg tggcggcaac cggccttggc gccaacggcc ccgaggatat
    22921 ggttcaggcg acctcgaccg tggatggcgc cggccagcct tacagcggga atttccgcta
    22981 cacaatgcac tttgccccgg ggcaggcccc gagcgccaat ggcttttggt cgctgacgct
    23041 gtatgacagc gagggcttcc tggtcagcaa tccgctcggc cgccataccc tcagttcgcg
    23101 cgattcattc agctttaatc ccgacaactc gctggatttc tacatccaaa aacatcctcc
    23161 cggcgcggac cgggaagcca actggttgcc ggctccggaa ggccggttca tgctcatgct
    23221 gcggttctat tggccgaagg agtcgctgct caagcgcgcc tggaaaatcc cgccagtcaa
    23281 gcgggcggat tagcccgaaa acttcactaa aaaaagtcgc aacggaggtc tcgccccaat
    23341 atctattggg taatgatcaa cgagaaccac accgctgcga ctgggacagt ttgtaactct
    23401 gaagtcttca cttttccagc cgtaaaaaag tgtgccgtcc aggccagttt caccggcggg
    23461 gaggtgagca gcgatggcgg catcgcactc ttgcgccaga ccgaccggcg gattggcctg
    23521 acgcgtgcgc tggcccaagt gctgcccgat ccacgtgatc ccggccggat caagcatccg
    23581 ctgctttcgc tgattcgcca gcgcctctac ggattggccg ccggttacga agacctcaat
    23641 gatcacgaca ccttgcgcca cgatctggct tggcaaaccg ccgtggaacg tgaccacgca
    23701 ttggcctcca gtcccacgtt gtgccggctg gaaaaccggg cgaaccggca ggtcgcctgg
    23761 gcggtgcatg cggggttgat caaccagttt atcgcttcgt ttcctcaaac gcccgccgag
    23821 ttgattctgg atttcgacac gaccgacgac cgggtgcatg gccggcaggc tggcgcgttc
    23881 tttcacggtt actacgggga ttattgtttc ctgccgcttt aggtgttttg cggggagcaa
    23941 ttgctggtgt cgtatttgcg cccgggcaac gtgggcgcgg cccatcacgc ctgggcggtg
    24001 ttgaagctgt tggttcaacg cctccgccaa gtctggccgc gggtgaagat cattgttcgg
    24061 ggggatgcgg ggttctgctg ctggcgtagg ttgcggtggt gcgaacaaaa caaggttcat
    24121 tacctcatcg ggctggtcaa aaacgcgcgg ctgcaggcac tggcccaacc gctcatcgag
    24181 caagcggaac aggcgttcct gcaaacgcag gagaagcaac gacacttggg cgaagtcaat
    24241 tatgccgcga cacctgggac aaggagcggc gggtgttggt caaggccgag cacactgccc
    24301 gggggagcta tccgcgtttt gtggtgacca atcttgaagg ggcagcgcag accctctatg
    24361 acgaggggta ttgcgcacgg ggtgagatgg aaaaccggat caaagaaccg ccattgggat
    24421 tgtttgccga ccggacccgt tgcggcggct ggtgggccaa tcagttccgg ctgctcttga
    24481 gccgttgcgg ctacgtgctg ctggagcggt tgcgggcact gggcttggcg gggacggaac
    24541 tggcgcgggc gcaagcgggc acgattcgct tgaaactctt gaagattggc gcggtggtgc
    24601 tgcgcaacac ccggcgggtg aaactgctgc tctccagcgc ctatccgtat cagaaaatct
    24661 tcggccaggt gttccg
//
LOCUS       DAOPQG010000052        24604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2569_length_24604_cov_14.958165, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000052 DAOPQG010000000
VERSION     DAOPQG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 24604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24604
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_24604_cov_14.958165"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1458
                     /locus_tag="PKI07_07645"
     CDS             244..1458
                     /locus_tag="PKI07_07645"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOC50964.1"
                     /translation="MKILVLNSGSSSIKFQLREDHGGVVLSKGLIERIGEPLGAIIIS
                     TANGRKIRKELELPQHTVALQHFFELLPSPELQCLGSLKEIGAVGHRVVHGGEEYSDS
                     VLIDDKAIATIDRLSSLAPLHNPPNLKGILACRELLPGVPQVAVFDTAFHQTMPPVAY
                     MGALPRELYDKYKIRQYGFHGTSHRYVHQRACLLAGLEGQPSKIATAHLGNGCSITAI
                     KDGKVIDTSMGFTPLGGLVMGTRPGDIDAALPCFLHDQGMECREIQHMLQKKSGLMGL
                     SAGLSNDMRVLLEKSATNEYARLAVDVFCYRLKKYIGSYAAALGGLDVLAFTGGIGEN
                     AHPIRARACEGLEFLGIQIDPQRNAAGSGRETLISTDAARVKVYVIPTDEEYVIAEET
                     LRLAGGNSRPAA"
     gene            1492..2484
                     /gene="pta"
                     /locus_tag="PKI07_07650"
     CDS             1492..2484
                     /gene="pta"
                     /locus_tag="PKI07_07650"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041734229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOC50965.1"
                     /translation="MHFTEELLRKAQQRNKCIVLPEADDDRTLQAADRLTRDGICRVV
                     LTGDPGQIQASARKAGADVSRCVILSPADETEAAPLAQELHLLRKNKGLTLEAARELI
                     KDPLYFATMLLKVGRVDGYVAGATHTTAENLRPALQIIKATPGLRTVSSFFLMVFPEA
                     KWGHQGVLLYSDCGMVEQPSAEQLADIAIASARTFRQLVGAEPRVAMLSYSTKGSANS
                     PDTRKVVKAVELAKEMAPEIVLDGEMQADAALIPSIGERKCRGSPVAGKANCLIFPDL
                     DAGNIAYKLTERLGGAQALGPLVQGMAKPAHDLSRGCSADDIVKVAAISAIMAD"
     gene            complement(2593..4005)
                     /locus_tag="PKI07_07655"
     CDS             complement(2593..4005)
                     /locus_tag="PKI07_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC50966.1"
                     /translation="MFSGRLELLRRNIGVRLSLLYALIFTVGSAALLALAYYLLAAAV
                     GSKDREVLDARLKEAAVVYEVGGVVALRSWVYNQPIELQNSMLVHLVNPRTRTDLVIS
                     APEAWIGLRDASGWDGLVQTPYLRIPQNAERDYTLGRLELPDGRILQIGHITNSRAAL
                     LNPIRRSFLLAGSLTVLLGFLAGALFAHRAMRPVRQIVATARSIIRTGQLDARVPVRD
                     ATDEFDELVRLFNTLLDKNQALIRALRESLDNAAHDLRTPLTRLRGVAENALQAGADP
                     ATAREALADCLEESERVLSMLNALTDIAEAEAGMMKLQREPTDLGQLAREAAELYQYV
                     AEEKPVAVHLEIPQPCEAEVDRTRMRQVFANLLDNALKYTPAGGSVTIGVREEPGHAV
                     ARFRDTGIGIPSGEQDKIWARLYRGDKSRSQRGLGLGLSLVKAIVEAHGGRVSVSSQA
                     GEGSEFTVSLPRHGPPVSRE"
     gene            complement(3986..4654)
                     /locus_tag="PKI07_07660"
     CDS             complement(3986..4654)
                     /locus_tag="PKI07_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC50967.1"
                     /translation="MRILVVEDDRKIASFVVKGLKESGYAVDHFADGEQGLIMAQTIA
                     YDAAIVDLMLPQLDGLSLIRQLRARGKRMPVLILSAKASVDDRVKGLQAGGDDYLTKP
                     FAFSELLARVQALIRRATQVAEPTRLVVGDLTLDLLNRQVERGGERIVLQPREFALLE
                     YLMRNAGRVVTKTMILEHIFDYSFDPQTNIVDVLVHRLRSKVDKGRAMLHTLRGVGYV
                     LRPA"
     gene            complement(4663..6264)
                     /gene="nadB"
                     /locus_tag="PKI07_07665"
     CDS             complement(4663..6264)
                     /gene="nadB"
                     /locus_tag="PKI07_07665"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOC50968.1"
                     /translation="MKHFDFLVLGSGIAGLSFALKVAPHGRVAIITKKDRTESNTNYA
                     QGGIAAVTSKEDSFELHVRDTLEAGVGLCKEPAVRTIIEEGPARIAELIELGMHFTER
                     EIPSAPGSRELDLGREGGHSKRRILHAKDVTGREIERALLAAVARQPRITLFENHFAI
                     DLISSQKLGHPGDNQCLGVYVLDKQSGQVETFVAPVTLLATGGLGKVYLYTTNPDIAT
                     GDGVAMAWRAGAAVANMEFVQFHPTCLYHPRAKSFLISEAVRGEGAVLKNLAGVEFME
                     QVHPLKSLAPRDIVARAIDSEMKRSGADHALLDITHKPARFLMERFPNIYQICLGYGI
                     DMTKEPIPVVPAAHYQCGGVLTNVNGETEIRGLYAVGEVACTGLHGANRLASNSLLEA
                     LVCAHRAAQKAIARPASPGEFRIPPWHSGHAHNPDEMVVVAHNWGEIRRLMWDYVGIV
                     RTNKRLQRAQTRIANLQEEIQEYYWDFIVTSDLLELRNIATVAALIVASALRRPESRG
                     LHYNLDYPERNPDWARRDTILRREF"
     gene            complement(6265..7131)
                     /gene="panC"
                     /locus_tag="PKI07_07670"
     CDS             complement(6265..7131)
                     /gene="panC"
                     /locus_tag="PKI07_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOC50969.1"
                     /translation="MQIVRSVAAMQRLGRSWQRAGARVGLVPTMGCLHDAHLSLVRRA
                     RRAVGPRGRVVVSIYVNPTQFAPGEDFARYPRDLARDCGLCRAAGVDVIFAPSDRQMY
                     ARGADGDFSTFVSEESLARGMEGAARPTHFRGVTTVVAKLFHLVQPEVAIFGAKDYQQ
                     AAIIKRMVRDLNFPVKIIVAPIRRERDGLAMSSRNRYLTGDLRRQAVVLWQALQRARA
                     AVRPPGKTIPAARLKAALRRFIARTPAARLDYMEFFDPATLTPVAKVRRGVHLALAVF
                     VGGTRLIDNTRL"
     gene            7219..8349
                     /locus_tag="PKI07_07675"
     CDS             7219..8349
                     /locus_tag="PKI07_07675"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOC50970.1"
                     /translation="MRLRILIIPDKFKGTLTARAAAEAIAGGWRQERPQDHLDLLPMS
                     DGGDGFGEVIRALLGARRRRVRTVDAAHRPCAAAWWWEPRTRTALIESANVIGLAMLP
                     PRQFHPFHLDTRGLGAVVRAAAQQGAARCLIGIGGTATNDGGFGLARALGWQFHDRHG
                     RLIERWVDLWRLDFLRPPRRRRWFTRKRVATDVQNPLLGPRGASRVYGPQKGLRPQDF
                     LLAERCLGRLARVVRQQFGHDLAREPGAGAGGGLGFGLRAFLGAELQPGFDLFARHAS
                     LQRRLRAADLVITGEGAIDRSSLMGKGVGQIAQHCRDLNLPCIGLGGRVRVGPEARRL
                     FTQIYALTDLASLRQAQAQPAFWLAKLARQSARSVSLRPLRS"
     gene            complement(8462..9847)
                     /locus_tag="PKI07_07680"
     CDS             complement(8462..9847)
                     /locus_tag="PKI07_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC50971.1"
                     /translation="MNNTSSTPEPPRSAASTPKLSRREFVAGGMAAAATTALAGVGIP
                     WVHAGEDNTIRLALIGCGGRGCGAVADAFDSPRGPVKLTVMADLFENRLNSALQSLSE
                     KFADRVEVPPERRFTGFDAYRKAIDCLRPGDVAMLTGYSAFRPMQLEYAVAKGVNVFM
                     EKSFAPDAPGLRRLIRAGEAAEKKNLKIAAGLQCRHSRNRQELIRRLRAGELGEVQLI
                     RAYRMQPVGHLGRKPAGEKELFWQIRNFVHFLWVSGGLFAEMNIHQIDELCWIKDQWP
                     VSAHGVGGRSAQNTDGSQNLDSLSIEWTFPDGTKALDVVRWLPKCHEEFATFIHGTKC
                     AAQFSGWSHLGTVHIYKDQRCRPENIAWRAPKEAATPWQEEWTALLDAIRNDKPHNET
                     VRAAHSNIADLMGRAAVHTGQVVTWEQMMASNFQLCPNIDELNENSPPPILPDDQGRY
                     PVPVPGQWTEV"
     gene            complement(9971..11602)
                     /locus_tag="PKI07_07685"
     CDS             complement(9971..11602)
                     /locus_tag="PKI07_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOC50972.1"
                     /translation="MKIRNPLSPKTVYLVCSGDLRLSANQQCWPEQARMEAALARALK
                     AEGWKVLRAHAYDPQTRHGFIDSQRRGIEVFRKLDPQAPLIVAESVWQYSHHVLPGLT
                     THRGPILTVANWSGTWPGLVGMLNLNGSLTKAGVRYSTLWSERFQDDFFRAGLRQWLQ
                     TGVVTHDQSHVKDLRLLKLPAADERLGQEAARRFRQQKAILGVFDEGCMGMFNAIIPD
                     ELLHPTGVFKERLSQSTLYAAMQQVSEAEARAVLDWLLKQGMTFQWGREEETQLTEAQ
                     TLQQCRMYVAAVRLADEFGCDAVGIQYQQGLKDLVPASDLVEGLLNNVDRPPVTAAGS
                     RRVLFAGEAVPHFNEVDECAGLDGLVTYRLWRELGFAPENTLHDVRWGEAFKGNGVND
                     FVWVFLISGAVPPAHLRGGYRGATSHRQPPMYFRLGGGSIQGVSKPGPIVWSRVFVMD
                     GKLHCDLGVGKAVELPEKESRRRLQLTTPQWPIMHAVLDGITRDQFMARHKANHIQVV
                     YAPSLKEAHRACRIKAAMLAELGLETHLCGNVALK"
     gene            12247..13083
                     /locus_tag="PKI07_07690"
     CDS             12247..13083
                     /locus_tag="PKI07_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOC50973.1"
                     /translation="MNAAGETLPALSLIASQRPLVIAHRGYCQIAPENTLPSFRFALA
                     AGADLVELDYHHSKDGKMVVLHDNYLDRTTDATARWPEKQIKVGSRTAAEIQSLDAGK
                     WFDPLFAGTRIPLLAEALDTIQAGGVTLIERKDGAPADCIKLLREKGLINKVVVQAFD
                     WEYLRVFHEQEPAQVLGALGPPAFLSDGRKPEGIAKGLSAAWLDELQKTGAKVAVWND
                     NVSREAVQLAHQRGLKVWVYTINDPAAANKLLDLGVDGIITNNTSLIWKTVALRARTG
                     NS"
     gene            complement(13187..14986)
                     /gene="aspS"
                     /locus_tag="PKI07_07695"
     CDS             complement(13187..14986)
                     /gene="aspS"
                     /locus_tag="PKI07_07695"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOC50974.1"
                     /translation="MKRTHHCNELRPAHIGRAVTLEGWVHSRRDLGGLIFIDVRDREG
                     RTQTVFDPSDLPPPLFEQAAGLRSECVVRISGKVRARPDGTNNPRISTGEVEVLASGL
                     EVLNPAEVLPFPVDEPEVAAKVNEELRLKYRYLDLRRPEMTRNLRLRSKVATATRLFM
                     DEQGFLEVETPTLFKSTPEGAREFLVPSRTNPGQFYALPQSPQQFKQILMVAGVERYF
                     QLARCYRDEDLRADRQPEFTQIDLELSFIEREDIYALIEGLLQRIWKTALNLDVPTPF
                     RRLRFEEAMNRYGSDKPDTRFGMELVDFTEEFRASQFKIFSSAVASQGVVKALNAKGL
                     ADVTQGQMDTMTEYAKAFGAKGLAYIKVEGGEWKSPIVRFFSDAEKAALTSKLGIAEG
                     DLILFAADQWLTACEILGKIRLYCAEVLKAQGKLVIPPDRFDFLWVVDFPLLSFDKEQ
                     NRWYSSHHPFTAPVPEDIPLLKSDPKKVRGQHYDIVVNGVELGGGSIRIHQAEVQKTV
                     FEDVLQIPPEEIKLRFGYLLEAFKYGAPPHGGIALGFDRLIAILCGTPSIRDVIAFPK
                     TAKGTCLMTESPSPASARQLRDLHLEVKAAKKE"
     gene            complement(15101..16390)
                     /locus_tag="PKI07_07700"
     CDS             complement(15101..16390)
                     /locus_tag="PKI07_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOC50975.1"
                     /translation="MATYFARGGTADNLRTAEKRAALAQALKVIGRPLRKVLVLPPDH
                     TRLNSDAGELTRLLYGMLAPQATVDIMPALGTHAPMTEAQLRLMFGRSIPRERFRVHD
                     WRHGLRRVGEVPGRLIREWSEGRVDYSVRIEVNQLLFAGYDLILSVGQIVPHEVVGMA
                     NYTKNVVVGAGGADIINKSHFLGAAFGMERMMGRIETPVRRLFNYGVQAFLRDLPIVY
                     ILTVMEPNPAGRGMIMRGLYVGDDMETFTLGARLSQKVNLNLLAKPLKKVVVFLDPRE
                     FTSTWLGNKAIYRTRMAMADGGELIVLAPGLQTFGEDAEIDRLIRQHGYRGTPRTLAA
                     VKAHARLRNNRSAAAHLIHGSSEGRFRITYCPGPGLSLEAIRAVGFGAAELADALKRY
                     PPDRLRDGPNRLPNGEEIFYISNPALGLWALQRQFAA"
     gene            complement(16426..17283)
                     /locus_tag="PKI07_07705"
     CDS             complement(16426..17283)
                     /locus_tag="PKI07_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC50976.1"
                     /translation="MTIPFQINLKDQVAVITGGGGVLCGAMADAVAQCGAKVAILDLK
                     LDKAQQGADRIRQAGGVARGFAANVLDKASLEQAHEQVKAALGPVDILINGAGGNHPR
                     GTTSREFLSREDLTAPQEGVVTFYDLTAEGIQFVFNLNFLGTFLPTQVFSRDMVLQGR
                     GNIINISSMNAFRPLTKIPAYSAAKAGVSNFTQWLAVHFSRTGVRVNAIAPGFFLTDQ
                     NRGLLTQPDGSLTARGTTITAHTPQGHFGKPEDLIGTLLWLLSGEGSGFVTGQVIAVD
                     GGFSAFSGV"
     gene            17395..18345
                     /locus_tag="PKI07_07710"
     CDS             17395..18345
                     /locus_tag="PKI07_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOC50977.1"
                     /translation="MISSLPKPEVIITHESDLDGLVSGVLLQRLARKLFAADVRLEAY
                     NHHNWRQRELREKSAWVADLAYEPRLDRPNWVIIDHHATALAPRNAVFIHSLNKSAAL
                     LCYELCRQQGLGSPELDRLVHLTNVGDLFLEDDPDFVLASDYASLVKHYQFWNLHALV
                     NGQIEQLLNNPLLRVMEVKRNIEDPLGFAWARENINEISPQVGFVDTIIGNTNLIVHQ
                     LLEQGATKYPVLLTLFRRANGVIIASLRSRNGQAVKVVEKLQGGGHANASAATLPRSV
                     KTIPDAITYLRQVLNPQKETPVNSLEEAFAAFDQSQKSGA"
     gene            18483..19253
                     /gene="rph"
                     /locus_tag="PKI07_07715"
     CDS             18483..19253
                     /gene="rph"
                     /locus_tag="PKI07_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOC50978.1"
                     /translation="MAPPQSATTTPVVRADGRRTNQLRPVRFQNHIAPYAAGSTLIEW
                     GNTRVICGVTVEDAVPRWMKEQAVPGGWITAEYAMLPYSTLNRKQRDSTRGKIDGRSQ
                     EIQRLIGRAMRTALDLEKLGARTLWVDCDVLQADGGTRTAAITGGYVALALAIRKLMA
                     EGKLSESPLRRAVAAVSIGIVQKQALLDLCYEEDVAAEVDLNLVMDSGGEFIELQGTG
                     EEATFSEAQLAEMLELGKAGVRELLAAQQAALAVLQAR"
     gene            19250..20404
                     /locus_tag="PKI07_07720"
     CDS             19250..20404
                     /locus_tag="PKI07_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC50979.1"
                     /translation="MKFLHLIWSNLMRRKLRTALTLLSIVVAFVLFGFLCAIRQALAG
                     GVTMAGQNRLVTRHKISIIQSLPESYKHRMERIPGVALATQESWFGGVYQDPKNFFPQ
                     MSVVPEEYLAMYPEYLLAPEQKQAWLATRTGAIVGRKTAERFQWKIGDRIPLQSSIWT
                     KSDGSRLWEFDLVGIYDGRDKETDTSLLFFRYDYFDEARVWNKGQVGWYMVQVTDPSQ
                     AAEIARRIDEEFENSAAETKTEPEGAFLQAWAKQIGDITLITATILSAVFFTILLVSG
                     NTMSQAVRERTGELGALKAMGFTSPQVLALVLAESCLLALLGGGLGLGLAWLLVARGD
                     PTGGMLPMFFFPKGDLLRGVGFSASLGVLAGIFPAWRAMRLRVADALRRM"
     gene            20411..21610
                     /locus_tag="PKI07_07725"
     CDS             20411..21610
                     /locus_tag="PKI07_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC50980.1"
                     /translation="MAGPINWLSQVASVSKFGLLSIPQRRGSVAAAVFGIAGVVAVLV
                     GVLSMGVGFQRALQSSGAPDAAIVLRSGADSEMVSGFLRESTRVIADAPGVAHTEDGP
                     LASAELYVLINLLKRSTGTDANVPMRGVEQAALRVRDQLQILDGRMFEWGRNEVIIGV
                     GAASEFVGLELGGNLRVGQREWPIVGVFSAGGGTAESEIWTDARVLQSAYKRGDTFQS
                     VHVKLSSPAAFQEFKDALTTDPRLNVKVLRQTEYYAQQSTAVTRLIRTLGYLIAFLMA
                     IGAVFGALNTMYSSVSARTREIATLRALGFGSGAVVLSVMIESLVLALIGGGVGAVGA
                     YLTFNGLHTSTMNWNSFSQVTFAFAVTPELLGQGIVWSVFIGLIGGLFPALRGARLPI
                     AAALREL"
     gene            complement(21619..21945)
                     /locus_tag="PKI07_07730"
     CDS             complement(21619..21945)
                     /locus_tag="PKI07_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50981.1"
                     /translation="MRPTKRLPGVCAECGGSFTFPAEMIGTRAPCPCCKQQTELRLAP
                     PAEESTISRATIVYSLIGIGILLAGLIAAVVGVKRFEKLAVRQKQKAAGAGITSATNA
                     PAPAKR"
     gene            22042..22716
                     /locus_tag="PKI07_07735"
     CDS             22042..22716
                     /locus_tag="PKI07_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HOC50982.1"
                     /translation="MTFLRRKFEGSPEYARVAPFVIFVALTALQGHLGEDSRYWIYLL
                     KTLVGAWLIWEMRPFVLEMRWKLSWEAVLVGVAIFAVWVGLDGWYPRLSEVTEPGWNP
                     HLRYGEGSALAWLYVVTRIVGSSLVVPPIEEVFYRSFLYRVLVKRDFRAMPLSQFHGL
                     SFVVTSAIFGLLHPDRWLAGILCGLAYQWLVIRKQRLGDAMTAHAITNFLLGLWVAWK
                     GAWIFW"
     gene            22847..24526
                     /locus_tag="PKI07_07740"
     CDS             22847..24526
                     /locus_tag="PKI07_07740"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOC50983.1"
                     /translation="MLPCLIFEDEHLLVVNKPAGLNTHAPSPYAGDGLYDWLRHREPR
                     WAALAIIHRLDKETSGVMVFAKTPQANRSLTAQFTARVVRKKYLLLTDRPARQKEFVV
                     KSTLVRRGDRYLSQPLTRGGGIAETRFRSVRMPHVPGKTADHWHWFEAEPLTGRTHQI
                     RVHAAANGIPILGDTLYGGTPAPRVCLQAVELQLKHPASGEVMQFSVPIDLPPTGIQS
                     YAAAQTKGGLLWTTASWPSLALRQATIEGEETQAYRLVHGASDGCPGWYLDRVGDFLL
                     SQSESPLTAAQQNELEHFAATLARQGAYHKLLARQPGQVQRLAASPRLVFGKPAPERF
                     AVRENGVAYELSFSEGYSMGLFLDQRDNRRRLLSRHVAAGFPLCLPPPASRQWEALNT
                     FAYTCGFSVCAARAGARVTSIDLSKRYLEWGRRNFRLNQLDPEQHNFIHGDTLDWLRR
                     LEKKKRPFDVILLDPPTFSQSKTSGIFRAEKDYEKLITAALPLLPAGGVLFASTNAAA
                     WPPEEFIAAIERAIRRARRKIIQRHYVPQPLDFPVSRAEPAYLKTVWLRVE"
BASE COUNT         4308 a         7956 c         7749 g         4591 t
ORIGIN      
        1 ggaaactccg cgccgcgcca cactaattgg tttgacgggg ccggtccggt atgcacactc
       61 tccgcggata tgattcgact cggccgaata gttttgttgg cgaggttgcc tggcctggcc
      121 ggccgaactc acggccggaa gaaccccgag ggttccttcg tcgagtccgc taatctcgcc
      181 gcgcctccgg aggcggaatc cgcccggcgc gcttttggtt tttaccggta gaacgattga
      241 tacatgaaaa tcctggtcct gaacagcggc tcttcttcca tcaaattcca gctccgtgaa
      301 gatcacggcg gcgtcgtcct gagcaaaggg ttaatcgagc gcatcgggga acccctgggc
      361 gccatcatca tcagcaccgc caacggcagg aagattcgga aagagctgga gttgccgcag
      421 cacacggtgg cgctgcagca ctttttcgag ttgctgccca gcccggagtt gcagtgcctc
      481 ggctcgctca aggaaattgg cgccgtaggg catcgcgtgg ttcacggcgg cgaggaatat
      541 agcgactcgg tcctgattga tgacaaggcc atcgccacca tagaccgcct cagctccctg
      601 gcgcccttgc acaatccccc caatctgaag ggcatccttg cctgccgcga gttgcttccg
      661 ggcgtgcccc aagtcgcggt ttttgacacg gcgtttcatc aaaccatgcc ccccgtggcg
      721 tatatgggag ccctgccgcg ggagctctac gacaagtata aaatccgcca atacggcttt
      781 catgggacca gccaccgcta cgtgcatcag cgcgcgtgcc tcctggccgg cctcgagggc
      841 cagccctcga aaatcgccac cgcgcacctg ggcaacggct gctccatcac cgccatcaag
      901 gacggcaaag tcattgacac ctccatgggg ttcacgcctt tgggcggcct ggtgatgggc
      961 acgcggccgg gcgacattga tgcggccctg ccctgcttcc tgcatgatca aggaatggag
     1021 tgccgcgaga ttcagcatat gctccagaag aagtccgggc tcatgggctt gtccgcggga
     1081 ctttccaatg atatgcgcgt cctcctcgaa aaatcggcca ccaatgagta tgcgcgcctg
     1141 gcggtggacg tgttctgcta tcgcctcaag aaatatatcg gctcgtatgc ggcggcgctg
     1201 ggcggcctgg atgtgctggc tttcaccggc gggatcggag aaaacgcgca cccgattcgg
     1261 gcccgggcct gcgaaggcct ggaattcctg ggcatccaaa ttgatccgca gcgaaatgcc
     1321 gccggctccg gccgggaaac cctgatctcc accgatgccg cccgcgtgaa agtgtacgtg
     1381 atccccaccg acgaggaata tgtcattgcc gaagaaactc tccggctggc ggggggcaac
     1441 tcgcggcccg cggcttgact tcttcccctc ccgcttgcca aagataatct tatgcatttc
     1501 actgaagaac tcctgcgcaa agcgcaacaa cggaacaagt gtatcgtttt acccgaagcc
     1561 gatgacgacc gcaccctcca ggcggcggac cgcctcacgc gggatggaat ctgccgggtc
     1621 gtcctgacag gcgaccccgg ccagattcag gccagtgccc ggaaggccgg ggctgatgtt
     1681 tcccggtgcg tcatcctgtc cccggcggat gaaacggaag ccgctccgtt ggcgcaggaa
     1741 ctgcacctgc tccgcaaaaa caaagggctg accctcgaag cggcccgcga actgatcaag
     1801 gatcccctct attttgccac catgctgctc aaggtggggc gcgtggacgg ctacgttgcc
     1861 ggggccaccc acacgaccgc cgaaaacctg cggcccgccc tccaaatcat caaggctact
     1921 cccggcctgc gaacggtgtc gtcgttcttc ttaatggtgt ttccggaggc caaatggggt
     1981 catcaaggcg ttttgctcta ttcggattgc ggcatggtcg agcaaccttc cgccgagcag
     2041 ttggcggata tcgcgatcgc ctccgcgcgc acgttccgtc aactggtcgg cgccgagccc
     2101 cgggtggcga tgctctctta ttccaccaaa ggctccgcca attctcccga tacccggaag
     2161 gtcgttaaag ccgtcgaact ggccaaggaa atggccccgg agattgtcct ggacggggaa
     2221 atgcaggccg atgcggcctt gatccccagc atcggcgagc gcaagtgccg gggttcgccg
     2281 gtggcgggaa aagccaattg cctgatcttc cccgacctcg atgccggcaa catcgcctac
     2341 aaactgaccg agcgcctcgg cggcgcccag gccctcggcc cgctggtgca aggcatggcc
     2401 aaacccgccc acgacctctc gcgcggctgc tccgcggatg acatcgtcaa agtcgccgcc
     2461 atttccgcca tcatggccga ctagccaggc agcgacgctc cccgggcttc cggcccccgt
     2521 cccggagccg ggggcaaggc cggcgtgaag ccggcgcggg cgcccgcgcc tccgggggct
     2581 tcgcgtttcc cctcactccc gggaaacggg cgggccgtgc cgcggcaggc tgacggtgaa
     2641 ctccgatccc tcgccagcct ggctggaaac gctgacgcgg ccgccgtggg cctcgacaat
     2701 ggctttgacc aggctcaggc ccagacccag gccacgctgc gagcgactct tgtcgccgcg
     2761 gtaaaggcgc gcccagatct tgtcctgctc cccgctgggg atgccgatgc cggtgtcacg
     2821 gaagcgcgcg acggcatggc cgggctcttc ccgcacgcca atggtgacgc tgccgccggc
     2881 cggtgtatat ttaagggcgt tgtcaaggag gttggcaaaa acctgccgca tgcgcgtgcg
     2941 gtccacctcc gcttcgcagg gctgcgggat ttcgaggtgg acggccactg gcttttcctc
     3001 cgcaacgtat tgataaagct cggccgcttc ccgcgccaac tgccccagat cggtgggctc
     3061 gcgttgcagc ttcatcatgc cggcttcggc ctcggcgatg tccgtgagcg cattgagcat
     3121 gctcagcacg cgctcggatt cttccaggca atccgccagc gcctcgcggg cggtggccgg
     3181 atcggccccg gcctgcaaag cgttctccgc cacgccgcgc aaccgcgtca gcggcgtccg
     3241 caaatcatgg gcggcattgt ccagcgattc gcgcaaggcg cggatcaggg cctggttctt
     3301 gtcgagcagc gtgttgaaga ggcgcaccag ttcgtcgaac tcgtccgtgg cgtcgcgcac
     3361 gggcacccgg gcatccaact ggccggtgcg aataatggag cgggcagtcg cgacaatctg
     3421 ccgcacgggc cgcatggcgc ggtgcgcaaa gagggccccg gccaggaaac cgagcaggac
     3481 ggtgaggctg ccggcgagaa ggaagctgcg gcggatgggg tttaagaggg cggcgcggct
     3541 gttggtgatg tggccaattt gcagaatccg gccgtcggga agctccagcc gccccagggt
     3601 gtagtcgcgc tccgcatttt gcggaatgcg caggtagggc gtttggacca ggccatccca
     3661 gccggatgcg tcgcgcagtc cgatccaggc ctcgggggcg ctgatgacca ggtccgtccg
     3721 ggtgcgcggg ttgaccaggt gcacgagcat ggagttctgc agttcgatcg gctggttata
     3781 cacccaactg cgcaacgcaa cgactccgcc cacctcataa accaccgccg cctccttgag
     3841 gcgcgcatcc agcacttccc ggtctttgct gccgacggcg gcggccagca ggtagtaggc
     3901 cagcgccagc agggccgcgc tgcccaccgt aaagatcagc gcgtagagca ggctgagccg
     3961 aaccccgata ttccgccgca gcagttcaag ccggccggag aacataacca accccgcgca
     4021 gggtatgcag catcgcccgg cccttatcca ccttgctgcg caggcgatgc accagcacat
     4081 ccacgatgtt ggtctgcggg tcgaagctgt aatcaaagat gtgctccaga atcatggtct
     4141 tggtgactac gcggccggcg ttgcgcatga gatattccag cagcgcaaat tcgcgcggct
     4201 gcaaaacaat gcgctcgccg ccgcgctcga cctggcggtt gagcaagtcc agggtcaggt
     4261 cccccaccac cagccgggtc ggctccgcca cctgggtggc ccggcggatc agcgcctgca
     4321 cgcgcgccag cagctcggag aacgcgaaag gcttggtcag gtagtcgtcg ccgcccgcct
     4381 ggaggccctt gacgcggtca tccaccgagg ctttggcgct cagaatcagc acgggcatgc
     4441 gcttgccccg ggcgcgaagc tgccggatca ggctcagccc gtcgagctgc ggcagcatga
     4501 gatccacaat cgcggcgtcg tacgcgatgg tttgcgccat gatgagcccc tgctccccgt
     4561 ccgcgaagtg gtccaccgca tagccgcttt ccttcagccc cttgaccaca aaggaagcaa
     4621 tcttcctgtc gtcttccacc accagaatac gcatacgccc ggtcagaact ctctccgcaa
     4681 aatcgtgtcg cgccgggccc agtcgggatt ccgctccggg taatccaggt tatagtgcag
     4741 cccgcggctt tccggccgcc gcagcgcgct ggcgacaatc aacgccgcca ccgtggcgat
     4801 attgcgcagt tccagcaggt cgctggtcac gatgaaatcc cagtaatact cctggatttc
     4861 ctcctgcagg ttggcgatgc gcgtctgcgc gcgttgcagg cgcttgttcg tccgcacaat
     4921 ccccacgtag tcccacatca gccggcggat ttccccccag ttgtgcgcga ccaccaccat
     4981 ctcgtccggg ttatgggcgt ggccggaatg ccagggcggt atccggaact ccccgggact
     5041 agcgggccgc gcgatggcct tttgggcggc gcggtgggcg cagaccagcg cttcgagcaa
     5101 cgaattgctc gccagccggt tggccccatg cagcccggtg caggccactt cgccaaccgc
     5161 atacaaaccg cgaatctccg tctcgccgtt gacgttcgtc agcacgccgc cgcattggta
     5221 atgcgcggcg ggcaccaccg ggatgggctc cttggtcatg tcaatgccat aaccgaggca
     5281 gatctggtaa atgttcggaa accgctccat gagaaaccgg gccggcttgt gcgtgatatc
     5341 gagcagggcg tggtccgcgc cgcttcgctt catctcgctg tctatggcgc gcgcgacgat
     5401 atcgcggggc gccagggatt taagcggatg aacctgctcc atgaactcca cgcccgcaag
     5461 gttcttgagc acggcgccct cgccgcgcac cgcctcgctg atcaggaacg acttggcccg
     5521 cggatgatac aggcaggtgg ggtgaaactg gacaaactcc atgttggcca ccgccgcccc
     5581 cgcgcgccag gccatcgcca cgccgtcgcc ggtggcgata tcggggttgg tggtgtacaa
     5641 atagaccttg cccagtccgc cggtggccag gagggtgacc ggcgcgacaa acgtctccac
     5701 ctgcccggat tgcttgtcca gcacgtaaac ccccaggcat tgattgtccc cggggtgacc
     5761 gagcttctgg ctgctgatga gatcaatggc aaagtggttc tcgaagagag taatgcgggg
     5821 ctggcgggcc accgccgcca gcagggcccg ctcgatctcc cgcccggtca catccttcgc
     5881 gtgcagaatc cggcgcttgg aatggccgcc ctcccggccg agatccagct cgcgcgatcc
     5941 cggcgcgctg ggaatctcgc gctcggtaaa gtgcatgccc agctcaatca actcggcaat
     6001 ccgcgccggg ccctcctcga taatcgtgcg cacggcgggc tccttgcaca gcccaacccc
     6061 ggcttccagc gtgtcgcgca cgtgcagctc gaaggaatcc tccttgctgg tcaccgccgc
     6121 aatgccgccc tgcgcgtagt tggtgttgga ctccgtgcga tcctttttgg tgatgatcgc
     6181 cacgcgcccg tggggcgcca ctttcagcgc gaaggaaagc ccggcaatgc cgctgccgag
     6241 caccagaaaa tcaaaatgct tcatttacaa ccgggtattg tcaatcaatc gggtgccgcc
     6301 cacaaacacc gccagcgcca ggtgaacgcc gcggcgcacc ttcgccaccg gcgtgagcgt
     6361 cgccgggtcg aaaaactcca tgtaatccag ccgcgcggcg ggggtccgcg cgatgaaacg
     6421 cctcagcgcg gctttaagcc gcgccgccgg gatcgtcttc cccggcggcc gcaccgccgc
     6481 ccgcgcccgt tgcagcgcct gccagagcac caccgcctgc ctccgcaggt cgcccgtgag
     6541 ataccggttc cgcgagctca tcgccagccc gtcgcgctcc cgccggatgg gcgccacgat
     6601 aatcttcacc gggaagttca ggtcgcgcac catgcgcttg ataatggcgg cctgctggta
     6661 atccttggcg ccaaaaatcg ccacctccgg ctgcacgagg tgaaataact tcgccaccac
     6721 cgtggtcacc ccgcggaaat gcgtgggccg cgccgcgcct tccatccccc gcgccaggct
     6781 ttcctcgctc acaaaggtgc tgaaatcccc gtcagcccca cgcgcataca tctgccggtc
     6841 gctcggggca aaaatcacat ccacccccgc cgcgcggcac aggccgcagt cgcgcgccag
     6901 gtcgcgcgga taccgggcaa aatcttcgcc cggggcaaac tgggtcggat tgacgtagat
     6961 gctcaccacc acccgcccgc gcggcccaac cgcgcggcgc gcccgccgga cgaggctcaa
     7021 atgcgcgtcg tgcaggcagc ccatcgtcgg gaccagcccc acccgcgcgc ccgcccgctg
     7081 ccagcttcgc cccaggcgct gcatggcggc tactgacctc actatctgca tgacgtaaat
     7141 gtgcggcagg cggaaatcag tggcaacccc ataaacagcc tttatcttcc cgcccgcctg
     7201 cctttatcct ccacctccat gcggttgcgc atcttgatta ttcccgacaa attcaagggc
     7261 accctgaccg cccgcgccgc cgcggaggcc atcgccggcg gctggcgcca ggaacgcccg
     7321 caggatcacc tggacttgct gcccatgagc gacggcggag acggctttgg cgaagtcatc
     7381 cgcgccttgc tcggcgcccg ccggcggcgc gtccggaccg tggatgccgc ccatcgcccc
     7441 tgcgccgccg cctggtggtg ggaaccgcga acccgaaccg ccctgattga gtcggcgaac
     7501 gtcatcggcc tggccatgct gccgccgcgg caattccatc ccttccacct cgacacgcgg
     7561 ggcctgggag cggtcgtgcg cgccgccgcg cagcaagggg cggcgcgctg cctgattggc
     7621 attggcggca ccgcgaccaa cgacggcggc ttcggcctgg cgcgcgcgct gggctggcaa
     7681 ttccacgacc gccacggccg gttaatcgag cgttgggttg acttgtggcg gctcgacttc
     7741 ctccgccccc cgcggcgccg ccgctggttc acccggaaac gggtcgccac cgacgtgcaa
     7801 aaccccctgc tcggcccgcg cggcgccagc cgggtttatg gaccccaaaa gggcttgcga
     7861 ccgcaggatt tcctcctggc cgaacgctgc ctgggacgcc tggcccgcgt cgtccggcaa
     7921 caattcggcc acgacctggc ccgggaaccc ggggcgggcg cgggaggcgg gctgggcttc
     7981 ggactgcggg ctttcctggg cgcggagctg cagccgggat ttgatctgtt cgcgcggcac
     8041 gcgagcctgc aacgacgttt gcgcgcggcg gaccttgtca ttaccggcga gggcgcgatt
     8101 gaccgctcat cgctgatggg caagggcgtg gggcaaattg cgcagcattg ccgggacttg
     8161 aaccttccct gcatcggcct gggcggcagg gtcagggtcg gcccggaggc ccgccgtctt
     8221 ttcacgcaga tatacgcgct caccgacctg gccagcctcc gccaagccca ggctcagccg
     8281 gccttttggc tggccaaatt ggcgcgccaa tcagcgcgtt ccgtgagcct gcggcctctc
     8341 cgctcctgac cgcaagccgg gcgcccggcc gcgccgcgac ccgaggctca gggatgcctg
     8401 cggcgggaag cccgattccc gggccattcc ccgccatccg cgcgccttcg ggttgctccg
     8461 cttacacttc cgtccactgc ccgggcaccg gaacgggata gcgcccctgg tcatcgggca
     8521 gaatgggcgg gggactgttc tcgttgagtt cgtcaatatt ggggcagagc tggaagttgg
     8581 aagccatcat ctgctcccag gtgacaacct ggccggtatg caccgcggcg cggcccatga
     8641 gatcggcgat gttcgaatga gccgcgcgca cggtctcgtt gtgcggcttg tcgttgcgga
     8701 tggcatcgag cagggccgtc cattcctcct gccacggcgt ggcggcctcc ttcggagcgc
     8761 gccaggcgat gttctccggg cggcagcgct ggtctttgta aatgtggacg gtgcccaggt
     8821 ggctccaccc cgaaaactgg gcggcgcatt tcgtgccatg aataaaagtg gcaaactctt
     8881 cgtgacattt cggcagccag cgcaccacgt ccagcgcctt ggtgccgtcg ggaaaagtcc
     8941 attcaatgct gagcgaatca agattctggc tgccatcggt gttctgcgcc gaccgccccc
     9001 cgaccccatg cgcgctcacg ggccattgat ccttgatcca gcacagctcg tcaatctggt
     9061 gaatgttcat ttcggcaaac aaaccgcccg aaacccagag gaaatgcacg aagttccgga
     9121 tttgccagaa caactccttc tcccccgccg gcttccggcc caggtggccg accggctgca
     9181 ttcgataagc ccggataagc tgcacctcgc ccaactcgcc ggcccgcaat cgccggatca
     9241 gttcctggcg attgcgcgag tggcggcatt gcagcccggc ggcaatcttg aggttcttct
     9301 tctccgcggc ctccccggcc cggatcaggc gccgcaaccc cggcgcgtcc ggcgcaaacg
     9361 atttctccat gaacacgttg acccccttcg ccacggcgta ttcgagctgc atgggccgga
     9421 aggccgaata gccggtgagc atggccacat cgcccggacg gagacagtca atggccttgc
     9481 gataggcatc aaaaccggta aaacgccgct cgggcggcac ctcgacccgg tcggcaaatt
     9541 tctcgctgag cgactgcagc gccgaattga gccggttctc aaacaaatcg gccatcacgg
     9601 tcagcttgac cggcccgcgc ggcgaatcaa aggcgtcggc caccgcgccg catccccgcc
     9661 cgccgcagcc gatgagggca aggcggatcg tattgtcttc cccggcgtga acccagggga
     9721 tgcccacgcc ggccagggcg gtcgtggccg ctgccgccat gccgccggcg acaaattcac
     9781 ggcgggaaag tttcggggtg ctggccgccg aacgcggcgg ttccggtgtg gatgatgtgt
     9841 tgttcatata aatcgttcag tggcggacgc gcctctccgg ctccgccggc tcccggcccg
     9901 cctgcgcgct tgcgccgcgg ccccgctttt ctgccgcccg ccgccgggcc gttcgccgcc
     9961 cggcgccctt ttatttcagg gcgacgttcc cgcacaagtg cgtttccagc cccaattccg
    10021 ccagcatggc ggccttgatg cggcacgcgc ggtgcgcctc tttgagactg ggcgcataaa
    10081 ccacctggat atggttggcc ttgtgccgcg ccataaactg gtcccgcgtg atgccgtcga
    10141 gcacggcatg cataatgggc cactgcggag tcgtcaattg caaacggcgc cggctctctt
    10201 tctccggcag ctcaaccgcc ttgcccaccc ccaggtcgca atgcagtttt ccgtccatga
    10261 cgaacacgcg gctccagaca atcggcccgg gcttggaaac cccctggatg gagccgccgc
    10321 ccaggcggaa atacatgggc ggctggcgat ggctggtggc gccccggtag ccgccccgca
    10381 ggtgcgcggg cggcaccgcg cccgaaatca gaaacaccca gacaaaatcg ttcaccccgt
    10441 tgcccttaaa cgcctcgccc cagcggacat catgcaaggt gttttcgggc gcaaacccca
    10501 actcgcgcca cagccggtag gtcaccagcc cgtccaggcc ggcgcattcg tccacctcgt
    10561 taaaatgcgg aacagcctcg cccgcaaaca gcacgcgccg gctcccggcg gccgtcaccg
    10621 gcggacgatc cacgttgttc agcaacccct ccaccaggtc gctggccggc accaaatcct
    10681 tgaggccctg ctgatactgg atgcccacgg cgtcacagcc aaattcatcc gccagccgga
    10741 cggcggcgac atacatcctg cactgctgca gcgtttgcgc ctcggtcaac tgcgtttcct
    10801 cctcgcggcc ccactggaag gtcatcccct gcttgagcag ccagtccagg accgcgcgcg
    10861 cctccgcctc gctcacctgc tgcatcgccg catacagggt ggattggctc agccgctcct
    10921 tgaacacccc cgtcggatgc aacaattcgt cgggaataat cgcgttgaac atccccatgc
    10981 agccttcgtc aaagaccccc aggattgcct tttgctggcg gaaccggcgg gcggcttcct
    11041 ggcccagccg ctcgtccgcc gccggcagct tgagcaggcg caaatccttg acgtggcttt
    11101 ggtcgtgcgt caccacgccc gtctgcagcc attgccgcaa acccgcgcgg aaaaagtcat
    11161 cctgaaaccg ctcgctccac agcgtgctgt agcgaacccc ggccttggtc agcgagccgt
    11221 tcaggttcag catccccacc agcccgggcc aggtcccgct ccagttggcc accgtcagaa
    11281 tcggcccccg gtgcgtcgtc agcccgggca gcacatgatg gctgtattgc cagacggact
    11341 cggccacgat caggggcgct tgcggatcga gcttgcggaa cacctcaatc ccccgccgct
    11401 gcgaatcaat aaaaccatgc cgcgtctgcg gatcgtaagc atgcgcgcgc aacaccttcc
    11461 agccctccgc tttgagcgcc cgggcaaggg cggcttccat ccgggcctgc tccggccagc
    11521 attgctggtt ggccgaaagc cgcagatcac cgctgcagac caggtaaaca gttttggggg
    11581 agagaggatt cctgattttc atgttctgga tatagctcta tgactctgcg gcctttttta
    11641 gcacatcgcc tcccccaagt cactgaatct ttttgcccta cccgcgccat aataacaccc
    11701 aaccccaccc gagcccaaag atagcccctc cgccaggctc ccttcccccc gccgaaacct
    11761 tcccaacaat accgatgctc cttccgtcct taagcgctgt gtacaagggg tgtacaaggg
    11821 gtgctcaccg ggagttcata ggattttagc tggtacaccc gatgggcatc ccttgtacac
    11881 cccttgtaca cggcttaggg ttagctattg atacgcctga aatgagagga gttgaagtgg
    11941 tttacgggaa accgcgggtt taggaagtgc tggagggctt ggaagtctct atctgaaggt
    12001 gtccaaaaat tggacacttg gcttggtggt tgaggttggg aatctttgat ttttggagaa
    12061 ttgtgggagc cgcgctgtgg cgggctatta agcggtgccg gtggagtctt tttgaagggc
    12121 ttatttgcgc ttgcttccac ggtgcggctg acgcatggtg cctgcgtgca tataaagccc
    12181 gtcttctcct ggaaaagcct gtttaacctc cccggaacgg ccggtttggt ttccctcact
    12241 atgattatga atgccgcggg tgagacgctc ccggcgttga gccttatcgc cagccaaagg
    12301 ccgctggtta ttgcccatcg cggctattgc cagattgcgc cggaaaacac gctgccgtcg
    12361 ttccggttcg ccctggcagc cggggcggac cttgtggaac tggattatca ccatagcaag
    12421 gatggcaaaa tggtggtcct ccacgataat tatttggatc gcacgaccga cgcgacggcc
    12481 cggtggccgg aaaagcagat caaggtggga agccggacgg cggcggaaat acaaagcctg
    12541 gacgccggca aatggtttga tccgctgttt gccgggacgc gcattcccct gctggcggag
    12601 gcgcttgata cgatccaggc cggcggggtg accctgatcg agcggaagga tggcgctccg
    12661 gccgattgca tcaaattgct gcgcgagaag gggctgatta acaaggtggt ggtgcaggca
    12721 tttgattggg agtatttgcg ggtttttcac gagcaggagc ccgcgcaagt gctcggggcg
    12781 ctcggcccgc cggcttttct gtccgacggc aggaaacccg aaggcatcgc caaaggactg
    12841 agcgccgcgt ggctggatga gctgcagaaa accggggcga aagtcgcggt ctggaacgat
    12901 aatgtttccc gtgaagccgt gcagttggcg caccagcgcg ggctgaaggt gtgggtttat
    12961 accattaacg acccggcggc ggccaacaaa ctgctcgatc tcggcgtgga tggcatcatc
    13021 accaacaaca cgtcgttgat ctggaagacg gtcgcgctgc gcgcgcggac cgggaattca
    13081 tagtgcagcc gcgtttgcgc gcggggcggc gccgccgcgg cccaagccgg ctgcctcggg
    13141 cagttttcgc gtgagagccg cgcgccgcca gactggccgg cggcgcttac tcttttttcg
    13201 cggccttgac ttcgaggtgc aggtcgcgca actggcgcgc cgaagccggg gaaggcgatt
    13261 cagtcatcaa gcacgtgccc ttggcggtct tggggaaggc gatgacgtcg cgaatgctcg
    13321 gcgtgccgca taagatcgca attagccggt caaacccgag ggcgatgccg ccatgcggcg
    13381 gggcgccata cttgaaggcc tccagcaggt agccgaagcg cagttttatt tcctccggcg
    13441 ggatttgcag cacgtcttcg aacaccgttt tctgcacctc cgcctggtgg atacgaatcg
    13501 agccgccgcc caattccacc ccgttcacca cgatgtcgta gtgctggccg cgaacctttt
    13561 tggggtcgct cttgagcagc gggatatcct ccggcaccgg cgccgtgaag ggatgatggc
    13621 ttgaatacca gcggttttgt tccttgtcga agctcagcag cgggaagtcc accacccaga
    13681 ggaagtcaaa ccggtcgggg gggatgacca gtttgccctg ggctttcagc acttcggcac
    13741 agtaaaggcg aatcttgccg aggatttcgc aggccgtcag ccattggtcg gcggcaaaga
    13801 ggatgagatc gccttccgcg atgccgagct tgctggtcag cgccgctttt tcggcgtcgc
    13861 tgaagaaccg cacgataggc gacttccatt cgccgccttc gaccttgatg taggccagcc
    13921 ccttggcgcc gaacgctttg gcgtattcgg tcatggtgtc catctgcccc tgggtgacgt
    13981 ccgccaggcc cttcgcgttg agcgccttga caacgccctg gctggcgacc gcgctgctga
    14041 agatcttgaa ctggctggcg cggaactctt cggtgaagtc caccagttcc atgccgaagc
    14101 gcgtgtcggg cttgtcgctg ccgtagcggt tcatggcttc ctcgaagcgg aggcgccgga
    14161 agggcgtggg cacgtccagg ttcagcgcgg tcttccagat gcgctgcagc aggccttcga
    14221 tcagggcgta gatgtcttcg cgctcgatga acgacagttc gaggtcaatc tgggtgaact
    14281 ccggctggcg gtcggcgcgg aggtcttcat cccggtagca gcgggcgagc tgaaagtatc
    14341 gctcgacgcc ggccaccatg aggatttgct tgaactgctg cggggactgc ggcagcgcgt
    14401 agaactggcc cgggttggtg cggctgggga ccaggaactc gcgcgcgcct tccggggtgg
    14461 acttgaagag cgtgggggtt tcgacttcga ggaagccctg ttcgtccatg aacaggcggg
    14521 tagccgtggc gaccttgctg cgcaggcgga ggttgcgggt catttccggc cggcgcagat
    14581 cgaggtagcg gtatttgagg cgcagctcct cgttcacttt ggcggccact tccggctcgt
    14641 ccaccggaaa gggcagcact tcggcgggat tcaggacttc cagcccgctg gcgaggactt
    14701 cgacttcccc ggtcgaaatc cgggggttat tcgtgccgtc cgggcgcgcc cgcactttgc
    14761 cgctaatgcg gaccacgcat tcgctgcgca gcccggccgc ctgttcaaac agcggcgggg
    14821 gcaggtccga gggatcaaac acggtttgcg tgcgcccttc gcggtcgcgc acatcaataa
    14881 agatcaggcc gcccaggtcg cgccgcgagt ggacccagcc ttcgagcgtc acggcccggc
    14941 cgatatgcgc cgggcgcagt tcattgcaat gatgcgttcg tttcatgttg cgctggaacc
    15001 ccggaggcgg aaaaccgaat cggacaaccg ttccgccggg ttcaaaagcg aggcgggatg
    15061 ctgcctggca ccagggcaat ttgaaagcca aatcttgcgc tcacgcggcg aattggcgtt
    15121 ggagcgccca caatcccagc gccgggttgc tgatgtagaa gatttcctcg ccgttcggca
    15181 ggcggttggg gccgtcgcgc aggcgatccg gcggataccg cttgagggca tccgcaagct
    15241 cggccgcgcc gaagcccacc gcgcgtatgg cctccaggct caagcccggc ccggggcaat
    15301 aggtaatgcg gaagcggcct tcgctggagc cgtgaatgag gtgcgcggcg gcgctgcggt
    15361 tgttgcgcag ccgggcgtgg gccttgaccg cggccagggt ccggggcgtt ccgcgatagc
    15421 cgtgctggcg gatcaggcga tcaatctcgg cgtcttcgcc gaaggtctgg aggccgggcg
    15481 ccagcacgat gagttcgccg ccgtcagcca tcgccatgcg cgtccggtaa atcgccttgt
    15541 tccccagcca ggtgcttgtg aattcgcgcg ggtcgaggaa aacgaccact ttcttcagcg
    15601 gcttcgccag gagattcagg ttgaccttct gggagagccg cgcgcccagg gtgaaggttt
    15661 ccatgtcgtc gcccacgtaa agcccgcgca tgatcatgcc gcggcccgcc gggtttggct
    15721 ccatcacggt caggatatag acgatgggca ggtcgcggag gaaagcctga acgccgtagt
    15781 tgaacaggcg gcggacgggg gtttcgatgc gccccatcat gcgctccatg ccgaaagccg
    15841 cgccgaggaa gtgcgatttg ttgatgatgt ccgcgccgcc ggcgccgacc accacattct
    15901 tggtgtagtt ggccatgccc acgacctcat gcggcacaat ctggccgacc gagaggatga
    15961 gatcgtagcc ggcgaacagg agctggttga cctcgatgcg cacggaataa tccacccgcc
    16021 cctccgacca ttcgcggatg aggcggccgg gcacttcgcc cacgcgccgg aggccgtgcc
    16081 gccaatcgtg cacccggaac cgctcccgcg ggatgctccg gccgaacatc aggcgcaact
    16141 gcgcctcggt catcggcgca tgcgtgccca gcgcgggcat gatgtccacg gtggcttgcg
    16201 gggcgagcat cccatagagc aggcgggtga gttcgccggc atccgagttg aggcgggtgt
    16261 gatccggcgg cagcaccagc actttccgca gcggccgccc gatgaccttc agcgcttgcg
    16321 ccagcgccgc gcgtttttcc gcggtgcgga gattatccgc ggttccgccg cgggcaaaat
    16381 aggtagccat aaccgggttt cagagcgggt ttatcattcg gcgggtcaaa ctccgctgaa
    16441 agcgctgaat ccgccatcca ccgcgatgac ctgcccggtc acaaagcccg agccttcgcc
    16501 gctcagcagc cacagcagcg tgccgatcag gtcttccggt ttgccgaaat gcccctgcgg
    16561 ggtgtgggcg gtgatggtgg tgccgcgcgc cgtcagcgag ccgtccggct gggtcagcag
    16621 gccgcggttt tggtccgtga gaaagaagcc cggggcgatg gcattgacgc gcacgccggt
    16681 ccgggagaaa tgcaccgcca gccactgggt gaagttgctc acgcccgcct tggccgcgct
    16741 gtaggcgggg attttggtca gcggccggaa ggcgttcatc gaggagatgt tgatgatgtt
    16801 gccgcgcccc tgcagcacca tgtcccggct gaagacctgc gtgggcagga acgtgcccag
    16861 gaaattcaga ttgaagacaa actggatgcc ctcggccgtc agatcgtaga acgtcaccac
    16921 gccttcctgc ggagccgtga ggtcttcgcg ggagaggaac tcgcgggagg tcgtgccgcg
    16981 ggggtggttg ccgccggcgc cattgatgag aatatccacc gggcccaggg ccgctttgac
    17041 ctgctcgtgc gcctgttcga ggctggcctt gtccaagacg ttggcggcga agccccgggc
    17101 aacgcctcca gcctggcgga tccggtccgc gccctgctgc gccttgtcga gcttgagatc
    17161 gagaatggcc accttcgcgc cgcactgcgc caccgcgtcc gccatcgcgc cgcacaggac
    17221 gccgccgccg ccggtgatga ccgccacctg gtccttgagg tttatttgga acggtatcgt
    17281 cataggaata cgcacatagt tgctcgcttc ctccggcatt tgtcaagccg ggccggctgg
    17341 cgggggaatt tccggctggc caatgccgct ctttccggct aaggtcggag cgcaatgata
    17401 agttccttgc ccaaaccgga agtcatcatc acccacgaga gcgaccttga tgggctggtg
    17461 tccggcgtgc ttttgcagcg gctggcgcgc aagctcttcg ccgcggatgt tcgccttgaa
    17521 gcttacaacc atcacaactg gcggcagcgc gagttgcgcg agaaatccgc gtgggtggcc
    17581 gacctggcgt atgagccccg cttggaccgt ccgaactggg ttattattga ccatcatgcc
    17641 acggccctgg ccccccgaaa tgccgtgttc atccacagcc tgaataaatc agccgcgctg
    17701 ctctgctatg aattgtgccg ccagcagggg ctgggctccc cggaactcga ccggctggtg
    17761 cacctgacca atgtgggaga cctgtttctg gaggacgacc ccgacttcgt tctcgccagt
    17821 gactatgcca gcctggtcaa gcattaccag ttctggaacc tccacgccct ggtcaacggg
    17881 caaatcgagc aactgctcaa taatcccctg ctccgcgtca tggaggtcaa gcgcaacatt
    17941 gaggatccgc tggggtttgc gtgggcccgg gagaatatca atgaaatctc gccccaggtg
    18001 ggttttgtgg acaccatcat tggcaatacc aatctgatcg tgcaccaact cctggagcaa
    18061 ggcgcgacga agtatcccgt gctgctgacg ctgttccgcc gcgccaacgg ggtcatcatc
    18121 gccagcctgc gcagccgcaa tggccaggct gtcaaagttg tcgagaaact ccagggcggc
    18181 ggccacgcca acgccagcgc cgccaccttg ccacgatcgg ttaagacgat tccggatgcc
    18241 atcacctacc tgcgccaggt ccttaacccc caaaaggaaa ctcccgtaaa cagcctggag
    18301 gaggcttttg cggcttttga ccagtcgcaa aaatcggggg catgagaaat ccccgcgcag
    18361 cggcaggggg ctatcgcccg cggggccggg aaaagccgcg catccgcatg gcgtcgcggc
    18421 gggacgggtg agcttttcgt cgttgctccg ggggaaaggc tttcctactt tgccgcctgt
    18481 ttatggcgcc gccgcaatca gcgacaacaa ctccggtagt ccgggcagat ggccgccgga
    18541 cgaaccaact gcgtcctgtc cgctttcaaa atcacatcgc cccgtatgcc gccggttcga
    18601 ccttgatcga atggggcaat acgcgcgtga tttgcggcgt cacggtcgaa gacgccgttc
    18661 cccgctggat gaaggagcag gccgttcccg ggggctggat taccgcggaa tacgcgatgc
    18721 tcccctactc gaccctgaac cgcaaacagc gggacagcac ccggggaaag attgacggcc
    18781 gctcccagga aatccagcgc ctgatcggcc gggcgatgcg gacggccctt gacctggaga
    18841 aactgggcgc ccgcacgttg tgggtggatt gcgatgtgct gcaggccgac ggcggcacgc
    18901 gcacggcggc cattacgggc ggctacgtcg cgctggccct ggctatccgc aaactgatgg
    18961 cggaaggcaa actttcggaa agcccgctgc gacgcgccgt tgccgcggtc agcattggca
    19021 tcgtccagaa acaggccttg ctggacctct gctacgagga agatgtggcg gcggaagtgg
    19081 atctgaacct ggtcatggat tccggcggcg aattcattga attacagggc accggcgagg
    19141 aggcaacgtt cagcgaggcg caactggccg aaatgctcga gttgggcaag gccggggtgc
    19201 gggaactgct ggccgcccaa caggctgccc tggccgtttt gcaagcccgg tgaaatttct
    19261 gcatttgatc tggagcaacc tgatgcgaag gaagcttcgc actgccctga ccctgctctc
    19321 gatcgtggtg gcattcgttc tttttggttt tctgtgcgcc atccggcagg cgctggcggg
    19381 cggagtgacc atggccgggc aaaaccgcct ggtgacgcgc cataaaatct ccattatcca
    19441 gtccctgccc gaatcgtaca agcatcggat ggagcggatt cccggggtgg ccctggccac
    19501 ccaggaatcg tggtttggcg gcgtttacca ggatccgaag aatttctttc cccaaatgtc
    19561 ggtcgtgccc gaggagtatc tggcgatgta tcccgagtat ctcctggcgc cggaacagaa
    19621 gcaggcctgg ctggccaccc gcaccggggc catcgtcggg cggaaaacgg cggagcggtt
    19681 ccaatggaag attggcgatc ggattcccct ccagtcctcg atctggacaa aatccgatgg
    19741 aagccggtta tgggaatttg acctcgtggg gatttatgac gggcgggata aagaaacgga
    19801 taccagcctg ctctttttcc gctatgacta cttcgacgag gcgcgggtgt ggaataaagg
    19861 gcaggtgggc tggtatatgg ttcaggtgac ggatccgtcc caggcggcgg aaatcgcccg
    19921 gcggattgac gaggagttcg agaactccgc ggcggaaacg aagacggagc cggagggcgc
    19981 atttttgcaa gcctgggcca agcagatcgg ggacattacc ctgattacgg cgacgatttt
    20041 gagcgccgtt ttctttacca ttctgctggt gtcgggcaac accatgagcc aggcggtgcg
    20101 ggagcgtacg ggagaactgg gcgcgctgaa agcgatgggt tttaccagcc cgcaggtgct
    20161 ggcgctggtg ctggcggaat cctgcctgct ggcgctcctg ggcggcggcc ttggcctggg
    20221 gctggcgtgg ctgctcgttg cgcggggcga tccaaccggc gggatgctgc ccatgttctt
    20281 tttcccgaaa ggagacttgc tccgcggggt gggattcagc gcatccctgg gcgtgctcgc
    20341 ggggatcttt cccgcctggc gagcgatgcg ccttcgggtt gcggatgcgc tccggaggat
    20401 gtgaccggat atggcgggtc ccatcaactg gctttcccag gttgcctccg tcagcaagtt
    20461 cggcttgctg agcattccgc agcggcgggg ttcggtcgcg gcggcggtgt ttggcatcgc
    20521 cggggtcgtg gcggtgttgg tgggggtgct ctccatgggc gtcggattcc agcgggcgct
    20581 gcaatcttcc ggcgcgccgg atgcggccat cgttctgcgc agcggagccg acagcgagat
    20641 ggttagtggc ttcctgcggg aaagcacgcg cgtgattgcc gacgcgcccg gcgtggcgca
    20701 tacggaggat ggcccgctgg cctccgcgga actttacgtg ctcatcaacc tgctcaagcg
    20761 ctcgacgggc accgacgcca atgtaccgat gcgcggcgtg gagcaggccg cgctgcgggt
    20821 gcgcgaccag ttgcaaatcc tggacgggcg catgttcgag tgggggcgca atgaggtcat
    20881 catcggggtg ggcgccgcca gcgagtttgt ggggctggaa ttggggggta atctgcgcgt
    20941 cgggcagcgc gagtggccga tcgtgggcgt cttctccgcc ggaggcggca cggcggaatc
    21001 ggaaatctgg accgatgcgc gcgtgctgca atccgcctac aagcgcggcg acacctttca
    21061 atccgtccat gtgaagctga gttcgccggc ggcctttcag gaattcaagg atgccctgac
    21121 caccgacccc cgcctcaacg tgaaggtgct ccgccagacc gagtattacg cccaacagtc
    21181 aaccgccgtc acgcgcctga tccgcacgct gggctatctt atcgccttcc tgatggccat
    21241 cggggccgtg tttggcgcgc tcaacacgat gtacagttcg gtatcggcgc gaacgcgcga
    21301 gattgccacg ctgcgcgcac tggggttcgg cagcggagcg gtcgtcctct ccgtaatgat
    21361 cgagtcgctg gtgctggcgc tcattggcgg cggcgtcgga gcggttggcg cttacctcac
    21421 cttcaacggt ctccacacct ccacgatgaa ttggaacagc ttcagccagg tgacctttgc
    21481 cttcgcggtg acgccggaac tgctggggca agggattgta tggtcggtgt tcatcggcct
    21541 gattggcggg ttgtttccgg ccttgcgcgg cgcccgcctg ccgattgccg ccgctctgcg
    21601 cgagttgtaa tcgtccgctt agcgtttcgc cggggcagga gcattggttg cgctggttat
    21661 cccggcgccc gccgctttct gcttttggcg caccgccagt ttttcaaaac gcttcacccc
    21721 caccacggcg gcgatcaagc ccgcaagcag tatgccgatc ccgatcaggc tgtaaacgat
    21781 cgttgcccgc gagatggttg actcttccgc cggtggcgcc aggcgcaatt ccgtctgctg
    21841 cttgcagcac gggcagggcg cccgggtgcc gatcatttcc gccggaaagg tgaaggaacc
    21901 gccgcattcg gcgcagacgc ccggcaaacg cttggttggc ctcataacgc tcgctggaat
    21961 acccgaaaac gtggccgccg tcacgccctt gcgcattttt cgcttggcgc ggatgccgga
    22021 tcccggaatc atcttctgtc aatgacgttt cttcgccgta aatttgaggg ctcgccggag
    22081 tacgcgcgag ttgcgccgtt cgtgattttc gtcgcgctca cggccctgca agggcacctg
    22141 ggtgaggatt cgcgttattg gatttatctc ctcaagacgc tggtgggcgc ctggctgatc
    22201 tgggaaatgc gcccgtttgt cctggagatg cgctggaagt tgagctggga agccgttttg
    22261 gtgggggtgg cgatctttgc tgtctgggtg ggactggatg gctggtatcc ccggctgagc
    22321 gaagtgacgg aaccgggctg gaatccgcat ttgcggtatg gcgaaggctc cgccctggcg
    22381 tggctctacg tagtgacccg catcgtcggt tcatccctgg tcgtgccgcc gattgaagag
    22441 gtcttttatc gctccttcct ctatcgcgtg cttgtgaagc gggactttcg cgccatgccg
    22501 ctcagccagt tccacgggct ttcttttgtc gtcacctcag ccatctttgg ccttttgcac
    22561 ccggatcgct ggctggccgg catcttatgc ggcctggcct accagtggct ggtcattcgc
    22621 aagcaacggc tgggcgatgc gatgacggca catgccatca ccaattttct gctcggcttg
    22681 tgggtggctt ggaagggagc ctggattttc tggtgagcct gggcaagggc aacaactttc
    22741 taataccggg ccagaggtcc gctcttgaat cgcgaaagca acggcgcggc ttccaacggc
    22801 caggctaagc cgcccgcgcg aaaatcggcg tttaatttat catcccgtgc tgccgtgcct
    22861 gatattcgag gatgagcatt tgctggtggt caacaaaccc gccggtctga atacacatgc
    22921 ccccagcccc tacgccgggg atgggctcta tgactggctg cgccaccgcg agccccggtg
    22981 ggcggccctg gccattattc accggctcga taaggaaact tccggcgtga tggtctttgc
    23041 caagacgccg caggccaacc gctccctcac ggcgcaattc acagcgcgcg tggtgcgaaa
    23101 gaaatatctg ctgctgaccg atcgcccggc ccggcaaaag gagtttgtgg tcaagagcac
    23161 gctggttcga cggggcgacc gctacctgag ccaacccctg acacgcggcg gcgggattgc
    23221 ggaaacccgg ttccggtcag tcagaatgcc gcatgtcccg ggcaaaacag ccgatcactg
    23281 gcactggttc gaagccgaac cgctaaccgg gcgcacccac cagatacggg tccacgcagc
    23341 cgcgaacggt attcccatac tgggggacac gctatacggc ggcacgcctg cgccccgggt
    23401 ttgcttacag gctgtggaat tgcaactaaa acatcccgct tccggcgaag taatgcagtt
    23461 ctccgttccc atagacctcc cgcctacagg catccagagt tacgcggcgg cgcagacgaa
    23521 gggcggtttg ctgtggacta ctgcttcctg gccgtctctg gctttgcgcc aggcgacgat
    23581 tgaaggcgag gaaacccaag cgtatcggct tgtccatggc gcgagcgacg ggtgccccgg
    23641 ctggtatctt gaccgcgtgg gagatttcct gctctcgcaa agcgagtcgc cgctcaccgc
    23701 agcgcaacag aacgaactgg agcatttcgc cgcaaccctc gcccggcagg gcgcctacca
    23761 caagctcctg gcgcgtcaac cagggcaggt tcaacgcctg gcggcttcac cccggcttgt
    23821 tttcggaaaa cccgcgccgg aacgtttcgc ggttcgcgaa aacggcgtag cgtatgaact
    23881 cagctttagc gaaggctatt ccatggggct ttttctcgac caacgcgaca accgtcgccg
    23941 tttgctcagt cgccatgttg ccgcgggctt cccgctttgc cttccgccgc ccgcctcccg
    24001 acaatgggaa gcgctcaaca cctttgccta tacttgcggg ttttccgttt gcgcggcacg
    24061 cgcgggagcg cgtgtaacga gcattgacct ctcgaagcgc tacctggaat ggggccggcg
    24121 caactttcgc ctgaaccagc ttgacccgga acaacacaac tttatacacg gcgacacctt
    24181 ggactggctg cggcggctgg aaaagaagaa gcggcctttc gatgtgattc tgctcgaccc
    24241 gcccaccttt tcccagtcga aaacgtccgg aatatttcgc gcggaaaagg actatgaaaa
    24301 actcataacc gccgcgttac ccctgctccc ggccggagga gttctgtttg cctcgacgaa
    24361 tgccgcggct tggccgcccg aagaatttat cgccgctatc gagcgagcta tccgccgggc
    24421 gcgccggaaa attatacagc gtcattatgt cccacaacca cttgatttcc ctgtttcacg
    24481 cgccgagccg gcttatctga agaccgtgtg gctgcgggtg gaatagcatg ctcatccacg
    24541 catccgcgca agtcatgcac gccacgctcc ctgcctcgcc gcaccctcaa tcgctgccct
    24601 ctta
//
LOCUS       DAOPQG010000053        24580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2572_length_24580_cov_15.422671, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000053 DAOPQG010000000
VERSION     DAOPQG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 24580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24580
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2572_length_24580_cov_15.422671"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1703
                     /gene="cimA"
                     /locus_tag="PKI07_07745"
     CDS             138..1703
                     /gene="cimA"
                     /locus_tag="PKI07_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOC50984.1"
                     /translation="MIMKPEVEIYDTTLRDGSQGEGINFSARDKVRIAEKLDAFGIHY
                     IEGGWPGSNPKDMEFFKQAARRPWKNARIAAFSMTRRKGMAVENDALMRQILEAETPV
                     VTIVGKSWLLHVTEVLRAKPDENLAMIADTVRYLKDQGRTVIYDAEHAFDGYKEEPEY
                     ALATWAAAARAGAECIVLCDTNGGSLPRDIARIVGVAKEKLNTRLGIHTHNDCGLGVA
                     NGLAAVEAGATQIQGTINGYGERTGNCNLTSIIPLLALKMNLPILPAGSLARLKELSE
                     FVNEIANIRHDPRQPWVGQTAFAHKGGMHVHAIDRVARSYEHINPAAVGNTRRVLVSD
                     MSGRTNILLKAKELGFKLAADAPETRTITDQIKRRENEGYEYEAAEASLALLIGRALK
                     ETKLPFTVNAYHVSMRRDGASSICEATVKVRVNNENGHTVAEGDGPVNALDKALRTAL
                     VKFYPQLKDITLTDYKVRILETSTGTGARTRVLIHTSDGRQEWGTVGVSENIIEASLQ
                     ALVDSLEYALTRK"
     gene            1713..4415
                     /locus_tag="PKI07_07750"
     CDS             1713..4415
                     /locus_tag="PKI07_07750"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOC50985.1"
                     /translation="MVELPKAYEPQAVEARWYDFWLKHQCFAANPASAKPAYAIVIPP
                     PNVTGMLHMGHVLNNTIQDILARKARMDGREVLWLPGTDHAGIATQAVVEKTLSQQGR
                     MKHRDDLGREKFLEHVWAWKEKHGGIIIQQLKKLGCSCDWARERFTMDPDYTRCVQKA
                     FVELYRKGLIYRGRRMVNWDPKARTALSDEEVEMLEQKGHLWHLQYPLLDEHDRPRAG
                     EFLVVATTRPETMLGDEAVAVHPADPRYQKILGRKCLLPLQNKPIPIIADELVDPKFG
                     TGCVKVTPAHDPADYEMGLRHQLPFTVVIGPDGRMTAAAGEKFAGLDRAKARQAVVQE
                     LTGLGLLLKIEDYVHNVGYSQRSQVPIEPYLSEQWFLKYPSVEPSIQAVAEGRIAFHP
                     DRWGKTYSHWMHNLRDWCISRQLWWGHRVPVWYSRAEGQAGKIWCDLTPPPDPENWEQ
                     DPDVLDTWFSSWLWPFATMGWPEATETLKKFYPTTDLVTGPDIIFFWVARMIMAGFEF
                     MGEVPFRNVYFTGIIRDKQGRKMSKSLGNSPDPLDLIGKYGADALRFGTMRSAPLGQD
                     VLFDEKDVELGRNFCNKLWNACRFRQMQGGEVQGEIQPGLLTGDDQWILLKLDQAIRE
                     ITAAFAEYRFNEATQTLYRFFWSEYCDWYIEASKAILSPQNTPARPDADARRANTLAV
                     IDFVLSHTLRLFHPFLPFITEELWQGMGYHEDMPAAQGGQTIMSAPWPQPLDGDFRGH
                     YGLNAAVLERVNARNDLVSQGRNLRREGNIPANKKVRFVFRPAHPVQPHDAEVLKLLL
                     NAEALEIDPRHQPPKGTPSVQSELGCLYLPLEGLIDVAAEKGRLAKELEKAESEIAKT
                     RQKLDNPAFTAKAPPHVLQTHQQRLVEWQTRSERLRQALAALED"
     gene            complement(4522..5793)
                     /gene="eno"
                     /locus_tag="PKI07_07755"
     CDS             complement(4522..5793)
                     /gene="eno"
                     /locus_tag="PKI07_07755"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOC50986.1"
                     /translation="MSRIQDIQAREILDSRGNPTVEVDVTLASGASGRAAVPSGASTG
                     EHEALELRDGDRKRYLGKGVTKAVKNVREKILPRLAGMDALDQLAVDHAMLKLDGTET
                     KAKLGANAILAVSLANAKAAAALLGQPLFKYLGGPNAKVLPVPMANVINGGAHSDAPI
                     DFQEFMIMPKGLPTFSEGLRAITEIFHALKAVLKKKGLSTAVGDEGGFAPKLASTEAA
                     IEAILQATQEAGYKAGKQIFLALDVAASEFYNGHGTYTFKKSTGQTVSGDELIAFYVK
                     LCRKYPIVSIEDGCAESDWTNWKKLTDKLGGQVQLVGDDLFVTNVKFLRRGIDTGTAN
                     SILVKVNQIGSLTETLDAVELAQDNNYTAVISHRSGETEDATIADIAVATNAGQIKTG
                     SLSRTDRVAKYNQLLRIEQLLGKNAVYGGKF"
     gene            6202..6903
                     /locus_tag="PKI07_07760"
     CDS             6202..6903
                     /locus_tag="PKI07_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC50987.1"
                     /translation="MALPAPLLQLANVTRRYDTPGRSESLTVLNEVSLEVAVGESLAI
                     IGPSGSGKSTLLQIAGALDRPSSGSVLLNGRDLGALDEEELAAVRNRQIGFVFQAHYL
                     LPQCTVLENVLVPTLAFRRSAARDGAAQRARHLLRRVGLGERFAHRPGELSGGERQRV
                     AVVRALINQPQLLLADEPTGSLDDAAARELGQLLLELNREAGVALMVVTHALSLARRM
                     GRMLELKGGRLAAVS"
     gene            6913..10437
                     /locus_tag="PKI07_07765"
     CDS             6913..10437
                     /locus_tag="PKI07_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC50988.1"
                     /translation="MTFGTLIHRSLRFHARAHLGVALGAAVGSAALIGALVVGDSVRE
                     SLAGMARRRLGKIHFALATQDRLFPANLRERLAGAKPPDSADPSAAAANRAVPALLSA
                     ALTLPGIATRPDGAARVNHVQVLGVDPQSWPRLAHWGSLSPDARRRGPAREPPNRGGV
                     VMPPPEEEEELLARWQRGGMAFINPALARKLAVRAGDELIVRMRKPSALGLDAALSPR
                     NEDAVALRLKVGAILPSERLGDFALTAQPGPAANLFLPLDFLSAQLGVPGQANLLAAG
                     PEWDLPRLGRELARAWQPEDAGLTVRVVAPPPDRTGGERVPPFVEICSARIFLEPSIV
                     AAALTPRADWRSDGTGESARGPLATNGMRVLTYLANLMRAGNRATPYSMVTAAEAPLV
                     PPDMRDDEVLINEWLADDLQVKPGDAVALSYYVVDSGSRLVERTNSFRVRQVVPLRGR
                     YADRTLMPEFPGVAQAERTRDWDAGFPLVYPIQARDEAYWKTYRGTPKAFVTLAAGQA
                     MWASRFGTLTAIRYPVPDDTPPDTVREAVKRQLRTGLRPDEAGLQFEAVRAAALKAAA
                     QGQDFGLLFLGFSWFVVGAALLLMALLFQFELEQRSAEVGILLALGLTPRQVRRLLLF
                     EGAALALAGGAIGALGGLGYAWAMIQALTTVWRGAIGSAELHFQATAASLLTGFCAST
                     VVATLTIWLTLRRQARQPVRALLTGEIPSPVPRHRNRGPWAAAGAGAAAAGLVSWALL
                     SHRQAEPGVFFGAGVLVLLAGLAAASDWLGRRAGAADAARLTLPGLGARGCARRRNRS
                     LACVALLACGSFVLLAIGVFRLEAQPDAVRRDSGTGGFALIGESTLPVVADLNTRTGR
                     DFYGLDDAPLAGVNVVPLRVREGDAANCLNLNRAQRPRLLGVKPERLAGRFAFTPAAG
                     YDRRWGWALLSANPRRPGALPDAVPAIGDANSIQWALGKKIGDTLDYTDELGRAFKVR
                     LVGAVANSILQGSLLIDEAEFTRRFPGESGYRMFLVDAPADAVPRVSAVLTRALQDAG
                     LELTPAARRLNEFNTVQNTYLGTFQALGGLGLLLGSAGLGVVVLRNVLERRGELGLLG
                     AVGFRRRQLQWLVMAEHGVLLGLGLGLGIAAAMVAVLPALLTPGPPRSYGWLALTLAA
                     VLLNGLLWTWLAAAAALRGNLLAALRNE"
     gene            complement(10443..11915)
                     /gene="gatA"
                     /locus_tag="PKI07_07770"
     CDS             complement(10443..11915)
                     /gene="gatA"
                     /locus_tag="PKI07_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOC50989.1"
                     /translation="MLNQLTISELTAKLARREVSARAATQACLDRIQSVDGLIRAFIS
                     YDAADALAQAGEADRALAAGQTHAQRPLLGVPLAVKDAIAVKGQPLTCGSRMLEGYIS
                     PYDATVIEKLKAAGAVIFGRLNLDEFAMGSSTENSAFQVTRNPWDPSRVPGGSSGGSA
                     AAVAADECIVALGSDTGGSIRQPASFCGCVGLKPSYGRVSRYGLAAYASSLDQIGCLA
                     KDTRDAATVLGVISGHDPRDSTSAPLPVGNYAAGLTGDIKGLKLGLVKEYRVGGLEPA
                     VRQALEAAERQFTRLGAELVEVSLPHTDYAIAAYYIIATAEASANLARFDGIRYGLRV
                     SGSDPTELYCRTRGAGFGPEVKRRIILGTYVLSSGYYDAYYLRAQKVRTLVRQDFQAA
                     FDKVDAILAPTTPTTAFPAGEKTADPLQMYLMDIFTISCNLAGLCGLALPGGFTEGNP
                     RRPVGLQLLGKPFGEETLLRLGHAYEQSTEWHQQKPPLAG"
     gene            complement(12058..13242)
                     /locus_tag="PKI07_07775"
     CDS             complement(12058..13242)
                     /locus_tag="PKI07_07775"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOC50990.1"
                     /translation="MRLSDGGFPGAAAARRRRWLTGLTVSLWLAGGVSVAADSSPTNG
                     VVRWLDLPHPQLEICGLPWFKENAPDLWRLPKRAQTRVPKGVWNRAVSPGGGRIRLSC
                     NSSCLGLRLAAVQPGGKRCCFDAFVNGQYAGSVSPQGTQRVDRVIFANQGRAWKDITL
                     YLPHRNEVRVFAVGLDADAELRPPPPFAQPRPLVCYGSSVLQGTGALHPAQTYPAALA
                     RRLNLDFVNLGFGGAGKAEPEVVALVNQLDAGAYLFDLGKSYGAPEPERYNRMLDAIR
                     AAHPTAPIFCVTPIYSTREAAQPDYRQRSEALRALMRRAAEERRQAGDPRLFVVEGLE
                     LFGEPDKALLADAAHPGDEGNELMARRLAARLEEVLGGRGQPLPGGAAARTNAAPPGA
                     RP"
     gene            13325..14224
                     /locus_tag="PKI07_07780"
     CDS             13325..14224
                     /locus_tag="PKI07_07780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC50991.1"
                     /translation="MRYRILGSTGLRVSVIGLGTWQFGGEWGRAFSQADADAILDQAG
                     ALGINLLDTAECYGDHLSESLVGDYLSRHDRSRWIVATKFGHRFQRFMTRAEDFSPAG
                     VRQQLEASLRALRLEAIDLYQFHSGSDSQLLNDDLWAMLAEQKQAGKIRHLGISIPGK
                     GSEPQAREARRLGAETLQVIYNRLDRRPEQLYFPHAQRDHLGILARVPLASGLLSGKY
                     RADAAFPAQDWRSTLDADKLRRDLAEVERIRQSELPAGMAMAQWALAWCLQHPAVSTV
                     IPGCMNPAQVAANAAAAELELPR"
     gene            complement(14240..16546)
                     /locus_tag="PKI07_07785"
     CDS             complement(14240..16546)
                     /locus_tag="PKI07_07785"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOC50992.1"
                     /translation="MLAAAGALGASCVMAQLALMRELLGVFAGNEMVLGVTLGWWLLL
                     MGIGARLGRRAERLRDPLAALARVLILAAVLPPLQVLAVRVLRNVVFPRGAAVGMAET
                     AGAALALLLPYCLVAGYGLTLACSIRSRVEGARGIGRVYVADSLGSIVGGVGFSFVLA
                     RFLDHIALLAVPAGLNLALAWLVAWPAPGAGRAARGWLLAAVPVLGVGLAGVTLLADP
                     DGWSTARQYPRQEVVAHANSPYGRLVVTRSAGQYDFLENGIPVASTRDDQRVEETVHY
                     ALAQRPAARRVLLVSGGISGTAREILKYGVGAVDYVELDPLVLALGRKYLPQNLDDPR
                     IRVSNTDGRLFTRQAAEKYEVAIIDVPAPASAQLNRFYTAEYFAEVKGALAPGGVVCF
                     ALGQYENYVSPELARLLSSARRTVEQSFRNVLVIPGQRVFFLASDGPLSADIAARLEP
                     RGIPTRLVNRHYLSAMLTPDRMQALADATVQPAAVNRDFSPVLYYYHLRHWLRQFDAR
                     FGLVEAALLLGLGYYVARLRGSALVLFASGFAASALELVLLLAFQVLCGSVYHQLGII
                     VTVFMAGLALGAALMNRLAVSPHLHPPGVLTSQPAPRLLAIRMAGASRRWGAAADAGM
                     AMLAGCPGRRFIRWLANGRGNGLSWLAFLLAAYAAVLPLLLPLLNRMGGPAGSRVMVK
                     AGIALLTLVLALLVGMQFPLANRREYDGTVRGAARLYAADFIGACLGALLACALLIPL
                     LGVGGACLVTALLNLAAGWAGRRPAAGV"
     gene            complement(16560..18203)
                     /gene="amrB"
                     /locus_tag="PKI07_07790"
     CDS             complement(16560..18203)
                     /gene="amrB"
                     /locus_tag="PKI07_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOC50993.1"
                     /translation="MRLPFGGKSVCHDGMAEHVETRAANRGGRGKCVAAVLALLVIGL
                     GCGARPEAPEEKPSRRSVPSKSSAREARRVREPAVAGLFYPGEAKKLSGMIDALLASA
                     PDHSIPRLKGLVCPHAGYEFSGATAAIAYKLLAGRDARTVVVMGASHYALLQGVSLPD
                     ADAYRTPLGLAPISERARALAGVAPFAREPRCPVRRPDWWRQASKAAPEAGQDTPETW
                     EHSVEVQVPFLQKVLPDFQLLPMILGEVNPEQVARALAEQWDDKTIVVASTDLSHFHA
                     YETARGLDRRCLRAIEALDLEAMSAQEACGKLPVLALLHLARQKGWKAQVLDYRNSGD
                     VSGDKNRVVGYAAVAFYAPGPEQVAASERKFLLELARRTLRSVTASGGLPEVADADVP
                     PKLREKRACFVTLTKGGALRGCIGHLTALEPLHRAVAENARNAALRDGRFPPVQAGEL
                     DEIRIEISVLTEPQPLAFTSPDDLLAKLRPHEDGVLLRIGARTATFLPQVWAQLPDKV
                     QFLEQLSRKAGGDASAWRGKDVTLSIYHVECFAEEPPRS"
     gene            complement(18166..18723)
                     /locus_tag="PKI07_07795"
     CDS             complement(18166..18723)
                     /locus_tag="PKI07_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50994.1"
                     /translation="MDRVNGWAEVLQRLGDVEGNIPARLFIHRRCGRLVETLPSLQHD
                     PSRPEDVLKVDADGEGVGRDDAADALGIWWRRKRLEAGNYALSGLGWLRGRYSQGVAL
                     GYRMAPRWGAGAGERGWCSWGVAPGYRMAPRWGGGWAGRGERAVAGVAEDDVVEDFGF
                     EELANADEVAGENYALAFWWQKRMP"
     gene            18836..20176
                     /locus_tag="PKI07_07800"
     CDS             18836..20176
                     /locus_tag="PKI07_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOC50995.1"
                     /translation="MTRISSPLSRRRFMERSALATSLFGAAGLASPGHSDAADAAAAA
                     APAGAVPRVNARQHGAKGDGQSDDTRALQSALEAARKDGPICYVPPGLYRLAGALTVP
                     PGVTLCGASGGVPHSEHPIGTVLLAVGGRGQADGEPLITLKPNAVARNLIIHYPEQTL
                     AEVAPYPWSIRADGELCQIRDITLTNPYQALDLGSRWNELHLVRNVFACPLKIGVYID
                     QCTDIGRIENVHFNPNFWTRMALKPGFPGGDIKAYLEQNLVGFKIGKTDWEFISNSFV
                     IFPRIGFHFDDFGHGPGNAVITQSGSDIGPVAVQVDRSQPHAGVQFANAQFMSTLVVG
                     PNNQGPVKLANCGFWGIPETREQVRHSGPGTLALTACHFNGWDQPGKGAPCIRAAGGR
                     LMVNGCEFMDGGKRAIVLEKGLVAATIFGCAFRGAAAVDDQSGADVQIGLNTSR"
     gene            complement(20295..22487)
                     /locus_tag="PKI07_07805"
     CDS             complement(20295..22487)
                     /locus_tag="PKI07_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC50996.1"
                     /translation="MSNYPFISGWTHNVTRRAPPLSFAIALLAATLLPVTAFAAAPQA
                     PAQGTHVPVIVNVYSNANVSTQYIADAIAEANKILGQANIRLVPVKINDPVTAANNGD
                     DGDGVFTRAERDAIRTFGGKEVAGLPGSTGMKISFGQQVNATNASNIGVAVHRDPTII
                     VQKYGSTTDTAQTIAHEAAHVFTLGAYHKIATGIDADEDGHSSTNNPGPAGEENLMAP
                     KHTPGATNLTKLQIEEMNKEVTTFGFSARQFKENCPAVVGLVQSGAARDDHGDAISSG
                     GHGADVKDIHDLQRVWVGSEQGVPTLTVSLGVGGVIPSEGTLAARYALGLDTDNNPLT
                     GVSYAGRAGIDRIVRVEVSRPTEPGPLLVQGFVEDPMMPPPGMLLPGPIELLTEPEFN
                     DREGPTVPIGTTIAFQVPKSMLALTAPQVPIVAAAGIPVEFFDTVELTFDQMLYAHRP
                     DLRLYGSGVPRAGVPYPFTLTRMSPNQPVQLYLDDTLVYNGTLNAAGSCSGCFLPPLG
                     LENGSLHFLTALDQTESFAGNITSPKPELSVSARQVGMEVELCFDTSLGVDYVVERAE
                     EVLPPGWEIWDIPTGTGEPVVMKDTLEPGGRYYRVGMQSVNMDITQIAMPNPVRVGQE
                     LTYTLEVKNLGGPPARKVQIFDPLPASVEYISAEVLPPEEGNCVYSDGMLTCELLNPL
                     LPGPGVSVVIRVRPQQPGSLENTAIVANPAPDPVAGNDSATVVTTVLP"
     gene            complement(23049..23978)
                     /locus_tag="PKI07_07810"
     CDS             complement(23049..23978)
                     /locus_tag="PKI07_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC50997.1"
                     /translation="MATNTANPINRRTFLGGVAGMGMAGMSVLKLPALLAAEAAPARK
                     PNWQIGCYTRPFDQFDYPVALDAIAEAGFRYVGIMTSNIAGWVMIKSSTPVEEVRNMH
                     RAARKRGLEVLSIYGEFPVSSSLEENCRGLKQLIDDAAICECPHLMLGGIGDERYQIY
                     YQTVAECCDHAAAKGVNLSIKPHGGRNATGPQCRKAIELVGRKNFGLWYDPGNIFFYS
                     DGQLDPVADAATVDGLVMGMSVKDYRPPKDVLVNIGEGRVDFPKVFDRLKQGGFTGGP
                     LLVECLARGALADVKAAARKARLFLEQLTGRKS"
BASE COUNT         4226 a         8616 c         7773 g         3965 t
ORIGIN      
        1 gcggggccct tggttatcgg cgcgcaagtt tcgctagatg cgggggctgg gataagacct
       61 gcccggcgca agggcgtctc tttcgccttt gcatccgtcc cggcattgcg taattattcc
      121 agcttgctat tgcgggcatg attatgaaac ccgaagttga aatctacgat acgaccctgc
      181 gcgacggcag ccagggcgaa ggcattaatt tttccgcgcg ggacaaggtg cggattgcgg
      241 agaagctcga tgccttcggc attcattaca tcgagggggg atggccgggg tccaatccca
      301 aggacatgga gttcttcaag caggcggccc ggcggccgtg gaagaatgcc cggatcgccg
      361 cgttcagcat gacgcgccgc aaggggatgg ccgtggagaa cgatgcgttg atgcggcaga
      421 tcctcgaggc ggagacgccg gtggtgacga ttgtcggcaa gagctggctg ctgcatgtga
      481 ccgaggtttt gcgggccaaa ccggacgaga acctggcgat gatcgccgac acggttcgct
      541 acttgaagga ccaggggcgg acggtgattt acgacgccga gcacgccttc gacggctata
      601 aagaggagcc ggagtacgcg ctggccacgt gggcggcggc ggcccgggcc ggcgccgagt
      661 gcatcgtgct gtgcgatacc aacggcggct cgctgccccg ggacatcgcg cgcattgtgg
      721 gcgtcgccaa agagaagctt aacacccgcc tgggcatcca tacgcataat gattgcggcc
      781 tcggggttgc caacgggctg gccgccgtcg aggccggggc cacgcagatc cagggcacca
      841 tcaacggcta cggcgagcgc accggcaatt gcaatcttac gagcatcatc ccgctgctgg
      901 cgctgaagat gaacctgccg atcctgcccg ccggctccct ggcccggctc aaggagctgt
      961 ccgagtttgt gaacgaaatt gccaatatcc gccacgatcc gcggcagccg tgggtcgggc
     1021 agacggcgtt tgcgcacaag ggggggatgc acgtccacgc cattgaccgg gtggcgcgca
     1081 gctacgagca catcaacccc gcggcggtcg ggaatacccg gcgcgtcctg gtcagcgaca
     1141 tgtcggggcg gactaacatt ctgctgaagg ccaaagagct cgggttcaag ctggcggcgg
     1201 atgcgccgga gacgcgcacg attacagacc aaatcaagcg ccgggagaac gaaggttatg
     1261 aatacgaggc cgccgaagcc tcgctggcgc tgctgattgg ccgggcgctc aaagagacga
     1321 aattgccctt cacggtgaac gcctaccatg tctcgatgcg ccgggacggc gccagttcga
     1381 tctgcgaggc caccgtcaag gtgcgggtga acaatgagaa cggccatacg gtcgccgagg
     1441 gggacggccc ggtgaacgcg ctggacaaag ccttgcgcac cgccctggtg aagttctacc
     1501 cgcaactcaa ggacatcacg ctgacggact acaaggtgcg cattctggag acttccaccg
     1561 gcaccggggc gcggacgcgg gtgttgatcc acaccagcga cggccggcag gaatggggga
     1621 cggtgggggt cagcgagaat atcatcgaag ccagcctgca ggcgctggtg gacagcctgg
     1681 aatacgcgct gacgcggaag tgagccgccg ccatggttga actcccgaaa gcctacgaac
     1741 cccaggccgt cgaggcgcgg tggtatgatt tctggctgaa gcaccagtgc tttgccgcca
     1801 atcccgcctc cgccaaaccc gcctacgcca ttgtcatccc gccgccgaac gtgacgggca
     1861 tgctccacat ggggcacgtg ctcaacaata ccatccagga catcctcgcc cgcaaggccc
     1921 gcatggacgg ccgggaagtt ctgtggctgc ccggcaccga ccacgccggc atcgccacgc
     1981 aggcggtggt ggaaaaaacc ctcagccagc aggggcggat gaagcaccgc gacgacctgg
     2041 gccgcgagaa gtttctggag catgtctggg cctggaagga aaaacacggc ggcatcatca
     2101 ttcagcagct taaaaagctg ggctgctcgt gcgattgggc gcgcgaacgc ttcaccatgg
     2161 acccggacta tacccggtgt gttcagaagg ccttcgtcga gctttaccgg aaggggctga
     2221 tctaccgcgg ccggcgcatg gtgaactggg atcccaaagc gcgcacggcc ctctcggatg
     2281 aagaagttga gatgcttgag caaaaggggc acctgtggca cctgcagtac ccgttgctcg
     2341 atgagcatga tcggccccgg gcgggcgagt tcctcgtcgt cgccacgacc cgcccggaga
     2401 cgatgctggg ggacgaagcc gtggcggtgc acccggccga cccgcgttac cagaaaatcc
     2461 tcgggcgcaa atgcctgctg cccttgcaga acaagcccat ccccatcata gccgacgagc
     2521 tggtggaccc gaaattcggc accggctgcg tgaaggtgac gcccgcccac gatccggcgg
     2581 actacgagat gggcctgcgc caccagttgc cgttcaccgt cgtcatcggc cccgacgggc
     2641 gcatgaccgc ggcggcgggc gaaaagtttg cggggctgga ccgcgcgaaa gcccggcagg
     2701 ccgtggtcca ggagctgacc gggctggggc tgctgctcaa gattgaggat tacgtccaca
     2761 acgtcggcta cagccagcgc agccaggtcc ccatcgagcc ttatctgagc gagcaatggt
     2821 tcctgaagta tccgagtgtg gagccgtcaa tccaggcagt cgccgaaggg cgcatcgcct
     2881 tccacccgga ccggtggggc aagacctact cgcattggat gcataacctg cgggactggt
     2941 gcatcagccg ccagctctgg tgggggcatc gcgtgccggt gtggtattcc cgggcggaag
     3001 ggcaagcggg caaaatctgg tgcgacctca cgccgccgcc cgacccggaa aactgggagc
     3061 aggaccccga tgtcctcgat acctggttca gctcgtggct ctggccgttt gcgaccatgg
     3121 gctggccgga agcaacagag acgctgaaga aattctatcc caccaccgac ctcgtcaccg
     3181 ggccggacat catcttcttc tgggtcgcgc ggatgatcat ggcgggcttc gagttcatgg
     3241 gggaggtgcc tttccggaat gtgtatttca ccggcatcat ccgcgacaag cagggccgca
     3301 agatgagcaa gagtttgggc aactcgcccg acccgctcga cctgatcggc aaatacggcg
     3361 ccgacgccct gcgcttcggc accatgcgca gcgcgccgct ggggcaggat gtgttgttcg
     3421 atgaaaagga cgtcgaactg gggcgcaact tctgcaacaa gctctggaat gcctgccgct
     3481 tccgccagat gcaaggcggc gaggtgcaag gcgaaatcca gcccggcctc ctgaccggcg
     3541 acgaccaatg gattttgctc aaactggacc aggccatccg ggaaatcacc gccgccttcg
     3601 ccgagtaccg cttcaacgaa gccacgcaga ccctgtaccg cttcttctgg agcgagtatt
     3661 gcgactggta tatcgaggcc agcaaagcca tcctctcccc gcaaaacacc cccgcccgcc
     3721 cggacgcgga tgcccgccgc gccaataccc tggcggtcat tgattttgtc ctctcccaca
     3781 ccctgcgttt gttccatccc ttcctgccgt tcatcacgga ggaattatgg cagggcatgg
     3841 gctaccacga agacatgccc gcggcgcagg gcgggcaaac catcatgtcg gcgccgtggc
     3901 cgcaaccgct ggacggcgat ttccgcgggc actatgggct caacgccgcc gtcctggagc
     3961 gcgtcaatgc ccgcaatgac ctcgtcagcc agggccgcaa cctgcgccgc gaaggcaaca
     4021 tcccggccaa caagaaggtc cggtttgttt tccggccggc gcacccggtc cagccgcacg
     4081 acgccgaagt cctgaagctg ctgctcaatg ccgaggcgct cgaaattgac ccgcggcatc
     4141 agcccccaaa gggcacgccc agcgtccagt cggaactggg ctgcctgtat cttccgctgg
     4201 aagggctgat tgatgtggcg gcggagaagg ggcgcctggc caaagaattg gaaaaggccg
     4261 aaagcgagat cgccaagacc cgccagaaac tggacaaccc ggctttcacc gccaaggcgc
     4321 cgccgcacgt gctgcaaacg caccagcagc ggctggtgga atggcagacc cggagcgagc
     4381 gtctccgcca ggccctcgcg gccctggagg attgagcagc ccggctggcc gccctcccgg
     4441 acgagcgccg gcggacgaaa aaaacggccg cgcccggatg agcgcggccg tgggtactgt
     4501 ggatcctgag cggcccggag actagaactt gccgccgtaa accgcgttct tgcccagcaa
     4561 ctgctcgatg cgcagcaact ggttgtattt cgcgacgcgg tcggtgcggc tcagggagcc
     4621 ggtcttgatc tggccggcgt tggtggccac cgcgatgtcg gcgatggtgg cgtcttccgt
     4681 ttccccggag cgatggctga tgaccgcggt atagttgttg tcctgggcca gctccacggc
     4741 gtccagcgtt tcggtcagcg agccaatctg gttgaccttc accaggatcg agttggcggt
     4801 gccggtatca atgccccggc gcaggaactt cacgttggtg acgaacaggt cgtccccgac
     4861 cagttgcacc tggccgccca gcttgtcggt cagcttcttc cagttggtcc aatcgctctc
     4921 ggcgcagccg tcctcgatgc tcacgatggg atacttgcgg cagagcttta cgtagaacgc
     4981 gatcagctcg tcgcccgaga cggtctggcc ggtgctcttc ttgaacgtgt aggtgccgtg
     5041 gccattgtaa aactcggagg cggccacatc cagcgccagg aagatttgtt tccccgcctt
     5101 gtagccggcc tcttgggtgg cctgcaaaat cgcctcgatg gccgcctccg tgctggccag
     5161 cttcggggca aaaccgccct cgtcgcccac cgcggtcgaa agccccttct tcttcaacac
     5221 cgccttgagg gcgtgaaaga tttccgtgat ggcgcgcagc ccctcggaga aggtgggcaa
     5281 gcccttgggc atgatcataa actcctgaaa atcaatcggg gcatccgagt gcgcgccccc
     5341 gttgatgacg ttggccatcg gcaccggcag gaccttggcg ttggggccgc cgaggtattt
     5401 gaacagcggc tggcccaaaa gcgcggcggc ggctttggcg ttggccagcg aaacggccag
     5461 gatggcattg gcgcccagct tggccttggt ttccgtgcca tcgagcttga gcatggcgtg
     5521 gtccaccgcc agttggtcca gcgcgtccat ccccgcaagc cgcggaagaa tcttctcccg
     5581 cacattcttc accgccttgg tgactccttt gcccaaatag cgcttccggt cgccatcgcg
     5641 caactccagc gcttcgtgct cgccggtgct ggcgcccgag ggcaccgccg cccgcccgct
     5701 cgcgccgctg gccagggtca catcaacttc cacggtgggg tttccgcgtg aatccagtat
     5761 ctcacgcgcc tgtatgtctt gaattctact cataaatatg cgtcgtcggt tcgaaactcg
     5821 gaaaaccatg aaggccgtcc gggcccccgc ggctcgcgat gcgcccacgg ttctttgtga
     5881 accgtgggtt tccatgctca ccttgttcca agtgagggcc ttatactagg aaaacctcct
     5941 tgcgagtcaa ggaatggtaa tctttcgccc aaaccgcgcg ccgcgagccc ggatgggcgg
     6001 atatgcttgc ggccgccaat tcccagcgcc cggaaagccg ggttcacatc aacccccgct
     6061 ttccctgccg gatggaagcc ctcccgcccg cctggccata ctgcccggcg ggaacaagga
     6121 tatccgtgcc ccggccggcg gttttagtgg gggttgccgt ttttcctgtc cttttttagc
     6181 aatcttgcgc cagattaggg catggccttg ccagcaccac tcttgcaact ggctaacgtg
     6241 acccggcgct acgacacgcc cggccggtca gaatcgttaa ctgtcctgaa cgaggtttca
     6301 ctggaggtcg ccgtcggcga atcgctggcc atcatcggcc cgtcgggatc cggcaagagc
     6361 acgctcttgc aaatcgcggg cgcgttggat cggccaagca gcggctccgt tctgctcaac
     6421 gggcgggatt taggcgccct cgacgaggag gaactggccg cggtgcggaa ccggcaaatc
     6481 ggcttcgttt ttcaggcgca ctaccttttg ccccaatgca ccgttctgga aaacgtgctg
     6541 gtgccgacgc tggcctttcg gcggagcgcg gcgcgcgatg gggcggctca acgggcgcgg
     6601 cacctcctgc ggcgggtcgg gctgggggaa cgcttcgccc atcgccccgg cgaactttcg
     6661 ggcggcgagc gccagcgcgt ggcggtcgtc cgcgccctga tcaaccagcc gcagcttttg
     6721 ctggccgacg agcccaccgg ctcgctcgat gacgccgcgg cgcgggaact gggacagttg
     6781 ctcctcgaac tgaatcgcga ggcaggcgtg gcgctcatgg tcgtgacgca tgcgctgagc
     6841 ctggcccgcc gcatgggacg gatgctggag ttgaagggtg gacggctggc ggcggtttcc
     6901 tgaaggggcg ggatgacctt tgggacgctc atccaccgca gcctgcgttt ccacgcgcgg
     6961 gcgcatctgg gcgtggcgct cggcgcggcg gtcggcagcg cggctttgat cggtgcgctg
     7021 gtggtgggcg attcggttcg ggaaagcctg gccggcatgg cccggcgccg cctgggcaaa
     7081 atccactttg ccctcgccac gcaggatcgc ctgttcccgg cgaacctgcg cgaacgcctg
     7141 gctggagcaa agccgccgga ctcagcggac ccgtccgccg cggccgcgaa tcgagccgtc
     7201 ccggcgcttc tgtctgcggc gctgaccctc cccggcatcg caacccgccc ggacggcgcc
     7261 gcgcgcgtca accacgtcca ggtgctcggc gtggatcctc aatcctggcc gcgcctggcc
     7321 cactggggct cgttgtcccc cgacgccagg cggcggggtc ccgcgcgcga gcccccgaac
     7381 cgcggcggcg tggtgatgcc accgcccgaa gaagaggaag aactgcttgc gcgctggcag
     7441 cgcggcggca tggcgttcat caacccagcc ctggcccgga aactggcggt ccgggcgggg
     7501 gacgaactga tcgtgcgcat gcgcaagccc agtgccctgg ggttggacgc ggccctcagc
     7561 ccgcgcaacg aggacgccgt ggcgctccga ttgaaggttg gcgcaattct gccgtccgag
     7621 cggctgggcg actttgccct gaccgcccag ccgggcccgg cggccaacct gtttctgccg
     7681 ctggactttc tttccgcgca actcggcgtc ccggggcagg cgaacctgct ggcggccggc
     7741 ccggaatggg atttgccccg gctcggccgc gaattggcgc gcgcctggca gccggaagac
     7801 gccggcctga cggtgcgcgt ggtggccccg ccgccggaca ggacgggcgg cgagcgcgtc
     7861 ccgcccttcg tcgagatctg cagcgcccgc attttccttg agccctccat cgtcgccgcc
     7921 gcgctcacgc cccgggcgga ttggcggtcg gacggaaccg gggaatccgc ccgcggcccg
     7981 ctcgccacca acggcatgcg cgtgctgacc tacctggcca acctgatgcg cgccgggaat
     8041 cgggccaccc cgtattcgat ggtgacggcg gcggaggcgc cgctggtgcc gccggatatg
     8101 cgcgacgacg aggtgctcat taacgaatgg ctcgccgacg atctgcaggt gaagcccggc
     8161 gatgccgtgg cgctgagcta ctatgtcgtg gattcgggca gccgcctcgt cgagcgcaca
     8221 aactccttcc gcgtgcgcca ggtggtgccg ctgcgcggcc gctatgccga ccggacgctc
     8281 atgccggagt ttcccggcgt ggcccaggcc gagcgcacgc gcgactggga tgcggggttt
     8341 ccgctggttt acccgattca ggcccgggat gaggcgtatt ggaaaaccta tcgcggcacg
     8401 cccaaggcct ttgtgaccct ggctgccggc caggcgatgt gggccagccg ctttggaacg
     8461 ctcaccgcca tccggtatcc cgtgccggac gataccccgc ccgacaccgt ccgcgaagcg
     8521 gtgaaacgcc agctgcggac cggcttgcgc ccggacgaag ccgggctgca gttcgaggcc
     8581 gtccgcgcgg cggcgctcaa agcggcggcg cagggacagg acttcgggct gttgtttctc
     8641 gggtttagct ggtttgtagt cggggcggcg ttgctgctga tggcgctgct cttccagttc
     8701 gagctggaac agcggtcggc ggaggtcggg attttgctgg cgctgggcct gacgcccagg
     8761 caggtgcggc gtttattgct gttcgagggc gccgcgctgg cgctggcggg cggcgcgatc
     8821 ggggccctgg gagggctggg ctatgcctgg gccatgatcc aggcgctcac caccgtctgg
     8881 cgcggcgcca tcgggagcgc ggagcttcat ttccaggcaa ccgccgccag cctgctcacc
     8941 ggtttctgcg ccagcaccgt cgtggcgacg ctgacgatct ggctgacgct gcggcggcag
     9001 gcccggcagc ccgtgcgcgc actgttaacg ggggaaatcc caagccccgt cccccgccac
     9061 cgaaatcgcg gcccgtgggc cgccgcgggc gcgggcgcgg cagcggcggg gttggtgagt
     9121 tgggcgctgc tgagccatcg gcaggcggaa ccgggcgtat tcttcggcgc gggagtgctc
     9181 gtgctgctgg cggggcttgc cgcggcgtcc gattggctgg ggcggcgggc cggggcggcg
     9241 gatgcggcgc ggttgacgct tcccggcctg ggcgcgcgcg gatgcgcgcg ccgccggaac
     9301 cgaagcctcg cctgcgtcgc cctgctggct tgcggctcct ttgtgctgct cgccatcggg
     9361 gtcttccggc tggaggcgca gcccgacgcc gtccggcgcg attccggcac cggcggattc
     9421 gccttgatcg gcgaatccac gctgcccgtt gtggcggacc tcaacacccg gaccggccgg
     9481 gatttctacg ggctggatga cgcgccgctg gccggggtga acgtggtgcc gctgcgcgtg
     9541 cgcgagggcg acgccgccaa ttgcctcaat ttgaaccgcg cccaacggcc gcggctcctg
     9601 ggcgtgaagc ccgagcggct ggcgggacgg tttgccttca ccccggcggc cggctacgac
     9661 cggcgctggg gctgggcact tctctctgcg aacccccgcc ggcccggggc gctccccgac
     9721 gcggttcccg ccatcggcga cgccaactcg atccaatggg cgctgggcaa aaagatcggg
     9781 gacacgctgg actacaccga cgagctggga cgggcgttca aagtgcggct ggtgggcgcg
     9841 gtggcaaact cgatcctgca aggaagcctg ctgattgatg aagccgagtt cacccggcgg
     9901 ttccccggcg aaagcggcta ccggatgttc ctcgttgacg cgcccgcgga cgcggtgccg
     9961 cgggtttcag cggtgctgac acgcgccttg caggatgcgg gcctggagtt gacgccggcc
    10021 gcgcggcgct tgaacgagtt caacaccgtg cagaatacct acctgggcac ctttcaggcc
    10081 ctgggcgggc tgggcttgct gttgggcagc gcgggcctgg gggtggtcgt cctgcgcaac
    10141 gtgctcgaac ggcgcggcga actggggctg ctgggggcgg tgggattccg gcggcggcag
    10201 ttgcaatggc tggtcatggc cgaacacggc gtcctgctcg gattggggct tggcctgggc
    10261 atcgcggcgg cgatggtggc ggtgctgccg gccttgctga cgcccggccc cccgcggtcg
    10321 tatggctggc tggcgctgac cctcgccgcc gtcctgctca acggcctgct gtggacctgg
    10381 ctggccgccg ccgccgccct gcgcggcaac ctgctggcgg cgctgcgcaa cgaataggcg
    10441 tcctaccccg ccagcggcgg cttctgctga tgccactccg tgctctgttc gtacgcgtgg
    10501 cccagccgca ggagggtctc ctccccgaac ggcttgccaa gcaactgcaa ccccaccggc
    10561 cgccgtggat tgccctcggt gaacccgccg ggcagcgcca aaccgcaaag gcccgccaaa
    10621 ttgcaggaga tggtaaagat atccatcagg tacatttgca gcgggtccgc ggtcttctcc
    10681 ccggccggaa acgccgtcgt cggggtggtc ggcgcgagaa tggcatccac cttgtcaaac
    10741 gccgcctgga aatcctggcg aaccagcgtc cgcacctttt gcgcccgcag gtaataggcg
    10801 tcgtagtagc cgctgctcaa cacgtaggtg cctaagataa tgcgccgctt cacttccggg
    10861 ccgaaacccg cgccgcgcgt ccggcagtac agctcggtcg gatctgaccc gctcacgcgc
    10921 aggccatagc ggatgccgtc aaaccgcgcc aggttggcgc tggcctccgc cgtggcgatg
    10981 atgtagtagg cggcaatcgc gtaatccgta tggggcagcg acacctccac caactccgcc
    11041 cccaggcggg tgaactgccg ctccgccgcc tcgagggctt gccgcaccgc cggctccagg
    11101 ccgccgaccc gatactcctt caccaggccc agcttcagcc ccttgatgtc gcccgtcagc
    11161 cccgccgcat aattccccac cggcagcggc gcgctggtgg agtcgcgggg atcatgcccg
    11221 ctgataaccc ccagcaccgt cgcggcgtcc cgcgtgtcct tggccaggca gccaatttga
    11281 tcgagcgacg acgcatacgc cgccagtccg taacgcgaca cccgcccata agacggcttc
    11341 agcccgacgc agccgcaaaa cgacgccggc tggcgaatcg agccgccggt atcggaaccc
    11401 agcgccacga tgcactcatc ggcagccacc gccgcggcgg atccgccgga ggaaccgccc
    11461 ggaacgcgcg acggatccca cggattgcgc gtaacctgga aggccgagtt ctcggtcgaa
    11521 ctgcccatgg caaactcgtc caggttgagc cgaccaaaga tcaccgcgcc ggcggccttg
    11581 agcttttcaa tgacggtcgc gtcgtaaggc gagatgtatc cttccagcat ccgcgagccg
    11641 caagtcaggg gctggccctt aacggcgatg gcatccttga ccgcgagcgg caccccgagc
    11701 aggggccgct gcgcgtgcgt ctggccggcc gccagcgccc ggtcggcttc cccggcctgc
    11761 gccagcgcat ccgccgcgtc ataactgata aacgcgcgga tgaggccatc cacactctga
    11821 attcgatcca ggcacgcctg ggtggccgcc cgcgccgaaa cctcgcgccg ggccagcttc
    11881 gccgtcagct ccgaaatggt aagttgattc aacatgcatt cgccgccgcc cgcaccaggg
    11941 ccgcggattt gcgccatata gtggcgcacc cgcgcccgct gtcaatgacg cgaacatccc
    12001 gaaaaatggc gcgccgcccc aggtcccgcc ccggcgccgc ccggcgcgat gcggcagtca
    12061 tggccgcgcc ccgggaggcg ccgcattggt gcgggcggcc gcgccgccgg gaagtggctg
    12121 gccccggccg cccaggacct cctccagccg cgccgccagc cgccgggcca taagctcatt
    12181 gccctcgtcg ccggggtgcg ccgcgtcggc cagcagcgcc ttgtcgggtt cgccaaataa
    12241 ctcaagcccc tcgaccacaa acaggcgcgg gtcgccggcc tgccggcgtt cctccgccgc
    12301 ccggcgcatc agcgcgcgca atgcctccga ccgctgccgg tagtcgggct gggccgcttc
    12361 ccgggtcgag tagatcggcg tcacgcagaa aatgggcgcc gtgggatgcg cggcgcggat
    12421 cgcgtccagc atccggttat accgctccgg ctccggcgcg ccgtaggact tgcccaggtc
    12481 aaacaaatag gccccggcgt ccagttggtt gaccagggcc accacctccg gctcggcctt
    12541 gccggcgccg ccgaagccca ggttgacaaa gtcaaggttc agccgccggg ccagggcggc
    12601 cggataggtc tgcgcgggat gcagcgcccc ggttccctgc aggacggacg agccatagca
    12661 gacgaggggg cgcggctgcg cgaacggcgg cggcggcctc agctcggcgt cggcgtccaa
    12721 gcccaccgcg aagacccgca cctcattccg gtgcggcagg tagagggtga tgtccttcca
    12781 ggcccggccc tggttggcga agatcacgcg gtccacccgc tgcgtgccct gcgggctcac
    12841 cgaacccgcg tattggccgt tcacgaaggc atcgaagcag caccgcttcc cgccgggctg
    12901 gacggcggcg aggcgaaggc ccaggcagga actgttgcag gaaagccgga tgcggccgcc
    12961 gccgggcgat accgcccggt tccagacgcc tttgggcacg cgggtttggg cgcgcttcgg
    13021 cagccgccac aagtccggcg cattctcctt gaaccacggc agcccgcata tttccagttg
    13081 cggatgcggc agatcgagcc accggaccac gccgttggtg ggggaggaat ccgccgccac
    13141 gctgaccccg cccgccagcc aaaggctgac ggtcaggccc gtcagccacc gccggcgccg
    13201 ggccgccgcc gcccccggga agccaccgtc ggaaagccgc acggccgcac cggccttttt
    13261 accacgattc tgcaccggac attattcgtt taacaggctt cccattctgc gctaaactgt
    13321 tcccatgcgc tatcgaattc taggcagcac ggggctgcgg gtgtcggtca ttggcctggg
    13381 cacctggcag tttggcggcg agtggggccg cgctttctcc caggccgacg cggacgccat
    13441 cctcgatcag gcgggcgcgc tcggcatcaa cctcctcgac accgccgagt gttacgggga
    13501 tcacctctcc gaaagcctgg tcggcgatta cctcagccgc cacgaccggt cgcgctggat
    13561 tgtggcgacc aagtttggcc accgcttcca gcgattcatg acccgggcgg aagacttctc
    13621 gcccgcgggc gtgcgccagc agcttgaagc ctccttgcgc gcgctgcgcc tggaggccat
    13681 agacctctac cagttccatt ccggctccga cagccagctc ctgaacgacg acctgtgggc
    13741 gatgctggcc gaacaaaagc aggccggcaa aatccggcac ctgggcatct cgatcccggg
    13801 caaaggcagc gagccgcagg cccgcgaggc gcggcggctt ggcgccgaga cccttcaggt
    13861 gatttacaac cgcctcgacc gccgccccga gcagctctat ttcccccacg cgcagcggga
    13921 ccacctcggc atcctggcgc gcgtcccgct ggccagcggc ttgctcagcg gcaaataccg
    13981 cgccgacgcg gccttccccg cccaggactg gcgctcgacg ctcgacgccg acaaattgcg
    14041 ccgcgacctc gccgaagtgg agcgcattcg ccagtcggag ctgcccgccg gcatggcgat
    14101 ggcgcagtgg gccctggcgt ggtgtttgca gcatcccgcc gtctccacgg tcatccccgg
    14161 ctgcatgaac ccggcccagg tggccgccaa tgccgccgcg gcggaactgg agttgccccg
    14221 gtagccgctc cccgcgcgct caaaccccgg cggccggccg gcggcccgcc cacccggcgg
    14281 cgaggtttaa cagcgccgtg accaggcagg cgccccccac gcccagcagc ggaatcagca
    14341 gcgcgcaggc cagcagcgcc cccaggcagg cgccgatgaa atcggccgca tacaaccgcg
    14401 cggccccgcg aacggtgccg tcatattccc gccggttggc cagcgggaac tgcatcccga
    14461 ccagcagcgc cagaaccagc gtcagcaggg cgatgccggc cttgaccatc acccgcgacc
    14521 ccgccgggcc gcccatgcga ttcaacagcg gcagcagcag cggcagcacc gcggcgtagg
    14581 ccgcgaggag aaacgccagc cacgacaagc cgttcccccg cccgttcgca agccagcgga
    14641 tgaatctccg gcccgggcaa cccgccagca tggccatccc cgcatccgcc gccgcgcccc
    14701 aacgccggga cgcgccggcc atccggatcg ccagcaaccg cggagccggc tgggaagtca
    14761 gcacgcccgg cgggtggagg tggggagaca ccgccagccg gttcatcagc gcggctccca
    14821 gcgccaggcc cgccatgaaa accgtcacga tgatccccaa ctggtggtaa accgagccgc
    14881 agaggacctg gaaggccagc agtaaaacca gttccagagc ggaagcggca aagcccgaag
    14941 caaacaacac cagcgcgctg ccgcgcaacc gcgccacgta atagccaagc cccagcagca
    15001 gcgccgcctc caccagcccg aaccgcgcat caaactggcg cagccagtgc cgcaggtggt
    15061 agtagtaaag aaccgggctg aagtcccggt tcaccgccgc cggctggacc gtggcatcgg
    15121 ccagggcctg catccggtcg ggcgtcagca tggcggacag gtaatggcgg ttcaccagcc
    15181 gggtcgggat tccccgcggc tccaggcgcg cggcaatgtc ggcggataac ggcccgtccg
    15241 aggccaggaa aaagacccgc tgcccgggaa tcaccagcac attgcggaac gattgctcca
    15301 ccgtccgccg cgcggaggac agcagccgcg ccagctcggg gctgacgtag ttctcgtact
    15361 gccccagcgc aaagcacacc acgcccccgg gagccagcgc gcccttgacc tccgcgaaat
    15421 actccgccgt gtaaaagcgg ttcaactggg cgctggccgg ggcgggcacg tcaataatgg
    15481 ccacctcata cttctccgcc gcctgccgcg tgaacagccg cccgtcggtg ttgctcaccc
    15541 gaatccgggg atcatcaagg ttctgcggca gatacttccg gcccagcgcc agcaccagcg
    15601 ggtccagctc cacgtaatcc accgcgccga cgccatactt gaggatttcc cgggcggtgc
    15661 ctgaaatgcc gccggacacc agcagcaccc gccgcgccgc gggccgttgc gccagcgcgt
    15721 aatgcaccgt ctcctcgacg cgctgatcgt cgcgggtcga ggcgaccggg atgccgttct
    15781 cgaggaaatc atactgcccg gccgagcggg tcacgacgag ccggccatac ggcgaattgg
    15841 catgggccac aacctcctgc cgcggatact gccgggcggt ggaccagccg tccgggtcgg
    15901 ccagcagcgt gacgccggcc aggcccacgc ccagcaccgg caccgcggcg agcagccacc
    15961 cgcgcgcggc gcgccccgcg cccggggccg gccaggccac cagccacgcc agcgccaggt
    16021 tcaaccccgc cggaacggcc agcagcgcta tatggtccag gaaccgcgcc agcacaaagc
    16081 tgaaccccac gccgcccacg atgctgccca ggctgtcggc cacataaacc cgcccaatcc
    16141 cccgggcgcc ctcgacacgc gaccggatcg agcaggccag cgtcaaacca taccccgcca
    16201 ccaggcaata cggcagcaac agcgccagcg ccgcccccgc cgtctccgcc atgcccaccg
    16261 ccgcgccccg gggaaacacc acgttgcgca gaacccgcac cgccagcacc tgcagcggcg
    16321 gcagcaccgc cgccaggatc aggacccggg ccagggcggc gagcgggtcg cgcagccgct
    16381 cggcgcggcg ccccagccgc gcgccgattc ccatcagcag gagccaccag cccagcgtca
    16441 cccccagcac catctcattg ccggcgaaca cccccagcaa ctcgcgcatc agcgccagtt
    16501 gcgccatcac gcacgacgcg cccagcgccc cggccgctgc cagcacccgc ccccggggat
    16561 caggagcggg gcggctcttc cgcgaagcac tccacgtgat agatggaaag agtgacatcc
    16621 ttgccccgcc acgcggaggc gtcgccgccg gccttccgcg aaagctgttc caggaactgg
    16681 accttgtcgg gcaactgcgc ccagacctgc ggcagaaacg tcgccgtgcg cgcgccgatg
    16741 cgcagcagca caccgtcctc gtgcgggcgc agcttggcca gcaaatcgtc cggcgaggtg
    16801 aacgccagcg gctgcggctc cgtcagcacg ctgatctcga tccggatctc gtccaattcc
    16861 ccggcctgga cgggcgggaa acgcccgtcg cgcagcgccg cgttgcgcgc gttctcggcc
    16921 actgcccggt gcaggggttc cagggcggtc aagtggccga tgcagccccg cagcgcgccg
    16981 cctttggtca gggtgacaaa acaggcccgc ttctcccgca gcttcggcgg cacgtccgcg
    17041 tcggcgacct cgggcaggcc gccggaggcc gtcacgctcc gcagggtccg gcgggccagc
    17101 tccagcagaa atttccgctc ggacgccgcc acttgctcgg gccccggcgc atagaaggcc
    17161 accgccgcgt accccaccac gcggttttta tcgccgctga catcgccact attgcggtaa
    17221 tcgagcactt gcgccttcca acccttctgc cgcgccaggt gcagcagcgc cagaacgggc
    17281 agtttgccgc aggcttcctg ggccgacatc gcctccaggt ccagcgcctc aatcgcccgg
    17341 aggcaacggc gatccagccc gcgcgcggtc tcgtacgcat gaaagtggct caagtcggtg
    17401 ctggcgacaa ctattgtttt gtcgtcccac tgctccgcca gcgcccgggc cacctgttcc
    17461 gggttcacct cgcccaggat catcggcagc aactggaagt ccggaaggac tttttgcaga
    17521 aacggcacct gcacctcgac ggagtgctcc caggtctcgg gcgtgtcctg gccggcttcc
    17581 ggcgcggctt tcgacgcctg ccgccaccaa tccggccgcc gcaccgggca gcgcggctcc
    17641 cgcgcaaacg gcgccacccc cgccagcgcc cgggcccgct ccgaaatcgg cgccagcccc
    17701 agcggcgtcc gataggcgtc cgcatccggc agggacacgc cttgcagcag cgcatagtgg
    17761 gaagctccca tgaccaccac cgtccgcgcg tcgcgcccgg ccagcagctt ataggcaatg
    17821 gccgccgtcg ccccggaaaa ctcgtagccc gcgtgcggac aaaccagccc cttcaaccgg
    17881 gggatgctgt gatccggcgc gctcgccagc agggcgtcaa tcatcccgga aagcttcttg
    17941 gcctcgccgg gatagaacaa ccccgccacc gccggctccc gcacccgccg cgcctcccgg
    18001 gcggaggact tggacggaac cgaacggcgg gacggttttt cctccggcgc ttccggccgg
    18061 gcgccgcacc ccaggccgat gaccagcaaa gccagcacgg cggcgacgca tttcccgcgg
    18121 ccgccccgat tcgctgcccg cgtttccacg tgttctgcca tgccgtcatg gcatacgctt
    18181 ttgccaccaa aaggcaagcg cataattctc acccgcgacc tcatccgcgt tggccagctc
    18241 ctcaaaaccg aagtcctcga ccacgtcatc ctcagcaacc cccgccacgg cccgctcacc
    18301 ccgccccgcc caccccccgc cccaacgggg cgccatgcga tagcccgggg caacgcccca
    18361 ggaacaccac ccccgctcgc ccgcccccgc gccccaacgg ggcgccatgc gatagcccag
    18421 ggcaacgccc tgggaatagc gccccctcaa ccagcccagc cctgaaaggg cgtaatttcc
    18481 tgcctccaac cgctttcgtc gccaccaaat cccaagcgcg tcggcggcgt catccctgcc
    18541 aaccccctcc ccatccgcat ccaccttcag cacatcctcg ggccgactcg gatcgtgctg
    18601 caggctcggc aacgtctcca ccagccgccc gcaccgccgg tgaataaaca accgggccgg
    18661 aatgttcccc tccacatcac ccaaccgctg caatacctcc gcccaaccgt tcacccggtc
    18721 catattcgcg ggcgcagcgg aggcttgcaa tcgaaaccgc cggcggcata gtgctggcca
    18781 tcatgcttat gtttcgtttc aacctggccc cccgccgaca acccatctcc tcgctatgac
    18841 ccggatttct tccccccttt cccgccgccg tttcatggaa cgctccgcgc tggccaccag
    18901 cctgttcggc gccgccgggc tggcgtcccc cggccacagt gacgccgcgg acgccgccgc
    18961 ggccgccgcg ccggcgggcg ccgtgccgcg cgtcaacgcc cgccagcatg gcgccaaagg
    19021 agacggccag tccgatgaca cccgggcgct gcaaagcgcc ctcgaggccg cgcggaagga
    19081 cggcccgatt tgctatgtgc cgcccggcct gtatcgcctg gcaggggcgc tgacggttcc
    19141 gccgggcgtg acgctttgcg gggcgtcggg cggcgtgccg catagtgagc acccgatcgg
    19201 cacggtgctg ctggcggtgg gcgggcgcgg ccaggccgac ggcgagccgc tcatcacgct
    19261 caagcccaac gcggtggcgc gaaacctcat catccattac cccgagcaaa cccttgccga
    19321 agtggcgccc tacccgtggt ccatccgcgc cgatggcgag ctgtgccaga tccgggacat
    19381 cacgctcacc aacccctacc aggccctcga cctgggcagc cgctggaacg aactgcacct
    19441 ggtccgcaac gtcttcgcct gccccctcaa gattggtgtc tatattgacc agtgcaccga
    19501 catcggccgc atcgagaacg tccacttcaa cccgaacttc tggacgcgca tggcgcttaa
    19561 gcccggcttc cccggcggcg acattaaagc ttacctggag cagaacctcg tggggttcaa
    19621 gatcggcaag accgactggg agttcatcag caacagcttc gtcatcttcc cccggatcgg
    19681 gtttcacttc gacgacttcg gccacggacc gggcaacgcc gtcatcacgc agtccggcag
    19741 cgacatcggc cccgtggcgg tgcaggtgga ccgttcccag ccgcacgccg gcgtccagtt
    19801 tgccaatgcc cagttcatgt ccaccctcgt ggtcggcccc aacaatcaag gccccgtcaa
    19861 actggccaac tgcggcttct ggggcatccc cgaaacccgc gaacaggtcc gccattccgg
    19921 gcccggcacg ctggccctga cggcctgcca cttcaacggc tgggaccagc cgggcaaggg
    19981 cgccccctgc atccgggccg ccggcgggcg gctgatggtc aatggctgcg agttcatgga
    20041 cggcggcaaa cgcgccatcg tcctggaaaa ggggctggtg gccgcaacca tcttcggctg
    20101 cgctttccgc ggcgcggcgg cggttgacga ccaatccggc gccgacgtgc aaatcggcct
    20161 caacacgagc cgctagccgc cccaggacgc ccgccccgga aaaggctcac cgccagtgat
    20221 ggatcccgac cggccgagag cagttccctc tcctcaaggg aaagggaact acacgcgcaa
    20281 ctgcatagac acggttaagg caagactgtc gtcaccaccg tggccgaatc attgccggcc
    20341 accgggtccg gagccgggtt ggcgacaatc gccgtattct ccagcgaacc cggttgctgc
    20401 ggtctgacgc ggatcacgac cgacaccccc ggcccgggca gcagcggatt cagcagttcg
    20461 caggtcaaca tgccatcaga atacacgcag ttaccctcct ctggcggcag gacttcagcc
    20521 gaaatgtact ccacgctcgc cggcagcgga tcgaaaatct gcaccttccg ggccggcgga
    20581 ccgcccaggt tcttcacttc aagggtgtag gtaagctcct gtcccactct caccgggttg
    20641 ggcattgcaa tttgggttat gtccatgttc acggattgca tccccactcg gtagtagcgg
    20701 ccgcccggct ccaaagtatc cttcatcaca accggttcac ccgtgccggt cggaatgtcc
    20761 catatctccc atcccggcgg gagcacctcc tcggctcgct ccaccacgta atccaccccc
    20821 aacgaggtgt cgaagcacaa ctcgacctcc atccccacct gccgggcgga aacagacagc
    20881 tcaggcttgg gactggtgat attacccgcg aaggactccg tctgatccaa tgccgtcagg
    20941 aaatgcaggc tgccgttctc caaacccagt ggcggcagga agcagccgga acaactgccc
    21001 gcggcgttta aggtcccgtt gtaaaccagc gtgtcatcca ggtaaagctg caccggctga
    21061 ttgggcgaca tccgcgtgag cgtgaacgga tacggcacgc cggcccgggg cacaccgctc
    21121 ccgtagagtc gcagatccgg ccggtgcgca tagagcattt gatcaaacgt cagctccacc
    21181 gtgtcgaaga attccaccgg aatgcccgcc gccgccacaa tgggcacctg cggcgccgtc
    21241 aacgccagca tgctcttggg cacctggaag gcaatggtgg tgccgatggg aaccgtgggc
    21301 ccctcccggt cgttgaactc aggctccgtc agcagttcga tcggccccgg caacagcatc
    21361 cccggaggag gcatcattgg gtcctccaca aacccctgca ccagcagcgg ccccggctcc
    21421 gtgggccgcg acacctccac tcgcacaatc cggtctatcc ccgcccgccc cgcatagctg
    21481 acgccggtga gaggattgtt atcggtatcc aggcccaggg cgtacctcgc cgccagcgtg
    21541 ccctcggacg gtatgacgcc gcccactccc agggagaccg taagggtcgg cacgccctgt
    21601 tccgaaccaa cccacaccct ttgcaggtcg tgaatgtcct tcacgtcggc gccgtgaccc
    21661 ccgctggaaa tcgcgtcccc atgatcatcc cgcgccgcgc ccgattgcac caggccgacg
    21721 acggccgggc aattttcctt gaactgccgc gcgctgaatc cgaatgtggt cacctccttg
    21781 ttcatctcct caatctggag cttggtcagg ttcgtggcgc caggggtgtg cttcggggcc
    21841 atcaggttct cttcgcctgc cgggcccggg ttgttcgttg acgagtgtcc atcctcatcc
    21901 gcgtcaatgc cggttgcgat cttgtgatac gcgcccaacg tgaacacatg cgccgcctcg
    21961 tgcgcgatgg tctgcgcggt gtcggtagtg ctcccatact tttgcacgat aatcgtcggg
    22021 tcgcggtgca ccgcgactcc gatgttgctc gcattggttg cgttgacctg ctggccaaac
    22081 gagattttca tccccgtgct ccccggcagt cccgccactt ccttgccgcc aaatgtgcgg
    22141 attgcgtccc gctcagcccg ggtgaacacc ccgtcgccat catccccgtt gttggcggcg
    22201 gtcacgggat cgttgatctt caccggcacc aaccggatgt tggcctggcc caggattttg
    22261 ttcgcctcgg caatggcgtc cgcaatgtat tgcgttgaca cattggcgtt ggagtaaaca
    22321 ttcacaatga cggggacatg agtgccttgc gccggggctt gcggcgccgc ggcaaatgcc
    22381 gtcacgggca acagggtcgc cgccagcaag gcgatggcga aactcagcgg cggcgcccgg
    22441 cgcgtgacgt tatgggtcca tcctgatata aacgggtagt tactcatggt gtgcagtcct
    22501 ttcttacgtt gaccgtaaaa cacgtgactc cgggcggacg cttgcggcgg gcgggtgggt
    22561 atagaagttc tcccctctgt ctttctctcc tacacccgca tggcttgcgt ggttgcagat
    22621 ccacatgttc gacagatttg ttagcggggt ggcggcccga ccatccccat tgacctccga
    22681 aatcaggctg atttcgaacc cttttcaaag ctcccgctct accaagcagg agcttcactg
    22741 acgatttcac tctcttcgca cggcctcgtt aagaataaag tgtcaaaaaa tttcaatatg
    22801 cgtattgact cacaatgcct atgactgaag aggaggaatg gggtgcgggg ggagggattg
    22861 gttgattcat accggggctt accttgccgc caggaaaacg gcaatcatgg gccgaaaatc
    22921 gaagcaagaa taccggttgg caatctgggg ccgcagccag agcgtggggc ggcggttgac
    22981 gaccaatccg gcgccgacgt gcaaatcggc ctcaacacga gccgccagcc gccgccggcg
    23041 gcggccggtc agcttttccg cccggtcaac tgctccagga acagccgggc cttccgggcg
    23101 gcggctttaa cgtccgccag cgcgccgcgg gcgagacact ccaccagcaa cggcccgccg
    23161 gtgaagccgc cctgcttcag ccggtcaaaa acctttggaa agtccacccg cccctcgccg
    23221 atgttcacca gcacgtcctt gggcggccgg taatccttta cgctcatccc catcaccagg
    23281 ccatccaccg tggccgcatc cgccaccgga tccagttgcc cgtcggaata gaagaagata
    23341 ttgcccggat cataccacag cccgaagttc ttgcgcccga ccaactcgat ggctttgcgg
    23401 cactggggcc cggtcgcgtt ccggccgccg tgcggcttga tgctgaggtt gacgcctttg
    23461 gccgcggcat gatcgcagca ctcggcaacg gtctggtagt agatttggta gcgctcatcg
    23521 ccaatcccgc ccagcatcaa gtgcggacat tcgcaaatcg ccgcatcatc aattaattgc
    23581 ttcagccccc ggcagttctc ctccagcgaa ctgctgaccg ggaattcacc ataaatggac
    23641 agcacctcca gcccgcgctt ccgcgctgcc cggtgcatat tccggacttc ctcaaccggc
    23701 gtgctggatt taatcatgac ccagcccgcg atgttggagg tcatgatccc gacatacctg
    23761 aagcccgcct cggcgatggc gtccagcgcc accgggtaat cgaactggtc gaacggccgc
    23821 gtatagcaac cgatttgcca gttgggcttg cgcgccggcg ccgcctccgc cgccagcagc
    23881 gcgggcagct tcagcaccga catccctgcc atccccattc ccgccacccc gcccagaaat
    23941 gtccgccgat tgatggggtt ggccgtattc gtcgccatac acttcgtcat gctcgcctta
    24001 ttccatcgcc aaacccgccc actggcaaga gttaagttcc tccgcccgcc taaagaaaag
    24061 gcgggggcgg cgattcagca ggcggttcgt tgaggcggtg gtccgggcgg ccaggttcac
    24121 ccggcctcca atggaacaag ggtgcgcgcc cgggtcaccc aactgcctcg ttccggccaa
    24181 accacccgga aggccaaaag gctcccgttg actcgctttt ccttgaaaaa ccgcttccaa
    24241 tccgccccag attcacaaat agctgttttt ctctaactta tcactcatac cctcgttctt
    24301 tcccatcgtt tcggcctttt caaacatccg gctccattat ccagataccg tggatttacc
    24361 ctgccatcag gccgtgtaca aggggtgtac aagggatgcc catcgggtgt accagctaaa
    24421 atccgatgga ctcccgatga gcatcccttg tacacccctt gtacacggcc tgggagcagg
    24481 gtaaaggcaa ggcaggtttg gggtaagccg aggtcggcag ggttcagatt ttgccgatat
    24541 tttatccctt ttcacgccta aacccttgat tttacaggcg
//
LOCUS       DAOPQG010000054        24179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2617_length_24179_cov_14.918339, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000054 DAOPQG010000000
VERSION     DAOPQG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 24179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24179
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2617_length_24179_cov_14.918339"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..1307
                     /locus_tag="PKI07_07815"
     CDS             408..1307
                     /locus_tag="PKI07_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOC50998.1"
                     /translation="MLLGIEIGGTKLQLVRGDATGRIHARRKLAVDRARGAAGIRQQI
                     EQALPELLRGERVRKVGVGFGGPVDWQTGRICRSHQVEGWSEFDLGGWLRQLTGAPVA
                     VDNDANVAALGETLCGAGAGHNPVFYVTLGSGVGGGLVVDGRIYHGARPGEAEIGHVR
                     LDRRGTIVEARCSGWAVDARIRELRVTEPESLLARRAGATAGGEARHLAAAWQAGDPA
                     ARRLLRETAEDLAFGLSHVVHLFHPELIILGGGLAGVGEPLRAAVAEALGAFTMAAFA
                     PGPRLALAALGEDAVPVGALKLA"
     gene            1340..1909
                     /locus_tag="PKI07_07820"
     CDS             1340..1909
                     /locus_tag="PKI07_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOC50999.1"
                     /translation="MKQWLAQYVQAQKAALDAIPLAAVAQLIEKLRVALREGRQIFVF
                     GNGGSAANASHFATDLGKLASDRIGRRFRVLSLNDNTGWLTALANDYAYAEVFAGQLQ
                     NYGQPGDLALGLSVSGDSPNCVKALEWARQHGLHTVALVGARRGRMAEIAEQAIVINE
                     THYGRVEDAQMGICHLVCYAFAEKPELAD"
     gene            2035..3294
                     /locus_tag="PKI07_07825"
     CDS             2035..3294
                     /locus_tag="PKI07_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51000.1"
                     /translation="MKLLAIEKKVARGAVRLSGRLLRGGDSKPMEAFFEYPEEFAPLV
                     VENADPFVPALLLPAMAAREPVEIPLPISRRLYRNLPLIQHILSAWYPEALTPVPVRA
                     EHFREAAPAANTGTGAFFSLGVDSFDTLLRHADGAAGGPPLTHLIYMTGFESPLRLGE
                     DGRERPVIAAVQEAARHWGKQAICGRTNLRDCFSLRWGGYYHGAGLAAIALSLARGLG
                     RVLIPAGPAWRYFVINPSNPVTDPMWSSEAQEIIYDGAEYVRAQKIALTLSQNPEALR
                     RLRVCLVTEGGAGNCGFCNKCIRTMITLEIVGALRTCGVFPPRLPRDFWRRLEVTSAG
                     ELTMVDGNLWLARERGAPRWLQRGLERAANLGRLEIVRHQRGDVRFLKDLLRLGVERT
                     YGRIRLHFYRQRLRAFKARNAPGQRGR"
     gene            3542..3889
                     /locus_tag="PKI07_07830"
     CDS             3542..3889
                     /locus_tag="PKI07_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOC51001.1"
                     /translation="MSIILKRAYDPPAKTDGQRILVDRLWPRGLTKAKAKIDRWLKDL
                     APSTELRQWFGHDPEKWAEFQKRYRAELKANPALSELRALARQGDIALIYAARDQQHN
                     EAVVLKQELERRA"
     gene            4132..4467
                     /locus_tag="PKI07_07835"
     CDS             4132..4467
                     /locus_tag="PKI07_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOC51002.1"
                     /translation="MNGYYELKTTTTGKPMFNLKAGNHEVILTSEVYESKAAALNGIE
                     SVRKNGVNAAAFEKKMSVKNEPYFVLKAANGQVIGKSEMYSSEAACDNGIASVMRNCP
                     SEKIVEVAA"
     gene            4728..8492
                     /gene="metH"
                     /locus_tag="PKI07_07840"
     CDS             4728..8492
                     /gene="metH"
                     /locus_tag="PKI07_07840"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOC51003.1"
                     /translation="MADEQNRTARAQELKRQLRERIVLLDGAMGTMIQAHHLDEAAFR
                     GERFRDWPRDLKGNNDLLNLTQPRIIRAIHRQYLEAGADIIEANTFNSTAISLADYGL
                     EELAYELNVAGGRLARAEADAVMAAHPGRVCWVAGALGPTNKTASLSPDVHNPAFRAI
                     TFDQLVAAYTEQARGLLAGGVDLLLLETVFDSLNSKAALFAIAQLFEESGQRVPVMVS
                     FTITDLSGRTLSGQTVEAYWNSIAQHELLSVGINCALGPREMRPFIEELARIANICVS
                     AYPNAGLPDPMQPTGFPETPETLAPQLREWAANGWLNLVGGCCGTTPAHIRALAEAVR
                     GLPPRVPPPIAPATRLSGLEALTIRPEANFVNIGERTNVTGSPRFAKLILAGNYEAAV
                     AIARQQVENGAQIIDVNMDEGMLDSETAMTTFLNLVAAEPDIARVPIMIDSSKWSVIE
                     AGLKCLQGKGIVNSISLKEGEANLIGQAKRIRRYGAAVVVMAFDERGQADTFARKIEV
                     CERSYRLLTQQAGFPPEDIIFDPNVLTLGTGIEEHNNYGVDFIRATRWIKEHLPLARV
                     SGGISNISFSFRGNNAVREAIHSAFLYHAIRAGLDMGIVNPGLLEVYEEIPQPLRELV
                     EDLILNRRPDATERLVRFAEGVKPKDETAAAEAEWRQGTVEQRLSHALIKGITDFIDA
                     DTEEARRKYGRPLQVIEGPLMQGMNVVGDLFGSGRMFLPQVVRSARVMKKAVAYLLPF
                     MEAEKAAAGARPAQGRILTATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPAEKIL
                     QSARELKADLIGLSGLITPSLDEMAHVAKEMERLGFRLPLLIGGATTSKIHTAVKIAP
                     AYTGPVVHVLDASRAVSVVNSLLDARLKPAYLEGIQADYDKLRAQHAGQMTKPLLSLD
                     EARRRRHPLEWQAGDLLRPAFTGWRVLASDPAARGPQPVSPIALADLVPFIDWSPFFH
                     TWELRGRYPEILQNPEAKKLFDDAQALLREILRRPALVARGVYGFFPANAVGDDVELY
                     TDDSRTEVLATFHFLRQQMDKPASQFNHCLADYIAPRPPERGEPLPDYLGAFAVSAGF
                     GTEALCQEFERAQDDYNSIMTKALADRLAEAFAEFLHQRVRQEWGYGRDENLSYADLI
                     RERYRGIRPAAGYPACPDHTEKQTLWKLLDVEKNTGIKLTESGAMWPGASVSGLYFAH
                     PESKYFGVGKLGRDQVLDYHRRKQMELAAVERWLGPYLNYDPAAFGAARP"
     gene            8547..8999
                     /locus_tag="PKI07_07845"
     CDS             8547..8999
                     /locus_tag="PKI07_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HOC51004.1"
                     /translation="MKNDVLPVQIRGILPANSGCALFVGNEEKVFVINVEPNMGAVIG
                     MFLRQTPKERPLTHDLIQSIFKGFSINVERVVITEMKNSTYFARLILQQQNELGRKIV
                     EIDARPSDCLALATAQKRPIFVTHSLFEQVEDMSEVLERINENDSESE"
     gene            9004..10191
                     /gene="holA"
                     /locus_tag="PKI07_07850"
     CDS             9004..10191
                     /gene="holA"
                     /locus_tag="PKI07_07850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOC51005.1"
                     /translation="MPPPAAQPAASLKLVCGEDDFVVKQRARQIHQQWTAEVGGMDHE
                     TIDASVTTKDEAFRALAKLREALQTLPFFGSGKVIWFKDCNFLSDERAATPDVTEALA
                     ELARELKEFAWANVRLLISAGKVDKRKAFYKTLDKLGAVESFAGWSADDPDWAEQAEA
                     WARQALRARQKEISDEALAELVSRVGPHSGLLDSEIEKVSLYVGERREIGRADVAAIC
                     TRNKTARAFALGDALGDRDLPRLLARLDEALWEVKLDRQKTEIGLLYGLIYKVRAMLL
                     LKELLREGWIQPDANFSQFKKQLERIPAGQLPEDKRFNPRSLHPFMLFKALPQVKRYR
                     PAELVRAMDLLFECNLRLVSSGLEESLVLQQALVQIVSPPASPPRPEAARARPAAVAG
                     ARR"
     gene            complement(10216..12297)
                     /locus_tag="PKI07_07855"
     CDS             complement(10216..12297)
                     /locus_tag="PKI07_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC51006.1"
                     /translation="MSREPALPPFGAPVPLQIDYARELNAQQYAAVTAPPGPSLVIAG
                     AGSGKTRALIYRVAYLLEQGIPPNRILLLTFTNKAAQEMMRRTADLLGRGLAALWGGT
                     FHALGNRVLRRHADQLGYSRDFTILDSEDARRLITTCMTEAEVNAKGGKFPKAEVLAD
                     LFSLAVNTRQPLSAVLRDQHARFARLEPQIRDLQQRYAARKRAINALDFDDLLAQWLR
                     LMREHPEIGEHYQRRFQFILVDEYQDTNPLQGELIDLLAARNQNVMAVGDDAQSIYGW
                     RGASCLNILKFPERYPAAKVYKIETNYRSTPEILRVANAAIAANVHQFAKKLAPARPA
                     GGKPWVVACADATEQGAFVAQRVAQLRAEGGRLSRMAVLYRAHFHALELQLELTHGDI
                     PFIITSGIRFFEQAHIKDVIAYVKLVANPRDELSFKRLALALPGIGAKGADKLWRAFA
                     AGAPTPPPARPAAPPADGPDAPGIPPPEPSLPLAARLRACGRVVPKKAAAAWAQLAAT
                     LEQLAAEDVRDDVAAMIGLVMKAGYAEYLASNYTNPETRLEELEQLAIFARQFPTTTD
                     FLTQLALLTNLEAESESPARADEDRLRLSTIHQAKGLEFDVVFVIMLCDGLFPLERAL
                     ASPAGEEEERRLMYVAITRARNELYLCYPRQRAAYGSGGGLAQWPSRFLQEIPRKLLK
                     EWELPSFERDE"
     gene            complement(12341..13639)
                     /gene="lysA"
                     /locus_tag="PKI07_07860"
     CDS             complement(12341..13639)
                     /gene="lysA"
                     /locus_tag="PKI07_07860"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOC51007.1"
                     /translation="MHHFHYSGNRLYCEGVAVESLIRKYGTPLYVYSQRTLTERYQAL
                     AQALAGVDSLICFAVKSNSNLAVLRALADLGSGFDIVSGGELERVIAAGGDPRRCAFA
                     GVGKTESEIEFALRRGIYTFNAESEPELERINRVAARLKRRAPVAVRVNPNVEARTHK
                     KITTGTYENKFGIAFEQIEGVYARAARLKHLWLRGLQMHIGSQITEVAPFEQAVRKVR
                     PLVARLKERHGLEFFSIGGGLGIVYQPALASGPAAWWRSSAARGMITPQQYAARLQPL
                     LQPLGLKILLEPGRFISGNAGLLVTRIEYVKRTGRKNFLIVDAAMNDLIRPAFYEAYH
                     EIVPVRRRAGAQIRADVVGPICESGDFFCQDRPLPKAEAGDYLALLSAGAYASVMASN
                     YNTRSLAAEVMVKGRQAALVRARQPVKAIWAGEQIAPWQR"
     gene            complement(13706..14545)
                     /locus_tag="PKI07_07865"
     CDS             complement(13706..14545)
                     /locus_tag="PKI07_07865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOC51008.1"
                     /translation="MVNKHCRLAAGLLALAGAGALNFSSARAQPASGVVAAVPAPAGP
                     GVELREGGIIRGPTSARRLALVFTGHKYAEGGPQILDELARHKAKGSFFFTGKFLTRG
                     EFQPLIRRLVAEGHYLGPHSDRHVLYCPWDGPRKTLISRAEFRADLERNLKKIERCGV
                     KRAQVRYFLPPYEHYNQEIVDWSAELGLTVVNFTPGTRANADYTGEADRNFVSSEIIF
                     NSILRKEQQDPRGLNGFLLLMHIGAGPGRTDKFAARLGELLDYLAGKGYAFVRVDDLL
                     AAR"
     gene            complement(14614..15864)
                     /locus_tag="PKI07_07870"
     CDS             complement(14614..15864)
                     /locus_tag="PKI07_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51009.1"
                     /translation="MAGTEQSGAVGGSAGAPGLLARRQFLTRTLGFAAGMSLPGLARR
                     ARGAEAPPFRAAIIGHTGRGNYGHGHELIFTGRPNITVVAVADPDEPGRRQAAARAGA
                     RRSYADYRQMLEREKPHLVVVAPRWTGEHHAMARAALSAGAHVYLEKPITQTLAEADE
                     LLAAAARGGLRIVVAQQVRLAPNILFLKQCLADGLMGELLEMRAQGKQDHRAGGEDLV
                     VLGVHLFDLLRFFAGDPLWCAARVRQGGREITPADAHPATEGIGPVAGDDLCAQFAFP
                     QGVNATFTSRIQNRELAEPWGLELIGARGAVKILMDMVPRIYVRKGGSWTAQGQAGEW
                     RVWEQDPTRGLSREQRSMAAANQRVVDDWLAAIADRREPLCSGYAGMKALEMAMAVFA
                     AGLARQRVAFPLANRNHPLRAPGS"
     gene            complement(15874..17223)
                     /locus_tag="PKI07_07875"
     CDS             complement(15874..17223)
                     /locus_tag="PKI07_07875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOC51010.1"
                     /translation="MRAKQVLLFTLIAGLAGLAQASAPQTAEALRQLYQPAKAPAPKR
                     LLLRPGDRLAICGDSITEQKMYSRIIEDYLTVCVPELNVSVRQYGWGGERAPGFLARM
                     TNDCLRFRPTLATTCYGMNDHEYRAYEDRIGQTYREASTAIVRAFKARGVRVIQGAPG
                     IVGKVPGWTRHTNDTRLDLNRNLGRLRNLGIEIAKAERVGFADVFWAMLTADATARQV
                     YGPGYALAGEDGVHPGWAGHLVMAYAFLKGMGLTGDIGRFEVELKKNRMTLSPGHRLV
                     SAREGEFVIQSSRYPFCACAPAGLGGASYPVCGADDPATDSSIRSALKLVPFNEELNR
                     LTLKVRNVRSGRSYRVTWGAEARTFTGEQLRRGVNLAAEFARNPFSEAFARVDAAVAA
                     KQAYETTQIKTRFRSPEAKADMEAVAAQTEREREPLAAAIRAAFVPVTHTLRIERAP"
     gene            complement(17566..18711)
                     /locus_tag="PKI07_07880"
     CDS             complement(17566..18711)
                     /locus_tag="PKI07_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOC51011.1"
                     /translation="MKFSAVSAPAPGGGGVSRRSGWGWRAGGWGAALLLLAGCCSTPR
                     GKPLQRYEFTHPAMGTLMRITLYAPDPGAARAAAGAAFQRIDALEDILSDYQADSELN
                     RLRDHPSGTPVPVSAELFDVLQRACEVARLSDGAFDPTIGPFVRLWRFSRKRGVLPTA
                     AEIAAARAAVGWRKLALDPRARTVTLRVPGMRLDLGSIGKGYAADQALRLLKERGINR
                     ALVAASGDLAIGDPPPGRRGWRVALAGFAGDTNGLPAPLRLRNAGVSTAGDAEQFIVI
                     DGVRYSHIVDPATGFGLTRRIQTTVIGPDATTSDSLDTAVSLLGVKRGLALVDSWPRA
                     AALILTQENGRTQSVASRRFRKLAPSWPASSPAMRGRPRRGLPDPAP"
     gene            complement(18782..20119)
                     /locus_tag="PKI07_07885"
     CDS             complement(18782..20119)
                     /locus_tag="PKI07_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51012.1"
                     /translation="MPDHLSSSSTAVSRRQFLKHSTVLAASAAAAASFPAVLHAQSPR
                     VINAVIIGLGGRGSGAGRDFLEAAKAAGVEGKIAGVADIFPDAARKGVENFGLSEDKC
                     FSGFDGFQKALEVPNVQYAILATPPGFRPAYFQAAVAAGKHVFMEKPVAVDGPGCRIM
                     YAAAAAARQKNLKVVAGTQRRHQGGYVETVQRLRDGAIGDIHTLRAYWVNGGPIWHRG
                     DHGATELEKQIRNWYHYIWLCGDHICEQHVHNIDVCNWIVGDHPVKCWGMGARQQLDG
                     KSGEIWDNFAVEYEYPNGARMFSYCGQIKRAWSSVSEAVQGTRGTADPGGRIQPQGGA
                     AWRYRKTGAEVSPYVQEHIDLINAILRDADLNEGKQVTDSTLTAIMGRETAYSGAAGD
                     WEAALNSTFRYGPDLLYTEPAKMTWGAFRTLQPPMPGLHDIYKDPPSVPTVQI"
     gene            complement(20191..22359)
                     /locus_tag="PKI07_07890"
     CDS             complement(20191..22359)
                     /locus_tag="PKI07_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOC51013.1"
                     /translation="MKYYASLAAVLSFLSLAPLPLPAESAAPALIYETPQEFFAGGDF
                     DRDGRADLVIVDKSSGKFRLGYQLAPGVFTWVDCRPSGLKDVTGFSLGPLLKKDQEAL
                     AFTSPDANEITLVDVSSATAPSKPVALPFTAALGPNTIVAVDVGGAGNTELADLAVGS
                     IYNSPDPNLGVLLRNDGAEFPKLAEETLPGAAVRGNRVALKAGSRELVCGLVQGDNGD
                     MLRVLDVSGAKPVVVASAEGLPAGSDYTVGRFRGSPLCEFIVYKPGESRVTVRPVEEP
                     AAGTFRFGAGASFDLGQPVRRVVALEGGGARKLLVIFGEGEKAGLYDFDGTRAPALAH
                     RLPANNDLFTCAAAAPGGFIVFSQPAGGKFSTRYQMFKVSGGACAPGAAGSLASLADN
                     DNVIIPDIHARIAAQPAVAGPEGMKPYTNTIPGTQVSFVMVPIPGGEFVMGSPAGEAG
                     RKPDEGPQHKVKIAPFWMGQCEVTWNEYELFMYPDEERRTRATLPTDAAGDKLADAVT
                     HPSRPYVEMSFGMGKEGFPAIAMTQHAANKYCQWLSAKTGQFYRLPTEAEWEYAARAG
                     TTTAYFFGDDPAKLGEYGWYAENSDFKYQKVGKKKPNPWGLYDMCGNVVEWVLDQYAP
                     DYYQKCAAAGVSVEPWNKATQPYPHSVRGGSWDDEADQCRSAARRGSDRAWKMQDPQL
                     PKSIWYFSDAQWVGFRLVRPLQVPTIEQMQKYWTSGVEKD"
     gene            complement(22478..23953)
                     /locus_tag="PKI07_07895"
     CDS             complement(22478..23953)
                     /locus_tag="PKI07_07895"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOC51014.1"
                     /translation="MSQSLLYHAFGVREGYEYVRTEYRRGTAGFHLTVAPCLMVCPHC
                     QGREIIRKGKRFRRIQTVPIGFKAVFLNEKLDELRRVRVREAVGTRRQATKGTRYYSR
                     WSWLRRRGIFAAALFSTIMRQITDKAVVALLGCLLAAAMVSGQAAEPDGGWQWHDARG
                     LVLEGKGWRDTRQYYDRLPGRAEGIVRGAVWGLSQDSAGLAVRFVTDATAISARWTLR
                     SERLAMAHMPASGVSGVDLYVKDKGRWRWLGAGRAEKYPTEEKTLARELPPMRREYLL
                     YLPLYNGVTEVQIGLPAGAKLEAAPAREPGRKPVVFYGTSILQGGCAARPGMAYPAIL
                     GRRLDREAINLGFSGNAWGEPEMARLLAELDPAAYVLDPLPNMKEEWVAPRLREFIAI
                     LRKAHPRTPIVLVEHAVYPDSESLGPQAGRCARSNARLRELHRQWVRAGDRRLWYVSG
                     AGLTGDDREGTVDGVHATDLGFYRMAGAIEPTLRRALKFHR"
BASE COUNT         4034 a         8377 c         7974 g         3794 t
ORIGIN      
        1 aacattggcc aaagggcgtc gagtaataga cataagctcc gaggcgtccg gagcgcgggg
       61 aatcgagtaa tttcataagc gagaaacttc catggttatt gtaatccaag gccgggagag
      121 cggcccggcg gcccgcgcac gcgggtcagt taaccgggca gcggtttgtc cccagccacc
      181 ggaaaaaggg tatcagaccg gaggcggcgg tcaacgaaga tagcgttctc ttcaaaatgc
      241 gagaacgcgc ccggtttccg ccgccggctt tgcggccgcg cgggggaatc ctccgccctg
      301 agcgttgaga gagggagcgc ggacgcggcg ggttggccgc cgccacgttt ccggcggggg
      361 gctttttcct tttgcctttg ttcttcagcc ttcggcatcc tcccggcatg cttctcggga
      421 ttgaaattgg cggcaccaag ctgcaactcg tgcggggaga tgcgacgggg cggattcacg
      481 cgcggcggaa gctggcggtt gaccgggcgc ggggggcggc gggcatccgg caacagattg
      541 agcaggcgct gccggagttg ctgcgcgggg agcgggttcg caaggtgggc gttggttttg
      601 gcgggccggt ggattggcag accggccgca tctgccgctc gcaccaggtc gagggctggt
      661 cggagtttga cctgggcggc tggctgcggc agctcacggg ggcgccggtg gcggtggaca
      721 acgatgccaa cgtggcggcg ctgggcgaaa cgctgtgcgg cgccggggcg ggccacaacc
      781 cggttttcta cgtgaccctc ggcagcggcg tggggggcgg gctggtggtg gatgggcgca
      841 tttaccacgg cgcccggccg ggcgaagcgg agattggcca tgtgcgcctg gatcggcgcg
      901 ggacgattgt ggaggcgcgc tgctccgggt gggcggtgga cgcccggatt cgcgagttgc
      961 gggtgacgga gccggagagt ttgctggcgc ggcgggcggg ggcaacggcg ggcggggaag
     1021 cgcggcattt ggcggcggcg tggcaagcgg gggatccggc ggcgcggcgg ctgttgcggg
     1081 agacggcgga agacctggcg tttgggttgt cgcatgtggt ccatttgttt cacccggagc
     1141 tgatcatcct gggcgggggg ctggcggggg tgggcgagcc tttgcgcgcg gcggtggcgg
     1201 aggcgctggg cgcgttcacg atggccgcgt tcgcgcccgg gccccggctg gccctggcgg
     1261 cgctggggga ggacgcggtg ccggtggggg cgctaaaatt ggcctgacaa accggcgggc
     1321 agggcggaag atggcgggca tgaaacagtg gcttgcccag tatgtccagg cccagaaagc
     1381 ggcgctggat gccatccccc tcgcggcggt ggcgcagttg attgagaagc tgcgcgtggc
     1441 gctccgggaa gggcgccaga tttttgtctt tggcaacggc ggcagcgcgg ccaatgcctc
     1501 gcacttcgcc acggacctgg gcaagctcgc ctcggaccgg atcgggcggc ggttccgggt
     1561 gctttcgctc aatgacaaca cgggctggct gaccgcgctg gccaacgatt acgcctacgc
     1621 ggaggtgttc gccggccaac tgcagaacta cggccagccc ggggacctgg cgctcggcct
     1681 cagcgtcagc ggcgattcgc ccaactgcgt caaggcgctg gaatgggcgc ggcagcacgg
     1741 gctgcatacc gtcgcgctgg tgggggcccg gcgcgggcgc atggccgaaa tcgccgagca
     1801 ggcgatcgtg atcaacgaga cccactacgg gcgggtcgag gacgcgcaga tgggcatttg
     1861 ccacctggtg tgctacgcct ttgcggaaaa gccggagctg gccgattaag ttgcgatttg
     1921 aacccgaacc gtgggcggcg gaggcgcgcg gcacggagct gaccgcgcgg cgggcgggct
     1981 tccggaatgc gggcggggcc aggcggcgcg cggcgggccg ttattaattt gtttatgaaa
     2041 cttctggcga tcgaaaagaa agtggcgcgg ggagcggtgc ggctgtcggg ccgtttgctg
     2101 cggggcgggg actcgaagcc catggaagcg tttttcgagt atcccgagga gttcgccccc
     2161 ctggtcgtgg aaaatgcgga tccgtttgtg ccggcgctgc tgctgcccgc gatggcggcg
     2221 cgggagccgg tggaaatccc gctgccgatt tcgcggcggc tctaccggaa tcttcccctg
     2281 attcagcaca ttctctcggc ctggtatccc gaggcgctga cgcccgtgcc cgtgcgggcc
     2341 gagcatttcc gggaggcggc gccggcggcg aacaccggca cgggcgcgtt tttttccctg
     2401 ggcgtggatt cgtttgacac cttgctgcgc cacgcggacg gcgcggcggg cggcccgccg
     2461 ctcacgcact taatctatat gacgggcttc gagtccccgc tgcggctggg ggaggacggg
     2521 cgggagcggc cggtcattgc cgcggttcag gaggccgcgc ggcactgggg caagcaggcg
     2581 atctgcgggc gcaccaattt gcgcgactgc ttttccctgc ggtggggagg gtattatcac
     2641 ggcgcggggc tggcggcgat cgcactgtcg ctggcgcggg gcctgggccg ggtgttgatt
     2701 cccgccgggc ccgcctggcg gtatttcgtc atcaacccca gcaatcccgt caccgatccg
     2761 atgtggtcga gcgaggccca ggaaatcatt tacgacgggg ccgaatatgt gcgggcgcag
     2821 aagatcgcgc tgaccctgag ccaaaacccg gaggcgctgc ggcgcttgcg ggtttgcctg
     2881 gtcacggaag gcggggccgg caactgcggt ttttgcaaca agtgcatccg gaccatgatt
     2941 acgctggaga tcgtgggcgc gctgcgcacc tgcggggtct ttcccccccg gctgccgcgt
     3001 gatttctggc ggcggctgga ggtcacctcg gcgggcgagc ttaccatggt ggatggcaac
     3061 ctgtggctgg cgcgcgagcg tggcgcgccg cgctggctgc agcgggggct ggaacgggcc
     3121 gccaacctgg ggcggctcga aatcgtccgc caccagcgcg gcgatgtccg gtttctgaag
     3181 gacctgctcc ggctcggcgt ggaacgaacc tacgggcgga tccggctgca tttttaccgc
     3241 cagcgcctgc gggcgttcaa ggcccggaac gcgcccgggc agcgcggccg ctgagcgcgc
     3301 cggcggcccg gggcgccccg acgcccgggg ctgagcgttt aagcaaccgg ccggggcggc
     3361 gggcggtgcc gcgggcggcg gatccgaaag cgcctttacg atgccggccg ggtggagtag
     3421 tggtattctt gatgaaagga agcctgtcat gggcggcggc ggcggagtgg gcggcccccc
     3481 ggggaaggaa ggttcgcccg tccggcagag cgatggctgc cgcggggcag ggaaggaaac
     3541 tatgagcatt atattgaagc gggcctatga cccgccggca aaaaccgacg gccagcgcat
     3601 tttggtggac cggctctggc cccgcggact gaccaaggcc aaggcgaaga ttgatcgctg
     3661 gctgaaagac ctcgcgccct ccaccgagct gcgccagtgg ttcgggcatg acccggagaa
     3721 atgggcggag ttccagaaac ggtatcgcgc ggaattgaag gccaatcccg ccctgtccga
     3781 gctgcgggcc ctggcccggc agggggatat cgcccttatc tacgcggccc gggatcagca
     3841 gcacaacgag gcggtggtgc tcaagcaaga gctggagcgc cgcgcctgac cgcgccccgc
     3901 acgcgccccg cccgcaaaac gcttgccttc cggcggcgcg cgggatagac tcgttccatg
     3961 actggcgggg ctgaaaacct cggccagccg ggtaacggcg cggtgagtca gcccgcgatg
     4021 aacgtgggat gccgatctgg cggccggcgg ccggcgccgg ccttaatgcc accgaccggc
     4081 gtggttcaaa gcccttttcc ggacggacat taaacaacaa acaatactgc tatgaatggt
     4141 tactacgaat taaagacaac gacgacgggc aaacccatgt tcaacctcaa ggccggaaac
     4201 catgaggtca tcctgaccag cgaggtttat gaatccaagg cggcggcgct caacggcatt
     4261 gagtcggtgc ggaaaaacgg ggtgaacgcg gcggccttcg agaagaagat gtccgtcaag
     4321 aacgagcctt actttgtgct caaggccgcc aatggccagg tgatcggcaa gagcgagatg
     4381 tactcctccg aggcggcgtg cgacaacggg atcgcctcgg tgatgcgcaa ctgtccgtcg
     4441 gagaagatcg tcgaagtggc ggcttagtcc gcctatcccg gccgcggggc ggccgccacg
     4501 gcggcgcccc ggaggacagg cgcggcccgc cgggccgcgg cgaaagtgac gggttggacc
     4561 ccggcccccg gggtctgccc gcccccgggc gcgggggcgg gaggtagtgg aggtgtaatc
     4621 atctatgtat caggatatga tgatttatca tttgcgcgcg gcgccgcgag cccttagatt
     4681 gcggcggttg cccgttagcg gaggcagccg gattgcggag cacacttatg gcggatgagc
     4741 agaacagaac ggcgcgggcg caggagctga agcggcaatt gcgcgagcgg atcgtgctgc
     4801 tggacggcgc gatgggcacg atgatccagg cgcatcacct ggacgaggcg gcatttcgcg
     4861 gggagcggtt ccgggattgg ccccgggatt tgaagggcaa taacgacctg ctgaacctga
     4921 cgcagccgcg gatcattcgc gccattcacc gccagtacct ggaggcgggg gcggacatta
     4981 tcgaggccaa cacgttcaac tccacggcga tttcactggc ggattacggg ctggaggagc
     5041 tggcgtatga gctgaatgtg gcgggggggc ggctggcgcg cgcggaggcg gacgcggtga
     5101 tggccgcgca ccccgggcgc gtgtgctggg tcgccggcgc gctcgggccc accaacaaga
     5161 cggcctcgct gtcgccggac gtgcacaacc cggcgttccg cgccatcacc tttgatcaac
     5221 tggtcgcggc ctacaccgag caggcgcgcg gcctgctggc gggcggggtg gacctgctgc
     5281 tgctggagac ggtgtttgat tcgctgaact ccaaggcggc gctgttcgcg atcgcgcagc
     5341 tcttcgagga gagcggccag cgggtgccgg tgatggtttc ctttaccatt accgacttga
     5401 gcgggcgcac cctgtcgggg cagacggtgg aggcgtattg gaactccatt gcgcagcacg
     5461 agctgttgag cgtgggcatc aactgcgcgc tggggccccg ggagatgcgg cccttcattg
     5521 aagagctggc gcggatcgcg aacatctgcg tcagcgccta tccgaacgcg gggctgcccg
     5581 acccgatgca gccgacgggg tttcccgaga cgcccgagac gctggcgccg cagctccggg
     5641 aatgggcggc gaacgggtgg ctgaacctgg tcggcggctg ctgcggcacg acgcccgcgc
     5701 acatccgggc gctggccgag gcggtgcgcg gcctgccgcc gcgggtgccg ccgccgatcg
     5761 cgccggccac ccgcctgagc gggctggagg cgctgaccat ccgcccggag gcgaatttcg
     5821 tcaacatcgg cgagcggacc aacgtgactg gctcgccccg gtttgccaag ctgatcctgg
     5881 ccgggaatta cgaggcggcg gtggccatcg cccggcagca ggttgaaaac ggcgcgcaga
     5941 tcattgatgt gaacatggac gagggcatgc tggattccga gacggcgatg acgacgtttt
     6001 tgaacctggt ggccgccgag ccggacattg cccgcgtgcc gatcatgatt gacagctcga
     6061 agtggtcggt catcgaggcc gggctgaagt gcctgcaggg caaggggatc gtgaactcga
     6121 tcagcctcaa ggagggcgag gcgaacctga tagggcaggc gaagcggatc cggcgctacg
     6181 gggcggcggt ggtggtgatg gcgtttgatg agcgcggcca ggcggatacg ttcgcgcgca
     6241 agatcgaggt gtgcgagcgc tcgtaccgcc tcctgacgca gcaggcgggg tttccgccgg
     6301 aggatatcat cttcgacccg aacgtgctca ccctgggcac cggcatcgag gagcataaca
     6361 actatggcgt ggacttcatc cgcgcgaccc ggtggatcaa ggaacacctg ccgctggccc
     6421 gcgtgagcgg cggcatcagc aacatttcct tctcgttccg cggcaataac gcggtgcgcg
     6481 aggcgattca ttcggccttc ctgtaccacg ccatccgcgc ggggctggac atgggcatcg
     6541 tgaaccccgg cctgctggag gtgtatgagg agattcccca gccgctccgg gagctggtgg
     6601 aggacttgat cctgaaccgc cggccggatg ccaccgagcg cctggtcagg ttcgccgagg
     6661 gcgtcaagcc gaaggacgaa accgccgcgg ccgaggcgga atggcgccag gggacggtcg
     6721 agcaacgcct cagccacgcc ctcatcaaag gcatcaccga tttcattgac gcggacaccg
     6781 aggaagcccg gcggaagtat gggcggccgt tgcaggtcat cgagggcccg ctcatgcagg
     6841 ggatgaatgt cgtcggcgac ctgttcggct cggggcggat gttcctgccc caggtcgtcc
     6901 ggagcgcgcg cgtgatgaag aaggccgtcg cgtacctgct gccgttcatg gaggcggaaa
     6961 aggcggcggc gggggcgcgc ccggcccagg ggcgcatcct gacggccacg gtcaagggcg
     7021 acgtccacga catcggcaag aacattgtgg gcgtggtgct gggctgcaac aactacgagg
     7081 tgattgacct gggggtgatg gtgccggcgg aaaaaatcct gcagtccgcc cgggagctga
     7141 aggccgacct gatcggcctg agcgggttga tcacgccctc gctagacgag atggctcatg
     7201 tggcgaagga aatggagcgc ctgggcttcc gcctgcccct gttgatcggc ggcgccacca
     7261 ccagcaagat tcacacggcg gtgaaaatcg cgccggccta cacggggccg gtggtccacg
     7321 tgctggatgc ctcgcgcgcc gtgagcgtgg tgaacagcct cctcgacgcc cgccttaaac
     7381 ccgcctacct cgagggcatc caggccgatt acgacaagct ccgcgcccag cacgccgggc
     7441 agatgaccaa gccgcttctc agcctcgacg aggcccgccg ccgccgccac ccgctggagt
     7501 ggcaggccgg cgacctcctc cggcccgcct ttaccggctg gcgcgtcctg gcctccgatc
     7561 ccgcggcgcg cggcccgcaa ccggtctcgc ccatcgccct ggcggacctg gttccgttca
     7621 ttgactggtc gcccttcttc cacacctggg aactgcgcgg ccgctacccg gagatcctgc
     7681 agaatcccga ggcgaagaag ctgtttgacg atgcgcaggc cttgctgcgg gagattctcc
     7741 gccgccccgc gctggtcgcg cgcggcgtgt atggcttttt cccggccaat gccgttggcg
     7801 acgacgtcga gctttacacc gacgactccc ggacggaggt gctggccacg ttccatttcc
     7861 tgcggcagca gatggacaaa cccgccagcc agttcaacca ttgcctggcc gattatattg
     7921 cgcccaggcc cccggagcgc ggcgaaccgc tcccggacta cctgggcgcc ttcgcggtca
     7981 gcgccgggtt cggcaccgaa gcgttgtgcc aggaattcga acgcgcgcag gacgattaca
     8041 attccatcat gaccaaggcg ctggcggacc gcctggcgga ggcgtttgcc gagttcctcc
     8101 accagcgggt ccggcaggaa tggggctacg gccgggacga gaacctgagc tacgccgatc
     8161 tgattcgcga gcgctaccgc ggcatccggc ccgccgccgg ctatcccgcc tgcccggatc
     8221 acaccgagaa gcagacgctc tggaagctgc tcgatgtgga gaagaacacc ggcatcaaac
     8281 tgacggaaag cggcgccatg tggccgggcg cgagcgtcag cgggctttac ttcgcccacc
     8341 cggaatccaa gtacttcggc gtgggcaagc tgggccgcga ccaggtgctc gattatcacc
     8401 ggcgcaagca aatggagctg gccgccgtcg agcgctggct gggcccctac ctgaactacg
     8461 acccggccgc cttcggagcg gcccgtccgt aataatcccc cggggcggag gcttgtcttt
     8521 tccgatttgc gtcctagact aggtacatga agaacgatgt gcttccggtg cagattcgcg
     8581 gcatcctgcc cgccaacagc gggtgcgctc tgttcgtggg caatgaggaa aaagtcttcg
     8641 tcatcaacgt cgagccgaac atgggcgccg tcatcgggat gtttctccgg cagacgccca
     8701 aggagcggcc cctgacccac gacctcattc aaagcatctt caagggcttc agcatcaacg
     8761 tcgagcgcgt cgtcatcacc gagatgaaga actcgaccta ctttgcccgg ctgatcctgc
     8821 agcagcagaa cgagctgggc cgcaagatcg tcgagattga cgcccggccc agcgattgcc
     8881 tggcgctcgc caccgcccag aagcgcccca tcttcgtcac ccactcgctc ttcgagcagg
     8941 tcgaggacat gagcgaggtg ctcgaacgca tcaacgagaa cgacagcgag tccgaatagg
     9001 cccatgccgc cgccggctgc ccaacccgcc gcgtccctca agctggtctg cggcgaggat
     9061 gattttgtcg tcaagcagcg cgcccggcaa atccaccagc aatggacggc ggaagtgggg
     9121 gggatggacc acgaaacgat agatgcgagc gtcaccacga aagacgaagc cttccgggcg
     9181 ctggccaaac tccgggaggc cctgcagacc ctgccgttct tcggctccgg gaaggtcatc
     9241 tggttcaagg actgcaattt cctgagcgac gagcgcgcgg ccacgccgga cgtgacggaa
     9301 gcgctggcgg aactggcccg ggaactcaaa gagtttgcgt gggcaaacgt ccgcctgctc
     9361 atcagcgccg gcaaggtgga caagcgcaag gctttctaca agaccctcga caagctgggc
     9421 gcggtcgaga gctttgccgg gtggtcggcg gacgaccccg actgggccga gcaggcggag
     9481 gcgtgggccc gccaggccct ccgggcgcgg cagaaggaga tttccgatga ggccctggcc
     9541 gaactggtca gccgcgtcgg cccccattcc ggcctgctgg acagcgaaat cgaaaaggtg
     9601 tcgctctatg tcggggaacg gcgggaaatc gggcgggcgg acgtggcggc catctgcacg
     9661 cgcaacaaga cggcgcgcgc gtttgcgctg ggcgacgcgc tgggcgaccg cgacctgccc
     9721 cgcctgctgg cccggctcga cgaggcgttg tgggaagtga agctggaccg gcagaagacg
     9781 gagattggcc tgctctacgg gctgatttac aaggtgcgcg ccatgctcct gctcaaggag
     9841 ttgctccggg aaggctggat ccagccggac gcgaatttca gccagttcaa gaagcagctt
     9901 gagcggatcc cggccggcca gttgcccgag gacaaacggt tcaatccgcg cagcctgcat
     9961 ccctttatgc tcttcaaggc gttgccccag gtgaaacgct accgcccggc ggagctggtg
    10021 cgcgccatgg acctcctgtt cgaatgcaac ctgcgcctcg tctccagcgg gctggaggaa
    10081 tctttggtgc tgcagcaggc cctggtgcaa atcgtcagcc ccccggcttc gccgccccgc
    10141 ccggaggcgg cccgggcgcg ccccgccgcg gtcgccggcg cccggcgctg acccgccccg
    10201 gctcccgggc ccggctcact catcccgttc aaacgacggc agctcccact ccttgagcag
    10261 cttccgcgga atctcctgga ggaaccgcga aggccattgc gccaggcccc cgccgctgcc
    10321 ataggcggcg cgctggcggg ggtagcacag gtaaagctca ttccgcgccc gcgtgatggc
    10381 cacatacatc aggcggcgct cctcttcttc gcccgcgggc gacgccagcg cccgctccag
    10441 gggaaacagc ccgtcgcaga gcatgatgac gaacaccaca tcaaactcaa gccccttggc
    10501 ctggtgaatc gtcgagagcc gcagccggtc ctcatccgcc cgggctggcg attcgctctc
    10561 cgcctccagg ttcgtcaaca gcgccaactg ggtcaggaaa tccgtggtgg tgggaaactg
    10621 gcgcgcgaaa atcgccagtt gctccagttc ctccagccgc gtttcggggt tcgtgtaatt
    10681 gctggccagg tattccgcgt aacccgcttt catgaccagg ccaatcatcg ccgccacgtc
    10741 gtcccggacg tcttccgccg ccagttgctc gagggttgcc gcgagctgcg cccaggccgc
    10801 cgccgctttc ttcggcacga cccggccgca ggcccgcagg cgggccgcca ggggcaggga
    10861 aggctccggc ggcgggatgc cgggggcgtc cggcccgtcc gccggaggag ccgccgggcg
    10921 ggcggggggc ggggtgggcg cgccggcggc gaatgcccgc cacagcttgt ccgccccctt
    10981 ggcgccaatg cccggcagcg ccagcgccag ccgcttgaag gaaagctcgt cacgcgggtt
    11041 ggccaccagc ttcacgtaag cgatcacgtc cttgatatgc gcctgctcga aaaagcggat
    11101 gccgctggta atgatgaacg gaatgtcgcc gtgcgtcagc tcaagctgca gctccagcgc
    11161 atgaaaatgc gcgcgataca gcaccgccat ccggctcagg cgcccccctt ccgcccgcaa
    11221 ctgcgccacc cgctgcgcca caaaagctcc ctgctccgtt gcgtccgcgc aagcgaccac
    11281 ccacggtttg cctcccgccg ggcgcgccgg ggccagtttc ttggcgaact ggtgcacatt
    11341 ggcggcaatc gccgcgttgg ccacgcgcag aatctccggg gtgctgcggt agttggtttc
    11401 aatcttataa accttcgccg ccggataccg ctccgggaac ttcaggatgt tcaggcaact
    11461 cgccccgcgc cagccataga tgctttgtgc gtcgtcgccc acggccatca cgttctggtt
    11521 ccgggcggcc agcagatcaa tcagctcgcc ctgcagcggg ttggtatcct ggtattcatc
    11581 caccaggatg aactggaatc gccgctggta gtgttcgccg atctccgggt gctcccgcat
    11641 gagccgcagc cactgcgcca gcaggtcatc aaagtccagc gcattgatcg cccgcttgcg
    11701 cgccgcatag cgctgctgca ggtcccggat ctgcggctcc agccgcgcga agcgggcgtg
    11761 ttggtcgcgc agcaccgcgc tcagcggctg gcgcgtgttc accgccagcg aaaagaggtc
    11821 ggccagcacc tcggccttgg ggaactttcc gcccttggca ttcacctccg cctccgtcat
    11881 gcaggtggtg atcaggcggc gggcgtcctc gctgtccagg atggtgaagt cccgcgaata
    11941 gccaagctga tcggcatgcc ggcgcagcac ccggttgccc agggcgtgaa aagtcccgcc
    12001 ccataatgcc gccagccccc gccccagcaa atccgccgtc cgccgcatca tttcctgcgc
    12061 ggccttgttg gtgaacgtaa gcagcaggat gcggttcggc ggaatgccct gctccagcag
    12121 gtaggccacg cggtagatga gcgcgcgcgt tttgccggaa cccgcccccg caatcaccag
    12181 ggacggcccc ggcggcgccg tcaccgcggc atattgctgc gcgttcagct cccgcgcata
    12241 gtcaatctgc agcgggaccg gcgccccgaa cgggggcaga gcaggctcac gcgacatgcg
    12301 ccgggcggga agctggagcg cgcaatcgca cgggcgcggc ctagcgctgc cacggcgcga
    12361 tctgttcgcc ggcccagatg gctttcaccg gctggcgcgc gcgcaccagc gcggcttggc
    12421 ggcccttgac catgacctcc gcggccaggc tgcgggtgtt gtaattcgag gccatgaccg
    12481 aagcgtaggc cccggcgctc agcaaggcca ggtaatcccc cgcctccgct ttgggcagcg
    12541 gccggtcctg gcagaagaaa tcgcccgact cgcaaatcgg ccccaccacg tccgcgcgga
    12601 tctgcgcgcc ggcccggcgc cgcaccggca cgatttcatg gtacgcctca taaaaggccg
    12661 ggcggatgag gtcgttcatg gccgcgtcca caatcaggaa gttcttcctc cccgtgcgct
    12721 tcacgtattc gatccgcgtc accaataggc ccgcgttgcc cgaaataaag cgcccgggtt
    12781 cgagcaggat cttcagcccc agcggctgga gcagcggctg cagccgcgcc gcgtattgtt
    12841 gcggcgtaat catgccccgg gcggcggacg aacgccacca cgcggcgggc ccgctcgcca
    12901 gcgccggctg atagactatc cccagcccgc ccccgatgct gaaaaactcc agcccgtgcc
    12961 gctccttgag ccgcgccacc agcgggcgca ccttgcgcac cgcctgctcg aacggggcca
    13021 cctcggtaat ctgcgagccg atgtgcattt gcagcccgcg caaccacaga tgtttgagcc
    13081 gggccgcccg cgcataaacg ccctcgatct gctcgaaggc aatgccgaac ttgttctcgt
    13141 aagtcccggt ggtgattttc ttatgcgtgc gcgcctccac gttcggattc acgcgcaccg
    13201 ccacgggcgc ccgccgcttc agccgcgccg cgacccggtt gatgcgctcc agttccggtt
    13261 cgctctccgc gttgaaggtg taaatccccc gccgcagggc gaactcgatc tcgctttccg
    13321 tcttgcccac gcccgcaaac gcgcagcggc gcgggtcgcc gcccgccgcg atcacccgct
    13381 ccagctcgcc cccgctgacg atgtcgaacc cgctgcccag gtccgccagc gcgcgcagga
    13441 cggccaggtt ggagttggac ttgacggcaa agcagatcag ggaatccacc ccggccaggg
    13501 cctgggccag ggcctggtag cgctccgtca gggtgcgttg cgaatagacg tacaggggcg
    13561 tgccatattt gcgaatgagc gactccaccg ccactccctc gcagtaaagc cggttgccgg
    13621 aataatgaaa gtgatgcatg tcgaaggagt gtagaacccg ggccgcaaaa cgcaacgggt
    13681 ttttccggct tcccccggga cgccgctacc gcgccgcgag caagtcatcc acgcgcacaa
    13741 aggcgtagcc cttgcccgcc aggtaatcca gcagctcgcc caaccgggcg gcgaacttgt
    13801 ccgtccgccc cggccccgcg ccaatgtgca tcagcagaag aaaaccgttc agcccccgcg
    13861 gatcctgctg ttccttgcgg aggatgctgt tgaatatgat ctccgaggag acgaagttcc
    13921 ggtccgcctc gcccgtataa tccgcgttgg cgcgcgtgcc gggggtgaag ttcaccacgg
    13981 tcagccccag ctccgcggac caatccacaa tctcctggtt gtaatgctcg taggggggca
    14041 gaaaatagcg gacctgcgcg cgcttcaccc cgcagcgctc gatcttcttc aggttgcgct
    14101 ccaggtcggc gcggaactcc gcgcggctga tcagcgtctt ccgggggcca tcccaggggc
    14161 agtagagcac gtgccgatcg gaatgcggcc ccaaataatg cccctccgcc acgagccgcc
    14221 ggatcagcgg ctggaactcg ccccgggtaa ggaacttccc ggtgaagaag aacgacccct
    14281 tcgccttatg cctggccagt tcatcgagaa tctgcggccc gccctcggca tacttgtgcc
    14341 ccgtaaatac cagggccagc cgccgggccg acgtcggccc ccggataatg ccgccctcgc
    14401 gcaactccac gcccggcccc gccggcgccg gaacggccgc caccacgccg gatgccggct
    14461 gcgcccgggc gctggaaaaa ttcaacgctc ccgctcccgc cagcgccagc aacccggccg
    14521 ctaatctgca atgtttgttc accacccgcc cagcatcaac ctaaaaccgc ccgccaggca
    14581 agtccggctt gcgcccggcg cggcgggggc ggctcaggag ccgggcgcgc gcaggggatg
    14641 gtttcggttg gccagcggga acgcgacccg ttggcgggcc aggccggcgg caaagaccgc
    14701 catcgccatc tccaacgcct tcatgccggc ataaccgctg caaagcggct cccgccgatc
    14761 cgcgatggcc gccagccaat cgtccaccac gcgctgattc gcggccgcca tgctccgttg
    14821 ctcgcgggac agcccccgcg tgggatcctg ctcccaaacc cgccattcgc cggcctggcc
    14881 ctgggccgtc cagctcccgc ctttccgcac ataaatccgc ggcaccatgt ccatgagaat
    14941 cttcacggcg ccccgggcgc cgatgagttc caggccccac ggctcggcca gttcccggtt
    15001 ttggatgcgg ctggtgaagg tggcgttgac gccttgcggg aaagcgaatt gcgcgcagag
    15061 atcatcgccc gccaccggcc cgatgccctc cgtggccggg tgggcgtcgg cgggggtgat
    15121 ctcgcgcccg ccctgccgca cgcgcgccgc gcaccagagc ggatcgcccg caaagaaccg
    15181 cagcaggtca aacaaatgca cccccagcac aaccaaatcc tcgcccccgg cgcggtggtc
    15241 ctgcttgccc tgcgcgcgca tttccagcaa ctcgcccatc agcccgtcgg caaggcattg
    15301 tttgaggaaa agaatattgg gcgccagccg cacctgctgg gccaccacga tcctgagccc
    15361 gccgcgcgcc gccgccgcca gcaactcatc cgcctcggcc agggtttgcg tgatgggttt
    15421 ctccaaatac acatgcgccc cggcgctcaa cgccgcccgc gccatcgcgt gatgctcgcc
    15481 ggtccagcgc ggcgcaacca ccaccaggtg cggcttttcg cgctcgagca tttggcggta
    15541 gtcggcatac gaccgccggg ctcccgcccg cgccgccgcc tggcggcgac ccggttcatc
    15601 cgggtcggcg accgctacca cggtgatatt cggccgcccc gtgaaaatca gctcatggcc
    15661 atgcccatag ttcccgcggc ccgtgtgccc gataatggcc gcgcggaatg ggggagcctc
    15721 cgcgccgcgc gcccgccggg ccaggcccgg cagggacatt cccgcggcga atcccagcgt
    15781 ccgggtcaga aactgccggc gggccagcaa tcccggagcc ccggcagagc ctccaaccgc
    15841 cccgctctgt tcggtcccgg ccatgccgcg cccctacggc gcccgttcaa tcctgagggt
    15901 atgcgtcacc ggcacgaaag cggcccggat ggcggccgcc agcggttcgc gctcccgctc
    15961 ggtttgggcg gcgacggctt ccatatcggc cttcgcttcg gggctgcgaa agcgcgtctt
    16021 gatctgggtg gtttcgtacg cctgtttggc ggccacggcg gcatccaccc gggcaaaggc
    16081 ttcgctaaag ggattgcggg cgaattccgc cgccaggtta accccgcgcc gcaactgctc
    16141 ccccgtgaac gtgcgggctt ccgcgcccca ggtgacccgg tagctcctgc cgctccggac
    16201 attccgcacc ttgagcgtca gccggttcag ctcctcgtta aacggaacca gcttcagcgc
    16261 cgaccggatg ctgctgtccg tggcggggtc gtccgcgccg cagacgggat acgacgcccc
    16321 gcccaggccg gccggcgcgc aggcgcagaa cgggtagcgc gagctttgaa tcacaaactc
    16381 gccctcccgg gcgctcacca gccggtgccc cggggagagc gtcatgcggt tcttcttcaa
    16441 ctcgacctca aaccggccga tgtcgcccgt aagccccatg cctttcaaaa aggcgtacgc
    16501 catcaccagg tggccggccc agccgggatg cacgccatcc tccccggcca gggcgtagcc
    16561 ggggccgtaa acctgccgcg cggtggcgtc ggcggtcagc atggcccaga agacatcggc
    16621 aaacccaacg cgctccgcct ttgcaatctc gatgccgaga ttgcggaggc gcccgagatt
    16681 ccgattcaag tccaaccggg tgtcgttggt atgccgggtc cagcccggca ccttgcccac
    16741 gatgccgggc gccccctgaa tcacccgcac cccgcgggct ttgaacgccc gcacaatggc
    16801 ggtggaagcc tcgcgatagg tctggccgat gcggtcttcg taagcgcgat actcatgatc
    16861 gttcatgccg tagcaagtgg tggccagggt cggccggaac cgcaggcaat cgttggtcat
    16921 gcgcgccagg aaccccggcg cccgctcgcc gccccagcca tattgccgca ccgacacgtt
    16981 caactccggc acgcagaccg tcaggtaatc ctcgatgatg cgcgaataca ttttctgctc
    17041 cgtaatggaa tcgccgcaaa tggcaagccg gtcgccgggc cgcaggagca gccgtttcgg
    17101 cgcgggcgcc ttcgccggct ggtaaagctg ccgcaacgcc tccgccgtct gcggcgctga
    17161 cgcctgggcc aggccggcca ggccggcaat aagcgtaaag agaagaacct gtttcgcgcg
    17221 cataagcttg atgacgccga ttgtttaacc ccggccggcc ggcgtcaagg ttcgagtctc
    17281 cgcgggcggt cgaaccggcg cgcctccttt cccaaccgtt cccccaacgg agaaaccccg
    17341 gcgatgggcg gggccgttca aaacaggaat cagacgagga gatcaggcag caacccgatg
    17401 aagagatgca gccggtgcaa ccgccgggtc ggagaaatgg cttccacgtt ccgaaggcat
    17461 agtttcggga aatacgccat ccccgcgcgg cgcggcgcgg ccgcgcttcc gccgccgccg
    17521 ccatccggcc attccgccgc cgctcctcct ccgggttccc ggcggctacg gcgccgggtc
    17581 ggggaggccg cggcggggcc gcccacgcat cgccggtgaa gacgccggcc aggagggcgc
    17641 caactttcgg aaccggcgcg aggccacgct ttgcgtccgg ccgttttcct gcgtgagaat
    17701 cagcgccgcc gcgcgcggcc aggagtccac caacgccagg ccgcgtttga cgccgagcag
    17761 gctcaccgcc gtatccaggc tgtccgaggt cgtggcgtcc ggcccgatca cggtcgtttg
    17821 tatccggcgc gtcaggccga agccggtggc cgggtccaca atgtgcgagt agcggacgcc
    17881 gtcaatgacg atgaactgct ccgcatcgcc ggcggtggaa acacccgcgt tgcgcagccg
    17941 gagcggcgcc gggaggccgt tggtgtcgcc cgcgaacccg gccagcgcaa cccgccagcc
    18001 gcgccgcccc ggcggcgggt cgccgatggc cagatcgccg ctggccgcga cgagggcgcg
    18061 gttaatgccc ctttccttca ggagccgcag ggcctggtcg gcggcgtagc ctttgccgat
    18121 gctgcccagg tccagccgca ttcccggcac gcgcaaggtc accgtccgcg cgcgcggatc
    18181 cagcgcgagc ttccgccagc cgaccgccgc ccgcgcggcc gcaatctccg ccgccgtggg
    18241 aagcacgccc cgcttgcggg agaaccgcca caggcggaca aaggggccga tggtggggtc
    18301 aaaggcgccg tccgagagcc gggccacctc gcaggcgcgc tggagcacgt caaaaagctc
    18361 cgcgctgacc ggcaccgggg tgccgctcgg gtgatcccgc aggcggttca actcgctgtc
    18421 cgcctggtaa tcgctgagga tgtcctccag ggcgtctatg cgctgaaacg cggcgcccgc
    18481 cgccgcccgc gccgcgccgg gatcgggcgc atagagggtg atgcgcatga gggtgcccat
    18541 cgccgggtgc gtgaactcat agcgctgcaa cggcttgccg cgcggcgtcg aacagcaacc
    18601 cgccagcagc aggagcgcgg cgccccagcc gcccgcccgc cacccccagc ccgaacgccg
    18661 ggagacgccc ccacccccgg gcgccggcgc ggaaacggcg gaaaacttca tacgcgaccc
    18721 ggcccaacaa aaggccggcc gacccggccg gcccgaaacc ccggcgctgc ccggcggccg
    18781 atcagatctg gacggtgggc accgacggcg gatccttata gatgtcgtgc agacccggca
    18841 tgggcggctg cagggttcgg aaggcgcccc aggtcatttt cgccggctcc gtatagagca
    18901 ggtcggggcc atagcggaac gtcgagttca gcgccgcctc ccaatccccg gccgcgccgc
    18961 tgtaggccgt ctcgcgcccc atgatcgcgg tcagcgtgct gtccgtcacc tgcttgccct
    19021 cgttcaaatc ggcgtcccgg agaatggcgt tgatgaggtc aatatgctcc tgcacgtagg
    19081 ggctcacctc ggcgcccgtt ttccggtagc gccaggccgc gccgccctgg ggctgaatcc
    19141 gcccgccggg atcggcggtg ccccgggtgc cctgcacggc ttcgctgacg gaagaccacg
    19201 cgcgcttgat ctggccgcag tagctgaaca tccgcgcgcc attcgggtat tcatactcga
    19261 cggcaaagtt atcccaaatc tcgcccgact tgccgtccaa ctgctgccgc gcgcccatcc
    19321 cccagcattt caccgggtga tcgcccacga tccagttgca gacatctata ttatgcacgt
    19381 gctgctcgca aatatggtcg ccgcacagcc agatatagtg ataccagttg cgaatctgct
    19441 tctccagctc ggtggcgccg tggtcgccgc gatgccagat cggcccgccg ttgacccagt
    19501 aggcgcgcag cgtgtggatg tcgccaatcg cgccgtcccg gaggcgctgg accgtctcca
    19561 catagccccc ctggtgacgg cgctgcgtcc cggcgacaac cttcaggttc ttttgccgcg
    19621 ccgccgccgc cgccgcgtac ataatccggc agccggggcc gtccaccgcc acgggtttct
    19681 ccatgaacac atgcttgccc gccgcgacgg ccgcctggaa atacgccggc cggaaaccgg
    19741 gcggcgtggc cagaatggca tactggacat tcggcacctc cagcgccttc tggaagccgt
    19801 caaagccgct gaaacacttg tcctcggaca ggccgaagtt ctccacgccc ttccgggcgg
    19861 cgtcgggaaa gatgtccgcc acgcccgcaa ttttgccctc caccccggcg gccttggccg
    19921 cttccaggaa gtcccggccc gcgccgctgc cgcgcccccc caggccgatg atcacggcgt
    19981 tgatcacccg cgggctctgg gcgtggagca ccgccggaaa gctcgccgcg gccgccgccg
    20041 aggcggccag aaccgtcgag tgcttaagga actggcggcg cgaaaccgcc gtcgaagacg
    20101 aggagagatg atcaggcata ggtcctttca gtttgatgcg ttggtttaac attaaaatct
    20161 caagcgcggg ttccggcggc cccggcgccg ctagtctttt tccacgccgc tggtccaata
    20221 cttctgcatc tgctcgatcg tgggcacctg cagcgggcgc acgaggcgga agccgaccca
    20281 ttgcgcgtcg gagaaatacc agatgctctt gggcaactgc gggtcctgca tcttccaggc
    20341 ccgatccgag ccccgccgcg ccgcgctgcg gcactggtcc gcctcgtcgt cccacgagcc
    20401 gccccgcacc gagtggggat agggctgggt cgccttgttc cacggctcca cgctgacgcc
    20461 cgccgcggcg catttctggt agtagtccgg ggcgtattga tccagcaccc attccaccac
    20521 gttgccgcac atgtcataca gcccccacgg gttgggcttt ttcttgccga ccttctgata
    20581 cttgaaatcg ctgttctccg cgtaccagcc atactcgccc agcttggccg ggtcgtcgcc
    20641 gaagaaataa gcggtggtcg tgcccgcgcg cgcggcgtat tcccactccg cctcggtcgg
    20701 caggcggtaa aactgcccgg tcttggcgct gagccactgg caatacttgt tggcggcatg
    20761 ctgcgtcatg gcgatggccg ggaagccttc cttgcccatg ccaaagctca tctcgacata
    20821 cggccgggag gggtgcgtga cggcgtccgc cagcttgtcc ccggcggcat cggtcgggag
    20881 ggtggcgcgg gtgcgccgct cctcgtccgg atacatgaac agctcatact cattccaggt
    20941 gacctcgcac tggcccatcc agaagggggc gatcttcacc ttgtgctgcg ggccttcgtc
    21001 cggcttgcgc cccgcctcgc cggccgggct gcccatcaca aactcgcccc cggggatcgg
    21061 caccatcaca aagctcacct gcgtccccgg gatggtgttg gtgtacggct tcatcccctc
    21121 cggccccgcc accgccggct gcgcggcgat gcgcgcatga atgtccggga taatgacgtt
    21181 gtcattgtcc gccagggaag ccaggctgcc agccgcgccg ggagcgcagg cgccgccgct
    21241 gaccttgaac atttgatagc gcgtggaaaa cttgcccccc gccggctggg aaaagacgat
    21301 gaacccgccc ggcgccgccg ccgcgcaggt gaacaaatca ttgttggccg gcaggcggtg
    21361 cgccagcgcc ggcgcccgcg tcccgtcaaa atcatacaac ccggccttct cgccctcccc
    21421 aaaaatgacc agcagcttcc gggcgccgcc gccctcgagc gccaccaccc gccgcaccgg
    21481 ctgcccgaga tcaaaactcg cgcccgcgcc aaaccggaac gtcccggccg ccggctcctc
    21541 caccggccgc accgtcacgc ggctctcccc gggcttatag acgatgaact cgcacaacgg
    21601 cgagccgcga aaccgcccca cggtataatc cgacccggcg ggcagccctt ccgccgacgc
    21661 caccaccacc ggcttggcgc cgctcacatc cagcacccgc agcatgtccc cattgtcccc
    21721 ctgcaccaaa ccgcagacca gctcccgcga ccccgccttg agcgccacgc ggttgccgcg
    21781 cacggccgcc ccgggcagcg tctcctccgc cagcttggga aactccgcgc catcattgcg
    21841 cagcagcacg cccaggttcg gatcgggcga attatagatc gagcccacgg ccaggtccgc
    21901 cagctcggtg ttccccgcgc cgcccacatc caccgcgacg atcgtattgg ggcccaaagc
    21961 ggcggtgaac ggcaacgcca cgggcttgct cggcgccgtc gcgctggaga catccaccag
    22021 cgtgatctcg ttggcgtcgg gcgaggtgaa cgccagcgcc tcctggtcct tcttcagcaa
    22081 cggcccgagg ctgaacccgg tgacgtcctt gaggccgctg ggccggcaat ccacccacgt
    22141 aaaaaccccc ggcgccaact ggtagcccag ccggaacttc ccgctgctct tgtccacgat
    22201 caccaggtcg gcgcgcccgt cccggtcaaa gtcgcccccg gcaaaaaact cctgcggggt
    22261 ttcgtaaatc agcgcggggg cggcggattc cgccggaagc ggcagcggcg ccagggacag
    22321 aaacgatagc accgccgcca atgaagcata gtatttcata cgtcaaaacg tggggctcag
    22381 tgttgcgggc ttccggcggg ttgtcaaacc cggaacgcgg caaaattgcc ggccgccccc
    22441 cgcccgcgct ttccccccgc gcccgccggc ccgcccgtca gcggtggaac ttcaacgccc
    22501 gccgcagcgt gggttcgatg gccccggcca tccgataaaa acccaggtcc gtcgcgtgca
    22561 ccccgtccac cgtgccctcc cggtcatcgc ccgtcaaacc cgccccggac acataccaca
    22621 gccgccgatc gcccgcgcgg acccactggc ggtgcagctc gcgcagccgc gcattggacc
    22681 gcgcgcagcg cccggcctgc ggccccagcg attcgctgtc gggataaacc gcgtgctcca
    22741 ccagcacgat gggcgtccgc ggatgcgcct tccgcaggat cgcaatgaac tcccgcagcc
    22801 gcggcgccac ccactcctcc ttcatgttgg ggagcgggtc cagcacgtag gccgcgggat
    22861 ccagctccgc gagcaggcgc gccatctccg gctcgcccca cgcgttgccc gaaaacccga
    22921 ggttgatcgc ctcccggtcc aggcgccggc ccaggatcgc cggatacgcc atgcccggcc
    22981 gcgccgcgca cccgccctgc aaaatggagg tgccatagaa cacgaccggc ttccgccccg
    23041 gctcccgcgc cggcgcggct tccagcttcg ccccggccgg aaggccgatt tgcacctcgg
    23101 tcacgccgtt atacaaaggc aggtaaagca aatactcgcg ccgcatcggc ggcagttccc
    23161 gcgccagcgt cttctcctcg gtcggatact tctccgcgcg cccggcgccc agccagcgcc
    23221 accggccttt atctttgacg taaagatcca ccccgctcac cccgctggcc ggcatgtgcg
    23281 ccatggccag ccgctcgctc cgcagcgtcc accgcgccga aatcgccgtg gcgtccgtga
    23341 caaatcgcac cgccagcccc gcggaatcct ggctcaaccc ccaaaccgcg ccgcgcacga
    23401 tcccctccgc gcgccccggc agccggtcgt aatactgccg ggtatcccgc caccccttcc
    23461 cctccagaac cagcccccgc gcatcatgcc actgccagcc gccgtccggc tccgccgcct
    23521 gccccgaaac catcgccgcc gccagcaaac accccagaag ggcaaccact gccttatcgg
    23581 taatttgacg cataatcgtg ctaaacaaag ccgccgcgaa aataccccgc cgccgcagcc
    23641 aactccagcg ggaatagtag cgcgtgccct tggtggcctg acgcctcgtc ccgaccgctt
    23701 cccgaacccg cacccgccgc agttcgtcca gtttctcatt caagaacacc gccttgaacc
    23761 caatgggcac cgtctggatg cgccgaaagc gcttgccctt gcggatgatc tcccgccctt
    23821 ggcaatgggg acaaaccatc aaacaagggg ccaccgtcag gtgaaacccg gccgttccgc
    23881 gacgatattc ggtgcggaca tactcgtaac cttcccgcac cccaaaggca tgataaagca
    23941 gactttggct cacccaccca gactaccctc cccgccccaa aaacaaaccc gccggatgaa
    24001 cccgaaaaac caggacgcgt ggcaacaccc aaccgcccgt tccggggttg ccgaacgttc
    24061 acgtccactt cctcccgcct tgaaacgtcc ttttacatca tccgcaagtc gtccatccct
    24121 ccattccggc tcaggcccgc gcccaaaccc gccccacccg ccccagccag cctaacgct
//
LOCUS       DAOPQG010000055        23819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2667_length_23819_cov_14.989859, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000055 DAOPQG010000000
VERSION     DAOPQG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 23819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23819
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2667_length_23819_cov_14.989859"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            492..956
                     /locus_tag="PKI07_07900"
     CDS             492..956
                     /locus_tag="PKI07_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51015.1"
                     /translation="MELNETTQPTYYTARYRHGVDDKRRVQIPAKWRPAESGTELTLM
                     LWPKAKEGPCLRVLPPQQMAKLVRDINAMPNDNPNKVVLKRFIGGESVQVTLDKAGRI
                     CLPEEMARAAGIKDEVVLIGLLDRFEIWSPDRCAKVRAADSIMASEAFKIME"
     gene            964..1494
                     /locus_tag="PKI07_07905"
     CDS             964..1494
                     /locus_tag="PKI07_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51016.1"
                     /translation="MSLLRLLTAGKSLVSVRDTGSRYRLTTQRLLPQFAPARNPFAGK
                     PAEAEPPPAPAVSPETGAARVSSASGARGADSVREQKAARWWNGSKRIVSGLMARPRR
                     VFAKPARSPRGEPPVQGELSLDRVKVVRNDLSETDVEVTTVKPKAPPVPLTLAAAGGV
                     KSGWSAAARFFHAGKR"
     gene            1515..2483
                     /gene="rsmH"
                     /locus_tag="PKI07_07910"
     CDS             1515..2483
                     /gene="rsmH"
                     /locus_tag="PKI07_07910"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOC51017.1"
                     /translation="MPEYTHNPVMVAEVLAALRPAPGGRYVDGTIGGAGHAAAILAAS
                     SPTGWLWGCDRDGAAIEAARSRLAGFAGRFELRQANFAELAEWVPPESCDGVLLDLGI
                     SSPQLDEAGRGFSFQLDGPLDMRMDQRQAETAARWVNEAGVEELARLFWELGGERRAR
                     QLARVIARERERRRFATTRQLADLIERWAPRGGRKAHPATRVFQALRMAVNDEIGSLQ
                     QGLAKALRVLKPGGRLVVITFHSLEDRLVKEFGRARARDYSFPGTVDVPALRAPRAPE
                     LRWVNRKAIRTSAAEAAANPRSRSAQLRVLEKLGGTNDADLEQNSR"
     gene            2485..2871
                     /locus_tag="PKI07_07915"
     CDS             2485..2871
                     /locus_tag="PKI07_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51018.1"
                     /translation="MARNRKYQSAAIRFGPALKAFLLCLLLGGSGLGYVWLKDQISRL
                     GRQIKQSELRLDELQYQSDKLRKQLGAMRSPLFLERRIQELGLGLAPAQPAQVWLLAE
                     PAGDEPHGGSARQYAAPRLGQGEPNG"
     gene            3099..5012
                     /locus_tag="PKI07_07920"
     CDS             3099..5012
                     /locus_tag="PKI07_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOC51019.1"
                     /translation="MPGTLQSQRLVMLTLLMGAAFAALAYRLIDLQVLRYDELSAKAR
                     GNTIREFLLEPRRGDILDAKGNLLATSVFVKTVCADPMLIGPRAAEVARAVAPLLQMS
                     EAELQERLKPRLTRNEQGVLITNRYVVLKRKVQLETWQQIQAVMTNLSFGLDEKKLSR
                     AERAFYRGLRRAGVYAESVDDQLRVYPNHALAAHVLGYVGMSEREIDGRRHLETSGKD
                     GLERVFDSKLAGVRGWRVTETDRPGREVVSLRKQNVEPHDGYNLVLTLDSVAQHIVES
                     ALAEAMEKYSPVSISGLVVRPRTGGILAMATLPNFDPNNPGAAVPDARRNRIITDVTE
                     PGSTFKIVVVSGALNDRVVQLSDSFDCENGRFHFAGRVLHDHKPYGVLSVENIIAKSS
                     NIGAAKIGIKLKEDRLYDYIRDFGFGARTSLSLPGEVSGIVHPVKNWSKVSIVQIPMG
                     HGMAATGLQMTMAMCALANKGVLMRPMLVDRLEDRDHNVITKYPPQRVRQVISEAAAR
                     QMIQALKAVVLPGGTAPEAALEHYTVAGKTGTAQKSGGPSGYLPGKYFASFIGFFPAD
                     EPEVCISIMLDEPKHGYYGGQVAAPVFKQIAERLAGYLNINPEDGETLPGTGAVTVSL
                     DNRTFKTAAARSQ"
     gene            5039..5254
                     /locus_tag="PKI07_07925"
     CDS             5039..5254
                     /locus_tag="PKI07_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51020.1"
                     /translation="MNSLPLIPHHLFVAAIGNDGRPGQKSSRPEPRTVWPAGRTAAGT
                     LPTMDAGEPTPVSMNRRSPEPVNLASL"
     gene            5295..6692
                     /gene="murF"
                     /locus_tag="PKI07_07930"
     CDS             5295..6692
                     /gene="murF"
                     /locus_tag="PKI07_07930"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOC51021.1"
                     /translation="MEPRSLGYVATACAGDLRDGAHEALVHRVCTDSRQAQPGDLFFA
                     LAGERFDGHDYLPQAVAKGVGAVVAERSRLPAGKCGCPVIAVESTRQALGRLAARYRE
                     DFSLPVVAVGGSNGKTTTKELVAAVLRQQFPTLWSEASFNNDIGVPLTLLRLDSSHRA
                     AVLEVGSNHPGELAPLVQIIRPNYGIITSIGREHLEFFGDLAGVAREEGWLAELLPVN
                     GRLLVNGDSEWASLLAKRTRAQAVRVGLGAGNDWRARDLCSDERGIRFLAEGPRADFA
                     GEYRIRLLGRHQVVNALFAIAMGAELGMEREAVAQGLAKCQPLKMRLELWEAGGLSVL
                     DDSYNANADSMEAALQTLQELPCKGRRVAVLGDMAELGAYSSTAHEEAGRRVAELGIG
                     QLFAIGTMAPIMARAAREAGLHRVFEFADVETAAAAVGSFVKSGDLVLLKASRAARLE
                     RIGDRLRNRPAAKGN"
     gene            6693..7841
                     /gene="mraY"
                     /locus_tag="PKI07_07935"
     CDS             6693..7841
                     /gene="mraY"
                     /locus_tag="PKI07_07935"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOC51022.1"
                     /translation="MFYYLYQYLQDWTAGTEWAERLSAFRLFRYITFRSAGAALTALV
                     LSWWLGPKTIAWLKQLKFGQEYQDKAEAGGGIATRQRSKKGTPTMGGILIVIVLNLTT
                     LLWAQWNTQVQLTLLSVVVLAGLGFYDDYAKISEQSSRGTRSGVKLGVQTLLALFIGL
                     YLWRLPSTQRLITEIMVPFFKYPVLTGAGLVGLALTVLTIVGSSNAVNLTDGLDGLAI
                     GCTLIVSFTFLVLTYLAGHRTASAYLQIPHVIGAGELTVFCAALIGAGLGFLWFNCHP
                     AQVFMGDTGSLALGGALGIVAVLIHQPLVLIIAGGVFVLEAISVILQIGWYRYTKRRS
                     GTGARVFLMAPLHHHFEKKGWYESQVVMRFYILGVLCALLALSTLKIR"
     gene            8765..9532
                     /locus_tag="PKI07_07940"
     CDS             8765..9532
                     /locus_tag="PKI07_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOC51023.1"
                     /translation="MNNQNPLILQGSAMEQKNRGRNRVKIAVFFVLAIHGIGLMALLM
                     QGCGQSKEPVPPPEATETNELPPFVEPTPPPPPTNEPPVVEAAPVVPETPPPPPVVPV
                     EVTEYTIVKGDYLERVARNHHISLKALLEANPGIEPTRLKIGQKIRLPAATAPAATVA
                     PQTASTEAAVGSGEKIYTVKSGDTLTHIARHFGTTPKAISAANNLKTTSIKVGQKLKI
                     PTKAAALPATAPSAPITPVETVPMAPAAPPPPTPPET"
     gene            9589..10737
                     /gene="ftsW"
                     /locus_tag="PKI07_07945"
     CDS             9589..10737
                     /gene="ftsW"
                     /locus_tag="PKI07_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOC51024.1"
                     /translation="MKMAVTTLVSSVAALLALGMVMLYSSSMAQVGARYLTMQLAWCA
                     LGLGACLVTALLNYRQLKKIWWLPLLIALVLLALVLDTHFGTRINGARRWFRLWGLSF
                     QPSEFAKIALLIALAWHGDFYRRQVSRWKRGILVPGALIGLTVGLIFLEPDVGNAMVL
                     AAVSGMVLLISGIPLRYFLLPALIAALGVGLFIYYNPMRSGRIYSWLHLEETRRDTGL
                     QAYQSMVALGSGGLTGKGLGDGRQKLGFVPEHHTDFIFSIIGEELGLMVTLAVVAAFV
                     AIIFSGAHIARNAGDIFGMLLASGITFLIGLQAFINIGVVTSALPNKGLPLPFISYGG
                     SSLVIMLGCVGLLLSIARQAGEPDSAMRGGLITNDSANPFARGNQPEP"
     gene            10734..12014
                     /gene="murG"
                     /locus_tag="PKI07_07950"
     CDS             10734..12014
                     /gene="murG"
                     /locus_tag="PKI07_07950"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOC51025.1"
                     /translation="MTPAASLSVQVAIACGGTGGHLFPGLAVADELVRHGCAATLFIS
                     PKEVDQQAVRDVSGMEIITLPAVGWQRGRSGAFLRGFIRSYRATAKLFKTRRPQAVLA
                     MGGFTSMPPILAARRIGARTFIHESNAIPGRANRWLSRVVHGAFIGFPETAGRLRARN
                     LSVTGTPVRPGFEVRDSSACRVQLGLDPERPVLLVMGGSQGARGVNELLIQTLPLLKA
                     DGADWQWLHLAGAGDAQKVVQAYAGYRLKAIVRPFLTEMEVALGAATAAVCRAGASSL
                     AELAALRVPAVLIPYPAATDNHQFFNARAFESSGAAYLLEEKTASPEALRQLLLDLVH
                     KPAVREKMRDALARWHTPCAAEQIASTILAAVEVEAGKRNYHAGQLPAARPGTDRGDA
                     ATARNPSASAETQPMRINHVADYDKTLLPAGRVA"
     gene            12032..12958
                     /gene="murB"
                     /locus_tag="PKI07_07955"
     CDS             12032..12958
                     /gene="murB"
                     /locus_tag="PKI07_07955"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOC51026.1"
                     /translation="MAKKPHSGKEASAELTRRVSAATAIRCDELMARHTTLRVGGPAD
                     LYVEPASEADLVEVVAFCREQALPFFILGRGSNLLVKDGGFRGVVISLAHAHFSWITV
                     EGERLNCGAGARVKMVVAAARQHSLAGLEFLEGIPGSIGGALRMNAGAMGGAIFDRVE
                     TVRFMSPDGQVHERVAQELEPRYRRCPFLQTQIVLSAVLRGHAGSSEAIRQRLNEYSE
                     KRRQSQPAAPSAGCIFKNPGTHSAGQLIDELGLKGTRVGGAVVAAKHGNFIVNSGGAT
                     AHDILALIEIIRRRVQAERAIELEMEVEVIGE"
     gene            12955..13887
                     /locus_tag="PKI07_07960"
     CDS             12955..13887
                     /locus_tag="PKI07_07960"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOC51027.1"
                     /translation="MNSSLTKLNITVMLGGPSAEREVSLRSGAGVVKALRSLGHQVNE
                     LDPQNPDWKLPEGTEVVFLALHGTYGEDGTVQQRLEELGMVYTGSDPEASRIGFDKYL
                     TKQRCVAAGIPTARYLLIESLTASWPMGWDPPVVLKPARQGSSVGLQFVERVSDWSTA
                     LAEAMRHDSRVLLEEKIAGRECTVGILADEPLPVVEVRPKSPIYDYQTKYSVGSTDYF
                     CPAPFDAAMTARIQAAGLGAFKAIGGRDYSRVDIIVQPNGELVVLEVNTLPGMTEISV
                     LPKAAAVAGLSYPQLCQRMVELALQRKHVVQTQT"
     gene            13964..14824
                     /locus_tag="PKI07_07965"
     CDS             13964..14824
                     /locus_tag="PKI07_07965"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOC51028.1"
                     /translation="MAALTLGAVFGTVFGLYLLYRGWDWTLDRLVYKNPAFAIQRTDI
                     ETDGNIALDQLRHWAGIRTGENLLALDLARVKRDLEMVPLIQLVSVERILPHTVCIRI
                     TERKPVAQVNLPGSRPGEATPRAVYHLDASGHVMYPLEPRQRATPANPADEALPVIGG
                     IAPGELQPGRCLNVPQAQAALRLIEAFKESPLAGLVTLKHVEVAAPEVLVVTTGQGSE
                     VTFALTDLKQQLRRWHAIFELGRQTGNTIATLDLAISNNIPLRWFEAAADPSAEPKPV
                     RVTHSRKKNV"
     gene            14817..16040
                     /gene="ftsA"
                     /locus_tag="PKI07_07970"
     CDS             14817..16040
                     /gene="ftsA"
                     /locus_tag="PKI07_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOC51029.1"
                     /translation="MFNRSSIIVGLEIGTSKVCAVVGDLAADDALNIVGLGQARSRGV
                     RKGEIVDVAVAEEDVRCAIVEAEKMADVEIRSVYLGVTGSHLQGFNNRGVYPVASADR
                     EITADDVQDVIRSAKTINLPAENHTLHAIRQHFFVDGQDGIPDPVGMLGSRLEVDVHV
                     VHGNRNRLQNAIRTVKGLQLEVDDIVFNGLAASLALLTSEQKELGSLVIDLGGGTADY
                     AVCANGIIKHTGVLAVGGDHVSNDLAYGLKVSLSRAEQLKIEHGSSFVTPDDKGQSLT
                     LPNEHGLPHKTVNLEHLRRIMSLRLEEIFRLIGQDLEQADVLDYLRAGVFLCGGGAHI
                     PRITQLAEQVFQVPAFLGQTNSISGLKSALNQPEFATAIGLVKFGSFQQRKSGDKSSW
                     ANSFKNTLSQLFRCL"
     gene            16058..17353
                     /locus_tag="PKI07_07975"
     CDS             16058..17353
                     /locus_tag="PKI07_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51030.1"
                     /translation="MITSAHNEAVVTTPHRTLAVQIIGVGGAGITLVELMRQGEPSGA
                     DFAVVDTDARSLAVSGARKKVHLEIPHPRGLGAGNDPDRGRALAEEQFSKLKALFTGA
                     DMVFVLAGLGGGAGTGIGPVVARAAKEAGALTLGFVAMPFDCEGRHRQRLALQGLTQF
                     KAAADGAICLPNQKLCKLIDENTSVLETFKIINEFLVTGVFGVWRLLRHRGVIEIPFA
                     ELTALLQDRHTECALAIAEAIGPTRSREVMDKLLAHPLLDGGQVLVEAETVWVNLTGG
                     PDLSMADINRVMGQINEQCERAQVIMGATVDETFKDRLAITLVAARKHPECDAWTKTA
                     PNFGEPDQPRLSPLTGERSGSRFASVAPVLTPEQVAQLPARQGSGALRQRKSASRMRQ
                     AQLPLEIVSKGRFDKSEPTVHKGEDLDVPTYIRQGVPLN"
     gene            complement(17434..23595)
                     /locus_tag="PKI07_07980"
     CDS             complement(17434..23595)
                     /locus_tag="PKI07_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC51031.1"
                     /translation="MRSRFLIWLLSSSMLLALAACIGPRDKQRASEPAGERGAATEVH
                     LSPAADAVAPALLSALPAAAPAPVLRTPSRAGSSSLRLENTATPLCQLVRRPTALLLE
                     NALLDTAQPLALSIPDHLRASGDPGAYIVQSRAPLDDAFRIRLRAAGAAMIAYIPNQA
                     YLVGAPAAVAQRLQADPQVQAVLPYEPYFKLKPPLLALALERQALPEAQPLHLTLFPD
                     SAAEVLDQLRDLGVEAVGDGPSPFGPVLWVRGRRADSSTGASDLLPALARLPGVQQVE
                     LARARVAANDLGRCEVAAAADAITPVNYLELTGSNVLVGVADFGVDANHPDLAGRVLC
                     DAPASGIDTNGHGTHVAGIIAGSGLQSPTVTQASGSVMPGTNLQFRGQAPAAQVLALT
                     AQAGPASDAYLQQTAARHGAFISNHSWHYAGDPSYDLAAARYDAAVRDALPGEPGAQP
                     LLLVFGAGNAGNGSEDGTGGDADAIQSPATAKNVITVGAVAQARQIAAQAWECGVVNE
                     TNHCQTNSPWLGQTLTNNVMAVFSSRGNVGVGLEGAFGRVKPDIVAPGVFVVSARSTQ
                     WDSGAYYGPPAGSNNYSAVLSNLNETLGPFYRYESGTSLAAARVSGSLALMQEFFLRL
                     GRTNSPALMKALLINGARPLATPGAFSPHMPTNASGWGLINLTNSLPPALSNAFAGVA
                     APMHVFDQDPANALATGQRHTRFLSLSSPATNAPLRITLVWTDPPGNPAASLKLVNDL
                     DLIVTNLDTGEVYLGNDIPPGGSVNQAWNPAQPPNADVVNNVENVYLPPQVGASYSVT
                     VSARAVNVNAVSARTNGVVQDYALVIASGDGAEADALTVTDTPLVSEDSPRVMVVTNS
                     FEPGQGISGGLLLNQRAGAMGPQADDRSIPWAGGASGEIGVGLTNQWRFYVLTNEQNY
                     PHAAFLTFMAADLSPSLPGLFQTNLNPAAPAEADIDLYVSTDSALTNLGSAALAAAHK
                     ALGRGGTHAIVLGNAAPGALYYVGVKAESQLAAEYMFMGVFSQQPFGEPDANGSWLLR
                     GINLPATIPAGAAARPGVANVIAVAPAPVAVRRVVVTNELWQAGFTNLICALRHGSKT
                     AVLHNRSQPPGEPPPHEYTWVYEDNGESDILGAVPTDGPGSLRDFIGEPGRGIWLLNL
                     ANYEPGQTGLVQNLAIRLDPQNAEAGTPFTVPAHAFAGDFLEIPLGATNLTLCLYNDT
                     PEPLPLEVYLRAGGVPSQTVFDRRLLADAATTCWSFTPYTLPPLVPGRYYIGVFNTNA
                     LPQTLRLESSVGVDPGGGEPVISTGPGAVSLPDDALTNACLFLSDTRPIAAIEVALLA
                     TNHSRISDMVFTLISPAGTRVRLFDGRGGDTADELGGIVLRTNIYPTFYSGDYNANRT
                     NLFVGYNPGILTIDYDFYAAPDTLHVYYGQELIYESGLVSDTNLVVIPFGPGVQTNLA
                     TELVIVMNEGNNEDTNTLWQYTATVASWGPGYVVFTEDTNRTQVPIKFARPPYLPEES
                     SWDFYCRPEQSLSALIGERAGGTWQLEMWDSRAGAGLPAPELASWQLRIVFQNTEPVP
                     VELPPGIARANTVPPGQVAPFAVQAPDWATRATNLLVQASGPVNVLFNQNRPPTGTNA
                     GDITLLAEATEGFHTLNMDGLPPLAPGMRYYLGLQNPGSNDVSAALRVDFDLIPLANG
                     VPFNAIHAGNLLPQYFAYDVSSNATAVSFQLLNPEGNLNLVVRRGDALPTLAGFDYGS
                     FCPGTNSEGIFVFPNTAPVALAPGRWHLGVFNADLKNVAYTIRATEYTNAFPNVVRLN
                     NGEPYYGYNSGGEGTANYYHFVVSTNAVRAQFEIIGPVADMTLLARKGLPLPNAGSYD
                     CRSANPGLNDELITLFDFSSPVSLTPGDWFIAAVNASGEPADYAITATEFPAYGLDLL
                     ITECLVDSYNFCLGWTSMRGLHYYVEGKADLDSTNWVVISPTLTASGNAAWYCVEMPS
                     PYHFFRIGEGLVLEDYGAPVAISRIAVEANGVYLEWTASVRDQFQVQWTPSLSPPDWT
                     PFAEVLTSVNGTFEFRDDGSQTGGLSSPRYYRLRRLP"
BASE COUNT         4768 a         6713 c         7792 g         4546 t
ORIGIN      
        1 ccccagtggc ttaccatgac ccgctgcttt ttgcagaact tttaggacac taccggcggc
       61 cgttcatcct gggaggtgaa gcagggctgg cggtcatcgg ggtttgaatc cagcggcgat
      121 catgtccgct ggttcgttta tgtgcttggg gcctgcgctg ggcataaact tttcgcgggt
      181 gcgaaaggac aagtttcctc ggaaggggac gccttgtttt tccgcaacag agtggcagcc
      241 gggcaaaaag aaagggaatg ttcgtgccat tcatgccggt gcgattcaag gacgcgcgaa
      301 caaggaaggc ttgcgatttg ccctgtggtg ggctagcttg aggatagtca cagagtgggt
      361 ttatgaccgt cagggagctg tgaataactt tttaataatt aagtggggta aggtgttgac
      421 aaagtggggc agtgtgtgta aaaaaggagc gtaatggggc agaaatgcag ggatgcactg
      481 ctgttaacaa gatggaactg aacgagacaa cgcagccgac ttattacacc gccagatatc
      541 ggcatggggt ggatgacaag cggcgagtgc agattccggc gaagtggcgg ccggcagaat
      601 ctggcactga gttgactctg atgctttggc caaaagccaa ggagggtccg tgtttgaggg
      661 tgttgccgcc gcagcagatg gcgaagctgg tgcgggacat taatgcgatg cccaatgaca
      721 acccgaacaa ggttgttttg aagcggttca ttggcgggga atccgtgcag gtgaccctcg
      781 acaaagcagg gcgcatctgt ctgccggagg agatggcgag agcagcgggg ataaaagatg
      841 aagtcgtgct gattggattg ctggaccgtt ttgagatctg gagtcccgac cggtgcgcga
      901 aggtgcgggc ggccgacagc atcatggctt cggaagcctt taagataatg gagtgatcgg
      961 tttatgagct tattgcgatt gctgacagcg ggcaaaagct tggtgagcgt gcgggacacc
     1021 gggagccgtt accggttgac gacgcagcga ttgttgccgc agttcgctcc ggcgcggaat
     1081 ccatttgctg gcaagcccgc cgaggcggag ccgcctcccg caccggcggt ttcaccggag
     1141 acgggggccg cgcgcgtgtc atccgcatcg ggggcgcggg gcgcggacag cgtgcgggag
     1201 cagaaggccg cccggtggtg gaacggctcg aagcggatag tgagcgggtt gatggcgcgc
     1261 ccgcgccgtg tcttcgcgaa gccggcccgg tcgccgcgtg gggagccgcc cgtgcagggc
     1321 gagctttcat tggatcgggt caaggtggtg cgaaacgatt tgagcgaaac agacgtggaa
     1381 gtgacgacgg tgaaacccaa ggcgccgccc gtgccgttga cgttggcggc ggcgggcggg
     1441 gtgaagagcg gctggagcgc ggcggcccgg ttttttcacg cgggcaaacg gtaaagcggc
     1501 gaaccggatg aaaagtgccc gaatacacac acaacccggt gatggtggcg gaagtgctgg
     1561 ccgcgttgcg gccggctccg ggcgggcggt acgtggacgg caccatcggt ggagctgggc
     1621 acgcggccgc catcctggcc gcgagttcgc cgacggggtg gctgtggggg tgcgatcgcg
     1681 atggcgcggc gattgaggcg gcgcggtcgc ggttagctgg atttgccgga cggttcgagc
     1741 tgcgccaggc taatttcgcg gaattggcgg agtgggttcc gccggagagt tgtgacgggg
     1801 tgttgctgga tttgggcatc agctcgccgc aactggacga ggccgggcgc ggcttcagct
     1861 ttcagttgga cgggccgctg gacatgcgaa tggaccagcg gcaagccgaa acggcggccc
     1921 ggtgggtgaa cgaagcgggc gtcgaagagc tggcgaggct tttttgggaa ctgggcggag
     1981 agcgaagggc gcggcagttg gcgcgggtga tagcccggga gcgcgagcgg cggcggtttg
     2041 cgacaacgcg gcagttggcg gacttgatcg agcgttgggc tccgcggggc ggccgcaagg
     2101 cgcacccggc cacgcgggtg ttccaggctc tccgcatggc ggtcaacgac gaaataggtt
     2161 ctttgcaaca gggattggcg aaggcgctgc gggttctgaa gccgggcggg cggctggtcg
     2221 taatcacgtt ccactcgctg gaagatcgcc tggtgaagga gttcgggcgc gcgcgggcgc
     2281 gggattacag tttccccgga acggtggatg tcccggcgtt gcgcgccccc cgcgcccccg
     2341 agctgcggtg ggtgaatcgc aaggcaattc gaacgtccgc ggcggaagcc gcggccaacc
     2401 cgcgcagccg cagcgcgcag ttgcgggtgc tggaaaagct gggcggaacg aacgatgcgg
     2461 atttagagca aaactcaaga tgatatggcg cggaatcgaa aatatcagtc ggcggccatc
     2521 cggtttgggc cggcgctgaa agcgtttctg ctttgcctgc tgctgggcgg ctcggggctg
     2581 gggtatgtct ggctgaagga ccagatttcc cggctgggcc ggcaaatcaa gcaatccgag
     2641 cttcgtctgg atgagttgca ataccaaagc gataagctga gaaaacagtt gggcgccatg
     2701 cggtcgccct tgtttctgga acggcgcatt caggaattgg gtttggggct ggcgccggcg
     2761 caacccgcgc aggtttggct gctcgccgaa cccgcggggg atgaaccgca cgggggtagc
     2821 gccaggcaat acgccgcgcc gcgcctggga caaggcgaac ccaacggttg agagcgcgaa
     2881 tgagactttg gccgccgcct gcgacgccgt cggatgagga accgatcgcc gcaggcggac
     2941 ggcagctttg cgggacggga tgccggggcg ccggaagggt ttgagaaata atttgccgcc
     3001 gcccgtgacg ccgtcggatg aggaaccgat cgccgcgggc gcgcggcaac cttgggatga
     3061 tacggatagc gagaatcact agagctggat gagttaagat gccgggaaca ctgcaatcgc
     3121 aacgactggt catgctgacg ctgttgatgg gcgcggcctt tgcggcgctg gcgtaccggc
     3181 tcattgattt gcaggtgctg cgttatgatg agctcagcgc gaaggcccgc ggcaacacga
     3241 tccgcgagtt tctgctggag ccgcgccggg gtgatattct ggacgccaag ggcaacctgc
     3301 tggcgacgag tgtgtttgtg aagacggttt gcgccgatcc gatgttgatc ggcccccggg
     3361 cggcggaggt ggcgcgcgcg gtggcgccac tgctgcaaat gagcgaagcg gagttgcagg
     3421 aacggctgaa accacgcctg acgcgcaacg agcagggcgt cctcatcacc aaccgctacg
     3481 tggtgctaaa acgcaaagtg cagctcgaaa cctggcagca aattcaggcg gtgatgacca
     3541 acctttcctt tgggctggac gagaaaaagc tgtcgcgcgc ggaacgggcg ttttaccgcg
     3601 gcctgcgccg ggccggggtc tatgccgaat cggtggacga tcaactgcgg gtatatccca
     3661 atcatgcgct ggcggcgcat gtgctgggtt atgtgggcat gagtgagcgc gagattgacg
     3721 gccgccggca cctggagacc tccggcaagg acgggctcga gcgggttttc gactcgaaat
     3781 tggccggcgt gcgcggctgg cgggtgacgg agacggaccg gccggggcgg gaagtggttt
     3841 ccctgcgcaa acaaaatgtg gagccgcacg acggatacaa cctggtgctg accctggact
     3901 cggtggcgca acatatcgtc gagtctgccc tggcggaagc gatggaaaaa tactcgccgg
     3961 tgagcatttc cgggctcgtg gtccgcccgc gcaccggggg gattctggcc atggcgactc
     4021 tgcccaactt cgatccgaat aatcccggcg cagccgtgcc ggatgcgcgg cgcaaccgga
     4081 tcatcaccga tgtgaccgaa cccggctcca ccttcaagat tgtggtggtt tcgggagcgc
     4141 tcaacgaccg ggtggtgcag ttgagcgaca gctttgattg cgagaatggg cgttttcact
     4201 tcgcgggacg ggtgcttcac gatcataaac cctatggggt gctgtcggtg gaaaacatta
     4261 tcgccaagtc gtccaatatc ggcgcggcga agatcggcat caagctgaag gaggaccggc
     4321 tgtatgatta cattcgcgat tttggttttg gcgcccgaac cagcctgtcg ttgccgggag
     4381 aggtgagcgg cattgtgcat cccgtgaaga actggagcaa agtttcgatc gtccagattc
     4441 ccatgggcca cgggatggcc gccaccgggc tgcaaatgac aatggccatg tgcgccctgg
     4501 ccaacaaggg ggtgttgatg cggccaatgc tggtggatcg cctggaggat cgcgaccaca
     4561 atgtcataac caaatatccg ccgcaacgcg tccggcaggt cattagcgaa gccgccgcgc
     4621 gccaaatgat ccaggcgctc aaagcggtcg ttttgcccgg cggcacggct ccggaagcgg
     4681 ccctggagca ttacacggta gcgggcaaga ccgggacggc gcaaaagtcg ggcgggccca
     4741 gcggctatct tccgggcaag tatttcgcat cgtttattgg gtttttcccg gccgatgaac
     4801 ctgaggtttg catttccatc atgctggatg aacccaagca cggttattat ggcgggcaag
     4861 tggccgcccc ggtgttcaag caaatcgcgg aacggctggc gggttatctg aatatcaatc
     4921 cggaagacgg tgaaacgctg ccgggcaccg gcgccgtcac cgtgtcgttg gataatcgta
     4981 ctttcaaaac ggcggccgct cgctcccagt gaacgcacct taacaaccaa tggacattat
     5041 gaactcacta cccttgattc cgcaccacct gtttgtggca gccatcggca acgacggccg
     5101 gcccggacaa aaatccagcc gccccgagcc ccgcaccgtt tggccggccg gccggaccgc
     5161 cgcgggcact ctgccaacga tggatgccgg ggaaccaacg cccgtgagca tgaatcgccg
     5221 gtcgccggag ccggttaacc tggcctcgct ttgacgcggg gccggattgc cgccggacag
     5281 aatcagggct tgcgatggag cctcgctcat taggatacgt tgccacggct tgcgccggag
     5341 acctgcggga cggggcgcat gaagcgctgg tgcatcgcgt atgcacggat tcgcgccagg
     5401 ctcaaccggg cgacctgttc ttcgccctgg ccggcgaacg attcgacggg cacgattacc
     5461 tgccgcaagc ggttgccaaa ggagtgggcg cggtcgtggc ggagcgctca cggctgccag
     5521 cgggcaagtg tggctgtccc gtaattgccg tcgagagcac ccgccaggcg ctggggcggc
     5581 tggccgcgcg gtatcgggag gatttttcgc tgccggtggt agccgttggc ggatccaatg
     5641 gaaagaccac cacgaaagaa ctcgtggccg ccgtattgcg gcagcagttc cccacactgt
     5701 ggagcgaggc cagctttaac aatgatattg gagtgccgtt aacgttgctg cgcctggact
     5761 cgtcgcaccg ggcggcggtg ctggaagtcg ggagcaatca tccgggcgaa ctggcgccgc
     5821 tggtgcaaat aatccggcca aactatggca taatcaccag cattggccgc gagcacctgg
     5881 agtttttcgg cgacctggcg ggggtggctc gggaagaagg ctggctggcg gaattattgc
     5941 ccgtgaacgg ccggcttctg gtgaatggag acagcgagtg ggcatcgcta ctggcaaaac
     6001 gcacgcgcgc gcaggcagtg cgggtgggcc tgggtgcggg caatgattgg cgggcgcggg
     6061 acttgtgttc cgatgagcgt gggatacggt ttctggcgga gggaccccgg gcggattttg
     6121 cgggcgaata tcggattcgt ttgctgggcc gccatcaagt tgtgaacgcg ttgtttgcca
     6181 tagcgatggg ggcggaattg gggatggaac gtgaagcggt ggcccagggg ttggcaaaat
     6241 gccaaccgct caaaatgcgt ctggaactat gggaagcggg tggtttgagc gtcctcgacg
     6301 attcgtataa cgcgaacgcg gattcaatgg aggcggcctt gcaaacgttg caagagttgc
     6361 cttgtaaggg acgtcgagta gccgtgctgg gggatatggc tgagttaggg gcttacagtt
     6421 caaccgcgca cgaagaagcc ggccggcggg tggcggaatt gggaataggc caattgtttg
     6481 ccattggcac gatggccccc atcatggcgc gggccgcgcg ggaggccggc ctccatcggg
     6541 tattcgagtt tgccgatgtg gaaaccgcgg cggcggcagt gggcagcttc gtcaagagcg
     6601 gcgatttggt gttgttaaaa gcctctcgag ctgcgcggtt ggaacggatt ggcgatcggt
     6661 tgcgaaatcg ccctgccgcg aaagggaatt gaatgtttta ttatctgtac caatacctgc
     6721 aggactggac ggcaggcact gagtgggctg agcggctctc cgcgtttcgg cttttccgtt
     6781 atattacctt tcgcagcgcg ggggcggcgc tgaccgcgct ggttttgagt tggtggctgg
     6841 ggccgaaaac catcgcctgg ctcaagcagt tgaagtttgg acaggaatat caggacaagg
     6901 ccgaagcggg cggtggaata gccacgcgtc agcggagcaa aaagggcacg cccactatgg
     6961 gtgggattct gattgttatc gtgctgaacc tgaccaccct gctctgggca caatggaata
     7021 cgcaggtgca attaaccctc ctttccgtcg tggtgctggc gggtttgggg ttctatgacg
     7081 attacgccaa gatcagcgag cagagcagcc gcggcacccg gtcgggcgtc aaactcgggg
     7141 tgcaaaccct gctggcattg tttatcgggc tgtatctttg gcgcctcccg tccacgcaac
     7201 gactgattac tgagatcatg gtgcccttct tcaagtatcc ggtcctgacg ggggccggtt
     7261 tggtggggct ggcgttgaca gttctgacca tagtgggcag ctccaacgcg gtgaatttga
     7321 ccgacgggct cgatggattg gcgattggct gcaccttgat cgtctccttt accttcctcg
     7381 tcctgaccta ccttgcgggg catcgaacgg cttccgccta tttgcagatt ccgcatgtga
     7441 tcggggcggg tgagttaacg gtattttgcg ctgctctgat tggggccggt ctgggctttc
     7501 tgtggttcaa ttgtcatccg gcccaagtat ttatgggcga taccggctcc ctggcgctgg
     7561 gcggtgcgct tggcatcgtc gcagttttga ttcatcaacc tctggtgtta atcatagccg
     7621 gaggggtatt tgtgctggaa gccatctcgg tgattctaca aatcggttgg tatcggtaca
     7681 cgaaacgacg ttctggaaca ggagctcgcg tgtttctgat ggcaccgctt catcaccact
     7741 ttgagaagaa agggtggtat gaatcgcagg tcgtcatgcg attttatatc cttggtgtgc
     7801 tttgcgcgtt gttggctttg agcaccttga agattcgcta acatgcgtgg accaaagaat
     7861 agaaaaagca gggttaatct tggacgcgcc gggcaggcga ttcgtgcgcc ttcttccggc
     7921 atgggcaggg catggccgct gggcgcatcc tggtgtttga gaaggaagct ttccggttcc
     7981 tccgtaacgg agaatgaggc cgcaaagtat tcttattgtt tgcgcgcaat cccgcgggcg
     8041 cctcaagcga cagggctcag cccaagctgc ctgcctggtt gccgcctcat tccctcttcg
     8101 cttctatacc gctgcgcctc tgatgctgga tcaaatatga tacggcatga ggacggtaag
     8161 aggaggaagg gcagagggtg gaaattggag gaaagaagcg gttggaaatg ccatgcgtac
     8221 aggcagttac ctctgagcaa agcaaaaaag cttaggcatt ttccgattaa gaactcgcca
     8281 gcctgtccgg agagagaagc ccgggaggca gaagttaatt ccgcggcccg ctttcgtgac
     8341 ggcggaaccc gttgccgcag gccttccatc tttggaacgt tatcgaaaac tgaagcctgt
     8401 tcgcaaataa cgcggaatgt ttttacccct tgcacgacga gtgattcgct gctagaagca
     8461 ttaatggaag tggcaaagag tgcggcttcc gacgacgtta ttttgttttt gccgactcat
     8521 ccgagcttgg ctgagtatag aaaccatcaa cgccacggga cagccctttg ccgggcagtt
     8581 aaatcaatat gtagcggtgt cccgtgtggc acccacaaga tagacggtag aaaagtgata
     8641 acagtagaaa aacaaagggt aatcaaggcg gctttctctt cgatttttct tcggggaagg
     8701 ctccggagca aaacgaataa acaatcaaca cctcaataaa ccagcgcaaa agcgctatct
     8761 gaaaatgaac aaccaaaatc cactcattct acaaggctcc gctatggagc agaaaaacag
     8821 aggacggaac cgtgtaaaga tcgcggtttt cttcgtcttg gccattcatg ggatcggcct
     8881 tatggctttg ctcatgcaag gttgcggaca atcgaaggag ccggtgcctc caccggaggc
     8941 aacagagacc aacgagctgc cgcctttcgt tgaacccact ccaccgccgc cgccaaccaa
     9001 tgaaccgcca gttgtggaag ccgctcccgt ggtgccggaa acgccgcccc ccccgccagt
     9061 ggttccagtg gaagtcaccg aatacactat cgtaaaaggg gattacctcg agagagttgc
     9121 ccgaaatcac catatctccc taaaggcgct cctggaagct aatcccggca tcgaacccac
     9181 acggctaaaa atcgggcaga agatccgcct gccagcagcg accgccccag cggcaactgt
     9241 agcgccgcaa acggcatcaa ccgaggctgc tgttggttcg ggcgagaaaa tctacacggt
     9301 gaaatctgga gataccctca cccacattgc ccggcacttt ggcacgactc cgaaggcgat
     9361 cagtgccgcc aataatctta agactaccag catcaaagtc ggacaaaagc taaagattcc
     9421 gaccaaggcc gccgcgttgc ctgccacagc cccgagtgca cccataacac cagttgaaac
     9481 ggtgccaatg gctccggctg caccgccgcc gccgactccg cccgaaacat aaaaattgca
     9541 acgagcgttc tggcactgcc cgcgggtgcc gaacgctact ttcgataaat gaaaatggcc
     9601 gtcaccacac tggtttcaag cgtcgccgcc ctgctggcgc tgggaatggt gatgctttac
     9661 agctcgagca tggcccaagt gggcgcgcgt tatttgacca tgcaattggc atggtgcgct
     9721 ctcgggttgg gagcctgcct ggtgacggca ttgttgaatt atcgccagct taaaaaaatc
     9781 tggtggctgc cgctgctcat tgcattggtg ctgctggcgc tagtgctgga tacgcacttc
     9841 gggaccagga taaacggcgc ccgacgctgg ttccgactct ggggcctgag cttccaacct
     9901 tcggagtttg ccaaaatagc tctccttatt gccttagcct ggcacgggga cttctaccgg
     9961 cgccaggtgt cgcgctggaa acgaggaatc cttgtgccgg gggcgttgat tgggttaacc
    10021 gtagggttga tcttcctgga accggatgtt ggcaatgcaa tggtcttggc agcagtcagc
    10081 ggtatggtgc tgttgatttc ggggataccc cttcgctatt ttcttctacc ggcactcata
    10141 gcggctctgg gagttggttt gtttatctac tataatccga tgcgttccgg gcgcatttat
    10201 tcctggctgc accttgagga aacccggcgg gacacaggct tgcaagctta ccagtctatg
    10261 gtggcgctcg gcagcggggg actcacggga aaaggtctgg gcgatggccg gcagaagctg
    10321 ggtttcgttc cggaacacca tacggacttt attttctcta ttattggcga agaacttggg
    10381 ctgatggtca ctttggcagt agtggcggcg tttgtcgcga ttatctttag tggggctcac
    10441 attgctcgaa atgcggggga catattcgga atgttgctgg cttcgggaat tacttttctg
    10501 attggcctgc aggcttttat caatatcggg gtagtgacta gcgcgttgcc caacaaaggg
    10561 ctgccgctgc cgttcattag ctacggcgga tccagccttg tcataatgtt agggtgcgtg
    10621 gggttgctgc ttagcatcgc ccggcaggca ggcgaaccgg attctgccat gcggggagga
    10681 ttgataacga acgattcggc aaaccccttt gcccgaggca atcagcctga accatgacgc
    10741 ccgccgcctc cctttcagtt caggtcgcca tcgcctgtgg cggaacggga ggacatcttt
    10801 tcccaggtct ggccgtggcc gacgaactgg tgcggcacgg ctgtgcagca acactgttta
    10861 tttcccccaa ggaagtggat cagcaggcag ttcgagatgt ctccggcatg gagattatta
    10921 ccttgccggc ggtgggttgg caacgcggca gaagcggagc gttcctgcga ggatttatcc
    10981 ggtcctatcg agcgaccgca aagttgttta aaacacgccg gccgcaagca gttttggcga
    11041 tgggcggatt caccagcatg ccgccaattc tggccgcaag aagaattggt gcgcgcacgt
    11101 ttattcacga atccaatgcc atccccgggc gcgccaatcg ttggctatcg cgggttgtgc
    11161 atggcgcgtt tatcggattt ccggaaacgg ctggccgttt gcgcgcgcgc aatctgagcg
    11221 tgaccggcac tccggtacgt cccgggtttg aggtccgcga ctcatccgcc tgccgggttc
    11281 aattggggct ggatccagaa cgtcccgtcc tcctggtgat gggcggcagc cagggcgccc
    11341 ggggggttaa tgaattactg attcaaactt tgccgctttt gaaggcagac ggggctgact
    11401 ggcaatggct ccatctggcg ggcgctggcg acgcgcaaaa agtggtccaa gcttatgcgg
    11461 gttatcggtt aaaggcgatt gttcgtccct ttttaacgga gatggaggtt gctctggggg
    11521 ccgcaacggc agcggtctgc cgggctgggg catcctcgtt ggccgaactg gcggcgttgc
    11581 gcgtaccggc ggtgctcatc ccttatccgg cagctacgga caatcaccag tttttcaatg
    11641 ctcgcgcgtt tgagtcgtct ggcgctgcat atttactcga agagaaaacg gcctccccgg
    11701 aagcactccg gcaactgctg ttggatctgg ttcataaacc tgcggtgcgg gaaaagatgc
    11761 gggacgcttt ggcgcgctgg cataccccct gcgcggcgga acagattgcc agcaccatac
    11821 tcgctgccgt agaagttgaa gccggtaaac gcaactatca cgctgggcag cttcccgcag
    11881 cccgacccgg caccgaccgc ggagatgctg cgactgcgcg aaatccatcg gcctcggcgg
    11941 aaacccagcc aatgcggata aaccatgtgg ctgattacga taaaacgctt ttgccagctg
    12001 ggagggtcgc ttgacaccac aaggaacgat tttggcaaag aagccgcaca gcggaaaaga
    12061 agcatcggct gaactgaccc ggcgcgtctc ggcagcgacc gcgattcgtt gtgacgaact
    12121 gatggccagg catacgacct tgcgcgtggg cggcccggca gatctttacg tcgaacccgc
    12181 ctcggaagca gacctggtgg aagtggtggc cttctgtcgg gagcaggctc ttccgttttt
    12241 tattctcggg cggggctcga atcttctggt aaaagacggc ggctttcgcg gcgtggtgat
    12301 tagtctggcg cacgcacatt tcagctggat aacggtggag ggcgagcgtt taaactgcgg
    12361 tgcgggcgcc agggtgaaaa tggttgtggc cgccgcccgg cagcacagcc tggccggatt
    12421 agagtttctg gaaggcatcc ctggcagcat cggcggtgcc ttgcgcatga acgccggcgc
    12481 aatgggcggc gcaatctttg accgggtcga aaccgtgcgc ttcatgagcc cggacggaca
    12541 ggtgcatgaa cgggtggctc aggagttgga accccggtat cgccgttgcc cgtttctcca
    12601 aacgcagatt gtcctttcgg cggtgttgcg cggtcatgcg ggatcgagtg aagcaatccg
    12661 gcagcgctta aatgagtata gcgaaaaacg aaggcaatcg cagccggcag ccccgagcgc
    12721 tggctgcatc ttcaagaacc cgggcactca ttccgccggc caactgattg acgaattggg
    12781 cctgaaggga acgcgtgtgg gcggggccgt ggtcgccgcc aagcatggca atttcatagt
    12841 gaatagcggt ggcgccacgg cacacgacat cctggcgttg atcgaaatta tcaggcggcg
    12901 ggtccaggcg gaacgcgcga ttgaattgga aatggaagtg gaagtgatcg gcgaatgaat
    12961 tcctcgctaa ccaagcttaa catcacggtc atgcttggcg gccccagcgc tgaacgtgaa
    13021 gtttccctac ggtccggggc tggggtggtc aaagcactcc gttctctggg ccaccaggtc
    13081 aacgagttgg atcctcagaa tccggattgg aaactgccgg aggggacgga ggtggtcttt
    13141 ctggcgctgc atggcaccta tggcgaggat ggtacagtgc agcaacggct ggaagagttg
    13201 gggatggttt ataccgggag cgatccggag gcgagccgca tcggcttcga caaatatttg
    13261 accaaacagc ggtgtgtggc ggcggggatt ccgaccgcgc ggtatttgtt gatcgaatcg
    13321 cttaccgcaa gctggccgat gggctgggac ccgccggtgg tgctcaagcc ggcccggcag
    13381 ggttccagcg ttggattgca gttcgtcgag cgcgtgtccg attggagcac ggccctggcg
    13441 gaggcaatgc ggcatgattc ccgggtgttg ttggaggaaa agattgccgg ccgtgaatgc
    13501 acggttggta ttctcgcgga cgaaccgctg ccggttgttg aagttcggcc caagtccccc
    13561 atctacgact atcaaaccaa gtatagcgtc ggatccacag attacttttg cccggctccc
    13621 tttgatgcgg cgatgactgc ccggattcag gcggcggggc tgggcgcctt caaagcgatt
    13681 ggcggccggg attattcgcg cgtggatatc attgtgcagc caaacggcga attggtggtt
    13741 ttggaagtga atacgctgcc gggcatgact gagatcagtg tgttgcccaa agccgccgct
    13801 gtcgccgggc tctcctaccc gcaactctgc cagcgcatgg tggaattggc acttcagcga
    13861 aagcatgtgg ttcaaacgca aacctagaaa ccggcggctg gggcgcgatc aggtgctgga
    13921 tgtccgatta cgctccagcc aggttcgcgc tgcccggacg cgtttggcag ccctcaccct
    13981 gggagccgta ttcggcaccg tctttggtct gtacctgctt tatcgcggtt gggactggac
    14041 gctcgaccgg ctcgtctata aaaacccggc ttttgccatc caacgaaccg acatcgagac
    14101 cgacggcaat atcgcgctgg atcaactgcg ccattgggcg ggaataagaa cgggagaaaa
    14161 cttgctggcg ctcgatctgg cccgggtcaa acgagacctg gaaatggtgc cgctgataca
    14221 attggtttcc gtcgaacgca ttctgcctca tacggtatgc atccgcatta ccgagcgtaa
    14281 acctgtcgcg caagtcaacc tcccgggatc ccgtccggga gaggccaccc cgagggctgt
    14341 gtatcatctg gacgcatcag gacatgtcat gtatccgctg gagccgcgtc aacgggctac
    14401 cccggccaac ccggccgatg aggcactgcc ggtgattggg ggcattgccc cgggcgaact
    14461 ccaacctggc cgctgtctta acgtgcccca ggcccaggcc gctttgcggc tgatcgaggc
    14521 attcaaagaa tcgccgctgg ccggcctggt gaccttaaag catgtggagg tagcggctcc
    14581 cgaagttctg gtcgtgacaa cgggacaggg gagcgaggtg accttcgcac taaccgacct
    14641 gaagcagcaa ctgcggcgct ggcatgccat ttttgagttg gggcggcaaa cgggcaacac
    14701 catcgcaacg ctcgatttgg ccattagcaa taatattccc ctgcgctggt ttgaggcggc
    14761 agcggaccct tccgcggagc ccaagccagt cagagtcact cactcccgga agaaaaatgt
    14821 ttaaccgttc ctccatcatc gttggcctgg aaatcggcac ttccaaagtt tgcgccgtag
    14881 ttggcgatct tgccgccgat gacgccctca acatcgtcgg cctcggccag gcacgctcgc
    14941 gtggtgtccg caagggcgag atcgttgacg tggcggtggc cgaagaagat gtgcgctgcg
    15001 ccattgtgga ggcggagaaa atggcggatg tcgaaattcg cagcgtttac ctgggagtga
    15061 cgggcagcca tttgcagggc ttcaataatc gcggtgttta tccggtggct tccgccgatc
    15121 gcgagattac tgcagatgat gtgcaagacg tgattcggag tgccaaaaca atcaacctcc
    15181 ccgccgagaa ccacactctg cacgccatcc gccagcattt cttcgtggat ggccaggatg
    15241 gcatccccga cccggttggc atgctgggtt cgcgcctcga agtggacgtc cacgtcgtgc
    15301 acggaaaccg caaccggttg cagaacgcca ttcgcacggt caagggactg caactggagg
    15361 tggacgacat cgtcttcaat ggtctggccg cctcgctggc attattgacc tccgaacaaa
    15421 aagaactcgg ttccctggtc attgacctcg gcggcgggac ggctgactat gccgtctgcg
    15481 ccaatggcat catcaagcat acgggcgtgc tggcggttgg cggcgatcat gtctcgaacg
    15541 acctggcgta tggtttgaag gtatcgctca gccgcgccga gcagctcaag atcgagcacg
    15601 gctccagttt cgtgactccg gatgacaagg ggcagtcgct cactcttccc aacgagcacg
    15661 gtctgccgca taaaaccgtt aatctcgagc atctgcgccg catcatgtcg ttgcgcctgg
    15721 aggaaatctt ccgccttatc ggccaggatc tcgagcaagc ggacgtcctg gattacctgc
    15781 gcgcgggggt gtttctttgc ggcggcgggg cgcacatacc ccggattact cagttggccg
    15841 aacaagtctt tcaagtgccc gccttcctcg gccagactaa ttcgatcagc ggcctgaaat
    15901 cggctctcaa ccagccggaa tttgccacgg caatcgggtt ggtcaaattc ggatcctttc
    15961 aacagcgcaa gagcggtgat aaatcatcct gggcgaacag tttcaaaaat accctgagcc
    16021 agcttttccg gtgcctataa cgctcccgac ctctcctatg atcacaagcg cacacaacga
    16081 agcggtggtg acaacacccc accggaccct ggccgtccag attattggcg ttggcggcgc
    16141 ggggatcact ctggtggaat tgatgcgcca aggcgaaccg tccggggcgg attttgcggt
    16201 agtggacacg gacgcccgtt cgctggcggt cagcggagcc aggaaaaagg tgcatctcga
    16261 aatcccgcac ccacgcgggc tcggggccgg caacgacccg gatcgcggcc gcgcgctggc
    16321 cgaggagcag ttttccaagc tcaaagcgct tttcacgggg gcagatatgg ttttcgtatt
    16381 ggccggcctc ggcggcgggg cgggcaccgg gatcggaccg gtggtcgccc gcgcggccaa
    16441 ggaagcgggc gcgctgacgt tgggctttgt cgcgatgccc tttgattgtg aaggccgcca
    16501 tcggcagcgc ctggcattgc aggggttgac gcagttcaag gccgccgctg acggcgccat
    16561 ctgcctgccc aaccagaagc tttgcaagct gatcgatgaa aacaccagcg tgctggagac
    16621 cttcaagatc atcaatgagt tcctggttac cggcgtgttt ggcgtctggc ggttgctccg
    16681 gcatcggggg gtcattgaaa tcccctttgc cgaactgacg gcactgctcc aggatcgcca
    16741 cactgaatgc gcgctcgcca ttgccgaagc gatcggaccc acgcggtccc gcgaagtaat
    16801 ggacaaactg ctggcgcatc ccctcctcga tggcggccag gtgctggtcg aagccgaaac
    16861 ggtgtgggtg aacctcacag gcggcccgga tctatcgatg gccgacatca accgcgtgat
    16921 ggggcagatc aatgaacaat gcgagcgcgc ccaggtaatc atgggcgcta cggtagacga
    16981 aaccttcaag gatcgcctgg ccatcactct ggtcgccgcg cgaaagcatc cggaatgcga
    17041 cgcctggacc aaaacggctc ccaactttgg agaaccggat caaccgcggc tctctcctct
    17101 gacgggggaa cgctccggat cgcgttttgc gtcggtggcg cccgtgctga cacccgagca
    17161 agttgctcag ctcccggcgc gacagggcag cggcgccttg cgtcagcgca agagcgcttc
    17221 ccgaatgcgg caggcgcagt tgccgctgga gatcgtctcc aaaggacgat ttgataagag
    17281 cgaacccact gtccacaagg gcgaagacct cgatgtgccc acctacatcc gccagggcgt
    17341 tccgcttaat taagaacggc cgcggaacaa cgctcaaacg ggttgggagc agccgaggca
    17401 taccctctgc gcgcgcctga ccgcgacgga ggcttacggt agtcggcgga ggcgataata
    17461 gcgcggagag gacaggccgc cggtttgcga gccatcgtcc cgaaattcaa aggtgccgtt
    17521 gacggaggtt agaacctcgg caaaaggagt ccagtccgga ggagagagcg aaggcgtcca
    17581 ctgcacctga aactggtccc tgaccgacgc cgtccactcc aagtacacgc cattggcctc
    17641 gaccgctatg cgggaaatgg caaccggcgc gccgtagtct tcgaggacaa gcccttcgcc
    17701 aatgcggaag aagtggtagg gcgagggcat ctccacgcaa taccaagcgg cgttcccgct
    17761 ggcggtcaac gtcggagata tcaccaccca attagtgctg tccaggtcag ccttgccctc
    17821 gacgtaatag tggaggccgc gcatggacgt ccagccgaga cagaaattat agctatcaac
    17881 caggcattcc gtaatgagca aatcaaggcc ataagccggg aattccgtcg cggtgatggc
    17941 ataatccgcc ggctcgccgg aagcgttgac ggcggcaata aaccaatccc ccggcgtcaa
    18001 cgagaccggg cttgagaagt caaagagcgt aatcagttcg tcgtttaacc cggggttggc
    18061 gctgcggcaa tcatagctgc ccgcattggg cagcggcagc cctttgcgcg cgagcagggt
    18121 catatcggcg accgggccga taatctcaaa ttgggcgcgc accgcattgg tcgaaaccac
    18181 gaagtgataa taattggctg ttccttcccc cccggagtta tagccgtaat agggttctcc
    18241 attgtttaac ctgactacgt tcgggaaggc attggtgtat tcggtggccc ggatggtata
    18301 ggcgacattc ttcaggtcgg cattgaacac cccgagatgc caacgcccgg gagcgagcgc
    18361 aacgggtgcg gtgttgggga acacgaaaat gccctcactg ttagtgccgg gacagaaact
    18421 tccgtaatcg aaaccggcca gcgtcgggag cgcgtcgccc cggcggacga ccaggttcag
    18481 gttgccctcc ggattcaaga gctggaacga aaccgcagtc gcattggacg aaacatcgta
    18541 agcgaagtat tgaggcagca ggttgccggc atgaatggcg ttgaacggca cgccattggc
    18601 cagggggatg aggtcaaaat ccacccgcag ggcggcggaa acgtcattcg accccgggtt
    18661 ctgcagcccg aggtaatagc gcatgccggg ggccagcgga ggcaacccgt ccatatttag
    18721 cgtgtgaaat ccctccgttg cctccgcgag caaagtaata tcgccggcgt tggtgccggt
    18781 aggcggccgg ttctggttga acagcacgtt cacgggcccg gacgcctgaa caagcagatt
    18841 cgtcgcccgc gtagcccagt cgggcgcctg gacggcaaaa ggagccactt gcccgggagg
    18901 cacggtgttg gcgcgcgcga tcccgggagg caactcaacc ggcacgggct cggtgttctg
    18961 aaaaacaatg cgcaactgcc aactggccag ttccggcgcg ggcaaaccag ctccggcgcg
    19021 ggaatcccac atttccagtt gccaggtgcc gccggcgcgt tcgccgatca gggcgctcag
    19081 cgattgctcg ggccggcagt aaaaatccca actggactct tcgggtagat acggaggccg
    19141 cgcaaacttg attggcacct gcgtccggtt cgtatcttcg gtgaacacaa cgtagccggg
    19201 cccccaactg gccacggtcg ccgtgtattg ccagagcgta ttggtgtcct cgttgttccc
    19261 ttcattcatg acaatgacaa gctcggtagc tagattcgtt tgaacgcccg ggccgaaggg
    19321 aatcacgacg aggttggtgt cgctgaccaa accgctttca tagatcagct cctgcccata
    19381 atagacgtgc agcgtgtcgg gcgcggcgta gaaatcatag tctatcgtca gaatccccgg
    19441 gttatacccc acgaacaggt tcgtccggtt ggcattgtag tcgccggaat aaaaggtggg
    19501 ataaatatta gtccggagca caatcccgcc cagttcgtcc gccgtgtccc cgccgcgccc
    19561 atcgaacagc cgcacgcgcg taccggcagg gctgatcaag gtgaacacca tatcggaaat
    19621 ccgcgaatga ttcgtcgcca gcagcgccac ttcgattgcg gcgatgggcc gggtgtcgga
    19681 aaggaacagg caggcattcg tcagggcatc atccggaaga gaaacagcgc cggggccggt
    19741 gctgatgaca ggttcgccgc cgcccggatc cacgccgacg ctgctctcca ggcggagggt
    19801 ttgcgggaga gcgttggtat tgaaaacgcc gatataatag cggcccggaa ccagcggcgg
    19861 cagggtgtaa ggggtgaaag accagcaggt ggtggcggca tccgccagca accggcgatc
    19921 aaaaaccgtt tgcgacggga cgcccccggc gcgcaggtaa acctccagcg gcaaaggttc
    19981 cggggtatcg ttatagaggc acagggtaag attcgtcgcc cccaggggaa tctccaggaa
    20041 gtcgccggcg aaggcatgag cgggcacggt gaatggcgtg ccggcctccg cgttttgcgg
    20101 gtccagccgg atagccaggt tctggaccag acctgtctgg ccgggctcat aattggcgag
    20161 gttcagcagc caaatccccc ggcccggctc gccgatgaaa tcacgcaaac tgccgggccc
    20221 atcggtggga accgcgccaa ggatatcgct ttctccgttg tcctcataga cccaggtgta
    20281 ttcatgggga ggcggttcgc cgggaggctg ggaacgattg tggagcaccg ccgttttgct
    20341 gccatgacgg agcgcgcaga tgaggttggt aaagccggcc tgccaaagct cgttggtaac
    20401 cacaacgcgc cggaccgcca ccggcgccgg agcaaccgca atcacgttgg ctacgcccgg
    20461 ccgggccgcc gccccagccg gaatcgtcgc tggcaggtta atgcctcgca acagccaact
    20521 gccgttcgcg tccggctcgc caaagggctg ttgactgaat acccccataa acatgtattc
    20581 ggcggctagc tggctttccg ctttgacgcc gacataataa agcgcgcccg gcgcggcatt
    20641 gcccaaaaca atcgcgtgcg tgccgccgcg tccgagggcc ttatgagcgg ccgccagggc
    20701 tgcggagccc aggttggtca gcgcggaatc cgtggaaacg tatagatcaa tgtccgcctc
    20761 cgccggggcg gcaggattca gattggtctg gaaaaggccg ggcagggatg gcgagagatc
    20821 ggccgccatg aacgtcagga aggcggcatg gggatagttc tgctcattgg tcaggacgta
    20881 aaagcgccat tggttggtta ggccgacgcc aatctcgccg ctggcaccgc ccgcccaggg
    20941 aatgctgcgg tcgtccgcct gcggccccat agcccccgcc cgttgattca gcaaaagccc
    21001 gccggagatc ccctgcccag gctcgaagct atttgtgacg accatcaccc ggggggaatc
    21061 ctcggagacc aggggcgtgt cggtgaccgt cagggcgtcc gcttccgctc cgtccccgct
    21121 ggcgatcacc agcgcataat cctgaaccac gccattcgtc cgggcggaga cggcattgac
    21181 gttcaccgcc cgggccgaaa ccgtgacgga atagctggcg cccacctggg gcgggagata
    21241 gacattctcg acgttgttca cgacatcggc gttgggcggc tgagccgggt tccatgcctg
    21301 gttgacgctg ccgccggggg gaatgtcgtt gcccagatag acttcgcccg tatccaggtt
    21361 ggtgacgatt aaatccaggt cattcacgag cttgaggctc gcggcggggt tgcccggggg
    21421 atcggtccaa acaagtgtga tgcgcagcgg ggcattggtc gccggggatg aaagcgaaag
    21481 gaaccgggtg tggcgctggc cggtagccag ggcattggcc ggatcctggt cgaacacatg
    21541 catgggggcc gcgaccccgg caaaggcgtt ggaaagcgcg gggggcaagc tattggtgag
    21601 gttgatcaag ccccagccgg acgcattggt cggcatgtgc ggcgaaaaag cgcccggcgt
    21661 ggcgagcggc cgcgcgccgt tgatcaacag tgccttcatc aacgcggggc tgttggtgcg
    21721 gcccaggcgc aggaagaact cctgcatgag ggccaggctc ccggaaaccc gggcggcggc
    21781 gaggctggtg cctgattcgt agcggtagaa aggcccgagg gtctcgttca gattgctgag
    21841 caccgcggaa tagttgttgg acccggcggg cgggccatag taagcacccg aatcccattg
    21901 cgtcgagcga gcggaaacga caaacacgcc gggagccaca atatcgggtt tgacgcggcc
    21961 aaacgcgcct tccagcccca cgcccacatt gccgcggctg gagaataccg ccatgacgtt
    22021 gttggtgagg gtctgtccca gccagggtga gttggtctgg caatgattcg tctcattgac
    22081 gacgccgcac tcccaggctt gcgccgcaat ctgccgcgcc tgcgcaaccg cgcccacggt
    22141 gatgacgttc ttggccgtcg ccggcgattg aatcgcatcc gcgtcgccgc ccgtgccgtc
    22201 ctcgctgcca ttgccggcgt tgcccgcccc aaaaaccagc aataacggct gggcgcccgg
    22261 ctcccccggc agcgcgtcgc ggacggcggc gtcatagcgc gccgcggcca gatcgtagct
    22321 gggatcgccg gcatagtgcc agctatggtt cgagatgaaa gcgccgtgcc gcgcggcggt
    22381 ctgttggaga taggcatccg aagccggccc ggcttgagcc gtcagggcca ggacttgagc
    22441 cgcgggagcc tggccgcgaa attggagatt cgtgccgggc atgaccgagc cgctggcctg
    22501 ggtgaccgtg ggagactgca gcccactgcc ggcaataatc ccggcgacat gcgtgccgtg
    22561 gccattcgta tcaatcccgc tcgccggcgc gtcgcacaga acccgccccg ccaggtcggg
    22621 atggtttgca tccacaccaa aatcagcgac gccaaccagg acattgctgc cggtcagctc
    22681 cagataattg accggggtga tcgcgtcggc ggccgcggcc acctcgcacc ggcccaggtc
    22741 attggccgca acgcgggcgc gcgcgagctc cacctgctgc acgcccggca ggcgcgccag
    22801 cgcgggcaag aggtcggatg ccccggtcga agaatcagcg cgccggcccc gcacccaaag
    22861 caccggccca aacggcgaag gtccatcgcc aacggcctcc actccgagat cccgcaattg
    22921 gtcgagcacc tccgccgcgc tatcgggaaa gagcgtcagg tgaagcggct gcgcctccgg
    22981 caaagcctgc cgctcaagcg ccagggccag cagcggcggc ttgagtttga agtacggctc
    23041 gtaaggcagg acggcctgga cctggggatc ggcttgcaac cgctgcgcaa cggctgccgg
    23101 cgcgccaacc aggtacgcct ggttgggaat gtaggcaatc atggcggccc cggcggcccg
    23161 caggcgtatg cgaaaagcat cgtccagcgg cgcgcgcgac tggacaatat aggcgccggg
    23221 atcaccggac gcgcgcaaat gatcgggaat ggacaacgcg agcggctggg cagtatcgag
    23281 cagggcattt tccagcagca aagccgtagg ccggcgaaca agctggcaga gtggcgtggc
    23341 cgtattttcc aggcgcaaag atgaactgcc cgcgcggctg ggcgtccgga gcacgggcgc
    23401 cggtgcggcg gccggcaacg cggaaagcag cgcaggggca accgcgtctg ctgccggcga
    23461 taggtggact tccgttgccg ccccccgctc cccggccggc tccgacgccc gctgcttgtc
    23521 ccgggggcca atgcaggcgg ccaaggcaag cagcatcgag ctcgacagga gccagatcag
    23581 gaaccgagag cgcataggac ggcccaatct accccacgcc cccacccgct gaccagccaa
    23641 aagctttcag ggatggagac gccttccggc ccatccgcct tgggtataga ggcggttgcg
    23701 gtcgggggca aaccatcctt acttacgctg ggccccctct ctacccggat ggaccggaat
    23761 aaaacgagtt gtgtccgtca agttcagcaa attggtcgtc atggtttcca ctcaaggtg
//
LOCUS       DAOPQG010000056        23690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2690_length_23690_cov_16.730611, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000056 DAOPQG010000000
VERSION     DAOPQG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 23690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23690
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_23690_cov_16.730611"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PKI07_07985"
     CDS             <1..1001
                     /locus_tag="PKI07_07985"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC51032.1"
                     /translation="FGKGAKWRRAADFASFLALASLRLARLPRPEVVVTLTSPPLISF
                     LGAGLARLRRSRFFYWVMDFNPDEAIAAGWLRPHSPVARVLDWISRFSLRQARKILAL
                     DRFMRERIVAKGIAPAKVLVIPPWSHDTEVTFDAEGRERFRKAHGLDGKFVVMYSGNH
                     SPVHPLDTLLAAARQLASNPDVTFCFIGGGSEWRKIQEKQKTDRTLSNIICLPYQPLD
                     QLAGSLSAADLHVVVMGNDFVGLVHPCKIYNALSVGAPVLYIGPSPSHISEILDTLDG
                     HYTCAAAHHGDVGRVVEQILRVRRESAALVRQPPKISSMFSRETLLPRLVAELEGQ"
     gene            complement(1821..3656)
                     /locus_tag="PKI07_07990"
     CDS             complement(1821..3656)
                     /locus_tag="PKI07_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOC51033.1"
                     /translation="MREEFEKMAAAGKLERHLIPALVQLTDTGFCLHRSWGFGRIKTL
                     DPVFARFTIDFDNKPDHSMDLAFAAESLKPIPKDHILARKASELENLRKMAATQHLEL
                     VKLVLTSLDGKATVEQIQKALVPEIVTSDWPKWWETARRELKKDGHFHVPLKKSEPII
                     YEDEEISPQQRLMTAFRTAKGLKAHSSTALEVIKNVDDLTDPAAAGAEIVALLNTEIA
                     SHLRTQPAVALEAIFVRDDVRAAIGLQPAAEESTPQTVWTQASEPGLLLEQLPNARRR
                     RALESFKTNFPDRWLDVLQLAFNTVSARTCGELAGFLIQNNHLEELKETLARLISQHS
                     ASSELLLWLAKERNDSFADILGPEVFRAMLTALERDQFNERRSNRLRDFIVDDPSLLL
                     ELISSADFEVIKDLTRALQLSPSFDDMDKRSLLARIVKSYPTMQSLISGEPTRQESTL
                     VVSWESLERRKHEYHELVQKKIPANSREIAIARSYGDLSENHEYKAAKEMQKLLLRRK
                     ADLEAELVRARGTDFAGVRTDVAGAGTVVYATDLATNQSERFTILGAWDSDPERNIVS
                     YLSPVAQALIGHKVGDEVEFEVHQVAHRHRLDAIEPYKTEEPSPA"
     gene            3708..5006
                     /gene="rsmB"
                     /locus_tag="PKI07_07995"
     CDS             3708..5006
                     /gene="rsmB"
                     /locus_tag="PKI07_07995"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOC51034.1"
                     /translation="MRAEKPREIALRLLQREAGGDFIENRLAAALARGRLSAADRHLC
                     QELAYGVVRWQAALDWLIARKTDARQLKPALRALLRLGLYQIFWLERIPPHAAVHETV
                     ALARASRLHAQTGFINAVLRGYLREQEATRRLLAELKTRQPHLGYSHPEWLVTRWRER
                     WGPEPAAKLMDWNNTPPRVFARVNTLKTDADRLLAQWRAEEVEYGLVRRDWLAENLVF
                     ELRAHPPLSRLPSFQQGMFYVQDPSTLLAVSELGAQPGERVLDLCAAPGGKLTALAQA
                     MRNEGRLFAHDTSPERLDLIRQNCARLGVSCVQPQTPADLEASGAAAGFDRILLDAPC
                     SNTGVMRRRVELRWRVQPEEIDRLQAQQRELLRQAAAWLKPGGALVYSTCSLEPEENA
                     GVLQEFLRQHPGFKLERTRELLPFVDGVDGSYVARISRGS"
     gene            5402..7330
                     /locus_tag="PKI07_08000"
     CDS             5402..7330
                     /locus_tag="PKI07_08000"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOC51035.1"
                     /translation="MATKLVLSFLLIILLVSGVFGLVGARVIGNRVVAEAQAKVHTDL
                     NAAREIYLNKLAAVNDVVRFTADRVLLLNSLLSGDTRPAWEALVRTREREKLDVLGVT
                     DRNGRVVLRATSGSVVGDDQSQDELVRAVMERAEPVAAACLVSAENLRRESPALAEQA
                     HFQFIETPKARARAATEESSGMMLKAAAPVLDSSHRVLGIVYGGVLLNRNCEIVDRIK
                     QTVFQNLQYKGKDMGTATIFQDDLRISTNVKNEDGTRALGTRVAEDVYEQVVRHGRPW
                     IGRAFVVNHWYITAYEPIRDLTGRIIGMLYVGLLEQKYVDLRHRAVMVFLAVTVVGAL
                     IALGLASFISGKISVSIKKLAVAAEQVAHGNLDTRVEIRSHDELLELADAFNFMAAAL
                     KKRDEKVREFTTRRIMQSERLAHIGQLAAGVAHEINNPLTGIVTYSHLLLERASADDG
                     TRASLEKIVKQADRCRDIIRGLLDFSRQRKPEKRQSNVNRVLEECISLVDNQVLFHNI
                     RIVQHLSANLPPVFMDPSQIQQVFMNMILNAAEAMHGGGRLTLTTRHVPAEQAVEVEF
                     ADTGHGIKEADLDRIFSPFFSTKEVGHGTGLGLAISYGIVKEHKGTITVESEEGRGAT
                     FTICLPVSNEEENLPRAA"
     gene            7320..8444
                     /locus_tag="PKI07_08005"
     CDS             7320..8444
                     /locus_tag="PKI07_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOC51036.1"
                     /translation="MPLKHTILVIDDEEVVLDACTQILDSRDCRVALANNGAEGLKRL
                     EELQPDLVFLDLKMPGLSGLEVLERIRAKLPLMVVIVITGYATVSSAVEAMKKGAFDF
                     LPKPFTPDELRLIARRGLEHSRLVQQTTALRREKEMLRENFAAIVSHELKSPLGALQQ
                     NLYALAAELEDKLAEEQQQRFARLQARVDDLLKLIHSWLRVYSVDINKLKETFQPVAL
                     ADIVARAVEYTQPHATRKGLQIETTVAEGMPSVIGDAGSLTEVVTNLLGNAVKYSFPN
                     HKIRVRVETRNAEALIAVADTGVGIAKEDVPRLFQDFARGQAQPEGATGCGMGLAISR
                     RIAEVHGGTITVESEPGQGSTFTIYLPLGQGVVQSSPTPV"
     gene            8455..8877
                     /locus_tag="PKI07_08010"
     CDS             8455..8877
                     /locus_tag="PKI07_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC51037.1"
                     /translation="MSEAKQLLIIDDDPDFVAGIKSILVKAKYAVDVVHNPKDGLQAL
                     KTKNYDLLLLDVMMGRGAEGIMIARKLGKDPKLRKLPVLIITGIREQMAFLFPGQAVH
                     PSFVEVDELVEKPVDPKLLLDKVASLLKTAETRKAKSK"
     gene            8874..9494
                     /locus_tag="PKI07_08015"
     CDS             8874..9494
                     /locus_tag="PKI07_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC51038.1"
                     /translation="MSTATAVAEPAAPSAVPPENPMSFVEEINHLLYASKGNPINTCI
                     QCGTCSATCPVASGGFMDNSPRRIIALIRAGFKEQVLKANTFWYCASCYQCTVRCPRN
                     IDIAELMYGLKRYTVWKTEHQEGLIGPVFSETFVKMILRTGRSYEPVLAPTYMFTYGL
                     PEMIQEATMGTALMLKGRLPLLPARIKRLDNFKRMIKHIIPLGDKE"
     gene            9491..10390
                     /locus_tag="PKI07_08020"
     CDS             9491..10390
                     /locus_tag="PKI07_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOC51039.1"
                     /translation="MKSYAYFPGCSLEKMAHSYHISALETSRALGVELRELEDWNCCG
                     ATAYFHVDELLAYTLCARNLAMAEQQKLPMVAPCSGCYKNMYFTREHLRHDADLAEHI
                     NAALAEDNLSFSGESKVHHLIEVFVHDVGLAQVKASVKNPLKGLRVAPYYGCQILRPR
                     KQARENVEQPTFFEDLVAACGAEPVPYPLRLTCCGGALIITNREAALSMVRNLLESAI
                     QARAAVIVTACPLCQVNLECYQKQVNKEFGTDYKMPVMYFTQLMGLALGIPPKRLGIG
                     SELVAVMPAIREAQEAAPALAAK"
     gene            10405..11178
                     /locus_tag="PKI07_08025"
     CDS             10405..11178
                     /locus_tag="PKI07_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOC51040.1"
                     /translation="MNLYVLLKMVPDTVEELEVAADGKSLDSEFLRFKLSEPDDHALE
                     QALILKEKMGGKVTVVAFDAPEVDDVLFTALAKGADRAVKIPLNQASLGTVASAKVLT
                     EFFAAAGALTPDSLLLPGSQAIDDLEGELAPLLAAALKLPYIGVVCGVTGDAGKVTVL
                     KEFAGGLRGEFEVTLPAVLGIQSAEKPPRYVPVAKVRAVMKSAQIEEAALASPEQPIQ
                     LDIERMYKPEAAGRAQMIEGSTEEVADQLVEVLAKNSVI"
     gene            11191..12162
                     /locus_tag="PKI07_08030"
     CDS             11191..12162
                     /locus_tag="PKI07_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOC51041.1"
                     /translation="MANPILVLAEQLRGEISDITFEMLGVGRALASAVGAPLYAVLLG
                     SEAAPLAAKLGKADKVFVADNPQLNLAPAATVTAVLKAVIDQSQAGLVLVGCTNVSLG
                     IGSTLAAQTGLPLVNFCRAARADGSAIQLTSQLFGGKILSDVKLPDGRGIACLYPGAF
                     PADDGKSDQTPPVEKLDPPMETPKVTFKQYIEPETGDVDITKQNILVGVGRGIQSQDN
                     VQLAEDLAQVLGGAVCASRPVIDQGWQPLSRQVGKSGMTVKPRVYFALGISGAPEHWE
                     GMQNSQCIIAVNTDPKAPIFDGAHIGVVGDVLELLPVLTDKVKARKG"
     gene            12398..14383
                     /locus_tag="PKI07_08035"
     CDS             12398..14383
                     /locus_tag="PKI07_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOC51042.1"
                     /translation="MAFGVFGRRAWQLIGLLRQGRYENRFDHPVRRVLHAVRHVLWQP
                     RIFNERSIGLPHFLIFWGFVVYALCFNWSLVRGLFPFLPVPYPDEVNLTSFFLEVFSV
                     LVLLSLGVAIVRRLFFAPKYLHLSLDANLILGLIGLLMCSMVFFFAFRMVHEGGAASA
                     WLPFSSQLARAFAGLSPETAGTLTQAMWWLHMIVVLFFLVYLPYSKHLHLLASPFNVF
                     FSQAATRPVGDLGVLDAKDDLTSGASRWQELTWKQLLSGFSCAECGRCDRSCPATASG
                     YPLQPQQIIQKIKDHMVHTALNGGNGEGAPKLIGDVITEAEIWACTTCMACMNCCAVW
                     NEQVPLIVTLRRHLVSQGEVERTVQDMLANLQRYGNSFGKSDRMRAKWTQTSGLKIKD
                     ARKEAVEYLWFVGDYANFDPRCEDITRQTANVFARAGVDFGLLYEAERNAGNDVRRVG
                     EEGLFEMLRDKNLQALGKAQFRKIVTTDPHTYHTLKNEYPWNGHRPEVWHYTEVLAQL
                     LEAGRLPLQKKLSGRVTYHDPCYLGRYNGVYEAPRRVLRALGVELVEMPRCRDKSFCC
                     GAGGGRIWMEDTEKIAERPAENRIREAAALAGVTTFVVACPKDIAMFRDAVKTAGQEG
                     KIVVKDLAELVAEAMAEPRPEAPSPKPETNPKPESPK"
     gene            14409..15602
                     /locus_tag="PKI07_08040"
     CDS             14409..15602
                     /locus_tag="PKI07_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOC51043.1"
                     /translation="MLEPAVVDKTTTLETLLQTPAGRRMMSCIQCGTCGGTCPYGEHM
                     EFSPRRVIGMLREGFLDEVIKSDSLLCCVACYACMAKCPRGIRLTDILLPLVKEQVLI
                     TRSEMPAEFQKSLENIHRYGNPMGESPRKRAAWTATAGTKIPVLAEIKREVDVLWFVE
                     CYTSFYARGQDNSRATAKLFQALGVDFAILGNEEKCAGDCGQMTWESGLFESLAEYNV
                     KLFNKYKFRRLVTGDAHAYNAFRFRYPMYGMTQPAEHTTQFFARHLDQLKPRLKKPLN
                     LTVTYHDPCCLGRADNIYDEPRALLRAIPGIKLVEMVHHRVNAVCCGGGGGGMWLDTY
                     YKQQGMDRLSERRVKEAVATGADVLAVACPYEVSRFEDALKVVPHNKPMVVRDVVELL
                     AESLE"
     gene            15618..17660
                     /locus_tag="PKI07_08045"
     CDS             15618..17660
                     /locus_tag="PKI07_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOC51044.1"
                     /translation="MKPIVPKKNEPIRIGYYICHCGTNIAGMVDVEAVAKYVATLPNV
                     AISRNYKYMCSDPGQELIQQDIREHRLNRIVVEACSPLLHELTFRKATEAAGLNPFYV
                     QMVNTREHNSWVHTDRTEATRKAMALARAAIQRVPHHKPLEVKKVPVNPNVLIVGGGI
                     AGIHAALTLANAGKMVYLVDKEATIGGHMAMFDKTFPTLDCAMCVLSPKMAAVGSHPN
                     IHLWTYSEVAKVDGYVGNFNITVRRKPRYILEDLCTGCQECVDACVFKEPKFPDKFNL
                     GLGKRKPVFLPFAQAIPPVVMIDPEVCLNFKRGKNPDGTHTVSDKCKKTCVQACGDRK
                     AIDFKQTEEFKNITVGTIIMATGFQIFDAKRTPFYGYGVYPNVYNALEIERIINASGP
                     TEGELIMRNGQRPKKVAIVHCVGSRDENTNRWCSRVCCLYSLKLAHLIKEHTNAEVYN
                     FYIDMRTPGKSMEEFYNRIAEEGMHLIRGKVADVYPDPNNGATGRLVVQAEDTLTNKI
                     LKIPVDMVVLGVGLEPQPDSENLRRQFNLSCSSEGWFLERHPKLAPVNTFTDGIFLAG
                     CCQGPKDIPDSVAQAGAAASEAFALIDKGHVEQEPNTAYILADECSGCKSCIPLCPYT
                     AITFDEERKKAIINEALCKGCGTCVAACPSGSIVQNLFEDEEIFSEIDGVLAPVPV"
     gene            17686..18252
                     /locus_tag="PKI07_08050"
     CDS             17686..18252
                     /locus_tag="PKI07_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOC51045.1"
                     /translation="MTNQTKPSASGETWTPRIVAFFCNWCTYTAADLAGVSRMKHAAN
                     ARVIRLMCSGRVDPQFIVEALAKGADGVLIGGCHPNDCHYVEGNYKCLRRYQMLKRMI
                     ADMGIEPERLRLEWISAAEGEKVKRVINEMTETITRLGPLGIPQKFDEWDREMKHFAE
                     HVAAREAAAGSACAEHGGKPESLEPAHA"
     gene            18245..19219
                     /locus_tag="PKI07_08055"
     CDS             18245..19219
                     /locus_tag="PKI07_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOC51046.1"
                     /translation="MPKPKIAFYWCASCGGCEETVVDLAEDILGVVEKVDIVLWPVAM
                     DFKYKDIEAMPDKSIAATLLNGAIRSDEQEEMAHLLRRKSQYMIAYGACAQLGGIPGL
                     ANQFPREQLLKFNYEDAPTMVNDKKTRPQLTTSDNGREPTLPELHNVVRSLDQVVEVD
                     YYVPGCPPTPKVTRTALTALLENRLPPKGTVIAPDIALCDECPRKATKPANIAFTEFK
                     RPHQALMDPNLCFLAQGVVCLGPATRSGCNAACTVGGMPCTGCFGPTSRVRDQGLKIL
                     SSICSNIAPKEEGEIEKVIEGIPDPVGTFYRYGLAKSLLRRKATLPSK"
     gene            19294..20760
                     /locus_tag="PKI07_08060"
     CDS             19294..20760
                     /locus_tag="PKI07_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOC51047.1"
                     /translation="MGRRRFTIEPITRLEGHGKIDIFLNDKGDVERAYMQVPELRGFE
                     VFSLGRPAEDMPQITSRICGVCPTSHHMASTKCLDDLYQVEPTVPGRKIRELVYNAFI
                     LEDHALHVFVLGGPDFIVGPEAPPAERNIVGVIGKVGLEAGKKVIASRRRVRELIAYF
                     GGKVVHPVLGLPGGVSKALDPADLPRFKELARDAVEFAEWTLAVLKQIVLENPEYLKM
                     VTSDAYTHKTCYMGMVDEQNRVNFYDGKLRVVDCNGKEICKFPTRQYLDYVAEHVEPW
                     SYMKFTYLKQRGWKDFVEGPDTGIYAVAPLARLNASDGMATPKAQAAYEDFYKTLGGK
                     PVHFTLANHLARAIEMIYAAERMQELVNDPDIINPDVRRVPTSIPKEGFGVVEAPRGT
                     LFHHYRTDERGCITMANLIVATQNNAARIAMSVDRAAKGLIKNGKVTEGLLNKVEMAF
                     RAYDPCLGCATHSLPGHLPLRVNIYDRQRNLVEQLVQD"
     gene            20828..21346
                     /locus_tag="PKI07_08065"
     CDS             20828..21346
                     /locus_tag="PKI07_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOC51048.1"
                     /translation="MLPLAPEMTAAPEPSSAPARRTLLLGLGNDILTDDGVGLYVVRQ
                     LRATLAGCPAIDVRETMEMGLALLDFISGYETVVLVDSIQTGRVAPGVVHELDAAALR
                     QLTGRTPHFVGIGETLALGRHLGLPMPQQVRIFAIEVADPFTLGTALSPALQAALPAI
                     VERVGRALRSLA"
     gene            complement(21382..23490)
                     /locus_tag="PKI07_08070"
     CDS             complement(21382..23490)
                     /locus_tag="PKI07_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOC51049.1"
                     /translation="MSAVNRFLIRLPRLLSKRLWAVPLAGALILGIVGLWVVKRVEET
                     TRGELAARLQTLLQADVNALRLWFTERQYAAKSFASDARVEAAIMQLAALAQDPRATP
                     QSLADSAPAQTLRHYLQPLLEAQEYLGYVVVAPDKRILATTGPEEIGVRAPTAYNLFL
                     NKVLDGQLATSRPFVSQTNLSRRVEGPTMFVAAPIKTTNDVVAAVLGLRMRPEKEFTS
                     IFSVAQIGQTGEAYAFDRSGLMLTATRFDQSLKTLGLIPPGRTNTAILNLKLLDPEGE
                     LHPGVPHPKPRSQLHLTRMAASATMGNNDFDVRGYRNYRGLKVIGAWAWLRDCGMGVA
                     AEQSLDEAFQTLYVLRRVFLVLFGLLLASGGAIFGFTLLVEHLQAAGRKDALTARRLG
                     QYVLMQEIGRGANGMVYRARHSLLRRPVAIKLLNPDQTNETNSARFEHEAQMTSQLTH
                     PNTVAIYDYGRTPEGLFYFAMEYLGGIDLDQLVRRFGAQPEGRVIHILRQACGSLAEA
                     HRIGLIHRDVKPANIILTRRGGVCDLVKVLDFGLVAAAHPGAAFGPAPRDIVGTPHFM
                     SPEAVEKPESATSQSDLYSLGAVGYWLLTGKTLFDSDTVEGLFSRQVSSPPPPPAERL
                     GQPVSADLAELLLRCLAKQAAQRPASAEALDQALAACASAGTWTGRDAEQWWMQHLAD
                     IETLPATVMSEKTLVIAPRS"
BASE COUNT         4529 a         7105 c         7622 g         4434 t
ORIGIN      
        1 gatttggcaa aggcgccaaa tggcggcggg ccgcggattt tgccagtttc ctggcactcg
       61 cctctttgcg gctcgcgcgg ttgccccgcc cggaggtggt ggtgaccctg acctcgccgc
      121 cgttgatttc tttcctgggc gccgggttgg ccaggctccg ccgcagccgc ttcttttact
      181 gggtcatgga tttcaatccc gacgaggcca tcgctgccgg gtggcttcgc ccccactcac
      241 ccgtcgcgag ggtgctggac tggatttcgc gcttcagcct gcggcaggcc cgtaaaatcc
      301 tcgcgctcga ccggttcatg cgcgagcgca tcgttgccaa gggcatcgcg ccggcgaagg
      361 tgctcgtcat tccgccctgg tcccacgaca cggaggtgac attcgatgcc gagggccggg
      421 aacgattccg caaggcgcac ggcctggacg gcaaattcgt ggtcatgtat tcgggcaacc
      481 acagcccggt gcatccgctc gatacactac tcgcggcggc tcggcagctt gcgtccaacc
      541 ccgacgtgac tttctgcttc atcggcggcg ggagcgagtg gagaaagatc caggaaaagc
      601 agaaaacgga ccgcaccctt tccaacataa tttgcctgcc ctaccagccg ctagaccagc
      661 tagcggggtc cctgtccgcc gcagacctcc acgtggttgt aatgggcaac gacttcgtgg
      721 gcttggtgca tccttgcaag atctacaatg ctttaagcgt aggcgcgcca gtgctgtata
      781 ttggtccttc gcccagccac atttcggaaa tcctggacac gctggacggc cactatactt
      841 gcgccgcggc gcaccacggc gacgtgggaa gagtagtgga acagatcctg agggtccgcc
      901 gggaatccgc cgcgctagtc cgccagccgc ccaaaatcag ctccatgttt tccagggaga
      961 ctcttctgcc caggctggtt gccgaactgg agggacagtg atgcactgct agtcgttgag
     1021 ggcagcgagc cccgtttcca attcttggct aaggccggag tcttgttcgc aggattccgg
     1081 cctttttctt gccgcgaagg gttttgcctc gaaactacag tattatttaa catgctctgg
     1141 ccaattcaag ggcacggctg ctggatccca ggtctttcgc tggtaaaatc aatagatcag
     1201 gcataaaagg gggtaaaata gaggcaaaac ccgaatcatg ccggctttgg cgcaccttga
     1261 agatcccttt gatttaccct gcctttaagc cgtgtacaag gggtgtacaa gggatgctca
     1321 ccggaagttc ataggatttt agctggtaca cccgatgggc atcccttgta caccccttgt
     1381 acacggctta aagataaggt aaagctaagg catctgggtt atggctgcgg atgcctggaa
     1441 aggtcgaaac gatgagaaag ggcggggtgg cgagtaataa gttcagtaaa agcaattagt
     1501 tataaatctg aggctggcga gaaggggttt atcaatggcg gaagggcgtt tgtatggctt
     1561 ttggcttcgc gaatgcctta tcggaaacgt tgacgtggta ttgtccaatc ttgcgccctc
     1621 gtttcactgg ttgcaaggcg ggcctgaagc cttgtcaaat cagatagtta atgatccaag
     1681 gccgatgtgt gcggagcagg gcaccggccg ggaaaaggat tctttgggca aaggcctgcc
     1741 caggggaaga atctatttaa gtagctacta ccgaatgact aacggtttat ttccgtttgc
     1801 ttcgagcgac agtcgtgacg ttatgccgga gagggttctt cggttttgta aggttcaata
     1861 gcgtcgaggc ggtggcggtg ggcgacctgg tggacttcga attcgacttc gtcgccgact
     1921 ttgtgcccga tcaacgcctg ggccacgggg gagaggtagc tgacgatatt cctttccggg
     1981 tcagagtccc aggcgccgag aatggtaaag cgttccgact ggttggtggc caggtcggtt
     2041 gcatatacca cggtgccggc tccggcgacg tccgtgcgca cgccagcgaa gtcggtgccg
     2101 cgcgcgcgca ccagttccgc ttccaggtcg gctttgcgcc gcaggaggag tttctgcatt
     2161 tccttggcgg ctttgtattc gtggttttcg ctgaggtcgc cgtagctgcg ggcgatggcg
     2221 atttcccggg aattggcggg gatcttcttt tggaccagtt cgtgatattc gtgtttgcgc
     2281 cgttcgaggc tttcccagga gaccaccagt gtggattcct gccgggttgg ttcgccggag
     2341 atcagggatt gcatcgtggg gtagcttttg acgatgcgcg ccagcagcga ccgtttgtcc
     2401 atgtcgtcaa acgaggggga aagctgcagc gcccgggtga ggtccttgat gacttcgaag
     2461 tccgccgagc tgatgagctc caggaggagg ctggggtcgt ccacgatgaa gtcccgcagc
     2521 cggttggagc gccgttcgtt gaactggtcg cgctccagag cggtgagcat ggcacggaag
     2581 acttccggtc ccaggatgtc ggcgaaggag tcgttgcgtt ctttggccag ccagaggagc
     2641 aattcgctgc tggcgctgtg ctggctgatc aggcgcgcta gggtttcctt gagttcttcg
     2701 aggtggttat tttgaatcag gaagccggcc agttcgccgc aggttcgggc cgagacggta
     2761 ttgaacgcca gttgcagcac gtccagccag cggtcgggga agttggtttt gaaggattcc
     2821 agcgcccggc ggcggcgggc gttggggagt tgctccagca gcaggcccgg ctcggacgcc
     2881 tgcgtccaga cggtctgggg ggtgctttcc tccgcggcgg gctgcaggcc gatggccgcc
     2941 cggacgtcgt cccggacaaa gatggcttcg agggccacgg cgggctgggt gcgcaagtga
     3001 ctggcgattt cggtgttgag cagggcgacg atttccgcgc cggccgccgc cgggtcggtt
     3061 aaatcgtcca catttttgat cacctcgagg gcggtggaac tgtgggcttt caggcctttg
     3121 gcggttcgga aagcggtcat cagccgttgt tgcggcgaga tttcctcgtc ctcgtagatg
     3181 atgggctcgg attttttgag gggcacgtgg aaatggccgt cctttttgag ttcgcggcgg
     3241 gcagtttccc accatttggg ccaatcggag gtgacgattt cgggtaccag cgctttttga
     3301 atctgctcca cggtggcttt gccatcgagg ctggtcagca ccagtttgac gagttccagg
     3361 tgctgggtgg cggccatttt gcgcagattt tccagttccg aggctttccg ggcgaggata
     3421 tggtctttgg ggatgggttt gagcgattcg gcggcgaagg ccaggtccat ggagtggtcg
     3481 ggcttattgt cgaaatcaat cgtgaagcgc gcaaagacgg ggtccagggt cttgatgcgt
     3541 ccaaatcccc agcttcggtg caggcagaag ccggtgtcgg tgagttgcac cagggcgggg
     3601 atgaggtgcc gttccagttt gcccgctgcc gccatttttt cgaattcttc gcgcatataa
     3661 tatcttaaca ctcgcttccg gtgaggagaa tccatttatt aaacgaagtg cgcgcggaaa
     3721 aaccaagaga aatagctttg cgccttctgc agcgggaggc cgggggagat tttattgaga
     3781 atcggctggc cgcggcgctg gcccgggggc ggttgtcggc cgccgaccgt catctttgcc
     3841 aggagctggc ttatggtgtc gtccgctggc aggcggcgct ggattggctg atcgcgcgca
     3901 agacggatgc ccgccagctc aagcccgctt tgcgcgccct gctgcggttg gggctttacc
     3961 agattttctg gctcgaacgc atccctcccc atgcggccgt ccacgagacc gtcgcgctgg
     4021 cccgggccag ccgcttgcac gcccaaaccg ggttcatcaa tgcggtcctg cgcggttatt
     4081 tgcgcgagca ggaggccacc cggcgcctgc tggcggagct gaaaacccgg cagccgcatc
     4141 ttgggtattc gcatccggaa tggctggtga cgcgctggcg ggagcgttgg gggccggagc
     4201 cggcggcaaa gctgatggac tggaacaaca cgccgccgag ggtctttgcc cgggtcaaca
     4261 cgctgaagac cgacgcggac cggcttcttg cccagtggcg cgccgaggag gtggagtatg
     4321 gcctggtccg ccgggattgg ctggcggaaa acctggtttt cgagctgcgc gcgcatccgc
     4381 cgctgagccg gctgcccagc tttcagcagg ggatgtttta cgtgcaggac cccagcaccc
     4441 tgctggcggt gagcgaactg ggggcgcaac ccggcgagcg cgttcttgat ctttgcgccg
     4501 cgcccggcgg caagctgacc gccctggccc aggcgatgcg caacgaaggg cgcctgttcg
     4561 cccacgacac ctcccccgaa cggctggatt tgatccgcca gaactgcgcg cgcctgggcg
     4621 tttcctgcgt gcagccgcag acgcccgccg acctcgaggc gtccggcgcc gctgccgggt
     4681 ttgaccgcat cctgctggat gcgccgtgct ccaataccgg cgtgatgcgg cgccgggtgg
     4741 agttgcgctg gcgggtgcag ccggaggaga tagaccggct gcaggcgcag cagcgggaac
     4801 tgctgcgcca ggccgctgcg tggttgaagc ccggcggcgc gctggtttat agcacttgca
     4861 gcctggagcc ggaggagaac gcgggcgtcc tgcaggagtt tttgcgccag catcccgggt
     4921 tcaagctgga gcggacgcgc gagctgctgc cgtttgtgga cggcgtggac ggcagctatg
     4981 tggcgcggat aagccgcggt tcctagcccg cgcttgaaaa cccgcgccgg ggagagaggg
     5041 cgttcctccg cgcttttcgg ccagctcccg gttcctgcgc cggattaaac ccgtcgtctc
     5101 gcgcgttttc tcgctccggc gaggggtttt cggcggcggg agcggccctg ccggcagggg
     5161 cggaaagcgt tgtaatgcgg gggccgcggg gcggggttgg aacttcgcac aaaaaatgga
     5221 aagggtttag caaggcatgt tgagaacttc ggacgggatt agcgttaagt gccgtggcat
     5281 agagacccaa tagcgtcagt gcattgctgg tatgagcaaa atatcggaca tagcaggatg
     5341 gttgaccagg ggtcggtctg gggggccggc gtccgggaaa tcctgtttat tccgggtgcg
     5401 gatggccacg aagctggtgc tgagttttct gctgatcatt ttgctggtga gcggcgtttt
     5461 tgggctggtg ggggcgcggg tgatcgggaa ccgggtcgtc gcggaggcgc aggccaaggt
     5521 gcatacggat ttgaatgcgg cgcgggagat ctacctgaac aaactggcgg cggtgaacga
     5581 cgtggtgcgc tttacggcgg accgggtgct gctgctgaac tcgttgctgt cgggcgacac
     5641 gcggccggcg tgggaggcgc tggtgcggac gcgggagcgc gaaaagctgg acgtgctggg
     5701 ggtgacggat cggaacgggc gggtggtgct gcgcgcgacc tccggcagcg tggtgggcga
     5761 tgaccagtcg caggacgaac tggtgcgcgc ggtgatggag cgggcggaac cggtggcggc
     5821 ggcctgcctt gtctcggccg agaacctgcg gcgggagtcg ccggcgctgg cggagcaggc
     5881 gcatttccag tttatcgaga ccccgaaggc gcgggcgcgc gcggcgacgg aggaatcgtc
     5941 cggcatgatg ttgaaggcgg ccgcgccggt gctggattcg tcgcaccggg tgctggggat
     6001 tgtatatggg ggggtgttgc tgaaccgcaa ctgtgagatt gtggaccgca tcaaacagac
     6061 cgtatttcag aacctgcaat acaagggcaa agacatgggg acggcgacga tattccagga
     6121 tgacctgcgg atctccacca acgtgaaaaa tgaggacggc acccgcgccc tgggcacccg
     6181 ggtggcggag gatgtctatg agcaggtggt gcggcacggg cggccgtgga ttgggcgggc
     6241 ttttgtggtg aatcattggt acatcacggc gtatgagccg attcgggatc tgacgggcag
     6301 aatcatcggg atgctgtatg tcgggttgct ggagcagaaa tatgtggatt tgcggcatcg
     6361 ggcggtgatg gtgtttctgg ccgtgacggt ggtgggcgcg ctgatcgcgc tgggcctggc
     6421 gtccttcatt tccggcaaga tttcggtttc gatcaagaaa ttggcggtgg cggcggagca
     6481 ggtggcgcac gggaacctgg acacgcgggt tgagatccgt tcgcatgacg agctgctgga
     6541 gctggcggac gcgttcaact tcatggcggc ggcgctgaaa aaacgggacg aaaaggtgcg
     6601 ggaattcacg acgcggcgga tcatgcagtc ggagcggctg gcccatatcg ggcaattggc
     6661 ggcgggggtg gcgcatgaga tcaataatcc gctgaccggc atcgtgacct attcgcattt
     6721 gctgctggaa cgggcgtcgg cggacgacgg aacgcgggcc tcgctggaga agatcgtcaa
     6781 gcaggccgac cggtgccggg atattatccg cgggctgctg gacttttcgc gccagcggaa
     6841 acccgagaaa cggcagtcga acgtgaaccg ggtgctggag gaatgtattt cgttggttga
     6901 caatcaagtg ctgttccaca acatcaggat tgtgcaacat ctgagcgcga atctgccgcc
     6961 ggtcttcatg gacccgtcgc aaattcagca ggtgtttatg aatatgattc tgaacgcggc
     7021 ggaagcgatg catggtgggg ggcggctgac gctcaccacc cggcacgtgc cggcggaaca
     7081 ggccgtcgag gtggagttcg ccgacacggg ccacggcatc aaggaggcgg acctggaccg
     7141 gatcttcagc ccgttcttct cgaccaaaga ggtggggcat gggacgggcc tcgggctggc
     7201 catcagctac ggaattgtca aggagcacaa gggcaccatc accgtggaga gcgaggaggg
     7261 gcgcggcgcc acgttcacaa tttgcttgcc ggtcagcaac gaagaggaga acctgccacg
     7321 tgccgcttaa gcataccatc ctggtgattg acgacgagga agtggtgctc gatgcctgca
     7381 cgcagattct ggacagccgc gattgccgcg tcgcgctggc caacaatggc gcggaaggtt
     7441 tgaagcggct ggaggagctg cagccggacc tggtttttct ggatctcaag atgcccgggc
     7501 tttccgggct ggaagtgctg gagcgcatcc gggccaagct gccgctgatg gtggtgattg
     7561 tgatcacggg ctacgccact gtgagctcgg cggtcgaggc catgaagaag ggggcgtttg
     7621 attttctgcc caaaccgttc acgcccgatg aattgcgcct gattgcccgg cgcgggctgg
     7681 agcacagccg gctggtgcag caaaccaccg ccctgcggcg ggagaaggaa atgctgcggg
     7741 agaactttgc cgccattgtc tcccatgaac tcaaatcgcc gctgggcgcc ctccagcaga
     7801 acttgtacgc gctggccgcc gaactggaag acaaactggc ggaggagcag cagcagcggt
     7861 ttgcccgcct gcaggcgcgg gtggacgact tgctgaagct gattcattcg tggttgcggg
     7921 tctattcggt ggacatcaac aagctgaagg agaccttcca gccggtggcc ctggccgaca
     7981 tcgtggcccg ggcggtggaa tacacccagc cgcacgcgac gcgcaaaggg ctgcagatcg
     8041 aaaccacggt cgcggaaggg atgccgtccg tcattggcga cgcgggcagc ctgaccgagg
     8101 tggtgacgaa cctgttgggg aacgcggtca aatacagctt cccgaaccac aagatccgcg
     8161 tgcgggtcga aacccggaac gccgaggcgc tgattgcggt cgcggacacg ggcgtgggca
     8221 tcgcgaagga ggatgtgccg cggcttttcc aggattttgc gcggggccag gcgcaacccg
     8281 aaggcgcgac cggctgcggg atggggctgg ccatcagccg gcggattgcc gaggtccacg
     8341 ggggcaccat caccgtggaa agcgagccgg gccagggcag cacttttacg atttacctgc
     8401 cgttggggca gggcgtggtc caatcgagtc caactccagt ataaaggttt aaatatgagc
     8461 gaagctaaac aactcctgat tattgatgat gacccggatt ttgtggcggg cattaagtcc
     8521 attctggtaa aagccaagta cgcggtggat gtagtccata acccgaagga cgggctgcaa
     8581 gccctgaaaa ccaagaacta tgatctgctg ctgctggacg tcatgatggg gcgcggcgcg
     8641 gaggggatta tgatcgcccg caagctgggg aaagacccga aattgcgcaa gttgccggtg
     8701 ctgatcatca ccgggatccg cgagcagatg gcgtttctat tccccggcca ggcggtgcat
     8761 cccagctttg tggaggtgga tgagctggtc gaaaagccgg tggacccgaa acttctgctg
     8821 gataaggtgg cgagcctgct caagactgcc gaaacgcgaa aggccaaaag caaatgagca
     8881 cagcaactgc ggtagcggaa cccgccgccc ccagcgcggt gccgccggaa aacccgatgt
     8941 cctttgtcga ggaaatcaac cacttgctgt acgcctcgaa gggcaatccg atcaatacct
     9001 gcatccaatg cgggacctgc tcggctacct gcccggtggc ctcgggcggg ttcatggaca
     9061 attcgccgcg gcgcatcatc gccctgatcc gggccggctt caaggaacag gtgctcaagg
     9121 ccaatacgtt ctggtattgc gcctcctgct accagtgcac cgtgcggtgc ccgcggaata
     9181 tagatatcgc ggagctgatg tacggattaa agcgctacac cgtgtggaaa accgaacatc
     9241 aggaggggct gattgggccg gtgttctcgg agacctttgt gaagatgatc ctgcgcacgg
     9301 ggcggtctta tgagccggtg ctggccccga cgtatatgtt cacctacggc ctgccggaaa
     9361 tgatccagga ggccaccatg ggcacggcgc tgatgctcaa aggccggttg ccgctgctgc
     9421 cggcgcgcat taagcggctg gataacttta agcgcatgat caagcatatc atccccctgg
     9481 gagacaaaga atgaagagtt acgcatattt ccccggttgc tcgctggaga agatggcgca
     9541 cagctatcat atttcagcgt tggaaacgtc gcgggcgctg ggcgtcgaac tgcgggagct
     9601 ggaagactgg aactgctgcg gcgcgacggc ttatttccac gtggacgaac tgctcgccta
     9661 cacgctttgc gcgcggaacc tggcgatggc ggagcagcaa aagctgccga tggtggcccc
     9721 gtgcagcggg tgctacaaga atatgtattt cacgcgcgag catctgcggc acgacgcgga
     9781 cctggccgag catatcaacg cggcgctggc cgaggacaac ctcagcttct cgggcgagtc
     9841 gaaggtgcat cacctgatcg aggtgtttgt ccatgacgtg gggctggcgc aagtcaaggc
     9901 cagcgtcaaa aaccccctga aggggctgcg ggtggcgccc tactacggct gccagattct
     9961 gcgcccgcgc aagcaggcca gggaaaacgt cgagcaaccg acgttcttcg aggatctggt
    10021 ggcggcgtgc ggggccgagc cggtgccgta cccgctgcgg ctgacgtgct gcggcggcgc
    10081 gctgatcatt acgaaccgcg aggcggcgct gagcatggtg cggaacctgc tcgaatccgc
    10141 cattcaggcg cgcgcggcgg tgatcgtgac cgcctgcccg ctctgccaag tgaacctcga
    10201 gtgctaccaa aagcaggtca acaaggagtt cggcacggat tacaagatgc cggtgatgta
    10261 tttcacccag ttaatgggcc tggcgctggg cattccgccc aagcggctgg gcatcggctc
    10321 ggaactggtg gcggtcatgc cggccatccg cgaggcgcag gaagccgcgc cggctctcgc
    10381 cgcgaaatga acctttaacc ctttatgaac ctctacgtcc tactcaaaat ggtgccggat
    10441 acggtggaag agctcgaagt cgccgccgac ggcaagtcgc tggattcaga attcctccgg
    10501 ttcaaactca gtgaaccgga cgatcacgcc ctcgagcagg cgctgatcct gaaagaaaaa
    10561 atgggcggca aggtgacggt cgtcgctttt gacgcgccgg aagtggacga tgtattgttt
    10621 acggcgctgg ccaagggggc tgaccgtgcg gtgaaaattc cgcttaacca agccagcctg
    10681 ggcacggtgg cgtccgccaa agtcctcacg gagttctttg cggccgccgg ggcgctgact
    10741 ccggattcgc tgctgttgcc cggctcgcag gcgatcgacg atctggaggg cgaactggcc
    10801 ccgttgctcg cggcggcgct gaaactgccc tatatcgggg tggtctgcgg cgtgacgggt
    10861 gatgccggga aggtgacggt cttgaaagag tttgccggcg gcttgcgcgg cgagttcgag
    10921 gtgacgctgc cggccgtgct gggaattcaa tccgccgaaa agccgccgcg ctacgtgccg
    10981 gtggccaaag tgcgggccgt gatgaagtcc gcccaaatcg aggaggcggc cttggcatcg
    11041 cccgagcagc cgatccagct tgatattgaa cgaatgtaca agccggaggc tgcgggccgc
    11101 gcgcaaatga ttgaaggatc caccgaagag gtcgcggacc aattggtgga agtgctggca
    11161 aaaaacagtg tgatctaagg agactcagtt atggcaaatc caatactcgt tctggccgag
    11221 caactccggg gcgaaatctc ggacatcacc tttgaaatgc tgggggttgg gcgcgcgttg
    11281 gccagcgccg tgggcgcccc gctgtatgcg gtcttgctgg gcagcgaagc cgcgccgctt
    11341 gccgcgaaac tgggaaaggc ggacaaagta ttcgtggcgg ataatccaca acttaatctg
    11401 gcgcccgccg ccaccgtcac ggcggtgctc aaggcggtga ttgaccaaag ccaggcgggc
    11461 ctggttttgg tcggatgcac caacgtgtcg ctggggattg gctcgacgct cgcagcgcag
    11521 acaggtttgc cgctggtgaa cttctgtcgc gcggcccggg ccgacggctc cgccattcag
    11581 ctcaccagcc agttgttcgg cggcaagatc ctgtcggatg tgaagctgcc ggacgggcgc
    11641 ggcatcgcct gcctctaccc gggggcgttt cccgccgacg acggcaagag cgaccagacg
    11701 ccgccggtcg agaagctgga cccgcccatg gaaacgccca aggtgacgtt caagcaatac
    11761 atcgagccgg aaaccggcga tgtggacatt acgaaacaga atatcctcgt gggggtaggg
    11821 cgggggattc agagccagga caacgtgcaa ctggccgagg atctcgcgca agtgctgggc
    11881 ggggccgttt gcgcttcccg cccggtgatc gaccagggtt ggcagccgtt gagccggcag
    11941 gtcggcaagt ccggcatgac cgtcaagccg cgtgtttatt tcgcgctcgg catcagcggc
    12001 gcgccggaac actgggaagg catgcaaaac tcgcaatgca tcatcgccgt caataccgac
    12061 cccaaagcgc cgattttcga cggcgcgcac attggcgtgg tcggcgacgt gctggaattg
    12121 ctgccggtcc tgacggacaa agttaaagcg cgcaagggat aaaatgagcg aagcaccgtc
    12181 agccacccat ctttcgggaa taacgcaaaa cccgcggcca aactgccttg accgaaaggg
    12241 tttgggtttc gagttttggg ctgcttcggt gttccggttt tatgggatga attcgcgggg
    12301 cgccgtcccg cgcataaccg agtaatgccc tatggatcca gttaaacttc ctgtatttgg
    12361 tttgccgggc ctctggttgc tgtggctgct tacgcttgtg gcctttggtg tttttggccg
    12421 ccgagcctgg cagctcattg ggttattgcg gcagggacgc tacgaaaacc gcttcgacca
    12481 cccggtccgg cgggttctgc acgccgtgcg ccacgtgctc tggcagccgc gcatcttcaa
    12541 cgaacggagc attggcctgc cccatttcct tatcttttgg ggctttgtcg tttatgcgct
    12601 ctgcttcaac tggagcctgg tgcgcgggct gtttccgttc ctccccgttc cgtacccgga
    12661 cgaggtcaat ctcaccagct tctttctgga ggtattctcc gtgctggtgc tgctctcgct
    12721 cggcgtggcg atcgtgcggc gcctgttctt cgcgccgaaa tacctgcatc tgagcctgga
    12781 tgccaacctg attctggggc tgatcgggct gctcatgtgc agcatggtct tcttctttgc
    12841 cttccgcatg gtgcatgaag gcggggcggc gtctgcctgg ctgcctttca gcagccagtt
    12901 ggcgcgcgct tttgccggcc tgtcgccgga gaccgccggc accctgaccc aggccatgtg
    12961 gtggctgcat atgattgtgg tgctgttttt cctggtgtat ttgccgtatt ccaagcactt
    13021 gcacctgctg gcctcgcctt tcaatgtgtt tttcagccag gccgccaccc gcccggtcgg
    13081 cgatctcggc gtgctcgacg ccaaagacga tttgacgtcc ggcgcttcgc gctggcagga
    13141 gcttacttgg aaacaactgc tcagcgggtt ctcctgcgcc gaatgcggac ggtgtgaccg
    13201 ctcgtgcccg gccacggcct ccggctatcc gctccagccc cagcaaatca tccagaagat
    13261 caaggaccac atggtccata ccgctctgaa tggcggaaac ggcgaaggcg cgcccaagct
    13321 catcggcgat gtgatcaccg aggctgaaat ctgggcctgc accacctgca tggcgtgcat
    13381 gaattgctgc gccgtctgga atgaacaggt gcccctgatc gtcaccttgc ggcggcatct
    13441 tgtcagccag ggtgaggttg agcgcaccgt gcaggatatg ctcgccaatc tgcagcgcta
    13501 cggcaattcc tttggtaaat ccgaccgtat gcgcgccaaa tggacgcaaa cctccgggtt
    13561 gaagatcaag gatgcccgca aggaagccgt cgaatacctg tggttcgtcg gcgattacgc
    13621 caactttgac ccgcgctgcg aagatatcac ccggcagacg gccaacgttt tcgctcgagc
    13681 gggcgtggat tttggcctgc tctacgaggc ggagcgcaac gccggcaacg atgtgcgccg
    13741 cgtcggggaa gaagggttat tcgaaatgct gcgggataaa aatctccagg cgctgggcaa
    13801 ggcgcagttc cggaaaatcg tcaccaccga tccccatacc taccacacgc tcaagaacga
    13861 gtatccctgg aacggccatc gcccggaagt ctggcattac acggaagtgc tggcgcagtt
    13921 gctggaggcg ggccggctgc cgctgcagaa gaaactttcc ggccgcgtga cctatcacga
    13981 cccctgctac ctcggccgct acaatggcgt ttacgaggct ccccgccgcg tgcttcgcgc
    14041 gttgggcgtg gagttggtcg aaatgccgcg ctgccgcgac aagagcttct gctgtggcgc
    14101 cggcggcggc cgcatctgga tggaagacac ggagaaaatc gctgagcgtc cggccgaaaa
    14161 ccgcatccgc gaagctgcgg ctctggcggg cgtgacaacc tttgtcgttg cctgcccgaa
    14221 ggacatcgcc atgttccgcg atgcggtcaa gacggcgggc caggaaggca agatcgttgt
    14281 caaagacctg gccgaactgg tcgcggaggc catggccgag ccccggccgg aggcccccag
    14341 cccgaaacct gaaaccaatc cgaaacctga gtcccccaaa tgaacatgaa aggatcgcaa
    14401 tggtcgctgt gttagaaccg gcagttgttg ataaaaccac cacgctggag acgctgctcc
    14461 agacgccggc gggccggcgc atgatgagct gcatccaatg cggcacctgc ggcggcacct
    14521 gcccctacgg cgagcacatg gagttttcgc cccggcgcgt cattggcatg ctgcgggaag
    14581 gctttctgga cgaagtgatc aaatccgaca gcctgctgtg ctgcgtcgcc tgctatgcct
    14641 gcatggccaa atgtccgcgc ggcatccggt tgacggacat cctgctcccg ctggtcaagg
    14701 aacaggtgct tatcacccgc tccgaaatgc cggcggaatt ccagaaatca ctggagaaca
    14761 tacatcgcta cggcaacccg atgggcgaat ccccgcgcaa acgcgccgcc tggactgcca
    14821 ccgccggaac aaagattccc gtgctggccg aaatcaagcg cgaggtggac gtgctctggt
    14881 ttgtggagtg ttatacctcc ttctacgccc gcgggcagga taacagccgg gccaccgcca
    14941 aactattcca ggcgctcggg gtggatttcg ccatcctagg gaacgaggag aagtgcgcgg
    15001 gtgactgcgg ccagatgacc tgggaatcgg ggctgttcga atccctggcc gaatacaacg
    15061 tcaagctgtt caataaatac aaattccggc gcctggtgac cggcgatgcg catgcctaca
    15121 atgcgttccg cttccgatac ccgatgtatg gcatgaccca gccggccgag cataccacgc
    15181 aattctttgc gcgccacctg gaccagctca agccccggct caagaaaccc ctcaatctca
    15241 ccgtcactta tcacgaccca tgctgcctgg gccgggccga caatatctac gacgagccgc
    15301 gggcgctttt gcgggcgatt cccggtataa aactggtcga gatggttcac caccgcgtca
    15361 atgccgtctg ctgcggcggg ggcggcggcg gcatgtggct ggatacctac tacaagcagc
    15421 agggcatgga tcggctgtcg gagcggcggg tcaaggaagc cgttgccacg ggggccgatg
    15481 tgctcgcggt ggcctgtccc tacgaagttt cgcggtttga ggacgccctc aaagtggttc
    15541 cgcacaataa acctatggtg gtccgggacg ttgtcgaact cctggccgag tccctggaat
    15601 agaaagctta gcattttatg aaacccattg taccgaagaa gaacgaaccc attcgcatcg
    15661 gctattatat ctgccattgc ggcacgaata tcgccggcat ggtggacgtc gaggcggtcg
    15721 ccaaatacgt cgccaccttg cccaacgtag ccatttcgcg gaattacaaa tatatgtgct
    15781 ccgacccggg gcaggagttg atccagcagg acatccgcga gcaccggcta aaccgcatcg
    15841 tggtcgaggc ctgctcgccg ctgttgcacg aactcacctt ccgcaaagcc acggaagccg
    15901 ccgggctcaa tccgttctat gtccagatgg tcaacacccg ggagcataat tcctgggtgc
    15961 ataccgatcg gaccgaggcc acccgcaagg ctatggccct ggcgcgcgcc gccatccagc
    16021 gcgtcccgca ccataaaccg ctcgaagtga aaaaggtccc ggtcaacccc aatgtcctga
    16081 tcgttggcgg cggcatcgcc ggcatccacg ccgcgcttac cctggccaat gcaggcaaga
    16141 tggtctatct ggtggacaag gaggcgacca ttggcggcca catggccatg ttcgacaaga
    16201 ccttccccac cctggactgc gccatgtgcg tgctgtcgcc caagatggcc gccgtaggct
    16261 cgcaccccaa catccatctc tggacctact cggaagtggc caaggtggac ggctatgtcg
    16321 gcaatttcaa cattaccgtc cggcgcaagc cgcgctacat tctcgaagac ctctgcaccg
    16381 gctgccagga atgcgtggat gcctgcgtct tcaaagagcc gaagttcccc gacaagttca
    16441 atctcggcct gggcaagcgc aagccggtat tcctcccgtt tgcgcaggcc attccgcccg
    16501 tggtgatgat tgaccccgag gtctgcctca acttcaagcg cggcaagaat cccgacggca
    16561 cccataccgt ctcggacaaa tgcaaaaaga cctgcgtcca ggcctgcggc gatcgcaaag
    16621 cgattgactt caagcagacc gaggaattca aaaacatcac ggtgggcacc atcatcatgg
    16681 ctacgggctt ccagatattt gacgccaaac gcaccccctt ctatggctac ggcgtctatc
    16741 ccaacgtcta taacgccctg gaaatcgaac ggatcatcaa cgcctccggc cccaccgagg
    16801 gcgaactcat catgcggaat ggccagcggc ccaaaaaagt ggcgatcgtc cactgcgtcg
    16861 gctcgcgtga cgaaaacacc aaccgctggt gctcgcgggt ttgctgcctc tactccctca
    16921 aactggcgca cctcatcaag gagcacacca acgccgaggt gtataacttc tacattgata
    16981 tgcggacgcc cggcaagagc atggaggagt tctacaaccg gattgccgag gaagggatgc
    17041 acctgatccg gggcaaggtg gcggatgttt accccgaccc gaacaacggc gccaccggcc
    17101 gcctggtcgt ccaggcggag gacacgctga ccaataagat tctcaagatc ccggtggaca
    17161 tggtcgtgct gggggtgggg ctggagccgc agccggattc cgaaaacctg cgccgccagt
    17221 ttaacctgag ctgcagcagc gaaggctggt ttttggagcg gcacccgaaa ctggcccccg
    17281 tcaacacctt caccgatggc atcttcctcg ccggttgctg ccaggggccc aaagacatcc
    17341 ccgattccgt ggcccaggcc ggcgccgcgg cctccgaggc gttcgcgctg attgacaaag
    17401 gccacgtcga gcaggagccc aacaccgcct acatcctggc cgacgagtgc agcggctgca
    17461 aatcctgcat cccgctgtgc ccctacacgg ccatcacctt tgacgaggag cgcaagaaag
    17521 ccatcatcaa cgaggcgctc tgcaagggct gcggcacctg cgtcgcggcg tgcccgagcg
    17581 gctcaattgt gcaaaacctg ttcgaagacg aagaaatctt cagtgaaatt gacggcgtgc
    17641 tggcgccggt tccggtttaa ccgcccgccg agaaaggatt aaaaaatgac gaaccaaacc
    17701 aaaccctccg cttctggaga aacctggacg ccacgcattg tggccttctt ttgcaactgg
    17761 tgcacctaca ccgcggccga tctggccggc gtgtcccgca tgaaacatgc ggccaacgcg
    17821 cgggtgattc ggctgatgtg ctcgggccgg gtggatccgc agttcatcgt tgaggcgctg
    17881 gccaaaggcg ccgatggcgt gctcatcggc ggctgccatc ccaatgattg ccactacgtc
    17941 gaaggcaact acaaatgcct ccgccgctac cagatgctca aacgcatgat cgccgacatg
    18001 ggaatcgagc ccgaacgcct gcggctggaa tggatttccg ccgccgaggg cgaaaaagtc
    18061 aaacgcgtca tcaacgagat gaccgaaacc atcacccggc tcgggcccct gggcatcccg
    18121 cagaagttcg acgagtggga ccgggaaatg aaacacttcg ccgaacacgt cgccgccagg
    18181 gaagccgccg ccggcagcgc ctgcgccgaa catggcggca aacctgaaag tctggagcct
    18241 gctcatgcct aagcctaaaa ttgcttttta ctggtgcgcc tcgtgcggag gctgcgaaga
    18301 aaccgtcgtg gacctcgccg aggacatcct cggcgtagtc gaaaaagtgg acattgtcct
    18361 ctggcccgtg gcgatggatt tcaaatacaa ggacatcgag gccatgcccg acaagtccat
    18421 cgccgccacg ctgctcaacg gcgccatccg ctccgacgag caggaggaaa tggctcacct
    18481 gctgcgccgc aagagccagt atatgattgc ctatggcgcc tgcgctcagc tcggcggcat
    18541 ccccggcctg gccaaccagt tcccccgcga acaactcctc aagttcaatt acgaggacgc
    18601 tccgaccatg gtcaacgaca agaagacccg cccccaactc acgaccagcg acaatggccg
    18661 cgagccgacg ctccccgagc tccacaatgt cgtccgctcg ctcgaccagg tggtcgaggt
    18721 ggattactac gtgcccggct gcccgcccac gcccaaggtc acccggacgg ccctcaccgc
    18781 cctgctggag aaccggctgc cgccaaaagg caccgtgatc gcgccggaca tcgcgctgtg
    18841 cgatgaatgc ccgcgcaagg ccaccaagcc cgccaatatc gcgttcaccg agttcaaacg
    18901 gccccaccag gcgttgatgg acccgaacct gtgcttcctc gcccagggcg tcgtttgcct
    18961 gggccccgcc acccgcagcg gctgcaacgc ggcctgcacc gtcggcggca tgccatgcac
    19021 cggctgcttc ggccccacct cccgcgtacg cgatcaaggc cttaaaatcc tgtcctcgat
    19081 ctgctccaac atcgccccca aagaagaggg cgaaatcgaa aaagtaattg aaggcatccc
    19141 cgatccggtg ggcaccttct atcggtacgg cctggccaag agcctgttgc gccgcaaagc
    19201 caccctcccc tcaaagtaaa agagatccat atggaccaaa ccgttaaaga gacattcaac
    19261 cagggcgccg cccgcgccaa cgcggcctat caaatgggcc gccgccgctt caccatcgag
    19321 cccatcacgc gcctcgaagg ccacggcaag atcgatatct ttctaaacga caaaggcgat
    19381 gtcgagcggg cctatatgca ggtccccgaa ttgcgcggct tcgaggtttt cagcctcggg
    19441 cgccccgccg aggacatgcc gcaaattacc agccgcatct gcggcgtctg ccccacctcg
    19501 caccacatgg cttcgaccaa atgcctcgac gatctttacc aggtcgagcc gaccgtgccc
    19561 ggccggaaga ttcgcgagct ggtctataac gccttcattc tcgaagacca tgccctgcac
    19621 gtctttgtgc tgggcgggcc ggatttcatc gtcggcccgg aagccccccc ggccgaacgc
    19681 aatatcgtcg gcgtgatcgg caaggtgggg ctggaggcgg gcaagaaagt cattgcctcc
    19741 cgccgccgcg tgcgcgagct catcgcctat ttcggcggca aggtggtgca tccggtgctg
    19801 gggttgcccg gcggcgtcag caaggcgctg gacccggccg acctgccccg gttcaaggaa
    19861 ttggcccggg acgcggtcga gtttgccgaa tggacgctcg ccgtgctcaa gcaaatcgtc
    19921 ctggagaacc ccgaatactt gaagatggtc accagcgacg cctataccca caagacctgc
    19981 tatatgggca tggtggacga acagaatcgc gtcaacttct atgacggcaa gctccgcgtg
    20041 gtggactgca acggcaagga aatctgcaaa ttcccgaccc ggcagtacct cgattatgtc
    20101 gccgaacacg tcgagccctg gagctacatg aagttcacct acctcaaaca gcgcggctgg
    20161 aaggatttcg tggaaggccc cgacaccggc atctatgccg tggccccgct ggcgcgcttg
    20221 aatgcctccg atggcatggc cactcccaag gcccaggccg cctacgagga cttttacaaa
    20281 accctcggcg gcaagccggt ccatttcacc ctggccaacc acctcgcgcg cgccattgag
    20341 atgatctatg ccgccgagcg catgcaggaa ctggtgaacg atccggacat catcaacccg
    20401 gacgtgcggc gcgttcccac gagcatcccc aaagaaggct tcggcgtggt ggaagccccg
    20461 cgcggcacgc tgttccacca ctaccggact gacgaacgcg gctgcatcac gatggccaac
    20521 ctgattgtgg ccacccagaa taacgccgcg cgcatcgcca tgagcgtgga tcgcgccgcc
    20581 aagggcctca tcaaaaacgg caaagtcacc gagggcctcc ttaacaaggt cgaaatggcc
    20641 ttccgcgcct atgacccctg cctcgggtgc gccactcact cgctccccgg ccatctgccg
    20701 ctgcgcgtca acatttacga ccggcagcgg aacctggtgg aacaactcgt gcaggactaa
    20761 cccgttcttc ggagttcaag gttccgcccg ccggcgaatc ctggattccg gattgcggag
    20821 gtgcgtcatg ctgccgcttg cgcccgaaat gaccgccgcg ccggagccgt catccgctcc
    20881 ggcccggcgc accctgctgc tggggctggg gaacgacatc ctcaccgacg acggcgtcgg
    20941 cctttacgtg gttcgccagc ttcgggccac gctggccgga tgcccggcga ttgatgtccg
    21001 tgagaccatg gagatgggat tggcgctcct ggactttatc agcggctatg agacggtcgt
    21061 gctcgtggac tccatccaga ccggccgcgt ggcgcccggc gtcgtgcatg aactggacgc
    21121 cgccgccttg cggcagctca ccgggcgcac gccccacttc gtcggcattg gcgaaaccct
    21181 tgccctgggc cggcacctgg ggctgcccat gccgcaacag gtccgcatct ttgccatcga
    21241 agtcgccgac cccttcacgc tcggcaccgc tttgtccccc gctttgcagg ccgccctgcc
    21301 cgccatcgtg gagcgggtgg gccgggccct tcggtcgctg gcgtaagccg cgcggccgcg
    21361 ggttgcccct gccgggaagg gtcaggaacg cggcgcgatc accagggtct tttccgacat
    21421 gaccgtggcg ggcagggttt caatgtctgc caggtgctgc atccaccact gttcggcgtc
    21481 gcgcccggtc caggttcccg ccgacgcgca cgccgccaga gcctgatcca gggcctccgc
    21541 gctggcgggg cgctgcgcgg cctgcttggc aaggcagcgc aggagaagtt cagccaggtc
    21601 cgccgagacg ggctggccca gccgctcggc cgggggcgga ggcgggcttg aaacctgccg
    21661 gctgaacaac ccctcgacgg tgtcggaatc aaagagcgtc ttgccggtaa gcagccaata
    21721 gccaaccgcg cccagcgaat aaaggtcgct ctggctcgtt gcgctctccg gtttttcgac
    21781 cgcctccggc gacatgaaat gcggcgtgcc cacgatatcc cggggcgccg ggccgaacgc
    21841 cgctcccgga tgcgcggccg ccaccagccc gaagtcgagc accttgacca ggtcgcaaac
    21901 gccgccgcgc cgggtcagaa tgatgttggc gggcttgacg tcgcgatgga tcaggccgat
    21961 gcggtgcgcc tcggccagcg agccgcaggc ctggcgcagg atgtgaatga cccgtccctc
    22021 gggttgcgcc ccgaaccggc gcaccaactg gtccaggtca atgccgccca ggtactccat
    22081 ggcgaaatag aacaacccct ccggggtgcg cccgtagtca taaatggcga cggtattcgg
    22141 atgggttagc tggctggtca tctgcgcctc gtgttcgaag cgggcggagt tggtttcgtt
    22201 ggtctgatcg gggttgagca gcttgatggc gaccgggcgg cgcaagagcg aatggcgggc
    22261 gcggtaaacc atgccgttgg cgccgcgtcc aatctcctgc atcagcacgt actggcccag
    22321 ccggcgggcg gtaagcgcat cctttcgccc ggcggcctgg aggtgttcga ccagcagcgt
    22381 gaagccaaaa atggcgccgc cgctggccag gagcagccca aagagcacca ggaaaacgcg
    22441 ccgcaaaacg tagagcgtct ggaacgcctc atccagcgac tgctcggcgg ccacccccat
    22501 gccgcaatcc cgcagccagg cccaggcgcc gatcaccttc aacccgcggt agttgcgata
    22561 gccgcgcacg tcgaaatcat tgtttcccat cgtggcggag gcggccatgc gggtgagatg
    22621 cagttggctg cgcggcttgg ggtgcggaac gcccgggtgc aattccccct cgggatccag
    22681 cagcttcagg ttcaggatgg cggtgttggt gcgccccggc gggatgagcc ccagcgtctt
    22741 gagcgactga tcaaagcggg tcgccgtgag catcaggccg ctgcggtcaa aggcataggc
    22801 ttccccggtc tggccgatct gcgcgacgga aaagatgctg gtgaactcct tctccggccg
    22861 catgcgcagg cccaggacgg cggccacgac gtcgttggtg gtcttgatag gggcggccac
    22921 aaacatggtc ggcccctcga cccgccggct caggttggtc tggctgacaa atggacgcga
    22981 ggtggccagt tgcccgtcca acaccttgtt aaggaacagg ttgtaagcgg tcggcgcccg
    23041 gaccccgatt tcctcgggac cggtggtggc caggatgcgc ttgtccggcg ccaccacgac
    23101 gtagccaagg tattcctggg cctccagaag cggctgcagg tagtgccgca aggtttgggc
    23161 cggcgcggaa tctgccaggg actgcggcgt ggcgcgggga tcctgcgcca gggccgccag
    23221 ttgcataatt gcggcttcga cgcgcgcatc cgacgcaaag gatttggcgg catactgccg
    23281 ctccgtgaac cacaaacgca aggcattgac gtccgcctgc agcaacgtct gcaagcgcgc
    23341 cgccagctcg ccgcgggtgg tttcttccac ccgtttgacc acccacagcc cgacaatgcc
    23401 caggatcagg gctccagcca gcggcacggc ccacaggcgc ttggacaaca gccgcggcaa
    23461 ccgaatcaag aaccggttga cggcgctcat gcgaggccct gttccgtcaa tgctcaccgc
    23521 tcaagactgg ttcgcccggc aacagttgtc aacggagacc aacaggccgg caccccccat
    23581 tggctcgtag aaccgggaaa aagagcctcg ccccccaccc gcccgcccag ccgcagccag
    23641 ttgtgtccgt caagttcagc aaattggtcg tcatggtttc cactcaaggt
//
LOCUS       DAOPQG010000057        23271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2754_length_23271_cov_14.228032, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000057 DAOPQG010000000
VERSION     DAOPQG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 23271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23271
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2754_length_23271_cov_14.228032"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1867
                     /locus_tag="PKI07_08075"
     CDS             272..1867
                     /locus_tag="PKI07_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC51050.1"
                     /translation="MRTPREAAQIILDAADALWQWDAALLGLCSHDPSQIDLVVCVDL
                     IKGRRTELPVSTIPKRLSAIARKTLSRGPQLILRPASAGFPPEALPFGDQTRPSASLM
                     YVPLRKDAQVIGLLSIQSYAPHAYNETDLDTLQALADHCGGALVRIRAEAELMESNDR
                     LRMALAAGRMGTWTRELAEPGSRFWSPELEAIFGLAPGRAPQTDQDFYELIHPEDRGV
                     VQKFFAQAAARKADHEVEFRFLPRHRPPGWMLARGRVYYDAAGKPLRLAGVAIDITAR
                     KEAELESSRLNEELEQRVRKRTAQFKAANQELEAFTYSVSHDLRAPLRGIRGFSEILL
                     ADHAHQLDAAGQELLRRVCDSSQRMGWLIEDLLELSRVGRGKLRWQPVNLSAQAGAIA
                     ASLRHDDPARTVRFIIAPNLQAAGDERLLQVLLDNLLRNAWKFTRSQPDPQIEFGFVS
                     HPEPAFFVRDNGVGFDMAHAGKLFGVFQRLHSDAEFPGTGVGLAIVQRIVQRHGGRVW
                     GAGEPRQGATFYFTLPAQRPLEL"
     gene            1864..3048
                     /locus_tag="PKI07_08080"
     CDS             1864..3048
                     /locus_tag="PKI07_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC51051.1"
                     /translation="MSQALRVLLVEDSADDAALLQYELQRAGYAPSCRRVETAETLNA
                     ALEEQAWDLVIADHRLPRLDALSALAQVKARGLDLPFIIVSGYITEEAAVATMKAGAH
                     DYVMKDKLARLGPAVARELREAVVRRERRRAEDALRQAYAELEQRVAQRTAELKTANA
                     RLESAIAERQRLEHELLEITENERRRIGLDLHDDLGQQLSGLALMTKGLERKLARRKA
                     GEAEDAARIHQLVQQTINLTRSLARDFATLDLEADDLPAALDRLAAHAAWVFKISCQL
                     EQCGPLPALGAGIARQLYKIAQEAVTNAVKHGQAKSVRIGLASRPGQIILTLRNDGAP
                     FPKLTEPATGLGLRIMNYRASLIGASLEIKRDSPAGACVICAVPLPAAESRPPPPGTP
                     AA"
     gene            3248..4703
                     /locus_tag="PKI07_08085"
                     /pseudo
     CDS             3248..4703
                     /locus_tag="PKI07_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110905.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            4700..5323
                     /locus_tag="PKI07_08090"
                     /pseudo
     CDS             4700..5323
                     /locus_tag="PKI07_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
     gene            5536..6648
                     /locus_tag="PKI07_08095"
     CDS             5536..6648
                     /locus_tag="PKI07_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOC51052.1"
                     /translation="MKKGWQIKRLGEVCEIVKGRKPMLKLTPASGDLPYLVAKVMRGS
                     AEAKYASVKDKNSITVEESETIIICDGSNSGEVFTGFRGILSSTMGKIAKKVEIDDNY
                     LRAFLASTFEVFNGTKTGAAIPHLDKEAMYQLQFPLPPLPEQRRIVALLDQTFAAIAT
                     AKANAEKNLQNARALFESHLQSVFTRRGSGWVEKTVDTLSTNLDSKRIPITKSNRKAG
                     SYPYYGASGIVDYVADYIFEGDALLVSEDGANLLMRSTPIAFSVSGKYWVNNHAHILK
                     FENMATQRFVEFYLESLKLDEYIRGAAQPKLTQKALNSIPIPIPTTFEEQARIVENLD
                     SLYKETQRFSSIYERKLAALDELKKSLLHQAFTGEL"
     gene            6838..9651
                     /locus_tag="PKI07_08100"
     CDS             6838..9651
                     /locus_tag="PKI07_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOC51053.1"
                     /translation="MNEAETRAEYVDPALKAAGWGVVEGSRLRREYPIAPGRLEGHGK
                     RGKALTADYVLEYRNTKLGVVEAKALDEELTEGVAQAKNYAGKLAVRHTYATNGRGIY
                     AIDMQTGAEGEIAAYPTPDQLWAMTFPSTRSGTGDAADRASELAELWRDRFAAIPFED
                     KGGSHPSRYYQDIAIERAMQAIARGQSRILLTLATGTGKTFIAFQIAWKLFHARWNLG
                     GQPTRRPRILFLADRNLLANQAYNAFSAFPEDAMVRIKPEDIRKKGKVPKNGSLFFTI
                     FQTFMASAGSATEKGAIPEPVEGAASELPEVAEPTEKASGYMYILGCGDGSFYTGSTT
                     NLHLRLEQHRSGEGAQHTKARLPIKLLYYEAFDRIDAAFAREKQVQGWSRAKKFALIQ
                     RNIGLLKRLSSASADGRPVPEPVEGCFGEYPPDFFDFIVIDECHRGGANDESNWRGIM
                     EYFAPAVQLGLTATPKREENADTYRYFGEPVFIYSLKEGINDGFLTPFRVKQIQTTLD
                     YYVYTPDDQVVEGEIEAGKLYDEPDFNRIIEIKEREQKRVEIFLGEINQNAKTLVFCA
                     NQAHALVVRDLVNQLKTSKDPNYCQRVTANDGELGEQYLRDFQDNEKSIPTILTTSQK
                     LSTGVDARNIRNIVLMRPINSMIEFKQIIGRGTRLFDGKDYFTIYDFVKAHLHFNDPE
                     WDGEPVEPESCPQCGCSPCVCETPPPQPCPVCGKSPCACPKAPCPVCGQLVCVCNQKS
                     KVKVKLADGKERNLRHMMATTFWHPDGTPMSARQFMERLFGKLPDFFQSEAELRNLWS
                     APDTRAKLLQGLAEKGFGRAQLAEMQKIIDAEKSDLFDVLAYVAYALPPLTRAARAAG
                     ARVYINSHFNAKQRAFLDFVLSHYVHTGIEELSREKLRPLLLIRYHNSIQDAVADLGQ
                     PAQIGNLFASFQKYLYQPGPTPA"
     gene            complement(9862..9946)
                     /locus_tag="PKI07_08105"
     tRNA            complement(9862..9946)
                     /locus_tag="PKI07_08105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Leu,seq:cag)
     gene            10141..11112
                     /gene="galE"
                     /locus_tag="PKI07_08110"
     CDS             10141..11112
                     /gene="galE"
                     /locus_tag="PKI07_08110"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOC51054.1"
                     /translation="MNIFVTGGAGYIGSICAEALLEAGYAVTVYDDLSEGHRSAVDRR
                     ARFVQGRPDEAGHIFNAIQEARPDAIMHFAANALVGESMTNPRKYFHNNVVNGLKLLD
                     AAIEAGVKKFVFSSTCATYGPPERLPMTEDLPQRPINPYGESKLMFEKVLSWYQRIYG
                     LEFIAFRYFNAAGASERFGEHHRIETHLIPNVLKVALGQKPHCEIYGTDYPTPDGTCI
                     RDYIHVRDLARAHILGLAPGRQGFYNLGNGDGYSVRQVLQTCEKVTGKTIPALEKPRR
                     PGDPPRLIASAARAMEELGWQPEHPRLEDIVASAWAWHQRHPQGYPD"
     gene            11381..12244
                     /gene="mtnP"
                     /locus_tag="PKI07_08115"
     CDS             11381..12244
                     /gene="mtnP"
                     /locus_tag="PKI07_08115"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOC51055.1"
                     /translation="MAQYRVGIIGGSGLYHIEGFTRQKWVKVKTPFGSPSDDFLTGKL
                     AGREVVFLPRHGRGHRILPSELNHRANFWALKKLGVAWVISVSAVGSLQARYRPCDIV
                     LPDQFYDRTKQSAAHSFFGRGIVGHIAFAQPICEELRQLLLQAARALKARVHDGGTYV
                     NMEGPAFSTRAESITNRKLGHDVIGMTNLGEAKCAREAEIAYATMAMITDYDCWKEEE
                     APVTVEMVIANLRKNAQTAQAIIARVLPRIPAAPNWPCHDALKNAIMTDRKCWPARTL
                     RELEPILRKYQ"
     gene            12306..12689
                     /locus_tag="PKI07_08120"
     CDS             12306..12689
                     /locus_tag="PKI07_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOC51056.1"
                     /translation="MTQKTIIKPARSAPAAGPYNHAVRVGDLLFCAGQIPIDPATGEL
                     VGGGIQAQTERVLQNIKAILDDQKLTFAQVVKSTVFLTNLADFAGMNEVYARFFTADF
                     PARSTIQVAALPKGASVEIEVVAHY"
     gene            12751..13563
                     /gene="lpxA"
                     /locus_tag="PKI07_08125"
     CDS             12751..13563
                     /gene="lpxA"
                     /locus_tag="PKI07_08125"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOC51057.1"
                     /translation="MIHPTAIIHPRAKLDPTVQVGAYAIIDADVEVGPHCVIGPYVYL
                     TGVTIIGAHNRFYAGCVIGEAPQDLKYHGELTGLRIGDHNVFREHVTVHRSNQAAEET
                     VIGARGFYMAHSHIAHNCRLGDGVILANGVLLGGHVTVEDGAFLSGNCLVHQFVRVGT
                     LALMQGGSAISKDLPPYTVARGGNRLGGLNIIGLRRAGFSPAERLELKQLYHALFRKG
                     HNLRAAAAAAREKFTSAPARRLLDFLAAARRGVCGEADEQEDKNIDEAPAGG"
     gene            13598..14650
                     /locus_tag="PKI07_08130"
     CDS             13598..14650
                     /locus_tag="PKI07_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51058.1"
                     /translation="MKWLGGAAALLLAAGLGGQAQAAGRSAGRPIQFTPARSDEVLTN
                     LHQLSNKKDSLKRLEEDLNAPLQSLAPRGALEGVPARVSRPSAPSAVQSKRARELLER
                     RKNWIFMAPEDIIGNPTVEQVLEAPEFDENGQIKKDLPAIESYYLRLLPKRPARPLPE
                     SSPVEDVFGRANKPNMREAPFSDDADLPAGIRESADALRSFVQPREGDASYFLSTPAA
                     DFSLAHRLHSKAASAEQMLEHNKRMDEYRTLVDPNWRSSSATTPGLPALSSVGMPPPS
                     ATPVNKQPADSDLASAHGLEAQMNIRNPLLGPPGLPDVNAKALGQSRPLPVETKVRQT
                     KMATTEPMYTAPRRAF"
     gene            14835..16247
                     /locus_tag="PKI07_08135"
     CDS             14835..16247
                     /locus_tag="PKI07_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOC51059.1"
                     /translation="MNQQPASSVGPLPGYITSAVPNPRANRAPWYKNTAPTYAGIFLW
                     FVFWQDAAKGGAPGGTLAQGVGWALLSLVIAALVCHFLFYLVPGLFGMKTGLPLYIVG
                     TSAFGAQGGFLMPGFLMGVLQFGWVGVNIYFSSLALSAVIPVDARFIMVVWGVLACFV
                     GLKGIQYVARVATYLPLIPLVALLWLAAATIGHVGSFKPDALIAAQTAANPAGPGALG
                     LLGVLAAMLTYVVGFFATAGAAGVDFGLNSRDEKDVSRGGLFGIALAIVVTGGLSLLV
                     VAGTYGSMKETIDAVATRQLSLSSFDLIPAVLGAATAKWVMFLLAVAAFPPACFSSFI
                     AANSFKTTLPKVNPFISVGIGTGVAIVLAITGVVAKAIAVFVIIGASFGPICGALLVD
                     YLLSGRKWSGPRAGFNPAGWLAWALGFLVGILPNLGVGLPAAPVLAFAVGAGVYFLCA
                     KLGLLTPVVPVSGALAEATR"
     gene            complement(16326..20012)
                     /gene="dnaE"
                     /locus_tag="PKI07_08140"
     CDS             complement(16326..20012)
                     /gene="dnaE"
                     /locus_tag="PKI07_08140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOC51060.1"
                     /translation="MPHAEFVHLHLHTEYSLLDGACRLDRLMKKACDLEFPALAITDH
                     GVLYGAIDFYEQARKHGLKPIIGCEVYVAPGSRFEKKTTTGGRDVYHHLVLLARDETG
                     YRNLIQLATRAHTEGYYYKPRIDKELLAEHREGLLALSGCLAGEIPEWILKDQLPRAR
                     AAVDWFKQTLGAENFYLELQQHGLPEQARVNRQLVAWSKEFGLKLVATNDVHYLERDH
                     AHAHDCLICIGTQTLLSDARRLKYADGQYYLRSAAEMAARFAEVPEAVRNTLEVAEKC
                     NLEFNFRQMHYPVFTPPEHFTREGYLRHLAAEGLRARYGIHARAVGREFVVEHVEDPA
                     RLPGAPAPAASPGGSPAAAAPAGDPALRALLERLQRELQIIEETGFVSYFLIVEDFIR
                     YGRAHGIPCSARGSAAGSLVTYLLGISNVCPIRYGLLFERFLNPERVNPPDIDIDFAD
                     DRRAEVIEYVRQKYGQDAVAQIITFGTLGAKSVVRDVARVMGLTFGEGDRLAKMIPSE
                     LKITLEEALKRSPELKQACENEEVTRELIDTARVLEDLTRNASVHAAGIVIGDQPLVN
                     LLPLKQDENGAIVTQYAMGPVGKLGLLKMDFLGLKTLTVIRNTCERVRQTRGIEVPID
                     DLPLDDPKTYDLLNKANTLGVFQLESGGMRDLCRKFQIDSLEHITALIALYRPGPMEL
                     IPEFIKRRHGEIQIEYVHPLLAPICGETYGIMIYQEQVMQAAQSLAGYSLGGADVLRR
                     AIGKKQADEMASLREPFVKGCARVNRIPAAKAGQIFDLLAKFAGYGFNKSHAAAYAVL
                     AYQTAYLKANYPVEFFCAIMTNDLADTEKLSQYIAEARGMGIEVLAPDVNESQVYFAP
                     APGTPAARPPGPDADRDPASAAAIRFGLAAIKGVGEIAVQAILKARDEGGRFRSLADL
                     CERVDARTVNRKILEALIKCGACDSLGQTRSMLCGLLDHTLARAASVIADRQRGQASL
                     FDTLEEHAVPAEPAASLPEWPEHQLLACEKELLGFYVTGHPLTPYVPILQKYALANTA
                     SIAQLPARSLTRMGGLIAAVQSGTSKKTQKPYVLATLEDLEGSVQVVCLHENCEKYRE
                     LLVPGKAILVIGEVGHGDDRPKLFPQEIMPLEEAPGRFTKQVHLRLHTAHLQPGHLEQ
                     IHELARAHRGKCPLFLCLMRPAGGMIFIEAHDQFAVAPSRQLQQAVNERFGPETYYAK
                     VDTSLPERSPRRWERRANGNGAEG"
     gene            complement(20134..21237)
                     /locus_tag="PKI07_08145"
     CDS             complement(20134..21237)
                     /locus_tag="PKI07_08145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC51061.1"
                     /translation="MDKTRILGFSLPLLLAAAIPAGSAEPAPPPPAAAPPAAADGKAS
                     ALFTNTVVARGKGVEVTRALLDDEIIRLKAQLAGRGQTLPPAQLATLDRQVLDQLIQI
                     QLIKAKATAADKAAGKALAEKRFEEAKARVGSEEAFNRQLKLMNSTGEEVLARWTDSL
                     TAENVLKRELKVNITDADAKTFYEDNPAHFEQPEMVRASHILLTTLDPKTGVEMTDEQ
                     KAAKRKQMEGLLKRARDGEDFAKLAREFSEDPGSKDKGGEYTFPRGQMVPEFETAAFG
                     LKTNQVSDIVTTRYGYHIIKLLEKIPAHKIELAKVAEEIKDNLAQQALLKQLPDYVAK
                     LRAEAGVEILDEKLKAMEPPASPEAPPARPATK"
     gene            complement(21258..22856)
                     /gene="serA"
                     /locus_tag="PKI07_08150"
     CDS             complement(21258..22856)
                     /gene="serA"
                     /locus_tag="PKI07_08150"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOC51062.1"
                     /translation="MKILVCDPVSPKGIAVLQQRPEFEVVVLPKRLPEAELLPMVKDV
                     MAMVVRSETKVTRKVIEAAPQLRVVGRAGVGVDNVDVAAATEHGVVVMNTPGGNTIST
                     AELSFAMILNLARKVPQASASMSAGQWDRKQFQGIELTGKTLGVLGLGRIGSEVARRA
                     VAFGMRVLGYDPFVTEARAQALGIELVKDLDNVYRDADFITVHMPVTEQTRGMLNAVA
                     FAKMKPKVRLVNCARGEVLVENDLLAALDSGKVAAAALDVYAAEPLPADHPFRRHPAI
                     TLTPHLGASTVEAQEKCGLEVAEVIAAYLLTGEVRNAVNLPYLDAKTYEQVKPYLVLG
                     EKLGRLLGQLAPAQVDRLYLTFGGKAQELPNTDPVTRAVLLGFLSCAAVKELNNVNVR
                     KIAATLGITVEEKRSNEPVTFNEWLHVQVFRNGQKVISAGGTFFGSPDNPRIVRVFSQ
                     PTEIVPEGVVLLLSNKDRPGMVGYLGTLLARHKVNIASMNLGRDTVGGRALTVLNLDS
                     VPPQEVLDEIQNDPDISNVKVVKL"
BASE COUNT         4248 a         7619 c         7151 g         4253 t
ORIGIN      
        1 cggacggggc cggggtcgtt ccccgccggt tcttccgcat catggtcgtg aactaaaacc
       61 ggcaaacttt gcgggcctca cggccggtag ggtgtttacc ccataggtaa ccgcatcggt
      121 cgtgctatta ttgaccctcc cgaagatgcg gtcatttccg gacactcgag cgggtgtcag
      181 tgcggggaac gccgggactc gaagcgtgcg gcgcaagcag atggagcaac gcgccgcaat
      241 gttttcccgt ctgggccaac gccttagcgc cgtgcgaaca cccagggaag cggcgcagat
      301 catcctggat gccgccgacg cgctctggca atgggacgcc gccctgctgg ggttgtgttc
      361 ccatgacccc tcccaaatag acctggtggt ctgcgttgac ttgatcaaag gccggcgcac
      421 ggagctgccg gtgtccacca tccccaaacg gctcagcgcc attgcccgga agaccctctc
      481 ccgcgggccg cagttgatcc tgcggccggc ctcggccggc tttcccccgg aggctcttcc
      541 cttcggcgac caaacccgcc cttcggcctc cctgatgtat gtgcccttgc gcaaagacgc
      601 gcaggtgatt ggcctcctgt ccatccagag ctatgcgcct catgcctata atgaaaccga
      661 cctggacacc ctgcaggcgt tggccgacca ttgcggcggc gcgctggtcc gcattcgcgc
      721 cgaggcggag ctgatggaaa gcaacgaccg gctgcgcatg gcgctcgccg ccggccggat
      781 gggaacctgg acgcgcgaat tggccgaacc cggctccagg ttctggtccc cggaactgga
      841 agccatcttt ggcctggccc caggccgcgc cccccaaacc gaccaggact tctacgaatt
      901 gatccacccg gaggaccggg gcgtggtgca aaagtttttc gcgcaggccg cggcccgcaa
      961 ggccgaccac gaagtcgagt tccgctttct gccccgccat cgccccccgg gctggatgct
     1021 ggcgcgcggg cgggtttatt acgatgcggc cggcaagccc ctccgcctgg cgggcgttgc
     1081 cattgacatc acggcccgca aggaagcgga gctggaaagc agccggctga acgaggaatt
     1141 ggagcagcgg gtgcgaaaac gcaccgcgca attcaaggcg gccaaccagg aactcgaagc
     1201 gttcacctac tccgtctccc acgacctgcg ggcccccttg cgcggaattc gcgggttcag
     1261 cgaaatcctg ctggcggatc acgcccacca actggacgcg gcgggacagg aactgttgcg
     1321 ccgcgtttgc gattccagcc agcgcatggg gtggttgatt gaagacctgc tggaactgtc
     1381 gcgcgtcggg cgcggtaaac tgcgctggca gccggtcaac ctctccgcgc aggccggggc
     1441 gattgccgcc agtttgcggc acgatgaccc ggcccggacg gtgcggttca ttatcgcgcc
     1501 caacctgcag gccgcgggcg atgagcgcct gctgcaggtg ctgctcgaca acctgctgcg
     1561 caacgcctgg aaattcaccc gctcccagcc ggacccccaa atcgagttcg gctttgtctc
     1621 ccacccggaa ccggcttttt ttgtgcgcga caacggcgtg ggcttcgaca tggcccacgc
     1681 cggcaagctg tttggggtct tccagcggct ccattccgac gcggaatttc ccggcaccgg
     1741 cgtcgggctg gccatcgtgc agcgcatcgt ccagcgccac ggcggccgcg tgtggggcgc
     1801 cggggagccc cgccagggcg ccaccttcta cttcaccctg cccgcgcagc ggcccctgga
     1861 actgtgagcc aggcgcttcg cgtattgctc gtcgaagatt cggccgacga tgccgccctg
     1921 ctccaatacg aactgcaacg ggccggctac gcgccctcct gccgccgggt ggaaacggct
     1981 gaaaccctga acgccgcgct ggaagagcag gcgtgggacc tcgtcatcgc cgaccaccgg
     2041 ctgccccgcc tcgacgccct ctccgcgctg gcccaggtca aagcccgcgg gttggacctg
     2101 ccgtttatca tcgtctctgg ctatatcacg gaagaggccg ccgtggccac catgaaggcc
     2161 ggcgcgcatg attatgtgat gaaggacaaa ctggcccgcc tgggcccggc ggtggcgcgc
     2221 gaattgcgcg aagccgtcgt ccggcgcgag cgccgccggg ccgaagacgc cttgcgccag
     2281 gcgtatgccg agctggaaca gcgcgtcgcg cagcgcaccg ccgagttaaa aaccgccaac
     2341 gcccgcctcg aaagcgccat cgccgaacgg cagcgcctcg aacacgaact gctggaaatc
     2401 accgaaaatg aacgccgccg catcggcctg gatttgcacg acgacctcgg ccagcagctt
     2461 tccggcctgg cgctcatgac caaggggctg gagcgcaaac tggcgcggcg caaagccggc
     2521 gaggccgaag acgccgcccg gatccaccag ctcgtgcagc agaccatcaa cctcacccgc
     2581 agcctggccc gcgacttcgc aacgttggac ctggaagcgg acgatctgcc cgcggcgctc
     2641 gatcgcctgg cggctcacgc cgcgtgggtg ttcaaaatct cctgccagct cgaacagtgc
     2701 ggccctctgc ccgcgctggg agcgggcatc gcccgccagt tatacaaaat cgcccaggaa
     2761 gccgtgacca atgccgtcaa acatggccag gccaaatccg tccgcatcgg cctggccagc
     2821 cgccccggcc aaatcatcct gaccctgcgc aacgatggcg cccccttccc caaactgacc
     2881 gagcctgcga ccggcctggg gctgcgaatc atgaattacc gcgccagcct cattggcgcg
     2941 tcgctggaaa tcaagaggga cagccccgcc ggcgcctgcg tgatctgcgc cgtgcccctg
     3001 cccgcggcgg aatcccgccc gcccccgccc ggcacccccg ccgcctgacc ggccccccga
     3061 cctcaatggc atcggccggg ttaatttttt gggttagttt tttgctgcaa acacttcccg
     3121 ttccggcgta tcatcgcgcg acttttcgtt atgggtgata ctcgtgcaag ccggcggctc
     3181 ggcaaggggt ccctttcagg gcgcctgccc ccccgcgtcg tcccgccatt tttcatgctc
     3241 accgctcatg tttgaacaag ccttccgaaa tattgacgac gtcctgcgca aggaggccgg
     3301 ctgcaccacc gagctggact acaccgaaca atcctcctgg ctcctgttcc tgaaatacct
     3361 cgacggcctg gaagaggaca aagccgccga ggccgccctc aacggcaggg aatacacccc
     3421 tatcctcgac aaaccctacc gctggaagac ctgggccgcg ccccgggacc gcgccggcca
     3481 gctcgaccac aacgccgccc tctccggcga cgacctccgc gatttcgtca acctcaagct
     3541 ctttccctac ctccacggct tcaagcagcg cgccaccagc ccggacacca tcgaatacaa
     3601 gatcggcgaa atcttcggcg agattaagaa caagatccag agcggctaca acctgcgcga
     3661 aatcattgac cacattgacg agctccgctt ccgctcccag aacgagaagc acgaactctc
     3721 gcacctctac gaagccaaga tcaagaacat gggcaacgcc gggcgcaacg gcggcgaata
     3781 ctacaccccg cgccccctca tccgcgccat catcgcggtc gtccagccca ggattggcga
     3841 gcgcatctac gacggcgcgt gcggctcggc gggcttcctg tgcgaatctt tcgtttacct
     3901 cacaacccgc ggcaagctca ccaccgcagg cctggccacc ctccagaccc gcacctttta
     3961 cggcaaggaa aaaaaagtcc ctcgcctacg tcatcgccat catgaatatg atcctgcatg
     4021 gcctcgaggc gccgaacgtc atccacacca acaccctcgc cgaaaacctc gccgaccttc
     4081 aggaacagga ccgctacgat gtcgtcctgg ccaacccgcc ctttggcggc aaggaacggc
     4141 ccgaggtgca gcaaaacttc cccatccgct ccggcgagac cgccttcctc ttcctccagc
     4201 acttcatcaa aatcctcaaa gccggcggcc gcgccggcat cgttatcaag aacaccttcc
     4261 tctccaatac cgacaacgcc tccgtcagcc tccgcaagct gctgctggaa agctgcaacc
     4321 tccacaccgt cctcgactgc cccgccggca ccttccaggg cgcgggcgtg aaaacggtcg
     4381 tcctcttctt cgaaaaaggc gcccccaccc gtaaaatctg gttctaccag ctcaaccccg
     4441 gccgcaacct cggcaagacc aacccgctca acgacgatga cctcgccgag ttcgtgaagc
     4501 tccaaaaaac cttcgccgac tcgccccaga gctggaccgt ggacgccaag accattgacc
     4561 aggccacttt cgacctctcc gtaaagaatc ccagcggcgg ggaggagata aagcaccgca
     4621 ccccccaggc catcatgaaa gagatcgccg cgctggacgc ggagagcgcc gcggtgctgg
     4681 gaaaaataaa ggccctgcta tgaagcgaga ggggaagaac gggaagaatg cgagtgatgg
     4741 gaacccccag cccccggact cccatccggc ccatgaggct gagccccttc tccccccgcg
     4801 cggcgattac cggacgctgc actctttcca caaagccgag gttgtctatg acatcacgtt
     4861 ccgctttgcg cacaaattcc tcgcccgggg cgaccgcacc attgaccaga tggttcagtc
     4921 ggcacgctca ggcaagaaga acatcctgga gggcagcaaa gcggcgctga cctccaagga
     4981 aaccgagatc aagctgacca acgtgggccg cgccagcctg gaggaattgc tggatgatta
     5041 caaagactac ctccgcgtgc gcgaacaccg cgaatgggac aaggactccc gggaagccct
     5101 ctacgttcgc cggctgggcc gcaaggtccc gcagacctac gagctttacc gcgcgttggt
     5161 ggagactcgc ccgcccgagg tggttgcgaa tatcgccatc tgcctgattc accaggccaa
     5221 ctacctcatt gaccagcaac tgcgccggct ggagaaggat tttatcgaac agggtggcct
     5281 gcgcgagcgc atgacccgcg cccggctcgc ttaccggaac aggaatcagc agggcagtgg
     5341 cgaaccgccg ccgcttcccc ggaaaactca atcgcccggc ccccatccgt cccctgactc
     5401 cccaaagccg ctgcccagga agcccggagg ccagccatga ctcaccccaa tccggcaacc
     5461 catcactccc atccgtccca tcactcccca gcccccgccc cgcgcggaag tgctggggaa
     5521 tatcaagagg ctgttatgaa aaaagggtgg cagataaaac gactcggtga agtctgcgaa
     5581 atcgtcaaag gccgaaagcc gatgttgaag ctgacaccgg cgagcggtga cttgccctac
     5641 ttggtcgcga aagtgatgcg cggcagcgca gaggcaaaat acgcctcagt caaagacaaa
     5701 aattcgataa cagttgaaga gtcggaaacc attatcattt gcgacggctc aaactctggc
     5761 gaagtcttca ccggctttcg aggcatcctt tcttcaacga tggggaaaat tgccaagaag
     5821 gtggagatag atgacaacta cctgcgcgcg tttctggcgt ccacgtttga agtctttaat
     5881 ggtacgaaga cgggcgccgc cattcctcat ctcgacaaag aggcgatgta tcaacttcag
     5941 tttcccctcc ccccgctccc cgaacaacgc cgcatcgtcg ccctcctcga ccaaaccttc
     6001 gccgccatcg ccaccgccaa agccaacgcc gaaaagaacc ttcaaaacgc ccgcgccctc
     6061 ttcgaaagcc acctccaatc cgtcttcact cggcggggtt cggggtgggt ggagaagacc
     6121 gtggacacgc tttccaccaa tctcgacagc aagcgcattc ccataaccaa gagtaatcgc
     6181 aaggccggat cgtatccgta ctatggggcg tcaggcatcg tggactatgt cgcagactat
     6241 attttcgaag gcgatgcgct cctcgtttcc gaagacgggg cgaacctgct catgcgctcg
     6301 acgccgattg cattttctgt gtccggcaaa tactgggtca acaaccacgc gcacattttg
     6361 aagtttgaga acatggcgac tcagcggttc gtcgagttct acctggagag cttaaagttg
     6421 gacgagtaca tccgaggcgc ggcgcagcca aaattgaccc aaaaggccct taactcgatc
     6481 ccaatcccga ttcccacaac tttcgaggag caagcgcgga ttgtcgaaaa cctcgactcc
     6541 ctctacaaag aaacccagcg cttctcatca atctacgagc gcaaactcgc cgccctggat
     6601 gagctgaaaa aatccctcct ccaccaggcc ttcaccggag aactctgatg cccgcactct
     6661 ttccaatccc aacgggattg catccttcag cccagggttg cgaggcacga gctaccctgg
     6721 gtcacgcgtc ccaacatttt ccccaacccc aacggggttg catcaccgct ccaccgcctg
     6781 cccgcggcaa ccccgttggg gtagtaagaa agccctggag gagggcaagg accagccatg
     6841 aatgaagctg aaaccagagc cgagtacgtt gaccccgcgc taaaagcggc cggctggggc
     6901 gtcgtcgaag gcagccgcct ccggcgcgag tatcccatcg ctcccggccg cctcgagggc
     6961 cacggcaaac gcggcaaggc gctcaccgcc gattacgtgc tggaatatcg caataccaag
     7021 ctcggcgtcg tcgaagccaa ggcgctggac gaggagttga ccgaaggcgt agctcaggcg
     7081 aaaaactatg cgggcaagct ggccgtccgc catacctacg ccaccaacgg gcgcggcatc
     7141 tacgccattg atatgcagac cggcgcggag ggcgaaatcg ccgcctaccc gacgccggac
     7201 cagctctggg ccatgacctt cccttcgaca cgctcaggga ccggagatgc cgccgacagg
     7261 gccagtgagc ttgccgaact gtggcgcgac cgctttgccg cgattccctt cgaggacaaa
     7321 gggggctcgc atcccagccg ctattaccag gacatcgcca ttgagcgggc gatgcaggcc
     7381 atcgcccggg gccagtcccg catcctgctc acgctggcca ccggcaccgg caaaaccttc
     7441 atcgccttcc agatcgcctg gaaactcttc cacgcccgct ggaacctggg cggccagccc
     7501 acccgccgcc cgcgcatcct attcctggcg gaccgcaacc tcctcgccaa ccaggcttac
     7561 aacgcgttct cggcgttccc ggaagacgcc atggtgcgca tcaaacccga ggacatccgc
     7621 aagaagggca aggttcccaa aaacggcagc ctgttcttta ccattttcca gacgttcatg
     7681 gcttcggcag gctcagccac cgagaaaggc gcgatccctg agcctgtcga aggggcggcc
     7741 tcggaactgc cggaagttgc tgaaccaacc gagaaggcga gtggctacat gtatattctc
     7801 gggtgtggcg atggcagctt ctacacgggc agcaccacca atttgcatct gcgcctggag
     7861 caacacagaa gcggagaagg cgcgcaacat acgaaggcgc ggctgccgat caaactcctc
     7921 tattacgagg catttgatcg tatagatgcg gcatttgccc gcgagaagca ggtgcagggg
     7981 tggagcaggg ccaagaagtt tgcgttgatc caacgcaata tcggtctctt gaaacgcctt
     8041 tcgagcgcct ctgccgacgg tcgcccggtc cctgagcctg tcgaagggtg cttcggcgag
     8101 tatccgcccg acttcttcga cttcatcgtc attgacgagt gccatcgcgg tggcgccaac
     8161 gacgaaagca actggcgcgg gatcatggaa tacttcgcgc cggccgtgca gctcggcctc
     8221 accgccaccc ccaagcggga ggagaacgcc gacacctacc gctatttcgg cgagccggtg
     8281 ttcatctact cgctcaagga aggcatcaac gacggcttcc tcacaccctt ccgggtgaag
     8341 cagatacaaa cgacgctgga ttattacgtc tatacgccgg atgaccaggt ggtggagggc
     8401 gagattgagg cgggaaaact ttacgacgaa ccggacttca accggatcat cgagatcaag
     8461 gagcgggagc agaagcgcgt cgagatcttc ctgggcgaga tcaaccagaa cgcgaagacg
     8521 ctggtgttct gcgccaacca ggctcacgcc ctcgtcgtgc gcgacctcgt caaccagctc
     8581 aagaccagca aagacccgaa ctattgtcag cgcgtcaccg ccaacgacgg tgagctgggc
     8641 gagcagtact tgcgggattt ccaggacaac gaaaagtcca ttcccaccat tctcaccacg
     8701 tcgcaaaagc tctccaccgg cgtggatgcc cgcaacatcc gcaacatcgt gctcatgcgc
     8761 cccatcaact cgatgatcga gttcaagcag atcatcggcc gtggcacccg gctctttgac
     8821 ggcaaggact acttcacgat ctacgacttc gtgaaggcgc acctccactt caatgatccg
     8881 gagtgggacg gcgagcccgt tgagccggaa tcatgcccgc aatgcggctg ctctccctgc
     8941 gtctgcgaaa cgccgccgcc ccaaccgtgc ccggtctgcg ggaaatcgcc ctgcgcgtgt
     9001 ccgaaagcgc cctgcccggt ctgcggccag ttggtctgcg tttgcaacca gaagtcaaaa
     9061 gtgaaagtga agctcgccga cggcaaggag cgcaacctcc ggcatatgat ggcgaccacc
     9121 ttctggcatc cggacggcac gcccatgtcc gcccggcagt tcatggagcg gctcttcggc
     9181 aagctgcccg atttcttcca aagcgaagcg gagctgcgca atctctggag cgcgccggat
     9241 acgcgcgcga agctgttgca ggggcttgcg gaaaaaggtt tcggccgcgc ccaactggcc
     9301 gagatgcaga agatcataga cgcggagaag agcgacctgt tcgatgtcct ggcgtacgtc
     9361 gcctatgcgt tgcccccgct gacgcgggcg gcccgggccg ccggggcgcg ggtgtatatc
     9421 aattcccact tcaacgccaa gcagcgggcc ttcctcgact ttgtcctgtc ccattacgtc
     9481 catacgggca ttgaggagtt gagccgggag aaactgcggc ccctgctgct gatccgctac
     9541 cacaactcga ttcaagacgc agtggccgac ctcggccagc ccgcgcaaat cggcaacctc
     9601 tttgccagct tccaaaagta cctgtaccag cccggcccca ccccggcctg acacgatctt
     9661 ttgttctcgc cagaccccgg atgctggcgt cgtccccggc ccaaattgaa gcgcgccccc
     9721 gctcacctcg ccgccgctgg caccgtcctg gccacgatct ggctcggatg ctcccgcgca
     9781 taaatcggat ttcttcccat ccaatcccgt gtcatcacct tgtcgtcacg gcaagcggct
     9841 ctttgaaacc tccgaaaaaa gtggtgcccg cgacaggact tgaacctgta cgatgttact
     9901 cactagaacc tgaatctagc gcgtctgcca attccgccac gcgggcatga ttgacgcctg
     9961 cccccggcgt tggggcgctc atttcccgcg ccttagtccg gttgcaatac gttcacaacg
    10021 gcatttacta tgcccgaatt ggagcaaaca gcaagcggtt tccgcatttt cctgaatccc
    10081 ggttccgggg gccgcgtttt ccattgagtc attttgcggg gctgattagc ctaggcgttc
    10141 atgaacattt ttgtcacggg cggggcgggc tatatcggtt cgatttgcgc cgaagcgctg
    10201 ttggaggcgg ggtatgccgt gacggtgtat gacgatttga gcgagggcca tcgttcggcg
    10261 gtggaccggc gggcgcggtt cgtgcagggg cgcccggacg aagccggcca cattttcaac
    10321 gccatccagg aggctcggcc ggacgcgatc atgcactttg ccgccaatgc gctggtgggc
    10381 gaatccatga ccaacccgag gaagtatttc cataacaacg tcgtcaatgg gctcaagctg
    10441 ctcgatgccg ccatcgaggc gggggtgaag aagtttgtat tcagttccac ttgcgccact
    10501 tacggcccgc ccgagcgcct gccgatgacc gaggatctgc cgcagcgccc catcaacccg
    10561 tatggggaat ccaagttgat gttcgagaaa gtgctttcgt ggtaccagcg catctacggg
    10621 ttggagttca tcgcttttcg gtatttcaac gccgccgggg ccagtgaacg tttcggggag
    10681 caccatcgga tcgagacgca tctgatcccc aatgtcttga aagtcgccct gggccaaaaa
    10741 ccacattgtg agatttacgg cacggactat cccacccccg acggcacctg tattcgggat
    10801 tatattcatg tgcgggattt ggcccgggcg catatcctgg ggctggcgcc cggccgccag
    10861 ggattctaca acctcggcaa tggcgatggc tattccgttc gccaggtgct tcaaacctgc
    10921 gaaaaggtca ccggcaaaac cattcccgcg ctggaaaaac cgcgtcgtcc cggcgatccg
    10981 cctcgcttga ttgcttcggc ggcgcgggcg atggaggaac ttggctggca acctgagcat
    11041 ccccggcttg aggacatcgt cgccagcgcc tgggcctggc atcagcggca tccccagggt
    11101 tatccggact aactttatgt taacgcccgc cggttaaccg gcgtaaccgg cggttgtgcc
    11161 gcgccattcc gccccgtttt gcggccaaaa tcatcctgcc tttggtccgt gtttggtctt
    11221 gtccgcgcgg gttcgctgcc gcggcgcagg cggagtgggg cgatggaaag cggggggcgg
    11281 gagtttggag cggcggcggg agggttggag agggactggc atggcagggg ttgtttttcc
    11341 cggtttccaa ccgcccggcg gcacgttata aatcttgcgc atggctcaat atcgcgtcgg
    11401 aattattggc ggcagcggac tgtatcatat tgaaggcttt acccgtcaga aatgggttaa
    11461 ggtcaagacc ccgtttggtt cgccttcgga tgattttctc acgggcaaac tggcggggcg
    11521 ggaggtggtt tttttacccc ggcacgggcg gggccaccgg attttgccca gcgagctgaa
    11581 tcatcgggcc aacttctggg ctttgaagaa gctgggggtg gcctgggtga ttagcgtgag
    11641 cgcggtgggg tcgctgcagg cccggtatcg gccgtgcgac attgtattgc cggaccagtt
    11701 ttacgaccgc accaagcaat ccgccgcgca cagctttttt ggccgcggca tagtggggca
    11761 tattgcgttt gcgcagccca tttgcgagga attgcggcag ttgctgctgc aggcggcgcg
    11821 ggcgctcaag gcgcgcgttc atgacggcgg cacctacgtc aatatggaag ggccggcttt
    11881 cagcacgcgc gccgagtcca tcaccaatcg caagctcggc cacgatgtca tcggcatgac
    11941 gaacctcggg gaagccaaat gcgcgcgcga agccgagatc gcttacgcca cgatggccat
    12001 gattaccgat tacgattgct ggaaagagga ggaggcgccc gtgacggtgg aaatggtgat
    12061 cgccaatctc cggaagaacg cccaaaccgc ccaggcgatc attgcccggg tgctgccccg
    12121 gatccccgcc gcgccgaatt ggccctgcca cgacgcgctc aagaatgcca ttatgacgga
    12181 ccggaaatgc tggcccgccc ggaccctgcg cgagttggag ccgatcctgc gcaagtatca
    12241 gtgagaggga cgggagctgc ggcgcggctt gaccggcccc ggccaggcgc taagattccc
    12301 gcgcgatgac ccaaaagacc atcattaaac ccgcccggtc ggcgcccgcg gcggggccgt
    12361 acaaccacgc ggtgcgcgtc ggtgatttgc tgttctgcgc cggccaaatc ccgattgatc
    12421 ccgccaccgg cgagctcgtc ggcggcggca ttcaggcgca gacggaacgg gtgttgcaga
    12481 atatcaaggc catcctggac gatcagaagc tgacgtttgc ccaggttgtc aagagcacgg
    12541 ttttcctgac gaacctggcc gacttcgccg ggatgaacga agtgtatgcc cggtttttca
    12601 cggcggattt tccggcgcgt tcgaccatcc aggtggccgc gctgcccaaa ggcgccagcg
    12661 tggaaatcga ggtcgtcgcc cattactgat gcggcgattg gccggcggcg gccccggatg
    12721 ccccatgttt gccaaacctg acccgcggct gtgattcatc ccaccgcgat aattcatccc
    12781 cgcgcgaagc tggacccgac cgtgcaggtg ggggcgtatg cgattataga cgcggacgtg
    12841 gaggtgggcc cgcactgcgt gattgggcct tatgtttacc tgacgggcgt gacgattatc
    12901 ggcgcgcata atcggttcta tgccgggtgc gtgattggcg aggcgccgca ggatttgaaa
    12961 taccacggcg agctgaccgg cttgcggata ggcgaccata atgtcttccg ggagcacgtg
    13021 acggtgcatc gctccaacca ggcggcggag gagacggtga ttggcgcgcg gggattttat
    13081 atggcccact cgcatatcgc gcataactgc cgcctggggg atggcgtcat ccttgccaat
    13141 ggcgtgctgc tgggcgggca tgtgacggtg gaggacggag cgtttctttc gggcaattgc
    13201 ctggtccacc agtttgtgcg cgtgggcacc ctggccctga tgcagggcgg ctccgccatc
    13261 agcaaagacc tgccgccgta caccgtggcg cgcgggggca accgcctggg cggcctgaac
    13321 atcatcggcc tgcggcgggc gggctttagc ccggcggaac ggctggaact gaagcagctt
    13381 taccatgcgc tgttccggaa agggcacaat ctgcgggcgg cggcggcggc ggcgcgggag
    13441 aaattcacga gcgcgcccgc ccggcgcctg ctggattttc tggcggcggc ccggcgcggt
    13501 gtctgcggcg aggcggacga gcaggaggat aaaaacattg acgaagcccc cgctggcggt
    13561 taggctggtt ttatccatgt gcgtcaggaa ttggtgtatg aaatggttgg gaggagcggc
    13621 cgcgctgctg ctggccgccg ggttgggcgg ccaggcgcag gccgccggcc ggtcagccgg
    13681 ccggcccatt caatttaccc ccgcgcgaag cgacgaggtg ttgaccaacc tgcaccagct
    13741 ctcgaataaa aaggacagcc tgaagcggct ggaggaagac ctcaacgcgc cgctgcaaag
    13801 cctcgcgccc cggggggccc tcgaaggcgt cccggcgaga gtgtcgcgtc cttccgcccc
    13861 gtcagccgtc cagagcaagc gagcccggga actgctggaa cgccgcaaga actggatctt
    13921 catggcgccg gaagacatca tcggcaaccc gaccgtggaa caagtcctcg aagcgcccga
    13981 gttcgacgag aacggccaaa tcaagaaaga tttgcccgcc atcgaaagct attacctgcg
    14041 cctgctgccc aagcggccgg cccggcccct cccggaatcg tcgcccgtgg aggatgtgtt
    14101 cggccgcgca aacaagccga acatgcgcga agcccccttc agcgatgatg cggacctccc
    14161 cgccgggatc cgggaaagcg ccgacgcgct caggagtttt gttcagcccc gggagggcga
    14221 cgcatcttat tttttaagca cgccggctgc ggatttttcc ctggcgcacc ggctccacag
    14281 caaggcggca tccgcggagc agatgctgga gcataataaa cgcatggacg aataccgcac
    14341 cctggtggat cccaactggc gttcgtcctc cgcgacgacg ccggggcttc cggcgctcag
    14401 ttctgtcggg atgccgccgc cttccgcaac cccggtgaac aagcagcccg ctgattccga
    14461 tttggcgtcc gcgcacggat tggaagcgca gatgaacatc cgcaacccgc tgcttggtcc
    14521 gcccggattg cccgacgtga acgccaaggc gctcggccaa tcccggcccc tgccggtgga
    14581 gaccaaggtc cgtcagacca aaatggcaac gacggagccg atgtataccg cgccccggcg
    14641 ggcgttttag ccgaagtttc cggcgctgaa accggttagc cgcgcccgcg cgcccccgga
    14701 agccccggtg gcgatggtac cgcgggcagc cccgaattat atcgttgaca ggcagggttg
    14761 gcgtctgcgt attgtctggc catgcttgcc gtcggtttgg agtcggatcg gataatcaag
    14821 tctgccgctt aactatgaat caacaacctg catcatccgt tggcccgctt ccgggctaca
    14881 tcaccagcgc cgttcccaat ccccgggcca accgcgcccc ctggtataaa aacaccgccc
    14941 cgacttacgc gggcattttt ctgtggtttg tcttttggca ggatgccgcc aaaggcggcg
    15001 cgcccggggg cacgctggcg cagggggtgg gctgggcgtt gttgagcctg gtcattgcgg
    15061 cgttggtctg ccattttctg ttttacctcg tgccgggcct gttcggcatg aagaccggcc
    15121 tgccgctgta cattgtcggc acttcggcct ttggcgcgca gggcggcttc ctgatgcctg
    15181 gttttctcat gggcgtgctc cagtttggct gggtgggggt gaacatctat ttttcttcac
    15241 tggcgctgag cgcggtgatt cccgttgacg cgcgtttcat catggtggtg tggggagtgc
    15301 tggcgtgttt tgtcgggctt aaaggcattc aatacgttgc cagggtggcc acctacctgc
    15361 cgctgatccc gctggtggcg ctgctctggc tggcggccgc gaccatcggc catgtcggca
    15421 gcttcaagcc cgacgcgctg atcgcggcgc aaacggcggc caacccggcg gggccgggcg
    15481 cgctgggcct gctgggcgtg ctggccgcca tgctgaccta cgtggtgggc ttctttgcca
    15541 cggcgggcgc cgcgggggtg gattttggtc tgaacagccg cgatgaaaag gatgtgagcc
    15601 ggggcggctt gttcggcatt gcgctggcca ttgtcgtcac cggcggcctt tcgctgttgg
    15661 tggtggcggg cacttacggg agcatgaaag agacgattga cgccgtggcc acccggcaac
    15721 tcagcttgag ttcttttgac ctgatccccg cagtgctggg cgcggccaca gccaagtggg
    15781 tgatgttttt gctggcggtg gcggcctttc cgcccgcctg cttctcctcg tttatcgccg
    15841 ccaacagctt caaaaccacg ctgcccaaag taaatccctt tatctcggtc ggcatcggga
    15901 cgggggtggc gattgttttg gccatcaccg gcgtggtggc caaggccatt gcggtgtttg
    15961 tgatcatcgg cgcttcgttc gggccgatct gcggcgcgct gctggtggac tacctgttga
    16021 gtggccggaa gtggagcggg ccgcgcgcgg gtttcaaccc ggccgggtgg ctggcctggg
    16081 cgctcggctt tctggtgggc atcctgccca acctcggcgt ggggcttccg gcggcgcccg
    16141 tgctggcgtt tgccgttggc gcgggggtgt atttcctttg cgccaaactg gggctgctca
    16201 cgcccgtagt gccggtctcg ggcgcgctgg ccgaggcgac ccggtaggcc gcaggctgga
    16261 gcggccgccc tccgcggtga accgcgcgcg cggaggcagg cgccggattc tgccggcgcg
    16321 ggtttttacc cttcggcgcc gttgccgttg gcgcgccgtt cccagcggcg cggggagcgt
    16381 tccggcaggc tggtgtccac cttggcgtag taggtctccg gtccaaagcg ttcgttgacg
    16441 gcctgctgca attggcggga gggcgccacc gcgaactggt catgggcttc gatgaagatc
    16501 attcccccgg ccggccgcat caggcataga aagagcgggc acttgccccg gtgggcgcgg
    16561 gccaattcgt ggatttgctc caggtgccca ggctgcaggt gcgcggtatg gagccgcaga
    16621 tgcacctgtt tggtgaaccg ccccggcgcc tcctccagcg gcatgatttc ctgcgggaac
    16681 agcttgggtc ggtcatcccc gtggccgacc tctccaatga ccaggatggc cttgcccggc
    16741 acgagcagtt cccggtattt ctcgcaattc tcatggaggc aaaccacctg caccgagcct
    16801 tcgaggtctt ccagggtggc caggacgtag ggcttttgag tctttttgga agtgccgctt
    16861 tgcacggcgg cgatgaggcc gcccatgcgc gtgaggctgc gcgccggcag ttgggcgatc
    16921 gaggcggtgt tggccagggc gtatttttgc aggatcggca cgtaaggggt cagcggatgg
    16981 ccggtgacgt agaagcccag caactccttc tcgcaggcca gcaactggtg ctcgggccat
    17041 tccggcaggc tggccgccgg ttccgccggc accgcatgct cctccagggt gtcaaacagg
    17101 gaagcctgcc cgcgctgccg gtcggcgatg acgctggcgg cgcgcgccag ggtgtggtcg
    17161 agcaggccgc agagcatgga gcgcgtctgg ccgaggctgt cgcaggcgcc gcacttgatc
    17221 aatgcctcca ggattttgcg gttcaccgtg cgggcatcca cccgttcgca taaatcggcc
    17281 agcgagcgga atcgcccgcc ttcgtcccgg gccttgagga tggcctggac cgcgatctcg
    17341 cccacgccct tgatggcggc caggccgaag cgaatggccg cggcggaggc agggtcgcgg
    17401 tcagcgtccg ggccgggcgg gcgggccgcc ggcgttcccg gggcgggagc gaaatagacc
    17461 tggctctcgt tgacgtccgg tgccaggacc tcgatgccca tgccgcgggc ctcggcgatg
    17521 tactggctga gtttctcggt atcagccagg tcattggtca ttatggcgca gaagaactcg
    17581 accgggtaat tggccttgag ataggcggtc tgataagcca ggacggcata ggcggcggcg
    17641 tgcgatttgt tgaagccata gccggcgaac ttcgccagca agtcaaaaat ctggccggct
    17701 ttggcggcgg ggatgcggtt cacccgcgca cagcctttta cgaagggctc ccgcaggctg
    17761 gccatttcat cggcctgctt cttgcctatc gcccgccgca gcacatccgc cccgcccagc
    17821 gagtagccgg ccagggattg cgcggcctgc atcacctgct cctggtaaat catgatgccg
    17881 taggtctcgc cgcaaatcgg ggccagcagc gggtgcacat actcaatctg gatttcccca
    17941 tgccggcgct tgatgaattc aggaatcagc tccatgggcc cgggccggta gagggcaatc
    18001 agcgccgtga tgtgctcgag ggaatcaatc tggaacttgc ggcacagatc gcgcatgccg
    18061 ccggattcca actggaaaac cccgagcgta ttggctttgt tgagcaggtc gtaggtcttc
    18121 ggatcatcga gcggcaggtc gtcaatgggc acttcgatgc cccgggtctg ccgcactcgt
    18181 tcgcaggtgt tgcgaatgac cgtgagggtt ttgaggccga gaaaatccat cttcaacagg
    18241 cccagtttgc ccaccggccc catggcgtac tgggtgacaa tagcgccgtt ctcgtcctgt
    18301 ttgagcggca gcaggttgac cagcggctgg tcgccaatga cgattcccgc cgcatgcacg
    18361 gaagcgttgc gcgtgaggtc ttcgagcacc cgcgccgtgt caatcaactc gcgggtgact
    18421 tcttcgttct cgcaggcctg tttgagttcc ggcgaccgct tgagggcttc ttccagcgtg
    18481 atcttcagct cggaagggat catcttcgcc agccggtcgc cctcgccaaa ggtcagcccc
    18541 atcacccggg ccacgtcgcg caccaccgat ttcgcgccca gggtgccgaa ggtaatgatc
    18601 tgcgcgacgg cgtcctgccc gtacttctgg cggacatatt cgatgacttc ggcgcggcgg
    18661 tcatcggcga aatcaatatc aatgtccggg ggattgacgc gctcgggatt caggaagcgc
    18721 tcgaacagca gcccgtagcg gatggggcag acgttggaga tgccgagcag gtacgtaacc
    18781 agcgaacccg ccgccgaacc gcgcgcgctg cacgggatcc cgtgcgcgcg cccgtagcgg
    18841 atgaaatcct ccacgatcag gaagtagctc acgaagccgg tctcctcgat gatctggagt
    18901 tcgcgctgga gacgctccag gagcgcccgg agcgcggggt cgccagcggg cgcggcggcg
    18961 gccggggaac cgcccggaga cgccgccggg gccggggcgc cgggcagccg cgcggggtct
    19021 tcgacgtgtt ccacgacaaa ctcgcggccc acggcccgcg cgtggatgcc gtaacgcgcg
    19081 cgcagccctt cggccgccag gtgccgcaaa tagccctcgc gcgtaaaatg ctccggcggg
    19141 gtgaacaccg ggtaatgcat ctgccggaaa ttgaattcca ggttgcactt ttcggccacc
    19201 tcgagcgtat tccgcacggc ctccggcacc tcggcaaagc gcgcggccat ctccgccgcc
    19261 gagcgcaggt agtactgccc gtcggcgtat ttcaggcgcc gggcatcgga caacagcgtc
    19321 tgcgtgccga tgcaaatcag gcaatcatgc gcatgcgcgt ggtcccgctc gaggtaatgc
    19381 acgtcattgg tcgcgacgag cttcaggccg aactctttgg accacgccac cagctgccgg
    19441 ttgaccctgg cctgctcggg caggccgtgc tgctgcagct ccaggtagaa attctccgcc
    19501 ccgagcgtct gcttgaacca gtccaccgcc gcgcgcgccc gcggcaactg atctttcaaa
    19561 atccactccg ggatttcgcc cgccaggcag ccggagagcg ccagcagccc ttcccggtgt
    19621 tccgccagca gttccttgtc aatgcgcggc ttgtaataat acccctccgt atgcgcccgg
    19681 gtggccagtt ggatcaggtt ccgatagccg gtttcatccc gggccagcaa cacgaggtga
    19741 tgatacacat cgcgcccgcc ggtggtggtc ttcttctcga accggcttcc cggcgccaca
    19801 taaacctcgc agccaatgat gggtttgagg ccgtgcttcc gggcctgctc atagaagtct
    19861 atggcgccat acagcacgcc atggtcggtg atcgccagcg ccggaaactc caggtcgcac
    19921 gccttcttca tcaaccgatc cagccggcag gcgccatcga gcagggaata ctcggtgtgc
    19981 aggtgcaggt ggacaaattc tgcgtgcggc atgagagcag tttagggcgg agccgccacg
    20041 ccgggcaagc ccgggatgcc ccggcgcgcg cgcggcttcg cgcgccgggc cggcgttaaa
    20101 aagcgcggcc cgcccgggcc ggccggcggc tggctatttg gtggccggcc tggcgggagg
    20161 cgcctccggc gaagcgggcg gttccatggc cttcaacttc tcgtccagaa tctcgacgcc
    20221 ggcctcggcg cggagtttgg cgacgtagtc cggcagttgc ttcagcagcg cctgctgcgc
    20281 caggttgtcc ttgatctcct cggccacctt ggccagctcg attttgtggg ccgggatctt
    20341 ttccagcagc ttgatgatgt gatagccgta gcgtgtggtg acgatgtcgc tcacctggtt
    20401 ggtcttgagc ccgaaggccg cggtttcaaa ttccggcacc atctggccgc gggggaaggt
    20461 gtattcgccg cccttgtcct tggagccggg atcctcggag aactcccggg ccagcttggc
    20521 aaagtcttcc ccgtcccgcg cgcgcttgag caggccttcc atttgcttgc gtttggcggc
    20581 tttttgctcg tccgtcatct ccacgccggt cttggggtcg agggtggtca gcaggatatg
    20641 gctggcgcga accatttccg gctgttcaaa atgcgccggg ttgtcctcat aaaaggtctt
    20701 ggcgtcagca tcggtgatgt tgaccttgag ctcgcgtttg agcacgtttt ccgccgtcaa
    20761 gctgtccgtc caccgggcga gcacctcctc gccggtggag ttcatgagct ttaattgccg
    20821 gttaaacgcc tcctccgagc cgacgcgggc tttggcttcc tcgaaccgct tttccgccag
    20881 cgccttgccc gcggccttgt cggcggccgt ggctttggcc ttgatgagtt gtatctggat
    20941 aagctgatcg agcacctgcc gatccagcgt ggccagttgc gcgggcggca gcgtctggcc
    21001 gcgcccggcc agttgggcct tgaggcggat gatctcgtcg tccagcaggg cgcgggtgac
    21061 ctcgacgccc ttgccccggg ccaccacggt gttggtgaag agcgcgctgg ctttgccatc
    21121 ggccgcggcg gggggcgcgg cggcgggcgg cggcggggcc ggctcggcgc tcccggcggg
    21181 gatggcggcg gccagcagca gcgggagaga gaatcctaat atccgggttt tatccatgaa
    21241 cttgctttcg ttgatgacta cagttttact acctttacat tgctgatatc ggggtcgttt
    21301 tgaatctcgt ccaatacctc ctgaggcggc acactatcca aattgaggac cgtcaaggcc
    21361 cggccgccga cggtgtcgcg gcccaggttc atgctggcaa tattcacctt gtgccgggcc
    21421 agcagggtgc ccaggtagcc aaccatgccc gggcggtctt tgttgctcag caagagcacc
    21481 acgccctcgg gcacgatctc ggtcggctgg ctgaacacgc gcacaatgcg cgggttatcc
    21541 ggcgagccaa agaacgtgcc gccggcggaa atcaccttct ggccattgcg aaacacctgg
    21601 acatgcagcc attcgttgaa cgtcaccggc tcgttcgagc gcttctcctc gaccgtgatg
    21661 ccgagcgtcg cggcgatttt gcggacattg acgttattca gctccttcac cgcggcgcag
    21721 gacaggaatc ccagcaggac ggcgcgcgtc accgggtcgg tattgggcag ctcttgcgcc
    21781 ttgccgccga aggtgaggta cagccggtcc acctgcgccg gggccagttg ccccagcaag
    21841 cgccccagtt tctcgcccag gaccaggtac ggcttgacct gctcatacgt cttcgcgtcc
    21901 aggtagggca ggttgacggc gttgcgcact tcgcccgtca acaggtaggc ggcgatgacc
    21961 tcggccactt ccaggccgca tttttcctgc gcttcgaccg tgctggcccc caggtgcggc
    22021 gtgagggtga tcgccgggtg ccgccggaaa ggatgatcgg cgggcagcgg ttcggccgcg
    22081 taaacatcca gcgccgccgc cgccaccttg cccgagtcca gcgccgccag caggtcgttc
    22141 tcgaccagga cttcgccccg ggcgcaattc accaggcgca ccttgggctt cattttcgca
    22201 aacgccacgg cgttgagcat gccgcgcgtt tgttcggtga ccggcatgtg gacggtgatg
    22261 aagtccgcgt cccggtacac attgtccagg tctttgacca gctcgatgcc cagcgcctgg
    22321 gcgcgcgctt ccgtcacgaa cggatcatac cccagcacgc gcatcccaaa ggccacggcg
    22381 cgccgggcca cctcgctgcc aatgcgcccc aggcccagca cacccagcgt cttgccggtc
    22441 agctcgatgc cttgaaattg cttccggtcc cactggccgg cgctcatcga ggcgctggcc
    22501 tgcggcacct tgcgggccag gttcagaatc atcgcaaacg acagctcggc ggtggagatc
    22561 gtgttcccgc ccggcgtatt catcaccacg accccatgct ccgtggccgc cgccacatcc
    22621 acgttgtcca cccccacccc ggcccggccc accacccgca actgcggcgc cgcctcgatg
    22681 actttgcgcg tcaccttcgt ctcggagcgg acgaccatgg ccatgacgtc tttgaccatc
    22741 ggcagcagct ccgcttcggg aagccgcttg ggcagcacga ccacctcgaa ctcggggcgc
    22801 tgttgcaata cggcaatccc tttgggcgat acaggatcac ataccagaat cttcatagga
    22861 aagcgccact ataggcgggg ccccgccgaa ggtcaaatgc tcaatccgca ccctttcccc
    22921 cgcggacagg gtctttcgtt atcggcgcct gcgtttcgat aactgcggtg gaaacggctt
    22981 tcccaacccc aaaaccgctt ttcgggttga gggagcgagg agaaaagcat ctattgcttc
    23041 aaagctgcag gcccgccctg ggggcaacga aagcagtcgc ctccgcccgg gtggggaaac
    23101 gtccccgcca gccgtggctc catcccaccg aaaaatcagg gtgcgcgaca acacctcacc
    23161 gcccgtctcg gctttgccga acgttcgcgt cctctccctc ccgccttgga acgtcctttt
    23221 acatcatccg cgactcgtcc attcctcctt tccggctcag gcccgcgccc a
//
LOCUS       DAOPQG010000058        22865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2805_length_22865_cov_17.759448, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000058 DAOPQG010000000
VERSION     DAOPQG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 22865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22865
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_22865_cov_17.759448"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1267..2604
                     /locus_tag="PKI07_08155"
     CDS             1267..2604
                     /locus_tag="PKI07_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HOC51063.1"
                     /translation="MLPSAIMGATSPAVRQRGKVAKPPRGGARPAPSRRVRTQAGPGP
                     AAAVRQAQVAQGQATHLALMVSPTERGTFRSQARAVWSALRAALEQPPGTKAITALTV
                     FLKDPRQQRECERVCADGLGPERPFINYVWQPPCDGAALALEAWAIGGPAVRCERRGP
                     HALVVDCDGVRWVYCAGIQAAARGVYRQTLAALAQSRAALARAGSRFERVVRTWFYLG
                     GITEPEAETWRYQELNRARTDFYRAVRFHGAAPDPGAAAGIYPASTGIGMRGRKLALS
                     CLALETRRSDVHLRALENPRQIPAYAYDARYSPQSPKFSRAVALRVGDHVTTWISGTA
                     SIVNSETRHPDGVEHQTEQTIDNIERLIAADNFARHGLAGAGARLRDLAGIRVYLKRP
                     ADWKRCRAICERRFGPVPALYVVADICRPELRVEIEGVAFSRLGAPPAPREVA"
     gene            2668..3834
                     /locus_tag="PKI07_08160"
     CDS             2668..3834
                     /locus_tag="PKI07_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC51064.1"
                     /translation="MSKSETMTPRERVLKALNHQIPDRVPIDLGGNQTGIHRNAYRSL
                     LQHLGVTDEIRIMDAVQQLAAPGEQVLQRFHVDTRYVAAGAAASWKGGIVPARRDGRN
                     WDDLTDEFGVRWSMPEDAPHYMDITLHPLANATLAELRDYPWPRGDDPSRFAGLRERA
                     LTLKRETPYAVVSGISGVVYEICWYLRGLEQWFCDLMYQPEFCAAMLDQTLKFWLDWF
                     RLFLDEVGDVVDVIMIGDDLAGQNGPLFNPAIYRQIVKPRHQRLVQYLRSRTAAKIWY
                     HTCGACQDFIPDLIENGVQVLNPVQVSARNMDPAELKRRFGRQIAFWGGGVDAQHVLS
                     RGTPQEVAANVRQNLQALMPGGGYIFNNVHNIQGEVPPENVVALFDTAYEHGFY"
     gene            4024..4950
                     /locus_tag="PKI07_08165"
     CDS             4024..4950
                     /locus_tag="PKI07_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51065.1"
                     /translation="MTGKAALVRMARFATLALALGTPVLALSGAEVTLTQTNWVERWI
                     TNVIEVRMPLNQFVNEYRTNWVTQFQTNVVNVFATNRVTRTLTNTVVVDLVQTNFVVG
                     YQTNRVVRMQTNYAAVNLFQTNFVDQCQTNWSVLTLTNWETVVLFRTNWVVQPATNVV
                     QIELPPSPAPAVAAAREAAAAPEPPAAAPAKAPAPVPPPAAWDGPLALEVVRTARPAA
                     QHPIEVLLKVRWTDSTAAPPPVQQWRIEREDAAILLFGQEQEFKRQLPPGKYKVEARL
                     KAAGDDLPFSVRGILAVTTTDAVIQPRLLVQK"
     gene            5180..5437
                     /locus_tag="PKI07_08170"
     CDS             5180..5437
                     /locus_tag="PKI07_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51066.1"
                     /translation="MKTFLRDVATRQYFRSADHWTFDREEAHDFGFIPQAMRIARKLG
                     VSTLEVVVSFEDSLPLANMSLADFLRGLSRPGRRRTAGSRA"
     gene            complement(5495..6793)
                     /locus_tag="PKI07_08175"
     CDS             complement(5495..6793)
                     /locus_tag="PKI07_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51067.1"
                     /translation="MRDQPAQTPPANTAAHRASFFRQSGWLMFATIAGGLFMTAMHFL
                     SKARALPEAEYAQFGVFLAVTMFIPSMPLQMVLAQQTARALALGRGQELSGLIRAAWL
                     GTFLLWLAAVAGVWFFQGPILAQWKITNPAALWLTMPVLLFTVWLPMFYGVLQGQQNF
                     LWMGWSMISNGVGRLTVAAFAVLALQYRAPGMMMGMLGGMAAALVIAIWPTRALWLAP
                     PRSFDWRRLLRQVIPLMLGFGVYQFLLTADTMFVKAWFDEETSAFYVSAGTLARASMW
                     LVGPLAAVMFPKLVHASARAEKTNLLGVVLAGTAILAGGGALGLWLLGPWVVRFMFKP
                     SYVAAAATLLPWYAWAVVPLAMANVLLNHLLAHSRFKVVPALCALVVVYVAALTQFHD
                     SPVTIIKTLTVCNTLLLLICAWYTWGVKATEPEATSRPAA"
     gene            complement(6822..7949)
                     /locus_tag="PKI07_08180"
     CDS             complement(6822..7949)
                     /locus_tag="PKI07_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC51068.1"
                     /translation="MTKTERLLRELIALPSVNPAFLPARDGRAGEQRVADFLAVTAAR
                     AGLDVEFQVVARGRANVLARLSPRGRARQRILLAPHLDTVGAADEQFRPVTRQGRLFG
                     RGACDTKGSAAAMLMALCELAQSRQRPAATEIVFAGLVDEEHHQAGARALVAGGGRAD
                     LALVGEPTGLRAATAHKGTLWLRLETRGKSAHGARPELGRNAVHAMARVVELLQTTYA
                     AQLRRRRHPLLGCATVNVGAIQGGQQPNIVPDHCHILVDRRTLPGETDSGVRRELAGL
                     LRRKNLPAALGPDPQPPCPPLATDPAHPLAARFLRSLGQRRPVGVPYFCDAAVLARGG
                     IPSVVFGPGNIAQAHTAREWISLASLERGRELLLRFLRSLP"
     gene            8231..9577
                     /gene="thrC"
                     /locus_tag="PKI07_08185"
     CDS             8231..9577
                     /gene="thrC"
                     /locus_tag="PKI07_08185"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOC51069.1"
                     /translation="MSYRAWFQCINPQCGATYPLNSIVYQCQQCRALLEVQHDLAALS
                     RRGAAAWMKLFEDRYKSTAWPYGSGVWGKKEWILPQIRDENIVSLYEGGTNLFWAERF
                     GRVIGVEDLWIKLCGNTHSGSFKDLGMTVLVSQVKQMISEGAPIQAVACASTGDTSAA
                     LATYCAAAGIPSVVLLPRDKISLAQLIQPVANDALVLYLDTDFDGCMKLVQEITKDPT
                     LYLANSMNSLRVEGQKTVGIEIVQQFDWEVPDVIIIPGGNLGNVSALGKGLLLMRDLG
                     LIATLPRIVVAQAQRANPLYLAYQRNFETFEPVQARKTLASAIQIGNPVSFEKAVRTL
                     RQFNGIVEQASEEELADAAALGDRTGMFNCPHTGVALAVLLKLLKAGQINKTDRVVVI
                     STAHGLKFVEMKVKYHQRQLEFPCHHANEPVELPARLDAVKEALHKALQTKRQFRR"
     gene            complement(9675..10616)
                     /locus_tag="PKI07_08190"
     CDS             complement(9675..10616)
                     /locus_tag="PKI07_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51070.1"
                     /translation="MVTQLMADNNLASGTTNSATVSLLTTNDAVEQEFQRLMAADDAA
                     QAEVDQWIRDNNAAAARGSAVPKADLERRIRERFRPVREAYNDFLMRHPKHARARLAY
                     GSFLSDFHDNEGAREQWEKALALNPQDPAAYNNLAHLYSQTGPVTRAFEYYGRAIELS
                     PQQPLYYRNLGDAIYLYRTQAMEYYHLDEQQIYDKVLELYRKALSLDPTDFPLASDVA
                     QVYYGFKPLRLEAALRSWTNALALARDEIERQGVYLHFARLKLQTNRFEEVRAHLSAV
                     TNEMYAELKQRLTRNLEEREQAARGTNAPPAAAARQP"
     gene            11340..13283
                     /locus_tag="PKI07_08195"
     CDS             11340..13283
                     /locus_tag="PKI07_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOC51071.1"
                     /translation="MFVYPKQYDVIVIGAGHAGIEAALAAARLGCQTLLLTINLDTIG
                     QLSCNPAIGGLGKGHIAREIDALGGEMAKATDMTGLQFRMLNRKKGPAVWAPRAQCDK
                     KAYQFRMKWICERQPNLDLRQAQCSKVLQRDGQAYGVETTMEVQYHGKTIVLTTGTFL
                     NGLIHIGQNHQSGGRFGEPAVTGLSASLKEIGLELGRLKTGTPPRLLRRSINFDKIEV
                     QSGDEPVPYFTNWKEDLFHVEQSEAANNRKLFGWDRDQKFHVEHFNKYPPESILDKIG
                     SQIPCHITYTTKSTAEVIRANLHHSPLYSGKIHGIGTRYCPSIEDKIVRFAEKERHQI
                     FLEPEGATTEEIYVNGFSTSLPFEVQVQMVRTIIGCENAEILRPAYAIEYDFCFPNQL
                     RPSLETKVCQNLYLAGQINGTSGYEEAAGQGLMAGINAARRVNGLDPVVLRRDQAYIG
                     VLIDDLVTKDTTEPYRIFTSRAEHRLILRQDNADERLSKLGFEIGLLPKRHYLESKAR
                     QEAVAAEIARLRQTWSGSRTLAQLLCQPECSYRDIISIDVNLSDDIILRVETDIKYAG
                     YIARQEEEVARLKNLEDKQIPDGFDYQAVPSLRTEARQKLLKIRPATLGQAARIAGVS
                     PADIGILMVWLKRGSTPSRVGKA"
     gene            13602..14594
                     /locus_tag="PKI07_08200"
     CDS             13602..14594
                     /locus_tag="PKI07_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HOC51072.1"
                     /translation="MKAAFITQTGPPEVITYGDLPQPKPGRRQCLIKVAAVDVNPIDV
                     YVRSGAIPMKLSFPFILGRDLAGTVVEAGAGVRGYKPGDRVWASNQGAEGRPGTFAEY
                     AAVDYRWLNLIPEGVSDEDIVSLSLTGITAQLGLVRNAALRPREILFVNGGTGGVGAC
                     VVQMAKILGARVIATAGTDEKVAACRDLGADLAINYRTQDVVAAVRDFAPEGVNVWWE
                     TLREPDFDRAIPLLALRGRMVIMAGREARPPFPVGPFYVKDCSLHGLAVFNSSAREQR
                     AAANAINRWVAEGKLRARIDRVLPLEQAAAAHRLQEESTVQKTGALAGKLVLRP"
     gene            complement(15018..17252)
                     /locus_tag="PKI07_08205"
     CDS             complement(15018..17252)
                     /locus_tag="PKI07_08205"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51073.1"
                     /translation="MALAQFSAAAAGPAAAPAAAAGFAGSRSCRECHAKFYELWSTSF
                     HGLAMQPYTAELARSKLTPQAQEVVAGPYRFRADLERGVVIERGPEGEASFPMVQVMG
                     GKNVFYFLTPLERGKLQVLPVAYDVRRREWLDTTASAIRHFGDRHDEALYWKERPLTF
                     NTACFSCHVSQLEQNYDLASDRYHTTWAEPGINCETCHGPSAAHVELFRRWPTNQPPP
                     ADLKLIVTRALTTEQRNDSCAPCHAKMSPLTLSFAPGDRYFDHFDLVTLEHPDFYPDG
                     RDLGENYTFTTWRLSPCAKSGKLDCIHCHTSSGRYRFADPAKANHACLPCHAARVGEA
                     PAHTHHPPGSTGNVCIACHMPMTEFARMRRSDHSMRPPTPAATRQYQSPNACNLCHTN
                     QDAAWADRLVRQWHERDYQRPVLERAALIAAARKQDWKRLPDMLAELRRPDREEIQTA
                     SLVRLLADCPSEEKWPALRNLMPDPSPLVRACVAEALGQQLDQPNLAALVKATSDDFR
                     LVRVRAAAALAGIPEESLPERQRPAVNRAWRELIASMRARPDDMASHYNLGNFYLARG
                     QMREAVQEFETALRLEPDALPPQVNAALAYNALGQNDKAEARLRQALRLAPTNAAVNL
                     NLGMLLAEVGKLAEAEQAFRAAFKADPQSGQAAYNLGVLLAKEHPQEALEWCRRAAEL
                     RPENPQYGYTHAFYLHRAGQRDQALAVLRAVRQRHPDHQDSRRLEEQLLRERDAQRAG
                     SPDR"
     gene            complement(17519..18814)
                     /locus_tag="PKI07_08210"
     CDS             complement(17519..18814)
                     /locus_tag="PKI07_08210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 domain-containing metallophosphoesterase
                     family protein"
                     /protein_id="HOC51074.1"
                     /translation="MIKLLAALLPVLTRTSSAHARRRAAAAARAGLFGAGALAVGWAL
                     AAASAARAQNPPPAAAGSNAPAAFAITHGPFLQAPSPTGITISWATSRKGVSRVEYRL
                     ESATQWQTNFPAHHGLIDADVTTHNVALAGLEPGTAYRYRVVSREIVEFKPYQVTYGA
                     TIASPEYGFTTFDPRKPAAAFVVLNDRHEKVEPLAASLAAVDWARVGLVFFNGDAVNS
                     VESESQLYRCLVDPIVPPFATRIPLVYVRGNHDTRGKFARQLLNYFPTDSGRYYYTLQ
                     EGPVMFLVLDGGEDKADGSSEYSGLVDFDPYLHREAEWLARQIQDPAFQRAPFRIGLL
                     HIPPANDPDPKFIRRKWLQDHLVPILNRGQLDLLICGHMHKYSLHPAGHEGRDFPLII
                     GGTETVIRGDVTREQIRLTTTDLSGRELPQLPPIQRRSR"
     gene            complement(18807..22013)
                     /gene="carB"
                     /locus_tag="PKI07_08215"
     CDS             complement(18807..22013)
                     /gene="carB"
                     /locus_tag="PKI07_08215"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOC51075.1"
                     /translation="MPRRTDIRKVLIIGSGPIVIGQACEFDYSGTQACKALRSLGFQV
                     VLVNSNPATIMTDPGMADATYIEPLTVAALTEIIEKERPDALLPNLGGQTGLNLSSQL
                     AKAGVLAKYGVRVIGVEADAIEKGEDRIIFKETMKKLGIEMPRSAPAFSVEEAERVAA
                     ELGYPLVIRPAYTMGGTGGGHVYNVEELRLVASRGLDASIVGQILVEESVVGWEELEL
                     EVVRDAKNQMITACFIENVDAMGVHTGDSYCVAPMLTIDPRLQARLQELAYRIVEAIG
                     VIGGTNVQFAHDPRTGRVVIIEINPRTSRSSALASKATGLPIALISAKLAAGLTLDEI
                     PYWRDGTLEKYTPSGDYVVVKFPRWAFEKFKGSEDKLGTQMRSVGEVMSIGKTYKEAF
                     QKAIRSLENGRYGLGFARDYHRKPLEELMLLLNEPSSERQWIMYEALRKGAAVDELYR
                     KTHIKPWFIQQMKELVDLEEQILKHQGKLPPDSLLIQAKKDGFADRYLAKLLGLPEAR
                     IREQRKKLGVTEAWDALPVSGVEKAAYYYSTYNAPDRVATTPRKKVMVLGGGPNRIGQ
                     GIEFDYCCVHAAFTLRDAGYETIMVNCNPETVSTDYDTSDKLYFEPLTLEDVLAIYEK
                     EKPEGVVVQFGGQTPLNLANDLAAAGVRILGTTPDMIDLAEDRDRFRKMMEQLDIPMP
                     TSGMASSFAEARRIAEQIGYPVMVRPSFVLGGRGMEIVHDEEMLRHYIHAAVEVTPER
                     PILIDRFLDNAVEAEADALADGQDAFIPAIMEHIELAGIHSGDSACAIPPVTLPRKHI
                     KTIEEYTLRISRELKVCGVMNIQYAVHQDKVYVLEANPRASRTVPLVSKVCNIPMAAI
                     ATRLMLGQRLRDLDLRHRPIRHTGVKEVVLPFNMFPEVDPVLGPEMRSTGEVLGMAPT
                     FDLAYFKSQEAAGSPLPLKGTVFISVTDKDKPVMLPTARRFAELGFRLKATHSTFKFL
                     QANGIPCEISFKISEHYRPNIADEIKSKQIDLVINTPRGKTGAADGAAIRKAAIKYKI
                     PYVTTTAAAKAAVAGIAAARTGRAGVKSLQGYHQDIHD"
BASE COUNT         4220 a         7177 c         7350 g         4118 t
ORIGIN      
        1 gttgccgtgc gtcgtggttt ttgggcggga tggggtcacg gctggcgggg acatttcccc
       61 tcccgggtgg gggttgccga aaggcggctg cttgcgttgt cctcatcgtg ggcggagtat
      121 tcgactgatt ggggtgagtt tggttgaaga tttgttccca ggctatgcgc gccgggtgaa
      181 tgattgacgg gatgggttca ggaacctgtt caggggcgtt ggttgtgtgg atcaggagcc
      241 ggggagattg gggcggcggg ttgggcggcg gttcagcgct tcgttcgggc tccgcccctg
      301 ccacttcgcc tgggataacc tccctcgcac gccaactccc gcgtccaacc ttttatatct
      361 attcctccgc gtcagccgcc ccgctgccgc tgagaacccc gtccagagtt caagaaagca
      421 gcccaagcct ccgcatcagc cactccgctg ccactgagaa ccccgtcagg ggttcaagaa
      481 agtagcccag ggtcagcgag gaacgagcgc caccctgggt aatccacggc gagaaataca
      541 tcccccctct cctccgccgc ggaggagagg gggaaggggg agaggtggcc aacaccgcac
      601 acgcgccccc ccggccgccg cgcagactcg gccgccagtg ccaccccgcc gcaaagaacc
      661 cgcgcgcgca ggggcgcaac ggagaacccc gtccggggtt caagaaagca gcccaggatt
      721 tgaacagtct ggttcctgaa gctcgcgcca ggttctgggc ggaaattgaa ggcaaggtgg
      781 acagtcagga taagcgaacc ccatccggca agcagtccca tcgctaaacc tcgctccgcg
      841 gaaagccaga actacgacgc ctcgcgcttc gcgcccgccg tgaaaaccgc cgagacaggc
      901 ggtcttcccc ggcgacgtga gagcggccag cttcggctac ggttacgatg ctcgtgcctg
      961 tgcgtccggg ccttcgcaga actggccgcg ctcccgccgc ccccaacgca gaactcttcg
     1021 ttcggagggg gtgtcttgcc gcagggcaac ggcttcgagg tttggacccc cctttttccc
     1081 ctcagctgcc caaactcagg cgccgctaac caaagaccct ggccagccgg ttttccgggc
     1141 cctcgaatcg ccgtcagtcc aacccggccg cggcgggaat cgtcgggaag ggttgattag
     1201 ggtgaaacct tcggcgtcat gaatgcgtct taaacgacag cgagttgctt gagttaaaca
     1261 acggtcatgc taccctcagc catcatgggt gccacttcgc cagccgtgcg ccagcgcggg
     1321 aaagttgcca aaccgccgcg cggcggcgcg cggcccgcgc catcgcgccg ggttcgaacg
     1381 caagccggcc ccggcccggc ggcggccgtc cggcaagcgc aggtggcgca gggccaggcc
     1441 acgcacctgg ccctgatggt ttcccccacg gaacgcggca cgtttcgctc gcaggcccgc
     1501 gcggtctggt cggccctgcg ggcggcgctc gaacagccgc ccggaacgaa ggccatcacg
     1561 gcgctgacgg ttttcctgaa ggatccccgc cagcagcggg aatgcgagcg ggtgtgcgcg
     1621 gatgggttgg gcccggaacg gccgttcatc aattacgttt ggcagccgcc ctgcgatggc
     1681 gcggcgctgg ccctcgaggc ctgggccatt ggcggcccgg cggtgcgctg cgaacgccgc
     1741 ggcccgcacg cgctcgtggt ggattgcgat ggcgtccgct gggtttattg cgccggcatc
     1801 caggcggcgg cgcgcggggt ttatcgccag acgctggcgg cgctggcgca gtcgcgcgcg
     1861 gcgctggccc gcgccggcag ccgtttcgag cgggtggtcc gcacctggtt ttacctgggc
     1921 ggcattaccg agccggaggc ggagacgtgg cgctaccagg agttgaaccg cgcccggacg
     1981 gatttctacc gcgcggttcg ctttcacggc gcggcgccgg accccggcgc ggccgccggc
     2041 atttatccgg ccagcacggg catcgggatg cggggccgga agctggccct gagttgcctg
     2101 gcgctggaga cccggcggag cgatgttcat ttgcgggctt tggaaaatcc gcggcaaatc
     2161 cctgcctacg cctacgacgc ccgctactcg ccccagagtc ccaagttctc gcgcgcggtg
     2221 gcgctgcggg tgggcgacca cgtgaccacc tggatttccg gcaccgccag catcgtcaat
     2281 tcggaaaccc gccacccgga cggcgtcgag catcagaccg agcagacgat tgacaatatc
     2341 gagcggttga ttgccgccga taacttcgcc cggcacgggc tggcgggcgc gggggcccgc
     2401 ctgcgtgacc tggccgggat tcgcgtgtat ctcaagcgcc cggcggattg gaaacggtgc
     2461 cgggccattt gcgagcggcg cttcgggccg gtgcccgccc tgtacgtggt ggccgacatc
     2521 tgccggccgg agctgcgggt cgaaatcgag ggcgtggcct tctcccggct gggcgcgccc
     2581 ccggcgccgc gcgaggttgc ttagccggcc ggttccgcct aaaatgcggg gcgtgcggct
     2641 gcaacccttg agattcgatc ccggcttatg tccaaaagcg aaacgatgac cccgcgcgag
     2701 cgggtcctga aagcgctcaa ccaccaaatc ccggaccgcg tgccgattga cctcggcggc
     2761 aaccagaccg gcatccaccg gaacgcctac cggtcgctgc tccagcacct gggcgtgacg
     2821 gacgaaatcc gcatcatgga cgccgtccag cagctggccg cgcccggcga gcaggtcctg
     2881 cagcggttcc atgtggatac gcgctacgtg gcggccgggg cggcggccag ttggaagggc
     2941 ggcatcgtgc cggcccggcg cgacgggcgc aactgggacg atctgacgga tgagtttggc
     3001 gtccgctggt ccatgcccga ggatgcgccc cactacatgg acatcaccct gcacccgctg
     3061 gccaacgcga ccctggccga gcttcgggat tacccgtggc cccggggcga tgatcccagc
     3121 cggtttgccg gcttgcgcga gcgggcgctg acgttgaagc gggaaacgcc gtatgccgtg
     3181 gtcagcggca tctccggggt ggtgtatgag atttgctggt atctgcgcgg gctggaacaa
     3241 tggttctgcg acctgatgta tcagccggag ttctgcgcgg cgatgctgga tcagacgctc
     3301 aagttctggc tggactggtt ccgtctgttc ctcgacgagg tgggcgacgt ggtggatgtc
     3361 atcatgatcg gcgacgacct ggccggtcaa aacgggccgt tgttcaaccc ggcgatctac
     3421 cgtcagatcg tcaagccccg ccaccagcgc ctggtgcaat acctgcgctc ccgcaccgcg
     3481 gcgaaaatct ggtatcatac ctgcggcgcc tgccaggact tcattcccga cctcatcgag
     3541 aacggcgtgc aggtgctcaa cccggtgcag gtcagcgcgc gcaatatgga tccggcggag
     3601 ctgaagcgcc gctttggacg ccaaatcgcc ttttggggcg ggggcgtgga cgcccagcat
     3661 gtcctgtcgc gcggcacgcc ccaggaagtg gccgccaacg tccggcagaa cctccaggcc
     3721 ctcatgcccg gcggcggcta tatcttcaac aacgtccaca atatccaggg cgaagtgccg
     3781 ccggagaatg tggttgcgct gttcgatacc gcctacgaac acggtttcta ctgagccgcc
     3841 cggcgtttcc ggaagcaacc gcccccaaac cggcggcggc gaaacggcgg cccggatccg
     3901 cgcccggaag accgcgccgc ctgggaaccg ggccatcgcc gggaaagtcc tcccgggtga
     3961 gggttgaccg gcttcggcgt ggccgggggt tggcattcag gctgctttcc caggaggcaa
     4021 actatgacag gcaaggctgc tttggttcgc atggccaggt tcgcgacgct ggccctggcg
     4081 ctgggaacgc ccgtactggc gttgtccggc gcggaagtaa cgctcaccca aaccaactgg
     4141 gtggaacgct ggatcaccaa cgtcatcgag gtgcggatgc ccctcaacca gttcgtcaat
     4201 gaataccgca ccaactgggt cacgcagttc cagaccaacg tcgttaacgt gttcgccacc
     4261 aaccgggtga cccggaccct gaccaatacg gtcgtggtgg atctggttca aaccaatttc
     4321 gtggtgggct accagaccaa ccgggttgtt cgcatgcaga ccaactatgc ggcggtcaac
     4381 cttttccaga ccaattttgt ggatcagtgc caaacaaatt ggagcgtgct gaccctgacc
     4441 aactgggaga cggtggttct gttccggacg aattgggtcg tgcagccggc caccaatgtg
     4501 gtccaaatcg aattgccgcc ctccccggcc ccggctgttg cggccgcccg cgaggccgcc
     4561 gcggcgccgg aacccccggc ggccgctccg gcgaaagcgc ccgcgcccgt cccgccgcct
     4621 gccgcctggg acggcccgct ggccctcgag gtggtccgca ccgcccggcc cgccgcccag
     4681 cacccgatcg aagtgctgct caaagtccgt tggacggata gcaccgccgc gccgccgccg
     4741 gtccagcagt ggcgaatcga gcgcgaggac gcggccattc tcctctttgg ccaggaacag
     4801 gaattcaaac ggcaactgcc gccgggcaaa tacaaagtcg aagcccggct caaggccgcc
     4861 ggcgacgatc ttccgttttc cgtgcgcggc atcctggccg tcaccaccac tgacgcggtc
     4921 atccagccgc gcttgctggt gcaaaagtag attcctcgtt ctgattcggc gaggcggcaa
     4981 aacccgccgt ccgcgccgac acccgggcgc aacggggtcg aggcggggag cacggggtgc
     5041 gatggcgccg ggaggggaac gccggttgtc cgcgcgattc gctggttgag ccgtccattt
     5101 tcaagccgga tccggccgcg gcaacggttg gcgcgcgtta tgctccggca gactcaatac
     5161 ggcaaaccaa acctgtgcta tgaaaacatt cttaagagac gtggcgaccc gccaatactt
     5221 ccgttccgcc gatcattgga cttttgatcg ggaagaggcg catgacttcg gttttattcc
     5281 ccaggccatg cgaattgcgc gcaagctggg ggtgtccacg ctggaagtgg tcgtttcctt
     5341 cgaggattcc cttccgctgg cgaatatgtc gctggcggac ttcctgcgtg gattatcgcg
     5401 tccgggacgg cgccggacgg cgggcagccg ggcctgactg gccgcggggc acggctgtaa
     5461 agatctgagg cactccgcgc cgggcgggcg cgctttaagc ggccgggcgg ctggtggctt
     5521 ccggttccgt ggctttgacg ccccaggtgt accaggcgca aatgagcagc agcagcgtat
     5581 tgcacacggt cagcgtcttg atgatggtga cgggggagtc gtggaactgc gtgagggcgg
     5641 cgacgtagac cacgaccagg gcgcagagcg ccgggaccac tttgaaccgg gaatgggcca
     5701 gcaggtggtt cagcagcacg ttggccatcg ccagcggcac caccgcccag gcataccagg
     5761 gcagcagcgt tgccgccgcc gccacgtagc tgggcttgaa cataaatcgc accacccagg
     5821 gccccagcaa ccacaacccg agcgcgccgc cccccgcaag aattgcggtt ccggccagca
     5881 ccacccccag caggttcgtt ttttcggccc gggcgctggc gtggacgagc ttgggaaaca
     5941 tcaccgccgc cagcggcccg accagccaca ttgaagcccg cgccagcgtc cccgcgctca
     6001 cataaaaggc ggaggtctcc tcgtcgaacc aggccttgac aaacatggtg tccgccgtga
     6061 gcaggaattg atagacgcca aaccccagca tcaggggaat gacctggcgc agcaagcggc
     6121 gccagtcaaa cgaccggggc ggcgccagcc agagcgcgcg ggtgggccaa atggcaatta
     6181 ccagggccgc cgccatgcca cccagcatgc ccatcatcat ccccggcgcg cggtattgca
     6241 gcgccagaac ggcaaaggcg gcaacggtca ggcgccccac cccgtttgaa atcatgctcc
     6301 accccatcca caggaagttt tgctgccctt gcagcacgcc gtaaaacatc ggcagccaga
     6361 cggtgaacag cagcaccggc atcgtcagcc acaacgcggc cgggttggtg atcttccact
     6421 gcgccagaat gggcccctgg aagaaccaga cccccgccac cgccgccagc cacagcaaaa
     6481 acgttcccag ccaggcggcg cgaatcaggc ccgacagctc ctgcccgcgg cccagcgcca
     6541 gcgcccgcgc ggtttgttgc gccagcacca tttgcagcgg catggacggg atgaacatcg
     6601 tcaccgcgag gaacaccccg aactgggcat actccgcctc cggcaacgcc cgggctttcg
     6661 agaggaagtg cattgccgtc atgaaaaggc cgccggcgat ggtggcaaac atgagccagc
     6721 cgctctgacg gaaaaaggag gcccggtgcg cggcggtgtt cgcgggcggg gtttgggcag
     6781 gttggtcgcg catggaacgg gaaaaggggc gggaatccgg gtcagggaag cgaacgcaaa
     6841 aaacgcaaca gcagctcccg gcccctttcc agggatgcca gggaaatcca ttcccgcgcc
     6901 gtgtgcgcct gcgcgatgtt gcccgggcca aacaccacgc tggggatgcc gccgcgcgcc
     6961 agcaccgcgg cgtcgcaaaa ataaggcacg cccaccggcc gccgctgccc caggctgcgc
     7021 aaaaaccggg cggccagggg atgtgccggg tccgtcgcca gcggcggaca gggcggctgg
     7081 ggatccggcc cgagcgccgc cggcaggttc ttccggcgca acagcccggc cagttcgcgc
     7141 cggacgccgg aatcggtttc tcccggcagc gtgcggcggt ccaccaggat atggcaatga
     7201 tcgggcacaa tgttgggctg ctgcccgccc tggatggcgc cgacgttcac cgtggcgcag
     7261 cccagcagcg gatgccggcg gcggcgcaac tgcgcggcgt aggtcgtttg cagcagctca
     7321 accacgcggg ccatggcatg caccgcgttg cggcccagtt ccggccgggc gccgtgcgcg
     7381 gatttgcccc gcgtctccag ccgcagccac agggtgccct tgtgcgcggt tgccgcccgc
     7441 aaaccggtgg gctcgcccac cagcgccagg tcggcccgcc cgccgcccgc caccagcgcg
     7501 cgcgccccgg cctggtggtg ctcttcatcc accagccccg caaaaacaat ttccgtcgcc
     7561 gcgggacgct gccggctttg cgccagctcg cagagcgcca tgagcatggc ggccgccgag
     7621 cctttggtat cgcaggcgcc acggccaaag agacggccct ggcgcgtcac gggccggaac
     7681 tgctcatcgg cggcgcccac cgtgtccaaa tgcggcgcca gcaaaatccg ttgccgcgcc
     7741 cggccccggg gcgaaagccg cgccagcaca ttggcccgcc cgcgcgccac gacctggaat
     7801 tccacatcca gacccgcccg ggccgccgtc accgccagaa aatcagccac ccgctgctcg
     7861 ccggcgcgcc cgtcccgcgc cggcaaaaaa gccgggttca cgctcggcag ggcgatcagc
     7921 tcgcgcagca atcgttcggt tttcgtcatg cggtttcccg aggaaaatca gtcgtccagc
     7981 ttaggcggtt tccccgcccg ctgtcgagca cttcgccgcc ggcgcgggtt gggcggcgcc
     8041 cgcgcgcggc gggcggaccc gttgaagcag gcaccgccgg cccgtccggc cgcgcgcggg
     8101 ggagaggcag ggcgcgttct ccgtcattcc gcgcttgaat tgcccgggca aactggcatc
     8161 ctgccggggc ccttgagtga tgacgccgcc ggggcgggct gtccgctgcc gcgcaaaaga
     8221 gaataagaac atgtcctatc gagcctggtt ccagtgcatc aacccgcaat gcggggccac
     8281 ctatccgctg aactccatcg tgtaccaatg ccagcaatgc cgcgcgctgc tggaggtgca
     8341 gcacgacctc gcggcgttgt cccggcgcgg cgcggcggcg tggatgaaac tttttgaaga
     8401 ccgttacaag agcaccgcgt ggccgtacgg ctccggggtt tggggcaaga aagaatggat
     8461 cctgccgcaa atccgcgatg aaaacatagt ctcgctctac gaaggcggca ccaacctgtt
     8521 ctgggcggaa cgcttcgggc gggtgattgg agtggaagac ctctggatca agctgtgcgg
     8581 caacacccac agcgggtcgt tcaaggattt gggcatgacg gtcctggtct cgcaggtcaa
     8641 gcagatgatt tccgagggcg cgccgatcca ggcggtggcc tgcgcctcga cgggcgacac
     8701 ttcggcggcg ctggccacgt attgcgccgc ggcgggcatc ccgtccgtgg tgctgctgcc
     8761 ccgggataaa atctccctcg cgcagttgat ccagcccgtc gccaacgacg ccctggtgct
     8821 gtacctggac acggattttg acggctgcat gaagctggtg caggagatca ccaaagaccc
     8881 gacgctgtac ctggccaatt cgatgaactc gctgcgcgtg gagggccaga aaaccgtggg
     8941 catcgagatc gtgcagcaat ttgactggga ggtgcccgac gtcatcatca ttcccggcgg
     9001 caacctgggc aacgtctccg ccctgggcaa agggctgctg ctgatgcgcg acctggggct
     9061 gatcgcaacg ctgccgcgca ttgtggtggc gcaggcgcag cgcgccaacc cgctttacct
     9121 cgcctaccag cgcaacttcg agacctttga gccggttcag gcccggaaaa ccctggccag
     9181 cgcgatccag atcggcaacc cggtcagctt cgagaaggcc gttcgcacgc tccggcagtt
     9241 caatggcatc gtcgaacagg ccagcgagga agagctggcg gacgccgcgg ccctgggcga
     9301 ccgcaccggc atgttcaact gtccgcatac cggcgtcgcg ctggcggtgc tgctcaaact
     9361 gctcaaggcc ggccaaatca ataagactga ccgcgtggtg gtgatttcca ccgcgcatgg
     9421 ccttaaattc gtcgagatga aggtgaagta tcaccagcgc cagcttgaat tcccctgtca
     9481 ccacgccaac gagcctgtcg aattgcctgc gcggctggac gccgtcaaag aagccctgca
     9541 taaggcgctc cagaccaaac ggcagttccg gcggtagcgt ctccgcgccc gccgggcggg
     9601 cattttgcct gcgtgcggtg cctggctgct gaacgcgggc ccgcgattcg ccctctgaaa
     9661 ccgggaaagg cgcgttatgg ctgccgggcg gcggctgccg gcggtgcgtt ggttccccgc
     9721 gccgcctgct cgcgttcctc cagattgcgc gtcaggcgct gcttcaactc ggcgtacatc
     9781 tcgttggtga cggcgctgag atgggcgcgg acttcctcga agcggttggt ttggagtttg
     9841 agccgggcga agtgcaaata tacaccctgg cgttcgattt catcccgggc tagcgcgagg
     9901 gcattggtcc aggaacgcag tgccgcttcg aggcggagcg gtttaaagcc gtagtaaacc
     9961 tgggccacat ctgaggcgag cgggaaatcg gtcggatcca ggctcagggc tttgcggtag
    10021 agttccagca ctttgtcata aatctgctgc tcgtcgaggt ggtaatattc catggcctgc
    10081 gtgcgataca ggtaaatggc gtcgcccagg ttgcggtagt agagcggttg ctgcggactt
    10141 aactcgatgg cccggccata atactcgaag gcccgggtga ccggaccggt ctggctgtaa
    10201 agatgggcaa ggttgttata ggccgccgga tcctggggat tgagtgccag cgccttttcc
    10261 cactgctcgc gcgcgccctc gttatcgtga aaatcgctca gaaagctgcc ataggccagc
    10321 cgggcgcggg cgtgcttcgg gtgccgcatc agaaaatcat tataggcctc gcgcacggga
    10381 cgaaaacgct cacgaatccg ccgctccaaa tcggctttcg gcacggcact gccacgagcg
    10441 gccgccgcgt tattatcgcg aatccactgg tccacctcgg cttgcgccgc atcgtccgcc
    10501 gccatcaggc gctgaaactc ttgctcaacc gcatcgttcg ttgtcagtaa cgaaactgtg
    10561 gcggaattgg tagttcctga agcaaggttg ttatccgcca tcaattgcgt gaccatcccg
    10621 cagatgccaa gcaagctaat aaagattatc cgcacaccaa acgctaacat gacgctccga
    10681 aaaagcaaag ggcgaactca aaaccgtgat tacagcaggt tgactcgatg agcaggtaag
    10741 gcatattagt gtatagttat cgttaacggt agcgaataaa taaccatttc ggactgattt
    10801 ctaactgttt ttgagcgtat tttataattc tgcgttagtt tttaacagct tgggatagcg
    10861 cgacaaagcg cggcttgtac tgggttccaa aatcacgacc atatagagaa acaaggtcat
    10921 gaggccgatc attcgcacac gacgatactt tctgacacct caaaaccaag gctaaaacag
    10981 gtatccgtcc accagaacgg gacttgccag atgttaacga aaaaagttgg tgttgcgtag
    11041 aaacaagcgt aagtatattg aatccttcgg catagtaaga caattatcag gcacgaaaca
    11101 acctgggaac acgcgcggaa agagaatgaa gctcgaaaag taagactcgt agctcgccga
    11161 acattcagaa ccaaataatt tacggcccag ctaaatacga cgatcgcaga atgagtcaat
    11221 ttaagggtat tttagacaga aatagggatg tttttacctg ctttatgcga attttcagct
    11281 atgttatcgt taacatagtc tgacggttga cttgctaatt ccgttgcttt taaggagtca
    11341 tgttcgttta tccaaagcaa tatgatgtta tagtaatcgg agctggacat gccggaatcg
    11401 aggctgcact ggcggcagct cgtttgggtt gccaaacgtt gctgctaacc atcaatctcg
    11461 atacgattgg gcaattatcg tgcaatcctg ccatcggcgg ccttggaaaa ggacatatcg
    11521 cccgtgaaat agatgcgctg ggcggtgaga tggcaaaggc gaccgatatg accgggttgc
    11581 agtttcggat gctaaaccgt aaaaaagggc cagcggtttg ggcgcccagg gcgcagtgcg
    11641 acaagaaagc ctatcaattt cggatgaagt ggatatgcga gcgacagcca aaccttgatc
    11701 ttcgacaggc acaatgctcc aaagttctgc agcgagatgg tcaagcctat ggcgtggaaa
    11761 cgaccatgga agtgcaatat catggcaaga cgattgtgct gacaacgggg acgtttctga
    11821 acggtttaat ccacatcggc cagaatcatc agtctggagg tcgttttggt gagccggccg
    11881 ttactgggct atcagcctcg ttaaaagaga ttgggcttga attagggcga ctcaagaccg
    11941 gaactcctcc acgattgctg cgccgctcga tcaattttga taagatcgaa gtccaatccg
    12001 gagacgaacc ggtcccctac tttaccaact ggaaagagga tttgttccac gtggaacaat
    12061 cagaggctgc caacaaccga aagctattcg gatgggatag agaccaaaag ttccacgtgg
    12121 aacacttcaa taaatacccg ccggaatcaa ttctcgataa aatcggaagc cagattcctt
    12181 gtcatatcac ctataccacc aagtccaccg ccgaggtgat tcgggctaat ttacaccatt
    12241 caccactgta ttccggtaaa atacacggga ttggcacccg atattgtccg tccattgaag
    12301 ataaaatcgt ccggttcgcc gagaaagagc gtcaccagat attcctggag cctgaaggtg
    12361 ctactaccga agagatttac gtaaacggct tttcaacttc gctcccgttc gaggtacaag
    12421 tgcagatggt gcggacgata atcggatgcg aaaatgccga aatcctaaga cccgcatacg
    12481 ctatcgagta tgacttctgc tttcccaatc agcttcgtcc gtcactggaa accaaagttt
    12541 gccaaaatct ttatctagcc ggccaaatca atggaacgtc cggatacgaa gaagctgcgg
    12601 gtcagggact catggccggt attaacgcag cccgtcgggt taatgggctt gatcctgtgg
    12661 tattgcgtag agaccaggct tacatcggcg tattgattga tgatttggtg acaaaagaca
    12721 cgaccgagcc gtaccgtata ttcacgtccc gggcagagca tcggctgatt ttgcggcagg
    12781 ataacgccga cgaacgatta tcaaaactgg gtttcgagat cggacttctg ccaaaacgtc
    12841 attacctaga atccaaagcc cggcaggaag ccgtcgccgc cgaaatagcg cgattgcggc
    12901 agacctggag cgggtcgcgc accctggccc agttgctatg ccagcctgaa tgttcatatc
    12961 gagatattat atctattgat gttaatttat cagacgatat aatacttaga gtcgagactg
    13021 acattaaata cgccggttat atcgctcgtc aggaggagga agtagccagg cttaagaacc
    13081 tggaagacaa gcagatccct gatggttttg attaccaggc ggtgccgagc ctgcgcactg
    13141 aggctcggca aaagctgttg aaaattcgcc ccgcaacgtt aggccaggcg gcccgcattg
    13201 ccggggtttc gccggcggac atcgggattc tcatggtttg gctgaagcgt ggcagcacgc
    13261 cgtcgcgcgt cggcaaagct tgagttggga tgcgggtata aaggttcgcc ttgagacgga
    13321 aatccgccgc tgcatttccc accaaactcg ccaaatacgt aaaaagatga ggcccaggca
    13381 tggagcgtga cgcataacgc ctgattgcgc ccggaataaa aatgacgccc gccgaaaatc
    13441 gcgcagcgcg tctcacgctc caaaagccgt taatgcacgg aactccgcat cgcaaacccg
    13501 cctgccgccg cccacgcccg atcgtgtgat gacgcaccct gcgcgcgctt gcggcccggg
    13561 gagcgcggtt ctacattggc ggctggcaaa tatcgaactt tatgaaagct gcatttatta
    13621 cgcaaacggg gccgcccgaa gtcatcacct acggcgatct tccccagccc aaacctggac
    13681 gccgacaatg cctgatcaag gttgccgcgg tggacgtaaa cccgattgac gtttacgtgc
    13741 ggagcggggc catcccgatg aagttgtcgt ttcccttcat tctcgggcgc gacctggcgg
    13801 gcacggtggt ggaggcgggt gccggggtcc ggggctataa gccgggagat cgggtttggg
    13861 cctccaacca gggcgcggaa gggcgtccgg gcacctttgc cgagtatgcc gcggtggatt
    13921 atcgctggct gaatctcatt cccgagggag ttagcgatga ggatattgtc tcgctctcac
    13981 tgacgggcat caccgcccag cttggcctgg tgcgcaacgc tgctttgcgg ccccgggaga
    14041 ttctctttgt gaacggtggc actggcggcg tgggcgcctg cgtggtgcag atggccaaaa
    14101 tcctcggcgc gcgcgtcatt gccacggcgg gcacggatga aaaagtcgcg gcctgccggg
    14161 atttgggcgc tgacctggcc atcaactacc gaacccagga cgtggtggcg gccgtccggg
    14221 acttcgcgcc ggaaggcgtc aatgtctggt gggaaacgct gcgcgagcca gacttcgacc
    14281 gggcgattcc gctgctggcg ctgcggggcc gaatggtcat catggccggg cgggaggctc
    14341 gcccgccgtt cccggtcggg ccgttttatg tgaaagactg ctcgctgcac ggactggcgg
    14401 tgttcaactc cagcgcgcgc gagcaacgcg cggcggccaa cgccatcaat cgctgggtgg
    14461 cggaaggcaa attgcgggcg cgcattgacc gggtgctgcc gctggaacag gccgccgccg
    14521 cgcatcggtt gcaggaagaa agcacggtgc agaagaccgg agcgctggcc ggcaaactgg
    14581 tgctccggcc ttagcccgca tccttcactt ccaacgccgc cggcaggcgc gcgcctgaaa
    14641 cgcagcccgg gtttcctaga gccgcgctcg ccaggctctg ccaatgggtc atggcttgcg
    14701 ctggattcat cgggaaaacc gcaatttcac ctcgctcccg aggacacaca gcaggttcaa
    14761 aacactgccc tcgccggagc gggctgcata aaaaaggcgc accaaacagg gtgcgcccga
    14821 atacacacgg tcactgatag tgtacccagc ggggcgaact tggcaacccg gtttttgaac
    14881 ctcattcgcc ctgggacgcg ttttgcagcg ctcctccttc ctcctccctg ttgccggctt
    14941 tcccgcgaac gcttttcctc tcccggggcg aaggatgccg tagcgtcgca cgcccccaag
    15001 gccagggcgt tttccggtca acggtccggg ctgccggcgc gttgcgcgtc ccgttcgcgg
    15061 agtaattgct cttcgagccg ccggctgtcc tggtgatcgg ggtgccgctg gcgcacggcg
    15121 cgcaggaccg ccagcgcctg gtcgcgctgt ccggcgcggt gcaggtaaaa ggcgtgcgtg
    15181 tagccatact gcggattttc gggccggagt tccgcggcgc ggcggcacca ctcgagcgct
    15241 tcctggggat gctcttttgc cagcaatacg cccaggttgt aggcggcctg gcctgattgg
    15301 gggtcggcct tgaacgcggc gcggaatgcc tgctcggctt cggccagctt gccgacttcg
    15361 gccagcaaca tgccgagatt gaggttgacc gcggcgttgg tgggagcgag gcggagcgcc
    15421 tggcgcaggc gggcttcggc tttatcgttc tggcccaggg cattgtaggc cagggcggca
    15481 ttgacctgcg gcggcagggc gtcgggctcc agtcgcaggg ccgtctcgaa ctcctgcacc
    15541 gcctcgcgca tctggccgcg ggccaggtag aagttgccaa ggttgtagtg cgacgccatg
    15601 tcgtccgggc gcgcgcgcat cgaggcgatc aactcgcgcc aggcgcggtt gacggcgggc
    15661 cgctggcgtt cgggcaggct ttcttccggg atgccggcca gggcggcggc ggcccgcacg
    15721 cgcacgaggc ggaagtcgtc gctggttgcc ttgaccaacg cggccaggtt gggttgatcc
    15781 aattgttgcc ccagcgcctc ggccacacag gcgcggacca ggggcgaggg atccggcatc
    15841 aggttccgca gggccggcca tttctcttcg gaagggcaat cggccaggag acggaccagc
    15901 gacgcggtct ggatttcctc gcggtcgggg cggcggagct cggccagcat atcgggcagc
    15961 cgcttccaat cctgtttgcg cgccgcggcg atcagcgcgg cgcgttcgag caccggccgc
    16021 tgataatcgc gctcgtgcca ctggcgcacc agccggtcgg cccacgcggc gtcctggttg
    16081 gtgtggcaaa gattgcaggc attgggggac tggtattggc gggtggccgc cggcgtcggc
    16141 gggcgcatcg agtgatcgga gcgccgcatg cgggcgaact cggtcatcgg catgtggcag
    16201 gcgatgcaga cattgccggt gctgccgggc gggtgatgag tgtgggcggg cgcctccccg
    16261 acccgggcgg cgtggcaggg gaggcaggcg tggttggcct tggccggatc cgcaaaccgg
    16321 tagcgcccgc tggaagtgtg gcagtggatg cagtccagct tgccggattt cgcgcagggg
    16381 ctgagccgcc aggtggtgaa ggtgtaattt tctcccaggt cccgtccgtc gggatagaaa
    16441 tccggatgct ccagcgtgac caggtcgaag tgatcaaagt agcggtcgcc gggggcgaag
    16501 ctgagggtca ggggcgacat tttggcgtgg cagggcgcgc aggagtcatt gcgctgctcc
    16561 gtcgtcagtg cgcgggtgac gatgagcttg aggtcggcgg ggggaggctg gttggtgggc
    16621 caccggcgaa acagctcgac gtgcgccgcc gaggggccgt ggcaggtctc gcagttaatg
    16681 cccggctcgg cccaggtggt gtggtagcgg tcggaagcca ggtcgtagtt ctgctcaagc
    16741 tggctgacgt ggcaactgaa gcacgccgtg ttgaaggtca gcgggcgctc cttccagtag
    16801 agcgcctcgt cgtggcggtc gccgaaatgg cgaattgcgc tggcggtggt atccagccat
    16861 tcccggcggc gcacgtcata agcgaccggc agcacctgca gcttgccccg ctccagcggc
    16921 gtgaggaaat agaacacatt cttgcctccc atcacctgca ccatcgggaa actcgcttcg
    16981 ccctccgggc cgcgctcaat caccaccccg cgctcgagat cggcccggaa ccggtaggga
    17041 cccgccacca cctcctgcgc ctgcggggtc agcttgcttc gggccagttc cgccgtgtag
    17101 ggctgcatgg ccaggccgtg aaacgacgtg gaccataatt cgtagaactt cgcgtggcat
    17161 tcgcggcaac tgcgggaccc ggcaaacccc gccgccgccg cgggcgccgc cgcgggaccg
    17221 gctgccgccg cgctgaactg cgccagggcc attcctcccc cgatcagccg ccaaccccaa
    17281 ctcccccaac gcgccgccgc gcgccgtccg gcgcgggttc tccccgcccg gcccggccac
    17341 tctttcgccg ggccttttct gcgaccaccg ggatttgggg cgctacgaca gaacatcccg
    17401 ggaatcctgc cggagccgga gactccgggc cccgcccggc ggcgccgggg ggcggaacgc
    17461 ttttttatcc gcggaggccg gtcgttcggg gcacaacatg agcgccgctc cgcgggggtt
    17521 accggctgcg ccgctggatt ggcggcagtt ggggcagttc gcggccggac aggtcggtag
    17581 tcgtgaggcg aatctgctcg cgggtcacgt cgccgcgaat gaccgtttca gttccgccga
    17641 tgatcagggg aaagtcccgc ccctcgtggc ccgccgggtg gaggctatac ttatgcatgt
    17701 ggccgcaaat cagcaaatcc aactggccgc gattcaggat cggcaccagg tgatcctgca
    17761 gccacttgcg gcggatgaac ttgggatcgg gatcgttggc gggcgggata tgcagcagcc
    17821 cgatgcggaa cggggcgcgc tggaaggcgg ggtcctggat ttgccgggcc agccactccg
    17881 cttcgcgatg cagatagggg tcgaaatcca ccaggccgga atattcactg ctgccgtccg
    17941 ctttatcttc gccgccgtcc agcaccagaa acatcaccgg cccctcctgc agggtgtagt
    18001 agtaacggcc ggagtccgtg ggaaaatagt ttagcaactg gcgcgcgaat ttgccgcgcg
    18061 tatcatggtt gccgcgcaca tacaccagcg ggatgcgggt cgcgaacggc ggaacaatcg
    18121 ggtccaccaa acagcgatat aactgcgatt cgctctccac cgagttcacc gcgtcgccat
    18181 tgaagaacac caaacccacc cgggcccagt ccaccgccgc cagggaggct gccagcggct
    18241 cgaccttctc atgccgatcg ttcaagacca caaaagccgc cgccggtttg cgcgggtcaa
    18301 aggtggtaaa gccatactcc gggctggcga tggttgcgcc gtaagtgacc tggtagggtt
    18361 tgaactcgac gatctcgcgc gaaaccacgc ggtagcggta ggccgtgccc ggctccaacc
    18421 cggccagcgc cacgttgtgc gtggtgacgt cggcatctat caggccatga tgcgccggaa
    18481 aattggtctg ccactgtgtg gctgattcca gccggtattc cacccgggaa acacccttgc
    18541 ggcttgtggc ccaggaaatg gtgatgcccg tcggcgaggg cgcttgcaga aacggcccat
    18601 gcgtgatggc gaaggcggcg ggcgcattac tcccggccgc cgccggtggc gggttttgcg
    18661 cgcgggccgc cgatgcggcg gcaagcgccc atccgacggc gagcgcgccg gcgccgaaga
    18721 gtccggcgcg ggccgccgcc gccgcgcgcc gccgcgcgtg ggcagacgag gtgcgggtca
    18781 acacgggaag aagagctgcc agtaatttaa tcatggatgt cctgatggta cccttgcagt
    18841 gatttgacgc cggccctgcc ggtgcgcgcg gctgcgatgc cggccaccgc ggctttggcg
    18901 gcggcggtcg tcgtcacgta agggattttg tacttgatgg cggccttgcg aatggcggcg
    18961 ccgtccgccg ccccggtctt gccgcgcggg gtgttgatga ccaggtcaat ctgcttgctt
    19021 ttgatctcat cggcaatatt gggccgatag tgctcgctga tcttgaagct gatctcgcag
    19081 gggatgccgt tggcctgcag aaacttgaag gtgctgtggg tggctttgag gcggaacccc
    19141 agctcggcaa agcgccgggc ggttggcagc atcaccggct tgtccttgtc ggtgacggag
    19201 atgaagacgg tgcccttgag cggcagcggg ctgcccgcgg cctcctggga tttgaagtag
    19261 gccaggtcga acgtcggcgc catgccgagg acttcgcctg tggaacgcat ttccggcccc
    19321 agcaccgggt ccacctccgg gaacatattg aaggggagca ccacttcctt gacgccggtg
    19381 tggcggatgg ggcggtggcg cagatcgagg tcgcgcagcc gctggcccag catcaggcgc
    19441 gtggcgatgg cggccatcgg aatattgcaa accttcgaga ccagcggcac ggtccgcgac
    19501 gcgcgcgggt tggcctccag gacatagacc ttgtcctggt gcaccgcgta ttggatgttc
    19561 atcaccccgc acaccttgag ctcgcggctg atgcgcagcg tgtattcctc gatggtcttg
    19621 atatgtttgc ggggcagggt caccggggga atggcgcagg cggaatcccc ggaatgaatg
    19681 cccgccagct cgatgtgctc catgatggcc gggataaacg cgtcctggcc gtcggccagg
    19741 gcgtcagcct cggcctcgac ggcattatcc agaaaccgat caatcaggat gggccgctcg
    19801 ggcgtgacct ccaccgccgc gtggatataa tgccggagca tttcctcatc gtgcacgatt
    19861 tccatgccgc gcccgcccag cacgaacgag ggccggacca tgaccgggta gccgatctgt
    19921 tcggcaatgc ggcgggcttc cgcgaagctg ctggccatgc ccgacgtggg catcgggatg
    19981 tccagttgct ccatcatctt ccggaaccgg tcgcggtcct ccgccaggtc aatcatgtcg
    20041 ggggtggtgc cgagaatcct gacgccggcg gcggccaggt cattggccag gttcaggggc
    20101 gtctggccgc caaactggac gacgacccct tccggcttct ccttttcgta aatggcaagc
    20161 acgtcctcca gggtcaacgg ctcgaagtag agcttgtccg aggtgtcata gtccgtcgaa
    20221 accgtttccg ggttgcagtt gaccatgatc gtctcgtagc cggcgtcccg cagcgtgaag
    20281 gcggcatgca cgcagcagta atcgaactcg atgccctggc cgatgcggtt gggaccgccg
    20341 cccagcacca tgaccttctt ccgaggcgtg gtcgccacgc ggtccggcgc gttgtaggtc
    20401 gagtagtagt aggcggcctt ctcgactccg ctcaccggca gggcgtccca agcctccgtc
    20461 accccgagtt tcttgcgctg ttcgcggatg cgcgcctcgg gaagcccgag cagcttggcc
    20521 aggtagcgat cggcgaagcc gtctttcttg gcctggatca ggagcgagtc gggcgggagc
    20581 ttgccctggt gcttgagaat ctgctcctcc aggtccacca gctccttcat ctgctggatg
    20641 aaccacggct tgatgtgggt cttgcgataa agctcgtcca ccgccgcgcc cttgcgcaac
    20701 gcctcgtaca tgatccactg gcgctcgctg ctcggctcgt tgagcagcag catgagctcc
    20761 tcgaggggct tgcgatgata atcccgcgcg aagcccaggc cgtagcggcc gttttccagg
    20821 gaacgaatcg ccttttggaa agcttccttg taggtcttgc cgatgctcat cacctcgccg
    20881 accgagcgca tctgcgtgcc cagcttgtcc tcggagcctt tgaatttctc gaatgcccag
    20941 cgcgggaatt tcaccaccac gtagtcgccc gagggcgtgt atttctccag cgtgccgtcg
    21001 cgccagtagg ggatttcgtc cagcgtgagg ccggcggcca gcttggccga gatcagcgca
    21061 atgggcaggc ccgtggcctt ggacgccagc gccgaagacc gcgacgtgcg cggattgatc
    21121 tcgatgatca ccacgcgccc ggtccgggga tcgtgcgcga attggacgtt ggtgcccccg
    21181 atcacgccaa tcgcctcgac gatccggtag gccagctcct gcagccgggc ctgcagccgg
    21241 ggatcaatcg tcagcatcgg cgccacgcaa taggaatccc ccgtatgcac gcccatcgca
    21301 tccacgttct cgatgaagca ggccgtgatc atctgatttt tggcgtcgcg caccacctcc
    21361 agctccagct cctcccagcc caccacggat tcctccacca ggatctgccc gacgatgctg
    21421 gcatccagcc cgcggctggc caccaaacgc agctcctcca cgttatagac atggccgccg
    21481 ccggtgccgc ccatggtgta ggccgggcga atgaccagcg ggtagcccag ctccgccgcc
    21541 acccgctccg cctcctccac gctgaacgcc ggcgcgctcc gcggcatttc gatccccagc
    21601 ttcttcatcg tctccttgaa gatgatgcga tcctcgccct tctcgatcgc gtcggcttcc
    21661 acgccgatca cgcgcacgcc atacttggcc agcacgcccg ctttggcgag ttgggacgaa
    21721 agattcaacc ccgtctgccc tcccaggttg ggcagcaagg cgtccggccg ctccttttcg
    21781 atgatctccg tcaacgccgc cacggtgagc ggctcgatat acgtcgcgtc cgccatgccc
    21841 ggatccgtca taatggtcgc gggattggag ttcaccagca cgacctgaaa acccaggctg
    21901 cgcagggctt tgcacgcctg ggtgccggaa taatcaaatt cgcacgcctg gccgatcacg
    21961 atcgggccgg agccaatgat aagcactttc ctgatgtcgg ttcgtcgcgg cataaaggtc
    22021 gttctgtcca gcggaacggc tggattgctt tccagaaccc gcccactacg gcgcgaagta
    22081 ttactgcgcc cccgcgccaa gtcaaacgcc gacgccaacg aacccacccg ctcatctgcg
    22141 aaacccgccg ccgccgaggc gatgcgccgt atcctgattg acaacgcccg ccggaagatg
    22201 cactgcaagg gcaacccgtc ggcgcgcggg cctctggccc gctttcagcg gtctattaac
    22261 aaaccgggtt ggatttgccc ttcatttagc gcgggcaatt gacaacgccc gccggaagat
    22321 gcactgcaag ggcaacccgt cggcgcgcgg gcctctggcc cgctttcagc ggtctattaa
    22381 caaaccgggt tggatttgcc cttcatttag cgcgggcaat tgacaacgcc cgccggaaga
    22441 tgcactgcaa gggcaacccg tcggcgcgcg ggcctctggc ccgctttcag cggtctatta
    22501 acaaaccggg ttggatttgc ccttcattca gcgcgggcaa tcctgaatat ttttgcatcc
    22561 gggcagattt aggggttgtg gggaaggaca attctccggg ttacagactg tagttccgct
    22621 gctgattgtt gagcattgaa agcgggccgg aggcccgcgc gccggggcgg aaatgacaca
    22681 ttttgggtaa tctttgactt ccagcgcgaa gtttgaggaa acgttaacgt taaaggtact
    22741 ctaaactgga gcggtttagt tgtgaaatac aatggtttgt cgtcatttca ccggaatctg
    22801 gggaggcata gccgggtagt gtcctaaaag ttctgcaaaa agcagcgggt catggtaagc
    22861 cactg
//
LOCUS       DAOPQG010000059        21952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_2972_length_21952_cov_14.692743, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000059 DAOPQG010000000
VERSION     DAOPQG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 21952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21952
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2972_length_21952_cov_14.692743"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1311
                     /locus_tag="PKI07_08220"
     CDS             100..1311
                     /locus_tag="PKI07_08220"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC51076.1"
                     /translation="MKVGACVVIGAVGLGLAGLVSCGQPDRGKPADPAAMPARPVSVA
                     RAQTRAIARTIGATGSLAAQEQATLSAKVAGRLERLEVDIGSELRQGDLVAQIEPRDY
                     ELGLQEAAAALAQARAALGLPIAGDDDRIELEQVTAVRQARAVLEEAAKNRERVQRLS
                     GSGIASQSEVDTVEATHTVAAARYDSALEEARGRMATVAQRRAEFDLARKRLADASVR
                     APFDGAVQARPATIGEYVAVGVPIVRLVKTARLRLRLDVPERECARVRVGQVVQLHVE
                     GDSRLYTGQIARLSPALDEQTRTLRVEADVPRQGSLRPGLFARAQIVVAEADPALTVP
                     EKAVITFAGLEKVVVVEDGRAVEKVVSTGHRGPDWVEIVSGLSAGETVVLNPGGLRTG
                     QRVARPEAEPN"
     gene            1314..4496
                     /locus_tag="PKI07_08225"
     CDS             1314..4496
                     /locus_tag="PKI07_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC51077.1"
                     /translation="MQKLAEICIRRPVFATMIVLSLVVVGMAGYLHLEVDRFPSVDLP
                     TVRVSTRLPGAAPAEVESQIAQPMEEVLNTIEGIQELRSICGSGAAFAVVTFDLKRDI
                     DAAAQDVRDRVATVVRDLPRDADPPTIAKAEPDQAPIMSIALAGNRSQRELSEIADKI
                     VKKQIERSAGVGQVELVGDLSRAINVWVDADRLAAYQVPITAVRDAVAAQNANIPGGN
                     VTGSLREETLRTMGRLPDARAFNDLVITTRQGTPIRVRDIGWAEDGTKEQRSISRLNG
                     EPTVALNVLRQSGANTVAVIEGVKQKLREVAPQLPADVKLQVTRDQSHYIYEALHEIQ
                     RHLVLGSLLACLVVLLFMRNWRAMIIAAVAIPTSVISAFGLMKALGFTLNSVTMLALV
                     LMVGIVIDDAIVVLENIFRFVEEKQMRPFAAARAATAEIGLAVMATTFSLVVIFVPVS
                     FMSSISGRFLYQFGFTAAVAVLVSLLVSFTLTPMMSARLLHMGNGRGRPDAGAGARSR
                     GGFYRRLDAGYLKLLRGALGRRWAVAGLAALVIASAAPLYQLVRQEYLPSNVDEGEFD
                     VRVTAPEGTSLTAMNEVSLRIEEELRSVPGVRLVLATAGSGFLGGVNGANFFVQLAPH
                     RERTFRWARLAHWPPWDAWRGNYTQRDIQQEIRRRLRQLPDLRMAIRNPQTFVGGGPN
                     SDIDFSLLGPDLDLLNTYAEHLRTNAPALGLLDADTTLKLDKPELRVEIDRERAANLG
                     VSTTDIANALRVMVGGDQRVTRFRDDTMSEDYDVQVRLKEGDRNEADPIRRLYVPRQN
                     GGLVRLDNVVKLVSTQAPSRIDRLDRQRQVSLRAAVAPGYAQADRIAALRAEVAKMGL
                     PTGYSTRVSGRARELETTFREFLFAFTLSVVLMYIILASQFESLVHPFTILLSLPLSV
                     PFAFLSLLAAGQTINLYSALGLLVLFGVVKKNAILQIDHMNKLRAAGLERGAAILQGN
                     RERLRPILMTTLTLIAGMLPLALGTGPGAEERRAVAVVVIGGQALCLLLTLVVTPVVY
                     SLLDDLRLWVRARWHRHTRAAGPEQLPRAA"
     gene            complement(4493..5854)
                     /locus_tag="PKI07_08230"
     CDS             complement(4493..5854)
                     /locus_tag="PKI07_08230"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC51078.1"
                     /translation="MNQRINRRQFLGATAVAGAGISLPWPVPAQPQKTGTGAADKPAL
                     LGGKPVRTRPPRAWPIFDEREEQALQAVVRSGKWFRGNGPHVARFEAAYAEATGARYC
                     LATNSGTSALFTSLAAVGVAAGDEVIVAPYTFIASVNAILLHHALPIFVDTDPETLQL
                     DARKIEAAITDRTTAILPVHLGGYPADLDTILAVAKPRRLPVIEDACQAHLGQWRQRC
                     LGTWGDTGCFSFQASKNLNCGDGGAILTSNEEWADQCYAFHNQCRPRRGGGGETAPGS
                     LRGGNFRMTEFQAAILLAQLTRLEEQMRIREQNAACLDRLLRDIPGIVPAKTYAGGTR
                     NAYHLFMFRYQREHFANLPRKRFLEALKAEGVEARAGYGRLNRAAFLQRALASRSWQR
                     LFPADTLAKWAERTECPANDRLAEEAAWFTQTTLLGTRADMEQIADALRKIQKHAAEL
                     ARG"
     gene            5891..6772
                     /locus_tag="PKI07_08235"
     CDS             5891..6772
                     /locus_tag="PKI07_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOC51079.1"
                     /translation="MQIESLKVFCDLTETKSFTRAAHINNVTQSAVSQTVSALERRFK
                     TPLIERSKRNFRLTPEGDVFYEGCKRILMTCDALRSRLQEIGQVVVGHLRVATTYSIG
                     LHVLPPHVKRFMLRHPGVQVHVEYRRNPQVYENVLGNIVDLGLVSYPVRDARLRVVPF
                     RKDTLVLICHPQHPLAGQKSVRVKALRGVKFINFAKGLPTRLALDALFKRQRVAVDSV
                     MQFDNIETIKRAVEINSGVAVVPRETVAKEVAIGSLVSVALEDGAYRRPLAVIYKRDR
                     VLSPAIKEFIAHLKQPV"
     gene            complement(6805..7764)
                     /locus_tag="PKI07_08240"
     CDS             complement(6805..7764)
                     /locus_tag="PKI07_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51080.1"
                     /translation="MTKTFVALNDPVHNLIRTLTRQLPLGPGESPDPRLEVRYHFTAS
                     DLPPAYYALAVFHSLGGITLESLQRTKWEVRECDRPAVPERVNYFDAVKLDRRSARDL
                     RLKVKRHGPGLLAADLLSFGVGALRARNDDPVELIHQYLWRHFGQLRSRHEVVYARIG
                     ALHWNSLPLLYERQKESFDSTNLVYGDALGELEQLWERRADTAFLERVVRVLQRMRAQ
                     AEIAAFLHPPLNLREIMRLHLRCQSVWKNPPATARALLRRVEYLLGNLVLESAPETVF
                     HGRAPAPPAPPRSRGGAPRRWRHDRSGYDGWLAGNSGPAGGPE"
     gene            7844..8941
                     /locus_tag="PKI07_08245"
     CDS             7844..8941
                     /locus_tag="PKI07_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HOC51081.1"
                     /translation="MPAITVSVNRPSPLAPWLPAAGALLLAALFSGCSTLPRPGAFAP
                     RKGDEIMVAGHWVHTGTRVVTWMDPGGYDAYRVERRFSPLEKSDWETSRQEVKYLDSP
                     NRYNLRRQGLSDEEIERVRGGGWDLPLLQRVVDQFVIHYDVCGTSRQCFKVLQDMRNL
                     SVHFMLDLDGTIYQTLDVKERAWHAGTANSRSVGIEIANMGAYSPQGRNPFAQWYAKD
                     ARGRTRITLPPAYGDGGLRTPGFVGRPARPEPVSGAIQGTELIQYDFTPEQYRALIKL
                     TAALCSALPKIKCDYPRDAAGHLVRQRLSDEELEAYQGVLGHYHIQANKVDPGPAFQW
                     DYVLGQARRLLKSGPPPASASLDQAPIRRRF"
     gene            9104..10537
                     /locus_tag="PKI07_08250"
     CDS             9104..10537
                     /locus_tag="PKI07_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOC51082.1"
                     /translation="MNLRIGIPGMALAAALAGTLLAAESPTWKAGTARANITPTQPLW
                     MAGYASRTNPATGKEMDLWIKALALEDARGHRAVILSGDVLGFTRTLHQRTCAALKQR
                     FALEPDQVLLAASHTHSGPVLAGALYDIYPLDAAQKDLIEKYTADLEARIVQTASAAL
                     TALAPARLTAGQGVAGFAVNRRNNPEPEVPRRLADGTLQGPVDHAVPVLAVYAPDGGL
                     KAVLFGYACHNTVMDYYRWSGDYAGFAQRALERSHPEATALFFIGCGGDQNPLPRHQL
                     EQAERYGLMLAAAVEETLRTPAPTLSPRLETALEMVELNLGPAPTEAELEKLAADSTP
                     YQRRWATRLLADLKAGRPFIRTYPFPVQAWRLGGQQALIALGGEPVVDWALLCKQQFG
                     PRTWVAGYCNDVMNYLPSQRVLQEDKPPLAQPRWGYEGALATIVYGLPAWRWADDVEE
                     RVTAGIRHVMAWPPGVSRPHRAEPQTP"
     gene            10934..11056
                     /locus_tag="PKI07_08255"
     CDS             10934..11056
                     /locus_tag="PKI07_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51083.1"
                     /translation="MSIPCTPLVHGLLGAGQDGEGGIVNFIHFSDLLDFERGRG"
     gene            11392..12138
                     /locus_tag="PKI07_08260"
     CDS             11392..12138
                     /locus_tag="PKI07_08260"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019526957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOC51084.1"
                     /translation="MYNKPDRKAWLKAGTAHCEPPPVAQHPPRRLVLLGAPGVGKGTQ
                     AELLCARLGACHLSTGDIFRAAKAMDPAERTPALTAALDYMRRGDLVPDETVLALVAE
                     RVGCLRCESGFLLDGFPRTVAQAEALERLLAGEQLRLDAVLSYELPLEKVVARLSGRR
                     TCAQCKAVFHVESRPPRVAGVCDHCGGKLYQREDDRPESIRVRMEAYERSTAPLADFY
                     RRQNLLVSISAEGTPEEICRRSLEALHAAA"
     gene            12125..12925
                     /locus_tag="PKI07_08265"
     CDS             12125..12925
                     /locus_tag="PKI07_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOC51085.1"
                     /translation="MRPRKWFFAGLAALALAGAGCAHTPGRGVKDSLPPPPAGTKWEL
                     AWADEFEGAVLDETKWNRLGDWPRRDGYWVREDACLSGRGTLLLRTRKDGERYTCGAV
                     NTRGKFEHAFGYYVARCKLPRQEGHWPAFWLMADGVNTVGNQGRDGTEIDIMEAPWRD
                     GRLTCNLHWDGYGKDHRSAGTNFCRPEVMEGWHTFSLLWLPEEYVFYVDGREIWRTAQ
                     GGVSQVPEFIKLTEEIGKWGGEIQKAALPDYFEVDYVRVYAARPRRLK"
     gene            13068..13143
                     /locus_tag="PKI07_08270"
     tRNA            13068..13143
                     /locus_tag="PKI07_08270"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13101..13103,aa:Lys,seq:ctt)
     gene            complement(13376..14635)
                     /gene="fabF"
                     /locus_tag="PKI07_08275"
     CDS             complement(13376..14635)
                     /gene="fabF"
                     /locus_tag="PKI07_08275"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOC51086.1"
                     /translation="MGSNWTERRVVVTGLGAVTPLGNQVDEFWQNLLAGRCGIGRITR
                     FDPARFDSQIAGEVKAFDPSPALPSPKEIRRTDRFSQFGLHAAWQALRDSGLDLERVN
                     RDEVGVLIGSGIGGLQTVSEQCQILSERGPGRLSAFMIPMLIVNMPSGIFSMYHKLRG
                     PNFATCSACATANHALGEAWRTVKMGDAQIMFAGGAEATIVPIGIGGFCAMKALSTRN
                     DDPARASRPFDRERDGFVMGEGAGVLVLEELEHAKKRGARIYAEIAGYGNTADANHVT
                     APSPDGEGAARCMKIAVRGGGLNLEAISYINAHGTSTPQGDIAETRAIKALFGDHARR
                     LAVSSIKGATGHMLGAAGAVEMIACVKAIQTGIIPPTINYEVPDPECDLDYVPNTPRS
                     MKIEAALNNSFGFGGHNASILVKKFNG"
     gene            complement(14702..14947)
                     /gene="acpP"
                     /locus_tag="PKI07_08280"
     CDS             complement(14702..14947)
                     /gene="acpP"
                     /locus_tag="PKI07_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOC51087.1"
                     /translation="MADKTIEQRVKDIIVEQLGVNADQVTPEAKFIEDLGADSLDTVE
                     LVMALEEEFGSEIPDEQAEKLQSVGDVIKYIEDLEQK"
     gene            complement(15024..15767)
                     /gene="fabG"
                     /locus_tag="PKI07_08285"
     CDS             complement(15024..15767)
                     /gene="fabG"
                     /locus_tag="PKI07_08285"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOC51088.1"
                     /translation="MNELAQQVAVVTGAGRGIGQAIALQLAAAGADIAGVDLQPESCA
                     ETVAKVQALGRRAWAFGANVADAASVTAAAEQILAAAGKVDILVNNAGITRDGLLMRM
                     SEADWDAVLDVNLKGTFLFTKAFTRSFLKQQSGRIINIASVIGLIGNAGQCNYGASKA
                     GVIGFTKSAARELASRGITVNAIAPGFIETAMTAKISPEARAALLKQIPLGSLGQPAD
                     VAGAVLFLAGPAGRYLTGQVLSVDGGMAM"
     gene            complement(15812..16753)
                     /gene="fabD"
                     /locus_tag="PKI07_08290"
     CDS             complement(15812..16753)
                     /gene="fabD"
                     /locus_tag="PKI07_08290"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOC51089.1"
                     /translation="MIKTALLFAGQGAQVVGMGRDWARELPTARSWFDRANAALGYDL
                     AALCFDGPEAELTRTENAQPGIFLVSWVALQLLRERVPGLSFQAAAGLSLGEFTALTA
                     AGALGFEDGLNLVRQRGRFMQEACAATRGGMAAIIGLDETPTREVCAQAGVTLANLNC
                     PGQLVISGPAEHIARACELARARGARRALPLPVAGAYHSPLMASAQPKLQAELERVTL
                     RPPAVPVIANVTARPHDGPETLRARLVEQVTSPVRWEESMRYLIEQGFTRFLELGPGT
                     ALTGFMKRIAKTAQVYNVADMASLAATAAALGADGAR"
     gene            16897..17130
                     /locus_tag="PKI07_08295"
     CDS             16897..17130
                     /locus_tag="PKI07_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51090.1"
                     /translation="MNQSGDRLRFLAQRSVHCVVCQRARRQQAGLAFWLVRTFARLCP
                     FCRAYAVVYGRKSYEPEPARPAPTGARERAPLV"
     gene            17146..18546
                     /locus_tag="PKI07_08300"
     CDS             17146..18546
                     /locus_tag="PKI07_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51091.1"
                     /translation="MNRILSAHTLFPLTAALLGAGVLISAAQDAAPRLVVPDGKAYKG
                     RKIIARVYDSHFALGHDSYNSMGTGSDGKIYYVLSSENIDQGAKMFCFDPKTQQIKEV
                     GDVTEACGEKGSKAIPQGKSHVNFVEKDHKLYFATHVGVYSYVDGKETMGIPPAGYKP
                     YPGGHLLSYDLKTGKFEDYGICPEREGVLTMNMDTRRGRLFALTWPSGIFYRYDLATR
                     NQKSFGKMCAEGENGTGPAYRTVCRSIVVDPRDGSAYFTSSEGTIFRYDAASDSVSAM
                     KGETMVKDYFGSYDPTDAGHMGYNWRQTFWREQDNLIYGVHGNSGYLFRFDPRVPRLE
                     VMQRITSEPSKRSGMFDQFSYGYLAFALGPDGKTIHYLTGAPIYIDGQRLAGKAKTAM
                     GEAKGLEDLHLITYDLKTGRYTDHGAVFYPDGQRPLYVNAMAIGKDGMIYTLPRITAN
                     GKTRSDLIAFPGPLKK"
     gene            complement(18665..19372)
                     /locus_tag="PKI07_08305"
     CDS             complement(18665..19372)
                     /locus_tag="PKI07_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOC51092.1"
                     /translation="MKTQAGLERALSFINCQLQPGGAGQTARETDAPRRAVTISRQTG
                     SGAHIVAQNLAAYLHAHEPKDACPWAVFDRNLVEKVLEDHNLPQRLARFMPEDRASEI
                     TDIMNELFDLHPPSWTLVHKTTDTILRLAELGNVILIGRGATVITAKLPYVFRVRIIG
                     SLEKRIKHVQELDHFTRPVARARILQEDRGRKRYLRKYFDADPDDPLLYHLIINTDDV
                     PYEEAARIIGEAVLRRR"
     gene            19843..21171
                     /locus_tag="PKI07_08310"
     CDS             19843..21171
                     /locus_tag="PKI07_08310"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC51093.1"
                     /translation="MSHSVLLFFRKRAVSLSLLGGLLGVLAPRPSLFAASVTLNWDAS
                     PSPDIVQYRVYYGVASAAYTHSVPAGTNLTVSISNLAGGLTYYFAATATDINGTESDY
                     SAEIAALIPLNQANRPPTLNALANLTLNENAGPQTVNLLGISSGAANESQTLTVTASS
                     SNPGLIPTPTVNYTSPNASGTLSFTPVAYAYGSATITVTVNDGATVNNLISRSFTVTV
                     NSVNQPPTLNALPNLTLNQGAGRQTVNLSGITAGAPNESQTLTVTASSSNPGLIPTPA
                     VNYTSPNTTGSLSFTPVATASGAATITVIVNDGGASNNLIARAFTVTVNPANQPPTIS
                     ALADYVMAANTTTPPIPFTISDPETDAASLTLAGNSDNPGLVPVSGIVFGGSGSHRTV
                     TLTPRPGQIGSAHITLTVSDGANTFSRVFLLTVRLRPEAPVGFHTVSAGF"
BASE COUNT         3510 a         7539 c         7276 g         3627 t
ORIGIN      
        1 ccagcggctc cgccacgcaa aagcccgggc gcgcctgaaa ttggctttgg gcgttgattt
       61 ctttcgccat aaggccgggt agcggcatag tatgagaaca tgaaagtcgg tgcttgcgta
      121 gtcatcggag cggtgggatt gggcctggcg ggcctggttt cctgcggcca acccgatcgt
      181 gggaagccgg cggaccccgc agccatgccc gcgcgtccgg tgagcgtcgc gcgcgcgcaa
      241 acgcgggcga tcgcccggac tatcggggcg accgggtcgc tggcggcgca agagcaggcc
      301 accctcagcg ccaaagtggc ggggcgcctg gagcgcctgg aggtggatat cggcagcgag
      361 ttgcgccagg gcgacctcgt cgcgcagatc gagccgcgtg attacgagct gggattgcag
      421 gaagcggcgg cggccctggc gcaggcgcgc gcggcgctgg ggcttccgat cgccggcgac
      481 gatgaccgga tcgagttgga acaggtgacc gctgtgcggc aggcccgggc ggtgctggag
      541 gaagcggcga agaaccggga gcgcgtccag cggctgtcag gctcgggcat cgcgtcccaa
      601 tcggaagtgg acaccgtgga agccacccac acggtggccg ccgcgcgcta cgactcggcc
      661 ctggaagaag cccgcggccg gatggcgacg gtcgcgcagc ggcgggcgga gtttgacctg
      721 gcccgcaaac gcctggccga tgcctcggtg cgcgcgccct ttgacggcgc ggtgcaggcg
      781 cggccggcca cgatcggcga atatgtcgcg gtgggggtgc cgattgtgcg cctggtaaaa
      841 accgcccggc tgcggctgcg cctggacgtg cccgagcgcg agtgcgcccg ggtgcgcgtt
      901 ggccaggtgg tgcaactgca cgtggagggt gattcccggc tttacaccgg gcagattgcc
      961 cggctcagcc cggcgctgga tgagcaaacc cgcacgctgc gcgtggaagc cgacgtgccc
     1021 cggcaggggt cgctgcggcc cgggctgttc gcgcgggcgc agatcgtcgt ggccgaggcc
     1081 gaccccgcgc tcacggtgcc cgagaaggcc gtgatcacgt tcgccgggct ggagaaggtc
     1141 gtcgtggttg aggacggccg ggcggtggag aaagtcgtga gcaccgggca ccgcgggccg
     1201 gactgggtgg agattgtctc cggtttgtca gccggggaaa cggtcgtgtt gaaccccggc
     1261 ggcctgcgca cgggccagcg ggtggcccgc ccggaggcgg agccgaactg acgatgcaga
     1321 aactggctga gatttgcatc cggcggccgg tcttcgccac gatgatcgtg ctgagcctgg
     1381 tggtggtggg gatggcgggc tacctgcacc tcgaggtgga tcgctttccc tcggtggacc
     1441 tgcccacggt ccgggtcagc acgcgcctgc cgggcgccgc gccggcggag gtggagtccc
     1501 agatcgccca gcccatggaa gaagtcctca acaccatcga gggcatccag gaactgcgct
     1561 cgatttgcgg ctccggcgcc gcgtttgccg tcgtcacctt cgacctgaag cgggatattg
     1621 acgcggcggc gcaagacgtg cgcgaccggg tggcgacggt ggtgcgcgat ctgccccgcg
     1681 acgccgaccc gcccacgatc gccaaggcgg agccggacca ggctcccatc atgagcattg
     1741 cgctggcggg caaccggtcg cagcgcgagt tgtccgagat cgcggacaag attgtgaaaa
     1801 agcagatcga acgctccgcc ggcgtggggc aggtggagct ggtcggcgat ttgtcgcggg
     1861 cgatcaacgt gtgggtggac gccgaccggc tcgccgccta ccaggtgccg attaccgccg
     1921 tgcgggacgc ggtcgccgcc caaaacgcga acatcccggg cggcaatgtc acgggcagcc
     1981 tgcgggagga gacgctgcgg acgatggggc ggctgcccga cgcccgcgcg ttcaacgacc
     2041 tggtgattac cacgcgccag ggcacgccca ttcgcgtgcg ggacatcggc tgggccgagg
     2101 acggcacgaa ggaacagcgt tccatttccc ggctcaacgg ggagcccacc gtcgcgctca
     2161 acgtgctgcg ccagtccggc gccaataccg tcgccgtcat cgagggcgtc aagcagaagc
     2221 tgcgcgaggt ggcgccgcag cttccggccg acgtcaagct gcaggtcacc cgcgaccaat
     2281 cgcactacat ctacgaggcc ttgcacgaaa tccagcggca cctggtcctg ggcagcctcc
     2341 tggcctgcct ggtggtgctg ctgttcatgc gcaactggcg cgcgatgatc atcgccgcgg
     2401 tggccatccc cacctccgtg atttccgcct ttggcctgat gaaggcgctg gggttcaccc
     2461 tcaacagcgt caccatgctc gcgctggtgt tgatggtggg aatcgtcatt gatgatgcca
     2521 tcgtggtgct ggaaaacatc ttccggttcg tcgaggaaaa gcagatgcgc cccttcgcgg
     2581 cggcgcgcgc ggccacggcc gagatcgggc tggccgtcat ggccaccacg ttcagcctgg
     2641 tggtgatctt cgtcccggtt tccttcatgt cgagcatctc cgggcgcttt ctctaccagt
     2701 tcggcttcac ggcggcggtg gcggtgctgg tgagcctgct ggtctcgttc acgctgacgc
     2761 cgatgatgag cgcccgcctg ctgcacatgg gcaacgggcg cggccgcccg gacgccgggg
     2821 ccggggcgcg ctcccgcggc gggttttatc gccgcctgga tgccggctac ctgaaactcc
     2881 tgcgcggcgc cctggggcgc cggtgggcgg tggcggggct ggccgcgctg gtgatcgcct
     2941 ccgcggcgcc gctctaccag ttggtccggc aggaatacct gcccagcaac gtggatgagg
     3001 gcgaatttga tgtgcgcgtc accgcgcccg aaggaaccag cctgacggcg atgaacgagg
     3061 tgtccctgcg catcgaggag gaattgcgtt cggtgcccgg cgtgcgcctg gtgctggcca
     3121 ccgccggcag cgggttcctg ggcggcgtca acggcgccaa ctttttcgtc cagctcgcgc
     3181 cgcatcgcga acgcaccttc cgctgggccc ggctggcgca ctggcccccg tgggatgcct
     3241 ggcgcggaaa ttacacgcaa cgcgacatcc agcaggagat tcgccgccgc ctgcgccagt
     3301 tgccggacct ccggatggcc atccgcaacc cgcagacatt cgtcggcggc ggccccaact
     3361 cggacattga tttttcgctg ctcgggccgg acctcgattt gctgaacacc tacgccgagc
     3421 atctgcgcac caacgcgccg gcgctgggcc tgctggatgc cgacaccacg ctcaagctcg
     3481 acaagcccga gctgcgcgtg gaaattgacc gcgagcgcgc cgccaacctg ggcgtcagca
     3541 ccaccgacat cgccaacgcc ctgcgcgtca tggtgggcgg cgatcagcgc gtgacccgtt
     3601 tccgcgacga caccatgagc gaggattacg atgtgcaggt gcggttgaag gagggcgacc
     3661 gcaacgaagc cgaccccatc cgccggctgt acgtcccgcg gcagaacggc gggctggtgc
     3721 ggctggataa cgtggtgaag ctggtgtcca cccaggcgcc ctcgcgcatt gaccggctgg
     3781 accgccagcg gcaggtgagc ttgcgggcgg cggtggctcc cggctacgcg caggcagacc
     3841 gcatcgccgc cctgcgcgcc gaggtggcga agatggggct gccgaccggc tattccacgc
     3901 gcgtctcggg ccgcgcgcgc gaattggaaa ccaccttccg ggagttcctg ttcgccttca
     3961 ccctgtcggt cgtcctgatg tatatcatcc tggcctccca gttcgagagt ctcgtgcacc
     4021 cgttcaccat cctgctctcg ctgccgcttt cggtgccgtt tgccttcctt tccctgctgg
     4081 ccgccggcca gaccatcaac ctctattccg cgctcggcct gctggtcctc tttggcgtcg
     4141 tcaaaaagaa cgccatcctc cagatagacc acatgaacaa gctccgggcc gccgggctgg
     4201 agcgcggcgc ggcgatcctc cagggcaacc gcgagcggct gcgcccgatt ctcatgacca
     4261 cgctgacgct gatcgccggg atgctgccgc tggcgctggg caccgggccg ggggccgaag
     4321 aacgccgggc cgtggcggtg gtggtcatcg gggggcaggc gctgtgcctg cttctgacgc
     4381 tggtcgtgac gcccgtggtc tattcgctcc tggatgattt gcggctgtgg gttcgtgcgc
     4441 gctggcatcg gcatacccgc gccgccgggc cggagcagct tcctcgcgcc gcttaacccc
     4501 gggccagctc cgcggcgtgc ttctgaatct tgcggagggc gtcggcgatc tgctccatgt
     4561 ccgcgcgcgt gcccagcagg gtggtttgcg tgaaccacgc ggcttcctcg gcaagccggt
     4621 cattggccgg gcattcggtg cgctccgccc acttggccag cgtgtccgcc ggaaacaggc
     4681 gctgccagga gcgggacgcc agcgcccgct ggaggaacgc cgcccggttc agccggccgt
     4741 agcccgcgcg ggcctccacg ccctcggcct tgagggcttc caggaagcgc ttccgcggca
     4801 ggttggcaaa gtgctcccgc tggtagcgga acatgaacag gtgataagcg ttgcgcgtgc
     4861 cgccggcgta ggttttcgcc ggcacaatcc cggggatgtc ccgcagcagg cggtccaggc
     4921 aggcggcgtt ctgctcgcgg atccgcattt gctcttccag ccgggtcaac tgggccaaca
     4981 ggatggcggc ctgaaactcg gtcatgcgaa aattgccgcc ccgcaggctg cccggggccg
     5041 tttcgccgcc gccgccgcgc cggggccggc actggttgtg gaacgcatag cactggtcgg
     5101 cccattcctc attgctggtg agaatcgcgc cgccatcgcc gcagttcagg ttcttgctgg
     5161 cctgaaaact gaagcagccg gtgtcgcccc acgtgcccag gcaccgctgc cgccactgcc
     5221 ccaggtgcgc ctggcaggcg tcctcgatca ccggcagccg gcgcggcttt gccacggcca
     5281 ggatcgtatc caggtcggcg ggatagcccc ccaggtgcac ggggaggatg gctgtggtgc
     5341 ggtcggtgat cgcggcttca attttgcgcg cgtccaactg cagggtttcc gggtcggtat
     5401 ccacgaaaat gggcagggca tggtgcagca ggatggcgtt gaccgaggca ataaaagtgt
     5461 agggcgcgac gatgacctcg tcacccgccg cgacgccgac cgccgccagg gaagtgaaga
     5521 gggcgctggt cccgctgttg gtggccaggc agtaccgggc ccccgtggcc tcggcgtaag
     5581 ccgcctcgaa acgcgccacg tgggggccgt tgccccggaa ccatttgccg ctgcgcacca
     5641 ccgcctgcag ggcctgctcc tcgcgctcgt caaagatcgg ccaggcgcgg ggcgggcgcg
     5701 ttcggaccgg cttgccgccg agcagggccg gcttatccgc cgcgcccgtg ccggtcttct
     5761 gaggttgagc cgggacgggc cagggaaggg agattcccgc gccggctacc gcggtggcgc
     5821 ccaggaactg ccgccggtta atacgctggt tcatgttgac ggattcctaa ttaggggatt
     5881 aaatggacta atgcaaatcg aaagtctcaa ggtgttctgc gacctgacgg aaaccaaaag
     5941 cttcacgcgc gccgcacaca tcaacaacgt gacccagtcc gccgtcagcc agacggtcag
     6001 cgccctggaa cgccgcttca aaacgccgct catcgagcgc agcaagcgga acttccggct
     6061 gacccccgag ggcgatgtct tttacgaggg ctgcaaacgc attctgatga cctgcgacgc
     6121 gctccggagc cggctgcagg agatcggcca ggtggtcgtc ggccacctgc gcgtggccac
     6181 cacttacagc atcgggctgc acgtgctgcc gccgcacgtg aaacgcttca tgctccggca
     6241 ccccggcgtc caggtgcacg tcgagtaccg ccgcaatccc caggtctatg agaacgtgct
     6301 gggcaacatc gtggacctgg ggctggtgtc ctaccccgtc cgcgacgcgc ggctgcgcgt
     6361 ggtgcctttc cggaaggata cgctggtgct gatctgccac ccgcagcatc ccctggccgg
     6421 gcagaaatcc gtgcgcgtca aagccctccg gggggtgaag ttcatcaact tcgccaaggg
     6481 cctgcccacg cgcctggcgc tggacgccct cttcaaacgc cagcgcgtgg cggtggactc
     6541 ggtcatgcag ttcgacaaca tcgagaccat caagcgggcg gtcgagatca attccggggt
     6601 ggccgtggtg ccgcgggaaa ccgtcgccaa agaggtcgcc atcgggagcc tggtctccgt
     6661 ggccctcgag gacggcgctt accgccgccc gctggcggtc atctacaagc gggaccgggt
     6721 gttgtcgccg gccatcaagg agtttatcgc ccacctgaag caaccggttt gaagccgcgg
     6781 cggcccggaa cgccgcgccc gcgcctactc cggcccgccg gcgggcccgc tgttgcccgc
     6841 cagccagcca tcgtaacccg accggtcatg ccgccagcgc cgcggggcgc cgccccggct
     6901 gcgcggcggc gcgggcgggg ccggcgcgcg cccgtgaaag accgtctccg gggcgctctc
     6961 cagcacgagg ttgccgagca ggtattccac gcgccgcaac agggcgcggg cggtggcggg
     7021 cgggtttttc cagacgctct ggcaccgcaa gtgcagccgc atgatttcgc gcaggttcag
     7081 cggcggatgc aggaacgccg cgatctcggc ctgcgcgcgc atgcgctgga gcacccgcac
     7141 cacgcgttcg agaaaagccg tgtcggcccg ccgctcccaa agctgctcca gctcgcccag
     7201 ggcatccccg taaaccaggt tcgtggagtc aaaggactcc ttctgccgct cgtacagcag
     7261 cggcagcgag ttccaatgca gcgccccgat gcgcgcatag acgacctcgt gccggctgcg
     7321 gagctgccca aagtgacgcc agagatactg gtggatcagc tcgacggggt cgtcgttgcg
     7381 cgcgcgcaaa gcccccaccc cgaagctgag caagtccgcc gccagcaggc ccggcccatg
     7441 ccgcttgacc ttcaaccgca ggtcgcgcgc gctgcgccgg tccagcttga cggcatcgaa
     7501 gtagttcacc cgctcgggaa ccgccggccg atcgcactcg cggacctccc actttgtccg
     7561 ctgcagcgat tccagcgtga tcccccccag ggaatgaaac acggccagcg cataatacgc
     7621 cggcggcaga tccgacgccg tgaagtggta gcgcacctcc aggcgcggat ccggcgactc
     7681 ccccggcccc aggggaagct ggcgcgtgag cgtccggatc aggttgtgaa ccgggtcatt
     7741 cagcgcgaca aaggtcttcg tcattcgccc tgaaaccagc tagaaacgta ccagtcgcac
     7801 gacataaact tgacgcgccg ggcgccgccg cgacaatacg cccatgcctg caataactgt
     7861 ttcggtgaat cgtccctccc ccctcgcccc ctggctcccg gccgctggcg ccctcctcct
     7921 ggccgcgctt ttttccggct gctccacgct gccccgcccg ggcgcctttg cgccccgcaa
     7981 gggagatgaa atcatggtcg ccggccactg ggtgcatacc ggcacgcggg tcgtgacctg
     8041 gatggacccc ggcggctacg acgcctaccg cgtcgaacgc cggttcagcc cccttgagaa
     8101 gtcagactgg gaaacctccc gccaggaggt caaatacctc gattccccca accgctacaa
     8161 cctgcgccgc caggggcttt ccgacgagga aatcgagcgg gtgcggggag gcggctggga
     8221 tttgccgctg ctgcagcggg tcgtggatca gttcgtcatc cactatgatg tctgcggcac
     8281 cagccgccaa tgcttcaagg tcctgcagga tatgcgcaac ctgagcgtgc atttcatgct
     8341 cgacctcgac ggcacgatct accagacgct cgatgtcaag gaacgcgcct ggcatgccgg
     8401 caccgccaac agccgctccg tcggcatcga aatcgccaac atgggcgcat actccccgca
     8461 gggccggaac ccgtttgccc aatggtacgc caaagacgcc cgcggccgga cgcgcatcac
     8521 cctccccccg gcctacggcg acggcggcct ccgcaccccg ggctttgtgg gccgccccgc
     8581 gcggcccgag ccggtgagcg gcgccatcca gggcacggaa ttgattcaat acgacttcac
     8641 gcccgagcaa taccgggcct tgatcaaact caccgccgcg ctttgctcgg cgctgcctaa
     8701 aatcaaatgc gactatcccc gcgatgccgc cggccacctc gtccgccaga gattgtccga
     8761 cgaggagctg gaggcctacc agggcgtcct cggccattat cacatccagg ccaacaaagt
     8821 ggaccccggc ccggccttcc aatgggatta cgtcctcggg caggcccgcc gcctgctcaa
     8881 gagcggcccg ccgcccgcct ccgcctctct cgaccaggct cccatccgcc ggcgattcta
     8941 acgcccgcgc tccgctccgg cccgcgggct ggcggccgcc cgcgcgctgc cgaaacggct
     9001 cggcatccgc gcgcctcccg ccgcgcgcgg cgggcccccg ggccaatccc cgccccgctt
     9061 tttccgcttg cccggcaggg gcgcgaagag aagattaagc attgtgaact tgcgcatcgg
     9121 tattcctgga atggcgctgg ccgcggcgct ggcgggcacc ctgttggcgg ctgaatcgcc
     9181 aacctggaaa gccggcacgg cccgcgccaa catcaccccc acgcagcccc tctggatggc
     9241 gggctatgcc tcccgcacca accccgccac gggcaaggaa atggatctct ggatcaaagc
     9301 cctggccctc gaagacgcgc ggggccaccg cgccgtcatc ctgagcggcg acgtgctggg
     9361 ctttacccgc accctccacc agcgcacctg cgcggccctc aagcaacgct tcgcgctgga
     9421 gccggaccag gtgcttctcg ccgcctcgca cacccatagc ggcccggtgc tggccggcgc
     9481 gctctacgac atctatcccc tggacgcggc ccaaaaggac ttgatcgaaa aatacaccgc
     9541 cgacctcgaa gcccggatcg tccagaccgc cagcgcggcc ctgacagccc tggcccccgc
     9601 ccgattgacg gccgggcagg gcgtcgccgg gtttgccgtc aaccggcgga acaacccgga
     9661 accggaggtg ccacggcgcc tcgcggacgg gaccttgcaa ggcccggtgg accacgcggt
     9721 gccggtgctg gcggtttatg cgccggacgg cggcctcaaa gccgtgctct tcggctacgc
     9781 ctgccacaat accgtcatgg actattatcg ctggtccggc gactacgccg gcttcgccca
     9841 gcgcgcgctc gaacgcagcc accccgaagc caccgccctg ttcttcatcg gctgcggcgg
     9901 cgaccaaaac cccctgccgc gccaccagct cgaacaggcg gaacgctacg gcctcatgct
     9961 cgccgccgcc gtcgaagaaa ccctgcggac acccgcgcca acgctgtccc cccggctcga
    10021 aaccgccctg gaaatggtgg aactcaacct cggccccgcg ccgaccgaag ccgagctgga
    10081 aaaactcgcg gcggactcaa ccccttatca gcgccgctgg gcaacccgcc tgctggccga
    10141 cctgaaagcg ggccgtccgt tcatccgcac ctatcccttc ccggtgcaag cctggcggct
    10201 gggcggccag caagccctca ttgcgctcgg cggcgagccg gtggtggatt gggcgctcct
    10261 gtgcaaacaa cagttcggcc cgcggacctg ggtggcgggc tattgcaacg acgtgatgaa
    10321 ctatctcccc tcacagcgcg tgctccagga ggataaaccg cccctggccc agccgcgctg
    10381 gggctacgaa ggcgccctgg ccaccattgt ctatggcctc cccgcctggc gctgggccga
    10441 cgacgtggaa gaacgggtca ccgcgggaat ccggcacgtc atggcctggc cgccgggcgt
    10501 gtcccgcccc cacagggcgg aaccccaaac cccttaacct taatccgcgc atttcgcgcc
    10561 cccggccgcc tatgggcagg cggttggccc acgcgcggca ggcagccgcc ctttccggtt
    10621 aacgctcaaa tagcgcgtcc atagcccccc agccgggaaa gagcgccttt ccgcgaaccc
    10681 caggcctcaa accgccggga aaccaaggct cgccaggccg tctgtccccc gatccgggcg
    10741 tttctccggc ctggccctcg ttttcggggc gatttggctg ttttgcccaa agccatcccc
    10801 ccaaccccgc tttcggcctc gctctgcctt tttccccgtc tcctcgttcc tccaccgctc
    10861 ccaataagcc gtgtacaagg ggtgtacaag ggatgcccac cgggagtcca tgggatttta
    10921 gctggtacac ccgatgagca tcccttgtac accccttgta cacggcctgc tgggagcggg
    10981 gcaggatggg gagggtggca ttgttaactt cattcatttc agcgacttgc tggatttcga
    11041 gcgggggcgg ggctgaaata agggcgtttt gggactggtt ttgggcgttt ttgccgttcc
    11101 gggcttgggt aagggcgatt cggggcgttc agatgggggt ggtttggaag gaggccaaag
    11161 accgaagggg ctttattggc aggaccgagg ttccgtccgg gtggcgcgga gggaatccaa
    11221 tagtttcaca agggaggcgg acttgccccg ggggcgggag tgcgggccgg cgggaaggtt
    11281 ggcgccgctt tcgaggcttt cagcggcggc ccggttttat gagagaaacg tccggtttaa
    11341 tctggccgcg ccggggaaac ctgttatacc tgtgttaatt cagtcgcaaa tatgtataac
    11401 aaaccagatc gaaaagcctg gctcaaagcc ggaaccgctc actgtgaacc gcctcccgtc
    11461 gcgcagcatc cgccgcggcg gctggtcttg ctgggcgcgc cgggggttgg caaggggacg
    11521 caggcggaat tgctgtgcgc ccggctgggg gcgtgccatt tgtccaccgg cgacatcttt
    11581 cgcgcggcca aggcgatgga tccggccgag cgcaccccgg cgctgaccgc ggccttggat
    11641 tacatgcggc ggggcgattt ggtgccggac gagacggtgc tggcgctggt ggcggagcgg
    11701 gtgggttgcc tgcgctgcga gagcgggttt ttgctggatg gctttccgcg cacggttgcc
    11761 caggccgagg cgctcgagcg gctcctggcc ggcgagcaac tgcggctcga cgcggtgctg
    11821 agctacgagc tgccgctgga gaaagtggtg gcgcggttga gcgggcggcg gacgtgcgcg
    11881 cagtgcaagg cggtgtttca tgtggaaagc cggccgcccc gggtggccgg ggtgtgcgat
    11941 cactgcggcg gcaagctgta tcagcgcgag gatgaccgcc ccgaatccat tcgcgtgcgc
    12001 atggaagcct acgagcgcag caccgcgccg ctggcggact tttaccggcg ccagaacctg
    12061 ctggtctcca tttccgcgga aggcacgccg gaggaaatct gccggcgctc cctggaggcg
    12121 ctccatgcgg ccgcgtaaat ggtttttcgc cgggctggcg gccctggccc tcgcgggcgc
    12181 cggctgcgcc cacactcccg ggcgcggcgt caaagacagc ctgccgccgc cgccggcggg
    12241 gacgaaatgg gagctggcct gggcggacga gttcgagggg gccgtgctgg atgaaaccaa
    12301 gtggaaccgg ctgggggact ggccgcggcg cgatggctac tgggtccggg aggatgcctg
    12361 cctgagcggc cggggcaccc tgctgctgcg cacgcgcaag gacggggagc gttacacctg
    12421 cggcgccgtc aacacccgcg gcaagttcga gcacgccttc ggctactacg tggcgcgctg
    12481 caagctgccc cggcaggaag gccattggcc cgcgttctgg ctgatggccg acggcgtgaa
    12541 caccgtgggc aaccagggcc gggacggcac ggagattgat attatggaag ccccctggcg
    12601 cgacgggcgc ctgacctgca acctgcactg ggacggctac ggcaaagacc atcggtcggc
    12661 gggaacaaac ttttgccgcc cggaagtgat ggagggctgg catacgttca gcctgctatg
    12721 gctgccggag gaatatgtgt tttacgtgga cgggcgggaa atctggcgca ccgcccaggg
    12781 cggcgtctcc caggtgccgg agttcatcaa gctcaccgag gaaatcggca agtggggcgg
    12841 cgagattcaa aaggccgcgt tgccggacta tttcgaggtg gattacgtcc gcgtgtatgc
    12901 ggcccggccc cggcggctga aatgagcggc ccggcgtccg cgcccgccgc cgttttccgg
    12961 ccgggcgcgc ccggcaaaag aaccgcaaaa gggcttgctt ttcccggcgg ttcatgcgaa
    13021 atacaacccc gcgctgatgc cgtgccgcca cggcgcgggc gccgggtgcc tcggtagctc
    13081 aattggtaga gcagttgact cttaatcaat tggttctagg ttcgagtcct agccggggta
    13141 ccaacctcct ccccgccggt ttcttcctcc gaaaagcctc gcaaccccgc gtcgcggaaa
    13201 ggtccgccgc cgccccgcag cccgcggacg ggaaggcggg gcgcggggag gcaaagcgcg
    13261 ggcccggggc gggaggaacg ggtttgcggg cgcgggcgcg cgggcgcgcg gggttcgcgg
    13321 gggcgggtgc ggtgggggcg gcggcggttc cgcccggggc gaggcccggc gcgggtcagc
    13381 cgttgaactt tttgaccagg atgctggcgt tgtggccgcc gaagccgaag gaattgttga
    13441 gggcggcctc gatcttcatg gagcgcgggg tgttgggcac gtaatccagg tcgcactcgg
    13501 gatccggcac ttcgtaattg atggtgggcg ggatgatgcc ggtctggatc gccttgacgc
    13561 aggcaatcat ttcgaccgcg cccgccgcgc ccagcatgtg gccggtggcg cccttgatgg
    13621 agctgacggc gaggcggcgc gcgtggtcgc caaagagggc cttgatggcc cgggtttcgg
    13681 cgatgtcgcc ctggggggtg gaggtgccgt gggcgttgat gtaggagatg gcctcgaggt
    13741 tgaggccgcc gccgcgcacg gcgattttca tgcagcgggc ggcgccctcg ccgtccgggc
    13801 tgggggcggt gacgtggttg gcgtcggcgg tgttgccgta gccggcgatt tcggcgtaga
    13861 tgcgggcgcc ccgttttttg gcgtgctcca gttcttcgag cacgagcacg cccgcgccct
    13921 cgcccatgac gaagccgtcg cgttcccggt caaacgggcg cgaggcgcgg gcggggtcgt
    13981 cgttgcgggt gctgagcgcc ttcatggcgc agaagccgcc gatgccgatg gggacgatgg
    14041 tggcctcggc gccgccggcg aacatgattt gcgcgtcgcc catcttgacg gtgcgccagg
    14101 cttcgcccag ggcgtggttg gcggtggcgc aggccgagca ggtggcgaag ttgggccccc
    14161 gcagcttgtg gtacatcgag aagatgcccg agggcatgtt gacgatgagc atggggatca
    14221 tgaaggccga gagccggccc gggccgcgct cgctcaggat ctggcattgt tccgagacgg
    14281 tctgcaaccc gccgatcccg gagccgatga gcacgcccac ttcgtcgcgg ttgacccgct
    14341 cgaggtccag gcccgagtcg cgcagggcct gccaggcggc gtgcaagcca aactgggaga
    14401 agcggtcggt gcgccggatt tccttggggg agggcagggc ggggctgggg tcgaatgcct
    14461 tcacctcgcc ggcgatttgc gaatcgaacc gggcggggtc gaagcgggtg atgcggccga
    14521 tgccgcaccg gccggccagc aggttctgcc aaaactcgtc cacctggttg cccagcgggg
    14581 tcacggcccc cagcccggtg acgacgacgc ggcgttcggt ccaattactg cccataaaaa
    14641 tcaaacgggg cagccctcgc tgatgccagt tcgagccacc ccggaactct tcacggtcga
    14701 attacttttg ctccaggtct tcgatgtatt tgatgacgtc gccgacgctc tgcagctttt
    14761 cggcctgctc gtcgggtatc tcgctgccga attcctcttc gagcgccatg accagttcca
    14821 ccgtatcgag ggaatccgcc ccgaggtctt cgatgaattt ggcctcgggc gtgacctggt
    14881 ccgcattcac ccccagttgc tcgacaatga tgtctttgac tcgttgttca atcgtcttat
    14941 ctgccatgtg tttctccagt tcttcgtttt gcagtgtgtc tatgttaagg ttcaaagggc
    15001 gtcaagcccg aagcgaaaat aaattacatg gccatgccgc cgtccaccga aagcacctgc
    15061 ccggtgaggt agcggccggc gggcccggcc aggaacagga cggcgccggc cacgtccgcg
    15121 ggctggccca ggctgcccag cgggatttgc ttcagcagcg ccgcgcgcgc ctcggggctg
    15181 atcttcgccg tcatggcggt ctcgatgaag ccgggggcga tggcgttgac ggtgatgccg
    15241 cgggacgcca gctcccgggc ggcggacttg gtgaagccga tgacgccggc tttgctcgcg
    15301 ccgtagttgc actgcccggc gttgccgatc aggccgatga cggaggcgat gttgatgatg
    15361 cggccggatt gctgcttgag gaagctgcgg gtgaaggcct tggtgaacag gaaggtgccc
    15421 ttgaggttga cgtccagcac cgcatcccag tcggcctcgc tcatgcgcat cagcaggccg
    15481 tcccgggtga tgccggcgtt gttgaccagg atgtccacct tgccggcggc ggcgaggatc
    15541 tgctccgcgg cggccgtcac gctggcggcg tcggcgacgt tggccccgaa ggcccaggcc
    15601 cggcggccca gcgcctggac tttcgccacg gtctcggcgc aggattccgg ttgcaggtcc
    15661 accccggcga tgtcggcgcc ggcggcggcc agttgcaggg cgatggcctg gccgatgccg
    15721 cgcccggccc cggtcaccac ggccacttgt tgggctaatt cgttcataaa acgcgcttct
    15781 tttccgctat ccgcgggcgc ttgtcaagcc gctaccgcgc cccgtccgcg cccagggccg
    15841 ccgccgtggc ggccaggctg gccatatccg ccacgttata cacctgcgcg gtcttggcga
    15901 tccgcttcat aaaacccgtc agcgcggtgc cggggcccag ttcgaggaag cgggtgaagc
    15961 cctgctcgat caggtagcgc atggactcct cccagcgcac cggcgaggtg acctgctcga
    16021 ccaggcgggc gcggagcgtc tccgggccgt cgtgcggccg ggcggtgacg ttggcgatga
    16081 ccgggacggc gggcgggcgc agggtcaccc gctccagctc ggcctggagt ttgggctggg
    16141 cgctggccat gaggggcgaa tgataggcgc cggcgacggg cagcggcagc gcgcggcggg
    16201 cgccccgggc ccgggccagc tcgcaggccc gggcgatgtg ttcggcgggc ccggaaatga
    16261 ccaactgccc ggggcagttc aggttggcca gcgtcacccc ggcctgggcg cagacctcgc
    16321 gggtgggggt ctcatccagg ccgatgatgg ccgccatgcc gccgcgggtg gccgcgcagg
    16381 cttcctgcat gaaacgcccc cgctggcgca cgaggttcaa gccgtcttca aagcccagcg
    16441 cgcccgccgc cgtcagggcg gtgaactcgc ccagcgacag gcccgcggcg gcctggaagc
    16501 tcagccccgg cacgcgctcc cgcagtaatt gcagcgccac ccagctcacg aggaagatgc
    16561 ccggctgcgc gttctccgtc cgggtcagct cggcttccgg cccgtcaaag caaagcgcgg
    16621 ccaggtcgta gcccagcgcc gcgttggccc ggtcaaacca gctccgggcg gtgggcagtt
    16681 cccgcgccca atcccggccc atgcccacca cttgcgcgcc ttgcccggca aataataatg
    16741 cggttttaat cataagcggc tgccactaaa caccaaaccc gccccgctgg aaagccggga
    16801 gatgcccccg ccgccggggg cggcggcgcg ggaaaccggc gcgagaaacc ggttgacgcg
    16861 ggccgggact ttggcagaat gcccggcatt agggttatga accagtccgg cgacagactc
    16921 cgttttctcg cgcagcgcag cgtccactgc gtcgtgtgcc agcgcgcgcg ccgccagcag
    16981 gcggggctgg cgttctggct ggtccggacc ttcgcgcgcc tgtgcccgtt ttgccgggcg
    17041 tatgcggtgg tctatggccg gaaatcctac gaaccggaac cggcccgccc ggctcccacc
    17101 ggggcgcgcg aacgcgcccc ccttgtttag tcatccgcaa aacctatgaa ccgaatcctc
    17161 tccgcccaca ctctgttccc gctcaccgcc gcgctgctcg gcgccggcgt tctcatcagc
    17221 gccgcgcagg acgccgcccc gcgcctcgtc gtcccggacg gcaaggccta caaaggcagg
    17281 aaaatcatcg cccgggtcta tgactcccac tttgccctgg ggcacgacag ctacaactcg
    17341 atgggcaccg gcagcgacgg gaagatctac tacgtcctga gctcggagaa tattgaccag
    17401 ggcgccaaga tgttctgctt cgatcccaag acccagcaga tcaaggaagt gggcgacgtg
    17461 acggaagcct gcggcgaaaa gggcagcaag gccattcccc agggcaagag ccacgtcaac
    17521 ttcgtcgaga aggatcataa gctctatttc gccacccacg tcggggtgta cagctacgtg
    17581 gacggcaagg aaaccatggg catccccccg gcgggctata aaccctaccc gggcggccac
    17641 ctgctctcct acgacctgaa gaccggcaag ttcgaggact acggcatctg cccggagcgc
    17701 gagggcgtgc tcaccatgaa catggacacc cgccggggcc gtctctttgc cctgacctgg
    17761 cccagcggca tcttctaccg ctatgacctg gccacccgca accagaagag cttcggcaaa
    17821 atgtgcgccg agggggaaaa cggcaccggc ccggcctacc gcaccgtctg ccgctccatc
    17881 gtcgtggacc cgcgggacgg ttccgcctac ttcacctcct ccgagggcac catcttccgc
    17941 tacgatgccg cgagcgactc cgtgagcgcg atgaagggag aaaccatggt caaggattac
    18001 ttcggcagct acgaccccac cgacgccggg cacatgggct acaactggcg ccagaccttc
    18061 tggcgggagc aggacaacct catctatggc gtccacggca actcgggata cctgttccgg
    18121 ttcgaccccc gcgtgccgcg cctcgaagtc atgcagcgca tcacctccga accctccaag
    18181 cgcagcggca tgttcgacca gttcagctat ggctacctgg cctttgccct cggccccgac
    18241 ggcaagacca tccattacct caccggcgct cccatctaca ttgacggcca gcgcctcgcc
    18301 ggcaaggcca aaaccgcgat gggcgaagcc aaaggcctcg aagacctgca cctcatcacc
    18361 tacgacctca agaccggccg atacaccgac cacggcgccg tcttctaccc cgacggccag
    18421 cgcccgctct acgtcaacgc catggccatc ggcaaggacg gcatgatcta taccctcccg
    18481 cgcatcacgg ccaacggcaa gacccgctcc gacctgatcg ccttccccgg cccgctcaaa
    18541 aaatagcgcc ccgcccgccg cagccaccgc acggcaacgg ggccgcgctc aattgagcgc
    18601 ggcgaaaggc gcctgaatca agggctgcca ggcgagttgc gcgttccctg cgtttggggg
    18661 aacctcagcg gcgtcgcagc acggcctcgc cgatgatgcg cgcggcttct tcgtacggca
    18721 catcatcggt gttgatgatg aggtggtaga gcaggggatc gtcggggtcg gcgtcgaaat
    18781 actttcgcag gtagcgtttg cgcccgcggt cttcctgcag aatccgcgcc cgcgccaccg
    18841 ggcgggtgaa atgatccagc tcctgcacgt gtttaatccg cttctccagc gagccgatga
    18901 tgcggacccg gaagacgtag gggagcttgg cggtgatcac ggtggccccg cggccaatca
    18961 ggatcacgtt gcccagctcc gccaggcgga gaatggtgtc ggtggttttg tggaccagcg
    19021 tccaggaggg gggatgcaga tcgaacagct cgttcatgat gtccgtgatt tcggaggcgc
    19081 ggtcttccgg catgaaccgg gccaggcgct ggggcaaatt gtggtcttcg aggactttct
    19141 ccacaaggtt gcgatcgaac accgcccacg ggcaggcgtc tttgggttcg tgcgcgtgca
    19201 ggtaggctgc caggttctgg gccacaatat gcgcgcccga gccggtctgg cgggaaatgg
    19261 tcacggcgcg ccgcggcgcg tccgtttcac gcgcggtctg ccccgcgcct cccggctgca
    19321 actggcaatt gatgaaactc aaagcccgct ctaatccagc ttgtgtcttc atgtgtattt
    19381 tttgcttcct gcgtccgttt ccatttcgcg tcccgtctgc cgtgcgctct caagaaacgt
    19441 taccacggtt gggccggcaa gggaagcctg ttcctgcgcc gggcggccgg ctccgcgttc
    19501 ggcggtttcc gttcgcgcag ccgccccggt tttccggggc cgcggccgta tagcctccgg
    19561 ggggcgggct tatggcggcg gtggacgtcc tgtgcttttc cgggacacac tgtctgaaaa
    19621 actttcgccg cccgcccggc ggggtcgcgc ggaggcgggg ctcggggccg gggcgcgcgc
    19681 cgtcagcccg tggtggaacc tccaaaacgc ggccgaataa tccgtttaac cgcgtccgcg
    19741 ggcggggcgt gtctcattta tagccaggcc gccgcattgg cgcggtttgg gccggcggct
    19801 ccggcggagt tggcagcatt tatgcgttga gcgggttagc gtatgtcgca ctcagtattg
    19861 ttgttcttcc ggaagcgcgc ggtgtccctc agcctgctgg ggggcctgct cggcgttctg
    19921 gccccgcggc cctctctctt cgccgcgagc gtgacgctga actgggacgc gagcccgagc
    19981 cccgatatcg tccaataccg ggtttactac ggcgtggcca gtgccgccta cacccattcg
    20041 gttccggctg gcaccaacct gacggtgtcc atttccaatc tcgccggggg gcttacttac
    20101 tatttcgccg ccaccgccac cgacatcaac ggcacggaga gcgattattc cgcggagatt
    20161 gcggcactca ttccgctgaa ccaggccaac cggccgccga ccttgaacgc cctggccaat
    20221 ctgacgctca acgagaatgc cggtccacag acggttaacc tcctgggcat cagctcgggc
    20281 gccgccaatg agtcccagac gctgaccgtt acggcttcgt ccagcaaccc gggcctgatt
    20341 ccgaccccga cggtgaacta caccagcccc aacgcctccg gcaccctcag cttcaccccg
    20401 gtggcctatg cctacggctc cgccacgatc accgtcaccg tcaacgatgg cgccaccgtc
    20461 aacaacctca tctcccgctc ctttacggtc acggtcaatt cggttaacca gccgccgacg
    20521 ttgaacgccc tgcccaacct caccctcaac cagggtgcgg gccggcaaac ggtcaacctc
    20581 tccggcatca ccgcgggggc gcccaacgaa tcccagacgc tgaccgttac ggcctcatcc
    20641 agcaatcccg gcctgatccc gaccccggcg gtgaactaca ccagccccaa caccaccggc
    20701 tccctcagtt tcaccccggt ggccaccgcc agtggtgccg ccacgatcac cgtcatcgtc
    20761 aatgatggcg gcgcgagcaa caacctcatc gcccgcgcct tcaccgtcac cgtcaacccg
    20821 gccaaccagc cgccgacgat ttccgccctg gccgactacg tgatggcggc caacaccacc
    20881 acgccgccca tccccttcac catcagcgac ccggagacgg acgcagcgag cctgacgctt
    20941 gcgggcaatt cggataaccc gggcctggtg ccggtttcgg gcattgtctt cgggggcagc
    21001 ggcagccacc gcacggtcac cctcacgccc cgcccggggc aaatcggcag cgcccatatc
    21061 accctcaccg tcagtgatgg cgcaaacacc ttcagccggg tatttctgct gacagttcgg
    21121 ctgcggccgg aagcccccgt ggggttccat accgttagcg ccgggttcta acggcccggc
    21181 ccccaatcat tctcatccgc ccccggcgcg cgggcatcct gcccgcttag ccgcccctca
    21241 agcagaaagc ctcccacccg cccacccgct gaccgacaag attttcgtgc ggccatgaaa
    21301 tgtcgtgcta gcgctgcatg cgtgcgcgcc gcctgccagc gcctccttct atcttcagaa
    21361 ggccgcatgc ctccacgcgg cgctttcatc cccgcctctc ccggcgcgcg ggcatcctgc
    21421 ccgcttagcc gtccctcaag taaaaagcct cccacccgcc cacccgctga ccgacaaggc
    21481 tttcgtgcgg ccataaaatg ccgggctaac gctgcatgcg tgcgcgccgc ctgccagcac
    21541 ctccttccat cttcagaagg ccgcgtgccc ccacgcggca ctttcacccc cgcctctccc
    21601 ggcgcgcggg catcctgccc gcttagccgt tcctcaagca gaaagcctcc cacccgccca
    21661 cccgctgacc gacaaggctt tcgtgcggcc ataaaatgcc gggctaacgc tgcatgcgtg
    21721 cgcgccgcct gccagcgcct ccttccatct tcagaaggcc gcgtgccccc acgcggcgct
    21781 ttcatccccg cctctcccgg cgcgcgggca tcctgcccgc ttagccgttc ctcaagtaaa
    21841 aagcctccca cctgcccacc cgctgaccga caaggctttc gtgcggccat aaaatgccga
    21901 gttgtgtccg tcaagttcag caaattggtt atcatggttt ccactcaagg tg
//
LOCUS       DAOPQG010000060        21288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3069_length_21288_cov_13.797956, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000060 DAOPQG010000000
VERSION     DAOPQG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 21288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21288
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3069_length_21288_cov_13.797956"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..1142)
                     /locus_tag="PKI07_08315"
     CDS             complement(435..1142)
                     /locus_tag="PKI07_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51094.1"
                     /translation="MRLGAFTLIELLVVIAIIAILAALLLPALASAKEKSMRAACKSN
                     MRQAIITVHLYGMDWQEKVPSGRDNNDQWHSLRISSRSWTNLVDYTKNVKILDCPSFT
                     FGTQPRFSSTYGYLIGYNYLCDANMSGWLPSRLEYWYSARKTTESGTNYVLADANHWG
                     GGSGTYLIMAPHCKTGPYRPNGATWTWGKGTETSQSVGASGGNVGRLDGSVAWVGINS
                     LRKRYASGSYIMYYGLW"
     gene            1682..2341
                     /locus_tag="PKI07_08320"
     CDS             1682..2341
                     /locus_tag="PKI07_08320"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC51095.1"
                     /translation="MRRKPTVDPQTRILDAAENAVAECGFAGASLRRIAQEARVNLAT
                     VYYYFRSKRGLVEAVLKRRFGPLREEHLALLREFEAANPGRPLPVEKILEAMLVPPLR
                     LAAQDSARRQAVRRLFGRIVGEPNAETQEMLRHLRMESRTAFLKALQRSLPEAPRADL
                     LWSVEFVSGALAFTLCNPRELEVETRGACNPANTEKVLAEMIRFFSAGFHALGRRPRK
                     G"
     gene            2412..2684
                     /locus_tag="PKI07_08325"
     CDS             2412..2684
                     /locus_tag="PKI07_08325"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOC51096.1"
                     /translation="MSRRPLHLVNFFCMAPQAKSVSLVGDFNGWQPAAHPMTRMPDGG
                     WTIRLELPHGHHQYVFLVDGQPTLDPNAMGKVHNERNEVVSLIAIS"
     gene            2710..3963
                     /locus_tag="PKI07_08330"
     CDS             2710..3963
                     /locus_tag="PKI07_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC51097.1"
                     /translation="MIPSRLTHPSPTPKLRPAVRRARLRAGFLLPAAGAVLLAGCQRQ
                     APPPPPPVPEVAVVTVSRQPILLTTELPGRTAPFLIAEIRPQVNGLILKRLFTEGTDV
                     RQGQVLYQIDPAPFQAALDNAQAALGRAEANLPAIQSRVDRYRQALADKAVSQQDLDD
                     AEAALRQAKADAAYYKATLQTAQINLGYARVTSPISGRIGTSAVTDGAIVTAYQPVPL
                     ATIQQLDPIYVNVPQAATDLLRLEQRLKAGQLKRNGANLNKVRLFLPDGSKYAQEGTL
                     QFQDVSVDPTTGSVILRMVFPNPDGVLLPGMFVRGVVQEGVNEQAILIPQQTVARDPK
                     GHPVALVVGADGIVQQRELTLDREIGDQWLVSAGLEAGDQVIAEGVQKVRPGVSVKTV
                     PFEQKPAPGKAGPVAAAAAAVEKSN"
     gene            3976..7170
                     /locus_tag="PKI07_08335"
     CDS             3976..7170
                     /locus_tag="PKI07_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC51098.1"
                     /translation="MLSRFFLKRPVFAWVIAIILMVAGCLAIYNLPIAQYPPIAPPSI
                     AIQAFYPGASAETVENSVTQIIEQKMTGFDNLLYLSATSDSAGACRLELTFTPGTDPD
                     LAWAQVQNKLQLAMANLPDVVQRSGIKVSKSTRNYLMIVSLISEDGSMDGYDLRDYAQ
                     SNLEKVLSRVPGVGEVEVFGGQYAMRIWVNPDKLTEYQMTMEDVIAGLRTYNVEVSAG
                     QFGGAPALPGQRLNAAIIVQSLLKTPDEFAAIPLRTSPDGSMVRIRDVGRVELGTDAY
                     DIEAFYNGKPSSAMAIRQAAGANALQTADNVRNKLAEMSHFFPSGMKVVYPYDTTPFV
                     RVAINEVVKTLIEAIFLVFLVMYVFLGNMRATLIPTIAVPVVILGTFAVLGIFGFSIN
                     MLTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLPPFEATRKSMDQITPALIGIGLVL
                     SAVFGPMAFFAGSTGVIYRQFSVTIIAAMLLSVVVALVLTPVLCATFLKPVAKGHQPA
                     EGGVWFLRPFFRWFDRVFFRARNRYLALVGHSLNRKLRYVLVFLVIVGALGYLFKRMP
                     TAYLPDEDQGMMMVQALLPANSTLEQTKEVMANVRDYFLTQEKEAVHAVMTVSGVSFS
                     GQGQNSGLAFVRLKDWDLRDRPELKVTAVAGRAMMRFSQFRNAMVFAFAPPAVVELGQ
                     AKGFDFQLLDRGGLGHEKLMQARNQLLGMAAMNAASTNGAVLTKVRPNGLEDVPEYRV
                     DVDWDKAGALGVPITSIHNTIAAAFGSAYVNDFIQGGRVKRVYAQADAPYRMLPRDLE
                     KLYVRNKKGQMVPFASFASGHWTSGSPKLERFNGFPSLNILGESAPGRSSGEAMRMME
                     SFVRKLPQGIGYDWAGISYQERQAGAQTLPLYAFSVLVIFLCLAALYESWPIPLSILL
                     TLPLGAIGGVIASSLLGLPNDVYFQIGLLTTLGLTTKNAILIVQFAKARFEEGDGLIA
                     ATLEGAKLRLRPIIMTSLAFGFGVLPLALAHGAGAGAQRAIGIAVVGGVVTSTFLVTL
                     FAPLFFVVIEKGIGKQSRRAAQAARAASSESPAA"
     gene            7176..8642
                     /locus_tag="PKI07_08340"
     CDS             7176..8642
                     /locus_tag="PKI07_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOC51099.1"
                     /translation="MNKRIRLLLPGIVLVLAGCTLAPKYTQPAAPVPDAWPTGPAYTA
                     AAPTNAVVNPDLNWLEFFPDEKLRQVIRLALANNRDLRVAALNVERARAYYGISRAEL
                     YPAVHAAGSGSKARVPADLSPSGRRQTVERYEANLGVAAWEIDFFGRIRSFKDRALEE
                     YLATEQARRSAQILLISAVAKTCLALAADREALALAQTTLQTQQAAYDLVQRRYQLGL
                     TTELDLFRAQTPLEAARRDVALYTQQAAKGENALALLTGAPVESDLLPAGLDDLLPPE
                     EISAGLSSQVLLLRPDILQAEHLLRAAHADIGAARAAFFPRISLTAAIGTASRDLSGL
                     FQAGSGTWSYAPQIVMPIFDARTWAAARAAKVQREIAVTQYERAIQNAFREVADALAV
                     RGTVDQQITAQQALVHASAETYRLAHSRYEKGLDSYLGVLDAQRSLFAAQQALVSLRL
                     AKLTSQVQLYTVLGGGWQTQELAGAPPLSDPVREPKGN"
     gene            8791..9291
                     /locus_tag="PKI07_08345"
     CDS             8791..9291
                     /locus_tag="PKI07_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOC51100.1"
                     /translation="MSNTSNNLKEAFAGESQASQKYLAFAKKAEKDGFRNIAKLFRTA
                     VEAERIHAEAHLSAMDGIGSTADNLKTAIAGETYEFTTMYPPMLKQAEAENHKAKRMF
                     DLAPKAEAVHARLYQMALEAVQRGEDLPQDKIYLCPICGDIEFGTPPESCPICKAKGS
                     VFIQVQ"
     gene            complement(9415..10179)
                     /locus_tag="PKI07_08350"
     CDS             complement(9415..10179)
                     /locus_tag="PKI07_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51101.1"
                     /translation="MICIVCIVISVVLGLTLIWGTQDNPAFSQGEKMHTLPLQFRERH
                     LFLELDGDLWLLDTGAPASFGTPRGLTLAGEPFNFGANYLGLTAETLAQFVGMPCVGL
                     LGADVLGRFDHLFDIAGGSLTVSTDELPHNGQTVRLDMFMGIPIVTARISGRDYRMFF
                     DTGAQISYFQDDSLAAYPPAGRVTDFYPGFGQFQADTYHVPAALGGVGFTLRCGALPR
                     LLATALMQMNTEGIVGNAILRDRKVGYFPRRRLMAL"
     gene            complement(10890..11629)
                     /locus_tag="PKI07_08355"
                     /pseudo
     CDS             complement(10890..11629)
                     /locus_tag="PKI07_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015112036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(11626..12537)
                     /locus_tag="PKI07_08360"
     CDS             complement(11626..12537)
                     /locus_tag="PKI07_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOC51102.1"
                     /translation="MRIPHPIPYQGSKRNLATEILRYFPERATTRIEPFAGSAAITLA
                     AAARGLAAHDVINDLNKPLVDLWRAIVESPEKLTRQDESLWRAQQENRREYYDKVRDA
                     FNRTGRPDYLLYLPARCVKASVRYNADGKFNQSPDNRRLGALPATLREHILGASHLLH
                     RKTEFSSLDYREVVAQATPNDLIYMAPPYQGVCGERDPRYLKGVLVDDFVEVLESLNY
                     REINYLVSDDGRTGGRVHGRKLPDRLKLHLLELPAGRSSQATLLGRAEVTVESLYLSP
                     ALAESLRLRRHYPRRAAEQLAFLEARR"
     gene            13076..14530
                     /locus_tag="PKI07_08365"
     CDS             13076..14530
                     /locus_tag="PKI07_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOC51103.1"
                     /translation="MNGVKQPVLVPVITAGLVGFACVAGAAERGPKERRAQTRPPNIV
                     LVLADQWRAEAFGYAGNPDVKTPNLDRLQREGIHFVNAVSGMPVCCPARASFLTGQRP
                     LTHGVFLNDVPLNPEAVTIAKVLGAAGYDTAYIGKWHVDAHGRSSFIPRERRQGFDYW
                     KVLECSHDYNHSAYYADGPEMLYWEGYDAIAQTRDAQQYLRDHAPPRKPFFLVLSWGP
                     PHDPYFTAPEKYRALYDPAKLTLRPNVPEAEAAQARKILAGYYAHCTALDDCFGELRR
                     TLRETGLEETTLLVFGSDHGDMLGSQGFFKKQRPWDESIRVPLVLRWPGGLGTRARLL
                     EAPFNVEDLMPTLLGLCGVPIPKTVEGLDYSGYLRGRKNPGDNATVILCASPFGEWER
                     RKGGREYRGIRTPRHTYVRDLNGPWLLYDNQADPYQTNNLANQAKHARLQARLDAVLT
                     RKLKERHDAFLPGNAYIQQWGYQVDANGTVPYKH"
     gene            15146..15331
                     /locus_tag="PKI07_08370"
     CDS             15146..15331
                     /locus_tag="PKI07_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51104.1"
                     /translation="MEERTGVDENVLFPKFEKLKTEEVRGARDTFHGRMRINTRGHYQ
                     NFAGAILEVGQHLKAQG"
     gene            15333..18536
                     /locus_tag="PKI07_08375"
     CDS             15333..18536
                     /locus_tag="PKI07_08375"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOC51105.1"
                     /translation="MSAASRGGELFIVDNSDTDWKGLRYLQDWTEIASAFDIATGFFE
                     IGALLALDGKWQKLDRIRILMGGQTTARTRQAILEGLRRHVREKLDGSLEREKESNDF
                     LGGARAIVEAMKAGKIQCRVYAKRKFHAKAYITHPRVAVIGSVALVGSSNFTVPGLTE
                     NVELNIQIRAPGDVARLQEWYEQHWQEAEEVMPDVIRVIERHIDEYPPFEVYAKALQE
                     LFEHHTPTEAEWENGASKMYPVLDHYQKEGYHSLLNIARQHNGAFLCDGVGLGKTFVG
                     LMLIERLVMHDKKRVVLLVPKSGRVAVWERSIRRYLPHLLNGFLPFKIYNHTDLMRGP
                     SGDGRDFPAEFQQIKEQADVVLIDEAHHFRNRGLANAEDGTIRSRYWQLYDIIGQKTV
                     FLLTATPVNNALTDFQHEIELFSRVDNPTAFAGALGIHNLASHFQQLEKALKRLTAGE
                     EHGELFQVNQAEAEKVLFDDKLFRSLVVQRSRAYAQQSQLQHGGTQAMFPTKEPPKVQ
                     PYSVKKTYGHLLGKVEAAFSKPDQLFSLAVYYPLGHWIGDPSALRSFDVNRQKQVVRL
                     IRIQFLKRFESSIYAFETSCQNLLLKLLAFLRKNSTTPTEVKRLHRWEAQNAELLEHV
                     KERRSELQEEDESEEADTSELGDEFLDDFEELSRTDYNLPEIFDETYADLEQLVDFLE
                     ELKTFNASHDNKLQSLIKLLKGDAVLKKQKVLIFSEFMATARYLRRELLKAGIEGVEE
                     IDSASTIDRGDAIQRFAPYYNGSSSGELAAKGHKETRILISTDVLSEGLNLQDATRLI
                     NYDLHWNPVRLMQRIGRVDRRLNPEIEAKMVADHPDLKPLRGKVIYWNFLPPEDLDGL
                     LRLYHRVSSKTLRISKVFGIEGRKLLKPEDDFDALKDFTHKYQGELTPLEKMHLEFQK
                     LLADNPGLQDRLNGLPGRVFSGREHPQAGPKAVFFCYALPAEAKGLDGSGADKTESEK
                     QNTETGGDAPAWTTEARKAAWYLYDLAGGKILERAEEIVAFVRSGPETARRVILPETE
                     LRDARLRVEKHIKNTYLKQVQAPVGVKPVLACWMELN"
     gene            18541..19626
                     /locus_tag="PKI07_08380"
     CDS             18541..19626
                     /locus_tag="PKI07_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOC51106.1"
                     /translation="MMTLRQFAAEPATLPMRATWYLTDLAEAKGRHELYTRQSPQKLK
                     VLREHALIESAVSSNRIEGVEVDASRVATLMFGKPALRDRDEEEVRGYRDALRLIHES
                     SARLAISEETIKRLHHLCRGQIWDAGAYKEKDVDIIQTYAEGRSRVRFKTVPASQTPA
                     AMAELVELWQRGMAETWAPPIVLAAALNLDFLCVHPFRDGNGRVSRLLLLLATYHCGI
                     EIGRYISLERLIEENKERYYEVLEQSSQRWHEGKHDPWPAMNFFLFILAQAAREFEQR
                     LGQIKTPKGAKTALIEHAIDALGEFGIADLERRCPAVSRDMIRHLLRTLKAAGRVECL
                     GRGPGAKWRNKGNTPKGGKNKGNNQGN"
     gene            19879..>21288
                     /locus_tag="PKI07_08385"
     CDS             19879..>21288
                     /locus_tag="PKI07_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51107.1"
                     /translation="MSNQLDLERLRSLKTLPQLIAYLRDELDWPIDQNAVADDYIFEY
                     RPEELGFDADAAVRIREIKQLRPLEKGQPWGIFWVSFAKKRLPVVMLRRILGHLVLKK
                     RAAAQKASQRAWHLHDLLFISAYGEDSDRAVSFAHFSQEPESPGDLPVLKVLGWDDGD
                     TVLHLADAHRTLAEKLRWPGKPVNLKAWREQWSSAFTLRPREVITTTTQLVEELARLA
                     EATRRRANSILARESEHGPLRKLCAAFRAALIHDLTEDDFADVIAQTISYGLLIARFS
                     RAAISAQNLVDMVPQTNPFLRELLAAFLALAGRQKAFDFDELGIQDVVELLNQANADA
                     VKADFGNRTRNEDPVIHFYEHFLAAYDRRKKIQRGVFYTPQPVVSYIVRSVHELLQKE
                     FDIEDGLASTVTWADMLKRWPDLRLPKIRVPKVGQAEGVEVALAADEFFVMILDIATG
                     TATFLVETIEVIFQHLKAKW"
BASE COUNT         4015 a         6556 c         6924 g         3793 t
ORIGIN      
        1 cggttggcgg ggacattccc cttcccaggc gagggttgcc gcaaggtgac tgcttgcgtt
       61 gtcctcaggg ttggccggcc gttttgaagc gatagacgct ttcatccccg ccccctgaac
      121 ccgaaaagtg gctttgggtt tgggaaaacc ttttcccctt cagctaccaa aacgcaggcg
      181 ctgataacca aaggctctat acctgagcca gaacgatctt gattgcgccc tcattccacc
      241 ttctctgtgt gcgtaagtct ctgattttac aggcggaagg cgttcggaac ggaacggttg
      301 ccctgtggga ttcatcagcc caggggttta ttggagttgt ggatcggatg accaggggta
      361 tcccggctcc aatggaatgt ggccgcgcgg ttgagcggcc caatggcagc ggttcggtta
      421 aggcggcgcc gttgtcacca gaggccgtag tacatgatgt acgagcccga agcgtagcgt
      481 ttccgcaagc tattgatgcc cacccaggcg accgagccat caagccggcc cacgttgcca
      541 ccagacgcac cgacgctctg ggacgtttcg gtgcccttgc cccaggtcca ggtcgcgccg
      601 ttggggcggt aagggccagt cttgcagtgc ggggccataa tcaggtaagt gccactgccg
      661 ccgccccagt ggtttgcgtc cgcgaggacg taattggtgc ccgactcggt ggttttccgg
      721 gccgaatacc aatattccag ccgggaggga agccagcccg acatgttggc gtcgcaaagg
      781 taattgtagc cgatcaggta gccgtaagtg gagctgaacc gcggttgcgt gccaaaggtg
      841 aagcttgggc aatcgaggat tttcacgttt ttggtgtagt caaccaggtt ggtccagctc
      901 cggctggaga tgcgcagcga gtgccactga tcgttgttgt cgcggccgga gggaaccttt
      961 tcctgccagt ccattccata caggtgaacc gtgatgatcg cctgccgcat gttgcttttg
     1021 caggccgcgc gcatgctctt ttctttcgcc gaggccagcg ccgggaggag cagggccgcc
     1081 agaatggcaa tgatggcaat aaccaccagc agttcgataa gggtgaatgc gccgaggcgc
     1141 attcggcctg gtctgtgttt taaggaaggt tgcataatcg tcagggggga tttatccgga
     1201 atgagcccgg ccgcgcccag tgccgtgtcc gcatcattgg gccgcgaggt tccccgggtt
     1261 ggcccggcaa cccatcggcc ggcatcggcc acgcggcctt gccgcccgct ccgtccgccg
     1321 ggcaaagcct cttcgggcgc cgttcctcag cccggaaaac agcctttccc tgaatcccgt
     1381 ggttccggac acggttttcg gccccaggat tcctcatgcg cactgtgcgc ggcataccct
     1441 ttccttaccc gcgcgccccg ggagtgtcaa tcgcaaaggc ggctccccgg ttcggctcgc
     1501 cggtggggtc cgcgggcggc gggccgcgta ttccggtccg ggcggccgca acccgggggg
     1561 cgaacctccg ggcggccggg cccgcagccg ccggggggcg caaaaaaatt aggggatacg
     1621 atttgacagc cggtgggcgg cggcgtaatt taacgcccgt ttagaaagaa cgtttaatta
     1681 gatgcgccga aaaccgacag tggatccgca gacccggatt ctggacgccg ccgaaaacgc
     1741 ggtggccgag tgcggctttg ccggggcgtc gctgcggcgg attgcgcagg aggcgcgcgt
     1801 gaacctggcg acggtgtatt actacttccg ctcgaagcgc ggcctggtgg aggcggtgct
     1861 caagcggcga ttcggcccgc tgcgggagga gcacctggcg ctgctgcggg agttcgaggc
     1921 ggcgaacccg gggcgcccgc tgccggtgga aaagattctc gaggcgatgc tggtgccgcc
     1981 cttgcgcctg gcggcgcagg attcggcccg gcgccaggcc gtccggcgtt tgtttggccg
     2041 catcgtgggg gagccgaacg cggaaaccca ggagatgctg cgccacctgc ggatggagtc
     2101 gcgcaccgcc tttctgaagg cgttgcagcg cagcctgccg gaggcgcccc gggccgattt
     2161 gctgtggtcg gtggagtttg tttcaggcgc gctggcgttc accctgtgca acccccggga
     2221 gttggaggtc gagacgcgcg gcgcctgcaa tcccgccaac accgagaagg tgctggccga
     2281 gatgatccgg ttcttttccg cggggttcca cgccctgggg cggcgcccac gaaagggttg
     2341 aagcgcgcct ggctgtcatg gataccgctt tttaccaacg ccggtcaccg gccgccccgg
     2401 ccgccccggg aatgtcgcgc cggccgctgc acctggtgaa tttcttttgc atggcgcccc
     2461 aggcgaaaag cgtgtctttg gtgggcgact tcaacggctg gcagccggcg gctcatccga
     2521 tgacccgcat gccggacggc ggctggacca ttcgcctgga gctgccccac gggcatcatc
     2581 aatatgtttt cctcgtggac ggccagccga cgctcgatcc caatgccatg ggcaaggtcc
     2641 ataatgaacg caacgaggtc gtctcgctga tcgccatcag ttagcccatc cccggccaga
     2701 ttttaattta tgatcccctc acgactgacg catccttctc cgacaccgaa actccgcccg
     2761 gctgtgcgcc gcgcccggct gcgggccggt ttcctgctcc cggccgccgg cgccgtcctg
     2821 ctggccggct gccagcgcca ggcgccgccg ccgccgcccc cggtgccgga ggtggcggtc
     2881 gtgacggtga gccggcagcc gattctgctg accacggagt tgccggggcg cacggcgccg
     2941 tttttgattg cggaaatccg gccgcaggtt aacgggctga tcctcaagcg cctgttcacg
     3001 gaggggaccg acgtccggca agggcaggtg ctttaccaga ttgatcccgc tccgtttcag
     3061 gcggcgctgg acaatgccca ggccgcgctg ggccgggcgg aggccaacct gcccgccatc
     3121 cagtcccggg tggatcgcta ccggcaggcc ctggcggaca aagccgtcag ccagcaggat
     3181 ctggacgatg cggaggcggc cttgcggcag gccaaggccg acgcggcgta ttacaaagcc
     3241 acgctgcaga ccgcgcagat caacctgggc tacgcccgcg tgacctcgcc catctcgggg
     3301 cgcatcggca cttcggcggt gacggatggg gcgattgtca cggcttatca gccggtgccc
     3361 ctggccacga tccagcaact ggaccccatt tatgtgaatg tgccccaggc cgccaccgac
     3421 ctgctgcggc tggagcagcg gctgaaagcc ggccagctca agcgcaacgg ggccaacttg
     3481 aacaaagtgc ggctctttct gccggacggc tcgaagtatg cgcaggaggg gacgctgcag
     3541 ttccaggacg tctcggtgga tccgaccacc ggctcggtca tcctgcggat ggtgtttccc
     3601 aacccggacg gcgtgctgct gccgggcatg ttcgttcgcg gggtggtgca ggaaggcgtc
     3661 aacgagcagg cgatcctgat cccgcagcag accgtggcgc gcgatcccaa ggggcacccg
     3721 gtggcgctgg tcgtgggggc cgatggcatc gtgcagcagc gcgagttgac cctggaccgg
     3781 gaaatcggcg accagtggct ggtcagcgcg gggctggagg cgggcgatca ggtgattgcg
     3841 gagggcgtcc agaaagtgcg ccccggcgtg agcgtgaaaa cggtgccctt tgagcagaag
     3901 ccggcgccgg ggaaagccgg gccagtggcg gcggcggccg cggcggtcga aaaatccaac
     3961 taagcaggac gccccatgct atcaaggttc ttcctgaagc gcccggtttt tgcctgggtg
     4021 attgccatca tcctgatggt ggccggctgc ctggccattt acaacctgcc gattgcgcag
     4081 tacccgccca tcgcgccgcc gtccatcgcc atccaggcct tctacccggg ggcctcggcc
     4141 gagacggtcg agaacagcgt cacgcagatc atcgagcaga agatgaccgg gtttgacaac
     4201 ctgctctacc tttcggcgac cagtgattcc gccggcgcct gccgccttga gctgaccttt
     4261 acgccgggga cggatccgga cctggcctgg gcgcaggtgc aaaacaagct ccagctggcc
     4321 atggcgaacc tgccggacgt agtccagcgc tccggcatca aggtgagcaa gagcacgcgc
     4381 aactacctca tgatcgtcag cctgatctcc gaggacggca gcatggacgg ctacgatttg
     4441 cgcgactacg cgcaatccaa cctggagaag gtgctgtcgc gggtgccggg ggtgggagaa
     4501 gtggaggtct tcggcggcca gtatgccatg cgcatttggg tcaacccgga caagctgacg
     4561 gagtaccaga tgacgatgga ggacgtgatt gcagggctgc ggacctacaa cgtcgaggtg
     4621 tccgccggac agtttggcgg cgcgccggcg cttccgggcc agcgcctgaa cgccgccatc
     4681 atcgtgcaaa gcctgctcaa gaccccggat gagtttgccg ccattcccct gcgcaccagc
     4741 ccggacggtt cgatggtccg gattcgggat gtggggcggg tcgagctggg caccgacgcc
     4801 tacgacattg aagcctttta caacggcaag ccttcctcgg cgatggccat tcggcaggcg
     4861 gccggcgcca acgccctcca gacggcggac aacgtccgga acaagctggc cgagatgagc
     4921 cactttttcc ccagcggcat gaaggtggtg tatccctatg acaccacgcc cttcgtgcgc
     4981 gtggccatca atgaagtggt caagacgctc attgaggcga tcttcctggt tttcctggtg
     5041 atgtatgtgt ttttgggcaa catgcgcgcc accctgattc ccaccattgc cgtgccggtg
     5101 gtcatcctgg gcacgttcgc cgtgctgggc atcttcgggt tttccattaa catgctcacc
     5161 atgttcgcca tggtgctggc catcgggctg ctggtggacg acgccattgt ggtggtggaa
     5221 aacgtcgagc gcatcatgag cgaggagggg ctgccgccct ttgaggccac gcgcaaatcc
     5281 atggaccaga tcacccccgc cttgatcggc atcgggctgg tgctctcggc ggtgttcggc
     5341 ccgatggcct tcttcgccgg ttccacgggc gtcatttacc ggcagttttc cgtgaccatt
     5401 atcgccgcga tgctgctctc ggtggtggtt gccctggttt tgacgccggt gctctgcgcc
     5461 accttcctca agccggtggc caagggccat cagccggccg agggtggcgt gtggttcctg
     5521 cggccgtttt tccggtggtt tgaccgcgtc tttttccggg cgcggaaccg ctacctggct
     5581 ttggtgggtc attcgctcaa ccgcaagctg cgctacgtgc tggtgttcct ggtcatcgtg
     5641 ggggcgctgg gatatctctt caagcggatg ccgaccgcgt acctgccgga tgaggatcag
     5701 ggcatgatga tggtgcaggc gctgctgccc gccaattcca cccttgaaca aaccaaggaa
     5761 gtgatggcca atgttcggga ttactttctc acccaggaaa aggaagccgt gcatgccgtc
     5821 atgacggtgt ccggggtgag cttctccggc caggggcaaa actcggggct ggcgtttgtg
     5881 cggctcaaag actgggatct gcgcgaccgc ccggagctga aggtcacggc ggtggcgggg
     5941 cgcgcgatga tgcgcttttc gcagttccgc aacgccatgg tgtttgcctt cgcgcccccg
     6001 gcggtggtcg aactgggcca ggccaagggg tttgacttcc agttgctgga ccgcggcggc
     6061 ctgggccacg aaaagctcat gcaggcccgc aaccagctcc tgggcatggc ggccatgaac
     6121 gcggcgtcca ccaacggcgc ggtcctgacc aaggtccgcc ccaacggcct ggaagacgtg
     6181 ccggaatacc gggtggatgt ggactgggac aaagccggcg cgctcggggt gcccatcacc
     6241 tccattcaca acaccatcgc ggcggccttt ggcagcgcct acgtcaacga cttcatccag
     6301 ggcggccggg taaagcgcgt ttatgcgcaa gccgacgcgc cgtaccggat gctgccgcgc
     6361 gacctggaga aactctacgt ccgcaacaaa aagggacaga tggtcccctt tgcctccttc
     6421 gcttccggcc actggacctc cggctcgccc aagctcgagc gcttcaacgg ctttccgtcg
     6481 ctgaatatcc tgggcgaatc ggcgccggga cgcagctcgg gcgaggccat gcgaatgatg
     6541 gaaagcttcg tgcggaagct accccagggc atcggttacg actgggccgg gatttcctat
     6601 caggaacggc aggcgggcgc gcaaaccctc ccgctgtatg ccttttccgt gctggtgatc
     6661 ttcctttgcc tggccgctct gtatgagagc tggcccattc cgctgtccat cctcctgacg
     6721 ctcccgctgg gcgcgattgg cggcgtgatt gcctccagcc tcctgggcct gcccaatgac
     6781 gtgtatttcc aaatcggcct gttgaccacg ctggggctca ccacgaaaaa cgccatcctg
     6841 attgtccagt ttgccaaagc ccgtttcgaa gaaggcgacg gactgatcgc ggcgacgctc
     6901 gaaggcgcca agctgcgctt gcgccccatc atcatgacct cgctggcctt cggcttcggc
     6961 gtgctgccgc tggcgttggc acacggcgcc ggcgccggcg cccagcgcgc catcggcatt
     7021 gccgtggtcg gcggcgtcgt cacctccacc ttcctggtca ccctctttgc cccgttgttc
     7081 tttgtggtca ttgaaaaggg gatcggcaaa caatcccggc gcgccgccca ggcggcccgc
     7141 gccgcttcct ccgaatcgcc cgccgcctga cttttatgaa taaacgtatt cgcttgcttc
     7201 tgcccggcat cgtcctggtt ctggcgggct gcacgctggc cccgaaatac acgcaacccg
     7261 cggcccccgt cccggacgcc tggcccacgg gccccgcgta cacggcggcc gcccccacaa
     7321 atgcggtggt taacccggac ctgaactggc tggagttctt cccggatgag aaactgcggc
     7381 aagtcatccg cctggccctg gccaacaacc gcgacctgcg cgtggcggcc ttaaacgtcg
     7441 agcgggcccg cgcttattat ggcatctccc gggccgagct ctaccccgcc gttcatgccg
     7501 ccggcagcgg cagcaaggcc cgcgtgcccg ccgacctctc cccctccggc cggcgccaga
     7561 ccgtggaacg ctacgaggcc aatctcggcg tcgccgcctg ggagattgat ttctttggcc
     7621 gcatccgcag cttcaaagac cgcgccctgg aggaatacct ggccaccgag caggcccgcc
     7681 gcagcgcgca gatactgctg atctccgccg tggcgaaaac ctgcctggcc ctggcggccg
     7741 atcgcgaggc cctcgccctg gcccaaacca ccctccaaac ccagcaagcc gcctacgacc
     7801 ttgtccagcg ccgctaccaa ctcggcctga ccacggaact ggatttgttc cgcgcccaaa
     7861 ccccgctcga agccgcccgg cgggacgtgg ccctgtacac gcagcaggcg gccaaaggcg
     7921 aaaatgccct ggccctgctg acgggtgcgc cggtggaaag cgacctgctg cccgccggtt
     7981 tggacgacct cctcccgccc gaggaaatca gcgccgggct ttcctcccag gttctgctgc
     8041 tccggcctga tattctccag gccgaacacc tgctgcgggc ggcccacgcc gacattggcg
     8101 cggcccgcgc ggcctttttc ccgcgcattt ccctgaccgc cgccatcggc accgccagcc
     8161 gcgatctctc cgggctgttc caagccggct ccggcacctg gagctacgcg ccgcagatcg
     8221 tcatgccgat ctttgatgcc cgcacctggg cggcggcccg cgccgccaaa gtccagcgcg
     8281 agatcgcggt cacccaatac gaacgggcca tccaaaacgc cttccgggaa gtcgccgacg
     8341 ccctggccgt ccgcggcacc gtggaccagc aaatcaccgc gcagcaagcc ctcgtccacg
     8401 cctccgccga gacctatcgc ctggcccatt cccgctacga aaagggcctc gacagttacc
     8461 tcggagtcct cgacgcccaa cgctcgctct tcgccgcgca gcaagccctg gtctccctcc
     8521 gcctggccaa actcaccagc caggtgcagc tctacaccgt gcttggcggc ggttggcaga
     8581 cgcaggaact ggccggcgcg cccccgctct ccgatcccgt ccgcgagcca aaaggcaact
     8641 gaatcgcgcc ccttgccggc ccggggcgtg acccgcgccg ggtggcctcg cagctcgaat
     8701 tgcccgcccc tcgcgcggca aaaaggcttc ccttccggcg ccgggactgg caggcttggc
     8761 ccagccaatt aacttaacag tcaacgcatt atgagcaata caagcaacaa tctgaaagaa
     8821 gccttcgccg gtgagagcca ggccagtcaa aagtatttag cctttgctaa aaaggccgaa
     8881 aaggacggtt tccgcaacat cgccaagctc ttccgcactg cggtggaagc cgagcgcatc
     8941 catgccgaag cccacctcag cgccatggat ggcatcggct ccaccgccga caacctcaaa
     9001 accgcgattg ccggggagac gtacgagttc accacgatgt acccgcccat gctcaagcag
     9061 gctgaggcgg aaaaccacaa ggccaaacgc atgtttgacc tcgcccccaa ggccgaagcc
     9121 gtccacgcgc gcctttacca aatggccctg gaagctgtcc aaagaggcga agacctgccc
     9181 caggacaaga tctatctctg ccccatttgt ggtgatatcg agttcgggac cccgcccgag
     9241 tcctgtccca tctgcaaggc caagggatcc gttttcatcc aggtgcagta atcgtccccg
     9301 gctttcggac ggcggcgctc aaccggtgcg cggcgcttcc cccggcagcg cgcactgcgg
     9361 ttttgccgcg atgctgcccg ggatcgcctc caggggtgag gcgccccttg cgcgctatag
     9421 ggccatgagg cggcggcgtg ggaaatagcc aaccttgcga tccctcagaa tcgcgttgcc
     9481 cacaatgccc tccgtgttca tctgcatcag agccgtggcg agcaagcggg gcaaggcgcc
     9541 gcagcggaga gtgaacccga cgcccccgag cgcggcgggg acgtggtacg tatcggcctg
     9601 gaactggcca aagcctggat aaaagtcggt gactctgccc gcgggcgggt atgcggcgag
     9661 tgagtcgtcc tgaaagtagg aaatctgggc gcccgtgtcg aaaaacatcc ggtagtcgcg
     9721 cccgctaatg cgagccgtga cgatggggat gcccatgaac atatccaggc gaaccgtctg
     9781 gccgttatgc ggcagttcgt ccgtcgaaac ggtgagactg ccaccggcaa tatcgaaaag
     9841 gtggtcgaag cggccaagaa cgtctgcccc gagaaggccg acgcacggca ttccgacgaa
     9901 ctgcgcgagt gtctcggcag tgaggccaag gtaattcgca ccaaagttga acggctcgcc
     9961 cgcgagggtg aggccgcgcg gcgtgccaaa gctggcgggg gcgccggtgt cgagcaacca
    10021 cagatcaccg tccagctcaa ggaacaagtg ccgttcgcga aattgaagtg gcagagtgtg
    10081 catcttttcc ccttggctaa atgccggatt gtcttgcgtc ccccaaatca gggtaagccc
    10141 caaaacgacg ctgatgacga tgcacacaat acaaatcatg aagcatgatt ttgttaagca
    10201 cttttgggtt catcgattta gcgcgaggct cgcaatacct ggccaatact ggttgggaaa
    10261 gtgttcctgc taaacgggct agaatgagca agctggaagt tcattcctgg atttgcttcg
    10321 tattggctgg aagtgtaggc aagaccgcga aagcggtcaa cggaaaaaag ccctcctcag
    10381 accgtccacg gccccgccaa tcccgctttc aatatcccgg cgccacatcc cggatcatgc
    10441 acccgggcgc atctgaacct ggcgcaggca cgcatgctcc aacccgaggg gcgctccagt
    10501 cgtacccgtg ggcggcttgg gcgtctatat ggcacgcgct cacaggctga aaactgccgc
    10561 cgcctcagac ggcttgggcg aaggagcgcc agcaacggtg taatcggtgt agcgtccgga
    10621 ttcagaggga cgacgagttc acgcggcctt ttgcgcttgc tgcaacaggc gggtagattc
    10681 gaagggcgcg gaccctgaat gcccgttttg cagtcagcga ccgacacggc cggtgcgtaa
    10741 acgaaaaagg gcgcggagca ccggacttcg tcacgcatcg gcaagtccgg ttcattcgcg
    10801 atggggttga taccccgaag cttgcttcgg ctttatttgg gtttgtgaag tcatacttct
    10861 aaatgccttg gagcttgctc cggggttggt tacttcgccc tgacggtctt gaggtagtcc
    10921 tcgataattc ccttcacata ctcggggata ttcttctgca actccaaagt gcggcgctta
    10981 agccgctcgc aggattccac ttccgccccg gtccagagga tgtcggcgcg gcgcgcctcg
    11041 cgcatggcca catgggagta gtcctccggg aaggcccaat agcatcggcg gcagatttca
    11101 ggcttcttca gcttcagcca atttacgcag cgctcgcagg agcaggattt cgcacggttg
    11161 gctgaaccac acagccgcat gtagtcgtcg aggttgggct ccgcgcccgg cacatcgccc
    11221 tgaatcgcga agggaatgcg gtggtcaatt tgcaactcac gcccgggaaa cgcttccaga
    11281 tagatcgcgc actgttcacc gtgccgcgcc accagccttt ccttgagttt cttgctgagg
    11341 accgtccggc cagcctgttt cccggttcgg gtcttgcgcg ggtccccgaa acggtaggcg
    11401 gcaatcttgc gtccatcgct ccctttgacg cgaaacatct cgcggggatg ccgagttcct
    11461 tcacatcccg cacggcccgc ggcgggtggt tgtagccgta ctggtcctta agttcctgcg
    11521 tggtgatgaa gccgtgcttg agaatgtggt caataaccgt tttagggcgc ttagcggtga
    11581 cggccttgca caacttgatg aatgcgggcg gcagcttcgg cgggctcacc gcctcgcctc
    11641 caggaaggcc agttgttccg ccgcccggcg cggataatgc cgtcgcaagc gcagtgattc
    11701 agccagtgcc ggggagagat agagcgattc caccgtgact tccgcccgcc ccagcagcgt
    11761 cgcctgggac gagcgacccg caggcaattc aagcaggtgc agtttgagcc ggtccgggag
    11821 ctttcgcccg tggacacggc cccccgttcg gccgtcgtcg ctgacgaggt aattgatctc
    11881 gcggtagttc agcgactcca gcacctccac aaagtcatca accaatacgc ccttgaggta
    11941 acgcgggtcc cgctcgccac agacgccttg ataagggggg gccatgtaga tcaggtcatt
    12001 cggcgtggcc tgagcaacca cctcccggta gtcgagcgaa gaaaattccg tctttcggtg
    12061 cagcagatgc gaggccccga ggatgtgttc ccgcagggta gctggcaggg cgcccaggcg
    12121 gcggttgtca gggctttggt tgaacttgcc gtcggcgttg tagcgcaccg acgccttcac
    12181 gcaacgggcg ggcaaataca acaggtaatc cggcctccct gtccggttga aggcgtcccg
    12241 cactttgtcg taatactccc ggcggttctc ctgctgcgct cgccagagac tctcgtcctg
    12301 gcgggtcaac ttttccggcg attccacaat cgcccgccat aggtccacca ggggcttatt
    12361 caggtcgttg atgacatcgt gcgcggccaa gccacgcgcc gccgctgcca gggtaatagc
    12421 cgccgatccg gcgaacggct cgatccgcgt cgtcgcccgc tccgggaagt aacggagaat
    12481 ctccgtggcc aggttacgtt tgctgccttg gtaggggatg gggtgtggga ttctcaaatg
    12541 accctccgtt catgggttcg ccggatcggc catgtgactt tgattgcatg tagttagcgt
    12601 tcgctacctt ttgcatctgc tccacaaacc cggcgcgcgt tttcaaggac gtagttggcc
    12661 ctcacccacg ccgcgctgag tacgcgcgcc gcgtcaaaag tgatttcctg tctgtattgc
    12721 cataaacgaa ggtgttgacg cctcaatcgg ctcttgttag gggtggcaca tgcttggtga
    12781 gcccgaaaac cagttttccc tcacccgcac cgtgcccatt accggcgcgg tgccctcaag
    12841 caaacgctta tcaattggaa ggccgcgggc ggaaacttca gccgggcggc catccttccg
    12901 gcgcacacct ccgcaaacgc ccatgccccg gcgcggggcg tcgcgagcgt gacgatgagc
    12961 cgcccgccgc tggcgcaagc atcgggggta aaggagccgc gcccggaggg gggcctggtg
    13021 gcgctggcgg ttgcgccggg gcacgataag ctccgaaccg cgggcaacga atgatatgaa
    13081 tggtgttaaa caaccggttt tggttcccgt gattacggcg ggattggtgg gattcgcctg
    13141 cgtggccggc gcggccgagc gggggccaaa ggagcgccgc gcccaaaccc gcccgccgaa
    13201 tattgtgctg gtgctggccg accagtggcg ggcggaggcg tttggttatg cgggcaatcc
    13261 cgacgtgaag acacccaacc tggaccggtt gcagcgcgag ggcatccact ttgtcaacgc
    13321 cgtgtccggc atgccggttt gctgcccggc gcgcgcgtca ttcctgacgg gccagcgccc
    13381 actcacgcac ggggtcttcc tgaacgacgt gccgcttaac ccggaagcgg tgaccatcgc
    13441 caaggtgctg ggcgcggcgg gctacgacac cgcgtatatc ggcaagtggc atgtggatgc
    13501 gcatggccgc tcgagcttca tcccgcgcga acggcggcaa gggtttgatt actggaaggt
    13561 gctggagtgc tcgcacgact acaatcactc ggcctactac gccgacggcc cggaaatgct
    13621 ttactgggag ggctacgacg ccatcgccca gacgcgggac gcgcagcaat acctgcgcga
    13681 ccatgcgcca ccgcgaaagc ccttctttct cgtgctgtcc tgggggccgc cgcacgatcc
    13741 gtatttcacc gcgcccgaaa aataccgggc cttgtacgac cccgcgaagc tgaccctgcg
    13801 gcccaatgtg cccgaggccg aagccgccca ggcgcggaaa atattggccg gctactatgc
    13861 gcattgcacg gccctggacg attgcttcgg cgagttgcgc cgcaccctgc gcgagaccgg
    13921 gctggaggaa accacgttgc tggtgttcgg ctcggatcac ggggacatgc tgggatcgca
    13981 aggcttcttc aagaagcagc ggccctggga tgaatcaatc cgcgtcccgc tggtcctgcg
    14041 ctggcccggc ggcctgggca cccgggcgcg cctcctggag gcgcccttca acgtcgagga
    14101 cctgatgccc accctgctcg gcctgtgcgg cgtgccgatc ccgaaaacgg tggaggggct
    14161 ggattacagc ggctacctcc gcggccggaa aaacccgggc gacaacgcga cggtgatcct
    14221 ctgcgcctcg cccttcggag aatgggagcg gcgaaagggg ggccgggaat accgcggcat
    14281 ccgcaccccg cgccacactt acgtgcgcga cctgaacggc ccctggctcc tgtacgacaa
    14341 ccaggccgat ccctatcaga cgaacaacct ggcgaaccag gcgaagcatg ccagattgca
    14401 ggcccggctt gacgccgtgc tcacccgcaa actcaaggaa cggcacgacg cattcctgcc
    14461 cggcaatgcc tacatccagc agtggggcta tcaagtagat gccaacggca ccgtgcctta
    14521 caaacactga cctcccccac gcagcactgg gcacaggcgg gcgcgcaaaa aacgatttcc
    14581 gatcaatgcc aagccgtgac cggtaatacc aacgaggaag gcgttgcccg gtagagaagc
    14641 tgcagcaggc ggcgcttcgg caggcttccc aatgagtccg ccgccggccg cagcgcaata
    14701 atcaaaaccg caagcggcaa atatctgccc ggggcttttg aaccagcttg gccggctgat
    14761 ggcggttcag aacggttttg ctggcctttc cgggccggtg cggatagact cgcggctgca
    14821 ttgaacgcag cctgacgcgg gccccggtcg cgggggtgcc ggcaaaaacg attaggcgcg
    14881 aacgctggct attcgtcgaa gagatcgccg ggcttccggg cgtcaacccg ggcccttttt
    14941 gtcaggagag cgcacggcag aagatgcctg gaaaatcccg ggcgcctgcg agaggggcgc
    15001 ggcgatggct gcaggcgcgg atgggcacag cgcattttta acgcgaacgt tagggctggc
    15061 gcggacgccg ggagaagagc gatcgctaaa gggcgccccg gtggtgatgg atgaactgaa
    15121 gcaactgatt gcgaagagct gaattatgga agaacgaact ggagtggacg agaatgttct
    15181 ctttcctaaa ttcgagaagc ttaagacgga agaggttcgg ggggcgaggg acacgttcca
    15241 cggccggatg cggatcaaca ctcgggggca ttaccagaac tttgcggggg cgattctgga
    15301 ggtggggcag cacctgaaag cgcagggcta atatgagcgc ggcatcacga ggcggagagc
    15361 tgttcatcgt agataacagc gacacggact ggaaggggtt gcgctacctc caggactgga
    15421 cggagattgc cagcgcgttc gacattgcga ccgggttttt cgagattggc gcgctgctgg
    15481 cgctggatgg gaagtggcag aaactggaca ggattcgcat tctcatgggc ggccagacga
    15541 cggcgcggac gcggcaggcg atccttgagg gtctgcgccg gcacgtgcgc gagaagctgg
    15601 atggcagcct ggagcgggag aaagagagca acgacttcct tggcggtgcg cgggcgattg
    15661 tggaagccat gaaggccggg aagatccagt gccgggtgta tgcgaagcgg aaatttcacg
    15721 cgaaggcgta catcacccat ccgcgcgtgg ccgtcatcgg atcggtggcg ctggtggggt
    15781 cgagcaactt caccgtgccg ggtctgaccg agaatgtgga gttgaacatt cagatccggg
    15841 cgccggggga tgtggcgcgg ctgcaggaat ggtacgagca gcactggcag gaggcggagg
    15901 aggtcatgcc ggacgtgatc agggtgatcg agcggcacat agacgagtac ccgccgttcg
    15961 aggtgtatgc caaggcgttg caggaactgt tcgagcacca cacgccgacg gaggcggaat
    16021 gggaaaacgg ggcctcgaaa atgtacccgg tgctggacca ctaccagaag gagggctacc
    16081 actcgctgct taacatcgcg cggcagcaca atggggcgtt tctgtgcgac ggagtcggct
    16141 tgggcaagac gtttgttggc ctgatgttga ttgagcggtt ggtgatgcac gacaagaagc
    16201 gggtggtgct gctggtgccg aagtcgggcc gcgtggcggt gtgggagcgg agcatccggc
    16261 ggtacctgcc gcacctgctg aatgggttcc tgccgttcaa aatctacaac catacggacc
    16321 tgatgcgggg gccaagcggc gacgggcgcg actttccggc cgaattccag cagatcaagg
    16381 agcaggcgga cgtggtgctg atagacgagg cgcaccattt ccggaatcgc gggctggcga
    16441 atgccgagga cgggacgatc cggtcgcggt attggcagct ttacgacatc atcgggcaga
    16501 agaccgtgtt cctgctgacg gcgacgccag tcaacaacgc gctgacggat tttcagcatg
    16561 agatcgagct attcagccgg gtggacaacc caacggcgtt tgccggggcg ctggggatac
    16621 acaacctggc gagccatttc cagcagttgg aaaaggcgct gaagcggctg acagcgggcg
    16681 aggagcacgg ggaactgttt caggtgaacc aagcggaagc ggagaaggtg ctgtttgacg
    16741 acaagctctt tcgctcgctg gtggtgcagc ggagccgggc ctacgcgcag cagagccaac
    16801 tgcaacacgg aggcacacag gcgatgttcc cgaccaaaga gccgccgaaa gtccagccgt
    16861 attcggtgaa gaagacttac ggccatttgc tggggaaagt ggaagcggcc ttctccaagc
    16921 cggaccagct attcagcctg gcggtctatt acccgttggg gcactggatt ggcgacccga
    16981 gcgcactgag gtccttcgac gtgaaccggc agaagcaggt tgtccgcctg attcgcattc
    17041 aattcctgaa gcggttcgag agctcgattt acgcgtttga aacctcgtgc cagaacctgc
    17101 tgttgaagct gctggcgttc ctgcggaaaa actcgacgac gccaacggag gtgaagcggc
    17161 tgcaccgttg ggaggcgcag aatgccgagt tgctggagca cgtgaaagag cgccgctccg
    17221 aactccagga ggaggatgag agcgaggaag cggacacgtc ggagctgggg gatgagttct
    17281 tggatgactt tgaggaacta agccggacgg attacaacct gccggagatc tttgatgaga
    17341 cctacgcgga tctggaacaa ctggtggact tcctggagga actgaaaacc tttaatgcga
    17401 gccacgacaa caagctgcaa tcgctgatca agctgctgaa gggggatgcg gtactgaaga
    17461 agcaaaaggt gctgatcttt tcggagttca tggccacggc ccggtacctg cggcgggagc
    17521 tgctgaaggc cgggattgaa ggggtggagg agattgacag cgcgagcacg atcgatcgtg
    17581 gggacgcgat ccagcggttc gcgccatact ataatgggtc cagcagcggt gagctggcgg
    17641 ccaaaggcca caaggagacg cgcatcctga tttcgaccga tgtgctgtcg gaaggcctga
    17701 accttcagga cgccacccgg ctgatcaact atgacctgca ctggaacccg gtgcggctga
    17761 tgcaacggat tggccgtgtg gaccggcgcc ttaacccgga gattgaggcg aagatggtgg
    17821 cggaccatcc ggacctgaaa ccgctgcggg gcaaggtgat ctactggaac ttcctgccgc
    17881 cggaggacct ggacggcctg ctgcggctgt accaccgcgt atcgagcaag acgctgcgca
    17941 tttcgaaggt gtttggcatc gaaggccgga agctgctgaa gccggaagac gatttcgacg
    18001 cgctgaagga tttcacgcat aagtaccagg gggagctgac cccgctggag aagatgcacc
    18061 tggagtttca gaagctgctg gcggacaacc cggggttgca ggatcggttg aacggcctgc
    18121 ccggccgggt tttcagcggc agagagcatc cgcaggcggg ccccaaggcg gtgtttttct
    18181 gctacgcact gccggcggag gccaaggggc tggacgggag cggcgcggac aaaaccgaaa
    18241 gcgaaaagca gaacacggaa acgggcggcg acgcgccggc ctggacgacg gaggcgagga
    18301 aggcggcgtg gtacctttat gacctggccg gcgggaagat tctggagcgg gcggaggaga
    18361 tcgtggcctt cgtgcgcagc gggccggaga ccgcgcgccg ggtgatcctg ccggagacgg
    18421 agctgcggga cgcgcggttg agggtcgaga aacacatcaa gaacacctac ctgaaacagg
    18481 tccaggcccc ggtgggagtg aagccggttt tggcctgctg gatggagttg aattgaagcc
    18541 atgatgacct tacgacaatt tgcggctgaa ccggccaccc tgccgatgcg cgccacctgg
    18601 tatttgaccg atctggcaga ggccaaaggc aggcacgagc tctacacccg ccaatctccg
    18661 cagaaactga aagtcctgcg cgaacacgcg ctgatcgaga gcgcggtttc gtccaaccga
    18721 attgaaggcg tcgaggtgga tgcgtcgcgc gtcgccacgc tgatgttcgg caagcccgcg
    18781 cttcgcgacc gcgacgaaga ggaagtgcgc gggtatcgtg acgccctgag actcatccat
    18841 gagtcaagcg cgcggctcgc gatcagcgag gagaccatca agcgcctgca ccatctctgc
    18901 cgcggccaaa tttgggatgc tggcgcctac aaggagaagg acgtggacat cattcaaacc
    18961 tacgcggagg ggcggagccg ggtccgcttc aagaccgtcc cggcgagcca gaccccggcg
    19021 gcgatggctg agttagtgga gctttggcag cgggggatgg ccgaaacgtg ggcgcccccg
    19081 atcgtcttgg ccgctgccct taatctcgat ttcctctgcg ttcacccctt ccgcgacggc
    19141 aacgggcggg tctcgcgcct cctcttgctg ctggcgacgt atcactgcgg catcgaaatc
    19201 gggcgttaca tcagcctgga gcggctgatc gaggaaaaca aggaacgcta ctacgaagtg
    19261 ctcgaacaaa gttcccagcg ctggcatgag ggcaagcacg atccctggcc ggccatgaat
    19321 ttcttcttgt ttatccttgc ccaggccgcc agggaattcg agcagcgcct gggacagatc
    19381 aagaccccga aaggcgcaaa aaccgcgttg atcgaacacg ctattgatgc cctgggcgaa
    19441 tttggaattg cagacctgga gcggcgctgt ccggctgtca gccgcgacat gatccgccac
    19501 ctgctccgca cgctgaaagc cgccgggcgt gtcgagtgcc tgggccgcgg gccaggggcg
    19561 aaatggagga ataagggtaa taccccaaaa ggaggcaaga ataagggtaa taaccagggc
    19621 aattaggtaa taaccaacga gctgcacccc ggaaccccat gcccaaatac gccgccaacc
    19681 ccgagccaat ccaaaggata agaacgctgc ccgaccacat cgttgaagca gatgaaggcc
    19741 gcgggggtga taacgtgttt ggggcgcggg cccggcgcga agtggcggaa gggaggaaag
    19801 agggtcatac cggaaaatga gggtcataaa gagggtcata agcgaacggc ggaagcgaga
    19861 aaacggtgag aaccatttat gtccaaccaa cttgatctgg agcgcctgcg gtcgctaaaa
    19921 accctgccgc aactaatcgc ctacctgcgg gacgaactgg actggccgat tgaccagaac
    19981 gcggtggcgg atgactacat cttcgaatac cgcccggagg agctgggctt tgatgcggac
    20041 gccgcggtcc gcatcaggga gatcaagcaa ctgcggccgc tcgaaaaggg gcagccgtgg
    20101 ggcatcttct gggtgagctt cgcgaagaag cggctgccgg tggtgatgct gcggcgcatt
    20161 cttggccacc tggtgctgaa gaagcgggcg gcggcgcaaa aggccagcca gcgggcctgg
    20221 catctgcatg acttgttgtt catctcggcc tatggcgagg atagcgaccg ggcggtctcg
    20281 ttcgcgcact tctcgcagga gccggaaagc ccgggcgacc tgccggtcct gaaagtcctt
    20341 gggtgggatg acggggacac cgtgctgcat ctggcggacg cccaccgcac gctggcggaa
    20401 aagctgcgtt ggccgggcaa gccggtcaac ctgaaagcct ggcgcgagca gtggtcttcc
    20461 gcgttcacgc tccgcccccg cgaggtcatc accacgacga cccagctcgt cgaggaactg
    20521 gcccggctgg cggaggccac ccggcggcgg gccaactcca tcctggcgcg cgagtcagag
    20581 cacgggccgc tccggaagct gtgcgcggcg tttcgcgccg ccctcattca tgatctgacg
    20641 gaggatgact ttgcggatgt catcgcgcag accatctcct acgggctgct gatcgcgcgg
    20701 ttctcccggg cggcgatttc ggcgcaaaac ctggtggata tggtgccgca aaccaacccc
    20761 ttcctccggg aactgctggc cgcgttcctc gcgctggcag gccggcagaa agctttcgac
    20821 ttcgacgagc tgggcatcca ggacgtggtc gagctgctga accaggccaa tgcggacgcc
    20881 gtcaaagctg acttcggcaa ccgtacccgc aatgaagacc cggtcatcca cttctacgaa
    20941 cacttcctgg cggcctacga ccggcgaaag aagattcagc gcggcgtctt ctacacgccg
    21001 cagccggtgg tgtcctacat cgtgcgcagc gtccacgaac tcctgcaaaa ggaattcgac
    21061 atcgaggatg ggctggcttc cacggtgacg tgggcggaca tgctcaagcg gtggccggat
    21121 ctgaggctgc cgaagatccg ggtgcccaag gtgggccagg cggagggggt ggaggtggcc
    21181 ctggcggcgg acgagttctt tgtgatgatc ctcgacatcg cgactggcac cgcgacgttc
    21241 ctggtcgaaa ccatcgaagt catcttccag cacctcaaag cgaaatgg
//
LOCUS       DAOPQG010000061        21194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3090_length_21194_cov_15.312550, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000061 DAOPQG010000000
VERSION     DAOPQG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 21194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21194
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3090_length_21194_cov_15.312550"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1428)
                     /locus_tag="PKI07_08390"
     CDS             complement(64..1428)
                     /locus_tag="PKI07_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51108.1"
                     /translation="MVGLALLVGLAPFPLRAQTNSVQPKGNRYLFIVDTSRSMQRRSE
                     AMLQVVRGLILSGFNGQIRRGDTLGLWTFNQGLYAGRLPLQVWSHEGREEIAARTIAF
                     LETQQYAGRANFDSVQPALDRVIGNSDQITIMLISSGDEKVQGTPFDPQIADYYRRGR
                     NQQKKAQMPFVTILRAQLGQLIHCSLNMAPQALQFPPLPEAPPEVVAGKPSDPKRPAE
                     AKSSASSDQPKKQPRPEEAPVAKPAPVPVKAEAPVPSAMSTNDAKATAIKSSATSAIP
                     AKVEQAKAAPAEAKTPAPVPLLPKAEPPAPIIKPKIAPELVEPKVGASSPVASSAPKS
                     EADAPPKPVPAPESKPTPPPPPLVRESAGTSSLATATNIPFSSSPSVPAIVSSPAGAV
                     SVVQTAAVAAATPSASRKNLWLAALALALITGGIVALHYLQRSRRAPQGSLITHSIER
                     RKKP"
     gene            complement(1536..2006)
                     /locus_tag="PKI07_08395"
     CDS             complement(1536..2006)
                     /locus_tag="PKI07_08395"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOC51109.1"
                     /translation="MALVRILVDGYSLLYNWPHLVPGRAPHFAAARDALIGCLTQYQD
                     ATGIPVTIFFDGADSFAGRPIASSSSMVEVLYSRKGQTADQMIERAVHRFEPYGEVLV
                     VTEDRAERDLVVSLGGSVASCHSFIQDVENALADLGDEIKYHNRRERQRFQRPR"
     gene            2322..3893
                     /locus_tag="PKI07_08400"
     CDS             2322..3893
                     /locus_tag="PKI07_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51110.1"
                     /translation="MGQRIFVLSREPALRELLGAVASDGEVKYWGGIATLLMMVGWAT
                     GGILFGMMSDRYGRVKAMVSTLLAYTIFSGLSGFSRSGIEFLIYRFLFGLGVGGMFGA
                     ATTLVAESVPKHFRTMALGSMQALSAFGNMLASGLSLKITPGMENFWGHYSGWQVLSF
                     VSVFPALLAVPMVLLLKEPQVWIKAKAEAAGGGTAKRVGSFADLFQHPRWRKSTLVGI
                     CLGVAGMVGLWGIAFFSPELITTAFKNRPLQIEEILKPAEVCAALKSPPSPAVIHLKA
                     KLSPEVVRLVEDCGSEKDIPAATVQALVTDLNRLIQEDDLHDETAFQSVELKKRTRNL
                     IQRVQKNGERQNIISLNRQLVEHLFPSAIRELQRTIDRTISRGTLLQDVGALLGMFAF
                     TFAATYFNRRTAFLAAFVLCLCSVSYVFYALKTATDVLWMLPLIGFATLSCFAGYSIY
                     FPEIFPTRLRGTGVGFCYNTVRYLAAPFPFLLGWLSTLMPFRSVAMIMSSIYLVGIVA
                     LIWAPETKGQPLPED"
     gene            complement(4223..5653)
                     /locus_tag="PKI07_08405"
     CDS             complement(4223..5653)
                     /locus_tag="PKI07_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOC51111.1"
                     /translation="MHQRASLILTTILLLVAPLLALATDKKPFRAGVATSNITPWLDD
                     GLVGNFGTPPPAKYVHDELHARCFVLDDGATQIAIVVCDNIYISREVLDEAKRQITVA
                     TRLPADRILVSGTHTHSSVSARWKNPLRPEAEFSEYQRFIAHRIADGVRCALYNLQPA
                     KIAWGAVDLPGQVFCRRWLLKPGAEIFNPFGEPDQAKMNPGNRSDLLKPASPVDPQIV
                     FFALQTPAGQPIGLLANYSLHYVGGTGPNHISADYFAAFADRIQELLGADRLDPPFVA
                     AMSNGTSGNINNINFAVVREKRKPYEQIRLVADDCAQAVLKRYRELEWHEQASLGMRQ
                     RELELGVRKPTPAQLEHARKVLAGPQRADKFPHERNYAAKAIQQLESPDTVRVILQVL
                     RIGELGITAIPFEVFSDTGLELKARSPFKPTFTIELANGGYGYLPTPEQHKLGGYETW
                     LGSNKVELQASVKIVNTLLEMFSELK"
     gene            complement(5894..7156)
                     /locus_tag="PKI07_08410"
     CDS             complement(5894..7156)
                     /locus_tag="PKI07_08410"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOC51112.1"
                     /translation="MAYLAATAGADTQTSTSLQLVNYSLSAAAPGWYNAIYLQPKGGR
                     LRSGDRMSDNNGRTWQSKPMMPDFVTGLPHGYRREPITSVVDGFTGRLLTIVNALDTP
                     GLDPKINEPPVAQQTYYLRYRVSEGGGRSWLFDEPVVQVGGFTAQHPFEGVWIGKNAI
                     YLGDLGCIPIVTRTGRVLLPVQTTPLGPDGQLWNPNGGHSYTDVLVLRGTWTNGARLA
                     WRATQRLVGNPNRTPRGLIEPTLAEFSDGRILMVMRGSNGGQADPHYKLPSYKWFAFS
                     SDGGETWSAPEPWGFEDGSTFFSPSSMSALFRHSSGRCFWVGNLAESNCQGNLPRWPL
                     VVGEVDSRTLKLKRSSLLAVDAPTSADQSRGRLDLSHCTLIEDRKTKQIVLTYPRSYN
                     AYQSNEWATVRLAVRPAKPRLLPSPKSR"
     gene            7709..8782
                     /gene="recA"
                     /locus_tag="PKI07_08415"
     CDS             7709..8782
                     /gene="recA"
                     /locus_tag="PKI07_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOC51113.1"
                     /translation="MPPKTAEKFVAADTRATEAAKASAARQRDLDAAISAITRAYGDG
                     SIMRLGDARALRRIEVIPTGALALDLALGVGGIPRGRVVEIFGPESSGKTTLMLHVIA
                     NAQKGGGLAAFIDAEHALDPAYAKRLGVDLDDLLVSQPDSGEEALSICETLARTNALD
                     VIVVDSVAALVPKAELEGEMGMATMGMQARLMSQALRKLTAILAKAKTTCVFTNQMRE
                     KVGVMFGNPETTPGGKALKFYASVRIDIRRKDTLKDAAGNAIGNHVKVKIVKNKVAPP
                     FAEAEFDILYNHGINKEGSLLDVGIESGAVDKKGAWLQFEGELIGQGKDAAQRALAEK
                     PELAARITDAIMARRNAAPAEPK"
     gene            8910..9317
                     /locus_tag="PKI07_08420"
     CDS             8910..9317
                     /locus_tag="PKI07_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC51114.1"
                     /translation="MNLARKLLHTRYRVNDLERTVKFYREVLGLQEVRRHQSPRGAEL
                     VFLKAPDSEELIELCCFPASGPVQVQPDLTHLAFAVDSLEEFGRHLARLGLAYSDGPH
                     LNADGGGLAFIDAPEGYEIELIQPNRSGAEGGL"
     gene            9380..10165
                     /gene="folP"
                     /locus_tag="PKI07_08425"
     CDS             9380..10165
                     /gene="folP"
                     /locus_tag="PKI07_08425"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOC51115.1"
                     /translation="MGIVNVTPDSFYDGGRFLDTRAAVAHALQLVEEGAEIIDVGGES
                     TRPGAAPVTESEELRRVMPVIEQLAGRLKVPLSIDTAKPGVAQAALAAGASIINDVAA
                     YGEREAMWRLAAETRAGYVCMHAQGTPQTMQLNPAYTDVVREVGEFFAERVQRLRNCG
                     VFGEQLILDPGIGFGKTAGHNLQLLGAMRAFAKFERPLLVGVSRKSFIGTLLGAEPAE
                     RLPGALACACLTVADGVQLIRAHDVAETVQAIRMTEAILAKQN"
     gene            10176..11048
                     /gene="cdaA"
                     /locus_tag="PKI07_08430"
     CDS             10176..11048
                     /gene="cdaA"
                     /locus_tag="PKI07_08430"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOC51116.1"
                     /translation="MWQTLQTAWRPALEILILAAGIYGGFRFVRGTRGAPVVTGFLVA
                     LLALVLVSYLLKFKVLQYFLGGFSAFFVLAVLVIFQPELRRMLAELGNLPLFATAHEQ
                     RENIEVIIQTVERLADVRIGALIAVERSIHLQEAVESGIRVDCEATPEMLETIFFPNN
                     AIHDGGVILQGDRIAFAACIFPLTQRQDLHPSLGTRHRAAIGLSEETDAVVVAVSEET
                     GAISYAYKGQLMRGVTVEELRAFLTSVLVSPVRSRNWLGGPWFRNADDAKPGPVHVAR
                     ADSPAPSTGKSASL"
     gene            11045..11446
                     /locus_tag="PKI07_08435"
     CDS             11045..11446
                     /locus_tag="PKI07_08435"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOC51117.1"
                     /translation="MIAFLRNLVFEDFWLKLFSLVLAVLVWFIVTFASQTDAQTDERV
                     FSHLPIAVVSATEDVRDFRVSPNQVQVRVRGDSKTLQNVQSKDIRAIVDLTGVTTARE
                     LRKRVEVAAPTGITHLRVVPEEVQVIFPPDR"
     gene            11829..12554
                     /locus_tag="PKI07_08440"
     CDS             11829..12554
                     /locus_tag="PKI07_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC51118.1"
                     /translation="MSPLPSPDSPLSELPPSLPASNRKISLLLADDHPVVRRGIKSCL
                     TPCPRIEVLGEAADGQEALSKTRELMPDVLLTDLEMPNLSGLAVAEALRKEHSKTRVL
                     ILSAHANPTYVLRSARAGASGYVLKQAPPDELVRAIETVNAGQPFFSPEALHATLNQY
                     VRGSGQEFHPAGLTTREREVLVYIAEGLSNKEMAERLHISPRTVETYRERLMRKLDIH
                     TIAGLTRFAVAQGLTTIPELAVV"
     gene            complement(12614..13393)
                     /locus_tag="PKI07_08445"
     CDS             complement(12614..13393)
                     /locus_tag="PKI07_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51119.1"
                     /translation="MSRAGARVRREPGRAFTLIELLSVIAVIAILAALLLPALAHGRA
                     SANRVKCASGLRQLGLAAQLYWDDNAGNCFRYLMGQTNVGQLCWFGWLGPGEEGQRPF
                     DATRGALYPYLRGRGVEICPALDYSLARFKLKASGAAYGYGYNLALSVSLKKPPVRIA
                     QITRPADQALLGDAAQVNDFQAPASPANPRLEEFYYLDAPTNYASRAYYPNGHFRHAH
                     KANVVFCDGHVAGEGMVPGSLDRRLPDLHVGSLRPEILRLP"
     gene            complement(13390..14385)
                     /locus_tag="PKI07_08450"
     CDS             complement(13390..14385)
                     /locus_tag="PKI07_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51120.1"
                     /translation="MWKARWFVLALASGLVVGAVQAQTLSMQEDFAADPAARGWLVHG
                     HTNLFYWNPTNQNLEVTWDSSLSNSYFCHPLGATLTMDDDFSVAFDLELHEAAIGGYG
                     FELALGMLNLADATQPGFLRGTGADSPNLVEFDYFPDPEGELWWGASVTTMMVDWVGT
                     DSSHWSLGGFAGMELAAHNLYHVELVYAADTRTLRTTITNLTLGGIAVGPVPEAYLPP
                     TFLGFRVDHIAIASYSDANSWGSLWARGTVDSLGVAGTARAIGRVQGGWGAGGVWQAR
                     FFSRADWRYTLERTTDFQSWTPVSATRQGVAGDLVLQDAEPPAAAAFYRVRAEQP"
     regulatory      complement(14649..14771)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            14896..15816
                     /gene="htpX"
                     /locus_tag="PKI07_08455"
     CDS             14896..15816
                     /gene="htpX"
                     /locus_tag="PKI07_08455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOC51121.1"
                     /translation="MKFAQISKRILLLVVVNLLVVTTISVVLSLLRVGRYFPEGGLTQ
                     LAIICLLWGFAGAFISLALSRLMAKWTMGVQVIPPNTSDPALRELVQTVHGLARSANL
                     PQLPEVGIYSSPEVNAFATGPTKSRSLVAVSSGLLQRMHPREVEGVLGHEIAHIANGD
                     MVTMTLIQGVINAFVLFLSRVLAFVISQALRSRDDRGGGGLLQFFLIMVFQFVFSFLG
                     MLVVCAFSRWREFRADAGGARLAGRENMIDALRALQRLHNPELAAAEARHAQSFQALK
                     ISGSRSRFMALLATHPPLEQRIARLAQGAA"
     gene            16028..17476
                     /locus_tag="PKI07_08460"
     CDS             16028..17476
                     /locus_tag="PKI07_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOC51122.1"
                     /translation="MIEFNNRILMTGFGVVAQAVLPMLLKHVRVPCRNITVIDFADCE
                     AELRPWIKRGARFIRERVTPTNLTRLLSTHVGRGDLIIDLAWGIEFFEILEWAHNNQV
                     LYVNASLESWHSSVEMPSKSSMAKSLYANYERLLPIVARWGPATTAVIDHGSNPGLVS
                     HFAKQGLLDIGEKTLRENHLPRAQTRRLERLMQEQSFAELARTLGVKVIHCSEWDTQR
                     ASKPKRPDEFVGTWSVQGMWDESISPSELGWGTHEKWLPPFATRPATGPANQIILPQM
                     GLNTWVRSWVPHQEIVGMIVTHGESFGLSHALTVREAGQAIYRPTVHYAYMPCNDSIV
                     SLHELRCRNYELHPRTRILRDEITEGMDLVGALIMGHRFQSWWTGSKLSIAAARRKVR
                     HSNATAVQVAAGVLAGVLWAIRNPQRGVCFPEDLPHEEILRDARPYLGRLVSEPSDWT
                     PLRRHRVYFDENPMAQGDQSDPWQFTNFLFRP"
     gene            17473..18747
                     /locus_tag="PKI07_08465"
     CDS             17473..18747
                     /locus_tag="PKI07_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51123.1"
                     /translation="MTPATRRSGAMLAALLLLGAAGAAAAGPVRFVQDRFVVGLWVPP
                     ATQENLDARYREIAEANFTLVVGQSGTNTAAHLRRCKRFGLKTLIPAHGPAEKLPDGP
                     ACWGYLLRDEPGAGAFAELARRAEEIRARRPGRFGYVNLFPNYAPPGALGTPTYEEHV
                     TRFVREVKPEVLSMDHYPLMRPEGDTRDQYCANLECFRRHSLAAGIPFWNYFYSMPFN
                     DRLDPTEAQIRWQIFTAAAYGARGVLYFCYWTPGLGAAGAGEFPKGGALLTAEGRKTR
                     HYEEARRINAELKNLGPTLMLLTSTGVYRVTTETNAPALAGSPLRQLARVGGDPIGHF
                     IVGAFAHADGRRAVLIVNHDYRYTAWPTVWFDAAAEDVWEVSKTTGRAAPVVDDSPEL
                     KGLQLSFGAGDARLFLLPPAERTPLRSPRGRE"
     gene            complement(18749..19030)
                     /gene="rplW"
                     /locus_tag="PKI07_08470"
     CDS             complement(18749..19030)
                     /gene="rplW"
                     /locus_tag="PKI07_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOC51124.1"
                     /translation="MNSFEIIRTVRLTEKGTRQGEKYNQYTVMADPRANKMQIRAAVQ
                     ELFKVKVTRVNTLNVRGKLRRQRTKQAGRSLNWKKAIVTLKEGDKIVLT"
     gene            complement(19032..19658)
                     /gene="rplD"
                     /locus_tag="PKI07_08475"
     CDS             complement(19032..19658)
                     /gene="rplD"
                     /locus_tag="PKI07_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOC51125.1"
                     /translation="MKLTIKDIKGNNQGELEVKFALIEDGKGTQAVHDAVVAYQAAQR
                     MGTACTKNVGEVAGTNKKPWRQKGTGRARAGSFRSPLWSGGGVVFGPKPRDFTKKVSH
                     RTRKLALRKALSARLRAGDVVVVDELKLGSGKTREFVGVLNALALKGTALIVAQAPDK
                     NLTLASRNVPNVALATGDSLSTYDVLRPDKLVFTRNAFEQVEARLGKE"
     gene            complement(19668..20363)
                     /gene="rplC"
                     /locus_tag="PKI07_08480"
     CDS             complement(19668..20363)
                     /gene="rplC"
                     /locus_tag="PKI07_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOC51126.1"
                     /translation="MPLGLLGKKLGQTRVYDARGMIAPVTIVQVGPNRVLQCKTQQSD
                     GYNAVQLGFDDQKEHRVSKALLGHLKKFNGGPVKRIREFRDFTKDVKPGDVVGADLFA
                     PGDYVDAIGLTKGRGFEGVVARYRFGGGPNTHGTKGWHRRLGAIGCRLFPGTVMRGTK
                     MPGHMGQVRRTVQNLEVIQVHKEDNVLLIRGAIPGANGDYVVIREAKKRPKGWKPHAE
                     REENLKKSAAKKK"
     gene            complement(20377..20685)
                     /gene="rpsJ"
                     /locus_tag="PKI07_08485"
     CDS             complement(20377..20685)
                     /gene="rpsJ"
                     /locus_tag="PKI07_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOC51127.1"
                     /translation="MTAQRIRIRLKAYDHRVIDQSAREITDTVKRTGARVGGPIPLPT
                     RIERFTVQRSPHADKKSQETFEQRTHKRLIDIIEPTARTVDELKKLNLPAGVDITIKI
                     "
BASE COUNT         4118 a         6695 c         6209 g         4172 t
ORIGIN      
        1 aagtcatgca cgccacgctc cctgcctcgc cgcaccctca atcgctgccc tcttaaaacc
       61 ggactacggt ttctttcgac gctcaatcga atgcgttatc agactgcctt gcggcgcgcg
      121 gcgcgagcgc tgcaaataat gcaaggcgac aatgccaccc gtgattagcg ctagcgcgag
      181 cgccgccagc cacagattct tgcgtgacgc cgaaggggtc gccgccgcaa ccgcagccgt
      241 ctgcaccacg gaaaccgccc ccgcaggaga cgaaacaatc gccggaacag atggcgatga
      301 tgaaaaaggg atattcgtag cggtggccaa agaactggtc cctgccgatt cccgcacaag
      361 cggtggcggg ggcggagttg gctttgactc gggagcagga accggcttgg ggggcgcatc
      421 tgcttcagac tttggggcag aactcgcaac tggcgatgag gcgcccactt ttggttccac
      481 cagttccggg gcgattttcg gttttataat cggcgctggc ggctccgcct ttggcaacag
      541 cggcacgggc gcgggagttt tagcctctgc cggagccgcc ttcgcttgtt cgactttcgc
      601 cggaatggcg gaggttgcgg atgatttaat agccgttgcc ttcgcgtcgt tcgtggacat
      661 cgccgaagga acaggcgctt cggccttgac gggcacgggc gcaggcttgg ctacgggtgc
      721 ctcctcaggg cgaggttgtt tcttgggctg gtcagatgat gccgaagatt ttgcctccgc
      781 cgggcgtttg ggatcagatg gcttgcccgc aacgacctcc ggcggagctt ctggcaaagg
      841 aggaaattgc aacgcctggg gcgccatgtt aagggaacaa tggattaatt ggcctagctg
      901 cgccctcaaa atcgtcacaa acggcatctg cgcttttttt tgctggttgc gcccgcggcg
      961 ataataatct gcgatttgag ggtcgaaggg agtgccctgg actttctcgt caccgctgga
     1021 aataagcatt atggtaatct gatcggaatt cccgatgacg cggtccagcg ctggctggac
     1081 gctatcaaag ttcgcccgcc ccgcatattg ctgagtctca agaaaagcaa tcgtgcgcgc
     1141 ggcaatctcc tcccgtcctt catgcgacca cacctgcagg gggaggcgcc ctgcatacag
     1201 cccctggtta aaggtccaaa gacccagcgt atcgccacgc cgtatctgcc cgttgaaccc
     1261 ggacaggatc aaaccgcgga ctacctggag catggcctcg ctgcgccgct gcatggaacg
     1321 ggaagtgtcc acaataaaca ggtagcggtt gccctttggc tgcacactat tggtctgtgc
     1381 gcggaggggg aacggagcca gccccaccaa aagcgccagc cccaccattc ccagccgaat
     1441 caaccaacac ccggaaccgc gaccgtaacg ggattctcca actcttttat caatctggtt
     1501 ttgttttccc acgctactct ttctggaaca gccagctacc tcggccgctg aaacctttgc
     1561 cgctctcggc ggttgtgata tttaatctcg tctcccaagt ccgccaaggc attttcgacg
     1621 tcctggatga aactgtggca actggcgaca ctgccgccga ggctgactac cagatcacgc
     1681 tcggcgcggt cttcggtcac gaccagaacc tcgccatagg gctcaaaacg atgcactgcg
     1741 cgctcaatca tctgatcggc tgtttgtccc ttgcgagaat agaggacttc aaccatggat
     1801 gacgaagaag caattggcct gccagcaaac gagtccgcgc cgtcaaaaaa gatcgttacg
     1861 gggatcccgg tggcatcctg atactgggta agacatccaa tcaatgcatc ccgcgccgcg
     1921 gcaaaatgtg gcgctcgccc cggaaccaaa tgaggccagt tatacagtaa gctataacca
     1981 tccaccagaa tccgcaccag cgccatgcca gaccataccg ctcaacctgc tgtttgaaca
     2041 atagaaactt ggcggggcgg gagaagttct cgtcgcctgg cactcaaatc tgatattagc
     2101 cgcagcaaca cgaaaagcgc gcagaagcgc ccgcagcgct tgacaggtcc gcccgtaatt
     2161 tagcaggata ggtcgcgatg cgcccttcgt ccaactctgg aagtccggct cacccgcggc
     2221 cggttagttc cgcagtaccc tcggaagcga ttgttgaacc catcacctgg tatcactggc
     2281 tggtggtctt ttttgcctcc tgcggatggc tttttgactg catgggccag cgaatctttg
     2341 tgctgtcgcg cgagcctgcg ctgcgggaat tgctcggggc ggtggcttcg gacggcgagg
     2401 tcaaatactg gggagggata gccactctgc tgatgatggt tggctgggct acgggcggca
     2461 tactgtttgg aatgatgagc gatcgttatg gccgggtaaa agctatggtc agcacgcttc
     2521 tcgcttacac catcttctcg ggactttccg ggttttcccg gagtggaatt gagtttctga
     2581 tctatcggtt tctattcgga ctgggagtcg gcggcatgtt tggcgcagca acgacgctgg
     2641 tggcggaaag cgtgcccaag cactttcgca cgatggcgtt aggctcaatg caggctctat
     2701 cggcgttcgg caacatgctc gcctccgggc ttagcctgaa gattacccca ggcatggaaa
     2761 acttctgggg ccattatagc ggttggcagg tgttgtcttt tgtcagtgtg tttcccgccc
     2821 tgctggcagt gcccatggtt ctgctgctga aagaaccgca ggtttggatt aaggccaagg
     2881 cagaagccgc gggcggagga accgccaaac gtgttggctc gtttgccgat ttatttcaac
     2941 acccgcgttg gcgcaaaagc actctggtcg gtatttgcct aggcgttgcc ggcatggtgg
     3001 gcctgtgggg aattgctttc ttttcgccgg aactgatcac caccgccttc aagaatcggc
     3061 ctttgcagat cgaggaaatc ctcaagccgg cagaggtttg tgcggcgttg aaatcgcccc
     3121 cctcccctgc cgtcatccat ctgaaagcca agctgtctcc ggaggtggtc cggttggtgg
     3181 aagactgcgg aagcgaaaag gatatccccg ccgcaaccgt gcaggcgcta gtaaccgacc
     3241 tcaaccgtct cattcaggaa gacgatcttc atgacgagac ggcctttcaa tcagtggaat
     3301 tgaaaaagcg cacccggaat ctaatccagc gcgttcagaa aaatggggag cgacagaata
     3361 tcatttcact caatcggcaa ttagtggagc accttttccc cagcgccatt cgcgaactgc
     3421 agcggaccat tgaccggacc ataagccgcg gcacgctgtt gcaggacgtc ggcgcgctgc
     3481 tgggcatgtt cgcctttacc ttcgcagcga cttatttcaa ccggcgaacg gctttcctcg
     3541 cggcctttgt cctttgtttg tgctccgtgt cgtatgtttt ctacgcttta aagacggcga
     3601 ccgacgtgct ctggatgctg ccgctgatcg gatttgcaac cttatcttgt tttgccggct
     3661 actcgattta ttttcctgaa atattcccca cccgcctgcg gggcactggc gtcggcttct
     3721 gctataacac cgtccgttac ctggccgcgc catttccatt tctgcttggc tggttgagca
     3781 ccctgatgcc gttccgctcg gtggccatga tcatgtcttc gatctacctc gtcggcattg
     3841 tcgcattgat ttgggccccg gaaaccaagg gccagccatt gccggaagat taagggggaa
     3901 tcagaagaaa acccccacac ggatttgacg atcggacgaa gagaattaat ctgtcgactc
     3961 cgttggcgct ttactcgcca acaacatcgg cagtgccgac tgggaagagt ttcggggcca
     4021 tcgactgcta aaatacggat gagcagcagc aaccggatgg ccgcgagcgg acaggagatg
     4081 cccatttatc ggccaaggga taaccagcgg aagcaacctt cgctgaccgc ctacaacgat
     4141 tgctgctacc gtggcgaagc gtcaaccttg cagttcctcc cttttttcaa gtggaacccg
     4201 gagcttatgc tccaattctg ttctatttca actcgctgaa catttccagc agcgtgttga
     4261 cgatcttgac cgatgcctgc aactccactt tgttcgagcc cagccacgtt tcgtaacctc
     4321 ccaatttgtg ttgttcggga gtgggtagat agccatatcc accattggcc aattcgatgg
     4381 taaaagtggg tttaaacggg ctgcgggcct tcaactccaa gccggtatcc gaaaacacct
     4441 cgaacggaat cgccgtaatg cctagctcac caatacgcaa gacttgaagg atcacgcgga
     4501 cggtatcggg cgattcgagt tgctggatcg ctttggcggc atagttccgc tcatggggaa
     4561 atttgtctgc acgctgcgga ccggccaaga ccttgcgcgc atgctcaagt tgggcgggcg
     4621 taggtttgcg cactccgagt tccagctccc gttgccgcat ccccaatgat gcctgctcgt
     4681 gccactccag ttcccggtac cgcttaagaa cagcctgggc acagtcgtcg gcgaccaaac
     4741 gtatttgttc gtagggtttg cgtttctcgc gcacaaccgc gaaattgatg ttgttgatat
     4801 tgccgctcgt gccattgctc atggccgcca caaagggcgg atcaagccgg tcggcaccca
     4861 acaattcctg aattcggtca gcaaacgcag caaagtagtc ggccgagata tggttcggtc
     4921 ccgtcccgcc cacgtaatgg agtgagtaat tggccaatag tcctatcggt tggcctgcgg
     4981 gcgtttgcag agcaaagaat acgatctggg gatcaaccgg gctggccggt ttcaacaaat
     5041 cggaccggtt gcccgggttc attttggcct ggtcgggttc gccaaagggg ttgaatattt
     5101 cggcacccgg cttcaacagc cagcgccgac agaacacctg gccaggcaga tcgaccgcac
     5161 cccatgcgat cttcgccggc tgcaggttgt agagcgcaca gcggacgcca tcggcaatgc
     5221 ggtgagcgat gaatcgctgg tattcggaaa attccgcctc gggccggagt ggattcttcc
     5281 agcgggcact gacggatgaa tgcgtatgcg tgcctgatac gagtatccga tctgctggca
     5341 gtctggtggc cactgttatc tgccgcttgg cttcatcgag cacctcgcga ctgatgtaga
     5401 tattgtcgca aacgacaatg gcgatttgcg tagcaccatc gtcgagcaca aaacagcgtg
     5461 cgtgcaattc gtcgtgaacg tatttagctg gaggcggggt cccaaaattg cctactaatc
     5521 catcgtccaa ccagggggtg atattactgg tggcgacacc ggcccggaag ggctttttat
     5581 cagtcgccag ggcaagaagc ggcgctacca gcaataaaat cgtcgttaga atcagagaag
     5641 cacgttgatg cataattctt tcctcaagtt gtttatatta aacggttttc cacgcgattc
     5701 tgacggtggc gaccggcgcc ggtggcactg ccccatcgta agtgaaccga accaattcgc
     5761 ttttggtgcg atgcgcccaa actcaacgca gcgcgggtga aacagcatgt tctttccgtc
     5821 tcaccaagcc tgtctcgata ctggcatagt tagcgaaaaa gtcaaccaca ccaacggatc
     5881 aattcacggc cttttacctc gatttcggac tggggagcag gcgtggtttc gccggcctta
     5941 ctgccagccg cacggtcgcc cattcgttgg actggtaagc gttgtaggag cgaggatagg
     6001 tgagaacgat ctgtttggtt ttccggtctt caatcagggt acagtgcgac aggtccagcc
     6061 gcccgcgaga ttggtcggct gaggttggtg catccacggc gaggaggctg gaacgcttca
     6121 acttcagggt gcgagaatcc acctccccga ccaccagcgg ccagcgtggt aagttaccct
     6181 gacaattaga ttcagccagg ttgcccaccc aaaagcaacg tccgctggaa tgccggaaca
     6241 atgcggacat cgaggagggt gagaaaaagg ttgacccgtc ctcgaacccc caaggttcag
     6301 gtgcagacca ggtttcacca ccgtcagacg agaacgcaaa ccatttatag ctgggcagtt
     6361 tgtagtgcgg gtccgcctga ccgccattgc tgccgcgcat aaccattaga atgcggccat
     6421 cggagaactc cgccagcgtg ggttcgatca ggccgcgagg cgtgcgattg ggattcccca
     6481 ccagacgctg ggtagcccgc catgccaggc gcgcaccgtt ggtccaggtt ccccgcagca
     6541 ctagcacgtc agtgtaagag tgacctccgt tcggattcca taactggcca tccggtccca
     6601 gcggtgtggt ttgcaccggg agcagaaccc tgccagtgcg cgtgacaata ggaatgcagc
     6661 ccaggtctcc aagataaatc gcgttcttgc caatccacac tccttcaaag ggatgctgcg
     6721 ccgtgaaacc acccacctgc acgacaggct catcgaatag ccaactccgc ccgccgcctt
     6781 ccgacacgcg ataacgcagg taataggtct gctgcgctac tggcggctca ttgattttcg
     6841 gatccagtcc cggggtgtcg agcgcattga caatcgtcag cagccggccg gtaaaaccat
     6901 ctactaccga cgtaatgggc tcccgccgat agccatgagg caatccggtg acaaaatccg
     6961 gcatcatcgg tttgctttgc caagtgcgcc cattattgtc ggacatccga tcgcccgaac
     7021 gaagtcggcc gcccttgggc tgtaagtaaa tggcattata ccaacccggc gcggcggccg
     7081 aaaggctgta gttaaccaat tgtagtgacg tagacgtttg agtgtcagcc cctgcagtgg
     7141 ccgcaagata tgccataggc actacaagcg cgagcctcgg aacaaaccca cataatcttt
     7201 tcatggaaaa ctgcattgct ttgcgcattc gcccgtactg tgcatcgcac ggatgaggga
     7261 gatttggttt tgcccctcgt cgagcatggc acaacattag aaagagcaac ctggagcgtc
     7321 aagcgctttg cttatgcggt ctccgttgtt ccctccgctt tttctttgtc tcaaaacggc
     7381 agcccctcag atgcagatta cgattagata gagagggaag agggcgaaga aatgcggcta
     7441 aagaaattgg aaggtcaaat taaacccaac gctatgcgta acagggtatt tgttctgcct
     7501 ggcagttctg gggaacatcc ttaccggacc gcaactacct tgccgctgat gacaggctac
     7561 aggtgagatt ctcggcattg gccggacatt gagctccgca agccagaaaa cagcgaaggg
     7621 tctgaaaaca ggcgtttgac tactcgcgcg gaatgtccca atatagccaa cgattgtccg
     7681 taagatttac gattacgtat acacaaatat gcctcccaaa actgctgaaa aatttgtggc
     7741 cgccgatacc agggcgaccg aagctgccaa ggcatccgcc gcgcgccagc gcgatctgga
     7801 tgccgccatt tccgccatta cccgagccta tggtgacggg agcatcatgc gcctgggtga
     7861 cgcgcgagcg ctccggcgaa tcgaggtcat ccccaccggc gccctggcgc tggacctggc
     7921 gctgggcgtg ggcggcatac cgcgcgggcg cgtggtggaa atctttggcc ccgagtcttc
     7981 gggcaaaacg acccttatgc tgcacgtgat cgctaatgcc cagaaagggg gcggactggc
     8041 ggcattcatt gacgccgagc acgcgctgga tccggcctat gccaagagac tgggagtcga
     8101 cctggatgac ttgctggtat ctcagcctga cagtggcgaa gaagcgctgt ccatctgcga
     8161 gacgctggcc cgcaccaacg cattggatgt gatcgtcgtg gactccgtgg cggccttggt
     8221 gccgaaggcg gaattggagg gcgagatggg catggcgacg atgggcatgc aggcacggtt
     8281 gatgagccag gcgctgcgca aactcaccgc catcctggcc aaagccaaga ctacctgcgt
     8341 cttcaccaac caaatgcgcg agaaggtcgg cgtgatgttc ggcaaccccg aaaccactcc
     8401 ggggggcaag gcgctcaagt tttacgccag cgtccgcatt gacatccgcc gcaaggatac
     8461 gctcaaggat gccgcgggta acgccatcgg caaccatgtg aaggtaaaga tcgtcaagaa
     8521 caaggttgcc ccgcctttcg ccgaagccga gttcgatatc ctctacaacc acggcattaa
     8581 caaggagggc agcctcctgg atgtcggcat cgagtcgggg gccgtagaca aaaagggcgc
     8641 ctggctgcaa ttcgagggcg agctgatcgg gcaaggcaaa gacgcggcgc agcgggcatt
     8701 ggcggagaag ccggaactgg ccgcccgcat cactgatgcg atcatggccc ggcgcaacgc
     8761 cgcccccgcc gaacccaagt aattcccacc aggccccgcc gtccgtccct cctgactccc
     8821 gcctgggaga acagataccg ggtggcgcat aaagcggggg tttaataatt gccgattgcc
     8881 ttgggccgat tccgcgttat cgtgggcgca tgaatctggc caggaaacta cttcatacac
     8941 gataccgcgt gaacgatctg gagcgaaccg tgaagttcta ccgcgaggtg ctcggtttgc
     9001 aggaggtgcg gcggcaccaa tcgccacggg gcgcggaact ggtattcctt aaagcaccag
     9061 acagcgaaga gttaattgaa ctgtgttgct ttcccgcaag cggcccggtg caggtgcagc
     9121 cggatttgac gcacctggcg tttgccgtgg acagcttgga ggaattcggc cgccacctcg
     9181 cgcggttggg tctggcgtat tcggacggcc cacatctcaa cgccgatggc ggcggcctgg
     9241 cctttataga tgccccggaa ggctacgaaa tcgagttgat tcagccgaac cgatcaggcg
     9301 ccgaaggagg gctttgaggc caggggtgaa gctgcgctgc cgccagtttg agttcacttt
     9361 cccgcgcccc gcgctggtga tgggcatcgt gaacgtcacg cctgactcgt tttatgatgg
     9421 cgggcggttt ctggacacgc gggcagcagt ggcccatgcg ctgcagttgg tggaagaggg
     9481 ggcggagatt attgatgtgg gcggcgaatc cacgcgcccg ggcgccgcgc cagtgacgga
     9541 gtccgaagaa ctgcgccggg tgatgccggt aatcgaacaa ttggccgggc ggctcaaagt
     9601 gccgctttcg attgacacgg ccaagcccgg agtcgcccag gcagcgctgg ccgcgggagc
     9661 cagcatcatc aatgacgtcg cggcctatgg cgagcgggag gccatgtggc ggctggccgc
     9721 cgaaacccgg gcgggctacg tgtgcatgca tgcgcagggc accccgcaaa ccatgcagct
     9781 caatcccgcc tataccgatg tcgtccgcga ggtcggcgag ttctttgcgg aacgggtcca
     9841 gcgactgcgc aattgcggcg tattcgggga acagcttata ctggaccccg gaattggctt
     9901 tggcaagacg gccgggcata acttgcagtt gctgggggcg atgcgggctt ttgcaaagtt
     9961 cgagcgcccg ttgctggtgg gcgtttcacg caagtctttt atcgggacgc tgctgggcgc
    10021 ggaaccggcg gaacggctgc cgggggcttt ggcctgcgcc tgcctgacgg tggcggacgg
    10081 ggtgcaattg attcgtgccc atgacgtggc ggagaccgtg caggctattc ggatgaccga
    10141 agccattctg gcgaaacaga actgatacgg ctaccatgtg gcaaaccttg caaacggcct
    10201 ggcgtcccgc gctggagatt ctgattctgg cggcgggaat ctatggcggt ttccggtttg
    10261 tgcgcggcac ccggggcgcg ccggtggtga cgggttttct ggtggcgctg ctggcgctgg
    10321 tgctggtgag ctacctgctc aagttcaagg tgttgcaata cttcctgggc ggattctccg
    10381 cgttcttcgt gctggcggtg ctggtgatct tccagccgga actgcgccgc atgctggcgg
    10441 agcttggcaa cttgcccctc tttgccaccg cgcacgagca acgggaaaac atcgaagtga
    10501 ttattcagac cgtcgagcgg ctggccgacg tgcgcatcgg cgcgctgatt gcggtggagc
    10561 gttccatcca cctgcaagag gcggttgaat ccggcatccg tgtggattgc gaggcaacgc
    10621 cagagatgct ggagacgatt ttcttcccga acaacgcgat ccatgacggc ggggtgatcc
    10681 tgcaaggcga tcgcattgct ttcgcggcct gcatctttcc gctgacgcag cggcaggatt
    10741 tgcacccctc cctgggcacg cggcaccggg cggccatcgg tctcagcgag gaaaccgatg
    10801 cggtggtggt agcggtgtcg gaggagaccg gggcaatttc ctatgcctac aagggccagt
    10861 tgatgcgcgg ggtgacagtc gaagaattgc gcgcctttct gacgtccgtg cttgtcagtc
    10921 ccgttcgctc gcgcaactgg ctgggcgggc cctggtttcg caatgccgac gacgccaaac
    10981 cggggccggt ccatgtggcc cgggcggatt cgccggctcc ctccaccggc aaaagcgcca
    11041 gcctatgatt gccttcctgc gcaacctggt tttcgaggat ttctggctga agctcttctc
    11101 gctggtgctg gcggtgctgg tctggttcat cgtcaccttt gccagccaaa cggatgccca
    11161 aaccgatgag cgcgtgtttt cccacctgcc gattgccgtt gtttccgcga ccgaagatgt
    11221 gcgtgatttc cgggtcagcc ccaatcaagt ccaggtccgg gtgcgcggag actccaaaac
    11281 tctccaaaac gtgcagagca aagacatccg ggcaatcgtg gatctgaccg gggtgacgac
    11341 ggcgcgcgag ctgcgcaagc gcgttgaagt agcggccccg accgggatta cccatctgcg
    11401 cgtggtgccc gaagaggtgc aagtcatttt cccgcccgac cgatagcgcg cgccggccgc
    11461 gttgccggaa cggaataagg ttcggagacg atcccgcggc gagccagagg cccgcgcgta
    11521 ttctccggcg cccactgggg gtaaaacggc ccgaccccgc cccggagaat ccccgatagt
    11581 attttcaggt ttgccccgat tgtcggtttt gattccgcat gcaaaactgt tcgcacgata
    11641 tgcctcgttc ctccaggcgt aagattagta cggtggggcg gttcggacaa ccgaaccgcc
    11701 cttgttttaa tttatggctt ggcgcggaag ccataaaaac aagtgtcagc cccgcccgcc
    11761 gggcctccca ggagcgcgca ttaattcttc gaagcctgca ccacccctgc taaactgaat
    11821 gttttgccat gtctcccctc ccctcacccg actccccgct atctgaactc ccgccctcac
    11881 ttcccgcttc caatcgcaag atcagcctgc tgctggcgga tgaccacccc gttgtgcgcc
    11941 ggggcatcaa aagctgcctg accccctgtc cgagaatcga agtcctgggc gaagccgccg
    12001 atggccagga agccctcagt aaaacgaggg aattgatgcc ggatgtcctg ttgaccgacc
    12061 tggaaatgcc caacctatcc gggctggcgg tggccgaagc gctgcgcaaa gaacattcca
    12121 aaaccagggt gctgattcta tccgcccatg caaaccccac ctatgtgctg cgcagcgccc
    12181 gggcgggcgc cagcggctac gtcctcaaac aggcgccgcc ggacgaactc gtccgggcga
    12241 ttgaaacggt caacgccggc cagccgtttt tcagtccgga ggcgttgcac gccaccctca
    12301 accaatacgt tcgcggttcc ggacaggagt ttcaccccgc cggcttgacc acccgcgagc
    12361 gggaagtcct cgtttacatt gcggaaggac tcagcaataa ggagatggcc gaacgccttc
    12421 acatcagccc gcgcaccgtg gaaacctacc gcgagcgcct gatgcgcaag ctggacatcc
    12481 acaccatcgc cgggctgacc cggttcgcgg ttgcccaagg tttgaccacc attcccgaat
    12541 tggccgttgt ctgacgccca atcagccccg gatcgcccgc ccgcccaggc tgtggcttcc
    12601 cgggaccggc ctcttacggc agccgcaaga tttccgggcg caagctgcct acatgcaggt
    12661 ctggcagccg gcggtccagc gaaccgggca ccatgccttc cccggccaca tggccatcgc
    12721 agaacaccac attcgccttg tgcgcgtgcc ggaagtggcc attggggtaa taggcgcgac
    12781 tggcgtaatt cgtcggcgca tcgagataat agaactcttc caaccgggga ttcgcaggcg
    12841 aggccggagc ctgaaaatcg ttcacctgcg cggcatctcc cagcagggcc tggtccgccg
    12901 ggcgcgtgat ctgcgcaatc cgcaccggcg gtttctttaa cgaaacggaa agcgcgaggt
    12961 tgtaaccgta accataggcc gccccggacg ccttgagctt gaacctggcc aggctatagt
    13021 ccagggctgg gcagatttcc actccgcgcc cgcgcagata gggataaagc gcgcccctcg
    13081 tcgcatcgaa gggccgttgg ccttcctccc cggggcccag ccagccgaac cagcaaagct
    13141 ggcccacgtt cgtttgcccc atcaggtagc ggaaacagtt gccggcgttg tcatcccaat
    13201 aaagctgggc ggccaggcca agctgacgaa gcccgctggc gcacttaacc cggttggccg
    13261 aggcccggcc gtgcgccaaa gccggcaata gcagcgcggc cagaatggcg atgacggcaa
    13321 tgaccgacag caattctatc agcgtaaagg cacgccccgg ctcccggcgc acccgggcac
    13381 ccgcgcggct catggctgct ctgcgcgcac gcggtaaaat gccgcggcgg caggcggttc
    13441 ggcatcctgc aacaccaggt cccctgccac tccctggcgg gtcgcagaga cgggcgtcca
    13501 ggactggaaa tccgtcgtgc gctccagggt gtagcgccag tcggcacggc taaaaaagcg
    13561 ggcctgccaa accccgcccg cgccccagcc gccttgcacc ctgccgattg cccgcgcggt
    13621 gccggcgacg cccaggctgt ccacggtgcc ccgggcccac aggctgcccc aactattggc
    13681 gtcgctgtag ctggcgatgg caatgtgatc cacgcggaag cccagaaatg tcggaggcaa
    13741 atacgcttcc ggcaccgggc caaccgcgat cccccccagc gtgaggttgg tgatcgtggt
    13801 gcggagcgtg cgcgtatccg ccgcatagac caattccaca tggtaaagat tatgggcggc
    13861 caattccatg ccggcaaacc ctcccagcga ccaatggctg ctgtccgtcc ccacccaatc
    13921 caccatcatg gtcgtaaccg aagcccccca ccacaattct ccctccgggt ctggaaagta
    13981 gtcaaactcc accaggttcg gcgaatcagc ccccgtgccc cgcaggaagc ccggttgcgt
    14041 ggcgtcggcc aggttcaaca tccccagggc tagctcgaag ccatagccgc caatcgccgc
    14101 ttcgtgaagt tccaggtcaa aggcaacgct aaaatcatca tccatcgtca gcgtcgcgcc
    14161 cagcggatga caaaagtagc tgttggacaa tgaagagtcc cacgtcactt caaggttctg
    14221 gttggtgggg ttccaataaa acaggttggt atgaccatgc accagccagc cgcgggccgc
    14281 gggatcggcg gcaaaatcct cctgcatact gagcgtctgg gcctgaacgg cccccaccac
    14341 caaaccactg gccagcgcca gcacaaacca acgagctttc cacatgtttc gcatcttact
    14401 gatctttcac aaagccggcg gaacaaaccc gcgcggcggg attaatgttg gagagaccag
    14461 agcgaagccc ggaaaacaaa agcctccaga cccggcaaat cggtgaatga aggcattcat
    14521 caaatcctgg cgcgcactca cgcgccaggc tggccccatc cttctctttg acggagaagt
    14581 tggccccctc aaaccggcca ggcattctgc ctgtccggcc gcgaggccct gacgagcacc
    14641 gccaaaccga tatcctgact ccgggctaga aatctcgctt ccaccttccc agtcttttaa
    14701 ccgaccagtg gctttggaag ctcgcaaccc gttacagtgg cgcaaccgtc cccgattcgc
    14761 acggggttcc ctgacatttg gcagtgtatg cgatgggcaa ccggcccatc agtttcaaag
    14821 aacacctcct aaactatagg atccgccgcc gcttgccaag ccaaatattt tcccttaacg
    14881 taactttcac ggattatgaa gtttgcacaa atctcgaaac gcatcctgtt gctggtggtg
    14941 gtcaacctgc tggtggtgac caccatctcc gtggtgttga gcctgctgcg ggtcgggcgg
    15001 tactttcccg agggcggcct gacccaactc gcgatcattt gcctgctatg gggctttgcc
    15061 ggcgcgttca tttccctggc cctttcccgc ctcatggcca agtggaccat gggcgtgcag
    15121 gtgattcccc cgaacacctc cgaccccgcc ttgcgggaac tggtgcaaac cgtccacggc
    15181 ctggcccgca gcgcgaacct gccccaactt cccgaagtcg gcatctactc atcccccgaa
    15241 gtcaacgcgt ttgccaccgg cccgaccaaa tcccgctccc tggtcgccgt ttcgagcggc
    15301 ctgctccaac gcatgcaccc ccgcgaagtc gagggcgtcc tcggccatga aatcgcccac
    15361 atcgccaatg gcgacatggt caccatgacc ctcatccagg gcgtcatcaa tgcctttgtc
    15421 ctgttcctct cgcgcgtcct ggctttcgtc atcagccagg ccctgcgctc gcgcgacgac
    15481 cgcggcggcg gcggattgct gcaattcttt ctgatcatgg tctttcaatt cgtcttctcc
    15541 ttcctcggca tgctggtggt gtgcgcgttc tcccgctggc gcgagttccg cgccgacgcc
    15601 ggcggcgccc gcctggccgg gcgcgagaac atgattgacg ccctccgcgc cttgcaacgg
    15661 ctccataatc ccgagctggc agccgccgaa gcgcggcacg cccaatcctt ccaggcgctg
    15721 aagatctccg gctcgcgcag ccggttcatg gcgctcctgg cgacccaccc gcccctcgaa
    15781 caacgcatcg cccgcctggc ccagggcgcc gcctgacgcg cggccccgac attccgcgcc
    15841 gggcccgtcc ccgccagccg ccgcccggcg gacgcccttt gcgcttgcat tcgcgctggc
    15901 ttcccgcatc ttgggcgcgc atgatcgcca cgaaacaact ttggacggct ccggcgcccg
    15961 ccgccgtccc cgggctcccg cccgcggagg cggcgcccgg cgcgacgccc gccaacgccc
    16021 cggccttatg atcgaattca acaaccgaat cctgatgacg ggtttcggcg tggtggccca
    16081 ggctgtcctg cccatgctgc tgaagcatgt gcgtgtcccc tgccgaaaca ttacggtcat
    16141 tgactttgcc gactgcgaag ccgagctgcg cccctggatc aaacgcggcg cgcggttcat
    16201 ccgggaacgg gtcaccccca cgaacctgac gcggctgctt tccacgcacg ttggccgggg
    16261 agacctgatc attgacctgg cctggggcat tgagtttttt gaaatcctgg aatgggcgca
    16321 caacaaccag gtgctgtacg tcaacgcctc cctggagtcc tggcattcct cggtggagat
    16381 gcccagcaag tcctccatgg ccaaatcgct ctacgcgaat tacgagcggc tgctgcccat
    16441 tgtcgcccgc tggggcccgg cgaccacggc ggtgattgac cacggctcca atccgggcct
    16501 ggtgtcgcat ttcgccaaac agggcctgct ggacatcggc gagaaaacgc tgcgcgaaaa
    16561 ccacctgccc cgcgcccaga cccgccggct cgaacgcctg atgcaggagc agtcctttgc
    16621 cgagctggcc cgcacgctgg gggtgaaggt catccactgc agcgaatggg acacgcagcg
    16681 cgcctccaaa cccaagcgcc cggacgagtt cgtcggcacc tggagcgtgc aaggcatgtg
    16741 ggatgagtcc atttcgccca gcgaactggg ctggggcacg cacgaaaaat ggcttccccc
    16801 cttcgccacc cgcccggcca ccggccccgc caaccagatc atcctgcctc aaatgggcct
    16861 caacacctgg gttcgctcct gggtgccgca ccaggaaatc gtcggcatga tcgtcacgca
    16921 tggcgaatcg ttcggcctgt cgcacgccct gaccgtgcgg gaagccggcc aggcgatcta
    16981 tcgccccacg gttcactacg cctacatgcc gtgcaacgat tccatcgtct ccctgcacga
    17041 actccgctgc cggaactacg aactgcatcc ccgcacccgc atcctgcgcg acgaaatcac
    17101 cgagggcatg gacctggtgg gcgcgctgat catgggacat cgcttccagt cctggtggac
    17161 cggcagcaag ctgagcatcg ccgcggcgcg gcgcaaggtg cgccattcaa acgccaccgc
    17221 cgtgcaagtg gcggcgggcg ttctggccgg ggtgctgtgg gcgatacgaa acccccagcg
    17281 cggcgtgtgc ttccccgaag acctgccgca tgaggagatt ctgcgcgatg cccgccccta
    17341 cctgggacgg ctcgtctcgg aaccctcgga ctggactccc ctgcggcgcc atcgggtgta
    17401 tttcgatgaa aaccccatgg cgcaaggaga ccagagcgac ccgtggcagt tcaccaattt
    17461 cctcttccgc ccatgacgcc ggccacccgg cgaagcgggg ccatgctcgc cgcgctgctg
    17521 ctgctgggcg cggcgggcgc ggcggcggcg ggcccggtcc gtttcgtgca agaccgcttc
    17581 gtggtgggcc tgtgggtgcc gccggccacc caggagaacc tggatgcgcg ctaccgggag
    17641 atcgccgaag ccaacttcac actggtcgta ggccaatccg gcaccaacac cgcggcccac
    17701 ctcaggcgct gcaaacggtt tgggctgaaa acgctgatcc cggcccacgg ccccgccgaa
    17761 aaactgccgg acggcccggc gtgctggggc tacttgctgc gggacgaacc cggcgcgggc
    17821 gcctttgccg aactggcccg gcgggcggag gaaatccgcg cccggcgccc cggccgcttc
    17881 ggctacgtca acctgttccc caactatgcc ccgcccggcg cgctgggcac tcccacttat
    17941 gaagagcatg tgacccggtt cgtccgggaa gtgaagcccg aggtgctctc gatggatcat
    18001 tacccgctca tgcgccccga aggcgatacc cgcgaccagt attgcgccaa cctcgaatgt
    18061 ttccgccgcc attccctggc cgccggcatc ccgttttgga actacttcta cagcatgccc
    18121 ttcaacgacc gcctcgaccc caccgaagcg caaatccgct ggcagatttt caccgccgcc
    18181 gcctacggcg cccggggcgt gctctatttc tgctattgga cgcccggcct gggagccgcg
    18241 ggcgcgggcg agtttcccaa gggcggcgcc ctcctgacgg cggaagggcg caagacccgc
    18301 cattacgaag aggcccggcg catcaacgcc gaactcaaaa acctcggccc gacgctgatg
    18361 ctactgacca gcaccggcgt gtatcgcgtg accaccgaaa ccaacgcccc ggccctggcc
    18421 ggcagcccgc tccgccagtt ggcgcgggtg ggcggcgacc cgatcggcca cttcatcgtg
    18481 ggcgccttcg cccacgccga tggccggcgc gccgtgctca tcgtcaacca cgattaccgt
    18541 tataccgcct ggccgaccgt gtggtttgac gccgccgcgg aagacgtatg ggaagtcagc
    18601 aaaaccaccg gccgggccgc ccccgtggtg gacgacagcc cggaactgaa agggcttcag
    18661 ctctcgtttg gcgccggtga tgcgcggctg tttctgctgc cgcccgccga acggacgccg
    18721 ctccgttcgc cgcgcggccg cgaatagcct aagtcagaac aatcttgtcg ccctctttca
    18781 gggtcacgat ggccttcttc caattcaacg atcggccggc ctgtttggtg cgctggcggc
    18841 gcagcttgcc gcgcacgttc agcgtgttga cccgcgtcac cttgaccttg aatagctcct
    18901 gcacggccgc gcgaatctgc atcttgttgg cgcggggatc ggccatcacc gtgtattgat
    18961 tatacttctc gccctggcgc gtcccctttt cggttaagcg caccgtccgg atgatttcaa
    19021 aagagttcat gctattcctt gcccaggcgg gcttcaactt gctcgaaggc gttgcgggta
    19081 aacaccagtt tatccgggcg cagcacatcg taggtgctga gcgaatcgcc cgtcgccagc
    19141 gccacgttgg gcacgttgcg cgaggccagc gtcaggttct tgtccggagc ctgcgccaca
    19201 atcagcgcgg tgcccttgag agccagggcg ttgagcaccc cgacaaactc ccgggtcttg
    19261 ccggacccca gcttcaactc gtccaccacc accacgtccc cggcgcgcaa ccgcgctgac
    19321 agcgccttgc gcaacgccag tttgcgggtg cggtgcgaga ccttcttggt aaaatcgcgc
    19381 ggcttggggc cgaacaccac cccgccgccg ctccacaacg gcgagcggaa cgagccggcg
    19441 cgggcgcgcc cggtgccctt ctgccgccac ggcttcttgt tggtccccgc gacttcgccc
    19501 acgttcttgg tgcaggcggt gcccatgcgc tgcgcggcct ggtaggcaac cacggcgtca
    19561 tgcaccgcct gggttccctt cccgtcctcg atgagggcaa acttaacttc cagctcgccc
    19621 tggttatttc ctttgatgtc tttaatggtc agcttcatgg cagcacgtta ctttttcttg
    19681 gcggcgctct tcttgaggtt ttcttcccgt tccgcatggg gtttccagcc cttgggccgc
    19741 ttcttggctt cgcgaatgac cacgtaatcg ccgttggcgc cgggaatggc gccccgaatc
    19801 agcagcacgt tgtcttcctt gtgcacctgg atcacctcga ggttctgcac cgtgcggcgc
    19861 acctgcccca tgtggccggg catcttcgtg ccccgcatca ccgtgcccgg aaacaaccgg
    19921 cagccgatgg cgcccaggcg ccgatgccag cctttggttc cgtgcgtgtt gggccccccg
    19981 ccgaagcgat accgcgccac caccccttca aacccgcgcc ctttggtcag cccgatggcg
    20041 tccacataat cgccgggcgc aaacaaatcc gcgccgacca cgtcccccgg cttcacgtcc
    20101 ttcgtgaaat cgcggaactc ccgaatgcgc ttcaccggcc cgccgttgaa tttcttcaaa
    20161 tgcccgagca gcgccttgct gacccgatgt tctttctggt catcaaagcc cagttgcacg
    20221 gcgttatacc catcgctctg ctgcgtttta cactgcagca cgcgattcgg gccgacctgc
    20281 acgatcgtca ccggggcaat catccccctg gcgtcataga cgcgggtctg gcctaatttc
    20341 tttccaagta atccaagtgg catgatctta aaatccttaa atcttgatgg tgatgtccac
    20401 gcccgccggc aggttgagtt tcttcaactc gtccaccgtc cgggccgtcg gctcgataat
    20461 gtcaatcagc cgcttatgcg tgcgttgctc gaaggtctcc tgtgatttct tgtccgcgtg
    20521 cggagagcgt tgaacggtaa accgctcgat ccgggtggga agcgggatgg gtccgccgac
    20581 gcgggcaccc gtgcgtttga cagtgtccgt gatctcgcgg gcggattggt ctatgacgcg
    20641 atggtcatag gccttcagtc gaatgcgaat gcgttgcgca gtcatacaaa gaacaattgt
    20701 ctattactgg ttttagttaa tttattccgc cccctgtttc agggaacgga gcatggacta
    20761 tagcaaactc cgtccacggt gcaacagatt tttacagaaa tttcggcgca ccgcaaacgc
    20821 ccttgcctgg cggcggacgg gtggccgtga aaaaagaaaa aagcaccggc ccgaaacgcc
    20881 gaacggcggc actggcaaac cgggcggaaa gcaaagcgca aactccgccg ccccgaagga
    20941 aaaagccccc cgcgcgccgc gtgagcaccc gcggccagca aggcgttctc cttcccgttt
    21001 ttccgcccgc cgcgtccctt cacgcggcgt ccagggcaat caagagtcgg attagagtct
    21061 cataaggcac ccgctcccgc cgccagccgc ccaagcctcc ccagccagcc tatggctccc
    21121 aataggccgt gtacaagggg tgtaccaagg atgctcaaag ggggttcatg cttattttcc
    21181 atgcgctcct gccg
//
LOCUS       DAOPQG010000062        21060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3108_length_21060_cov_15.760057, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000062 DAOPQG010000000
VERSION     DAOPQG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 21060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21060
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3108_length_21060_cov_15.760057"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PKI07_08490"
     CDS             <1..762
                     /locus_tag="PKI07_08490"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOC51128.1"
                     /translation="MGLRLRANQYGSTATFPGGVSVSPIGLNLTGDYKLRFDAWLNFN
                     GPAPGGGNGSTQITGAGIGTAGTSAQIAGGVIDSINFGTSGEGGTSADYRAYAPAAQA
                     SYQDNSGVYAAGTTAGVRNNSHAYYAGFGGASAPAAQLALYPQQTGTVAAGALGWEWR
                     DMEITKIGNTVTWHVDNLLLATVDLTTAGTLGGGNILFNQYDINSTVSTDPNSPDLLF
                     GLIDNVRVTMIPEPASGALALLGGLGLFAAWRRRK"
     gene            973..3030
                     /locus_tag="PKI07_08495"
     CDS             973..3030
                     /locus_tag="PKI07_08495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51129.1"
                     /translation="MEFKTNRRSVLVAAALVLGTLWIYWPVQYCEFLNFDDPVYVTQN
                     PIVRAGWSARGICWAFQTSYASNWHPLTWLSHMLDCEFYGLAARGHHLTNVFLHLANT
                     LLLLAFLRRATGAFWPSALVAALFAWHPLHVESVAWVSERKDVLSTCFGLLALLAYGF
                     YVERPGARRYLLALLAFGLSLLAKPMLVTLPGLLLLLDYWPLGRSAPGAGPPAEGRGG
                     GRLRRWRRLVWEKAPFFGLSLVSCVVTFLAQKRGGAVASLIHIPLGERVANAATAYAA
                     YLWQTFWPLELAVFYPHPVHPAGAKVLASLLVLLAVSAGVFLGRRPWLIMGWLWFLGA
                     LAPVIGLVQVGDHARADRYTYLPLVGLFIGIVWTATGFAAARPAWRKPLWGLAACVGV
                     LLGLETRVQLAHWKNSETLFRRALAVTGRNYLAHNNLGNVLDLSGRHEDAKRHYEATL
                     RIRPDFAGTHYNLANVLTREGDFDAALQHYRAALALQPNYADAHHNLGVLLARRGRVD
                     EALEHYQAALRSNPAFADAHNSRGNLLFQQGRLDEAAAAYRAALQTRPDFLDARLNLA
                     LTLQQAGPADEAAQQYAEAVRRHPESPRAHSDWGILLARRGRLPEAEHHLAEVVRLDP
                     QSANARYNLGNVLLEQGKTGAAAAQYAEALRLNPGDAQARQKLERLTSAAAPAEPRLP
                     EPP"
     gene            3203..4516
                     /locus_tag="PKI07_08500"
     CDS             3203..4516
                     /locus_tag="PKI07_08500"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOC51130.1"
                     /translation="MRTLYNILFTIGFVLSAPYYFMRMRRRGNWRQGFGERFGQYSTK
                     LKQAITNRDTIWMHAVSVGEVNVCTQLIRALEPRLPNLKIVVSTTTTTGMGELHSKLP
                     NHISKIYYPIDRRPYVNRALASIRPVAIVLVEAEIWPNFLWRAQKLGIPVFLVNARLS
                     DRSWRGYRRFRFLFKPLFAMLAGVGAQNEADAARLRQLGCRPEAIHMVGSLKFDAARL
                     DERRLLDVPALLRQVGVPAGARLLVAGSTHAGEEVILAELFLRLRQRFPDLFLVLVPR
                     HFERSREVGRDVAARGLKIVYRTEVMNHTRLKPGDADCLIVNSTGELKHFYEQATVIF
                     VGKSLTAQGGQNPIEPGALGKAMVFGPHMQNFAEVVRSFLQQEGAIQARDAAELESAL
                     GELLASPARREQLGRNALKVVRENLGAVIRTVDMIVEQLAPGNGG"
     gene            complement(4543..5412)
                     /locus_tag="PKI07_08505"
     CDS             complement(4543..5412)
                     /locus_tag="PKI07_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOC51131.1"
                     /translation="MKRFSRPNRNQPPGRPGLVFAAILANALSLLVSPAGAAPIQPAS
                     LPAKEKLELYLLLGQSNMAGRGQLADEDQTPHPRVLVFTRQNQWEPAVEPITHDKPAL
                     LGVGPGLAFGKAMARKKPGATIGLVPCALGGTPLRRWQRGGDLYSNAVHRARRAMRDG
                     TLAGILWHQGESDAGAATNASSYGARLGQMVQDLRAELGAPGLPFVAGQIGEFLYDRG
                     PDRSPYAREVNAAIARLPRAVPGTGCARSKGLKDKGDQLHFDAASQRELGRRYAAVLL
                     RLQAKAPPARATP"
     gene            complement(5409..7394)
                     /locus_tag="PKI07_08510"
     CDS             complement(5409..7394)
                     /locus_tag="PKI07_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOC51132.1"
                     /translation="MDFFERQDRARRNTRLLVVYFAGGVVMLIASVYAALLLIFAGLG
                     PRRHFEYHDGDIIRSRAQLSFWNPQLLLGAAAGTLTVIGVGSAFKTWELSRGGSTVAT
                     MLGGRPVSPATTDPDERKLRNIVEEMALAAGLPVPQVYVLPAESGINAFAAGHTHSDA
                     VVAVTAGALRLLTRDELQGVIGHEFSHILNGDMRLNLRLMGLIFGILCLAVIGRILLQ
                     ARSRSSRDRNPLPLLGLALLLIGWVGVFFGRLIQAAVSRQREFLADASAVQFTRNPAG
                     LAGALKKIGGLAHGSQLQAAQASEASHLFFGNGVGESFLHWLDTHPPLAERIRALEPD
                     FDGRFKPVNLEEPAPPATPAAPATPRPPIPFPFPGAPRASAGQAGLAPPVITAPLALA
                     GLGVPTPAHLKYAEEWRGSIPESLRAAAREGLGASALVYALLLSDDEALRRRQLDELD
                     GLTSTIVAQETLRLWPEVQAVAPLAKLPLVDLALPGLRNLSPVQFRQFRAAVQKLVES
                     DGQIDLFEYVLQKIVLRHLEPHFQPARPPVIQYYSLKALAGDCAVLLSALAYLGHDQP
                     DAIAAAFQRGAQPLGYAARAELSLLPEADCELAQVDTALNRLSLAAPQIKKNVLNACA
                     RTVAADGVIQGMEAELLRAIADTLDCPLPPFIPALAA"
     gene            complement(7437..8042)
                     /locus_tag="PKI07_08515"
     CDS             complement(7437..8042)
                     /locus_tag="PKI07_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOC51133.1"
                     /translation="MLKSLLAVPVLVIGGLVLLAILWIIGAYNSLVALRNRFKNAYAQ
                     IDVQLKRRYDLIPNLVETAKGYLKHERGTLEAVIAARNAAATASTGAAQNPGDAAAMK
                     QLSSAETTLAGTLGRLFALAEAYPDLKANTTMITLMEELTSTENKVAFARQAYNDAVM
                     SYNTRRETFPTNLIAGAFNFAPAELFVIEKPAEKEAPRVTF"
     gene            complement(8182..9051)
                     /locus_tag="PKI07_08520"
     CDS             complement(8182..9051)
                     /locus_tag="PKI07_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC51134.1"
                     /translation="MAAFFTWLAAAPAQPANSFPLWPDGAPGALGQGDKDIPTLTPYL
                     PEPDKATGAAIVICPGGGYAGLAAHEGADYARFLNEAGIAGFVLKYRLGSAGYQHPIM
                     LLDAARALRTVRARANQWRLDPKRIGIMGSSAGGHLAATLMTHFNAGKPEAADPIERV
                     SSRPDAVILCYAVITMGEFTHTGSKRNLLGNDPAPELARELSAELHVTRDTPPCFIWH
                     TYEDPAVPVENSLQLALALRRVGVPFDLHIYQKGSHGLGLGTRDWNPERRHPWTRDCV
                     FWLRAQGFARPAD"
     gene            complement(9185..10246)
                     /locus_tag="PKI07_08525"
     CDS             complement(9185..10246)
                     /locus_tag="PKI07_08525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOC51135.1"
                     /translation="MKTFADIGDVLVSVLFLGLPVLIGAAGAWFIRWSGRAGRRRRAA
                     TVILGNALLSAFLVSLLLAVGEFYFRFCYDTTDAFGLTRVNQRWFDRHWRPNNAGFRD
                     NVSDYPPTRTPGRRRITFIGDSFTAGHGIPNVEDRFANQIRHLTQDEVHVMAVNGYDT
                     GDEIFVAEELVRQGYQFDQVVLVYVLNDIGDVTDEAYIVDARVRSNPPTNFFIRHSFF
                     LDTYYYWFRGRFDPDVSNYYQFVRKLYNGPTWEAQQKRLLSLRDFIESHGGRFRVVTF
                     PFLHRLGPGYDYRPVHAQLGQFWRDAGVPHLDLLPLFEAHASANWVVNRHDAHPNQAA
                     HALAAKAIAEFLQQDFPPH"
     gene            complement(10563..13016)
                     /locus_tag="PKI07_08530"
     CDS             complement(10563..13016)
                     /locus_tag="PKI07_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51136.1"
                     /translation="MPCGANSKPAREEPPGCIRLIIVLAGLLLGQAVLYGPSLIGSKL
                     LLPLDILGEPGVYLPPRPDGTYLEPRNLFLSDILYLFEPARRFAVSEFHAGRLPQWGT
                     DNFAGAPFIWPKFSPLLALQYAFASPVALAWSWALAAVVAGLGAYRFFRRLLAVSFWP
                     AAIGAWCYPLTAFFIFWQGYPTGVAVYWLPWILLAIAETARATHRAAPVALSLTTGLV
                     AVSGQLDVAALALLVSGVFALWCLYDAYPRQWLQPAARKAALTLAAGFGLGLLLAAPY
                     ILPFLDYAQTSARMERRFGGEEDHPPVGLAALPQVVLPDLYGSVQSGSFRLANYNEME
                     SSAAAYCGVLATLLVAPVACCSRRHRKINGFFVLIFFLALGWSLNIPGLVHLMRLPGL
                     NMFPHRRLAFAAAFALLSMMVIGLEVLWQGPLRRRGWFGLPPALLAALGLWCGYRIFF
                     LPEPVDTQLAAAILNGKPLPWVRDLAEVRQVQSWFVRHYAAAAGWCGVGLLGWWLLWS
                     GRFWQWHGKPFLAVALVGDLLWFGYGRNAQCDPALYFPPLPALAEVARADPGRVIGCQ
                     CLPANLAALCGLRDIRGYDGMDPKRLTELLMSAADPQSAVIPLAQTQWLIPRVTITPE
                     GTLRLPPVLDFLDVRYVIFRGLPPDNARPAFQSPDYWVMVNSNALGRVFVPRRVEMVT
                     EDSLRLAKLVAADFDPREVAYVERPVHLPGPANGSARILEEAPTRIIVAANMETPGLV
                     VLTDRWDAGWRAYVNGRRVPILRTNHAIRGVLLPAGVSRLEFRYAPASFTWGLGLAGL
                     AAVVLLAWSLFAARRCAPR"
     gene            complement(13089..14930)
                     /locus_tag="PKI07_08535"
     CDS             complement(13089..14930)
                     /locus_tag="PKI07_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOC51137.1"
                     /translation="MKSLIALLLAVASGALPAFAREFHVAPAGNDRDPGSAARPWRTI
                     AAAAQRAQPGDVITIHQGVYRERVAPPRGGASDRQRIVYQAARGETVEIKGSEVVKGW
                     TKAQNDAWTVTLPDSFFGDFNPYRDLIRGDWFNPKGREHHTGAVYLNGHWLAEAARLD
                     EVLKPAGAAPLWFGRVESGRTTLWAQFPGVNPNEQLVEINVRRTVFYPERPGVNYLTV
                     RGLKLRHAATPWAPPTAEQVGLIGTHWSKGWIIEDNEISHSRCSGLTLGKYGDQWDNT
                     SADTAEGYVRTIERALANGWNRETIGHHIVRRNTIHDCEQTGICGSLGAVFSRIESNH
                     IYNIWVQRLFTGAEMAGIKLHAAIDTHIARNRIHHAGLGIWMDWMAQGTRISGNLCYE
                     NSTFDLFVEVNHGPFLVDNNLFLSPISLSDMSEGGAYAHNLFAGQIHSRPEPNRVTPF
                     HPAHTTAVAGLVDVKGGDNRFYNNLLLGKGAGARGEFGLAAYDPREHPLSTGGNVYCH
                     GARPYAREDNALNLPAPDPQMRLVEDGPKVFLQWAWLPAMRNPSARLVTTDRLGRARV
                     SALAYENPDGTPLRIDRDYFGQRRRAAHPTPGPFERLKEGERRLRVW"
     gene            complement(14946..17699)
                     /locus_tag="PKI07_08540"
     CDS             complement(14946..17699)
                     /locus_tag="PKI07_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC51138.1"
                     /translation="MRIIDIQPSGPPGFVSATAARLCRSAFLALLAGGLAGCEMAPPA
                     EVSTRDHVGRVGANRYYNPANQVLTPAGLQIELPGMRPQALALSPNGKLLVTAGKTPE
                     LVVLDPGSGRILQRVPLPSEKELTPAPSTVSDHILQPDKEGQVSFTGLVFSPDGKRIY
                     LANVNGSIKVFGEQDGRVRGLFSIPLPRGAAPRREAEIPAGLAVSRDGKRLYVALNLS
                     NRLAELEADTGKILRLWEVGVAPYDVVLAGGKAYVSNWGGRRPDADSVTGPAGRGTLV
                     RVDPVRFIASEGSVSVIDLAPGADPSGTGKSRLEILTGLHASAMALSPNGRLLVVANA
                     GSDTLSVINTERDELVETFSARQNPGDLFGAQPNALAFDRSGKRLFACNGTQNAVAVF
                     AFAPGNSRLLGLIPVGWFPSAVAPDPQRKKLYVANLKGVAATESLNAAKNTNHNSLQY
                     RGTLSLVDIPSARELAAHTRVALANLRYPLLRQAARPPRPNQPPRPVPERVGEPSVFR
                     HVVYIIKENRTYDQVLGDMPEGNGDPSLCIFGERITPNQHKLAREFVLLDNTYCSGLL
                     SADGHQWADSAMATDYMERSFAGFPRSYPDGMDDNDIDALAYSPGGFIWDNAIAHGKT
                     LRSYGEFAITEAGWADPARKGSPKFLDYYHDFINQTRLTRIRSRPGIESLRPYLATNT
                     VGWSMEIPDIFRAAQFIGELKQFEAHGGFPNLSLICLPNNHTSGTRAGSPTPAAHVAD
                     NDLALGRIIEAISRSRFWPETCVFVIEDDPQAGWDHVSGYRTTAFVASPYTRRGVTVS
                     TQYNQTSLLRTMELMLGLPPMNQLDATATPMSDCFTPTPDFTPFTAVPNNIPLDQMNP
                     DPKQVSDPLLRKHAYASARLPLEQIDQCPEDLLNRILWHAMKGSQAPYPVWAVRLQDD
                     DDD"
     gene            complement(17847..20393)
                     /locus_tag="PKI07_08545"
     CDS             complement(17847..20393)
                     /locus_tag="PKI07_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HOC51139.1"
                     /translation="MALTLYDLLPRDERADNDTLLGRFLEYTEARRLQLYPAQEAAIL
                     ELFEEKNVILNTPTGSGKSLVAAAVHFQAVARGKHSIYTCPIKALVNEKWLGLCREFG
                     PDNVGLSTGDASVNRDAPILCCTAEILANLALREGAAADVQEVIMDEFHYYADRERGV
                     AWQVPLLTLPQARFLLMSATLGDTTFFEAELTRLNGRPTVTVASPDRPVPLEYAYSEL
                     PLAATLESLVAQGKAPVYVVHFTQLEAAQSAQDFTSINVCTREEKNAIGATLEGFKFS
                     SPYGPEIRKWLKHGIGLHHAGLLPKYRVLVEQLAQRGLLKIICGTDTLGVGINVPIRT
                     VLFSRLCKYDGQKTGILSARDFHQIGGRAGRKGFDDRGWVVAQAPEHVVENLKLAEKA
                     ARDGKKVVKRKPPEKNFVNWDQKTFARLRAAQPERLTSRFQVSHGMLLNVLSRPGDGC
                     RALQRLLRDCHETPRAKKEHTRRAWQLFRSLVERRIIEFTPGAGPGKLRVNVELQEDF
                     SMDQTLSLYLLETLPQVDPQQPDYPLVLLTLVESILEDPDIILRKQLDKLKSRKMAEM
                     KMAGLDYEERMAELEKLEHPKPNREFIYSTFNAFADRHPWVGQENIKPKSIAREMFET
                     FRSFSDYIHDYELQRAEGVLLRHLNRVYKVLTQTVPDTAKNNEVREMELYLGTLIRQV
                     DSSLLDEWEKMRDPSFQRAETREARPPGAEEAAADITRDARAFTAAIRHRIFSFLRGL
                     VNADFEQALSHLNPPAASEEPPWTAARLQQALEPYYTEHERICLDPNARNLRHTYVLP
                     AEDARTWRVQQMLVDPDGHNDWVAEFEVDLARSRAASEPVLRLVRLGSLV"
BASE COUNT         3621 a         6702 c         7193 g         3544 t
ORIGIN      
        1 atgggtctgc ggctccgggc caatcaatac gggagtacgg cgacgtttcc cggtggcgtg
       61 agcgtgtcgc caatcggcct taatctcacc ggcgattata agctgcgttt tgatgcgtgg
      121 ctcaatttca acggcccggc tccgggtggc ggcaacggtt ccacccagat taccggggcg
      181 ggcattggca ccgccggcac ctcggctcag atcgccggcg gggtgattga cagcatcaat
      241 ttcggcacga gtggcgaagg cgggacaagt gcggattatc gcgcatatgc gcccgccgcg
      301 caggcgagct atcaggacaa cagtggcgtt tacgccgccg gcaccaccgc gggggtgcgc
      361 aacaattcgc atgcctacta cgccggtttt ggcggggcca gcgcgcctgc cgcgcagttg
      421 gccctttacc cgcagcagac cgggacggtt gcggcgggtg ccctgggctg ggaatggcgg
      481 gatatggaaa tcacgaagat tggcaatacg gtcacctggc acgttgacaa tctgctgctg
      541 gccacggtgg atctgaccac cgccggcacg ttgggcggag gaaacatcct gttcaaccaa
      601 tatgacatca actccacggt ctcgacggat cccaattcgc ccgatctcct gtttgggctg
      661 attgacaatg tgcgcgtgac gatgattccc gagccggctt ccggcgcgct ggcccttttg
      721 ggcggactcg ggctcttcgc cgcatggcgt cggcggaagt aagccctccc aacctttcaa
      781 cctgttcgag ccgcccgcta gccgggcggc ttttgttgtt gcgggcgggc gggctcgcgg
      841 ttagcctggg gcccgcacgg tgcggcatca agggatcatt cgattgggcc cgggcgcgtt
      901 aacgccgggc tggcgttgcc cggccgccag ccgcggcgac ggcggcgaac cgcataaaag
      961 gctttcccac tgatggagtt caaaaccaac cggcgaagcg tcctggtcgc ggccgccctg
     1021 gtcctgggca cgctgtggat ctactggccg gttcagtact gcgaattcct gaacttcgac
     1081 gacccggttt acgttacgca gaacccgatc gtgcgggcgg gatggtcggc ccgggggata
     1141 tgctgggcgt tccagacctc ctatgcgagc aactggcatc ccttgacctg gctctcgcac
     1201 atgctggatt gcgagttcta cgggctggcc gcccgcgggc atcatctgac caacgtcttc
     1261 ctgcacctgg cgaacacgct gctgctgctg gcgtttctgc gccgcgcgac cggcgcgttc
     1321 tggcccagcg ccctggtggc ggcgctgttt gcctggcacc cgttgcatgt ggaatcggtg
     1381 gcctgggtgt cggaaaggaa ggatgtcctg agcacgtgct tcggcctgct cgccttgctg
     1441 gcctatggct tctatgtcga gcgcccgggc gcgcggcgct acctgctcgc cctcctggct
     1501 ttcggcctga gcctgctggc caagccgatg ctggtcaccc tgccgggctt gctgctgctg
     1561 ctcgattact ggccgctggg acggagcgcg cccggcgccg ggccgccggc ggaggggcgc
     1621 ggcggcggcc ggctccggcg atggcggcgg ctggtctggg aaaaggcgcc gttcttcggg
     1681 ttgtccctgg tttcgtgcgt cgtcacgttc ctggcgcaga agcgcggcgg cgcggtggcc
     1741 tccctgatcc atattcccct cggggagcgc gtggccaatg cggcgaccgc ctacgccgcc
     1801 tatttgtggc agacgttctg gccgctggag ctggccgttt tctatccgca tcccgtgcat
     1861 ccggcgggcg ccaaagtgct ggcgtcgctg ctggtgctgc tcgccgtgtc ggcgggagtt
     1921 tttcttggcc gccgcccctg gctgatcatg ggctggttgt ggtttctggg cgccctggcg
     1981 ccggtgattg ggctggtgca ggtgggcgat catgcgcggg ccgatcgcta tacctatttg
     2041 ccgctggttg gcctcttcat cggcatcgtg tggacggcca ccgggtttgc cgccgcccgc
     2101 cccgcctggc gaaaaccgct ttgggggctg gcggcgtgtg tgggcgtgct gctcgggctg
     2161 gaaacccgcg tgcaactggc gcactggaaa aacagcgaga cccttttccg gcgcgcgctg
     2221 gcggtgaccg gccgcaacta cctggcgcac aacaacctgg ggaacgtgct cgatttatcc
     2281 ggccggcacg aagacgccaa acggcattac gaggccaccc tgcgcatccg gccggatttc
     2341 gccggaaccc actataacct ggccaatgtt ctcacccgcg aaggcgactt cgacgccgcg
     2401 ctccagcact atcgcgcggc ccttgccctg cagcccaact atgcggacgc ccaccacaac
     2461 ctcggcgtgt tgctggcccg ccgcgggcgg gtggacgaag ccctcgaaca ttaccaggcc
     2521 gcgctccggt cgaatcccgc ctttgccgac gcccacaaca gccggggcaa cctgctcttc
     2581 cagcaaggcc ggctcgacga agccgccgcc gcgtatcgcg ccgccctgca aacccggccc
     2641 gatttcctgg acgcccggct gaacctcgcc ctcacgctgc aacaggccgg cccggcggac
     2701 gaggccgcgc agcagtacgc cgaggcggtg cggcggcacc cggaatcccc gcgggcgcat
     2761 agcgactggg gaattctcct ggcgcggcgg gggcggcttc cggaggcgga gcatcacctg
     2821 gccgaggtcg tgcgccttga cccccaaagc gccaatgccc gctacaacct ggggaacgtt
     2881 cttctggagc aaggcaagac cggagcggcc gccgcgcagt acgccgaagc cctgcggttg
     2941 aatcccggcg acgcccaggc ccggcagaaa ctggagcggc tgacttctgc ggcggccccg
     3001 gccgaacccc ggcttccgga gccgccgtag ccggcggggg taaatcaggc cgggcgcgcg
     3061 ggcgggcggc ccggggaagc gcggcagggg ccggccgtcc agggagccgc gttttcgccg
     3121 tctttgcggg acggaggaaa ggccttgcgc ggcggcaggg gaacttctag gatgccgagt
     3181 tagtttgctg gtaaagctcg gagtgcgaac tctttacaac atactgttca cgatcggctt
     3241 tgtcttgtcc gcgccgtact actttatgcg aatgcggcgg cgcggcaatt ggcggcaagg
     3301 ttttggcgag cgctttgggc agtacagcac gaaactcaag caggccatca ccaatcggga
     3361 cacgatctgg atgcacgctg tcagcgtggg cgaagtcaat gtctgcacgc agttgattcg
     3421 cgcgctggag ccgcgcctgc cgaatctcaa gatcgtcgtt tcgacgacga ccaccaccgg
     3481 gatgggcgaa ttgcacagca agctgcccaa ccatatcagc aagatctact acccgattga
     3541 ccgccgtccg tatgtcaacc gcgccctggc ctcgattcgc ccggtggcca ttgtgctggt
     3601 ggaggcggag atctggccga actttctctg gcgcgcccag aaactgggca tcccggtgtt
     3661 cctggtcaac gcccgcctgt ccgatcgctc ctggcgcggc taccggcgct tccgctttct
     3721 tttcaagccg ctcttcgcca tgcttgccgg cgtcggcgcg caaaacgagg ccgatgcggc
     3781 ccggctgcgg caactgggct gccggccgga ggcgattcat atggtgggca gcctgaagtt
     3841 tgacgccgcc cggctcgatg agcgccggct gctcgacgtg cccgcgctgc tgcgccaggt
     3901 gggcgtgccg gccggcgcgc gcctcctggt ggccggcagc acgcacgcgg gcgaagaagt
     3961 catcctggcc gagctgttcc tccggctgcg ccagcgtttc cccgacctgt tcctggttct
     4021 ggtgccgcgc cacttcgagc gcagccgcga agtcgggcgc gacgtggcgg cgcgcggcct
     4081 gaagattgtt tatcgcacgg aagtgatgaa ccacacccgc ctgaagcccg gcgacgccga
     4141 ttgcctgatt gtcaacagca ccggcgaatt gaaacacttc tacgaacagg ccaccgtcat
     4201 cttcgttggc aagagcctga ccgcccaggg cggccagaac ccgatcgagc cgggcgcgct
     4261 gggcaaagcc atggtctttg gcccccacat gcagaacttc gccgaagtcg tgcgcagttt
     4321 tctgcagcag gaaggcgcca tccaggcgcg ggacgccgcc gaattggagt cggccctggg
     4381 agaactgctg gccagcccgg cgcggcgcga acagctcggc cggaacgcgc tcaaggtcgt
     4441 ccgcgagaac ctgggcgcgg tgattcggac ggtggacatg attgtggagc agctcgcgcc
     4501 cggcaacgga ggctgaacgg cgaggcggcc cggcgggccc ggtcagggcg tcgcccgggc
     4561 gggcggggct tttgcctgca ggcgcagcag gacggcggca taacggcggc ccagttcgcg
     4621 ctgcgaggcg gcgtcaaagt gcaactggtc gcccttgtcc ttcaagcctt tggagcgcgc
     4681 gcaacccgtc cccgggacgg cgcggggaag gcgggcgatg gcggcattga cctcccgcgc
     4741 atagggcgag cggtccgggc cgcggtcata aaggaactcg ccgatctggc ccgccacaaa
     4801 gggcagcccc ggcgctccca gctcggcccg gagatcctga accatctggc ccaggcgggc
     4861 gccgtacgag ctggcgttgg tagccgcgcc ggcatcgctt tcgccctggt gccagaggat
     4921 gcccgccagc gtgccgtcgc gcatcgcccg ccgggcccgg tggacggcgt tggaatacaa
     4981 atcgccgccc cgctgccagc ggcgcagggg cgtgccgccc agggcgcagg gcaccagccc
     5041 gatggtcgcg ccgggcttct tgcgggccat ggccttcccg aaagccagcc ccggaccgac
     5101 gcccaacagg gcgggcttgt catgcgtgat gggctcgacg gctggctccc attggttctg
     5161 ccgggtgaac accagcacgc gcgggtgagg cgtttggtct tcgtccgcaa gctggccgcg
     5221 cccggccatg ttggactggc ccaggagcag ataaagctcc agcttctctt tggcgggaag
     5281 cgaggccggt tgaatcgggg ccgcgcccgc gggcgaaacc aggaggctaa gcgcgttggc
     5341 gagaatggcc gcgaagacca gccccggccg cccggggggc tggtttcggt tggggcggga
     5401 aaacctcttc atgcggccag ggccgggatg aacggcggca gggggcaatc aagggtatcg
     5461 gcgatggcgc gcagcaattc ggcttccatc ccctggatga cgccgtcggc ggccacggtt
     5521 cgggcgcagg cgttgagcac gttcttcttg atttgcggcg ccgccaggga gagccggttc
     5581 aacgcggtgt ccacctgcgc cagttcgcaa tccgcctcgg gcagcaggct taattccgcc
     5641 cgggcggcat agccgagcgg ctgtgctccc cgctggaagg cggcggcgat tgcgtccggc
     5701 tggtcatggc cgagataagc cagcgccgag agcaggacgg cgcagtcccc cgccagcgcc
     5761 ttcagcgagt aatactggat gaccggcggg cgggcgggct ggaagtgcgg ctccaggtgg
     5821 cgcagcacaa ttttttgcag cacatactcg aacaggtcaa tctggccgtc gctttccacg
     5881 agcttttgca ctgcggcgcg aaactgccgg aactggaccg gcgacaggtt gcgcaggccg
     5941 ggcagggcca ggtccaccag cggcagtttg gcgaggggag cgacggcctg gacttccggc
     6001 cagaggcgga gcgtttcctg ggcgacgatg gtggaggtaa ggccgtccaa ctcgtccagt
     6061 tggcggcggc gcagggcctc gtcgtcgctc agcagcagcg cataaaccaa cgcgctggcg
     6121 cccaggccct cgcgcgcggc ggcccgcagg ctttccggga tggagccgcg ccattcctcg
     6181 gcatacttca ggtgcgccgg cgtgggaacg ccgaggccgg ccagggcgag cggggcggtg
     6241 atgaccggcg gcgccagccc ggcctggccc gcggacgcgc gcggcgcgcc ggggaacgga
     6301 aaaggaatcg gcgggcgcgg cgtcgcgggc gctgccggtg ttgcgggcgg cgccggctcc
     6361 tccaggttta ccggcttgaa ccggccgtca aagtccggtt ccagggcccg gatgcgctcg
     6421 gccagcggcg ggtgcgtgtc cagccaatgc aggaaggatt cgccaacgcc gttgccaaag
     6481 aacaggtggc tggcttcgct ggcctgcgcg gcctggagtt gcgagccatg cgccaggccg
     6541 ccgatctttt tcagcgcgcc ggccaggccc gccgggttgc gcgtaaattg caccgccgaa
     6601 gcgtcggcga gaaactcgcg ctggcggctg acggcggcct ggatgaggcg gccaaagaaa
     6661 acccccaccc agccaatcag cagcagcgcc aggcccagca gtggcagcgg gttccggtcc
     6721 cggctgctgc ggctgcgggc ctggaggagt atccgcccga tgaccgccag gcacaggatg
     6781 ccgaaaatga ggcccatgag gcgcaggttg agccgcatat cgccgttgag gatgtggctg
     6841 aattcgtggc caatgacgcc ctgcagctca tcgcgggtga gcaaccgcag cgcgccggcg
     6901 gtgacggcaa cgacggcatc actgtgggtg tggccggcgg cgaaggcgtt gatgccgctt
     6961 tccgcgggta ggacataaac ctgcggcacc gggaggcccg ccgccagcgc catctcctcg
     7021 acgatgttcc gcagcttgcg ctcgtccgga tcggtggtcg ccgggctgac cggccgccct
     7081 cccagcatcg tggccacagt gctgccgccg cgggacaatt cccaggtctt gaacgcgctg
     7141 cccacgccaa tcaccgtcag cgtgcccgcc gccgccccga gcaggagctg cggattccag
     7201 aacgacagct gcgcccgcga gcggatgatg tctccatcgt ggtattcaaa gtgccggcgc
     7261 ggccccaggc cggcaaagat cagcagcagg gccgcataaa ccgacgcgat cagcatgacc
     7321 acgccgccgg cgaaataaac caccagcagc cgggtgttgc ggcgggcgcg atcctgtcgt
     7381 tcgaagaaat ccatgatcct tggccgggcg ctcccggggc ggcgaaagcc ggggcctcaa
     7441 aacgtcacgc ggggcgcttc cttttcggcg ggcttttcaa tgacaaacag ttccgccggc
     7501 gcgaagttga acgccccggc gatcaggttg gtcgggaagg tctcacgccg ggtgttgtag
     7561 ctcatcaccg cgtcattata cgcctggcgg gcgaaggcaa ccttgttttc cgtggatgtg
     7621 agttcttcca tcagggtgat catggtcgtg ttggccttca gatccggata cgcctcggcc
     7681 agggcgaaga gccgcccgag ggtgccggcc agggtggtct ccgcgctgga aagctgtttc
     7741 atggccgcgg cgtcgccggg attttgcgcc gcgcccgtgc tggcggtggc ggcggcgttg
     7801 cgggcggcga tgacggcttc gagcgtgccg cgctcgtgct tgaggtagcc cttggcggtc
     7861 tcgaccagat tggggatcag gtcgtaacgc cgtttgagct gcacatcaat ctgggcgtag
     7921 gcgttcttga agcggttgcg caaagcaacc agtgaattat aggcgccgat gatccacagg
     7981 atggccagca acaccaggcc gccaatgacc aggaccggaa ccgccaataa gcttttcaac
     8041 atagtaatcc tttcgtttat tgcttaacag ctaggccagg cggcgggcct gggcaattac
     8101 aaagtgcggc gggaaatgga ataaaccggc gcccccgccg cgccggcaac acgccgcggg
     8161 cgggcgggac gcgccttagc cttaatccgc cggccgggca aacccctgcg cccgcagcca
     8221 gaagacgcag tcgcgggtcc agggatgccg ccgctccggg ttccagtcgc gcgtgcccag
     8281 gccgaggcca tgcgagcctt tctggtaaat atgcaaatcg aaaggcaccc ccacgcggcg
     8341 cagcgccagg gccaactgca gactgttctc caccggcacc gcgggatcct catacgtatg
     8401 ccagatgaaa caaggcgggg tgtcccgggt cacgtgcaat tccgcggaca gctcgcgggc
     8461 cagttccggc gccgggtcat tgcccagcag attgcgcttc gagcccgtgt gtgtgaattc
     8521 gcccatcgtg atgaccgcgt agcataaaat gacggcatcc ggccgggagc tgacacgctc
     8581 gatggggtcg gcagcttcag gtttgccggc gttgaaatga gtcatcagcg tggccgccag
     8641 gtggccgccg gccgaagacc ccatgatgcc aatgcgcttc gggtcgagcc gccattgatt
     8701 cgcgcgtgcg cgcaccgtgc gcagcgcgcg ggcggcatcc agcagcatga ttgggtgttg
     8761 gtagcccgcg gagccgagcc ggtacttcag cacaaacccg gcgatgccgg cttcgttcag
     8821 gaaacgcgcg tagtccgcgc cctcatgcgc ggccagtccg gcgtaaccgc cgccggggca
     8881 gatcacgatg gcggcgccgg tcgccttgtc cggctcggga agataggggg taagtgtggg
     8941 gatatcctta tccccctggc ccagcgcgcc gggcgccccg tcgggccaga gcggaaacga
     9001 gttggcgggc tgagccggcg ccgcggcgag ccaggtaaag aatgcggcca taacaataat
     9061 gcgcttcatt gcaccggcag catagccacg cccggaaata ttcgcgatgg aaatatgaag
     9121 tccgtcaggc cgccggtttc ttccggccgg agggctggaa tgcctgtcca atagcatgcc
     9181 cgcttcagtg cggcgggaaa tcttgctgta agaactcagc gatggcttta gccgcgagtg
     9241 cgtgggctgc ttgattcgga tgcgcgtcgt gacgattgac aacccagttt gcggaggcgt
     9301 gagcttcaaa gagcggcagc agatcgaggt gcggcactcc ggcatcgcgc cagaattggc
     9361 ccaactgggc atggactggg cgatagtcgt agccagggcc gaggcggtgg agaaatggaa
     9421 aggtcaccac ccggaaccgg ccgccatggc tctcgataaa gtcccgcaga gatagcagcc
     9481 gcttttgttg ggcttcccag gtggggccgt tatacagctt gcgaacgaat tggtaatagt
     9541 tcgacacatc cgggtcaaac cggccgcgga accagtaata ataagtgtcg aggaagaaac
     9601 tatgccggat gaagaaattg gtgggcgggt tggagcggac ccgggcatca acaatgtagg
     9661 cttcatccgt aacgtcgcca atatcattca ggacatagac caggaccact tggtcgaact
     9721 ggtaaccttg ccgaaccagt tcttcagcga caaagatttc gtcgccggtg tcgtagccgt
     9781 tgacggccat gacatgcacc tcatcctggg tgaggtggcg gatctggttg gcgaaacggt
     9841 cttcgacgtt ggggatgccg tggccggcgg tgaaggaatc gccgataaaa gtgatgcggc
     9901 ggcggccggg ggtgcgagtg ggaggataat cactgacgtt gtcgcgaaac ccggcattgt
     9961 tcgggcgcca atggcggtcg aaccaccgct ggttgacgcg cgtcaggccg aaggcgtcgg
    10021 ttgtatcgta gcagaagcga aagtagaact ccccgacagc gaggagcagg gacaccagaa
    10081 aagctgaaag caaagcgttg cccaggatga ccgtggcggc ccgccgccgc cgcccggcgc
    10141 ggccagacca gcggatgaac caggcgcctg ccgcgccgat gagaaccggc aggcccagga
    10201 acagcacgct caccagaaca tcgccgatgt cggcaaaagt tttcatgcgg atcggggcag
    10261 ggttgtcaga aatccgatgc cggcagttgg tttgggcagg tcgtgcgccc aactccgctt
    10321 ccagcctgga cgattgcttt tcagaacgca gccagcatac tgcattacga ccgtagcaca
    10381 agattccaac acccctcggc caggtggcgc agccgaaaat tcccgcaaag atgcggaagt
    10441 taaccggagt tcgcagggtc gtggggttga gccagcgtca atcagggtgc cggtgctggc
    10501 ggaacccggt ggccggcatc agccgttttt ccatggctgc cctcttccca ccaggtggtg
    10561 gtttagcggg gggcacatcg ccgggcggca aataggctcc aagccagcaa gaccacggct
    10621 gccaaacccg ccaaaccaag cccccaggta aagctggcag gggcgtagcg aaactccagg
    10681 cggctgacgc ccgcaggcag gagaacaccg cggatggcgt ggttggttcg gaggatcggc
    10741 acgcggcggc cattcacata ggcgcgccag ccggcatccc agcgatcggt caacaccacc
    10801 aaccccggcg tctccatatt tgcagcgaca atgatgcggg tgggggcttc ttccagaatg
    10861 cgggcgctgc cgttggccgg gccgggcaag tggacggggc gttcgacgta agcgacttcc
    10921 cgcgggtcaa agtccgccgc caccagtttc gctaatcgca gactgtcctc cgtcaccatt
    10981 tcgacgcggc gcggcacgaa gacacggccc agggcattcg aattaaccat tacccagtag
    11041 tcggggctct ggaaagcggg gcgcgcattg tccgggggca agccgcgaaa gatcacatag
    11101 cggacatcca gaaagtcgag caccggcggc aatcgcagtg tcccctcggg agtgatggtg
    11161 acccgaggga tcaaccactg ggtttgggcc aggggaatca ccgccgattg ggggtctgcc
    11221 gcgctcatta gcagttcggt cagccgtttc gggtccatgc cgtcatagcc gcggatgtcc
    11281 cgcaagccgc acagggcggc caggtttgcc ggcaggcatt ggcagccgat tacccggccg
    11341 ggatcggctc gggctacttc ggccagggcc ggaagcggtg ggaagtaaag ggcgggatcg
    11401 cattgggcgt tgcggccgta accgaaccag agcagatcgc ccaccagcgc caccgccagg
    11461 aaaggttttc cgtgccattg ccagaaccgc ccggaccaca gcaaccacca gcccaacaat
    11521 cccaccccac accagcccgc tgccgccgca tagtgccgca cgaaccatga ctgcacctgg
    11581 cgcacttccg cgaggtcgcg aacccacggc aggggctttc cgttcaggat ggctgccgca
    11641 agttgcgtat ccaccggttc cggcaggaag aatatgcggt aaccacacca aagacccagc
    11701 gcggccagca gggctggcgg cagaccgaac cacccgcgcc ggcgcaacgg accttgccat
    11761 aaaacttcga ggccaataac catcatggac agcagggcaa acgcagcggc aaacgcgagg
    11821 cggcgatgag gaaacatgtt taaacccggc aggcgcatca ggtgaaccag acccgggata
    11881 ttcaggctcc atcccagcgc caggaagaag atgagcacga agaatccatt gatcttccgg
    11941 tggcgccggc tgcagcaggc gactggcgca accagcaggg tggctaaaac accgcagtaa
    12001 gcggcagccg agctttccat ttcattgtaa tttgccaggc ggaagctgcc cgactgcacg
    12061 gagccataca aatccggcag aactacctgg ggcagggccg ccagccccac cggcggatga
    12121 tcttcctcgc cgccgaaccg ccgttccatg cgggcgctgg tttgcgcgta atccaggaaa
    12181 ggcaaaatat acggggcggc cagcagcagc cccaggccga acccggcagc cagggtcagg
    12241 gcggcttttc gggctgccgg ctgcagccat tggcggggat aggcgtcgta caggcaccac
    12301 aaagcgaata ctccagagac caggagcgcc agggccgcaa catcaagctg gccgcttacc
    12361 gcgaccagcc cggtcgttag gctgagggcc accggcgcgg cgcgatgcgt ggcgcgcgcg
    12421 gtttcggcta tcgccagcag aatccagggc agccaataaa cagccacgcc ggtgggatac
    12481 ccttgccaga agatgaaaaa ggcggttagc ggatagcacc aggcaccgat tgccgccggc
    12541 caaaagctga cagccagcag gcggcggaag aatcggtagg cgcccaggcc cgcaacgacg
    12601 gccgccagcg cccacgacca ggccagcgcg accggggagg cgaaggcgta ttgcagcgcc
    12661 agcagcgggg aaaacttcgg ccagatgaaa ggcgcccccg cgaagttgtc agtcccccac
    12721 tggggcagcc gtccggcatg aaactccgac accgcgaagc ggcgcgcggg ctcgaaaagg
    12781 tagaggatat cactgaggaa caggttccgc ggttcgaggt acgtgccgtc cgggcgcggc
    12841 ggcagatata cgccgggttc gccgagtatg tccaggggca gcaatagctt gcttccgatc
    12901 agggacggcc cgtaaagcac cgcctgcccc agcagcaacc ccgccagaac aatgatcagc
    12961 cggatgcagc cgggcggttc ttcgcgggcg ggtttgctgt ttgcgccgca aggcatgggg
    13021 ttattgcggc tggcaccgcc gaagatttcc ggggctgggg atgcgcgcgc gttcaggcat
    13081 acgcctgttt accagactcg cagccggcgt tccccttctt tcaggcgctc gaaggggccg
    13141 ggggttgggt gggcggcgcg gcggcgctgg ccaaagtaat cacggtcaat ccgcaggggc
    13201 gtgccgtcgg gattctcata cgccaaagcg gacacccggg cgcggccgag ccggtcggtg
    13261 gtcacgaggc gggccgaggg gttgcgcatg gccggcaacc aggcccattg cagaaagact
    13321 ttcgggccgt cttccaccag gcgcatttgc gggtccggcg cgggcaggtt gagggcgttg
    13381 tcttcccgcg cataggggcg ggcgccgtgg caatacacgt tgcctcccgt tgacagcggg
    13441 tgctcccggg ggtcataggc cgccagcccg aattctcccc gcgcgccggc gcctttgccc
    13501 agcagcaggt tgttatagaa acggttgtcg ccgcctttga cgtccaccag tccggcgaca
    13561 gccgtggtat gcgccgggtg gaagggggtg acgcggttcg gttccgggcg gctatggatt
    13621 tggccggcaa acagattgtg ggcgtaggcg ccgccttcgg acatgtccga caggctgatc
    13681 ggggacagga agagattgtt atccaccagg aacggcccgt ggttgacctc gacgaacagg
    13741 tcgaaggtgg agttttcgta gcacaggttg cccgagatgc gcgtgccctg ggccatccaa
    13801 tccatccaga tgcccaggcc ggcgtggtgg atccggttgc gcgcgatgtg ggtgtctatg
    13861 gcggcatgga gtttgatgcc cgccatttcg gcgcccgtaa acagccgctg cacccagatg
    13921 ttgtagatgt ggttgctctc gatgcggctg aacaccgctc ccaggctgcc gcaaatgccg
    13981 gtctgctcgc agtcgtgaat ggtattgcgg cggacgatat gatggccgat ggtttcccgg
    14041 ttccagccgt tggcgagggc gcgctcgatg gttcggacat agccctcggc ggtatcggcg
    14101 gacgtgttgt cccattggtc gccgtatttg ccgagcgtga gccccgaaca ccgggaatgg
    14161 ctgatttcgt tgtcctcgat aatccatcct ttgctccaat gcgtgccgat aagcccgacc
    14221 tgctccgcgg tggggggcgc ccagggcgtg gccgcatgcc gcagcttcaa cccccgcacg
    14281 gtgaggtagt tgacgcccgg gcgctccggg tagaacaccg ttcggcgcac gttgatctcc
    14341 accaactgct cgttgggatt cacgcccgga aactgcgccc aaagagtggt gcggcccgat
    14401 tccacccggc cgaaccagag cggagcggcg cccgcgggct tcagcacttc gtccagccgg
    14461 gccgcctccg ccagccagtg gccgttgaga tacacggcgc cggtatggtg ctcgcggcct
    14521 ttgggattaa accagtcccc gcggatgaga tcgcggtaag ggttgaagtc gccgaagaag
    14581 gaatccggca gcgtcaccgt ccaggcgtcg ttctgcgcct ttgtccatcc ctttacaacc
    14641 tcggagcctt tgatttcgac cgtttctccc cgcgcggcct ggtaaacgat gcgctggcgg
    14701 tcggacgcgc ctccgcgggg cggggcgacg cgctcgcgat agacgccttg atggatggtg
    14761 atgacgtcgc ccggctgcgc ccgttgcgcg gcggcggcga tggtccgcca gggccgggcg
    14821 gcggagcccg ggtcccggtc gttgccggcg ggggcgacgt ggaattccct ggcaaaggcc
    14881 ggcagcgcgc cggacgcaac ggccagcagc agagcaatta aggatttcat ggcatcaatg
    14941 ggtaatcagt catcgtcatc atcctggagc cgaaccgccc agaccgggta gggcgcctgg
    15001 gagcctttca tggcgtgcca gaggatgcgg ttgagcaagt cctccgggca ttggtcaatc
    15061 tgctcgaggg gcaggcgcgc cgaggcatag gcgtgcttgc gcaggagcgg gtcggaaacc
    15121 tgtttcgggt cggggttcat ctggtcgagc ggaatgttat tgggcaccgc ggtaaacggg
    15181 gtgaaatcgg gcgtaggggt gaagcagtcc gacatggggg tggcggtggc gtccaactgg
    15241 ttcatggggg gcaggccgag catgagctcc attgtccgca ggaggctggt ctggttgtat
    15301 tgggtgctca cagtcacgcc gcgacgggtg taggggctgg ccacgaacgc cgtggtgcga
    15361 tagccgctga cgtggtccca accggcctgg ggatcgtctt cgatgacgaa gacgcacgtc
    15421 tccggccaga accggctgcg gctgatcgcc tcgatgatcc ggcccagggc caggtcgttg
    15481 tcggccacat gcgcggcggg ggtcggcgag ccggcgcggg tgccgctggt atgattgttg
    15541 ggcaggcaaa tgaggctgag gttggggaac cctccgtgcg cctcgaattg cttgagctcg
    15601 ccaatgaact gcgccgcgcg aaagatgtcc gggatttcca tgctccagcc gaccgtgttg
    15661 gtggccaggt aggggcgaag cgactcgatg cccggccggc tgcggatccg cgtgagccgg
    15721 gtctggttga tgaagtcgtg gtagtagtcc aggaatttgg gggagccttt ccgcgccggg
    15781 tcggcccatc ccgcttccgt aatggcgaat tcgccatagc tgcgcagcgt tttgccgtgg
    15841 gcgatggcgt tgtcccagat gaagcctccc ggcgaatacg ccagcgcgtc aatatcgttg
    15901 tcgtccatcc catccgggta actgcgcggg aacccggcaa acgaacgctc catgtaatcc
    15961 gtggccatgg ccgagtcggc ccattgatgc ccgtcggcgc tgagcaggcc ggagcaatag
    16021 gtgttatcga gcagcacaaa ctcgcgcgcc agtttgtgtt gattgggggt gattcgctcg
    16081 ccaaaaatgc acagcgacgg gtcgccgttg ccctccggca tatcgcccag cacctggtcg
    16141 tacgtgcggt tttccttgat gatatagacc acatgccgga agacgctcgg ctcgcccacc
    16201 cgctcgggca cgggccgggg cggctggttg gggcgcgggg ggcgggcggc ctggcgcagc
    16261 agcggataac gcagattcgc cagcgccacg cgcgtatgcg ccgccagctc gcgcgccgag
    16321 gggatatcca ccagcgacag ggtgccgcgg tattgcaggc tgttgtggtt ggtgtttttg
    16381 gccgcgttca ggctttcggt cgcggccacg cctttcaggt tagccacgta gagttttttg
    16441 cgctgcggat ccggcgccac ggcgctggga aaccagccca ccgggatcag gcccagcagc
    16501 cgcgagttgc cgggagcaaa agcaaagacc gccacggcat tctgggtccc gttgcaggca
    16561 aagagacgtt tgccggagcg gtcaaaggcc agggcgttgg gctgcgcgcc aaacaagtcc
    16621 ccggggtttt gacgggcgct gaaggtttcc accagctcgt cgcgctcggt atttatcacg
    16681 ctgagcgtat cgctcccggc gttggcgacc accagcagcc gcccgttggg ggaaagggcc
    16741 atggcgctgg cgtgcaggcc ggtcaggatt tccagccggg acttcccggt tccggaggga
    16801 tcagcgccgg gggccaggtc aatgaccgaa accgagcctt cgctggcaat gaagcgcacc
    16861 gggtccacgc gcaccagcgt cccgcgcccg gccgggccgg tcacgctgtc ggcgtccggc
    16921 cgccgcccgc cccagttgct gacatacgcc ttgccgccgg ccagcacgac gtcgtagggc
    16981 gcgacgccga cctcccagag ccgcagtatt ttgccggtgt cggcctccag ctccgccagg
    17041 cggttggaga ggttgagcgc cacgtagagc cgcttgccgt cgcgggacac ggccagcccg
    17101 gcgggaatct ccgcctcgcg gcgcggcgcg gcgccgcgag gcagcgggat ggagaacaag
    17161 cccctcaccc ggccatcctg ttcgccaaag accttgatgc tgccgttcac attggccagg
    17221 tagatgcgtt tgccgtccgg agaaaacacc agcccggtga agctgacctg gccttcctta
    17281 tccggctgga ggatgtggtc ggaaaccgtg ctgggcgccg gggtcagttc cttttccgaa
    17341 ggcagcggca cgcgctggag gattcgtccc gagcccggat ccagcaccac cagctcgggc
    17401 gtcttgcccg cggtcaccag cagctttccg ttggggctga gcgccagcgc ctgggggcgc
    17461 atgcccggca gttcgatctg caaccccgcc ggcgtgagca cctggttggc cgggttgtag
    17521 tagcggttcg cccccacgcg cccgacgtgg tcgcgggtgc tgacttccgc cgggggagcc
    17581 atttcgcaac cggccagccc gccggccagc agcgccagaa aggcggaccg gcagagccgg
    17641 gcggcggttg cggagacgaa gccgggcggg ccggaaggtt gaatatcaat gatgcgcatg
    17701 gacatttttc gggcgatgcc ccggttcaaa cgactcccga atcctagccg tttgggccgg
    17761 gagagacaag cccaacgaac cgcgtccgca aggcgggcgc ccggcgtccg cggccggggc
    17821 ggcgttcgcc cgggcggcct tgcggtttag accaggctcc ccagccggac cagccgcagc
    17881 accggctccg aggccgcgcg cgagcgggcc aggtccacct caaactccgc cacccagtcg
    17941 ttatgcccgt ccgggtccac cagcatctgc tggacgcgcc aggtgcgcgc atcctcggcg
    18001 gggaggacgt aggtatggcg gagattgcgg gcgttgggat ccaggcagat gcgctcgtgc
    18061 tcggtataat aaggctccag cgcctgctgc agccgcgcgg cggtccaggg cggctcttcg
    18121 gacgccgcgg gcgggttaag gtgcgacaac gcctgctcga agtcggcgtt gaccaggccg
    18181 cgcaggaagc tgaagatgcg gtggcggatg gcggcggtga aagcccgggc gtcgcgggtg
    18241 atgtcggcgg ccgcttcctc ggcgccgggc gggcgcgctt cccgggtctc ggcgcgctga
    18301 aaggacggat cgcgcatctt ctcccactca tccagcaggc tggaatccac ctggcggatc
    18361 agggtgccga gatagagctc catttcccgg acctcgttgt tcttggcagt gtcgggaacg
    18421 gtttgcgtca gcaccttgta aacgcggttc aggtgccgca gcaggacgcc ctcggcgcgc
    18481 tgcaattcgt agtcatggat gtagtcggaa aaggagcgaa aggtctcgaa catctcgcgg
    18541 gcgatggact tgggtttgat attctcctgc cccacccacg ggtgccgatc cgcaaaggcg
    18601 ttgaaggtcg agtagatgaa ctcgcgattc ggcttgggat gctccagctt ttccagctcc
    18661 gccattcgct cctcgtaatc cagcccggcc atcttcattt ccgccatctt ccggctcttg
    18721 agcttgtcca gttgcttgcg gagaatgatg tccggatctt cgaggatgga ttccaccagc
    18781 gtcagcagca ccagcgggta atcgggctgc tgcggatcca cctgcggcag ggtctccagc
    18841 aagtagagcg acagcgtctg gtccatcgaa aagtcctcct gcagttcgac gttgacacgc
    18901 agcttgcccg gccccgcgcc gggggtgaac tcgataatcc ggcgctccac cagcgagcgg
    18961 aagagctgcc acgcgcggcg ggtgtgctcc tttttggccc ggggcgtttc atggcagtcg
    19021 cggagcagcc gctgcagggc gcggcagccg tcgcccgggc ggctgagcac gttgagcagc
    19081 atcccgtgcg aaacctggaa ccgggaggtc aggcgctcgg gttgcgcggc ccggagccgg
    19141 gcgaacgtct tttggtccca gttgacgaag ttcttttccg gcggcttgcg cttgacgacc
    19201 ttcttgccgt cccgggcggc cttttccgcc agcttgaggt tctcgacgac gtgctccggc
    19261 gcctgggcca ccacccagcc ccggtcatca aaacccttgc gcccggcgcg cccgccgatc
    19321 tggtgaaagt cgcgcgcgct gaggatgccg gtcttttggc cgtcatattt gcagagccgc
    19381 gaaaacagca cggtgcggat gggcacgtta atgcccacgc cgagcgtgtc ggtgccgcaa
    19441 atgattttga gcagcccccg ctgcgccaat tgctccacca gcacccggta cttgggcagc
    19501 agcccggcat ggtgcagccc gatgccgtgc ttgagccact tccgaatctc ggggccgtag
    19561 gggctgctga acttgaaccc ctccagcgtc gcgccgatgg cgttcttctc ctcgcgcgtg
    19621 cagacgttga tgctcgtaaa gtcctgcgcg ctctgggccg cttccaactg ggtgaagtga
    19681 accacataca ccggcgcctt gccttgcgcc accaggctct ccagcgtcgc cgccagcggc
    19741 agctcggaat aggcgtattc cagcggcacc ggccgatccg gggacgccac ggtgacggtg
    19801 gggcgcccgt tcaggcgcgt gagctccgcc tcgaagaacg tcgtgtcgcc cagcgtggcg
    19861 gacatgagga gaaaccgcgc ctgcggcagc gtcaacagcg gcacctgcca cgccacgccg
    19921 cgctcccggt cggcgtaata gtgaaactcg tccatgatga cctcctgcac gtcggccgcg
    19981 gccccttcgc gcagggcgag gttggccaga atctccgccg tgcagcacag gatcggcgcg
    20041 tcgcgattga ccgaggcatc gcccgtgctc aggccgacat tgtccggccc gaactcgcgg
    20101 cacagcccca gccatttctc gttcaccagc gccttgatgg gacaggtata aatggagtgt
    20161 ttgccccgcg cgacggcctg gaaatgcacc gccgccgcca ccagcgattt gcccgacccg
    20221 gtgggcgtgt tgagaatcac gttcttctcc tcgaacaact ccaagatggc cgcctcttgc
    20281 gccgggtaaa gctgcagccg cctggcttcg gtgtattcca gaaagcgccc gagcagcgtg
    20341 tcattatccg cgcgctcgtc ccggggcagc aagtcgtaaa gggttaaagc catgcggatc
    20401 aatcctggcc gcgctcccac ccggcaaggc gggtggggac gggcggcggg gcttccccat
    20461 gccgggctcg tggcaacggc ggcagccgcg gcgtttgcag ggctgccggc gggcgctcgc
    20521 cgggtcgcag ccttcgagag cgcggcgatg ttggtttcat ggggccgcag tataagcgat
    20581 cgtcaagggc ggggcgagga tacttccgcc cgcgagccgg tttgcgaaat tgaccccaac
    20641 cacagaccgc cgctttccgc ctgcgcaagc caaagccgcg ccattcccca ccccgcgcgg
    20701 cccggggtaa agcccgccaa gaccacggga atcccggcta acctgaaccg gaacgacgat
    20761 tcagttgggc agttcaaccg cccacccgca ttccctcgga gccgaggcag ccctgatcgc
    20821 ccaggccata gccccggcaa accccttgtt tagcgaaatc agcggtatcg ctgccgaacc
    20881 ccaaacgcct tttgcttgcg aaatcaataa cttacgcaca tggcaagggg taaaatggag
    20941 gcaaaacctg cccaattccg gccaaagcgc accttgcccc tgccttggaa ataccctgca
    21001 tttaccctga tttcaggccg tgtacaaggg gtgtacaagg gatgcccacc gggagtccat
//
LOCUS       DAOPQG010000063        20992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3125_length_20992_cov_14.058413, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000063 DAOPQG010000000
VERSION     DAOPQG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 20992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20992
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3125_length_20992_cov_14.058413"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1049
                     /locus_tag="PKI07_08550"
     CDS             222..1049
                     /locus_tag="PKI07_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC51140.1"
                     /translation="MNLEDHLGDIIRKARSMSQTSPAVAARAAELSETELATLEESGK
                     VPGNFNLAALAELVGLHPGKLEALARGWRPASKDLSVWRELRCLTTAAGGMAVNCYVV
                     WDEVSREAALFDTGWEAQPIAELVAGNQLQLRHIFITHSHEDHVAALGELRRLYPKVH
                     LHSGARNAPVDQRNRANDFIHLGSLRITHRETPGHAEDGTTYIVGNWPDDAPHVALVG
                     DALFAGSLGRGNQSWDLAHQRVREQILSLPPETLLCPGHGPLTTVAEETAHNPFFLI"
     gene            1093..2031
                     /locus_tag="PKI07_08555"
     CDS             1093..2031
                     /locus_tag="PKI07_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOC51141.1"
                     /translation="MNPATSSREKNSAAFNSVLAAFLLTALKVVVGLLSGSLGILAEA
                     AHSGLDLAAATITLVAVRAAAKPPDRGHDYGHGKVENLAAFLETLLLLATCAWIVRES
                     FLRLTVRQVQVDASVWAFAVMAVSIAVDVSRSRMLYRVAVKHRSQALEADALHFSTDV
                     WSSAVVILGLVGVRLAAWNPRLGFLRQADAVAGLIVAGIVMVVSGRLGLRAIQALLDS
                     SPPGAGEQIKTVVTAMEGVVDCHAVRVRHSGPHYFVDLHITVDGEQSLRAAHELTERV
                     EQAVAELFPEVDVTVHPEPTPAPGNPPPNPLGGTPA"
     gene            2187..3365
                     /locus_tag="PKI07_08560"
     CDS             2187..3365
                     /locus_tag="PKI07_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOC51142.1"
                     /translation="MVALAANVQSSPDRPRLREVGIIIGSLPPGEHNAITDVAGVRVG
                     HTTVVEGDSIHTGVTAILPHGGNVFQERVPAGICVANGFGKLTGVTQIIELGELETPI
                     LLTGTLSVWKVADALVEWLLAQPGMEEVRSINPVVGETNDGFLNDIRKRSIRPDHVTN
                     ALFHATSGAVAEGAVGAGTGTVAFGWKGGIGTSSRKLPAAQGGYTVGVIVQSNFGGDL
                     TINGVRVGRELKSQTSKSENQGTGILIAARTDAPPLGNSGDGSIMIVVATDAPLGSRN
                     LERLASRTMIGLARTGSNMANGSGDYALAFSTAPECRRRRGESVLRTTELANDCLSLL
                     FQAAAEATEEAIGNSLFQATTTRGFRGTAEALPLKRVLALLRQAGALSQDSPPQPKGK
                     "
     gene            complement(3448..5040)
                     /locus_tag="PKI07_08565"
     CDS             complement(3448..5040)
                     /locus_tag="PKI07_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC51143.1"
                     /translation="MADIVLTTLNAKYAHTAFGLRYLLANLGPLQASASLFEFDLCQR
                     PLDIVETLLAQQPQIVGLGVYIWNVAAATEVVATLKRLRPDLTVVLGGPEVSYEVETQ
                     PIVQLADYAIAGEGDLAFAELCGQLLSGQQPDGKIIATAPPDLTRLVLPYDLYNAADI
                     AHRLIYVEASRGCPFGCEFCLSSLDTPVRTVPRARLFDHLLRLMERGARQFKFVDRTF
                     NLNLEDCHAILEFLLAHHRTGHFYHFELIPDRLPESLREVLARFPPGTLQLEIGVQTF
                     NAEAAARIQRRQNYPRLEDNFRFLRNHTKAHLHADLIVGLPGEDLQSFAAGFDRLLAL
                     RPHEIQVGILKRLRGAPIARHDAEWEMVYNPHPPYDILQNRLLDFATLQKLRRFARYW
                     DLVGNSGNFRETLPLIWSRDAEAQRLPNSPALPETDPTSASLSPFTSFLRWSEWLHAR
                     AGRTDGIALTRLAEFLFEYLTADRKLETTSTAAALWRDYQRGGRRDQPSFLKPYLPPT
                     TTRPGSERAPRCSLKRQARHWD"
     gene            complement(5051..6031)
                     /locus_tag="PKI07_08570"
     CDS             complement(5051..6031)
                     /locus_tag="PKI07_08570"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOC51144.1"
                     /translation="MPPIAPSQPHVLVTGGAGFIGSHLVERLLTDGKTVVVVDDLSTG
                     SLDNLKAHPRLRVVVSTVSGCAELPQLADAAESIYHLAAAVGVEMVINAPIHTLLTNL
                     RETEALLAAAAPRRVPVLLASTSEVYGKSQQPAFDEGDDLLIGPPHQTRWGYACSKLM
                     DEFLALAYARERSLPVIVTRLFNTVGPRQTGRYGMVLPRFIAAARANQPLQVYGNGSQ
                     TRCFCLVSDTVEALVRLQNCPAARGEIFNVGGTEEVSMRQLAQVVIEVVGSRSTIEYI
                     PYSQAYGAGFEDMRRRKPQVDKLARLTGFRPATPLRQIIQATSTALSACG"
     gene            complement(6039..6962)
                     /gene="trxB"
                     /locus_tag="PKI07_08575"
     CDS             complement(6039..6962)
                     /gene="trxB"
                     /locus_tag="PKI07_08575"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOC51145.1"
                     /translation="MERVVIIGSGCAGLTAALYTARANLRPLVLTGRQPGGLLTTTTT
                     VENFPGFPEGINGYELMTRMQKQAERFGAQVKFGTLEAANLARPPFQLTVDGELIETE
                     ALIIASGASHRHLGLESERKLENKGVTYCATCDGALPMFRNQPLVVVGGGDSACEEAL
                     YLTRFGSIVYLVHRRDTLRASPIMAERTLAQPKIKPIWDTVVTEVLDVQQDKVTGVRV
                     RNVKTNAESVLECAGLFVAIGHVPNTQLFRGPLDMDENGYLIPRQGAATNVPGIFVAG
                     DCADRVYRQAITAAGMGCAAGIQAERYLAAR"
     gene            complement(7110..7676)
                     /locus_tag="PKI07_08580"
     CDS             complement(7110..7676)
                     /locus_tag="PKI07_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOC51146.1"
                     /translation="MLLAFHKPCGVVSRFTPDGSSHRTLAGFGFPRDVYPIGRLDADS
                     EGLLLLSDEPALNQRLLHPRHAHEREYWVQVERIPTQEALRQLSAGLQVQGRQTMPGR
                     AWLLDPQPEMTPRDPPIRFRKTVPACWIGVELHEGKNRQVRRMTAAIGHPTLRLIRVR
                     LGQLQLRDLPSGAWKPLTAADRKLLLSR"
     gene            complement(7744..13215)
                     /locus_tag="PKI07_08585"
     CDS             complement(7744..13215)
                     /locus_tag="PKI07_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC51147.1"
                     /translation="MKLRFVLQSASLLRVAGPFLCAGLLCVSAAGSPAKTIRLRNETI
                     TTPPASKTFPKAQAGDPAARKPASGLFLIQFEGALEASQRAQLNALGVELLNYVPDDA
                     FIAQFDNAIPSQVQALDFVRYIGPYRPEHKVHPRLAAATRALPKSQRTVAITLMLSPR
                     ATPAEVAQARAFFTSVAHESRLRQGTYLCGTLSPASLEALAQASFVLWLEQAPRRRLV
                     DEAAAKLVGGDDGQIGTPTITQQLGFDGSGVTVSVADTGLDSGNTNALHPDLSGRVVG
                     FQWYGDNILDGADGHGHGTHCAGILAGNAATGETDPEFGGLYGLGVASRAKLFIQRLF
                     DDNGTQADPLPSNETLVQDAVHGGAVIGSHSWGNDVQGEYDADAAQFDELVRDADRWQ
                     PEAQPYIMVFAAGNAGPGSLTMDSPASAKNVIAVGASQNVPSTLPLLYGLYADGPDTM
                     ADFSSRGPCEDGRIKPDVVAPGTWIASAASSAALDMAAVSWMMIDDYYVYMGGTSMAA
                     PHVAGAAAVFVQYYQNLYPNARPSPALVKAALINSADALDSLNGGPGPIPNNEEGWGR
                     VNLASLIITNRLNAPRHYEYVDQTILLTNGGFYTRHILVQGSTQPLKITLAYTDVYGF
                     AGAIPALVNDLDLEVIAPDGTLYRGNQFAGDDSAPNAPSPDILNNVEAVHLTQPAPGD
                     YLVRVRGRHIVQDACPTETAAIDQDFALIISGDLARPGAGTVLLDRPAYTAPGELRLT
                     VFDPARAASNSVSVVLRSTTETNGETVVLQASGNYGAFTGAMATVAGSAAPNGKLELQ
                     HGDSIEAVYEDSAQVERVARAVADLIAPTISNVAVATNMGVLTISWQTPEPARSIIRY
                     RDSAHPAFDLAVTNLSLNTAHSVRLTGLVPGTTYFFYLISTDAAGNATTNNNAGTYFS
                     FIAPSAPTVLLVDDWDTTGGDEDEWGATDIPDSTYTNALNAAGFSYAFWKVAARGSPQ
                     LSDLQPFPVVIWRTTDDAIFYDGPHNTLSPPQQVMVQSYLNGGGSFLLASMEILSRLG
                     NVPFRKNALQVGGFKQNPDPLSSCVDCDEDHGVSAIVGAPGNPVTDGFAANLDYSGYP
                     NFDFEEFMFGPDFSDTFTPTTNAVPILFDAASGRPCGMSYPRVGVDSPGRIAFLSFPL
                     DAVPATGPPPGHRAILLRNLLKFLTPGAHGIGTLSLNNDKYSVPAQVIVEAGDSDRAG
                     QGPLQVTFTTSSRTNQVAVILNETTRPGLFRGFLTLVAAETAPNQLPVSHGDIISATY
                     FDASNNSNVIAQAAIDAQPPLISQVQAVTGFGDATVSWRTSKPADSLVQYGESVLLGR
                     TAHSGTLTTNHAVSVAGLLANRDYYYQVVSRDQAGNTTVDNNEGALYTFTTRPAPRPP
                     WFDSLEDGVGDWTVVPDPLFAEVQWTLGTPANGLMNSAHSGTNAWGSVLHGEPISLAT
                     HSFLISPAIDLSGVSQATLTFWHCLDFDSMFEQGQVRIITSIGSPPAEAPVLEDFSGW
                     STAGWEEVSLPLTAHIGKTVQLVWEYQGVMLGAPHGWLVDDISITGVAGSGTLEISKN
                     LGQGRFTLSGPISRTGTAPLTTITNAPPGPYTVRFSDVSFYQTPHEQSLTLTNGGTLA
                     FTGNYVFLDINDNGIADSWERYYFGLATTNRAPFTDSDGDGMSDYAEFMAGTNPTNAA
                     SKLVFLGTEVLSNQFIRLEWAAIPGRLYQVQTASRLTAAPARPGLSGTVDRLSGSFTL
                     HVHAPTNLPYAIQASSNLSAWTSIYTNANGGGLDWVDPESAASARRFYRTLALSGAVT
                     NANLWTPVTEWLQAAASPMRCIITNANAGLQVFRVEVRP"
     gene            complement(13263..16358)
                     /locus_tag="PKI07_08590"
     CDS             complement(13263..16358)
                     /locus_tag="PKI07_08590"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC51148.1"
                     /translation="MRSKLLFILAGWLLLGIGPAISQSLNLPPRAPGALTGTQFASFI
                     TPMPSPPAAERENWIYAQVMSGNIPDFLRTLAPVTVSAQINGINHTATYYVTPDYLAI
                     GSDADYFLEPGTPLLAQRLCDALGCTLPTRKMVNQIWTQAAVKLAPKTIPPSAEMTTV
                     PVFAEHNYMVYTQRCAYTNSFPLGALVSGNKKDVVISSKIYAKLQPGVPKPVVIYGWH
                     KLDGSPIQPLYNGHEESYADYSHGIRLVQNVMLVDGIPTTVSSVLTNPSLAALLSDEG
                     PAEGTTSTGVIQVPRYSPAPRAPVIMAPPRAQTVLPGASPVFRLLAAGDAPLRYSWRF
                     NGAPLPGATQATLALSNVQAAQAGFYAVVVSNLSGVVTSRPARLRVNTNVHPALFADS
                     LDGNTSAHWDLYWGAGNGVADYTANWAADYGGTAYTFNGFTNIIPPAPNSADDGARAL
                     KFTVNNNDATEAIAAVNLYPRGRLFSNNFALKFDLWLNYPGGAGGAGSTGSTEFAIFG
                     LNHLGTQANWAPASVASSDGVWFAMDGEGGVNTDYRAYVGNLSGGPTDLTVAGASGLT
                     ASNSIAAIYQNLFPAARFESAGAPGKNWVEVELRQTQGILEWRLDGTLIAQRTNTSAF
                     TQGNIMIGYLDPYTSIAKPAKDAFALFANLRVEDLSTPALTPPIIASPPGSQAVGAGG
                     SATFTVTAAGSPPFAYQWRRDGTNLPGATGSSFTLANVQPADGGLYDVLVSNPAGHAA
                     SAPAVLTVAAPEVQFLSASVLTNGQVQLIFSGLPGQSYLIYASTNLVNWTPISVLAVS
                     GGPLPFIDPEAADYPARFYRACAASTQMLADFEAYAPGTQVMFQPPTSSGSTSGFLDS
                     APNKVSITNAFPAGHASDRTLAVAWSFKAGAVNPWLRLTTFNAPHLPNPTIGTNQVLQ
                     FEIYADRALYVGVGFRETSTTAAIGADGGSSGPIEWVGGITDNTVTPPKGHIVPAGQW
                     TTLSFFLPYEPARSFYLGDGVLTSTTGKGVFEHLEFVPGGGLGTYNVWLDNFRVTDLG
                     P"
     gene            16516..16908
                     /locus_tag="PKI07_08595"
     CDS             16516..16908
                     /locus_tag="PKI07_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC51149.1"
                     /translation="MRKRRILVVDDQPMICDAVKMMLDVDGHEVKAAFSGEEALATLQ
                     REEFDLVITDYEMPNMKGDELAAAIKARSPAQPVLMITAYAEMLQALPNALADIDFIL
                     SKPFRLENLREVVAKATSAEPRDQAPAG"
     gene            17002..18069
                     /locus_tag="PKI07_08600"
     CDS             17002..18069
                     /locus_tag="PKI07_08600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC51150.1"
                     /translation="MRQISWRRVGIAGLLLAGLSTQADPVDQYIKSQMEQHRIPGVAL
                     KVIRDGKPIKTAAYGFANLELNVRTRPGTVFEIGSITKQFTAAGILLLAQDGKLSVDD
                     RISRHLKDTPAAWANVTIRHLLTHTSGIKSYTGLDGYQIWRHLTQAQFIQAIGRQPME
                     FAPGESWKYCNTGYNLLGHIIENVSGQDYWDFMSQRIFQPLGMHATTNRLPRLVIPGR
                     AAGYEYTNRVWLNRDADLTEVFAAGAIASTVDDLAKWNAALDGERLLSAASKAQMWTP
                     VKLNDGSTRRYGFGWNVGMVEGHRNIGHGGSTSGFSASLQRFPDDRLAVIVLTNTEQE
                     IATTLARKIATFFYRKDPHAP"
     gene            18059..>18408
                     /locus_tag="PKI07_08605"
     CDS             18059..>18408
                     /locus_tag="PKI07_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOC51151.1"
                     /translation="MPLNPARSHPKNPWIRNGAAARMLIRLTRRRVPVLSRLYLYFMG
                     CDIGLGLPRTVFLPHPMGIVIHRNTRLGDDVVIGHQVTLGGRDLTNAAPHVEDGVYIG
                     AGAKILGGVRIGRGA"
     assembly_gap    18409..18508
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18509..19457
                     /locus_tag="PKI07_08610"
     CDS             <18509..19457
                     /locus_tag="PKI07_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51152.1"
                     /translation="GVETDFLEWWNDRQTADVLHHFGSIPVHTIRLAQEKGWKVVNTI
                     LLSETANRARSALLLRRVLIRAVMAVPQLRSLMGQWHSLRLCDRVLISLEAEKQILVR
                     SYGVSENRVGKLPLGLKESFLRAGPGSRTGDYLISHGTITLIKRSVELARLAREARVP
                     ILFIGKPFAQADDYWKAFRQLVDDQFVRYRPHVGSEPELIHLLQQARGFVLMSRFENW
                     SLAAHEAAACGLPLLLADLPWSRERFGGQVTYFPKGRASAASALRHFYERCPALSAPR
                     VPFHSWREVAEMLRDIYAQMLDSSIPATPGMTAPRQAVR"
     gene            complement(19519..20664)
                     /locus_tag="PKI07_08615"
     CDS             complement(19519..20664)
                     /locus_tag="PKI07_08615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC51153.1"
                     /translation="MKVAFLTTDNRNEYKNYGAPAPYFGAAPTALLQGLAQVPEVEVH
                     VVSCLRQKVASPPQLASNIFYHSLLVPRMGWMRTGYQGCIRAVRRKLKALQPDLAHGQ
                     GTELDCALNAAFSGFPNVLTIHGNMRLIARINRARPFSFLWLAARLEGFTLPRSDGVV
                     CITEHTRQAVMGLARRTWVAPNAVDTSFFEINARPTPGAPAHLLCVAHIYALKNQNAL
                     IRALDPLAGKYPFELHFCGAADERIAYAREFLRLVRARRWCVHHGFAGRQEVQARLLE
                     ATALILPSLEDNCPMAILEAMAAGVPVIAARVGGVPELVEEGKTGFLCNPHEPASMAA
                     AIERVLRDPAWAAAVARQARERARERFHPETIARRHLAIYREVLDQA"
BASE COUNT         3985 a         6119 c         6847 g         3941 t
ORIGIN      
        1 gccgagcttt ctttgtgacc aaatgttacc catttatgcg agtctcaata gatcgtttca
       61 ttgggcaaac ctcccagtca gataccacga tcaatgtccc aacagcattg gctggctcaa
      121 atgcccctcc cgttcaacga gagaaataat ggtgcagccg atgccgcggc cgggaaaggc
      181 cgacgcttga actggctggg catctggata ccatctgact catgaacttg gaagatcatt
      241 tgggcgacat cattcgcaag gcgcgctcta tgtcccagac ttcccctgcc gtcgccgctc
      301 gggctgccga actgtcggag acagaactgg cgacactgga ggaatccggg aaggttcccg
      361 gaaatttcaa cctggccgca ctcgccgaac tagtcggttt gcaccccggc aagcttgaag
      421 ccttggcgcg tggttggcgg cctgcctcca aggatttaag cgtttggcgc gagttgcgtt
      481 gccttactac cgctgcgggt gggatggcag tcaattgtta tgtggtttgg gatgaagtct
      541 cgcgggaggc ggcattgttc gacaccggat gggaggccca gcccattgcc gagttggtcg
      601 ccggcaatca gcttcagctt cgtcacatct tcatcaccca cagtcatgaa gatcatgtcg
      661 ctgccctggg cgagttgcgc cggttgtatc ccaaagtaca cctccattcc ggtgcccgca
      721 atgcccccgt ggaccaacgc aatcgcgcga acgatttcat tcacctcggc agcctgcgta
      781 ttacccaccg cgagacgccc ggccacgctg aagacggcac gacgtacatc gtcggcaact
      841 ggcccgacga tgctccacac gtcgctctgg tgggtgacgc attgtttgcc gggtctcttg
      901 gccgcggcaa ccagtcctgg gatctggccc atcagcgagt gcgtgaacag attctcagcc
      961 taccgccgga aacattgctt tgccccggcc atggcccgct gaccacagtg gctgaggaaa
     1021 cggcccacaa tccctttttt ctgatttgag gcgcgacaga aagatttgcc ccgaatagct
     1081 cttctggccc acatgaatcc ggccaccagt tcgcgcgaaa agaactctgc ggccttcaac
     1141 tccgtgcttg ccgccttttt attgacggcc ttgaaggtgg tggtaggcct gctcagcggc
     1201 agtttgggta tccttgcgga ggccgcacat tccggcctgg atctcgcagc ggcgacgatc
     1261 actttggtgg ctgtgcgagc cgcagccaaa cctcctgacc gtggccacga ctacggccac
     1321 ggcaaggtgg agaacctggc ggcctttttg gaaacgctct tgctgttggc cacctgtgcc
     1381 tggattgtcc gtgaatcgtt tttacggctg accgttcgac aggtccaagt ggatgcatcc
     1441 gtctgggcct ttgcagtcat ggctgtttcc attgcggtgg acgtctcgag gtcccggatg
     1501 ctttatcggg tggcggtcaa acatcgaagc caggcacttg aagccgacgc actgcatttc
     1561 agcactgatg tctggagttc ggccgtggtg attctggggt tggtgggggt gcggctggcg
     1621 gcttggaacc cccggctggg ttttctgagg caagccgacg ccgtggccgg attaatcgta
     1681 gcgggaattg taatggtggt cagcggccgg ctggggctgc gcgccatcca ggctttgctg
     1741 gactcgtctc cccctggtgc gggggagcag atcaagacgg tcgtaacggc gatggaaggc
     1801 gtggtggact gccatgctgt tcgcgtccgt cactcaggac cgcactactt cgtagatttg
     1861 catatcaccg tggacggcga acaatcgctg cgcgcagcac atgagttgac tgaacgagtg
     1921 gagcaagcgg tggcggaact gttccccgag gtggacgtga cagtgcatcc agagccgaca
     1981 ccggctcctg gcaacccgcc gccgaacccc ttgggcggca cgccggcctg atttttgatc
     2041 cgagcgctgt tgtttttttg aggcaggagc tccgtccgca tcgctgcttt cgactgttca
     2101 aaccggcctg ttttgtgaaa agttcctcta tgactaggcg atctgacttt gcacttcctc
     2161 ccgtgtggac tttaattatc gcagctatgg ttgctctggc agcaaatgtc caaagttctc
     2221 cagaccgtcc gcgtttgcgc gaggtgggca taattattgg cagccttcca cccggagaac
     2281 ataatgccat tacggatgtg gctggcgtgc gcgttggcca cactacggtg gtcgagggag
     2341 actctattca tacgggggtg accgcgattc tcccgcacgg tggtaacgta ttccaggagc
     2401 gggtacctgc ggggatttgt gtggccaacg gattcggaaa actgacgggc gttacgcaaa
     2461 tcatcgaact gggcgaatta gaaacaccta tcttattgac aggcaccttg agtgtttgga
     2521 aggttgccga tgctttggtc gaatggctgt tggcgcagcc gggtatggaa gaggtacgtt
     2581 ccatcaaccc ggttgtaggg gagaccaatg atggttttct caacgacatc cggaagcgtt
     2641 ccatccgccc tgaccacgtc actaatgccc ttttccatgc caccagcggt gctgtggcag
     2701 aaggcgcagt gggcgctggc accggcacag tagcttttgg ctggaaaggc ggtatcggaa
     2761 ccagttcgcg gaagctaccc gcggcgcagg gtggctatac cgtaggggtc atcgtgcaaa
     2821 gcaattttgg cggtgacttg accatcaatg gagtgcgtgt tgggcgcgaa ctcaaatcac
     2881 agacgtccaa atcggagaac cagggaacgg gaatattgat agccgcacgg actgatgcgc
     2941 ctccgctcgg caactcgggt gatggcagca ttatgattgt cgtggccacc gatgcgccgc
     3001 tcggctcgcg aaacctggag cgtttggctt cccgaaccat gatcggcctg gcgcgcaccg
     3061 ggtcgaatat ggccaatggt tcgggcgact atgcccttgc gttctcgacg gccccggaat
     3121 gtcgtcgccg gcgtggcgaa tctgtcctgc ggacgactga gttggccaat gactgcctgt
     3181 cgctgctctt tcaggcggcg gcggaagcaa ccgaagaggc catcggcaac tccctttttc
     3241 aagcaactac cacccgggga tttcggggca cggcggaagc tctccccttg aagcgggtgc
     3301 tggcactgct ccgtcaggcg ggcgcacttt cccaagactc tccgccacag cccaaaggca
     3361 agtgaggcgg gcgtagttcg acactgctat tggagcagca attcttctgt tgtctttctt
     3421 cgggagggtg tcgttatgct tgggaagtca atcccaatga cgagcttggc gtttaagtga
     3481 gcagcggggg gctcgttccg agccgggccg agtcgtcgtt gggggcaggt aaggcttcaa
     3541 gaaggaaggt tgatcccggc gcccgccgcg ttggtagtcg cgccacagtg ccgcggcggt
     3601 tgaggtggtt tcgagctttc ggtcggcggt caggtattcg aagagaaact cggccagtcg
     3661 cgttagcgca atgccgtcag tccggcccgc ccgcgcatgc agccattcgc tccagcgcag
     3721 gaaactcgta aaaggagaga gcgatgccga agttgggtcg gtttcaggaa gggctgggga
     3781 attggggagc cgctgggcct ctgcgtcccg actccagatc aggggcaggg tttcgcgaaa
     3841 gttaccgctg ttgcctacca gatcccagta gcgcgcgaat cggcgcagct tctgcagggt
     3901 agcgaaatcc agcaatcggt tttgcaggat gtcgtagggg gggtgtgggt tatagaccat
     3961 ttcccattcc gcatcatgcc gggcgattgg cgcgccccgc agtcgcttca gaatacccac
     4021 ttggatttcg tgaggccgga gggccagcaa ccggtcaaag cctgcagcaa agctctgcag
     4081 atcctctccc ggaagaccga cgatcaaatc cgcatgcaaa tgcgctttcg tgtgattgcg
     4141 caaaaagcgg aagttatctt ccagccgtgg ataattttgg cggcgttgga ttcgggcggc
     4201 ggcctccgca ttaaaggtct gcactccgat ctcgagttgc agcgttccgg gcggaaagcg
     4261 ggcgaggacc tcgcgcaacg attccggcag tcgatccggg atcagttcga agtgatagaa
     4321 gtgtccagtg cgatgatgtg ccagcagaaa ttcgagaatg gcatggcagt cttcgaggtt
     4381 cagattgaag gtgcgatcca cgaacttaaa ctgccgcgcg cctcgttcca tcaggcgcag
     4441 caaatgatca aacaaccgcg cccggggaac ggtgcgcacc ggggtgtcca gggaggacag
     4501 gcaaaactcg cagccaaagg gacagccgcg ggaggcctcc acgtaaatca ggcgatgtgc
     4561 gatgtctgcc gcattataaa gatcatacgg cagcaccaac cgggtgaggt cgggcggcgc
     4621 ggtggctata atcttgccgt cgggttgctg gccggagagc agttgcccgc agagttccgc
     4681 aaaggccaga tctccttcgc cggcgatggc atagtcggct agctgtacga tgggctgcgt
     4741 ctcgacttcg tagctgactt cagggcctcc cagcactacc gtgaggtcgg ggcgcaggcg
     4801 tttgagtgtg gcaacaactt ccgttgccgc ggccacgttc cagatgtaaa cgcccagccc
     4861 aacaatctgc ggttgttgcg ccaggagtgt ttccacgatg tcaagcggac gctggcagag
     4921 atcgaactcg aaaagggagg cgctggcctg cagcggaccg aggttggcga gcaggtaacg
     4981 cagcccgaat gcggtatgag cgtattttgc gttgagcgtg gtcaggacga tatctgccat
     5041 gacagcaaac tcagccgcat gctgagaggg cggtgctggt cgcttgaata atctgtcgca
     5101 gcggcgtggc ggggcggaag ccggtaaggc gtgccagttt gtccacctgc ggtttgcggc
     5161 ggcgcatatc ctcgaaaccg gcgccgtagg cctggctata gggaatatac tcgatggttg
     5221 agcgcgaacc gacaacctcg atcaccacct gagccaactg gcgcattgaa acttcctcag
     5281 tgccgcccac gttgaatatc tcgccgcgcg ccgccggaca gttttgcagg cgcaccagtg
     5341 cttcgaccgt gtcggagacc aggcaaaaac agcgtgtttg agagccgttg ccatagactt
     5401 ggaggggttg gttggcgcgg gcggcggcga tgaaacgcgg cagtaccatg ccgtagcggc
     5461 ccgtctggcg cggccccaca gtattgaaca accgggttac tatcaccggc agcgaacgct
     5521 ctcgtgcgta ggccagcgcc aggaattcat ccattagttt cgagcacgca taaccccagc
     5581 gcgtctggtg cggcggaccg ataagcagat cgtcgccctc atcgaacgcc ggttgctggc
     5641 tcttgccata gacttcggaa gtggaagcta gtaaaaccgg cacgcggcgt ggcgcggccg
     5701 ccgccagcag cgcctccgtc tcgcgcagat tggtaagaag agtatgaatc ggcgcgttaa
     5761 tcaccatctc gactcccacc gccgccgcca agtggtagat ggattccgcg gcatccgcca
     5821 actgtggcag ttccgcgcac ccggaaaccg tggagaccac gacccgcaag cgtggatgcg
     5881 ccttcaggtt gtcgaggctg ccggtggaca ggtcatccac taccaccacg gttttaccat
     5941 ccgtcagcaa ccgctcgacc agatgcgaac cgatgaaccc ggcgccgccc gtgacgagca
     6001 catggggctg gctgggggct atggggggca tggcgctctt aacgcgccgc gaggtagcgt
     6061 tcagcctgga tgccagcggc acagcccatc ccggcggcgg tgatggcttg ccgatagacg
     6121 cggtcggcgc aatcgcccgc gacgaaaatg cccggcacgt tggtagccgc gccttgccgg
     6181 ggaatcaagt agccgttttc atccatatcc agggggcccc ggaaaagttg cgtgttgggc
     6241 acatggccga tagcgacgaa caggcccgcg cactcaagaa cgctttccgc gttagttttg
     6301 acgttccgca cacggacccc agtgactttg tcctgttgca catccaatac ttccgtgacg
     6361 acggtatccc agatgggctt gattttgggc tgggcaagcg tccgctctgc cataatcggg
     6421 gaagcgcgga gggtgtcccg gcggtggacc aggtaaacaa tggagccgaa acgcgtcagg
     6481 tagagggcct cttcgcaggc ggaatcgccc ccccccacca cgaccaacgg ttggttgcgg
     6541 aacatcggca gtgctccgtc gcaggttgcg cagtaggtca cgcccttgtt ctccagcttg
     6601 cgctcgcttt ccaagcccag gtgccgatgg ctggcgccgc tggctatgat gagcgcttcg
     6661 gtttcaatca gttctccatc cacggtgagt tggaacggcg ggcgcgcaag gttggctgct
     6721 tccaacgtgc cgaatttgac ttgcgcccca aatcgttcgg cctgtttttg catgcgggtc
     6781 atgagttcat agccatttat gccctctgga aagccgggga aattctcgac cgtggtggtg
     6841 gtggtcagca aaccgccggg ttgtcgtccg gtcagaacca gcggccgcaa attcgcccgt
     6901 gcggtgtata aagctgccgt caaacccgcg cacccggagc cgataataac aactctttcc
     6961 atacgtttgg gaaggtagtt agagggggag cgattggcaa gggatgagta caaaagcatc
     7021 gcggcaacaa cgcaggcaca cacaggcagc gagcggttgt ttaaggtttg gagcgctacg
     7081 ctggcaagct aatgggagaa caggccgggc tagcgggaca ggagcagctt tcggtcggcg
     7141 gcggtcagcg gtttccaggc gccggaggga aggtcgcgca gttgtagttg accaagacgc
     7201 acgcgaatga ggcgcagcgt ggggtggccg atggccgcgg tcatacggcg gacttgccgg
     7261 ttcttgcctt cgtgcagttc cacaccgatc caacaggcag gcacggtctt gcggaagcgg
     7321 ataggcggat cgcgcggggt catctccggc tgcggatcga gcaaccaggc gcggcccggc
     7381 atggtctgac gtccttgtac ttgcagcccg gcgctgagtt ggcgcagtgc ctcctgggtg
     7441 ggaatccgtt cgacctgaac ccagtattcc cgctcatgag cgtggcgagg atgaagcaat
     7501 ctctgattca gggcgggttc gtcgctgaga aggagcaagc cttcggagtc ggcatcgagg
     7561 cggccgatag gatagacatc ccggggaaag ccaaacccgg ccagggtgcg atgagaagag
     7621 ccgtccgggg tgaaccgaga aacgacgccg cagggtttat gaaaggcgag gagcattgcg
     7681 gtccgaaaga gctgcgggct gagaataaac gcggagcgga tcggctattt tcgctgccac
     7741 gggtcaaggg cggacttcaa cccggaagac ctgcaacccg gcgttggcgt tagtaatgat
     7801 gcagcgcatc gggctggcag cggcttggag ccactcggtg acgggagtcc agaggttggc
     7861 gttggtcacg gcgccagaca acgccaaggt tcgatagaag cggcgcgccg aggcggccga
     7921 ttcgggatcc acccaatcca atccgccgcc gttcgcattg gtgtagatgg atgtccacgc
     7981 cgagagattg gacgaagcct ggatggcgta aggcagattg gtgggagcgt ggacatgcag
     8041 ggtaaaactg ccacttagcc ggtccaccgt tccggacaac ccggggcgag ccggagccgc
     8101 cgtgaggcgg gaggcggttt gcacctggta aagccgtccg ggaatggccg cccactcgag
     8161 gcggatgaat tggttgctca aaacttctgt tcccaggaag accagcttcg atgccgcatt
     8221 ggtggggttg gttccggcca taaactcggc atagtccgac atgccgtctc catccgaatc
     8281 ggtgaatggc gcgcggttgg ttgtggccag gccgaagtag tacctttccc aggagtcggc
     8341 gatgccatta tcattgatat ccaggaagac atagttgcca gtaaaggcga gcgtcccgcc
     8401 attggtgagg gtgagcgatt gctcgtgcgg ggtttgatag aagctgacgt cgctgaaacg
     8461 cacggtataa gggcccggcg gtgcgttggt gatggtagtc aggggagccg tgccggttcg
     8521 gctgataggg ccggacaagg tgaatcggcc ctggccgaga tttttggaaa tctcaagggt
     8581 gccgctgccc gccacgccag tgatgctgat atcatctacc agccagccgt gcggggcgcc
     8641 gagcattacg ccctggtatt cccagaccag ttgcacggtc tttccgatat gagcggtcaa
     8701 gggcagggag acctcttccc agccagcggt gctccagcca ctgaagtctt ccaggacagg
     8761 cgcctccgca ggcgggctgc cgatggaggt gatgattcgc acctggccct gctcgaacat
     8821 ggaatcgaag tcgaggcaat gccagaaagt aagggtggcc tggctgaccc ccgagaggtc
     8881 aatcgccggg ctgatcagaa agctatgggt agccaatgaa atcggctctc catggaggac
     8941 gctgccccag gcgttggtgc ccgagtgtgc gctgttcatc agaccgttgg ccggggtgcc
     9001 gagggtccat tgcacctcag cgaatagcgg gtccggcacc accgtccagt caccaacccc
     9061 gtcttccaga ctgtcgaacc agggcgggcg cggcgccggg cgggtagtga aggtatagag
     9121 cgcgccttcg ttgttatcca ccgttgtgtt gcccgcctga tcgcggctga ctacctggta
     9181 atagtaatca cgattggcca gcaaaccggc cacggagacc gcgtggtttg tggtcaaggt
     9241 gccgctgtgg gcggtgcgcc ccaacaacac agactcgcca tattgaacca atgagtccgc
     9301 cggcttcgag gtcctccagc ttaccgtggc gtcgccaaag cccgtgaccg cctgaacctg
     9361 gctgatgagc ggcggctgag cgtcaatcgc ggcctgggcg atcacattgc tgttatttga
     9421 ggcatcaaaa taggtagcgg agataatatc cccgtggctg accggtaact ggtttggcgc
     9481 ggtttcggcc gcgaccagcg taagaaagcc gcggaacagg ccggggcgcg tcgtttcatt
     9541 caagatgaca gcgacctggt tggtgcggga actggtcgtg aaagtcacct ggagggggcc
     9601 ctggcccgcg cggtcagaat cgcctgcctc aacgatgacc tgggccggaa ccgaatactt
     9661 gtcgttgttg agggagagcg ttccgattcc gtgcgcgccc ggcgtcagga acttgagtag
     9721 attgcgcagc aggatcgccc gatggccggg cggggggccc gtcgccggca ccgcatcgag
     9781 cgggaaagaa agaaaggcga tccgcccggg gctgtccacc ccgacgcgcg ggtagctcat
     9841 tccgcagggc cgtccggaag ccgcgtcaaa gagaatcggc acggcattgg tggttggcgt
     9901 aaaggtgtcg gagaagtccg ggccgaacat gaattcctcg aagtcaaagt tcgggtaccc
     9961 gctataatcg aggttggccg cgaagccgtc cgtaacgggg ttccccggcg cccccacaat
    10021 ggcggacacc ccgtggtctt cgtcgcaatc cacgcaggac gacagcgggt cggggttctg
    10081 cttgaatcca cccacttgaa gcgcattttt gcggaagggc acatttccca atcggctaag
    10141 aatctccatg gaggccagga gaaaggagcc gccgccattc aggtaggact gaaccatcac
    10201 ttgctgcggc ggggaaaggg tattatgcgg gccatcgtag aaaatggcgt cgtcggtagt
    10261 gcgccaaatc acgaccggga agggttgcag atcggagagc tgcggcgaac cccgggcggc
    10321 cactttccaa aaggcgtagc tgaaccctgc ggcgttgagg gcgttggtat aggtgctgtc
    10381 ggggatatcg gtggcgcccc actcgtcttc gtcccctccg gtggtgtccc agtcatccac
    10441 cagcaagacg gtcggagccg aaggcgcgat aaagctgaag taagtccccg cgttattatt
    10501 cgtggtggca ttgccggcgg catcggtcga aatgagatag aagaaatagg tcgtgccggg
    10561 gaccaggcca gtcaaacgga cagaatgggc agtgttcagg gagagattgg tgacggccaa
    10621 gtcgaaagcc gggtgcgcgc tgtcgcgata gcggataatg gaacgggcgg gctcgggtgt
    10681 ttgccaggag atagtgagga ctcccatgtt ggtcgccacc gccacgttgc tgatggtggg
    10741 ggcgatcaag tccgccacag cccgcgccac acgctccacc tgggcgctgt cctcataaac
    10801 ggcctcgata ctatcgccat gctggagttc caatttgccg ttgggggcgg cgcttcccgc
    10861 caccgtagcc attgcgcctg tgaatgcgcc gtagttgccg gaggcctgga gtacgaccgt
    10921 ttccccgttc gtctcggtcg tgctgcgcag cactacgctg acggaattgc tggcagcgcg
    10981 ggccgggtcg aaaacggtca gtcgaagctc gcccggcgcg gtataggccg ggcgatccaa
    11041 cagcacggtt cccgctcccg ggcgcgccag gtccccggaa atgatcagcg cgaagtcctg
    11101 gtcaatggca gccgtttccg tcgggcacgc atcctgcaca atatgccggc cgcggacgcg
    11161 caccaggtag tcgcccggcg ccggctgcgt cagatgcacg gcttccacgt tgttgaggat
    11221 gtcgggcgag ggggcattgg gcgccgaatc atcacccgca aactggttgc cgcggtagag
    11281 cgtcccatcg ggggcgataa cttccaggtc caaatcattc accaatgcgg gaattgcccc
    11341 ggcaaaaccg taaacgtcag tgtaggccag ggtgattttt agcggctgcg tcgagccctg
    11401 gaccagaatg tggcgcgtat agaatccgcc gttggtcagt agaatcgttt ggtccacata
    11461 ttcgtagtgg cgcggggcat tcaggcggtt ggtgatgatg aggctggcca ggttgacgcg
    11521 gccccagcct tcttcattat tggggattgg gccggggccg ccgttcaacg agtcgagcgc
    11581 gtccgccgaa ttgatcagcg ccgccttgac cagcgccggc gagggccgcg cgttgggata
    11641 caggttttgg tagtattgga cgaacaccgc cgccgcgccg gcgacatgag gcgccgccat
    11701 gctggtgccg cccatgtaca cgtagtagtc atcaatcatc atccaggaga ccgcggccat
    11761 atccaacgcc gccgaggacg cggcggaggc gatccacgtg ccgggcgcga cgacgtccgg
    11821 cttaatgcgg ccgtcttcac aggggccgcg actcgagaag tccgccatgg tatccggtcc
    11881 gtcagcataa agcccgtaga gcaacggcag ggtggagggc acgttctggc tggcgcccac
    11941 ggcgatcacg tttttcgctg aggccgggct atccattgtg agcgacccgg ggccggcgtt
    12001 gccggccgca aacaccatga tatacggctg cgcctcgggc tgccagcggt cggcgtcccg
    12061 caccagttcg tcgaattgcg ccgcatcggc atcgtattcg ccctgcacat cattgcccca
    12121 gctatgcgag ccgatgacag ccccgccatg cacggcatcc tgcaccaggg tttcgttgct
    12181 gggcagcggg tctgcctggg ttccgttgtc gtcgaagagc cgctggatga acagcttggc
    12241 ccgggaggcg acgcccaggc catacagccc gccgaattcg gggtcggtct cgccggtggc
    12301 cgcattgccg gcgagaatgc ccgcgcagtg agtgccgtgc ccgtgtccat ccgccccgtc
    12361 caggatattg tcgccatacc attgaaaacc gactacccgc ccgctcaagt ccggatgcaa
    12421 agcgttcgta tttccgctgt ccagtcccgt gtccgccacg gaaaccgtga cgccggagcc
    12481 gtcgaacccg agctgctggg tgattgtggg cgtgccgatc tggccatcat cgcctccgac
    12541 cagcttggcc gccgcctcgt ccaccagccg gcggcgcggg gcttgctcca gccacagcac
    12601 gaaactggct tgcgccaacg cctccaggct ggccggggag agggtgccgc ataggtacgt
    12661 accctgccgc aagcggcttt catgcgccac ggaggtgaag aacgcgcggg cttgggccac
    12721 ctcggctggc gtggcacggg gcgagagcat aagagtgatg gcaacggtgc gctgcgactt
    12781 cggaagcgcg cgggtggcgg ccgccagtcg cggatgaact ttatgctccg gacggtaagg
    12841 accgatatag cgcacgaaat ccagcgcctg aacctggctc ggaatggcgt tgtcaaattg
    12901 cgcaataaat gcatcgtcgg gcacgtaatt gagcaactcg accccgagcg cgtttaactg
    12961 agcgcgctgg cttgcctcca acgcgccctc gaattggatc agaaaaaggc cggaggcggg
    13021 tttccgggcg gcggggtctc cggcctgcgc tttagggaaa gtcttcgacg cgggaggggt
    13081 ggtaatcgtc tcgttgcgca gccgaatggt ttttgccggg ctgccggccg ccgaaacgca
    13141 gagcagtccc gcgcaaagga aggggccggc gacgcgcagc agcgacgcgc tttgcagaac
    13201 gaaacgaagt ttcacgcggt tattgtaatc gcgtctcgcc gctaaaacaa gcgccggcgc
    13261 ggctaaggcc ccaggtcggt cacgcggaaa ttatcgagcc agacattata ggtgcccagg
    13321 cctccgccgg ggacaaactc gaggtgctcg aacacccctt tgccggtggt ggaggtcaac
    13381 accccgtcgc ccaggtagaa actgcgcgcc ggttcataag gcaggaagaa actcaacgtc
    13441 gtccactgtc ccgccgggac gatatgccct ttgggcggag tcaccgtgtt gtcagttatc
    13501 cccccaaccc attcaatcgg gccgctgctc ccgccgtcag cgccgatggc ggcagtcgtg
    13561 ctcgtctcgc ggaagccgac gccgacatac agcgcgcggt cggcatagat ctcgaattgg
    13621 agcacctggt tggtgccaat ggtggggttg ggcaggtggg gggcgttaaa ggtcgtcaga
    13681 cgcagccagg ggttgacggc gccagccttg aaactccagg cgacggccag ggttcggtcg
    13741 ctggcatggc ctgcgggaaa ggcattggta atgctgactt tattgggggc gctgtcgaga
    13801 aagccggacg tcgaaccgga ggaggtgggc ggctgaaaca ttacctgggt tcccggcgcg
    13861 taagcctcga aatccgccag catctgagtg cttgccgcgc acgcgcgata gaagcgcgcc
    13921 ggataatcag ccgcctcggg gtcaataaag ggcagggggc ccccgctgac cgcgagcacg
    13981 gaaatcggcg tccagttcac caggttggtg gaggcataaa tgaggtagct ctgtccgggc
    14041 aggcctgaga aaatcagttg cacctgcccg ttggtcaata cggaggcgga gaggaattgc
    14101 acttcgggcg ccgccaccgt cagcaccgcc ggggcgctcg ccgcatggcc ggcggggttg
    14161 ctgaccagga catcataaag gccgccatcc gcgggttgca cattcgccag cgtgaacgag
    14221 gagccggtgg cgcccggcag gttcgttccg tcgcggcgcc attggtaggc gaagggaggc
    14281 gacccggcgg cggtgacggt gaaagtggcg ctgccgcccg cgccgaccgc ctggctgccc
    14341 ggcggggagg caataatcgg aggcgtgagc gccggggtgc tgaggtcttc cacccgcaaa
    14401 ttggcgaaca gcgcaaaggc atcctttgcc ggtttggcga tggaggtgta aggatccagg
    14461 tagccgatca tgatattgcc ctgggtaaag gccgaagtgt tggtgcgctg ggcgatgagg
    14521 gtgccgtcga gccgccactc caggatgccc tgggtttggc gcagttccac ttccacccag
    14581 tttttgcccg gcgcgcccgc gctctcgaag cgcgccgcgg gaaagaggtt ctgatagatg
    14641 gcggcgatgc tgttggacgc ggttaaaccg ctggcgccgg ccacggtcag atccgttggc
    14701 cccccggaga gattccccac ataagcccgg taatcggtgt tcacgccgcc ctcgccgtcc
    14761 atggcaaacc ataccccgtc cgatgacgcg acgctggccg gagcccagtt tgcctgggtg
    14821 cccaggtgat tgaggccgaa gatggcgaac tccgtgctgc ctgtcgaacc ggctccgccc
    14881 gcgcccccgg gatagttaag ccacaagtca aacttcaggg cgaagttatt gctgaaaagc
    14941 cgcccgcggg ggtagaggtt caccgcggca atcgcctcgg tggcgtcgtt gttgttgacg
    15001 gtgaacttga gggcgcgcgc gccgtcgtcg gcggaattgg gcgcgggcgg aatgatattg
    15061 gtgaagccat taaaggtgta agcggtgccg ccgtagtcgg cggcccagtt ggccgtgtag
    15121 tcggctacgc cattgccggc gccccaatat aaatcccagt gggcggacgt attgccgtcc
    15181 agggagtccg caaaaagagc cgggtggaca ttggtgttga cgcgcaaacg cgccggccgg
    15241 ctggtgacga cgccggagag gttggaaacc acgacggcgt agaaccccgc ctgggcggcc
    15301 tggacattgc tgagggcgag ggtggcctgg gtcgcgccgg gcagcggcgc cccattgaac
    15361 cgccagctat agcggagcgg tgcgtcgcca gccgccagca accggaaaac cgggctggcg
    15421 ccggggagca cggtctgggc gcggggaggg gccatgatca ccggggcccg aggcgcgggg
    15481 gaataacgcg gcacctggat cactccggta ctggtcgtcc cttccgcggg cccctcatcg
    15541 ctcaacagcg ccgccaggct gggattggtc aggacgctgc tgacagtggt ggggatgccg
    15601 tccacgagca tcacgttttg gaccaggcgg atgccgtggc tgtagtcggc gtacgattcc
    15661 tcatggccgt tgtataaggg ttggattggg gagccgtcga gcttatgcca gccgtaaatc
    15721 accaccggtt tgggcacccc gggttgaagc ttcgcgtaga ttttcgagga gataaccacg
    15781 tcctttttgt tgccgctgac taatgctccc agcgggaacg aattggtata ggcgcagcgc
    15841 tgggtataaa ccatgtaatt atgctccgca aacacgggca cggtggtcat ctcagcgctg
    15901 ggaggaatgg tcttcggggc cagtttgacc gcagcctggg tccaaatctg attcaccatc
    15961 ttgcgcgtcg gcagcgtgca gccgagcgcg tcgcataaac gctgcgccag caaaggggtg
    16021 cccggttcga gaaaatagtc ggcgtcactc ccaatcgcca ggtagtccgg cgtgacatag
    16081 taggtcgccg tgtggttgat gccgttgatt tgcgcgctca ccgtgacggg cgccagggtc
    16141 cgcaaaaaat cggggatatt gccgctcatg acctgcgcgt aaatccagtt ttcgcgctcc
    16201 gccgccggcg gggagggcat gggcgtgatg aagctggcaa actgagtgcc ggtcagcgcg
    16261 ccgggagcac ggggcggcag gttgaggctt tgagaaatgg ccgggccaat ccccagcagc
    16321 agccatcccg ccaaaataaa caggagtttc gatctcatgg gccactcacg cgcgcgttca
    16381 gttaatttcc aagcccaaag atagccgatt cccgagcgga gcgcgagcaa aaacaaggag
    16441 aaaaaacact ggccatgccc gcccaatgcc gctacgattt gacctttagt gatggcggta
    16501 ttaaagggat aagctatgcg gaagcggcgt attttggtcg tagatgacca gcctatgatt
    16561 tgtgacgcgg tgaagatgat gctggacgtg gacggccatg aagtcaaagc cgcctttagc
    16621 ggcgaggaag cgctggccac gctccagcgc gaggaattcg acctggtcat cacggattac
    16681 gagatgccga acatgaaggg ggacgagttg gccgcggcca tcaaggcgcg atctccggcg
    16741 cagccggtgc tcatgatcac ggcttacgcg gaaatgctgc aagcgttgcc caatgcgctc
    16801 gcggacatag attttatcct cagcaagccg ttccgcctgg aaaacctgcg cgaagtcgtt
    16861 gccaaagcga catccgccga accccgggac caggccccgg ccggctaaac cacggggacg
    16921 cggcgcccgg ttggggggcc gcgcggggcc gggctgagtt tgaggtggct tgcgccgcca
    16981 gtttcgggct actctgcacc catgagacaa atcagttggc gccgcgtcgg gattgccggc
    17041 ctcctgctgg cgggcctgtc cacgcaggcg gacccggtgg atcagtatat taagagccag
    17101 atggaacagc accggattcc cggggtggcg ttgaaggtca tccgggacgg caagccgatc
    17161 aagacggcgg cctacggttt cgccaacctg gaactgaatg tgcggacccg gcccgggacg
    17221 gtgttcgaga ttggctcgat caccaagcag ttcaccgcgg cggggatttt gctgctggcc
    17281 caggacggca aactgtccgt ggacgaccgg attagccggc acctgaagga cacccccgcg
    17341 gcatgggcca acgtgaccat tcggcacttg ctgacgcata cctcggggat caagagctac
    17401 accgggctgg acggctacca gatctggcgg cacctcaccc aggcgcagtt cattcaggcg
    17461 attggccggc agcccatgga gttcgcgccg ggggaatcct ggaaatactg caatacgggc
    17521 tataatcttc tgggtcacat catcgagaac gtgagcggcc aggattactg ggattttatg
    17581 agccagcgca tctttcagcc gctggggatg cacgcaacga ccaatcgcct gccgcggctg
    17641 gtgattcccg ggcgcgccgc cggctacgaa tacacgaacc gggtgtggct caatcgcgat
    17701 gccgacctga cggaagtctt tgccgccggc gccatcgcgt ccaccgtgga cgacctggcc
    17761 aagtggaatg cggccctgga cggcgagcgc ctcctgagcg ccgccagcaa ggcgcagatg
    17821 tggacgcccg tgaagctgaa tgatggctca acccggcgct acggcttcgg gtggaacgtg
    17881 gggatggtcg agggacaccg gaacatcggg cacggcggat ccacgtccgg cttttccgcc
    17941 tcgcttcaac gcttcccgga cgatcgcctg gccgtcatcg tcctcaccaa caccgagcag
    18001 gaaatcgcga cgaccctggc ccgaaaaatc gcaaccttct tctaccggaa agaccctcat
    18061 gccccttaac cccgcccgat cgcacccgaa gaacccctgg atccgcaacg gcgcggcggc
    18121 gcggatgctc atccgcctga cccggcgccg cgtgccggta ttgagccggc tctatttgta
    18181 tttcatgggc tgcgacatcg gcctggggct tccccggacg gtgtttctgc cgcaccccat
    18241 gggcattgtc atccaccgca acacgcgcct cggcgatgat gtggtcattg gtcatcaggt
    18301 caccctcggc gggcgggact tgaccaacgc cgcgccccac gtcgaggatg gggtctatat
    18361 tggggcgggg gccaaaatcc tgggtggagt tcgcattggt cgtggcgcnn nnnnnnnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gcgtggagac ggattttctg gaatggtgga
    18541 atgatcggca aaccgcggat gtccttcacc atttcgggtc tattccggtg cacacaatcc
    18601 ggctggccca ggagaaaggc tggaaagtgg taaacaccat cctgctttcc gaaacggcca
    18661 accgggccag aagcgctttg ctgctgcgcc gggttttaat ccgcgcagtg atggcggtcc
    18721 cgcagttgcg gtccttgatg gggcagtggc attcattgcg gctctgcgac cgcgtgttga
    18781 tttccctgga ggccgagaag cagattcttg tccgctctta cggagtatcg gaaaaccgcg
    18841 tgggcaagtt gccgctgggg ctcaaggagt cctttctgag ggccggcccc ggctcgcgga
    18901 ccggggatta tctgatctcc catggcacca ttacgctaat caagcgctcg gtggaattgg
    18961 cgcggttggc gcgcgaagcc cgggtgccaa tcctctttat tggcaagccc ttcgcccagg
    19021 cggatgacta ctggaaagca ttccgccaat tagtggacga ccagttcgtg cggtatcgtc
    19081 cgcatgtcgg gagcgagccg gagttgatcc acctgctgca gcaggcgcgg ggatttgtcc
    19141 tgatgagccg tttcgagaac tggagcctgg cggcgcacga ggcggcggct tgcgggttgc
    19201 cgctgttgct ggcggacctt ccctggtcgc gcgagcgttt tggcggccag gtcacttact
    19261 ttcccaaagg gcgcgcgtcg gccgcttccg cgctgcggca cttttacgag cggtgtccgg
    19321 ccttgtcagc gccccgggtg cctttccata gctggcgaga ggtcgcggag atgctgcgcg
    19381 acatctatgc gcagatgctc gattcatcta tccccgccac ccccgggatg accgccccgc
    19441 ggcaggcggt gcgctgaagg ccgttcgcgc gaaacgatgg gcagcggccc ttggggctga
    19501 gggcaagaag gagcggaact acgcctggtc cagcacctcc cggtagatcg ccaagtgccg
    19561 ccgggcaatg gtttcgggat gaaaacgctc ccgcgcccgc tcccgggcct ggcgggccac
    19621 ggcagccgcc cacgccggat cgcgcagcac tctttcaatc gccgccgcca tgctcgcggg
    19681 ttcgtgcggg ttgcataaga acccggtttt gccttcctca accagttccg gcacgccgcc
    19741 gacccgggcg gcgatcaccg gcactcccgc cgccatggct tcaaggatgg ccatggggca
    19801 attgtcttcg agcgagggca gaatcagcgc cgttgcctcg agcaggcggg cctgaacttc
    19861 ctgccgcccg gcaaatccgt ggtgcacgca ccagcgccgc gcccgcacca ggcgcaggaa
    19921 ttcccgggcg taagcgatgc gctcgtcggc ggcgccgcag aaatgcagtt cgaacgggta
    19981 ttttccggcc aaaggatcaa gcgcgcggat caaggcgttc tggttcttca acgcatagat
    20041 atgggccacg cacaaaaggt gcgcgggggc gcccggagtg gggcgtgcgt tgatctcgaa
    20101 gaacgacgta tccaccgcgt tgggcgccac ccaggtgcgc cgggccagcc ccatgaccgc
    20161 ctggcgggtg tgttccgtga tgcataccac tccatccgaa cggggaagcg tgaatccttc
    20221 cagccgcgcg gccagccaca gaaaggaaaa gggccgggcc cggttgatcc gcgcgatcag
    20281 ccgcatattg ccatgaatgg tgaggacatt gggaaacccg gaaaaggcgg cgttaagggc
    20341 gcaatccagt tccgttcctt gcccatgcgc caaatcaggc tgaagcgctt tcagctttct
    20401 ccggacggcg cggatgcagc cctggtagcc ggtgcgcatc cagcccatcc gggggaccag
    20461 gaggctgtga tagaagatat tggaggccag ttggggcggc gacgcaacct tctgacgcag
    20521 acaggaaacc acatgcactt ccacttccgg cacctgcgcc aacccctgga gcagcgccgt
    20581 gggcgccgcg ccgaaatacg gggcgggtgc tccgtagttc ttgtattcgt tccgattgtc
    20641 ggtggttaga aaggcgactt tcattttcaa gtccacattt cacgctcgcg cggataaggg
    20701 gctcagccgg cgccggggca gccgcgcgaa cccccagggg gccaatcatg gatcatcgcc
    20761 ttgcttcggc ccggacaagg ccaagcccgg cttcttcttg cccggctggg atactccgcg
    20821 ggtttggagc gcgcagcaac tcaaatattg agccagccgc gagagaacag gtctccgccg
    20881 accctgactc catcccgccg aaaaaccagg gcacgcgaca acacccgtcc gtccgttccg
    20941 gctttgccga acgttcgcgt cctctccctc ccgccttgga acgtcctttt ac
//
LOCUS       DAOPQG010000064        20750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3164_length_20750_cov_14.707755, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000064 DAOPQG010000000
VERSION     DAOPQG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 20750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20750
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_20750_cov_14.707755"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKI07_08620"
     CDS             <1..335
                     /locus_tag="PKI07_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51154.1"
                     /translation="VQTRNGWKGQNRVHNAIVPPPPTLKSQQAAETAKVKKSKPSAAP
                     ASTAAHKTVSSPSFRAMYKGSTSEARQEHMENKHESPLSLLCTPVVHSLMGALGWLGR
                     VGRVWARA"
     gene            1074..2843
                     /gene="rpoD"
                     /locus_tag="PKI07_08625"
     CDS             1074..2843
                     /gene="rpoD"
                     /locus_tag="PKI07_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOC51155.1"
                     /translation="MISHKARGLTRLPAKTAAARRAAKGHAAPAAAAASAGGSPGAAS
                     PTKHVRAADLTETIKTLLHLAQEHGFVTYDDINDILPDGMSPDDLDQLYMKLRSMDVE
                     IVDHAEVERAKPTEPEEEEDIRLEALDDPVRMYMNQMGKVPLLTREQEVEICKRIEDA
                     EGEIKRLVYCLGFAAKEHNAIAEKLLTEPPKERFDRVVVDKKISNREAHLRELRGLIK
                     AVRVLDKKVDDQFIAWQKTTAQGRRQKLALKLQKLDRKLQDLFPRFFYKQRVLEDMIA
                     VANNVHEKFRGSLRRIQELEARRKSAEQQAGLQAERAKIRALEQFVRLPHEEFFKTLQ
                     ELKRAADRAHRAKTHMAEANLRLVVSVAKKYTNRGQSFLDLIQEGNIGLMKGVEKFEY
                     RRGYKFSTYAIWWIRQAITRSIADQARTIRIPVHMIEIMNKLWRAQKQLTQELGREPT
                     PEEVADEMHMPVSRINALLKMAQQPVSLHAPVGDDGDVNVGDFIEDKTAENPSEVTSY
                     SLLKEKLGDVLTSLTERERKILEMRFGLVDGYERTLEEIGKMYSVTRERIRQIEAKAL
                     RKLRHPTRVRHLQGFLETTEQAA"
     gene            2857..3537
                     /locus_tag="PKI07_08630"
     CDS             2857..3537
                     /locus_tag="PKI07_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOC51156.1"
                     /translation="MKVTYYGHSCFAAQVANRTLLFDPFITGNELARAVEVTKLPADY
                     ILISHGHADHLADAADIARRTGATLVSNYEITLWFGRQGISRTHALNHGGGWRFDFGR
                     VKFVHACHSSSLPDGTYGGNPGGFVVESADGNFYYSGDTALTMDMKLIGESTPLRFAV
                     LCLGDNFTMGVDDALRAAEFVQCREILGCHYNTFPPIQIDTAAARAKFKAAGRNLHLL
                     APGESLNL"
     gene            complement(3562..4170)
                     /locus_tag="PKI07_08635"
     CDS             complement(3562..4170)
                     /locus_tag="PKI07_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51157.1"
                     /translation="MAASSNAPLDLLWKEYSFVFAEFDDLTLARWSVQTLGQLTGKAW
                     RFSHPLVGSYRLAAQLAHDRQVWLKRLVQFPPDYVEAPCCRAPMLPLLTRDVRPEGLI
                     CLHCNATLVPFEEIPAAPRRALGNWADQYASVHAVAHMDDRQRKAVGDYDRAYETAAG
                     DAERLLAQAGWKLAPELLEFYAAVIWEDQDECLEVRPEDVRR"
     gene            4762..5619
                     /locus_tag="PKI07_08640"
     CDS             4762..5619
                     /locus_tag="PKI07_08640"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC51158.1"
                     /translation="MRARKTKTQIRQEQIAAAALELMARHGPKSLNLAALAHQIGVVP
                     SAIYRHYPGKDAVLDAVLDLIASRLQENVNAVRQEESAAPGRLRRLLGLHVHLLQNNM
                     AIPRVVLSEEVFNANTRRRQRVHRIIQDYLGKVAQLVREGQQDGTLRPDVPADTAAVM
                     FLGLIHPAAILWLTSNGAFNVGLHTERAWSLFANMIVAQPAPLAGRLHFATVERRQAV
                     NTSTRDNYENEHRPSPRNRRQSPRRSGVRWSAGRVPGAARTARRAAVTLQSPPQPPRE
                     NGYGSPSPG"
     gene            5800..6972
                     /locus_tag="PKI07_08645"
     CDS             5800..6972
                     /locus_tag="PKI07_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC51159.1"
                     /translation="MKLIALLQHRRARIAFRVLPPALLAIGVAYRAKFAPVPVTAHEV
                     KPTPITAEVMGTGTLEARTKTTLSARLQERLTEVLVDQNDSVKAGQLLARLDDGELTR
                     QVEIATAALAAARATAQRVAVDEARAQAVEKRTRLDHKRLSELVANGIASSADLDKAT
                     EQLQVAEADLKRAHAAIVEAERQVVTAEKSLAFQKERLNYARILSPYDGLVVRRDRDP
                     GGVVVPGSSILQLISTNEFWVSAWVDETASAAILPGQPARVVFRSEPTKTYPGVVARL
                     AREADRETREFLVDVRVTQLPPNWILGQRAEVFVETAHKAGALTVPQPYLHWLEGNAG
                     VFVNDHGKARWRAVTMGLHGRETVEITHGLTPGENVVKPAQAKRRNLLDGQRIARP"
     gene            6969..8180
                     /locus_tag="PKI07_08650"
     CDS             6969..8180
                     /locus_tag="PKI07_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC51160.1"
                     /translation="MNLAAKDIRHNLARFALTTFGIGLLLMIVMGMAGIYRGLVAEAV
                     LLVDKIGADLWLVQGSTRGPFAEISRVPANLEDRARAVPGVASARRFVSHTVQRERHG
                     RPLRIVVQGLAWPEDQGNWVPLVAGRPLRQAHYEMIADRTLGLALGEKVQLGKDVYQV
                     VGLTKGMLGSGGDGLAFFTVSDALAIQFDVPGEATRLERQARHARGEAQDLGRVQPLL
                     LERSLGPATTLPAFTPPQVSAVMVTLQPGADAAKVAATFATWSDVTVYSTQQQIELLL
                     SGMVDKSRRQIGLFSVLLMIISTIIIALIIYTLTLDKIHDIALLKLIGARNSVIVGLI
                     LHEALLIGALAYGLAWWLGQFAFPKFPRLVLIETPDLIALAGIVLGISILASLLGIGK
                     ALRIEPNKVLS"
     gene            8177..8911
                     /locus_tag="PKI07_08655"
     CDS             8177..8911
                     /locus_tag="PKI07_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC51161.1"
                     /translation="MKTNPPAIEAFNLTKIYGHGNTEVVAMNHVSLRVERGEVVALLG
                     PSGAGKSTLLTALGLINPPTSGRIEFSGTLVLEADHPQVDLRSFRRKHLGFVFQKANL
                     IPFLTAVENIQIAMEINDIPPRTARRRALELLDYLGVAPRAGDRPDALSGGQQQRVAV
                     ARALANQPSLILADEPTAALDSHRGRQVMELFRKVAHEQGAGVIVVTHDHRALDVFDR
                     ILEMEDGQIRSESRVPARPKEFPAQN"
     gene            complement(9198..9282)
                     /locus_tag="PKI07_08660"
     tRNA            complement(9198..9282)
                     /locus_tag="PKI07_08660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9246..9248),aa:Leu,seq:tag)
     gene            9599..11392
                     /locus_tag="PKI07_08665"
     CDS             9599..11392
                     /locus_tag="PKI07_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51162.1"
                     /translation="MKRLILALTLVGIVACSMAGFAQTTDSTGATAAAAASTAPAADA
                     APPAASELQRGSIIPLIVMDDVPLTDAIRNLARQAGINYQLDPKIGYGQLGADGKTMA
                     QPNVSIRWENITAEQALTALLGNYDLQLVEDPKSKIARVTAKDPAALPPLLTKIYQLK
                     YCSPSNVLATVQASIDPKRSKVMPDIRTSQLVVVATEQELVAVDELIECLDTKTRQVL
                     IEARLLETIINPKTSKGIDWSGTLKEQNFAFGNGITSGETRQNSSRDSNRSSSRNSTL
                     NANQSRTTTSPGASITTTETLPGGRTITTTTTPESDTTSVVGSSSSSGLSSLASAASG
                     SGLSTLLSSVIGNGGLSLDTAKGFNPATFFLNADGVRATLSFLNEYSETKVISSPRTV
                     TLDNETASIEVGTMWPIVNTTAGTANTTGGSQITYSNLTVMLKVTPRISANNLVRLEV
                     APRVVRLGKSVPSIVGGVQNVVDSFNTREILTSVLIPSGNTLVMGGLMQDEIHNGNTK
                     VPLLGDLPILGLLFRSDTKNREKSNLIVFLTPTIVEDEDYQPTQSDFLKSKVPVSDSI
                     EGDWSAWNSGQPKKWGKKAARPQEPTQEPAL"
     gene            11442..15476
                     /locus_tag="PKI07_08670"
     CDS             11442..15476
                     /locus_tag="PKI07_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51163.1"
                     /translation="MNTALLNFLWRAAGQVFSVRPGARVAALLAFPLSAAHLMGGATP
                     VLVDTLTGGPSQNSPPYHAYVDGNTADLAQFYNPIGLAMDTTGNYLYVADRDNNAVRV
                     LDLPGNYTSTFLPNVFTLPGEINRPVGVAMDAEDNLYVLNRGNGSNGSIKVFDYFGQL
                     IETRATALVNANAIALDAANNAYVTSGNSLIQITTNGVKSTVATITASGASLHGVTVM
                     ESGLVAVCDSGRHGILLINPVTGVVSNHTGFNGVGDNTGVNNRGASRTTATFNRPTGL
                     AKTGGGFLIVADSGNHRVKVVNPIGTVTNLYGVSSNLWVTGPHTWPGWWDGTVWAPDV
                     LGTVEARDPKGVLFSQDGAIYVTEDYYHLIRKATGANLPPFPPPPPTILSVLTNYGQV
                     TLNWSASPGATGYHVKRAPSSAGPYTIIANVTTTSYTDSTGLLDGATYYYVVSALGAG
                     GESANSAPPWAVTLPMPPVPDPIIGYVDFPPTGSATPPTSVFHPVSSHVLNNDTLIVI
                     QGVEGSQTFYTYGPTPASGSIPDPTTDDPSAPSGYQDGLSPIEAAFYRVAEIMPDLTI
                     KAIGTKADGSPNSAIAQARFQFITANPVITGNNAAQFTVSDLTTDALMWYTVDGTEPT
                     NAAPSFGPIGSGTKLSLQFPAGSNTMTFRIRAFRDNYKPSGVVSAIFSITNFQANTIS
                     FGFASGEASSEFIASPGQFFYAPVTLNPLPDTRIYSLQFNVTVGSGGPNPGPAITPGA
                     YNFQSFLEKPDLKNPGYFIRIPPAMFLDSLANPLPVIPTNAVFPYNDGWFEELLFSNT
                     SSNLLGVGWLERAGKTNLYDTTSQDLITYSQPHDTLFRSADGKVVLGGYAFRVPLAAV
                     AGDTYQIQIGRPSATSDGIGAPGHDVYIATPINGSLYGGAINSIKIVTAGQRKYVVGD
                     SAPFRWFNAGDFGNTNTPPLSSVDVLNNSDVMQVFQSAIYHLNYPPPGSDFRDCMDSC
                     GAYGIPASGYWVANTNITDPDVLNQLFDGNDTDINNIAFGDGVLDVCDVYVTFRRSLD
                     PSLTWWQRFWTNGILGATPLSQPKGKGLNGPAVPKASLAFLTNPPSVNFTAGDATTSA
                     GQTVYIPITASIFGDYPLRVLMLNLSVEPLEGSPALGIPVQFTPQAALGAPSISSSIG
                     NGNYAATWLRSDIAGLSGTATLGTLAVTLPAGIYNRAAYAVHFDHASASPNGIASFPS
                     QTRTGLILIGDWSGSIYNDGIPDSWRLRYFGTVNNLLAHADADADGDGAPNWHEYLAS
                     TDPLAAQSVLRVLTDRPLAGESQNCVIRWPGVAGKRYRLERSATLFNPVWTPVYTNTG
                     SGREMQYQDPAGGAVRFYRVTVIP"
     gene            complement(15586..16740)
                     /locus_tag="PKI07_08675"
     CDS             complement(15586..16740)
                     /locus_tag="PKI07_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOC51164.1"
                     /translation="MNLAEILGNEELRHHEFPVTRDAIFLAHAGDCPLPRRVAEAIAD
                     YARQATTGDQEKFVYPAILSRGRQAAARLLNCQPREVAFVGPTSLALSFLASGVKLRR
                     GDNILIYFDDYPSNVYPWMALAEQGVEVRLMNTRGLGAIRPLDVMGQVDENTRLVALA
                     SCHFVSGYRLDFAAIGRQLRERGIRFCLDAIQTLGAFPTTVEQVDMLAADAHKWLLGP
                     CGAGVMYVRDSMQGQVNPPIYGWNNVQCPNFVAQEQLVFREGAQKYEAGTQNLLGIVG
                     LTAAMELLLEVGVENIARELLRKRAWLAPALQAKGCTVVHADAPPEARGGILSFHRPG
                     TDLVALHQKLAEAGVITSLRGDRTGQKYIRVSPHFYNTDAELARLVELIG"
     gene            complement(16815..17492)
                     /locus_tag="PKI07_08680"
     CDS             complement(16815..17492)
                     /locus_tag="PKI07_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOC51165.1"
                     /translation="MKKLTLICLALLSALGVSRAEIVTDSWTQTLSPAVVIPDDNPAG
                     ITFIMPIDWEALHPPSFGASWTFLDVNVILNISGGWNGDLYAHLSYDSVTVPLLNRVG
                     TGNPPNAGEPQFTFGFSTAGFPGITLDDQATANGPIHLIEFPQAGQAYQPDHGTVALN
                     SFCSPLDVNSDGEWTLFLSDLSGNYESSVLYWTVQITAMIPEPSSFSLGLLGLGALLW
                     RRSRSRA"
     gene            complement(17516..20128)
                     /locus_tag="PKI07_08685"
     CDS             complement(17516..20128)
                     /locus_tag="PKI07_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOC51166.1"
                     /translation="MTWMLAVLAMLLFTVPQSLWGYARYWDPDMNVSGNNAGTGAGLG
                     GNGTWSTANANWWNGGAPGAEVAWDNVTRTPRDDAMIWNAGTLTLGSAITARSVRAMG
                     GGNVTLTGTQTLTMGSYNATDVELSAATNYTLTVNSSITIAGTERLYICKNVQGGKVV
                     YTGAGTYSGGLTVWNGTFEFNGDVNAVNNPCGAYPSSFVADNIILTVDAVLRSTKTGA
                     GSSLVKENRGITLTGWATIDIADPTPGNFLIYNGVIAMGANNLVKAGPGMLALSGTTT
                     GNKTIEIHEGALRLRTSHERISNDQRVYLKNSGTLFDVAGYTETIGSLAGEIGSVVDI
                     HGAGSGGAGTLKVNRDNTGTVMSGVIQGAGGTLVKQGTGFLRLAGPSPNTYSGAATII
                     EAGELILDKADGVTAIPRNVTLGDGSGTDQLTLWSHEQIDNSAVVTIAGPSSGNSGKF
                     NLGGYNETLEGISATESFAVIQNTEAPVSAVGTSTLTVSNAGNYTFAGIIRDKASGAN
                     APLALVKANAGTLTLSGANNTYSGITAVNAGTLTLDNGSIGSGSATVESGAILRGKGT
                     LGGDLTIKSGGLLAPGLLTVGQQYGTLTHLGGGNLTLQAGSKTGVDIDRAYAHDQVAG
                     IGTLAEGGTLYVTNRGATLQANDQWPLFVAANYSGTPFAAIEPAQPVSGDPDLVWDKE
                     LLRTNGILRVHHVPFATNLVVVRATNTTWKIPVAKLFPNPDPVDGETVVLESFDHISA
                     NGGRLETYPAYPGLLFYYPANDNNDTFNYTVVDTRGEKRSRTIQVVKGDWYGQGQAPS
                     VPPSGPVTVKFQGVPGLAYEVQRATDVSFSGPLRSWFITPAMNTTGQFQIADDFSDLG
                     SRPSQAYYRLKYNQ"
BASE COUNT         3988 a         7133 c         5900 g         3729 t
ORIGIN      
        1 tggttcagac ccggaacggg tggaaaggcc aaaaccgggt tcacaacgcc atcgttccgc
       61 cgccgccgac cttgaaatct cagcaagcgg ctgaaacggc aaaggttaag aaatcgaagc
      121 cctccgccgc cccggcctcc accgccgccc acaagaccgt ctcttctccg tcctttcgag
      181 ccatgtacaa ggggagtaca tcggaagctc ggcaggagca catggaaaat aagcatgagt
      241 cccctttgag cctcctttgt acacccgttg tacacagcct aatgggagcg ttaggctggc
      301 tggggcgggt ggggcgggtt tgggcgcggg cctgagccgg attggaggaa tggacgagtc
      361 gcgaatgatg taaaaggacg tttcaaggcg ggagggagtg gacgcgaacg ttcggcaaag
      421 ccggaacgag aggccgggtg ttgtcgcgtg ccttggtttt tgggcggtct ggggccacgg
      481 ttggcgggga gatttcttct tccgggcggg ggcggctgct tgcgctgccc ccagggccgg
      541 tcggccgttt tgaagcgata gacgctttta tccccgtccc ctgagcccga aaagcggttt
      601 tgaggttgga aaagccgttt ccccctcagc taccgaaacg caggcgccga taaccaaagg
      661 cccggcagtc tttcccaatt tttctgattt ttctacttgc agaaccgggg ataatttgca
      721 catattaggg attcaaagcc agttttggcc taatgcctgg ggtgaagacc gcccgcggca
      781 cttggagtca ggaagtcgag tttgtcggca gaatagagca ttctgccagt gcgtcagtct
      841 gcgggtgttt gaggtctgta aacactctgc tggacagtcc gagatgaagc cccggagcca
      901 ggccgccgcc gcaaggctgc gtgcaaaaac atctgaaccg ttggaggccg ggcgcgtctt
      961 atagttatat gccgaatcgc agaaagaaaa gaccggccaa aacggtccga ccgcggtgtc
     1021 ctcgtggtaa aaagtccgcc agcgctgccc cgcgccgtcc gcgcccgcgt tttgtgattt
     1081 cccataaagc gcgcggtctg actcgtttgc cggcgaagac cgcggcggcc cggcgcgcgg
     1141 ccaaaggtca tgccgcgccg gcggcggcgg cggcctcggc cggcggcagt ccgggcgccg
     1201 catcgcccac caagcatgtc cgggccgcgg acctgaccga gaccatcaag accttgctgc
     1261 acctggcgca ggagcatggg ttcgtcacct acgacgatat caacgacatt ctccccgatg
     1321 gcatgtcgcc ggatgacctg gaccagcttt atatgaagct gcgctccatg gatgtggaga
     1381 ttgtggacca cgcggaagtg gaacgcgcca agcccaccga acccgaggag gaggaagaca
     1441 ttcgcctgga agcgctggat gacccggtgc ggatgtatat gaaccagatg ggcaaggtgc
     1501 ccctgctgac gcgcgaacag gaagtggaaa tttgcaagcg catcgaggac gccgagggcg
     1561 agatcaagcg cctggtttac tgcctgggct ttgccgccaa agagcataat gccatcgcgg
     1621 aaaagctgct gaccgagcca ccgaaggaac gcttcgaccg cgtggtggtg gacaagaaga
     1681 tttccaaccg ggaagcgcat ttgcgggagc tgcgcgggct gatcaaggcc gtgcgggtgc
     1741 tcgataagaa agtggacgac cagttcatcg cctggcagaa aaccaccgcc cagggccgcc
     1801 gccagaagct ggccctcaag ctgcagaagc tggatcggaa gctgcaggac ctcttccccc
     1861 gctttttcta taaacagcgg gtgctggagg acatgatcgc ggtggccaac aacgtgcatg
     1921 agaaattccg cggcagcctg cggcgcatcc aggagctgga ggcgcggcgc aagtcggccg
     1981 aacagcaggc cggccttcag gcggagcgcg ccaagatacg ggcgctggaa caattcgtgc
     2041 gcctgccgca cgaggagttc ttcaagaccc tgcaggagtt gaagcgcgcc gccgaccgcg
     2101 cgcaccgggc caagacccac atggccgagg ccaacctgcg cctggtcgtg tccgtggcca
     2161 agaaatacac caaccgcggc cagtcgtttt tggatctcat ccaggaaggc aacatcggcc
     2221 tgatgaaagg ggtggagaaa ttcgagtatc gccgcggcta caagttttcc acctacgcca
     2281 tctggtggat ccgccaggcc atcacccgct cgattgccga ccaggcccgc accatccgca
     2341 tcccggtgca catgatcgag atcatgaaca agctctggcg cgcccagaag caactcaccc
     2401 aggaactggg ccgcgagccg accccggaag aggtcgccga tgaaatgcac atgcccgtct
     2461 cccgcatcaa cgcgctgctc aagatggccc agcagcccgt ctcgctgcac gcgcccgtcg
     2521 gcgacgacgg cgacgttaat gtgggcgatt tcatcgaaga caagaccgcc gaaaacccgt
     2581 ccgaggtcac cagctacagc ctcctgaagg agaaactggg cgacgtgctg accagcctga
     2641 ccgaacgcga acggaagatc cttgaaatgc gattcggcct cgtggatggc tacgagcgca
     2701 ccctggagga aatcggcaag atgtacagcg tcacgcgcga gcgcattcgc cagattgaag
     2761 ccaaggcgct gcgcaagctg cgccacccca cgcgcgtccg ccatctccag ggcttcctgg
     2821 aaaccacaga acaagctgcc tgaacccggc cccgccatga aagtcaccta ctacggccat
     2881 tcctgcttcg ccgcgcaagt ggccaaccga accctgctgt tcgacccgtt catcactggc
     2941 aacgaactgg cccgcgcggt ggaggtgacg aaactgccgg cggattatat cctcatttcc
     3001 cacgggcatg ccgatcacct ggcggatgcc gcggacatcg cccggcgaac cggcgccact
     3061 ctcgtctcga attacgaaat caccctctgg ttcgggcggc aggggatttc ccggactcat
     3121 gccttgaacc acggcggcgg ctggcgcttc gattttggcc gcgtcaagtt cgtgcacgcc
     3181 tgccattcca gcagcctgcc ggacggaacc tatggcggca accccggggg ctttgtcgtc
     3241 gagagcgcgg atgggaattt ctactactcc ggcgacacgg cgctgacgat ggacatgaag
     3301 ctgattggcg aatccacccc gctgcggttt gcggtgttgt gcctgggcga taacttcacg
     3361 atgggcgttg acgacgccct ccgggcggcg gaattcgtcc agtgccggga gattctcggc
     3421 tgccattaca acaccttccc tcccatccag attgataccg ccgccgcgcg cgcgaagttt
     3481 aaggcggccg ggcgaaacct gcacttgctg gcgcccggcg agagcctgaa cctttgaggg
     3541 cgggccgggc cggagcgccg gctagcgccg gacatcctcc ggccgcactt cgagacattc
     3601 gtcctggtct tcccaaatga ccgcggcgta gaactccagc agctcgggag ccagtttcca
     3661 gccggcttgc gccagcaggc gctcggcgtc accggcggcg gtctcgtagg cgcggtcata
     3721 atcccccacc gccttgcgct ggcgatcatc catatgggcc acggcgtgga cggacgcata
     3781 ctgatccgcc cagttgccca gggcgcggcg cggtgcggcg gggatttcct caaacgggac
     3841 gagggtggcg ttgcaatgca ggcagatgag cccctccggg cgcacgtcgc gcgtcagcag
     3901 gggcagcatc ggcgcgcggc agcagggggc ttccacataa tcggggggaa actggacgag
     3961 ccgcttgagc cagacttgcc gatcatgcgc caactgtgcg gccagccggt agctgcccac
     4021 cagcggatgc gaaaatcgcc atgccttgcc ggtaagctgg ccaagtgttt gcaccgacca
     4081 gcgcgccagg gtaaggtcat cgaactcggc aaagacgaag ctgtactctt tccagagcag
     4141 atccagcggg gcattgctgc tagcggccat tgaaagctca gtgttgcgct gccggaagag
     4201 attgtcgagc cggaacggcg tcccgcccgc gcccggcggc tggattggcg ggcgcgagag
     4261 ggatcacccc tgaacgcaat tttcgcttac ttgcatttca tccctgtgaa taaaggcaaa
     4321 tttgcatgcc gcccccactg ctcttgcgcg ccagaatgca aggttgcgcc actttcttgt
     4381 ccgcaccgtg ccagcaacgc cacgtcttac accaccggtt gagccaattg cgcccggcgc
     4441 acccgctccg cgatctccgc gccgtcatcc aggcggccgg cttatcgctg agttggtcca
     4501 cctggccgga cctcgtcgcc tcaattgccg ccaaccgcat ctgcgccata agatttcctg
     4561 tctcccgtcg aaacgagttc ttcccagcca ggttccggcc ccagcggcgc cgttgcgccg
     4621 gccccgccgc cggcgttggt ttcaaaccgg tgcggggctt tatccatctg ccgttcgccc
     4681 cacctcaaac ctcgcaaatc ttgacctgaa tcaatactca tccgccgcgc gatgcttata
     4741 ttgtgaacat atattcacaa tatgcgcgcc cgaaaaacaa agacccagat caggcaagag
     4801 cagattgccg ccgccgcgct ggaactgatg gcgcgccacg gtcccaagtc gctcaatctc
     4861 gccgcgctgg cccaccagat tggcgtcgtg ccctccgcca tctaccgcca ctaccccggc
     4921 aaggatgcgg tgctggacgc ggtgctcgac cttatcgcca gccgattgca ggaaaacgtc
     4981 aacgccgtgc gccaggagga atccgccgct cctggccgtt tgcgtcgctt gctcggtctt
     5041 cacgtccacc tgctccagaa caacatggcc atcccccgcg tcgtgctctc ggaagaagtc
     5101 ttcaatgcca acacccgccg caggcaacgc gtccatcgca tcattcagga ctacctcggc
     5161 aaagtggcgc aactcgtccg ggaaggccag caggacggca ccctgcggcc tgacgtgccg
     5221 gccgacacag ccgccgtcat gttcctcggc ctcatccacc ccgccgccat cctctggctc
     5281 accagcaacg gcgctttcaa cgtcggcctc cacaccgaac gcgcctggag cctgttcgcg
     5341 aacatgatcg tggcacaacc tgctcctttg gccgggcggc tccattttgc gacggtagaa
     5401 cgccgtcagg ccgtaaacac atcaacacgc gataattatg aaaacgaaca ccgaccgtct
     5461 ccgcgcaacc gtcgccaaag ccctcgccgc tcaggcgtcc gctggtccgc aggacgcgtc
     5521 cccggcgccg caagaactgc ccgccgcgct gcagtcaccc ttcaatctcc tccccaacca
     5581 ccgcgggaaa acggatatgg gagtccctcc cccggctgac agcgtttcca ccgacaccga
     5641 cccgcaggaa cgctgcgata gattcgccgc aggggcttac gccgtcggcc tcgatgttcg
     5701 cgcccggctg gagaagttct ccaaaggcct caaaccatcc ccccgggcac ccctggccgc
     5761 cgcggttcct gcgggcaggc gcgcatgcca aggtaaaaca tgaaactgat tgcgctcctc
     5821 cagcaccgcc gcgccaggat agccttccgg gtcctgccgc ccgcgctgct tgccatcggc
     5881 gtcgcctacc gcgccaagtt cgcgccagtg ccggtgacgg ctcacgaggt caagcccacc
     5941 cccatcaccg ccgaggtgat gggcaccggc acgctggagg cccgcaccaa aaccaccctc
     6001 agcgcccgcc tccaggagcg cctgaccgaa gtcctcgtgg accagaacga ctctgttaaa
     6061 gccggccagt tgctcgcgcg cctcgacgac ggcgaactga cccggcaggt cgaaatcgct
     6121 accgctgcgc tcgcggcagc ccgcgccacg gcgcagcggg tagccgtgga cgaagcccgc
     6181 gcgcaagcgg tcgaaaagcg gacccggctc gaccacaaac gcctctccga gctggttgcc
     6241 aacggaatcg cttcgtcggc ggatctcgat aaagccaccg agcagttgca agtggccgaa
     6301 gccgacctga agcgcgccca cgccgctatc gtcgaagcgg agcgccaggt cgtgaccgcc
     6361 gagaagagcc tcgccttcca aaaagagcgc ctcaactacg cgcggattct cagtccgtac
     6421 gacggcctgg tcgtgcgccg cgaccgcgac cccgggggcg tagtcgtgcc gggcagttcc
     6481 atcctccaac tgatttcgac caacgaattc tgggtgtccg cctgggtgga tgaaacggct
     6541 tccgcggcca tcctccccgg ccagccggcg cgcgtggtct tccgttccga gccgacaaag
     6601 acttaccccg gcgtcgtcgc ccgcctcgcc cgcgaagcgg accgcgaaac ccgcgagttc
     6661 ctcgtggatg tgcgggtgac gcaactcccc cccaactgga ttctcggcca gcgcgccgaa
     6721 gtattcgtcg aaactgcgca caaagccggc gccctcaccg tgccccagcc gtacctccat
     6781 tggcttgaag gcaacgccgg tgtgttcgtg aacgaccacg gcaaagcccg ttggcgagcc
     6841 gtgacgatgg gcctgcacgg acgcgagacc gtcgaaatca cccacggtct cacccccggg
     6901 gagaacgttg tgaagccggc ccaggcgaag cggcgaaacc ttcttgacgg gcaaagaatc
     6961 gcaaggccat gaatctcgcc gccaaagata tccgccacaa cctcgcgcgc ttcgctttga
     7021 ccacgttcgg gatcggcctg ctcctcatga ttgtaatggg gatggccggc atctaccgcg
     7081 gcctcgtcgc cgaggccgtg ctcttggtgg ataaaatcgg cgccgacctg tggctcgtcc
     7141 agggcagcac ccgggggccg tttgcggaaa tctcacgcgt gcccgcaaac ctcgaagacc
     7201 gcgcccgcgc agtgcccggc gtcgccagcg cccgccgctt tgtctcccac accgtccagc
     7261 gcgaacgcca cggacgcccg ctgcgcatcg tcgtccaggg actggcctgg cccgaggacc
     7321 agggcaactg ggttcccctt gtcgctggcc gcccgctgcg ccaggcgcac tacgagatga
     7381 tcgccgatcg cacgcttggt ttggcgcttg gcgaaaaggt ccaactcggc aaggatgtct
     7441 atcaagtcgt cggcctcacc aaaggcatgc tcggctccgg cggagacggg ctcgccttct
     7501 tcaccgtaag cgatgctctg gccatccaat tcgacgtgcc cggcgaagcc acccggctgg
     7561 agcgccaggc ccgccacgct cgtggcgaag cgcaagacct cggccgggtg caaccgctct
     7621 tgctggagcg cagcctcggc cccgcaacaa ccctcccggc gttcacgccg ccccaggtca
     7681 gcgcggtcat ggtcaccctg caacccggcg cggatgccgc gaaagtcgcc gccaccttcg
     7741 ccacttggtc cgacgtcacg gtctattcga ctcagcaaca aatagaactg ctcctttccg
     7801 gcatggtgga caaatcacgc cgccagatcg gcctctttag cgtgctcctg atgatcattt
     7861 ccaccatcat catcgccttg atcatctaca cattgacttt ggataagatt cacgacatcg
     7921 ccctccttaa gctcatcggc gcgcgcaata gcgtgatcgt ggggttgatt ctccacgagg
     7981 cgctgctcat cggcgccctt gcctacgggt tggcctggtg gctggggcag tttgcctttc
     8041 cgaagttccc gcgcctggtg ctcattgaga ctcctgactt gatcgccctc gctggcattg
     8101 ttctgggcat ttccattctg gccagtttgc tcggcatcgg caaggccctg cgcatcgaac
     8161 ccaacaaggt gctctcatga aaacaaaccc tcccgccatc gaggccttca acctcaccaa
     8221 gatctacggc cacggcaaca ccgaggtcgt ggccatgaac catgtttcgc tgcgcgtcga
     8281 acgcggcgaa gtcgtcgctc tgctcggccc tagcggagcc ggcaaatcca ctctcctcac
     8341 cgccctcggc ctcatcaatc cgccgacgtc cggccgcatc gagttcagcg gcaccctggt
     8401 gttggaggcg gaccaccccc aggtggacct gcgctccttc cggcgcaaac acctcggctt
     8461 cgtgttccaa aaagcgaacc tcattccatt ccttaccgcg gtcgagaaca tccagattgc
     8521 gatggagatt aatgacatcc ccccacgaac cgcccgccgc cgcgccctgg aactgctcga
     8581 ctacctcggc gtcgccccgc gcgccggcga ccgccctgac gccctctccg gcggccagca
     8641 acagcgcgtc gccgtcgccc gcgcgctggc caaccagccc agcctcatcc ttgccgacga
     8701 acccactgcc gccctcgata gccatcgcgg tcggcaggtc atggaactct tccgcaaagt
     8761 cgcccatgag caaggggcgg gcgtcatcgt cgtcacccat gaccaccgcg cgctggacgt
     8821 ctttgaccgc atcctggaga tggaggatgg ccaaatccgc tccgaatcca gagttcccgc
     8881 ccgcccaaag gagttcccgg cacaaaactg acctcaccgc aaaagataaa ccccgaccgg
     8941 agcgagagcc ctgctctcca cacctcttgc aaacccaagg ccctcccccc ctgccggcat
     9001 gaccgtgccg tcctgactga cctcggtacg gacggtggcg cgggtatccg gcagcgccaa
     9061 ttccgcgcgc acgctgccgt tggggccccc cgcttcacct gtggttttcc tgctgcaacg
     9121 ccggtatgtt tcatatcggc gattgtgcca gcaagcggcc atcacatcac aaccaccaac
     9181 aaaatccttg gttctgttgg tgggcgcgac agggatcgaa cctgcaagga ttgctccaaa
     9241 ggatcctaag tccttcgcgt ctgccaattc cgccacgcgc cctggaggcc caaacatctt
     9301 cagcacagcc gaagcccttg taaatatctt tcctcgctgg tggcggaaac cgcaggccac
     9361 gggcatgggg atagccggga gaacgggcgt cctccgggag gcaaagccgg aatccagcgc
     9421 cgaaccgggc gtttttgtcc tgataccgga cactaattcc gccaagtggt cacttttcgg
     9481 ggtttgagac aaaaagcaac cccggtttat ctctttggca ccctgactgc tcaatagctc
     9541 cccgaatgag acggaaaagt ctgtccaggt ccggttacac gcagaactga tttaaaacat
     9601 gaaacgtcta atcttagctc tcacgctcgt tgggatcgtc gcctgcagca tggctggctt
     9661 tgcccaaacc accgattcaa ctggtgcgac ggcggctgcc gctgccagca ctgcccccgc
     9721 cgctgacgcc gctccgccgg ctgccagcga actccaacgc ggcagcatca ttcccctgat
     9781 tgtgatggat gatgtgccgc tcaccgatgc catccgcaac ctcgcgcgcc aggccggcat
     9841 caactaccag ctcgatccca agattggcta cggccaattg ggagcggacg gcaagacgat
     9901 ggcacagcca aacgtcagca tacgctggga aaacatcacc gcggaacagg ccctcaccgc
     9961 cctgctcggc aactacgacc ttcaattagt ggaagatccc aagagcaaaa tcgcccgcgt
    10021 cacggcgaaa gacccggccg ccctgcctcc gctgctcacc aagatttatc aattgaaata
    10081 ctgcagtcca tccaacgtgc tggccacggt gcaagccagc attgacccca agcgcagcaa
    10141 agtgatgccg gacatccgca ccagccagtt ggtggtcgtg gccaccgagc aggaactggt
    10201 cgccgtggac gaattgatcg agtgcctgga caccaagacc cggcaggttc tcatcgaagc
    10261 gcgcttgctg gaaaccatca tcaaccccaa gacctcgaag ggaattgact ggtccgggac
    10321 gctcaaggaa cagaacttcg ccttcggcaa tggcatcacg agcggcgaaa ccaggcaaaa
    10381 ctcctcccgg gattccaacc ggtcctcatc ccgaaactcc accctgaacg ccaaccaaag
    10441 ccggacaacc acctcgcccg gagcttccat caccaccacc gagaccctgc ccggcggacg
    10501 caccatcacc accaccacca cccctgaatc cgacaccacg tcagttgtgg gcagctccag
    10561 cagttccggg ctcagctcgc tggccagcgc ggccagcggt tccgggctca gcacgttgct
    10621 gagctcggtc atcggcaacg gcggcctgtc gctggatacc gccaaaggct ttaatcccgc
    10681 caccttcttc ctcaatgcgg atggcgtccg ggcgacgttg tcgttcctta acgaatattc
    10741 cgagaccaag gtaatctcct cgccgagaac ggtcaccctg gacaatgaaa ccgccagcat
    10801 tgaagtgggc accatgtggc cgatcgtcaa tacgaccgcc ggcaccgcca acaccaccgg
    10861 tggatcgcaa atcacctact ccaacctcac ggtcatgctc aaggtcaccc cccggatttc
    10921 cgccaataac ctggtccgcc tggaagtcgc gccgcgggtg gtccggctgg gcaagtccgt
    10981 gccgagtatt gtgggcggag tccaaaacgt cgtggattcc tttaacactc gcgagatcct
    11041 gacctccgtg ctcattccca gcggcaacac gctggtgatg ggtggcctga tgcaggacga
    11101 aatccacaat ggcaacacca aggttcccct gctcggcgac ctcccgatcc tgggattgct
    11161 cttccgcagc gacaccaaaa accgcgaaaa gtccaacctc atcgtcttcc tcaccccgac
    11221 catcgtcgag gacgaggact accagccaac ccaaagcgac ttcctgaaat ccaaagtgcc
    11281 ggtctcggac tccatcgagg gtgactggtc tgcctggaac agcggccagc ccaagaaatg
    11341 gggcaagaag gcggcgcggc cccaggagcc gactcaggaa cccgctctat aacgcaactt
    11401 agacgcattc gcggtcatac catgactcct atacgcaagc tatgaatact gcattactca
    11461 atttcctctg gcgggcggcg gggcaggtct tctccgtccg gcccggcgct cgggtcgccg
    11521 cactgctggc cttcccgcta agtgccgctc atctcatggg gggagccacc ccggtcctgg
    11581 tggataccct caccggcggg ccctcgcaaa acagccctcc ctaccatgcc tacgttgatg
    11641 gaaacaccgc cgacctggcg caattctata atcccatcgg cctcgccatg gacaccaccg
    11701 gcaattatct ttacgttgcc gaccgcgata ataacgcggt gcgcgtgctt gatctgccgg
    11761 gcaattatac gtcaactttc ctccccaatg tgttcaccct gccgggagaa attaaccgcc
    11821 cggtcggcgt ggccatggat gccgaggaca acctctatgt gctcaaccgg ggcaatggca
    11881 gcaacggctc gataaaagtc ttcgactact tcggccaact gatcgagacc cgcgccaccg
    11941 cgctggtcaa tgccaacgcc atcgccctgg acgcggccaa taatgcctac gtgacctccg
    12001 gcaactcgct aatccagatc acgacgaacg gcgttaagag caccgtcgcc accatcacgg
    12061 cttccggcgc ctcgctgcac ggggtgaccg tcatggaaag cggcctggtg gccgtttgcg
    12121 actccggccg ccacggcatc ctcctcatta acccggtcac gggggtcgtt tccaaccaca
    12181 ccggctttaa cggggtgggg gacaacacgg gcgtgaacaa ccgcggggct tcccgaacca
    12241 ccgccacctt caatcggccc acaggcctgg ccaagaccgg cgggggcttc ctgattgtcg
    12301 ccgattccgg caaccaccgg gtcaaagtcg tgaaccccat cggcaccgtc accaatctct
    12361 acggcgtcag ctccaatcta tgggtgaccg gcccgcatac ctggcccggc tggtgggacg
    12421 gcacggtctg ggcgccggat gtcctgggca cggtcgaagc ccgcgacccc aagggcgttc
    12481 tgttcagcca ggacggcgca atctatgtca ccgaagacta ttatcatttg atccgcaagg
    12541 ccacgggagc caaccttcct cctttcccgc cgccgccacc gaccatcctg tcggttctga
    12601 cgaactacgg acaagtcacg ttgaactggt ccgcgtctcc tggcgcgacc ggctaccatg
    12661 tgaagcgggc gccttccagt gccggccctt acaccatcat cgccaacgtc acgacaacga
    12721 gctacacgga ctccaccggc ctcctcgacg gggcgaccta ttattatgtc gtgtccgctc
    12781 tcggtgcggg aggcgaaagc gccaactccg ccccgccgtg ggccgtcacc ctgcccatgc
    12841 cgccagtgcc cgatcccata atcgggtatg tggacttccc cccgacgggg tccgcgacgc
    12901 caccgacctc ggtgtttcat cccgtctcct cccatgtcct caacaatgat acgctcattg
    12961 tcattcaagg agtcgaaggc agccagacct tctatactta cggccctacg ccggcttccg
    13021 gcagcatccc ggatcctacg acagacgacc cctccgcccc gtccggctac caggatggcc
    13081 tgtcacccat cgaggcagcc ttttatcgcg tggccgagat catgcccgat ttaaccatca
    13141 aagccatcgg cactaaagcg gatggcagtc cgaacagcgc cattgcgcag gcccggttcc
    13201 agttcattac ggccaatccc gtcatcacgg gcaacaacgc ggcacaattc accgtgtccg
    13261 atcttaccac cgacgccctc atgtggtata ccgtggatgg aacggagcct accaatgccg
    13321 cgcccagttt cgggcctatc ggcagcggca ccaagctctc gctccaattc cccgcgggca
    13381 gcaacacgat gacgttccga atccgggcct tccgcgacaa ttacaagccc agcggcgtgg
    13441 tctctgccat cttttcgatt accaacttcc aggctaacac aatcagcttc ggcttcgcca
    13501 gcggcgaggc ctccagcgaa ttcattgcct cgccgggcca gttcttctat gccccggtga
    13561 cgctgaatcc tctccccgat acgcgaattt atagcctcca gttcaatgtg accgttggca
    13621 gtggcggccc caatcccggg ccggcaataa cgcccggcgc ctataatttc cagtccttcc
    13681 tggaaaaacc agacctcaag aatccgggat atttcatcag gatccccccg gcgatgttcc
    13741 ttgacagcct cgccaaccct ctaccggtta tccccacgaa tgcggtcttc ccctacaacg
    13801 atggctggtt cgaagaactc ctcttcagca acaccagcag caatctgctc ggcgtaggct
    13861 ggctggagcg cgccggcaag acaaaccttt atgataccac gtctcaggat ttaatcacct
    13921 actctcagcc ccacgacacg ctctttcgct cggcggatgg caaagtcgtc ctcggcggct
    13981 acgccttccg agttcccctc gccgccgttg cgggcgacac ttaccaaatc caaatagggc
    14041 ggccttcggc gacgtccgac ggcatcggcg ctccagggca cgatgtctat atcgcaacgc
    14101 ccataaatgg ctcgctctac ggaggcgcca tcaattccat caagattgtc accgcgggcc
    14161 agcgcaagta cgtggtcggc gatagcgccc cgttccggtg gtttaatgcg ggtgattttg
    14221 gcaacaccaa tacaccgccc ctcagcagcg ttgacgtcct caacaacagc gacgtcatgc
    14281 aggtattcca gtccgccatc taccacctga actacccgcc gccgggcagc gacttccggg
    14341 actgcatgga ttcctgcggc gcttacggca tccccgccag cggctactgg gtggccaata
    14401 cgaacatcac cgacccggat gtcctgaatc aactgtttga tggcaacgat accgacatca
    14461 ataatattgc ctttggcgat ggagtgctgg atgtgtgtga cgtttacgtc accttccggc
    14521 gctccctgga cccgagcctg acctggtggc agcggttctg gaccaacggc atcctgggcg
    14581 ccacgccgct ctcccagccc aagggcaagg gcctgaatgg ccccgcggtg cccaaagcct
    14641 ccctggcatt cctgacgaat ccgccgtctg tgaacttcac cgccggagac gccacaacct
    14701 ccgccgggca gacggtctat atccccatca cggccagcat cttcggcgat tacccgttgc
    14761 gcgtgctcat gctgaacctg agcgtggaac cgctggaagg ctcgcccgcc ctgggcatcc
    14821 ccgttcagtt cactccccag gcggccctgg gcgcgccgtc catatcttcc tcgattggca
    14881 acggcaatta tgccgccacc tggctccgga gcgacattgc cgggctgagc ggcaccgcca
    14941 ccctgggcac cctggccgtc acgctgcccg cgggcatcta taatcgcgcc gcctacgccg
    15001 tccatttcga ccatgcgtcg gcttcgccca acggcatcgc ttcgttcccg agccagaccc
    15061 gcaccggcct gatcctgatc ggcgactggt ccggctccat ctataacgac ggcatccccg
    15121 attcctggcg cctccgctac ttcggcaccg tcaacaacct cctcgcgcac gcggacgccg
    15181 atgccgatgg cgacggcgct cccaactggc atgagtacct ggccagcacc gatcccctcg
    15241 ccgcccagtc ggtgctgcgc gtcctcaccg accggccgct cgccggggaa agccagaatt
    15301 gcgtaatccg ctggcccggc gtagccggca agcgctatcg cctcgaacgc tcggccaccc
    15361 tcttcaaccc ggtctggact ccggtttaca ccaacaccgg cagcggccgg gaaatgcagt
    15421 accaggatcc ggccgggggc gccgtgcgct tctaccgggt gactgtgatc ccataacgcg
    15481 gcggtcattc ccgccggtcg cttcacgcgg cgcattcctc aagaatgcgc cgcttttgcg
    15541 tcggcgggcg ttccggcgct cccttcccgg acccgcgccg gccgatcagc caatcaactc
    15601 caccagccgc gccagctccg cgtcggtgtt ataaaaatgc ggcgacacgc ggatgtattt
    15661 ctgtcccgtc cggtcgccgc gcagggatgt gatcaccccg gcctcggcga gcttttggtg
    15721 cagcgcaacg agatcggtgc cggggcggtg aaaggagagg atgccgccgc gggcttccgg
    15781 gggcgcgtcg gcatgcacga ccgtgcagcc tttggcctgc aaggccggcg ccagccaggc
    15841 ccgcttgcgc aacaactccc gcgcaatatt ctccaccccc acctccagca acagctccat
    15901 ggccgccgtc agcccgacga tccccagcag gttctgcgtc cccgcttcat atttctgcgc
    15961 gccctcccgg aataccagct gctcctgggc gacgaagttc gggcactgca cattattcca
    16021 gccatagatg ggcgggttga cctgcccctg catggaatcg cgcacgtaca tgacccccgc
    16081 gccgcaaggc cccagcagcc atttatgcgc gtccgccgcc agcatatcca cctgctcgac
    16141 cgtcgtggga aacgccccca gcgtctgaat ggcgtccagg cagaaccgga tgccgcgctc
    16201 gcgcaactgc ctgccaatcg cggcaaaatc cagccggtag ccgctgacga aatgacagga
    16261 agccagggcg accaggcgcg tgttttcgtc cacctgcccc atcacgtcca gcgggcggat
    16321 ggcccccagc ccgcgggtat tcatcaggcg cacctccacg ccctgctccg ccagcgccat
    16381 ccacggatac acgttggatg ggtaatcatc aaagtagatc aggatgttat ccccgcgccg
    16441 cagcttgacg ccgctggcca ggaaactcaa cgccagggag gtcggcccca caaaggccac
    16501 ttcccggggc tggcagttga gcagccgcgc cgcggcctgc cgtccccggc tcaggatggc
    16561 gggatataca aacttctcct ggtcgccggt ggtggcctgc cgggcatagt ccgcgatcgc
    16621 ctcggccacg cgccgcggca gcgggcaatc ccccgcgtgc gcgaggaaga ttgcgtcacg
    16681 ggtaacggga aattcgtggt ggcgcagctc ttcgttgccc aggatttcag ccaggttcat
    16741 cccccgaagc taatccttta ttctcccgcc gcaagggcgg aatgcgctcc cgccgcgggc
    16801 aacgcggcgg ccgctcaggc gcggctgcgg gagcggcgcc acagcaacgc ccccagcccc
    16861 agcaaaccca gcgagaagct cgacggctcg ggaatcatcg cggtgatctg caccgtccaa
    16921 tacaacaccg agctctcata gttgccggac aaatcgctca gaaacagcgt ccactccccg
    16981 tccgagttca catccagggg gctgcaaaag ctgttcagcg ccacggttcc gtggtccggc
    17041 tgatacgcct ggccggcctg gggaaactcg atcaaatgaa tggggccgtt ggcggtggcc
    17101 tggtcgtcga gggtgatgcc ggggaatccc gcggtcgaga acccaaaggt gaactgcggc
    17161 tcgccggcgt tcggcggatt gcccgtgccc acccggttca gcaggggcac cgtcacgctg
    17221 tcatacgaga gatgggcgta gagatcccca ttccagccgc cggagatgtt gaggatcaca
    17281 ttgacgtcca ggaatgtcca actggcgccg aaactgggag gatgcaacgc ttcccaatca
    17341 atgggcatga tgaacgtaat gcccgcgggg ttgtcgtcgg ggatgaccac ggccgggctc
    17401 agggtctggg tccaggaatc cgtgacgatc tcagcccgtg acaccccgag cgcgctcagc
    17461 agcgccaggc agattaaagt cagcttcttc atcgtaaaat cctcgtgctc cgtaattact
    17521 ggttgtactt caagcgatag taggcctggc tgggccggct gcccaggtcg ctgaagtcat
    17581 ccgcgatttg gaattggccc gtcgtgttca tcgccggcgt gataaaccaa ctgcgcagcg
    17641 gcccgctgaa actcacgtcc gtggcgcgct ggacttcgta ggccaggccc ggcacgccct
    17701 ggaacttgac cgtgaccggc ccgctcggcg gcaccgaggg agcctgcccc tgcccgtacc
    17761 agtcgccctt cacgacctgg atcgtccggc tgcgcttctc gccgcgggta tccacgaccg
    17821 tgtagttaaa cgtgtcattg ttgtcattgg ccgggtagta gaagagcagc cccggatacg
    17881 cgggataggt ctcgagccgg ccgccgttgg ccgaaatatg gtcaaagctc tccagcacca
    17941 ccgtctcgcc gtcaaccgga tcgggattgg ggaacagctt ggccaccgga atcttccagg
    18001 tggtattggt ggcgcgcacc acgaccaggt tggtcgcgaa cggcacgtgg tgcacccgca
    18061 ggattccatt ggtgcggagc agctctttat cccagaccag gtccgggtcg ccgctcaccg
    18121 gctgcgccgg ctcaatcgcc gcaaacggcg tgccgctgta gttggcggcg acaaagagcg
    18181 gccattgatc attggcctgc agcgtcgcgc cccggttggt gacatacagc gtgccgccct
    18241 cggccagcgt gccgatgccg gcgacctggt cgtgggcgta ggcgcggtca atgtccaccc
    18301 cggtcttgct cccggcctgc agggtcagat tgcccccgcc caggtgggtg agcgtgccgt
    18361 actgctggcc gaccgtcagc agccccggcg ccagcaaccc gccgctcttg atagtcaaat
    18421 cgccgccgag ggtgcccttg ccgcgcagga ttgcgccgct ctccacagtg gcgctgccgg
    18481 agccgatcga gccgttgtcc agggtcagcg tgcccgcgtt gaccgcggtg atgccgctgt
    18541 aggtattatt ggcgccgctg agcgtcagcg tgccggcatt ggccttgacc agcgccagcg
    18601 gagcattggc gccgctggcc ttgtcccgga ttatccccgc aaacgtatag ttgcccgcat
    18661 tgctcaccgt cagcgtcgaa gtgccgacgg cgctgacggg cgcctcggtg ttttggatga
    18721 ccgcaaagct ttccgtggcg ctaatcccct ccagggtttc attatagccg cccaggttga
    18781 acttgccgct gttgccggac gaggggccgg cgatggtcac caccgcgctg ttatcaatct
    18841 gctcgtggct ccagagcgtg agttgatccg tccccgagcc atcccccagg gtcacgttcc
    18901 tcggaatcgc ggtcaccccg tccgccttgt ccaaaataag ctcgcccgcc tcgatgatcg
    18961 tcgcggctcc cgagtaggtg ttgggcgacg gccccgccag ccgcaggaag cccgtgcctt
    19021 gcttgaccag ggtcccgccg gctccctgga tcacccccga cataacggtg cccgtattgt
    19081 cccggttgac tttcaaagtg cccgcgccgc cgctgccggc cccgtggatg tccaccacgc
    19141 tgccgatttc gcccgccagc gacccgatgg tttcggtata gcccgccacg tcgaacagcg
    19201 tgccgctgtt cttcaggtaa acccgctggt cattcgagat gcgctcgtgg ctggttcgca
    19261 gccgcagcgc gccttcgtgg atttcaatcg tcttgttgcc ggtggtggtt ccgctcaggg
    19321 ccagcatgcc gggccccgcc ttgacgagat tgttggcccc catggcgatg acgccgttgt
    19381 aaatcaggaa attgccgggc gtggggtccg caatgtcaat ggtggcccag ccggtaagcg
    19441 tgatgccgcg attctctttg accagcgaag agccggcgcc cgtcttggtc gaacgcagca
    19501 cggcgtctac ggtcaggatg atgttatccg caacgaagct ggaggggtaa gccccgcagg
    19561 gattgttgac cgcattcaca tcgccattga actcgaacgt tccgttccaa accgtcaggc
    19621 cgccgctgta agtgcccgcc ccggtataaa ccaccttgcc cccctggacg ttcttgcaga
    19681 tatacagccg ttccgtcccg gcaatcgtga tggatgaatt caccgtcagg gtataattgg
    19741 tcgccgcgct gagttcgaca tcggtggcgt tgtacgagcc catggtcagg gtctgggtgc
    19801 cggtcagcgt gacattcccg cctcccatcg cccgaactga gcgcgcggtg atcgcggagc
    19861 ccagcgtgag ggttccggca ttccaaatca tggcatcatc ccgcggagtg cgcgtgacgt
    19921 tgtcccaggc aacctccgct cccggggccc ctccattcca ccagtttgca ttcgccgtac
    19981 tccacgtgcc gttgccgccc aatcccgcac ccgtgcccgc gttgttgccg ctgacattca
    20041 tatcgggatc ccaatatcgg gcataacccc acaggctctg gggaacggta aacagcagca
    20101 tggccagcac ggccaacatc caagtcattc tcggcacgtt catcgcgcgc ccatgcgcgc
    20161 tcgctagaaa tcgcttcatg gtggaatcgt ctaaaactta tcgccaataa acttcagact
    20221 accatttttc acatttgcac attgttgtca agaatttagt caaaaaatca tctgcggcac
    20281 gaccgcaatt ccgggattta acctttctcc gcccgacaga gagactgctc ctgaaataag
    20341 catccgaaaa ctcgaaatcg cgaaatgact cgctcccctg ccccggggtt atcaaatacc
    20401 tcgcccccac ccataacggc cataaaccgc aagtagctgt atccacgctg cttatcaacc
    20461 atttcccgct gtggatttcg cgttcttgct ttccacgccc cccactactc cccacttgca
    20521 cctcaacgca ccctgatgcc ttgcctttaa ccgggccgac tcggatttct ccccccattc
    20581 aaccccacga aacgcgcaag tccctggctt tacgggtgga aggcattcgg agtccagcaa
    20641 tccgcccatt gatttcacca atccagcagt tcgttgatcc tctccgccgg cctgccaagt
    20701 tgtgtccgtc aagttcagca aattggttat catggtttcc actcaaggtg
//
LOCUS       DAOPQG010000065        20267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3266_length_20267_cov_16.375272, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000065 DAOPQG010000000
VERSION     DAOPQG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 20267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20267
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3266_length_20267_cov_16.375272"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..965)
                     /locus_tag="PKI07_08690"
     CDS             complement(156..965)
                     /locus_tag="PKI07_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOC51167.1"
                     /translation="MASKSLSCSSPAIGLPSEGCAAADPYRPSAIPKPSKDVFIVVLS
                     CVLSAVRRAYTNFSPPEQSEKLPPRARARLARPGLAPGCPPAAAGPPPKPPRFQARLR
                     RQPSNAPRESRHSVFKHPTPPGTGRPAGTGQPACQNSARPNRQPESQSTSPAPAVPSR
                     PPSQADYLKEELSLLWEPPSRVAMEDFLRQWCARAMETGIALLRQMAKTLRLHARGIL
                     SWWEHRLSSGKMEGVNNKIKTLTRQAYGYRNEAFFILKLLALHHARIKLVG"
     gene            847..2370
                     /locus_tag="PKI07_08695"
     CDS             847..2370
                     /locus_tag="PKI07_08695"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOC51168.1"
                     /translation="MKTSLLGFGIALGLYGSAAAQPSLGSPMAGLEQLKDFEAMRESS
                     SDPDWHNGNADSRPIEPGGTLVLADLKGPGVITHFWNTIAHPAPFYPRLLTLRMYWDG
                     EEHPSVECPIGDFFGIGHGLDKSFVSLPIKVSSDGRGRNCYWPMPFRKSARITVSNES
                     DRRCDAFYYYLDWQKHKSLPRNTAYFHAMYRQEHPCVMGRNYLLADLQGRGHYVGTVQ
                     SVQNMSAGWYGEGDDFFFIDGETEPRLRGTGTEDYFCDGWGFRPHDGPFYGVPLWEGY
                     APGNRGSVYRFHLTDPVPFKKSLRVEIEHKGSQEFPDRSVSGFIERDDLMSSVAFWYQ
                     VEPHKPWPALPPGPERLAEQTLALVTGWKAVGGAKHSDHPLAVQDLPGIAKEGKQLFF
                     TPRNDQGWVECPFQVGKDIRAHLWGKFIQSHDYGIYRVTLDGRELAVVNLYHPETRRH
                     VYYWGPQALSAGAHVLRFECQGKADHSTGYCLGLDSIAAVVPAYERPPGFDLRKIQR"
     gene            2487..3713
                     /locus_tag="PKI07_08700"
     CDS             2487..3713
                     /locus_tag="PKI07_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOC51169.1"
                     /translation="MNSDLRFTGKGTAKLAGAVLAALAWPQALLSAAEPAAWLTFGLD
                     RVPGLGYEVLGNPLWQYLAFLIYVALAFYASKLLDALIQHRLQKWAARTQTKLDDLLL
                     ELLRGPIKVVAFVVFLHIGLRLFNWPVWASKFISNGLKIVVACSLTYIAIKFVDLLAG
                     LWHKQMAAHDDLADHQLFPIIRKTLKIFVVVVAVLVTSQNLGMNVTGLLASLSIGGLA
                     VGLAAQDALSNFFGAVAIFADKPFRIGDRIQLDSVDGSVEAIGLRSTRVRNLDGHLVT
                     IPNRTMANANIVNVSRRPNIKTVMNIGLTYDTPAAQVERAVQIIEEIFRPHPRTVDLL
                     ISFNKFEASSLNVLVVHRWGSTDYPEYLRGFQQLNLELKRRFDAEGISFAFPTQTIYV
                     RPDPGAPPAGAPVAAR"
     gene            complement(3735..7079)
                     /locus_tag="PKI07_08705"
     CDS             complement(3735..7079)
                     /locus_tag="PKI07_08705"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOC51170.1"
                     /translation="MPDLVQLITAADPARRNQALAAACAGLSAGDLLDQCARLDDFRR
                     RSENLYERVRALFFLYAIHRFHLPERLASAAAPAGRRSLIPFAGYEHLLQRRFEEALD
                     QFLAVQQAEGPSDAISSALAAAYHRLGFQTLADQVRRSVRSVRGNQWMFRMGHPADHP
                     LRLRPELCQPHPGDGTYPILRECTPVRMDLTHCGWSDIFFLGMDYPEGAKVLNVSIDL
                     GVHGRDPAPRPPVEASLRVIEEPLLRLASLDLGAAADITNLAEVFDFAKDYLGLLKAA
                     VIAAGLVPPGIEGSGQSLAELLARLAGRGRGLEIVSRVNNIPKGSRLAVSTTLLAALV
                     SVCMRATGQAASLTGPLQEHERRLVLARALLGEWLGGSGGGWQDSGGVWPGMKLIEGV
                     PAGEGDPEFGVSRGRLMPRHRILAAADAALETRRRLQESLVLVHGGMAQNVGPILEMV
                     TEKYLLRCADEWQARQSMLGILDEILAALRAGDVRRIGAATTRNFQQPIQTIIPWATN
                     YFTESLIQQVRAEFGPAFWGFWMLGGMSGGGMGFIFAPEQKARAQTRLQELMSAGKRA
                     LQHALPFAMEPVVYDFAINERGTEAVLLEGAAALMPPAYYTLLGPALLRQERHTLTPA
                     RRAEWDQFGAACRARPEWRGVAQTLFEIMLPRGQPEAAAGQSLAALLEQHGFDRLQHE
                     QIRADLKEGRIGLAQNRLPASAVIEDAPAAEVADLSAPGPAAARWAERGRAALRNGEL
                     AVVTLAAGAGSRWTQGAGVVKALHPFCRLAGRHRTFIETHLAKSRRAARQAGCALPHL
                     ITTSYLTHAPIAEFLARQHHYGYEGPLHLSPGRAVGLRLVPTVRDLRFMWEEMPQQLL
                     DEQQQKVRDSLRQALIHWAAAAGEASDYTDNLPLQCLHPVGHWYEVPNLLRNGVLARL
                     LAERPGLQYLLLHNIDTLGADADPALLGWHIDHGACLTFEVIPRRLEDRGGGLARVNG
                     QLRLVEGLALPREEAEFALTYYNSNTCWIHLDKLLAVFRLTRADLADAPRVAAAIRSL
                     AARMPTYITLKEVKKRWGHGQEDIFPVTQFEKLWVDLTALPEVDARFVAVPRLRGQQL
                     KDPAQLDGWLRDGSAAYVERLCDYPA"
     gene            complement(7082..7927)
                     /locus_tag="PKI07_08710"
     CDS             complement(7082..7927)
                     /locus_tag="PKI07_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOC51171.1"
                     /translation="MEIKKAIITAAGPAQRTLPLQTLVDRDGVTKTALRILIEEVRAA
                     GIEEICVVTCPGDPPAYAAAAGDPGGRLRFIAQPAPRGYGHAVWCAREFAGESPFLLL
                     VGDHLYVSGGRKRAAQQLVEIAGAENCAVSAVQATHESKLPYYGAVGGRRVAGRKGQY
                     EIAEVMEKPTPTEAEQRLIVPGLRAGYYLCFFGMHVLTPAVMSLLSAEVERNAGPTLP
                     LSPALAQLAQRERYLAGELEGRRYDIGVRYGLLTAQLALALAGQDREEVLSGLVELLA
                     RREMK"
     gene            complement(7957..11256)
                     /locus_tag="PKI07_08715"
     CDS             complement(7957..11256)
                     /locus_tag="PKI07_08715"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HOC51172.1"
                     /translation="MKKNLPFPTVRRPGLTRRVLTSGAGLLLALGLMSASASAVVVGQ
                     LRCEYLKDPLGIDVAQPRLSWVLSADKSAVRGQQQSAWQVLVASSPRELAANRGDLWN
                     SGKVMSDQSIHVRYHGRALTSGQPCYWKVRVWDERGKPSAWSAPARWTMGLLQPSDWH
                     ARWIGLDESSAGEVAVKVLGDAQWIWFPEGQPEKAAPVGTRYFRRGLTLPPDRVVRRA
                     TLAWAADNSGVFHINGRKVGVASDFHSATVVEVAGDLRAGDNQLAVAVSNAGSDPNPA
                     GLVACLRVEFAQGEPLVVVTDSSWLSATAEAAGWQEKGFAETGWVAARQLGAPGMAPW
                     GEISGPESRRLAARMLRREFAVEKNIQRATAYVCGLGLSEFYLNGRKIGDHVLSPALT
                     DYTKRACYVTYDVTAQLRAGKNTAGIILGNGRFYAPRAKVPAGTVSYGYPKLLFQMQI
                     EYADGTSTEVVSDTNWRITTRGPIRANNEYDGEEYDATREMPGWNETGFDAGEWLRAQ
                     EVAAPGGALVAQMINPIRVVQTLKPIALNQPQPGVWVFDLGQNMVGWCRLKVAGPKGT
                     QVSLRHAETLQDDGTLYLDNIRGAKVTDRYTLKGKRTEVYEPRFTYHGFRFVELTGYP
                     GKPTLATLEGRVVHDDLESAGEFECSNPLLNRIYQNIVWGVSGNYRSISTDCPQRDER
                     QGWLGDRSAESKGEAYLFNTAALYAKWLQDMADAQKDSGSVPDVCPAYWPFYSDNVTW
                     PSSTVLIPEALREQFGDQAIIARHYPSAKRWMHYMSGFVTNGIIARDSYGDWCVPPED
                     PKLIHSKDPLRQTDRALLATAYFYADARLMARYAARLGLAEDAQHFTTLAAGLKEAFN
                     KRFFNASAGHYDNGSQTSCVLPLAFGLVPDNERGRVFDHLVRKITDETQNHIGTGLIG
                     GQWLMRVLTEGGRADLAYTIAAQKTYPSWGYMVEKGATTIWELWNGDTADPAMNSGNH
                     VMLVGDLGIWLYENLAGIKSDPEQPGFKHILMKPEPVGDLQFVRATHQSPYGRIVSHW
                     QRADGRFCWDITVPVNTTATVYVPAASLENLTEGGQPVHRARSVKFLRQEPGRMVFEV
                     GSGSYRFQNPI"
     gene            complement(11261..13120)
                     /locus_tag="PKI07_08720"
     CDS             complement(11261..13120)
                     /locus_tag="PKI07_08720"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOC51173.1"
                     /translation="MKTYPARKRAVVRTRRVMLAAVLAFGMNLPAEAAFLVARGGKAK
                     CVIVRQTGATVTESNAVRELAETLEKITGAKFEVRTEADGRVPKKAIVVGPGAAARAL
                     FPEVPLDKLGADELVMKVRNGRLLLAGGRPRGTLYAVNRFLQEQGGARWWTPWAASLP
                     WRDRLRVPDLNTRYQPPFEYREPFWYAGFEPRWKTRNGANGHSHEIPPALGGCVKYKG
                     FAHTFYPLVPPDQHFAAHPEWYSLIKGRRTHDHAQLCLSNPELRDFVVQRVKEWLREA
                     PDAQIISVTQNDWYGACECDRCKAIDDAEGGPSGSLLAFVNYVAERIEPEFPQVAVDT
                     FAYQYTRRPPKTIRPRPNVIVRLCSIECNFREPLDHPSNAAFLSDLQGWARICQRLYV
                     WDYTTDFRNYVLPHPNWFVLGPNVRLFQANNVKGVFEQGAYQGYGAEMGELRAWVLAQ
                     LLWNPRQDDRALIQEFLRGYYGPAAGEIIGRYLELMHHASHGFNLHCFLGKDPPHLRF
                     QPLAAAERLWQQAEAAAARGADPEWLPRVRLGHLPVRYAVLQGWNSLRRECREQGAAW
                     PWPQSRHALAEEFRQVSQGIPGQNWSKVGVLNEGGLQVEDFLKQFAQDEPDSN"
     gene            complement(13174..15153)
                     /gene="ccsA"
                     /locus_tag="PKI07_08725"
     CDS             complement(13174..15153)
                     /gene="ccsA"
                     /locus_tag="PKI07_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOC51174.1"
                     /translation="MKVLPKLFPWFLLALFGAEMVAVLLPRKDGEYHVNEFGRLPVQL
                     NGRVQPFDSAARNALLQIRGTGAVPLEEAPAWRFWRHGRKLNATEWLLEVAARPEVAD
                     TRPAFLIHHAELLGELRLQDKGVRKAGLRYYTFAELQPFLGEINEQALKAGEVKSAER
                     TAFQKQVLKLANALELYQRLKFALQPAGVPDFARMVADFQRDLGPALAAAQASQSSQD
                     FDRQALERIAEPRREFRRMARWGYIQTVPPAGAAQPRDRWQTLGVSLLESDPDGQVHP
                     AALYLATMATAYGQRNPEAFNSAVAGYRQWLAPRCERELRKGRAEFYFNRVKPFLHAL
                     IIYLGAFLLAGGALLAFALSRNWSEALRRSAFYLVLLAGTVHTFGLVFRMALEGRPPV
                     TNLYSSAIFIGWGVVLLGLILERLYRVALGSVVAAFAGFVSLIIAHNLALGGDTMEML
                     RAVLDSNFWLTAHVVTITLGYSATFAAGLLAALYLLLGVLTPLLAQPLGAEEAGAPAA
                     PGKTPPLLGQALETMVYGIVCFATLFNFLGTVLGGIWADQAWGRFWGWDPKENGALMV
                     VLWNAFILHVRSGRLLRERGFMGLVVFGNIVTSFSWFGVNLLGVGLHSYGFMESGLKW
                     LLFFMGSQLAIILVGLLPLRRWRSFRAGAGSPPAG"
     gene            complement(15326..16486)
                     /locus_tag="PKI07_08730"
     CDS             complement(15326..16486)
                     /locus_tag="PKI07_08730"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOC51175.1"
                     /translation="MMPPSFLDRVVRFLASIRLTAVCLVLALGVVFWGTLAQVEMGLY
                     RAQHEFFRSLFIYWGPARAAWRIPVFPGGYLVGGVLLVNLIAALGKRFALTPKRLGLW
                     LAHVGLILLLLGQLLSDLLSQESTLHLREGQARNYSEAERETELAVVEAAGADTDNVV
                     VIPQRLLAQEKTIAPGRLPFAVRVRKFFANSEVAEPTAAAAQPAAATQGIGRHAIVRG
                     LARATAMNTRDVPSAVVEIETPQGSLGTWLLSEFIGEPQSLVWSNRTYQLTLRPRRYY
                     TPYHVQLVRFQHEVYPGTDIPRHFSSRVRLSRADTGEQREVLIYMNNPLRYAGATYYQ
                     SGFDPDNHGTILQVVRNPSWLTPYLACALVGAGLLIQFAIHLWGFVFKRRTA"
     gene            complement(16493..17146)
                     /locus_tag="PKI07_08735"
     CDS             complement(16493..17146)
                     /locus_tag="PKI07_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51176.1"
                     /translation="MKILTRGRSLPFFRQWAFALMALAWAGCGREQVQEYQVARESPP
                     GQPPALPSGHSDMTAAAAPALDCKPPSDWQEADRGQMRVASFRVSGGEGQQAEVSVIP
                     LPEPAGTVLDNVNRWRGQVGLAGVAEAELAGLAQPVEIAGQAAKLYEAVGVHPDTGKR
                     ASILAAMMPRDGVVWFFKMSGDADLVAGQKRPFVEFLKTVSFPERAPAGQPGAAPAP"
     gene            17386..17625
                     /locus_tag="PKI07_08740"
     CDS             17386..17625
                     /locus_tag="PKI07_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51177.1"
                     /translation="MKKIILTLMVAAFVVAVQAETAKNCPLKDQCKPACCSIKAKNAE
                     QAKAECCPLGKSFCSKKAAAKQACLKSPKATDTAN"
     gene            complement(17730..19505)
                     /locus_tag="PKI07_08745"
     CDS             complement(17730..19505)
                     /locus_tag="PKI07_08745"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOC51178.1"
                     /translation="MTKSTSGKGGTANKAVLSWVDQIAKLCKPDRVYWCNGSKAERKA
                     LIAEAVASGVLLKLNPKKRPGCYYHRSNPNDVARVEQSTFMCSESQEEAGPTNNWMAP
                     KEMYERLYGYAAGSMRGRTLYVVPYLMGPPGSPMAKVGFELTDSIYVALSMGAMTRMG
                     DVALEELGNREDFNRGLHCMLDIHPERRYISHFPKDNAVISVGSNYGGNVLLGKKCLA
                     LRIGSCLGRQEGWMAEHMLILGVESPTGEKTYVAAAFPSACGKTNFAMMMPPARLKGW
                     RVWTIGDDIAWMKPGPDGRLYAINPEAGYFGVVPGTNSQSNPAAVEMISHDTIFTNVA
                     LTPDLDVWWEGKDGSPPRECLDWKGNKWTPDSREKAAHPNSRFTAPMANNPLLAPEAN
                     DPQGVPISAIIFGGRRSDTMPLVFQAFNWGHGVYVGATMGSEMTAAAVGGAGQVRRDP
                     MAMLPFCGYHMGDYLRHWINMHKAIKHPPRIFHVNWFRKDQQGAFLWPGFGENMRVLK
                     WIVDRCHARADAEESALGWVPKPDSLDLAGLSNFTPARFEQVQAIHAEEWRREIMMHE
                     ELFIKLHTRLPKELIFQRELLLARL"
BASE COUNT         3432 a         6787 c         6667 g         3381 t
ORIGIN      
        1 ggagggatgg acgacttgcg gatgatgtaa aacgaggttc caaggcggga gggagtggac
       61 gagaacgttc ggcatgtccg gaacgggcgg ccgggtgttg tcgcgcgtcc tggtttttgg
      121 gcgagatgga gccacggctg gcggggaaat ttggttcatc cgacaagttt gatcctggca
      181 tggtgcaggg cgaggagttt gaggatgaag aaggcttcgt tccgatagcc ataggcttgg
      241 cgggtgaggg ttttgatctt gttgttgacg ccttccattt tgccgctgct gagccggtgt
      301 tcccaccagc tcagaatccc gcgggcgtgg agtcggagcg ttttggccat ttgccgcagc
      361 agggcgatgc cggtttccat cgcccgggca caccactggc gcaggaagtc ctccatcgcg
      421 acgcgcgaag gcggttccca gagcaggctc agttcttctt tcaggtagtc ggcttgggag
      481 ggggggcggg aggggacggc gggggcgggg gaggtggatt gagattcggg ttgccggttg
      541 gggcgggccg agttctggca ggccggttgg cctgttccgg cggggcggcc agtgccgggt
      601 ggggtcgggt gcttaaaaac ggaatgccgg gattcccggg gagcgtttga tggctggcgc
      661 ctgagccggg cttggaaacg aggaggtttt ggcggaggac cggcggcggc gggggggcat
      721 ccgggcgcga ggccggggcg ggcaaggcgg gcgcgggcgc gtggcgggag tttttctgat
      781 tgttcgggcg gggaaaagtt tgtatatgcg cggcgaacag cggataacac gcagcttaag
      841 acgactatga aaacatcctt acttggtttt ggaatcgcgc ttggtctgta tgggtcggcg
      901 gcggcgcagc cctcactcgg cagcccgatg gcggggctgg aacaactcaa agacttcgag
      961 gccatgcgcg aatcgtcctc ggacccggac tggcataacg ggaatgcgga ttcgcggccg
     1021 atcgagccgg gggggacgct ggtgctggcc gacctgaaag ggccgggcgt gatcacgcat
     1081 ttttggaaca ccatcgccca tccggcgccg ttctatccgc ggctgctgac gctgcggatg
     1141 tattgggacg gcgaggagca cccgagcgtc gagtgcccga ttggcgattt cttcggcatc
     1201 ggccacggac tggataaatc ctttgtgtcg ctgccgatca aggtgtcgtc ggatggccgc
     1261 gggcggaact gttattggcc gatgccgttc cgcaagtcgg cgcgcattac ggtctccaat
     1321 gaaagcgacc ggcggtgcga cgcgttttac tactacctgg actggcaaaa gcacaagtcg
     1381 ctgccgcgga acacggctta tttccacgcg atgtaccggc aggagcatcc gtgcgtgatg
     1441 gggcgcaact acctgctggc cgatctccag gggcgggggc attacgtggg caccgtgcag
     1501 agcgtgcaga atatgtccgc cgggtggtac ggggaggggg atgatttctt ctttattgac
     1561 ggcgaaaccg aaccgcgcct gcggggcacc ggcacggagg attacttctg cgacggctgg
     1621 gggtttcgcc cgcatgacgg cccgttttac ggcgtgccgc tgtgggaggg ctatgcgccc
     1681 gggaaccgcg ggtcggttta tcggtttcac ctcaccgacc cggtgccgtt caagaaatcg
     1741 ctgcgggtgg aaatcgagca caaggggtcg caggagttcc cggatcgaag cgtcagcgga
     1801 ttcatcgagc gcgatgacct gatgtcctcg gtggcgttct ggtaccaggt cgagccgcat
     1861 aagccgtggc cggcgctgcc gccggggccg gagcggctgg ccgagcagac gctggccctg
     1921 gtcaccggct ggaaggcggt gggcggcgcc aaacattccg accacccgct ggcggtgcag
     1981 gacttgcccg gcatcgccaa agagggcaag caactgttct tcacgccccg aaacgatcag
     2041 ggctgggtgg aatgtccgtt ccaggtgggc aaggacatcc gggcgcatct ctggggcaag
     2101 ttcatccagt cccatgatta cggcatttac cgggtgaccc tcgatgggcg ggagctggcc
     2161 gtggtgaacc tttaccaccc ggagacccgg cgccacgtct attactgggg cccgcaggcg
     2221 ctcagcgccg gggcgcacgt tctccgcttc gagtgccagg gcaaggcgga tcattccacc
     2281 ggctactgcc tgggcctgga ttccatcgcc gcggtggtgc cggcgtacga acggccgccg
     2341 gggtttgatt taaggaaaat ccagcggtaa gccctccggg gggccacgcg gcccgcgcgg
     2401 cggaccggcc gggacgcggc ggcggcgcgc cggggcgcgg cctatggggc ttgcccgcgg
     2461 ccccgcggat gcctattact gaacccatga actcagactt gagatttacc gggaagggaa
     2521 cggccaagct ggccggggcg gtgttggccg ccctggcgtg gccgcaggcg ttgttaagcg
     2581 cggcggagcc ggcggcctgg ctgaccttcg ggctggatcg cgtgccgggg ctgggctacg
     2641 aggtgttggg caacccgctc tggcaatacc tggcctttct gatttatgtg gcgctggcgt
     2701 tctacgcctc gaagctgctg gacgcgctga ttcagcatcg cctgcagaag tgggcggccc
     2761 ggacccaaac gaagctggac gacctgctgc tggagttgct ccggggcccc atcaaggtgg
     2821 tggccttcgt ggtgtttctg cacatcgggc tgcggctgtt caactggccg gtgtgggcct
     2881 cgaagttcat ctccaacggc ctcaagatcg tcgtggcctg ctcgctgacc tacatcgcga
     2941 tcaagttcgt ggatttgctg gccgggctgt ggcacaaaca gatggcggcc catgacgacc
     3001 tggcggacca ccaactgttc cccatcattc ggaagaccct gaagatcttt gtggtcgtgg
     3061 tcgcggtgct ggtgacctcg cagaacctgg gcatgaacgt caccggcctg ttggcgtccc
     3121 tgtccattgg cgggctggcg gtggggctgg ccgcccagga cgcgctgtcc aatttttttg
     3181 gcgcggtggc catctttgcc gacaagccgt tccggattgg ggaccgcatt caactggaca
     3241 gtgtggatgg ttcggtcgag gccatcggcc tgcgcagcac gcgggtgcgg aacctggacg
     3301 ggcatctcgt caccatcccg aaccggacga tggcgaatgc caacatcgtg aacgtgagcc
     3361 ggcggcccaa tatcaagacc gtcatgaata tcgggctgac ctacgacacg cccgccgcgc
     3421 aggtggagcg cgcggtgcag atcatcgagg aaatcttccg gccgcatccc cggaccgtgg
     3481 acctgctgat cagcttcaac aagttcgagg cctcgtcgct gaacgtgctg gtcgtgcatc
     3541 gctggggctc gacggactac cccgaatacc tgcgcggttt ccagcagttg aacctggagt
     3601 tgaagcgccg cttcgatgcg gaggggatca gctttgcctt tcccacgcag acgatctatg
     3661 tgcgcccgga tcccggcgcg ccgccggcgg gcgccccggt ggcagcccgg tagcccggct
     3721 ttcccgcgcc cggctcaggc cgggtagtcg cagaggcgct cgacgtaggc cgccgagccg
     3781 tcgcgcagcc agccgtcaag ctgcgccggg tccttgagct gctggccgcg caggcgcggc
     3841 accgccacga agcgggcgtc cacctccggc agcgcggtca ggtccaccca gagcttctcg
     3901 aactgggtca cggggaagat gtcctcctgg ccgtgccccc accgtttctt gacttccttg
     3961 agggtgatgt aggtgggcat ccgggccgcc aggctgcgga tggcggcggc gacccggggc
     4021 gcgtccgcga ggtcggcccg ggtgaggcgg aacacggcga gcagcttgtc gaggtggatc
     4081 cagcaggtat tcgagttgta gtaggtcagg gcaaactccg cttcctcccg cggcagggcc
     4141 agcccctcga ccaggcggag ctggccgttg acgcgcgcca ggccgccgcc gcggtcttcg
     4201 aggcggcggg ggatgacctc gaacgtgagg caggcgccgt ggtcaatatg ccagcccagc
     4261 agggccgggt cggcatccgc ccccagcgtg tcaatgttat gcagcagcag gtattgcaag
     4321 cccggccgct ccgccaggag gcgggccagg acgccgttgc gcaggaggtt ggggacctcg
     4381 taccaatgcc cgacggggtg caggcattgc agcggcaggt tgtccgtgta gtcgctggct
     4441 tcgccggcgg ccgcggccca gtggatgagc gcctggcgca ggctgtcgcg caccttttgc
     4501 tgctgctcgt cgaggagctg ctgcggcatt tcctcccaca tgaaccgcag gtcgcggacg
     4561 gtgggaacca ggcgcaggcc caccgcccgg cccggcgaca ggtgcagcgg gccttcgtag
     4621 ccatagtgat gctgccgcgc caggaactcc gcgatgggcg catgggtcag gtagctggtg
     4681 gtgatgaggt gcgggagggc gcagccggcc tggcgcgcgg cgcggcggct cttggccagg
     4741 tgcgtctcga tgaaggtgcg atggcgcccg gccagccggc agaaggggtg cagggctttg
     4801 acgacccccg cgccctgcgt ccagcggctc ccggcgcccg ccgcgagcgt gacgaccgcc
     4861 agttcgccgt tccgcaaggc ggcccggccc cgctccgccc agcgcgcggc ggcggggccg
     4921 ggcgcggaga gatcggccac ctccgcggcg ggcgcgtcct cgatgaccgc gctggcgggc
     4981 aggcggtttt gggccagccc aatgcggcct tccttgaggt cggcgcggat ttgctcatgc
     5041 tgcaggcggt caaagccgtg ctgctccagc agcgccgcga gggattggcc ggcggcggct
     5101 tccggctggc cgcgcgggag catgatttcg aagagcgttt gcgccacgcc gcgccattcg
     5161 gggcgggcgc ggcaggcggc gccgaactgg tcccattccg cgcgccgggc cggggtgagg
     5221 gtgtggcgtt cctgccgcag cagcgccggc cccagcaggg tgtaatacgc cggcggcatc
     5281 agcgccgccg ctccttccag caggacggcc tcggtgcccc gctcgttgat ggcaaagtca
     5341 tagaccaccg gctccatggc gaacggcagc gcgtgctgca gcgcgcgttt gccggcggac
     5401 atgagctcct gcaggcgcgt ttgcgcgcgg gccttctgct ccggcgcgaa gatgaacccc
     5461 atcccgccgc ccgacatgcc gcccagcatc cagaagcccc aaaaggcggg gccgaactcc
     5521 gcgcgcacct gctggatcag ggactcggtg aagtagttgg tggcccacgg gatgatggtc
     5581 tggatgggct gctggaaatt gcgggtcgtg gcggcgccaa tccggcgcac gtcgccggcg
     5641 cgcagggcgg cgaggatttc gtcgaggatg cccagcatgc tttggcgcgc ctgccattcg
     5701 tcggcgcagc gcagcaggta tttctcggtc accatctcca ggatgggccc cacattctgg
     5761 gccatgccgc cgtggacgag cacgaggctt tcctgcagcc ggcggcgcgt ttccagcgcg
     5821 gcgtcggcgg ccgccaggat gcggtgccgc ggcatcagcc ggccccggct gacgccgaac
     5881 tccgggtcgc cctcgccggc cggcacgcct tcaatcagct tcatccccgg ccagacgccg
     5941 cccgagtcct gccagccgcc gccggagccg ccgagccatt ctcccagcag cgcccgcgcc
     6001 agcaccagcc ggcgttcgtg ctcctgcagc ggccccgtga gcgaggcggc ctgcccggtg
     6061 gcccgcatgc agacgctgac cagcgcggcc agcagcgtcg tcgagaccgc caggcgcgag
     6121 cctttgggga tgttattcac gcggctgacg atctccaggc cgcggccccg cccggccagg
     6181 cgcgccagca actccgccag gctctggccc gagccttcaa tgcccggcgg caccaggccc
     6241 gcggcgatca ccgccgcctt gagcaggccg aggtaatcct tcgcgaagtc gaacacttcc
     6301 gccaggttcg tgatgtccgc cgccgcgccc aggtcaaggc tggccagccg cagcagcggc
     6361 tcttcgatca cgcgcaggct ggcctccacc ggggggcgcg gcgcgggatc gcgcccgtga
     6421 acgcccaggt caatcgagac gttgagcacc ttggcgccct cggggtagtc catgccgagg
     6481 aagaaaatat cgctccaccc gcagtgcgtg aggtccatgc gcaccggggt gcattcgcgc
     6541 aggatggggt aggtgccgtc gcccgggtgg ggctggcaca gttcgggccg caggcgcagg
     6601 gggtggtcgg cgggatggcc catgcggaac atccactggt tgccgcgcac cgaacgcacg
     6661 ctccggcgga cctggtccgc cagcgtctgg aacccgagcc ggtggtacgc cgccgccagc
     6721 gcgctggaga tggcatcgct cgggccttcc gcctgctgca cggccagaaa ctggtccagg
     6781 gcctcctcga agcggcgctg gagcaaatgc tcgtagccgg cgaaggggat gagcgagcgg
     6841 cggccggcgg gggcggcggc ggacgccagg cgttccggca aatggaaccg gtggatcgcg
     6901 taaaggaaga acagcgcgcg cacgcgctcg tagaggtttt cgctgcgccg gcggaagtcg
     6961 tccagccgcg cgcattggtc caggaggtcg ccggcggaca ggccggcgca ggcggcggcg
     7021 agcgcctggt tgcgccgggc ggggtcggcc gcggtgatga gctggaccag gtcgggcatg
     7081 gtcacttcat ttcccgccgc gccagcaact cgaccagccc gctcaacact tcctcccggt
     7141 cttgcccggc cagcgccagg gccagttgcg ccgtcagcag gccgtagcgg acgccgatat
     7201 cgtagcggcg cccttcgagt tcgccggcca ggtagcgctc gcgctgggcg agctgggcca
     7261 gggccgggga aaggggcagg gtcgggccgg cgtttcgctc cacttccgcg ctgagcaggc
     7321 tcatcaccgc cggcgtgagc acatgcatgc cgaagaaaca caggtagtag cccgcccgca
     7381 gtccggggac aatcagccgc tgctcggcct cggtgggcgt gggcttttcc atcacttccg
     7441 cgatctcgta ctgtcccttg cgcccggcga cccggcggcc gccgaccgcc ccgtaatacg
     7501 gcagcttgct ttcgtgcgtc gcctgcaccg ccgagaccgc gcagttctcg gcgcccgcga
     7561 tttccaccag ttgctgggcc gcgcgcttcc ggccgccgct gacatagagg tggtcgccca
     7621 ccagcagcag gaagggcgac tcgccggcga actcgcgcgc gcaccacacc gcgtggccat
     7681 agccccgggg cgcgggctgc gcgatgaagc gcaggcgccc gcccgggtcg ccggcggccg
     7741 cggcataagc cggcgggtcg ccggggcaag tcacgacgca gatttcctcg atgcccgccg
     7801 cccgcacttc ttcgatcagg atgcgcagcg cggtcttggt gacgccgtcg cggtccacca
     7861 gggtttgcag gggaagggtc cgctgcgccg ggccggcggc ggtgatgatg gcttttttga
     7921 tttccacagt cagaagcgga gccgggggcc ccggggttag atgggattct ggaagcggta
     7981 gctgcccgag cccacctcga agaccatgcg gcccggttcc tggcgcagga acttcacgct
     8041 gcgggcccgg tggacgggct ggccgccttc ggtcaggttt tcgaggctcg ccgccggcac
     8101 gtacaccgtg gcggtggtgt tgaccggcac ggtgatgtcc cagcagaacc gcccatccgc
     8161 gcgctgccag tggctgacga tccgcccgta gggggactgg tgcgtggccc ggacgaattg
     8221 caggtctccg accggttcgg gtttcatgag gatatgcttg aagccgggct gctccgggtc
     8281 ggacttgatg cccgcgaggt tttcgtacag ccagatgccc aggtcgccca ccagcatgac
     8341 gtggttgccc gagttcatcg ccggatcggc ggtatcgcca ttccacagct cccagatcgt
     8401 ggtcgcgccc ttttcgacca tgtagcccca actggggtag gttttctgcg cggcgatggt
     8461 gtacgccagg tccgcccggc cgccctccgt cagcacgcgc atcagccact ggccgccgat
     8521 cagcccggtg ccgatgtggt tctgcgtttc gtcggtaatc ttccgcacca ggtggtcgaa
     8581 cacccgcccg cgttcgttgt ccggcaccag gccaaacgcc agcggcagca cgcaggacgt
     8641 ttgcgagccg ttgtcgtagt ggccggcgct ggcattaaag aaccgcttgt tgaaggcttc
     8701 cttcagcccc gccgccaggg tggtgaaatg ctgcgcatct tcagccaggc ccagccgggc
     8761 cgcgtagcgg gccatgagcc gcgcgtccgc gtagaaataa gccgtggcca gcagcgcccg
     8821 gtcggtctgg cgcaggggat ccttggaatg gatcagtttg gggtcctccg gcggcacgca
     8881 ccagtcgcca taggaatccc gcgcgataat gccgttggtg acgaagccgc tcatgtagtg
     8941 catccagcgt ttcgcgctcg ggtagtgccg cgcgataatc gcctgatcgc caaactgctc
     9001 gcgcagcgcc tcgggaatca gcacggtgct gctcggccag gtgacgttgt ccgagtagaa
     9061 cggccagtag gccgggcaga catccggcac gctgccgctg tccttctgcg cgtcggccat
     9121 gtcctgcagc cacttggcat acagcgcggc cgtgttgaac aggtaggcct cgcctttcga
     9181 ttcggcggac cggtcgccca gccagccctg gcgctcatcg cgctgcgggc aatcggtcga
     9241 gatgctgcgg tagttgccgc tcacccccca gacgatgttc tggtagatgc ggttcagcag
     9301 cgggttggag cattcaaatt cgccggcgct ctccaaatca tcgtgcacca cccgcccttc
     9361 cagcgtggcc agcgtcggtt tgccgggata cccggtcagc tcgacaaagc ggaatccgtg
     9421 gtaggtgaat cgcggttcat acacttcggt gcgcttgcct ttcagcgtgt agcggtcggt
     9481 caccttcgcg ccgcggatgt tgtccagata tagcgttccg tcgtcctgca gcgtctcggc
     9541 atggcgcagg gagacctggg ttcccttcgg gccggcgacc ttgagccggc accagccgac
     9601 catgttctgc cccaggtcaa acacccaaac gcccggttgg ggctggttca gcgcgatcgg
     9661 tttcagggtt tgcaccaccc ggatcgggtt aatcatctgc gccacgagcg cgccgccggg
     9721 ggccgccacc tcctgcgccc gcagccattc tccggcgtca aagccggtct cgttccagcc
     9781 gggcatttcc cgggtcgcat catattcctc gccgtcgtat tcgttattgg cccgaatcgg
     9841 cccccgcgtg gtgatccgcc agttcgtgtc gctcaccact tccgtggaag ttccatccgc
     9901 gtattcgatc tgcatttgga acaagagctt gggatacccg tagctcaccg tgcccgccgg
     9961 caccttcgcg cgcggcgcgt agaaccggcc gttgcccagg atgatgccgg cggtgttttt
    10021 ccccgcccgg agctgcgccg tcacgtcgta ggtcacgtag caggcgcgct tggtatagtc
    10081 cgtcaacgcc ggcgacagga cgtggtcgcc gattttgcgg ccattgaggt aaaactcgct
    10141 caggcccagc ccgcaaacgt aggccgttgc ccgctggatg ttcttttcga cggcaaactc
    10201 ccggcgcagc atccgcgccg ccagccggcg gctttccggt ccgctgattt ccccccacgg
    10261 cgccatgccc ggcgcgccca gttggcgggc cgccacccag ccggtttcgg caaagccctt
    10321 ttcctgccag ccggcggcct cggcggtggc gctcagccaa ctggaatcgg tcaccaccac
    10381 caggggctcg ccctgggcaa actccacccg caggcacgcc accagcccgg ccggattcgg
    10441 atccgagccg gcattggaca ccgccacggc cagttggttg tcgcccgccc gcaagtcccc
    10501 ggccacttcg accacggtgg cggagtggaa atcgctcgcc accccgacct tccggccatt
    10561 gatgtggaac acgccgctgt tatccgccgc ccaggccagc gtggcccgcc tcaccacgcg
    10621 gtccggcggc agggtgagcc cgcgccggaa gtagcgggtg ccgacgggcg cggctttttc
    10681 cggctggcct tcggggaacc aaatccactg cgcgtcgccc agcactttca ccgccacctc
    10741 gcccgcggac gactcgtcca gcccgatcca ccgggcgtgc caatccgagg gctggagcag
    10801 gcccatcgtc cagcgcgccg gcgcgctcca ggccgagggt ttgccgcgtt cgtcccagac
    10861 ccgcaccttc caatagcagg gctggcccga ggtcagcgcg cggccatggt agcggacgtg
    10921 aatggattga tccgacatca ccttgccgct gttccaaagg tcgccccggt tggccgccag
    10981 ctcccgcggg ctgctggcca ccaggacctg ccaggcgctt tgctgctggc cgcggacggc
    11041 ggacttgtcc gcgctcaaca cccagctcag gcggggctgg gcgacatcaa ttcccagcgg
    11101 gtccttcaag tattcgcatc gcaactggcc gacgacgacc gccgaagcac tggcggacat
    11161 cagccccaag gccaggagca gcccggcacc ggaggttaag acgcgccggg tcaggcccgg
    11221 gcgccgaacg gttgggaatg gcaggttttt cttcatagca tcaattagaa tcgggttcgt
    11281 cctgggcaaa ttgcttcagg aaatcctcca cttgcaggcc gccctcgttg agcacgccca
    11341 ccttggacca attctggccc gggattccct gggatacctg gcggaactcc tccgccaggg
    11401 catggcgcga ctggggccac ggccaggccg cgccttgctc ccggcactcc cgccgcagcg
    11461 agttccaccc ctgcagaacg gcgtagcgca ccggcaggtg ccccaaccgg acgcgcggca
    11521 gccattccgg gtccgccccc cgggcggccg cggcttccgc ctgctgccaa agccgttccg
    11581 ccgccgccag cggctggaac cggaggtgcg gcgggtcttt gcccaaaaaa caatgcagat
    11641 taaagccgtg ggacgcgtgg tgcatcaatt ccagatagcg cccgatgatt tcgcccgccg
    11701 ccgggccgta gtagccccgc agaaactcct gaatcagcgc ccggtcatcc tgccgcgggt
    11761 tccagagcag ttgggccagc acccacgccc ggagctcgcc catctccgcg ccgtagccct
    11821 ggtaggcgcc ctgctcaaac acgcccttta cgttattcgc ctggaacagg cgcacgttcg
    11881 gccccaggac aaaccagttc gggtgcggca gcacgtagtt gcggaaatcc gtcgtgtagt
    11941 cccacacata cagccgctgg caaatccggg cccagccttg caggtccgac aaaaaggccg
    12001 cgttggaagg gtggtccagg ggctcacgga aattgcattc gatcgagcag agccgcacaa
    12061 tcacattcgg ccgcggccgg atcgtcttcg gcggccggcg cgtgtattgg taggcaaacg
    12121 tgtccaccgc cacctgcgga aactccggct cgatccgctc cgcgacgtag ttgacaaagg
    12181 ccagcaggct tcccgagggg ccgccttcgg cgtcgtcaat cgccttgcac cgatcgcatt
    12241 cgcaggcgcc ataccagtca ttttgcgtca ccgaaatgat ctgcgcgtcc ggcgcctcgc
    12301 gcagccactc cttgacgcgc tgaacgacga agtcgcgcag ttccgggttg gacaggcaaa
    12361 gctgggcatg gtcatgcgtg cgccggcctt tgatcaggct gtaccattcg ggatgcgcgg
    12421 cgaaatgctg atccggcggc accagcgggt aaaaggtatg ggcgaagccc ttgtatttga
    12481 cgcagccccc aagcgccggc gggatttcgt gcgaatggcc gttcgccccg ttgcgcgtct
    12541 tccagcgcgg ctcgaagccc gcataccaaa acggctcgcg gtactcgaac ggcggctggt
    12601 agcgcgtgtt caaatccggc acgcgcaggc ggtcgcgcca gggcagactc gccgcccagg
    12661 gcgtccacca gcgcgccccg ccctgctctt gcagaaagcg gttcaccgcg tagagcgtgc
    12721 cgcgtggccg tccgcccgcc agcagcagcc ggccgttcct gaccttcatc accagttcat
    12781 cggcgcccag tttgtccagg ggcacctccg ggaacagggc ccgggccgcc gcgccggggc
    12841 ccaccacgat ggcctttttc ggcacccgcc cgtccgcctc ggtccgcacc tcaaacttcg
    12901 ccccggttat cttctccagc gtctcggcca gttcgcgcac cgcgttcgac tccgtcaccg
    12961 tcgccccggt ctgccgcaca atgacgcatt tcgccttgcc gccgcgggcg acgaggaacg
    13021 ccgcctcggc cggcaggttc attccgaaag ccagcaccgc ggccagcatt acgcgccggg
    13081 ttcgcactac cgctcttttt cgcgcagggt aagttttcac gcggcataga ctcgcattcc
    13141 tgcgcccgcg cgcaacccta aacacacgcg cgtttaaccg gcgggcggag accccgcccc
    13201 ggcgcgaaag ctgcgccagc gcctcagcgg cagcagcccg acgaggataa tggccaactg
    13261 gctgcccata aagaacagga gccacttcaa cccggattcc atgaagccgt aggaatgcag
    13321 gccgacgccg agcaggttca cgccaaacca ggaaaagctg gtcacgatat tgccaaagac
    13381 caccagcccc ataaagccgc gctcgcgcaa cagccgcccg gagcggacgt ggaggatgaa
    13441 cgcgttccaa agcacgacca tcagggcgcc gttctccttg ggatcccagc cccagaaccg
    13501 gccccaggcc tggtcggccc aaataccccc cagcaccgtg ccgaggaagt tgaacagcgt
    13561 ggcgaagcag acaatgccat agaccatcgt ctccagcgcc tgccccagaa gggggggcgt
    13621 cttgcccggg gcggcgggcg ctcccgcttc ctccgcgccc aggggttgcg ccagcaaggg
    13681 cgtcaagacc ccgagaagaa gataaagggc cgccagcagc ccggcggcga aggtcgccga
    13741 atagcccagc gtgatggtga cgacatgggc ggtaagccag aagttggagt ccagcaccgc
    13801 gcggagcatc tccatcgtgt cgccgcccag cgcgagattg tgggcgatga tcaacgaaac
    13861 gaagccggca aacgcggcga cgacgctgcc cagggcgacc cggtaaaggc gttcgagaat
    13921 cagcccgagc agcacgacgc cccagccgat aaagatggca gacgaataaa gattggtcac
    13981 cggcgggcgg ccttccagcg ccatgcggaa gaccaggcca aaggtatgca ccgtgccggc
    14041 cagcaggacc aggtaaaacg cggagcggcg cagcgcctcc gaccagttgc gcgacagggc
    14101 aaaggccagc aacgcgccgc ccgccagcag aaacgccccc aggtaaatga tcaaggcgtg
    14161 aaggaagggt ttgacgcggt tgaaatagaa ctcggcccgg cctttccgca gctcacgctc
    14221 gcagcggggc gccagccatt gccggtagcc ggcgactgcg ctattaaagg cctccgggtt
    14281 ccgctggcca tacgcggtgg ccatggtagc caaatagagg gcggccggat gcacctgccc
    14341 gtccgggtcg gactccagca gactgacccc cagggtttgc cagcggtcgc gcggctgggc
    14401 ggcgcccgcg ggcggcacgg tctgaatata gccccatcgg gccatgcgcc ggaactcccg
    14461 ccgcggctcc gcgatccgct cgagcgcctg ccggtcaaag tcctggctgc tctggctggc
    14521 ctgcgccgcc gcgagggccg ggccaaggtc ccgctgaaaa tcggcgacca tccgggcgaa
    14581 gtccgggacg ccggcgggct ggagcgcgaa cttgagccgc tggtaaagtt cgagggcgtt
    14641 ggccagcttg agcacctgct tctgaaacgc cgtccgctcc gcggatttca cctccccggc
    14701 cttgagggcc tgctcgttga tttcaccgag gaacggctgg agctcggcaa aggtataata
    14761 gcgcaggcca gccttccgga cgcccttgtc ctgaagccgg agttccccca gcagctcggc
    14821 atggtgaatg agaaaggcgg gccgggtatc ggcgacttcg ggccgcgcgg ccacttcgag
    14881 cagccactcc gtcgcgttca gcttccggcc atgacgccag aaccgccagg cgggcgcctc
    14941 ctccagcggc accgcgcccg tgccgcggat ttgcagcagc gcgttgcgcg ccgccgaatc
    15001 aaacggctga acgcggccgt tgagctgcac cggcaggcgg ccaaattcgt taacatgata
    15061 ttcgccgtcc ttccgcggca gcaggaccgc caccatctcc gccccgaaca gggccagcag
    15121 gaaccaggga aacagtttgg gcaaaacttt cataagctcc gcaggcgggc cggcgccggg
    15181 ggcccggcgc aaaacgcgat ccgcccggcg caaaggttcc ccgggcgggc ggccgctccg
    15241 gcgcggccag ctcgagggga agcgccgggc aaatccggac gcgcgcggcc gcttgccttt
    15301 cccgccgggt taatgcgccg ccagctcacg ccgtcctccg tttgaaaaca aagccccata
    15361 gatggatcgc aaactggatt agcaggcccg cccccaccag cgcgcaagcc aggtaaggcg
    15421 tcagccaact gggattgcgg actacctgca ggatggtgcc gtggttgtcg ggatcaaatc
    15481 ccgactggta gtaggttgcg cccgcataac gcagcgggtt gttcatataa atcaaaacct
    15541 cccgttgctc gccggtgtcc gcgcgagaca gccgcacgcg gctggaaaaa tgcctgggaa
    15601 tatccgtgcc cggatatacc tcgtgctgga accgcaccag ttggacgtgg tagggcgtat
    15661 aataccgccg cggccgcagg gtgagctggt aggtgcggtt gctccagaca aggctctgcg
    15721 gctcgccaat gaattcggag agcagccacg tgcccaggga accctggggg gtctcaatct
    15781 ccaccacggc gctgggcaca tcgcgcgtat tcatcgccgt cgcccgggcc agcccccgaa
    15841 cgatcgcgtg ccggccaatg ccctgcgtcg ccgccgcggg ctgcgccgcc gccgccgttg
    15901 gctccgccac ctcggaattg gcgaaaaact tcctcacccg cacggcaaac ggcaggcggc
    15961 ccggcgcaat cgtcttctcc tgcgccagca gccgctgcgg gatgaccacc acgttgtccg
    16021 tatcggcccc agccgcctcc accacagcca gctccgtctc gcgctcggcc tcggaatagt
    16081 tccgggcctg gccttcgcgc aagtgaagcg tgctctcctg cgaaagcaga tcgctcagaa
    16141 gctggccgag cagcagcagg atcaatccca catgcgccag ccacaagccc agccgtttgg
    16201 gcgtcagggc aaagcgcttg ccgagcgccg cgatgaggtt aaccagcagc acgccgccca
    16261 ccaggtagcc gccgggaaac acgggaatcc gccaggcggc cctggccggc ccccagtaga
    16321 tgaacaagct gcggaaaaac tcatgctggg cacggtaaag gcccatctcc acctgcgcca
    16381 gcgtgcccca gaaaaccacc cccagcgcca gcaccaggca gaccgccgtc agccgaatcg
    16441 aggccaggaa gcgaaccaca cgatccagaa aggagggcgg catcatgaaa cctcaaggcg
    16501 ccggcgcggc gcccggctgg ccagccgggg ctctttccgg gaaactgacg gttttgagaa
    16561 actcaacgaa cggccgcttc tggccggcca ccagatccgc atcgccgctc atcttaaaga
    16621 accagacgac gccatcccgg ggcatcatcg cggccaggat gcttgcccgc ttgcccgtgt
    16681 cgggatggac gccgaccgcc tcgtagagct ttgccgcctg gccggcaatc tcaaccggct
    16741 gcgccagccc ggccagttcg gcctccgcca cgcccgccaa acccacctgg ccgcgccagc
    16801 ggttcacatt gtccaaaacc gtcccggccg gttccggcag cgggattaca ctgacctccg
    16861 cctgctggcc ctccccgccc gacacccgaa aagaagcaac ccgcatttgc ccccggtccg
    16921 cttcctgcca atccgacggc ggtttgcaat ccagcgcggg cgcggccgcc gcggtcatat
    16981 cggaatgtcc cgagggcaat gccggcggtt gccccggcgg cgattcccgg gccacctgat
    17041 actcctgcac ctgctcccgc ccgcaccccg cccaagccag cgccatcagg gcaaaggccc
    17101 attgtctgaa aaacgggagg cttcgccccc gggtcaatat tttcaccgcg cgtacctaca
    17161 accattttcg ccccagcgca agccgccgct ccgccaattt tcccgcgggg acgcattctg
    17221 ccatggattc cggttctccg ggcctgaagt tcacgttttc gaccgggacc cttctgcgcg
    17281 ccatccccgt ggttggaacc ctttttgcgc gcccaaaccc tttccgggcg gctaggcaaa
    17341 aaataatact tgaaataagc agatcaaacg gttatatacc acgatatgaa aaagatcatc
    17401 ctcacactga tggtggcggc gttcgtggtt gcggtgcaag ccgagacggc caagaactgt
    17461 ccgcttaagg atcaatgcaa accggcttgt tgctccatca aagctaaaaa cgcggagcag
    17521 gccaaagcgg aatgctgccc cttgggcaag tccttctgca gcaagaaagc cgcggccaag
    17581 caagcgtgcc tgaaaagccc caaggcgaca gacacggcta attaagcctg gagctttctc
    17641 ctcaaaatcc ccgcgtgccg caaggcgcgc ggggattctt tatttccacc cccggggcgg
    17701 gcgaatgtcg ggcaaggcgc ccgccgcggc tacaagcggg ccaggagcag ctcgcgctgg
    17761 aagatcaatt ccttgggcag gcgggtatgc agtttgatga aaagctcctc gtgcatcatg
    17821 atttcacgcc gccattcctc ggcgtgaatg gcctgcacct gctcgaagcg cgcgggcgtg
    17881 aaattggaca gaccggccag gtcgaggctg tccggcttgg gcacccagcc cagcgcgctt
    17941 tcctccgcat cggcccgggc gtggcagcgg tccacgatcc atttaagcac gcgcatgttc
    18001 tccccaaagc cgggccagag gaacgccccc tgctggtctt tgcggaacca gttgacatgg
    18061 aaaatccgcg gcgggtgctt gatcgccttg tgcatgttga tccagtgccg gagatagtcg
    18121 cccatgtggt agccgcagaa cggcagcatg gccatcgggt cgcgccgcac ctggccggcc
    18181 ccgcccaccg cggcagcggt catctccgac cccatggtgg cgcccacata cacgccgtgg
    18241 ccccagttga acgcctggaa aaccagcggc atggtatcgc tccgccgccc gccgaagata
    18301 atcgcgctga tcggaacccc ctgggggtca ttcgcttccg gggccagcag cgggttattg
    18361 gccatcgggg cggtaaagcg gctgttggga tgcgcggcct tctcccgcga atccggcgtc
    18421 cacttgttcc ctttccagtc gaggcactcc cggggcgggg aaccatcctt gccctcccac
    18481 cagacatcca ggtcgggcgt aagcgccaca ttcgtgaaaa tggtatcgtg ggaaatcatt
    18541 tccacggcgg ccgggttgga ctgcgaattg gtgccgggca caaccccgaa gtagcccgct
    18601 tccggattga tggcatacag gcgcccatcc ggcccgggct tcatccaggc aatgtcatcg
    18661 ccaatggtcc acacccgcca tcccttgagc cgggcgggcg gcatcatcat ggcgaaattg
    18721 gtcttgccgc aggcgctggg gaaagccgcc gcgacgtagg tcttctcacc cgtgggcgat
    18781 tccaccccca aaatcagcat gtgctcggcc atccacccct cctggcgccc gagacaggag
    18841 ccgatgcgca gcgccaggca cttcttcccc agcaacacgt tgccgccata attcgagccc
    18901 accgatatga ccgcgttatc cttgggaaag tgcgagatat agcgccgctc cgggtggata
    18961 tccagcatgc agtgcagccc ccggttgaaa tcctcgcggt tgcccaactc ctccagcgcg
    19021 acgtcgccca tccgcgtcat ggcccccatg cttaacgcca cataaatcga atcggtcagt
    19081 tcaaacccca ccttcgccat cggcgaaccg ggcggcccca tgaggtaggg aacgacatac
    19141 agcgtgcgcc cgcgcatcga gcccgccgca tacccataca gccgctcata catttctttc
    19201 ggcgccatcc agttattggt cgggccggcc tcctcctggc tttccgagca cataaacgtg
    19261 gattgctcca cccgcgccac atcgttggga ttcgaacgat ggtaatagca gcccggccgc
    19321 ttcttcgggt tgagtttaag caaaaccccg ctggcgaccg cttccgcgat cagcgccttg
    19381 cgctccgctt tggagccgtt gcaccaataa acccggtccg gcttgcaaag tttggcgatc
    19441 tggtcaaccc aggacagcac cgctttattg gccgtgccgc cctttccgct cgttgatttc
    19501 gtcataaaca gtcttttcgt cgcgcaggct ggtacccgat tcgcgacccg attgcaagaa
    19561 acgttcttca atgagcggcg attgttctaa acgtgaatgt gcgtatttgc cgaataccgg
    19621 ccgcccaaat gcccggcccg gccgggtttt cccaaacaga atcaccaggc gacaagcctg
    19681 aaatgtttgg cggaagggtg cgtgaacgtc cggggctttc gcagggggag tccgcctgcc
    19741 aaagcgcggc cctgacaatg gcccgccaac agcatcgcgg attggggccg ggccgaagtg
    19801 cttggcggaa ctggaacccc caaacttccc tgggccagcc ccctcggggc ggaccgccgc
    19861 tatggctcca ttcagttgct cttatacgga acgcatcagt tggggagccc aaccgcccgc
    19921 ccgcgttcca tcagagcctg gacagccctg accgcccaga acatagtctc ggcgagccca
    19981 ttattttgcg aaatccgcgg catcgctgct gaaccccaaa cgtcttttac gtgtaaaatc
    20041 aatgacttac acacataggc agggatgaaa tgcaggaaaa atccaactcg ctccggctga
    20101 accgcgcctt ggacgcccca tagacgcacc ttgcatttcc cctgccccca ggccgtgtac
    20161 aaggggtgta caagggatgc ccaccgggag ttcatagggt tttagctggt acacccgatg
    20221 ggcatccctt gtacacccct tgtacacggc ctgggggcag gggaaaa
//
LOCUS       DAOPQG010000066        20201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3286_length_20201_cov_15.837784, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000066 DAOPQG010000000
VERSION     DAOPQG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 20201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20201
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3286_length_20201_cov_15.837784"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKI07_08750"
     CDS             complement(<1..413)
                     /locus_tag="PKI07_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51179.1"
                     /translation="MKPTTYCLIGSPCKAAARAAAWMLPLLGLLLAGANLAAATPSFV
                     AAKPLWPKGRETEKNITVGFRASFNAPARDKVLLRATGSSIYRVFLNGQFLAHGPARG
                     PHGFFRVDEWDLTDKLKPGANVVAFEVAGYNANSY"
     gene            1657..3591
                     /locus_tag="PKI07_08755"
     CDS             1657..3591
                     /locus_tag="PKI07_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51180.1"
                     /translation="MTPATTASTERKPAAPRHTSRNPWLWVPSLYFSEGIPYVIVMTM
                     SVVMYKRLGISNTDIALYTSWLYLPWVIKPLWSPFVDITRTKRFWIIAMQYLVSLGLA
                     LVAFSIQGPAFFKWSLFLFWIMAFASSTHDIAADGFYMLGLTKHEQAWWVGLRSTFYR
                     TAMIVGSGLLVVLAGVLESKNGLPPVEIPVQASTQAPVVEAWDPAAVRFTAPDGELRV
                     LAQPNPLPIALTERSAEEARAVIAQARAWNRQHGFIAEENPAPAKAESDAPSWWTANV
                     SGPLGGLLTNYFPRPPRSVSSQAPVAGNVGVLYLSLSQEPPAGQEIAVNFGRQPGGLE
                     YIGLGKSDKGFSLKEGERFVFNAANWNQPAMAVIQLDPKLKADTAVTFATSSGNIPLA
                     WSITLFFVAALFLAFSFWHTVMLPRPASDGPVVTHHSLLGEFFGTLGSFFKKPGVVLS
                     MAFILLYRFDEAQLVKVISPFLLDSREAGGLGLTTSQVGVVYGTFGILALTFGGLLGG
                     FMAAKHGLRRMLPIMVASMYLPKFAFVFLSWAQPTNFLITCAAVAVEQFGYGFGFTAF
                     MLYMLFFADGPHKTAHFAICTGFMALGMMIPGMWSGWVADLVGYKHFFVWVICSAIPG
                     FTLAMLLYRRIDPQFGKKTS"
     gene            3627..3848
                     /locus_tag="PKI07_08760"
     CDS             3627..3848
                     /locus_tag="PKI07_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51181.1"
                     /translation="MRAVFPSRVIGFFIFAGRATYTTEKNFFTAVLSAESFRRWQLKL
                     SVVFPNTCSQRAISVFCGKVAAKEKKESG"
     gene            4025..4681
                     /locus_tag="PKI07_08765"
     CDS             4025..4681
                     /locus_tag="PKI07_08765"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HOC51182.1"
                     /translation="MKRLMKSVVLGLVGASLLVGTARAEIYITNSVGPFTDTLAPATT
                     EWNHVFQVQAFDAHLGSLVAVYVEVTENVDVSGFVQNTSAQKQSFFFRSGSVLTVTLP
                     GALGFLQPNPLALTQTYTDLAPGATAPYGPASASESVDNMYFAPLSGWTSYTGQMVKF
                     PAYTLTDERITGGGGNIVAAMNTIAGATVSVTYYYIPEPGSLALLALGGAALLLRRRK
                     "
     gene            complement(5082..6095)
                     /locus_tag="PKI07_08770"
     CDS             complement(5082..6095)
                     /locus_tag="PKI07_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOC51183.1"
                     /translation="MRTLRWFFLLGILSFCAVPKPAVAQTGVGMHLYAGITITGVVNC
                     VYAIQTTTDLSNPNSWQDLAIITLPSSPYLYFDTQNPACGNRFYRAVLQLVMTNMIYI
                     SPGTFTMGSPTNEVGRYADEDQHTVVLTRGFYIGKYPVRQAEYLAIMGNNPSYFTPAN
                     GYPECTLNCPVETVSWHDATNYCGKFTRQELAAGRLAPGWQYRLPTEAEWEYACRAGT
                     TTRFSYGDDPDYSNLAAYAWYKSNSSVVTHTVGYLKANPWGLYDMHGNVCEWCQDWYG
                     EYPTGTVTDPQGPTSGVAHRVIRGGNYFSYPGSYCRSAQRANLAATNTRKDTGFRIVL
                     APL"
     gene            6696..9368
                     /locus_tag="PKI07_08775"
     CDS             6696..9368
                     /locus_tag="PKI07_08775"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51184.1"
                     /translation="MTTGRPWLLAGGMLAVYLATLSCGVTAGNLRSVVELSGWNWQPE
                     VAAPVTFLLTYPLRWLPARGIAPAANLCSALCAALTLALLARSVALLPGQRRISLLSP
                     RMAWLPPALAVFACGLQLTFWENAIQAAGEILDLLLFAAAAWCVLEFRVTRQPSWLTR
                     FAVGYGLALANNWAMAGFLPLFLVAFLWTRPLHLLDFRFAARALRLGWKAVAPELTAD
                     FRLCLRLALCGLAGLSLLLLLPLLAHFSTTRHFDFWPALLSELGAYKHMLFRFPKGVI
                     LLLSLTSILPALFMGIGRWSLRFQSDSRSPKSLAVRGVFYLIHAAWLLACIWVALDCP
                     VSPRHRGLGFAFLPLYYLGALSIGYFSGYFLLVFGARNPNGLPRRSLEKLAAAATAGI
                     GLLLLIVPSLLLYRNLPQIRAIHRDPWLGYFTLVEKSLPAQGSVILSDSSLPLLFFQA
                     ALVRDSKPFPYLPLDATALDKPAYQAFLGRAYPRFGLAGTQSNLFAECASPVGRVRLL
                     EALAKRHGLYYLHPSLGYYFEAFYPQAQGLVYQLKPYGSNVAIAPLPSPELIARNRQF
                     WQEAETNQFAALARALQQGSPPAIPNWIQRLLARIHLQNEPNRSAQIVGARYARTLNY
                     WGAELQEGRAYSEAAQYFRQARELNPDNVAARVNQDFNAELQAGRPPVLAPATAIEEE
                     AGYRRKWEQVILEDGPFAEPNYCYQVGSALARNGLYHQALQQFRRVQTLAPDLTDARF
                     WMARLFIHTGHYSNALAAVNQILQAHPDDDNALFLKAVALLQSKAYEESLVPFTYLIN
                     HYPSNYAAQLNRAIAYLQLGRLDAARRDYEQVAQALPRSYQAWFGLAEIAYRRKDFPA
                     AITNYQRYLTNAPPDTQEAGQVRARLEELQRNSP"
     gene            complement(9398..10087)
                     /locus_tag="PKI07_08780"
     CDS             complement(9398..10087)
                     /locus_tag="PKI07_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOC51185.1"
                     /translation="MQAQPWHLLQSGPADPSTNMALDEALLRAAPRLGQPVLRFYGWN
                     EPAASFGYFQKYADVERLTHLRPLVRRPTGGGLVPHDADWTYSLAIPPSHFWYALCAR
                     ESYRRIHEWIQSAFQLIGLATELAPLPNPTAPGQCFQGHEAADLLWQGRKIAGAAQRR
                     TRSGLLIQGSVQPRDLSLARADWEQAMRAAGAAGQGLAWAEFKPDAQLLEETRALTAQ
                     KYSRADYHQKR"
     gene            complement(10180..10842)
                     /gene="nth"
                     /locus_tag="PKI07_08785"
     CDS             complement(10180..10842)
                     /gene="nth"
                     /locus_tag="PKI07_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOC51186.1"
                     /translation="MPRKIKDAPGVPLRKIMASLAKAYPNAHCELNYSNPLELLVATI
                     LSARCTDQRVNLVTAKLFKKYRSAADYARVPLPELEQDIRTTGFYRNKARSLKGCGQA
                     LVERHDGQVPNTMEALTQLDGVGRKTANVVLGNAFHLNYGVVVDTHVARLAQRLGLTQ
                     EKDPEKIEQALMALVPQAQWTQFSHWLIWHGRRRCTARKPDCRGCELRALCPRIGVTT
                     PI"
     gene            complement(10921..13143)
                     /locus_tag="PKI07_08790"
     CDS             complement(10921..13143)
                     /locus_tag="PKI07_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51187.1"
                     /translation="MNTNHSWKFFRAGGFDQVKLETGADLMALDQLDQKLWVALACPT
                     TGLEFDQATLALIDTDKDGRVRAPELIAAVKWAGGLLKNPDDLIKGSPTLRLAAINEA
                     APEGKQIAAFARQVLGKTETADITIDEAMGAAQTFADQPFNGDGIVPADSASDDATKA
                     VINDIIDCLGGEKGTCGKLGVSQAKVDQFFAEAAAYAEWWKKAEGDSRILPLGLNTEA
                     AAAAVKGVKAKVDDFFARCRLAAFDPRSVNALNRDEKEFAALGAKDLTTTHADIASFP
                     LAQVGADQALPLTRGVNPAWADALAKLYADAVKPLLGDKTSLTEADWAALLAKLAPFD
                     AWSGSKAGGAVEKIGLARVRQILATKARETLTALIAKDKEKEGHAQAVIGLNRLVRYH
                     RDLGKLCNNFVNFRDFYGRKDKAIFQAGTLYLDQRSCDLCLTVEDAGKHATMAALAGT
                     YLAYCDCYRKGSGEKLQIVAAFTGGDSDNLMVGRNGIFYDRKGRDWDATITKIVDNPI
                     SIRQAFWSPYKKLVRMIEEMAAKRAAAAEASADAKLAETAQSVASGEAPKPAEPPKTK
                     FDTGTLAAIGLVFSTLLAALGGIFGKILGLSWWQIPLLFVLILLAISAPSMIIAWLKL
                     RRRNLGPILDANGWAVNAKAKINVPFGGSLTGVAALPPGSQRDLMDPFAEKKSPWPKI
                     ILVLIVLAIAGYFLFPKQRDCLLDRLGIGTKKPAKVEPAAPAPAAPTDATTQPADTEK
                     "
     gene            complement(13305..14564)
                     /locus_tag="PKI07_08795"
     CDS             complement(13305..14564)
                     /locus_tag="PKI07_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOC51188.1"
                     /translation="MRDLASSEDGEKPPSCSPAKALKAFRPAGRGFWANVPEAEWNDW
                     HWQLRQRITTVEQLQRLMPTLTPEELSGAKLANRKLALAITPYFFTLIDPADENCPIR
                     RQVIPRVEETYVAPWEMRDPCGEDAHSPVPGLVHRYPDRVLFLATDRCASYCRYCTRS
                     RLVSNASGYDFHPDYDRQIDYIRQHPEVRDVLVSGGDPLLFNDDKLAHLLGRLRAIPH
                     VEFLRIGSRVPIFLPQRITPELCAMLKRHHPLFISIHSNHPRELTTEAREALGRLADA
                     GIPLGNQSVLLRQVNDRPEIMTAHVQKLLMCRVKPYYIYQCDLVAGSAHLRASVRQGM
                     QIMEQLRGHTTGYAVPEYVIDAPGGGGKVPVAPEYVLSRNDERVIIRNHEGQIFEYPE
                     ASDGTPLKAPPPEKEELELEPAPAAAP"
     gene            complement(14689..16905)
                     /gene="priA"
                     /locus_tag="PKI07_08800"
     CDS             complement(14689..16905)
                     /gene="priA"
                     /locus_tag="PKI07_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOC51189.1"
                     /translation="MIARVTLEIALRKEFDYTIPPELAGQVEVGSRVQVPFGARKVMG
                     CVTGLAEESAQPRLRPLLKVVGTQSLVTPGILKLARWIGEYYCCAAETALKSVLPVAV
                     RREQANWRARLQVRATAPGEAPKLPRRQREVWQIVRARGELPLRELLALGKTTAGTVR
                     RLEDRGLVRITAEIAERDPYAGEHILPTQPLALNPAQAGALAALRAAMSGEARAATFL
                     LHGVTGSGKTEVYLQAIAHALEQGKGAVVLVPEIALTPQTVERFKARFSAGPQPTLVA
                     VLHSHLSAGERHDEWHKIRQGRARIAIGARSAIFAPVAPLGLVIVDEEHEHTYKQEEA
                     PRYNARDVAIMRGRMEGAVVVLGSATPSLESYHNCVKGKYRLLTLPERADGRKMPYVR
                     VVDMRQTARAEKGIPIFSPQLKEAIARRLEQKEQVILFLNRRGYSSSLQCPACGFVAE
                     CPNCSVSLTYHRQEQKLCCHICAHVAPVPAVCPNAHCRNPAIRYAGLGTQKVEAALAK
                     LFPQARAVRMDSDALKRKEDYRRILGDFRAGKIDILVGTQMIAKGLHFPNVTLVGIIY
                     ADLTLHQPDFRAGERTFQLLTQVAGRAGRGDVEGEVFVQALTPFVPAIQFARRHDFVG
                     FYQQEIEFRAQLNYPPLSRIALLTLKGRNEEKVKLSATHLKRELEKAAGELPGLVLSG
                     PAPAPLLRAETYYRYQIMLRARSMPALSRRLAGITQELPLPDGVALSVDIDPLDLA"
     gene            17135..17899
                     /locus_tag="PKI07_08805"
     CDS             17135..17899
                     /locus_tag="PKI07_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC51190.1"
                     /translation="MLLRFLLTLQGLGAFALITLGVMITRFRAARAVIRPLVFAEISR
                     AGLRLLPLFLFMAVALGLVVIGQTVSWLTRVGAINFLGTVMVVVVVRELGPLVTALLV
                     VARVGTANVIELGTARATGEVEALEALGIDPVHYLVVPRVLGMALGVYALTVYLILGA
                     LASGYLWAFVQDVPLLPGDYLRQLAGALSGLDFVLLAVKTFAFGAIIALVTCYHGLAQ
                     PLRLEEISRATVAAVAQSVVACVLLDALFIILYLAT"
     gene            17896..18690
                     /locus_tag="PKI07_08810"
     CDS             17896..18690
                     /locus_tag="PKI07_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC51191.1"
                     /translation="MTPPLPAPDEVPALEMINVAVGALHDPAIRVAENINWTVRPGDY
                     WVIAGLHGAGKSDFLRLAGGVMPPQQGQYRFFGEPMPIFEDARLPTRLRLGLVFDGGQ
                     LFNHLTLAENVALPLRYHRNLGHAEAAAAAAELLAGLELEPWAHRLPGAVGYGWRKRA
                     GLARALALQPEVLLVDNPLGGVDPRQVHWWLAFLDQLSRGHPLLRGRPVTLVVTAADF
                     PRWQGHARQFALLRDRRLRVLGGWGEVAAAREDAVRELLTKETQTG"
     gene            18702..19982
                     /locus_tag="PKI07_08815"
     CDS             18702..19982
                     /locus_tag="PKI07_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOC51192.1"
                     /translation="MALQDLTPQLRTRLSRMERLVGGFVTLAMALLTFGFAYYVYHTV
                     ERKGWFLTKASYYTYTDRATGLKVGDPVMLMGLAVGQITRMEPMPPEEFYHNIYVEFE
                     IKDPYYGYLWTQGSRAKVTTADLLGKRILEVTKGTGGYPTYIFYPLREVQPAEARALP
                     DPAHWLLAQEIWDESGTNLLAKAKTPLAELPAAALDACTNLLLMNTAEERKLMTGIWN
                     DREGRYDPCTRKTKPYWLLADESAAVTERLEKFMEEVERALPNILQLTNSLAAVLANS
                     TSLTSNLNNTALQTRPAISNLTALTARLNEPGALGEWLFPTNLHRQLEDTLATTDAAI
                     ARADFNLTALAENLGRSLDNLANLTHGLNDQIQANTNILSELSRAIVEADTFLQGLKR
                     HWLLRSAFRSKETNAPPPPRPAPPIRSPKGADER"
BASE COUNT         3505 a         6252 c         6319 g         4125 t
ORIGIN      
        1 taatagctgt tggcgttgta gccggccacc tcgaaggcga cgacgtttgc gcccggcttg
       61 agcttgtcgg tgaggtccca ctcgtccacc cggaaaaagc catgcgggcc gcgggcgggg
      121 ccgtgcgcca ggaactggcc gttaagaaaa acccggtaga tcgagctgcc cgtggcgcgc
      181 agcagaacct tgtcccgcgc gggcgcgttg aagctggcgc ggaatccgac ggtgatgttc
      241 ttctcggtct cgcgcccttt gggccagagc ggtttggcgg ccacaaagga cggcgtcgcg
      301 gcggcaaggt tcgcgcccgc cagcagcagg ccgagcaacg gcagcatcca ggccgccgcg
      361 cgggcagccg ctttgcaagg cgatccgata aggcaataag tcgtgggctt catgtcgtcc
      421 gcccattgaa gcctgaattg cgcccgggcc caattaaaaa aggtggcccg cgctttggcg
      481 tcccgccgca acgacggccc ggggtacaaa ggaagggggc ctccaggccc cctttctggc
      541 ttcgcgggaa accttcaacc aaaggctgaa ggggaagccg taggagcctt aactttattg
      601 cacggcccca ggttgtcaag gggggaaccc ggcagacgcg gcccggcggc ggctctcaag
      661 ttgcggcgga aacacgaccg agggaggagc cattgggcgc ggccaacgct ttgcgggcgg
      721 gattgcctgg cgattggctg acggttttac gggcgctggc gggtcgccca aaccggaaca
      781 attcagattt caatctcaat tcgcctccac agaacgtgta agcccccggt tttaagagcg
      841 gaaggcattc ggaatccggc ggccaacccg ttgaatccac caacccaaca gttccctgag
      901 cccctccgct ggcctgtcag gcccgttttg atcccagtag agcggaggca caggcttgaa
      961 ctgtgcaacg tcaacgtttc agataaaaca ttcgcgaagc caaaacccat ccgaacgccc
     1021 gtccgcccgt gaaaacccac tttttatcag ccttggttcc ataaccaact gtttttacgg
     1081 aacttagcaa taatcctccc atcccccctc ctcatttcgt cctttccagg cgcctatatc
     1141 tattatccag atcccttgaa aataccatac cttcaggccg tgtacaaggg gtgtacaagg
     1201 gatgcccatc gggagtccat aggcttttag cgggtacacc cgttgagcat cccttgtaca
     1261 cctcttgtac acggcctgga ggcagggtaa agccaaggtg gttccggggg atgccgaagt
     1321 cggcatgatt ctgcttctat tttacccctc tctatatcta aactattgat tttactgacg
     1381 aaaggatcgg gattcagcag ccatgccctt gaattcacta aataaagtgt tcgccggggc
     1441 tacggtttgg atggtcagga ttgcccaagc tccaataaaa cgagggagag tggttgagct
     1501 acccaactga atcgtctcgg ctgagctgcg gctgaccggt tggcgggtgg gcgcggttgg
     1561 gctgcgaggg cggggcggag gcgcttttcg gtcgttgcga ggaatctctg cttgctgcgc
     1621 cggagggttt attgctaaaa gggagcccgc aacaacatga cgccagctac taccgcttca
     1681 accgaacgca aacccgccgc gcccaggcat acctcccgca acccgtggct ttgggttccg
     1741 agcctctatt tttccgaggg cattccgtat gtgattgtca tgaccatgtc ggtggtcatg
     1801 tataagcgat tgggcatctc caataccgat atcgcgctgt acactagttg gctgtatctg
     1861 ccgtgggtca tcaagccgct gtggagcccg tttgtggata tcacgcgcac caagcggttc
     1921 tggattattg ccatgcagta tctggtgtcg ctcgggctgg cgctggtggc gttttcgatc
     1981 cagggcccgg cctttttcaa gtggtcgtta ttccttttct ggatcatggc gtttgcgtct
     2041 tccacgcacg atattgcggc ggatggtttt tacatgttgg ggcttaccaa gcatgagcag
     2101 gcgtggtggg tggggctgcg cagcacgttt tatcggacgg cgatgattgt cggcagcggg
     2161 ttgctggtgg tgctggcggg ggtgttggaa agcaagaatg ggttgccccc cgtcgagatt
     2221 cccgtgcagg ccagcacgca agcgccggtg gttgaggcgt gggatccggc ggcggttcgg
     2281 tttacggctc cggacggcga attgcgagtg ctggcgcagc cgaatccgct gccgattgcc
     2341 ctgacggagc ggagcgccga ggaggccagg gcggttatcg cccaggcccg ggcctggaac
     2401 cgccagcatg ggttcatcgc ggaggagaat cccgcgcctg ccaaggccga gtcggatgcg
     2461 ccgtcctggt ggacggcgaa tgtctccggc ccgttgggcg ggttgctgac gaattacttc
     2521 ccccgtccgc cccggagtgt ttcatcgcag gcgccagtcg cgggcaatgt cggcgtgctt
     2581 tatctttccc tgtcccagga accgcccgcg ggccaggaga ttgccgtcaa cttcggccgc
     2641 cagccggggg ggctggaata tatcggcctg ggtaagagcg acaagggttt cagccttaag
     2701 gaaggcgagc gttttgtgtt caacgccgcc aactggaatc agccggcgat ggccgtcatt
     2761 cagttggatc ccaagctcaa agctgacacg gccgtgacct tcgccaccag ttcgggcaat
     2821 attccgctgg cctggtctat cacgctgttt tttgtggcgg cgctgttcct ggctttcagt
     2881 ttctggcata ccgtgatgct gccccggccg gccagcgatg ggccggtggt cacgcatcac
     2941 agcctgttgg gcgagttctt tggcacgctg gggtcgttct tcaagaagcc gggcgttgtg
     3001 ctgtccatgg cgtttattct gctttatcgc tttgatgaag cccagttggt gaaggtgatt
     3061 tcgcctttcc tgctcgacag ccgtgaggcg ggcgggttgg ggttgacgac cagccaggtc
     3121 ggcgtggtct atggcacgtt cggcatcctg gcgctgacct tcgggggttt gctgggtggg
     3181 ttcatggccg ccaagcatgg tctgcggagg atgcttccca tcatggtcgc ttcgatgtat
     3241 ttgcccaagt tcgccttcgt ctttctgtcc tgggcccagc ccaccaactt tctcattacc
     3301 tgcgccgcgg tggcggtcga gcaattcggc tatggctttg ggttcaccgc gttcatgctt
     3361 tatatgctgt tctttgccga tggtccgcac aagacggctc acttcgccat ctgcacgggc
     3421 ttcatggcct tggggatgat gatcccgggc atgtggagcg gttgggtggc ggacctggtc
     3481 ggctacaagc atttcttcgt gtgggttatc tgctcggcta ttcccggctt caccctggcg
     3541 atgttgctct accgcagaat tgatccgcag tttggcaaga aaacatcgta gccatcttgc
     3601 ctggggcgga ggcgagtggt gcaattatga gggccgtgtt cccgagccgg gtgattggtt
     3661 tttttatttt tgccgggcgc gccacataca caacggaaaa aaactttttc acagccgtcc
     3721 tgtcggcaga gagtttccgg cggtggcagc ttaagttaag tgttgttttt ccaaatactt
     3781 gtagccagcg tgcgatttcg gttttttgcg ggaaggtggc ggcaaaagaa aagaaagaaa
     3841 gtggttgaca gaaaagtcgt tttggtgttt tttataggcg ctgtcgcaaa gtgatctttg
     3901 gaagctgcgg tgagagccgg gtgcccctgt tgggcggtgg gtaggatttt ccgatgcggc
     3961 aacttgcgag agcgcggtag acgattagac ctgatcagga atagttgagt tagagaagct
     4021 gcttatgaaa cgattaatga agagcgttgt gttgggcttg gtcggcgcga gtttgttggt
     4081 ggggacggcc agagcggaaa tctatatcac caatagcgtt gggcctttta cggatacgtt
     4141 ggcgcccgcc acgaccgagt ggaaccatgt ttttcaagtg caggctttcg atgcgcactt
     4201 ggggagtttg gtggctgttt atgtcgaagt gacagagaat gttgatgtca gcgggtttgt
     4261 gcagaacacc tccgcccaaa agcagagctt cttcttcagg tcaggctctg tgcttaccgt
     4321 gacgctgccc ggtgcgttgg gctttttgca acccaacccg ctggcgttga cgcagaccta
     4381 cacggatctt gcccccggcg caacagctcc gtatggtccc gcttctgcct cggaaagcgt
     4441 tgataatatg tatttcgccc cgctttctgg atggacatcc tatactggcc aaatggtgaa
     4501 gttcccggcc tacacgttga ccgatgaaag aatcacgggt ggcggcggaa atattgttgc
     4561 ggccatgaat acgattgccg gggcgacggt gagcgttacc tactactaca tcccggaacc
     4621 gggctcgctc gcgctgttgg cgttgggcgg ggcggcgctg ttgctgcgcc ggcgcaagta
     4681 gaagcgcact gagcgactgc caaagtcaag cttttcatta aaggccgtcc cgttgaacgg
     4741 acggcctttt aatttatcca ggggctttag ccgtctcatt ggcggcgtgg ataaagactg
     4801 aggacattgc ggagatcagg aagcttgggg gcctggttct gaaaggcgac gccctttgat
     4861 tcactctgcc actcgactaa cgaacggcgc acaagggcgg cgttctccga tgcggcggat
     4921 agttaccgga gttgtcctga tggattgaaa gcaaaaccgc cgcgcaggtg atagctacgg
     4981 ctgcgagcgg cgggtttgtg tttgccgaag ggctggaggt tagctggcgt agatggcgtt
     5041 cgaggccgta caggaacggg ttttaacgcc ggcgctccag cttatagcgg ggccagcaca
     5101 atccggaacc cggtatcctt tctggtgttg gttgcggcca ggttagcgcg ttgagccgag
     5161 cggcagtaac tgccgggata actgaagtag tttccacccc gaattacgcg atgcgccacg
     5221 ccggaggtgg gaccctgcgg atcggtgacc gtccccgtgg ggtattctcc ataccaatcc
     5281 tggcaccatt cgcacacatt gccgtgcata tcgtataatc cccagggatt ggctttcaaa
     5341 tagcccacag tgtgcgtcac gaccgaactg ttgcttttat accaggcata ggcggccagg
     5401 tttgaatagt cggggtcgtc tccgtagctg aatcgcgttg tcgtgccggc gcggcaggca
     5461 tattcccact ccgcttcggt gggcaaacgg tattgccatc cgggcgccag tcggcccgct
     5521 gccagttcct gccgggtgaa tttgccgcaa tagttggtgg cgtcatgcca gcttacggtt
     5581 tccaccgggc aatttagcgt gcattcggga taaccattgg ccggcgtgaa gtagctggga
     5641 ttgtttccca taatcgccag atattcggct tgccgcactg ggtatttgcc gatgtaaaag
     5701 ccccgagtca gcacgaccgt gtgctggtct tcgtccgcat accggccaac ttcattggtt
     5761 gggcttccca tcgtgaaggt gcccggcgaa atgtaaatca tgttggtcat caccaattgg
     5821 agcacggcgc gataaaaccg gttgccacag gcggggtttt gcgtatcgaa gtacaggtac
     5881 gggctgctgg gcagcgtaat aatggccagg tcctgccagg agtttggatt cgacaggtcg
     5941 gtggtggttt gaatggcgta cacgcagttg accaccccgg taatggtaat tccggcgtaa
     6001 aggtgcatgc cgacgcctgt ctgggccacc gccggcttgg gcactgcgca gaaactcaga
     6061 atgccgagca ggaagaacca ccggagcgtg cgcatggttt ggcggcaaaa cccctgacgc
     6121 ggcttcgggt gcgaagccgc cgggaatagt ggcgcggacg gggaaagagg gttgattttc
     6181 actgagacag cctgacttaa catatgtggc aactacgttt actgttattc ttcggacttg
     6241 ctcgaggagt cctggccaac aaggctgcct ctgactcaaa tcgttcaggg taattacata
     6301 gcacttttta agccaaacag caatgtgccg ccatctcctc atcccgcggt ttaggcgaac
     6361 gccgggcgga gcgcaaacat cccctttcgc ctgcgaaata gcgggcaaac cgtgaggccg
     6421 gagatgccgg cgtgcttgct ccggtttcgt gcccgcgtgg ggcgcgcgcg caaggtgcct
     6481 tggtcgaacg agacgctgtc cttaaacagg acatcttttc ttccaccccg cctgcagatg
     6541 gcgtgcgctg ggaggctgtt gttcgcggtg aggccggatt tgtgattgcg aatcgccgca
     6601 gataattggt agcgtagccg ggttggcaag agtttgacct gagctttggc cggttaatga
     6661 cgatggaaag agagcccacg cggccctgga actttttgac cacgggtcgg ccctggctgc
     6721 tggccggcgg tatgttggcg gtttacctgg cgaccctgag ctgcggtgtt acggcgggca
     6781 acctgcgctc agtggtcgaa ttaagcggct ggaactggca gccggaggtc gccgctccgg
     6841 tgacgttcct gctcacgtac cccctgcgtt ggctgccggc ccgcggtatt gccccggccg
     6901 caaacttgtg cagcgctctt tgcgcggctt tgaccctggc gctgctggcg cggtcggtgg
     6961 cgttgctgcc cgggcaaagg cggatttcgc ttctgagccc ccgcatggct tggctgccgc
     7021 cggccctggc ggtcttcgcc tgcggcctgc agttgacctt ttgggaaaac gccatccagg
     7081 cggctgggga aatcctggat ctcctgctct ttgccgccgc ggcgtggtgc gtgctggagt
     7141 ttcgggtcac gcgccagccg tcctggctca cccggtttgc ggttgggtac gggctggccc
     7201 tggcgaataa ttgggcgatg gccgggtttc tgccgctgtt tctggtggca tttctttgga
     7261 cccggccgct gcacctgctg gattttcgct ttgccgcgcg ggctttgcgt ctgggatgga
     7321 aggccgttgc gccggagctc acggcggatt ttcgcctttg cctgcgcctg gcgctgtgcg
     7381 ggctggcggg attgtcgttg ctgctgctgc tgcctttgct ggcgcacttt tcgaccaccc
     7441 ggcatttcga tttttggccg gcactgctct cggaactggg cgcctacaag cacatgctgt
     7501 ttcgctttcc caaaggcgtg attcttctgt taagcctgac ttcgatattg ccggcccttt
     7561 tcatgggcat aggccgctgg tcgctgcgct ttcaatcgga cagtcgcagc cccaagtccc
     7621 tggcggttcg aggcgtattt tacctcattc acgcagcatg gttgctggcc tgtatttggg
     7681 tggcgctgga ttgcccggtc agcccgcggc accgtggttt gggatttgcc tttcttccgc
     7741 tgtattatct gggggcattg agcattggct atttcagcgg gtattttctg ctggtctttg
     7801 gcgcgcgcaa ccccaatggc ctgccgcgac ggtctctcga aaaactggcg gcggcggcaa
     7861 ccgccggcat cgggctcctg ttgctgattg ttccaagcct gctgctttac cgcaacctgc
     7921 cgcaaatccg ggcaatccac cgcgatccct ggctgggata ttttacgctg gtggagaaat
     7981 ccctgcccgc gcaagggagc gtaatcctgt ccgacagttc cctgccgttg ctctttttcc
     8041 aggccgcgtt ggttcgcgac agcaagccat tcccgtattt gccgctcgat gccacggccc
     8101 tggacaagcc cgcttaccag gcgtttctcg gccgggcgta tccgcggttc gggctggccg
     8161 gcacgcagag caatctcttc gcggaatgcg ccagcccggt tggccgggtg cgcttgctgg
     8221 aagccctggc caagcggcac ggcctttatt atctccaccc cagtttgggc tactattttg
     8281 aagctttcta tccgcaggcg cagggcctgg tttaccaact gaagccgtac ggctccaatg
     8341 tggcaatcgc accgctcccg agcccggagt taattgcccg gaaccggcag ttttggcagg
     8401 aagctgaaac gaaccagttt gcagctcttg cccgggcgct gcagcaaggc agcccgccag
     8461 ccatcccgaa ttggattcag cgcctgctgg cgcgcattca tctgcagaac gagccgaacc
     8521 gcagcgccca gattgttggc gcccgttacg cgcgcactct taattactgg ggcgcggaat
     8581 tgcaggaggg gcgggcgtat tcggaagccg cgcaatactt ccgccaggcc agggaactga
     8641 atccggacaa cgtcgccgcg cgggtcaacc aggatttcaa cgcagaattg caggccgggc
     8701 gccctcccgt ccttgcgccc gccacggcca tcgaggaaga ggctggctac cggcgcaaat
     8761 gggaacaggt gattctggaa gacgggccgt tcgctgagcc caactattgt taccaggtcg
     8821 gcagcgcgct cgcccggaac gggctttatc accaggccct ccagcagttt aggcgggtgc
     8881 aaactctggc tcccgacctg acggatgccc gtttctggat ggcccggctg tttatccata
     8941 ccgggcatta ctcgaatgcg ctggcggcgg ttaatcagat tctgcaagcc catcccgacg
     9001 atgacaacgc cttgttcctc aaagcggttg cactgcttca gtccaaagcc tatgaggaat
     9061 ccctcgtccc ctttacctac ttgattaacc attaccccag taattacgcc gcgcagctaa
     9121 accgggcgat tgcgtatctc cagttgggcc ggctcgatgc cgcccgccgg gactacgagc
     9181 aagtggccca ggcccttccc cggtcctacc aggcctggtt cgggctggct gaaatcgcgt
     9241 atcggcgaaa ggatttcccc gcggcgatta cgaattatca gcgttacctg accaatgcgc
     9301 ctccggatac gcaagaggcc gggcaggtcc gcgcgcgctt ggaggaactc cagcgtaatt
     9361 cgccttgagc ttgattgtcg ggcgctccgg ccggcagcta ccgtttttgg tggtagtccg
     9421 cccgggagta tttctgcgcg gtcagcgcgc gcgtttcttc gagcagttgc gcgtcaggct
     9481 tgaactccgc ccaggccaga ccttgcccgg cagctcccgc cgcacgcatc gcctgctccc
     9541 aatccgcgcg ggcgagcgag aggtcccgcg gttggaccga tccttgaatg agcaagccgc
     9601 tgcgggtgcg gcgttgggcg gccccggcga ttttccggcc ctgccaaagc aaatctgccg
     9661 cttcgtgccc ttggaaacat tggccgggag cggtcgggtt gggcagcggg gctaattccg
     9721 tggcgaggcc gataagctgg aaagcggact gtatccattc gtggatgcgg cggtagctct
     9781 cccgggcaca gagcgcatac caaaagtggg atggcgggat ggccaggctg taagtccaat
     9841 cggcatcatg cggcaccaag ccgccgccgg tgggccgccg cacgagcggg cgtaaatgcg
     9901 tgaggcgctc gacgtcagca tatttctgga aatatccaaa tgaggcagcc ggttcgttcc
     9961 acccataaaa gcgcaggacg ggctggccca ggcgcggcgc agcgcgcagc agggcttcgt
    10021 ccagagccat gttggtggac ggatcggccg ggccggattg gagcaggtgc cagggttgag
    10081 cctgcattcg tgagggcgat ggaatgccgg gccggctagc ccgctgaggt ttgtttgcgc
    10141 cgcccgcctt gcgctggagg ggtcataagc agtgaacaat caaatcgggg tggtcactcc
    10201 aatccgcggg cacagtgccc gcagttcgca gccgcggcaa tcaggtttgc gcgcggtgca
    10261 gcggcggcgg ccatgccaga tcagccagtg gctgaactgg gtccactgcg cctgcggcac
    10321 caaagccatg agcgcttgct cgatcttttc gggatccttt tcctgcgtca agccgagtcg
    10381 ctgggccaag cgggcaacgt gcgtatccac gaccaccccg tagttgagat gaaaggcgtt
    10441 gcccagcacg acattggctg ttttgcggcc caccccatcc agttgggtga gggcttccat
    10501 tgtgtttggc acctggccgt catgccgctc caccagggcc tggccacagc ctttcaggct
    10561 ccgagccttg ttgcgataaa acccggtcgt tcggatatcc tgctccagtt cggggagcgg
    10621 cacccgggcg taatcggcgg cggagcgata ctttttgaag agcttggccg tgacgaggtt
    10681 gacccgctgg tcggtgcacc gggccgatag gatggttgcc accagcaatt cgagcgggtt
    10741 ggagtaattc agctcgcaat gcgcgttcgg gtaggctttg gccaggctgg ccataatctt
    10801 ccgcagtggc acgcccgggg catctttgat tttgcgaggc atggcggaag cgaggataaa
    10861 ctcattcaac cccggcggca aggtgccgcc ggggtgacga aatagagggt gcgcgtagaa
    10921 ctacttttcg gtgtcagccg gttgcgtcgt cgcgtcggtg ggggccgcgg gtgccggggc
    10981 ggccggctca acctttgccg gcttctttgt gccgattccg agccggtcaa ggaggcagtc
    11041 cctttgctta gggaagagga agtaaccggc gatcgccagc actatgagca ccaggatgat
    11101 cttcggccac gggcttttct tctcggcgaa cgggtccatg agatcgcgtt gcgagccggg
    11161 gggcagtgcc gctacacccg tgagcgagcc gccaaaaggc acatttatct tcgctttggc
    11221 gtttaccgcc caaccgttgg cgtccagaat cgggccgagg ttgcgccggc gcaattttaa
    11281 ccatgcgatg atcatggacg gcgcggaaat ggccagcagg atgaggacaa aaagcagggg
    11341 aatctgccac caggacagac cgagaatctt gccgaagatg ccccccaggg cggccagcaa
    11401 ggtgctgaac accaaaccaa tggcggccag ggtgccggtg tcaaacttgg tcttgggcgg
    11461 ctccgcgggt ttgggcgcct cgccgctggc tacgctttgg gcggtttcag ccagcttggc
    11521 atccgccgac gcctccgccg ccgcggcgcg tttggcggcc atctcttcga tcatccgcac
    11581 cagcttttta tagggcgacc agaatgcctg ccggatgctg atcggattgt ccacaatctt
    11641 ggtgatggtg gcgtcccagt cccggccctt gcgatcgtag aagataccgt tccggcccac
    11701 catgagattg tccgagtcgc ccccggtgaa ggcggcgact atttgcagtt tttctccgga
    11761 gcctttgcga tagcagtcgc aataagccag gtaggtgccg gccagggctg ccatggtggc
    11821 gtgtttgccg gcgtcttcga cggtaaggca aagatcgcaa ctccgttgat ccaggtacag
    11881 cgttccggct tgaaagattg ccttgtcctt gcgcccgtag aagtcgcgga agttgacgaa
    11941 gttattgcag agtttgccca gatcacgatg gtagcgcacc agccggttaa gcccgataac
    12001 ggcctgggca tggccttcct tttccttgtc tttggcaatc agcgccgtga gggtttccct
    12061 ggccttggtg gccaggattt gccgcacccg cgcgagccca atcttttcga ccgccccgcc
    12121 ggctttgctg ccgctccagg cgtcaaaggg ggccagcttg gcgagcaacg cggcccaatc
    12181 ggcttcggtg agggaagtct tgtctcccag caggggtttg acggcgtccg catatagctt
    12241 ggccagtgca tcggcccagg cgggattgac cccgcgggtg agcggcaggg cctggtcggc
    12301 gcctacttgc gcgagcggaa agctggcaat atcggcatgg gtcgttgtga ggtctttggc
    12361 ccccagcgcg gcaaactctt tctcatcgcg gttcaacgcg ttcacggacc gcggatcgaa
    12421 agccgccagg cggcagcgag caaagaaatc atccaccttg gccttcacgc ccttgacggc
    12481 ggccgccgcg gcttccgtgt tcagtcccag cggcagaatc ctggagtccc cttccgcttt
    12541 cttccaccat tcggcatacg cggcggcctc agcgaagaac tggtccacct tggcctggga
    12601 aaccccgagc ttgccgcagg ttcctttctc gccgccgaga caatcaataa tgtcgttaat
    12661 gaccgccttg gtcgcatcgt cgctggcgga atcggccggc acaatcccgt ccccgttgaa
    12721 cggctggtcg gcaaaggttt gggcggcgcc catcgcttcg tctatcgtga tatcggcggt
    12781 ttccgtcttg cctaaaacct gccgggcaaa ggcagcgatt tgtttgccct ccggagctgc
    12841 ctcgttgatt gccgcgagcc gcaaggtggg agagcctttg atcaggtcat ccggattctt
    12901 gagcaggccg ccggcccatt taacggccgc aatcagctcc ggcgcgcgca cgcggccgtc
    12961 tttatcggtg tcaatcaagg ccagggtggc ctggtcaaac tccagtccgg tagtcgggca
    13021 ggccagggcg acccacaact tctggtcgag ttgatccagc gccatcaggt cggcgcccgt
    13081 ttccagtttg acctggtcaa acccgccggc gcgaaagaac ttccacgagt gattagtatt
    13141 cataggcatt cgttaattat caggttaata caatgacacg attcacggcc tcaatttggg
    13201 cttacctgct caaaccctac ctgaaaccat tccggttggc cactgctaaa attgcgggcc
    13261 agatgccgcc ccacaggggg tggccgggga gggcgagacc ggcatcaggg cgcggccgcc
    13321 ggcgccggtt ccagttccaa ctcctctttc tccggcggcg gcgctttgag gggggtcccg
    13381 tcgctcgcct cgggatactc gaaaatctgg ccctcgtgat tgcggatgat tacgcgctcg
    13441 tcgttgcggc tcaggacgta ctccggcgcc accggcacct tgcctccacc gccgggcgcg
    13501 tcaattacat attccggcac cgcgtagccc gtggtatgtc cgcgcaattg ctccatgatt
    13561 tgcattccct ggcgcacgct ggcgcgcaaa tgcgcggagc cggccaccag gtcgcactgg
    13621 tagatatagt agggcttcac gcggcacatc agcagctttt gcacatgcgc ggtcatgatt
    13681 tcgggccggt cgttaacctg ccgcagcagc accgattgat tgcccagcgg aatgcccgca
    13741 tcggccagcc gccccagcgc ctcacgcgct tcggtggtca gctcccgggg atggttggag
    13801 tggatgctga taaacagcgg gtgatgccgc ttgagcatcg cgcacagctc cggggtgatg
    13861 cgctgcggca gaaagatcgg cacccgcgag ccaatccgca aaaactccac atgcggaatg
    13921 gcgcgcaacc ggccgagcaa gtgcgccagc ttgtcgtcgt tgaacaacaa cggatcgccg
    13981 ccgctcacca gcacgtcacg cacttcggga tgctggcgga tgtaatcaat ctgccgatcg
    14041 tagtccgggt gaaaatcata cccgctggca ttgctcacca ggcgcgaccg ggtgcaatag
    14101 cggcagtagg acgcgcaccg atcggtcgcc agaaacaata cccggtccgg gtagcgatgc
    14161 accaggcccg gcaccggcga atgcgcgtcc tccccgcagg gatcgcgcat ttcccagggc
    14221 gccacatacg tctcttcgac ccgcgggata acctgccggc ggatcgggca attctcgtcg
    14281 gccgggtcaa tcagggtgaa gaaataaggc gtgatggcca gcgccagctt gcggttggcc
    14341 agcttggcgc cggacaactc ctccggcgtg agcgtcggca tcaaccgctg aagctgctcg
    14401 acggtcgtaa tgcgctgccg taattgccag tgccagtcgt tccattccgc ctcgggcaca
    14461 ttggcccaaa atccacgtcc cgccgggcga aaagccttga gggcttttgc cgggctgcac
    14521 gatggcggct tttcgccgtc ctctgatgaa gctaaatcac gcatttttcg actaaagtcg
    14581 gattagcgca ctataatttt gatcggccac ctgtcaagga accctcggtc ggattttgtt
    14641 ttccggccgc ccggggccgc ggcggctggc ggcgcgggcc agcgcgcttc aagccaaatc
    14701 cagcggatca atgtccacgg acagcgcaac gccgtccggc agcggcaatt cctgcgtaat
    14761 ccccgccagc cgccggctca gcgcgggcat gctccgggcg cgcagcatga tctggtagcg
    14821 ataataggtt tcggcccgca acaacggcgc cggggccggg ccgctgagca ccagtcccgg
    14881 aagttcaccg gctgcctttt ccagttcgcg cttcaggtgg gtggccgaga gcttgacctt
    14941 ttcctcgttg cggcctttga gggtcagcaa ggcgatgcgg ctgaggggcg ggtagttaag
    15001 ctgcgcgcgg aactcaattt cctgttgata aaaccccaca aaatcatgcc ggcgggcgaa
    15061 ttggatggcg gggacaaagg gcgtcaacgc ctggacgaaa acctcgcctt caacgtcgcc
    15121 gcggccggcg cgcccggcca cctgcgtcag cagttgaaag gtccgctccc cggcgcggaa
    15181 gtccggctga tgcagggtaa ggtcggcgta aatgatgccg accagggtga cattggggaa
    15241 atgcaggccc ttggcgatca tttgcgtgcc gaccagaata tcaatcttgc cggcgcggaa
    15301 atcgcccagg atgcggcggt aatcctcctt gcgcttcagc gcgtcggaat ccatccgcac
    15361 cgcgcgcgcc tgcggaaaga gtttcgccag cgcggcctcg actttctgcg tccccaggcc
    15421 ggcatagcgg atggccggat tccggcagtg ggcattcggg cagacggccg gcaccggcgc
    15481 gacatgcgcg cagatatggc agcagagttt ctgctcctgg cggtggtagg tcagcgagac
    15541 gctgcagttg gggcattcgg cgacgaagcc gcacgccggg cactgcaggg agctggagta
    15601 gccgcggcgg ttcagaaaca ggatgacctg ctccttctgc tccagccgcc gggcgatcgc
    15661 ctctttaagc tgcggcgaaa agatgggaat ccctttctcc gcgcgcgccg tctgccgcat
    15721 gtccaccacg cgcacgtagg gcatcttccg gccatcagcc cgctccggca gcgtcagcag
    15781 ccggtacttg cccttgacgc aattatgata gctctccagc gagggcgtcg ccgagccgag
    15841 caccacgacc gccccctcca tccggccgcg cataatcgcg acgtcgcgcg cattgtagcg
    15901 gggcgcctct tcctgcttgt aggtgtgttc gtgctcttcg tccacgatga ccaggcccag
    15961 gggcgccacc ggcgcaaaga tggccgagcg cgcgccgatc gcgatccgcg cccgcccctg
    16021 ccggatctta tgccactcgt cgtgccgctc gccggcggat aaatggctgt gcagcaccgc
    16081 gaccagcgtc ggctgcggcc ccgcgctgaa gcgcgccttg aagcgttcca ccgtctgcgg
    16141 cgtcaacgcg atttccggca ccagcaccac cgcccctttg ccctgttcga gggcgtgggc
    16201 gatggcttgc agatacacct cggtcttgcc gctgcccgtg acgccgtgca acagaaaggt
    16261 tgccgcccgc gcttcgccgg acatcgctgc ccgcagggcg gccagcgccc cggcctgcgc
    16321 cgggttgagc gccagcggct gcgttggcag aatatgctcg cccgcgtagg gatcgcgctc
    16381 cgcaatttcc gcggtgatcc ggaccaggcc ccggtcttcc agccggcgca ccgtccccgc
    16441 cgtggtcttg cccagcgcca gcaattcccg caggggcaat tcgccgcgcg cccgcacgat
    16501 ctgccacacc tcccgctgcc ggcgcggcag cttcggcgcc tctcccgggg cggtggcgcg
    16561 cacctgcagc cgcgcgcgcc agttcgcctg ctcgcgccgc accgccactg gcagcacgct
    16621 tttcaacgcg gtctccgccg cgcagcaata atactcgccg atccaccgcg ccagcttcag
    16681 gatcccgggc gtcaccagtg attgcgtccc gaccaccttc aggaggggcc ggaggcgggg
    16741 ttgcgccgac tcttcggcca gcccggtcac gcaccccatc accttgcgcg ccccaaacgg
    16801 cacctgcacc cggctcccga cctccacctg gccggccagc tcgggcggga tggtgtaatc
    16861 gaactccttg cggagggcga tttccagggt gacgcgcgca atcatgttcg ttggtctcgg
    16921 gcgccgccgg gctttgcagg ccggcggccg ggtattctct accgctttgg gcaaaggttt
    16981 ccaatcccca aaatgggctt tgaccctcag ccgctctcaa ccctacgctg tcggcgtgga
    17041 aaacggaaat gccagtgcgc caacttcccc gtgcgcgcgc cggtggctgc ggcgccggcc
    17101 gtggccggga tcgccgaccc ggtattgggg cgggatgctg ctccgctttc tgctgacgct
    17161 ccagggcctc ggcgcgttcg cgttaatcac gctgggcgtg atgatcaccc gcttccgcgc
    17221 cgcgcgggcg gtgatccgcc cgctggtctt cgcggaaatt tcccgcgccg ggctgcggct
    17281 gctgcccctg ttcctcttta tggcggtggc gctggggctg gtggtcattg gccagacggt
    17341 ttcctggctc acgcgcgtcg gcgccatcaa cttcctgggc acggtcatgg tggtggtggt
    17401 ggtgcgcgaa ttaggcccgc tggtgacggc gctgctggtg gtggcgcgcg ttggcacggc
    17461 caacgtgatc gagctgggca ccgcccgggc gacgggggaa gtcgaggcgc tggaggcgct
    17521 gggcatagac ccggtgcatt acctcgtggt gccgcgcgtg ctgggcatgg cgctgggcgt
    17581 ctatgcgttg acggtgtatt tgatcctcgg cgccctggcc agcggctatc tctgggcctt
    17641 cgtgcaggat gtgccgctgc ttcccggcga ttacctccgc caactggccg gcgccctcag
    17701 cgggttggat ttcgtcctgc tggccgtcaa aaccttcgcc ttcggcgcca tcatcgccct
    17761 ggtcacttgc taccacggcc tggcgcaacc cttgcgcctg gaagaaatct cccgcgccac
    17821 cgtcgccgcg gtggcccaga gcgtcgtcgc ctgcgtcctg ctcgacgccc tgtttatcat
    17881 cctttacctc gcgacatgac gcctccgctc cccgccccgg atgaagtgcc ggctctcgag
    17941 atgatcaacg tggccgtcgg cgcgctgcat gaccccgcga tccgggtcgc cgagaacatc
    18001 aactggaccg tgcgccccgg cgattactgg gtcatcgcgg gattgcacgg cgcgggcaag
    18061 agcgatttcc tccggctggc cgggggcgtg atgccgccgc aacagggcca ataccgcttc
    18121 ttcggagaac ccatgcccat cttcgaggac gcccgcctgc caacccgcct gcgcctgggc
    18181 ctcgtgttcg acggcggcca gttgttcaac cacctcaccc tcgcggagaa tgtcgcgctc
    18241 ccgctgcgct accaccgcaa cctgggccat gccgaggccg ccgcggcggc ggccgaattg
    18301 ctggcgggcc tggagctgga gccctgggcc caccgcctcc cgggcgcggt gggctacggt
    18361 tggcggaaac gcgccggcct ggcccgcgcc ctggcgctgc agccggaagt gctgctggtg
    18421 gataacccgc ttggcggggt ggacccgcgg caggtgcact ggtggcttgc cttcctggat
    18481 caactgtccc gggggcaccc cctcctgcgg ggccggccag tgacactggt cgtcaccgcc
    18541 gccgatttcc cgcgctggca aggccacgcc cggcaatttg cgctcctgcg cgaccgccgc
    18601 ctccgggtgc tgggcggctg gggcgaagtg gcggccgccc gcgaggacgc cgtgcgggag
    18661 ttattgacga aagaaacaca aaccggataa tcccccacgg aatggcgctc caggatctga
    18721 caccgcaatt gcgcacccgc ctcagccgca tggaacggct cgtgggcggg ttcgtgaccc
    18781 tggcgatggc gttgctgacc tttggcttcg cctattacgt ttatcatacc gtcgagcgca
    18841 aaggatggtt cctcaccaag gcttcctatt atacctacac cgaccgcgcc accggcctca
    18901 aagtcggcga ccccgtgatg ctgatgggcc tggccgtcgg ccagattacc cgcatggagc
    18961 cgatgccgcc cgaggagttc taccacaaca tctacgtcga attcgaaatt aaggacccct
    19021 actacggcta cctctggacc caggggtcgc gggccaaagt caccaccgcc gacctgctcg
    19081 gcaaacgcat cctggaagtc accaaaggca ccggcggcta ccccacctac atcttttatc
    19141 cgctgcgcga agtccagccc gccgaagcgc gcgccctgcc cgaccccgcc cactggctgc
    19201 tggcgcagga aatctgggat gaaagcggca ccaacctgct ggccaaagcc aagacgccgc
    19261 tggccgaatt gcccgccgcg gccctggacg cctgcaccaa cctgctcctc atgaacaccg
    19321 ccgaagaacg gaagctcatg accggcatct ggaacgaccg cgaaggccgc tacgacccct
    19381 gcacccgcaa aaccaagccc tactggctgc tggccgatga atccgccgcc gtcaccgaac
    19441 ggctcgaaaa gttcatggaa gaagtcgaac gggcgctgcc caatatcctg cagttgacca
    19501 actcgctcgc ggccgtgctg gccaacagca ccagcctcac ctcaaacctg aacaataccg
    19561 ccctgcagac ccggcccgcc atcagcaacc tcaccgccct caccgcgcgc ctcaatgagc
    19621 ccggcgcact gggcgaatgg ctctttccca ccaatctcca ccgccaactc gaagacaccc
    19681 tcgccaccac cgatgccgcc atcgcccggg ccgacttcaa cctcaccgcc ctggccgaaa
    19741 acctgggccg ctccctcgac aacctggcca acctgaccca cggcctgaac gaccaaatcc
    19801 aggccaatac caacatcctc agcgaactct cccgcgccat cgtggaggcc gacacctttc
    19861 tccagggcct caagcgccac tggctcctcc gctccgcctt ccgctccaaa gaaaccaacg
    19921 cgccgcctcc gccgcgcccg gccccgccca tccgctcccc caaaggcgcg gacgaacgct
    19981 aacttcaaat tacgcggccg gaaccacggg ccgggcgagg ttaagcgggc ggattaaccg
    20041 gattcccgca cccgccagaa accatgccgc agggggagtt ttgactgctg agccggaccg
    20101 attcagattt cgcctccaat tcgaccccac agatgctcaa gtccccggtt ttaagggtta
    20161 aagacattcg gaatcccgcg gccaaaccgt tgaatcccta a
//
LOCUS       DAOPQG010000067        19974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3332_length_19974_cov_15.423616, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000067 DAOPQG010000000
VERSION     DAOPQG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 19974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19974
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_19974_cov_15.423616"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..2203
                     /locus_tag="PKI07_08820"
     CDS             95..2203
                     /locus_tag="PKI07_08820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOC51193.1"
                     /translation="MPYQMLSLLLLPGLLIVCVGMAEPTPPADRLKEFRERAAAFNAV
                     ITLPQFETTPDQIRLNLRETIARGNAALDQLGRLKPGEVTFENTVRALDDIGYEINLT
                     GDRFGLIKETSTNAALREAATDALKELQEWAVGLDYREDVYQAIKAYAHRQPKLEGEA
                     AKLLFETMRDYRRAGLDLPKPQRDEVERLRKELTRLMTDYESNITRAQKDVKFTRAEL
                     EGVPESFLAQAKTGADEYTVKANVTWHLLSVMDNARREETRKRLQFERDNLARESNLP
                     LLEKILPLRDNIARQLGYPSWADYRIEVKMATNAATAMDFLEKLKAGLQPKFDAELAE
                     FRRLKIKETGDPNARVNLWDWRYLSNQLKKEKYNVDAEQLRVYFPYQRVLDGMFRIYQ
                     SIFGLRFEPVEPPQKWIGDLQLYAVADAQSGEPLGLFYLDMFPREGKYHHFAQFHLVG
                     GKRLASGKYQRPTVALICNFPSPAPDKPSLLSHSDVETIFHEFGHAMHSILTRAQYSR
                     FAGTSVPGDFVEAPSQMLENWVWDKKVLDSFAADYRDPEKKIPADILAKLREAKLATE
                     ATRYRRQLSFGLTDLTLHTQIRAGNAAQTLTLANQVLSDVFLPMMPDTAFVAYFGHLM
                     GYDAGYYGYAWADAIAADMATVFEHAPKGYFDTTAGRRLRTEIYEPGNSRDVNVSIEK
                     FLGRKPSLAPFLRKLGIESK"
     gene            complement(2239..3201)
                     /locus_tag="PKI07_08825"
     CDS             complement(2239..3201)
                     /locus_tag="PKI07_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HOC51194.1"
                     /translation="MQALRRAVIDVGTNSVKLLVADLAGPEIRRVCEESRQTRLGRGF
                     YETHRLRADAIAATTAAVAAFAARARKAGAASIRVIATSAAREAVNRAELLAAIAQAT
                     GLRVEVISGDQEADWGFAGVSTDPQLGSAPVLILDVGGGSAQFIFGAGGKRFYRQSFA
                     LGAVRLRETLPCHEPPRAEELAATRRWLQRFLQAEVMPPLAAARAARRDETAGAVQLV
                     GVGGTASVLGGMEAELATFDPARLEATRLSAARVTWHVERLWGLPLPQRRRIIGLPPN
                     RADIILMGAAIFEAVMQAGGFGELRLSTRSLRFAALRPDPAPAA"
     gene            complement(3173..3478)
                     /locus_tag="PKI07_08830"
     CDS             complement(3173..3478)
                     /locus_tag="PKI07_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOC51195.1"
                     /translation="MIIGTVRGEIVSTINHPFYTGRKLLVVEKEDPAGRPGGYLIAID
                     GGVSAGVGERVLVIDEGGSARQILGDSEAPLRSIIVGVIDRIDLAADSHASPPPRGH"
     gene            complement(3475..3747)
                     /locus_tag="PKI07_08835"
     CDS             complement(3475..3747)
                     /locus_tag="PKI07_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOC51196.1"
                     /translation="MLLGRVIGTLTPGVVYPGLAGVPLLWVQPLDPHGRPRGEPLVAC
                     DATRAAGPGELIYYEGGREAALALEISFVPVDHTIVGIVDDLHLPA"
     gene            complement(3747..4361)
                     /locus_tag="PKI07_08840"
     CDS             complement(3747..4361)
                     /locus_tag="PKI07_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOC51197.1"
                     /translation="MKKHPALALIEFSSLADGLYCTDAVLKKAPIAMLKSGTVSRGRY
                     LVILGGSTAAVEESLHAALAAAPASLLDHAFLPDAHDQVHDALLGLRQPVIPDALAML
                     ETDTVAANVRAAEQALKGARVRLTELRLAEYEMSGKALSLFNGALHEIEAAMELAGAF
                     LRGRKEYIRHRIIARPHEALLRQLDRGTRFAEAQPLALEGEEVS"
     gene            complement(4361..5800)
                     /locus_tag="PKI07_08845"
     CDS             complement(4361..5800)
                     /locus_tag="PKI07_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOC51198.1"
                     /translation="MGGLSDQEIEAIARQIVAGLAGDGPPRPDRPARPSVAGELGVFD
                     HLDAAVRAARDAFIQLSGLGLQKRNVLLAAMRDAMREHGRALAREAHEETGLGRYEDK
                     VLKNQLVTEKTPGTEDLQPQTVTGDHGLTLTEPAPYGVIGAITPTTNPTSTIINNALG
                     MIAAGNAVVFNVHPNARRVSCHTVALLNKAILAAGGPRHLITCLATPTLESAQALMRH
                     PGIRLVVVTGGGGVVKAAMESGKRAICAGPGNPPVVVDETANLDQAARDIVFGASFDN
                     NIICADEKACLVAAPVADKLIQGLIYHGAALVWKDQLPALEKVIFRKTRGPRAEAEME
                     RALVGQDASVILRKAGIACADTVRLGIVEVGPDHPLLWTEQMMPILPLTRVRDADEGI
                     DLAIALEHPRRHTFVMHSDNLSHLSRMARECDASIFVKNGPALAGLGWQGEGHTSFTI
                     ASPSGEGMTSPRSFSRWRRCALVGAFRIV"
     gene            complement(5806..6096)
                     /locus_tag="PKI07_08850"
     CDS             complement(5806..6096)
                     /locus_tag="PKI07_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOC51199.1"
                     /translation="MTLGKVVGTVVATRKEATLDGLKFMVVRAVDPEGRETGGHVVAA
                     DAVGAGPGEMVLIATGSSARQTVTTQNRPCDAVIMAIVDSWEIGGVDKYRKD"
     gene            complement(6181..6477)
                     /locus_tag="PKI07_08855"
     CDS             complement(6181..6477)
                     /locus_tag="PKI07_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOC51200.1"
                     /translation="MAEALGLLETRSFAAMVEGADAMVKAAKVDLVSFEKTGGGYVTA
                     VIRGDVAAVKAATDAGRLAASRVGEMVAVHVIPRPHANVDEVLPLGRGAKAIKK"
     gene            complement(6530..7168)
                     /locus_tag="PKI07_08860"
     CDS             complement(6530..7168)
                     /locus_tag="PKI07_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51201.1"
                     /translation="MMADLRSYIFLDRLQPQVASFLATVSQGFLPIAGQASLLVEISP
                     GMEINFVTDVALKCTKVTPGMQIIERLYGLLEIHSESQADVRSAGAAILAALGLKEED
                     RYKPAIYSRSTIRQIDDHHTMLINRTRHGNMILPGQSLFTMEVAPAAYAAFAANEAEK
                     TAEINILEIRPFGSFGRLYLGGEEKDIDVAAQAAEAAIHSLAGRPMTGGGGA"
     gene            complement(7189..7878)
                     /gene="deoC"
                     /locus_tag="PKI07_08865"
     CDS             complement(7189..7878)
                     /gene="deoC"
                     /locus_tag="PKI07_08865"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOC51202.1"
                     /translation="MNIPALIDHTILRPDTTRADIEQLCREARQYQFYSVCVNPTWVA
                     LARQLLDGSGVKVCCVVGFPLGAQPPETKAMEARAAIRQGAREIDMVLNVGALKSGDD
                     ALVLRDIRAVVEACRDGSARCKVILETALLNHPEKVRACELAIQARADFVKTSTGFSS
                     GGATVEDVALMSRIVRAKGLGVKASGGIRTLADLRRMVQAGATRIGTSSGLKILQEAA
                     GEAVPGGGETY"
     gene            complement(8059..8481)
                     /gene="rpiB"
                     /locus_tag="PKI07_08870"
                     /pseudo
     CDS             complement(8059..8481)
                     /gene="rpiB"
                     /locus_tag="PKI07_08870"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
     gene            complement(8712..10277)
                     /locus_tag="PKI07_08875"
     CDS             complement(8712..10277)
                     /locus_tag="PKI07_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC51203.1"
                     /translation="MSDRAFFRRQFWLVAVLLAGTGLQPVSGTHAPRGEYAETHLLVK
                     LKAEKLPLLSTQSETEALTTLAAGLGLPSEARLEEPPVRRLLRSRSRRGAGPGTEAPV
                     DVSRFLRLHLPPGMRVTNALQQLSRHGWVEYAEPDGIGTGGVIPSDPDILRQWHHTNS
                     VKPTANIHTWQAWNITQGSPAVIVAMLDTGLNGDQPEFAGRVVPGYRFTNPACNTDTA
                     DDHGHGTMAAGILCASANNGALGAGVDWHCRVMPIKVLDRNNSGYYSDWAQGIDYAVA
                     QGAKVINLSAGGASSSITLSNAIARAIAQGVIFVTIAHNDGTNAIRFPGNLPICITVG
                     ATDRNDVRAGFSNYGLEIDLVAPGVGITSVSRSGTLATWSGTSFAAPQVAGVCSLLAA
                     VRPGLTQSEARALLRAGADDQVGDAADTPGFDPYYGWGRLNAGNTLLLAQTRIDSIWR
                     LPNGQVVLSWPSPPNAGSRQPYRIRAKSSLTGAWMQQTSPFGFGYSTNRTYWTNTNTK
                     ASVGFFEVKVNFP"
     gene            10920..12218
                     /gene="hflX"
                     /locus_tag="PKI07_08880"
     CDS             10920..12218
                     /gene="hflX"
                     /locus_tag="PKI07_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOC51204.1"
                     /translation="MKALVENRNHRTERVFLIGVELKSRRGAEVDDSLKELAELAATA
                     GGSVVGDGLQKVAAPCAATYIGKGKAEEFARYCQQAEVDTVIFDDELTPAQSRNLERV
                     FGCKILDRTALILDIFAARARTREGKLQIELAQLRHLLPRLTRFWGHLSRQKGGIGMR
                     GGEGETQLETDRRRVQDRIARLARELEVVRRQRSLQRNQRQRNHWALASIVGYTNAGK
                     STLLNALTGATVLTEDKLFATLDPTTRRLRLPTNQNVLVTDTVGFIRKLPHGLVAAFK
                     ATLEEVVQADLLLHVVDASHPDAEAQIQAVNAVLREIGADGKPTVMALNKLDQLAERP
                     EVLAGFQERYPHAAAISAVTGEGIPALLAELSSQLRPLREFMELSVPHEQAGVIARLH
                     EVGQVVERDYNGQTARFKARIPPHLHDEFAPFLVRELQTA"
     gene            12326..12988
                     /locus_tag="PKI07_08885"
     CDS             12326..12988
                     /locus_tag="PKI07_08885"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018144928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOC51205.1"
                     /translation="MKSRRAHIRRATNETRIAIELNIDGTGRSVIHTGIPFFDHMLTL
                     FARHAVVDLKLRGQGDLEVDAHHTVEDCGLALGQAFVLALGDKKGIRRYGTGFDPRNP
                     FTGEAYIPMDECLARCVIDFGGRPCLVWRGMAALAYKRVTRAEREQDMSSAFRFGLAR
                     EFFQGFTNEARCNLHLELLYGDEPHHIVEALFKAFAKAVDAACRRDPRMAGQLPTTKG
                     KL"
     gene            13028..13627
                     /locus_tag="PKI07_08890"
     CDS             13028..13627
                     /locus_tag="PKI07_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC51206.1"
                     /translation="MTVKDYSSAEERALVRMAQQGDLAAFEELVARHRDKIYARAYSM
                     MRHEQEALDLAQEAWVKGWQRLGQFQGDAGFGTWLTRIVINLCLDALRRQKRQRVESL
                     EALAEESGGVERQMPVVVPNPTAGLERAELRQRIDQALGQLSHEHRTVLILNEFEEMD
                     YKEIARTMGCSIGTVMSRLFYARRKLAALLADLKKPDTT"
     gene            13627..14106
                     /locus_tag="PKI07_08895"
     CDS             13627..14106
                     /locus_tag="PKI07_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51207.1"
                     /translation="MDLAAQLKLQAWLDGELPEAEARAVADWVAREPEASALAEELRQ
                     TRRALAGFEADLRLPESREFFWSKIEREIARRAAPAPASAPWGAAILAGLRRFLIPAT
                     AAALLVLVGAVLIRPGGAPTRGGGAEVEMALADAGAFTYCDYSAGTTLVWFSYPADN"
     gene            14167..14613
                     /locus_tag="PKI07_08900"
     CDS             14167..14613
                     /locus_tag="PKI07_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51208.1"
                     /translation="MAPWVWVSLALALLASAGFGAPPADMKLQAYLLWGTNDRNPPAD
                     RNYQPVNPDIRQKLKSLPLKWAHWYEVKRVDFQVAPGASREVTLSDKCQVKLRQLASA
                     EVEVLLIGQGKEVMKRRQALPKGDLLVLGGNSPNATAWLVVLKRNE"
     gene            14819..16399
                     /locus_tag="PKI07_08905"
     CDS             14819..16399
                     /locus_tag="PKI07_08905"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOC51209.1"
                     /translation="MHDWINYRSERKRSLWKALVFLAGAAVIGLAPSALARKPSYQLW
                     AVSPLTKVLQDQARPTNAWDRIALYAAKGESEAGQVVISAGSEPLKDVRVSLEPLTGP
                     GGAITQIELSKVVYINLPNLKKIVPDALPPATPFEVAAHSNQPIWITLIIPRDTRPGV
                     YSGKLVVKPANAAKQRIPIRLKVWNFTLPETPSHKTAFGISRNLLPRQHGVLKGSEAA
                     EQLYARYYNFLLDRWISAYWLPPAVTAPEAERYIRDPRVTGFLIPYNDDVEEMRKSVQ
                     SLRRRGLLDKAYFYPVDEPYTEPHYKRLVEVCTKIHSIDPKLRITIPYYRDPSFDTGG
                     KTLHELADGLINIWCPNIWYFNENKERLFAKLAKGEEFWWYVCCDPAPPYPNYFLNLD
                     GPSHRVLPWMGWKYRCQGLLYWNTTWWDEGKNGTEDPWTDMATVKGINKNMYGDGSLL
                     YPGKKVGVDGPVSSIRLELLREGLQDYEYLVKLEQKIGRAATEKFVSQLVTNPMDFNH
                     NVDDWVRVRVAIGEELGK"
     gene            16519..17262
                     /locus_tag="PKI07_08910"
     CDS             16519..17262
                     /locus_tag="PKI07_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51210.1"
                     /translation="MAVLAGLAVGVLNFLQVKEKITVLQADLERETALHHEFEGKYQT
                     TRKELDVCSKELKNTKDELATTVAARDKAEADLQAQTKRADKLTEDLNKTRQERNDAQ
                     AELAAYKVTGVTPQQILAMNKQFKSLQDNLQVAEAENKLLNQKIKKLDTELSVYRDPN
                     FFVSLPAALKGKVLVADPKWNFVVLNIGEDQGVLERGELLVNRNGQLVAKVVVRSVQK
                     DRCIANVLPGWELGQVMEGDVVIPAHPSS"
     gene            17259..17438
                     /locus_tag="PKI07_08915"
     CDS             17259..17438
                     /locus_tag="PKI07_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51211.1"
                     /translation="MKAAVLRLRWGLLLLAVLAAVVGGGCATTESENAAETPWNRPQG
                     WETGLPGGMYNPYPR"
     gene            complement(17471..18073)
                     /locus_tag="PKI07_08920"
     CDS             complement(17471..18073)
                     /locus_tag="PKI07_08920"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOC51212.1"
                     /translation="MNSAQAIQEAKQALRRQVRAALARLGPEERAAGAAQARARLAAQ
                     PQWQAAQWVLFFAPLPQELDVWPLLPEALAAGKNVALPRFAAATQTYEVCGIQDAAAD
                     LIMGRFGIREPGPRCARLAARRLDLILVPGIAFDVRGARLGRGKGYYDRLLKEWPGTT
                     CGVGFDLQLRNEIPCEPHDARLDCLLTPTRWIERPPLSPA"
     gene            complement(18081..19466)
                     /locus_tag="PKI07_08925"
     CDS             complement(18081..19466)
                     /locus_tag="PKI07_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOC51213.1"
                     /translation="MSRDDLFSIAPGAAPADAGAPESGAERHQPLAARMRPRDLGEFV
                     GQAHILGPGQLLRRAIEADRIQSLIFYGPPGTGKTSLAQIIARQTRNKFERLSGVESN
                     VADMRRVLAAAANRLANKHQSTILFIDEIHRFNKAQQDVLLPDVETGVVRLIGATTHN
                     PFFFVNSPLVSRSQIFELRPLAEAALLELLQRALADPERGLGYLKVQAEEAALRHLAK
                     LSDGDARKALNALEIAVLTTAPEADGVIRLDLAVAEQSIQKKAVVYDDEDAHYDTISA
                     FIKSMRGSDPDATLYWLAKMIHAGEDPRFIARRIVIHAAEDVGLADPMALVLANAAFQ
                     AAEFIGWPEARIPLAEAAIYIATAHKSNRALLAIDAALEDVATGRTLAVPEHLREAGY
                     RGAQRLGRGEGYQYAHDHPGHFVVQDYLGAHKRYYEPTEQGVEKKIKDRLAHWRALQA
                     QAQARDPEAKG"
BASE COUNT         3399 a         6508 c         6735 g         3332 t
ORIGIN      
        1 ctcaagcgct gatgccaata ggcccaaggt tcgggttcgg gcggcgccgg tttcctcttg
       61 catctgcgcg ttctggcatt aggttgcgcg ccgtatgcct tatcaaatgc tttcgttgct
      121 gctgttgccc ggcctgctga ttgtatgcgt tggcatggcc gagccgaccc ccccggcgga
      181 tcggttgaag gagtttcgcg agcgcgccgc ggcctttaac gcggtcatca ccctgccgca
      241 attcgagacg acgcccgatc aaatccgcct gaacctgcgg gaaaccatcg cccgcggcaa
      301 cgccgccctg gaccagcttg gccgcctgaa gcccggcgag gtcacctttg aaaacaccgt
      361 gcgcgccctg gacgatatcg gctacgagat caacctcacc ggcgaccggt ttggattgat
      421 caaggaaacc agcaccaacg ccgccctgcg cgaagccgcc accgacgcgc tcaaggaatt
      481 gcaggagtgg gccgtcgggc tggattatcg cgaggatgtc tatcaggcga tcaaggctta
      541 cgcccatcgc cagccgaagc tggagggcga ggcggccaag ctgctgttcg agacgatgcg
      601 cgattaccgc cgcgccggcc tggacctgcc caagccccag cgcgacgagg tggaacggct
      661 gcgcaaagag ctgacgcgcc tgatgaccga ctacgagtcc aacatcaccc gggcgcagaa
      721 ggacgtgaag ttcacccggg ccgagctgga gggcgttccg gaaagctttc tggcgcaagc
      781 caaaaccggc gcggatgaat acaccgtcaa ggccaacgtc acctggcacc tcctgagcgt
      841 gatggacaac gcccgccgtg aggaaacccg caaacggctc cagttcgagc gcgataatct
      901 cgcccgcgag agcaacctgc cgctgctgga aaagatcctg ccgctgcggg ataacatcgc
      961 ccgccagctc ggctacccgt cctgggcgga ttaccggatt gaagtcaaaa tggccaccaa
     1021 cgcggccacg gccatggact ttctggagaa actcaaggcc ggtctgcagc ccaagttcga
     1081 cgcggagctg gcggagttcc gccgcctcaa gatcaaggaa accggcgacc ccaacgcgcg
     1141 cgtcaacctc tgggactggc gctacctcag caaccagctc aagaaggaaa agtataacgt
     1201 ggacgccgag caactgcgcg tttatttccc ttaccagcgg gtgctggacg gcatgttccg
     1261 catctaccag agcatctttg gcctgcggtt cgagccggtc gagccgccgc aaaaatggat
     1321 tggcgacctg cagctttacg ccgtcgcgga cgcccaaagc ggcgaaccgc tggggttatt
     1381 ctacctggac atgttccccc gcgaaggcaa ataccatcac ttcgcccagt tccatctcgt
     1441 gggcggaaaa cggctggcgt cggggaaata ccagcggccc accgtggcgc tgatttgcaa
     1501 ttttccgtcg ccggccccgg acaagccctc gctgctgtcc cacagtgatg tggagaccat
     1561 tttccacgag ttcggccatg ccatgcactc gatcctgacc cgcgcccagt atagccgctt
     1621 cgcggggacc agcgtgcccg gcgacttcgt cgaggccccg tcgcaaatgc tggaaaactg
     1681 ggtctgggac aaaaaagtgc tggatagctt cgcggccgac taccgcgacc cggagaaaaa
     1741 aataccggcg gacatcctgg ccaagctccg ggaagccaag ctcgccaccg aggccacccg
     1801 ctaccgccgc cagctttcgt tcggtttgac cgacctgacc ctgcacacgc aaatccgcgc
     1861 cggcaacgcc gcgcaaaccc tgacgctggc caaccaggtg ctgagcgatg tctttctgcc
     1921 catgatgccg gacaccgcat ttgtggccta cttcgggcat ctcatgggct atgacgccgg
     1981 gtattatggc tacgcctggg cggatgccat cgcggcggat atggcgacgg tcttcgagca
     2041 cgcgcccaag ggctacttcg ataccaccgc cggacgccgc ctgcgcacgg aaatctatga
     2101 gccgggcaac tcccgggatg tgaacgtgtc catcgagaag ttcctgggcc gcaaaccctc
     2161 cctcgcgccg ttcctgcgga agctcggcat cgagtcgaag taacgcgcgc ccccgcgccg
     2221 ccgccgggcg ttggccgctc aagccgccgg cgcggggtcc gggcgcagcg cggcgaagcg
     2281 caggctgcgg gtgctgaggc gcagttcgcc gaagccgccc gcctgcatga ccgcctcgaa
     2341 aatggccgcg cccatcagga tgatgtcggc gcggttcggc ggcaggccga tgattcggcg
     2401 gcgttgcggc agcggcagcc cccagaggcg ctccacgtgc caggtcaccc gcgcggcgct
     2461 caggcgcgtc gcctccagcc gcgcgggatc aaacgtggcc agttccgcct ccattcctcc
     2521 cagcacgctc gccgttccgc cgacccccac taattgcacc gcgccggcgg tttcatcccg
     2581 ccgcgccgcc cgggcggcgg ccagcggggg catcacttcg gcctgcaaaa agcgctggag
     2641 ccaccggcgg gtggccgcca gttcctccgc ccggggcggt tcgtggcacg gcagcgtctc
     2701 ccgcagccgc accgccccca gcgcgaagct ctggcggtaa aaccgttttc cccccgcccc
     2761 gaagatgaat tgagcgctgc ccccgccgac atccaggatg agcacgggcg ctgagccaag
     2821 ctgcggatcc gtgctcactc ccgcaaaccc ccaatccgcc tcctggtcgc ccgagatgac
     2881 ttccacccgc agcccggtgg cttgcgcgat ggccgccagg agttccgcgc ggttgaccgc
     2941 ctcgcgcgcg gcgctggtgg caatcacgcg aattgaagcc gcccccgctt tccgggcccg
     3001 ggccgcaaac gcggccacgg cggcggtggt ggcggcgatg gcgtcggccc gcaggcggtg
     3061 cgtctcgtaa aagccgcgcc ccaggcgggt ttgccggctc tcctcgcaaa cccgccgaat
     3121 ctccgggccc gccaggtcgg ccaccagaag ctttacggag ttggtgccga cgtcaatgac
     3181 cgcgcggcgg agggcttgca tgggagtctg ccgccaggtc tatgcggtca atgacgccga
     3241 caatgatcga gcggaggggg gcctcggaat cgccgaggat ctggcgggcg gagccgcctt
     3301 catcaatcac cagcacgcgc tcgccgaccc cggcgctgac gccgccgtca atggcgatga
     3361 ggtagccgcc gggccggccg gccgggtctt ccttctcgac caccagcagc ttccggccgg
     3421 tgtagaacgg gtggttgatc gtcgagacga tttctcccct gacggtgccg atgatcatgc
     3481 cggaagatgg aggtcgtcca cgatgcccac aatggtgtga tccacgggga cgaatgatat
     3541 ttcgagcgcc agggcggctt cgcggccgcc ttcgtagtag atcagctcgc cggggccggc
     3601 ggcgcgggtg gcgtcgcagg cgacgagcgg ctccccccgg gggcggccgt gcgggtcgag
     3661 cggctggacc cacagcaggg gcacgccggc gaggcccggg taaacgacgc ccggggtgag
     3721 cgtgccgatg accctgccca gcagcatcag gagacctcct cgccctcgag cgccagcggt
     3781 tgggcttcgg cgaaccgggt gccgcggtca agctgccgca gcagcgcctc gtgcgggcgg
     3841 gcgatgatgc ggtgccggat atattccttc cgtccgcgca ggaaggcgcc ggccagctcc
     3901 atggcggcct cgatttcatg caaggcgccg ttgaagaggc tgagggcctt gccggacatc
     3961 tcgtattcgg cgaggcgcag ttccgtcagc cgcacccggg cgcccttgag cgcctgttcg
     4021 gcggcgcgga cgttcgccgc cacggtgtcc gtttcgagca tggccagcgc gtccgggatg
     4081 acgggctggc gcaggccgag gagggcgtca tgcacctggt cgtgcgcatc cggcaggaag
     4141 gcgtggtcca gcaggctggc cggggcggcg gcgagcgcgg cgtggagcga ctcctcgacc
     4201 gcggcggtcg agccgcccag gatgacgagg tagcgcccgc ggctgacggt gccggatttg
     4261 agcatggcaa tgggcgcctt tttcagcacg gcgtcggtgc aatagaggcc gtcggccagg
     4321 ctgctgaact cgatgagggc gagggcggga tgcttcttca tcagacgatc cggaaggcgc
     4381 ccaccagcgc gcagcggcgc cagcgcgaga aactgcgcgg gctggtcatg ccttcgccgg
     4441 agggactggc gatggtgaac gaggtatggc cttcgccctg ccagcccagc ccggccaggg
     4501 cggggccgtt cttgacgaag atgctggcgt cgcactcgcg cgccatgcgg ctgaggtgcg
     4561 agaggttgtc ggagtgcatg acgaaggtat ggcggcgcgg atgctccagg gcgatggcca
     4621 ggtcaatccc ctcgtcggcg tcgcgcacgc gcgtgagggg caggatcggc atcatctgct
     4681 ccgtccacag cagcgggtga tcgggcccca cctcgacgat gcccagccgc accgtgtcgg
     4741 cgcacgcaat gccggccttc cgcaggatca cgctggcgtc ctggcccacc agcgcgcgct
     4801 ccatctcggc ttcggcgcgc gggccccgcg tcttccggaa gatgaccttt tccagtgccg
     4861 gcaactggtc tttccagacc agggccgcgc cgtggtaaat cagtccctgg atgagtttgt
     4921 cggccaccgg ggcggccacc aggcacgcct tttcgtcggc gcagatgatg ttgttgtcaa
     4981 acgaggcgcc gaagacgatg tcgcgcgccg cctggtcgag gttggcggtt tcatccacca
     5041 cgaccggcgg gttgcccggc ccggcgcaga tggcgcgctt gcccgattcc atcgccgctt
     5101 tgaccacgcc gccgccgccg gtcaccacca ccagccggat gccggggtgc cgcatcaggg
     5161 cttgcgcgct ctcgagggtc ggagtggcca ggcaggtgat gagatggcgc ggcccgcccg
     5221 cggcgaggat ggccttgttg agcagcgcga cggtgtggca ggagacgcgc cgcgcattgg
     5281 ggtggacgtt gaacacaacg gcgttgcccg ccgcaatcat ccccagggcg ttgttgatga
     5341 tggtggaggt ggggttggtg gtcggggtga tggcgccaat gacgccgtag ggggccggct
     5401 cggtgagggt gaggccgtgg tcgccggtga cggtctgcgg ctgaagatcc tcggtgcccg
     5461 gggtcttttc ggtgacgagc tggttcttga gcaccttgtc ttcgtagcgg cccagcccgg
     5521 tttcctcgtg cgcctcgcgg gccagcgcgc ggccatgctc gcgcatcgcg tcgcgcatgg
     5581 cggcgagcag aacattgcgc ttttgcaggc ccaggccgct caactggata aacgcatccc
     5641 gggccgcgcg caccgccgcg tcgagatggt caaagacgcc cagctccccc gccacggaag
     5701 ggcgggcggg gcggtccggc cggggcgggc cgtcgccggc gagtccggcg acgatctgcc
     5761 gggcaatggc ctcgatttcc tggtcgctga ggccgcccac gggcgctagt cctttcggta
     5821 cttgtccacc ccgccgatct cccagctatc cacaatggcc atgatgacgg cgtcgcaggg
     5881 gcggttctgc gtcgtcacgg tctggcgcgc cgaactgccg gtcgcaatca aaaccatctc
     5941 gccggggccg gcgcccacgg catccgccgc cacgacatgg ccgccggttt cccggccttc
     6001 gggatccacc gcccggacga ccatgaactt caggccgtcc agggtggctt ctttgcgggt
     6061 ggcaacgacc gtgccgacta cttttccaag cgtcatagtt tcagtccggg ccccgcccgg
     6121 gcggcggggc ggcgcttccg gccggcgcgg gccgcggccc ggtccggccc ggaaggcggg
     6181 ttactttttg atcgccttgg cgccgcggcc cagcggcagc acctcgtcca cattcgcgtg
     6241 cgggcgcggg atgacgtgca cggccaccat ttcgccaacg cggctggcgg cgaggcgccc
     6301 ggcgtcggtg gcggccttga cggcggccac atcgccgcgg atgacggcgg tgacgtagcc
     6361 cccgccggtt ttctcgaagc tgacgaggtc aaccttggcg gctttgacca ttgcgtccgc
     6421 gccttccacc atggcggcga aggaccgtgt ttccagcaag cctaatgctt ctgccatata
     6481 tgagtctcct tatgttgtct ggttatgagt gagggtttcg caaagtgaat caggcgccgc
     6541 cgccgcccgt catcggccgg ccggcaaggc tgtggatggc ggcctcggcg gcctgggccg
     6601 ccacgtcaat gtctttctcc tcgccgccca ggtaaagccg gccgaagctg ccgaaggggc
     6661 gaatctccag gatgttgatc tcggcggttt tctcggcctc gttggcggca aaggcggcat
     6721 aggccgcggg ggcgacctcc atcgtgaaga ggctctgccc cggcaggatc atgttgccgt
     6781 gccgggtgcg gttgatgagc atcgtgtggt gatcatctat ctggcggatg gtgctgcggc
     6841 tgtagatggc cggtttatag cggtcctcct ccttcagccc gagcgccgcc aggatggccg
     6901 cgccggcgga gcggacgtcc gcctgcgact cggaatggat ttccagcagg ccgtagaggc
     6961 gctcgatgat ctgcatgccg ggcgtgacct tcgtgcactt gagcgccacg tcggtgacga
     7021 agttgatttc catgccgggg gaaatctcga ccagcagcga ggcctgcccg gcaatgggca
     7081 ggaacccctg cgagacggtc gccaaaaacg acgccacctg cggttggagg cggtccagaa
     7141 aaatgtaaga acgaaggtcg gccataatga gaggttgtcc tttcctgcct agtaggtttc
     7201 tcccccgccc gggacggctt cgccggcggc ctcctgcagg attttgaggc cgctgctggt
     7261 gccgatgcgg gtggcgccgg cctgcaccat gcggcgcaaa tccgccagcg tgcggattcc
     7321 cccggaggct ttcaccccca gccctttggc gcgcacgatc cggctcatca acgccacgtc
     7381 ctccaccgtc gccccgccgg agctgaaccc ggtcgaggtc ttcacaaagt ccgcccgggc
     7441 ctggatggcc agctcgcagg cgcgcacctt ttccgggtgg ttcagcagcg ccgtttccag
     7501 gatcactttg caccgggcgc tgccgtcccg gcaggcttcc accacggcgc ggatgtcgcg
     7561 gagcaccagc gcgtcgtcgc cgctcttgag cgcgccgacg ttgaggacca tgtcaatttc
     7621 ccgggcgccc tgccggatgg cggcgcgggc ttccatcgcc ttggtctccg gcggctgcgc
     7681 gcccagcgga aagccaacca cgcagcaaac ctttacaccc gagccgtcca ggagctggcg
     7741 ggcgagcgcg acccaggtgg ggttgacgca aaccgagtag aactggtatt ggcgcgcctc
     7801 gcggcagagc tgctcgatgt cggcccgggt ggtatccggc cgcaggatgg tgtggtcaat
     7861 cagcgccggg atattcagcg gcggcggggc ggggggcgcg gcggcgggct cgccccgggc
     7921 ggacagcagt tcccggacgc gctgggcgag cttttccacg tccgccgccg cgggcgccgg
     7981 gcggggcgcg ccgactccgg ccagcagcgc cgcctggtgc ggccccactt ccagcgcgcc
     8041 ggccgggacg gcattgatga gctcgacccg ccggcggtgc cgctcttcca cgcattccgc
     8101 cgacaggaac gcttcgacga tggcctgggc ctgctcgaaa tccgtctggc ccgcccccag
     8161 cgtcagcacg ttggcgtcgt tatgctcgcg gctgtttcgg gcgagggcga cgttataaca
     8221 ggcggccgcc cgcacgccgg gcaccttgtt ggcggtcatc gccgagccga tgcccgcgcc
     8281 gtcaatcata atgccgcggt cgcattggcc ggccgccacc agccgcgcca cggtgtaggc
     8341 gatgcgcggg tagtccacgg cgtccttgct gtgcgttccg caatcctggg tttcgtagcc
     8401 gcggtcgcgc agccaggcca ggagccgggc tttcagttca aacccgccgt gatccgcgcc
     8461 aatagctatt ttccggttca tcgcactggc tgtggggatg gggcgccctt cctcgccgcg
     8521 gcgaggcggg ccgaaaatgc actgttcggt tcacccacgc gcgccactct aacaggtggc
     8581 aaaggggtgt caagggcacg ccccgccgcg gccgccgggg aaccgcccgg gcgctcccgc
     8641 cgcggggtcc ggacgcggcg cccgggcaga ggcttccggc gattcccgcg cgcgccgcgg
     8701 caaacgccgc ctcaggggaa attgaccttc acctcaaaga acccgacgct ggccttcgta
     8761 ttggtattcg tccagtaagt ccggttcgta gagtaaccaa agccaaacgg cgaggtctgc
     8821 tgcatccagg cgccggtgag ggaggacttg gcgcgaatgc gataaggctg gcggctgccg
     8881 gcgttgggcg ggctgggcca ggaaaggaca acctggccat tcggcaggcg ccagatcgaa
     8941 tcaatccgcg tctgggccag cagcagcgtg ttccccgcat taagccgtcc ccagccgtaa
     9001 tacgggtcaa aacccggcgt gtccgccgcg tcgccaacct ggtcgtcggc cccggcgcgc
     9061 agcagggcgc gcgcctcgct ctgcgtcagg cccggccgca ccgccgccag cagcgagcag
     9121 acgcccgcca cctggggcgc ggcaaacgaa gtgcccgacc aggtggccag ggtcccgctg
     9181 cggctcaccg aggtgatgcc cacccccggc gcgaccaggt ctatttccag cccatagttg
     9241 ctgaagccgg cccgcacatc gttccgatcc gtggcgccga ccgtgatgca gatcggcagg
     9301 ttgcccggaa aacggatggc attcgtcccg tcattgtggg cgatggtgac aaagatgact
     9361 ccctgcgcga tggccctggc gatggcgttc gacagggtta tgctgctgct ggcgccgccg
     9421 gcggagaggt tgatcacctt cgcgccctgc gccaccgcgt aatcaattcc ctgggcccaa
     9481 tcggagtaat aaccactgtt attccggtcg agcaccttga tgggcatcac ccggcaatgc
     9541 caatccaccc ccgcccccag cgccccgttg ttggcgctgg cgcacagaat ccccgccgcc
     9601 atggttccgt ggccgtgatc atccgccgtg tcggtattgc aagccgggtt ggtgaagcga
     9661 taaccgggaa ccacccggcc ggcaaactcg ggttgatcgc cgtttaaccc cgtgtccagc
     9721 atggccacaa tcactgcggg cgagccttgc gtaatgttcc aggcctgcca ggtgtggata
     9781 ttggccgttg gtttcacgct gttggtatga tgccactgcc gcagaatatc cgggtcgctc
     9841 gggatgaccc ctcccgtgcc gatgccatcc ggctccgcat actcgaccca gccatgccgg
     9901 ctcaactgct gcagcgcgtt ggtgacccgc atccccggtg gcaaatgcag ccggagaaag
     9961 cggctcacat ccacgggcgc ctccgtcccg gggcccgcgc cgcggcgcga acgcgaacgc
    10021 aagagccgtc tcacgggcgg ttcctccagc cgcgcctccg acggcagccc aagcccggcc
    10081 gccagtgtgg tcagcgcctc cgtctcagat tgagtggaca ggagtgggag tttctccgct
    10141 ttgagtttca ccagcagatg cgtctccgcg tattcgccgc gcggggcatg ggttccggag
    10201 accggctgca agcccgtgcc agccagaaga acagccacca gccagaactg ccggcgaaag
    10261 aaagcccggt cactcatcta ctgcgaatta cggatagcct atcaggacac atacgtcgcg
    10321 acaagtcctg acagcagtaa aaatgccatc ccctatgccc tggcctcacc catattcatt
    10381 tccagggctt tctccaacat cagcttcatc acgtgaaagc ggtcgaagac gatcagcgtc
    10441 caggggcgga aggtgtttat cgccgtgggt cagccccgga acgcctggaa aggccaaagc
    10501 cgggttcaca acgccatcgt tccgccaccg ccaaccttga aatctcagca aaccgctgaa
    10561 acggcagcgg ttaagaaatc gaagcccgcc accgccccag cttccaccac cgcccgcgag
    10621 gccgttcctt ccccatcctt tcaagccatg tacaagggga gtacatcggg agcgcggcag
    10681 gagaacacgg aaaacaagca tgagctccct ttgagcctcc gttgtacacc cgttgtacac
    10741 ggcctgttgg gagcgtcagg ctggccgggg cgggtgggac gggtttgggt gcgagcctgg
    10801 gccggaatgg gggaatgggc gggatttgcc gctctttggt gctttgctac cgcgcccgga
    10861 cgtggtatgt ttcccccgat aacagatgga aagcgagcga atgcggcaga cgcgctgagt
    10921 tgaaagcact ggtcgagaac aggaaccacc ggacggaaag agtattcctc atcggggtgg
    10981 agctgaaatc gcggcgaggg gccgaggtgg acgattcgct caaggagctg gccgagctgg
    11041 ccgccaccgc cgggggcagc gtggtggggg atggtttgca aaaggtggcg gctccgtgcg
    11101 cggcgacgta tatcggcaag ggcaaggcgg aggagttcgc ccgctactgc cagcaggcgg
    11161 aggtggatac cgttattttc gacgatgagc tgaccccggc gcaaagccgc aatttggagc
    11221 gggtctttgg ctgcaagatt ctggatcgca cggcgctcat cctggatatc tttgccgcgc
    11281 gcgcgcgcac gcgcgagggc aagctccaga tcgagctggc gcaactgcgg catttgctgc
    11341 cgcggctgac gcggttttgg ggtcacttgt cgcggcagaa gggcggcatc ggcatgcgcg
    11401 gcggcgaggg cgagacgcag ttggagaccg accgccggcg ggtgcaggac cgcatcgccc
    11461 ggctggcgcg cgagctggaa gtggtgcgcc gccagcgttc gctccagcgc aaccagcgcc
    11521 agcgcaacca ttgggcgctg gcctcgattg tgggctatac caacgcgggc aagtccaccc
    11581 tgctcaacgc cctgaccggc gcgacggtgc tgacggagga caagttgttc gccacgctgg
    11641 atcccaccac gcggcggctg cggctgccga cgaatcagaa cgtgctggtg acggatacgg
    11701 tcgggttcat tcgcaagctg ccgcacgggc tggtggcggc cttcaaggcg acgctcgagg
    11761 aggtggtcca ggcggacttg ctgctgcatg tggtggatgc cagccacccg gacgccgagg
    11821 cgcagatcca ggcggtgaac gcggtgctgc gggaaatcgg cgccgacggc aagccgacgg
    11881 tgatggcgct gaacaagctg gaccagctcg cggagcgccc ggaggtgctg gccggtttcc
    11941 aggagcggta tccgcacgcg gcggccattt cggcggtgac gggcgagggg attcccgccc
    12001 tgctggcgga gttgagcagc cagttgcgcc cgctccgcga gttcatggag ctttccgtgc
    12061 cgcacgagca ggcgggcgtc attgcgcggc tgcatgaggt ggggcaggtg gtcgagcggg
    12121 attacaacgg ccagaccgcc cgctttaaag cccgcatccc gccgcacctg catgatgagt
    12181 ttgcgccatt cctcgtccgc gaattgcaga cggcttgagc gggggcgcgg cgggccgggc
    12241 cggggatgag cggcggcgcc cggcggcgcg cgccggcggt ttgggcttgg tatgggcggg
    12301 cggcttttgg ttaaatgaag cccagatgaa atcccgacgg gcccatatcc gccgcgccac
    12361 gaacgagacg cggattgcca tcgagttgaa tattgacgga accggacggt ccgtcatcca
    12421 caccggcata ccgtttttcg accacatgct gacgttgttc gcccggcatg cggtggtgga
    12481 cttgaagctg cgcgggcagg gggacctgga ggtggacgcg catcatacgg tggaggattg
    12541 cggcctggcg ctggggcagg cgtttgtcct ggccctgggg gacaagaagg gcattcgccg
    12601 ctacgggacc gggttcgatc cgcgcaatcc gtttaccggc gaggcgtata tcccgatgga
    12661 cgagtgcctg gcgcggtgcg tgattgattt tggcgggcgg ccctgcctgg tctggcgggg
    12721 gatggcggcc ctggcctaca agcgcgtcac ccgggcggag cgggagcagg acatgtccag
    12781 cgcgttccgg ttcggcctgg cgcgcgagtt tttccagggc ttcaccaacg aagcccgctg
    12841 caacctgcac ctggagctgc tttacggcga tgagccgcac cacatcgtcg aggccctgtt
    12901 caaggccttc gccaaggcgg tggacgcggc ctgccggcgg gacccgcgga tggcggggca
    12961 gttgcccacc accaagggaa aattgtagcg cgggttgaat acggggcgcc tcccgggcgt
    13021 cgaatgcatg actgtcaaag actattcctc cgccgaagag cgggcgctgg tgcggatggc
    13081 gcagcagggc gacctggcgg cgttcgagga attggtggcg cggcaccgcg acaagattta
    13141 cgcgcgcgcc tacagcatga tgcgccatga acaggaagcg ctggatttgg cgcaagaagc
    13201 gtgggtcaag ggctggcagc ggttgggcca gttccagggc gatgcggggt ttggcacctg
    13261 gctgacccgg atcgtcatca acctttgcct ggatgcgctg cggcggcaaa agcggcagcg
    13321 ggtcgagtcg ctcgaggcgc tcgcggaaga gtcgggcggg gttgagcggc agatgccggt
    13381 ggtagtgccc aatcccaccg ccgggctgga gcgcgccgag ctgcgccagc gaattgacca
    13441 ggcgctgggc cagttgtcgc acgagcatcg cacggtgctg atattgaatg aatttgaaga
    13501 aatggactat aaagagatcg cgcggacgat gggctgctcg atcggcacgg tcatgtcgcg
    13561 gctgttttac gcacgccgaa aactggccgc gctgctggcg gatttgaaga aaccggatac
    13621 aacctgatgg acctggcggc acaactgaaa ttgcaagcct ggctggacgg cgagctgccc
    13681 gaagccgagg cgcgcgcggt ggcggattgg gtggcccggg agccggaagc gtcggcgctg
    13741 gccgaggaat tgcgccaaac ccgccgcgcc ctggccggct tcgaggcgga cctgcggctg
    13801 ccggaatcgc gcgagttttt ctggtcgaag attgagcggg agattgcccg gcgcgcggcg
    13861 ccggcgccgg cgtccgctcc gtggggggcc gcgatcctgg ccgggcttcg ccggttcctg
    13921 attcccgcca ccgcggcggc tttgctggtt ctggttggcg cggtgctgat ccgcccgggc
    13981 ggggcgccga cgcggggggg cggggcggag gtcgagatgg cgctggccga cgccggggcg
    14041 ttcacctact gcgattattc cgccggcacc acgctggtgt ggttttcgta tcccgccgac
    14101 aactagattg cgccgcgcga ggagatggct acaattccgc agttaatgcg agcaagaccg
    14161 atgatcatgg ctccctgggt ttgggtttcg ctggcgctgg cgctgctggc cagcgccggg
    14221 tttggcgcgc cgcccgccga catgaaactg caggcctatc ttctctgggg caccaatgac
    14281 cgcaatccgc cggcggacag gaattaccag ccggtcaatc ccgacatccg gcaaaagctc
    14341 aaatccctgc cgctcaagtg ggcgcattgg tatgaagtca agcgggtgga cttccaggtg
    14401 gcgccgggcg cgtcccggga ggtcacgctg agcgacaaat gccaggtcaa gctccgtcag
    14461 cttgcctccg ccgaggtgga ggttctgctc atcggccagg gcaaagaagt gatgaagcgg
    14521 cggcaagccc ttccgaaagg agacttgctg gtcctgggcg gcaattcccc caacgccacc
    14581 gcctggctgg tggtcttaaa gcgcaacgag tgacccggga gcgcttgccc cgctccgccc
    14641 ggccgccggc cggggagcgc aagcccctgc gcgcccgggg tcgcgcgcat cgtccggctc
    14701 aatgattggg cagccggaaa ccgcccgggc cccgcggccg gcgaagtgaa cgttccggcg
    14761 gcaacggagt tgcgcccgga atgcgggaag ctaaagccgc cgggacagcc attttaatat
    14821 gcacgactgg atcaactatc gaagcgaacg aaagcgcagt ttgtggaaag ccctggtgtt
    14881 ccttgccggg gcggcggtca tcggcctggc gcccagcgcc ctggcccgga agccgtccta
    14941 tcaactttgg gcggtcagcc cgctgaccaa ggttctgcaa gatcaagccc ggcccacgaa
    15001 cgcgtgggac cgcatcgccc tgtatgccgc caaaggggaa agcgaggccg ggcaggtcgt
    15061 gatttcggcc ggcagcgagc cgctcaagga cgtgcgcgtc agcctggagc cgctcaccgg
    15121 ccccggcgga gccatcaccc aaatagaact gagcaaggtg gtttatatca acctgcccaa
    15181 cctcaagaag atcgtcccgg atgcgctgcc gccggcgacg ccctttgagg tggcggcgca
    15241 ctcgaatcag ccgatctgga tcaccctcat catcccccgg gacacgcggc cgggggttta
    15301 ttccggcaaa ctggtggtca aacccgccaa cgcggcgaag cagcgcatcc cgatccgcct
    15361 caaagtgtgg aacttcaccc tgccggaaac gccgagccac aaaacggcgt ttggcatcag
    15421 ccggaatctg ctgccgcggc agcacggtgt tttgaagggc agcgaagcgg cggagcaact
    15481 ctacgccagg tattacaact tcctgctcga tcgctggata tccgcgtact ggctgccccc
    15541 cgcggtcacc gcgccggagg cggagcgcta tatccgggat ccgcgcgtga cggggttttt
    15601 gattccttac aacgatgatg tggaggagat gcgcaaatcg gtccaatcgc tgcgccgccg
    15661 cggcctgctg gataaggcct atttttatcc cgtggatgaa ccctacaccg agccgcatta
    15721 caagcgcctg gtcgaggtct gcacgaaaat ccattccatt gatcccaagc tgcgcatcac
    15781 gattccctac tatcgcgacc ccagtttcga taccggcggc aagacgctgc acgaactggc
    15841 cgacggcctc atcaacatct ggtgccccaa catctggtat ttcaatgaga acaaggagcg
    15901 cctgttcgcg aagctggcaa agggcgagga gttctggtgg tatgtgtgct gcgatccggc
    15961 cccgccgtat ccgaactact ttttgaacct ggacggcccc agccaccgtg tcctgccctg
    16021 gatgggctgg aagtatcgct gccaggggct gctttactgg aatacgacct ggtgggacga
    16081 gggtaagaat ggaaccgagg atccctggac ggatatggcg accgtcaagg ggattaacaa
    16141 gaacatgtat ggcgacggtt cgctgctcta ccccggcaaa aaggtcgggg tggacggccc
    16201 ggtcagctcc atccgcctcg aactgctgcg cgaagggctg caggactacg agtatctcgt
    16261 gaagctggag cagaaaatcg gccgggccgc caccgagaag ttcgtctccc agctcgtgac
    16321 caaccccatg gacttcaacc ataacgtgga tgactgggtc cgggtgcgcg tggccatcgg
    16381 cgaggagctg ggcaaatagg ctgatccgcc ggcgtgtttt tcgttcgctt tggccgttga
    16441 cacgccgggc gcaaatgcct aagctcgccc caacaactaa atcaagcacc acacatgttg
    16501 gtccgaatct gcttaattgt ggctgtcctc gccggcttgg cggtgggcgt cctgaacttc
    16561 ctccaggtca aagagaagat caccgtcctg caggctgacc tcgagcggga gaccgcgctc
    16621 catcacgagt tcgagggcaa ataccagacc acccggaagg agctggacgt ctgctccaaa
    16681 gagctgaaga acaccaagga cgagctggcc acgacggtgg cggcccggga caaggccgag
    16741 gccgacctgc aggcgcaaac caagcgcgcc gataaattaa ccgaagacct gaacaagact
    16801 cgccaggagc gcaacgacgc ccaggcggag ctggcggctt acaaggtgac cggggtcacg
    16861 ccccagcaga ttctggccat gaacaagcag ttcaagtccc tccaggacaa cctgcaggtg
    16921 gcggaagccg agaacaagct gctgaaccag aagatcaaga agctcgatac ggagctctcc
    16981 gtttaccgcg accccaactt cttcgtgtcg ctgccggcgg cgctgaaggg caaagtgctg
    17041 gtcgcggacc cgaaatggaa cttcgtggtg ctcaatatcg gcgaagacca gggcgtcctg
    17101 gaacgcggcg aactgctggt caaccgcaac ggccagctcg tggccaaggt ggtcgtgcgc
    17161 agcgtgcaaa aggaccgctg catcgccaac gtcctgcccg gctgggaact gggacaggtg
    17221 atggaggggg acgtcgtcat ccccgcccac ccctcgtcgt gaaagccgcg gttttgcgtt
    17281 tgcgctgggg cctgctgctg ctggctgtcc ttgcggcggt cgtgggcggc ggctgcgcca
    17341 ccacggaatc ggaaaacgcc gccgagacgc cgtggaaccg cccgcaaggc tgggaaaccg
    17401 gcctgcccgg cggcatgtac aatccttacc cccgataacc ccgtgccggc gccccgccgc
    17461 cgggagttcg ctacgcgggc gaaagcggcg gccgttcgat ccagcgcgtg ggcgtgagca
    17521 ggcaatccag gcgcgcgtcg tgcggctcgc acgggatttc gttccgcagt tgaagatcaa
    17581 accccacccc gcaggtcgtt ccgggccatt cctttagcag gcggtcgtag tatcccttgc
    17641 cccggcccaa ccgcgcgccg cgcacgtcaa aggcaatccc gggaaccagg atcaggtcca
    17701 gccggcgcgc cgccagccgg gcgcagcggg ggcccggctc gcgaatgcca aagcggccca
    17761 tgatcaggtc cgccgcggca tcctgaatcc cgcagacttc gtaggtttgg gttgcggcgg
    17821 cgaagcgggg cagggcgacg tttttgccgg cggccagcgc ctccggcagc agcggccaca
    17881 cgtccagttc ctgtggcagg ggcgcgaaga aaagcaccca ctgggccgct tgccactgtg
    17941 gctgcgccgc caggcgggcg cgcgcctggg ccgcccccgc cgcgcgttct tccgggccaa
    18001 gccgggcaag cgcggcgcgc acttgccgcc gcagcgcctg tttcgcctcc tggattgcct
    18061 gcgcggagtt caccgccggg tcagcccttt gcctccgggt cccgggcttg cgcctgggcc
    18121 tgcagcgccc gccagtgggc cagccggtct ttgatctttt tttccactcc ctgctcggtc
    18181 ggttcgtagt agcgtttatg cgccccgaga tagtcctgca ccacgaaatg ccctggatga
    18241 tcatgggcgt actggtagcc ctccccgcgg cccagccgct gggcgccccg gtagccggcc
    18301 tcgcgcaagt gctcgggaac cgccagcgtg cggccggtgg cgacgtcttc cagcgccgcg
    18361 tcaatcgcca ggagggcgcg gttgctctta tgggccgtgg cgatgtagat ggccgcctcg
    18421 gccagcggaa tccgcgcctc cggccagccg atgaactcgg ccgcctgaaa cgcggcgttg
    18481 gccagcacca gcgccatcgg gtccgccagc cccacatctt ccgcggcatg gatcacgatc
    18541 cgccgcgcaa taaagcgcgg gtcctcgccg gcgtgaatca tcttcgccag ccagtacagc
    18601 gtggcgtcgg ggtcgctccc gcgcatggac ttgatgaacg ccgaaattgt gtcgtagtgc
    18661 gcatcctcgt catcatagac caccgccttt ttctgaatgc tttgttccgc caccgcgagg
    18721 tcgaggcgga tgacgccgtc cgcctccggc gccgtggtga ggacggcgat ttctagcgcg
    18781 ttcaacgcct tgcgcgcgtc cccgtcggac agcttcgcca gatggcgcag cgccgcttcc
    18841 tccgcctgga ctttaaggta gcccagcccg cgttccgggt ccgccagggc ccgctggagc
    18901 agctccagca acgccgcttc ggccagcggc cgcagctcaa agatctggga gcgggagacc
    18961 agcggggagt tgacgaaaaa gaaggggtta tgcgtcgtgg cgccgatcaa ccgcacgacg
    19021 ccggtctcga catccggcag gagcacatcc tgttgcgcct tgttgaagcg atggatttca
    19081 tcaatgaaca ggatcgtgga ctggtgtttg ttggcgagcc ggttggcggc ggcggccagc
    19141 acgcggcgca tatcggcgac gttggattca accccgctca accgctcgaa cttattgcgg
    19201 gtctggcggg cgataatctg cgccaaagac gtcttgccgg tgcccggcgg gccgtagaag
    19261 atcagcgatt ggatgcgatc cgcctcaatg gcgcggcgca gcagttgccc cggccccagg
    19321 atgtgcgcct ggccgacgaa ctcgcccagg tcgcgggggc gcatccgggc cgccagcggt
    19381 tgatgccgtt cggcgccgga ttccggcgcg cccgcgtccg ccggggccgc cccgggcgcg
    19441 atggaaaaca aatcatcccg cgacatgccg tcaccatatc agcccgcgtc cgccttgccc
    19501 aaaccaaaaa aggcccggcc ccggcttcgg cacgcctcaa ggttgtcccg aattgaggca
    19561 ctcattgtcc cgggaaagag cggggggagg tgtttttcgg gacgggataa gagtttgccg
    19621 cttcagaggt tttgcatagg ggccgggttc gcgcagccac atcccggggg cagaacggtt
    19681 tagttgggga gttcaaccgt ccgccctcgt ttcatcgtat cctgggcggg cttgatcgcc
    19741 caagcggcag cctcgacgaa cccttggttt agcgaatccg agagtatggc tgctgaatcc
    19801 cgagcctttc gctggtaaaa tcaaggattt aggcataaag aggggcaaaa taaggccaaa
    19861 acctgaaccc tgccgccttc ggcgcatccg aaacatccct tggatttacc ctgccttcgg
    19921 gccgtgtaca aggggtgtac aagggatgct caccgggagt ccataggatt ttag
//
LOCUS       DAOPQG010000068        19365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3449_length_19365_cov_14.967996, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000068 DAOPQG010000000
VERSION     DAOPQG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 19365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19365
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3449_length_19365_cov_14.967996"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKI07_08930"
     CDS             <1..190
                     /locus_tag="PKI07_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51214.1"
                     /translation="KAPQPNPHHLGWTGQGASFRRNNPPSLTSLDYTDSSTSSGTSPA
                     FRGTAIIPPGLPLPPRGG"
     gene            284..1273
                     /locus_tag="PKI07_08935"
     CDS             284..1273
                     /locus_tag="PKI07_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOC51215.1"
                     /translation="MKTLTTFLVLATLALCAFGREAQAAAQVPAPARTYRNPLLPERE
                     IADPFVLRFNGQYYLYSTTDSRGYEVFVSENLVDWKNKGRVFRDPRGGNWAPEVFHNQ
                     RGDGKFYLYYTDNIGGPRRGPLEKQIGVAVADSPLGPFIDQAVLATHSIDAHLFQDDD
                     GRLFLYYVDLNEGFKIMVQPMADPIRKVNEPKVVIRPTVEWERRSGEVTEGPFMLKHD
                     GIYYLMYSGSGADTPNYGIGYATSKSPTGPFVKYAGNPIAQRGGKVSGPGHHCVVEGP
                     DGKLWMLYHQKWDTEVNWRRFLALDPLWFDSQGVLHVKVTRDTDQPAPGIPAK"
     gene            complement(1355..2257)
                     /locus_tag="PKI07_08940"
     CDS             complement(1355..2257)
                     /locus_tag="PKI07_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC51216.1"
                     /translation="MKYMGIGDEAANNIDGQIQATQELGWKLLEPRGVEVPGFAKANF
                     HDIPDEAFDLAVRKLEAAGINAYCFGSTIMNWAKKLSDPFDITLAEVKRAIPRMQRAR
                     AKYVRIMSFKPEDDACVIPAEVFRRVKEVTDRFLDAGLQPVHENCMNYGGMSWQHALE
                     LLDKCPGLKWVFDTANPIFNPDRSKPKPWPKQDPWEFWTHVRDHVAHIHVKDATWNPA
                     KDDADYNWPGEGRARVRDILKDALSRGYNGGLSIEPHMVVVFHDAQAKAANEAAMRQN
                     YVEYGRRLEKIVAEARNELKQPAA"
     gene            complement(2794..3948)
                     /locus_tag="PKI07_08945"
     CDS             complement(2794..3948)
                     /locus_tag="PKI07_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51217.1"
                     /translation="MKHVRLGLIGLGNIGQHHFNYLTAGKISRAELVAVSDTASAKLE
                     KYKPLKTFIGGEELIRSGLVDAVIIATPHYQHTTLGIAALKQGLHVMVEKPISAHKAD
                     AERLIAVHKTKPRLVFAAMFQLRTEPRYLKIRKLIQDNELGEIVRMSWVMTDWFRTEA
                     YYASGGWRATWKGEGGGVLLNQCLHNLDTLQWLLGMPARVRGFCQLGRFHQIEVEDNV
                     SAYLEYPNGATGTFVSSTGESPGTNRFEIVGTRGKLLLEADKLTLERNEVDMIEFSRT
                     ARLGFAKPEVWHVEIPFGPSPNGHAVLMQNFINAILDGEPLITPGPEGIHSVELANAI
                     LFSSLLGKTLELPMDSRAYEKKLNQLIAASKVKKKVVAIANEDFTKSFRR"
     gene            complement(4051..4770)
                     /locus_tag="PKI07_08950"
     CDS             complement(4051..4770)
                     /locus_tag="PKI07_08950"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HOC51218.1"
                     /translation="MIGTALNVAGILLGGAIGLARRKPFSPARESFFKVALGVFAIFY
                     GLRLTWTSLNGSLWQILRQLLIVVLALMLGKLTGRLLRLQKMSNRVGKKARESIAAAR
                     PAAPGRLGEGFKTCAALYCVAPLAILGSVQDGLSGYYYPLAVKAVMDGLATMGFTLVF
                     GWGVLLVALPVLAFQGTITLLCLHGLKPFLEAHSLVDPVNAAGGLLVFCVGMVILDLK
                     KIELADYLPSLIFAPILSWLM"
     gene            5168..6382
                     /locus_tag="PKI07_08955"
     CDS             5168..6382
                     /locus_tag="PKI07_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOC51219.1"
                     /translation="MPDFFQTGAIATLHRLGRPDVARLEHELERFAAESPITLVLPCH
                     IKELGTRALARIVRELKNVSYLKQIVVGIDGATRAADFQRACKFFAQLPQRPMLLWND
                     GPRLSALFQKLEEAELSAGPGGKGRNIWICFGYVLASEQARVVVVHDCDILTYSRELL
                     ARLTYPVAHPSLGFDFCKGYYARVTHQLNGRVMRLLVTPLLRALKSIIGQHPYLVYMD
                     TFRYPLAGEFSIHTDLVRRVRIPYDWAVEVGTLAEVFRNAAPRAICQSELCENYDHKH
                     QELSPRDTAKGLNKMAVDITKSFFRRMAAEGIKLDVGLFDTLLSAYLRQAEDSLRFYG
                     ADAAVNGLSFPRHEEEKAVATFVRSIQIAEKAFLEDPLSTPLIPNWNRVQSALPRFFD
                     GLNAAVRLDNTH"
     gene            6467..8191
                     /locus_tag="PKI07_08960"
     CDS             6467..8191
                     /locus_tag="PKI07_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HOC51220.1"
                     /translation="MTSQFRQYLDFLYGREGAAASMRQLSVLLKSWPRQTLGRFRRTV
                     KPLAEHDILLITYADQLREPGEAPLRTLARFCKSHLQGLISGVHILPFYPASSDDGFA
                     VKDYFAVDPAFGSWNDVARLGQSFDLMFDAVVNHLSAQGEWFRRFQRDDPQFRDFFIT
                     VKGQPDLSQVVRPRTLPLLTEFPTARGRRRLWTTFSADQVDLDYRNPRVLLAVLEVLL
                     FYAARGARFIRLDAIAFLWKEIGTTCLHLPQTHAIVQLMRAVLDKAAPHVRLITETNV
                     PHAENVSYFGNGANEAQLVYNFALPPLVLHSFATGNAVKLTRWAQSLTLPSDRVAFFN
                     FLASHDGIGLNPVQGILTKAEIEALASRALAHGGFLSFKELPDHTQVPYELNINYLDA
                     LSNPRRGEPAELAARKFLTAHAILLSLQGVPGIYFHSLLGSRGDRAGAETSGLKRRIN
                     REKLARAQLESELAEAGSLRACVFGGLNKLLRIRRQHRAFHPQAAQRVLAGDRRLFAV
                     YRASLDGRQRLLCLHNVTSQTVSASRLVQAAECAPFWTELISGDRYQPANVRLKGYQT
                     LWLRAEDK"
     gene            complement(8201..9490)
                     /locus_tag="PKI07_08965"
     CDS             complement(8201..9490)
                     /locus_tag="PKI07_08965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC51221.1"
                     /translation="MSFCILTTGAPKRIGFISTRFHGTDGVTLETRKWAQILTGMGHS
                     CFWMAGLLDAPPEASHVAPLAFFGHPEVAEVQSRLFGVAFRSRAVTNQIQSIKERLKD
                     ELYRFIEKFQIEILLPENILAIPMHVPLGLAMTEVTAETTLPVIAHHHDFAWERERFT
                     LNAVSDYLQAAFPPLFYKMEHVVINSMQQKELARRLGIPSQIIPNVLDFETPPPGVDD
                     YSRDLRREIGLEESDWFILQPTRVVQRKGIEHAIELVRRLNNSRAKLVISHPAGDEGN
                     AYMAMLKDRIADAGIDVRFIFDRVGETRGTTPEGKKIYTLADVYPHADLITYPSHYEG
                     FGNAFLEAVYFSKPVVVNTYAVYARDIDPLGFKTIEMPQLVTREVVEQARAVLEDQAL
                     AAEWARTNYALGVKYFSYSVARRKLAARISNLFGEGV"
     gene            complement(9603..10343)
                     /locus_tag="PKI07_08970"
     CDS             complement(9603..10343)
                     /locus_tag="PKI07_08970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC51222.1"
                     /translation="MELADVNVRAVIFDVYGTLLEIGPPPVDADARWQQLFQEMLHAP
                     PLLSRLDFYVASNKLVAKRHEASRARGIAWPEVQWPAVVAEVLPPLRRLPRAKLDEFL
                     YRQIQIGHTPLLSDATVAALRKLKASGCLFGIASNAQAYTLRELEEALVRHGLGMDLF
                     ERDLCFWSFEHGFSKPDPHVFQILSTRLEARGISRAEALMVGDRLDNDCEPARAFGWQ
                     TWLLKPKGDSRIPRSGNWQDLLHQLAIK"
     gene            complement(10334..11890)
                     /locus_tag="PKI07_08975"
     CDS             complement(10334..11890)
                     /locus_tag="PKI07_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC51223.1"
                     /translation="MRLVIVHHHFRPGGVRRVIELATPHLVSHWPEPIRTVVLAAGEA
                     PEAAWLRRFRKELPDVRVQVVVQPSFGYRSEMMETGSVWKRRVEEGISALVRLSMRED
                     CLVWAHNLGLGRNLELAHKLTQACHSGGVPLVAHHHDWWFENRWHHFTANRSPGFQTL
                     PAVANAVLPASPYIYHAAINRADAAVLRRHFHNQADWLPNPVMPPARVPARRVAAARA
                     WLQRQLNDKAPVWLLPCRLLRRKNIGEALLLTRWLRPQAWLVTTGDCSSTEETTYART
                     LADAARKGRWRLRLGVLRDRKTTSPSVPELLAACEAVMFTSLQEGFGLPYLEAVAARR
                     PLVARELPNIAPDLAEFGFAFPQSYREVYVAPELFDWRSERERQARRFARWIRVMPDA
                     AARLAGKPAVLAGGKKPRPVPFSRLTLAAQLEVLAQPIERSWERCLPLNSFLERWRQR
                     AAAGDLETAPWPPRAESWLSGHAYAQRFLSLVSPRRGEVPEPGAGQAAQAEFLRRKLH
                     ADYLYPLLWS"
     gene            complement(11896..12114)
                     /locus_tag="PKI07_08980"
     CDS             complement(11896..12114)
                     /locus_tag="PKI07_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51224.1"
                     /translation="MSPNYFRPDLEYAERIGETRDWEAFWTLANGIRRDMSLNHGLLR
                     AGLHLRISGYRLIKVYQEITRHRRESFL"
     gene            complement(12558..14108)
                     /locus_tag="PKI07_08985"
     CDS             complement(12558..14108)
                     /locus_tag="PKI07_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOC51225.1"
                     /translation="MNRNAPVLSNNLDALFLESRKCQARYDFAGAIEVLQRARRLEPA
                     NDKILVDLGAACAKAYDFAAAQRWFDEAIRVTLTPVPALNAIGHAWLEVRNYEAAQAC
                     FERLFKEKQVPVVAFIRLSEIYIRRRRLADADEITGRALHLYGPLDAVLLARGNVHRQ
                     LGQFDLAEKLYNVLVSRPAGDPLVRAFGYYELASLHDHKREYDKAMSALLEGKALMRQ
                     GAAQPLKILREKQAHLKEMQQSASEQAVQRWRKAGTTDLQPPRKLALLCGHARSGTTL
                     LEYVIDAHPGVVSAEESTVFHNKAYYPLGRAVSRNATFVSSLDWFPARTMRQIRSEYF
                     NGIESLLGEPIGDRLLLDKNPANTFDVPAIARIFPENKFVAALRDPRDVCLSCFMQPV
                     NILPDTAAWLSLEETVQHYAAIMGLWAAWKPCLGELAIEVRYEDLVDNLEASARRVLA
                     FLDLPWDDRVVRFHEHASSKVVRSPTFAEVTKPLYRASVGRWRNYQKYFEPHLPKLAP
                     LLRAFGYE"
     gene            complement(14323..14700)
                     /locus_tag="PKI07_08990"
     CDS             complement(14323..14700)
                     /locus_tag="PKI07_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51226.1"
                     /translation="MKISAWILTAVVATTLALVGCGKSGGGESVDTSALQASFESAEA
                     SVKESAEKAIAAVKSSDWAGAVTELKKLLEDAKLTAEQQHAVKDTLAKVQSAIGKVAG
                     EVTEGAEKAIDDAAKAVKELPKP"
     gene            complement(15043..16836)
                     /gene="dnaG"
                     /locus_tag="PKI07_08995"
     CDS             complement(15043..16836)
                     /gene="dnaG"
                     /locus_tag="PKI07_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOC51227.1"
                     /translation="MAGQLSRATREQIRAANDIVEVIGSYLPLKRAGANFVALCPFHK
                     EKTPSFNVNPQRQVFHCFGCHAGGDVFEFVKQYENIDFPEAVRRLAERARIPLTYEKS
                     DPGSYGRPLKEQLLQIHKQIAQRWQNALASEAAGQLARDYLAQRGVSAEAIQLFRLGA
                     APDLWDDTVNWAKSKGYELSLVEQAGLILRRTEGDGYYDRFRRRLMFPICDEQGRVIG
                     FSGRVLPGDEKAPKYVNSPETPIFTKSKVFFGLDKSRRAVLDAGYAVICEGQLDLIAA
                     YMAGVQNVVAPLGTAFTADHARILRRYVTEVVSCFDSDEAGQTAAVRSLDSLLESGLA
                     VRVAVVPSPHDPDSFIRAFGSAAFKQLIEGAEGFFDYYLHRLCATNEVASDKGRLAVL
                     RSMAEAVYKTGNAVLVDKYAQKTALRLGVTPDAVRAEFGKFSRSKTVRPEAAEESAPE
                     TAGPQKPSPSEFWLLKILLLHDDLVEWAAAHLDPAWVEHPVARQIIAQRLAAQTNQTW
                     LSLADFLDECDSPEMRSLVTEATTENRAIPNPSQQLKDIATRLRNQSIDRQLVALMQR
                     AHQPETNEADRLELLRQQQELRHCKRQPIQG"
     gene            complement(16846..18921)
                     /locus_tag="PKI07_09000"
     CDS             complement(16846..18921)
                     /locus_tag="PKI07_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HOC51228.1"
                     /translation="MIVGRTPRHLAVAALVLLMLLGDGGGKGAAAAAPKQRALDEWRS
                     LTGPGAPAPRVFVKGDRVRFYFQIGSHIEEFTANWDRHRLPSDGYRVNSAVLRLKQRM
                     AHMPDGERGWREATVIYGAEWARLTTNLVEALAPATAGQGIYCQGFLADRLLYRDAQG
                     TPRFASLDEAPPGVVVARQLSFEETLSTVARMVEQDLARNHPGETLFLLLAPNPRRFP
                     QPLLLDRQQRQCIWLIPASLYDRTERGLGLSTTAQGFRALLLESHGLALLKNPISSVA
                     RLADLGVQSVVRFVRVPFPKPGTRYPELSSGPGMDLRAWEEWLDRYTGTRQEEGSLDL
                     LIDGVQFFPRLKQAIADATNHIHFNIYVFDKDDVGISVADQLKQRASEIEVKVIFDRL
                     GAIGGGGAPPSTPLPADFSSPSSIYSYLRQDSRVRVRPFLNPWFSANHAKVLLVDGTH
                     AWLGGMNLGREYRYEWHDLMVELQGPVVSTLEEEFRRSWAHEGLLGDFAYVVALARGP
                     RPDAPPSPPGDWIKLRLLPTRTAWKPFSAAVQKAIGASRSYVYLENPYLFDKRVIVSL
                     ARARNRGVDVRVVLPRVNDLKIGGRSNLVLANYLLEHGVRVYFYPGMTHVKALLVDGW
                     AAVGSANLNHLSLRVCQEQNVATSDPAFAARLKRELFEEDFSRAYELTEPLSIEWMDF
                     LADLLLEGL"
BASE COUNT         3959 a         6056 c         5549 g         3801 t
ORIGIN      
        1 taaagcgccg cagccgaatc cgcatcacct gggatggact gggcaggggg cgagtttccg
       61 ccgtaataat cccccaagcc tgaccagcct cgactatact gactcgtcca ccagttcggg
      121 aacgtctccc gcctttcgag gaactgccat catcccgccc ggccttcccc tgcctccacg
      181 cggcggctaa caccgcgctt gacctcccgt ttcgagcgca agggcgtcac tctttcgtga
      241 aaggcttccc ttgccatgca gccctgtcta atatcctgcc tcaatgaaga cgctgacaac
      301 ctttctggtt ctagcgacac tggcgctctg cgcctttggc cgcgaggcgc aggcggccgc
      361 ccaagtcccc gcgcctgcca ggacctatcg caacccgctg ctgcccgagc gcgaaatagc
      421 cgatcctttt gtcctgcggt tcaacggcca atattatctt tattcgacca ccgatagccg
      481 gggttacgag gtgtttgtgt ccgaaaatct ggtggactgg aagaacaagg ggcgcgtgtt
      541 tcgcgacccg cgcggcggga attgggcccc ggaggtgttt cataaccagc gcggcgatgg
      601 caaattctac ctctactaca ccgacaacat cggcggcccc aggcgcggtc cgctggaaaa
      661 gcagattggc gtggcggtgg cagacagccc gctcggccct tttattgatc aggcggtgct
      721 ggccacgcac tcaattgacg cccatctgtt tcaggacgat gacggcaggc tcttccttta
      781 ctacgtggat ttaaacgaag gcttcaagat tatggttcag cccatggccg acccgatccg
      841 caaggtaaac gaacccaaag ttgtaatccg gccaaccgtg gaatgggaga ggcgcagcgg
      901 cgaggtgacg gaggggccgt tcatgttaaa gcacgatgga atctactacc tgatgtattc
      961 cggctccggg gccgacactc ccaattacgg cattggctac gccacgtcca aatcgcccac
     1021 cggtccgttt gtgaaatacg cgggcaatcc catcgcccag cgcggcggca aggtgtcggg
     1081 gccgggacat cattgtgtgg tggaaggccc ggacggaaag ctctggatgc tctaccacca
     1141 gaagtgggat accgaggtga actggcgccg ctttctggcg cttgatccgc tgtggttcga
     1201 cagccagggt gtcctgcatg tgaaagtcac ccgcgacacg gaccaaccgg cccccggcat
     1261 ccccgccaaa taagcaacac gcgccagccg ccgatttggc cctcaaggag ccttgttttg
     1321 caaggctccc gtccccccgg cttataccgg gcggctacgc cgcaggctgc ttcaactcgt
     1381 tcctggcctc cgcaacgatc ttctccaggc ggcggccgta ttcgacgtag ttctggcgca
     1441 tggccgcttc gttggcggcc ttggcctggg cgtcgtggaa gacgacaacc atgtgcggct
     1501 cgatggataa gcctccgttg tagccgcgcg acagggcgtc cttgagaatg tcgcgcactc
     1561 gggcccgccc ttcgccgggc cagttgtagt ccgcgtcgtc ctttgcggga ttccaggtgg
     1621 cgtccttaac gtggatgtgg gcgacatgat cgcgcacgtg cgtccagaac tcccacgggt
     1681 cctgtttggg ccagggcttt ggcttggaac gatcgggatt gaagatgggg ttggcggtgt
     1741 cgaagaccca tttgaggccg ggacacttgt cgaggagttc gagggcgtgc tgccaactca
     1801 taccgccgta gttcatgcaa ttctcgtgga cgggctgaag accggcgtcg aggaagcggt
     1861 cggtcacttc cttcacccgg cgaaagacct cggccgggat cacgcacgcg tcgtcttccg
     1921 gcttgaaact catgatgcgg acgtatttgg ccctggcgcg ctgcatgcgc gggatggcac
     1981 gcttgacttc cgcaagggtg atgtcgaagg gatcgctcag cttcttggcc cagttcatga
     2041 tggtggagcc gaagcaatag gcgtttatgc ccgccgcctc cagcttgcga acggcgaggt
     2101 cgaacgcctc gtccggtatg tcgtggaagt tggccttggc aaagccaggg acttcgacgc
     2161 cccggggctc aaggagtttc cagcccagtt cctgggtggc ctggatctgg ccgtctatgt
     2221 tgttagcggc ttcatcgccg atgcccatgt atttcatatc atttacgaat ctaaaacgga
     2281 cactctgaaa acgaacctgc tgggtattcg caatcggcac cccggctcga aggaatctcg
     2341 ccggaacgca tcataaacga acaaacagcc agccggttcg ataggcggcc agttgcgtcc
     2401 gctatatgat aatccggccg ctgccgcggg ctgaccctca agtgttcaaa gaccaatagc
     2461 aacttgcgcc ttgcgggcgc gatagggcac aaagcccagg aatacggctt cgtcgagcac
     2521 cggtttccag gcttcccctg gcggccctca ggcccaagca cctcgcgaat gtattgggcc
     2581 ttatcccggc tgacctttcg ccgtcatcca ggcgcttcat ccattcgggc caccgggtcc
     2641 gacaggcagg atcatcagcc ccggccccgc acggcaagca agcggatgcg aagaatcgcg
     2701 ccgcttctgc cgcccggccc atgcgggctt tgattcccgg gatgctcatg cgcgacaccc
     2761 taactcctct gccccgattg acaagctgtt ttcttaccgc cggaaggact tggtgaagtc
     2821 ctcattagcg attgccacta ccttcttctt cacctttgac gctgcgatca attggttcag
     2881 cttcttctcg taggcccggc tgtccatggg caactccagc gttttcccca ggagcgagga
     2941 aaagaggatc gcgttggcca gttcgacgga atggatgcct tccgggcccg gcgtaatcag
     3001 cggctcgccg tcgaggatgg cgttgatgaa gttctgcatc aggaccgcgt ggccgttggg
     3061 agaagggccg aaggggattt cgacgtgcca gacctcgggc ttggcgaagc cgagcctggc
     3121 cgtgcggctg aactcgatca tatccacctc gttgcgctcc agggtgagct tgtccgcttc
     3181 gagcagcagt ttgccgcgcg tgccaacgat ctcgaagcgg ttggtgccgg gggactcgcc
     3241 ggtggaggag acgaacgtgc cggtggcgcc gttcggatat tcaaggtagg cggagacatt
     3301 gtcctcgacc tcgatctgat ggaagcggcc gagctggcag aatccgcgca cgcgggccgg
     3361 catgccgagc agccattgca gggtgtcgag gttgtggagg cattggttga gcaatacgcc
     3421 ccctccctcg cccttccatg tggcgcgcca gccgccgctg gcgtagtagg cctcggtgcg
     3481 gaaccaatcc gtcatgaccc agctcatgcg cacgatctcg cccagttcgt tgtcctgaat
     3541 gagcttgcga atcttcaggt aacgcggctc ggtgcgcagt tggaacatgg cggcaaatac
     3601 cagcctgggc tttgtcttat gcacggcgat cagccgctcc gcgtcggctt tgtgggcgga
     3661 gatgggcttc tcgaccatca cgtgcaaccc ttgtttaagg gccgcaatcc cgagggtggt
     3721 atgctggtaa tgcggggttg caatgatgac cgcatccacg aggccggagc ggattaactc
     3781 ctccccgccg atgaaggttt tcagcggttt gtatttctcc agcttggccg aggcggtatc
     3841 tgacaccgct accagttccg cccggctgat tttgccggcg gtaagatagt taaaatggtg
     3901 ctggccaatg ttgcccaacc cgataagtcc caagcgcacg tgcttcatac gaaagagctt
     3961 aacacaggat tgccgcttgc cgagtgaaat agtgtaagcc ccggcgcgcg caacggcccg
     4021 gcccccgcat ccgtgccggg cttatgccgg tcacatcagc catgacagta tcggggcaaa
     4081 aatcaagctg ggcaggtaat cggccagttc gattttcttc aagtcaagga ttaccattcc
     4141 cacacagaag accagcagtc cgcctgccgc attgaccggg tccaccaggc tgtgggcctc
     4201 cagaaaaggt ttaagcccat gcagacacaa caacgtaatc gtgccctgaa aagccagcac
     4261 cggcagggca accagcaaca ccccccagcc gaacacgaga gtaaacccca tcgtggccag
     4321 gccatccatt accgccttca ccgcgagggg gtaatagtag ccgctcaaac cgtcttgcac
     4381 cgaacccagg atggccaatg gcgcgacgca atagagcgcg gcgcaggtct tgaacccctc
     4441 gcccaggcgg cccggcgcgg caggtcgggc cgcggctatg ctctcgcgcg cctttttccc
     4501 cacccggttc gacatcttct gcagacgcag caatcgcccg gttaacttcc ccagcattag
     4561 cgccagcaca acaatgagaa gctgccggag gatttgccac aaggacccat ttagactggt
     4621 ccaggtcagc cgcagaccat agaaaatggc gaagactccc agcgcaacct tgaaaaagga
     4681 ttcccgcgcc ggggagaaag gcttgcgccg cgccaaacca atcgcgccgc caagcagaat
     4741 ccccgcaaca ttcagagctg tgccgatcat tcaagacagg ctagcggcta taatccaaag
     4801 tccaaagccc aaagtctctt tcggaagcgc ctgcgtgtgg tcttacggcg aaaagactcc
     4861 ggcaagaacg agatgagatt ggccgggctc agaatactcc tcgtgcgatg ctaatccccg
     4921 acgaaccgcc tcctcaaaaa aattgataat aacgcggcct tgacggcttt ggccattcag
     4981 cggcatagtt atcaggctcc taacgcaaac gcataagcgt tgactttgag cgcaagggtc
     5041 cgacggtggg tgccgtcgga ggataagtcc gaatggtcgg gccgccatcg ggataatcag
     5101 acgactcgac gccgatgcgg ccgcaaacag cgcgcgcccg cggcagagac gcaaaacgca
     5161 aacaaccatg cctgactttt ttcaaaccgg cgccatcgcc accctgcatc ggctggggcg
     5221 ccccgacgtt gcccggctcg agcatgagtt ggagcgattt gcagccgaat cgccgattac
     5281 gttggtattg ccgtgccaca tcaaagaact cggcacgcgc gcgttggcgc ggattgtccg
     5341 cgaactcaag aacgtttcct acctcaagca aattgtggtg ggcatcgatg gcgcgacccg
     5401 cgcggctgat ttccagcgag cctgcaaatt cttcgcccag ttgccgcaac gcccgatgtt
     5461 attgtggaac gacgggcctc ggctgtcagc gctattccaa aaacttgagg aagccgagtt
     5521 gagcgccggg ccgggcggca aagggcgcaa tatttggatc tgcttcggct atgtgctggc
     5581 cagcgaacag gcgcgcgtgg tcgtggtgca tgattgcgat atcctgacct acagccggga
     5641 gttgctggcc cggctaactt acccggtggc gcaccccagc ctgggctttg atttctgcaa
     5701 aggctattac gcccgcgtca ctcaccagtt gaatggccgc gtgatgcgcc tgctggtcac
     5761 gccgcttctg cgggcgctca aaagcattat cggccagcat ccctacctgg tctatatgga
     5821 taccttccgc tatccgctgg ccggtgagtt ctcaatccat accgacctgg tccggcgcgt
     5881 gcgcattccc tacgattggg cggtggaggt gggcaccctg gccgaggtgt ttcgcaacgc
     5941 cgcgccccgc gcgatttgcc agtcggaatt atgcgagaat tacgaccaca aacatcagga
     6001 gctttcgccg cgcgacaccg ccaaggggct taacaagatg gcggtggata tcaccaaatc
     6061 tttcttccgc cggatggccg ccgaaggcat caaactggat gtggggctgt ttgacaccct
     6121 tttgagcgcc tacttgcgcc aggccgaaga ctcgcttcga ttctatggcg ccgatgccgc
     6181 cgtcaatggc ttgtcgtttc cgcggcatga ggaggaaaaa gcggtggcca cattcgtgcg
     6241 cagcatccaa attgcggaaa aggctttcct ggaggacccg ctgtcgactc cgctgattcc
     6301 caactggaac cgcgtgcaat ccgccctgcc caggttcttt gatgggttga acgctgcggt
     6361 tcggctggat aatacgcatt gaatggcggc attatgatgc tcgaccgttc aactggcagc
     6421 ggcaaaccag cgcgcacctg ccggcatttt gggtaaaccg tagcgcatga cttcacagtt
     6481 ccgccagtat ctggatttcc tctatggacg ggagggcgcc gctgcttcca tgcggcaact
     6541 gtccgtactg ctaaaatcct ggccgcgaca gacgctcggc agattccgcc gcacggtaaa
     6601 gccactcgcc gagcacgata tcctgcttat cacctatgct gaccaattgc gagaacccgg
     6661 ggaagcgccg ctgcggacgc tggctcgttt ctgcaaatca catttgcaag gtttgattag
     6721 cggcgtgcat atcctgcctt tttatcccgc gtcatcagat gatggatttg ctgtaaaaga
     6781 ctacttcgcg gtggacccgg cattcggctc gtggaatgac gtggcgcgcc ttgggcagtc
     6841 gtttgatttg atgttcgacg ctgtcgttaa tcacctttcg gcgcaagggg aatggttccg
     6901 ccgttttcag cgcgatgatc ctcaattccg ggatttcttt ataacggtca agggacagcc
     6961 ggacttgtcg caggtcgtgc gcccacgcac cctgccgtta cttacggaat ttcccactgc
     7021 gcgcgggcgg cgccggttat ggacgacttt cagcgccgac caggtggatc tggattaccg
     7081 gaatccccgc gtactgctgg ccgtattgga agtgctgctg ttttacgcgg cacgcggggc
     7141 gcggttcatc cggttggatg ccatcgcgtt tctctggaag gaaatcggaa caacttgcct
     7201 gcacctgccg caaacacacg caatcgtcca gcttatgcgg gcggtattgg ataaagcggc
     7261 tccgcatgtg cggctcatca ccgaaaccaa cgtgccgcac gctgaaaatg tttcttactt
     7321 tggaaacggc gccaacgaag cacagttggt ctataacttc gctctgccgc cgctggtgct
     7381 gcatagtttc gccaccggca acgcggtgaa actgactcgc tgggcgcagt cactaacgtt
     7441 gccgtcggac cgggtggcgt tcttcaactt cctggcctcg cacgacggca tcggcctgaa
     7501 tccagtgcaa ggcatcctca ccaaggcaga aattgaggca ctggcaagcc gcgccctggc
     7561 gcacggcggc ttcctttcat tcaaggagtt gccggatcac acgcaggttc cttacgagtt
     7621 gaatattaat tatctcgatg ccctgtcgaa cccgcggcga ggtgaacctg ccgaattggc
     7681 cgcccgcaag tttctcacgg cgcatgccat tctgctcagc ctgcaaggag tgccgggcat
     7741 ttatttccac tcgcttctgg gttcacgggg cgaccgggcc ggcgcggaaa ccagcggctt
     7801 gaaacggcgc atcaaccgcg agaagttggc tcgcgcgcaa ttggaaagcg agttggccga
     7861 agcaggctcc ctgcgagcct gcgtttttgg cggattgaac aagctgctgc gtattcgccg
     7921 ccaacaccgc gcatttcacc cccaggcggc gcagcgggtg ctggcgggcg atcggcgttt
     7981 gtttgccgtg tatcgggctt cgctcgatgg ccgtcaacgg ttgctgtgtt tgcacaacgt
     8041 taccagccaa acggtttccg cctctcgcct ggtgcaagcg gctgagtgcg cccctttctg
     8101 gactgaactg atctccggcg accgctatca gccggcaaac gtacggctca agggatacca
     8161 gaccctttgg ctgcgcgcgg aagacaaata atcgcgcccg ctaaactcct tctccaaaca
     8221 ggttggaaat gcgggcagcc agtttgcgcc gcgccacaga ataagagaag tatttcacgc
     8281 ccagagcgta gttggtgcgc gcccactctg ccgccagcgc ctggtcctcc aagacggccc
     8341 gggcttgttc gaccacctcc cgggtcacca actgtggcat ctcgatggtt ttaaacccca
     8401 gcggatcaat atcgcgcgca tacacggcgt aagtgtttac gactaccggc ttgctaaaat
     8461 aaaccgcctc caaaaaggcg ttgccgaatc cttcataatg actggggtag gtaatcaggt
     8521 ccgcgtgcgg atagacatcg gccagggtgt aaatcttctt tccctccgga gtggtgccgc
     8581 gggtttcgcc tacccgatcg aaaatgaacc gcacgtcaat gccggcatcg gcaatacggt
     8641 ctttgagcat ggccatgtaa gcgttccctt cgtcgcctgc ggggtgcgag atgacgagct
     8701 tggcccgcga attattaagg cggcgcacca gttcgatggc atgctcgatg cctttgcgct
     8761 gaaccacacg cgtgggctgc agaataaacc aatcgctctc ttccaagccg atctcacggc
     8821 gaagatcccg gctatagtca tcaacacccg gagggggcgt ttcgaagtcc aacacattcg
     8881 ggatgatttg cgaaggaatg ccgagccgcc gggccagttc tttttgctgc atcgagttga
     8941 tcaccacgtg ctccattttg tagaatagcg gcgggaaggc agcttgcaga taatcgctaa
     9001 cggcattcaa cgtgaagcgc tcgcgctccc aggcaaaatc gtgatgatgg gcgatgacgg
     9061 gcaaggtggt ctcggccgtt acctcggtca tggccagccc tagcggcacg tgcatcggga
     9121 tggccagaat gttttccggc agaaggattt caatctggaa cttctctatg aagcgataaa
     9181 gctcatcctt cagccgttct ttaatggatt ggatttgatt ggtgacagcc cggcttcgaa
     9241 acgccacgcc aaaaagcctg ctctgcactt cggcaacctc cggatggcca aagaaggcca
     9301 gcggtgcaac atggctcgcc tccggagggg catcgagcaa tcccgccatc caaaaacagg
     9361 agtgccccat cccggtcaga atctgcgccc attttcgcgt ttcgagcgtg acaccgtccg
     9421 taccatgaaa ccgtgtggag atgaagccga tgcgtttagg cgcgcccgtc gtaagtatgc
     9481 agaatgacat tagtcgatgc taccgccact actcggaact tcaagcctga cgcaaagctc
     9541 cggcgcggaa tagttaccgt atttccgacg gcaaatagcc ggttccgtca agtcggcctg
     9601 atttatttta tggcgagctg atgcagcaga tcctgccagt ttccgcttct tggaatgcgg
     9661 ctgtcgcctt tcggtttcaa tagccacgtc tgccagccaa aggcgcgagc cggctcacag
     9721 tcgttatcga ggcgatcgcc aaccataagc gcctcggccc gggaaatgcc ccgtgcctcc
     9781 aaccgggtgc tgagaatctg gaacacatgc gggtctggtt tgctaaagcc atgctcgaac
     9841 gaccagaagc aaaggtcgcg ctcgaacagg tccatcccaa gcccatggcg aaccagggcc
     9901 tcttccaatt cgcgcagtgt gtatgcctgg gcattcgaag ctatgccgaa caggcagccg
     9961 ctcgccttga gtttgcgtaa ggccgctacc gtagcatcac tcaggagtgg agtgtggcct
    10021 atctggattt gacgatagag aaattcgtcc agtttcgccc gcggcagccg gcgcaagggc
    10081 ggcagtactt ccgcgaccac cgcaggccac tgcacctccg gccaggctat gccgcgcgcc
    10141 cggctggctt catggcgttt ggccacgagt ttgttgctgg ccacatagaa atccaggcgg
    10201 ctcaacaaag gaggggcgtg cagcatctct tgaaatagct gctgccaacg cgcatcggca
    10261 tccaccggcg gcgggccgat ctccaaaagg gtgccataaa catcgaaaat aaccgcgcgc
    10321 acattcacat ctgctagctc cataaaaggg ggtaaagata atcggcatgt aatttccgcc
    10381 gcaaaaattc agcttgcgcc gcctggccag ctccaggctc cggaacctct ccgcgccgcg
    10441 gcgaaaccag agacagaaat cgttgggcat aggcgtgccc gctgagccag gattccgccc
    10501 gaggcggcca gggtgcagtt tccaaatccc cggcggccgc ccgctggcgc caccgttcca
    10561 gaaacgaatt aagtggcagg caacgctccc acgaccgttc aatcggttga gccaatacct
    10621 cgagttgagc cgcaagcgtc agtcggctga atggcactgg ccgcggcttc ttcccgccag
    10681 ccagcacggc gggtttgccg gccaggcgag cagccgcatc aggcatcaca cgaatccatc
    10741 gcgcaaacct gcgggcctgc cgttcccgtt cgcttcgcca atcaaacagc tccggggcca
    10801 cgtatacttc gcgataggat tgcgggaaag caaacccaaa ctctgccagg tccggtgcta
    10861 tgttcggcaa ttcccgcgcc accagagggc ggcgagctgc caccgcctcc aaatacggca
    10921 aaccaaaacc ttcctgaagc gaggtgaaca ttaccgcttc gcaggcggcc aacagttccg
    10981 gcactgaagg gctcgtagtt tttctatccc gcaaaactcc aagccgcagc cgccagcggc
    11041 ctttgcgcgc ggcgtcagcc aacgtccgcg cataagtggt ttcctctgtc gaactgcagt
    11101 cgcccgtcgt gaccaaccat gcctggggtc gcaaccagcg ggtgagcagc aaggcctcgc
    11161 caatgttttt acggcgcaac aaccggcaag gcagcagcca gacgggagcc ttgtcattca
    11221 gttgacgctg aagccaggca cgggcagcgg caacgcgccg ggcgggcact cgcgcgggcg
    11281 gcattaccgg attgggtagc caatcggcct ggttgtggaa gtgccgccgc agcactgcgg
    11341 catcggcgcg gttaatggcc gcatgataaa tatacgggga ggcaggcagc accgcattcg
    11401 cgacagccgg caatgtttga aaaccgggac tgcggtttgc ggtaaaatgg tgccaccggt
    11461 tttcgaacca ccaatcgtga tggtgcgcca ctagcggaac acccccgcta tggcatgcct
    11521 gcgttaactt atgcgccagt tccagattgc gccccaaccc caaattatgc gcccagacca
    11581 ggcaatcctc acgcatcgag aggcggacca gagcgctaat gccctcctcg acgcgccgct
    11641 tccaaaccga acccgtttcc atcatttcag accggtagcc aaaggatggc tgcacgacga
    11701 cctgaacgcg cacgtccgga agctccttac ggaaacggcg cagccaggca gcttccggag
    11761 cctcgcccgc ggccagcact accgtgcgaa tcggctccgg ccagtgcgac accaaatgcg
    11821 gcgtggccaa ttcaattaca cgccgcaccc ctccaggccg gaaatggtgg tgaacaatga
    11881 cgagtctcac ggattttaaa ggaaagactc gcgtcggtgc cgggtgatct cctgatagac
    11941 cttgatcaag cgataccccg aaatgcgcag atgaaggccc gcgcgcaaaa gaccgtgatt
    12001 taagctcatg tccctgcgaa tgccattggc cagcgtccag aatgcctccc agtctctggt
    12061 ttcgccgatt cgctccgcat attccaaatc cgggcggaag tagttgggcg acaagctccg
    12121 aaccaaccaa gccaggggac gcaaaggagg atggatgctc tttcttaagg gcgcctttgg
    12181 gaaggcctca actgagcacc cgaaacattc gcaaaagccc accttaaact ttcggggctg
    12241 cgggactgaa tgtttgctgg cgtccacgcg tttagattag ccagccaccg caacccttgc
    12301 aagctcacag catggagtgt tctcgtaaaa cccagtcatt gcgaactgtt cctggcacgc
    12361 gcaaacctgg aaccgcgctt gcctttgtct gaagaaccag acgggccgag tccgtaccgc
    12421 caaagatttt ccaaaacgct tcgcagaaac ccgtgatgaa ggaagaacac ccctccgacg
    12481 cgaactggca accttacgca agccaacctc ctcctcggcc tgcaccaaac gacccgccca
    12541 tggaaaaccg tccttcctta ttcgtagccg aatgcgcgca gcagaggtgc gagtttgggc
    12601 agatgtggct caaaatactt ctggtagttc ctccagcgcc caacggatgc tcgataaagc
    12661 ggcttggtaa cctcggcaaa ggtaggcgaa cgaaccactt tgctgctggc atgctcgtga
    12721 aagcgcacaa cgcggtcatc ccaaggcaag tccagaaatg ccaacacccg ccgggcgctg
    12781 gcttccaggt tgtccaccaa atcttcgtaa cgaacttcga tggccagttc cccaagacag
    12841 ggtttccagg cggcccataa acccatgatg gctgcgtagt gttgcaccgt ttcttccagg
    12901 ctcagccacg cggcggtatc aggcaggatg ttcaccggct gcataaaaca gctcaggcaa
    12961 acatcccggg gatcgcgcaa ggcggccaca aacttattct ccggaaaaat gcgagcgatg
    13021 gcgggcacat caaaggtgtt cgctggattc ttatccagca gaaggcggtc gccgatgggc
    13081 tcgcccaaaa gggattcaat cccgttgaaa tactcggagc ggatttggcg catggtgcgg
    13141 gcgggaaacc agtccaggga ggagacgaag gtcgcgttcc gggagaccgc ccggccaagc
    13201 gggtagtagg ccttgttgtg aaagaccgtg ctttcctcgg ccgaaacgac gcccggatgc
    13261 gcatcaatca catactccaa aagggtggtt ccggagcggg catgaccaca aagcagggcg
    13321 agcttgcgcg gaggttgcag atccgtagtt cccgctttgc gccagcgctg taccgcctgc
    13381 tcactggcgc tctgttgcat ctctttcaga tgcgcctgct tctcccgcag aatcttcaac
    13441 ggctgcgcgg ctccctgccg cataagcgcc ttgccctcca gcaaagcgga catcgcctta
    13501 tcgtattccc gtttatgatc gtgaaggctg gccagctcgt aatagccaaa ggcgcggacc
    13561 aggggatcgc ccgcggggcg gctcacgaga acattgtata acttttccgc caggtcaaac
    13621 tgccccagtt ggcgatgcac attgccgcgc gctaataaga cggcgtccaa gggcccgtaa
    13681 aggtgcagcg cgcgcccggt aatttcgtcg gcatcggcca gacggcggcg ccggatatag
    13741 atttcggaca ggcggatgaa cgcgacaacc ggaacctgct tttccttaaa tagccgttca
    13801 aaacaggctt gcgcggcttc gtaattgcgc acctccagcc aggcatggcc gatggcgttg
    13861 agagcgggaa cgggggtgag cgtaacgcgg atagcctcgt cgaaccagcg ctgcgcggcg
    13921 gcgaaatcat acgccttggc gcaggcagcc ccgaggtcca ccagaatctt gtcattcgcc
    13981 ggctcaagcc tgcgggcgcg ctgcagcacc tcgatggccc ccgcaaaatc ataacgggcc
    14041 tggcacttcc tggactcgag aaacaacgca tccaggttat tcgaaagcac gggcgcgttt
    14101 cggttcatgg cgcgagtatc cgggcgcgac aggcgcaaag acaagcagca tccctccggc
    14161 gcgagcgggc ttggaagcaa tcccgggtca tcttccccaa gccttgcggt ccggcggccg
    14221 cgtgaaagca aatccgtaaa cagaagtggc gcgtgacctc aaaacgccac gcgccacggt
    14281 gaagatatga tttgcgtcca acgaacggct ggccggggcg gtttacggct tcggcaattc
    14341 tttgactgcc ttcgccgcgt catcaatcgc cttctcggct ccctcggtta cctcgcccgc
    14401 cactttgccg atcgcgcttt gcaccttggc cagcgtgtct ttcaccgcgt gctgctgctc
    14461 ggccgtcaac ttggcgtcct caaggagctt cttcaactcc gtcacggcgc cggcccaatc
    14521 agaggacttc acggcggcga tcgccttctc tgcgctttcc ttcacgctgg cctccgccga
    14581 ctcgaagctc gcttgcaggg ctgacgtatc cacactttcg ccgccgcctg atttaccgca
    14641 ccccaccagc gccagcgtgg tggccactac tgctgtcaat atccatgcac taattttcat
    14701 actccgccct ttctatgttt gcgttgtttg tttggctttc aggttcgtta attcctgtct
    14761 atggctggca ctttaacgcc gcgcccgccc agagtcaata cggatttgaa attcctcatc
    14821 tcccttgctc agggaaagcg ctttgcatcc ctcttgaagc cagcactgcg caacacacct
    14881 gcgaaccctt tgtggaaaag cgcttgcgtg actcaggaaa ctctcaaacg gaaccgcttc
    14941 gaacggaagt tcgtccccct cgctctcaac aaggaacggc gtcaacgcac cccaaaaaca
    15001 accgagcggc cggccgacgg cacgttgccc gggtgccagc ggctacccct ggattggctg
    15061 acgcttgcaa tgccgtaatt cctgttgctg gcgcaacagt tcaagccggt cggcttcatt
    15121 ggtttccggc tggtgagcgc gctgcatcaa ggcgacaagc tggcggtcaa tgctctggtt
    15181 ccgcaaccgc gtggcgatat ctttgagctg ctgagatggg ttgggaatgg cacggttctc
    15241 ggtcgtggct tcagtcacca ggctccgcat ttccgggctg tcgcactcat cgaggaagtc
    15301 tgccaggctg agccaggtct ggttcgtttg agcggccagg cgctgcgcga tgatctgcct
    15361 tgccaccggg tgctccaccc aggccggatc caaatgcgcc gccgcccact ccacgaggtc
    15421 atcgtgcagc aggaggatct tcagcagcca gaactccgaa ggcgatggct tttgcgggcc
    15481 ggcagtttcc ggggccgatt cttcggcggc ttccggtctc acggttttcg atcgggagaa
    15541 cttcccgaac tcggcacgga cagcgtctgg cgtcaccccc aggcgcaaag cggtcttttg
    15601 cgcgtattta tcaaccagca ccgcgttgcc agtcttatac acggcttcgg ccatgctgcg
    15661 cagcaccgcg agacgcccct tatcggaggc aacctcgttg gtggcgcaga ggcgatgcaa
    15721 gtagtaatca aagaagcctt cggcaccttc aatgagttgc ttgaaggcgg cactcccgaa
    15781 ggccctgata aagctgtccg ggtcgtgggg cgatggcacc acggccacgc gcaccgccag
    15841 gccggactcc aggagagaat ccaacgaccg aaccgcggcg gtttgaccgg cctcgtccga
    15901 gtcgaagcaa ctgaccacct ccgtcacata gcgccgcaga atccgggcgt ggtcagcggt
    15961 gaaagccgtt cccaggggag ccacgacatt ttgcaccccg gccatatagg cggcgatcag
    16021 atcaagctgc ccctcgcaaa tgaccgcata tccggcatca agcaccgcgc gccgggactt
    16081 atccagccca aagaagactt tgctcttggt gaaaatgggt gtctcgggcg agttgacgta
    16141 tttgggcgcc ttctcatccc cgggcagcac ccgcccactg aagccgatca cccgcccttg
    16201 ctcgtcgcaa atggggaaca tcagccgccg gcgaaagcgg tcatagtacc cgtcgccttc
    16261 cgtgcggcgt aaaatgaggc cagcctgctc caccaaagac agttcgtagc ccttgctctt
    16321 cgcccagttc accgtgtcgt cccacaagtc cggcgcggcg cccaaacgga agagttggat
    16381 ggcttccgcg gagacgccgc gttgggccag gtaatcccgc gccaactgac cggcggcttc
    16441 gctggcgagg gcgttttgcc agcgttgggc gatctgctta tggatttgca acaactgctc
    16501 cttcagcggt cgcccatacg acccggggtc gctcttctcg tacgtcagcg gaatcctcgc
    16561 ccgctccgcc agccgccgca ccgcctccgg gaaatctatg ttttcgtatt gtttgacgaa
    16621 ctcgaacacg tcgccgcccg catggcagcc gaaacaatga aatacctggc gctgcgggtt
    16681 gacgttgaag ctgggcgtct tctctttatg gaaggggcaa agcgcgacga agttggcccc
    16741 ggcgcgtttg agcggcaggt acgatccgat cacctccacg atgtcattgg cagcgcggat
    16801 ttgctcgcgg gtcgcccggg aaagctggcc agccatgacg acagcttaga gtccttcaag
    16861 cagcaagtca gccaggaaat ccatccattc gatggacagc ggctcggtca gctcatacgc
    16921 gcgggagaaa tcctcctcga agagctcgcg cttgaggcgg gcggcaaacg cggggtcgga
    16981 ggttgcgaca ttctgctcct ggcacacgcg gaggctcaag tggttcaggt tggccgatcc
    17041 caccgcggcc cagccatcca ccagcagggc cttcacatgc gtcatgcccg ggtaaaaata
    17101 aacccgcacc ccgtgttcca gcagataatt ggccaacacc aggttgcttc gcccgccaat
    17161 cttgagatcg ttcacccgcg gcaacaccac gcgcacatcc actccccggt tgcgcgcccg
    17221 cgccaggctg acaatcacgc gcttgtcaaa caggtaggga ttctccaggt aaacatagct
    17281 gcgggacgcg ccaatggctt tttggaccgc ggcactgaat ggcttccagg ccgtccgggt
    17341 gggcaggaga cgcaatttaa tccagtctcc cggcggtgac ggcggcgcgt ccggtctcgg
    17401 cccgcgggcc agcgccacca cgtaggcaaa atctcccagg aggccctcgt gggcccaact
    17461 ccgccggaac tcctcctcca gggtgctcac aaccgggcct tgcaactcga ccatcaaatc
    17521 atgccattcg tagcggtatt cccgccccag gttcattccc cccagccacg catgggtgcc
    17581 gtccaccaat aacaccttcg catgattcgc cgagaaccac ggattcagaa acgggcgcac
    17641 ccgcacgcgg gaatcctgcc gcaaatagga atagatcgag gaagggctgc taaagtccgc
    17701 cggcaacggc gtgctgggcg gggcgcctcc tcccccaatc gctcccagcc gatcaaagat
    17761 tactttgacc tcgatctcgc tggcccgctg ctttaactgg tccgccacgc tgatgccaac
    17821 atcgtccttg tcgaaaacgt agatgttgaa atggatatgg ttcgtcgcat cggcgattgc
    17881 ctgcttgagc cgcgggaaga actgcacccc gtctatcagc aggtccagcg agccttcctc
    17941 ctgccgcgtg ccggtgtagc gatccagcca ttcctcccac gcccgcaaat ccattcccgg
    18001 ccccgaactg agttcgggat agcgcgtgcc gggtttgggg aacggcacac gcacaaaacg
    18061 aaccactgac tgaaccccca aatccgcaag ccgggccacg ctggaaatcg ggttcttgag
    18121 cagcgccagc ccgtggcttt ccaacaacag cgccctgaat ccctgcgccg tcgtcgaaag
    18181 ccccaggccc cgctcggtcc ggtcatagag cgacgccggg atcagccaga tgcactgccg
    18241 ctgctggcga tccagcaaaa gcggctgcgg aaagcgccgg ggattgggcg ccagcagcaa
    18301 aaaaagcgtt tcccccgggt gattccgcgc caggtcctgc tcgaccatcc gcgccaccgt
    18361 cgagagcgtt tcttcaaaag aaagctgccg ggccacgacc actcccggcg gcgcctcgtc
    18421 cagcgaggca aaccggggcg tcccctgcgc atcacgatac aggagccgat ccgcaagaaa
    18481 cccctggcaa taaatcccct gcccggcggt ggcgggcgcc agtgcctcca ctaaattcgt
    18541 ggtgaggcgg gcccactccg ccccgtaaat caccgtcgcc tcgcgccaac cgcgctcgcc
    18601 gtccggcatg tgcgccatcc gctgcttcaa gcgcagcacc gcggagttga cgcgataccc
    18661 atcgctcggc agccgatgcc gatcccagtt cgccgtgaat tcttcaatat gggagccaat
    18721 ctggaaataa aaccgcaccc ggtccccctt cacaaacacc cgcggcgcag gcgcgccggg
    18781 accagtgagg cttcgccact cgtccagcgc ccgctgcttg ggggctgccg ccgccgcgcc
    18841 cttaccccct ccatcgccca ggagcatcag caaaaccagc gccgccacgg ccaaatgcct
    18901 tggggtgcgt ccgactatca ttggttaaac agtcaacaat cttgccccca tgaagtcaac
    18961 cgccagaagc ctgcagcatc ccctgattct tacctctcgt ttcaggagtg tccaattttt
    19021 ggacaccccc aagcagtacg ttcctggctc tcctgcgcct cctaaagtca ctgtcatctc
    19081 caaacccgtt caactcctat catttcaagc acatctatag ctaacctcaa gccgtgtaca
    19141 aggggtgtac aagggatgct catcggatgt actaactaaa atcctatgaa ctcccgggga
    19201 gcatcccttg tacacccctt gtacacggct atttgggacg gcaaaagaag ggtatggcaa
    19261 gggaggcgga cgcctttgag agctatgatt ctggggcctc gggaagccag ggttcacaag
    19321 gggacctgtt ctctcagtat ggagtagcct aatgtttttg ggcgg
//
LOCUS       DAOPQG010000069        19241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3475_length_19241_cov_15.962681, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000069 DAOPQG010000000
VERSION     DAOPQG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 19241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19241
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3475_length_19241_cov_15.962681"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1074)
                     /gene="rsmA"
                     /locus_tag="PKI07_09005"
     CDS             complement(196..1074)
                     /gene="rsmA"
                     /locus_tag="PKI07_09005"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOC51229.1"
                     /translation="MKLTDMRRVLAARGIRLTKSLGQNFLHDANQLRRIVAAAELTRA
                     DRVLEIGPGLGPLTERLRARAGEVLAIEKDAQLARFLQERWPPGSPAAGALRILTDDA
                     LEFLRREPRDWRQWKLVANLPYSVASPILVELAGDRGPQRLVATLQLEVARRLMAQPG
                     SPDYGVLTLLVQLNCQPQTWFRIPAACFFPEPDVDSACVCLVRRPRPLLPPESRDAFV
                     RIVKRAFSQRRKMMFKLLKADWPQPRLAALFRQLDLSPQVRAETVSLEQFAHLAAGLH
                     PGSPAPAPAPQRGALR"
     gene            complement(1094..2416)
                     /locus_tag="PKI07_09010"
     CDS             complement(1094..2416)
                     /locus_tag="PKI07_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOC51230.1"
                     /translation="MTTRQTLARLRAYESRNVTFLDPDGAWPIVWERARGTHVWDPEG
                     RKYLDLTAAFGVAAAGHANPRVTRAGQRQMTRLLHAMGDVHPHALKAQLARELSRVTF
                     ERWTRTLRPRPPAPARTGKTLFCNSGFEAVEAALKTARLATGQPGVIAFTGAYHGLGY
                     GALNVTARAHFRAPFRSQLRQFGRFVPFPPARPAAALADLESQLRRQFRDGRVGALLA
                     EPIQVRGGVNLPPPGFLPLLRRLCDQHRALLILDEIYTGFGRTGKWFACEHARTIPDV
                     ICLGKALTGGFPLSACVGRADLMDAAWPPSDGEAIHTSTFLGHPVGCAMALAQIREIR
                     RLNLPRRSARLGRVLLAALAALARDARGAQLRVRGQGLLAGVEIRHADGSPAPRRALA
                     LVKTLLRRGFIFLPEGDPPNVISFTPPLTISARQLRTAVAALRRECAA"
     gene            complement(2424..3203)
                     /locus_tag="PKI07_09015"
     CDS             complement(2424..3203)
                     /locus_tag="PKI07_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC51231.1"
                     /translation="MLAPMSDTLVVNEIYLSLQGESTFAGLPCIFIRLTACDLRCSYC
                     DTAYAFTAGTRRSLAEIRREVRRLARPFAPPAARRALRARRPAAAERLPLVELTGGEP
                     LLQKNSLPLLRALCDDHFTVLLETNGARDISPVDPRVRRIVDLKCPSSGEAARNRWEN
                     LACLRPTDQLKFVIASRQDYQWAKACIARRQLAARCPLLFSWAQPLAPAQRHPALKPV
                     PPGHTPLSRRELAEAIIADALPVRFQIQLHKVIWAPAARGV"
     gene            3291..4547
                     /locus_tag="PKI07_09020"
     CDS             3291..4547
                     /locus_tag="PKI07_09020"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOC51232.1"
                     /translation="MDIYDHPSFRMACQQFDLVADHLQMPESERARVKYPKRAVTVAL
                     PIHRDDGSVQVFPGYRVQHHLALGPTKGGLRFHPDVTLGEVAALAMWMSWKCALMGLP
                     YGGAKGGIACDPAQLSQAELERLTRRYTQEMIPFIGPQIDVMAPDLGTNEQVMAWMMD
                     SYSVHIGAAVPSIVTGKPVALGGSQGRREATGRGVGYLVNRATDTIGLDISKATAAIQ
                     GFGNVGSVAALSLARYGVKVVAVSDVFGGVYNANGLDLWGLEKHVAAQKTVVGFSEAE
                     PITNEQLLVLPCSILVPAALERQITAANAGQIQCRIIAEAANGPTTPEADEILNQRPE
                     IFVIPDILCNAGGVVVSYFEWVQDLQSFFWGETEVVDKLFRLLESAFTRVVSLSRKQK
                     IPMRKAALALGVERVQKAKRIRGLFP"
     gene            complement(4554..4931)
                     /locus_tag="PKI07_09025"
     CDS             complement(4554..4931)
                     /locus_tag="PKI07_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOC51233.1"
                     /translation="MPPDWEKLHALARAEVEATLAALPAPLRERARALPVTCELRPNA
                     AHRRDGIEPDTLGLFVGPEFADEETSALPLPPQIILFLANLLDLADGDEACFRAEVCT
                     TFLHELGHYLGLDEDDLFDRGLE"
     gene            5359..6612
                     /locus_tag="PKI07_09030"
     CDS             5359..6612
                     /locus_tag="PKI07_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOC51234.1"
                     /translation="MKKHIARVIAAGLVPTLLAAGSAAAGDWPQWRGPNRDGLCPETG
                     LLKEWPPGGPPLLWKAAGLGSGFSGVALAGARLYTLGERPEGNHVLALNRADGKLLWS
                     TRLGKAGAPGWGGFAGPRATPTVDGNLVFALGQYGEVLCVNAADGKQLWSRHLVNDFG
                     GSLPEWGFSESPLVDGEQVLFTPGGARGTLVALNKKTGAVIWQSKEWTDDAHYSSIIL
                     AEIGGVRQYVQLTDKSVAGVAPKDGKVLWRAIRKGATAVIPTPIHADDRVYVTSGYNI
                     GCNLFKVVREGEFTATQVYANKVMVNHHGGVVKVGANLYGYSEGKGWTCQDFATGEAR
                     WQEKNKLGKGSILYADGCLYCREEAEKGAVALLEATPAGYVEKSRFTPPDRSGKKSWP
                     HPVIADGRLYLRDQDVLLCYNVKAR"
     gene            6614..7987
                     /locus_tag="PKI07_09035"
     CDS             6614..7987
                     /locus_tag="PKI07_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51235.1"
                     /translation="MSSANTVSSSRPHYPPGFRARRGLNWSFLGLLYTSFYMCRYNLS
                     IANKSISGEFGFNRAEMGYIITTMLFAYACGQIVNGLLTDRLGGKRAMLIGAAGTILM
                     NILFGVASFAGVLGLFIAIRGVDGYLQAFGAPGMVKINTAWFNHRERGKFSGIFGFMI
                     NLGRLGIFTMGPALLAGFTVLGLINIPPLHWRWLFWAPSIICAVAAVAMALTVKETPE
                     EAGFHGVVRDDTGPADAGVTAQFSQVLRAILTNPAVWITACAYACTGAVRQAVDQWFP
                     RYMQDLYHVDLRSARFQFLAFLIPFVASTGSLVAGYVSDTLFKGRRAPVAAILYFLET
                     AIILLAAQFHNVNAAIVFLVLISFTANSTHSILGSAAPMDIGGRRFAGFALGLIDSFQ
                     YFGGSLAGYFLGNLLDRNLGNYFYFMAPFGAAGGLLMLTIRGRISHAPKPSPAAPAGD
                     AGPPPRK"
     gene            complement(8006..8560)
                     /locus_tag="PKI07_09040"
     CDS             complement(8006..8560)
                     /locus_tag="PKI07_09040"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOC51236.1"
                     /translation="MPTSSKIMMAGCELPIPTPGGEFRARYSARGLCGLAFPAAGGRP
                     GRKAGAVRVPAQVRRWHAVTVRALRRALAGRPAGALPPLDLSAGTAFQQRVWGALRRI
                     GRGQTRSYGQVARAIGQPNAARAVGTACGANPIPVFVPCHRVLAADRRLGGFSGGLDW
                     KRALLRREGVQWRGDGQNPDAGRP"
     gene            complement(8636..9172)
                     /locus_tag="PKI07_09045"
     CDS             complement(8636..9172)
                     /locus_tag="PKI07_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51237.1"
                     /translation="MAWLRKKPDPISDRARALNGEIARLEAQIKELDADLRRAQTQPR
                     LGTTALPGGGPAGRPAAPAAAPSPAPAAEAPIFEEVSQDLLNPRHEAAATPDYYNELG
                     VRKYDLLSLLRRIREHFRGPSTTNPKLVSYLAAGGIQGLRPLRYEKRVARNRFVFFAV
                     ILFLALLGTLLVFLKGQR"
     gene            complement(9185..9862)
                     /locus_tag="PKI07_09050"
     CDS             complement(9185..9862)
                     /locus_tag="PKI07_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51238.1"
                     /translation="MPSDYDATEFVDRDFQAQKLAHAALSGGAGPPLRAPTREEVAAE
                     LARKQQELAAIIQEKEAKEREKAALEETRRRQAEFHTGREEMIQSLTRGLALLEEAEF
                     SARRDAEQMARTLADLRDALAKVQAVREEAWTQDNFSIELTRALTAVENARMEWNAAR
                     LKFPVLSGPAADPSAPAPGALAPASAPLAAHSFGELCRVGLALTWPLAVVALLALAAL
                     LIALLRH"
     gene            complement(9904..10887)
                     /locus_tag="PKI07_09055"
     CDS             complement(9904..10887)
                     /locus_tag="PKI07_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOC51239.1"
                     /translation="MKNTLETRLGIFVALAVLAFAIILERVGTLDSFQRGKRVNALFS
                     NVQDLKAGDLVRMAGVEIGRVQTIQLTNDRVLVRMKVTPSAAVRTDSTATIKFTGLMG
                     QNYVQIDFGTPTGELLKDDGDISTVEQADLSTMMQKIDKVAEGVENMTKSFTGFHIDT
                     LVGPLRDFITANKDPLTLTISNLQMVSSDVATQVRQGQGTLGKLIKDEALYNSALATV
                     SNLQETATEIKTTVAEARKVVDQINSGQGTVGKLVKDEHIYQEAAEAMTQLKEILQKV
                     NQGQGTVGKLINDPELYRNAKLTLQKVDQATEGLEDTGPLSVLGLAVGKLF"
     gene            complement(10919..11677)
                     /locus_tag="PKI07_09060"
     CDS             complement(10919..11677)
                     /locus_tag="PKI07_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC51240.1"
                     /translation="MMNAPASQSNGVSLRVRGLRKAFNGQEVLRGLDFEVAPGEIFVI
                     MGPSGSGKTVLLKHLIGLEAPDAGEILLEGQPLQAPGVMNRYRMAMVFQSGALLNSLT
                     VGQNVGLYLAEHRLRPPGEIARITSEKLAGVGLAGTEDKMPSELSGGMKKRVAIARAL
                     VIEPQLILYDEPTSELDPLSAVVIAEEIVRLKERIGVTSLVVSHDRDLAFGVADRIAV
                     IHQGQMRAIGTPDEVKRHPDPLVQQFLQAGFKRQ"
     gene            complement(11674..12411)
                     /locus_tag="PKI07_09065"
     CDS             complement(11674..12411)
                     /locus_tag="PKI07_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC51241.1"
                     /translation="MFQNIGELVVLFGRTLAALPLVWRQRQKILDQFFEIGVASLLMV
                     CILSFFIGGVMALQTGPLLVERGLGSYVGAIVGYSIAKELAPVLMAVLIAGRIGSAMT
                     AEIGSMRVYQEIDALRTMNINPVHFLVLPRLTALAVALPMLVTIAIVVGWMGGALVAT
                     ANARIAVSFEAFFHALRSAVDLLDVLNGVFKSFMFALIIGMISCHQGLITRGGPRGIG
                     RSVTKAVVNSIVLIVISDYFLTRILLK"
     gene            complement(12456..14009)
                     /locus_tag="PKI07_09070"
     CDS             complement(12456..14009)
                     /locus_tag="PKI07_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51242.1"
                     /translation="MRRTQELAPAAKVQQRKGIVPAPRLAGWGLALLLGVFSGGARAE
                     WPDAGLLFDEFDLTLAPGKRLEAAGPFFYQEQQESTRLWAVPPVLSYARDPERESREL
                     DFLYPVMTYTRLGEQYRWQFLQLLSFAGGPTQEETARDRFTLFPLYFQQRSSDPNRNY
                     TAMVPFYGRLQHRLFRDEIFFVAFPLYSQTRKRDVVTDNYLVPFFHLRHGEGLRGWQL
                     WPLAGKEHKEVTTRTNDFNEVTLVGGHDHFFALWPLYFSHRDGIGTTNEQRQLASLPA
                     YCRLRSPLRDSTTVIWPFFNYVDDREKKYREWDAPWPLVVFARGEGKTTSRVWPFYSR
                     AQDAKRESVFYLWPIYKYNHLHSPPLDRRRTRIGFFLYSDLTEVNTETGAARRRIHCW
                     PLYTQRRDFNGNHRVQVLALLEPFLPANPGIERNYSPLWSVWRAEKNVRTGARSQSLL
                     WNLYRREVTPEAKKCSLLFGLFQYQSDSTNRHLRVGYLPGKRTKTPAPAQATGAANRR
                     SRDGPGSPD"
     gene            14506..16281
                     /gene="ptsP"
                     /locus_tag="PKI07_09075"
     CDS             14506..16281
                     /gene="ptsP"
                     /locus_tag="PKI07_09075"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOC51243.1"
                     /translation="MPELPNMGEKTFRGIPVSAGVCRGKIVVLDRARPDIVQRQLTDE
                     DLPREIKRLEAALAQTRTQITEIQRKVMAAMGAQEGSIFDAHLLALEDRTLLDEVIRM
                     IQEQRVNAEHAFHTIAERYAASLAAVEDDYLRERAADMRDVAGRVLNNLLGLEPEAEL
                     RYFNEPCIIISHDLTPCNTAQLDRRMVLGFATDVGSKTSHTAIMARSMRIPAVVGLKD
                     ASRQLATGQSVLLDGHNGLLIVSPSDQTLGEYGQFMIKQATWRQRLHDLLAEPSITLD
                     GHRVPLHANIEQAADVEQVTANGAEGVGLFRTEYLFLKRDRLPDEEQQYQSYREAAAI
                     LKPRPVVIRTLDAGGDKFLAHMQMPTELNPFLGWRAIRISLQERDIFRAQLRAILRAS
                     AEGNVQVMYPMISGLDELKQANALLEEYKAELRRERVPFDEHLQVGAMIETPSAAVVA
                     DSLAKRVNFFSIGTNDLIQYSLAVDRMNAKIAHLYEPTHPAIIRLIKTTVDAAHQQGI
                     RVSVCGEMGGDPVLTPLLLGLGVDELSAAPVLVPSVKFLIRRLKMSEARELAAFALNC
                     ESAEAILAGCQELTRQIAPGLFEHL"
     gene            16278..17027
                     /locus_tag="PKI07_09080"
     CDS             16278..17027
                     /locus_tag="PKI07_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOC51244.1"
                     /translation="MKVIILAAGYATRLYPLTLTRPKPLLPVAGQPMIDYVLDNLAPI
                     GGLDRIYVVTNAKFVSHFEQWAADYRAHKANLNFTIVNDRSTDDSNKLGAIGDIHFVL
                     EQQRVNDDLIVVAGDNLFSEKLSGFGQYCRERQAPVLALYDVGDLEQVRKYNAITLDA
                     DGRISFFEEKPQNPTSTLTGIALYYYPRAILPLIRQYIAEGNNPDQPGRLIQWLYPRQ
                     PVYTWKVPGLWFDIGSKETLEEANRIFARKS"
     gene            complement(17102..18277)
                     /locus_tag="PKI07_09085"
     CDS             complement(17102..18277)
                     /locus_tag="PKI07_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOC51245.1"
                     /translation="MNRIQYPAMMWAVSLALGLSVCGCQAPSAPEAASSTQTLCPLDS
                     RLTIAVRQFQKTAVGSWETAPVTPPFAFDELIYSWHLRVAPGEGFRLYLRVRDERGDA
                     SPWLYGGYWGKVRLAASRAEPVFAWGKVAMDQLLLQRKAVSFQFRVVDEGDSPLRQPP
                     ALSVVVTDNSPTPALAKRFAPKRKAARTPQCILDLPLRRQADSRGTPLPDRCQSAALA
                     SAMEYFGKPVGLEDIVPLAFDVEYDYPGIWPRTIGAGIQHGFEGYIDRFRDWDYVRKT
                     VAENKVILCSMKMPPGQYKAPPYPQMTGHIVALNGVTDDGRVVVTDSALGKSGRGYRL
                     QWLREDFERVWMQTKGGVGMVICPPAGAPRKLVHDLPPFPEERLKAATAATPGDGRN"
     gene            19022..>19241
                     /locus_tag="PKI07_09090"
     CDS             19022..>19241
                     /locus_tag="PKI07_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51246.1"
                     /translation="MKILSPPRTGSLGAETYYQSPFGVCARRRTVPRDRPSERKAAAR
                     SYFGISSREWGLKLSEAQRERWNAAALHV"
BASE COUNT         3210 a         6312 c         6527 g         3192 t
ORIGIN      
        1 acgcgccgcc gtatttcctc agccaatgcc gggattcttg tccgccaata tatttcgcaa
       61 gcaggccggc ctcgcctccc ggttcttcgc ggcgaggggg cgcggggatt tgggcggagg
      121 attgcggtat gcaattacgc cctttcaggg ctggattggt ttgggggcgc gctattccca
      181 gggcgttgcc ccgggctatc gcagggcgcc ccgttggggc gcgggggcgg gcgcgggaga
      241 gccggggtgc agtccggcgg ccaggtgggc aaattgttcg agggacacgg tttcggcgcg
      301 gacctggggg gagagatcga gctggcggaa cagggcggcg agacgaggct ggggccaatc
      361 cgctttcaac agtttgaaca tcattttgcg gcgctgggag aaggcccgct tgacgatgcg
      421 gacaaacgca tcccttgact ccggcggcag caacgggcgc gggcggcgca ccaggcaaac
      481 gcaggcggaa tccacgtccg gctccgggaa gaagcaggcg gcgggaatcc ggaaccaggt
      541 ttgcggctgg cagttcagtt ggaccagcag ggtcagcacc ccgtagtcgg gcgagccggg
      601 ctgggccatg agccgccggg cgacttccag ttgcagggtg gcgaccaggc gctgcgggcc
      661 gcggtcgccg gccagttcga cgaggatggg cgaggcgacc gagtagggga ggttggccac
      721 cagtttccac tggcgccagt cgcgcggttc gcggcgcagg aactccagcg cgtcgtccgt
      781 gagaatccgc agcgcgcccg ccgcggggct gccggggggc cagcgttcct ggaggaaccg
      841 cgccagttgg gcgtcttttt cgatggccag gacttcgccg gcccgcgccc ggagccgctc
      901 ggtcagcggg ccgaggcccg ggccaatttc cagcacgcgg tcggcgcggg tcagctcggc
      961 cgccgccacg atccggcgca actggttggc gtcgtgcagg aagttttgcc ccagggactt
     1021 ggtgaggcgg atgccgcgcg cggccaggac gcgccgcatg tcggtcagtt tcatgtccga
     1081 ggggcgggcg gcgtcaggcg gcgcattccc ggcgcagcgc cgccacggcg gtccgcagtt
     1141 ggcgggcgga aatggtcagc ggcggcgtga agctgatgac gttggggggg tcgccttcgg
     1201 gcaggaagat gaacccgcgc cgcaacagcg ttttcaccag ggcgagcgcg cgccgggggg
     1261 ccggggagcc gtcggcgtgg cggatttcga cgccggccag caggccctgg ccgcggacgc
     1321 gcagttgggc gccgcgggcg tcgcgggcga gcgccgcgag cgcggccagc aggacacggc
     1381 ccagccgggc gctgcgccgc ggcaggttca gccgccggat ttcccgaatc tgcgccaggg
     1441 ccatggcgca gccgacggga tggcccagaa aggtgctggt gtggatggct tcgccgtcgg
     1501 acggcggcca cgcggcgtcc atgaggtcgg cgcggccgac gcaggcggag agcgggaagc
     1561 cgccggtcag ggctttgccg aggcaaatca cgtccgggat cgttcgggcg tgttcgcagg
     1621 caaaccattt cccggtgcgg ccgaagccgg tgtagatttc atccagaatc agcagggcgc
     1681 ggtgctggtc gcagagccgg cgcaggaggg gcaggaagcc cggcgggggg aggttgacgc
     1741 cgccgcggac ctggatgggc tcggccagga gggcgccgac gcgcccgtcc cggaactggc
     1801 gccggagctg ggattcgagg tcggccaggg cggcggcggg gcgggcgggc gggaagggaa
     1861 cgaagcgccc gaattggcgc aactgcgagc ggaaaggggc gcggaagtgc gcgcgcgcgg
     1921 tgacgttgag ggcgccgtag cccagcccat gatacgcgcc cgtgaaggca atgacccccg
     1981 gctggccggt ggccagccgg gcggttttga gcgcggcctc gacggcttcg aagccggagt
     2041 tgcagaagag ggtcttgccc gtccgcgccg gcgcgggggg ccgggggcgg agcgtccgcg
     2101 tccagcgctc gaacgtgacg cggctcagct cgcgcgccag ttgggctttg agggcgtggg
     2161 ggtggacatc gcccatcgcg tgcagcaggc gcgtcatctg ccgctgcccg gcccgggtca
     2221 cgcgcgggtt ggcgtggccc gccgcggcca cgccgaaggc ggcggtcagg tccaggtatt
     2281 ttcttccctc ggggtcccag acgtgcgttc cccgggcgcg ttcccagacg atgggccacg
     2341 cgccgtcggg gtcgaggaac gtgacgttgc gcgattcgta ggcgcgcagg cgggcgaggg
     2401 tttggcgcgt ggtcatggga gggtcagacg ccgcgggccg cgggcgccca gatgactttg
     2461 tgcaactgga tttggaagcg caccggcagg gcgtcggcga tgatggcctc ggccagttcc
     2521 cggcgcgaga ggggcgtatg gccggggggc acgggtttca gcgcggggtg ccgctgggcg
     2581 ggggcgaggg gctgggccca ggagaacagc agcgggcagc gcgcggcgag ctggcggcgg
     2641 gcgatgcagg ctttggccca ctggtagtcc tggcgcgagg cgatgacaaa cttgagctgg
     2701 tcggtgggcc ggaggcaggc caggttttcc cagcggttgc gcgcggcttc gccgctgctg
     2761 gggcatttga ggtccacgat gcgccgcacc cgggggtcca cgggggaaat atcgcgcgcg
     2821 ccgttggttt cgagcaggac ggtgaagtgg tcgtcgcaca gggcgcgcag gagtgggagg
     2881 gagtttttct ggagcagcgg ctcgccgccg gtgagctcga ccaggggcag gcgttcggcg
     2941 gcggcggggc ggcgggcgcg gagggcgcgg cgcgcggcgg ggggcgcgaa cgggcgggcc
     3001 aggcggcgga cttcgcggcg gatttccgcc agcgagcgcc gggttccggc ggtgaaggcg
     3061 taggcggtat cgcaatagga gcagcgcagg tcgcaggcgg tgaggcggat aaagatgcac
     3121 ggcaggcccg caaacgtgct ttccccctgc aggctcaggt agatttcgtt gacgaccagc
     3181 gtgtcggaca tcggcgccag cataacaggg gggcgggcgc ggggaaagag gcaagcggcg
     3241 gcgcgttttg gctttgcccc cggcgggcgg aaacggttat gctgcggcgc atggatattt
     3301 atgatcatcc cagtttccgg atggcgtgcc agcagttcga cctggtggcg gaccatctgc
     3361 agatgccgga atccgagcgc gcgcgcgtga aataccccaa gcgggccgtg acggtggcgc
     3421 tgccgattca ccgcgacgac ggctccgtgc aggtgttccc gggctaccgg gtgcagcatc
     3481 acctggccct gggcccgacc aagggcggtt tgcgttttca cccggacgtg acgctggggg
     3541 aagtggcggc gctggcgatg tggatgagct ggaaatgcgc gttgatgggg ctgccgtatg
     3601 gcggcgccaa ggggggcatt gcctgcgatc cggcgcaact cagccaggcc gagctggagc
     3661 ggttgacgcg gcgttacacg caggagatga ttccgttcat tggcccgcaa attgatgtga
     3721 tggcgcccga tttggggacc aatgaacagg tgatggcgtg gatgatggat tcctattccg
     3781 tgcatattgg cgccgccgtg cccagcatcg tgacgggcaa acccgtcgcg ctgggcggct
     3841 cgcaggggcg gcgcgaggcc accggccgcg gcgtgggcta cctcgtcaac cgggccaccg
     3901 acacgatcgg gctggacatc agcaaagcca cggccgcgat ccaggggttt ggcaacgtcg
     3961 gctcggtggc ggcgctttcg ctggcgcgct acggggtgaa ggtggtggcg gtgagcgatg
     4021 tgtttggcgg ggtttacaat gccaacggcc tggacctgtg gggcctggaa aagcatgtgg
     4081 cggcgcagaa gaccgtggtg gggttttccg aggcggaacc catcaccaac gagcaactgc
     4141 tggtgctgcc gtgcagcatc ctggttccgg cggcgctgga gcgccagatc acggcggcga
     4201 acgccggcca gatccagtgc cgcatcatcg ccgaggccgc caatggcccc acgacgccgg
     4261 aggccgacga gattctgaac cagcgcccgg agatttttgt catccccgac attctgtgca
     4321 acgcgggtgg cgtggtggtg tcgtacttcg agtgggtgca ggacttgcag agctttttct
     4381 ggggcgagac ggaggtggtg gacaagctgt tccggctgct ggaaagcgcg tttacccggg
     4441 tggtgtccct gagccgcaag cagaagattc ccatgcgcaa ggccgccctg gcgctcggcg
     4501 tggagcgggt gcagaaggcc aagcggattc gggggctgtt cccctagccg gcgtcactcc
     4561 aggccgcggt cgaacaggtc gtcctcgtcc agcccgaggt agtggcccag ttcgtgcagg
     4621 aaggtggtgc acacctccgc gcggaagcag gcttcgtcgc cgtcggccag gtccaggagg
     4681 ttcgccagga aaagaatgat ctgcggcggg agcggcaggg cggaggtttc ctcgtcggca
     4741 aactccgggc cgacgaacag gccgagggtg tccggctcga tgccgtcgcg gcggtgggcg
     4801 gcgttggggc ggagctcgca ggtgacgggc agcgcccggg cgcgctcgcg gagcggcgcg
     4861 ggcagggcgg ccagtgtggc ttccacttcg gcgcgggcca gggcgtgcag tttttcccag
     4921 tccgggggca tggtcttttt atggcgcgga gttgcgtgtt tttgcccgcg ccgtggcaag
     4981 gccgggaagg ggcgggccgg gccggcggcc gggggcaaag cgcgcatcgc acagcgcgtc
     5041 caggacgcgc atctccggcg tccgttgccg gggcggcgcg ccaacgccgg ccgggtgggg
     5101 gagggcggct ctgcggttca tgggagccag tatgcggaga ttgggcgggt tggccagaat
     5161 tatggcgcaa ccccggggcc ggggcgggcg gatggggggc gttagatttg agttcccccg
     5221 ccggcggggt tggcgatatg atgccgccgt tgcgccaaag gcgaattgtc ggcggcgcgc
     5281 gcggccgggc cggcccgagg gtgctggcgt ccatcggcgc atgggtgaac gggaccctcg
     5341 agagttcgca ttggtcagat gaaaaaacat attgcacggg tgatcgccgc cgggctggtt
     5401 cccaccctcc tggccgccgg gtccgcggcg gcgggagact ggccgcaatg gcgcgggccg
     5461 aaccgcgatg ggctttgccc cgaaaccggg ctgttgaagg agtggccccc gggagggccg
     5521 ccgctgcttt ggaaagccgc cggcctcggc agcggtttct ccggcgtggc gctcgcgggc
     5581 gcgcgcctct acaccctggg ggagcgcccc gagggcaatc acgtcctggc cctcaaccgc
     5641 gcggatggca agctcctgtg gtccacgcgg ctgggcaaag ccggcgcgcc gggctggggc
     5701 gggtttgccg ggccgcgcgc cacccccacc gttgacggca acctggtctt tgcgctgggc
     5761 cagtacggcg aggtgctgtg cgtcaacgcg gcggacggca agcagctctg gagccggcat
     5821 ctcgtcaacg acttcggcgg gtcgcttccg gaatgggggt tttcggaatc tccgctggtg
     5881 gacggcgagc aggttctttt cacgcccggg ggggcgcgcg gcacgctggt ggccctgaat
     5941 aagaagaccg gggccgtgat ctggcaaagc aaggagtgga cggatgacgc ccattactcc
     6001 tcgatcattc tcgccgagat cggcggcgtg cgccaatacg tgcagttgac ggataaaagc
     6061 gtggccggcg tggcgccgaa ggacggcaaa gtgctgtggc gggccatccg caaaggcgcc
     6121 acggcggtga ttcccacccc catccatgcc gatgaccggg tctatgtcac ttccggctat
     6181 aatatcggct gcaacctgtt caaggtggtc cgggagggcg agttcaccgc cacgcaggtc
     6241 tatgccaata aagtcatggt gaaccaccac ggcggcgtgg tcaaagtggg tgcgaatctc
     6301 tatggctact ccgagggcaa ggggtggacc tgccaggatt tcgccaccgg cgaggcccgg
     6361 tggcaggaaa agaacaagct ggggaaaggc tcgatcctgt atgccgatgg ctgcctctac
     6421 tgccgcgagg aggccgaaaa aggcgcggtg gccctcctgg aagccacgcc cgccggctac
     6481 gtggaaaaga gccgctttac gccgcccgac cgcagcggca aaaagagctg gccgcacccg
     6541 gtcatcgcgg atggccggct atacctgcgc gatcaggacg tgttgttgtg ctacaacgta
     6601 aaggcccgtt aagatgagct ccgcgaacac ggtttcctct tcccggccgc actatccccc
     6661 gggcttccgc gcgcgccggg gtctcaactg gagttttctg gggctgttgt acacgtcctt
     6721 ttatatgtgc cggtacaacc tctccatcgc gaataaatcc atcagcgggg agtttggttt
     6781 caaccgggcg gagatgggct acatcatcac gaccatgctc tttgcctacg cctgcggcca
     6841 gatcgtcaac gggctgctga ccgaccggct cggcggcaag cgggcgatgt tgattggcgc
     6901 cgccgggacg atcctgatga acatcctgtt tggcgtggcg tcgttcgcgg gggtgctggg
     6961 cctgttcatc gccattcggg gcgtggacgg ctatctgcag gcttttggcg cgccgggaat
     7021 ggtcaagatc aacacggcct ggttcaatca ccgtgagcgc ggcaagttct ccggcatctt
     7081 cggcttcatg attaatctcg ggcgcctggg catctttaca atgggccccg ccctgctggc
     7141 gggcttcacc gtcctcggcc tgatcaacat tcccccgctc cactggcgct ggctgttttg
     7201 ggctccctcc atcatctgcg ccgtggccgc cgtggccatg gcgttgacgg tgaaggaaac
     7261 gcccgaggag gccggcttcc acggcgtcgt tcgcgacgac accggcccgg ccgacgccgg
     7321 ggtgacggcc cagttttccc aggttctgcg cgccatcctc accaacccgg cggtgtggat
     7381 taccgcctgc gcctatgcct gcaccggcgc ggtccggcag gctgtggacc agtggttccc
     7441 ccgctacatg caggaccttt accatgtgga cctgcgctcg gcgcggttcc aattcctcgc
     7501 gttcctgatt ccgtttgtgg cttcgaccgg ctcgctggtg gccggttatg tttccgacac
     7561 gctgttcaag gggcggcgcg cgcccgtggc tgccatcctc tactttctgg aaacggcgat
     7621 tatcctcctg gcggcgcaat tccacaacgt gaatgccgcc atcgtatttc tggtcctcat
     7681 ctcctttacc gccaactcga cccactcgat cctgggctcg gcggcgccga tggatattgg
     7741 cgggcggcgc tttgcggggt ttgcgctggg actgattgac tccttccaat acttcggcgg
     7801 cagcctggcc ggttatttcc taggcaacct gctcgaccgc aacctgggca actacttcta
     7861 tttcatggcg ccgtttggcg ccgccggcgg gttgctgatg ctcacgattc ggggccggat
     7921 cagccacgcg cccaagccca gccccgcggc tcccgccggg gacgcgggcc cgcctccccg
     7981 caaataaacc ggagcgcggc cggggctacg ggcgcccggc gtcggggttt tggccgtccc
     8041 cgcgccactg gaccccttcg cggcggagca gggcgcgctt ccagtccagg ccgccggaaa
     8101 agccgcccag ccgccggtcg gcggccaaaa cacgatggca gggcacaaac accggaatgg
     8161 ggttggctcc gcacgccgtt cccaccgccc gcgcggcatt cggctggcca atcgcccgcg
     8221 ccacctgtcc gtagctccgg gtttgccccc ggccaatgcg gcgcaatgcg ccccacaccc
     8281 gctgctgaaa ggccgtcccg gcggaaagat caagcggcgg cagcgccccg gccgggcggc
     8341 cggccagcgc ccgccgcagc gcccggacgg tcaccgcgtg ccagcggcgg acttgcgccg
     8401 gcacgcgaac ggcgccggcc ttgcgcccgg gccgcccgcc cgccgccggg aacgccagcc
     8461 cgcacaagcc gcgcgcggaa taacgcgccc ggaactctcc gccgggggtg gggatgggga
     8521 gttcgcaccc ggccatcatg atcttactgg aagttggcat gtgcccgcag gtttgagtga
     8581 aaacggttaa gcccggatcc cccggcccgg ggcgcgcggc ggagcggcgg ggtgatcatc
     8641 gctgcccctt gaggaagacc agcagcgtgc ccagcaaagc caggaacagg atgacggcga
     8701 agaagacgaa gcgattgcgc gccacccgtt tctcataacg cagaggccga agcccctgga
     8761 tgccgccggc ggccaggtag ctcaccagtt tcgggttggt ggtggacggc ccgcggaagt
     8821 gctcgcggat gcgccgcagc agggaaagca ggtcgtattt gcggacgccc agttcattgt
     8881 agtagtccgg cgtcgccgcc gcctcgtggc gcgggtttaa caggtcctgg ctgacctcct
     8941 cgaagatggg agcttcggcc gccggggccg gagacggcgc ggcggcaggg gcggcggggc
     9001 ggccggccgg gccgccgccg ggcagggcgg tcgtgcccag gcgcggctgg gtttgggcgc
     9061 gccgcaggtc agcgtccagc tctttgattt gcgcttccag ccgggcgatc tcgccgttca
     9121 gcgcccgcgc gcggtcggaa atcgggtccg gttttttgcg taaccacgcc atagcggccg
     9181 ccggtcagtg gcgcaggagc gcgatcagca aggccgccag ggcgagcagc gccaccacgg
     9241 ccagcggcca cgtcagggcc agacccaccc ggcaaagctc gccgaaactg tgcgccgcca
     9301 gcggggcgga cgccggcgcc agcgcgcccg gggcgggggc gctggggtcg gccgccgggc
     9361 cggagagcac ggggaacttc agccgcgccg cgttccactc catgcgcgca ttttccaccg
     9421 cggtcagggc gcgtgtgagt tcgatgctga aattatcctg ggtccaggcc tcctcgcgaa
     9481 cggcctgcac cttggccagg gcgtcgcgca agtccgccag cgtccgggcc atctgttcgg
     9541 cgtcccggcg cgcgctgaat tcggcttcct ccagcagggc cagcccgcgc gtcaggctct
     9601 ggatcatttc ctcgcggccg gtgtgaaact ccgcctggcg gcggcgggtt tcctccaagg
     9661 cggccttttc gcgttccttg gcttccttct cctggatgat ggccgccagt tcctgctgct
     9721 tccgcgccag ttccgcggcc acctcttcgc gggtgggcgc gcgcagcggc gggccggccc
     9781 cgcccgacag ggcggcgtgg gccagcttct gggcctgaaa atcgcgatct acaaactccg
     9841 ttgcgtcgta atccgaaggc atggagccag cttaggcggt gcgggggcgg gtgtaaagcg
     9901 ggcttagaac agtttgccga cagccaggcc caggacgctt aacggcccgg tgtcctccag
     9961 cccctcggtg gcctggtcca ctttttgcaa ggtcagcttc gcgttccggt acagctccgg
    10021 gtcgttgata agtttgccca cggtgccctg gccctggttg accttttgca aaatctcctt
    10081 gagctgggtc atggcctcgg cggcctcctg gtagatgtgt tcatccttca ccagcttgcc
    10141 cacggtgccc tggccggagt taatctggtc cacgaccttg cgcgcctcgg ccacggtggt
    10201 tttgatttca gtggccgttt cctgcaggtt gctgaccgtg gccagggcgg agttgtagag
    10261 cgcctcgtcc ttgatgagct tgccgagggt gccctgcccc tggcgcacct gcgtggcgac
    10321 atccgaggaa accatctgca ggttggagat ggtcagcgtc agcgggtcct tgttggcggt
    10381 gatgaaatcc cgcagcggcc ccaccagcgt gtcaatatga aacccggtga agctcttggt
    10441 catgttctcg accccctcgg cgactttatc aatcttctgc atcatggtgc tcagatccgc
    10501 ctgctcgacg gtgctgatgt cgccatcgtc cttcaagagt tcgcccgtcg gcgtgccgaa
    10561 atcgatctgc acgtagttct gccccatcag gccggtgaac ttgatcgtcg ccgtgctgtc
    10621 cgtccgcacc gcggcggacg gggtcacctt catccgcacc aggacccggt cgttcgtgag
    10681 ctggatggtc tgaacgcgcc cgatctcgac gcccgccatc cggaccaggt cgcccgcctt
    10741 gaggtcctgc acgttgctga agagggcgtt gacgcgcttg ccgcgctgga acgaatccag
    10801 ggtgcccact cgttccagga tgatcgcaaa cgcgagcacg gccagggcca caaagatgcc
    10861 caggcgggtt tcgagggtgt tcttcatatt gtctttcctt tcttggtatc ggcggaggtt
    10921 attggcgttt gaagccagct tgcagaaatt gttgcaccag cggatccggg tgccgcttca
    10981 cctcgtccgg cgtgccgatg gcccgcatct ggccctggtg gatgaccgcg atgcggtccg
    11041 ccacgccaaa agcgaggtcc cggtcgtgcg agacaaccag cgaggtgacc ccgattcgct
    11101 ccttgaggcg cacaatctcc tcggcaatga ccacggcgga aagcgggtcc aactcgctgg
    11161 tcggctcatc gtagagaatc aattgcggct cgatgaccag cgcccgggcg atggccacgc
    11221 gcttcttcat gccgccggaa agctcgctgg gcattttatc ctccgtgccg gccaggccca
    11281 cccccgcgag cttttccgag gtgatgcggg cgatttcccc gggcggccgc agccggtgtt
    11341 cggccaggta caggccgacg ttctgcccga ccgtcagcga attcagcagc gcgccggact
    11401 ggaagaccat cgccatgcgg taacgattca taacccccgg cgcctgcagg ggctggcctt
    11461 cgaggaggat ttccccggcg tccggcgctt ccagcccgat caggtgcttg agcaggacgg
    11521 ttttgccgct gccgctgggg cccatgatga cgaaaatctc gcccggcgcc acctcgaaat
    11581 ccaggccgcg cagaacttcc tggccgttga acgccttgcg caacccgcgc acgcgcaggc
    11641 tgacgccgtt ggattgggag gcgggcgcgt tcatcatttc agcaggatgc gcgtcaggaa
    11701 gtagtccgag atgacgatta acacaatcga gttaaccacc gccttggtga ccgagcggcc
    11761 aatcccgcgc gggccgcccc gcgtgatcag cccctggtgg caggatatca tcccgatgat
    11821 cagcgcgaac ataaaactct tgaaaacccc gttgagcacg tccagcaggt caaccgccga
    11881 gcgcagcgcg tggaagaacg cctcgaagga aaccgcgatc cgggcgttgg cggtcgccac
    11941 cagcgcgccc cccatccagc ccaccacgat ggcaatcgtc accagcatcg gcagggcgac
    12001 cgccagcgcg gtcaggcgcg gcagcaccag aaaatgcacc gggttaatgt tcatcgtgcg
    12061 caacgcgtca atctcctggt aaacccgcat ggagccaatc tccgccgtca tggccgagcc
    12121 gatccggccc gcaatcagga cggccatcag caccggggcc agctccttgg cgatggaata
    12181 gcccacaatc gcgccaacgt agctgcccag gccgcgttcg accagcagcg ggccggtctg
    12241 caacgccatc acgccgccga tgaaaaacga caggatgcag accatcaaca ggctggcgac
    12301 gccgatctcg aagaactgat cgagaatctt ctggcgctgg cgccacacca gcggcaaggc
    12361 cgccagcgtg cgcccgaaca ataccaccaa ctcgccaatg ttttgaaaca taccgggtcg
    12421 tcaaaccgtc atatcgtccg ccggctcccc cgggctcaat ccgggcttcc gggaccgtcc
    12481 cgggagcggc ggttcgccgc gcccgttgct tgggccggcg ccggcgtttt cgtccgtttc
    12541 ccgggaaggt aacccacgcg caaatgccgg tttgtgctgt ctgattgata ctggaaaagc
    12601 ccgaacagga gcgagcactt tttggcctcg ggcgtgactt cgcgccggta caggttccac
    12661 agcagcgact ggctgcgggc gcccgtccgg acgttcttct ccgcgcgcca gaccgaccag
    12721 agcggcgagt aattgcgctc gatgcccggg ttcgccggca ggaacggctc gagcagggcc
    12781 agcacctgca cccggtggtt gccgttgaag tcgcgccgct gggtgtagag cggccagcag
    12841 tggatccgcc gccgcgcggc gcccgtttcg gtattcacct ccgtcagatc ggaataaaga
    12901 aagaaaccga tgcgcgtgcg ccggcggtcg agcggcggag agtggagatg gttatacttg
    12961 tagatcggcc agaggtagaa aacgctctcc cgcttcgcgt cctgcgcccg gctgtagaac
    13021 ggccacaccc gcgaagtcgt cttcccttcg ccgcgcgcaa agaccaccag cggccagggc
    13081 gcgtcccact cgcggtattt cttctcccgg tcatccacgt agttgaagaa cggccaaatc
    13141 accgtggtcg aatcccgcag cggcgagcgc agccggcaat aggcgggcag cgacgccagt
    13201 tgccgctgct cgttggtcgt gccaattccg tcccggtggc taaagtacag cggccagagc
    13261 gcgaagaagt ggtcatgccc gcccaccagg gtcacctcgt tgaaatcatt ggttcgagtc
    13321 gtgacctcct tatgctcctt cccggccagc ggccagagtt gccagccgcg caggccctct
    13381 ccgtggcgga ggtgaaagaa cggcaccagg tagttatccg tcaccacgtc ccgcttccgc
    13441 gtctggctat acaacggaaa cgcaacaaag aatatctcat cccggaacag ccggtgttgc
    13501 aggcggccat aaaacggcac catcgccgta tagttccggt tcgggtccga cgaacgctgc
    13561 tggaaataga gcgggaacag ggtgaaccgg tcccgcgcgg tctcctcctg ggtcgggccg
    13621 ccggcgaagc tcagcaactg caggaattgc cagcggtatt gctcgccaag gcgagtatag
    13681 gtcatcaccg ggtagaggaa atccaactcc cgggactccc gctccgggtc gcgcgcgtac
    13741 gaaagcaccg gcggcaccgc ccagaggcgc gtgctctcct gctgctcctg gtaaaagaac
    13801 ggacccgccg cctccaggcg cttgccgggc gccagcgtca gatcaaactc gtcgaacaac
    13861 aggccggcat ccggccattc cgcccgcgcc ccccccgaaa acacgccgag cagcaatgcc
    13921 agcccccagc ccgccaggcg cggcgcgggc actatcccct tgcgctgttg aaccttcgcc
    13981 gccggagcca attcctgagt tcgtcgcatt cgccagcgag gctaaagcag ctagcgcggg
    14041 cgtcaagagt cgctttggcc aacccgccgc cccaacccca atcctctcgc cagacaaaaa
    14101 cccctgtttt tcaacgcacc gcttaccccc aacagcgccg caaaagaaca cgcagccggc
    14161 agagggtgtg ggtagctgcg tgaagccgcg tgccacgcag aggcgccctt ccggccccgg
    14221 tcggccgcat gcccccacgc ggcattcccg cttcccctct gcaaacctct gtatatctcc
    14281 gctattgcaa ctgaggcaat ttacaggaaa gaggaacgaa atacgggctg gaaaggcggg
    14341 atttccggac ccggggcctg tgttgccgcc cgtttggatg gcgccggaaa ccgggtggct
    14401 gcctaatctt cgacccgggg cggccaacgc ctgaaagaag cccatttcgc cctcccgcgc
    14461 aaagaattgc ttcccaatcc cgcccgcctt cagtaagact ccgtcatgcc agagcttccg
    14521 aatatgggag aaaagacctt ccggggcatt ccggtttcag ccggggtttg ccgcgggaaa
    14581 atcgtcgtcc tggatcgcgc ccggccggat atcgttcaac gccaattgac ggacgaagat
    14641 ctgccccggg aaatcaagcg cctggaggcg gcgctggctc aaacgcgcac ccagattacc
    14701 gagatacaac gcaaggtcat ggcggcgatg ggcgcccagg agggcagcat cttcgacgcc
    14761 cacctgctgg cgctggaaga ccgcacgctg ctggacgagg tcatccgcat gattcaggag
    14821 cagcgcgtca acgccgagca cgctttccac acgatcgccg aacgctatgc cgcctccctg
    14881 gcggcggtgg aagacgacta tctgcgcgaa cgcgccgccg atatgcgcga cgtggccggg
    14941 cgggtcctga acaatctgct ggggctggag cccgaggccg aactgcgcta cttcaacgag
    15001 ccgtgcatca tcatcagcca cgatttgacg ccctgcaata cggcgcagtt ggaccggcgc
    15061 atggtgctgg gcttcgccac ggacgtcggc agcaagacct cccataccgc catcatggcg
    15121 cgttcgatgc gcatcccggc ggtggtcggc ctgaaagatg ccagccgcca gttagccacg
    15181 ggccaatcgg tcctgctcga tggccataac ggtttgctca tcgtcagccc ctccgaccag
    15241 accctgggcg aatatgggca attcatgatc aagcaggcca cctggcggca acgcctgcac
    15301 gacctcctgg ccgaaccgtc cattaccctg gacggccacc gcgtcccgct gcacgccaac
    15361 atcgagcaag ccgccgatgt cgagcaggtc accgccaatg gcgccgaggg cgtcggcctg
    15421 ttccgcaccg aatacctctt tctcaaacgc gatcgcctgc ccgacgaaga gcagcaatac
    15481 caatcctacc gggaggcggc cgccatcctg aagccccgcc ccgtggtcat ccgcaccctg
    15541 gacgccgggg gcgacaagtt cctggcccac atgcagatgc cgaccgagct caaccccttc
    15601 ctgggctggc gcgccattcg catttccctg caggagcgcg acatctttcg cgcccagctc
    15661 cgcgccatcc tccgcgccag cgccgagggc aacgtccagg tcatgtaccc catgatctcc
    15721 ggcctcgacg aattgaaaca ggccaacgcg ctgctggaag aatacaaagc cgaactgcgc
    15781 cgggaacggg tgccgttcga tgaacatctc caggtcggcg ccatgattga aactccgtcc
    15841 gccgccgtcg tcgccgattc cctcgccaag cgggtcaact tcttcagcat cggcaccaac
    15901 gacctcatcc aatactccct ggcggtggat cgcatgaacg ccaagatcgc ccatctctac
    15961 gagccgacgc atcccgccat catccggctc atcaagacca ccgtggacgc cgcgcaccag
    16021 cagggaatcc gcgtcagcgt ctgcggcgaa atgggcggcg acccggtgct cacccccctg
    16081 ctgctggggt tgggcgtgga tgaattgagc gccgcgcccg tgctggtgcc gtccgtcaag
    16141 tttctcatcc gccgcctcaa gatgagcgaa gcccgggaac tggccgcctt tgcgctcaac
    16201 tgcgagtccg cggaagccat cctggccgga tgccaggaat tgacccgcca aatcgccccc
    16261 ggtctgttcg agcatttatg aaagtcatca tcctggccgc cggctacgcc acccgccttt
    16321 atcctctcac cctcacccgg cccaagccgc tcctgcccgt ggccggccag cccatgattg
    16381 attacgtgct ggataacctg gcgcccatcg gcggcctgga tcgcatctac gtcgtcacca
    16441 acgccaagtt cgtctcccac ttcgaacaat gggccgcgga ctaccgcgcc cacaaggcca
    16501 acctcaactt caccatcgtc aacgaccgct ccaccgacga ctccaataaa ctcggcgcca
    16561 tcggcgacat ccatttcgtg ctcgaacagc agcgcgtcaa cgacgacctc atcgtcgttg
    16621 ccggcgacaa cctcttcagt gagaagctct cgggatttgg ccaatattgc cgcgaacggc
    16681 aggctcccgt gctggcgctt tatgatgtcg gcgaccttga gcaggtcagg aaatacaacg
    16741 ccatcaccct cgacgccgac gggcgcatca gcttcttcga ggaaaaaccg caaaacccca
    16801 cctccaccct gacgggcatc gcgctctact actatccccg ggccatcctg cctttgatcc
    16861 gccaatacat cgccgagggc aacaaccccg accaacccgg ccggctcatc cagtggcttt
    16921 acccccgcca gcccgtctat acgtggaaag ttcccggcct gtggttcgac atcggctcca
    16981 aagaaacact cgaagaggct aaccgcatct tcgcccgcaa atcctgaccg aggcactggc
    17041 ttgcgccgcc ccggcatccc agggcttaag cgcccggccc cggcaccccc gccgcgcagc
    17101 gtcagttgcg cccgtcgccg ggcgtggcgg cggtagcggc cttcaaccgt tcctccggaa
    17161 acggcggcag gtcatgcacg agcttccggg gcgcgcccgc aggcgggcag atgaccatgc
    17221 caacgccgcc cttggtctgc atccacaccc gctcgaagtc ttcccggagc cattgcaggc
    17281 ggtagccgcg gccgcttttg ccgagggccg aatcagtcac caccacgcgc ccgtcatccg
    17341 tgaccccgtt cagggcgacg atatggccgg tcatctgcgg gtaaggcggg gccttgtatt
    17401 gtccgggcgg catcttcatc gaacagagga tgactttgtt ctccgccacg gttttccgga
    17461 cgtaatccca gtcccggaac cggtcaatat agccctcgaa cccatgctgg atgccagcgc
    17521 cgatcgtgcg cggccagatg cccgggtagt catactcgac atcgaaggcc agcggcacta
    17581 tgtcctccag gccgacgggt ttgccaaagt attccatcgc gctcgccagc gcggcggatt
    17641 ggcagcggtc cggcaggggt gtgccccggg aatccgcctg gcggcgcaac ggcaaatcga
    17701 ggatgcactg gggcgtgcgg gcggctttgc gtttaggggc gaaccgcttt gccaacgcgg
    17761 gcgtggggga attgtccgtg accacgaccg agagagccgg cggttggcgc agcggggaat
    17821 cgccctcgtc caccacgcga aactggaaac tcacggcctt gcgttggagc agcagttggt
    17881 ccatggccac tttgccccac gcgaagaccg gctcagcgcg gctggccgca agccggactt
    17941 ttccccagta cccgccgtag agccagggcg aggcgtcacc gcgctcgtcc cgaacccgca
    18001 ggtagagccg gaaaccctcg ccgggcgcaa cccgcagatg ccacgaataa atcaactcgt
    18061 cgaaagcaaa cggcggcgtc acgggggcgg tttcccagct tcccacggcg gttttctgga
    18121 attgccggac cgcaatggtc aaccgcgaat ccaacgggca aagggtttgg gttgaagacg
    18181 ccgcttcggg agcggagggg gcttggcatc cgcacaccga caagccgagc gcgaggctga
    18241 cggcccacat catggctggg tattggattc ggttcatagg ctttcgtgct gtacgccagt
    18301 agatttgacc ccccgcggcc agacaatgca agcgtaatgg gcccttggcg ctgccggatt
    18361 cgctaatttt gctaatcatg agccggggga gttgaggcgg cgggttgggc ggtggctaag
    18421 cgcttcggcg gggatgtctg gccgtgcagt ggcagccgtt gatccgcatc gcgccgtgcc
    18481 ttccgcatcc acagggcagc gttgtcctta aagccagccg gccgccttga accgacagac
    18541 gcttttattc ctgccccttg aacccaaaaa gcggctgtgg gtttaacaaa gccctttccc
    18601 cctcatctac cgaaccttcg gcgctgataa caaaagaccc tgcggccgca gacaatccgg
    18661 gcgggcatgc agatgttttt ctcagccgcc gggcgaacgg cccccgagcg ccgcgtgagg
    18721 gcacgcggcc tacaggaact ggaagtgcgc cgcggcagcc cccatgccct cgcgcagcac
    18781 cgcactcccg acaacccaca ggccccctcg catcatcccg ccccctgtag gccgcgtgcc
    18841 cccacgcggc gctccgcccc cgcccgccac cgccaacccc aaaaaattgc ccttgcgcga
    18901 agacctcccg cccgccagcc ttcccccaga cccttggggt gggggcggct ggaacttcca
    18961 gcccagggaa cggcaggaag ccgagtcgcc ctccggggcg ataacagaaa ggtaattaat
    19021 tatgaaaata ctatcccctc cgcgcacggg cagtctgggc gcggaaacct actaccagtc
    19081 ccccttcggc gtgtgcgccc ggcggcgcac cgttccccgg gaccgaccct cggagcgcaa
    19141 agcggccgca cggtcgtatt tcgggatctc ctcgcgggag tggggtctca agctcagcga
    19201 agcgcagcgc gagcgctgga acgccgcggc gctgcacgtt c
//
LOCUS       DAOPQG010000070        19125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3500_length_19125_cov_15.603146, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000070 DAOPQG010000000
VERSION     DAOPQG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 19125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19125
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3500_length_19125_cov_15.603146"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..530)
                     /locus_tag="PKI07_09095"
     CDS             complement(327..530)
                     /locus_tag="PKI07_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOC51247.1"
                     /translation="MLKVAPLVQNHLAGILAHWKWGLTHAFMEELNRVFSAVKRKARG
                     YRSTVCLIPMHYVVAGKLRLPHT"
     gene            773..2452
                     /locus_tag="PKI07_09100"
     CDS             773..2452
                     /locus_tag="PKI07_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51248.1"
                     /translation="MLLAVVAIGCKPSTPPAPPAPPPPVETVARVHWLGMDRLLAETN
                     ATFLTNITSLPESKQLAKQTLDRLAVGLLATNSPPGTNPPPAAAEGAPSAPLTGVAAL
                     LRPLLEDLFRQESYLEVRHATNQPGEMVLAIRLDDERSGRWQTNLAVILQSRTGHIPA
                     LGQDGRSWQLPLASHPAAAADDRCLTFGRRQDWVLIGLGPETNLFMAELAALIDRDGQ
                     PFARQPKDFWLYANLDPQRTLRALALDWSLPPGLPKVTVTAIGDRGNVRTAAALNFPQ
                     PLPFNLEPWNIPTNLLHEPIVSFTAIQGLTTWLSALPVWRNLQLGSPPGQLYFWAQSG
                     VPFLSYCAATLPDCSNRVTHVTDLLVNQANAWLTNNGLGRFDRATNYHGVNWADLAIV
                     SPYLKTRTQNGVEFAFAGLESDLYTNRPSPAGLLQQVSSITNLVAYDWELTGPRLEQW
                     LYVGQLLRFALYKAQIPPKSASFAWLSALETRLGNCGTVVTRTGPDQLSFLRNSGIGL
                     NSAELDLLADWLESPQFPRGLHTFLGKPTPLPKRKSGASPRPKTTPPAPKG"
     gene            complement(2556..3203)
                     /gene="lepB"
                     /locus_tag="PKI07_09105"
     CDS             complement(2556..3203)
                     /gene="lepB"
                     /locus_tag="PKI07_09105"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOC51249.1"
                     /translation="MRSLMRRQPCRLLAASALAVLLGTGAGRLLIGSIYVVEGASMAP
                     AYPSGAHLYGAPITTPLERGDVVLLDDGRAGCAIKRVIGLPGETVQLWRGCVFINQQL
                     LVEPYLPKHTYTCPVTQARRGAAFALGDKEYFLLGDNRLQSEDSRVYGPVRRCQIKRR
                     VPPPEGFVGGYFASYTLPAYGTTLIRPLTPAHEVSASRNEAGIVQLAARGECQRD"
     gene            complement(3461..4522)
                     /gene="pheA"
                     /locus_tag="PKI07_09110"
     CDS             complement(3461..4522)
                     /gene="pheA"
                     /locus_tag="PKI07_09110"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOC51250.1"
                     /translation="MNISDHRKAIDQFDAQIVRLLNERTQHVLAIGEIKRKNREEIYA
                     PHRERAVLQRICRKNRGPLANESLRAIYREIMSGALSLEKSLTVAYLGPEATFTHQAA
                     IQRFGASLRYAPQKTIHDVFAEVSRHRADYGVVPVENSTEGAVTHTLDMFVDSDLKIV
                     AQIVLPIQHCLLAECARGKIRKLYAHPQSLGQCRVWVQTHLPRVEIIETSSNARSAEL
                     ASKEKNAAAIAGLLAAERYDVSVLDYDIQDNVANATRFLVLGRQCSPPTGHDRTSLMF
                     SLADRVGALYNALVAFRRYRINMTKIESRPSKRKAWEYIFFVDCEGHLQDRRLSKAVA
                     LLGEHCSYVRVLGSYPDSE"
     gene            complement(4536..5279)
                     /gene="scpB"
                     /locus_tag="PKI07_09115"
     CDS             complement(4536..5279)
                     /gene="scpB"
                     /locus_tag="PKI07_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOC51251.1"
                     /translation="MELKLILEALLFSAQKPLNLKELRETLTAAVEHAEDDDRVRELR
                     KVKESELALALEQLRQDHELAQRSYRLVCVAEAWQFVTQPEYAPWLKALVGRKARPPR
                     LSQPALETLAIVAYRQPLTRAEIEQVRGVNVDGVMQTLLERGLVAQVGRAEVVGRPMT
                     YGTTPLFLEYFGLRSLEDLPAADELRRVVIPKPEGLATADPDLATAPPEQLTLPEAAA
                     EPEGPAGEAPVPETATALPAAEPDNHRPE"
     gene            complement(5286..6080)
                     /locus_tag="PKI07_09120"
     CDS             complement(5286..6080)
                     /locus_tag="PKI07_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOC51252.1"
                     /translation="MADYKVKFEVFEGPLDLLLYLIKKEEVDIYEVNLTRLATQFVEY
                     IETMRLLDLEIAGEFLVMAATLMHIKSRELLPVDQQAQVEEEEEGEDPRWELIRQLVE
                     YKKFKDAAAQLQALEARQEDVFPRLPGRLEFAPETPRRAEASVFDLVNAVSAVLKRFT
                     RPPDPRDIFDDPWTVTEKIEYLLRMTRERARIRFSELFAGAGSRAEVVVTFLALLELI
                     RLRQLSAVQPEPFGEIEIYRVQVPEPLAAPAAPDAAASAAADVSPF"
     gene            6572..6648
                     /locus_tag="PKI07_09125"
     tRNA            6572..6648
                     /locus_tag="PKI07_09125"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6606..6608,aa:Asp,seq:gtc)
     gene            complement(6733..6981)
                     /locus_tag="PKI07_09130"
     CDS             complement(6733..6981)
                     /locus_tag="PKI07_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOC51253.1"
                     /translation="MKCAELLAALNEYVDGTVDPAICEEFEQHMAGCNPCQVVVDNLR
                     QTITLYKGGKPIELPAPFRLRLHAALRQHWKKTHRSKG"
     gene            complement(7087..7662)
                     /locus_tag="PKI07_09135"
     CDS             complement(7087..7662)
                     /locus_tag="PKI07_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC51254.1"
                     /translation="MDAFEELTNRYERRVYSLALRLLRHEQDAEDVTQQTFLNALEGL
                     ENFRGEASFATWLLRIATHAALKVIRKRKGLDTVSLEEATEETDRYDTIPHPEYIADW
                     RQSPEELVHQREVQRLLEAALARLDDRHRPVFLLRDVEGLSVRETAEALGLSEANTKV
                     RLLRARLQLREELTRTLGNPARRVIRGAHNH"
     gene            7861..9003
                     /gene="tgt"
                     /locus_tag="PKI07_09140"
     CDS             7861..9003
                     /gene="tgt"
                     /locus_tag="PKI07_09140"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOC51255.1"
                     /translation="MFELLQTDASSQARRGRLTTPHGVVDTPVFMSVGTQGSVKALDP
                     RELREMGTQIILGNTYHLNLRPGLDIIRAAGGLHRFINWPGPILTDSGGFQVFSLATI
                     RKIQAHGVEFRSHLDGSLLFLGPREAMAIQAALGADIAMAFDDCPPHTSTPRELRAAV
                     ERTLRWAAECRQQPRAPGQMVFGIVQGGANAALREECARALVALEFDGYAIGGVSVGE
                     PEPEMMRAVEMTVPFLPDRQPRYAMGLGTPAQMVELVARGVDMFDCVLPTRVARNGTA
                     FTRRGSLSIKAGACKADFRPIEEGCDCFACQHFTRAYLRHLLNVGEILGLRMLSVHNT
                     RMYLRTMEEVRAAIAAGTFAEYRRAFAASYVPTAKVRLARAAAAER"
     gene            complement(9084..10286)
                     /locus_tag="PKI07_09145"
     CDS             complement(9084..10286)
                     /locus_tag="PKI07_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51256.1"
                     /translation="MRKYLLLAIVLVMAGGSWRPPAAWAAAPAAAAASTNAVPPGVEL
                     ARAISEITGVAISPLLGVSAVGAWKYFHARTPQQRARLPWFAQPWFWIPALVLVAACV
                     FKDTVGAAAPRALKKPLDLADAIEHKISGLVAAGAFVPLVIAVFPSPPDEAFRLGTTG
                     FLAAIDLSWLGNGLLVPVAMLAFFFVFLASNAINILILLSPFAVVDAALKGFRLLVLA
                     TLPATAFINPWLGAGWALLIIVLAYLIAGWSFRLSHFGLVFIWDIVTLRSRRFRPAPA
                     ANRMLLARTINQVPARTYGTLRRDDAGRLRLEYRPWLVLPQRVLPLPEGRYHLARGLF
                     YSQILRAEGRGSTAVLLLPPRYRGHEAELVAIYGLTDARDAGLRAAFRWLKDTLGFKG
                     SSAAAAPA"
     gene            complement(10474..12831)
                     /locus_tag="PKI07_09150"
     CDS             complement(10474..12831)
                     /locus_tag="PKI07_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOC51257.1"
                     /translation="MKTISRLSSGPGRALIGAVSLGLAINSLAAAADRFGFSGREIFP
                     IENQISQLHVADLDGDGLNDIVVANNARAKLTLLYNQTGKTNLASKPGPGGRREINEL
                     PPDARFRLASLASEKRITALAVADLNSDGRPDLAYYGDPAELVVLYNEGSTNWSVPRR
                     WPLDDGQLTQNALATGDLNGDGRTDVALLSENCVYILFQQADQTLGEPQKIPISGAPR
                     SIQIVDVDGDGRSDLLLVEWGDRNPFRFRRQTPDGGLGPEIYFASAPIRSYWADRLDA
                     RRQIQVITIAQYSGRARISEFTRQPAKTLAGSFRQGQFQVWPLSRTDKARRGQLWADI
                     NGDGRPDLLIAEPESGQVSLSLQQPDGSQAVPIIFPTLAGISDLAVADAADGGPPEIF
                     MLSADERQVGVTRLDEKQRLPFPTLIPLPGRPLALATGQLRPGAAPVLAVLAESEAKE
                     GPETQRLLVLRSADGRIRTQKLSASFKANPAVLAFHDADQDGLVDLVALTPYEKVKIL
                     RQVPDRDFEELDVAPPGGARELAQPWLGAADIDGDGRRELLLAQKNFLRTVVLAREAP
                     AAAATNQGAWAFTVREQINGADRNSRLAGAAAIPNGTNVIASLFLLDAERKALTLCER
                     DSAGVWQVVRNVPLPVAEFTRLQPLTLGADRTNAVALLGLNAVACLPLDGQVWELTDL
                     DSYETPIKDGFLTDVVSGDLDQDGRKDLVFLETARNYLDLVLFSAQRKLVPANRWQVF
                     EERTYRGRRGDSREPREAAIADVTGDGKNDLIIIVHDRVIVYPQE"
     gene            complement(12856..13920)
                     /locus_tag="PKI07_09155"
     CDS             complement(12856..13920)
                     /locus_tag="PKI07_09155"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOC51258.1"
                     /translation="MMKTRGYAAHDKHSPLVPFTFERREPGPRDVQIEILYCGICHSD
                     LHQVRDDWGGSLYPMVPGHEIVGRVTRVGAEVKKLKVDDLAGVGCMVDSCRHCPACHE
                     GLEQYCAEGPTWTYNARERGREQLTFGGYSERIVVEERFVVKIPPSLDLKTAAPLLCA
                     GITTYSPLRHWKVGKGQRVGVIGLGGLGHMGVKFARAFGAEVVMITTSPAKARDAARL
                     GAHEVLISRDSAAMAKQAGRFHFLLNTIPNPHDVNPYLALLKRDATMVLVGLLTAFEP
                     SLQGGSLISGRRSLAGSVIGGLPETQEMIDFCAAHKIGSDVEMIPIQSVNEAYERLLK
                     NDVRYRFVIDMASLKEAAQP"
     gene            complement(14163..14582)
                     /locus_tag="PKI07_09160"
     CDS             complement(14163..14582)
                     /locus_tag="PKI07_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOC51259.1"
                     /translation="MRQVLHEIKIPTRGRGLYEFTDTVTAWVAQHGFQSGLVTLLLRH
                     TSASLLIQENADPDVRGDLERFFARLVPDGDPIYTHTLEGDDDMPAHVRAALTAVNLS
                     IPVSRGRLALGTWQGLYLWEHRRAPHARTIAAHFIGD"
     gene            complement(14653..>16031)
                     /locus_tag="PKI07_09165"
     CDS             complement(14653..>16031)
                     /locus_tag="PKI07_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51260.1"
                     /translation="ELETARQALEEANRKLAEQTRLAETLTSEKAALQSKLDSLAAQT
                     VNTAELETARQALEEANRKLAEQTRLAEALTGEKESLQARVTALRTDAEAAAALRAEN
                     QLLKKQVADLQAAPAPASPAEELNRQLAQAQAQIAALQSDTEILRLEKSALADRVKQL
                     SDAPAATAVTTRAADAARIKELERERDDLRRSLAVANKELYSRKGRAAAARLAGLEQQ
                     LATLRARLQVYEARQVPYTAEELALFRRPEPRLEQPVKRASRASAQELPAGTVALVAE
                     AQRLHANREYAKAEEKYLQVLQQDRNNAPTLANLAAIELDQNHLAAAETNIMQAIALA
                     PEDAFSQFVLGRLRFVQANYDEALDALSRAARLDPQDAQIQNFLGLTLSEKGLRGPAE
                     TALRKAIQLDPGYGNAHHNLAVIYLTQQPPLAELARWHYQKALAAGHSRNANLEKMIE
                     ARSAAPAP"
     assembly_gap    16032..16131
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16132..16846)
                     /locus_tag="PKI07_09170"
     CDS             complement(<16132..16846)
                     /locus_tag="PKI07_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51261.1"
                     /translation="MKRFSVILALLVAILGARAEGPDDQYIGIYNLIQEADKLNNGGQ
                     PREALPKYLQAQSALQRFQKGYPDWNTRIVSFRLNYLSDKISALSAWAPPAASAAPPG
                     ASPAASPAAPSDWEHQLRALQDQVSRLQADKTVLEAKLKEALSVQPAAMDPRELARAE
                     AQISDLQKENDLLKVSLAQEKNKPVAAPDTQALDEARQSLEEANRKLAEQARLAEALT
                     SEKAALQSKLDSLAAQTVNT"
     gene            complement(16926..17993)
                     /locus_tag="PKI07_09175"
     CDS             complement(16926..17993)
                     /locus_tag="PKI07_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51262.1"
                     /translation="MLGWLLPGGVTVVVLGCAGVLRPFLFWTISYASQYAAAIPIVRG
                     PDVLRASLRAVASPNLLLWILPWFGVLVMWWERRLDEESARAADRRPGAAARGPASAP
                     PPAQDRSRIAHPRSFLMLWLFCSAASASVGFYFREHYFILVLPALAWLSGVAVSRGLY
                     LLKHDRTIELFLAIPMLGLFTLGLGAALIGHGAVWFTLPVDQAMRSIFGSTLFAETAR
                     VADYLKTHAPSDARIAVLGSEPEIYFQARRRAATGHIYMYPLTETTPYALKLQEDLIA
                     EIERVRPAYVVYVDNPFSWLARPDSPQRIFEWWRDYWAANLDLVLTVPVEEGLARGSD
                     MNQPAPGTATGNHILVFKRRP"
     gene            complement(17963..18823)
                     /locus_tag="PKI07_09180"
     CDS             complement(17963..18823)
                     /locus_tag="PKI07_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51263.1"
                     /translation="MSTTSQPAAHPWRRKVRRHRAWLLVGLALLLVAGVRMRLCELPL
                     ERDEGEYAYAGQLLLQGIAPYREVYAMKLPGTYAAYALMMGGFGQSATGIHLGLALVN
                     LASIVMVFLVGRKLMGEAAGVAAAVVFALLSLSPSVLGLSAHATHFVMLAALGGTLAL
                     LRTGREEGGGDAANGPGSSAGERSRAPALRRSAFAPRPFVAGLLFGLAFLMKQHGAFF
                     GLFGALYLLWVRGSQWRAAARVTAGMRAGGVRARRTGLISAARRRAPAGTGSRGTWGC
                     MCWAGCCRAG"
BASE COUNT         3193 a         6236 c         6236 g         3360 t
ORIGIN      
        1 agcccccttt gagcctcctt tgtacacccg ttgtacacag cctaatggga gcgttaggct
       61 ggctggggcg ggtggggcgg gtttgggcgc gggcctgagc cggaaaggag gaatggacga
      121 gtcgcggatg atgtaaaagg acgtttcaag gcgggaggga gtggacgaga acgttcggca
      181 tgtccggagc ggacgaccgg gtgttatcgc gcgccttggt ttttcggcga gatggggcca
      241 aggttggcga agacatttcc cctcccgggc ggaagttgcc aaaggcggct gctcgcgtca
      301 ggggttcctc tacgttttga gtggaatcaa gtgtgaggca agcggagttt gccggccacc
      361 acgtagtgca tggggatgag gcaaacggtg gagcggtagc cgcgagcctt tcgtttaacg
      421 gcgctaaaca cgcggttgag ctcttccata aaggcgtggg tcaggcccca cttccaatgg
      481 gccaggattc ccgccagatg gttctgcacc aacggcgcca ctttgagcat gggactaaat
      541 atccacgcct ggctgcgtca cgcccctcag cgcacccagc ggcaccgggt cttgattttg
      601 ggcgcggaat gctccgaatg tccgcccgga attggatttg cgcgccggtg gcggctgatg
      661 tataaacctg cgttttatgt ggacaagact gatccgggcg cggctgggcg gcagcgccct
      721 cgtgttggcc ggcgccagat tgacacggcg cgcaactctg ccgttgatat ttttgcttct
      781 ggccgttgtg gctattggct gcaaaccctc cacccctccc gcgccgccgg cgccgccgcc
      841 acccgtcgag acggttgccc gcgttcattg gctgggcatg gaccggcttc tggcggaaac
      901 caacgctacc ttcctgacca atatcaccag cctgccggag agcaaacagc tcgcgaagca
      961 aacgctcgac cggctggcag tcgggctcct ggcgaccaat agcccgccgg ggacgaaccc
     1021 gccgcccgca gccgccgaag gcgccccgtc cgctccgctt accggcgtcg cggcgctgct
     1081 tcgccctttg ctcgaggatt tgtttcggca ggagtcctac ctcgaagtgc gccacgcgac
     1141 taaccagccc ggcgaaatgg tcctggccat tcggctggac gacgagcgca gcggccgctg
     1201 gcagaccaat ctcgccgtca tcctccaatc ccgcaccggc cacatccccg ccctggggca
     1261 ggacggccgg agctggcagt tgccgctggc ctcgcaccct gccgccgccg cggatgaccg
     1321 ctgcctgact ttcggccgca ggcaggactg ggtgctgatt gggctggggc cggagaccaa
     1381 ccttttcatg gccgaactgg cagctttgat tgatcgggac ggccagccgt ttgcccggca
     1441 gcccaaagat ttctggctct atgccaacct ggaccctcag cgcaccctcc gcgcgctggc
     1501 cctggattgg agcttgccgc cggggctgcc caaagtgacg gtgaccgcga ttggcgaccg
     1561 ggggaacgtc cgcaccgccg cggcgctgaa tttcccgcag ccgctgccct tcaacctcga
     1621 gccgtggaac atccccacca acctcctgca tgaacccatt gtcagtttta ccgccattca
     1681 gggcctcacg acctggctgt ccgccctccc ggtctggcgg aacctgcagc tcggctcccc
     1741 gcccggccag ctttatttct gggctcaaag cggcgtgccg ttcctgagct actgcgccgc
     1801 caccctcccc gattgcagca accgcgtcac ccacgtcacc gatcttctcg tcaaccaggc
     1861 caacgcctgg ctgaccaata acggccttgg gcggtttgac cgggccacca actaccacgg
     1921 cgttaactgg gcggatctgg ccattgtttc gccctatctc aaaacccgca cccagaatgg
     1981 cgtcgagttt gccttcgcgg gcctggaatc cgatttatac accaaccgcc cgtctcccgc
     2041 ggggctgctc cagcaagtgt cgagcatcac caacctggtc gcttacgact gggagctgac
     2101 cggcccgcgc ctcgagcagt ggctctacgt gggccaactg ctccgcttcg cgctctataa
     2161 ggcccagatt ccccccaaat ccgccagctt tgcctggctc tccgcgctcg aaacccgcct
     2221 tggcaactgc ggaacggtgg tcacgcgcac cggcccggat cagctttcgt tcctgcgcaa
     2281 ttccggcatc ggcttgaact ccgccgaact ggatctgctg gcggactggc tcgaatcgcc
     2341 gcagttcccc cgcggcttgc ataccttcct gggcaaaccc accccgttgc cgaagagaaa
     2401 atccggcgct tcgcctcgcc ccaaaaccac tcccccggcc ccgaagggct gacgctggcc
     2461 gggacttcgt tgccgggatc aggacggaag gcgtccctca agcgcacgct gtcagggaga
     2521 tggcatcggc ggggtaccgg agcgcgccgc cgggttcagt ctcgctggca ttctccccgc
     2581 gcggccagtt ggacgatccc agcctcgttt cgagatgcgg aaacttcatg cgcgggcgtc
     2641 aaggggcgga taagggtcgt tccgtaggcc ggcagcgtgt aggaggcgaa atagccgccc
     2701 acgaagcctt ccggcggggg gacgcgccgc ttgatttggc atcggcgcac cgggccataa
     2761 acccggctgt cctcactctg gagccggttg tctcccaaca ggaaatactc cttgtctccc
     2821 agcgcgaatg ccgccccgcg ccgcgcctgc gtgacggggc aagtgtaggt gtgctttggt
     2881 aaatacggtt ccaccagcaa ctgctggttg ataaagacgc agccgcgcca aagctgcacg
     2941 gtttctccgg gcaacccgat aacccgcttg atcgcgcagc ccgcccggcc atcgtccagg
     3001 aggacgacgt cgccgcgctc cagcggggtc gtaatgggtg cgccgtagag atgcgccccc
     3061 gacgggtaag ccggcgccat gctcgcgcct tccaccacgt aaatcgagcc gatcagcaac
     3121 cgcccggcgc cggtgcccag cagcaccgcg agggcgctcg ccgccagcaa acggcagggc
     3181 tgacggcgca tcagcgaccg catccatccc ccaaccgatg cccttttgcg ggacgctgtc
     3241 tcgtaatggg tcagcccgcg ccgcccgccc gcccgcagag tttcggcgga gacagccggt
     3301 gacccgggcg gctgaggccg ccagttcagg tgcatggccg cctaagctgc aggtttcctg
     3361 ccattttacg ccttgccccg cttctccctt ctggcggccg gggagaaagg gcgcccggcg
     3421 gtcgggcgga cagggggcct ggggcgggat ttgcggaaga ctactccgaa tccgggtagg
     3481 agccgagcac cctgacgtag ctgcaatgct ctcccaggag cgccaccgcc ttgctcaacc
     3541 ggcggtcctg caagtgcccc tcgcaatcca cgaagaagat gtattcccag gccttgcgct
     3601 tgctcgggcg ggactcgatt ttcgtcatgt tgatgcggta gcgtcggaag gccaccagcg
     3661 cgttgtagag cgcgccgacg cgatcggcca ggctgaacat caggctggtg cggtcatggc
     3721 cggtgggggg gctgcattgc cgtcccagga ccaggaaacg ggtggcgttg gcgacattat
     3781 cctggatgtc atagtccaaa acggagacat cgtagcgttc ggcggccagg aggccggcaa
     3841 tcgcggcggc gttcttttcc ttgctcgcca gctccgccga gcgcgcattg gaggaggtct
     3901 cgatgatctc gacccgcggc agatgggtct gcacccagac gcggcattgc cccagcgact
     3961 gcggatgggc gtacagcttg cggatttttc cgcgcgcgca ctcggccagc aggcaatgct
     4021 gaatgggcag gacaatttgc gccacgatct tcaggtcgct gtccacaaac atgtcaaggg
     4081 tatgcgtcac ggccccctcg gtggagtttt cgaccggcac caccccgtaa tcggcgcgat
     4141 gccggctgac ttcggcgaag acatcgtgga tggtcttctg gggcgcatag cgcaggctgg
     4201 cgccgaagcg ctggatggcc gcctggtgcg taaacgtggc ctccggcccc aggtaagcca
     4261 ccgtcagcga cttctccagc gaaagggcgc cggacatgat ttcccggtaa atggcgcgca
     4321 gggattcgtt agccagcggc ccccggttct tgcggcagat gcgctggagc accgcccgct
     4381 cgcggtgggg cgcgtagatt tcctcccggt ttttgcgttt gatttcgccg atggccagca
     4441 cgtgctgcgt gcgctcgttc agcagccgca caatctgcgc gtcaaactgg tcaatggctt
     4501 tgcgatgatc ggagatattc ataaccggca aagtttcatt ccgggcggtg gttgtccggt
     4561 tcggcggcgg gcagtgcggt ggcggtctcc ggaaccgggg cctcgcccgc cggcccttcc
     4621 ggttccgccg ccgcctccgg cagcgtcagt tgctccggcg gcgccgtcgc caggtccgga
     4681 tccgccgtgg ccagcccttc cggcttcgga atgacgaccc ggcgcaattc atcagcggcg
     4741 ggcaaatcct ccaggctgcg caggccgaag tattcgagaa acagcggggt ggtgccatac
     4801 gtcatcgggc ggcctaccac ttccgcgcgg ccgacctgcg ccaccaaccc gcgctccagc
     4861 agggtctgca tgaccccatc cacgttcacg ccgcgcacct gctcgatttc cgcccgggtc
     4921 agcggctggc gataggccac aatggccagt gtttccagcg ccggttgcga gagccgcggc
     4981 gggcgcgcct tgcggcccac cagggccttg agccaggggg cgtattccgg ctgggtgacg
     5041 aattgccaag cttcggcgac gcagacgagc cgatagctgc gctgggccag ttcgtggtcc
     5101 tggcgcagtt gctcgagcgc gagcgccagt tcgctctcct tcaccttccg gagttcccgg
     5161 acccggtcat cgtcctcggc gtgttcaacc gccgcggtta gcgtctcgcg cagttctttg
     5221 agattcagcg gcttttgcgc cgagaacaga agggcctcca aaatcagttt aagttccatg
     5281 ccaggctaga aaggggaaac atccgccgcc gccgatgccg ccgcgtccgg ggccgccggc
     5341 gcggccagtg gctccggaac ctgaacgcgg tagatttcga tttcgccaaa cggctccggt
     5401 tgcacggcgc taagctgccg gaggcgaatc agctccagca gcgccagaaa ggtcaccacc
     5461 acttccgcgc ggctgcccgc tcccgcgaac aattccgaaa accgtattcg cgcccgttcc
     5521 cgggtcatcc gcagcaaata ctcgatcttc tccgtgaccg tccacgggtc gtcgaagata
     5581 tcccgcgggt ccggcggccg ggtaaatcgc ttgagaacgg cgctgaccgc gttgaccagg
     5641 tcaaaaacag aggcctccgc gcgccggggc gtttcgggcg caaattcaag gcggcccgga
     5701 aggcgcggaa acacgtcctc ctgccgcgct tcgagcgcct ggagctgtgc ggccgcatcc
     5761 ttgaacttct tatactcgac cagttggcgg atcagctccc agcgcgggtc ctccccctcc
     5821 tcctcctctt cgacctgcgc ctgttgatcc accggcagca actcgcggct cttgatgtgc
     5881 atcagggtgg cggccatcac cagaaactca cccgcaatct ccaaatccag caaccgcatc
     5941 gtctcgatat actcgacaaa ctgggtcgcc agccgggtga ggttcacctc gtaaatgtcc
     6001 acctcctcct tttttattaa ataaaggagc aaatccagcg ggccttcgaa gacctcgaac
     6061 tttactttat aatcagccac gttgacccac agcgccaccc ggccctgtga ttcccaaaca
     6121 taggtgccgg cccgcgcctt ggcaacgcca gaagtccgac cggctcaatc tggggtgcca
     6181 atcggttgtc ttcgcaacgc cggctttccg gcccgggcat gctcctgaat ccgcggcgat
     6241 cgttgatctc tgccgccctc actttggcgg gaaaggaacc gctggttcgt aagcggttca
     6301 ggtacggggc gttattgctc tcatacattt ccgacagccg ccgaacctcc ttgccgggcg
     6361 cgtttccggg cgaaacaaaa tttctgcaaa attatctgct ttcgcagttg acacggggct
     6421 ggcggcggta tagcttttag gtgttcgggc aacagtacgt ttcttcagcg gggttcccca
     6481 cccccacctc cttggcgtct gttgcccgaa tattttattt tgttcccctt gacgatccgg
     6541 gcggtatttc gtaatttccc tttcccctgg tgggggcgta gctcaattgg ttagagcgct
     6601 gccctgtcac ggcagaggtt acgggttcga gccccgccgc tcccgccatt ctttttcccg
     6661 gggttggtgg aagagaatag ctgtgtcctg gcgcagcaat ggctggaggc gggatagatt
     6721 gaaggccggg gttcagcctt ttgagcggtg cgtctttttc cagtgctggc ggagcgcggc
     6781 gtgcagccgc aggcggaatg gcgcgggcaa ttcgatgggt ttgccgccct tgtaaagcgt
     6841 gatggtctgg cgcaggttgt ccaccaccac ctggcagggg ttgcatcccg ccatgtgctg
     6901 ctcgaactcc tcgcagatgg ccgggtccac ggtgccgtcc acgtattcgt tcaatgccgc
     6961 caataattcc gcgcacttca tatcaggcca tttgcaggga ctgagccgcc agccggcaaa
     7021 aggttacacc cgggcgcggt tgttttctcg gccgggcgag ggcaatcagg ttctttcgtt
     7081 gccgtttcaa tggttatgcg cgccccgtat cacgcgccgg gcggggttgc ccagggttcg
     7141 cgtcaattct tcacggagct gcagccgggc gcggagcaat cgcaccttgg tgttcgcctc
     7201 gctcaacccg agcgcctcgg cggtttccct cacggacaat ccttcgacgt cccgcaacag
     7261 gaagacgggc cggtgccggt cgtccaaccg ggccagcgcg gcctccagca ggcgctggac
     7321 ttcgcgctga tgcaccagct cttccggcga ctgccgccaa tccgcgatgt attccgggtg
     7381 gggaatggtg tcgtagcggt cggtttcctc ggtggcctcc tccagcgaga cggtgtccag
     7441 gcccttgcgt ttgcggatga ccttgagggc cgcgtgggtg gcgatgcgca ggagccaggt
     7501 ggcaaaggat gcctcgccgc ggaagttttc caggccctca agcgcgttca ggaatgtctg
     7561 ctgcgtcaca tcctcggcgt cctgctcgtg ccggagcagg cgcagcgcca gcgaataaac
     7621 ccgacgttca tagcggttgg tcagctcctc aaaggcatcc atttccccgg cttgggcacg
     7681 cgcgaccagc tcgcggtcgc tggggaccgg agcgggcggg ctcgtccggg ctggagcatt
     7741 ctcgttaatg gcgcttacct gtagcagacc gccgccgccg cggtcaatcc cgcaagccgc
     7801 cccgcggccg gggtcacttt ccggttgccc gatttcacgg ggtagttgaa gctcggcgcc
     7861 atgtttgaat tgctccagac cgatgcctcc tcccaggcgc gccggggccg gctgaccacg
     7921 ccgcatggcg ttgtggatac gccggttttt atgtccgtcg gcacgcaggg cagtgtcaag
     7981 gcccttgatc cccgcgagct gcgcgagatg ggcacgcaaa tcatcctggg caacacctac
     8041 cacctgaacc tccggcccgg cctggacatc atccgcgccg cgggcggttt gcaccggttc
     8101 atcaactggc cggggccgat cctgaccgac agcggcgggt tccaggtgtt cagcctggct
     8161 accatccgca aaatccaggc gcatggggtg gagtttcgat cgcacctgga cggttcgctg
     8221 ctttttctcg ggccgcggga agccatggcc attcaagcgg cgctgggcgc ggatatcgcg
     8281 atggcctttg atgattgccc gccgcacacc agcacgcccc gcgaattgcg cgccgcggtg
     8341 gagcgcaccc tgcgctgggc cgccgaatgc cgccagcagc cgcgcgcgcc gggccagatg
     8401 gtctttggca tcgtgcaggg cggcgccaac gccgcgctgc gcgaggagtg cgcccgcgcc
     8461 ctggtcgcgc ttgagttcga cggctatgcc atcggcggcg tcagcgtggg cgagccggag
     8521 ccggagatga tgcgggccgt cgagatgacc gtgccgttcc tgcccgaccg ccagccgcgc
     8581 tacgcgatgg ggctgggcac gccggcgcaa atggtcgaac tggtcgcgcg cggggtggat
     8641 atgttcgact gcgtcctgcc gacgcgggtg gcgcgcaacg gcaccgcttt cacgcgccgc
     8701 ggcagcctca gcatcaaggc cggagcgtgc aaagcggact tccggcccat tgaggagggc
     8761 tgcgactgtt tcgcctgcca gcattttacg cgggcctacc tgcgccacct gctcaacgtc
     8821 ggcgaaatcc tggggctgcg catgttgagc gtccacaaca cgcgcatgta tttgcggacg
     8881 atggaggaag tgcgggccgc gattgcggca ggcacgttcg ccgaataccg ccgcgccttt
     8941 gcggccagct acgtgccgac ggccaaagtc cgactcgccc gcgccgccgc cgcggagcgc
     9001 taaactgtcc gcacgagttt cgcccccggg ggaaggggga aagcctttgg gctcgctcgg
     9061 acgtccgccc gttcgtctgc ctgctacgcc ggcgcggcgg ccgcagacga gcctttgaac
     9121 cccagcgtat ccttcagcca gcgaaacgcg gcgcgcaggc ccgcgtcccg cgcgtccgtc
     9181 aggccgtaga tagcaaccaa ttccgcttcg tggccgcggt agcgcggggg cagcagcagc
     9241 actgcggtgc tgccccggcc ttccgcccgc aggatctgcg aatagaacag ccctcgggcg
     9301 aggtggtaac ggccttcggg cagcggcagc acgcgttggg gcagcaccag ccagggccgg
     9361 tattccaggc gcaggcgccc ggcgtcgtcc cggcgcagcg tgccgtaagt gcgcgcggga
     9421 acctggttga tcgtgcgggc cagcagcatg cggttggccg ccggcgcggg ccggaagcgg
     9481 cggcttcgca gcgtgacgat gtcccagatg aacaccaggc cgaagtgcga cagccggaac
     9541 gaccagccgg cgatcaagta ggccagaaca atgatcagca gcgcccagcc ggcgcccagc
     9601 cagggattga taaaggccgt tgccgggagg gtcgccagca ccagcagccg gaagcccttg
     9661 agcgcagcat ccaccacggc aaaggggctg agcaggatca ggatgttgat ggcattggag
     9721 gcgaggaaaa cgaagaagaa cgccagcatc gccacgggca ccaacaggcc gttgcccagc
     9781 catgagaggt ctatcgccgc caggaacccg gttgtgccca accggaacgc ctcgtctggc
     9841 ggggagggaa agacggcgat gaccagcggc acaaaagcgc cggcggccac caacccgctg
     9901 atcttatgct cgatggcatc ggccagatcg agcggtttct ttaacgcccg cggcgcggcc
     9961 gcgcccaccg tgtctttaaa gacgcaggcg gccaccagca ccagggcggg aatccagaac
    10021 cacggctgcg caaaccaggg caggcgcgcg cgttgttgcg gtgtccgggc gtggaaatac
    10081 ttccacgcgc ccacggcgct aacgcccagc agcggcgaaa tcgccacgcc ggtgatttcg
    10141 gagattgcgc gcgccaactc cacgccgggc ggcacggcgt tggtggacgc cgctgcagcc
    10201 gcgggagcgg cggcccaagc ggccggtggg cgccaactcc cacccgccat aacgagcaca
    10261 atggccagca aaaggtattt tctcatgccc gggaacctcc gtgggcggcc gcgagcccgg
    10321 agcccgacgc ctgccacacg tttctcaaac gcgccagttt caccgttgaa tgattcatca
    10381 acccccagtg tgccctcgcc gcggaccgct tgcgattaga aaaagacgcc gcgtttgtag
    10441 gcgcgcccgc ccccggtgcc agcgcggggc ggattattcc tgcggataaa cgatgacgcg
    10501 gtcatggacg atgatgatga ggtcgttctt gccgtccccg gtcacgtcag cgatggcagc
    10561 ttcgcgcggt tcgcgggagt cgccgcgccg cccgcggtag gtgcgttcct cgaagacctg
    10621 ccagcgattg gctgggacca gcttgcgctg ggcgctgaac aacaccagat cgaggtagtt
    10681 tctggccgtc tccagaaaga ccaggtcttt gcggccatcc tggtccaggt caccgctgac
    10741 gacgtccgtg aggaagccgt ctttaatggg cgtttcgtag ctgtcaaggt cagtcagctc
    10801 ccaaacctgg ccgtccagcg gcaggcaggc gaccgcattc aagccgagca gggcgacggc
    10861 gttggtccgg tcggcgccga gggtcagggg ctgcaagcgg gtgaattcag cgacgggcag
    10921 cggcacatta cgcaccacct gccagacccc ggcggagtcg cgctcgcaca gggtgagcgc
    10981 cttgcgttcc gcatccaaca ggaagagcga ggcaatgacg ttggtgccgt tcgggatggc
    11041 ggcggcgccg gccaggcgcg aattgcggtc cgcgccattg atttgttccc gaaccgtgaa
    11101 agcccaggcg ccctgattgg tggccgccgc tgccggcgct tcccgcgcca ggacgacggt
    11161 gcggaggaag ttcttctggg cgagcagcaa ttcgcgccgg ccgtcgccat caatgtccgc
    11221 ggcgcccagc catggctggg ccaattcccg cgcgcctccg ggaggggcca cgtccaattc
    11281 ctcgaagtcc cggtccggga cctggcggag aatcttgacc ttctcgtagg gcgtcagagc
    11341 cacgagatca acgagtccgt cctgatcggc gtcgtggaag gccaggacgg cgggattggc
    11401 cttgaaactt gcgctcagct tctgcgtccg gatccggccg tccgcggagc gcagaaccag
    11461 caggcgctgc gtttccgggc cctctttggc ttccgactcg gccagcaccg ccaggacggg
    11521 cgcggcgccg gggcggagtt ggcctgtggc cagcgcgagg ggccggcccg gcagcgggat
    11581 gagcgtcggg aaaggcaacc gttgtttttc gtccaaccgg gtgacgccca cctgtcgttc
    11641 atcggcgctg agcatgaaga tttccggcgg gcccccgtcg gcggcgtccg caaccgccag
    11701 gtcgctgatg cccgccagcg tggggaaaat gatcggaacc gcctgcgagc cgtcgggctg
    11761 ctgcaggctg agggagacct ggccgctttc cggttcggcg atgagcaggt cgggccggcc
    11821 gtcgccattg atatcggccc aaagctggcc gcggcgggct ttgtcggtcc gcgagagcgg
    11881 ccagacctgg aactggccct gccggaaaga gccggcgagc gtcttggcgg gctggcgggt
    11941 aaactcggaa atcctggccc gaccggagta ttgggcaatg gtgattacct ggatctgccg
    12001 cctcgcatca aggcggtccg cccagtagga gcggatggga gccgaggcaa agtagatttc
    12061 cggccccagc ccgccgtcgg gcgtttgccg gcggaagcga aacgggttgc gatcccccca
    12121 ttccaccagc aaaagatcgc tgcggccatc gccatccaca tccacgatct ggatggatcg
    12181 aggcgcgcct gaaatgggaa tcttctgcgg ctcgcccaac gtctgatccg cctgctggaa
    12241 caggatatag acgcagtttt cggataacag ggccacgtcg gtgcggccat cgccgttcag
    12301 gtccccggtg gccagggcgt tctgcgtgag ctggccgtca tccagcggcc agcgccgggg
    12361 gacgctccag ttggtggaac cttcgttata gagcaccacc aactccgcgg ggtcgccgta
    12421 gtaggccaag tcggggcggc cgtcgctgtt gagatcggcg acggccagcg cggtgatgcg
    12481 cttttcagag gccagggacg cgaggcgaaa acgcgcgtcc gggggcaact cgttgatctc
    12541 gcgccgtccg ccaggcccgg gcttcgaagc caggttggtt ttgcccgtct ggttatagag
    12601 cagggtgagc ttggcgcggg cattgttggc cacgacaata tcgttcaacc cgtcgccgtc
    12661 caggtctgcc acatgcagtt ggctgatctg gttttcgatg ggaaagatct cccgcccgct
    12721 aaagccaaag cgatcggccg ccgcagccag gctgttgatc gccaacccca ggctgacggc
    12781 cccaatcaag gcccggcccg gtccgctgct aagccgcgaa atcgtcttca cgcagggcgc
    12841 atgccgcaca gggtttcagg gttgagccgc ctctttcagc gaggccatgt ctattacgaa
    12901 acggtagcgg acatcgtttt tcagcagccg ctcataggcc tcgttgacgg actggatggg
    12961 aatcatctcg acgtcgctgc cgatcttatg cgccgcgcag aagtcaatca tctcctgggt
    13021 ctccggcagg ccgccgatta cggatccggc caggctccgc cggccgctga tcaagctgcc
    13081 gccctggagc gacggctcga atgcggtcag caatccgacc agcaccatcg tggcgtcgcg
    13141 tttcagcagc gccaggtagg ggttgacgtc atgcggattg ggaatggtgt tgagcaggaa
    13201 atgaaagcgg ccagcctgtt tggccatcgc cgccgaatcc cgggagatga ggacctcgtg
    13261 ggcgccaagc cgcgccgcgt ccctggcctt ggccggcgaa gtcgtaatca tcaccacctc
    13321 ggccccgaag gcgcgggcga atttgacccc catgtggccc aggccgccca ggccgatcac
    13381 gccgacccgc tggcccttgc ccaccttcca gtggcgcaac ggcgaatacg tcgtgatgcc
    13441 ggcacagagc agcggcgcgg cggtcttcag atcgaggctg gggggaatct tcaccacgaa
    13501 ccgctcctcc accacaatcc gctccgaata cccgccgaag gtcaactgct cacggccgcg
    13561 ttcccgggcg ttgtaagtcc aggtcgggcc ctcggcgcaa tactgctcca gtccctcatg
    13621 gcaagccgga caatggcggc acgaatccac catgcagccg acccccgcca aatcgtcaac
    13681 cttgagcttt tttacctccg cgccgacgcg ggtgacgcgc cccacaatct catggcccgg
    13741 caccatcggg tagagcgagc cgccccagtc atcgcggact tggtggaggt cgctgtggca
    13801 gatgccgcag taaaggattt caatttgcac atcacgcggg ccgggttccc ggcgttcaaa
    13861 ggtgaacggc accagcggcg aatgcttgtc gtgggcggca tagccgcggg ttttcatcag
    13921 ggtcgtatcc ggcgtattca tggttttttg aacatggtcg tatctggtcg gctcaacagc
    13981 ttatgtgcgc gcgttcccag agtagcctga acctggccgg gaggcaagct ggcctcttcc
    14041 gccgcggcag gggttccggc gggccgccgt gtttcgccgg gctttgaaaa cgacacggcc
    14101 cccctcggcc aatcccaacc accgggcctc cccgggatta tcagcgggcc gtttcagctc
    14161 gtttagtcgc caatgaagtg cgcggcaatc gtgcgggcgt gcggtgcgcg acggtgttcc
    14221 cagaggtaga ggccctgcca ggtgcccagg gccaggcgtc cgcggctgac gggaatgctg
    14281 aggttgaccg ccgtaagggc ggcgcgcacg tgcgcgggca tatcatcgtc gccttccagg
    14341 gtgtgggtgt aaatcggatc accgtccggc accagccggg caaagaatcg ttccaggtcg
    14401 ccgcgcacgt ccgggtcggc gttttcctga atcagcagcg aggcggaagt atggcggagg
    14461 agcagggtga ccaggccgct ctggaaaccg tgctgcgcga cccaggcggt caccgtgtcc
    14521 gtgaactcat aaaggccgcg cccgcgcgtg gggatcttga tttcgtgcag gacctgcctc
    14581 atccggggac agtcccttgt cgccgggcct ggaaagttgg ccgcgggcgg cggttgacgg
    14641 cgcggcgggc gctcatgggg cgggggcggc cgacctggct tcaatcatct tttccaggtt
    14701 cgcgttgcgg gaatggccgg cggcgagggc cttttggtag tgccaccggg ccagttcggc
    14761 cagcggcggc tgctgggtca aatagatcac cgccaggttg tggtgagcgt tgccgtagcc
    14821 ggggtccagt tgaatggctt tgcgcagggc ggtttcagcg gggccgcgca gacccttttc
    14881 actgagggtc aagccgagga agttctggat ttgggcgtcc tggggatcca gcctggccgc
    14941 gcggctcaag gcgtcgaggg cttcatcata gttcgcctgg acgaagcgca accggcccag
    15001 cacgaactgg ctgaacgcat cctccggagc cagggcgatg gcctgcatga tattggtctc
    15061 cgccgcggcc agatgattct gatccagctc gatggcggca aggttggcca gggtgggagc
    15121 gttgtttcgg tcctgctgca gcacctgcaa gtatttttcc tcggccttgg cgtattcacg
    15181 attggcatgg aggcgctggg cttcggccac gagggccacg gtgccggccg gcaattcctg
    15241 ggccgaggcc cggctggcgc gcttgacggg ttgttccaac cggggttccg gccgccggaa
    15301 gagggccagt tcttccgcgg tatagggcac ctggcgcgct tcatagacct ggagccgggc
    15361 gcgcagcgtg gccaactgct gttccaagcc cgccaggcgc gcggcggcag ccctgccttt
    15421 gcggctgtaa agctccttgt tggcaaccgc cagggagcgc cgcaagtcgt cccgctcgcg
    15481 ctccagttcc ttgatgcgcg cggcgtcggc agcccgggtg gtgacggcgg tggcggcggg
    15541 ggcatcagaa agctgcttca cccggtccgc cagggccgac ttctccagcc gcaggatttc
    15601 agtgtccgac tgcaaggcgg caatctgcgc ctgggcctgg gccaactgcc gattaagttc
    15661 ttcagccggg gaagcgggcg cgggggcggc ttgcagatcc gcgacctgtt tcttgagcaa
    15721 ctggttttca gcgcgcagcg cggcagcggc ctcggcatcg gtgcgcaagg ccgtcacgcg
    15781 agcctggagg gattctttct ccccggtcag cgcctccgcc aggcgggtct gttcggcgag
    15841 cttgcggttg gcctcttcga gggcctgccg cgcggtttcc aactcagccg tgttaaccgt
    15901 ttgggcggcc aggctgtcga gtttgctttg caaggcggcc ttttcagacg tcagcgtctc
    15961 ggccaggcga gtctgctcgg cgagcttgcg gttggcctct tcgagggcct gccgcgcggt
    16021 ttccaactca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgtgttaa
    16141 ccgtttgggc ggccaggctg tcgagtttgc tttgcaaggc ggctttttcg gatgtcaacg
    16201 cctcggccaa gcgggcctgt tcggcgagct tgcggttggc ctcttcaagc gactggcggg
    16261 cttcgtccaa tgcctgcgtg tccggggccg caaccggctt gtttttctcc tgggccaggc
    16321 tcaccttgag caggtcattc tctttttgca ggtcgctgat ttgcgcctcc gcccgggcca
    16381 attcgcgcgg gtccatggcc gcgggctgaa cggacagcgc ttctttgagt ttcgcctcga
    16441 gcacggtttt gtcggcttgc aaccggctca cctggtcctg aagcgcgcga agttgatgct
    16501 cccagtcgct cggcgccgcc ggtgaggcgg ccgggcttgc gcccgggggg gcggcagagg
    16561 cggcaggggg cgcccatgcg gacagcgcgg agattttgtc cgacaggtag ttcaggcgga
    16621 agctgacaat ccgggtattc cagtccgggt agcctttttg aaaccgctgc agcgcggatt
    16681 gagcctgcag gtacttgggc agcgcctcgc gcggctggcc gccgttgttc aatttgtcgg
    16741 cctcctggat aagattatag atgccgatgt attgatcatc cggcccctcg gctcgcgcgc
    16801 ccagaatggc cacgagcagg gctaagatca ctgaaaaccg tttcatcagg cggcagggta
    16861 aatcaattct tccgcgtttg gcaacggttt catcgcccgc gcgcggcggg gttcgcccgc
    16921 gcggatcagg gacggcgttt gaatacgagg atgtgattgc cggtggcggt tcccggcgcg
    16981 ggctggttca tatccgaccc gcgcgccaag ccctcctcga ccggcaccgt caggaccagg
    17041 tccaggttcg ccgcccaata atcccgccac cactcgaata tccgttgggg cgaatccggc
    17101 cgggccagcc acgagaaggg gttatccacg taaacaacgt aggcgggccg gacgcgttcg
    17161 atttcagcaa tcaggtcctc ttgcagcttt agcgcgtagg gggtcgtttc cgtcagggga
    17221 tacatgtaga tatgcccggt ggcggcgcgg cggcgcgcct gaaaataaat ctccggctcg
    17281 gaacccagga cggcgatgcg cgcgtcgctc ggagcatggg tcttgaggta atccgccacc
    17341 cgggccgttt ccgcaaagag ggtcgagcca aagatgctgc gcatggcctg atccaccggc
    17401 aacgtgaacc agaccgcgcc atgtccgata agcgccgccc ccaggccaag ggtgaacagg
    17461 cccagcatgg gaatggccag gaacagctcg atcgtccggt cgtgcttgag cagatacaaa
    17521 ccgcggctga ccgccacgcc gctcagccag gccagcgccg gcagcaccag gatgaagtag
    17581 tgctcgcgaa agtagaatcc cacactggcc gaggcagccg agcaaaacag ccacagcatg
    17641 agaaagctgc ggggatgggc aatccgcgag cggtcctggg ccgggggcgg cgcggaagct
    17701 ggcccgcggg cggcggcgcc tggccttcgg tcggcggcgc gcgcgctttc ctcatccagg
    17761 cggcgttccc accacatgac cagcacgcca aaccagggga gtatccagag caacagattg
    17821 ggactggcga ccgcgcgcag gctcgcgcgc agcacgtccg gcccccgcac gatcgggatc
    17881 gccgcggcgt attggctggc ataggaaatt gtccagaaca aaaacggccg gagcaccccg
    17941 gcgcatccca gcacaacgac cgtcaccccg cccggcagca gccagcccag cacatacagc
    18001 cccaggtccc gcgcgagccg gttccggctg gcgcgcgccg ccgggcggcg gaaatcaaac
    18061 ccgtcctgcg cgcgcgaact ccgcccgccc gcataccggc ggtgacccgg gccgccgccc
    18121 gccattggct cccccgcacc cacagaagat acagcgcccc aaacaggccg aagaacgccc
    18181 catgctgctt catcaggaaa gccagcccaa aaagcaagcc ggccacgaac gggcgcgggg
    18241 cgaaagccga acgccggaga gccggagcgc gcgagcgttc gccggcggac gagcccggcc
    18301 cattggcggc gtccccgcca ccctcctccc ggccggtccg caagagcgcc agcgttccgc
    18361 ccagggcggc cagcatgaca aaatgggtcg cgtgcgccga taaccccagc acggacgggc
    18421 ttaacgaaag cagcgcaaag accaccgccg cggcgacccc ggccgcctcc cccatcagct
    18481 tccggcccac cagaaacacc atgacaatgg aagcaaggtt gaccagcgcc aggccgaggt
    18541 ggatgccggt ggcgctttgg ccgaacccgc ccatcatcaa agcgtaggcg gcatacgtcc
    18601 ccggcagctt catggcataa acttcgcgat agggcgcgat gccttgcagc agaagctggc
    18661 ccgcgtaggc gtattcgccc tcgtcacgct ccagcggcag ctcgcagagc cgcatccgga
    18721 cccccgccac cagcagcagc gccaacccca ccagcagcca ggcgcgatgg cgccggacct
    18781 tccggcgcca cgggtgggcg gcaggctggc ttgttgtgga catggagacc gcattccacc
    18841 aaaaactgcc ggcggtgaca attccattcc gccgccgccg gttttcggcc gcagggcctt
    18901 tgatttttca gcgcataagt tgagaaggtt gaggctgcgg gggagacggg attacgcgct
    18961 tcggagagga tgtctggccg tgccgcccag ggcttgggcc attgggaaca gtggaagtaa
    19021 gatcgccctc caaggcgagc aggttttccc gcagacaatc caggcgggcg cgcaggtgtt
    19081 tttctcagcc gccgggcgaa cggcccccga gcgccgcgtg agggc
//
LOCUS       DAOPQG010000071        19059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3512_length_19059_cov_16.780099, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000071 DAOPQG010000000
VERSION     DAOPQG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 19059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19059
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3512_length_19059_cov_16.780099"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1362
                     /locus_tag="PKI07_09185"
     CDS             58..1362
                     /locus_tag="PKI07_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51264.1"
                     /translation="MMTTASNNLASRRDFLKTSALVGGALAVPAFLPKDLYGQEKKDV
                     LRVGLIGCGGRGSGAASQALNADPNVVLTALGDAFEDQVQRSLQGLQKQHPDKVKVTP
                     EKCFSGLNAYQQVIDSGVDVVLLAAPPGFRPAHLKAAVAAGKHIFCEKPMATDAPGVR
                     SVLESVEVAKAKKLSLVAGFCWRYELARREFYQRIHEGAIGDLRAVYATYYAGPVKPM
                     PAPSERPADMGDLEWQLRNWYNFTWLSGDGYVEQACHSVDKVAWAFKDRPPLKAVAVG
                     GRQTPNHQGNIFDHMFVVYEYPEEARAFVGQRQVGNTYTENSDYLMGAAGFGKIQGWA
                     APHLKGREEWRYKGPKSDMYQNEHNELFASIRSGKPINDGEWMTHSTLMGIMGRMAAY
                     TGQEITWEQALNSQENLVPAQLDWKMKLDFPPMAMPGVTKFI"
     gene            1391..2299
                     /locus_tag="PKI07_09190"
     CDS             1391..2299
                     /locus_tag="PKI07_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC51265.1"
                     /translation="MQRREFLKTGAGALALAALAPHVLAEAGNPSAAATVKRPLKRGI
                     MWATVGVKGTVLEKMKAIKEAGFDGVEMMSHMDADEVLRARDATGLLIPSVCGRDHWG
                     KPLTHPDPKVRAEGVEALKQTLRDAKRYGASSVLLVPGVVSKEVAYNEAYERSQAEIR
                     KALPLAQELGVSIAIENVWNHFLLSPLEAARYVDEFNSPAVGWQFDVGNILNYGWPEQ
                     WIRILGPRIKVIHIKEFSRKRRDNEGLWKGFEVKLLEGDNNWPAVMKAVDEIGYRGWA
                     ITEQGGGDSPEGLKDLVVRLDKILAA"
     gene            2307..3116
                     /locus_tag="PKI07_09195"
     CDS             2307..3116
                     /locus_tag="PKI07_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC51266.1"
                     /translation="MKRNLKKGIWYTSVPGGTVLEKFQAAKAAGFDGVEPPSHLNQEE
                     ILRARDETGVAVCSVSCGGPTRDLSHPDAAKRAEAVKSLEQAVREAKRYGAASVLVVP
                     GAVTGQISYAETYQRVQESVRKVVPLAEELGVRLAFENVWNYFLLSPMEAARFVDEFR
                     SPAVRWQFDVGNILNFGWPEHWIRILGPRIQTLHIKEFSRKKRDGEGLWKGFAVEYLE
                     GDNDWPAVMKAVDEIGYTGWATLEPAWVPPDVDPVARLRQISEKLDRIIAI"
     gene            3503..4426
                     /locus_tag="PKI07_09200"
     CDS             3503..4426
                     /locus_tag="PKI07_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51267.1"
                     /translation="MLSATVLGGAGMGALYGGPVFVPNGSFEAPLAPPPYVAPYVELQ
                     IDAWQKTPKVFWWDETAYGPWDQLIGIFANTPPGDPSRLENCDGSQALYLFANPGVGV
                     FQDYDSIGSSTNAPSHAFDARFEVGQSYRLTAGVTISYYQPPTNGATLELSLYYRDAA
                     SNQVTVAATSITNAPGVFDAVTNLVDFQVNVPTVAAGDAWAGRHIGIAILATGELSMA
                     GGVWDVDNVRLAATQAPMLTGTAVSNGQVSFTLLSEPGRVFEILATTNPAQALSLWAS
                     LGLVTNHTGSVSFSEPAAHFPGRFYRARQLP"
     gene            4440..5225
                     /locus_tag="PKI07_09205"
     CDS             4440..5225
                     /locus_tag="PKI07_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51268.1"
                     /translation="MRASPAGPGGFTLIELLVVMAIIALLAALLLPALTTAQQASRKA
                     ACLSNLRQVGIAVASYAHDNDGRIPYGPPAPPFTNPSDLYPTTGAPTSLLSLQSGAPV
                     GLGLLLDNHLAAQPKVLFCPGTDQPVDAEAELARVGRYQAQGSYYYRHGGNTQMFSGP
                     ARTNAPPNLRLDNLGRNREGRWIRALAMDSLFLCPPSLEMFNVKPRTHHRLRFADVLH
                     ADGSAVSRSNRDGRYTVDLHSYAELHDAFNRILGVLEKADAQY"
     gene            complement(5226..8018)
                     /gene="glnD"
                     /locus_tag="PKI07_09210"
     CDS             complement(5226..8018)
                     /gene="glnD"
                     /locus_tag="PKI07_09210"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOC51269.1"
                     /translation="MQELLQKIVASAAARLASAPGQSPAEEVARCKAFLKVETHRLKL
                     RHRAGAGGLEICRVRAAILDALLRHLWDTALAARPKLAREKVPPLALVALGGYGRAEL
                     NPHSDIDFMFLHTRQLAEGNRPPPHLSRLIDGVLYPLWDIGLKIGHSVRSLEDCVAVA
                     NTDMHSKTALIEARLVAGDEALFKRFQKTLISRCVAGHESEYLAQRVADQAARRARFG
                     NSASMQEPNLKSGCGGLRDYQNLLWMAFFKYRTRSLKELQAHDLISAAERKQLEAAYD
                     FLLRVRTEMHYHANRALDVLGKNLQPAVAYHLGYRERSPSKRIEEFMREVYIHARGIL
                     LITRTLEQRLALLPPPPRRPGFLPVGRLASLLPAGRKPAPKPVDGFQFLDGEIHPTSS
                     RVFRDHPRRLLRVFLYAQQRGLRLHPDLAQLIRNQLSLVDRAFLADQHARETFLAILG
                     QRGSVAPILRAMHEVDLLGKYLPEFGKLTCLVQHEFYHQYTADEHTLMCLEQLDRVWE
                     ASAPPHAAYAPIFQKLERPYLLYLALLLHDVGKADAHGNHAAASCALAARAARRLQLD
                     DEAAQTLLLVIEHHLLMASVSQRRDLDDPAVIRQFADKVQNPENLALLTLHTFVDAQA
                     TSDKLWNGFKDLLLNSLHHKALQFLTGGDDYLRAEKKRRANLLLEVRRRAPARLSEEE
                     LQAHFASLPERYFQIHSPRQVVADLELAHRFLRLQVSEEDHALAPVVHWHNEPDRGYN
                     VVKVCTWDRAGLFCKIAGSLSAAGLNILSAQIFTRADDIVLDTFYVADAVTGNLADLD
                     QRDRFASVLHRALTGEEVDFRALIGRQQITRPLYQAYLGERMATQIHLDNEASENRTL
                     IEIETEDRIGLLYTISQALTELRLDISAAKICTERGAALDSFYVRELSGGKVLAPERH
                     RAIEHHLRHAIQALDL"
     gene            8347..8955
                     /locus_tag="PKI07_09215"
     CDS             8347..8955
                     /locus_tag="PKI07_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC51270.1"
                     /translation="MSAERDPDAALMLRVKQGDMQAFADLVDKYKQAVMNLANRMLRD
                     ATEAEDLSQMVFVQVFKSAHRYKVSSKFSTWLFTIARNLCLNEIRRRSRHPTDSLEAR
                     SSEPGEPPAPQFEDKAVVSPPDRLLHGELEAKIQQALAELPEKQRLAILLCRQEDFSY
                     EDIARVLGSSVSATKSLIYRGRETLKQKLKPYLESGAWKDAG"
     gene            9003..9497
                     /locus_tag="PKI07_09220"
     CDS             9003..9497
                     /locus_tag="PKI07_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51271.1"
                     /translation="MMNDPICHRLRELMWRRKLTAAEEAELRAWLETHPAAREDWEAE
                     AGLAAALERLPDTAVPNNFTARVLQAVEREAAAARRRTGGGLWRRLGWLPRAAVATGV
                     LGVGLIACHQMQSVERKKLAESVIAVADIPSLPSPDILQDFDAIRASYPAAAPDEQLL
                     ALLK"
     gene            9566..10516
                     /locus_tag="PKI07_09225"
     CDS             9566..10516
                     /locus_tag="PKI07_09225"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HOC51272.1"
                     /translation="MPASAAAVPEAAPTNSSRAHRPALAVLAPELAPPPPIVRSPISF
                     FRELLAMSATERVQALTNRSPESRAQILAKVREYQSLPPDDRELRLQVTELHWHLLPL
                     MMAPGTNRPGQLAGVPSRLRGLVEDRLREWDKLAPEVKQELLENQATLRYLTDIEGRT
                     EEQRQKILANISPARRALLEQGMARWAAMSPDQRHRTLTRFNQFFELTAQEKEKALKT
                     LSGEERRQIEKTLRAFASLAPEQRAACIRSFAKFAGLSLEERHQFLRNAERWQALSPG
                     ERQVWRDLVRNLPPPLPPALPPLPPGNPLRRPAAVATNGN"
     gene            10600..11376
                     /gene="lgt"
                     /locus_tag="PKI07_09230"
     CDS             10600..11376
                     /gene="lgt"
                     /locus_tag="PKI07_09230"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOC51273.1"
                     /translation="MHPIAFKLGGLAIHWYGVMVALGFLAGLWTASRRALRTGIAAEK
                     ILDLGPWLILGAIVGARGLYVISYWRESFAGQPFGEIFAVWHGGLVYYGGLIGASLAG
                     LLYTRLKKLPLGKVADILAPSIALGHVFGRIGCLLNGCCYGRACTLPWAIQFPPGNPL
                     NAPTYPVHPTEVYESLLNLGLYAALAWLYRRKQFDGQVFAVYLVGYALLRSLVDLFRG
                     DYPAHQYYLGGWATPAHLASLVTLVGGLVLLRVLSRARPK"
     gene            11400..12329
                     /gene="miaA"
                     /locus_tag="PKI07_09235"
     CDS             11400..12329
                     /gene="miaA"
                     /locus_tag="PKI07_09235"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOC51274.1"
                     /translation="MTTESEQHPRPLLLAGPTAAGKSELAILLAERLEGEVVSVDSMQ
                     VYRGLDIGTAKPSPADQRRVPHHLIDVVGLNDAFNAASFVRLARQAVAEIQRRGRVPI
                     LCGGTGLYFKAYLEGLGEAPPADPALRAELETVPLPELLRELAALDPVTYEQIDRCNP
                     RRVIRAVEVIRLTGKPFSAQRAEWRPPTPSPPPPTLCLGLTRPAADLHSRIAARVEAM
                     FNRGWVAETEQLLQRGLAENQTALQALGYRQIIEHLRGERSLSETKTLIQLRTRQFAK
                     RQMTWFRNQLSLTWIDLGAEEAAAAVAMILKQL"
     gene            13275..14108
                     /locus_tag="PKI07_09240"
     CDS             13275..14108
                     /locus_tag="PKI07_09240"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HOC51275.1"
                     /translation="MKRREFLKASIAVPALAGLSCASTQNQGGTAARSGGAREYYELR
                     EYHLKSEAERGLVDGYLSKALIPSLNRRGCEPVGVFAQQERPGAPAPGEVNDPRSVLV
                     LIPYSALSAFPSVLAGLDADAEYLAAGAEYLQSPLTRPAFQRIDSWLLQAFAGMPKVE
                     LPPYCRARKPRLFELRTYESHSEVKARKKVEMFNRGEIQLMREVGLGPIFYGQGLIGG
                     NLPSLTYLLSAESTDAHEQHWRAFRAHPAWERMKNDPQYADTVSKISHRFLVPAPYSQ
                     I"
     gene            14141..17173
                     /locus_tag="PKI07_09245"
     CDS             14141..17173
                     /locus_tag="PKI07_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOC51276.1"
                     /translation="MMRRGWRLGVAGWLLCAGGAGLAAEANARFAVDVFGTARGLPSS
                     AVLAVAQTRDGYLWAGTLAGLARFDGFEFTVFDENNTPGLSSSQVRRLYEDRRGQLWI
                     GTENGEVAVARDGQLTRVQLGQTEAPGRLMAICEDTSGSVLLYTDSGLLARYLDGKTE
                     VWHAGSGAPSTCRALIAEESGRLWLGTDTTLVALKPIPVVTDEVRPIRLNYLLASQRG
                     GYWRLANGRVQRCQENRVEQDWGAYPWATTLPIVAACEDREGNLVVGTYGDGVYWFDA
                     EGKATRISSEDGLSHNSVLSLTVDREGSLWVGTNGGGLNRVRRKRFEVLEVSRGAVVQ
                     SVCEDDQGGVWVGCNGNRIYHCRANATQQFRLIPDSSRLDVNPDTLLDVKAVFMGLNR
                     GALGGNWTLAGTWGALRPHLFQLEYGRFAPLRLPESLDGRVSAIFQDRAGHLWLGTQG
                     GLLRFDDLKLYTTREGLSADDVRAIAEDPEGDLWIGTEGGGLNRLHDGRFTWFTKTNG
                     LPGNSISALEVDAEGVLWVATSGGLARFFEGKWTRYSKKEGLASNNLGYLLEDGQGSL
                     WIGSNAGLLRLKKDELNAFAGGKRMTISCRVYGEADGLPATECTSGSQPGAVRGRDGT
                     LWFPTIQGLTSLNPARLNLNTNPPPVIIEAVLIDNRLQNADTLRAAAPAAVTVPPGRE
                     TLTIRYASLNLAAPEKGRFKYQLEGYETGWTERPGNVREALYPKLPPGQYLFQVKAGN
                     EDDVWNETGATLAITVLPPFWQKWWFISLTLIGLLGLVVGAVHYASTQRLQRQLAVLR
                     QKEALEKERARIARDIHDQLGASLTQISLLGELVEIDKDQPREVEGHARQIEQTALET
                     TRALDEIVWTVNPSNDTLDGLITYVCKYAQDYLSVAGVKYRLEAPPQLPPIPITPEVR
                     HNLFLAAKEAITNVVRHAQATAAEIRIRLQEGRFSLEIRDNGRGLAHLDAQAAQTRNG
                     LRNMRKRMEDIGGSFSIASAPEGGAVVRLSVPLESNAP"
     gene            17195..17821
                     /locus_tag="PKI07_09250"
     CDS             17195..17821
                     /locus_tag="PKI07_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC51277.1"
                     /translation="MPITVAIVEDNDQLRGTLARVINRAEGFKCVSQYANAEAALAGL
                     PKDNPEVVLMDINLPGLNGVECVRQLKQLAPQIQAVMLTVYEDTDNIFNALAAGATGY
                     LLKRTKSAELLESIREVHRGGSPMTTHIARKVTQSFQKAGPSQQPTENLSQREQEVLD
                     CLSKGFLYKEIADKLGISYETVHTYIRRIYEKLQVRTRTEAVAKFLQR"
     gene            complement(17818..>19059)
                     /locus_tag="PKI07_09255"
     CDS             complement(17818..>19059)
                     /locus_tag="PKI07_09255"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOC51278.1"
                     /translation="FKFSMKASNPKIMIQCYRLLAARLEQEGPDWNYPLHLGVTEAGE
                     GEDGRIKSAIGIGSLLCDGLGDTIRVSLTEDSPREIEVCRDLLAQIPILTVAAEQTAD
                     HGPRTTDHQLRTSAADLPALASSLQPSAFPFGPFSYTRRPTPEIELPDGVKCGAQQTV
                     RVVVTRATWDKVVPKLTPRADVRPEAVYEDLNILEADPRKFVVNCDTQLVTVKDDVPL
                     PAIAAFRLLAAQLKRLGRNNPILLKDCLSLPSPPLEPKVALLRASVVIGSLLADGIGD
                     AILVRGEPGAGQSLRLAYNILQAAGCRSFKTDYVACPSCGRTLFNLQTVTARIKARTE
                     HLKGVKIAIMGCIVNGPGEMADADFGYVGGAPGKINLYVGKTPLKFSIPEAEAVDRLV
                     DLIREHGKWTEPEPTPPGAGN"
BASE COUNT         3422 a         5449 c         6614 g         3574 t
ORIGIN      
        1 cttctttggg gtttactttg gcccgggtct cgggcttaat gatcagccac tgattctatg
       61 atgactacag cgtccaataa tttggcttcg cggcgggact ttctgaaaac ttcggcgttg
      121 gtgggcggcg cgctggcggt gccggcgttt ctgcccaaag acctttatgg gcaggaaaag
      181 aaagacgtgc tgcgggtggg gctgattggt tgcggcggcc gcggttcggg cgcggccagc
      241 caggcgctca atgccgaccc gaatgttgtt ctgacggcgc tgggcgatgc cttcgaggac
      301 caggtgcaac ggagtttgca ggggcttcag aagcagcatc cggacaaggt gaaggtgacc
      361 ccggaaaagt gtttttcggg attgaacgcc tatcagcaag tcattgacag cggggtggat
      421 gtggtgttgc tggccgcccc gcccgggttc cgccccgccc acctcaaggc ggcggtggcg
      481 gcgggcaaac atattttctg cgagaagccg atggcaaccg acgcgccggg cgtgcgttcc
      541 gtgctggaat cggtggaggt ggccaaggcc aaaaaactct cgctggtcgc cgggttctgc
      601 tggcgttatg agctggcgcg gcgggagttt taccagcgga ttcacgaggg ggccattggg
      661 gatttgcggg cggtctatgc cacttactat gcgggcccgg tcaaacccat gcccgcgccg
      721 agcgagcgcc ccgcggacat gggcgacctg gaatggcaac tgcgcaactg gtacaacttc
      781 acctggctgt cgggcgacgg ctatgtcgag caggcctgcc atagcgtgga caaggtggcc
      841 tgggcgttca aggatcgccc gccgctcaaa gcggtggctg tgggcgggcg gcagacgccc
      901 aaccaccagg gcaacatctt tgaccacatg tttgtggtgt atgaatatcc cgaggaagcg
      961 cgggcgtttg tgggccagcg gcaagtgggc aatacgtaca ccgagaattc ggattacctg
     1021 atgggcgccg cgggtttcgg caagattcaa ggctgggcgg cgccgcacct gaagggccgg
     1081 gaggaatggc gctacaaggg gcccaagagc gacatgtatc aaaacgagca caatgaactg
     1141 ttcgcgagca tccgcagcgg caagccgatc aacgacggcg aatggatgac gcacagcacg
     1201 ctgatgggca tcatgggccg catggcggct tacaccggcc aggaaatcac ctgggagcag
     1261 gccttgaatt cgcaggagaa cctggtcccc gcccagctcg attggaagat gaagctggac
     1321 ttcccgccga tggccatgcc cggcgtcacc aaattcatat aaccccccgt gcgattgttt
     1381 attgaagatt atgcaacgtc gtgaattcct caaaaccggc gccggcgccc tggccctggc
     1441 cgcgctcgcc ccgcacgtcc tggcggaagc cggaaacccg tctgccgccg ccaccgtgaa
     1501 acgcccgctc aagcggggca tcatgtgggc cacggttggc gtgaagggca cggtgctgga
     1561 aaaaatgaag gccatcaagg aagccggttt tgacggcgtc gagatgatga gccacatgga
     1621 tgccgacgaa gtgctgcgcg cgcgcgatgc gaccggcctg ctcatcccga gcgtttgcgg
     1681 gcgggatcac tggggcaaac cgcttacgca tcccgacccc aaagttcgcg ccgagggggt
     1741 ggaagccctc aagcaaaccc tgcgcgatgc caagcgctat ggcgcttcgt cggtgttgct
     1801 ggtgccgggc gtggtcagca aggaagtggc ttacaacgag gcgtatgaac gttcgcaggc
     1861 ggaaatccgc aaagcccttc cgctggcgca ggagctgggc gtcagtattg ccatcgagaa
     1921 cgtttggaac catttcctgc tcagcccgct ggaggccgcc cgttatgtgg acgagttcaa
     1981 cagcccggcg gtgggctggc aattcgatgt cgggaatatc ctcaactacg gctggccgga
     2041 gcagtggatt cgcatcctgg ggccgcgcat caaggtgatt catatcaagg aattcagccg
     2101 caagaggcgg gataacgagg gcttgtggaa ggggtttgag gtcaagctgc tggagggcga
     2161 caataattgg cccgcggtca tgaaggcggt ggacgagatc ggctaccgcg gctgggccat
     2221 caccgagcag ggcggcgggg acagcccgga aggcttgaaa gacctcgtcg tgcgcctgga
     2281 caagatattg gccgcctgaa ctgcttatga aacgcaatct gaaaaaggga atctggtaca
     2341 cctccgtgcc ggggggcaca gtcctcgaga aattccaggc cgccaaggcc gccgggttcg
     2401 atggggttga gccgcccagc cacctcaacc aggaggaaat cttgcgcgcg cgcgacgaaa
     2461 ccggggtggc cgtttgcagc gtcagttgcg gcgggccgac gcgcgatctt tcgcatcccg
     2521 acgcggcgaa gcgcgcggaa gcggtcaaga gcctcgagca ggcggtgcgc gaagccaaac
     2581 gctatggcgc ggcctcggtc ctggtggtgc cgggcgcggt gaccgggcag atttcctacg
     2641 ccgagacgta tcagcgcgtg caggaatcgg tccggaaggt ggtgccgctg gccgaggaat
     2701 tgggggtgcg gctggcgttc gagaatgttt ggaattactt cctgttgagc ccgatggaag
     2761 ccgcgcgctt tgtggacgag tttcgcagcc cggcggtgcg ctggcagttc gacgtgggaa
     2821 acattctcaa cttcggctgg ccggagcatt ggatccgcat cctggggccg cgcatccaga
     2881 cgctgcacat caaggagttc agccgcaaga agcgcgacgg ggagggcctc tggaaaggct
     2941 tcgcggtgga gtacctggag ggcgacaatg actggccggc ggtcatgaaa gccgtggacg
     3001 aaattggcta cacgggttgg gccacgctcg agcccgcctg ggtgccgccg gacgtggacc
     3061 cggttgcgcg cctgcgccag atctcggaga aattagaccg cattatcgcg atctgattcc
     3121 cacggcatgg ttgctggccc ccgttctccc cgcgggcgcg gggagcgttc cggggggcgc
     3181 gatgatgatg cttttcccaa cagatcgccg tccgatgacg gaagtttgct tcggcacggt
     3241 gccgcgggcg cgggattggc gagggatgct catcgcttgc ctcaagcggc acttaggata
     3301 tgatcctggt atgagtggca taggtattat aatggcggaa aaggcatcag gagagttgaa
     3361 ttggccgcgg gccgggcatt tcctgcacaa cgggcgatgg ggagctttgc ggagccggcg
     3421 ccaggccacg gccagtgcct gtcccggagg cagcatgccc agcctttgca ggcgcggcgg
     3481 ctttttccgc gccctccaag ttttgctcag cgcaaccgtt ttgggagggg cggggatggg
     3541 cgcgctctat ggggggccgg tttttgttcc caatggatcc tttgaagcgc cgcttgcacc
     3601 gcctccgtac gtcgcgcctt atgtcgagtt gcagattgat gcgtggcaga agactcccaa
     3661 agtattctgg tgggacgaga cggcgtatgg gccgtgggat cagctcatag gaatatttgc
     3721 gaacacgccg ccgggggacc cctcccgcct ggaaaactgc gacgggagcc aggcgttgta
     3781 tctgtttgcc aatccgggcg tgggggtgtt tcaggattac gattcgattg gcagctccac
     3841 caacgccccc agccatgcgt ttgacgcccg cttcgaggtg ggccagtcct atcggctcac
     3901 ggccggcgtc accatcagct actatcaacc gccgacaaac ggggctactt tggagttaag
     3961 cctctactat cgggatgcgg cgagtaatca agtgactgtg gccgccacca gcatcaccaa
     4021 cgctccgggg gtgtttgacg ccgtgacaaa cctcgtggat ttccaggtca acgtgccgac
     4081 ggtggcggcc ggcgatgcct gggcggggcg gcatataggc attgccatcc tggccaccgg
     4141 ggaactgagc atggcgggcg gcgtttggga cgtggataac gtgcgcctgg ccgcgacgca
     4201 ggcgccgatg ctgaccggga ctgcggtgag caatggccag gtctctttca cgctgctgag
     4261 cgagccgggg cgggtgtttg aaatcctggc caccaccaat cccgcgcagg cattatccct
     4321 ctgggcgagt cttggcctcg tgaccaatca caccggcagc gtttcttttt ccgagccggc
     4381 cgcccatttc cccggtcgct tttaccgggc gcggcagttg ccgtaggcgg gacggtttga
     4441 tgcgcgcttc tccggcaggg ccgggcggct tcaccctgat cgaactgttg gtggtgatgg
     4501 cgatcatcgc cctgctggcg gcgctcttgc tgccggcgct gaccacggcg caacaggcga
     4561 gccggaaggc tgcctgcctg tcgaacctgc gccaggtggg aatcgcggtc gccagctacg
     4621 cgcatgacaa cgacgggcgg attccgtatg gccctccggc accgccgttc actaatccgt
     4681 cggacttgta tcccacgacc ggggcgccga cgagcctgtt gtctctgcaa tccggcgccc
     4741 cggtggggtt gggcctgctc ctggataatc acctggccgc gcagcccaag gtgcttttct
     4801 gtccgggcac cgatcaaccg gtggatgcgg aggcggaact ggccagggtg gggcgctacc
     4861 aggcccaggg cagctattac taccgccacg ggggcaatac gcaaatgttc agcggccccg
     4921 cccgcaccaa tgcgcctccc aacctgcgcc tggataacct ggggcggaac cgcgaggggc
     4981 gatggatccg ggccttggcg atggattcgc ttttcctctg cccgcccagc ctggagatgt
     5041 tcaacgtcaa gccccgcacc caccaccggc ttcgctttgc cgacgtcctc catgcggacg
     5101 gcagcgcggt ttcccgctcg aaccgggatg gccgctatac cgtggacttg cacagctacg
     5161 cggagttgca tgatgcgttc aaccggatac tcggggtcct ggaaaaggcg gacgcgcagt
     5221 attgattaaa ggtccagggc ttgaatggcg tggcggagat ggtgctcgat ggcgcggtgg
     5281 cgttcagggg ccaggacctt gccgccggat agctcgcgga cgtagaagct gtcgagggcg
     5341 gcgccgcgtt cggtgcaaat cttggcggcg gagatgtcca ggcgcagctc ggtcagggcc
     5401 tgggaaatgg tgtagagcag gccgatgcga tcttccgttt cgatttcaat gagggtgcgg
     5461 ttttccgagg cctcattatc caggtggatt tgtgtggcca tgcgctcccc gaggtaagcc
     5521 tggtagagcg ggcgggtaat ttgttgccgt ccaatcaggg cgcggaagtc aacctcctcg
     5581 ccggtcagcg cccggtggag gacgctcgca aagcggtcgc gctggtcgag gtcggccagg
     5641 ttgccggtga cggcatcggc gacgtagaag gtgtccagca cgatgtcgtc ggcgcgggtg
     5701 aagatctggg cgctgaggat gttgaggccg gcggcgctga gcgagccggc gattttgcag
     5761 aacagcccgg cccggtccca ggtgcagacc ttgacgacgt tatagccgcg gtccggctcg
     5821 ttgtgccagt ggacgacggg ggccagggcg tgatcctcct cggagacctg gaggcgcagg
     5881 aaacggtgcg cgagttccag gtcggccacg acctgccggg gggagtgaat ctggaaatac
     5941 cgctcgggca gcgaggcaaa atgggcttgc aactcctcct cgctcaggcg ggcgggggcg
     6001 cggcggcgca cctcgagcag gagattcgcg cggcgtttct tttcagcgcg gaggtaatca
     6061 tcgccgccgg tcaggaactg cagggccttg tggtgcagcg agttgaggag caggtccttg
     6121 aagccgttcc agagcttgtc gctggtggcc tgcgcatcca caaaggtgtg gagcgtcagc
     6181 agggccaggt tttccgggtt ctgcaccttg tcggcaaact ggcggattac cgccgggtca
     6241 tccaggtcgc gccgctggga aacgctggcc atcagcaggt gatgttcgat cacgagaagc
     6301 agcgtctggg cggcctcgtc gtcaagctgc aggcgccggg cggcgcgcgc ggccagcgcg
     6361 cagcttgccg ccgcgtggtt gccgtgggca tcggctttgc cgacatcgtg caggagcagc
     6421 gccaggtaga gcaggtaggg gcgctccagc ttctggaaaa tgggggcata ggccgcgtgc
     6481 ggcggcgccg aggcttccca gacgcgatcc aactgttcga ggcacatgag cgtatgctca
     6541 tccgccgtgt attggtgata aaactcgtgc tgaaccaggc aggtgagctt gccgaattcc
     6601 ggcaggtatt tgccgagcag gtccacttca tgcatggcgc gcaaaatcgg ggcgacgctg
     6661 ccgcgctggc cgagaatggc gaggaaggtt tcacgcgcgt gctggtcggc cagaaaagcg
     6721 cggtccacca ggctgagctg gttgcgaatg agctgcgcca ggtcgggatg gaggcgcagc
     6781 ccgcgctgct gcgcgtaaag aaacacgcgc aggagccggc gcgggtggtc gcggaagacc
     6841 cgggaggagg tgggatggat ttccccgtca agaaactgga agccgtccac aggtttgggg
     6901 gcgggtttgc gtccggccgg gagcaagctc gcgagccggc caacgggcag gaaacccggg
     6961 cgtcgcggcg gcgggggcag gagggccagg cgctgctcca gggtgcgcgt gatgagcaga
     7021 atgccgcggg cgtggatata cacctcgcgc atgaactcct cgatgcgctt gctgggcgag
     7081 cgttcgcggt agccgaggtg gtaggccacg gcgggttgca ggtttttccc cagcacatcc
     7141 agcgcgcggt tggcgtggta gtgcatctcg gtgcggacgc gcagcaggaa atcgtaggcg
     7201 gcctccaact gcttgcgttc ggccgcgctg atcaaatcat gcgcttgcag ctccttcagc
     7261 gaccgggtgc ggtatttgaa gaaagccatc cacagcaggt tctggtagtc gcgcaggccg
     7321 ccgcagccgc ttttgaggtt gggctcctgc atggaggcgg agttgccaaa tctggcgcgg
     7381 cgggcggcct ggtcggccac ccgctgggcc aggtactcgg attcgtggcc ggcgacgcac
     7441 cggctgatca gggttttctg gaaccgtttg aacagcgctt cgtcgcccgc tacgagccgg
     7501 gcctcgatga gcgcggtctt ggagtgcatg tcagtgttgg cgacggccac gcagtcttcg
     7561 aggctgcgga cggaatgccc gatcttgagg ccgatgtccc acagcggata cagcaccccg
     7621 tctatgaggc gggacaggtg cggcggcggg cggttgccct ccgcgagctg ccgggtgtgg
     7681 aggaacatga agtcaatatc gctgtgcggg tttagctcgg cccggccgta gccgccaagg
     7741 gcgaccaacg ccagcggcgg gactttttcc cgggccagct tgggccgggc cgccagggcg
     7801 gtgtcccaca ggtggcgcag cagtgcgtca agaatggccg cgcgaacgcg acagatttcg
     7861 aggccgccgg cgccggcccg gtgccgcagc ttcagccggt gcgtttccac cttgagaaag
     7921 gccttgcagc gggccacctc ctcggcgggt gattggcccg gcgctgacgc caggcgcgcg
     7981 gcggcactgg cgacgatttt ctgcaacagt tcctgcattc aacgagcact gtggaaccaa
     8041 acgcgccgca gagcaagcac ctgaaatcag gcagccggga agccggcgtg cccccgtatt
     8101 tgcggatcct ggtttgccgc caaaatgtcc gaaaaaaagc ggtgacgcgg cagcggtgtt
     8161 tccggcgcgg acaggctggc ttggcgaacc gcggcaggcg tcccgaggcc gggatttgtt
     8221 tcgcttcctg cgttagcggc gcccggaacc gcggcgcgca accttgcttt gtgaggcgcg
     8281 acccccggcg aaggcgcgca tgcgggaaac cttgtaagcg cggcggggtt tgttaggctg
     8341 gcgaatatgt ctgctgagag ggatcctgat gccgccctga tgctgcgcgt gaagcagggt
     8401 gacatgcagg cctttgccga tttggtggac aaatataagc aagcggtgat gaacctggcc
     8461 aatcggatgc tccgggatgc cacggaagcc gaggatctca gccagatggt attcgtccag
     8521 gtgttcaaat cggcgcaccg ctacaaggtt tcctccaagt tctcgacctg gctgtttacc
     8581 atcgcgcgga atctgtgcct gaacgaaatc cgccgccgct cccggcaccc gactgactcg
     8641 cttgaggcgc ggtcttcgga gccgggggag ccgccagcgc cgcagttcga agataaggca
     8701 gtagtttcgc cgccggatcg cctgttgcac ggggaactgg aggcaaagat tcaacaggcg
     8761 ctggctgaac tgccggagaa acaacgcctg gccatccttc tctgccgcca agaggacttc
     8821 agctacgagg atattgcccg cgtgcttggg agctcggttt ccgcgaccaa atcgctgatt
     8881 taccgcgggc gggagacgct taagcagaag ctcaagcctt acctggaaag cggcgcatgg
     8941 aaggatgcgg gatgaacgca actttggcgc cggaatggtt taattatgct taacagtgct
     9001 gtatgatgaa cgacccgatc tgccaccggc tgcgcgagct gatgtggcgg cgaaaattga
     9061 ccgccgccga ggaggccgaa ttgcgggcct ggctggagac ccatcccgca gcgcgggagg
     9121 attgggaggc ggaggccggg ctggcggcgg ccctggagcg gctgcccgac acggcggtgc
     9181 cgaacaattt caccgcgcgt gtgctgcagg cggtcgagcg ggaagcggcg gcggcccgcc
     9241 gtcggacggg ggggggacta tggcggcggt tggggtggct gcccagggcc gcggtagcga
     9301 cgggcgtgct gggggtgggg ctgatcgcct gccaccagat gcaaagtgtc gagcgcaaga
     9361 agctggcgga aagcgtgatt gcggtggcgg acattccttc actgcccagt ccggatatct
     9421 tgcaggactt cgacgccatc cgcgcatcgt atcccgcggc cgccccggac gaacaattgc
     9481 ttgccttgct gaaatgaagg gcggccggcg taatggaacc ggagtggcgg ctgcgctgtg
     9541 cgccagcttg ttgtggcggg gggcaatgcc ggcgtcggcg gccgccgtgc cggaggcggc
     9601 ccccaccaat tccagtcggg cgcaccggcc cgcccttgcc gtgctggctc ccgagttggc
     9661 gccaccgccg cccattgtcc ggtcgcccat tagcttcttt cgcgagttgc tggcgatgag
     9721 cgccaccgag cgcgtccagg cgctgaccaa ccggtcgccg gagagccgcg cgcagatatt
     9781 ggcgaaagtg cgcgaatatc agtccctgcc gccggacgac cgcgagctgc gcctgcaggt
     9841 gacggaattg cattggcatc tgctgccgct gatgatggcc ccgggcacca accgccccgg
     9901 gcaactggcg ggcgtgccgt cgcggttgcg ggggctggtg gaagatcggc tgcgcgaatg
     9961 ggacaagctg gcgccggagg taaaacagga actgctggaa aaccaggcca cgctgcgcta
    10021 cctgacggat atcgaagggc gcaccgaaga gcagcggcag aagattttgg cgaatatttc
    10081 acctgcccgt cgtgccctgc ttgagcaggg catggcgcgg tgggcggcga tgtccccgga
    10141 ccagcggcac cgaacgctga cccggttcaa ccagttcttt gagctgacgg cccaggaaaa
    10201 ggagaaagcg ctcaagacgc tttccggcga ggaacggcgg caaatcgaaa agacgttgcg
    10261 ggcctttgcc agcctggcgc cggagcaacg ggcggcgtgc attcgttcgt tcgcgaaatt
    10321 cgccggcctg agtctggagg aacggcacca gtttctccgg aacgcggaac gctggcaggc
    10381 actgtcgccc ggcgaacgcc aggtctggcg cgatctggtt cggaatctgc cgccgccgct
    10441 gccgcccgcc ttgccacccc tgccacccgg aaatcccctc cggcgcccgg cggccgtcgc
    10501 caccaacgga aactaagccc ccccgcaggt tgcgcccggg ccgttgccgg ggcgcaattt
    10561 tattttccag ttggccgact cgcgtctaat ctggaggcga tgcatcccat tgcgtttaaa
    10621 ttgggcgggt tggccatcca ttggtatggg gtgatggttg cgctgggttt tctggcgggt
    10681 ttgtggactg ccagccgccg ggctttgcgc acgggcatcg ccgccgaaaa gattctcgac
    10741 ctcggcccgt ggctcattct gggggcgatt gtgggcgcgc gggggcttta cgtcatttcg
    10801 tattggcgcg agtcgtttgc cggtcagccg tttggggaaa tcttcgccgt ttggcacggc
    10861 ggcctggtct attacggagg gctgataggc gcgtcgctgg ctggcctcct ttacacccgt
    10921 ttgaaaaagc tgccgcttgg gaaggtggcg gacattctgg cgccaagcat tgcgctgggc
    10981 cacgtcttcg ggcgtattgg ctgtctgctg aacggttgct gctacgggcg tgcctgcacg
    11041 ttgccctggg ccattcagtt tccgccgggc aacccgttga atgcgcccac ttacccggtg
    11101 catccgacgg aggtttatga atcgctgctg aacctgggtt tgtatgcggc gctggcctgg
    11161 ctgtatcgcc ggaagcagtt cgacggccag gtcttcgccg tctatctggt gggctatgcg
    11221 ctgctgcgct cgctggtgga cctgttccgc ggtgattatc ccgcgcacca gtattatctg
    11281 ggcggctggg ctacgccggc gcatctggca agtttggtga cccttgtcgg gggcctggtt
    11341 ttgctgcgcg tgctgtcccg cgcccggcca aagtgaggag gcgggagcgg cgagcgccca
    11401 tgaccaccga atccgaacag catccgcgcc cgcttcttct cgcaggcccg acggcggccg
    11461 gcaagtcgga acttgccatc ctgctggcag aacggctgga gggagaggtt gtttcggttg
    11521 attcgatgca ggtttatcgc gggctcgaca tcggcacggc aaaaccctct cccgccgacc
    11581 agcgccgcgt gccccatcac ctgattgatg ttgtggggct gaacgacgct ttcaacgccg
    11641 ccagctttgt ccggctggcg cggcaggcgg tcgcggagat tcagcgtcgc ggccgggtgc
    11701 cgattctttg cggcggaacg ggcttgtatt tcaaggcgta tctggaggga ctgggagagg
    11761 caccgccggc ggatccggcc ttgcgcgcgg aactggaaac cgtgccattg ccggaattat
    11821 tgcgggaact ggcggcgctg gaccccgtga cctacgagca gattgaccgg tgcaatcccc
    11881 gccgggttat ccgtgcggtt gaggtcattc gcctgaccgg caagcccttt tctgcccagc
    11941 gcgcggagtg gcgcccccca acgccttcac cccctcctcc cacactgtgc ctgggcctga
    12001 cgcgcccggc ggcggatttg cattcgcgca ttgcggcgcg ggtggaagcc atgttcaacc
    12061 gcggttgggt ggccgaaacc gaacaactcc tccagcgcgg gttggctgaa aaccaaacgg
    12121 ccctgcaggc gctgggctac cgccagatca tcgagcatct gcgcggggaa cgctcgctat
    12181 ccgagacgaa gaccctgatc caactccgca cacggcagtt tgccaaacgt caaatgacct
    12241 ggttccgaaa ccagctttcg ttgacctgga ttgacctcgg cgcggaagaa gccgccgccg
    12301 ccgtggctat gatccttaaa caactttgat cccatcccag gctgcctccg ggatttgcgg
    12361 cggactggcc ggcatcagta gcgtttgcaa gggcaataat cttctggata tcggagaatc
    12421 ggggagtgtg ccgccgtttt tgccttgcgc acgtcaacgc tgctgagccg aaacgattca
    12481 gctttcaact ccaattcgat cccacagaat gggtaagtcc ctggttttac ggacgaaagg
    12541 cattcgcgaa gtaaaagcca ttcgggcagg ctcccgtctt tgaaaaacca ccttttgtcc
    12601 gctccggact cataactagc tgtttttaca ggacttaata attacctcac cgtttcctct
    12661 cttcgtttcg ccctttccag gcacccagct tcaccattca gatatcgtga aaatacgcta
    12721 cttccaggcc gtgtacaagg ggtgtacaag ggatgctcac cgggagttca tagggtttta
    12781 ggtagtacat ccggtgagca tcccttgtac accccttgta cacggctcat aggcaggggg
    12841 aaatcaaggc gagcctatgg tcagccaaag gcggcatgat gcaggttttg cccctatttt
    12901 ggccctctct atgcctaaat cattgatttt atcgctgaaa ggttcgaggt tccgtagcca
    12961 tgctcttgaa ttggctgaat aaagagttcg ccggggctac ggcttggacg gtcaagctcg
    13021 cccaggctcc gatggaacgg gggcgggcag ttgaactacc caactgcatc gttccggctt
    13081 aagcgggcgg ggggtgcctt gtccactttt cctgataacc ggagcaaaag cagggcatcg
    13141 tctttccagc ggtcgttttc tcctcttgtt gaggtggtgt taataatttt atggtgccag
    13201 gcgccggaac cggcttcaat gcgggcagac cataatccgg gacggctttg cccgttcgac
    13261 attggtgcgc aattatgaag cgacgtgaat tcctcaaagc ctcgatcgcc gtgcccgccc
    13321 ttgcggggct gagctgcgct tcgacgcaga accagggcgg caccgctgcc cgtagcggcg
    13381 gcgcccggga gtattacgaa ctgagggagt atcacttaaa aagcgaagcc gagcgggggt
    13441 tggtggatgg ttatctcagc aaggccctta ttccgtcatt gaaccgccgg ggctgcgaac
    13501 cggtgggggt gttcgcgcaa caagaacggc ctggcgctcc cgcgcccggc gaggtcaacg
    13561 acccccggtc ggtgctggtg cttatcccgt attcggcctt gtcggcattc ccctcggttc
    13621 tcgccgggct ggatgcggat gcggagtatc tggcggccgg ggcggagtat ttgcagtcgc
    13681 cgttgacccg gccggccttc caacggattg acagttggct gctgcaggca tttgccggga
    13741 tgccgaaggt ggagttgccg ccctattgtc gcgcgcggaa gccgcgtctg tttgaactgc
    13801 gcacgtatga aagccatagc gaggtgaagg cgcggaagaa ggttgagatg ttcaaccggg
    13861 gtgagattca actcatgcgc gaggtggggt tggggccgat cttttatggc caggggctca
    13921 ttggcggcaa cctgccgagc ctgacttatc tgttgtcggc ggaaagcacg gatgcgcacg
    13981 agcagcattg gcgcgctttt cgcgcgcatc cggcctggga acggatgaag aacgacccgc
    14041 aatacgcgga cacggtatcg aagatatccc atcggtttct ggtgccggcg ccgtattcgc
    14101 agatttagga gctctgactg gcggcggagg cgagacggat atgatgagaa gaggctggag
    14161 attgggcgtg gccgggtggc tgctctgtgc gggcggcgcg gggttggcgg ccgaggccaa
    14221 tgcgcggttt gccgtggacg tgtttggcac cgcccgggga ttgccctcca gcgcggtgct
    14281 ggcggtggcg cagacgcggg acgggtattt gtgggctggc acactggcgg gcctggcccg
    14341 gttcgacgga ttcgagttta cggtctttga cgaaaacaac acgcccggat tgagcagcag
    14401 ccaggtccga cgcctgtatg aggatcggcg agggcaactg tggattggca ccgagaacgg
    14461 agaagttgcg gtggcgcgag acggacaact gacgcgggtg caactcgggc aaaccgaggc
    14521 tccaggccgg ttaatggcga tttgcgagga caccagcgga tcggtgctgc tctacacgga
    14581 cagcggcctg ctggcccggt atctggacgg gaaaacagag gtttggcatg cggggtccgg
    14641 cgcgccaagc acctgccggg cgttgatcgc ggaggaatcg ggccgattgt ggctggggac
    14701 cgataccacc ctggtggctc tgaagccaat cccggtggtg acggatgaag tccgacccat
    14761 tcggcttaac tatctgctgg ccagccagcg gggggggtat tggcgcctgg ctaatggccg
    14821 cgtccaacga tgccaggaga accgcgtgga gcaggattgg ggcgcttatc cctgggcgac
    14881 gactctgccg attgtggcgg cgtgcgaaga tcgcgagggg aacctggtgg ttggcaccta
    14941 tggcgatggg gtgtattggt ttgacgccga aggcaaggcc acgcgcattt ccagcgaaga
    15001 cgggctttca cacaattccg ttttatcatt gacggtggat cgcgaaggct cgctctgggt
    15061 ggggacgaat ggcgggggat tgaatcgcgt gcggcgcaag cgtttcgagg tgctggaagt
    15121 ctcgcggggg gccgtggtgc aatcggtctg tgaagacgat cagggcgggg tgtgggttgg
    15181 ctgcaacggg aaccgcattt atcactgccg cgcaaatgcc acgcagcaat tccgcctcat
    15241 tccggattcg tcgcgcctgg acgtaaaccc ggatacgtta ttggatgtca aagcggtgtt
    15301 catggggctg aaccggggcg cgctgggtgg gaactggacg ttggcgggca cctggggcgc
    15361 gttgcggccg cacctgtttc aactggaata cgggcggttc gcgccgctgc ggctgccgga
    15421 atccctggat gggcgggtct cggccatttt tcaggatcgc gcggggcatc tgtggctggg
    15481 cacccagggc ggcttgctgc ggttcgacga tttaaagctc tatacgactc gcgaggggct
    15541 ttctgctgat gatgtgcggg cgattgcaga ggacccggaa ggcgacctgt ggatcgggac
    15601 cgagggcggc ggtctcaacc ggttgcatga tggccggttc acatggttca caaagacaaa
    15661 cggcctgccc ggaaacagta tttcggcgct ggaggtggat gccgagggag tgttatgggt
    15721 ggccacctcc ggcggcctgg cgcggttttt cgaggggaaa tggacgcggt attcaaaaaa
    15781 ggaagggctg gccagcaaca acctggggta tttgctggag gatgggcagg gcagcttgtg
    15841 gattggttcc aatgcggggc tcctgcggct aaaaaaggac gaactgaacg ccttcgctgg
    15901 cggcaaaagg atgactattt cgtgccgggt ttacggcgaa gcggatggcc tcccggccac
    15961 ggaatgcacg tcaggctccc agccgggcgc ggtgcgcggg cgcgatggca cgttgtggtt
    16021 cccgactatt caggggctga cctcgctgaa tccggcgcgc cttaatctga acacgaaccc
    16081 gccgccggta attattgaag cggtgctgat tgacaatcgg ttgcagaatg ccgatacgct
    16141 gcgcgcggca gccccggccg ccgtgacggt tcccccgggc agggaaacgc ttaccattcg
    16201 ttatgccagc ctcaacctgg cggcgccgga gaaagggcgg ttcaaatacc aactggaggg
    16261 atacgaaacc ggctggacgg aaaggccggg caatgtccgg gaggcgctct atcccaagct
    16321 gccgccgggc cagtatcttt tccaggttaa ggccggcaat gaagatgatg tctggaacga
    16381 aacgggcgcc acgctggcga taactgtttt gccgccgttc tggcagaagt ggtggttcat
    16441 cagcctgacc ttgattggcc tgctggggtt ggttgtgggg gcggttcact acgcctccac
    16501 tcagcggttg cagcgccagt tggcggtgct gcgccaaaag gaagccctgg agaaggagcg
    16561 ggctcgcatc gcgcgcgaca ttcacgacca gctcggcgcg agcctcacgc agatttccct
    16621 gctgggcgag ttggtggaga ttgacaagga ccagccgcgg gaggtcgaag ggcacgcccg
    16681 gcagatcgag caaacggcct tggaaaccac gcgcgccctc gatgaaattg tctggacggt
    16741 caacccctcg aacgacacgc tggacgggtt gattacgtac gtctgcaaat acgcccagga
    16801 ctacctgtca gtggcggggg tgaagtatcg cctggaagcc ccgccgcaat tacctccaat
    16861 ccccattact cccgaagtcc ggcacaacct gtttctggcg gccaaggaag ccatcactaa
    16921 cgtcgtgcgg cacgctcaag ccacggcggc cgaaatccgc atacgcctcc aggagggccg
    16981 cttcagcctc gagattcggg ataacgggcg cggcctggcg cacctggacg cccaggcggc
    17041 gcaaacgcgc aatggcctgc gtaacatgcg caaacgtatg gaagatattg gcggcagctt
    17101 ctcgattgcg tccgcgccgg aaggcggcgc cgtggtgcgg ttgagtgtcc cgttggaaag
    17161 caatgcccca taagcccgca atgaatcaaa acttatgccc attaccgtag cgatcgtaga
    17221 ggataacgac caattgcgcg gcacgctggc gcgcgtgatt aaccgggccg agggattcaa
    17281 atgtgtgagc cagtatgcca atgccgaggc ggcgctggcg ggtctgccga aagacaatcc
    17341 ggaggtggtg ctcatggaca tcaacctgcc gggcctcaac ggggttgaat gcgttcggca
    17401 gttgaagcaa ttggccccgc agattcaggc cgtcatgctt acggtgtatg aggatacgga
    17461 taacatcttc aacgcgctgg cagcgggggc gacgggctac ctgctcaagc gcaccaagag
    17521 cgcggagctg ctggagtcca ttcgcgaggt gcatagagga ggctcgccaa tgacgacgca
    17581 tatagcccgc aaggtgacgc agtcatttca gaaggctggg ccttcccagc agcccaccga
    17641 aaatctatcc cagcgcgagc aggaggtgct cgattgcctg agcaaggggt ttctctataa
    17701 agaaatcgca gacaaactgg gcatcagcta cgaaaccgtg catacctata tccgccggat
    17761 ctacgagaaa cttcaggtcc gaacccggac tgaggcggtg gccaagttcc tgcaacgtta
    17821 gtttccggcc ccggggggcg tgggttcggg ctcggtccac ttgccgtgct cgcggatgag
    17881 gtccacgagg cggtccacgg cttcggcttc ggggatgctg aacttgagcg gggtcttgcc
    17941 gacgtagagg ttgatcttgc cgggggcgcc gccgacgtag ccgaagtccg cgtcggccat
    18001 ttccccggga ccattcacga tgcagcccat gatggcgatt ttcacgccct tgaggtgctc
    18061 ggtgcgggct ttgatacggg cagtcacggt ttggaggttg aagagggtgc gcccgcagga
    18121 ggggcaggca acgtagtcgg tcttgaaaga gcggcagccg gcggcttgca ggatgttgta
    18181 ggccaggcgc agggattggc cggcgccggg ctcgccgcgc acaaggatgg catcgccaat
    18241 gccgtcggcc agcagcgagc cgatgacgac cgaggcgcgc agcagggcga ccttcggctc
    18301 cagcggcggc gagggcaggc taaggcagtc cttcaagagg atggggttgt tgcggccgag
    18361 gcgtttgagc tgtgcggcca ggaggcggaa ggcggcgatg gcgggcagcg gcacgtcgtc
    18421 cttgacggtc acgagctgcg tgtcgcagtt gactacgaat ttgcgtgggt cggcttcgag
    18481 gatgttcagg tcctcgtaaa ctgcctcggg ccttacgtcc gcgcgcgggg tgagcttggg
    18541 gacgacttta tcccaggtgg cgcgggtgac caccacccga acggtttgct gggcgccgca
    18601 tttgacgccg tcaggcagct caatctcggg cgtggggcgg cgggtgtagg agaaggggcc
    18661 gaagggaaag gctgaaggct gaaggctaga ggctaaagcc ggaaggtcgg cggccgaagt
    18721 gcggagttgg tggtccgtgg tccgtggtcc gtggtccgcg gtctgctcag ccgccaccgt
    18781 caggatagga atctgggcca ggagatcgcg gcagacttcg atttcgcgcg gggagtcctc
    18841 agtgagggag acgcggatgg tgtcgccgag gccatcgcac agaagggagc cgatgccgat
    18901 ggcgctcttg atgcggccat cctcgccttc gccagcctca gtcacgccaa ggtgcagggg
    18961 gtagttccag tccggccctt cctgttccag acgggcggcc agcaaccggt agcactggat
    19021 catgatcttg gggttgctgg ccttcatgga gaacttgaa
//
LOCUS       DAOPQG010000072        18830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3560_length_18830_cov_14.722663, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000072 DAOPQG010000000
VERSION     DAOPQG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 18830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18830
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3560_length_18830_cov_14.722663"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..2239
                     /locus_tag="PKI07_09260"
     CDS             188..2239
                     /locus_tag="PKI07_09260"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOC51279.1"
                     /translation="MHNTLTNRAADNIRILAAAMVEKAKSGHPGGAMGGADFVNILYT
                     EFLRYDPTDPGWHMRDRFFLDPGHMSPMLYAVLALSGFYTLEELKNFRQWGSPTPGHP
                     ELEVSRGVENTSGPLGQGHVSAVGAAIAERFLCARFGEWMAHKTFAFISDGGVQEEIS
                     QGAGRIAGFLGLSNLIMFFDSNDIQLSTDTRAVTLEDTARKYRAWGWSVSTIDGHDPG
                     QIRAALTQAIRQKKKPSLIIGKTIMGKGALTTDGASFERQCATHGMPLSDAGASFEKT
                     VAHLGGNPADPFAIFPEVAGLYQKAKEQKIAAAAARKAKQRRWAKAHPELAAKLELFL
                     SGQLPPLDFEEIAHKPDLATRAASGAVLGWLARKLENMIVASADLSNSDKTDGFLKQT
                     KPFVRGDFSGAFLQAGVSELTMAGLCSGMALHGGVVPACGTFFVFSDYMKPVVRLSAL
                     MGLPVKYIWTHDAFRVGEDGPTHQPVEQEAQIRLMEQLKNHKGQMSLLALRPADAAET
                     SVAWKMALANTATPTALILSRQNIKDVPARAGATRYRDALQAERGAYVALDCAGTPDV
                     VLVASGSEVSTLIGAAELLTAGKGLKARVVSVISEGLFRQQPPEYQARVLPPQLPTFG
                     LTAGLPVTLAGLVGARGKVYGLETFGYSAPAKVLDEKLGFTPAHVCERVVKYLAEYPR
                     S"
     gene            complement(2416..4611)
                     /locus_tag="PKI07_09265"
     CDS             complement(2416..4611)
                     /locus_tag="PKI07_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOC51280.1"
                     /translation="MKRSKSEPKIELKAVILAGGAKAAQGNRPMLLQKLGDQTILEYV
                     KANALQVVAAEDLYVVVGDQQKEMRALLGNRCHYVVQPQPQGTGHAVQQLAPVLQGYQ
                     GNLLILYGDTPLFRPASIRGLLNRHALRQAHLTLLTAISPRPLPYGRIIRDAAGAIVN
                     IIEETEASPAVRDIHELNVGAYLVAAPAIFAALEKLAPAPDGRYPLTDCVHELIRSGL
                     RVESYQIYDPDEIQGINTPQDLEQAEFILQKRLFRPRREEEQNQVSFGTGGWRAIIGE
                     GFTLHNVRRLCQALANEITRRGEEKRGVLIGYDRRFLSRQAGDAAAEVFAGNNIPVTL
                     LSEDAPTPLVTYATAIRNAAYGLMFTASHNPPEWNGLKVFHGDGSLLLDDETGQIQSE
                     TNRLALEEVIKIDLDIALESGIVRPGDFTNQYVDAVEALIDLDAIRQAGLKVIVDPMY
                     GVGQLTLGTILTEARCRVTFIHERHNPLFGGRSPAPNLDALRLLATHVVEDGYDLGLA
                     TDGDADRIAIVDERGEYITINDILLLLYWYMHEIRQEKGGVVRNLATTHLLDRLARRL
                     GEQCVEVPVGFKHIASAMVAHNSLLGGESSGGLTIRGHILGKDGIFASALVVEMLART
                     RQRISQLRQRVYDLTGRLYSLEDSIPSTSEMRVAVPRRLAKTPLAEIAGCPVVNVSHR
                     DGTKFYLDNDNWALVRFSGTEPILRLFAEADSPEKANALLQHLRGFVTG"
     gene            4770..5975
                     /locus_tag="PKI07_09270"
     CDS             4770..5975
                     /locus_tag="PKI07_09270"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOC51281.1"
                     /translation="MSIASHSAAGAAPPARPDPAALKAIHQLLRESPLIDGHNDLPWH
                     YRKRGNDLNALDLAGDTRKLTPPLDTDIPRLRAGGMGGQFWAVYVPPEPSGPPAVQTM
                     FEQIDLVHRMVAHYPETFGLALTAADLERIHRQGRIAALIGVEGGHAINNSLAVLRMA
                     YALGARYLTLTHVKNTDWADAAGDQPQHHGLTSFGKEVVREMNRLGMLVDLSHVTDET
                     MRDALEVSQAPVIFSHSSVRALCHSPRNVPDDVLKLTARNGGVVMVCFLPGYLTERGR
                     AALEAAEAEKARLRKLYGEGSARYQKARAEWRRRHPAAHEASLADVADHIDHVRQVAG
                     IDHVGIGSDFEGFDGAPEGLEDVSCYPALLAELLRRGYDREDLKKVAGANLLRVFREA
                     EKVSARLKR"
     gene            6043..6903
                     /locus_tag="PKI07_09275"
     CDS             6043..6903
                     /locus_tag="PKI07_09275"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HOC51282.1"
                     /translation="MEPNTAYRIVKLANGAHSVHSLAHHETFHPVVGPVAEAEALYVR
                     QLRLPERAHRQEGEFVLWDVGLGAAANALTALRATRGAPCAIRLLSFDHTLEPLAFAL
                     QHVEALGYLRGYEAPLERLLRDHHCAFQDGPQTVTWDMHLADFPSLLARPATEKLAKP
                     HGIMFDAFSPAANAAMWTLPLFSRLFRLLDPDRPCALPTYSRSTMLRVTLLLAGFHVG
                     IGHATGEKEETTLAANTPDLIAEPLDRRWLQRAHRSCSAEPLWEPVYRQARLSSATWE
                     KLQQHPQFRR"
     assembly_gap    6904..7003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7122..8459
                     /locus_tag="PKI07_09280"
     CDS             7122..8459
                     /locus_tag="PKI07_09280"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOC51283.1"
                     /translation="MKRIVASIGLVAVGASSIQAGVLPGLTTESGKPWSVSATLRGFY
                     DDNPSCLPNKMSTPGYTRSNTGFEVSPAVMFSFPWDQTSLSFGYVYSLKYYEHKLWNE
                     SKNYDQTHDFNLALTHAFSERYQLSVKDSFVIGQEPDILRYGNTFPTFRRVSGDNIRN
                     YGEVNFLAQLTPEFGVEVGYANTYYDYADDDPLYDGTTFLWPSYSGLLDQLDHRVHVD
                     GRYQILPQTIGILGYQFRQTEYIGDEVIGYDISRGDAAIKSKMRNARSHYVYAGLLHN
                     FRPDLSGSLQAGARYTDYYNNPANQNDWSPYVTASLQYTYLPESHVRLGATYDYSSTY
                     TWSPVEAGDITLNGQTFDIFITLQHRITPKLFGNVSAEFANVTYYGGTFDNRNSQYYL
                     VGLNLQYRFTPNFSAEVGYNYDLSESEYSGGMFGRSKSNYDRNRVYLGVTASY"
     gene            8524..10698
                     /locus_tag="PKI07_09285"
     CDS             8524..10698
                     /locus_tag="PKI07_09285"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOC51284.1"
                     /translation="MDPLKVAPPQEAKLHFLDYWRIIRIRKTVILAVFLLVVITATLV
                     TFILPESYSSKARIKIDRDQTDISGLADRSTLAGYDPFFIQTEFELIQSELILGKVIA
                     DLDLNTEWGRKYASGERLRTPETIALLKQRIDLRTVRGTSLIEIVVYSEKPEEAARIA
                     NAIADAYKTHRQEQRAQLSRGGIKALEERFAEQEAKVKAAQERVDNLRKELNISDAMA
                     SGDGPSPLMTAETLRKIESLRIESQAEYVRQATRLDRLKNLGKELGPEGLAQAITTDA
                     PDTLLTSLLDQLTMAEQRLIALEADYGPENTEVLKCKGLAEDLHKKIKSRVEGIMLGL
                     DTRVISLSNSLVNLQQEVGRATTNDVTQANLTRPYFEAKRALEELQRFRQVLDAKIAF
                     EKIDVELPKTLMVEVVDRAMPGLRPVRPNKPLNIALGIIIGLVVGVGLAFFIEYLDTS
                     VKTIDDVERALQCPVLGVIPQNVGLLVDEGTESPHAEAYRVLRTNLLFSRKDDKLNTI
                     AIVSAGAGEGKSTTVFNLAAVFAQSGQRTIIVDSDLRRPTLHKMLRVTNAIGLTNYLL
                     RQNTLEEVIQTTSLPTLDFLSSGKLPSSSLGILSSTQMKDLIGELKQRYDFVFFDSPP
                     IMGVSDASILASEVDMTIQVIQYRRYPQPMNIRAKQLIEKVGGNLVGIVLNNINMSQD
                     ESYYYYSGYYHDYYYRSEEQEPVVKEGAESGGKDKLDIKQKY"
     gene            10713..11345
                     /locus_tag="PKI07_09290"
     CDS             10713..11345
                     /locus_tag="PKI07_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOC51285.1"
                     /translation="MSITNIGKQLGHWGLIFGLCLAVAGLSGCRTSSPQGELAAPTGS
                     DTPAPEPEVFRIGDSLKIVYSDLPQVTPEFAGKIKEDGTITLLLNKTFTAAGKTAGEL
                     EREIRAYYVPNYYKNLTVTVTPVESTRWYYVYGEVRAPNRQIYNSRITVLQAIASAGG
                     FTDFAKKKKIKLTRVDGRTLIVNCPKAQENPSLDLEVYPGDKIYVPRRIW"
     gene            11345..11731
                     /locus_tag="PKI07_09295"
     CDS             11345..11731
                     /locus_tag="PKI07_09295"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC51286.1"
                     /translation="MVQLKVLSGKKAGTTWLARRFPIRIGRSKAADLQLEDDGVWDQH
                     LQLDFKPAEGIVLTAHPSAPATVNGHPQPQAVLRNGDGIRIGALQMQFWLSPTRQAGL
                     RWREGLTWTAVAILSLGQVALIYLLR"
     gene            complement(11747..13300)
                     /gene="purH"
                     /locus_tag="PKI07_09300"
     CDS             complement(11747..13300)
                     /gene="purH"
                     /locus_tag="PKI07_09300"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOC51287.1"
                     /translation="MEKIRRALLSVSDKTGLVPLAQALARAGIELISTGGTAKALREA
                     GLPVVDLSSYTGFPEMLEGRVKTLHPRVHGGLLYIRGNEAHEAAIRAQGIQPIDMVVV
                     NLYPFEETVARPQVALPEAIENIDIGGPSMLRSAAKNHESVTVVVDAGDYSWVARQIE
                     TAGQTTLEQRRQLAAKVFARTAAYDAAIAAHLETAFGLTATTGVPGALRIQAPLAQVL
                     RYGENPHQKAALYGGFHDSFQQLQGKELSYNNILDLTAAAALISEFAADPPTLAILKH
                     TNPCGVGQGSSLREAWDKAFATDRQAPFGGIIAVNRALDLACAEAIAGIFSEVIVAPD
                     FTAEALELLRQKKNLRILRNRESSFAAQACDVRSVGAGSYLWQERDLRVTTAADLKLV
                     SRRPPTEAERQAMLFGWRIVKHVKSNAIIFVAADRTLGIGAGQMSRVGASRIAVWKAR
                     EAGLSLTGSVVCSDAFFPFPDGLIAAAEAGATAAIQPGGSVRDAEVIAAADERDVAMA
                     FTGVRHFRH"
     gene            complement(13572..14918)
                     /locus_tag="PKI07_09305"
     CDS             complement(13572..14918)
                     /locus_tag="PKI07_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51288.1"
                     /translation="MKPITRREFTRTLAAVSTTAALSSFRVFGANDRVRLGFIGVGNR
                     GDQVLDAFLKQSDAEIVAVCDIYEPYLDFAARKVGTGPKRFKDYRQLLELKDVDAVVI
                     STPDHWHALQTIHACQAGKDVYVEKPISLRVAEGRAMVQAVKRNKRVCQSGLHRRSAA
                     MFREMAEYVRGGGLGQVTGVRTFHIQNEWPKGIGNPPDSPPPPGMDWEAWLGPARKVP
                     YNRNRGLYQFRWFFDYSGGQITNNGIHYLDLIHWALGQEAPLAVTGMGGRFAVEDNRD
                     VPDTMEALWHYPGNTLVIYTQFNANGAGAAAKPCEIEFRGTKGTLYMRSNGWEVVPEV
                     ITPNEFPRRSPTDRSSERGWRTGEAPVIEARKESGRADTADHARNFLDCIKSRAACNS
                     DLESAHRSTSTTLIANIAMQTRSYLEWDAKAEKFTNNPAANKLLRYDYRPPLKSPA"
     gene            complement(14969..15487)
                     /locus_tag="PKI07_09310"
     CDS             complement(14969..15487)
                     /locus_tag="PKI07_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51289.1"
                     /translation="MGFCEGQIMPGREPGISRPVRVSPVSRACWVAACGLVALGGLLA
                     SCGSKTELPLEAPPAQTNAAEAVAVSPELAKLTGKWERSDDGYRLEIKGIDASGKVEA
                     AYYNPNPIRVSRAAAWREQGISRIVVELNDTGYPGCLYTLEFNAQTDQLVGQYFQAAQ
                     QATYEIVFFRIE"
     gene            complement(15525..16763)
                     /locus_tag="PKI07_09315"
     CDS             complement(15525..16763)
                     /locus_tag="PKI07_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOC51290.1"
                     /translation="MTRFVIGRLGIMVAVSCLWLGANFITRAAEGGAAARTQIRVDAN
                     FPGGNIVVDSIAGDLVTLRPDLRDTEGDWFYWYFRVRGGAGRTLQFSFSRSNPIGTRG
                     PALSLDRGKSWSWLGTNAVAKQTFTCALPRKAREARFCFAIPYLEHNLKDFLKRHRRH
                     PNLKAETLCQTSGGRRVELLRLGRLDGKAGHCVVLACRHHACEMIASYSLEGLMEAVL
                     DAADVRWLREQVEFFVVPFMDKDGVEAGDQGKNRRPYDHNRDYQGESLYASVRAMREL
                     ITGRLGARNIALALDMHCPHIRGAHNEHIYFVGGPAEDNWARVGELARVLEAVQRGPL
                     HYRAADNLPYGQAWNTLAGPARTFGRWMADQPNVRIGTTIEIPYANVGETTVTAENAR
                     AFGHDLARALARYLPSRIAD"
     gene            17151..17366
                     /locus_tag="PKI07_09320"
     CDS             17151..17366
                     /locus_tag="PKI07_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51291.1"
                     /translation="MNHQLHIGFETRPDRRTARCIRKRSHRAQWWFERMREIVAEAQD
                     WPPVAPPSAADEASVAPMASGEPSDHT"
     gene            complement(17514..18680)
                     /locus_tag="PKI07_09325"
     CDS             complement(17514..18680)
                     /locus_tag="PKI07_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51292.1"
                     /translation="MKNSTSLTVLAVVTLLLAVAAAQAQQHRAVRLGHPLTRFAPPLE
                     RPEQLRALFADEKLKADVAEILRQAGWEGEVEDLRQAAAVAPITELSLPVGTRMPYMS
                     SRKNGEPEALIDVLWDGTEPISAYAFDFTSKGRRYRCITPRPCSNFYTVDLGPALPEL
                     ALGCSAPGQALVGRRFEVCLTVTNLGTALEPQPTLRLPVPPEAVLAGVTDQGDPADGN
                     IVWKLPALQPGTAKQVCATFVAHQAATAAFAATVEGASGPAVSTRCETLVAGIPAILL
                     ETADLEDPIEVGKEVVYEIKVTNQGSAPGTNLKVTCALPESEEFVSGTGPTAVQGQGG
                     TVTMETVPVLAPKAQAVWQVTVKALKPDDARFKVFIRSDQFEKPIQKDEATQLY"
BASE COUNT         3591 a         5829 c         5690 g         3620 t
ORIGIN      
        1 tgaagcccta gacgctttcg ccccggcctc ctgagcccga aaagcggttt tggggttgga
       61 agaacctttt ccccctcagc caccgaaacg caggcgccga taacctaggg ccctggccgc
      121 aacgatgccg cccggtcgaa agcgcttgat aattgttgct ggaagcaaat aattcccgcc
      181 gaacaacatg cataatacgc ttactaatcg ggccgctgac aatatccgca ttcttgccgc
      241 cgccatggtg gaaaaagcca aatccggtca tccgggcggg gcgatgggcg gcgccgactt
      301 cgtcaatatt ctctacaccg agttcctgcg ttatgatccc acggatccgg gctggcatat
      361 gcgagaccgg tttttcctgg atccgggcca catgtcgccg atgctctacg ccgtgctggc
      421 gctcagcgga ttctacacgc tggaagagtt gaagaacttc cgccaatggg gcagccccac
      481 gcccgggcat ccggagctgg aggtgagccg cggcgtggag aacacctccg ggccgctggg
      541 ccaggggcat gtcagcgcgg tcggcgccgc cattgcggaa cgctttttgt gcgcgcggtt
      601 tggcgaatgg atggcccaca aaacctttgc ctttatttcg gatggcggtg tgcaggagga
      661 gatttcgcag ggggcgggcc ggattgccgg ttttctgggc ctgagcaacc tgatcatgtt
      721 cttcgactcc aatgatattc agctttccac cgatacccgc gcggtgacgc tcgaagatac
      781 cgcccggaag taccgggcct ggggttggag cgtcagcacc attgacggcc atgacccggg
      841 ccagattcgc gccgccttaa cccaagcgat ccggcaaaag aagaagccca gcctgatcat
      901 cggcaagacc atcatgggca agggcgcgct gaccaccgac ggcgccagct tcgaacgcca
      961 gtgcgccacg catgggatgc cgttaagtga tgcgggcgct tctttcgaaa agaccgtggc
     1021 ccacctgggg ggcaacccgg ccgatccgtt cgccattttc cccgaggtgg ccgggctgta
     1081 ccagaaggcc aaagaacaaa agattgccgc ggccgccgcc cgcaaggcca aacagcggcg
     1141 ctgggccaag gcccatcccg aactggccgc caagctggag ttgtttctct ccggccagct
     1201 gccgccgctc gactttgagg aaatcgcgca caagcccgac ctcgccaccc gcgccgcctc
     1261 gggcgcggtg ctgggctggc tggcgcggaa attggagaac atgattgtcg cttccgccga
     1321 cctgtccaac agcgacaaga ccgacggctt tctcaaacaa acgaagccat ttgtccgggg
     1381 cgatttcagc ggggcgtttc tgcaggccgg agtgagcgag ctgacgatgg cggggctctg
     1441 cagcggcatg gcgctgcacg gcggcgtcgt gcccgcgtgc ggcacctttt ttgtttttag
     1501 cgattacatg aagccggtgg ttcgcctgtc ggcgctgatg gggttgccgg tcaagtatat
     1561 ctggacgcac gacgccttcc gggtgggcga ggacggcccc acgcatcagc cggtcgagca
     1621 ggaggcccaa atccgcctca tggagcaact caagaaccac aagggccaga tgagcctgct
     1681 ggcgctgcgg cccgcggatg ccgccgagac gagcgtggcc tggaagatgg cgctggcgaa
     1741 caccgccacg cccacagcgc tgattctctc gcggcagaat atcaaggatg ttccggcgcg
     1801 ggccggcgcc acccgctacc gggatgcctt gcaggcggag cggggcgcgt atgttgcgct
     1861 tgactgcgcc ggcacgccgg atgtggtgct ggtggccagc gggtcggagg tgtccacgct
     1921 cattggcgcc gccgagctgc ttaccgccgg gaaagggctc aaggcgcgcg tggtatccgt
     1981 catttcggag gggcttttcc gccagcagcc tcccgaatac caggctcgcg tgctgccgcc
     2041 gcaactgccc accttcggtc tgaccgccgg gttgcccgtc acgctggcgg gcctggtggg
     2101 tgcccggggc aaagtttacg gtttggaaac cttcggctac tcagccccgg ccaaggtgct
     2161 ggacgaaaag ctcggcttca ccccggccca tgtttgcgag cgggtggtaa agtacctggc
     2221 ggaatacccg cggagttgaa cggttccagc gcggtccggg cggctggcgg cgcacggccc
     2281 ggtttttacg ccagcccgga gcggagttgg gcggccggga aatatcgggc gggaggggag
     2341 ccattccacg gcggaggctt ccgcggctgg cagagcaggc cccggagccg gggttcgcag
     2401 cgtgtgctga atgaattagc cggtcacaaa accgcgcaag tgctggagga gcgcgttggc
     2461 cttttccgga gaatcggctt ccgcgaacaa gcgcaggatc ggctcggtgc cggagaaacg
     2521 caccagcgcc cagttgtcgt tgtccaggta gaacttggtt ccgtcccggt gggagacgtt
     2581 cacgaccggg cagccggcga tctccgccag cggcgttttg gccaggcgcc ggggcaccgc
     2641 cacccgcatt tcggaggtcg aggggatgct gtcttcgagg ctgtaaaggc gccccgtgag
     2701 atcatagacc cgctgccgca gttgggaaat ccgctggcgc gtgcgcgcga gcatttcgac
     2761 gacgagggcc gaggcgaaaa tgccgtcctt gcccaggatg tgcccgcgaa tggtcaaccc
     2821 gccgctggat tcgcctccca ggagggaatt atgcgcgacc attgcgctgg cgatatgctt
     2881 gaacccgacg gggacttcga cgcattgctc gccgagccgc cgcgcgagcc ggtcgagcaa
     2941 gtgggtggtg gccaggttgc gcaccacgcc gcctttctcc tggcggattt cgtgcatgta
     3001 ccaatacaac aggagcagga tgtcattgat ggtgatgtat tcgccgcgct cgtccacgat
     3061 ggcgatgcgg tcggcgtcgc catcggtggc cagtcccagg tcgtagccat cttcgacgac
     3121 gtgcgtcgcc agcagccgca gggcgtccag gttcggcgcc ggcgagcgcc cgccaaacag
     3181 cgggttatgc cgttcgtgga tgaatgtcac ccggcagcgc gcttcggtga ggatggtgcc
     3241 cagcgtgagc tggcccacgc cgtacatcgg gtccacgatc accttgaggc cggcctggcg
     3301 gatggcgtcg aggtctatca gggcctccac cgcgtccacg tactggttgg tgaaatcgcc
     3361 gggccggaca atgccgcttt ccagcgcgat gtccaaatca atcttgatga cctcctcgag
     3421 ggcgagccgg ttggtctcgg attgtatctg gccggtttcg tcgtccagga gcagcgagcc
     3481 gtcgccgtgg aagactttga ggccattcca ctcgggcggg ttgtggctgg cggtgaacat
     3541 gaggccgtag gccgcattgc ggatcgccgt ggcgtaggtg acgagcggcg tgggggcgtc
     3601 ctcgctcagc aaggtcaccg gaatgttgtt tccggcaaag acctcggcgg cggcatcccc
     3661 ggcctggcgg gacaggaacc ggcggtcata gccgatcagc acgccgcgct tttcctcgcc
     3721 gcgccgcgta atctcattgg ccagcgcctg gcagaggcgg cggacgttgt ggagcgtgaa
     3781 gccctcgccg atgatcgccc gccagccgcc ggtgccaaag gagacctggt tttgctcctc
     3841 ctcgcggcgg ggccggaaga ggcgtttttg caggatgaac tcggcttgct ccaggtcctg
     3901 cggcgtgttg atgccctgga tttcgtccgg gtcgtaaatc tgatagctct ccacccgcaa
     3961 acccgagcga atgagttcgt ggacacagtc cgtcaacgga taccggccat ccggcgccgg
     4021 ggcgagtttt tccagcgcgg caaagatggc tggcgccgct accaggtagg cgcccacgtt
     4081 gagctcgtga atatcccgca cggcgggcga ggcttcggtc tcctcgatga tgtttacaat
     4141 ggcgccggcg gcatcgcgaa tgatgcgccc gtaaggcagc ggccgggggg agatggcggt
     4201 caacagggtc agatgcgcct ggcgcaaggc atggcggttg agcaaccccc gaatggacgc
     4261 cgggcggaac agcggggtat cgccgtacag gattaacagg tttccttgat agccttggag
     4321 cacgggggcc agttgttgca ccgcatgccc ggtgccctgc ggctggggct gcacgacata
     4381 gtggcaccgg ttgcccagca aggcgcgcat ttccttttgc tgatcgccga ccacgacata
     4441 gagatcctcc gccgcaacga cctggagcgc gttggctttg acgtattcga ggattgtctg
     4501 gtcgccgagt ttctgcagca gcattggccg gttcccctgg gcggcttttg cgccgccggc
     4561 gaggatgacg gcctttaatt caatcttcgg ctctgatttt gatcgcttca tggtgccggc
     4621 gggcatccta cgcggttgcc tccggaatgc aaactccgtt gttggggcgg ggaaacgcgc
     4681 ggcggcttga ccccggccct gcttcgcttc actctggcgc tattatgagt ctgtgctccc
     4741 agcgcggggc tgttatcctg tccttgttta tgagtattgc cagccactcc gccgccggcg
     4801 cggctccgcc ggcccggccc gatcccgccg ccctgaaagc gatccatcag ctattgcgcg
     4861 agtcgccgct tattgacggc cacaacgacc tcccctggca ttaccgcaag cgtggcaatg
     4921 acctgaacgc cctggacctg gcgggcgaca cccgcaagct cacccccccg ctggataccg
     4981 atattccccg cctgcgggcg ggggggatgg gagggcagtt ctgggcggtg tatgtccccc
     5041 cggaaccttc cggcccgccc gccgtgcaga cgatgttcga gcagattgac ctcgtgcatc
     5101 ggatggtggc gcattatccc gagacctttg ggctggcgct gacggctgcc gacctcgaac
     5161 gcattcatcg ccagggcagg attgccgcct tgatcggcgt ggaaggcggc catgcgatca
     5221 acaactcgct ggcggtcttg cggatggcct atgccctggg cgcgcgctac ctgaccctca
     5281 cacacgtcaa gaacactgac tgggccgatg ccgccggcga ccaaccgcag catcatggcc
     5341 tgacttcctt cggcaaggag gttgtccgcg aaatgaaccg cctgggcatg ctggtggatt
     5401 tgtcgcacgt cacggatgag accatgcgcg atgccttgga agtcagtcag gccccggtga
     5461 ttttctccca ttcctcggtg cgggcgctat gccattcccc acgcaacgtc ccggacgatg
     5521 tcctgaaact gacggcgcgc aatggcgggg tggtgatggt gtgttttctt cccggctatc
     5581 tcaccgaacg ggggcgcgcg gcgctggagg cggcggaggc cgagaaggcg cgcctccgca
     5641 agctctacgg cgaaggttcg gcgcgctacc agaaggcgcg ggcggagtgg cggcggcggc
     5701 atcccgcggc gcacgaagcc agcctggccg acgtggcgga tcatattgac cacgtgcgcc
     5761 aggtggcggg gatagaccac gtgggcatcg ggtcggattt cgagggcttc gacggcgcgc
     5821 ccgaggggct ggaagatgtc tcctgctacc cggcattgct ggcggaattg ctgcgccgcg
     5881 gctacgaccg cgaggacctc aaaaaagtgg ccggcgccaa cttgctgcgc gtctttcggg
     5941 aggcggagaa agtgtccgcg cggctgaaac gctgaacgcg gcgcgtgggg cggcttgcgt
     6001 tggaacacct ccccgcgcct tgcgtcccgc aggcgagttc ccatggaacc caataccgca
     6061 taccgaatcg tcaagctcgc caacggcgcg catagcgtcc attcgctggc ccatcacgag
     6121 acgtttcatc cggtcgtcgg cccggtcgcc gaagccgagg ctttgtatgt ccggcaactg
     6181 cgtctgcccg agcgggcgca ccgccaggag ggggagtttg tgctgtggga tgtcggcctg
     6241 ggcgccgccg ccaacgcgct gacggctctg cgcgcgaccc gcggcgcgcc ctgcgctatt
     6301 cgcctgttga gctttgacca taccctcgaa ccgctggcct ttgcgctgca gcacgtcgaa
     6361 gcactcggct atctgcgggg atatgaagcg cccctggagc gccttctgcg cgaccaccac
     6421 tgcgccttcc aggacggccc gcaaaccgta acgtgggata tgcacctggc cgacttccca
     6481 tccctgctcg cccggccggc cacggagaag cttgccaagc cgcatgggat tatgttcgac
     6541 gcgttctctc cggcggccaa tgcggcgatg tggacgctgc cgctcttctc gcgccttttc
     6601 cgcctgctgg acccggaccg gccctgcgcg ctgccgactt actcgcgcag cacgatgctg
     6661 cgcgtcaccc tgctgttggc ggggtttcat gttggcatcg gccacgccac cggcgaaaag
     6721 gaggaaacca ccctcgcggc gaataccccc gacctcatcg ccgaaccctt ggatcgccgc
     6781 tggctgcagc gggcgcaccg ttcctgcagt gccgaaccct tgtgggagcc ggtctatcgt
     6841 caggctcggc tttcctccgc cacctgggaa aagctgcagc agcaccccca attccgccgt
     6901 tagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgggggc ttaaaaaaag
     7021 tgtaaagatt tatttgacag cccccgggag cgtgcggtaa aaagggtgag ttagcagttg
     7081 gcattgtgta actttgggtg tacaaacact gaataaattc tatgaaacga atcgttgcct
     7141 ccataggttt ggtagccgtc ggcgcctcta gtattcaagc gggagtcctg ccgggcttaa
     7201 cgacagaatc cggcaagccc tggagtgtct ccgcgacgct ccgtgggttt tatgatgata
     7261 accccagttg cctccctaac aaaatgtcaa cgccggggta tacgcgcagc aataccggat
     7321 ttgaagtcag ccccgccgtg atgttcagtt ttccgtggga tcaaaccagc ctgagtttcg
     7381 gctatgtgta ttccctcaag tactatgaac acaagctgtg gaacgagtct aagaactacg
     7441 atcagaccca cgactttaac ttggcgctca cgcatgcatt cagcgagcga tatcagctca
     7501 gcgtaaagga ttccttcgtc attggccagg agcctgatat cctgcggtat gggaatactt
     7561 tcccgacctt ccgccgcgtt tccggggata atatccgcaa ttacggcgag gtcaatttcc
     7621 tggcgcagtt gacgccggaa tttggggtgg aagtgggata cgccaatacg tattatgact
     7681 acgcggatga cgatccgctt tatgatggaa ccactttctt gtggcccagc tattctggtt
     7741 tgttggatca attggaccac cgggtgcatg ttgacggtcg ttatcagatt ttgccgcaaa
     7801 ctatcggcat tctgggctac cagttccggc agacggaata tatcggagac gaggtgattg
     7861 gttatgatat cagccgggga gatgccgcaa taaagagtaa aatgcgcaat gcccgttcgc
     7921 attatgttta tgcggggctc ttgcataatt tccgaccgga cttgagcggc tcgcttcagg
     7981 cgggagcccg ttataccgac tactacaaca accctgccaa tcaaaacgat tggagccctt
     8041 acgtgaccgc aagcctgcag tacacctacc tgccggaaag ccatgtgcgg cttggggcga
     8101 cctatgacta tagctcgacc tatacgtgga gccccgtcga ggcgggcgat attacgctga
     8161 atgggcaaac gttcgatatc ttcatcacgc tgcagcaccg catcacgccg aagcttttcg
     8221 gcaatgtgag cgcggagttt gcgaatgtca cctattacgg aggaaccttc gacaaccgga
     8281 attcgcagta ttatctggtt ggcttgaatc tgcagtatcg cttcactcct aacttctccg
     8341 cggaagtcgg gtacaactat gacctttcgg agagcgaata ttccggcgga atgtttggcc
     8401 ggagcaaatc taattatgat cggaaccggg tttacctcgg agtgactgcc agctactaaa
     8461 ccaagcttga ttcgatgacg gggggctagg ctgtcacaac tggcccccct tattttttaa
     8521 tatatggatc cattgaaagt tgctccgcca caggaagcca aattgcattt cctggactat
     8581 tggcggatca ttcgtattcg caagacggtt atcctggctg tctttttgct ggtcgtaatc
     8641 actgccacgc tggttacctt cattttgccc gaatcctatt cgagcaaggc acggatcaag
     8701 attgaccgcg atcagacgga tattagcggt ctggcggatc gctcgacgct ggccggttat
     8761 gatcccttct ttatccagac tgaatttgag ttgatccaat cggagcttat tctcggcaag
     8821 gtgattgccg atctcgatct gaacacggaa tggggccgga agtacgccag cggggaacgc
     8881 cttcggacgc cggaaacgat tgcgctctta aagcaacgga ttgacttgcg cactgtgcgc
     8941 ggcaccagcc tgattgaaat tgttgtctat agtgagaaac ccgaagaggc tgccaggatt
     9001 gccaacgcca ttgcggatgc gtacaagact caccgtcagg agcaacgcgc acaactgagc
     9061 cggggcggaa ttaaggcgct ggaagagcgc ttcgcggaac aggaggcaaa ggtcaaagcc
     9121 gcccaggagc gagtggataa cttgcgtaag gagctgaaca tctctgatgc gatggccagc
     9181 ggcgatggac cgtcgccctt gatgaccgcc gagacgctgc gcaagattga aagcctccgc
     9241 attgaaagcc aggccgaata cgtccggcaa gccacccggc tggaccgttt gaagaatctc
     9301 ggtaaggaac ttgggccgga aggacttgcc caggctatca caaccgatgc tccggacacg
     9361 ctgcttacct ccctgctcga tcaactgact atggccgagc agcgattaat cgcgttggaa
     9421 gcagattacg gcccggagaa caccgaggtg cttaaatgca agggcttggc ggaggacttg
     9481 cacaaaaaga tcaaaagccg ggtggagggc attatgctgg gcttggacac gcgggtgatt
     9541 tcattgagca acagcctagt caacttgcag caggaagtcg ggcgggccac aaccaacgac
     9601 gtcacgcagg ccaatttgac gcggccatat tttgaagcca agcgcgcact ggaggaattg
     9661 cagcgcttcc gccaggtgct cgatgcgaaa attgcctttg aaaagattga cgtggaattg
     9721 cccaaaaccc tgatggttga agtggtggac cgcgcgatgc ccggtttgcg accggtgcgt
     9781 cccaacaagc cgcttaatat cgctctgggc atcatcatcg gcctggtagt gggggtcggg
     9841 ttggcgttct ttattgaata tctggatacc agcgtaaaaa cgattgacga tgtagagcga
     9901 gcgctgcagt gcccggtgtt gggcgttatc cctcaaaacg tgggcttgct ggtggacgaa
     9961 ggaaccgaaa gcccgcacgc ggaggcttac cgtgtattgc gcaccaatct gctattctcg
    10021 cgcaaggacg ataaactgaa taccatcgcc atcgtcagcg cgggtgcggg tgaaggcaaa
    10081 tcaaccacag tctttaacct cgccgcggtc tttgcccaaa gcgggcagcg gactatcatc
    10141 gtggattccg acctgcgccg gccgacgctg cataaaatgc tgcgcgtgac caacgccatt
    10201 gggctgacca actatctctt gcggcaaaat acgcttgaag aagtcattca gacgaccagc
    10261 ttgccgaccc tggatttcct gtcgagcggc aaactgccca gcagttcgct gggcatcttg
    10321 agttccacgc agatgaagga tttgatcggg gaattaaagc agcgctacga ctttgtgttc
    10381 tttgattcgc cgccgattat gggggtgagc gatgcgtcca ttctcgccag cgaggtggat
    10441 atgaccattc aggtcattca gtatcgccgc tatccgcaac cgatgaacat ccgcgccaaa
    10501 cagttaatcg agaaggttgg cggcaatctg gtgggcatcg tgttgaacaa catcaacatg
    10561 tcgcaggatg aaagctacta ctactacagc ggctattacc acgactatta ctatcgcagt
    10621 gaggaacagg aaccggttgt gaaggaaggg gcggaatccg gaggcaaaga caaattggat
    10681 atcaagcaga agtactaaga gggatcgttt gcatgagcat cacgaatatc ggtaaacaat
    10741 tgggtcattg ggggttgatc tttggtttat gcctggcggt ggccggcctg agtggttgcc
    10801 gcacgtcatc accacaagga gaactggcag ccccaacggg gagcgatacc ccggcgccag
    10861 agccggaagt gttccgtatc ggcgattctt tgaagatcgt ttattccgat ctgccgcagg
    10921 tgactccgga gtttgccgga aagatcaagg aagacggcac catcaccttg ctcctgaaca
    10981 aaaccttcac cgcggccggt aaaactgcag gcgagttgga gcgggagatc cgggcctatt
    11041 atgtgcccaa ctactacaag aacctgaccg tcaccgtgac gcccgtggaa agcacgcgct
    11101 ggtactacgt ttatggcgag gtgagagctc ccaatcggca gatctataac agccgcatca
    11161 cggtcttgca ggcgatcgct tccgccggcg ggttcaccga tttcgccaag aagaaaaaaa
    11221 tcaagctaac ccgcgtggac ggtcgcactc ttatcgttaa ctgccccaag gcccaggaaa
    11281 acccctccct ggacctggaa gtttatcccg gcgataagat ctatgttccg cgccggattt
    11341 ggtgatggtc cagcttaagg tgttgtccgg aaagaaagcg ggcaccactt ggctggctcg
    11401 gcgatttccc atacgaatcg gccgctccaa agcggctgat ctgcaactgg aggatgacgg
    11461 tgtttgggat cagcacctgc agctggactt caaacccgcg gaagggatcg tcctgaccgc
    11521 tcatcccagc gcccctgcca cagttaatgg gcacccgcaa ccgcaggccg tgctacgcaa
    11581 cggagatggt attcgcattg gcgcgctcca gatgcagttc tggctcagcc caacccgcca
    11641 agccgggttg cgctggcggg aaggattgac ctggacggca gtcgccattc tttccctggg
    11701 ccaggttgcc ctgatttacc tgctccggta gttccgacgg cccaggttaa tggcgaaaat
    11761 gccgcacgcc ggtaaacgcc atggcaacgt cccgctcgtc ggccgccgca atcacctcgg
    11821 catcgcgcac tgaacctccc ggttgaatcg ccgccgtcgc gcccgcctcg gccgccgcaa
    11881 tcagcccgtc gggaaaagga aagaaggcat cgctgcaaac cacgctgccc gtcagcgaca
    11941 aacccgcttc ccgagccttc cacaccgcga tgcggctcgc gcccacccgg ctcatctggc
    12001 ccgcgccaat gcccagcgtt cggtcggccg caacaaagat gatggcgttg gacttcacat
    12061 gcttcactat gcgccaaccg aataacatgg cctggcgctc ggcttccgtg ggcgggcgcc
    12121 gagaaaccaa cttgagatca gcggcggtcg ttacccgcaa atcccgttcc tgccataagt
    12181 aggagcctgc gcccacactg cgcacgtcgc atgcctgcgc ggcaaacgaa ctttcccgat
    12241 tgcgcaggat gcgcagattc tttttctgcc gcagcagttc gagcgcctca gccgtgaaat
    12301 caggggcaac aattacttca ctgaaaatgc ccgctattgc ctccgcacag gccaggtcaa
    12361 gcgcacggtt cacggctatg atgccgccaa acggcgcctg ccggtcagtt gcaaatgctt
    12421 tatcccaggc ttcccgcaaa ctcgatcctt gtcccacacc acaaggatta gtgtgcttga
    12481 gaatggccaa ggtgggcggg tccgccgcaa attccgaaat cagggccgcc gctgcggtta
    12541 aatcgaggat gttattataa gagagttcct tgccctgtag ttgctgaaaa gaatcatgaa
    12601 aaccgccata cagcgccgct ttctgatgtg gattctcgcc gtagcgcaac acctgcgcca
    12661 gcggggcttg aatccgcaac gccccgggga cgccagtcgt ggccgtcagg ccaaatgccg
    12721 tttccaagtg cgctgcgatc gcggcatcgt aggccgcggt gcgcgcaaac accttggccg
    12781 ccagttggcg ccgttgttcc aatgtggtct gcccggccgt ttctatctgc cgggcaaccc
    12841 acgaatagtc tccggcatcc accaccaccg tcacactttc gtgattcttg gccgcgctgc
    12901 gcagcatgga tggtccccca atgtcaatgt tttcaatggc ctcgggcagg gcaacctgcg
    12961 gtcgggcaac agtttcctca aacggataca ggttgaccac caccatatca atcggctgaa
    13021 tgccctgcgc ccggatcgct gcctcatgtg cttcattgcc gcgaatgtaa agcaaacctc
    13081 cgtgaacccg gggatgaagc gtcttaaccc gaccttccag catttcggga aagccagtgt
    13141 agctgctcag atcgacaacc ggcaacccgg cttcgcgcaa cgctttggct gttccgccgg
    13201 tggaaatcaa ctcaattccc gccctggcca acgcctgggc cagcgggacc agcccggtct
    13261 tatcagaaac tgagagcagc gcgcgtcgaa ttttctccat aaccaggcca caccttctct
    13321 gcctgtgccg gctctgtcaa tcggtaaagc ttcctttgcg cgcgttctct cgaactgcgc
    13381 caaccctagc cgcagcgcaa agcagccagc atttccggct agttgcgggg cttagcgaaa
    13441 gccgaaaaca ggtcagcaac taactctcaa accagttgcc tattcttcct gtcgtccggc
    13501 caaccagcca cctgaacaga tcccgcacgc cggaaccgca gaccttaccg cagccgctgc
    13561 ccttgcgccg ttcaggccgg gcttttgagc ggtgggcggt agtcgtagcg caggagcttg
    13621 ttggccgcgg gattgtttgt aaacttctcc gccttcgcat cccattccag atatgaccgg
    13681 gtctgcatgg caatgttggc gataagcgtg gtgctggtgc tccggtgggc gctttccaaa
    13741 tccgaattgc aggccgcccg gctcttgatg caatcaagaa agttgcgggc atggtcggcg
    13801 gtatccgcgc gcccgctctc ttttcgggcc tcgatcaccg gcgcttcgcc cgtgcgccag
    13861 ccgcgttcgc tgctgcggtc ggtaggcgag cggcgcggaa actcgttggg cgtgataacc
    13921 tccggcacaa cttcccagcc gttgctccgc atgtagagcg tgcctttcgt gccgcgaaac
    13981 tcgatttcgc acggtttggc ggcggccccc gcgccattgg cattgaactg ggtatagata
    14041 acgagcgtat ttcccggata atgccagagc gcctccatgg tatccggcac gtcgcggttg
    14101 tcctcgaccg caaaccgccc ccccatgccc gtcacagcca gcggggcttc ctgccccagt
    14161 gcccaatgga tcaaatcgag atagtggatg ccgttattcg tgatctggcc gccgctgtag
    14221 tcgaagaacc aacggaattg ataaagcccg cgattccggt tgtagggcac ctttcgcgcc
    14281 ggccccagcc atgcttccca gtccatgccg ggcgggggcg ggctgtcggg cggattgccg
    14341 atgcccttgg gccattcgtt ttgaatgtgg aaggtgcgca ctcccgtgac ctggcccaat
    14401 ccgccaccgc ggacatactc cgccatctcg cgaaacatgg ccgccgagcg ccggtgcaaa
    14461 cccgattgac aaacccgctt attccgcttt accgcctgaa ccatcgcccg cccctcggcg
    14521 acgcgcaacg agatcggctt ctcgacatat acatctttcc cggcctggca ggcatggatg
    14581 gtttgcaacg catgccagtg atcgggcgtg gagatgacca ccgcatccac gtccttcaat
    14641 tcaagcagtt ggcggtagtc tttgaatcgt ttagggccgg tgccaacctt ccgggcagca
    14701 aaatcaagat acggctcgta gatgtcgcaa acggccacga tttccgcatc gctttgcttc
    14761 aagaaggcgt ccagcacctg gtcgccgcgg ttgcccacgc caatgaagcc cagccgcaca
    14821 cggtcgttgg cgccaaatac gcggaaggac gaaagggccg ccgtcgtgct gaccgcggca
    14881 agggtccggg taaactcccg gcgggtgatt ggtttcataa aagtcgagtc agacacagcc
    14941 tgaggttgat ggttggggcg gacgggaatc attcgatccg gaaaaataca atctcgtagg
    15001 tcgcctgctg cgcggcttgg aaatattgcc cgaccaactg gtccgtctgc gcgttgaact
    15061 ccagcgtgta taggcaaccg ggatagcccg tatcattcaa ctcaacgacg atccggctga
    15121 ttccctgctc gcgccaggcc gcggcgcgcg acacccggat cgggttaggg ttgtagtagg
    15181 cggcttccac cttgccgctg gcgtcaatgc cttttatctc cagcctgtag ccgtcgtccg
    15241 aacgctccca tttgcccgtc agcttcgcga gttccggcga taccgccacc gcctccgccg
    15301 cgttggtttg tgcgggaggc gcctccaagg gcagctcggt tttactgccg caggatgcca
    15361 gcaagccgcc aagcgccacc aagccgcaag ccgccaccca acaggcccgg gacaccgggc
    15421 tcacccggac gggccgggaa atgccaggct cccggccagg catgatttgg ccttcgcaaa
    15481 atcccataag ctcgcaacaa atatacgtgc cggggcgcgg cgcctcaatc agcaatccgt
    15541 gatggaagat accgcgccaa cgcccgggcc agatcatgcc caaaggcgcg cgcattttca
    15601 gccgtgaccg ttgtctctcc gacattcgcg tagggaatct cgatcgtggt gccaatgcgg
    15661 acgtttggtt gatcggccat ccagcgcccg aaggtgcgcg ccgggcctgc cagcgtgttc
    15721 caggcctggc catacggaag gttgtccgcc gcgcgatagt gaagcggccc gcgctgcacc
    15781 gcctccagca cccgggccaa ctcgcccacc cgcgcccagt tatcctccgc cggcccgccc
    15841 acgaagtaaa tatgctcgtt atgcgcgccg cgaatatgcg ggcagtgcat atccaatgcc
    15901 agcgcgatgt ttcgcgcgcc gagccggccg gtgatcagct cccgcatcgc ccgcacggaa
    15961 gcgtaaaggc tctcgccctg gtaatcgcgg ttgtggtcgt agggacggcg attcttgccc
    16021 tggtcgcccg cttcaactcc atccttgtcc atgaacggca ccacaaagaa ctccacctgc
    16081 tcgcgcaacc accgaacatc cgcagcgtca agcaccgctt ccatgagacc ctccaggctg
    16141 tagctggcaa tcatttcgca ggcatgatgg cggcacgcca ggacgacgca gtgtccggcc
    16201 ttcccatcca accgtcccag ccgcagcagt tccacccggc gtcccccgga ggtctggcag
    16261 agagtttccg cctttagatt ggggtggcgc cgatggcgtt tcaggaagtc cttgagattg
    16321 tgctccagat atggaatagc aaaacaaaac cgcgcctcgc gcgccttgcg gggaagggcg
    16381 caagtaaagg tctgctttgc cacggcattg gtgcccagcc atgaccatga tttgcccctg
    16441 tcgaggctga gcgcgggacc gcgggtgccg atgggattcg agcgcgaaaa gctgaactgc
    16501 aacgtccgcc ccgccccgcc gcggacccga aaataccagt aaaaccaatc accctccgta
    16561 tcgcgcaagt ccgggcgcaa cgtcacgagg tcgcccgcga tggaatccac cacaatattg
    16621 ccaccaggaa agttggcatc cacgcgaatc tgcgttctgg ccgctgcgcc gccttcggct
    16681 gcccgcgtga tgaagttcgc gccgagccat aggcagctca cggccaccat gatccccagc
    16741 cggccaataa cgaaacgcgt catattgaag ctgctatgga gagtgccggc tgtcatattc
    16801 cctcgccagc caaggcttgg ccggcaccgg ggatatcggc agcccagcct cgcgggcaat
    16861 cccgttccgc ggcgacccaa ccagggcgcg ccgccgaccc gcaaccggcg cgcatttcgc
    16921 cgcgcttccc gcctgtgatt tcatgggcgc agcctagccc agttcttttg gcttgtcccg
    16981 gattttatgc tatattcctg catgagatta gggttttgca taatctcatg gtggcctgcc
    17041 cgggtgcgga ttggcggacc cgcgccaatc ctcgcgcgca ccgggtattc cggcctgtac
    17101 cacgcggttt gcctgcgcag ggcccaaggc aaaacgggag gttccgctag atgaaccacc
    17161 aactgcacat cggctttgag actcggcctg accggcgaac ggcgcgctgc atccggaaac
    17221 gttcccatcg cgcccagtgg tggttcgagc ggatgcgcga gattgtggcg gaggcccaag
    17281 actggccgcc cgtcgctccg ccgtccgccg cagacgaagc ctccgtcgcc ccgatggcct
    17341 ccggcgagcc ttccgatcac acctgacgcc tgaagaccac gcccggccgc ccggtgggtt
    17401 tccgcgcgca tgatccctcc gactctgccg ggcgcccggc caaggtcatc atggcccggc
    17461 ccgaacgcac gaagagcatc ggtgcgaaca gcccccgccg cctccccggc agtctaatat
    17521 aactgcgtcg cctcgtcctt ctggatgggc ttctcgaatt ggtcgctgcg gatgaagacc
    17581 ttaaagcgtg cgtcgtccgg tttcagagct ttgaccgtga cctgccaaac cgcctgcgcc
    17641 ttgggcgcca gcacgggcac ggtttccatc gtcaccgtgc ccccctggcc ctgcacggct
    17701 gtggggccgg tgccggagac gaactcctcc gactccggca aggcgcaggt gaccttgaga
    17761 ttggttccgg gagcggaacc ctggttggtc actttaattt cataaacgac ctctttgccc
    17821 acctcaatcg ggtcctccaa atcggcggtc tccagcagaa tggccgggat gccggcgacg
    17881 agggtttcgc aacgggtgct caccgccggg ccgctggcgc cttccacggt cgccgcaaag
    17941 gcggccgtgg cggcctggtg cgccacaaaa gtggcgcaga cttgtttggc ggtgcccggc
    18001 tgcagcgcgg gcagcttcca cactatattt ccgtcggcgg gatctccctg gtcggtgacg
    18061 ccggccagaa ccgcttcggg aggcaccggg agccgcagcg tgggctgcgg ttcgagcgcg
    18121 gttcccaggt tggtcaccgt gaggcaaacc tcgaagcgcc gccccaccaa ggcctggccc
    18181 ggggcgctgc acccgagcgc caactccggc agcgccggcc cgaggtccac cgtgtagaaa
    18241 ttgctgcacg gccggggagt gatgcagcgg tagcgccgtc ccttcgaggt gaaatcgaag
    18301 gcatacgcgc taatcggctc cgtcccatcc cacaggacat ctatgagcgc ctccggctcg
    18361 ccgttcttgc gcgacgacat gtagggcatc cgggtgccaa ccggcaggct cagttcggta
    18421 atcggcgcaa ccgccgccgc ctgccgcaaa tcctccacct cgccttccca tcccgcctgc
    18481 cgcaggattt ccgccacatc agccttgagt ttctcatccg caaaaagcgc ccgcaactgc
    18541 tccggccgtt cgagcggcgg ggcgaaccgg gttaacggat gacccaaccg cacggcgcgg
    18601 tgctgctggg cttgcgccgc ggccaccgcc agcaacagcg tgaccacagc caggacagta
    18661 aggctggtac tattcttcat acgaaataca atcagcgcgg cgccgcggcg cgggctgcaa
    18721 gccagcccgg caattaagca tgcctctcat gctggttgca cggcccaacc actggcgcgc
    18781 cgcccgggct ttcccggacg gcgcgtgatt cccgcgcgag atcgcgccgg
//
LOCUS       DAOPQG010000073        18596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3607_length_18596_cov_15.181651, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000073 DAOPQG010000000
VERSION     DAOPQG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 18596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18596
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3607_length_18596_cov_15.181651"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..1705)
                     /locus_tag="PKI07_09330"
     CDS             complement(410..1705)
                     /locus_tag="PKI07_09330"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51293.1"
                     /translation="MTASASKSSSVNRREFLRCSAAALALGFPMIVAPGALGAPRRPS
                     PNDRLRVGIIGMGGRARWILKHEALPGADVIAVADCHLGRIREAAQQVEGGDRWRVYQ
                     QYREMLEKERLDAVFVETTTHARVLICIHAMQAGCDTYVEKPQSLTLAEGRVLVKAAR
                     KYKRIVQTGSQQRSMPINIHASRLVREGAIGKVHTVITCNFAPGRVWQPRDTPDDLPA
                     ELDWNQWCNQTELLPFYLELYNSWGEYVQYDGGGQCWGVTGWGTHSLDQVQCALGTDN
                     TGPVEIWPEEPGPRCKLTMRYANGVQVKLEGANRDLEDLGAIFIGDKGRIEILRGDYT
                     ADPPELRQHAPPVTPQGPNESVPHIENFFNCMRTRKPTNADAETGHRATTLCHLVNIC
                     RTVQRKLRWDPRKEEFIGDAEANQLRSRPRRKGFELPKI"
     gene            complement(1743..2522)
                     /locus_tag="PKI07_09335"
     CDS             complement(1743..2522)
                     /locus_tag="PKI07_09335"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC51294.1"
                     /translation="MQFCHRFIGAVLLLAAALSALGQAGKASGRFVSIFNGKDLSGWE
                     GLPGAWHVEDGAITAESTPEKPCTRAHYLMWRGGLPGDFELRLDFRLQGPGGNSGIQF
                     RSRELPDWDTSGYQADIEYGEQWTGCLFEHTRGGVVMRGEKAYIDRDGRKTITPIAAS
                     AGVRKLMAGKGAAGKGLSLSAALLRQVKMGEWNTYAIIARGPDITLKINNVVMCQVTD
                     HQAGLAVREGVLALQMHPGPPMKIQFKNLRLKQFRPRSDYE"
     gene            2665..4044
                     /locus_tag="PKI07_09340"
     CDS             2665..4044
                     /locus_tag="PKI07_09340"
                     /inference="COORDINATES: protein motif:HMM:NF021997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOC51295.1"
                     /translation="MPTDMLALNPIQPPSRRQFLRTSAVTALAAALPGSASARASSRR
                     GTVRDRLWIWAHDGQFYNCEFGLPRKSRITPVEGAFYMGVPNVMFIQYQGVPVPPFEQ
                     YYVPFKALRRVFWTLSNNANQMHRLGAEQEHVYRLAAANPNIQGLLLDDYLIGPLQPA
                     AGRQWLAENSPAFPVTLTLRLAAPARANTLELQQTDWESGDYRTARLEVDVAAREDAW
                     APVGRFELPNEPGARLRVPLPPGALAAVRVQILSTHDPQGARSCGLNELRLLADGQPL
                     ALDGAQIHASSQFPGHEAFHLLDNRAAPGQSPGYAAQVGLDDLAAARRRMAGLGRPLE
                     LAAVVYAHQLDPAIAPLLAPIDTVLFWTWRAAELPGLEANFRRLKQLLPGKRVLLGCY
                     MWNFGEPNGPMPLAQLARQCDLGLRWLQAGEIEGLIFLGTNICDLDLEAVEWTRRWIA
                     THGDKPLKR"
     gene            4168..4611
                     /locus_tag="PKI07_09345"
     CDS             4168..4611
                     /locus_tag="PKI07_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOC51296.1"
                     /translation="MKITEALLAEHLVFHNMFDHVEAVAPKLKTLAEVKSLAALLETL
                     LKAHSDTEDELFIGPLEHCFEQIGQRDAFLEEHQELDACLVRIRRVRQLKKARQLLVA
                     AVAYSREHFDKEERIVFPLAERVLSHKTLTALGDTWAEQRTRSLP"
     gene            complement(4629..5708)
                     /gene="bioB"
                     /locus_tag="PKI07_09350"
     CDS             complement(4629..5708)
                     /gene="bioB"
                     /locus_tag="PKI07_09350"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOC51297.1"
                     /translation="MSVTLIDAAAHRRIAELGQRVLAGGGVTRAEGEWLFALARPEDR
                     LDLLVWANRIREHFHGRRVRLCSIVNVKAGGCPEDCRFCAQSAVHAADSPRHALLEAD
                     EVREAAREAQAQGVAALGLVAAWRDLREGPALDQMCARLTELKQSGQVRPDASLGMIR
                     SQKVADRLKAAGCECYNHNLETSRRFFSQVCTTHTYEDRVRTLHYLKQAGIRICSGGI
                     LGMGETREDRCDLAFALRELGAHTVPLNFLNPIAGTPFAGLTPLAPMEILQIIACFRF
                     ILPQSEITIAGGRAVNLRDLQSWIFMAGASALMVGNYLTTANQPVAKDLQMLKDLGLS
                     WRGDEPGGPRAVPASAAAPPVPAAA"
     gene            complement(5791..6033)
                     /locus_tag="PKI07_09355"
     CDS             complement(5791..6033)
                     /locus_tag="PKI07_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51298.1"
                     /translation="MRILLQRIASGLYYEDTDAWTPDSFGAMDFLSSTAALEFCAANK
                     LTGVQIVLKFDEEKREIVLPILPLPPGQNQRPTASL"
     gene            complement(6228..6758)
                     /gene="lepB"
                     /locus_tag="PKI07_09360"
     CDS             complement(6228..6758)
                     /gene="lepB"
                     /locus_tag="PKI07_09360"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOC51299.1"
                     /translation="MLLPRSRPAQSRGRAFFQQVFQYLVVAALAGGCYFAISHFFVQS
                     VRVVGRSMVPTLQDSQRYLLNRWLYRVRPPQRSDIVVVRDPGDNGFSVKRIIAVPGDS
                     IYLKDGGVYLNGSKLKEPYLAPGTPTFPELKPQDQLILCGKDQYFLLGDNRANSVDSR
                     RYGPVPRGNILGPIMR"
     gene            complement(7020..11162)
                     /gene="rpoC"
                     /locus_tag="PKI07_09365"
     CDS             complement(7020..11162)
                     /gene="rpoC"
                     /locus_tag="PKI07_09365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOC51300.1"
                     /translation="MISTKESARELLGLEKVNQVEHVAISVASPDAIRSWSKGEVKNP
                     ETINYRTFKPEKGGLFCERIFGPVKDWECSCGKFKRIKHRGVICDRCGVEVTLARVRR
                     ERMGHIELAVPVCHIWFFKCMPSRIGLVLDMTARNLERVIYYEDYVVIDPGSTPLKQN
                     QLLSEHEYHEAIETYGAEAFVAKMGAEAVREALAKVDLGKQITQLQAAMGETKSKQIR
                     KKIAKRIKLLQGLQASKSRPEWMILTVLPVIPPDLRPLVPLEGGRFATSDLNDLYRRV
                     INRNNRLKNLLQLKTPEVIIRNEKRMLQEAVDALFDNGRHGRAVTGAGNRALKSLSDM
                     LKGKSGRFRQNLLGKRVDYSGRSVIVIGPELKLHQCGLPKKMALVLFEPFIIRRLKEL
                     GYVHTVRSAKKLIERQTTEVWDILDEVTRGHPVLLNRAPTLHRLSVQAFEPQLIEGEA
                     IRIHPLVCTAYNADFDGDQMAVHVPLSVEAQLEARLLMMAPNNMFSPSSGRPIITPTQ
                     DITLGCYYLTAEPRTPAPADARQLKLFGSKTEVAFAFNDGAVKTHERIRLANPDYGKQ
                     TAFGDASKKVIETTVGRVFFSEIWPPELGFPNKAVKKNELGDLIWRCYKICGHDKTVT
                     MLDKLKELGFREATHAGVSIGIDDMIIPKEKDQEIDSAQKQIREVEKQYRRGIITPGE
                     RYNKIIDIWTHCTDQIANVMLRTLEHNQGKGEYNPVSLMVGSGARGNRQQVRQLAGVR
                     GLMAKPSGDIIEKPILSNFREGLTVLEYFISTHGARKGLADTALKTADSGYMTRKLVD
                     VAQDVIIREEDCGTTNGIWVQAIYEGEDEVVKLSERLAGRFACDDHPNPQQPKELLVK
                     ANEEIDEVKAQQIEAAGVERVKIRSVLTCESKHGVCVYCYGRNLATGVRVKLGEAAGI
                     IAAQSIGEPGTQLTMRTFHIGGTASQVFKVPQIKAKHDGILRYNELRLVTLEDGSNIV
                     LNKNGSVSILDNDGRELEQHPVVIGAVISVGDGGKVKKGETFVQWDPYNVPILSEKAG
                     RVKFHDIIEGVTMKQETDETTGQEAMVIIEHKEDLHPQVIICDDRGEPVANYLIPSGA
                     HVVVAEGDKIVAGTLMAKTPRKTSKTRDITGGLPRVAELFEARRPKDAAEISKIEGIV
                     DFGPSVRGKRCIIIKDLQTGVQEEHLIPLGKHVIVFKGDYVKKGQQLTEGPVDPHEIL
                     DICGPQELQEHLVNEVQEVYRLQGVTINDKHVEIIVRQMLRKVRITEPGDTGFLWGEQ
                     VDKLEFEEENSRVEKMGGQAAEAQPVLLGITKASLETESFLSAASFQDTTRVLTEAAT
                     RAKVDYLRGFKENVIMGHIIPAGTGFDYHRKARLKPLVEISEDEPDLLKALAADENPL
                     LG"
     gene            complement(11213..15073)
                     /gene="rpoB"
                     /locus_tag="PKI07_09370"
     CDS             complement(11213..15073)
                     /gene="rpoB"
                     /locus_tag="PKI07_09370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOC51301.1"
                     /translation="MSTRASQRINSGKIKEIIAPPNLIELQTNSYRDFLQAGVAPSKR
                     ENLGLQAVFTEVFPIESYDGKCVLDFHSYEIGEPKVDWLECLREGLTYGAPLYVTFQL
                     KEERTVKEEKVFMGELPLMTPQGTFVINGAERVIVSQLHRSPGLAFEATLHPNGKTLH
                     SFRIIPDRGSWYEAQFDTSDLLYVYLDRKKRRRKFLTTTLFRALAFLDGEGRGKDNQD
                     KNRGTDEEILKLFYEIEEFSVKEAEKRDDLANKVLIEDAVDQAEGLVIARAFEPLSKA
                     VVRQIAELGVSKIRVVDTTQDDGIIIKCMKKDPAKNEEEGLKDIYRRLRPGDPPTTAN
                     ARALIKRLFFDPKRYDLGRVGRYKINQKLGIKGGDSRILTKADLVAATKYLLRLKKGE
                     GSLDDIDHLGSRRVRTVGELLANQCRVGLARTERLVKERMTLFDQGVEAMSPQKLINP
                     KALSAVIRDFFGRSQLSQFMDQINPLAELTHKRRLSALGPGGLSRDRAGFEVRDVHPS
                     HYGRICPVETPEGPNIGLIASLATFARINDFGFLETPYRKVEGGRVTNQLDYLTADRE
                     ESFVIAQANTPIEDKGKFTTDRVVCRGKGDFIDAEPSRVDYMDVSPKQLVSVAASLIP
                     FLEHDDANRALMGSNMQRQAVPLLVTEAPLVATGLEERVARDSRAVIVAEAAGKVAAV
                     PGGQIIITENGKLPEGKKKIKHDPDKGVFVYPVRKFMRSNAATCINQKILVKKGETVK
                     KGQVIADGPCTQGGELALGRNVLVAFMPWNGYNFEDAILVSERIVKEDIFTSIHIDEF
                     EVGARDTKLGPEEITRDIPNLGEEALRNLGPDGVIRLGAEVKPGDILVGKITPKSETE
                     LAPEERLLRAIFGEKAADVKDTSLKVPSGTYGIVMDVKVSAKKESDRDAKTSANEEKR
                     HAREVEEEYRRKTEELREQLTEALSNILLGEKIPLDVVNSETGEVIIPANRKITKTLL
                     RKLAQVYDRIEIDPSPIRNKINEIIGSFKKKFDDLQMQYDEEIERAESGEGVDSGVIK
                     SVKVYIASKRKLSVGDKMAGRHGNKGVVAKIMSEEDMPFLADGTPVDIVLNPLGVPSR
                     MNVGQLLETHLGWAAKLLGLHFATPVFDGIKEKDIRSYLKQAKLPEHGKARLFDGRTG
                     IPFDQEIVVGYIYMMKLGHLVADKIHARAVGPYSLVTQQPLGGKAQYGGQRFGEMEVW
                     AMEAYGAAYTLQELLTVKSDDVQGRTRIYENIVKGDNSLEAGIPESFNVLVKEMQSLG
                     LDVKVGYSNPLDHASEPIRALTAV"
     gene            complement(15296..15685)
                     /gene="rplL"
                     /locus_tag="PKI07_09375"
     CDS             complement(15296..15685)
                     /gene="rplL"
                     /locus_tag="PKI07_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOC51302.1"
                     /translation="MALDKAKIVEELSNATVIEIADLVKELETKWGVSAAAPVAVAAA
                     APGAGAAAPAAEAQTQFDVILTAVAADKKISVIKAVREVKAGLGLAEAKALVEGAPKT
                     VLEGASKAEADAAKKKLEDAGAKVELK"
     gene            complement(15827..16363)
                     /gene="rplJ"
                     /locus_tag="PKI07_09380"
     CDS             complement(15827..16363)
                     /gene="rplJ"
                     /locus_tag="PKI07_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOC51303.1"
                     /translation="MRPEKQNLTKEYLARLNASPFFIVVNYKGLKVVQLIELRKRLSA
                     AGAEIHVVKNSVFREAAREAGVGDLNGSLSGQLAVVTGQRDISTAAKVVKTFGTELDR
                     LKVQFGYLNNQRLEQPEIMMLADLPSLEVLRAKLLALFNAPATTLVRLLNTPAEQMAR
                     ILQARADQQAEAAKPAAN"
     gene            complement(16405..17097)
                     /gene="rplA"
                     /locus_tag="PKI07_09385"
     CDS             complement(16405..17097)
                     /gene="rplA"
                     /locus_tag="PKI07_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOC51304.1"
                     /translation="MPSKRYRKALELVDVRKTYPLKDAVEVLAKFPKAKFNETVELAF
                     CLGVDPKQSDQMVRGTVPLPHGSGKTVRVLVFAKSGPAAEAAKSAGAEYVGFEDMLKK
                     CQEGWTDFDVAVATPEAMGEVRKLGKVLGPRGLMPNPKTGTVTEDTARAVKEVKAGRV
                     EFKVDKAGNIHVPVGKLAFAATQIEENVRAVIDAVAKARPQSVKGTYIKTCTLSGTMS
                     PPVRLDLKEFAV"
     gene            complement(17168..17593)
                     /gene="rplK"
                     /locus_tag="PKI07_09390"
     CDS             complement(17168..17593)
                     /gene="rplK"
                     /locus_tag="PKI07_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOC51305.1"
                     /translation="MAKKITGVIKLQIPAGQANPAPPVGPALGQHGVNIMAFCKEYNA
                     KTRDQTGTIIPVVITVYQDKSFTFITKSPPAAVLLKKAAGITAGSKEPNKEKVGKVTR
                     KQVLDIVKIKLKDMNATSEEAAFRVVAGTARSMGIEVVG"
     gene            complement(17673..18221)
                     /gene="nusG"
                     /locus_tag="PKI07_09395"
     CDS             complement(17673..18221)
                     /gene="nusG"
                     /locus_tag="PKI07_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOC51306.1"
                     /translation="MRSQWFIIHTLAGQEQKVKESIEKRLKAEEMGDYIKEVLVPMEK
                     VVEVRNQKKTVSSRKLWPGYVFVDMVLLDENKQIVEKPWYFIQDTPGVIGFVGGEPPA
                     PTPAEEVEAIKKQISASEEYEKPKVSFEVGETVKINNGPFLNFSGLIEEIEPERGKLK
                     VTVNIFGRNTPVELEYWQVEKG"
     gene            complement(18226..18477)
                     /gene="secE"
                     /locus_tag="PKI07_09400"
     CDS             complement(18226..18477)
                     /gene="secE"
                     /locus_tag="PKI07_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOC51307.1"
                     /translation="MITLLIWAVIIGAAFAYCWRKGYLLQLTNYVQQTREELRKCTWP
                     TWGELKGSTVVITISIVLLGTFIVLVDQVFFRLFMFFKL"
     gene            complement(18507..18596)
                     /locus_tag="PKI07_09405"
     tRNA            complement(18507..18596)
                     /locus_tag="PKI07_09405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18560..18562),aa:Ser,seq:tga)
BASE COUNT         3411 a         5853 c         5407 g         3925 t
ORIGIN      
        1 ggccgaactg gatccacagc gccatcgtcc cgccctcgtc cacggccaaa tctcatcaag
       61 ccactgaaaa ggcgccagtt aaaaaagcga agtccgcgtc cgcctcgccc tcttccaccg
      121 ctcgcacgac cgttctttct tcgtcgtctc aggctatgta caaggggagt acaccggagg
      181 cgcggcagga gcgcatggaa aataagcatg aacccccttt gagcatcctt ggtacacccc
      241 ttgtacacgg cctattggga gccataggct ggctggggaa gctgggaagg gggtgggcgc
      301 gcgcctgagg cggaattgtg gcccttattg gccgaaaggg gctagcccct tttcgggatg
      361 acctgcggtt gcccggttgg gctttgcgcc tggctgccgg ctggggttat cagattttag
      421 gcaactcgaa gcctttgcga cgagggcgcg accgtaattg gtttgcctcg gcgtcgccga
      481 tgaattcctc ttttcgaggg tcccaacgga gcttgcgctg gaccgtgcgg cagatattga
      541 cgagatggca gagcgtagtg gcgcggtgcc cggtttcggc gtcggcgttg gttggcttgc
      601 gcgtgcgcat gcagttaaag aagttctcga tgtgggggac cgactcattg ggtccttgcg
      661 gcgtcaccgg gggcgcgtgc tggcgcagtt cgggcggatc ggcggtgtaa tcgccgcgga
      721 gaatctcgat gcggcctttg tccccgataa agatcgcgcc gaggtcttcc aggtcgcggt
      781 tggcgccctc cagtttgacc tggacgccat tggcatagcg catggtgagt ttgcagcgcg
      841 gccccggttc ttccggccag atttcgaccg ggccggtgtt atcggtgccg agggcgcact
      901 gaacctggtc gaggctgtgg gtgccccagc cggtgacgcc ccaacactgg ccgccgccgt
      961 cgtattggac gtattcgccc cagctattgt acaactccag gtagaagggg aggagttcgg
     1021 tctgattgca ccactggttc caatccagtt ccgcgggaag gtcgtcgggg gtatcgcgcg
     1081 gttgccagac gcggccgggg gcgaagttgc aggtgatgac ggtgtggact ttgccgatgg
     1141 cgccctcgcg cacgagccgg ctggcgtgga tgttgatggg catcgaacgc tgctgggagc
     1201 cggtctggac aatgcgcttg tatttgcggg cggctttgac caggacgcgg ccttcggcca
     1261 gcgtaaggct ctggggtttt tccacgtagg tatcacagcc ggcctgcatc gcgtggatgc
     1321 agatcaacac gcgggcatgg gtggtcgttt ccacaaacac cgcgtcgagc cgctcctttt
     1381 ccagcatttc gcggtattgc tgatagacgc gccagcggtc gcccccctcc acttgctggg
     1441 cggcttcgcg gatgcggccc aggtggcaat cggccacggc gatgacatca gcgccgggca
     1501 gggcttcgtg cttaaggatc cagcgggcgc gcccgcccat gccgataatg ccaacccgga
     1561 gccggtcgtt ggggctgggg cggcgcggcg cgccgagggc tcccggcgcc acaatcatcg
     1621 gaaaacccag ggccagggcg gcggccgaac agcgcaggaa ttcgcgccgg ttcaccgaag
     1681 aggactttga agcagaggca gtcatagctg agacggattt ggtttcagga acgaaggatt
     1741 gattattcat agtcggagcg ggggcgaaat tgtttgaggc ggaggttttt gaactggatc
     1801 ttcatcggcg gccccggatg catctggagg gccagcacgc cctcccggac ggccaggccc
     1861 gcctggtgat ccgtcacctg gcacatgacc acattgttaa tcttcaaggt aatatccggg
     1921 ccgcgcgcga taatggcata ggtgttccac tcgcccatct taacctgccg gagcaacgcg
     1981 gcgctgaggg aaagcccttt ccccgcggcg cccttgccgg ccatgagttt gcgcacgccg
     2041 gcagaggcgg cgatcggcgt gatggttttt cgcccgtccc ggtcaatgta ggccttctcg
     2101 ccgcgcatta cgacgccgcc gcgggtatgc tcgaagaggc agccggtcca ttgttcgcca
     2161 tattcgatgt cggcctggta gcccgaggta tcccaatcgg gcagttcacg gctgcggaac
     2221 tggatgccgg agttgccgcc cgggccctgg aggcgaaagt ccaggcgcag ctcgaaatcg
     2281 cccggcaggc cgccgcgcca catcaggtag tgggcgcggg tgcagggctt ttccggggtg
     2341 ctctccgccg tgatcgcgcc gtcctcgacg tgccaggccc ccggcaagcc ttcccagccg
     2401 ctcaagtcct ttccgttgaa aatggagacg aaccgccccg aggccttccc cgcctgaccc
     2461 aaggccgaaa gcgccgccgc gagcaagagc actgcgccaa taaagcggtg gcaaaactgc
     2521 atacgccaga aactaaggcg cgacttcgcg aagtcaaccc cggttcttca gaaagttaaa
     2581 tcgcgacgcg gacgcccgcc cctggcgggc cgggccggcg cggcgggcgg cagaataact
     2641 ttgccaggcg agacgatccg ggttatgcca acggatatgc ttgcgttgaa cccgattcag
     2701 ccgccctccc gccgccaatt tctccgcacg agcgccgtca ccgcgctcgc cgccgccctg
     2761 cctggaagcg cgtcggcccg cgcttcgagc cggcgcggca ccgtgcgcga ccggctgtgg
     2821 atttgggcgc atgacgggca gttctacaac tgcgaatttg ggctgccccg caagagccgc
     2881 atcacgcccg tggaaggggc gttttacatg ggcgtgccca acgtgatgtt catccaatac
     2941 cagggggtgc ccgtgccgcc tttcgagcag tattacgtgc ccttcaaggc gctgcggcgg
     3001 gttttttgga cgctgtccaa caatgccaac caaatgcacc ggctgggggc cgagcaggag
     3061 catgtctatc ggctggccgc cgcgaacccg aacatccagg gtctgctgct ggatgattac
     3121 ctgatcgggc ccctccagcc ggcggcgggc cgccaatggc tggcggaaaa cagccccgcc
     3181 tttccggtga ccctgaccct gcggctggcg gcgccggccc gggccaacac gctggaattg
     3241 cagcagacgg actgggaaag cggcgattac cgcaccgcgc gcctggaggt ggacgtggcc
     3301 gcccgggagg atgcctgggc gccggttggg cgcttcgaac tgcccaatga accgggggcg
     3361 cggctgcggg tgccgctgcc cccgggcgct ctggcggcgg tgcgcgtgca gattctctcg
     3421 acccatgacc cgcaaggcgc ccggagttgc ggcttaaacg agcttcgcct gctggcggac
     3481 ggccagccgc tcgcgctgga cggcgcgcaa attcatgcga gcagccagtt tcctggccat
     3541 gaagcctttc acctgctgga caaccgcgcg gcgcccgggc aatcccccgg ctacgccgcc
     3601 caggtcggtt tggacgattt ggcggcggcg cggcggcgaa tggccggcct gggccgtccg
     3661 ctcgagctgg cagcggttgt ctatgcccac cagcttgacc cggccattgc acccctgctg
     3721 gcgccgattg acacggtgct gttctggacc tggcgggcgg cggagcttcc gggcctcgag
     3781 gcgaatttcc ggcggctgaa gcaactgctg ccgggcaagc gggtgctgct ggggtgctat
     3841 atgtggaact tcggcgagcc gaacggcccg atgccgctgg cgcagcttgc ccgccagtgc
     3901 gacctgggcc tgcgctggct gcaagccggg gagatcgagg ggctcatctt tctgggcacc
     3961 aatatctgcg atctggacct tgaggccgtc gagtggacgc gccgctggat cgcaacgcac
     4021 ggcgacaaac cactcaagcg ctgaccgttt tgcgccgaag cggccggcgc cgttcccggg
     4081 cggccgcgcg gcggcgagaa taaaagagtg gcgcgcgggc ctgttttctg ctaagcgagt
     4141 acgcgcagcg ataaactaac tgccacaatg aaaatcaccg aagctttgct ggccgaacac
     4201 ctggtgttcc acaacatgtt tgatcacgtc gaggcggtgg cgcccaagct caagacgctg
     4261 gccgaggtca agtcgctggc ggcgttgctg gagacgttgc taaaggcgca ttccgacacg
     4321 gaggacgagt tgtttatcgg gccgctggag cattgtttcg agcagatcgg gcaacgggac
     4381 gcgtttctcg aagaacacca ggaactggac gcctgcctgg tgcgcatccg gcgcgtccgg
     4441 cagctcaaga aggcgcggca actgctcgtg gcggcggtgg cctactcgcg cgagcacttc
     4501 gacaaggaag aacgcatcgt cttcccgctg gccgagcgcg tgctgagcca caagacgctc
     4561 accgccctgg gcgatacctg ggcggaacag cgcacgcgct ccctcccgta agacggcggc
     4621 ctttccggct acgcggcggc cggcaccggc ggcgcggcgg cggacgcggg aacggcgcgg
     4681 gggccgccgg gctcatcccc gcgccagctg aggcccaggt ctttgagcat ttggaggtcc
     4741 ttcgcgacgg gttgattggc cgtggtgagg tagttgccga ccatgagggc gctggcgccg
     4801 gccatgaaga tccagctttg caggtcgcgc aagttcaccg cgcgccctcc ggcgatggtg
     4861 atttccgact gcggcaggat gaagcggaag caggcgatga tctggagaat ctccatcggg
     4921 gccaggggcg tcaacccggc gaacggcgtg ccggcgatgg gattgaggaa gttcaggggc
     4981 accgtgtgcg cgcccagttc ccgcagcgcg aaggccaggt cgcagcggtc ctcccgggtt
     5041 tcccccatgc ccaggatgcc gccggaacaa atgcggatgc ccgcctgctt taaatagtga
     5101 agggtgcgca cgcggtcctc gtaggtgtgg gtcgtgcaga cttgcgaaaa aaagcggcgc
     5161 gaggtctcca ggttatggtt gtagcactcg caacccgccg ctttgaggcg gtcggccact
     5221 ttttggctgc gaatcattcc cagcgaggca tccggccgca cctggccgga ctgcttcagc
     5281 tccgtcagcc gcgcgcacat ctggtcgagc gcagggcctt cccgcaaatc gcgccacgcc
     5341 gccaccaacc cgagcgccgc cacgccctga gcctgggctt cccgggccgc ttcccgcacc
     5401 tcgtccgcct ccagcagggc gtgccgcggt gagtccgcgg cgtgaaccgc ggactgggcg
     5461 caaaaacggc agtcctccgg gcagccgccg gctttcacat tgacgatcga acacaggcgc
     5521 acccggcggc cgtgaaagtg ctcgcgaata cggttcgccc agaccagcag atcgagccga
     5581 tcctccgggc gcgccagcgc aaacagccac tcgccctccg cccgggtgac gccaccaccg
     5641 gccaggactc gctggcccag ctcggcgatg cggcgatgcg cggccgcgtc aatcagagtc
     5701 acgctcatgg ttgacagcct agtcaccggc gcacagcggc gcaagcggcg agtggcgcgg
     5761 acattttgcg caggggttga agcgcgcgga ctaaagcgaa gctgtcgggc gctggttttg
     5821 cccgggcggc agcggcagta tcggcaagac gatctcccgc ttctcctcgt cgaatttgag
     5881 cacgatctgc actcccgtca gtttgtttgc cgcacaaaac tccagggcgg cggtggagct
     5941 gagaaaatcc atcgccccga aactgtccgg cgtccacgcg tccgtgtctt catagtaaag
     6001 accgctggcg attcgctgca gaagtattcg catacgcaga attcagtgtt gtcttcaccg
     6061 ttgcggacac cttcgccggt cgcgcgggaa cgcgctcgcc ttcccacgcg gcaatcggcc
     6121 gagtctgcgt tgggtatttt gcgcccggcg tgatttccgg caagcctaaa atcagggcgc
     6181 cggacccgcc gccggccggc tgtgggggcg ggcaattccg cgccgggtca gcgcatgatg
     6241 gggcctaaaa tgttgccgcg cgggaccggc ccatatctgc ggctgtcaac cgagttggcc
     6301 cggttatcgc ccagaaggaa atactggtct ttgccgcaca ggatcagttg gtcctgcggc
     6361 tttaactcgg ggaacgtcgg cgttcccgga gcgaggtagg gttccttgag tttgctcccg
     6421 ttgagataca cccccccgtc cttcaggtag atcgaatctc cggggacggc gatgatgcgt
     6481 ttgacggaga agccgttatc gccggggtcg cgcacaacca caatgtcgct gcgctgcggc
     6541 gggcggacgc gataaagcca gcgattcagc aggtagcgtt gcgagtcctg gagcgtgggg
     6601 accatgctcc gtcccaccac gcggaccgac tgaacgaaga agtggctgat ggcgaagtag
     6661 catccgccgg caagcgccgc gaccaccagg tattgaaaga cctgctggaa aaatgcgcgc
     6721 ccgcgtgatt gggccgggcg cgaacgcggc agcagcactg atccgtttaa taccggtctc
     6781 gaatccgttg tgtgcgtcac cacgctcatc tatgccctct caatttatcg cgcgggtgcg
     6841 gggccacaag tcgtgaaaaa tacgcagggc cgttgtcggg tcaatcccgg gccgcggcgg
     6901 cccgggattc gccggccccg ccgtgtcctt ttgtatggcc tcgggaacgt gcccatcggg
     6961 ctcaggttcg ccgatttgcg cgtcagcccg atcgccgcgc gcccatcccg ccgggccggt
     7021 cagcccagca acggattctc gtcggcagcc agagctttca gcagatccgg ttcatcctcg
     7081 ctgatttcca cgagcggctt gagccgggct ttgcgatggt aatcaaagcc cgtgccggcg
     7141 ggaatgatat gccccatgat gacgttttcc ttgaagccgc gcaaataatc aaccttggcg
     7201 cgcgtggctg cctccgtcag cacccgggtg gtgtcctgga aggatgcggc gctcaggaaa
     7261 ctttctgttt ccagcgaggc tttggtaatg ccgagcagga cgggctgggc ctcggcggcc
     7321 tggccgccca tcttctcgac gcgcgaattt tcctcttcga actcgagctt gtccacctgc
     7381 tcgccccaga ggaacccggt gtcgcccggc tcggtgatgc gcactttgcg cagcatctgg
     7441 cggacgataa tttcaacgtg cttgtcgttg atcgtcacgc cctgcaaccg gtaaacttcc
     7501 tggacctcat tgaccaggtg ctcctggagt tcctggggac cacagatgtc caggatttca
     7561 tgggggtcca ccggaccttc ggtaagctgc tggcctttct ttacgtaatc gcccttgaac
     7621 acaatcacat gtttgccgag cggaataaga tgctcctcct gcaccccggt ttgcaggtct
     7681 ttgatgatga tgcaacgctt gccgcgaacg ctgggaccga agtccacaat gccctcaatc
     7741 ttggaaatct cggcggcatc tttcggccgc cgggcttcaa agagctcggc cacgcgcggc
     7801 aatccgcccg tgatgtcccg ggtcttcgag gtcttgcgcg gggtcttggc catcaaggtg
     7861 cccgcgacga tcttgtcgcc ttccgcgacg acaacgtggg ccccggaagg aatcagataa
     7921 ttagcgaccg gctcgccccg gtcatcgcaa ataatgactt gcgggtgcaa atcctccttg
     7981 tgctcgataa tcaccatggc ctcctggccg gtggtttcgt ccgtttcctg cttcattgtg
     8041 accccctcga tgatgtcatg gaatttgacg cggccggctt tctccgaaag aatcggcacg
     8101 ttgtagggat cccactggac gaaggtctcg cccttcttga ccttgccccc gtccccgacc
     8161 gaaatgaccg ccccgatcac cacggggtgc tgttccaact cgcgtccgtc gttatcaagg
     8221 atggagaccg agccattctt gttaaggaca atattgctgc cgtcttccag ggtcaccagg
     8281 cgcagctcgt tgtaacgcaa gatgccatca tgcttggctt tgatctgggg caccttgaaa
     8341 acctgggaag cggtgccgcc gatatggaac gtgcgcatgg tgagctgggt gcccggctcc
     8401 ccgatggatt gcgccgcgat aatgccggcc gcttcgccca gcttcacccg cacgcccgtg
     8461 gccaaattgc ggccgtagca atagacgcag accccatgct tgctttcgca agtcaacacc
     8521 gagcggattt taacccgttc cacgcctgcc gcctcaattt gctgggcctt gacttcgtca
     8581 atttcctcgt tcgccttgac gagcaattcc ttcggttgct gcgggttggg atgatcgtcg
     8641 caggcgaagc gcccggccag gcgttcgctt agtttaacga cctcgtcctc tccttcatag
     8701 atggcctgca cccaaatccc attggtcgtg ccacaatctt cctcgcggat aatcacgtcc
     8761 tgcgcgacat caaccagttt gcgggtcatg taccccgaat ccgccgtctt gagtgccgta
     8821 tcggccagcc ccttgcgggc gccgtgggtc gagatgaagt attcgagcac ggtaagcccc
     8881 tcgcggaagt tggagagaat cggcttctcg ataatatcgc ccgagggctt ggccatcaaa
     8941 ccgcgcaccc cggcaagctg gcggacctgc tgccggttgc cccgggcccc ggaacccacc
     9001 atgagcgaca ccgggttgta ttcccccttg ccctggttgt gctccagcgt ccgcagcatg
     9061 acgttggcga tctgatccgt gcagtgcgtc cagatgtcga tgatcttgtt gtagcgctcg
     9121 cccggagtta tgatgccgcg gcgatattgc ttctcgactt cccggatctg cttctgggca
     9181 ctgtcaatct cctgatcttt ttccttggga atgatcatgt cgtcaatgcc aatcgaaacc
     9241 ccggcatgcg tcgcttcgcg gaagcccagt tctttcagtt tgtccagcat ggtgaccgtc
     9301 ttgtcatggc cgcaaatctt gtagcaccgc cagatgaggt cgccgagttc gtttttcttg
     9361 acggccttgt tggggaagcc caattccggg ggccagattt cactgaagaa aacccggccc
     9421 acggtggtct caatcacctt cttgctggca tcgccaaagg cggtttgctt cccgtaatcc
     9481 gggttcgcca ggcgaatccg ctcgtgcgtc ttgaccgcgc catcgttaaa cgcgaaggcg
     9541 acttcggtct tggagccaaa cagctttagt tgccgcgcat ctgccggcgc cggagttcgc
     9601 ggctcggccg tcaggtagta gcagcccagg gtgatgtcct gcgtcggagt gataatcggg
     9661 cggccgctgg aggggctgaa catgttgttg ggcgccatca tgagcaggcg cgcttccaac
     9721 tgcgcttcga ccgacaacgg cacgtgcacg gccatctggt cgccgtcgaa gtccgcgtta
     9781 taggccgtgc aaaccagcgg atggatgcgg atcgcctcgc cttcgatgag ctgtggctca
     9841 aacgcctgga ccgacaaccg gtgcagggtc ggcgcgcgat tcagcagcac gggatggccg
     9901 cgcgtcactt catcgagaat atcccagact tccgtggttt ggcgttcaat caatttcttc
     9961 gccgaacgca cggtatgaac atagcccagt tccttgagcc ggcggatgat gaagggctca
    10021 aacaagacga gcgccatctt cttgggcaat ccgcactgat gcagcttgag ttccggcccg
    10081 atcacaatga cggagcggcc ggaatagtcc acccgcttgc ccagcaggtt ttggcgaaaa
    10141 cgcccgctct tgcctttgag catgtcgctc agcgacttca gcgcccgatt gcccgctccc
    10201 gttacggcac ggccatggcg cccattatca aacaatgcat ccacggcctc ctgcagcatg
    10261 cgtttctcgt tgcggatgat cacctccggg gttttgagct gcagcaggtt cttcaaccgg
    10321 ttattgcgat tgatgacgcg gcgatagagg tcgttgaggt cggaggtcgc aaaacgccct
    10381 ccttcgaggg gcaccaaggg gcgcaaatcc ggcggaatga cgggcagcac cgtgagaatc
    10441 atccattccg ggcggctctt ggaagcctgc aacccctgaa gcagcttgat gcgcttagca
    10501 atcttcttgc ggatttgctt gctcttggtc tcgcccatgg ccgcctggag ttgggtaatt
    10561 tgcttgccca ggtccacctt cgccagcgcc tcgcgcacgg cttccgcccc catcttggcg
    10621 acgaacgcct ccgcgccata cgtctcgatc gcctcgtggt attcgtgttc gctcagaagc
    10681 tggttttgct tgagcggcgt cgaacccggg tcaatgacca cgtagtcttc atagtaaatc
    10741 acccgctcga ggttgcgggc ggtcatatcg agcaccaggc caatccggga aggcatgcac
    10801 ttgaagaacc agatatggca gaccggcacc gccagctcga tatgccccat gcgttcgcgg
    10861 cgcacgcgcg cgagcgttac ctccaccccg cagcgatcac aaatcacgcc gcggtgtttg
    10921 atgcgcttga acttgccgca cgagcattcc caatctttga ccgggccgaa aatgcgctcg
    10981 caaaacaggc cgcctttttc cggcttgaag gtgcgataat tgatggtttc gggattcttg
    11041 acttcccctt tggaccagga ccggatggcg tcaggcgagg ctacgctaat cgccacgtgt
    11101 tcaacctggt tgaccttttc caaaccgagc aattcccggg cgctttcttt ggtgctaatc
    11161 atacattcga aatggcggtg atgacttggg gtggaaatac ttaaacgcgg ttctagacgg
    11221 cggtgagagc ccgaatgggt tcggacgcat gatccagcgg gttggaataa cccaccttca
    11281 catcgagccc cagcgattgc atttctttca cgagcacgtt gaaggattcc ggaatgcctg
    11341 cctccaggct gttatcgcct ttgacgatgt tttcgtatat tcgcgtgcgg ccctggacgt
    11401 cgtccgactt gaccgtcaac agctcctgca gggtgtaagc ggcgccatag gcttccatag
    11461 cccagacctc catttcgcca aaacgctggc cgccgtactg cgccttgccg ccgagcggct
    11521 gctgggtcac cagtgaatac gggcctactg cccgcgcatg tatcttgtcg gccacgaggt
    11581 gcccgagctt catcatgtag atgtatccca ccacaatctc ctggtcgaac gggatgcctg
    11641 tgcgcccgtc gaaaagccgg gctttgccat gctccggcag cttggcctgt ttcaggtaac
    11701 tgcggatatc cttttctttt atcccgtcga acaccggggt ggcaaaatgc agcccgagca
    11761 gcttggccgc ccagcccagg tgcgtttcca gcaattgccc gacattcatg cgggagggca
    11821 cgccgagggg gttcaacaca atatccaccg gcgtcccatc ggccaggaac ggcatatcct
    11881 cttccgacat gatcttggcg acgacccctt tattgccgtg gcggccggcc attttatccc
    11941 cgaccgaaag cttgcgtttc gaggcaatat agactttgac ggactttatg accccgctat
    12001 ccacgccttc gccggattca gcacgctcaa tttcttcatc gtactgcatc tgcaggtcgt
    12061 caaacttctt cttaaagctg ccgatgatct cattgatctt gttgcgaatc ggcgaggggt
    12121 caatttcgat acgatcataa acctgcgcca gtttgcgcag cagcgttttg gtgattttgc
    12181 gattggcggg aataatgact tccccggttt cgctgttgac aacgtcaagc ggtatcttct
    12241 cccccagcag aatattgctc agggcctcgg taagttgctc gcgtaactcc tcggtcttcc
    12301 gccggtattc ctcttcgact tcccgggcat ggcgcttctc ttcgttagcc gaagttttgg
    12361 catcgcggtc tgactccttc ttggccgata cttttacgtc catgacgatg ccataggtgc
    12421 ccgagggaac cttgagcgaa gtatctttca catcggcggc cttttcgccg aaaattgccc
    12481 ggagcaggcg ttcctccggg gccaactcgg tttcgctttt gggcgttatc ttcccgacca
    12541 gaatatctcc cggttttacc tctgccccaa ggcgaatcac cccatcaggg ccgagatttc
    12601 gcagggcctc ttcgcccaga ttgggaatgt cgcgcgtgat ttcttccggc cccagcttgg
    12661 tatcacgcgc tcccacctca aactcatcta tatgaatcga agtgaagatg tcttccttga
    12721 cgatgcgctc gctaacgaga atggcatctt caaagttgta tccgttccac ggcatgaagg
    12781 cgaccagcac gttgcgcccc agagccaact cgccaccctg agtgcaggga ccatcggcta
    12841 tgacctgccc tttcttaacg gtctctccct ttttgaccag aatcttctga ttaatacagg
    12901 tggcggcgtt ggaacgcatg aacttgcgga ccgggtaaac gaaaacaccc ttgtccggat
    12961 cgtgcttaat cttcttcttg ccttcgggca gtttgccgtt ttcggtgata ataatctgcc
    13021 cgccggggac ggcggctact ttgcccgccg cttcagccac aataacggcc cgcgaatccc
    13081 gggcgacgcg ttcttcaagc cccgtcgcta ccagcggagc ttccgtgacc agcaggggca
    13141 ccgcttgccg ctgcatgttg gaccccatca gcgcgcgatt cgcgtcgtca tgctccaaaa
    13201 acggaatcaa gctggcggca acggaaacca gttgtttggg cgagacatcc atgtaatcca
    13261 cccgggaagg ttcggcatca ataaagtctc ccttgccccg gcaaacaacc cggtcggtcg
    13321 taaactttcc cttgtcttca atgggcgtat tcgcctgcgc aataacaaag gactcctcgc
    13381 ggtccgccgt aaggtagtcc aattgattgg taacacgtcc cccttcgact tttcgatagg
    13441 gagtctccag aaacccgaag tcgttgatgc gggcaaatgt tgccagggaa gcgatcaaac
    13501 cgatgttcgg cccttccggc gtctccacgg gacatatacg cccgtaatgc gacgggtgca
    13561 cgtcgcgcac ctcaaaccct gcccgatccc gcgaaagccc cccgggtcca agggcggaca
    13621 gccgccgttt gtgcgtcaac tccgccagcg ggttgatttg atccatgaac tgagaaagct
    13681 ggctgcggcc aaagaaatcg cggatgaccg ccgagagtgc tttgggattg atgagcttct
    13741 ggggcgacat ggcttccacc ccctgatcaa acagggtcat ccgctctttg accaggcgct
    13801 ccgtgcgggc caaacccacc cggcactggt tggcgagcaa ctcccctacc gtgcgcaccc
    13861 gccgactgcc caggtgatca atgtcatcca ggctgccctc gcccttcttc aggcgcaaaa
    13921 ggtattttgt cgccgccaca agatccgcct tggtaaggat gcgtgagtcc ccgcctttga
    13981 tgcccagctt ctggttaatc ttgtagcgcc ccacgcggcc caaatcatag cgcttgggat
    14041 cgaagaacaa tcgtttgatc agggcccggg cattcgctgt cgtgggcggg tcgcccggtc
    14101 gcaggcggcg gtaaatgtct ttaagcccct cctcctcgtt cttggcggga tcctttttca
    14161 tgcatttgat gataatcccg tcgtcctgag tcgtatccac gaccctgatt ttgctgaccc
    14221 ccagttcggc tatctgcctg acgaccgctt tggacagcgg ctcaaaagca cgcgcgatca
    14281 ccaatccttc cgcttggtca accgcatctt caatcagcac tttgttggcc aggtcgtcgc
    14341 gcttttcggc ctccttaaca ctgaactcct caatctcgta aaacagcttc aggatctctt
    14401 cgtccgtgcc ccgattcttg tcctggttgt ctttgcccct gccttcgcca tccaggaagg
    14461 ccagggcgcg aaacagggtg gtcgtcagga acttccgccg gcgtttcttg cggtccaggt
    14521 aaacgtaaag caaatcgctg gtgtcaaact gggcttcgta ccatgagccg cggtcaggga
    14581 tgatgcggaa agaatgaagg gtttttccat tgggatgcag cgtcgcctca aaggccaggc
    14641 cgggggaccg gtgcaactgg ctgacaataa cgcgctcggc gccattgatt acaaaggtgc
    14701 cctgcggagt catcaggggc aattccccca tgaacacctt ctcctctttg acggttcttt
    14761 cctccttgag ctggaaagtg acgtacaggg gcgcgccata ggtcaagccc tcccgcaaac
    14821 attccagcca gtccactttg ggttctccta tttcatagga atgaaaatcc agaacgcact
    14881 ttccatcgta gctttcgatg ggaaacactt ccgtgaaaac ggcctgcagc cccaggtttt
    14941 cccgcttcga gggagcgaca ccggcctgga gaaaatcccg gtacgagttg gtctgcaatt
    15001 cgatcagatt gggcggggcg attatttctt tgatcttgcc ggaattgatt cgttgacttg
    15061 ctcgcgttga cattgggccc tttaataaat ggcgggcggc agcgccacgc cgttggttac
    15121 agctaaacaa cacaaggcaa gaacgcagcg ttcacgcgcg aacttgccga aactcatacc
    15181 gcctgaggcg gcctggctga caatctgtcg gctaatctaa aactttcgtt attcggtcaa
    15241 aaccaaccgg agggggcggc ggcgtcaaac cgccgccccg acaaggaacg accggtcact
    15301 tcaattcaac cttggcgccg gcgtcttcca gcttcttctt ggcggcatcg gcctcagcct
    15361 tgctggcacc ctccagcacc gtcttgggcg cgccctcgac cagggccttg gcttccgcca
    15421 gcccaagacc ggctttaacc tcgcgcaccg ccttgatcac cgaaatcttc ttgtccgccg
    15481 ccactgcagt caaaataaca tcaaactgcg tctgggcttc cgcagctggc gctgccgccc
    15541 cggctcccgg agccgcggct gcgaccgcca ccggcgcggc ggccgaaacg ccccacttgg
    15601 tttcaagttc cttcaccaga tcggcgatct cgatcaccgt cgcattactc aactcttcta
    15661 cgatttttgc tttatctaga gccatggtta cctttcatct cgtctcgtcg tcccccgcct
    15721 ttccgggtga attaagcgca ttgtctatcc gccgacgatt tacgttttgt ttgtttgttc
    15781 ccactccccg acggcttaaa tcggggaagt gaaatctgtt ttaccgtcaa ttcgccgccg
    15841 gcttggcggc ctccgcctgc tgatccgccc gggcttgcag aatccgcgcc atctgctcgg
    15901 cgggagtgtt caacagcctg accagggtgg tggccggggc gttaaacaaa gcaagcaatt
    15961 tggcccgcag cacctccagc gagggcaaat ccgccagcat catgatctcc ggctgctcaa
    16021 gccgctggtt gttcaaatag ccaaattgca ccttcaggcg gtccagttcc gtcccaaaag
    16081 ttttaacaac cttggccgcg gtggaaatat cgcgctgccc ggttaccaca gccagttgcc
    16141 cgctcaggct accgtttaaa tcgcccacac cggcttcccg ggcggcctca cggaacacgg
    16201 agttcttcac cacatgaatt tccgcgccag ccgcactcag ccgtttgcgc agttcgatca
    16261 actggacaac cttaaggccc ttataattga ccacgataaa gaacggcgaa gcgttcaacc
    16321 gcgccaggta ttctttcgtc aggttttgct tttcgggacg catatgcttt taaacttgtt
    16381 aagccccctg ggcgataata ccactcacac cgcgaattcc ttcaggtcca gccgcacggg
    16441 cggactcatg gttcccgaga gcgtgcaggt tttaatatac gtgcctttga cgctctgcgg
    16501 ccgggctttg gccaccgcat caatgaccgc gcgcacattc tcttcaatct gggtcgccgc
    16561 aaacgccaac ttcccaaccg gcacatggat attgcccgcc ttgtcaactt tgaactccac
    16621 gcgccccgcc ttgacctcct tgaccgcgcg cgccgtgtcc tccgtcaccg tccccgtctt
    16681 ggggttgggc atgaggccgc gggggcccag caccttgccc agcttgcgca cttcgcccat
    16741 cgcttccggc gtggccacgg ctacgtcaaa atccgtccac ccctcctggc acttcttgag
    16801 catatcctca aagcccacat attcagcgcc agcacttttg gccgcctcag ccgccggccc
    16861 gctcttggca aaaaccagca cgcggacggt cttgccgctg ccgtgcggca acggcaccgt
    16921 gccacggacc atctggtcgg attgcttggg gtcaaccccc aggcaaaacg ccagttccac
    16981 ggtttcatta aacttggctt tgggaaactt agccagaacc tcaaccgcat ctttcagcgg
    17041 gtaagtcttc cggacatcca ctaattcgag tgctttgcga taacgtttac ttggcattta
    17101 gtctggtgcg gtgcaaacga gcctgcgctc tcccgcggtt attcaaatga acaaatttct
    17161 actttactca accgacaacc tcaatcccca tgctgcgcgc cgtcccggcc acaacacgaa
    17221 acgccgcttc ttcagaggtg gcattcatat ccttcagctt gatcttcacg atatccagga
    17281 cctgcttgcg cgtcactttg ccgacctttt ccttgttggg ttccttggaa ccagcggtaa
    17341 tccccgccgc tttcttgagc agaaccgctg ccggcggcga cttggtgata aacgtaaagg
    17401 acttatcctg ataaaccgta atgaccaccg gaataatcgt accggtctgg tcccgggtct
    17461 tggcattata ctctttgcag aaggccatga tattgacccc gtgctgtccc agggccggcc
    17521 cgaccggcgg cgcgggattg gcctgcccgg ccgggatctg caacttaatg actccagtaa
    17581 tcttctttgc cataatcttt atttggtctg gcggacatcg cggagcgcgc ccgccaagcg
    17641 gtcaacccgc ccggccgcac tccgttctgg tatcaccctt tttccacctg ccagtactcc
    17701 agttcgacgg gcgtgttccg cccaaaaatg ttaaccgtca ctttcaactt gccccgttcc
    17761 ggctcgattt cctcgatcaa gccgctgaaa ttcaggaagg gcccattatt aatcttcacc
    17821 gtctccccta cttcaaagct gaccttcggc ttctcatact cctccgaagc ggagatctgc
    17881 tttttaatgg cctcgacttc ctccgccggg gtcggggcgg gcggttcgcc gcctacaaag
    17941 ccgataactc ccggcgtatc ctggataaaa taccacggct tctcgacaat ttgcttgttt
    18001 tcatcgagca gcaccatgtc cacaaacaca taccccggcc agagtttgcg ggaggaaacc
    18061 gtcttcttct ggttccgcac ttccaccact ttctccatgg gcaccagcac ctcttttatg
    18121 taatcaccca tctcctccgc cttgaggcgc ttctcgatgc tctccttgac cttctgctcc
    18181 tggccggcga gcgtatggat gataaaccac tgacttcgca tggcctcaga gcttgaagaa
    18241 catgaacagc cggaaaaata cctggtccac cagcacgatg aacgtgccca gcaacacaat
    18301 cgaaatggtg atgaccacgg ttgacccctt aagctccccc cacgtcggcc aggtgcactt
    18361 gcgcaattcc tcgcgcgtct gctgtacata gttggtcaac tgcaataagt accccttccg
    18421 ccagcaatac gcaaaggcgg ctccaatgat cacagcccat atcagcagcg ttatcataca
    18481 acgttattca aaatcttatc atgtcctggc ggagagggag ggattcgaac ccccgtcggc
    18541 ggttaagccg aagcggtttt caagaccgcc gcgatagacc actctgccac ctctcc
//
LOCUS       DAOPQG010000074        18100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3713_length_18100_cov_16.224993, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000074 DAOPQG010000000
VERSION     DAOPQG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 18100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18100
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3713_length_18100_cov_16.224993"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKI07_09410"
     CDS             <1..630
                     /locus_tag="PKI07_09410"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC51308.1"
                     /translation="WRHNGVPLAGATASSYTRNNVEISDAGNYSVVITNVAGSVTSLD
                     AVLVVNVPPTITTQPQSQAVIQGGNASFTVAAAGTAPLVYQWYFDGQPLADATDSALT
                     ITNAQPADAGSYAVEVLNLAGQIMSDPATLTVNAPPQIDEIRVLADGRFELQISGAPG
                     QYVVEATPDVAGVVSWTALTNFTVTTTNFQYYDSETGLARRFYRVRWTP"
     gene            960..7067
                     /locus_tag="PKI07_09415"
     CDS             960..7067
                     /locus_tag="PKI07_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HOC51309.1"
                     /translation="MSNEKPGSEVHVMTQPHAILSRRPRRSGAYRRSSWLLAALLIGL
                     GSPLSLRAAEEFRAAWADVFHVGTGSAAEVDTMVSTLVSGRYNVVIVQVLGYMDRNGT
                     ASHGAHWKSSILPWSPRVTASFDPLAYLCTKAHAQGIQVHAWLGGSGGGPYRVSTVWP
                     PPNNATLAAHPEWFIAPYANSEGGSPKLVDGNYSLDMGSPDVQEYLVSIVRELVTNYP
                     IDGINWDDELNSTGYNEGFGYPAYSQANYARSGLARFRINTGYVGTPANTHTAWSNYR
                     RRFKNELIARVQAEIQSIKTNPRQPLRHTIAPIAYSPVPGSCTFSGSAPYTYFCDWAG
                     MLQNGYVDAAIPQTYSSSTFNTWVDRCVGCWQYNRQIFSGIGAYLYSNATIADEISYL
                     RSKGLKGYSTYSYAVPTSGSGWWSYAAANVNTSTATVPTMPWRNPATATEGIVWGRVK
                     DASTGLYVDDATVTVAGGPTVKTDGNGYYVATLVPATAGGTAHATTASKTGMTPQTVT
                     AIALAGDVVRYDLNLNAATNVAPTIITQPQSLAVNQGGSATFTVAATGTAPLAYQWRR
                     NGAGIAGATLSSYTKNNVQTSDAGSYSVVVTNLAGSATSSNATLTVIVPPTIITQPLS
                     QTVTETANVTFTVAASGTAPFAYQWRFNGVNVAGATLSSYTKNNVQLADAGSYSAVVT
                     NLAGSATSANAILTVQALPTPPAIITPPISQTANQGGSATFTVTASGSAPLAYQWRWF
                     GTNLAGATGTSLTLANLATNQSGPYTVVVTNAYGAITSAVATLTVTPTLEVGGLAVLW
                     NLAPGSRPYLTTTVGGPPDERGMAYNPLTRRVIVVQRSTTTAYVLDGDTGADLWTLNT
                     TGVTGGYTSTYYLLMVGVAEDGVVYAGNMTLHGNTTSFKLYRWANDSAGTVPTVAYSG
                     DPGAGQDLRWGDTLDVRGAGASTQIIIGSLNTSHFAVLTTANGTNFTSKLVTLTDAPG
                     GAVGSGLAFGTGNTFWCKAGLPAPLNLRQASFDLAAGTATTVRNYADPAFPDSLGPIG
                     VNPSLDLLGGVYAAAANGGNSFRLYHLVPTNSAPGFITSTNFATDNDNSYSGTGAVDF
                     GGDRVYALSSNNGLLAMQLVPPAALIPPSIVTHPVSQTVNQGGSATFTVSATGSAPLA
                     YQWRFNGTNIAGATGSSYTRANVQPADAGNYSVFVSNDAGNATSANAVLTVTVPASPP
                     VISAGPQSQTVIAGQNATFTVTATGTAPLNYQWRFNGANLAGATASSYTRANVQVADA
                     GAYTVVVSNSYGSVTGAVATLTVHFTLTTSVAAGGTVSKTPDQSSYAPGAPVTLTATP
                     SAGYEFSGWSGDASGTNNPLTVVMGGNLVIAANFTVVCDLILDNTDPEVSYVGTWQTG
                     TAAGKYGADYRFALGSAVGNSNVTYRPNICADGRYDVYLWYLQGANRATNAPWVISYS
                     GGTTNIRVNQQINGSRWWRLASALPFTQGTAGFARLYNTNATGGTSGSTVVLADAVRF
                     AYVGPLTTPTATTLSSSANPSVYGDAVTLTATVTGSGGTPSGLVTFKDGATVLGTAIL
                     NGSGQAVWTTTTLSASGSPHSLAAVYSGDSSFAASTSVALSQVVNPKALTVTGATVAS
                     KVYDGTTAATISGAALNGVVSGDAVALGNAASGTFANKHVGVGKAVSTAMTLSGADAG
                     NYTLTQPALTGTITARALTVMGATVASKVYDGTTAATISGAVLNGVISGDAVALGNAA
                     SGTFANKHVGVGKAVSTAMTLSGADAGNYTLAQPALTGTITARALTVTGLAAQNKVYD
                     GTTTATLAGTPGLAGAVSGDGVALAGTATGTFANADVGTAKTVTISGLSLAGADAGNY
                     TLTPPTATADITGAATTTTLISSVNPVGVGSNVTFTATVVSGAGTPAGTVVFLAGGVP
                     FHTNTLAGGVAAASTDALSPGTNTIRAEYAGGGNYLGSDGSLEQVVKAFLVCGQTNVL
                     LGILNNQDGTFTLLLAGTPQADYYIVSSSDPLLPAANWMPLPGSTNTVTNAGGFWQFT
                     VTNAFTQQFYRSVSVVPCP"
     gene            7270..8610
                     /gene="tig"
                     /locus_tag="PKI07_09420"
     CDS             7270..8610
                     /gene="tig"
                     /locus_tag="PKI07_09420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOC51310.1"
                     /translation="MTVQSLTPCKKLVRVEVEAAKVDETFEAVFKDFRREASLPGFRP
                     GKVPREMLLRIYEKDIAEETKRKLIKETYRKAIEEQKLDVVTPPDIEEIQFSRGQPLV
                     YNATLETAPEFELPEYKGLPVKREVRAVTEADVEKALNLLREQRATYEKRERPLQTGD
                     IAVVNYQGTCDGRPITEIAPTAKGLTEQKDFWVQTQPDSFIPGFAAQLLGAKAGDRRT
                     VTVDFPADFVTSQLAGRKGVYEVEVVEVKERVIPPLDDELAKGYGAENLAKLTDGVRE
                     DLENELKYKQKQALRDELLRTLLGRVTFDLPETAVTRETSNVVYDLVQENAKRGVSRQ
                     VIEQQKDQIYTIASQNAKERVKLQFLLQKIAEKEDIKVSTEEINQRLGYLAQLYQIPV
                     GKVAKDLQKRDGLIEIYDQIMNQKVIKLLEDNARIEDVQPVTPLPAEAPAAPPG"
     gene            complement(8693..10660)
                     /gene="mutL"
                     /locus_tag="PKI07_09425"
     CDS             complement(8693..10660)
                     /gene="mutL"
                     /locus_tag="PKI07_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOC51311.1"
                     /translation="MNRIRLLPEQVANQIAAGEVIERPASVVKELVENALDAQARRVV
                     VEIQAGGRSLVRVGDDGLGMARDDALLCLERHATSKIRRAEDLAALATLGFRGEALPS
                     IASVSRFTLTTRAREGDSPEGTQIIVHGGRILEVKAAGRAPGTTVEVRQLFFNLPARR
                     KFLRTEETESAHIQHYLTLAALAHPEVAFAFQRDGRPVWQLPPVKAGDGSAARLGALQ
                     ERLRILYGGERALLRVDYSAALEPPDDPDAETAPAPARPPAPAAPFRLWGFIGAPGVS
                     RSNREDQHLFVNRRPIENRGLNFALLEGYHTALMKGRYPVACLFLEIDPAMVDVNIHP
                     AKREVKFHREAEVRRLVAQAIRQTLLAFHSGTPASAAPPAAAPGPPEPAAPPPLAPPP
                     PPAAQSALPNFPAALAPAPQPRPLTASATPPLRMGFSPAPAPPATPAVLPPTAPGPLP
                     PPPLPEPVPLLKVPLRLVGVIGRLYVVLESDRGLVLLDQHAAHERILFEQMLRRLEQH
                     DQAPSQRLLLPETVELAAREAGFLREQLSVLTRLGVGLSEFGERTFLLDALPPFVKVP
                     DARRFVIELVDELRAAGRELNALRLGEDTIAKTVCRHAVKANDPLAGRELENLVEDLR
                     HCAMPYTCPHGRPTLIEMSFRELEKKFGRTQ"
     gene            complement(10657..11808)
                     /locus_tag="PKI07_09430"
     CDS             complement(10657..11808)
                     /locus_tag="PKI07_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOC51312.1"
                     /translation="MVALETSKLFSPLSPSELSALSRQARERSFAAGEEIFREGDRGD
                     GLYVVRSGRVEISGSVDSGARLVFSEVGPGEVFGEMAVVENQPRSAWAVAREDTRVYF
                     IPRDEMLALVARSPALALALLQEISRRLREFNQQYLRQVLQAERLAIVGRFARSIVHD
                     LKNPLNIIGLTAEVAGQKRASLKARQQAAGHIRKQVERISDLINEILDFTQGAPANFV
                     LAPVNYGAFVAQLLEELGPEAGLKSVALALANPPPSVEVFIHPKRLRRVFYNLVHNAT
                     DAMPSGGRVVFRFQVTAKEVVTELEDGGPGIAPEMAGQLFQVFATHGKAHGTGLGLFI
                     CKRILTDHGGWIAARNLPDGGAVFSFGLPRRPPEAAAAASLSGPAPATP"
     gene            complement(11947..12162)
                     /locus_tag="PKI07_09435"
     CDS             complement(11947..12162)
                     /locus_tag="PKI07_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51313.1"
                     /translation="MRNTATGLYFQGPGMWTSDPAVARDFKLIDRALQFIETWRLKDM
                     ELAFAFRNVNRVTRVPIEKTTLNYLES"
     gene            complement(12390..12956)
                     /gene="pyrE"
                     /locus_tag="PKI07_09440"
     CDS             complement(12390..12956)
                     /gene="pyrE"
                     /locus_tag="PKI07_09440"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOC51314.1"
                     /translation="MTESEVLQIFRASGALLEGHFVLRSGRHSRQYFQCALALQQMPI
                     VEKLGGELARRVGALGAATVVAPAMGGLVLGQEVARQLGRRFIFVEKEQGKLALRRGF
                     RIAPGEKILIVEDVITKGGRVQETLDIVRAHQGRVVGVVVAVDRSGGEVNFGVPLFSL
                     VTLQVETFAPDQLPDDLAALPAVKPGSA"
     gene            13038..13691
                     /locus_tag="PKI07_09445"
     CDS             13038..13691
                     /locus_tag="PKI07_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51315.1"
                     /translation="MPKWCKTILAILLLPACAGAVAALGQLLRAAGGADRTWVPFLAG
                     AACWITVFLLLPKPMRLYVFGHELTHALWTWLFGGRVKRFRATARGGHVVVNKNNFVI
                     ALAPYFFPLYAVLVVLGFLLGHALWDWRHYLAGFHLLLGAAYAFHLTLNWHILQFDQS
                     DIRDQGYLFSAVIIFLGNVAVLLVGIPLLVPRVGVLTALGWWLRGTGEVLARIGRIL"
     gene            13818..14282
                     /locus_tag="PKI07_09450"
     CDS             13818..14282
                     /locus_tag="PKI07_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOC51316.1"
                     /translation="MDLADIITKEQIITDLRATNRWEAIDELTDNLVATGKISPPHRE
                     AVIAAVKKREASMSTGIGFGVGIPHASTDLITSVVAALGRSRKGVDFDSLDNQPVSLV
                     LLFLVPQGQFQKHLHTLANIAKLLHNADFRQTLEQAPDSAAMFQAIRSHGLK"
     gene            14376..16097
                     /gene="argS"
                     /locus_tag="PKI07_09455"
     CDS             14376..16097
                     /gene="argS"
                     /locus_tag="PKI07_09455"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOC51317.1"
                     /translation="MLPAQLIEQRLQAAVKTVLPDADTAAVLVRPCPDPRFGDYQTNA
                     LMGLARARKLNPRQLAADVLARLDVADCCEPPEVAGAGFLNFRVQTAALARALQAAAR
                     GEHLFFPPAAAPRTVVVDFSSPNVAKSMHVGHIRSTILGDSLARVLRLLGHRVITDNH
                     LGDWGTQFGMLLTAWKRDLNAAALQTDAIAEMERLYKQAAAECKTSPAALEAARRELV
                     RLQAGDAENLAIWRRMIALSRAQFDIIYQRLGVRFDHTLGESFYHPRLQPLVQELCDR
                     GLARESEGARVVFFDDLPALQAHPALIQKRDGAFNYTTTDLATLAYRLETWQPDEIIY
                     VTDARQQLHFQQLFAAFRRWRPEAAVRLAHVWFGSILGEDNKPFKTRSGETVKLADLL
                     DEAEERAYRAVSEKNPALAEADRREVARVVGLGALKYADLLPNRQSDYVFSWDKMLAL
                     NGNTAPYLQYACARIRSIFRKGDVGGDLAPAALELGAPEELALARHLLNFGLVLAAVA
                     EEYRPNFLCNYLYELAGRFTAFYEHCPVLKSAPGPRASRLVLCDLTARVLQQGLAVLG
                     IETLEQM"
     gene            complement(16103..17542)
                     /gene="uxaC"
                     /locus_tag="PKI07_09460"
     CDS             complement(16103..17542)
                     /gene="uxaC"
                     /locus_tag="PKI07_09460"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOC51318.1"
                     /translation="MSFIHPDFLLQTRTAARLYHQYAADEPIFDYHCHLSPGDIARNR
                     QFANLFEIWLEGDHYKWRAMRANGVPERFCTGDASPFEKFKAWAATVPRTLRNPLYHW
                     THLELKRYFGIDEPLEERSAARIWKQAAERLATPGLSAWGILRRFKVAVVGTTDDPTD
                     DLAHHRALAASGLATRVLPTFRPDRGLAVHQPVPFNQWVQRLEAAANVEIRGLGSFLE
                     ALEKRHDYFHACGCRLSDHGLARCYTEDTTLKTAGAIFTRARRGRAASPAEQARFGAL
                     LMRFFGRLNAGKDWTMQLHLGPLRNTNTRRLGQLGPDTGFDSIGDYPQGVALAAFLDA
                     LDREQALPRTILYNSNPADNYLFAAMTGNFPGDGRAAKVQMGSGWWFLDQKEGMEWQL
                     NALSNLGLLSRFVGMVTDSRSFMSFPRHEYFRRVLCNLVGRDAENGELPKDLKRLGPM
                     VRDICYANAQQYLALPGVGRAGGGKSRPR"
BASE COUNT         3253 a         6225 c         5656 g         2966 t
ORIGIN      
        1 tggcggcata atggggttcc gctggcgggc gccacggcga gcagctacac ccggaacaat
       61 gtagagatca gtgacgcggg caattattcg gtggtcatta ccaatgtggc cgggagcgtg
      121 accagtttgg atgcggtttt ggtggtcaat gtgccgccga ccatcaccac gcagccgcag
      181 agccaggccg tgattcaggg cgggaatgct tcctttaccg ttgcggccgc cggcacggcg
      241 cccctggttt accagtggta ttttgatggc cagccgctgg cggatgccac cgacagcgcg
      301 cttacgatta ccaatgccca gccggccgat gcgggcagct acgcggtgga agtgcttaat
      361 ttggccgggc aaatcatgag cgatcccgcg acccttaccg tcaacgcgcc gccgcagatt
      421 gacgaaatcc gcgtgctggc cgacggccgg tttgagttgc agattagcgg ggcgccgggg
      481 cagtacgtag tggaggccac gcccgacgtg gcgggagtgg taagctggac ggcgttgacc
      541 aatttcaccg tcacgaccac caacttccaa tactacgaca gcgagaccgg tttggcccgg
      601 cgcttctacc gggtgcgctg gacgccataa gcggaacgac gccgttgcgg gcgtgaagca
      661 cgcgcttctc gcgcgtcgcc gccgattcct atggggaatt ggcgcgcggg gcgcgggatg
      721 tgccatttcc cgccggcgca tttacggtta tcgcgccggc ctgcaagggg tgttaaaatt
      781 cgctaaatcc gctaaatagt gcttgctttt tgcgggaaaa gatgcgaatc tggctggcga
      841 atgtgccgtt tagatgccgc gatcgtcgta tgctctctct aggcgaggaa aggtgtgcct
      901 cgtaattccc tatgatttga gtgaacccgc gacttctggc aacaactcca gacaaaacta
      961 tgtcaaacga aaaaccgggc agcgaagttc atgttatgac ccaacctcac gcaattcttt
     1021 cccgtcggcc gcgccgttcc ggggcctacc ggcggagttc ctggctgctg gcggccctgc
     1081 tcatcgggct tggcagcccg ctgtcgctgc gggcggcgga ggagtttcgg gcggcctggg
     1141 cggatgtgtt tcacgtcggc acgggcagtg ccgccgaggt ggatacgatg gtctcgacgc
     1201 tcgtctccgg ccgctacaat gtggtcatcg tccaggtgct gggatacatg gaccgcaacg
     1261 gcaccgcctc gcacggcgcc cattggaagt catccattct gccgtggtct ccgcgcgtga
     1321 ctgccagctt tgacccgctg gcctatcttt gcacgaaagc gcacgcccaa ggcatccagg
     1381 ttcatgcctg gctgggcggc agcggcggcg ggccttaccg cgtctcgacc gtctggccgc
     1441 cccccaacaa cgccaccctg gcggcgcatc cggagtggtt tatcgcgccg tatgccaata
     1501 gcgagggagg gtctcccaag ctggtggatg gcaattactc gctggatatg ggttcgcctg
     1561 acgtgcagga atacctggtc agcatcgtgc gcgagttggt gaccaactat ccgattgacg
     1621 gcatcaactg ggacgacgag ctcaacagca ccggctataa tgagggcttc ggttatcccg
     1681 cttacagcca ggccaactat gcgcgttccg ggctggcccg gttccgtatc aacacgggct
     1741 acgtgggcac gccggccaac acccataccg cctggtccaa ttaccggcgg cgctttaaga
     1801 acgaattgat cgcccgggtt caggcggaga ttcaatccat caagaccaat ccgcgccagc
     1861 cgctgcggca taccatcgcg cccattgcct acagcccggt ccccggttcc tgcaccttct
     1921 ccgggtctgc cccctacacc tatttctgcg attgggccgg catgttgcaa aacggctacg
     1981 tggatgccgc gattccccaa acctacagca gcagcacttt taatacgtgg gtggaccgtt
     2041 gcgtgggttg ctggcaatac aaccggcaga tcttctcggg cattggcgcc tatctttaca
     2101 gcaacgccac catcgccgat gaaatcagct atcttcgcag caagggcctg aaaggctaca
     2161 gcacctactc ttatgcggtc ccgacctcgg gcagtggctg gtggtcctat gccgccgcca
     2221 atgtcaacac gagcaccgcc accgttccca ccatgccgtg gcgcaacccg gcaacggcga
     2281 ccgagggtat tgtgtggggc cgggtcaagg acgccagtac cgggctttat gtggatgacg
     2341 cgaccgtgac cgtggcgggc gggccgacgg tcaagacgga cggcaacggt tattatgttg
     2401 ccacgctggt tccggcgacg gcgggcggca ccgctcatgc caccacggcc agcaaaaccg
     2461 gcatgactcc gcaaaccgtg actgcgatcg cgctcgcggg ggatgtcgtc cgctatgacc
     2521 ttaacctcaa tgccgcgacg aatgtggcgc cgacgatcat cacccagccg caaagcctgg
     2581 cggtcaacca gggcggcagc gccaccttta cggtggccgc caccggcacc gcgccgctcg
     2641 cctaccagtg gcggcgtaat ggggccggca ttgccggggc gaccctcagc agctatacga
     2701 agaacaacgt gcaaacgagc gatgccggca gctactcggt ggtggtcacc aacctcgccg
     2761 gcagcgccac cagttcgaac gctacgctga ccgtaattgt gccgccaacg atcatcaccc
     2821 agccgttgag ccagacggtg acggagaccg cgaacgtcac gttcacggtg gccgcctccg
     2881 ggacggcgcc attcgcctac caatggcggt ttaacggggt gaacgtggcc ggggcgaccc
     2941 tgagcagcta taccaaaaat aatgtgcaac tcgcggatgc cggcagctat tccgcggtgg
     3001 tcaccaacct cgccggcagc gccaccagcg ctaatgccat tctgacggtg caggccctgc
     3061 cgactccgcc ggccattatc actccgccga tcagccagac ggccaaccag ggcggcagcg
     3121 ctacgttcac cgtcacggcc agcggcagcg cgccccttgc ctatcagtgg cgctggtttg
     3181 gcacgaatct cgccggggcc accggaacca gcctgaccct ggccaacctc gccacgaacc
     3241 aaagcggccc ctacacggtg gtggtcacca atgcttacgg cgccatcacc agcgcggtgg
     3301 cgacgctcac cgtcaccccg acgctggagg tgggcggatt ggcggtgctg tggaatttgg
     3361 cgcccggctc acgcccctac ctcaccacga ccgtcggcgg cccgcccgac gagcgcggca
     3421 tggcctacaa cccgctgacc cggcgcgtga ttgtcgtgca acgcagcacc acgacggcct
     3481 atgtgctcga tggcgacacc ggggccgacc tgtggacgct gaacacgacc ggggttacgg
     3541 ggggatacac gtccacttat tatttgctga tggtgggagt ggcggaggat ggcgtggttt
     3601 atgccggcaa tatgacgctg catggcaaca ccaccagttt caaattgtat cgctgggcca
     3661 atgacagcgc cggcacggtg cccaccgtcg cctattcggg cgacccgggc gcggggcagg
     3721 atctgcgctg gggagacacg ctggacgtgc gcggcgcggg cgccagcacg cagatcatca
     3781 tcggttcgct gaacaccagc cacttcgccg tgctcaccac ggcaaatgga accaacttca
     3841 cctccaagct ggtaaccctg accgatgcgc cgggcggcgc cgttgggtcg gggctcgctt
     3901 tcggcaccgg aaacaccttc tggtgcaagg ccggtttgcc ggctcctctg aacctgcggc
     3961 aggcttcctt tgacctggct gccggcacgg cgaccacagt gcgcaattat gccgacccgg
     4021 ctttcccgga cagccttggc cccatcggcg tgaaccccag cctcgacttg ctcgggggcg
     4081 tttatgccgc cgctgccaac ggtggcaaca gcttccggct ctaccacctc gtgccgacca
     4141 acagcgcgcc cgggttcatc acgtccacca actttgccac ggacaatgac aacagctata
     4201 gcggcacggg agcggtggac tttggcggcg accgggtcta tgcgctcagc tcgaacaacg
     4261 gcctgctggc catgcagctt gttcctcccg ccgccctcat cccgccgtcc attgtgactc
     4321 acccggtcag ccagacggtc aaccagggcg gcagcgccac ctttaccgtc agcgcgaccg
     4381 gttccgcgcc gctcgcctac cagtggcgct ttaacggcac caatatcgcc ggtgccaccg
     4441 gcagcagtta tacccgcgcc aatgtccagc ccgccgatgc cggcaactac tcggtcttcg
     4501 tttccaatga cgccggcaac gctaccagcg ccaacgccgt cctgaccgtg accgttccgg
     4561 cctcgccgcc ggtcatcagc gccgggccgc aaagccagac cgtcatcgcg ggccagaacg
     4621 ccacgttcac cgtcaccgcc accggcaccg cgcccctgaa ttaccagtgg cggtttaacg
     4681 gggcgaatct cgccggggcc accgccagtt cctacacacg ggccaacgtg caggtcgccg
     4741 atgcgggcgc ttatacggtg gtggtaagca acagctacgg atcggtcacc ggcgcggtgg
     4801 cgacgctgac cgtccatttc accctgacga cctccgtcgc ggccggcggc acggtatcca
     4861 aaacccccga tcaatcaagc tatgcgccgg gcgcgcccgt gaccctcacc gcgacgccgt
     4921 cggcgggcta cgagtttagc ggatggtcgg gggacgcaag cggaaccaac aacccgctca
     4981 cggtggtgat gggcggcaac ctggtgatag ccgcgaattt caccgtcgtc tgcgatctca
     5041 tcctggacaa caccgatccg gaggtcagct atgtgggaac gtggcaaacc ggcaccgcgg
     5101 cgggcaaata cggggctgac taccgcttcg cgctcggttc cgcagtcggc aattcaaacg
     5161 tcacctaccg gcccaacatc tgcgccgacg ggcgctatga tgtctatctc tggtatttgc
     5221 agggcgccaa ccgcgccacc aacgcgccct gggtgatttc gtattccggc ggcaccacga
     5281 atatccgggt taaccagcaa ataaacggca gccgatggtg gcggctggcc agcgctctcc
     5341 cctttacgca gggaaccgcc ggcttcgccc gcctctataa cacgaatgcc acaggcggta
     5401 catccggcag cacggtcgtg ttggcggacg ccgtgcgttt cgcgtatgtc ggaccgctga
     5461 cgactcccac cgccaccacg ctcagttcct cggctaatcc ttccgtttat ggcgatgccg
     5521 tcacgctgac ggctaccgtc accgggagcg gaggcacgcc gagcggcctc gtcactttca
     5581 aggacggcgc gacggtcctg ggcacagcca tcctgaatgg ctccggccag gcggtctgga
     5641 ccaccaccac cctttcggcc agcgggtctc ctcattccct ggcggcggtc tatagcggcg
     5701 acagctcttt tgccgccagc acttcggtcg ctttatcgca agtcgtgaac ccaaaagcct
     5761 tgacggtgac gggcgcaacg gttgccagca aggtatatga tggaacgacc gccgcgacga
     5821 tttccggagc ggccttgaat ggcgtggtca gcggcgacgc cgttgcgctg ggcaacgccg
     5881 ccagcggcac gttcgcaaac aagcacgtcg gcgttggcaa ggcggtcagc acggccatga
     5941 cgctcagcgg cgccgatgcc ggcaactaca cgctgacgca gccggcgctt acgggcacga
     6001 tcaccgcccg ggcgctgacg gtgatgggcg cgacagtcgc cagcaaggta tatgatggaa
     6061 cgaccgccgc gacgatttcc ggcgcggtct tgaatggcgt gatcagcggc gatgccgttg
     6121 cgctgggcaa cgccgccagc ggcacgttcg cgaacaagca cgttggcgtt ggcaaggcag
     6181 tcagcacggc catgacgctc agcggtgccg atgccggcaa ctacacgctg gcgcagccgg
     6241 cgctcacggg cacgatcacc gcccgggcgc tgacagtgac gggcctggcg gctcagaaca
     6301 aggtatatga tggcaccacc acggcgacgc tggcagggac tcccgggctt gcgggcgcgg
     6361 tgagcggcga tggcgttgcc ctcgccggca cggcgacggg aaccttcgcc aatgcggacg
     6421 tgggcacggc caagaccgtg actatttccg gcttgagcct ggccggcgcc gatgccggca
     6481 actacaccct gaccccgccc accgcaacgg cggacattac cggggcggcc acgacgacca
     6541 cgctgatttc ctcggtcaac cccgtgggcg tgggttcgaa cgtgaccttt accgcgaccg
     6601 tcgtttccgg agcgggcacc ccggccggca cggtggtctt cctggccggc ggcgtgcctt
     6661 tccacaccaa tacgctggcg ggcggcgtgg cggcggccag cacggacgcg ctgtcgccgg
     6721 gcaccaacac catccgtgct gaatatgcgg gcggcggcaa ctacctgggc agcgacggca
     6781 gcctggagca ggtggtcaag gccttcctgg tttgcggcca aaccaacgtg ctgctgggca
     6841 ttctcaacaa ccaggacggc accttcacgc tgctgttggc gggcacgccg caggcggact
     6901 actacatcgt gagcagttcg gatcccctgc tgcccgcggc caactggatg ccgctgccag
     6961 gcagcaccaa caccgtcacc aacgccggag gcttctggca attcacggtg accaatgcgt
     7021 tcacgcagca gttctaccgc agcgtgagcg tagtcccgtg cccgtaaaag cggccggaaa
     7081 cggattcccg ccacggaacc tcgccgcgcc gcgcaccccg cggcggcgcg gcccgcgtgg
     7141 ggcggccacg tccggcttga cccccggcgg gcgctggcct agtgtaatcc tttcgctgag
     7201 cgccggacac tcaaccaatc ctgcttgcag ggttttctcg gcgattatgg aaagggtttg
     7261 ctagtgaacg tgacagtgca aagtttgacc ccgtgcaaga agctggtgcg ggtggaagtg
     7321 gaagcggcga aggtggatga aaccttcgag gccgtcttca aggattttcg gcgcgaggcc
     7381 agcctgcccg ggtttcggcc cggcaaggtg ccgcgcgaaa tgctgctgcg gatctacgaa
     7441 aaggacattg cggaggaaac gaagcgaaag ctcatcaagg agacctatcg gaaggccatc
     7501 gaagagcaga aactggatgt ggtgacgcct ccggacatcg aggagattca attcagccgc
     7561 ggccagccgc tggtgtataa cgccaccctc gagaccgcgc cggagttcga gttgcccgaa
     7621 tacaaagggc tgccggtcaa acgcgaagtg cgtgccgtca ccgaggccga tgtcgagaaa
     7681 gcgctcaacc tgttgcggga gcagcgggcc acttacgaaa agcgcgaacg accgctccag
     7741 accggggaca tcgccgtggt gaactatcag ggcacctgcg acggccggcc gattaccgaa
     7801 atcgcaccga ccgccaaagg cttgaccgag caaaaggatt tctgggtgca aacccaaccc
     7861 gactctttca tccccggttt tgccgcccaa ctcctgggcg ccaaagccgg cgaccgccgc
     7921 acggttaccg tggacttccc ggcggatttt gtgacgtcgc aactcgccgg ccggaagggc
     7981 gtttacgagg tggaagtcgt cgaggtcaaa gagcgggtga tcccgccgct ggatgacgaa
     8041 ctggccaaag gctacggggc cgagaacctg gcgaaactca ccgacggagt gcgcgaagac
     8101 ctggaaaacg agctgaagta taagcagaag caggcgttgc gggacgaact gctgcgcacc
     8161 ctgttgggcc gcgtcacgtt tgatttgccc gaaacggcgg tcacccggga aacgagcaac
     8221 gtggtttacg atttggtgca ggaaaacgcc aaacgcggcg tttcccgcca ggtcatcgag
     8281 cagcaaaaag accagatcta caccatcgcc tcccaaaacg ccaaagagcg ggtcaaactc
     8341 cagttcctgt tgcagaaaat agcggaaaag gaagacatca aggtctcgac cgaggaaatt
     8401 aatcaacgcc tgggatacct ggcccagctt taccagattc cggtcggcaa agtcgccaaa
     8461 gacctgcaga aacgcgacgg cttgatcgag atttatgacc agatcatgaa tcagaaggtc
     8521 atcaagctgc tcgaagacaa cgcccggatt gaggacgtgc agcccgtcac gccgctgccg
     8581 gccgaggcgc ccgccgcccc gccaggctga gccatccgca tgcggcgggc aaccggggga
     8641 aagcgcgccc gccgccggcg tgagcgcaag ccggggcggc cagcgcggcg cgttattgcg
     8701 ttcgcccgaa cttcttttcc agctcccgga aactcatctc gatgagcgtc gggcggccat
     8761 gcgggcaggt gtacggcatg gcgcagtggc gcaaatcctc gaccaggttc tccagttccc
     8821 gcccggccag cgggtcgttg gccttgacgg catggcggca gacggttttg gcgatggtat
     8881 cctcgcccag ccgcagggca ttcagctcgc ggccggcggc ccgcagttca tccaccagtt
     8941 cgatcacaaa ccggcgggcg tcgggcacct tcacaaacgg cggcagggca tccagcagga
     9001 acgtgcgctc gccgaattcg ctcaggccca cgcccagccg cgtgagcacg gataactgct
     9061 cccgcaaaaa accggcctcg cgcgccgcca gctccaccgt ctccggcagc agcagccgct
     9121 gggagggcgc ctggtcatgc tgctccagcc ggcggagcat ttgctcgaac aaaatccgct
     9181 catgcgccgc atgctgatcc agcaacacca ggccccggtc cgattccagc accacgtaga
     9241 gccgcccaat cacgccaacc agccgcagcg gaaccttgag caggggaacc ggctccggca
     9301 gcggcggcgg cggcaggggc ccgggggcgg tcggcgggag aacggccggg gtggcgggcg
     9361 gtgcgggcgc cgggctgaag cccatgcgca ggggaggcgt cgcggaagcc gttagcggcc
     9421 gcggctgcgg cgcgggcgcg agggccgcgg ggaagttggg cagcgccgat tgcgcggcgg
     9481 gcggcggcgg gggcgcgagc ggcggcgggg cggccggttc gggcgggccg ggcgcggccg
     9541 cgggcggcgc ggcggacgcc ggcgttccgg agtgaaaggc cagcagcgtc tgccggattg
     9601 cctgcgccac cagccgccgc acttcggctt cccggtggaa tttcacctcc cgcttggcgg
     9661 ggtggatgtt cacatccacc atcgccgggt caatctccag gaagaggcag gcgaccgggt
     9721 agcgcccttt catgagggcc gtgtgatagc cttcgagcag cgcgaagttc agcccgcgat
     9781 tctcgatggg ccgccgattg acaaaaaggt gctgatcctc ccggttggaa cgggagacgc
     9841 ccggcgcgcc aatgaagccc caaaggcgaa acggggcggc cggggccggc gggcgggccg
     9901 gagccggggc cgtttccgcg tccggatcgt ccggcggctc cagcgcggcg gaataatcca
     9961 cccgcagcaa ggcgcgctcg ccgccataga ggatgcgcag gcgttcctgg agggcgccca
    10021 ggcgcgccgc ggagccgtcc ccggccttga cgggcggcag ttgccagacc gggcggccgt
    10081 cgcgctgaaa ggcaaacgcc acttcgggat gagccagcgc cgccagggtc aggtaatgct
    10141 ggatgtgggc ggattccgtc tcctcggtgc gcaggaactt ccgccgcgcc ggcaggttga
    10201 agaaaagctg gcgcacctcg accgtggttc ccggcgcgcg gcccgcggct ttgacctcca
    10261 ggatccggcc gccgtggacg atgatttgcg tgccctcggg cgaatcgccc tcgcgcgcgc
    10321 gggtggtgag ggtgaaccgg ctcacgctgg caatgctggg cagggcttcg ccgcgaaatc
    10381 ccagggtggc cagcgccgcg aggtcttcgg cccgccggat cttgctggtg gcatgccgct
    10441 ccaggcacag cagggcgtcg tcgcgcgcca tgcccaggcc gtcatccccc acgcgcacca
    10501 ggctgcgccc gccggcctgg atttccacca ccacccggcg ggcctgggcg tccagcgcat
    10561 tctcgaccag ttctttgacc acactggcgg ggcgctcgat gacctcgccg gcggcgattt
    10621 ggttggccac ttgctccggc agcaggcgga tgcggttcat ggcgttgccg gcgcggggcc
    10681 ggaaagggac gccgccgccg ccgcttccgg cgggcgccgg ggcaggccga aggagaacac
    10741 cgcgccgccg tccggcaggt tccgcgccgc gatccagccg ccgtgatccg tgagaatccg
    10801 cttgcagatg aacagcccca ggccggtgcc gtgcgccttg ccgtgcgtcg cgaacacctg
    10861 gaataactgc ccggccatct ccggcgcaat gcccggcccg ccatcctcca gttcggtcac
    10921 cacttccttc gcggtgacct gaaagcggaa aaccacccgg cctccggacg gcatggcgtc
    10981 ggtcgcatta tgcacgaggt tgtagaaaac ccggcgcagc cgtttggggt gaataaacac
    11041 ctcgacggag ggcggcgggt tcgccagcgc cagcgccacg gacttcaggc cggcttccgg
    11101 gcccaactcc tccaggagct gcgccacgaa cgcgccgtag ttgaccggcg ccagcacgaa
    11161 attcgcgggc gcgccctggg tgaaatcgag aatctcgttg atcagatcgc tgatgcgctc
    11221 gacctgtttg cggatatgcc ccgcggcctg ctgccgcgct ttcagggagg cccgcttctg
    11281 gccggcgacc tcggccgtca ggccgatgat attgagcggg tttttcaggt cgtgcacgat
    11341 ggagcgggcg aagcggccca cgatggccag gcgctccgcc tgcagcactt gccgcaggta
    11401 ttgctggtta aactcccgca gccggcggct gatctcctgc agcaacgcca gcgccagcgc
    11461 cggcgaccgc gccaccagcg ccagcatctc gtcgcggggg atgaaataaa cccgggtgtc
    11521 ctcccgggcc accgcccagg ccgagcgcgg ctggttctcg acgaccgcca tctcgccaaa
    11581 cacttccccc ggccccacct cggagaaaac cagccgcgcc cccgagtcca ccgaccccga
    11641 aatctccacc cgccccgacc gcaccacata caggccatcc ccgcgatcgc cctcgcggaa
    11701 aatctcctcc ccggcggcaa acgaccgttc ccgggcctgg cggctcagcg cgcttaactc
    11761 cgacgggcta agcggcgaga agagtttgct ggtttcgagc gcaaccacac acgcacttta
    11821 ggtaaaagcc ggcccaggta aagccatttg cggcgggggc ggcgggtcag ggaaactccg
    11881 gctttggcgt cccggcgcgc gggggaaacg cttccccggg gggaacgccg tcccgcgccg
    11941 ccgcgcttag ctctccaggt aattgagcgt ggtcttttcg atcggcaccc gggtgacgcg
    12001 gttcacattc cgaaacgcaa aggccagctc catatccttg agccgccagg tctcgataaa
    12061 ctgcagcgcc cggtcaatca gcttgaagtc acgcgccacg gcgggatcgc tggtccacat
    12121 gccaggtccc tggaagtaaa gccccgttgc cgtgtttctc aaaagcgttc tcatgtccgg
    12181 gtgggcgagc cactcaatat atctgccccc tatcaaagca ttaccggtgc cacccgcgtt
    12241 ttgggcctga attgggcctt tttgcccgct cacggcggct gtcaggcacg aagttcactc
    12301 cccggcgcgt gaaaataacg caaggcgctc ccggcgcccc ccatacgcgg cggacgggtg
    12361 gcgcgccggc gccgctcagc cccgcctgcc tacgcgctgc cgggcttgac ggcggggagg
    12421 gcggccaggt cgtccggaag ctgatccggc gcaaaggttt ccacttgcag cgtgaccagg
    12481 ctaaacaggg gcacgccaaa attaacctcg ccgcctgagc gatccacagc caccaccacc
    12541 ccgacgaccc gcccctggtg ggcgcggacg atgtctaggg tttcctggac gcgcccccct
    12601 ttggtaatca cgtcctccac aatcaaaatc ttctctccgg gcgcgatccg gaagccgcgc
    12661 cgcagcgcca gcttgccctg ctctttttcg acaaaaatga agcgccgccc aagctgccgc
    12721 gccacctcct ggcccagcac cagcccgccc atcgccggcg cgaccaccgt cgccgccccc
    12781 agcgcgccca cccggcgcgc cagttcgccc cccagttttt caacgatggg catttgctgc
    12841 aaggccagcg cgcattggaa gtactggcgg ctgtgccgcc cgctgcgcag cacaaaatgc
    12901 ccttccagca gcgcgccgct cgcgcggaag atctgcaaaa cttcactttc ggtcatggcg
    12961 gcatggtagg cgtccggccc ggcctgcaaa gcgcaaagtg gcttcttttt cgcccgcgct
    13021 tatgcttcac tccgcccgtg cccaagtggt gcaaaaccat cctggcgatc ctgctgctcc
    13081 cggcctgcgc cggggcggtg gcggcgttgg gccagctcct gcgcgccgcc ggcggcgcgg
    13141 accggacctg ggtgccgttt ctggccggcg ccgcctgctg gatcacggtt ttcctgctgc
    13201 tgcccaagcc catgcggctc tacgtcttcg ggcatgaact cacccacgcg ctgtggacct
    13261 ggctgttcgg gggccgcgtc aaacgcttcc gggccaccgc ccgcgggggg cacgtcgtcg
    13321 tcaacaaaaa caacttcgtc atcgcgctgg ccccgtactt cttccccctc tacgccgtgc
    13381 tggtcgtgct ggggttcctc ctcgggcatg cgctgtggga ttggcggcac tacctggccg
    13441 ggttccacct gctgctcggc gccgcctacg cctttcacct caccttgaac tggcatatcc
    13501 tgcaatttga ccaatcagac atccgcgacc agggctattt attctcggcg gtcatcatct
    13561 tcctcggcaa cgtggcggtg ttgctggtgg gaatcccgct gctggtgccc cgcgtgggcg
    13621 tcctcaccgc gctcggctgg tggctgcgcg gcaccggcga agtcctggcc cggatcgggc
    13681 gaatcctctg attccggccg ccgccgcccg gccgcgcccg gaaaatcttt ttccccgcgc
    13741 gagttgcgga tatgaattcg cgcttgtaat gtttcggggc ttcaggcgaa atgctggcat
    13801 agataggttt gagccgtatg gatttagccg acataataac caaagagcag atcattaccg
    13861 atttgcgcgc caccaaccgc tgggaagcga ttgatgaact gaccgataac ctggtggcaa
    13921 ccggtaaaat cagcccgccg caccgcgaag ccgtcatcgc cgcggtcaag aagcgcgagg
    13981 cgtccatgag caccggcatc ggctttggcg tcggcatccc ccacgcttcg accgatttga
    14041 tcaccagcgt ggtcgccgcg ctggggcgct cccggaaagg ggtggacttc gattcgctgg
    14101 acaaccagcc ggtgagcctg gtgctgctct ttctggtgcc ccagggccag tttcagaaac
    14161 acctgcacac gctggccaac atcgccaagc tgttgcacaa cgccgatttc cgccagaccc
    14221 tcgaacaggc ccccgacagc gccgccatgt tccaggcgat ccgcagccac gggctgaagt
    14281 aagtgtcccc ccgcgcgacc aggctgccgg gcgctcccgg gaaacgctcc cgccggcccg
    14341 ccggcaaccc ggcccgcccc caacccgctt aacccgtgct ccccgcccaa ctcatcgaac
    14401 aacgcctcca ggcggcggtg aaaaccgttc tgccggacgc ggacaccgcc gccgtcctgg
    14461 tccggccctg ccccgatccc cgcttcggcg attaccagac caacgccctc atggggctgg
    14521 cccgggcccg gaaactgaac ccgcgccaac tggccgccga cgtgctggcc cggctggacg
    14581 tggcggactg ctgcgagccg ccggaagtcg ccggcgccgg cttcctgaac ttccgcgtcc
    14641 agaccgccgc cctggcgcgc gcgctgcaag ccgccgcgcg gggcgagcac ctgttcttcc
    14701 cgccggcggc ggccccgcgc acggtggtgg tggatttcag ctcgcccaac gtcgccaaat
    14761 ccatgcacgt cggccatatc cgctcgacca tcctgggcga ttccctggcg cgcgtcctgc
    14821 ggctgctggg gcaccgggtc atcacggata atcacctggg cgactggggc acgcagttcg
    14881 ggatgctgct gacggcctgg aaacgcgacc tgaacgccgc cgcgctgcaa accgacgcga
    14941 tcgccgagat ggagcgcctc tacaaacagg ccgccgccga atgcaaaacc agccccgccg
    15001 cgctcgaagc cgcccgccgg gaactggtcc ggctgcaggc cggcgacgcg gagaacctcg
    15061 ccatctggcg ccggatgatc gcgctctcgc gcgcccagtt cgacatcatc taccagcggc
    15121 tcggcgtgcg gttcgaccac accctcggcg aaagtttcta ccacccgcgc ctgcagccgc
    15181 tcgtccagga attatgcgac cggggcctcg cgcgcgaaag cgagggcgcc cgggtggtct
    15241 tcttcgacga cctgcccgcc ttgcaagcgc atccggccct gatccagaag cgcgacggcg
    15301 ccttcaacta caccaccacc gacctcgcca ccctggccta ccggctcgag acctggcagc
    15361 ccgacgaaat catctacgtc accgacgccc ggcagcagct ccacttccag cagctcttcg
    15421 cggccttccg gcgctggcgc ccggaagccg ccgtgcggct ggcccatgtc tggttcggct
    15481 ccatcctggg cgaggacaac aagccgttca aaacccgctc gggcgaaacc gtcaagctgg
    15541 ccgacctgct ggacgaagcc gaggaacgcg cctaccgggc ggtgtccgaa aagaacccgg
    15601 ccctggccga agccgaccgg cgcgaggtgg cgcgggtggt gggcctgggc gcgctcaaat
    15661 acgccgacct gctgcccaac cgccagagcg attacgtgtt cagttgggac aaaatgctgg
    15721 ccctgaacgg caacaccgcc ccctacctcc aatacgcctg cgcccgcatc cgcagcatct
    15781 tccgcaaagg cgacgtgggc ggggacctcg cgcccgccgc cctggagctg ggcgcgcccg
    15841 aggagctggc gctcgcgcgg cacctgctca actttggcct ggtgctggcg gccgtggccg
    15901 aggaataccg ccccaacttc ctctgcaatt acctttacga actggcgggc cggttcaccg
    15961 ccttttacga gcattgcccc gtcctcaagt ccgcccccgg cccgcgcgcc agccggctgg
    16021 tgctctgcga cctcacggcg cgcgtcctgc agcagggcct ggcggtcctc ggaatcgaga
    16081 ccctcgaaca aatgtaagcc cgtcaacgcg gacgggattt cccgccgccc gcccgcccca
    16141 cgcccgggag cgccaggtac tgctgcgcgt tggcgtagca gatgtcccgc accatcggcc
    16201 cgagccgctt caggtctttg ggcagctcgc cgttctccgc gtcccgcccc accaggttgc
    16261 agagcacccg ccggaaatac tcatgccgcg gaaaggacat gaacgaccgc gagtccgtca
    16321 ccatgccgac aaagcgggac agcagcccca ggttggacag cgcgttgagc tgccactcca
    16381 tcccctcctt ctggtccagg aaccaccagc ccgagcccat ctgcaccttg gccgcccgcc
    16441 catcgcccgg aaaattgccc gtcatcgccg cgaaaaggta gttatccgcc ggattgctgt
    16501 tgtagagaat cgtccgcggc agcgcctgct cgcgatccag cgcgtccaga aacgccgcca
    16561 gcgccacgcc ctgcggataa tccccaatgg aatcaaagcc cgtgtccgga cccaactgcc
    16621 ccagccgccg ggtattggta ttgcgcagcg ggcccaggtg aagctgcatc gtccagtcct
    16681 tcccggcatt gagccgcccg aaaaaccgca tcagcaacgc gccgaaccgc gcctgttccg
    16741 ccggcgacgc cgcccggccc cgccgcgccc gggtgaagat cgctcccgcc gtcttgagcg
    16801 tggtgtcctc cgtataacac cgggccaggc cgtgatccga caaccggcag ccgcacgcat
    16861 gaaaataatc atgccgcttc tccagcgcct ccaggaacga tcccagcccc cgaatctcca
    16921 cattcgccgc cgcttccagc cgctgaaccc actggttgaa cggaaccggc tgatgcaccg
    16981 ccagcccccg gtccggccgg aacgtcggca agacccgcgt cgccaggccg gacgcggcca
    17041 gcgcccggtg atgcgccagg tcgtcggtcg ggtcatcggt ggtgccgacc acggccactt
    17101 tgaaccgccg cagaatcccc caggcactca accccggcgt ggccagccgt tcggcggcct
    17161 gcttccagat gcgcgccgca cttcgctcct cgagcggctc gtcaatcccg aagtagcgct
    17221 tcaattcgag gtgcgtccag tggtaaagcg gattgcgcag cgtgcgcggc accgtggccg
    17281 cccaggcctt gaacttctca aacggcgaag cgtcgcccgt gcagaaacgc tccggcacgc
    17341 cgttggcgcg catggcccgc cacttgtaat gatccccctc aagccaaatc tcaaagagat
    17401 tggcaaactg ccggttccgc gcgatgtccc ccggcgaaag gtggcaatgg taatcaaaaa
    17461 tcggctcgtc cgccgcatat tgatgataca gccgcgccgc cgtccgggtc tggagaagaa
    17521 agtccgggtg aatgaagctc atacggcggg aattataaac cccgccgccc cgccggagca
    17581 agcgctcttg gcatttccca aaaatcagcc gcggctcctc agccagtgat aaggactgga
    17641 gccaaattca atcccccaac gctttcgaca tagccggaaa ccgctcccga tatacaagcc
    17701 gttctcccca gccttcactg tccaattatt ggacactccc ccaatccctc acgttaacgt
    17761 ccatcctgcc gatttttcca acatcccccg tatttttaca caaccccaat catttcagcc
    17821 tgatccatac ctaacaaccc gctgtgtaca aggggtgtac aagggatgct catcgggtgt
    17881 accagctaaa atccgatgga ctcccggtga gcatcccttg tacacccctt gtacacggcg
    17941 cgaatagatg gggaaagcag gcagtgttct caggaatgag aatgccggat gggagggagt
    18001 tgggcggggg cgatagagat tggggtgggg gaggcaggaa cctgtcttcg ctttttcggg
    18061 gataccgggt ggatttttct tgaagggaca gccgggcatt
//
LOCUS       DAOPQG010000075        18089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3718_length_18089_cov_19.273428, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000075 DAOPQG010000000
VERSION     DAOPQG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 18089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18089
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3718_length_18089_cov_19.273428"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2433)
                     /locus_tag="PKI07_09465"
                     /pseudo
     CDS             complement(1..2433)
                     /locus_tag="PKI07_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524554.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(2430..3434)
                     /locus_tag="PKI07_09470"
     CDS             complement(2430..3434)
                     /locus_tag="PKI07_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HOC51319.1"
                     /translation="MTGNQQFLLYTAPDGAVKVEVFFKDETVWLTQKALAELFGVKVP
                     AINKHLRNIFASGELVREATVSKMEIVRPEGAREVAREVEFYNLDAIIAVGYRVNSYQ
                     ATRFRIWATKTLREFIVKGFVLDDERLKQGKRVFGKDYFDELLERIREIRASERRFYQ
                     KITDIYALSADYDPQAPITKDFFATVQNKLHWAITGQTAAELIYSSADAAKINMGLAT
                     WKNAPHGKILKSDVAVAKNYLNEAHIKELNRIVSAYLDLAENRAERGIVTRMAEWVEF
                     LNRFLELSNYPILQDKGKVSALEAKLKAETEYEVFRQRQDREYISDFDREIKRLEGKK
                     "
     gene            complement(3431..5014)
                     /locus_tag="PKI07_09475"
     CDS             complement(3431..5014)
                     /locus_tag="PKI07_09475"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOC51320.1"
                     /translation="MGGDGWRTARLVDVAQINPDSIGRGWPHTQIHYVDISSVGEGQI
                     QVPPQLLALADAPSRAKRLIRSGDTVLSTVRPNRRSMFFADQPGPDWVVSTGFAVLRP
                     QVQMIAPRFLYACVFSQSFTEYLVSQEKGAAYPAVSPEDIGNAVIPLPPLKEQQAIAC
                     ILGALDDKIELNRTMNRTLEATARAIFKSWFVDFDPVHWNSARRLANIPRSEPGKWFI
                     YAIECEGGSHYIGFTDDLHRRFDEHCRGCGADWTKAHPPVRLAYWEPVDSQAEAVARE
                     QKLKSGSGREWLKAEIARNWTPRRPLPAGRQVKPEIAALFPDSFEPSELGEIPKGWRV
                     DSLDGIANYLNGLALQKYPPKNAGTLPVIKIAQLRKGDSYGADLCSANLPTDYIIQDG
                     DVLFSWSGSLEVEIWCGGAGALNQHLFKVTSHRFKKWFFFLWTLHHLNDFRLIAADKA
                     TTMGHIQRKHLSDARVLIPSKPLMDSMDNLMDPLVERVVSCRLESRTLAALRDTLLPK
                     LISGELRIPDAERIAGRYL"
     gene            complement(5004..6551)
                     /locus_tag="PKI07_09480"
     CDS             complement(5004..6551)
                     /locus_tag="PKI07_09480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOC51321.1"
                     /translation="MTETTNQGANLGFECKLWRAADAMRSNMDAAEYKHVVLGLIFLK
                     YISDAFEEQHAKLDADRAKGADPEDRDEYIAQNIFWVPREARWSNLKANAKQPAIGKV
                     VDDAMLAIERDNPSLKGVLPKDYSHPRLDKQRLGQLIDLIGNIGLGDKANRSKDILGR
                     VYEYFLSQFASAEGKKGGQFYTPRCVVGVLVEMLAPYKGRVYDPACGSGGMFVQSEKF
                     VEAHGGKIGDISIYGQESNHTTWRLAKMNLAIRGIDGNLGKEHADSFHRDLHPDLKAD
                     YVIANPPFNDSDWGGERLKDDKRWKYGIPPAGNANFAWVQHFIHHLAPTGLAGFVLAN
                     GSMSSNQSGEGEIRKNIIEADLVDCMVAMPGQLFYSTQIPVCLWFIARNKSNGQFRKR
                     TGQTLFIDARKLGTMIDRVHRELTEEDIQRIADTYHAWRGDKSAGNYEDIPGFCKSAT
                     LDDIRHHNHILTPGRYVGAAEVEDDGEPFEEKMARLTTALREQSAQAEKLDQIIWANL
                     EDIGYGG"
     gene            complement(6898..10167)
                     /locus_tag="PKI07_09485"
     CDS             complement(6898..10167)
                     /locus_tag="PKI07_09485"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC51322.1"
                     /translation="MPRNGNGSRASQVSKYDAQNTALTKTYGALADLRRELGDSSAVV
                     AEREKLLRVFAGCADSQRAWLLLEDYFEKLRLSRKDFPNSDWWPRLLAVQGTARLEEL
                     AFLFLRARRPVPTELSVYANRDRFERLEQAAREQQLVRHLETWLFPEPPAHFDAPRAF
                     VRAICHPQPDPEQPACCRLAVELRLARPRTGEKVRTLGEILELTTRAAHEQELFPPGD
                     WEFIRWLAETHSHRRNGETPLVLSDLELLHWLARWGHTARLELALGKEPPQPLEFQGA
                     VAELTPHLENGGAGLAFTHRLTVPGGGIYPLSEVRFFNRHPTLALAGRTFYLLRSAPP
                     PPVFELWNTQPALPLAKLSHRLLLHLRKTQSRQGVDWEQLCLSHAAKPQFLFELLAET
                     VRLQLRARSQRDQSLWFWNGHEWKRHASNPPRPDGARPELLDDPRLEPATQWLRRLDW
                     FTPEPGLWVGDANETFLGLLARAWADRPAEADYLGNPGFSRLFLAPRQLRPRLVVKGS
                     GIDWLEVSAEWEQEGLKLTAADLQRLQAATGRFVKLPDAGWVELDADAVHSAHEAMAD
                     LGVEGLAAAPQRLGLEQAAHLGEDGWRRFCDSAAARALRERIRNFTGLPAMPLPAAVR
                     AELRPYQKAGFDFLCHLTRLRLGGILADDMGLGKTLQTLAWLAWLKDQHRKDPQPALV
                     ICPASVLHNWRREAERFTPQLRVLVLESGAARHNLRRQVPQYDLIVTNYALLRRDLEE
                     LQKFAFRAIVLDEAQFIKNPEAQVTQSVKQLKAAHRLALTGTPLENRLLDLWSIVDFI
                     QPGYLGRQDRFTETYEPRGEESEAAQRIARRRLSARLRPLLLRRLKQQVAKDLPERIE
                     QRRDCQLGDAQRKLYLAELRRSRELVLKTVAEQGLNKSKIHVLAALTRLRQICCHPRL
                     VGNDAASGKTETLFELLDALSAEGQKVLLFSQFVQMLHLLEAECRQRQIPTHSLTGQT
                     RDRAAVVSAFQNDPRPAVFLLSLRAAGTGLNLTTASYVVLYDPWWNPAVEAQAIDRSH
                     RIGQTRTVNAYRLISPGTVEEKIWELQQRKARTIADVLGEEGFARSLSQTDLEYLFAE
                     A"
     gene            complement(10311..11366)
                     /gene="hypE"
                     /locus_tag="PKI07_09490"
     CDS             complement(10311..11366)
                     /gene="hypE"
                     /locus_tag="PKI07_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOC51323.1"
                     /translation="MTTPPNFTLSCPFPIQDYPHVLMAHGGGGKLMHQLIGKLFVPAF
                     RNSLLEAQHDASVFDLAGQRIAFTTDSYVVRPLFFPGGDLGSLAVHGTVNDLAMAGAR
                     PRYLSAAFIIEEGLPMETLWKITASMQQAAQAAGVQIITGDTKVVDKGKGDGLFINTA
                     GIGILEHSLRIAPQSVQPGDAILVSGDVGRHGMAIMAVREGLEFESAIRSDSAPVAEA
                     VLQLIQAGLEIHCLRDLTRGGLTSVLNEIAEAARLTLQVEEKLIPVREDVRAACEILG
                     LDPLQVACEGRFAVFVPERDAARALEILRAHPVSAGAARIGQASPQSTARVLLQSAIG
                     ARRILDMSSGEQLPRIC"
     gene            complement(11363..12451)
                     /gene="hypD"
                     /locus_tag="PKI07_09495"
     CDS             complement(11363..12451)
                     /gene="hypD"
                     /locus_tag="PKI07_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOC51324.1"
                     /translation="MKYLDEYRDAQGAHKLARELARITTRPWSLMEVCGGQTHAIVKF
                     GIDELLPSQVTLIHGPGCPVCVTSLELIDKALEIAARPGVIFCSFGDMLRVPGSTTDL
                     LAVKAAGGDVRITYSPLDAVKLAEQNPAREVVFFAVGFETTAPATAMAVYQAARKGLK
                     NFSLLVSHVLVPPAIEAILGAPDRRVQGFLAAGHVCAVMGWEEYKPIAAKHRVPIVVT
                     GFEPLDILQGVLMCVRQLESGRAEVENQYSRAVRQEGNRPAQQIIREVFQITPRKWRG
                     IGEIPRSGLGLRDAYASFDAEHKFGVAGLRVDEPTECLSGLVLQGKIRPRECPAFGTR
                     CTPEHPLGATMVSSEGACAAYYRYRREP"
     gene            complement(12448..12741)
                     /locus_tag="PKI07_09500"
     CDS             complement(12448..12741)
                     /locus_tag="PKI07_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOC51325.1"
                     /translation="MCLAIPGKITSISGEDPLLRTGKVDFGGILKEVSLAYVPEAKLG
                     DYVIVHVGFALSRVDEEEAKQVFEYLRQMQELSELQESEAGVAPAPAPGPPPQ"
     gene            complement(12813..15218)
                     /gene="hypF"
                     /locus_tag="PKI07_09505"
     CDS             complement(12813..15218)
                     /gene="hypF"
                     /locus_tag="PKI07_09505"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOC51326.1"
                     /translation="MGSSVRLRIVVRGAVQGVGFRPFIYRLAVESGLAGWVSNSAQGV
                     FIEVEGPRAALEKFRLRLPAEKPPRSSIQSLEAAWLDPAGYAGFAIRPSDPGGARTAL
                     VLPDIATCADCRAEIFDPRNRRYGYPFTNCTHCGPRFSILESLPYDRPNTSMKAFVMC
                     PQCQAEYDDPRDRRFHAQPNACPACGPRLELWDRSGKPLPAATAPAGAPIAAAAEALR
                     QGRIVAVKGLGGFLLMVAAHNEDAVCRLRALKHREEKPFALMFPSLAAIRAECEVSPL
                     EERLLQSPEAPIVLLRRARAPRPAPSALPLAPSLAPHNPNLGVMLPYAPQYHLLLAAL
                     GFPVVATSGNLSDEPICTDEYEALDRLGGIADLFLVHNRPIVRHVDDSIVRLMSGREL
                     VLRRARGYAPLPIQLPVSLGGPRSAPGHSVLAVGAHLKNAIALSVGPQVFIGQHIGDL
                     ETDLAFAAFRRVIADFQTLYDARPSIIAADAHPDYLSTQFAHELAARGRHGEEAQTPP
                     PQLLTVQHHLAHVFSCLAENELAPPVLGVSWDGTGYGLDGTVWGGEFFLVTETACARA
                     AHLRPFRLPGGDSAAREPRRAALGLLYEALGEAAFAQTHLAPLRAFTPAELAPLKTML
                     ARRINSPLTSSVGRLFDAVAALTGGRQRAHYEGQAAMELEFALPDTASADEAYELPIR
                     SEPEARSSSRAPLILDWAPMLEAILADLDRGVPVSQISARFHQGLVEAIIAVARRLGQ
                     PRVVLSGGCFQNRCLTEHAVRRLREAGFRPYWHQRVPPNDGGLALGQIVAALSPNARL
                     LPS"
     gene            complement(15304..16797)
                     /locus_tag="PKI07_09510"
     CDS             complement(15304..16797)
                     /locus_tag="PKI07_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51327.1"
                     /translation="MPDPNNPPSPGPLRSFLGKFTVLFGAVRELWIVFAIVLFSNLAY
                     RVVNFTLRLWLSSDLGYNDVQSGLVVLLWSAGLTLCIVLVGSLTDALGLRKTFLLGFA
                     LCIISRVFITFTTVKWLALGVGLTVLALGEGMGAPVTIAALRRYTTTAQRSIAFSIFY
                     AIMNAGFLIGVLIFDALRAKLGEHDGFTLPGLGMQLSTYRAILLASLALSIPSFLIIY
                     FWLREGVEATDQGVVITPEQVKYPGQNALRVLRLTIGDTARDTARIFAGLWRQTGFYK
                     FLAFLSLAVFIKMIFVHMDYTYPTFGIRELGEGAPVGHLYGLNNILIVFLAPLVGVLT
                     QRISAYRMVIFGSFIAAASVFIMAVPPQWFRPLADGWFGHGIAHVWLGLSGTVNPYYV
                     MIFLFVLFLSIGESFYSPRLYEYTAAIAPKGQEASYMAMSSLPFFLAKIGVGPLSGVL
                     LDRFCPATGPRHSGTLWLIIGLSTLIAPVGLFVFQRFIRVHEAGRDD"
     gene            16928..17689
                     /locus_tag="PKI07_09515"
     CDS             16928..17689
                     /locus_tag="PKI07_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51328.1"
                     /translation="MTGILYGPQSGGFRKQPVASPPGFRREGGRAFTLIELLVVLAII
                     GLLASLLLPALARAREASLRVKCANHLKQLGYAIRMYADDSNGRYPMRALAGYWPAAL
                     LEYYQSTNILLCLSDRRRGPPQTDFAAQLAADRANRSYLINGWNDYFGTALNAASAMR
                     EAAVIYPAETIVFGEKKNSVAHYFMDLCEDHGNDLDSVEQACHSASQKSVYSGGANFT
                     YADGSVRYMKYGTTVWPLNLWAVSTTNRLLFAWQR"
BASE COUNT         3247 a         5562 c         5978 g         3302 t
ORIGIN      
        1 agaaggccac gtcgtcccga atctccagcg ccttctcatg cggaacggcc agcgcgaacg
       61 ccttcgacaa atcgctgacc gccttcacaa accggttctt gccgtcttcc tgggccagca
      121 catgttcttg cgccgctggc agtatcgaga ggcgttcctg cggtccgccg gtcttccatt
      181 gcgtccagtc gaacccgtgg aagatgccgc agcagacctc gtaacgttcg agcatgaccg
      241 cgacggcttc cgcttggtca atcgtcgtct gccccttgcc gccgctctgg gtgtagttcg
      301 ccagggcgta cttgagctgg tcggccagcc ccagatagtc cacgaccagc ccgcctggct
      361 tgtccttgaa aaccctgttg acgcgggcga tggcctgcat cagcccgtgc ccgcgcatcg
      421 gcttgtccac atacatcgtg tgcaggcacg gcgcatcgaa gcccgtcagc cacatatcgc
      481 ggacgatgac cacgttaaag gggtctttcg ggtccttgaa ccgtttggcc agttcctcgc
      541 ggcgaggctt gtttcgaatg tgctcctgcc actctgccgg gtctgaggcg gatccggtca
      601 tcacgatctt caggttgccc gcgttgtcat cggcgtggtg ccagtccggg cgaatcgcgg
      661 cgatggcctt gtagaggtcg acgcagatgc gtcggctcat gcagacgatc atcgccttgc
      721 cgtccatcgc ctccagacgt cgctcgaagt gctttaccag gtcctcggcg attagcttca
      781 atcgcttctc cgtgcccacc agggcttcga gttgcgccca cttggtcttc agccgctcct
      841 tgtgctcgac ctcctcgccc tcggtggcct cctcgaactc ctcgtccagc ctcggccgct
      901 cagtctcttt taactccaat ttggccaggc ggctctcgta gtagatcgga accgtcgcgc
      961 cgtccttcac cgcacgcttg atgtcgtaga tgctgacata gtcgccgaac accgcccgcg
     1021 tgttcttgtc ggtcaattcg ataggggtgc ccgtgaatcc gatgaaagac gcattgggca
     1081 gcgcctccct catgtgccgg gcgaaaccgt caatgaagtc gtactggctg cggtgcgcct
     1141 cgtcggcaat gacgacgatg tttcgccggt cggagaggag tgggtgatcg cccccgtcgg
     1201 aggggaagaa cttgtgaacc gttgtgaaca ccacgccgcc cgatgccgtt gtcagcaact
     1261 cccgcagatt cgcgcggcta tgtgcctgca cgggctgctg cggagcagct cgtggcagcg
     1321 ggcgaacgta ccatagagct ggtcgtcgag gtcattgcgg tcagtgatca cgacgagtgt
     1381 cggggtctcc attgccggat gcaggaccac ccgcccagcg tagaaggcca tcgtgaggct
     1441 cttgccggaa ccttgcgtat gccagacgac gcccacccgc cggtcgccgg gtttggcatc
     1501 gggatgtccc ttggcgaagt aggtgccttc gccttctcgc gcctgcaagt gcctttcagg
     1561 gggaggcagg cacgcccgga cggtctcttg tactgcgaca ttgaccgcgt ggtactggtg
     1621 atagcctgcc atcttcttaa ccaatacacc accccccaca tcctcaaaga cgatgaagtg
     1681 ccggatcagg tccaggaagc gacgcttctc aaacacgccc tcaatcacga cttgaagctg
     1741 aggcagccgg ttgtctgcca attcttcgcc ttcaatagtc cgccagggca tgaaccactc
     1801 gcggtcggcc gtcagcgtgc cgatccgcgc ctgcccgccg tcggagatga ccagtgcctc
     1861 gttgaaggca aacagtgagg gaatctgccg cttgtaggtc tgaagctggt tgaacgccga
     1921 ccagatggtc gcgttctccg tggcggcgtt cttcagctcg atcaccgcga ccggcagacc
     1981 gttgatgaac agcacgacat cgggacgccg ttcatgctgg tcctccacca cggtatactg
     2041 gttgaccgca aggaactcgt tgttctccgg attgtcgtag tcgaatatcc ggactagatc
     2101 gccgccaatc gaaccgtcag ctcgctggta ctcgacaggt acgccttcaa ccagatactt
     2161 gtgaatgacg tggttgttcg ccaccaatga cggcgagtcc ggccgcgtca acttgcggaa
     2221 ggcttcttcc aaggcatccg tcggcacctg cggattgagc cgcgccagcg cctggcgaag
     2281 ccgatattcc agcacaacct ggccgtagtt atcacgctcg gcggcaggct cccccggggc
     2341 aatctccggc cccgacagaa tcagatagcc cagactctcc agccacgcca aggcggcccg
     2401 ctcgatgtcg gattcacgga acgcgggact catttcttac cctccaatcg tttgatttcc
     2461 cgatcaaagt ccgaaatata ctcgcgatcc tgccgttgcc ggaacacctc gtattcggtc
     2521 tccgccttga gttttgcctc caaagcgctg acttttccct tatcttggag aatcgggtaa
     2581 ttggacaact ccagaaaccg gttcaggaac tccacccact ccgccattcg cgtgacgatg
     2641 ccgcgttccg cccggttttc ggccagatcg agataggcgg agacgattcg gttcagctcc
     2701 ttgatatgcg cctcgttcag gtagttcttt gccacagcca catccgactt taaaattttc
     2761 ccatgcggtg cgttcttcca agtcgccagt cccatgttga tctttgcggc atcggcggag
     2821 gaatagatca gttccgccgc cgtctgtccg gtgatggccc agtgcagctt gttctgaacc
     2881 gtggcgaaga aatccttggt aatcggcgcc tggggatcgt aatccgccga aagggcgtag
     2941 atatcggtga tcttctgata aaatcgccgc tcgctggccc ggatttcccg aatccgctcc
     3001 agcaattcgt caaagtaatc cttgccgaaa acccgtttgc cctgcttgag ccgttcgtcg
     3061 tccagcacga accccttgac gatgaactcc cgcagcgtct tcgtggccca aatgcggaag
     3121 cgcgtggcct gatagctgtt cacccggtag cccacggcga taatggcgtc gaggttgtaa
     3181 aattccactt cgcgggccac ctcacgagcg ccttccgggc gaactatttc cattttggaa
     3241 acagttgcct ctcgaaccag ttccccggat gcgaagatgt tcctgagatg tttgttgatc
     3301 gcaggtacct tcactccgaa caactccgcc agggcctttt gcgtcaacca gaccgtctca
     3361 tccttaaaaa agacctcaac cttaaccgcc ccatccggcg cggtatagag caggaattgc
     3421 tgattgccgg tcataaatat ctccccgcaa ttcgctcagc atccggtatg cgtaactcgc
     3481 cggaaatgag tttgggcagg agggtgtcgc gcagggcggc cagggtgcgg gattcgagtc
     3541 gacaagatac aacccgttca accaacgggt ccattaagtt gtccatactg tccattaaag
     3601 gttttgaggg tatcaggaca cgggcatccg acagatgctt acgctgtata tgacccatgg
     3661 ttgtggcctt gtcggccgcg attagcctga aatcgttcaa atggtgcaat gtccagagaa
     3721 aaaagaacca tttcttaaaa cgatgtgatg taaccttgaa aagatgctgg ttcagcgcac
     3781 cagcaccgcc acaccatatt tcgacttcaa gactgccaga ccacgagaaa agcacatctc
     3841 cgtcttgaat aatgtagtca gttggaagat tggcactgca aaggtcagcc ccatatgaat
     3901 cacccttgcg aagctgggct attttgataa ccgggagcgt ccctgcattc ttcgggggat
     3961 acttctgaag agcaagtcca ttcaaatagt tggcaatgcc atccaaacta tcgacccgcc
     4021 accccttcgg aatctcgccg agttcggaag gctcgaagga atccgggaaa agggcggcga
     4081 tttcgggttt gacctgcctg ccggcaggca ggggccggcg gggcgtccag ttccttgcaa
     4141 tctcggcctt gagccattcc cggcctgatc cagacttcag tttctgttcg cgggcgaccg
     4201 cttcagcttg cgaatccacg ggctcccaat aggccaagcg aaccggcggg tgggcttttg
     4261 tccaatcggc tccgcaacct cggcagtgtt cgtcaaacct tcggtggagg tcgtccgtga
     4321 acccaatgta atggctacct ccttcacatt cgatggcgta gataaaccac ttgccaggct
     4381 ctgagcgtgg gatgttcgcc aacctcctgg cgctattcca atggacgggg tcgaagtcca
     4441 cgaaccagct cttgaagatc gcccgtgccg ttgcctccag tgtccggttc atcgtgcggt
     4501 tcaactcgat cttgtcgtcc agcgccccca gaatgcaggc gatggcctgc tgctccttga
     4561 gcggaggcaa aggaatcact gcattcccga tgtcttctgg cgacaccgca ggatacgccg
     4621 ctcccttctc ctgtgaaaca aggtattcag tgaaggattg actgaagacg catgcgtaca
     4681 ggaatcgagg ggcaatcatt tgcacttgcg ggcgaagcac agcaaaaccc gtggagacca
     4741 cccagtcggg cccaggctgg tcagcaaaga acattgaacg tcggttggga cgcacggttg
     4801 aaaggacagt gtcgccgctt cggataagcc gcttcgctcg gctaggtgcg tccgcaagtg
     4861 caagcagctg aggcggaacc tgaatctgcc cttcgccaac tgatgaaatg tcgacataat
     4921 gaatctgcgt atgaggccag cctcgcccga tggagtcggg atttatctgt gccacatcta
     4981 caaggcgtgc tgttctccaa ccatcacccc ccatagccaa tatcctccag gttggcccag
     5041 atgatctgat cgagcttctc ggcctgtgcg ctctgctcgc gcagagcggt tgtcagtctg
     5101 gccatctttt cctcgaaggg ttcgccatcg tcttccacct cggccgcgcc aacatagcga
     5161 ccgggcgtga ggatgtgatt gtggtggcga atatcatcga gcgttgcgct tttgcagaag
     5221 ccgggtatat cttcataatt cccggcagac ttatctcccc gccaggcgtg gtaggtgtcc
     5281 gcgatcctct ggatgtcctc ctcggtgagt tcacggtgaa cgcgatcaat catcgtcccc
     5341 agcttgcggg catcgatgaa gagtgtctgg ccggtgcgct tgcggaattg tccgttgctc
     5401 ttattgcggg cgatgaacca gaggcagacg ggaatttggg tggagtagaa aagctgccca
     5461 ggcatcgcca ccatgcagtc caccaggtcg gcctcgatga tgttcttgcg aatttcgccc
     5521 tcgccggatt ggttggagga catcgagccg ttggccagga cgaagcccgc caggccggtc
     5581 ggggccaggt ggtggataaa gtgctggacc caggcgaagt tggcgttgcc cgcaggcggg
     5641 atgccgtatt tccagcgttt gtcgtccttg aggcgctcgc cgccccagtc ggaatcgttg
     5701 aagggcggat tggcgatgac atagtcggcc ttgaggtcgg ggtggaggtc gcggtgaaag
     5761 ctgtcggcgt gctccttgcc aaggttgccg tcgatgccgc gaatggccag gttcattttc
     5821 gccagccgcc aggtggtgtg gttggattcc tggccgtaga tgctgatgtc gccaattttg
     5881 ccgccgtggg cttcaacgaa cttctctgat tggacgaaca tgccgccgga gccacaggca
     5941 gggtcgtata cgcggccttt gtacggggcg agcatctcga ccagcacgcc gaccacacag
     6001 cgaggcgtat agaactggcc gcctttctta ccctcggcgc tggcgaattg ggagaggaaa
     6061 tactcgtaga cccgtcccag gatatccttg gatcggttgg ccttgtcgcc taggccgatg
     6121 ttgccaatga ggtcaatgag ctgtccaagg cgctgtttgt cgaggcgcgg gtgggagtaa
     6181 tccttcggca gcacgccttt gagggacgga ttgtcgcgct cgatggcgag catggcgtcg
     6241 tccacgacct tgccgatggc cggttgcttg gcgttggcct tgaggttcga ccagcgagct
     6301 tcccggggta cccagaagat gttctgggca atatactcat cgcgatcttc cgggtcggca
     6361 cccttggcgc ggtcggcatc gagcttggcg tgctgctctt cgaaggcgtc tgagatgtac
     6421 ttcaggaaga tcaggccgag gacaacgtgc ttgtactcgg ccgcgtccat gttggaacgc
     6481 atcgcgtcag ccgcccgcca aagtttgcac tcgaatccca ggtttgcgcc ctggttggtt
     6541 gtttcagtca tcacatctgc tccgcaattc gtgttggttc cagcgctgag tccgggccac
     6601 ccgtgccaag ccgcagtcaa ccgtccccgc aggcgattgt taacctttgg cacaatcttt
     6661 cgaagacggg tggaatgcta tcagttgggg taaaagcgcg caagcagaac caggctgctc
     6721 cggggcaatc tgatcactag ctgcgttccg gatcgttgcg ccgacgggct ggaaccggca
     6781 ggcggcagga ttcccgctcc acagtcacct ggcggcgtgg ccaaaccagg cgtttgggac
     6841 gggcgccggg cggatctctg gcgtgttcag gttggcggac cgggtcgaat ccgggcttta
     6901 cgcttcggcg aacaggtatt ccagatctgt ttgggagagg ctgcgggcga agccttcttc
     6961 gccgagcacg tcggcgatgg tccgggcttt gcgctgttgc agctcccaaa tcttttcttc
     7021 gacggtgccg ggggagatca ggcggtaggc gttgacggtg cgggtttggc cgatgcggtg
     7081 ggagcgatca atggcttggg cttcgaccgc cgggttccac cagggatcgt agagcacgac
     7141 gtagctggcc gtggtcaggt tcaggccggt gcccgccgct cgcaggctga gcagaaacac
     7201 cgccgggcgg gggtcgtttt ggaatgcgct cacgacagcg gcccggtccc gggtttggcc
     7261 ggtgaggcta tgggtgggaa tctggcgctg gcggcattcc gcttcgagca ggtgcagcat
     7321 ctgcacaaac tggctgaaca gcagcacctt ctggccttcg gcggagagcg cgtcgagcag
     7381 ttcaaacagg gtttcggtct tgccggaggc ggcgtcgttg cccaccagcc gggggtggca
     7441 gcaaatctgg cgcagccggg tgagggcggc cagcacatgg attttgcttt tgttgaggcc
     7501 ctgctcggcc acggttttca gcaccagctc gcggctgcgg cgcagttcgg ccaggtaaag
     7561 cttgcgctgc gcgtcgccca gttggcagtc gcgacgctgc tcgatgcgtt caggcaggtc
     7621 tttcgccacc tgctgtttga gccggcgcag cagcaacggg cgcagccggg ccgacaaccg
     7681 gcgccgcgcg atgcgttgcg ccgcctcgga ctcctcgccg cgcggctcgt aggtttcggt
     7741 gaagcggtcc tggcggccga ggtagcccgg ctggatgaag tccacgatgc tccacaggtc
     7801 cagcaggcga ttctccagcg gcgtgccggt cagcgccagg cgatgggcgg cttttaattg
     7861 cttcacggac tgggtgacct gggcttccgg gtttttgatg aattgcgctt cgtccaggac
     7921 aatggcccgg aaggcgaact tttgcagctc ctcgaggtcg cggcgcagca gggcgtagtt
     7981 ggtgacgatg aggtcgtatt gggggacttg ccggcgcaag ttatggcggg cggcgccgct
     8041 ttcgagcacc agcacccgga gttgcggggt gaagcgttcc gcctcgcgcc gccagttatg
     8101 cagcaccgag gcggggcaga tcaccagcgc cggctggggg tctttccgat gctggtcttt
     8161 gagccacgcc agccacgcca gcgtttgcag cgtcttgccc aggcccatgt cgtccgcgag
     8221 gatgccgccc agccggagcc gggtaaggtg gcagaggaaa tcaaagcccg ctttctgata
     8281 cggccgcagt tccgcccgca ccgcggcggg cagcggcatg gcgggcagcc cggtgaagtt
     8341 ccggatgcgc tcgcgcaagg cccgggccgc ggcggaatca cagaagcgcc gccagccgtc
     8401 ttcgcccagg tgcgccgcct gctccaggcc gaggcgctgg ggcgccgccg ccagcccttc
     8461 cacgcccagg tcggccatgg cctcgtgcgc cgaatgcacc gcgtccgcgt ccagttcgac
     8521 ccagccggcg tcgggcagtt ttacgaagcg tccggtggcc gcctggagcc gctgcaagtc
     8581 ggcggcggtg agtttcagcc cctcctgttc ccattcggcg gagacctcca gccagtcaat
     8641 gccgctgcct ttgaccacga gccgcgggcg cagttggcgc ggcgccagga acaggcggga
     8701 gaaaccggga ttgcccaggt aatcggcctc cgccgggcgg tcggcccagg cgcgggccag
     8761 caggcccagg aaggtctcat tggcgtcgcc gacccagagg cccggttcgg gcgtgaacca
     8821 atccagccgc cgcagccatt gcgtcgcggg ttccagccgc gggtcatcca gcagttccgg
     8881 ccgggcgccg tccgggcgcg ggggattcga ggcgtgccgt ttccattcgt ggccgttcca
     8941 gaaccagagg ctttggtcgc gctggcttcg ggcgcgcaac tgcaggcgca cggtttcggc
     9001 gagcagttcg aacaggaatt gcggtttggc cgcgtggctg aggcagagtt gctcccagtc
     9061 cacgccctgg cgggactggg ttttgcgcag gtgcagcagc aggcggtggc tcagctttgc
     9121 caggggcagc gccggctggg tattccagag ctcgaacacg ggcggcggcg gcgcggagcg
     9181 cagcaggtag aaggttctcc ccgccagcgc cagggttggg tggcggttga agaaccgcac
     9241 ttcgctcagc ggataaatcc ccccgccggg cacggtcagc cggtgggtaa aggccaggcc
     9301 cgctccgccg ttttccaggt gcggcgtcag ctcggcgacg gcgccctgga attcgagcgg
     9361 ctgcggcggt tccttgccga gagccagttc gagccgggcg gtgtggcccc agcgcgccag
     9421 ccagtgcaac agttcaaggt ctgacagcac caggggcgtt tcgccgttcc ggcgatggga
     9481 atgggtttct gccagccacc ggatgaattc ccagtcgccg ggcgggaaca gctcctgctc
     9541 gtgcgcggcg cgcgtggtca gttccaggat ttcgcccagc gtccggacct tctcgccggt
     9601 gcgcgggcgc gccaggcgca attccaccgc cagccggcag cacgcgggct gctccgggtc
     9661 gggttgcgga tggcagatcg cccgcacaaa ggcccgcggc gcgtcgaagt gcgccggggg
     9721 ctccgggaaa agccaggtct ccaggtggcg gaccaactgt tgctcccgcg ccgcctgctc
     9781 gaggcgctcg aaccggtcgc ggttggcgta gacactcaat tccgtgggca ccggccgccg
     9841 cgcgcgcaga aacaggaacg ccagctcctc caggcgcgcc gtcccttgca ccgccagcaa
     9901 gcgcggccac cagtcggagt tggggaaatc cttgcgcgac aggcggagct tttcgaagta
     9961 atcctcgagc aggagccacg cccgttgcga atccgcgcag ccggcgaaga cccgcagcaa
    10021 tttctcccgt tcggcgacca ccgcgcttga atcgcccagc tcgcgccgca ggtccgccag
    10081 cgcgccataa gtctttgtca gcgccgtgtt ttgcgcgtcg tacttcgata cctgtgacgc
    10141 gcgcgacccg tttccgtttc tcggcaaagc tttagccatc ttctgcactg acccttttcg
    10201 gggtctttca tctgcgcata gaaccagatg ggggtcaatc tcaaacgcca aaatttcggc
    10261 cggccgggcg cagttccgct gggaaagtag ccgcttgccg cgccccgcgc ctagcaaatg
    10321 cggggcaatt gctcgccgct ggacatgtcg aggatgcgcc gggcgccgat ggcgctttgc
    10381 aggagaacgc gggcggtgga ttggggggag gcctggccaa tccgggctgc cccggcgctg
    10441 acgggatgcg cgcgcaggat ttccagcgcg cgcgcggcgt cgcgttccgg cacaaaaacg
    10501 gcaaagcggc cttcgcaggc gacttgcagc gggtcgagcc cgagaatctc acaggcggcg
    10561 cggacgtctt cgcggacggg aatgagcttt tcctcgacct ggagggtgag gcgggcggct
    10621 tcggcgattt cattgagcac actggtgaga ccgccgcggg tgaggtcgcg caggcaatga
    10681 atttcgaggc cggcctgaat gagttgcagg acggcctcgg cgaccggggc ggagtcgctg
    10741 cggatggcgc tctcgaactc cagtccctcg cgcacggcca tgattgccat gccgtgccgg
    10801 ccgacgtcgc cgctgacgag gatggcgtcg ccgggctgga cgctctgggg cgcgatacgg
    10861 agcgaatgct ccaggatgcc aatcccggcg gtattgataa agaggccatc gcccttgcct
    10921 ttatccacaa ccttggtatc gccggtgatg atctggaccc cggcagcctg ggcggcctgc
    10981 tgcatggagg cggtgatttt ccacagggtc tccatcggca gcccctcttc gatgatgaaa
    11041 gcggcgctga ggtagcgcgg gcgcgcgccg gccatggcga ggtcgttcac cgtgccatgc
    11101 accgcgagcg agccgaggtc gccgccggga aagaaaaggg ggcgcacgac ataggaatcg
    11161 gtggtgaagg cgatgcgctg gccggcgagg tcgaacacgc tggcgtcatg ctgcgcttcg
    11221 agcagggagt tgcgaaaggc gggcacgaag agcttgccga tgagctggtg catgagcttg
    11281 ccgccgccgc catgcgccat cagcacatgc gggtaatcct ggatggggaa cggacaggag
    11341 agggtgaagt tggggggtgt ggtcatggtt cacggcggta gcggtagtag gcggcgcagg
    11401 cgccttcaga ggagaccatg gtcgcgccga ggggatgctc gggagtgcag cgggtgccga
    11461 aggcggggca ttcgcggggc ctgatcttgc cttgcagcac caggccgctg aggcattcgg
    11521 tgggctcatc cacgcggagg ccggccacgc cgaatttgtg ctcggcatcg aaggatgcgt
    11581 aggcgtcgcg caggcccagg ccgctgcggg ggatttcgcc gatgccgcgc cacttgcgcg
    11641 gggtgatctg gaacacctcg cggatgatct gctgcgcggg gcggttgcct tcctggcgca
    11701 cggcgcggct gtattggttc tcgacctcgg cccggccgct ctcgagttgc cgcacgcaca
    11761 tcagcacgcc ttgcagaatg tcgagcggct cgaagccggt cacgacgata ggcacgcggt
    11821 gctttgcggc gatgggtttg tattcctccc agcccatgac ggcgcagacg tggccggcgg
    11881 cgaggaagcc ctgcacgcgg cggtccggcg cgcccaggat ggcctcgatg gcgggcggca
    11941 ccagcacgtg cgagacgagc agggagaagt tcttgaggcc cttgcgggcc gcctggtaaa
    12001 ccgccatggc cgtggccggg gcggtggtct cgaagccgac ggcgaagaag acgacctctc
    12061 gggccgggtt ctgctcggcc agcttcacgg cgtccagcgg cgaataggtg atccgcacat
    12121 cgccgccggc ggctttgacg gccagcaggt cggtggtgct gccgggcacg cgcagcatat
    12181 cgccgaagga gcagaagatg acgccgggcc gggcggcgat ctcgagggct ttgtcaatca
    12241 gttcgagcga ggtgacgcag accgggcagc cggggccgtg gatgagcgtg acctggctgg
    12301 gcaggagctc gtctatgccg aacttgacga tggcatgggt ttggccgccg caaacctcca
    12361 tgagcgacca ggggcgcgtg gtgatgcgtg ccagctcgcg tgccagcttg tgcgcgcctt
    12421 gggcatcgcg gtattcgtcg aggtatttca ttgcggcggc ggacccggcg ccggggcggg
    12481 cgcgacgccc gcctcgcttt cctgcaactc gctcagctcc tgcatctggc gcagatactc
    12541 gaatacctgt ttggcctcct cctcatccac ccggctgagg gcgaagccga catggacgat
    12601 gacgtaatcg ccaagcttgg cctcgggtac gtaggcgagg ctgacttcct tgaggatgcc
    12661 gccgaagtcc actttgccgg tgcgcagcag cgggtcttcg ccgctgatgc tggtaatttt
    12721 acctggaata gccagacaca ttatccccca acctacaccg ggggcggcaa tccggcaatg
    12781 tcgcgcggag cggggcgctg aattcggccg ggtcaggacg gcagcaggcg ggcgttcggg
    12841 ctcagggcgg ccacgatctg gcccagcgcg aggccgccgt cgttgggcgg gacgcgctgg
    12901 tgccaatagg gacggaagcc cgcctcccgc aaacggcgca cggcgtgttc ggtcaggcag
    12961 cggttctgga aacagccgcc ggagagaacc acgcgcggct ggccgagccg ccgggcaacg
    13021 gcaatgatgg cctcgaccag tccctgatgg aagcgggcgg agatttggct cacgggaacg
    13081 ccgcggtcca ggtcggccaa tatagcttca agcatgggag cccagtcgag gatgagcggc
    13141 gcgcgcgagg aggagcgggc ctcgggttcc gagcggatgg gcagttcgta ggcttcgtcc
    13201 gcggaagcgg tgtcggggag ggcgaattcc agttccatgg ccgcctggcc ctcgtagtgc
    13261 gcccgttggc ggccgccggt gagcgccgcc accgcgtcga acaagcgccc gacgctcgag
    13321 gtcagcggcg agttgattcg acgggcgagc atggttttga gcggggccag ttcggcgggg
    13381 gtgaaagccc gcagcggggc caggtgggtc tgcgcaaagg cggcttcgcc gagcgcctca
    13441 tacagcaggc ccagggccgc gcgccgcggt tcccgagccg cggaatcgcc gccgggcagg
    13501 cggaaggggc ggagatgcgc ggcgcgcgcg caggcggttt ccgtgacgag gaaaaactcg
    13561 ccgccccaga cggtgccgtc gaggccgtag ccggtgccgt cccaggaaac cccgagcacc
    13621 ggcggcgcga gttcattttc cgccaggcag gaaaatacat gggcgaggtg atgctgaacg
    13681 gtgaggagtt gagggggcgg ggtttgggcc tcctcgccgt ggcggccccg ggcggcgagt
    13741 tcgtgcgcga actgggtgga aagataatcg gggtgggcgt cggcggcgat gatggatggg
    13801 cgcgcgtcgt aaagggtctg gaagtcggcg attacgcggc gaaaggccgc aaaggccagg
    13861 tcggtttcca ggtcgccgat atgctggccg atgaagacct gcggcccgac ggacagggcg
    13921 atggcgttct tgagatgcgc gccgaccgcc agcacggaat ggcccggagc cgatcgcgga
    13981 ccgcccaggg agaccgggag ttgaatgggc agcggggcat agccgcgggc gcggcgcaga
    14041 accagttcgc gccccgacat caggcgcacg atggaatcgt ccacatgccg gacgatgggg
    14101 cggttatgga ccagaaagag atcggcgatg ccgccaaggc ggtccagggc ctcgtattcg
    14161 tcggtgcaga tgggctcatc gctcaggttg ccgctggtgg ccacgacggg aaagccgagg
    14221 gcggcaagca gcagatgata ctgcggggcg taggggagca ttacgccgag attgggattg
    14281 tggggggcga gcgagggggc gaggggaagg gcggaaggcg cggggcgggg agcgcgggcg
    14341 cggcggagca ggacgatggg ggcttcggga gattgcagga gccgttcctc gagcggtgag
    14401 acctcgcact ccgcccggat ggccgccagc gacgggaaca taagcgcgaa aggcttttcc
    14461 tcacggtgct tcaatgcccg caggcggcaa acggcgtctt cattgtgcgc cgcgaccatc
    14521 agcagaaagc cgccgaggcc cttgacggcg acgattcggc cctggcgcaa ggcctccgcc
    14581 gccgcggcga tgggcgcgcc cgcgggcgcg gttgcggcgg gcagcggttt tccgctccgg
    14641 tcccacaatt cgagccgcgg gccgcaggcg gggcaggcgt tgggctgggc gtggaaccgg
    14701 cggtcgcgcg gatcgtcgta ttcggcctgg cattgcgggc acatgacgaa cgccttcatg
    14761 gaggtattcg ggcggtcgta cggcagcgat tcgaggatgc tgaaccgcgg gccgcaatgg
    14821 gtgcagttgg tgaaggggta gccatagcgg cggttgcgcg ggtcaaagat ttcagcccgg
    14881 caatcagcgc aggtggcaat gtccgggagc accagcgccg tccgggcgcc gccggggtcg
    14941 ctggggcgga tggcaaaccc ggcatagccg gccgggtcaa gccaggccgc ctcgagactt
    15001 tggattgaac tgcgcggcgg cttttccgcc gggagacgca accggaactt ctccaacgcg
    15061 gcgcgggggc cctcgacttc aatgaacacg ccctgggctg agttgctgac ccagccggcc
    15121 aggccgctct ccacggccag ccggtagatg aacggccgga agccgacgcc ctgcaccgcg
    15181 ccgcgcacga caatcctcag ccggacgctc gagcccatct cacgcatgat tccagttccc
    15241 gccgggcgcc gccgattccc cctgcccaac ccatgctttc tggcggttca tactcgcggg
    15301 cgttcaatcg tcgcggccgg cttcgtgaac gcggatgaag cgttggaaca cgaacaggcc
    15361 cacgggagca atcagggtgc tcaagccaat gatgagccag agcgttcccg agtggcgggg
    15421 gccggtggcc gggcagaacc ggtcgagcaa tacgccggag agcggcccca ccccgatttt
    15481 cgccaggaaa aacggcagcg aggacatggc catgtaggag gcttcctgac ccttgggcgc
    15541 gatggccgcg gtgtattcat aaagccgcgg cgagtagaag gactcgccga tggagaggaa
    15601 caggacaaag aggaaaatca tcacgtagta ggggttgacc gtgccgctca ggcccagcca
    15661 aacatgggcg atgccgtggc cgaaccagcc gtcggccagc ggccggaacc attggggcgg
    15721 gacggccatg atgaaaaccg acgccgccgc aatgaagctg ccgaaaataa ccatgcgata
    15781 ggccgagatc cgctgcgtga gcacccccac caacggcgcc agaaacacaa tcaggatatt
    15841 gttcagcccg tagagatgcc cgacgggcgc gccctcgccc agttcgcgaa tgccgaaggt
    15901 cgggtaggtg taatccatat gcacgaaaat catcttgata aacacggcca gcgacagaaa
    15961 ggccaggaac ttgtaaaagc cggtctgccg ccacaggccg gcgaagatgc gggccgtatc
    16021 gcgcgcggta tcgccgatcg tcaaccggag cacccgcaag gcattctggc cgggatattt
    16081 cacctgctcc ggcgtgatga caaccccttg atccgtggct tcgacccctt cacggagcca
    16141 gaagtagatg atcaggaagc tggggatgga cagcgccagg ctggccagca gaatggcgcg
    16201 gtaggtgctg agctgcatgc cgagcccggg caaagtgaag ccgtcatgct cccccagctt
    16261 cgcgcgcagg gcatcgaaaa tcaggacgcc gatgaggaat cccgcgttca taatggcgta
    16321 gaagatggag aaggcgatgg aacgttgcgc cgtggtggtg tagcgccgca gggcggcgat
    16381 ggtcaccggc gcgcccatgc cctccccgag cgccagcacg gtcaggccga cccccagcgc
    16441 cagccacttg accgtggtaa acgtgatgaa gacgcgcgag atgatgcaaa gcgcgaaacc
    16501 gagcaggaac gtcttgcgca accccagcgc gtcggtcagc gatccgacca gcacgatgca
    16561 gagcgtcagc cccgccgacc acagcagcac caccaggccc gactggacgt cgttgtagcc
    16621 caggtccgag gagagccaaa gccgcaaggt gaagttcacc acccggtagg ccaggttgct
    16681 gaacagcacg atggcaaaca cgatccataa ctcgcgcacc gcgccaaaga gcacggtgaa
    16741 cttgcccaga aagctccgca gcgggccggg ggacggcggg ttgttcgggt caggcatggg
    16801 gaagcggggg gacgcgccgg ctcagggcgg ggatgcgggc gcggaaagag gattttcccg
    16861 ccggcatttt agtatggact gcaggcatga cgcagtgtag tcttgtggcc cgaattgcag
    16921 caagcgtatg actggaattc tttacggccc gcaatccggc ggcttccgga agcagccggt
    16981 ggcctcaccg cccggctttc gccgggaggg ggggcgggcg tttacgctga tcgagctgct
    17041 ggtcgttctg gcaatcattg gcctcctggc cagcctgctc ctgccggcat tggcccgggc
    17101 ccgggaggcg tccctgcgcg tcaaatgcgc caatcatctc aaacaactgg gatatgccat
    17161 ccggatgtat gccgacgaca gcaacggccg ttaccccatg cgcgcgctgg ccggctactg
    17221 gcccgcggca ttgctcgaat actaccagtc caccaacatc ctgctctgcc tgtccgaccg
    17281 gcgccgcggg ccgccgcaga cggattttgc ggcgcagttg gcggcggacc gggccaaccg
    17341 cagctacctc atcaatgggt ggaatgatta cttcggcacc gcgttaaatg ccgccagcgc
    17401 catgcgcgag gccgccgtga tttaccccgc ggaaacgatt gtcttcggcg aaaagaagaa
    17461 ttccgtggcg cactacttca tggacctttg cgaagaccac ggcaatgacc tcgactccgt
    17521 cgagcaggcc tgccactccg ccagccaaaa gagcgtctat tccgggggcg ccaacttcac
    17581 ttacgcggat ggcagcgtgc gctatatgaa gtatggaacg acggtctggc ccctgaacct
    17641 ctgggccgta agcaccacca accgccttct cttcgcctgg caacggtagc gcccgcgctg
    17701 cccggcaggg tcagtggaag cgcgccgcgg cagaccccat accctcccgc ggctttccac
    17761 ccccggcaac ccaccagccc ccggcactat cccgatccct gcaggccgcg tgcccacacg
    17821 cggcgctccg caccggcctg ccaccaccac cccccaaaaa ttgcccttgc gcgaagcctc
    17881 cctgcccgcc agccttcctc ccgacccttg gggtgggggc ggctggaact cccagcccag
    17941 ggaacggcag gaagcggagt cgctctccgg cccgtaatag acaaacaatt aaaataacca
    18001 ttagagagga cagacattat gaaaatacta tctgctccgc gcacgggcag tctgggcgcg
    18061 gaaacatact accaatcccc cttcggcgt
//
LOCUS       DAOPQG010000076        17668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3824_length_17668_cov_14.546982, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000076 DAOPQG010000000
VERSION     DAOPQG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 17668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17668
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_17668_cov_14.546982"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..2781
                     /locus_tag="PKI07_09520"
     CDS             217..2781
                     /locus_tag="PKI07_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOC51329.1"
                     /translation="MRIISSPSLPLIRLSWLLAACAFGFPAWSASTGERFAFVIPGDD
                     ASRSATDFSGLSPKAAGADGFVRIQDGHFYTGPKRLKIWGVNLCFGGNFPAQEDAAKA
                     AAHLAKLGVNGVRMHHHDTAAAPSGIWGPVVGGRRTLDPDMLDRQDYFLDQLHRHGIY
                     ANLNLHVGRTFTAAEGFVASNLPDAVRYNKYLIYYEPRMRALFKEFCRDYLTHTNRYR
                     RLPRTADPGIALIELSNENSFSKLGPGLAARLPEPYRGEFKRQWNRWLGSRHASTEAL
                     KRAWGATVEPPGEYVLAAAEWRENLGGWWLHQSPEFPVKAVLDQPGPEPGLPALRLEI
                     PKAAPELHRQELQFPGLRLEPSRTYTLSFWIKAEAVRSLYVDVSRQGPHDWNPVGLAE
                     TVPAGPAWTQVKHVFSSAAEVPDKVRICFKFGRDATAFSLAAVSLQRGGRFIVVPDGQ
                     SLEQGNVEIPQSGWCEAARREAQQFMVDTEKGFIREVADFLKRDLGVRVPITASQITY
                     HGAEIVAATCDYADIHAYWEHPHFPGRPWDPADWTIRNTPMELAPGTDSLLTRAPWRL
                     LDRPFTLSEWNIPDPNDYAASAVPFAAMVAALQDWDGVFFFDYHSRATEWEAERMQGY
                     FSFNGQPVKLALLSACANLYRRGDLQPLPAVAAGTLRQMAPAALALSQRIGIDPAADS
                     PALANAPAGQLLASPDGRVVWDARDRNLAHVQINTPNSRAVWGLIAGQTFDLGGVQLT
                     LGPADRNYAALVLTSLDGKPLEKARRMLLTAVGSAENQGMVWNDARTSVGNRWGSGPA
                     RVNGIPVEVSLPFRVKQVRVLDSRGQPQTAVPVRVDGRISRFAIGPEHRTLWYEIR"
     gene            complement(2800..4086)
                     /locus_tag="PKI07_09525"
     CDS             complement(2800..4086)
                     /locus_tag="PKI07_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOC51330.1"
                     /translation="MKTAVVGLGYVGLPLSLQFARSGVTVLGLDVDPAKVEALLAGQS
                     YIKHISSAEVAALVKSGHLSASTDFGRIREVEAVIICVPTPLNKNREPDLSFILETGR
                     SIARHLQKGMLVVLESTTYPGTTDEDLRAVLENGSGLKAGQDFHLAFSPEREDPGNPA
                     SQVALIPKVVGGYSPACLKKAVALYSRAVKTVVPVSSCRAAEAAKLLENTFRGVNIAL
                     VNELKIVYNAMGIDIWEVIHAAKTKPFGFMPFYPGPGLGGHCIPIDPFYLTWKAREFG
                     QNTRFIELAGEINTSMPLYVVHQLADALNARQKSINGSSVLVLGLAYKPDVDDERESP
                     SYVLMEMLKTRGARVAYYDPYVPVIKPTREHAHWAGTQSVAWNQETIKAFDVVLIATN
                     HSCVNYRELAEWAECIVDTRNAMGGVPTLPNQVWKA"
     gene            complement(4122..4616)
                     /locus_tag="PKI07_09530"
     CDS             complement(4122..4616)
                     /locus_tag="PKI07_09530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOC51331.1"
                     /translation="MSGSEQLYQRIAADVKLGQRVKIFAFTNLYGCEIGDDTKIGTFV
                     EIQKGARIGQRCKISSHSFICEGVTIEDEVFIGHGVTFINDRFPRATNTDGSLQTEAD
                     WKCVPTTVRQGASIGSGATILCGVEIGRRATVGAGSVVTRNVPDGAVVAGNPARLLRA
                     GAAA"
     gene            complement(4613..5731)
                     /locus_tag="PKI07_09535"
     CDS             complement(4613..5731)
                     /locus_tag="PKI07_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51332.1"
                     /translation="MKQTIKVGVAGCGYWGPNLIRNLRQSPDCQLKLLCDPSEQRLAH
                     MRRLYPEVATTRQFDDLLNGADLDALVIATPVRYHYEMAMAGLRAGKHVFVEKPMART
                     EAEAEEMAALAEREGLVLMVGHTFLFSPAVRRMKEIVDAGDIGEVQYIAARRLNLGLF
                     QKDINVAWDLAPHDISILLHLLEEHPVSVSCQGSCHVTRSIEDVTMMYLTFARNRCAM
                     IHNSWLDPKKVRQMTVVGSQRMIVYDDTEPLEKLKIYDARVEVPPHYDTFAEFTYSYH
                     YGDAYVPYIKQDEPLKLECQHFLECIRAKCTPIAGGRLGLEVVRILEAASESLRQQGA
                     PVSLEAVTQWNNGHDGNGKGSESSGRVAASPARRILAA"
     gene            complement(5759..6445)
                     /locus_tag="PKI07_09540"
     CDS             complement(5759..6445)
                     /locus_tag="PKI07_09540"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOC51333.1"
                     /translation="MAVRHGAKGLPMWKRALDLALVVMLAPGLLVIGGVVALVVACGS
                     RGSVFFRQRRVGYQGQIFECYKFRTMQENADTQQHRAHVQQLMEADVPMTKLDAKADP
                     RLVPLGRLLRATGLDELPQVLNIIRGEMSFVGPRPCIPYEFEHYQSWQRRRFAAAPGL
                     TGLWQVSGKNRTTFNEMIRLDIEYSERQSLGLDLWIIMKTFPALLQQCVDLRAGKRRQ
                     PSAAVAVSVG"
     gene            complement(7105..7872)
                     /locus_tag="PKI07_09545"
     CDS             complement(7105..7872)
                     /locus_tag="PKI07_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOC51334.1"
                     /translation="MSIAYVLLAYVLAVGGGALGILLGNSHERLCALISLGAGTLLGV
                     TLLAILPESFATLRWRGVLLAVGSGYAVFAVISKYIFHICPACAASHFDEAATHRFGE
                     IATAMMVALAIHCLADGVALAAGHEAEASHAHSSRVLDLSLIVAICVHKVPEGLALGG
                     LLLGAGFSRMQTLLRVAAIEATTLVGGVLGWFFLREASLFWLDAAVAHVGGGFLYLAV
                     HAVLGEILKHHKALVLTCFALGFGIIAALVCLLRLLH"
     gene            complement(8284..9351)
                     /locus_tag="PKI07_09550"
     CDS             complement(8284..9351)
                     /locus_tag="PKI07_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOC51335.1"
                     /translation="MRFISGVIEKLQRHPKRIVFPEGTEPRVLLAARQFYALRLGVPI
                     LLGDPVRIQEVARDLKVALNGIRLLNPADSEELDNYASRFRRLHQYRGKRMKMLEARD
                     AVLQPNYFGAMMLAMHAADGFVAGVRQVTAGVLRPLFQTIKPAPHSKTVASCLIMEVE
                     DSRLGEQGVLFLADCDVIEDPSVEQLADIAVSTAQLARHLLGARPRVALLSFSTKGSA
                     AHPSVGKVQAATARAQQKASAQKLDADFDGELQVDTSLVPEIAARKLPDSKLGGHANV
                     LIFPDLNSAQIASKLARLLARANAYGHILLGLDRPAADVSRGSTTRDLLGVAAIIGVQ
                     SIDYQALYPGAGQRLPGDRVR"
     gene            9476..10204
                     /locus_tag="PKI07_09555"
     CDS             9476..10204
                     /locus_tag="PKI07_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOC51336.1"
                     /translation="MLETIERLLILQDCDRKIRREQGELARIEPERQTFNAKAAAAQS
                     ALENARQRLKQIESARKDLELEAQVKKQMIDRYAHQQLLTRKNDEYRALASQIETCKS
                     AIFELENQQIELMEQAEAVQKEIAHATATASDTRKLVEDQLAQLAAREANLRRELDEL
                     RARRETLAGNVEQTARSRYERLLRSKGENVLVGVQHGVCGGCHMKVPPQLLVTCQAEK
                     ELVACSNCGRMLYYTPDMDLAVTE"
     gene            10332..10788
                     /gene="rnpB"
                     /locus_tag="PKI07_09560"
     ncRNA           10332..10788
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI07_09560"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            10819..11796
                     /locus_tag="PKI07_09565"
     CDS             10819..11796
                     /locus_tag="PKI07_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51337.1"
                     /translation="MRNACLLRVILLAGCLANDPCPLSALPLQRADVPAAPAWVLHVD
                     CDKLRPSAMGQYLLEGMEKPEIRARLGAFWAALQLDPRKHLRGLTLYGRADAPADGTL
                     LVYGDFQAGQLERAARAAKDHLTTPYQKHRIHQWLDTSGNRNPSADQRVYGAMHHQRV
                     IILSRQETNVAHALDVLDCTVRSLRESGQCAQLGNQAGSVFLQAAATKVEPLPVHPAT
                     IILRLARAARLEMSEASDRFTATLSLEADGNATATQILSAGQALLKLVKLQPNAASAM
                     RLISAINLKQQGSEVEATLAASASEIIALLKNEGSQPESKQPKPSSPNN"
     gene            complement(11900..13003)
                     /locus_tag="PKI07_09570"
     CDS             complement(11900..13003)
                     /locus_tag="PKI07_09570"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOC51338.1"
                     /translation="MSSNAQCPERLIFAATPRWRPGALLVLFLGGLMGGHPLPAQLLA
                     PASPGSTAPQADIFVVQDDTATAAFAPRWDRVRLMVNCAITNLTHCATMPEAWRSLIA
                     TQDVVGIKVFSAPGPNSGTRPAVVAAVIEGLLAAGLPPKQIVVWDRRLTDLRLAGYGT
                     LAQKYGIRLAGSIEAGWDLEVFYDPDSPILGNLVWGDLEFGRKGESVGRKSFVSQLLT
                     RQVNKLINVTPLLNHNAAGVAGNLYSLATGSVDNVIRFESQPESLATAVPEIYGLPVL
                     SDHVVLNITDALICQYEGGERGLLHYSTVLNQLRFSRDPVALDVLSLQELDRQRQFAD
                     VPAVKRNLRLYNNAALLQLGVSDLRRIRVERIP"
     gene            13034..14896
                     /locus_tag="PKI07_09575"
     CDS             13034..14896
                     /locus_tag="PKI07_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51339.1"
                     /translation="MLTTKNAAARDPGARMAVSGPRRCWRAGLAGLLALLAGCTPAGP
                     HAFLEGRRLMNRGEYQQAIEKLRAATVLLGGTNALAWNDLGLAYHHAGEAVEAEHAYR
                     RALTLNQDLSEARYNLGCLWLSQNRLEAAKTEFAAYTLRRPNEAPGFLKLGTAQLRAR
                     ELNAAEKSFREALRLQRQNPEALNGLGLTLLGRGQATAAAHCFEATLKQQADYGPALL
                     NLAIVSHQHLRNRQVALEKYRAYATLKPVPANAQAVQATIRQLEMELASPARPATTGP
                     TVPSIQVTPPPQPVAPPVTSTVATHKPEPILEPPKTTTASAAKASPTSVPSPAPIIPP
                     PPPATTGIVRPSVESALQPSPPVVARPIPAQGSPTEPLIITSSVPARVTASQTDKRGF
                     FQRINPLNLVRSKEKTPPQPTPLGPTPGTELPESAGTVTTGAAPTPGVFTAPEPGNQL
                     SRYAYHSPAVPSRGNRPEAEAAFTRGVRAQQTRRWTEAKQAYQQAVQLDPSLFEAHYN
                     LGLVAAETGNLPLALRAYETALALEPSSNDARYNFALVLKRANCPRDAVNELEKIVAH
                     SPNETRAHLALGNLYAQQLKQPTRARQHYLKVIELEPQHPQASAINFWLASPQP"
     gene            14911..15501
                     /locus_tag="PKI07_09580"
     CDS             14911..15501
                     /locus_tag="PKI07_09580"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOC51340.1"
                     /translation="MPTSRRSWPRLILASASPRRAELLRSLGLAFEVQPADASETHHA
                     QLSACELCRINALRKARWVARKVPHALVLAADTLVSLEETLLGKPATQAAARKMLRQL
                     QGRTHLVVTGVCLLHWRTQCQVLFTESTAVAFRRLTDYQIRQYLSRVNPLDKAGAYAI
                     QEHGDLIIEEIAGSYTNVVGLPLERLRMELAAWLGT"
     gene            15702..17189
                     /locus_tag="PKI07_09585"
     CDS             15702..17189
                     /locus_tag="PKI07_09585"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOC51341.1"
                     /translation="MKLSALLSVLPEKKVFGNQAPEITGVVYDPLRVTPGCLYVAINI
                     YTQLDKIEIPDGHPLLPDAVRAGAVAVVLQQDQPVPAGVVKILVPDSRYALALLAVEF
                     YRHPARSLQLIGVTGTNGKTTTTHVIESLLMTRYRAGLIGTLYYKINGRICNSKDTTP
                     EPPDLAAIFTEMVNEKCDFCTMEVSSHAVDFHRVVGVDYQAAAWTNLSQDHLDWHKTM
                     ENYRHAKLRWLSSLGPQQHVALNHDDPSAPFFIERVRAQKVLYGLKNKADVTARDYRF
                     SADGTRFTLVTPRGEIEVQAKLRGEFNLYNMLAGVAAVLHTPLTLAELKQGLEKNIAV
                     AGRFQPIALGQPFSVIIDYAHTPDGMETVLRAARAMNPQRLITVFGCGGDRDRAKRPL
                     MAAAVEAHSDRFIITDDNPRTEDPDQIVGDIMAGLQKKQSGAHEVIHNRRDAIRHALD
                     QAQPGDLVLVAGKGHESSQILKDRTIHFNDYEVAEELLREKGYGG"
BASE COUNT         3583 a         5794 c         5084 g         3207 t
ORIGIN      
        1 tttgggcggc ttggggagga ggaaggctca tgtcctctgt tcccgggcga cctatgaggc
       61 tccattcctg gggctggcaa tacagccgaa tcgctcggac ttgcctggct ggagtggtcc
      121 tcgggtgccc ttgtcacaag ttcatcctgg gcaatttcgg cgtgcctggg caggaatctg
      181 gcgctttggg cccggatatg atagaacccc tggcgtatga gaataatctc cagccccagt
      241 ttgcccctca tccgtctcag ttggttgctg gccgcatgcg cgttcgggtt cccggcttgg
      301 agcgcctcaa ccggggagcg gtttgctttt gttattccgg gcgacgatgc cagcaggtca
      361 gctaccgatt tttcggggct ttcgcccaag gccgcgggag ccgacggctt tgtgcgtatt
      421 caggacggtc acttctacac cgggcctaaa cgcctgaaaa tctggggggt gaacctgtgc
      481 ttcggcggca attttcccgc gcaggaggat gccgcaaaag cggccgcgca cctggccaag
      541 ctgggcgtca acggtgtgcg catgcaccac cacgacaccg ccgctgcgcc cagcgggatt
      601 tggggcccgg tcgtcggcgg gcggcgcacg ctggatccgg acatgctgga tcggcaggat
      661 tatttcctgg atcagcttca tcggcatggg atctatgcca acctgaacct tcacgtcggg
      721 cgcactttca cggccgccga aggtttcgtc gcctcgaacc ttcccgacgc ggttcgttat
      781 aacaagtacc tgatctatta cgagccccgc atgagggctt tattcaagga gttttgccgc
      841 gattacctga cgcacaccaa ccgctaccgt cgcctgccgc gcacagccga ccccggcatc
      901 gccctgattg aattgagcaa tgaaaactcc tttagcaagc tcgggccggg cctggccgca
      961 cggctgccgg agccgtatcg aggcgagttc aaacggcagt ggaaccgctg gttgggctcc
     1021 cggcacgctt cgacggaagc cttgaaacgg gcctggggcg cgacggtcga gccgcccgga
     1081 gagtatgtgc tcgccgccgc ggaatggcgg gaaaaccttg gcggctggtg gctgcaccaa
     1141 agcccggagt tccccgtcaa ggccgtgctc gatcagcccg ggccggagcc tggcctcccg
     1201 gcgctgcgcc tggagattcc gaaagccgcc ccggagttgc accggcagga acttcaattc
     1261 ccgggcctgc gtcttgaacc ttcccgcacc tatacccttt cgttctggat caaagctgaa
     1321 gccgtccgct cgctctatgt ggatgtatcc cggcaagggc cgcacgattg gaacccggtg
     1381 gggctggcgg agacggtgcc agccgggccg gcctggacgc aagtaaagca tgtgttttcc
     1441 agcgccgccg aggtcccgga caaagttcgc atctgtttca agttcggccg ggatgccacg
     1501 gcgttctcgc tggccgccgt gagcttgcag cgcggcggca gattcatcgt cgtgcccgac
     1561 ggacaaagcc tcgaacaggg caacgtggaa atcccgcagt ccggctggtg cgaagcggca
     1621 cggcgggagg cgcagcagtt catggtggac acggaaaaag gcttcattcg cgaggtggcg
     1681 gactttctca agcgggatct cggcgtgcgc gtccccatca cggcttccca gatcacgtat
     1741 cacggggcgg aaattgtggc cgcgacctgc gattacgccg atatccatgc ttactgggaa
     1801 cacccgcact ttcccggccg cccctgggac ccggccgact ggacgatccg caataccccg
     1861 atggaactgg cgccgggaac tgacagcctg ctgacccgcg cgccgtggcg gctgttggat
     1921 cggcccttca cgctctcgga atggaatatc ccggacccga atgattacgc tgccagcgcg
     1981 gtgccgtttg cggcgatggt ggcggccttg caggattggg acggggtttt cttctttgat
     2041 tatcacagcc gcgcaaccga atgggaggcc gagcggatgc aggggtattt ttctttcaac
     2101 ggccagccgg tgaaactggc cctgctcagc gcgtgcgcca acctctaccg gcgcggcgat
     2161 ctccagccgc tgcccgcggt ggccgccggc acgctgcgcc agatggcgcc ggcggcgctt
     2221 gccctgagcc agcgcattgg cattgatccc gcggcggact cccctgccct ggcaaacgcg
     2281 ccggccgggc aactgctcgc aagccccgac gggcgggtcg tatgggacgc ccgcgaccgc
     2341 aacctggctc acgtgcaaat caacacaccc aactcgcgcg cggtgtgggg cctgattgcc
     2401 gggcaaacgt tcgacctggg cggagtccaa ctcacactgg gcccggccga tcgcaactat
     2461 gcggcgctgg ttttgaccag cctcgacgga aaaccgctgg agaaagcgcg ccggatgctg
     2521 ctgacggcgg tcgggtccgc ggaaaatcag ggcatggtct ggaacgatgc gcgcacgtcg
     2581 gtcggcaacc gatggggctc cggcccggcg cgggtcaatg gcatcccggt ggaggtcagc
     2641 ctgccgtttc gcgtcaagca agtgcgggtg ctggacagcc gcggccagcc ccagaccgcc
     2701 gttcccgttc gcgttgatgg ccgcatctca cgcttcgcca tcggcccgga gcaccgcacg
     2761 ctctggtacg aaatccggtg accgcgccgc cgcggccgct caggccttcc acacttgatt
     2821 gggaagcgtg ggaacgccgc ccatcgcatt ccgcgtgtcc acaatgcact ccgcccactc
     2881 cgccaactcc cggtagttga cgcaagagtg gtttgtcgcg atcagcacca catcgaaggc
     2941 cttgatggtt tcctggttcc aggccaccga ctgggtgccc gcccaatggg catgctcgcg
     3001 ggtgggtttg atgaccggga cgtacggatc gtagtaggcc acccgggcgc cgcgcgtctt
     3061 gagcatttcc atcagcacat agctggggga ctcgcgctca tcgtccacat ccggcttgta
     3121 ggccagcccc aacacgagca ccgagctgcc gttgatggac ttttggcggg cgttcagcgc
     3181 gtcggccaat tggtgcacca catacagcgg catggaggtg ttgatctccc cggccaattc
     3241 gatgaagcgg gtattctggc caaactcgcg ggccttccag gtcaggtaga aggggtcaat
     3301 cgggatgcag tgtcccccca ggcccggccc cggataaaac ggcataaacc cgaacggttt
     3361 ggttttcgcc gcgtggatga cttcccagat gtctatgccc atggcgttgt aaacgatctt
     3421 aagctcgttg acgagggcaa tattgacgcc gcgaaaagtg ttctcaagca gcttggccgc
     3481 ctctgccgcg cgacaggacg acaccgggac aacggtcttg acggcccgcg aatacagggc
     3541 caccgccttt ttaaggcagg ccggcgagta gccgcccacc accttcggaa tcaacgcgac
     3601 ctggctggcg ggattgccgg ggtcctcgcg ttccggcgaa aaggccagat gaaaatcctg
     3661 ccctgctttc aggccggacc cattctcaag caccgcgcgc agatcctcgt cggtcgtgcc
     3721 gggataggtg gtggactcca acaccaccag catccctttt tgcaaatgcc gcgcgatgct
     3781 ccgcccggtt tcgaggatga acgagaggtc cggctcccga ttcttgttca gcggcgtggg
     3841 cacgcaaatg ataacggctt ccacttcacg gatgcggccg aaatcggtcg aggccgacag
     3901 atggcctgac ttcaccagag cggcgacctc cgcggacgaa atgtgcttga tatagctttg
     3961 ccccgccagc aaagcctcca ctttcgcggg gtccacgtcc aggcccagca ccgtcacccc
     4021 ggaacgcgcg aactggagcg acaggggcaa ccccacataa cctaatccca ctacggctgt
     4081 cttcatgcta tgattgctct tgccgggttt cgccccgggc ctcacgccgc cgccccggcg
     4141 cgcaacaacc gcgccggatt gcccgcgacc acggcgccgt ccggaacatt ccgcgtgacc
     4201 acgctgcccg cgccgaccgt cgcccgccgt ccaatttcca ctccgcacag aatggttgcg
     4261 cccgaaccga tcgaggcgcc ctgccggact gtcgtgggga cgcatttcca gtccgcttcg
     4321 gtctggaggc tgccgtccgt attggtcgcc cgcggaaagc ggtcgttgat gaaagtcacg
     4381 ccgtggccga tgaagacttc atcttcaatc gtcacgccct cgcaaataaa gctgtggctg
     4441 gaaatcttgc accgctggcc gatgcgcgcg cctttttgga tttcaacaaa cgtcccgatt
     4501 ttggtatcgt ccccaatctc gcagccgtag aggttggtaa aggcgaagat cttgacccgc
     4561 tggccaagct tcacatccgc ggctatacgc tggtagagct gctcgctgcc gctcatgcag
     4621 ccagtatgcg gcgagccggg ctggcggcca ctcggccaga tgactcgctg cctttgccgt
     4681 tgccgtcgtg gccgttgttc cactgggtca ccgcttccaa cgacaccggg gccccctgct
     4741 ggcgcagcga ttcgctcgcc gcttccagaa tccgcaccac ctccagcccc aggcggcccc
     4801 cggcgatcgg cgtgcacttg gcgcgaatgc attccaggaa atgctggcat tccagcttca
     4861 atggctcgtc ctgcttgata tacggcacgt aggcgtcgcc ataatgatag gaataggtga
     4921 actccgcaaa ggtgtcgtaa tgcggcggca cttccacccg cgcatcgtaa atcttcagct
     4981 tttccagcgg ctcggtgtcg tcatatacaa tcatccgctg cgagcccacc acggtcattt
     5041 gccgcacctt cttgggatcc agccagctat tgtggatcat ggcgcaacgg ttccgggcga
     5101 acgtgaggta catcatggtc acgtcctcga tgctgcgcgt cacatggcag cttccctggc
     5161 aggaaacgct gaccggatgc tcttccagca aatgcagcag gatcgaaatg tcatgcggcg
     5221 ccaggtccca cgccacgttg atgtctttct ggaacagtcc caagttcaac cgccgcgccg
     5281 cgatgtattg cacttccccg atatcgcccg cgtccacgat ctccttcatc cggcgcaccg
     5341 ccggtgaaaa caggaaggta tgccccacca tcagcaccag cccctcgcgc tcggccaggg
     5401 ccgccatttc ctcggcttcc gcctcggtgc gcgccatggg cttctcgacg aatacgtgct
     5461 tgccggctcg cagcccggcc atcgccatct cgtaatgata gcgcaccggg gtggcaatca
     5521 ccaaggcgtc caggtccgcg ccgttcagca aatcatcaaa ctggcgtgtg gtggccactt
     5581 ccggatacag ccgccgcatg tgcgccagcc gctgctcgct cgggtcgcat agcagcttga
     5641 gctggcagtc cggcgattgc cgcaggtttc ggatcaggtt cgggccccag tagccgcaac
     5701 ccgccactcc caccttgatg gtttgcttca taattcttgt tcgttgttct tcggtcgttt
     5761 atcccacgct gaccgccact gccgcggacg gctgccggcg ctttcccgcc cgcagatcca
     5821 cgcactgttg cagcagcgcc gggaaggttt tcataatgat ccacaagtcc agccccaggc
     5881 tctggcgctc cgaatattca atgtccagcc ggatcatctc attaaaagtc gtgcggttct
     5941 ttccgctcac ctgccacaat cccgtcagcc ccggggccgc ggcaaaccgc cgccgctgcc
     6001 atgactggta gtgctcgaat tcgtaaggaa tgcaagggcg cggccccacg aaactcattt
     6061 cgccgcgaat gatgttcaaa acctgcggca actcgtccaa ccccgtcgcc cgcagcaacc
     6121 ggcccaatgg caccagccgg ggatccgctt tggcatccag tttcgtcatc ggcacgtccg
     6181 cctccatcaa ctgctgcaca tgcgcccgat gctgctgcgt gtcggcattc tcctgcattg
     6241 tccgaaactt gtagcactcg aaaatctgcc cctggtagcc cacccgccgt tgccggaaaa
     6301 acaccgaccc ccgcgacccg cacgccacca ccagagccac caccccgccg atgaccaaca
     6361 atcccggcgc cagcatcacc accagcgcca ggtctagcgc ccgcttccac atcggcaacc
     6421 ctttcgcccc atgccgaacc gccacacttt gtccacttgc cgatccacct agaatctttc
     6481 cagccgaacc agccgaaatc tctgagctta tcatcatgct cagacatctt cccccatctt
     6541 ccaaaatcgg caactccgct cattacggag tggctttccg tgaaactacc gacagcccgg
     6601 cttccggttt ccattcccgc cgcgcctgcc ctgccctgtg ctgtggcggt ttctcgcatg
     6661 gcgggctccc tcgtgcgttc ccccgggcgc ctaagccgcc cgctttcctg ccgcatccaa
     6721 ccccggcaat acgggcaatc cttcaacgcc cgtctcctcc gtcagttcgt taaccgacgg
     6781 ccactgtccg ggtcggcgtg gggttgctcg cgccgccgcc gcagccaggg caaaacccgt
     6841 cagcgtcgaa acacaaaacc acgaaataag cgccatagcc atgcccccat tccagcaagc
     6901 agcgtgccct gtcacgcttg ccgctgaaat cccgggttta gggaaaaagc tccccgcgct
     6961 ctatctcata tttggacact tagccgcccg gcgcgaaacc ggctgtccca ttatgagaca
     7021 ctccgcccgg aagccaattc cctccctgcc tcccccgcct cgctgcaacc ctttccacgc
     7081 gatttctgct tttcccggca gggttcagtg caacagccga agcaaacaaa ccaacgcggc
     7141 gataatacca aagcccaacg caaaacaggt tagcacaagc gctttgtggt gtttgaggat
     7201 ttcgcccaaa accgcatgca ccgccaaata gagaaacccg ccgccgacat gggcgaccgc
     7261 ggcatccagc cagaacaacg aagcttcgcg taaaaagaac caacccagca cgcctcccac
     7321 caaggtggtc gcctcgatcg ccgcaacccg cagcaacgtc tgcatccggc taaagccggc
     7381 gcccaacaat aacccgccca gcgccaatcc ttccgggact ttatgcacgc aaatggcaac
     7441 gatcagggac aagtccagca cgcggctgct gtgcgcgtgg gacgcctcgg cttcgtgccc
     7501 cgcagccagc gccacaccgt cggccaggca atgaatcgcc agcgccacca tcatcgcggt
     7561 ggcaatctcg ccgaaacgat gtgtggcggc ttcgtcgaaa tggctggccg cgcaggcagg
     7621 gcagatatga aagatgtatt tgctgatcac ggcaaacacc gcatagcccg accccaccgc
     7681 cagcaacact ccccgccagc gcagggtcgc aaagctctca ggcaaaatcg ccaagagcgt
     7741 cacccccagc aatgtgccgg cgcccaggct gatcagcgca cacaatcgct catgcgaatt
     7801 gcccagcagt atccccaacg cgccgccccc cacggccagg acatacgcca gcaacacata
     7861 ggctatggac atggtttcca tgatcgtttc cgcttagaat gccgcgccac ctccccaaac
     7921 tcaagccggc agcaaatcgg ttaagacgct cccggttagc gcgcgcatgg cgaaactccc
     7981 gggaggtgcg cctgcttttc tttccgccaa ccgccgccgc ttcgaacggc ggcaatatcc
     8041 gcgcgccatc ccgcttgcgc cctcccccgg cttgtcccca gaactggtga ctagctgcaa
     8101 attgggatcc tggtaagttt ggggtgtgtt aacgatgctg gtgtgtggca tcggtgaaga
     8161 tttaatcggt tctcaatgta gaaatgtggc agaaagaccg ggtgaataac ccggcctttt
     8221 agtgtacccg tagaatctcg caacttctgg caatgaggct ccgtcccttt tccgatccct
     8281 ttgtcagcga acgcgatctc cgggtaatcg ctggccggca ccagggtaca gagcctggta
     8341 atcaatcgat tggacgccaa tgatggcggc cacaccgaga aggtcgcggg tggtggaccc
     8401 gcgggaaaca tcagccgccg ggcggtccag gcccagcagg atgtggccgt aagcgttggc
     8461 gcgagccagg agacgggcca gtttactggc gatctgggcg gagttgagat caggaaagat
     8521 gagaacgttg gcatggccgc cgagtttgct gtcgggcagt ttgcgggcgg cgatttccgg
     8581 cactagcgac gtatccacct gaagctcgcc atcaaagtcg gcatcgagct tttgggcgga
     8641 ggccttttgc tgggcacgtg cggtggccgc ctggactttg cccacggagg ggtgagccgc
     8701 actgcctttg gtggaaaatg aaagcaaagc cacccgggga cgcgccccga gcaaatgccg
     8761 ggcgagttgg gccgtggaaa ccgcgatgtc cgccaattgc tcaaccgagg gatcttcgat
     8821 gacatcgcaa tcggccagga acaggacgcc ctgctcaccc aaccgcgaat cttcgacctc
     8881 catgatcagg caactggcga cagttttgga gtgcggggcg ggtttaatgg tctggaatag
     8941 aggccgcagg acgccggcgg tcacctggcg gacgccggcg acaaagccgt cggcggcatg
     9001 catggcgagc atcatggcgc caaagtagtt gggctggaga accgcgtcgc gggcttcgag
     9061 cattttcatg cgcttgccgc ggtactggtg caaccgtcgg aaccggctgg cgtagttgtc
     9121 caactcttca ctgtcggccg gattcagcag acgaatgccg ttcaacgcga ccttcaagtc
     9181 gcgggcaacc tcctggatgc gaaccggatc gcccagcaga attggcaccc ccagccgcaa
     9241 cgcgtaaaac tggcgggccg ccagcagcac gcgcggttcg gttccttccg ggaacacaat
     9301 gcgcttgggg tgacgctgca atttttcgat cacgccgcta atgaatcgca tatctgcttg
     9361 gtagcgcagc gaacgccaaa gcgcaaccgg ataagcgcgc ccttaaaatg aaaagaccac
     9421 acgttgactt gcgccgctgc gccaagtact ctgtcccctg gctgaagcta aaaacatgct
     9481 cgagacgatc gaaagactcc tgattctgca agattgcgac cgcaaaattc ggcgtgagca
     9541 aggtgaactg gcacgcatcg aaccggaacg gcaaaccttt aacgccaaag ctgcggccgc
     9601 ccagtcagcg ctggaaaacg cacggcaacg cctcaagcag atcgagtcgg ctcgcaagga
     9661 ccttgaactt gaagcccagg tcaaaaagca gatgatagat cgttacgccc atcagcagtt
     9721 gttgacccga aaaaacgatg aataccgcgc tttggcctcc cagatagaaa cttgtaaaag
     9781 cgccatcttc gagcttgaga atcagcaaat cgagttaatg gaacaagccg aagcggtcca
     9841 gaaggagatt gcccatgcaa ccgcaaccgc cagcgatacc cgcaaattgg tcgaagacca
     9901 actcgctcaa ctggccgcgc gcgaggcgaa cctgcgccgg gaattggacg aactgcgcgc
     9961 ccgccgggaa accctggccg ggaacgtcga acaaaccgcg cgctcgcgct atgagcgcct
    10021 gctgcgcagc aagggtgaaa atgttctggt cggcgtgcag cacggcgtct gcggcggctg
    10081 ccacatgaaa gtgccgcccc aacttctggt aacctgccag gccgaaaaag aactggttgc
    10141 ctgcagcaac tgcggccgca tgctctacta caccccggat atggatttgg cagtgaccga
    10201 ataggatgcg gccttcatct gggcgttgct tctcggcctt cccggcctgg gccgccaacc
    10261 ccaatttccg ctccccgtga atctacgcgg gcttgctcgc acggggcggc aagagtatat
    10321 tcaagttggt tttggaaggg cggagaatcg ctttccccga atgaatccgg ggaaggagga
    10381 aagtccgaac accaaagggc gcgatgccgc gtaaccggcc ggagccgggc gctacccccc
    10441 gtgcgaacag catggaggca ggccaacctg gctcacggcg ggatacacgc gggaggtgat
    10501 gccgtgaggc atcccgacgg acagtgccgc agaaaattaa accgccgtag ccccggcaac
    10561 gaggctgcgg taagggtgaa aaggtggggt aagagcccac cgctccaagc gcaagcgcgg
    10621 aggcacggaa aaccccatcg ggtgcaaggc caaatagggg accccggagc ggcccgctcc
    10681 aggttccgaa ccgcggaacg ggtcccgggt tctggctgct gagacaaatg attctctccg
    10741 cccggcaacg ggcgcagaca aaattcggct tacagccctt ccaataccaa ttttcctgtg
    10801 gcacagtggg ccgccgttat gaggaacgca tgtttgcttc gggtgattct gctcgccggc
    10861 tgcctcgcga acgacccgtg cccgctatcg gccttgccgc ttcagcgcgc cgacgtgcct
    10921 gcagctcccg cctgggtgct gcatgtggat tgcgacaaac tgcgtccgag cgcaatgggc
    10981 cagtacctgc tggagggaat ggaaaaaccc gaaatccgag ctcgcctggg cgctttctgg
    11041 gccgcgctcc aacttgaccc gcgcaagcat ttgcgcggcc ttaccctgta cggccgcgcc
    11101 gacgccccgg cagacggcac gctgctggtc tatggagatt tccaagccgg ccaactcgag
    11161 cgcgccgccc gggcagccaa agatcacctg accacacctt accaaaagca tcgaatccac
    11221 cagtggcttg acacgagcgg aaaccgcaat ccttccgccg atcagcgcgt ctatggagcc
    11281 atgcaccatc agcgcgtgat aatcctgtcc cgccaggaga cgaacgtagc gcacgcgctg
    11341 gatgtgctgg actgcaccgt gcgcagcctc agggaaagcg gccagtgtgc acaactcggc
    11401 aaccaggcgg gcagtgtctt cctccaggcc gccgcgacca aagtggaacc gcttcccgtg
    11461 catcccgcca ccattattct ccgcctggcc cgggcggcac ggttggaaat gagcgaagcg
    11521 tcagaccggt ttacggcaac gctctcactc gaagccgacg gtaatgccac cgccacgcaa
    11581 attttgtcgg ctggccaggc cttgttgaag ctggtgaagc tgcaaccgaa tgcggcgagc
    11641 gccatgcggt tgattagcgc gatcaacctc aagcagcagg gatctgaagt cgaagctacc
    11701 ctggccgctt cagccagtga gatcattgcc ttgctgaaga atgagggaag ccagccggag
    11761 tccaagcaac caaagccgtc gtctcccaat aattaaccat cacagcagat ggccaagttc
    11821 aacccggagc cgctccacac agaagccccc ggaactgaaa cagaggacgc acgagccaat
    11881 gcggcacggc ttccaccgac tagggaatcc gctccactcg gatgcgcctg agatcactta
    11941 cgcctaactg gagcagagca gcattattat agaggcgaag gttgcgttta accgcaggaa
    12001 catccgcaaa ctggcgctgg cgatccagtt cttgaagcga aagcacgtcc agcgccacgg
    12061 gatcgcggct gaagcggagt tggttgagca ctgtcgaata gtggagcaga ccgcgctcgc
    12121 cgccctcgta ttggcaaatc agcgcatcgg tgatattgag caccacatgg tcgctcagca
    12181 cgggcaggcc gtaaatttcc gggaccgcag tagccaggct ttcgggttgg gactcgaaac
    12241 ggatcacgtt atccacacta cccgtcgcaa ggctgtagag gttgcccgca acgccggccg
    12301 cgttgtgatt gagcagcggg gtcacattga tgagcttgtt aacctggcgg gtgagcagtt
    12361 gtgagacaaa cgacttgcgc cccacactct cgcccttccg gccaaactcc aggtcacccc
    12421 agaccaggtt gcccaggatc ggactgtcag gatcataaaa gacctccaaa tcccaacccg
    12481 cttcgatgct tccagccagc cgaatgccat acttttgggc cagcgtgccg tagccagcca
    12541 ggcgcaggtc ggtgaggcgg cggtcccaga caacgatctg tttgggcggc aaccccgctg
    12601 cgagcaaacc ttcgatcacc gcagccacca ccgccggtcg cgtgccgcta tttggaccag
    12661 gtgctgaaaa caccttaatg ccaaccacat cctgggttgc gatcaggctg cgccaggctt
    12721 ctggcatggt ggcacaatgg gtcaggttgg tgatcgcaca gttgaccatc agtcgaaccc
    12781 ggtcccagcg cggcgcaaag gccgcggtgg cggtgtcatc ctgaaccacg aagatgtcag
    12841 cctggggagc ggtgctgccg ggcgaagccg gtgcaagcag ttgtgccggc agggggtgcc
    12901 cccccatcaa cccgccgaga aacaagacca gcaaggcgcc gggccgccag cgtggggtgg
    12961 cggcaaagat caaacgttcc gggcattgtg cgttacttga cacttccagc cttgaatgat
    13021 accaccctga ccaatgctga ccacaaaaaa tgcggcggcc cgcgatccgg gagcgcggat
    13081 ggcggtcagc gggccgcgtc ggtgctggag agccgggctg gccggcctgc tggcgctgct
    13141 ggccggctgc accccggcag gtccgcacgc ttttttggaa ggccgccgct tgatgaaccg
    13201 gggagaatac cagcaggcga tcgaaaagct gcgcgccgca accgtcctgc tgggcggcac
    13261 caatgcactg gcctggaacg acctgggttt ggcttaccat catgccggtg aggcggtcga
    13321 agcggagcat gcctaccggc gagcgctgac attgaaccag gatttgagcg aagcgcgcta
    13381 caatctgggc tgcctctggc tgtcgcaaaa tcgcctcgaa gccgccaaaa ctgaattcgc
    13441 cgcctacact ttgcgccgcc ccaacgaagc cccggggttc ctgaaattgg gcacggccca
    13501 attgcgagct cgggagctaa acgctgcgga gaaaagcttc cgtgaggcac ttcggctgca
    13561 gcggcaaaac ccggaagcgc tcaacgggtt gggcctgacc ttgctcgggc gcgggcaggc
    13621 aacagcggcg gcccactgct ttgaagctac gctcaaacag caagctgact atggccccgc
    13681 gctcttgaat ctggccatcg tctcgcacca gcacctgcgt aatcgccagg tggcactgga
    13741 aaaataccgg gcctatgcca cattaaagcc agtgcccgcc aatgcccagg ccgtgcaggc
    13801 aaccatacga caacttgaaa tggaacttgc ttctcctgcg cggccggcaa ccacaggccc
    13861 cacagttcca tcaatccaag tcacccctcc gccgcaaccg gttgcgcctc ccgtaaccag
    13921 cactgtggca acccataaac cagaaccgat cctcgagccc ccgaaaacca ccaccgctag
    13981 tgcggcaaaa gccagtccga ccagcgtgcc gtcgcccgct ccgattatcc ctccgccgcc
    14041 gccagctacc accggaatag tgcgcccctc cgttgaatcc gccttgcaac ctagcccgcc
    14101 agttgtcgcg aggccaatcc cggcgcaagg ttcgcccaca gaacccttga ttatcacatc
    14161 atcggtgcca gcccgggtga cagcttcgca aacggacaaa cgtggtttct tccagcgcat
    14221 caatccgctc aacctagttc gcagcaaaga gaaaacaccc ccgcagccaa cgcccctggg
    14281 gccaacccca ggtaccgagt tgccggaatc ggccgggacg gttacgacgg gagccgcgcc
    14341 gacaccgggc gtcttcaccg cgccagagcc cggcaaccaa ctgagccgct atgcctacca
    14401 ctcgccagcg gttccctccc gtggcaatcg ccccgaagcc gaggcagcgt ttacccgggg
    14461 agttcgagca caacaaacgc ggcgctggac ggaggcaaaa caggcttacc agcaagcggt
    14521 gcaactcgat cccagcctgt tcgaagcaca ttacaatctt ggcctggtgg cagccgaaac
    14581 cggcaatctg cccctggctc tgcgtgccta tgaaaccgcg ctggccctcg aaccgtcatc
    14641 aaatgatgcg cgttacaact tcgcgctggt gctcaaacga gccaactgcc cacgcgatgc
    14701 ggttaacgag ttggaaaaga tcgtcgctca ctctccaaac gaaacccgtg cccacctggc
    14761 gctgggcaat ctttacgcac agcaattgaa acagccgacc cgggcacgcc agcattattt
    14821 gaaggtgatc gaactggagc cgcagcatcc gcaggccagc gccatcaact tctggctagc
    14881 gtcgccccaa ccttagcatc gcgagctatt atgccaacct cgcggcggtc ctggccccgg
    14941 ctgatccttg cttccgcctc cccgcggcga gcggaattgc tgcgatcact gggcctcgcg
    15001 ttcgaggttc aacccgccga tgcgtcggaa acccatcatg cacagttgag cgcctgcgag
    15061 ttgtgccgga tcaatgccct ccgcaaagcg cgctgggtag ctaggaaagt tccccacgcc
    15121 ctggtgctgg cagcagacac cctggtttcc ctggaggaaa cactcctggg caagccagca
    15181 acgcaagcgg ccgccagaaa aatgcttaga caactgcaag ggcgcactca cttggtcgtg
    15241 actggagttt gtctcctgca ctggcgaacg caatgccaag tgctcttcac cgagagcacc
    15301 gctgtagcct tccgccgtct cacagattac caaatccggc aatacctgag ccgggtcaat
    15361 ccgttggata aagccggtgc ctacgccatc caggagcatg gggatctgat cattgaagag
    15421 attgccggct cctacactaa tgtcgtcggc ctccccctgg aacgcctccg gatggaactg
    15481 gcggcctggc tgggaacctg acgcatagca actctaaccg tgaaatcgcc gtccccagcg
    15541 cctccgaact cctcccaagc cgactggccg acgtcgcgaa ggacgcccga cgaacgcggt
    15601 taaccgcgtg cttgacctga gaggtcgcta ctgctaagcc tgcatctgtc accgcgttcc
    15661 ggcgctgccg ggcgcggcat ttattacaac tagttcatac tatgaagtta agcgctctac
    15721 tgtccgtgct gccggagaaa aaggtcttcg gaaaccaggc ccctgaaatc acaggagtgg
    15781 tttatgatcc tctccgagtc acccccggat gcctgtacgt cgccatcaat atctacacgc
    15841 agttggacaa gattgagatt ccggacggac atcccctcct gccggatgcc gtgcgggcag
    15901 gtgcggtggc agtcgtcctg cagcaggatc agcctgtccc tgccggggtg gtcaaaatcc
    15961 tggtgcccga ctcgcgctat gcgttggccc tgctggcggt ggagttttat cggcatccgg
    16021 cccggtccct ccaacttatt ggcgtcaccg gcacaaacgg caaaaccacc accacccacg
    16081 tcatcgaaag tctcctgatg acccgctacc gcgccggcct tatcggcacg ttgtactaca
    16141 aaatcaacgg tcgcatctgc aattcaaagg acaccacccc tgaaccgccc gatctggccg
    16201 ccatcttcac ggaaatggtc aacgagaagt gcgatttttg caccatggaa gtctcttctc
    16261 acgctgtgga tttccaccgc gtggtcggcg ttgattacca ggccgcggca tggacgaacc
    16321 tctcgcaaga tcatctcgac tggcacaaga cgatggagaa ttaccgtcac gccaagctgc
    16381 gctggctgtc cagcctcggc ccgcagcagc atgtggccct caaccatgac gatcccagcg
    16441 cccccttctt cattgagcgc gtgcgtgcgc agaaggtgct ttatggcctc aaaaacaagg
    16501 ccgacgtgac cgcccgcgat taccgtttct ctgctgacgg cacccgcttc accttggtca
    16561 cgccccgcgg tgagattgag gttcaggcca agctgcgcgg cgaattcaac ctctacaaca
    16621 tgctggcggg tgtcgccgca gttctgcata cgccgctgac gctggccgaa ctcaaacagg
    16681 gcctggaaaa gaatatcgcc gtggctggcc gcttccagcc gattgctctc gggcagccct
    16741 tctccgtcat catagattat gcccatacgc cggatgggat ggaaacggtg ctgcgggccg
    16801 cccgcgctat gaacccccag cgcctgatca cggtcttcgg ctgcggcggc gatcgcgacc
    16861 gcgccaaacg ccccctgatg gccgccgccg tcgaagcgca cagtgaccgg ttcatcatca
    16921 ccgatgacaa cccccgcacc gaagaccccg accaaatcgt gggcgacatc atggccggct
    16981 tgcaaaaaaa gcagagcggg gcacacgagg taatccacaa ccggcgcgac gccatccgtc
    17041 acgctttaga ccaagcccag cctggcgatc tggttctggt ggccggtaaa ggccacgaaa
    17101 gctctcagat cctcaaggac cgcaccatcc atttcaacga ttacgaggtg gctgaagagc
    17161 tgctccgcga gaagggctac ggcggttgac gttgcccacc tgacggctct ggctgcctgc
    17221 gcctgtgtaa tctggttctg aatgtcagtg ccatcccgga agggaagaag gtaaagtgga
    17281 atccctacct cgtaatccat caggagcctg gctgtacggc tatcctttgc gcgtcaatcc
    17341 aacaagtcaa atcacattct gacacgatcc gccagatcgt cggcggcgtg gtctcgctat
    17401 ttgcgcccta tcgaccagct cataccccag cttctgccgg tacagactcg ccaaataacg
    17461 gcggtacgct tctacgccgt caatcatcat cacacctgta attcaggtct gctgctctcg
    17521 tggccgcgat gttttcgatg tgcgaaaatg caccggtgaa agctttcagg caagactact
    17581 ttgggagttc tgccggcgga gcggacggga aatgctaata ccacttctgg cacgtaaatg
    17641 cactattctc cgctgcggta gcgtttac
//
LOCUS       DAOPQG010000077        17652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3829_length_17652_cov_13.804342, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000077 DAOPQG010000000
VERSION     DAOPQG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 17652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17652
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3829_length_17652_cov_13.804342"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3766
                     /locus_tag="PKI07_09590"
     CDS             <1..3766
                     /locus_tag="PKI07_09590"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOC51342.1"
                     /translation="FGSWRIQTQTTNVSGTAGSTREWNVDVAGAWFPYDRYIGALARN
                     ATRKNGWVNDFIIGSPEIVYGSTFVDCIPDGSGGCAANGGVFTLDLRSYGINSQTDGI
                     LLVNHGKDEGNFALSKAEEDGTWTMYVHDLSTGTASNYEQDPIAFVYIPRTDTSLISG
                     KLDGDGGIMMHSGNAPQFTTEKLANGRHRLTINGYAATNGVLIVSAEGGGTYNLDNIV
                     SYEIAPDGQSFELQSRDTPNFGLQSPYRLVGGARSPEAAFSFVFIPAPTEVGFEVWPQ
                     ANLLTAETPNAYYAGSFTNSFTVHLTAKPTADVTISGIVSSDPTEGVVCDGLTSLTFT
                     PDNWWIPQTVRVTGVDDLDADGQVAYSIVLPAAVSADARYDGLKPADVGCLNADNEAG
                     ISVSATRLTTSEGGLTATFQVALNTAPTSDDTTITLTSSDTTEGTVDPPSLTFTSLNW
                     SNRQTVTVTGVDDAEIDGDVVYTIVTGAATSSDPAYNGLDALDVTVVNLDNDAPGLSI
                     APKGMLTVSEPNTTLNLEVALTCQPAANVTVSISSSDVTEGTVSPASRTFTPANWNTP
                     QSFTLTAADDLLTDGTVGYTVTLTASSSAPGWNGLTVVLPAQTLDNEPVLTLPSGAMQ
                     YGIDWPGMVIDGQATLVDADTYDYDTGSLTVTLTANAQSDDRLGIRNQGMDWQQIGVS
                     GNTVTYSGSPIGTFAGGVGATPLVITFNASANPEAAQALARNITYHNVTNAPLQDPRT
                     VEFTLADGDGGSSTDSKQIIVSKVHISDFQYGKDAGFGVYTGASDCEIYSRVDEYASY
                     PAGTWSDPANELYGLVVHINKNTATGYDQQVLLRFDDIVGTNPGQIPPGATIVSADLL
                     LTVMNGGDGSPLYRMLIDWDPESSTWNGFGDGVQMDGVEAETTYYSQFNASDHSGSTG
                     TGRIRFSVLPDVQAWVNGTNNYGWVMPSWGPFASNDNTIFAPAEATNLVTRPQLLVRW
                     LPAEPTIKMASFRQGQDGYSGAVDTRIRELTPDTEYSTVTSVLVDYEVTPPNEDAEQA
                     ILRFDHLVGTGAGQVPPFAKIHAAELDLTSTGSNAMGDGGTFHRMLIPWEAATATWNF
                     FVEGIQADDVEAVNEPTVVAGNDTGDPNVQGGWNTFDLTADVQLWANGEANYGWAVMP
                     WPYGGDGWGFYTADHGNQAVRPQLRVYYTPTRPVMLSAEVAGAPPSSVTVLFEGEPGK
                     TYEVWRSPAVAGAGVNWTKVGEATATLSGVATFVDPAPLANSAYYRARTK"
     gene            4010..7402
                     /locus_tag="PKI07_09595"
     CDS             4010..7402
                     /locus_tag="PKI07_09595"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC51343.1"
                     /translation="MKVAMKIEKSMPGQCAVRKDWRRLGVAAVLVVSAALAPGARVAA
                     AAEAHHVIAGQIKVDQVSVVDSNLAQVTLVQQIGTFRATPRGSNRGDYNVLINPGVKA
                     QQDESGGVLMSSVTENGRDNFGTNAYPVSGIERNGSGTYRIVSFTRPSTEYNVNVAGA
                     WFPYDQYLGGLARNADGVNGGTNDTFTGSPGLELGTHFKGVAGGKSIVDLTSLGIDSR
                     TDGILLVNHAKDENNFALAQVNPLDGTWNVFVRDVGQSSYSSYEQDPVAFVFIPRTNT
                     ALISGRFRGDGTPEMFSGSTPLFTVTNVAEGRWDLRIPGHSPTNGILIISPEGGGVLN
                     GDNIVSYQAYPDGSGWEIQSRDTPICGLQTPVNEAVASFVYIPALPPEVQVEPTQGLV
                     TTGSGGTAAFSVVLSVAPTAVVTVAVASSDASKGVASPAILMFDVNDWYLPKTVTVTG
                     QVGAAEGGYQVVLSPAASADPAYQGLDPEDVAVTSLAARAAVVWPADSGTNISASPNL
                     QAWVTNTLPGNLSVTFYGREAPTVHPGPDFCISVMPDTQMYTGELGGGKKEMMIAQTE
                     WAISNRLSRNIAYVTQLGDISNNGDTPAYVKQWYNATNAMYRLEDPARTQLPDGIAYG
                     VAVGNHELTPIGWASCGTVGTTNVGTSTNYNKYFGVAHFAGREYYAGHYGTNNNNHYD
                     IFSVSGLDFLVLYFEYNTNPPPELLAWADEVLTTNAHRRAIIVTHNFGNTQTPVVWSP
                     QAQAIYNALKGHTNLFLFLGGHITGQGWREDTHNGHTIRTLVQDYQGWTNGGNGFLRL
                     LEFSPSNNVVVAQCFSPVTGEYLTDESSEFYFPYDLQPTGPGTATPFVALATNLAAPG
                     DLSSVAWPGLAANKAYEWYVAVTDQWGNTVTSPVWHFTTAVTNTPPTVVNLARVITGD
                     APATLALSATDANGDALTFQALSAPARGVIRDFDPATGTFTYWPAYGYRGSDWFLFSA
                     SDGQASSGSASINLNIVAPPDADANGLPDAWEAAYGLSDPNDDADGDGQSNLAECLAN
                     TNPTNAASVLRISEWVRQTNGHVSLAWPSIGGVRYRVQYRNGSALSGVAGAFTDIVRP
                     LTNEMDLSPYGSVSTQAFTDDFTLTGGSPTNHSRYYRVRVVR"
     gene            7426..8355
                     /locus_tag="PKI07_09600"
     CDS             7426..8355
                     /locus_tag="PKI07_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51344.1"
                     /translation="MPWGFCGAARGRERFAGCRRRRRGAWRWRAVVLAACLAALPRPP
                     AARAHGEAHLLILELTRQIEAATNNAARLYLERGELQREHGMYDGAEADYARAAQLDP
                     DLPGVDRCQARLLADLGQLAAARARFDRALERCPDDGESWVGRARVLLRLDQRRAAIA
                     DYWRGLEHLREPPPEYVVELARALVAEEQTAEALRALDAGIKKLGPILPLQGYALELE
                     LARQNPAAALARLETILARALRKETWFARRGEIFLATGKVVEARQSYHAALAAIDRLP
                     GRLQQSPSMVKLRADVKTALAGLTNAPAAGPSK"
     gene            8371..11529
                     /locus_tag="PKI07_09605"
     CDS             8371..11529
                     /locus_tag="PKI07_09605"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC51345.1"
                     /translation="MQTNWHMENARIFTRKQPPFAAAARERWTLPPASGWPRARGGSK
                     YPRPGGGAGLALAVLIALFLAGASPAHAQDRPAAANLDVVQVDWENTTNSVVVTSTLS
                     LNRLGVREGSNRGDFNVQVGESAADDYLGGAWLSSVSQNGRDSYLTNNYAVSSVHVAA
                     DGCRVSAFVPTAPQAGSAAEFNVNVAVAWFPYDEYLAGTARNSTGANGGTNDLFIGSP
                     GLELGTHFRGVAAGKSVVDLRSRGIDARTDGILLVGHAKDENNFALSQVNPNDGTWNV
                     FVRDNAQYYYTNYEQDPVAFVFIPKTNTTLISGRFNGDGSLAAFSGDTPSFMVTNFST
                     GQWELKIPGHTPAGGVLILSAEGGGTYNGDNIVSYQANAAGDGWVIQSRDTPNNGLQT
                     PVGPGGAPEAVASFVFIPAPVTARVAPDPQAQNLGVSPLLQVAVPETLAGDLSVTFYG
                     REAPTPPVGPDFCIVVMPDTQNYAAQRNDGTKEMMIAQTEWAINNRTTRNVAYVAQLG
                     DIVNNGDTPSYVSQWYNATNAMYRLENRPRTQLPDGMAYGVAVGNHEQSPNGDAVSGT
                     TSNYNRFFGVSHFAGREYYAGHYGTNNNNHYDFFSVSGLDFVVLYFEYDTNPSAEVLA
                     WGNAVLANHPHRRAIVITHYMGSAATPSKFSAQGSAIYNALKTNANLFLLLGGHVCGS
                     TGEGEGSRTDTYKGNIVRTLISDYQCRTNGGNGWMRIMDFSPSNNVVTVQTYSPWTGE
                     YETDENSEFFFPYNMHAGTSGSPGTPYAALGTNTGVSPGSVTSFQWSGLQSHTAYDWY
                     VVVTDGRGNTIEQPAWRFTTGFNVAPEVSNQLLTVVGDAPAPVALPASDPNGDALTFQ
                     LNSLPLRGRILDLDTNAGTLTYLPARGFRGSDFFTFQASDGALSSSVASLTLKVVAPP
                     DANSNNLPDAWEAAYGVSDPHADDDGDGATNLQEYWAGTNPTNAASVFQITDWQRLPN
                     GHFHFTWPSIGGVRYRVQFRNGTALSGAAGAFTDIVRLLTNEMDLSPWGTVSTQSFTD
                     DFSLTGSAPPGGARYYRVRLVQ"
     gene            11596..12594
                     /locus_tag="PKI07_09610"
     CDS             11596..12594
                     /locus_tag="PKI07_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOC51346.1"
                     /translation="MAKLKDYSDEQLRLAARLYYVDGLGQREVARLAKVSQAKVSRLL
                     AAARERGIVHISVAEYKPHNKKLEHALRREFGFRTVAVIKTPPGATDDDARRAVGYFG
                     ASVVAGLLPRKAVVAIAGGRTIRDLVRHLAEDPARRLEVVQAMGNIDSHVGSEDALEL
                     GRVLARRSGGSFVTLNSPAFVSDRKTRDALLALPPIRSVHQRLAEAQVALVGVGTLDN
                     SVFAARGVLTPRDIAELDACGAVGEICGRFFDRNGQECDSPWRGRVVSISLEQLRRIP
                     RVIGIVAGGDRSVAIAAAARGGLLKALVTEEKLGQFLFAAARASISPASESLSPGV"
     gene            complement(12767..14464)
                     /locus_tag="PKI07_09615"
     CDS             complement(12767..14464)
                     /locus_tag="PKI07_09615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC51347.1"
                     /translation="MTPAFNSGLRFKVTGAVVVLCAIYLSFAGLDHTLFWDDEALVAI
                     HARNYLQTGTWTAWTGRNLAAYGNGMLLDAQLETRDPKVQHWLAILSFKGLGVSTWTG
                     RFPFVLVGLAGLGVFVLILRREFPGQNAVKLYALTLLALSPVFLLNIRQCRYYAPCLT
                     FGLLTYYFYRRCSAGRSRADIFGLALAAALLFYSQYLVGGAFLVAIAVMHLVCHRREC
                     ARPGWINLGLAAALFLLATVPFLLTHPVWIRAEAPVDEAWYLRKPTLFWWYVRDLNAA
                     ACLPWTVTLMLIGILWWQRKHPLVRQVARPWTILGLTFLAVLSSLSFQSTRLPDLAWT
                     RYLIPVVPFLAGLTGIVLGFIAQRSRGAALALLAILLGSNLLSLSPWNTTFRWLLPAY
                     IQEVHRPFRTPYQAAVEFIRAHVSRDALVAVEPEFCAYPLMFYLDDHVRFGCLLNTNT
                     LLPRAALERLSAPLWVEEYYPDWLIAFGSSMRTAALALHFCRVHPEKGREVAYSYRLE
                     KNLEVFWFDTSRPELAFHTFGPRKNFDPGLDSVYAFRRLPAQEVYPAAAPPAPHKSE"
     gene            14589..16859
                     /locus_tag="PKI07_09620"
     CDS             14589..16859
                     /locus_tag="PKI07_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51348.1"
                     /translation="MIKQKQALSARDSRAAKRGIAGAGRWRVPGLLCLGLALATLVLF
                     LPSARHDFVNYDDRGYVTENYHVHEGLTWEGVRWAFTTYETGNWIPLTWLSHMTDCQL
                     YGLRPAGHHLTNLLLHTANVLLLFLVLTRMTGARWRSAFVAALFAWHPLHVESVAWVS
                     ERKDVLSTFFFLLALAAYARYVEGRKKAAADGMPPAAGRQLPASYFCALSCFVLGLMS
                     KPMVVTLPFVLLLLDYWPLQRLPLIRPNLQRANLLRLLAEKVPFFLLVIPASIVTLAS
                     QKSVGAVASLSSLPVGARLANALVAYARYLGKMLWPVDLAVLYPHPLHWPAGQVIAAG
                     ALLLGLTLWVARSIRSRPYLLIGWLWFLGTLVPVIGLVQAGAQAIADRYTYIPLIGPF
                     IALTWWVADSLRLGPNPRRVLGGMGGVGVLAVLGGITHQQLSHWRDSEALFGHTLKVT
                     RNNPIACNNRGFYLAEQGRLAEAIADFEAALRIDPNNADAHSNLGSALGRQGRVSEAA
                     THYLAALQTNPDHAGALYNLGKACENAGQMKDAVDCYTRAIHSQPEYADAHNSLGVVL
                     ATEGKFQEAERHLRLAVRSKPSFAEALNNLGNVLTAQNRSAEAVAFFSAALRLKPDYL
                     HAHSNLGVALVRLGRIAEALEHYDAVLRLNPDDVSVLSSKAWILSTQKEARFRNGTEA
                     VRLAARAVTLTREQDAGALDALAAAHAEAGNFAEAVSLAQRAEQCAADAGQKELAAEI
                     QQRRQLYQSGRGFREP"
BASE COUNT         3247 a         5453 c         5843 g         3109 t
ORIGIN      
        1 cttcggctcc tggcgaatcc aaacgcagac gaccaacgta tcggggacgg ccgggagcac
       61 gcgggaatgg aacgtggatg tggcgggagc ctggtttccg tatgaccgct acatcggcgc
      121 gctggcgcgc aatgccaccc gtaagaacgg ctgggtgaac gattttatta tcggttcccc
      181 cgagatcgtt tacggctcga cgtttgtgga ttgcattccc gatggctccg gcggctgcgc
      241 ggccaacggc ggcgtcttta cgctggacct gaggagttac ggcattaact cgcagaccga
      301 cggcatcctg ctggtcaacc acggcaagga tgagggcaac ttcgcgctgt cgaaggcgga
      361 agaggacggg acgtggacga tgtatgtgca tgacctctcc acgggcaccg cgagcaatta
      421 tgagcaggat ccgattgcgt ttgtgtatat cccgcggacg gatacgtcgc tgatttcggg
      481 caagctggac ggggacgggg ggatcatgat gcatagcggc aatgcgccgc agttcacgac
      541 tgaaaagctg gccaatggcc gccaccggct gaccatcaac ggctatgcgg ccaccaacgg
      601 ggtcttgatc gtttcggcgg agggcggcgg gacgtataac ctggacaaca tcgtcagcta
      661 cgagattgcc cccgacgggc aaagttttga gttgcagtcg cgcgacacgc ccaattttgg
      721 gttgcagtcg ccgtaccggc tggtcggcgg cgcgcgctct ccggaagcgg cgttttcgtt
      781 tgtgttcatc ccggcgccga ccgaggtggg atttgaggtg tggccgcagg cgaacctgct
      841 gaccgccgag acgcccaacg cgtattacgc cggcagtttt accaattcat tcacggtgca
      901 cctgacggcc aagcccacgg cggacgtgac gatcagcgga atcgtttcga gcgacccgac
      961 tgagggggtg gtctgcgacg gtttgaccag cctgacgttc acgccggaca actggtggat
     1021 cccgcagacc gtgcgggtga cgggggtgga cgacctggat gcggacgggc aggtggccta
     1081 ttcgatcgtg ctgccggcgg cggtgagcgc ggatgcgcgg tatgacgggt tgaagccggc
     1141 ggatgtgggc tgcctcaacg cggacaacga ggcggggatc agcgtgagcg cgacgcgcct
     1201 gacgaccagc gaggggggcc tgacggcgac cttccaggtg gccttgaaca cggcgccgac
     1261 ctcggacgat acgacgatca cgctcacctc gagcgatacg acggaaggaa ccgttgaccc
     1321 gccgagcctt acgttcacgt cgttgaactg gtcgaaccgg cagacggtga cggtcacggg
     1381 cgtggatgac gcggagattg acggggatgt ggtttacacg atcgtgacgg gggcggcgac
     1441 gagctcggac ccggcctata acgggctgga cgcgctggac gtgacggtgg tcaacctgga
     1501 caacgacgcg ccgggtttgt ccatagcgcc caagggaatg ttgacggtga gcgaacccaa
     1561 taccacgctc aatcttgagg tggcgttgac gtgccagccg gccgccaacg tgacggtcag
     1621 catcagctcg agcgatgtga ccgagggcac ggtgagcccg gcgtcgcgga cgtttacgcc
     1681 ggccaactgg aatacgccgc agagtttcac gctgaccgcc gcggatgacc tgctgacgga
     1741 cggcacggtg ggctacacgg tgacgctgac ggccagttcg agcgcgccgg gttggaacgg
     1801 attaacggtg gttctgccgg cgcagacgct ggacaacgaa ccggtgctga cgctgccgtc
     1861 gggggcgatg caatacggga ttgactggcc ggggatggtg attgacgggc aggcgaccct
     1921 cgtggatgcc gatacgtacg actacgacac cgggtcgttg accgtgacgc tgacggccaa
     1981 tgcccaaagc gatgaccggc tgggaatccg caaccaggga atggattggc agcagatcgg
     2041 ggtctcgggc aacaccgtga cctacagcgg gtcgccgatc gggacctttg ccggcggagt
     2101 aggcgccacg ccgctggtga ttacgttcaa tgcttcggcc aacccggaag cggcgcaggc
     2161 gctggcgcga aacattacct accacaacgt gaccaacgcg cccttgcagg atccgcgcac
     2221 ggtggagttt acgctggccg atggcgatgg cggcagcagc accgacagca agcaaatcat
     2281 cgtgtcgaag gtgcatatca gcgacttcca gtacgggaag gacgcggggt ttggggtcta
     2341 tacgggcgcc agcgattgcg agatctattc gcgtgtggat gaatacgctt cctacccggc
     2401 gggcacctgg tcggacccgg ccaatgagct ttacgggctg gtggtgcata tcaacaagaa
     2461 tacggcaacc ggctacgacc agcaggtgct gctgcggttt gacgacatcg tgggaaccaa
     2521 tccgggccag atcccgccgg gcgccacgat cgtatcggcg gatctgctgc tcaccgtaat
     2581 gaacggcgga gatggcagcc cgctctaccg gatgctgatt gattgggatc cggaatcctc
     2641 gacctggaat ggttttgggg atggcgtgca gatggacggg gtggaggcgg aaacgaccta
     2701 ctactcgcag tttaatgcgt cggatcactc gggcagcacg ggaacgggcc ggatccggtt
     2761 cagcgtgttg ccggacgtgc aggcctgggt gaatggcacg aataactacg ggtgggtgat
     2821 gccgtcctgg ggcccgttcg ccagcaacga caatacgatc ttcgccccgg cggaggcgac
     2881 gaacctggtg acgcggccgc aattgctggt gcggtggctg cccgcggagc cgacgatcaa
     2941 gatggcgagc ttccggcagg gccaggatgg ctattcgggc gcggtggata cgcgcatccg
     3001 ggagctgacg ccggatacgg agtattcgac ggtcacgtcc gttctcgtgg actacgaagt
     3061 gactccgccc aatgaggacg ccgagcaagc aatcctgcgg tttgaccacc tggtcgggac
     3121 cggggcgggg caggtgccgc cgtttgcgaa gattcacgcg gcggaactgg acctgaccag
     3181 caccggctcc aatgcgatgg gtgacggcgg gaccttccac cggatgctga ttccgtggga
     3241 agcggcgacg gccacctgga acttctttgt ggaaggcatc caggccgacg acgtggaagc
     3301 ggtgaatgag ccgacggtgg tggcgggcaa cgacacgggc gatccgaacg tgcagggcgg
     3361 gtggaatacc tttgacctga ccgcggacgt gcaactgtgg gccaacggcg aggccaacta
     3421 cgggtgggcg gtgatgccgt ggccgtatgg cggggatggc tggggcttct acacggcgga
     3481 tcacggcaat caggcagtgc gcccgcaact gcgggtttat tataccccga cgcggccggt
     3541 gatgttatcg gccgaagtgg ccggagctcc tccctccagt gtgacggtgc tcttcgaggg
     3601 tgagccgggc aagacctacg aggtctggcg cagcccggcg gtggccggag cgggagtcaa
     3661 ctggacgaag gttggcgagg cgacggcaac cttgagcggg gtggcgacct ttgtggatcc
     3721 cgcgcccctg gccaatagcg cgtattatcg ggcgcgcacg aaatagcggc cggccgttgc
     3781 tcataatgcg gtaaaatcga gcgggacggt ggggtcaccg tcccgctttc tttagcagag
     3841 gccgccgccg ggccgggtct tttgcccgga ggccgggccg aaaacaacag gatcgggagc
     3901 cgcccggctg ggagcgaggc ggcgacgggt tatccggggg agtgggcatt gccggctggc
     3961 gaggcccgcg cacggtgctg gcgccgcgcg cgataaaaag tttaagcgta tgaaagtagc
     4021 aatgaagatt gagaaaagca tgccgggtca gtgcgcggtg cggaaggact ggcgccggct
     4081 tggcgtggcg gcggtgctgg ttgtgagcgc cgccctggcg ccgggcgcac gggtcgccgc
     4141 cgcggctgag gcgcaccacg tcatcgccgg tcaaatcaaa gttgaccagg tttccgtggt
     4201 ggattcgaac ctggcgcagg taaccctggt tcaacagatc gggactttcc gcgccacccc
     4261 ccggggcagc aaccggggag attacaacgt gctgatcaac cccggcgtca aggcccagca
     4321 ggatgaaagc gggggcgtgc tgatgagcag tgtgaccgag aacgggcgtg ataactttgg
     4381 caccaatgcc tacccggtca gcggcattga acgaaatggc agcggcacct accggatcgt
     4441 gagtttcacg cgcccctcca ccgagtacaa tgtcaacgtg gcgggcgcct ggttcccgta
     4501 tgatcaatac ctgggcggcc tggcccgcaa tgcggatggc gtgaacgggg gaacgaacga
     4561 cacgttcacc ggctcgccgg ggctggagtt gggcacgcat ttcaagggtg tggcgggcgg
     4621 aaaatccatc gtggacctga cttcgctggg cattgactcc cgcaccgatg ggattctttt
     4681 ggtgaatcac gccaaggatg aaaacaattt tgcgctggcg caagtcaacc ccctggatgg
     4741 cacgtggaat gtgtttgtcc gggacgtcgg ccagtcgtcg tatagcagct acgagcagga
     4801 cccggtggcg tttgtcttta ttccccggac caatacggcg ctgatttccg gccgcttccg
     4861 gggcgatggc acgcccgaga tgttcagcgg cagcaccccg ctgtttaccg tcaccaatgt
     4921 ggcggagggc cgctgggatc tgcggattcc cggccattcg ccgaccaatg gcatcctgat
     4981 tatttcgccg gaaggcggcg gcgtgctcaa cggcgataac atcgtcagct accaggccta
     5041 cccggacggc tcgggctggg agattcaatc gcgcgacacc cccatctgcg gtttgcagac
     5101 gccggtgaac gaagcggtgg cttcgtttgt gtatatcccc gcgctgccgc ccgaagtcca
     5161 ggtcgagccc acccagggcc tggtgaccac cggcagcggc gggacggcgg ctttctcggt
     5221 ggtgctttcc gttgcgccga cggcggtggt gaccgtggcg gtggcttcga gtgatgccag
     5281 caaaggcgtc gccagcccgg ccatcctgat gtttgatgtg aatgattggt atctgccgaa
     5341 gaccgtcacg gtgaccggcc aggtcggcgc ggcggagggc ggctaccagg ttgtcctgtc
     5401 gcccgccgcc agcgcggacc cggcctacca gggcctggac ccggaggatg tggccgtcac
     5461 ttcgctggcc gcgcgggcgg cggtggtctg gccggcggat tcaggcacga acatcagcgc
     5521 ctcgccgaac ctgcaggcgt gggtgaccaa taccctgccc ggcaacctgt cggtgacgtt
     5581 ttatggacgg gaagcgccca cggtgcatcc cgggccggat ttctgcattt cggtcatgcc
     5641 ggatacccag atgtacacgg gagaattggg cggcggaaag aaagagatga tgattgccca
     5701 gaccgaatgg gccatcagca accgtctttc gcgcaatatc gcctacgtga cccagctcgg
     5761 ggatatttcg aataatggcg acaccccggc ctacgttaaa cagtggtata acgcgaccaa
     5821 cgccatgtac cgcctggagg acccggcgcg cacccaactg cccgatggca tcgcctacgg
     5881 cgtggcggtg ggcaaccacg agctgacgcc tatcggctgg gcgtcgtgcg gcacggtggg
     5941 aacaacgaat gtgggcacct ccaccaacta caacaagtat ttcggcgtgg cgcattttgc
     6001 cgggcgcgaa tactacgcgg ggcactatgg gacgaacaat aataaccact acgatatctt
     6061 cagcgtcagc gggctggact tcctggtgct gtatttcgag tataacacca atccgccccc
     6121 ggaattgctg gcctgggccg acgaggtgtt gaccaccaac gcccaccggc gcgccatcat
     6181 cgtgacgcac aactttggca atacccagac gccggtggtt tggagccccc aggcgcaggc
     6241 catctataac gcgctcaaag gccacaccaa cctgttcctg tttttgggcg gccatatcac
     6301 cggccagggc tggcgcgagg atacccacaa tggccatacc atccggaccc tggtgcagga
     6361 ttaccagggc tggaccaatg gcggcaacgg gttcctgcgg ctgctggaat tctcccccag
     6421 caataacgtc gtggttgccc agtgcttttc gccggtgacg ggcgaatacc ttaccgacga
     6481 gtccagcgag ttctacttcc cgtacgacct gcagccgacc ggccccggca ccgcgacccc
     6541 gttcgtcgcg ctggcgacga acctggcggc cccgggcgac ttgagctcgg ttgcgtggcc
     6601 cgggctggcg gccaacaagg cttatgaatg gtatgtggcg gtgaccgatc aatgggggaa
     6661 taccgtcacc agcccggtct ggcactttac cacggccgtg acgaataccc cgcccaccgt
     6721 ggtcaacctg gcgcgggtga tcaccggcga cgccccggcc accctagcgc tgtcggcgac
     6781 tgatgccaat ggcgatgcgc tgacattcca ggccctctcg gcgccggcgc ggggcgtcat
     6841 ccgggacttt gacccggcca cgggcacgtt cacgtattgg ccggcctacg ggtatcgggg
     6901 gtccgactgg ttcctcttca gcgccagcga tggacaggcc agcagcggct cggcctccat
     6961 aaaccttaac atcgtcgcgc ccccggacgc tgacgccaac ggcctgccgg atgcctggga
     7021 ggccgcctat gggcttagcg atccgaacga cgatgcggat ggcgacggcc aaagcaacct
     7081 ggccgagtgc ctggcgaaca ccaatcccac caatgcggcc tccgtgctgc ggatttcgga
     7141 gtgggtgcgg cagaccaatg gccacgtcag cctggcctgg ccctcgattg gcggcgtgcg
     7201 ctaccgcgtc cagtaccgca acgggtcggc gctcagcggc gtcgcgggcg cttttacgga
     7261 cattgtgcgg ccgctgacca acgagatgga tctcagcccc tatggcagcg tctcgacgca
     7321 ggcgttcacc gacgacttca ccctgaccgg cggttcgccg accaaccact cccgctatta
     7381 ccgggtccgg gtggtgagat gatggaggcg gccggccgga ctcttttgcc ttggggtttt
     7441 tgcggcgcgg cgcgcggccg ggagcgtttc gccggttgcc gccgccggcg gcgcggcgca
     7501 tggcgctggc gggccgtcgt gctcgccgcg tgcctggcgg cgttgccgcg gcctcccgcc
     7561 gcgcgcgccc acggggaagc ccacctgctc attctggagc tgacgcggca aatcgaggcg
     7621 gcgacgaaca acgcggcgcg gctctacctc gaacgcggcg aattgcagcg cgagcatggg
     7681 atgtatgacg gcgccgaggc cgactacgcg cgcgcggcgc agcttgatcc ggacctgccc
     7741 ggggtggacc gctgccaggc ccggcttttg gccgatctcg ggcagttggc ggcggcgcgc
     7801 gcccggtttg accgggcgct ggagcgttgt cccgatgatg gcgaaagctg ggtggggcgc
     7861 gcgcgcgtgc tgctccggct ggaccagcgg cgggcggcca tcgcggatta ctggcgcggg
     7921 ctggaacact tgcgcgagcc gccaccggag tacgtggtgg aattggcgcg ggcgctggtt
     7981 gccgaagaac agaccgcgga agcgctgcgc gcgctggacg cgggcatcaa aaagctggga
     8041 ccgatcctgc cgctccaggg gtatgcgttg gaactggaac tggcgcgcca gaacccggcg
     8101 gcggcgctcg cccgcctcga gacgattctc gcccgggcct tgcgcaagga aacgtggttt
     8161 gcgcggcgcg gcgagatttt tctggcgacg ggaaaggtgg tcgaagcccg gcaatcctat
     8221 catgccgctc tggcggccat tgatcgcctg ccgggccgat tgcagcaaag cccttccatg
     8281 gtgaagctgc gcgcggacgt taaaaccgcc ctggcggggc tgaccaacgc gccggcggcg
     8341 ggcccatcaa aataagaacc aaggttgcga atgcaaacaa actggcacat ggaaaacgcg
     8401 aggattttta cgcgaaaaca gccgccgttc gcggctgccg cccgggagcg gtggacgctc
     8461 ccgcccgctt ccggatggcc gcgcgctcgc ggcggctcga aatatccgcg ccccggcgga
     8521 ggcgccgggc tggcgctggc ggtcttgatc gcgctgttcc tggccggggc gtctccggcc
     8581 catgcccagg accgccccgc ggcggccaac ctggacgtgg tgcaggtgga ctgggagaat
     8641 acgaccaact ccgtcgtggt gacctcgacg ctctcgctca accggctggg ggttcgcgag
     8701 gggtccaacc ggggagattt taatgtccag gtgggcgaat cggccgctga tgattacctg
     8761 ggcggcgcct ggctttcctc ggtcagccag aatggccggg acagttatct caccaataac
     8821 tacgccgtca gctccgttca cgtggccgcg gacggctgcc gggtcagcgc ctttgtgccg
     8881 acggcgccgc aggccggctc ggcggctgaa tttaacgtca acgttgcggt ggcctggttt
     8941 ccttatgatg agtacctggc ggggacggcg cgcaattcaa cgggcgccaa cggcggcacc
     9001 aatgacctgt tcatcgggtc gccgggccta gagttgggca cgcacttccg gggggtggcc
     9061 gcgggcaaat ccgtggtgga cctgcggagc cgggggattg atgcccggac cgacgggatt
     9121 ttgctggtcg gccacgccaa ggacgagaac aatttcgcgc tgtcgcaggt caaccccaac
     9181 gacggcacct ggaatgtgtt tgtgcgggac aatgcccagt actactacac caactatgag
     9241 caggacccgg tggctttcgt gttcatcccc aagaccaata cgacgctgat ttccgggcgg
     9301 ttcaacggcg atggcagcct ggccgctttc agcggagaca ctccgtcatt catggtcacg
     9361 aacttcagca ccggccagtg ggaactcaaa atcccgggcc atacccccgc cggcggcgtc
     9421 ctgattcttt ccgccgaagg aggcggcact tacaacggag ataatattgt cagctaccag
     9481 gcgaatgccg ccggtgatgg ctgggtgatc caatcccgcg acacgccgaa caacgggttg
     9541 caaaccccgg tcgggccggg cggcgctccc gaagcggtcg cgtcgttcgt ctttatcccg
     9601 gcgcccgtca cggcgcgggt ggcgcccgat ccccaggccc agaacctggg agtgtcgccg
     9661 cttttgcagg tggccgttcc cgagaccctg gcgggcgatc tcagcgtgac cttttacggg
     9721 cgcgaagcac ccaccccgcc ggtggggccg gatttctgca tcgtggtcat gcccgacact
     9781 cagaattacg cggcgcaaag aaacgacggc acgaaggaga tgatgattgc ccagacggaa
     9841 tgggcgatta acaaccggac gacccgcaac gtcgcgtacg tggcccaact cggcgatatt
     9901 gtcaacaacg gggacacccc ctcctacgtc tcgcaatggt ataacgcgac caatgccatg
     9961 taccgcctgg aaaaccggcc gcggacgcag cttccggatg gcatggccta tggcgtggcc
    10021 gtgggcaacc acgagcaatc gccgaatggg gacgccgtca gtggaaccac cagcaactat
    10081 aaccggtttt ttggcgtgtc gcactttgcc gggcgcgagt attacgcggg ccactatggc
    10141 accaacaaca acaatcatta tgattttttc agcgtcagcg ggctggactt tgtcgtgctg
    10201 tatttcgagt acgataccaa cccttcggcg gaagtgctgg cgtggggcaa tgcggtgctg
    10261 gccaaccacc cccaccggcg cgccatcgtt atcacgcatt atatgggttc cgccgccacg
    10321 cccagcaagt tcagcgccca gggttcggcg atctacaacg ccctcaagac caacgccaat
    10381 ctctttctac tgctgggcgg ccacgtttgc ggcagcaccg gcgaaggcga gggctcgcgc
    10441 acggacacct acaagggcaa catcgtccgc acgctcatct ccgattacca gtgccgcacc
    10501 aatggcggca atggctggat gcgcatcatg gatttttcgc ccagcaacaa tgtggtcacc
    10561 gtccagacct actcgccctg gaccggggaa tacgagaccg acgagaacag cgagtttttc
    10621 ttcccctata acatgcatgc gggaaccagc ggttcccccg ggacgcccta tgccgcgctg
    10681 ggcaccaaca ccggcgtcag ccccggcagc gtcaccagtt tccagtggtc cggattgcag
    10741 agccataccg cctatgactg gtacgtggtg gtcaccgacg ggcggggcaa caccatcgag
    10801 caacccgcct ggcgcttcac caccggcttc aatgtcgccc cggaggtcag caaccagctc
    10861 ctgaccgtgg tcggcgatgc cccggccccg gtggcgttgc cggcctccga ccccaacggc
    10921 gacgcgctga cgtttcagct caattccttg ccgctccgcg gccggattct ggacctggat
    10981 accaacgccg gcacgctgac ctatctgccc gcccgcggct tccgggggtc cgattttttc
    11041 actttccagg ccagcgacgg ggcgctcagc agctccgttg cgtccctgac gctcaaagtg
    11101 gttgcgccgc cggatgccaa cagcaacaac cttcccgatg cctgggaagc tgcctatggc
    11161 gtcagcgatc cccacgcgga tgacgacggc gacggcgcca ccaacctgca ggagtattgg
    11221 gccggcacca atcccaccaa cgcggcctcc gtgtttcaga ttaccgattg gcagcgcctc
    11281 cccaacgggc atttccactt cacctggccc tcgattggcg gggtgcgcta ccgtgtccag
    11341 ttccgcaacg ggacggcgct cagcggcgcc gcgggcgcct tcacggacat cgtgcggctg
    11401 ctgaccaacg agatggatct cagcccctgg ggcaccgtct cgacgcagag tttcaccgac
    11461 gacttttccc tgaccggcag cgcgccgccc ggaggcgccc gctactaccg ggtccggctc
    11521 gtgcagtaag cggccggttg aagccgccgg gcccgcccgc gccggaccgg caggcctggt
    11581 ccgcgggacg cgagtatggc aaagttgaaa gactattctg atgagcaact gcgcctggcg
    11641 gcgcgcctgt actatgtgga cgggctgggc cagcgcgaag ttgcccggct ggcgaaggtt
    11701 tcccaggcca aagtgtcgcg gctgctggcg gcggcgcggg agcgcggcat cgtgcatatt
    11761 tccgtggcgg aatacaagcc gcacaacaag aagctggagc acgccctgcg ccgggaattc
    11821 ggctttcgga cggtcgccgt aatcaagacc cctcccggcg cgaccgacga cgatgcccgg
    11881 cgcgccgtcg gctactttgg cgcttcggtg gtggcgggcc tgctgccgcg gaaagccgtg
    11941 gtcgccattg ccggcggccg caccattcgg gacctggtgc gccacctggc cgaagacccg
    12001 gcccggcgcc tggaagtcgt ccaggccatg ggcaatattg attcccatgt cggttctgaa
    12061 gacgcgcttg aattgggccg ggtgctcgcg cgccgcagcg gcggctcatt cgttaccctg
    12121 aacagcccgg cgttcgtttc cgaccgcaag acccgcgacg ctttacttgc tttgcccccc
    12181 atccgctcgg tccatcaacg cctggccgaa gcccaggtcg cgcttgtcgg cgttggcacc
    12241 ctcgacaatt ccgtctttgc cgcccgcggg gttttaacgc cccgcgacat cgccgagctg
    12301 gacgcctgcg gcgccgtggg ggaaatttgc ggccgctttt tcgaccggaa cggccaggaa
    12361 tgcgactcgc cctggcgcgg ccgtgtcgtc agcattagcc tggagcaact gcgccggatc
    12421 ccccgggtca tcgggatcgt ggccggcggc gaccgttctg ttgccattgc cgccgccgcc
    12481 cggggcggcc tgctcaaagc cctggtcacc gaggagaaat tagggcaatt cctgttcgcc
    12541 gccgcccggg cctcaatctc tcctgcatcc gagagcctct cccccggagt ttgagccgtt
    12601 ccggcaaccg gatggtggaa agacagtgag gaagagcggg cgaggagaac agcgctcctg
    12661 tggcgtgctt ccggccagtc cccaagtccg ggcggccccg gcagggggga ccgcgctcca
    12721 gccagcgggg tggtctggag ttgcgatttt ctgaccgcca ggttgattac tcggatttat
    12781 gcggcgccgg tggcgccgcc gcgggataga cctcttgcgc gggcaggcgg cgaaaggcgt
    12841 aaaccgaatc gagcccggga tcgaagtttt tccgggggcc gaaggtatgg aacgccagtt
    12901 ccgggcggct ggtgtcaaac cagaagacct ccaggttctt ttccagccgg taggaatagg
    12961 cgacctcccg gcccttttcg gggtgcactc ggcagaagtg cagggccagc gcggcggtcc
    13021 gcatgctgct gccaaaggca atcagccaat cagggtaata ctcctcaacc catagcggcg
    13081 ctgacaggcg ctccagcgct gcgcggggca gcagggtgtt ggtattcagc aggcagccga
    13141 agcggacgtg gtcgtcgaga tagaacatta gcgggtaagc gcagaactcc ggttccacgg
    13201 cgaccagcgc gtcgcggctg acatgggcgc ggatgaactc gacggccgcc tgataaggcg
    13261 tgcgaaacgg gcgatgaacc tcctgaatat aagcgggcaa gagccagcgg aaggtcgtgt
    13321 tccagggcga gagggaaagc aggttgctgc ccagcaggat tgccagcagc gcgagggcgg
    13381 cgccccggga tcgctgcgcg atgaacccca gaacgatgcc cgtcaacccg gccaggaacg
    13441 gcaccacggg aattaggtag cgggtccagg ccaggtccgg aaggcgtgtg gattggaagg
    13501 ataaagagga gaggaccgcc agaaacgtca gccccaggat tgtccatggc ctggcgacct
    13561 ggcgcaccag gggatgcttt cgctgccacc acaaaatccc gatgagcatc agcgtcaccg
    13621 tccagggcag gcaggcggcc gcattgaggt cgcgcacata ccaccaaaag agcgtgggct
    13681 tgcggaggta ccacgcttca tccacgggcg cttcggcgcg aatccagacg gggtgggtca
    13741 ggaggaatgg aacggtggcc aggaggaaga gcgcggccgc caggccgaga ttaatccatc
    13801 ctggccgggc gcattcccgc cggtggcaaa ccaggtgcat gaccgcaatc gcaaccagga
    13861 aggcgccccc taccagatac tgggagtaga acagcagcgc ggccgcgagg gccagtccga
    13921 agatgtccgc gcggctccgg cccgcgctgc agcggcggta gaaataatac gtcagcaatc
    13981 caaaagtaag gcacggcgcg taatagcggc attggcggat gttgaggaga aagaccgggg
    14041 aaagggccag gagcgtgagc gcatagagct tcaccgcgtt ttggcccggg aactcacggc
    14101 gcaaaatcag gacgaatacc cccaggcccg ccagtcccac gaggacgaag gggaatcggc
    14161 cggtccaggt ggaaacgccc aaacctttga acgacagaat cgccagccaa tgttgcacct
    14221 tgggatcccg cgtctccagt tgggcgtcca ggagcatccc gttgccgtac gcggccagat
    14281 tgcgccccgt ccacgcggtc caggtgcccg tctgcaaata gttccgggca tgaatggcga
    14341 ccagcgcctc gtcatcccag aacagcgtat ggtccaggcc ggcaaagctc agatagatgg
    14401 cgcatagaac caccaccgcg ccggtgacct tgaagcgcaa gccggaattg aaagcgggcg
    14461 tcatcgcgga tgctttgccc caatctgtgt gaaggcgggc tcgcgggtca atcttcaacc
    14521 gcccgttccc ttttttgttt ctcccccgcg agttttaagt tagttgttcc cggcagcgac
    14581 aattcctgat gattaaacaa aagcaagcgc tctcggcccg ggattcccgg gcggcaaagc
    14641 gcgggatcgc cggggccgga agatggcgcg ttccgggttt gctttgcctg gggctggcgc
    14701 tggccacgct ggttttattc ctgccatcgg cgcggcatga cttcgtcaac tatgacgacc
    14761 gcggctacgt gacggagaat tatcacgtcc acgagggcct gacctgggaa ggggtgcgct
    14821 gggcttttac cacctacgaa accggcaact ggattcccct gacctggctg tcgcacatga
    14881 ccgattgcca gttgtatgga ctccggcccg ccggccatca cctgaccaac ctgctgcttc
    14941 acaccgccaa tgtcctgctg ctttttctgg ttctgacccg catgaccggg gcccggtggc
    15001 gaagcgcgtt cgtggccgcg ttatttgcct ggcacccgtt gcacgtggaa tccgtggcct
    15061 gggtttccga acgcaaagac gtgctgagca ccttcttttt tctgctggcg ctggcggctt
    15121 atgcgcgtta tgtcgaaggc cgaaagaagg ccgccgcgga cgggatgcct cccgcggccg
    15181 ggcgccagct tcccgccagc tacttctgcg ccctttcctg ctttgtcctg ggcttgatga
    15241 gcaagccgat ggtggtgacg ctgccgtttg tcctgctgct gctggactat tggccgttgc
    15301 agcgtctccc gcttatccgc cccaacctcc agcgcgcgaa cctgctccgc ttgctggcgg
    15361 agaaagtgcc gttcttcctg ctcgtcattc cggcgagcat agtaaccctt gccagccaga
    15421 aaagcgttgg cgcggtagcc tcgttgagca gtttgccagt cggcgcccgc ctcgccaatg
    15481 ccctcgtcgc gtatgcccgc tacctcggca aaatgctctg gccggtggat ttggccgtgc
    15541 tctaccccca cccgcttcac tggccggcgg gccaggtgat cgcggccggc gccctgctgc
    15601 tggggctcac gctctgggtc gcgcggtcaa tacgatcgcg cccctatttg ttgatcggct
    15661 ggctctggtt cctgggcacg ctggtgccgg tcattggact ggtgcaggcg ggcgcccagg
    15721 cgatcgctga ccgctacacc tacattccgt tgatcggccc cttcatcgcc ctgacctggt
    15781 gggttgcgga ttcattgcgc ctcgggccca acccgcggcg ggttctgggc gggatgggcg
    15841 gcgtgggagt gctggcggtt ctgggaggaa taacccatca gcagctttcg cattggcggg
    15901 atagcgaggc cctgtttgga cacaccctca aggtgacccg gaacaacccc atcgcctgca
    15961 ataaccgggg tttttacctg gccgagcagg gaagactggc cgaggccatc gcggactttg
    16021 aagccgccct gcggattgat ccgaacaatg ccgatgccca cagcaacctg ggatcggcgc
    16081 tgggcaggca aggaagggtg tcggaagccg cgactcacta cctggcggcg ctgcaaacga
    16141 atccggatca tgccggggcg ctttacaacc tggggaaagc ctgcgaaaac gccgggcaga
    16201 tgaaggatgc cgtggattgc tatacgcggg ccatccattc ccagcccgaa tatgcggatg
    16261 cccacaacag cctgggggtt gttttggcga cggaaggcaa gtttcaggag gccgagcgcc
    16321 atctgcggct cgccgtgcgt tccaaaccga gtttcgccga agccctgaat aacctgggga
    16381 acgtgctgac cgcccaaaac cggagcgctg aagcggtcgc ctttttctcc gccgctctgc
    16441 ggttgaaacc ggactacctc cacgcacaca gcaaccttgg ggtggccttg gtgcgcctgg
    16501 gcagaatcgc cgaggctttg gagcattacg acgcggtttt gcggctcaac cccgacgatg
    16561 tgagcgtctt gtccagcaaa gcctggattc tctcgacaca gaaggaggcg cgattccgca
    16621 atggcactga ggcggttcgc ttggcggcgc gcgcggtcac actgacccgg gagcaggatg
    16681 ccggggctct ggacgcactg gcggcggcac atgctgaagc aggaaacttt gcggaagccg
    16741 taagcctggc gcaacgcgcc gagcaatgcg ccgccgacgc cgggcagaag gaactggcgg
    16801 ccgagataca acaacgccgg cagctctacc aatccggccg cggttttcgg gaaccttagc
    16861 cggaacgagt cagatttcac ctcccattcg attccacgaa acgcgggagc ccttggcttt
    16921 gcgggcgaaa gacattggga tccacagcca gaccgttaag ctcgccaacc aaacaatctg
    16981 ctgaagctac ccgctggcct gtcaggcccg cttcggcccc agtggaacga gggcgcacgg
    17041 ttgaaatgcg caacgtcaac gtttcagata acgctttcgc gaatccaaac gccatccagg
    17101 cgcccgtccg cccttgaaaa accctctttt ggcagccttt gtgtcataac tagctgcttt
    17161 tgaggagctt agtaattacc ctattgtcct ctctcatcgt tttggccttt ccagacatcc
    17221 gtatccatta cccaagtccc ttggattccc cttttccctg cgccgtgtac aaggggtgta
    17281 caagggatgc tcaccgggag tccataagat tttaacaggt acacctgatg ggcatccctt
    17341 gtacacccct tgtacacggc gcaggggaaa ggggtatgcg aggtaagtgc aaggcaggtc
    17401 caaagtaagc cgaggacggc atgattcaga ttttgcctct attttacccc tctctatact
    17461 taaaccattg attttaccgg cgaaagactc ggagctcagc agccatgcca ttgaattcgc
    17521 caacccaggg gttcgccagg gctgcagctt ggacggccag ctccgtccgg gctccgatga
    17581 agcgagagcg ggcggttgga cgggtagtgt cctaaaagtt ctgcaaaaag cagcgggtca
    17641 tggtaagcca ct
//
LOCUS       DAOPQG010000078        17337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_3921_length_17337_cov_14.323284, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000078 DAOPQG010000000
VERSION     DAOPQG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 17337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17337
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3921_length_17337_cov_14.323284"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..545
                     /locus_tag="PKI07_09625"
     CDS             279..545
                     /locus_tag="PKI07_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51349.1"
                     /translation="MIRRLIRITALLVLVAALAAWVAAGANRGWTKTSVPVQRTDEVT
                     GLTVDDYQKRFVPGVDFLVAAVLGAGVLAASSFLFRKPQPQPSN"
     gene            568..1182
                     /locus_tag="PKI07_09630"
     CDS             568..1182
                     /locus_tag="PKI07_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOC51350.1"
                     /translation="MKLLSRYLLGAGLLVNLTVLAAPLNFDFKDPKGVNNATFKLDAP
                     LEAISGNATGISGTVAFDPQAPAATTGKIVVATSSLRVGNPIQQEHMLGAQWLDAAKY
                     PEISFTARELKNVKTSGDTTTAEVVGTFSLKGISKTLTVPVKLTYLKDKLGQRVPDLK
                     GDLLVIRANFSIKRSDFGIMPGQAEEKVAETIELTLSIAGAAPR"
     gene            1412..3202
                     /gene="lepA"
                     /locus_tag="PKI07_09635"
     CDS             1412..3202
                     /gene="lepA"
                     /locus_tag="PKI07_09635"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOC51351.1"
                     /translation="MDTAHIRNFSIIAHIDHGKTTLSDRLLHRTGTITTREMEDQLLD
                     SMDLEKERGITIKAHPVTMLYQAGNGETYELNLIDTPGHVDFSYEVSRSLSACEGALL
                     IVDAAQGVEAQTVANVHLAMKQNLAIIPVINKIDLPHADVAQAKRQLEDILAIAGDSA
                     ILASAKEGIGIDQILEAIVERIPAPKPTGAPSLQALGFDCYFDTYRGVVTHVRVFNGE
                     LKPGMQVKLLHSGKNVEVKEVGSFNPKPYVRERLECGETGYMTANIKSPLEVKMGDTI
                     TDARHPAPALPGFKEIHPMVFSGIYPINTGDYEHLKANLAKLQLNDSAFVYQPETSVA
                     LGFGFRCGFLGLLHLEIVQERLRREYNMDIIATYPSVIYRVKLTDGTEVQIDNPAYLP
                     PVAQIESISEPMVRAFVICPNEYIGDMMALITEKRGAVDHTETLDSRRVMLTSLLPLN
                     EILIDFHDRIKSITRGYGSMDYEPADYQASNMVKLDLLVNGEAVDAFSCIVHRDKAEG
                     RGRALAARLKEVIPRQLYAVAIQAAIGGKIIARETVSALRKDVTAKCYGGDITRKRKL
                     LEKQKEGKKRMKSFGSVNIPQEAFIEVLKA"
     gene            3206..4492
                     /gene="lepB"
                     /locus_tag="PKI07_09640"
     CDS             3206..4492
                     /gene="lepB"
                     /locus_tag="PKI07_09640"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOC51352.1"
                     /translation="MGKTEKRKAAPSGNATPAAGAAGREHAPATMPGVLRWLFSRTVR
                     RAMALHKHVQKILNHQRDLLSAPAIEAVQAAMADCRRAAQAGSPATLKEKMENLEQVA
                     NKWLKPYPHAAWRENVEVLLVALAVAMGIRTFFLQPFKIPTGSMQPTLYGVTSVPDFS
                     RGRDPAAEEAARDSLVIPSGWQRVCEWFKGVSYIRLTAAADGPVQAVDRPFRILIFNI
                     WQKVVAGGKTFILWFPPDYGASSLEERSGLLQRARDFHKGEDIVKMRVAAGDHLFVDR
                     MTYNFRPPQRGEILVFETRGIDGLPQDQFYIKRLVATGGERVQLGEDRHLIINGNRLD
                     ASVPHFENVYSFNPKEPPGDSRYSGHVNAARLAPYFDGRPEGVLVAPNHYMVMGDNTM
                     NSLDSRAWGDFPATNVIGKSFFVYWPFTERFGCGNR"
     gene            complement(4622..6067)
                     /gene="glgA"
                     /locus_tag="PKI07_09645"
     CDS             complement(4622..6067)
                     /gene="glgA"
                     /locus_tag="PKI07_09645"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOC51353.1"
                     /translation="MRILHASSEVYPYSKTGGLADMVGALAKALARTGRQVGVVTPLY
                     AGIRERFPDLKPVELPLDFPLGQRRVAASLWSLNPSPELTLYFVDQADFYQRPGLYQA
                     DGVDYPDNAERFVFFSKVIAHLALHLPWQPEVLHLHDWQTSLAALLLHHHRRTAGQGK
                     GPKVCLTIHNLAYQGLFPAAQYGLTNLPAEYFNLAGVEFYGQLGCLKAGIVYADIITT
                     VSPRYAREITTEEMGCGLDGLLRQRNSSLVGILNGVDADEWNPQSDPHLKHAYSAADL
                     RGKEMNKLELQREFGLPIRADIPLFGSIGRLAEQKGVEILLAALEETLGAKMQFIVVG
                     SGAPTFEKAYRDLARRYPSQVGVQIGFNEGLSHRIEAGSDFFLMPSRFEPCGLNQMYG
                     LRYGAIPIVRATGGLDDTVIDIREDSAKANGIKFMEYSPAALVKSIHKALALFEEPHL
                     MQQFRHNGMTADFSWNRVVSEYIRIYEKIGT"
     gene            complement(6064..7365)
                     /gene="serS"
                     /locus_tag="PKI07_09650"
     CDS             complement(6064..7365)
                     /gene="serS"
                     /locus_tag="PKI07_09650"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOC51354.1"
                     /translation="MLDIKLIRDQPDFVRQRLAARGAGDERLIDDLLKLDEQWRRQVS
                     EVETLKAQRKRVSKEIGALMGQKKTEEAEARKRETSALGDRIAALDRGAAEAEAARNQ
                     LMLRLPNLPHATVPPGKEAADNPVIRVHGEKPSFAFPAKSHVELCEKLGLVDFERATK
                     LSGSGFVLYTNWGAKLERALIQFMLDLHTSAHGYTEVSPPYMVGPQCLEGVGQFPKFA
                     DQYYGVQEGLDAGTLGKLYLIPTAETPVANIHREEILKESALPLRYCAYTPCFRGEAG
                     AAGVGTRGMIRVHQFDKVELISIVKPENGYTEHEQMVAGAERVLQSLGLHYRVVLLCT
                     GDMGFASAKTYDLEVWAPGQGSYLEVSSVSNCEDFQARRMNLRFKTEAGDNRFPHVLN
                     GSGTALARLFVALVETCQQADGTLAVPAALQPYLKTERITT"
     gene            7583..8878
                     /locus_tag="PKI07_09655"
     CDS             7583..8878
                     /locus_tag="PKI07_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51355.1"
                     /translation="MASRIPPSERITLGIVGWGMMGPANTDAFLKMADCQVVAACDLD
                     QHHLQAALDKINGHYGNRDCTPYADYREMMARKDIDAVMLAVPDHWHALTAIEAARNR
                     KDIYGEKPLARTVAEQQAIVKAVQKNQRIWQTGSWQRSQSSFRKAAEIVRNGLIGKVT
                     RVEVGLPSKHHDFAGTAPALLAKFRGSPNDPEYLAKIVPGSPEWQIAVTSPPPELDYE
                     MWLGPSRVEPYIQARIHMNWRWNYNIGGGQLLDWVGHHCDIAHWGLGFDNAGPFEVEG
                     HGEFPPPDALWNTCTKYRITLKYPNDVTMIMAGGHADIRGGTKWIGTDGWVWVDRGGY
                     DASNLDWFAQIPPDRDKVRLYRSDNHQRNFIDCVKSRQPTITPVETAHHSALPGHLGL
                     ISMLVGRKIKWDPVRERIQGDPGASKLLTRPYRPPWKLG"
     gene            complement(8903..10777)
                     /locus_tag="PKI07_09660"
     CDS             complement(8903..10777)
                     /locus_tag="PKI07_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51356.1"
                     /translation="MIRLLCAAILLAATLAAPAQWRVLSPSDQRQTQPPQNYAEGQEV
                     KLGEVCPEDQKFRWLVAELEIPATINAEATAGKVVGLQISCGDGGEVYVNGVLQCRYD
                     NDHPALAVLSESAVPGARVRVAVQVYAHVQGGNKFEQANWVIIDARRARERRVLSVDG
                     GHPAGKVPDGIVGLSQGGGMADYEAATARKLKEGGFKWFRMDNIFTGVLKAGQGTNLV
                     YDWTDFDRRVDFIAAIGADPILAVSYMPQVLDAVTNHERQSAPRDYAAWEELCFRAAQ
                     HSAQRGKRIPFWEVWNEVNAGWLKPGSADTGSETFRQLYTSALGKEEPNAEVVRRFEA
                     YCKLYRATARGVLRADPQAKVGGPALASGPFENSEYGHCQHGRGFARGLMLWCRQEKL
                     PLDFVSWHEYFQPPETIVKEAEAFRAYLADLPELKCSVTSFMITEWNEAWWADRPQDH
                     ELGAAWCANSITRAFLPARIDRPCFFYVKQNDMSFRGDYSLLMKDNLPKAAYNVAKIF
                     NSLRGDWLPVAGADDDVSAVAAWDAPAGRLAVVLVNFRDRYALRRPVQLRVNSLPEAL
                     KDGRWQESVVDATHSNAWHDPAKAELAVVRTGKISGLHLAWEQTLQPNSVTLLELLRP
                     "
     gene            complement(10958..13027)
                     /locus_tag="PKI07_09665"
     CDS             complement(10958..13027)
                     /locus_tag="PKI07_09665"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC51357.1"
                     /translation="MFQPVPFREFVEADPSCLAPPPAPAPARRVRTGFAMPGIRLPLF
                     ALLLLGGVAAAVAKTAPPPAAAPVKQLLAVLASGADETEKAAACRELGRLGARDAIAP
                     LAALLPNERLSHMARYALETIPDPAVEKALRDAAGQLQGHLRVGVIGSIGVRRDVKAV
                     RLLTRLLRDPDSEVAQAAARSLGRIGNATAAQALLDALPKAPAGRQPTICDGLLRCAE
                     EQSALGNHKRAIRIYDRLRTASITPPVRAAALRGAILARKQAGWPLLKASIRAGDLDL
                     FLVAIAAAGEMPGAEVTHILTDALAGQGADRQVLLIQALARRGDAAALPALCEAARSG
                     DTTVRVAALRTAAEFGRAAALPVFLDLMGDSDPDIAAAAQDGLARLPGREADDAIIRL
                     LTDGPSAQRGAALDLIARRRMASAVPALFAAATGAEPELRLAAIRKLGELAEPSELPR
                     LLDLLAGAANPAEVEAAEQAIRAVSLKAPASADCVSQVEARLAQASPPRKCALLRVLG
                     ALGGPRALSALRGALSDPNPEIRATAIRVLGDWGTADAAPDLLALARSATTATDQMIC
                     LRGCLRLAGLPDLPVDQRLAMCREAAALASNDEGKKLLLAALGTLTSVEAVDLALPYL
                     DEPGTREEASSAVVEMAARLLKGQNAARLAARLLGPLDKAGQATLNDDLAKRARALLD
                     QAKAKAG"
     gene            complement(13104..14108)
                     /locus_tag="PKI07_09670"
     CDS             complement(13104..14108)
                     /locus_tag="PKI07_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOC51358.1"
                     /translation="MRYAMRLAVALLAGAASWGVGKEITLLNVSYDPTRELYLEFNRA
                     FARHWQEQTGEGVVIRQSHGGSGKQARAVIDGLAADVVTLALAYDVDALWEKATLLPR
                     DWQKRLPHNSAPYTSTIVFTVRKGNPKQIRDWDDLARPGVSVIMPNPKTSGGARWNYL
                     AAWGYALKKWGGNEAKAREFVARIFKNVPILETGARGAMTAFAQRNLGDVLVAWENEA
                     LLLLKEPSPGGAELVIPSLSILAEPPVAWVDRVVNRRGTRVVAQAYLEYLYSPEGQEI
                     VAKHGYRPRLESAARKHAGTFPKVNLFTIDEVFGGWQKAQKTHFGAGGVFEQIQQAIR
                     "
     gene            complement(14169..15203)
                     /locus_tag="PKI07_09675"
     CDS             complement(14169..15203)
                     /locus_tag="PKI07_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004941759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC51359.1"
                     /translation="MAGTAYDRLQTELRAAPRTWLITGVAGFIGSNLLEALLKLDQRV
                     AGLDNFSTGKRKNLAEVKGLVSARQWAGFRLVEGDIGDLEICREACAGVDFVLHEAAL
                     GSVPLSMNDPLGAHRANVTGFLNMLVAARDAKARRFVYASSSAIYGNDRAVPITEERI
                     GKPLSPYAATKAINELYAQTFAQAYGFASIGLRYFNVFGPRQDPTGAYAAVIPQWITA
                     MLRQAPVYINGDGETSRDFCFIANVVQANLLAATTGHAEAVNEAYNVALGERTTLNEL
                     FHSLQLTLRAKDAALPMQQPVYREFRAGDVRHSLADISKAQRLLGYAPTHDIEGGLAL
                     AMDWYRANLT"
     gene            complement(15240..16304)
                     /locus_tag="PKI07_09680"
     CDS             complement(15240..16304)
                     /locus_tag="PKI07_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOC51360.1"
                     /translation="MSDVAILGAGLMGTAVAWPLSDNGHQVRLVGTHLDNEIIASCLE
                     KGWHPRLRRQLPNNVRPYYVEQLAEALEGVNVIVSGVSSPGVRWIGRTLAPHVKPGMK
                     IIAVTKGLEASAEGHLRILPDVLADELPPAVRGQVSLAAIGGPCIAGELAGRRQTCVY
                     FTSRDAAALPVLRQLFKTPYYHISLSTDVVGVEVCVALKNAYTLAVGLAGGMLDRLGG
                     PDAADAWNHNPAAALFGASAREMARLVSLLGGNPAMVTSLPGVGDQYVTCMGGRTVRL
                     GRLLGKGMSYEEARAEMAGETLESAFVVAQIARALPGLEARGLLGRDELPLMRCLCRV
                     ISGNAPVEVDFGRLFPDDVK"
     gene            17118..>17337
                     /locus_tag="PKI07_09685"
     CDS             17118..>17337
                     /locus_tag="PKI07_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51361.1"
                     /translation="MKILTSPRTGSLGAETYYQSPFGVCARRRTVPRDRPSNRKAAAR
                     SYFGISSREWGLKLTEAQRERWNAAALHV"
BASE COUNT         3388 a         5619 c         5222 g         3108 t
ORIGIN      
        1 gtaatagaca taagctccga ggcgtccgga gcgcggggaa tcgagtaatt tcataggtgt
       61 gagatttccg tggttatttt aatccaaggc cggaagggcg gcccggcggc tcgtgcctta
      121 cgaacctgtc ggccgggttg ccatgctttc acggcctggg gaaaaaggtt atgcccccgc
      181 cggataatcg caagcgccaa cttgcaaaca cacccggcgc cgcccatacg taaagacttt
      241 ttgcgcggag cgcataaatg agctagaata aaggccaaat gatacgccgg cttatacgca
      301 tcaccgcctt gctggtcctc gtggcggccc tggccgcctg ggtggcggcg ggagctaatc
      361 gcgggtggac gaaaacgtcc gttcccgtcc agcggacaga tgaagtcacc ggcctgacgg
      421 tggatgatta tcagaagcgc tttgttccgg gcgttgattt tctcgtggcc gccgtgttgg
      481 gggcgggcgt tttggccgcg agttcgtttc tttttcgcaa accgcaacca caaccatcaa
      541 actgaattag ccatcattcc attaccgatg aaacttctct cccgttatct tttgggcgcc
      601 ggactcctcg tcaacctcac cgtgctggcc gcgccgctga attttgactt caaggacccg
      661 aagggggtca ataatgcgac cttcaagctg gatgcgccgt tggaggccat cagcggcaac
      721 gccacgggca tatccggcac cgtcgcgttt gatccgcagg cgccggcggc cacaacgggc
      781 aagattgtcg tggcgacgtc ctccttgcgg gtgggcaacc cgattcagca ggagcacatg
      841 ctcggcgccc aatggctcga tgccgccaaa tacccggaga tctcctttac cgcccgggaa
      901 ctcaagaatg tgaagacgag cggcgacacc accaccgccg aggtggtcgg cacgttcagc
      961 ctcaagggca tttcgaaaac tctcaccgtg cccgtgaaac tgacgtatct gaaagacaag
     1021 ctggggcagc gcgtcccgga cttgaaaggc gatctgctgg tgatccgcgc caatttcagc
     1081 atcaagcgca gcgatttcgg gatcatgccg gggcaggcgg aggagaaagt tgcggagacc
     1141 atcgagctga cgttgagcat tgccggcgcc gcgccgcgct gaggcgccct ccgtgcgcca
     1201 acccgccggg atgcgggcgg ggtgacttgc tgaagatccg ccccgcgggg agccattccc
     1261 ggcggggcag gggagtggtg aatttccttt cttttcagag ggggaagccc tattttgacc
     1321 gggcctgaac cagagggctg tcgtactgcg aaaggggccg cgtctccttg ccgggcggcg
     1381 tctgccgggg cggcgcgata gtttgtgaaa gatggatacg gcgcatatta gaaattttag
     1441 catcattgct catattgatc acgggaagac gacgctctcg gatcggctgc tgcaccgaac
     1501 gggcaccatc accacgcgcg agatggagga tcagttgctc gattccatgg atttggaaaa
     1561 ggagcgcggc atcacgatca aagcgcaccc ggtgacgatg ctttaccagg ccgggaacgg
     1621 ggagacctat gaactgaacc tgattgatac gcccgggcac gtggattttt cctacgaagt
     1681 ctcccggagt ttgagcgcct gcgagggggc gctgctgatc gtggatgccg cgcagggggt
     1741 cgaggcgcaa accgtggcca atgtgcacct ggccatgaag cagaacctcg ccattatccc
     1801 ggtcataaac aaaatagacc tgccgcacgc ggatgtcgcg caggcgaagc ggcaactgga
     1861 ggatatcctg gcgattgccg gggattcggc cattttggcc agcgccaagg aaggcatcgg
     1921 tattgatcag atcctcgagg ccatcgtgga gcggatcccg gcgccgaaac cgaccggcgc
     1981 gccctccttg caggcgctgg ggtttgattg ctatttcgac acttaccggg gcgtggtcac
     2041 ccacgtgcgc gtgttcaatg gcgagctgaa gccgggaatg caggtcaagt tgctccattc
     2101 cggcaagaat gtggaggtca aggaagtggg cagctttaat ccgaagcctt acgtgcgcga
     2161 acggctcgaa tgcggcgaga ccggctatat gaccgccaat atcaagagcc cgctggaggt
     2221 caagatgggg gacaccatca ccgacgcgcg ccacccggcg cccgccctgc cgggattcaa
     2281 ggaaatccac ccgatggtgt tcagcgggat ttacccgatc aataccggcg actacgaaca
     2341 cctcaaggcc aacctggcca aactgcagtt gaacgactcg gcgtttgtgt accagcccga
     2401 gacttccgtg gcgctggggt ttggcttccg ctgcggcttc ctcgggctgc tgcacctgga
     2461 aatcgtgcag gagcggttgc gccgggagta caatatggac atcatcgcca cgtacccgag
     2521 cgtcatttac cgggtgaagc tcaccgacgg cacggaggtg cagattgaca atcccgccta
     2581 cctgccgccg gtcgcccaga tcgagagcat ttccgagccg atggtgcggg cgtttgtcat
     2641 ttgcccgaat gaatacatcg gggacatgat ggcgttaatc acggaaaaac gcggcgcggt
     2701 ggaccatacg gagaccctgg actcgcgtcg ggtgatgctc accagcctgc tgcccctcaa
     2761 cgagatcctg attgattttc acgaccgcat taaaagcatc acccgcggct atggctcgat
     2821 ggactacgaa ccggcggatt accaggcgtc caacatggtc aaattggacc tgctggtgaa
     2881 cggcgaggcg gtggacgcgt tttcatgcat cgtgcatcgg gacaaggcgg agggcagggg
     2941 acgcgcgctg gccgcgcggc tgaaggaagt cattccgcgc cagttgtatg ccgtggccat
     3001 ccaggccgcc atcggcggca aaatcatcgc ccgcgaaacc gtgagcgcat tgcgcaagga
     3061 cgtcaccgcc aaatgttatg gcggcgatat cacgcgcaag cgcaagctcc tggaaaagca
     3121 aaaggaaggc aaaaagcgca tgaaatcctt tggctcggtg aacatccccc aggaagcgtt
     3181 tatcgaagtg ctgaaggcct gacgtatggg caaaaccgaa aaacgaaaag ccgcgccgtc
     3241 cgggaatgca actcccgcag ccggggccgc gggacgggag cacgcgcccg ccaccatgcc
     3301 gggagtgctc cgctggttat tctcccgaac ggtgcggcgg gcgatggccc tgcacaaaca
     3361 cgtgcagaaa atcctcaacc accagcgcga tctgctctcg gcgccggcca tcgaggctgt
     3421 ccaggctgcc atggcggact gccggcgggc agcgcaagcg ggcagtccgg ccacgctgaa
     3481 agaaaagatg gagaacctcg agcaggttgc caacaagtgg ctcaaaccct atccgcatgc
     3541 ggcctggcgt gagaacgtcg aggttctgct ggtcgcactg gcggtcgcca tgggcattcg
     3601 cacgttcttc ctgcagccgt tcaagatccc caccggctca atgcaaccca cgctctatgg
     3661 cgtcacttcc gtgccggact tcagccgcgg ccgggatccc gccgccgagg aagcagcccg
     3721 ggacagcctg gtgattcctt ccggctggca gcgggtgtgc gaatggttca agggcgtgtc
     3781 ctatatccgc ctgacggcgg cggcggatgg cccggtgcag gcggttgacc gcccattccg
     3841 catcctgatc ttcaatatct ggcaaaaggt cgtggctggc ggcaaaacgt tcattctctg
     3901 gttcccgccc gattacggtg cttcatcgct tgaagaacga tccggattgc tccagcgggc
     3961 gcgggatttt cacaagggtg aagacatcgt gaaaatgcgg gtcgccgctg gagaccatct
     4021 gttcgtggac cggatgacct acaatttccg cccgccccag cggggcgaga ttctcgtgtt
     4081 cgagacccgg ggtattgatg gcctgccgca ggaccagttc tatattaagc gcctggtggc
     4141 gacgggtggc gagcgagtgc agctcgggga agatcggcac ctgattatca atggcaaccg
     4201 cctggatgct tcggtgccac actttgagaa tgtctattcc tttaacccga aagagccccc
     4261 gggcgacagc cggtattcag gccacgtgaa cgcggcgcgc ctggcgccat actttgacgg
     4321 ccggccggaa ggggttttgg tggcaccgaa tcattatatg gttatgggag acaacaccat
     4381 gaacagcctg gattcccggg cctgggggga tttcccggcc accaatgtca tcggcaagtc
     4441 gttcttcgta tattggccat ttaccgagcg atttggttgt ggaaaccgct aaaacacgcc
     4501 ataaaaaagc cagcctgcac gggattgtga tatacgcaca atgtttgtta tgtttaattc
     4561 ggatgagggt taatttgcct tggtagagcg atttttgggg ttggctaccc tgcccgctgg
     4621 tttaagtgcc gattttctcg tagatgcgta tgtattccga gactacgcgg ttccaggaaa
     4681 agtccgcggt cattccattg tgccggaatt gctgcatcaa atggggttct tcgaacagtg
     4741 ccagggcttt gtggatgctt ttaaccagtg ccgccgggga atattccatg aacttgatgc
     4801 cattggcctt tgcggagtct tcccggatgt caataaccgt gtcgtccagg ccgcccgtag
     4861 ctcggacgat gggaatggcg ccgtaccgga ggccgtacat ctggttaagt ccgcacggtt
     4921 caaaccggga cggcatcagg aaaaaatcgc tgcccgcttc aatgcggtgc gagagcccct
     4981 cgttaaaccc gatttgtacc cccacctggg agggataacg gcgtgccaaa tcccggtacg
     5041 ccttctcgaa cgttggcgct ccgctgccga ccacgataaa ctgcatcttg gcgccgaggg
     5101 tctcctccag cgccgcgagc aggatttcca cgcctttttg ttcggccaag cggccaatgg
     5161 agccaaacag cggaatgtcc gcgcgaatcg gcaagccaaa ctcccgctgc aattccagct
     5221 tgttcatctc tttgccgcgc agatcagccg ccgaataggc atgctttaaa tggggatcgc
     5281 tctgggggtt ccattcgtcc gcatcaacgc catttaggat gccaaccagc gaactgttgc
     5341 gctgccgcag caacccatcc agaccgcacc ccatctcttc ggtagtgatt tcacgcgcat
     5401 agcgcgggct cacggtggtg atgatatcgg catatacgat accggctttc aggcacccca
     5461 actgaccata gaactccacg ccggcgaggt tgaaatattc cgcggggagg ttggtgagcc
     5521 cgtattgggc ggccggaaag agtccctgat aggccaggtt gtgaatggtg aggcaaacct
     5581 tggggccctt cccctgcccg gccgttcggc ggtgatggtg aaggagcaac gcggccaggc
     5641 tggtttgcca gtcgtgcagg tgcagcactt ccggctgcca cggcaaatgc agggccaggt
     5701 gcgcgatgac tttggagaag aaaacaaaac gctccgcatt gtcgggataa tccactccgt
     5761 cagcttggta cagccccggc cgctgataga aatccgcctg gtccacgaaa tacagggtga
     5821 gttccgggct ggggttgagg ctccagagcg aagcggccac acgccgttgg cccaggggga
     5881 aatccagcgg caactccact ggtttgaggt cgggaaagcg ttcgcgaatc ccggcgtaga
     5941 gcggcgtcac tactcccact tgccggccgg tccgcgccag cgccttggcc agggcgccaa
     6001 ccatgtccgc cagcccgccg gtttttgagt agggatagac ttcgctgctg gcgtgcagga
     6061 ttctcatgtc gtaatccgct cggtcttgag ataaggctgc agcgccgccg gcacggccag
     6121 ggttccatcg gcctgctggc aggtttccac cagagccaca aacagccggg ccagcgccgt
     6181 gccgctgccg ttcaagacat gcgggaaccg gttgtcaccc gcttcggtct tgaagcgcag
     6241 attcatccgc cgcgcctgaa agtcttcgca attcgacacg ctggaaacct ccagatagct
     6301 cccctgcccc ggggcccaca cttcgaggtc gtacgtcttg gcgctggcga agcccatgtc
     6361 gcccgtgcac aacagcacca cgcgataatg caaccccagc gattgcagca cccgctctgc
     6421 gcccgccacc atctgttcgt gctcggtata gccgttttcg ggttttacga tgctgatcag
     6481 ttccaccttg tcgaactgat gcacgcggat catgccgcgg gtgcccaccc ccgcggcgcc
     6541 ggcctcgccg cgaaagcatg gcgtataggc gcagtagcgg aggggcagcg cgctttcttt
     6601 cagaatctcc tcccggtgaa tatttgccac cggggtttcg gcggtcggaa tgaggtaaag
     6661 tttgcccagc gtgccggcat ccaggccttc ctgcacgccg taatactggt cggcaaactt
     6721 cggaaactgc cctacccctt ccaggcactg cggccccacc atgtagggcg gcgatacttc
     6781 ggtgtagcca tgggcggagg tgtgcaagtc cagcatgaat tggatcagcg cccgttccag
     6841 tttcgcgccc cagttcgtgt acaatacaaa gccgctgccg gataacttcg ttgcccgctc
     6901 gaaatccacc aatcccagct tctcgcacag ttcaacatgc gacttcgccg gaaaggcaaa
     6961 ggagggcttt tcgccatgca cccgtatcac gggattatct gccgcttcct ttcccggcgg
     7021 caccgtcgcg tgcggcaggt tgggcagccg cagcatcaat tgattgcgcg ccgcttccgc
     7081 ttccgccgcc ccccggtcca gcgccgcaat ccggtcaccc aacgcgctgg tttcccgctt
     7141 tcgcgcttcc gcttcctccg ttttcttttg ccccatcagc gcgccgattt ccttcgacac
     7201 gcgcttgcgc tgggctttga gcgtttcgac ctcgctcact tgccggcgcc actgctcgtc
     7261 cagcttcagc aaatcatcaa tgaggcgctc atcgcccgcg ccccgcgctg ccaggcgctg
     7321 gcgcacaaag tcgggctggt cacgtatcaa tttgatgtcg agcatcggcg cattataggc
     7381 gcgcgcgcgg ctccggcaag aatgaaggga cggcgggccg ccgtgcgctc ctttttgttc
     7441 ttgtctttgc gcgcaacttg tggcgccatg cgcttccatg aacgaaactt cctccgctgc
     7501 cggaatcagc cgccgccgct ttctggcggc aaccggattg gtccttgccg cgcctcccct
     7561 gttctcttcc tgcgctacgc gaatggccag ccggatcccg ccctctgaac gcatcaccct
     7621 gggcatcgtc ggatggggaa tgatgggccc cgccaacacc gacgccttcc tgaaaatggc
     7681 ggattgccag gtggtggccg cctgcgacct tgaccagcat catctccagg cggcgctgga
     7741 taagatcaac ggccattacg gaaaccggga ctgcaccccc tacgctgatt accgggaaat
     7801 gatggcccgc aaagacattg acgcggttat gctggccgtg ccggatcatt ggcacgcgct
     7861 cacggccatc gaagccgccc ggaatcggaa agatatttat ggcgaaaagc cgctggcccg
     7921 cacggttgcc gaacagcagg ccatcgtcaa ggcggtccag aaaaaccagc gcatctggca
     7981 gacgggttcg tggcagcgat cgcaaagctc attccgcaag gcggcggaaa tcgtgcgcaa
     8041 cggcctcatc ggaaaagtca cccgcgtgga agtcggcctg ccgagcaagc accatgattt
     8101 tgccgggacg gccccggctt tgctggcgaa gttccggggt tcaccgaatg atcccgaata
     8161 cctggcaaag atcgttccag gcagcccgga gtggcagatc gcggtgactt cgcccccgcc
     8221 ggagctggat tatgaaatgt ggctcgggcc ttcccgggtg gagccttaca tccaggcgcg
     8281 catccacatg aactggcgct ggaactacaa tattgggggc ggtcagttgc tggactgggt
     8341 ggggcatcat tgcgacatcg cccactgggg gcttggtttc gataacgccg ggccgtttga
     8401 ggtcgaaggc cacggcgaat tcccgcctcc ggacgccctt tggaacacct gcaccaaata
     8461 tcgcattacg ctcaagtatc cgaacgacgt cacgatgatt atggccggcg gccatgctga
     8521 tattcgcggg ggcaccaaat ggatcggtac cgacggctgg gtctgggtgg atcgcggtgg
     8581 atatgacgct tccaatctcg actggttcgc ccaaattccc cccgatcgcg acaaggtgcg
     8641 gctctaccgg tcggacaatc accagcgcaa cttcattgat tgcgtgaagt cacgccagcc
     8701 caccattact cccgtcgaga ccgcgcatca ctccgccctg cccggccatc tggggctcat
     8761 ctccatgctc gtggggcgca agattaagtg ggacccagtc cgggagcgca tccagggaga
     8821 cccgggggcc agcaaacttc ttacccgccc ttatcgcccg ccctggaagc tgggctaggc
     8881 agccgcccgc gacaagggcg gttcacggcc ggagcaactc caacagggtg acggagttgg
     8941 gctgaagggt ttgctcccag gcaaggtgca ggcctgagat tttgcccgtg cggacaaccg
     9001 ccaactcagc tttagccggg tcatgccagg cattgctgtg ggtggcatcc accacggact
     9061 cctgccaacg cccgtccttc agggcttccg gaagcgagtt gacgcggagc tgaacaggcc
     9121 tgcgtagagc atagcggtcg cggaagttga cgaggacaac cgccaggcga cccgccgggg
     9181 catcccaggc ggcgacggcg gaaacgtcgt cgtcagcacc cgccactggc agccagtcgc
     9241 cacgcaggct gttgaatatc tttgccacgt tgtaggcggc tttggggagg ttatccttca
     9301 tcagcaggct gtagtcgccg cggaagctca tatcattttg cttcacgtag aaaaagcagg
     9361 gccggtctat gcgcgcgggc agaaaggccc gggtaatgga attggcgcac caggcggccc
     9421 ccagttcgtg gtcttgtggg cggtctgccc accaggcctc gttccattcg gtaatcatga
     9481 aggaagtcac agagcactta agctcgggca ggtcggccag ataggcgcga aaggcctcgg
     9541 cttccttgac gatggtttcg ggcggctgga agtattcatg ccagctcaca aagtccagcg
     9601 gcagcttttc ctgccggcac cagagcatga ggccgcgcgc aaaacccctg ccgtgctggc
     9661 aatggccgta ttcggagttc tcgaaggggc cgctggccag ggcggggccg ccaaccttcg
     9721 cctgcggatc ggcgcgcagc accccgcgcg cggtggcgcg atagagtttg cagtaagcct
     9781 cgaaccgccg gacaacctcc gcattcggct cctccttgcc gagcgcggac gtgtaaagct
     9841 gccggaaagt ttcactgccc gtgtccgcgg agccgggctt gagccagccg gcattgactt
     9901 cgttccaaac ctcccagaaa gggatgcgtt tgccccgctg cgcgctgtgc tgggccgcgc
     9961 ggaagcaaag ctcttcccag gcggcgtagt ctcggggcgc gctttggcgt tcgtggttgg
    10021 tcacggcgtc aagaacttgc ggcatgtagg ataccgccag aatggggtcc gccccgatgg
    10081 cggcaatgaa atccacgcgc cggtcgaaat ccgtccaatc ataaaccagg ttggtcccct
    10141 gccctgcttt caggacgccg gtgaaaatgt tgtccatgcg gaaccatttg aagccgcctt
    10201 ccttgagctt gcgcgcggtg gcggcttcat aatcagccat tccgcccccc tggctcaggc
    10261 cgacgatgcc atcaggcact ttgcccgccg gatgcccgcc atccaccgaa aggacccggc
    10321 gctcccttgc ccgccgggca tcaatgataa cccagttagc ctgctcgaac ttgtttccac
    10381 cctgcacatg ggcatagacc tggacggcca cccgcacccg ggcgccgggc accgccgact
    10441 cgctcaagac agccagggcg ggatgatcat tatcataacg gcattgcagg acgccgttga
    10501 catacacctc gcctccatcc ccgcagctaa tctggaggcc gaccactttg cccgcggtgg
    10561 cctccgcgtt gatggtggca ggaatctcca gttcggccac cagccagcgg aacttctgat
    10621 cctccgggca aacctcgccg agcttgacct cttgaccctc agcatagttt tggggcggct
    10681 gggtttggcg ttggtccgat ggcgaaagga cgcgccactg agccggcgcg gcaagcgtgg
    10741 cggccagaag aatagcagcg cagagtaagc gaatcatggt tatcgcggtt caggcaaaaa
    10801 tagagcatag tcccggcagc ggcgcgaatc atttatggca aatcttaccc cgatagccgg
    10861 aactgctgtt cttaaaagcg tgggcaggga gagtgtctgg ccaggggttt ccaactgccg
    10921 ggagttaagc gcgttaacag cactcaggaa gggcatttca tccagccttg gctttggcct
    10981 ggtcgagcag agcccgggcg cgcttggcca ggtcgtcgtt gagggtggct tgtcccgcct
    11041 tgtccagggg gccgagcagg cgcgcggcca gccgggctgc gttttggcct ttcaacagcc
    11101 tggcggccat ctccaccacc gccgagctgg cttcttcccg ggtgccgggc tcgtccaggt
    11161 acggcagggc caagtccacc gcctcgacgg aggtgagcgt gcccagggcg gccagcagaa
    11221 gtttctttcc ttcgtcattg gaggccaggg ccgccgcctc gcggcacatg gccaggcgtt
    11281 gatccaccgg caggtcgggc agcccggcca ggcgcaggca gccccgcagg cagatcatct
    11341 ggtcagttgc ggtggtggcg cttcgggcca gcgcgagcaa gtcgggcgcg gcatcggcgg
    11401 tgccccaatc gccgagcacg cggatagccg tggcgcggat ttcgggattg ggatcgctaa
    11461 gggcgccgcg cagcgctgaa agggcccgag gaccgccgag cgcgccgagc acgcggagca
    11521 acgcacactt ccgaggcggc gaagcctgcg ccaggcgggc ttccacctgg ctgacacaat
    11581 ccgccgaggc gggagctttc aaggagacgg cccgaatggc ctgctcggca gcttccactt
    11641 ccgccggatt tgccgccccc gccagcaaat cgagcaaccg tggcaattcg gaaggttcgg
    11701 ccagttcgcc gagcttccgg atggcggcga gccgcaactc cggctccgct ccggtggcgg
    11761 cggcaaacag cgcggggacg gccgaggcca tgcggcggcg cgcaatcaga tccaatgcgg
    11821 cgccgcgctg agctgacgga ccatcagtca gcagccggat aatagcgtca tccgcctccc
    11881 gccccggcag ccgggcaagc ccgtcctggg cggcggctgc gatgtctgga tcggagtctc
    11941 ccatgagatc gagaaacacc ggcaacgccg cggcacgccc aaactccgcc gcggtgcgca
    12001 gagccgcaac ccggaccgtc gtatccccgc tgcgcgcggc ctcgcagaga gcgggcaggg
    12061 ccgcggcatc gccccgtcgg gcgagggcct ggatcaggag gacttgccgg tcggcgccct
    12121 gccctgccag cgcgtcggtt aggatgtggg tgacttccgc gccgggcatt tcaccggcag
    12181 cggcgattgc gacgagaaag aggtccaagt cgccggcccg gatagatgcc ttcagcagcg
    12241 gccagcctgc ttgctttcgc gcgagaatcg cgccgcgcaa ggcggcggcc cgcacaggag
    12301 gcgtaattga ggccgtgcgc aagcggtcgt agatccgaat ggcgcgcttg tgattgccaa
    12361 gggcgctttg ctcctcagcg caacgcaaca ggccgtcgca aatggtcggc tggcggccgg
    12421 ccggggcctt gggcagggcg tccaggagcg cctgggcggc ggtcgcgttg ccgatgcggc
    12481 ccagggacct ggccgccgcc tgggcaactt cggaatcggg atcccgcagc agccgcgtca
    12541 ggagccgaac ggccttgaca tcgcggcgga cgccgatgct gccgatgacg ccgacccgca
    12601 ggtggccctg gagctggcca gccgcgtcgc gcagagcctt ctccacggcg ggatcgggaa
    12661 tggtttccag ggcatagcgg gccatgtggg aaagccgctc gttgggcagc agtgcggcca
    12721 gcggggcgat ggcgtcccgg gccccgagcc ggcccagttc gcggcacgca gcagcctttt
    12781 ccgtttcatc agccccggaa gccagcacgg cgagcagttg cttgacgggg gcggccgcag
    12841 ggggcggggc ggtttttgcg actgcggcgg cgaccccgcc cagcagcagc agggcgaata
    12901 aaggaaggcg aataccgggc atggcaaagc cagttcgaac gcggcgggcg ggcgcgggcg
    12961 ccgggggcgg agccaagcag gagggatcgg cctcgacgaa ctcccggaag ggcactggtt
    13021 gaaacattaa ccaagtttag ccgggccgtt tcggctccgc aagcctgccg ccccggggcg
    13081 ttcccctgcc cggccccggc ggcctaccgg atggcctgct ggatttgctc aaacactccg
    13141 cccgccccga agtgggtttt ctgcgccttt tgccagccgc caaaaacctc atcaatggtg
    13201 aacaggttga ccttgggaaa ggtgccggca tgctttcgcg cggcggattc caggcgcggc
    13261 cgataaccgt gcttcgccac gatttcctgg ccttccgggg aatacaggta ctcgagataa
    13321 gcctgggcca ccacgcgcgt cccgcgccgg ttgaccaccc gatccaccca cgccaccggc
    13381 ggttccgcca ggatgcttag cgacggaata accagctcgg cgccccccgg actgggctct
    13441 ttcaacaaga gcagcgcctc attttcccag gccaccagaa cgtcgccaag atttcgctgg
    13501 gcgaatgcgg tcatggcgcc gcgcgccccc gtctcaagaa ttggcacgtt cttgaatatc
    13561 cgggccacaa attcccgggc tttcgcctcg ttgccgcccc acttcttcag cgcatagccc
    13621 caggccgcca ggtaattcca gcgcgcaccc ccggaagtct tcgggttggg catgataacc
    13681 gatacccccg gccgggccag gtcatcccag tctcggatct gcttcggatt ccccttccgc
    13741 accgtgaaaa ctatcgtcga ggtgtacgga gcgctgttat gcggcagccg cttctgccag
    13801 tcccgcggca gcaacgtcgc cttttcccaa agcgcatcca cgtcataagc cagtgccagc
    13861 gtcaccacat ccgccgccaa accatcaatc accgcccgcg cctgcttccc cgaaccgccg
    13921 tgcgactgcc ggatgaccac cccctccccg gtctgctcct gccaatgccg ggcaaacgcc
    13981 cggttgaact ccaagtacag ctcgcgggtc ggatcatacg aaacattgag caaggtaatc
    14041 tccttcccca ccccccaact cgccgccccc gccagcaaag cgacggccaa ccgcatcgcg
    14101 taccgcatgc ccatgccgcc aatgtcccct gcgccggctc aaaaagccag ccgaatattc
    14161 cccggccttc aggtgaggtt ggcccggtac caatccatcg ccagggcaag ccccccctca
    14221 atatcatggg tgggggcata gcccaacagc cgttgcgctt tgctgatatc cgccagcgaa
    14281 tgccgcacat cgcccgcccg aaactcccgg taaaccggct gctgcatcgg gagcgccgca
    14341 tccttcgccc gcaaggtaag ctgcagcgaa tggaaaagct cgttgagcgt cgttcgttcc
    14401 cccagcgcga cgttgtaagc ctcgttgacc gcctcggcgt ggccggtcgt ggccgccagc
    14461 aaattcgcct gcaccacatt cgcaataaaa caaaaatccc ggctcgtctc gccatcccca
    14521 ttgatataaa ccggcgcctg ccgcagcatg gcggtaatcc attgtgggat caccgccgcg
    14581 taagcgcccg tcgggtcttg ccgcggccca aacacgttga aatacctcaa cccaatggac
    14641 gcaaaaccat acgcctgcgc aaacgtctgc gcataaagct cgttaatcgc cttggtcgcc
    14701 gcatacggcg aaagcggctt gccaatccgc tcttccgtaa tcggcaccgc ccggtcattg
    14761 ccataaatcg cgctgctcga cgcataaacg aaacgccggg cctttgcgtc ccgcgccgcc
    14821 accagcatgt tcaaaaaccc cgtcacgttg gcgcgatgcg ccccgagcgg atcgttcatg
    14881 gacagcggca ccgaccccag cgccgcctcg tgcagaacaa aatccactcc cgcacaagcc
    14941 tcccggcaga tctccagatc cccgatatct ccctccacca ggcgaaatcc cgcccactgc
    15001 cgggcgctga ccaggccctt cacttccgcc aggttcttcc tcttcccggt ggaaaaattg
    15061 tccaaccccg ccacccgctg atccagcttc agcaaggctt ccagcagatt ggaaccaata
    15121 aagccggcca cgccggttat cagccacgtc cggggcgcgg ctcgcaattc cgtttgcagt
    15181 cggtcgtaag cggttccagc cattccgtgt cgcgttcgca ggtttattcg gatctcaaat
    15241 cacttcacgt cgtccggaaa aagacggcca aaatcaactt caaccggagc gttcccgctg
    15301 atcacgcggc aaaggcagcg catgagcggc aactcgtcgc gccccagcaa cccgcgcgcc
    15361 tccagcccgg gcagcgccct ggcaatctgc gccaccacaa acgcgctttc cagcgtctcc
    15421 ccggccatct ccgcgcgcgc ttcctcgtag ctcatcccct tgcccagcag ccgccctaaa
    15481 cgcaccgtgc gcccccccat gcacgtcaca tactgatccc ccacgccggg cagcgaagtg
    15541 accatggccg gattcccgcc cagcagcgac accaaccgcg ccatctcccg ggcgctggcg
    15601 ccaaaaagcg ccgcggcggg attatgattc caggcatccg ccgcatccgg cccccccaaa
    15661 cgatccagca tcccccccgc cagccccacc gccagcgtat aggcattctt cagcgccaca
    15721 cagacctcca cgcccacaac atccgtcgaa agcgaaatat gataatacgg cgtcttgaac
    15781 aactggcgca aaaccggcag cgccgcggca tcgcgcgacg tgaaatagac gcaggtctgc
    15841 cgccgcccgg ccaattcacc ggctatgcaa ggccccccaa tggccgccag gctcacctgt
    15901 ccccgcaccg cgggcggcaa ttcgtcagcc agaacgtccg gaagaatgcg cagatgcccc
    15961 tccgcagagg cctccaaccc cttggtaacg gcgatgatct tcatgcccgg cttcacatgc
    16021 ggggccaaag tgcgccctat ccagcgcacc ccgggcgaac tcaccccgct gacaatcaca
    16081 ttgaccccct ccagcgcctc ggcgagctgc tccacataat acggccgcac attgttgggc
    16141 agttgccgcc gcaaacgagg atgccagccc ttctccaaac aggaggcgat aatctcattg
    16201 tccaggtgag tgccgactaa acgaacctgg tggccgttat cgctcaaagg ccaggccacc
    16261 gccgtcccca tcaatcccgc gcctaaaatt gcgacatcag acatggtctg ctccgtttcg
    16321 actgaaaatc atggctaatc tcatgaccgg cgcattgtcc gcaaacggat gccgaaagtc
    16381 caaacagaat tgataaagtt aacgagaaac aagcaccacc ctggcaatcc tctctcacca
    16441 cactcgccaa acgcaccaac ccggaaccct gcaagggttc ccggagatag cccagggtca
    16501 gcgaggcacg agcgccaccc tgggtaaccc gtcccaaaat atcaatcccc cctctcctcc
    16561 gcctgcggag gagagggaac gggggagagg aggttctcgg gaccacccac acaagggacg
    16621 acgtgggtgc gagagcccgg gcatggaagc tcgtcaaata cgagtcgcgc ctgtcaagtg
    16681 cctgcccgaa cgtcaacctc gttatttacg gtggcttggc tacaggcaac ccaggcgagc
    16741 gcgtaaaggt ttttcacaac cgccagacaa gcggcccccg agcgccgcgt gagggcacgc
    16801 ggcctgcagg aaatgaaagc acgccgcggc agcccccatg ccctctcgca gcaccgcact
    16861 cccggcaaac cacaggcccc ctcgcatcat cccgccccct gtaggccgcg tgcccacacg
    16921 cggcgctttg caccggcctg ccacccccaa cccccaaaaa ttgcccttgc gcgaaggcac
    16981 cccgcccgcc agccttcccg cgtgggctcg gggtggcgac ggcggggact tccagcccaa
    17041 gaaatggcag gaagccgagt cgccctccgg ggcgatgaca gaacgacaat aaaaaaacaa
    17101 caaaaaggaa ctacgttatg aaaatactaa cctctccgcg cacgggcagc ctgggcgcgg
    17161 aaacatatta ccaatccccc ttcggcgtgt gcgcccggcg gcgcaccgtt ccccgggacc
    17221 ggccttctaa ccgcaaagcg gccgcacggt cgtattttgg gatttcctcg cgggagtggg
    17281 gcctcaagct caccgaagcg cagcgcgagc gttggaacgc cgcggcgctg catgttc
//
LOCUS       DAOPQG010000079        16936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4044_length_16936_cov_12.463480, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000079 DAOPQG010000000
VERSION     DAOPQG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 16936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16936
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4044_length_16936_cov_12.463480"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1535)
                     /locus_tag="PKI07_09690"
     CDS             complement(258..1535)
                     /locus_tag="PKI07_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51362.1"
                     /translation="MDNSIKIALVGTGMFGGDVHARAYADLQRAGLSPQLGRVGLDGW
                     ARDLAPIRFELAAVAARTETSARRARRNFQRWTGHAPRAFAGPKPWREILRAFPRLDV
                     MAVATPDPLHAEVILAALQAGVHVITEKPMCLDIAEADQIIALARRKNRIVAVDMHKR
                     YDPDHARIREEIQQRLGAPLYGSAYLEEPLRVATRAFKWVEQSDPFSYVGSHWVDLIY
                     HYYRSKPVSLTAVGQKRRLVRDGINAYDAVQVRVDFANGLSVTFHNNWITPPGFEGPV
                     NQGHEIVGTDGKVESDQQYRGLRWWSAGGGSRTANTHFTRNVKRPDASTAYVGYGIDS
                     LTAALAAVCRVKYFGARRASVASLYPTAEEARITVAIVHAARLVRNLNFKYLRTGKGA
                     PVTAHFGRRGITIVDPYRAGGRKGFQRVYSKPI"
     gene            1804..4632
                     /locus_tag="PKI07_09695"
     CDS             1804..4632
                     /locus_tag="PKI07_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOC51363.1"
                     /translation="MISAQQQATLSGANCEIAFDNLTRQLYATDASIYQVVPAAVAFP
                     ANIRQVVLLLDAAVMAGVTVTARGAGTGLAGGALGDGLVLDFSRYNRQITDLDLERRT
                     VRVGPGVVLDQLNNFLRPHKYWFGPDVATSSRATLGGMIANNSSGAHAPIYGTTADHV
                     QELEVVLADGRVTLVGAKHDTLRKQRERVSDLLYFHGLSIAEQRPPQLTKRWPGYAIE
                     RCAPEPNNLNPLLCGSEGTLAIIVAAELNIVPLPQQKGLGLIFFASVTEAMQATVELL
                     DLQPAAIEHLDRVLLDQTKGQPRFQAARDLLELDAKPCQSILAVEFFDDVEERLAALE
                     RRRLGLRQTLLKTEAHMKQIWALRQAGLSLLTGRKGEAKPVTGIEDAAVRPRDLPAYV
                     SGLQSLLAPLGLQASFYGHAAAGLLHVRPILNLGSAADAKKFRQLADEVSALVKRFKG
                     SLAAEHGVGMARAEYMPQQLGDGLLSVMREIKSAFDPNNLLNPGKIIPDGRFKVDASL
                     RRGPGYELKLPFTPLLAFAAKDGSFTRNLEQCNGCGGCRKETPTMCPTFLVTGEEIMS
                     TRGRANAIRAALERRGGAGGDVLRGAELEAALSNCLSCRACTLECPSNVNMSLLKAEL
                     LHARIQRDGLSWRERLISKVDLLGRLGCAWPRLANAALASNFVRRPLNRMLGLAWQRP
                     LPRYARLRFDRWFARRGASPQAPRGRVVLWDDTFVRYHEPHIGRAAVKVLEAAGFEVT
                     LPAGRKCCGRPAFSQGNLAEAQRLGRHNLALLSRDGDGAPIIFLEPSCYTMFAEDYRE
                     LKLDGAESVARRCFLFEPFIEGLLSHEPDALRFKTRAGHVAIHAHCHAKALLDPACLQ
                     RLAERLPERNVTLLETGCCGMAGAFGMMASKYELSLKVAEPLMQKVRSLPFGTLIVAS
                     GTSCRQQIDHLAPVRARHMAEVLADALA"
     gene            4748..6199
                     /locus_tag="PKI07_09700"
     CDS             4748..6199
                     /locus_tag="PKI07_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51364.1"
                     /translation="MKLSRMKPGQAVRRLLPVWVAMLGLLGAPEAGVGAGATAGWAEV
                     EITPPLGIALGGRAGPVTPADKVLDPLFAQVLCLRDGLGKGLVLVSLDLIGLPHDLSD
                     RLRAQLVQELGVGWNLVVLNASHTHSGPYMLRSLVAGLGPPPPVEAEYFDTLEAKVIG
                     AARAAGRALRPVQVEVFHGRTKLGINRRGINKEGRYAGMVPNENGPRDDRVWAMKLTP
                     TQGGAPALVFSCACHAVIVYGFDYAALSADFPGATRNLLRAALDPKAHLQFVQGFAGD
                     VRPRVLADLERGRFRKAIPADLEQAARELSGAVLSALKTPGEKLSLDLAGASDRPYLP
                     RGAPPPRERYETMRATALAQTNAHRLALAEYWLPRYDAGEGFARGNAWPLGLIRLAEN
                     QWIVHCAGEPCSAWRGLMAQWLAPLNLVAWGYSQEAQSYLPTESMLPEGGYEVVDCNL
                     GRKAGSAPLAPGIEAAVRQSLLRQLAFIRAGAR"
     gene            6301..7245
                     /locus_tag="PKI07_09705"
     CDS             6301..7245
                     /locus_tag="PKI07_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOC51365.1"
                     /translation="MPALAYNHGVSGQFRQPIDASTRFCAVYGYPVRHSASPAMQNAG
                     LAALGLNWRYLACEVRPEDLRAAIQGAQAMGFLGLNLTVPHKEMARDLVDALDASART
                     WGAVNTIRFEGRDEHGAWRALREFSAPPRQTRSQGFNTDADAIVRSLREDLGLELRGA
                     KVLLLGAGGAGRTAALKLAAEGVAELCLVNRTRGKAEAVATEIREHYPEVRLALGYPA
                     GPVELVLNATSLGLKAGDALPLDAGQFPFRRAGAAYDMIYRPAETAFLQAAQAAGCRV
                     ANGLGMLLYQGAAALELWTGQPAPLGNMRQALERNIYG"
     gene            7256..8365
                     /locus_tag="PKI07_09710"
     CDS             7256..8365
                     /locus_tag="PKI07_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOC51366.1"
                     /translation="MLTPNFDPNLWAAVPFHFWSVASVVLGSMVGSFLNVCIHRLPLE
                     QSVISPPSHCPHCNYSIPWYLNIPLFTWLYLRGKCRNCGAPISIRYFLVELLTAVTFL
                     CCWLTFGPCSAALALVYALFLSGLIAATFIDFEHFIIPDEITIGGMVAGFACSFFWPA
                     LQGQASPAGGLRQSLLGMGAGAGIIYLILRGGKLLFGRQRVTLPAHTRVVFGESGVHL
                     PDREIPYDELFYRRSDVITLYARTLELADRGYKDVRVRLAPDRLQIGEEELNPEEVPH
                     LEAVCTEIVFPREAMGLGDVKFMAAIGAFLGWPAVVFSLMVSSMIGAVIGVSLILLRK
                     REWSSHLPYGPYIALAATVWIFGGKRLVRWWLGLE"
     gene            complement(8417..9805)
                     /locus_tag="PKI07_09715"
     CDS             complement(8417..9805)
                     /locus_tag="PKI07_09715"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOC51367.1"
                     /translation="MNSLLLTGGRVIDPANDFDARAEVLICDGKIAAVGPAVAGQAPA
                     GVERLDVGGRVVCPGLIDMHVHLREPGQTAKETIASGTAAAARGGFTSILCMPNTSPA
                     TDNAGTVALIRERAAREGVVNVYVAGAITKGLTGEELAPIGSLKKAGVAAITDDGHCV
                     QNTELMRRAMEYAKMFELPVLDHCQDYSLVTEGVMREGYWSTALGLRGWPAAGEETIV
                     ARNILLAEMTGAQVHCQHISAAGSVRLLREAKRRGVPISGEACPHHFTLTDAAIAGSQ
                     KFLETDGPGVFGLGSGSRPRPAWPAYDTNFKVNPPLGTARDREAILEGLADGTLEILG
                     SDHAPHCDYEKEVEFDYAPFGITGLETALALALMQLYHSGRLGLAEVIAKFTLAPARR
                     LRLPHGTLSVGAVADVTVFDPDRAWVFEPAATASKARNSPFYGWPLLGKAVATIVGGR
                     KAWVETGALAAA"
     gene            complement(9909..10619)
                     /locus_tag="PKI07_09720"
     CDS             complement(9909..10619)
                     /locus_tag="PKI07_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC51368.1"
                     /translation="MSVTDCGLRAQARNHRRAPAPPPPDPRVAFFDQRAPTWDDDADD
                     VRRTLARLAELRGRIGLVPGQDVLEVGCGTGRITGWLVETVRPGRVVAVDFSPAMLAQ
                     AAVRRLAAEFRWLDVCGEIAAAEQFAVALCFHAFPHFRDQPRALRNLRRLLRPGGQLI
                     ILHLAGSAELNHFHSQLAAPVCHDLLPAAAAWPALLGRAGLELVSLVDTEGLFLLKAV
                     SPPTPARREAPRAAAARE"
     gene            10765..11592
                     /locus_tag="PKI07_09725"
     CDS             10765..11592
                     /locus_tag="PKI07_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOC51369.1"
                     /translation="MPKLTVLLLAGLLGAAGGCHRAAPAGRGPVIASATSYLESAARD
                     VLGDDFDVLRLAEPGTCPGHFDIRPSQAAALRRCRALLRFDFQSSLDRLLEPGGTNRP
                     LVVAVAVRGGLVQPASYLSACRQVAEAFVAANQLPRQRADLQLQRIAARLEALAQEAT
                     NRVAQAGLRGAPVIASVHQRDFCEWLGLRVVAAFRAADTASVAEIDEAIRAGKLAEAR
                     LVIANQPEGRRTADALAERLKARVAVLGNFPAGEHGRASFDNLLTGNVNALVRVSRP"
     gene            11589..12392
                     /locus_tag="PKI07_09730"
     CDS             11589..12392
                     /locus_tag="PKI07_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOC51370.1"
                     /translation="MSPPAVALEKLVVRAGRRRLLDVAELRVPAGEIVAVIGRNGAGK
                     TTLLRACLGLVKPAQGAVRVLGQEVWGRDAGARAQLRRRVGYVPQLLPARSEMPLTVR
                     EVVAIGRTGRAGLFHPLRPGDWQLVDEWIERLGLAALARRAFSEISGGEQRKTIIARA
                     MAQQPELLLLDEPTANLDLGWRERMVAAIQQLHAELAISILLVCHELEVLPPACRRVV
                     LLDGGRVWASGPPETIFTDERISALYGAGLRVAHEGGRHAVLPGSGAHV"
     gene            12358..13197
                     /locus_tag="PKI07_09735"
     CDS             12358..13197
                     /locus_tag="PKI07_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOC51371.1"
                     /translation="MPSCRGAVRMFDFVFWLPVMAGGALAGASSGLLGTFIVGMRIPF
                     LGVCVAHAALAGAVFGSLGGLAGQALLLPALGASVLTALALGLLDPQRVRLDDNAVLS
                     FLFSATMGLAFLGIGLHGILGRSDNDVRSMLWGSLNFCRWRDVGLMLASSGAVLAYIV
                     VFFKELRAIMFSRADAEAAGIRVKVVWSGFLVLVAAVLTVNFQTVGGLMIYSLISNPA
                     AAAFQLVKSCRRTLAVSAVLGAASGLGGFLISAATDLPSGAVIVLVSSVLLALAAIVR
                     RCR"
     gene            complement(13271..15028)
                     /locus_tag="PKI07_09740"
     CDS             complement(13271..15028)
                     /locus_tag="PKI07_09740"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC51372.1"
                     /translation="MNPNWLIVPPLGVDGGVYSIEVAHTTLTTSPDVVVTAYCADNFY
                     GQTSAGCTNSAVFNQANGGNTWKLMGYITNAPGITDPEIIFKQVGGVTGPGSNEKRLY
                     VDAFKFTLIDACAGVVGDLSVNGPLAAGQMFVTVSGVTAGATNVTIFANDAEIGQTNL
                     AAGFAAGALSVPVNTGLVKGNIIKATQKKPSAAPEGYCVSPLPADGPLVGSGANPALK
                     VVLSCGAQSALTGPAGADTSGAATTLYHVNATGLAGGSATAPVGGVALVPGSCWQTVT
                     FDFATDPMLNWMAGTAYSDSDPFCTFDALSFAIDPDHPDTGPYDIYVDKIMNGDVVIE
                     DFEGWEAGDSVMFAAPNATGVPDPAIHVLGGGTTSSVISQANAYEGTNSCRIRWQFKN
                     EGQTRWVRVLANRSNGATYPQLDKSKPVTLRLLVLPVGETTAKLGVGPVASQTKAPGQ
                     SVTFSVVATGVGPFSYQWKHNGSNVGTDSSSYTIGSVSAGDAGNYTVVVSNAACGSIE
                     SSPGVLTVSAPAVEPVKIDSITATTIAYSTGAGTQFILLKAAAVNAPMNTWVPVATNN
                     ATPGTFAVPGPGFYRIESK"
     gene            complement(15508..16794)
                     /locus_tag="PKI07_09745"
     CDS             complement(15508..16794)
                     /locus_tag="PKI07_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOC51373.1"
                     /translation="MATLTEKTPPSQRLMSVDALRGFDMFWIIGADSLVYALNRMSPT
                     PPTRFLARQLEHAEWAGFRFYDLIFPLFIFIVGVSLVFSLSRTIALEGRARAIQRIVR
                     RSLLLFVIGIFYSGGLTNPWPDLRLMGVLNRIALAYFFAGALFCFFRPRALAAICVGL
                     LAGYWALMTFVPVRDLPLTRSALAQRAAEAGRPEVAALFKAERNPSAVKDSPAWAAAR
                     EMFYATTNRVTGRFDAGLNLANHLDFEYLPGRKYDTFYDPEGLLSTLPAVATCLLGVF
                     AGLLLRMPTLAGRQKVAWLIAFGLAAATLGWLWDAQFPVIKKIWTSSYVLVAGGYSAI
                     LLGLFYLVVDLWQARAWCQPFVWMGMNSITIYIAANILGGFRRLAPRLAGGDVKAFFD
                     ERVAQGCGELVISLVGLLLAFWLVRFLYQRKVFLRL"
BASE COUNT         2639 a         5485 c         5929 g         2883 t
ORIGIN      
        1 tgttcttgcc atgcctctca atacaatgca actactaaca ggaaatggta taaatcacgc
       61 cgccgggtga cgcggcttga tttacgaggc cggaccgcaa gcccaggcct tttgggtgcc
      121 gacgtggggt ttgcgggcgg cggagggagc gatgcgggcg agggattggc aggttgctgg
      181 tttggcgcag ggggtgaggg ctttccgcgg gtgggcaggc ccggcaactc tgttctggcg
      241 caccgccgct tcgagggtta gataggcttc gaatacaccc gctgaaaccc tttccgtcct
      301 ccggcgcgat acgggtccac gatggtgatg ccgcgcctgc cgaagtgggc ggtgacgggc
      361 gcgcctttgc cggtgcgcag gtatttgaag ttcaggttgc ggacgaggcg ggcggcgtgg
      421 acgatggcca cagtgatgcg cgcttcctcc gcggtcggat acaggctggc cacgctggcg
      481 cggcgggcgc caaagtattt cacgcggcag acagccgcca gcgccgccgt gaggctgtca
      541 atgccgtagc cgacgtaggc ggtggaggcg tccggccgct tcacgttgcg cgtgaagtgg
      601 gtattggcgg tgcggctgcc gccgccggcg ctccaccagc gcaagccgcg gtattgctgg
      661 tcgctctcga ccttgccgtc cgtgccgacg atttcgtggc cctggttgac cggcccctcg
      721 aagccgggcg gggtgatcca gttattgtgg aaggtgacgc tcaggccgtt ggcgaagtca
      781 acccgcacct gcaccgcgtc gtaggcattg atgccgtcgc gcaccagccg ccgtttctgg
      841 ccgacggccg tgagcgagac cggcttgctc cggtagtagt ggtaaattaa atccacccag
      901 tgggagccga cgtagctgaa cgggtcgctt tgctcgaccc atttgaacgc gcgggtcgcg
      961 acccgcagcg gctcctccag gtaggccgag ccatagagcg gcgcgccgag gcgttgctgg
     1021 atttcctcgc gaatgcgcgc gtggtcgggg tcgtagcgtt tgtgcatgtc caccgcgacg
     1081 atgcggtttt tccgccgggc cagcgcgatg atttgatccg cctcggcgat gtccaggcac
     1141 atgggttttt cggtgatgac atggaccccg gcttgcaggg cggcgaggat gacctccgcg
     1201 tgcagcgggt cgggtgtggc cacggccatg acatccaggc ggggaaaggc gcgcaggatc
     1261 tcgcgccacg gcttggggcc ggcgaaggcc cggggcgcgt ggcccgtcca gcgctggaag
     1321 tttcgccggg cgcgccgggc ggatgtttcg gtgcgcgccg ccaccgcggc cagctcgaac
     1381 cggatcgggg ccaggtcccg cgcccagccg tccagtccca cccgcccgag ttgcgggctg
     1441 agcccggcgc gctgcaaatc cgcgtaggcc cgcgcatgca cgtcgccgcc aaacattccg
     1501 gttccaacca gcgcgatctt gattgagttg tccatagcta aagcggcccg ggcgttcggc
     1561 ccggccgggg ttgctgccgg ctgagagtgc cgcgttggcc cggcgggggc aaaccatttt
     1621 ggcgccggct tcggcgggga aacccaatcc cgccgggggc ggggggccgg cccggcccat
     1681 cgccggcggg cggtttcgcg cgtccggttt cgccgcgttt ctccgcgcgg cagcccgcgc
     1741 cgggggttgg ccggggattg ccattgtcag cggggcaggg ggccgataga gtaaagacgc
     1801 gcgatgattt cggcccaaca acaggcgacg cttagcgggg cgaactgcga aattgccttt
     1861 gataatctga cgcgccagct ttacgcgacc gacgcctcga tctaccaggt cgttccggcg
     1921 gcggtagcct ttcccgccaa tatccggcag gtcgttttgt tgttggatgc ggcggtgatg
     1981 gcgggggtga ccgtgacggc gcggggcgcc ggcaccgggc tggcgggggg cgctttgggg
     2041 gacggcctgg tgcttgattt ctcccgctat aaccgccaga tcaccgacct ggatttggag
     2101 cgccggacgg tgcgggtggg gccgggggtg gtgctggatc agctcaataa cttcctgcgg
     2161 ccgcacaaat actggtttgg gccggatgtg gcgaccagct cgcgcgccac gctgggcggc
     2221 atgatcgcca acaactccag cggcgcgcac gcgccgatct atggcaccac cgccgaccat
     2281 gtccaggagc tggaggtggt gctggcggat ggccgggtga cgctggtggg agccaaacac
     2341 gacacgctgc gcaagcagcg cgagcgggtg agcgacctgc tttattttca cggcctgagc
     2401 attgcggaac agcggccgcc gcagttgacc aaacgctggc cgggctatgc gattgagcgt
     2461 tgcgcgccgg agccgaacaa cctcaacccc ctgctctgcg gcagcgaggg aacgctggcc
     2521 atcatcgtcg ccgccgagct gaacatcgtt cccctgccgc aacaaaaagg cctggggctg
     2581 atcttttttg cctcggtgac ggaggcgatg caggcgacgg tggaactgct ggatctccag
     2641 cccgccgcca ttgagcatct tgaccgggtg ctcctggatc agacgaaggg ccagccccgc
     2701 ttccaggccg cgcgcgacct gctcgagctg gatgccaagc cctgccaatc cattttggcg
     2761 gtggagttct ttgatgacgt ggaggagcgt ttggcggcgc tggagcggcg gcggctgggg
     2821 ttgcgccaga cgctgctcaa gaccgaggcg cacatgaagc agatctgggc gctgcgccag
     2881 gcggggctct cgctgctcac gggccgcaaa ggcgaggcca agcccgtcac cggcatcgag
     2941 gatgccgcgg tgcggccgcg ggatttgccc gcctacgtca gtggtttgca gtcgctcctg
     3001 gcgccgctgg ggctccaggc ctcgttctat ggccacgccg ccgcggggct gctgcatgtg
     3061 cggcccatcc tcaacctggg ttcggcggcg gacgcgaaga agtttcgcca actggcggac
     3121 gaggtctccg ccctggtcaa acggttcaaa ggctcgctgg cggccgagca tggcgtgggc
     3181 atggcgcggg cggagtatat gccgcagcaa ctgggcgatg ggttgctgag cgtcatgcgc
     3241 gagatcaagt ccgcctttga cccgaacaac ctgcttaacc cgggcaaaat tatcccggat
     3301 gggcggttta aggtggatgc cagcttgcgc cgggggccgg gctatgagct gaagctcccc
     3361 ttcacgccgc tgctggcgtt tgccgccaaa gacgggtcgt tcacgcgcaa cctggagcaa
     3421 tgcaacggct gcggcggctg ccggaaggag acgcccacga tgtgcccgac gttcctcgtc
     3481 acgggcgagg agattatgag cacgcggggg cgggccaatg ccatccgcgc ggcgctggag
     3541 cggcggggcg gggcgggggg cgatgtgctg cgcggcgcgg agctggaagc ggcgctcagc
     3601 aactgtctgt cctgccgggc ttgcaccctc gaatgcccct cgaatgtgaa tatgtcgctg
     3661 cttaaggccg agctgctgca cgcccggatt cagcgcgacg gcctgtcctg gcgcgagcgg
     3721 ctgatcagca aggtggattt gctcgggcgg ctgggctgcg cctggccgcg gctggccaac
     3781 gcggcgctgg cttcgaactt cgtgcgccgt ccgctgaacc ggatgctggg cctggcctgg
     3841 cagcggccct tgccgcgcta cgcccggctg cgttttgacc ggtggtttgc gcggcgcggc
     3901 gcctcgcccc aggccccgcg cgggcgggtc gtgctgtggg atgacacgtt tgtgcgctac
     3961 catgagccgc acatcggcag ggcggcggtc aaggtgctgg aggcggccgg gttcgaggtg
     4021 acgctgccgg cggggcgaaa gtgttgcggc cggccggcct tcagccaggg caatttggcg
     4081 gaggcgcagc gcctgggacg ccataacctg gccttgctca gccgggatgg ggatggcgcg
     4141 cccatcattt ttctggagcc gtcgtgctat accatgtttg ccgaagacta ccgcgaactg
     4201 aagctggacg gggccgagag cgtggcgcgg cgttgttttc ttttcgagcc gttcatcgag
     4261 ggactgctca gccacgagcc ggacgccttg cggtttaaga cccgggcggg ccacgtggcc
     4321 attcacgccc attgccatgc caaggcgctg ctggaccccg cctgcctgca gcggctggcg
     4381 gagcggctgc ccgagcgcaa cgtgaccctg ctggagaccg gctgctgcgg catggccggc
     4441 gcgtttggga tgatggcgtc caaatatgag ctgtcgctca aggtcgccga gccgctgatg
     4501 cagaaggtgc ggagcctgcc gttcgggacg ctgatcgtcg cctccggcac cagttgccgg
     4561 cagcagattg accacctggc gccggtccgc gcccgccaca tggccgaggt gctggcggat
     4621 gcgctggcgt gatcgccggc tccgcgcgcg ggcggttggc ggcacggagt tccaacgggc
     4681 tgggttgccc gcccgggaga cgccgtctcc cggagggaaa ccagggttgc gcggcgcggg
     4741 aatgtttgtg aaactgtcac gcatgaaacc aggccaggct gtccggagac tgctgcccgt
     4801 gtgggtggcg atgcttgggt tgctcggcgc gccggaggcg ggcgtcggcg ctggcgccac
     4861 ggcgggctgg gcagaagttg agattacgcc gcccctgggc atcgcgctcg gcggccgggc
     4921 cgggccggtc acgcccgccg acaaggtgct ggatcccttg ttcgcccagg tgctgtgcct
     4981 gcgggatggc ctggggaagg ggttggttct cgtctcactc gatctgatcg gcctgccgca
     5041 tgacttgagc gaccgcctgc gcgcacagct tgtgcaggag ctgggagtgg gatggaacct
     5101 ggtggtgctg aatgcctccc acacgcatag cgggccgtat atgctgcgca gcctggtggc
     5161 gggacttggc ccgccgccgc cggtcgaggc ggaatacttc gacacgctgg aagcgaaggt
     5221 gatcggcgcc gcgcgggcgg ccggccgcgc cctgcggccc gtgcaagtgg aggtgtttca
     5281 cggccgcacg aagctgggca tcaaccgccg cggcataaat aaggaagggc gctatgcggg
     5341 gatggttccc aatgaaaacg ggccgcgcga tgaccgggtc tgggcgatga aattaacgcc
     5401 cacgcagggc ggggccccgg cgctggtgtt ttcctgcgcc tgccatgcgg tgattgtgta
     5461 cgggttcgat tatgccgccc tctcggccga ctttcccggg gcaacccgca atcttctgcg
     5521 cgccgcgctc gatcccaaag cgcacctcca gtttgtgcag ggctttgccg gggatgtccg
     5581 gccgcgcgtg ctggcggacc tcgagcgggg ccgtttccgc aaagccatcc ccgccgacct
     5641 ggagcaggcc gcgcgcgaat tgtccggcgc cgtgctgtcc gcgttgaaga ccccgggaga
     5701 aaagctgtcg ctggacctcg cgggcgccag cgaccggcct tacctgccgc ggggcgcgcc
     5761 gccgccgcgc gaacgctatg agaccatgcg cgccacggcg ctggcgcaga ccaacgccca
     5821 ccggctcgcg ctggcggagt actggctccc gcgctacgat gcgggcgaag gcttcgcccg
     5881 cggcaatgcc tggccgctgg gcctgattcg attggcggag aaccagtgga ttgtgcattg
     5941 cgccggcgaa ccgtgcagcg cgtggcgggg cctgatggcg caatggctgg cgccgttgaa
     6001 cctcgtggcc tggggataca gccaggaagc gcagtcgtat ttgccgaccg aatccatgct
     6061 gcccgaggga ggctacgaag tggtggattg caacctgggg cgaaaggccg gttcggcgcc
     6121 tttggcgccg ggcatcgagg cggcggtgcg ccaaagcctc ttgcggcaac tggcgttcat
     6181 tcgcgccggc gcgcggtgaa gcaatgccgg aaaggggctg gccgccgggc gtcccgccgg
     6241 ggcggagccg gctgccgggc gcggggcatg cgggaacggg caaactttcg cctttgaact
     6301 ttgccggcgc ttgcctataa ccacggcgtg tccgggcagt ttcggcaacc catagacgcc
     6361 agcacgcgct tttgcgccgt gtatggctat cccgtcaggc actccgcttc gccggcgatg
     6421 cagaatgccg gcctggccgc gctcgggctt aactggcgct acctcgcctg cgaagtccgc
     6481 ccggaagacc tccgcgcggc catccagggc gcgcaggcga tgggcttcct cgggctcaac
     6541 ctgaccgtcc cgcataagga gatggcgcgg gacctggtgg atgcgctgga cgcctcggcc
     6601 cggacttggg gcgcggtcaa caccatccgc ttcgagggcc gcgacgagca cggcgcgtgg
     6661 cgggctttgc gcgaattttc cgcccctccc cggcaaaccc gatcgcaggg ctttaatacc
     6721 gacgcggacg ccatcgtccg ctcgctgcgc gaagatttgg ggctggaact gcgcggggcg
     6781 aaggtcctgt tgctgggcgc gggcggggcc gggcggacgg cggcgttgaa gctcgccgcg
     6841 gaaggcgtcg cggagctgtg cctggtcaat cgcacccgcg gcaaggccga agcggtcgcc
     6901 acagaaatcc gggagcatta cccggaggtg cggctggccc tgggctatcc cgcgggcccg
     6961 gtggaattgg tgctgaatgc gacttcgctg ggcctgaagg cgggcgatgc gctgccgctg
     7021 gacgccggcc agttcccgtt ccggcgggcg ggggccgcgt atgacatgat ttaccggccc
     7081 gcggaaacgg cgtttctcca ggcggcccag gcggccggct gccgcgtcgc caacggcctc
     7141 ggaatgttgc tctaccaggg cgccgccgcg ctcgagttgt ggacggggca gccggcgccc
     7201 ctcgggaaca tgcgccaggc gctggaaagg aacatctacg gctgacttat cgctcatgct
     7261 gactcccaac ttcgacccca acctctgggc ggccgttccc tttcatttct ggtcggtggc
     7321 aagcgtggtg ttaggctcca tggtcggcag ctttcttaat gtctgcattc accggctgcc
     7381 gctggagcag agcgtcattt cccccccctc ccattgcccg cattgcaact attccatccc
     7441 ctggtatctc aacatacccc tgttcacctg gctgtatttg cgcggcaaat gccggaactg
     7501 cggcgcgccc atttccatcc gttatttcct ggtcgaactg ctgacggcgg tgacgtttct
     7561 gtgctgctgg ctgacctttg ggccctgttc ggcggcgctc gccctggtct atgccctctt
     7621 cctgtccggc ttgattgcgg ccaccttcat tgacttcgag cacttcatca tcccggatga
     7681 aatcaccatc ggcggcatgg tggccgggtt cgcgtgctct tttttctggc cggcgctcca
     7741 gggacaggcg tccccggcgg gcggcctgag gcaaagcctc ctgggcatgg gggccggcgc
     7801 gggaatcatt tacttgattc tgcgcggcgg caagctgctg ttcggccggc aacgcgtcac
     7861 gctgcccgct catacccggg tggtcttcgg cgagagcggc gttcacctgc cggaccggga
     7921 aatcccttat gacgagctct tttatcgccg ttcggacgtg attaccttgt acgcccgcac
     7981 gctggaactg gcggatcgcg gctacaagga tgtccgggtg cggctggcgc ccgaccggtt
     8041 gcagatcggg gaggaggaat tgaatccgga ggaggtgccg cacctggagg ccgtctgcac
     8101 cgagatcgtt ttcccccgtg aagccatggg cctgggggac gtgaagttca tggcggccat
     8161 cggcgccttt ctggggtggc cggcggtggt cttttccctg atggtcagtt ccatgatcgg
     8221 ggccgtgatc ggcgtttccc tgattctgct gcgcaagcgc gaatggtctt cgcacctgcc
     8281 gtacgggcct tacatcgcgc tggccgccac cgtctggatt ttcgggggca aacgcctggt
     8341 ccggtggtgg ctcgggctgg aatagcgcgg gtcccgccgt ccgggaagcc cgcgggggcg
     8401 ggcgggcggg gcggcgtcag gcggcggcca gcgcccccgt ttccacccag gctttccggc
     8461 cgccgacaat cgtggcgacc gccttgccta gcagcggcca gccgtaaaag gggctgttcc
     8521 gggctttgct ggcggtagca gccggctcga atacccacgc gcgatccgga tcaaacaccg
     8581 tcacgtccgc caccgcgccg acgctcagcg ttccgtgcgg caggcgcagc cgccgggccg
     8641 gcgctaaagt gaacttggcg atgacctccg ccagccccag ccgcccgctg tggtaaagct
     8701 gcatcagcgc cagcgccagc gcggtttcca ggcccgtaat cccgaacggc gcgtaatcga
     8761 actccacctc cttctcgtaa tcacagtgcg gcgcgtggtc gctgcccagg atttccagcg
     8821 tcccatccgc caggccttcg aggatggcct cgcggtcgcg ggccgtaccc aggggggggt
     8881 tgactttgaa gttcgtgtca tacgccggcc aggccggccg gggccgggag ccggagccaa
     8941 ggccgaaaac gcccggccca tccgtttcca ggaacttctg gctgccggcg atcgccgcat
     9001 ccgtcagggt gaagtgatgc gggcaggctt cgccggaaat gggcaccccg cgccgcttcg
     9061 cctcccgcag caggcgcacg ctcccggcgg cgctgatatg ctggcaatgc acctgcgcgc
     9121 ccgtcatttc ggccagcagg atgttccgcg cgacgatcgt ttcttcgccc gccgccggcc
     9181 agccgcgcag ccccagtgcc gtgctccaat agccctcgcg catgacccct tcggtcacca
     9241 gcgaataatc ctggcaatga tccagaaccg gcagctcgaa catcttcgca tactccatcg
     9301 cccgccgcat cagctccgtg ttttgcacgc aatgcccatc gtccgtaatg gcggccaccc
     9361 cagccttttt cagcgagcca atcggcgcca gttcctcccc cgtcagcccc ttggtgatcg
     9421 cccccgcgac atagacattc acgactccct cccgggcggc gcgctcgcgg ataagcgcga
     9481 ccgtcccggc attgtccgtc gccggggaag tgttgggcat gcagagaatc gaggtgaacc
     9541 ccccgcgggc cgccgccgcc gtgccgctgg caatggtttc cttcgcggtc tggcccggct
     9601 cgcgcaagtg cacatgcata tctatcaggc ccgggcaaac cacccgcccg cccacgtcca
     9661 gccgttccac accggcggga gcctgccccg ccacagccgg ccccacggcg gcaatcttcc
     9721 cgtcgcaaat cagcacctcg gcgcgcgcgt cgaaatcatt ggcggggtca atcacccgtc
     9781 cgccggtcag cagcagcgaa ttcatgcagc gcagcttaaa ggccggccgg agaagttcaa
     9841 gctccgtccg gggtcaatct ccggcattcc ggcgcccggg taaaatccgg ccagctcggg
     9901 ggcggccttt attcccgcgc ggccgcggcc cgcggcgctt cccggcgggc gggcgttggg
     9961 ggggaaaccg cttttagcag aaacaggcct tccgtatcca ccagcgagac caattccagc
    10021 cccgcccgcc ccagcagtgc gggccaggca gccgcggctg gcaggaggtc gtggcagacg
    10081 ggcgccgcaa gctgggaatg gaaatgatta agctccgcgc tgccggccag gtgcagaata
    10141 atcaactgcc cccccggccg gagcaggcgg cgcaggttgc ggagggcgcg cggttgatcc
    10201 cggaaatgtg gaaaggcgtg gaagcacagc gccaccgcga attgttcggc ggccgcaatc
    10261 tcgccgcaaa catccagcca gcgaaattcg gcggcgaggc ggcgcacggc ggcctgggcc
    10321 agcatggccg gggaaaaatc caccgccacc acccgccccg ggcgcactgt ctccaccagc
    10381 cagccggtaa tccgtcccgt gccgcacccg acttccagca cgtcctggcc gggaaccagc
    10441 ccaatccgcc cgcgcagttc cgccagccgg gccagcgtgc gcctgacgtc gtcggcgtcg
    10501 tcgtcccagg tcggggcgcg ctggtcgaaa aaggccacgc gtgggtcggg cggcggcggg
    10561 gccggggcgc gccggtgatt tcgggcttgt gcccgcaggc cgcaatcagt aacactcatt
    10621 ctcatgatcg taatactgcg ccccggcgcc cgtcaagcct caccgcgccc gccccgggca
    10681 atccgggggc gcgggcggcg gcgcgagagc gggcggcgcc ccgctgggga gcggcgaaag
    10741 ttctcaatcc tccccgcgcc gttcatgcca aagctgacgg tcttgctgct ggcggggctc
    10801 ctgggggcgg cgggcggctg ccaccgcgcc gctcccgcgg ggcgggggcc ggtgattgcc
    10861 tccgcgactt cctacctgga aagcgccgcc cgggacgtgc tgggcgacga ctttgacgtg
    10921 ctccggctgg cggagccggg cacctgcccc gggcatttcg acattcgccc cagccaggcc
    10981 gcggccctgc gccgctgccg cgcgctgctt cggtttgatt tccagtcctc gctcgaccgg
    11041 ctgctggaac ccggcggcac gaaccgtcca ttggtggtcg ccgtcgcggt tcgcggcggc
    11101 ctggttcagc cggccagcta tctttccgcg tgccgccagg tggcggaggc cttcgtggcg
    11161 gcaaatcaac tgccgcgcca gcgcgccgac ctccagttgc agcggattgc cgcgcggttg
    11221 gaagcgctgg cgcaagaggc caccaaccgc gtggcccagg ccggcctgcg cggcgcgccc
    11281 gtcatcgcca gcgttcacca gcgggacttt tgcgaatggc tcggcttgcg ggtcgtggcc
    11341 gcgtttcgcg ccgccgacac ggccagcgtg gcggaaatag acgaggccat ccgcgccggc
    11401 aaacttgccg aggcccggct ggtcatcgcc aaccagcccg aaggccggcg cacggcggac
    11461 gccctggcgg agcggctcaa ggcgcgcgtt gccgtcttgg gcaattttcc cgcgggggaa
    11521 cacgggcggg cctccttcga caatttgctc accggcaatg tgaacgcgct cgtgcgggtt
    11581 tcccggccat gagcccgccc gccgtcgccc tggaaaagct ggtggtgcgc gccgggcgcc
    11641 ggcggctgct ggatgtggcg gaattgcgcg tgccggcggg agaaatcgtg gccgtgatcg
    11701 ggcggaacgg cgcgggcaaa accacgctgc tgcgggcgtg cctggggctg gtgaaacccg
    11761 cgcagggcgc cgtgcgcgtg ctgggccagg aggtttgggg gcgcgacgcc ggcgcacggg
    11821 cgcaactgcg ccgccgggtc ggctatgtgc cccaactgct gcccgcccgc agcgaaatgc
    11881 cgctgacggt gcgggaggtg gtggcgattg gccgcaccgg gcgggcgggg ttgtttcatc
    11941 cgctgcgccc cggggattgg cagttggtgg acgaatggat agagcggctg gggctggcgg
    12001 cgctggcgcg gcgcgcgttc agcgaaatct ccggcggcga gcagcgcaaa accatcatcg
    12061 cccgcgcgat ggcgcagcaa ccggagctgc tgctcctgga cgagcccacc gccaatctcg
    12121 acctgggctg gcgcgagcgc atggtcgcgg ccattcagca actgcacgcg gagctggcca
    12181 tcagcatcct gctcgtctgc cacgaattgg aggtcctgcc gcctgcgtgc cggcgggtgg
    12241 tgctgctgga cggcgggcgc gtgtgggcgt ccggcccgcc ggaaacgatt ttcacggacg
    12301 aacgcatcag cgcgctttac ggcgccgggt tgcgggtggc gcatgaaggc gggcggcatg
    12361 ccgtcctgcc ggggagcggt gcgcatgttt gacttcgtct tctggctgcc cgtgatggcg
    12421 ggaggggcgc tggcgggcgc ctccagcggc ttgctcggca ccttcatcgt cgggatgcgg
    12481 attccgttcc tcggggtatg cgtggcgcac gccgcgctgg cgggggcggt gtttggcagc
    12541 ctgggcggcc tggcggggca agccttgctg ctgcccgcgc tgggcgcttc ggttctgacc
    12601 gcgctggcgc tggggctgct cgatccgcag cgggtgcggc tggacgacaa tgcggtcctg
    12661 agtttcctct tttccgccac catggggctg gcctttctgg gcattgggct tcatgggata
    12721 ctcgggcgca gcgacaacga cgtgcgcagc atgctgtggg ggagcctcaa cttctgccgg
    12781 tggcgcgatg tcgggctcat gctggccagt tccggcgccg tgctggccta catcgtggtt
    12841 ttcttcaagg aactgcgcgc tattatgttc tcgcgcgccg acgcggaagc cgccggcatc
    12901 cgggtcaagg tggtctggtc gggtttcctc gtcctggtgg cggccgtgct gaccgtcaat
    12961 ttccagaccg tggggggcct gatgatctac agcttgatca gcaacccggc ggcggcggcg
    13021 tttcaactcg tgaaaagctg ccgtcgaacc ctggccgttt ccgccgtgct gggcgcggcc
    13081 agcggcctgg gcggtttcct gatttccgcc gccaccgacc tgcccagcgg ggcggtgatc
    13141 gtgctggtgt cttccgtgct gctcgccctg gcggccatcg tccggcgctg ccgctgaggc
    13201 agggccaaca aaaagggccg ggtcactgga cccggcccgt tgaatgagga ttggctatgt
    13261 cagccgcgtc ttacttgctc tcgatgcggt agaagccagg ccccggaacc gcgaaggtgc
    13321 ccggcgtggc gttgttggtc gccaccggga cccaggtgtt catcggggcg ttcacagccg
    13381 ccgccttgag cagaatgaac tgcgtcccgg cgcccgtgct gtaggcaatc gtcgtggccg
    13441 tgatgctgtc tatcttcacc ggctcaacgg ctggcgcgct gaccgtgagc acgcccggcg
    13501 agctctcgat ggaaccgcaa gcggcgttgc tcaccacgac cgtgtagttc ccggcatcgc
    13561 cggcactcac cgagccgatg gtgtagctgc tgctgtcagt gccgacgttg ctgccattgt
    13621 gcttccactg gtaggagaac ggcccgacgc cagtggcgac aacgctaaac gtgacgctct
    13681 ggcccggcgc cttggtctgg ctggcgacag ggccgacgcc cagcttggcc gtggtctcgc
    13741 ccaccggcag gaccagcaaa cgcagggtca ccggcttgct cttgtcgagc tgcgggtagg
    13801 tcgcgccgtt ggagcggttg gccaggacgc gcacccagcg cgtttgccct tcattcttga
    13861 attgccaccg gatgcggcag gagttcgtgc cttcataggc attcgcctgg ctaatcacgg
    13921 aagaagtagt tccgccgccc aggacgtgga tggcggggtc aggaacgcca gtggcattcg
    13981 gcgccgcgaa catcacgctg tcaccggcct cccagccctc gaagtcctca atgaccacgt
    14041 cgccattcat gatcttgtcc acatagatgt cgtaggggcc cgtatcggga tggtcgggat
    14101 caatcgcgaa ggagagggca tcgaaggtgc agaacggatc gctgtcggaa tacgcagtcc
    14161 ccgccatcca gttgagcatc ggatcagtgg cgaaatcaaa cgtcaccgtt tgccagcagg
    14221 agccgggcac cagcgcaacg ccgcccacgg gcgcagtagc ggagccgccc gccagcccgg
    14281 tggcgttaac gtggtacagt gtcgtggccg cacccgaggt atccgcgccc gcggggccgg
    14341 tcagcgccga ttgcgcgccg caggacagca cgaccttgag cgccgggttg gcgccactgc
    14401 ccaccagcgg gccgtcagcc ggcagcgggg aaacgcagta cccctccggg gccgcgcttg
    14461 gcttcttctg cgtcgctttg atgatattgc ccttgaccag gccggtgttc accgggacgc
    14521 tgagcgcgcc ggccgcaaag cccgcggcga ggttcgtctg gccgatttca gcatcgttcg
    14581 caaaaatcgt gacattcgtc gcgcccgcag tgacccccga gaccgtgaca aacatttggc
    14641 cggccgcgag cggtccattg acggacaggt cgccgacgac gccggcgcac gcatcaataa
    14701 gggtgaactt aaaggcgtcc acgtagaggc gcttttcgtt ggagcccggc ccggtaacgc
    14761 cgcccacctg cttaaagatg atctccggat ccgtaatgcc cggggcattg gtgatatagc
    14821 ccatcaattt ccacgtgttc ccgccgtttg cctggttaaa gacggcggaa ttggtgcatc
    14881 ccgcggacgt ttgaccatag aagttgtctg cgcagtaggc tgtgaccaca acgtccggcg
    14941 aggtcgtcag cgtggtatgg gcaacttcga tgctataaac gccgccatca acccccagcg
    15001 gcgggacgat gagccagttg ggattcaagt cctggccgaa gtaaaagacg catctcgtac
    15061 gggaggggac gcccggcgga ttagcaatcg cagttgaccc gtatggcgaa taagaaggcc
    15121 ctaaacccaa ggggtcattg aaaaaggttg tgctattaag agtccccccg agataggtgg
    15181 tgaggtaaac tacatcagcc cgcagcggtt ggaccgccag tgagccgatc gcgaggagag
    15241 ccagagttgc ccggcgcaac ccgacccaac aagaggattt tgttctatac ataacaggag
    15301 ttggtttact gtttcgttat tgtttctttc gttttcagag tccacagact catgccatta
    15361 cctgcaatat gaagaataag accgaaaagg tcaacccttt tccgccaaaa aatgcggatt
    15421 tatttgcaag ccgccccacc cgcccgggaa ggccgggcgg gaggttcgaa aaggcggaag
    15481 agccgcgcgg gggcggaggc ggagcggtca gaggcgcagg aagaccttgc gctggtagag
    15541 gaagcgaacc agccagaacg ccagcagcag gcccacaagg gaaatgacca gttcgccgca
    15601 gccctgggcg acccgttcgt cgaagaacgc cttgacgtcg ccgcccgcca gccggggggc
    15661 caggcggcgg aaaccgccga ggatattggc ggcgatgtaa atcgtgatcg agttcatccc
    15721 catccagaca aagggctggc accaggcgcg cgcctgccag aggtccacga cgaggtagaa
    15781 aagccccagc aggatggcgc tgtagccgcc ggcgaccagc acgtaggacg aggtccagat
    15841 tttcttgatg accgggaact gggcatccca cagccagccc agcgtggcgg cggccaggcc
    15901 aaaggcaatc agccacgcca ctttctggcg gcccgcgagg gtgggcatcc ggagcagcaa
    15961 gccggcaaac acccccagca ggcaggtcgc cacagcgggg agggtgctca acagcccttc
    16021 cgggtcgtag aaggtgtcgt acttgcgccc cggcaagtat tcgaaatcca ggtggttggc
    16081 caggttcagc ccggcgtcaa acctgccggt gacccggttg gtcgtcgcgt agaacatctc
    16141 ccgcgccgcc gcccacgccg ggctgtcctt gaccgccgag gggttgcgct ccgctttgaa
    16201 cagcgcggca acctcggggc ggcccgcctc cgcggcgcgc tgggcgaggg cgctccgggt
    16261 caacgggagg tcgcgaaccg gcacgaacgt catcagcgcc cagtagcccg ccagcaggcc
    16321 cacgcaaatc gccgccagcg cccgcggccg gaaaaagcaa aacagcgcgc cggcgaagaa
    16381 atacgccagc gcaatccggt tgagcacgcc catcagccgg aggtcgggcc agggattcgt
    16441 caggccgccc gagtagaaga tgccgatgac aaacagcagg aggctgcgcc gcacgatccg
    16501 ctggattgcc cgggcccgcc cctccagggc aatagtccgg gagagggaga acaccagcga
    16561 gaccccgacg atgaagatga acagcgggaa gatcaggtca tagaaacgga agcccgccca
    16621 ttcggcgtgt tcgagctggc gggccaggaa ccgggtgggc ggcgtcggcg acatgcggtt
    16681 cagcgcatag accagggaat cggcgccaat gatccagaac atatcgaagc cccgcagcgc
    16741 atccacggac atgagccgct ggctgggagg agtcttttcg gtcagggttg ccatacgcga
    16801 ctgagttttg gacatattta gcccagacat ttcacacctg caagactgga gcgcgggcat
    16861 cctgcccgct tacagcctcc caacgggcta agcgggccag aggcccgcgc tccggcctgc
    16921 gggtatttcg tgcggg
//
LOCUS       DAOPQG010000080        16643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4138_length_16643_cov_15.372257, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000080 DAOPQG010000000
VERSION     DAOPQG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 16643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16643
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4138_length_16643_cov_15.372257"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKI07_09750"
     CDS             complement(<1..451)
                     /locus_tag="PKI07_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOC51374.1"
                     /translation="MSESDWFQGEYNFLGLPAELTQRQRARAWVLPVPYDATTSYAPG
                     TRRGPAAILAASREVEQYDREFDCEPAVAFGVHTLPPLRAELQSPEAMIERVAGAVTG
                     LLRGKPAPELLVVLGGEHSISAGVARGLAQARGARDLVAVQVDAHADL"
     gene            complement(634..2022)
                     /locus_tag="PKI07_09755"
     CDS             complement(634..2022)
                     /locus_tag="PKI07_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOC51375.1"
                     /translation="MAKQTQSQERARAAAGGRRGRGMLLLSAVVAALGSLLFGFDTAV
                     ISGTTEALRQVYALNDNLLGFTVSSALIGTMIGALLAGRPADWWGRRPVLALLAVFFA
                     LSAVGCALAWNWHALLFFRWLGGVAVGGASVVCPMYITEIAPARLRGRLVAVSQLNIV
                     VGILAAYFSNYLVARGLGAEHPQAWRWMFGVMTAPAAAFLVTALLIPESPRWLVKRGR
                     RAQAEAVLARFGHEHPAGEAREIADSLKAEIAGARPRLFQRQYLKPLLLACMIGAFNQ
                     LDGINAVIYYTADIFRMAGADQTSALSQSVIIGFTNLAMTILAMALIDRIGRKALLLI
                     GSVTFVLSHALAAWVFATHAQGWIVIVAMMGVVGSHAYSQGAVVWVIINELLPNAVRA
                     SGSAVVCFLIWVLCAGVSWSFPVVAARSGAYAFGFFALMMVLQFILVARFLPETKGVS
                     LEELQQRLGAAD"
     gene            2210..4969
                     /gene="acnA"
                     /locus_tag="PKI07_09760"
     CDS             2210..4969
                     /gene="acnA"
                     /locus_tag="PKI07_09760"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOC51376.1"
                     /translation="MSHTHNRFGALQTFDPGNGRPGALYALPELERAGVGPVSRLPVS
                     LRLVLESVLRNCDGRRVTETNVRALANWEPHAPRREEIPFVVARIVLQDFTGVPLLVD
                     LAAMRSALARLGRNPGLVEPLVPVDLVVDHSVQVDYHSAPDALERNMALEFQRNRERY
                     QFLKWGMQAFKKFKVVPPGIGIVHQVNLEYLAQGVASAPLPGGAPAPALFYPDTLVGT
                     DSHTTMINGLGIVGWGVGGIEAEAGMLGQPVYLLTPDVVGVHLFGELREGVTATDLAL
                     TVTQRLRQARVVGKFVEFFGPGAAALPVVDRATVANMAPEYGATMGFFPIDAECVHYL
                     RATGRGEEHCRAYENYYRAQGLFGMPQKGQIAYTQELELDLATVTPSVAGPKRPQDRI
                     ELPNLKREFEAALARPAAENGFGRAAAELAQSHPLPHGGGALRHGSVMIASITSCTNT
                     SNPSVMLAAGLLAQKAVARGLRVPPWVKASLAPGSRVVSDYLRRTGLQQPLDQLGFSL
                     VGYGCATCIGNSGPLPAGAEDLISRHHLVVAAVLSGNRNFEARVHSSLKANFLMSPPL
                     VVALALAGRVNIDLSTEPLGRDKDGREVYLKDIWPARDEVREAMRAALKPEVFRQLYR
                     DLAAQVPQWSQIPARGGEVYQWDPDSRYIQEPPFFANFSLQPGATSDLRGARALAIFG
                     DSVTTDHISPAGSIKPASPAGKWLLERNVTVADFNSYGSRRGNDRVMTRGTFANVRIR
                     NLMVPGVEGGVTRCFARSDNPGAAGEPISIYEAAMAYQREGTPLLVLAGQEYGTGSSR
                     DWAAKGPALLGVKAVIAQSFERIHRSNLVGMGILPLQFKDGTSAQTLNLDGTETFDIS
                     GLAHGLKPQQELTVSVRRANGREEAVPVICRIDTPIELEYYQHGGILPYILRQLAGAS
                     GPAPA"
     gene            5948..6850
                     /locus_tag="PKI07_09765"
     CDS             5948..6850
                     /locus_tag="PKI07_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HOC51377.1"
                     /translation="MTTSKPGETVEPGALGRVTFLDGKGDLPMLEISTRWSAAEIYLH
                     GAHVTHFQKKAEPPMLFLSQCSRFAEGQPIRGGIPVIFPWFGRREGLGQHGFARLKTW
                     ELKEVVPAPDGSVSVRLRLPDGPEAAGFPRFTAEYIVTVNQALTLQLVVANPARDAEF
                     TFENCLHSYFEVGDIATASVVGLKGLAYEDRVGACVAGRESADALRVAAEVDRIYQDS
                     TGPLEILDPSLGRKIRIEKQGSASTVVWNPWVAKSRQLADFGDEEYRRMICVESGNVG
                     PASIRLLPGAASTLTVKLSTEPLK"
     gene            6966..8015
                     /locus_tag="PKI07_09770"
     CDS             6966..8015
                     /locus_tag="PKI07_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOC51378.1"
                     /translation="MPLEYKDYYETLGVPRNASEADIRKAFRKLAREYHPDVAKNKKQ
                     AEEKFKTINEAYEVLGDPAKRKRYDELGANWREGANFRPPPGWQSFGRGRAAGGGPGG
                     EAHEFRFSGTGFSDFFEQLFGARAARGASGFGHGGGWGAEEMGAERGQDIEGDILVTL
                     EEAMRGAVRSVAVQRAVPCGQCGGLGHRQRRACAACGGTGQVVKTDTWQVKIPAGVTE
                     GQRLRLAGRGEAGVGGGGSGDLYLRVRLARHPDFRVDDHNLIYAAEVAPWEAVLGASI
                     TVPTLGGTVRIKVPPGTQNGQKLRVRGRGLPRRGGPSGDLIVEIRIEVPQQVSSAERK
                     LWEQLASESRFNPRQ"
     gene            complement(8138..8851)
                     /locus_tag="PKI07_09775"
     CDS             complement(8138..8851)
                     /locus_tag="PKI07_09775"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HOC51379.1"
                     /translation="MNFKTKCRPILLLAGAGLLLGANAAQAIVMSSLSQTVVTAFVDV
                     FPETTTDWSHTFCLPQFNPALGTLESVNILASQTMNMTGTIRNTSTRPQNFSLRAGSL
                     LTVDLPGGFGFLQPSPLATAEVYQLPSGGVADYGPVSSTDTVSYAYTRPQDLDWFIGT
                     GTFTLPGTTQTQEIILGGGGNIWARLNTVAGAAVEVQYTYRPSQSVPEATTFLDLSLL
                     LLLPLGAGIWRKLARPATA"
     gene            complement(8983..9057)
                     /locus_tag="PKI07_09780"
     tRNA            complement(8983..9057)
                     /locus_tag="PKI07_09780"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9022..9024),aa:Glu,seq:ctc)
     gene            9269..10411
                     /locus_tag="PKI07_09785"
     CDS             9269..10411
                     /locus_tag="PKI07_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51380.1"
                     /translation="MDSNRELASFAARLHREIGPGQDSPKQTRAAWPAFNPLALELFA
                     LQFAHNPLYRRFCEARGARPGTVADWTEIPAIPTAAFKAGPLSCLPAAARTAVFHSSG
                     TTGQTPSRHFHSADSLALYEASLRAWFDAHWPVAAGPWAWVILTPPPDQAPHSSLVHM
                     FETLRRARKPAAAAFVGRAAEDGAWVLDVDAAWPLLQSAAAGAQPVFLLGTAFSFVHL
                     LDDFAARGRGLTLPAGSCALETGGYKGRSRALPKAELHALMSRRLGLRPDRIICEYGM
                     SELSSQAYDGEVGPVAARALPGLASPGARRFRFPPWARVRIISPETGAAVEEGETGLI
                     CVFDLANVYSVLAVQTEDLGRRRGSGFELAGRAALAEPRGCSLMAV"
     gene            10421..11701
                     /locus_tag="PKI07_09790"
     CDS             10421..11701
                     /locus_tag="PKI07_09790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51381.1"
                     /translation="MNAREHAQHRARTLWLTGVLHAFTHLYQAVLLPLYLLIQKDFQL
                     ASVGRVTLLVTVMLAACFGPSYPAGILADRLNRKKLLAFGLALNGTAFAALGLAPSYA
                     WALVAVVVAGIGSSFYHPAATAMITRLFPGRTGKALGLVGIGSSVGFFMGPLYAGWRA
                     QMLEPVLGPAAWRRPVLELGLLGVITAGLFYWLADEEQPSAPAERKAVPSGRMFPTPA
                     LWFFFFAYCLAFSFRDFAGASMGSLSSLFLQKARGFTPQSTGVALSLLCIASVVSNPL
                     FGHLSDGGRRRWITLVLTIAAVLVAAFPHVPPAWTLPVLIVYGFFFMASYPMTEAALM
                     ESVPDAVRGRVFGVFVTAGGVIGNLSHWLVGAKIRHLGAAAQNVSAYFPIYGFVGVLI
                     LVALSGLLCLGPIRRREGPLPLATARAPSLPPQT"
     gene            11698..13074
                     /locus_tag="PKI07_09795"
     CDS             11698..13074
                     /locus_tag="PKI07_09795"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOC51382.1"
                     /translation="MNLPNYFLADLPPEAALTPALIDEACQTLRRNRECYLAHRSTQS
                     LVQVLCRVARDWLEPSDPFRQFALEQSPAATGFPRATLARGLDAFFKSLTPEHFHALL
                     EQDLGHVRRLDELTSAAPERAARRAALATAPELLAHITAGNLPSPALAAIVLGVLTRS
                     AQFVKCARGASLLPRLFAHSLYQAEPKLGACLELAEWAGGRQDLEAPLFAAADCITAT
                     GTDATLAAIRPRVPAAARFLAYGHRVSFAYVAAAGLAGRQARQIAAQAAADVAAWNQQ
                     GCLSPHVIYVELGGGVAPEGFAALLAEELERLEQAEPRGELPVAAAAAITSRRGFYEI
                     RAAHSPDTRLWCSRGSTAWTVVYEADPRFQLSCLNRFVYVKAVKDLSETLQGADPVRG
                     RVSTVGLAAPAAGMPALAAALARWGAARVCALGRMQQPPLAWRHDGRPALGDLVTWTD
                     WEQESLET"
     gene            13099..13473
                     /gene="queD"
                     /locus_tag="PKI07_09800"
     CDS             13099..13473
                     /gene="queD"
                     /locus_tag="PKI07_09800"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOC51383.1"
                     /translation="MELRKSFQFEAAHRLPRLPRSHKCRRLHGHSFKVEIAIAGPCDP
                     RLGWLMDYADISRAFKPIHDRLDHRYLNQVPGLANPTSENIAAWIWRRLKPRLPLLTE
                     IVIAETCSARCVYRGPDRPAAA"
     gene            complement(13580..15640)
                     /locus_tag="PKI07_09805"
     CDS             complement(13580..15640)
                     /locus_tag="PKI07_09805"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51384.1"
                     /translation="MKSHSGWGLLALVLALAGPVRGADPILIGQWPGWIFREANSVAV
                     AGNYAYVAADSGGLQVFDVSNPANPQRVGGYYIGVWAQGVAVSGNYAYVAATAEGLQV
                     IDVSNPANPQWVGSYDASGEAYGVTVSGNYAYVAAGDGGLQIIDVSNPANPQRVGGCE
                     TPGWALNVAVGSNYVYVADNDEGLQIIDVSNPANPQRVGGYATSGWVLGVAVSGTHAY
                     VADAITGLQIIDVSNPANPQWAGDYDTGGEAGDVAVSDNYAYVAAAFAGLQVIDVSNP
                     ANPQWVGSYDASEGALGTAVSSNYVYVADRKAGLLVIDVSNPANPQGAGSHGLSGDAQ
                     SVAVNGNHAYVVDSDVGLRVIDVSDPAHPQHKGQFRAGKYVADVAAKGNHAYLVEEYE
                     GLLVIDVSNPANPQQVGGCSTSGYAQGVAVSGDYAYVAAGTAGLLVIDVSNPADPQQV
                     GRYDTSGIARDVAVKNYYAYVADGNAGLQVIAVSNPANPQRVGVYDTSGSGLGVAVSG
                     NYAYVADGLDGLQVINISNPSNPQRVGGCSTSGYAMGVAVSGNYVCVTDGEAGLQVID
                     VSDPANPQRVGGYDTSGDARGVVVSGNGAYVADGKWGLAVFNLPWVLRPQITSITHAG
                     GAATVYYTNTLPGTNYTLEYCTNLSSGAWQPVGTQPAGGTSASQTDPAAGGTQRYYRV
                     YYQP"
     gene            complement(16120..>16643)
                     /locus_tag="PKI07_09810"
     CDS             complement(16120..>16643)
                     /locus_tag="PKI07_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51385.1"
                     /translation="ADGTAGLQVINVSNPANPQRVGGCATLENALRVVVNGNFAYVAD
                     GGGGLQVIDVSNPANPQRMGGHITAGHASGVAVSGNYAYVADDWWGLAVFNLPQAPRP
                     QITSITHASGAATVYYTNTLPGTNYTLEYCTNLSSGAWQPVGTQPAGGTSASQTDPAA
                     GGAQRYYRVSYQP"
BASE COUNT         2661 a         5680 c         5544 g         2758 t
ORIGIN      
        1 gcaaatccgc atgcgcgtcc acctgcaccg cgaccaggtc gcgcgcgccg cgcgcctgcg
       61 ccagcccgcg cgccacgccg gcggaaatcg aatgctcccc gcccagcacc accagcagct
      121 ccggcgcggg cttcccgcgc agcaggcccg tcaccgcgcc ggccacccgc tcgatcatcg
      181 cctccggcga ctggagctcc gcccgcagcg gcggcagcgt atgcaccccg aacgccaccg
      241 ccggctcgca gtcaaactcc cggtcgtatt gctccacttc gcgcgacgcg gcgaggatcg
      301 ccgccgggcc gcgccgcgtg ccgggtgcgt aagaagtcgt ggcatcatac ggcaccggca
      361 gaacccacgc gcgcgcgcgt tgacgttggg tcaattccgc cggcaagccg agaaaattat
      421 actcgccttg aaaccagtca ctctcactca tttgtcaggg tatagtgggc ggcggcgcgg
      481 gcgctggcaa gcctatatcg cattcagccg ccgccaaagt ttccgcgcgc atttccgcgc
      541 cgcgatggat tggagaaaaa acccgccgcc gtcaacggtc ttccgggcgc gggtccccgc
      601 gcgccggccg cgccgccgcc cgggcgcgcg cggctaatcg gccgcgccca ggcgctgctg
      661 cagttcctcg agggaaaccc ccttggtctc cggcaggaac cgggccacaa ggataaactg
      721 caggaccatc atcagagcga agaaaccaaa ggcataggcg ccggagcgcg cggcgaccac
      781 cgggaaggac cagctcaccc cggcgcacag cacccagatg aggaagcaga ccaccgccga
      841 gcccgaggcg cgcacggcgt tgggcagcag ctcgttgatg atcacccaca ccaccgcgcc
      901 ctgcgaatag gcgtgcgagc cgaccacgcc catcatggcc acgatgacaa tccacccctg
      961 cgcgtgggtg gcgaaaaccc acgccgccag cgcgtgggac aggacgaacg tgaccgaccc
     1021 gatcagcagc agcgccttgc gcccgatgcg gtcaatcagc gccatcgcca ggatcgtcat
     1081 cgccaggtta gtaaagccaa tgatcaccga ctgcgaaagc gcgctcgtct ggtcggcccc
     1141 cgccatgcgg aaaatatccg ccgtgtagta aatcacggca ttgatgccat cgagctgatt
     1201 gaaggcgcca atcatgcagg ccagcagcag cggcttgaga tattggcgct ggaacaggcg
     1261 cggccgcgcg ccggcgatct ccgctttcag cgagtccgcg atctcccggg cctccccggc
     1321 cgggtgctca tgcccgaaac gggccagcac cgcctcggcc tgcgcgcggc gtccccgctt
     1381 caccagccag cgcgggcttt ccgggatcag cagcgccgtc accagaaacg ccgccgccgg
     1441 ggcggtcatc acgccgaaca tccagcgcca ggcctgggga tgctccgcgc ccagcccgcg
     1501 cgccaccagg tagttggaga aataagccgc caggatgccc accacgatgt tcaactggct
     1561 gaccgccacc agccgccccc gcagccgggc cggcgcaatc tcggtgatat acatcgggca
     1621 gaccacggac gcgcccccca ccgccacgcc gcccagccag cggaagaaca gcagcgcatg
     1681 ccagttccac gccagcgcgc agcccacggc ggagagggcg aagaacaccg ccagcagcgc
     1741 cagcaccggc cgccgccccc accagtccgc cggccgcccc gccagcaacg ccccgatcat
     1801 ggtgccgatc aaggcgctgg agaccgtgaa ccccagcagg ttgtcattga gcgcatacac
     1861 ctgccgcagg gcctccgtgg tgccggagat caccgccgta tcaaaaccaa acagcagcga
     1921 ccccagggcg gccacaacgg cgctgagcaa cagcatgccg cggccgcggc gtccgcccgc
     1981 cgccgccctc gcccgctcct gactttgagt ttgcttcgcc atcaccggca cacccgtagc
     2041 gcggcccatt gttaatttgt tggccggagg aagtcaaccg gacaccggcc gcgcgggccg
     2101 gcccgccgcg ccccctcgcc cgccccgcgg cgcgccggga tgacgcccgc ggggcaagac
     2161 gccgctggct tttatccgcc gccggtttta tgctggccgg gtgaacctta tgagccacac
     2221 gcataatcgg tttggcgcgc tgcaaacatt tgacccgggg aatggccggc cgggagcgct
     2281 gtatgcgctg ccggagctgg aacgggccgg cgttggcccg gtttcccggc tgccggtgtc
     2341 cctccggctg gtgctggagt cggtattgcg gaattgcgac gggcggcggg tgaccgagac
     2401 gaacgtgcgg gcgctggcca actgggagcc ccacgcgccg cgccgcgagg agattccgtt
     2461 cgtggtcgcg cgcattgtgc tgcaggactt cacgggggtg ccgctgctgg tggacctggc
     2521 ggccatgcgc tcggccctgg cgcggctggg ccggaacccg ggcctcgtcg agccgctggt
     2581 gccggtggac ctcgtcgtgg atcattcggt gcaggtggat taccacagcg cgccggacgc
     2641 cctcgaaagg aatatggcgc tggagttcca gcgcaaccgc gagcgctacc agttcctcaa
     2701 gtggggcatg caggcgttta agaaattcaa agtggtgccg cccggcatcg gcatcgtcca
     2761 ccaggtaaac ctggaatacc tcgcccaggg ggtcgcgtcg gcgcccctcc ccggcggcgc
     2821 gcccgcgccg gccctgttct accccgacac gctggtcggc acggattccc acaccacgat
     2881 gattaacggg ctgggcatcg tgggctgggg cgtgggcggc atcgaggcgg aagccggcat
     2941 gctgggccag ccggtctatt tgctcacgcc ggatgtcgtc ggcgtgcacc tgttcggcga
     3001 actgcgcgag ggcgtcaccg cgacggacct ggcgctgacg gtgacccagc ggctgcggca
     3061 ggcgcgggtg gtggggaagt ttgtcgagtt cttcgggccg ggcgccgcgg cgctgccggt
     3121 ggtggaccgg gcaaccgtcg ccaacatggc cccggaatac ggggcgacga tgggcttctt
     3181 ccccattgac gccgagtgcg tccactacct gcgggcgacg ggccgcggcg aagaacactg
     3241 ccgcgcgtac gagaactact accgcgcgca gggcttgttt ggcatgccgc aaaagggcca
     3301 gatcgcctac acgcaggagc tggaactgga cctggccacg gtgacgccca gcgtggccgg
     3361 acccaaacgg ccgcaggacc ggattgaatt gcccaacctc aaacgcgagt tcgaggcggc
     3421 cctggcccgg ccggccgccg aaaacggctt tggccgcgcc gccgcggagc tggcgcagag
     3481 ccacccgctg ccacacggcg gcggcgccct gcgccacggc agcgtcatga ttgcgtccat
     3541 caccagttgc acgaacacga gcaacccctc ggtgatgctg gcggcgggat tgctggccca
     3601 aaaagcggtc gcgcgcgggc tgcgcgtccc cccgtgggtc aaggcttcgc tggcgcccgg
     3661 ctcgcgggtg gtcagcgatt acctgcgccg gacgggcctg cagcagcccc tggaccagct
     3721 cggcttcagc ctggtgggct acggctgcgc cacctgcatc ggcaactccg gcccgctgcc
     3781 ggcgggagcg gaggacctca tcagccggca tcacctcgtg gtggcggcgg tgctctccgg
     3841 caaccgcaac ttcgaggcgc gggtgcactc cagcctcaag gcaaacttcc tcatgtcgcc
     3901 gccgctggtg gtcgccctgg ccctggcggg gcgggtgaac atagacctga gcaccgaacc
     3961 gctgggccgg gacaaagacg gccgggaggt ctatttgaag gacatctggc cggcccggga
     4021 cgaagtgcgc gaagccatgc gcgccgcgct caagccggaa gtattccgcc agctctaccg
     4081 cgacctggcc gcccaggtcc cccagtggag ccaaatcccc gcccgcggcg gcgaagtgta
     4141 ccaatgggac cccgacagcc gctacatcca ggagccgccc ttcttcgcca acttcagcct
     4201 ccagcccggc gcgacctccg atctccgcgg cgcccgcgcc ctggccatct tcggcgacag
     4261 cgtgaccacc gatcacatct cgcccgccgg cagcatcaaa cccgcctcgc ccgccggaaa
     4321 atggctgctc gaacgaaacg tcaccgtcgc ggatttcaac agctacggct cccgccgggg
     4381 caatgaccgg gtcatgaccc gcggcacctt cgccaacgtg cgcatccgga acctgatggt
     4441 ccccggcgtc gagggcggcg tcacccggtg cttcgcgcgt tcggacaacc cgggtgcggc
     4501 gggcgaaccg atctcgattt acgaggcggc gatggcctac cagcgggaag gcacgccgct
     4561 cctcgttctg gccggccagg aatacggcac cggcagctcc cgcgactggg cggccaaagg
     4621 gccggccctg ctgggagtca aagccgtgat cgcgcagagc ttcgagcgga tccatcgctc
     4681 gaacctcgtc ggcatgggca tcctgccgct gcagttcaaa gacggaacca gcgcgcaaac
     4741 cctgaacctg gacggcacgg aaaccttcga catctccggc ctggcccacg ggcttaaacc
     4801 ccagcaggaa ctgaccgtgt ccgtgcggcg cgccaacggc cgggaggaag ccgtgccggt
     4861 catctgccgg atagatacgc ccatcgaact ggaatactac cagcacggcg ggattctgcc
     4921 ttatatcctg cggcaactgg ccggcgcctc gggccccgct cccgcctgat ccgatgggcc
     4981 aagagcggcc catcgccatc gggcaatcca accggaaagg ccccctgaaa cggccacgcc
     5041 cacgctggat acgagttttt tgacctttat actgactttt agccgttttc gtcaccgcca
     5101 gaatggagag aaagctggct gaaggccaac ccgagctgat tccatgagag ggtcaatcct
     5161 gtgccatcgt tccacccaaa ccggcccaat cgccccggcc atacccccaa cgaacccgga
     5221 taagggtaaa acaggcgtaa aaccctgatt tttccggctt cggcttaccc tgaacgctcc
     5281 ttgcttttac cctgcttcta cgctgtgtac aaggggtgta caagggatgc ccaccgggag
     5341 tccatggggt ttaagctggt gcatccgttg agcatccctt gtacacccct tgtacacggc
     5401 ctgaaggcag ggtattttca cggtatccgg gtagtggatg cgggggcttg aaaaggtcga
     5461 aacgaggaga gaggacgaga ggattatttc taagctccgt aaaaacagct agttaggaag
     5521 ccgaggctgg caaaaggggg ctttttagga tcggaagggc gttcggatgg tctttgggtt
     5581 cgcgaacgcc ttatctgaaa cgatgccgct ggatagtcca gccgtgcata atcgtcctat
     5641 tggagccaga gtggggctgg cagccaaggc caaagggcta acgaatcgct tgcttggggc
     5701 ttttggctat gaaatcaggg gtttcggctg aaagagggaa ggcgcaggcg gcccgtgcgc
     5761 ccgcgcgcgg tgcatgaggg ccgtttcctt tgagggacga acggcgcggg ttggctctcc
     5821 ggcggcgtcg gcgagcaagg gcggggctgg cattggccgg gggcgcttaa aaactgaggg
     5881 gcgcttgaaa ttatgcgtgc ggcacggcgg cgttggtggt aaaagttcgt cggcggagtc
     5941 catgattatg acgaccagta aaccaggcga aactgtggag ccgggcgcgc tcgggcgcgt
     6001 gacctttctt gatggcaagg gcgatctgcc gatgctggaa atcagcaccc gctggagcgc
     6061 ggcggagatc tatctgcatg gggctcacgt cacccacttc cagaagaagg ccgagccgcc
     6121 gatgctgttt ttgagccaat gcagccggtt tgccgagggg cagccgattc gcggcggcat
     6181 tcccgtgatt ttcccctggt tcgggcggcg cgagggcctg ggccagcatg gctttgcccg
     6241 cctcaagacc tgggaactga aagaggtggt tcccgcgccc gacggcagcg tcagcgtgcg
     6301 gctgcggctg cccgacggcc cggaggcggc cgggtttccc cggttcacgg cggaatacat
     6361 cgtcacggtc aaccaggcgc tgacgctgca gctcgttgtg gccaacccgg cccgggatgc
     6421 cgagtttacc ttcgagaact gcctccattc ctactttgag gtgggcgaca tcgccaccgc
     6481 ttccgtggtc gggctcaagg ggcttgccta tgaggatcgg gtgggcgctt gcgtggcggg
     6541 gcgcgagtcg gccgacgcgc tgcgcgttgc cgcggaagtg gatcgcattt accaggacag
     6601 caccgggccg ctggagattc tcgacccgtc gctgggccgg aagattcgca ttgaaaaaca
     6661 aggctccgcc tcgacggtgg tgtggaatcc gtgggttgcc aagtcccggc agttggcgga
     6721 tttcggggat gaggaatacc ggcgcatgat ttgcgtggaa tcgggcaacg tcggcccggc
     6781 cagcattcgc ctgctgccgg gagccgcgtc cacccttacg gtgaagttga gcacggagcc
     6841 gctcaaataa cccgcgcccg ggccggttgg ttccgccgcc ggcagcggcg cggggccggc
     6901 gcccggcgcc cgccctgcgg cgggggggcc gggcctgatt ccggcccgga gccgtggtag
     6961 agattatgcc tctggaatac aaagattatt acgagaccct gggcgtgccg cgaaacgcca
     7021 gcgaagcgga tattcgcaag gcttttcgca agctggcgcg cgagtatcat ccggacgtcg
     7081 ccaagaacaa gaagcaggcg gaggagaagt tcaaaacgat caacgaggct tacgaagtgc
     7141 tgggcgatcc ggccaaacgc aagcgctacg atgaattggg ggcgaactgg cgggaggggg
     7201 ccaacttccg cccgccgccc ggctggcaat cattcgggcg gggccgcgcg gccggcggcg
     7261 gccccggcgg ggaagcccac gagtttcgct tcagcggcac cggcttcagc gatttcttcg
     7321 agcagttgtt cggcgcgcgc gcggcgcggg gcgcgtccgg cttcgggcac ggcggcgggt
     7381 ggggggcgga agaaatgggc gccgagcgcg ggcaggatat cgagggcgac atcctggtga
     7441 cgctcgagga agccatgcgc ggcgcggtgc gttcggttgc cgtgcagcgc gccgttccct
     7501 gcggccaatg cggcggcctt gggcatcgcc agcgccgcgc ctgcgccgcc tgcggcggca
     7561 ccggccaggt ggtgaagacc gatacgtggc aggtgaaaat ccccgcgggc gtcacggaag
     7621 gccagcggct gcggctggcc gggcgcgggg aggcgggcgt cggcggcggc gggtcgggcg
     7681 atctttactt gcgcgtgcgg ctggcccggc atccggattt cagggtggac gaccacaacc
     7741 tgatctacgc ggcggaagtc gccccgtggg aagcggtgct cggcgcgagc ataaccgttc
     7801 cgacgttggg gggaacggtg cgcatcaaag tgccgccggg gacacagaac gggcagaagc
     7861 tgcgcgtgcg ggggcgcggg ctgccccggc gcggggggcc gagcggcgat ttgattgtgg
     7921 aaatccgcat cgaggtgccc cagcaggtct ccagcgcgga acgcaagctg tgggagcaac
     7981 tggcgagcga gtcgcggttt aacccgcggc aataaccgtc cggcggagcg gcttccggcg
     8041 cgcgcccgcc gtccgccgcg cggaccgggg ggcgcgcggt tgcgcccgcg gggttcccgc
     8101 gcccgggcgc gcctttcggc gccgggcctt gggccggtca ggccgttgcg ggccgggcca
     8161 gctttctcca gatcccggct cccaggggaa gcagcaacag cagcgacaga tccaggaacg
     8221 tcgtcgcctc cggcacggac tgggacgggc ggtacgtgta ttggacttca accgcggccc
     8281 cggccacggt gttgagccgc gcccaaatgt ttccgccgcc gccgagaata atctcctgcg
     8341 tttgcgtcgt ccccggcagt gtgaacgtgc ccgtgccgat aaaccagtcc aggtcctggg
     8401 gccgcgtgta ggcatagctg acggtgtccg tggagctgac ggggccatag tcggcgactc
     8461 cgccgctggg caactggtaa acctcggccg tggccagggg cgacggctgc agaaaaccaa
     8521 agccgcccgg caagtccacc gtcagcaggg agccggcccg gagggagaag ttttgcgggc
     8581 gggtggaggt gttccggatc gtgccggtca tgttcatcgt ctggctggcc aggatattca
     8641 ctgactccag cgtgcccagg gccgggttga attgcggcaa acagaaggtg tggctccaat
     8701 ccgtggtggt ctcggggaaa acgtccacga aagccgtcac gaccgtttgc gacagggaag
     8761 acatcacgat ggcctgggcg gcatttgccc ccagcagcaa tcccgcgccc gccagtagaa
     8821 gtatcggcct gcatttagtt ttaaagttca tggcccaatc ctaacggcgg aggcgcatcc
     8881 gttctatcgg gcgggggctg gttttcacta agggaatcgc ccggatttcg ccccgggcat
     8941 ttcccgggcg gggcggcggc ttggcgggag ggggattgcg tttggcagcg cgtatgggaa
     9001 tcgaacccat ctttcagcct tgagagggcc gtgtcctaac cgatagacga acgcgccgcg
     9061 ccgccaatct caggcatagt aaccgttggc ggcgggatgt caatgcggcg ttgggaaaca
     9121 ttcgcggccc gcgcgccggc ggcccgctgg cgcggcgcgg cgcgcgggtc cgggtggggc
     9181 cgtttcccga cttgcgggcc gccgccgccg cgggtatagg ttatttccgg gctgtggcgg
     9241 aaaaccagcc tggcgaaaac aaaaccgcat ggattccaac cgcgagttag cgagctttgc
     9301 cgcgcgtttg catcgggaaa ttggccccgg ccaggattct ccgaagcaga cgcgggcggc
     9361 gtggccggcc tttaatccgc tcgcgctcga gctgttcgcc ctccaattcg cccacaaccc
     9421 gctctaccgc cggttctgcg aagcccgcgg agcgcgcccc ggcacggtgg cggactggac
     9481 ggaaatcccc gccattccca cggcggcctt caaggcgggg ccgctttcgt gtttgccggc
     9541 ggcggcgcgg acggcggttt ttcattccag cggcacgacc gggcagacgc cgagccggca
     9601 ttttcacagc gcggattcgc tggcgcttta tgaggcgtcg ctccgggcgt ggtttgacgc
     9661 ccattggccg gtggcggcgg gcccgtgggc ctgggtgatc ctcaccccgc cgcccgatca
     9721 agctccccat tcctcgctgg ttcacatgtt cgagacgctg cgccgcgccc ggaagcccgc
     9781 cgccgcggcc ttcgtcgggc gggcggcgga agacggcgcc tgggtgctgg acgtggacgc
     9841 cgcgtggccg cttctgcagt cggcggccgc cggtgcgcag cccgtgtttc tgctgggcac
     9901 ggccttttcc tttgtgcatc tgctggatga ttttgccgcg cggggccggg gactgacgct
     9961 gccggcgggg tcgtgcgcgc tggaaaccgg cggctacaaa ggccgctcgc gcgcgctgcc
    10021 caaggcggag ctgcacgcgc tgatgtcccg gcgcctgggc ctccggcccg accgcatcat
    10081 ctgcgaatac ggcatgagcg aattgagttc gcaggcgtat gacggggagg tgggcccggt
    10141 tgcggcgcgg gcgcttcccg ggctggcgtc ccccggcgcg cggcgcttcc ggtttccgcc
    10201 ctgggcgcgg gtgcggatta tttcgcccga gaccggcgcg gccgtggagg agggcgaaac
    10261 cgggttaatc tgcgtgttcg acctggcgaa tgtttactcg gtgctggcgg tgcagaccga
    10321 ggacctgggc cggcgccgcg gttcgggctt tgagctggcg ggccgggcgg cgctggccga
    10381 gccgcgcggg tgctcgctca tggcggttta ggagccggga atgaacgcgc gggaacacgc
    10441 tcaacaccgg gcccggacgc tgtggctgac gggggtgctg catgccttta cgcatcttta
    10501 ccaggcggtg ctgctgccgc tttacttgtt gatccagaag gattttcagc tcgccagcgt
    10561 ggggcgggtg actctgctgg tgacggtgat gctggcggcc tgcttcgggc cgagctaccc
    10621 cgccgggatt ctggcggacc gcctgaatcg caagaagctg ctggccttcg ggctggcgct
    10681 caacggcacg gcgtttgcgg cgctgggcct ggcgcccagt tatgcctggg cgctggtggc
    10741 cgtggtggtg gccggcatcg gcagcagttt ttatcatccc gcggccacgg ccatgattac
    10801 gcgcctgttt ccgggccgca ccggcaaggc gctggggctg gtgggcatcg gctcgagcgt
    10861 gggcttcttt atggggccgc tttacgcggg ctggcgcgcg cagatgctcg agccggtgct
    10921 ggggccggcg gcctggcgcc gcccggtgct cgagctgggc cttttgggcg tcatcaccgc
    10981 ggggttgttc tattggctgg cggatgagga gcagccctcc gcgcccgccg agcgcaaggc
    11041 cgttccgtcc ggccggatgt ttcccacgcc ggccctgtgg tttttcttct ttgcgtattg
    11101 cctggccttt agcttccggg atttcgccgg ggcgagcatg ggcagcctga gctcgctctt
    11161 tctgcaaaag gcccgcggct tcaccccgca aagcaccggc gtggccctga gcctcctctg
    11221 tatcgcctcc gtcgtcagca atccactgtt tggccacctg agcgacgggg gccgccgccg
    11281 ctggattacc ctggtgttaa ccatcgccgc cgtgctggtc gccgccttcc cgcatgtccc
    11341 gccggcctgg accctgccgg tgctgatcgt gtatggcttt ttcttcatgg ccagctatcc
    11401 catgaccgag gcggcgctca tggaatcggt gccggatgcc gtgcgcgggc gggtctttgg
    11461 cgtgttcgtc acggccgggg gcgtgattgg caacctctcc cactggctgg tcggcgccaa
    11521 gataaggcac ctcggcgcgg cggcgcaaaa cgtcagcgcc tacttcccca tctacggctt
    11581 cgtgggggtc ttgatcctgg tcgccctttc cgggttattg tgtctcgggc cgattcgacg
    11641 ccgcgagggg ccgctgccgc tcgcgacggc gcgcgcgccg tccctgccgc cgcagacatg
    11701 aacctgccca attatttcct ggccgacctg ccgcccgagg cggcgttgac ccccgcgctg
    11761 attgacgaag cctgccaaac gctgcggcgc aaccgcgaat gctacctggc ccaccggtcc
    11821 acgcagagcc tggtccaggt cttatgccgc gtagcccggg actggctgga accgtccgac
    11881 ccgtttcgcc aattcgccct cgaacaaagc ccggcggcca ccggcttccc gcgcgccacc
    11941 ctggcccgcg gcctggacgc ctttttcaag tccctgaccc ccgagcattt ccacgcgctg
    12001 ctcgaacaag acctgggcca cgttcgccgc ctggacgaac tgacgtccgc cgctcccgag
    12061 cgggccgcgc gccgcgccgc cctggcgacc gcgccggagc tgctggcgca tatcaccgcc
    12121 ggcaacctgc ccagcccggc gctggcggcg atcgtgctgg gcgtgctgac gcgctcggcg
    12181 cagttcgtga aatgcgcccg cggcgcctcc ctgctgccgc gcctcttcgc gcactcgctg
    12241 taccaggccg agcctaaact gggcgcgtgc ctcgaattgg ccgagtgggc cggggggcgg
    12301 caggacttgg aagcgccgct ctttgcggcg gcggattgca ttaccgccac cggcaccgat
    12361 gcgacgctgg ccgccatccg cccgcgcgtg cccgccgcgg cccggtttct ggcctacggc
    12421 caccgtgtca gctttgccta tgtggccgcc gcgggcctgg ccggccggca agcgcggcag
    12481 atcgcggcgc aagccgcggc ggacgtggcc gcctggaacc agcagggctg cctttccccg
    12541 cacgtcattt acgttgagct gggcggcggc gtggcgcccg agggcttcgc ggcgctgctg
    12601 gccgaggaac tggaacggct cgaacaggcc gagccccgcg gcgaactgcc ggtggccgcc
    12661 gccgccgcca ttacctcgcg ccgcggcttc tatgaaatcc gcgccgccca ttcgcccgat
    12721 acgcgcctct ggtgcagccg cggctcgacc gcctggacgg tggtttatga agccgacccg
    12781 cgctttcaac tctcctgcct caaccgcttt gtctatgtca aagccgtcaa agacctgtcc
    12841 gagacgctgc aaggcgctga cccggtgcgc ggccgggttt cgacggtcgg cctggcggcg
    12901 cccgccgccg ggatgccggc gctggcggcc gcgctcgcgc gctggggcgc cgcccgcgtc
    12961 tgcgccctgg gccggatgca gcaaccgccg ctggcctggc gtcacgacgg ccgccccgcc
    13021 ctgggcgacc tggtcacctg gaccgattgg gaacaggagt ccctggagac ttaaatgaaa
    13081 tccccacgcc tcatgcaaat ggagctgcgc aaaagctttc agttcgaagc ggcccaccgg
    13141 ctgccgcgcc tgccccggtc ccataaatgc cggcggctgc atggccacag cttcaaggtg
    13201 gaaatcgcca tcgccggccc ttgcgatccc cggctcggct ggctgatgga ctacgccgac
    13261 atctcgcgcg ccttcaaacc catccacgac cggctcgatc accggtacct gaaccaggtt
    13321 ccggggctgg ccaatcccac cagcgagaac atcgccgcct ggatttggcg ccgtctcaaa
    13381 ccccgcctgc cgctcctgac cgagatcgtc atcgccgaga cttgctccgc gcgctgcgtc
    13441 tatcgtggcc cggatcgccc cgcggccgcg taaatcctcc cgccgcgcgc ggcgccctcc
    13501 agcggcttgc ggccgcttgc ccgttcaggc ctcgggaacc cggcgggcgc gccgggttcg
    13561 cagagaggaa acgctccggc taaggctgat aatacacgcg gtagtagcgc tgggtgcccc
    13621 cggccgccgg gtcggtttga gaagcgctcg tgccgcccgc cggctgcgtg cccaccggct
    13681 gccaggcgcc ggagctgaga ttggtgcagt actccagggt gtaattcgtg cccggcagcg
    13741 tgttggtgta atacaccgtg gcggccccgc cggcgtgggt gatggaggta atctgcgggc
    13801 ggagaaccca gggcaggtta aagaccgcca ggccccactt tccatccgcc acataggcgc
    13861 cgttgccgct gaccaccacg ccccgggcat ccccgctggt gtcgtagcca cccacccgct
    13921 gggggttggc cggatcgctc acgtcaatca cctgcaagcc cgcttctcca tccgtcacgc
    13981 agacgtagtt gccgctgacc gccaccccca tggcataccc gctggtgctg cagccgccca
    14041 cccgctgggg gttggacggg ttgcttatgt tgatcacctg cagcccatca agcccatccg
    14101 ccacgtaggc atagttgccg ctgaccgcca cgcccaggcc gctcccgctg gtgtcataga
    14161 cgcctacccg ttgcgggttg gcgggattgg acacagcaat tacctgcaag cccgcattcc
    14221 catccgcgac gtaggcgtag tagttcttca ccgccacgtc ccgggcaatc ccgctagtgt
    14281 cgtaacggcc cacctgctgg gggtcggctg ggttgctcac atcaatgacc agcaaccccg
    14341 cagttccagc cgcgacgtag gcgtagtcgc cgctcaccgc cacgccctgg gcatacccgc
    14401 tggtgctgca gccgcccacc tgctgggggt tggccgggtt gctcacgtca atcaccagca
    14461 aaccctcata ttcttccacc aggtaagcat ggtttccctt cgccgccacg tcggcgacat
    14521 acttgcccgc gcggaactgg cccttgtgtt gcggatgggc cgggtcgctg acatcaatca
    14581 cccgcaagcc cacatcgcta tccaccacgt aggcgtggtt gccgttcacg gccacgcttt
    14641 gggcgtcccc gctgagtccg tggctgcccg caccctgggg gttggccggg ttgctcacgt
    14701 caatcaccag caaacccgct ttcctgtccg ccacgtagac atagttgctg ctcaccgccg
    14761 tgcccaaggc gccttcgctg gcatcgtagc tgcccaccca ttgcgggttg gcgggattgc
    14821 tcacgtcaat cacctgcaat cctgcgaagg ctgccgccac ataagcgtag ttgtcgctga
    14881 ccgccacgtc cccagcctct ccgccggtgt cgtagtcgcc tgcccactga ggattggcgg
    14941 ggttgctcac atcaattatc tgcaaacccg taatggcatc cgccacgtag gcatgggtgc
    15001 cgctcaccgc cacccccaag acccatccgg aggtggcgta gccgcccacc cgctgggggt
    15061 tggcggggtt gctcacatca attatttgca gcccctcatc gttatccgcc acatagacat
    15121 agttgctgcc caccgccacg ttcagggccc acccgggggt ctcgcaaccg cccacccgct
    15181 gggggttggc gggattgctc acatcaatta tttgcagccc cccatcgcca gccgccacgt
    15241 aggcgtagtt gccgctgacc gtcacgccat aggcttcccc gctggcatcg tagctgccca
    15301 cccattgcgg gttggcggga ttgctcacgt caatcacctg caatccttcg gcggttgccg
    15361 ccacataggc gtagttgccg ctgaccgcca cgccctgggc ccacacgccg atatagtaac
    15421 cgcccacccg ctgcgggttg gccgggttgc ttacgtcgaa cacctgcaat ccgccggagt
    15481 ctgccgccac gtaggcgtag tttcccgcca ccgccacact attggcttcc ctgaagatcc
    15541 aacccggcca ttggccaatt aaaatgggat ccgccccccg aaccggcccg gccagggcca
    15601 gcaccaaagc taacagcccc cagcccgagt gacttttcat aaaccgcatg gattgcttgt
    15661 gtacgttcat tgtgttttct tttctgatca tcattgactc tgtgtggcac cgttctccgc
    15721 gcgtcaaacg gaaggcggca taacgcagcg cccacgcttg ctttttacca ccccgtccgt
    15781 cccgcgcgcg gataaatatc tctaacgctt ctgtccaccc ggacgaagtc gagattatga
    15841 aaacagaccg cgcatgtcaa tttctgattt cgccggattg agcggattcc gggccgccgc
    15901 agcaaagaaa cccagcgctc ggaagaggag ccaggctcag tgcgcgccag cccttccggc
    15961 atccctgcgg ggaagcggag ctttgagggc caatgacccc gagccaaact gggtcacgca
    16021 aatccccagc gcggagagtc cccgcccgcg gacagcttgc ccgtttaggc ctcgggaacc
    16081 cggcgggcgc gccgggttcg cagagaggaa acgccctggc taaggctgat acgacacgcg
    16141 gtagtagcgc tgggcgcccc cggccgccgg gtcggtttgg gaagcgctcg tgccgcccgc
    16201 cggctgcgtg cccaccggct gccaggcgcc ggagctgaga ttggtgcaat actccagggt
    16261 gtaattcgtg cctggcagcg tgttggtgta atacaccgtg gcggccccgc tggcgtgggt
    16321 gatggaggta atctgcgggc ggggagcctg gggcagattg aagacggcca gaccccacca
    16381 gtcatccgcc acataggcgt agttgccgct gaccgccacg cccgaagcgt gcccggcggt
    16441 gatgtgtccg cccattcgct gcgggttggc ggggttgctc acgtcaatca cctgcaagcc
    16501 tccccctcca tcggctacat aagcgaagtt gccattcacc accacgcgca aggcattttc
    16561 gagggtggca caccctccca cccgttgcgg gttggcgggg ttgctcacat taatcacctg
    16621 caaacccgca gtgccatccg cca
//
LOCUS       DAOPQG010000081        16173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4287_length_16173_cov_14.845080, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000081 DAOPQG010000000
VERSION     DAOPQG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 16173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16173
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4287_length_16173_cov_14.845080"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..635
                     /locus_tag="PKI07_09815"
     CDS             117..635
                     /locus_tag="PKI07_09815"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC51386.1"
                     /translation="MLKLVILSAGMAGRACELTAEKTTIGRSDDNTFPIAEPSVSSHH
                     CEVLLRGKDVVVRDLDSTNGTYIGPERVAPEKVLRPGEILRLGQVQMRLEAAAAAVPG
                     KKHFDRTTIIPGGVKASELDYTHVGPGFDTKSAGFARKDNPVNRIFIVGGIIFGLVIL
                     ALLLYIATMIKK"
     gene            complement(1114..1680)
                     /gene="def"
                     /locus_tag="PKI07_09820"
     CDS             complement(1114..1680)
                     /gene="def"
                     /locus_tag="PKI07_09820"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOC51387.1"
                     /translation="MPLVVVKYGTPVLRQKGERIESITPAIRQLAADMLETMYVKRGI
                     GLAAQQVGVATQLAVIDVRGVTDRPSSLERHGKSANVDAFMPLVLINPELNPLGEPES
                     GPEGCLSFPEIYADITRPAGVEVKALDLEGKPIEFRCGGLLARVIQHEWDHLQGILFI
                     DRMDRATKASLKPELDLLMAETKAALKL"
     gene            complement(1683..2786)
                     /locus_tag="PKI07_09825"
     CDS             complement(1683..2786)
                     /locus_tag="PKI07_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51388.1"
                     /translation="MREQTATQLLNAGSRVGRMTAFIGLDGFVDDILHVVDKRESAEK
                     YTRLPTIAQLAERLAGAAGRSTNIELVSQLNKLGGNGPIMANALASFGLKVTYLGILG
                     YPNLHPVFADFARRAEVHSIAEPGYTDALEFEDGKIMLGKHQSLRQMNWENIKSRFGL
                     DKFNASFGSADLVGFVNWTMLTHMSDIWAAVLKEVCPGLKGPRRKLFVDLADPEKRPP
                     ADIRSALQLITAFQQYFDVVLGLNEKEGFEIGKALGLNPSDHSQEGLLKLCQDIHQRV
                     PVDTIIVHPTAYALASGPEGTAIVQGPYTPKPKITTGAGDHFNSGFCLGKLLGFPTER
                     CLLTGVTTSGFYVRTGQSPAIADLAEMLRHWPA"
     gene            complement(3004..3738)
                     /locus_tag="PKI07_09830"
                     /pseudo
     CDS             complement(3004..3738)
                     /locus_tag="PKI07_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            complement(4091..4879)
                     /locus_tag="PKI07_09835"
     CDS             complement(4091..4879)
                     /locus_tag="PKI07_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOC51389.1"
                     /translation="MEDLIARMFNLTSWIVPLIILGAIVLPQAVRVLREYERGVIFRL
                     GKLHARGAKGPGLIFLIPVVDRMVKMDLRVVTIDVARQEVMTRDNVPVTVDAVVYFRI
                     VQPVDAVVKVENFYKATSLIAQTTLRSVLGQSPLDDLLSQREAINLKLQEIIDRQTEP
                     WGVKVTAVEVKDVALPDSMKRAMAKQAEAERERRAKIVNAEGEYQAAEKMVQAASMIS
                     QEPIALQLRFLQTMREISSEHNTTTFVPIPIDLFAPFMKGPPKA"
     gene            complement(4904..6193)
                     /locus_tag="PKI07_09840"
     CDS             complement(4904..6193)
                     /locus_tag="PKI07_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOC51390.1"
                     /translation="MNRSRLILFGVLSCLAALAAPGAEVGLIKINGAIGPATASYITR
                     AIEAAAERENRCLIIQLDTPGGSLESTKRIVQAFYASPVPTVVYVAPEGAWAGSAGCF
                     ITLAADVAAMAPATSIGAAHPVSIGPGGEEKTSEVMKEKQEQITGSFIEGIANRRGRN
                     AEWARASVVESKAITADQALELHVIDLVAKDLPDLLRQLDEHEVNGKPLETADANVVE
                     IPRTTQERLLQLLSHPELMMILMLVAIYGIIGELSNPGAILPGVVGAIALILVLYMAT
                     ILPINSAGLALIGLSIVLFIMDIFAPSHGILTFGGIVSFLLGALMLFNRADPVFRLSL
                     AYIIPATLVTAAFFIFVVGSGLRAQWLPVRVGREALLGRTAPARERIDAAGGRVFIEG
                     EYWNAVSEVPIEPGQPVEIIAVHGLTLKVVPKQPPIR"
     gene            complement(6381..8936)
                     /locus_tag="PKI07_09845"
     CDS             complement(6381..8936)
                     /locus_tag="PKI07_09845"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC51391.1"
                     /translation="MPTDSSSADPLPAIPPGVTLTTLENGLTIIVREDHSAPVVSAQA
                     WCMAGSIHEGRWLGAGLSHVLEHMLFKGTTTRPGSRIDQEVQEAGGYMNAYTSFDRTV
                     YHIDAPNTGAPVALDVLCDVMQNATLPPDELAREQQVILREMDMNTDDPARRASRRLF
                     ETVYTRSPYRFTVIGYPDIFNELKPEDIRGYYHEKYAPNNVFYVVVGDVKPGEVVAQI
                     GKAYAKTKARALPPVVLPVEPKQAAPREICEEAPIELGHVHLAWHIPELLHPDVPALD
                     VLAALLGHGRSSRLYQSVREKQGLAHSVEAWTYSPGLPGLFGLSAVVDADKFTAARAA
                     LLAEVEQMKAAPVPAAELAKAVKQFVSATLSARKTMQGQAQDLGGSWLVANDLNFSQR
                     YLAAVQRVNPADLQRVAREYLTPENRTLYSLLPAGAAPRPAITSETSCDYPVQKFDLP
                     NGLRLLVKEDHRLPFVEFRAVFRGGVLAETPETNGLTQLTGKLLLKGTARRSAEDIAR
                     EIESVGGSLNSYGGNNSFGVNAEVLNSDFPAGLDLLVDVLLNPTFPAPALEREREVQL
                     AAVRDRKDHLLQSALLAMRRALFGETGYGLDALGAERSLRRLQVADLRAFHERFARPN
                     NCVLAIYGDVQTAPVRAAVERAFAAWKAAPLPALNSGPRALDALQRVTETRDKKQAVL
                     VIGYRGVTLQDADRYPLDLLHEACSDLGSRLFLRIREQLGLAYYVGALNVVGLAPGYF
                     AFYAGTAPDQAARVEAELLKEAELLRAEGLTAEELRRAKAKIIGQRKIARQDLGDFAT
                     ATALDELYGLGHAYTETEDARYEAVTLEQLQAVAGKYLRPEALVISLVQPARP"
     gene            complement(8949..9368)
                     /locus_tag="PKI07_09850"
     CDS             complement(8949..9368)
                     /locus_tag="PKI07_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51392.1"
                     /translation="MQEVSFEQALDAIQAKGPRYHREAYLFVREALEHTQKSIAQSSR
                     GRVRHVTGQELLEGIRTFALQQFGPMAKTVLEEWGVHRCEDFGVIVFNMVAVGWLAKT
                     SKDRRSDFAGGYDFNEAFVKPFLPKSKQAALAPAAKS"
     gene            complement(9533..9976)
                     /locus_tag="PKI07_09855"
     CDS             complement(9533..9976)
                     /locus_tag="PKI07_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016868059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOC51393.1"
                     /translation="MVYQDEISVSTTGPGDLHDLTEAVRRIVGASRVRRGIVHLFNVG
                     STGALGTIELEPGLREDLPALLNRLIPPGRNYGHERAWHDGNAHSHLQATLLGPSLTV
                     PIRDGELVLGTWQQVFHLECDVRPRRRTVAVTVMGDGPDGVRAGD"
     gene            complement(10007..12835)
                     /gene="leuS"
                     /locus_tag="PKI07_09860"
     CDS             complement(10007..12835)
                     /gene="leuS"
                     /locus_tag="PKI07_09860"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOC51394.1"
                     /translation="MAIGRRQYPFHLIEPKWQRQWDEQQTFRAWNPGETVPAGHPFAW
                     RHAGAGPERLPKFYILDMFPYPSGAGLHVGHPEGYTATDILARYRRALGYNVLHPIGW
                     DAFGLPAEQYAIKTGQHPRRTTEENIATFKRQIKSLGFSYDWSREVDTTEPEYFRWTQ
                     WIFLQLYHSWFNPQSNKAEPIATLPYPPELQSGAGLPAAELESKRRAYRDAKRLAYVT
                     EAPVWWCEQLGTVLANEEVVDGKSEVGGFPVTRKPMRQWMLRITAYAERLLEGLETID
                     WSDSLKEMQRNWIGRSEGAEVDFRIVGAPGVIRVFTTRPDTLFGATYMVLAPEHKLVD
                     ALTSPAQRPQVEAYRAAVAQKSDLERTELAREKTGVFTGGYARNPVNGQEIPVWIADY
                     VLLSYGTGAIMAVPGHDTRDFEFASRFKLPIVQVVEPPAGVDWRGFVEDGISVNSTGA
                     DISISGLPTPEAKRKITAWLEAKGWGKPTVNYKLRDWLFSRQRYWGEPFPILWKTGPD
                     GRAYHEALPESALPLMPPPLEDYKPTPDGQPPLARAREWLNLPEGATRETNTMPQWAG
                     SCWYYLRYLDARNRAAFCAPEAERYWMGTSAPAAAPAAAPSAPGVDLYVGGTEHAVLH
                     LLYARFWHKVLYDLGHVSTPEPFFKLVNQGLILGEDGQKMSKSRGNVINPDDILAEYG
                     ADAFRLYEMFMGPLEMVKPWSTKGVEGVYRFLGRVWRLFVDEQSETEFEQALTAEPQR
                     GRELLAVIRRAPGIADCPPTAAQLKVLHACIKKVTEDLDGLRFNTAIAALMVFINEAM
                     TWEAKPVAVLRDFLVLLHPFAPHLAEELWSLLFAAGVSESGPARSLPAGGPSPAGGAA
                     GAPAVPPLSYAPWPKFDPALLVEDTLEIPVQINGKLRDVITVPANASQADLEAAARAS
                     GKVKPFLEGKTVRKVIVLPKKLVNIVAA"
     gene            13016..15394
                     /locus_tag="PKI07_09865"
     CDS             13016..15394
                     /locus_tag="PKI07_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51395.1"
                     /translation="MDKPPRHSTRPGRAVADQTPFSRLHRKGLMALICAGLAALGGAG
                     GGFAAETLLPAGDAPAPLISRHFPDRLHEFVWRNWQAVEPARLAKIVGASERDITALA
                     ASMGLPPAAQVPRALRERGYITLLRRNWHLLPYEQLLELLELTPERLAFMLREEDFLW
                     IKLGQLKPRCAPLGYQPPDEAARRHAAEIRRVVEADFGAEIRRPAEPRFDFVRQLART
                     QGAPPRKPAPAESDSLRLVYSYVAVYGDPLLNPRLNPYPDGLLQRLSAAGINGVWLHA
                     VLRDLAPGGPAFPEFGVHHEQRLANLQTLVKRARQYGIGVYLYLNEPRAMPAAFFAQR
                     PDLAGVREDDATALCTSHPAVRQWMEDALAFVFARVPGLAGVYAITASENLTSCASHG
                     NWRSCQRCQARSDADIISEVAAVLEAGVHRGNPAARVLLSDWGWRGHGRAPDIIARLP
                     RSAWLMSVSEWDLPIERGGIPSTVGEYSISSVGPGPRALAHWQAARQAGLKIGAEIQF
                     NNTCEIASLPCLPVLDLVAAHAHKLVSATQLNAQLIGWTMGGYPSPNLQLAARLSRSP
                     APSPETALDELARERFGRAGAAHARKAWSLMSDAFRQYPFHIRVVYNSPVQYGPANAL
                     YPVKTGYRATMWGFPYDDLEGWRGPYPPEIFAAQFEKIAAGWQPGIAELRQAVAKAPA
                     RRRAEARADLRLAQAAAIHFQSVANQARFILARDALARPPAALPAEDRRRLRTEAARC
                     LESEIALARQLYTLTRQDSRIGFEPSCQYFYLPLDLVEKVIACRWLLEQFAQ"
BASE COUNT         2758 a         5469 c         5110 g         2836 t
ORIGIN      
        1 cgcgtctgaa tggggttcag aaggtcgtga gttcaaatct caccgccccg accatccttt
       61 cccggctcac gggggtgagc cgggtgcgga acccaaaggt gtgagccgga tgatttatgc
      121 tgaagcttgt gatcctcagt gcgggaatgg cggggcgggc ttgtgaatta acggcggaga
      181 agacgacgat tggccggtcg gacgacaaca ccttccccat cgcggagccc tcggtgtcca
      241 gccaccattg tgaagtgctg ctccgcggca aggacgtggt ggttcgcgac ctggattcca
      301 ccaatggcac ctacatcggc cccgagcggg tggccccgga gaaagttctc cgccccggtg
      361 agattctgcg gctcggacag gttcagatgc gcctcgaagc agcggcggcg gcggtgccgg
      421 gcaaaaagca ctttgaccgc accaccatta tcccgggcgg cgtaaaggcg agcgagctcg
      481 attacacgca tgtgggcccg gggttcgata cgaaaagtgc cggcttcgcc aggaaagaca
      541 acccggttaa ccgcatcttc atcgtggggg ggatcatctt cggcctggtg atcctggccc
      601 tgctccttta catcgccacg atgattaaaa agtagccgcc gcccgccggc ccggaatccg
      661 cggagttcct ggcggtgggg ggactcgtcc ggcgctagcg cgagcggcgc acggaacgct
      721 tttgggggcg ggcaggtttg ggagtctggc ggcggacttg ccgctggatt tttacgatct
      781 ggctggccag gttcgtttcg acgtgttccc tccatataaa acggcaggga cagtccttgt
      841 tcggacactt ggccaggcgc cgtaattgct ttcgtcgttc acgaactaat gcggccagct
      901 tggttaactc agcttccaac gtggatgccc gcggcggttt cccattatgt ttcatgaatc
      961 ttaggctcta ttctaacctg tcggacgata attgtcatct gaatacccgc attccggccc
     1021 ggagatgggc cgggcgggga cgcgggcgga acgccagttc ggaacgaaag catggccgga
     1081 ccaccggccg cgccgggggg cggcccgatt ccgctacaac ttgagggcgg cctttgtctc
     1141 cgccataagg agatccaatt cgggtttgag gctcgccttg gtcgcccggt ccattcggtc
     1201 tatgaaaaga atgccctgca ggtgatccca ttcatgctgg atgacgcggg cgagcaggcc
     1261 cccgcaacgg aattcgatgg gcttgccctc caaatcgagc gccttgacct ccacccccgc
     1321 ggggcgcgtg atgtcggcgt aaatctccgg gaagctcaaa cagccttccg ggccggactc
     1381 cggctcgccc aggggattga gttccggatt gatgagaacc agcggcatga acgcgtccac
     1441 gttggcggat ttgccgtgcc gttcgaggga cgacggccgg tccgtcacgc cccgcacatc
     1501 aatgacggcc aattgcgtgg cgacgcccac ctgctgggcg gccaggccga tgcccctttt
     1561 cacatacatg gtttcgagca tgtccgcggc caactgccgg atcgccggcg taatgctctc
     1621 aatgcgctcc cctttctggc gaagcaccgg cgtgccgtat ttgacgacga ccagcggcat
     1681 gatcaggcgg gccagtggcg cagcatttcg gccaggtcgg ctatcgccgg gctttggccg
     1741 gtgcgcacgt agaagccgct ggtggtgacg ccggtcagca ggcagcgttc cgtggggaac
     1801 cccagcagct tgccgaggca gaacccggaa ttaaagtgat cgcccgcgcc cgtcgtgatc
     1861 ttcggtttgg gggtatatgg tccttgaacg atcgccgtgc cctccggccc ggaggcgagc
     1921 gcataagcgg ttggatggac gatgatcgtg tccaccggga cgcgctggtg gatatcctgg
     1981 cacagcttga gcaggccctc ctgggaatgg tcgctagggt tcaatcccag ggctttcccg
     2041 atttcaaagc cttccttttc gttcaagccc agcacgacgt cgaagtattg ctggaaagcc
     2101 gtaattaact ggagggcgct ccggatgtcc gccggggggc gtttctccgg atcggccagg
     2161 tccacgaaga gcttgcgccg ggggcctttc agcccggggc acacttcctt gagcaccgcc
     2221 gcccagatgt cgctcatgtg cgtcagcatg gtccagttaa cgaagccgac caggtcggcg
     2281 ctgccgaagc tggcgttgaa cttgtccagg ccgaaccggc ttttgatatt ctcccagttc
     2341 atctgccgga gcgattggtg cttgccgagc atgatcttgc catcctcaaa ctcaagcgcg
     2401 tcggtgtagc ccggttccgc gatggaatgc acctcggcgc gacgggcgaa atccgcgaac
     2461 accggatgca gattcgggta cccgaggatg ccgaggtagg tgactttcag cccgaaactg
     2521 gccagggcat tggccattat ggggccgttg ccgccgagtt tgttaagctg gctgacgagt
     2581 tcgatgttcg tgctccgccc cgcggcgcca gccaggcgtt ccgccagttg ggcaatggtc
     2641 gggagccgcg tatacttctc cgcgctttcc cgtttatcca ccacatggag gatgtcatca
     2701 acgaacccgt ccaggccgat gaaggcggtc attcggccaa cgcggctgcc ggcgttcagc
     2761 aattgggtag ctgtctgttc acgcataaga aagttgtcta atcaaatact gcgggcaaaa
     2821 ggccggcaac gctgcttttc cagccagccc acaaatcagg gccgcggccc ccggccccac
     2881 gggcaagggc ggattgccgc aaaacttgct tcggctgtgt gtggccacgg gatttaaata
     2941 ccgggtcgcc gggggcaagt aaagctttcg ctgtccttcc gcgggaagcc gccccgcccc
     3001 gcttcactcc ccggcaacgc ccagaatctg caggatgcgt tcaagctcgg cgtcgctgaa
     3061 gaatgaaatc tcgaggcggc ccttgccttt gttatagcgg aagtgaacct tggtcccaag
     3121 ccgttcgcgc aattggtttt cgaggttggc cacatgggcg tcccgggaca atggcagcgc
     3181 ggcggcgggc ggggtcatgg cgccggtttc gcgggccagc cagcggctga ccagtacctc
     3241 ggtttggcga acattgagcc cttccttaag aatgcggtcg gccgcccgtt gctgaagttt
     3301 ctcgtcggtc aggcccaaaa tgaccttggc gtggccaacg gacaggcgtc cctcgcggat
     3361 atgcgtttga atggcggcgg gcagccgcaa aagacgcagg gcgttggcca cggcggcgcg
     3421 gctcttgccg actttggcgg ccacttcctc ttgtttgaga tgaaactgcc cgaccagctg
     3481 cgcgtagccg cgggcctctt ccagcgggtt aaggttttcc cgctgcagat tctcgatcag
     3541 ggccagcgcc agcacggcct ggtcatccgc ctgccgcagg attaccggaa cctcgatctg
     3601 gccggccagt tgagccgccc gccagcggcg ttccccggcg atgagctcca ggtgcccgtc
     3661 ccgctcgcgg acgatcagcg gctggacgat gccctgctcc cggatggaat ccgccagttc
     3721 gcgcagcgtc tcggggggaa agtccttgcg cggctgcagc gggcaggggc gaatacgatc
     3781 cagcgggacg cgttgcacgc gcgcgcgcgg gtcggccgcc aggacggcgt ccagggcggt
     3841 atgagcggtt ggcgccggga ctggcggctt ggcgattgga ctctcgccca acagcgcccc
     3901 caggccgcgc cccaaagctg gcttagccat gccccgaaag tgtcgcagcg aagcggcgtt
     3961 gtcaattctc ctgcgcccgc cgccgggact cgtttccctt tttcaagccg gcggcggtcg
     4021 caactccggg cgcgaacggc ttttgccgcc gacccattgc ggccccgagg acgccgggcg
     4081 gtgtgaaaac tcaggccttg ggcgggcctt tcataaacgg cgcgaacagg tcaatgggaa
     4141 tcggcacgaa ggtggtggta ttatgttcgc tggagatttc gcgcatggtc tgcaggaagc
     4201 gcaactgcag ggcaatcggt tcctggctga tcatcgaggc ggcttgcacc atcttctccg
     4261 cggcctggta ttcgccttcg gcattgacga tcttggcgcg gcgctcgcgc tcggcctcgg
     4321 cctgcttggc catcgcgcgc ttcatgctgt ccggcaaggc aacatccttg acttcaaccg
     4381 cagtgacttt gacgccccac ggctcggtct ggcggtcaat gatctcctgc aacttcaggt
     4441 tgatggcctc gcgttgcgac agcaggtcgt ccaacggaga ctggcccagg acgctgcgga
     4501 gcgtggtttg cgcgatgagc gaggtggcct tgtagaaatt ctcgactttg accaccgcgt
     4561 caaccggctg cacgatgcgg aaataaacca cggcatccac cgtcaccggg acgttgtccc
     4621 gcgtcatgac ttcctgccgg gcaacatcaa tcgtcacgac gcgcaggtcc atttttacca
     4681 tgcggtcaac gaccgggatg agaaagatca ggccgggccc tttggcgccg cgggcatgca
     4741 gtttgccgag gcgaaagatg acgccccgtt cgtattcccg gaggacacgg acggcttggg
     4801 gcaggacaat cgcccccagg atgatgaggg ggacaatcca ggacgtgagg ttgaacatgc
     4861 gagcgatgag gtcttccata ggatactcca gttatcagga cgtttagcga atgggaggtt
     4921 gtttgggcac taccttcaaa gtcagaccat gaacggcgat aatctccact ggctgccccg
     4981 gctcgatggg cacttcgctg accgcgttcc agtattcgcc ctcgataaac acccggccgc
     5041 cggccgcatc aatgcgttcc cgggccgggg ccgtccggcc cagcagcgct tcgcggccca
     5101 cgcgcaccgg cagccactgc gcgcgcaggc ccgagcccac gacgaagata aagaaggccg
     5161 ccgtcaccag ggtggcgggg atgatatacg ccagcgacag ccgaaagacc gggtcggcgc
     5221 ggttgaagag catcagcgcg cccaggagga atgagacaat gccgccaaat gtcaggatgc
     5281 catgcgaagg ggcgaagata tccatgataa acaggacgat ggacaggcca atcagcgcca
     5341 gcccggccga gttgatgggg aggatcgtcg ccatatacag cacgaggatc agggcgatcg
     5401 cgcccaccac cccgggcagg atggcgccgg gattgctgag ctcgccaatg atgccataga
     5461 tcgccacgag catcaggatc atcatcaatt ccgggtgcga gagcagttgc aaaagccgtt
     5521 cctgggtggt ccggggaatc tcgacaacat tggcatccgc cgtttccagc ggcttgccgt
     5581 tgacctcgtg ctcatccagt tggcggagca aatcgggcag gtccttggca accaggtcaa
     5641 tgacgtgcag ttcgagggcc tggtcagccg tgatggcctt gctctccacc accgacgccc
     5701 gggcccattc ggcattgcgc ccgcgccggt tggcgatccc ctcgatgaag ctgcccgtga
     5761 tttgctcttg tttctccttc atcacctcgc tggtcttctc ctcgcccccg gggccaatgg
     5821 acaccgggtg agccgcgcca atgctggtgg cgggagccat ggcggcaacg tccgccgcca
     5881 gggtaatgaa gcaccccgcg ctcccggccc aggcgccctc cggggccaca tacaccacgg
     5941 tcgggaccgg cgaggcgtaa aacgcctgca caatccgctt ggtggattcg agggagccgc
     6001 caggggtatc caattggatg atgaggcaac gattctcgcg ttcggcagcg gcctcaatcg
     6061 cccgggtgat ataactggcc gtggccggcc cgatcgcgcc gttaatcttg atcaacccaa
     6121 cctccgcgcc cggcgccgcc agggcggcca ggcaggacaa taccccgaac aatattaacc
     6181 gcgaacgatt cattgcaccc ttaacctgct tcacgatacc caaattgccc gccggtgcaa
     6241 cctctattag caggggccgg ccagcgcgtc ccggtttccc accccatcaa ccttccccgc
     6301 caccagcccc cggctggccc cgaggccagg tggcgggcgg aaatctccgc gcatcctccc
     6361 ggccgtcaag cgctgccggg ttaaggccgg gcaggttgga cgagggagat gaccagcgct
     6421 tcgggccgaa gatacttccc ggcgacggcc tgcagttgct ccagcgtcac cgcctcgtag
     6481 cgggcgtctt cggtttcagt gtaggcatgg cccaggccgt agagctcatc aagggcggtg
     6541 gcggtggcga agtcgccgag gtcctggcgg gcgatcttgc gctggccaat gattttggct
     6601 ttggcacggc gaagctcctc ggcggtaagc ccctcggcgc gcaaaagctc ggcctctttg
     6661 aggagttcgg cctccacccg cgcggcctgg tcgggagccg tgccggcata gaacgcgaaa
     6721 tagcccggcg ccaggccaac gacgttgagc gcgccgacat agtaggccag ccccaactgt
     6781 tcgcggatgc gcaggaacag gcgcgagccg aggtcgctgc aggcttcatg cagcaggtcc
     6841 agcgggtagc ggtcggcatc ctgaagcgtg acgccgcggt agccgatgac cagcacggcc
     6901 tgcttcttgt cgcgggtttc ggtgacgcgc tggagcgcgt ccagcgcccg cggcccggag
     6961 tttaaggccg gcagcggcgc ggctttccag gccgcaaacg cgcgctcgac cgcggctcga
     7021 accggcgccg tttggacgtc gccataaatt gcgagcacgc aattgttggg gcgggcgaac
     7081 cgctcgtgga aggcccgaag gtccgcaacc tggaggcgcc gcaggctgcg ctcggcgccc
     7141 agcgcgtcca ggccataccc ggtctcgcca aacaaggcgc ggcgcatggc cagcagggcg
     7201 ctctggagca ggtggtcttt tcgatcacga accgcggcta actggacctc ccgctcgcgt
     7261 tccagcgccg gggccggaaa ggtggggttc aaaagcacgt ccaccagcaa atccagcccc
     7321 gccgggaaat cgctgttcag gacttcggca ttgacgccga aactgttgtt cccgccatag
     7381 ctgttcaggc tgccgcccac ggactcaatc tcgcgggcga tgtcttcagc cgaacggcgc
     7441 gccgtgccct tgagcaggag cttgccggtc aactgggtca ggccgttggt ctccggcgtc
     7501 tcggccagca ccccgccccg gaacacggcc cggaactcga caaaaggcag gcggtgatcc
     7561 tccttgacca gcaggcgcaa gccgttgggc aggtcgaact tctgcaccgg ataatcgcag
     7621 gaagtctcgc tggtgatggc cggccggggc gcggcgccgg cgggcagcag cgagtaaagc
     7681 gtgcggtttt cgggtgtgag gtattcacgc gccacgcgct ggaggtcggc gggattcaca
     7741 cgctgcacgg ccgccaggta gcgctgggaa aagttcaaat cgttggccac cagccagctt
     7801 ccccccaagt cctgcgcctg gccctgcatg gtcttgcgcg ccgacagggt ggccgagacg
     7861 aactgcttga ccgccttggc cagctcggcc gccggaaccg gggccgcctt catctgctcc
     7921 acttcggcca gcagggccgc acgcgccgcc gtgaatttat ccgcgtccac cacggcgctg
     7981 aggccgaaca aaccgggcag ccccgggctg taggtccagg cctcgaccga gtgcgccagc
     8041 ccctgcttct cgcgcacgga ttggtagagc cgcgagctgc gcccatgccc cagcaaagcg
     8101 gccagcacgt cgagcgccgg cacgtccggg tgaagcagtt cagggatatg ccaggccagg
     8161 tgaacgtggc ccagctcgat gggcgcctcc tcgcaaatct cgcgcggcgc ggcctgcttc
     8221 ggctccacgg gcaggaccac cggcggcagc gcccgggctt tggtcttcgc gtatgccttg
     8281 ccgatttggg cgaccacctc gccgggcttc acatcgccca ccacgacata aaagacgtta
     8341 ttgggcgcgt atttctcgtg gtaatacccc cggatgtcct ccggcttcag ctcgttgaaa
     8401 atgtccgggt agccgatgac cgtgaaccga tagggactgc gggtataaac cgtctcgaac
     8461 aggcggcggc tggcgcggcg ggccgggtcg tcggtgttca tgtccatctc gcgcaggatg
     8521 acctgctgtt cccgggccag ttcgtccggc ggcagcgtcg cgttctgcat cacgtcgcac
     8581 aagacatcca gcgcgaccgg cgcgcccgta ttcggcgcgt ctatgtggta aacggtgcgg
     8641 tcgaaggagg tgtaggcgtt catatagccg ccggcctcct gaacctcctg gtctatgcgg
     8701 ctgccgggcc gggtcgtggt gcccttgaag agcatgtgct ccagcacgtg cgaaagcccc
     8761 gcccccagcc accggccctc gtgaatgctg cccgccatgc accaggcctg ggccgaaaca
     8821 accggcgcgc tgtggtcctc gcgcacgatg atggtcaggc cattctccag cgtggtcagg
     8881 gtgacccccg gcggaatggc ggggaggggg tcggcggagg aggaatccgt aggcatgccg
     8941 cggcgcgtct aggatttcgc cgcgggggcc agggccgcct gtttgctttt gggcaggaac
     9001 ggtttgacga acgcctcgtt gaagtcatag ccgcccgcaa aatcggagcg gcgatccttg
     9061 ctcgttttgg ccagccaccc caccgcgacc atgttgaaaa cgatcacgcc aaaatcctcg
     9121 cagcggtgca cgccccactc ttccagcacg gttttggcca tcggcccgaa ctgttgcagc
     9181 gcgaaagtgc ggatgccctc cagcaattcc tggccggtga cgtggcggac gcgcccgcgg
     9241 gagctttggg cgatggactt ctgcgtatgc tcaagcgcct cgcgcacaaa gaggtaggct
     9301 tcccggtgat aacgcgggcc tttggcctgt atggcgtcga gcgcctgttc aaaactgact
     9361 tcttgcatgt tctaactcta ctctctcggc cacttgcgcc gacgccggcg ccgcccggca
     9421 agctttgacc gcccaaccgg ccagcagcag gctcaagact aggctcagca cgatctgttc
     9481 ctgcctggtc aagtggctca cgggggggaa tataccgcag cgggcggcgg cgtcaatcac
     9541 ccgcgcggac accgtccggc ccatccccca tcaccgtgac cgccaccgtg cgccggcggg
     9601 gccgcacatc gcattcgaga tgaaacacct gctgccaggt gcccagcacc agttcgccgt
     9661 cccggatggg aaccgtcagc gacggcccca gcagcgtggc ctgcaagtgc gaatgggcgt
     9721 tgccgtcatg ccacgcgcgt tcgtggccgt agttccggcc gggcggaatg agccggttca
     9781 gcaacgccgg caaatcctcc cgcagccccg gctccagttc gatggtgccg agggcgccgg
     9841 tgctgcccac gttgaaaaga tgaacgattc cccgccgcac ccgcgacgcg cccacaatgc
     9901 gccgcaccgc ttccgtcaga tcgtgcagat cgcccggccc tgtcgtggat accgatattt
     9961 cgtcctggta aaccatgcgc gtgctcccgt cccgggttcc ccgccctcac gccgccacga
    10021 tgttcaccag cttcttcggc agcacgatca ctttccggac cgttttgccc tccaggaacg
    10081 gctttacctt cccggaagcc cgggccgccg cctccagatc ggcctgggaa gcattggccg
    10141 gcaccgtgat gacgtcgcgc agcttgccgt taatctgcac gggaatctcg agggtatcct
    10201 cgaccagcaa ggccgggtcg aacttcggcc atggcgcgta agacagcggg ggaaccgccg
    10261 gcgcgccggc cgccccgccc gccggagagg ggccgccggc cggaagcgaa cgcgcaggcc
    10321 cggactccga aacgcccgcc gcgaaaagca ggctccaaag ctcttcggcc aggtgcggcg
    10381 cgaaggggtg cagcaggacg agaaaatcgc gcaggacggc aaccggcttc gcctcccacg
    10441 tcatcgcctc gttgataaaa accatcaggg ccgcaatggc cgtgttgaaa cgcaggccgt
    10501 ccaggtcctc ggtcaccttc ttgatgcacg catggaggac cttgagctgc gcggcggtcg
    10561 gcgggcaatc ggcgatgccg ggcgcccggc gaatgaccgc cagcagctcc cgcccgcgtt
    10621 gcggctcggc ggtcagggcc tgctcgaact cggtctcgct ctgttcgtcc acaaagagcc
    10681 gccagacgcg ccccaggaac cgatagaccc cctccacccc cttggtgctc cacggcttga
    10741 ccatctccag cggccccatg aacatctcgt agagccggaa cgcatccgcc ccatactccg
    10801 ccaggatgtc gtccgggttg attacattgc cgcgggactt ggacatcttc tggccgtcct
    10861 cgcccaaaat caacccctgg ttgacgagct tgaaaaacgg ctcgggcgtg gatacgtggc
    10921 ccaggtcata cagcacctta tgccagaacc gggcatacag caggtggaga accgcgtgct
    10981 cggtgccgcc cacgtagagg tccacgccgg gggcggacgg agccgcggcg ggcgccgcgg
    11041 cgggcgcgga ggtccccatc cagtaacgct cggcctcggg cgcgcaaaaa gccgcgcggt
    11101 tgcgggcgtc caaatagcgg aggtaatacc agcaactgcc ggcccattgc ggcatggtgt
    11161 tggtctcgcg cgtcgcgccc tcgggcaggt tcagccactc ccgcgcgcgc gccagcggcg
    11221 gctggccatc cggcgtgggt ttgtaatcct ccagcggcgg cggcatcagc ggcagcgcgg
    11281 attcgggaag cgcctcgtga taggcccgcc cgtccggccc cgtcttccaa aggatcggga
    11341 acggctcgcc ccagtagcgc tgccggctga acagccagtc ccgcagcttg tagttgacgg
    11401 tcggcttgcc ccaccccttc gcctccagcc acgcggtgat cttgcgcttg gcctccggcg
    11461 tgggcaggcc gctgatggaa atatccgcgc cggtcgagtt caccgaaatg ccgtcctcca
    11521 caaaaccgcg ccaatccacg ccggccggcg gctcgaccac ctgcacgatc ggcagcttaa
    11581 accgggaggc aaactcgaag tcgcgcgtgt cgtgccccgg caccgccatg atcgcccccg
    11641 tgccgtagct gagcagcaca tagtccgcaa tccagaccgg gatttcctgc ccgttcaccg
    11701 ggttgcgggc atagccgccc gtgaaaacgc cggtcttctc ccgggccagc tccgtgcgct
    11761 ccaaatcgct cttctgggcc accgccgccc ggtaggcctc gacctgcggc cgctgcgccg
    11821 gcgaggtcag ggcatccacc agcttatgct cgggcgccag caccatatac gtcgccccaa
    11881 acaaggtatc cgggcgggtg gtaaatacgc ggataacccc cggcgcgccg acgatgcgga
    11941 agtccacctc cgccccctcg ctgcgcccga tccagttgcg ctgcatctct ttgagcgagt
    12001 cgctccaatc gatcgtctcg aggccctcca gcaggcgctc cgcgtaggcc gtaatgcgaa
    12061 gcatccattg gcgcatcggc ttgcgcgtga cgggaaaacc gcccacctcg ctcttgccgt
    12121 ccacgacttc ctcattggcc agcaccgtgc ccaactgctc gcaccaccag accggcgcct
    12181 ccgtgacata ggccagccgc ttcgcatcgc ggtaggcgcg gcgtttcgac tccagctcgg
    12241 ccgccgggag gcccgccccg ctttgcagct ccggcgggta gggcagcgtc gcgatcggct
    12301 cggccttatt gctttgcggg ttgaaccacg aatggtaaag ctgcagaaaa atccattggg
    12361 tccagcggaa atactccggt tccgtcgtgt ccacctcccg gctccaatcg tagctgaagc
    12421 ccaggctctt gatctggcgc ttgaaggtcg caatgttctc ctcggtcgtc cggcgcgggt
    12481 gctggccggt tttgatggcg tattgctcgg cgggcaggcc gaaggcatcc cagccgatcg
    12541 gatgcaacac attgtagccc agcgcgcgcc gataccgggc caggatatcc gtggccgtat
    12601 atccctccgg atgccccacg tgcagccccg cgcccgaggg atagggaaac atgtcgagga
    12661 tataaaactt gggcaatcgc tccggccccg cgcccgcgtg ccgccaggca aacggatgcc
    12721 cggccggaac cgtctccccc gggttccatg cgcgaaacgt ctgctgctcg tcccattggc
    12781 gctgccattt gggctctatc aaatgaaacg gatactgtct gcgtccgatt gccatgagcg
    12841 cgagactatc acggaaaccg ccccggcaga caatgacgga gttgctccgc cgccccgggg
    12901 gttgactccc gcgccggttc ctgtcagggt cggcgcatgg ccggccattt catccgcccg
    12961 ctccgcccgc cccgccccgc gctccccggg cgcccgcgct cccgggccgc ttcctgtgga
    13021 taagccgccc cgccactcga cccggcctgg ccgcgcggtt gcggaccaaa ccccattctc
    13081 ccgtctccac cgcaagggcc tcatggcgct catctgcgcg ggcctggccg ccctgggcgg
    13141 ggcgggcggc ggctttgccg cggaaacatt attgcccgcc ggcgacgcgc cagcccccct
    13201 tatcagccgc catttccccg accgcctcca cgagttcgtc tggcgcaact ggcaggccgt
    13261 cgagcctgcc cggctggcaa agatcgtcgg agcctcggaa cgggacatca ccgcgctggc
    13321 cgcgtccatg ggcctgcccc cggccgccca agtgccccgc gcgctccgcg agcgagggta
    13381 tatcaccctg ctgcgccgga attggcatct cctgccgtat gagcaactgc tggagctgct
    13441 ggagctgacg cccgaacgcc tggcgttcat gctgcgcgag gaggacttcc tgtggatcaa
    13501 gctgggccag ttgaaacccc ggtgcgcgcc gctcggctac cagcctccgg acgaagccgc
    13561 ccggcgccac gccgccgaaa tccgccgcgt ggtcgaggcg gattttggcg cggaaatccg
    13621 gcgcccggcc gagccccgct tcgacttcgt ccggcagctt gcccgaaccc aaggcgcccc
    13681 gccgcggaag ccggcgcccg ccgagtctga ctccctgcgc ctggtctatt cctacgtcgc
    13741 cgtctatggc gacccgctgc tgaacccccg gttgaatcct tatcccgacg gactgctgca
    13801 acggctctcc gcggcgggca tcaatggcgt ctggctgcac gccgtcctgc gcgacctcgc
    13861 gcccggcggc ccggcgttcc ccgagtttgg cgtccaccac gaacagcgcc tggccaacct
    13921 tcagaccctg gtgaaacggg cgcgccaata cggcatcggc gtctatctct acctgaatga
    13981 accccgggcg atgcccgcgg ccttcttcgc gcaacgcccg gatctcgccg gcgtccggga
    14041 agacgatgcc accgcgctct gcacctcgca cccggcggtg cgccaatgga tggaggacgc
    14101 gctggctttc gtcttcgcgc gggtgcccgg tttggcgggc gtgtacgcga tcaccgcgtc
    14161 ggagaacctg accagttgcg cgtcgcatgg caactggcgt tcctgccagc gctgccaggc
    14221 ccgcagcgac gcggacatca tctccgaggt ggcggccgtc ctcgaggcgg gggtccaccg
    14281 cggcaacccc gcggcccgcg tgctcctgtc ggactggggc tggcgggggc atggccgcgc
    14341 cccggacatc atcgcgcgcc tgccccgatc ggcctggctg atgtcggtca gcgaatggga
    14401 cctgcccatc gagcgcggcg gcatcccgag caccgtgggc gagtattcca tctcctcggt
    14461 cgggccgggc ccgcgcgcgc tggcgcattg gcaggcggcg cggcaagccg ggctgaaaat
    14521 cggggcggaa atccagttca acaacacctg cgaaatcgcc agcctgccct gcctcccggt
    14581 gctggacctc gtggccgcgc acgcccataa gctggtttcc gcaacccaac tgaacgccca
    14641 actgatcggc tggaccatgg gcggatatcc ctcgcccaac ctccagcttg ccgcccggtt
    14701 gagccgctcc ccggcgccat ctcccgaaac cgcgctggac gagctggcgc gggaacgatt
    14761 tggccgcgcc ggcgccgcgc acgcccgcaa ggcatggtct ctgatgagcg atgcctttcg
    14821 ccagtacccc tttcatatcc gcgtggtcta taactccccc gtccaatacg gccccgccaa
    14881 cgcgctttac ccggtgaaaa ccggctaccg ggcgaccatg tggggattcc cctacgacga
    14941 cctggagggc tggcgcggtc cttacccgcc ggagattttc gcggcgcaat tcgagaagat
    15001 cgccgccggc tggcagccgg gcattgccga attgcggcag gccgtggcca aagcgcccgc
    15061 ccgccgccgc gccgaggccc gggccgacct gcggctggcc caggccgcgg ccattcattt
    15121 ccagtccgtg gccaaccagg cgcgtttcat cctcgcccgc gacgcgctcg cccgcccccc
    15181 ggcggccctc cccgccgaag accgccggcg gttgcggacg gaagccgctc gctgcctgga
    15241 gtccgaaatc gctttggcgc gccagctcta caccctcaca cggcaggatt cgcggatcgg
    15301 ctttgaaccc tcctgccaat atttctacct gcctttggac ctcgtcgaga aagtaatcgc
    15361 ctgccgctgg ctgttagaac agttcgcaca ataaaggaag cgcgccgaag ccagccccgg
    15421 cagcgccctt gcccatatcc ccctcccgat tcgggcctgc tccattggaa caggcccccg
    15481 gatatgcacc cctcccataa ccggcagaat ctccccctcc ctatctccat cggccaaact
    15541 gacctcatcc ccaaccccca gcggcttcag ccactcacag tctcctaaat cccccttttt
    15601 cccgccaacc tcccaacccg attccaactg tgccaatttg gcacagttcg acctcggcga
    15661 cgccacccgg ttggcactcc aacccaggcc tttccggatt ccttgacaaa gcaatatccg
    15721 gctccgaccc cagcccgccc atccggcatc ccctcctcca accccctccc tgccagcagc
    15781 ccaactcccc tcgatacccc catttccccg ctcccaaagc gccgtgtaca aggggtgtac
    15841 aagggatgct caccggatgc ccaaagagtt ttagcttgta cacccgatga gcatcccttg
    15901 tacacccctt gtacacggct tactactggc tatgtaacac cttggaatta tggcggatgc
    15961 gtaaggatgg cgtgggtttt ggctgaaggg gtcttgaaag gacgaaaatg gcgttatggg
    16021 gagtgtccaa aaattggacg gtctggggcg atggcggaaa gcggagcggt gggttaaggg
    16081 gcggaggcta tttcacgggc cagcggctcg ccgggggttg tgacgaggac gcgaggcgcg
    16141 tttgggatgg gggttaaggc tgccccggct ttg
//
LOCUS       DAOPQG010000082        16154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4294_length_16154_cov_14.008137, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000082 DAOPQG010000000
VERSION     DAOPQG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 16154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16154
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4294_length_16154_cov_14.008137"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            574..771
                     /gene="rpsU"
                     /locus_tag="PKI07_09870"
     CDS             574..771
                     /gene="rpsU"
                     /locus_tag="PKI07_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOC51396.1"
                     /translation="MTEIRLKKGEPVEKAIRRLKKKLDREQTLQQFRMRRRFEKPSAK
                     RRRKEKAARFAAMLKARYADE"
     gene            complement(1190..2221)
                     /gene="tatC"
                     /locus_tag="PKI07_09875"
     CDS             complement(1190..2221)
                     /gene="tatC"
                     /locus_tag="PKI07_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOC51397.1"
                     /translation="MAEDPEAERLDTPEEEEGGPVKSFLEHLEDLRWVLIKSVVALGV
                     GILLCLIAGNYVVSILKYPLTKTRAPHSGGSQVVTVFFGTNRLGAFPLSPEQQSALAL
                     GTNRSVAVRITPALLGTNQVLTWQVDDHPALTERAQSIHVDLINLSPASGFFVAFQVA
                     LYGGIVLASPFIFYFVAAFVFPALKMKERKYVYRGLTCGLGLFLVGVAFCYFFLMPLA
                     LAASQKYSQWLGFSAFQWRADDYISFVCKFMLGMGLGFEMPVVILTLVKIGVLNYAIL
                     SKARRYMIVVNLFLGAVLTTPEVITQVIMFVPLQALFEVCIWVAWYWERQDKKRAALQ
                     EAEAAVLET"
     gene            complement(2242..2523)
                     /locus_tag="PKI07_09880"
     CDS             complement(2242..2523)
                     /locus_tag="PKI07_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOC51398.1"
                     /translation="MFAGMLGGWEIVLILAVVLILFGAKRLPELAKGLGTGIREFKKA
                     TREVTDEISNAMDDTSPPPPTRLPASTTPPSPPESSPSTTPSSTSGTQA"
     gene            complement(2709..3311)
                     /locus_tag="PKI07_09885"
     CDS             complement(2709..3311)
                     /locus_tag="PKI07_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOC51399.1"
                     /translation="MKRNKFVSLLVIGLVLTMAASGCRKKPVNTTALPGSKAGKIGDA
                     SLGGLKDGDRLRTGEGVSGLPGEGIPFGDAGSHQGWLEDAQTLKTETVYFDFDSSAIR
                     SSEKSKISAVADYLKANAVKAVRVEGNCDERGTEEYNHALGERRALAVREELVRLGIT
                     PTRIDTISYGKDRPADPGHNEAAWRLNRRDEFIVLTAPGH"
     gene            complement(3335..4510)
                     /locus_tag="PKI07_09890"
     CDS             complement(3335..4510)
                     /locus_tag="PKI07_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51400.1"
                     /translation="MKRALTHSLFPVLLAAVSVLGQEVLPPITGLFEPGNTRPIWVAV
                     SGLTGEAAATLQFDLYVQGFAFTNAEAAQYLITGSNNGNLQARATDPHNKSTLVAKVY
                     SGGSLRRQVHAFADDFVQALGRKGIAQTKIAFKAENGSRSEVYIADFDGQNAQAVTKD
                     GSLVSAPCWVPGHLALYYVSYQFNHADIFYHDLSTGARRVFARYGGSNLSPAASPGGS
                     QVAMILSKDGWVDLYVGSASGGEPKRLTRSAQDESSPCWSPDGKWICFAAKERERRTL
                     CKISPAGGTMQPLGTSGMPSPTEPDWSPDGQWIAFTAQYRSGFQICVVPAGGGTAVSL
                     VEGEDPSWAPNSRTLAFCRRRGGGNYVLSLLDVPTKQVKDVSRISGIGSQSQPSWAK"
     gene            complement(4507..5331)
                     /locus_tag="PKI07_09895"
     CDS             complement(4507..5331)
                     /locus_tag="PKI07_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOC51401.1"
                     /translation="MAASSCLHGLLVLVLLVGPAFVSSKSRMSDVQPITFFPDILIDE
                     PFASPGGSPGRLPPAPTPAPPPPAPPPPPAPRPSPAQPPREEVAPPRNEGESLEVSKE
                     PARRKPEVSLVPVVRKSPAKNAAKAPASDDGQERQWQEARQRLANQLGRAASNIRSGT
                     GAATAVEEGFGSGSGPSYASYTSWVWSYFDRAWVRPQDASLENATVEVSVTIARDGTV
                     IAKRIVKRSGDAAVDASVQRTLDRVNSVERPFPEGAKDKQRTYTIPFIMKSKRGTA"
     gene            complement(5352..5774)
                     /locus_tag="PKI07_09900"
     CDS             complement(5352..5774)
                     /locus_tag="PKI07_09900"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOC51402.1"
                     /translation="MRRFSQRSSLVTLSEINITPLLDLAFVLLIIFVITTPLLEKGLE
                     LKLPKTEGQADKTVRKEDVRTVEITAQGVYALKGRPVRLDQLIRELVADFRLNPNLVV
                     YIRADENSRTRELAALISGCQQNGITRYSLRTQPPERR"
     gene            complement(5792..6523)
                     /locus_tag="PKI07_09905"
     CDS             complement(5792..6523)
                     /locus_tag="PKI07_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOC51403.1"
                     /translation="MNVPIFLAAAGVRANEMELLYVWRQATPEGKGVIYCLLVLSIIS
                     WSVMIAKVVQMRRAKKLNQYFNAEYRTQKNVLDLFDRRVQAEGCPLFTVYQAGSLELD
                     ARLKNPDGNGRKQYVSLKGMEHVKRTLENAVAQESLKLEMGLILLAIAVSGAPFLGLL
                     GTVWGVMSTFGHIAQQGVATMAAMAPGVAAALITTVAGLLVAIPSMFAYNWLVHNLRV
                     FTVELDNFAQELVSKMEREYLAEED"
     gene            complement(6909..7820)
                     /locus_tag="PKI07_09910"
     CDS             complement(6909..7820)
                     /locus_tag="PKI07_09910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOC51404.1"
                     /translation="MEPTLHRLINGDARDLAFLPDDSVHLVVTSPPYWNLKRYQENPD
                     QLGHLRDYEQFLGELAKVWRQVYRVLVPGGRLVCVVGDVCVARRSFGRHLVFPLHADI
                     CVLCRRLGFDNLNPIIWHKISNAAFEANKSSKFLGKPYEPNAIIKNDMEFILMQRKPG
                     GYRQPTHHQREASRLSKREFDEWFQQIWNIPGASTQHHPAPFPLELATRLVRMFSFTG
                     DTVLDPFTGSGTTMVAALRTRRNSIGVEIEPEYCRLAARYLKAENTDLFAPARLIFEK
                     ATVGENVCVLAEERAIYSVRPARKRLE"
     gene            complement(7825..8556)
                     /locus_tag="PKI07_09915"
     CDS             complement(7825..8556)
                     /locus_tag="PKI07_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HOC51405.1"
                     /translation="MSSSRALRELNARVQQAVAYYWRTRRSQRRRQVDAGKVDQGLRS
                     AVTGGAQMDGFIDLFSELITDAGIPSEFIFRKRAVELPGFFRPTKEWDLLVVKGRKLI
                     AAIEAKSQTGPSIGNNFNNRAEEALGSALDLWTAFRERAFLNSSQPFIGYFFMLEECE
                     ASKRPVMVQEPHFEVLPEFRGASYLRRYELFCRKLVLERHYTAAAFLTSVAEDGLRGG
                     FDTPASDLSLERFVRVLTAHLSAEI"
     gene            complement(8770..11721)
                     /locus_tag="PKI07_09920"
     CDS             complement(8770..11721)
                     /locus_tag="PKI07_09920"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOC51406.1"
                     /translation="MLLPGTALALDEAHYGVAPEPWEEGLGNHRAVVRVAQPADAVVV
                     KIPWRRRDRDPERKQIIVVDSATRQRIANVARGRLDRFEGVLAFQPPTAPGEYFIYYL
                     PFQPQPEWGNYNHDYLPPQDTAAPEWKARLPQDFGALPQAAVVRLEARKEFDSFYPME
                     VVATPAETQELLARNPAPYLLFPEDRRFPIRMTEELPLRWVKAGPGTEFHGEAQRNEF
                     YVFQIGVWAARTNLTELEVAFEGGMAGWLRCFNTGGTNWDGQPFHRQIEVPPGKVQAL
                     WIGVDVPRKARPGEHRARMIIRTRQAPPVAVSLTLKVLPEELADRGDGDLWRLARLRW
                     LDSTLAATRWPDRHDAVWRASPARDLTARTALGAIAFQKEQLLPASIRAGSQELLAAP
                     VRLTVVQNRPLSVSAAADAITHSFAQATVNGERAIRWIADAVVRCDGAAKGLSVRHEA
                     TVEADTTLKGRLVVAADGAGAAVACEDLRLELPLRPEVATYFMGLGLSARKTPASYTW
                     RWTGPYNSFWIGNAQAGLHVKLLGSSYEGPMQNLYHPKPPPSWFNGGSGGVTLTNGPQ
                     GEVRVQIFTGPLELKAGEQRVFDFALLVTPVKRLDPATHFRERYWHSTAPAPEGINVI
                     NVHHATTPNPFINYPFLAVDKLRAFCRTNQAAGRKVKIYYTVRELTSHLPELWALRSL
                     GDEVLAGGPGGGYPWLREHLGANYTTAWYTMVEGGEVDAAILTRGASRWYNFYVEGLN
                     WLVRHVPIDGLYLDDVAYDRAILKRLRKVLDRARPGGLIDLHSNTLFSMGPANQYAEF
                     FPYVDRLWFGEGFQYDAMPPEQWLVECSGIPFGLMGDMLEGGGNPWRGMLFGMTARLP
                     WPKSDPRRVWRVWDDFDIGAARMIGWWDEGCPVRTGRADVLATAYVKPGKTLIALASW
                     APDQTDVRLQWDWPALGLDAARARLVAPEIPDFQPAREWRSDEPIPVEPRRGWLIYLT
                     GSPSSPPAPPR"
     gene            12030..13592
                     /locus_tag="PKI07_09925"
     CDS             12030..13592
                     /locus_tag="PKI07_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC51407.1"
                     /translation="MPIARFFRRKSLDQLVGETVVPQHQLKRVLGPIQLTAMGVGAAV
                     GAGIFSSIGTAVAGGAEHVGAGPAIVVSFLLVAAACALAGFCYAEFAAMVPTSGSAYT
                     YAYATLGELVAWVIGWDLILEYAVGNVAVAISWSGYFQQLLRGLNLDWPGWLGVDYRT
                     ALQAARQAAELSAAQADLSALAPDVQFAAAAWAAAPQVAGLHLVFNLPAFLIVMLLTA
                     ILVLGIRESAWFNAVMVAVKLLIIFFFLLVGAFYVQPANWTPFAPHGFRGISMGAAII
                     FFAYIGFDAVSTVAEETRQPQRNMPIGILASLVVCTVLYIAVAGVLTGMAPAAQLGTA
                     EPLATAFAARGIHWAAGIVALGAVVATTSVLFVFQLGQPRILFSMARDRLLPDWAARV
                     HPRFRTPHITTILTGVLVAGFAGVSNINEVVELCNIGTLFAFVLVAAGILILRRAEPN
                     RPRPFRTPWVPWVPLGAIATCAWLMRELPEKTWKRFLYWLVAGLVIYFFYSFRRSRLH
                     NSPHRNPAGRSP"
     gene            complement(13784..14266)
                     /locus_tag="PKI07_09930"
     CDS             complement(13784..14266)
                     /locus_tag="PKI07_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51408.1"
                     /translation="MRVLILLIPLLAGCVHPRPQSGPADFHSPNLERSLQYWTANYST
                     HPVNHFYVCATDLDQGNLVEALVYWREAGRLLDYMEMSPGAEAQSWRLRPKVDREALT
                     TDEDVSLGNHVVPRRVWLRWVNQCITDGKAYVVPLKQAQAVFPKLKPPSPPANRPESP
                     "
     gene            complement(14478..14690)
                     /locus_tag="PKI07_09935"
     CDS             complement(14478..14690)
                     /locus_tag="PKI07_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC51409.1"
                     /translation="MTFQPVGADAPSLQDISLRRDAGGRVGIIGPSGSGKTTLALLLA
                     GLHPVALPGGITGGSASLFFAFATPN"
     gene            complement(15040..15261)
                     /locus_tag="PKI07_09940"
     CDS             complement(15040..15261)
                     /locus_tag="PKI07_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51410.1"
                     /translation="MNAVEFTLELSGAAVLPIPREIAMRLPKSGKARIIELTDDATDE
                     AEWRAGAYEQFLRDDAADRLRDFPPRGQK"
     gene            15403..>16154
                     /locus_tag="PKI07_09945"
     CDS             15403..>16154
                     /locus_tag="PKI07_09945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOC51411.1"
                     /translation="MARFRPSSTSASQARSNPQFALDLKMAITDRSAVQKKPNRRKRK
                     GLYSRTAPARAAAKPADILNAAPVPVHAFELREDGTILASGPPPSDGVRPKPRAEAPA
                     PVPPSPPRLVYRDEQRGLWLYHGNCLELLDAIAAKYPEGRFDTIFADPPYFLSNGGIT
                     CHAGRMVRVDKGGWDKSQGPELNHEFNREWLRRCQRVLKPNGSIWVTGTLHAIFSVGY
                     AMQQLGFKILNDIAWEKPNPPPNLSCRYFTHST"
BASE COUNT         3092 a         5199 c         4981 g         2882 t
ORIGIN      
        1 gggcctgagc cggaatggag gaatggacga gtcgcggatg atgtaaaagg acgttccaag
       61 gcgggaggga gtggacgcga acgttcggca aagccggaac gggcggcggg gtgttgtcgt
      121 gcgccgtggt tcttcggtgg gatggggcca agactggcgg gggcatttcc cctcccgggc
      181 gggggttgcc gaaaggtgac tgcttgcgtt gcccccatcg caggcggcgt attcgcccgg
      241 ttgggatgag gctggttgaa gatttattcc caggctatgc gcgccgagtg aatgattgac
      301 gggatgggtt caggaacctg ttcaagggcg ttggttgtgt ggatcaggaa ccggggcagt
      361 tggggcggcg ggttgggaaa tatcgtttct ccctcatctc ccgaaaccca ggcgctgcta
      421 acaaggggct ctgcctggcc tggcgcaaga acgcagattg agctaggcat ccgcctgggg
      481 atttgctagc ttggggttgc ttaaatctcc gggccataac agacagcagg atttggcgcc
      541 cggcagaggc aggccgtgga atgattgtta attatgaccg aaattagact gaagaaaggc
      601 gaaccggtag aaaaagccat tcgccggctt aagaagaaac tggaccgcga gcagacgttg
      661 cagcagttcc ggatgcgtcg gcgttttgaa aagcccagcg ccaagaggcg ccgcaaggaa
      721 aaggcggccc ggttcgcggc gatgctgaag gccagatatg ctgatgagtg agccgggggg
      781 ctccgctttc ggaggaggcc gccgccgctc gcggccgccg ggcggcaaaa acgcgcgcaa
      841 cagcaagggg cgcaaaggcc ggggcagccg caaacgcaaa cacgagccct tgctgccgca
      901 accggtgatt caggcctcgg gcgccgtgcg gtgcaagctt tgcggtcatc cggttgatcc
      961 caagcggctg cattttcaca tggtcaggtt tcacggcgcc gcacttcgtc ctccgccccc
     1021 ttaagcctcc caccgctccg gcgggagcaa tcggcatcca atcctgcctg gcgcataacc
     1081 cgccgcccaa ccggcgcggg cctggctgat ccgccggttc gaacaggcgc cccgtttccg
     1141 gattgccggt tcgccggggc cgcggggccc gcggacggcg gcaatctcct caggtctcca
     1201 acacggcggc ttccgcctcc tgaagcgccg cgcgtttctt atcctgccgt tcccagtacc
     1261 aggccaccca gatgcaaacc tcgaacagcg cctgcaaggg cacaaacatg atgacctggg
     1321 tgatcacttc gggcgtggtc aggaccgcgc ccagaaagag attgacgacg atcatatagc
     1381 ggcgcgcctt ggacaggatc gcataattga gaaccccgat cttgaccagc gtgagaatga
     1441 ccaccggcat ctcgaacccc agtcccatgc ccagcatgaa cttgcagaca aagctgatat
     1501 aatcgtcggc ccgccattgg aacgcggaga agccgagcca ttgcgagtat ttctgcgagg
     1561 cggccagcgc caggggcatg agaaagaaat aacaaaacgc cacgcccacg agaaacaggc
     1621 ccaggccgca ggtcagcccg cgatagacgt atttccgctc cttcatcttg agggccggaa
     1681 agacaaacgc ggcgacaaag taaaagatga acggcgaggc cagcactatg ccgccgtaga
     1741 gggctacctg gaaggccaca aagaacccgc tcgccgggct gagattgatc aggtcaacgt
     1801 ggatgctctg cgcccgctcg gtcagcgcgg gatggtcgtc aacctgccac gtgagcacct
     1861 ggttggtgcc cagaagggcc ggggtaatcc ggaccgcgac ggagcggttg gtgcccaggg
     1921 ccagcgcgct ctgctgctcc gggctgagcg ggaacgcccc caaccgattc gtgccaaaga
     1981 agaccgtcac cacctggctg ccgcccgaat gaggagcgcg ggttttggtg agcggatatt
     2041 tgaggatgga aacaacgtaa ttgccggcaa tcaggcaaag cagaatgccc acgcccaggg
     2101 cgacgacgct tttgatgagc acccaccgca ggtcttcgag gtgctccagg aaagatttta
     2161 ccgggccgcc ttcctcttcc tcgggggtat caaggcgttc cgcctcgggg tcttccgcca
     2221 tagctggaac cgttcagcga ctcaggcctg ggtgccagac gtggaagagg gggtggtgga
     2281 cggtgaggac tcgggcggcg acggcggcgt ggtgctggcg ggcaggcggg tggggggcgg
     2341 cggcgaggta tcgtccattg cgttggaaat ctcgtccgtg acctcgcggg tcgctttctt
     2401 gaattcccgg atgcccgtgc ccagcccctt ggccagttcc ggcagccttt tggccccgaa
     2461 taatatgagc acaacggcca ggatcaggac aatttcccag ccgccgagca ttcctgcaaa
     2521 catcacattc atatcttagc aaacagtgct taagtagcct aaccttgccc cgaacaatag
     2581 taaagcggca aacgcccccc acccgcgccc cccccacagc cgccgctggc agcggcgcag
     2641 gcccgcccgc gcccgccgcg cgggttcagc gcactccggt tcgccggcac cgcggtcccg
     2701 ttgcccgctt agtggcccgg cgcggtcagg acaataaact catcccgccg gttcaaccgc
     2761 caggccgctt cgttgtgccc cggatccgcc ggccggtctt tgccgtagct gatggtatca
     2821 atacgcgtcg gcgttatgcc caggcggaca agctcctcgc gcactgccag ggcgcggcgc
     2881 tcgcccaggg cgtgattgta ttcttccgtg ccgcgctcgt cgcagttgcc ctccacgcgc
     2941 acggccttga ccgcgttggc cttcagatag tccgccaccg cgctgatctt ggatttctcg
     3001 ctgctccgga tggccgagct gtcaaagtcg aaatagaccg tctcggtctt gagggtttgc
     3061 gcatcttcca gccagccttg atggctgccc gcgtcaccaa aaggaatgcc ctcgccgggc
     3121 aggccagaga ctccttcccc ggtcctgaga cggtcgccat ctttcaggcc gcccagggaa
     3181 gcatcgccga tcttgccggc cttggatccc ggcagcgcgg tggtgttgac gggcttcttg
     3241 cggcagccgg aggcggccat cgtgagcacc aagccgatta ccaataaact tacaaacttg
     3301 ttccgtttca tcttttcctg tcttttatgc gggcttattt agcccaactg ggttgtgatt
     3361 gcgagcctat tcccgaaatt cgggaaacat ctttaacttg ttttgtcggc acgtcaagca
     3421 gggagagtac gtaattccct cccccccgcc gacggcaaaa ggccagcgtc cgggagttcg
     3481 gcgcccacga agggtcttcg ccctccacca acgacacggc ggtgccgccc cccgccggca
     3541 cgacacagat ctggaagccg gaccggtatt gcgccgtgaa cgcaatccac tggccgtccg
     3601 gcgaccaatc cggctccgtc gggctgggca tcccgctggt gcccagcggc tgcatcgttc
     3661 ctcccgcggg ggagattttg cacagcgttc ggcgctctct ttccttggcc gcgaaacaga
     3721 tccatttgcc atccggcgac cagcacggcg acgactcatc ctgcgccgaa cgggtcaacc
     3781 gtttgggctc gccgccgctg gcgctgccca cgtataaatc cacccagccg tccttgctca
     3841 agatcatcgc cacctggctg ccgccgggcg aggccgccgg gctcaagttc gacccgccat
     3901 accgggcaaa cacccggcgc gcaccggtgg acagatcgtg atagaagatg tccgcgtgat
     3961 tgaactggta ggagacgtag tacagcgcca gatgccccgg cacccaacag ggggccgaca
     4021 ccagcgagcc gtccttcgtc accgcttggg cgttctgccc gtcgaagtcg gcaatataga
     4081 cctcgctgcg cgaaccgttt tcggccttga aggcgatctt ggtttgcgcg atgcctttgc
     4141 gccccagcgc ctgcacgaaa tcatccgcaa acgcatgcac ctgccgccgc aacgagccgc
     4201 cggaataaac cttggcaacg agcgtgcttt tgttgtgggg gtccgtcgcc cgggcctgca
     4261 aattgccgtt gttgctgccc gtaatcaagt attgcgccgc ctccgcgttg gtgaaggcaa
     4321 agccctggac atacagatcg aactgcaacg tcgcggccgc ttcgccggtg agcccgctca
     4381 ccgccaccca gatgggcctg gtattgccgg gctcaaataa gccggtgatc ggcggcagca
     4441 cctcctgccc caggacgctg acggcggcca acaggacggg aaaaagagaa tgagtaagag
     4501 cgcgtttcat gccgtgcctc gctttgattt cataatgaat gggatcgtat aggtgcgttg
     4561 tttatctttg gcgccctcgg gaaaggggcg ttcaacactg ttgacccggt ccagcgtccg
     4621 ctgcaccgag gcgtccacgg cggcatcgcc ggagcgcttg acaatgcgtt tggcaatgac
     4681 cgttccgtcg cgggcgatcg tcacactgac ttcgacggtg gcattttcca gcgaggcatc
     4741 ctgcggccgc acccaggcgc gatcaaagta actccagacc caggaagtat aactggcata
     4801 ggaggggccg ctgccggagc caaaaccttc ttccacggcg gtggcggcgc ccgtgccgga
     4861 gcggatattg ctcgccgcgc ggcccaattg gtttgccagc cgctgccggg cttcctgcca
     4921 ttggcgttcc tggccgtcat cactggcggg ggccttggcg gcgttcttcg ccggtgattt
     4981 cctgaccacc ggaaccaggc tgacctcggg cttcctgcgc gcgggttcct tgctcacttc
     5041 caacgactct ccctcgttcc ggggaggcgc gacttcttcc cgggggggtt gcgccgggga
     5101 cggacgcggg gccgggggcg ggggcggcgc gggcggcggc ggcgcggggg tcggcgccgg
     5161 aggaagccgg cctggagaac cgcccggact cgcaaaaggt tcgtcaatca ggatgtccgg
     5221 aaagaaggtg atcggctgca cgtcgctcat ccgggacttg gacgagacga acgccgggcc
     5281 gaccagcaag accagcacca gcaggccatg caggcagctt gacgcggcca aacacttttt
     5341 atgcagacgg ctcatcggcg ctccggcggt tgggtccgta atgaataccg cgtgattcca
     5401 ttctgctgac aaccgctaat caacgcggcc aattcccggg tccggctgtt ttcgtcggcc
     5461 cggatataaa ccaccaggtt ggggttgagc cgaaaatcgg cgaccaactc ccggatcaac
     5521 tggtccagcc tgaccggacg tcccttcaaa gcataaaccc cttgcgcggt gatttccacc
     5581 gtccgcacgt cttccttccg caccgtctta tcggcctggc cctcggtctt gggcagcttc
     5641 aactccagcc ccttctccag cagcggcgtg gtgatgacga aaatgatcag cagcacgaac
     5701 gccagatcca gcagcggcgt gatgttgatc tcgctgagcg tcaccagcga actgcgttgc
     5761 gaaaagcggc gcatggtcgg gcctcgtctg cctaatcctc ctccgccagg tactcccgct
     5821 ccatcttcga caccagctcc tgcgcgaaat tatccagttc aacggtaaac acgcgcaaat
     5881 tatgcaccag ccagttgtag gcaaacatgg agggaatcgc caccagcaac ccggccacgg
     5941 tggtgatcaa cgccgccgcc acccccggcg ccatagccgc catcgtcgcc acgccctgct
     6001 gcgcgatatg gccaaacgtg ctcataacgc cccacaccgt gcccagcagc cccaggaaag
     6061 gcgcgccgct caccgcaatc gccagcagaa tcaaccccat ctcgagcttg agcgattcct
     6121 gcgccaccgc attctccagc gtgcgtttga cgtgttccat gcctttgagc gaaacgtatt
     6181 gcttgcggcc gttgccgtcg ggattcttca gccgcgcatc caactccagg ctgcccgcct
     6241 gatacaccgt aaagagcggg cacccctccg cctgcacccg gcggtcgaag agatcaagaa
     6301 cgttcttctg ggtgcggtat tcggcgttga aatactggtt gagctttttg gcccggcgca
     6361 tctgcacgac cttggcaatc atgaccgacc aggaaatgat agacagaacg aggaggcaat
     6421 aaataacgcc cttgccttcg ggtgtggcct gccgccacac ataaagcaat tccatctcat
     6481 tcgccctgac acccgctgcc gctaaaaaaa tgggtacatt cattccgaca cgatcagtcc
     6541 gtaactccgc gcatttggtc gtgaggaata gaatcgccca agcggaactc gttcaaaaaa
     6601 caaccatcca gccccgaaaa ccaagcccac gagccttact cttaccagga tttgtaaacg
     6661 atgcaaccac aaaatctgcc cctatattac cccccctccg cggcttgtcc agaccccagt
     6721 tccaagcggt gtggctttgg ccttagccgg aatgtttccg ttggatagtc gttaaactcc
     6781 gaacaccttc cgcccgtaaa atcagcgatt tatgcttaca aggagggatg gaatggagat
     6841 agagcctgaa tcgttccggc ttaaactcct tccgcgcggg ttttgctgca gccggccaca
     6901 ggcgtggctt actcgagccg cttacgagcc ggacgaacag aataaatggc gcgttcttcg
     6961 gcaagcacac atacgttctc gccgacggtg gctttctcaa aaatcagccg tgccggggca
     7021 aacaaatccg tgttctctgc tttcaaatac cgggcggcca ggcggcagta ttcgggctcg
     7081 atttcaacgc caatgctgtt gcggcgggtt ctgagcgcgg caaccatcgt ggtgccggag
     7141 ccggtgaacg gatcaagaac cgtatcgccc gtaaacgaaa acatccggac gaggcgcgtg
     7201 gccagttcca gcggaaacgg cgcggggtgg tgctgcgtcg aagcgccggg gatattccag
     7261 atttgctgga accactcatc aaattcccgc ttgctgaggc ggctcgcctc acgctgatgg
     7321 tgggtaggct gccgatatcc gcccggcttg cgctgcatca aaataaactc catgtcgttc
     7381 ttgatgattg cgttcggctc atagggcttg ccgagaaact tgctactctt attggcctcg
     7441 aaagccgcgt tggaaatctt atgccaaatg atgggattca aattgtcgaa gccgagacgg
     7501 cggcagagaa cacagatatc cgcatgcagc ggaaaaacca gatgtcgccc aaagctccga
     7561 cgagccacgc aaacgtcgcc aaccacgcac acgaggcggc cgccaggaac cagaacacga
     7621 taaacctgcc gccagacttt ggccaactca ccgagaaact gttcatagtc ccgaagatgc
     7681 ccgagttggt ctggattctc ctgatagcgc ttcagattcc agtagggcgg cgacgtcacc
     7741 accagatgca ccgagtcatc cggcaggaat gcaagatcgc gggcgtcgcc gttaataagt
     7801 ctatgaaggg ttggctccac aagatcagat ctcggcgcta aggtgggcgg tcaacactct
     7861 cacgaagcgc tctaatgaaa ggtcagaagc gggggtatca aaaccaccgc gcagaccatc
     7921 ctccgcgacg gaggtaagaa aggcggcagc agtatagtgc cgttcaagca ccaacttgcg
     7981 gcagaacagt tcatagcgcc gcaaatacga cgcgccccga aactccggca gcacctcaaa
     8041 atgaggttcc tgcaccatca ccggtcgttt tgacgcctca cattcttcca gcatgaagaa
     8101 gtaaccaatg aacggctggc tgctattgag gaaggctcgt tcgcgaaaag ccgtccacag
     8161 gtctagagcg ctccccagcg cctcctcagc gcggttattg aaattgttgc cgatggatgg
     8221 gccggtctga gacttggcct caatggcggc aatgagcttg cgtcccttaa caaccaataa
     8281 atcccactct tttgtagggc gaaaaaaacc cggcagctcg actgcccgtt tgcggaaaat
     8341 aaactcagat ggaataccgg catctgtaat cagctcgctg aacaggtcaa taaatccgtc
     8401 catctgtgct ccacccgtca cggcgctgcg caggccctga tcaaccttgc ctgcatcaac
     8461 ctgtcggcgg cgttggctgc ggcgggtccg ccaataatac gcaaccgcct gctgcactct
     8521 ggcattcaat tctcgtagcg cgcggctcga tgacatgtaa gcgttagtat agcgaatgct
     8581 ggctatactt ccagcccttc gcggagcagt gaatttgcgc ccgaaaatga gaactgttcc
     8641 cggccagaca agaagcggaa cgcgacagtg cgaagcactg ccgggcaaag aacaaccgag
     8701 acgaccgcca cacgccagtg gaacgggtgg aaagcagagc tgctgcccga cgggaaacgc
     8761 cggggcgaat cagcgcggag gcgcgggcgg cgacgacggc gacccggtca ggtaaatgag
     8821 ccagccacgc ctgggttcga cggggatcgg ttcgtccgaa cgccattcgc gcgcgggctg
     8881 aaaatcgggt atctccggcg cgacgaggcg ggcccgggcg gcgtccaatc cgagcgccgg
     8941 ccagtcccat tgcaggcgca catcggtctg gtccggcgcc caactggcca gcgcgatgag
     9001 cgtcttgccc ggcttgacat aggccgtcgc cagcacgtcg gcgcggccag tgcgaacggg
     9061 gcagccctca tcccaccacc cgatcatgcg cgcggccccg atgtcaaaat cgtcccacac
     9121 ccgccacacc cggcgcgggt cggacttcgg ccagggcagc cgggcggtca tgccgaagag
     9181 catgccgcgc cagggattgc cgccgccctc gagcatgtct cccatcaacc caaagggaat
     9241 gccgctgcac tcgacgagcc attgctccgg cggcatcgcg tcgtattgga agccctcgcc
     9301 gaaccaaagc cggtccacgt agggaaagaa ctccgcgtac tgattggccg ggcccatgga
     9361 gaagagcgta ttggaatgca ggtctatgag tcccccgggc cgggcgcggt cgagcacctt
     9421 gcgcaggcgc ttgagaatgg cgcggtcgta ggcgacgtcg tccaggtaga ggccgtcaat
     9481 cggcacgtgg cgcacgagcc agttgagtcc ctcgacgtag aaattatacc agcgcgacgc
     9541 gccacgcgtg agaatggcgg cgtccacctc gcccccctcc accatggtgt accaggcggt
     9601 ggtgtagttg gcgcccaaat gctcgcgcag ccacggatac ccgccgcccg gcccgccggc
     9661 gagcacttcg tcgcccaggc tgcgcaaggc ccataactcg ggcaggtgag acgtcaactc
     9721 gcggacggtg tagtaaatct tcaccttgcg gcccgccgcc tggttcgtgc ggcagaacgc
     9781 gcgcagctta tccacggcga ggaagggata gttgatgaat ggattgggcg tggtggcgtg
     9841 gtggacgttg atgacgttaa tcccctcggg agccggggcc gtgctgtgcc aatagcgctc
     9901 gcggaaatga gtggcggggt cgagccgctt tacgggtgtc acgagcaggg caaaatcgaa
     9961 aacacgctgc tcgcccgcct tcagttccaa ggggccggta aagatttgaa cccgaacctc
    10021 gccttgcggc ccgttcgtga gagtgacgcc gcctgagccg ccgttgaacc agctcggcgg
    10081 cggcttggga tgatagaggt tttgcatcgg cccctcgtag ctgctgccga gcagcttgac
    10141 gtgcaggccg gcttgggcgt tgccgatcca aaaactgttg tagggtcccg tccagcgcca
    10201 ggtatagctg gccggtgttt tgcgcgcgga caggccgagg cccatgaagt aagtggccac
    10261 ctcggggcgg agaggcaact ccaggcgcag gtcttcgcaa gccaccgcag cccccgcgcc
    10321 gtcggcggcc accaccaggc ggcctttgag ggtcgtatcg gcttcaaccg tcgcttcatg
    10381 ccgcacagac aggcccttcg cggcgccgtc gcatcgaacc accgcatccg caatccaacg
    10441 aatagcccgc tcgccattca ccgtggcctg ggcaaaactg tgagttatgg catcggctgc
    10501 ggccgaaacc gataacggcc ggttttgaac aaccgtcagc cggaccggcg cggccaaaag
    10561 ctcctgcgaa cccgcgcgga tggatgccgg cagcaactgt tctttctgga acgcgatggc
    10621 gcccagggcc gtgcgggcgg ttaagtccct tgccggactg gcccgccata cagcatcgtg
    10681 gcggtccggc cagcgggtcg cggccagcgt tgaatccagc cagcgcaacc gggccagccg
    10741 ccataaatcg ccgtccccgc ggtcagccag ttcctctggc aatactttga gggttagcga
    10801 gaccgccacc ggcggggcct gccgggtgcg gattatcatc cgcgcgcggt gttcgcccgg
    10861 ccgggccttg cgcggcacgt ccaccccgat ccaaagcgcc tgcaccttgc ctggcggaac
    10921 ctcgatctgc cggtggaagg gctggccgtc ccagttcgtg ccgcccgtgt tgaagcagcg
    10981 cagccagccg gccatcccgc cctcgaacgc cacctccagc tccgtcaggt tcgtccgggc
    11041 ggcccagacg ccaatctgaa agacgtagaa ctcattccgc tgcgcctcgc catggaattc
    11101 cgtccccggc ccggctttca cccaccgcaa cggcagttcc tccgtcatcc gaatgggaaa
    11161 ccgccgatcc tcgggaaaca gcagatacgg cgccggattg cgcgcaagca actcctgcgt
    11221 ttctgccggc gtggcgacca cttccatcgg gtagaagctg tcgaattcct tccgcgcctc
    11281 caaccggaca acggcggcct gcggaagcgc gccgaagtct tgcggcagcc gcgccttcca
    11341 ctccggcgcg gcggtgtcct gcggcgggag gtaatcgtgg ttgtagttgc cccactccgg
    11401 ctgcggctga aacggcaggt aataaatgaa atactcgccc ggcgcggttg gcggctggaa
    11461 cgccagcacg ccctcgaatc ggtcaagacg cccgcgcgcc acgttggcaa tgcgctggcg
    11521 ggttgcgctg tccaccacaa tgatttgctt gcgctcggga tcccggtcgc gccgccgcca
    11581 gggaatcttc accaccaccg cgtcagccgg ctgcgcaacg cgcaccaccg cgcgatggtt
    11641 ccccagcccc tcctcccagg gctccggcgc cacgccataa tgcgcttcgt ccagggcaag
    11701 cgcggtcccc ggaagcagca agacagcggc ggccagccag gcgaacgcaa gaagaacgcg
    11761 gcggggccgg ctcctgagtc ggcgggaaac gcgggcactc cccgcgcggc aaacggtcct
    11821 caatcccctg taaatgtccc ggaacgaggg cggatgattt tccccggcaa actggcagtg
    11881 gccgacagcc tcagttttca tgggccaagt ttagccctag agccaggcgg ttaaaagagc
    11941 cgatttgccg gtttttaggc ttgcgccgcc gccgaaggta aaatctgctt tgcggaacat
    12001 tggccggcat ccggccattt taagcgcaca tgccgattgc ccgattcttt cgccgcaagt
    12061 cgctggacca attggtcggc gaaacggttg ttccgcagca ccagctcaaa cgcgtgttgg
    12121 ggccgatcca gctcaccgcg atgggggtgg gcgcggcggt gggcgcgggg attttctcca
    12181 gcattggcac cgcggtggct ggcggggcgg agcatgtcgg cgccgggccg gcaatcgtag
    12241 tgtcatttct gctggtggcg gcggcctgcg cgctggcggg cttctgctac gccgaattcg
    12301 cggccatggt gccgacgtcg ggatcggcct atacttacgc ctatgcgacc ctgggcgaac
    12361 tggtggcctg ggtcatcggc tgggacctga tcctggaata cgcggtggga aatgtggccg
    12421 tggccatttc ctggtcgggt tatttccagc aattgctgcg cgggttgaac ctggactggc
    12481 ccggctggct gggggtggat taccgcaccg cgctgcaagc ggcgcgccag gccgcggaat
    12541 tatcggcggc gcaggcggat ttgagcgcgc tggcgccgga cgtgcagttt gccgccgcgg
    12601 catgggccgc cgcgccgcag gtggccgggt tgcacctggt cttcaacctg ccggccttcc
    12661 tgatcgtgat gctgctaacc gccatcctgg tgctgggcat tcgcgaaagc gcgtggttta
    12721 acgccgtcat ggtcgccgtc aagctgctga tcattttctt cttcctgctg gtgggggcgt
    12781 tctatgtgca gccggccaac tggacgccct ttgccccgca tgggttccgg ggcatctcca
    12841 tgggcgcggc catcatcttt ttcgcctata tcgggttcga tgcggtctcg acggtcgccg
    12901 aggaaacccg ccagccgcag cgcaacatgc ccatcggcat cctggccagc cttgtggttt
    12961 gcacggtgct ttacatcgcc gtggcggggg tgctgaccgg catggccccg gcggcgcaac
    13021 tcggcaccgc cgaacccctg gcaactgcct ttgcggcgcg cggcattcat tgggccgccg
    13081 ggattgtggc cctcggcgcc gtcgtcgcca ccacttccgt gctgtttgtc ttccaactcg
    13141 gccagccgcg cattctcttc tcgatggcgc gggatcgcct gctgcccgac tgggccgccc
    13201 gggtgcaccc gcgcttccgc accccgcaca tcaccaccat tctgacgggc gtgctggtgg
    13261 cgggctttgc gggggtaagc aacatcaatg aagtcgtcga attgtgcaac atcggcacct
    13321 tgttcgcttt tgtgctggtc gccgccggga ttctgatatt gcgccgcgcc gagcccaacc
    13381 gcccgcgccc gtttcggacc ccctgggtgc cctgggtgcc tttgggcgcc atcgccacct
    13441 gcgcctggct gatgcgtgag ttgcccgaga aaacctggaa acgcttcctc tactggctcg
    13501 tcgcgggcct ggtcatctac ttcttctaca gcttccgccg cagccggttg cataattccc
    13561 ctcatcgcaa cccggcgggc cgctcgccct agggcaccgg ccaaaacaac aacgcgagcg
    13621 gttggcccgc tcaaagagag aacctcggga atgcttttcc ggcctcgcgc gcagcttgcg
    13681 agccctggtg aaatgcaagc tttaaccaat cggggagcac caaatcagaa gagacccgaa
    13741 acacgccgcg cccgggcgga cgggtgaccc ccccgccagc cgatcaggga gactcagggc
    13801 ggttggccgg tggagacggc ggcttgagtt tgggaaagac cgcctgggcc tgcttcaacg
    13861 gcacgacgta cgccttgccg tcagtgatac actggttgac ccagcggagc cagacgcggc
    13921 ggggcacgac atgattgccg aggctgacat cctcatcggt cgtgagcgcc tcccggtcca
    13981 ccttgggccg cagccgccag gactgggctt ccgcccccgg cgacatttcc atataatcca
    14041 gcagccggcc cgcttcccgc caatacacaa gggcctcgac caggttgccc tggtccaggt
    14101 ccgtggcgca gacgtagaaa tggttgaccg gatgcgtcga gtaatttgcc gtccagtatt
    14161 gcagactgcg ctcgaggttg ggcgagtgaa aatccgccgg gccggactgc ggccgcggat
    14221 gcacgcatcc ggccaacaat ggaatcagca agatcaggac acgcataagc tcagggaaga
    14281 ctcggccatg gctcgggcgg ctggctttcc gccgcccgcc tggcaacgcc catcgcaccc
    14341 gctatcactc cagctctcac gccggcgaac ttagccgcct ccctgcggcg gtcaaggctg
    14401 aactgggggc gcggcccaaa ccgaaatgcg cccgcacccg ggcagaacgg gctttctgcg
    14461 gaggcggttc tctggcatca gttgggggtg gcgaaagcaa agaagagcga ggcgctgccg
    14521 ccggtgatgc cgcccggcag ggcgacgggg tgaaggccgg caagaaggag ggcgagcgtg
    14581 gtcttgccgg agccggaagg gccaatgatt cccacacgcc ctcccgcgtc gcggcggagg
    14641 gaaatgtcct gaagcgacgg ggcatcagcc ccgacgggct ggaaggtcaa ctgccggcag
    14701 caaatcaggc ccggacgctc agcaggcgcg tgaggtgcaa atggcggatg cgagaccata
    14761 ggcgggatgg gcggccttgc aaccgagccc aaacggttcc gaacgcgggc tcctggccgg
    14821 cgtcgcgccc aacgtgaagg gtgtccactc ggcccaactc cgcggagaca tcgtcgaagc
    14881 cgcggcacga gcgaaacgcg atctgccgca gcggtccggc cgcgcccctc ggatacatcg
    14941 gcccaatcaa gaggaaccga gcgagattgc gggcgagctg ccgcacccgc atcggccggg
    15001 tgttaaaccg ccgccagagt gcggggtcaa ccttggactt catttctgcc cgcgcggcgg
    15061 aaagtccctg agccggtccg ccgcatcatc ccgcaagaac tgttcgtaag ctccggcgcg
    15121 ccactcggcc tcgtcggtgg cgtcgtccgt cagctcgatg atgcgggcct tgcccgactt
    15181 gggcaggcgc atcgcaatct cccggggaat gggcaacacc gccgcgccgc tcaactccag
    15241 cgtgaattca accgcattca tgcaataaca gtaggttgct caggcagcga ggtcaaactc
    15301 cgcaagcacg gcggcctgct gcccgcgcaa ttcgccggag gagagcggta gattgtccgc
    15361 gggcgagttg ggattgagaa ccggggtgga cataagattt ctttggcgcg ttttcgtccc
    15421 tcttccacat ccgcctccca agctcgctca aacccccaat tcgcccttga tcttaagatg
    15481 gcgatcacgg atcgatctgc cgtgcagaaa aaaccaaatc gccgcaagcg aaagggcctc
    15541 tattcacgga cggccccggc ccgtgcggcg gcaaagcccg ccgacatcct caatgccgct
    15601 ccggttccag ttcacgcctt tgaactcagg gaagacggga ccattctcgc cagtggcccg
    15661 ccgccatctg atggggtgcg gccaaagccg cgggccgaag ctcccgcccc ggtcccgcca
    15721 tcaccgccgc gcctcgttta ccgagacgaa cagcgtggtc tgtggctcta ccacggcaac
    15781 tgcctggaac tgctggacgc catcgccgcg aagtacccgg agggccggtt tgatacgatc
    15841 ttcgccgacc ctccgtattt cctttccaac ggcggcatca cctgccatgc gggccggatg
    15901 gtgcgagttg acaagggcgg ctgggataaa tcccaaggcc cggaactcaa ccatgagttt
    15961 aatcgggaat ggctgcgccg gtgccagcgc gtcctgaagc cgaacggaag catctgggtc
    16021 accggaaccc ttcatgccat cttctcggtc ggctatgcga tgcagcaact gggattcaag
    16081 atcctcaatg acatcgcctg ggaaaaacca aaccctcccc ccaatctctc ctgccgctac
    16141 ttcacacact cgac
//
LOCUS       DAOPQG010000083        15781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4430_length_15781_cov_14.894124, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000083 DAOPQG010000000
VERSION     DAOPQG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15781
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4430_length_15781_cov_14.894124"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1015..1800)
                     /locus_tag="PKI07_09950"
     CDS             complement(1015..1800)
                     /locus_tag="PKI07_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOC51412.1"
                     /translation="MSELDTIRRKTGFIIDMDGVVYHGNHLLPGTREFLEWLRVQRKK
                     FLFLTNSSRGTPRELKQKMSRLGVSLEEDHFYTSAQATAAFLRTQQPGGSAFVIGDAG
                     LTNALYQAGFTLNDVNPDYVVVGESSSYDYDKLTHAIRLVLKGARLIGTNPDLTGPTD
                     KGLVPATGALISPIELCTGAKAYYIGKPNPLIMRHALKVLGCQREETAIIGDRMDTDI
                     IAGIESEIETVLVLSGVTAREDLGKYAYRPHHVLPEVGAIVPG"
     gene            2051..3388
                     /locus_tag="PKI07_09955"
     CDS             2051..3388
                     /locus_tag="PKI07_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51413.1"
                     /translation="MKRIVPAETQHSLNPPRARPAGPGWEFNRRRFLKAVLPAGVLLA
                     APQVVRGAVLGRDGGVAPSERIVLGAIGIGSRGNYVLGCFMEEPDVRFVAICDIKAAR
                     REAIKKRADNKYGNQDCAMYRDLRELLARADLDAVLIATGPNWHGTASILAAEAGKDV
                     YCEKPCTRNIAQSLALAETFRRTGRVFQAGTQRRSLPNFIFAVDLARRGKLGRLQTLH
                     AHPGGLKAETSGWLEPEPEPDKEQVDWDLYLGPAAWRPYNKKLLDGFNFEKGGGLTGG
                     GCLEWGSHCVDLCQWANDADRTAPVEYEPQGDQLHARYANGVKLIMRNDGWLKLGTCP
                     VRFEGDTGWVETGDDGEMVASTEALLTGKGAKISGYPANLHVRDFLDAVRSRGQTRAN
                     ADAACQSHIACHAANIALFLGRRVKYDPVKNEFIGDEAANRLRSEALREPWPA"
     gene            3430..5415
                     /locus_tag="PKI07_09960"
     CDS             3430..5415
                     /locus_tag="PKI07_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC51414.1"
                     /translation="MTCSSLFSSFRTARLAPLFAVGCLVLAAGRAPAAPLPPEAAAQE
                     RALIRQLQSDAPPAEKARACKQLVLCGTAAAVPALAPLLADAHLASWARIPLEVIPGP
                     EAEAALRRGLDTLQGQLLIGVINSIAVRRDARAVGGLVKKLKDADPAVAAAAAEALGR
                     IGNGKATRALRSFLAVAPDAVRPAVAQGCILGAERRLAGGKAADAVKLYDAVRAAPVP
                     AQRRREAIRGAILARQSAGLPLLLEQLRSPDPDLFGIGLRTARELPGRPVTEALAAEL
                     ARCRPERQSFLLLALADRGDAAAMPTLLDAASRGPQKLRLTAVGILDRLGSVGSMPTL
                     LKVAADPDAGLSSAALAALARLPGPGVDSALLELLRSATGKNREVLITLAGQRHMERA
                     LPEIVRAAAEPEAGIRRAAAQAIGILGGDAQAGDLARLLEQTRNPAERADLEAALLAI
                     SSRAGARTVPHLQPLARAQDPALRILALHALASAGGPEALAAVQAAVQDSEAEVRDEA
                     VRTLSTWPNNWPDDNAVAAPLLALARSGARPAHQVLALRGYLQHLQGNKQLKDGDKAD
                     KIKDAWPLLKRPEEKRQAIAALGALPVPGALDLLVTLAGDSAVAEDACAAIVKLAGAP
                     MPAVPESQRQQALRTVVEKTAADATREEARKLLKPAP"
     gene            complement(5399..5914)
                     /locus_tag="PKI07_09965"
     CDS             complement(5399..5914)
                     /locus_tag="PKI07_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51415.1"
                     /translation="MPATPDSGADLRRLSPAELARRGADSLREHIAAQAAVAHYKHAP
                     LAFAGLDAFLADRECVRYPTRLAFEPGAMEAHQFAQPGPDPLAPDSESRVLWLRPALR
                     GHPDLALLAVAYMIPVINYGDLATDEHCVRYGATLLGMPEADFYDAVCRMADAVGAAP
                     APPPGGPTEPA"
     gene            6088..7212
                     /locus_tag="PKI07_09970"
     CDS             6088..7212
                     /locus_tag="PKI07_09970"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOC51416.1"
                     /translation="MKTLVWVASVMALGTVAGMAQPPEPPPAAAAATNGIGPKIQFAS
                     LVHDFGRARSGESIHYTYTFTNTGDATLIIKSVQPQCGCTAAGEWTRQVEPGQAGRIP
                     IQFNTHGYNGPVFKQTTVNCNVADQPAIFLQIKGTVYRPIEFTPPMTVLNISPDEDVV
                     SALVTITNNTDEPLALTAPESNNRIFAAELKTNVPGKGYQLTVTTVPPLPMGNVQGQI
                     SLRTGWTNPMAISFPVVANVQPAVLAIPSYMTLAPGPLEKAVTNSISVRNQSTNAVRL
                     SDPAVSLPGPRAEIQEMQPGKAFTVMVAFPEGFAVPPNQPVELTFKTSHPRYPVMKVP
                     IMQMPRPAAVPPPTPPPPPPPPASGPAPLPPLPSLSPPPQ"
     gene            7209..7853
                     /locus_tag="PKI07_09975"
     CDS             7209..7853
                     /locus_tag="PKI07_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOC51417.1"
                     /translation="MKKVLLEGAGVAGLGLLLALAANHLSPRRLELTRNYFPGAPPAP
                     PTVRTNPAGPADGSIPPPGSTAARLREQGLQLAGHDQAVQFFHDPRRAVEEVIFIDAR
                     DEAHYAAGHIPGAYAFDRFHPENHLVSVVPACQAARVIVFYCGGGDCDDSEHAAIMLR
                     DSLALPREKVFVYDGGFNEWSARGLPIERGARNSGDLAPATPPAAPAGNEGAPR"
     assembly_gap    7913..8012
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8013..8697
                     /locus_tag="PKI07_09980"
     CDS             <8013..8697
                     /locus_tag="PKI07_09980"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOC51418.1"
                     /translation="AAARLARWVLGGLFIYLGLSKVLHGAEFLKYVQEQLLVRNPFFA
                     GGIAAVAPWLEVLYGVWLLAGIARPAAAVIGRWQLGIVFIIMGLHKALPHPEAFLKLV
                     RQYDLVTHPLLLNAIGAALPWFEVFCGVLLLAGVAVRGTALNLVVMLVPFTVVVIWRA
                     LGIAAATGLPFCAVKFDCGCGGGEVYICAKVIENTVLLLLSAWLLRGAGRPLCLRHTL
                     RPGQGSADA"
     gene            8946..9278
                     /locus_tag="PKI07_09985"
     CDS             8946..9278
                     /locus_tag="PKI07_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOC51419.1"
                     /translation="MWCHLSALAGFVIPFGNVLGPLLVWQIKKNEFPSVVEHGKAALN
                     FQLTVLIVLLPITVLMLLLIAFCIGFLLIPVGVIIWLAGLVFAVIAGIKANNGEAYRY
                     PWSLTLIK"
     gene            complement(9294..10046)
                     /locus_tag="PKI07_09990"
     CDS             complement(9294..10046)
                     /locus_tag="PKI07_09990"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC51420.1"
                     /translation="MKRALAFGVRFLLVAGLAAGGLACRGPSGRGGVIRLLHQDRGAF
                     YTCLRGFGVDHDPDGVFQWTPGRLRISGQHYGYLATRQEYDNYRLVAEFKWGTRTWPP
                     RADKARDSGILVHATGPDEVWPTSIEAQLIEGGTGDILVVNGAYLTVAGETKGPSTSR
                     FDRPGRNPWEDKLGFRGPHEIENPHGEWNRVEITCAGDQVSVRVNGHPTLHGVKATPR
                     KGKILLQSEGAELFFRRLDLYPLRPAAPGPAR"
     gene            10163..12943
                     /locus_tag="PKI07_09995"
     CDS             10163..12943
                     /locus_tag="PKI07_09995"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOC51421.1"
                     /translation="MKPLCCVAGLAAGLGWLPLSGKAAPGWEARFAQPPNEARILKII
                     HPWPDAPAAQDQVMRQLAAQGFGGVVCNVSFDHYLESEAKWDAFTRAVRAARQAGFTL
                     WLYDERGYPSGNAGGLTLRGHPEWEARGLLAAIAESTGGPVTLALPPGKLLLAGAFPV
                     QAGQADLAGKVDLARQARDGRLSWTAPAGCWRVLAITEDRLYEGTHAEGNLHAKMPYP
                     NLLLPEPTARFVELTYGGYARHLGPDLGRFFAATFTDEPSLMSFFLKPMPYRPLPWTS
                     NLPREFRRRRGYALETAQLPALITETGAAGARLRYDFWLTVGELVSENYFGQIQARCR
                     RHRVPSGGHLLLEESVAGHVPLYGDFFRCLRRLDAPSLDCLTSLPPEVPWASARLVSS
                     AAELEGRALVMCETSDHSQRYRPPGDSRPPRTVSESEIRGTANRLIVAGVNCLTSYYS
                     FADLTDEQLCRLNEWVGRCCTALRGGHQVADCAVLYPAESLWTRFVPSRHGTHEAPGA
                     ARIEGFYRAATESLFHHARDFTVVDSRALAQAKAVPGALLHGPLRWRVVILPGADTLP
                     MAAWENLARFVRKGGVVIALGALPANSERRFPSPRVAALAEEMFGPLRPAAARGREPQ
                     SQVNARGGAGIFLPPGLEALLPQVIARLLPPDVSLPEAKSPLRVTHRRLENHEVYFVI
                     NDSDKPWAGRVEFAARGGGERWNPATGRREETLPANAAQLRLEPYGAALFRFAAADPP
                     PRAVLDPAALPVLSLQPLPAAEPTTPHGEFVRAELIPAPAPLRADSPAWQATAVLTRS
                     QVDTFLFVQFHYPAPLDLGAADCLVLETRVPPGQTTASQLLVILHEKDGGDFIAYTDR
                     SLAAPGRARSLVPLNRFQLAGWSRDADGILDLRQISDLRIGWGGYLGAEGEQIQFSLA
                     PPQLGVVLPRRP"
     gene            13000..14475
                     /locus_tag="PKI07_10000"
     CDS             13000..14475
                     /locus_tag="PKI07_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 57"
                     /protein_id="HOC51422.1"
                     /translation="MNDLPEYVGSLPNLCGAEARVAAAVRDARAQPVFSSVNRIDFGR
                     LRSAFAIALHMHQPLIPAGGPDLSTAAIISNLKYMLDNPGVGDNHNAPVFHWCYKRMG
                     QFIPQLVDEGHSPRVMLEYSGTLLHGLRQMGLHDVFDHLQRITNDPRYRACVEWLGCP
                     WGHAVAPSTPTQDYRLHVQAWQHHFAAIFGLDALRRVRGFSPAEMALPNHPDVAYEFV
                     RTLRDCGYQWVLVQEHTVEHPETGRHPERPHLPHRLVCRNARGQEAALIAIIKTQGSD
                     TKLVAQMQPAYEARGLSPVPLAGRTVPPLVTQIADGENGGVMMNEFPPKYLEVMRESS
                     GAATPVLNVSEYLEHLFARGLQETDLPVVQPLGQKRIWDRFQPGDRPERLPRVLEELK
                     RDDPRFHMEGGSWTSNISWVRGYESLLSAMEKASAHFHQKVLQPGCPTHDDRYRNALF
                     HLLCSQTSCFRYWGQGLWTDYGRELCRRLEAILTHDFPPAA"
     gene            complement(14524..15561)
                     /locus_tag="PKI07_10005"
     CDS             complement(14524..15561)
                     /locus_tag="PKI07_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51423.1"
                     /translation="MKRLLVLAGAGMLLAGAGAARAWDYEAHRAINLLALATLPTNYP
                     AFVRAPEAVERIGFLAGEMDRWRNTPDLPLKHYSFPDHYMDVEELADYGLDPRLLPVF
                     RYDFIARLALIRQAQPAKFPAPAPGTDADHTRQLVGLLPWAIAESVGKLKSGFSYLKA
                     YEADGTAEEIANARANIVYVMGVMGHLVGDAAQPLHVTRHHHGWVGENPNHYTTSRRI
                     HSWIDGGYFSQTGGVNAAALESRLRPARPVRMDDRPARPEETFQVAMLYVLAQNQQVE
                     SLYQLEKEGQFSGEGEDGRAGRAFLEKQLLVAAQMLGDFWYSAWQEAPPDTFLKGYLA
                     KRKRAAAPGGP"
BASE COUNT         2432 a         5441 c         5237 g         2571 t
ORIGIN      
        1 caccttgagt ggaaaccatg acgaccaatt tgctgaactt gacggacaca actcgcgggc
       61 ctctggcccg ctttcagcgt cctatcaaca aagcgggttg gattggcccc tcagcacgag
      121 caaccctgac tgtttttgca ttcgggcggg attgggaggt tgtggggaat ggcaattcgc
      181 tggactacag actgtagccc cgctgctgtt agtagaacat tgaaagcggg ccggaggccc
      241 gcgcgccggg gcggaaatga catattttgg ttaatttttg acttccagcg cacagtttga
      301 cggagcgtta acgttaatgg tgctttaagc ttgggcgatt tagttggaaa atacaacggc
      361 ttggcgtcat ttcaccggaa tctggagagg tatggccgtc caggccgtag ccttctcagg
      421 ccttttggct cgcgatttcc acgatataac cgtcgaatcc acgttgcctt tcggctgtaa
      481 aatcagctat ttacgattgc gaggagggat ggaatccggg tggaacccaa attattccgg
      541 cctgggtgcg ccttcatcca gatgccttgg tttttccctg cttttacgcc atgtacaagg
      601 ggtgtacaag ggatgcccat caggtgtacc agctaaaatc ctatgttttt ccggtgagca
      661 tccgttgtac accccttgta cacggcgtag gggcagggtg aatgcgaggt atgttcgggg
      721 tatgcggaag ttggcaggat tctgacttta ttctatccct ctctatactt aaaccattga
      781 ttttattggt gaaaaaggcg ggattcggcg gctatgccgt tgaaattgct aaataaaggg
      841 tctgtcggag ctacggtttg ggcaagctcg actgcccggg ccgctgtttt ttcgaatttt
      901 ctgggggatg gagtcagcgg catggccatt gaatgccggg cggctttcgc cggtaaaact
      961 ccgggggcta cgcgggccag gggggaatgg gggcggagcc gggattgttc cggttcagcc
     1021 gggcacgatg gcgccgactt cgggcaggac gtgatgtggc cggtaggcgt atttgcccag
     1081 gtcttcccgg gcggtgactc cgctcaagac cagcacggtc tcgatttcgg attcgatgcc
     1141 ggcgatgatg tcggtgtcca tgcggtcgcc gatgatggcg gtttcctcgc gctggcagcc
     1201 gaggactttc agggcgtggc gcatgatcag ggggttgggt ttgccgatgt aataggcttt
     1261 ggccccggtg cagagttcga tgggggagat gagcgcgccg gtggcgggga ccaggccctt
     1321 gtccgttggc ccggtcaggt ccgggttggt gccgatgagc cgggcgccct tcaagaccag
     1381 ccggatggcg tgggtcagtt tgtcgtagtc gtagctgctg ctttcgccga cgacgacgta
     1441 gtccgggttg acgtcgttca gggtaaagcc ggcctggtat aaggcgttgg tcaggccggc
     1501 gtcgccgatc acaaaggcgc tgccgccggg ttgttgggtg cggaggaagg cggcggttgc
     1561 ctgggcgctg gtatagaaat ggtcttcttc gaggctgacg cccaggcggg acatcttttg
     1621 cttgagctcg cgcggggtgc cgcggctgct attggtcagg aacaggaact tttttcgctg
     1681 gacgcgcagc cattccagga attcgcgggt gcccggcaac aggtggttgc cgtggtaaac
     1741 caccccgtcc atatcaatga tgaagccggt tttccggcgg attgtatcca gttcgctcat
     1801 gcccggattt attatcgcag agccgcgccc ggattcgagc caaaaagcaa acccgcggcg
     1861 gacgcccgtt ccgctttccg gccggcgcgc ccaggccgcc cggcggcggc gggccggcgg
     1921 ggggcgcggg cccaagcgcg cgggccgggc gggatggttt ttcgtgaaaa gcagcatgga
     1981 caggggggct ggctttggga catactggcg cggttgcatg agtagtgtcc gaaagaaact
     2041 gagaatgacc atgaaacgca ttgtccccgc agagacgcaa cattcgctca atccgccccg
     2101 tgcgcggccg gccggcccgg ggtgggaatt caaccgccgc cgatttctga aggcggtctt
     2161 gccggccggc gttttgctgg cggcgccgca agtggtgcgc ggggcggtgc tggggcggga
     2221 cggcggggtg gcgcccagcg agcggatcgt gctgggcgcc atcggcatcg gcagccgggg
     2281 gaattatgtg ctgggctgtt tcatggagga gccggacgtg cggtttgtgg cgatttgcga
     2341 tatcaaggcc gcgcggcgcg aggccatcaa aaagcgggcg gataacaaat acggcaacca
     2401 ggattgcgcc atgtatcgcg atctgcgcga gttgctcgcg cgcgccgacc tggacgccgt
     2461 gctgatcgcc accgggccga actggcatgg gaccgcttcg atcctggccg ccgaggccgg
     2521 caaggatgtg tattgcgaga aaccctgcac ccggaatatc gcccagagcc tggcgctggc
     2581 ggagacgttc cgccgcacgg gccgggtttt tcaggccggc acccagcggc gcagcctgcc
     2641 gaacttcatt tttgcggtgg acctggcccg gcggggcaag ctcggccggc tccagaccct
     2701 gcatgcgcat ccgggcgggc tgaaggccga gaccagcggc tggctggagc cggagccgga
     2761 gccggacaag gagcaggtgg attgggattt gtatctcggc ccggccgcgt ggcggcccta
     2821 caacaagaaa ctgctggatg gcttcaactt cgagaagggc ggcgggctga ccggcggcgg
     2881 ctgcctggaa tggggttccc attgtgtgga tctctgccaa tgggccaacg acgccgaccg
     2941 caccgcgccg gtcgagtatg agccgcaagg cgaccagttg catgcccgct acgccaacgg
     3001 cgtgaagctg atcatgcgca acgacggctg gctcaagctg ggaacctgcc cggtccgctt
     3061 cgagggcgac acgggctggg tcgagacggg cgacgacggg gaaatggttg ccagcaccga
     3121 agcgctgctc acgggcaagg gagccaaaat ctccggctac cccgccaacc tgcacgtccg
     3181 cgattttctg gacgccgtcc ggtcgcgcgg gcagacccgc gccaacgccg acgcggcctg
     3241 ccagtcgcac atcgcgtgtc acgcggccaa catcgccctg ttcctgggcc gccgggtcaa
     3301 atacgacccc gtcaaaaacg agttcatcgg cgacgaagcg gccaaccggc tccgctcgga
     3361 agccctgcgc gagccgtggc cggcgtaagc aaccttaacc cggaaagacc attcgcccag
     3421 ttcagaccta tgacctgttc aagcctgttt tcttcgttcc gaacggcgcg gctcgccccg
     3481 ctgtttgccg tggggtgtct cgtcctggcc gccggccggg cgccggccgc cccgctgccg
     3541 ccggaggccg ccgcccagga gcgcgcgttg atccgccagc ttcaatccga cgcccccccc
     3601 gccgagaagg cgcgggcgtg caagcaatta gtcctctgcg gcaccgcggc cgccgtgccg
     3661 gccctggcgc cgctgctggc ggacgcgcac ctggcctcgt gggcgcgcat ccccctggag
     3721 gtcatccccg gcccggaggc cgaggccgcc ctgcggcgcg gcctggacac gctgcaaggc
     3781 cagttgctca tcggcgtgat taattccatc gccgtgcgcc gcgacgcccg ggcggtcggc
     3841 ggcctggtga agaagctcaa ggatgcggat ccggccgtgg cggcggcggc ggcggaggcg
     3901 ctgggccgca tcgggaacgg gaaagccacg cgggcgctgc ggtcgtttct ggcggtcgcg
     3961 ccggacgcgg ttcgcccggc ggtggcccag gggtgcatcc tcggggcgga gcggcggctg
     4021 gccgggggca aggccgcgga cgccgtgaag ctctacgatg ccgtgcgcgc cgcccccgtg
     4081 cccgcgcagc ggcggcggga agccatccgg ggggccattc tggcgcggca atccgccggc
     4141 ctgccgttgc tgctcgaaca actgcgctcg ccggatccgg accttttcgg catcggcctg
     4201 cgcaccgccc gcgagctgcc cggacgcccg gtgaccgagg cgctggccgc cgaactggcg
     4261 cgctgccgcc cggagcgcca gtcgtttctg ttgctggcgc tggccgatcg cggtgacgct
     4321 gccgccatgc ccaccctgct ggacgcggcc agccgcggcc cgcaaaagct gcgcctgacc
     4381 gcggtgggca tcctggatcg cctgggcagt gtcggcagca tgcccacgct gttgaaggtg
     4441 gccgccgatc ccgacgccgg gctgtcgtcc gccgccctgg ccgccctggc ccggctgccc
     4501 ggcccgggag tggactcggc gctgctggag cttttacgga gcgccaccgg caaaaaccgc
     4561 gaggtgctca tcaccctggc cgggcagcgg cacatggagc gcgccctgcc cgagattgtg
     4621 cgcgccgccg ccgagccgga ggcggggata cgccgcgccg ccgcgcaggc gataggcatc
     4681 ctgggcggcg acgcccaggc gggggatctg gcgcggttgc tggagcagac ccggaacccg
     4741 gcggagcgcg ctgacctcga ggcggcgctg ctggccatca gcagccgcgc gggggcgcgc
     4801 accgtgccgc atctccagcc cctcgcgcgc gcccaggatc ccgccctgcg cattctcgcg
     4861 ctgcacgccc tggcctccgc cggcgggccg gaggcgctcg cggccgtcca ggccgccgtt
     4921 caggattcgg aggcggaagt gcgggacgaa gccgtccgca cgctttccac ctggcccaac
     4981 aactggcccg atgacaacgc cgttgcggcg ccgctcctgg ccctggcccg gtcgggcgcc
     5041 cggcccgccc accaggtgct cgcgctgcgc ggctacctgc agcatctgca ggggaataaa
     5101 cagctcaagg acggcgataa agcggacaaa atcaaggacg cctggccgct cctcaagcgt
     5161 cccgaggaaa agcgccaggc catcgcggcc cttggcgccc tgccggttcc cggcgcgctg
     5221 gatttgctgg tgacgttggc cggggattcc gccgtggccg aggacgcctg cgccgccatc
     5281 gtcaagctgg ccggcgcgcc catgccggcg gtgccggaga gccagcgtca gcaggcgttg
     5341 cggacggtgg tggaaaagac cgccgcggac gctacccggg aggaagcccg caaattgctc
     5401 aagccggctc cgtagggccg ccgggcggcg gcgcgggcgc ggcgcccacc gcgtcggcca
     5461 tgcggcagac ggcgtcgtag aagtccgctt cgggcatccc gagcagcgtc gcgccgtagc
     5521 gaacgcagtg ctcgtcggtg gcgaggtcgc cgtagttgat gaccgggatc atgtaggcca
     5581 ccgccagcag cgccaggtcc gggtgcccgc gcagggccgg gcgcagccag agcacccggg
     5641 actcggagtc cggggcgagc gggtccgggc cgggctgggc aaactggtgc gcctccatcg
     5701 cgcccggctc gaatgccagc cgcgtcgggt agcgcacaca ctcccggtcg gcgaggaaag
     5761 cgtccagccc ggcgaaggcg agcggggcgt gtttgtaatg cgccaccgcc gcttgcgcgg
     5821 cgatatgctc gcgcaggctg tccgcgcccc gccgggcaag ctcggcgggc gaaaggcggc
     5881 gcagatcggc gccggaatcg ggggtggccg gcatgagtga caggctagcc cattctccgg
     5941 aaaccacaag cccggcccgg cggttccgcc gccggcgcgg gccgcccgcc gcgccgggcg
     6001 ccggttttgc gcggctcaaa aagggacttt cgcggcgggg caaaatctga gatagtaaac
     6061 cgtgccctga agtttatgag taccgcaatg aagacgttag tttgggtggc atcagtgatg
     6121 gccctgggaa ccgtggcggg gatggcgcaa cccccggaac cgccgcccgc ggccgccgcc
     6181 gcgaccaacg ggatcgggcc taaaatccag tttgcgtcgc tggtgcatga ttttggccgg
     6241 gcccggagcg gggaatccat tcactatacc tacaccttca ccaatacggg ggacgccacg
     6301 ttgatcatca agagcgtgca gccgcagtgt gggtgcaccg cggcggggga gtggacgcgg
     6361 caggtcgagc ccggccaggc gggccgcatc cccatccagt tcaacaccca cggctataac
     6421 gggccggtgt tcaagcaaac cacggtcaac tgcaacgtcg ccgaccagcc cgcgattttc
     6481 ctgcaaatca aaggcacggt ctatcggccg atcgagttca ccccgcccat gacggttctt
     6541 aacatttcac ccgatgaaga cgtggtttcg gcgctggtga cgattaccaa caacaccgac
     6601 gagccgctgg cgctgacggc gcccgagagc aacaaccgca tctttgccgc tgagttgaaa
     6661 accaacgtcc ccggcaaggg ctaccagttg acggtgacga ccgtgccgcc cctgccgatg
     6721 ggaaatgtgc aggggcagat ctcgttgcgc accgggtgga ccaacccgat ggccatctcc
     6781 tttccggtcg tggccaatgt gcagccggcg gtgctggcca tcccctccta catgacgctg
     6841 gccccggggc cgctggaaaa ggcggtcacg aactccatct cggtccggaa ccagagcacg
     6901 aacgcggtgc ggttgtccga cccggcggtc agcttgcccg gcccgcgggc ggaaatccag
     6961 gagatgcaac ccggcaaggc ctttaccgta atggtggcct tcccggaggg gtttgcggtc
     7021 ccgcccaacc agccggtcga actgaccttc aagacgagcc acccgcgcta cccggtgatg
     7081 aaggtgccca tcatgcaaat gccgcgcccg gcggcggtgc cgcccccgac tccgccgccg
     7141 cccccgccgc cgccggcttc cggcccggcg ccgctgccgc cgctgccgtc gttgtcgccg
     7201 ccgccgcagt gaaaaaagtc ctgctggaag gcgccggggt ggccgggctt ggcctcctgc
     7261 tggcgttggc ggccaatcat ctgtcgcccc gccgcctgga gctgacgcgc aactacttcc
     7321 ccggcgcccc ccccgccccg cccaccgtgc gaaccaaccc ggcgggcccg gctgatggaa
     7381 gcatcccccc gccgggcagc acggccgccc gcctgcggga acagggcctg caactggccg
     7441 gccacgacca ggcggtgcaa ttcttccacg acccgcgccg cgccgtggag gaggtgatct
     7501 ttattgatgc gcgcgacgag gcgcattacg cggcgggcca tattcccggc gcgtatgcct
     7561 tcgaccgctt ccacccggag aaccacctgg tcagcgtggt cccggcctgc caggcggcgc
     7621 gggtgattgt cttctattgc ggcgggggcg actgcgacga cagcgagcat gcggcgatca
     7681 tgctgcgcga cagcctggcc ctgccccggg aaaaggtgtt cgtgtatgac ggcggcttta
     7741 acgaatggag cgcccgcggg ctgcccatcg agcggggcgc gcgcaacagc ggggacctgg
     7801 ccccggcgac tccgcccgcg gcgcccgcgg ggaacgaggg ggcgccccga tgagcgccgc
     7861 ggcggcccgg ctggcgcggt gggtgttggg ggggctgttc atctaccttg ggnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcggcgg cccggctggc gcggtgggtg
     8041 ttgggggggc tgttcatcta ccttgggttg agcaaggttc tccacggagc cgagtttctg
     8101 aagtacgtgc aggagcagtt gctggtgcgc aatccgtttt tcgccggcgg gatcgccgcc
     8161 gtcgcgccct ggctggaggt gctttacggg gtgtggctgc tcgccgggat cgcccggccg
     8221 gcggccgccg tgattggccg gtggcaactg ggcatcgtgt tcatcatcat gggcctgcac
     8281 aaggcgctgc cgcacccgga agccttcctc aagctggtgc gccaatatga tttggtgacc
     8341 cacccgctgc tgctgaacgc gatcggcgcc gcgctgccgt ggttcgaggt tttctgcggg
     8401 gtgctgctgc tggcgggcgt cgccgtccgg gggacggcgt tgaacctcgt ggtgatgctg
     8461 gtgccgttta cggtcgtggt catttggcgg gcgctgggca tcgccgcggc gacgggcctg
     8521 ccgttctgcg cggtcaagtt cgattgcggc tgcggcggcg gggaggtgta tatctgcgcc
     8581 aaagtcattg agaacacggt gttgctgctg ctttccgcct ggctgctgcg cggcgccggc
     8641 cggccgcttt gcctgcggca caccttgcgc cccggccagg gctccgcgga cgcttaaagc
     8701 ggctcggggt ggggccaaaa aggtttgcgc cagtcaagcg gcggggttga caggaggcgg
     8761 aggtggggta tggtcgccct aatgaacccg tgaaagaagc gggtttagag agtccgttta
     8821 aggctggaaa acaaacagta aactagacga cctatgaccg agaatacgat ggatcccctc
     8881 aatcaacccc gccgccaccc ccgatgggtt cgccgggcga ttcaaaccag gcccgcatgt
     8941 ggaatatgtg gtgccacctc agcgcccttg cgggattcgt tattcccttc gggaatgtgc
     9001 tgggcccctt gctggtctgg cagatcaaga agaatgagtt cccgtcggtg gtggagcacg
     9061 gcaaagccgc cctgaacttt caactgaccg tgctcatcgt gctgttgccg atcactgtcc
     9121 tcatgctttt actcatagct ttttgcattg gtttcctgct aattccggtg ggggtgatta
     9181 tctggctggc cggcctggtc tttgcggtca ttgccggcat caaagccaac aacggcgagg
     9241 cgtatcggta tccgtggagc ctgaccttga ttaagtaggg ccgcggcccg gggtcaccgg
     9301 gccgggccgg gagcggcggg gcggagcggg tagaggtcca gccggcggaa gaagagttcc
     9361 gccccttcgg attgcagcag gattttgcct ttgcgcgggg tggccttgac gccgtggagc
     9421 gtggggtggc cgttgacgcg gacgctcacc tggtcgccgg cgcaggtaat ttccacgcgg
     9481 ttccattcgc cgtgggggtt ttcgatttca tgggggccgc ggaagccgag tttgtcttcc
     9541 caggggtttc gcccggggcg gtcgaagcgg ctggtggacg ggcccttggt ttcgcccgcc
     9601 acggtcagat acgcgccgtt gaccacaagg atatcccccg tgccgccctc gatgagctgc
     9661 gcttcgatgc tggtcggcca tacctcgtcc ggcccggtcg catgcaccag gatgccgctg
     9721 tcccgcgctt tgtccgcccg cggcggccag gtccgggtgc cccacttgaa ctccgccacc
     9781 agccggtagt tgtcgtattc ctgccgcgtg gccaggtagc catagtgctg gccgctgatg
     9841 cgcagccggc ccggcgtcca ttggaagact ccgtccgggt cgtgatccac gccaaagccg
     9901 cgcaagcagg tgtagaacgc gccgcggtcc tggtgcagca ggcgaatcac cccgccccgg
     9961 ccggacggtc cccggcaggc caggcctccg gccgccaacc cggccacgag caggaaccga
    10021 accccaaaag ccaatgcgcg tttcatcgcg gcagtctggg ccacggcggg cggcgcggca
    10081 agccggaagc gtttcccgcg gggggcggcg cgcgaggaaa gcttttgccg ccgcgcggcg
    10141 ggatggtatt actatcccgg tcatgaagcc tttatgctgt gtcgccggcc tggccgccgg
    10201 gctgggctgg ctgccgctct cggggaaggc cgcccccggt tgggaggcgc ggttcgcgca
    10261 gcccccgaac gaggcgcgca tcctgaaaat cattcacccg tggccggacg cgcccgcggc
    10321 gcaggaccag gtgatgcggc agcttgccgc gcaggggttt ggcggcgtgg tttgcaatgt
    10381 ttccttcgat cactacctcg aaagcgaggc caagtgggac gcgttcacgc gcgcggttcg
    10441 cgcggcccgg caggccgggt ttaccctctg gctttatgac gagcggggtt atccttccgg
    10501 caacgccggc ggcctgacgc tgcgcggcca tcccgaatgg gaggcgcgcg gcctgctggc
    10561 ggccatcgcc gaatccaccg gcggcccggt gacgctggcg ctgcccccgg gaaaactgct
    10621 gctggccggg gcgtttccgg ttcaggccgg ccaggccgac ctggccggga aggtggacct
    10681 cgcccggcag gcgcgggacg ggcggctgag ctggacggcg ccggcaggct gctggcgggt
    10741 gctggcgatc accgaggacc gcctgtacga gggcacccac gccgagggaa acctgcatgc
    10801 taaaatgccg tatcccaacc tgcttttgcc ggagcccacg gcgcgcttcg tggaattgac
    10861 ctacggcggc tatgcccggc atctcggacc ggatttgggc cgcttcttcg ccgccacctt
    10921 cacggatgaa ccgtcgttga tgagcttttt cctcaagccg atgccctatc gcccgctgcc
    10981 gtggacgtcc aacctgccgc gcgagttccg gcggcggcgc ggctatgccc tggaaaccgc
    11041 gcaactgccg gcgctgatca ccgagaccgg cgcggcgggc gcccggctcc gctacgattt
    11101 ctggctgacg gtgggagagc tggtgtcgga aaactacttc ggccagattc aagcccgctg
    11161 ccgccggcat cgcgtcccct cgggcggcca cctgctgctg gaggaaagcg tcgccgggca
    11221 cgtgccgctc tacggggatt tctttcgctg ccttcgccgg ctggacgcgc cgagcctgga
    11281 ctgcctgacg agcctgccgc cggaagtgcc ctgggccagc gcccggctcg tgagcagcgc
    11341 ggcggagctg gaaggccgcg ccctggtgat gtgtgagacc tccgaccaca gccagcgcta
    11401 ccggcccccc ggcgattccc gcccgccccg caccgtcagc gaaagcgaaa tccgcggcac
    11461 cgccaaccgc ctgatcgtcg cgggcgttaa ctgcctcacc agctactaca gcttcgcgga
    11521 tttgaccgac gagcaactgt gccgcctcaa cgagtgggtg ggccggtgct gcaccgcgct
    11581 gcgcggcggc caccaggtcg ccgattgcgc ggtgctctac cccgccgaaa gtctctggac
    11641 ccgcttcgtc ccgtcccgcc acggcaccca cgaagcgccc ggcgccgcgc ggatcgaagg
    11701 cttttaccgc gccgccaccg aaagcctgtt tcaccatgcg cgcgacttta ccgtggtgga
    11761 cagccgcgcg ctggcccagg ccaaggccgt ccccggcgcc ctgctccacg gcccgctccg
    11821 ctggcgcgtt gtcatcctgc ccggcgcgga cacgctgccg atggccgcgt gggaaaacct
    11881 ggcccgcttt gtccgcaagg gcggcgtcgt gattgcgctc ggcgccctgc ccgccaacag
    11941 cgagcgccgg ttcccctccc cgcgggtcgc ggcgctggcg gaggaaatgt tcggccccct
    12001 ccgcccggcg gcggcgcgcg ggcgggaacc gcagtcccag gtcaacgccc ggggcggcgc
    12061 cggcatcttc ctgcctcccg gcctggaagc cctcctgccg caggtcatcg cgaggctgct
    12121 cccgccggac gtaagcctcc cggaggcgaa atcgcccctg cgcgtcaccc atcgccgcct
    12181 ggaaaaccac gaggtctatt tcgtcatcaa cgacagcgat aaaccgtggg cggggcgcgt
    12241 ggagttcgcc gcccgcggcg gcggggagcg ctggaacccg gccaccggcc ggcgggagga
    12301 aaccctgccg gccaatgccg cccagttgcg cctcgaaccc tatggcgcgg ccctcttccg
    12361 cttcgccgcc gccgacccgc cgccccgcgc cgtgctggac ccggcggcgc tgcccgtcct
    12421 ttccctgcaa ccgctgcccg ccgccgaacc caccacgccc cacggcgagt ttgtccgcgc
    12481 cgaactcatc cccgcccccg cgccgcttcg cgccgattcc cccgcctggc aggccaccgc
    12541 cgtcctgacc cggagccagg tggacacctt tctgttcgtc cagttccact accccgcccc
    12601 gctggacctg ggcgcggcgg attgtctcgt cctcgaaacc cgcgtgcccc ccgggcaaac
    12661 cacggccagc cagctcctcg tgatcctcca cgaaaaagac ggcggcgact ttatcgccta
    12721 caccgaccgc tcgctggcgg cgccgggccg ggcgcgctcc ctggttccgc tgaaccgctt
    12781 ccaactggcg ggctggtctc gcgacgccga cggcatcctg gacttgcgcc aaatcagcga
    12841 cctgcgcatc ggctggggcg gctacctggg cgccgagggc gagcaaatcc agttcagcct
    12901 ggcgccgccg caactgggcg tggtcctccc ccgccgccca tgaccgatgg ccgcgccgcc
    12961 ctttttcccc gacgccaacc cctgcctgtg tccaaaccta tgaatgacct gcctgaatat
    13021 gtgggaagcc tgcccaacct ctgcggcgcg gaggcccgcg tggccgccgc cgtgcgtgac
    13081 gcccgcgcgc agccggtgtt ttcctccgtc aaccggattg acttcggccg cctgcgcagc
    13141 gcctttgcca tcgcgctgca catgcaccag ccgctcatcc cggcgggcgg ccccgacctg
    13201 tccaccgccg ccatcatcag caacctcaaa tacatgctgg acaacccggg cgtgggcgac
    13261 aaccacaacg cgccggtctt ccactggtgc tacaagcgca tgggccagtt catcccccag
    13321 ttggtggacg aaggccattc gccgcgcgtg atgctggaat actccggcac cctgctgcac
    13381 ggcctgcgcc aaatgggcct gcatgacgtg ttcgaccacc tccagcgcat caccaacgac
    13441 ccgcgctacc gggcttgcgt cgaatggctg ggctgcccct ggggccacgc cgtggccccc
    13501 tccaccccca cccaggacta ccgcctccac gtccaggcct ggcagcacca cttcgccgcc
    13561 atcttcggcc tcgacgccct ccgccgcgtg cgcgggttct cgccggcgga aatggccctc
    13621 cccaaccacc ccgacgtcgc ctacgagttc gtccgcacgc tccgggactg cggctaccag
    13681 tgggtgctgg tccaggagca taccgtcgag caccccgaaa ccggccgcca ccccgagcgc
    13741 ccccacctgc cccaccgcct ggtctgccgc aacgcccgcg gccaggaagc cgccctcatc
    13801 gccatcatca aaacccaggg cagcgacacc aaactcgtcg cccaaatgca gcccgcctac
    13861 gaagcccgcg ggctgtcccc cgtcccgctg gccggccgca cggtgcctcc gctggtgacg
    13921 caaatcgccg acggcgaaaa cggcggcgtg atgatgaatg agtttccccc caaatacctc
    13981 gaagtcatgc gcgaaagctc cggcgccgcc actcccgtcc tgaacgtctc cgaatacctc
    14041 gaacacctct tcgcgcgggg cctccaggaa accgacctgc ccgtcgtgca gcccctcggc
    14101 cagaagcgca tctgggaccg cttccagccg ggcgaccgcc ccgagcgcct gccgcgcgtc
    14161 ctcgaagaac tcaaacgcga cgacccccgc ttccacatgg aaggcggcag ttggaccagc
    14221 aacatctcct gggtgcgcgg ctacgaatcc ctgctcagcg ccatggaaaa agccagcgcg
    14281 catttccatc aaaaagtgct ccagcccggc tgccccactc acgacgaccg ctaccgcaac
    14341 gcgctcttcc acctgctctg ctcccaaacc agttgcttcc gctactgggg ccagggcctc
    14401 tggaccgact acggccgcga actctgccgc cgcctcgaag ccatcctcac ccacgacttc
    14461 ccccccgccg cctgaaaccg ccccccgctt cctcctccgg ccgcgcggcg cggcaacgcc
    14521 cgcctacggc cccccgggcg cggcggcccg cttgcgtttg gccaggtaac ccttgaggaa
    14581 cgtatccggc ggcgcctcct gccaggccga ataccagaaa tcgcccagca tctgcgccgc
    14641 caccagcagt tgcttctcca ggaaagcccg gcccgcccgc ccgtcctcgc cctcgccgga
    14701 aaactggccc tccttctcca actggtacag cgactcgacc tgctgattct gcgccagcac
    14761 atacagcatg gccacctgga acgtctcctc cggccgggcc ggccggtcgt ccatccgcac
    14821 cggccgggcc gggcgcagcc gggattcgag cgccgccgcg ttcacgccgc ccgtctggct
    14881 gaaatagccc ccgtctatcc aactgtgaat ccgccggctg gtcgtgtaat ggttcgggtt
    14941 ttcccccacc cacccgtgat gatgccgggt gacatgcagc ggctgggccg catcccccac
    15001 caggtgcccc atcaccccca tcacgtaaac gatattggcc cgggcattgg caatctcctc
    15061 cgccgtgccg tccgcttcat acgccttcaa atacgaaaac cccgacttca acttgcccac
    15121 cgactcggcg atcgcccacg gcagcaaccc caccaactgc cgcgtatgat ccgcatccgt
    15181 ccccggggcg ggcgcgggaa acttcgccgg ctgcgcctgg cggatgaggg cgagccgggc
    15241 aatgaaatca taccgaaaca ccggcagcaa ccgggggtcc aggccataat ccgccagctc
    15301 ctccacatcc atgtaatgat ccggaaaact gtaatgcttc agcggcaggt cgggcgtatt
    15361 gcgccagcga tccatctcgc cggccaggaa cccgatccgc tccaccgcct ccggcgcgcg
    15421 cacaaacgcc gggtaattcg tcggcagtgt cgccagcgcc agcaggttga tcgcgcgatg
    15481 ggcttcgtaa tcccaggccc gcgcggcgcc cgcccccgcg agcaacatcc ccgccccggc
    15541 cagaaccagt aatcgtttca tcggcagtaa tgggttgtcc cctcacccta gccgcccccc
    15601 gggaaaaagc caagggcttt cgccgcccgg ccccggaaaa caggcttgcc cgccgccgcc
    15661 tttccggcca acctctccgc gttgcggacc cacctcacaa ccgaacgtcc gcgccgggag
    15721 cagcgtccgg cgcgggagcg cggcgcgcgc ggaacgccgg cgcccgcccg tcccgctcac
    15781 g
//
LOCUS       DAOPQG010000084        15707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4455_length_15707_cov_15.179721, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000084 DAOPQG010000000
VERSION     DAOPQG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15707
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4455_length_15707_cov_15.179721"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..666
                     /gene="gcvH"
                     /locus_tag="PKI07_10010"
     CDS             280..666
                     /gene="gcvH"
                     /locus_tag="PKI07_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOC51424.1"
                     /translation="MAKVPTELKYAKTHEWVRVSGEVATVGITDHAQRELTDVVFVEL
                     PDVGRKIRAGDACAVVESVKTANDIYSPVSGEVVETNSAIVADPAQVNSDPYTSGWFF
                     KVKLSHPGELDGLLSAKEYKAQIGGE"
     gene            811..1719
                     /gene="lipA"
                     /locus_tag="PKI07_10015"
     CDS             811..1719
                     /gene="lipA"
                     /locus_tag="PKI07_10015"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOC51425.1"
                     /translation="MPKLTHTTTPPRRRLPAWLRTSLPTTDNFARTRSLLDELRLHTV
                     CQSAACPNHWECWSKGTATFMIGGDRCTRACGFCAVNTAKPLALEADEPARVAEAVRR
                     LQLRHLVLTAVARDDLPDGGADHFRRTIEAVRAVSPRTVIEVLTPDFNAREAALDTVL
                     SARPQIFNHNLETVRRLTPTVRFRATYERSLFVLQRVKAKAGATVYTKSGLMLGLGES
                     EEELFATLADLRRAGCDLLTLGQYLQPTLRHLPVVEFIPPEKFAEYGARARALGFRHV
                     ASAPMVRSSYHADEFTLPPAPGSVVG"
     gene            complement(1705..2328)
                     /locus_tag="PKI07_10020"
     CDS             complement(1705..2328)
                     /locus_tag="PKI07_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51426.1"
                     /translation="MSSTNYRARRATLDDLGRLAALWKSMQFPAEDLARRVTEFQVIE
                     DAQGNLLGALGLQIAERQGLLHSEAFADYALAEQLRPLLWERARAVATNHGLLRLWTQ
                     ETAPFWSRCGLLPADAPAREKLPLLWRGPSAAWLTLKLKDDVETVISADQEFALFMQA
                     EKRRTERVFRQARILKAVLTLAALALLIAVAAWAFSLYLRHSQLPHH"
     gene            complement(2359..3021)
                     /locus_tag="PKI07_10025"
     CDS             complement(2359..3021)
                     /locus_tag="PKI07_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOC51427.1"
                     /translation="MPTRPDLIARLTQAGIIAIVRAPRAAQVEPLFGALLAGGLNAIE
                     ITLTTPNALEAIREARAKIGARAVTGVGTVLQAEDCRAALAAGAEFVVTPVCRPELVA
                     IAHAAGRPIMLGAYTPTEAQTAHEAGADFVKIFPADKLGPGYIKALRGPLPHLRLVPT
                     GGVDVPNVADFLKAGCAALGVGTALVSPALLQAGDWPELTRRAAAFVAAVRAAREGAF
                     SH"
     gene            complement(3034..4329)
                     /locus_tag="PKI07_10030"
     CDS             complement(3034..4329)
                     /locus_tag="PKI07_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOC51428.1"
                     /translation="MTPPVSHLKGLDYAVVQQCMHCGLCLPTCPTYDATKVERHSPRG
                     RIALLRALADDRMELTRTFAEEMYFCLGCLACMTACPAGVNYAELFEHARAEAESSGL
                     LRSPRRNLIRAFAIRWLFMDLGRLRFVGRLLRLYQQLGLQTLVRRSGALRLLPRRLRE
                     LEALTPDIQPRFSAELIAPVTPALGPRRYRVALLTGCAQDLIFSDVNRDTAEVLARNG
                     CEVVTPPKQLCCGSLHAHNGEWALAQELARKQIEQFPPDQFDAIISNAGGCGSHLKHY
                     HKLLAADPVYRDRAALWDRKVKDIHEWLMEIGVQPPARPAPLQVVTYHESCHLAHGQK
                     ITAQPRQLLRLIPNLRLVELPESTWCCGSAGIYNIVQPEMANQLLERKLRHIQSTGAA
                     IVANGNSGCLLQLINGAKQKNLPVRVVHPVTLLAEAYRR"
     gene            complement(4326..5711)
                     /locus_tag="PKI07_10035"
     CDS             complement(4326..5711)
                     /locus_tag="PKI07_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOC51429.1"
                     /translation="MALEQHMLSELRRLVGADQVLTAKEDLIPYSFDGTAALQQLPEC
                     VVFARSTEQVVGVLKLAGQARTPVVTRGSGTGLSGGSLPSPGSLVLGLAKMDKVLELD
                     RANLTLLAEAGATTQAIADAASAAGLFYPPDPGSLKISTIGGNVAENSGGLRGLKYGV
                     TRNYVMGLEVVLPEGEVLWTGNKCVKDAAGYSLRDLFIGSEGTLGVITKVLLKLIPKP
                     AAQKTLLATFPQMDQAAQTVSDIIAAQIIPCTLEFLDRTTIQCVEDFARVGLPLDCEA
                     LLLMETDGHPGAVAEEAAQMERIARQNGATEVRVAKDDAEAARLATARRSALSALARV
                     SPTTILEDATVPRSELARMIRFVAAVAAKYQLRIGTFGHMGDGNLHPTFLTDERNAEE
                     MHRVEQAFQEIFTEAIRLGGTITGEHGIGVSKKSFLPRFAGDAQMRVMRELRRALDPR
                     GILNPGKMFDA"
     gene            5826..9155
                     /locus_tag="PKI07_10040"
     CDS             5826..9155
                     /locus_tag="PKI07_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOC51430.1"
                     /translation="MTTIKPFPSRMPALLLALAVAGPGPAGALEDWQNPQLTGRNNLA
                     PHATMVICPDARTARQIGPVSNAERVKSPFYRSLNGRWKYHYGQNHRARVPDFWKPGF
                     CDQAWPAIPVPANVELEGYGIPIYVNIPYPWPKPWRPPLVPADDPNNTVNSYRRPFTV
                     PAAWAGRPVLLTFDGVNSFCQVWVNGHFVGMGKDSRTPLEFDLTPFLVPGENVLAVEN
                     FRWCDGSYLEDQDFWRLSGIFRDVYLWSPPEVHIRDFEVQTDLDARYRDATLQVNLKI
                     TNYTRRPRALFLEATLLDPAGRPVGGFGAPKPAYAEPGRETEAALHARVSNPLKWSAE
                     TPWLYQLLLTLQDNRKRVLEVIPVKVGFRKVEIKDGNLLVNGQRILIKGVNRHESDPD
                     YGQAVTVDSMIRDIHLMKQYNFNAVRTAHYPNQPAWYDLCDRYGLYLVDEANIESHGM
                     GYGAESLARVPEWRAAHLDRTLRMVERDKNHPSVIIWSLGNEAGNGTNFMATYDWIKQ
                     RDPSRPAQYERAGFARNTDIICPMYPRPSMLRQYAAGERIESKWGDEFTLPAGSRRTR
                     PFIMCEYAHAMGNSTGNLWLYWDLIYTQPFLQGGFIWDWADQALREPCARNAARTVRR
                     PKPGEPTFWAFGGDYGPPGTPSDQNFLCNGLVTPDRRPHPGLLEVKHVYQSIHCRPVD
                     LAGGSPASTSPRQPRVIEVKNGFDFLNLKDLATVSWRLTGEGRELQKGELPAPNLPPR
                     ATASLALPVKPFKPAPGVEYFLEVSFRLKHDEPWARRGHELAWEQFQLPEMAPAVAPD
                     LARLPALRLRQAPSQTTVTGRDFAATFDPRAGTLCSLKFKGVELIQAPPRPDFWRAPT
                     DNDRGRDMAQSQGIWRTAHTGAEVRAFRAEEKADTVVVTIHQRLPRVEADWTTTYTVH
                     RTGDIEVEAAFAPGRTNLPPLPRLGLQMIMPPGFDHITWLGPGPQETYCDRQDARVGL
                     YSGSVREQFFADYVEPGESGNKVEVRWAALTNRKGLGLLAVGQPRLSVNALHHTPDDL
                     QAAAHPFELPRRDFVVLNLDWRQQGLGGDNSWGYWPHEPYLIPCQAQRYHFRLRPFDA
                     DSLPGRTVAHVAGQLARQRIP"
     gene            9161..10171
                     /locus_tag="PKI07_10045"
     CDS             9161..10171
                     /locus_tag="PKI07_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51431.1"
                     /translation="MPPWLKLDWRVFAGLALAAAAVIWWGLDALEPRVNWLRVEAPRR
                     AVAGQTLTLRVHLAPLAESSRVCADLHWGTTRNGTLGCLASGGSQPVGPEGGTFDFTL
                     RVPSMKELQFVSGVIYLSRTGQWRDHTRMAFTEFIPVRDGAGARVETQLKPLRVQPPD
                     DRAGRSPRAAAVPRWLTALLFLAGAVMARRAVAAPAASAAGADTRFWQILAVLLLLAG
                     LGELFAVEDWLGARAREIARAHDLYYLRSAFQKISVSLAGAATLVWLILGGRARRPRR
                     LLLLAGGLYLGISAANLVSLHAVDRFADLSWRGITLVQALKLACALLILIGIHTARRA
                     EN"
     gene            10199..10546
                     /locus_tag="PKI07_10050"
     CDS             10199..10546
                     /locus_tag="PKI07_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOC51432.1"
                     /translation="MRVIRTCHIEAGLPSLEEARRRVLEEIRRAKREGVRVLKVIHGY
                     GSSGKGGALCVGLRKSFRLRRKEGVIRDFIPGDDFSIFTPQVLALLDAVPELRGDPDL
                     NATNEGITILWLK"
     gene            complement(10856..14221)
                     /locus_tag="PKI07_10055"
     CDS             complement(10856..14221)
                     /locus_tag="PKI07_10055"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOC51433.1"
                     /translation="MKTKLPSPARGACGTRAFRAGLAGAFLILAVAASLPAATVKLQP
                     GINGYAGAADAWLDESHKSDNYGGCSYLRVQYNSGMSDCTLVQFTLPSLSFQSLTRAT
                     LGLYYYNDYSMQKDNALGITPYRIISGMSWYENIYNGTSGHGVNWRYRDDLQTQPWTL
                     QNGAWNDKVDDGNPPRKIKRADGTPPDAIAPPAWVTWEVQRSVAQWYGGQQNNGLVLY
                     QSSFEGPGSIVAGLFHARENGLPSYSPYLEIAYAGANLYWSGAASAIWDNVAGNWDVG
                     GFPGAYGDGDFVTFRDGASHPDITIAVGGVAPGSVLISNTATAYRFSGGSLGGAGPLV
                     KQGAGPATLGAGNSYGGLTLVKAGTLIITAPNALGATSSGTVVSNGAALAFQGGVNYS
                     SAEPVTLAGNGDGGGALYAISGNNRFSGPITLAAAATISCASGELALAGINSAGFGLT
                     CDAAGTIAVNGPINGTGSSLIKNGSGLLRLSGAGVSPYSGNTVIHRGTLALADAAAIP
                     YSPVITVADGASLEVSMVTGGFELGGLANQTLQGGGSVSGDVTVQMRGRLEPGDSLGT
                     LTFLGNLTLASGALTVFELTNAPAMSGGTNDLIRVGGDLAVDNQVVTIRVLGAGPLRD
                     GSYQLFHYAGAKTGAFNPTPVFLSGAPAPGSTVWIDESVTNQIRLEVVTPVPTVTSVT
                     TRPNPSLPEETVTLRALVNALNAETNVTPTGSVTFTTNGVPLGPPVPLDNGQALMDTA
                     GLAHGLTPILAEYPGNSQFLGSTGSVVHLANTPPVPGPHILYVLQNEKQLLPVAALLA
                     EDLDPDGDPLNLADVSALSTNGGPAALVGTSSLAWLPKPDYVGGDLLTYTLSDPYTSV
                     TGAICFHVLAGDDLTNSVLDISNLGGGVMALTATGIPGWPYVVQSVPSLTLPLSWQTL
                     GTNLANSAGWFQFTDTVATNLERYYRVFTAPNPTAWQIYDAELLTLETTGATSPFSVM
                     LRESPSLLSRGVTLIKPSATSHCTISSGFDVFTEISLDQGHSWHPATNGPVSLILTGG
                     LPLNQFPGESLPPLMGRYLSPPEQPELYSPGILIKDLALHGFSATFAPPNPGEVLLYH
                     LDAMLDFWVSFDGGQTFQPHSAPARVVIQIKCR"
     gene            14527..15408
                     /locus_tag="PKI07_10060"
     CDS             14527..15408
                     /locus_tag="PKI07_10060"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOC51434.1"
                     /translation="MSTAYYQALDAIIQHVEALQERGVRFIPVAPETLARLREPPPVA
                     VPAGPARSVPSPFPSATPALPTAPAAPPPPLAASPPLSLEARAAAMAGLRQRVLACVK
                     CPRLAAARRNVVFGVGALDARLMFVGEAPGADEDAQGEPFVGRAGELLTKIICTMGLT
                     REAVYIGNILKCRPDTPGQASGNRKPTPEEMQTCLPYLHEQIDIIRPEVIVALGATAV
                     EGLLGRTAGITRIRGQWHTYRGTPLMPTYHPAYLLRKTSLDEKRRVWEDMLLVMERLR
                     LPISEKQRNFFLKALTG"
BASE COUNT         2544 a         5178 c         5340 g         2645 t
ORIGIN      
        1 tgaatcgttc tggctaaggc cggcgcaggg attcccgcag gcggggccgt gtggtggggc
       61 ggcctggatt ttggcttgag ctcccaggct caatggctcg gcaaggccat tcccggacgg
      121 tttgggtggc ttgcatacgc atcagggaga gattcaaggc aggtcgcttt ttcgcaactt
      181 cagattacgt tgcaaggcaa gaaaccggga cgcgcccaaa agccgtgttg ccgcgaaaac
      241 cggattgacc gcttgccttg tccctgactc aataggcgca tggctaaagt tcctacggaa
      301 ttgaagtatg cgaaaacgca cgaatgggtg cgcgtgtcgg gcgaggtggc gacggtgggc
      361 atcaccgacc acgcccagcg cgagctgacc gacgtggtct tcgtcgagct gcctgacgtc
      421 ggccggaaga tcagggccgg cgacgcctgc gcggtggtcg agtcggtgaa aacggccaac
      481 gatatttact cgccggtcag cggcgaagtg gtcgaaacca actccgcgat agtcgccgac
      541 ccggcgcagg tcaatagcga cccgtacacc agcggatggt tcttcaaagt caaactatcc
      601 cacccggggg agttggacgg actgctctcc gccaaggaat acaaggcgca gatcggcggg
      661 gagtgaccgc gggaggggaa ggcgaacttc cccgcgcgcc cgggcattag ccgcggcctg
      721 gatgggccgt ggctcgcgcg gagcctcgtc cgcgccattt gctaattggc ccgcgctcgg
      781 ccattgtatg gaggtgaaac caaaaccgac gtgccgaaac tgactcacac taccaccccg
      841 ccccgccgcc gcctgcccgc ctggctgcgc acgagcctgc ccacgacgga caatttcgcc
      901 cgcacgcggt cgctgctgga cgagttgcgg ctgcatacgg tctgccagag cgccgcctgc
      961 ccgaaccatt gggaatgctg gagcaagggc accgccacct tcatgatcgg cggcgaccgc
     1021 tgcacccggg cttgcggttt ctgcgccgtg aacaccgcca aacccctcgc gctggaagcc
     1081 gacgaacccg cccgcgtcgc ggaagccgtc cggcgcctgc aactccggca cctggtcctc
     1141 accgccgtcg cccgcgacga cctgcccgac ggcggcgccg accatttccg gcgcaccatc
     1201 gaggcggtgc gcgcggtgag cccccgcacg gtgatcgagg tgctcacccc ggatttcaac
     1261 gcgcgggagg cggcgctcga caccgtgctg tccgcgcggc cgcagatttt caaccacaac
     1321 ctcgagaccg tgcggcggct tacgccaacg gtgcggttcc gggcgacgta tgagcgttcg
     1381 ctgttcgtgc tgcaacgggt caaggcgaag gccggcgcga cggtctatac caagtccggc
     1441 ctgatgctgg ggctggggga atccgaggag gaattattcg ccaccctggc cgacttgcgc
     1501 cgggccggct gcgacctgct caccctgggg caatacctgc agccgacgct ccggcatctg
     1561 ccggtggtgg agtttattcc gccggagaaa tttgcggaat acggcgcgcg cgcccgggcg
     1621 ctggggttcc ggcacgtcgc cagcgcgccg atggttcgca gctcctatca cgccgacgag
     1681 ttcaccctcc cgcccgcgcc gggttcagtg gtggggtagc tgcgagtggc gcagatacag
     1741 gctgaacgcc caggctgcca ccgcgatcag cagcgccagg gccgccaggg ttagcaccgc
     1801 cttcaggatg cgcgcctgcc gaaagacccg ctcggtgcgc cgcttttccg cttgcatgaa
     1861 cagggcgaat tcctggtcgg cggagatcac cgtttccaca tcgtccttca gcttcagggt
     1921 cagccaggcg gcggatggcc cgcgccacag gagcggcagt ttttcccgcg ccggcgcgtc
     1981 agccgggagc aggccgcagc ggctccagaa gggcgccgtt tcctgcgtcc agagccgcag
     2041 cagtccgtgg ttggtcgcga cggcgcgcgc gcgctcccac agcagcggcc gcagttgttc
     2101 ggccagcgcg tagtcggcaa aggcctcgct gtggagcagg ccctggcgct cggcgatttg
     2161 aagccccaac gcgcccaaca ggttcccctg cgcatcctcg atcacctgga attccgtcac
     2221 gcgccgggcc aggtcttcgg ccgggaattg catggacttc cagagagccg ccagccggcc
     2281 gagatcatcc agcgtcgccc gccgcgcgcg atagttcgtc gagctcaccc gctcaattta
     2341 ggcagcttcc gggccgaatc aatgcgaaaa ggcgccctcc cgcgccgcgc ggacggcggc
     2401 gacgaaagcg gcggcccggc gggtcagctc cggccagtcc cccgcctgga ggagggcggg
     2461 ggagaccagc gccgtgccga cgcccagcgc cgcgcagccg gccttcagga aatccgcgac
     2521 gttcggcacg tccacgccgc cggttggcac caggcgcaaa tgcggcaacg gcccgcgcag
     2581 cgccttgata taccccggcc ccagcttgtc cgccggaaag atcttcacga agtccgcgcc
     2641 cgcttcgtgc gcggtttgcg cttccgtggg ggtataggcg ccgagcatga tgggacgccc
     2701 ggcggcatgc gcaatggcca ccagctccgg gcggcagacg ggcgtgacca caaactccgc
     2761 ccccgccgcc agggccgccc ggcagtcctc cgcctggagc acggtgccga cgcctgtcac
     2821 ggcgcgggcg ccgattttgg cccgcgcttc gcggatggcc tccagcgcgt tgggggtggt
     2881 cagggtaatt tcgatggcgt tcaggccccc ggccaggagc gcgccaaaca gcggctccac
     2941 ctgggcggcc cgcggcgcgc ggacaatggc gatgatcccc gcctgggtca ggcgcgcgat
     3001 aaggtccgga cgggtgggca taaagctatt cggtcatcgc cggtaggctt ccgcgagcag
     3061 cgtgaccggg tggaccacgc gcaccgggag gttcttctgc ttcgcgccgt tgatgagttg
     3121 caggaggcac cccgaattgc cattggccac gatggccgcg ccggtggatt gaatatgccg
     3181 gagcttgcgt tccaggagct ggttggccat ctccggctgc acgatgttgt agatgccggc
     3241 gctgccgcag caccaggtgc tttcgggcag ctcgaccaac cgcaggttgg ggatgagccg
     3301 cagcagttgg cggggctggg cggtgatctt ttgcccatgt gccaggtggc aggattcgtg
     3361 gtaggtcacc acctgcaacg gcgcgggccg ggcgggcggc tggacgccga tctccatcag
     3421 ccactcgtgg atgtctttca ccttccggtc ccagagcgcg gcccggtcgc ggtaaaccgg
     3481 gtcggcggcc agcagtttgt ggtagtgctt gaggtgcgag ccgcagccgc cggcgttgct
     3541 gatgatggcg tcgaactggt cgggcggaaa ctgctcgatt tgcttgcgcg ccagttcctg
     3601 cgccagcgcc cattcgccgt tgtgggcgtg gagcgagccg cagcagagct gcttcggcgg
     3661 ggtgacgact tcgcagccgt tgcgcgccag cacttccgcc gtgtcccggt tgacatcgct
     3721 gaagatcagg tcctgggcgc agccggtgag cagggcgacg cggtagcgcc gggggcccag
     3781 ggcgggcgtg acgggcgcga tcagctcggc ggagaaccgg ggctgaatat cgggcgtcaa
     3841 cgcctccagc tcgcgcaggc gccggggcag gagccggagc gcgccgctgc gccggaccag
     3901 tgtttgcagg ccgagctgct ggtaaagccg cagcagccgg cccacgaacc gcaggcggcc
     3961 caggtccatg aacagccagc ggatggcaaa cgcccgaatc agattgcgcc gtggcgagcg
     4021 gagcaggccg ctggactcgg cttcggcgcg ggcatgctca aacagctcgg cgtaattgac
     4081 ccccgccggg caggcggtca tgcaggccag gcagccgagg cagaaataca tttcttccgc
     4141 aaaggtgcgc gtcaattcca tccggtcgtc ggccagggcg cgcaggagcg cgatgcgccc
     4201 gcgcgggctg tggcgctcga ccttggtcgc gtcgtaggtc gggcaggtgg gcaggcataa
     4261 cccgcagtgc atgcactgct gcaccaccgc gtagtcgagg cccttcaggt gagaaaccgg
     4321 gggtgtcacg cgtcaaacat ctttcccggg ttcagaatcc cgcgcgggtc gagcgcccgg
     4381 cgcagctcgc gcatgacgcg catctgggcg tccccggcaa accggggcag gaaggatttc
     4441 ttggacacgc caatgccatg ctcgccggtg atggtgccgc ccaggcgaat cgcctcggtg
     4501 aagatttcct ggaaagcctg ctcgacccgg tgcatttcct ccgcgttgcg ctcgtcggtg
     4561 aggaaggtgg gatgcaggtt gccgtcgccc atgtggccga aggtgccgat gcgcaactgg
     4621 tatttggcgg cgacggcggc cacgaagcga atcatccggg ccagctcgct gcggggcacg
     4681 gtggcgtctt cgaggatggt cgtcggggaa acccgggcca gggcggagag ggcgctgcgc
     4741 cgcgccgtgg ccagtcgggc ggcctcggcg tcgtctttgg caacgcgcac ctccgtcgcc
     4801 ccgttctggc gggcgatgcg ttccatttgg gcggcttcct cggcaaccgc gccggggtgg
     4861 ccgtcggttt ccataagcag cagcgcctcg caatccagcg gcaggccgac ccgggcgaag
     4921 tcttcaacgc actggatggt ggtgcggtcc aggaactcca gcgtgcaggg aataatttgg
     4981 gcggcaataa tgtccgaaac cgtctgggcg gcctggtcca tctggggaaa ggtcgccagc
     5041 agggttttct gggccgccgg tttggggatg agcttgagca gcactttggt gatgactccc
     5101 agcgtgccct cggagccgat gaacagatcg cgcagtgagt agcccgccgc atccttcacg
     5161 cacttattgc ctgtccagag cacctcgccc tccggcagca ccacctccag ccccatgacg
     5221 taattgcgcg tgacgccgta tttgaggccg cgcagcccgc cggagttctc ggctacgttg
     5281 ccgccgatgg tggatatctt cagggaaccg gggtccgggg gatagaacag gcccgccgcg
     5341 ctcgcggcgt cggcgatggc ctgcgtcgtg gcgcccgcct cggccagcag ggtgagattg
     5401 gcgcggtcca gttccagcac tttgtccatt ttggccaggc ccaggacgag gctcccggga
     5461 ctgggcaggc tgccgccgct caggccggtg ccggagccgc gcgtcacgac gggggtccgg
     5521 gcctggccgg ccagcttgag cacgccgacg acctgttcgg tggagcgggc aaagacaacg
     5581 cactccggca gttgctgcag cgcggcggtg ccgtcgaagg agtaggggat caggtcttcc
     5641 tttgccgtca ggacctggtc ggcgcccacg agacgccgca gttcggagag catgtgttgc
     5701 tcgagtgcca taacagttcg ccccgacagg atgaaggagc cgggccgcca ggtcaaggaa
     5761 acgcccaccg cgcgggcggg gtacgagttg ccacccagcg ggtctttcgg ctacatcatg
     5821 ggcgtatgac aacgatcaaa ccgttcccgt cccggatgcc ggcgctgctt ttggcgctgg
     5881 ccgtcgccgg ccccggcccg gcgggcgcgc tcgaagactg gcaaaatccc cagctgacgg
     5941 gccgcaacaa cctggcgccc cacgccacca tggtcatctg cccggacgcc aggaccgcgc
     6001 ggcagatcgg cccggtatcg aatgccgagc gggtgaaatc gcccttctat cgctcgctca
     6061 acggccggtg gaaataccat tacggccaga accaccgcgc ccgcgtgccg gatttctgga
     6121 aacccggatt ttgcgaccag gcctggccgg ccatcccggt gccggccaac gtcgagctgg
     6181 agggctatgg catcccgatt tacgtgaaca tcccctaccc gtggcccaaa ccctggcgcc
     6241 cgccgctggt gccggccgat gacccgaaca ataccgtcaa ttcctaccgc cgcccgttca
     6301 ccgtgcccgc ggcctgggcg ggccgcccgg tgctgctgac ctttgacggc gtgaactcct
     6361 tttgccaggt ctgggtcaac ggccacttcg tcggcatggg caaggattcc cgaacgccgc
     6421 tggagttcga cctcacgccg ttccttgtcc ccggcgagaa cgtgctggcg gtcgagaact
     6481 tccgctggtg cgacggctcc tatcttgaag atcaggactt ctggcgcctg agcggcattt
     6541 tccgggatgt ctatctctgg tcgccgcccg aggtccatat ccgcgacttc gaggtccaga
     6601 ccgacctgga tgcccggtac cgcgacgcca cgctgcaggt taacttgaag attaccaatt
     6661 acacccgccg gccccgcgcg ttattcctcg aggcgaccct gctcgatccg gccggccgcc
     6721 ccgtcggggg cttcggcgcg ccgaagccgg cttatgccga gccgggccgg gaaaccgagg
     6781 cggcccttca tgcccgcgta tccaacccgc tgaaatggag cgcggagacg ccctggctct
     6841 atcagttgct gctcaccctc caggacaacc ggaaacgcgt gctggaggtg attcccgtaa
     6901 aggtcgggtt ccgaaaagtc gagatcaagg acggaaacct gctggtcaac ggccagcgca
     6961 tcctgatcaa gggcgtgaac cggcacgaaa gcgaccccga ctacggccag gccgtcaccg
     7021 tggacagcat gatccgcgat atccacctga tgaagcaata caacttcaac gccgtccgca
     7081 ccgcgcatta cccgaaccag ccggcctggt atgatctctg cgaccgttac ggactgtatt
     7141 tggtggacga agccaacatc gaaagccacg gcatgggcta cggcgcggaa tcgctcgcgc
     7201 gggtcccgga atggcgggcc gcgcacctgg accgcaccct ccggatggtc gagcgcgata
     7261 aaaatcaccc ctcggtgatc atctggtccc tcggcaacga ggccggcaac ggcaccaatt
     7321 tcatggccac ctacgactgg attaaacagc gcgatcccag ccgcccggcg cagtatgagc
     7381 gcgccggctt cgcgcgcaac accgacatca tctgcccgat gtatccccgc ccgagcatgc
     7441 tccggcaata cgccgcgggc gaacgcatcg aaagcaagtg gggggacgaa ttcacgctcc
     7501 cggcgggatc ccggcgcacg cgcccgttca tcatgtgcga atacgcccac gccatgggca
     7561 acagcaccgg caatctctgg ctctactggg atttgatcta tacccagccc ttcctgcaag
     7621 gcgggttcat ctgggattgg gcggaccagg cgctgcgcga gccctgcgcc cgcaacgccg
     7681 cgcgcacggt gcgccggccc aagccgggcg agcccacctt ctgggcgttc ggcggcgatt
     7741 atggcccccc cggcacgccc agcgaccaga acttcctgtg caatggccta gtgacgcccg
     7801 atcgtcggcc gcacccgggc ttgctggaag tgaagcacgt ttaccagtcc atccactgcc
     7861 ggccggttga cctggcgggc ggttcgccgg cctcaaccag cccccggcag ccgcgggtga
     7921 tcgaagtgaa gaacgggttt gatttcctca acctgaaaga cctggcaacg gtaagctggc
     7981 gcctgaccgg ggagggccgg gaactgcaaa agggcgaatt gcccgccccc aatctcccgc
     8041 cccgcgcaac cgcgtccctc gccctcccgg tgaagccctt taagcccgcg ccgggcgtgg
     8101 aatactttct cgaagtcagc ttccgcctga agcatgacga accctgggcc cggcgggggc
     8161 atgagctcgc ctgggagcag ttccaactgc cggagatggc gccggcggtg gcgccggacc
     8221 tggcccgcct cccggcgctg cgcctccgcc aggcgccctc gcaaaccacc gtcaccggcc
     8281 gggactttgc cgccaccttc gacccgcggg ccggcacgct ttgctcgctg aagttcaagg
     8341 gcgtcgagct gatccaggcg ccgccgcgcc ccgacttctg gcgcgccccg acggataacg
     8401 accgcgggcg cgacatggcc caatcgcaag gcatctggcg caccgcgcac accggcgccg
     8461 aggtgcgcgc cttccgcgcc gaggaaaagg cggacacggt tgtcgtgacc atccaccagc
     8521 ggctgccccg ggtggaagcg gattggacca ccacttacac cgtgcatcgg acgggcgata
     8581 tcgaggtgga ggcggcgttc gcccccggcc ggaccaacct gcccccactg ccgcgcctgg
     8641 gcctgcaaat gatcatgccg cccgggtttg accacatcac ctggctgggg cccgggccgc
     8701 aggaaaccta ctgcgaccgc caggacgcgc gcgtcggcct gtactcgggt tcggtgcgcg
     8761 aacaattctt cgcggactac gtcgagccag gggaaagcgg caacaaggtg gaagtccgct
     8821 gggcggcgct gaccaatcgc aagggcctcg gcttgctcgc cgtcgggcag ccgcggctca
     8881 gcgtcaacgc cctccaccac acgcccgacg acctccaggc cgcggcgcat ccgttcgaac
     8941 tgccgcggcg ggatttcgtg gtgctcaacc tggactggcg gcagcagggc ctgggcggcg
     9001 acaacagttg gggctactgg ccgcacgagc cctatctcat cccctgccag gcgcagcgct
     9061 accacttccg cctgcggccc ttcgacgcgg acagcctgcc gggccgaacg gtggcccacg
     9121 tggccggaca gttggcgcgg cagcggattc cgtgagcctc atgccgccgt ggctgaaact
     9181 tgactggcgt gtctttgccg ggctggccct ggccgccgcg gcggtcatct ggtgggggct
     9241 tgatgccctg gaaccgcgcg tcaactggct gcgggtggaa gccccgcgcc gcgcggttgc
     9301 gggacagacc ctgacgctgc gcgttcatct ggccccgctg gcggagtcgt cgcgcgtgtg
     9361 cgccgatctc cattggggaa caacgcgcaa cggcaccctc ggatgcctgg ccagcggcgg
     9421 ctcccaaccc gtcggcccgg agggcggcac ctttgatttc accctccggg tgccctccat
     9481 gaaggaactg caattcgtct ccggggtcat ctacctgagc cggacgggcc agtggcgcga
     9541 ccatacccgg atggcgttca ccgagttcat cccggtgcgc gacggcgcgg gcgcccgggt
     9601 cgagacgcaa ctcaaacccc tgcgcgtgca accgcccgac gaccgcgcgg gccgttcccc
     9661 gcgcgcggcg gcggtccccc gctggctgac cgcgctgctg ttcctggccg gcgccgtgat
     9721 ggcccggcga gccgtggccg cccccgccgc ctccgccgcc ggcgcggaca cccgcttttg
     9781 gcagatcctc gccgtgctgc tgctgctggc cggcctgggg gaactgttcg cggtggagga
     9841 ctggctgggc gcgcgcgccc gggaaatcgc gcgtgcgcat gatctctact acctgcgatc
     9901 cgcgttccag aaaatcagcg tcagcctcgc cggcgcggcg accctcgtct ggctgatcct
     9961 cggcggccgc gcccgccgcc cgcgccggct gctgctgctg gccggcggcc tttacctcgg
    10021 catttccgcg gccaacctcg tatcgctgca cgccgtggac cgtttcgccg acctctcctg
    10081 gcgcggcatc acgctcgtgc aggcgctcaa actcgcctgc gccctcctga ttctgatcgg
    10141 gattcacacc gcccggcggg cggaaaactg accgccgccg gtcgttggcg ttcgagatat
    10201 gcgcgtgatt cgaacctgtc atatcgaagc cgggctgccc tcgctcgaag aggcgcgccg
    10261 gcgggtgctc gaggaaatcc gccgggccaa acgcgagggc gtccgggtgc tcaaagtgat
    10321 tcacggctac ggctcgtccg gcaagggcgg ggcgctgtgc gtcggcctgc gcaaatcctt
    10381 ccggctgcgc cgaaaagagg gggtcatccg ggattttatc cccggtgacg atttttccat
    10441 cttcaccccc caggtgctgg ccctgctgga cgccgtgccc gaattgcgcg gcgacccgga
    10501 tttgaacgcc accaacgagg gcatcaccat tctgtggctg aagtaagccg cttaaaaaag
    10561 cgcccgcgcg ggctggccga atccgctgcc gggggaattt ggccgtgaac agtagcgcgc
    10621 gctcccgtgc tgccgctgct ttgcgggaga gcgcgaaggg ctgcgggcct tgatttttca
    10681 gttatgcaga gatgcaaacc ccggggaaag cccatccttc cggcttcgcg gcgggctgaa
    10741 tcactgcttg cgccggtaaa tcttccaatg cgggtttggc cgttatttat taaccaccaa
    10801 ccccccggcg gccgttcaaa ccgccggggg cattggcttg cggtttatag agccattatc
    10861 ggcacttgat ttggatgacg acccgcgcgg gagcgctgtg gggttggaag gtttgcccgc
    10921 cgtcaaaaga cacccagaag tccagcatgg catccaagtg atagagcaaa acctcgccgg
    10981 gattgggagg cgcgaaggtg gcgctgaaac cgtgaagggc gaggtctttg ataaggatcc
    11041 cgggcgaata gagttccggt tgctccggcg gtgagagata ccggcccatc agcggcggaa
    11101 gactttcacc ggggaactgg tttagcggca ggccgcccgt caggattagc gagacgggtc
    11161 cgttggtggc ggggtgccag ctatggccct ggtcgaggct gatctcggtg aacacgtcaa
    11221 agccgctgct gatggtgcag tggctggtgg cgcttggttt aatcagggtt acgccgcgcg
    11281 aaagcaggga ggggctttcg cggagcatga cgctaaaggg gctggtggcg cccgtggttt
    11341 ccagggtcag cagttcggcg tcgtagattt gccaggcagt cgggttgggg gctgtgaaca
    11401 cacggtagta gcgctcgagg ttggtcgcga ccgtgtcggt gaactgaaac cagcccgccg
    11461 agttggcgag gttggtgccg agggtttgcc aggacagggg gagcgtgagg ctggggacgg
    11521 actggaccac gtagggccag cccgggatgc cggttgcggt gagggccatc accccaccgc
    11581 ccaggttgct gatatcgagg acgctgttgg tcaggtcgtc tccggccagc acgtggaagc
    11641 agatcgcgcc ggtgacgctg gtgtaggggt cggacagggt gtaagtgagc aggtcgccgc
    11701 cgacgtagtc gggtttgggc agccaggcca gggaactggt gccgaccagc gcggcggggc
    11761 cgccgttggt gctcagggcg ctgacgtccg caaggttcag ggggtcgcca tcagggtcca
    11821 ggtcttcggc cagcagcgcc gcgacgggca ggagctgttt ttcattctgg agcacgtaga
    11881 ggatatgcgg gccgggaacg ggtggcgtgt tggcgaggtg gacgacgctg ccggtgctgc
    11941 cgaggaattg gctgttgccc gggtattcgg ccaggattgg ggttaggcca tgcgccaggc
    12001 cggcggtgtc catgagcgcc tggccattgt ccaggggcac gggcggccca agcgggacgc
    12061 cgttggtcgt aaaggtgacg ctgccggtgg gcgtgacgtt cgtttccgcg ttgagcgcgt
    12121 tgacgagggc ccgaagtgtg accgtctcct ccggcagcga gggattcggt cgagtcgtca
    12181 ccgaggtcac ggtgggcacg ggcgtcacca cctcgagacg gatctgattg gtcacgcttt
    12241 catctatcca caccgtgctg ccaggcgccg gggcgccgct caggaaaacg ggggtgggat
    12301 tgaaggcgcc ggttttggcc ccggcataat ggaagagctg ataactgccg tcgcgcaagg
    12361 ggccggcgcc cagcactcga atcgtcacga cttggttatc caccgccagg tcgcctccca
    12421 cccggatcag gtcgttggtg ccgccgctca tggccggcgc gttggtcagc tcgaaaacgg
    12481 tgagcgcgcc gctcgccagg gtcaggttgc ccaggaaggt aagggttccc aggctgtcgc
    12541 ccggttcaag acggccccgc atctggacgg tgacgtcgcc gctcacgctg ccgccgccct
    12601 ggagtgtctg gttggcaagg ccgcccagct cgaacccgcc cgtgaccatg gacacctcca
    12661 gcgacgcgcc gtccgccacc gtgatgaccg ggctgtaggg gatcgccgcc gcatcggcca
    12721 gggcgagcgt gccgcggtgg atgacggtgt ttccgctata ggggctgacg ccggcgccgg
    12781 acaggcgcaa tagtccgctg ccgttcttga ttaaggaact gcccgttccg ttgatggggc
    12841 cgttgaccgc gatggtgccg gcggcgtcgc aggtcaggcc gaacccggcg ctgttgattc
    12901 ccgccagcgc cagttctccg ctggcgcagg agatcgtcgc cgccgccgcc agggtgatcg
    12961 gcccggagaa gcggttgttg ccgctgatgg cgtaaagcgc gccgccgccg tcaccgtttc
    13021 cggcgagcgt caccggctcg gcgctgctgt agttcacgcc gccctgaaag gccagggccg
    13081 cgccgttgct gacgacggtg ccgctgctcg tggcgcccag cgcgttgggg gcggtgataa
    13141 tcagcgtgcc cgccttgacc agcgtcagcc cgccgtaact gttgccggcc cccagcgtgg
    13201 ccgggccggc accttgtttg accagcggac ccgcgccgcc gaggctgcca ccggaaaagc
    13261 ggtaggcggt cgccgtattg ctgatcagca ccgagccggg cgccacgccg cccaccgcga
    13321 tcgtgatgtc cgggtggctg gcgccatcgc ggaaggtgac gaaatcaccg tccccgtagg
    13381 cgcccgggaa accgcccacg tcccaattgc cggccacgtt atcccagatc gcggaggcgg
    13441 cgccactcca gtaaaggttc gcccccgcgt aggcgatctc gaggtaggga ctgtagctgg
    13501 gaaggccgtt ttcccgcgca tgaaacagcc cggcgacgat ggagcccggc ccctcgaagc
    13561 tggattgata cagcaccagg ccgttatttt gctggccgcc gtaccattgg gcgacggaac
    13621 gctggacttc ccaggtgacc caggctggcg gcgcaatggc atccggcggg gtgccgtccg
    13681 cccgcttgat tttgcgcggg ggattgccgt cgtccacctt gtcgttccag gccccatttt
    13741 ggagggtcca cggctgggtc tgcaggtcgt cgcggtacct ccagttgact ccgtggccgg
    13801 aggtgccgtt gtagatgttt tcataccagg acatgccgct gataatgcgg taaggggtga
    13861 tgccaagggc gttatccttt tgcatgctgt agtcgttata gtaataaagc cccagtgtgg
    13921 cgcgggtcag gctctggaag ctgagcgacg gcagggtgaa ttggaccagc gtgcaatcac
    13981 tcatgccgct gttgtattgg acgcggagat aagagcagcc gccgtagttg tccgacttgt
    14041 ggctctcgtc cagccaggca tcggccgcac cggcgtagcc attaattccc ggctgcagtt
    14101 tcaccgttgc cgcgggcagg gaagccgcca cagccaggat caggaatgcg ccggcgaggc
    14161 cggcgcggaa tgcccgggtg ccgcaggccc cgcgggcggg ggaggggagt tttgttttca
    14221 tgggaggttt ggtttcaggt tgttcatgga cggaggcgtg ggcgggcgga tttttggttt
    14281 tgccctcgcc gccagtgagc agggcgcacc cctcctgtag ccaatggagg gccgccctgc
    14341 gtggacgttt tcatactatt tccgcccggc ccagtcttgg cgccgggcac ctgctacaat
    14401 cagttgttat ccgccaaggc gaaatataat cgctgttccc gtgcttgtca agcgggtttc
    14461 agacggttgg ggatgcctca aataaatccc tcgtgccggg gcggacttcg ggctagattt
    14521 gccgggatgt ccacggcgta ttatcaggcg ctagacgcga tcattcagca tgtggaagcg
    14581 ctgcaggagc gcggggttcg ctttatcccg gtcgcgccgg agaccctggc gcggctgcgc
    14641 gaaccgccgc cggttgccgt tcccgccggc ccggcccgat ccgtgccgtc cccgttcccg
    14701 tcggcaactc cggcgcttcc cacggctccg gcggccccgc cgccgcccct ggcggcctcg
    14761 ccgccgttga gtttggaagc ccgggcggcg gctatggccg ggttgcgcca gcgcgtgctg
    14821 gcttgcgtga agtgtccccg gctggcggcg gcgcgccgga acgtggtttt tggcgtgggt
    14881 gccctggacg cccggctcat gttcgtcggc gaagcgccgg gcgccgatga agatgcccag
    14941 ggcgagccgt tcgttgggcg ggcgggtgag ttgcttacca agatcatctg cacaatgggg
    15001 ctgacccggg aggcggtcta tatcgggaat attctcaagt gccgcccgga cacgcccggc
    15061 caggcttccg gcaatcgcaa acccaccccg gaggagatgc agacctgcct tccctatttg
    15121 cacgagcaaa ttgatatcat ccggccggag gtcatcgtcg cgctgggggc gaccgcggtc
    15181 gaggggttgc tgggccggac agccggcatc acccgaatcc gcggccagtg gcatacgtat
    15241 cgcggcacgc cgctgatgcc cacctaccat ccggcttacc tgctgcgcaa aacgtcgctg
    15301 gacgagaaac gccgggtttg ggaggacatg ctgctggtga tggaacgcct tcgcctgccg
    15361 atcagcgaga agcagcgcaa cttcttcctc aaggcactca cgggctgatt ccggcggggg
    15421 actccggggc gtttgttatc ggcgcctgag tttcggtaga ggagggcgaa aagattttcc
    15481 taaccccaaa gccgttgctg tgcggcaaga ccccctctcc gaagcgctta accaccgtca
    15541 acccacaacc gcaccctcct cggttcctga acgataaaac caacgcccct gaacacgttc
    15601 ccgaaccaat cccgccaatc actccccctg cgcgaatagc ctgaggctaa acttccaacc
    15661 aaactcaccc caatcagtcg aatactccgc ccacgatgag ggcaacg
//
LOCUS       DAOPQG010000085        15117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4665_length_15117_cov_19.891847, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000085 DAOPQG010000000
VERSION     DAOPQG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15117
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4665_length_15117_cov_19.891847"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..1531)
                     /locus_tag="PKI07_10065"
     CDS             complement(407..1531)
                     /locus_tag="PKI07_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOC51435.1"
                     /translation="MKIAFGYRTVVVVAALLLGLLLACRTPREIETPAGLAPLPHTNL
                     LVFHNRKGAVVSVKSKADWQQRRAEILRGMTEVMGPLPGPEKWCPFDLRTEQRTNCGS
                     YLRLLLTYASEPGSRVPAYLLVPHQALTGAKKLPAILALHPTDMRYGHRVLVEQLSSS
                     YRAYGRDLAERGYVVIAPAYPLMANYQPDLQALGYQSGTMKAIHDNIRALDLLETLPF
                     VRKGKFGAIGHSLGGHNAIYTAIFEPRIGVVVSSCGFDSFTEYYGGNPDNWQPERGWC
                     QTRYMPRLADYRGRLDEIPFNFHELIAALAPRPLFVNAPLRDANFRQSSVDAILTAAA
                     AVYRLYHVPGVLQAAYPDCEHDFPPEVRETAYNFLDRHLR"
     gene            complement(1545..4052)
                     /locus_tag="PKI07_10070"
     CDS             complement(1545..4052)
                     /locus_tag="PKI07_10070"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOC51436.1"
                     /translation="MKIATLTQWRPRRIARRRRHDPFPGGSRLPLSRPARALRALWRW
                     LPVLLAGMFLPGAGAAGDESRLLKLQAVPFTEVTLQDAFWAPRRETNRLVSIPFSLQK
                     LEEAGNLEDIRLAARGATNGYRGPVFMDSDLYKALEAVAYSLATHPDPALERQLEDII
                     RLLAAAQQPDGYLNSHFIVKDPRGRWTNLRDWHELYCAGHLFEAAVAHYQTTGRTSFL
                     NIATRFADYIDSVFGPPPKRAGYPGHPEIELALVKLGRVTGNPRYFDLARFFLETRGQ
                     KFFAVEHQIPLDKYDGSYFQDDVPIWDHQNIKGHAVRAAYLMSGATDIAAQTGDPRLL
                     RMLDRVWRNTAERNQYLTGGIGPSAHNEGFTVDYDLPNSTAYQETCATIALAQWAHRL
                     ALIYGEARYADALERALYNGMLAGVSRDGTRFFYVNPLESAGTHHRSPWFGCACCPPN
                     VTRTLAALGGYAYAVSTDSLYVNLYIQGTARAKLGETAVALRVDTDYPWDGKVRLELA
                     PAAPTRFALRLRVPGWCPQPSVAVNRRAQPGLLNQRGYLVLDRVWRRGDRVELELPMP
                     VQRIIAHPNVKADLGLAALQRGPIVYCLEQCDHAEPLATLWLPPETQLKPARAPGLLG
                     GVVTLSGEARAVSPQKWERTLYQASAPSRRAALTAIPYCVWDNRQAGPMKVWLPASPP
                     VLPARGLEAAAQVSLSFAHHNCQPQGINDGQQPKNSAEQPAACCHWWPHKGTREWAQY
                     TWPAPVRVSRARVYWFDDTGRGECRLPAAWHLEYLDRGAWKAVAAAESYPVAWDQWCS
                     VRFDPVKTTALRLVVQLPPGFAAGVHEWKVDDPGDVP"
     gene            4252..5535
                     /locus_tag="PKI07_10075"
     CDS             4252..5535
                     /locus_tag="PKI07_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOC51437.1"
                     /translation="MSELVGNLLVAQSGGPTSVINASLAGVIQEAGRNDCIEEIYGGA
                     NGIFGILNEELIDINEERARTIEGLRYTPAAALGTCRYKIDFKKKPEKAAKDMDRLFQ
                     VFEAHNIRYFAYIGGNDSQDTSHKIHEEAIKRNYVLRVLGVPKTIDNDLPHTDNCPGY
                     GSVIKYNAITVTEVGLDVGSMATDDGSCCIIEVMGRSAGWIAAGTVLAKRGNPANPPH
                     IILLPELPLEEEKFLAKVKETVAAYNYCVVVVGEGIKDTSGKEVGADKTRLDAFGHPV
                     LAGAAQKLKELVQGKLNTKTRTVLLGYAQRAAAHCASLTDATNAFACGEAAVKAAIAG
                     ESGYMVKIVRTPQPDGSVQWATGLQPLGDIANVEHFIPRDWISEDGYLPNEKFVAYAQ
                     PLIEGEVKCPTEGGLPKYVVLEKTKVEKKLPPREG"
     gene            5682..7043
                     /locus_tag="PKI07_10080"
     CDS             5682..7043
                     /locus_tag="PKI07_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51438.1"
                     /translation="MNPPGADAPSFPPGLHHAYLFSIFNAFSYQIVLNSPMVLYAKTL
                     GASATVLGIIAGMMPLLVIFQIPAASYIPRLGFKRFVYAGWGTRVMFIFGMALVPLTG
                     FFLKPETRLALLLTLLFGFNLSRGISSCAWLPWITALVPAPLRGKYLARDSAVQSCAS
                     LLTFLIAGACLSGPTHAWQFAILFAFSAIMGAVSLSFLKRIPDAEMPQEPGAAKGRVP
                     WREMIRYDPFRRLLCALVGYSVASGGLTAFVVAFLKSETGMSEAQILLLSSSAYLGGL
                     SSLWLLGSRLDHLGSKPVLAFCHTGWVAVLGGWICLAGGLFPVRLTSVLVLQYLMGLL
                     VALVQMANTRLAMAIIPVMGRNHFFAIYSTIGNVTLGLAPIGWGLLIDAVGHRSPHWL
                     GLTWGRYTVFFAAAALAFAITLALARRLHEPEAASMEDLLREILIESPQRFWIRFWPR
                     GGA"
     gene            complement(7069..9051)
                     /locus_tag="PKI07_10085"
     CDS             complement(7069..9051)
                     /locus_tag="PKI07_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOC51439.1"
                     /translation="MKTTDAGTAEPTWRESRYGFAGVCFLSLLAAWSLLRLILLIAFK
                     PPATPGAEFLLVLLGSLHRDAFAALVETLPLLFWLLLVPNSRFGARWHKVVLLGGWGV
                     FCFLQSFLLFVEFFFFEEFKSRFNTVAVDYLLFPKEVFINIWASYHVGVVLTVCLALS
                     LGAVFAVHRGFGRMWQKPFSLKSRLLHLAAGLILAALLVPTFDLKGAHVSRDRTLNEI
                     ANNGALSFVAAAWTRNLDYRAFYRTMPLDEAYQRARRLLAAPNAQFVETGSSIRRQVA
                     GDPARPRLNVVILLEESLGSTFWGCLGRTNTLTPEMDKLATEEGMLFTHIYATGNRTV
                     RGFEGVLSSFPPLPGESIVKRDRSDNVETIARVLKRDGYTNVFLYGGRGLFDSMRSFA
                     RRNGYDRFIEQKHFAQPTFTTIWGVCDEDLFQRAIVEFRELARTGQPFCGTVLTVSNH
                     KPYTYPKGRIPENPDERRRRHAVKYADYALGQFFQAARQEAFWTNTLFVVVADHGARV
                     YGRQSIPIRSYEIPLLIAGPAVVKHPSRIAQLGCSLDVPTTILGLIGRPYESLFFGRD
                     LRLRPDEEGRAWVNHNRDIGLLTHDRLVVLGLKQTVEFYQGNPKVVDMTLLPQPTAAD
                     RELEKDAIAIYQVADDLYMNRRYRIDDTTAPPDPER"
     gene            complement(9143..10090)
                     /gene="fmt"
                     /locus_tag="PKI07_10090"
     CDS             complement(9143..10090)
                     /gene="fmt"
                     /locus_tag="PKI07_10090"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOC51440.1"
                     /translation="MAALRIVFMGTAELSCDSLRGLLAAPGFQVAAVVTQPDRPKGRD
                     LRLQPSPVKELGLQAGLPVLQPERARSEAFLAELRGLQPELIAVVAYGQILPPDILSL
                     PRFGCLNVHTSLLPRYRGAAPIQWAILNGETETGVTIMKMDAGLDTGGILTQARTPIQ
                     PEDTAETLRERLARMGAELLARTIPDYIAGGLVPREQPAAGVSYAPKIRKQDGRIDWQ
                     QPARAIWNRVRGLVPWPGAFSFLPGQPQPHLLKIWQAEVVEDSGAPGVVLRADRNGIV
                     VGCGEAALRIRVLQREGGRRLAAADFLAGQRLSPGQALG"
     gene            complement(10162..14673)
                     /locus_tag="PKI07_10095"
     CDS             complement(10162..14673)
                     /locus_tag="PKI07_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOC51441.1"
                     /translation="MKKTEQLPARPSFEPQRYNFIPEHRLARLAVLVLWASVLLVTAD
                     SPGATYSWSGGGTTGNWSESANWGYAGVPGSADILIFPAGALKLTNTNNLPGLSVSQI
                     RFAGAGGGYSIWGNPLTLNGSILATNSAGSNVINNALTLGAPAVSVEVNIGARLTLGG
                     TVSGSGGLTKTGRGTLVLAGSGNNSYIGATRVSEGMLELNKSGASINAGSLTISNATV
                     KEMSMTQIGEIPVTVDAGGILDLNGFGDTIGNSLTLNDGKVMTGTGALWMLSDSTITL
                     SGVSSISGNLHLLGGTCTFTGSGVTTLYANISGGVSLLKTGSVNLELQGTNTFSGALT
                     VNGSGYVYALHPQAFGTSTNGVFINDATYLGLYSAAITNKALTLNTTYNFSMIASGAG
                     RSIWAGPISLSRDAQVYVATNSLLEVHGAITGAGGLRKWGPGTLVLAGATANTYAGVT
                     TVNEGQLELDKTIPAGAIPPAGLVIGDGIGTDTVRCRRNYQLWTLGKPVTINRSGVLN
                     LNGFHDTAIPLTLEGGALTTGTGTIHLGNTLTVRPDTQMATIQGNASLMQALVITNTG
                     HSALYDLTISAAISGIHGLTKTGAGAVRLSASNSFTGPVTINQGLLSSWNADALGNTN
                     TPVTVNSGGTLHVGSNVTFAPKPLVLNGPGWSMGSFRGALAASDGGCVWTGVITNASD
                     SVIHVYGSRSLEINGPIRGPGGLTKSGAGTNIFSGPLPNTYAGVTRVDDGTLILNKPG
                     GVKSIPGNLEIGGGIVGGPSVRLGNVNQIASTAEVFIDAGGLLECGANSALIGALRGS
                     GTVNFGAGGYLVIMQGGGTCIFDGTMTGIGYAPGYTLGKSGGGTFIMNGDANFSAGNI
                     QVFGGKLVVNGVTARPAFVGTGTTLGGHGTVGAITNYGAIAPGSSAGKLNSGNLSFGT
                     NGSLAIELNGNAAGSGYDQLNVAGGVNLTGAVLAPLTVGFLPRQGDRFVIINNDGSDP
                     VAGTFTGLAEGAVISDSTGKFHFRVSYVGGSGNDVELTMTNQSLAGGAAYVLSGNGNG
                     VLDPNECNLLNLTISNRNAAAMSGISTTLRSLTHGVAVVQPYSTYPGIPAYQARTNNA
                     FFQVTTQPGFVCGAPVNLELQVATASHGILTVPFSLPSGAVGAAARFDNNTSSAIPDV
                     GYIDKTLWVSGITTPLKKVWVSLHIVHPVVGSLDLSLRGPDGTTVALSSGHGGSKPNY
                     GTSCVDRSRTTFDSDAATPIAMGTPPFVGVYQPESSLAAFYGKSGTNVNGEWRLRVAD
                     NFPGATGSILCCSLFLAPTACAPGGGICELCPDATIQSYIGAETPLQAGYLQANGIAS
                     ACGSVKACPPLGSGSYPAENFTFRNGPSNACITVVASHSDPIAALVAAAYLDSFDPAA
                     GLCVNYLGDAGFYVNAAHPSQAFSFQVPAYAVFIINVVSDSIGMPYTLAVSGGDCRPL
                     LNISRVGASRMALDWTTAAPGYRLEAASQLNGAPWETVPPYPPRVVNSRFAVSNAMEN
                     LKFYRLHKP"
BASE COUNT         2715 a         4733 c         4922 g         2747 t
ORIGIN      
        1 ccccagtggc ttaccatgac ccgctgcttt ttgcagaact tttaggacac taccataaaa
       61 cgctcaaata acccggcatt ttggctccca ataggtcgtg tacaaggggt gtacaaggga
      121 tgcccaccgg gtgtaccagc taaaatccta tgaactcccg gtgagcatcc cttgtacacc
      181 ccttgtacac ggcgctttgg cagggggaaa tcggggaaat gggcgggaac gggaagcggg
      241 gggctataaa agcgttggcg ggaggattat tgatgtttct ggggtgaacc caaggggaaa
      301 tcgtattaaa gaagcagccg ggcgttatcg ttgcagaagc ccggttggag ccgggattaa
      361 cccgattttc actgtttggc gattagtttt ccctggggcg agggggtcag cgcaggtgcc
      421 gatcgaggaa gttgtaggca gtttcgcgca cttcgggggg aaaatcgtgc tcgcaatcgg
      481 gataggcggc ctggagcacc ccgggcacgt ggtatagccg atagacggcg gcggcggcag
      541 tcaggatggc gtccacgctc gattgacgga agttggcgtc gcggaggggg gcgttgacga
      601 acaggggccg gggggcgagg gcggcgatga gttcatggaa gttgaagggg atttcgtcca
      661 ggcgcccgcg gtaatcggcc aggcggggca tatagcgggt ttggcaccaa ccgcgctcgg
      721 gttgccagtt gtccgggttg ccgccgtagt attcggtgaa ggagtcgaac ccgcaactgg
      781 agacgaccac gccgatgcgc ggctcgaaga tggcggtgta gatggcgttg tgtccgccca
      841 gggaatggcc aatggcgccg aactttcctt tccgcacgaa cgggagtgtt tccagcaggt
      901 ccagggcgcg gatattatca tggatggcct tcatggtgcc gctttggtag ccgagcgcct
      961 ggaggtcggg ctggtaattg gccatgagcg ggtaggcggg ggcgatgacg acgtagccgc
     1021 gttcggccag gtcgcggccg taagcgcggt agctgctgct taattgctcg acgagcacgc
     1081 gatggccgta gcgcatatcg gtggggtgca gcgccaggat ggcgggcagt ttcttagcgc
     1141 cggtcaaggc ctggtggggc accagcaggt aggcggggac gcgcgagcct ggctccgacg
     1201 cataggtcag caagaggcgc aggtaggagc cgcaattggt tcgctgttcg gtgcgcaagt
     1261 cgaaggggca ccatttttcc ggtcccggca gcggccccat cacttcggtc atgccgcgca
     1321 ggatctccgc gcggcgctgc tgccagtcgg ccttggattt gacggagacg acggcgccct
     1381 tgcggttgtg aaagaccagc aggttggtgt gcggcagcgg cgcgaggccg gcgggcgttt
     1441 ctatttcgcg gggggtgcgg caggccagca ggaggcccag aaggagggcg gcaaccacca
     1501 cgacggttcg atagccgaaa gcgattttca tagggcaggc tgaattaagg aacatccccg
     1561 ggatcgtcca ccttccattc atgcacgccg gcggcgaagc cgggagggag ctggaccacc
     1621 aggcgcaagg ccgttgtttt tacgggatca aagcgcacgg agcaccattg gtcccaggcc
     1681 acgggatagg attccgcggc ggcgacggct ttccatgcgc cccggtcgag gtattcgaga
     1741 tgccacgcgg cgggcagccg gcattcgccg cggccggtat catcgaacca atatacccgg
     1801 gcgcggctga cccgcaccgg ggccggccag gtgtattggg cccattcgcg ggtgcctttg
     1861 tggggccacc aatggcagca ggcggcgggt tgttcggcgc tgttcttcgg ctgctggccg
     1921 tcgttgatcc cctgcggctg gcagttatgg tgggcaaagc tcaggctgac ctgcgcggcg
     1981 gcctccagtc cgcgagctgg caacaccggc ggcgaggctg gcaaccaaac tttcatcggt
     2041 ccggcctgcc ggttgtccca aacgcaatac ggaatcgccg tgagggcggc gcggcgcgag
     2101 ggcgcggagg cctggtaaag ggtgcgctcc catttttgcg gagaaaccgc gcgcgcctcg
     2161 ccggacaggg tcaccacgcc gcccagcaag ccgggcgcgc gcgcgggttt gagctgcgtt
     2221 tccggcggca gccacagggt ggcgagcggc tcggcgtgat cgcattgttc caggcaatag
     2281 acgatcggcc cgcgctgaag ggcggccagc ccgaggtcgg ctttcacgtt cgggtgggcg
     2341 atgatccgct ggacgggcat ggggagctcc agctcgacgc ggtcgccgcg ccgccatacg
     2401 cggtcgagaa cgaggtagcc gcgctggttg agcaaccccg gctgggcgcg gcggttgacg
     2461 gcgaccgagg gctgcgggca ccagccgggc acgcgcagcc ggagggcgaa gcgggttggg
     2521 gcggcggggg ccagttccag ccgcactttg ccgtcccacg gatagtcggt gtccacccgc
     2581 aacgcgacgg cggtctcgcc gagtttcgcc cgggcggttc cctggatata gaggttgacg
     2641 tagagcgagt cggtgctgac ggcgtatgcg tagccgccga gggcggcgag ggtgcgggtc
     2701 acgttgggcg ggcagcaggc gcagccgaac cacggcgagc gatggtgggt gccggcgctt
     2761 tccagcgggt tgacgtagaa gaatctcgtg ccgtcccggg agacgcccgc gagcatgccg
     2821 ttgtagagcg cgcgttccag ggcatcggcg tagcgcgcct cgccgtagat cagggccagg
     2881 cggtgcgccc actgggcgag ggcgatcgtc gcgcaggttt cctggtaggc ggttgagttg
     2941 ggcaggtcgt aatccacggt gaagccctcg ttgtgggcgc tggggccgat gccgccggtg
     3001 aggtattggt tgcgttcggc ggtgttgcgc cagacgcggt ccagcatgcg gagcaggcgc
     3061 gggtcgccgg tttgcgccgc gatgtccgtg gccccggaca ttaggtaggc ggcgcggacg
     3121 gcgtggccct tgatgttttg gtggtcccaa atggggacat cgtcctggaa ataggagccg
     3181 tcatacttgt ccagcgggat ttggtgttcg acggcaaaga atttctggcc ccgggtttcg
     3241 aggaagaagc gcgccaggtc gaaataccgc gggttgccgg tgacgcgccc gagcttgacc
     3301 agggccagct cgatttccgg gtggcccggg tagcccgccc gtttgggcgg cgggccgaaa
     3361 accgagtcaa tgtagtccgc aaaccgggtg gcgatattca ggaaggaggt tcggccggtg
     3421 gtttggtagt gcgcgacggc cgcttcgaac aggtgcccgg cgcaatagag ttcgtgccag
     3481 tcgcgcaagt tcgtccatcg cccgcggggg tccttgacga tgaaatggct gttgaggtag
     3541 ccgtcgggtt gctgcgcggc ggcgagcagg cggatgatgt cctccagttg ccgttcgagc
     3601 gcggggtcgg ggtgggtggc gagcgagtag gccaccgctt ccagcgcttt gtagaggtcc
     3661 gagtccatga acaccgggcc gcggtagccg ttggtcgcgc cccgggcggc cagccggatg
     3721 tcttcgaggt tgcccgcctc ttcgagtttc tggaggctga aggggatgct gaccagccgg
     3781 ttcgtttcac gccgcggcgc ccagaacgcg tcctggaggg tgacctctgt gaacggcacc
     3841 gcctggagtt tgagcaagcg ggattcgtct ccggcggccc cggccccggg caggaacatt
     3901 ccggccagga gcaccggcag ccaacgccaa agcgcccgaa gcgcccgggc cgggcgggag
     3961 agcgggagcc gggagccgcc ggggaacggg tcgtgccgcc gccgccgggc tatccgccgg
     4021 gggcgccatt gggtgagcgt cgcgattttc attttcgcgg ctcatggtcg gccccgggat
     4081 gaatctgtca accctgtttc gggcggcggc gggagttttc gagcggagcg cggtcgaatt
     4141 gggctttgac agggcggcgc attattggca cgctagggat tgaattcgga tgggattcca
     4201 aacatcaggg ttgacggagc tcagcccggc aaaacagtaa tttgtataaa tatgtcagaa
     4261 ctagttggaa acttgttagt ggcgcaatcc ggcgggccga cgtccgtcat caacgccagt
     4321 ttggccggtg tgattcagga agcgggccgg aatgactgta ttgaggaaat ctacgggggc
     4381 gccaatggca tctttggcat cctcaacgag gaactgatag acattaacga ggagcgcgcc
     4441 cggaccatag agggcctcag atacacgccc gccgcggcgc tcggcacgtg ccgctacaag
     4501 attgacttca agaagaagcc cgagaaagcc gccaaagaca tggaccggct cttccaggtg
     4561 tttgaagccc acaacatccg ctacttcgcc tacatcggcg gcaacgactc ccaggacacg
     4621 tcgcacaaga ttcacgagga agccatcaag cgaaattacg tgctccgcgt cctcggcgtg
     4681 cccaagacca ttgacaacga cctgccgcac acggacaact gcccaggcta tggctcggtt
     4741 atcaagtaca acgccatcac agtcaccgaa gttggcttgg acgtgggcag catggcgacc
     4801 gacgacggct cctgctgcat cattgaagtg atgggccgtt cggcaggctg gatcgccgcc
     4861 ggcaccgtcc tggccaagcg cggcaacccc gctaacccgc cccatatcat cctgctgccc
     4921 gaactgcctc tcgaggagga gaagttcctc gccaaggtaa aagaaaccgt agccgcctat
     4981 aattactgcg tcgtagtcgt tggggaaggg atcaaagaca cctcggggaa ggaagtgggc
     5041 gccgacaaga cccggctgga tgcgtttggc cacccggtgc ttgcgggagc ggcccagaag
     5101 ttgaaagaat tggtgcaggg caagctcaat accaagaccc gcaccgtgct gctcggctac
     5161 gcgcagcgtg ccgccgcgca ttgcgcgagc ttgaccgatg ccaccaacgc ctttgcctgc
     5221 ggcgaagccg ccgtcaaggc cgccatcgcc ggcgagagcg gctacatggt caaaatcgtc
     5281 cggaccccgc agcctgacgg ctcggttcag tgggccacag gcctgcagcc gctgggcgac
     5341 atcgccaacg tcgaacactt cattccgcgc gactggatca gcgaagacgg ttacctgccc
     5401 aacgagaagt tcgttgccta cgctcagccg ttgatcgaag gcgaagtcaa gtgcccgaca
     5461 gaaggcggcc tgcccaagta cgtggtgttg gagaagacca aggtagagaa gaagctcccg
     5521 ccgcgcgagg gctgaaatag ccctgcggtt ctgatttccc agagcgggga tgcctcgccg
     5581 cggcatccgc ttttgattcg catggccgcg caactcgggc catgtttggt ccgattgaga
     5641 gactgagctc gggcattatg gccaggcacc acacagacag cttgaacccg ccaggagcgg
     5701 acgcgccctc cttccctccc ggcctgcacc atgcctatct gttctccatc ttcaatgcat
     5761 tctcctacca gattgtgctc aacagcccga tggtcctcta cgccaagacc ctcggcgcca
     5821 gcgccaccgt gctgggcatc atcgcgggga tgatgcccct gctggtcatc ttccaaatcc
     5881 ccgcggccag ctacattcca cgcctgggct tcaagcgctt tgtctatgcg ggctggggca
     5941 cccgggtgat gttcatattt ggcatggcgc tggtgccttt gaccggtttc tttctcaagc
     6001 cggaaacccg gctggcgttg ctgctcacgc tgctgtttgg tttcaatctg tcgcgcggca
     6061 tctccagttg cgcctggctg ccgtggatca ccgccctggt tccggcgcct ttgcgcggca
     6121 agtatctcgc gcgcgattcc gccgtccaaa gctgcgccag cctcctcacc ttcctgatcg
     6181 ccggggcctg cctcagcggg ccgacgcacg cctggcagtt cgccattcta ttcgccttta
     6241 gcgccatcat gggggcggtg agcctttctt tcctcaagcg cattcccgat gccgaaatgc
     6301 cgcaggaacc cggagccgcc aaggggcggg tgccgtggcg ggagatgatc cgctacgacc
     6361 cgttccggcg gctgctttgc gccctggtcg gctactcggt ggcttcgggc gggctgacgg
     6421 cgtttgtcgt ggctttcctg aagagcgaga cggggatgtc cgaggcgcag atactgctgc
     6481 tcagctccag cgcctacctc ggcggcctca gcagtctctg gctgctgggc tcgcgcctgg
     6541 accacctcgg cagcaaaccc gttttggcgt tttgccatac cggctgggtg gcggtgctgg
     6601 ggggatggat ctgcctggct ggcgggcttt tccccgtgag gctgacgagc gtcctggtgc
     6661 tgcagtatct gatgggcctg ctggtggcgc tggttcagat ggccaatacc cgcctggcga
     6721 tggccatcat cccggtcatg gggcgcaacc actttttcgc catctattcc accatcggca
     6781 atgtcaccct cggccttgcg ccgatcggct ggggattgct gattgatgcc gtgggccatc
     6841 gcagccccca ttggctggga ttgacctggg gccgctacac cgtcttcttt gctgccgccg
     6901 cccttgcctt tgccatcacc ctggccctgg cccggcggct ccacgagccg gaggccgcca
     6961 gcatggaaga cctcctgcgc gaaatcctga ttgaatcccc gcagcgcttc tggattcgtt
     7021 tctggccgcg gggcggggcg tgaacggttt tccgggcgcg ccgccggctc aacgctcggg
     7081 gtcggggggc gccgtcgtgt cgtcaatccg gtagcgccgg ttcatgtaga gatcatccgc
     7141 gacctggtaa atggcgatgg cgtccttttc gagttcgcgg tcggcggcgg tgggctgcgg
     7201 gagcaatgtc atatccacaa ccttcgggtt gccctggtag aactcgactg tttgcttcaa
     7261 gcccagcacc accagccgat cgtgcgtcag caggccaatg tcccggttgt gattgaccca
     7321 ggcgcggccc tcctcgtccg ggcggagccg caggtcgcgt ccaaagaaca ggctttcata
     7381 cggccgcccg atcagcccca ggatggtggt gggcacatcc agcgagcagc cgagttgggc
     7441 gatgcggctc gggtgtttga ccaccgccgg cccggcgatc agcagcggga tttcatacga
     7501 ccggatcggg atgctctgcc ggccatacac gcgcgcgccg tgatccgcca ccacgacaaa
     7561 aagggtattc gtccagaacg cctcctgccg ggcggcctgg aagaactggc ccagggcgta
     7621 gtcggcgtat ttcaccgcgt ggcggcgccg ccgttcgtcc gggttttcgg ggattcggcc
     7681 tttcggatag gtgtacggct tgtggttgga aacggtcagc accgtgccgc agaacggctg
     7741 gccggtccgc gccagttcac gaaactcgac gatggcgcgc tggaacaggt cttcgtcgca
     7801 gacgccccaa atcgtggtga aggtcggctg cgcgaaatgc ttctgctcga tgaaccggtc
     7861 gtagccgttg cgccgggcaa acgagcgcat gctgtcgaac aggccgcgcc cgccatagag
     7921 aaacacgttg gtgtagccat cccgcttcag cacccgggca atcgtctcca cattgtccga
     7981 gcggtcgcgt ttgacaatgg actcccccgg cagcggcgga aaggacgaca gcaccccctc
     8041 gaacccgcgc accgtccgat tgccggtcgc atagatatgg gtgaacaaca tgccctcttc
     8101 cgtggccagt ttatccatct ccggcgtgag cgtgttcgtc cgccccaggc agccccaaaa
     8161 cgtggacccc aggctttctt cgagcagaat taccacgttc aaccgcgggc gggccgggtc
     8221 gccggccacc tgccgccgga tggaggatcc ggtttcgaca aattgcgcgt tcggtgcggc
     8281 cagcagacgg cgcgcccgct ggtaggcttc gtccaggggc atggttcggt agaacgcccg
     8341 gtaatcgagg ttgcgcgtcc acgccgcggc cacgaaggac agcgcgccgt tgttggcgat
     8401 ttcgttcaaa gtgcggtccc ggctcacatg cgcgcctttg agatcgaaag tgggcaccag
     8461 cagggcggcc aggatcaggc ccgccgccag gtgcagcagc cgggatttca aggaaaacgg
     8521 tttctgccac atccggccaa acccgcggtg gacggcgaac accgcgccca ggctcaaagc
     8581 caggcaaacc gtcaggacga cgccgacatg gtacgacgcc caaatgttga taaagacttc
     8641 cttgggaaag agcaggtaat ccaccgcaac ggtattaaag cgcgacttga attcctcgaa
     8701 gaagaagaac tcgacgaaca gcagaaagct ctgcaaaaag cagaagactc cccagcctcc
     8761 caggaggacg accttatgcc accgggcccc gaaccgggag ttaggcacga gcagcagcca
     8821 gaacaacagg ggcagtgtct ccaccagcgc cgcaaaggcg tcgcgatgca ggctgcccag
     8881 cagcaccagc aggaactccg ccccgggcgt cgcgggcggt ttgaaggcga tgagcaggat
     8941 caaccgcagc agggaccagg cggccagcag cgacagaaaa cagaccccgg caaacccgta
     9001 gcgcgattcg cgccaggtcg gttcggctgt gcctgcatca gtggtcttca tatttgaacc
     9061 gattctaccc ccgaacggcg ccagtgcaat ccgccgtttt cggcagggtg gcaggaacgc
     9121 cgcccggacg gggagcccgc catcagccca gcgcctggcc cggcgataat cgctggcccg
     9181 ccaaaaaatc cgccgcggcc aggcgccggc cgccttcgcg ctgcaagacc cgtattcgca
     9241 gggcggcttc cccgcacccc acgacaatgc cattcctgtc cgcccgcagc accacgccgg
     9301 gcgcgcccga atcctccacg acttccgcct gccagatttt gagcagatgc ggctgcggct
     9361 ggccgggcaa aaagctgaac gcccccggcc agggcaccag cccccgcacc cggttccaga
     9421 tcgcccgcgc cggctgctgc cagtcaatcc ggccgtcttg tttccggatt ttgggcgcgt
     9481 agctgacgcc cgccgccggt tgctcccggg gaacaaggcc gcccgcgata tagtccggga
     9541 tggtccgcgc cagcaactcc gcccccatcc gggcgagtcg ctcgcgcaaa gtctccgcgg
     9601 tgtcctccgg ctgaatcggc gtccgggcct gcgtcaaaat gcccccggta tccaggcccg
     9661 cgtccatctt cataatcgtg acgcccgttt ccgtctcgcc gttgagaatc gcccattgga
     9721 tcggcgcggc gccgcgatac cggggcaaca gcgaagtatg aacattgagg cagccaaagc
     9781 gcggcaggct cagaatgtcc ggcggcaata tctggccata ggccaccacg gcgatcagct
     9841 ccggctgcag cccgcgcagc tccgccaaaa acgcttcgct gcgggcccgc tcgggttgca
     9901 gcaccggcag cccggcctgc agccccagtt ctttcaccgg cgaaggctgc agccggaggt
     9961 cccgcccttt gggccggtcc ggctgcgtga cgacggccgc cacctgaaag cccggcgcgg
    10021 ccagcagccc gcgcaggctg tcgcaggaca gttccgccgt gcccatgaag acgattcgca
    10081 atgcggccat gcgtcaaggc tagccgcctc cgcgcggatt acgagccgaa tcggcctggc
    10141 cggccggggg tgcgcaagcg cctacggttt atgcaagcgg taaaacttga ggttttccat
    10201 ggcgttgctc accgcaaacc ggctgttcac cacgcgcggc gggtagggcg ggaccgtctc
    10261 ccacggggcg ccgttcaact ggctggcggc ctccaaacga tagccgggcg ccgcggtggt
    10321 ccagtccagg gccatgcggg aggcgccaac gcgtgagatg ttcagcagcg gccggcagtc
    10381 gccaccgctg acggccaggg tgtaaggcat gccgatgctg tccgagacca cattgatgat
    10441 gaagaccgcg taggccggca cctgaaagga aaaggcctgg ctcggatgcg cggcattcac
    10501 gtaaaagccc gcatcgccca gataattgac gcacaagccg gcggccggat caaagctgtc
    10561 caggtaggcc gccgcgacca gtgccgcgat ggggtcggaa tgcgacgcga ccacagtgat
    10621 gcaggcatta ctggggccat tccggaacgt gaagttttcg gcgggataac tccccgaccc
    10681 cagcggcggg caggccttca ccgaaccgca ggcgctggcg atgccattgg cctgcaagta
    10741 cccagcctgc aacggagttt cagcgccgat gtaggattgg atggttgcgt cggggcacaa
    10801 ctcgcagatg ccgccgcccg gcgcgcaggc ggtgggggcg aggaagagcg agcagcagag
    10861 aatactgccc gtcgcgccgg gaaaattgtc cgcgacgcgc aaccgccact cgccgttgac
    10921 gttggtgccc gatttcccgt agaaggcggc cagagagctt tccggctggt aaaccccgac
    10981 aaacggcggc gtccccatgg caatgggcgt cgccgcgtca ctatcaaagg tggtgcggct
    11041 tctatccacg caagaggtgc cgtaattcgg cttgctgccg ccatggccgc tggacagagc
    11101 caccgtggtg ccatccggcc cgcgcagcga aagatccagg ctgccgacga ccgggtggac
    11161 gatgtgcagc gatacccaca ccttcttaag cggcgtggta atgccgctca cccagagggt
    11221 tttgtcgata tagccaacgt cggggatggc gctcgacgtg ttgttgtcga atcgcgcggc
    11281 cgcgcccacc gctccgctgg gcagcgagaa cggcaccgtc aatatgccgt gggaggcggt
    11341 ggccacctgc agttccagat ttaccggggc gccgcagaca aagccgggct gcgtggtgac
    11401 ctggaagaac gcgttattcg tgcgcgcctg gtaggcggga atgcccggat aggtcgagta
    11461 cggctgcacc acggccacgc cgtgggtgag cgaccgcagg gtggtgctta tcccgctcat
    11521 cgcggcggcg ttgcgattgg agatcgtcag gttcaataaa ttgcactcgt tcggatcgag
    11581 gacgccgttg ccattgccgc tcaacacgta ggcggcgccg cccgccagcg actggttggt
    11641 catcgtcagc tccacgtcgt tgccgctgcc gccgacgtag ctgacgcgga aatggaactt
    11701 gcccgtgcta tcgctgatca ccgcgccttc cgccaggccg gtaaaggtgc ccgccaccgg
    11761 gtcggagccg tcgttattga tgataacgaa ccggtcgccc tgccggggca ggaagcccac
    11821 cgtcagcggc gccaggactg ctcccgtcaa atttacgccg cccgccacgt tcagttggtc
    11881 atagccgctg cccgcggcgt tgccgttcag ctcgatggcc aggctgccat tggtgccaaa
    11941 gctgagattg cccgaattaa gtttgccggc actggatccc ggagcgatgg cgccgtagtt
    12001 cgtaatcgcc cccaccgtgc cgtggccgcc gagcgtggtg cccgtcccca cgaaggccgg
    12061 ccgggcggtg acaccgttga ccaccagctt gccgccaaac acctggatat tcccggcgga
    12121 gaaattggcg tcgccgttca tgatgaatgt gccccctccc gacttgccca gcgtatagcc
    12181 gggggcatac ccgatgcccg tcatcgttcc gtcaaagatg caggtgccgc cgccctgcat
    12241 gatcaccagg tagcctcccg ccccaaagtt cacggttccg gagccgcgca aggcgccaat
    12301 caacgcggaa ttcgcgccgc actcgagcaa accgccggca tcaataaaga cctccgccgt
    12361 ggacgcgatc tggttgacgt ttcccagccg tacggacggt ccgccgacga tgccgccgcc
    12421 aatctccaga ttccccggta tggatttcac gccgccgggc ttattcagga tcagggtgcc
    12481 gtcatccacg cgcgtgacgc cggcgtaggt gttggggagc gggccggaga agatgttggt
    12541 gcccgcgccg ctcttggtca gacctccggg gccgcggatg gggccgttaa tctcaaggga
    12601 gcgggatcca tagacgtgga taacgctgtc gctggcgttg gtaatcacac ccgtccagac
    12661 gcagcccccg tcgctggcgg ccagagcccc cctaaaactg cccatgctcc aacccgggcc
    12721 attcaggacc agcggtttgg gggcgaaggt cacgttgctc ccgacatgca gcgtgccgcc
    12781 actgttgacg gtgaccggag tattggtgtt gcccaacgcg tcggcattcc aactggaaag
    12841 caagccctgg ttgatcgtga ccgggccggt gaagctgttg gaggcgctca agcgcaccgc
    12901 gccggctccc gtcttggtca aaccgtgaat gccgctaatc gcggcgctaa tagtcagatc
    12961 gtaaagcgca gaatgcccgg tattggtgat gaccagtgcc tgcatcaacg atgcgttccc
    13021 ctggatggtg gccatttggg tatcgggccg cacggtcaac gtgttgccca ggtgaatggt
    13081 gcccgtcccg gtcgtaagcg ctcccccttc cagcgtgagc gggatggcgg tatcatgaaa
    13141 accattcagg ttcagcactc ccgagcggtt gatggtgacc ggcttcccga gggtccaaag
    13201 ttgatagttc ctccggcagc ggacggtgtc cgtgccaatg ccatctccga tgaccaaacc
    13261 ggccggggga atcgcccccg ctggaatggt cttgtcgagt tcaagctgcc cttcattgac
    13321 cgtggtgacc ccggcgtagg tgttggcggt ggcgccggcc aggaccagcg tgcccggacc
    13381 ccatttgcga agccctcccg cgccggtgat cgcgccgtgg acttccagca agctgttggt
    13441 cgccacataa acctgcgcgt cgcggctcag gcttatcggc ccggcccaaa tgctgcggcc
    13501 cgcccccgac gcgatcattg aaaagttgta ggtcgtgttc agcgtcaacg ccttgttggt
    13561 gatggccgcg ctgtacaacc ccaggtaagt cgcatcattg atgaaaaccc cgttggtgga
    13621 ggtgccaaac gcttgcggat gcaacgcgta aacatatccg ctcccattga ccgttagcgc
    13681 gccgctgaac gtgttcgtgc cctggagctc caggtttacg ctgccggttt ttagcaggct
    13741 gacccccccg ctgatgttcg catagagagt cgtcacaccg gagcccgtga aagtacaggt
    13801 gccgcccagc aggtgcagat tcccggaaat cgaggacacc ccgcttagcg tgatggtgga
    13861 atcgctcaac atccacagcg cgcccgttcc cgtcatgacc ttcccatcgt tcagcgtcag
    13921 gctgttgcca atcgtatccc caaacccgtt cagatccagg atgccgcccg cgtccaccgt
    13981 cacgggaatc tcgcctattt gcgtcatgga catctccttc acggtggcgt tggaaatcgt
    14041 cagcgaaccc gcgttgatgc tggccccgct tttgttcaat tccagcattc cctcgctgac
    14101 ccgggtcgcg ccgatatatg aattgttgcc cgaccccgcg agcaccaggg tgccccggcc
    14161 cgtcttggtc aagccgccgc tcccgctcac cgtgcctccc agcgtcaggc gagctccgat
    14221 attcacttcc accgaaaccg cgggcgcgcc cagggtgagg gcgttattga tcacgttgct
    14281 tcccgccgag ttcgttgcca gaatgctgcc attcagcgtc aggggattgc cccaaatcga
    14341 atagccgccc ccggcgccgg caaaccgtat ctggcttacc gaaagacccg gcaggttatt
    14401 cgtgttggtt agtttcaaag cgcccgcggg aaagatcaat atatcggcac tgccgggaac
    14461 tcccgcatag ccccagttgg cactctcgct ccaattgccc gtagtgccgc ctcccgacca
    14521 gctatatgtc gcccccgggc tatctgctgt caccagcagc acgctggccc acagcaccag
    14581 tacggccagc cgcgccagcc ggtgttcagg aatgaagtta tacctctgcg gttcgaaaga
    14641 cggtcgagcc ggtaattgct cagtcttttt catagcgtac ctttgttttc tgccaggcac
    14701 gattctaatc aaatcccccc gccgcataca agtataataa ataaaataat ttcagcccgc
    14761 tgttcccctt gatctgcggt tttagaaccc tgccgcttct atctaaaccc cgctcgttcc
    14821 cattgcggct aacgcgggat gtgctgcctt tccttagccg gaacgattca gatttcgctt
    14881 tccattcgac cccgcaaaac gtgcaagtcc ctggtttgaa gagcgaaagt cattcggaat
    14941 ccaatcgcca aaccgttgaa cccaccaacc aaacggttcg ctgagactat ccgctggcct
    15001 accaggtccg tttcgactcc ggtgaagcca gggcgcacga ttggactgca tcacgacaat
    15061 gtttcatacc acttctggca cgtaaatgca ctattctccg ctgcggtagc gtttact
//
LOCUS       DAOPQG010000086        15110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4669_length_15110_cov_14.144205, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000086 DAOPQG010000000
VERSION     DAOPQG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 15110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15110
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4669_length_15110_cov_14.144205"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1388
                     /locus_tag="PKI07_10100"
     CDS             144..1388
                     /locus_tag="PKI07_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC51442.1"
                     /translation="MNVRERFREVMNFNPSVRSLKWEFGYWGETINNWYAQGLPRQHY
                     PEVETRITTPTSTLYSYAWSNKGKGVSRLHNGLPVMAGGLYWPTQGFPLDFDVKAHFQ
                     MDYTQVMVDVNLLWDPMFEPVMVEEDAEQLTYVDIDGVKRTFLKETATIPTSVEWPIT
                     DRASWEKLKAERLNLKNISSRFPQNWPELVKQYKNRDYPLAVGGYPHGFFGTLSHLIG
                     YEYLFYWYGTEPDLVHDILKTFTDLWIAVYEEVLHQVEVDHWQIWEDISYGKGPMISL
                     PMVREFMCPYIKRVGDFLRAHGVQIILLDTDGDCNSLIQPFLEAGVTGMYPFETHCGM
                     DIVKVRKQYPRLQMLGGISKSEIQRGKERIDQLLQPVVEVLKTGGYIPFGDHFIPPDV
                     NFENFSYYRTQLNRIIEAAGNL"
     gene            1385..2860
                     /locus_tag="PKI07_10105"
     CDS             1385..2860
                     /locus_tag="PKI07_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOC51443.1"
                     /translation="MNAPGTIVETMAPVSRREIDNSQINGAFVWKVAFIAAMGGLLFG
                     YDFMVIGGATIFYEKYFNLGGRPDLLGLSVGSAPIGCILGAVFSMIFSDLYGRKKLLV
                     VASVLFGVSALGTAAAWNFLAFNIFRLVGGMGIGLAANLSPMYIAEISPTNYRGKVVT
                     TNQFTIMVGILLSQVVNLLIQQHGERIGALASGLTWNESFGWRWMFGAMAVPALGFFA
                     LIFTVPESPRWLVKKGCQQEAERVLAMVGGVEYARSEAADIASTISAEEVARINVREL
                     FEPKLLKIILLGMFLALVQQWSGLNSVFAYSHQIFKDAGIGVSGTMLSLVFQGLTMLL
                     FCIVAMFIVDHVGRKVLMLLGTLGISLIHLLIGLSFHFHRTGVPTVILVILAIAIYAM
                     TLAPIVWVLLSELFPNRVRGLALGISVVTLWVGYLILTYTFPIMRETIGTAKTFWIYS
                     AFLFAAFLVIWYALPETKGKSLEQIERDVFGMQVTQASERT"
     gene            2871..4118
                     /locus_tag="PKI07_10110"
     CDS             2871..4118
                     /locus_tag="PKI07_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51444.1"
                     /translation="MAKAQIHSTFEKGQGVLRLLPIFVPRLFGKAGRRLRLHPDDYYA
                     LGMKRGSIKERWFSSVIPANNGPLAAKDEGMSYVSIDVRDPKEKFLLRDAVEELGARL
                     IGRDLWSKYGTWPMYSKFFDYEGPLFHHLHLNDAAARRVGRIGKPGAYYFPPQLNNHL
                     GTFPHTYFGFAPETSRQEVRQRLLQYETADNRITELSRAYRIELGTGWYTPPGVVHAP
                     GSVLTYEPQWNSDVNSVYENVAAGELYPYEFLVENCPPGKKRDIDYGMSLMDWEKNID
                     PRYKQRFFRPPVTIRCDAEVVEKWIVYANDYVSAKELTLGPGRSATIKDPVAYGCIIV
                     QGHGKFGVYDAEAASMLRFGQASADEYFVSEDAAKQGVHIVNESLCEPMVILKHFGPN
                     HPAVPSCASHSKTNSRTIKRKKS"
     gene            4115..5059
                     /locus_tag="PKI07_10115"
     CDS             4115..5059
                     /locus_tag="PKI07_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC51445.1"
                     /translation="MKLQNYEKKNAVIREKFQALKKARKLGKARLNLSWSNWGFGLES
                     LADSAARLARANIRYIELHGNHYGADLGYHPRETLKILGHYNIKVAGICGMFSPDNDL
                     SSNRAIHRQNAIDYIRRELDFGSAVGAGYLLIVPGAVGRPVPYDQSEFERSVATLRIV
                     ASDLAKSKIKAAVEPIRSAEVSLIHTIADAKRYIAAVDHPAIKHINGDVYHMQVEESH
                     IGSALLDAGPMLTNLHMADSNRCALGDGALDLDTIIMALYVIGYNRPGCYVTPEPLGP
                     GGDPYPAMHGKPDVKSLDKMVITTAEYFRERESALLDS"
     gene            5136..6812
                     /locus_tag="PKI07_10120"
     CDS             5136..6812
                     /locus_tag="PKI07_10120"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOC51446.1"
                     /translation="MSAQYTIGLDYGTNSVRALIVKTADGQEVATAVWPYEHGNAGVI
                     LGRDPNLARQHPGDYIKGAEVTIRNALADAKKHVRGFKPEQIVGIGVDTTGSTPLPVD
                     ASGQPLAFQKRFHKNPAAMAWLWKDHTGVVEAAEITALAQKLRPHYLAKCGGTYSSEW
                     FFSKILHCLRTAPEVFNAAYTWVECADYIPATLTGTEHPEKLTVGICAAGHKAMYNDA
                     WGGYPDAKFLSRLDPKLGKLRARLRPQARAIDGSVGGLTACWAQRTGLRAGIPVAVGA
                     FDAHLGGVGSGIAPGTLVKIIGTSTCDMMVVPGDQPLADIPGLCGIVKGSILPGYYGL
                     EAGQSAVGDIFNWFVNYLQPGGKKAGTHEQLTREAARLKPGESGLLALDWNNGNRTIL
                     VDQCLTGLLLGQTLYTTPAEIYRALIEATAFGALTIINRFEEYGVRVQQVVNCGGIAE
                     KNPLVMQIYADVTGRPMKISRSAQTCALGAAIAGAVVAGTYKNYAAAQRAMTALKPRV
                     FKPSPEAHNTYRQLYGLYKTLHDAFGTRKWRGNLHPVMKQLIEIRSRVRQ"
     gene            6819..7508
                     /locus_tag="PKI07_10125"
     CDS             6819..7508
                     /locus_tag="PKI07_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOC51447.1"
                     /translation="MLEKLKRDVWQANLDLVARGLVVQTWGNASGIDRERGLIVIKPS
                     GVPYARMKPRHMVVVALGTGKVVEGTLKPSSDTPTHLTLYRAFREIGGVVHTHSLQAT
                     AWAQARRDLPCCGTTQADYWYGAVPCTRLLTAKEIRTDYETETGRIIVERFAKLNPLH
                     CPGVLVAGHGPFTWGQDVSAAVHNAVVLEFIAQLQTETLRINPGVAPLPPALIQKHFL
                     RKHGATAYYGQ"
     gene            7692..11537
                     /locus_tag="PKI07_10130"
     CDS             7692..11537
                     /locus_tag="PKI07_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOC51448.1"
                     /translation="MLLSSALAPTAGVQLVVFLPTNGNWNVAANWSSGEVPTPAQSPI
                     ILNGSVAVLDDNAGTCANLYVGQGAGPVFKGTLLVRKGASLSVAAMLLGRDFSNFGQV
                     DQMGGDLVVNGYLSIGDAAGGGSGASGEYNLSAGSLILPAADTFVAVGNQGVGRLLVC
                     GSAMLRTPSLFIGNGPGSSGSRMFQWGGAVHVGNFTIGTPATNKCAYTISSGVLQWGG
                     LLQVQDPLVLQGSRFWLQRTNSGGVGLQLTDTATLQFQLDAGGIAPVQMTGSQISIAT
                     GTKVVIDASVYSRWSAQPGAFTLIQHDGYAAQDQFTATNITFTGLVDLVPSLVCTTNS
                     VQLVLAAPTNGVSRAHQGLLFEYWELPINPGETPAISAPLSALPDFANGLVITHPVLG
                     KLVNDFDLSARLRDTNFFARFTGYLDVPTNGAYTFYLNSRHGSRLWIDGALVVNNDGA
                     HAVREVSATTNLTAGLHRIQAGYFHNTGTLTFQVKWAGPGWVKQAIPESALFLAARFD
                     QWVFKSSYHNITYDQDMIYNYAPSFIYDETEGLYKIWMCGTGGGGAVGGDHILYKEAT
                     SLEGLLRAPPRIALAPSLDPTKFDQVHACDPNVYRVGNLYYLTYSGNTDNTSLPAITR
                     IGMAVSTNGGRTFTRLHDGTHIIENPNTVADAYGDGQSAVVQANDGYYYMIYTDFPGG
                     TNAGCERVIRSPDPAFSPGSFTNLACLDPGLVGNSVDLLYDATNSQFIVVNGLRLSYF
                     DANWNHLRTCSYSNRFAWTLGEGHSLLGDSRKRPICYSPDNVPSLVFAAATTEFPTNT
                     ALWAPWVEGDLKYLIVPQSTAPSLARAQLISQGVAFAGDGTGIQDGAMLEVTNSFTVD
                     FWARPTLEANVVAEATSGIPGTWGQRYLAHPELGSLAWADGHAGMGVSVGRNCVQVFE
                     HSGNYLPALLSWQANLDDWTHIAVVYSNKTPSLYINGAFVHSGLTSTQLCVHPSARNF
                     GGSAYGYYAGQAWNYRVWNRPLASAEIHDLATAGVETNLASALIGEWLLDPPLSQATA
                     PGAKALALAVAGRVAGETYACQLLDNAAGRFTLNPDTGLITVLNADLLNFATNTAVSL
                     TARAIDSRSRNSDRQFSLMVLATNDPPFIAAISNRTLLAGGTFSLSIPASDPDVPPQP
                     LTFSLLSGPGGASLNATSGMFQWRPTLSQVGYQTQVRVKVRDNGIPNLSATQSFQLTV
                     AAPAPPVLRPFAVSNGQFHLVITGDSGPDYTVQAATNLANPIWVGLLTNLSATPPFVF
                     TDPLVPYFTQRYYRVLLGP"
     gene            11549..12604
                     /locus_tag="PKI07_10135"
     CDS             11549..12604
                     /locus_tag="PKI07_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51449.1"
                     /translation="MRTLISIWIGSLLLCGAAETPRYCDGRPAAKYRLDALDQGVVLR
                     HKGGPGQCDDFGARDIWVFQEGNTFYLHYDGAGPQGWLTCLATSKDLVHWEKKGPVLE
                     LGQPGEPDSASASYGVTFKAGKFWHMFYLGTPNVTPPPGRVPMFPYQTLKAKSRTPAG
                     PWIKQKSVIPFRCQPGTFYSVTASPGCVLKHRGEYLLFFSGATDNPVKRTIGLVRTKN
                     LDAAWTVDLQPVFSPEEQVENTSIYYEPKCRTWFLFTNHIGIDERGEYTDAVWVYWSK
                     DLTHWDARKKAVVLDRKNCTWSKDCIGLPSVIKCKNRLAVLYDAPGGTSIDHLYRDVG
                     LAWLNLPLVPPDQITHE"
     gene            12597..14021
                     /locus_tag="PKI07_10140"
     CDS             12597..14021
                     /locus_tag="PKI07_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose isomerase"
                     /protein_id="HOC51450.1"
                     /translation="MNKVKLGLFGIGLEAYWPQFKGLKPRLEGYLKVVADRLRHPGVD
                     VVNLGLIDSPPKALAAGHRFRQEDVDLIFLYVTTYALSSTVLPVVQRAKVPVIILNLS
                     PAPAIDYAQFNRMGDRTAMTGEWLAHCAACPVPEIANVFNRANIAFHQITGMLHEDPV
                     CWQEVGDWIEAARVANVMFHNRLGLMGHYYCGMLDIYSDLTLHCATFGGHMEIIEVDE
                     LAAMRREVTAKQIQRRVAEFNRVFDVQPDCSPAELARAARTSVALDRLVKEHDLGSMA
                     YYYMGRGNDENEDAISSIILGNSMLTARGIPVAGEYEVKNAQAMKILDSFGVGGSFTE
                     YYAMDFNDDLVLMGHDGPGHIAIAEGKTKVRPLRVYHGKVGRGLSVEMSVKHGPVTLL
                     SVVEAANGRLKLLVAEGESVPGPILEIGNTNSRYRFDIGARQFVNNWNLHGPAHHCAV
                     GVGHIASKIEKFGKLLGMEVARVC"
BASE COUNT         3015 a         4518 c         4293 g         3284 t
ORIGIN      
        1 gttttgctgc tcatgcggca gcattgtgtt acttatttat gcgagagtca ataggggaat
       61 agttgcagca ttccccggtt gagcgaagta ccatgaattg gggacaagcc tcgcgtaggc
      121 gcttcgctta cactgtcccc taaatgaacg taagagaacg cttccgcgaa gtgatgaact
      181 tcaaccccag cgtgcgctcg ctgaaatggg aattcggtta ctggggtgaa acgatcaaca
      241 actggtacgc gcaaggattg ccccgccagc actatccgga agtggaaacc aggattacca
      301 ctccgacttc gacgctgtac agctatgcct ggtcgaacaa gggcaagggg gtcagccggc
      361 tgcacaacgg gctgcctgtc atggccggcg ggctttattg gccgacccaa ggctttccgc
      421 tcgacttcga tgtcaaagcg catttccaga tggattacac ccaggtcatg gtggatgtga
      481 acctgctgtg ggatccgatg tttgagcccg ttatggtgga ggaggatgcg gaacagttga
      541 cttatgttga tattgacggc gttaaaagaa ctttcctgaa ggagacggcg accattccca
      601 cttctgtcga gtggccgatt acggatcggg cgagctggga gaaactcaag gctgaacggc
      661 tcaacctcaa gaatatctca agccgatttc cgcagaactg gccggagttg gtcaaacaat
      721 acaagaaccg ggattacccg ctagccgtgg gggggtaccc tcatgggttc ttcggaacgc
      781 tcagccatct gattggctat gaatacctct tctactggta tgggaccgag ccagatttgg
      841 tgcatgacat tctcaaaacc tttaccgatc tgtggattgc ggtttacgag gaggttctgc
      901 atcaggtgga ggtggatcac tggcaaattt gggaggacat ctcctatggc aaggggccga
      961 tgatctcgct gccgatggtc agagaattca tgtgccctta catcaagcgg gtgggggatt
     1021 tcctccgggc gcacggcgtg cagattatcc tgttggacac tgatggcgat tgcaactcgc
     1081 tcattcagcc cttcctcgaa gcgggggtta ccggcatgta tcccttcgaa acccactgcg
     1141 gcatggatat cgtcaaagtg cggaagcaat atccccggct gcaaatgctg ggcggcattt
     1201 ccaaatcgga aatccagaga ggcaaagaac ggattgacca gttgctgcag ccggtggtgg
     1261 aagtgcttaa gacgggaggc tatattccct ttggggacca ctttattccg ccagacgtga
     1321 atttcgaaaa cttcagttat tatcgcactc aattgaatcg cattattgag gccgccggca
     1381 atttatgaac gccccgggaa ccatcgttga aaccatggcc ccggtttcac gccgggagat
     1441 tgataactct cagatcaacg gggcctttgt ttggaaagta gcctttattg cggcgatggg
     1501 gggcctgctt ttcggctacg acttcatggt catcgggggt gccaccatct tctacgaaaa
     1561 atacttcaac ctcggggggc ggccggacct gctggggttg agcgtcggat ctgcgcccat
     1621 cggctgcatt cttggcgcgg tcttctcgat gatcttttcc gatctttacg gccggaaaaa
     1681 attgctggtg gtagcctccg tccttttcgg cgtttccgcg ttgggcaccg cggctgcctg
     1741 gaactttctg gcgttcaaca ttttccggct ggttggcggc atgggcatcg gcctggccgc
     1801 gaatctttcg cccatgtaca tcgccgaaat ttcccccacc aattaccggg gcaaagtggt
     1861 taccaccaac cagtttacca tcatggtcgg gattctgctt tcccaggtgg tcaaccttct
     1921 catccagcag cacggggaac ggatcggcgc gcttgcttcg ggcttaacgt ggaatgagtc
     1981 ctttggctgg cgctggatgt ttggcgcgat ggcggttccc gccctgggct tcttcgcgct
     2041 gatttttacc gtgcccgaaa gcccccgctg gctggtcaaa aaaggctgcc agcaagaggc
     2101 ggagcgggtg ctcgcgatgg tgggaggcgt tgagtatgcc cgaagcgaag ccgccgatat
     2161 cgcctctacg atttcggcag aggaagtggc gcgaatcaat gtgcgcgagt tgtttgaacc
     2221 aaagctcctg aaaatcatcc tcctcggcat gttcctggcc ctggtccagc aatggagcgg
     2281 gctcaatagt gtttttgcct attctcacca gatctttaaa gacgccggca tcggtgtctc
     2341 cggcaccatg ctttccctgg tcttccaggg gctgaccatg ctcctgtttt gcattgtggc
     2401 catgttcatc gttgaccatg taggtcgcaa agtcctcatg ctgcttggca cgctggggat
     2461 ctcgctgatc caccttctta ttgggcttag ttttcacttt catcgaaccg gggtgccgac
     2521 ggttatcctc gttatactgg ccattgctat ctatgccatg accctggccc caattgtttg
     2581 ggtgctcctt tccgaattat tccccaaccg cgtgcgcggg ttggcgttgg gaatctcggt
     2641 cgtaacgctt tgggtaggct acctgatcct cacctatacg ttccccatca tgcgcgaaac
     2701 tattggcacg gctaaaacct tctggatcta ctccgcattc ctttttgccg cctttttggt
     2761 catttggtat gcattgcctg aaaccaaggg taaatcgctt gaacagattg aacgtgatgt
     2821 attcggcatg caggtgaccc aggcatcgga gcgaacctga ccgtactatt atggccaaag
     2881 cacagataca ttcgacattt gaaaaagggc agggcgtctt gcgcttgctg ccgatctttg
     2941 ttccacgtct tttcggcaag gccggccggc ggttgcgcct ccatcctgat gattactacg
     3001 cgctcggcat gaagcggggc tcgattaaag aacggtggtt ctcatcggtc attccggcca
     3061 ataacggccc gctggctgca aaagatgaag gcatgagcta cgtgtccatt gacgtgcgcg
     3121 atccgaagga gaaattcctg ctgcgggacg ccgtcgaaga attaggggcc aggctaattg
     3181 ggcgggacct ctggtctaag tacgggactt ggcccatgta ttccaagttc tttgattacg
     3241 aggggccgct ctttcatcat cttcacctga atgatgccgc cgctcgccgg gtggggcgga
     3301 tcggcaagcc gggagcctat tactttccgc cgcaactcaa taaccacctc ggcactttcc
     3361 ctcataccta tttcggtttt gcgcctgaaa ccagccgcca ggaagtgcgc cagcgactgc
     3421 ttcaatacga gacggctgat aaccgtatca ccgaactttc ccgcgcatac cgcatcgagt
     3481 tgggcaccgg ttggtacact cctcccgggg tagtgcatgc gcccggatcg gtgttgacct
     3541 acgaaccgca atggaatagc gacgttaatt cggtttatga gaatgttgct gccggggaac
     3601 tgtatccgta cgagttcctg gtcgagaact gtccgcctgg caagaagcgg gacattgact
     3661 acgggatgag cttgatggat tgggaaaaga atattgatcc acgttacaag caacgttttt
     3721 tccggccgcc cgtaaccatt cgctgcgatg ctgaggttgt cgaaaaatgg atcgtttacg
     3781 cgaatgacta cgtttccgcg aaagagctga ccttgggacc gggccgatca gcaacaatca
     3841 aggatcccgt ggcctacggc tgcattatcg tccaaggcca cggcaaattt ggcgtctatg
     3901 atgccgaggc ggcttcgatg ctccggtttg ggcaggccag tgccgatgaa tacttcgtga
     3961 gcgaagatgc cgcgaagcaa ggagttcaca ttgtcaatga gagcctctgc gaacccatgg
     4021 tcattttgaa gcattttggc ccaaaccatc ccgccgtgcc atcgtgcgca agtcattcca
     4081 aaacaaatag cagaaccatc aaacgtaaaa aatcatgaaa ttgcagaact acgaaaagaa
     4141 gaacgcggtc atccgcgaga agttccaggc acttaagaaa gcccggaagc tgggcaaggc
     4201 acgattgaac ctctcctgga gcaactgggg ttttggcctt gagtcgctcg ctgattcggc
     4261 tgcgcgcctg gccagggcga acatccgcta tatcgagttg cacgggaacc actatggagc
     4321 ggacctcggc tatcatccca gagaaaccct gaaaatacta ggccattaca atatcaaagt
     4381 tgccgggatt tgtggaatgt tctccccgga caacgacctt tccagtaatc gggcgattca
     4441 ccgtcaaaat gcgatcgatt atattcggcg cgagttggat tttggttcgg ctgttggcgc
     4501 gggctatctg ttgatcgtgc cgggggcagt aggccgcccc gtgccgtatg accaatccga
     4561 atttgaacgt tcagtcgcaa cgctgcgcat tgttgccagc gatttggcaa aatcgaagat
     4621 caaggcggcc gtggaaccta tccgctcagc cgaggtgagc ctgatccaca cgatcgcaga
     4681 tgcgaagcgc tacatcgcgg cagtcgatca tccggccatc aagcatatca acggcgacgt
     4741 ttaccatatg caagtggaag aaagccacat tggcagtgct ctcctggacg cgggccccat
     4801 gttgaccaat ctgcacatgg ctgatagcaa ccgctgtgcg ctgggcgatg gggcgctcga
     4861 tctggatacc attataatgg cgctctatgt tattggctat aaccgccccg gctgttacgt
     4921 tacccccgag cctctggggc caggcggcga tccgtatccc gctatgcatg gcaaacccga
     4981 tgtaaagtcg ctcgacaaaa tggttattac caccgccgag tatttccggg agcgggaatc
     5041 agcgctgctt gattcatgag cagtgttatc ctgacagagc cttttcggac gaagggaagg
     5101 catgccgcta aggaaccccc aggcctattg agtctatgag tgcccaatac actatcggtt
     5161 tggattatgg aactaattcg gtgcgtgcgc tgatcgtcaa gaccgccgat ggccaggaag
     5221 tggccacggc cgtttggcct tatgagcacg gaaacgcagg cgttatcctt ggacgcgatc
     5281 ccaatctggc caggcaacac cccggggatt acatcaaagg ggcggaggtt accattagaa
     5341 acgccctggc cgacgcgaaa aagcatgtgc ggggcttcaa accagagcag attgtcggca
     5401 ttggtgtgga cactacgggc agcaccccgt tgccggtgga tgccagcggg cagccgctcg
     5461 ctttccagaa aaggttccac aagaaccctg ctgcgatggc ctggctctgg aaggatcata
     5521 ccggtgtagt tgaggccgct gaaattactg cgcttgccca aaagctacgc ccacactatc
     5581 tcgccaagtg tggcggcacc tattccagcg aatggttttt cagcaagatt ctgcattgtc
     5641 tgcgcaccgc ccccgaagtc tttaacgccg cgtacacgtg ggttgaatgc gccgactata
     5701 tcccggcgac gcttactggc acggagcatc ccgaaaagct gaccgtgggc atttgcgccg
     5761 ccggccacaa agcaatgtac aacgatgcgt ggggaggata tccggatgcc aaattcctca
     5821 gccggcttga cccgaagctg ggtaaactgc gggcgcggct gcggcctcag gcgcgcgcga
     5881 ttgacggctc cgtcggtggc ctcactgcgt gttgggccca gcgcacgggt ttgcgtgccg
     5941 gcatcccggt agccgtgggg gcctttgatg cccaccttgg cggggtcggc agcgggatcg
     6001 ctccgggcac gctcgtgaaa atcattggca ccagcacctg cgatatgatg gtggtgcccg
     6061 gggatcagcc gctcgcggac attcccggac tctgcggcat cgtaaaaggc agtatccttc
     6121 ccggttatta tggccttgag gctggacagt cggcggtggg agacatcttc aactggtttg
     6181 tcaattacct gcagccaggc ggcaaaaagg ctggcacgca cgagcaattg acccgggagg
     6241 cagcgcggct gaagccaggt gaatccgggc tcctggcctt ggattggaac aacggtaacc
     6301 gcacgatttt ggtggaccag tgcctcactg gattgctttt gggtcaaacg ctttacacca
     6361 ctccggccga aatctatcgg gcacttatcg aggccaccgc gtttggcgcc ttgaccatca
     6421 ttaatcgttt cgaggaatac ggcgtgcgcg tgcaacaagt ggtcaactgt ggcgggattg
     6481 cggagaagaa tcccctggtc atgcaaatct acgccgacgt tacgggccgc cccatgaaaa
     6541 tttcccgatc cgctcagacc tgcgctctgg gagcggcgat cgcgggcgca gtggtggccg
     6601 gcacctacaa gaactacgcg gcagcgcaac gggcaatgac cgcgctcaaa ccacgcgttt
     6661 tcaagcccag cccggaagcg cataatactt atcgccagtt atacgggctc tataaaaccc
     6721 tgcacgatgc tttcggcact cggaagtggc gcggcaatct gcatccggta atgaagcagc
     6781 tcatcgaaat ccgcagccgc gtccggcagt agtctgccat gctcgaaaag ctcaagcggg
     6841 atgtttggca ggccaacctc gatctcgtgg cccgggggtt ggtcgtgcaa acgtggggta
     6901 atgcgagcgg cattgatcgc gagcgcggat tgatcgttat taaaccttcc ggcgttcctt
     6961 atgcccggat gaagccgcgc catatggttg tggtcgcgct ggggaccggc aaggtcgtcg
     7021 agggaacgct taagccaagt tcggatacgc cgactcatct cacgctctac cgcgccttca
     7081 gggaaattgg cggagtggtt cacacgcata gtcttcaggc gaccgcctgg gcccaggcgc
     7141 ggagagatct gccgtgctgc ggcaccacgc aggcggatta ttggtatggt gcggttcctt
     7201 gcacccggct gctgacggcc aaggaaatta ggactgatta cgagacggaa acggggcgga
     7261 ttattgttga gcgattcgca aagctgaatc cgttgcattg ccccggggtg ctggtggccg
     7321 gccacggtcc gtttacatgg ggccaggacg tgtctgcagc ggttcataat gcggttgtgc
     7381 tggagtttat tgcccagttg caaacggaga ccctgcggat taatcccggg gtagcgccac
     7441 ttccaccggc gctgatccag aaacactttt tgcgcaaaca cggggccacc gcatactatg
     7501 gccagtaaag ttctgcccat gccagccgcc gctcacgggc ccgccataaa gcatgacgtg
     7561 acttgattgc gccccggttg aacgccatga aacccggctt ggagcatcct aaaagggatc
     7621 cgcaaggggt tccttcaggc ctccggcttc aaaagccgca tcgaagaagc ctcgccgggc
     7681 tttgcgcgtg cttgctgctt tcatcggccc ttgccccgac cgccggagtc caactggtgg
     7741 tttttctgcc gaccaacggg aattggaatg ttgccgccaa ctggagttcc ggcgaggtgc
     7801 caactcctgc ccaaagcccc attatcctca acgggagcgt cgcggtgttg gatgacaatg
     7861 ctggaacctg cgccaatcta tatgtggggc agggcgccgg cccggtcttc aaaggcacct
     7921 tgcttgttcg taagggggcc tcgctttccg tcgccgcaat gcttctcggc cgggatttct
     7981 ccaatttcgg gcaggttgat cagatgggtg gggacttggt cgtcaatggc tatctgagta
     8041 ttggtgacgc tgccggtggc ggttccggcg cgagtggcga gtacaacctt tccgccggca
     8101 gtcttattct tcccgcggct gatacttttg tggctgtggg caaccagggc gtgggccgct
     8161 tgctggtctg tggctcggcc atgctgcgca cgccgagcct gtttattggc aacgggcccg
     8221 gctcttccgg ttcccggatg ttccaatggg gcggcgccgt tcacgtcggt aattttacca
     8281 ttggcacccc cgcaacgaat aaatgcgctt acaccatctc atccggggtt ttgcaatggg
     8341 gcgggcttct gcaagtacaa gacccgctgg tcctgcaagg ctcgcggttt tggctgcagc
     8401 gcaccaattc cggcggcgtg ggtctgcaac tcacggacac cgccaccctc caattccaac
     8461 tggatgccgg cggcatcgcg ccggtccaaa tgaccggctc ccaaatctcc atcgccaccg
     8521 gcaccaaagt ggttattgat gcttcggtct attcacgctg gtccgcgcaa cccggcgcct
     8581 ttaccctcat ccagcatgat ggctatgcgg cccaggatca gtttacggcc acgaatatca
     8641 ctttcacggg tttggttgat ctggtgccct cgttggtctg cactaccaat tcagtccagc
     8701 ttgttctggc tgcccccacg aacggcgtgt cccgcgccca tcagggattg ctcttcgagt
     8761 attgggaact gccgatcaac ccgggggaaa cgccagccat ttccgcacct ctctccgccc
     8821 tgccggactt cgccaacggc ttggtgatta ctcacccggt gctgggcaaa ctcgtcaacg
     8881 atttcgacct ctccgcccgg ttgcgcgaca ccaacttctt tgcccggttt acaggttatc
     8941 tggacgttcc gaccaacggc gcatatacct tctacctgaa ttcccggcat ggttcgcggc
     9001 tttggattga tggagccctg gtcgtgaaca acgatggcgc gcatgccgta agggaagttt
     9061 cggcgaccac aaatcttacc gccggcttgc accggattca ggccggctat ttccataata
     9121 ccggcaccct tactttccag gttaagtggg ccggacccgg gtgggttaaa caggcgattc
     9181 cggagagcgc gctattcctg gccgcgcgct tcgatcaatg ggtcttcaaa tcatcctatc
     9241 acaatatcac gtacgatcag gatatgattt acaactatgc cccctcgttt atctacgacg
     9301 agacggaggg gctctacaaa atctggatgt gtggcactgg cggaggaggc gccgtgggag
     9361 gcgaccacat tctttacaag gaagccacgt ctctcgaagg cctgttgcgg gcgccgccgc
     9421 ggatcgcgct ggctcccagc ctggatccca ccaagttcga ccaggtccac gcctgcgatc
     9481 cgaatgttta tcgcgttggc aacctttact accttaccta cagcggcaac acggataaca
     9541 ccagcttgcc ggccatcacc cgcatcggca tggccgtttc caccaacggc gggcgcacgt
     9601 tcacacggct ccatgacgga acccacatta ttgaaaaccc caacacggtt gcggatgcct
     9661 atggcgatgg ccagtcggcg gtggtccagg cgaatgacgg ctattactac atgatctata
     9721 cggactttcc cggcggcacg aatgccggct gcgaacgcgt catccgctcc ccggatcccg
     9781 cattctcccc gggcagtttc accaacctcg cttgtctcga cccggggctg gtggggaaca
     9841 gtgtggacct cctttacgat gccaccaact cgcaattcat tgtcgtaaac ggcctgcgcc
     9901 tgagctactt tgacgcaaac tggaatcacc tccgcacctg ctcctattcc aaccgcttcg
     9961 catggacatt gggcgagggg cattccctgc tgggagactc gcggaaacgc ccgatctgct
    10021 atagcccgga caacgtgcct tccctggtct tcgccgcggc aacgaccgag tttcccacca
    10081 acaccgctct ttgggcgcca tgggtggaag gcgacctcaa atatctcatc gttccgcaaa
    10141 gcacggctcc ctcccttgcc cgcgcccagt tgatctcgca gggcgtggcc ttcgcggggg
    10201 acggcacggg cattcaggac ggggcgatgc tggaggtgac caacagcttt acggttgact
    10261 tctgggcccg gccgacactc gaggccaatg ttgttgcgga agccacctcc ggcatacccg
    10321 gcacctgggg gcaacgttac ctcgcgcatc cggagttggg ctctctggcg tgggcggacg
    10381 gccatgccgg catgggggtg tctgtggggc gcaattgcgt tcaggttttc gaacactccg
    10441 gcaactactt gcccgcgttg ctttcctggc aggccaacct ggacgactgg acacacattg
    10501 cggtggtcta ttccaacaaa acgccgtcgc tgtatatcaa tggcgctttc gtgcatagcg
    10561 gcctcacgag cacgcagctc tgcgtgcatc cttccgcccg caacttcggc ggcagcgcct
    10621 atggctacta cgccgggcag gcgtggaatt atcgcgtctg gaaccgcccc ctggcctctg
    10681 ccgaaatcca cgacctggca accgcgggcg tcgaaaccaa tctagcgtcc gccctgattg
    10741 gcgaatggct cctggatccc ccgctgagcc aggccacggc gccgggagca aaggccctgg
    10801 ctttggcggt ggccgggagg gtcgccggcg aaacctatgc ctgccagttg ctggataatg
    10861 ccgccgggcg attcaccttg aatcctgaca ccgggcttat aacggtgctc aacgcggacc
    10921 tgttgaattt tgccaccaac accgccgtat ccctcaccgc gcgagccata gactcccggt
    10981 cccgcaattc cgatcggcag ttctcgctta tggttctggc caccaatgat cctccgttca
    11041 tcgccgcaat ctcaaatcgc accctccttg cggggggaac cttttcgctc tccattccgg
    11101 cctcggatcc cgatgtgcct ccccagcccc tgacatttag tttgctcagc ggccccgggg
    11161 gcgcttcgct gaatgccacc agcggaatgt ttcagtggcg tcccaccctt tcgcaggtgg
    11221 gctaccaaac gcaggtccgc gtgaaggtgc gggacaacgg gattccaaac cttagtgcca
    11281 cccagagctt tcagttgacg gtggcagcac cggctccgcc ggttttgcgg ccgttcgcgg
    11341 tcagcaacgg ccagttccac ctggtgatca cgggcgatag cggcccggac tacaccgttc
    11401 aggccgccac caacttagcc aacccgattt gggttggact gctcacgaac ctgtcggcaa
    11461 cgccgccctt cgtatttaca gaccctttgg tgccgtattt tacgcagcgc tactaccggg
    11521 tgttgctcgg cccctaagtt ctcattttat gcgtactcta atctctatct ggatcggctc
    11581 ccttctcctc tgcggagccg cggaaacccc acgctattgc gacggccggc ccgccgcgaa
    11641 ataccgcctc gatgcgctcg accagggggt ggtgctccgg cacaaaggcg ggccggggca
    11701 gtgcgacgat ttcggcgccc gcgatatttg ggtattccag gagggaaaca ctttctacct
    11761 ccattacgac ggcgccgggc cacaaggctg gctgacctgt ctcgccacca gcaaagacct
    11821 cgtccactgg gagaaaaaag ggccggtcct ggaacttggc cagcccggtg agcccgactc
    11881 cgccagcgcg tcttatgggg tgaccttcaa agcgggcaaa ttctggcaca tgttctacct
    11941 cggcaccccc aacgtgaccc cgccgccagg gcgcgtgccc atgtttccct accagactct
    12001 caaggcgaaa agccggaccc cggccgggcc ttggattaaa cagaaatcgg tcatcccttt
    12061 ccggtgccag cccggcacat tttattcggt aacggccagc ccgggctgcg tgcttaaaca
    12121 ccgcggcgag tatctcctgt ttttcagcgg cgccacggac aacccggtta aacggacgat
    12181 tggcctggtg cgcacgaaaa accttgatgc cgcctggacc gttgatctcc agcccgtctt
    12241 ctcgccggaa gagcaagttg aaaacacctc gatctactac gaacccaaat gccgcacctg
    12301 gtttcttttc accaaccaca tcggcattga cgaacgcggc gaatatacgg atgcggtctg
    12361 ggtctattgg agcaaggacc tgacccactg ggacgcgcgc aaaaaagcgg tcgtgctcga
    12421 tagaaaaaac tgcacttgga gcaaggactg cattggcctg ccgtccgtca ttaaatgcaa
    12481 gaaccgattg gcggttctct atgacgcccc cggcggaacg agcattgacc acctctaccg
    12541 cgatgtcggc ctggcctggc tcaacttgcc cctggtgcct cctgatcaaa tcacccatga
    12601 ataaagtaaa gctcggactg tttggaatcg gcctggaagc ctattggcct caattcaaag
    12661 gactcaagcc gcgccttgaa ggttacctga aagtcgtcgc tgaccgcctc cgccacccgg
    12721 gcgttgacgt cgtcaacctc ggcctcattg attccccgcc caaagcactg gccgccgggc
    12781 atcgctttcg gcaggaagac gtggacctca tcttcctcta cgttacgacc tacgccctct
    12841 cctcaacggt tttgcccgtg gtgcagcgcg ccaaggtgcc ggtcatcatc ctcaacctct
    12901 cgcccgcccc cgccattgac tatgcccaat tcaaccgcat gggcgatcgc accgccatga
    12961 cgggcgagtg gctggcccac tgtgccgcct gccccgttcc ggaaatcgcc aatgttttca
    13021 accgggccaa catcgccttc catcaaatca ccggcatgct gcacgaggac cccgtttgct
    13081 ggcaggaggt gggagactgg attgaggccg cccgcgtggc caatgtcatg ttccacaacc
    13141 gccttggcct catggggcac tattactgcg gcatgctcga catctactcc gacctcacgc
    13201 tgcactgcgc gacgttcggc ggccacatgg agattattga agtggatgaa ctggccgcca
    13261 tgcgccggga ggtgacggcc aaacaaatcc agcgccgggt ggccgaattc aaccgcgtct
    13321 ttgatgtcca gcccgattgc tcccccgccg agttggcgcg tgccgcccgc acctccgtgg
    13381 cgttggaccg cctcgtcaag gaacatgacc tcgggtccat ggcctattac tacatgggcc
    13441 ggggcaacga cgagaacgaa gacgccatca gctcgattat cctgggcaat tccatgctga
    13501 ccgcccgggg aatcccggtt gccggtgaat acgaagtcaa gaacgcccag gccatgaaga
    13561 ttctcgacag cttcggcgtc ggcggctctt ttacggagta ttacgccatg gacttcaatg
    13621 atgatctggt cctcatgggc cacgacggcc cggggcatat cgccatcgcc gagggcaaaa
    13681 ccaaagtgcg cccactgcgc gtttatcatg gcaaggtggg acgcggcctt tccgtcgaaa
    13741 tgtccgttaa acatggcccc gtgaccctcc tttccgtagt cgaggcggcc aacgggcgcc
    13801 tcaaactcct ggtggcggaa ggcgaatccg tgcccgggcc aatcctggaa atcggcaaca
    13861 caaacagccg ttaccggttc gatatcgggg cccgccagtt tgtcaacaac tggaacctcc
    13921 acggccccgc gcaccattgc gccgtcgggg tggggcatat tgcctcgaaa atcgagaagt
    13981 tcggcaaatt gctggggatg gaagtggcgc gggtttgtta gcccctgagc gtgcagatgt
    14041 tcactgcttc cgccgaaaaa gcctgaaacc catcgcgccc cggccaaagc cgccctgcgg
    14101 ttgagctggg caaggcagcc tttgggcttc catagtgccg cgatgagcat cgctattgcg
    14161 gccgggtatt atctgtcgcg gccataaata atccgccctt gaatgactgg ctggcgtact
    14221 cgagcctcgt cgctcactgt ttggcgggcg gtaactggcc gtcctcggaa tccagaaagc
    14281 cgggttaacg acctgcatct gacgacctcc aaaccgttac cctatcgtgg ccctgccgtt
    14341 gcatgaccgc ctcatgcccc gtcatcccct tcctgcctcc tcaatttatg gcctgcaaag
    14401 cattccctgc ctgccgatat aatcgctgaa tgccgctggt catcctatcc atcatcgggt
    14461 tgtaagagga cgttcatcgc cagcaggagc accgccgcct cctcagccca acctgccaat
    14521 ggccttccgc cgttgctcgg tcccctcgcc gcagcttgag gtggccctcg cgtggcagcc
    14581 cgctgaggtt gcaagttcgt gcccagagcg gtttcttttg ctctccagtc cacttgtctg
    14641 cctgatcagt cgctccggtt ccggttagct ggacggccct tccgtcgccc ggctcagttt
    14701 cactggagcc gagcagatgc cagctcttca tcccatacac tactcggggg cgcgaggaga
    14761 gagagaagaa tccctgcgcc tcccggggtc ttttgttgtc ggcgcccggg tttcggtagc
    14821 tgaagggaaa aaggtttttc caaccccaaa aacgctcttc gggtttcggg ggcgggggtg
    14881 aaagcgtcta tcgctccaaa gcggccggcc gaccttgcgg acaacgcaag caatcacctt
    14941 tcggcagcct gcgcccagga ggggaactgt ccccgccagc gttggctccg gcccgtcgaa
    15001 aaaccagggc acgcgacaac acccgtccgc ccgtcccggg gttgccgaac gttcgcgtcc
    15061 tctccctccc gccttggaac ctcgttttac atcatccgcg actcgtccat
//
LOCUS       DAOPQG010000087        14739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4794_length_14739_cov_15.709207, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000087 DAOPQG010000000
VERSION     DAOPQG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14739
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_14739_cov_15.709207"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            561..866
                     /locus_tag="PKI07_10145"
     CDS             561..866
                     /locus_tag="PKI07_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51451.1"
                     /translation="MAITIWFYTTWLYFIFPDSLYAFRKLPIMFLEHRGRILAVRRFG
                     DAGFFQGAPKARPWRAVDNILNPRISGFARLMQTAAAALSIIGFARKFRFPRIIALW"
     gene            934..1491
                     /locus_tag="PKI07_10150"
     CDS             934..1491
                     /locus_tag="PKI07_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOC51452.1"
                     /translation="MPNTSRRLDRAHAGLVVVDIQERLLPAIYQPERVRENALRLVKG
                     AAVLRVPVLVTEQYRKGLGPTVPELAAAIPGFAPMEKLSFSACGADGFTRRLKELKIS
                     EVLLCGIEAHVCVAQTGLDLLDEGFRVFAAADAISSRTPENYGAGVDRMRAAGAVIVS
                     TEMALFELLGRAGTAEFKQLLPLVK"
     gene            complement(1498..2136)
                     /locus_tag="PKI07_10155"
     CDS             complement(1498..2136)
                     /locus_tag="PKI07_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC51453.1"
                     /translation="MKRTLLFAFASAAWLGAAATLAANELEPGFTALTDGKTFNGWRM
                     SEENQNTWTIQDGAFVAKGNRCHLFYEGDGKPFKDFHLKLEVMTGPQSNGGIYFHTQY
                     QATDWPRAGFECQVNNTHGDWIKTGSLWGLVNIGVSLVKDNQWWTQEIIVQGNKVTVL
                     LDGRKLLEYNEPAGAQPGKDFARKLSRGTFALQGHDPNSVVRYKNIRVKRLD"
     gene            2340..3371
                     /locus_tag="PKI07_10160"
     CDS             2340..3371
                     /locus_tag="PKI07_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC51454.1"
                     /translation="MANHCFHPSIEGAQHGAKSLTQFLDYAKKSGAAGAQPSNFMLQS
                     DKGFKSAKEIKNAFAKAKLKLDGVSAHCPFWVHTTAWTGSPTIRPFIPGDVARQSPEG
                     IEKWAEDYILRLLDLCAELGVKIVPMFWGVAFGWELATGYPWGFWKGGDYDLLKEGQE
                     RFVKKTAKIRKHARSLGMYLAHEIHPGTGAMCADDFNLLVEICAGDPCLAVNADPSHC
                     WEGESWETRFLKVASRIYACHVKNYVIRPGLPLRFMNGDWQKRAMQFVDIPTGDLNMM
                     RYVELLIHAGYPQRYCQVMGARTAPLIVEAESAYRAVDATSTNGIQYVRDHLCFPLAA
                     GSFEDGMGA"
     gene            complement(3481..3571)
                     /locus_tag="PKI07_10165"
     tRNA            complement(3481..3571)
                     /locus_tag="PKI07_10165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3535..3537),aa:Ser,seq:gga)
     gene            3714..5978
                     /gene="ilvD"
                     /locus_tag="PKI07_10170"
     CDS             3714..5978
                     /gene="ilvD"
                     /locus_tag="PKI07_10170"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOC51455.1"
                     /translation="MRSDTIKKGFERAPHRSLLRATGAIESEADWDKPFIAISNSYID
                     CVPGHVHLNEVGLLIKRLVRAAGGVPFIFNTIGIDDGIAMGHGGMKYSLPSRELIADC
                     VESMVRAHCFDGMICIPNCDKIVPGMLMGAMRLNIPTIFVSGGPMVAGIDQQAADDPL
                     PPHLRPATQSSDLVSVFKGVGELQAGKISRQDLQHLEQSACPTCGSCSGMFTANSMNC
                     LSEALGMALPGNGTILAVSKEREALYERAARAILKLVEADLKPRDIATLEAFDNAIAL
                     DVAMGGSTNTILHTLAIAREAGIAYDIKRIDDISRRVPCLCKVSPSSNYHVQDVHRAG
                     GIHTILGELKRMGALTLSCKTVTGKTLGENIDEWDVRSEKCVRWAKTARVSGCSAIVV
                     DPADKLGHAARTVSGNLAPKPLLFFPGDPRGITLWRFAAGFNAGDAAAIAALFSAEGE
                     LQVDETTTWKGPAAIEAGLKQALAGAKGLLRAELGQLDGTPLLIIWKYGKGAAKQAHC
                     LLRAPGLQDWQIQRLEHDYRPEAIKAAQPSGLMPYDPAFLFNARDCIRTRETAYTADG
                     GLAILYGGIAPEGCVVKTAGISDEFKQYCGPNFVFEGPCVIFESQEDACAGILAGKVK
                     AGDVVVIRNEGPRGGPGMQEMLSPTSYIVGMGLGDKVALITDGRFSGGTSGACIGHIS
                     PEAAEGGPIGLLREGDRLRIDFPNRRLDVVLPEAELAQRKKTWQPVKRDLSGWLARYQ
                     KLVSNASQGGILTS"
     gene            6879..7904
                     /locus_tag="PKI07_10175"
     CDS             6879..7904
                     /locus_tag="PKI07_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOC51456.1"
                     /translation="MFAQLKSLFSNDIGIDLGTANSVVYVRDQGIVLREPSVVAIQAG
                     TTNVLAVGEEAKRMLGRTPGNIVAIRPMKDGVIADFEITEAMLRHFIQKVHHRKLIAP
                     RVVVAVPSGITEVEKRAVKDSATHAGAREVYLIEQPMASAIGVGLPVHEPAGNMIVDI
                     GGGTCEIAIISLAGIVFSRSLRVGGDEFDETIVAHIKRAYNLMIGERTAEEIKMRIGS
                     AFPLEQELTMEVKGRDLSAGLPKTLTIRSEEIREALQEPLSSILESVRITLERCPPEL
                     SADLVDRGIVMAGGGSLLRGIDRLVAEETGLPVHKADDPLSAVAEGTGRVLQELHFLK
                     RVSAGSN"
     gene            8009..8812
                     /gene="mreC"
                     /locus_tag="PKI07_10180"
     CDS             8009..8812
                     /gene="mreC"
                     /locus_tag="PKI07_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOC51457.1"
                     /translation="MLKRSHYITLGLVVLLALVVLNLPGQTAARLKLALGSLFLPLFG
                     LAGSTQQVVGAAADAILPRRELVKQNAQLRQEEQRLRLQALQAAEIAGENARLRQLVG
                     WRAQTPWKLKLAKVVLREPANWWRSIQIDLGSRDGLQVNLPVLTTEGLVGRVASVSLT
                     RSQVVLLGDPNCKVAARVENETRDTGVISADGPLAAQLVRMDYLSRGAELKPAQRVFT
                     SGEGGIFPKGIPIGQIVDVQPVDYGLRYEARVRLAANLNALEEVWVMFP"
     gene            8809..9429
                     /gene="mreD"
                     /locus_tag="PKI07_10185"
     CDS             8809..9429
                     /gene="mreD"
                     /locus_tag="PKI07_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOC51458.1"
                     /translation="MNWLNTAFILGAAFLAVFGQAAFGSARFLAGAQVDLLPPLMVYA
                     ALSSGLTTVTLLAVCAGLWFDSLSANPLGVTVLPLFAVGWVIFLKRELILRDQPFAQA
                     VLGAAASVVAPALTLLLLLTMGSHPLFGWGTLWQLVVLCAGGALAAPVWFELFGWLQH
                     ALVHRGATESSFRPDREIRRGRRGALIGGYRGEVIDRARSKPRRPR"
     gene            9476..11497
                     /locus_tag="PKI07_10190"
     CDS             9476..11497
                     /locus_tag="PKI07_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOC51459.1"
                     /translation="MFIFDQLRTDDAHLRAVAVTVLAGLGILLGGLWWVQVVSADEYR
                     TRLERQSFRTVRIPAVRGQILDRNGRALAESRPTYNISLYCEELHDTFDAVYAEKATR
                     ARATLKLALQERESQLHRKLTKAERRPFLFTAAHRNALRREARCEVASNVVAQVSQRL
                     RQPLSLAVTNFEQHYQKRLALPYPLLTDLTPAQIARFEEQSTSPLGVDLEILPTRCYP
                     YGTTATHALGHLLRDDSSVEGEEAFFSYRLPDYRGVVGIEYGFDKELRGVAGAKSVLV
                     NNVGYRQTENIWSPAAPGRNVVLTLDLAVQQAAERALQGVAGSATRGAVVVMDVWTGD
                     LLALVSSPTLDPNWYVRGITHEQWRRITELNAEKNRATFENYMPGSVFKTVVGMAALE
                     SGLNPEEIIHVEPNPAQPAKGHKRVGKRTFKDLAPPGDYNFRRALKLSSNSYFITCGL
                     RAGPERIIRLGQRLHFGERSGLPTRQDAPGQFPSLQRLRTDWSDGNTGNLCIGQDPVW
                     VTPLQIAVLTAAIANDGIVLWPRLVDRIESVDPASPQPPQVFPRGRVRDHLGVSTRTL
                     RILRDAMLANVEDADATGRGAAVPGIRIGGKTGTAQVQDARNVKIGQTTWFASFAPYE
                     QPRYAVVVMVENGVSGGTTCAPIAGKIYSALLDRERAQLASSPALARNP"
     gene            11520..12725
                     /gene="rodA"
                     /locus_tag="PKI07_10195"
     CDS             11520..12725
                     /gene="rodA"
                     /locus_tag="PKI07_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOC51460.1"
                     /translation="MFDQDLQRHESRIDRLQIAALCGLMLLGTVFVYSATMVGESAAT
                     LVWYKQSWFRQAVWYGLGMGAATAVCCVDYHILARWAMVIYWSAILMLVLVLFFGTVR
                     FGARRWFDLGFFSLQPSEFAKLAFILAQANYLSRPVEELRGLATFWKSLGLMLLPFLL
                     ILKEPDLGSALVFLPTGLAILFVAGIPRRYLLRLLGGAGLLGALFVGNVLFAPPNWQI
                     KLEDYQRRRLLVYFGREEAPPPGASRAELARLRQQQADYSHNVRQALISVGSGGLIGK
                     GWRQGTQNALGFLPRPVAHNDFIFSVIAEEKGFVGSVIVLTLYGVVLFSGIRIAGQAR
                     DRLGKMMAVGVVALIFSHVFINIGMNIRLMPVTGVPLPLLSYGGSSVLGSLIAMGILQ
                     NVYFYRRTY"
     gene            12728..14689
                     /locus_tag="PKI07_10200"
     CDS             12728..14689
                     /locus_tag="PKI07_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOC51461.1"
                     /translation="MSDKNFSRRRRGMRFRPSRGIGHGQPQTPDRVGNQARAEAVAPP
                     PMEKIYDRRHDHEIDRAENIAAGLPPEGPRKSPAAPDAPGQSPESTPAPPPRGEQEKP
                     FTPVPIKEPSRGIVETLRSAANNLVKKVQRLIKPVKRVHKEVVINAETLETRVAVSEE
                     GRLEEFTIERTTEERLVGSIFKGKVRNLEDGLKAAFVDIGFEKNAFLHYWDIVPSNFD
                     SGVEIVDRGPRRRDKPKITQKDIPRLYPPGSEIIAQVTKGPIGTKGPRVTTNLVLPGR
                     YLVLLPNSDQSGISRKIENAQERQRLKKILRELAIPDGMGVIMRTVGEGQQKRYFVRD
                     LALLLEEWRQVQERIQKQPLASCVFQEPDLIERTVRDFLTEDVERIVVDSPKAHDRMR
                     EMIAKISKRSADKVKLYTDPQPIFDRFNITRQLENAFSRQVHLKSGGYIVVDETEALV
                     AIDVNSGRHKGGKDQEAAILKVNLEAADEIARQLRLRNIGGLIVLDFIDMKSRRDQQQ
                     VYQRMKEALHRDKAKTHVLPISQLGLMEMTRQRHSESVNAAVYDDCPYCKGRGKVKSA
                     LTMSVEIQRKLSEILKKRPRDESDFQLRIAVHPTILERLRTEDEKHLIEMEKRYFGKL
                     SFRADPGLHAEQFKIVNVANNEELGNASK"
BASE COUNT         2705 a         4676 c         4687 g         2671 t
ORIGIN      
        1 gggaaggctg gcgggcggga ggtctgcgcg caagggcaat tttttggggt tggcgttggc
       61 aggccggtgc ggagcgccgc gtgagggcac gcggcctaca ggggacggga gggtgcgggg
      121 ggcccgcggt tgccggaagt gggaagttgc aaaaggtcat gagatctgcc ggggtgtgct
      181 tccatttcct gtaggccgcg tgccctcacg cggcgctcgg gagccgttcg cccggcgctg
      241 agaaaagcac ctgcgcgcct gcccggattg tctgcgggaa gggctgccgg tcttggaggg
      301 caagctcatc accgccgatc ctctgcacgg ccagcacaaa ctggccccgg cgatcgggca
      361 aaatggcggc gaccatcccc ttccgggcaa agttaggcag ccctttctat ccccaactgt
      421 ccgagccctt ggggaacagc ttcgtgagag cctcaaaaac ggctcaatcc ggctcgaaaa
      481 acatggggca gtccagagcc cctgtaaatc aagtgcctta ccctcgattt gaggctgttt
      541 tcatctccca aaccggctct atggccatca ctatctggtt ttatacaacc tggttatatt
      601 tcatctttcc cgacagcttg tatgcgttcc gcaagctgcc catcatgttt ctcgagcacc
      661 gtgggcgcat cctggcagta cgtcgctttg gggatgccgg gtttttccag ggtgcgccca
      721 aggcgcgtcc atggcgcgcg gtggataata tcctgaatcc tcgaatttcg ggctttgcga
      781 ggctgatgca aactgccgct gcggccctct cgatcattgg ctttgcccga aagttcagat
      841 ttccccggat catcgcgttg tggtagtatc cggaactgtt cccgccgccg ggaaagccgg
      901 gcggcgggcc ggaagcattg agtcttgatt gttatgccga acacatctcg ccgtttggac
      961 cgtgcgcatg ccggcctggt tgtggtggac atccaggagc ggctgctgcc ggctatttac
     1021 cagccggagc gggtgcgcga aaacgcgttg cgcctggtca agggcgcggc ggtgctgcgg
     1081 gtgccggttt tggtgaccga gcagtatcgc aaagggctgg ggccgacggt gccggagctt
     1141 gccgcggcca ttccggggtt tgcgccgatg gaaaagctgt ctttcagcgc gtgcggcgcg
     1201 gacgggttca cccgccggct caaggagctg aaaatctccg aggtcctgct ttgcggcatc
     1261 gaggcccatg tctgcgtcgc gcagaccggc ctggaccttc tggacgaggg ctttcgggtt
     1321 ttcgcggccg ccgatgccat ctcgtcccga acgccggaga attacggggc cggggtggat
     1381 cggatgcgcg ccgcgggggc ggtcatcgtg tccacggaga tggcgctgtt cgagctgctg
     1441 ggccgggcgg gcaccgccga gttcaagcag cttctgccgc tggtcaagta ggccggctca
     1501 gtcgagccgc ttgacgcgga tgttcttgta gcgaacgacg ctgttggggt cgtggccctg
     1561 gagggcgaag gtgccgcggc tgagtttgcg cgcgaaatcc ttgccgggct gggcgccggc
     1621 gggctcgttg tattccagca acttccggcc atccagcagc acggtcacct tgttgccctg
     1681 gacaatgatt tcctgcgtcc accattggtt atccttgacc agggagacgc cgatgttgac
     1741 gaggccccag aggctgccgg tcttgatcca atcgccgtgg gtgttgttga cctggcattc
     1801 aaaccccgcg cgcggccagt cggttgcctg gtattgggtg tggaagtaaa tgccgccgtt
     1861 cgactggggg ccggtcatca cctccagctt gagatggaaa tccttgaacg gcttgccgtc
     1921 gccttcgtag aagaggtggc accggttgcc tttcgccaca aaggcgccgt cctgaatggt
     1981 ccaggtgttt tgattttcct ccgacatccg ccagccgttg aaggttttgc cgtcggtgag
     2041 cgccgtaaag ccgggctcca gctcgttggc ggccagggtg gcggccgcgc ccagccaggc
     2101 ggccgatgcg aaggcgaaaa gaagcgttcg tttcatggga gaccacacta aatcgccgcg
     2161 cccgcggcgc aagcaaaaag gagcgccacg gggaggcgaa aaccggccgg cgaggcgagc
     2221 cgcggcgatc ggaaggcgcg gcgcgcgcgg cgaaagaatg gggttgcctc cgccggcgcg
     2281 agggggtaat tgtctgggtg gtcggcaatg aataaacctg tttaacttct gatactacta
     2341 tggcaaacca ttgttttcat cccagcatcg aaggcgcgca gcacggcgcg aaaagtctga
     2401 cgcaatttct ggattatgcg aaaaagtccg gggcggcggg ggcgcagcca tccaacttca
     2461 tgctgcagag cgataaaggc ttcaagagcg ccaaagagat caagaatgcc tttgccaagg
     2521 ccaaactgaa actggatggc gtttcggcgc actgcccgtt ctgggtgcat acgacggcgt
     2581 ggacgggcag tccgaccatt cgcccgttca ttcccggcga cgtggccagg caatcgcccg
     2641 agggcatcga gaaatgggcg gaggattata ttctgcggct gctggatctg tgcgcggagc
     2701 tgggagtgaa gattgtgccc atgttctggg gcgtggcgtt tggctgggaa ctggccaccg
     2761 gctacccgtg ggggttctgg aagggcggcg attacgacct gctcaaggaa ggccaggagc
     2821 ggttcgtcaa gaagacggcc aaaatccgca agcatgcccg gtccctgggc atgtatctgg
     2881 cgcatgagat tcatcccggc acgggcgcca tgtgcgcgga tgatttcaac ctgctggtcg
     2941 agatttgcgc cggcgacccc tgcctggcgg tcaacgccga cccgtcgcat tgctgggagg
     3001 gcgaaagctg ggagacgcgg tttttgaaag tggcctcccg catctatgcc tgccacgtga
     3061 agaactatgt catccggccg ggcctgccgc tgcggttcat gaacggcgac tggcagaagc
     3121 gcgcgatgca gtttgtggac attcccaccg gcgacctgaa catgatgcgc tacgtcgagc
     3181 tgctgattca cgccggctac ccgcagcggt attgccaggt gatgggcgcc cgcacggcgc
     3241 cgctgatcgt tgaggccgag agcgcctacc gggcggtgga tgccacgagc accaacggca
     3301 tccaatacgt gcgcgatcat ctgtgcttcc cgctggccgc gggctccttc gaggacggca
     3361 tgggcgcgta agcggcggcc gggcttcggg gaattcacgg cagcggcccg gcctccggcc
     3421 ggggacggtc ccgcgcggcg cgcggattcc cgttgcgggg ttggggggga tttgggtgtt
     3481 tggcggagag actgggattc gaacccaggg tggggtataa gcccactcac gctctccagg
     3541 cgtgcgcttt aaaccactca gccatctctc cggccaattc gagcctactg tgcggcagcg
     3601 cggcccggtg cgcaatgttt ttcctcggct tcccggcgcg tgacaaagcg gccgcaagct
     3661 gctatcttcc cgcgtgactt ttcggcgatg gtcgccgaga atcaggtaat attatgcgct
     3721 ctgacaccat taagaaaggc tttgaacgcg ctccgcatcg ctcgctgctg cgggccaccg
     3781 gcgccattga atccgaggcg gactgggaca agccgttcat tgcgatcagc aattcctaca
     3841 ttgattgcgt tccggggcat gtccatctca atgaggtggg cctgctgatc aagcgcctgg
     3901 tccgcgcggc cggcggcgtg ccgttcatct tcaacaccat cggcattgac gacggcatcg
     3961 ccatgggcca cggcggcatg aagtattcgc tgccgtcccg ggaactgatt gccgactgcg
     4021 tggagtccat ggtccgcgcg cattgtttcg acggcatgat ctgcatcccc aactgcgaca
     4081 agatcgtgcc gggcatgctc atgggggcca tgcggctgaa tatcccgacg attttcgtca
     4141 gcggcggccc gatggtggcc ggcattgacc agcaggcggc ggatgatccc ctgccccccc
     4201 acctgcgccc ggccacgcaa tcctccgacc ttgtctcggt gttcaaaggc gtgggcgaat
     4261 tgcaggcggg caagatctcc cggcaggact tgcagcacct ggagcaaagc gcctgcccga
     4321 cctgtggttc ctgttcgggc atgttcaccg ccaactccat gaactgcctc agcgaggcgc
     4381 ttggcatggc gctgccgggc aacggcacca tcctcgccgt ttccaaagaa cgcgaagccc
     4441 tctacgagcg cgcggcccgc gcgatcctca aactcgtcga ggccgacctc aagccgcgcg
     4501 acatcgccac gctggaagcc tttgacaacg ccatcgccct cgatgtggcg atgggcggtt
     4561 cgaccaatac catcctgcac acgctggcca ttgcgcgcga ggccggcatc gcctacgaca
     4621 tcaagcgcat tgacgacatt tcgcgccgcg tgccctgtct ttgcaaggtg tcgccctcct
     4681 cgaattacca tgtccaggac gtgcaccggg ccggcggcat tcatacgatt ctgggcgaac
     4741 tcaaacgcat gggcgcgctc accctgagct gcaaaacggt caccggcaag accctgggcg
     4801 aaaacattga cgaatgggac gtgcgctcgg aaaagtgcgt tcgctgggcg aaaacggcgc
     4861 gggtttccgg ctgctcggcc attgtggtgg acccggccga caagctcggc catgccgcgc
     4921 gcacggtgag cggcaacctg gcgcccaaac ccttgctctt tttccccggc gacccgcgcg
     4981 gcataacgct ttggcgcttc gccgccggtt tcaacgcggg cgatgccgcc gccatcgccg
     5041 ccctgttcag cgcggagggg gaattgcagg tggacgaaac caccacctgg aaaggcccgg
     5101 cggcgatcga ggccggactc aagcaagccc tggccggggc gaaaggcctg ttgcgcgccg
     5161 aactgggcca gctcgacgga acgccgctgc tgatcatctg gaaatacggc aaaggcgccg
     5221 ccaagcaggc gcactgcctg ttgcgcgccc cggggttgca agattggcag attcagcggt
     5281 tggagcacga ttaccggccg gaggcgatca aagccgccca accctcgggc ctgatgcctt
     5341 acgatccggc gttcctgttc aatgcccggg attgcatccg cacccgggaa accgcttaca
     5401 ccgccgacgg cggcctggcc atcctttacg gcgggattgc gcccgaaggc tgcgtggtca
     5461 agaccgcggg catcagcgac gaattcaagc aatactgcgg gccgaacttt gtttttgaag
     5521 gtccgtgcgt gatctttgaa agccaggagg acgcctgcgc cggcatcctc gccggcaaag
     5581 tcaaggcggg cgacgtggtg gtcatccgca acgaaggccc gcgcggcggc ccgggcatgc
     5641 aggaaatgct ctcccccacc agctatattg tcgggatggg cctcggggat aaggtcgccc
     5701 tgatcaccga cggccgattc agcggcggca cgtccggcgc ctgcatcggg cacatctcgc
     5761 ccgaggcggc cgaaggcggc cccatcgggc tgttgcgcga gggcgaccgc ctgcgcattg
     5821 atttccccaa tcgccgcctg gacgtggtcc tgccggaagc ggaactggcg cagcgcaaga
     5881 aaacctggca gcccgtcaag cgcgacctgt ccggctggct ggcgcgttat caaaagctgg
     5941 tcagcaacgc cagccagggc ggcatcctga ccagttgaat gcgagcacgg ccgtccgggc
     6001 aggctgtccg gctggactga taccgcggcg cggcacggtg tagatagccg ggccggggac
     6061 tgtcccaacc cggcttgcag gacaagtcac cgtcccggaa atggacaggg gcgatggctt
     6121 tgcgggtgct ttgaaacgaa gcctttcagg ttgtccgcgc aacccaccaa gtgcgctaaa
     6181 aaaaagggcg aaaacgggaa cggcgcgcaa tctcatccgg ccggcaagcg atgagccggc
     6241 ggcacgcgga ttaattggca attttccagg catttagcgt gttcggcgtg tcctttcaaa
     6301 gcccgaatat aaggccaatt gcggccgtta gatccgcttt ggttctaccg cttttgggcc
     6361 aggggaatcg agctaacctt cgcagttatc tccaggaaga tcggttgccg agcgcgtgcg
     6421 cgcgcccgaa atcatctttg gatcggactt ggcatgtaag ctgcaattaa aagatttgta
     6481 ggttccctgc ctacatcctc ctgagagggg cggagccggg accaacaggc actgcccttc
     6541 tcttttcatg gttgggttgg gggcggcccg gagtaagagc cgggccgctc tttttgtacc
     6601 cagttccgca cgattccagg ctgcccctgt ccgccctggc agcttcaacg ccgccggagc
     6661 cccggggcga ggcgcaccgt tccgcgcccg gaaatggcgc gcttgctggc gcacggatgg
     6721 agaagtgaaa ggagcttaat aacggcctca accgacgctg gaagtgaagc ctgcattccc
     6781 ccaaaaagcg ggattggcca acgcataaaa aggtcagtta atccccttga ccggcagcgg
     6841 gtctttccca tagtctctcc gcttgttagg caaaattcat gtttgcacaa cttaagagcc
     6901 tgttttccaa tgatattggg atagacctcg ggacggctaa ttccgttgtt tacgtgcggg
     6961 atcagggcat tgtcttgcgc gagccctcag tcgtcgcgat tcaggcggga accacgaatg
     7021 tccttgccgt aggcgaagaa gccaagcgga tgctggggcg caccccgggg aatatcgtgg
     7081 ccatccggcc catgaaagat ggcgtgatcg ccgacttcga gattaccgaa gcgatgctgc
     7141 gccactttat ccagaaggtt catcatcgga aactgattgc gccgcgggtc gtcgtcgcgg
     7201 tgccttcggg tattaccgaa gtggaaaagc gggccgttaa ggactccgcc actcatgccg
     7261 gcgcccggga agtgtatttg atcgagcaac cgatggcctc ggccatcggc gtcggactgc
     7321 cggtgcatga gcctgcgggg aatatgatcg tggatatcgg cggcgggact tgcgagatcg
     7381 caattatttc cctggccggc atcgtcttca gccgcagcct gcgggtgggc ggagacgagt
     7441 tcgacgagac gatcgtggca cacattaaac gagcctataa cctgatgata ggcgaacgca
     7501 ccgcggaaga gatcaagatg cgcattggct cggcttttcc gctggagcag gaattaacta
     7561 tggaagtcaa gggccgcgat ttgagcgcgg gtctgccgaa gacgctgacc atccgctcgg
     7621 aagagattcg cgaggccttg caggaaccgt tatcgagtat tttggagtcg gtacgcatta
     7681 ccctcgaacg ctgcccgccc gagttgtccg ccgacctggt ggaccggggc atcgtcatgg
     7741 ccggcggagg atcgttgttg cgcggcatag accggctggt ggcggaagag accgggttgc
     7801 cggtccataa ggcggatgac ccgctcagcg ccgttgcgga aggcaccggc cgggtgctgc
     7861 aggaactcca ttttcttaag cgcgtttccg ccggttccaa ttagttcccc cctcatcatc
     7921 acggtggctg gcagtcggca tcggtgaacg gtcgcccacg acctttcccc agccgccagc
     7981 cgccgctcgt tcattgccga cgagttagat gttgaaacgg tcgcattata ttacattggg
     8041 cctggtggtg ctgctggcgc tggtggtttt gaaccttccc ggccaaacgg cggcccgcct
     8101 caagctcgcc ctgggcagcc tgtttctgcc cctgttcggg ctggccggct ccacgcagca
     8161 ggtggtcggc gccgccgcag acgccatcct gccgcggcgg gaactggtca agcagaatgc
     8221 gcaactgcgc caggaggaac aacgcctgcg cctgcaggcg ttgcaggcgg cggaaatcgc
     8281 cggcgagaat gcccgcctcc ggcaactggt gggatggcgc gcccagaccc cgtggaagct
     8341 caagctggcc aaagtggtcc tgcgcgagcc ggccaactgg tggcgctcga ttcagattga
     8401 cctgggcagc cgcgacggtc tgcaagtgaa cctgccggtc ttgaccacgg agggcctggt
     8461 gggccgggtt gcttccgtga gtttgacccg gtcgcaagtc gttctgctgg gagacccgaa
     8521 ttgcaaggtg gccgcgcggg ttgaaaacga aacccgcgac accggcgtaa tcagcgcgga
     8581 cggccccctg gcagctcaat tagtgcggat ggattatctt tcccggggcg cggaactgaa
     8641 gcccgcgcaa cgggtgttca ccagcggtga ggggggcatt tttccgaaag gcatccccat
     8701 tggccaaatt gtggatgtgc agccggtgga ctacggcttg cgctacgagg cgcgcgtgcg
     8761 gctggcggcc aacttaaacg cattggaaga ggtgtgggtg atgtttccat gaactggctc
     8821 aataccgctt ttatcctggg agccgctttt ctggcggtat tcggacaggc cgcgttcggg
     8881 agcgcgcgct ttctcgcggg cgcgcaagtg gacctgctgc cgccgctcat ggtttatgcc
     8941 gccctgtcct cggggctgac caccgtgacg ctgctggcgg tgtgcgcggg gctgtggttc
     9001 gactcccttt ccgccaatcc gctgggggta accgtgctgc ccttgttcgc cgtcggatgg
     9061 gtgattttcc tcaaacgcga attgattctg cgcgaccagc cctttgccca ggcggtcctc
     9121 ggcgcggcgg ccagcgtcgt cgcgccggcg ctgacgctgc tgctgctgct gaccatggga
     9181 agccatccgc tgttcggctg gggcacgctg tggcaattgg tcgtgctctg cgccgggggc
     9241 gccctcgccg cgccggtttg gtttgaactg ttcggctggc tgcagcatgc cctggttcac
     9301 cgcggcgcga ccgaatccag tttccgcccg gatcgggaaa tccggcgagg ccgccgcgga
     9361 gccttgatcg gcggctaccg cggcgaagtc atagaccggg cgagaagcaa accgaggcgc
     9421 ccccgatgaa catccctttg gcccatcctc cctttagccc gcgctttccg aactgatgtt
     9481 catcttcgac caactccgaa ccgacgatgc ccacctgcgg gcggtggcgg tgaccgtgct
     9541 ggccggattg ggcatcctgc tgggcgggtt gtggtgggtg caggtcgtct cggcggacga
     9601 ataccggacc aggctcgaac ggcaatcctt ccgcacggtg cgcatcccgg cggtgcgcgg
     9661 ccagatcctg gaccgcaacg gccgggcgct ggcggaaagc cgccccacct acaacatcag
     9721 cctctactgc gaggaacttc acgacacatt cgacgcggtg tatgcggaga aagccacccg
     9781 cgcccgcgcc acgctgaaac tcgcgctgca ggaacgggag agccagttgc atcgcaagct
     9841 caccaaggcg gagcgccgcc ccttcctttt taccgcggcg caccgaaacg cgttgcggcg
     9901 ggaagcccgc tgcgaagtgg ccagcaacgt ggtggcccag gtcagccagc ggctgcggca
     9961 gccgctctcg ctggccgtga ccaacttcga gcagcattat caaaagcgcc tcgccctgcc
    10021 ctacccgctg ctgacggatc tgacgccggc gcaaattgcc cggttcgagg agcaatccac
    10081 cagccccctg ggcgtggacc tggaaatcct gccgacccgc tgctaccctt acggcaccac
    10141 ggccacccac gcactcgggc acctgctgcg cgacgacagt tccgtggagg gggaggaagc
    10201 ctttttctcg taccgcctcc ccgattaccg cggcgtggtc ggaatcgagt acggcttcga
    10261 taaagaactg cgcggggtag ccggcgccaa atcggtcctg gtcaacaacg tcggctatcg
    10321 ccagacggag aatatctgga gcccggcggc gcccggccgc aatgtggtgc tgaccctgga
    10381 tttggcggtg caacaggccg cggagcgcgc gctgcaaggc gtcgcgggca gcgcaacgcg
    10441 gggggcggtg gtggtcatgg acgtgtggac gggcgacctc ctggccctgg tctcatcgcc
    10501 cacgctggac ccgaactggt atgtgcgggg catcacccac gaacaatggc ggcgcatcac
    10561 cgagctgaac gccgaaaaga atcgcgccac gtttgaaaac tacatgccgg gctcggtctt
    10621 taagaccgtg gtgggaatgg ccgcgctgga aagcgggttg aacccggagg aaatcatcca
    10681 tgtcgagccc aaccccgcgc agcccgccaa aggccacaaa cgcgtcggca aacgcacgtt
    10741 caaggacctg gccccgcccg gcgactataa cttccgccgg gcactcaaac tttccagcaa
    10801 ctcttatttc atcacctgcg gcctgcgggc cggccccgag cggatcatcc gcctgggcca
    10861 gcggctgcac ttcggcgagc gctccgggct gcccacgcgg caggatgcgc ccggccagtt
    10921 ccccagcctg cagcggctcc gcaccgactg gagcgatggc aacaccggca acttatgcat
    10981 cgggcaggac ccggtctggg tcacgcccct gcaaatagcc gtcctgaccg cggccatcgc
    11041 caacgacggc attgtcctgt ggccccggct ggtggaccgc atcgaatcgg tggacccggc
    11101 ctcgccgcag ccgccgcagg tcttcccgcg ggggcgggtg cgcgaccacc tgggcgtgag
    11161 cacgcgcacg ctgcgcatcc tgcgcgacgc catgctggcc aacgtggaag acgccgacgc
    11221 cacgggccgc ggagccgccg tcccgggaat ccgaatcggc ggcaaaaccg gcacggcgca
    11281 ggtgcaggac gcccgcaacg tcaagattgg ccagacaacc tggttcgcct cgtttgcgcc
    11341 ttatgaacaa cctcgatacg ccgttgtcgt gatggtcgaa aacggcgttt ccggcggcac
    11401 cacctgcgcc ccgatcgccg gcaaaatcta ttccgccctc ctcgaccgcg agcgggcgca
    11461 actggcctcc tcaccggccc tggcccgaaa cccctgatgc gcgcctcgcg gccctaacca
    11521 tgtttgacca ggacctccag cgtcacgaat cccgcataga ccggctccaa atcgccgccc
    11581 tgtgcgggct gatgctgctg ggaacggtct ttgtctatag cgcgaccatg gtcggcgaat
    11641 ccgccgccac gctggtgtgg tacaagcaga gctggttccg gcaagccgtc tggtatgggc
    11701 tgggcatggg cgccgccacc gcggtttgct gcgtggatta tcacatcctc gcccgctggg
    11761 ccatggtcat ctactggagc gcgatcctga tgctggtcct ggtcttgttc ttcggcaccg
    11821 tgcgcttcgg cgcgcgccga tggttcgacc tgggcttctt cagcctgcag ccgtcggagt
    11881 ttgccaagct cgctttcatc ctggcccagg ccaactacct gagccggccc gtcgaagagt
    11941 tacgcgggct ggcgaccttc tggaagtcgc tcggactgat gctcctgccg ttcctgttga
    12001 tcctgaagga gcccgacctg gggtccgcgc tggtgttcct gcccaccggg ctggcgatcc
    12061 tgttcgtggc cggaatcccc cggcgctacc tgctgcggct gctcggaggc gcgggcctcc
    12121 tgggcgcgct gtttgtgggc aacgtgctgt ttgcgccccc caactggcag atcaaactcg
    12181 aagactacca gcggcggcgg ctgctggtgt atttcggccg ggaagaagcc cccccgccgg
    12241 gcgcgtcccg ggcggagctg gcccgcttgc gccagcaaca ggcggattac tcgcataacg
    12301 tgcggcaggc gctgatctcc gtgggctcgg gtgggctcat cggcaaaggc tggcggcaag
    12361 gcacgcaaaa cgccctgggc ttcctgcccc gcccggtggc gcataatgat ttcattttct
    12421 ccgtcattgc cgaggagaaa ggattcgtcg gcagcgtgat agtgctcacg ctctacgggg
    12481 tggttctgtt ctcgggaatc agaatcgccg gccaggcgcg cgatcgcctg ggcaagatga
    12541 tggccgtggg cgtcgttgcg ctcatcttca gccacgtctt catcaatatc ggcatgaata
    12601 ttcggttgat gcccgtgacg ggcgtgccac tgccgctgct gagttacggc ggatcttcag
    12661 tgctcggctc gttgatcgct atgggcatcc tgcaaaacgt gtacttctat cgcagaacct
    12721 attaattatg agtgacaaaa acttctcccg ccgccggcgc ggcatgcggt ttcgccccag
    12781 ccgcggcatc ggccacgggc aaccgcaaac gcccgaccgc gtcggcaacc aggcgcgcgc
    12841 cgaggccgtg gccccgccgc cgatggaaaa gatttacgat cgccgccacg accacgaaat
    12901 tgaccgggcg gagaacatcg ccgccggcct gccgccggag ggcccccgca aatccccggc
    12961 cgcgccggat gctccgggcc aatcccccga atcaaccccg gcgccccctc cgcgcggcga
    13021 acaagagaaa cccttcaccc cggtgcccat caaggagccg tcccggggca ttgtggaaac
    13081 cctccgctcc gcggccaaca acctcgtcaa aaaggttcag cggctgatca agcccgtcaa
    13141 gcgcgtccac aaggaagtcg tcatcaacgc ggaaaccctc gaaacccgcg tcgccgtctc
    13201 cgaagaaggc cgcctcgaag agttcaccat cgaacgcacc accgaagagc ggctggtcgg
    13261 cagcatcttc aaaggcaagg tccgcaatct ggaagacggc ctcaaagcgg cctttgtgga
    13321 catcgggttc gagaagaacg cgtttctgca ctactgggat attgtcccga gcaactttga
    13381 cagcggcgtc gagatcgtgg accgcggccc ccgccggcgc gacaaaccca aaattaccca
    13441 gaaagacatc ccccgcctct acccgccggg cagcgaaatc atcgcccagg tgaccaaagg
    13501 ccccatcggc accaagggcc cccgcgtcac gaccaacctg gtcctgccgg gccgttacct
    13561 cgtcctgctc cccaactcgg accaaagcgg catctcgcgc aagatcgaaa atgcccagga
    13621 acgccagcgc ctgaagaaga tcctgcgcga actcgccatc cccgacggca tgggcgtcat
    13681 catgcgcacg gtgggcgaag gccagcaaaa acggtatttc gtgcgcgacc tcgccctcct
    13741 gctcgaggag tggcgccagg tccaggaacg catccagaaa cagcccctgg ccagttgcgt
    13801 attccaggaa cccgacctga tcgagcgcac cgtccgcgac ttcctgaccg aagacgtcga
    13861 acgcatcgtc gtggacagcc cgaaagccca cgaccggatg cgcgaaatga ttgccaaaat
    13921 ctccaagcgc tccgctgaca aggtcaaact ctacaccgac ccgcagccca tctttgaccg
    13981 cttcaacatt acgcggcaac tggagaacgc cttctcccgg caggtccacc tcaaaagcgg
    14041 cggctacatc gtcgtggacg aaaccgaggc gctggtcgcc attgacgtca actccggccg
    14101 ccacaaaggc ggcaaagacc aggaagcggc catcctcaag gtcaacctcg aagccgccga
    14161 cgaaatcgcc cggcaattgc gcctgcgcaa tatcggcggc ctgatcgtgc tcgacttcat
    14221 tgacatgaaa tcccgccgcg accaacagca ggtttaccag cgcatgaagg aagcgctgca
    14281 ccgcgacaag gccaaaactc acgtcttgcc catctcgcag ctcgggttga tggaaatgac
    14341 ccgccagcgc cactccgaaa gcgtcaacgc cgccgtctat gacgactgcc cctactgcaa
    14401 aggccgaggc aaggtcaaga gcgccctgac catgagcgtc gaaatccagc gcaagctgag
    14461 cgaaatcctc aaaaaacgcc cgcgcgacga gtcggacttc caactgcgca tcgcggtgca
    14521 tcccaccatc ctcgaacggc tccgcacgga agacgaaaaa cacctcatcg aaatggagaa
    14581 acgctacttc ggcaaactct ccttccgtgc cgacccgggc ctccacgccg aacagttcaa
    14641 aatcgtcaac gtcgccaaca acgaggaact gggcaacgcc agcaagtaat accacttctg
    14701 gcacgtaaat gcactattct ccgctgcggt agcgtttac
//
LOCUS       DAOPQG010000088        14564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4857_length_14564_cov_14.568199, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000088 DAOPQG010000000
VERSION     DAOPQG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4857_length_14564_cov_14.568199"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PKI07_10205"
     CDS             <1..713
                     /locus_tag="PKI07_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51462.1"
                     /translation="FEIFIAGMECGDNWSEQNDPAHLLETWRRAYRAEERDSGKFHTL
                     DFDFIEALEYGMPPTTGIGPGVERMAMIFTEQEIIDDVIFFPMMRPLVAPVNAAIYGV
                     AEPALAPVEDLALTVPDFEALCREGLLRPHARNLALKPHVRQWGLSEGRPRTSVHVDI
                     EGFLAGSVLRLAGGSLPAGEAALKPGGEHGVAGAGAPAGPEGSRPESAELPLARFLRD
                     TFPDCQVTVSPATVLRHG"
     gene            complement(726..1835)
                     /gene="dnaN"
                     /locus_tag="PKI07_10210"
     CDS             complement(726..1835)
                     /gene="dnaN"
                     /locus_tag="PKI07_10210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOC51463.1"
                     /translation="MKLTIAKEQMINGLQAVQNVVSARTTLPILSNVLLRASEQHLEF
                     TATDLDVTISCAVEAKIKTPGAATVPVKKLFGIVRELAALELDLEVDDKNICAIHAGS
                     SFYKIHGLSADEFPPMPQFKEDKKVVVPQETFKGMMRKTSFAISNDESRYVLNGVFIS
                     LKDHKMTMVATDGRRLALVDEEVDIAEKNQGEFIIPTKTVLELNRLLQEKGTVEVRYA
                     ENQASFTLKDEKTSPILVITKLVEGNYPNYRQVIPAETKERVTLVREEFLHALRRAEI
                     MTTEKSNSVKLSFTNNKLEITVNSPDVGEAKETMALNYKGREITIAFNPKYVIDPLNA
                     LTHDEVFLELIDDLSPGVLKINGPFLYVVMPMRLT"
     gene            2157..2846
                     /locus_tag="PKI07_10215"
     CDS             2157..2846
                     /locus_tag="PKI07_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOC51464.1"
                     /translation="MFQPADLFDLQQTAHPEVFAGCEYAWDALKRLAAYLEAGRFPNP
                     AGRRGGAVFIGERVFIGEGTEIEDGAMIKGPAIIGRNCQIRHNAYVREHVIVGDNCVV
                     GNACELKNALLFNGVVAPHFNYIGDSILGHRAHLGAGVKISNVRFGPGNVTVEMNGQP
                     YDTGLAKFGALLGDGADIGCNAVLNPGSIIGRGAVVYPNTNWRGILPAHMMVKNRAAQ
                     EVVVRRPRESG"
     gene            2874..5015
                     /locus_tag="PKI07_10220"
     CDS             2874..5015
                     /locus_tag="PKI07_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOC51465.1"
                     /translation="MISVIESQPAGSGTDLVRQTADIFSPTGLLSQARNFEFRPQQQE
                     MAVAVARALDAGEHLAVEAGTGVGKSFAYLVPAILYAVANRKKAVIATHTINLQEQLT
                     QKDLPVLAQVLPVKFSFAMLKGRANYLCTRRLRKALQQAPNLFTTSETQELQRIAEWA
                     RTTEDGSRSDFDVEPDLKVWDQVCSERGLCAPKLCGYPSDFAREHGVCFFQRARQRIL
                     SADVVVLNHTLLFTLLGGQEESADGGILFRNDFVIFDEAHQMEHEASKHIGLTVSSGQ
                     VRYALHRLWNPRTEKGLLATLRKGAAVKLVAELLAAADGFFASVEHACDELLPASRKR
                     IGGGGDASGARRREWTELRIRRPELVPDKVTLPLQRLREAVADLIKLSEDSEMGLELM
                     EGNRRLAELREGIAAFLSQSAADHVYWVERTGRAQKTLALHAAPVDVADYLRQRLFLP
                     GTSVVMTSATLSVSGREAPAVSDDAPPRGARSAGGAPPGAGLDYFVHRVGAEAAVRLQ
                     VGTPFDFERQMKLCVVRQMPDPRTEDYRDALIHWIEHFVKQTHGKAFVLFTNFKLMQE
                     VGELMQPFFARLGLACFVQGTGIPRSLMLEKFKEDVDSVLFGTDSFWQGVDVPGAALS
                     NVIITRLPFAVPDHPLIEARIEAIEARGGNSFRDFSLPEAILKFRQGVGRLIRTKTDQ
                     GIIVVLDNRLLAKQYGQAFIQALPRCPVEVV"
     gene            5072..5797
                     /locus_tag="PKI07_10225"
     CDS             5072..5797
                     /locus_tag="PKI07_10225"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC51466.1"
                     /translation="MTSDKYSPPKYFQICRRIVGAIQRGELPPGAPVASENEIIERYG
                     VSNTTARKVLHELEQEGWVTRVKGKGTFVRDFPVLRSINRIFGFTRNMIEAGRKPATR
                     LLGFHLREGDHSQTINGRLYTLKGPFCEIERLRLADGIPMMKETRYLSLSLCPNIHRR
                     NLEQSLYQIFDKHYGIRLTEINQMLSAVVLEGEWLPVFQVDRPIPAFRVEGASFCGKD
                     LIVEMENSVYRGDRYRFAAKAVR"
     gene            5807..7318
                     /locus_tag="PKI07_10230"
     CDS             5807..7318
                     /locus_tag="PKI07_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOC51467.1"
                     /translation="MIGDLWAASAGDLAHHAGKLALLGLDLLLAGVASGHAILYKRDS
                     RSAIAWVGFIWLVPLGGAALYFILGINRIRRQAVLLRRGLERYRAHAALPACPPDELP
                     HHLPGHTGHLNMLARVVNGVVARPLLPGNRIEPLLDGDEAYPAMLEAIRQARRTVAFY
                     TYIFDRDAAGLAFARALGEATRRGVEVRVLIDAAGTRYSWPTILRALRREGVRFARFL
                     PSLALWHLMAVNLRTHRKILVADGRLGFTGGMNIRAGHCLKGFGGGAGDGPARPPRHP
                     VRDIHFRVRGPVVAQLQEVFVDDWLFTTGELLRGEDWFPHPEPAGPVLARGVPDGPDE
                     DFEKLRWTLLGALAIARYAVRIVTPYFLPDPALISALNVAAKRGVRVDIILPAKNNLP
                     FVGWASRAMWWQVLEHGCHIWLTPPPFDHTKLMLVDGCWVLLGSANWDPRSLRLNFEY
                     NLECYDAELARRLEEIVAARIATARPVTLADVDARSLPIRLRDGLARLFTPYL"
     gene            7392..8549
                     /locus_tag="PKI07_10235"
     CDS             7392..8549
                     /locus_tag="PKI07_10235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC51468.1"
                     /translation="MKVPLLDLKAQYAAIKPDLDAVVAEVMVSQQFILGPQVEACERA
                     IAQYSRCAFGVGVSSGTDALIICLMAEGIGPGDEVITSPYTFFATAGSIARVGARPVF
                     VDINPATYNLDVAQVEARITPRTRAIIPVHLYGQMADMEALMPIAQKHQLVVIEDAAQ
                     AIGAEHHGRRAGSIGHYGCFSFFPSKNLGAAGDAGMVVTQDAARAGKLACLRMHGSQA
                     KYYHQVIGGNFRLDALQAAVVRAKLRYLDAWTAARQRNAERYHRLFTEAGLPLAGSGE
                     YRARAGDASVLFLPDVATDRHIFNQYVIRTPRRDALRAALQARDIGTEIYYPVPLHLQ
                     ECFAYLGGRPGTLPESEKAARETLALPIYPELSEAQARQVVQSITEFMSAS"
     gene            8553..10232
                     /locus_tag="PKI07_10240"
     CDS             8553..10232
                     /locus_tag="PKI07_10240"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOC51469.1"
                     /translation="MNPIPSPEIPPLRGERILRDPAQNKDAAFTAAERRQLGLEGLLP
                     PAVLTLAQQVELELEHFHQKTDPLEQYIGLMALLDRNEVLFYRLLIENLDRLAPIIYT
                     PTVGQACQRFSHIFRRPRGLFLCPADRGQMAARLGQLRHRDIRLIVVTDNERILGLGD
                     QGAGGMGISIGKLVLYSAGAGIHPRLTLPISLDVGTDNAALLEDPLYCGYRARRLRGP
                     EYDAFVEEFVQAVKHVFPRALLQWEDFKKANAFRLLDRYAARLPSFNDDIQGTAAVAL
                     AGIFTGLRLTGKTLREQRFLMAGAGAAGVGIGRLVRTAMLEAGLTEAEIRPRQIFLDS
                     GGLVWEGRADLEDHKRAAALRKDELALLGLSTPPPAALAEIVRAVRPTILIGTTGQPG
                     DFTPAVLRAMADHCERPLIFPMSNPTSKTECTPAEALQHTDGRALVATGSPFDPVVFN
                     GRKYVIGQCNNAFVFPGVGLGALIAEASRVTDSMFLAAAQALADFTVQHPAGENCLYP
                     SLQQLRAVSRLIALKVAQTARHEALGRNLEDAALQAAIDQFFWHPDYPPAS"
     gene            complement(10335..10802)
                     /gene="trxA"
                     /locus_tag="PKI07_10245"
     CDS             complement(10335..10802)
                     /gene="trxA"
                     /locus_tag="PKI07_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC51470.1"
                     /translation="MKLLRYRSSLILGGALLLALAVLPACTKMKKADAETTAPDPDKP
                     DARVVVTGASFDQLLKESTKPIVLDFWAPWCGPCRKMDPIFAAAAQERPDIIFGKVNV
                     DQEHKLANKFGIQGIPTLLIFVEGKMVKQSVGLISKAELLSLLDSTLKKHSKS"
     gene            10962..11573
                     /locus_tag="PKI07_10250"
     CDS             10962..11573
                     /locus_tag="PKI07_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOC51471.1"
                     /translation="MNKSIEIMMREHKLIVGVLASLQALADKLAAGETVAREDVADFG
                     RFFRDFADQCHHGKEEDRLFVQMVEAGLPRDRGPIAVMLAEHDAGRQEVRGLIEVGAG
                     AGPLSESERARVVDYARRFVPLLSSHIHKEDHILYPMAQETIPAPAFAALDQACEDFD
                     RQVRQQLDVPGLEDLAARLMERYPACLGQLSDDHRCGACSAAG"
     gene            complement(11583..12275)
                     /locus_tag="PKI07_10255"
     CDS             complement(11583..12275)
                     /locus_tag="PKI07_10255"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC51472.1"
                     /translation="MKTRINGLGRLWVCGGMILWGAVSCSTPAGRPTGLELFNGKDLA
                     DWKSVLADPAVPMDQVWSVQDGILTCQGTPLGFLYREPAAGNFRLAVEYRWPPGHTPG
                     NSGIFTRLNAPFSPLPCAVEVQLKYGNAGDVFGLQGKPIARGQPRFFEIRQHPEGGDI
                     AGISKLLEAENPPGEWNRVEILARGERYRVWLNGKLVNDVSGVHRGAGLIGLQSEGGV
                     IQFRRVTLQPLD"
     gene            12450..13556
                     /gene="dprA"
                     /locus_tag="PKI07_10260"
     CDS             12450..13556
                     /gene="dprA"
                     /locus_tag="PKI07_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOC51473.1"
                     /translation="MEPHEALVALNLIERVGPVRVRQLLEQFGEAPAILRASRRQLLQ
                     ARGIGEDIADAIVNWEKTTDLAAELKRIAAFGCRIVTQADAEYPELLRQIYDPPVVLY
                     VKGRLHAQDKNSVAVVGSRLTTPYGLETARKLARQLAALGVTVVSGGARGIDTAAHQG
                     ALSGQGRTLAVLGTGINLVSPAENAKLFGQIADHGALLTQFPFNRPADRQSFPIRNRL
                     VAGMTLGTVVVEANLTSGALITANFAADYGRQVFAVPGRIDSPRSKGCHDLIKKGAKL
                     CEGVEDILSEFEYLFPPASPPSSPAAAGPRPAVPLNENEQKVLGLLGPEESHIDEVIR
                     RSGLPSSAVSVALLSLEMKRLIRQLPGKLFARNG"
     gene            13928..14203
                     /locus_tag="PKI07_10265"
     CDS             13928..14203
                     /locus_tag="PKI07_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51474.1"
                     /translation="MKFAIEIGDTEKHRLEYNFNQLLGSLVIKVNERPIKKSVRLINE
                     PVLEVHAFIVGDREKSSVRIEKERMPLLGGRNRIYVNDRLVRVFSGI"
BASE COUNT         2418 a         5052 c         4635 g         2459 t
ORIGIN      
        1 gcttcgagat attcatcgcc ggcatggagt gcggcgacaa ctggtcggag cagaacgacc
       61 cggcgcacct gctggaaacg tggcgccggg cgtaccgggc cgaggagcgg gacagcggga
      121 agtttcacac gctggacttt gatttcatcg aggcgctcga atatgggatg ccgccgacca
      181 cgggcattgg gccgggcgtc gagcgcatgg cgatgatttt taccgagcag gagattattg
      241 acgatgtcat cttcttcccg atgatgcggc ccctggtggc gccggtgaac gccgcgatct
      301 acggggtggc ggagccggcg ctggcgccgg tcgaggacct ggcgctgacg gtgccggact
      361 tcgaggcgct gtgccgcgag gggcttttgc ggccgcacgc gcggaacctg gcgctcaagc
      421 cgcacgtgcg gcagtggggc ctttccgagg gccggccgcg gacttcggtc catgtggaca
      481 tcgagggatt cctggccggc agcgtcctgc ggctcgccgg aggttctctg ccggccggcg
      541 aagcggccct gaagccgggc ggcgagcatg gggttgccgg ggccggcgcg cccgccgggc
      601 cggagggttc ccgcccggaa agcgcggagc tgccgctcgc ccggttcctg cgggacactt
      661 tcccggattg ccaggtcacg gtttcccccg cgaccgtgct gcgccacggc tgaccggcgg
      721 cggcgtcagg tcagccgcat gggcatgacc acgtagagga aagggccgtt gattttgagg
      781 accccggggc tgaggtcgtc aatcagctcg aggaaaacct cgtcgtgggt gagggcgttc
      841 agggggtcaa tgacgtactt gggattgaag gcgatggtga tttcgcggcc cttgtaattg
      901 agggccatgg tttccttggc ctcgcccacg tcgggggaat tgaccgtgat ctccagcttg
      961 ttgttcgtga agctcagttt gacggagttg gacttctcgg tggtcatgat ctccgcccgc
     1021 cgcagggcgt ggaggaactc ctcccggacc agggtgacgc gctctttggt ttccgccggg
     1081 atgacctggc ggtagttggg gtaattgccc tcgaccagct tggtgatgac gaggatgggg
     1141 gacgttttct catccttgag ggtgaacgag gcctggtttt ccgcgtaacg cacctccacc
     1201 gtgccttttt cctgcagcag ccggttcaat tccagcacgg tcttggtggg gatgatgaac
     1261 tcgccctggt tcttttccgc gatgtccacc tcctcatcca ccagcgccag gcgccggcca
     1321 tcggtggcca ccatggtcat cttatggtct ttgaggctga tgaaaacccc gttcagcacg
     1381 tagcgcgatt cgtcattgga aatcgcgaag gaggtcttgc gcatcatgcc cttgaaggtc
     1441 tcctgcggca ccacgacctt tttgtcctcc ttgaactggg gcatgggcgg gaactcatcc
     1501 gcgctcaagc cgtggatttt gtagaaggac gagccggcat ggatggcgca aatatttttg
     1561 tcgtccactt ccaggtcgag ctccagggcg gccagctcac gcacgatgcc aaagagcttc
     1621 ttgacgggaa ccgtcgccgc gccgggggtt ttgattttgg cctccaccgc gcaggagatg
     1681 gtcacgtcca ggtccgtggc ggtgaactcg aggtgttgtt ccgaggcacg cagcagcaca
     1741 ttggacaaaa tgggaagcgt ggttcgggcg cttacgacat tttgcactgc ctgcaaccca
     1801 ttgatcattt gctcctttgc aatcgtcagc ttcattacgt gcttaatata tctgtgttta
     1861 tctttgaatg actatccgta ttaagggccg tgaataaggc caataacccc aatagtcaac
     1921 gccagcgcaa caacttacgc aagcatgaaa cgtgaatgca attcccaatg ccagtgaaaa
     1981 aagcggcgga tgccggggtt gttcactttg cccacaagtt atccgtattc ccttccgggc
     2041 tttttcaccg ggtcattcac accggcggcc cggcggccgg ggcgggcgcc gtttccgggg
     2101 gcggcgcggc taaaatcggg tttggttccc ggcccggatt cgcctaagct gtcggcatgt
     2161 tccagcccgc agatttgttc gacctgcagc agacggcgca tccggaggtc tttgccggat
     2221 gcgaatacgc ctgggacgcg ctgaagcggc tggcggcgta tctcgaagcc ggccggttcc
     2281 cgaacccggc gggccgccgc ggcggggcgg tgtttattgg cgagcgcgta tttatcgggg
     2341 aaggcacgga aatcgaggat ggcgcgatga ttaaaggccc ggccatcatc gggcggaatt
     2401 gccagatccg gcataatgcc tacgtgcgcg agcatgtgat tgtgggcgat aattgcgtgg
     2461 tgggcaacgc atgcgagttg aagaacgccc tgctctttaa cggggtggtc gccccgcact
     2521 tcaactatat cggggattcc attctcggcc atcgcgcgca ccttggggcc ggggtgaaga
     2581 tttccaacgt ccgcttcggg ccgggcaacg tcacggtgga aatgaacggc cagccctacg
     2641 acaccggcct ggccaagttc ggcgcgctgc tcggggacgg cgccgacatt ggctgcaatg
     2701 ccgtgttgaa cccgggcagc atcatcgggc gcggcgcggt cgtgtacccg aacaccaact
     2761 ggcgcgggat tctgcccgcc cacatgatgg tgaagaaccg cgcggcccag gaggtcgtcg
     2821 tgcgccggcc gcgggaatcc ggctaagccg cccgcgcccg ccgcccgctt cgcatgatca
     2881 gcgtcattga atcccagccc gccggttccg ggacggactt ggtgcggcag acggcggata
     2941 ttttctcccc gaccgggctg ctttcgcagg cccggaactt cgagttccgc ccccagcaac
     3001 aggagatggc ggtggcggta gcgcgcgcgc tggacgcggg cgagcacctg gccgtcgagg
     3061 ccggcaccgg ggtggggaaa agttttgcgt acttggtgcc ggccatcctg tatgcggtgg
     3121 cgaaccggaa gaaggcggtg attgccacgc ataccatcaa cctgcaggag caactgacgc
     3181 agaaagacct gcccgtgctg gcgcaagtcc tgccggtgaa gttcagcttc gccatgctca
     3241 agggccgggc caactacctg tgcacccgcc gcctccggaa ggcgctccag caggcgccca
     3301 acctctttac cacgtcggaa acgcaggagc tgcagcgcat cgccgaatgg gcccggacca
     3361 ccgaggacgg cagccgttcg gatttcgacg tggagccgga cctgaaggtg tgggaccagg
     3421 tctgctccga gcgcggcctg tgcgcgccca agctgtgcgg ctacccgtcc gatttcgccc
     3481 gggagcacgg cgtttgcttt ttccagcggg cgcggcagcg catcctttcg gcggatgtgg
     3541 tggtgctgaa tcacaccctg ctgttcaccc tgctgggcgg ccaggaagag agcgcggacg
     3601 gcggcatcct gttccgcaat gactttgtca ttttcgacga ggcccaccaa atggagcacg
     3661 aggcctcgaa gcatatcggg ctgaccgttt ccagcggcca ggtgcgctac gcgttgcatc
     3721 gcctctggaa tccccgcacc gagaaggggc tcctggccac cctgcgcaag ggcgcggcgg
     3781 tgaagctggt cgccgagttg ctggcggcgg cggacggctt ctttgccagc gtggagcacg
     3841 cctgcgacga actgctgccc gcgtcccgga aacgcatcgg cggcggcggc gacgcttcgg
     3901 gcgcgcggcg gcgcgagtgg accgagctgc gcatccgccg tcccgagctg gtgccggaca
     3961 aggtcacgct gcctctccag cgcctgcgcg aggcggtggc cgacctgatc aagctgagcg
     4021 aggacagcga gatggggctg gaactgatgg aaggcaaccg gcgcctggcg gaactgcggg
     4081 agggaattgc cgccttcctc agccagagcg ccgcggacca cgtttattgg gtcgagcgaa
     4141 ccggccgggc ccagaagacg ctggcgctgc atgcggcgcc ggttgacgtg gccgattacc
     4201 tgcggcagcg gctgttcctg ccgggcacct cggtcgtgat gaccagcgcc acgctctcgg
     4261 tctccggccg ggaggcgccg gcggtttccg acgacgcccc cccgcgcggc gcgcggtcgg
     4321 ccggcggcgc gccgccggga gcggggctcg attacttcgt ccatcgcgtg ggcgccgaag
     4381 ccgccgtccg gcttcaggtg ggcaccccgt ttgacttcga gcgccagatg aagctgtgcg
     4441 tggtgcggca aatgcccgac ccgcggacgg aagactaccg ggacgcgttg atccactgga
     4501 ttgagcattt cgtgaagcag acgcacggca aggcctttgt cctctttacg aacttcaagc
     4561 tgatgcagga agtcggcgaa ttgatgcagc cgttcttcgc gcggctgggc ctggcctgtt
     4621 tcgtgcaggg caccggcatc ccgcgctcgc ttatgctgga gaagttcaag gaggatgtgg
     4681 attccgtgct gtttggcacg gacagtttct ggcagggggt ggacgtgccc ggcgcggccc
     4741 tgtccaacgt catcatcacc cggctgccgt ttgccgtgcc ggaccatccc ctgatcgagg
     4801 cccgcatcga ggccatcgaa gcgcgcgggg gcaattcatt ccgcgacttc tccctcccgg
     4861 aagccatcct gaagttccgg cagggggtcg ggcgcctcat ccgcaccaaa accgaccagg
     4921 gcatcatcgt ggtgctcgac aaccggctgc tggcgaagca atacggccag gcctttattc
     4981 aggcgctgcc ccggtgcccg gtggaagtgg tgtagcttga caaccgcggg ggcaagcctg
     5041 ttaatgccgg cggaaagccg gaagcattgc tgtgacctcg gataaataca gtccgcccaa
     5101 gtatttccag atttgccgcc gcatcgtggg cgccattcag cgcggcgaac tgccgcccgg
     5161 cgcgccggtg gcctccgaga acgaaatcat cgagcgctac ggggtcagca acaccaccgc
     5221 gcgcaaggtg ctgcatgaac tcgaacagga aggctgggtc acgcgcgtaa agggcaaggg
     5281 cacgttcgtg cgcgatttcc ccgtgctgcg gtccatcaac cgcatcttcg gcttcacgcg
     5341 aaacatgatc gaggccgggc gcaagccggc aacccggctc ctgggctttc acctgcgcga
     5401 gggcgaccat tcgcaaacga tcaatggccg gctctacacc ctcaaagggc cgttctgcga
     5461 gattgagcgg ctgcgcctgg ccgacggcat cccgatgatg aaggagaccc gctacctctc
     5521 gctgagcctc tgcccgaaca tccaccggcg caacctcgaa caatccctgt accagatttt
     5581 cgacaagcac tacggcatcc ggctgaccga gatcaaccag atgctcagcg cggtcgttct
     5641 ggagggggag tggctcccgg tctttcaggt ggatcgcccc atccccgcgt tccgggtcga
     5701 gggagcttcc ttctgcggca aggacctgat tgtcgagatg gaaaactcgg tttaccgggg
     5761 cgaccggtac cgctttgcgg ccaaggcggt gcgctgaggg cggacgatga tcggagacct
     5821 ttgggcggcc agcgcggggg atctcgccca ccacgccggg aagctggcgc tgttggggct
     5881 ggatttgctg ctcgccgggg tggcctccgg gcacgccatt ctctataagc gcgactcgcg
     5941 ctcggccatc gcctgggtcg gcttcatctg gctggtgccg ctgggcggcg cggcgcttta
     6001 cttcatcctg ggcatcaacc gcattcgccg ccaggcggtg ctgctgcggc gcggcctgga
     6061 gcgctaccgc gcccatgcgg ccctgcccgc ctgcccgccg gacgaactgc cccaccacct
     6121 gcccggccac accggccacc tcaacatgct ggcgcgcgtg gtcaacgggg tggtggcgcg
     6181 cccgttgctc ccgggcaacc ggatcgagcc gctgctcgac ggcgacgaag cctacccggc
     6241 catgctcgaa gccatccggc aggcgcgccg gacggtggcg ttttacacct acatcttcga
     6301 ccgcgatgcc gccgggctgg cgtttgcgcg ggcgctgggc gaagccaccc gccgcggcgt
     6361 tgaagtgcgc gtcctgattg acgcggcggg cacccgctac tcctggccca ccatcctgcg
     6421 cgcgctgcgc cgcgaaggcg tgcgcttcgc gcgcttcctg ccctccctcg cgctctggca
     6481 cctgatggcg gtgaacctgc gcacgcaccg gaaaatcctg gtggccgacg ggcggctggg
     6541 attcaccggc ggcatgaaca tccgcgcggg gcattgcctg aagggcttcg gcggcggcgc
     6601 cggggatggc cccgcccgcc cgccgcgcca cccggtgcgg gacatccatt tccgcgtgcg
     6661 cgggccggtg gttgcccagt tgcaggaggt cttcgtggac gactggctgt ttacgaccgg
     6721 cgaattactg cgcggggagg actggtttcc ccacccggag ccggccgggc cggtgctggc
     6781 gcgcggcgtg ccggacggcc cggacgaaga ttttgaaaag ctgcgctgga cgctgctggg
     6841 cgcgctggcc attgcccgct acgcggtgcg gatcgtcacg ccctacttcc tgcccgatcc
     6901 ggcgctgatc tcggcgctga atgtggccgc caagcgcggg gtgcgcgtgg atatcatcct
     6961 gccggcgaag aacaacctgc cgttcgtcgg ctgggcctcg cgcgcgatgt ggtggcaggt
     7021 gctcgaacac ggctgccaca tctggctcac cccgcccccg ttcgatcata cgaaactcat
     7081 gctggtggat ggctgctggg ttctgctggg ctcggccaac tgggacccgc gcagcctgcg
     7141 cctcaacttt gaatacaacc tcgaatgtta tgacgcggag ctggcgcggc ggctggagga
     7201 aatcgtggcg gcccgcattg ctaccgcccg cccggtgacg ctcgccgacg tggacgcccg
     7261 ctcgctgccc atccgcctgc gcgacggcct cgcccgcctg ttcaccccct acctctagct
     7321 ccgcccgccc cggccgcggc gcgcgcgggt taaagcttgc atgaagcggc gcggcaggaa
     7381 ataactttgg tgtgaaagtc ccattgctgg atctcaaggc gcagtatgcc gccatcaagc
     7441 ccgacctgga cgcggtcgtg gccgaggtga tggtctccca gcagttcatt ctcgggccgc
     7501 aggtcgaggc gtgcgaacgg gccatcgccc aatactcccg ctgcgcgttt ggcgtgggcg
     7561 tctcctccgg caccgacgcc ctgattatct gcctcatggc cgagggcatc ggcccgggcg
     7621 acgaagtcat cacctcgccc tacacgttct ttgccaccgc cggctccatt gcccgcgtgg
     7681 gcgcccggcc ggtctttgtg gatatcaatc ccgccaccta caacctggac gtggcgcagg
     7741 tcgaagcccg gatcaccccc cgcacgcgcg ccatcatccc cgtgcatctc tacggccaga
     7801 tggccgatat ggaagccctg atgccgatcg cgcaaaagca ccagctcgtg gtcatcgaag
     7861 acgccgccca ggccatcggc gccgagcatc acggacgccg cgccggctcg atcggccact
     7921 acggctgctt ttcctttttc ccctccaaaa acctcggggc ggccggcgac gccggcatgg
     7981 tcgtcaccca ggacgcggcg cgggccggga agctggcctg cctgcgcatg cacggctccc
     8041 aggccaagta ctaccaccag gtcatcggcg gcaatttccg cctcgatgcc ctccaggccg
     8101 cggtggtgcg ggccaaactg cgctacctcg atgcctggac agccgcccgc cagcgcaatg
     8161 ccgagcgcta ccaccggctg ttcacggaag ccggcctgcc gctggccggc agcggcgaat
     8221 accgcgcccg ggccggagat gcctccgttc ttttcctgcc cgacgtcgcg accgaccgcc
     8281 acatcttcaa ccagtacgtc attcgcacgc cgcgccggga cgccttgcgg gcggccctgc
     8341 aggcccggga tattggcact gaaatctatt acccggtgcc gctgcacctg caggaatgtt
     8401 tcgcttacct gggcggccgg ccgggcaccc tgcccgaaag cgagaaagcc gcgcgggaaa
     8461 ccctggccct gcccatttac ccggaactct ccgaggccca ggcccggcag gtcgttcaat
     8521 ccatcaccga attcatgtcc gcttcctgag ttatgaaccc gattccctcc cccgaaattc
     8581 ccccactccg cggcgagcgc atcctgcgcg acccggccca gaacaaagac gccgccttca
     8641 ccgccgccga gcgccggcaa ctgggcctgg agggcctgct gcccccggcc gtgctcaccc
     8701 tggcccagca ggtcgaactg gagctggaac acttccacca gaagaccgac ccgctcgaac
     8761 aatacatcgg cctgatggcc ctgctcgacc gcaacgaagt cctcttttac cggctgctga
     8821 tcgagaacct cgaccgcctt gcgcccatca tctacacccc caccgtcggg caggcctgcc
     8881 agcgcttcag ccacatcttc cgccgcccgc gcggcctgtt cctttgcccc gccgaccgcg
     8941 gccagatggc cgcgcgcctc ggccagctcc gccaccgcga catccgcctc atcgtcgtca
     9001 cggataacga acgcatcctg ggcctgggcg accagggcgc cggcggcatg ggcatttcca
     9061 tcggcaaact ggttctctac tccgccggcg cgggcatcca tccgcggctg accctgccga
     9121 tcagcctcga tgtcggcacc gacaacgccg ccctgctcga agacccgctc tactgcggct
     9181 accgcgcgcg gcgcctccgc ggccccgaat acgacgcctt cgtcgaggaa ttcgtccagg
     9241 ccgtcaagca cgtcttcccc cgcgccctcc tgcaatggga agacttcaaa aaggccaacg
     9301 ccttccgcct cctggaccgc tacgcggcgc gcctccccag cttcaacgac gacatccagg
     9361 gcaccgccgc cgttgccctg gccggcattt tcaccgggct gcgcctcacc ggcaagaccc
     9421 tgcgcgagca gcgcttcctc atggccggcg ccggcgcggc gggcgtcggc atcggccgcc
     9481 tggtccgcac ggccatgctc gaagccggcc tcaccgaggc ggaaatccgc ccgcgccaaa
     9541 tcttcctcga ttccggcggc ctggtctggg aaggccgcgc cgatctcgag gaccacaaac
     9601 gcgcggccgc cctgcggaaa gacgaactgg cgttgctggg cctgtccacc ccgccccccg
     9661 ccgcgctggc ggaaatcgtc cgcgccgtcc gccccaccat cctgatcggc accaccggcc
     9721 agcccggcga tttcacccct gccgtcctgc gcgccatggc cgaccactgc gagcggcccc
     9781 tcatcttccc gatgagcaat cccaccagca aaaccgaatg cacccccgcc gaagccttgc
     9841 agcacaccga cggccgcgcc ctggtggcca ccggctcccc cttcgacccg gtcgtcttca
     9901 acgggcgcaa atacgtcatc ggccagtgca ataacgcctt cgtcttcccc ggcgtcggcc
     9961 tgggcgcgct catcgccgaa gccagccgcg tcaccgattc catgttcctg gccgcggccc
    10021 aggcgctggc cgacttcacc gtccagcatc ccgccggcga aaactgcctc taccccagcc
    10081 tccagcaact gcgcgccgtc agccgcctca tcgccctgaa agtcgctcag accgcccgcc
    10141 acgaggcgct cggccggaac ctggaggatg ccgccctgca agcggccatt gatcagtttt
    10201 tctggcaccc cgattacccc cccgccagtt aaacggcccg ccccgggcct gacccggacc
    10261 gggcgttcga aatatccgcg cgccggcccg aaaccgggaa gcggcgccgc gcccgctcga
    10321 tcccgcccgc ggcctcagga tttggagtgc tttttgagcg tcgaatccag cagcgacagc
    10381 agctccgctt tcgagatcag gcccacgctt tgtttgacca tcttcccctc cacaaagatc
    10441 agcaacgtcg ggatgccttg aattccaaac ttattcgcca gcttgtgctc ctggtcaacg
    10501 ttgactttgc cgaagataat atccggccgt tcctgggcgg cggcggcgaa gatggggtcc
    10561 atcttccggc agggaccgca ccagggcgcc cagaagtcca ggacaatggg tttggtggac
    10621 tccttgagga gttgatcgaa cgaagcgccg gtcaccacca cccgggcatc gggcttgtcc
    10681 gggtcggggg ccgtcgtttc cgcgtcggct tttttcattt tggtgcatgc cggcagcacg
    10741 gccagcgcca gcaggagcgc gccgccaagg ataagggaag agcggtatcg gagtaatttc
    10801 atgccgccaa tgtacgcgcg gcccgcccat tgacaagccg gtttcgccac ccgccgccgc
    10861 ccccaacggc ccgccggagc cgcccgcccg cccgggcttc cttcgcgtat ttcttgaccc
    10921 gcatcaaagc ccccgccgcc gcgccgggtt agcttccggg catgaacaag tctatcgaga
    10981 tcatgatgcg ggagcataag ctgattgtgg gggtgctggc ttcgttgcag gcgctggccg
    11041 ataaactggc ggcgggggaa acggtcgccc gcgaggacgt ggccgacttt ggccggttct
    11101 ttcgcgattt cgccgaccaa tgccaccacg gcaaggagga agaccggctt tttgtccaga
    11161 tggtcgaagc gggattgccg cgggaccgcg gccccatcgc cgtgatgctg gcggagcatg
    11221 acgcgggacg gcaggaagtg cgcgggctga tcgaagtcgg cgccggcgcc ggcccgctga
    11281 gcgagagcga gcgggcccgg gtggtggatt acgcccgccg gttcgtgccc ctgctctcga
    11341 gccacatcca caaagaggat catatcctgt atcccatggc ccaggaaacg attccggcgc
    11401 cggcgtttgc ggcgctggac caggcctgcg aagacttcga ccgccaggtc cggcagcagt
    11461 tggacgtgcc cggcctggaa gacctcgccg cccgcctgat ggagcgttac ccggcctgcc
    11521 tggggcagtt atccgacgac caccgctgcg gcgcctgctc cgcggcgggc tgagcgccgg
    11581 ggtcaatcca gcggctgcag cgtgacccgg cggaactgaa tcacgccgcc ctcggactgc
    11641 aggccgatca gcccggcgcc gcgatgcacg ccggacacgt cgttgaccag tttgccgttg
    11701 agccagacgc ggtagcgctc gccccgcgcc aggatttcca cccggttcca ttcgcccgga
    11761 ggattttccg cctccagcag cttgctgatg ccggcaatat ccccgccctc ggggtgctgc
    11821 cggatctcga agaagcgggg ctggccgcgc gcaatgggct tgccttgcag gccaaacaca
    11881 tcgccggcgt tgccatattt taattgcact tcgaccgcgc aggggagcgg cgagaacggc
    11941 gcgttcaggc gggtaaagat gccgctattg cccggcgtgt ggcccggcgg ccagcgatac
    12001 tccaccgcca ggcggaaatt gcccgccgcc ggctcccggt acagaaatcc caggggcgtg
    12061 ccctggcagg tcaggatgcc atcctgcacg ctccagacct ggtccatcgg aaccgccgga
    12121 tccgccagca cgctcttcca gtcggccagg tccttgccat tgaataattc cagcccggtg
    12181 gggcggccgg cgggagtcga gcacgacacc gctccccaca aaatcattcc tccgcagacc
    12241 cacaggcgcc caagaccgtt gattcgagtt ttcatagcca ggctcgtccc ggccagtatg
    12301 cctgaaaggc cgcgccggca aagctcaatc gccaggccgg ggaggacggc ggcgggaacc
    12361 ccgcccggcc ccgaccgccg ccgcgcgccc cgccgcgggc ggcgtctttc cgcttgcgcg
    12421 cggcggcgga aacggctatc cccgcgcgca tggagcctca cgaagccctt gtggcgctga
    12481 acctgatcga gcgcgtcggc ccggtgcgcg tgcggcaact gctcgaacag ttcggcgagg
    12541 ctcccgccat cctgcgcgcc tcccgccggc aattgctcca ggcccggggc atcggcgagg
    12601 acatcgccga cgccatcgtc aactgggaga aaaccaccga cctggccgcc gagcttaagc
    12661 gcatcgccgc gttcggctgc cgcattgtga cccaggccga cgccgaatac ccggagctgc
    12721 tgcgccaaat ctacgacccg ccggtggtgc tctacgtcaa gggccggctc catgcccagg
    12781 acaagaactc cgtggcggtg gtgggctcgc gcctgaccac gccgtacggc ctcgaaaccg
    12841 cccgcaaact ggcccgccag ctcgccgccc tgggcgtgac cgtcgtcagc ggcggcgcgc
    12901 gcgggattga caccgccgcc caccagggcg cgctgagcgg ccagggccgg accctcgccg
    12961 tcctcggcac gggcatcaac ctcgtctcgc cggcggaaaa cgcaaagctg ttcgggcaga
    13021 tcgccgacca cggcgcgctg ctcacccaat tccccttcaa ccgccccgcc gaccggcaat
    13081 ccttccccat ccgcaaccgc ctcgtggccg gcatgaccct gggcaccgtc gtcgtggaag
    13141 ccaacctcac cagcggcgcg ctcatcaccg ccaatttcgc cgccgactac ggccgccagg
    13201 tcttcgccgt ccccgggcgc attgactcgc cccgcagcaa aggctgccat gacctcatca
    13261 agaaaggggc caaactctgc gagggcgtcg aggatatcct cagcgaattc gaatacctct
    13321 tcccgcccgc cagcccgccc tcctcgcccg ccgccgccgg cccccggccc gccgtgccct
    13381 tgaacgaaaa cgagcaaaaa gtccttggcc tgctgggccc ggaagaaagc cacattgatg
    13441 aagtcatccg ccgcagcggc ctgcccagct ccgcggtttc cgttgccctg ctgagcctgg
    13501 agatgaagcg gctcatccgg caactgcccg gcaagctctt tgcccggaat ggctgatgcc
    13561 ccaaccgccg ccccggggcg gcttcccgaa agaccgaaaa cgccccaacc cggacgcgcc
    13621 tgttccgaaa tgagacagcc cccgcccgcg cggccaaccc ggccgccgaa ggcgttggcg
    13681 gagcggattc cccaccggcc cgttcagccc gcaaaccgcg ctttgcgccc cgcgtccatt
    13741 tcaccctgaa tcaggccgtt ttcgcgggtt ctgccgtcaa cccggccgga acccttcggc
    13801 tcgccgccgc cgccggttca agccgccgct ccggggccgg cgtttacgag ggccacccgc
    13861 gccggctggc acaatctctg cggcaaaaaa cgcacaagca caaaccgcta acgaaagccc
    13921 atcacctgtg aagtttgcca tcgagatcgg ggacaccgaa aagcatcgcc tggaatacaa
    13981 ctttaatcag cttctcggga gcctggtcat caaggtcaat gaacggccca tcaagaaatc
    14041 ggtccggctg atcaacgagc cggtgctgga ggtgcatgcg tttatcgtgg gcgaccggga
    14101 aaagtccagc gtgcgcatcg aaaaagaacg gatgccgctg ctgggaggcc gaaaccgcat
    14161 ctacgtcaac gaccgcctcg tcagagtctt tagcggcatc tgaccccgcg aacgccggaa
    14221 acccggccgg gcggcggccg gctcgccccg gaaacggcag ccccggaggc agggccgaac
    14281 tccattccca aaatcaacgc cattgacact ggcggcggcg tggaagccgc agagccagac
    14341 cctcggttgg gatagggcct cgccaacgcc gccccccctg ccgaacccac aggtcagaac
    14401 cgcgaaaacg gcaggccctc ccccggcccg gcaatcccag cccccaaaat cgccattcgc
    14461 gtcaaaatcc ctccctcccc cctcctttcg agccgtgtac aaggggtgta caagggatgc
    14521 tcaccgggag ttcatgggat tttagctggt acatccgatg agca
//
LOCUS       DAOPQG010000089        14533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4869_length_14533_cov_14.316480, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000089 DAOPQG010000000
VERSION     DAOPQG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4869_length_14533_cov_14.316480"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            681..1160
                     /locus_tag="PKI07_10270"
     CDS             681..1160
                     /locus_tag="PKI07_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOC51475.1"
                     /translation="MPIPVGSKAPDFTLKSKQASGLVDVKLSANFGRKNTVLLFVPAA
                     FTGVCTQELCDITNGLNAYASLNADVIAISVDTPFAQEAWAQKEKIGLTLASDLNKEV
                     TKKYDVLFPMLLGIGDTSARAAFVIDKHGVVQYSEQTPTPKDLPNFDRIKETLARLK"
     gene            1300..2865
                     /locus_tag="PKI07_10275"
     CDS             1300..2865
                     /locus_tag="PKI07_10275"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOC51476.1"
                     /translation="MIKLNTVAAAALLASLAFCPAVYGEGLDHLLRVQDYTARRVSSA
                     DPSGGNLDMRRVEAGKSLTLAELPGPGIVTHIWFTHLYPSRSGLRKLVLRIYFDDLPE
                     PCVEAPLGDFFGLGHAQTYAYASQPLAVGTHGGLNCYWPMPFAKTARLVVSNEGAQDC
                     SALYFQIDYRTLAKPLPDDLHFFAAYRQEFPPVKGKPYLILETDGGRGHFAGCNLSVE
                     QRDESWWGEGDIAIHVDGEARPSLAGTGSEDDFGGAWCYSHEFSYPQFGAPLRARFNA
                     QGVLEHCTPDLKDKALTQWRWPEAWKPGDLWNVYRYHVADPVPFRKSVRVEIEHGWQG
                     NERADWYSSVAYWYQTGRPAVRAALPAVKDRLPYYLRARDQGGGRWEAENLVDDAVAT
                     GGTVQEAGMEFWGDLFSERYALQWEAGKTNDILTVPFTVSQAGRHRVTVRLARGEAGG
                     AFTLQVDDLKPGEPVDLFQAGAIPGLFETLAGEGNLAAGAHALKFTCLTPDSRAKGKR
                     LLLDKLQVSPAGE"
     gene            complement(2945..3799)
                     /gene="mutM"
                     /locus_tag="PKI07_10280"
     CDS             complement(2945..3799)
                     /gene="mutM"
                     /locus_tag="PKI07_10280"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOC51477.1"
                     /translation="MPELPEVEVLVRHLAPLLKGRRIDAVQVRLAKVVRPDSPRRFAQ
                     ALRGARFRAVSRRGKYLLFHLRPAGQGGALTLTGHLGMTGRMYLLPAKTPLPRHAAVV
                     LGLGRQNFIFEDSRRFGRLTLAAAPRRNLGPEPLESAFALRPFVAALRQSRQAIKVKL
                     LDQSLLAGVGNIYASEALFRAGIAPALPARRLSESQVRRLRLAIRAVLRQAIAGGSTV
                     PLNYTGQGASDRLFYFGRAPGAPDYYEERLQVYDRAGQPCPRCKTPIRRRVQAARSTY
                     YCPRCQRA"
     gene            complement(3847..4491)
                     /locus_tag="PKI07_10285"
     CDS             complement(3847..4491)
                     /locus_tag="PKI07_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOC51478.1"
                     /translation="MIFRSREDAGLKLANLLQTQAVQADLVLGLPRGGVIVAAQVAQA
                     LRRPLGVITVRKIGHPWNREFAVGALAEGDITLLDERAVGPNPRVRAELAAVLEEEKE
                     RLRACQAQFHPEGAPVLAGKAVLLVDDGLATGATTEAAVLSAKARKAGFIVVAAPVAS
                     SGAVNRLAQVADEVRVLHVAPDFAAVGQYYESFAQNTDEEVLAVLRAATQAHPR"
     gene            complement(4566..5291)
                     /gene="pyrH"
                     /locus_tag="PKI07_10290"
     CDS             complement(4566..5291)
                     /gene="pyrH"
                     /locus_tag="PKI07_10290"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOC51479.1"
                     /translation="MKQAKRPKYKRILLKLSGEALGGPTGLNINPQAVRSMAGQIRAV
                     RKLKVQIVIVVGGGNIFRGLSGSKRGMDRTTGDYMGMLATIINALALQDALEKLEVPT
                     RVLSAISISQVAEPFIRRRAVRHLEKGRVVIFAGGTGNPYFSTDTTAALRANEIGADV
                     VLKATKVDGIYDDDPQKNPKAKRYSQICYSDALRQRLKVMDSTAFSLCMDNKMPIIVF
                     NFSQPNNLKRIILGEQIGTLVAD"
     gene            complement(5409..8483)
                     /locus_tag="PKI07_10295"
     CDS             complement(5409..8483)
                     /locus_tag="PKI07_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC51480.1"
                     /translation="MTPSPRLIRQQMRAGILFLPRLMILAAMAGAYLPSPGVVQAATP
                     PITRPNIIVLLTDDMGCGDLGCYGGDLVPTPNLDRMAREGIRFTQYYAASPICSPSRT
                     GLLTGMHPARWRITSFLQTRQGNHACEQADFLDPSAPSLARALKAAGYATGHFGKWHM
                     GGGRDVTNAPPFPAYGFDEHASTYESPDPHPDLTATNWIWSAQDKVQRWDRTAYFVDR
                     TLDFLNRHRGQPCYVNLWPDDVHTPWVPNRERLSEFPNGAQTERNFIGVMAEYDRQIG
                     RLLAGLKELGLDEKTLLIFTSDNGPLPTFRGRRTAGLRGSKLSLYEGGIRMPFLVRWP
                     GQVPAGRTDDQTVLSAWDLFPSLCALAGAPLPAGAALDGENLSPALLGRPVAARAKAL
                     FWEYGRNEHAFAYPKGTNRSQNVAIREGDWKLLLNADGRQRELYNVATDPGETTNQAA
                     AHPALADRLGAKALEWRKSLPRPASASAQSSQPDIVLIMSDDMGFSDLGCYGSEIRTP
                     NLDALAKEGLRFTQFYNTARCCPTRASLLSGLYPHQAGMGHMTGHGSGREDGYAGDLN
                     RRCVTIAEALRPAGYRTYLSGKWHVANIIAPTGPKDTWPLQRGFDRFYGTITGGGSFY
                     DPTTLCRGNTYITPDNDPEYRPTRFYYTDAISDNAITFIRDHARDHSAQPFFLYVAYT
                     AAHWPMHAPAEEIAKYRGLYDGGYGPIRAARFARLKELGLIDPAWQLPPPAEDWDAVT
                     NRAWESRCMEVYAAMVDRMDQGIGRIVAELKRQNRFDNTLLLFLQDNGGCAEPMGRKS
                     NADEIKTMTCQPMAPDELQKKIWPPMQTRDGRPVRTGPEVMPGPEDTYVAYGRGWANV
                     SNTPFREYKHWVHEGGIATPLIVHWPRGIASSRRNQLVTQPAHLVDLMATCVDVAGAV
                     YPAEKDGQKIQPLEGVSLRPALDGKPLHRAQPLCWEHESNRAIRDGQWKLVAKAGQPW
                     ELYDLTADRTEMNDLAGRYPDKVKELSARWEAWAARANVLPLGSWRGKRAAK"
     gene            complement(8872..13773)
                     /locus_tag="PKI07_10300"
     CDS             complement(8872..13773)
                     /locus_tag="PKI07_10300"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC51481.1"
                     /translation="MRLLTRAIRVAPRLGALLIACQLTHSAYGVIRDGGIDPANLGKG
                     DWIYQLHHAVAQCNGNVPSVTDVPSLMTYLKNQGMQYIIVKAGTGGALFSVPGYSPQF
                     SSSLVDAAHNAGLKIFGYTRSDGLDIPGESAVADWVFNQGADGFVFDAEIEWESHNLP
                     NNTAKAIQLCSMVRSNWPNKFLAHAPFPYISVHSTFPYKEFGYYCDAVMPQVYFIQIG
                     VTPTQCVNKMNTDFRNWQNGLYGQWRNSIKPIVPLGQGWHGEGTITPALITEFVDAVK
                     NVANPATAGGFKGVNYWVCELHPAAIWPSIATNEITLWTNAPVVANVSADNINSTSAT
                     VTWTTDQKSDSVVEYGLDTNYGTSVTNSSPIYVHSVTLSGLSPYTTYYYRVKSKNANN
                     QTGVSGVNVFATTAVTAPDVIVESYLPNGALNSNPPYTDAGFVAFSTCKSTAPGLTAP
                     AKVQYATGGSGTPSVTLRPTLPIAGGAYKVYVTHCATSCSADLVASITQSGCSGLPAT
                     TPVFQSSYANSWGYVGQMTLSPGETMPTITFTKSGGTLSASSRMYSDGYKFVYEPPPP
                     VGPSIVTQPAATQTNLQGSAASFSVVASGSPPLHYQWRLNGTNIPNAILYTYTRNNVQ
                     PADAGTYTVVITNTAGSVTSSPSILVVALPAKITTQPVDTTVGIGMTALFSVTASGTA
                     PLSYQWQFNGTNINGATAAGLAIFNAQATAAGEYAVMVSNPYDTVISSIATLTVMDPF
                     IVTQPQNQSVAAGATATFSVSAVGSPTLSYRWRKEGVDLFDGGRISGAATPTLTVANA
                     QAMDMGNYSVVVSNSNSQIVSSNATLLGQFPPVIVSQPVSQKVVAGSSAQLTAAAVGP
                     APLVYQWRKGGINLADGGKISGAATATLTISDMQIDDCGDYSLFVENAYGNATSSNAL
                     VGLAPLAAWGRNNYTQTDIPAGLNTVSAIAAGFYHCLALRTDGLVAAWGAGMTNAGAN
                     PHLGQAVVPDSLSNVVGIAAGYFHSLALRSDGSIAAWGAGTNNSGASPHYGQAMIPGG
                     LGSVLAVAAGGYHSLALKADGTVAAWGAGTTASGNPHYGQAMIPSGLNNVISIAAGGY
                     HSLALKADGTVVAWGAGTTTSGNPHYGQAIVPNGLTNVAMIAAGGYHNLALKADGTVV
                     AWGDNSYGQTNVPAGLTNAVAISAGRYCSLALKADGTVVAWGDNSYGQTSTPNSVANV
                     IGISSSGYHNLALVNDGRPYITVPPRGQVAAANSNVSLTVMAAGAPPLSYQWQRNGTN
                     IDGATTSALHLAGVQSSDGGNYAVIVSNALGGATSPIAALAVLAPPTILEHPQSLTVN
                     EGGNATFTVTAAGTAPLSYQWQFNNVSLAGATDSHYTRSPVQAAHAGIYAVVVSNALG
                     SVTSSNATLAISVPPGIIAQPQSATVIAGTNITFTVTATGTAPLSYQWIFDGANIPGA
                     TASSYTRINVQPTDAGAYAAIVTNVAGSVTSSNALLTVLVPPVIITQPQSIAVAAGSN
                     ATFTVIATGTVPLEYQWRFNGTNLAAATDSAYTCWNVQAENAGSYSVVVSNLAGTVAS
                     SEAELTLIPSAPLQIDWIRLTPGGQIQLQVSGGPGDYTLKVTTNLVNWEALTNLTTAG
                     PSFQFLDAETNLSQRYYRVQRLP"
     gene            14287..>14533
                     /locus_tag="PKI07_10305"
     CDS             14287..>14533
                     /locus_tag="PKI07_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51482.1"
                     /translation="MKILSAPRTGSLGAETYYQSPFGVCARRRTVPRDRPSNRKAAAR
                     SYFGISSREWGLKLSEAQRERWNAAALHVPSRPWMGQY"
BASE COUNT         2600 a         4176 c         4971 g         2786 t
ORIGIN      
        1 ttacagattg cgaggtgggg gcgcaatacc cggagaaacg ccaaccatca ggaggccgag
       61 gtcaggtctg ctttcgtcgt ggcaaaaccg cttaggaacc cctcctttcc gggtcaactc
      121 ccggttttgg gatattccag tgaatatccg ccccaatagg ctcgattcgc ttccggccag
      181 cgcaaccccc ggttttcctt aaattacctc attcccaggc tcatttaggg ctcccaatgc
      241 gccgtgtaca aggggtgtac accggatgct caacgggtgt accagctaaa accctatgtg
      301 catccggtga gcatccggtg tacacccgtt gtacacgaca tattggggcg ggaaaatgac
      361 gggaaatgga gggaattgga gagctggcag gggaaacgct ggttggggag ggttgccggt
      421 ttggttggtt ggggaaaggg cttgttttcg cctttggagg ggggccgtac gagggtgatt
      481 acggccatta gcgccagggc tgaagtgtgc caaaatggca cagttcgccg gagggccggg
      541 aatggagcat aaaagtcggc gaagagggta gccaggcgcg ctttagccgc gcttttggcc
      601 gtcttgccgt ttgacaggcg gcccaacccg gcttacctgt ttatccaata gataaactca
      661 atcattaaca aagtataaat atgccaatac cagtaggttc caaagcacct gatttcacgc
      721 tcaagtccaa gcaagcctcc ggcctggtgg atgttaagct cagtgccaac ttcggccgga
      781 agaacaccgt gctgctcttc gttcccgccg cttttaccgg cgtttgcacg caggaactgt
      841 gcgacatcac caacggactc aatgcctatg ccagcctcaa cgcggacgtc atcgccatta
      901 gcgtggacac gcccttcgcg caggaggcct gggcgcaaaa ggagaaaatc gggcttaccc
      961 tggccagcga cctgaacaag gaagtcacga agaaatacga cgtcctgttt cccatgctgc
     1021 tcgggatagg cgacacctcc gcgcgcgcgg cctttgttat tgataagcac ggcgtggtgc
     1081 aatacagcga gcagaccccg acgccgaagg acctgcccaa cttcgatcgg atcaaagaaa
     1141 cgctggcccg gctgaagtag gcccgccagg gcgggagcgc ggacgccccc ggcaatccag
     1201 gctttcggag agcgcaatct ttgtaaggtg atccccgggc gcgacccggc ggatcaggca
     1261 aggcttgcgt acgggcgggt tttggcgcca tgctggcgca tgattaaact caatacagta
     1321 gccgcggcgg ctttgctggc gtcgctggcg ttttgcccgg cggtttatgg tgagggcctg
     1381 gatcatttgc tgcgggtgca ggattacacg gcgcggcggg tttcgagcgc ggaccccagc
     1441 ggcggaaacc tggacatgcg ccgggtggag gcgggcaagt cgctgacgct ggcggagttg
     1501 ccggggccgg ggatcgtgac ccatatctgg ttcacgcatt tatacccgtc gcggagcggg
     1561 ctgcggaaac tggtgctgcg catctacttt gacgatttgc ccgagccgtg cgtggaggcg
     1621 ccgctgggcg atttctttgg gctggggcac gcgcaaactt atgcctacgc cagccagccg
     1681 ctggcggtgg gcacgcatgg cgggcttaac tgttactggc ccatgccgtt cgcgaagacg
     1741 gcgcggctgg tggtgtccaa cgagggggcc caggattgca gcgcgcttta ctttcagatt
     1801 gattaccgga cgctggcgaa gccgctgccg gacgatttgc atttctttgc cgcttaccgg
     1861 caggagttcc cgccggtgaa aggcaagcct tatctgattc tggaaaccga tggcgggcgg
     1921 ggccatttcg cggggtgcaa cctgtcggtt gagcagcggg acgaaagctg gtggggcgag
     1981 ggcgacatcg ccatccatgt ggacggggag gcccggcctt cgctggccgg cacgggttcg
     2041 gaggacgatt ttggcggggc ctggtgctat agccacgagt tttcgtatcc gcagtttggc
     2101 gcgcctttgc gggcgcggtt caacgcgcaa ggcgtgctgg agcattgcac gcccgatttg
     2161 aaggacaagg ccctcaccca gtggcgctgg cccgaggcct ggaaaccggg cgacctgtgg
     2221 aacgtgtatc gttatcacgt tgccgacccg gtgccgttcc gcaagtcggt gcgggtggag
     2281 atcgagcatg gctggcaggg gaacgagcgg gcggattggt attccagcgt ggcgtattgg
     2341 taccagacgg ggcggccggc ggtccgtgcg gcgttacccg cggtcaaaga ccggttgccg
     2401 tactatttgc gggcgcgcga ccagggcggg ggccggtggg aggccgagaa cctggtggac
     2461 gacgcggttg ccacgggcgg caccgttcag gaggcgggca tggaattctg gggcgacctg
     2521 tttagcgagc gctatgcgct gcaatgggaa gcgggaaaga ccaacgacat cctcaccgtg
     2581 ccgttcacgg tttcccaggc cggacggcac cgggtcacgg ttcggctggc gcgcggggag
     2641 gccggcgggg ccttcacgct gcaagtggat gacctgaagc ccggcgagcc tgtggacttg
     2701 ttccaggcgg gggcgattcc cgggctgttc gagacgctgg ctggcgaggg caacctggcg
     2761 gccggggcgc atgcgctgaa gttcacctgc ctcaccccgg actcacgcgc caaaggaaaa
     2821 cgcctgcttc tggataagct gcaagtgagc ccggcgggcg agtagcggga tcacgaaggg
     2881 ccgccagccg cgaatttggc ggcggtcacg cgccaaccgg cgggcgttcc cgttgggggc
     2941 ccattcaggc gcgctggcag cgcgggcaat aataggtgct gcgggccgct tgcacgcgcc
     3001 gccgaatggg ggtcttgcag cgcgggcaag gctggcctgc ccggtcatac acctgcaaac
     3061 gctcctcgta gtaatccggc gcgccagggg cgcggccaaa gtaaaacagc cggtcggatg
     3121 ccccctggcc ggtgtaattg agcggcacgg tgctgccgcc ggcaatggcc tgccggagca
     3181 cggcgcgaat agcgagccga agccgcctga cctggctttc gctcaggcgc cgggccggca
     3241 acgccggcgc gatgcccgcg cgaaagagag cctcgctggc ataaatgttt cccacgccgg
     3301 caagcagcga ttggtccagc agcttgacct tgatggcctg ccgggactgc cgcagagccg
     3361 cgacgaaggg ccgcagcgca aaggcgcttt ccagaggctc cggcccgagg ttccgccgcg
     3421 gggccgccgc cagggtcaac cgtccgaagc ggcgcgaatc ctcgaagata aagttctgcc
     3481 gccccaggcc cagaaccacc gccgcgtgcc gcggcaacgg cgttttggct ggcagcaaat
     3541 acattcgccc ggtcatgccc aggtgaccgg tcagcgttaa tgccccgcct tgcccggccg
     3601 gccggaggtg gaacaaaagg tatttccccc ggcgtgacac cgcgcggaac ctggcgccac
     3661 gcaatgcctg ggcgaaacgg cgtggagaat ccggccgcac cacctttgcc aggcgaacct
     3721 ggacggcgtc aatccggcgt cctttcaaga gcggcgccag gtggcgaacc agcacctcaa
     3781 cttcgggcaa ctcaggcata atgaaggacg gccggtttgc aggggcggaa gcgaggcatg
     3841 ggcgggtcag cgcggatgag cttgcgtggc ggctcgcagc acggccagca cttcctcgtc
     3901 cgtgttttgc gcaaaggact catagtactg gcccacggca gcaaagtccg gggccacatg
     3961 cagcacgcgc acttcgtcgg ccacctgggc gaggcggtta accgcaccgg acgaagctac
     4021 gggcgcggcc acgacaataa agccggcctt gcgcgccttg gctgacaaga ccgcggcctc
     4081 ggttgtggcg ccggtcgcga ggccgtcgtc cacgagcaga acggccttgc ccgccaggac
     4141 cggggcgccc tcgggatgga attgcgcctg gcaggcgcgg agacgttctt tttcctcttc
     4201 gaggacggcc gccagctccg cgcgaactcg cgggttgggg ccgactgcgc gttcatcgag
     4261 cagggttatg tcgccttcgg ccagcgcgcc gacggcaaat tcacggttcc aggggtgccc
     4321 gattttgcgg acagtgatca cgcccagggg ccggcggaga gcttgcgcca cttgggcggc
     4381 cacgattacc ccgccgcgag gcagccccag taccaggtcg gcttgaactg cctgcgtttg
     4441 cagcagattt gccagtttga gccctgcgtc ttcgcgtgaa cgaaatatca tttttgcggg
     4501 gaaaggcagg gaagggggcc ggatgtcgcg ccggggtgga gccggcgccg ggcggccgcg
     4561 cgcgctcagt ccgccacaag ggtgccgatt tgttcgccca ggataatccg cttgaggttg
     4621 ttcggctggg agaagttaaa cacgatgatc ggcattttgt tgtccatgca caaggagaag
     4681 gccgtggagt ccatcacctt gagccgctgc cgcaacgcgt cggagtagca gatttgcgaa
     4741 taacgctttg ccttcggatt cttctggggg tcgtcgtcgt agatgccgtc caccttggtg
     4801 gccttgagca ccacgtcagc gccgatctcg ttggcgcgca gggcagccgt ggtgtcggtg
     4861 gagaaatacg ggttgccggt gccgccggcg aagatgacga cgcggccttt ttccaggtgg
     4921 cgcacggcgc gacggcggat gaagggctcg gcgacctgcg agatgctgat ggcgcttagg
     4981 acgcgggtgg gcacctccag cttttccagc gcgtcctgca gcgccagcgc gttgatgatg
     5041 gtcgccagca tgcccatgta atcgccggtg gtgcgatcca tgccgcgttt gctgccacta
     5101 agcccgcgaa agatatttcc gccgccgacg acaatgacga tctgcacctt gagcttccgc
     5161 acggcgcgga tttgcccggc catgctccgc accgcttgcg gattgatgtt caggccggtg
     5221 gggccaccca gggcttcgcc gctcagtttc agaaggatgc gcttatattt agggcgtttt
     5281 gcttgtttca tagcttcgat ttatgtggcc gcagtcaatc cgcgcagaat tgtttcgctc
     5341 ccgggcggaa aagtcaaatc aaagcccagg ccgcggcgcg gcgttttcaa gcggcccggc
     5401 gagagggatt atttcgcagc ccgcttgccc cgccaggaac cgaggggcag aacattggcg
     5461 cgcgcggccc aggcctccca gcgggcggat aattccttga ccttgtccgg gtagcggccg
     5521 gccaggtcgt tcatttccgt tcggtcggcg gtgaggtcgt agagttccca cggctggccg
     5581 gccttggcca cgagcttcca ttggccgtcg cggatggcgc ggttgctttc gtgttcccag
     5641 cagaggggct gtgcgcggtg gaggggtttt ccgtccaggg ccggacgcag gctgacgcct
     5701 tccaggggct ggattttctg gccgtccttt tcggcggggt aaacagcgcc tgccacatcc
     5761 acgcaagtgg ccatgaggtc aaccaggtgg gcgggctggg tgacgagttg gttgcggcgc
     5821 gaggaagcga tgccccgggg ccaatggaca atcagggggg tggcgatgcc gccttcgtgg
     5881 acccagtgtt tgtattcgcg gaagggagtg ttcgagacat tggcccagcc gcggccatag
     5941 gcgacgtaag tgtcctccgg gccgggcatg acctccgggc cggtgcggac ggggcgcccg
     6001 tcgcgggttt gcatcggcgg ccaaatcttt ttttggagtt catccggggc cataggctgg
     6061 caggtcatgg ttttgatttc gtcggcgttg gatttgcgcc ccatgggttc ggcgcagccg
     6121 ccgttgtcct gcaggaagag aaggagcgtg ttgtcgaagc gattttgccg tttcagctcg
     6181 gcgacgattc gtccgatgcc ctggtccatc cgatccacca tcgccgcata cacttccatg
     6241 caacggcttt cccaggcccg gttggtgacg gcatcccagt cttcggccgg cggcggcagt
     6301 tgccaggccg ggtcaatcag ccccagttcc ttgagccggg caaagcgcgc ggcgcggatg
     6361 gggccgtaac cgccgtcgta gaggccccgg tatttggcga tttcctcggc gggcgcgtgc
     6421 atgggccagt gcgcggcggt ataggccacg tagaggaaaa agggctgggc cgagtgatcg
     6481 cgggcgtggt cgcggatgaa agtgatggcg ttgtcgctga tggcatcggt gtagtagaag
     6541 cgggtggggc gatattcggg atcgttatcg ggggtgatat aggtattgcc gcggcagagc
     6601 gtggtgggat cgtagaagct gccgccgccg gtaatggtgc cgtagaagcg gtcgaagccg
     6661 cgctgaagcg gccaggtgtc cttggggccg gtgggggcaa taatgtttgc cacgtgccat
     6721 tttccgctca ggtaggtgcg atagccggcc gggcgcaggg cttcggcgat ggtcacgcat
     6781 cgccggttca ggtcgccggc gtatccgtct tcgcgcccgc tgccatgccc ggtcatgtgc
     6841 cccatgccgg cctggtgggg ataaagtccg cttagcaggc tggcccgggt ggggcagcag
     6901 cgcgcggtgt tgtagaactg ggtgaagcgc aacccctctt tggccagggc gtccagattg
     6961 ggcgtgcgaa tctcgctgcc gtagcatccc aggtcggaga atcccatgtc gtcggacata
     7021 atgaggacga tgtccggctg tgatgattgg gcggaagcgg aggcggggcg gggaagggat
     7081 tttcgccatt ccagagcctt ggcgcccagg cggtcggcca gggccgggtg tgcggccgcc
     7141 tggttggtcg tttccccggg atcggtggcc acgttgtaga gttctcgttg acggccatcg
     7201 gcgttgagca gcaatttcca gtcgccttcg cggatagcca cattctggct gcggttggtt
     7261 ccttttggat aggcgaaggc atgttcattt cggccgtatt cccagaaaag cgcctttgcg
     7321 cgggcggcga ccgggcggcc cagcaaagcc gggctgaggt tttcgccgtc cagggcagcg
     7381 ccggcgggca gaggcgcgcc tgccagcgcg cacaggctgg ggaagaggtc ccacgcgctc
     7441 agcacggtct ggtcgtcagt gcgcccggcc gggacttgcc cgggccagcg cactagaaac
     7501 ggcatgcgga tgccgccttc gtagaggctg agcttgcttc cgcgcaagcc tgccgtgcgg
     7561 cggccccgga aagtgggcag ggggccgttg tcgctcgtga agatgagcag cgttttttcg
     7621 tccaggccca attccttcaa cccggccagc aaccgcccaa tctggcgatc gtattcagcc
     7681 atcacgccga tgaagttgcg ctcggtctgg gcgccgttgg gaaattcgct gagtcgttcc
     7741 cggttgggca cccagggggt gtgaacgtcg tccggccaaa ggttgacgta gcagggttgg
     7801 ccgcgatgac ggttcaggaa gtccagggtc cgatccacga agtaggcggt gcggtcccag
     7861 cgctggactt tgtcctgcgc cgaccagatc cagttggtag cggtgaggtc tggatgcgga
     7921 tccgggcttt cgtaggtgct ggcatgttca tcaaaaccat aggctgggaa agggggggcg
     7981 ttggtcacgt cgcggccacc gcccatgtgc cacttgccga agtggccagt ggcatacccg
     8041 gcggctttga gagcgcgcgc cagcgaagga gcactggggt ccaggaaatc cgcttgttcg
     8101 caggcgtggt tgccctggcg tgtttgcagg aaactggtga tgcgccagcg ggcgggatgc
     8161 attccggtga gcaaccccgt gcgggacggc gagcagatgg gcgaagccgc gtagtattgc
     8221 gtgaagcgga tgccttcccg cgccatgcga tcaaggttgg gcgtgggaac caggtcgccg
     8281 ccataacaac ccagatcgcc gcagcccatg tcgtcggtga ggaggacgat gatgttgggg
     8341 cgggtgatgg gtggggtggc ggcttgcacg acgccggggc ttggcaggta agcaccggcc
     8401 atggcggcca gtatcatcaa acgtggcaga aaaagaatcc cggcgcgcat ctgctgccgg
     8461 attagccgtg ggcttggagt cataggcatg ttgggcaatg gcgcaacggg caatagatta
     8521 aaggcaaccg gggccaatta acagcaaatt ctccagcgca actggtttcg gcgagtgggg
     8581 aaacaaccgg ccgggtttca gaggccgggc gtgccgccca taggcgggtt tgcctctcat
     8641 ggggtgaaca ccccatagcc gccagagggc ttatagtata gatagatgta tgaaacgact
     8701 atggctcgaa taggaggcga gccaagtggc actacgcgga gcggcccgac aaagggccgc
     8761 tcactttttt tacccatagc gcatgaatta ggggcgattc gcccttgcct cggctcccgc
     8821 aagccaggca gcaccgctga tacgaaacct cggcgttccc gattggggcg gttaaggcaa
     8881 ccgctgcacg cggtagtagc gttggctcag gtttgtctcg gcatccagga attggaagga
     8941 gggcccggcg gtggtcagat tggttaacgc ctcccagttc accaggttgg ttgtcacctt
     9001 aagggtgtag tcgcccgggc cgccgctgac ctgcaactgg atttggccgc cgggggtcag
     9061 ccggatccag tcaatttgca acggagcgga tggaatgaga gtgagttccg cttcggagct
     9121 ggccacggtg ccggcgagat tggagaccac gacagaatag ctgccagcat tttccgcctg
     9181 gacattccag caagtataag cactgtcggt agcggcggcc aggttggtgc cgttgaaacg
     9241 ccattgatac tccagcggca cggtgccggt agcgatcacg gtgaaggtgg cgttgctgcc
     9301 cgccgcgacg gcgatgcttt gcggctgggt gataataacg ggcggcacca ggacggtgag
     9361 gagggcgttc gaactggtca cgctgccggc cacgttcgtg acgatcgccg catacgcgcc
     9421 ggcgtcagtc ggttgcacgt tgatccgggt gtagctgctg gcggtggctc cggggatgtt
     9481 ggcgccgtcg aatatccact gataactcag cggcgcggtg ccggtggcgg tcacagtaaa
     9541 ggtgatattg gtcccggcga tcacggtcgc gctttgcggt tgggcgataa tgcccggagg
     9601 cacgctaatg gcgagggtgg cgttggaact ggtcacgctg cccaaggcat tagacacgac
     9661 gacggcatag atgccggcgt gcgctgcctg caccggggaa cgcgtgtagt ggctgtcagt
     9721 ggcgccggcg aggctcacgt tgttgaactg ccattgatag ctcagcggcg ccgttccggc
     9781 cgccgtgacg gtaaaggtgg cgttgccgcc ttcatttacc gtgaggcttt gcgggtgctc
     9841 caggatggtc ggcggcgcca gtaccgcgag cgccgcaatg gggctggtgg cgccgcccaa
     9901 ggcgttggat acgatgactg catagttccc accgtccgaa ctttgtacgc cggccaggtg
     9961 gagcgcggac gtggtggcgc cgtcaatatt cgttccattc cgctgccatt ggtagcttag
    10021 cggcggggcg cccgcggcca tgacggtcag gctcacgttg gagttcgcgg cggcgacctg
    10081 gccgcgcggc ggcacggtga tatacgggcg gccgtcgttt accagcgcca ggttgtgata
    10141 gccgctgctc gagataccga tgacgttggc cacactgttt ggcgtggaag tttggccgta
    10201 gctattatcg ccccaggcca ccaccgttcc gtctgctttc aaggcgaggc tgcagtagcg
    10261 tcccgcgcta attgccacgg cattagtcag gccggcgggg acattggttt ggccgtagct
    10321 gttgtcgccc caggccacca ccgttccatc agctttgagg gccaggttgt ggtagccacc
    10381 ggcggcaatc atcgccacgt tggtcagtcc attggggaca atcgcctggc catagtgcgg
    10441 attgccggaa gtggtagtgc cggctcccca ggccaccacg gtgccatctg ccttgagggc
    10501 caggctgtgg tagccgcccg ccgcaatgga aattacgttg ttcaggccac tgggaatcat
    10561 cgcctggcca tagtgcggat tgccggaagc agtagtgccg gctccccagg ccgccacggt
    10621 gccatcggcc ttgagggcca ggctgtggta gccgcccgcc gcaacggcga ggacactgcc
    10681 caagccgccg ggaatcattg cctggccgta gtgcggactg gctcccgagt tattcgtgcc
    10741 cgcgccccag gcggcgatgc tgccatcgga gcggagcgcg aggctgtgga agtagccagc
    10801 cgcaatcccc accacgttgc tgagactgtc gggaaccacg gcctgcccga gatgaggatt
    10861 agcccccgcg ttggtcatcc cggctcccca ggcggcgaca aggccgtctg tccgcaatgc
    10921 caggcagtgg taaaagccgg cggcaattgc cgacacggtg tttagcccgg caggaatatc
    10981 cgtctgtgtg tagttatttc ggccccaggc agccagtggc gccagcccca ccaacgcatt
    11041 tgagctggtg gcgttcccgt aggcattttc gacgaagagc gagtagtctc cgcagtcgtc
    11101 aatctgcatg tcggagattg tcagggttgc tgtggcggcg ccgctgattt tgccgccatc
    11161 cgcgaggtta attcctccct tacgccattg gtaaacgagc ggcgcgggac caaccgcggc
    11221 ggcagtcaac tgcgcggagg agcccgcgac gactttctgg ctgaccggct gggatactat
    11281 gaccggcgga aattggccga gcaaggtggc gttcgagctg actatctggc tgttagagtt
    11341 gctcaccacg actgaataat tgcccatgtc catcgcctgc gcattggcca cagtcagggt
    11401 gggtgttgcc gccccgctga tgcgtccgcc gtcaaacaga tccacgcctt ctttgcgcca
    11461 acgataactc aaggtgggcg aacccaccgc gctgacggag aaggtggcgg tggcccccgc
    11521 cgccacggat tgattttgcg gctgggtgac gatgaacggg tccatcacag tcagggttgc
    11581 aatcgaactg atgacggtgt catacggatt ggacaccatc accgcgtact cgcccgccgc
    11641 cgtcgcctgg gcgttgaaga ttgccaggcc ggcagccgtt gccccgttaa tattggtgcc
    11701 gttgaactgc cattggtagc ttaacggcgc ggtgccgctg gcggtcacgc tgaacagggc
    11761 agtcatgccg atcccgaccg tggtatccac tggctgcgtc gtaattttcg ccggcagggc
    11821 caccaccaga attgagggac tgctggtgac cgacccggcc gtattggtga tcaccaccgt
    11881 ataagtgccc gcatccgccg gctgcacatt gttgcgggtg taggtataga gtatggcatt
    11941 ggggatgttc gtgccgttga gccgccactg gtaatggagc ggcggactgc cggaggccac
    12001 cacgctaaag gaggcggcgc tgccttgcag gttggtctgt gtggccgcgg gttgggtcac
    12061 gatggacggc ccgactggcg gcggcggttc gtaaacgaac ttgtaaccgt cgctatacat
    12121 ccgggaactc gcgctgagcg tgccgccgga cttggtgaag gtgatggttg gcattgtctc
    12181 cccgggggag agcgtcatct ggccaacata gccccaggag ttggcatagg acgactggaa
    12241 gaccggggtg gtggccggca atccggagca cccgctctga gttatgctcg ccaccaaatc
    12301 cgccgaacaa ctggtagcgc aatgcgtcac gtaaaccttg taggcgccac ccgcgatggg
    12361 cagagtgggc cgcagcgtga ccgaaggggt tccactgccg ccggtggcat actgaacctt
    12421 cgcgggtgcg gtaaggcccg gggcagtgct cttgcaggtt gaaaacgcca cgaaccccgc
    12481 atcggtgtac ggcgggttgg aattcagcgc gccgttgggc aggtagcttt ccacaattac
    12541 gtccggcgcc gtaaccgccg tggtcgcaaa gacattgaca ccggagacgc cggtttggtt
    12601 attggcgttc ttcgatttaa cccggtaata ataagtcgtg taggggctca gaccgctcag
    12661 ggtgacgctg tggacataga tgggcgaact gttcgtgacg ctggtgccgt agttcgtgtc
    12721 caggccgtat tcgaccacgc tgtctgattt ttgatcggtc gtccaggtaa cggtggcgga
    12781 cgtggaattg atattatccg ccgaaacatt cgccaccacc ggcgcattgg tccagagggt
    12841 gatctcgtta gtggcgatgc tgggccaaat cgccgccgga tgcagttcgc atacccagta
    12901 attcactcct ttgaaccctc ccgcggtggc agggttggcg acgttcttga ccgcgtcgac
    12961 aaactcggtg ataagggccg gggtaattgt tccctccccg tgccagccct gcccgagcgg
    13021 cacaatgggc ttgatggaat tacgccattg cccatagagg ccgttttgcc agttgcggaa
    13081 atcagtgttc attttgttca cgcattgcgt gggcgtgacg ccaatctgga tgaaataaac
    13141 ctgcggcatg acggcgtcgc agtagtagcc gaattccttg tacggaaaag tggaatgcac
    13201 gctgatataa gggaaaggcg catgcgccag gaatttgttg ggccagttcg agcgcaccat
    13261 ggagcagagc tggatcgcct tggcggtatt gttgggcagg ttatgggact cccactcgat
    13321 ctccgcgtca aagacaaagc catcggcccc ctggttgaac acccaatcag cgacggcgct
    13381 ttcgccgggg atatccagcc cgtccgagcg cgtgtagccg aagatcttca acccggcgtt
    13441 gtgggcggcg tccaccaggc tgctgctgaa ctgggggctg tagcccggaa ccgagaaaag
    13501 cgccccgccc gtgccggctt tgacgatgat atattgcatg ccctgattct tgaggtaagt
    13561 catcaagctg ggcacgtcgg tcaccgacgg cacattgccg ttgcattggg cgactgcgtg
    13621 gtgcaactgg taaatccagt cgcccttccc aaggttggcg gggtcaattc ctccatcccg
    13681 gattactccg taggcagaat gggttagttg gcaggctatg agcagagccc caaggcgggg
    13741 agcaacgcga atggcacgag tcaacaggcg cataattgcg gagcaatatc acgcaataaa
    13801 accgtgcggt caatgccttt gtgcggaaat ccattatgta acagggtctt tggttttgta
    13861 gatcataagc cgaggcggtc gaggctgcgg attgggcggc ggttaagcgc tttggggagg
    13921 ataactggcc gtgcgccagg gcccgggttg ttggaaatag ataaggctgg ctgctttgcg
    13981 cggaaggaga ttgttgccgc ccttttgcct cgattgtttg ggccagtttc tgccgaccgt
    14041 acaaaggatc agcggtaatg agcttgctat tgcagaaact caggcgccga taacgaagga
    14101 ttctgcccgc caccgccaac cccaaaaaat tgcccttgcg cgaagcctcc ccgcccgcca
    14161 gccttcccgc gtgggctcgg ggtggcggcg gcggggactt ccagcccaag gaatggcagg
    14221 aagccgagtc gccctccggg gcgatgacag aacgacaata aaaaaacaac aaaaaggaac
    14281 tacgttatga aaatactatc tgctccgcgc acgggcagcc tgggcgcgga aacatattac
    14341 caatccccct tcggcgtgtg cgcccggcgg cgcaccgttc cccgggaccg gccttctaac
    14401 cgcaaagcgg ccgcacggtc gtatttcggg atctcctcgc gggagtgggg cctcaaactc
    14461 agcgaagcgc agcgcgaacg ctggaacgcc gcggcgctgc acgtccccag ccggccctgg
    14521 atggggcagt att
//
LOCUS       DAOPQG010000090        14441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_4903_length_14441_cov_14.797790, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000090 DAOPQG010000000
VERSION     DAOPQG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14441
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4903_length_14441_cov_14.797790"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(512..1183)
                     /locus_tag="PKI07_10310"
     CDS             complement(512..1183)
                     /locus_tag="PKI07_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOC51483.1"
                     /translation="MEPNRVFWGMLSRRQCLVPTRRGWLLLALGVGVLVFAVLRGAYP
                     FLALNAPAPGGILVVEGWVPDYALAAAGAEFRRHHYAKLLVTGIPLEQGAPLSEYKNY
                     AHLGAATLIQLGLSTNDIQAVPAPLTRRDRTYATALSLKAWLREQQVPPTKVNLVTTG
                     PHARRSRLLFEKALGRDFTVGVLSVPGRDFDAQHWWRSSQGVRVVTGEMIAYCYVRFF
                     FRPNE"
     gene            1338..2852
                     /locus_tag="PKI07_10315"
     CDS             1338..2852
                     /locus_tag="PKI07_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOC51484.1"
                     /translation="MRTLLLLSAALMLLTPEGGPAARADEPPAASVAFVSRDRVVRNA
                     LALMNAGQFQKAEALLEKADRPGDAATLRARTEAREILRRTRIEYSLDAPGLLAKVRR
                     SVPDATAEEVERWARDSGARYRVIDGRKLYFRREPQNIFLFCAEARERRARAGRAPAE
                     PQWKLTDHLQAIVAEAERTGQAEVHPVRHRITFTLTMQPNLPGVKTGSLVRVWLPYPQ
                     EYRQQREVRLIRATPEPRVIAPNAIDGKQVGGAPQRTIYMEQRVTDPGQPLKFEEVFA
                     YTCYAYYPRLEAAKAQPLPADWGRAYLEERLPHIAFTPELRRQAAAIVRGETNPLTRA
                     LRIFRWVSTHIKWNAEDEYGTIPSFARKGFAAGRGDCGVQATVFVTLCRLAGIPARWQ
                     SGLEIKPTPTWGMHDWAEIYVAPWGWLPVDVSYGVQPSSDPRIADFYCGHQDSYRTIF
                     NLDWGRELVPPKSSLRSEPADFQRGEIEVDGRNLYFDQWDYSIAVECDPGPSPR"
     gene            complement(2922..4391)
                     /locus_tag="PKI07_10320"
     CDS             complement(2922..4391)
                     /locus_tag="PKI07_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51485.1"
                     /translation="MIADVSRRCFLKSTAAGVVSAGALAALAERLAAMSPAPALGPKS
                     KVRIGKIYLGHERPGWPSWQVDLQADVQRFEAQLARLRAGLADVEFVEGGVASNAAQV
                     AAAKEKFQNVSGILAIHLTLGVGGQIQSLLELGLPVMLFSLPYSGHEWHTIASWQRQG
                     KRIEALTTSRYEDVLAAIRPFRAIHRLKEARVLNVSTGPADAAYCQALKEKFGTEIRS
                     LLLPDLQRACAAADRGEAMADMRRWIKEARKIVEPTREDILKSSVMYIAMRDLLAQHG
                     AVAITMNCLGMGLIDRGLGYPCLGFARFNDMGLGGVCEADLKSTMTHLIFSYLVGKPG
                     FVTDPVLDEASSTIIHAHCVAATQMLGPGTPASPYQLRCHLEDGRGVSLMVKLPIKRK
                     VTMARLLGTDKMLFSTGDAVDSPFVERGCRTKLTVKVENIEKFLENWSSGLHRVIFYG
                     DHRRDVQRYCRFAQIRLIREGTDDPQKVDGLDWIPHVHA"
     gene            complement(4466..5590)
                     /locus_tag="PKI07_10325"
     CDS             complement(4466..5590)
                     /locus_tag="PKI07_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOC51486.1"
                     /translation="MPPSTMRAVVYRGPNTLRLETVPVPRIGPGELLVRVAACGVCPT
                     DIKKIQYGLLPPPRIFGHETAGTVVRAGARVRGFRAGERVALHHHVPCLQCHACRHRA
                     FAQCAQYQRTGITAGFAPAGGGYAEYVRVMPFVLPGVVRIPARNSFIEGALLEPVNTV
                     LKAIRRLALLRGDSVLVAGQGPIGLIFTRLLALAGMRVIATDLLPARLKLAREFGAAA
                     VHCPAGPAPSPARRSAPRAPRPAAPNLATLIPRFTRGRGLDAAVITVPADDVVGEAQA
                     LVRGGGQVLLFAHTRRGTATPLDLATVCVDEKDLIGSYSADFQLQAETARLVFSRQLD
                     VRRLVTHQFPLAQTARAVELATHPTPDSLKVVVTPGAPEV"
     gene            complement(5591..7198)
                     /locus_tag="PKI07_10330"
     CDS             complement(5591..7198)
                     /locus_tag="PKI07_10330"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOC51487.1"
                     /translation="MSTPDLVILNAAVHTMDAARPTADAVAIRGSRIAALGTTDEIRA
                     LAGPRTRVIDAGGKLVLPGFNDSHTHFLMGGFSLASIDLREAASPEELTRRVREYVRN
                     VPKGQWILGGNWDHEKWPGAPLPTRQMIDAATPDHPVLLRRLDGHMALANSVALQRAG
                     ITRETPDPPGGLIVRDPGSGEPTGILKDSAADGLERLVPEKTFAEKHAAARAATEFAA
                     RMGVTSLADLSADADVGLYQYMRERGELKTRIYAVRSIVSWEVLAQTGVRAAFGNDWL
                     RIGGLKGFADGSLGSTTALFFEPYRDAPDTRGLFFEQMLPEGIMLQRVAAADKLGLQV
                     MIHAIGDRANACILDLYREVAEQNGPRDRRFRIEHAQHLRAADIPRFGRQKVIASMQP
                     YHAADDGRWCEKRIGTERSRGTYAFRALLDSGAVLAFGTDWTVAPLNPMLGLKAAVTR
                     QTLDGKHPDGWFPDQKITLDEAIRAYTVGSAYAEFAEHAKGALSPGKLADLVMLDRDL
                     YRINPADIDQARVLLTVVDGRVVWEAK"
     gene            7419..8882
                     /locus_tag="PKI07_10335"
     CDS             7419..8882
                     /locus_tag="PKI07_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOC51488.1"
                     /translation="MSARDVSKDPVWRGEELGQPIPALPHAVSVALPRWEDVVGYEEK
                     KPEVVNALAGGYPRFFIHAEVRELARQLGDGRPCLPFPSPRAAGLCAEFIERTSGQQA
                     KVLTGRGLSGVVTSDAGSAALRAFWQHTGLIVSSRQAAAHLAGRAAGPEELAALPALR
                     RQLAGFYDCAEDDVFLAPTGMAAQFAALQAVRQRTPGRPTAQLGFPYVDTLKLQQKLG
                     HGGILLHRLGSIEQELRDLLSRQPLAACFSEIPGNPLLGSADVRRLHPLLRAQRVPLV
                     VDDVVATPANIDLSGCADLIATSLTKFMVGTGNAMGGALICNPRSPLYAELKPIISAR
                     HEELLWGEDAVVLAAQTRSFPERMKRHNQNGLWLAERLRRHPAVERVWYPKWEFAEAY
                     EAVRRPEGGWGALITFLPKEGETRSPGIYDRLEFCKGPSLGTVFTLACPFMLLAHYTE
                     LEWAESCGVPRSLIRLSVGLEDPEDLWRRLERALTAR"
     gene            9075..10514
                     /locus_tag="PKI07_10340"
     CDS             9075..10514
                     /locus_tag="PKI07_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOC51489.1"
                     /translation="MVSEERGLPDTFDPRTGRNVQWSAELGTETHATPIVAGGRVYIG
                     TNNGHPRDPAQQGDRGILMCFDERTGRFLWQMVFLKRDEDIYFDWPRSGIASPVTVED
                     GRVYFVNNRGEVICLEPPPIPEKAEGGAAKTETANLPAPRILWQFDLTAGAGIWSHDG
                     AHSPILINGDYLYLNTGTGVDNTHRRIRTPDAPSLVVLDKRTGRLVARENEGIAPNIF
                     HATWAGPALAAINGQPRLFFAAGNGIVYAFETLPNRGPENRKTRTAPASPSGSPTGAA
                     ASRSPAFLQQVWRFDFDPAAPKTNIHLYVSNRRESPSTFYGMPVFHHNRLYLAGGGDL
                     WWGKHEAWLNCFEATGTGDITPHALAWSYPLQKHVLATPAIWRGLVFIADCGRMFHCV
                     DAQTGQPCWTHELKGEAWASPLVADGKVYLGTRGGSFYVFAAERDKKVLATVALERPI
                     SATATAANGALYVATMDRLYALRQGVGAQ"
     gene            10705..11793
                     /locus_tag="PKI07_10345"
     CDS             10705..11793
                     /locus_tag="PKI07_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOC51490.1"
                     /translation="MGHVKLHIPGPVEISEKTYRAFCQPMIGHRGQGFKDLYARIQPQ
                     LQQLLYTRQLVYLSTSSAWGVMEGAIRNLVSRKVLNCMCGAFSDKWLDVSRRCGKDAE
                     GLQVPWGSPIRAAEVDRKLATGQFDALTVIHNETSTGVMSPLAEIAALKQKYPDVMFI
                     VDSVSSMTAVPLKFDELGIDVLLAGTQKALAMPPGLALFVCSPAALAKAATVKDRGYY
                     FDFVEFQKNAAQNMTPSTPSISHIYALESKLQDIFAEGLEARYARHRRTNQLTRDWAA
                     RHGFTLFPDPGFESVTLTCVNNGARPGGRVVDAEKLVKLVKDQGFLINGGYGKIKGTT
                     FRVSNMGDETEATMNQLFAAMDNAMKQL"
     gene            11790..14000
                     /locus_tag="PKI07_10350"
     CDS             11790..14000
                     /locus_tag="PKI07_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOC51491.1"
                     /translation="MIAVWLGGLRGAVLRAGLILSLPALAADLYVSPGTGRDSNPGTR
                     ARPFATLERARDEVRRLRQDRSSGGGPFTIWLEGGDYLRTNALELSAADSGTARAPVT
                     WRARKGQAVRLMGGRKLSGFAPVTDPAILSRLSPNARTNVVQADLRALGITDFGQMQS
                     RGFSRPLAPAHGELFYDGRPMTLARWPNVGHWKHLAGYPESGAIKNEWGREVGRLEDG
                     FTYTGDRPRRWADLSGLWVHGYWAWDWANSYERVAAIDRDRRLIRTAAPYGLYSFRKG
                     QRFYFLNVLEELDQPGEWFLDHGTGRLYFWSPAPLPLPGVEARRAPGAKPRPSDSPAA
                     PEILLSVLAEPLLRLTDASHIIFRGLTLEATRGNAVEIRGGASNRIAGCLIRNIGNYG
                     VTISGGAGHGVQSCDLFDTGDGGVMLDGGDRQTLRPGGHFVENCHFARQGRWSKCYVP
                     AVLISGVGQRAAHNLIHDHPHCALLFNGNDHCLEFNEIHHIALETGDVGAIYTGRDYT
                     SRGNRIRHNFIHDTGGVGMGSMGVYMDDCVSGAGIVGNIFYRVRRAVFLGGGRDHQVL
                     NNIFVDCNYAVELDGRGLDASPVWRNMVDNTMRHRLTEVPLSLYRERYPALQTLDSYY
                     GPPGGPALAGPNFQGVPPEHNVVARNVCVGKWLHAYWHASPEMIQLENNLTNAAAAFR
                     RPPGNPPQARDFALRRNSPARDLGFQPLPLDQIGLYRDKLRARLPAPLSSKTRR"
BASE COUNT         2357 a         4812 c         4869 g         2403 t
ORIGIN      
        1 aggtgtttat cgtcaccagc cagacgcgga acggctggaa agggccgaac tgggtccaca
       61 gcgccatcgt cccgcccccg accacggcca aagctcagca agctactgaa aaggcaccag
      121 ttaagaaagc gaagcccgcc tccacctcgc cctcttccac tgcccgcgct accgtttctt
      181 cttcgtcgtt tcaggctatg tacaagggga gtacaccgga ggcgcggcag gagcgcatgg
      241 aaaataagca tgaaccccct ttgagcatcc ttggtacacc ccttgtacac ggcctattgg
      301 gagccatagg ctggctgggg aggttggggc gattgggaat ggcgggaagg cgtgcttaag
      361 ggaggtcgaa tcggggaaat attgagcgat cgtccccggg ctttccgggg cggcggccgg
      421 agtgtgcctt gtggcggcgg ctggagcgct ggcgcctatc ccagtgattc cggcgcgggc
      481 gtgcctttag cggccggaag tcttctctgg actattcgtt cgggcgaaag aagaagcgga
      541 cgtagcagta ggcgatcatt tcgccggtga cgacgcgcac cccctggctg gaacgccacc
      601 agtgttgcgc gtcaaagtcc cggcccggga cggacagcac gccgacggtg aaatcgcgcc
      661 ccaacgcctt ttcaaacagc agccgcgagc ggcgcgcatg gggaccggtg gtgaccaggt
      721 tcaccttcgt cggcgggact tgctgttcgc gcagccaggc tttcaagctg agcgccgtgg
      781 cgtaggttcg atcccggcgc gtcagcgggg cgggcacggc ctggatgtcg ttggtgctca
      841 agccgagctg gatcagcgtg gccgcgccga ggtgggcgta gtttttatat tcggacaaag
      901 gcgcgccttg ttccagcggg atgccggtga ccagcagttt ggcatagtga tgccgccgga
      961 actcggcgcc ggcggcggcg agcgcgtaat ccggcaccca accttcgacg accaggatgc
     1021 cgcccggcgc gggggcgttg agcgccagaa aggggtaggc gccgcggagc accgcgaaga
     1081 ccaggacgcc cacgcccaaa gccagcagga gccagccgcg ccgcgtcggc accaggcact
     1141 ggcgccggga caacattccc caaaagactc ggttcggttc cataagcatc gggcctgggt
     1201 gggtatatac cccaaggcgg cggccaggtc gagtttgcgc cgcggaaatc gcggcttatt
     1261 agcttgattg cgggccggag cggcgcatat tggcgtggat gtcggatcaa acctgcataa
     1321 gcaacccttg cccgcgtatg agaaccctcc tccttctgag cgccgccctg atgctattaa
     1381 ccccggaagg ggggcccgcc gcccgggcgg acgaaccgcc cgccgcctcc gtggcgttcg
     1441 tttcccggga ccgggtggtg cgcaacgccc tggccctgat gaacgccggg cagtttcaaa
     1501 aggccgaggc gctgctggag aaggccgatc gcccgggcga tgccgcaacg ttgcgcgcgc
     1561 gcacggaggc gcgggagatc ctccggcgca cccgcattga atactcgctc gacgcccccg
     1621 gcctgctggc caaggtcagg agatcggtgc cggacgccac ggccgaggag gtcgagcgct
     1681 gggcgcggga cagcggcgcc cgctaccggg tcattgacgg ccggaaactt tacttccggc
     1741 gcgagccgca gaacatcttt ctgttctgcg cggaagcccg ggagcgccgc gcccgggccg
     1801 gccgggcgcc cgcggagccg caatggaagc tcaccgacca tctccaggcc atcgtcgcgg
     1861 aagccgagcg caccggccag gccgaggtgc accctgtgcg ccatcgcatc acgtttaccc
     1921 tgaccatgca gcccaacctg ccgggcgtca aaaccggctc gctggtgcgc gtgtggctgc
     1981 cttacccgca ggaataccgc cagcagcgcg aggtgcggct catccgcgcc acgccggagc
     2041 cccgggtgat cgcgcccaac gccattgacg gaaagcaagt cggcggcgcg ccgcagcgca
     2101 cgatctacat ggagcagcgg gtgaccgacc cggggcagcc gctgaagttc gaggaggtct
     2161 tcgcctacac ctgttatgcc tactatccgc ggttggaggc ggcgaaagcg cagccgctgc
     2221 cggcggattg gggccgcgcc tacctggagg agcggctgcc gcatattgcg ttcaccccgg
     2281 aactgcgccg gcaggccgcc gcaatcgtgc gcggcgaaac caacccgctg acccgggcgc
     2341 tcaggatttt ccgctgggtc agcacccaca tcaaatggaa cgcggaagat gaatatggca
     2401 cgatcccgtc cttcgcgcga aaggggttcg ccgccgggcg gggtgattgc ggcgtgcagg
     2461 cgacggtgtt cgtcaccctg tgccgcctgg ccggcattcc ggcccgctgg caaagcggcc
     2521 tggaaatcaa gcccaccccc acctggggca tgcatgattg ggccgaaatc tacgtggcgc
     2581 cctggggctg gctgccggtg gatgtctcgt acggcgtgca gccgtcgtcc gacccgcgga
     2641 ttgccgattt ctactgcgga caccaggaca gctatcgcac gatcttcaac ctggactggg
     2701 ggcgcgaact ggttcccccg aagtcctccc tgcgctccga gccggctgat tttcaacgcg
     2761 gcgagatcga ggtggatggc cgcaatcttt acttcgacca gtgggattac tccatcgcgg
     2821 tcgagtgcga tccggggccc agccccaggt agggcttccc cccgcgccgt tgcggcggcg
     2881 gcaccggccg ccgccggctc ccggcccggc gcgctgcggg ctcaggcgtg cacgtgcggg
     2941 atccagtcca gcccgtccac cttctgcggg tcgtccgttc cttcgcggat gaggcggatt
     3001 tgcgcaaagc ggcagtagcg ctggacatcc cggcggtggt cgccgtagaa gatcacccgg
     3061 tgcagtccgg acgaccagtt ctccaggaac ttctcgatgt tctccacctt gaccgtcagt
     3121 ttggtgcggc agccgcgttc cacgaacggg ctgtccaccg cgtcgccggt ggaaaagagc
     3181 atcttgtcgg tgcccaggag gcgcgccatg gtgactttcc gtttgatggg cagcttcacc
     3241 atgagcgaca cgccgcggcc gtcttccagg tggcagcgga gctggtaagg cgaggccggg
     3301 gtgccggggc ccagcatttg cgtggccgcc acacaatggg cgtggatgat ggtgctgctg
     3361 gcttcgtcca gcaccgggtc ggtgacaaag cccggtttgc cgacgaggta ggagaagatg
     3421 aggtgcgtca tggtggactt gaggtccgcc tcgcagacgc cgcccagtcc catgtcgttg
     3481 aagcgggcaa acccgaggca gggatagccc aggccgcggt caatcaggcc catgcccagg
     3541 caattcatgg tgatcgccac ggcgccgtgc tgcgccagca ggtcgcgcat cgcgatatac
     3601 atcaccgagg atttgaggat gtcctcccgg gtcggctcga cgatcttccg ggcttccttg
     3661 atccagcggc gcatatcggc catcgcttcg ccgcggtcgg cggcggcgca ggcccgctgc
     3721 aggtccggca gcagcagcga gcggatctcg gtgccgaact tctccttgag ggcctggcaa
     3781 tacgcggcgt cggccggccc ggtgctcaca ttcaggacgc gcgcctcctt gagccggtgg
     3841 atggcccgga aggggcggat ggcggccagc acgtcctcgt agcggctggt ggtcaacgct
     3901 tctatccgtt tgccctgccg ctgccagctc gcgatggtgt gccattcgtg gccgctgtag
     3961 ggcagcgaaa acagcatgac cgggaggccc agttcgagca ggctttgaat ctgcccgccg
     4021 actcccagcg tcaggtgaat ggcgaggatg ccgcttacgt tctggaactt ttccttcgcc
     4081 gccgccacct gcgccgcgtt ggacgccacg ccgccctcca cgaattccac gtccgccagg
     4141 cccgcccgca gccgggcgag ttgcgcctcg aagcgttgca cgtcggcctg gaggtccacc
     4201 tgccagctcg gccagccggg gcgttcgtgg cccagataga tcttgccaat gcgcactttg
     4261 gatttcggcc ccagcgcggg cgcggggctc atggcggcca gccgttcagc cagggcggcc
     4321 agggcgcccg ccgacaccac gcccgcggcg gtggacttga ggaagcatct gcgtgaaacg
     4381 tctgcgatca tgccggcatt tctagtccgc cgcgcgcgcg gaggcaatgc caattttacc
     4441 cgcgcggcgg gcggcgggcc ggggatcaaa cctccggcgc gccgggggtc accaccactt
     4501 taagggaatc gggggtggga tgggtggcca gctcgacggc ccgggcggtt tgggcgaggg
     4561 gaaactggtg ggtgacgagg cggcggacgt ccagttggcg cgagaaaacc agccgggccg
     4621 tttccgcctg caactggaag tcggcggagt agctgccaat cagatctttc tcatccacac
     4681 acacggtggc cagatcgagc ggcgttgccg tgccgcggcg ggtatgcgcg aaaagcagca
     4741 cctggccgcc gccgcgaacc agcgcctggg cctcgcccac cacgtcgtcc gcgggcaccg
     4801 taatcaccgc ggcatccagg cctcgtcccc gggtgaaccg cgggatgagg gtggcgaggt
     4861 tgggcgcggc ggggcgcggg gcgcggggcg cggagcggcg cgccgggctc ggagccgggc
     4921 cggcggggca gtgcaccgcg gcggccccga actcgcgggc cagtttcagc cgggccggca
     4981 gcaggtcggt ggcgatgacg cgcattcccg ccagcgccag caggcgggtg aagatgagcc
     5041 cgatcggccc ctgccccgcc accagcacgg aatcaccccg cagcagcgcg aggcgccgga
     5101 tggctttcag gacggtattg accggctcca gcagggcgcc ctcgataaac gagttgcgcg
     5161 ccgggatgcg gaccacgccg ggcaggacga acggcatcac gcgcacgtat tcggcatagc
     5221 cgccgccggc gggcgcgaag ccggcggtaa tgcccgtgcg ctggtattgc gcgcattggg
     5281 caaaggcgcg gtggcggcag gcgtggcatt ggaggcaggg cacatgatga tgcagcgcca
     5341 cccgctcgcc cgcccgaaag ccccggacgc gcgcgccggc gcggacaacg gtgccggcgg
     5401 tttcgtggcc gaagatgcgc ggcggcggca ggaggccgta ttggattttc ttgatgtcgg
     5461 tggggcaaac accgcacgcg gccacgcgga cgagcaattc accggggccg atgcgcggca
     5521 cgggcacggt ttcgagccgc agcgtgttcg gcccgcggta cacgaccgcc cgcatggtcg
     5581 aggggggcat ttacttcgct tcccagacca cccggccgtc cacgaccgtg aggaggacgc
     5641 gcgcctggtc aatgtccgcc gggttgatcc ggtagaggtc gcggtcgagc atgacgaggt
     5701 cggcgagttt gccggggctg agcgcgcctt tggcgtgctc ggcaaactcg gcgtaggccg
     5761 agccgacggt gtaggcgcgg atggcctcgt ccagcgtgat tttctgatcg ggaaaccagc
     5821 cgtcgggatg cttgccgtcc agggtctggc gcgtgaccgc cgccttgagg ccgagcatcg
     5881 ggttgagcgg ggcgacggtc cagtccgtgc caaaggccag caccgccccg gagtcgagca
     5941 gcgcgcggaa ggcataggtg ccccgggagc gttcggtgcc gatgcgcttc tcgcaccagc
     6001 gcccgtcatc ggcggcgtgg tagggctgca tcgaggcgat gaccttctgg cggccgaagc
     6061 gggggatgtc ggcggcgcgc agatgctggg cgtgttcgat gcggaagcgg cggtcgcggg
     6121 ggccgttttg ttcggcgacc tcgcggtaga gatcgaggat gcacgcgttg gcccggtcgc
     6181 cgatggcgtg gatcatcacc tgcaggccga gcttgtccgc ggccgcgacg cgctggagca
     6241 tgatgccctc gggcagcatc tgctcgaaga acaggccgcg ggtgtccggc gcgtcccggt
     6301 acggctcgaa gaagagcgcc gtggtggaac ccaggctgcc gtcggcaaac cccttgaggc
     6361 cgccgatgcg cagccagtcg ttgccgaacg ccgcgcgcac gcccgtttgg gccaggacct
     6421 cccatgagac aatcgagcgg acggcataaa tgcgcgtttt gagctcgccg cgctcccgca
     6481 tgtattgata gaggcccaca tccgcatccg ccgacaaatc cgccaggctg gtcacgccca
     6541 tgcgcgcggc gaattcggtg gcggcccggg cggcggcgtg cttttccgca aaagtctttt
     6601 ccggcaccag gcgctccagg ccgtccgcgg cgctgtcctt gaggatgccg gtgggctcgc
     6661 cgctcccggg gtcgcggacg atcaggccgc cgggcgggtc gggggtttcg cgggtgatgc
     6721 ccgcccgctg gagggcgacg ctgttggcca gcgccatgtg gccatcgagg cggcggagca
     6781 gcaccggatg atcgggggtg gcggcgtcaa tcatctggcg ggtgggcagc ggcgcgccgg
     6841 gccatttctc atgatcccag ttgcccccca gaatccactg ccctttcggg acgttccgga
     6901 cgtattcccg cacgcgccgg gtcagttcct ccggggaggc ggcctcgcgc aggtcaatgc
     6961 tggcaagcga aaacccgccc atgaggaagt gggtgtgcga atcgttgaag ccgggcagca
     7021 ccagtttgcc gccggcgtca atgacgcggg ttcgggggcc ggcgagggcg cggatttcat
     7081 cggtcgtgcc cagggcggcg atgcgggagc cgcggatggc cacggcgtcg gcggtggggc
     7141 gggcggcgtc catggtgtgg acggcggcgt tgaggatgac gaggtcaggg gtggacatgg
     7201 ggttttgtgc ttggacggga gcagtcaaca tggttaacgg aagagcgaga gctgccaggc
     7261 gcgctttcat tgactgacgg tgcgattcat ctgatgggca aagcctacac cggcgggcgc
     7321 ggggttgtca cgccggaacc gcggcgctgg aaggcgtgcg gcttaattag aattcgcctt
     7381 gcggcgcgaa gcggcttagg ttcctgacca aaagacttat gtctgcacgc gacgtatcga
     7441 aagacccggt ctggcggggg gaggaactgg gccagccgat cccggccctg ccccacgcgg
     7501 tttcagtggc tctcccgcgc tgggaggacg tggtgggcta cgaagaaaag aagcccgagg
     7561 tggtcaacgc gctggccggc ggttatccgc gcttctttat ccacgcggaa gtgcgggaac
     7621 tggcgcgcca gcttggggat gggcggccat gcctgccttt tccctcgccc cgcgcggccg
     7681 ggctttgcgc ggagttcatc gagcgcacca gcggccagca ggccaaagtc ctgaccgggc
     7741 gcggtctttc cggcgtggtg accagcgacg cgggcagcgc cgccctgcgc gcgttctggc
     7801 agcatacggg gctgatcgtc tcctcccgcc aggccgcggc gcacctggcc gggcgcgcgg
     7861 ctggccccga agaactggcg gcgctccccg ccttgcggcg tcaactggcc ggattctacg
     7921 attgcgccga ggacgatgta tttctggcgc cgaccggcat ggccgcgcag ttcgccgcgc
     7981 ttcaggcggt ccggcagcgg acgcccgggc ggcccacggc ccagttggga tttccgtatg
     8041 tggacacttt aaagctgcaa cagaagctcg gccacggcgg catcctgctg catcgcctgg
     8101 gaagcatcga gcaggagctg cgcgacttgc tgagccggca gcccctggcg gcgtgcttct
     8161 ccgaaatccc cgggaacccg ttgctggggt cggccgatgt gcgccgtctg catcccctgc
     8221 tccgcgcgca acgcgtgccg ctggtggtgg atgatgtggt cgccaccccg gcgaatatag
     8281 acttgagcgg ctgcgccgac ctgatcgcca ccagcctgac caagttcatg gtcggcaccg
     8341 gcaacgcgat gggcggcgcg ctcatctgca acccccgctc gccgctgtac gccgaattga
     8401 aacccattat cagcgcccgg cacgaggagt tgctgtgggg cgaggatgcg gtcgtgctgg
     8461 cggcccagac gcggagtttc ccggagcgca tgaagcggca taaccagaac ggcctgtggc
     8521 tggccgagcg cctgcgccgg cacccggctg tcgaacgggt ctggtatccc aagtgggagt
     8581 tcgccgaggc ctacgaggcg gtgcgccgtc cggagggcgg ctggggcgcc ctgattacgt
     8641 tcctgcccaa agagggcgag acgaggtcgc ccggcattta cgaccggctc gaattctgca
     8701 agggccccag cctgggcacg gtcttcacgc tggcgtgccc gttcatgttg ctggctcact
     8761 acacggaatt ggagtgggct gaatcgtgcg gcgttccccg cagcctcatc cgtctttcag
     8821 tcggattgga ggatccggaa gacctttggc ggcggctgga gcgggctttg accgcccgct
     8881 aacccgtggt ccccatgctg cgcccgatgc ttcccttatt ctcccggcct tcggcaggcg
     8941 agcggtgcgg gcgcccaacg cccggcgctg gcgcgccccg gctgctgttg cttgccggcc
     9001 tggcgctggc aatcctggga gcgccggctt ggggcggcga tcagccacaa tgggggcagg
     9061 cttggacgcg gaacatggta tcagaagagc gcggcctgcc ggatacgttc gatccgcgga
     9121 ccggccgcaa cgtccaatgg tcggcggaac tgggcaccga aacccacgcc acccccatcg
     9181 ttgccggggg gcgcgtttat atcggcacca acaacggcca cccgcgagac ccggcccaac
     9241 aaggcgaccg gggcatcctg atgtgcttcg acgaacgcac cgggcgtttc ctctggcaga
     9301 tggttttcct gaagcgcgac gaggatattt actttgattg gccccgcagc ggcatcgcat
     9361 cgcccgtcac ggtcgaagat gggcgcgtgt atttcgtcaa caaccgtggc gaagtcatct
     9421 gcctcgaacc gccgccgatt ccggaaaagg cggaaggcgg cgcggccaaa accgaaacag
     9481 ccaacctgcc cgcgccgcgc attctctggc agttcgattt gacggcgggc gccgggattt
     9541 ggtctcacga cggcgctcac agcccgattc tcatcaacgg cgattatcta tatctcaaca
     9601 ccggcacggg cgtggacaac acccaccggc gtatccggac gccggacgcc ccgagcctgg
     9661 tcgtgctgga caagcgcact ggccgcctgg tggcgcgcga aaacgagggt atcgccccca
     9721 acatcttcca cgccacctgg gccgggcccg cgctggcggc catcaacggc cagcctcgcc
     9781 tgttcttcgc cgccggcaac ggcattgtgt atgccttcga aacgctgccg aaccgggggc
     9841 cggaaaaccg caaaacccgc accgcgcctg ccagtccttc gggctcgccg accggcgcgg
     9901 cggcgtcccg ttcgcctgct tttctccagc aagtctggcg cttcgacttc gaccccgcgg
     9961 cccccaaaac caatattcac ctctacgtca gcaaccggcg cgaaagcccg agcaccttct
    10021 atggcatgcc cgtttttcat cacaaccgcc tttacctggc tggcggcggc gatctctggt
    10081 ggggcaagca cgaagcctgg ctcaattgct tcgaggccac gggcaccggc gacatcactc
    10141 cccatgcgct ggcctggtcc tatccgctcc aaaagcacgt tctggccacc ccggccattt
    10201 ggcgcggcct ggtctttatt gctgattgcg ggcgcatgtt ccattgcgtg gatgcgcaga
    10261 ccggccagcc ctgctggacg cacgagctca aaggcgaagc ctgggcctcg cccctggtcg
    10321 cggatggcaa agtctatctg ggaacccgcg gcggcagttt ctacgtcttc gccgcggagc
    10381 gggataagaa ggtgctggcc accgtggccc tggagcgtcc catcagcgcc accgccaccg
    10441 cggccaatgg cgcactttac gtggccacca tggaccgcct ttatgccctg cgccaggggg
    10501 tcggcgcgca gtagcccgcc ccggcgcgga agccgcggcc gggggcggcg ggagggcgca
    10561 tcccggtttt agcgggcgcg gcgttcccgc ccgggccctg gcgaaggccg ctttggctcc
    10621 cctttcccgc ccggccgagg ctcccgtgac ggcgcgaaat cccttgcttt tctaccgggc
    10681 gttgtgaaat atgtccccca ctttatgggt catgtaaaac tgcatattcc cgggccggta
    10741 gagatcagcg aaaagaccta ccgcgctttc tgccagccga tgattggtca tcgcgggcaa
    10801 ggcttcaaag acctctacgc gaggattcaa ccgcaactcc agcaattgct ctacacccgg
    10861 caattggtct atctgagcac ctcctccgcc tggggcgtga tggaaggcgc cattcgcaac
    10921 ctggtctccc gcaaggtgct caactgcatg tgcggcgctt tttcggataa atggctggat
    10981 gtctccaggc gttgcggcaa ggacgccgag gggttgcagg tgccctgggg gtcgcccatt
    11041 cgcgccgcgg aggtggaccg caagctggcg accggccagt ttgacgccct gacggtcatt
    11101 cacaacgaga cctcaacggg cgtgatgagc cccctcgcgg aaatcgccgc gctcaagcag
    11161 aagtatccgg acgtgatgtt catcgtggac tcggtcagct cgatgaccgc ggtgccgctc
    11221 aagttcgacg aactgggcat tgacgtgctg ctggcaggca cgcaaaaggc tctggcgatg
    11281 ccgccggggc tggcgctttt tgtgtgttct ccggcggcgc tggccaaggc ggctacggtc
    11341 aaagaccgcg gctactattt cgatttcgtg gagttccaga agaacgccgc gcagaatatg
    11401 acccccagca cgccgagcat cagccacatt tacgcgctgg aatccaagct gcaggacatc
    11461 tttgccgagg ggttggaggc gcgttatgcg cggcatcgcc gcaccaacca gctcacccgc
    11521 gactgggcgg cccggcacgg attcacgctc ttccccgacc cgggcttcga gtcggtcacg
    11581 ctgacctgcg tcaacaacgg cgcgcgcccc ggcgggcggg tggtggatgc ggagaagctg
    11641 gtgaaactgg tcaaggacca gggcttcctc atcaacggcg gctacggcaa aattaagggc
    11701 accaccttcc gcgtctcgaa catgggcgat gagacggaag cgaccatgaa ccagctcttc
    11761 gccgccatgg ataacgcgat gaagcagttg tgatcgccgt ctggctgggc gggctgcgcg
    11821 gggcggtgct ccgtgcgggg ttgatcctgt ccctgcccgc cttggcggcc gatctctacg
    11881 tctcgccggg gactggccgg gactccaatc ccggcacgcg agcccggccc ttcgccacgc
    11941 tggaacgcgc ccgcgacgaa gtccgccgtt tgcggcaaga ccgcagttcc ggcggcggcc
    12001 ctttcaccat ctggcttgag ggcggcgatt acctccgcac taacgcgctg gaactctccg
    12061 ccgccgactc cggcacggcc cgggctccgg tcacctggcg cgcccgcaaa ggccaggccg
    12121 tccggttgat gggcgggcgc aagctgtccg gatttgcgcc ggtcaccgac cccgccatcc
    12181 tttcccgcct gtcccccaat gcccgcacca acgtcgtgca ggccgatctc cgggccctgg
    12241 gcatcacaga cttcggccag atgcagtccc ggggattcag ccggccattg gcccccgcgc
    12301 acggcgagct gttttacgac ggccgcccca tgaccctggc ccgctggccc aacgtaggtc
    12361 attggaaaca cctggccggc taccccgaat ccggcgcgat caaaaacgaa tggggccgcg
    12421 aagtgggacg gctggaggac gggttcacct ataccggcga ccggccccgc cgctgggcgg
    12481 acctcagcgg cctctgggtg catggctatt gggcgtggga ctgggccaac tcctacgagc
    12541 gggttgccgc gattgaccgc gatcgccgcc tcatccggac ggccgcgccg tacggccttt
    12601 acagcttccg caaaggccag cggttctatt tcctcaacgt gctggaggaa ctcgatcagc
    12661 cgggagaatg gttcctggac cacggcaccg gccggctcta tttctggtcg cccgctcccc
    12721 tgcccctccc gggcgtggaa gcgcgccggg ctccaggcgc aaagcccagg ccttccgatt
    12781 cgcccgccgc cccggaaatc ctgctctcgg tcctcgccga gccactgctc aggctgacgg
    12841 acgcctcgca catcatcttc cgcggcctga ccctcgaagc cacccgcggc aacgcggtgg
    12901 aaatacgggg cggcgccagc aaccgcatcg ccggctgtct catccgcaac atcggaaact
    12961 atggcgtcac gatttccggc ggcgccgggc atggggttca gtcatgcgac ctcttcgaca
    13021 ccggcgatgg cggggtgatg ctcgacggcg gcgaccggca gactcttcgc cccggcggcc
    13081 atttcgtcga gaactgccat tttgcgcgcc agggccggtg gtccaagtgc tacgtccccg
    13141 cagttctcat cagcggcgtc ggccagcgcg ccgcccacaa cctcatccac gatcatcccc
    13201 attgcgccct ccttttcaac ggcaacgacc actgtctcga gttcaacgag attcatcata
    13261 tcgccctgga aacgggcgat gtcggcgcca tctacaccgg gcgggattat accagccgcg
    13321 gcaatcgcat ccgccacaac ttcatccatg acaccggcgg cgtcggcatg ggctcgatgg
    13381 gcgtttatat ggacgactgc gtcagcggcg ccgggatcgt cggcaatatc ttctaccggg
    13441 tccggcgcgc cgttttcctg ggcggcggcc gtgaccacca ggtcctcaac aatatcttcg
    13501 tggattgcaa ctacgccgtc gaactggacg gccgcggcct ggatgcctcc ccggtctggc
    13561 gcaacatggt ggacaacacg atgcgccatc gcctgaccga agtcccgctc tccctttacc
    13621 gcgagcggta tcccgccctg cagacgctcg attcctacta cggcccgccc ggcggtcccg
    13681 cccttgccgg ccccaacttc cagggcgtgc cgcccgagca taacgtcgtg gcccgcaacg
    13741 tctgcgtcgg caaatggctc catgcctact ggcacgcctc gcccgagatg atccaattgg
    13801 agaacaatct caccaatgcc gccgccgcct tccgccgccc gcccggcaac ccgccccagg
    13861 cccgggactt cgccttgcgc cgcaattccc ccgcccggga cctcggcttc cagcctcttc
    13921 cgctcgacca aatcggcctc taccgcgata aactccgcgc ccgcctcccc gctccgctct
    13981 caagcaaaac gcgccgctaa ccacctgcga gcgggctttg gggggcaagg ggaaactcga
    14041 ctcccggccg accatcccga atccctccgg ctcaagctaa ttagagtaca ttaataaaac
    14101 tgtgccgcaa tgggcaaacc agtttgttgc atcctgacgt gggatagtct tcaaagctga
    14161 ggctattgcc ccgatccgtt ccgagtcgct gcgtgcctct tggcctagcc cgaatatttc
    14221 atcagcgtag ggcgcggcgg atttgagggg caaaatggaa acgggatggg gcggaagccc
    14281 tcaaaatgct gattatttcc ctgcgtgggc acagtccccc ttacccccgt ggttggccag
    14341 cgtgccgggg acagcactcc cggcccttca accactgccc aaggcgcgga acacctggcc
    14401 gaagattttc tgatacggat aggcgctgga gagcagcagt t
//
LOCUS       DAOPQG010000091        14237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5011_length_14237_cov_15.945142, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000091 DAOPQG010000000
VERSION     DAOPQG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_14237_cov_15.945142"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            686..1957
                     /locus_tag="PKI07_10355"
     CDS             686..1957
                     /locus_tag="PKI07_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC51492.1"
                     /translation="MSRLPFELLLALRYLRPKRTFVSVITLISVLGVTLGVAVLIIVI
                     SVMSGFDRKMRDSIIGFNPHLRILPRDETLKDYAAVARTVAAHPQVKAVAPYIEGPVL
                     VQTEPAHGQARIGTPYLRGILPQIETNLNVLPHRIIEGEFDVGDRGLLIGSAFAYNMQ
                     LAVGDRVEIGSPSMLARWRESSRKEQASAPILPEYEVRGIFDVGYNEYNLSVIIASLA
                     DAQDLYDLPDSVHGLVVMLHDPDQAPAVEPELAAALGPRYLLRSWLEENATLLNALLV
                     EKNVMFYLLFFIMIVAALCILSALITFVVQKTREIGMLKALGATDAQVSLIFLSQAAL
                     VGALGDLAGYGLGMLALHYRNEFLHFMNRLTGFELFPASIYGFTELPALIFPHDILLI
                     CGSAWFICLLGGVIPAWRAGRLQPVEALRYE"
     gene            1950..2636
                     /locus_tag="PKI07_10360"
     CDS             1950..2636
                     /locus_tag="PKI07_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC51493.1"
                     /translation="MNEPLLRARGLHKTYTLGRRSLEVLRGVDLELRRGEFLALQGAS
                     GAGKSTLLHLLGGLDTPTRGEIELAGRNLAALRPRDLARLRRQEVGFIFQAYHLLPEL
                     DALENVCLPARMARVPAAQAAARGRELLARVGLAARLEHKPYELSGGEQQRVAIARAL
                     INQPPLLLADEPTGNLDSRTGGEIIELLCALRNEQQTTLIMATHDPRVAARAPRIVEL
                     VDGRIANGAV"
     gene            3050..4414
                     /locus_tag="PKI07_10365"
     CDS             3050..4414
                     /locus_tag="PKI07_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51494.1"
                     /translation="MNATPSAAALPRRDFLKKTAAVTAAAAAAPLLRAPLYGQAPSAN
                     VAGANNRIQVAVIGVGFGIGQNHLTGIHEKANENNTVITAACDVFNKRRDFAKAKANL
                     KDADVYVDYRKMLERKDIDAVLIATHDPLHAQMILDSLETGRHVYCEKPLTRYLGEAF
                     QVYDKVKSSGKVFQVGSQGCSAGGYQKCAELIKDGKIGPLVWGQAWYSRNSLTGEWNY
                     LIENETRPENIDWERWLGPVKQRDPFSAEQYHRWRKYYRYCAGPLGDLAPHKLHPVLL
                     ATGHPEFPVRVVSIGTSNVHIDKNVPGTPVREIPEHAQLLAEFPSGFVLMVTCCTVNA
                     STPGTSLYGHKANLEIDATGGKVNLLPQREFGDEIDPENFANLQPEEIRVHEKNWFDC
                     IRSGKQPNADIELAIRVQTVISLAEMSDRLKVACLFDEKTRKVTTGSGTEVTPLTYGT
                     LPQS"
     gene            4510..5475
                     /locus_tag="PKI07_10370"
     CDS             4510..5475
                     /locus_tag="PKI07_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOC51495.1"
                     /translation="MPAVTLACHAMATRFELVLHGDHAPALRAAGEEALRLVEQLEGR
                     LSLFRPASEIARVNAGAARAPVRVSPEVFALLQRAQKLSAETGGAFDLSVAPLVRCWG
                     FMGGDGCLPSPAAVAEARARVGMHRVQLDPGAYTVRFAADGVMLDLGAIGKGYAIEQG
                     AEVLREAGVTSALFHGGTSTVQAIGAPPGEASWQVALEVPSPAPGTPPRLLATVPLRD
                     EAMSVSAVCGNHFQAGGRTMGHILDPRTGEPAQGTVLTAVVLPSATETDALSTALLVL
                     GAAGQETLAGLRPGLRALVVSESGGRLSVAARGITPRALAGQNPV"
     gene            5515..6402
                     /locus_tag="PKI07_10375"
     CDS             5515..6402
                     /locus_tag="PKI07_10375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOC51496.1"
                     /translation="MLEDRHYLRQPWLRSSGAMTVALLVVNVTAFVLQNLVYRFTHFP
                     LDGWFALSVAGLRQGFVWQLLTYQFMHGGWLHLLLNGWAIYVFGRAIEETLGGRSFLT
                     LYFASGVAGGLFQALFGVLLHGVFAAPVVGASAGAFGLVAAFATLYPERTLTLLLFFV
                     LPLNLRAKYLLLFSALLAVLGILVPTDNIAHAAHLGGMVMGLVYVRYAIHGQWRWPRL
                     RRPGAPPVRLLARPRRPYPPEARPARPADSDEDLPPAEFLSQAVDPILDKISAHGIQS
                     LTERERRILETARAKMARR"
     gene            6489..7802
                     /gene="purB"
                     /locus_tag="PKI07_10380"
     CDS             6489..7802
                     /gene="purB"
                     /locus_tag="PKI07_10380"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOC51497.1"
                     /translation="MIQRYARPAMREIWSEQRKLEIWLRIELLASEALCAAGLVPRRD
                     LAQMKRRAAFRLERCRELERTLNHDVIAFTTNVAEQIGAPASRWLHFGLTSSDILDTA
                     LAVQMAQAADLLIADVKALRRVVAARAKKYQFTPMIGRSHGIHAEPITFGLKLALMYD
                     EFGRALRRLETARATAATGKLSGAVGTSAHLAPRVEALVCRKLGLRPASLATQVVQRD
                     RHAEFIGALALVGASVERWATEFRHLQRTEVLEVEEFFAPGQKGSSAMPHKRNPITGE
                     RLSGLARVLRGHALAALENVALWHERDISHSSVERIIFPDSCALLDYMLVTLTRLVRG
                     LVVYPANMRRNLDLSLGLWNSQTVLLALIRKGLDRERAYELVQRNAMRTWQAKHAGRP
                     DADFLRQLQSDPDVARHFQRGELESLCSLDFHFRQVKYRFRKLGL"
     gene            7944..8420
                     /gene="tnpA"
                     /locus_tag="PKI07_10385"
     CDS             7944..8420
                     /gene="tnpA"
                     /locus_tag="PKI07_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOC51498.1"
                     /translation="MANPYSQIYIQVVFAVQYRQYLLLPEFREEVQKYITGVTTERGQ
                     KLLAVYCMPDHTHLLVGLKPAIALSDLVRDIKNASSNFINSKGWVLGRFCWQEGFGAF
                     SYAHSQISDVIRYIQTQPQHHARRSFREEYLQFLKRFEIDHDERFILKDVYQTDPP"
     assembly_gap    8591..8690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8703..9413
                     /locus_tag="PKI07_10390"
     CDS             8703..9413
                     /locus_tag="PKI07_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51499.1"
                     /translation="MSSSTTSCKFLLLASLCLAPLAPGAAQTWQSALSRMPLPAGVPQ
                     LNRTNCVETLLRSFQSNDVVKALIFMPGATDELYFFRRARANLPAPPVSLLAAVTALT
                     NQTLIRATFRPPLLLLHTAEDLLEPAVTLKHPSAVERLRQARFAPHVLYLDRDWDCLQ
                     PLLSSALAIDLRPQRYSRDSWHFYRHSFAAWNLTGWEALEATALAGQTRFTVRRRQVV
                     FELDMRPRVAPRVESYPP"
     gene            complement(9401..10198)
                     /gene="sufC"
                     /locus_tag="PKI07_10395"
     CDS             complement(9401..10198)
                     /gene="sufC"
                     /locus_tag="PKI07_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOC51500.1"
                     /translation="MLEIQDLTAGVEGKPILKGINLRIYPGEVHAIMGPNGSGKSTLA
                     AVLAGRDGYEITAGRVAYLGRDLLELAPEDRAREGLFLAFQYPVEIPGVNSTYFLKAA
                     LNEIRKHRGQPELDAMEFLTLVKDKIQLLELNEDLLRRSVNEGFSGGEKKRNEVFQMA
                     VLEPRLAVLDETDSGLDIDALKIVSNGVNKLRRPDNAQLVITHYQRLLDYIIPDYVHV
                     LADGRIVRTGDRKLALELEEKGYDWLLKPASAAAGTGGPAAGAAQGG"
     gene            complement(10245..11693)
                     /gene="sufB"
                     /locus_tag="PKI07_10400"
     CDS             complement(10245..11693)
                     /gene="sufB"
                     /locus_tag="PKI07_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOC51501.1"
                     /translation="MSTATETIAGLVRKEYKYGFVTDVETESAPPGLNEDIVRFISAK
                     KREPEFLLEWRLRAYRHWLTMREPNWAHVHYPPIDYQDSVYYSAPKQKGDGPRSLEEV
                     DPKLLETYEKLGIPLKERARLAGVAVDAVFDSVSVGTTFKKQLAEKGIIFCSFSEAVQ
                     EHPALVRKYLGTVVPYTDNFFAALNSAVFSDGSFCFIPKGVRCPMELSTYFRINAANT
                     GQFERTLIVAEEGASVSYLEGCTAPMRDEHQLHAAVVELIALDQAQIKYSTVQNWYPG
                     DEEGRGGIYNFVTKRGLCKGKHSKISWTQVETGSAITWKYPSCILLGDHSTGEFYSVA
                     VTNNYQQADTGTKMVHVGRNTRSTIVSKGISAGRGQNCYRGLVKVQKSAAHARNFSQC
                     DSLLLGDRCGAHTFPYLEVRNATARVEHEASTSKIGEDQIFYCNQRGLATQDAVTLIV
                     NGFCKEVLRELPMEFAVEAQKLLGVSLEGSVG"
     gene            complement(11762..12226)
                     /locus_tag="PKI07_10405"
     CDS             complement(11762..12226)
                     /locus_tag="PKI07_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOC51502.1"
                     /translation="MVEAAMDLRDELRVDERLARRGFRFTPQRQQVYAVLLQQRDHPT
                     AERVFIRAKRKMPEISMATVYNCLDALVKCEVARLVALERGAARFCPNMRPHGHFYCT
                     VCERVFDVDLPAAPGIALPPGCRVERYELAIHGRCAACAPARPGRRGKRRSG"
     gene            complement(12430..>14237)
                     /locus_tag="PKI07_10410"
     CDS             complement(12430..>14237)
                     /locus_tag="PKI07_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51503.1"
                     /translation="GCYLFALGFFALGLMSKPMLVTVPLVLLLLDYWPLERWREREGG
                     GAASAPGGGSPVSLAAPVLTRLLWEKVPFAVLSAASCVVTFLVQRKAGAVHSLETIPL
                     EFRITNALISYVRYAGKLVWPAKLAVFYPAPAEWPPLWVALAALGLVGVSVLVVRAAR
                     RAPWLAFGWFWYGVMLIPVIGLVQVGQQAMADRYTYLPLIGLCVAGVWSGAELVRRRA
                     RAAVALAAAGAVALLAAAGALTWRQAGFWRDSTSLFEHALAVTRENFVAHNNLGVVLL
                     DADQQAAALRHFTKAVRIKSNYPEGLGNLALCRLKEGRTDEAEELLRRSLQKRETVET
                     WHNLGRMEEQRGRAAEAEQAYTKALRLRPNHAPSLMALGILLSGQHREEEALRYLQAA
                     VRAAPENADARFNLAFALNNRGEFAGAAAQYAEVCRLHPEDVEARQNLALALLGANQP
                     AAAAAQFRRALELRPAAHLHHYLALVLDSQGQAAEALAHYRDAARLGPNTVLYLNDLA
                     WFLATTARPDLRDGAEAVRLAERARELNGGREARIWGTLDAAYAEAGRFDEALKAAAR
                     ARELAQAAGQADIARQAEERMALYQRGQPYHMPPP"
BASE COUNT         2247 a         4992 c         4410 g         2488 t
ORIGIN      
        1 acgccaaaag gggattggta gtatgtttcc gcgcccagac tgcccgtgcg cggagcagat
       61 agtattttca taatgtctgt cctctctaat ggttatttta attgtttgtc tattacgggc
      121 cggagagcga ctccgcttcc tgccgttccc tgggctggga gttccagccg cccccacccc
      181 aagggtctgg aggaaggctg gcgggcaggg ggtcttcgtg caagggcaat tttttggggt
      241 tggtggtggt gggcgggtgc ggagcgccgc gtgtgggcac gcggcctaca gggggcggga
      301 tgatgcgggg gggcctgtgg gttgtcggga gtgcggtgct gcgagagggc atgggggctg
      361 ccgcggcgtg cttccatttc ctgcaggccg cgtgggggca ggcggcgctc gggggccgtt
      421 tgtctggcgg ttgagaaaaa catttgcgcg cccgcccgga ttgtctgaag gaaagcccgc
      481 cggcttggga gggcaatctc attcccgcta tcctcaacag cccgggcccc gggcgtgcgg
      541 ccaggcaccc gctccaaagc gcttaaccgc cgtcccgccc gccgccccac cctccccggc
      601 ttctgatctg caaaaccaaa ggccccgcga cccggggcgc ccgccgcgtt gacacgcccc
      661 gcccggccct ctagcttcag cccgcatgtc tcggttgccg tttgaattgc tgctggcgct
      721 gcggtatctg cgacccaagc gaaccttcgt ctcggtgatc acgctgattt ccgtgctggg
      781 ggtcacgctg ggcgtcgcgg tgctgatcat cgtcatcagc gttatgagcg ggttcgaccg
      841 gaaaatgcgc gactcgatta tcgggttcaa tccgcatctg cgaatcctcc cgcgcgacga
      901 aaccctcaag gactatgccg ccgtcgcccg cacggtggcc gcccaccccc aggtcaaggc
      961 ggtggcgccg tacatcgaag gccccgtgct ggtgcagacc gagcccgcgc acggccaggc
     1021 ccggatcggc accccctacc tccgcggcat ccttccccaa atcgagacga acctgaacgt
     1081 gctgccccat cgcatcatcg agggcgaatt cgatgtgggc gaccgggggc tgctgatcgg
     1141 gagcgcgttt gcgtacaaca tgcaactggc cgtcggcgac cgggtcgaga tcgggtcgcc
     1201 cagcatgctg gcccgatggc gggagagcag ccggaaggag caagccagcg cccccatcct
     1261 gccggaatac gaagtgcggg gcatcttcga cgttggctat aacgaataca acctgtcggt
     1321 catcattgct tcgctggccg acgcgcagga tttgtatgac ctgccggaca gtgtgcatgg
     1381 cctggtggtg atgctgcacg acccggacca ggcgcccgcg gtcgagccgg agctggccgc
     1441 cgcgctcggc ccgcggtatc tgctccggag ctggctggag gaaaacgcga ccctgctcaa
     1501 cgcgctgctc gtcgagaaaa acgtcatgtt ctacctgctg ttcttcatca tgatcgtggc
     1561 ggcgctgtgc atcctcagcg cgctgatcac gttcgtcgtg cagaaaacgc gcgagatcgg
     1621 catgctcaag gcgctgggcg ccaccgatgc gcaggtcagc ctcatcttcc tgagccaggc
     1681 cgcgctggtg ggcgccctgg gcgatttggc gggctatggt ttgggcatgc tggcgctgca
     1741 ttaccgcaat gaattcctgc acttcatgaa ccgcctgacc ggcttcgagc tgttccccgc
     1801 ttccatctac ggcttcaccg agctgcccgc gctgatcttc ccgcacgaca tcctgctcat
     1861 ttgcggcagc gcctggttca tctgcctgct gggcggcgtg attccggcct ggcgcgccgg
     1921 ccggctccaa cccgtggagg ctttgcgcta tgaatgagcc gctgctgcgc gcgcgcgggt
     1981 tgcataaaac ctacacgctg ggccggcgct cgctcgaggt tttgcgcggc gtggacctgg
     2041 agctgcggcg cggcgaattc ctggccctgc agggcgcttc cggcgccggc aaaagcacgc
     2101 tgctccatct gctcggcggg ctggacaccc ccacccgcgg agagattgaa ctggccgggc
     2161 gcaacctcgc cgcgctccgg ccccgggatt tggcgcggct ccgccgccag gaagtcggct
     2221 tcatcttcca ggcgtatcac ctgctgccgg aactggacgc gctggaaaac gtctgcctgc
     2281 ccgcgcgcat ggcccgcgtc ccggcggcgc aggccgcggc ccgcgggcgc gagctgctgg
     2341 cgcgcgtcgg cctggccgcg cggctcgaac acaagcccta cgagctttcc ggcggcgaac
     2401 agcagcgcgt ggccatcgcc cgcgcgctga tcaatcagcc tcccctcctc ctcgccgacg
     2461 agcccaccgg caacctcgac tcgcgcaccg ggggggagat catcgagctg ctctgcgccc
     2521 tccgcaacga acagcagacc acgctcatca tggccaccca tgatccccgc gtggccgccc
     2581 gcgctccccg gattgtggag ttggtggacg ggcggattgc caacggcgcg gtctaagccg
     2641 cggacccgcg ccagaccggc agggacggaa cgctcaggcc gggaggcttg agcggtcaac
     2701 gcggcctttc ggcccggcaa agcggcccgt tttcgggcat tcccagtccg ggctggcccg
     2761 ggccgggtcg agccaggctc catccccgtt ccaacgctcc ccgggatttc ttcgtcccca
     2821 cgctgcggcg gtctttgcgg gaacgccgtg cgggaaacgg cggggctgcg ggagccgagg
     2881 aaaggggcgc ggagagctgg catggattgg gagaaacacg gcgagttggg gctgatgcgt
     2941 ttgcgcccgg gctggagtgg tttgcggtat tgaccctgaa tgtctttgcg gtaccttacg
     3001 tccacttact gttgtgacta ataacgctct gacaaatcat tctatggata tgaacgccac
     3061 tccgtccgcc gccgcgctgc cgcgccggga ttttctcaag aagactgccg ccgtcacggc
     3121 cgccgccgcc gccgcgcccc tgctgcgcgc gcccctctat ggccaggcgc cttcggccaa
     3181 cgtcgccggg gccaataacc gcatccaggt tgccgtcatc ggggttggtt ttggcatcgg
     3241 gcagaaccac ctgaccggca ttcacgaaaa ggccaacgag aataacacgg tcattaccgc
     3301 cgcctgcgac gtgttcaaca agcgccggga ttttgccaaa gccaaggcca acctgaagga
     3361 cgcggatgtc tatgtggact accgcaagat gctcgagcgc aaggatattg acgccgttct
     3421 gattgccacg catgacccgc tgcacgcgca gatgattctg gattcgctgg agaccgggcg
     3481 gcacgtctat tgcgagaaac cgctcacccg gtatctgggg gaggcttttc aggtgtatga
     3541 caaggtgaag agcagcggca aggtgttcca ggtgggctcg cagggttgct cggccggggg
     3601 ctatcaaaag tgcgccgagc tgatcaagga cggcaagatt ggcccgctgg tctggggcca
     3661 ggcctggtat tcgcgcaaca gcctgaccgg cgaatggaat tacctgatcg agaatgaaac
     3721 ccggccggag aacatcgatt gggagcgctg gctggggccc gtcaaacagc gcgatccctt
     3781 cagcgccgag cagtatcacc ggtggcgcaa gtactaccgc tactgcgccg gcccgctggg
     3841 cgacctggcc ccgcataaat tgcatcccgt cctgctggcc accggccacc cggagtttcc
     3901 cgtccgcgtg gtttccattg gcaccagcaa cgttcacatt gacaagaacg tgcccgggac
     3961 ccccgtgcgg gagattcccg agcacgcgca attgctggcc gagttcccca gcggttttgt
     4021 gctgatggtg acgtgctgca ccgtgaacgc ctcgacgccg ggcaccagcc tttacggcca
     4081 caaggccaat ctcgaaattg atgccacggg cggcaaggtc aacctcctgc cgcagcgcga
     4141 gtttggcgat gagattgacc ccgagaactt cgccaatctg cagcccgagg aaatccgggt
     4201 tcacgagaag aactggtttg attgcatccg ttccggcaag cagcccaacg cggacatcga
     4261 gctggccatc cgggtgcaga cggtgatctc gctggcggaa atgtccgacc gcctgaaggt
     4321 ggcctgcctg ttcgacgaga agacccgcaa ggtgaccacg ggcagcggca cggaagtgac
     4381 gcccctgacc tacggcacgc tgccgcagtc gtaagcggcc gctccccgga ggggtggcgc
     4441 cgcggaggcc ggaagggcgg cgcgccgcat cccttcccgc gggagcgccc ccgctccctt
     4501 gctgttccca tgcccgccgt cacgctcgcc tgccacgcca tggcgacgcg gttcgagctg
     4561 gtgctgcatg gcgaccacgc cccggcgctg cgggcggcgg gcgaagaagc cctgcggctg
     4621 gtggagcagc tcgaggggcg gctcagcctg ttccgcccgg ccagtgagat tgcccgcgtc
     4681 aatgccggcg ccgcgcgcgc gccggtgcgg gtttcgccgg aggtgtttgc cctgctgcag
     4741 cgcgcgcaga agctgagcgc cgagaccggg ggcgcgtttg acctttcggt ggccccgctg
     4801 gtgcgctgct gggggttcat gggcggcgat ggctgcctgc cgtcgccggc ggcggtggcg
     4861 gaggcccgcg cccgggtggg gatgcaccgg gtgcagttgg atcccggcgc gtatacggtc
     4921 cgattcgccg cggacggggt gatgctggac ctgggggcga tcggcaaggg ctacgccatc
     4981 gagcaggggg cggaggtgct gcgcgaggcg ggcgtcacca gcgccttgtt tcatggcggc
     5041 accagcaccg tgcaggcgat tggcgctccc ccgggggagg cgagctggca ggtggccctc
     5101 gaagtgccgt cgcccgcgcc gggcacgccg ccccggctgc tggccaccgt gccgttgcga
     5161 gacgaggcga tgtcggtgtc ggcggtttgt ggaaaccatt ttcaggcggg cgggcggacg
     5221 atggggcata ttctcgatcc gcgcacgggc gagccggccc agggcaccgt cctgaccgcg
     5281 gttgtcctgc cctccgccac ggaaacggat gcgttgtcca ccgccttgct ggttctcggc
     5341 gcggccgggc aggaaacgct cgccgggctt cgccccggtc tgcgcgcgct ggtggtgagc
     5401 gagtccggcg ggcggttgag cgtcgccgcg cggggcatca cgccgcgggc cttggcgggc
     5461 caaaaccccg tttgaaaaac ccgcccgcaa cggctaggtt caacgcgaga gaatatgctg
     5521 gaagaccgtc attacctgcg acagccctgg cttcgctcct ccggggcgat gacggtggcg
     5581 ttgctggtgg tcaacgtgac ggcgttcgtt ctgcagaacc tggtctatcg cttcacccac
     5641 ttccccctcg acggctggtt tgccctgagc gtcgcggggc tgcggcaggg gtttgtgtgg
     5701 caactgctca cctaccagtt catgcacggg ggctggctgc acctgctgct caacggctgg
     5761 gccatctacg tctttggccg ggccatcgaa gagaccctgg gcgggcggag cttcctgacg
     5821 ttgtatttcg ccagcggcgt ggcgggcggc ttgttccagg ccctgtttgg ggtgctgctg
     5881 cacggtgtct ttgcggcgcc ggtggtgggg gcttcggcgg gcgcttttgg cctggtggcc
     5941 gcttttgcca cgctctaccc ggagcggacg ctgacgctgc tgctgttttt tgtgctgccg
     6001 ctcaacctgc gggccaagta cctgctgctg tttagcgcgc tgctggcggt gttggggatc
     6061 ctggtgccga cggataatat tgcccacgcc gcccaccttg gcggcatggt gatgggcctg
     6121 gtgtatgtgc gttatgccat tcatgggcag tggcgctggc cgcggctccg gcgcccgggc
     6181 gctcccccgg tgcgcctgct ggcccggccc cggcgcccat acccgcccga ggcccggccc
     6241 gcccgcccgg cggattccga cgaggatctg ccgccggcgg agtttctgag ccaagccgtg
     6301 gaccccatcc tggacaaaat ctccgcccac ggcatccaga gccttacgga gcgcgagcgc
     6361 cgcattttgg agaccgcgcg cgcgaagatg gcgcggcggt aggcgggacg cccggggcgg
     6421 agtgcggggc gcgcgccccg cgtccgcccg gctcgacaac ccgtctctcc ccgcctacac
     6481 ttccccccat gattcaacgt tacgcgcgcc cggcgatgcg ggagatctgg agcgagcagc
     6541 gcaagctgga gatatggctg cggatcgagc tgctggccag cgaggcgctc tgcgccgcgg
     6601 ggctggtgcc ccggcgggac ctggcccaaa tgaaacggcg ggccgccttc cggctggaac
     6661 gttgccggga actggagcgc acccttaacc acgacgtcat cgcctttacc accaacgtgg
     6721 ccgagcagat cggcgcgccg gccagccgct ggcttcactt tggcctgacc agcagcgata
     6781 tcctcgacac cgccctggcc gtgcagatgg cgcaggcggc ggacctgttg atcgccgacg
     6841 tcaaggcgct gcgccgggtg gtggcggcgc gggcgaagaa ataccagttt acccccatga
     6901 tcggccgcag ccacggcatc cacgccgagc cgatcacctt tggcctgaaa ctggcgttga
     6961 tgtatgacga gttcgggcgc gccctgcggc ggctcgaaac ggcgcgggcg acggcggcga
     7021 ccggcaagct ctccggcgcg gtgggcacca gcgcccatct cgccccgcgc gtcgaggcgt
     7081 tggtatgccg gaagctcggc ctgcgccccg cgtccctggc cacccaggtg gtgcagcgcg
     7141 accggcatgc cgagttcatc ggcgcgctgg cgctggtcgg cgccagcgtc gagcgctggg
     7201 ccacggaatt ccgccacctg cagcgcaccg aggtgctcga ggtcgaggag ttcttcgcgc
     7261 cgggccagaa aggctccagc gccatgccgc acaagcgcaa ccccatcacc ggcgaacggc
     7321 tcagcggcct ggcgcgcgtg ctgcgcggcc atgccctcgc cgccctcgaa aacgtcgccc
     7381 tctggcacga gcgcgacatc agccacagct cggtcgagcg catcattttc cccgattcct
     7441 gcgcgctgct ggattacatg ctcgtcaccc tgacccgcct ggtgcggggg ctggtggttt
     7501 atcccgccaa catgcggcgc aacctggacc tctcgctcgg cttgtggaac tcgcaaaccg
     7561 tgctgctggc gctcatccgc aagggcctgg atcgcgagcg ggcctacgaa ctggtgcagc
     7621 gcaacgccat gcgcacctgg caggccaaac atgccggccg ccccgacgcc gacttcctgc
     7681 gccagcttca atccgacccc gacgtggccc ggcacttcca gcgcggcgaa ctggagagcc
     7741 tgtgctcgct ggacttccat ttccggcagg tgaaataccg cttccggaag ctgggattgt
     7801 gaagcgcacc ggtggcgccc cgcccaaccc cgtcaggggt ggcctgtcta tagcaatgtg
     7861 ccaccattac gtcatttccc caccccctca ggggtgactc gtttattgtt gccacacccc
     7921 cctgtgcgcc ccatgcttct tccatggcga acccctattc tcaaatctac atccaggtag
     7981 tcttcgccgt ccaataccgc cagtaccttc ttctacctga gttcagggaa gaggtccaga
     8041 aatacatcac cggcgttacc accgaacgcg gccagaagct gctggccgtc tattgcatgc
     8101 ctgaccacac gcacctgctg gttggcctca agccagcgat agccctttcg gacctcgtgc
     8161 gcgacatcaa gaacgcttcc tccaacttca tcaatagcaa aggctgggtc ttgggccgtt
     8221 tttgctggca agaaggtttc ggcgcctttt cctatgcgca ctcgcaaatc tcggatgtta
     8281 tccgctacat ccaaacccaa ccccaacacc acgccaggcg ctcctttcgc gaagagtacc
     8341 tgcagttcct gaaacgattc gagattgacc acgatgagcg gttcattctc aaggacgtct
     8401 atcaaaccga ccccccctga cggggtttcg gaatgacgta atggttgtgc atttcgtttc
     8461 tacagaccgg ccacccctaa cggggtttga gggatgcgcc cgccaacgag aatcaccggc
     8521 tacagattgg tggtctcggc ctctccttgg acttctattt gcgagccaat aaccccgtta
     8581 ggggtgacct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taggggtgac
     8701 ctatgtccag ttcaaccacc tcctgcaaat tcctgctcct ggcctcgctc tgcctggcac
     8761 cgcttgcccc gggcgcggca cagacctggc aaagcgcgct cagccggatg ccgctgccgg
     8821 ccggggtccc gcagctcaac cgcaccaact gcgtcgagac cctgctgcgc agcttccagt
     8881 ccaacgacgt ggtcaaagcc ctgatcttca tgcccggcgc caccgacgag ctttacttct
     8941 tccgccgcgc ccgggcgaat ctccccgcgc cccccgtctc cctgctggcc gccgtcactg
     9001 cgctgaccaa ccagaccctg atccgcgcca cctttcggcc gccgctgctg ctgctgcaca
     9061 cggccgagga cttattggaa ccggcggtca ccctcaagca tccgtccgcg gttgaacgcc
     9121 tccggcaggc ccggtttgcg ccccatgtcc tctacctcga ccgcgattgg gattgcctgc
     9181 agccgctcct gtcctccgcg ctcgcgattg acctgcgccc gcagcggtat tcccgcgatt
     9241 cctggcactt ttaccggcat agttttgccg cctggaacct gaccgggtgg gaggcgctcg
     9301 aagccaccgc gctggccggt caaacccgct tcacggtccg gcgccgccag gtggtttttg
     9361 aactggatat gcgcccccgc gtggcgcccc gggtggagtc ctatccgccc tgagccgctc
     9421 ccgcggccgg gccgcccgtt cccgccgccg cggaggccgg cttgagaagc cagtcgtagc
     9481 ctttctcctc cagctccagc gccagcttgc gatcgccggt gcgcacgatt cggccatcgg
     9541 ccagcacgtg cacatagtcc gggatgatat aatccagcaa ccgctggtaa tgggtgatga
     9601 ccagttgggc gttgtccggg cgccgcagct tgttgacccc gttggacacg atcttcagcg
     9661 cgtcaatatc caggcccgaa tcggtctcgt ccagcaccgc caggcgcggc tccagcaccg
     9721 ccatctggaa cacttcgttg cgctttttct cgccgcccga gaatccctcg ttcaccgacc
     9781 gccgcagcaa atcctcgttc agctccagca gttgaatctt gtcctttacc agcgtcagga
     9841 actccatggc gtccagctcg ggctggccgc ggtgcttgcg aatctcgttc agcgccgctt
     9901 tgaggaagta ggtgctgttc accccgggga tttccaccgg gtattgaaac gcgagaaaca
     9961 ggccttcccg cgcccggtcc tccggggcca gttcgagcaa atcgcgcccc aggtacgcca
    10021 cccgtcccgc cgtgatttcg tagccgtccc gccccgccag caccgccgcc agcgtgctct
    10081 tgccgctgcc gttcggcccc ataatcgcgt gcacttctcc cgggtaaatg cgcaggttaa
    10141 tgcctttcag gatgggtttg ccctcgaccc cggcggtcag gtcctgtatc tccagtatcg
    10201 cttgcttcat aggtttggtt cccgttccag cggttctttc gggctcagcc cacgctcccc
    10261 tccaggctca cgcccagcag tttttgcgcc tcgacggcaa attccatcgg cagctcgcgc
    10321 aacacttcct tgcagaagcc gttgacgatc aaggtcacgg cgtcctgcgt cgccagcccg
    10381 cgctggttgc agtaaaaaat ctggtcctcg ccaatcttcg aggttgaggc ctcgtgctcc
    10441 acccgcgcgg tggcattccg gacctcgagg taggggaacg tatgcgcgcc gcaccgatca
    10501 cccagcagca gcgaatcgca ctgcgagaaa ttccgcgcgt gcgccgcgct cttctgcact
    10561 ttcaccaggc cccggtagca gttctgcccg cggccggccg agatgccctt ggacacgatc
    10621 gtgctgcgcg tattgcgccc cacatgcacc atcttggtgc cggtgtcggc ctgctggtaa
    10681 ttgttcgtca ccgccaccga gtagaattcg cccgtcgagt ggtcgcccag caggatgcag
    10741 ctcgggtatt tccatgtgat ggccgagccg gtttccacct gcgtccagct gatcttcgag
    10801 tgcttgcctt tgcacaggcc gcgcttggtc acaaagttgt aaatgccgcc ccggccttcc
    10861 tcgtccccgg gataccagtt ttgcacggtg gaatacttga tctgcgcctg gtccagcgcg
    10921 atcaattcca ccaccgccgc gtgcaactgg tgctcgtccc gcatgggcgc cgtgcagccc
    10981 tccaggtagc tcaccgacgc cccctcctcg gccacgatca gcgtccgctc gaactgcccc
    11041 gtgttggccg cgttgatgcg gaaatacgtg gaaagctcca tcgggcagcg cacccctttg
    11101 gggatgaagc agaacgagcc atcgctgaac accgccgagt tgagcgccgc gaaaaagttg
    11161 tccgtatagg gcaccaccgt cccgaggtat ttgcgcacca gcgcggggtg ctcctgcacc
    11221 gcctccgaga aggagcagaa gatgatcccc ttctccgcca gttgcttttt gaacgtcgtg
    11281 cccaccgaga cgctgtcaaa caccgcgtcc accgccaccc ccgccagccg cgcccgttcc
    11341 ttcagcggga tgcccagctt ttcgtaggtc tccagcagct tcgggtccac ctcctccagg
    11401 ctccgcggcc cgtccccctt ctgcttcggc gccgagtaat acaccgagtc ctggtaatca
    11461 atcggcggat aatgcacatg cgcccagttc ggctcccgca tcgtcagcca atgccggtac
    11521 gcccgcagcc gccactccag cagaaactcc ggctcccgct ttttggccga aatgaaccgg
    11581 acaatatcct cgttcagccc cggcggcgcc gactcggtct ccacgtcggt cacaaagccg
    11641 tacttgtatt ccttccgcac caatcccgcg atggtttcgg ttgctgtgct catgttaatt
    11701 ccatccactg cggcggcgcg cgttcgggcg cgccgctttc ctgtcgctca tatccatgct
    11761 cttaaccgct cctccgtttc ccccgccggc cgggccgggc cggcgcgcac gccgcgcagc
    11821 gcccatgaat ggccagctca taccgctcca cccggcaccc gggcggcagc gcaatccccg
    11881 gcgccgccgg caaatccacg tcaaacacgc gctcgcagac cgtgcaataa aaatgcccat
    11941 gcggccgcat attcgggcaa aaccgcgccg cgccgcgctc cagcgccacc agccgcgcca
    12001 cctcgcattt caccagcgcg tccaggcaat tataaaccgt cgccatcgag atctccggca
    12061 tcttccgctt tgcccggatg aatacccgct cggccgtcgg gtgatcccgt tgctgtaaaa
    12121 gcacagcata cacctgctgc cgctgcggcg taaaacgaaa accgcgccgc gccagccgct
    12181 catccaccct caactcgtcc cgcaggtcca tggctgcttc caccacgggg ccattctacc
    12241 ccgccgcccg cgccttgtca atacttggaa caattctaac ccagacattt cacccctgca
    12301 aggctggagc gcgggcatcc tgcccgctta cagcctccca acgggctaag cgggcaagat
    12361 gcccgcgctc cggcctgcgg gtatttcgtg cggggcgtga aatatcccgg ctaagtccgg
    12421 cggaggcccc tacggcggcg gcatgtggta tggctggccg cgctggtaca gggccatccg
    12481 ttcctcggcc tgccgggcga tgtccgcctg cccggcggcc tgcgccagct cgcgggcccg
    12541 ggcggcggcc ttcagggcct cgtcgaaacg cccggcttcg gcgtacgcgg cgtccagcgt
    12601 gccccagatg cgcgcctccc ggccgccgtt cagttcccgc gcccgctccg ccagccgcac
    12661 ggcttcggcc ccgtcgcgca ggtcggggcg ggcggtggtg gccaggaacc acgcgaggtc
    12721 gttcaggtac agcaccgtat tcgggccgag ccgcgcggcg tcgcgatagt gggccagcgc
    12781 ctcggcggcc tgcccctggc tgtccagcac cagggccagg taatgatgca ggtgcgcggc
    12841 gggccgcagc tccagcgcgc ggcggaactg cgccgccgcc gcggccggct ggttcgcgcc
    12901 cagcaacgcc agcgccaggt tttgccgcgc ctcgacatcc tccgggtgca gccggcacac
    12961 ctcggcatac tgggccgccg cgccggcgaa ctcgccccgg ttgttcagcg caaacgccag
    13021 gttgaaacgc gcatcggcat tctccggcgc ggcccgcacg gccgcctgaa gataccgcag
    13081 cgcctcttcc tcccggtgct gccccgacag caagatcccc agggccatga gcgacggcgc
    13141 gtggttcggc cggagccgca gcgccttcgt gtaagcctgt tccgcttccg ccgcgcggcc
    13201 ccgttgttcc tccatccggc ccaggttatg ccaggtttcg acggtttccc gcttctgcaa
    13261 cgaccgccgg agcaattcct cggcctcgtc cgtccgcccc tctttcaggc ggcacaacgc
    13321 cagattcccc agcccctccg ggtaattgct cttaatccgc accgccttcg tgaaatgccg
    13381 cagcgccgcc gcctgctggt ccgcgtccag cagcaccacc cccaggttgt tatgcgccac
    13441 gaagttctcc cgcgtcacgg ccagcgcgtg ctcgaacaag ctcgtgctgt cgcgccaaaa
    13501 ccccgcttgc cgccacgtca gcgcgcccgc cgccgccagc aacgccaccg cgcccgccgc
    13561 cgccagcgcc accgcggccc gcgcccgccg gcggaccagt tccgccccgc tccacacccc
    13621 cgccacgcac aacccgatca acgggaggta agtatagcgg tcggccatgg cctgctgccc
    13681 cacctgcacc agcccgatca ccgggatcaa catcacccca taccaaaacc agccaaacgc
    13741 aagccacggc gcccgccgcg ccgcgcggac aaccaggacc gacaccccca ccagccccag
    13801 cgccgccagc gccacccaca acggcggcca ttccgccggc gcgggataaa acaccgccag
    13861 cttcgcgggc cacaccagct tgcccgcata ccgcacataa gaaatcagcg cgttggtaat
    13921 ccgaaactcc agcggaatcg tttccagcga atgaaccgcc ccggccttcc gctgaacgag
    13981 gaacgtcacc acgcacgaag cggcggacag gacggcgaaa gggaccttct cccacaaaag
    14041 acgggtcagg acgggcgccg ccagcgaaac gggggagccg ccgcccggcg cggacgccgc
    14101 cccgccgcct tcgcgctccc gccaccgctc cagcggccaa taatccagca gcaacagcac
    14161 gaggggcacc gtcaccagca tgggcttgct catcagcccc agggcaaaga aacccagggc
    14221 gaaaagatag caaccca
//
LOCUS       DAOPQG010000092        13761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5212_length_13761_cov_14.753101, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000092 DAOPQG010000000
VERSION     DAOPQG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13761
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5212_length_13761_cov_14.753101"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /gene="ssrA"
                     /locus_tag="PKI07_10415"
     tmRNA           <1..282
                     /gene="ssrA"
                     /locus_tag="PKI07_10415"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(335..1738)
                     /locus_tag="PKI07_10420"
     CDS             complement(335..1738)
                     /locus_tag="PKI07_10420"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOC51504.1"
                     /translation="MSAKRRSTSPAVSLPRAHGWLALDRRAFLRQSLACVGGLATGLP
                     AIAAGLDRGKAVPLGVARGIHPGRVVWAHDPEVTTWKGPDDGHWWEGNRVKLERVEAM
                     LARAVCELTGETTVPRAWSRLFRHLNQARGKGDVGYRAGEQIVIKPNWVGMIFHEGNV
                     NLDTYAFIRRQDYMNTAPQMILALLDQLVRAGVAPRHITICDTLACLVHDFYDRLHGD
                     FPAVRYEDYAGKFERAKVRTSTQPLYWSSRPEGKAQDYLPASFADAEYLINFANLKAH
                     TGGGVTLCAKNHYGSLVRWPAQKGYYDIHPNCFSRNPRIYRPLVDLTGHSHLGGKTVL
                     YLVDGLFSGVHPRNPVPQRMRTPPFNGQWSCSLLASQDPLAIDSVGFDFLAAEWPDLA
                     GKDGVDDYLHEAALAPDPPSGTFYDPNHDTPTRRLPSLGVHEHWNNPRERKYSRNLGT
                     GDGIELVSVKLGARPGK"
     gene            complement(1824..2663)
                     /locus_tag="PKI07_10425"
     CDS             complement(1824..2663)
                     /locus_tag="PKI07_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51505.1"
                     /translation="MMSLTNGAAERRPKTPGKRMRLAGLAALLAAGLATGCAHSARRS
                     ALADGVASIQVPPVPVFLNGPMALLFTNVNGFRARVVLESGSPARTEAAGELMGQGGH
                     LLFMPAAGGAAARRAPAADSAFIWNVPAQSGFLLNGPMQAYAPIPAGSRPGSLATRAA
                     ADSAAAERIDGHLCECVHAVVAADDGTTSPFRLWRAPDLNGLPLRIIAPAAAAPLTLT
                     LSNARLEPLPRELFLPPKGFTKYDSAEALARELMIRRHNLKRPTPYTSDEDIPRGNLL
                     LPP"
     gene            complement(2775..4349)
                     /locus_tag="PKI07_10430"
     CDS             complement(2775..4349)
                     /locus_tag="PKI07_10430"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOC51506.1"
                     /translation="MRIGLFAWLILLAALTAPTRGAESYDLVIRHGRVVDGSGNPAFF
                     ADVAVRQGRIAALGRIERAGRIEINAAGLIVAPGFIDVHTHADEVATRPLAENFVRMG
                     VTTVVAGNCGSSTLDVARFFRGIERTNAAVNVATLIGHNSVRRKAMGGSFARPPTPAE
                     LAQMKALVGQAMKDGAAGLSTGLIYQPGVHAQTDEIVALAKVIAPYGGIYASHMRHEG
                     AQIYQALDEVFQVARAAGIRAEVSHLKLSGPNAWGQAGQVLARLEQARAEGLDITQDQ
                     YVYTASSTGLSQLLPAWALEGGRDEFRQRVADPALKARLIADLKRRLRDQKRDDFSYA
                     VIASCAHDRALEGLNLAQAAQAKRGSAALEDQIETLLEIQARGGASGVFHNMNEDDLQ
                     VFLRHPNTMVACDSGLRKFGEGMPHPRGYGNNARVLARYVRELKVLRLEDAIRKMTSL
                     PANTFRLPDRGLLRPGAWADLVVFDADKVQDRATFPAPHQYPDGIPHVIVNGIPVIQN
                     GQHTGAKAGKALRHSR"
     gene            4633..6096
                     /locus_tag="PKI07_10435"
     CDS             4633..6096
                     /locus_tag="PKI07_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOC51507.1"
                     /translation="MPGRGGREPRRKLVYFSLVLLAGVLAAAADSRPNILFCLADDWS
                     WPHAGVYGDRVVHTPNFDRIARAGVRFNYCFSTVSSCSPSRAAMLTGQYPHRLEEGSC
                     LWGFLPRRYPVYPKLLEKAGYVIGSTRKGWGPGDFRAGGYTRNPAGQAFPSFDAFLQT
                     VPAGTPFCFWFGSHDPHRPYEAGSGAGIGLKTNKVVIPPYWPDNAVSRQDVLDYYWKV
                     QRFDRDVGEVLAALDKAGRRDNTLVIISGDNGWPFPRCKANVYDGGSRQPLAVRWPAR
                     VKAGRVCEEFISLMDLAPTCLEAAGLPPLPAMTGRSFLGLLTGAEKPGTRNTVFLERE
                     RHANARAGDAGYPVRAIRTREFLYVRNLRPDRWPAGDPQAHKDPLSAFGDCDDGPTKS
                     FILDHRDEPAVRQFYQLCFARRPAEELYDLRRDPHQINNVAGQPAYAAAQKQLRARLD
                     QWQKDTADPRLANDDDHWDRYPYFGPRARMQQRPAQP"
     gene            6359..10291
                     /locus_tag="PKI07_10440"
     CDS             6359..10291
                     /locus_tag="PKI07_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOC51508.1"
                     /translation="MKTPSTSIPVRKRRHSASVLALWLAGATVFAAPVSAANFGDFPA
                     SPPRLPSGASGADGGPRAAASSAEAWTQLHRDAENTGRADFIVPTNRMGTNFFAVLRW
                     QTPAPGSPNEGNLSCSAMVFFDGVGPDGADLVVSGYHWPKGVQSMDRHTGQVFWSGNP
                     AGGESIGVNTPAFSPDGATIYVINDDTPHPLMAFASAEGPSVYWHNEADADPERLGIA
                     SPKVAPDGRIFAHAWNDRPYAGTDSGTDILVTWEAESELCECLSRPAIWTTAARTNVL
                     AAGRCAELKAFDGASGDEWWSVTGGLETDADPTVDPASGRVYLPIGAASIRIVGVDAD
                     GEPLWSQVAMPVFDWQDGVNHPQRAQSAGCLAQDGLTFYFQTVSQEGDGRLYAINTAD
                     GSVKWSYATGSKGWDSEASSPIVTPNGVLVVGNNEGGTYFALRDDGANATLLDTLTVA
                     ANGTARSTATLSSDGLLYLPARLVWTQSNGDGQTPSQQAANLYNAFDLNAAPEIHLPP
                     PAGQAARPLNAAIELQWLPVPDPMGHFSHYAIYRAPEPFTSLAGLTPVATLSNRLATT
                     YLDSPLVNGASYYYFVATVTLSGGEPGTVTAIGGFRPYDETDLQVVGISRTPRFPRYA
                     AEYTDYEITEPSGYGPYRFSAATGLGHGQTTNTPRWPAINAPVTYTATVRNRGSNPWT
                     NAIAGVWQWDGTNAASNSVAGPLAPGGRTTFTFARPWDGAAHDVRFLLTTSDARAGNN
                     AVTINTKSVAFLSYIDASYYESFRFQTTNWANAVTDDLIDWIQYHMARFNQMFASANC
                     AKRVHYDVLELLADTAPDPAVDTLPFAVFPFRFYAHEAPYSYRNSGWYNDADDIDYGY
                     LHEMGHQLGLIDIYQLDLSGEMNQVSGLGYTGPDDLMRACSPFFSPHSALAMNHWLDQ
                     AHGYFGQYLYGIPAEVKMRFLTRTGQPLHGAAVKVYQLEERPDEGRLITPQVKAQGLT
                     DSNGVLVLPNVPIDPAKVPPLPTGDVLRANPFGYVAVTGANGVLHFRLEYEGVVDYAW
                     LDITEANVAYYQGETNQATFTRQVTLGGPIQRFPPRELTETNAADWVAWAEGGASGGS
                     TVTNDASRRQVGQVSLKFVTDGGFDTWVRYPGTFNALWDLTEVSTLNIRFYVENPNLG
                     FQNGSPWIRLKDGDGNYVQYQYYQDGWPYELLNDARDAWLACQIPLAASPTEENGWRR
                     STFGAPDLSRITALEIHADTWGSGFTLWIDGVSFEPPRRPKLSVAPEAGGIAVSWPAT
                     HPEPVLEAANHVLGPYAPVAVMLTEAGGIATAQLPATGGQRYFRLRMP"
     gene            complement(10377..13124)
                     /gene="uvrA"
                     /locus_tag="PKI07_10445"
     CDS             complement(10377..13124)
                     /gene="uvrA"
                     /locus_tag="PKI07_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOC51509.1"
                     /translation="MFDDRGQMGGPESAIVIHGAREHNLKNVSLAVPRGRLVVITGVS
                     GSGKSTLAFDLLFAEGQRRFLDSMNVYARQFVEQLARPDVDLIAGIPPTVSIEQRISR
                     GGGKSTVATVTEVHHFIRLLFARLGTPFCPECRVPVEAQTRDELGRRLQRELRQRGDL
                     RLLAPVVKNRKGFHTDVGEWAARHGYAEVRADGRFYSTRQRLRLDRFREHDVEIVVGV
                     LKANGRPPGGRTPPRIRAASANGALGDPHPPRLEGQRLIDETLKLGQGTLFALDSHQR
                     LTIHSTERACPQCGRSFAVLDPKNFSYNSPQGWCPRCRGFGELFYLPEADRGARADAI
                     EESWYGWQEGQRETCPECGGSRLNPLARAVRLGCAECGAREAGWETAPTMDTFGRMSV
                     AEADHFFRRLRFAARAALIARDILPEIRERLKFLREVGLDYLQLGRGVPTLSGGEAQR
                     IRLAAQLGSNLSGVLYILDEPTIGLHARDNEQLLAALEQLKARGNSVLVVEHDEATMR
                     RADYIIDLGPGPGRHGGEVVAAGTLAELRRHPESITGQCLRTKRSFPARGQRRAVGKA
                     RRPAPEPGWLTLRGARKNNLKNLTVSFPLGRLVVVTGVSGSGKSTLVRECLLPALQSR
                     RASRAARRTSARPRLSGAESLHAVYEVDQSPIGRTPRSIPATYVGFFDDIRRLFAQVP
                     EARLRGYSPSRFSFNSAQGRCPECEGAGTIKLEMNFLPPAFVRCEVCGGTRFNRETLD
                     IEYHGRNIAAVLEMSVEEAREFFGNLPRLRRPLQALCDTGLGYLKLGQTSPTLSGGEA
                     QRVKLVSHLLSGLKTPEALERRPRRNLFVLEEPTIGLHMADVRRLVDVLQRLVEAGHS
                     VIVIEHNLDLIAEADWVIDLGPEGGDGGGRVVAAGTPEQVARCRLSHTGRFLRGILAP
                     A"
     gene            complement(13153..13229)
                     /locus_tag="PKI07_10450"
     tRNA            complement(13153..13229)
                     /locus_tag="PKI07_10450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13193..13195),aa:Arg,seq:tct)
BASE COUNT         2193 a         4629 c         4697 g         2242 t
ORIGIN      
        1 catcggaccg aacaataatt gctaacgaag aattagctct cgcggcctaa gcgccacgac
       61 gttcaccggc ggacacctgc tacgccggat gagcgccaca gcaggagtag gctcgacgcg
      121 gcgtccgcgc gcggcgggtt gaagttctta tcctgcgggc taggctgggc gactcggagt
      181 caggccgcca tcgcccggca gaaaactctc tcctgactaa gcatgtagtc gcggctggca
      241 atctggctaa ggacccgggt tcaactcccg gcgcctccac cactttcccc ccggccccgc
      301 gcccgcgggg atgcgcccgg ttggcgggga gccgctattt tccggggcgg gcgcccagct
      361 tcacgctgac gagttcgatg ccgtcgccgg tcccgaggtt gcgcgagtat tttcgttccc
      421 gcggattgtt ccagtgttcg tggacgccca ggctgggcag ccgccgggtg ggggtgtcgt
      481 ggttcgggtc gtagaaggtt cccgacggcg ggtcgggggc cagcgccgct tcatggaggt
      541 aatcgtccac gccgtctttg cccgccaggt cgggccactc ggcggccagg aagtcgaacc
      601 cgaccgagtc aatggccagc gggtcttgcg aggccaggag gctgcaggac cattggccgt
      661 tgaacggggg cgtgcgcatc cgctgcggaa ccgggttgcg gggatgcacg ccggagaaca
      721 ggccgtccac gaggtagagg acggtcttgc cgcccaggtg ggagtggccg gtcaggtcca
      781 ccagcgggcg gtagatgcgc gggttgcggg agaagcagtt ggggtggatg tcgtagtagc
      841 ccttttgcgc cggccagcgg acgagggagc cataatggtt tttggcgcag agcgtgacgc
      901 caccgccggt gtgggccttg aggttggcga agttgatgag atattcggcg tcggcgaagc
      961 tggcgggcag gtaatcctgg gccttgccct ccggacggct gctccaatag agcggctgcg
     1021 tggaagtccg gactttggcc cgctcgaatt tgcccgcgta atcctcgtag cgcacggccg
     1081 ggaagtcccc gtggaggcgg tcgtagaagt cgtggaccag gcaggcgagc gtgtcgcaga
     1141 tggtgatgtg ccgcggggcc accccggcgc gcaccaactg gtcgagcagc gcgaggatca
     1201 tttgcggcgc ggtgttcatg tagtcttgcc ggcggatgaa ggcgtacgta tcgaggttga
     1261 cgttgccttc gtggaagatc attcccaccc agttgggctt gatgacgatt tgctcgcccg
     1321 cccgataccc gacatccccc ttgccccgcg cctggttcag gtggcggaac agccgggacc
     1381 aggcccgggg aaccgtggtt tcgccggtca attcgcagac ggcccgcgcc agcatggcct
     1441 ccacgcgctc cagcttcacc cggttgccct cccaccagtg gccgtcgtcg gggcctttcc
     1501 aggtggtcac ctcgggatcg tgcgcccaga ccacgcgccc cgggtggatg ccccgggcca
     1561 cccccagggg gacggccttg ccccggtcga ggcccgcggc gatggcgggc aggcccgtgg
     1621 ccaacccgcc cacgcatgcc agcgattgac ggagaaaggc gcggcggtcc agggcgagcc
     1681 agccgtgggc gcgggggagg gaaaccgccg gagaagttga acgccgcttt gcactcatgg
     1741 ggcaacacta cgcgggcgga cgcagatttt caagggggga gccggcgccg cgcccgggcc
     1801 gcgcggcgtg gtgacagcgg gtcttaaggc ggcaagagca ggttgccgcg ggggatgtcc
     1861 tcatcggacg tgtagggggt cgggcgttta aggttgtgac ggcggatcat cagttcacgc
     1921 gccagggcct cggcgctgtc gtatttggtg aagcctttgg ggggcaggaa aagttcgcgg
     1981 ggcaggggtt ccaggcgggc attggacagg gtgagcgtca ggggcgcggc ggccgcgggg
     2041 gcgatgatgc ggagcggcag gccgttcaag tccggcgccc gccacagccg gaacggggag
     2101 gtcgtgccat cgtcggcggc cacgacagca tggacgcatt cgcagagatg gccgtctatg
     2161 cgctcggcgg cggcggagtc ggcggcggcg cgggttgcca ggctgccggg ccgggagccg
     2221 gcggggattg gcgcatacgc ctgcatgggg ccgttgagga ggaagccgga ttgcgccggt
     2281 acgttccaga tgaaggcgct gtcggccgcc ggagcacgcc gggcggccgc cccgccggcg
     2341 gccggcatga agagcaggtg gccgccttgt cccatcaatt cgccggcggc ttccgtccgg
     2401 gcgggggagc cgctttccag caccacacgg gcgcggaacc cgttgacgtt ggtgaacagc
     2461 agcgccatcg ggccattcag gaagacggga accggcggga cctggatgga ggccaccccg
     2521 tccgccagcg cgctccgccg cgccgagtgg gcgcaaccgg tggccagtcc cgcggccaac
     2581 agggcggcca gcccggcgag ccgcattcgt ttcccgggcg ttttcggccg tcgttccgcc
     2641 gcgccatttg tcaggctcat cataccgcgc agcataccgg atcggcggcg ggcgggcaaa
     2701 gccgaactgc ggcgccggga agcgaagttc ccccgggagg ctgaggcaga gggcgggccg
     2761 ggcggggggc ggcgtcagcg cgagtggcgc aaagccttgc cggctttggc gccggtgtgc
     2821 tggccgttct ggatgacggg gatgccgttg acgatgacat gcgggatgcc gtcggggtat
     2881 tggtgcgggg ccgggaaggt ggctcggtcc tgcactttgt cggcgtcgaa cacgacgaga
     2941 tcggcccagg cgccgggccg gagcaggccg cggtccggga ggcggaaggt gttggcgggc
     3001 aggctggtca tcttgcggat ggcatcttcg aggcgcagca ctttgagttc gcggacgtag
     3061 cgggccagca cgcgggcgtt gttgccgtag ccgcgggggt ggggcatgcc ttcgccgaat
     3121 ttccgcaggc cgctgtcgca ggcgaccatg gtgttgggat gccgcaggaa aacctggagg
     3181 tcgtcttcgt tcatgttgtg gaagacgccg ctggcgccgc cgcgggcttg aatctccagc
     3241 agcgtttcga tctggtcctc gagcgcggcg gagccgcgtt tggcctgggc ggcctgggcg
     3301 aggttgagcc cttcgagcgc gcggtcgtgc gcgcaggagg cgatgacggc gtaggagaag
     3361 tcgtcccgct tttggtcgcg caggcggcgt ttcaggtcgg cgatcaggcg ggctttgagg
     3421 gccgggtcgg ccacgcgctg gcggaactcg tcccggcccc cctcgagcgc ccaggccggc
     3481 agcaactggc tcaggccggt gctggaggcg gtatagacgt attgatcctg cgtgatatcc
     3541 agcccctcgg cgcgcgcctg ttcgaggcgg gccagcacct ggccggcctg gccccaggcg
     3601 ttgggcccgg agagcttgag gtgcgacacc tcggcgcgga tgcccgccgc gcgcgcgacc
     3661 tggaagactt cgtccagggc ctggtagatt tgggcgcctt cgtgccgcat gtggctggcg
     3721 tagatgccgc cgtagggcgc aatgaccttg gccagcgcca cgatttcatc ggtctgcgcg
     3781 tgcacgccgg gctggtagat caggccggtg gaaagccccg ccgcgccgtc tttcatggct
     3841 tgcccgacca gcgccttcat ttgggccagc tccgccgggg tgggcgggcg cgcaaaagag
     3901 ccgcccatcg ccttgcggcg gacgctattg tggccgatga gcgtggcgac gttgacggcg
     3961 gcgttggtgc gctcgatccc gcgaaagaac cgggcgacgt ccagcgtgga gctgccgcag
     4021 ttgcccgcga ccacggtcgt cacccccatg cgcacgaagt tctccgcgag cggccgggtg
     4081 gcgacttcat cggcgtgcgt atggacgtca atgaatccgg gggcgacgat tagccccgcc
     4141 gcgttgattt cgatgcggcc cgcgcgctcg atgcggccca gggcggcgat gcgcccctgg
     4201 cgcaccgcca cgtcggcgaa gaaggccggg ttgcccgagc cgtccaccac gcggccatgc
     4261 cggatgacca ggtcgtagga ttccgctccc cgcgtgggcg ccgtcagcgc cgccagcaga
     4321 atcaaccacg caaacaaacc gattctcatc cgccgggtta tatccgaaaa tcgggctggc
     4381 ggccacatca acctgcgacc cgcggcgcca gtttcccggc gccggctccg ggcgcgcgcg
     4441 cgcggggaaa gccggttctt attcgccgca aataaggcgt tgaagcgcca gccggctttt
     4501 gataaagtcg gcgcatgctc ggtgagcgca aaagcctttt tccccgcccg cgcgtggcgt
     4561 tctgtgcggg cgggagcgcg gaaagcaagt tctcatcggg ttgcgccggc cgggatgaat
     4621 agccataaga ttttgccggg acggggaggg cgggagccgc gccggaagct ggtttatttc
     4681 agcctggtgc tgctggccgg cgtgctggcg gcggcggcgg actcgcgccc gaacatactg
     4741 ttttgcctgg cggatgattg gagctggccg catgcgggcg tctatggcga ccgggtggtg
     4801 catacgccga attttgaccg cattgcccgc gcgggggtgc gcttcaacta ctgcttcagc
     4861 acggtgtcgt cgtgctcgcc gtcgcgagcg gcgatgctga ccggccaata cccgcatcgg
     4921 ctggaggagg ggagctgcct gtgggggttt ctgcctcggc ggtacccggt gtatcccaag
     4981 ctgctggaaa aagccggcta tgtcattggc agcacgcgca aaggctgggg gccgggcgat
     5041 ttccgggccg gtggctacac gcgcaacccg gcgggccagg cgtttccgag ctttgacgcc
     5101 tttctgcaaa ccgtgccggc gggaacgccg ttctgcttct ggttcggcag ccatgatccg
     5161 catcggccct acgaagccgg ttcgggcgcc ggcattggcc tcaaaacgaa caaggtcgtc
     5221 atccctcctt actggccgga taatgccgtt tcgcgccagg atgtgctgga ctactactgg
     5281 aaagtgcagc gcttcgaccg cgacgtgggc gaggtgctgg cggcgctgga caaggcgggc
     5341 cggcgcgaca atacgctggt catcatcagc ggcgacaatg gctggccgtt cccgcgctgc
     5401 aaggccaatg tctatgatgg cggcagccgc cagccgctgg ccgtccgctg gccggcccgg
     5461 gtcaaagccg gccgggtttg cgaggagttc atcagcctga tggatctcgc gccgacctgc
     5521 ctggaagcgg ccgggcttcc gccgctgccg gcgatgaccg gccgcagctt tctggggctg
     5581 ctgaccggcg cggagaaacc cgggacgcgc aacaccgtct ttctcgagcg cgagcggcac
     5641 gccaacgcgc gcgctggcga cgcgggttac ccggtgcgcg ccattcgcac gcgcgagttt
     5701 ctttacgtgc gcaatcttcg gcccgaccgc tggccggccg gggatccgca ggcgcacaag
     5761 gatcccctta gcgccttcgg cgactgcgac gacggcccga ccaagagctt catcctggat
     5821 caccgcgatg agccggcggt gcggcagttc taccagcttt gcttcgcccg gcgcccggcg
     5881 gaggagctct acgatttgcg ccgggacccg caccagatca acaacgtggc cggccagccc
     5941 gcctatgccg ccgcgcaaaa acagctccgc gcccgcctgg accagtggca aaaagacacc
     6001 gccgacccgc gcctcgccaa cgacgacgat cactgggacc gctatcccta ctttggcccg
     6061 cgcgcccgga tgcaacagcg tcccgcccag ccttagcccg cgcgggttcc gcgtggattt
     6121 acgggcttgc ggacaggggg aaaagctgga gacccgggcg cccggatgcg atgccctggc
     6181 gcgaaaaaac ccgttgaccc gcgggccgct tttcggtaat gtcatggcat gtccagtgag
     6241 cttgaaacca catccctgtt ttcccccctg ccatactccg tgccggcggg ccgtggcttc
     6301 ggctctttcc cgtcccggga aactcaccaa aaccttccga cctgataaag cccaaactat
     6361 gaagactcca tcaacttcca ttcccgttcg caaacggcgg cattccgcca gcgtgctggc
     6421 cctctggctg gccggtgcga cggtcttcgc cgcgccggtt tctgccgcaa actttggcga
     6481 tttccctgcc agcccccccc gtctcccttc gggagcatcc ggggcggacg gcgggccgcg
     6541 cgcggcggcc tcgtcggcgg aggcctggac gcaactccac cgcgatgccg agaacaccgg
     6601 ccgggcggat ttcattgttc caaccaaccg gatggggacc aatttcttcg cggtgctgcg
     6661 gtggcagacg ccggctccgg gctcgcccaa tgaaggcaac ctgagctgca gcgcaatggt
     6721 gttttttgac ggtgtgggcc ccgacggggc agacctggtg gtgagcggct atcattggcc
     6781 caaaggggtg cagagcatgg accggcacac gggccaggtt ttctggagcg gcaatcccgc
     6841 tggcggcgaa tccatcggcg tcaacacgcc cgccttttct ccggatggcg cgacgattta
     6901 tgtcatcaac gacgatacgc cgcatccgtt gatggcattc gcgtcggccg aggggccttc
     6961 ggtttactgg cataacgagg cggatgccga tccggagcgg ttgggcatcg cttcgccgaa
     7021 ggtggcgccg gacggccgca tcttcgcgca cgcctggaat gaccggccgt acgcggggac
     7081 cgattcgggc accgacatct tagtcacctg ggaggcggaa tcggagttgt gcgagtgcct
     7141 cagccggccg gcgatctgga ccaccgccgc gcgcacgaac gtgctcgccg cgggccgctg
     7201 cgccgagttg aaagcgtttg acggcgccag cggcgacgag tggtggagcg tcacgggcgg
     7261 cctggagact gacgccgacc cgacggtgga tccggcgtcg ggccgggtct atctgcccat
     7321 cggcgcggcg agcatccgaa tcgtcggcgt ggacgccgac ggggagccgt tgtggagcca
     7381 ggtggcgatg ccggtgttcg attggcagga cggggtgaat cacccgcagc gcgctcaaag
     7441 cgccggctgc ctggcgcagg atgggctgac gttctatttt caaacggtca gccaggaagg
     7501 cgacggccgg ttgtacgcca tcaacaccgc cgatggctcg gtgaaatggt cctacgccac
     7561 cggcagcaag ggttgggaca gcgaagcgtc gtcgcccatt gtgacgccca acggggttct
     7621 ggtcgtgggc aataacgagg gcggaactta cttcgccctg cgggacgacg gggcgaacgc
     7681 caccttgctc gacacgctga cggtggcggc aaatggcacc gcccggagca cggccaccct
     7741 ctcgtccgac ggcctgctgt acctgcccgc gcggctcgtc tggacgcaaa gcaacggcga
     7801 tggccagacg ccctcgcagc aggcggcgaa tctctacaat gccttcgatc tgaacgcggc
     7861 gccggaaatc cacctgccgc cgcccgccgg ccaggcggca cggccgctga acgccgccat
     7921 cgagctgcaa tggctgccgg tgcccgaccc gatggggcat ttctcccact acgcgattta
     7981 tcgcgccccg gagccgttca cgtcgctcgc gggcctgacg cccgtcgcaa ccctctcgaa
     8041 tcggcttgcg acgacttacc tggacagccc gctggtgaat ggagcgagct attactactt
     8101 tgtcgcaacc gtcacgctct ccggcggcga gccgggcacg gtcacggcca tcggcggctt
     8161 ccggccgtat gatgaaaccg acctccaggt cgtcggcatc tcccgcacgc cgcgctttcc
     8221 gcgctacgcc gcggaataca ccgattacga aatcaccgag ccctccggct acggccccta
     8281 ccgcttcagc gcggcgacgg gcctgggcca tggccagacg accaacacgc cgcgctggcc
     8341 ggccatcaac gccccggtca cgtacaccgc cacggtccgc aaccggggga gcaatccgtg
     8401 gaccaatgcc atcgccggcg tctggcaatg ggacggcacc aacgccgctt ccaactccgt
     8461 cgccgggccg ctcgccccgg gcggccggac gacgttcacg ttcgcgcgtc cgtgggacgg
     8521 cgcggcgcat gacgtccgct tcctcctcac cacgtccgac gcgcgggcgg gcaacaatgc
     8581 ggtgaccatc aacaccaagt ccgtcgcctt cctgagttat attgacgcga gctactacga
     8641 aagcttccgg ttccagacga ccaactgggc gaacgcggtc acggatgact tgattgactg
     8701 gattcagtat cacatggcgc ggttcaacca aatgttcgcc agcgccaact gcgccaagcg
     8761 cgtgcattac gacgtgctgg agctgctggc ggacaccgcg cccgatccgg cggttgacac
     8821 cctcccgttt gccgtgttcc ccttccggtt ctacgcccac gaagccccct acagctaccg
     8881 caactccggg tggtataacg acgcggacga cattgattac ggctatcttc acgaaatggg
     8941 gcatcagctc gggctcattg acatttacca gctcgacctc agcggggaga tgaaccaggt
     9001 ctccggcctc ggctacaccg gccccgacga tcttatgcgc gcgtgcagcc ccttcttctc
     9061 gccgcacagc gcgctggcga tgaaccactg gctcgaccag gcccacggct atttcggcca
     9121 gtatctctat ggcatcccgg cggaagtgaa aatgcgcttt ctcacgcgca ccggccagcc
     9181 gctccacggc gcggcggtga aggtgtatca gctcgaagag cgcccggatg agggccggct
     9241 catcaccccg caggtcaagg cccaggggct gaccgactcc aacggcgtct tggtcctgcc
     9301 gaacgtgccg attgaccccg ccaaagtccc gccgttgccg acgggcgacg tgctgcgcgc
     9361 caacccgttt ggctacgtcg cggtgacggg agccaatggc gtgctgcact tccgcctcga
     9421 atacgagggc gtggtggatt acgcgtggct cgacatcacc gaggccaacg tcgcctacta
     9481 ccagggcgag accaaccagg ccaccttcac gcgccaggtc acgctcggcg gccccatcca
     9541 gcgctttccg ccgcgcgaac tcaccgagac caacgccgcc gactgggttg cctgggccga
     9601 gggaggcgcc agcggcggca gcaccgtcac gaacgatgcc tcgcgccggc aggtcggcca
     9661 ggtctcgctt aagtttgtca ccgacggagg attcgatact tgggtccgct accccggcac
     9721 cttcaacgcg ttgtgggacc tcaccgaggt cagcaccctg aacatccggt tctatgtcga
     9781 gaatcccaac ctgggctttc aaaatggcag cccgtggatc cggctcaagg atggcgatgg
     9841 caactacgtt cagtatcaat attaccagga cggctggccc tacgagttgc tcaacgacgc
     9901 ccgggacgcc tggctggcct gccagatacc gctggcggct tcgcccacgg aggaaaacgg
     9961 ctggcggcgc tccacctttg gcgccccgga cctgagccgc atcaccgcgc tggaaatcca
    10021 tgccgatacc tggggcagcg gcttcaccct ctggattgac ggcgtgagct tcgagccgcc
    10081 ccggcgcccg aaattgagcg tggcgccgga ggccgggggg attgccgtca gttggcccgc
    10141 cacgcatccg gagcccgtgc tcgaagccgc caaccatgtc ctgggtccct acgcgccggt
    10201 cgccgtgatg ctgacggaag ccggcgggat cgccacggcg caactgcccg ccaccggcgg
    10261 gcaacgttac ttccgtctcc ggatgccata gccggcccgg catcgccgtc ccggctcgcg
    10321 ccgcggggcg ccgccaggtt cgcggtgggc cgctggggat tcggcccgcg ccgcgcttag
    10381 gccggggcga gaatgccgcg caggaaccgc ccggtatgtg agagccggca gcgggccacc
    10441 tgctccggcg tgccggcggc cacgacgcgt ccgccgccgt ccccgccttc ggggccgagg
    10501 tcaatcaccc aatcggcctc ggcaatgaga tcgaggttat gctcgatgac gatgaccgaa
    10561 tggccggcct ccaccaaccg ttgcagcacg tccaccagcc gccgcacatc ggccatgtgc
    10621 aggccgatgg tgggttcctc caggacgaac aggttccgcc ggggccggcg ctccagcgcc
    10681 tcgggcgtct tcagcccgga cagcagatgg ctcaccagtt tgacgcgctg cgcttcgccg
    10741 ccgctgaggg tggggctggt ctggcccagc ttgagatagc ccagccctgt atcgcacaac
    10801 gcctgcagcg gccgccggag gcggggcagg ttgccaaaga attcccgcgc ctcttccacg
    10861 gacatctcca gcaccgcggc gatattccgg ccatggtatt cgatgtcgag cgtttcacgg
    10921 ttgaaccgcg tcccgccgca gacctcgcag cgcacgaacg cggggggcag aaagttcatc
    10981 tccagcttga tcgttcccgc cccttcgcac tcggggcagc ggccctgcgc gctgttgaag
    11041 gaaaaccggc tcggcgaata accgcgcagc cgcgcttccg gcacctgcgc aaataaccgg
    11101 cggatgtcgt caaagaagcc cacatacgtg gcaggaatcg agcgtggcgt gcgcccaatg
    11161 ggcgattggt ccacttcata gacggcatgc agcgattccg ccccggagag ccggggacgc
    11221 gcggacgtgc ggcgcgcggc gcgcgatgcg cggcgggatt gcagcgccgg gagcaggcac
    11281 tcgcgcacca gcgtgctctt gcccgagccg ctcacacccg tcaccaccac cagcctgccc
    11341 agcggaaacg aaaccgtcag gttctttaag ttgttcttcc gggcgccccg cagcgtcagc
    11401 cagccgggtt ccggcgccgg ccgccgcgcc ttgcccaccg cgcgccgctg cccgcgcgcc
    11461 gggaagcttc gcttcgtccg caggcattgg cccgtgatgg attccggatg ccgccgcaat
    11521 tcggccagcg tgccggcggc caccacttcc cccccatgcc ggcccggccc ggggcccagg
    11581 tcaatgatat agtccgcccg ccgcatggtg gcctcgtcat gctccaccac cagcacggag
    11641 ttgccgcggg ccttgagttg ctccagcgcc gccagtaatt gctcgttatc ccgcgcgtgc
    11701 aggccaatgg tgggctcatc caggatgtaa agcacgccgc tcaggttcga ccccagttgc
    11761 gccgccagcc ggatgcgctg cgcctcgccg cccgacaacg tcggcacgcc ccgccccaac
    11821 tgcaggtaat ccagccccac ctcccggaga aacttcagcc gttcccggat ttccggcagg
    11881 atgtcgcgcg caatcaacgc cgcgcgcgcc gcaaacctga ggcgccggaa gaaatggtcc
    11941 gcctccgcca ccgacatccg gccaaacgtg tccatcgttg gcgcggtttc ccacccggcc
    12001 tcccgcgccc cgcactccgc gcaccccagc cgcaccgccc gcgccagcgg gttcagccgc
    12061 gacccgccgc actcggggca ggtctcccgt tgtccctcct gccagccgta ccacgattcc
    12121 tcgatcgcgt ccgcccgcgc gccgcgatcc gcctcgggca ggtaaaacag ctccccgaac
    12181 ccccggcagc gcgggcacca gccctgcggg gaattatagc tgaagttctt gggatccagc
    12241 accgcgaagg aacggccgca ctgcgggcag gcgcgctcgg tcgaatgaat agtcaggcgc
    12301 tgatgcgaat ccagcgcaaa cagggtcccc tggcccagct tgagggtttc gtcaatcagc
    12361 cgctgccctt ccaggcgggg agggtggggg tccccgagag ccccgtttgc cgaagcggcg
    12421 cggattcgcg ggggcgttcg ccctcccggg ggccgcccgt tcgccttcag cacgccgaca
    12481 acaatctcca cgtcatgctc gcgaaagcgg tccaggcgca gccgctggcg ggtggaataa
    12541 aaccgtccgt ccgcgcgaac ctccgcgtag ccgtggcggg cggcccattc tcccacatcg
    12601 gtgtggaacc ccttgcggtt cttcaccacc ggcgccagca accgcaggtc gccgcgctgg
    12661 cgcagctcac gctgcaggcg gcggcccagt tcgtcgcggg tctgggcctc gaccggcacg
    12721 cggcactccg ggcagaacgg ggtgcccaac cgcgcaaaga gcaggcggat gaagtgatga
    12781 acctccgtga ccgtggccac cgtgctcttg cccccgccgc ggctgatgcg ctgctcgatg
    12841 ctcaccgtgg gcgggatgcc cgcaatcaaa tccacgtccg gacgcgccaa ctgctccacg
    12901 aattgccgcg catagacgtt catcgagtcc aggaaccgcc gttgtccctc ggcaaacagc
    12961 aggtcaaacg ccagcgtgct cttgccggag ccgctcaccc cggtgatgac aaccagccgc
    13021 ccgcggggca ccgcgagcga cacattcttc aggttgtgct cccgcgcccc gtgtatcact
    13081 atggcggact ccggcccgcc catctgtcct cgatcatcaa acatcgttat tgcaaccgcc
    13141 tggcccaaaa aatggcacgc ccgttgggac tcgaacccaa gaccctctgc ttagaaggca
    13201 gatgctctat ccaactgagc tacgggcgcg cccggggaac gctgggccgg gaggggtgga
    13261 aggtggatcg tcggggcgcg tgcggcagcg gccttggcct cgaatcatgg cggcgccggt
    13321 cgggagcaag tcatcttgcc ggttttgcga tgtcaccttg ccagctctgc ggttatggtt
    13381 ccgtaacggc acatatccag tgccgcagca ccttaatcgg catggccgcc ggcgtcaagg
    13441 cggcgccacg ctccgcggtc aaatggagca gggccgttga tttttagatc atgagccggg
    13501 gaggctggga ttgcgggtgg gacggcggtt atgcgcttcg gagaggatgc cttgccgtgc
    13561 gcccagggcc cgggcaggtg ggaacagatg gggttggctt acttcgtccg gaaggggatg
    13621 gtcgccgcca ttttgcccga tcgccggggc cagtttgcgc tggacgtgca agggatcggc
    13681 agcgatgagc ttgccctcca agaccagcag cccttcccgc agacaatccg ggcaggcgcg
    13741 caggtgcttt tctcagcgcc g
//
LOCUS       DAOPQG010000093        13734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5220_length_13734_cov_15.903282, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000093 DAOPQG010000000
VERSION     DAOPQG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13734
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5220_length_13734_cov_15.903282"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1506
                     /locus_tag="PKI07_10455"
     CDS             64..1506
                     /locus_tag="PKI07_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOC51510.1"
                     /translation="MSDVLHRLAALLPADRLLTGPAQLAAYESDGLTAYRTRPQAVAI
                     PETTDEVVALVRFCHQEKLPFVARGSGTSLSGGSLPVADGIVIALNRLTRILRLDPAQ
                     RIAVVEPGVVNTQVSVAAAAHRLHYAPDPSSALICTIGGNVAFNSGGAHCLKYGMTAN
                     HILGLKAVLPTGEVVEWGGASREHIGPDWCGLFNGSEGLFGIALEITLQLLPRAECFY
                     TVLAGYRTLEQAGDAVSAVVASGLLPGALEIMDALALEAATAAVHAEYPPGAEAVLIV
                     ELEGTREAVAADRGRLEAIIAGSQPIEMRPARDAEERLAIWKGRKSAFSAVGRLSPDF
                     IVQDGVVPRRRLGEALRHNGELARAAGLRVANVFHAGDGNLHPLILFDGREAGALERA
                     EALAGRILKRCVEMGGSITGEHGVGVEKLEYLPAMYNADELDCMMRLRAAFDPLGIAN
                     PGKKFPRAGAPALSQRGLHPLERAGVISRE"
     gene            1512..2600
                     /locus_tag="PKI07_10460"
     CDS             1512..2600
                     /locus_tag="PKI07_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOC51511.1"
                     /translation="MNEPLTPATPEALAEAVRAHPRVLAIGARTKPRLSRMPAEVTLI
                     STRALRGMVEYEPDEFTFTARAGTPVREIERELAARGQYLPFDPLWLDAGATLGGTVA
                     AGLSGPGRFRFGGVRDFILGIRFVDGMGRLLRMGGKVVKNCAGFDLPKFMVGGLGRYG
                     ALAELSFKVFPLPAARLTLQLPTEGAEAAARVFTEAAGARWECEALDLLPDGQTVCLR
                     LAGPAPAIEAVSREILARWPGQALAPDGAEALWAEVREFRWAHADGVLAKVALTPAAM
                     PALDAAVRSLKGARLHFSAGGNLAFVSLPPAVALTALPERLRALGLAGLTLRGGAPLW
                     PGRHTIPAIAGAVKQALDPDNRFPPTED"
     gene            2600..3868
                     /locus_tag="PKI07_10465"
     CDS             2600..3868
                     /locus_tag="PKI07_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOC51512.1"
                     /translation="MLHSIQPERYAPFGEAMTRAVQACVHCGFCLSVCPTYAELREEA
                     DSPRGRIVLMKLVLEGALAWEAAQPHIDRCLGCLACEPACPSGVPYRELISPFRALAA
                     GKFHRSGADHLRRWLAAKTIPHPARFRLAVRSAGAGRALRPLVPRALESMLDLAPRCV
                     PPAQTWPAVTPARGERRARVALLTGCAQQVLDPDINTATLEVLARNGVEVLIPGRQCC
                     CGGLAWHTGDWPAARDFARRNLAAFPDDVDAIITNAAGCGSTLHEYPLILRGTADEAR
                     AEAFARRVVDVSVFLTRLGLREKPAGTGRPQRIAYHDACHLAQAQDIRSEPRDLLRAI
                     PGVKLLELPDTHLCCGSAGSYNLDQPAIAAALGEKKARAVLASGAEVLASGNIGCLTQ
                     LRAHLARLGAELPIRHTIQVLRDAYQPEPR"
     gene            complement(3929..4768)
                     /locus_tag="PKI07_10470"
     CDS             complement(3929..4768)
                     /locus_tag="PKI07_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOC51513.1"
                     /translation="MRNLPDKFLGAMIAVLPLPGAPRYGGDDARIIAQALSDVAHYHA
                     AGVDALVLENSHDLPYIKPPLPPRAIKVMQQVADAVRRQFPGPIGIQMLEAANETALE
                     IAGTAGLDFLRVEGYVFAHVGGAGLIEGCAGRLLRRRRELGVEQIKIFGDVKKKHCSH
                     ALTGDLDILDEVKQAEFFLVDGVIVTGARTTEPPALAELQRVQEHARVPVLIGSGITP
                     ENLAAYFPRADGFIVGSTFRQGGRFLGDLEPKRLEAFMRVFRRLRAEHEKGSGTARSK
                     PGT"
     gene            complement(4778..6379)
                     /locus_tag="PKI07_10475"
     CDS             complement(4778..6379)
                     /locus_tag="PKI07_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51514.1"
                     /translation="MTPPALPRRRFLQLMATAAASAPAWPRGGGARAQPAAADDPSPL
                     PFETALAGPARGPWRRLFLDAWAVEEQQGLTRVFHAAEKHPANPVLKGDQPWESVPAA
                     ITGPYVYGTAAWDGGKLRLWYQILNRGNHVGYAESADGIHWTKPALGLIEFNGSKANN
                     LCVSAAQPEVTGGECHNPGVLRCPEPAAPHQRYALFGFDPRAGHARVAFSPDGRRWKY
                     VPETEKKPLFSSSDVVNFFYDPGQKRYTATWKTHSRRGRAVGIAWSPDGLAWTKPYDG
                     PLFAADDLDPDATQIYGMPVFPCQGLYIGLPWIYRARYFKSGAYSVRKLHEAQEDSPR
                     TLEVQLAWSWDLINWTRAPGRPQFIPLGAPGQWDRGMIVTARAPVPVGDRLHFYYGGC
                     DGLHDDPRVNAAIGLATLRLDGFCSFRAGDTEGWLISRREPFRRPAVTLNARVGTNGC
                     VAAEILDRHNRVLPGFAKADCIPFEGDSVRHELRWRTAAFAAAPERPDYKIRFWLRNA
                     DLFSYLPADLDPAQPDADRRPATGP"
     gene            complement(6383..7909)
                     /locus_tag="PKI07_10480"
     CDS             complement(6383..7909)
                     /locus_tag="PKI07_10480"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOC51515.1"
                     /translation="MKAISLLLISTGLSLTLPAAAPPLPGNPLAGLERLKDFGTRRVS
                     SSDPNWRNGNDDRRGIAVGATLTLAELEGPGLIAHIWFTIAHRTPYYSRLLTLRIYWD
                     GEEHPSVECPVGDFFGIGHGVDKSFVSLPIRVSSEGRGRNCYWPMPFRKSARITVTNE
                     SKVPCASIYYYIDWQKHSTLPDDTAYFHACYRQEHPCVMGRNYLLADIAGRGHYVGTI
                     QSVYAMSAAWYGEGDDFFFIDGETEPSLRGTGTEDYFCDGWGFREQDGPFYGTPLWEG
                     RKAGDRCTAYRFHLTDPMVFNQSLRVEIEHKGQQIFPDGTRTVFIERDDLMSSVAFWY
                     QVEPHKPWPPLPPGPERLPFTERHLVIGGKSLAKARTAHGQLSLQELRGKVMDDKFLV
                     FQPDKDQGWVELRFTTGQTQVVDLVGNMVHGRDCGKYRALLDGQVLEVMNLHAPEPTA
                     TPHRWGMRELPAGEHVLRFEATGNPGADDGYRLGFDSFIARVPVYARPLDFDLRQIQK
                     "
     gene            8156..9673
                     /locus_tag="PKI07_10485"
     CDS             8156..9673
                     /locus_tag="PKI07_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOC51516.1"
                     /translation="MPATIGFDNERYLEQQSAAILERVQDARQKLYLEFGGKLLQDCH
                     AARVLPGFQPEVKVRLLQRLKDQAEVVLCIYAEDIEKKKMRADFGITYDTDALKTMDE
                     LREWDIAVRAVVITRFNNQPAAVAFKNKLERRGIRVYTHRATKGYPTDVETIVSPEGY
                     GANPYIETDRPIVVVTGPGPGSGKLATCLSQLYHEHQRGQPASYAKFETFPIWDRPLN
                     HEVNVAYEAATAELKDVNLIDHFHLEAYNQTAVNYNRDLEAFPLLRRIIERISGQPSR
                     YKSPTDMGVNRVGFGIVDDAVCRAAARQEIIRRYFNYACEYAMGLVERDAVERVELLM
                     RNLELRATDRRVVEPAQEAARQAQARGKGSHGFFCGAALELKDGRMVTGSNSPLLHAA
                     SSLVLNTVKLLADIPRAIHLLPPNVTRSLSHFKTNVLKGKVTSLDLEETLIALSISAL
                     MNPAAEAAIEHLKDLNGCNVHLTHIPSPGDATGLRKLGLNATSQPEFASKHLFAS"
     gene            complement(9697..13398)
                     /locus_tag="PKI07_10490"
     CDS             complement(9697..13398)
                     /locus_tag="PKI07_10490"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOC51517.1"
                     /translation="MRTRLCLSFSISLAVFLHLPGAACAEGPSSASAKLPEAAGIEYL
                     GDRMELIFQASQAWGELGKNAAAHPPGGAGAPLQIAGQTYARGLGHHAFGAITILLEG
                     QYAAFDAEVGLQPCGGGGSVCFRVFVNGQLRFDSGILRATDAARPVHVELSGAQELRL
                     EAGDGGDGIACDMANWANARLTRSHTAVRLPPEPAVDMAPFARVVTWDPNRREGARAS
                     RIEEFRAEDLFLETDLAARPDGAYCVPVSTNGLGCIGLQWLNRRALKELTLEFAAPAQ
                     APAPNSVHVQGWQGESIWQGAWEPLAGELQADGRRWVFRLPPGPGLVQTHKVRWIFPA
                     SEPAVVRPPGAFTRTRWQTVPLRVQAEKPVKGGTLTVYNGEVLSVAGRAPGRVQASRE
                     SEGLDARGCAPATLALNLARPLHLAVRSSRPSLLHSDPTVLQFRLPTGSFGVAVEDLL
                     THDGVYLPDHGLYITRDPAPLTLAGYRRKIAAQKSILPQVRELPEQTLAQAMARTHHS
                     AQREGPVMLSLACDNAKFVLEREGTLRFQTGPAPDSDWFATAGEIQPQFGAGPPAALT
                     RRRDGGWLPIPVITVSHAGVQYRERVLVAPADAPGDHPARLNRRSVCVAEFVLTNTLA
                     QPAQASLQLNCLSAGRPRKAARLAPGPGGYYAADEGGPFALAATAAVSPLEVSASDGQ
                     LRLAGTLPAHAAVSLAVLIPAQRADLAARLDPARLRADTAAYWDAVLAPAMQVQTPDP
                     FLNEVIRASQVRCLIAARNEAGGARIAPWIAAMSYGPLESEANAVIRGMDFMGHGEFA
                     RRSHDFFIHRYNTNGFLTTGYTTFGTAWQLWTLGEHYQLHRDANWLSRVAPEILRVTD
                     WIIRQTEKTRRPAAGPASPGAAGNPEYGLMPPGVMADWNSFACHFAMNAYYYAALREV
                     GHAIADLPADRGGRRSAYLLDRAAELRANLLRAYRWTQAQSPVLPLRDGTWIPHYPSQ
                     VHSPGKLADFFPGQDAGRSWCYDVELGAHQLIPTAVLDPRSREAGRMLDHMEDVQFLA
                     EGWFDYPAAQNALDWFNLGGFSKVQPYYTRNCEVYALRDDVKPFLRSYCNALASLLNP
                     EVLTFWEHFHHSGAWDKTHETGYFLHQTRTLLVTERGRELWLAPFTPAHWLADGQALT
                     VRNAPTRFGPVSYTLRSRLADGWVHATIQPPEREAPHRIVLRLRTPDGSAIRSVRVNG
                     RRHHDFAPAEGLVRLSPSRAPLDLEIRLR"
     gene            complement(13510..>13734)
                     /locus_tag="PKI07_10495"
     CDS             complement(13510..>13734)
                     /locus_tag="PKI07_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51518.1"
                     /translation="NPLSGNHDNQFAELDGHNLILRCRAPSKYAQGTGLSAFLLNGPI
                     LRKNRMENSRFTLFLTLSLPTLTIKPLTGA"
BASE COUNT         2163 a         4465 c         4934 g         2172 t
ORIGIN      
        1 ataaataccc ttgcctttcc agcagggggg cggtagtgaa acaggagcaa gccgctgtgc
       61 tttatgtctg acgttctcca tcgtttggct gcgttgcttc cggcggaccg gttgctgacc
      121 gggccggcgc agttggcggc ttacgagagc gacggtttga cggcctatcg cacgcgcccg
      181 caggccgttg cgattccgga gacgacggac gaggtggtgg cgctggtcag gttctgccac
      241 caggaaaagc tgccttttgt cgcgcgcggc agcggcacca gcctctccgg cgggtcgctt
      301 cccgtcgccg atggcatcgt catcgcgctt aaccgcctga cccggattct gcggcttgac
      361 ccggcccaac ggattgccgt tgtcgagccg ggagtggtca atacgcaggt gtcggtggcg
      421 gcggcggcgc atcggctgca ttatgcgccc gatccgtcca gcgccctgat ttgcaccatt
      481 gggggcaatg tggcctttaa ttccggcggc gcccattgcc tcaagtatgg catgacggcc
      541 aaccatatcc tgggcctcaa agcggtcctg cccacgggcg aggttgtcga gtggggcggc
      601 gccagccgcg agcacattgg gcccgattgg tgcggattgt tcaatggcag cgaggggctt
      661 ttcggcatcg cgctggagat caccctgcaa ctgctgccgc gggccgagtg tttttacacc
      721 gtgctggcgg gctaccggac gctggagcag gcgggcgacg cggtgtcggc ggtggtggcc
      781 agcggcctgc tgcccggcgc gctcgaaatc atggatgcgc tggccctcga agccgccacc
      841 gccgcggtgc atgccgagta tccccccggc gccgaggcgg tgctgatcgt cgaactcgag
      901 ggcacgcgcg aggccgtcgc cgcggaccgg ggccggctcg aggccattat tgccggcagc
      961 cagccgatcg agatgcgccc cgcgcgcgat gcggaggagc ggctggcgat ctggaagggg
     1021 cgcaaaagcg ccttcagcgc ggtgggccgg ctgtcgcccg atttcattgt ccaggacggc
     1081 gtggtgccgc ggcggcggct gggcgaggct ctccgccaca atggcgagct ggcgcgcgcg
     1141 gcgggcttgc gcgtcgccaa tgtcttccac gccggcgatg gcaatcttca tccgctgatt
     1201 ctctttgacg gccgcgaggc gggggcgctg gaacgcgccg aggccctggc cggccgcatc
     1261 ctcaagcgct gcgtcgaaat gggcggctcc atcacgggcg agcatggcgt gggggtggaa
     1321 aaactcgaat acctcccggc gatgtataat gcggacgagc tggactgcat gatgcgcctg
     1381 cgcgccgcct ttgatccgct gggcattgcc aaccccggca agaagttccc gcgcgccggc
     1441 gcgcccgccc tgtcgcagcg cgggctgcat cccctggagc gggcgggggt gatttcccgc
     1501 gagtgaggcc catgaacgag ccgttgacac ccgcaacgcc cgaggcgctg gccgaagccg
     1561 tccgcgcgca tccgcgggtg ctggcgatcg gcgcgcgcac caagccgcgc ctttcccgga
     1621 tgcccgccga ggtgacgctg atctccacgc gcgccctgcg cggcatggtc gaatacgagc
     1681 cggatgagtt cacctttacc gcccgggcgg gcacgcccgt gcgcgagatt gagcgggagc
     1741 tggccgcccg gggacaatac ctgccgttcg atcccctgtg gcttgacgcc ggggccacgc
     1801 tgggcggcac cgtggccgcg ggcctcagcg ggccggggcg gtttcgcttt ggcggcgtgc
     1861 gcgatttcat cctgggcatc cgttttgtgg atggcatggg gcggttgctg cgcatggggg
     1921 gcaaggtggt gaagaactgc gccgggtttg acctgcccaa gttcatggtg ggaggcctcg
     1981 ggcgctacgg cgcgctggcc gagctttcgt tcaaagtctt cccccttccc gccgcgcgcc
     2041 tgaccctgca gttgcccacg gagggcgccg aggccgccgc gcgcgtgttc accgaagccg
     2101 ccggcgcgcg ctgggaatgc gaggcgctgg atttgctgcc cgacgggcag accgtctgcc
     2161 tgcgcctggc cgggccggcg ccggccatcg aggcggtctc ccgcgagata ctggcgcgct
     2221 ggcccggcca ggcgctggcg ccggacgggg cggaggccct gtgggcggag gtgcgcgagt
     2281 tccgctgggc gcatgcggac ggggtgctgg ccaaagtggc gctcacgccc gcggccatgc
     2341 ccgccctgga tgccgccgtc cgctcgctca agggcgcgcg cctccacttc agcgccggcg
     2401 gcaacctggc ctttgtctcg ctgcctcccg cggtggcgct gacggccctt ccggaacggc
     2461 tgcgcgcgct gggcctggcc ggcctgaccc tgcgcggcgg cgcgccgctc tggccgggcc
     2521 ggcacacgat tccggccatc gccggggccg tcaagcaggc gctggacccc gacaaccggt
     2581 ttccccccac ggaggactga tgctgcactc cattcaaccc gagcgatacg cgcctttcgg
     2641 cgaggcgatg acgcgcgcgg tacaggcctg cgtgcattgc ggcttctgcc tttcggtgtg
     2701 cccgacgtat gccgagctgc gcgaggaggc ggacagcccg cgcgggcgca tcgtgctgat
     2761 gaagctggtg ctggagggcg cgctcgcctg ggaggcggcg cagccgcaca ttgaccgctg
     2821 cctgggctgc ctcgcctgcg aacccgcctg cccgagcggc gtgccctacc gcgagctgat
     2881 cagcccgttc cgcgccctgg cggccggcaa gttccatcgc tccggcgccg accacctgcg
     2941 ccgctggctg gcggcgaaga ccatcccgca tcccgcgcgc ttccggctgg cggtccgctc
     3001 ggcgggcgcg ggccgcgccc tgcgtccgct ggtgccgcgc gcgctggaat ccatgctcga
     3061 cctggcgccg cgctgcgtgc ccccggccca gacctggccc gccgtcaccc ccgcgcgcgg
     3121 cgagcggcgg gcccgcgtgg ccctgctgac cggctgcgcg cagcaggtgc tggatcccga
     3181 catcaacacc gcgacgctcg aggtgctggc ccgcaacgga gtggaggtcc tcattcccgg
     3241 ccgccaatgc tgctgtggcg ggctggcctg gcataccggc gattggccgg ccgcccgcga
     3301 cttcgcgcgc cgcaacctgg ccgctttccc ggacgatgtg gacgccatca tcaccaacgc
     3361 cgccggttgc ggatccaccc tgcacgaata ccccctgatc ctccgcggca cggcggacga
     3421 ggcccgcgcc gaggccttcg cccggcgggt ggtggacgtg tcggtttttc tgacccggct
     3481 cgggctgcgc gagaagcccg ccggcaccgg ccgcccgcag cggattgcct atcatgacgc
     3541 ctgccacctg gcccaggccc aggacatccg gagcgagccg cgcgatctgc tgcgggccat
     3601 ccccggagtg aagctgctgg aattgccgga tacgcatctc tgctgcggca gcgccggctc
     3661 ctataacctt gaccagcccg ccattgccgc cgccctgggg gagaagaagg cgcgggcggt
     3721 gctggcttcc ggcgcggagg tgctggcctc cggcaacatc gggtgcctca cccagctccg
     3781 cgcgcacctg gcccggctcg gcgccgagct tcccatccgc cacaccatcc aggtgctgcg
     3841 ggacgcctat cagccggagc cgcgttaacc ggcggccggg ccggggtttg cccgggcgcc
     3901 gccgcgcgcg ccggcgcggg ttttcccctc aggttcccgg tttgcttcgg gccgtcccgc
     3961 tccctttttc gtgctcggcg cgcagccgcc ggaatacgcg catgaaggcc tccagccgct
     4021 tcggctcgag atcgcccagg aagcggcccc cctgccggaa ggtcgagccg acgatgaagc
     4081 cgtcggcccg ggggaaatag gcggcgaggt tctccggcgt gatgccggag ccaatcagca
     4141 cggggacgcg cgcatgctcc tggacgcgct gcaattccgc cagcgcgggc ggttcggtcg
     4201 tgcgcgcgcc ggtgacgatc acgccatcca cgaggaagaa ctcggcctgc ttgacttcat
     4261 cgaggatgtc gaggtcgccg gtcagcgcgt gggaacaatg cttcttcttc acgtcgccga
     4321 agattttgat ctgctcgacc cccagctccc ggcgccggcg caggaggcgg cccgcgcagc
     4381 cttcgatcag gcccgccccg cccacgtggg cgaagacgta gccctcgacg cgcaggaaat
     4441 cgaggccggc cgtgccggcg atttccagcg cggtctcgtt ggcggcttcg agcatctgga
     4501 tgccgatcgg gccggggaac tggcggcgca ccgcgtcggc gacctgctgc atcaccttga
     4561 tggcgcgcgg cgggagcggc ggtttgatgt agggcaggtc gtggctgttt tccagcacga
     4621 gggcatccac cccggcggcg tggtaatgcg ccacgtcgga cagggcttgc gcgataatcc
     4681 gcgcgtcgtc gccgccgtag cgcggcgcgc cgggcagcgg caggaccgcg atcatggcgc
     4741 cgaggaattt gtcgggaagg ttgcgcatgg gcgggggtca ggggccggtg gcgggccggc
     4801 ggtcggcgtc gggttgcgcg gggtcgaggt cggcgggcag gtaggagaaa aggtcggcat
     4861 tccggagcca gaaacggatt ttgtagtccg gccgctccgg cgcggcggcg aacgcggccg
     4921 tccgccatcg cagttcgtgg cggacggagt cgccctcgaa ggggatgcag tcggctttgg
     4981 caaagcccgg cagcacccgg ttgtgccggt cgaggatttc cgccgcgacg cagccgttcg
     5041 ttccgacgcg ggcgttgagg gtcaccgccg ggcggcggaa cggttcgcgg cggctgatca
     5101 gccagccctc ggtatcgccg gcgcggaagg agcagaagcc gtccaggcgc agcgtcgcca
     5161 gcccgatggc ggcgttgacg cgcgggtcat cgtgcaaacc gtcgcagccg ccgtagtaga
     5221 aatggagccg gtcgccgacg ggcaccgggg cgcgggcggt gacgatcatg ccgcggtccc
     5281 actggccggg cgcgccaagc gggatgaact gcgggcggcc cggcgcgcgc gtccagttga
     5341 tcaggtccca gctccaggcg agctgcacct ccagggtgcg cggggaatcc tcctgcgcct
     5401 cgtggagttt gcggacggag taggccccgg acttgaagta gcgcgcgcgg taaatccagg
     5461 gcaggccgat gtagaggccc tggcagggga aaacgggcat gccgtaaatc tgggtggcat
     5521 ccgggtcgag gtcgtcggcg gcgaacagcg ggccgtcgta gggcttggtc caggccaggc
     5581 cgtccgggga ccaggcgatg ccgacggcgc ggccgcggcg gctgtgggtt ttccaggttg
     5641 cggtgtagcg tttctggccg gggtcgtaga agaagttgac gacatcggag gagctgaaca
     5701 ggggtttctt ctccgtctcg ggaacgtatt tccagcgccg cccgtcgggg gagaaagcga
     5761 cgcgggcatg gccggcacgg ggatcgaagc cgaacagggc gtagcgctgg tggggcgccg
     5821 caggctcggg acagcggagg acgccggggt tatgacattc cccgcccgtc acctcgggct
     5881 gcgccgccga gacgcaaagg ttattggctt tcgagccgtt gaattcgatg aggcccaggg
     5941 cgggcttggt ccagtgaatc ccgtcggccg actccgcgta gccgacgtgg ttgccccggt
     6001 tgagaatctg gtaccacagg cgcagcttgc cgccgtccca ggccgcggtg ccatagacgt
     6061 aggggccggt aatggccgcg ggcacgcttt cccagggctg gtcgcctttg agcacggggt
     6121 tggcgggatg cttttcggcg gcgtgaaaga cccgggtcag gccctgttgc tcctcgacag
     6181 cccaggcgtc caggaacagg cggcgccagg ggccgcgtgc cgggccggcc agcgcggtct
     6241 cgaagggaag cggggaagga tcatccgcgg cggccggctg cgcgcgcgcg ccgccgcccc
     6301 gcggccaggc cggggcgctc gcggcggcgg tggccatcag ttgcaggaaa cggcggcggg
     6361 gcagggccgg cggggtcata agttatttct gaatctggcg gaggtcgaaa tcgagcggcc
     6421 gcgcatacac cggcacgcgc gcaatgaacg aatcgaagcc caggcggtag ccatcatcag
     6481 ccccggggtt gcccgtggct tcaaaccgca ggacatgctc gcccgccggc agctcgcgca
     6541 tgccccagcg atgcggcgtg gcggtcggtt cgggggcgtg caggttcatc acctccagca
     6601 cctggccgtc aagcagcgcg cggtatttgc cgcaatcgcg cccatgcacc atgtttccga
     6661 ccaggtccac cacctgggtc tggccggtcg tgaagcgcag ttccacccat ccctggtctt
     6721 tgtccggttg gaaaacgaga aacttgtcgt ccatgacctt gccgcgcagt tcctggaggc
     6781 ttaattggcc gtgggccgtt cgcgccttgg cgagcgattt tccgccgatg accaggtgcc
     6841 gctcggtgaa gggcagccgc tccgggccgg ggggcagcgg gggccagggt ttatgcggct
     6901 cgacctggta ccagaacgcc acggaggaca tgagatcatc gcgctcgatg aagacggtgc
     6961 gcgtgccgtc ggggaatatc tgctggccct tatgctcgat ttcgacccgc agcgactggt
     7021 tgaacaccat cgggtcggtc aggtggaagc ggtaggcggt gcagcggtcg ccggctttgc
     7081 ggccttccca cagcggcgtg ccatagaagg ggccatcctg ctcgcggaag ccccagccgt
     7141 cgcagaagta atcctccgtg cccgtcccgc gcaggctggg ctcggtctcg ccgtcaatga
     7201 agaagaagtc gtccccctcg ccataccagg cggcggacat ggcgtacacg ctctggatcg
     7261 tgccgacgta atggccgcgg cccgcgatgt ccgccagcag gtaattgcgt cccatcacgc
     7321 agggatgctc ctggcggtag caggcatgga aatacgcggt gtcgtccggc agcgtggaat
     7381 gtttctgcca gtcaatgtag tagtaaatgg aggcgcacgg gaccttgctt tcgttggtga
     7441 cggtgatgcg ggcggacttg cggaagggca tgggccagta gcagttgcgc ccgcggccct
     7501 cggacgaaac gcgaatcggg agggagacaa aagatttgtc caccccgtgg ccgataccga
     7561 agaagtcgcc caccgggcat tcgacgctcg ggtgttcctc gccgtcccag taaatccgca
     7621 gcgtcagcaa gcgggaatag tacggcgtcc ggtgggcgat ggtgaaccag atgtgggcga
     7681 tgagccccgg cccctccagt tccgccagcg tcagcgtcgc gccgacggca atgccccgcc
     7741 ggtcgtcgtt gccgttgcgc cagttggggt cggacgagga aacgcgccgg gtgccaaagt
     7801 ctttgagccg ctccagcccc gccaggggat ttccgggcag tggcggggcc gccgcgggaa
     7861 gcgtcaggct gaggccggtg ctgatcagga gcagggaaat ggctttcata tcaatcagga
     7921 aggaggcatt gttttgagat ttcggcgcgc aatcatcagg ccccagccta acctttcgcg
     7981 gccccgttgt ccatgcggca atggacggcc gccttcgccc gcgccggggc cgggggcggg
     8041 ggaaaaaggc gggaacccgg agcgcgccct tcggcgggct tcaagatgcc gctttccaac
     8101 gcggaggccc ggctgtaagc tggcggcact aatgagctaa ccaaaacgac tattcatgcc
     8161 cgcaaccatc ggattcgaca acgagcgtta tctcgaacag caaagcgccg cgatcctcga
     8221 acgcgtccag gacgcccggc agaagcttta cctggagttc ggcggcaaac tgctgcagga
     8281 ctgccacgcg gcgcgggtgc tgcccggctt ccagcccgag gtcaaggtgc ggctgctgca
     8341 gcggctcaag gaccaggcgg aagtggtgct gtgcatctac gcggaggata tcgagaagaa
     8401 gaagatgcgg gccgatttcg ggatcaccta cgacacggac gcgctcaaga ccatggacga
     8461 gctgcgggag tgggacatcg cggtgcgggc ggtggtcatc acgcggttca acaaccagcc
     8521 cgcggcggtg gcgttcaaga acaagctgga gcgccgcggc atccgcgtct atacgcaccg
     8581 cgccaccaag ggctatccca ccgatgtgga gaccatcgtc agcccggagg ggtatggcgc
     8641 caatccatac atcgaaaccg accggcccat cgtggtggtc accggccccg gcccgggcag
     8701 cggcaagctg gccacctgcc tgtcgcagct ttaccacgag catcaacggg gacagccggc
     8761 cagctatgcg aagttcgaaa cgttccccat ctgggatcgg cccttgaatc acgaggtcaa
     8821 cgtcgcgtat gaggccgcca cggcggagtt aaaggacgtg aacctgattg atcattttca
     8881 cctcgaggcc tacaaccaga cggcggtcaa ttacaaccgc gacctcgagg ccttcccgct
     8941 gctgcggcgc atcatcgagc gcatcagcgg ccagccgtcg cgttataaat ccccgacgga
     9001 catgggcgtg aaccgcgtcg ggttcggcat cgtggacgat gccgtctgcc gggcggcggc
     9061 gcggcaggag atcatccggc gctatttcaa ctacgcctgc gaatacgcga tggggctggt
     9121 ggagcgcgat gccgtggagc gggtggaact gcttatgcgc aacctggagc tgcgcgccac
     9181 ggatcgccgc gtggtggagc cggcccagga ggcggcgcgc caggcgcagg cccggggcaa
     9241 gggcagccac ggcttctttt gcggcgccgc cctcgagttg aaggatgggc gaatggtcac
     9301 gggctcgaac tcgccgctgc tgcacgccgc ctcgagcctg gtgctcaaca cggtaaagct
     9361 gctggccgac atcccccggg ccattcacct gctgccgccc aacgtgaccc gctcgctcag
     9421 ccattttaaa accaacgtcc tcaaaggcaa agtcaccagc ctggacctcg aggaaaccct
     9481 cattgcgctg agcatcagcg ccctgatgaa cccggcggcg gaggcggcca tcgaacacct
     9541 caaagacctc aacggctgca acgtccacct cacgcatatt ccctcgcccg gagacgccac
     9601 gggcctgcgc aagctgggcc tcaacgccac cagccagccc gagttcgcct cgaaacacct
     9661 gtttgcgagc tgacgccgcg ccgcgggccg gcgcgctcag cggaggcgga tttccaggtc
     9721 gagcggcgcc cgggaaggcg agagccgcac caggccttcc gccggggcga agtcgtggtg
     9781 ccgccggcca ttgacgcgga cggagcggat ggcgctgccg tccggggtgc gcaggcgcag
     9841 cacgatccgg tggggcgcct cgcgttcagg gggctggatg gtcgcgtgca cccagccgtc
     9901 ggccagccgc gagcggaggg tgtagctgac ggggccgaag cgggtggggg cgttgcggac
     9961 ggtcagcgcc tggccatccg cgagccagtg ggcgggggtg aacggcgcga gccacagctc
    10021 ccggccgcgc tcggtcacca gcaaggtgcg cgtctggtgc aggaagtagc cggtctcgtg
    10081 cgttttatcc caggcgcccg agtggtggaa atgctcccag aaggtcagca cctcggggtt
    10141 gaggagggag gcaagggcgt tgcagtagga gcggaggaaa ggctttacat cgtcgcgcaa
    10201 agcatagact tcgcaattac gggtgtagta aggctgcacc ttggaaaagc cgcccaggtt
    10261 gaaccagtcc agcgcgttct gcgcggccgg gtagtcgaac cagccctcgg cgaggaattg
    10321 aacgtcctcc atgtgatcca gcatgcgccc ggcctcccgg ctgcggggat ccagcaccgc
    10381 ggtggggatg agttgatggg cgccgagctc gacatcgtag caccagctcc ggcccgcgtc
    10441 ctggcccggg aagaagtcag cgagcttgcc ggggctgtgg acctgggaag ggtagtgcgg
    10501 aatccaggtg ccgtcgcgca ggggcagcac cggcgactgc gcttgggtcc agcgataggc
    10561 gcggagaagg ttggcgcgga gctcggcggc gcggtcgagc aggtaagcgc tgcgccggcc
    10621 gccgcggtcg gcgggaaggt cggcaatggc gtgcccgacc tcgcgcaggg cggcataata
    10681 gtaagcgttc atcgcgaagt ggcaggcaaa gctgttccaa tccgccatca cgccgggcgg
    10741 catgaggccg tattcgggat tccccgccgc gccggggctg gcggggcccg ccgccgggcg
    10801 ccgggtcttc tccgtctgcc ggataatcca gtcggtgacg cgcaggattt cgggagcgac
    10861 ccgggagagc cagttggcat cgcggtgaag ctggtaatgc tcgccgagcg tccaaagctg
    10921 ccaggcggtt ccgaaggtgg tgtagccggt ggtgagaaaa ccgttggtgt tgtagcggtg
    10981 aatgaagaag tcgtggctgc gccgggcaaa ctcgccgtgc cccatgaaat ccatgccgcg
    11041 gatgaccgcg ttggcctcgc tttcgagcgg gccgtagctc atggccgcaa tccacggcgc
    11101 aatgcgcgcg ccgccggctt cgttgcgggc ggcgatcagg caccgcacct gcgaggcgcg
    11161 aatcacttca ttcaggaacg ggtcgggcgt ctggacctgc atcgcgggcg cgagcacggc
    11221 gtcccagtag gccgcggtgt ccgcgcgcag ccgggcggga tccagccgcg ccgcgaggtc
    11281 ggcgcgctgg gccgggatca ggacggccag ggaaaccgcc gcgtgcgcgg gcagcgtgcc
    11341 ggcgagccgg agctggccgt ccgacgccga gacttccagc ggcgacacgg ccgcggtggc
    11401 cgccagcgca aacgggccgc cctcgtcggc cgcatagtaa ccgccggggc cgggagccag
    11461 gcgggcggct ttccgcggcc ggccggcgct caggcagttg agctggagcg acgcctgggc
    11521 cggctgggcg agcgtgttgg tgaggacgaa ttcggcgacg cagacggagc ggcggttcaa
    11581 ccgggcgggg tggtctcccg gcgcatcggc gggcgccacc aggacgcgct cgcggtactg
    11641 cacgcccgcg tgggagacgg tgatgacggg aatcggcagc cagcctccgt cccggcggcg
    11701 cgtgagcgcc gccggcggcc cggcgccgaa ctgcggttgg atttctccgg cggtggcaaa
    11761 ccagtcggag tccggcgccg ggccggtttg gaagcgcaag gtgccctcgc gttcgagaac
    11821 gaacttggca ttgtcgcagg cgagcgagag catgaccggg ccttcgcgct gggcggaatg
    11881 atgcgtgcgg gccatggctt gcgcgagggt ctgctcgggc agctcgcgca cttgcgggag
    11941 gatggatttt tgggcggcga tcttgcgccg atagccggcc agcgtcaggg gcgccggatc
    12001 gcgcgtgatg taaaggccgt ggtcgggaag atagaccccg tcatgggtga gcaaatcttc
    12061 caccgccacc ccaaacgatc ccgtgggcag gcggaactgg agcacggtgg gatcggaatg
    12121 gagcagcgag ggccggctgg aacgaacggc caggtggagc ggccgggcga ggttcaaggc
    12181 gagggtggcg ggggcgcatc cccgcgcatc caggccttcg ctttcccggg aagcctggac
    12241 gcgcccgggc gcccgcccgg ccacggaaag cacttccccg ttgtacacag tgagggtgcc
    12301 gcctttcacc ggcttttcgg cctgcacccg caagggcacc gtctgccagc gcgtgcgggt
    12361 aaaggcgccg ggcggccgga cgacggccgg ctcactcgcc ggaaaaatcc agcggacttt
    12421 atgcgtctgg accaggccgg ggcccggcgg cagacgaaag acccaccggc gtccatcggc
    12481 ctgcaactcg cccgcgagcg gttcccaggc gccttgccag attgactcgc cctgccagcc
    12541 ctgcacgtga acagagttgg gggccggggc ttgcgcggga gccgcgaatt cgagcgtcaa
    12601 ttccttgagg gcgcggcggt tgagccactg gaggccaatg cagcccaggc cgttcgtgct
    12661 gacgggcacg cagtaagcgc cgtccgggcg cgcggccagg tcggtttcga ggaaaaggtc
    12721 ttcagcgcgg aactcttcga tgcggctggc ccgcgcgccc tcccggcgat tcgggtccca
    12781 cgtcaccacc cgcgcaaagg gcgccatatc caccgccggt tccggcggca ggcggacggc
    12841 ggtgtggctg cgggtcaggc gcgcgttggc ccagttggcc atgtcgcagg cgatgccgtc
    12901 gccgccatcc ccggcttcaa gccgcaattc ctgcgccccg ctcaattcga catgcaccgg
    12961 gcgggcggca tcggtcgcgc gcaagatgcc gctgtcaaac cgcaactggc cgttcacgaa
    13021 aacgcggaag cagacactgc caccgccgcc gcaaggttgc agtcccacct cggcatcaaa
    13081 cgcggcatat tggccttcga gcaggatggt aatagccccg aaagcatgat gtcccaggcc
    13141 ccgggcgtaa gtctgcccgg cgatttggag cggcgcgccg gctccaccgg gcggatgcgc
    13201 ggcagcattt ttccccaact caccccaggc ctggctggcc tgaaaaatca actccatccg
    13261 gtcgcccagg tattcaatgc cggccgcctc gggcagtttg gcggacgcag aggacggtcc
    13321 ctcagcgcag gctgcgcccg gcagatggag aaaaacggcg agggaaatcg aaaaggacag
    13381 gcatagccgc gtgcgcatga gacattcttt aacgccatat agctctccag gcgagataat
    13441 tatggccacg atcttcaagt cacgctcctc ggatcctata ttgtccagcc gtctggattt
    13501 attttcaatt taagcacccg ttagtggctt tatcgttaac gttggcaagc taagcgttaa
    13561 aaacagggta aatctgctat tttccatgcg atttttcctc aaaatcggac catttaaaag
    13621 aaaggcgctt aggccagtac cttgggcata ctttgatggt gccctgcatc tcaaaatcaa
    13681 gttgtgtccg tcaagttcag caaattggtt atcatggttt ccactcaagg ggtt
//
LOCUS       DAOPQG010000094        13656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5255_length_13656_cov_14.835233, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000094 DAOPQG010000000
VERSION     DAOPQG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13656
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5255_length_13656_cov_14.835233"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..782
                     /locus_tag="PKI07_10500"
     CDS             99..782
                     /locus_tag="PKI07_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOC51519.1"
                     /translation="MLKASINALWQWYAGALKSGGYWVVGLMMAIESSIVPLPSEIVI
                     PPAAHLAWNDGLVFLGLPLTGWTAQAGLVVAGAVGSWLGATVMYWASRLAGRPLVMRY
                     GRYVFIPPAKIEGAERWAATYGAFGIFASRLLPVVRHLIGIPAGIVRMDYLQFSIYTL
                     FGSAIWSGILCWLGVKVGANISHGEMHKVTFWLVGFLGVVGVLYYVFVHRHMKSADSP
                     APAARADGR"
     gene            complement(783..1037)
                     /locus_tag="PKI07_10505"
     CDS             complement(783..1037)
                     /locus_tag="PKI07_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51520.1"
                     /translation="MGLPDQPISLTPPQIAELHRQLTKMRHDINNHLSLVTASLELVR
                     YRPEKSERMLATVAEQPPRIAEALLRFSAEFERALGITAP"
     gene            1326..2252
                     /locus_tag="PKI07_10510"
     CDS             1326..2252
                     /locus_tag="PKI07_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC51521.1"
                     /translation="MYSLSTCWNSHRHTDGRQMLREIRDLGFDYAELGHCTRISLLPG
                     ILEAVAAGEIRISSVHNFCPLPLGVNHAAPNLYQFSAERPRERELARRYTLKTIEFAA
                     RVKAAAVVLHCGSLELKNYTDKLLELAPRREKVADKYARLCEEVDTRREARKGPFMER
                     LSALLKELGPAAEAHGVKLGLENRQALEELPLDSDFSFLFRELASPAFVYWHDTGHAQ
                     IKENLGFLHHAMHVESLRDRLFGLHLHDVQFPGRDHCAPGTGTLDFAALKPFIRPEQI
                     KVFELNPNLPVAEVRRGIEHLKGIWGEDSPAG"
     gene            2278..3630
                     /locus_tag="PKI07_10515"
     CDS             2278..3630
                     /locus_tag="PKI07_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOC51522.1"
                     /translation="MSAPPARILRRAARDIARRLQEAGYPAFWVGGCVRDALLGRPPG
                     DYDLATAARPEQIERLFPRTLAVGRKFGVMVVLEAGCQFQVATFRAEADYQDGRRPGR
                     VAFGDARADALRRDFTVNGLFYDPVRRRLHDWVGGAADLRARVIRTIGAPAERFAEDH
                     LRLLRAVRFAAQLDFRLEAETFAAVRAHAARIRRISAERIREELVKLFQPPHAARGLE
                     LLWQSGLLEHVLPEVAATVACRQSPEFHPEGSVFNHLCRMLHHLPPAPDPALPWAVLL
                     HDVAKPLTATTDPATGSIHFYGHERLGAEMAGVILRRLRFPRKHIETVVQAVRRHMQF
                     KDAPQMRKATLRRLLLRPTFPLELELHRLDCLGSHGRLEVYDYLVAQAGLLARQPQIR
                     PPLLTGHDLIALGQPPGPALGALLAELRDQQLQGELTTRAAARQWARQRIARNRPAAP
                     "
     gene            3689..6247
                     /locus_tag="PKI07_10520"
     CDS             3689..6247
                     /locus_tag="PKI07_10520"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOC51523.1"
                     /translation="MIRHLAIRGLFLAVGLFLSGPSRAAEATAPATNALPVIKPGPVD
                     GQIASVSASMLENLHYLRQPFDEKVASRFFDRYFETLDPMRLHFTQADLAEFEPYRAN
                     LAHLTLAGKRRGEGDVTPACKIFNRYLQRVAQRAEYSEALLQREKFTFTTDERITLNR
                     KEMPYPADLEEARKLWRERVRFDYLQELLGKISARQKKPAAAAKKPAPAETNAPPPAP
                     LPPSAAGTNGSPPHSAAPPSGRGSSPTRLASPKDKDSAAGARADTDRPAVFAPVAIEG
                     IRAKADSAASAATGPAASPKKSDDEEIIETLRHRYQRNRRFFADWNNDDVLQLYLTAL
                     ARTYDPHSDYFGHAQLEQFSISMSLSLFGIGAELQSVDGYCKIRRLLPGGPAIKSKLI
                     KVNDRIVAVAQGDQPPVDIVDMSLTKAVQLIRGPKGTEVRLTIIPDGAESATRNVVSL
                     IRDEIPLTESAAKARIIEGPDANGQNLRLGVIDLPSFYATFDPQNTKDKPEPKSTTDD
                     VIKLLDKLKQEKIAGLLLDLRNNGGGSLEEAIRLTGLFVKTNSVVQVRETDGQIQEAV
                     DTDPLVAYDGPLVVLTSRHSASASEIVAGALQDYGRALIVGESSTHGKGTVQSVNQLR
                     PYIRYPVPSLTNDPGALKLTIKTFFRPSGKSTQLRGVVPDIILPAVANESKDIGESAL
                     DNPLPWSTIRSARYDRFDLVKPYLPELRERSAARIAASKDYDYVREDIALFQKQQADK
                     TLSLNLQQRLKEQQDAEARQKARERERRARPEPREKVYEITLQQAAQPGLPPPLAATN
                     ATTTATADLDSDDPDDEAAESRAPAPDAPMLEAQQILRDYLSLLPKGNLLTARN"
     gene            complement(6269..7885)
                     /locus_tag="PKI07_10525"
     CDS             complement(6269..7885)
                     /locus_tag="PKI07_10525"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC51524.1"
                     /translation="MKNPDSAPLDANPGAPAMAAGQTPKPPRPGFRLGCLLAGGAALL
                     AAASLTPARAQGDIPARPEQLTYAPLVYEPPAPEKFRVPLASGPVAYVVPDRELPLIQ
                     VVVYVRVGAYLEPAGREGLAGLAGYLLARGGTESQSAEALEERLAFLAARLESSIGPD
                     QGSVSLNLLAKDADEGLALLREVLAAPRFQEDKIALRRQQLRQDMQQRNDQSAAIEER
                     EKNFLAFGENFWANRLPTAASVDAITRADLVRFHRQWFHPANFVVAASGDFDRDQMTK
                     KLTALFADWPFAGATPPPIPTNTVFAAPGVYLVNKDVNQGRAALLLPGIQRDNPDYFA
                     VGVMNDILGGGGFTSRIMNRVRSDEGLAYDAHSRFPGGIYYPLVFEAGFQSKSRTVAY
                     AASLVLDEMKRIAAAPVSAAELNVSRRGFIERFPRIFATKAQIASTLAQDEFTGRYAR
                     QPDFWKTYRARLEALGPDDVLRVARKYLTPEKLVLLIVGQKDPVLLGHPDHPVRLAEL
                     AGGRLTELPLRDPLTLQPLPLASNPPPAAP"
     gene            complement(7882..9471)
                     /locus_tag="PKI07_10530"
     CDS             complement(7882..9471)
                     /locus_tag="PKI07_10530"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOC51525.1"
                     /translation="MKEFQAHRRRPARRGRVLARFILALALPGALPGAGQQVPVEEEH
                     LANGMRVLLVPRHDEPTVAGGWVAHVGSANERPGVTGITHLFEHMMFKGTRTIGTTNY
                     ARDLEIIAAQERVREAMRQEERQMRAAHLQGAGADPRQPESRTARYRELEKEFRRLVD
                     EQRALLVKNEFDRLYSGEGGSGMNAFTTQDLTAYFIVVPANKLELWAWMESERLLHPV
                     FREFYAERDVVLEERRLRIESTPLGKFEEQFNAMFWESSPYAWPVIGWPSDLLAISKA
                     QADEFYATYYSPQNITLILVGDFAPAAARALVRKYFERIPRGPRTPPDVVTPEVAQAA
                     EKRMYAEAETNPQVDILWHTVPFRHRDSYALEILSQILATRTGRLYKGLVLGSGRATE
                     TWAQQVSWKWEGFFNIGGEARDGHRPEQVEQEIYALLAALQREPVPPEELQKVKNHFA
                     ATAYRRLSSNYPILLHLIRNDGAGHWREINEAGPKIQAVTAADVQRVASQYFTRENRT
                     VAIYTRPAAAAPTPAATPQKP"
     gene            9724..10428
                     /locus_tag="PKI07_10535"
     CDS             9724..10428
                     /locus_tag="PKI07_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51526.1"
                     /translation="MRILALPLRLFAVLAVLGVALYPAPGQVPVAASPAGINTAFVKL
                     FGGVKAFTAKTEARVFDAQQKEAVLMRMDWAVRDGQVRLEINLEDVSGRDFPAGSATT
                     LKQAGMSRIISVFRPEKKATFMIYPGVQSYQVVPLEPAEAAASEKDLKLEKKELGRET
                     LDGHACVKNQVVVKRGQERVLTAVTWNAADLREFPVQIELKEKQNTVRLRFSRIQFAP
                     PAAAQFEVPAGYGLLK"
     gene            10425..11801
                     /locus_tag="PKI07_10540"
     CDS             10425..11801
                     /locus_tag="PKI07_10540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC51527.1"
                     /translation="MRILNLNPDCGIGASAWFVDLEGHRLLLDAGLHPRREGRAALPR
                     YELIQREELDAIAISHSHHDHVGSLPVAQRYFPQAHILTTELSYFLIERMLHNSVNVM
                     KRQRDELGLKEYPLYHHDEVDELAPRIQGFKYNREVDWAAFRKTRAGFISPTLEFFDA
                     GHTLGSAGIRVCGRKETLFYTGDVCFHDQTILRGARFAGVKADVLIMETTRGNRPLSP
                     GFTRDAEVERLSQAILRVLRRRGSILIPAFAMGRTQEILALLALLTRQGRLKPQPIYI
                     GGLGRVFTEIYDLEAHRAHRQYPNLQLRDALNLVVLGQEQVENLKLAGSRIFVITSGM
                     MSEHTASCHLARRLFADEKHAIFFVGYADPDTPGGRLKASLPGEPFLFSPLTGQVRRR
                     CEVEDFSLTAHASREDLLDFVCEVEPRAVLLTHGEADSIQWFADQIRARLPRSKVIVP
                     QPGETVEV"
     gene            12689..13627
                     /locus_tag="PKI07_10545"
     CDS             12689..13627
                     /locus_tag="PKI07_10545"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC51528.1"
                     /translation="MARVGQARRQQRHRKLRKRLAWAGGLLALVAVCLGTWYWWGLAS
                     RDHPWENSLGMKFVPVAGTTVLFGVWDVRVRDFRAFAQDRAGNGGWEYQKGSQPFILK
                     SDDWKPRGWEYGWNDPGFAQSDEHPVVCVSWEDAQAFCAWLTRKERVEGRIGAGQCYR
                     LPTDAEWSVAVGLPAESGATPREKSGKVKGVYPWGSGWPPPSGAGNCAGIEARDRDWP
                     ADWNPLEGYRDAYARTSPVGSFKANQYGLYDMGGNVWQWCEDWYDTDQKYRVLRGASW
                     INFNPDPLLSSCRSGTPADRLSFVGFRCVLEGGSPP"
BASE COUNT         2250 a         4543 c         4598 g         2265 t
ORIGIN      
        1 ggctcaggag gtaaaaaccc agggtcttgg cgggttggtc cggggcgcgt agaatctcat
       61 tccctccccg cgggttttcc gctataagct gctcgctcat gcttaaagca agtatcaacg
      121 cgctctggca gtggtatgcc ggagcgttga aaagcggcgg ctactgggtc gtgggcctga
      181 tgatggccat tgaaagctcg attgtcccgt tgcccagtga aattgtcatt ccgccggcgg
      241 cgcacctggc atggaacgac gggctggttt ttctcgggct gccgcttacc ggctggaccg
      301 cgcaagcggg gctggtggtt gccggggcgg tggggtcgtg gctgggggcg acggtcatgt
      361 attgggcgtc gcggctggcg gggcggccgc tggtgatgcg ctatggccgg tatgttttca
      421 tcccgcccgc caagattgag ggggcggaac gctgggcggc cacctacggc gcgtttggca
      481 tttttgcgtc gcggctgctg cccgtggtgc ggcacctgat cggcatcccg gcgggcatcg
      541 tgcgcatgga ttacctgcaa ttctcgatct atacgctgtt cggctcggcg atctggtcgg
      601 ggattctgtg ctggctgggg gtgaaagtcg gggccaatat cagccacggc gagatgcaca
      661 aggtcacttt ctggctggtg gggtttcttg gggtggtggg ggtgttgtat tacgttttcg
      721 tgcaccgcca catgaagagc gcggattcgc cggcgccggc ggcgcgggcg gacggccggt
      781 gatcaggggg cggtgatgcc cagggcgcgc tcgaattcgg ccgagaaccg cagcagcgcc
      841 tcggcgatgc gcgggggttg ctcggccacg gtggccagca tgcgctcgga tttctccggg
      901 cggtagcgga ccaactccag cgaggcggtg accagcgaca ggtggttgtt gatgtcgtgg
      961 cgcattttcg tcaactgacg gtgcaattcg gcgatttgcg gcggggtcag cgagattggt
     1021 tgatcgggta aacccatgcc caagaatggc accgggcccc ggccgatggc aagcgcgttc
     1081 cggtgtggcc ggttgacatg ggccggcgcc ccggtatgct ggacgaacga cccgggcggg
     1141 aatgatcggc cgcccggccc ggttggtttc cgggggacgg aaaggacgca agcaagtttt
     1201 gatcaggatg cactttaatc ggggcgaatg gggcggggcc ggcggacggc gcgaggccgg
     1261 gccgggcggc gggcgagggc gcggcgcgcg gcgcgcccgc cgggcatgat tcccggggtg
     1321 gcgctatgta ttcgctttcc acttgctgga actcgcatcg gcacaccgac ggccgccaga
     1381 tgctgcgcga gattcgcgac ctggggttcg actacgccga gttgggccac tgcacccgga
     1441 tcagcctttt gcccggcatc ctggaggcgg tggcggcggg ggagatccgg atttcgagcg
     1501 tgcataactt ttgtccgctg cccctgggcg tgaaccacgc cgcgcccaac ctctaccagt
     1561 tctccgccga gcgcccccgc gagcgtgagt tggcgcggcg ctacacgctc aagaccatcg
     1621 agtttgccgc gcgcgtcaaa gccgccgccg tcgtgctgca ctgcggcagc ctcgagctga
     1681 agaactacac cgacaagctg ctcgagctgg ccccgcgccg ggagaaggtc gcggacaagt
     1741 atgcccggct gtgcgaggag gtggacaccc ggcgcgaggc ccggaagggg ccgtttatgg
     1801 agcggctcag cgcgcttctg aaggaactcg ggccggccgc cgaggcccac ggcgtcaagc
     1861 tgggcctgga aaaccgccag gcgctggagg agctgccgct cgacagtgat ttctccttcc
     1921 tgttccgcga actggccagc cccgccttcg tctattggca tgataccggc catgcccaga
     1981 tcaaggagaa cctcggcttt ttgcaccacg cgatgcatgt cgagtcgctg cgcgaccgcc
     2041 tgttcgggct gcatctccac gatgtccaat tccccggccg ggatcactgc gcgccgggga
     2101 cgggcacact ggattttgcc gcgctcaagc cgtttatccg gccggagcag atcaaggtgt
     2161 tcgagctcaa ccccaacctg cccgtcgcgg aagtccggcg cggcatcgag catctcaagg
     2221 ggatttgggg cgaggattcg cccgccggtt gagcgtgtcc gcgcgaagcc aacggccatg
     2281 agcgcgccgc ccgcccgaat cctgcgccgc gccgcgcggg acattgcccg gcggctgcag
     2341 gaagccggct acccggcgtt ctgggtgggc ggctgcgtcc gcgatgccct gctggggcgc
     2401 ccgccgggcg attacgacct cgccaccgcc gcccggccgg agcagatcga gcggctcttc
     2461 ccgcgcaccc ttgccgtcgg gcgcaagttt ggcgtcatgg tggtgctcga ggcgggatgc
     2521 cagttccagg tggccacctt ccgcgccgaa gctgattacc aggatgggcg gcgtccgggg
     2581 cgggttgcgt ttggcgatgc ccgggcggac gcgctccgcc gcgatttcac ggtcaacggc
     2641 ctgttttatg atcccgtgcg ccggcggttg cacgattggg tgggcggcgc ggcggatctg
     2701 cgcgcccggg tcatccgcac catcggcgcg ccggcggagc gctttgccga agaccacctg
     2761 cggctgctgc gggcggtgcg gtttgccgcg caactggact tccgcctgga agcggaaacc
     2821 ttcgccgcgg tgcgggccca cgcggcccgg atccggcgca tcagcgccga gcgcattcgc
     2881 gaggagctgg tcaagctgtt ccagccgccg cacgccgcgc gcgggctgga gttgctgtgg
     2941 cagagcgggc tgctcgagca cgtcctcccg gaggtggcgg cgaccgtcgc ctgccgccaa
     3001 tccccggagt ttcatcccga aggctccgtc ttcaaccacc tttgccggat gctgcatcat
     3061 ttgccgcccg cgcccgatcc cgccctgccc tgggcggtcc tgctgcatga tgtggccaag
     3121 ccgctcaccg ccacgaccga cccggcgacc ggcagcatcc acttctacgg ccacgagcgg
     3181 ctcggggcgg agatggccgg cgtcatcctg cggcgcctcc ggtttccccg caagcacatc
     3241 gagacggtcg tgcaggcggt gcgccgccac atgcagttca aggacgctcc gcagatgcgc
     3301 aaagccaccc tgcgccgcct gctgctgcgc cccacgttcc cgctggagct ggagctgcac
     3361 cggctcgatt gcctgggttc ccatggacgg ctggaagtct atgattattt ggtggcgcaa
     3421 gccgggttgc tggcgcggca gccccaaatc cggccgccgc tgctgaccgg ccacgacctg
     3481 atcgccctgg gccagccgcc cggcccggcc ctgggggcgc tgctggccga gctgcgcgac
     3541 cagcagttgc agggcgagct caccacccgc gccgccgccc gccagtgggc ccggcagcgc
     3601 attgcccgga accgccccgc cgccccgtaa agagttttgc ggggttgcgc tccggctgtg
     3661 ctatggttaa gcatcgtaag aacacatcat gattagacac cttgccatcc gcggtctgtt
     3721 tttggctgtg gggctttttc tctccgggcc gtcgcgggcg gcggaagcga ccgcgccggc
     3781 caccaacgcc ctgcctgtca tcaaacccgg gccggtggac ggccagattg caagcgtgag
     3841 cgcctcgatg ctggagaacc ttcactactt gcgccagccc ttcgatgaaa aggtcgccag
     3901 ccggttcttt gatcgctatt tcgagaccct ggacccgatg cgcctgcatt tcacccaggc
     3961 ggacctggcg gagttcgagc cttaccgcgc caacctggct cacctgacgc tcgcggggaa
     4021 acgccgcggc gagggcgacg tcacccccgc ctgcaaaatc ttcaaccgct acctgcagcg
     4081 ggttgcccag cgggcggaat actcggaagc cctgctccaa cgggaaaagt tcaccttcac
     4141 caccgacgag cggatcacgc tgaatcgcaa ggaaatgccg tacccggcgg atttggagga
     4201 agcccggaag ctctggcgcg agcgggtgcg cttcgattat ttgcaggagc tgctcggcaa
     4261 gatcagcgcc cgccagaaga agccggccgc cgccgccaaa aaaccggcgc ccgcggaaac
     4321 caacgcgccc ccgccggccc cgctcccgcc atcggcggcc ggcacgaacg gcagccctcc
     4381 acactccgcc gcgccgccgt ccggccgggg gtcatcgccc acgcggctgg cctcgcccaa
     4441 agacaaggac tccgcggcgg gcgcgcgcgc ggacaccgac cggccggctg tattcgcgcc
     4501 ggtggcgatc gaaggcatcc gcgcgaaagc ggactccgcg gcgtccgccg ccacgggccc
     4561 ggccgcgtcc ccgaagaaga gcgacgacga ggaaatcatc gagacgctcc gccaccggta
     4621 tcagcgcaac cggcgctttt tcgccgattg gaacaacgac gacgtgctgc aactttatct
     4681 gaccgcgctg gcccgcacct atgacccgca ctcggactac ttcggccacg cgcagcttga
     4741 gcagttttcc atcagcatga gcttgagcct gttcggcatc ggcgccgagc tgcaatccgt
     4801 ggacggctac tgcaagattc gccggctgct gcccggcggg ccggccatca agagcaagct
     4861 catcaaggtc aacgaccgca ttgtcgccgt ggcgcaaggc gaccagccgc ccgtggatat
     4921 tgtggatatg agcctgacca aggcggtgca gttgatccgc ggccccaagg gcacggaagt
     4981 gcgcctgacc atcattcccg acggcgccga gtccgccacg cgcaacgtcg tgagcctgat
     5041 ccgggacgaa atcccgctga ccgagtcggc ggccaaggcc cggatcatcg aggggccgga
     5101 tgcgaacggc caaaatctcc ggctgggcgt gattgacctg ccgtcctttt acgccacctt
     5161 cgatccccaa aacaccaagg acaagccgga gcccaaaagc accaccgacg acgtcatcaa
     5221 gctcctcgac aagctgaagc aggagaagat cgccggcctc ctgctcgatt tgcgcaacaa
     5281 cggcggcggc tcgctggagg aggccatccg gctgaccggc ctgttcgtca agaccaactc
     5341 ggtggtgcag gtgcgcgaga ccgacggcca gatccaggag gccgtggaca ccgacccgct
     5401 ggtggcgtat gacggcccgc tggtggtgct gaccagccgc cacagcgcct ccgcgtccga
     5461 gattgtggcc ggcgccctgc aggactatgg ccgggcgctc atcgtcggcg aatcctcgac
     5521 gcacggcaag ggcacggtcc agagcgtcaa ccagttgcgc ccctacattc gctatcccgt
     5581 cccgtcgctc accaatgacc ccggcgccct gaagctgacc atcaagacgt tcttccggcc
     5641 cagcggcaaa tccacccagt tgcgcggcgt cgtgccggac atcatcctgc ccgccgttgc
     5701 caacgagtcc aaagacatcg gggaaagcgc gctcgacaac ccgctgccct ggagcaccat
     5761 ccgcagcgcc cgctacgacc gcttcgacct cgtcaagccc tacctgcccg agctccgcga
     5821 gcgctccgcc gcccgcatcg ccgccagcaa ggactacgat tatgtgcgcg aggacattgc
     5881 cctgttccaa aaacagcagg ccgacaagac cctttcgctg aacttgcagc agcggctcaa
     5941 ggaacagcag gatgccgagg cccgccagaa agcgcgcgag cgcgaacgcc gggcgcgccc
     6001 ggaaccgcgg gaaaaggtgt atgaaataac cctccagcag gcggcgcagc ccggcctgcc
     6061 gcccccgctc gccgccacca acgccaccac gaccgccacc gccgacctcg attccgacga
     6121 cccggacgac gaggcggcgg agtcgcgcgc gccggccccg gacgcgccca tgctcgaggc
     6181 gcagcagata ctccgggatt acctttccct gctgccgaaa ggcaatttgc tcaccgcccg
     6241 caactaaagc ccttcgccgg gcggccggct acggcgccgc cggcggcggg ttggaggcca
     6301 gcggaagcgg ctgcagggtc agcgggtcgc gcaggggcag ctccgtcagg cgcccgcccg
     6361 ccagctccgc cagccgcacc gggtgatccg ggtggccgag cagcaccggg tctttttgcc
     6421 ccacgatgag caggaccagc ttttcggggg tcagatattt ccgggccacg cgcagcacgt
     6481 cgtccgggcc cagggcctcc aggcgcgcgc ggtaggtttt ccagaagtcc ggctgccggg
     6541 cgtagcgccc ggtgaattcg tcctgagcca gggtgctggc gatctgcgcc ttggtcgcga
     6601 agatgcgcgg aaagcgctca atgaacccgc gccgggagac gttcaactcg gcggcggaga
     6661 ccggcgcggc ggcgatgcgc ttcatttcgt cgagcaccag cgacgccgca taggcgacgg
     6721 tgcgcgattt ggattggaac ccggcctcga aaaccagcgg gtagtagatg ccgcccggga
     6781 aacgggagtg cgcgtcgtag gccagcccct cgtcggaacg cacccggttc atgatgcgcg
     6841 aggtgaaccc gcccccgccg aggatatcgt tcatcacccc caccgcgaag tagtccgggt
     6901 tgtcgcgctg aatgcccggc agcagcaggg ccgcgcgccc ctggttgacg tccttgttga
     6961 ccaggtaaac gccgggggcg gcgaagaccg tgttggtcgg gatgggcggc ggcgttgccc
     7021 ccgcgaacgg ccagtccgca aacagcgccg tgagcttttt cgtcatctgg tcgcggtcga
     7081 aatcgccgct ggccgcgacc acgaagttgg cggggtgaaa ccattgccgg tggaaacgca
     7141 ccaggtcggc gcgggtgatc gcgtccacgg aggccgccgt gggcagccgg ttggcccaga
     7201 agttttcgcc aaaggccagg aagttctttt cccgctcttc aatggcggcg gactggtcgt
     7261 tgcgctgctg catgtcctgc cgcagttgct gccgccgcag cgcaatcttg tcctcctgga
     7321 aacggggcgc cgccagaacc tcgcgcagga gggccaggcc ctcgtccgca tccttggcga
     7381 gcaggttcag gctgacgctg ccctggtcgg ggccgatgct ggattccagc cgggcggcca
     7441 ggaacgccag ccgctcctcg agggcttccg cgctctgcga ctccgtgccg ccccgggcca
     7501 gcaggtagcc ggccaggccg gccagccctt cccgcccggc cggctccagg taggcgccaa
     7561 cgcggacata caccaccacc tggatcagcg gcagctcgcg atccggcacc acgtaggcca
     7621 ccggcccgct ggccagcggg acgcggaact tctccggcgc cggcggttca taaaccagcg
     7681 gcgcataggt gagctgctcg ggccgggcgg ggatgtcccc ctgcgcccgg gcgggcgtga
     7741 ggctggccgc cgccagcagc gcggcgccgc cggccaggag gcacccgagc cggaaaccgg
     7801 ggcgcggagg tttcggggtt tggccggcgg ccatcgccgg ggcgccgggg ttcgcgtcga
     7861 gcggtgcgga atccgggttc ttcatggttt ttgcggggtt gcggcggggg tgggggcggc
     7921 ggcggcgggg cgcgtgtaaa tggccaccgt ccggttctcc cgggtgaagt actggctggc
     7981 cacgcgctgc acgtcggcgg cggtgacggc ctgtatcttc ggcccggcct cgttgatttc
     8041 gcgccagtgg ccggcgccgt cgttgcggat gagatggagc agaatcgggt agttggagga
     8101 aagccgccgg taggccgtcg ccgcgaagtg gtttttcacc ttctgcaatt cctcgggcgg
     8161 caccggctcc cgctgcagcg ccgcgagcag cgcgtagatt tcctgctcca cttgctcggg
     8221 gcggtggccg tcgcgggcct cgccgccgat gttgaagaag ccttcccatt tccaggaaac
     8281 ctgctgggcc caggtctcgg tggcccggcc gctgcccagc accagcccct tgtacagccg
     8341 gccggtgcgc gtggccagaa tctggctgag gatttccagc gcatacgaat cccggtgccg
     8401 gaaaggcacg gtatgccaca ggatatccac ctgcggattc gtctcggcct cggcgtacat
     8461 gcgcttttcg gccgcctgcg ccacttccgg tgtgaccacg tccggcggcg tccgggggcc
     8521 gcgcgggatg cgctcgaagt atttgcgcac cagcgcccgg gccgcggcgg gcgcgaaatc
     8581 gcccaccagg atgagtgtga tgttctgggg cgaataatac gtggcgtaga actcatccgc
     8641 ctgcgccttg gaaatggcca gcaaatccga gggccagccg atcaccggcc aggcgtaggg
     8701 cgaggattcc cagaacatgg cgttgaactg ctcctcgaac ttgcccagcg gcgtggattc
     8761 gatccgcagc cgccgctctt caagcaccac atcccgctcg gcgtaaaact cgcggaaaac
     8821 cgggtggagc agccgctcgg actccatcca cgcccacaac tccagcttgt tggccggcac
     8881 gacaatgaaa taggccgtca ggtcctgggt ggtaaaggcg ttcatccccg aaccgccctc
     8941 gccgctatac aggcggtcaa actcgttttt aaccagcagc gcgcgctgct cgtccaccag
     9001 ccggcgaaac tccttttcca gctcgcggta gcgggcggtg cggctctcgg gctggcgcgg
     9061 atccgccccc gcgccctgca ggtgggcggc gcgcatttgc cgctcctcct gccgcatcgc
     9121 ctcgcggacc cgctcctgcg cggcgataat ctccaggtcg cgcgcgtaat tcgtggtgcc
     9181 gatcgtccgg gtgcccttga acatcatgtg ctcgaagaga tgcgtgatgc cggtcacgcc
     9241 cggccgctcg ttcgcgctgc ccacatgcgc cacccagccc cccgccaccg tcggctcgtc
     9301 gtgccgcggc accagcagca cccgcatccc gttggccagg tgctcctcct ccaccggaac
     9361 ctgctgcccc gcccccggca gcgcccccgg cagcgccagg gccaaaataa accgggccag
     9421 aacccgcccg cggcgcgccg ggcggcggcg atgtgcttga aactctttca tgctcgtgcg
     9481 cacaacctaa acggcccggg cccgcctgcc aatagcaagc cgcgcgaaaa tccccccgct
     9541 ccccgccgcg cgcgccggct tccccgggcc acccagccct tccccacccg gcccgggagg
     9601 agggcgcccg cgcggccccg ggttctcccg ccggggccgc cgcaatccgc ggggccgcgg
     9661 gccgccgcaa accttgtttg ccgaacgccg cccgggtgcg tatagtggag aaaaatcact
     9721 gccatgagaa tacttgcctt gcccctgcgg ttgtttgcgg tgctcgcggt gctcggggtc
     9781 gccctctacc ccgcgccggg tcaggtgccg gtggccgcgt cgccggcggg catcaacacg
     9841 gcctttgtga agctgtttgg cggcgtgaag gcgttcacgg ccaaaaccga ggcgcgcgtc
     9901 tttgacgccc agcaaaaaga ggcggtgctg atgcggatgg actgggccgt ccgggacggc
     9961 caggttcgcc tggagattaa cctggaggac gtgagcgggc gggattttcc ggcgggctcg
    10021 gcgaccaccc tgaagcaggc gggcatgagc cgcatcatca gcgtcttccg ccctgagaaa
    10081 aaggcgacgt ttatgattta ccccggcgtg cagtcgtatc aggtcgtccc cctggagccg
    10141 gcggaggcgg cggcgtcgga aaaggatctg aagctggaaa agaaggaact gggccgggag
    10201 accctggacg gccatgcctg cgtgaagaac caggtggtgg tcaagcgcgg ccaggagcgg
    10261 gttttgaccg ccgtcacctg gaacgccgcc gacctccggg agttccccgt gcaaatcgag
    10321 ctcaaagaaa agcagaacac cgtgcgcctg cgcttctccc gcatccagtt cgctcccccc
    10381 gccgccgcgc agttcgaggt gccggccggg tacggcttgt tgaagtgagg atcctcaacc
    10441 tcaaccccga ctgcggcatc ggcgcgtcgg cctggtttgt tgacctcgag gggcaccggc
    10501 tgctgctgga cgccggcctc cacccccgcc gcgaaggccg cgccgcgctg ccccggtacg
    10561 agttgatcca gcgcgaggaa ttggacgcca tcgccatctc gcacagccac cacgaccacg
    10621 tcgggtccct gccggtggcg cagcgctatt tcccgcaggc ccacatcctg acgaccgagt
    10681 tgagctattt cctcatcgag cgcatgctcc ataattcggt caacgtcatg aagcgccagc
    10741 gcgacgagct cgggctcaag gaatacccgc tgtaccacca cgacgaagtg gacgaactgg
    10801 cgccccgcat tcagggcttc aaatacaacc gcgaggtgga ttgggccgcg ttccgcaaga
    10861 cgcgcgccgg gttcatctcc cccaccctgg aattctttga cgccggccac accctcggct
    10921 ccgccggcat ccgggtgtgc ggccggaaag agaccctctt ctacaccggc gatgtctgct
    10981 ttcatgacca gacgattctg cggggcgccc ggttcgcggg cgtgaaggcc gacgtgctca
    11041 tcatggaaac cacgcgcggc aaccgcccgc tgtcccccgg cttcacccgc gacgccgaag
    11101 tggaacgcct cagccaggcc atcctgcgcg tcctccgccg ccgcggctcc attctcatcc
    11161 cggccttcgc gatggggcgc acgcaggaaa tcctggccct gctggccctg ctgacgcgcc
    11221 agggccgcct caaaccccag cccatctaca tcggcggcct ggggcgcgtg ttcacggaaa
    11281 tctacgacct ggaagcgcac cgcgcgcatc gccaataccc caacctgcaa ttacgcgacg
    11341 cgctgaacct ggtggtgctc ggccaggaac aggtggaaaa cctgaaactg gccggcagcc
    11401 gcatcttcgt catcacctcc ggcatgatga gcgagcacac cgcctcctgc cacctggcgc
    11461 ggcgcctgtt cgccgacgaa aaacacgcca tcttcttcgt cggctatgcc gatcccgaca
    11521 ccccgggcgg acggctcaaa gcctccctcc ccggggaacc cttcctcttc agccccctga
    11581 ccggccaggt gcgccggcgg tgcgaagtcg aggatttctc cctcaccgcc cacgccagcc
    11641 gggaagacct gctggacttt gtctgcgagg tcgaaccgcg cgccgtgctg ctcacccacg
    11701 gcgaggccga ttccatccag tggtttgccg accagattcg cgcccggctc ccccgcagta
    11761 aagtcatcgt gccccagccc ggcgaaaccg tggaagtcta accgcgcgta gccatcaatg
    11821 acccccgggc cataaatctc ccttgggagg gccttggcag catccggctc caaaacgata
    11881 ccgttgggca atccaaccgc ccacccccgc cacatcggaa cccggacgag cccaaccgcc
    11941 caagccatag ccccggcaag ccccttgttt ggcgaatcca agggcagaac cgctgaatcc
    12001 agggcctttc gccggtaaaa tcaaggattc aggcatagag aagggcaaaa caggggcaaa
    12061 accgcagtca tgccggcttc ggcttaccct gcacttaccc tgccttcagg ccgtgtacaa
    12121 ggggtgtaca agggatgctc accgggagtt catgggattt tagctggtac acccggtggg
    12181 catcccttgt acaccccttg tacacggcct gaaggcaggg tatatccacg gtatctggat
    12241 agtggctgcg gatgccggaa aaggcctgaa cggcgaggag aaacggaacg atgagtaata
    12301 agttcagtaa aaacagttag ttatgaatcc ggggctggcg ggattgggtt tttccggggc
    12361 gagctgatgc ccggatggct tttggcttcg caaatgcctt gcctgaaacg ttgccgtggc
    12421 atagttcagc cgtgcgcctc tgttccattg gagccgaaat gggcctgaca ggccagcggg
    12481 cagcctcaac gaactgtttg gctggcggat tcagcggtct ggccgcgggt ttgtaaatgt
    12541 ctttcgccta tacaaccagg gacttgctcc tttggtgaag ccgaatggaa gtggaaatct
    12601 gaatcattcc ggctaaagcc agtacggcgt ggccgtgaaa acgggcggcc aagggtgcgt
    12661 gggagctatt tcaggagggc ttgacagatt ggcacgggtt gggcaggccc ggcggcagca
    12721 gcgccaccgg aagctgagga agcggctggc gtgggccggg gggctcttgg cgctggtggc
    12781 agtgtgcctg gggacttggt attggtgggg gttagcttca cgggatcatc cgtgggagaa
    12841 cagcctgggg atgaagtttg tgccggtggc ggggacgacg gtgttgtttg gggtgtggga
    12901 tgtgcgggtg cgggacttcc gggcctttgc gcaggatcgg gcgggcaacg gcggctggga
    12961 ataccagaaa gggagccagc ctttcatcct gaagagtgac gactggaagc cgcggggctg
    13021 ggaatatggt tggaacgatc cgggttttgc gcagagcgac gagcatccgg tggtgtgtgt
    13081 gagctgggag gatgcgcagg cgttctgcgc gtggctgacg cggaaggagc gggtggaggg
    13141 ccggatagga gcgggccagt gctaccggtt gccgacggat gcggaatgga gcgtggcggt
    13201 ggggttgccg gccgaaagtg gtgcaacgcc ccgggagaag agtgggaagg tcaaaggcgt
    13261 ttatccgtgg ggcagtgggt ggccgccgcc gtctggggcg gggaactgtg cggggatcga
    13321 agcccgggac agagattggc ctgcggactg gaaccccctc gagggatatc gggacgccta
    13381 tgcgcgcacc tcgccggtgg ggagcttcaa ggccaatcag tatgggttat atgacatggg
    13441 cggcaatgtg tggcaatggt gtgaggactg gtatgacacc gaccagaaat accgcgtgtt
    13501 gcgcggggct tcgtggatca acttcaaccc cgaccctctg ttgtcctcgt gccgctccgg
    13561 cacgcccgct gatcgcctca gctttgttgg gtttcggtgt gtgttggagg gagggtcccc
    13621 tccgtaggct gggtatactt aatccttggg tgcttg
//
LOCUS       DAOPQG010000095        13362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5382_length_13362_cov_15.519426, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000095 DAOPQG010000000
VERSION     DAOPQG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13362
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5382_length_13362_cov_15.519426"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..1963
                     /locus_tag="PKI07_10550"
     CDS             395..1963
                     /locus_tag="PKI07_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51529.1"
                     /translation="MNSYFNTGDWVIILIYLLGIIALGLWFGKDQHNTRDYFLGSKNV
                     PWWGIGMSIVAAETSALTIIGVPAMAYGGNIAFIQMIIGYVIARIILAIVLVPHYFKG
                     EIYSPYQLFADTFGHSARQTAGGFFLISETLAAGVRVYVASIPVKLMLGDKVLSLGLG
                     DPIFGAIFLFVVLSLLYTYVGGVKAVIWTDAVQFALFLLGGVFALCYIPTLVEGGLGG
                     LFAKATAAGKLHWLNAAPAPGASFAQFLLDKPFNIWMGVIGGTVMVMSSHGAEQLIVQ
                     RVLACKNVADGRKALILSAVVIFPLFLIFLLVGAMLWVFYQDRPFQIPLPETSPGSGI
                     TANDFIFPIFMMTEVPHLLKGFLIVAILSAAMCSVSSALTSLASVSTMDFVKHVFPGR
                     SEAFFLRFSKISTVVWAAALILVAYLSRQVPFVLNAAFALRGLTSGALLGGLALAIFW
                     RKGRALPIIAGMLVSLGCMTAIQLLPKFALTRDWWMKTIGTEIFWPWYTLIGVVITLL
                     TAGLVRRLLPPPAR"
     gene            complement(2059..2823)
                     /locus_tag="PKI07_10555"
     CDS             complement(2059..2823)
                     /locus_tag="PKI07_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HOC51530.1"
                     /translation="MQPKIRIMPCLDMQNGRVVKGVHFVDIQDAGDPAACARAYCAAG
                     ADELAMLDITATVEHRGTLLEVVKRVAAVATVPFTVGGGIRDVASARAVLDAGAHKVS
                     TSSAAFRNPPVIAEMIRELGAEKVIVAIDVDHNPALPSGYEVFVDGGRTATGADALDW
                     ARRVADYGAQCILPTSKAADGARTGFDLPLIRGIAAATPAEVVASGGAGTMEHFREAA
                     AAGATVLLAASVFHFGLIRIPELKQYLRDHGLNVSL"
     gene            complement(2859..3791)
                     /locus_tag="PKI07_10560"
     CDS             complement(2859..3791)
                     /locus_tag="PKI07_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC51531.1"
                     /translation="MNDRTFIANQIATLRREVGRGTAINALSGGVDSAVVTVLAHRAL
                     GRQLKTIFVDNALMREGEPQLVVKTFQDLGIPVELVDARRQFLRALKGLTDPEEKRQA
                     ITDTFYRGVFGKAVKRARATFLLHGTILTDIEETVAGIKRQHNILAQLGINPQQAYGY
                     KVLEPLATLRKDGVRRVAALLGLPASVTRRIPFPGPALATRIVGEVTPARLDIVRQAT
                     AIVEAELRRTGAFQYLAVLLNDRATGIRHGRREFGQIIVVRCIDSTDARQATVRELPW
                     PTLRRLSRRITAIPGVNRCVYDLTPKPPATVEYL"
     gene            complement(3889..4344)
                     /locus_tag="PKI07_10565"
     CDS             complement(3889..4344)
                     /locus_tag="PKI07_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOC51532.1"
                     /translation="MVSTPVQELIPVLQIAIGPVILISGVGLLLLTLTNRYGRAIDRS
                     RQLLRELRAAAGPERPRLEGQVAILYRRARLLRLSITLAGVSVLLASVLIIALFLAAL
                     RPAGTAWLISLLFIACMAALIGSLAAFLRELHLSLGALKLELTAPPPPD"
     gene            complement(4446..5972)
                     /locus_tag="PKI07_10570"
     CDS             complement(4446..5972)
                     /locus_tag="PKI07_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOC51533.1"
                     /translation="MLAKVCSAAVSGIEAYPVEVEVNAGYGDTLIVIVGLPDAAVKES
                     RDRVTTALANSGFSFTFGRTTINLAPADVKKEGPSFDLPIAVGMVAASEQMESGQLDH
                     FALVGELALTGAVRPVKGVLPIALRARAQGKRGLLVPPDNAAEAAVVAGLQVIPVRNL
                     REAVSFLEGSLPISPTQVDVARIFDQAPDGELDFAEVKGQESVKRALEIAAAGGHNVL
                     LIGPPGTGKSMLAKRLPTILPPLTLEEALETTKIHSIAGLLAPGQALVTRRPFRAPHH
                     TASDAGLLGGNVNPTPGEISLAHHGVLFLDELPEFKRTVLETLRQPLEEGHVTISRAA
                     GTMTFPSQFMLVAAMNPTPDGKMPAESRSSPREIQNYLGRISGPLLDRIDLHVEVPAV
                     KFREITSPLPGETSAQIRARVLAARQRQQTRLRDKPKITCNARLGSRELKTYCALDEP
                     TMELLKFAMSDLNLSARAYDRILRVARTIADLAGADNLASEHISEAIQYRSLDRQLWT
                     "
     gene            6146..6664
                     /locus_tag="PKI07_10575"
     CDS             6146..6664
                     /locus_tag="PKI07_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51534.1"
                     /translation="MHDAPKNHVVKALRIGSRLRSCWQAVAAPGLWLLMSALPAFAQP
                     YAIDWFTLDGGGGTSTGGVYAVRGTIGQPDAGPTSLTGGPYSLSGGFWTISAVQTPGA
                     PLLSIVRTSTNTVAVFWPSPSTGWVLQQNAQNLGTPNWSNVPGAPIDNGTNRTVIVNP
                     PVGHRFYRLFKP"
     gene            6713..8314
                     /locus_tag="PKI07_10580"
     CDS             6713..8314
                     /locus_tag="PKI07_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51535.1"
                     /translation="MQAKTFIRCASLALVFLGTLGLSGAPAWAQGTAFTYQGHLASGS
                     GGANGIYDLQFTIYNASSGAGVVAGPLTASAVAVSNGLFTVVLDPGVGVFTGAERWLG
                     IAVRTNGAGAFTTLAPRQRITAAPYAVMSSNIAGTVAPASLPPGGSWALSTTLTLDAG
                     TLAIDPVNNRVGIGTSFPAYPLHLQAGQSVARLDSTANTFGSVIELRNTTAWPTYLGA
                     INFNNAAGTYPGQIGYLATNVMVFRTGGAERMRLNDSGRLDITGTYSSVIVNNINHYS
                     GLPYPTGISATSDSVFGTAIRASSDHGTALSATSSDGLAGLFSGAVQINYASPFSKPQ
                     LHLKDPSTNGFSRLRMQTGSRPFWDIAVGGDNSLRFFADGNGDVVTLQTNGQLFVKVL
                     TITGGADIVEPFQMSEPELSPGAVVVIDEANPGKLKLSSAPYDRRVAGVISGAGGVRP
                     GLCLRQEGRVEGDQPVALTGRVYVQADTVNGPIQPGDLLTTSLTPGRAMKVTDHARAQ
                     GAILGKAMTGLAAGEGLVLVLVSLQ"
     gene            8328..9374
                     /locus_tag="PKI07_10585"
     CDS             8328..9374
                     /locus_tag="PKI07_10585"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOC51536.1"
                     /translation="MTRLILQSALAGLLWLAAAAASGQTINLRMSVKVIVHPGTGAWP
                     PGITAQVFTNAVAAANEWMASYCRGYRYQLTELQAIGGPSQGGTNGPSRWYGLEFRGD
                     PLRRQFFNLATNDTRYLLRFDQINIYVASGFANPGDSGGAMPIPPGETNYIGGQIYAD
                     DGAWWIVHELGHFFGLPHTFATQPKDSCTPGDDGFTDTLLDSTCWSNQNQVAQFHFGA
                     DYDELNNNQKDQVDTVFFNVMSYHDYTNKNTTENRLTEQQLDRITDHANGDRHAFVGG
                     YTRFVSPSGNDSNSGLVNNAPKRTALNAVNASASGGGDIVLLRPGNYNERTNFNRRVT
                     LRATRAGWATIGKP"
     gene            9424..11610
                     /locus_tag="PKI07_10590"
     CDS             9424..11610
                     /locus_tag="PKI07_10590"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOC51537.1"
                     /translation="MPHSGPMAAYALTIFAGAFLLFQVQPLMGKYILPWFGGGPGVWT
                     TCLLFFQTLLVGGYAYAHGSARWLKPRAQAIVHLALLAGALALLPITPGDAWKPSGAG
                     NPTWQILRLLAACLGLPYLALAATSPLLQHWFSRTHPGTTPYRLYALSNFGSLLALLS
                     YPFFFEPHCTRLTQARLWAWGLVAYAIGCGFCAVKQAKAENRGGAAEAPPAPLAPGPP
                     PSAAPRSITRRLLWLLLPACASVLLLAVTNKLCQDVASIPFLWILPLALYLLSFIICF
                     DSPRWYRRVPYALALVGALAALGWALFNASDLPLRQQLVIYGCGLFLCCMVCHGELYR
                     LKPPPEQLTGFYLAIALGSALGGLFVAVGAPLLFTDYYELHWGLLLCGLLFVFVCLAE
                     GRLFPPPPALDRNAPGPGRRWLARLAGPWFLRGNLPRFRYGRELSGAGLLLGVIGLGA
                     VLWWETRTAAAQRVFQCRNFYGTLTVYEYAPENPESHCFLLQHGRIAHGVQFASSERA
                     ATPVSYYGPGSGIWLGMNALPPGPRRIGVAGLGAGSMAVFARAGDYLRFYEINPQVEQ
                     VAAAWFTYLTNCPATVEIATGDARLSLEHEPPQNFDLLVLDAFNSDAVPVHLLTREAF
                     AVYRRHLRPGGVIAINISNHHLNLKPVVANLARHFHCQWISIDYEETDEEWWLYSSTW
                     ALMSDDGKVINSPAVRNAASSPCDPVTPSPIPLWTDDFVSLFQILY"
     gene            complement(11607..11984)
                     /locus_tag="PKI07_10595"
     CDS             complement(11607..11984)
                     /locus_tag="PKI07_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOC51538.1"
                     /translation="MKTTMQTEQRAKAEPRHEPGYVTPEVNIYETKDGYVVEAEMPGV
                     HKDGLEITLEDNEITLVGRRQNETVAGTPLFRERVLADYRRVFEIDPAIDAGKIAARM
                     DQGVLTLTLPKSEEVKPRKIAVG"
     gene            complement(12041..12511)
                     /locus_tag="PKI07_10600"
     CDS             complement(12041..12511)
                     /locus_tag="PKI07_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOC51539.1"
                     /translation="MNSLIRWSRPELATWPSFGRLTDLRNEIDRLFESPLAEMARSAR
                     LFSGWTPSLDLYEDKDRLVVKVEVPGMKREDIDVSLHEGCLSISGERKGEEKHKEADV
                     YRAERFFGRFQRTVALPAPVAADKVTAQYQDGILTVTLPKTEEAKPRRIDVNVN"
BASE COUNT         2174 a         4475 c         4178 g         2535 t
ORIGIN      
        1 ggtgaatgat tggcggggtt ggtttgggaa gttgcccggg ggctttggtt gcgtggatca
       61 agagccgggg cgattgcggc tgcggggtgg gcggtggttc agcgtttcgg agggggtgtc
      121 ttgccgcgcg gcgaaggctt tgaggttccc gcgcgcgagc gcctgataag acacggcata
      181 gcccggcccc ttgtaaacgc tttgagtcct cttcttccgc ggcaatcacc aacggcggcg
      241 cgggcgttgc gtcaaggcgg tggcaaggcg ggcgattttg gcaatctcgc gccggccggc
      301 ctcgaccgcg gggccgggcg ggggatcggc ccggcccgga tatttaatct ggcccaaatg
      361 cgggcgctcc cgcttaatga gggggcttga ttcaatgaac agctatttca acaccggcga
      421 ctgggtcatt attctcatct atttgctcgg catcattgcg ctgggattgt ggtttggcaa
      481 agaccagcac aacacgcgcg actattttct cggcagcaag aacgtgccct ggtggggcat
      541 tggcatgtcc atcgtggccg ccgaaaccag cgcgttgacg attatcggcg tcccggcgat
      601 ggcctacggc ggcaacatcg ccttcatcca gatgatcatc ggctacgtca tcgcccgcat
      661 catcctggcc atcgtgctgg tgccgcacta tttcaaaggc gagatttact ccccctacca
      721 gttgtttgcc gacaccttcg gccactccgc ccgccagacc gccggcggtt tttttctgat
      781 cagcgaaacg ctggcggccg gggtgcgcgt gtatgtggcc agcatcccgg tcaagctcat
      841 gctcggcgac aaggttctca gcctcggcct gggcgacccc atcttcggcg ccatcttcct
      901 gtttgtggtt ctctcgctgc tctataccta cgtgggcggc gtcaaagccg tcatctggac
      961 cgacgccgtc cagttcgcgc tgttcctcct cggcggggtg ttcgccctgt gctacattcc
     1021 caccctggtc gagggcgggc tgggcgggtt gtttgcgaag gcgaccgcgg cgggcaagct
     1081 gcattggctg aacgccgccc ccgcgcccgg cgcttccttc gcgcagttcc tcctcgacaa
     1141 gccgttcaac atctggatgg gcgtcatcgg cggcacggtc atggtgatgt cctcccacgg
     1201 cgccgagcaa ctcatcgtgc agcgcgtcct ggcctgcaag aacgtcgccg acggccgcaa
     1261 ggccctcatc ctcagcgcgg tggttatctt ccccctgttt ctgatcttcc tgctggtggg
     1321 cgccatgctg tgggtgttct accaagaccg ccccttccag attccgctgc ccgagaccag
     1381 ccccggctcg ggcatcaccg ccaatgattt catctttccc atcttcatga tgacggaagt
     1441 gccgcacctg ctgaagggct ttctgatcgt ggccatcctg tccgccgcca tgtgttcggt
     1501 cagctccgcg ctcacctcgc tggcctccgt ttcgacgatg gacttcgtga agcacgtgtt
     1561 tcccgggcgc agcgaggcct tctttctccg tttcagcaag atttccaccg tggtctgggc
     1621 cgccgccctt attctggtgg cctacctgag ccggcaggtt ccgtttgtgc tgaacgccgc
     1681 tttcgccctg cgcggcctca ccagcggcgc cctgctgggg gggctggccc tggccatctt
     1741 ctggcggaaa ggccgcgcgc tgcccatcat tgccgggatg ctggtctcgc tcggctgcat
     1801 gaccgccatt caactgctgc ccaaattcgc cctgacccgc gactggtgga tgaagaccat
     1861 cggcaccgaa atcttctggc cctggtacac cctcattggc gtggtcatca ccctcctcac
     1921 cgccggactc gtgcgcaggc tcctcccgcc tcccgcccgg taggcgggct tttcggcgcc
     1981 ggccaaccgc gccccgtccc gccccgggcg cggccgcggg ggaccgggaa agagccgggg
     2041 cggcgcgcgt tccgcgcctt aaagactcac gttcaggccg tggtcgcgca ggtattgctt
     2101 gagctccgga atgcgaatga ggccgaagtg aaacaccgaa gcggccagga gcaccgtcgc
     2161 ccccgccgcc gccgcttccc ggaagtgttc catcgttccg gcgccgccgg aagcgacgac
     2221 ttccgccggg gtggcggccg cgatgccgcg gatcagcggc aggtcaaagc cggtgcgggc
     2281 gccgtcggcg gccttgctgg tcggcaggat gcattgggcg ccgtagtccg cgacccgccg
     2341 cgcccagtcc agcgcgtccg cgccggtggc ggtgcggccg ccgtccacga acacttcgta
     2401 gcccgagggc agcgccgggt tgtggtccac gtcaatcgcc acgatgactt tttcggcgcc
     2461 gagctcgcgg atcatctcgg cgatgaccgg ggggttgcgg aaggcggcgc tgctggtgga
     2521 cactttatgg gcgccggcgt cgaggacggc gcgggccgag gcgacgtcgc ggatgccgcc
     2581 gcccacggtg aacgggacgg tcgccaccgc cgccacgcgt ttcaccacct ccagcagggt
     2641 gccgcggtgt tcgacggtgg cggtgatgtc cagcatggcc agttcatcgg cgccggccgc
     2701 gcaataggcc cgcgcgcagg ccgccgggtc gccggcgtcc tggatgtcca cgaaatgcac
     2761 gcccttgacc acgcgtccgt tttgcatgtc gaggcagggc atgatgcgaa ttttcggttg
     2821 cataaatagg ttttcggcgc ggcgcggcgt ggcggcgctc agaggtattc caccgtggcg
     2881 gggggtttgg gcgtcaggtc atagacgcac cggttgacgc cggggatggc cgtgatgcgc
     2941 cggctcaagc ggcgcagcgt tggccacggc aattcgcgca cggtggcctg gcgcgcgtcc
     3001 gtgctgtcaa tgcagcgcac cacgatgatc tgcccaaact cgcgccggcc gtggcggatg
     3061 ccggtggcgc ggtcgttgag cagcacggcc aggtactgga acgcgccggt gcggcggagt
     3121 tccgcctcga cgatggcggt ggcctggcgg acgatgtcca gccgggccgg ggtgacttcg
     3181 cccacgatgc gggtggccag cgcgggcccg gggaacggga tgcgccgggt gacgctcgcc
     3241 ggcagcccca gcaacgccgc cacgcggcgc acgccgtcct tgcgcagcgt ggccaggggt
     3301 tccagcacct tgtagccata agcctgctgc gggttgatgc cgagctgcgc caggatatta
     3361 tgctgccgct tgatgcccgc cacggtttct tcgatgtccg tcaggatcgt cccgtgcagg
     3421 aggaaggtcg cccgcgcgcg cttgaccgct ttgccgaaga cgccgcggta gaatgtgtcg
     3481 gtgatggcct gccgtttttc ttccgggtcg gtcaatcctt tcagggcccg caggaactgg
     3541 cggcgggcat ccaccaattc gaccgggatg cccaggtcct ggaaggtttt gaccacgagt
     3601 tgcggttcgc cctcgcgcat gagggcgtta tccacgaaga tggtcttcaa ctgccggccc
     3661 agggcccggt gcgccagcac ggttaccacc gcgctgtcca ccccgccgct cagcgcgttg
     3721 atggcggtcc cgcggccgac ctcgcgccgg agggtggcga tctgattcgc aataaaggtt
     3781 cgatcattca taggcattcg ggcctgttcg cactatgtag aacgattgcg cggggaaagc
     3841 cagcggaatt tttggcgccg ggaaccggac gggaaaagcc ggcggcggtc agtccggcgg
     3901 cggcggcgcc gtcagctcga gctttaacgc ccccagggag aggtgaagtt cccggagaaa
     3961 agcggccagc gagccgatca gggccgccat gcaggcaata aacagcaggc tgatcagcca
     4021 ggccgtcccc gccggccgca gcgccgccag gaacagcgcg ataatcaaga ccgacgccag
     4081 cagcacgctc accccggcca gcgtgatgga caaccgcagc aaccgcgccc ggcggtacag
     4141 gatcgccacc tgcccttcga gccgcgggcg ctcggggccc gcggcggcgc gcagctcccg
     4201 cagcagttgg cgcgaacggt caatggcccg cccgtagcgg ttcgtcagcg tcagcagcag
     4261 caggcccacg cccgaaatca ggatcaccgg gccaatggcg atttgcagca cgggaatcag
     4321 ttcttgcacc ggcgtcgaaa ccataactcc tccagcctaa cgccgccccg ccgccgggca
     4381 atcttattcc cggccctggc agagggcgcc tcctggtttt tgcggcgggc agtttccgcg
     4441 gcgggtcagg tccacaactg gcgatcgagc gaacggtatt ggatggcttc ggagatgtgc
     4501 tcgctggcga ggttgtccgc gccggccagg tcggcgatgg tgcgggcgac cctcaggatg
     4561 cggtcgtagg cgcgcgcgct gaggttcaga tcgctcatgg caaacttgag cagttccatc
     4621 gtcggctcgt ccagggcgca ataggttttc agctcgcggc tgcccaggcg ggcattgcag
     4681 gtgatcttcg gtttatcccg caggcgcgtc tgctggcgct ggcgggcggc cagcacccgc
     4741 gcccgaatct gcgcggaggt ttcgcccggg agcgggctgg tgatctcgcg gaatttgacg
     4801 gcgggcactt cgacgtgcag gtctatgcgg tcgagcagcg gcccggaaat gcggccgagg
     4861 tagttctgga tttcgcgcgg cgaactgcgg gattcggcgg gcattttgcc gtccggcgtg
     4921 gggttcatgg ccgccaccag catgaactgg gagggaaagg tcatcgtgcc ggcggcgcgg
     4981 gagatggtca cgtggccctc ttcgagcggc tggcgcaggg tttcgagcac ggtgcgtttg
     5041 aactcgggca gctcatcgag aaacagcacg ccgtggtgcg cgagggagat ttccccgggc
     5101 gtggggttga cgtttccgcc cagcaggccg gcatcgctgg cggtgtggtg cggcgcgcgg
     5161 aaggggcggc gggtgaccag cgcctggccg ggcgcgagca ggccggcgat gctgtgaatc
     5221 ttggtggtct cgagcgcttc ctccagggtg agcggcggca ggatggtggg cagccgcttg
     5281 gccagcatgg atttgccggt gcccggcggc ccgatgagca ggacgttatg gccgccggcg
     5341 gcggcgatct cgagggcgcg cttgacggat tcctggccct tgacctccgc gaagtccagc
     5401 tcgccgtccg gcgcctggtc gaatatccgc gccacatcca cttgcgtggg ggaaatcggg
     5461 agcgagcctt cgagaaaact caccgcctcg cgcaggttgc ggaccgggat gacttgcagg
     5521 cccgccacca cggccgcctc ggcggcgttg tcgggcggaa ccaggaggcc gcgtttgccc
     5581 tgtgcccgcg cgcgcaaggc gatgggcagg acgcccttga ccgggcgcac ggcgccggtc
     5641 agggccagtt cgccgaccag ggcgaagtgg tccaattggc cgctttccat ttgctcgctg
     5701 gcggccacca tgccaaccgc gatggggagg tcaaagctcg gcccttcttt cttcacgtcc
     5761 gccggcgcca ggttgatggt ggtgcgccca aaggtgaagc tgaacccgga attggccagg
     5821 gcagttgtga cgcggtcgcg cgattctttc accgccgcat cgggcagccc cacaatcaca
     5881 ataagggtgt cgccgtagcc ggcattgact tcgacttcga ccggatacgc ttcgatgccg
     5941 ctgaccgcgg cggagcagac cttggccaac atgggccttg ttatgggaca gcagggggaa
     6001 aagcgcaatg cggaaatgcg gcgcggacgc gggcgccgcc gcccggttct cccccggagc
     6061 gcccggaaac gccggggcgc cattggccgc caaatacgcg caaatattcc ttgcctttcc
     6121 gggcggctcc agataggatt cccccatgca cgatgcgcct aaaaaccacg tagtgaaggc
     6181 cctccgcatc gggtcccggc tgcgctcatg ctggcaagcc gtggcggcgc cgggcttgtg
     6241 gctgctcatg tcggcccttc ccgctttcgc ccaaccctac gccatagact ggttcacgct
     6301 ggacggcggc ggcggcacga gcaccggcgg ggtgtatgcc gtgcgcggca ccattggcca
     6361 gcccgacgcg ggccccacca gcctgaccgg cgggccttat tcgctctccg gcggtttttg
     6421 gacgatttcc gcggtgcaaa cccccggcgc gccgctgtta tccatcgtgc gcacctcgac
     6481 gaatacggtc gccgttttct ggccctcgcc ctccaccggc tgggtgctgc agcagaacgc
     6541 gcaaaacctc ggcactccga attggagcaa tgttcccggc gcgccgattg acaacggcac
     6601 caaccggact gtgatcgtca atccacccgt gggtcaccgc ttttatcgcc tgtttaaacc
     6661 gtaagctttc cggcccgatt cagagaaccc cactttttaa tccgtaatca ctatgcaagc
     6721 taaaaccttc atccgctgcg ccagtctggc gttggtcttc ttaggcaccc tcggtctgtc
     6781 cggcgcgcca gcctgggccc agggaacggc gtttacctac cagggccatt tggccagtgg
     6841 ctccggcggc gctaatggga tttacgatct gcagtttacg atttacaacg ccagcagcgg
     6901 ggcgggggtg gttgcggggc ccttgaccgc ctccgccgtg gccgtgagca acggcttgtt
     6961 cacggtcgtg ctggatccgg gcgtcggggt gttcacaggc gcggagcgct ggctgggaat
     7021 agccgtgcgc accaacggcg ccggcgcctt cacgacgctc gcgccccggc agcgaatcac
     7081 cgccgcgccc tatgccgtca tgtcgagcaa catcgccggc accgtcgccc ccgcctccct
     7141 gccgcccggc ggcagttggg cgctgagcac cacgctgacg ctggacgccg gcacgctggc
     7201 cattgacccg gtcaacaacc gggtgggcat cggcacgtcc ttcccggcct acccgctgca
     7261 cctccaggcc gggcagagcg tggcccggct ggacagcacc gccaatacgt tcgggtcggt
     7321 aatcgaattg cgcaatacca ccgcttggcc cacctatctt ggcgcgatca atttcaacaa
     7381 cgccgccgga acgtatcccg gccagatcgg ctacctggcc accaatgtta tggtctttcg
     7441 caccggcggc gcggagcgaa tgcgcctgaa cgatagcggc cgcctggata tcaccgggac
     7501 ctattcctcc gtcatcgtga acaacataaa ccactactcg ggcctcccat accccacggg
     7561 catttctgcc actagcgact cggtcttcgg taccgcaatc cgcgcctcca gcgaccatgg
     7621 caccgccctc tccgcgacca gctccgacgg tctcgcggga ttgttctctg gcgcggtgca
     7681 aatcaattat gcttccccct tcagcaaacc gcaactgcat ctgaaagacc cctcgacgaa
     7741 cggcttttcc cggttgcgca tgcagacggg gtcgcggccc ttttgggata tcgcggtggg
     7801 cggcgataac tccctgcgct tcttcgccga cggcaacggc gatgtggtga cgctccaaac
     7861 caacgggcag ctctttgtca aagtgctcac catcaccggc ggggcggaca tcgtcgaacc
     7921 gttccagatg tcagaaccgg aactgtcccc gggcgcggtg gttgtcattg atgaggcaaa
     7981 tcccggcaaa ctgaagctca gttccgcgcc ttacgaccgg cgcgtggcgg gcgtcatcag
     8041 cggcgcgggc ggggttcgcc cgggactgtg cctccggcag gaaggccggg tggaggggga
     8101 tcagccggtg gccctgaccg gccgggttta cgtgcaggcc gatacggtca atggccccat
     8161 tcagccgggc gatttgctga cgacctcgct cacgcccggc cgcgcgatga aagtgactga
     8221 ccacgcccgc gcgcaaggcg ccatcctggg caaggccatg accggcctgg ccgcgggcga
     8281 ggggttggtg ctcgtgttgg tgagtttgca gtaaactgaa ccggcttatg acccggctaa
     8341 tcctgcaaag cgcgctggct ggattgcttt ggctggccgc cgcggcggcc agcggccaga
     8401 cgatcaacct gcgcatgtcg gtaaaggtga tcgtccaccc cggcaccggc gcgtggcccc
     8461 ccggcatcac ggcgcaagtc tttaccaacg cggtcgccgc ggccaacgaa tggatggcct
     8521 cttactgtcg cggctaccgg tatcaactca cggaattaca ggccattggc gggccaagcc
     8581 agggcggcac gaatggcccg agccgctggt atggcttgga attccgcggc gacccgctcc
     8641 gccggcagtt ctttaacctc gccaccaacg atacccggta tctgctgcgg ttcgaccaga
     8701 tcaacatcta tgtggcctcg gggtttgcca accccggcga cagcgggggg gccatgccga
     8761 ttccgccggg ggagacgaac tatatcggcg gccagatcta tgccgacgac ggggcctggt
     8821 ggattgtgca tgagctgggc cacttttttg gcctgccgca tacctttgcc acgcaaccca
     8881 aggacagttg cacgcccggc gatgatggct ttacggatac gctcctggac agcacctgct
     8941 ggtcgaacca gaaccaggtg gcacagttcc acttcggcgc ggactatgac gagcttaaca
     9001 acaaccagaa ggaccaggtg gatacggttt tctttaacgt catgtcttac catgattaca
     9061 cgaacaagaa caccacggaa aaccggctga cggagcagca gctcgaccgg attaccgacc
     9121 acgcgaacgg cgaccgccat gcctttgttg gcggatatac ccgctttgtc agccccagcg
     9181 gcaatgacag caactccggc ctcgtcaaca acgcgcccaa acgcaccgcg ctcaacgccg
     9241 tcaacgcctc cgcgtccggc ggcggcgata tcgtgctgct gcgccccggc aattataacg
     9301 agcggaccaa tttcaaccgg cgggtgaccc tgcgcgccac ccgcgccggc tgggccacca
     9361 tcggcaagcc ctgaaccgcc tccccggacg ccccgctttc gttcttcaag tgggcggggc
     9421 cttatgccac attccggccc gatggccgcc tacgcgctga cgatttttgc cggggccttt
     9481 ctgttgttcc aggtgcagcc gctgatgggc aagtacatct tgccgtggtt cggcggcggt
     9541 cccggcgtct ggacgacctg cctgctgttc tttcagacgc tgctggtcgg cggttacgcc
     9601 tacgcgcacg gaagcgcgcg ctggctgaaa ccgcgcgcgc aggccatcgt gcacctggcg
     9661 ttgctggcgg gcgccctggc gctgctgccc attacccccg gcgatgcctg gaaaccgtcg
     9721 ggcgccggca accccacctg gcagattctg cgattgctgg ccgcctgcct cggcttgccg
     9781 tacctggcgc tcgcggcgac cagcccgctg cttcagcatt ggttcagccg cacccatccc
     9841 ggcacgacgc cttaccggct ctatgccctg tccaatttcg gctcgttgct cgccctgctg
     9901 agctacccgt tcttttttga accccattgc acccgcctca cacaagcccg gctctgggcg
     9961 tggggcctgg tggcgtacgc catcggctgc gggttctgcg cggtgaaaca ggccaaagcg
    10021 gaaaaccggg gaggagcggc cgaagccccg cccgcgcccc tcgctcctgg cccgcccccc
    10081 tcggccgcgc cccgctccat cacccggcgc ctgctgtggc tcctgctccc cgcctgcgcc
    10141 tcggtgctgc tgctggccgt gaccaataaa ctctgccagg acgtggcgtc catccccttc
    10201 ctgtggatcc tgccgctggc gctctacctg ctgagcttca tcatttgctt cgacagcccg
    10261 cgctggtatc ggcgcgttcc ttacgccctg gcgctggtcg gggcgctggc cgccctgggc
    10321 tgggcgctgt ttaacgccag cgacctgccg ctgcgccagc aactggtgat ttatggctgc
    10381 gggcttttcc tctgctgcat ggtctgccac ggcgaactct accggctcaa gccgccgccg
    10441 gagcagttga ccggctttta cctggcgatc gcgctgggca gcgcgctggg cggcctcttc
    10501 gtcgccgtcg gcgcgccgct cctgttcacg gactactatg agctgcactg gggattgctg
    10561 ctgtgcggcc tcttgtttgt cttcgtctgc ctggcggagg ggcgcctgtt tccgccccct
    10621 cccgccctgg accgcaacgc ccccgggccg ggccggcgct ggctggcccg gttggccggg
    10681 ccgtggtttc tccgcggaaa cctcccccgc ttccgctacg ggcgcgagct gtccggcgcc
    10741 gggttgctcc tgggcgtgat cgggctggga gcggttctct ggtgggaaac ccggactgcc
    10801 gccgcccagc gcgtcttcca gtgccgcaat ttctacggca cgctgacggt ttacgaatat
    10861 gcgccggaga acccggaatc ccactgcttt ctcctccagc acgggcgcat cgcccacggc
    10921 gtgcaatttg ccagctccga acgcgcggcc acacccgtta gctactacgg ccccggcagc
    10981 ggcatctggc tgggaatgaa cgcgctgccg ccgggccccc gccgcatcgg cgtggcgggg
    11041 ttgggagcgg gctccatggc cgtcttcgcg cgcgcggggg attacctgcg gttctatgaa
    11101 atcaatccgc aggtggagca ggttgccgcc gcgtggttca cctatttgac caattgcccg
    11161 gcgacggtgg aaatcgccac cggcgatgcg cgcctgtcat tggagcacga accgccgcag
    11221 aacttcgacc tgctggtgct cgatgccttc aacagcgatg ccgttcccgt tcatctgctg
    11281 acccgggagg ccttcgccgt gtaccggcgg cacctgcggc ccggcggggt gatcgccatc
    11341 aacatctcca atcatcacct caacctcaaa cccgtcgtcg ccaacctcgc gcgccacttc
    11401 cactgccaat ggatctccat agactacgag gaaaccgacg aggaatggtg gctctacagc
    11461 tcgacctggg ccctgatgtc agacgacggg aaagtcatca actctcccgc cgtccgtaat
    11521 gccgccagtt ctccctgcga ccccgtcacc cccagcccca tcccgctctg gaccgacgac
    11581 ttcgtcagcc tcttccagat cctctactag cccaccgcaa tcttgcgcgg cttgacttct
    11641 tccgacttgg gcaaagtcag ggtcaacacc ccctggtcca tcctggccgc gatcttgccg
    11701 gcgtcaatgg ccggatcaat ctcgaatacg cgccggtaat ccgccagcac ccgctcccgg
    11761 aagagcggcg tgcccgccac cgtctcgttc tgccggcggc ccaccagcgt aatctcattg
    11821 tcctccagcg tgatctccag gccgtccttg tggacgcccg gcatttccgc ctcgacgacg
    11881 tagccgtctt tggtttcgta gatattcact tccggcgtca cataacccgg ctcgtgccgc
    11941 ggttccgcct tggctcgttg ttccgtctgc atcgtagttt tcatctttcg catcctttcc
    12001 caataaacct cccggcgggc gcacccgccg ggaagggagt tcagtttacg ttcacgtcaa
    12061 tccgccgcgg cttggcttcc tccgtcttgg gcagcgtgac ggtgagaatc ccgtcctggt
    12121 actgcgccgt gaccttgtcc gccgcgaccg gggccgggag cgccaccgtt cgctggaatc
    12181 gcccaaagaa gcgttccgca cgatacacat cggcctcttt atgcttctct tcgcccttgc
    12241 gctcccccga aatgctcagg cacccctcat gcagcgagac atcaatgtct tcgcgcttca
    12301 tgcccggcac ttcgaccttt accaccaggc gatccttatc ctcgtaaagg tccaaacttg
    12361 gcgtccagcc gctaaacaag cgcgccgaac gagccatctc ggccaacggg gactcgaaca
    12421 agcggtctat ctcgttccgc aaatccgtca atcgcccaaa acttggccat gttgccaact
    12481 ccggtctgct ccatcgtatc aaactattca tagtattatt cctttctttt ggttttacta
    12541 tctgccaatc attaagcgtt ttatatgcca atagtcgcaa taattcataa ctactgcaat
    12601 tacaacagaa tacatcattt tactatggat aaaaagacca taaaagtgtg ccaaaatgtc
    12661 ccacatctgt gacggattgc gccgcaaagg tgtgccattt tgtctctact aggcaaatcg
    12721 tgcgtcctat accgaaaacg tgcgctaagt ccgctcctgt ttcctcccac aggtgcctga
    12781 agaccgccat tagcccatcc agcaatttct ttccaagctg cttttcgccc aatacaccgc
    12841 aaacctaagc caagacgatt gggttgggca gttcaaccgc ccgcccgccc tccatcagag
    12901 ccagatcagg cctgaccgca aaaactgccg ccctggcgaa cccttggccc agcaaatcca
    12961 agagcatggc ctctgaatcc cgagcccttc accggtaaaa ccaatgattt gggcaaaaat
    13021 agggccaaaa caaaggcaaa atcggaatcg cgccggcttc gccgcacctt ggacgccccc
    13081 tggttttacc ctgccaatag gccgtgtaca aggggtgtac aagggatgcc catcgggtgt
    13141 accagctaaa atctgatgga ctcccggtga gcatcccttg tacacccctt gtgcacggcc
    13201 tgatgtcagt gggaatccaa ggtccctgcc ggataatggc taccggtgtc cggaaaggcc
    13261 gaaacaagga gagaggatag ggggataatt tctaaatccc ataaaaacag gtagtgtcct
    13321 aaaagttctg caaaaagcag cgggtcatgg taagccactg gg
//
LOCUS       DAOPQG010000096        13360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5383_length_13360_cov_12.129726, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000096 DAOPQG010000000
VERSION     DAOPQG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 13360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13360
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5383_length_13360_cov_12.129726"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..2042
                     /locus_tag="PKI07_10605"
     CDS             363..2042
                     /locus_tag="PKI07_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51540.1"
                     /translation="MKSFIQTKPGVLLGGAVLALALVMAAPSAHANVYASNIKINGEL
                     LAAQAEQGQNVNISYILNEPASDGVTINILSNAVVIRTLNVAGGGAGALRGANTVVWD
                     GKDAGGNFVGLGADYSVSITAASTGYASWTTTTDDSEPFLVWEGRGIAVNQNPKSPYY
                     GRIIVCNSYNGPNLSVPGYNVGMLKLNADGSYAEEGGHSTGGHTWGNDYYSPWHVRIS
                     ADDYVYINDWSASGEIYRWDMTLSAASKLHVLSSDNWAPGCNMSGPEIVGTGTNTIIY
                     MADIAYPNSQGVVKYTVTEDGTCDQYDIGTPVIPISYPLSLYPYDVAVDKNGNFYIVQ
                     YRSNVTDPSPRVMCYDSDYNPVWNTADIYGGLGQADDTWAYANAVAVDPTGTYVAVAF
                     RGNAGGWGCTRIFDAATGAIVTNLDYGLDINGNGTEHSDRACGWDAVGNVYYVDNWYG
                     AWRAVSPPGANSSTTEPPVTLEIVAAPPPAPVITSCSLALGTFTLNFTGGVAEVPGDF
                     EILSSATVDGTYSYAAGQNITGGSGVFQATIPAPDQAQFYRVHRVEAAQAR"
     gene            2193..3011
                     /locus_tag="PKI07_10610"
     CDS             2193..3011
                     /locus_tag="PKI07_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51541.1"
                     /translation="MHARSLNPAPARRGGARAFTLIELLVVIAIIAILAALLLPALAR
                     AKDKAQAAGCLNNLKQLQVGWVMYSDDNVNVLIPNAPSGFPPWQTWCSGTTVGWGNVN
                     ANTNRTPYLQSLMAPYMVNQIGVYRCPGDNVPSFNGRRLRSYSMNCHMGSIYSGPLTQ
                     SYNPGWKVYQKITDITQPAPADAFVFIGEHAGSINDGYLQVRSNAPEFPDVPGSYHGK
                     SEGVSYADGHAALHKWWTAALAVPVAYGVAYKSVKVAADNADWIWLRDHASVKE"
     gene            complement(3001..4449)
                     /gene="gltA"
                     /locus_tag="PKI07_10615"
     CDS             complement(3001..4449)
                     /gene="gltA"
                     /locus_tag="PKI07_10615"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOC51542.1"
                     /translation="MSPKLSTRQRLQIERQPMPAQAPEVRRANFAEVNLGLPERIALL
                     EAERCLLCKDPKCVAGCPVGINIPRFIQLLTEGKLPEAAHCLLNDNPLPAVTGRVCPQ
                     ETQCELECIRGYKGLPVAIGHLERFLGDWARGHAQRPALEAAPATGKQVGIVGAGPGG
                     LTAAGELARLGHQVTVFEALHKPGGVLVYGIPEFRLPKQIVREEVARLERAGVKILCN
                     TVIGRTYTLAELRARFDAVFIANGAGLPVFMNIPGENLKGVYSANEYLTRANLMGARR
                     SARAGTPILTGRRVATIGGGNVALDAVRTAKRLGAESATIVYRRTRKEMPARAEEVLH
                     AEEEGIQFEFLAAPVAIQGDARNWVTGLQCVRMELGEPDASGRARPRAIPGSEFLVPC
                     EVVIVAIGTRANPLLAGTCPELKLDSRGGIAVDEQGMTNLPGVFAGGDIVRGAATVIL
                     AMGDGKRAARAIHEYLRRPVSPARAPAERITP"
     gene            complement(4584..5423)
                     /locus_tag="PKI07_10620"
     CDS             complement(4584..5423)
                     /locus_tag="PKI07_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOC51543.1"
                     /translation="MHKIIAKASLSPQIIRLDVAAVRIARIRQPGQFVIVHRGGDAER
                     IPLTIADADAEAGTITLVIQVAGQSTCELAALNPGEAIANIAGPLGHPTELIAAGRAL
                     CIGGGVGVAVVHPIAQGLKRRGVTVSSIIGGRTREAIIFEPELRRLGEVIVCTDDGSY
                     GRRGFVTDAARDALAAGGIDIVYAVGPVPMMRAVANLTRPLGVRTIVSLNPIMVDGTG
                     MCGGCRVSVGGQTRFACVDGPEFDGHAVDFEQLMDRLTTYRDFEQRALAACQEGDCKL
                     GRR"
     gene            complement(5554..6531)
                     /locus_tag="PKI07_10625"
     CDS             complement(5554..6531)
                     /locus_tag="PKI07_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51544.1"
                     /translation="MMSYCKFSFPVGGRRVGRAALGLGVILGILALTPGLRASENVPH
                     RPFAQWANVPERGQFVIGLVYEESEAYHIWAGGRQHDITTRSHGEKYGIDINQGYLTA
                     QYGITERWAADLSVGATTVGWRKFAPDEHADSTTGLMDWSFGVRYQIFNEAAPRQSPW
                     VPTLTFRAGAVLPGSYDKNFPFAPGQRSAAIEPELLLRKHFGWPGLGVYGDALYRWNR
                     TTGNDQYLIAVGLFQQIKGWELDVGYRRLHSVAGDNIRYDPAQPAALDYPRDVREISD
                     ALEAGFSYTTAKRHWRYAFHLRSVLDGNNSDSKLWVGGSFEIPLGPRRR"
     gene            6695..8044
                     /locus_tag="PKI07_10630"
     CDS             6695..8044
                     /locus_tag="PKI07_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51545.1"
                     /translation="MNANPPDRPARSSRRAFLQTSSTALAGAALSGVLARPGYAAENN
                     TLKLALVGCGGRGSGAAAQALSTRGPTRLWAVADVFAHRVQTCLAGLKSSHAAQVDVP
                     PERQFTGLEGFRQAMDTLGKGDVVLLATPPAFRPRHLEYAVQKGLHVFMEKSFAVDAP
                     GIHRVLRAGAEATAKGLKIVGGLMSRHYRPLEEAIQKIHDGLIGDLVTCWAYRMHGPV
                     ALNPRAAGLTELAHQIANYSCFTWLNGSFLEDWLIHNLDVCCWAKNAWPVSVQGMGGR
                     QVRQEPDQLFDHYTAEYSFADGTRLLAQGRHMTNCWDFFGNLLHGTRGSGILGEGQPR
                     PRLYRDWRPAAENLAWSYTGPACDPYQAEHDLLFSAIRNDLPCNETERCAKACFTAIL
                     GRMACESGKRVTWDEAFASTTELAPGLDHYTLDSTPPVVPDAAGRYPLALPGQTPPF"
     gene            8250..10061
                     /gene="rpoD"
                     /locus_tag="PKI07_10635"
     CDS             8250..10061
                     /gene="rpoD"
                     /locus_tag="PKI07_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOC51546.1"
                     /translation="MRKSKPEKKRRPVSKKAPSRASRRAPAPTAPPARAPRRRSRRPA
                     AATPAAPAPAPASPPPALDLAQKVKELLRLAKEQGYLTYDDLNDALPDELVTPADLDQ
                     VLLKLRGLDVEIIDAAEVDHGRRPDGEEEGEEKRYDLLDDPVRLYLRQMGKVPLLTRE
                     QEVAICQRIEAAEAEARRILQSFGLAPKEYLALAEKLLATPPRERFDRVVADQSPLHR
                     KRYLHSLRRLVKRVRALDFQADARFAAWQRATRKADRARLHYQLQRFHRKLDALYPRF
                     GFKRKVVDDMALVVENLHLKLQASLQALAAPPARQNPAARRAFLRAEHDKIRMWERFA
                     RMPHADFLTARAQLNDARARAQEAKREMVEANLRLVISIAKKYTNRGQSFLDLIQEGN
                     VGLMKGVEKFEYRRGYKFSTYATWWIRQAITRCIADQARTIRIPVHMIEVINKLWRTQ
                     KQLTQELGREATTEELADSMEMPLERVRVVLKLAQQPVSMQTPVGEADDASFGDLLED
                     KSAENPGDVTSFHLLRGRLSEVLGSLSERERRILELRYGLHDGFPRTLEEVGQQYEVT
                     RERIRQIEAKALRKLRHPVRKSKLEGFLESSPAPRLA"
     gene            10118..11398
                     /gene="tyrS"
                     /locus_tag="PKI07_10640"
     CDS             10118..11398
                     /gene="tyrS"
                     /locus_tag="PKI07_10640"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOC51547.1"
                     /translation="MNILDELQWRGLLADCTDAPGLARRLAAGPLTVYCGFDPTADSL
                     HVGHLVPLLALRRFQRFGHHPLPLAGGATGSIGDPSGKSQERQLLTRETLAANIAQVK
                     RQLQRLLDFDSPPNPARLLDNATWTAPLSYLDFLRDVGKHFSVNQMVAKESVRARMQD
                     RDAGISYTEFSYMLLQAFDFYVLCRDYQCELQIGGRDQWGNITAGIDFTRKKLARTVF
                     GLTLPLITNADGSKFGKTVAGAVWLDPARTSVYRFYQFWIRADDRDVIRYLKYFTFLS
                     RDEIAALEQQHTANPGARAAHQALARSVTELIHGPDATAEAIRASQILFGGDLAGISA
                     AVFNEIVGEVPSRPLNRRELEGPGKPLVELFVLSGLAPSKGQARKDIEAGGAYLNNRR
                     ATNPLHPVTTADLLFNRHLLLRKGKRNYVVLSAA"
     gene            11407..12678
                     /locus_tag="PKI07_10645"
     CDS             11407..12678
                     /locus_tag="PKI07_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOC51548.1"
                     /translation="MILYNLFPLLAGPLHRWEPHLDRARALGFNWLFLNPIQLPGASG
                     SLYSIADYFRFNPRLLNPADPAPPETQIQRLAARARERGLRLMIDLVINHCAIDSPLI
                     REHPQWFVRQPDGRPAHPSCQHHDQTVVWTDLAQFDHQHPRDPEGFYRYCRRVVDALL
                     ALGFDGFRCDAAYQIPRPFWQRLIHDTRQRHPHACFAAETLGCSPAQTLETARAGFDY
                     VFNSSKWWDFHGCWLLEQYNLIREITRSISFPESHDTPRLAAELNGNPHALKQRYLFA
                     ALFAAGVMMPIGFEFGFRKPLHVVNSQPADWEPVHVDLSDFITRVNHLKLQHPVFREE
                     SPASILPTDNPNILLLWKASVQHPQEALLILNKDPHHHQDFHTDHFRRFIQSGAPLRD
                     VSPEYPLDYLPEPFHYALRPGQGIVLVTTRS"
     gene            complement(12686..13303)
                     /locus_tag="PKI07_10650"
     CDS             complement(12686..13303)
                     /locus_tag="PKI07_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOC51549.1"
                     /translation="MLTSLVVGSFLGISCGVMPGPLLALVLAQTLRHGAREGCKVALS
                     PLLADVPVIGVTMALAARLTEFDRLLGWVSVAGGVFLLYLAGESWVAARRGVVCAEAS
                     PRSWSKGLGTNLLSPHPWLFWLTVGMASLTKALAGGWLAAGAFLLGFYGWLVGIKVAV
                     ALLAARSRDWMTGRVYRVGLRCLAVLLGGFALLLLREGGRYLGWV"
BASE COUNT         2389 a         5105 c         3855 g         2011 t
ORIGIN      
        1 ggattttagc tggtacaccc gatgagcatc ccttgtacac cccttgtaca cggcgtagag
       61 atagggggtg ttttggcagc tatgtcgctg attttactga agaaaggatt tgccggaact
      121 atggcctggg cgatcgggcc tgcccaggct ccgagggggc gaaggcgaac ggttgaacta
      181 ttcaactgaa acgttccgat ttaagccgga ctgttggctt cgcaaaagcc cggacgcgcc
      241 caaggcgaaa aaatgcgcga agttagactt gccttcggca ggggggccgt gctaaagttg
      301 gcacatggca tgggctcgta gcccatacgt tagatcaacg caacgcaaat aaaaacaaga
      361 ctatgaaatc cttcattcaa acaaagccgg gcgtcctcct gggcggcgcg gtactggcgc
      421 tggcgctcgt catggcggct ccgtccgctc acgcgaacgt gtatgcgagc aatatcaaga
      481 tcaacggcga gcttttggcc gcccaggccg agcagggcca aaatgtcaac atcagctata
      541 tccttaacga acccgcctcc gatggggtga cgattaacat cctgtccaac gccgtggtga
      601 ttcgcaccct caacgtggcg ggcggcgggg ccggcgcctt gcgcggggcc aacaccgtgg
      661 tctgggacgg caaagacgcg ggcggcaatt tcgtgggttt gggtgctgat tattcagtca
      721 gcatcaccgc cgcttcgacc ggttatgcga gctggaccac caccacggat gacagcgaac
      781 cctttctcgt atgggaaggc cgcggcatcg ccgtgaacca gaatcccaag agcccctact
      841 atgggcggat tattgtctgc aattcctaca atggtccaaa tctatccgtc cccggttata
      901 atgtgggcat gctcaagctc aatgccgacg gcagctacgc cgaagaaggc ggccacagca
      961 ccggcggcca tacctggggc aatgattatt acagcccctg gcatgtccgg atttccgccg
     1021 atgactatgt ctatatcaac gactggtccg ctagcgggga aatctatcgt tgggatatga
     1081 cgctctcagc cgcctccaag ctgcatgttt tgagttcgga caactgggcg cccggctgca
     1141 atatgagcgg gccggaaatc gtgggcaccg gcaccaacac catcatctac atggcggata
     1201 ttgcctatcc gaattcacag ggcgtcgtta aatacaccgt gaccgaggat ggcacctgcg
     1261 accaatacga catcggaaca cccgtcattc caatcagcta cccgctcagc ctctatccgt
     1321 atgatgtggc ggtggacaag aacgggaatt tctacatcgt ccagtaccgt tccaacgtca
     1381 ccgatccctc gccgcgcgtg atgtgctatg attcggacta caacccggta tggaacacgg
     1441 cggatatcta tggcgggctg ggccaggccg acgacacttg ggcctatgcc aacgccgtgg
     1501 cggtggaccc gaccgggacc tacgtggcgg tggcgttccg cggcaacgcc ggagggtggg
     1561 gctgcacccg gatcttcgat gcggccacgg gagccatcgt cactaacctc gattacggcc
     1621 tggacatcaa cggcaacggc accgagcatt ccgaccgggc ctgcggctgg gatgcggtcg
     1681 gcaatgtcta ttacgtggac aactggtatg gcgcctggcg cgcggtttcg cctccgggcg
     1741 cgaactcgtc caccacggaa cctccggtga cgctggaaat cgtggcggcc ccgccgccgg
     1801 ctccggttat cacgagctgc tcgctggcgc tgggaacgtt caccctgaat ttcacgggcg
     1861 gcgtggccga agtgcccggg gatttcgaaa tccttagcag cgccaccgtg gacggcacgt
     1921 acagttatgc cgccggccag aacatcacgg gcggcagcgg cgtgttccag gcgaccatcc
     1981 cggcgccgga ccaggcgcag ttctaccggg ttcaccgcgt ggaagcggcg caggcgcgct
     2041 aggcgggggc ctggcggaac gccggcgggg gcgcaagccc cagccaatat gcccgggcat
     2101 tcgcgcgcaa gcgcggatgc ccgggagggt taataaatgg cggacggagc ggggacaggc
     2161 tccgcccgcg aacgaaaaaa cacgaaatta ccatgcacgc gcgatcgtta aacccagccc
     2221 cggcgcgccg cggcggagcg cgggcgttca ccctgatcga attgttggtg gtgatcgcga
     2281 ttattgcgat cctggcggcg ctgctgctgc ccgccctggc ccgcgccaag gacaaggcgc
     2341 aggcggccgg gtgccttaac aacctcaagc aactccaggt cggctgggtg atgtacagcg
     2401 atgacaatgt caacgtcctg attccgaatg cgccgtcggg ctttccgccc tggcagacct
     2461 ggtgcagcgg gacgacggtg gggtggggca atgtcaatgc caacaccaat cgcacgccct
     2521 acctgcaatc cctgatggcc ccctacatgg tcaaccagat cggggtttac cgctgcccgg
     2581 gcgataacgt gccctccttc aacgggcggc gtctccgcag ctactccatg aattgccaca
     2641 tgggcagcat ctactccggg ccgctgaccc aatcgtataa ccccggctgg aaggtgtatc
     2701 agaagatcac cgatattacc cagcccgcgc cggcggatgc gtttgtgttt atcggcgaac
     2761 acgccggcag catcaacgac ggctatctgc aggtgcgcag caacgcgccc gagtttcccg
     2821 acgtgccggg ttcctaccac ggcaagagcg agggcgtttc ctatgccgat ggccacgccg
     2881 cgctgcataa atggtggacg gccgcgctgg cggtgcccgt cgcgtatggg gtcgcctaca
     2941 aatccgtgaa agtggccgcg gacaacgcgg attggatttg gctgcgcgac cacgccagcg
     3001 ttaaggagta atccgctccg ccggggcccg cgccggcgaa accgggcggc gcaggtattc
     3061 gtggatggcg cgcgcggcgc gtttgccgtc gcccatggcc aggataaccg tggcggcgcc
     3121 gcgcacgatg tcgccaccag cgaacacccc cgggaggttg gtcatgccct gttcgtccac
     3181 ggcgatgccg ccccgggaat ccagcttgag ctccgggcag gtgccggcca ggagcgggtt
     3241 ggcgcgggtg ccaatggcca cgatcaccac ctcgcagggc accaggaact ccgagccggg
     3301 aatggcacgg ggacgggcgc gcccggaggc gtcgggctcg cccagttcca tgcggacgca
     3361 ctggaggccg gtgacccaat tccgcgcgtc gccctggatg gccaccggcg cggcgaggaa
     3421 ctcgaactgg atgccctcct cctcggcgtg aagcacctcc tcggcccggg cgggcatctc
     3481 cttgcgggtg cggcgataga cgatcgtcgc ggactccgcg cccaggcgtt tggcggtgcg
     3541 gacggcgtcc agggcgacgt tgccgccgcc gatcgtggcc acgcgccggc ccgtgaggat
     3601 gggggtgccg gcgcgggcgg accggcgggc gcccatgagg ttggcgcggg tgagatattc
     3661 gttggcggaa tacacgccct tgaggttctc gccggggatg ttcatgaaca ccggcaggcc
     3721 ggcgccgttg gcgatgaaga cggcgtcgaa gcgcgcgcgc agctccgcca gcgtgtaagt
     3781 ccggccaatc acggtgttgc aaaggatctt cacgcccgcc cgctcgagcc gggcgacttc
     3841 ctcgcgaacg atctgcttgg gaaggcgaaa ctcggggatg ccatagacca gcacgccccc
     3901 gggcttgtgg agcgcctcga agacggtgac ctggtggccg agccgggcga gctccccggc
     3961 ggcggtaagc ccgccggggc cggcgccgac gattccgacc tgcttgccgg tggccggggc
     4021 cgcctcgagg gccgggcgct gggcgtggcc gcgcgcccag tcgccgagaa agcgttccag
     4081 gtggccgatg gccaccggca gccccttgta gccgcggatg cactccagtt cgcattgggt
     4141 ttcctgcggg cagacgcgcc ccgtgaccgc gggcagcgga ttgtcgttca gcaggcaatg
     4201 ggcggcttcg ggcagcttgc cctcggtcag caactggatg aagcggggaa tgttgatccc
     4261 gacggggcag ccggcgacgc acttggggtc tttgcacaac aggcagcgct cggcttccag
     4321 gagggcgatc cgctccggca ggcccaggtt gacttccgcg aaattggcgc ggcgaacttc
     4381 gggcgcctgc gccggcatgg gctggcgctc aatctgcagg cgctgccggg tggaaagttt
     4441 cggggacacg gggcctcctt tagctggcaa cggggctccg cccgccccgg gggcggcgtc
     4501 cgctcggtgc cgcgcccggc gcggccgcgg gaagcgcggg gcggtttccc cgcgccgcag
     4561 cccgggagcg gcggcctatg gaatcagcgt cgcccaagtt tgcagtcccc ttcctggcag
     4621 gcggcgagcg ctcgctgttc aaagtcccgg taggtggtca ggcggtccat caactgctcg
     4681 aagtccaccg cgtgcccgtc aaactcgggg ccatccacgc aggcaaaccg cgtctgcccg
     4741 cccacgctga cgcggcagcc cccgcacatg ccggtgccgt ccaccatgat agggttgagc
     4801 gaaacgatgg tgcgaacccc cagcggccgg gtcaggttcg ccaccgcgcg catcatcggc
     4861 acggggccca ccgcatagac gatgtcaatg ccgccggcgg ccagcgcgtc ccgggcggcg
     4921 tcggtgacga atccgcgccg gccgtagctg ccgtcgtcgg tgcaaacgat cacctcgccc
     4981 agccggcgca gttccggctc gaagatgatg gcttcccggg tgcgcccccc gataatgctg
     5041 ctgaccgtga cgccccggcg cttcaggccc tgggcaatcg gatgcaccac ggcgaccccc
     5101 accccgcccc cgatgcacag cgcgcggccg gcggcgatca gttccgtggg gtggccgagc
     5161 ggcccggcga tgttggcgat ggcctccccc gggttcagcg cggccagctc gcacgtgctc
     5221 tggcccgcga cctggatgac cagcgtgatg gtgcccgcct cggcgtcggc gtcggcaatc
     5281 gtcaggggaa tgcgctcggc gtcgccgccg cggtgcacaa tcacaaactg ccccggctgc
     5341 cggatgcgcg cgatgcgcac ggcggcgacg tccagccgga tgatctgcgg gctcaacgat
     5401 gctttggcaa tgatcttatg catgccgatc ggccaatctt ataaacaggc gtagtcctgc
     5461 ggacaatctg cacctgaatc cgcgttcccg caaccgccgg acgcgcggcg atggcaccgg
     5521 gcggggcgga tcgggcgatg ggcgggcggg cgcttagcgg cgccgggggc cgagggggat
     5581 ttcaaacgag ccgcccaccc acaacttgct gtccgagttg ttgccatcca gcaccgagcg
     5641 caggtgaaag gcatagcgcc agtgccgttt cgcggtggta tagctgaagc ccgcctcgag
     5701 cgcgtcgctg atctcccgaa cgtcccgggg ataatccagg gcggcgggct gggcggggtc
     5761 gtagcggata ttgtcgcccg ccacggagtg cagccgccgg tagcccacgt ccagttccca
     5821 tcccttgatt tgctgaaaca gtcccaccgc gatgaggtat tggtcattgc cggtcgtgcg
     5881 attccagcga tacagcgcgt ccccatagac gcccagcccc ggccagccaa agtgcttccg
     5941 cagcagcagc tccggctcga tcgccgccga acgctgcccg ggggcgaagg ggaagttctt
     6001 gtcatacgac cccggcagca ccgccccggc gcggaacgtc agggtcggca cccagggcga
     6061 ttgccgcggc gcggcctcgt tgaagatctg gtagcggacg ccaaaggacc agtccatcag
     6121 cccggtggtg gaatcggcat gttcgtcggg cgcgaacttg cgccagccga cggtggtggc
     6181 gcccacgctc aaatcggccg cccaccgttc ggtaatgcca tactgcgcgg tcaggtagcc
     6241 ctggttgata tcaatgccgt atttctcgcc gtgcgagcgg gtcgtgatat cgtgctgccg
     6301 gccccccgcc cagatgtggt aggcctcgga ttcctcatac accagcccga tcacaaactg
     6361 gccccgctcc ggcacgttgg cccactgcgc aaacggccgg tgcggcacgt tttcggaagc
     6421 ccgcagcccc ggcgtcagcg ccaggatgcc caggatcact cccagcccca gggccgcgcg
     6481 cccaacccgc cgcccgccaa ccggaaaact gaacttgcaa tagctcatca taaatgtttg
     6541 cccggattat gccggaatcc gcgccccggc gcaatcctcg aaacggcccg cgtcggcgcg
     6601 cgcgcgcgga aacccgcttg cctggcgccc gccggacggg aataatcccc ccaaccgtga
     6661 tgaacgctat cgtttccaaa actcctgcct gccgatgaac gcaaatcctc ccgatcgccc
     6721 cgcccgctcc tcgcggcgcg cctttctcca aacctccagc acggccctgg ccggcgcggc
     6781 gctgtccggc gtcctcgccc gcccgggcta cgcggcggaa aacaacacgc tcaagctggc
     6841 cctcgtcggg tgcggtggac gcggctcggg cgccgccgcc caggcccttt cgacccgcgg
     6901 ccccacccgg ctgtgggcgg tggcggacgt cttcgcgcac cgcgtccaga cctgcctggc
     6961 cggactcaag tcctcgcacg ccgcccaggt ggacgtgccg cccgaacgcc agttcacggg
     7021 cctggaggga ttccgccagg ccatggacac gctgggcaag ggcgacgtgg tgctgctggc
     7081 cactcccccg gccttccgcc cccgccacct ggaatacgcc gtgcagaagg gcctgcatgt
     7141 gttcatggaa aaatccttcg ccgtggacgc gccgggcatc caccgcgtct tgcgcgccgg
     7201 cgcggaagcc acggccaagg ggttgaagat cgtcggcggc ctgatgagcc gccactaccg
     7261 gccgctcgaa gaggccattc aaaagattca tgacggcttg atcggcgacc tggtcacgtg
     7321 ctgggcctac cgcatgcacg gccccgttgc cctcaatccc cgcgccgccg gcctgacgga
     7381 actggcccac caaatcgcca attacagttg cttcacgtgg ctcaacggca gcttcctcga
     7441 agactggctc atccacaacc tggatgtctg ctgctgggcc aagaacgcct ggcccgtctc
     7501 cgtccagggc atgggcggac gccaggtccg ccaggaaccc gaccagctct tcgatcacta
     7561 caccgccgaa tactccttcg ccgacggcac gcgcctgctg gcccagggcc gccacatgac
     7621 caactgctgg gacttcttcg gcaacctcct ccacggcacc cgcggcagcg gcatcctcgg
     7681 cgaaggccag ccccgccccc gcctctaccg ggactggcgc cccgccgcgg aaaacctcgc
     7741 ctggagctac accggccccg cctgcgaccc ctaccaggcc gagcatgacc tcctcttcag
     7801 cgccattcgc aacgacctgc cctgcaacga aaccgagcgc tgcgccaaag cctgcttcac
     7861 cgccatcctg ggccgcatgg cgtgcgagtc cggcaagcgc gtcacctggg acgaagcgtt
     7921 cgcctccacc accgagctgg ccccgggact ggaccattac accctggact ccaccccgcc
     7981 ggtcgtccct gatgccgcgg gccgctaccc gcttgccctc cccggccaga cgccgccctt
     8041 ttgaacgccg ccgccccgat ccgcccccgt cccgccggcc ggcgccccgc cccggcgcgc
     8101 cgcgcattta tcggtttccg aaaagcggga atcgtgtatg ttaaggcgcg gcgccttcct
     8161 gaccctggtg cagtgaaagg cgggaacggt catccggtgg cgacccggtc attttccgga
     8221 tccgcccccg ccgccgacca cgaataacta tgcgcaagtc gaagcctgaa aagaaacgcc
     8281 ggcccgtaag caaaaaggcc ccgtcccgcg cctcccggcg cgcccccgct ccgacggctc
     8341 cgcccgcgcg cgctccgcgc cgccgctccc gccgccccgc cgcggcaaca cccgccgctc
     8401 ccgctcccgc ccccgcgtcc ccgccgcccg ccctggacct cgcccagaag gtcaaagaac
     8461 tcctgcgcct cgccaaggaa cagggctacc tgacctacga cgacctgaac gacgcgctgc
     8521 ccgatgaact cgtcaccccg gcggacctgg accaggttct gctcaaactg cgcggcctgg
     8581 acgtcgaaat cattgatgcc gccgaagtgg accacgggcg gcgccccgac ggggaggagg
     8641 agggcgagga aaagcgctac gacctgctgg acgacccggt gcggctgtac ctgcgccaga
     8701 tgggcaaggt gcccctgctg acgcgcgagc aggaagtcgc catttgccag cgcatcgaag
     8761 ccgccgaagc ggaagcccgg cgcatcctgc aaagcttcgg gctcgccccc aaggaatacc
     8821 tcgccctggc ggaaaagctg ctggccacgc cgccccggga acgctttgat cgcgtggtcg
     8881 cggaccagtc ccccctccac cgcaagcgct acctccactc cctccggcgc ctggtcaagc
     8941 gcgtccgcgc cctggatttc caggcggacg cccggttcgc cgcgtggcag cgggccaccc
     9001 ggaaagccga ccgcgcgcgc ctgcattacc agcttcagcg cttccaccgg aagctcgacg
     9061 cgctctatcc ccgcttcggc ttcaagcgca aggtggtgga cgacatggcc ctggtcgtcg
     9121 aaaacctgca cctcaaactg caggccagcc tccaggccct cgccgctccc ccggcccgcc
     9181 agaaccccgc cgcccgccgc gccttcctcc gggccgagca cgacaaaatc cgcatgtggg
     9241 agcgctttgc gcgcatgccc cacgcggact tcctcacggc gcgcgcgcag ttgaacgacg
     9301 cccgcgcgcg cgcgcaggag gccaagcggg agatggtgga agccaacctg cgcctcgtca
     9361 tttccatcgc caagaaatac accaaccgcg gccagtcctt cctcgacctc attcaggaag
     9421 gcaacgtcgg cctcatgaag ggcgtggaga aattcgaata ccgccgcggc tacaagttct
     9481 ccacctacgc cacgtggtgg attcgccagg ccatcacccg ctgcatcgcc gaccaggccc
     9541 gcaccatccg catccccgtc cacatgatcg aggtcatcaa caagctctgg cgcacgcaaa
     9601 agcaactcac ccaggagctg ggccgcgaag ccaccaccga agaactcgcc gacagcatgg
     9661 aaatgcccct cgaacgcgtg cgcgtggtcc tcaaactggc ccagcagccc gtctccatgc
     9721 agacccccgt cggcgaggcc gacgacgcca gcttcggcga cctgcttgaa gacaagtccg
     9781 ccgaaaaccc cggcgacgtc accagcttcc acctcctccg cggccggctc tccgaagtcc
     9841 tcggcagcct ctccgaacgc gagcggcgca tcctcgaact ccgctacggc ctccacgacg
     9901 gcttcccccg caccctggag gaagtcggcc agcaatacga agtcacccgc gagcgcatcc
     9961 gccagatcga agccaaggcc ctgcgcaaac tccgccaccc cgtccgcaaa agcaaactcg
    10021 aaggcttcct cgaatcctcc cccgccccgc gcctggcctg agcggcccct cgaattggcg
    10081 attggaaacc cggccccctt cggctacttt ccgcgccatg aacattttgg acgaactcca
    10141 atggcgcggc ctgctggcgg attgcaccga cgccccgggc ctggcccggc ggctcgcggc
    10201 cggccccctt accgtctatt gcggctttga ccccaccgcc gacagcctgc acgtcggcca
    10261 cctcgtgccc ctgctggcgc tgcgccgctt ccagcgcttc ggccaccacc ccctcccgct
    10321 cgccggcggc gccaccggct ccatcggcga ccccagcggc aaatcccagg aacgccagtt
    10381 gctcacccgg gaaaccctcg ccgccaacat cgcccaggtc aaacgccagc tccagcgcct
    10441 gctcgacttc gactcccccc ccaaccccgc ccgcctcctc gacaacgcca cctggaccgc
    10501 ccccctcagc tacctcgact tcctccgcga cgtcggcaag cacttctccg tcaaccagat
    10561 ggtcgccaag gaaagcgtcc gcgcccgcat gcaggaccgc gacgccggca tcagctacac
    10621 cgagttcagc tacatgctcc tccaggcctt cgatttctac gtcctctgcc gcgactacca
    10681 gtgcgaactc caaatcggcg gccgcgacca gtggggcaac atcaccgccg gcattgactt
    10741 cacccgcaaa aaactcgccc gcaccgtctt cggcctcacc ctgcccctca tcaccaacgc
    10801 cgacggctcc aagttcggca aaaccgtcgc cggcgccgtc tggctcgacc ccgcccgcac
    10861 cagcgtctat cgcttctacc agttctggat ccgcgccgac gaccgcgacg tcatccgcta
    10921 cctcaaatac ttcaccttcc tgagccgcga cgaaatcgcc gccctggagc aacaacacac
    10981 cgccaacccc ggcgcccgcg ccgcccacca ggccctcgcc cgctccgtca ccgaactcat
    11041 ccacggcccc gacgccaccg ccgaagccat ccgcgccagc caaatcctct tcggcggcga
    11101 cctcgccggc atctccgccg ccgtcttcaa cgaaatcgtc ggcgaagtcc cctcccggcc
    11161 cctcaaccgc cgcgagctcg aaggccccgg caaacccctc gtcgaactgt tcgtcctcag
    11221 cggcctcgcc ccctccaaag gccaggcgcg caaagacatc gaagccggcg gcgcctacct
    11281 caacaaccgc cgcgccacca accccctgca ccccgtgacc accgccgacc tcctcttcaa
    11341 ccgccacctc ctcctgcgca aaggcaaacg caactacgtc gtcctctccg ccgcctgacc
    11401 ccgcccgtga tcctctacaa cctcttcccc ctcctggccg gccccctcca ccgctgggag
    11461 cctcacctcg accgcgcccg cgccctcggc ttcaactggc tcttcctcaa ccccatccaa
    11521 ctccccggcg cctccggcag cctctactcc atcgccgact acttccgctt caacccccgg
    11581 ctcctcaacc ccgccgaccc cgctcccccg gaaacccaaa tccagcgcct ggccgcccgc
    11641 gcccgcgaac gcggcctgcg cctcatgatt gacctcgtca tcaaccactg cgccattgac
    11701 tcccccctca tccgcgagca cccccagtgg ttcgtccgcc agcccgacgg ccgccccgcc
    11761 cacccctcct gtcagcacca cgaccagact gtcgtctgga ccgacctggc ccagttcgac
    11821 caccagcacc cgcgcgaccc cgagggcttc taccgctact gccgccgcgt cgtggacgcc
    11881 ctgctcgccc tcggcttcga cggcttccgc tgcgacgccg cctaccaaat cccccgcccc
    11941 ttctggcagc gcctcatcca cgacacccgg caacgccacc cccacgcctg cttcgccgcc
    12001 gaaaccctcg gctgctcccc cgcccaaacc ctcgaaaccg cccgcgccgg cttcgactac
    12061 gtcttcaaca gctccaaatg gtgggacttc cacggctgct ggcttctcga acaatacaac
    12121 ctcatccgcg aaatcacccg ctccatcagc ttccccgaaa gccacgacac cccccgcctc
    12181 gccgccgaac tcaacggcaa cccccacgcc ctcaaacaac gctacctctt cgccgccctc
    12241 ttcgccgccg gcgtcatgat gcccatcggc ttcgaattcg gcttccgcaa acccctccac
    12301 gtcgtcaact cccaacccgc cgactgggaa cccgtccacg tggacctctc cgacttcatc
    12361 acccgcgtca accacctcaa actccaacac cccgtcttcc gcgaggaaag ccccgccagc
    12421 atcctcccca ccgacaaccc caacatcctc ctcctctgga aagcctcggt gcaacacccc
    12481 caggaagctc tgctcatcct taacaaagac ccccatcacc accaggactt ccacaccgac
    12541 cacttccgcc gcttcatcca gtccggcgcc cccttgcgcg acgtctcccc cgaataccct
    12601 ctcgattacc tccccgaacc cttccactac gccctccgcc ccggccaggg catcgtcctc
    12661 gtcaccaccc gttcctgaac cgccctcaaa cccaacccaa ataccgcccg ccctcgcgca
    12721 gtaacaacaa cgcaaaccca cccagcaaca ccgccaagca acgcaacccc acccggtaca
    12781 cccgccccgt catccaatca cgagaccgcg ccgccagcaa cgccaccgcc accttgatcc
    12841 ccaccagcca cccgtaaaac cccagcaaaa acgcccccgc cgccagccac ccccccgcca
    12901 gcgccttcgt caggctcgcc atccccaccg tcagccagaa cagccacgga tgcggactca
    12961 gcagattcgt gcccagcccc ttgctccacg accgcggcga cgcctccgcg cacaccaccc
    13021 cgcgccgcgc cgcaacccag ctctcccccg ccagatacaa aagaaaaacc ccgcccgcca
    13081 ccgaaaccca ccccagcaac cggtcaaact ccgtcaaccg ggccgccagc gccatcgtca
    13141 ccccaatcac cggcacatcc gccagcaacg gactcaacgc caccttgcac ccctcgcgcg
    13201 cgccgtgccg cagcgtctgc gccagcacca gtgccagcaa aggccccggc atcaccccgc
    13261 acgaaatccc caaaaacgac cccacaacca aactcgtcaa caccgcccca tcttaaccca
    13321 cccgctccgc gccgcaacga aaacacccgc cccggcaaac
//
LOCUS       DAOPQG010000097        12906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5638_length_12906_cov_17.908957, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000097 DAOPQG010000000
VERSION     DAOPQG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5638_length_12906_cov_17.908957"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(492..1565)
                     /locus_tag="PKI07_10655"
     CDS             complement(492..1565)
                     /locus_tag="PKI07_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51550.1"
                     /translation="MKKYSVLVVGMGKRGMHHAAAFNANPRFQVTGICDIDPKRLDDA
                     AARLGQPQKGTDAAALARAVKPDVFCFCTLPNLRTPLIEAALAGGAQLIAFEKPVTLT
                     SAELFTIRDRLRKAGVKAVVSHQHRYGRHYQKVKEIIASGALGRVHTVYGSSPGWMTH
                     MLSHLIDYTCWFNQYAPGAWAMAQAAGRAKFGDNHPSPDYLGGFVQFANGVRGIYECG
                     AGAPDQPEVAKWWGKNRIGAQGTEGFAEVLTNGGWRAVTRSGGAQSGDGAMNYDLDMP
                     PYIQEMADWLDDPQKVHPCNFDHAALGAEIMFALQRSAAEGGQVALPLTAGADEQALL
                     KARLADRPVLVSCELNKKEFGLA"
     gene            complement(1708..2892)
                     /locus_tag="PKI07_10660"
     CDS             complement(1708..2892)
                     /locus_tag="PKI07_10660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC51551.1"
                     /translation="MNVAFYGHVRQYRNIKSEIDKNIKTVLESGQYVMGPMLKKFESE
                     LAAYHGTKYAVGLGNGTDAIWLALMALGIGPGDEVITHPNTFFATAEAIWIAGATAVF
                     VDCQAGSKCICPGRIEAAITRKTKAIIPVHLYGQCADMTAISKIARKHKLKVIEDNAQ
                     AIGARGPKFRIGQLSDAATTSFIIQKNLGTFGDGGALVTNNAAIDAAVRRLRNHGSTA
                     RNVHSFGFNSRLDDLHAGILSAKLKHIDEYNDARRQWAARYTQGLKDCRTFDLPVELK
                     GYRHVFHLYVIEVKKPQWRDQLLDYLVKNGVDAKTHYSIAIHQQRGYPWGKKARIVGS
                     VANAEANAACCISLPMFPELTAKEVDYVIAKLKAWDAKYAHLAPAGKGPAQGGACGCS
                     CK"
     gene            3031..3534
                     /locus_tag="PKI07_10665"
     CDS             3031..3534
                     /locus_tag="PKI07_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOC51552.1"
                     /translation="MNLPYKVATLLYCFSEGGEILLLERAQEPNRGRWSPCGGKLRMA
                     AGESPYACACREAREELGLALTPRDLWLTGLASEHGYEGQAHWLMFLFEVRPRLRQLP
                     PAHREGRFQFFTRAALAELPIPQTDRERIWPWFWQHRGGFFAAHCHCHPDGRHEWTLE
                     ESRIAHA"
     gene            3527..4012
                     /gene="tadA"
                     /locus_tag="PKI07_10670"
     CDS             3527..4012
                     /gene="tadA"
                     /locus_tag="PKI07_10670"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOC51553.1"
                     /translation="MPDEPVIDLQSDSYFMGEALRQAARAAAAGEVPVGAVIVRAGRV
                     IARAFNQVELLKDATAHAEMLAVTQAEAVQGDWRLTDCTLYVTKEPCPMCAGAIVHAR
                     LARVVYGAHDPKAGAAGSALNLLQFPTLNHRCAITGGVHEAECRALLQLFFAAQRKKE
                     P"
     gene            4073..4846
                     /locus_tag="PKI07_10675"
     CDS             4073..4846
                     /locus_tag="PKI07_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC51554.1"
                     /translation="MSPTESKYVENKAYLARLAREARLGARENWLRQATEFLPEEQWF
                     RHVKQDFPKYVPFLMKQCELEFRGRILEIGAGGAWLSAELSKLPRVVAIMATDLSPYP
                     LKEYAPRVFELRHAHARKITRMASDFHRLDFPDHYFDYVVCSSVLHRATNIVLALREA
                     KRVLKPGGQLVVIREPVWPLVRIKSRARILAKLLATGINDHFYTLSDYREFFKQAALP
                     MQAQKVNLSRGFKYYVDSVVNGLTHARYAFIGTRRGRSS"
     gene            4887..5216
                     /locus_tag="PKI07_10680"
     CDS             4887..5216
                     /locus_tag="PKI07_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51555.1"
                     /translation="MNHSLTAWKEAAAEAALTAFLNREIKSLGVVRELAIRTREKTLR
                     IAVDLKGEAARLWIEAAAYELSERDGEVYLVVRGITASREWIAAALNQYVAGRAFRLP
                     PAARFLR"
     gene            5288..7702
                     /locus_tag="PKI07_10685"
     CDS             5288..7702
                     /locus_tag="PKI07_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51556.1"
                     /translation="MMASDRKHVSGAFSRRTFLKSASLTLGGLGLARYGRLEGWAGAA
                     PAAAPAALGAADLPRGSAPAPVPLPHFPDRLHAFVWRNWQLVPARRMAEVVGTSASRI
                     VRLGRSMGLAGPPRITAEQQRRSALTVIRRNWHLLPYDQLLQLLGWTAEEMAFSLRED
                     DFLFIKLGSLKPACERLRYRPPEAAARARAREMAAVLRAEFSGGVGHSGQPLFAFVRQ
                     LSQPPRAAAAPSGASAFSPRFCYSYFALYGDPLLDPTLDPYPDGYLARLAAAGVEGVW
                     LQAVLTRLAPFPWDARQSQHYEARLRNLRRLVERARRRGVGVYLYLNEPRSCPVSFFA
                     TRPGLKGVPAGDFAALCTSVPEVRAWLASAVASICRAVPGLAGFFTISGSENLTNCWS
                     HGGGQGCPRCGQRSPAEVIAELNTTFYEGIRQAGHGQKLIVWDWGWHDGWVEKIIAQL
                     PPEVAFMSVSEWGMPIKRGGVATAVGEYSISTIGPGERARQRWQQARARGLRTVAKTQ
                     CGNTWELSAVPYIPALANVARHIANLRQQGVNGLMLGWTLGGYPSPNLEVAAELGSAP
                     AGSPAAEPEHALARVAERRFGTALAAPMVRAWQDYSAAFSEFPFHGGLVYSAPMQVGP
                     ANPLWGEATGYRATMVGFPYDDLDAWRAVYPPEVFIEQFEKVADGFERAHASLAAACQ
                     ACRRELTPAQAAAAGEELDVGEAAALHFRSTANQARFILARRRLQAAGSTAEAQAAGV
                     ELEELLRAEMRLARRLHVLQTRDSRIGFEASNQYYYVPVDLAEKVINCQDLLTRWLPA
                     ARRRHG"
     gene            7907..8086
                     /locus_tag="PKI07_10690"
     CDS             7907..8086
                     /locus_tag="PKI07_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51557.1"
                     /translation="MKPKAKKTKAEVPFDPVLFAATVPARKPTPQDIADAKEHEDDAI
                     EPQQYDSMEDMVKDL"
     gene            8083..8349
                     /locus_tag="PKI07_10695"
     CDS             8083..8349
                     /locus_tag="PKI07_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51558.1"
                     /translation="MIFRRAVRFKREFKALPPDIQRVARAKFALFSSNWRHPSLRYHK
                     LEGLTWHGHQVFDLWVTEHYRVLFVIDHDVIVSFSIGPHGIVSK"
     gene            8513..10345
                     /locus_tag="PKI07_10700"
     CDS             8513..10345
                     /locus_tag="PKI07_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOC51559.1"
                     /translation="MHLQLIDWLIVLATLLICFVPALFFGKRAGRNTTEFFVSGRAVP
                     WWLAGLSMVATTFSSDTPNLVTDIVRQRGVAGNWVWWAFVLTGVATVFFYARMWRRSE
                     VMTDLEFYEIRYSGKAASIVRGFRSIYLGLFFNCVILATVCLAACKIANILFGLPRWQ
                     TLLLCGLLNVVFAAHSGLWGVLVIDMLQFFIKMTAVIAAAFFAVQHVGGLSVMVERLS
                     APRLAPDGQTLLHYLNVLPDFSSNWDLVVAVFIMPLAVQWWATWYPGSEPGGGSYIAQ
                     RLLASRSERDSLGAVLFFNVAHYVLRPWPWILVGLCSLLVYPELADIQKAFPNLDPHL
                     LGHDIAYPAMLKFLPVGFIGLMVGGLIAANSSTVLTHLNWGASYLVHDFYRRFLNRGA
                     AEQHYVRAGRLTTVGLFVCAGGMVYLLDSAKSAFDLILQIGAGTGLLYLLRWFWWRIT
                     AWCEVVAMISSFGISLVLLILKTQCGVAISTHYALLLTIAVTTVCWMVTAFVGPQTDR
                     QTLIEFYRKVRPFGPGWRRIREAAGVSAAEAAATRENIPLALLGWSAGCAVIWSSLFA
                     VGNFLYGRIGLALWLSGVFIACGGVLLYVINQLWAGQSPASNSR"
     gene            10342..11979
                     /locus_tag="PKI07_10705"
     CDS             10342..11979
                     /locus_tag="PKI07_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOC51560.1"
                     /translation="MNRLIALMLLSLTAGHALAAGDSPALIPAPRKLECREGTFNLHP
                     KTHILADRASQDTARYLAERLRQATGYKLKTTLGTEPRKAKGAIILTTQEAKAALGPE
                     GYELIVAPDEVALRASGQAGMFYGVQSLLQLLPPQIFAAKPEPGQAWQAPCVWIEDQP
                     RFKWRGVLFDTARHFFPKAEVKQLLDVLALHKVNMLQLHLTDDQGWRVEIGRYPRLTQ
                     VGAWRDEAGFGLDPKLSTTYGPDGRYGGYYTKADIWDIVAYAAARHITVVPEIEMPGH
                     ASAALAAHPELSCSGGPYTPNAKGGVFAGVYCAGKEETFEFLQNVLAEVCELFPGKYI
                     HIGGDEVPKDNWKQCPRCQARIRQEGLEDERELQSYFIRRIEKFINAQGRTLIGWSEI
                     REGGLAQNAVVMDWIGGAAEAASTGHDVIMSPTSHCYLDYYQSTNHATEPKAIGGYVP
                     LSKVYAFEPIPPNLAPAHQSRLLGAQGNLWTEYIPNFKHAQYMLFPRLCALAEVVWSP
                     PGARNWEDFLRRLPVQFQRFDQLGVNYRKGTPERIGE"
     gene            12211..12855
                     /locus_tag="PKI07_10710"
     CDS             12211..12855
                     /locus_tag="PKI07_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC51561.1"
                     /translation="MIVLTNTGGVAMTNSFLIADETARQAVLFDAPDHTVAPLLETAT
                     ARGWELTGLWLTHGHFDHFADHAVVRQKFPGARILLHALDQPKARQPEAQTRLFGLPL
                     VIPPLVADADLTDNQELKIGSLAVVALHTPGHSPGHVAYYFPQEQLLVGGDLIIGGSI
                     GRTDLPDSDPRQMQASVRRVMSLPGATRLLGGHGQPTTLRQEQEHNLLVQELLA"
BASE COUNT         2029 a         3996 c         4436 g         2445 t
ORIGIN      
        1 acaccttgag tggaaaccat gataaccaat ttgctgaact tgacggacac aactcttgta
       61 caccccttgt acacggcctg atagtagggt aaatgcaggg gaaatgcagg gtaggttcaa
      121 ggctcgccac agccgggata agttggattt tgtctccgtt tagcccattc catcgtttgt
      181 aagttattga ttttacaagt aaaaggtgct tggggttcag cagcgatgcc gctgaattcg
      241 tcaaacaagg ggttgccagg gctatggtct gggcggtcag ggctgcccgg gcctcgatgg
      301 agcgagggcg ggcggttgaa ttaccccgat gaagcgttcc ggtttatgta ttgcaggtgc
      361 ttttggggcg ttcgttggcc cttttgccag ccgccggtgt atttggggcg ccggggctta
      421 atcaaaaaac cgcctgttta gggtttttag cccgtggcag gcggttttcg gcgggaactc
      481 gtggccgacg gttaagccag tccaaactct ttcttgttta gctcgcagga gacgaggacg
      541 gggcggtcgg cgagccgggc tttgagcagg gcctgctcgt cggccccggc ggtcagcggg
      601 agcgcgacct ggccgccttc ggcggcgctg cgttgcaagg cgaacatgat ttcggcgccc
      661 agggcggcgt ggtcgaagtt gcaagggtgg accttttggg ggtcgtcgag ccagtcggcc
      721 atttcctgga tgtagggcgg catgtcgagg tcgtagttca ttgcgccgtc gccggattgg
      781 gcgccgccgg agcgggtgac ggcgcgccag ccgccgttgg tgaggacttc ggcaaagccc
      841 tcggtgcctt gcgcgccgat gcggtttttg ccccaccatt tggcgacttc gggctggtcg
      901 ggcgcgccgg cgccgcactc gtagatgccg cgcacgccgt tggcgaactg gacgaagccg
      961 ccgaggtagt cgggcgaggg gtggttatcg ccgaacttgg cgcgcccggc ggcctgggcc
     1021 atggcccacg cgcccggggc gtattgattg aaccagcagg tgtagtcaat caggtgggag
     1081 agcatgtggg tcatccagcc gggggagctg ccatagacgg tgtgcacgcg gcccagcgcg
     1141 ccgctggcga tgatttcctt caccttctgg taatggcggc cgtagcggtg ctggtggctg
     1201 acgacggctt tgacgccagc tttgcggagc cggtcgcgga tcgtgaacag ctcggcgctg
     1261 gtcagggtga cgggcttttc gaaggcgatg agttgggcgc cgccggccag cgccgcctcg
     1321 atcagggggg tgcgcaggtt gggcagcgtg caaaagcaga acacgtccgg ttttaccgcc
     1381 cgggccaggg cggcggcgtc ggtgcctttc tgcggctggc ccagccgggc ggcggcatcg
     1441 tcgaggcgtt tcggatcaat gtcgcagatg ccggtgacct ggaatcgggg gttggcgttg
     1501 aaggcggcgg cgtggtgcat gccgcgtttg cccatgccga cgacgagaac cgagtatttt
     1561 ttcataagcg ggtatggcga gagatggcaa tcagggtgcg gggattggaa gttgtagggc
     1621 gcggagggtc tggcggacgc gggtggtgga gggaaggcgg gcgccgggga ggaatcccgc
     1681 ggcgcccggc tcgagttcca atgcgggcta cttgcaactg cagccgcagg cgccgccctg
     1741 ggcggggcct ttgccggcgg gggccaggtg ggcgtatttg gcgtcccagg ccttcagctt
     1801 ggcgatgacg taatccactt ccttcgcggt gagctcgggg aacatcggca ggctgatgca
     1861 gcaggcggcg ttggcttcgg cgttggcgac gctgccgacg atgcgggctt tcttgcccca
     1921 cggatagccg cgctgctggt gaatggcgat cgagtaatgg gtcttggcgt ccacgccgtt
     1981 cttgacgagg taatccagca actggtcgcg ccactggggt ttcttgacct cgatgacgta
     2041 gaggtggaag acgtggcggt agcctttcag ctcgaccggc aggtcaaagg tccggcagtc
     2101 cttcaggccc tgggtgtagc gggccgccca ctggcggcgg gcgtcgttat actcgtcaat
     2161 gtgcttgagt ttggcgctca ggatgccggc atggagatcg tccaggcggc tgttgaagcc
     2221 gaagctgtgc acgttgcggg ccgtggagcc gtggttacgc aagcggcgca cggcggcatc
     2281 aatggcggcg ttgttggtga ccagcgcgcc gccgtcgcca aaggtgccga ggttcttctg
     2341 gatgatgaag ctggtggtgg cggcgtcgct caactggccg atccggaact tcggcccgcg
     2401 cgcgccgatg gcctgggcgt tgtcctcgat caccttgagc ttgtgtttcc gggcaatctt
     2461 gctgatggct gtcatgtcgg cgcactggcc gtagaggtgg acggggatga tggccttggt
     2521 cttgcgcgtg atggcggcct cgatccggcc cgggcagatg cacttgctgc cggcctggca
     2581 atccacgaac acggcggtgg ccccggcgat ccagatggct tcggcggtcg cgaagaaggt
     2641 gttggggtgc gtgatgactt cgtcgcccgg gccaatgccc agggccatca gcgccagcca
     2701 gatggcgtcg gtcccgttgc ccaggccgac ggcgtacttg gtgccgtggt aggccgccag
     2761 ctcgctttcg aacttcttga gcatggggcc catgacgtat tggccgctct ccaggacggt
     2821 cttaatgttt ttatcaatct cggatttgat attacgatac tgacggacgt ggccgtaaaa
     2881 cgctacattc atggggcgaa agactacttt tccgaaatgg ggacacaagt gtttttttgg
     2941 attccgacga aaaaaatcgc cgccgcgggg ggagggggct aggatggctg gcggcgtgaa
     3001 acgaatgggc aaacctggag taaggccggc atgaacctgc cgtataaagt ggcgacgctg
     3061 ttgtattgct tcagcgaggg cggggaaatc ctgctgctgg agcgggcgca ggagcccaac
     3121 cgggggcggt ggagcccctg cggcggcaag ctgcgcatgg cggcgggtga atcgccctac
     3181 gcctgcgcct gccgcgaggc gcgggaggag ctggggctgg cgctgacgcc ccgggatttg
     3241 tggctgaccg gcctggccag cgagcatggc tatgaagggc aggcgcactg gctcatgttt
     3301 ttgttcgagg tccggccgcg gctgcgccag ttgccgccgg cgcaccggga ggggcgcttc
     3361 cagtttttta cgcgggcggc actggcggaa ttgccgatcc cgcagacaga ccgggagcgc
     3421 atttggccct ggttctggca gcatcgcggc gggttctttg ccgcgcattg ccattgtcac
     3481 ccggacgggc gccacgagtg gacgctggag gaatcccgga ttgcccatgc ctgacgagcc
     3541 ggtcattgat ttgcagagcg attcgtattt catgggcgag gccctgcgcc aggccgcgcg
     3601 ggccgcggcc gccggggagg tgccggtcgg ggcggtgatc gtgcgcgcgg ggcgggtcat
     3661 tgcccgcgcg ttcaaccagg tggaattgct caaggatgcc acggcgcatg cggagatgct
     3721 ggccgtgacg caggccgaag cggtgcaggg cgactggcgg ctgacggact gcacgctgta
     3781 cgtcaccaag gagccgtgcc cgatgtgcgc gggggccatc gtgcatgcgc gcctggcccg
     3841 cgtggtatac ggcgcccacg acccgaaggc gggcgcggcc ggcagcgcgt tgaacctgct
     3901 gcagttcccg acgctgaacc accgctgcgc aatcaccggc ggggtgcacg aggcggaatg
     3961 ccgcgcgctg ctccagttgt ttttcgcggc gcagcggaaa aaggagccgt agccggcggt
     4021 gcaaaacgcg gttgcgcgcc cgcggcgggt ttcctatact gccggagtcc ggatgagccc
     4081 aaccgagtcc aaatacgtcg agaacaaagc ctacctggcg cgcctggcgc gcgaggcgcg
     4141 gctgggcgcg cgcgagaact ggctgcggca ggccacggag tttctcccgg aggaacagtg
     4201 gtttcggcat gtgaagcagg actttcccaa gtacgtgccg tttctgatga agcagtgcga
     4261 actggagttt cggggccgga ttctggagat tggcgcgggc ggggcctggc tgagcgccga
     4321 actttccaaa ctgccccggg tggtggccat catggcgacg gatctctcgc cctacccgct
     4381 caaggaatac gcgccgcggg tcttcgagct gcgccacgcc catgcccgga agatcacgcg
     4441 gatggcgtcg gacttccacc gcctggattt tcccgaccat tatttcgact acgtggtttg
     4501 ctcgtctgtg ctgcatcggg cgacgaacat cgtgctggcg ctgcgcgaag ccaagcgcgt
     4561 gctcaaaccc ggcgggcaac tggtggtcat tcgcgagccg gtctggcccc tggtgcgcat
     4621 caagtcccgc gcccggatcc tggccaagct gctggccacg ggcatcaacg accacttcta
     4681 cacgctgtcc gattaccggg agttcttcaa acaggccgcc ctgcccatgc aggcccaaaa
     4741 ggtcaacctg tcccgcggct tcaaatacta cgtggatagc gtggtcaatg ggttgaccca
     4801 cgcccgctac gccttcattg gcacccggcg cgggcggtcg tcctgagccg ccggcgcgcg
     4861 gcggcgcggc gagctgcgaa cctgaaatga atcattccct caccgcgtgg aaggaagccg
     4921 ccgcggaagc ggccctcacg gcgttcctca accgcgaaat caagtccctg ggcgtggtcc
     4981 gggaactggc catccgcacc cgggagaaga cgctgcggat tgcggtggac ctgaaagggg
     5041 aggcggcgcg cctctggatc gaggcggcgg cgtacgaact gagcgagcgg gacggggagg
     5101 tttacctcgt tgtgcggggc atcaccgctt cccgcgaatg gattgcggcg gcgttgaacc
     5161 aatacgtcgc cggccgggcc tttcggctgc cgccggccgc ccggtttctg cggtgaccgc
     5221 cggcgccggg gaggcaaacg gcattgcctc cgcgcgggca aagggttacc aatcagcatc
     5281 aataattatg atggcgtcag accggaagca tgtaagcggg gcgttcagcc gccgaacctt
     5341 tctcaagtcg gcctccctga ccctgggcgg cctggggctg gccaggtatg ggcggctcga
     5401 gggctgggcc ggggcggcgc ccgccgcggc gccggccgcc ctgggcgccg cggatctgcc
     5461 ccgcggcagc gcccccgcgc cggtgccgct gccgcacttc cccgaccgcc tgcatgcgtt
     5521 tgtgtggcgc aactggcaat tggtgcccgc ccgccgcatg gcggaagtcg tcggaaccag
     5581 cgccagccgg attgtgcgcc tgggccggag catggggctg gcggggccgc cgcggatcac
     5641 tgcggagcag cagcgccggt ccgcgctgac ggtcatccgc cgcaactggc atctgctgcc
     5701 ctacgaccag ctcctgcagc tcctgggctg gacggcggaa gaaatggctt tctcgctgcg
     5761 cgaggatgat ttcctgttca tcaagctggg cagcctcaaa ccggcatgcg aacgcctgcg
     5821 ctatcgtccg cccgaggcgg ccgcccgcgc gcgggcgcgg gagatggccg ccgttctgcg
     5881 cgccgagttt tccgggggcg tcgggcactc cgggcagcct ttgtttgcgt tcgtccggca
     5941 gttatcgcag cctccccggg ctgccgcggc tccgtccgga gccagcgcct tttccccgcg
     6001 gttctgctat tcctattttg ccctctacgg ggatccgctg ttggatccga cgttggatcc
     6061 ctaccccgac ggctacctgg cgcggctggc cgccgccggg gtggaggggg tctggctgca
     6121 ggcggtcctg acgcggctcg cgccgttccc gtgggatgcg cggcagagcc agcactacga
     6181 ggcgcggctg cggaatctgc gccggctggt cgagcgggcc cggcggcggg gggtgggcgt
     6241 ttatctctac ctgaacgagc cgcgttcctg cccggtgagt ttctttgcca cgcgcccggg
     6301 tttgaaaggc gtgccggcgg gtgactttgc cgccctgtgc accagcgtgc ccgaagtgcg
     6361 cgcgtggctc gcgtcggcgg tggcctccat ctgccgcgcc gttcccgggc tggcgggctt
     6421 cttcaccatc agcgggtccg agaacctgac caattgctgg tcgcacggcg gtggccaggg
     6481 atgcccgcgc tgcggccagc gttcgcccgc cgaggtgatt gccgagttga acaccacctt
     6541 ttacgaaggc atccgccagg ccggccacgg ccagaagctg atcgtctggg attggggctg
     6601 gcacgatggc tgggtggaga agattatcgc gcagcttccc cccgaggttg cgttcatgag
     6661 cgtcagcgaa tggggcatgc cgatcaagcg cggcggcgtg gccacggccg ttggcgaata
     6721 ctcgatttcc accatcggcc ccggcgagcg cgcccggcag cgctggcaac aggcccgcgc
     6781 ccggggcctg cgcacggtgg ccaagacgca gtgcggcaac acctgggaac tctcggcggt
     6841 tccgtacatc ccggcgctgg ccaatgtcgc ccgccacatc gccaacctcc gccagcaggg
     6901 cgtcaacggc ctcatgctcg gctggacgct gggcgggtat ccgtcgccca acctcgaagt
     6961 ggccgcggaa ctggggtccg cgcctgccgg cagcccggcg gccgagccgg agcacgccct
     7021 ggcgcgggtg gcggagcgcc ggtttggaac cgcgctggcg gcgccgatgg tgcgggcctg
     7081 gcaggattac agcgcggcgt tcagcgagtt tccctttcac ggcggcctgg tctatagcgc
     7141 gccgatgcag gtcggcccgg ccaatccgct ttggggcgag gcgaccggct atcgcgccac
     7201 gatggtcggc tttccctacg acgatctgga cgcctggcgc gccgtctatc cgccggaagt
     7261 ctttatcgag caattcgaga aggtggcgga cggcttcgag cgcgcgcacg ccagcctggc
     7321 cgcggcctgc caggcctgcc gccgcgagtt gaccccggcg caggccgccg ccgcggggga
     7381 ggagttggac gtgggcgaag ccgccgccct gcatttccgc agcacggcca accaggcccg
     7441 cttcatcctc gcgcgccgcc ggctgcaagc ggccgggtcg acggccgagg cgcaggcggc
     7501 cggggtggaa ctcgaggaat tgctgcgcgc ggagatgcgc ctggcgcggc gcctgcacgt
     7561 cctgcaaacg cgcgactcgc ggattggctt cgaggcctcc aaccagtatt actacgtgcc
     7621 ggtggacctg gcggagaaag tcatcaactg ccaggacttg ctgacccgct ggctgccggc
     7681 ggcgcgccgc cgccacggat aggagcagag ggtgaggggt gctcctcatc cctgcccatg
     7741 ccgcatctgg taccaccacc ggcccgccca tagactccat cctgaagctg aaactgaacg
     7801 gttcggccat ggacctgggg gcgctctgag ttgctttgtg gcccaacatg acgatgccgg
     7861 gttctggtgt tgagttggag cggccgttgg gctagcttgg gttatcgtga agcccaaagc
     7921 gaaaaagacg aaggcggaag tcccattcga tccggtgctg tttgcggcga ccgtgccagc
     7981 gcgcaaaccc acccctcaag acatagctga tgctaaagag cacgaagacg acgccatcga
     8041 gccccagcag tacgactcga tggaagacat ggtcaaagac ctatgatctt ccggcgcgcc
     8101 gtccggttca aacgcgagtt caaagcactt ccgcctgata tccaaagagt cgcgcgggcc
     8161 aagttcgcct tgttcagctc gaattggcgg catccctcgc ttcgctacca caaacttgaa
     8221 ggcctcactt ggcacggcca tcaggtcttc gacctctggg tgaccgagca ctaccgcgtc
     8281 ctgttcgtga ttgaccacga cgtcatcgtg tctttttcca tcggcccgca cggcatcgtc
     8341 tcgaaatagc gcagggcggg gtcgccccat cctgaaaatt gagcccggcg gctcggcgat
     8401 ggacctggag ccgccggacg gccttatacg ccggacttgt tttcggcgtg ggagggcgtt
     8461 agcgttttcg gccgctggcg ttgaagacgc cacggatccg aagaactctg gtatgcattt
     8521 acagctcatt gactggctga tcgttctggc caccctgctc atttgttttg tgccggcgct
     8581 gtttttcggc aagcgcgcgg ggcggaacac cactgaattc tttgtctcgg gccgggcggt
     8641 gccgtggtgg ctggctgggt tgtccatggt ggcgaccacg ttcagcagcg atacgcccaa
     8701 cctggtgacc gacatcgtcc ggcagagggg cgttgccggc aactgggtgt ggtgggcgtt
     8761 tgtgctgacg ggcgtcgcga cggttttctt ttatgcccgg atgtggcggc gctcggaggt
     8821 gatgacggac ctggagtttt atgaaatccg ttactccggc aaggcggcca gcatcgtgcg
     8881 cgggttccgt tcgatctacc tggggctgtt tttcaactgc gtcatcctgg ccacggtctg
     8941 cctggccgcc tgcaagatcg ccaatatcct ctttggactg ccccgctggc agaccctgct
     9001 gctgtgcggc ctgctgaacg tggtgtttgc cgcgcactcg gggctttggg gcgtgctggt
     9061 cattgatatg ctccagtttt tcatcaagat gacggcggtg attgccgcgg cgtttttcgc
     9121 ggttcagcat gtcggcgggc tgtcggtgat ggtggagcgg ctctccgcgc cgcggctcgc
     9181 cccggatggc cagaccctgc tgcattatct gaacgtgctg cccgacttca gcagcaactg
     9241 ggacctggtg gtggcggtgt tcatcatgcc cctggcggtg cagtggtggg cgacgtggta
     9301 tccgggttcg gagccgggcg gcggcagcta cattgcgcag cgcctgctgg cttcccggtc
     9361 ggagcgggat tcgcttggcg cggtgctgtt ctttaatgtg gcgcattatg ttctgcgtcc
     9421 ctggccgtgg atcctggtcg gcctgtgttc gctgctggtg tatccggagc tggcggacat
     9481 ccagaaagcc tttcccaatc tcgaccccca cttgctgggg catgacattg cctacccggc
     9541 catgctcaag tttctgccgg tcgggttcat cggcctgatg gtgggcgggt tgatcgccgc
     9601 caactcttcg acggtgctga cccatttgaa ctggggcgcc tcctacctcg tgcatgactt
     9661 ttaccggcgg tttctcaacc ggggcgcggc ggagcagcac tatgtgcggg cgggccgcct
     9721 gaccaccgtg ggcctgtttg tgtgcgccgg cggcatggtt tacctgctgg attcggccaa
     9781 atccgccttc gacctcatcc tccagatcgg ggcgggcacg gggctgttgt acctgctgcg
     9841 ctggttctgg tggcggatta ccgcctggtg cgaagtggtg gcgatgatca gctcgttcgg
     9901 catttccctg gttctgctaa ttctcaagac gcaatgcggc gtggcgatca gcacccatta
     9961 cgcgctcttg ctgaccatcg ccgtcaccac ggtgtgctgg atggtgaccg ctttcgtcgg
    10021 cccccaaacc gaccgccaga cgctgatcga gttctaccgg aaggttcggc ccttcggccc
    10081 cggctggcgg cgcatccgcg aagccgcggg tgtttccgcg gcggaagccg cggccacgcg
    10141 cgagaatatc ccgctggcgt tgctgggctg gtcggcgggc tgcgcggtca tctggtcgtc
    10201 gttgttcgcg gtgggcaatt tcctctatgg ccggatcggc ctggcgctgt ggttgagcgg
    10261 cgtattcatc gcctgcggcg gggttttgct ctatgtgatt aaccagctct gggccggcca
    10321 aagcccggcc tcgaactccc gatgaaccgc ctaatcgctc tgatgctgct ctctttgaca
    10381 gcgggccacg ccttggcagc cggcgactct cccgcgctaa tcccggctcc tcgcaaactg
    10441 gagtgccggg aaggaacctt caaccttcac ccgaaaacgc atatcctggc cgaccgcgcc
    10501 tcccaggata cggcccgcta tctggcggaa cgtttgcgcc aggcgaccgg ttataaactc
    10561 aagaccaccc ttggcactga gccgcgaaag gccaagggcg cgattatact caccacccag
    10621 gaggccaagg cggccttggg cccggaaggc tacgaactga tcgtggcccc ggatgaggtt
    10681 gcgctgcgcg cctccggcca ggcgggcatg ttctacggcg tccaatcttt gctgcaactg
    10741 ttgccgccgc agatcttcgc cgcaaaacct gagcccggcc aggcatggca ggcgccttgc
    10801 gtctggatcg aggatcagcc gcgcttcaag tggcgcgggg tgttgttcga cacggcccgc
    10861 cacttctttc ccaaagccga agtcaaacaa ctgctggatg tgctggcgct gcacaaagtg
    10921 aatatgcttc aactgcatct gaccgatgac caggggtggc gcgtcgagat tggcaggtat
    10981 ccccgcctga cccaggttgg cgcatggcgg gatgaggcgg gattcggcct ggacccgaag
    11041 ctcagcacca cttacggacc ggacgggcgg tacggcggct actacaccaa agcggatatt
    11101 tgggatatcg tcgcctatgc cgcggcccgg catatcaccg tggtgccgga gattgaaatg
    11161 cccgggcatg cctccgccgc cctggccgcg cacccggaat tgagttgcag cggcgggccc
    11221 tacacgccca acgccaaagg cggagtcttc gcgggcgtct attgcgccgg caaagaggaa
    11281 accttcgagt tcctccagaa cgtcctggcc gaggtctgcg agctgttccc gggcaagtac
    11341 atccacatcg gcggcgacga agtgcccaag gacaactgga agcagtgccc ccggtgccag
    11401 gcgcgcatcc ggcaggaagg attggaggac gagcgcgaac tgcagagcta cttcatccgc
    11461 cgcatcgaaa agttcatcaa cgcgcaaggg cgcacgctga tcggctggag cgaaatccgc
    11521 gagggcggcc tggcccaaaa cgccgtcgtc atggactgga ttggcggcgc ggcggaagcc
    11581 gccagcacgg ggcatgacgt catcatgtcc cctaccagcc attgctacct ggattactac
    11641 cagtccacca accatgccac tgagccgaaa gccatcggcg gttatgtgcc gctcagcaag
    11701 gtctatgcat tcgagcccat ccccccgaac ctggccccgg cgcaccagtc gcgccttctc
    11761 ggcgcgcagg gcaacctgtg gacggaatac attcccaact tcaaacacgc gcaatacatg
    11821 cttttccccc gcctctgcgc gctagccgag gtggtttggt cgccgcccgg cgcgcgcaac
    11881 tgggaggatt tcctgcgccg cctcccggtt cagttccagc ggttcgacca actcggcgtc
    11941 aactaccgca agggcacgcc tgagcgcatc ggcgaatagt ttcaggcgca tcaggcctcg
    12001 gctgacggtt cgctgccgaa tgccgtcctt gcctcgtttc gcccgcaatg ggtgccgcca
    12061 ctgccgggaa acccgcgggc cgcgaactca tcttgaatgg gcgcgcgccc cggtattcaa
    12121 ataaccgggc cgccgttgcc tgcaaatacg tccgtaagcc gccagccttt gcggctggct
    12181 gtcttcaccc cggacggcta cacttcgggc atgatcgtac tgacaaacac cggcggggtc
    12241 gcgatgacca atagctttct catcgcggat gaaaccgccc ggcaggcggt gctttttgac
    12301 gcccccgacc ataccgtcgc gccgctcctc gaaacggcga cggcgcgcgg gtgggagctg
    12361 acggggttat ggctgacgca tgggcatttc gatcacttcg ccgatcatgc ggtcgtgcgg
    12421 cagaagtttc ccggcgcccg cattctgctc catgcgctgg accagcccaa ggcgcggcag
    12481 ccggaggcgc agacccggct gttcgggctt ccgctggtta tcccgccgct cgtggcggac
    12541 gcggacctca ccgacaacca ggagcttaaa atcgggtcgc tggcggtggt ggccctccat
    12601 actcccggcc attcgccggg ccacgtcgcc tactacttcc cgcaggaaca actgctggtt
    12661 ggcggcgacc tgatcatcgg cggctcgatt ggccgcaccg atctgccgga ttcagacccg
    12721 cggcaaatgc aggcctccgt ccgccgggtc atgagcctgc cgggcgcaac gcggctgctc
    12781 ggcggccacg gccagcccac cacgctgcgg caggaacagg agcacaatct gctggtgcag
    12841 gaactgctcg cctgatacca tttcctgtta gtagttgcat tgtattgaga ggcatggcaa
    12901 gaacag
//
LOCUS       DAOPQG010000098        12740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5731_length_12740_cov_15.507765, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000098 DAOPQG010000000
VERSION     DAOPQG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5731_length_12740_cov_15.507765"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(522..1355)
                     /locus_tag="PKI07_10715"
     CDS             complement(522..1355)
                     /locus_tag="PKI07_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51562.1"
                     /translation="MIQFAPEPGSREAFDAFLRHLLAANWEAADGPMRFSPDLTLSDL
                     ADSVFLQNTRRFLAALADAGSTPATVTGNLTRAFVRQMFDRLILPAHFRDITPRFRKV
                     LNEPDVWRLHIARIICEIAGLVARRNQRFQLTRAGRELRAEAHAGTLYRKLFLAYFRK
                     FDLHYGFHSRNVPGIQQTLAVTLWRLDNVACDWTPVRGLAGKILLPGVLNQLQAEIVS
                     VHNEEEWILSGYVLEPLLDLGLLDRNPRNDWPLTENCQVRITPLWQKFIHFDHWAGGG
                     Q"
     gene            2028..6188
                     /locus_tag="PKI07_10720"
     CDS             2028..6188
                     /locus_tag="PKI07_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOC51563.1"
                     /translation="MKINTSFRRHINYRQRLAAGLLLLVLGAGSLGRSLAETHLLPSP
                     SPNPWGDWEVWAKVEGQDVKCFGEGEQGDSESHYRVILYTVPKEATVTVYADFHGSLP
                     EGVYPVSQEIGMTPGAGWTVAGGVAVSTVNLVTNQEVLAQRHMIQGVVTLHTTDPNYS
                     PSRGFLADLFVARPIGVSAGGSSGGALALYAHSQVPCMATNPITHVSWADASLSPLPD
                     AEVKAEAIEGVEVSSGWLTTGELGRAMATVRAPASSIPDAVGLVSGRIRIKAVKDRAT
                     VQREVDIHIPYASVAGAYCASLIGRAGAILEPQPIVDGDNLRPGDVVQVGNEFLGNSP
                     YLNLWFCNGQRVVLQSDTVGGIRAVVGQGSLDQRTPVFLATLENVVQNIQADPRRYGR
                     MMVYKAIGNAVDGLLGIPDPVGWTITTPGGAVENWVGNYVEGAYQPQKRSPSGGHSKS
                     GSQDSSSPDAAWAGSVVGFYSDGTARAYNRGATLNLHGPSASGAAPLNGMLAARLDAP
                     GGPISSPGLAPRDGFAPFISFEPTNGASGVPIRPRLRAQFLNFGINTVLPGSLFCRLD
                     GRLLQPQERLNTSEFVWQTPAAEALTPGLHQWDIEFALLQGGLLRTSVTFSVTEALPA
                     PRTVRAAAGLQRVALRWDAEALAWARGGFRVYRAAPGNPPVLISGSAPLRQPNFVDLA
                     PLASGNYEVAGLDAAGNEGPRSAPVTVLFPGSAPPVPQAVAVTVQAREAQPGFALGID
                     DFTPGFTLWRIEAATNAGGPFTNVLEGELTSLALWPVPRPFEETRPWYRVTALNVDGV
                     AGPPVTAGPLALPVALPGVAGLTALPNSNGTVQVRWDAWTARAILGYRVERGFDGKWT
                     LAADVDASTQTWTETQSGDGVRRQWRVRARLAGGALSPASPAVEFCLQPVPAHPGNIR
                     FASATVTGAEGESLPIRIVREGGSDGPAFVTWSSWGWAGSATPEADYTPGAGLLIFAP
                     GETEKTVNVALQADNLREQPAESFYVYLKQVEGGPGLAEPSITQAFITDGPELAWESI
                     WLYASENDPPEVRFHVRLDPPANHPVSVGYEFQAGGSTATPGADFTGPLTGTLTFAPG
                     QTNQFFAVQLINDTLKEGTQPETVQYRLTNPQGAAIDDRDPFRLYATLEIRDDDTRPG
                     RAVFADRTMRLREGESRAITLRREGGADGELGVFLFGMGGNAQLETDWAINPNFPSFA
                     DGQTNLSVTLSALTDAETEGAEMAILGAQAMGGFHQMATLFVVIEDATTPASGFAAWA
                     APRLAAFPAEQRGPYADPDGDGAPNWAEFLWRTNPAQPDRPAPPQSRFTEWGEWLLTV
                     TVTEDPALLVVAEFSKDVTWTEPEFLAGNWETNGDGTRTGTFHCFNFGGSAGFIRFRG
                     EWCGSPQP"
     gene            complement(6251..7825)
                     /locus_tag="PKI07_10725"
     CDS             complement(6251..7825)
                     /locus_tag="PKI07_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOC51564.1"
                     /translation="MPGYFALILHAHLPFVRHPEHAQFLEENWLFEAVAETYLPLLRL
                     LEGWRRDGLSPRLTLTLTPTLCAMLQDPLLQDRCERYLREQVALAEKEIQRTRWEKPL
                     QQVARFYHERFTAAQALYAGHGRDLVRAFRQFQESGHLEIITSAATHALLPLLADHPP
                     SLRAQVRVACDHYQHCFGCEPRGIWLPECAYVEALEEVLQAADLRWFVTDTHGLLHAR
                     PRPRYAVFAPIFTPKGIAAFGRDPECSRQVWSRQAGYPGDPRYRDFYRDIGFDLDLEY
                     VAPHLAAPGHRGFTGLKYHRITGRAPHKEIYDRAAAVHAAGEHARHFLAARRAQFQRL
                     GEALDRPAIVVAPYDAELFGHWWFEGPEFLDALVRQAGAEPGAFTFITPGEFLRRHPT
                     HQAATPAASSWGEGGYWRVWLNEKNEWIYPHLRAAQERMTELARRFPRPDAIQERALK
                     QAARELLLAQASDWPFLLRSETRPGYARKRVREHLLRFTRLYEQLAGEPLDREWLRQV
                     EERDNLFPDLNYRYWA"
     gene            complement(7881..9269)
                     /locus_tag="PKI07_10730"
     CDS             complement(7881..9269)
                     /locus_tag="PKI07_10730"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOC51565.1"
                     /translation="MKKKKPRLATAAPAARRTVRRAARPKKSPLRRPARPARGKPARA
                     RRPKKPAPVARRKAAPAASPAEKVKKTVRRRPRTPAPRRARAPRTGARTVSRRAAAPG
                     PERMPAARPPRPAAPAVLPSPAAPDPLIPPLLLEEDEPPCPPLRGAEPTPGPEPAAAG
                     DPLESRGAALPGSYGTGRLVLTAREPRWLYASWDLAARQQRQYNARSADGHLVVRVHA
                     GSIAERPAAEVHVHPESRHWFIQVSHAGAQYVAELGYYRPRRRWVQIATSAPVTTPVD
                     TVSADRTVRFVTIPPQVRLTQLATPARPAAPGKEPPPETAREDAPAEMLAWTPELGDA
                     LSSWAEGEAALWAVAGGEAGSVSSPAAGAAEPPAPGFWFRVHTDLVIHGATEPDATVT
                     IGGRPIPLRADGTFSCRLSLPDGEHSASVAALSARGDLRQARLRVTRATDYPDGSGAD
                     ASEPASRPGEEP"
     gene            9436..9512
                     /locus_tag="PKI07_10735"
     tRNA            9436..9512
                     /locus_tag="PKI07_10735"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9470..9472,aa:Gly,seq:gcc)
     gene            9569..10651
                     /locus_tag="PKI07_10740"
     CDS             9569..10651
                     /locus_tag="PKI07_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOC51566.1"
                     /translation="MNKLRSNLEQNLGGVALLVLGIGCLVVLRPFVSALLWAVVLCFS
                     SWPLYQRLLRLLGHRATLAATLMTLVMVLVLLLPFVLIGTTLGNSIKDLTLATRNWIQ
                     DGPPHPPAWLARVPVVGATAVDYWQSLAQDTARLWAQAQRFIEPVSAWLIRAGLALGG
                     GLLELALSIFIAFFLFRDGVSLARRLAAAIGRIGGERGYYLLGVAGQTVRGVVYGILG
                     TALAQAIMAGLGYLLAGLPGPGLLALLTFFLSVVPVLGTSLVWGPAAFWLFNQGSTGW
                     AIFMIIWGLGVASLDNFIKPWLISQGSNLPFILIFFGVLGGALAFGFIGVFLGPTLLA
                     VGYRLVEEWSAGARETAAGGETPRPE"
     gene            complement(10687..12078)
                     /locus_tag="PKI07_10745"
     CDS             complement(10687..12078)
                     /locus_tag="PKI07_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOC51567.1"
                     /translation="MNAKDLIRLGVPPGEATRRATDFISRFILGGGDRARLTEEVAAI
                     VANPGSFTADPLRGAFARALQSAPPPRAAPARYRQWGEGLEAEAVRQMERACQLPVAV
                     AGALMPDAHVGYGLPIGGVLATENAVIPYAVGVDIACRMKLTVLDLPLRELDRPLERL
                     ARALAAETRFGVGSNFKPRRWHEVMDADWSVSPVTRENKDRAWAQLGTSGSGNHFVEF
                     GRFTAHEKIQSLEPGTYVALLSHSGSRGTGAAVCDYYSRLAARQLPRLPGELKRLAWL
                     PLDTAAGQEYWAAMELMGRYAAANHALIHRHIAAHLGAQVRLDLENHHNFAWKERHRV
                     DGVEREVVVHRKGATPAGRGVLGIIPGSMATPGFVVSGKGNAESLNSAAHGAGRVMSR
                     TQAHRTFDWKTVKHYLRARGVTLLSAGLDEVPMVYKNIHNVMAAQSDLVSVLGQFDPK
                     LVKMSPPGERAED"
BASE COUNT         1971 a         4278 c         4367 g         2124 t
ORIGIN      
        1 cccagtggct taccatgacc cgctgctttt tgcagaactt ttaggacact accgatttat
       61 gcaccccagc attggtggtt ctttggctgt cctggctgct tcttcagtcg ggagaaatca
      121 gggtccagac aggagagcca ctcgtcggcg cgcgggcggc ggacctgctg ctgttagtgg
      181 aagtctgaaa gcgggccaga ggcccgcgcg ccgggcttgc agtgataaaa tgcctgggcg
      241 gatttgtgta acccggcgtt ggtggttctt tggctgtcct ggctgcttct tcagccgggg
      301 gaaaccaggt tccggatagg agggcaaccc gtcggcgcgc gggcggcgga cctgctgctg
      361 ttagtggaag tctgaaagcg ggccagaggc ccgcgcgccg ggcttgcagt gataaaatgc
      421 ctgggcggat ttgtgcaacc tggcgcgggt ggcggaccca ctggctcccg gcgcagggtg
      481 agcatcttcc cgcggctaac gtctgccttg ggagcggggg ttcattgccc gcctcccgcc
      541 cagtggtcga agtggatgaa tttttgccac aagggcgtga tgcgcacctg gcaattttct
      601 gtgagcggcc agtcgttgcg ggggttgcga tccagaaggc ccaggtcgag cagcggctcc
      661 agcacatagc cgctcagtat ccattcttcc tcgttatgca ccgatacgat ttcggcctgg
      721 agttggttga gcacgcccgg caacaggatt tttccggcca ggccgcggac gggcgtccag
      781 tcgcaggcca cgttgtcgag gcgccagagg gtcacggcca gggtttgctg gatgccgggc
      841 acgttgcggg aatggaagcc gtagtggagg tcgaacttgc ggaagtaggc gaggaagagc
      901 ttgcggtaga gcgtcccggc gtgagcctcc gcgcgcagct cgcggcccgc ccgggtgagt
      961 tggaagcgct ggttccgccg ggccaccagg ccggcgattt cgcagatgat ccgcgcgata
     1021 tgcagcctcc agacatccgg ctcgttcagc accttgcgga accggggcgt gatatcgcgg
     1081 aagtgcgcgg gcagaatcag ccggtcgaac atctgccgga cgaaggcgcg ggtgaggttg
     1141 ccggtgacgg tggcgggtgt gctgccggcg tccgccagcg cggcgaggaa ccggcgggtg
     1201 ttttgcagga agacggagtc cgccaggtcg gacagcgtga ggtccgggct gaaccgcatc
     1261 gggccgtcgg cggcctccca gttggcggcc agcaggtggc gcaggaaggc gtcaaaggcc
     1321 tcgcggctgc cgggttcggg agcgaattgg atcatagcgg gtcaggctgg ttcgcggagg
     1381 tggacgaggt tgcggcaggg ggcggatggg gtcatggggt tcgggagccg caccccggaa
     1441 aggcgggcgg gcgttgaatc cgtaacctgg cggcggcgcc actcatgacg aaaggttggt
     1501 gcgtaacggt tcgggcagcc ccacagcggc gccgcctctt tgcgcttaac gcaaccgtag
     1561 cgtgcccact cccgcgcgca acataaatat accggcaagg acacgttttc cacagggctt
     1621 attgggcggg gcctgctgta ttttatcggc cctggccaac gctgtgttcc atgccggggc
     1681 ggcctccggc ggagcgcggc gcgaatgggt tagcggacgg caggccgctc cccggggccg
     1741 aggcgcaacc tccggcgatt cttatccggt cgcgcggcat gggagggcgc cccaaccctt
     1801 ttcggccggc ttccgccgcg ccgggacgga tccctgggca acggggcgcg ggattaatta
     1861 attttggcct tgccaaatgt ccccgtttag atcacagtgg cggcaagttt ctggctgctg
     1921 gatatatgaa tcatccgcgc cttagcgcac cgttgccacc tggcaaacag atggcgcgag
     1981 tccggcttta gccagctaaa gggttccccc ctcgcacttc ataccttatg aaaattaaca
     2041 cttcgtttcg acggcacata aattatcggc agcgcctggc ggccggcctg ctgcttcttg
     2101 ttttaggcgc cggctcgctg gggcggagtc tcgcggagac ccatctgctc ccatccccaa
     2161 gtcccaaccc gtggggagac tgggaggtct gggcgaaagt ggaggggcag gatgtgaaat
     2221 gttttggcga aggcgagcag ggggattcgg aatcccatta tcgggtgatc ctttacacgg
     2281 tgcccaaaga ggccacggtc accgtgtacg ctgattttca tggttcgctg ccagaggggg
     2341 tgtatcccgt cagccaggaa atcggcatga ctcccggcgc cgggtggact gtggcgggcg
     2401 gggtggccgt ctcgacggtt aatctagtca ccaaccagga agtgctcgcc cagcggcata
     2461 tgatacaggg cgtggtgacg cttcatacga ccgatcccaa ctattcgccg agcaggggct
     2521 ttcttgccga cttgtttgtg gcgcgaccca ttggcgtgag cgcgggcggc agttcgggcg
     2581 gcgcgctggc tctctacgct cattcccagg ttccctgcat ggccaccaac ccgatcaccc
     2641 acgtctcgtg ggcggatgcc agtctttcgc cgctgccaga cgccgaggtg aaagccgaag
     2701 cgatcgaggg agtggaagtt tcctccggtt ggctgaccac cggggaactc ggccgggcga
     2761 tggctacggt ccgggcgccc gccagttcca tcccggatgc ggtggggctg gtgtcgggcc
     2821 gcatccggat caaagcggtg aaagatcgcg cgacggtgca acgggaggtg gatattcaca
     2881 ttccctacgc ctcagtcgcc ggtgcctatt gcgcctcgct gatcgggcgc gcgggcgcga
     2941 tcctcgagcc gcagcccatc gttgacgggg ataacctgcg gccgggggac gtggtgcagg
     3001 tcgggaatga attccttggc aacagcccgt atctgaatct ctggttctgc aatggccagc
     3061 gggtggtgct gcaatcggac accgtgggcg gaatccgggc ggtcgttggc cagggcagcc
     3121 tggatcagcg cacgccggtg ttcctggcga cgctggagaa cgttgtgcaa aacatccagg
     3181 ccgatccccg ccgttacggg cggatgatgg tttacaaggc cattggcaac gccgtggacg
     3241 ggctgctcgg cattcccgat ccggtgggct ggacgatcac cacccccggc ggcgcggtgg
     3301 aaaactgggt cggcaattac gtggagggtg cctatcaacc gcaaaagcgc tccccctccg
     3361 gcggccactc gaaatcgggg tcgcaggaca gttcgtcgcc ggatgcggcc tgggcgggca
     3421 gcgtggtggg cttttacagc gatggcacgg cgcgcgcgta caaccgcggc gccaccctga
     3481 acctgcacgg gccgtcggct tccggcgccg cgcccctgaa cgggatgctg gcggcccgat
     3541 tggacgcgcc cggcggcccg atttcgtccc ccggcctggc gccccgcgac ggctttgccc
     3601 cgttcatctc tttcgaaccg acgaatgggg caagcggcgt gcccatccgg ccccggctcc
     3661 gggcgcaatt cctgaatttt ggcattaaca ccgtgctgcc cggcagtctt ttttgccgtt
     3721 tggatggccg gttgctgcag ccgcaagagc ggctcaacac cagcgagttt gtgtggcaga
     3781 cgccggcggc ggaagcgctc acgcccggcc tccaccaatg ggacatcgag ttcgcgttgt
     3841 tgcagggagg gttgctgcgc accagcgtca ccttcagcgt cacggaagca ttgccggctc
     3901 cgcgcacggt gcgggcggcg gcggggctgc agcgcgtggc gctccgctgg gatgcggaag
     3961 cgctggcctg ggcgcggggc ggcttccgcg tgtaccgggc cgctcccggc aatcctccgg
     4021 tcctgatcag cggctccgcg ccgttgcggc agcccaactt tgtggatctg gcgccgctgg
     4081 ccagcggtaa ttatgaagtc gccggactcg acgccgcggg caacgaaggg ccgcggagcg
     4141 cgccggtgac ggtgctcttc ccgggcagcg cgccgcccgt gccgcaggcg gtcgccgtga
     4201 cggtgcaggc ccgcgaggcg cagcccgggt ttgcgctggg cattgatgat ttcaccccgg
     4261 gcttcacgct ctggcgcatc gaggccgcca cgaatgccgg cggcccgttc accaacgttc
     4321 tggagggcga attgaccagc ctggcgctgt ggccggttcc gcggccgttc gaagagacgc
     4381 gcccgtggta ccgcgtcacc gccctcaacg tggacggcgt cgccggcccg cccgtgacgg
     4441 ccgggccgct ggccctgccc gtggcgcttc cgggcgtggc cggcctgact gccctcccca
     4501 attccaacgg cacggttcag gttcggtggg atgcctggac cgcgcgggca atcctcggct
     4561 accgcgtcga gcgcgggttc gacggcaagt ggacgctggc ggcggatgtg gatgcgtcca
     4621 cgcaaacctg gacagagacg caatccggcg acggcgtccg gcggcagtgg cgcgtgcggg
     4681 cccggttggc cggcggcgcg ttgtcgcccg cctcgcccgc ggtggagttc tgcctgcaac
     4741 ccgtccccgc gcacccggga aacatccgtt tcgccagcgc gaccgtaacg ggcgccgagg
     4801 gggaaagtct gccgatccgc atcgtccgcg aaggcggcag tgatggccct gcctttgtca
     4861 cctggtccag ttggggttgg gccggaagcg ccacgcccga ggccgactac actcccggcg
     4921 cgggcctgct catcttcgcg cccggggaaa ccgaaaagac cgtcaacgta gcgctgcagg
     4981 cggacaacct ccgggagcag ccggcggaga gcttctacgt gtatctcaaa caggtcgaag
     5041 gcggtcccgg cctggcggag ccatccatca cgcaagcctt catcacggac gggcccgaac
     5101 tggcctggga atccatctgg ctgtatgcgt ctgaaaacga tccgcccgaa gtgcgcttcc
     5161 acgtgcgcct ggatccgccc gcgaatcatc ccgtcagcgt gggctacgag tttcaggccg
     5221 gcggcagcac cgccaccccc ggcgcggact tcaccggtcc cctgaccggc acgctgactt
     5281 ttgcccccgg acaaaccaac caattctttg cggtccagct cattaacgac acactgaagg
     5341 agggcacgca acccgaaacg gtgcaatacc ggctgaccaa tccccagggc gcggccattg
     5401 acgaccggga tccgttccgg ctgtatgcca ccctggaaat ccgcgatgac gacacccggc
     5461 cgggccgggc cgtttttgcg gaccggacga tgcggttgcg cgagggcgag agccgcgcga
     5521 tcacgctgcg gcgcgagggc ggcgccgacg gcgaactggg cgtattcctc tttgggatgg
     5581 gcggcaacgc gcaactggaa acggattggg ccatcaaccc gaatttcccc agttttgccg
     5641 acgggcagac caacctgagc gtgaccctca gcgcgttgac cgacgccgaa acggaggggg
     5701 cggaaatggc cattctcggg gcgcaagcca tggggggatt ccaccagatg gcaaccctgt
     5761 tcgtagtcat tgaagatgcc accacgcccg cctccggatt cgccgcctgg gcggcgccgc
     5821 ggctggcggc gttccccgcg gaacaacgcg gcccctatgc cgatccggat ggcgacggcg
     5881 cgcccaactg ggcggagttc ctctggcgga ccaaccccgc gcagcccgac cgtcccgcgc
     5941 cgccgcaatc ccgtttcacc gagtggggcg aatggctgct gaccgtcacc gtcaccgagg
     6001 acccggccct gctggtcgtc gccgagtttt ccaaagatgt tacctggacc gaaccggagt
     6061 tcctcgctgg caactgggag acgaatggag acggcacccg caccggaacc ttccactgct
     6121 tcaattttgg cggctccgcc gggttcatcc gcttccgggg cgagtggtgc gggtctccgc
     6181 agccctgacg gccggcctgg cccggcgggg ttggttgccc gcgccgccgc gtccggatga
     6241 gcggctcggg tcaggcccag taacggtaat tgaggtcggg gaaaaggttg tcccgctcct
     6301 cgacctggcg gagccattcg cggtcgaggg gctcgccggc aagctgctcg taaaggcggg
     6361 tgaagcgcag gagatgctcc cgcacccgct tgcgggcgta gccggggcgg gtttcggatc
     6421 gcagcagaaa gggccaatcg ctggcctgcg ccagcagcag ctcgcgcgcg gcttgcttga
     6481 gcgcgcgctc ctgaatggcg tccggccgcg ggaaacgccg ggccagctcg gtcatccgct
     6541 cctgggcggc gcgcaggtgg ggatagatcc actcgttctt ctcgttgagc cagacgcgcc
     6601 aatagccgcc ctcgccccag cttgaggcgg cgggggtggc cgcctggtga gtgggatgcc
     6661 ggcgcaggaa ctcgcccggg gtgatgaagg tgaacgcccc gggctcggcg ccggcctggc
     6721 gcaccagcgc atccaggaac tcgggcccct caaaccacca gtggccgaac aactcggcat
     6781 cgtagggggc caccacgatg gccgggcggt cgagcgcttc gcccaggcgc tggaattgcg
     6841 cccgccgggc ggcgaggaag tggcgcgcgt gctcgccggc cgcgtggacg gcggcggcgc
     6901 gatcgtagat ttccttgtgg ggcgcgcggc cggtgatgcg atggtatttc aggccggtaa
     6961 agccgcggtg gccgggcgcg gccaggtgcg gggcgacgta ttccaggtcc aggtcaaacc
     7021 cgatgtcgcg gtagaaatcg cggtagcgcg ggtcgccggg gtagccggcc tggcggctcc
     7081 agacctggcg cgagcactcc ggatcgcggc caaacgcggc gatgcctttc ggggtaaaga
     7141 tgggcgcaaa caccgcgtag cgcgggcgcg ggcgcgcgtg caaaaggccg tgcgtgtcgg
     7201 tgacgaacca ccggaggtcc gcagcctgca ggacctcctc cagggcctcg acgtaagcgc
     7261 attccggcag ccagatgccc cgcggctcgc agccaaagca atgctggtaa tggtcgcacg
     7321 ccacccgcac ctgcgcccgc agggaaggcg ggtgatccgc cagcagcggc aacagggcgt
     7381 gcgtggcggc gctggtgatg atttccaggt ggcccgattc ctggaactgc cggaaggccc
     7441 gcaccaggtc gcgcccgtgc ccggcgtaaa gggcctgcgc ggcggtaaag cgttcgtgat
     7501 aaaaccgcgc cacctgctgg agcggctttt cccagcgcgt gcgctgaatc tccttttccg
     7561 ccagggcaac ctgctcccgg aggtagcgct cgcagcgatc ctgcaaaagc gggtcctgca
     7621 gcatcgcgca gagcgtgggc gtcagcgtca gcgtcaggcg cggggacagc ccatcccgcc
     7681 gccagccctc caaaagccgc agcagcggga gataggtttc cgcgacggcc tcgaacagcc
     7741 agttttcctc caggaactgc gcgtgctccg ggtgccggac gaacggcagg tgcgcatgca
     7801 ggatcagggc aaagtagccg ggcataaaga aacgcctttc cgccgccggc cggccgccgt
     7861 tcgcccgccc gcgccgttca tcacggttcc tcccccggcc gggacgccgg ttcagaggcg
     7921 tccgccccgc tcccgtccgg gtaatcggtg gcgcgggtga cccgcagccg ggcctgccgc
     7981 aaatcgcccc gggccgacag ggccgccacg cttgccgagt gctcgccgtc gggcagcgac
     8041 agccggcagc taaaggtgcc gtccgcccgg agggggattg gccgcccgcc aatcgtcacg
     8101 gtcgcatccg gctccgtcgc gccgtggatg acgaggtcgg tatggacgcg gaaccaaaag
     8161 ccaggcgccg gcggctccgc cgcgcccgct gcggggctgg agacgctccc ggcttccccg
     8221 cccgcaaccg cccaaagggc ggcttctccc tccgcccagg agcttagcgc gtctcccaat
     8281 tcgggagtcc atgccagcat ctccgccggc gcgtcttccc gcgcagtctc cggcggcggc
     8341 tcttttccgg gcgccgccgg gcgggctgga gttgccagtt gcgtcagccg cacctgggga
     8401 gggatcgtca cgaaccgcac ggtccgatcc gccgaaaccg tatccaccgg cgtggtcacc
     8461 ggggcggagg tggcgatttg cacccagcgg cgccgaggcc ggtagtagcc caactccgcc
     8521 acgtattgcg cgccggcgtg gctcacctgg atgaaccaat gccgtgattc cggatgcaca
     8581 tgaacttccg ccgccgggcg ctccgcgatt gaaccggcat gcacccgcac gacgagatgg
     8641 ccatccgccg accgggcgtt gtattggcgc tgctgccgcg cggccaaatc ccaggaagca
     8701 tacagccagc gcggctcgcg cgcggtgagc accagccggc ccgtcccata ggagccgggc
     8761 agggccgcgc ccctggactc aagcgggtcg cccgcggcgg ccggctccgg cccgggcgtt
     8821 ggctccgccc cgcgcaacgg cggacagggc ggctcgtctt cttccagcaa aagcggcggg
     8881 attagcgggt ccggcgccgc cggggagggc agcaccgcgg gcgccgctgg gcgcggcggg
     8941 cgcgcggcgg gcatccgctc gggcccgggc gcggcggcgc gtctggagac cgtccgcgcg
     9001 ccggttcgcg gagcgcgcgc gcggcgcggc gcgggcgtgc gcgggcggcg gcgcaccgtc
     9061 ttcttcactt tctccgccgg gctggcggcc ggggcggcct tgcgccgggc caccggggcc
     9121 ggcttctttg gacggcgagc acgggcgggc tttcccctgg ccgggcgggc cgggcggcga
     9181 agcggcgact tcttcggccg ggccgcgcgg cgcacggttc gccgggccgc cggggcggcg
     9241 gttgcaaggc gcggtttctt cttcttcata ccaggcacta ccccaggcgt cggggttgga
     9301 atctttctga tgcggtgaat cctacggcgg cggcccgccc ggcgcaatgc gaatctccgg
     9361 gcgcccgttt aatttgtgga aaaggagctt gcattcccgg cgggcattcg tagatttacc
     9421 cgcatagaac aggaagcgag cgtagctcag tctggtagag cgtcacgttg ccaacgtgaa
     9481 tgtcgagggt tcgaatccct tcgctcgctc cattttattt tccggaaccc gcctccgcaa
     9541 agaatcgaag agccgccgcg cgcgcctgat gaataaactg cgttcgaacc tggagcagaa
     9601 cctgggcggg gtggcgcttt tagtcctggg aatcggctgc ctggttgtct tgcggccgtt
     9661 tgtttccgcc ttgctgtggg cggtggtgct ctgcttttcg agttggcccc tgtatcagcg
     9721 cctgctccgc ctgctgggcc accgggcgac cctggccgcc accctgatga ccctggtcat
     9781 ggtgctggtg ttgttgctgc cgtttgtcct catcggcacc acgctgggca acagcatcaa
     9841 agacctgacc ctggccaccc gcaactggat ccaggacggg ccgcctcatc cgccggcgtg
     9901 gctcgcccgg gtgcccgtcg tcggcgcaac ggcggtggac tactggcaaa gcctggccca
     9961 ggacacggcc cggctatggg cgcaggcgca gcggttcatt gagccggtca gcgcctggct
    10021 gatccgggcc ggcctggccc tcgggggcgg cctgctggaa ttggccctga gcatcttcat
    10081 cgccttcttt ctgttccggg acggcgtctc cctggcccgg cggctggcgg cggccatcgg
    10141 gcgcatcggg ggcgagcgcg gttattacct gctgggagtg gccggccaga cagtgcgcgg
    10201 ggttgtctat ggcatcctgg gcaccgcgct ggcccaggcg attatggcgg gcctcgggta
    10261 cctcctggcg ggcttgcccg gcccgggcct gctggccctg ctgacgttct tcctctccgt
    10321 cgtgccggtg ctgggcacgt ccctggtctg ggggccggcg gcgttctggc tgttcaacca
    10381 gggctccacc ggctgggcca tcttcatgat catctggggc cttggcgttg ccagcctcga
    10441 caacttcatc aagccctggc tcatcagcca gggcagcaac ctgccgttca tcctgatctt
    10501 cttcggcgtg ctgggcggcg ccctggcctt tgggttcatc ggcgtcttcc tgggccccac
    10561 gctgctggcc gtgggatatc gcctggtgga ggaatggtcc gccggcgcgc gggaaacagc
    10621 ggccggcggc gaaacgccgc gcccggaata atctccaccc tccctgctcc cgcgtccggc
    10681 tccgggctaa tcctcggcgc gctcgcccgg aggggacatc ttcacaagct tcggatcaaa
    10741 ctggcccagg acggatacca ggtcgctctg ggcggccatg acgttgtgga tgttcttata
    10801 caccatcggc acttcatcca gccccgccga aagcagggtc accccccgcg cgcgcaggta
    10861 atgcttcacc gttttccagt cgaaagtccg gtgggcctgc gtgcggctca tgacgcggcc
    10921 cgcgccgtgc gccgccgagt tgagggattc ggcgttgccc ttgccgctca ccacaaagcc
    10981 cggcgtggcc atcgagccgg gaatgatgcc cagcacgccc cgccccgcgg gcgtcgcgcc
    11041 cttgcgatgc accaccacct cgcgctcgac gccgtccacc cggtgccgct ctttccaggc
    11101 aaaattgtgg tggttctcca agtccagccg cacctgcgcg cccaggtggg cggcgatatg
    11161 ccggtgaatc aacgcgtggt tggccgccgc gtagcgcccc atcaactcca tcgccgccca
    11221 atactcctgc cccgccgccg tgtccagcgg cagccaggcc agccgcttta attcgcccgg
    11281 caggcgcggt agctggcggg ccgcgagccg gctgtaataa tcacacaccg ccgcgcccgt
    11341 gccgcggctg ccgctgtggc tgagcagggc cacatacgtg cccggctcaa gcgactgaat
    11401 cttttcgtgc gcggtgaacc gcccgaactc gacaaagtgg ttcccgctgc cgctggtgcc
    11461 caactgcgcc caggcccggt ccttgttctc ccgggtgacg gggctgaccg accagtcggc
    11521 gtccatcacc tcatgccacc ggcgcggctt gaagttcgac cccacgccaa accgcgtttc
    11581 tgccgcgagg gcgcgcgcca gccgctccag gggccgatcc agctcgcgca gcggaaggtc
    11641 cagcaccgtc aacttcatcc ggcaggcaat atccactccc accgcgtacg gaatgactgc
    11701 gttctccgtc gccagcaccc cgccaatcgg cagcccgtaa cccacatgcg catccggcat
    11761 cagcgccccc gccaccgcca ccggcaactg gcacgcccgt tccatctgcc ggaccgcctc
    11821 cgcctccagc ccttcgcccc actggcgata ccgggccggc gccgcgcgcg gcggcggcgc
    11881 cgactgcagg gcccgggcaa acgccccgcg cagcgggtcc gccgtgaaac tgcccggatt
    11941 ggccacaatc gccgccactt cctccgtcag ccgggcccga tccccgccgc cgaggataaa
    12001 ccggctgata aaatccgtcg cccgccgggt tgcttcccct ggcggcactc cgagccgaat
    12061 caagtctttg gcattcaccg aatcatagcc agtttaaacc gaaacagccc caaatgaaat
    12121 aacaatgcct cgggcaacga gccatttcca ggggctttgg ttttatagat catgagccgg
    12181 ggaggctgaa gctgcggttt gggcggcggt tatgcgcttc ggagcggatg tctggccgcg
    12241 ctgcccgggg cttgagccat tgggaacagg ggaggtgaga tcgccctcca aggcgagcgg
    12301 gttttcctgc aggcaatcca ggcggacgcg cagatgtttt tctcagtcgc cggacgaaag
    12361 acccccgagc gccgcgtgag ggcacgcggc ctacaggaaa aggaagcacg ctgcggcaaa
    12421 ccccatgccc tctcgcagca ccgcactccc gacaacccac aacccccccc gcaccctccc
    12481 gccccctgta ggccgcgtgc ccacacgcgg cgctttgcac ccgcctgcca ccaccacccc
    12541 cccaaaattg cccttgcgcg aagcctcccc gcccgccagc cttcccgtat tacctcgggg
    12601 cagacttcca gcccaaggag tggcgggaag ccgaaccgcc tccgggccgt aatagaacgg
    12661 caattaaaac aacaattaga gaggacagac attatgaaaa tactatctgc tccgcgcacg
    12721 ggcagtctgg gcgcggaaac
//
LOCUS       DAOPQG010000099        12441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5899_length_12441_cov_15.596884, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000099 DAOPQG010000000
VERSION     DAOPQG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12441
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_12441_cov_15.596884"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..1401
                     /locus_tag="PKI07_10750"
     CDS             544..1401
                     /locus_tag="PKI07_10750"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOC51568.1"
                     /translation="MHKRIYSTLLALSLAAVPALAQFEGMLEMKITTTGGDRELGGNG
                     KMMTAVGKAGSRCEMNLEMGGMAMKMVILQKTDTPDLVYHINDANKTYTEIDLAKMRK
                     MAEQTADARQYTVQKLGQETILGYKTQHVLVTEKDPGHSKGMTTELWTAKGLLDYTTF
                     ARMQPRPGKKGGEEALTKALKDADADGLPLKAINTSPDGTKITMEVVKIDKQSLPAST
                     FQIPAGYTKSTGGLLDMMGGLSGPSTGEAKQQLNQVQQEMQEALKNMPPEQRAMIEKM
                     MKQQAAPNP"
     gene            1625..1948
                     /locus_tag="PKI07_10755"
     CDS             1625..1948
                     /locus_tag="PKI07_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HOC51569.1"
                     /translation="MKKLIGYSALVAVAGLLVGCVGPMGPAGSVGGSIYTDVSGPVLA
                     TSHSTASKVGTASSTGVLGFAWGDSSIKAAASNAGITKIQHVDFKTTSVLGIYAKTTV
                     VVYGE"
     gene            complement(2058..3185)
                     /locus_tag="PKI07_10760"
     CDS             complement(2058..3185)
                     /locus_tag="PKI07_10760"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOC51570.1"
                     /translation="MRLLDRYLLRELLIPLGYCLGGFLLFWITSDLFVTLEDFRKKAL
                     GARDIVDYYLAVLPEFLVLVLPIALLLALLYALTNHARHQEITAIRAAGISLWRLALP
                     YFGVGLLLSGILFFVNEWLVPPGEEKAQEILKRHQPFDPNAPGRNEHRNLYFENTRDG
                     RRWRIGVYHSETGEMSGLGVYPTESGGARPWELRAERARRIEGVWTFYNVLVLQETAD
                     PTAPPVPTLRTNLLAVPEFQETLEEINSEIKIRESMTLRAAKKADVPIRDLLNYLRLH
                     PRLSRADWYWLYTKLDGRLAAPWTCVVVVLVAVPFGVVGGRRNVYVGVASSILICFSY
                     FVLQQIGLALGTSGRIEPWLAAWFPNLFFSLIGFWLAIRVR"
     gene            3301..5670
                     /locus_tag="PKI07_10765"
     CDS             3301..5670
                     /locus_tag="PKI07_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOC51571.1"
                     /translation="MKKILIHLLWVLLALAGAWAYATLALHRGEHLNSAYLLIAALCT
                     YAIGYRFYSKWLAARVMMLDDRRATPCEVHDDGKDFVKTNKWIVFGHHFAAISGPGPL
                     VGPVLAAQFGYLPGTLWILIGVVLGGAVQDFVILFCSIRRDGKSLAQMIRDELGTAAG
                     TVAAVAIVAILIILLAVLALVVVNALGESPWGVFTVGATIPIAMFMGGYLRFWRVGRI
                     REVTVIGVVLLLLAVWGGQYVYHHPGLARFFSLGKEPIAWSIILYGLAASVLPVWLLL
                     APRDYLSTFMKLGTIFALAGGVLVVLPNLNMPAFTRFVEGTGPVVPGGVFPFCFITIA
                     CGAISGFHTLIASGITPKIITRESYARLVGYGAMCLESLVAIMAMIAACTLQPGVYFS
                     MNVDVGDLPVAAKVAATAAKITSEGFPVTAERMANLAAAVGEPSLVGKTGGAVTLAVG
                     MAEIFSRVTQGRWLDLWYHFAIMFEALFILTTIDAGTRVGRYLLQDAFGHIWKPLRQT
                     GHFGANLLASGSMVLGWGFFLIMGVRDPEGGVKALWPIFGISNQMLAGIALCLATTVV
                     LKMALKQGESALRAVALALTTLVPLVWLLGVTFTAGAQKIGHAKPGIGFLAANRVLQE
                     QVPKLQAALAAAETGGDPAALAAAQKALRFNRVKWFNNTVDASATAFFMALVLIIVVL
                     SVRDWIQLLRRQKPAVLSETEPVWLPAYAVEEARPLSAVGAGALALALTKELSGEADL
                     ERARHCEPACEVWAAKCPASADRLQPGKAAGQIYAELTEQRYRGVRRCC"
     gene            5693..6268
                     /gene="nadD"
                     /locus_tag="PKI07_10770"
     CDS             5693..6268
                     /gene="nadD"
                     /locus_tag="PKI07_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOC51572.1"
                     /translation="MQRIGLYGGSFDPVHRGHLLVAQAAREELALERLFFIPAARSPF
                     KQEVWPSPAPERLRLLRLALAGQAESEIDEQEIQRGGISYTIDTVRDYARRFPGARLF
                     WLIGADHLPQLPEWRAAEELAQRVEMVVIPRPGQTVQPFPAPFRGHWLEGFPLGVSSS
                     RIRARVKAGLPLTHLVPDSVAEAIRNNRLYL"
     gene            6295..6672
                     /gene="rsfS"
                     /locus_tag="PKI07_10775"
     CDS             6295..6672
                     /gene="rsfS"
                     /locus_tag="PKI07_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOC51573.1"
                     /translation="MDARKLALLCRELADNKKAENIIILDVRELSSVTDYFVIATGTS
                     EPHLRAIMDEISDRLREDHGLRPHAIDGTIRTAWVVLDYFDVIVHLMRHDVRAHYDLE
                     TLWGDAPRLRPRRRAASKAPPPG"
     gene            complement(6685..6984)
                     /locus_tag="PKI07_10780"
     CDS             complement(6685..6984)
                     /locus_tag="PKI07_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PUR family DNA/RNA-binding protein"
                     /protein_id="HOC51574.1"
                     /translation="MISNDPKSLSGNHPYEERAPRQPRAPVNEDTLKSVKIQVERKIF
                     VLALKENVRGRFLRITEDVGGRRDTIIIPAPGLAEFRQVLDEIIKLSDETPPKAA"
     gene            complement(7090..7629)
                     /locus_tag="PKI07_10785"
     CDS             complement(7090..7629)
                     /locus_tag="PKI07_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HOC51575.1"
                     /translation="MIGFLRFVGILNVGVWLGAAIFFTFGVGLAAFSPEMQNLLGANN
                     YPCYSGAIAQVFAARYFHFQKICAAVAVVHLLAEWLYLGKRPRKFQSGVLLGLCAAVL
                     LGAGWLQPKLKALHAAQYSPQARPETRATASRSFKAWQGVSQGVNVLVIAGLGVYLWR
                     AANPSDPTRFVSAMKFALR"
     gene            8023..8430
                     /locus_tag="PKI07_10790"
     CDS             8023..8430
                     /locus_tag="PKI07_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC51576.1"
                     /translation="MDEANTKKTETTQRGRALIYVVDDEPMLLDLAAVILRTLGYTVA
                     TFRAPEATLQAYRAAAMPPALLITDYAMGAMNGLELAAACRKICPQQKVLLLSGTVGV
                     ADLQGGPTWPDQFLAKPYQPKQLVNAVESLLAA"
     gene            8504..9277
                     /locus_tag="PKI07_10795"
     CDS             8504..9277
                     /locus_tag="PKI07_10795"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOC51577.1"
                     /translation="MKKLIIRLVIALVVVAGLAILAVSLFLGTAVKRGVETLGPKLTK
                     VDVKLQTVSLSPLSGAGSIKGLVVGNPEGFKSPAAISIGEASLSLKPASLLADKVIIK
                     SIRIQGPEITYETNLKQNNLSQIKANLDAALGGGPSEPASPKEAKPPRKLQVDEFFLT
                     GGKIHVRANTPLGSGTASVPLPDIQLQDLGAGPDGITAAELAAKVLSAIEESALKASS
                     DALADIGKGAVYITKDPASAASNVLQKATKSVGDLFKKK"
     gene            complement(9375..10559)
                     /locus_tag="PKI07_10800"
     CDS             complement(9375..10559)
                     /locus_tag="PKI07_10800"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOC51578.1"
                     /translation="MQSLPCISLAWLALAGGLVYASAVSSETPAVDEAMLVYIGTYTG
                     ARSKGIYVSRFHPLSGELTAPELAAATPNPSFLALHPNQRYLYAVGETAESPGARTGT
                     VCAFRINAADGQLALLNRESSGGAGPCHLAVAGAGKCLLVANYGSGSVAALPLQATGE
                     LGKPGTVIQHRGSSVNPQRQAGPHAHFITAAPDDRFALACDLGLDQVLVYRLDPAKAG
                     LAANDPPFAAVKPGAGPRHLAFHPSGRFVFLINEMDSTLAVFAYDARRGALEHVQTVS
                     TLPEAFTGQSTCAGILVHPSGRFVYGSNRGADSIAVFSFDARQGRLKLVEHRPTQGKT
                     PRHIALDPTGQWLLATNQGSDQVVVFRVDLKAGRLRGGGKPATVGAPVCAVFVPGKPP
                     QP"
     gene            complement(10654..11565)
                     /gene="hypB"
                     /locus_tag="PKI07_10805"
     CDS             complement(10654..11565)
                     /gene="hypB"
                     /locus_tag="PKI07_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOC51579.1"
                     /translation="MCNDCGCGLPGEKPAGVGAHHSEPAHEHGHGHEHEHDHEHGPGH
                     HHGHDHSHPHRHPHTHAEAHAEPGHDAAPPAENEARRTVEVRRAILEKNERLAERNRG
                     FFRARGLLVLNFLSSPGSGKTTFIRESVRRLEPDLKAGVIVGDLATDNDARRLRESGA
                     PVVQVTTGTVCHLEAEMVGRAVQQLDLRDRNVLIIENVGNLVCPASYDLGEDLRVVLL
                     SVTEGEDKPLKYPPMFQSADVVIISKIDLAAACGYDRAAALANLRRVSPRARIFETSA
                     RTGEGMDAWREFLCEQQRARRTLGPLG"
     gene            complement(11565..11906)
                     /gene="hypA"
                     /locus_tag="PKI07_10810"
     CDS             complement(11565..11906)
                     /gene="hypA"
                     /locus_tag="PKI07_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOC51580.1"
                     /translation="MHELSIMQSALGLALKQTREAGGGRVHVIRLRIGVLSGVVPDAL
                     EFAFGALAPGTPAEGARLVIESVPARFWCEACAREFQSEDLLAECPDCHRASGELRAG
                     REMELASLEID"
BASE COUNT         2426 a         3966 c         3865 g         2184 t
ORIGIN      
        1 ggtctggata atggatgtgg ataccaggaa aggccgcaac gatgagcggg aacgggagga
       61 taattactaa gttcagtaaa aacaggcagt tggaaatctg aggttgacgg aagggtagta
      121 ttcaaaggag cgcaagcacc cggatggctt ttggtttcgc gattggcgca tctgaaacgt
      181 tgacgtggca taatccagcc agacgccttc gttccactgg atccgaagcg ggcctgacag
      241 gcctgcggaa agcctcaacg aactgttgga ttggtgaatt caatgggcgg gtcgctggat
      301 cccgaatggc tttcacccgt aaaaccaggg acttgagcgc tacgtggggt cgaatgggag
      361 gcgaaatcgg aatcggtccg gctgaattat cctgaggctg caagaccgcc acgggggcgt
      421 tttcgctgaa ccgggtccgg ggcatgcctc aaggacggat tcaaagtaga cctcccgtgg
      481 tttgacaacc ctgccttgac gtgctacccg tgggaaaaac tggtaactgt ttaaggtaat
      541 tttatgcata aacgaatcta ttctaccctg ctggcgctct cattagcggc tgttccggca
      601 ctggcccagt tcgagggcat gctggaaatg aaaataacca ccacgggcgg ggatcgcgag
      661 ttggggggca atggcaagat gatgaccgca gtcggcaaag ccggttctcg ctgtgaaatg
      721 aacctggaga tgggggggat ggccatgaaa atggtgatac tgcagaaaac ggatacccca
      781 gatctggtgt accatattaa cgacgccaat aagacgtaca cggagattga cctcgctaag
      841 atgcgaaaaa tggcggagca gacggctgac gcccgccaat acaccgtcca gaaactcggc
      901 caggagacca ttctcggcta caaaacccag catgtgctgg tgacggagaa agatcccggg
      961 cattccaagg gcatgacgac tgaactgtgg acggccaaag gactgctgga ttacacgacc
     1021 tttgctcgaa tgcagccccg gcccggcaaa aagggcggtg aggaagctct gaccaaagcg
     1081 ttgaaagatg ccgacgccga tggcctgccg ctcaaagcca tcaacacttc tccggacggc
     1141 acgaaaatca ccatggaagt ggtaaaaatt gacaagcagt cactcccggc ttccactttc
     1201 caaatccccg ctggttatac caaatccacg ggcggcctgc tggacatgat gggcggcttg
     1261 tccgggccgt caaccggcga ggccaaacaa cagctcaacc aggttcagca ggaaatgcag
     1321 gaagcgctga aaaacatgcc gccggagcag cgcgcgatga tcgaaaagat gatgaagcaa
     1381 caggcggccc ccaatccgta aatcgcccgc gcgccgccgg gggcagctaa agaacgcata
     1441 tcggcgccgg gcggcggagg aatagtcaaa atctcctgca tcgcattcgc ccagcgcgga
     1501 tgtgcgccag cggatgccgg gagttggatt tgacaagcag cggcggatga tttagagagg
     1561 ctttattcca gacggcagta attgagggcc gcctggcaca caaacaaact aacaacaaaa
     1621 gcatatgaag aaactaattg gttattccgc actggttgcg gtggcaggac tgctggtcgg
     1681 ttgcgtaggc cctatgggcc cggccggaag cgtgggtggc agtatttaca ccgacgtttc
     1741 gggtccggtc ttggccacca gccattccac cgccagcaag gttggcacgg cttcctccac
     1801 gggtgtgttg ggctttgcct ggggtgattc cagcatcaag gcggccgcca gcaatgccgg
     1861 catcaccaag attcaacacg tggacttcaa gaccacctcg gtgctgggca tctacgccaa
     1921 gacgacggtc gttgtttacg gcgaataaac ccgccttttt ctcaaacaga caagaccggt
     1981 tcccttccgg aaccggtctt tgcttgtcac ggccagcccc gcaacgggcg ccccgccttc
     2041 ccgccgcggc cgggcggtca tcggacccgg atggccagcc agaacccgat caggctgaag
     2101 aacaggttgg ggaaccatgc cgccagccag ggctcgattc gcccgctggt tccgagcgcc
     2161 agcccgatct gctgcaggac gaaataggaa aagcaaatga ggatgctgct ggccaccccc
     2221 acatacacgt tgcgccgccc gccgaccacc ccaaagggga cggccaccag caccaccacc
     2281 acacacgtcc acggcgcggc caggcgtccg tccagttttg tgtacagcca ataccagtcg
     2341 gcgcgcgaga gacgggggtg caaccgcagg tagttgagga ggtcccggat ggggacatcc
     2401 gccttcttgg cggcgcgcag cgtcatgctc tcgcggattt tgatctcgct gttgatttct
     2461 tcgagtgttt cctgaaactc cgggacggcg agcaggttgg tccgcagcgt tggcaccggc
     2521 ggcgcggtgg gatcggccgt ctcttgcagc acgagaacat tatagaaggt ccagaccccc
     2581 tcgatccggc gcgcccgttc ggctcgcagt tcccagggcc tggcgccgcc cgactcggtg
     2641 ggatacactc ccagcccaga catctcgccc gtctcggaat ggtaaacgcc gatgcgccag
     2701 cgccggccgt cgcgcgtgtt ctcgaaataa aggttgcggt gttcgttgcg gcccggggcg
     2761 ttggggtcga agggctgatg gcgcttgaga atctcctgcg ccttttcctc gcccggcggc
     2821 accagccatt cgttgacgaa gaacaggatt ccgctcagca gcaggcccac cccgaagtag
     2881 ggcagcgcca ggcgccataa gctgatgccg gcggcgcgga tggccgtgat ttcctgatgc
     2941 cgcgcgtgat tggtcagcgc atagagcagc gccagcagca gcgcgatcgg cagcaccagc
     3001 accaggaatt ccggcagcac ggccaggtag taatccacga tgtcgcgcgc gcccagggcc
     3061 ttcttccgga aatcctcgag ggtcacaaac aggtcggacg taatccagaa cagcaggaac
     3121 ccgcccaggc aatagcccag cgggatcagc agctcgcgca acagataccg gtccaacagc
     3181 cgcatttcgc cgcagcctag ccgccggtcc gccccaaagc aagcggcggc acccgccgcg
     3241 gcgggcggtg aatctaggct ttgcagccgc cgcgcaaagc aggtatgtag gaggcgcgga
     3301 atgaagaaaa tcctgatcca tctgctgtgg gtgctgctgg cgctggcggg agcctgggcc
     3361 tacgccacgc tggcgctgca ccgcggcgag catctcaact cggcctacct gctcatcgcg
     3421 gcgctgtgca cctacgcgat tgggtatcgg ttctactcca aatggctggc cgcgcgcgtg
     3481 atgatgctcg atgaccgccg ggccacgccc tgcgaagtgc atgacgacgg caaggatttc
     3541 gtgaagacga acaaatggat cgtcttcggg catcacttcg ccgccatctc cgggccgggg
     3601 ccgctggtcg ggccggtgtt ggcggcccag tttggttatt tgccgggcac gctgtggatc
     3661 ctgatcggcg tggtgctggg cggcgcggtg caggattttg tcatcctgtt ctgctcgatt
     3721 cggcgcgatg gcaaatcgct ggcgcaaatg atccgcgacg aactgggcac cgccgcgggc
     3781 acggtggccg cggtggccat cgtcgccatt ctcatcatcc tgctggcggt gctggccctg
     3841 gtcgtcgtca acgcgctggg ggagagtccg tggggggtgt tcacggtggg ggcgaccatc
     3901 ccgattgcga tgttcatggg cggctatctg cgtttctggc gcgtggggcg gattcgcgag
     3961 gtgaccgtga ttggcgtggt gctgctgctg ctggcggtct ggggcgggca atacgtctat
     4021 caccatccgg gcctggcccg gtttttctcc ctgggcaagg agccgatcgc gtggagcatc
     4081 attctctatg gcctggcggc gagcgtgctg ccggtgtggc tgctgctggc gccgcgggat
     4141 tatctcagca cgtttatgaa actgggcacg atcttcgcgc tggccggcgg ggtgctggtg
     4201 gtgttgccga acctgaacat gccggcgttc acccggtttg tggagggcac ggggccggtg
     4261 gtgccgggag gggtgttccc gttctgcttt atcacgattg cgtgcggggc gatttccggc
     4321 tttcacacgt tgatcgccag cggcatcact cccaagatca tcacccggga gagttatgcg
     4381 cgactggtgg gctacggcgc catgtgcctc gagtccctgg tcgccattat ggccatgatc
     4441 gcggcctgca ccctgcagcc gggggtgtat ttcagcatga atgtggacgt gggcgacctg
     4501 ccggtggcgg ccaaggtggc ggccacggcg gcgaagatca ccagcgaagg atttcccgtg
     4561 acggccgagc ggatggcgaa cctggcggcg gccgtgggcg agcccagcct ggtcggcaag
     4621 accgggggcg cggtgaccct ggccgtgggc atggcggaaa tcttctcacg ggtgacccag
     4681 gggcgctggc tcgatttatg gtaccacttc gcgatcatgt ttgaggcgct gttcatcctg
     4741 acgaccatag acgcgggcac gcgcgttggc cgctacctct tgcaggatgc cttcggtcat
     4801 atttggaaac cgctgcgcca gacgggccat ttcggcgcca acctgctggc cagcggcagc
     4861 atggtgctgg gctggggctt tttcctgatc atgggagtgc gcgacccgga gggcggggtc
     4921 aaggcgctct ggcctatttt cggcatctcc aaccagatgc ttgccggcat tgccctgtgc
     4981 ctggccacga ccgtggtttt gaagatggct ttgaagcagg gtgagtcggc gctgcgcgcg
     5041 gtggcgctgg cgctgaccac gctggtgccg ctggtctggc tgctgggcgt caccttcacc
     5101 gcgggcgcgc aaaagatcgg gcacgcgaag ccgggcattg gttttctggc cgccaaccgg
     5161 gttctgcagg agcaagttcc gaagctgcaa gcggccctgg cggccgctga aaccggcggc
     5221 gacccggccg cccttgccgc ggcgcaaaag gccttgcgct tcaaccgcgt caagtggttc
     5281 aacaataccg tggacgcttc ggccacggcc ttcttcatgg ccctggtgct gatcatcgtc
     5341 gtgctgagcg tgcgcgactg gatccagctc ttgcggcgtc aaaagccggc ggtgctgagc
     5401 gaaaccgagc cggtatggct gccggcgtat gcggtggagg aagcccgccc gctctccgcg
     5461 gttggggcgg gagccctggc cctggcgctg accaaggaat tgtcgggcga ggcggacctg
     5521 gaacgcgccc gccactgcga gccggcttgc gaagtgtggg cggcgaaatg tccggcgtcc
     5581 gccgaccgcc tccaacccgg caaggccgcg gggcaaatct atgccgagct cacggagcaa
     5641 cgataccgcg gcgtccggcg gtgctgttga agccgccggc gggagagaga tcatgcaacg
     5701 cattggctta tacggcggtt cgtttgaccc ggtgcaccgg ggccacttgc tggtggcgca
     5761 ggcggcgcgg gaggaactgg cgctggagcg cctgttcttc atcccggcgg cgcggtcgcc
     5821 gttcaaacag gaggtttggc caagcccggc cccggagcgg ctgcgcctgc tgcgactggc
     5881 cctggcgggg caggctgaga gtgaaattga cgagcaggaa atccaacgcg gcggcatctc
     5941 ctacaccatt gataccgtgc gcgattacgc gcgccggttc ccgggcgcgc ggcttttttg
     6001 gctgatcggc gccgaccacc tgccgcagtt gccggagtgg cgcgcggcgg aggaactggc
     6061 gcagcgggtc gaaatggtgg tcattccccg gcccgggcag accgtgcagc cattccccgc
     6121 gccttttcgc gggcattggc tggaaggctt cccgctgggc gtttcctcct cccggattcg
     6181 ggcgcgggtg aaggcgggcc tgccgctcac gcatctggtg ccggatagcg tggccgaggc
     6241 gatccgcaat aatcggcttt atctttgagg cccgcccggc gagaataacc ccagatggac
     6301 gcaagaaaac tggcgctgct ttgccgcgaa ctggccgaca ataagaaggc ggaaaacatc
     6361 atcatccttg atgtgcgcga gctgtcatcg gtcacggact atttcgtgat tgccaccggc
     6421 acgagcgaac ctcatctgcg ggcgattatg gatgaaatca gcgaccgcct gcgcgaggat
     6481 catggcctgc gccctcacgc cattgacggg acaatccgca cggcctgggt ggtgctggac
     6541 tacttcgacg tcatcgtgca cctgatgcgc cacgacgtac gcgcgcacta cgacctggaa
     6601 accctctggg gcgacgcgcc ccggctcaga cccagaagac gggcggcgtc caaagcgccg
     6661 ccgccgggtt gatcccggcg ggcgctacgc cgccttgggc ggcgtttcgt ccgaaagctt
     6721 gatgatttcg tccagcacct gccggaactc cgccaacccc ggcgcgggaa taataatcgt
     6781 atcacgccgg ccgccgacat cctcggtgat gcgcagaaag cgcccgcgca cattttcctt
     6841 gagggccagg acgaagattt tgcgctcgac ctgaatctta accgatttca gcgtgtcctc
     6901 attgaccggc gcccggggct ggcgaggagc ccgctcctcg taaggatgat tgccacttag
     6961 cgactttgga tcgttcgaga tcatatactt ttcccgttcc gggctccccg caggcagccc
     7021 cacttaattg gtttaactct aagaataacg tccgcgacca cgccagattg tcaacccccc
     7081 gccagttctt taccgcaacg caaacttcat ggcgctcacg aaacgagtcg ggtccgaggg
     7141 attagccgcg cgccataaat agacgcctag cccggcgatc accagcacat taaccccctg
     7201 ggacacgccc tgccaagcct taaaagaccg gctggcggtg gcgcgggttt ccggccgggc
     7261 ctgggggcta tattgcgcgg catggagcgc cttcaacttg ggctgaagcc atcccgctcc
     7321 cagcaggacc gcggcgcaca accccagcaa caccccggat tggaacttcc gcggacgctt
     7381 gcccaggtag agccactccg ccagcagatg caccaccgcc accgccgcgc agatcttctg
     7441 gaaatgaaaa tagcgcgcgg cgaaaacctg cgcgatggcc ccggagtaac aaggatagtt
     7501 attcgctccc agcagattct gcatctcggg cgaaaaagcg gccagcccca cgccgaacgt
     7561 gaagaaaatc gcggccccca gccacactcc cacattcaga atgcccacga atctcaagaa
     7621 cccaatcaca acgctaccct aagagaccgt gcccgccctg ccaagctttt tgacacccac
     7681 ggccgcagca tccgcgcgcg cagccattcg cagccgccaa acggctgccg cgctgcttcc
     7741 cgacaacacc ccgtccaccc cgccgacacc cacattctcc acctcatccg attggcgttt
     7801 tccttctatc ccccaaccgc tgcttctcta actccaggct ggaaatgcca tgaccttgga
     7861 tgccaagcgt cgggagggtt aataaagaat gatgacggcc aaacctgaat cggactggca
     7921 atccgccgca aagaccgcgc tgcgccccgg tttcccgaaa accgcgaata ttacttgctt
     7981 attatccgga actggcgttt attagggata ggtatttata atatggacga ggcgaacaca
     8041 aagaaaacag aaaccacgca gcggggccgg gcgctcattt atgttgtgga cgacgagccc
     8101 atgctgctgg atttggccgc agtcattctg cgaacgttgg ggtacaccgt tgcgactttc
     8161 cgcgccccgg aagcaaccct gcaggcctac cgcgccgccg ccatgccgcc ggccttgctc
     8221 attaccgatt atgcgatggg cgcaatgaat ggcctggagt tggctgccgc ctgccggaag
     8281 atttgcccgc agcagaaggt gcttttgctc agcggcacgg tgggggtcgc ggaccttcag
     8341 ggcgggccaa cctggccgga tcaatttctg gccaagccgt atcagcccaa acaactggtt
     8401 aacgccgtcg aatcgctgct ggcggcgtag cggtcttccc gcagatgctc cggccttata
     8461 gaacaccagc agcggcgcgg catcgcaagc gcactaacga attatgaaaa agttgatcat
     8521 acgcctggtt atcgccctgg tggtggtggc gggactcgca atcctggcgg tcagcctgtt
     8581 cctgggcacc gccgtcaaac gcggggtgga aaccctggga ccgaagctaa ccaaagtgga
     8641 cgtcaaactc caaacggtca gcctgtcccc gctctccggg gcgggttcga ttaagggcct
     8701 tgtggtgggc aaccccgagg gcttcaaatc gccggccgcc atctccatcg gtgaggcgag
     8761 tctatcgctc aaacccgcct cgctcctggc ggacaaagtc atcatcaagt ccatccggat
     8821 tcagggcccg gagatcacct acgaaaccaa cttgaagcag aacaatctga gccagatcaa
     8881 ggccaacctg gacgcggccc tgggaggcgg accgtccgag ccggcttcgc ccaaagaagc
     8941 caagccgccc cggaaactcc aggtggatga gttcttcctc accggcggca agattcatgt
     9001 ccgcgccaac acccccctgg gcagcggcac ggcctcggtc ccgttgccag acatccagtt
     9061 gcaggacttg ggcgcgggcc ccgacggcat cacggcggcg gagttggcgg ccaaggtgct
     9121 ctccgccatc gaagaaagcg ccctcaaagc ctcctccgac gcgctggccg atattggcaa
     9181 aggggcggtt tacatcacga aagatccagc cagcgccgcc agcaacgttc ttcaaaaagc
     9241 caccaagtcc gtcggtgact tgttcaaaaa gaagtagcgc ccccgcttcc ctgccaccca
     9301 tgcccgggtt gcgccgggag cacccgccgg gcagcaactt gccgcggaat cacgcgcccg
     9361 cgggagggag ccgttcaggg ttgcggcggc ttccccggca caaatacagc gcatacgggc
     9421 gcgccgacgg tcgccggctt ccccccgcca cgcagacggc cagccttgag gtcaacccgg
     9481 aagaccacga cctggtctga tccctggttc gtcgccagca accactggcc ggtcgggtcc
     9541 agggcaatat gccggggagt cttgccctgg gtggggcggt gttccaccag cttgagccgg
     9601 ccttgccggg cgtcaaagct aaacaccgca atgctgtcag cgccgcggtt cgagccataa
     9661 acaaaccttc cagaaggatg caccaggatt cccgcgcaag tgctctggcc cgtgaacgcc
     9721 tccggcaatg ttgaaacagt ctgcacatgc tccaacgcgc cgcggcgcgc gtcgtaggca
     9781 aacaccgcca gcgtggaatc catctcgttg atcaaaaaga caaagcggcc cgaagggtga
     9841 aacgccaggt ggcgcgggcc ggcgcccggc ttgaccgcgg cgaacggagg atcattcgcc
     9901 gccagcccgg ctttggccgg gtccagccga taaaccagca cctggtccag gccgagatcg
     9961 cacgccaggg caaaacggtc gtccggagcc gccgtaataa agtgagcatg cgggccggcc
    10021 tgccgctgcg ggttcacgct ggaaccccgg tgctggataa ccgtgccggg tttgcccaac
    10081 tcgcccgtgg cttgcagcgg cagggccgcg acgctcccgc tgccgtaatt ggccaccagc
    10141 aggcatttcc cggcgcccgc caccgccagg tggcaaggcc ccgccccgcc ggaagattcc
    10201 cggttgagca aggcaagctg gccgtccgca gcgttaatcc tgaacgcgca aaccgtgccg
    10261 gtgcgcgccc cgggcgactc ggccgtttcc cccaccgcat aaagataccg ctggttggga
    10321 tggagggcca gaaaactggg attgggtgtg gccgccgcca gttccggcgc ggtcaactcg
    10381 ccggacaggg gatggaaccg ggaaacgtaa atgcctttgc tgcgggcgcc ggtataggtg
    10441 ccgatataaa ccagcatggc ttcgtcgacc gccggcgttt ccgaggacac ggcggaagca
    10501 taaacaagcc cgccggccag cgccagccag gcaaggctaa tgcagggcaa cgattgcatg
    10561 gggccagaaa gcatgatccg ccgccgtttt caaccccgaa acgcgggaaa gtccgccgcc
    10621 gggcgtctcc ggatggccaa ccgcgcccgg ccgttaaccc aggggcccaa gtgttcggcg
    10681 cgcccgttgc tgctcgcaca gaaactcgcg ccaggcatcc atcccctctc ccgtccgcgc
    10741 cgacgtctca aatatccgcg cccgaggaga cacccgccgg aggttggcca acgccgcggc
    10801 ccgatcgtaa ccgcaggccg ccgccaggtc tatcttgctg atgatgacca cgtccgcgga
    10861 ctgaaacatc ggcggatatt tcaacggctt gtcctcgccc tcagtgacgg acaacagcac
    10921 aacccgcaaa tcctcgccca ggtcgtagga agccgggcag accaggttgc cgacattctc
    10981 gatgatgagc acgttccggt cgcgcagatc aagctgctgc acggcgcggc ccaccatttc
    11041 cgcttccaga tggcagaccg tgccggtggt gacctgcacc accggcgccc cggattcgcg
    11101 caagcgccgc gcgtcattat ccgtcgccag gtcgccgaca atcacccccg ccttgagatc
    11161 cggctcgagc cggcgcacgc tctcgcggat aaaggtcgtc ttgcccgacc cgggcgagga
    11221 aaggaagttc aacaccaaca ggccgcgcgc ccggaagaaa ccccggttgc gctcggccag
    11281 ccgctcgttc ttctccaaaa tggcgcgccg cacctcgacg gtccgccgcg cctcgttttc
    11341 ggccgggggc gcggcgtcgt gacccggctc cgcgtgcgcc tcggcatggg tatgcggatg
    11401 ccggtgcggg tgggaatggt cgtgcccgtg gtgatggccc ggcccgtgtt catggtcatg
    11461 ctcatgctca tggccgtgcc catgttcatg agccggctcc gaatgatgag cgccaacgcc
    11521 cgcgggcttt tccccgggca aaccgcatcc acaatcatta cacatcagtc aatctccaaa
    11581 gaagccaatt ccatttcccg gccggcgcgc aactctccgc tggcgcggtg gcaatcggga
    11641 cattccgcca gcagatcctc ggactggaac tcccgcgcac acgcttcaca ccagaaccgc
    11701 gccggcacgg actctatgac caggcgcgcg ccctcggccg gggttcccgg cgccaaggcc
    11761 ccaaacgcaa actccagcgc atccggcacc accccgctca acacccctat gcgcaggcga
    11821 atcacatgca cacgcccgcc cccggcctcc cgcgtctgct tcagcgcgag gcccagcgcg
    11881 ctttgcataa tagacaactc atgcacgggg tcgttctggc aagatatttc gctactccct
    11941 ttctgacaac tggatttcgc agcgacgcaa gccaggacgc tcctctgttt atcagcggct
    12001 ttcccggttt tgcgaacggc ggccgcatag ccgggccggg gagggctgtt aaagctggac
    12061 cgctgcacgg gggcagggag tgccgccgcc ttgccattat cggtcggccc cggtgaaacc
    12121 tcttcctctc gctgctgtag cactatttga cctaaccaaa acgactcagg tttcagcttc
    12181 aatttgaccc cacgaaacgc gcaagtccct gattttacgg gcgaaaggca ttcgcaagcc
    12241 ggttgttagc ccgttgaaat cgccagccaa acggttcatt gaggcccccc gccggcctgt
    12301 caggcccgct ttgaccccaa cggagcgagc cagcacggtc gaaccgctca acgtcaacgt
    12361 ttccgataat acatgggcga agcccaaagc cattccggca ctcgcccgcc cttaaaaaac
    12421 tcacttccgc ccgcctcaga t
//
LOCUS       DAOPQG010000100        12280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_5975_length_12280_cov_13.681145, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000100 DAOPQG010000000
VERSION     DAOPQG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5975_length_12280_cov_13.681145"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1259
                     /locus_tag="PKI07_10815"
     CDS             186..1259
                     /locus_tag="PKI07_10815"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51581.1"
                     /translation="MTQVATGPDPCPIKTSRLRRAAAKVLLLTVTLAVGLSLMELVVR
                     WRFPYFCPAAQVPFRLVTNGLAVGPAGQTVRMATPKGDYNSVLRFNADGFRDEKRVTE
                     ATEGDWFALGDSYTMGWGVEENERFSNQLEQEFKAHELPTRVFNIAIPDNFIGYGRLL
                     RYAEGCGARVRRMVVGVCMENDLRDYRDGLGAWDLMGQPAATSKEGLRRWIKEHSALY
                     IAASFELQRFGPTRRLLHKLGIARSIDELTGHNVWNETVLQASRDELIKLGTGREMLV
                     LLIPSRNLWHGAHIATEQRVHETFARMLREAGLSVLDVRPLMEAAGDPLSCYFKTDPH
                     WNARGHALAGRALFKAIQEGSFP"
     gene            1304..3244
                     /locus_tag="PKI07_10820"
     CDS             1304..3244
                     /locus_tag="PKI07_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51582.1"
                     /translation="MSFLPLIRIWIWVSALATLAGWSLSAVGQLNRGGYLVFGGLAAA
                     AFLLGRRGSGEKRPGTGFKWRKVKARFRRWLPACFLGLTLLIFLGGALYPPSNHTAMT
                     YRTPRVLHWLMEGHWHWIDTPNYRLNNRACGLEWLTAPLLLFTKSDRGLFLVNFIPFL
                     LLPGLVFSLFKRLGVRGRVAWHWMWLLPTGYSFLVQAGGAGNDTFPAVYALAAMDFGL
                     RAWESRRASDLWLSFLSAALLTGAKASNLTLLLPCAIVVLPLLPQLKRRLGATGLVIL
                     LAGVVSFLPTALLNIRYCGDWSGLVLERAGMDMKNPLVGIWGNALLLFTNNFVPTFFP
                     LAGWWNRSALALLPRAIAGPLEANFEGGFMMLGEMPTEDWAGLGFGVSVLLAASVGAS
                     LWRRGAPAGGGSRAGPPGIPGWVRWGALLSPWVSLLAYCVKSGMVTPARLISPYYALL
                     FPVLLLGTRPLAVVRSRWWRWLEGGTLLLALLVIVVTPGRPLWPARTLLTKVLVAHPE
                     QRTAQRALAVYSVYDQRWDPLAEVRARLPQGLTVVGFMGNADDIDISLWRPFFTRRVK
                     HFLPEDTPAEIRRRGIQYAVVNGGNLAAIGLPLAAWQERTGAEVLDTITATLTVSHGP
                     QPWHIVRFPAEPGESRPEPGNE"
     gene            3292..4299
                     /locus_tag="PKI07_10825"
     CDS             3292..4299
                     /locus_tag="PKI07_10825"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOC51583.1"
                     /translation="MPEPVPTVPSDANGVLEDFRIEFMDGWRRLPNKGLFLLLLAAWL
                     ALFHFLGNATLGYIPTPSLLGWMHAAYASTPDANGTDDSHGRLIPFAVLALFWWKRKT
                     LLALPLRTWWPGLLLVVLALGLHLVGYMVQQPRISIMALFVGVYGLMGLCWGPAWLRE
                     SFFPYILFAFCIPLGWSAITITFPLRMLVIRLVEIICHNLLAIDIIVQGTSIFDPTGR
                     YQYEVAAACSGMRSLIATLAVAVIYAMVSFRSWWRRGVLVAAAVPLAVLGNVVRMLTI
                     VIAAEIGGQEWGAYIHEGGPGGIFSLLPYAAAFAGLLLLGSWLHEPSPKAPPPPATPN
                     G"
     gene            4370..5113
                     /locus_tag="PKI07_10830"
     CDS             4370..5113
                     /locus_tag="PKI07_10830"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HOC51584.1"
                     /translation="MNRRSWTLLIIVLALIGGAAALLARLQGIQKLGRPAVKTSPIPG
                     SNRLKVELPARVLDYTSEESEPDQRTLDWLPADTSFGQRLYRAPDDFMISVGVVLMGS
                     DRTSLHKTEFCIEGAGWRIDRGATEETQVHLERPYPYDLPLMKFIGTREGTFEGQPVR
                     TRGIYLVWFVADNNEYTARHWQRMWWMARDLLTHGVLQRWAAISVLAACAPGQEDATF
                     ERMKTFLQAAVPEFQLVPRRPDPAAPAAP"
     gene            5202..6599
                     /locus_tag="PKI07_10835"
     CDS             5202..6599
                     /locus_tag="PKI07_10835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOC51585.1"
                     /translation="MLRRDRQIRMQIHQLMDAILCALSFWLAYKLRANLDIIDLFGFV
                     PWAVPFGAYVGLYLVLIPAAPLILEGQGFYTRPPFCSRATTAWTLFKGCFFTCLGLVL
                     AMFLFKVPPVARVVVIWFGVIWFGLVFLKEELTRGIAANRIAQAQYRRRLLLVGESEE
                     IARIESQLQARSAAEVEIVERLDLKTTPVQHLVEMLHEHAVNGVILSARHTYFEQVEA
                     AIRACETEGVEVWLVADFFKAKISRTSFDDFYGWPVLVFRSAPESSWPRVIKQMMDTF
                     GSLVLLICLSWMFALIALLIKLTSPGPVLFRQQRSGINGRPFTIYKFRTMVTNAEQLK
                     HELDAMNEMTGPVFKVSNDPRITPIGRFLRKYSLDEFPQLFNVLCGEMSLVGPRPLPV
                     DEVKRFYDLAHRRRLSVKPGLTCLWQIRGRSNVTDFQEWVRLDLEYIDNWSLWLDFKI
                     LCGTLPAVLSGAGAK"
     gene            6631..7599
                     /locus_tag="PKI07_10840"
     CDS             6631..7599
                     /locus_tag="PKI07_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC51586.1"
                     /translation="MKQRRKTLPRSRPVSVVTGAAGFLGSHLTDRLLARGHQVIGIDN
                     FVTGSVDNIAHLGGNPRFKFIQQDVTEFLFLDTRVDYVWHFASPASPIDYLELPIQTL
                     KVGSLGTHKALGLAKNKRARFLLASTSEVYGDPLVHPQTEDYWGHVNPIGPRGCYDEA
                     KRFAEALVMAYRQEHRVETRIVRIFNTYGPRMRLNDGRVVPAFISQALKQRPLTVFGQ
                     GDQTRSFCYVSDLIEGIYRLMMSDYDLPVNIGNPTEMTMLEFAHAVIRATRSRSKITF
                     RPLPQDDPRQRRPDITRAKKILRWSPRVPLATGLVKTIDYFRLKVR"
     gene            complement(7723..8514)
                     /locus_tag="PKI07_10845"
     CDS             complement(7723..8514)
                     /locus_tag="PKI07_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51587.1"
                     /translation="MGASRNSQAVAALAFGLGLCVAAGAAGADEPPLPAGFAAAARQS
                     YAEAQAAYQRAPTEVTAAWQLARACFDQAEFATNKTERASLAEQGIAVCQRALAHATN
                     SAPLHYYLGMNLGQLARTRTLGAFKLVSQMEREFIQAGELDRQFDHAGPDRNLGLLYR
                     DAPAFGSIGNRSKAREHLARAVQLAPRYPENRLNLVESCLKWGESREAGRELAALEAI
                     WPAARTNLTGAAWAASWADWEPRRQRLKDRIKLPPEAAERPSRQR"
     gene            8568..8747
                     /locus_tag="PKI07_10850"
     CDS             8568..8747
                     /locus_tag="PKI07_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51588.1"
                     /translation="MAICVSLLAAGAGCSGINHTQSVSPATFLLPGLLRADPPPAHPD
                     LIRPTETPPLEVALF"
     gene            8758..10137
                     /locus_tag="PKI07_10855"
     CDS             8758..10137
                     /locus_tag="PKI07_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3463 domain-containing protein"
                     /protein_id="HOC51589.1"
                     /translation="MRFPLALTVKIARHILKHKLRRTPRFALVLQLEPLHTCNLSCTG
                     CGRIREYSTSLKNLMPLADCLASARECDAPMVSVCGGEPLLYPQIEELVAGLRAQRRI
                     VYICTNGLLMRKKLKDYLAATYSPRQEPVLAQLLAGQLIADSDAAFIRQGNRNGRPVI
                     APSSWLYWNVHVDGLEYTHDLIVEREGVFKECVAAIRMAKLLGFQVATNTTVYRETDV
                     GEIEQMFQFFASLGVDGHTISPGYDYDAAKQDMIRRRGRRPEDFFLTRALTIEKFARI
                     QEWSRRFNIFGTPVYQEFLAGQRALTCTAWAIPTRNIKGWKAPCYLITDAHYARYQEM
                     LDQVDWSRYGVVNGVARDPRCENCMVHCGYDPSGALGVNYQRGDTWKNIRYNFGPRPK
                     PNPAAPPVKAFNGISIGKGHRAAAGAASGPNAAGAQSDAPDIPRPGESGRSRLAPPDV
                     SPAPLRPAG"
     gene            10141..11613
                     /gene="hpnJ"
                     /locus_tag="PKI07_10860"
     CDS             10141..11613
                     /gene="hpnJ"
                     /locus_tag="PKI07_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HOC51590.1"
                     /translation="MQTLFLSPPSFDGFDGGAGSRYQARREVTSYWYPTWLAQPAALV
                     PHSKLLDCPPHGLDIKYCLETARHYDHVIIHTSTPSLKNDCKVAEAIKRNRPETKIGF
                     VGAHAAVLPAETLKASPAIDWVGRKEFDYTCLEVAEGRPLETIAGLSYRDREGRIRHN
                     PEREIITDMDALPWVADVYKRDLQIEKYYIGYLLHPYLSLYTGRGCPAQCTFCLWPQT
                     IAGHKYRVRSPQNVAAEMAYLKRLFPQVREFFFDDDTFTANLPRAREIAQRLRPLGLA
                     WSCNSRANLDADTVRFFKDCGLRLFLVGYESGNAQILKNIKKGVTLEEMRRFTRACHD
                     AGVVIHGTFVLGLPIETPQTIEESIRFAQELDMFSMQVSLAAPYPGTELYEMARQNGW
                     FVKRDKTDLVEPDGFQQSALEYPGLSKDAIFEAVDRFYRAYYLRPKPILRIIKTMLED
                     KEVFVRRCREGFEFFKSLRQRKADLAAARSGASTPAPAGV"
     gene            11783..12022
                     /locus_tag="PKI07_10865"
     CDS             11783..12022
                     /locus_tag="PKI07_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51591.1"
                     /translation="MLYRGSGRILLVMLADEKTSPEQIEIFRAMTGEQRLRLAEQLYW
                     SARKLKLAGLRAQHPDWTEEQLNAAVRQVFRHART"
BASE COUNT         2030 a         3941 c         4005 g         2304 t
ORIGIN      
        1 aaaagacgga tattggaggg agttttgctg ctggcgtggg gacgccgggc cgagggtttg
       61 ccgggcagcg ccttggcatt ttggccgcag gccggcatgg gcggatgtac cagttcgctt
      121 ttcttgaggc gttttggggt tcaggattga tgtccggcgg agatttggct aggttggcag
      181 gacttatgac gcaggttgcg acggggccag atccctgccc gatcaagact tcccggctgc
      241 gccgtgcggc ggcgaaggtt cttttgctga ccgtgacgct tgccgtgggg ctgtcgctga
      301 tggagttggt ggtgcggtgg cggtttccct atttctgccc ggcggcgcag gtgccttttc
      361 ggctggtgac taacggcctg gcggtcggcc cggcgggcca aacggtgcgg atggcaacgc
      421 cgaaggggga ctacaattcc gtgctccggt tcaatgcgga tggctttcgc gacgaaaagc
      481 gggtgacgga ggccacagag ggggattggt ttgcgttggg ggactcctac accatgggct
      541 ggggggtcga ggaaaacgag cggttttcga accagttgga acaggagttc aaggcgcatg
      601 agctgccgac gcgggtgttt aacatagcca tccccgacaa cttcatcggc tatgggcggc
      661 tgctgcgcta tgccgagggc tgcggggccc gggttcggag gatggtggtg ggggtctgca
      721 tggagaacga tttgcgtgat taccgcgacg ggttgggggc gtgggacttg atgggccagc
      781 cggcggcgac gagcaaggag ggtttgcgaa ggtggatcaa ggagcatagc gcgctgtata
      841 tcgcagcttc gtttgaattg cagcggtttg ggcccacccg ccggttgctg cacaagctgg
      901 gcatcgcgcg cagcattgac gaactgacgg ggcacaatgt ctggaatgaa acggtgctcc
      961 aggccagccg ggatgagctg atcaagctgg gaacggggcg cgagatgctg gtgctcctca
     1021 tcccctcgcg caatctctgg cacggcgcgc atattgccac cgagcagcgt gtccacgaga
     1081 cattcgcgcg gatgctgcgc gaggccggcc ttagcgtgct ggatgtccgg cccctgatgg
     1141 aggccgccgg cgacccgcta agctgctact tcaagacgga tccccattgg aacgcgcggg
     1201 gccacgcgct ggccgggcgc gcgttgttta aggccattca ggaggggtcg tttccatgag
     1261 cggccccggg cgggcgcaga gggtggcggg catgttctag ccgatgagtt ttctgccgct
     1321 catcaggatc tggatttggg tttccgcgct ggccaccctt gcggggtggt ctctgtcggc
     1381 agtggggcag ttgaaccggg gaggctacct ggtctttggc ggcctggcgg cggcggcgtt
     1441 tctgctgggt cgccggggct ccggcgagaa gcggccggga acgggcttta agtggcgcaa
     1501 ggtgaaggcg cgcttccggc ggtggctgcc ggcctgtttc ctggggctga ccctgctgat
     1561 ctttctcggc ggagcgctat atccgccgag caatcatacg gccatgacgt atcggacgcc
     1621 gcgcgttttg cactggctga tggaagggca ctggcattgg attgacaccc ccaactaccg
     1681 gctcaacaac cgggcgtgcg gcctggaatg gctgaccgcg ccgctgctgc tgttcacgaa
     1741 gtccgatcgc ggcctgttcc tggttaactt catcccgttt ctcctgctgc ccgggctggt
     1801 gttcagcctg tttaagcggc ttggcgtgcg gggccgggtg gcctggcact ggatgtggct
     1861 gctgccgacg ggatacagct ttctggtgca ggccgggggc gccgggaacg acaccttccc
     1921 ggcggtctat gcgctggccg cgatggactt tggcctgcgg gcctgggaat cgcggcgggc
     1981 gtcggacctg tggctttcgt tcctgtcggc ggccttgctg accggggcca aggcgagcaa
     2041 cctgacgctg ctgctgccct gcgcgattgt ggttctgccg ctgctgccgc aattgaagcg
     2101 gcggctgggg gccaccgggc tggtgatcct gctggcgggg gtggtctctt tcctgcccac
     2161 ggcgctgttg aatatccgct attgcgggga ttggtcgggc ctggtgctcg aacgggcggg
     2221 catggatatg aagaacccgc tggtggggat ttggggcaac gcgctgctgt tgttcacgaa
     2281 caactttgtg cccacctttt tccccctggc ggggtggtgg aaccgctcgg cgctggccct
     2341 gctgccgcgc gcgatcgccg ggcccctgga ggcgaatttc gagggcggct tcatgatgct
     2401 gggcgaaatg ccgaccgaag actgggccgg cctgggattc ggggtgagtg tgttgctggc
     2461 ggccagcgtg ggggcgtcgc tgtggcggcg cggggccccg gccgggggcg ggagccgggc
     2521 gggccctccg gggattccgg gctgggtgcg ttggggcgcg ctgttgtcgc cgtgggtatc
     2581 gctgctggct tattgtgtga agtccggcat ggtgacgccg gcgcggctga tttcgcccta
     2641 ctatgcgttg cttttcccgg tattgctgct ggggacgagg ccgctcgccg tggtccgatc
     2701 ccggtggtgg cgctggctgg aaggcggcac cctgctgctc gcgctgctgg tgatcgtcgt
     2761 gacgccgggc cggcctttgt ggcccgcccg gactcttttg acgaaggtgc tggtggcgca
     2821 tcccgagcaa cgaaccgcgc agcgggccct ggcggtttac agtgtttatg accagcgctg
     2881 ggatccgctg gcggaggtgc gggcgcggct gccgcaaggg ttgacggtgg tggggttcat
     2941 ggggaatgcc gatgacatag atatttccct gtggcggccc tttttcaccc ggcgggtgaa
     3001 gcactttttg cccgaagata cgccggcgga aatccggcgg cgcggcattc aatatgccgt
     3061 ggtgaatggc ggcaaccttg cggcaatcgg cctgcccctg gccgcctggc aagagcggac
     3121 gggggcggag gtgctggaca ccataactgc caccctgacg gtgagccacg ggcctcaacc
     3181 gtggcatatc gtgcgctttc cggcggaacc gggggaaagc cgccccgagc cgggaaacga
     3241 ataagattga gcattccggc gccgcggtgt atccaataga ttcaattaat tatgcccgaa
     3301 ccagtgccaa ccgtgccatc tgacgccaac ggcgttctcg aggacttccg gatagagttc
     3361 atggacggct ggcggcgtct gccgaataaa gggcttttcc tgctgctttt ggcggcctgg
     3421 ctggcgctgt tccatttcct gggcaatgcc accctgggtt acatcccgac gccgtcgctg
     3481 ctgggctgga tgcacgcggc ctacgcctcg accccggatg ccaacggcac tgacgacagc
     3541 cacggccggc tcattccgtt cgcggtgctg gcgcttttct ggtggaaacg gaagacgctg
     3601 ctggcgctgc cgctgcggac ctggtggccg gggctgctgc ttgtggtcct ggcgctcggc
     3661 ctgcacctgg tgggctacat ggtgcagcag ccgcgcatct ccattatggc gctgtttgtc
     3721 ggagtctatg ggctgatggg cttatgctgg gggcccgcct ggctgcgcga aagtttcttc
     3781 ccctacatcc tgttcgcctt ctgcatccct ttgggctggt ccgcgataac cattaccttc
     3841 ccgctgcgca tgctggtgat ccggttggtg gaaatcatct gccataacct gctggcgatt
     3901 gatattatcg tgcaggggac atccattttc gatcccaccg ggcggtacca atacgaggtg
     3961 gcggccgcct gcagcgggat gcgcagtctc atcgccacgc tggcggtggc ggtgatctac
     4021 gcgatggttt ccttccggag ctggtggcgg cgcggggtgc tggtggcggc tgcggtgccc
     4081 ctggcggtgt tgggcaacgt ggtgcggatg ctgacgatcg ttatcgccgc cgagatcggc
     4141 gggcaggaat ggggcgctta catccatgaa ggcggccccg gggggatttt cagcctgctg
     4201 ccgtatgcgg ccgcgtttgc gggcctgctg ctgctgggaa gctggctgca tgagccctcg
     4261 cccaaggcgc cgccgccgcc cgccaccccg aacggctgag ccggcttccc cccgccccga
     4321 atgcccaaat gaaacgccgg ctgctgatgg ttttaaacgc gcatacgaca tgaaccgacg
     4381 atcgtggacc ctcctgataa tcgtgctcgc cttgatcggc ggcgccgccg cgctgctggc
     4441 gagactgcag ggcatccaga aactgggccg gcccgccgtg aagaccagcc ccatccccgg
     4501 cagcaaccgg ctcaaagtgg agctgccggc gcgcgtgctg gattatacct cggaggaatc
     4561 cgagccggac cagaggacgc tcgactggct gccggcggac accagttttg gccaaaggct
     4621 gtaccgggct ccggatgact ttatgatctc cgtcggcgtg gtgctgatgg gcagcgatcg
     4681 caccagcctg cataagacgg aattctgcat cgaaggcgcg ggctggcgca ttgatcgcgg
     4741 cgccacggag gagacccagg tccacctgga gcggccttac ccttacgatc tgccgctgat
     4801 gaagttcatc ggcacccggg agggaacgtt cgaaggccag ccggtgcgca cccggggcat
     4861 ttacctggtt tggttcgtgg cggataacaa cgaatacacg gcgcggcatt ggcagcggat
     4921 gtggtggatg gcccgggacc ttctgaccca cggcgtcctg cagcgctggg cggccatcag
     4981 cgtcctggcc gcgtgcgcgc cgggacagga agacgccacc tttgagcgca tgaagacctt
     5041 cctgcaggcc gcggtgccgg aattccaatt ggtgccgcgc cggccggacc cggcggcgcc
     5101 cgccgcgcca tgaaccccgg cgcgcccagg gtcgaacgcg gcaaaaatat gctcgacgaa
     5161 ccccgccgct ccgggctgaa atcaggttcg ggctaacaga tatgttgcgt cgcgaccgcc
     5221 agattcggat gcaaatccac caactgatgg atgccattct ttgcgccctc agtttttggc
     5281 tggcctacaa gctgcgcgcc aacctcgaca tcattgatct gttcgggttc gtgccctggg
     5341 cggtgccgtt tggggcttac gtcgggttgt acctggtcct cattcccgcc gcgccgctga
     5401 tcctggaggg ccagggattc tacacgcgcc cgcccttctg ttcgcgggcc accacggcct
     5461 ggacgctctt caaaggctgc ttctttacgt gcctgggcct ggtgctggcg atgttcctgt
     5521 tcaaggtgcc cccggtggcc cgcgtggtgg tgatctggtt cggcgtcatc tggtttgggc
     5581 tggtgtttct caaagaggaa ttgacgcgcg ggatcgccgc caaccgaatc gcgcaggcgc
     5641 aataccggcg gcggctgctg ctcgtgggcg agtccgagga aatcgcgcgg atcgaatccc
     5701 agttgcaggc gcggtccgct gcggaggtgg aaatcgtgga acggctggac ttgaagacga
     5761 ccccggtgca gcacctggtg gaaatgctgc acgagcacgc cgtgaatggg gttatcctca
     5821 gcgcccggca tacctacttt gagcaggtgg aagccgccat ccgcgcctgc gaaaccgagg
     5881 gcgtcgaggt ctggctggtg gcggatttct tcaaggccaa aatctcccgg accagcttcg
     5941 acgatttcta cggctggccg gtgctggttt tccgctccgc gcccgagtct tcgtggccgc
     6001 gggtgattaa gcagatgatg gacaccttcg gctcgctggt gctgctgatc tgcctgtcgt
     6061 ggatgttcgc gctgattgcg ctgctcatca agctgacctc gccggggccg gtgctgtttc
     6121 ggcagcagcg ctccggaatc aacggccggc ctttcaccat ctacaagttt cgcaccatgg
     6181 tgaccaatgc cgagcagctc aaacacgagc ttgatgccat gaatgaaatg accggcccgg
     6241 tcttcaaagt gagcaacgac ccgcgcatca cgcccatcgg caggttcctc cgcaagtaca
     6301 gcctcgatga gttcccgcag ctctttaacg tgctgtgcgg cgagatgagc ctcgtggggc
     6361 cgcgcccgct gccggtggac gaagtcaagc ggttctacga cctggcgcac cggcgccgcc
     6421 tgagcgtcaa gcccgggctg acctgcctgt ggcaaatccg cgggcgcagc aacgtcaccg
     6481 acttccagga atgggtgcgg ctggacctgg agtacattga taactggtcg ctttggctcg
     6541 acttcaagat cctctgcggc accctgccgg ccgtgctgtc cggcgcgggc gccaagtaag
     6601 ccgcccggaa accgccgcgc ttaaattatt atgaaacaac gccgcaaaac gctccctcgc
     6661 tcccggccgg tctcggtcgt caccggcgcc gccggcttcc ttgggtcgca cctcacagac
     6721 cggctgttgg cgcgcggaca ccaggtcatc gggattgaca attttgtcac cggctcggtg
     6781 gataacatcg cgcacctggg aggaaatccc cgcttcaagt tcatccagca ggacgtgacc
     6841 gagtttcttt tcctggatac gcgggtggat tatgtgtggc actttgcgtc gccggccagt
     6901 cccattgact acctggagct gcccatccag accttgaaag tcggctcgct gggcacccac
     6961 aaggccctgg ggctggccaa gaacaagcgg gcgcgctttc tcctggcttc gacctcggaa
     7021 gtgtacggcg atcccctggt ccacccgcag accgaagact actggggcca cgtaaacccg
     7081 attggcccgc gcggctgcta cgacgaagcc aagcgcttcg ccgaggcgct cgtcatggct
     7141 tatcgccagg agcaccgggt cgaaacccgc atcgtgcgca tcttcaacac ctacggcccc
     7201 cgaatgcgac tgaacgacgg ccgggtcgtg ccggcgttca tcagccaggc gctcaagcag
     7261 cggccgctca ccgtgtttgg ccagggcgac cagacccgca gcttctgcta cgtctcggac
     7321 ctgatcgaag gcatctatcg gctgatgatg agcgactatg atctaccggt gaacatcggc
     7381 aaccccacgg aaatgaccat gctggagttc gcccacgccg tcatccgcgc cacccgctcc
     7441 cgcagcaaaa tcacgttccg cccgctgccg caggacgatc cccgccagcg ccggccggac
     7501 attacccgcg ccaaaaaaat cctccgctgg tcgccccgtg tgccgctggc gacgggcctg
     7561 gtcaagacca ttgattattt tcggctgaaa gtgcggtagg ccgggcggag aaggcgctag
     7621 cgctcccggc ggacgacgat ggccccgtcc ccgcacgcgc ccctggccac gcccggggcc
     7681 gcctcgaaaa tccgcgccgc cggagccaca agattaaccg gctcaacgct ggcgggaggg
     7741 tcgctcggcc gcctcgggag gcagtttgat tctatccttg agcctttgcc ggcgcggttc
     7801 ccaatccgcc caactcgccg cccatgcggc gcccgtcagg ttggtgcgcg cggcgggcca
     7861 gatggcttcg agcgcggcca actcgcgccc ggcctcgcgg gattcccccc atttcaggca
     7921 ggactcaaca aggttcaacc ggttctcggg ataccgcggg gccaactgca ccgcgcgcgc
     7981 caggtgttcg cgcgccttgc tgcggttccc gatgctgcca aaggcggggg catcgcggta
     8041 gagcagcccg agattgcggt cggggccggc gtggtcaaac tgccgatcca actcgccggc
     8101 ttggataaat tcgcgctcca tctggctgac cagcttgaac gcgcccaacg tccgggtgcg
     8161 ggccagttgc ccgagattca tcccgaggta gtaatggagc ggagccgaat tggttgcgtg
     8221 cgccagcgcg cgctggcaaa ccgcgatccc ctgctccgcc agggaggcgc gctcggtctt
     8281 gttggtggca aactccgcct ggtcaaagca cgcccgggcc agttgccagg ccgccgtcac
     8341 ctccgtcggc gcgcgctggt aagccgcctg cgcctccgcg tagctctgcc gcgcggcggc
     8401 ggcgaacccg gccggcaaag gcggctcgtc cgcgccggcg gcgcccgccg ccacgcacag
     8461 gcccaggcca aaagccagcg cggccaccgc ctgcgaattg cggcttgcac ccatcctgac
     8521 ttgagctatg ctaatcagag ttgaaattag actggaaaat tgcacggttg gcaatatgcg
     8581 tgagcctcct ggcggccggt gccggctgca gcggcatcaa ccacacgcaa agcgtctcgc
     8641 ccgccacttt cctcctgccg ggactgttgc gagccgaccc gccgccggcc cacccggacc
     8701 tgatccggcc cacggaaacg ccgcccctcg aagtcgccct gttctgagtc cggtgccatg
     8761 cgatttccgc tcgcgttgac ggtcaaaatc gcccgtcata tccttaaaca taaattacgg
     8821 cgcaccccgc ggtttgcgct cgtgctgcag cttgaaccgc tgcatacctg caacctctcc
     8881 tgcaccggtt gcggccgcat ccgcgagtat tccacgagcc tgaagaatct catgccgctg
     8941 gcggactgcc tggcgtcggc gcgggaatgc gacgcgccaa tggtctcggt ctgcggcggg
     9001 gaaccgctgc tctatcccca gatcgaggaa ctggtcgcgg gcctgcgggc gcaacgacgc
     9061 attgtctata tttgcaccaa tggcttgctc atgcgcaaga aactcaagga ttacctggcg
     9121 gcaacctatt cgccgcggca ggagccggtt ctggcgcagt tgctggccgg gcagctcatc
     9181 gcggattcgg acgccgcttt catccgccag ggcaaccgca atggacggcc tgtcatcgcg
     9241 ccctcctcgt ggctttattg gaatgttcac gtggacggcc tggaatacac ccacgacttg
     9301 atcgtcgagc gggaaggcgt gtttaaggag tgcgtcgcgg ccatccgcat ggcgaagctc
     9361 ctcgggttcc aggtggcaac caacaccacg gtgtaccgcg agaccgatgt cggggaaatc
     9421 gagcagatgt tccagttctt tgcctcgctc ggcgtggatg gccacactat ttcccccggc
     9481 tacgactacg acgccgccaa gcaggacatg attcggcgcc gggggcggcg gccggaggat
     9541 ttcttcctca cgcgcgccct gaccatcgag aagtttgccc ggatccagga gtggagccgg
     9601 cggttcaaca tctttggcac gccggtttac caggaattcc tggccggaca gcgcgcgctg
     9661 acttgcaccg cctgggccat tcccactcgc aacatcaaag gctggaaagc tccctgttat
     9721 cttatcaccg acgcccatta cgcccgctac caggaaatgc tggaccaggt ggactggtcc
     9781 cggtatggcg tggtgaatgg cgtggcgcgc gacccgcgtt gcgagaactg catggtgcac
     9841 tgcggctacg accccagcgg cgccctcggc gtcaactatc agcgcggcga tacctggaag
     9901 aatatccgct acaatttcgg cccccggccc aaacccaacc ctgccgcccc cccggtgaag
     9961 gcgttcaacg ggatttccat cggcaaaggc catcgcgccg cagccggagc cgcctccggc
    10021 cccaatgccg cgggcgccca atccgacgcc ccggacatac cccgcccagg agaaagcggc
    10081 aggagccgcc tcgcgccacc ggacgtttcc ccggcgcccc tccgcccggc aggctagacc
    10141 atgcaaaccc tgtttctttc ccctcccagc ttcgatggtt ttgacggcgg cgccggctcg
    10201 cgctaccagg cgcgccgcga agtgaccagc tactggtatc cgacctggct cgcccaaccg
    10261 gcggcgctgg tgccccactc aaagctgctg gactgccccc cgcacggcct ggacatcaaa
    10321 tactgcctgg aaacggcccg ccactacgac cacgtcatca ttcatacttc aaccccttcg
    10381 ttgaaaaacg attgcaaggt tgccgaagcc atcaaacgga accggccgga aaccaaaatc
    10441 ggcttcgtcg gcgcgcacgc ggcggtcctg ccggctgaaa ccctcaaggc gtccccggcc
    10501 attgactggg ttgggcggaa ggaattcgat tacacctgtt tggaagtcgc cgaggggcgc
    10561 ccgctggaga ccattgccgg cttgagctac cgcgaccgcg aggggcggat ccggcataat
    10621 cccgaacgcg aaatcatcac ggacatggac gccctgccgt gggtggcgga tgtctataaa
    10681 cgcgacctgc agatcgaaaa atactacatc ggctacctcc tccatcccta cctcagcctc
    10741 tacaccgggc gcggctgccc cgcccaatgc accttctgcc tctggccgca aaccatcgcc
    10801 ggacataaat accgcgtgcg ctccccgcag aatgtcgccg ccgaaatggc ttacctgaaa
    10861 cgcctcttcc cgcaggtccg cgagttcttc ttcgacgacg acaccttcac cgccaacctg
    10921 ccgcgcgccc gcgagatcgc ccaacggctt cgcccgctcg gcctggcctg gagctgcaac
    10981 agccgcgcca acctcgacgc cgacaccgtg cgctttttca aggattgcgg cctgcgcctg
    11041 tttctggtcg gctacgaaag cggcaacgcg cagattctca aaaacatcaa gaagggggtc
    11101 accctggagg aaatgcgccg cttcacccgg gcctgccatg acgccggcgt cgtcatccac
    11161 ggcaccttcg tcctgggcct gcccatcgaa acgccgcaaa ccatcgaaga gagcatccgc
    11221 ttcgcgcagg aactggacat gttcagcatg caagtcagcc tcgccgcccc gtaccccggc
    11281 actgaactgt atgaaatggc ccgccaaaac ggctggttcg tcaaacggga caagaccgac
    11341 ctggtggagc ccgacggctt ccagcaaagc gccctcgaat acccgggcct gagcaaggac
    11401 gccatcttcg aggccgtgga ccgcttctac cgcgcctatt acctgcgccc caagcccatc
    11461 ctgcgcatca tcaagaccat gctcgaagac aaggaagtct tcgtccgccg ctgccgcgag
    11521 ggcttcgagt tcttcaaaag cctccgccag cgcaaggcgg atttggccgc cgccagaagt
    11581 ggcgcctcaa cacccgcgcc ggcgggagtg tgacgctaca gcgccgtcgc gcgcgaccag
    11641 gtcatttcct ccggcccgag ccattgggca tagcagtttc atagcatgcc cgggctcgct
    11701 ggccctcatg gtcttccgtc cacgggaaag tgccccagtc cctattcccg ccgcaccgcg
    11761 tagcggcatc tgcccgtcgg gcttgcttta tcgcggctcc gggcgcatcc tcctggtgat
    11821 gctggccgac gagaaaacca gtcccgagca gattgaaatc ttccgcgcca tgaccggcga
    11881 gcagcggctg cggttggccg agcagctata ctggtcggca cgcaagctca agctggccgg
    11941 cctgcgcgcg cagcatcccg actggacaga ggagcaactc aacgccgcgg tgcggcaggt
    12001 tttccggcat gcccgaacct gattcagcgg ctggtcgagc tcttccccgc ggctgatttc
    12061 tacctgtcgc cggtggaagt catcgccgcg gaggcccgcg ggaacaacga ggccgcttca
    12121 acatcatcca cctcggaacc ggcttcaaag ccggctgcta cacgaccggg cgggatgagc
    12181 tgcacgcctg ggcgtttcga ctgaagcgtt ccgtccagtc gagggggaaa cggtgaggct
    12241 cgccccgccg gagtacgtca tccccgcaag ctgggtatta
//
LOCUS       DAOPQG010000101        12242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6001_length_12242_cov_14.298679, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000101 DAOPQG010000000
VERSION     DAOPQG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12242
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6001_length_12242_cov_14.298679"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PKI07_10870"
     CDS             <1..1292
                     /locus_tag="PKI07_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51592.1"
                     /translation="AAAINGGLLLPLHLGWWGFQSFNPPQVEPAYPDVIETLGARLIG
                     WDAGISLTAGIDREALRTTPLFRRAVDILRTSEALRHARAFDPVARAALREPGSRFLL
                     VTNAAGQPRFRRAVLQEHTLSSAEPWTLAWPVTNAFQPQPIRFRLEALMSAAQPDDTN
                     ALLLVDLARTNGGYWKATSADGVACRWAPGDGQREAGGWLAATNAGKVACNAAWTRLE
                     HRFDPLLNLKEQPALSFEVDGDGSGAVVAIRLESPRAIAYGAVADRYLDVNFTGPRTF
                     TLVETESTRWSDYVWNDGKHPYNVYRETIDFGAIESVSLWLQNLPPGRETRCRLGPVR
                     AVPLRAGRLRNPRLTVGGQTVEFPIELASGCWLEGTGPDDCAAYGARGEALGKVTPRG
                     DWPRLPAGVVAMQFACESAASPAPRARLTVFCQGADL"
     gene            complement(1514..4033)
                     /locus_tag="PKI07_10875"
     CDS             complement(1514..4033)
                     /locus_tag="PKI07_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51593.1"
                     /translation="MMASPAGKVPLRFPVWLLAAALALATLALYWPATGHDFISFDDT
                     RYVTENPQVRSGLTWEGVKWAFRHPLLCNWHPVTVLSHMLDGQVYGLAPWGHHLTSVL
                     LHALNALLVFVGLRQMTGARGRSLLVAALFAFHPLRVESVAWVAERKDVLSGSFGLLT
                     LICYARYAADSRLSALHGASARTRHSSARFYFLSLGCFALGLMSKATLVTWPFVLLLL
                     DYWPLMRMASCARQEAERGAAVPTSGRVVPWGRLIGEKIPFFALAAVMSVVTYAVQAG
                     AMPGAGKFPLSARVANALVSYSRYLGKQFWPTDLALFYPYPGHWPLAAVLGSGGLMLG
                     VSGLAFMWRRRAPFLLVGWLWFVGTLVPMIGLVQVGGQAMADRYTYLPTLGLLVLVVW
                     GAHALFRRWRGAGVAWAMAALAFCLVLTRQQLGHWQESETVFRHAAAVTRDNYVAYKA
                     LGDALAGKRKMDEAIRQYEQALRLRPDDSGLHNNLGLALLQQGQTNEAIRQYQHALRL
                     QPDNAHAHNNLGIALASLGQTNEAQQQFETAIRLQEDFAEARRNLGRILADRGQTDEA
                     IRQFEAAIRQRPDDAETHVNLGLALGRKGRTDDAIRHYQQALRLNPGSATAHYNLGNV
                     FYERNRVDEAFLHYQEAVRRQPDDAQARCNLGIVLARKGRVDEAIQQCREALRRQPDL
                     FQAHLGLSLILLLTGQLQEAAFHLEEVLRICPREQLEAPDSPGRETIRDALNDLAWRL
                     ATHPAATERDGARAVRFAEQACALAHHQNPALANTLAAAYAGAGRFKEAIEAARQAQA
                     QALAAGLPEVAEKSRQLLELFQSNQPCREPPPPDARPGSAR"
     gene            4262..4792
                     /locus_tag="PKI07_10880"
     CDS             4262..4792
                     /locus_tag="PKI07_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOC51594.1"
                     /translation="MNDNLDERLRFIFGRRSIRAYSSAPVSEATVQQLLAAAMAAPSA
                     AGKDPWRFVVVRNRGTLAQIAGALPNGRMLAEAALGIVVAGELPAAHDQQVGYLLQDC
                     SAAIENLLLGAHLLGLGACWLGVYPREQRVDALRQILALPPTVIPVSCISLGHPAETK
                     PPRTRFTPDYVHFEKW"
     gene            complement(4829..5263)
                     /locus_tag="PKI07_10885"
     CDS             complement(4829..5263)
                     /locus_tag="PKI07_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOC51595.1"
                     /translation="MSIIEALLAEHVVFLRVFDQIERALPSLVTLAEVRTLAGMVEAL
                     LEEHARTETELAYLALDHVLEQSGELQSLHQEHQEIDDRLRRVHTAKTCAEARRLLKA
                     ALTTSREHFRGEEKHVFPLLAKCLRAETLLELGQNWRQRQAA"
     gene            5379..6338
                     /locus_tag="PKI07_10890"
     CDS             5379..6338
                     /locus_tag="PKI07_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOC51596.1"
                     /translation="MHIPDGFIDGKTAAATALLSAAGVGLALRQVRRRLPPRRVPLLG
                     LAAAFLFAAQMVNFPVAAGVSGHLIGGVLVAALLGPSAAVVVLTTVLIVQCFLFADGG
                     VLALGANVFNMALVGAVGGCAIYHGVRRGWPGRRGQVAAVAFAGWCSTVLAATCCAGQ
                     LAWSGTVAWSAAFPALTGTHMIIGVGEGLISALVLLAVLRARPDLASEPGDAASPGRG
                     GELVRYGLLAALGLALFVAPFACPWPDGLESVAARLGFEHRAAPPAIAAPAADYQAPG
                     VSWAVGATALAGAAGTVIVFGLAGLLAWSLVPRPEEKARAGKP"
     gene            6335..6949
                     /locus_tag="PKI07_10895"
     CDS             6335..6949
                     /locus_tag="PKI07_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HOC51597.1"
                     /translation="MIDHWIGHYHRGPDTVVSRLPASLKLGVALAIIAGTVLAPPRAA
                     GWYLAVAAALAGAVILSRMPMRFLLKRLAWLSPLVLGVAVANVFQPGGRGNWPTVAVK
                     SMLCLATIITVSNTTPFSHILRVLRAVRVPALLITTLALMHRYLFVLAEEAERMRRAR
                     TSRLFTRPRRARWRLLSTVVGQLFVRASERAERIYGAMCARGWK"
     gene            6946..7695
                     /locus_tag="PKI07_10900"
     CDS             6946..7695
                     /locus_tag="PKI07_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC51598.1"
                     /translation="MSAAIEVNALRYRYHDGTEALRGVSFTVAPGECVGLLGPNGSGK
                     STLLLHLNGLLPERLEPRGAVRILGQPITPDNLETVRRQVGLLFQDPDDQLFCPSVAE
                     DVAFGPQQLGLGEAEVKARVERALAQTGLAGFGHRATHHLSRGEKRRACLAGVLACEP
                     GLLVLDEPTSDLDPRGRREFKALLQSIAATKLIATHDLELVVELCSRAVVLDQGVVVA
                     NGPVPELLNDEELMLAHGLERPHILRHKHPH"
     gene            complement(7922..10213)
                     /gene="feoB"
                     /locus_tag="PKI07_10905"
     CDS             complement(7922..10213)
                     /gene="feoB"
                     /locus_tag="PKI07_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOC51599.1"
                     /translation="MRKPGGAASRTPEERAGSASPTPPPPGPRYVVLTGNPNSGKTTL
                     FNALTGLRAKVGNYAGVTVERKEGRLLGVPPGANVRVLDLPGSYSLSPHSLDEQISRD
                     VLLNRLPELPAPTVIVLVVDASNLQRNLYYATQVIELGYPTLVALNMVDVAEKIGHRI
                     QPDKLAAELGVPVCPLVANTGQGMPELRRAIQAALDRPAPAGTRRFPELPPVLQAELD
                     ALAALLAQTFQERRAQAAAEALLILSNEKALASSLEHYPAPVLDAVSAARQRLETAGV
                     DWRTIGIENRYQAVAAIEQAATTELVPPGETFSDRLDHVLTHKLWGTVIFLALMTLMF
                     QGIFTFARIPMDALQTGVDWFGRTVGAALPPGDLNNLLVDGVIAGVGAVVVFMPQILL
                     LFLFIGFLEDTGYMARAAFLMDRFMSKVGLHGKSFIPMLSSFACAIPGIMATRTIESP
                     KDRLVTILVAPLMSCSARLPVYTLLIAACVPNLTVFGFLKLPGLTMLSCYLLGIGVAL
                     FMAWLFKKTLLRGDTPLLIMELPPYKRPLLRVVIRHMWDRSQIFLRRAGTVILGINIL
                     LWFLATYPRNPAANASLEARRAQLEAQAARAQTAGQTAALRAELAALDSQAAGENLRQ
                     SFAGRLGRLIEPVIAPLGFDWKIGIGIITSFAAREVFVSTMSTVYNVGRADPTHSSMN
                     SLAQTLQMQKKPDGSPVYTPLTGITLIVFYVFALQCVSTVAIVQRETNTWKWPLFQWL
                     YMGVLAWGFAYLTQLIGQWLGWT"
     gene            complement(10206..10448)
                     /locus_tag="PKI07_10910"
     CDS             complement(10206..10448)
                     /locus_tag="PKI07_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOC51600.1"
                     /translation="MPPTPLPLTSLPLGGRAVVVEINLPPESRPRLMEMGLLVGTPVE
                     LVRFAPLGDPVDIKVRGYHLTLRKHEAEQILVQPNA"
     gene            10745..11536
                     /locus_tag="PKI07_10915"
     CDS             10745..11536
                     /locus_tag="PKI07_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC51601.1"
                     /translation="MYRDQKVVVVMPAYNAAKTLRQTYAEVREQGVVDQIILVDDNSR
                     DETVAVARQLEGVLVHVHEVNKGYGGNQKTCYRLALAAAADIVIMIHPDYQYTPRLIP
                     AMVSIIGNGLHPCVLGSRILGGYALRGGMPIWKYVANRGLTLVENILLGAKLSEYHTG
                     YRAFSRAILEKLDLQGNSDDFVFDNQMLAQILWHGFTIAEVSCPTKYFPEASSINLRR
                     SIKYGCGCLATGLQYRLSKMGVLRSRLFPRMAGKAQGRSAKAPPG"
     gene            complement(11565..11828)
                     /gene="rpsT"
                     /locus_tag="PKI07_10920"
     CDS             complement(11565..11828)
                     /gene="rpsT"
                     /locus_tag="PKI07_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOC51602.1"
                     /translation="MPNTKSAERRMRSSARRNLRNRSAKSRLHTLEGAYLKQLSSGKK
                     DEAARELRTLSSALDKAAKTGVLPRATVNRKKSRLALRLAKAK"
BASE COUNT         2043 a         3824 c         4281 g         2094 t
ORIGIN      
        1 acgcggcggc catcaacggc gggctgctgc tgccgctgca cctgggctgg tggggtttcc
       61 agtccttcaa cccgccgcaa gtcgagccgg cctacccgga cgtgattgaa accctcggcg
      121 cccggctcat cgggtgggat gccggcattt cgctgaccgc cggcatagac cgtgaggctc
      181 tccgcaccac gccgctcttc cgccgggcgg tggacattct gcggaccagc gaggcgcttc
      241 gccacgcccg ggcgttcgat ccagtcgccc gcgccgccct gcgcgagccc ggcagccgtt
      301 ttctgctggt cacgaacgcc gcgggacagc ctcgcttccg ccgcgccgtg ctccaggagc
      361 acacgctttc ctccgccgag ccctggaccc tggcctggcc ggtcaccaat gcctttcagc
      421 cgcagccgat tcggttccgc ctggaagccc tgatgtccgc cgcgcaaccc gatgatacca
      481 acgcccttct gctcgtggac ctggcccgaa cgaatggcgg ctactggaaa gccaccagcg
      541 ccgatggcgt ggcctgccga tgggcgcccg gggatggcca aagggaggcc ggtggctggc
      601 tggccgccac caacgccggc aaggtcgcct gcaacgccgc gtggacccgg ctcgaacatc
      661 gcttcgaccc gctgctcaac ctcaaggagc agccggcgct cagcttcgag gtggatggag
      721 acggctccgg cgccgtcgtc gcaatccgcc tcgaaagccc gcgcgccatc gcttacggcg
      781 cggttgccga ccgctacctg gatgtgaatt tcaccggccc gcgtacgttc accctcgtcg
      841 aaaccgagtc cacccgctgg agcgattacg tgtggaacga cggcaagcat ccctacaatg
      901 tttatcgcga gacgattgac tttggcgcca tcgaatccgt cagtctttgg ctgcagaatc
      961 tccctcccgg gcgggagacc cggtgccggc ttggcccggt gcgggcggtg ccgctgcgcg
     1021 cgggccgcct gcggaatccg cgattgactg tgggcggcca gacggtcgag ttccccatcg
     1081 aactggcttc cggttgctgg ctcgagggca ccggcccgga cgactgcgcc gcctacggcg
     1141 cgcgtggtga ggcgctgggc aaagtgaccc cgcgcggaga ttggccccgg ctcccggccg
     1201 gcgtcgtggc gatgcagttt gcctgtgaat ccgccgcctc gcccgcgccg cgcgcccgcc
     1261 tcaccgtgtt ctgccagggc gcggacctct gagttggccg gcaaaagtaa gggcggcccg
     1321 gcttttacac cgaaccgccc taacccaacc caactaggac atacgggcgg cttttttttc
     1381 ggttgccgcc cgaaccatga aggtgcgggt atccgcgccc gcaccggaaa gacttatggc
     1441 atggatcgcg ccggaacgca agcgtttgtt tgcaaatttt tacgcgcgcc acttctcccg
     1501 gagaaaagcc gggttagcgg gccgagccgg gtcgggcgtc cggcggaggc ggctcgcggc
     1561 agggctggtt ggattgaaaa agctccagca attgccggct cttttccgcg acctcgggta
     1621 gcccggctgc cagcgcctgg gcctgggctt gccgggccgc ttcgatcgct tccttgaacc
     1681 ggccggcccc ggcataggcg gcggccaggg tgtttgccag ggccgggttc tgatggtggg
     1741 ccagggcgca ggcctgttcg gcgaagcgaa cggcgcgcgc gccatcccgt tctgtggctg
     1801 ccgggtgggt ggccagtcgc cacgcgaggt cgttcaaggc gtcccgaatc gtctcgcggc
     1861 ccgggctgtc cggcgcttcg agttgctcgc gagggcaaat gcggaggact tcttcgaggt
     1921 ggaacgcggc ttcctggagt tggccggtga gcagcagaat caggctgagc ccgaggtgcg
     1981 cctggaaaag gtctggctgc cggcgcaggg cttcccggca ttgctggatg gcctcgtcca
     2041 cccggccttt gcgggccagg acaatgccaa gattgcagcg ggcctgcgcg tcatccggtt
     2101 gccggcggac ggcttcctgg taatgcagaa acgcttcatc aacccggttt ctttcgtaaa
     2161 agacgttgcc caggttgtaa tgggcggtgg cgctgccggg gttcaggcgg agggcctgct
     2221 gatagtgtcg aatggcgtcg tccgtccggc cttttcgccc cagggcaagg ccgaggttga
     2281 cgtgcgtttc ggcgtcatcc ggtctttggc ggatggccgc ctcgaattgg cggatcgcct
     2341 cgtcggtttg gccccggtcg gcgaggattc ggcccaggtt gcggcgcgcc tcggcgaagt
     2401 cctcctgcag gcggatggcc gtctcgaatt gctgctgcgc ttcgttggtt tggcccaggc
     2461 tggccagggc gatcccgaga ttattgtggg cgtgcgcatt atccggctgc aggcgaagcg
     2521 catgctggta ttggcggatc gcttcgttcg tttgtccctg ctgaaggagc gcgagtccga
     2581 ggttgttgtg caggcccgag tcatccggcc gcaggcggag ggcttgctcg tattggcgga
     2641 tggcctcgtc cattttcctc ttcccggcga gggcatcgcc gagcgcctta taagcgacgt
     2701 aattgtcccg ggtgacggct gccgcatgcc ggaacacggt ttcgctctcc tgccaatgtc
     2761 caagctgttg ccgcgtcagc acgaggcaga aggcgagcgc cgccatcgcc caagccaccc
     2821 cggcgccccg ccagcgccgg aaaagcgcat gcgcgcccca aaccaccagc accagcagtc
     2881 ccagcgtcgg gagataagta tagcgatctg ccatcgcctg cccgcccacc tgcaccagtc
     2941 cgatcatggg cacgagcgtt ccgacaaacc acagccatcc caccaacagg aatggcgccc
     3001 gccgccgcca cataaaagcc agccccgaaa cgcccagcat cagcccgccc gaccccaaca
     3061 ccgccgccag cggccaatgc ccgggatatg gatagaacaa ggccaggtcg gtcggccaga
     3121 actgctttcc caggtagcgg ctataggaaa ccagggcgtt cgcgacgcgc gcgctgagtg
     3181 gaaacttccc cgctcccggc atggcgccgg cctgcaccgc ataggtcaca acgctcatca
     3241 ccgccgccag cgcgaagaac gggattttct cccctatcaa tcttccccag ggcacaaccc
     3301 ggcccgaagt cggaactgcc gcgccgcgtt ccgcctcctg ccgggcgcac gacgccattc
     3361 gcatcagcgg ccaatagtcc agcagcaaca ggacgaatgg ccaggtcacc agcgtcgcct
     3421 tggacatcag tcccagggcg aagcagccga gggagagaaa atagaaacgg gccgaagaat
     3481 ggcgggtgcg cgcactggcg ccgtggagcg ctgaaagccg gctatcggca gcgtagcggg
     3541 catagcagat aagcgtcagg aggccgaagc tgccgctgag cacatctttg cgttccgcca
     3601 cccaggcaac cgattcaacc cgcagcggat ggaaagcgaa caacgccgcc accagcaagc
     3661 tgcgcccccg cgctccggtc atctggcgca accccacaaa aaccagcagt gcgttgaggg
     3721 catgcagcag cacgctcgtc aagtgatgcc cccagggcgc cagcccatac acctggccgt
     3781 ccagcatgtg ggatagcacc gtcaccggat gccaattgca gagcagcgga tgccggaaag
     3841 cccatttcac tccctcccag gttaacccgc tccgaacctg cgggttctcc gtgacatagc
     3901 gcgtatcatc gaagctgatg aagtcatggc ccgtggccgg ccaatagagc gccagagttg
     3961 ccagcgccag cgccgcagcc agcagccaca ccgggaagcg caacggaact ttgccggccg
     4021 gggatgccat catttgcgcc ggcgggtatc ggggattgat ctgcgcataa gccgtccctg
     4081 gctgtcgctc aaccgcacga aatcgggaac tttagcataa ccgccttgta gcgggaaccc
     4141 gtgggggccg acaagtctta tctggaggag gcgggcgtcg gggatgcgcc gcgtccaacc
     4201 gggccaagag agcttgcccc cgcccggggt gggttggtta tgctgcccgc acgcaaaagc
     4261 tatgaatgac aatcttgacg agagactcag gttcattttc gggcgaagga gcatccgcgc
     4321 ttattcgagc gcgccggtga gcgaagccac ggtgcaacaa ctcctggccg cggccatggc
     4381 cgcgccgtcg gcggcgggga aggatccctg gcggtttgtc gtcgtccgca accgcggcac
     4441 gctcgcgcag atagccgggg cgctgcccaa tggccgcatg ctggccgagg cggcgctggg
     4501 catcgtcgtg gccggagaac tgccggcggc tcatgatcag caagtgggct atttgctgca
     4561 ggattgctcg gcggccatcg agaacctgct gctgggcgcg catctcctcg gcctgggcgc
     4621 ctgctggctg ggagtgtatc cccgcgaaca acgggtggat gccctccgcc aaatcctggc
     4681 cctgccgcca acggtgattc ccgtaagctg catttccctc gggcatccgg ccgaaaccaa
     4741 gccgccccgc acccgcttca ccccggacta cgtccacttc gagaagtggt aaatggccaa
     4801 ggcgcgcgcc ggggcggtgg cggggtgcct acgccgcctg gcgctgccgc cagttttggc
     4861 ccaactcgag cagcgtttcg gcccggaggc acttggcgag cagcgggaac acatgcttct
     4921 cttcgccgcg gaagtgctcg cgcgaggtcg tgagcgcggc cttgaggagg cgccgggctt
     4981 cagcgcaggt tttagccgta tgcacccggc gcaggcggtc gtctatttcc tgatgctcct
     5041 ggtgcaggct ctggagttca ccgctttgtt cgagaacatg atccaacgcc aggtacgcca
     5101 gctcggtctc ggtgcgggca tgctcttcca gcagcgcttc caccatgccg gccagggtgc
     5161 gaacctcagc cagggtgacc aggctgggca gcgcccgttc gatttgatca aacacgcgca
     5221 gaaaaacgac atgctcggcc agcagtgctt cgataatgct catatctaaa attaacttgg
     5281 ctgaacctgg ccgcaagcgc aaccgggaag tttccggccg gcggcagcgg agggttgaaa
     5341 ccgcgcgccg gtactgagac attgcccggc gcacggccat gcatatacca gacggtttta
     5401 tagacggaaa gaccgcggcc gccaccgcgc tgctttcggc ggcgggggtg gggctggcgc
     5461 tgcggcaggt gcgccggcgg ctgccgccgc ggcgcgtgcc gctgttgggg ctggcggcgg
     5521 cgtttctgtt cgcggcgcag atggtgaact tcccggtggc ggcgggggtg tcggggcacc
     5581 tgattggcgg cgtgctggtg gcggcgctgc tgggcccgag cgcggcggtg gtggtgctga
     5641 cgacggtgct gatcgtgcaa tgctttctgt ttgcggacgg cggggtgctg gcgctcgggg
     5701 ccaacgtgtt caacatggcg ctggtgggcg cggtgggggg ctgcgcgatt taccacggag
     5761 tgcggcgcgg gtggccgggc cggcgcgggc aggtggcggc ggtggcgttt gcgggatggt
     5821 gctccacggt gctggcggcc acatgctgcg ccgggcagct cgcctggtcg ggcaccgtcg
     5881 cctggtcggc cgcgttcccc gcgttgaccg gcacgcacat gatcatcggg gtgggcgagg
     5941 ggctgattag cgcgctggtg ctgctggccg tcctgcgcgc gcgacccgat ttggcctcgg
     6001 agccgggcga cgccgcttct cccgggcgcg ggggcgagtt ggtgcgctat gggttgctgg
     6061 cggcgctggg actggctctg tttgtggcgc ctttcgcgtg cccgtggccg gacgggctgg
     6121 agtccgtggc ggccaggcta ggtttcgagc accgcgccgc cccaccggcg atagcggcgc
     6181 ccgccgccga ttaccaggcg cccggagtct cctgggccgt tggggccacg gcgctggccg
     6241 gagcggcggg gacggtgatc gtctttggcc tggcagggct gctggcctgg tcgctggtgc
     6301 ccaggccgga ggagaaggcc cgggcgggca agccatgatt gaccactgga tcggccacta
     6361 ccatcgcggc ccggacacgg tggtgtcgcg gctgccggcg agcctcaagc tgggggtggc
     6421 gctggcgatc atcgcaggca cggtgctggc gccgccgcgg gcggcgggat ggtatctggc
     6481 cgtggctgcg gcgctggccg gagcggtaat cctcagccgg atgccgatga gatttctcct
     6541 gaaacggctg gcctggctgt cgcccttggt cctgggcgtg gcggtggcaa atgtatttca
     6601 gcctggcggg cgcgggaatt ggccaaccgt ggccgtcaaa agcatgctct gcctggcaac
     6661 aatcatcacc gtgtcgaata ccaccccctt cagccacatc ctgcgcgttt tgcgcgcggt
     6721 gcgcgtgccg gccctgctga tcacgacctt ggcgcttatg caccgctacc tctttgtgct
     6781 ggcggaggag gcggagcgaa tgcgccgcgc ccggaccagc cgcctgttca cgcgcccgcg
     6841 ccgcgcccgc tggcgcctgt tgtccaccgt ggtgggccag cttttcgtgc gcgcctcgga
     6901 gcgggcggag cgcatttacg gcgcaatgtg cgcccgcggc tggaaatgag cgcggccatt
     6961 gaagtcaacg ccctccgcta ccgctaccac gacggcaccg aggcgctgcg gggagtgagt
     7021 ttcacggtgg cgcccggcga atgcgtcggg ctgctcgggc ccaatggctc gggcaaatcc
     7081 accttgttgc tgcacctgaa cggactcctg ccggagcgcc tcgaaccccg cggagccgtg
     7141 cgcattctgg gccagcccat caccccggac aacctcgaaa ccgtgcgccg ccaggtgggg
     7201 ctgctgttcc aggacccgga tgaccaactg ttctgcccga gcgtggcgga ggacgtggcg
     7261 tttggcccgc agcagttggg gcttggcgag gccgaggtga aggcgcgagt ggagcgggcg
     7321 ctggcgcaaa ccggcctggc gggctttggc caccgcgcca cgcaccactt gagccgcggc
     7381 gagaagcgcc gcgcctgcct ggcgggcgtg ctggcgtgcg aaccgggttt gctggtgctg
     7441 gatgaaccca ccagcgacct ggacccacgc ggccggcgtg aattcaaggc cctgctgcaa
     7501 agcatcgccg cgaccaagct gatcgccacg cacgacctgg aattagtggt ggaactatgc
     7561 tcacgcgccg tcgtgctcga ccagggcgtg gtggtggcaa acgggccggt gccggaactg
     7621 ctcaacgacg aagaactcat gctggcgcat ggcctggagc ggcctcatat cctgcggcat
     7681 aagcacccgc attaaagcca ggcctgaaac ggtctattat tccgccaaat ccgtggagga
     7741 acgtttcgcg ggttgccgga aagagagagg gagcccgctg cccggcggca atagagaagc
     7801 ttgttgccgc ttgcaggaag gcagagacgc ggtgcgttgt ggggcggcgg atttaattgc
     7861 ggcggctacg gatgcggctg gggacagcgt tatcgcaggc gggcggaggg gcggatgaag
     7921 gtcaggtcca gcccagccac tggccgatca gttgggtaag gtaggcgaag ccccaggcca
     7981 gcacccccat gtatagccac tggaagaggg gccatttcca ggtgttggtt tcgcgctgaa
     8041 cgatggccac ggtgctcacg cattgcaggg cgaaaacgta aaagacgatg agggtgatgc
     8101 ccgtcagggg ggtatagacg gggctgccgt cgggtttttt ctgcatttgg agggtttggg
     8161 ccaggctgtt catgctggag tgggtggggt cagcgcggcc gacgttatag accgtggaca
     8221 tggtgctgac gaagacttcg cgggcggcaa aagaggtgat gatgccaatg ccgattttcc
     8281 aatcaaaacc cagcggggcg atgacgggtt cgatcagccg ccccagccgc ccggcgaagc
     8341 tctggcgcag gttttcgccg gcggcctggc tgtcgagcgc ggccagttcc gcgcgcagcg
     8401 ccgcggtttg cccggcggtt tgggcgcggg cggcctgggc ttcgagttga gcgcgccggg
     8461 cctcgaggga agcattggcc gccgggttgc gaggataggt ggcaaggaac cacagcagga
     8521 tgttgatgcc cagaatgacg gtgccggcgc ggcgcaggaa gatttgggag cggtcccaca
     8581 tgtggcggat gaccacccgc agcaggggcc gcttgtaggg gggcagttcc atgatgagca
     8641 agggggtgtc gccgcggagc agggtttttt tgaacagcca ggccatgaac agggcgaccc
     8701 cgatgccgag caggtagcag gacagcatgg tcaagcccgg gagtttgaga aagccaaaca
     8761 ccgtcagatt gggaacacag gcggcaatga gcagcgtata aaccggcagc cgggccgagc
     8821 agctcatcaa tggcgccaca aggatggtga ccagccggtc tttggggctt tcgatggtgc
     8881 gggtggccat aatgccgggg atggcgcagg caaaggagct gagcatggga atgaagctct
     8941 tgccgtgcag gccgactttg ctcatgaacc ggtccatcag aaaggcggcg cgcgccatgt
     9001 agccagtgtc ttcgaggaag ccgatgaaca gaaacagcaa caggatttgc ggcatgaaca
     9061 ccaccaccgc gcccacaccg gcgataaccc catccaccag caagttgttg aggtcgccgg
     9121 ggggcagggc ggcgcccacg gtgcggccaa accaatccac gccggtttgg agggcatcca
     9181 tggggatgcg cgcgaaggtg aagatgccct gaaacatcaa ggtcatgagg gccaggaaga
     9241 tgaccgtccc ccagagcttg tgggtcagca cgtggtcaag ccggtcgctg aaggtttcgc
     9301 cgggcggcac cagttcggtc gtggccgcct gctcaattgc cgccacggcc tggtagcggt
     9361 tctcaatgcc gatggtgcgc cagtcaaccc cggcggtttc gaggcgctgg cgggcggcgg
     9421 agacggcatc cagaaccggc gccgggtaat gttccaggct cgaagcgagg gccttttcgt
     9481 tgctgagaat caggagcgcc tcggccgccg cctgcgcgcg ccgttcctga aacgtttggg
     9541 ccagcagcgc ggcgagggcg tcaagctccg cctgcagcac gggaggcagc tccgggaagc
     9601 ggcgggtccc ggcgggggcc ggacggtcaa gcgccgcctg gatggcgcgc cgcagttcgg
     9661 gcatcccctg gccggtgttg gcgaccagcg ggcataccgg cacgccgagt tcggcggcca
     9721 gcttatcggg ctgaatgcgg tgcccgattt tctccgccac gtccaccatg ttcagcgcaa
     9781 ccagggtcgg gtagcccagt tcgatcacct gggtggcgta gtagaggttg cgttgcaaat
     9841 tggaggcatc caccacgagg acgattaccg ttggggcggg cagttcgggc agccggttga
     9901 gcaggacatc gcgggagatt tgctcgtcaa gggaatgcgg gctaaggctg tagctgccgg
     9961 gcaaatccag cacgcggacg ttggcgcccg gcggcactcc caacaggcgc ccctctttgc
    10021 gctccacggt cacgccggca taattgccga ccttggcacg caagccggtc agggcgttga
    10081 aaagcgtcgt tttaccggag ttcggattgc cggttaacac cacgtagcgg gggcccgggg
    10141 ggggcggggt ggggctggcg gacccggcgc gttcctccgg ggtgcgggaa gctgctcctc
    10201 cgggtttacg cattcggttg caccaggatt tgctccgctt cgtgcttgcg cagagttaaa
    10261 tggtagcccc gaaccttgat atccaccggg tcgcccagtg gggcaaagcg cactaattct
    10321 accggggtgc ccaccagcaa acccatttcc atcaggcggg ggcggctttc cgggggcaga
    10381 ttgatttcga ccacaacggc ccggccgccc aggggcagcg aagtcagggg caggggagtt
    10441 ggcggcataa cgacgcaacc atcaagcgcg cgcgggccgg gacggcctct ccggagaggg
    10501 cgccggcttg gcggattcag gccgggatgg cgctggctgg ttcacagccg ataaattagg
    10561 gcaagagcgc cccggctgca agcgtggcag cggcgcccga tgccgcgcgc ggcgccgccg
    10621 tgctgcggtc gcgcaatcgc cgcaccggcg cgagcgcgcc ataaaccggg gcaatctccg
    10681 gccggaggac ggtttttaag cttctcaacc ggcggtgggc taaggcacct tgaggcgcac
    10741 agctatgtat cgggaccaaa aagtcgtggt ggtgatgccg gcctacaatg cggccaaaac
    10801 gctgcgtcag acttatgcgg aagtgcgcga gcagggggtg gtggaccaaa ttattctggt
    10861 ggacgataac agccgggacg agacggtggc ggtcgcgcgg cagttggaag gagtgctcgt
    10921 gcatgtccac gaggtgaata aaggctacgg cgggaatcaa aagacctgct accggctggc
    10981 cttggcggcg gcggcggaca tcgttatcat gattcacccg gactaccagt acacgccccg
    11041 gctgattccc gcgatggtct ccatcatcgg caacggcttg cacccgtgcg tcctgggaag
    11101 ccggattctg ggaggctacg cgctgcgggg gggcatgccc atttggaaat acgtggccaa
    11161 tcggggcctg acgctggtgg agaatattct gctgggggcc aaattatccg aataccacac
    11221 cggctaccgc gccttctcgc gggcgatcct ggagaagctg gatttgcagg gcaactcgga
    11281 tgatttcgtg ttcgacaacc agatgctggc gcaaatcctg tggcatggct tcaccattgc
    11341 cgaggtgagc tgcccgacca aatactttcc cgaagcctcg tccatcaacc tgcggcggag
    11401 catcaaatac gggtgcggct gcctggcgac gggcctgcaa taccgcctga gcaagatggg
    11461 agtgctccgt tcccggcttt tcccccggat ggcgggaaaa gcccagggcc ggagcgcgaa
    11521 ggcgccgccc ggatagcccg gcaaaaggcg cgtcgcggag ggggctactt cgccttggcc
    11581 aaacgcagcg ccaggcgtga tttcttgcgg ttgacggtgg cgcggggcag cacgcccgtc
    11641 ttggcggcct tgtccaaagc ggagctgagc gtgcgcaatt cccgggcggc ctcatccttt
    11701 ttgccgctgc tgagttgttt caggtaggcg ccctcgagcg tatggaggcg agatttggcg
    11761 ctgcgattgc gcagattccg ccgggcgctg ctgcgcatgc ggcgttcggc tgactttgta
    11821 ttcggcataa atcaaatcca atcagtgatt caaagcgccc caccttaacg gaataacggg
    11881 cattgtcaac gccggggtca gggtctttgg ttttgtaaat cataagccgg ggcggttgag
    11941 gccgtggatg gggcggcggt taagcgcttc ggagcgggtg cttgcccatg cgcccagggc
    12001 ccgggctgtg ggaaacagat ggggctggtt gctttgcgcg gatggagatc gtcgccgccc
    12061 ctttgcccaa tcttccgggc cagtttgcat tggccgggca agggagcggc ggtgatgagc
    12121 ttgccctcca agaccggcag gccttcccgc agacaatccg ggcaggcgcg caggtgcttt
    12181 tctcagtgcc gggcgaacgg ctcccgagcg ccgcgtgagg gcacgcggcc tacaggaaat
    12241 gg
//
LOCUS       DAOPQG010000102        12226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6012_length_12226_cov_14.400049, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000102 DAOPQG010000000
VERSION     DAOPQG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6012_length_12226_cov_14.400049"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            512..2077
                     /locus_tag="PKI07_10925"
     CDS             512..2077
                     /locus_tag="PKI07_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOC51603.1"
                     /translation="MPATKESDSLDARFYRAADAFFSAHSDLALTYDDVTLATLYSEI
                     LPRDTQLSTRLAEGLELNIPIISADMDTVTESRMAIALALHGGLGLIHYNMPERQQLS
                     EVSRVKNHVHGLIQDPIKTSPGQLIGEVLELIAKKRFAFSTFPVVDDQGKLIGLLSGS
                     VVKQRYARRAVAELLTPRSQVHTLRHQKLGRDPIARADSFFAEHPGIHKLLVVDEEDR
                     LQGLFTLSDVERIIQERSAQFKPARDARFRLLCGAAVSATRNAFGELDRERILAHVGA
                     LVERGLDVVAVSTAHGHSRGVGDAVRMLRGAFPGLPIIAGNVTSAAGVEYLADCGANV
                     VKVGQGPGSICTTRIVAGVGIPQLTALYLASRAARQKRVTILADGGITKSGDIVKALT
                     LAQAVVCGGVLAGCSEAPGEVMEIGGKLYKQYRGMGSWAAMKAGAAARYGHTNDPARK
                     IAPEGIEALKEVSGALDRVLAQLIGGIQSGMGYLGAANLAQLREKARYIRVSPAGQRE
                     AVPHDVVELKPGS"
     gene            complement(2092..2316)
                     /locus_tag="PKI07_10930"
     CDS             complement(2092..2316)
                     /locus_tag="PKI07_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51604.1"
                     /translation="MPIPHLIFTYVVVLLAGLTGAALYSEFRRRSFEPTGSEDRIFRC
                     RHCGYVYTDDPDVARSRCSQCGQMNEAIKF"
     gene            complement(2341..3138)
                     /locus_tag="PKI07_10935"
     CDS             complement(2341..3138)
                     /locus_tag="PKI07_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOC51605.1"
                     /translation="MTPAPAASRSWTRRRWWGVAALVFLFQLALIFWLGETAPPTPHP
                     AAPGLTLRLAGSAAAELLALRDPTLFVLPRPQVISTPAWFKAPHPDADVFAWPEPANP
                     PALSIGQPGTALSQRAESNLFPLQQALARFEPHPDLPEAAPLPMGADQSTLQLRGALA
                     QRRLLAPLDLRSWTHRDILSNSVVQLLVDAEGMPRSITLLAGSGSPEADRYALALARG
                     ARFEPLRPNPGDPASVPAAHLSWGRMVFRWHTQPLPPTNTPAAGPQP"
     gene            complement(3135..3575)
                     /locus_tag="PKI07_10940"
     CDS             complement(3135..3575)
                     /locus_tag="PKI07_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOC51606.1"
                     /translation="MKFPRQATIFRRQLDAAPFAAMFFLLVLFMMLASLVYTPGARLE
                     LRLPEGAGLPGADRPSVSVAIDADGRLYYENQWIQEDELRRRLQAAVAKAGEPLTLIV
                     QADKAVSYERCLRLALLAREAGMTDALLATLPGPYAVPPLRSPP"
     gene            complement(3572..4207)
                     /locus_tag="PKI07_10945"
     CDS             complement(3572..4207)
                     /locus_tag="PKI07_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOC51607.1"
                     /translation="MPTLPVMLSHGGPVLWLLLIISAVAVAVFIERFLHCHRAQINST
                     EFLNGIRTVLKRDNPVEALSICDATPGPVARLVRIAILHREEGRERVREALEEAGAVE
                     VPRLEEKLSLLATIAQLAPLIGLLGTMLGFIQTFSRMQEAGLNAHVGLLSSGIWQALV
                     CAAAGLAVSIPAHAAYNYLVIRVNAIVLDMERSATEIVRIVTETAKPPPAP"
     gene            4322..4702
                     /locus_tag="PKI07_10950"
     CDS             4322..4702
                     /locus_tag="PKI07_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HOC51608.1"
                     /translation="MEQPDQLRALFQMQKALNERIGVRTECMNTEEKTRWLLNYCRAM
                     SQEIAELTDSVPWKWWAKYQKFDEQNARVEVVDLFHFLISLAQVLGMSADDVFAAYVR
                     KNEVNFKRQDAGYTAKDEHDSKHI"
     gene            4754..5404
                     /gene="hpt"
                     /locus_tag="PKI07_10955"
     CDS             4754..5404
                     /gene="hpt"
                     /locus_tag="PKI07_10955"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOC51609.1"
                     /translation="MSPSFPQTPARHGVAVPRRWRREVERILITDAQIARRIRTLARA
                     IARDFAGRELVVVSLLNGTVMFLADLIRNLSLPLRLDFIGVSSYGAGTESGELVFTKE
                     LRLSVRGRDVLLVDDILDTGKTLSRVRARLRALRPRRIRICVLLNKAARRVEPVAADY
                     VGFEIPDEFVVGYGLDFAERYRNLPFVGVLYPHVYQQGCRRARPARPRAARARRRA"
     gene            complement(5423..5498)
                     /locus_tag="PKI07_10960"
     tRNA            complement(5423..5498)
                     /locus_tag="PKI07_10960"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5463..5465),aa:Val,seq:tac)
     gene            complement(5587..7080)
                     /locus_tag="PKI07_10965"
     CDS             complement(5587..7080)
                     /locus_tag="PKI07_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC51610.1"
                     /translation="MHPLAQLDHRYLWHPFTQMRDWLKREPILIVRGRGARVRDARGR
                     EYLDANASIWTNLHGHHHPRINAAIRRQLDRIAHSSALGLANAPAARLGEQLVQAANP
                     PRRGPAARLLRQPVLGKVFFSDNGSTAMEVALKLAYEFTRRSGRDARPRFLTLDGAYH
                     GDTVGAVSLGHIDLFHKAYSGLLFPTDKVPAPYCYRCAFNRARPERADARDYRQCRWE
                     CLDALERKFAAQARRGNPYAAFVFEPLMQGAAGMIPQPAGWLRRAAAIARAHGALLIA
                     DEVMTGFGRTAPRESGLSGAAQPRRKTGPAETPDLPLFACHHEGVRPDFMALAKGLSG
                     GYLPMAATLTTQAVFDAFLGEYEEFKTFFHGHSFTANQLGAAAGLASLAILRSTASIR
                     ARIRLAQTLRAGLETLWRSPHAGDIRQVGLIAGIELVRDWRTRAPFDLRERVGIRVCE
                     TLARLGVLTRPIGNVIVLMPPYCTTPAEARRIIAALGQALDDLRQSL"
     gene            complement(7091..8287)
                     /locus_tag="PKI07_10970"
     CDS             complement(7091..8287)
                     /locus_tag="PKI07_10970"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HOC51611.1"
                     /translation="MMNDFDGEMARRLAALRADGLWRELRRVETPQSPRLRIGGQTLL
                     NFASNDYLGLANDPAVREAAIRAVEQYGAGAGASRLVCGSLAPHHALDEALAAFKGVE
                     AALSFSSGYASALGALGALLGRGDFVILDQRVHASIVDAARLCGAKLRVFNHNDLDSL
                     EARLKWVRAQASARKARALVVTESVFSMDGDHAPLRELAALKERYGAWLMVDEAHAVG
                     LYGARGRGLAAALGVSERIEVQMGTLGKAMGAAGGYLCGSRALVDCLINRARPFLFST
                     APVPAAVAAAAAGVRLAQSRTGEERRARLWQNVARLLPAAPARAPTDRSAIIPIRIGD
                     EARALAIADALRAREIFLPAIRYPTVARGAARLRLTVTAAHTPADLDQLQAALDALGL
                     ELDPGS"
     gene            complement(8415..9752)
                     /locus_tag="PKI07_10975"
     CDS             complement(8415..9752)
                     /locus_tag="PKI07_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC51612.1"
                     /translation="MRAGGWKTAERKRRRHARPRPGREARPGSLLLRARVVLPVARPP
                     LADGAVLISGRRIAAVGRWRDLRAAKPGQVADLGEVALLPGLVNAHAHLDYTHMAGQL
                     PPPRGFSDWLKLITETKSGWSLADYAASWQAGAQMLLRTGTTTVADIEAVPQLLPEAW
                     AATPLRVFSFLELIGIKGAAQPRAVVREAAARLGALRHRRCRVGLSPHAPYSTVPELI
                     RLSVRAARRRNWRLTMHVAESAPEYEMFARGRGELYDWLRRSGRDMSDCGRGSPVRHL
                     HRCGALSERLLVVHANYLARPDVALLARHRVPVVHCPRSHDYFRHDPFPWRRLLRAGV
                     NVCLGTDSLASVYQARRERVELSLFAEMRALARSAPGLSARRILRMATVNGARALGME
                     GQLGELGEGAGADLVALPFAGKTADAYEGVLHHEGAVSASMIDGQWAIAPSSD"
     gene            complement(9777..10130)
                     /gene="raiA"
                     /locus_tag="PKI07_10980"
     CDS             complement(9777..10130)
                     /gene="raiA"
                     /locus_tag="PKI07_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOC51613.1"
                     /translation="MNLILATHNLTLTRAIEAHIVNRVDKLARLDRFAINARVTLAHD
                     KTKAPERQFSCSVRLEVPGPDLFAEDTQGDLYAAIDLVVKKIEQQIRKRHNKYKARKH
                     RVAERGKRLRQDTGF"
     gene            10258..11079
                     /locus_tag="PKI07_10985"
     CDS             10258..11079
                     /locus_tag="PKI07_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019543431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOC51614.1"
                     /translation="MPTKVIAVANQKGGVGKTTTAVNLAACVAAVGRRVLLFDLDPQA
                     NATSGVGLEKTPGASAYLALLGQGRLQDKVRNTAFARLAVIPGEVDLCGADLELARAD
                     NHLQRVRLALQPLREERRFDVIFVDCPPSLGVLTLNALAAADGVLVPLQCEYYALEGL
                     SMLNRIMTQLHDTGVNPRLELTGVVMTMYDSRTKLSQQVVGEVRQHFGERVFETLIPR
                     STRLAEAPSYGQPIIHYDKYSAGSAAYELLAQEFLARLAQDREPRQPGPPRSENQ"
     gene            11114..12175
                     /locus_tag="PKI07_10990"
     CDS             11114..12175
                     /locus_tag="PKI07_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51615.1"
                     /translation="MSNKVKWGVIGCGGIAARRTIPELKKLAANAELVSVMDTNAARA
                     REVAAQFGVPHACATEAELLAQDVQAVYIASPPNAHCRQTVQAARAGKHILCEKPIAV
                     SVAEVDQMEAACAQAGVKFMLAWCMRQNVYHQQARELVQSGALGRMVMGRAQLTCWYP
                     PIPGAWRQDIALSHGGALLDMGTHCLDLLEWIFGSPIIEVTGFTDLLIHAYATPIEDT
                     ATILVRFRNGAHGIIDNYFNIPDAAAQNALELFGTKGSIIARGTIGQEPTGQMFSILQ
                     AQETGYDAAQTRDSQPNRREYKLEGRGIYGQMITLFSDCILQDRPPPASLADGRHSVK
                     VVNAIYQAARERRVVEVAA"
BASE COUNT         2108 a         4130 c         4119 g         1869 t
ORIGIN      
        1 ccggctgcgc acggcgggcg aagtttgcca gtagattgag gcgggctgga gttgcaggcc
       61 ttgcgaaaac gggtccaggc ggccagccca ccgtaggggg gtgggctgcc tcgtcggggc
      121 gctcaatcgc cgccatcccg cccaaggtaa tcagttcaac agggcaacga cctggccggt
      181 ctcctccacg atttggcgca ggcaatcgcc aacaggccgt cccgcgtcca acgggtgctg
      241 ttggtccggc aaggtatcaa accaccgatg ttcatccttt cgggctgttc gcacgcgcag
      301 cgccgggttg ctcgcagagt cggccggctg ttttgaagcg atagacgctt tcatccccgc
      361 ctcccaaccc gaaaatcggc tttgggttta gcaaaatctt ttccccctca tctaccgaaa
      421 ctcaggcccg ccaacaaaag gccctgctca gagcggagtc ggaatgagct tttggcttgt
      481 cagggagttg ccggggatgg catttttcga catgccagca accaaagagt ccgatagcct
      541 tgatgcccga ttttaccggg cggccgacgc tttcttctcc gcccatagcg acctggccct
      601 gacctatgac gatgtgacgc tggcgacgtt gtattcggag attctgcccc gggatacgca
      661 gctcagcacg cgactggccg aagggttgga actgaacatt cccatcatct cggcggatat
      721 ggatacggtg accgagtcgc gcatggccat cgcgctggcg ctccacggcg gattggggct
      781 gattcattac aacatgcccg agcggcagca gctttccgaa gtcagccggg tgaagaacca
      841 cgtgcatggg ctgattcagg atccgatcaa gacgtcgccc gggcagttga ttggcgaggt
      901 gctggagttg attgcgaaaa agcggtttgc cttcagcacg ttcccggtgg tggacgacca
      961 gggcaaactg atcggcctgc tgtcgggcag cgttgtgaag cagcgctacg cccggcgcgc
     1021 cgtagccgaa ttgctcacgc cgcgcagcca ggtgcatacc cttcgccacc agaagctggg
     1081 ccgggatccc atcgcccggg ccgacagttt ttttgccgag cacccgggca ttcataagct
     1141 gctggtggtg gatgaggagg atcgcctgca agggctgttc acgctgagcg acgtggagcg
     1201 gatcatccag gagcggagcg cgcagttcaa gccggcgcgc gacgcgcggt tccgcctgct
     1261 ctgcggggcg gcggtttccg cgacgcgcaa cgcctttggc gagctggacc gcgagcgcat
     1321 cctggcgcac gttggcgcgc tggtggagcg ggggctggat gtggtggccg tctcgaccgc
     1381 gcatggccac agccgcggcg tgggggatgc ggtgcggatg ctgcgcggcg cttttcccgg
     1441 gctgccgatt attgcgggca acgtgaccag tgccgcgggg gtggagtacc tggccgattg
     1501 cggcgcgaat gtggttaagg tggggcaggg gccgggctcg atctgcacca cgcgcatcgt
     1561 cgccggcgtc ggcatcccgc aactgacggc gctgtacctg gcctcgcggg ccgcccggca
     1621 aaagcgggtg acgattctgg ccgacggcgg cattaccaaa tcgggggata ttgtaaaggc
     1681 gctgacgctg gcccaggcgg tcgtctgcgg cggcgtgctg gccggctgct cggaagcgcc
     1741 cggcgaggtg atggaaattg gcggcaaact ctacaagcaa tatcggggca tgggcagttg
     1801 ggcggccatg aaagccgggg cggcggcgcg gtatggccac acgaacgatc ccgcccgcaa
     1861 gatcgccccg gagggcatcg aggcgctgaa ggaagtcagc ggcgcgcttg atcgcgtgct
     1921 cgcgcaactg attgggggga ttcaatccgg catgggctac ctcggcgcgg ccaacctggc
     1981 ccaactccgc gaaaaggcgc gctatatccg cgtcagcccc gccggccagc gggaggccgt
     2041 gccgcatgat gtggtggagt tgaagccggg gagttgagcc gccgcgggcg ctcagaattt
     2101 gatcgcttca ttcatctgcc cgcactgcga gcagcgcgag cgggccacgt ccgggtcgtc
     2161 cgtataaacg tagccacagt ggcggcagcg aaaaatgcgg tcctcgctgc ccgtcggctc
     2221 gaagctccgc cggcgaaact cggaatagag ggcggcgccc gtcaaaccgg ccagaaggac
     2281 aaccacgtaa gtgaagatca ggtgcggaat cggcatggtg aaggcgcgag gaagtgccgg
     2341 tcagggttgc gggccggccg ccggggtgtt cgtgggcggc agcggctggg tgtgccaccg
     2401 gaagaccatc cggccccagc tcagatgcgc ggctggcacg gaggcaggat cgcccgggtt
     2461 ggggcggagc ggctcgaagc gcgcgccccg ggccagggcc aaggcgtaac ggtcagcttc
     2521 cggggaaccg ctgccggcca gcaaggtgat ggaacgcggc atcccttcgg cgtccaccag
     2581 gagttgcacg acgctattgc tcaaaatgtc ccgatgggtc caggaacgca aatccagcgg
     2641 cgccaatagc cgccgttggg caagggcgcc gcgcagttgc agggtggatt gatcagcccc
     2701 catcggcaga ggggccgcct cgggcaggtc cgggtggggc tcgaatcgcg ccaacgcctg
     2761 ctgaagcggg aagaggttgc tctcggcccg ttggctgaga gccgtgccag gttggccgat
     2821 gctcaacgcg ggaggatttg ccggctcggg ccaggcgaag acgtccgcgt cgggatgcgg
     2881 ggccttgaac caggccggcg tcgaaatcac ctgcgggcgc ggcaggacaa acaaggtggg
     2941 gtcgcgcagc gccagcaatt cggcggcggc gcttccggcc agccgcagcg tcaggccggg
     3001 cgcggccggg tgaggggtcg gaggcgcggt ctcgcccagc cagaaaatca gggcgagttg
     3061 gaacaggaat accagcgccg ccacgcccca ccagcggcga cgcgtccagg agcgcgatgc
     3121 ggccggcgcc ggggtcatgg cggcgagcgg agcggcggga cggcgtaagg ccccggcagc
     3181 gtggccagca gggcgtcggt catgccggct tcgcgcgcca gcagcgccag gcgcaggcag
     3241 cgttcataac tcaccgcctt gtcggcttgc acaatcaacg tcaggggttc gccggccttc
     3301 gccaccgccg cctgcagccg gcggcgcagc tcgtcttcct ggatccactg gttttcgtaa
     3361 taaagccgcc cgtcggcgtc aatggcgact gataccgacg gccggtcggc gcccggcaaa
     3421 cccgcgccct ccggcagccg caattccagg cgcgctccgg gcgtataaac cagcgacgcc
     3481 agcatcatga atagcaccag cagaaagaac atcgcggcaa acggcgcggc gtccaactgc
     3541 cgccggaaaa tggtggcctg acgggggaac ttcatggcgc cgggggcggc ttggccgttt
     3601 cggtgacgat gcgcacgatc tcggtggcgg agcgttccat gtccagcacg atggcattga
     3661 cgcggatcac caggtagtta taggccgcat gcgcggggat ggagaccgcc aggcccgccg
     3721 ccgcgcagac cagcgcctgc cagattccgc tggagagcag gcccacgtgc gcgttcagcc
     3781 cggcttcctg catccggcta aaggtctgga tgaaccccag catggttccc agcaggccga
     3841 tcagcggcgc cagttgcgca atcgtcgcca gcaggctgag tttctcctcc agccgcggca
     3901 cttcgaccgc cccggcttcc tccagcgcct cgcgcacgcg ttcccggccc tcttcccgat
     3961 gcaaaatggc aatccggacc agccgggcga ccgggccggg cgtggcatcg caaatggaca
     4021 gcgcctcgac cgggttatcg cgcttcagga cggtgcggat gccgttgagg aactcggtcg
     4081 aattgatttg cgcgcggtgg caatgcagaa agcgctcaat gaagacggcc acggccacgg
     4141 cgctgatgat caggagcagc catagcacgg gtccgccgtg ggataacatt accggcaatg
     4201 ttggcatggc tctctttata gggactcgga cggaaggttc aaagtgcaaa gttcaaagcg
     4261 cggcggcgcg ggcgcgcggt ttcgccttgc ttcccgcccg cgctcgtcga atcctcccgg
     4321 catggaacaa ccagaccagc ttcgcgcgct cttccagatg cagaaagcgt tgaacgagcg
     4381 tattggcgtc cgcaccgagt gcatgaacac cgaggagaag accaggtggc tgctcaatta
     4441 ttgccgggcc atgtcccagg agattgccga actgaccgac agcgtgccgt ggaaatggtg
     4501 ggccaaatac cagaagtttg acgagcagaa tgcccgcgtc gaggtcgttg acctgttcca
     4561 tttcctgatc agcctggccc aggtgctggg aatgagcgcg gacgacgtgt tcgcggcgta
     4621 tgtgcgcaaa aatgaggtca actttaaacg gcaggacgcc ggctataccg ccaaggacga
     4681 gcatgattca aagcacatct agcccggccc ccgcgcgccg ccgcccggaa gccgcccgcc
     4741 aagcgccccg cccatgagcc cgtcgttccc ccaaaccccg gcccgccacg gcgtcgccgt
     4801 tccccggcgc tggcgccgcg aagtcgaacg catcctgatc accgacgccc aaatcgcgcg
     4861 ccgcatccga acgctggccc gcgccatcgc gcgcgatttc gccgggcgcg agttggtggt
     4921 cgtttcgctg ctcaacggga cggtgatgtt cctggcggat ctcattcgga atctctcgct
     4981 gcccctgcgg ctggatttca tcggcgtctc cagctacggc gcgggcaccg agtccggcga
     5041 actggtcttc accaaggaac tgcggctgag cgtccgcgga cgcgatgtgc tgctggtgga
     5101 tgatattctg gacaccggca aaacgctttc ccgggtgcgg gcccggctcc gcgccctccg
     5161 cccgcgccgg atccggatct gcgtcctgct gaacaaagcc gcccgccgcg tggaaccggt
     5221 cgcggcggat tatgtgggct tcgagatccc ggacgagttt gtggtgggct acggattgga
     5281 cttcgcggag cggtatcgca acctgccgtt tgtgggcgtc ttatacccgc atgtttatca
     5341 gcaaggctgc cggcgagccc ggcccgcccg cccccgcgca gcgcgcgcgc gacgccgggc
     5401 ctgactgttc cggcgggaaa gatggtgggc gctgagggat tcgaaccccc gacctactcg
     5461 gtgtaaacga gacgcgctaa ccactgcgcc aagcgcccag cccggtcatc ctatgccgaa
     5521 tggcgtgcgt atgacaagcg ctggtcttcc gggtttcccc gcggagcgcc ggaaagggcc
     5581 ggcgtttcac aatgattggc gcaggtcatc cagggcctgg ccgagggcgg cgatgatgcg
     5641 ccgggcttcg gcgggtgtgg tgcagtaggg cggcatgagg acgatgacat tgccaatcgg
     5701 gcgcgtcagc acgccgagcc gggccagggt ttcgcacaca cgaatgccca cgcgttcgcg
     5761 aaggtcgaag ggcgcgcgcg tgcgccagtc gcgaaccagc tcgatgccgg cgattaagcc
     5821 cacctgccgg atgtcgccgg cgtgcggact gcgccagagt gtctcgagtc cagcgcgcag
     5881 ggtttgcgcc aggcgaatcc gggcgcggat ggaggcggtg gagcgtaaga tagccaggct
     5941 ggccaggccg gccgccgccc cgagttgatt ggccgtgaag ctgtgcccgt ggaaaaaagt
     6001 cttgaattcc tcgtattcgc cgaggaaagc gtcgaaaacc gcctgggtgg tcagcgtggc
     6061 ggccatcggc aggtagccgc cgctcaggcc cttggcgaga gccatgaagt ccggccgcac
     6121 cccttcgtgg tggcaggcaa acagcgggag atcgggtgtc tccgcggggc cggtctttcg
     6181 ccggggttgc gccgcgccgg aaagcccgct ttcccggggc gcggtgcggc cgaagccggt
     6241 catgacttcg tcggcaatca gcagagcgcc atgcgcgcgc gctatcgccg cggcgcggcg
     6301 cagccagccc gccggctgcg ggatcatgcc cgccgcgccc tgcatcagcg gctcgaagac
     6361 gaaagcggcg taaggattgc cccgccgggc ctgggccgca aacttgcgtt ccagggcgtc
     6421 aaggcactcc caccggcact ggcggtaatc gcgggcgtcg gcacgctcgg gccgggctcg
     6481 gttaaaggcg caccggtagc aatacggggc cgggaccttg tccgtcggaa agagcaggcc
     6541 ggagtaagcc ttgtggaaca agtcaatgtg ccccagggaa accgccccga cggtgtcgcc
     6601 atgatacgcc ccgtccagcg tcagaaaccg cggccgggcg tcgcggcccg agcggcgggt
     6661 gaactcatag gcgagcttga gggcaacttc catcgccgtc gagccgttgt cggagaagaa
     6721 gaccttgccc agcacgggct gccggagcag cctggccgcc gggccccgcc gtggcgggtt
     6781 ggccgcttga accaattgct cccccagtcg cgcggccggc gcattggcga ggcccagcgc
     6841 cgagctatgg gcaatccggt cgagctggcg gcgaatggcg gcgttgatcc ggggatgatg
     6901 gtgcccgtgc aggttggtcc agatggacgc attggcgtcc aggtattcgc gcccgcgcgc
     6961 atcgcgcacc cgcgcgcccc ggccgcgcac gatcaggatg ggctcgcgct tgagccagtc
     7021 gcgcatctgc gtaaagggat gccataaata acggtgatcg agttgggcca gagggtgcat
     7081 aaaggcaagg tcaggaaccc gggtctaatt caaggccaag cgcgtcgagc gccgcctgca
     7141 gttgatcaag gtccgccggc gtgtgggcgg cggtcacggt caggcgaagg cgcgcggcgc
     7201 cgcgcgcgac cgtcggataa cggatggccg ggagaaaaat ctcccgcgcg cgcagcgcgt
     7261 cagcgatcgc caacgcccgc gcttcatcgc cgatccgaat cgggataatg gcgctccggt
     7321 ccgtgggcgc gcgggcgggg gccgccggca ggaggcgggc gacattctgc cacagcctgg
     7381 cccggcgctc ctcgccggtg cgcgattgcg ccaggcgcac gcccgcggcg gcagccgcga
     7441 cggcggcagg caccggcgcg gtcgaaaaga ggaagggccg ggcgcggtta atcaaacaat
     7501 ccaccagggc ccgggagccg cagaggtagc ccccggccgc ccccatggcc ttgcctagcg
     7561 tgcccatctg gacttcgatg cgctcgctga cgccgagcgc cgccgccagg ccgcggcccc
     7621 gcgcgccgta aagccccacg gcatgcgctt cgtccaccat cagccacgcg ccgtatcgct
     7681 ccttcaaggc agccagctcc cgcagcggcg cgtgatcgcc atccatcgag aagacgctct
     7741 cggtgaccac cagcgcgcgc gccttgcggg ccgaggcctg cgcgcgaacc catttgagcc
     7801 gcgcttccag gctgtccaga tcgttgtggt taaacacccg gagcttcgcg ccgcaaagcc
     7861 gggcggcatc cacgatgctg gcatgcaccc gctggtcgag gatgacgaag tccccccggc
     7921 ccagcagcgc gcccagggcg ccgagggcgc tggcgtagcc ggaagaaaag ctcaaggccg
     7981 cctccacgcc cttgaaggcc gccagcgcct cgtccagcgc gtgatgaggc gccagcgaac
     8041 cacagaccag gcgcgacgcc ccggcgcccg cgccatattg ctcgacggcg cgaatggccg
     8101 cctcccgcac cgcggggtca ttggccagac ccaggtaatc gttcgaggcg aagttcagca
     8161 gagtctggcc gccgatccgg aggcggggcg attggggcgt ctccacccgg cgcaactcgc
     8221 gccacagccc atccgcgcgc agcgccgcga gccggcgggc catttccccg tcaaaatcgt
     8281 tcatcatcgc aaagcccccc gttgccgggc cgccggcgtg cttcccgccg cggaggaaac
     8341 cggcaagcgc cccggctggc ggacgttgcc ggccggcgtt tcgccgcgca gctccaggat
     8401 tccgcaacgc cccatcagtc ggaagacggt gcgatggccc actgcccgtc aatcatgctg
     8461 gcgctcaccg ctccctcatg atgcagcacc ccttcatagg catccgctgt tttcccggca
     8521 aacggcagcg ccacgagatc cgccccggct ccttccccca gttcccccag ttgcccctcc
     8581 attcccaggg cgcgggcgcc gttcaccgtg gccatgcgca aaatccggcg cgccgaaagc
     8641 ccgggcgcgc tccgcgccag cgcgcgcatt tccgcgaaca ggcttaactc cacccgttcg
     8701 cgccgggcct ggtaaacgct ggccaggctg tcggtgccca ggcagacatt gacgcccgcc
     8761 cgcagcaggc gccgccacgg aaacgggtca tggcggaagt agtcatgact gcgcgggcaa
     8821 tgcaccaccg gcacccggtg ccgcgccagc agcgccacgt cgggccgcgc caggtagttc
     8881 gcatgcacga ccagcaaccg ctcgctgagc gcgccgcagc ggtgcagatg ccgcacgggc
     8941 gagccccggc cgcagtccga catgtcgcgc ccgctgcgcc ggagccagtc gtacagctcg
     9001 ccccgccccc gcgcgaacat ttcatattcc ggcgcggatt ccgccacgtg catcgtcagg
     9061 cgccagttgc gccggcgcgc cgcgcggacg ctcagccgga tcaactcggg cacggtggag
     9121 tagggcgcat ggggcgaaag gccgacgcgg cagcggcgat gccgcagtgc gcccagccgg
     9181 gcggcggcct cccgcaccac ggcgcgcggt tgcgccgcgc ccttgatgcc gatcagctcc
     9241 agaaaggaaa aaacgcgcag cggcgtcgcc gcccaggcct ccggcagcag ttgcggcacc
     9301 gcctcgatgt cggccacggt cgtcgtgccg gtgcgcagca gcatttgcgc gcccgcctgc
     9361 caggacgccg cgtaatccgc gaggctccag ccggacttgg tttcggtgat cagcttcagc
     9421 cagtcgctga acccccgggg gggcggcaac tggccggcca tgtgggtgta atccaggtgc
     9481 gcgtgcgcgt tgacgaggcc gggcaggagc gccacttcgc ccaggtccgc aacctgtccg
     9541 ggcttcgccg cccgcaggtc gcgccagcgg cccaccgcgg cgatccgccg cccggaaatc
     9601 agcaccgcgc catcagccag cggcggccgg gccacgggca ggacgacgcg cgcgcgcagc
     9661 agcagggagc cggggcgcgc ctcacgcccg ggccgcgggc gggcgtggcg gcggcgcttg
     9721 cgttccgccg tcttccatcc gccggccctc accgcgcgtt tcctctccgc gtgaactcag
     9781 aacccggtgt cctggcgcag gcgcttgccg cgctccgcca cccggtgttt gcgggccttg
     9841 tatttattat gccgcttgcg gatttgttgc tcgatctttt tgacgacgag gtcaatcgcc
     9901 gcgtagaggt cgccctgggt gtcttccgcg aacagatcgg ggccgggcac ttccaaacgc
     9961 accgaacagg agaactgccg ttcgggggcc ttggtcttgt catgcgcgag cgtcacgcgc
    10021 gcgttaatgg caaaccggtc aagacgcgcc aatttgtcca cccgattgac aatgtgcgcc
    10081 tcgatggccc gggtcaaagt caggttatgc gtcgcaagga tcaagttcat gccccctaca
    10141 atgccgcccc ccggaaataa aatccagcga aactattttc cgccccgctc cccggggaaa
    10201 cacccccgcc gcgcgcgcgg gcttgaatcc acgcccgctt tccgctaaac cgttcgcgtg
    10261 ccaaccaaag ttatcgccgt cgccaaccag aaaggcggcg tcggcaagac gaccaccgcg
    10321 gtcaacctgg ccgcctgtgt cgccgcggtc ggccggcgcg tgctcctctt cgacctggat
    10381 ccgcaggcca acgcaaccag cggagtgggg ctggagaaaa cgcccggcgc cagcgcctat
    10441 ctcgcgctgc tggggcaggg ccgcttgcag gacaaggttc gcaacacggc gttcgcccgc
    10501 ctggcggtca tccccgggga ggtggatctg tgcggggcgg acctcgaact ggcgcgcgcg
    10561 gacaaccacc tgcagcgcgt tcggctggcg ctgcaacccc tccgcgagga gcggcgcttc
    10621 gacgtgatct tcgtggattg cccgccttcc ctgggcgtgc tcacgctcaa tgccctggcc
    10681 gccgccgacg gcgtgctcgt gccgctccaa tgcgaatact acgccctcga gggcctctcc
    10741 atgctcaatc gcatcatgac ccagttgcac gacacgggcg tcaatccccg gctggagctg
    10801 acgggagtgg tgatgaccat gtacgacagc cgcaccaaac tttcccaaca ggtcgtcggg
    10861 gaagtgcgcc agcatttcgg cgagcgggtc tttgagaccc tgatcccgcg cagcacgcgc
    10921 ctggcggagg ccccgagcta cggccagccc attattcact acgacaaata cagcgccggc
    10981 tccgcggctt atgaattgct ggcccaggaa ttcctggcgc ggctggcgca agaccgcgaa
    11041 ccccggcaac ccggcccgcc gcgttccgaa aaccaataaa caactccacc cggccgggag
    11101 caaacctgac aatatgagca acaaagttaa atggggagtg atcggttgcg gcggcattgc
    11161 ggcccggcgc accatccccg aactcaaaaa actggccgct aacgcggagc tggtctccgt
    11221 gatggacacc aacgcggcgc gcgcccggga agtcgccgcc caattcggcg tgccgcacgc
    11281 ctgcgccacc gaggcggaac tgctcgccca ggacgtgcag gccgtgtata tcgcctcgcc
    11341 ccccaacgcc cattgccgcc aaaccgtgca ggccgcccgg gcgggaaagc atatcctgtg
    11401 cgaaaaaccc atcgccgtct ccgtggcgga ggtggaccag atggaagccg cctgcgcgca
    11461 agccggcgtg aagttcatgc tcgcctggtg catgcggcaa aacgtctatc accagcaggc
    11521 gcgcgaactg gtgcaatccg gcgcgctggg ccgaatggtg atgggccgcg cgcagttgac
    11581 ctgctggtat ccgcccattc ccggcgcctg gcggcaggac atcgccctca gccacggtgg
    11641 cgcgttgctg gacatgggca cccattgcct cgacctcctg gaatggattt tcggctcgcc
    11701 catcatcgag gtgaccggct ttaccgacct gctcatccac gcatacgcca cgcccatcga
    11761 agacaccgcg accatcctgg tccgcttccg caatggcgcc cacggtatca ttgataacta
    11821 tttcaatatc ccggacgccg ccgcgcaaaa cgcgctggaa ctcttcggca ccaagggctc
    11881 gatcattgcc cgcggcacca tcggccagga gccgaccggc cagatgttca gcatcctgca
    11941 ggcccaggaa accggctacg acgccgccca aacgcgcgat tcccagccca accgccggga
    12001 atacaagctg gaaggccgcg gcatctacgg gcagatgatc acgctgttct ccgactgcat
    12061 cctgcaagac cgtccgcccc cggcttcgct ggccgacggc cgccattcgg tcaaggtcgt
    12121 caatgccatc taccaggccg cccgcgaacg gcgcgtagtc gaggtcgccg cctgacaacg
    12181 gcatccggcc ttgacccgca aaagcgggcc aaggcagcca gccaac
//
LOCUS       DAOPQG010000103        12191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6037_length_12191_cov_15.201879, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000103 DAOPQG010000000
VERSION     DAOPQG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6037_length_12191_cov_15.201879"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..860
                     /locus_tag="PKI07_10995"
     CDS             105..860
                     /locus_tag="PKI07_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC51616.1"
                     /translation="MTGSESQKTCLHERLLAVPPDQIEERLAQLDRLATLGTLAAAMA
                     HEIRNALVPGKTLVGLLLEKQPEDELAGMVRREIGRIDAIVTRMLKFSGSAQLNLSEL
                     RLHEVLEHSLRLIQAQLEGKYISLHRSFQAAADRVRGDEYQLQQAFVNLLMNALEATG
                     YNGTIAVATETLAASDGAPARLQVTIRDNGSGIAPEVMSRLFEPFFTTKPQGTGLGLP
                     ITRRIIREHHGDLSVESQPGQGATYHIFLPVLS"
     gene            943..1950
                     /locus_tag="PKI07_11000"
     CDS             943..1950
                     /locus_tag="PKI07_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51617.1"
                     /translation="MRTCTLGILSDIHYAGAAEQARGRDYELRAIARPLVRAGATAFR
                     RFVWRRNPFAGNARLEQFLQRAGGFDYVIANGDYSCDSAFVGLSDEAACQSARECLGK
                     LRQRFGGRLRVNYGDHELGKLSLFSAHGGLRLASWHRARTDLGLSPLWRLDLDRYVLL
                     GFVSSLVALPALAGDMLPAERPEWERLRQEHLAEITAVFAALRPDQRMLLFCHDPTAL
                     PFLGREEVIRARLPQIELTVIGHLHSNLVLWQSRLLAGMPPLGGLGRAVRRFSTALNQ
                     ARQWRPFRVRLCPALAGIELLKDGGYCTIQLDPEARQPLRFQFHRLRGAGAGGGDYKK
                     G"
     gene            complement(1935..4127)
                     /gene="lptD"
                     /locus_tag="PKI07_11005"
     CDS             complement(1935..4127)
                     /gene="lptD"
                     /locus_tag="PKI07_11005"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOC51618.1"
                     /translation="MTRLRLMFLLALGALALASAARAQQQPAWEMEALTDEGYAEFDF
                     QTGLGRGTNGVLVRYGNAFLTADQVTVNQQTGEVAAEGQVRLQNEDQLWIGEHIRYNF
                     KTRQLNAEQFRTGKAPVFAAGEGLHLELTNRLYRATNGVITTDDVADPAIKVRARHLK
                     IIPGDKVVASGATLYVAKVPVFYFPYYSRRLSPRANHFTFIPGYRSSFGPFLLSGYNF
                     FLNDELDGVAHVDYRVRRGLGLGPDVNYHLGRWGDGTFKYYYLYDQDPQARDSQNVSP
                     HNRQRVYFSYQANPATNVYAQAVVRYQGDTNIIREFFENEYRRNPQPSTFVELNKFWR
                     NFSLDAYVQPRVNDFLETVERLPDVRLTGYRQQLGNSPLYYESESSAGYYRRLFVETN
                     SLVSPPDFAAARADTYHQLLLPQTLFGWLTVTPRAGGRLTYYSRATERGATTDERWRG
                     VFNTGAELSFKASHLWPDAQSGFFEVNGLRHILQPSANYAYVPQPNYRGPRELPQFDY
                     ELASLRPLPIEFPDYNAIDAIDSQHVLRLGLRNKLQTKRNDQVVNLVNWEVFTDWRLQ
                     RQARQGAFADLYSDLTLRPRSWLHLDSELRYSVENGYLRLSQTRLGLQPGARWSWAFG
                     HLYLRDDLSPEPTAYGRGNNLFSSSLQYRLNENWGFRAVHWFEARSGTLQEQAYSVYR
                     DMRSWTGALTLKVRNSQGRPQDVTVAFTFWLKAFPGSGRDPRADQPFL"
     gene            4257..4475
                     /locus_tag="PKI07_11010"
     CDS             4257..4475
                     /locus_tag="PKI07_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51619.1"
                     /translation="MAAKVNMMKLLQEIRGFAAITNRRPPPRFPGLLLMQMRDRRAQD
                     KLSQRRLNITIDTQRGVEAPNPVRIHHR"
     gene            4873..5265
                     /locus_tag="PKI07_11015"
     CDS             4873..5265
                     /locus_tag="PKI07_11015"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOC51620.1"
                     /translation="MNTALFAQLVMQQSSMAMMLLGKTPHPASGQPVRDLEGAQLFID
                     QLEMLEVKTKGNLIPEETAILKQSLMSLRLAFVECVDAPPAPAPDPASAATPPGEAAA
                     AAKPDAPAAAPSSVSGEEESRKKFTKKY"
     gene            5851..7848
                     /locus_tag="PKI07_11020"
     CDS             5851..7848
                     /locus_tag="PKI07_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51621.1"
                     /translation="MNATKWYGMVTGMLIPVFAGFAQAPAATEAPAPGATEVAVPANL
                     SPGAAEVIRLATSGVGDEVVLAYIRNSQAQFNLSADNVLYLKDLGLSEEVTSAMLNHD
                     GTLRGQEQSYAPTAANVAPVAATPAAPAAPAAAPATAPAAAPAAAPAAVAAPAPVYVS
                     SPPPDVTYFYDSLAPYGTWVQLDGYGWCWQPTAVVVTPGWRPYCNGGYWVYSDYGWYW
                     QSTYSWGWAPFHYGRWHLHARCGWVWLPDRVWGPAWVTWRTGGDYCGWAPLPPYAVFD
                     VHHGWRYNGVSVSAGFGFGLGSDAFLFVSFNNFFHRDLRRHCLPRSRVGPVYNQTTIV
                     NNYIVNNNRIENRGIPVDRFSSAHRGPIPRATVREWNDRPDRMPTRAGSVVYRPQLNA
                     PARTSSMVAQRVDAQHPTIRHTPSAPPARGQAFAGGNRTRPEPSRPITGTSGTPPSSA
                     SRFQSTPASATPAATPPANRGRTTSDWTSRPRASSAPSTPSTSGAAPATPSGPASRTG
                     LTSSSARPSAPTPAQPSTGTSRTTPSASGTRSTTTSTWTSSARPTPAPVAQAAPSAST
                     TVPRIPAGPPAPRAPEEWKQGVRPSPGYRSDAGLPSLYNTRPAAPASAGNSRPATPHF
                     DQPKTQQQAAGVRSLPSTRNYSPAGSASRPFRSPSGSRSGR"
     gene            8069..8968
                     /locus_tag="PKI07_11025"
     CDS             8069..8968
                     /locus_tag="PKI07_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC51622.1"
                     /translation="MKVLIVGSTALDSIKTPKAENPRLLGGSASHAAVAASFFGPVQL
                     VGVVGQDFPGRYLRLYQRHGIDLAGLQRRPGRTFHWSGEYAANMNQRRTLRTELGVFE
                     QFTPELPPAYRDTPFVLLANIAPGLQHHVLNQMRRPRFVAADTMDLWLKVARSELLRL
                     LRRLDLFVLNDSEAQQLTGEENPLAALPLIHRRGPKYVVIKQGSHGSILSGPHGRFIC
                     PAYPLRRVVDPTGAGDSFIGGLMGCLAAARGPVERHLRRAMVFGSVVASFCCEGFGVQ
                     RTTRVTRAQIDARVRELEQLTRF"
     gene            complement(8969..9889)
                     /locus_tag="PKI07_11030"
     CDS             complement(8969..9889)
                     /locus_tag="PKI07_11030"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOC51623.1"
                     /translation="MQLNVTQLADRLMASYASAGGINHWDGKNLPSKRAIVLITADLL
                     RLLFPGYFDEKLVHSSELRATTAALLESVLRSLENEIAKSLEYHPPPDLPGKGLRPFA
                     RALTREFLASLPRLRELLQTDTEAAYNGDPAALSREEVIVAYPFIEAIAVQRLAHELY
                     AKNIPLIPRIMTEWAHARTGMDLHPGAQIGSHFFVDHCTGTVVGETTRIGSHVKMYQG
                     VGLVAKSLALGQQLRGQKRHPTIEDRVTIYAGATIMGGDTVVGEGSTIGANVFLTTSV
                     PPHSLVIQEEANVRVLKKKERSRPPDGAAL"
     gene            complement(9968..11134)
                     /gene="waaF"
                     /locus_tag="PKI07_11035"
     CDS             complement(9968..11134)
                     /gene="waaF"
                     /locus_tag="PKI07_11035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOC51624.1"
                     /translation="MTAPPHSFAGLPDRILIRGVNWLGDAVMTTPALQRLREARPRAR
                     LVLLTQEKLAGLWQNHPSLDGLLTCAAGESPWAVAGRLRAEAFSTALLLPNSPRAALE
                     AWLARIPQRIGYARPWRNWLLTQPLPPRPGQARMAKRSVREIRRLIRRSGASTAGAPV
                     SPPPRASHQIHDYLHLAVALGANPEPLAPRLEVTADELRQTEEAFLPGKRGQAQASPL
                     AVSRRLLGLCPGAEYGLAKRWPAERFAAVARAVVREDANCLWLALGGPRDWPVCEEVA
                     RRAGGGVLNLAGQTSLRQLMALLKLCRVVLTNDSGPMHVAAALGTPVVVPFGSTSPAL
                     TGPGQPGDPRHRLFSSDAPCAPCFRRHCPIDLRCLTAISADDVTQAVRQILAAL"
     gene            complement(11147..12100)
                     /locus_tag="PKI07_11040"
     CDS             complement(11147..12100)
                     /locus_tag="PKI07_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51625.1"
                     /translation="MDTLLYLLARALVAGLQALPLTVVARLGRFGGGLAYGLDARHRR
                     VALANLARCFGAEQSPARLRALAREHFRRLGENYACAIKTASMGLAELRPHVEFAGNL
                     RLFAVRAGERPASVVCAIGHFGNFELYARFGQFAPAYQCATTYRGLRPPALNRLLQSL
                     RERSGCRYFERRFDADALKAFMRQRGVVLGLLADQSGGALRGPFLGHECLTSAAPAIF
                     ALRYRCPLITAICYRVGLARWRIELGEEIPTRENGRPRSTAAIMADVNRAFEVAIRRD
                     PANWFWVHRRWKPPRPGRRRAIPNAPPSAEAAAVASSGSAG"
BASE COUNT         2068 a         3971 c         4145 g         2007 t
ORIGIN      
        1 ggcaaacggc ctggttggtt caaagaaagc cgggcggttt ccgcttgccg cgcctccggg
       61 gggtgagtta gcctgtgaac tgcgcatggc catgcccaac cgttatgacc ggtagtgaga
      121 gccaaaagac ctgtttgcac gagcggctgc tcgcggttcc gccggaccaa atcgaggaac
      181 ggctggcgca actggaccgc ctggccacgc tcggcacgct ggccgccgcg atggcgcacg
      241 agattcgcaa cgcgctggtg ccgggcaaga cgctggtggg gctgctgctg gagaagcaac
      301 cggaggacga gctggcgggc atggtgcgtc gcgaaatcgg ccgcattgac gcgatcgtca
      361 cccgcatgct gaagttctcg ggttcggcgc agttaaacct gagcgagctt cgcctgcacg
      421 aggtcctgga gcattcgctg cggctcatcc aggcgcaact ggagggcaag tatatttccc
      481 tgcaccggtc gttccaggcc gccgctgacc gggtgcgggg ggatgaatac cagcttcagc
      541 aagcctttgt gaacctgctg atgaatgcgc tcgaagcgac cggctacaac ggcacgatcg
      601 cggtggccac ggaaaccctc gccgccagcg acggcgcccc ggcgcggctc caggtgacga
      661 ttcgggataa tggctccggg attgcgccgg aggtcatgag ccggctgttc gagccgttct
      721 ttaccaccaa gccccagggc acggggcttg gcctgcccat tacccggcgc attatccggg
      781 agcaccacgg cgacctcagc gtggaaagcc agcccggcca gggcgccacc taccatatct
      841 ttctgccggt tttatcctga cgcgcccgtc cgccgccgcc gccggcccgc gggcgggtcc
      901 gccgggttga cggcgcccgc cggactgcgt caggatggcc ccatgcgcac ctgcacgctc
      961 ggcatcctga gcgatattca ttacgccggc gccgccgaac aggcgcgcgg gcgcgattac
     1021 gagctgcgcg ccatcgcccg gccgctggtc cgggccggcg cgacggcgtt ccggcgcttt
     1081 gtctggcggc gaaatccctt tgcggggaac gcccggctcg agcagtttct ccagcgcgcc
     1141 ggcggctttg attacgtgat cgccaacggg gattattcct gcgactcggc gtttgtgggc
     1201 ttgagcgatg aagccgcctg ccagagcgcg cgggaatgcc tcggcaaact ccggcagcgg
     1261 tttggcgggc ggctgcgggt aaattacggc gatcatgagc tgggcaaact gtccctgttc
     1321 agcgcgcatg gcggcctgcg gctggccagt tggcaccggg cgcgcaccga cctggggctg
     1381 tcgccgttgt ggcgcctcga cctggaccgt tacgtgctgc tcgggtttgt gtcctcgctg
     1441 gtcgcgctgc cggcgctggc cggggacatg ctgcccgccg agcggcccga gtgggagcgg
     1501 ctgcggcagg aacacctggc ggaaatcacc gccgtctttg cggccctgcg gcccgaccag
     1561 cggatgctgc tgttttgcca tgacccgacc gccctgccgt ttctcgggcg cgaggaggtc
     1621 attcgggcca ggctgccgca aatcgagctg acggtgatcg gccatctgca ttccaacctc
     1681 gtgctgtggc agagccggct gctggcggga atgccgcccc tgggcggcct gggccgcgcg
     1741 gtccggcgct tcagcaccgc gctgaaccag gcccgtcagt ggcgcccctt tcgcgtgcgt
     1801 ctttgcccgg cgctggcggg catcgagctg ctgaaggatg gcgggtattg cacgattcaa
     1861 ctggatcccg aagcgcggca gcccctccgc tttcagtttc accgtctgcg gggcgccggg
     1921 gcgggcggcg gggattacaa gaagggctga tccgcccggg gatcgcggcc ggagcccgga
     1981 aacgccttga gccagaaggt gaacgccacg gtgacgtcct gggggcggcc ctggctgttg
     2041 cgcactttga gcgtgagggc gcccgtccaa ctgcgcatgt cgcgatagac ggaatatgcc
     2101 tgctcctgca aggttcccga ccgggcctcg aaccaatgca ccgcccggaa cccccagttc
     2161 tcgttcagcc gatactggag cgagctgctg aaaaggttgt tgccccgtcc gtaggcggtg
     2221 ggctcggggc tcaggtcatc ccgcaaataa agatggccga aggcccaact ccagcgggcg
     2281 ccgggttgga ggccaaggcg ggtttgcgac agccgcaaat agccgttctc cacactatac
     2341 cgcagctccg aatcgagatg cagccatgag cgcgggcgca gcgtgaggtc ggaatacagg
     2401 tcggcaaacg cgccctgcct ggcctggcgc tgcaggcgcc agtcggtaaa gacctcccag
     2461 ttgaccaggt tgaccacctg gtcgttgcgt ttggtctgga gtttattgcg caaccccagg
     2521 cgcagcacat gctggctgtc tatggcatct atggcgttgt agtcgggaaa ctcaatcggg
     2581 agcgggcgca ggctggccag ctcgtagtcg aattgcggca gctcgcgcgg gccgcgataa
     2641 ttgggctgcg ggacgtaggc gtaattggcc gagggttgca ggatatgccg caggccgttc
     2701 acctcgaaaa agccgctctg cgcgtccggc caaagatggg aggctttgaa ggaaagctcg
     2761 gcgccggtgt tgaacacgcc gcgccagcgc tcgtcggtcg tcgcgcctcg ctcggtggcc
     2821 cggctgtaat aggtgagccg cccgcccgcg cggggggtga ccgtgagcca gccaaagagc
     2881 gtctgcggga gcagcagttg atggtaggta tctgcgcggg ccgcggcgaa gtcgggaggg
     2941 gaaaccaggg aattggtctc cacgaaaagc cggcggtagt agcccgcgga actctcgctt
     3001 tcatagtaaa gcggcgagtt gcccaattgc tggcggtagc ccgtcagccg cacgtcgggc
     3061 aggcgctcga cggtttcgag aaagtcgttg acccggggct ggacataggc atcgaggctg
     3121 aagttgcgcc agaacttgtt gagctcgacg aatgtgctgg gctgcgggtt ccggcggtac
     3181 tcgttttcaa agaactcacg gatgatgttg gtatcgccct ggtagcgcac gaccgcctgg
     3241 gcgtacacat tggtggcggg gttggcctgg taggagaaat aaacccgctg ccggttgtgc
     3301 gggctgacgt tctgcgaatc gcgggcctgc gggtcttgat catacaggta gtaatacttg
     3361 aacgtcccgt cgccccagcg gcccaggtgg tagttcacgt ccggccccag ccccagcccc
     3421 cgccgcaccc ggtaatccac gtgcgcgacg ccgtccagtt cgtcgttcag gaaaaagtta
     3481 tagccgctga gcagaaacgg cccgaagctg ctgcgatacc cggggatgaa cgtaaaatga
     3541 ttggcgcggg gggacagccg ccgcgagtaa taggggaaat agaaaaccgg caccttcgcc
     3601 acgtagagcg tcgccccgct cgccaccacc ttatcccccg ggatgatctt gaggtgtcgg
     3661 gcgcgaacct tgatcgccgg atcggccaca tcatcggtgg tgatgacgcc gttggtggcc
     3721 cggtagaggc ggttggtcaa ctccaggtga agcccctccc cggcggcaaa caccggcgcc
     3781 ttgccggtgc gaaactgctc cgcgttcaac tgccgcgttt tgaaattgta gcggatatgc
     3841 tcgccgatcc acaactggtc ttcgttctgg agccgcacct ggccctcggc cgcaacctcg
     3901 ccggtctgct gattcacggt gacctggtca gcggtcagga acgcgttgcc gtagcggacc
     3961 agcacgccgt tggtgccccg gcccaggccc gtctggaaat caaactcagc gtagccctcg
     4021 tcggtcaacg cctccatttc ccaggcgggt tgctgctgcg cccgggccgc ggaggccaag
     4081 gccaaagcgc ctagcgccag caggaacatg agccgcagtc gtgtcatttg gtgccggaag
     4141 ctaagcagaa gccgcccgcc ctgccaagcg gcaattccgc cgccccgcgc gccgtccaag
     4201 cccgggcggc cccggactgg cacgtgaact gcagacgctc gccctggaat tgtcctatgg
     4261 ccgccaaagt caacatgatg aaattgctcc aggagatccg gggctttgcc gcgattacca
     4321 atcggcgccc gcccccgcga ttccccgggc tgctgctcat gcaaatgcgc gatcggcgcg
     4381 cccaggacaa actgagccag cgcaggctga acatcacgat tgacactcag cgtggcgtcg
     4441 aagcgccaaa cccggtcaga atccatcatc ggtagccaaa tcccggcttt ggcgcgggaa
     4501 tccacgcccg aaaaaacact tgccctcctc acccttgcct cgccctttcc tccgggaaac
     4561 ctccgctatt gtaatagcga gagaatgcca gagatttgcg tggatgaaac aaagccggac
     4621 ggaatagggg gctcccggcc cgcccgccaa gcaaagctgc gcgacggagg aaggaatcgc
     4681 cgtgctccgg ccggcgggtt ggctgaatgt tcagcttttg ccagccgtgc cttaaaatga
     4741 ggcagtgccg acgggggctt gcgggcggcg acattttttg ctggccgaaa ttcgcgctgg
     4801 ccgcaaagct ggttccgcat gaatgattcg cacctttctg aaaaagcgcc cgcagcgaac
     4861 cgccctgaag atgtgaacac cgcactcttt gcgcaactgg tcatgcaaca gtccagcatg
     4921 gccatgatgc tgttgggaaa aactccgcac cccgcctccg gccagccggt tcgcgacctc
     4981 gaaggggccc agctcttcat tgaccagttg gaaatgctgg aggtgaagac caaaggcaac
     5041 ctgatcccgg aagaaaccgc gatcttgaaa cagagcctga tgtcgttgcg cctggctttt
     5101 gtggaatgtg tggatgcccc gcccgcgccg gctcccgacc cggcaagcgc cgccacgcca
     5161 cccggcgaag ccgccgccgc cgccaaaccg gacgcccccg ccgccgcacc gtcctccgtt
     5221 tcgggcgaag aggaatcccg caaaaaattc accaagaagt attgagcaaa acacaggctc
     5281 cgcgggaatg gaggcgcggg gcgtgatgcg gaagcgcgtg atttggattg gccggggaag
     5341 gcgggcccgg caaaaagctg gattcacgca ggcgattgtt cgtgggttca gcccgggtat
     5401 ttcaggcgcc gggcgggctg cccgcagtcc ggcgcacgag ctccggtctc gcatttgaca
     5461 ttaagcgggc atgagcgatg ttttcagtgg ggtagttgtg ggatttgcta gtaggttgtt
     5521 tgggcgtagc ctggcgaggc tgcgccttta cttcaggcac gggcgatgtg gattggagga
     5581 cggcgggttt gcgttccttg attcaaccac ggactgtggc ggcggcgggc ttcatttgcc
     5641 gcgggtgaag ccgggaagcc cggcgcgctg gctggttggc gcccgagggg aactggggcg
     5701 ggcatctggc cgagcgcgcg gggcgaagtt tcgactggcg gcggcggcgg gggtcttcta
     5761 tcttgcgtca tggcagctaa atgcggcggt gtgaataaag cgtgggctga taggtctaac
     5821 ccgatagtga agataacgta gtcgagtact atgaatgcaa caaaatggta tggaatggtg
     5881 accggaatgc taattccggt attcgctggt ttcgcgcagg ctcccgcagc cactgaggcg
     5941 cccgcgccgg gcgcgactga agttgcggtc ccggccaatc tctcgcccgg cgccgccgaa
     6001 gtcatccggc tggctacctc gggggttggg gatgaagtgg ttttggctta catccggaat
     6061 tcgcaggccc aattcaatct ttccgccgac aacgtgcttt acctcaaaga ccttggactt
     6121 tcggaagagg tcacttcggc catgttgaac catgacggca ccctgcgagg ccaggagcag
     6181 tcgtatgcgc ccaccgccgc caatgtggcg ccggtcgcgg cgacacccgc tgccccggcc
     6241 gctccggcgg ccgcgcccgc tactgcgccg gccgccgccc ctgcagcggc acccgcggcc
     6301 gttgcggctc cagcgccggt ttatgtcagc agcccgccac cggacgtgac atatttctat
     6361 gacagccttg cgccgtacgg cacatgggtg cagttggatg gctatggctg gtgctggcag
     6421 cccacggcgg tggtggtgac gccgggctgg cggccgtact gcaatggcgg ctactgggtt
     6481 tacagtgatt acggctggta ttggcaatcc acgtattcat ggggctgggc accgtttcat
     6541 tatggacgct ggcatctgca cgcgcgctgc ggctgggtct ggctgccgga ccgcgtttgg
     6601 gggccggcct gggtgacctg gcggaccggt ggcgattact gcggctgggc gccgttgccg
     6661 ccctacgcgg tgtttgatgt tcatcatggc tggcgctaca acggcgtcag cgtcagcgcc
     6721 ggctttggtt tcggcctggg cagcgacgct ttcctgttcg tcagctttaa caacttcttc
     6781 caccgcgacc ttcgtcggca ctgtctgccg cggtcccggg tgggcccggt ctataaccag
     6841 acgaccattg tcaacaacta catcgtgaac aacaaccgga tcgaaaaccg ggggattccg
     6901 gttgatcggt tttcctccgc ccatcgcggc cccattccgc gggccacggt gcgcgaatgg
     6961 aatgaccgcc cggatcgtat gcccacccgc gccggctcgg tggtttatcg gccgcaactg
     7021 aacgcgcccg cgcggaccag tagcatggtc gcccagcgcg tggacgcgca acacccgacc
     7081 atccggcaca cccccagtgc gccgcctgcg cgcggccagg ccttcgccgg gggaaaccgc
     7141 acgcgcccgg agccctcccg gccgatcacc ggcacgagcg gcaccccgcc ttcctcggcg
     7201 tcgcgcttcc aatctacgcc ggcttccgcc acgccggcag caacgccgcc ggccaatcgc
     7261 gggcggacca cctcggactg gaccagtcgc ccgcgcgcct cgtccgcccc gtcgacgccg
     7321 tcaacgtccg gagccgcccc cgcaacgccg tccggaccgg cttcccgaac cgggctgacg
     7381 tccagcagcg cccggccgtc cgctccaaca ccagcccagc cgtcaaccgg caccagccga
     7441 accacgccgt cggcctcggg aacgcggagc accaccactt cgacctggac cagcagcgcg
     7501 cgcccaacac ccgcgcccgt cgcgcaggcc gcgccaagcg cttccacaac cgtaccgcgc
     7561 atcccggccg ggccgcccgc cccgcgcgcg cccgaggagt ggaagcaagg agttcgtccc
     7621 tcgcccggct accgatcgga tgcaggattg ccgtctttgt ataacacgcg cccggccgcg
     7681 cccgcgtccg ccggcaactc ccggccggcg acgccgcact tcgatcagcc caagacgcag
     7741 caacaggcgg cgggtgtgcg ctcgctgcct tccaccagaa actattcgcc cgccggatcc
     7801 gcttcccgcc ccttccggtc gccctccggc tcccggtcgg gccggtaacg tttggcgctt
     7861 cggcgcatcg gcggcgcggc tgcgccgccg cagattgccc gcgcacggaa agcggcggcg
     7921 ggccgccgtt gcccgcgggc acatttttac tcaccttccc gcgcggctta tgttataaaa
     7981 agaggcatga gaccgttttc gccagcaaag cccccccgcc ggggaagccc ggggcggggc
     8041 cgcggacaca accctgaata acctcgcgat gaaagttctt attgtcggat cgaccgccct
     8101 ggattccatc aaaaccccca aagcggagaa cccgcgcctg ctgggcggct cggccagcca
     8161 tgcggcggtg gcggcgagtt tctttggccc ggtccaattg gtcggggtgg tggggcagga
     8221 tttccccggg cgttacctgc ggctttatca gcgccacggg attgacctgg cgggcttgca
     8281 gcggcggccg ggccggacgt tccattggtc gggcgagtat gccgcgaaca tgaaccagcg
     8341 acgaacactg cgaacggaac tgggggtgtt cgagcaattc acgcccgaat tgccccccgc
     8401 gtaccgggac acgccctttg tgctgctggc caacatcgcg ccgggcctgc agcatcacgt
     8461 gttgaaccaa atgcggcggc cgcggttcgt ggcggcggac accatggact tgtggctcaa
     8521 ggtggcgcgc tcggagttgc tgcggctgct ccggcggctg gatctgttcg tgctcaacga
     8581 cagtgaggcg caacagttga ccggggagga aaacccgctg gccgcgctgc cgctgattca
     8641 ccggcggggg ccaaaatacg tggtcatcaa gcagggatcg cacggctcga tcctgtccgg
     8701 gccgcacggg cggttcatct gcccggctta tccgctgcgc cgggtggtgg atccgacagg
     8761 cgccggggat tcgttcatcg gtgggctgat gggctgcctg gccgcggcgc gggggccggt
     8821 cgagcgccat ctgcggcggg cgatggtctt cgggagcgtg gtggcgtcgt tctgctgcga
     8881 agggttcggc gtgcagcgca cgacccgggt cacccgggcg cagattgacg cgcgggtgcg
     8941 ggaactggag cagttgacgc gcttttaatc agagggccgc tccgtcgggc gggcgggagc
     9001 gttccttctt cttcaggacc ctgacgttgg cctcttcctg gatgaccagg gaatggggcg
     9061 gcacgctagt ggtgaggaag acattagccc cgatggtgct gccttcaccc acgaccgtat
     9121 ccccgcccat gatggtcgcg ccggcgtaaa tggtcacgcg gtcttcgatg gtggggtggc
     9181 gtttctgccc gcgcaattgc tgccccagcg ccagcgattt ggccaccaac cccacgcctt
     9241 gatacatttt cacatggctg ccgatgcggg tcgtttcccc caccacggtg ccggtgcaat
     9301 gatccacaaa gaaatgggag ccgatttggg cgcccggatg taaatccatg ccggtgcgcg
     9361 cgtgggccca ttcggtcatg atgcgcggga tgagggggat attcttcgca tacaactcat
     9421 gcgccaggcg ctgcactgcg atggcctcaa taaaaggata ggccacgatc acttcctcgc
     9481 ggctgagggc tgcggggtcg ccattgtagg cggcctcggt gtccgtttgc agcagttcgc
     9541 ggaggcgggg caggctggcg aggaactcac gggtcagggc gcgggcaaac gggcgcaggc
     9601 ccttcccggg taagtcgggg ggcggatggt attcgaggct cttggcaatt tcattttcca
     9661 ggctgcgcag cactgattcc agcagcgcgg ccgtggtcgc ccgcaattcc gaggaatgca
     9721 ccagcttctc gtcgaagtag ccgggaaaga gcaaccggag caggtcggcg gtgatcaaca
     9781 caatcgcgcg cttggaaggc aggttcttgc cgtcccaatg gttaatgccg ccggcgctgg
     9841 cataagaggc catcagacga tcggcgagct gggtcacgtt taactgcata ccttgagtat
     9901 ctggcaaagc ctggccgggc gcaaggcgtg cccgcagacg ccggcccggc cccgaaccgc
     9961 ccgccgccta cagcgcggcg aggatttgcc gcaccgcctg cgtgacgtcg tcggcggaaa
    10021 tagcggtcag gcagcgcaaa tcaatcgggc agtggcggcg gaagcacggc gcgcacggcg
    10081 cgtccgagga aaacagccgg tggcgcggat cgccgggctg gccggggccg gtcaatgcgg
    10141 gcgacgtgct gccaaacggg acgaccaccg gggtgcccag cgcggcggcg acgtgcatcg
    10201 ggccgctgtc attcgtcagc accacgcgac agagcttgag cagagccatg agctgccgca
    10261 gggaagtctg cccggccagg ttcagcaccc cgccgccggc gcgccgggcc acttcctcgc
    10321 acaccggcca gtcgcgcggg ccgccgagcg ccagccaaag acagttggca tcctcgcgca
    10381 cgaccgcgcg cgcgacggcg gcgaagcgtt ccgcgggcca gcgtttggcg aggccatatt
    10441 cggcgccggg acacaatccc agcagccgcc gggacaccgc caagggagaa gcctgcgcct
    10501 ggccgcgctt ccccggcagg aaggcctcct ccgtctggcg taattcgtcg gcggtgacct
    10561 ccagccgggg tgcgagcggc tcgggattgg ctcccagcgc caccgccagg tggagataat
    10621 catgaatttg atgggaggcg cgcggcggcg gggaaacggg ggcgcccgcc gtggaggcgc
    10681 cggaccggcg aatcagccgt cttatttccc ggaccgaccg cttcgccatg cgcgcctggc
    10741 cggggcgcgg gggcagcggt tgcgtcagca gccagttccg ccagggccgc gcgtacccga
    10801 tgcgctgcgg gatgcgcgcc agccacgctt cgagggccgc gcgcggcgaa ttgggcagga
    10861 gcagggcggt ggaaaaagcc tccgcgcgca accggccggc caccgcccac gggctttcgc
    10921 cggcggcgca ggtgagcagg ccatcgaggc ttggatggtt ttgccacaag ccggcaagct
    10981 tctcttgcgt gagcaaaacg aggcgcgccc ggggccgcgc ttcccgaagg cgctgcagcg
    11041 cgggcgtggt catcaccgcg tcgcccagcc aattcacacc gcggatgaga atgcggtccg
    11101 gaaggccggc gaaggaatga ggcggcgccg tcatgcgtaa tccaggtcaa cccgccgagc
    11161 cggaggaggc gaccgccgcc gcttccgcgg acggcggggc attggggatg gcgcggcgcc
    11221 ggccggggcg cggcggtttc caacggcgat gcacccagaa ccagttggcc ggatcgcggc
    11281 gaatcgccac ctcgaaggcg cggttcacgt cggccatgat cgcggcggtg gaacgcggcc
    11341 ggccgttctc gcgggtgggg atttcctccc ccaactcgat gcgccagcgg gccaggccga
    11401 cgcggtagca aatggcggtg atcagaggac agcggtagcg cagcgcaaag atggccggcg
    11461 cagccgaggt gaggcactca tgccccagga acggcccgcg cagcgcgccg ccggactggt
    11521 cggccagaag ccccagcacg acgccccgct gccgcatgaa agccttgagc gcgtcggcgt
    11581 caaaccggcg ctcgaaatag cggcagcccg accgctcgcg cagggattgg agcaggcggt
    11641 tgagcgcggg ggggcgaagc ccgcggtaag tagtggcgca ttggtaggcg ggcgcaaact
    11701 gcccgaaccg ggcgtacaac tcgaagttgc cgaagtggcc gatggcgcac acgacgctgg
    11761 cgggccgctc gccggcgcgc accgcaaaaa ggcggagatt gcccgcaaac tccacatgcg
    11821 ggcgcagctc ggccaggccc atggacgcgg tcttgatcgc gcaggcataa ttctccccca
    11881 ggcggcggaa gtgctcgcgc gccagcgcac gaagacgggc cggcgattgc tccgccccga
    11941 aacaccgcgc gaggttggcc agcgcgacgc gccggtgccg cgcatccagc ccgtaggcca
    12001 gcccgccgcc gaatcgccca agccgcgcca ccaccgtcaa cggcagcgcc tgcaaccccg
    12061 ccaccagggc gcgcgccagc agatatagca gggtatccat gcctagcgga aacagatttt
    12121 gcgcacgcaa tcctggaagt catccgcccc gcggagaatc ttgatctcga cgcgcataaa
    12181 gcggatcggc a
//
LOCUS       DAOPQG010000104        12159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6067_length_12159_cov_27.425479, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000104 DAOPQG010000000
VERSION     DAOPQG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12159
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6067_length_12159_cov_27.425479"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            469..867
                     /gene="crcB"
                     /locus_tag="PKI07_11045"
     CDS             469..867
                     /gene="crcB"
                     /locus_tag="PKI07_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOC51626.1"
                     /translation="MFAYFWVATGGALGSVARYWLSGVVAQQTGATFPWGTILVNASG
                     SLVIGVFAALTGADGRWLVPDWFRQQFFMLGLCGGYTTFSSFSLQTLNLAQAGEWLYA
                     SANVVLSVLTCLAAVWLGHGLAAALNVVSR"
     gene            934..1311
                     /locus_tag="PKI07_11050"
     CDS             934..1311
                     /locus_tag="PKI07_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOC51627.1"
                     /translation="MPLPREAVLLRIFIGESDRWGHQPLYEAIVLKARELHLAGATVL
                     RGPMGFGQSSRLHTAKILRLSADLPLVIEIVDRPEKIQSFLPVLEPMLAGGLLTLEKV
                     TVIEPRAGRAAESARPEPGPFEA"
     gene            1448..3283
                     /gene="typA"
                     /locus_tag="PKI07_11055"
     CDS             1448..3283
                     /gene="typA"
                     /locus_tag="PKI07_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOC51628.1"
                     /translation="MQHIRNIAIIAHVDHGKTTLVDCLLKQSGTFRANQAIAAEERIM
                     DSMDLEREKGITIKAKNAAFKYRNYHVNIVDTPGHADFGGEVERIMNMIDGVLLVVDA
                     ADGPQAQTRFVLRKALEAGARPIVVINKIDRENANPHHVLDKVWELFLSLQASDEQMD
                     FPVIYASARDGYATTDLKHPGKTMKPLFDAILKHIPPPRVHAGDGFQLLVANLDYSDY
                     LGRIAFGKIHSGTVREGDPCVCLHGDGRKSTARITAIFQFEGLKRIQVPEAHAGDVVG
                     LTGFEDVFIGETLTDSETRPPLPFVPIDPPTIQMQFAVNDGPLAGQDGQLVTARHIWE
                     RLVKEARTNVALRIAPTAAPNIFNVSGRGEMQIAILVEQMRRENYEVLVSRPEVIYRK
                     DSQGNLLEPIEKLFLEIPREAMGAVLENLADRRGEITSIHHQGDHVSLEALIPMRGLI
                     GFETDLVNQTRGLGVLSHLFHEYGPHRGEIAARKNGSLVSMENGQAMAYALNLLQERG
                     RLIVEPGDAIYAGMIVGENARENDIPVNPCKAKKLTNMRSQGEGKGIQLNPPLKLSLE
                     RALEYIGPDEYVEATPKNLRLRKKILDEVRRKRAAQARATKGVAE"
     gene            complement(3326..3607)
                     /locus_tag="PKI07_11060"
     CDS             complement(3326..3607)
                     /locus_tag="PKI07_11060"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HOC51629.1"
                     /translation="MDLHHPILREFPEHRETIRRLQGGDDHFRNIYDEYHRLDDEIYR
                     IEEEIDFATDQEIEELKMRRAKLKDNIYRLIRRAPSGGSPAGLGTGAVG"
     gene            4027..5583
                     /locus_tag="PKI07_11065"
     CDS             4027..5583
                     /locus_tag="PKI07_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC51630.1"
                     /translation="MNLLRRKTVTALQAEALADQSLHRALGPVNLTALGVGAIIGTGI
                     FVLTGTVAAQNAGPAVVLSFALAGLASIFAALCYSEFASLVPMAGSAYTYGYATLGEL
                     FAWIIGWDLILEYAVGAITVSIGWSGYVVSFLNDCGLVVPPELSAARGTQLVQLPATL
                     AAAMKMKAGWAVFSAGLADQIKALGADPATLPQATAIFNLPAVLIIFVVTTLLVVGIR
                     ESAHFNNIIVIIKIAVVLLFIVGAARAIQTAHWHPFIPANAGTAGHFGWSGIMTGAGI
                     VFFAYIGFDAVSTAAQEARNPQRDMPIGIIGSLLICTVLYILVSAIATGVVPYAQLDV
                     PDPIAMAADHAGMGWMGGLIKLGAIAGLSSVILVMLLGQSRVFYSMSRDGLLPPFVSQ
                     VHPRFRTPWITSIITGLGVAFFSAVFTVREAGSLCSIGTLLAFVIVSVGVLVLRVREP
                     HLPRRFTTPAVWFVAPAGAISALTLMSALPLTTWLRLIIWFLIGLGVYFTYGVRRSKL
                     ASTPSTPPQP"
     gene            5743..5819
                     /locus_tag="PKI07_11070"
     tRNA            5743..5819
                     /locus_tag="PKI07_11070"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5777..5779,aa:Met,seq:cat)
     gene            6081..6746
                     /gene="infC"
                     /locus_tag="PKI07_11075"
     CDS             6081..6746
                     /gene="infC"
                     /locus_tag="PKI07_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOC51631.1"
                     /translation="MSRPYPFRSRYSREPLHRRNGKIRAREVRVLDENRQQLGVMTLN
                     EALRIAMSKGLDLVEIAPNATPSVCRIVDYGRFMYEEAKRNKDSAGRQAASKMKEIQL
                     SPTIDPHDFETKLNRAVDFLCEDMKVRIKLRFRGRQKAHTEFGFEVVNRFVKEVAPWG
                     QADSPPKMLGDRDLNAVISPLPRNKRARNPRQEASTEFPERAATGQQSESAAASANSG
                     AVS"
     gene            complement(6882..6959)
                     /locus_tag="PKI07_11080"
     tRNA            complement(6882..6959)
                     /locus_tag="PKI07_11080"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6922..6924),aa:His,seq:gtg)
     gene            complement(7289..8173)
                     /locus_tag="PKI07_11085"
     CDS             complement(7289..8173)
                     /locus_tag="PKI07_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC51632.1"
                     /translation="MKRKLILFLSQCCLVVSLGMPSLLRGETKIPDACKTGGFAIGCQ
                     AYTFNRFTVFEAIEKTAQAGGKVIEFYPGQRLSPEEPGVKWDHHASPETIQKVKDKLA
                     AARVTAVNYGVVSIPRDEAEARKIFTFAKALGLYAITTESVEAIDTIEKLVREYDLKV
                     GFHNHPKRANDPGYRMWDPNYVLSVVKDRDARIGACADSGHWVRSHLKPVECLRILKG
                     RIVSSHLKDLNEMGPGAHDVPFGTGVSDIPALLHELKVQGFAGNVAIEYEYHWENSTP
                     EVGQCIGFVRGYGTAQKW"
     gene            complement(8404..8519)
                     /gene="rrf"
                     /locus_tag="PKI07_11090"
     rRNA            complement(8404..8519)
                     /gene="rrf"
                     /locus_tag="PKI07_11090"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(8636..11571)
                     /locus_tag="PKI07_11095"
     rRNA            complement(8636..11571)
                     /locus_tag="PKI07_11095"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(11767..11843)
                     /locus_tag="PKI07_11100"
     tRNA            complement(11767..11843)
                     /locus_tag="PKI07_11100"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11807..11809),aa:Ile,seq:gat)
     gene            complement(11852..11927)
                     /locus_tag="PKI07_11105"
     tRNA            complement(11852..11927)
                     /locus_tag="PKI07_11105"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11892..11894),aa:Ala,seq:tgc)
BASE COUNT         2423 a         3753 c         3345 g         2638 t
ORIGIN      
        1 cgttcggcaa agccgggacg ggcggccagg tgttgtcgca tgccgtggtt tttcggcggg
       61 atggagccaa ggttggcgga gacgtttccc cacccgggcg gaggttgccg caaggcgact
      121 gcttgcgctg ccctcatcgc gggcagggcg ttcgcccgtt tgggtggggg ccgttgcaag
      181 gttgcgttcc ggccagaaca gcccgtgtcc ggaagcgcgc cgccgtggga gaggcgcgtt
      241 gtctcgcggg cttccattcg gacggctttg cgcgtagttg gcggaaaata tcctgggtgg
      301 ctgggggcgg catgaattgc ggggcgagag cccgggatct cgactgcgcc actggcagga
      361 aaaaggatgt ggccaccttc agccccggtt tgcggtcgaa gcatctacgc cgtgttttct
      421 ttctagttga cgggccgggg ggcaacccca actctgcaaa agattcatat gttcgcttac
      481 ttttgggtcg caacgggcgg cgcgttgggc agcgtggccc gctactggct ttcaggggtt
      541 gtggcgcaac agactggcgc cacctttccc tggggcacaa ttctggtcaa cgcctccggt
      601 tcgctggtca ttggggtctt cgcggcgctg accggcgcgg atggccgctg gctggtgccc
      661 gattggtttc gccagcagtt ctttatgctg gggctatgcg gcggctatac cactttctcc
      721 tcgttcagtt tgcaaactct caacctggca caagcgggtg aatggcttta cgcgagtgcc
      781 aatgtcgttc tttccgtcct cacctgcctg gccgccgtct ggctcgggca tggactggcg
      841 gcggccttga acgtggtttc gcgatgaccg aacagctccc acccgcgacg ccgccggcag
      901 ctccggacgg cccggttaac ccgttctcct gatatgcccc tcccccgcga agccgttttg
      961 ttgcgcatct tcattggcga aagcgatcgc tggggacacc agccgctcta tgaggcgatc
     1021 gtgctcaagg cgcgggagct gcatttggcc ggggccacgg tgttgcgcgg gccgatgggg
     1081 tttggccaat ccagccgctt gcacaccgcc aaaatcctcc gcctctcggc ggacctgccg
     1141 ctggtcattg agattgtgga ccgcccggaa aagattcagt cgttcctgcc ggtgcttgag
     1201 cccatgctgg ccggcggcct gctgaccctg gaaaaggtca ccgtgatcga gccccgggcg
     1261 gggcgggcgg ccgaatcggc gcgtccggag ccaggccctt tcgaggcttg acatttcggc
     1321 cgaaacgggc agattgcgcg ggttgattcc aaatcggagg ttgtcgtgac agccccgttg
     1381 cggcggggtc ccggctcgga ggagtcggta gcgcggttat catagcccgg tttggcgaga
     1441 ataaattatg cagcatatac gcaatattgc gattatagcc catgtggatc atggcaagac
     1501 cactttggtg gattgcctgc tcaaacaatc gggcaccttc cgcgccaacc aggcgattgc
     1561 cgccgaagag cgcatcatgg attccatgga cctggagcgg gaaaagggca tcaccatcaa
     1621 ggccaaaaac gcggcgttca aataccggaa ttaccacgtc aatatcgtgg acactcccgg
     1681 ccacgcggat ttcggcggcg aagtcgagcg catcatgaac atgattgacg gcgtgctgct
     1741 ggtggtggac gccgccgatg gcccgcaagc ccaaacccgc ttcgtattgc gcaaggcgct
     1801 cgaagccggg gcccggccca ttgtcgtgat taacaaaatt gaccgcgaaa acgccaaccc
     1861 gcaccacgtg ctcgataagg tgtgggaact cttcctttcg ctccaggcct cggacgaaca
     1921 aatggacttt ccggtgattt acgccagcgc ccgcgacggc tacgccacca ccgacctcaa
     1981 gcacccgggc aaaaccatga agccgctgtt cgacgcgatc ctcaagcaca tcccgccgcc
     2041 gcgcgttcac gccggggacg gctttcaact gttggtggcc aacctggatt attcggatta
     2101 cctcgggcga attgccttcg gcaaaatcca ctccggcacg gtgcgggaag gcgatccctg
     2161 cgtctgcctc cacggcgacg ggcgcaaaag cacggcccgg attacggcca tctttcagtt
     2221 cgagggcctg aagcggatcc aggtgcccga agcccacgcc ggcgacgtgg tcggcctgac
     2281 gggatttgag gacgtgttta ttggcgaaac gctcaccgat tcggagacgc ggccgccgct
     2341 gccgtttgtg cccattgacc cgccgaccat ccaaatgcaa tttgcggtca atgacggccc
     2401 gctcgccggg caggatggcc agttagtcac cgcccgccat atttgggaac ggctggtcaa
     2461 ggaagcccgc accaatgtcg ccttgcgcat cgcgccaacc gccgcgccca acattttcaa
     2521 cgtaagcggg cgcggcgaaa tgcaaatcgc cattctggtc gagcaaatgc gccgggaaaa
     2581 ctacgaagtc ctggtctcgc gccccgaggt catctatcgc aaggacagcc agggcaacct
     2641 gctggagccc atcgaaaaac tgtttctgga aataccccgg gaggccatgg gcgccgtcct
     2701 ggaaaacctt gccgaccgcc ggggcgagat caccagcatc caccaccagg gcgaccacgt
     2761 cagcctcgaa gcgctcattc ccatgcgcgg gttgattggc ttcgagaccg acctcgtcaa
     2821 ccaaacccgc ggcctcggcg tcctgagcca tctcttccat gaatacggcc cgcaccgggg
     2881 cgagattgcg gcccgcaaaa acggttcgct ggtcagcatg gaaaacggcc aggcaatggc
     2941 ctacgcgctg aacctgctcc aggaacgcgg ccggttgatc gtggaaccgg gcgacgcgat
     3001 ctacgctggc atgattgtag gcgaaaacgc ccgcgaaaac gacatcccgg tcaacccctg
     3061 caaggcaaag aagctcacga acatgcgctc ccagggcgag ggcaagggca tccagctcaa
     3121 tcctccgctc aagttatcgc tggaacgcgc gctggaatat atcggcccgg atgagtacgt
     3181 cgaagcgact cccaaaaacc tgcgcctgcg gaaaaagatc ctggatgaag ttcggcgcaa
     3241 gcgcgccgcc caggcccgtg cgactaaagg cgtggcggaa taacccgcgc ccccgccccg
     3301 cggcgcggcc cgcgctgacg cccggctaac caacggcgcc ggttcccaaa ccggccggac
     3361 tgccgccgga tggagcccgg cggatcaaac ggtagatatt atctttgagc ttggctcgcc
     3421 gcatcttgag ttcctcgatt tcctggtccg tggcgaaatc aatttcctcc tcgatgcgat
     3481 agatttcgtc atccagccga tgatattcat cgtagatgtt ccgaaagtgg tcgtcgcccc
     3541 cctggagtcg ccggatcgtc tcgcgatgct ccggaaactc gcgcaggatg ggatgatgta
     3601 agtccatggg agtggtctcc tgttttttgg ttttcctggt ttattcgtga aacggcgaac
     3661 cgtgagcggg tcgtccgtcg cccgcgccac gcaccgccgc ccgacggccc atcgggctgg
     3721 ccggccattc tgtcgccggc ccgggcgcgc cggcaaccgg ctgtctcgcg tttcttcaat
     3781 gccattcgcg gtctggccgc attacgcggc tcggttcgca ttctacgcga atccggcccc
     3841 cggtaaagga tttctcgctc ctctctccgg caatcgaaac gccgcgaacc ggggaaatag
     3901 cggatggtcg tggggatttc gcccctcttt ttcctccaaa ttctctgcct atcgccttga
     3961 cttccgggcg gcggcagatc attaagccga gccagtaatc gcgatgcaca atagtccggt
     4021 gacagcatga atctgcttcg acgcaaaacc gtcactgccc tgcaagccga ggcgttggca
     4081 gaccaaagcc tccatcgggc gctgggtccg gtaaatctca ccgccctggg cgtcggggcc
     4141 attatcggca ccggcatctt tgtgctcacc ggaaccgtcg cggcccaaaa tgccgggccg
     4201 gcggtggtgc tttcgttcgc gctcgccggg ctggcctcca tcttcgccgc gctgtgctac
     4261 agcgaattcg cctcgctcgt gccaatggct ggcagcgcct atacttatgg ctacgccacc
     4321 ctcggcgaac tcttcgcctg gatcatcggc tgggacctga ttctcgaata cgcggtcggc
     4381 gccatcaccg tgtccattgg ctggtcgggc tacgtcgtgt ccttcctgaa cgactgcggc
     4441 ctggtcgtgc cgcccgagct ttccgccgcc cgcggcacac aactcgtcca actcccggcc
     4501 accctggcgg cggcgatgaa aatgaaagcc ggctgggccg tcttcagcgc gggattggcg
     4561 gaccaaatca aagccctggg cgccgacccc gccacccttc cccaggccac cgccattttc
     4621 aacctcccgg cggtgttgat tatcttcgtc gtcaccacct tgttggtggt cggcattcgc
     4681 gagtccgccc acttcaacaa cattatcgtc attataaaaa tagccgtggt gctgctgttc
     4741 atcgtcgggg cggcgcgcgc catccaaacc gcccattggc atccgttcat ccccgctaat
     4801 gccggcacgg caggccactt cggctggtca ggcatcatga ccggcgcggg catcgtcttc
     4861 ttcgcctaca tcggcttcga cgccgtcagc acggcggccc aggaagcccg gaacccgcag
     4921 cgggacatgc cgatcggcat tattggctcg ctcctcatct gcaccgttct ttacatcctc
     4981 gtctcggcca ttgccaccgg cgtggtgccc tatgcccagt tggacgtgcc ggacccgatt
     5041 gccatggctg ccgaccacgc gggcatgggc tggatgggcg gcctcatcaa gctcggcgcg
     5101 attgccggcc tgtcctccgt catcctggtc atgctgctcg gccaatcccg cgtcttctac
     5161 agcatgagcc gcgacggctt gctgccgccg ttcgtcagcc aggtccatcc ccgcttccgc
     5221 acgccctgga ttacctccat catcaccggc ctcggcgtgg cgttcttctc ggccgtgttc
     5281 accgtgcgcg aagccggcag cctctgctcc attggcaccc tgctggcctt tgtcatcgtc
     5341 tccgtcggcg tgctggtgct ccgcgtgcgc gaacctcacc tgccgcgccg attcaccact
     5401 cccgccgttt ggtttgtggc ccccgctggc gccatctccg ccctgaccct gatgtccgcc
     5461 ctgccgctga ccacctggct gcgcctgatc atttggttcc ttatcgggtt gggagtctat
     5521 ttcacttatg gcgtccgccg gagcaaactc gcctcgacgc cctccacccc gccccaacct
     5581 taaaaatcac cgtgtcggcg caaatagccc ggccgatggg gaccgccgtc acgtggctac
     5641 tgggcattgg cgggcgataa gcctcggacg accttcgata tttcttcata attgtgcttg
     5701 cgctatcgcc aggattcggc atgatgagcc cacctaagaa gccgcggggt ggagcagccc
     5761 ggtagctcgt caggctcata acctgaaggt cgctggttca aatccagccc ccgcaaccaa
     5821 tttacgggcc tgaaactcac gtttcaggcc cgttgttcaa ttcttctgct cgcaatgggc
     5881 attgctatct ttacgatggt aagtgcgggt ggcgcaaaac ctgtcttgtg ggtcaactcg
     5941 gcctgcaggt ttgtgccaca ttatgcttgc cttcaggcag tgctatgtga aaataactgt
     6001 gcagtcatta accattaatc agtagcagct agtttggcag attatcaccg catgaatgaa
     6061 cttcagtcgg ggagggctaa ttgagtcgcc cctatccctt tcgctcgcgt tactcccgag
     6121 aacccctcca ccgccgtaat ggcaagatcc gcgcgcgtga ggtgcgcgtg ttggacgaaa
     6181 accggcagca gcttggagtg atgaccctca atgaagcctt gcgcattgcg atgagcaagg
     6241 gactcgactt ggtggagatt gcgcccaatg ccacgccttc cgtgtgccga atcgtggatt
     6301 atggcaggtt catgtacgag gaggccaagc gaaacaaaga ctccgccggg cggcaagcgg
     6361 cgagcaaaat gaaggagatt cagctctcgc cgacgattga tccgcatgat ttcgaaacca
     6421 agctgaacag ggctgtggat tttctatgcg aggacatgaa ggtgcggatc aaactgcgct
     6481 ttcgtggtcg tcagaaagcg cacacggaat ttgggtttga ggtcgtgaac cgcttcgtca
     6541 aggaagtcgc cccgtggggg caggcggatt cgccgccgaa gatgctcgga gaccgcgatc
     6601 tcaacgctgt gattagtcct cttcctcgca acaaacgcgc ccggaacccc cggcaggaag
     6661 cgtcaacaga gttcccggaa cgggctgcaa cgggtcagca atcggaatca gcggcagcgt
     6721 cagcaaattc gggcgcggtt tcctgagtta tcggggccta tttcaaatcc gggcccggta
     6781 accggataaa agtggcgccg gaagccgaga ctctccactt ggacgaagcg actctgtacg
     6841 aggttgggac agcagtttcg tgactattgg gagcggaaag gtggggtggc cgacgggatt
     6901 tgaacccgcg acagccagat ccacaatctg gggctctaac caggctgagc tacggccacc
     6961 aacccgcccg agcgtagatt tccacgggca gaacgtcaag tccgcagctt taaaaaaacc
     7021 ccgccgttgc cgtgtgcgac cttcctgcgg aacctaattg caacagtggg acaagcgggc
     7081 tgttttcgac gtccattgaa ggcatgccgg ccacacctcg gctacgcgtc cgtttaatcg
     7141 ttcaacttac agttcgagtt ctaagtcgct gatcacgaac agggcagggt aaattctgcg
     7201 ctgggcctgg taaagaacgc aactcctatg ccgcagtact tgtgccaatc tcaattctga
     7261 gaaacgggga gggcctcacg aatacggttc accacttttg cgccgtgcca tagccacgga
     7321 cgaagccgat gcactggccc acttccggag tcgaattttc ccagtggtat tcgtattcga
     7381 tagcgacgtt gccggcaaaa ccttgcacct tcaactcatg caaaagtgcc ggaatatcgg
     7441 aaaccccagt gccaaacggc acatcatgcg caccgggacc catctcattc aaatctttga
     7501 ggtggctgct gacaatccgt cccttgagga tgcgcaggca ctccaccggt ttgaggtggg
     7561 agcgaaccca atggccgctg tcggcacagg ctccaatacg ggcatcccgg tctttgacga
     7621 ccgataaaac gtagttcgga tcccacatgc gatatcctgg atcattggcg cgcttcggat
     7681 ggttatgaaa acccaccttg agatcatatt cccgaaccag cttctcgatg gtatcaatcg
     7741 cctcaacaga ttcggtggtg atggcataca gccccagcgc tttggcgaac gtaaaaatct
     7801 tgcgggcctc cgcttcgtcg cggggaatgc tgaccacgcc gtagttgacc gctgtgaccc
     7861 tggctgcggc gagcttgtct ttgacctttt gaatcgtttc gggagaagca tggtggtccc
     7921 acttgacacc gggttcctcc gggctaagcc gttggccggg gtagaactcg ataaccttgc
     7981 cgccggcctg agccgtcttt tcgatagctt caaagaccgt aaaccgattg aaggtatagg
     8041 cctgacagcc aatggcaaat ccgccggtct tgcaggcgtc agggatttta gtttcgccgc
     8101 gcaacaacga cggcatccct agggagacga ccaggcagca ctggctcagg aaaagaatga
     8161 gttttcgctt catacgcgtt taattaagtc tcgttagccg tcaaaggcaa taccaattcc
     8221 gagttccagg tatcaggcgc cccacgcgga gcaaagctgc cagactcggc tggccgcgcc
     8281 caaaactaac tggagacagg ctcgtgcttc ggcagaattg gaacggacgt ggcgggcgcg
     8341 cattgaaaca atctctaaga aaaaagccga accctctttt tcggagttcg gctttcataa
     8401 aagacctggc ggcgacctac tctcgcggaa tctataaaac cactaccatc ggcaagactg
     8461 cgtttgacgg ccgtgttcgg aatgggaacg ggtcggacca cagcttcaag gccaccaaga
     8521 aatttcaagg aacaccgcca accctgacgg tttctctgaa aactgcacac aggcgaacaa
     8581 ccggattaca attcgcgagt taaaatcgct cttgcccttc gaagaaggga aagaagcgat
     8641 caagccgcac gaccgattag tattagtccg ctgaacggat cactccgctt acacgcctaa
     8701 cctatcaacc cggtagtctg ccgggggtct ttaggaacct tgcggttcgg gaaacattat
     8761 cttgggatga gcttggcact tagatgcatt cagcgcttat ctcttccgca catggctacg
     8821 caacgatgcc gctgacgcga caattgccac accagaagtg cgtccaaccc ggtcctctcg
     8881 tactaaggtt ggaacccctc aagtttcctg cgcccacagt ggataaggac cgaactgtct
     8941 cacgacgttc tgaacccagc tcgcgtaccg ctttaaccgg cgaacagccg gacccttggg
     9001 accttctcca gccccaggat gcgacgagtc gacatcgagg tgccaaaccg agtcgtcgat
     9061 atgaactctt ggactcgatc agcctgttat ccctagcgta ccttttgtcc gttgagcgac
     9121 ggcaattcca cattaaaccg tcggatcatt tgggcctgct ttcgcacctg ctcgacttgt
     9181 aggtctcaca gttaacctgg cttctaccca tacgctcgac gcatagttgc caaccatgct
     9241 gagccaagct tagcgctcct ccgttactct ttcggaggaa accgccccag tcaaactgac
     9301 ccgcacgcac tgtccctcat ctggttgaca gactgaggtt agaatcctaa ttagccaaga
     9361 gtggtgtttc acattgcgac tccacgggaa ccgaggctcc cgcttcaaag tctcccacct
     9421 acgctacgca tgattaatca agacacaata cgagcttaca gtaaaggtgc atagggtctt
     9481 tccgtcctac tgcgggcagg cggcatcttc accgccgtta cagtttcgcc gagatcctct
     9541 ccgagacagt cgttcagtcg ttacacgatt cgtgcaggtc ggaacttacc cgacaaggaa
     9601 tttcgctacc ttaggaccgt tatagttacg gccgacattc actgggactt aaagaccgag
     9661 cttcgccttg cggctaacaa gatctcttaa tctttcagca ttggtcacgt gtcacaccct
     9721 atacgtcgtc ttcacgactt ggcagagtgc tgtgcttttg ttaaacagtc gccaaacgca
     9781 atttactgag gcccgccaag gctacattgc tgcaaccttt ttgggcaccc cttctcccga
     9841 agttacgggg ctaatgtgcc gagttcctta gagaggtttc tctcgcgcgc ctgagtgcgc
     9901 ttacactcac ccacctgtgt cggtttgcgg tacgggcgaa taagacatac tatgcaactt
     9961 ttctcggcaa cttctccacc ggtgcgcttc ggccgaagcc taagctcagc attcgattgc
    10021 ctagccgatg ttttccgttg cgtcatcgca tagctaattc ttacccggcg caggaatatt
    10081 taacctgctg tgcatccgct acgccttacg gcctcgcgtt agctcccggc taaccctggg
    10141 aggacgaacc tggcccagga aaccttgggt ttacggcgac caggattctc acctggttta
    10201 tcgctactcg tgtctgcatt ctcactaatc agcgctccac cgtccgtttc cgtccggctt
    10261 cgccgcgctg attacgctct cctaccactc gcaggattcg aaaatcctgc gaatccatag
    10321 cttcggtatg tgatttaatc gccaatcatt ttcggcgcga catcgctcga tgagtcagct
    10381 gttacgcact gtttaaatga tggctgcctc taagccaaca tcctcactgt ctaagcaacg
    10441 tcacatcctt tccacttaat cacattgggg caccttagct gatggtctgg actgttttcc
    10501 tctcggcaat gaaccttatc gcccactgcc taactgctgt gatactgaga tccatggcat
    10561 tcggagtttg attggttttg gtaacctggt ggggtcccta agccattcag tgctctacct
    10621 ccatggagta cttccacaac actcgccctc aagcgatttc ggagagaacc agctatcacg
    10681 gggtttgatt agtctttcgc tcctactctc aagtcatctc aggatttttc aacatccacg
    10741 agttcggtcc ttcacctggt attacccagg cttcaacctg ctcaagagta gatcacctcc
    10801 gcttcgggtc tattgcccgc gacttggtcg cgctattggc actcggtttc ccttcgcctc
    10861 cgccgcagaa cggcttaagc tggccacgga caataactcg cagactcatt atgcaaaagg
    10921 caggcagtca ccggctccat tccgaagtct ggctccggcc ttgcggccgg aacgggctcc
    10981 ggaatggagc cggctcctac acattgtagg catatggttt caggtactat ttcactccgc
    11041 tagcagcggt tcttttcacc tttccctcgc ggtactagtt cactatcggt tgccaaggag
    11101 tatttagcct tatccggtgg tccggacaga ttcatgcaga gtttcacgtg caccgcatta
    11161 cttgggatac ctctaggggg cttcgaactt cgcttacccg gctgtcacgg tctatggccc
    11221 atctttccag atggttaagc tcgctctcta cctcccacat taaggtccct caaccccggg
    11281 aagacaagtc ctcccggttt aggctgttcc gctttcgctc gccactactg acggaatcgc
    11341 ttcgttttct tttcctgggg ttactgagat gtttcacttc accccgtctc gcctcgccgg
    11401 actatgaatt catccggacg atactccggt attacccgga gtgggttttc ccattcggag
    11461 atccacggat caaagcctgc ttgcggctcc ccgtggctta tcgcaactta ctacgtcctt
    11521 catcgcctct tggcaccaag gcatccacca taagccctta gtagcttgat cacaaagtta
    11581 cctcgctgcc cccctcgaca gggagcaaca agaactcagc gaactgtaat ttgccctagt
    11641 tatctaccta tgtgcagttg tcaaagaaca ccgccgactg acttgcggcc ggagatgcag
    11701 ctccggccgg tccgggccgg caaaccgtta tgcaagtggt tttccgcgcg ccgcagcgcg
    11761 acggagtggt gggcgtgact ggagttgaac cagtgaccct gcgcttatca agcgcatgct
    11821 ctaaccaact gagctacacg cccatgaaaa ttggtggagc tgaggagatt cgaactcctg
    11881 acctatagct tgcaaagcta ccgctctacc aactgagcta cagccccact tccgaaaggc
    11941 tgaaggctga agaccgtcgc ggcaatgctt cacagcttca ggttcagcgc ctgatagagt
    12001 tcgattctct aaccacttgc caaagagcat tctgctcatt ggaaaactga actgtgcgat
    12061 aagccgagcc ttatcaactc cgcagagttg accgacctag gagcgcggtt tttaccccgc
    12121 ctccatgctt ctccttagaa aggaggtgat ccagccgca
//
LOCUS       DAOPQG010000105        12107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6093_length_12107_cov_13.925573, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000105 DAOPQG010000000
VERSION     DAOPQG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6093_length_12107_cov_13.925573"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..1083
                     /locus_tag="PKI07_11110"
     CDS             544..1083
                     /locus_tag="PKI07_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51633.1"
                     /translation="MKMPVCGVVLVALMAAGCATGNKSQEAAQHTMYNKPLTSPGAKF
                     GALPPPVQLSVLAQVGAAAVVDTVRDTSSGQVVYKVYFRESDLFPPMYIAPDGSMLNP
                     DLTVAVSARTGTRVKPEDVPPKVKKAIPEKAHSSEVAYINKEMWGERTIYVVTFKDEA
                     HNPKLLLDEEGVVVHEAQP"
     gene            complement(996..1820)
                     /locus_tag="PKI07_11115"
     CDS             complement(996..1820)
                     /locus_tag="PKI07_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOC51634.1"
                     /translation="MAPAAETLLAAARRLRAATARLRFAPPVAHVYNPLAYAWAPHAA
                     YLERFGAAPKRVVFLGMNPGPFGMVQTGVPFGEVAAVRDWLRLAGRVRRPPGEHPRRP
                     ITGFACPRSEVSGRRLWGLFAARFGTPERFFAGHIVINYCPLAFLEASGRNRTPDKLP
                     AAERDALFAACDRHLRAVLRALQPEWLIGIGEFAARRAEHVGGPGALRIGRILHPSPA
                     NPAANRDWPARVTRQLEALGVWPQARRRYGCASCTTTPSSSSSSLGLCASSLKVTT"
     gene            complement(1877..2296)
                     /locus_tag="PKI07_11120"
     CDS             complement(1877..2296)
                     /locus_tag="PKI07_11120"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOC51635.1"
                     /translation="MATLMEETAIARKTRELCQAILDEPGVQALRQSIDIFMADERAR
                     AQYDGTVAKGQALQEKQQNALALTDAEISDFEQERDALLRNPVARGFIDAQQELKEMQ
                     EFIHRHVKKTFELGRLPSAEDLAGGCCGDGHGCGCGH"
     gene            complement(2437..3075)
                     /locus_tag="PKI07_11125"
     CDS             complement(2437..3075)
                     /locus_tag="PKI07_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC51636.1"
                     /translation="MSSLRILLADDHEMIRKGLRATIESQPGWTVCGEARTGREAVAL
                     ARELRPDIVVMDFAMPELNGLEATRQIRAAHPGTEVLILTMHDSERLVHEMLSAGAHG
                     FILKTDAGEFLVAALQALARHKPYFTPKISAVVLQGYLNPETPGGQELTAREREIIQL
                     IAEGKATKEIAEALGISAKTAETHRANLMRKLDLHSTADIVRYAIRNQIVQP"
     gene            complement(3088..4665)
                     /locus_tag="PKI07_11130"
     CDS             complement(3088..4665)
                     /locus_tag="PKI07_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOC51637.1"
                     /translation="MPPAKPRRPDPAQTARKTAARRDANRGLRLLAEGRALILRATDE
                     TKLLRALCRRVVEIGGYPVAWVGRVERTPARTVRPVAWACRNGGIRTGTALRTGGPEL
                     PAAGARGGAAAARFPLKQGRRVLGVLTVQAAAPGGFRAGEKKWLAQLAGDLARGLAAL
                     RSRTAPPPAPRALRPGEQQIRLIMDNLADLVALLDVRGRRLYNSPSYRQILGDPDRLR
                     GTNSFAEVHPDDRARVTQAFRETVRTGVGQRLEYRLLDQAGRVRHIESQGSVIQDRRG
                     RVAKVLVVARDLTARKRTEEELHRLSARLLQVRDLERRHLARELHDTTAQHLAALTLN
                     LGSLKGHLDPASAAARALCDDCIQLASQAAQEIRTHSYLLHPPLLEAMGLVGAIEDYT
                     QGLSARSGITLELEAPADFGRLPEDTELALFRVLQESLSNVLKHSGSSRAQIRLARGD
                     NRVTLEVRDMGHGIPEDKLARIQARSGGSGVGLGGMQERLRLLGGQLELESGPAGTLV
                     RAAVPLPAPLPDPAAAP"
     gene            complement(4670..6988)
                     /gene="glgB"
                     /locus_tag="PKI07_11135"
     CDS             complement(4670..6988)
                     /gene="glgB"
                     /locus_tag="PKI07_11135"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOC51638.1"
                     /translation="MLLTQAELQSLVEAKQRAPHQWLGMHPLANGAGLVVRALVPQAV
                     KIEAHPLREPDQPILPLTRLHPAGLFEGVTTAVTEPYAYELVITDAQGRAQRRRDPYS
                     FAPTLSEDDLFLFGKGDERRIYDKLGAQLRVLDGAAGASFAVWAPNARRVSVVGDFNH
                     WDGRCHPMRPLGQSGVWEIFVPDVSAGAHYKFELRNARGKVMLKTDPYGFFFETAPKN
                     AAIVWNNRQFPWTDADWLARRRTRDPLRAPLSIYELHLGSWRKKSAAESPGYRALAEP
                     LIQYVKQMGFTHVEFMPVAEHAYYPSWGYQVTGFFAPTSRYGPPEDLQYLINALHAAG
                     IGVIIDWVPAHFPRDDWALARFDGTALYEHEDPRQGAHQDWGTLIFNYGRHEVANFLL
                     ANALFWCERFHVDGLRVDAVASMLYLDYSRAPGQWIPNAFGGRENLEAIEFLRRFNHL
                     VHTEFPGVMTIAEESTAWPQVTRPPYVGGLGFSFKWNMGWMHDTLGYFHRDPVHRKYH
                     QNDLSFAMLYHHHENFILPLSHDEVVHGKGSLLGRMPGDDWQKFANLRTLLGYQWLFP
                     GKKLLFMGGEFGQRAEWNANAELDWRLLTAGPYHAGLQRFLADLNQLYLAEPGLWKSD
                     YDPEGFRWVDCSDHLNSVLSFLRQEAGSPRELIVIANLTPVPRRQYRIGLPRPGKWLE
                     LLNSDAAIYGGSNLGNYGSVTATEKACHGLPCSAEFILPPLSILAFRPELRAEDAPAR
                     PAAAAAPARNAGDRPAARPGAADAAPPRPPAR"
     gene            7125..7613
                     /locus_tag="PKI07_11140"
     CDS             7125..7613
                     /locus_tag="PKI07_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOC51639.1"
                     /translation="MEALHAPWRIEYILAPKPENSESLFTRIAQANDDEANLVIARDR
                     TCFALLNAYPYTGGHLMIVPYRQVPDFQGLTDEELAGLLKLTRRCQAALSKVMKPQGF
                     NIGINLGQVAGAGVLGHLHIHVVPRWLGDTNFMPVLADTVVVPEALRELAARLRAELN
                     PS"
     gene            7692..8675
                     /locus_tag="PKI07_11145"
     CDS             7692..8675
                     /locus_tag="PKI07_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOC51640.1"
                     /translation="MPTEILHFENARLAQQLYNHDPRNLQVLEQQLGVKATSREGWIK
                     LEGEPEAIARAKHLFLLLEDSLQAGTPVRNREFSNALNIIRQEGVAMLKNILSDRIRT
                     SDKKAGVTAKTVGQKRYLDAIRTHDVTLGVGPAGTGKTYLAVAVALAALKEGKVARLI
                     LTRPAVEAGEALGFLPGDLYEKIMPYLRPLHDALHDMLPAEDIQKHTERGVIEIAPLA
                     YMRGRTLNNAFIILDEAQNSTTEQMFMFLTRLGLNSKAVITGDETQIDLPQHKHSGLL
                     EAHRALQGIEGVAIVEFTKRDVVRHPLVQRIISAYERHRGQARRPPHAEEA"
     gene            8734..9234
                     /gene="ybeY"
                     /locus_tag="PKI07_11150"
     CDS             8734..9234
                     /gene="ybeY"
                     /locus_tag="PKI07_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOC51641.1"
                     /translation="MRRGPGRRSSLTLVNRHPGREVNLPALRRIAQALLRETWPGCRF
                     DLGIYMVAAAEMARLNETFLRHSGSTDVITFDYAQSPGAGAQPGPPPALLHGEILVCL
                     DEAISQARRFRTTWQSELIRYVVHGVLHLRGYDDRSHRLRQQMKKAEDGLVRRLAGRF
                     DFGALG"
     gene            9655..12075
                     /locus_tag="PKI07_11155"
     CDS             9655..12075
                     /locus_tag="PKI07_11155"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOC51642.1"
                     /translation="MSHWTASGTWGLTTTHYGSPSHAVTDSPGTFYTNNTHSALTLAA
                     SLSLAGAVRPALGFQHRYALETGYDFGRVEVSVDGGNSWVSPELAAYTGQQGTLAREQ
                     LDLSAYAGVADFRLRFRLATDSSVVMDGWYVDDVVIAEAPAPVTLTATATNRNSIGLA
                     WTPSAAADFAAYRLYRSLTPGLDWRTARVIAEGNASATTNVTDLTASPKTTYYYRVAV
                     VNTRGLLTLGNEIAVTTHPGMDYPFTDNGEGGPNIWVAEAPWALSSEDALSPEHGWSD
                     SPGANYANNITSQALTLAAPLFLAGQASAPVFSFYHKYDFASGDSGNAEVSLNNGTDW
                     TSLASFTGTSSNQWRRARFSLAPYSNAVSVLLRFRITTDSATQGDGWHLDDISVAESP
                     AVVNAPVLDQITSHTIRVSWAAAAGDMFSHYAVFRSTTPGVGINSTLVATIPDRNLTV
                     FTDANLALDTVYYYRVYAVNSYGAFSPDSPTESSARTLNNPLPFTEDFEGSLQGWNLT
                     GTWGIATNDAHSGLACLADSPGFNYANSSDSYALTAVNLVGTTWPVLRFWDRYRLADN
                     DWARLEVSTDGNSWTPIYGAAGIRAEWAEQSIDLSPWKNQSNLRLRFHLWTDWSTTED
                     GWSIDDLTVAEHAPAALALPFYETFEAGLDQWLHASWARDTNGAYGGQYAVRDTPSGR
                     MNPDTTYWLGLGSPLNLSNAVNPQLVFWVKGQLPYRSRFRVHYSTDDGLNWGELYGLN
                     YEWDQGWTRAQVSLQSLVNRLVRLRFATWNEYGSAPAEDLWIDNLAIEESPPPVALYP
                     LTDTTSGT"
BASE COUNT         1871 a         3850 c         4256 g         2130 t
ORIGIN      
        1 agattttgcc gatattttat cccttttcac gcctaaaccc ttgattttac aggcgaaaga
       61 ggtttaacac ggttgggctt cccttcggca tcgctttagt taagtatttg gaaaaatagg
      121 ggttgggcaa ggaatcgcct tttaggcctg gtgctgcggc tggtttgggc aaaatactgg
      181 ctggacgggg cggcagggtt gtgcgggctg atgttgtttt gcccgggttg aaggggatgc
      241 ttcgattgcg gttttcggtt tcagcgcagg acacggcggg aggtcagccg ggtttccttt
      301 cgcccggttt ttttcccgga ggttgaccgg cgggcgcgga taaagcggcc gggccttttg
      361 gattcccgct tcccgggaat gaccttcatt catgccgggt ttgaagctcg cgctccggct
      421 ttgggcccgc gttctgattg ttccggttcg gcgcgggttt gcgggggatg gcgaaggcgg
      481 cgggcgccgg ggcgttatgg aagcttgcca accccggggg ggcggctaca ttggcattca
      541 cttatgaaga tgccagtttg cggagtggtg ttggttgcct tgatggcggc ggggtgcgcg
      601 accggaaaca aatcccagga ggcggcgcag catacgatgt ataacaagcc gctgacttcg
      661 ccgggggcga aatttggggc gttgccgccg ccggtgcagc tctcggtgct ggcgcaggtc
      721 ggcgcggcgg cggtggtgga tacggttcgg gacaccagct cgggccaggt tgtgtacaag
      781 gtctatttcc gcgagagcga tcttttcccg ccgatgtata tcgcgcccga tggcagcatg
      841 ctcaaccccg atctgacggt ggcggtgagc gcccgaacgg gcacgcgggt gaagcccgag
      901 gatgtgccgc cgaaggtgaa aaaggccatc ccggagaagg cgcactcttc cgaagtcgct
      961 tacatcaaca aggagatgtg gggcgagcgg accatttacg tcgtcacttt caaggacgaa
     1021 gcgcacaatc ccaagctgct gctggacgag gagggcgtcg tcgtgcatga agcgcagcca
     1081 taacggcggc gggcctgcgg ccagacgccc agcgcttcga gctggcgggt gacccgcgcc
     1141 ggccagtccc ggttggcggc ggggttggcg gggctgggat gcaggatgcg cccgattcgc
     1201 agcgcgcccg ggccgccgac gtgctcggcc cggcgggcgg caaattcacc gatgccaatc
     1261 agccattcgg gttgcagcgc gcgcaggacg gcgcgcaggt gccggtcgca ggcggcgaac
     1321 aaggcgtccc gctccgccgc gggcagcttg tccggcgtgc ggttgcggcc cgaggcttcc
     1381 agaaaggcca gggggcagta gttgatcacg atatgcccgg cgaagaagcg ttccggcgtc
     1441 ccgaagcgcg cggcgaacag gccccacaag cgccggccgc tgacctcgga gcgggggcag
     1501 gcaaagccgg tgatgggccg ccgtggatgc tcgccggggg ggcggcggac gcggccggcg
     1561 agccggagcc aatcgcgcac ggcggcgacc tcgccgaacg gcacgccggt ttggaccatg
     1621 ccgaacggcc ccgggttcat gccgagaaag accacccgct tgggtgcggc gccgaatcgc
     1681 tccaggtagg ccgcgtgcgg cgcccaggcg taggccaggg ggttatagac gtgggcgacg
     1741 ggcggcgcga agcgcagccg ggcggtggcg gcgcgcaagc gccgggcggc ggcgaggagc
     1801 gtttccgcgg ccggagccat ccgcgtgtga gccgggggtt tggccggcgt cccgggggcg
     1861 ccgggcgccg ccgggtttaa tggccgcagc cgcagccgtg gccgtcgccg cagcagccgc
     1921 cggccaggtc ttccgcggag gggaggcggc ccagctcgaa ggtcttttta acgtgccggt
     1981 ggatgaactc ctgcatttcc ttcagttcct gctgggcgtc aatgaagccg cgcgcgacgg
     2041 gattccggag cagcgcgtcg cgctcctgct cgaagtcgga gatttccgca tcggtcagcg
     2101 ccagcgcgtt ctgttgtttt tcctgcaggg cctggccctt ggcgacggtg ccgtcgtatt
     2161 gcgcccgggc gcgttcatcc gccataaaga tgtcaatgct ctggcgcagg gcctggacgc
     2221 cgggttcgtc caggatggcc tggcataatt cgcgcgtctt gcgcgcaatc gcagtctctt
     2281 ccatcagtgt tgccataagt ctaaaaatgc cgttgggccg acacgggcgc ctggccagta
     2341 agcgtgtcgc actcccagta caacatactt gccggggaaa agcaaatctc cgcgcggggg
     2401 cggggggctt tttccggccc ggccgggggg gcggggtcag ggctgcacga tctggttgcg
     2461 gatggcgtag cggacgatgt cggcggtgga atggagatcg agcttgcgca tgaggttggc
     2521 gcggtgcgtc tcggcggttt tggcgctgat gcccagcgcc tcggcgattt ccttggtcgc
     2581 cttgccttcg gcgatgagct ggatgatttc gcgttcgcgc gcggttaatt cctggccccc
     2641 cggggtttcg gggttgaggt agccttgcag caccaccgcg gagattttgg gcgtgaagta
     2701 gggtttgtgg cgggcgagcg cctggagggc ggcgaccagg aactcgccgg catcggtttt
     2761 gagaataaag ccatgcgcgc ccgccgagag catttcatgc accagccgct cggagtcgtg
     2821 catggtcagg atgagcactt ccgtgcccgg gtgcgcggcg cggatttggc gggtggcttc
     2881 caggccgttg agctcgggca tggcaaagtc catgacgacg atgtcggggc gcagttcgcg
     2941 ggccagggcg acggcttcgc ggccggtgcg ggcttcgccg cagacggtcc acccgggctg
     3001 gctttcgatc gtggcgcgca gccctttgcg gatcatttcg tggtcgtcgg ccaggaggat
     3061 gcgcagggag gacatggcgg gggggaatca gggcgcggcg gccgggtccg ggagcggcgc
     3121 ggggaggggc acggcggccc ggacgagggt gccggcgggg ccggattcga gctccagttg
     3181 gccgcccagg aggcgcaagc gttcctgcat gccgcccagg cccacgccgg agccgccgct
     3241 gcgggcctgg atgcgggcca gtttatcctc gggaatgccg tgtcccatat cgcgcacttc
     3301 cagcgtaacc cggttgtccc cgcgcgccaa gcggatttgg gcgcgggagc tgccggagtg
     3361 tttcagcacg ttggagaggc tctcctggag cacccggaag agggccagct cggtgtcctc
     3421 gggcagccgc ccgaaatcgg ccggcgcctc cagttcgagg gtgatgccgc tgcgggcgga
     3481 cagcccttgc gtatagtcct cgatggcgcc caccagcccc atggcttcca gcagcggggg
     3541 gtgcagcagg taggagtggg tgcgaatctc ctgcgccgcc tggctggcca gttggatgca
     3601 gtcatcgcac agcgcccggg ccgcggccga agccgggtcc aggtgcccct tgaggctgcc
     3661 caggttgagg gtaagcgccg cgaggtgctg ggcggtggtg tcgtgcaatt cccgggccag
     3721 gtggcggcgt tccaggtccc gcacctggag caggcgggcc gagagccggt gcagttcctc
     3781 ctcggttcgt ttgcgggcgg tgaggtcgcg cgccacgacc agcaccttgg cgacccggcc
     3841 ccgccggtct tgaatcacgc tgccctgcga ttcgatatgc cgcacgcggc cggcctgatc
     3901 cagcaggcgg tactccagcc gctggcccac gccggtccgg acggtttccc ggaaggcctg
     3961 ggtgacgcgc gcgcggtcgt cgggatgcac ctcggcgaat gagttggttc cccgcagccg
     4021 gtcggggtcg cccaggatct ggcgatagga agggctgttg tagaggcggc ggccccgcac
     4081 atcgagcagc gccaccaaat cggcgaggtt gtccatgatc aaccggatct gctgttcgcc
     4141 gggccgcaac gcccgcggcg ccggcggcgg cgccgtccgg gatcgcaggg cggcgaggcc
     4201 ccgggccagg tcgccggcca gttgggcgag ccatttcttt tccccggcgc ggaagccgcc
     4261 cggcgcggcg gcttgcaccg tgagcacgcc cagcacgcgc cggccctgct tgagcgggaa
     4321 ccgggccgcg gccgccccgc cgcgcgcccc cgccgccggg agttccggcc cgccggtgcg
     4381 gagggcggtg ccggtgcgga tgccgccgtt gcggcacgcc caggccaccg ggcgcaccgt
     4441 ccgggcgggg gttcgttcga cccggcccac ccaggccacc gggtagccgc cgatttcgac
     4501 cacccgccgg caaagggcgc ggagcagttt tgtttcgtcg gtggcgcgca ggatcagggc
     4561 gcggccttcc gcgagcaggc gcaggccgcg gttggcgtcg cggcgggccg cggtctttcg
     4621 ggcggtttgc gcggggtccg gacggcgtgg tttggcgggc ggcattgctt cagcgcgcgg
     4681 gcgggcgggg cggggcggcg tcggccgcgc cggggcgggc cgccgggcgg tctccggcgt
     4741 tgcgggccgg ggctgcggcc gcggcggggc gggcgggcgc gtcttccgcc cggagttcgg
     4801 ggcggaaggc caggatgctc agcggcggga ggatgaactc ggcggagcag ggcaggccgt
     4861 ggcaggcttt ctccgtcgcg gtcacgctgc cgtagttgcc caggttgctg ccgccgtaaa
     4921 tcgccgcgtc gctgttcagc agttcgagcc atttccccgg gcggggcaac ccgatgcggt
     4981 attggcggcg cggcaccggg gtcaggttgg cgatcacgat cagctcccgg ggggagccgg
     5041 cttcctgccg caggaaggag agcacgctgt tgaggtggtc ggagcagtcc acccagcgga
     5101 atccctccgg gtcgtagtcg gatttccaca gccccggctc cgccaggtac aattggttca
     5161 ggtcggccag gaaccgctgc aggccggcat ggtaaggccc tgccgtcaac agccgccagt
     5221 ccagttccgc gttggcgttc cattcggcgc gctgcccgaa ttccccgccc atgaagagca
     5281 gttttttgcc cgggaacagc cattggtagc ccagcagcgt ccgcaggttc gcgaacttct
     5341 gccagtcatc cccgggcatg cggcccagca gcgagccttt gccatgcacc acttcgtcgt
     5401 gggagagggg caggatgaag ttctcgtggt ggtgatagag catggcgaac gagaggtcgt
     5461 tctggtggta cttgcggtgg acggggtcgc ggtggaagta gcccagcgtg tcgtgcatcc
     5521 agcccatatt ccacttgaag gagaagccca gcccgcccac gtaggggggg cgcgtcacct
     5581 gcggccaggc ggtggactcc tcggcgatgg tcatgacgcc ggggaactcg gtatgcacga
     5641 ggtggttgaa ccgccggagg aactcgatgg cctccaggtt ttcccgcccg ccgaaggcgt
     5701 tgggaatcca ctggcccggg gcgcgcgagt agtccaggta gagcatcgag gccaccgcgt
     5761 ccacgcgcag gccgtccacg tggaagcgct cgcaccagaa cagcgcattg gccagcagga
     5821 aattcgccac ctcgtgccgc ccgtagttga agatcagggt gccccagtcc tggtgcgctc
     5881 cctggcgcgg gtcttcatgt tcgtacagcg ccgtgccgtc aaagcgggcc agcgcccagt
     5941 cgtcgcgcgg gaagtgcgcc ggcacccagt cgataatcac cccgatcccc gccgcgtgca
     6001 gcgcattgat gaggtattgc aggtcttccg gcgggccata gcggctggtc ggcgcgaaga
     6061 atcccgtcac ctggtagccc caggacgggt agtaggcgtg ttcggcgacc ggcatgaatt
     6121 cgacatgcgt gaagcccatc tgcttcacgt attggatcag gggctcggcc agggcgcggt
     6181 agccggggga ttccgccgcc gatttcttcc gccaggagcc caggtgcagc tcgtagatgc
     6241 tcagcggggc gcgcaacgga tcgcgcgtcc gccgccgcgc cagccagtcc gcgtcggtcc
     6301 acgggaactg gcggttgttc cacacaatgg ccgcgttctt cggggccgtc tcgaagaaga
     6361 agccataggg gtcggttttg agcatgactt tcccccgcgc gttgcggagc tcgaacttat
     6421 agtgcgcccc ggcggacacg tccggcacga agatttccca cacccccgac tgccccagcg
     6481 gccgcatcgg gtggcagcgg ccgtcccagt ggttgaagtc gcccaccacg ctgacgcgcc
     6541 gggcgttggg cgcccaaacc gcgaaactgg cgccggcggc gccgtccagc acgcgcaatt
     6601 gcgccccgag tttatcgtag atgcggcgct cgtcgccctt gccgaacagg aacaaatcgt
     6661 cctcgctgag ggtgggggca aaggagtacg ggtcgcggcg gcgctgcgcg cggccctggg
     6721 catccgtgat gaccagctcg taggcgtagg gttccgtcac cgccgtcgtc acgccctcga
     6781 acaggcccgc cgggtgcagg cgcgtcaacg ggaggatggg ttggtccggt tcgcgcagcg
     6841 ggtgcgcctc gattttcacc gcctgcggca ccagcgcccg caccaccagg ccggcgccgt
     6901 tggccagcgg gtgcattccc agccactgat ggggggcgcg ttgtttggct tccaccaaac
     6961 tctggagttc tgcctgtgtc agaagcatgg cgggtggatt ctagcagccg gcccggctta
     7021 ggccacacga ttttttgccc ggggcgcggg cggcgggggg aaccccggcc ggcccccggg
     7081 cgggggcttt tgacattttc cgccgtcttt actacccttg gggcatggaa gcactgcatg
     7141 ctccgtggcg gatcgagtat atcctggcgc ccaagcccga gaacagcgag agcctgttca
     7201 cgcgcatcgc ccaggcgaat gatgacgagg cgaacctcgt gattgcgcgc gaccgcacgt
     7261 gctttgcgct gctcaatgcg tatccctaca ccggcggcca cctgatgatt gtgccctacc
     7321 ggcaggtgcc ggacttccag gggctgaccg atgaggaact ggcggggctt ctgaagctga
     7381 cgcggcgctg ccaggcggcg ctgtccaaag tgatgaagcc gcaggggttc aacatcggga
     7441 taaacctggg gcaggtcgcc ggggcgggcg tgctcgggca tttgcatatt cacgtcgtgc
     7501 cgcggtggct gggggatacg aatttcatgc ccgttctggc ggacacggtg gtggtgccgg
     7561 aagccctgcg cgagctggcc gcccggttgc gcgcggaact gaacccatcc tagcccggag
     7621 cgttcgtgcc ttccgcccgc gtccagccgt ccgtttgccc gcgtcccgcc gttcacgtct
     7681 gagttcgaat catgcctacg gaaatccttc attttgagaa tgcccgcctt gcgcagcagc
     7741 tttacaacca cgacccgcgc aacctgcagg tgctggagca gcagctgggc gtcaaggcca
     7801 cctcccgcga gggctggatc aagctcgagg gcgagccgga ggccatcgcc cgcgccaagc
     7861 acctgttttt gctgctggag gattcgctcc aggccgggac gccggtccgg aaccgcgagt
     7921 tctcgaacgc gctgaacatc atccgccagg agggcgtggc catgctcaag aacatcctct
     7981 ccgaccgcat ccgcacttcc gacaagaagg ccggcgtgac cgccaagacc gtgggccaga
     8041 aacggtatct ggatgccatc cgaacgcatg acgtgaccct gggcgtgggg ccggccggca
     8101 ccggcaagac gtacctggcg gtggccgtgg cgctggccgc gttgaaggag ggcaaagtcg
     8161 cgcgcctgat cctgacccgc cccgcggtgg aagccggcga ggcgctgggg tttctgccgg
     8221 gtgatctgta tgaaaagatc atgccctact tgcgcccctt gcatgacgcc ctgcacgata
     8281 tgctcccggc ggaagacatc cagaagcata ccgagcgcgg tgtcatcgag atcgccccgc
     8341 tggcctacat gcgcgggcgc acgctcaata acgcgttcat catcctcgac gaagcgcaga
     8401 actcgaccac cgagcagatg ttcatgttcc tcacgcgcct gggccttaat tccaaggccg
     8461 taatcaccgg cgacgagacg cagattgacc tgccgcagca taaacattcc ggcctgctcg
     8521 aagcgcaccg cgccctgcag ggcattgagg gcgtcgccat tgtcgagttc accaagcgcg
     8581 acgtggtccg ccatccgctg gtgcagcgca tcatctcggc ttacgaacgc caccgcggcc
     8641 aggcgcgccg ccccccgcat gcggaagaag cctgaccccc ccggccgcgc gcgccggccc
     8701 gccggcccgc gggaggccca aacggccaaa acgatgcggc gcgggccggg ccgccgttcc
     8761 tcgcttacgc tggtcaaccg ccaccccggc cgggaagtga acctgcccgc cctgcggcgc
     8821 atcgcgcagg ccctgctgcg ggaaacgtgg ccgggctgcc gctttgatct gggcatctat
     8881 atggtggcgg cagccgaaat ggcccgcctg aacgagacct ttctgcggca ctcgggctcg
     8941 acggacgtca tcaccttcga ctacgcccaa tcccccggcg ccggggcgca accaggcccg
     9001 ccccctgcct tgctccacgg cgagatcctg gtctgcctgg acgaagcgat ttcccaggcc
     9061 cgccgctttc gcaccacctg gcagagcgaa ttaatccgct acgtggttca cggggtgctg
     9121 cacctgcggg gctacgacga ccgatcccac cggctgcgtc aacaaatgaa gaaagccgaa
     9181 gatggcctgg tccgccgact ggccggccgg tttgacttcg gcgcgcttgg gtgattccca
     9241 gccgaagccg ggctttgccg ctctgcggtt gccgcagttc aacgagggcg cgcgccgggc
     9301 cggggtgggc cgcggagccg ccagaagcgc ttccgcgaag ggcgcagcca cgccgcaaac
     9361 gcgcccgccg gaaatccttt ccggctcctc cggctccccc tcgccgggcg gggaaaaacg
     9421 aggcggctgc cggttaaaag ccgtttgttt tggctcgaca aaacggtccc cggccctatt
     9481 atttccgcac taattagaga aagctcggtt gtgccaaatt ctatgttaac gtctgttgtt
     9541 tcatctccgt tgcagtcgct gttgcgcggc attggccgtt gtctggcggg tttgctgtta
     9601 gccggttcct gttcggcggg gacacttccc ttctaagatg attttgaaag cggtttgtcc
     9661 cactggacgg cgagcggcac ctgggggctg accaccacgc attatggctc gcccagccac
     9721 gccgtcaccg acagcccggg cacgttctat accaataata cccactcggc cctgaccctg
     9781 gcggcatcgc tttcgctggc cggcgccgtt cggccggcgc tcggtttcca gcatcggtac
     9841 gcgctggaaa cgggctacga tttcgggcgg gtggaggttt ccgtggatgg cggcaatagt
     9901 tgggtttcgc cggagctggc cgcgtacacg ggccagcagg ggaccctggc gcgcgagcag
     9961 cttgatcttt ccgcctatgc cggggtcgcg gatttccgcc ttcgtttccg cctggccacg
    10021 gacagttccg tggtgatgga cggctggtat gtggatgacg tggtcatcgc tgaagccccc
    10081 gcgcccgtga cgctgaccgc cacggctacc aaccggaatt ccatcggcct ggcctggacg
    10141 ccgtccgcgg cggcggactt tgccgcgtat cgcctctacc gttctttgac gcccggcctt
    10201 gactggcgca cggcgcgggt gattgccgag gggaacgctt ccgccaccac caacgtgacc
    10261 gacctgacgg cctcgcccaa gacgacgtat tattaccggg tcgcggtggt caacacccgg
    10321 ggcctgctca ccctgggcaa tgaaatcgcc gtcaccacgc atcccgggat ggattatccg
    10381 tttaccgaca acggcgaggg cggccccaac atttgggtcg ccgaggcgcc ctgggcctta
    10441 agttccgagg acgccttgtc gccggagcat ggctggtcgg attcgcccgg cgccaattac
    10501 gccaacaaca ttacctcgca ggcgctcacg ctggcggcgc cgctctttct ggcgggccag
    10561 gccagtgcgc ccgtcttctc gttctatcac aagtatgact ttgcctccgg ggactcgggc
    10621 aatgcggaag tatcgctcaa caacggcacg gactggactt ccctggccag tttcaccggc
    10681 acttcgtcca accagtggcg gcgcgcccgg ttcagcctgg ccccctactc caatgcggtc
    10741 tccgtgttgt tgcgcttccg gatcaccacg gattcagcca cccaaggtga tggctggcac
    10801 ctggacgaca tctcggtggc ggaatcgccc gccgtggtca atgcgccggt gcttgaccag
    10861 atcacgtcgc ataccatccg cgtcagttgg gcggccgctg ccggggatat gttctcgcac
    10921 tacgccgtgt ttcgctccac gacgccggga gtcgggatca actcgacgct ggtggcaacc
    10981 atccccgatc ggaacctgac ggtgttcacc gacgccaacc tggccctcga caccgtttat
    11041 tactaccggg tctatgcggt gaattcatac ggcgccttct cacccgacag ccccacggaa
    11101 agcagcgcgc gcacgttgaa taatccgttg ccgttcacgg aagattttga aggctcgctg
    11161 caaggatgga acctcaccgg cacctgggga atcgcgacca acgacgccca ctccggcctg
    11221 gcctgcctcg cggattcgcc cggctttaat tatgccaaca gttcagacag ctacgccctg
    11281 acggcggtga acctggtggg cacgacctgg ccggtattgc ggttttggga tcggtatcgg
    11341 ctggcggaca acgactgggc ccggctggag gtgtccacgg atggcaacag ttggacgccg
    11401 atttacgggg cggcgggcat ccgcgccgag tgggccgagc agagcattga cctgtcgccc
    11461 tggaagaacc agagcaacct gcggctgcgg tttcatctgt ggacggattg gagcacgacc
    11521 gaggatgggt ggagcattga cgatttgacg gtggcggagc atgccccggc ggccctggcg
    11581 ctgcctttct acgagacctt cgaggcgggg ctggaccagt ggctgcacgc gtcctgggcg
    11641 cgggacacca acggggctta tggcgggcag tatgcggtgc gggacacgcc gagcgggcgg
    11701 atgaacccgg acacgaccta ttggctgggg ctggggagcc ccttgaacct gagcaacgcg
    11761 gtcaacccgc aactggtatt ctgggttaaa gggcagttgc cgtatcggtc gcggttccgg
    11821 gtgcattact cgacggacga cgggttgaac tggggcgagc tctatgggct caactatgag
    11881 tgggatcagg gctggacgcg ggcgcaagtg tcgctgcaaa gcctggtcaa ccggctggtg
    11941 cggctgcggt ttgccacctg gaatgaatac ggcagcgccc cggctgagga cctttggatt
    12001 gacaacctcg cgattgagga atcgccgccc ccggtggcgc tttatccgtt gactgatacc
    12061 acttctggca cgtaaatgca ctattctccg ctgcggtagc gtttact
//
LOCUS       DAOPQG010000106        11977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6161_length_11977_cov_15.631857, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000106 DAOPQG010000000
VERSION     DAOPQG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6161_length_11977_cov_15.631857"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..426
                     /locus_tag="PKI07_11160"
     CDS             196..426
                     /locus_tag="PKI07_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51643.1"
                     /translation="MLAQQIEFDLAVPICAWCKPREAGDALVALSHGICPRHLRQIKR
                     ELHRRAGAAPRRRARRTRPPSEPEAELLQLSS"
     gene            507..1268
                     /locus_tag="PKI07_11165"
     CDS             507..1268
                     /locus_tag="PKI07_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOC51644.1"
                     /translation="MNRTLIVVPTYNERENLLPLAQRLLALPVPVDLLVVDDNSPDGT
                     GKLADELAAKHPSIHVLHRTEKSGLGRAYIAGFKWALARDYEFVFELDGDFSHNPDDI
                     PMFLEAARNADLVLGSRYMNGIRIINWPLSRLMLSKCAAKYVQMITGMPITDPTGGYK
                     CFRRRALEASRLDEIHSNGYSFQIEMTHKLWRQGLRVVEVPIIFTERFHGHSKMSGNI
                     ISEAFIMVWRLLIQNKFRRRPRPLPSPSPRGVLPS"
     gene            1466..4912
                     /locus_tag="PKI07_11170"
     CDS             1466..4912
                     /locus_tag="PKI07_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOC51645.1"
                     /translation="MKRLAILSSAAAFLLAALPARALIDAALQMQLGNPSGATADTNN
                     YHHYLIQRPVEALDYNATRGLPNWVSWNFTSADVSNAVARSPVYFTDTNLPPNFYRVT
                     SADYAGSGWDRGHLCPSADRLNSRENNDMVFLMSNIMPQASSQNSGVWAQLEGDCRKI
                     ASTNNEMLIICGPSQFTGDTIASGRVAVPGHTWKIAVVVPLGSGSALDRIDYSTRVIA
                     VTIPNSNSVGSTPWTNFLTSVNELETETGFTFFTALPPNLAAVLRSKVDGQLPPAPAI
                     AGFWPASGSAGASISIVGTNLNFTTNVTFGGVSASFLIHSSTNLSAIVPAGASSGPIV
                     AATLGGTAAGPGAFIVSTNDAPDLAIAITRSGDFTQGDANAAYTIIVTNLGPGASSGV
                     VAVTNLLPAGLSAVGWGGPGWTADLGTLTCTRADSLPPGSAYPPITLTVKVAANAPAC
                     VTNMAVVSGVGDPYADNNTALEVTIINPAAAPTVVTGIATGVGTTTATLNGIVNPNGQ
                     PAQVHFEYGPTTNYGATAFCAGTYSGMTPQAVSADLEGLLAGATYHFRVVATNVLGAT
                     AGADQTFTTGGPDLAVSVAHHGDFVQGYICSYILTVTNLGDGASLGAVTVTNTLPAGL
                     TACGLSGDGWETDLSTLTCTRADSLAAGAAYPAIILSALVATNAPASVTHMAAVSGGG
                     ENNLDNNTAGDPTTINPAGGEAGGSILAGWNVSALSNYGPSPLAPTTNAAHLAVEGLT
                     RGAGVGTGGTAAARAWGGTTWTNASAAAAISSDRCATFSVAASNGYTLSFAAISRFDY
                     RRSPTGPTNGLLQCQLGTGDFITLAAVSYPSAGSGGASLSPILLSSIPALQNIGPGTN
                     VTFRIVNWAGTSSAGTWYIFDVANSAAPDFEVLGTVAPAVTPAADLAIHLTHAGNFTQ
                     GDLGRTCTLLVTNCGTAPTTGAVMVTNLLPAGLVATAIGGSGWASDLGALACSRSDAL
                     AAGASYPPITVTVNVATNAPASLTNLAVVAGGGETNMANNVAHDPINIIALAPIELWR
                     LHWFGTTANSGPAADTAVAAGDGLPNLLKYALGLNPLVPADDPVAGDIASGYLRLTVP
                     KNPDAVDVTFHVEAAAALDAAWTTNNTTVEINTATLLRARANDPVAGSAAGFIRLRIS
                     RP"
     gene            4989..5429
                     /locus_tag="PKI07_11175"
     CDS             4989..5429
                     /locus_tag="PKI07_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51646.1"
                     /translation="MKKVYVLGLALLTWGGCQTRPLLHDVSPRVIGRVVAADTGRPLA
                     GVQVINYAQPEDYDSASPPKGGQLLKRKPIARTDSEGRFIFAREQVLTPFGGSGWFSL
                     QLFINQAGYERFLTNYSYLNLGTNTWKGQPVLDTGDIRLQPAAR"
     gene            complement(5460..6170)
                     /gene="radC"
                     /locus_tag="PKI07_11180"
     CDS             complement(5460..6170)
                     /gene="radC"
                     /locus_tag="PKI07_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOC51647.1"
                     /translation="MNGTRLKDQPASERPRERLVEKGADALSHAELVAILLRTGLRGR
                     NAVEIGRELLAQQGSLHALARASLADLQQIKGIGRTKAVTLAAAFTLARKMAEELRHE
                     SPLLDTPEAVAALMREDARLRSVEAFQVLLLNTRRRLINVVKIADGTLDTILVHPRDV
                     FKPAIAANAAAVVLIHNHPSGEPNPSEADIKVTRDLIRAGQLLKIEVLDHVILGRATR
                     ERPKDYMSLRELGYFAGY"
     gene            complement(6235..7125)
                     /locus_tag="PKI07_11185"
     CDS             complement(6235..7125)
                     /locus_tag="PKI07_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOC51648.1"
                     /translation="MRTCVIFNPAARGDKARHFRRHLGAIGSGSSLRQTAGAGDARRL
                     AAEAVGEGFETIVAAGGDGTLNEVLNGLGDAPEGFARARLGILPLGTVNVFARELALP
                     MRLEAAWQSIRAGRETRIDLPAVEHRISGAAALRYFAQLAGAGLDARAIELVHWPLKK
                     KIGPLAYVLAGLKALAGPQSQITVAGGGRTATGELVLIGNGRLYGGDYPIFPQANLRD
                     GLLEVCLFPRVNWLTLARCAPALLRRKKLPASVALTFQAESLTLASPSPTPLEADGEL
                     IGSLPAAFSLRRGSLRVIVP"
     gene            7243..8151
                     /gene="grpE"
                     /locus_tag="PKI07_11190"
     CDS             7243..8151
                     /gene="grpE"
                     /locus_tag="PKI07_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOC51649.1"
                     /translation="MSELSAPKLSKWPFLLGDALLLALAVFIACQRGFASGVVEVTLL
                     VLCVAGAALLGITPYLLEYGALVKVVEAGALTSVTAQIKDLESIAARIGGATGRWQDA
                     QDAADQTARAAREIADRMSSEVRGFTEFMQKANDSERATLRLEVEKLHRAEGEWLQVL
                     VRVLDHVYALNAGARRSGQPNLIEQLSHFQNACRDAARRVGLTPFAAEAEEPYDAQRH
                     QLLDGAPPPPADAVVAETIATGYTFQGRLIRPALVHLREPAAPAASAPAEAAGDSQAQ
                     FALAPEPPAVSPAAAAEPDPETPGVA"
     gene            complement(8199..10064)
                     /locus_tag="PKI07_11195"
     CDS             complement(8199..10064)
                     /locus_tag="PKI07_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC51650.1"
                     /translation="MIYTRINARRLRLILAASFALSLFCPATLPAARPPRKAAVVLLE
                     AEQFADPGGWVLDPQFMDLMGSPYLLAHGLGEPVRDASTPARFPAAGAYRVWVRTRDW
                     VAPWKAPGAPGRFQVLIDGQPLATTFGTEGAAWHWQDGGMVKVGREARVALHDLTGFG
                     GRCDALLFCRDPDFTPPNELAALTAFRRQALGLPAAPENGGRYDLVVVGGGIAGTCAA
                     LSAARLGLSVALVQDRPVLGGNNSSEVRVWLGGGINLPPYPRIGDIVAELEPKVRAQV
                     GTNNTAEIYEDDRRLALVRSERNLALFLEQRVNGVETVGGVLRAVVAQDIRSGRRTRF
                     SARWFADCTGDGAIGALAGADADITETGHMGPSNLWHVRDTGQPQPFPACPWAVDLRD
                     KPFPGRRLPGQAPEKDPLSQLGGWFWESGFYRHPITDVEWMRDLNWRAMYGAWDTLKN
                     VDKLYPNHKLNWAAYIAGKRESRRLLGDVILTGDDVRTNRLFPDACFPCTWSIDLHLP
                     NPAYAKGHEGAEFIATANHGKYRAPYWAPYRSLYSRNLTNLFMAGRDISVTHEALGAV
                     RVMRTCGTMGEIVGMAASLCKRHDCQPRGVYERHLDELKQLMTRGVGKHATQTVP"
     gene            complement(10069..11487)
                     /locus_tag="PKI07_11200"
     CDS             complement(10069..11487)
                     /locus_tag="PKI07_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOC51651.1"
                     /translation="MNYLLPGRLVLLAALLWLGSAAAAPRPNMLIFLADDLGYADVGF
                     NGAKDIRTPNLDRLAAAGARLEQFYAQPLCSPTRAALMTGRYPMRYGMQVGVIRPWDQ
                     RGLPLTERLLPQALREAGYTTAIVGKWHLGNFERACLPTARGFDHQYGHYCGAIDYFT
                     HERDGGFDWHRDDRVCRDEGYSTHLIAREAQRLLREQAADKPFFLYVPFNAVHAPHQV
                     PEKYTVPYAHLPQPRRAYAGMVAAMDEAIGQILATLDARGFRTNTLIFFASDNGGPNP
                     GRVSHNGPLRAGKGTVYEGGARVCACAAWPGRIPAGASIPQPLHMVDWYPTLLKLAGA
                     PLEPSRRLDGHDLLAVLTRGQPSPHDAILINAAPRGGAIRVGDWKLVLNGAAGVGDGE
                     SPGPAPKKGKRAARTRPPSQVELFNLVRDVSEKDNLAPEHPEKVKELRARYDAFARQA
                     VAPGNQPKPADYRAPRIWGQPD"
BASE COUNT         1969 a         3956 c         4023 g         2029 t
ORIGIN      
        1 tggggttggc ggtggtggac gaggcccgtt gttattgacg ccgaagtttc tgcagatgcg
       61 gaggctggga tgaaagcgtc tattgattta cagcgcccag ccgcccctga gggcaacggt
      121 ccgccagttt tcgccggaga ttttccgagg cgggcggctt gacaagccgg cacggtttcc
      181 cacattaaga gccggatgtt ggcacagcaa atcgagtttg acctggcagt gccgatctgc
      241 gcgtggtgca agccgcgcga ggccggcgac gccctcgtgg cgctatcgca cggcatttgc
      301 ccgcgtcacc tgcgccagat caaacgcgaa ttgcatcggc gcgcgggcgc ggctccgcgg
      361 cggcgcgccc gtcgaacccg gccgcccagc gagccggaag ccgagctcct gcagttgtcc
      421 agttagcggt ttcgcgcgcc cggcggcgag gaatagtcct tgtttttgat ggcaaggtcg
      481 cttatcaatc agggggcctc gaacccatga accggacctt aatcgttgtg ccgacttata
      541 acgagcggga gaatctgctc ccgctggcgc aacgattgct tgcgcttccg gttccggtgg
      601 acttgctggt ggtggacgat aattcgcccg acggcacggg caagctcgcg gatgagctgg
      661 cggccaagca tccgtccatt cacgtcctgc atcgcaccga aaagagcggt ctggggcggg
      721 cgtacattgc cggcttcaaa tgggcgctcg cgcgcgacta tgagtttgtc ttcgagctgg
      781 acggcgactt ttcccacaac ccggacgata ttcccatgtt tctcgaagcg gcccggaatg
      841 ccgacctcgt gctgggctcg cgctatatga acggcatccg catcatcaac tggccgttga
      901 gccgcctcat gctcagcaag tgcgcggcca agtatgttca gatgatcacc ggcatgccga
      961 ttacggaccc gacgggcggc tataagtgtt tccgccgccg cgcgctcgaa gccagccggc
     1021 ttgatgaaat ccattccaac ggctacagct ttcagatcga gatgacccac aagctctggc
     1081 gccaggggct gcgcgtggtc gaggtgccca tcattttcac cgagcggttt cacggccatt
     1141 ccaagatgtc cggcaacatc attagtgagg cgttcatcat ggtctggcgc ctgttgatcc
     1201 agaataagtt ccgccgccgg ccgcgccccc tcccctcccc ctccccgcgg ggcgttcttc
     1261 catcttaggc cgcgccttaa cgcgagggct ggcgccactc catgccttcg gcgggaaggg
     1321 gatggtgatt gccaagccgg gcgggaaatc gtatatataa ttatgcccag cctcgtggcc
     1381 actttgagca gcgcaagcga gcgcgtcgtg aaccgtgccc ggcctttttt gccgggtgaa
     1441 aatgaccgcg cctaagcggt atccgatgaa acggttggca attctctcca gtgcggcggc
     1501 gtttttgctg gcggcccttc cggcgcgcgc ccttattgac gccgcgctgc aaatgcagtt
     1561 gggcaacccc agcggcgcca cggccgacac caacaattat caccactact tgattcagcg
     1621 cccggtcgag gctctggatt acaacgcgac gcgcggcctc cccaactggg tgagttggaa
     1681 ctttacgtcg gcggatgtga gcaacgcggt ggcgcgatcc cccgtgtact ttacggatac
     1741 caacctgccg cccaatttct atcgggtgac ctccgccgat tacgcggggt ccggctggga
     1801 tcggggccat ttgtgtccct cggcggatcg cctcaacagc cgggaaaaca atgacatggt
     1861 gttcctgatg tcgaatatta tgccgcaggc ttccagtcag aactccgggg tctgggcgca
     1921 gttggagggc gattgccgca aaatcgcgag caccaacaac gagatgctga ttatctgcgg
     1981 ccccagccag tttaccggcg acaccatcgc ttcggggcgg gttgcggtgc cggggcacac
     2041 gtggaagatt gccgtcgtgg ttcccctggg cagcggctcc gcgctggatc ggattgacta
     2101 ttcgacgcgc gtcatcgcgg tgaccattcc caattcgaac tccgtgggca gcacgccctg
     2161 gacaaatttc cttacctcgg tcaatgaatt ggaaacggaa accgggttta cgttctttac
     2221 ggcgctgccg cccaacctcg ccgcggtgct gcggagcaaa gtggacggcc aactgccgcc
     2281 ggccccggcc attgccgggt tctggccggc cagcggcagt gcgggcgcca gcatcagcat
     2341 cgtcggcacg aatctcaact tcaccaccaa cgtgacgttt gggggcgtca gcgcgtcatt
     2401 tctcattcat tccagcacca accttagcgc catcgttccg gcgggggcga gttcggggcc
     2461 catcgtggcc gcgaccctgg gcggcaccgc cgccggtccc ggcgccttca tcgtcagcac
     2521 caatgacgcg ccggatctgg ccattgccat cactcgctcg ggggatttca cccaggggga
     2581 tgccaacgcc gcctatacca tcatcgtcac gaacctgggg ccgggcgctt ccagcggcgt
     2641 cgtggccgtc acgaatctac tgccggccgg gctgagcgcc gtcggttggg gtggtccggg
     2701 ctggacggcg gacctcggca cgctgacctg cacgcgcgcg gacagcttgc cgcccggcag
     2761 cgcgtatccg ccaatcacgc tcaccgtgaa ggtggcggcc aacgcgcctg cctgcgtcac
     2821 gaatatggcc gtcgtctctg gcgttggcga cccctatgcg gacaataaca cggcgctgga
     2881 ggtgacgatt atcaacccgg ccgcggcgcc gacggtcgtg acgggcatcg ctactggcgt
     2941 gggcacgacc acggccaccc tgaacggcat tgttaatccc aatggccagc cggcccaggt
     3001 gcatttcgag tatgggccga ccaccaacta tggcgcgacg gcgttttgcg cgggcactta
     3061 cagcggcatg acgccccagg cggtcagcgc ggacctcgag ggactgctgg ccggggcaac
     3121 gtatcatttc cgggtggtgg ccaccaatgt cctcggcgcg accgcgggcg cggatcagac
     3181 cttcaccacg ggagggccgg acctggcagt ttctgtggcc catcatggcg attttgttca
     3241 aggctatatc tgcagctata ttctgacggt gaccaacctg ggcgatgggg cttcgctggg
     3301 cgcggtcacg gtcacgaaca ctctgcccgc cgggttgacg gcttgcggcc tcagtggcga
     3361 tgggtgggaa accgatctca gcacgctcac ctgcacccgc gcggacagcc tggccgccgg
     3421 cgccgcttat ccggcgatta tcctcagcgc gctggtggcc accaatgcgc ccgccagcgt
     3481 cacccacatg gcggcggttt ccggcggcgg cgaaaacaat ctggacaaca acacggccgg
     3541 cgacccgacg accattaacc ctgcgggtgg cgaagcgggc gggagcattc tggccgggtg
     3601 gaacgtgagc gcgctgtcga attacgggcc ttcgccgctg gcgcccacga cgaatgcggc
     3661 ccacttggcg gtggagggat tgacccgggg ggcgggcgtt ggcacgggcg ggaccgcggc
     3721 ggcgcgggcc tggggcggca ctacgtggac caatgcctcc gcggcggcgg ccatcagttc
     3781 tgatcgctgc gccaccttta gcgtggcagc cagcaatggc tacaccctgt cttttgccgc
     3841 gattagcagg ttcgattatc ggcgctcgcc aaccgggccc accaatggct tgttgcagtg
     3901 ccagctcgga acgggtgatt tcatcaccct ggccgcggtg agctatccat cggcgggcag
     3961 cggcggggcg tccttgagcc cgattctcct gtcatccatc ccggcgttgc agaatatcgg
     4021 ccccggcacc aacgtcactt tccgcatcgt caactgggcg ggcacgtctt ccgccggcac
     4081 ctggtatatc tttgacgtgg cgaactccgc cgcccccgac ttcgaggtcc tggggacggt
     4141 ggcgccggcg gtgacgcccg cggccgacct ggccatccac ttgacgcacg cgggcaattt
     4201 cacccagggc gacctggggc ggacctgcac cctcctcgtt acgaactgcg gcacggcgcc
     4261 caccaccggc gcggtgatgg tgacgaacct gctgccggcg gggctcgttg ccaccgccat
     4321 cggcggcagc ggctgggcca gcgacctggg cgcgctggcg tgctcgcgct ccgatgcgct
     4381 ggctgcgggc gccagctatc cgccgattac cgtgaccgtg aacgtggcca ccaacgcgcc
     4441 cgccagtctc accaacctgg ccgtggtggc gggcggcggg gaaaccaaca tggcgaacaa
     4501 cgtggcgcat gacccaataa acattattgc cctggctccc atcgagctct ggcgattgca
     4561 ttggtttggc accaccgcca acagcggccc ggcggcggac acggcggtgg ccgccggcga
     4621 cggcctgccc aacctcctca agtatgccct gggcctcaat ccgctcgtgc ctgcggacga
     4681 cccggtggcg ggggatattg ccagcggcta cctgcgtctc accgtgccga agaatccgga
     4741 cgccgttgac gtcaccttcc atgtggaagc ggctgccgcg cttgacgcgg cctggaccac
     4801 caacaacacg acggtggaaa tcaataccgc aaccctgctc cgggcgcgcg ccaacgatcc
     4861 ggtggcgggc tccgccgccg ggtttatccg cctgcgcatt tcccgtccct gaccgccgct
     4921 cccggcccgg gggccgatgc ccggtgggaa tctttgcgtt agggcggcga agctgttagg
     4981 ctggggccat gaagaaagtc tatgtgctgg gcctggcctt gctgacctgg ggaggatgcc
     5041 agacgcgccc gcttttgcac gacgtgtcgc cccgcgtaat cgggcgcgtg gtggccgctg
     5101 acacgggccg gccgctggcc ggggttcagg tcattaacta cgcgcaaccc gaggactacg
     5161 attcggcgtc cccgcccaag ggcggtcagc ttctcaagcg caagcccatt gcgcggaccg
     5221 actcggaggg gcggtttatc tttgcgcgcg agcaggtatt gacgcccttt ggcgggtcgg
     5281 gctggttttc cctgcagctt ttcatcaacc aggccggcta cgagcggttc ctcaccaatt
     5341 attcctacct caacctcggc accaatacct ggaaaggcca gccggtcctg gacacgggtg
     5401 atattcgcct gcagccggcg gccaggtaag cgcagccccg cccgcccgcc ggagcggctt
     5461 cagtacccgg caaaataacc caactcgcgc agggacatgt agtctttggg ccgttcccgg
     5521 gtggcgcggc caaggatgac atgatcgagc acctcaatct tcagcagttg gccggcgcgg
     5581 atcaaatcgc gggtgacttt gatgtccgcc tcggacgggt tgggctcgcc ggagggatga
     5641 ttatgaatca gcaccacggc ggcggcgttg gcggcgatgg ccggtttgaa cacgtcgcgg
     5701 ggatggacca ggatggtgtc gagggtgccg tcggcgattt tcaccacatt aatgaggcgg
     5761 cggcgcgtgt tgagcaggag aacctgaaag gcttccacgc tgcgcaggcg ggcatcctcg
     5821 cgcatgagcg cggcgacggc ttcgggcgtg tcgagcagtg gcgattcgtg gcgaagttcc
     5881 tccgccattt ttcgcgccag ggtgaacgcg gcggccagcg tcaccgcctt ggtgcggcca
     5941 atgcctttga tttgctgcag gtcggccagc gaggcccgcg ccagcgcgtg cagggaaccc
     6001 tgttgggcca gcagctcccg gccgatttcc acggcgttgc ggccccgaag cccggtgcgc
     6061 agcaaaatgg cgaccaattc agcatggctg agggcgtcgg ctcccttttc gaccagccgc
     6121 tcgcgcgggc gttcgctggc gggttgatcc ttcaggcgtg tgccgttcat gggaagcaac
     6181 tgtagggcgg gcggggccaa aagcacgcaa aaagtcgccg cgccaccggc cggctcaggg
     6241 gacaattacg cgcaagctgc cgcggcgcag ggaaaaggcg gcgggcaaag agccgatcaa
     6301 ttcgccgtcg gcttccagcg gggttggcga agggctggcg agggtgaggg attcggcctg
     6361 gaaagtcagc gcgacggacg ccggcagctt cttgcgccga agcagggccg gggcgcagcg
     6421 ggcgagggta agccagttta cgcgcgggaa gaggcatact tccagcagcc cgtcgcgcag
     6481 attcgcctgc gggaaaatgg ggtaatcccc gccatacaga cgcccgttgc cgataagcac
     6541 cagttcgccc gtggccgtgc gccccccgcc cgccaccgtg atttgcgact gggggccggc
     6601 cagggctttt agcccggcca gcacgtaggc cagcgggcca atcttcttct tcagcggcca
     6661 gtggacgagt tcgatggccc gcgcgtcgag gccggcgccg gccaactgcg cgaagtagcg
     6721 gagcgcggcg gccccggaga tgcggtgctc cacggcgggg aggtctatcc gcgtttcgcg
     6781 tccggcgcgg atgctttgcc aggcggcctc cagccgcatg ggcagcgcca gttcccgggc
     6841 gaatacattg accgtgccca acggcaggat gcccagccgc gcgcgcgcga acccctcggg
     6901 cgcgtcgccc aggccgttga ggacttcatt gagcgtgccg tcgcccccgg cggcgacaat
     6961 ggtctcgaag ccttcgccga ccgcctccgc cgccagtcgg cgggcatcgc cggcccccgc
     7021 ggtttgccgc agggagcttc cgctcccgat cgcgccgaga tggcgccgga aatgccgcgc
     7081 cttgtcgccc cgggcggcgg gattgaagat gacgcaggtg cgcacgcctg attgtttgcg
     7141 gggacgcgcg cggcgagtcg agccgaaagg tgccgaactt tcccgttcgg gcggcgcgcc
     7201 gatgcttgaa atccgggccg gcttgctcta catcttgcgg ctatgagcga gttgagtgcg
     7261 ccgaagctgt ccaagtggcc ctttttgctg ggcgacgccc tcctgctggc gctggctgtt
     7321 tttattgcct gccagcgcgg gttcgcgtcc ggggtggtgg aggtcacact cctggtcctg
     7381 tgcgtcgcgg gcgccgcgct gctcggcatc acgccgtacc tgctcgagta tggcgccctg
     7441 gtgaaagtgg tggaagcggg cgccctgacc tcggtgaccg cccaaatcaa agacctcgaa
     7501 agcattgccg cccggatcgg cggcgccacc gggcgctggc aggacgcgca ggacgcggcg
     7561 gaccagaccg cgcgggcggc ccgggaaatt gccgaccgca tgagcagcga agtgcggggg
     7621 tttaccgaat tcatgcagaa ggccaacgac agcgaacggg ccacgctgcg gctcgaagtc
     7681 gagaagctcc atcgcgccga aggcgagtgg ctgcaggtgc tggtgcgggt gctcgaccat
     7741 gtgtatgccc tcaatgccgg cgcccgccgc tccggccagc cgaacctgat tgagcaactg
     7801 tcccacttcc agaacgcctg ccgggacgcc gcccggcgcg tcggcctgac tccgttcgca
     7861 gcggaggcgg aggaacccta tgatgcccag cgccaccagt tgctcgacgg cgcccctccg
     7921 ccgccggcgg atgccgtggt cgccgaaacc atcgcgaccg gctacacgtt tcaggggcgg
     7981 ctcatccggc ccgcgctggt gcatttgcgt gaacccgccg cgcccgcggc ttccgccccc
     8041 gccgaggcgg cgggggacag ccaggcccaa ttcgcgctgg ctcccgagcc tcccgcggtc
     8101 agccccgccg ccgccgccga gcccgatccg gaaacgcccg gcgtggcgta aaagcggcaa
     8161 cccggcccgc gcccgaaggg cggcgcgttc gtgtccgctt acggcaccgt ctgggtggcg
     8221 tgcttgccca cgccccgggt catgagctgc ttgagttcgt cgaggtgccg ctcataaacg
     8281 ccccgcggct ggcaatcgtg ccgtttgcag agggaagcgg ccatcccgac gatctcgccc
     8341 atagtgccgc acgtgcgcat gacgcgcacc gcccccagcg cctcgtgggt gacgctgatg
     8401 tcccggccgg ccatgaagag gttggtcaga ttgcggctat acagcgagcg gtagggcgcc
     8461 cagtaaggcg cccggtattt gccgtggttg gccgtggcta taaactccgc gccttcgtgc
     8521 cctttggcgt aggcgggatt gggcaggtgg agatcaatgc tccaggtgca gggaaaacag
     8581 gcatccggga acaggcggtt ggtgcggacg tcatcgccgg tgagaatgac atcgcccagc
     8641 aggcggcgcg attcccgctt gccggcaatg taggcggccc agttcagctt gtggttcggg
     8701 tagagcttat ccacgttttt aagggtgtcc caggcgccat acatggcgcg ccagttcaga
     8761 tcgcgcatcc actccacgtc ggtgatcggg tggcgataaa agccgctttc ccagaaccag
     8821 cctccgagct ggctcaacgg atctttttcc ggcgcctggc ccggcaatcg ccggccgggg
     8881 aaaggcttgt cgcgcaaatc caccgcccag gggcaggccg gaaagggttg cggctggccg
     8941 gtgtcgcgga cgtgccagag gttgctgggg cccatgtggc cggtctcggt gatgtccgcg
     9001 tccgctccgg ccagcgcccc gatcgcgccg tcgccggtgc aatcggcgaa ccagcgggcg
     9061 gaaaagcggg tgcgccggcc gctgcgaata tcctgggcca ccaccgcgcg cagaacaccg
     9121 cccacggttt cgacgccatt gacgcgctgt tcgaggaaca gggcgaggtt gcgctcgctg
     9181 cggaccaggg ccaggcggcg gtcgtcttcg taaatctcgg cggtgttatt cgtgcccacc
     9241 tgcgcccgca ccttgggctc cagctccgcc acaatatcac cgatgcgcgg atagggcggc
     9301 aggttaatgc cgccacccag ccaaacccgc acttccgaac tgttgttgcc gcccagcacc
     9361 gggcggtcct gcaccagcgc cacgctcagg cccaggcgcg cggcggacaa cgccgcgcag
     9421 gtgccggcga tgccgccgcc gacaacgacc aggtcgtagc gcccgccatt ttcgggcgcc
     9481 gcgggcaaac ccagcgcctg gcggcgaaag gcggtgaggg cggccagctc attgggcggc
     9541 gtgaaatcgg gatcgcggca gaacagaagg gcgtcgcagc ggccgccgaa gcccgtcagg
     9601 tcgtgcaaag caacccgggc ctcgcgcccg accttgacca tgccgccgtc ctgccagtgc
     9661 caggcggcgc cttcggtgcc aaaggtggtg gcgagcggct gaccgtcaat gagcacctgg
     9721 aatcgcccgg gcgcgccggg cgccttccag ggcgcaaccc aatcgcgggt gcgcacccag
     9781 acgcggtagg cgccggcggc gggaaagcgc gcgggggtcg aggcatcgcg aaccggctct
     9841 cccagcccgt gcgccagcag atagggagac cccatcaaat ccataaactg cggatccagc
     9901 acccagccgc ccgggtcggc aaattgctcc gcttcgagca ggacgaccgc cgctttccgg
     9961 ggcggccggg cggcgggaag cgttgcgggg cagaacaggc tcagggcgaa actggcggcg
    10021 aggatgagcc gcagtcggcg ggcgtttatg cgggtgtaga tcataggatt aatcaggttg
    10081 gccccatatc cggggagctc ggtaatcggc gggcttgggc tggttgcccg gcgcaaccgc
    10141 ttgccgggcg aaggcgtcgt agcgggcgcg gagttctttc accttttccg gatgctcggg
    10201 ggcgaggttg tctttctcgg aaacatcccg caccaggttg aacaattcga cttgcgacgg
    10261 cgggcgggtg cgggcggcgc gcttgccttt tttgggggcc gggccgggcg attccccgtc
    10321 gccgacgccc gccgcgccat tgaggaccag cttccaatcg ccgacgcgga tggccccgcc
    10381 gcgcggcgcc gcgttgatca ggattgcgtc atgcggcgaa ggctggcccc gcgtcagcac
    10441 ggccaacagg tcgtgcccgt ccagccgccg cgacggttcc agcggcgcgc ccgccagctt
    10501 gagcagcgtg ggataccaat ccaccatgtg cagcggctgc gggattgacg cgcccgccgg
    10561 gatgcgcccc ggccaggcgg cgcaggcgca gacgcgggcg ccgccctcat acaccgttcc
    10621 tttgccggcg cggagcgggc cgttgtggct gacgcggccg gggttggggc cgccgttgtc
    10681 gctggcgaag aagatgagcg tgttggtgcg gaagccgcgg gcatcgagcg tggccagaat
    10741 ctggccgatg gcctcatcca tcgccgccac catgccggca taagcgcggc gcggctgggg
    10801 gagatgggca taaggcacgg tgtatttttc cggcacttgg tgcggcgcat gcaccgcgtt
    10861 gaacggaaca tagaggaaaa acggtttatc ggcggcttgc tcgcgcagga gacgttgggc
    10921 ttcccgggcg atgagatgcg tgctgtagcc ctcgtcgcgg cagacgcggt cgtcgcggtg
    10981 ccagtcaaaa ccgccgtcgc gttcgtgggt gaagtaatca atggcgccgc agtaatgtcc
    11041 gtattggtga tcgaagccgc gcgcggtggg caggcaggcg cgctcaaaat tgcccaggtg
    11101 ccacttgccg acgatggcgg tggtgtagcc ggcttcccgg agcgcctggg gcagcaaccg
    11161 ctccgtcaac ggcaggccgc gctggtccca gggccggatc acgcccacct gcatgccgta
    11221 gcgcatcggg taacgcccgg tcatcagcgc ggcgcgcgtc ggggagcaaa gcggctgcgc
    11281 gtaaaactgt tccagccgcg cgcccgccgc cgccagccgg tcgagattgg gcgtccggat
    11341 gtctttggcg ccgttgaatc ccacatcggc gtagccgagg tcatccgcca gaaaaatcag
    11401 catattgggc cggggcgcgg ccgccgccga gcccagccaa agaagagcgg cgagcagaac
    11461 aagacgaccc ggcaacagat aattcataag cgcgctgact aagtgtaaga gcttgcgccg
    11521 gaagcgcgag cttattccac ctccacccag cagcgccgcc ggggcccttg gttatcggcg
    11581 tccgggtttc gatagctgcg ggagaaaagg ttcttccaac cccaaaaccg ttcttcgggc
    11641 tcagggggcg gggatgaaaa catctatcgc ttcaaagccg ccgactgacc ctgggggcaa
    11701 cgcaagcagt cacctttcgc caacctccgc ccgggagggg gaatgtcccc gccagccgtg
    11761 accccatccc gcccaaaaac cacgacgcac ggcaacacct cgccgcccgt tccagctttg
    11821 ccgaacgttc gcgtcctctc cctcccgcct tgaaacgtcc ttttacatca tccgcgactc
    11881 gtccattcct ccattccggc tcaggcccgc gcccaaaccc gccccacccg ccccagccag
    11941 cctaacgctc ccattaggct gtgtacaacg ggggtac
//
LOCUS       DAOPQG010000107        11749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6305_length_11749_cov_13.342740, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000107 DAOPQG010000000
VERSION     DAOPQG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6305_length_11749_cov_13.342740"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKI07_11205"
     CDS             complement(<1..478)
                     /locus_tag="PKI07_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC51652.1"
                     /translation="MDEPTADGTNGTLLATEKLVKEYRQRRVVNGVSITVAAGEIVGL
                     LGPNGAGKTTTFNMIVGVIRPDDGAVRFAARDITRLPMHKRARLGLGYLTQEPSIFRK
                     LTVAQNILAILETCRLSRAERAMRLKYLLDELDLTPLAKSKAYTLSGGEKRRLEITR"
     gene            complement(516..2348)
                     /locus_tag="PKI07_11210"
     CDS             complement(516..2348)
                     /locus_tag="PKI07_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOC51653.1"
                     /translation="MMNGRIGIGSVLGLAALAAALAQPAGEERAQPVTRGETLKLAEY
                     HAAPHDLQMKSLLEGARAQRQPDGRIVVTQARCQTFTPTGQGELVVEAPECVYDPKQR
                     TIRSAGALHVRTADDRLAIAGEGFTWQQTNSTLLVSNRVHTRLRPELFRPPGGAPLPS
                     APAPAGPTLDISSDRLEYAQTPGQGIYEGNVRVSGTNLNATAGRLILRSPMSEHRLQS
                     LTAEEHVVVDYEQIHATGAWAFYSADTDRVQLKGRPTWRIEQREGSGDELIFERSNRV
                     FQANGHARLQMPRASLGGASFLSRAEAAPTDGPAATRQFLEVRCDRYELRTNVAVFYG
                     PVRVNDWNGGQAQGQMTCGLLTLTLGATNELQRLVAERDVVIVQADRRFTAARAEYTA
                     GDRLLKLTGQPAWQDGPRAGRGDWMQVDLAREELSVRGNAVMKLPAGEVGQAVLTGAE
                     AARPVETRPGADGSAEIHSREYRFTEQTALFEGGVRIEHPQMQWTCDRLSLTSPPELG
                     PGGRRMVAEPAVVFDLLSEPGNPVHGTGERAVYTRRLAAARTNDFIELHGTPAMMASS
                     NLVIRNELIVLDISGRKLATPGKFNFRGPLPAAMTNHFRPEKRR"
     gene            complement(2345..3160)
                     /gene="kdsA"
                     /locus_tag="PKI07_11215"
     CDS             complement(2345..3160)
                     /gene="kdsA"
                     /locus_tag="PKI07_11215"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOC51654.1"
                     /translation="MLSDAAIWKSLSARSRWFLIAGPCVIESEQLCLQVASALKRACD
                     RMGVFYVFKASYDKANRTAATSFRGPGLDAGLAVLAKVRARVGVPILTDIHTEAQAVP
                     AAQVADVLQIPAFLCRQTDLIAAAARTGRVVNLKKGQFLSPEEMGRVVDKAQRAGGRK
                     LLLTERGTTFGYHNLVADMRAIPILRRLGCPVIFDATHSVQLPGGGGDCSGGQREFAP
                     VLARCALAAGANGVFIETHPHPERALSDGPNMIPLAEMPALLQSLKRIHSAVR"
     gene            complement(3269..4186)
                     /locus_tag="PKI07_11220"
     CDS             complement(3269..4186)
                     /locus_tag="PKI07_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC51655.1"
                     /translation="MTAPATSPAPPAAAAERPGLTKKQLAAEHPTWCPGCGDFSVLAL
                     FFKLIERRKLWQEKIVTVSGIGCASRFPYFVQANGAHYIHGRALPFASGVSLSRPDLH
                     VFVFGGDGDLFSIGGNHFTHTARKNIRITCVALDNWLYSMTKKQTSPTSPLGFKSKTD
                     LGGAIDYPINPARQAIAAGATFVARTVSSNPNHLLQMLEAAQDHNGFSFIQCLSECVE
                     FAPGAFDAANPRKGGVFNTVPADHDPTNEAAAYQLANEPFPGYFGILYQVNRPTKNAQ
                     EAALNQQASARFAGLKDWQILQKTFDRMK"
     gene            complement(4190..6037)
                     /locus_tag="PKI07_11225"
     CDS             complement(4190..6037)
                     /locus_tag="PKI07_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOC51656.1"
                     /translation="MSEPQRAAPAMSREARVSRISNAVIRIAGNSQDGIQTMGGLLAR
                     LAGRSAQEVMTFMTIPSTISGGPSIFQVHMGSGEVLSAGDEADVLLAFYQHSYEGHIG
                     ALKPGGIVLYDSDHVTPNAAWLPRYHHVGIPISSRTIEAFGGTKADKGKNLFALGVIA
                     RIFDLNETKLEALMSERFGARDERVLQNALAAFRAGYHHSTGNVLETFQFVESPDQPR
                     RQVVMNGNEALAYGLIAAGVRFGTAYPITPWSDVMEILRRELPNYGGTFVQCEDELAA
                     ISMANGASYAGRVAVTGSSGPGLSLKMESLGWAVMAEVPLVVINIQRGGPSTGLPTQV
                     EQSDLNLACFGSHGDAPRVVLAPADVEDCFYTAIEAVNLARQYNVPVLVLSDQAIASR
                     IEAFPEPDLERLCQDISPDLTPVADYKPYDLAAIPGATRRVVPGTPILSGRYPVASGL
                     EHDEYGHPSGAPQLHRAMTARRRRKLQALAATLPTPQVLGPPEGALLLVGWGSTRGPI
                     REATERARAAGESVSALHLRHLHPLPPGLEDIFAGFKAIRVVEMNDEGLYGYGQLAGL
                     LRARYCLPQIRGINKTDGLTWKVREILERARADRDAGLRPEPATAERHA"
     gene            complement(6117..7055)
                     /locus_tag="PKI07_11230"
     CDS             complement(6117..7055)
                     /locus_tag="PKI07_11230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOC51657.1"
                     /translation="MSDRSEVLTVSISQPSGRLDIYLRQKFPAVSRGAIQRLIEQGHI
                     RVNGRTVKPTHAPRAGEQIEVHWPAARPAAAQPEAMPLAVLFEDETLLVLNKPPGLVV
                     HPAAGHEEHTLVNALLHHCAGQLSGIGGIARPGIVHRLDKETSGCLVVAKNDETHLAL
                     SAQFASRKVEKIYQAILCGELPRDRGEIRAAIARHPSHRKRMAVDDDLGRDARTSYRV
                     RERLRGATLVEAVLHTGRTHQVRVHFQFLGFPLVGDPVYGSRPNLRLADLTGYTAPRQ
                     MLHASQLAFIHPRTARRLSFTAPLPEDFTEALAALR"
     gene            complement(7086..7625)
                     /gene="lspA"
                     /locus_tag="PKI07_11235"
     CDS             complement(7086..7625)
                     /gene="lspA"
                     /locus_tag="PKI07_11235"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOC51658.1"
                     /translation="MSLLSRSLGAPNRRIALVALLVVGLDQLTKQLVLRFLGYAQEKV
                     VVPGFFKFVHWGNTGAAWSLFKGNNELLAGVAIAALLALFLSRHLFDYRKPRSQVAFG
                     LIFGGIVGNLIDRLQVGHVIDFIYFYAERGGRELGFPAFNVADSAICTGVGLVFLITW
                     LNDRQAKRATAAPAKSAAE"
     gene            7946..9832
                     /locus_tag="PKI07_11240"
     CDS             7946..9832
                     /locus_tag="PKI07_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51659.1"
                     /translation="MPNEREVTLFTLTLEGETISLLPLRNWSQLDQYKWRARGKVPGT
                     PVGLEITHDYVKVASEVVSTQDPEGPAKLQKLLNEWLAAAAWGTPPPPPPEASPPAAA
                     PAASARAEDLAPHFRVEVNKLGQVLISCYQNKELLETIGLNLPGLTNLISQGLMHKPR
                     SLQVGALHDWIELDGEFFSFGKGQNDAARLEATLNARYRSTAALGRGREVVVFANAAS
                     PTGFDILFAAKVGGVIDHRRRPLNEQSLGLLQDYDRCGLLPKDMVIKLSPPNFIFKRK
                     TPDGGERYLDEGPESLVTVTGPRGEITMVDLSRPVNYSRLTAPELTAVFNHPAVNQHS
                     QASIERGAPSLKPELEPMAASPAAPAAPPPPPAPVAAPSSPVESQPAPVPGPQRGAAP
                     APAKPADARPASEPAPAAAPAAAPPPNLWLQEILRHTPIRRDWFNWLIYRRMAEFFGN
                     SNEGYFGPIPCWACSLNEAEDICDPDFRGIFLTQKGGLGYFNQGRIIRFCNDVVFIGT
                     LETAIEGIRVPLVAVGADSQCRLVFIVREGYRDQFGIPGPSLAGELELLREQGALLMS
                     IKEVLHSPEPIEVAWTVPSQQDDPANPQAMEYLRPGAEAEAEGREAPPADADDLMPPP
                     PGAD"
     gene            9841..11622
                     /locus_tag="PKI07_11245"
     CDS             9841..11622
                     /locus_tag="PKI07_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC51660.1"
                     /translation="MRASLLSRLALPGILLALAGCGRSDSASAGARHPLPPSPLVAQG
                     EPGRRGGRLVLPASASPQTFNPLFVFDSASDSIVRLLFASLVQFDWHAQEPGPGLAES
                     WSVAPDQKTWTFKLRPGVRWSDGQPLRAEDVVFTWNELILNRTFPATAFDLFRGGDPA
                     FAVTPMDDLTVRVVTREVYAPFLEFFGGVPILPKHILAATVQTKVFPAAYGLSSRPGR
                     IVGCGPYRLKEFRRGQFTLLERNPEYWVTDQQGTRLPYFDEIKFAVHTAAGSEATLLR
                     DGKSDVCDLIRPSSYAAFRHAAASGRFRLVELGAGAERDFLWFNQNTGTNAAGQPIVN
                     PIRLKWFRNRKFRQAVSCAIDRERLVREVYDGHARPACSFISSENHKWNNPDVPCYAY
                     DPDRARALLAEIGMLDRNGDGLREDADGHPLEIRFYSNTGNPLREQAALRIQEDLKQL
                     GLMLRYQPMDFRALVERINVTFDYECALMGLGGGGSDPASQMNVLRSSEELHQWFPFQ
                     KSPATDWEARIDTLAEAQMRTLDFAQRKKCFDEVQAILAEELPMIYIVTPFVAGAIRP
                     SVGNLRPAVLSPYPLTWNLPELYFKPQ"
BASE COUNT         1967 a         3894 c         3902 g         1986 t
ORIGIN      
        1 cgcgggtgat ttccagccgc cgcttttcgc cgccgctcag cgtgtaggcc ttgcttttgg
       61 ccagcggcgt caggtccagc tcgtccagca gatatttcaa tcgcatcgcc cgctccgccc
      121 ggctcaaccg gcaggtctcc aggatggcca ggatgttctg cgcgacggtc agcttgcgga
      181 agatggaggg ttcctgggtc agatacccca ggcccaggcg ggcgcgcttg tgcatcggca
      241 ggcgggtgat gtcgcgcgcc gcaaaccgga cggccccgtc gtccggccgg atgacgccga
      301 caatcatatt aaacgtggtg gtcttgccgg cgccgttggg accgagcagg ccgacaatct
      361 ccccggccgc gaccgtgatg gaaacgccgt tgaccacgcg gcgctggcga tattccttca
      421 ccagcttctc cgtggccagc aacgttccgt tcgtcccgtc cgccgtcggc tcgtccattc
      481 gaatgatttt cggcgtgcgc atggcaggct tcgcgtcaac ggcgtttctc cgggcgaaag
      541 tgatttgtca tcgccgcggg caacgggcct cggaagttaa acttccccgg cgtcgccagt
      601 ttgcggccgg aaatatccag cacgatcagc tcgtttcgaa tcacgaggtt ggaggacgcc
      661 atcatcgccg gagtcccgtg caactcgatg aagtcgttcg tccgggcggc ggccaggcgg
      721 cgggtgtaaa ccgccctctc gccggtgcca tgaaccgggt tgcccggttc gctcagcagg
      781 tcaaagacca ccgccggctc ggcgaccatc cggcgtccgc ccggccccag ctccggcggc
      841 gaggtcaggc tcagccggtc gcaagtccac tgcatttgcg ggtgctcgat gcgcacgccc
      901 ccttcgaaga gggccgtctg ctccgtgaac cggtattccc gcgaatgaat ctccgcgctc
      961 ccgtccgcgc cgggccgggt ttcgaccggt cgggccgcct ccgcgccggt caagacggct
     1021 tgccccactt cgcccgccgg caatttcatg acggcgtttc cccgcacgga cagctcctcg
     1081 cgcgccaggt ccacctgcat ccaatcgccc cggcccgcgc ggggaccgtc ctgccaggcc
     1141 ggttggccgg tcagcttcag gaggcggtcg cccgcggtgt actccgcccg ggccgcggtg
     1201 aaccgccggt ccgcctggac aatgacgaca tcccgctccg ccaccagccg ctgtaattcg
     1261 ttggtggcgc ccagggtaag cgtcagcaat ccgcaggtca tttgcccctg cgcctggccg
     1321 ccgttccagt cgttgacgcg caccggcccg taaaaaaccg ccacgttggt ccgcagctca
     1381 tagcgatcgc accgaacttc caggaattgc cgggtcgccg ccggcccatc cgtcggcgcc
     1441 gcttcggccc gcgaaaggaa gctggccccg cccaggctcg cacggggcat ctggagccgg
     1501 gcatggccgt tggcctggaa aacccggttg ctgcgttcaa atatcagttc gtcgccgctc
     1561 ccttcccgtt gctcgatccg ccaggtgggc cggcccttca actgaacccg gtctgtgtcg
     1621 gccgaataaa acgcccaggc acccgtggcg tgaatctgct cgtaatccac aacgacgtgt
     1681 tcctccgcgg tgaggctttg caggcgatgc tcgctcatgg gcgaccgcag gatcaaccgg
     1741 cccgcggtgg cgttcaggtt cgtgcccgag acgcgcacgt tcccctcgta aatcccctgg
     1801 cccggcgtct gcgcgtactc gagccggtcg gaggaaatgt ccagggtggg ccccgccggc
     1861 gcgggcgcgg agggcagggg agcgccgccg ggcggccgga acagctccgg ccgcagccgc
     1921 gtatggacgc gattggatac cagcagcgtc gagttggttt gctgccaggt gaatccttcc
     1981 ccggcgatgg cgagccggtc gtccgccgtc cgcacgtgga gcgctcccgc cgagcggatg
     2041 gtgcgctgct tcggatcata aacacattcg ggcgcctcga ccaccagctc gccctggccg
     2101 gtcggggtga aggtctggca ccgggcctgg gtcaccacga tgcgcccatc cggctggcgt
     2161 tgcgcccgag cgccttcgag gagcgatttc atctgcaaat catgcggcgc ggcatgatac
     2221 tccgccagct tgagcgtctc cccccgcgtc accggctgcg cgcgctcctc cccggccggc
     2281 tgcgccaggg cggcggccag cgcggccagc ccgagcacgg agcctatgcc gattcgcccg
     2341 ttcatcatcg gacggcggaa tgaatccgct tcaggctctg gagcaacgcg ggcatttccg
     2401 ccagcggaat catgttgggg ccgtcgctga gcgcccgctc cgggtgggga tgggtttcga
     2461 tgaacacccc gttggccccg gcggccaggg cgcaacgggc cagcaccggc gcaaactccc
     2521 gctgcccgcc cgagcaatcg cccccgccgc ccggaagctg aaccgaatgc gtcgcgtcaa
     2581 aaatcaccgg gcagcccagc cggcgcagga tcgggatcgc gcgcatgtcc gccacgagat
     2641 tatggtagcc aaacgtcgtg ccgcgttcgg tcagcagcag cttgcgtccg cccgcccgct
     2701 gcgccttatc caccacgcgc cccatttcct ccggggaaag aaattgcccc ttcttcaggt
     2761 tgaccacccg cccggtgcgc gccgccgcgg cgatcaaatc cgtctggcgg cagagaaagg
     2821 cggggatttg cagcacatcg gccacctgcg ccgccggcac ggcctgggct tcggtatgaa
     2881 tgtcggtcag gatcgggacg cccacgcggg cgcggacttt ggccagcacc gccagtcccg
     2941 cgtcgagccc ggggccgcga aaggaagtgg ccgcggtgcg attcgcttta tcgtagctgg
     3001 ccttgaagac gtaaaacacg cccatgcggt cgcaagcccg cttcagcgcc gacgccactt
     3061 gcaggcaaag ctgctcgctt tcaatcacac acggcccggc aatcaaaaac caccggctcc
     3121 gcgcgcttaa ggatttccaa atggctgcgt cgctaagcac atccagcttt taccagtgac
     3181 cgtggcgaat gtaaatgctc caccacggcc ccggtttggg caggggaatg aaatcgcccc
     3241 gaaagttgcc tgggccgccc gggcgggact acttcatccg atcaaaggtt ttctggagga
     3301 tttgccagtc cttgaggccg gcaaaccggg cgctggcttg ttggttgagg gcggcttcct
     3361 gtgcgttttt ggtcggccgg ttgacctggt agaggatgcc gaagtagccg gggaaaggct
     3421 cgttagcaag ctgataggcg gcggcttcgt tggtggggtc gtgatcggcg ggcacagtgt
     3481 tgaagacccc gcctttgcgt gggttggcgg catcgaatgc cccgggggca aactccacgc
     3541 attcgctcag gcattggatg aagctgaaac cgttgtggtc ctgcgcggct tccagcattt
     3601 gcagcaaatg attgggattg gaactgacag tgcgcgcgac gaaggtggcg cccgcggcga
     3661 tggcctgccg ggccgggttg atcgggtagt ctatcgcgcc ccccaggtcg gtcttgctct
     3721 tgaacccgag gggggaggtg ggcgaggtct gtttcttggt catgctatag agccagttgt
     3781 ccagcgccac acaggtgatg cggatatttt tgcgggcggt atgcgtgaaa tgattgccgc
     3841 cgatcgagaa caggtcgcca tctccgccga aaacaaacac gtggagatcg ggccgggaaa
     3901 gcgagacgcc gctggcgaat ggcagcgccc ggccgtgaat gtaatgcgcg ccgttggcct
     3961 ggacgaagta cgggaagcgg ctggcgcacc cgatgccgga gacggtgaca atcttttcct
     4021 gccacagctt gcgccgctcg atgagcttga aaaagagggc cagcacggaa aaatcgccgc
     4081 agcccggaca ccacgtggga tgttcggcgg cgagctgttt tttggtcagc cccgggcgtt
     4141 cggccgcggc ggcgggcggg gcgggagagg ttgcgggagc ggtcataaat caagcatggc
     4201 gttcggcggt ggcgggttcc gggcgcaggc cggcgtcgcg gtcggcgcgg gcgcgttcga
     4261 gtatctcgcg gactttccag gtcagcccgt cggttttgtt gatgccgcgg atttgaggca
     4321 ggcaatagcg cgcgcgcagc aggccggcca gctggccata gccatacagc ccctcgtcgt
     4381 tcatttccac gacgcggatg gctttgaagc cggcaaagat gtcttccagc cccggcggca
     4441 gggggtggag atgcctgagg tgcagggcgg aaacgctttc gccggccgcg cgggcgcgtt
     4501 cggtggcctc gcgaatcggg ccgcgggtcg aaccccagcc gaccagcaac agcgcgccct
     4561 cgggaggccc cagcacttgc ggggttggca gcgtggcggc cagcgcctgc agcttccggc
     4621 gccgccgggc ggtcatggcg cggtgcaact gcggcgcgcc ggacgggtgc ccgtattcgt
     4681 catgctccag cccgctggcg acgggatagc ggccgctcag gatcggcgtg ccgggcacca
     4741 cccggcgggt tgcgcccggg atggccgcca ggtcataggg cttgtaatcg gccacgggcg
     4801 tgaggtcggg cgagatgtcc tggcacaggc gctccagatc cggctcggga aaggcttcga
     4861 tgcgggaggc gatggcctgg tcgctcagca cgagcaccgg cacgttgtat tggcgggcga
     4921 ggttgacggc ttcaatggcg gtgtagaagc agtcttcgac atcagccggg gccagcacca
     4981 cgcgcggggc gtccccgtgg ctgccaaagc aggcgaggtt cagatccgac tgctcgactt
     5041 gcgtcggcag gccggtggac gggccgcccc gctggatgtt gatcaccacc agcggcacct
     5101 cggccatgac cgcccagccc agcgattcca tcttcagcga aagccccggc ccgctcgagc
     5161 cggtcacggc gacccgcccg gcatagctgg cgccgttggc catggaaatg gcggccagct
     5221 cgtcttcgca ttgcacaaag gtgccgccgt agttgggcag ctcgcggcgc aggatttcca
     5281 tcacgtccga ccacggcgtg atcgggtacg cggtgccgaa gcggacgccg gcggcgatta
     5341 atccgtaagc cagcgcttcg ttgccgttca tcaccacctg ccggcgcggc tggtccgggc
     5401 tttcgacaaa ctggaaggtt tccagcacgt tgccggtcga gtggtggtag ccggcgcgaa
     5461 acgcggccag cgcgttctgg agcacgcgct catcccgggc gccgaaccgc tcgctcatca
     5521 gggcttcgag cttcgtttca ttcaggtcaa aaatccgggc gatgacgccc agcgcgaaca
     5581 ggtttttccc cttgtcggcc ttggtgccgc cgaaagcctc gatcgtccgg ctggagatgg
     5641 ggattcccac gtggtgatac cgcggcagcc acgcggcatt gggcgtcacg tgatccgaat
     5701 cgtagagcac gattccgccc ggcttcagcg cgccgatgtg tccttcgtag gaatgctggt
     5761 agaaggccag cagcacgtcc gcctcatccc ccgcgctgag cacctccccg gagcccatgt
     5821 gcacctggaa gatggagggg cctcccgaga tggtcgaggg gatggtcata aaggtcatga
     5881 cctcctgggc gctgcggccg gccaggcgcg ccagcagccc gcccatcgtc tgaatgccgt
     5941 cctgggagtt gcccgcaatg cgaatgacgg cattggaaat tctcgatacc cgcgcctccc
     6001 ggctcatggc cggggcggct ctctgtggct cgctcatact ggataaaaca ctacccgccc
     6061 gccggtatct gtcaaagact aacgcgccgc gcggacccgc cggggcggcg tcctcctcag
     6121 cgcagcgccg ccagcgcctc ggtgaagtct tccggcagcg gggccgtgaa gctcaggcgc
     6181 cgggcggtgc gcggatggat gaaggcaagc tggctggcgt gcagcatctg gcgcggcgcg
     6241 gtgtagccgg tgaggtcggc caggcgcagg ttgggccggc tgccatagac cgggtcgccc
     6301 accagcggaa agccgaggaa ctggaaatgg acgcgcacct ggtgcgtgcg gccggtatgc
     6361 aaaaccgcct cgaccagcgt cgcgccgcgc aggcgctccc gcacccggta actggtgcgg
     6421 gcgtcgcgcc cgaggtcgtc gtccaccgcc atgcgtttgc ggtgcgacgg gtgccgggca
     6481 atggcggccc ggatttcgcc ccggtcgcgc ggcagttcgc cgcagaggat ggcctggtaa
     6541 atcttttcca ccttgcgcga ggcgaattgg gcggagagcg ccaggtgcgt ctcatcattc
     6601 ttggccacca ccaggcaccc gctggtttcc ttgtcgaggc ggtgcacgat gcccgggcgg
     6661 gcgattccgc caatgccgct caactggccc gcgcagtggt ggagcagggc gttgaccagg
     6721 gtgtgctctt cgtggcccgc cgccggatgc accaccagcc ccggcggttt gttgagcacc
     6781 agcagggttt cgtcctcaaa caacaccgcc agcggcatcg cctccggctg cgccgcggcg
     6841 ggccgggcgg cgggccaatg cacctcgatt tgctcgccgg cgcgcggggc gtgggtgggt
     6901 ttgacggtgc gcccgttgac gcggatatgg ccctgctcga tgagccgctg gatggcgccg
     6961 cgcgagacgg cggggaattt ctggcgcagg taaatgtcca gccgccccga gggctgcgag
     7021 atggatacgg tcagaacttc ggagcggtcg gacatggcgg aaagtccggg gcggcggggc
     7081 ggggcttact cggcggcgga ttttgcgggg gcggcggtgg cgcgcttggc ctggcgatcg
     7141 ttcagccagg tgatcaggaa gaccaggccc acgccggtgc agatggcgct atcggccacg
     7201 ttgaacgcgg ggaagccgag ctcgcgcccg ccccgctccg cgtaaaagta gatgaagtca
     7261 atcacatgcc ccacttgcag ccggtcaatg aggttgccca caatcccgcc gaagatgagc
     7321 ccgaaggcga cctggctccg cggtttgcgg tagtcgaaaa ggtggcggct cagaaacaac
     7381 gccagcagcg cggcgatggc caccccggcc agcagttcat tgttgccctt gaacaggctc
     7441 caggccgcgc cggtgttgcc ccaatgcacg aacttgaaga agccggggac gaccaccttt
     7501 tcctgcgcgt agcccagaaa gcggagcacc aactgcttgg ttaactggtc caggccaacc
     7561 accagcaagg cgaccagcgc aatgcgacgg ttcggggctc ccaacgacct actcaacaag
     7621 ctcattccag aaatgaccgc cccgactgta gcccgaaacc ggccccggaa acaagcgcaa
     7681 acaccgcgca catgacgctt gctaaaatcg ggcggaatcc gtaaattagg gagcggcccg
     7741 gttcacggct gttcggcgcc gggtttcatg cgatgaaacg gagccatcaa attgaatccg
     7801 agggcaggct cgatatggcg ctgcggagcg cccatccggc cacgctattt gccggaaggg
     7861 gaggggacgc atgagcgaat tgcgggcgca ggcatctccg gccgcttcgc cggaaaggat
     7921 aaccgtaggc cggacggcca tgtttttgcc caacgaacgg gaggtgacgc ttttcacgct
     7981 gaccttggaa ggcgagacca tcagtttgct gccgctgcgc aactggagcc aactggacca
     8041 gtataagtgg cgggcgcgcg gcaaggtgcc cggaacgccg gtggggttgg aaatcaccca
     8101 cgattacgta aaggtcgcca gtgaggtggt gtccacgcag gatcccgaag gccctgccaa
     8161 gctccagaaa ctcctgaatg aatggctggc ggccgccgcc tggggaaccc cgccgccgcc
     8221 ccccccggaa gccagcccgc ctgccgccgc gcccgcggcg tccgcgcggg cggaggatct
     8281 ggccccgcat tttcgggtgg aagtcaacaa actcgggcag gtgctcatct cctgctacca
     8341 gaacaaagag ttgctggaga ccatcgggtt gaatcttccc ggcctcacca atcttatcag
     8401 ccagggattg atgcacaaac cccgctccct gcaggtcggc gcgctgcatg actggattga
     8461 attggacggc gagttcttca gcttcggcaa gggccaaaac gacgccgccc ggctggaagc
     8521 gacgctcaac gcgcgctacc gctccaccgc cgcgctgggc cggggccgcg aggtggtggt
     8581 gtttgccaat gcggcctccc cgacgggctt tgacatttta ttcgcggcca aggtgggcgg
     8641 ggtcattgac catcgccggc gtcccttgaa cgagcaatcg ctggggcttt tgcaggacta
     8701 cgaccgttgc ggcctgctgc ccaaagacat ggtcatcaag ctgtcgccgc cgaacttcat
     8761 cttcaaacgc aagacccccg atggcggcga gcgctacctc gatgaaggac ccgagagcct
     8821 ggtcaccgtg accggcccgc gcggggagat aaccatggtg gacttgagcc gcccggtgaa
     8881 ctattcgcgg ctcacggcgc cggagttgac ggcggtattt aatcacccgg cggtcaatca
     8941 acacagccag gcttcgattg agcgcggcgc cccaagcctg aagccggaac tggaacccat
     9001 ggcggcttcc ccggccgccc ccgccgctcc gccgccgccg cccgcgccgg tcgcggcgcc
     9061 ctcgagcccg gttgaaagcc agccagcccc ggttccaggc ccgcaacgag gtgccgcgcc
     9121 cgcgcccgcc aaacccgccg acgcccggcc cgcttccgag cccgcccctg ccgcggctcc
     9181 ggcggccgcg ccgccgccca atttgtggtt gcaggaaatt ctccggcaca cccccatccg
     9241 gcgcgactgg ttcaactggc tgatctatcg ccggatggcg gaatttttcg gcaactccaa
     9301 tgaaggctat ttcggcccga tcccctgctg ggcctgcagc ctgaacgagg ctgaggatat
     9361 ctgcgacccg gacttccggg gtatctttct aacccagaaa ggcgggctgg gttatttcaa
     9421 ccagggacga attatccgtt tttgcaacga cgtggttttc atcggaaccc tggagaccgc
     9481 catcgaggga atccgggtgc ccctggtggc ggtgggagcc gattcgcaat gccgcctggt
     9541 cttcatcgtc agggaaggct accgcgacca atttggcatt cccggcccca gcctggccgg
     9601 ggagctggaa ctcctcaggg agcagggcgc cctgctgatg agcatcaagg aagtcctgca
     9661 cagccccgaa ccgatcgagg tcgcctggac cgtcccgagc cagcaggacg accccgccaa
     9721 tccgcaggcg atggagtact tgcgccccgg cgcggaagcc gaggccgagg gccgggaagc
     9781 gccgccggcc gatgcggacg atttaatgcc gccgccgccg ggagcggatt gaaaaaccgg
     9841 atgagggcat cgttgctttc ccgccttgcg ctgccgggca tcctgctggc cctggccggc
     9901 tgcggccgct ccgacagtgc ctccgccggc gcgcggcatc cgctgccccc ctcgccgctc
     9961 gtagcccagg gggagccggg ccgccgcggc gggcggctgg tcctcccggc cagcgccagc
    10021 ccgcagacct tcaaccccct gttcgtgttc gacagcgcat cagacagcat cgtgcgcctg
    10081 ctctttgctt cgctggtgca gttcgattgg catgcgcagg aacccggccc cggcctggcg
    10141 gaatcgtggt cggtggcccc ggatcaaaag acgtggacct tcaaactgcg ccccggcgtg
    10201 cgctggagcg acgggcagcc gctgcgggcc gaggacgtgg tttttacctg gaacgagctt
    10261 atcctcaacc ggacattccc cgcgaccgcg ttcgacctgt tccgcggggg cgaccctgcg
    10321 tttgcggtga ccccgatgga cgacctcacg gtgcgggtgg tgacgcggga ggtctatgcc
    10381 cccttcctgg agttctttgg cggcgtgccg atcctgccca aacacatatt ggcagcaacc
    10441 gtccagacga aagtctttcc cgccgcctat gggttgagtt cccgccccgg ccggatcgtt
    10501 ggctgcggcc cttatcggct caaggaattc cggcggggac agttcaccct gctggaacgc
    10561 aacccggagt actgggtgac cgaccagcag ggcacgcgcc tgccttattt tgatgaaatc
    10621 aagttcgccg tgcacacggc ggccgggagt gaggccaccc tgctccggga cgggaaaagc
    10681 gacgtgtgcg atctcattcg tccgtcgagc tatgcggcat ttcggcacgc ggccgccagc
    10741 ggacggttcc ggctcgtcga actcggcgcc ggcgcggaac gcgatttcct ctggttcaac
    10801 cagaataccg gcaccaacgc cgcgggccag cccatcgtca accccatccg gttgaaatgg
    10861 tttcgcaacc ggaagttccg ccaggcggtg tcgtgcgcga ttgaccggga acggctggtg
    10921 cgcgaggtct atgacgggca cgcccggccg gcgtgctcct ttatctcctc cgagaatcac
    10981 aaatggaaca acccggatgt cccctgctac gcgtatgacc cggaccgcgc ccgcgccttg
    11041 ctggccgaaa ttggcatgtt ggaccgcaat ggcgacgggc tgcgggagga tgccgatggc
    11101 caccccctgg agattcggtt ctactccaac accggcaatc cactccggga acaggccgcg
    11161 ctccggattc aggaagacct caaacaactg ggcctcatgc tccgctacca gcccatggac
    11221 tttcgggcgc tggtggagcg catcaacgtc acctttgact acgagtgcgc tctcatgggg
    11281 ctggggggcg gcggcagcga tccagcttcc cagatgaatg tgctgcgttc cagcgaagaa
    11341 ctgcaccagt ggttcccgtt ccagaaaagc cccgcgaccg actgggaggc ccgcattgac
    11401 acgctggcgg aggcccagat gcgaaccctg gacttcgcgc agcgcaagaa atgcttcgac
    11461 gaggtgcagg ccattctggc cgaagagctg ccaatgatct atatcgtgac cccttttgtc
    11521 gccggggcca tccgtccctc cgtgggcaac ctccgtccag cggtgctaag cccctatccc
    11581 ctgacctgga atctgcccga gctgtatttc aagccacagt aatgcgggcg ggcgcgtccc
    11641 gggccttgaa gcggggccgc ataacggttt agccccggcc gctgctccgc cggatcaaag
    11701 gcggggcttg atagagacaa ccctggcccc cggaaaaggc cgcgcaaaa
//
LOCUS       DAOPQG010000108        11654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6370_length_11654_cov_15.749806, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000108 DAOPQG010000000
VERSION     DAOPQG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11654
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6370_length_11654_cov_15.749806"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="PKI07_11250"
     CDS             <1..1428
                     /locus_tag="PKI07_11250"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOC51661.1"
                     /translation="RGSNKLKLSSAVANSSLRVGGGAVWHCLRARVCDFAPVHFLPLI
                     EAKREGQELAAEQIQEFIRAYTTGQIPSYQVAAFLMAVYFRGLTTAETRALTLAMRDS
                     GEVLQFPPDPRPLVDKHSTGGIGDKVSLPLAPLLACLGFRVPMISGRGLGITGGTLDK
                     LDSIPGFQSLPPAGRIVELVQRVGCVIGGQTERMVPADKELYALRDATATVPSIPLIV
                     ASILSKKLAEGLGALVLDVKFGAAAFMPTREKARALARALVTLSAECGVNTRALLTNM
                     ETPLGRAAGNWLEVKESVLCLDGRGPGDLRELVLACAAHLLVQTGKAATLAAAHAQAA
                     ACLDSGQPRRKWDEMIAAQGADLAAFAGKLARDHTAPVVAALKAPAAGFVARCDARII
                     GEVVRDLGGGRFTKEAGINYDVGVDALAKIGEAVPAGGVLARVHAADAAQADAACARL
                     KAAFEVAAQPVAAPPLIVETVEPAP"
     gene            complement(1440..2333)
                     /locus_tag="PKI07_11255"
     CDS             complement(1440..2333)
                     /locus_tag="PKI07_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC51662.1"
                     /translation="MTAPTKTALPALSRRAMMRCSVQATASLALATHCAPLLAAPRRR
                     RFKIGACDWSLGKQADPGAFALARQIGLDGVQVSLGTASDDMRLRQPGAQRQYRDAAR
                     QAGLEIASLAIGELNHVPYKSDPRAVPWVRDSIDAARALGVRVILLAFFHRGDLRDDP
                     AGVEEVVRRLKAVAPRAEKAGVTLGIESWLSAGQHLDLLQRVGSPAVRVYYDVCNAND
                     RGYDLYEEIRTLGPRICELHAKENGALLGQGKVDFHKVRAALDDIGYRGWIQIEGALP
                     PGQPLLESYQANCRFLRGVLA"
     gene            complement(2364..3074)
                     /gene="pyrF"
                     /locus_tag="PKI07_11260"
     CDS             complement(2364..3074)
                     /gene="pyrF"
                     /locus_tag="PKI07_11260"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOC51663.1"
                     /translation="MRNPILAALDVPTADEALRLVRQLAPVCGGFKVGSELFTAAGPG
                     IVRQIRGQNAAVFLDLKFHDIPHTVARAVAAATRLGVQMLTVHAGGGRSMLQAAEQAA
                     RETAAQMGQPPPLVLGVTVLTSLDDEALGQIGCEPHAGRQVLRLAQLAAQAGLRGLVC
                     SPHEITELRQSLPSALQLVIPGIRPGTVAGDDQKRTLTPRAALARGANWLVIGRPICA
                     APNPRAAAEQILNDLASP"
     gene            3161..4456
                     /gene="hisD"
                     /locus_tag="PKI07_11265"
     CDS             3161..4456
                     /gene="hisD"
                     /locus_tag="PKI07_11265"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOC51664.1"
                     /translation="MKVIRHTDASFRRRLRALAAASTLFDPAIEQRTRAILDDVRARG
                     DAALLELTERFDGARLRADQLPVTRAELMAAALKADAALRAAVAEADKNIAAFARRSR
                     RRNWEMRNSHGARVGEKFDPFQRVGVYIPGGTAPLVSTALMTVTLARVAGCPEIVVCT
                     PCGRDGSLNPAVLFAARAAGATEIYRVGGAQAIAALACGTATIRRVQKIFGPGNAYVV
                     AAKRLVFGRVAIDLLPGPSELLVLADDTANPKWVAADMLAQAEHGTGHERVWLATTSP
                     RLLKAVEREIKQQCARLPRRDLTRQVLARNGWLIQVRTLDEAVALANHLAPEHCEVML
                     RNPRRVAERLTTAGAVFLGPWTPTVLGDYVAGPNHTLPTGGAGASFAGLTVDQFQRRT
                     SLVEYPRTALRRALPAVRKFAELEGLSAHGRSAEIRLGA"
     gene            4473..5633
                     /gene="hisC"
                     /locus_tag="PKI07_11270"
     CDS             4473..5633
                     /gene="hisC"
                     /locus_tag="PKI07_11270"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOC51665.1"
                     /translation="MRSTSSRRPPALIPPRVRALRAYVPGEQPRTPGLIKLNTNENPY
                     PPAPGVLAATRAAVDGRLRLYPDPTAEELRRKLARLHRCRVENILVGNGSDELLALAV
                     RAFVEPAAGRRRASPSRVQFFHPSYSLYPVLAAIHGATANPVPLDADFALPTPARLRR
                     AGRWDFHAALTFVTTPHAPSGRGYRTAQLAALCRAQRGVIVLDEAYVDFAREQALALA
                     LRHRQVLVARTFSKAYSLCFQRVGYLVGHPELIAALHKIRDSYNVNGLGQVAAAATLD
                     HLPYYRAHFREIIATRQWLSRELAALGFQVFPSQTNFILVRPPGWAARDWLAKLRARK
                     VLVRWFSDPAVSDYLRVTIGTPSQAAALVKAVRALWPGVRAGSPRRRVTGRG"
     gene            complement(5617..6963)
                     /locus_tag="PKI07_11275"
     CDS             complement(5617..6963)
                     /locus_tag="PKI07_11275"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOC51666.1"
                     /translation="MTESWARRVLVLLCLLGACRALADLPPAPAAAAGSEAIPVTPPE
                     PVIFPLPPPVIWLAPHANTSLDFNVAVGSQFTCRWLHNGRPLAGAAGTRLDITDFKAA
                     QAGVYTLEVSNPYGSTKATTVLRLLNSPVVQVDGAIPAQATVTRTNTAEITLSSGFGS
                     DAAIYYTLDGSDPDFTATLYAGAFPLYNSATVRAMAYNFAHSQWAEAAPVRVEIWPAY
                     PLHVTTAGPGSVHLSPASVIATNYYPSNAVVQLTATATNGWGFIAWSGDITADAATTT
                     VLMDRPREVQAVFGQFPLLATTRGGGTIHVSPAAHSSSNIYAGRTLVTLTATPLKGWS
                     FIHWTGDSTATSAVTQVLLDQPRSVQAVFGAPLNLFTNGNGQVLVDPPTGPYLFGSTI
                     QLTPVPGEGYEFFGWANAASGKSNPLSFPVVTLPDITALFAVPKTSPPKVEVNLGP"
     gene            7198..8013
                     /locus_tag="PKI07_11280"
     CDS             7198..8013
                     /locus_tag="PKI07_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC51667.1"
                     /translation="MYLEYYGLTTPPFDITPNPRFLFYSAKHREAFNHLLYGIRERKG
                     FVQLTGEVGAGKTTLCRAMLEHLDDHYSTALILNPIMSAEELVKAIAQEYGLSVKGLD
                     RLETLAAINDFLLRQVERGKETLLIIDEAQDLTDDLLEQVRLLSNLETDDRKLLQIIL
                     FGQPELRDRLNNPRLRQLRQRITVRYHLRPLTRYEVSHYVQHRIHVSGGNGTPHFTRP
                     ALWRVHHYSQGIPRLVNAVCDKALLAGFVQQSDRIDYRMVGRAVRELEGNINA"
     gene            8010..8663
                     /locus_tag="PKI07_11285"
     CDS             8010..8663
                     /locus_tag="PKI07_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51668.1"
                     /translation="MSLINDALKRAKAAQQQTPPPPVNLPFRPVEPAPVYVRRGLGLA
                     MPVTLTIVACLALLLLWQWAQLRNAGDALEATARTAPIPEAAAAPLPASAAIPQPEAA
                     PLPEAPPPPVQATPETAPALPAAAEPAPAPAALAVAAAPEAQPAEAEPAVAPALPKPP
                     PLRLQAIIFDPRRPSAMINGKLVFLGDKVRDFRVARIDQESATLVGGGLTNVLTLLE"
     gene            complement(8745..9362)
                     /locus_tag="PKI07_11290"
     CDS             complement(8745..9362)
                     /locus_tag="PKI07_11290"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC51669.1"
                     /translation="MKTVLATLGAALLLAGGTLPPARAAEAASGASGKPLFNGKDLSG
                     WVPMHGGSFTVTNGLLRLVGGMGWLRTEKPYADFILEVEWRAREPGYDSGIFLRAGLE
                     GKPWPQDGWQVNLARNALGGLVKGNKTIVPAETPPLPVNQWVKFRLEARGRKITLEVD
                     GERAWEFHELDVPRGYLGLQAENKAFDFRNLRLQELPPEPQPAGQ"
     gene            complement(9544..>11654)
                     /locus_tag="PKI07_11295"
     CDS             complement(9544..>11654)
                     /locus_tag="PKI07_11295"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51670.1"
                     /translation="GANVVAFEVAGYNANSYYLLDQPSFLQAEVIVGGRVLAATGGKG
                     EPFTALLLPERVQKVQRYSFQRPFSEAYQLAPGYSRWRTEPIPKSAAAKVAVLPDRAL
                     LPRRVDYPDYAVRQPSWLVSQGQVKTGLKVEHPWKDRSLTAIGPKLGGYPEKELAIIP
                     SLELQTLTNATRQPVNRPWTPADSQVLPTHGYQIVDFGCNLTGFLGARINCRRPTRLF
                     FTFDEILVRDDVDFKRLGCVNIVSYELQPGSYELESIEPYTCRYLKLIVLEGECEVRS
                     LYLREYAGAGLARAQFAASDERLNRLFAAGVETFRQNALDLFMDCPSRERAGWLCDSF
                     FTARVAKDLCGDTRIEQTFLENYSLPPRFQHLPDGMLPMCYPADHNDGVFIPNWALWF
                     VVELEEYLARSGDRVLVDALRPRVLRLLDYFKKFRNDDGLLEKLESWVFVEWSAANSF
                     VQDVNYPSNMLYARALEAAGRIYGLTDLVDDAERIRETIRHQSFQGGFFVDNARRVNG
                     RLQITTNRTEVAQYFAFFFEIAKPETHSELWQRLVRDFGPQRKQTRAFPEVHPANAFI
                     GNVLRLELLSRYGLCQQLLDESLAYQLYMADRTGTLWENDGAYASCNHGFAAHGAVRV
                     LYRDVLGLREVDTVNRAVQLRFTDSRLDWCEGRYPTADGPVTLRWRKDGGRLLYQVSA
                     PAGYVIKAENRSQLEAVRQP"
BASE COUNT         1734 a         3751 c         4335 g         1834 t
ORIGIN      
        1 cgcggctcaa ataagttaaa gctttccagc gccgttgcca actccagttt gcgcgttggc
       61 ggcggcgcag tttggcattg ccttcgggcg cgggtttgcg actttgcacc ggtgcacttt
      121 ctgccattaa tcgaagccaa gcgcgagggc caggagctgg ccgcggagca aatccaggag
      181 ttcatccggg cctataccac cgggcaaatc ccgagctacc aggtggccgc ctttctcatg
      241 gcggtgtatt tccgcggctt gacgaccgcg gagacccggg cgctgacgct ggccatgcgc
      301 gactcggggg aggtgctgca atttccgccg gacccgcggc cgctggtgga caaacattcg
      361 accgggggga tcggagacaa ggtatcgctg ccgctggcgc cgctgctggc gtgcctgggg
      421 ttccgggtgc cgatgatttc ggggcgcggc cttggcatca ccggtggcac gctggacaag
      481 ctggattcga ttccgggttt tcagagcctg ccccccgccg ggcgcatcgt ggagctggtg
      541 cagcgcgtgg ggtgcgtcat cgggggccag accgagcgca tggtgccggc ggacaaggag
      601 ctgtatgcgc tgcgcgacgc cacggccacg gtgccgtcca tcccgctgat cgtggcgtcc
      661 atcctgtcca agaaactggc cgaggggctc ggcgcgctgg tgctggacgt gaagtttggc
      721 gcggcggcgt tcatgcccac gcgggaaaag gcgcgcgcgc tggcccgcgc gctggtgacg
      781 ctgagcgccg agtgcggcgt caacacccgg gcgctgctca cgaacatgga gacgccgctg
      841 ggccgcgccg ccggcaactg gctcgaagtc aaggaatccg tgctctgcct ggacggccgg
      901 gggcccgggg atttgcgcga gctggtgctc gcgtgcgccg cccacctgct cgtccagacc
      961 ggcaaggcgg cgaccctggc cgccgcgcac gcgcaggccg ccgcgtgcct ggattccggc
     1021 cagccgcggc ggaagtggga cgagatgatc gccgcgcagg gggcggatct cgcggccttt
     1081 gccgggaagc tggcccggga tcacaccgcg ccggtggtgg cggcgttgaa ggcgccggcg
     1141 gcgggttttg tcgcgcgctg cgacgcccgc atcatcggcg aagtggtgcg cgacctgggc
     1201 ggggggcgct ttacgaagga ggcgggcatc aactacgacg tgggggttga cgcgctggcc
     1261 aaaatcggcg aggccgttcc cgccgggggc gtgctggcgc gggttcacgc cgccgacgcg
     1321 gcgcaggcgg atgccgcatg cgcccggttg aaggccgctt tcgaggtggc cgcgcagccg
     1381 gtggccgcgc cgccgttgat cgtggagacg gtcgagccgg cgccgtagcg cccgcgcgct
     1441 caggccagga cgccgcggag gaaccggcag ttggcctggt agctttccag cagcggctgg
     1501 ccgggcggga gggcgccctc gatttgaatc cagccgcggt agccgatgtc gtccagggcg
     1561 gcgcggactt tgtggaagtc caccttgccc tggcccagca gcgcgccgtt ttccttggcg
     1621 tgcagctcgc agatgcgcgg gcccagcgtg cggatttcct cgtagaggtc gtagccccgg
     1681 tcgttggcgt tgcagacatc gtaatagacc cgcacggccg gggagcccac gcgctgcagg
     1741 aggtcgaggt gctgcccggc gctgagccag ctttcgatgc ccagcgtgac gccggccttt
     1801 tccgcccggg gcgcgacggc tttgaggcgg cggacgactt cctccacgcc cgccgggtcg
     1861 tcgcgcaagt cgccccggtg gaagaaggcc aggaggatga cgcgcacgcc cagggcccgg
     1921 gcggcgtcaa tgctgtcgcg cacccagggg acggcgcggg gatcgctttt gtagggcacg
     1981 tgattcaact cgccgatggc cagcgaggcg atttccaggc cggcctggcg ggcggcgtcc
     2041 cggtattgcc gctgcgcgcc gggctggcgg agccgcatat cgtcgctggc ggtgcccagg
     2101 ctgacctgca cgccgtccag gccgatctgc cgggcgagcg cgaaggcgcc cgggtcggcc
     2161 tgtttgccga gggaccagtc gcacgcgccg atcttgaagc gccgccggcg cggcgcggcc
     2221 aggagcggcg cgcagtgggt tgccagggcc aggctggcgg tggcctgcac cgagcagcgc
     2281 atcatggccc gccgggagag cgcggggagc gcggttttcg tgggggcggt catggggatg
     2341 ggggttgcgg gcggcagcgg gggttaaggg ctggcgaggt cgttgagaat ctgctcggcg
     2401 gcggcgcgcg ggttaggggc ggcgcagatc ggccggccaa tcaccagcca gttggcgccc
     2461 cgggcgagcg ccgcgcgcgg ggtgagggtg cgtttttgat cgtccccggc cacggtgccg
     2521 ggccggatgc cggggatgac cagttgcagg gcggagggga gggattggcg gagttcggtg
     2581 atttcgtgcg gggagcagac caggccgcgc aagccggcct gggcggccag ttgcgccagg
     2641 cgcaggacct ggcggccggc gtggggttcg cagccgattt ggccgagggc ttcgtcatca
     2701 aggctggtca gcaccgtgac gcccagcacc agcggagggg gctggcccat ttgggcggcg
     2761 gtttcccggg cggcctgttc ggcggcttgg agcatggacc ttccgccgcc ggcatgcacc
     2821 gtgagcattt gcacccccag ccgcgtggcg gcggccaccg cgcgcgcgac ggtatggggg
     2881 atgtcgtgga acttgaggtc gaggaagacc gcggcgttct ggccgcggat ttgccggacg
     2941 atgccggggc cggcggcggt aaacagctcg ctgccgactt tgaagccgcc gcagaccggg
     3001 gcgagctgcc gcaccagccg cagggcttcg tccgcggtgg gcacatccag cgccgcgagg
     3061 atgggattgc gcatgggcgc attcaaccag aacggcgccg gcgcaacaag gcgggactgc
     3121 ctttttgact tccgcccggg cttgctttag cctgcggccc atgaaagtta ttcgtcacac
     3181 cgacgccagc ttccgccggc ggttgcgcgc gctggccgcc gcttccaccc tgttcgatcc
     3241 ggccatcgag cagcggacgc gcgccatcct ggacgatgtg cgggcgcgcg gggacgcggc
     3301 gttgctggag ctgaccgagc gcttcgacgg cgcccggctg cgcgcggatc aactcccggt
     3361 gacgcgggcg gagttgatgg ccgccgcgct caaggcggac gcggcgctgc gcgcggcggt
     3421 ggccgaggcg gataaaaaca tcgcggcgtt cgcgcggcgg tcgcggcgcc ggaactggga
     3481 gatgcgcaac tcccacgggg cgcgggtggg cgagaagttt gatcccttcc agcgggtggg
     3541 cgtttatatt ccggggggca cggcgccgct ggtttcgacg gcgctgatga cggtgacgct
     3601 ggcccgggtg gcgggctgcc cggagatcgt cgtctgcacc ccctgcggcc gggacgggag
     3661 cctgaacccg gcggtgctgt ttgcggcgcg ggcggcgggc gcgacggaga tttaccgggt
     3721 gggcggcgcg caggccatcg cggcgctggc ctgcggcacg gccaccatcc ggcgcgtgca
     3781 aaagatattc ggcccgggca acgcctacgt ggtggcggcc aagcggctgg tgttcgggcg
     3841 ggtggccatt gacctgttgc ccgggccgag cgagttgctg gtcctggcgg acgacacggc
     3901 caacccgaaa tgggtcgcgg cggacatgct ggcgcaggcc gagcacggca ccggccacga
     3961 gcgggtctgg ctggcgacga cctccccgcg gctgctcaag gcggtggagc gggaaatcaa
     4021 gcaacagtgc gcccggctgc cgcgccggga tttaacccgc caggtgctgg cgcgcaacgg
     4081 ctggctcatc caggtccgga cgctggacga ggcggtggcg ctggcgaatc acctggcccc
     4141 ggagcattgt gaagtcatgc tgcgcaaccc gcggcgggtc gcggagcgcc tcaccaccgc
     4201 cggcgcggtc tttctggggc cctggacgcc cacggtgctg ggcgattacg tggcgggccc
     4261 gaaccacacc ctgcccaccg gcggcgcggg cgcgtccttt gccggcctga cggtggacca
     4321 gttccagcgg cgcaccagcc ttgtggaata cccgcggacg gcgctgcgcc gggcgctccc
     4381 ggcggtgcgg aagtttgccg aactggaggg cctgagcgcg catggccgca gcgcggaaat
     4441 ccggctgggc gcctgagccg ccgcgccgcg ctatgcgttc aacctcctcc cgccgtccgc
     4501 ccgcgctgat tcctccccgg gtgcgcgcgc tgcgggccta cgttcccggc gagcagcccc
     4561 gcaccccggg cctgatcaag ctcaacacca acgaaaaccc ttacccgccc gcgcccgggg
     4621 tgctggcggc gacgcgggcg gcggtggacg ggcggctgcg gctgtatccc gacccgaccg
     4681 ccgaggaact gcgccggaag ctggcgcggc tgcaccgctg ccgcgtcgag aatatcctgg
     4741 tcggcaacgg ttcggacgaa ctgctggcgc tggcggtgcg ggcgttcgtg gagccggcgg
     4801 cggggcggcg gcgcgcctcc ccgtcccgcg ttcagttttt tcatcccagc tattccctct
     4861 acccggtgct ggccgcgatt cacggcgcga cggccaaccc ggtgccgctc gacgccgatt
     4921 tcgcgctgcc cacgccggcc cgtctgcggc gcgcgggccg ctgggacttc cacgcggcgc
     4981 tgacgttcgt gaccacgccc cacgcgccga gcggccgggg ctaccgcacg gcgcaactgg
     5041 cggcgctttg ccgcgcgcag cgcggggtga tcgtgctgga cgaggcgtat gtggatttcg
     5101 cccgcgagca ggcgctggcg ctggcgctgc ggcatcggca ggtgctggtg gcgcggacgt
     5161 tttcgaaggc gtattcgctc tgcttccaac gggtgggcta tctggtgggg cacccggagc
     5221 tcatcgccgc cctgcataag attcgggaca gctacaacgt caacggcttg ggccaggtcg
     5281 ccgccgccgc gacgctggac catctgcctt actaccgcgc ccatttccgc gagattatcg
     5341 ccacgcgcca gtggctgagc cgggaactgg cggccctggg attccaggtc ttccccagcc
     5401 agacgaactt catcctcgtt cgcccgccgg ggtgggcggc ccgggactgg ctggcgaagc
     5461 tgcgcgcgcg caaggttctg gtgcggtggt ttagcgatcc ggcggtcagc gattatctgc
     5521 gcgtcaccat cggtacgccg tcgcaggcgg cggccctggt aaaagccgtc cgggcgcttt
     5581 ggccaggcgt gcgggcgggt tccccgcgcc ggcgggttac gggccgaggt tgacctcgac
     5641 tttgggcggg ctggtcttgg gtaccgcgaa gagggcggta atgtccggca aggtgacgac
     5701 cgggaagctc agcgggttgg atttgccgct ggcggcgttg gcccagccaa agaactcata
     5761 gccctcgccc ggcaccggcg tgagttggat ggtcgagcca aacaagtagg ggccggtcgg
     5821 cgggtccacc agcacctgcc cgttgccgtt ggtaaagagg ttcaagggag cgccaaaaac
     5881 cgcctgcacg gatcggggtt gatccagcag cacttgcgtc accgcgctgg tggcggtgct
     5941 gtccccggtc cagtgaatga acgaccagcc cttgagcggg gtggcggtga gcgtgacgag
     6001 cgtccggccc gcgtagatat tgctgctgct gtgggcggcg ggggaaacgt ggatggtgcc
     6061 gccgccccgg gtggtggcca gcagggggaa ctgcccgaat accgcctgca cctcgcgggg
     6121 gcgatccatc agcaccgtcg tggtggcggc gtcggcggtg atgtcgccgc tccaggcgat
     6181 gaatccccag ccgttggtgg ccgtggcggt gagttggacg accgcattgc tggggtagta
     6241 gttcgtggcg atgaccgacg ccggggagag atgaacgctg ccggggcccg cggtggtgac
     6301 gtggagcggg taggccggcc agatttcgac gcggacgggc gcggcttccg cccattggga
     6361 atgcgcgaag ttataggcca tggcgcgcac cgtggcgctg ttgtagaggg ggaacgcccc
     6421 cgcatacagc gtggcggtga aatccggatc actgccgtcg agggtgtagt agattgccgc
     6481 gtcggagcca aacccgctgc tcagcgtgat ttccgccgtg ttggtgcggg tcaccgtggc
     6541 ttgggccggg atggcgccgt ccacctggac gaccgggctg ttcaggagcc ggagcacggt
     6601 ggtggccttc gtgcttccat aggggttgga gacctccagg gtatagacgc ccgcctgggc
     6661 tgccttgaag tccgtgatgt ccagtcgggt tcccgcggcg ccggcgagcg gccggccgtt
     6721 atgcagccac cggcaggtaa actggctgcc caccgcaacg ttgaagtcca ggctggtgtt
     6781 cgcatgggga gcgagccaga tcaccggcgg cggcagcggg aaaatgacgg gctccggcgg
     6841 cgtcaccggg atggcctcgg aaccggcggc ggccgccggc gcgggcggca gatcggccag
     6901 cgcccggcag gcacccaata aacagagcag aaccagcacc cgccgcgccc atgattcggt
     6961 catattcatg acgggtgata ttgaattgcg gcgctcaaaa agcaatgaga aaatcccggg
     7021 cggcgaacgg ccgccgggcc ggggggaggg gcgggctggc tgaggaatgg acagaaccgc
     7081 ccgcttttga ggcacgcggc gggcgggcgg gggcgttggc ggcgcaaagc aggcgtccgg
     7141 accggcctta accgccgccg tccggccgca ggcatccttt ctgctacaca ggcaagcgtg
     7201 tacttggaat actatggcct gaccacgcca ccgttcgaca ttacgccgaa tccgcgcttc
     7261 ctgttttaca gcgccaagca ccgggaagcg ttcaaccacc tgctttatgg cattcgcgag
     7321 cgcaaggggt tcgtgcagtt gaccggcgag gtcggcgcgg gcaagaccac cctctgccgc
     7381 gcgatgctgg agcacctgga cgaccattac tccaccgccc tgattctcaa tccgatcatg
     7441 agcgccgagg aactggtcaa ggcgatcgcg caggaatatg gcctgtccgt caaggggctg
     7501 gaccggctgg aaaccctggc ggcgatcaac gacttcctgc tgcgccaggt cgagcgcggc
     7561 aaggaaacgc tgctgatcat tgacgaggcc caggacctga cggacgattt gctcgagcag
     7621 gtgcggctgc tctcgaacct ggagaccgac gaccgcaagc tgctgcagat tatcctcttt
     7681 gggcagccgg agctgcgcga ccggctgaac aacccgcggc tccgccagct ccgccagcgc
     7741 atcacggtgc gctaccatct gcggccgctg acccgctacg aagtcagcca ctacgtgcag
     7801 caccggattc atgtctcggg cggaaacggc acgcctcatt ttacgcgccc ggcgctctgg
     7861 cgggtgcatc attacagcca gggcattccc cggctggtca acgcggtctg cgacaaggcc
     7921 ctgctggcgg gctttgtcca gcagagcgac cggattgatt accgcatggt cggccgcgcg
     7981 gtgcgggaac tggaaggaaa tatcaacgca tgagcttaat caatgacgcc ctgaagcggg
     8041 ccaaagcggc gcaacaacag acccccccgc cgccggtcaa tctccctttt cgcccggttg
     8101 aacccgcccc ggtgtacgtc cggcgcggcc tgggcctggc aatgcccgtc acgctgacga
     8161 tcgtcgcctg cctggcgctg ctcctgctct ggcaatgggc gcaactccgc aatgccggcg
     8221 atgccctcga agccaccgcc cgcaccgcgc cgatcccgga agccgccgcc gcgccgctcc
     8281 cggcttccgc cgctatcccg cagcccgaag cggcgccgct gccggaggcg ccgccgccgc
     8341 ccgttcaagc aaccccggaa accgcgccgg ctctccccgc ggcggccgaa cccgcgcccg
     8401 cgccggcggc gttggcggtt gcggcggccc cggaagcgca acctgccgag gccgagccgg
     8461 ccgtggcccc cgctttgccc aaaccgccgc ccctgcggct gcaggccatt atcttcgatc
     8521 cccggcgtcc ctcggccatg attaacggca aactggtttt cctgggcgac aaggttcgcg
     8581 atttccgggt ggccaggata gaccaggaaa gcgctacgtt ggtgggcggc gggctgacca
     8641 acgtcctgac cctcttggaa taaccgcgct ccccgcgcgg cgcggggcgt tgcccggcgc
     8701 tgacccgggg cgggcgggcc ggcccccgac gccgccgccg ccggttattg cccggcgggc
     8761 tggggttccg gcggcagttc ctgaaggcgc aggttgcgga agtcgaaagc tttattctcg
     8821 gcctggaggc caaggtagcc gcgcgggaca tccagttcgt ggaactccca ggcccgttcg
     8881 ccatccacct ccagcgtgat tttccggccc cgcgcttcaa ggcggaactt gacccactga
     8941 ttcaccggca ggggaggcgt ctccgccggc acgatggttt tattgccttt caccagcccg
     9001 cccagcgcgt tgcgcgccag gttcacctgc cagccgtcct ggggccaggg tttgccctcg
     9061 agtccggcgc gcaggaaaat gccgctgtcg tatcccggct cccgggcccg ccattcaact
     9121 tccaaaatga agtcggcata gggcttctcc gtgcgcagcc atcccatgcc gccgacgagg
     9181 cggaggaggc cgttggtgac ggtgaaagac ccgccgtgca tcggcaccca gccggacagg
     9241 tctttgccgt tgaagagggg tttcccggac gcgccggacg cggcctcggc tgcccgggcc
     9301 ggcggcagcg tcccgcccgc cagcagcagg gcggcgccga gtgttgccag gaccgtcttc
     9361 atgtccgtgt attcaacggg gttgccatgc ggagggcagc ctatcgcgcg ccggggcggg
     9421 gtgaaaggcc aaaagcgacc ggggcaaccc ggcatttgcc cgcgccgccg cgccccggct
     9481 tccggagccg gctgcccgcc cgggccgcgc ccgcgcctgg cggaggaaag tttccgcacc
     9541 ggctcaaggc tggcgcacag cttccagttg gctgcggttt tccgccttga tgacgtagcc
     9601 ggcgggggcg ctgacctggt agaggagccg gccgccgtcc ttgcgccagc gcagcgtgac
     9661 ggggccgtcc gcggtcgggt agcggccttc gcaccagtcg aggcgcgagt cggtgaagcg
     9721 gagctggacg gcgcggttga cggtatccac ttcgcgcagg cccagcacgt cgcggtaaag
     9781 gacgcggacg gcgccgtggg cggcgaagcc gtggttgcaa ctggcgtagg cgccgtcgtt
     9841 ttcccacagg gtgccggtgc ggtcggccat gtagagctgg taggcgaggg attcatcgag
     9901 caactgctgg cacaggccgt agcgggacag cagttcgagg cgcaagacgt tgccgatgaa
     9961 ggcgttggcg gggtggactt cggggaaggc cctggtctgc ttgcgctggg ggccgaagtc
    10021 ccggaccagg cgctgccaca gctcggagtg cgtctcgggt ttggcgatct cgaagaagaa
    10081 ggcgaagtat tgggcgacct cggtgcgatt ggtggtaatc tgcaaccggc cgttcacgcg
    10141 gcgggcgttg tccacgaaga aaccgccctg gaaagactgg tggcggatgg tttcgcggat
    10201 gcgttcggcg tcgtccacga gatcggtgag gccgtagatt cggcccgccg cctcgagggc
    10261 ccgggcgtag agcatgttgg aggggtaatt gacatcctgc acgaacgagt tggcggcgga
    10321 ccactcgacg aaaacccagc tctcgagctt ctccagcagg ccgtcgtcgt tgcggaactt
    10381 cttaaaataa tccagcaggc gcagcacgcg ggggcggagg gcgtccacca gaacgcggtc
    10441 gccgctgcgg gccaggtatt cctccagctc gacgacgaac cagagcgccc agttggggat
    10501 gaacacgccg tcgttatggt cggcggggta gcacatgggc agcatgccgt cgggcagatg
    10561 ctggaagcgc ggcggcaggg agtagttttc gaggaaggtt tgctcgatgc gggtgtcgcc
    10621 gcaaagatct ttggcgacgc gggcggtgaa gaagctgtcg cagagccagc cggcgcgctc
    10681 gcgcgagggg cagtccatga agaggtcgag ggcgttctgg cggaaggtct cgacgccggc
    10741 ggcgaagagg cggttgagcc gttcgtcgct ggcggcgaac tgggcccgcg ccaggccggc
    10801 gccggcatat tcgcggagat acaggctccg gacctcgcac tcgccctcga gcacgatgag
    10861 cttgaggtag cggcaggtgt acggctcgat ggattccagc tcgtagctgc ccggctgcaa
    10921 ctcatagctc acgatgttca cgcagccgag gcgcttgaag tccacgtcgt cgcggacgag
    10981 aatttcatca aaggtgaaaa acaggcgggt ggggcggcgg cagttaatcc gcgcgccgag
    11041 aaagccggtg agattgcagc cgaagtccac gatctggtaa ccgtgggtgg ggagcacctg
    11101 ggagtcggcg ggcgtccagg gccggttgac gggctggcgg gtggcgttgg tgagggtttg
    11161 caattccagc gagggaatga tcgccagctc cttttccggg tagccgccga gcttcgggcc
    11221 gatggccgtg agggaacgat ccttccaggg gtgctccacc ttgagaccgg ttttgacctg
    11281 cccctgggat acgagccagg aaggctggcg aacggcgtag tcgggataat ccacgcggcg
    11341 gggcagcagc gcccggtcgg gcaggaccgc gactttggcc gcggcggact tgggaattgg
    11401 ctcggtgcgc cagcggctgt agccaggcgc gagctggtag gcctccgaga aggggcgctg
    11461 aaaactgtag cgctgcacct tttgcacccg ctccggcagc aaaagggcgg tgaacggctc
    11521 gcctttgccg cccgtggcgg ccagaacccg gccgcccacg atgacttcgg cctgcaggaa
    11581 ggagggctgg tccagcaggt aatagctgtt ggcgttgtag ccggccacct cgaaggcgac
    11641 gacgtttgcg cccg
//
LOCUS       DAOPQG010000109        11649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6375_length_11649_cov_17.109194, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000109 DAOPQG010000000
VERSION     DAOPQG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11649
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6375_length_11649_cov_17.109194"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(805..1713)
                     /locus_tag="PKI07_11300"
     CDS             complement(805..1713)
                     /locus_tag="PKI07_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOC51671.1"
                     /translation="MQIESLKVFCDLAETRNFTRTAKLNGVSQSAISQQLSSLERQFL
                     PWKAGPEQKKSLLERGNRKCQLTPEGRALYQYSKRILALYGDLQSRLQETRELISGTI
                     RVATIYSIGLHDLAPYLKQFLKDYPTVNVQVEYRRANQVYEDVASNVVDLGLVAFPAS
                     NKKLEIYPLREEELVLICHPQHPLAGKKNLPLAALHQQKFISFERDIPTRKALDKIFK
                     SHSIQVKHVMEFDNIETLKCAVEIDAGLAIVPQSTVVQEVKQGTLAQVTLEGGAFHRP
                     LAALYQKKKTLTPAIKQFLALLRGES"
     gene            complement(1747..2190)
                     /gene="cdd"
                     /locus_tag="PKI07_11305"
     CDS             complement(1747..2190)
                     /gene="cdd"
                     /locus_tag="PKI07_11305"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOC51672.1"
                     /translation="MAKLNKLALVRAAARARRGAVAPYSRFQVGAALLTRSGQVITGA
                     NVESASYGLTCCAERVALFNALTAGQRDFVAVAVVARTAGGPMPCGACRQLLAEYAPR
                     AAVWVADSRSLGAVRAFTVKELLPAAFVLTPKRNARRPGSARRGG"
     gene            complement(2174..3088)
                     /locus_tag="PKI07_11310"
     CDS             complement(2174..3088)
                     /locus_tag="PKI07_11310"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOC51673.1"
                     /translation="MKNSANQRGSRSRPRRNRPDDSVAPKPGKPSRQPDPQTAAARLR
                     QVSRLRPTLALVLGSGFQHVLSELQVDADIAYQRLPGFPATGVSGHAGRLVIGRLGGT
                     PVMVLSGRAHYYEGHAMAVVTFAVRALAAYGIRDLLLTNAAGGVNRSFRPGDFMVLTD
                     HVNFMGLNPLRGEPAPGRPRFVDLTCVYDPGWRRLLARAGRACGVKLRSGVYWAVSGP
                     SYETPAEIRAFARLGADAVGMSTVPEAIVARQCGLKVAGISCITNLAAGRGRPTLSHA
                     EVLETAERVQRVAARLLETFAQLYGQTQ"
     gene            3226..3302
                     /locus_tag="PKI07_11315"
     tRNA            3226..3302
                     /locus_tag="PKI07_11315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3260..3262,aa:Arg,seq:ccg)
     gene            3382..4140
                     /locus_tag="PKI07_11320"
     CDS             3382..4140
                     /locus_tag="PKI07_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOC51674.1"
                     /translation="MKFFTTISALLIAALGLAPGAVQGQAPAPAPELHQCVKPVPREG
                     QGYQRYVELNQRVRDSQGQARVIFVGDSITQGWEGNGKEVWAKYYAPRQALNLGIGSD
                     HTQHVLWRLDHGNLDGLHPKVAVVLIGVNNIPDTNNTPRQVLEGVTAVVRKLREKLPE
                     TKVLLLGIFPFREDFCDQRAKALQVNQALHKLDDGHWIYFRDIGHLFIQPDGRISPAM
                     MRDFLHLSPTGYRLWAEAIEPDLAALLGEKPITP"
     gene            4733..4981
                     /locus_tag="PKI07_11325"
                     /pseudo
     CDS             4733..4981
                     /locus_tag="PKI07_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019058470.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
     gene            5082..7784
                     /locus_tag="PKI07_11330"
     CDS             5082..7784
                     /locus_tag="PKI07_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51675.1"
                     /translation="MAFLRRCLLILAVLLAAGARLAAASAADRAFGAAVKAFQDSFYE
                     RAEAQLAEFSQKHPASPRRAEAILLQAQARLMLTNYAGAIELLSAHQGAAGPLADQYA
                     FWLAEAYSRKGDWRAASDAFARMSREFPASPRGLEAVIGEASARAALAQADPAEWPRL
                     IALLQATNGVFQTAARAAGDSELVAQGWLLLGEAQFATGDYRAAEATLQPLAGQLLKP
                     ELAWSWQYLLCRIQVADGRPEAALPGATNLMALAARVVPDNRRAESAAFQAGLFERLG
                     RFEDAIAAYQQNLTDGVSPERQRQALLKISELYLAHDRIPEATQSLEKFLAQYPEAAA
                     ADLALQTLGELRLRQYETARGAQPLPAPPALAPDATNNLRQARESFEALTKRFPQSPL
                     FGKAQLDLGECYWWEGQMPEAAAAFQAAVERLPRSLDQARACSRLADACFRQTNYAGA
                     IKYYQAVIEQYADLPEARTNLFARALDQTVRAGLAAGDLAAATNALQKALDWYPDRPD
                     TARALLFTGQEISRRGDPAAARQIFLEFTNRAPHTPLLAEVRLAVAGTYEQERNWPEA
                     IAQYDAWLAAFPNNPAQPRAEYARAWNSFQAGRETNALTSFTNFIARFPTNEFAPLAQ
                     WWVADYYYRAGDPMEAERNYKLLFLNTNWPPSELTYEAQLMAGRAAVARQGWGEARGY
                     FTSLYNNTNGPSLDLRLQALFELGLALMRTVDASETNRLANCEEATRAFGRLCDDYPT
                     NRLAVRAWTEKANCYLQWALARQQYDSLTNALNAYQQVVDSPLAEVPARSQAKVGQAI
                     VLSKWAEQKTGDERTALLKTALSNCLDVVYGSLLREGEQLDPFWTREAGIEAFGLAEA
                     MQAWSQAASVYQRLTNSVWPQLPASLEKRAWQARENLAREKNGH"
     gene            7863..8501
                     /locus_tag="PKI07_11335"
     CDS             7863..8501
                     /locus_tag="PKI07_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOC51676.1"
                     /translation="MTLSPAAGTPALGVVLLAAGRSARMGKPKLLLPWGDTSVLGHLI
                     RQWQSLGVAQLSVVCAAGDSALAAELDRLGFPAENRILNPATERGMFSSLQCAALWRG
                     WRAEVTRWAVALGDQPQVRLQTLRQVLEFSAAHPARICQPARRGRGRHPVLLPEAAFR
                     QLATAAVSTLRDFLAAHPEGVVFCEADDPGLDLDIDRPEDYEQVLRLGGLVP"
     gene            complement(8584..9819)
                     /locus_tag="PKI07_11340"
     CDS             complement(8584..9819)
                     /locus_tag="PKI07_11340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC51677.1"
                     /translation="MPFTRFGLSPAVLQGVRAMGYLEPTPIQLRAMPLILEGKDVIGS
                     AQTGTGKTAAFALPILSKLGTHGPGPRLVVLEPTRELAAQVETALHNFARFTNLRTAV
                     IFGGVGYGKQLEALRDGVDVLVATPGRLLDHLQRGTCRLDRVQFVVLDEADRMLDMGF
                     LPDVRRILDRCPRNRHTSLFSATIPPEIETLIQWAMRQPQTIEIGVRRSPKDTVKHVI
                     YPVSDFQKADLALELLRRVNYDSVIVFCRTKHGADRVAGLLKRNQHAVAVLHSNRTQR
                     EREQALRGFRDGRFEVLVATDIAARGLDIADVSHVINYDVPQHPEDYVHRIGRTGRME
                     ATGDAYTLMVAEDVRHVNAIEHFIGQKIPRVKLENFNYRYTTLFEQGKPGQPPAGGGR
                     VRGARVRGGYFFGPVRRRR"
     gene            complement(9859..11520)
                     /locus_tag="PKI07_11345"
     CDS             complement(9859..11520)
                     /locus_tag="PKI07_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51678.1"
                     /translation="MSILSDNEFDLNKLFLPAWAQEPSASKYANYEGETERFDRRRDR
                     DRRGPRPPRREGPAGPRREGDRPRGERRGPRRPEGGRDQAPGGPKADFRRGEPRERRA
                     PRPLPEIEASLRPEDKGVESLARQIKMTGRAYPLFEIAQMILQKPERHQVLFSVKKNP
                     QGQPIQPLFVCALDDTLWLSEDEAIAHVLKQHFATFYQAERTATEPPKGKYTFVAQCG
                     LSGRILGPPNHHDYQNQLRKLHSERFARMPFEEFKSRVRIVRDEEVVKKWVDDRSWKT
                     EYVCLNLPEPVRLTSREAVEQHFRENHKENIIQSVESHSLNGAAAHALRSPDLARLVR
                     SVCEDQRRFPLQLATVLSQQFAGHGLQFFKVNKTLTHVCIARPRYLDVAATPVSEGVK
                     RIVEYINATPKCTRKQLIEALAPSPPPVPTAEAGAAPAPPVESAEPTPEQTAVIADLH
                     WLIHEGHVIEFATGILETAKKPQPKPEPKPAAKPAPAEAAPIPAAETPPPPPAPAPEI
                     APAAASEAAIAPETAENEVPPAAPPENLPEVPPEMPPADPVGPLP"
BASE COUNT         1917 a         3764 c         3910 g         2058 t
ORIGIN      
        1 aacaccttga gtggaaacca tgacgaccaa tttgctgaac ttgacggaca caactagccc
       61 caatgccaaa actaataatt gcaacccgcg ttgcctttcc gatcccaacc atgcggaaga
      121 aacctggatt cccggacagt ccctgattac ccgcccgtcc ttgaaaaacc cctttccacc
      181 agtctcagat ttataactaa ctgtttttac ggaacttagt aacaattcca ccgttctccc
      241 acatcgtttc ggccttccca ggcatccgaa ttcaatatcc agataccctg aaattaccct
      301 gccatcaggc cgtgtacaag gggtgtacaa gggatgctca tcgggtgtac cagctaaaat
      361 cccatggacc cccggtgagc atcccttgta caccccttgt acacggcctg atggcagggg
      421 atgggcaggg tgcgtccggg gtaagccgaa gccggcatga ttcagatttt gctcggtttt
      481 ggccctctct atgcctaact cgttgatttt gcagacggaa ggcttggaat gcagcagcta
      541 taccgctgat ttcatcaaat aaagggttcg ccggggccac ggtttggccg gtcaggcccg
      601 cccagacctc gataaagcgg gggtgggtgg ttgaaatagc tggctgaatc gtttcggctg
      661 gaggcgtcag gggcgattcc cggcatcgga aggggtggcg tatccgttag gccacagctt
      721 ggttccctcc gggaaacggc cggaaccgaa tagttccctt cgggcaccac gggcgatgcg
      781 gaaccgaagg acgctaagcc agccttatga ttcgccgcgt agcagcgcca ggaactgctt
      841 gattgccggc gtcagggtct ttttcttctg atagagagcc gccagcggac gatggaaggc
      901 cccgccctcg agggtgacct gggccagagt gccttgtttg acttcctgta cgacggtgct
      961 ttgaggcacg atcgccagcc cggcatcaat ttccacggcg catttgagcg tctcgatgtt
     1021 gtcgaactcc atcacgtgct tcacctggat ggaatggctt ttgaagattt tgtcgagggc
     1081 tttgcgggtg ggaatatccc gttcgaagct gataaacttc tgttggtgga gggcggcgag
     1141 cgggagattc tttttcccgg ccaggggatg ttgcgggtgg caaatcagca ccagttcttc
     1201 ctcgcgcagc gggtagattt ccagtttttt gttgctggcg ggaaacgcga ccaggcccag
     1261 gtccaccacg ttgctggcca catcctcata cacctggttg gcgcgccggt attccacctg
     1321 cacattcacg gtggggtagt ctttgaggaa ctgcttcaga tacggcgcca gatcatgcag
     1381 gccaatgctg tagatggtgg cgacgcggat ggttcccgag atcaactccc gggtctcctg
     1441 cagccgggac tgcaggtcgc cgtagagggc gagaatccgc ttgctgtatt ggtagagcgc
     1501 ccggccttcg ggggtcagct ggcatttgcg gttaccgcgt tcgagcagac ttttcttctg
     1561 ctccggcccg gctttccacg gaaggaactg gcgctctaaa gagctgagct gctggctgat
     1621 cgccgactgg gacacgccat tcagcttggc ggttcgggtg aaattccggg tctcggccag
     1681 gtcgcagaac actttcaggc tctcgatttg catgagaaac tttaatactc cgtttaattc
     1741 gcgttgtcaa ccgccccgcc gtgctgaccc cgggcggcgg gcgttgcgct tcggcgtcag
     1801 gacgaaggcc gccggcagca attctttgac cgtaaaggcg cgcaccgcgc ccaacgaccg
     1861 gctgtcggcc acccagaccg cggcgcgcgg ggcgtattcc gccagcagtt gccggcaggc
     1921 gccgcagggc atcgggccgc ccgccgtgcg cgccacgacg gcgaccgcca cgaaatcccg
     1981 ctgcccggcg gtcagggcgt tgaacagcgc cacgcgctcg gcgcagcagg ttaggccgta
     2041 gctggcgctt tcgacgttgg cgccggtgat gacctggccc gagcgggtga gcagggccgc
     2101 gccgacctgg aaacgggaat acggcgccac cgcgccccgg cgcgcgcggg cggccgcccg
     2161 caccaaagcg agtttattga gtttggccat aaagctgggc gaatgtttcg agcagccggg
     2221 cggcgacgcg ctggacccgt tcggcggttt ccagcacttc cgcgtgtgag agggttgggc
     2281 ggccgcgccc ggcggcgagg ttggtgatgc aggaaatccc ggcgaccttc aggccgcact
     2341 ggcgcgccac gatggcctcg ggcacggtgc tcatgcccac ggcatcggcg cccagcctgg
     2401 caaaagcccg aatctcggcg ggcgtttcgt agctgggccc gctgaccgcc caatagaccc
     2461 cggagcgcag tttcaccccg caggcccgcc cggcccgcgc cagcaggcgc cgccagcccg
     2521 ggtcatagac gcaggtcagg tccacgaagc gcggccggcc gggcgccggc tcgccgcgca
     2581 gcgggttgag gcccataaag ttgacgtgat ccgtcagcac catgaaatcg cccgggcgga
     2641 aggaacggtt cacgcccccg gcggcgttgg tgagcagcag gtcgcggatg ccataggccg
     2701 ccagcgcgcg cacggcgaac gtgaccacgg ccatcgcgtg cccctcgtag taatgcgccc
     2761 ggccgctgag caccatgacc ggcgtgccgc ccaggcggcc gatgaccagc cgtcccgcgt
     2821 ggccgctgac gccggtcgcc ggaaagcccg gcagccgctg gtaggcgatg tccgcgtcca
     2881 cctgcagctc gctcaacacg tgttgaaagc cgctccccag caccagcgcc agagtcgggc
     2941 gcagccggga aacctggcgg agccgcgcgg cggcggtttg cgggtcgggc tggcgggagg
     3001 gctttccggg cttcggggca acggaatcat ccggccggtt gcggcggggg cgcgaacggg
     3061 agccgcgctg atttgcgctg ttcttcacgg cgcttgagtt tgcccgaaag cgggcggcgg
     3121 agccaaagga aaattcacgc ggcgggcggg gggcgcgcgg ttgccgcccg ctgctggaaa
     3181 acagattgcc gtcagcaccg gactggctta aagttctgcc gggttgcgcg agtagctcaa
     3241 ttggatagag cgtcggcctc cggagccgaa ggttgtgggt tcgacccccg cctcgcgtac
     3301 cacttcagcc cgcctggctt gggaaagagg ttgggctgca aacagcttga aaatcccccg
     3361 cgcggcgccc acactcgggg catgaagttt ttcactacga tttctgcgtt gctgatcgcc
     3421 gccctcggtt tggccccggg cgccgtgcag ggacaggccc ccgcgccggc tccggaactg
     3481 catcaatgcg tcaagcccgt cccccgcgag gggcagggct atcaacggta tgtggagttg
     3541 aaccagcgcg tccgggacag ccagggccag gcccgggtaa tcttcgtggg cgattcgatt
     3601 acgcaggggt gggagggcaa cggcaaagag gtctgggcga agtattacgc gccccgccag
     3661 gcgctgaacc tgggcattgg cagcgatcat acccagcacg tcctctggcg gctcgaccac
     3721 ggcaatctcg acggcttgca tcccaaggtc gccgtcgttc tcatcggcgt gaataatatc
     3781 cccgacacca ataatacccc ccgccaggtg ctggagggcg tgacggcggt ggtccggaag
     3841 ctccgggaga aactgcccga gacgaaggtc ctgctgctgg gcatcttccc gttccgggag
     3901 gacttctgcg accagcgcgc caaggcgctc caggtcaacc aggctttgca caagctggat
     3961 gatggccact ggatttactt ccgcgatatt ggccacttgt tcatccagcc cgatggccgg
     4021 atttccccgg ccatgatgcg cgacttcctg cacctctcgc cgaccggcta tcgtttgtgg
     4081 gccgaggcca tcgagcctga cctggcggcc ctgctcgggg agaaacccat cacgccataa
     4141 gccgcgcgcc cggcccgccg cgccggcgcc cgcgcgcaac ccggcaacgc cgggaggatt
     4201 gcgtcccgcg gttttgctgt ttccgggaaa accggacttc gctaagctcc ggccccgtga
     4261 catctgttta acggccttta tgaagattgc attgtttggc tgcgctgaca ttaaagtcgg
     4321 caagcataac cttaaagatc cccggctgga ccaggcggac aaactggtcg aagccggcaa
     4381 aaagacctat gcccaggccg atgtgattgg cgaggatcag ttgctcgaag ccgatggcat
     4441 cctggccacc caggagtcga gggcggactt gattctcaaa gacctggagt ttgtggaaac
     4501 gcgcctgggg cgcgatccgc aacccgcgga acgggcggcg ctggagaaga tcaagggatt
     4561 gctggagagc gagcggtttc tgttcaacgc gggcctggcc gccgaagatt ggcaggcggt
     4621 cgcggcgcac aacttctaca cgagcaagcc gatcacggtc gcgacgccgg cggatcggca
     4681 ggacccggat gcgctgttgc ggcgcgcctt tgccgaggcc ggctacattt gcttcctgac
     4741 ggtgggcggc aaggagaacc gcgcctggcc cattcgccgg ggcgccacgg cctgggaggc
     4801 ggccggcgcg atccacaccg acatccagaa aggctttatc cgcgcggaag tgatcagctt
     4861 tgcggacttc ctcgacgccg gcggcgagac gccggccaaa cgggcgggca agcagcgcct
     4921 ggaactcaag ccgtatgtcg tgcaggacta tgatttggtg aacttccgct tcaacaagtg
     4981 agccggccgg aggcggcgtc ccgcgccccg ccccgcgccg ggccgcgagg ctgatttccc
     5041 gctttccgtg gccgcgccgg ctggcttaga atgagaacgc tatggccttt ctgcgccgct
     5101 gcctattgat tttggccgtg ctgctcgccg ccggggcgcg gcttgccgcc gccagcgctg
     5161 ccgaccgcgc tttcggcgcc gcggtcaagg ccttccagga ttccttctac gagcgggcgg
     5221 aagcccagtt ggccgagttt agccagaagc atcccgcgtc gccgcgccgg gcggaggcga
     5281 ttttgctgca ggcccaggcg cggctgatgc tgacgaatta cgcgggcgca atcgaattgc
     5341 tttccgccca ccagggcgcg gcggggccgc tggccgacca gtatgcgttc tggctggcgg
     5401 aggcgtattc ccgcaagggc gattggcgcg ccgccagcga cgcctttgcg cggatgagcc
     5461 gggagttccc cgcctcgccg cgaggcctgg aagccgtgat cggcgaagcc tccgcgcggg
     5521 ccgcgctggc ccaggcggac ccggcggaat ggccgcgcct gatcgcgctg ctgcaggcga
     5581 ccaacggggt ctttcagacc gccgcgcgcg ccgcgggcga ttccgaactg gtggcgcaag
     5641 gctggctgct gctgggcgag gcgcagtttg cgaccgggga ttatcgcgcc gccgaggcaa
     5701 cccttcaacc gctggccggt cagctcttga aaccggaact ggcctggtca tggcaatacc
     5761 tgctctgccg catccaggtt gccgacgggc ggccggaggc ggcgctgccg ggcgcgacca
     5821 atctcatggc gctggccgcc cgcgtcgtcc cggacaaccg gcgcgccgag agcgccgcgt
     5881 tccaggccgg cttgtttgag cggttgggcc gcttcgagga tgccatcgcg gcctatcagc
     5941 agaatctcac ggacggcgtc tcccccgaac gccagcgcca ggcgttgttg aagatcagcg
     6001 aattgtatct ggcgcatgac cggatcccgg aagccaccca atcgctggag aagtttttgg
     6061 cgcagtatcc cgaagccgcg gcggcggatt tggcgctgca gacgctgggg gaattgcgcc
     6121 tccgccaata cgaaaccgcc cggggcgccc agccgttgcc cgcgccgccg gctctggcgc
     6181 cggacgcgac caataacctt cgccaggcgc gggaatcctt tgaagcactg acgaaaaggt
     6241 ttccgcaaag cccgctcttc gggaaggcgc agctcgacct gggcgaatgt tattggtggg
     6301 aggggcaaat gccggaagcg gcggcggcgt tccaggcggc ggtggaacgc ctgccccggt
     6361 cgctcgacca ggcgcgggct tgctcccggc tggcggatgc gtgttttcgc cagacgaact
     6421 acgcgggcgc catcaaatac taccaggcgg ttatcgaaca atatgccgat ctgccggagg
     6481 cgcggaccaa cctgttcgcg cgcgcgctgg atcagaccgt gcgggcgggt ttggcggcgg
     6541 gcgacctggc ggcggccacc aacgcgctcc agaaagcgct ggattggtat cccgaccgcc
     6601 cggataccgc ccgggctctg ctgttcaccg gccaggaaat cagccgccgg ggcgatccgg
     6661 cggcggcgcg gcaaatcttc cttgagttta ccaatcgcgc cccccacacc cccttgctgg
     6721 cggaggtgcg gttggccgtg gccggcacct atgagcagga acggaactgg ccggaggcga
     6781 tcgcccaata tgacgcctgg ctggccgcct tcccgaacaa cccggcccag ccgcgcgccg
     6841 aatatgcccg cgcctggaac agctttcagg ccggccgcga aaccaacgcc ctgacgagct
     6901 tcaccaactt catcgcccgg ttccccacca atgaatttgc gcccctggcc caatggtggg
     6961 tggcggatta ttactaccgc gcgggcgacc cgatggaagc ggagcggaat tacaagctgc
     7021 tttttctgaa cacgaactgg ccgccgtccg agctgaccta cgaggcgcag ttgatggccg
     7081 gccgcgccgc cgtggcccgc cagggctggg gggaggcccg gggctacttt accagcctgt
     7141 acaacaacac caacggcccc agcctggact tgcgcctgca ggccttgttt gaactcggcc
     7201 tggctctgat gcgaacggtg gatgcgtcgg aaaccaaccg gctggccaac tgcgaggaag
     7261 ccacgcgggc ttttggccgg ctgtgcgacg attatcccac caaccggctg gcggtccggg
     7321 cgtggacgga aaaggccaac tgttatttgc aatgggcgct ggcccggcag caatacgatt
     7381 ccctgaccaa tgcgctgaac gcctaccaac aggtcgttga ttcgccgctg gcggaggtgc
     7441 ctgcccgcag ccaggccaag gttggccagg ccattgtgct gagcaaatgg gccgagcaaa
     7501 agaccggcga cgaacgcacc gccctgctta aaaccgcgct cagcaactgc ctcgacgtgg
     7561 tctatggctc cctgctgcgc gagggggagc aactcgaccc cttctggacc cgggaggccg
     7621 gcattgaggc tttcggcctg gcggaggcca tgcaggcgtg gtcccaggcg gccagcgttt
     7681 atcagcggct cacgaattcg gtctggcccc agttgcccgc ctcgctggaa aagcgcgcct
     7741 ggcaggcgcg ggaaaacctg gcgcgggaaa agaacggcca ttaacttgcc tgcccatccc
     7801 ggcaacgccc gcttccgccg cccggctgcc gccccgggcc gggttggctg cgcgccgccc
     7861 gcatgaccct ttcccccgcc gccgggacgc cggccctcgg agtggttctt ctggccgcgg
     7921 gccggtccgc gcgcatgggc aaacccaagc tgctcctgcc gtggggcgac acgtcggtgc
     7981 tgggccacct gatccgccaa tggcaaagcc ttggcgtcgc gcagctttcc gtcgtttgcg
     8041 cggccgggga ttcggccctg gcggcggaat tggaccggct gggcttcccg gcggaaaacc
     8101 gcatcctcaa cccggcgacg gagcgcggca tgttcagttc gcttcaatgc gccgcgcttt
     8161 ggcggggatg gcgggcggaa gtcacgcggt gggcggtcgc gctgggcgac cagccgcagg
     8221 tgcgcctcca aacgctgcgt caggtgctcg agttcagcgc cgcgcacccc gcccgcatct
     8281 gccagcccgc gcggcgcggc cgcgggcgcc atccggtctt gctgcccgaa gcggcctttc
     8341 ggcagttggc cactgctgcg gtgtccacgc tgagggattt cctggccgcc catcccgagg
     8401 gcgtcgtttt ttgtgaggcg gatgaccccg ggctggattt ggacattgac cggccggagg
     8461 attacgaaca ggtcctccgg ctcggaggac tggtgccttg aagggcgggc cgccagcgcg
     8521 gtcgtccggc ggaggcggaa cgccctcgcc ccagccgacg caagcctgga aggagcgcgc
     8581 gcttcaacgc cgccggcgca ccgggccgaa gaaataaccg ccccgcacgc gcgcgccccg
     8641 cactcgcccc ccgccggcgg ggggctggcc cggcttgcct tgttcgaaca aggtggtgta
     8701 gcgataattg aagttttcca gcttcacgcg gggaatcttc tggccgatga aatgctcgat
     8761 ggcgttcacg tgccggacgt cctccgccac catcaaggta taggcgtcgc cggtggcctc
     8821 catgcgcccg gtgcggccaa tgcggtggac gtagtcctcc gggtgctgcg gcacgtcgta
     8881 gttgattacg tggctgacgt cggcgatgtc cagcccgcgc gcggcgatat cggtggcgac
     8941 cagcacctcg aagcggccat cgcggaagcc gcgcaacgcc tgctcgcgct cgcgttgcgt
     9001 gcggttcgag tgcagcacgg ccaccgcatg ctgattccgc ttcagcagcc ccgcgacccg
     9061 gtcggccccg tgcttggtgc ggcagaagac aatcaccgaa tcgtagttca cgcgccggag
     9121 cagttcgagg gccaggtcgg ctttctggaa gtcggacacc gggtagatca catgcttcac
     9181 cgtgtccttg ggcgagcggc gcacgccaat ttcaatcgtc tgcggctggc gcatggccca
     9241 ttggatgagc gtctcgatct ccggtgggat ggtggcggag aacagggagg tatggcggtt
     9301 gcgggggcag cgatccagaa tgcgccgcac atccggcagg aagcccatat ccagcatgcg
     9361 gtcggcttcg tccagcacca cgaactggac ccggtcgagc cggcaggtgc cgcgctgcag
     9421 atgatccagc agccgcccgg gcgtcgccac cagcacgtcc actccgtcgc gcagcgcctc
     9481 caactgcttg ccataaccga cgccgccaaa aatgaccgcc gtgcgcagat tggtgaagcg
     9541 ggcgaaatta tgcagggcgg tttccacctg ggcggccagc tcgcgggtgg gttcgaggac
     9601 gaccagccgc ggccccgggc catgcgttcc cagtttggac aggatgggca gcgcgaacgc
     9661 cgcggtcttg ccggtgccgg tttgcgcgct gccgattaca tcctttccct cgaggatcaa
     9721 cggcatggcg cggagctgaa tcggcgtcgg ctcaaggtat cccatcgccc gcactccttg
     9781 cagcaccgcg ggcgaaagtc caaacctggt aaatggcata agggtatggc gaatggcgcg
     9841 ggttgtgcgc gggacggtct atggcagcgg cccaaccggg tcggctggcg gcatctcagg
     9901 cggaacttcc ggcagattct ccggcggcgc ggccggcgga acttcgttct ccgccgtttc
     9961 cggtgcgatg gccgcttcac tggccgctgc cggggcgatt tccggggctg gtgccggcgg
    10021 cggtggcggc gtttccgccg cgggaatcgg ggcggcttcg gccggcgcgg gcttggccgc
    10081 gggcttgggt tccggtttcg gttgtggctt cttggccgtt tccaggatgc cggtggcaaa
    10141 ctcgatcaca tgcccctcgt ggatcagcca atgcaggtcg gcaatcaccg cggtctgctc
    10201 cggagtcggt tcggcgctct ccaccggcgg cgcgggcgcg gctccggctt ccgcggtggg
    10261 gactggcggc ggcgacggcg cgagggcttc aatgagttgc ttgcgagtgc acttcggggt
    10321 cgcgttgatg tattcgacga tgcgcttgac gccttcggag actggcgtgg ccgccacatc
    10381 caggtatcgc ggccgcgcga tgcaaacatg cgtcagcgtc ttgttgacct tgaagaattg
    10441 caggccgtgc cccgcgaact gctggctgag caccgtggcc aactgcagcg gaaaacgccg
    10501 ctggtcctcg cagacgctgc ggaccaggcg cgccaggtcc ggactgcgca acgcgtgggc
    10561 ggccgcgccg ttgagcgagt gggactcgac cgactggatg atgttttcct tgtggttctc
    10621 gcggaaatgc tgttcgaccg cctcccggct ggtcagccgc accggttcgg gcaggttgag
    10681 gcagacatat tcggttttcc agctccggtc atccacccac ttcttgacga cctcctcgtc
    10741 gcgcacgatg cgcacgcgcg acttgaattc ctcgaagggc atccgggcga agcgctcgct
    10801 atgcagcttg cgcaactggt tctggtaatc gtgatggttg ggcgggccga ggatgcggcc
    10861 gctcaaaccg cactgcgcga caaacgtgta tttgcccttg ggcggctcgg tcgccgtccg
    10921 ctccgcctgg tagaacgtgg caaaatgctg cttgagaacg tgggcgatgg cctcgtcctc
    10981 ggacagccag agcgtgtcgt ccagcgcgca gacaaacagg ggctggatgg gctggccctg
    11041 gggatttttc ttcacgctga acagcacctg gtgccgctcc ggcttctgga gaatcatctg
    11101 cgcgatctcg aacagcggat acgcccggcc cgtcatcttg atttgccgcg ccagggattc
    11161 cacgcctttg tcttccgggc gcaggctcgc ctcgatttcc ggcagcggac gcggcgcgcg
    11221 gcgctcgcgc ggttcgcccc ggcgaaaatc ggctttgggg ccgcccggcg cctgatcacg
    11281 cccgccctcg gggcggcgcg ggccgcgccg ctcgccgcgt gggcggtcgc cctcccgccg
    11341 cggcccggcc gggccctcgc ggcggggcgg ccgggggccg cgccgatcgc gatcccggcg
    11401 gcggtcaaat cgttccgttt cgccctcata attggcgtat ttcgaagcgg aaggctcttg
    11461 cgcccaggca ggcagaaaga gtttgttcag atcgaattcg ttatcagaca atatgctcat
    11521 tgcttcacat caggcgcttt tagcggcgcg ccttgcaagt accgctcggg cagcatggta
    11581 tggctttcgt ccgaatgcaa gcgcaggctg gcatttattc caggcgcgga agccgccttt
    11641 tttgcagag
//
LOCUS       DAOPQG010000110        11581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6420_length_11581_cov_15.776766, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000110 DAOPQG010000000
VERSION     DAOPQG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6420_length_11581_cov_15.776766"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            730..1164
                     /gene="rplM"
                     /locus_tag="PKI07_11350"
     CDS             730..1164
                     /gene="rplM"
                     /locus_tag="PKI07_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOC51679.1"
                     /translation="MKTHLPKVNLEQRKWHVIDADGAVLGRLAAQVANVLRGKDKPVY
                     TPHLDTGDFVVVINAEKVRLTGKKETAKEYMSYSGWKGGEKYRNVEQVRARHPERLIT
                     HAVRGMVPKNRLGRVLMTKLKVYKGSEHPHAAQRPQVLAPAK"
     gene            1193..1609
                     /gene="rpsI"
                     /locus_tag="PKI07_11355"
     CDS             1193..1609
                     /gene="rpsI"
                     /locus_tag="PKI07_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOC51680.1"
                     /translation="MSDIKAPEVKTTQNLGTGRRKTAVARVRLSTGAGKILVNGRPFE
                     NYFPLESLRVVATQPLTVTGSTGKFDAHITVSGGGPSGQAGAVRHGLARALLAMDANL
                     RPLLKAEGLLTRDARQKERKKYGQPGARKRFQYSKR"
     gene            1664..2698
                     /gene="argC"
                     /locus_tag="PKI07_11360"
     CDS             1664..2698
                     /gene="argC"
                     /locus_tag="PKI07_11360"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOC51681.1"
                     /translation="MNAKPVAIVGASGYSGEELVRLLLAHPHVELAAVTSRQYAGRTL
                     AQIFPRFAHHPRARTLRFSEPNPAVLAKQAQVVFLALPHGVAAEYAVPLLEFGCQVID
                     LSADFRVRSAAVYQEFYAHEHPAPELLARAVYGLPEIYRAQIKPASLIASPGCYPTSI
                     LLPTLPLLKAGLVAPEGIIANSLSGVSGAGRKVELDYLFVECNESARPYGIPKHRHLS
                     EIEQELSLAAGRPVTIQFTPHLIPVNRGILTTLYLTPAGATLEQITECYQAAYAREPF
                     VRLLEGKALPDTKNVAHTNVIEIAWRHDPRTGRLIVMSAEDNVVKGAGGQAIQSMNLM
                     CGFPETAGLM"
     gene            2776..4101
                     /gene="argJ"
                     /locus_tag="PKI07_11365"
     CDS             2776..4101
                     /gene="argJ"
                     /locus_tag="PKI07_11365"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOC51682.1"
                     /translation="MKTPLTPLPGSVTAPRGFRAAGVFCDIKRLGTGKGSNKGKKRDL
                     ALIVSDVPATAAGMFTTNQACAAPVKVCLEQLKRGRAQAVVVNSGNANACTGKQGLQD
                     AREMARFTEATLALPPGAALVGSTGRIGVAMPMDHVRAGIIEAALGLGTTAAHAAQAA
                     EAILTSDTHAKQVAVQFKLGGRTVRLGGICKGAGMIQPGMSATGRRPAATPRHATMLC
                     FLTTDAAVEAQALQAALREAVAHSFNRITVDGDMSTNDTVLVLANGRAGNRPLGVSAP
                     GFPQFQAALSHACLALARMIVRDGEGVSRCVTVRVQGARSFADADAAARAVANSPLVK
                     TSWHGGDPNWGRILAAIGYSAATIEESKVDIGYSAPGSRRILWSLRRGQPTAATFKAL
                     CAAVAPREFDLHVNLNIGRARAVIYAADLTEAYVDFNKGDVSDPSSLGG"
     gene            4370..5209
                     /locus_tag="PKI07_11370"
     CDS             4370..5209
                     /locus_tag="PKI07_11370"
                     /inference="COORDINATES: protein motif:HMM:NF021354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HOC51683.1"
                     /translation="MEIKRGAAPPPPRARGIRLVRMRRLVQFAFLLAVAGGAGRTAAE
                     APPRPLAAAVYEGAGATGKGVPRVLALLEATPGVTATRIRPEEIRAGTLRRFDVVVFT
                     GGSGHGQAQALEPAGREEVKTFVRQGGGYVGICAGSYLACCGFSWGLNLINAKTLSPL
                     WKRGKGTVQIELTDRGREILGPFPGKLDCLYAQGPIVGPAHARDLPVYEPLAFFRSEI
                     AKNNTPQGIMIHSPAIFASHFEKGRVLCFSPHPEQTAGLDRLVTQAIIWAGTPARQNG
                     TGP"
     gene            5345..6928
                     /locus_tag="PKI07_11375"
     CDS             5345..6928
                     /locus_tag="PKI07_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOC51684.1"
                     /translation="MPPLRASRREFLKASALYAGALALAPLARGQAASARAMAPPPRC
                     LHPYPNQIAWGSGVQSLRRQVTLAVGRDADAGAVALMKDTWQRFTLGSVKLKLARAAE
                     LDASGFSLAGGRPPPLLPNATYALTVDAAGIAARGKDAAGLRHAWFTLLQLLEPAETA
                     AGGLEFTLPQAAIQDWPALPFRGLHLCVFPETAPVMLEKAIRLAAFYKFTHVALEFWG
                     QLRLHALRELAWPGAWTKARAGRLIAMARGMGLEVIPMFNCWGHASASRLKHGRHVVL
                     DQNPRLAPLFEPDGWTWCLSNPRTQALLRSVCDELIAVAGPGRYFHIGCDEAYSHATC
                     DRCRRADPVQLFAGHVNQLAAHLEQRGRRAIMWGDALLERGKWPAGFTANGTAAQPTH
                     EALDRLSHRIVIADWHYNVTQGDVPTLAHFRRHGFAALACPWNSAGNIRALARAAAAS
                     QSGLLMTTWHHLAQSIPLLAWTANCAWSANHAALGLRQMDGALMRTASASHLRRLAPA
                     RGQFKRAGWNPFEQPAEVD"
     gene            7015..8433
                     /locus_tag="PKI07_11380"
     CDS             7015..8433
                     /locus_tag="PKI07_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOC51685.1"
                     /translation="MPVPVISIEQMRDWERATWSAGQTEAEVIRRVGQCVARRALALT
                     QPGDRILILAGRGHNGEDARAAREHLASRTVEMLDAAGAAADLEKLNALLKLRPALLV
                     DGLFGIGLNRPLGPEWISLIERVNEARIPTLAVDVPSGLNADTGQPQGAAVKATVTLT
                     VGAPKTGLLAETAWPFVGRLEVASEVGLTPCPHQSELQWTLPEDFAAFPPARTAATHK
                     GSYGHLAIVAGSMGYHGAAVLASRGAQRARPGLITLHTLESVYYAVAPQLDAVMVAIW
                     SPASKLTSSYSAILAGPGLAAADIPDPLKMTTRHLWRDALLPVVVDASALDWLPLDPV
                     PRNAVRVITPHPGEAARLLRGTPARVQANRLAALRDVSRRFGNSWVVLKGHQTLIGRS
                     TGEVYINSSGNPYLAQGGSGDVLAGYLAGLVAQPDLQADPLLAIRYAVWQHGAAADHL
                     QALRANWEMADLAAMLGTIKCQ"
     gene            8975..9961
                     /locus_tag="PKI07_11385"
     CDS             8975..9961
                     /locus_tag="PKI07_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOC51686.1"
                     /translation="MTGEAVIGLDLGGTKLAGAIYRPNGRIAGKNVVPLAGRQGRAVG
                     ELIGSELANLFAGARRQGLQVAALGVSVPGISHSDTGTVWAPNIPGWEDYPLRRELRA
                     AVPNKRIRIAIDSDRACYILGETWQGAAKGCRNAIFLAVGTGIGAGILIDGRILRGAH
                     DIAGAIGWLALNRPFRRQYVSCGCFEHHASGRGLAKVAKEILARRARERTACGTPGLR
                     NRRGLTAQDIFAAYDCGDPVAKEVVAQAIEFWGMAAANLVSLFNPEKIIFGGGVFGPG
                     SKLLSRIHAEARKWAQPISIGQVKLLASKLGGDAGLYGAGCLAWQAANPAKT"
     gene            10130..11332
                     /locus_tag="PKI07_11390"
     CDS             10130..11332
                     /locus_tag="PKI07_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51687.1"
                     /translation="MYNRNLVFAAACLGMLLFGIVFLSLGSAMNMLAERFQLDNNSIG
                     TLTALLPFGILAGSLIFGPIVDRFGYRWMLIVCSLLVMAGLEGMAFGHREGHIQFFIF
                     LIGLGGGVLNGATNALAADVSEGARGARLSLLGVFYGIGALGMPSTLAALSRHFSLAG
                     IIAGIGVFVLVPVVYFLVIPFPPPKQQARSGTLSSGLALLRHPFFLLAGLALAIQSGM
                     EGMTNDWITRYFKHVTLAGRPAEEWRTLLGLMVVTGAMVLMRIVLSGLLQHTRSSRVL
                     FVSIGITAAGGLLLLFAGGYGAALAAAALIGAGLAAAFPVVLGYIGDRYPAQSGTAFS
                     AIFFIALIGNMTINKSFGFLAQSHGIQQYPKVMLGCLLASLILLSLVVKQLPGRTAPA
                     AAPETIPS"
     gene            11370..>11581
                     /locus_tag="PKI07_11395"
     CDS             11370..>11581
                     /locus_tag="PKI07_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOC51688.1"
                     /translation="MSTIAQQWLTNTRAVMDRIEQTQLDAIRQAAGIMADTIQAERWV
                     HTFGCGHATIPIEEMYPRIGGFVGFHP"
BASE COUNT         1879 a         3890 c         3867 g         1945 t
ORIGIN      
        1 tctcagcaag ctactgaaaa ggcaccagtt aagaaagcga agcccgcctc cacctcgccc
       61 tcttccactg cccgcgctac cgtttcttct tcgtcgtttc aggctatgta caaggggagt
      121 acaccggagg cgcggcagga gcgcatggaa aataagcatg aacccccttt gagcatcctt
      181 ggtacacccc ttgtacacgg cctattggga gccataggct ggctggggag gttgaggatg
      241 ggttgggcgc tggcctgagc cgcattggag caactggctg cccggagaat atgtaaaacg
      301 acgttaaaat aaaggttggt ccaggccagc gtgcgccgtg cccgaagtca cggattcgcg
      361 cggatggact tgctgcctgc cggttgcctg gttcggcggg cgggtagcgg ctccggggcg
      421 cacatgaggg catgagcttg cgcgggccgg ccgcggaccc gcatccagcg aacccgattc
      481 gggaaggcga acgcctccgc gcggcctggc ttcccgaagc cctgaaatcc ggacgggcga
      541 aggatcgcgt ccctccgggc cgggttagcc aggcattagc gtttcccctt tgcagcgggg
      601 cgtggatttt tttcggccgc ccgccggggg ccggggcggg cggcgggcca caacgaaaaa
      661 ccgtagagat gcctgttgac ttggcgggcg ggtctgctat gttgcgcctt cgtttaacca
      721 ggattttaga tgaagacaca tctgccgaaa gtgaatttgg aacaacgcaa atggcacgtg
      781 attgacgccg atggcgccgt gctgggccgc ctggcggcgc aagtcgccaa tgtgctgcgc
      841 gggaaggaca agcccgtcta tacgccgcat ctggacaccg gcgattttgt cgtggtcatc
      901 aacgcggaga aggtccggct caccggcaaa aaggagacgg ccaaggaata catgtcctac
      961 tccggctgga agggcggcga gaagtaccgc aacgtcgagc aggtgcgcgc ccggcatccc
     1021 gagcggctca tcacccacgc ggtgcggggc atggtgccca agaaccgcct gggacgggtg
     1081 ctgatgacca agctcaaagt gtataaaggc agcgagcacc cgcacgccgc gcaacgtccg
     1141 caagtcctgg cccccgcaaa gtaagaacct taacccccca cttttttcag gcatgtcaga
     1201 tataaaagct cccgaagtca agaccacgca aaacctcggc accggccgcc gcaagacggc
     1261 ggtcgcgcgc gtgcggctat ccaccggcgc cggcaagatc ctggtcaatg gccgcccgtt
     1321 tgaaaattat ttcccgctcg agtccctgcg cgtggtggcc acgcagcccc tgaccgtgac
     1381 cggctcgacc ggcaagtttg acgcgcatat caccgtttcc ggcggcggcc cgagcggcca
     1441 ggccggcgcg gtgcggcacg ggctcgcgcg cgccttgctg gcgatggacg ccaacctgcg
     1501 gccgctgctg aaagcggagg gcctgctgac ccgcgacgcg cgccagaagg agcgcaagaa
     1561 gtacggccag ccgggcgcgc gcaaacgttt ccagtacagc aaacgttaat cgcgctggat
     1621 attttcccgc cccgctggtt caatccggcg gggttttttt atgatgaatg ccaaaccagt
     1681 agccatcgtg ggcgcctcgg gatactcggg cgaagaactc gtccgcctgc tcctggcgca
     1741 cccgcacgtt gaactcgccg ccgtcacctc gcgccaatac gccgggcgga cgctggcgca
     1801 aatcttcccc cgcttcgccc atcatccgcg cgcccggacg ctgcgcttca gcgagcccaa
     1861 ccccgcggtg ctggcgaagc aggcgcaggt ggtgtttctg gccctgccgc acggcgtggc
     1921 cgccgaatat gccgttcccc tgctcgagtt cgggtgccag gtgattgacc tgagcgcgga
     1981 tttccgcgtg cggagcgccg cggtttacca ggagttctat gcgcacgagc accccgcgcc
     2041 ggagctgctg gcccgggcgg tttatggcct gccggaaatc tatcgcgccc aaatcaagcc
     2101 ggcctcgctg atcgcgtcgc cgggctgcta cccgaccagc atcctgctgc caacgctgcc
     2161 gctgctcaag gcgggcctgg tcgcgcccga gggcatcatt gccaattcgc tgagcggggt
     2221 gagcggcgcc ggccggaagg tcgagcttga ttacctgttt gtcgagtgca acgaaagcgc
     2281 ccggccctac ggcatcccca aacaccgcca tctctcggag atcgagcagg agctttcgct
     2341 ggccgcgggg aggccggtga cgatccagtt caccccgcac ctgatcccgg tgaaccgcgg
     2401 catcctgacc acgctgtacc tgacccccgc cggcgcgacg ctggagcaga tcacggaatg
     2461 ctaccaggcc gcctatgccc gcgagccgtt cgtgcggctg ctggaaggca aggcgctgcc
     2521 ggacaccaag aacgtcgcgc ataccaacgt gatcgaaatt gcctggcggc atgacccgcg
     2581 cacggggcgg ctcatcgtca tgagcgccga ggataacgtg gtcaagggcg ccggcggcca
     2641 ggccatccaa agcatgaacc tgatgtgcgg tttcccggaa accgcgggcc tgatgtgaga
     2701 cggcgcggcg ccgccccgcg cgcgcgcagc gcccggcccg gcccgccctc cccctgcctg
     2761 attccgattg ttgccatgaa aaccccatta actcccctcc cgggttccgt caccgccccg
     2821 cgcggcttcc gcgcggcggg cgtcttctgc gatatcaagc gcctcggcac cggcaaaggt
     2881 tcgaacaagg gcaaaaagcg cgacctggcg ctcattgtct ccgacgttcc cgccaccgcg
     2941 gccggcatgt tcaccaccaa ccaggcctgc gccgcgcccg tcaaggtctg cctcgaacaa
     3001 ctgaagcggg gccgggccca ggcggtcgtg gtcaactccg gcaacgccaa tgcctgcacc
     3061 gggaagcagg ggctgcaaga cgcccgcgag atggcccggt tcacggaagc gacgctcgcg
     3121 ctgccgccgg gcgcggccct ggtcggttcc accggccgca tcggggtggc gatgccgatg
     3181 gaccacgtcc gggccggcat catcgaagcc gcgctggggc tgggaaccac cgccgcccat
     3241 gccgcccagg ccgccgaagc catcctgacc agtgataccc acgccaagca ggtggccgtc
     3301 cagttcaagc tgggcggccg gaccgtccgc ctcggcggca tttgcaaagg cgcggggatg
     3361 attcagccgg gcatgagcgc caccggccgg cgcccggcgg ccacgccgcg gcacgccacc
     3421 atgctgtgct tcctcaccac cgatgccgcc gtcgaggccc aggccctgca agccgccctg
     3481 cgcgaggccg tcgcccacag ctttaatcgc atcacggtgg atggcgacat gagcaccaac
     3541 gacaccgtgc tcgtgctggc caatggccgc gcgggcaatc gcccgctcgg cgtgtccgcc
     3601 ccgggctttc cgcaatttca agccgccctg agccacgcct gcctggcgct ggcccggatg
     3661 attgtgcgcg atggcgaggg ggtatcgcgc tgcgtcaccg tgcgcgtcca gggggcccgg
     3721 tcgttcgccg acgccgatgc cgccgcgcgg gccgtggcga acagcccgct ggtcaagacc
     3781 agttggcatg gcggcgaccc caactggggc cgcatcctcg ccgcgatcgg ttatagcgcg
     3841 gcgacgatcg aggaaagcaa ggtggacatc ggctacagcg cgcccggcag ccgccggatt
     3901 ctttggagcc tccggcgcgg ccagcccacg gcggccacct tcaaggcgct ctgcgcggcg
     3961 gtggcgccgc gggaattcga tctccatgtc aacctgaata tcggacgggc ccgggcggtc
     4021 atctacgccg ccgacctgac ggaggcgtat gtggacttca acaagggcga cgtgagcgat
     4081 cccagctcgc tgggaggctg acgccggcca ttcccggcgc ggataagcct cccgcggttc
     4141 ccgcggcggg cttgtgcgat ggatcgcgcc gccgggttcc cgcctcccca gcccggaaga
     4201 acgcgcccgg gttgcccccg gcaaagttct tgacgcgcgg acgggttccc gacagggtcc
     4261 gagcatgatg ggtgagtgcc agagtccatt cctcctcacc ggcgtggtgc gcgcgtggca
     4321 tctgccgccc aggctccctt ccggcgcctc cggcccacgg gttgccgcca tggaaatcaa
     4381 gcgcggcgcc gcgccgccgc cccctcgcgc ccgcggcatc cggcttgtgc ggatgcgccg
     4441 tctcgtgcaa ttcgcgtttc tgctggcggt ggcgggcggg gccggacgaa ccgccgccga
     4501 agcgccgccc cggcctctcg ccgccgcagt ctatgaaggg gcgggagcga cgggcaaagg
     4561 cgtgccgcgc gtgctggctt tgctggaagc caccccgggc gtgaccgcca cgcgcatccg
     4621 cccggaagaa atccgggccg ggacgctgcg gcggtttgat gtggtcgtat tcaccggcgg
     4681 cagcggccac gggcaagccc aggcgctgga gccggcggga cgcgaggagg tgaaaacctt
     4741 tgtgaggcag ggcggcgggt atgtgggcat ttgcgccgga tcctacctgg catgctgcgg
     4801 tttctcctgg gggctgaacc tgatcaacgc caaaacgctt tccccgctct ggaaacgcgg
     4861 caagggcacg gtccagatcg agttaactga ccgcggacgg gagattctcg ggccgttccc
     4921 gggaaagctg gattgcctct atgcccaggg ccccatcgtc gggccggctc acgcgcggga
     4981 tttgccggtc tatgagccgc tggcgttctt ccgctccgaa attgcgaaga ataacacccc
     5041 ccagggcatc atgatccact cgccggccat tttcgccagt cacttcgaga aagggcgagt
     5101 tctgtgcttc agcccgcacc cggaacagac ggcgggcctg gatcgcctgg ttacgcaagc
     5161 cattatctgg gccgggaccc ccgcccgcca gaatgggacc ggcccgtaac cgaggccgtc
     5221 tccctgcgga gtttccggcg aagccccacg cccgggctcc gcgagactga cgccgccggt
     5281 ttgccggctt gcgtcggcgc aagccgcggg ccagtttgac cgcaaccaaa tcatgaaaac
     5341 ttcaatgcct cctcttcgcg cttcgcgccg cgagtttctg aaagcctccg ccctctacgc
     5401 cggcgcgctg gccttggccc cgctggcccg cgggcaggcg gcgtccgcac gggcgatggc
     5461 cccgccgccg cgctgcctgc atccctaccc taaccaaatt gcctggggct ccggcgtcca
     5521 aagcctccgc cgccaggtga cgctggcagt cgggcgcgac gccgacgccg gcgcggtggc
     5581 cctgatgaag gatacctggc agcggtttac cctgggatcg gtgaagttga agctggcgcg
     5641 cgcggcggag cttgatgcca gcgggttttc actggccggc ggccgcccgc cgccgctgct
     5701 ccccaacgcc acttacgcgc tgacggtgga tgccgcgggc atcgccgccc gcggaaagga
     5761 cgcggccggc ctgcggcacg cctggttcac cctgctgcaa ctgctggaac ccgccgaaac
     5821 cgcggcgggc gggctggagt tcacgctgcc gcaggccgcc atccaggatt ggccggcgct
     5881 gccgtttcgc gggctgcacc tttgcgtctt ccccgagacg gcgccggtga tgctcgaaaa
     5941 agcgatccgg ctggcggcgt tctataaatt cacgcatgtg gcgctggagt tctggggcca
     6001 gttgcggctc cacgcgctgc gggagctggc ctggcccggg gcctggacga aagcgcgggc
     6061 gggccggctc attgcgatgg cgcgcggcat ggggctggaa gttattccca tgttcaactg
     6121 ctggggccac gccagcgcca gccgcctcaa gcatgggcgg cacgtcgtgc tggatcagaa
     6181 cccgcggctg gcgccgctgt tcgagccgga cggctggacc tggtgcctga gcaacccgcg
     6241 cacgcaggcg ctgctccgga gcgtgtgcga tgagctgatc gcggtggcgg ggccggggcg
     6301 gtatttccat atcgggtgcg acgaggctta ttcgcatgca acgtgcgacc gctgccggcg
     6361 ggcggacccc gtgcagttgt tcgccgggca cgtcaaccaa ttggccgccc acctggagca
     6421 gcgtggccgc cgcgccatca tgtggggcga cgcgctgctg gaaaggggaa agtggccggc
     6481 cgggttcacc gccaacggca ccgccgcgca gccgacccat gaggcgctgg accgcctgtc
     6541 gcaccggatc gtgattgccg actggcacta taacgtcacc cagggcgacg tgccgacgct
     6601 ggcgcatttc cgccgccacg gttttgcggc gctggcctgc ccgtggaatt cggcgggcaa
     6661 catccgcgcg ctggcccggg ccgccgccgc cagccagagc gggctgctga tgaccacctg
     6721 gcaccacctg gcccagagta ttcccctcct ggcctggacg gcaaactgcg cgtggtcggc
     6781 aaaccatgcg gcgctcggct tgcggcaaat ggacggggcg ctgatgcgca ccgcctccgc
     6841 cagccatctg cgccggctcg cgcccgcgcg gggccaattc aaacgggccg gctggaatcc
     6901 gtttgagcag cccgccgagg tggattaagc ccgcggatta aaccgcccgc ggcgacgccg
     6961 gggcgcgggg gtttttactt tcgccccggg cgcgagcgtg tatcttcctg cgccatgccg
     7021 gtaccggtaa tcagcattga gcaaatgcgc gactgggagc gggccacgtg gtccgccggc
     7081 cagaccgagg cggaggtcat tcgccgcgtc ggacagtgcg tcgcgcgccg cgccctggcg
     7141 ctgacgcaac ccggcgaccg gatcctgatc ctggccggcc ggggccacaa tggcgaggac
     7201 gcgcgcgcgg cgcgtgaaca cctggcttcg cgcacggtgg aaatgctgga cgcggcggga
     7261 gccgccgccg acctggaaaa gctcaacgcg ctgctgaaac tgcggccggc cctgttggtg
     7321 gacggtttgt tcggcatcgg gttgaaccgt ccgctggggc cggagtggat aagcctcatc
     7381 gagcgcgtga acgaggcgcg aatcccaacc ctggcggtgg acgtgccctc cggcttgaac
     7441 gccgacaccg gccaaccgca gggggcggcg gttaaagcca cggtgaccct gaccgtgggc
     7501 gcgcccaaga ccggcctgtt ggcggagacg gcgtggccgt tcgtcgggcg gctggaagtc
     7561 gcgtcggagg ttgggctgac gccgtgcccg caccaaagcg aactgcagtg gacgctgccg
     7621 gaggacttcg cggcattccc gccggcgcgg acggcggcga cgcataaagg aagctatggc
     7681 cacctggcca tcgtggcggg gagcatgggg tatcatggcg cggccgtgct ggccagccgc
     7741 ggggcgcaac gggcgcggcc cggcctgatt acgctccata cgctcgaatc ggtttattac
     7801 gcggtcgcgc cgcagctcga tgccgtcatg gtcgcgatct ggtccccggc cagcaagctg
     7861 acgagctcct acagcgccat cctggccggc cccggcctgg ccgccgccga tattcccgac
     7921 ccgctgaaaa tgacgacgcg gcacctgtgg cgggatgcgc tgctgccggt ggtggtggat
     7981 gccagcgcgc tggactggct gccgctggac ccggtgccgc ggaatgccgt gcgcgtcatc
     8041 acgccccacc cgggggaagc cgcgcggttg ctgcgcggga cgcccgcgcg ggtgcaggcg
     8101 aaccggctgg ccgcgctgcg cgatgtctcg cgccggtttg gcaactcctg ggtggtgctc
     8161 aaggggcacc agacgctcat tggccggagc acgggcgagg tgtatatcaa ctcctccggc
     8221 aacccgtacc tggcgcaagg cgggagcggg gatgtgctgg cgggctacct tgccggcctg
     8281 gtggcgcaac ccgatttgca agccgacccg ctgctggcaa ttcgctacgc ggtatggcag
     8341 cacggcgcag cggccgacca cctccaggcc ctgcgcgcca actgggaaat ggccgacttg
     8401 gcggcaatgc ttggcacgat aaaatgccag taggctcccc tgccccgccc gcgggcttca
     8461 tccgcgcggt ggaatcaggc gccctcccga agcctggctt gccttctcgg cgcggacttc
     8521 gcaccttttc cacatcatcc cgggctcaat taaagccatg ctcggcccgc gcactacggg
     8581 aaaccctgga tgccttccct tgataccttt cttgcacatc gtataacgat ggtggcaggg
     8641 caaggaggtt tgggaacatg agggcgagag caaacgctga tgaagaccgc gaattgaggt
     8701 tgctttatct gacatcgccc gggctgaggc ttgcggttgg cactcgcctg atttttcgct
     8761 acagcttgct tcgcgcggca gcctggctta gctatatccc aaaaccgatg cggccccctc
     8821 atatcaatat tactctcgac cgcaacgcaa aagcagcggc agcggcggcg tattgggctg
     8881 ggggggcagc ccctcaagag actcacgaga aacccgtccc tgggcgcccg ggcgtgaacc
     8941 tgggctgccc gcgtagaaat ggagttgatt gcttatgacc ggagaggctg tgatcggact
     9001 cgatttgggt ggcaccaagc tcgccggggc gatctatcgt cctaatggcc ggattgccgg
     9061 caaaaacgtc gttcccctgg ccggacgtca gggtcgcgcc gtgggcgaac tcatcggttc
     9121 ggaactggcc aaccttttcg ccggggcacg ccgccagggg ctccaggtgg cggccctcgg
     9181 cgtctcggtc ccgggtatca gccattccga taccggcacg gtttgggcgc ccaatatccc
     9241 cggatgggag gattacccgc tgcgccgcga actgcgagcc gccgtcccga acaaacgcat
     9301 tcgcatcgcc atagacagtg atcgcgcctg ctacattctg ggggagacct ggcagggcgc
     9361 ggccaaaggc tgccgcaacg cgatctttct ggctgtcggc acgggcattg gcgccggcat
     9421 cttgatagac ggccggattt tacgcggagc gcacgacatc gccggcgcga ttggctggct
     9481 ggccctgaac cggccgtttc ggcgccaata cgtgtcctgc ggttgtttcg agcaccacgc
     9541 gtcggggaga gggctggcca aagtggcaaa agagattctg gcgcggcgag cccgcgagcg
     9601 aactgcttgc gggacgccgg gcttgcgaaa ccgccgggga ttaaccgcgc aggatatttt
     9661 cgccgcctac gactgcgggg acccggtggc caaagaggtc gtggcccagg ccatcgagtt
     9721 ctggggcatg gcagcggcca acctcgtcag cctgttcaac ccggagaaaa ttatcttcgg
     9781 cggcggcgtg tttggccccg gcagcaagct gcttagccgc atccatgccg aggccaggaa
     9841 atgggcgcag cccatcagca tcggccaggt aaagctcctg gcctccaaac tgggcggaga
     9901 cgcggggctt tacggcgccg gctgcctggc gtggcaagcg gcaaacccgg ccaaaactta
     9961 agcgcccgga cggaaaactg cccggaagcc gcgtctcgaa ccgggccgcc tccagccggg
    10021 gaaatgcttt gccccgggga cggcaaccga atgcagggat gcccgcaccc gctgcggtcg
    10081 ccggttttgg ggtttccctg gcctctcgca tggtataagg tgcgggcgca tgtacaaccg
    10141 caacctggtc ttcgctgccg cctgcctggg aatgctgctc tttggcattg tttttttatc
    10201 cctgggttca gcgatgaaca tgctggcgga gcgtttccaa ctcgataata acagcatcgg
    10261 caccctgacc gcgctgctgc cctttggcat cctggcgggc tcgctcatct tcgggccgat
    10321 cgtggaccgg ttcggctacc ggtggatgct gatcgtgtgc tccctgctgg taatggcggg
    10381 gctggaaggc atggccttcg gccatcgcga gggccatatc cagttcttta tctttctgat
    10441 cgggttgggc ggcggcgtgc tcaatggcgc gaccaacgcc ctggcggcgg atgtcagcga
    10501 aggcgcgcgc ggcgcccgat tgagtttgct gggcgtcttc tacggcatcg gcgcgctcgg
    10561 gatgcccagc acgctggcgg cgctttcccg gcacttttcg ctggccggca ttattgccgg
    10621 gattggcgtg ttcgtgctcg tgccggtggt ttatttcctc gtgatcccct tccccccgcc
    10681 gaagcagcaa gcccgatccg gcaccctgtc aagcggactg gcgctgctcc ggcatccctt
    10741 ctttctgctg gcggggctgg ccctggcgat tcaaagcggc atggaaggca tgaccaacga
    10801 ctggatcacc cgctacttta agcatgtgac gctcgcgggc aggcccgcgg aggaatggcg
    10861 gacgctgctc ggattgatgg tcgtgaccgg cgcaatggtt ctcatgcgca tagtgctcag
    10921 cggcctgctt caacataccc ggtcgtcgcg ggtgcttttt gtcagcatcg ggattaccgc
    10981 cgcgggcggt ctgctgctgc tgtttgcggg cggctatggc gcggcgctgg cggcggcggc
    11041 gttgatcggg gcgggtctgg ccgcggcgtt ccccgtggtg ctgggctaca tcggcgatcg
    11101 gtatcccgcc caatcgggca ctgcgttcag cgccattttt tttatcgccc tgatcggcaa
    11161 catgacgatc aacaaatcct ttgggttcct ggcccaaagc cacggcatcc agcaatatcc
    11221 caaagtcatg ctcggctgcc tgctggcttc gctgatcctt ctttctctcg tcgtcaaaca
    11281 actcccgggc cggaccgccc cggcggcggc tccggaaaca attccctctt aaacaccatc
    11341 acctgttaat tcctccattc cttactgtta tgagcaccat cgcccagcaa tggctaacca
    11401 acacccgcgc ggtcatggac cgcattgaac aaacccagct cgacgccatc cgccaggccg
    11461 ccggaatcat ggccgacacc atccaggcgg agcgctgggt ccataccttt ggctgcggcc
    11521 acgccaccat tcccatcgag gaaatgtatc cgcgcatcgg cggctttgtg gggttccacc
    11581 c
//
LOCUS       DAOPQG010000111        11347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6573_length_11347_cov_14.007705, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000111 DAOPQG010000000
VERSION     DAOPQG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6573_length_11347_cov_14.007705"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..1172
                     /locus_tag="PKI07_11400"
     CDS             393..1172
                     /locus_tag="PKI07_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOC51689.1"
                     /translation="MKLLFIADIVGQPGRRAVKELLPQLRQEHGVGVVVANGENAAGG
                     SGITGKTAEEIFAAGVDIITCGDHLWDQKEVMELLAGEPRFVRPLNYPPGTPGQGSAI
                     FAAPGLPPLAVLNLQGRTFMPPLENPFLAARAEVERLRQQTRLIFVDFHAEATSEKIA
                     LARMLDGQVSAVIGTHTHVQTADEQIFPGGTAFLSDGGFTGPHESVLGREIEPVIQRF
                     LTSMPQRFEVAKARVLLQGAVVEIEEQTGRAQSIQRVSCSL"
     gene            1318..2730
                     /gene="xseA"
                     /locus_tag="PKI07_11405"
     CDS             1318..2730
                     /gene="xseA"
                     /locus_tag="PKI07_11405"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOC51690.1"
                     /translation="MARISKSQWEFDGLFPSEPARKVLTVSELTWQVRRLLEQQVGEV
                     WVTGEITNRRLQSSGHIYFTLKDAGAQLSCVLFRGETQADRALVQDGRKVNLRGEVTV
                     YEPRGQYQLRVMKVELQGVGALQAAFEKLKQKLKAEGLFAPERKRPLPRYPLRIGVAT
                     SPTGAALRDVLHVVERRHPALELILAPCRVQGEGAAAEIAAAVRLLNEFNRAAAAAGD
                     RSGGVDLILVTRGGGSLEDLWAFNEEVVARAIFESALPVVSAVGHEIDFSISDFVADV
                     RAATPSAAAEIITEGVFASGQFLSEAPRRLRQSAAEQWEDKRRDWAEAAQRLARMHPR
                     RRLNDWLQRLDDAQTGLLRCLRQGMRQQRQGWHNLAERLWRVRPAALWRQKRDGVQLA
                     EQRLREQGRRLLEARRAQLDALAARLRLLGPEQVLARGYSITSDAASGKVLRAADEVK
                     PGQRLRTRLKTGEILSQAEG"
     gene            complement(2731..5214)
                     /locus_tag="PKI07_11410"
     CDS             complement(2731..5214)
                     /locus_tag="PKI07_11410"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOC51691.1"
                     /translation="MKLIIRVGQCLGFGVLALAGSLGAQTPGVFQTNLTLRIMAANLT
                     SGGPTYQTAGLNILKGLKPDIVAIQEFNYGNNSAADFRHMLDTTFGTNFVYYRESGYS
                     IPNGIISRFPIRAAGSWDDSLVPDRGFAWARLDVPGSNDLYVVSVHLYSSGSASDRNQ
                     EAQALKNLISANFPANAWVVVAGDFNTATRSEAAVSTFKTFLSDNPIPTDAESGGKME
                     TNEPRNKPYDYVLPSHSFATNFVPTVLPSRVFSNGLVFDSAVYTPLSDVAPVAPGDSH
                     VSGMQHMAVVKDFNITYTITNAWPDAPFIITQPASQIAAPGSDVAFSVAASGAGALHY
                     QWRFGAANLAGATTSMLALTNVQSGQAGDYSVVITNFAGSVTSAPASLTIAAAPVIVA
                     QPESQTVAPGQSAVFTVTATGTMPLFYQWRFNGLPLPGATGSSYTRANVQPGDAGNYS
                     VVITNLAGSVVSTEALLTVAVASGHIIAQWNFNSPTPDGQTTTGTLTPSIGSGTASYI
                     GGTKAPGSGEFAKGSAKDPQTSDNSAWNTSSYPAATIGNKTAGTLFTVSTVGWQDISI
                     AWDQRPSSTGSKYMRLRYTTNGADFIDFPTGISVARDDWESKTNSLGTLAGANNNPDF
                     AFQIVAEFESTATGAGGNNYVAAKSGSTYAPGGTVRFDMVTVTGLPIPVSVPPTITAP
                     PVSQRVQQGADATFSVLATGTAPLSYQWRFNATPLSGATASVYTCNHVQPADQGAYSV
                     VVSNSAGTVISPDAQLELVVPAPVLVMRSPQVVAWQGLSNLPYVVQAKTNLAQSNWVM
                     VGTASSPGTDVSFTNSVAAPQMFFRVRYP"
     gene            5463..5675
                     /gene="xseB"
                     /locus_tag="PKI07_11415"
     CDS             5463..5675
                     /gene="xseB"
                     /locus_tag="PKI07_11415"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOC51692.1"
                     /translation="MRKLEALVVAMESEDLSLEALLVKYEEGTRLVKACQDKLTKAEA
                     RIRQLEKTAAGEMKLQPLALPEDARE"
     gene            5747..7741
                     /gene="dxs"
                     /locus_tag="PKI07_11420"
     CDS             5747..7741
                     /gene="dxs"
                     /locus_tag="PKI07_11420"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOC51693.1"
                     /translation="MSRYLEMVDSPAHVKKLTLEQLTQLAEEIRQELITVLAKHGGHL
                     GPNLGVIELTLALHYVFNTPRDKFIWDVSHQVYVHKLLTGRKHRFDSIRSTGGLSGFA
                     VRAESEHDCYGAAHAGTALSAALGICAGRDQRGSDEHVVAIFGDGALTNGISFEALNN
                     IAPTTKRFIGILNDNEWGIAKNVGAIANYLHRLITHPSYNKLARDFERFVRRLPKGEI
                     AMKLAHKAEEGFKGAIADVALQPSPSGGEMDGRGGYGSSLIFEEMGVRYLGPIDGHNL
                     PLLINTLEFAKAYDRPVVIHALTQKGKGYAAALKNPEKFHGLGPYDVATGATPPAPPD
                     APPNWQDVFGQALVRLCQKDNSIVGITAAMPSGTGLKYLEQAMPNRYYDVGIAEEHAV
                     LFACGMATMGFHPVCAIYSTFLQRAYDCIVHDAALQDLPVIFCMDRAGLAAQDGPTHH
                     GLFDIAFVRPVPNCIAMAPKDEDELVDMLFTASHEPHPTFIRYPRGPAEGVPLKEQPR
                     LIEIGKAEVIRNFTNNGGLKVALFPLGNMMALGRQVATQLSAEGYDVAIINPRFIKPL
                     DAAVTRFFGEAADALVSMEDGILQGGYGSAILELFSEQRISTPVIRIGWPDQFIEHAS
                     TTEDLRTKYGLTVEGTVARVRAEFARASVQGHSAIAQLRQ"
     gene            7848..9104
                     /gene="icd"
                     /locus_tag="PKI07_11425"
     CDS             7848..9104
                     /gene="icd"
                     /locus_tag="PKI07_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOC51694.1"
                     /translation="MPYQNVVPPAGEKISVVNGKLNVPSNPILPFIRGDGTGPDIWAA
                     SQRVFDAAVRKAYNGQRRVAWFEVYAGEAARNKFDTWLPDDTIAAFKEYLVGIKGPLT
                     TPVGGGIRSLNVALRQMLDLYVCLRPVQYFPGVPSPVKHPEKVDMVIFRENTEDIYAG
                     IDYQAGSPEALRLLEFLQREMPAAFKKIRFGNEAMASVGIGVKPVSRPGTERLVRAAL
                     EYAVRHQRKSLTVVHKGNIMKYTEGAFRDWAYELARREFGAVELDGGPWCRTPQGLVI
                     KDAIADITLQQVLTRPEDFDVIATLNLNGDYLSDALAAQVGGIGIAPGGNLNYVSGHA
                     IFEATHGTAPKYANQDRVNPGSVVLSGEMMFRYLGWDEAANLIIQGLRGAIGSRRVTY
                     DFARLMDGAVEIKCSEFGDNIIAHMS"
     gene            complement(9101..9949)
                     /locus_tag="PKI07_11430"
     CDS             complement(9101..9949)
                     /locus_tag="PKI07_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC51695.1"
                     /translation="MKTVWITGAGGLIGSTLARTAPGAAHELKVVALTRDRLDLTDAA
                     AVRSEFRRQPPQLVIHCAALSKSPACEANPRQARNLNVGVTALLAELAAEIPLVFLST
                     DLVFDGRAGNYAEEAAVNPLSVYAETKAAAEQVLRANPRHLIVRTSLNGGRSPTGDRG
                     FNEQIRRAWQAGQTLSLFTDEFRSPISAEVTARAIWELVAQNATGVYHVAGSEKLSRW
                     QIGRLLAARWPQLQPRFEAASLKDHAGAPRAPDTSLNCAKAQARLSFRLPGLTEWLSS
                     HPGEVF"
     gene            complement(9946..10869)
                     /locus_tag="PKI07_11435"
     CDS             complement(9946..10869)
                     /locus_tag="PKI07_11435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC51696.1"
                     /translation="MALFTIQNEFRYEIVRKIYEGGMGIVYEAEQHGAREFVKRVAIK
                     VIRQNYANQKRFIENFIGEAKLVADLIHTNIAQTYHLGETNGVYFIAMELIRGVNLEQ
                     FSGQLSDKKRVLPKELAVFIVSRVARGLAYAHAKRDRDGKPLGLVHRDVSFKNIMIAM
                     EGDVKLTDFGIAKARGFLADNEGEVVAGKADYMSPEQADFQITDQRSDLFSAGVVLAH
                     LLLGKNIFKGATTEESRQRMIGLPIPDFRSLDPRIDDRLNEILHRALARERDQRYGTG
                     NELLYDLEHYIYHSGYGPTNETLGKYIRELY"
BASE COUNT         2164 a         3332 c         3711 g         2140 t
ORIGIN      
        1 atggacgagt cgcggatgat gtaaaaggac gtttcaaggc gggagggagt ggacgcgaac
       61 gttcggcaaa gccggaacgg gcggacgggt gttgtcgcgc accctggttt ttgggcggga
      121 tggagccacg gttggcgggg acattcccca acccgggcgg aggttgccga gaggcgactg
      181 cttgcgttgc ccccaagatc agtcggccgc tttgaagcca tagacgtttt catccccgcc
      241 ccctgagccg gaaaagcggc tttgggtttg ggaaaatctt ttccccctta tctaccgaaa
      301 ctcaggcgct gataaccaaa ggccctgggg tgctggaaga ggattggggt tgcggattgc
      361 gcgttgcggg gggcgcacag taagctggcg ccgtgaaact gttgttcatc gcggatatcg
      421 tagggcaacc gggccggcgc gcggttaagg agttgctgcc gcagttgcgg caagagcacg
      481 gggttggcgt ggtggtggcc aacggcgaga atgccgcggg cggttcgggc atcacgggga
      541 aaaccgctga ggaaatcttt gcggcggggg tggatatcat aacttgcggg gaccatcttt
      601 gggaccagaa ggaggtcatg gaactgcttg cgggtgagcc ccggttcgtg cgccctttga
      661 actacccgcc cggcacgccc ggtcagggca gcgcgatctt tgcggcgccc gggctgccgc
      721 cgctggcggt ccttaatctt cagggccgta cctttatgcc gccgttggaa aacccgttcc
      781 ttgccgcgcg ggccgaggtg gagcggcttc gccagcagac ccggctcatc ttcgtagatt
      841 ttcatgccga ggcgacgtcg gagaagatcg cgctggcgcg aatgctggat gggcaggtga
      901 gcgcggtgat cggcacccat acgcatgttc aaaccgccga cgagcaaatc ttccccggcg
      961 ggacggcgtt tctatctgac ggcggcttca ccggcccgca tgaaagcgtg ctggggcgcg
     1021 agatagaacc ggtcattcaa cggtttttaa ccagcatgcc gcagcggttc gaggtggcga
     1081 aggcgcgggt tcttttgcag ggggcggtgg ttgaaatcga ggagcaaacc gggcgcgccc
     1141 aaagcatcca acgcgtatcc tgttcgttat gaggccgaga tggaatgccg ccttgtttcg
     1201 gccgggaacg cccgcccgcc ggccggcgtt gaataaggat cagccacggg aaaacccgct
     1261 gaccatcgga ccgttggcgg gtggctgctg ccgcggcggg gctgccgcgt gttaacaatg
     1321 gcccggatct ccaaatccca gtgggagttt gacgggctgt ttccgtcgga gcccgcccgc
     1381 aaggtgctga ccgtcagcga actgacgtgg caggtccggc gtttgctgga gcagcaagtt
     1441 ggggaggtct gggtgacggg cgagatcacg aaccggcggt tgcaaagttc ggggcatatc
     1501 tacttcacgc ttaaggacgc cggcgcacag ttgagttgtg tgctgtttcg gggggagacg
     1561 caggcggatc gcgcgctggt gcaggatggc cggaaagtca acctgcgggg tgaggtcacg
     1621 gtttatgagc cgcgcgggca gtaccagttg cgggtgatga aagtcgagtt gcagggcgtt
     1681 ggggcgctgc aggcggcttt tgagaaactc aagcagaagc tcaaagccga ggggttgttt
     1741 gcgccggagc ggaagcgtcc gctgccgcgg tatcccctgc gcattggggt ggccacctcg
     1801 cccaccggcg ccgccctgcg cgatgtgctg cacgtggtcg agcggcgtca tccggcgctg
     1861 gagctcattc tcgcgccgtg ccgtgtgcag ggggagggtg cggcggcgga aatcgccgcg
     1921 gccgtgcgcc tgctgaatga gtttaatcgc gcggcggcgg cggcgggcga tcgttcggga
     1981 ggggtggatt tgatcctcgt tacccggggg ggcggcagcc tggaagactt gtgggccttc
     2041 aacgaggaag tggtggcgcg ggcgattttc gagtcggccc tgccggtcgt ttcggcggtg
     2101 ggacatgaaa tagatttcag cattagcgat ttcgtggccg atgtgcgcgc ggcaacgccc
     2161 agcgcggcgg cggagatcat taccgagggc gtctttgcca gcgggcagtt tttgtcggaa
     2221 gctccccgcc gcctccgcca gtcggcggcg gaacagtggg aagacaagcg gcgcgactgg
     2281 gccgaagccg cgcagcgcct ggcccggatg catccgcgcc ggcggttgaa tgattggctg
     2341 cagcggctgg atgacgcgca aacgggcctg ctgcgctgcc tccggcaggg catgcggcaa
     2401 cagcggcaag gctggcacaa cctggcggaa cggctgtggc gggtccgccc ggcggcgctt
     2461 tggcggcaga aacgggatgg ggttcaattg gcggaacagc gtttgcgcga gcaggggcgc
     2521 cggcttctgg aagcccggcg ggcgcagtta gacgccctgg ccgcccggtt gcggctgctg
     2581 gggccggagc aagtgctggc gcgcggctat tcgatcacct cggacgcggc gagcggcaaa
     2641 gtcctgcgcg cggcggacga ggtcaagccc ggccagcgtt tgcgaacccg gctgaaaacg
     2701 ggcgaaattc ttagccaggc cgagggttga tcagggatac cgcacgcgga agaacatctg
     2761 cggggcggcg acggaattag tgaaagacac gtccgttccg ggcgaggaag cggtgcccac
     2821 catcacccag ttggactgcg caaggttggt cttcgcctgg acgacgtagg gcaggttgct
     2881 gagcccctgc caggcgacga cctggggcga ccgcatcacg aggaccggcg ccggaaccac
     2941 cagctcgagt tgcgcgtccg ggctgatgac cgtgccggcg gaattgctga ctaccactga
     3001 atacgccccc tggtcggccg gttggacatg gttgcacgta tagacgctgg cggtcgcccc
     3061 ggaaaggggt gtggcattaa agcgccactg gtagctgagg ggggcggtgc cggtggccag
     3121 cacgctaaag gtggcatctg cgccctgctg gacccgttgg ctgacgggag gcgcggtgat
     3181 ggtgggcggc acgctcaccg ggatggggag accggtaacg gtgaccatat caaagcggac
     3241 cgtgccgccg ggggcatagg tggagccgct cttggcggcc acgtagttat tgccgcccgc
     3301 gcccgtggcg gtgctctcaa actcggcgac aatctggaaa gcaaagtccg gattattgtt
     3361 tgcgcccgca agggttccga gactgttggt cttggattcc caatcatccc tggcgactga
     3421 tatgcccgtg ggaaaatcaa tgaaatcggc accgttggtg gtatagcgaa ggcgcatgta
     3481 tttgctgccg gtgctgcttg gccgttggtc ccaggcaatg ctgatatctt gccagccgac
     3541 ggtgctgacc gtgaagagcg tgcccgccgt cttattgccg atggtggccg ccgggtagga
     3601 tgaggtgttc caggcgctgt tgtcggacgt ttgggggtcc tttgcgctgc ccttggcaaa
     3661 ttcgccggag cccggcgcct ttgtgccgcc aatgtaggag gcggtgccgc tgcctatcga
     3721 gggggtcaga gtgccggtag tcgtctggcc atcgggcgtc gggctgttga agttccactg
     3781 ggcaataata tggcccgaag ccaccgcaac cgtcaggagg gcctccgtgc tgacaaccga
     3841 tccggccagg ttggtaatca cgaccgaata attgccggca tcgccaggct ggacattggc
     3901 gcgtgtgtaa ctggatccgg tcgccccggg gagcggcaga ccgttgaagc gccactgata
     3961 gaagagtggc atcgtcccgg tcgcggtgac ggtaaaaacg gccgattggc ccggggccac
     4021 ggtctggctt tcgggttggg cgacgatgac cggggcggcg gcaatcgtca gggaggccgg
     4081 cgcactggtg acgctgccgg caaagttggt gatgaccacg ctgtaatcgc cggcctggcc
     4141 ggattgcaca ttcgtgagcg ccagcatgct ggtggttgcg ccggcgaggt tggcggcgcc
     4201 aaaccgccac tggtaatgca gcgcgccggc gccagaggcg gctacgctaa aggccacatc
     4261 gctgccgggc gcggcgatct ggctcgcggg ttgggtgatg atgaacggcg cgtccggcca
     4321 ggcattggtg atggtgtaag tgatgttgaa atctttgacc acggccatgt gctgcatgcc
     4381 gcttacgtgc gaatcccccg gggcgacggg ggcgacatcg ctcaaagggg tatagacggc
     4441 ggaatcgaac accagcccat tggaaaacac gcgggagggg aggacggtgg gcacgaaatt
     4501 ggtggcgaag gagtggctgg gcaagacgta atcgtagggt ttattgcgcg gctcgttggt
     4561 ctccattttg ccccctgatt cggcgtcagt ggggatggga ttatcactga ggaaggtctt
     4621 aaaggttgac acggccgcct ccgagcgagt ggcggtgttg aaatcgcccg cgacgacgac
     4681 ccaggcgttg gcggggaagt tcgcgctgat aagatttttg agcgcctggg cctcctggtt
     4741 gcgatcgctc gcgctgccac tgctgtatag atgcacactg accacataca aatcattgga
     4801 gccgggcaca tccaaccggg cccaggcgaa cccgcggtcg ggcaccaggg aatcatccca
     4861 ggaaccggcg gcccggatgg gaaaacggct gatgatgccg ttggggatgc tgtagcccga
     4921 ttcgcgatag tacacgaagt tagtcccgaa agtcgtatcc agcatgtggc ggaaatccgc
     4981 cgcgctattg ttgccgtagt tgaattcctg aatggccaca atgtccggtt tgagcccctt
     5041 gagaatattg aggccggcgg tctggtaagt tggccctccc gaagtcaggt tggcggccat
     5101 gatccgcagc gtcaggttgg tttgaaaaac cccgggcgtt tgcgcgccaa gagaacctgc
     5161 cagcgccaac accccgaatc ccagacattg gccaacacga ataataagct tcataaacga
     5221 caagcggaca ctggttctat ggcgctataa ctaacggctg gacgttaaaa atcaagctgg
     5281 gaaaccgaaa tacggcgccg ggctcgccgg caggcaacgg cttctcgctc cccccatttc
     5341 acataaactc aatactggac gggcggatgg ggttgtccta gtctcccgtt atgccgagtg
     5401 tttcagaaaa tgcgggccaa agcagcgccg ctaaggcggc ccaattgccc tttgaagaag
     5461 ctttgcgaaa gctggaagct ctggtcgtcg cgatggaatc agaggatctg tcgctggaag
     5521 ccttgctggt caaatacgaa gagggcacgc gcctggtcaa agcgtgccag gacaagctga
     5581 cgaaagccga ggcgcgaatc cggcaattgg aaaagacggc ggcgggggaa atgaaacttc
     5641 agcccctggc attgcccgag gacgcgcgtg aataagacaa cgccggcgtg gagtaagcgt
     5701 gtatgacggc ggcttttgtg ataatcccga accccgaccc cccgacatga gccgatatct
     5761 ggagatggtg gacagtccgg cgcacgtcaa gaaactgacg ctggagcaac tgactcaact
     5821 ggcggaggag attcggcagg aactcattac cgtgctggcc aagcatggcg ggcacttggg
     5881 gcccaatctc ggggtgatcg aattgaccct ggccctgcac tacgttttca acacgcccag
     5941 ggacaaattt atctgggacg tgagccacca ggtttacgtg cataagctgc tgaccggtcg
     6001 caagcatcgc tttgacagca tccgcagcac gggggggctg agcgggtttg ccgtgcgcgc
     6061 ggagagcgag catgattgct acggcgcggc gcatgccggc acggctttat ccgccgccct
     6121 gggcatctgc gccgggcgcg atcagcgtgg cagcgatgag cacgtcgtgg cgatatttgg
     6181 cgatggcgcg ttgaccaacg gcatttcctt cgaggcgctg aacaacatcg cgccgaccac
     6241 caaacggttc attggcatcc tgaacgacaa tgaatggggc attgccaaaa acgtcggggc
     6301 catcgccaac tacctccacc ggttgatcac ccatccttcc tataacaagc tggcgcggga
     6361 tttcgagcgt ttcgtccggc gcctgcccaa aggcgagatt gcgatgaaac tcgcgcacaa
     6421 agctgaggaa ggtttcaaag gcgccatcgc ggatgtagcg ctccaaccca gcccgagcgg
     6481 aggggaaatg gatggccgtg gcggctatgg cagcagcctg atcttcgagg agatgggggt
     6541 gcggtacctg gggccgatag acggccataa cctgcccttg ctgattaaca cgctggagtt
     6601 tgccaaggcg tatgatcggc ccgtcgtaat ccatgctttg acccaaaaag gcaaaggcta
     6661 cgctgccgca ctcaagaatc ccgagaagtt tcatggcctg ggcccctacg atgtcgccac
     6721 gggggcgact ccacccgccc cgcccgacgc gcccccgaat tggcaggatg tcttcggcca
     6781 ggccctggtg cggctttgcc aaaaggataa ttccattgtc ggcatcaccg cggccatgcc
     6841 cagcggcacc ggcttgaagt atttggagca ggcgatgcct aaccgctact acgatgtggg
     6901 catcgccgag gagcacgccg tcttgttcgc ctgcggcatg gccaccatgg gctttcaccc
     6961 cgtctgtgcg atttattcga cttttcttca gcgcgcgtat gactgcatcg tgcatgatgc
     7021 ggcgctgcag gacttgccgg tgatcttttg catggaccgc gcgggactcg cggcgcagga
     7081 cgggccgacg catcatgggt tgttcgacat tgccttcgtt cggccagtgc ccaactgcat
     7141 tgccatggcg cccaaggatg aagatgaact ggtggacatg ctgtttacag cctcccatga
     7201 gccgcacccg actttcattc gttatccccg cggcccggcc gagggcgtcc cgctcaagga
     7261 acagccccgg ctgattgaaa tcggcaaagc cgaggtcatc cgcaatttca ccaataacgg
     7321 cgggctcaaa gtggccctgt ttcccctggg caacatgatg gcgctgggac ggcaggtggc
     7381 cacccagttg tctgcggaag gatatgatgt cgccattatt aacccccggt tcatcaaacc
     7441 attagacgcg gcagtgacga ggttctttgg cgaggccgcc gacgcgttgg tctccatgga
     7501 agacggcatt ttgcagggcg gttatggcag cgccatcctg gagttgttca gcgagcagcg
     7561 gatcagcacc cccgtcatcc ggatcggttg gcccgatcag ttcattgaac acgcctcgac
     7621 caccgaagat ctgcgcacca aatacggcct gaccgtggag ggcacagtcg cccgggtgcg
     7681 agcggagttt gcccgggcat cggttcaggg tcattccgcc atcgcccagc ttcggcagtg
     7741 acacccttga gcggccaatt actgactgcg ccccggcatt ccgcgcgtga tttttcctgc
     7801 cgcaccgtga attctactca ccccgtaaag accttgagct aaccactatg ccttaccaaa
     7861 atgttgtccc gccagccggc gaaaagattt ccgtcgtcaa cggcaaactg aacgtgccgt
     7921 ccaacccgat actgccgttc atccgcggcg acggcacggg ccccgatatt tgggccgcca
     7981 gccagcgcgt gtttgacgcg gcggtccgga aggcctacaa cggccagcgc cgggttgcct
     8041 ggttcgaggt gtatgccggc gaagctgcca ggaacaagtt tgatacctgg ttgcccgatg
     8101 acacgatcgc ggccttcaag gaatacctgg tcggcatcaa aggcccgctc acgacccccg
     8161 tcggtggcgg catccgctcc ctcaacgtcg cgctgcgcca gatgctcgac ctgtatgtgt
     8221 gcttgcgccc ggtgcaatat ttcccgggag tcccctcgcc ggtgaaacac ccggagaagg
     8281 tggacatggt catcttccgc gaaaacacgg aagacatcta tgccggcatt gattaccagg
     8341 cgggcagccc cgaagcgctg cgattgctgg agttcctcca gcgggaaatg cccgcggcat
     8401 ttaagaaaat tcgatttggc aacgaggcga tggcatccgt gggcattggg gtcaagcccg
     8461 tgagccggcc gggcaccgag cgcctggtgc gcgccgcgct cgaatacgcc gtgcggcatc
     8521 agcgcaagag cctcacggtt gtccacaagg gcaacattat gaaatacacg gaaggcgcat
     8581 tccgtgactg ggcctatgag ttggcccggc gtgagttcgg cgcggtggag ttggacggcg
     8641 gcccgtggtg ccggacgccc cagggcctcg tcatcaagga tgccatcgct gatattaccc
     8701 ttcagcaagt gctgactcgc ccggaggact ttgatgtcat tgccacgctc aacctcaacg
     8761 gcgattacct gagtgacgcg ctcgcggcgc aagtgggcgg cataggcatc gcgcccggcg
     8821 gcaacctgaa ctacgtttcc gggcacgcga tcttcgaggc cacgcacggc accgcgccca
     8881 aatacgcaaa tcaggaccgg gtcaaccccg gcagcgtcgt gctcagcggg gaaatgatgt
     8941 ttcgctacct cggctgggac gaggccgcca acctaatcat tcagggtctc cgcggtgcca
     9001 taggctcacg gcgggtcacc tatgattttg cccgcctgat ggatggagcg gtcgaaatca
     9061 aatgttccga gttcggggac aacatcatcg cgcatatgag ctaaaagact tcgccgggat
     9121 ggctggaaag ccactcggtc agtccgggca ggcgaaaaga cagccgcgcc tgcgctttgg
     9181 cgcaattcaa tgaggtgtcc ggggctcgtg gggcccccgc gtgatccttt agcgaggccg
     9241 cttcaaatcg gggctgcaac tgcggccagc gtgccgcaag gaggcggcca atctgccacc
     9301 gggacagctt ctcgctcccg gcgacgtggt aaaccccggt tgcgttctgc gcgaccaatt
     9361 cccaaatagc gcgcgccgtt acctcggcgc tgatgggaga gcggaactcg tccgtgaaca
     9421 agctcagcgt ttgcccggcc tgccaggcgc ggcgtatttg ctcgttgaat ccacggtcgc
     9481 cggtgggcga tctgccgccg ttgagcgaag tgcgcacaat caaatgccgc gggttggcgc
     9541 gcagcacctg ctccgccgct gccttggtct cggcatagac gctgagagga ttgacggcgg
     9601 cctcctcggc gtagttgccg gcgcgcccat cgaaaaccag gtctgtggac aggaaaacca
     9661 gcgggatttc agccgccagt tcggccagca aggccgtaac cccgacgttg aggttgcgcg
     9721 cctgcctcgg gttggcttcg cacgcggggc tcttgctgag cgcggcgcag tgaatgacca
     9781 gttgcggtgg ttgacggcgg aactcgctcc gcaccgccgc ggcatcggtc agatccaacc
     9841 ggtcgcgggt gagggccacc acctttagtt catgcgcggc gcccggcgct gttcgggcca
     9901 gggtgcttcc aatcagcccg cctgcgccgg tgatccagac cgttttcaat acaactcgcg
     9961 gatatacttg cccagcgtct cgttggtcgg cccataaccc gagtggtaga tgtagtgctc
    10021 cagatcgtag agcaactcat tgccggtccc ataccgctgg tcccgctcgc gtgccagggc
    10081 gcgatgcaga atctcgttca gccggtcgtc aatccgcgga tccaacgacc ggaaatcagg
    10141 aattggcaac ccaatcatgc gctggcggga ttcctccgtg gtggcgcctt tgaagatgtt
    10201 tttgcccagc aacaagtgcg ccagcaccac cccggccgaa aacaagtccg accgttggtc
    10261 ggtgatttgg aaatccgcct gctcggggct catgtagtcc gccttgcccg cgaccacctc
    10321 cccctcgttg tccgccagaa atccgcgcgc tttggcaatc ccgaaatcgg tcagcttcac
    10381 atccccttcc atggcgatca tgatgttctt gaaactcaca tcccggtgca ccaaccccag
    10441 cggcttgccg tcccggtccc gtttggcgtg cgcataagcc agcccgcgcg ccacgcggct
    10501 gacaatgaag accgccagtt ccttgggcag aacgcgcttc ttgtcgctca attgcccgga
    10561 aaattgctcc aggttcaccc cccggatcaa ctccatcgcg ataaaataca cgccgttggt
    10621 ttcgcccagg tgataggtct gcgcaatgtt ggtatggatt aaatcggcca ccagtttcgc
    10681 ctccccgatg aagttctcga tgaaccgctt ctggttggca tagttttggc gaattacctt
    10741 gatggccact cgcttgacga attcccgcgc gccgtgctgc tcggcctcat agacgatccc
    10801 cattccgccc tcataaatct tgcgcacgat ttcgtaccga aactcatttt gtatggtgaa
    10861 aagcgccaca cggcgatcct gattgaaaac tgtcaggagt caagtctggc attcacccgg
    10921 gcgcgcaagc gagttgcctc cggataaatg ttgccggggc ggaaaggcgg acaaggcgtt
    10981 cgccgatacg ttgactcaag ttcagggttg ccgaagcacc gcacatgagc ccatcctcca
    11041 aacgggaatc ccgggcccgc attcacggcc gctaccagcg cgccgtcggc tgtagaagcg
    11101 gcgcatttgg gatgagggtt gcgccacccg cggtgatcac cgtaaaagcg ccctgcgcct
    11161 gtggcaccgg ccgctttgaa tcaacagacg ctttcatccc aatcccctca acccgaaaaa
    11221 ctgctccggg tttggaaaca tcttttccct cttatctaca aaaacttagg cgatcacaaa
    11281 gagccccgcc caccaccacc aatcccaaaa aattgccctt gcgcgaagac ctcccgcccg
    11341 ccagcct
//
LOCUS       DAOPQG010000112        11255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6635_length_11255_cov_15.298839, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000112 DAOPQG010000000
VERSION     DAOPQG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6635_length_11255_cov_15.298839"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            603..1328
                     /locus_tag="PKI07_11440"
     CDS             603..1328
                     /locus_tag="PKI07_11440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOC51697.1"
                     /translation="MSELFEIIYEDAGLLVVNKPAGLVCHPTKTDQWSSLIGRARLYL
                     GQGAQPHLVNRLDRETSGVTLLAKEPEAARALRRLWEGRQVEKEYLAVVHGHPPAPHG
                     VIDAPLGKDERSPVAIKDCVRADGAAAETEYWVRQRFTREQTPFALLRVLPRTGRKHQ
                     IRIHLAHLGHPIVGDKIYGGDEGLYLALVEGRLTAEQRRRLILPHHALHAREVRLAWR
                     GQATVFRAEPEPWLAEWLAGAGG"
     gene            1368..4067
                     /locus_tag="PKI07_11445"
     CDS             1368..4067
                     /locus_tag="PKI07_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51698.1"
                     /translation="MSDNDRQSSDRIPEPFPFGPAPAADSRPGRLWGWAAFLVAATLA
                     AYLPVGGAGFIWDDDTFLWRNPLILGADGLFRLWFTTEAPDYFPLTSTTLWLEWRLWG
                     ANPLGYHVVNVLLHAASAVLLWRVLARLKIPGAFLAAAIFAVHPVNVASVAWITERKN
                     TLAMVLYLASLWFYLRAEEERELRPPAGEAAAAAPGFSRFDALSLLAFLGALLSKTAV
                     VPLPVVLLGLAWWRRGRLELRDLGRSLPYFALALAAGVVTVWFQYHRAMGASQLDVRA
                     DSLGSRLAGAGWAFWFYLYKALAPLRLSFVYPRWQINPARVASYVPGLLILGLLGIGW
                     RYRRGWGRPGLLGLGYFLVMLLPVLGFLNIYFMRYSLVANHWQYFALIGPIALFAAGF
                     TRAGRSMSAAAGRAGWPRVSAFGFSLSAFGLVLALGVLTGREAATYKNLKTLWTTTLA
                     RNPAAGLAHNNLGTLLLEEGRPAEAEAHFRQALALQPKAADVSSNLGGALLAQGRFAE
                     AIPPLQQAIKLQPDSAQAHNNLGSALIRQGRMDEALAHFRRAVELRPGLASARGNLGG
                     ALLQAGQADAAIAELQRALELEPDLADAQVSLGNAWLAKGRGADAIACLEKAVRLQPR
                     LAAGHYHLANALLQQGRVDEAIARFQQALSLQPGFAPAHNGLASALLQKGRADEALAH
                     LRAALQIEPRLAEAHLNLANLLLQRKQADEAITHFRAALEIQPGLPAAHNNLASALLA
                     RGRVDEAILHCRRALELAPGLAEAHNNLANALLQKGRCGEAAAHYQAAVAARPDHPYL
                     LNNLAWVLATCPEAAARNGARAVELAEQARRRAGDADPMLLGTLAAAYAEAGRFADAV
                     ATAQRALEQASAQANPESVAALRAQLERYRSGLPFRDAPPPAR"
     gene            4183..4740
                     /locus_tag="PKI07_11450"
     CDS             4183..4740
                     /locus_tag="PKI07_11450"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOC51699.1"
                     /translation="MRILVAITGASGSLYAQRLLENLAPAGHELHVVLSQYAPAVIAE
                     ELPGGLRLPPGAITHDLQRLSVPFASGSNAPDAMVVIPCSMGTLGRIAHGYSENLLLR
                     AADVVLKERKQLILVPRETPLSLVHVRNFELLLQAGATILPANPSFYSRPRSVAQVAD
                     TVVARVLDHLGVAHKLVARWQEERE"
     gene            4799..5695
                     /locus_tag="PKI07_11455"
     CDS             4799..5695
                     /locus_tag="PKI07_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOC51700.1"
                     /translation="MFATLRKWASFVTFSHTVFALPFALAAMAVAARDHRGWPGWRVF
                     GLILAAMVCARTCAMAFNRIVDRKFDALNPRTARRHLPAGHITLASAATLCALSAAGL
                     VGASYLLNPICFYLAPVALVVICFYSLTKRFTDYTHVFLGLSLALAPVGAWLAVKGLE
                     ISRWEILQMLVLAAVVVLWLVGFDIIYALQDYEFDRAQGLHSLVVAWGPANALRAAFL
                     AHVLMCGLLFVFGLLCRFRIAYIIGWVLIVGCLVLEHWIARRRSLNWINLAFFRLNAV
                     VSAIFLVVTLAEVIFQGGFRLR"
     gene            complement(5829..6818)
                     /gene="pdxA"
                     /locus_tag="PKI07_11460"
     CDS             complement(5829..6818)
                     /gene="pdxA"
                     /locus_tag="PKI07_11460"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOC51701.1"
                     /translation="MTARIGISLGDITGIGPEVTLKALALEAPADDTRYLLIGDAEHT
                     RRLNAQLGLNLPLQADAGGDAPGRFCLCHPLPDALPAGLPAGAPAAARAAYAWVREGA
                     ERCQRRQLDALVTAPVSKEAIVRAGQPFVGQTEMLSQLAHAQRTAMMLLGHDDRGRWL
                     RVALATTHLPIKRVSDHLTQAKIEQVIELAAEACRDLGLPRARVAVCGLNPHAGEGGE
                     MGTEEQTIITPAVRAAQSRQLDVAGPLAADALFYHAFKGEFDAVVAMYHDQGLAPLKM
                     IAFETGVNWTLGLPFIRTSPDHGTAYDIAGQGKANPSSMVAALRLARQLARNR"
     gene            complement(6885..8030)
                     /locus_tag="PKI07_11465"
     CDS             complement(6885..8030)
                     /locus_tag="PKI07_11465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC51702.1"
                     /translation="MRTLSIMLVAAAGFSFTPRASAETHLINAIQAVVHDSVVTFAEL
                     QDRMTPARELLVRQYRGQPELLEQKVTAALNENLEQLIERQLILQDFRSKFAQKERLE
                     HANKIIGKDIDKDIEREIHTRYGGDRVAFMRTLQAEGVTLERYRQQIRNRIIISWLRQ
                     ENISSSIIISPHKVEEHYLARREEFKVQEEVKLRMIVLKCPGQTEAASTAALAEDILT
                     KLKEGATFAEMASIHSEGSQKTEGGDLGWWELSRLSRGLADTAAALQVGQNSSVLSRS
                     AGDDYWVCQYSNKAPVLARHYVVDLALKKEDLVEERRLESAAAAANLPPPVEFYLMQV
                     EDKRPERFKTLAEVREQIEQDLLVKEQTRLANLWLDRLKKKTFVQVF"
     gene            8427..9578
                     /locus_tag="PKI07_11470"
     CDS             8427..9578
                     /locus_tag="PKI07_11470"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOC51703.1"
                     /translation="MKTNQWLGILAAALSGVLLAGCQSTPSPTPAKGQISHRPFGRTP
                     DGTAVDLFTLRNANGVEAGICNYGGLVIFLKVPDRHGRLGDVTLGYDKLEDYLKDSPY
                     FGALIGRYGNRIAKGKFTLNGQEYTLAVNNGPNALHGGLKGFDKVVWQPTIRATREGP
                     ELELNYVSRDGEEGYPGTLRVTTIYSLTPDNALKIQYRATTDKDTVVNLTHHSYFNLA
                     GQGDILNHQVMLPADTFTPVDRTLIPTGELRPVAGTPFDFRTPTAIGARIGQDDEQLK
                     FGGGYDHNWVINKPLGQLGLMARVYDPASGRVMEVLSTEPGLQFYSGNFLDGKNIGKG
                     GWAYKFRNGFCMEPQHYPNSPNQPNFPSVVLKPGQVYHNTILYRFSVQK"
     gene            9676..10794
                     /locus_tag="PKI07_11475"
     CDS             9676..10794
                     /locus_tag="PKI07_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51704.1"
                     /translation="MQTFPDPLDLASLKVRPLAERESRSRLEHILVDPGAPPRACAPE
                     ALAAVHAAAQRLAAARQRQASVILMYGAHLVKNGALAIVNRLLTDGWITHLATNGAGT
                     IHDWELSFLGRTEESVRQNVATGSFGAWDETGRCQHLALLAGALRGEGYGRSLGRFIS
                     EDGVTLPDPAALAQSLRERPDDPLAPARADLLQAMRAHQLPAGRILVPHRWKETSILG
                     QAFRQNVPLTVHPGLGYDIIAVHPMFNGAAIGRAAAVDFRLFSRAVEGLDGGVVLSIG
                     SAVMAPQVFEKSLSCVNNLRLQAGRPAVRDLEFYVVDLQDGGQWDWSRGEPPRSSPAY
                     YLRFCKSFARMGGRMNYVQCDNVAFLHNLLHHLPAPRR"
     gene            10901..>11255
                     /locus_tag="PKI07_11480"
     CDS             10901..>11255
                     /locus_tag="PKI07_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOC51705.1"
                     /translation="MFDSLSSKLEKVFRDLRGLGKISEANASAALREVRQALLEADVN
                     FKVARDFIERVRAQSLGQEVIQSIQPGQQVIKIIHDELVQLLGATNAGLQLSGNPSCV
                     LMVGLHGSGKTTSSGK"
BASE COUNT         1726 a         3694 c         3821 g         2014 t
ORIGIN      
        1 gtggggcggg tttgggcgcg ggcctgagcc ggaagggagg aatggacgac ttgcggacga
       61 tgtaaaacga cgttccaagg cgggagggag tggacgagaa cgttcggtat gtccggaacg
      121 gacggccgag tgttgtcgcg caccgtggtt tttgggcggg atggagccac ggttggcggg
      181 gacattcccc ctcccgggcg gaggttgtcg caaggcggct gcttgcgttg ccctcatcgc
      241 gggcggcgta ttcgcccgat tggggtgagt ttggttggag atttatcccc agactttgcg
      301 cgcagggtga gtgatggacg ggattggttc gggaatctgt tcaggagcgt tggttgtgta
      361 gatcttgagc tggagcggtt gaggctgcgg gttgggtggc ggttaagcgc ttcaaagaag
      421 gtgtcttgca gcgcgacaaa ggccttgggg tccatgcgcg tgaacgcctg ataaaacacg
      481 gcacagcccg gccccttgta aaagcgacga tttggccttc ccggtgcgag ttgaaaagcc
      541 ccggcgaaag aagggttgct ccgggaacgg agcccggcat gatttcgcct ggccattcca
      601 ctgtgtcaga acttttcgag atcatttacg aggatgccgg gctgttggtg gtcaataagc
      661 ccgccgggct ggtatgccat ccgaccaaga cggaccaatg gtcgagcctg atcggccgcg
      721 cgcggttgta tttggggcaa ggcgcgcaac cgcacctggt caaccggctg gaccgggaga
      781 ccagcggcgt gacgttgctg gccaaggagc cggaggcggc gcgcgcgctg cggcggcttt
      841 gggaggggcg gcaggtcgag aaggagtacc tggccgtcgt gcatgggcat ccgcccgcgc
      901 cgcacggggt tattgatgcg ccgctgggca aggatgagcg cagcccggtg gccattaagg
      961 attgcgtgcg cgcggatggc gcggcggcgg agacagagta ctgggtgcgg caacggttta
     1021 cgcgggagca aacgcccttt gccctgctgc gggtgctgcc gcgcacgggg cgcaagcacc
     1081 agattcggat tcacctggcg catttggggc atcccattgt gggcgataaa atctacggcg
     1141 gcgacgaggg cctgtacctg gcgctggtgg agggccggtt gacggcggag cagcggcggc
     1201 ggttgattct gccgcaccat gcgttgcacg cgcgcgaagt acggctggcc tggcgcggcc
     1261 aggcaacggt cttccgcgcc gagccggagc cgtggctggc ggagtggctg gcgggcgccg
     1321 gcggctaatg attgcgcaac tggcgggatt gcttcaggct tttggccgtg tccgataatg
     1381 accgccagtc cagcgaccgc atcccggagc cgttcccgtt cgggccggcg ccggcggcgg
     1441 attcccgtcc ggggcggctc tgggggtggg ccgcgttttt ggtggcggcc actttggcgg
     1501 cttacctgcc cgtcgggggg gcggggttca tttgggacga cgatactttc ctgtggcgca
     1561 atccgctgat cctgggggcc gacgggctgt ttcgcctgtg gttcacgacg gaggcgccgg
     1621 attattttcc gctgacctca acaaccttgt ggctggaatg gcggctgtgg ggggcgaacc
     1681 cgttgggcta ccacgtggtg aatgtgctgt tgcacgcggc gagcgcggtg ctgctgtggc
     1741 gggtgctggc gcggctgaaa atccccggag cctttctggc cgcggcgatc tttgccgtgc
     1801 atccggtcaa cgtcgcgtcc gtcgcctgga taaccgagcg caagaacacc ctggcgatgg
     1861 tgctgtacct ggccagcctg tggttttatt tgcgcgcgga agaagaacgg gaactccggc
     1921 cgcccgcggg cgaagccgcc gccgccgcgc cggggttttc gcgcttcgac gccctgtcgc
     1981 tgctggcgtt cctcggcgcg ctcttgagca agaccgcggt ggtgccgctg ccggtggtgt
     2041 tgctggggct ggcgtggtgg cggcgcgggc ggctggagct gcgcgacctc gggcggagcc
     2101 tgccttattt tgccctggcg ctggcggcgg gcgtcgtcac cgtgtggttc caataccacc
     2161 gggcgatggg ggcgagccag ctcgatgtgc gcgcggattc gctagggtcg cggctggcgg
     2221 gggcgggctg ggccttctgg ttttatctct acaaggcgtt ggcgccgctg cgcctgagct
     2281 ttgtttatcc gcgctggcag attaatccgg cgcgggtggc gtcgtatgtg ccggggctgc
     2341 tgattctggg gttgctgggg attggctggc gttatcggcg tggatgggga aggcccgggc
     2401 tgttggggct gggctatttt ctggtcatgc tgctgccggt tttgggcttc ctgaacattt
     2461 atttcatgcg ctactcgctg gtcgccaatc attggcaata cttcgccctg attggcccca
     2521 tcgcgctgtt tgccgccggg ttcacccgcg ccgggcggag catgagcgcc gcggccgggc
     2581 gcgcgggttg gccgcgggtt tcggcgttcg ggttttccct ttccgcgttc ggcctggtcc
     2641 tggcgctggg cgtcttaacc gggcgcgagg ccgccactta caagaacctg aagacgctct
     2701 ggacgacgac gctggcgcgg aatcccgccg ccggcctggc ccataacaac ctgggcacgc
     2761 tgctgcttga agaggggcgc cccgcggaag cggaggcgca tttccggcaa gcccttgccc
     2821 tccagcccaa ggccgccgat gtgtccagca acctgggcgg cgcgttgctc gcgcaggggc
     2881 gtttcgcgga ggccattccc cctttgcagc aggccatcaa gctgcaaccc gattcggcgc
     2941 aagcgcataa caacctcggc agcgccctca tccggcaagg gcggatggac gaggcgctgg
     3001 cgcattttcg ccgggcggtc gaactccggc ccggcctggc cagcgcgcgc ggcaacctgg
     3061 gcggcgcgct gcttcaggcg gggcaggcgg atgcggccat cgcggaattg cagcgcgcgc
     3121 tcgaacttga gcccgatctg gccgacgcgc aagtgagcct cggcaacgcc tggctcgcca
     3181 aaggccgggg cgcggacgcc atcgcctgtc tggaaaaggc cgtccggctg caaccccgcc
     3241 tggcggccgg ccattaccat ctcgccaacg ccctgctcca gcaggggcgg gtggacgagg
     3301 cgattgcgcg cttccagcag gcgctgtcct tgcagccggg ctttgccccc gctcataacg
     3361 ggctggccag tgccttgctg cagaaaggcc gggcggatga ggcgctcgcc catctccgcg
     3421 ccgccctcca aatcgagccc cggctcgccg aggcccatct caacctcgcc aacctgctgc
     3481 tccagcgaaa gcaggctgat gaggccatca cgcatttccg cgcggcgctg gaaatccagc
     3541 ccggccttcc cgccgcccat aacaaccttg ccagcgcctt gctggcccgg gggcgggtgg
     3601 atgaggccat tctccattgc cggcgcgcgc tggaactcgc gcccggcctg gccgaagccc
     3661 acaacaacct cgccaacgcc ctgctgcaaa agggccgctg cggggaagcg gcggcgcatt
     3721 accaggccgc cgttgccgcc cggcccgatc atccgtacct cctgaacaac ctggcctggg
     3781 tgctggcgac ctgccccgaa gccgcagccc gcaacggcgc ccgggcggtg gaactggccg
     3841 agcaggcccg gcggcgggcg ggggacgccg accccatgct gctcggaacg ctggcggcgg
     3901 cctacgccga ggcggggcgg tttgcggacg ccgtcgccac ggctcagcgg gcgctggagc
     3961 aggcttccgc ccaggccaat ccggaatcgg tggcggctct ccgcgcgcaa ctcgagcgct
     4021 atcgctccgg gttgcctttc cgcgacgcgc cgccgccggc ccgctgaggg gcgcgccccg
     4081 aagcggcgca aatcgcaacg ctttcgccgg gggaaacccg cttgcccaat ccgcgccgcg
     4141 cggccatatt cgggccggct gccgttcaac tcttcgacgc ccttgagaat actagtcgcc
     4201 attacgggtg ccagcgggtc gctttacgct cagcgcctgc tggaaaacct cgcgcccgcc
     4261 gggcacgagc tgcacgtcgt gctcagccag tatgcgccgg cggtgattgc ggaggaactg
     4321 cccggcggtt tgcggctgcc gcccggcgcc atcacccacg acttgcagcg cctgtcggtt
     4381 ccgtttgcca gcggctccaa tgcgccggac gcgatggtcg tcatcccgtg cagcatgggc
     4441 accctgggcc gcatcgcgca tggctacagc gagaacctgc tcctgcgcgc cgctgatgtg
     4501 gtgttgaagg aacgaaagca gctgatcctg gttccccgcg aaacgccatt gagcctggtt
     4561 cacgtccgga acttcgagct cctgctccag gccggcgcga cgattcttcc cgccaatccc
     4621 tccttctact cccgcccgcg gagcgtcgcg caggtcgcgg ataccgtggt cgcgcgggtg
     4681 ctggatcacc tgggcgtggc gcataagctg gtggcgcggt ggcaggagga acgggaatag
     4741 cgggccggga gggctgaatg ttgaaccctg gcctccagaa tccgccgtct tgatttccat
     4801 gttcgccacc ctccgcaaat gggcgtcctt cgttacgttc tcgcatacgg tgtttgcgct
     4861 gccgtttgcc cttgccgcca tggccgtggc cgcccgcgac catcgcggct ggcccggctg
     4921 gcgggtgttc ggcctgatcc tcgccgccat ggtctgcgcc cgcacttgcg cgatggcctt
     4981 caaccgcatc gtggatcgca aatttgacgc cctcaacccc cgcaccgcgc gccgccatct
     5041 gcccgcggga cacatcaccc tggccagcgc cgcgactctc tgcgccctgt cggccgccgg
     5101 gttggtgggg gcgagctatc tgcttaaccc gatctgcttc tacctcgcgc cggtcgcgtt
     5161 ggtggtcatc tgcttttatt cgctgaccaa gcggttcacg gattacaccc atgtctttct
     5221 ggggttgtcc ctggcgctgg ccccggtcgg cgcgtggctg gcggtcaagg gcctggagat
     5281 ctcgcgctgg gaaatcctcc agatgcttgt gctggccgcc gtcgtggttc tttggctcgt
     5341 cggcttcgac atcatctatg ccttgcagga ttacgagttc gaccgcgcgc aggggctgca
     5401 ctcgctcgtc gtcgcctggg ggcccgcgaa tgccttgcgg gccgccttcc ttgcgcacgt
     5461 gctgatgtgc ggcctgctgt ttgtctttgg gctgctctgc cggttccgca ttgcgtatat
     5521 catcggctgg gtgctcatcg ttggctgcct ggtgctcgaa cactggatcg cccgccggcg
     5581 cagccttaac tggatcaacc tcgccttttt ccgcctcaac gccgtggtca gcgccatctt
     5641 cctcgtggtc accctcgccg aggtcatatt tcagggcggc ttccggctgc gctgagcgcg
     5701 cccgggccca ggcgcgcccc gtcccaaccc gcgccgcctt ctgcggaatc ggccgggctg
     5761 atgagccggc gcggcggcgg gggcggcgcg gcgcccgcgg gccggggttt cggagggcgg
     5821 ggcggagctt atcggttgcg ggccaattgc cgggccagcc ggagggccgc caccatgctg
     5881 gaaggattgg ccttgccctg gccggcgatg tcgtaggcgg tgccgtggtc gggcgaggtg
     5941 cggatgaaag gcaggccgag cgtccaatta accccggttt cgaaggcgat catcttgagc
     6001 ggcgccaatc cttggtcgtg gtacatcgcc accaccgcat cgaactcgcc cttgaaggca
     6061 tggtaaaaca gcgcgtcggc ggccagcggg ccggccacgt ccaactgccg gctttgggcc
     6121 gcgcgcacgg cgggggtgat gatcgtctgc tcttccgtgc ccatttcgcc gccttcgccc
     6181 gcatggggat tcaggccgca gacggccacc cgcgcgcgtg gcaggccgag gtcgcggcag
     6241 gcttcggcag ccaactcgat gacctgctcg atcttggcct gcgtgaggtg gtcggatacg
     6301 cgtttgatcg gcagatgggt ggtggccagc gccacgcgca gccagcggcc gcggtcgtcg
     6361 tggccgagca gcatcatggc cgtgcgctgg gcgtgggcga gttgggaaag catttcggtt
     6421 tggccgacga aaggctgccc ggcgcggaca atcgcttcct tgcttacggg cgcggtcacc
     6481 agcgcgtcga gttgccgccg ctggcagcgt tccgccccct cccggaccca ggcgtaggcc
     6541 gcgcgcgccg cggcgggcgc tcccgccggc aagcccgccg gcagcgcatc gggcagcgga
     6601 tggcacaggc agaaccgccc gggcgcatcc ccgccggcgt cggcctggag cggaaggttc
     6661 aatccaagct gcgcgttaag gcggcgcgtg tgttccgcat cgccaatcag caggtagcgc
     6721 gtgtcatcgg ccggcgcttc gagcgcgagg gccttgaggg tgacttcggg gccaatgccc
     6781 gtgatgtcgc ccagggaaat accaattcgg gcggtcataa tgaaagctgc gcagcccgcg
     6841 ccggccgggg tgggcctccg cccggcccgc cgcgcggtgg gcgtctaaaa gacctggacg
     6901 aacgtcttct ttttgagccg gtcaagccac aggttggcca ggcgggtttg ttccttgacc
     6961 agcaagtcct gttcgatttg ctcccggact tcagccagcg ttttgaaccg ttccgggcgc
     7021 ttgtcctcga cctgcatgag gtagaactcg accggcggcg gcagattggc cgccgcggcg
     7081 gcggactcca accggcgttc ttcgacgagg tcttcctttt tcagcgccag gtccaccacg
     7141 taatgacgcg ccaggaccgg cgccttgttg gagtattggc aaacccagta atcatccccg
     7201 gcggaccggc tcagcacgct gctgttttgc ccgacctgga gcgcggcggc cgtatcggcc
     7261 aggccgcggc tcaggcgcga caattcccac caccccaaat ctcccccttc ggttttctgc
     7321 gaaccctcgg aatgaatgct ggccatttcc gcaaacgtgg cgccttcttt gagcttcgtg
     7381 aggatgtctt ccgccagcgc ggcggtcgag gccgcctccg tttgcccggg acacttcagc
     7441 acaatcattc gcagtttgac ttcctcctgg actttgaact cctcgcggcg ggcgaggtag
     7501 tgctcttcca ccttatgcgg cgagatgatg atggaagagg agatgttttc ctggcgcagc
     7561 caggagataa tgattcgatt gcgaatttgc tggcgatacc gctccaaggt gacgccctcg
     7621 gcttgcagcg ttcgcataaa cgccacccgg tccccgccat agcgcgtgtg gatttcccgc
     7681 tcaatgtcct tgtcaatgtc tttgccaatg atcttgttgg catgctccag ccgctccttc
     7741 tgggcgaact tgctgcggaa atcctgcagg atcaactgcc gctcgatcaa ctgctcgagg
     7801 ttttcgttga gggccgcggt caccttctgc tccagcaatt ccggctggcc gcgatactgc
     7861 cgcaccagca gttcgcgggc tggggtcatc cggtcctgca gttcggcaaa cgtcacgacc
     7921 gaatcatgca ccacggcttg aatggcgttg atcaggtggg tttcggcgct ggcgcggggc
     7981 gtgaaactga atcccgccgc ggccaccaac atgatgctga gcgttctcat cggaaagcgc
     8041 gcccaatcta ggcgccgcgg gctggcgggt caagcgccgt catccgggct gccgtcatcc
     8101 gggggccagg aaagattgtg gacatcgtga ccccagtctt tcccagtttt gaatcaatag
     8161 acgctttcat cccggctcgc tcaacccgaa aagtggcttt gggggtagaa agagcgtttc
     8221 cctctcatct actgaacctt acgcgccgac aacaaaagac cctggcccgg ccgcccaggc
     8281 tgtgtataaa gagctttatc tgctgcaccg aaagctccat gatgcgttcg gaactccaca
     8341 ggcgcccgga cgcctgtatg acgtgatgaa accgttaatt gaaatccgca accgggtgcg
     8401 gaaataaacc tggaaagcat caaagcatga aaacgaatca atggctgggc atcctggcgg
     8461 cggcgttaag cggcgtatta ttggcaggct gccagagcac gccctccccc acccccgcca
     8521 aaggacaaat ctcccatcgg ccattcggcc ggaccccgga cggcaccgcg gtggacctct
     8581 ttaccctgcg caacgcaaac ggcgtcgagg ccggcatctg taattacggc ggcctggtga
     8641 tcttcctgaa agtgccggac cgccacggcc ggctggggga cgtcacgctc ggctatgaca
     8701 agctggagga ttacctcaag gacagcccgt atttcggcgc gctgatcggg cgctacggaa
     8761 accggattgc gaaaggcaag ttcacgctca acggccagga atacaccctg gcggtcaaca
     8821 acggcccgaa cgcgctgcac ggcgggttga agggctttga taaagtggtg tggcagccca
     8881 ccatccgggc cacccgcgaa ggcccggagc tggagctgaa ctacgtgagc cgggacggcg
     8941 aggagggcta cccgggcacc ttgcgcgtca ccacgatcta ctccctgacc cccgacaacg
     9001 cgctcaagat tcaataccgg gccacgacgg acaaggacac cgtggtcaac ctcacgcacc
     9061 attcctactt caacctcgcc ggccagggcg acatcctgaa tcaccaagtc atgctgccgg
     9121 ccgacacgtt cacgcccgtg gaccgcacgc tgatcccgac cggcgagctg cggccggtgg
     9181 cgggcacgcc gtttgacttc cgcaccccca ccgccatcgg cgcgcggatc ggccaggacg
     9241 acgagcagct taagttcggc ggcggctacg accataactg ggtaatcaac aagcccctgg
     9301 ggcaattggg cctcatggcg cgggtctatg acccggccag cgggcgggtg atggaagtgc
     9361 tgtccaccga accgggcctg cagttctact cgggcaattt cctcgacggc aaaaacatcg
     9421 gcaaaggcgg ctgggcctac aagttccgca acggcttctg catggagccg cagcattatc
     9481 ccaactcgcc caaccagccc aacttcccct cggtggtgct caaacccggc caggtgtatc
     9541 acaacaccat cctctaccgg ttctccgtgc agaaataacc gcgccgccgc gccgccggaa
     9601 gcgcccggcc ggtgcgccgg gcgcgaggat tgacttttgc gcccgaccgg ttgtactggc
     9661 gggcgcatgg gtgccatgca gacatttcca gacccgctgg acttggccag cctgaaagtg
     9721 cgcccgctgg ccgagcggga aagccggagc cggctggagc acatcctggt ggaccccggc
     9781 gcgccgccgc gcgcatgcgc gcccgaagcg ctcgccgccg tccacgccgc cgcccagcgc
     9841 ctcgcggcgg cccgccagcg ccaggccagc gtcatcctca tgtatggcgc gcacctagtg
     9901 aagaacgggg cgctggccat cgtcaatcgc ctgctcacgg acggctggat cacccacctg
     9961 gccaccaacg gggcgggcac catccacgac tgggaactgt ccttcctcgg ccgcacggag
    10021 gaaagcgtgc ggcaaaacgt ggccaccggc tcgttcggcg cctgggacga aaccgggcgc
    10081 tgccagcacc tggccctgct ggccggcgcg ctgcgcggcg aaggctacgg ccgcagcctg
    10141 gggcgcttca tcagcgaaga cggcgtgacc ctgcccgacc ccgcggcgct ggcgcaatcg
    10201 ctgcgcgagc gcccggatga cccgctcgcg cccgcgcggg cggacttgct gcaggcgatg
    10261 cgggcgcacc aactgccggc cgggcgaatc ctcgtgccgc accgctggaa ggaaacctcg
    10321 attctgggac aggcgttccg gcagaacgtg ccgctgacgg tgcatcccgg cttgggctac
    10381 gacatcatcg cggtccatcc catgttcaac ggcgcggcca tcggccgggc ggcggcggtg
    10441 gatttccggc tgttcagccg cgccgtggag gggctggacg gcggcgtggt gctgtccatc
    10501 ggctcggcgg tcatggcgcc gcaggtgttc gagaaaagcc tcagttgcgt caacaacctg
    10561 cgccttcagg cgggccgccc ggccgtgcgg gacctggagt tctacgtggt ggacctccag
    10621 gacggcggcc aatgggactg gtcccggggc gagccgcccc gctccagccc cgcctactac
    10681 ctgcgcttct gcaagagctt cgcgcgcatg ggcggccgga tgaactacgt ccaatgcgac
    10741 aacgtggcct tcctgcataa cctcctccac cacctgccgg cgccgcgccg ctaacccggc
    10801 cccgtctccc cggcgccccc ggcgcgccgc ccccccgccg cggcgccagg cgcggccatc
    10861 cggggtttga catcccggcc gggtgcccta gtttggcgga atgttcgatt cattaagcag
    10921 caagctggag aaggtctttc gggatctgcg gggcctgggc aagatcagcg aggccaacgc
    10981 cagcgcggcc ctccgggagg tgcggcaggc cttgctggag gccgacgtca acttcaaggt
    11041 cgcgcgcgac ttcatcgagc gcgtccgggc ccagtcgctc gggcaggagg tcatccagag
    11101 cattcaaccc gggcagcagg tcatcaagat catccacgac gaactggtgc aactgctcgg
    11161 cgcgacgaat gcgggcctgc agttgagcgg caaccccagt tgcgtgctga tggtcggcct
    11221 gcacggctcg ggcaagacga cctccagcgg caagc
//
LOCUS       DAOPQG010000113        11196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6679_length_11196_cov_15.493941, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000113 DAOPQG010000000
VERSION     DAOPQG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6679_length_11196_cov_15.493941"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            762..1667
                     /locus_tag="PKI07_11485"
     CDS             762..1667
                     /locus_tag="PKI07_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC51706.1"
                     /translation="MNRRQFLQVTSLAAAAGAWLPQAALSAAKPAGVGWPIGCFNRPW
                     IGSPDKPWGYDTALDGIQAAGYRLTGLLTRPGKDEPLLGSDATPEYLAGLKRRIAARG
                     LAVNIGALRIKSHLPLADQIQDTRRQIDNGQTVGVEFLLTFGVSQPSQYEDYYKLMRD
                     AAAYAQERRLKVVMKPHGGASGAAEEIQRCLAKVQHANFKIWFDAGNIIHYTGKDPVE
                     QLKPIAAQVTGFCAKDCEGQNGSVMLQFGAGKVDFPAVFTVLKEAGFQGPLMVECCAL
                     GETPEIVTAHARKNREYLERALAAL"
     gene            1737..2915
                     /locus_tag="PKI07_11490"
     CDS             1737..2915
                     /locus_tag="PKI07_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOC51707.1"
                     /translation="MRTTMWFARLSIGLAWLLAGAAAPAADRLTNDWSVPFNSISDSA
                     PAIGPDGTIYVGTWKGLLRALTPEGARKWTFAAGNEIKSAPAVGADGTIYFGSRDRKL
                     YAVHPRGQKRWEFLTGGWVDSSPALGHDGTVCFGSWDGNFYALNTNGVKQWVFPTGGA
                     IVSSPAIGADGAIYFGSHDRKFYALAADGRKKWEFATGDAIISSPALNGDQGLYFTSV
                     DGWLYALTLEGRLRWRLRTGGITESSPVIGPDGTIYLGVFAHFWAVSPEGKKRWQRFG
                     DPLYPFESTPFVLEGGIVGCVSRSGLLCYLTPKGEVEGKYGLSGYGYSSPTVGPTGQI
                     YTPDNRVPYGGFSALRAGVALARTPWPKFRGNIRNTGNILDGNLYTPDAPHPQPAPAP
                     "
     gene            3125..3970
                     /locus_tag="PKI07_11495"
     CDS             3125..3970
                     /locus_tag="PKI07_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOC51708.1"
                     /translation="MNQRLKNIRHVALDMDGTIYSGGTLFDSTRPFLALMAELGIGHT
                     FLTNNSSKSSRDYLARLGQIGIQAGPDQLYTSTMATIEYLREQMPQVRRLFVLGTASM
                     RREMEAAGFVLTADSAAEEPEAVVVGFDTELTYARLCRTAYWIRQGKPCIATHPDRVC
                     PTDQPTVLVDCGAVCAALEQAAGRGPDVVLGKPDPCMLRGILHRHALAPEQLAMVGDR
                     LYTDMAMARRAGALGVLVLTGEATAAQAARHQPPPDLVLAGLAELGERLRAARQTLSE
                     RIQNE"
     gene            3963..5468
                     /locus_tag="PKI07_11500"
     CDS             3963..5468
                     /locus_tag="PKI07_11500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOC51709.1"
                     /translation="MNNANTRSQLDPAAQRQATLKTLEQDPFDVLVIGGGIVGTGVAR
                     DAAMRGLRVALVEQHDFSFGTSSRSSRLLHGGLRYLAQGRIGLVHEASVEKKVVHHIA
                     PHLADPLPFIFPTYRGNKDWVLWQLKIGVKIYDLLCGGRNLGKSTWHRPQDILRRVPG
                     LVAVNLTGGVRYYDGFTNDSRLALDTLRSAAAQGATVINYCRYRDAKRNGHWECQVED
                     VLTGRTFPVRACAVVNATGPWADGLPHSTVKLRATKGIHLVVEHGRAPVTETVVMTEG
                     KRILFMIPWGERTIIGTTDTDYQGSLDDVRADTEDVAYVLRVANLFFPNSQLTTRDVI
                     SAWAGLRPLVADPSGKPSDVSRAHEIHNPEPGWWDVAGGKLTTYRLMAEQTVDQIVRK
                     LKKLKGTAVAVAPCRTAREPLLPPSETAGVSALTPPPFERGPVEHYVRREWAVHLDDV
                     MVRRTSWHWYHRDAASKAAQVAEWMGELLGWPPETRQAELERYTRMWGASY"
     gene            5678..6934
                     /locus_tag="PKI07_11505"
     CDS             5678..6934
                     /locus_tag="PKI07_11505"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOC51710.1"
                     /translation="MAKLTVKDLNVRGKRVFVRVDYNVPLDHASGQVVITDATRIEAT
                     LPTLRLLRQQGGKLILAAHLGRPKGQREPSMSLRPVADELARRLGSPVGFVEDCIGPQ
                     VEQKVSAMQPGEVLLLENVRYYREEEANDPAFAEKLAKVADVYVNDAFGAAHRAHAST
                     EGVARLVARRGGQCAAGLLMELELKFLGEELEQPARPFVVILGGAKVSDKIQVIDRLL
                     EKADALLIGGAMAYTFRLAQGHSTGKSLVEADKTGIAQAALAKAAQRGVKFLLPSDDV
                     VAEPVKTDKVDKKGKPVIDYQNVRVNTSLDCPADAAGLDIGPATVRAYAAEVARAKTI
                     LWNGPMGLFEDPRFAEGTFAVARAVADVTQQAAAKSIIGGGDSVKALNKARLGDKVTF
                     MSTGGGASLEFLEGKVLPGVAALSDK"
     gene            6967..7731
                     /gene="tpiA"
                     /locus_tag="PKI07_11510"
     CDS             6967..7731
                     /gene="tpiA"
                     /locus_tag="PKI07_11510"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOC51711.1"
                     /translation="MNKERKLIVAGNWKMNKTVAEALDLVRGLKLELANVKEVDVVVC
                     PPFTALEAVGKAVLDSNIRLGAQNMSEHNVGAHTGEIAAVMLKEFSVRYVILGHSERR
                     QDQKEADALIARKAAAVHGAALKPIICVGETLAERQAGEMQKVLETQVRGSLAGLTGE
                     QMEESIIAYEPVWAIGTGQTATTAQAQEAHAFIRGLLVKMFNETVARRVRIQYGGSVK
                     PSNARELMSQPDVDGALVGGASLEARSFADIIKNSI"
     gene            7741..8166
                     /gene="secG"
                     /locus_tag="PKI07_11515"
     CDS             7741..8166
                     /gene="secG"
                     /locus_tag="PKI07_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOC51712.1"
                     /translation="MGFLIGLLTVVMVLDCIVLMFLVLIQLPKKDAGAGLAFGGAATD
                     ALFGAGSGNVLTKITKYAAVLFFILTISLSAMQRVHHARTTSAFEEGLKTSPAATVPA
                     APAPAAPSTPAPAAPATPAPAEAAQPPATAPATPAPTNP"
     gene            8319..9593
                     /locus_tag="PKI07_11520"
     CDS             8319..9593
                     /locus_tag="PKI07_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51713.1"
                     /translation="MRDKLNRRQFLQATATAALGAPLLLRAAPARKSPNDRITLGVIG
                     TGTQGRALLNNFLAMPDTQVVAVCDVDTTRRDHHRRLVDEFYRIKGNTEYRGCPGYKD
                     FRDVLARPDIDAVVIAVPDHWHAYIAIAACKAGKDVYCEKPLSLTIGEARAMVTAARK
                     YDRVFQTGSMQRSSSEFRKACELVRNGRIGRVKQVIVDVGPPSMPCNLPAEPLEPGLN
                     WDLWLGPAPERPYNSVLSPRGVHTHFPDWRNYREYSGGMMTDWGAHHFDIAQWGLGMD
                     ESGPVEIIPPADPQATRGARFRYANGVEMIHGDSGGVLFIGTEGKILVNRGKFEATPA
                     ALAEEPLGDRALRLYNSYSHTKDFLDCMRSRQRPICDVEIGCRSVTVCHLGNLAYWNH
                     RRLRWDPAREQFIGDREANRWIHRPQRGPWKT"
     gene            9704..10312
                     /locus_tag="PKI07_11525"
     CDS             9704..10312
                     /locus_tag="PKI07_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOC51714.1"
                     /translation="MRITGGTAARRILRVPKGLAVRPTPDRVKQAVFNSLGGRVAGAR
                     VLELFAGTGALSLECLSRGAVAAVCVENSPRHAAVLRANFQAAGFPPGVLQTRVQDVF
                     AALTQLAAARERFDLILADPPYGEKNIGRRSTSLAQRLLDEAALPELLRVEGWLVLGH
                     ARRDTLSLPEHWQELKLLKHGDSFMRFLCPARAAGAPAAAAP"
     gene            10323..11093
                     /locus_tag="PKI07_11530"
     CDS             10323..11093
                     /locus_tag="PKI07_11530"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOC51715.1"
                     /translation="MKSKDEIVADWLPRYTGTALADFGKYILLVNFSNYVRRFAQWHN
                     VPVRGEDRPMLNATAEGITIINFGMGSANAATVMDLLVSVQPEACLFLGKCGGLKRKN
                     KLGDLILPIAAIRGEGTSNDYFPPEVPALPAFSLQKAISTTIRDHQRDYWTGTVYTTN
                     RRVWEHDESFKKYLRRIRCMAIDMETATIFITGFHNEIPSGALLLVSDQPMAPEGIKT
                     WQSDQRVTANYTDMHLHVGIDSLRQLINQGLTVKHLRF"
BASE COUNT         1913 a         3507 c         3894 g         1882 t
ORIGIN      
        1 atgtttccgc gcccaggctg cccgtgcgcg gagaggttag tattttcata atgtaggtcc
       61 tttctattgt tgttctaatt gtctgtctat tacgggccgg agagcgactc cgcttcctgc
      121 cattccttgg gctggaagtt ccagccgccc ccaccccaag ggtctggagg aaggctggcg
      181 ggcgaagggt cttcgcgcaa gggcaatttt ttggggttgg cggcggtggg cgggtgcgga
      241 gcgccgcgtg ggggcaccgc ggcctacagg gggcgagatg gagcgggggg cctgtgggtt
      301 gccgggagtg aggtgttgca ggaaggcgtg tgtcctgccg aggcgtcctt ccttctcctg
      361 taggccgcgt gccctcacgc ggcgcatggt ggccatttgc ccggcggctg agaaaaactt
      421 ccacgccccc gcccgggttg cctgcggcca agccgccgca aataacgaga ttgacgtccg
      481 ggcaggcatt tgatcggctc gggctatgcc tggcgaactt ccatgcccgg cctcccgcgc
      541 ccgcgccgct cattattcgc gtggcactgt gggcctcttc tcccccggcc cttatcttcc
      601 gcaggcggtg gagagggagg attgatattt tggggcgggt tgcccagggt ggcgcacgag
      661 cctcgccggc cctgggctgt ccggaggaac ccttgcagga tttctggccg gggcggcaga
      721 ttcggcttgt gcgcggcggc tgaatatcgc ccaatgcggg gatgaatcgc cgtcagtttc
      781 tgcaagtgac cagtctggcc gccgcggcgg gagcgtggct tccgcaggcg gccttgtccg
      841 ccgcgaagcc ggcgggggtg ggctggccga ttggctgttt taaccggccg tggattggca
      901 gcccggacaa gccgtgggga tatgataccg cgctggatgg gatccaagcc gccggttacc
      961 ggctgaccgg gttgctgacg cggccgggga aggacgagcc gttgctgggg tcggatgcca
     1021 cgcccgagta tctggcgggg ctcaagcggc gcattgccgc gcgcgggctg gcggtcaata
     1081 tcggcgcgct gcgaatcaag agccatctgc cgctggccga ccagattcag gatacccggc
     1141 ggcagattga caacgggcag acggtggggg tggagttttt gctgactttc ggggtcagcc
     1201 agccgagtca atacgaggat tactacaagc tgatgcggga cgcggcggcg tatgcgcagg
     1261 aacggcggct gaaggtggtc atgaagccgc atgggggcgc gagcggggcc gcggaggaga
     1321 ttcaacgctg cctggcaaag gtgcagcatg ccaatttcaa gatctggttc gacgcgggga
     1381 acattattca ctacaccggc aaggacccgg tggagcagct caagcccatc gcggcgcagg
     1441 tgacgggctt ctgcgcgaag gattgcgagg ggcagaacgg gtcggtcatg ctgcagtttg
     1501 gcgccggcaa agtggatttc ccggcggtgt tcaccgtgct gaaagaggcc ggtttccagg
     1561 ggccgctgat ggtggaatgc tgcgccctgg gcgagacgcc cgaaatcgtg acggcccatg
     1621 cgcggaagaa ccgggaatac ctggaacgcg cgctggccgc gctgtagggc ggcggcgggg
     1681 cgcgcgcctt tttgctcgtg cgcggcgggc gcggccgcta cgctgagggg gcatacatga
     1741 ggacgacgat gtggtttgcc cgcttgagca tcggcctggc gtggctgctg gcaggggccg
     1801 cggcgccggc ggccgaccgg cttaccaatg actggtcggt gccgttcaac agcatcagcg
     1861 attccgcgcc ggccatcggc cccgacggga ccatttatgt cggcacgtgg aaaggcctgc
     1921 tccgggcgct caccccggag ggcgcgcgca agtggacgtt tgcggcgggg aatgaaatca
     1981 agtccgcccc cgccgtgggc gcggacggga cgatttattt cgggagccgg gatcgcaagc
     2041 tttacgccgt gcatccccgg ggccagaagc gctgggagtt tctgacgggc ggctgggtgg
     2101 attcatcgcc cgccctgggg cacgatggca cggtctgctt cggttcgtgg gacgggaatt
     2161 tttacgcgct gaatacgaat ggcgtcaaac aatgggtgtt tccgacgggc ggcgcgattg
     2221 tctcatcgcc ggcgattggc gccgatggcg ccatctattt cggctcgcat gaccggaaat
     2281 tctacgccct ggccgcggac gggcgcaaga aatgggaatt tgccaccggc gatgccatta
     2341 tttcctcccc ggccttgaac ggggatcagg gcctttactt cacttcggtg gacggctggc
     2401 tgtatgcgct cacgctggag ggccgcctgc gctggcggct gcgcacgggc ggcatcaccg
     2461 agtcgtcgcc ggtgattggg ccggacggca cgatttacct gggggtcttt gcgcatttct
     2521 gggccgtcag ccccgagggc aagaaacggt ggcagcggtt cggagacccg ctttacccgt
     2581 ttgaatccac gccgtttgtt ttggaggggg gcatcgtcgg ctgcgtctcg cgctccggct
     2641 tgctgtgcta cctgactccg aaaggcgagg tggaagggaa atacgggctt tccggctatg
     2701 ggtatagctc gccgaccgtc gggccgacgg ggcagattta cacccccgat aaccgggttc
     2761 cttatggcgg tttctccgcg ctgcgcgccg gcgtggcgct ggcccgcacc ccctggccca
     2821 agttccgcgg caatatccgc aataccggca atatcctgga cggaaacctt tataccccgg
     2881 acgcgcccca ccctcaaccc gcgccggcgc cctgaccgcc atgcgcctcg cctccctccc
     2941 atgattgccg ctcaccccgg cccgggcaga ctcgcgggaa acccgcaacc gggccgggcg
     3001 gatcggcact gcaaaccggc ggagaggatg ggcaagattg gggattgaca gccggtgggg
     3061 gagcgggtac ttagtgcgtt aacgctcaac gatttaagtt ttgaatacga ccacgaagca
     3121 ggccatgaat cagcggctga agaatatccg ccatgtggcg ctggatatgg atgggaccat
     3181 ttacagcggc gggacgcttt ttgattccac ccggccattt ctggcgttga tggcggagct
     3241 gggcatcggg cacacctttc tgaccaataa ttcctccaag agttcccggg actacctggc
     3301 gcgcctgggc cagatcggga ttcaggccgg tcccgaccag ctttatacgt ccacgatggc
     3361 gacgatcgaa tatctgcgcg agcagatgcc gcaggtgcgg cgcctgtttg tcctgggcac
     3421 ggccagcatg cggcgggaga tggaggcggc cggctttgtc ctgaccgcgg acagcgccgc
     3481 cgaggagccg gaggcggtgg tggtgggctt cgatacggag ctgacctatg cgcggctctg
     3541 ccgcacggcc tactggatcc gccagggcaa gccctgcatc gccacgcatc cggaccgggt
     3601 ttgcccgacg gatcagccca ccgtgctggt ggattgcggc gcggtttgcg ccgcgctgga
     3661 gcaggcggcg gggcgcgggc cggacgtggt tttgggcaaa cccgatccgt gcatgttgcg
     3721 gggcatcctg catcggcacg cgctggcgcc ggagcaactg gcgatggtgg gcgaccggct
     3781 ctacacggat atggcgatgg cgcgccgggc gggcgcgctg ggcgtgctgg tgttaaccgg
     3841 cgaggccacc gccgcgcagg ccgcccggca tcaacccccg cccgacctgg tgctggccgg
     3901 cctggcggaa ctgggcgagc gcctgcgcgc cgcccgccaa accctcagcg aaaggattca
     3961 aaatgaataa cgcaaatacc cgcagtcagc tcgacccggc cgcgcagcgg caggccaccc
     4021 tcaagacttt ggagcaggat ccgtttgatg tcctggtcat cggcggcggc atcgtcggca
     4081 ccggggtggc ccgggacgcg gcgatgcgcg gcctgcgggt cgcgctggtg gagcagcatg
     4141 acttttcctt cggcaccagc agccgctcca gccggttgct gcacgggggg ctgcgctacc
     4201 tggcgcaggg gcgcatcggg ctggtgcacg aggcgagcgt cgaaaagaaa gtggtgcacc
     4261 atattgcgcc gcacctggcg gacccgctgc cgttcatctt tcccacctac cggggcaaca
     4321 aggactgggt gctgtggcag ctcaagatcg gcgtgaagat ttatgatttg ctgtgcggcg
     4381 ggcgcaacct gggcaaatcc acctggcacc gcccgcagga catcctgcgc cgggtgccgg
     4441 ggctggtggc tgtcaacctc accggcgggg tgcgttatta tgacgggttc accaatgact
     4501 cgcggctggc gctggacacc ctgcgctcgg cggcggccca gggcgccacg gtgatcaatt
     4561 actgccgcta ccgggacgcg aagcgcaacg ggcattggga gtgccaggtc gaagacgttc
     4621 tgaccgggcg gaccttcccg gtgcgcgcct gcgcggtggt caatgccacc ggcccctggg
     4681 cggacgggtt gccgcacagc accgtcaagc tccgcgccac caagggcatc cacctggtgg
     4741 tcgagcacgg gcgggcgccg gtgaccgaga cggtggtcat gaccgagggc aagcggattc
     4801 tctttatgat tccgtggggc gagcgaacca tcatcggcac cacggacacc gattaccagg
     4861 gatcgctgga cgacgtgcgg gccgataccg aggacgtcgc ctatgtgctg cgggtggcca
     4921 acctgttttt cccgaacagc cagttgacga cgcgggatgt catcagcgcc tgggcggggc
     4981 tgcggccgct ggtggccgac ccgagcggca agccgtccga cgtgtcgcgc gcgcatgaga
     5041 ttcataatcc ggagccgggc tggtgggatg tggcgggggg caagctgacc acctaccggt
     5101 tgatggccga gcaaacggtg gatcagattg tccggaagct gaaaaagctg aaggggacgg
     5161 cggtggcggt cgcgccgtgc cgcaccgcgc gggagccgtt gctgccgccc agcgaaacgg
     5221 cgggcgtcag cgcgttgacg ccgccgccct tcgagcgcgg cccggtcgag cattacgtgc
     5281 ggcgcgaatg ggcggtgcac ctggacgacg tcatggtgcg gcggaccagt tggcattggt
     5341 accaccgcga cgccgccagc aaggcggcgc aggtcgccga gtggatgggc gaactgctgg
     5401 gctggccgcc ggaaacccgc caggccgagc tggagcgcta tacgcggatg tggggcgcca
     5461 gttattgacc ccccggccgc ctccgggcgg cgcgcggaag cctcccgccc gcgaggcggc
     5521 agctcgcggg ttcagggagt gggcggctcc tcccgggccg cgagtccagg cccgcggcgg
     5581 ccgttaaaaa gggcgttaaa aatcggtgtg gaatgagttt gactcggacc ggggcgggcg
     5641 tatgctggac ggtcctggtt gggaagatca gaactttatg gcaaaactga cagttaaaga
     5701 tttgaacgtg cgcggcaagc gcgtgtttgt gcgcgtggat tacaacgtgc cgctggacca
     5761 cgccagcggc caggtggtca ttacggatgc gacgcgcatc gaggccaccc tgccgacgct
     5821 ccggctgctc cggcagcagg ggggcaagct cattttggcc gcccacctgg gccgccccaa
     5881 aggccagcgc gagccgtcca tgtcgctgcg ccccgtggcg gacgagctgg cccggcggct
     5941 gggctcgccc gtcgggtttg tggaggattg catcggcccg caggtcgagc agaaagtcag
     6001 cgccatgcag cccggcgagg tgctgctgct ggagaacgtg cgttattacc gcgaggaaga
     6061 ggccaatgac ccggcctttg cggagaagct ggccaaggtg gccgatgtct atgtcaacga
     6121 cgcgttcggc gcggcgcacc gcgcgcacgc ctcgaccgag ggtgtggccc gcctggtggc
     6181 tcggcgcggc ggccagtgcg cggccggcct gctgatggaa ttggagttga agttcctggg
     6241 cgaagaactc gaacagcccg cccgcccctt cgtcgtcatc ctgggcgggg ccaaggtttc
     6301 ggacaagatt caggtcattg accggctgct ggaaaaggcc gacgcgctgc tgattggcgg
     6361 agcgatggcc tacacgttcc gcctggcgca ggggcactca acgggcaagt ccctggtgga
     6421 ggcggacaag accgggatcg cccaggcggc gctggccaag gccgcgcagc ggggcgtcaa
     6481 gttcctgctg ccgtctgatg acgtggtggc ggaaccggtg aaaaccgaca aggtggacaa
     6541 gaaaggcaag cccgtgattg actaccagaa cgtgcgcgtc aacacgagcc tggactgccc
     6601 ggcggacgcc gccggcctgg acattggccc ggccacggtg cgggcctacg ccgccgaagt
     6661 cgcccgggcc aaaaccatcc tctggaacgg cccgatggga ttgttcgagg acccgcgttt
     6721 tgccgagggc accttcgcgg tggcccgcgc cgtggcggac gtgacgcagc aggccgcggc
     6781 caagagcatc atcggcggcg gcgacagcgt gaaggcgctg aacaaagccc ggctggggga
     6841 caaagtcacc ttcatgagca ccggcggcgg tgccagcctc gagttcctcg aaggcaaggt
     6901 cctgcccggg gtggccgcgt tgagcgacaa gtaaggatgc ccgcggcgaa gaacgattta
     6961 cgacccatga ataaagaacg aaagctgatt gttgccggca actggaagat gaacaagacc
     7021 gtggccgagg cgctcgacct ggtccgcggt ctgaaactcg agctggccaa cgtcaaggag
     7081 gtggacgtcg tcgtctgccc gcccttcacc gcgctggaag ctgtgggcaa ggcggtgctg
     7141 gattccaata tccggctcgg cgcgcagaac atgagcgagc acaacgtcgg ggcgcacacc
     7201 ggcgagattg ccgccgtcat gctcaaggag ttttcggtcc ggtatgtcat tctgggtcat
     7261 tccgagcggc ggcaagatca gaaggaagcg gacgcgctga ttgcccggaa agccgccgcc
     7321 gtgcatggcg cggcgctcaa gccgattatt tgcgtcgggg aaacgctggc cgagcggcag
     7381 gccggggaaa tgcagaaagt gctcgaaacg caagtgcgcg gcagcctggc ggggctgacc
     7441 ggcgaacaga tggaggaaag catcatcgcg tacgagccgg tctgggcgat cggcaccggg
     7501 cagacggcca ccacggcgca ggcgcaggag gcccatgcgt ttatccgcgg cctgctggtg
     7561 aagatgttca acgaaaccgt tgcccggcgc gtgcgcatcc agtatggtgg cagcgtcaaa
     7621 ccgtccaacg cgcgcgagtt gatgagccag cccgatgtgg atggcgcgct ggtgggcggg
     7681 gcctcgctgg aggcgcgcag ctttgcggat atcatcaaaa attcaatcta gtaaacgtat
     7741 atgggtttcc ttattggcct gttaaccgtg gtgatggtgc tggactgcat cgtcctgatg
     7801 ttcctggtgc tgattcagtt gccgaagaaa gatgcgggcg ccgggctggc gttcggcggc
     7861 gcggcgaccg atgccttgtt tggcgccggt tccggcaacg tcctgaccaa gattaccaaa
     7921 tacgcggccg tgctcttctt tatcctgacg atctcgcttt cggcgatgca gcgcgttcac
     7981 cacgcccgca ccaccagcgc attcgaagag ggcctcaaaa ccagcccggc ggcgaccgtt
     8041 ccggcggctc ccgcgccggc ggctccgtcc actccagcgc ccgcggcccc ggctactccg
     8101 gcgcccgcgg aagccgcgca accgcccgca accgctccgg caacgccggc gcccaccaac
     8161 ccctagcggc agtcgtccgc cgggttgcgg ccagcgccgc cgcgctcctg cctcgcccgc
     8221 cgtccggaag cgggggatcg tccgcctttt cctgacggcg cccgtccgga gaaattcagt
     8281 tgccggagcc accaaaacgg caaacaatct ccgctgttat gcgagataag ttgaatcggc
     8341 gccaattcct ccaggccacc gcaactgccg cgctgggcgc gccgctgctg ctgcgggccg
     8401 cccccgcccg gaaaagcccc aatgaccgca tcacgctggg cgtcattggc accggcaccc
     8461 aggggcgcgc cctcctgaac aacttcctgg ccatgcccga tacccaggtc gtggcggtgt
     8521 gcgatgtgga caccacccgg cgggatcatc accgccgcct ggtggatgag ttctaccgca
     8581 tcaagggcaa taccgaatac cggggctgcc ccggctacaa ggatttccgc gacgtgctcg
     8641 cgcggccgga tattgacgcg gtggtgattg ccgttcccga ccattggcac gcctatatcg
     8701 ccatcgccgc ctgcaaagcc ggcaaggatg tctattgcga aaagccgctt tccctgacca
     8761 ttggcgaggc ccgcgccatg gtcacggcgg cccggaaata tgaccgggtc tttcaaaccg
     8821 gctcgatgca gcgatcatcc agcgagtttc gcaaagcctg cgaactggtt cgcaacgggc
     8881 gtattggccg ggtcaaacag gtaatcgtgg acgtcggccc gccttccatg ccgtgcaacc
     8941 tgccggcgga acctctggag cccggcctca actgggatct ctggctgggg cccgccccgg
     9001 agcgccctta caattctgtg ctcagccccc gcggggttca tacgcacttc cccgactggc
     9061 ggaactaccg ggaatactcg ggcggcatga tgacggattg gggcgcgcac cacttcgaca
     9121 tcgcccagtg gggcctgggc atggacgaga gcggcccggt ggaaatcatc ccgcccgccg
     9181 acccccaggc cacccgcggc gcgcgcttcc gctacgccaa cggcgtcgaa atgattcatg
     9241 gcgacagcgg cggggtgctc tttatcggca ccgagggcaa aatcctcgtc aaccggggca
     9301 agttcgaggc cacgcccgcg gccctggccg aagaaccgct gggcgatcgc gccctgcgcc
     9361 tctacaacag ctacagccat accaaggact tcctggactg catgcgcagc cgccaacgcc
     9421 cgatctgcga cgtggagatc ggatgccgct ccgtcactgt ctgccacctg ggcaacctgg
     9481 cttactggaa ccatcggcgc ctgcgctggg acccggcccg ggagcaattc atcggcgacc
     9541 gcgaggccaa ccgctggatc caccgccccc agcgcggccc gtggaagacg taaacccgcg
     9601 gtaaaaacca atccccgccg gcatcccgct aacagcgcaa accggacggc cagggcctcc
     9661 atttcccgcg tgcctttccg cggagcgcgg gttaaagttg ggcatgcgca tcacgggtgg
     9721 aaccgcggcc cgccgaattc taagagtgcc gaaaggactg gccgtgcgcc ccacgcccga
     9781 ccgggtcaag caggcggtct tcaacagcct gggcggccgg gtggcgggcg cgcgggtgct
     9841 ggaactgttt gccggcaccg gggcgctgag tctggaatgc ctgagccgcg gcgccgtggc
     9901 ggcggtatgc gtggagaact caccccgtca tgcggcggtg ttgcgcgcga acttccaggc
     9961 agccggcttt cctcccggag ttttgcaaac gcgggtgcag gatgtgttcg cggcgctgac
    10021 gcaactggcc gcggcgcgcg agcgcttcga cctgatcctg gcggacccgc cgtatggcga
    10081 gaagaatatc ggccggcgct cgacctcact ggcccagcgg ttgctggacg aggcggcgct
    10141 gccggagctg ctgcgggttg aaggctggct ggtgctgggc cacgcgcggc gcgacaccct
    10201 gagcctgccg gagcattggc aggagctgaa gctgctcaag cacggcgaca gtttcatgcg
    10261 cttcctgtgc ccggcccgcg cggccggggc gccggcggcg gcggcgcctt gaggcaagaa
    10321 ccatgaaaag caaagatgag atcgtggcgg actggctgcc gcgctacacg ggcacggcgc
    10381 tggcggattt cgggaaatac attctgctgg tgaacttcag caactacgtg cggcggttcg
    10441 cccagtggca taatgtgccg gtgcgggggg aggaccgccc catgctcaac gcgacggcgg
    10501 aggggattac catcatcaat tttggcatgg gcagcgccaa cgcggcgacc gtcatggacc
    10561 tgctcgtctc cgtccagccc gaagcctgtt tgtttctggg caaatgcggc gggctcaagc
    10621 ggaagaataa actgggggac ctgatcctgc cgattgcggc gatccgcggc gagggaacct
    10681 cgaacgacta tttcccgccg gaagtgccgg cgctgcccgc cttcagcctg caaaaggcca
    10741 tctccaccac catccgcgat caccagcgcg attactggac cggcacggtc tataccacca
    10801 accgccgcgt atgggagcac gacgagagct tcaaaaaata cctgcggcgc atccggtgca
    10861 tggcgattga tatggagacg gcgacgatct ttatcacggg tttccacaac gagatcccca
    10921 gcggcgcgct gctgctggtg tccgaccagc cgatggctcc ggaaggaatc aagacctggc
    10981 aaagcgatca gcgcgtgacc gccaactaca cggacatgca cctgcacgtg ggcattgatt
    11041 ccctgcgcca gctcatcaac cagggcctga cggtcaagca cttgcggttc taggcgaaga
    11101 gcgccgggct atctgccccc cggccggggc cattcagcgc ctcataccac ttctggcacg
    11161 taaatgcact attctccgct gcggtagcgt ttactt
//
LOCUS       DAOPQG010000114        11146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6712_length_11146_cov_14.739879, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000114 DAOPQG010000000
VERSION     DAOPQG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11146
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6712_length_11146_cov_14.739879"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKI07_11535"
     CDS             <1..213
                     /locus_tag="PKI07_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51716.1"
                     /translation="AVAPASTAAHKTVPSPSFQAMYKGSTSEARQEHMENKHESPLSL
                     LCTPVVHSLMGALGWLGRVGRVWARA"
     gene            642..1085
                     /gene="rpiB"
                     /locus_tag="PKI07_11540"
     CDS             642..1085
                     /gene="rpiB"
                     /locus_tag="PKI07_11540"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOC51717.1"
                     /translation="MKIAIASDHAGFQYKEKIREFLAGLGHHVEDFGTDSEAAVDYPL
                     FIRPAAEAVARGEFDRGIVLGGSGNGEAIVANRVKGIRCALCWNVDSARLGRQHNNAN
                     LLSLGQRMMSLDTALQIVRVWLETPFEGGRHARRIELIDAPPPPR"
     gene            complement(1209..1784)
                     /locus_tag="PKI07_11545"
     CDS             complement(1209..1784)
                     /locus_tag="PKI07_11545"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOC51718.1"
                     /translation="MTTLTTPISESVVRALKVGEEVLITGTVFTGRDAVHKYLYDGGA
                     LPAGVSFQNGILYHCGPVIIKDEAGNWKCVAAGPTTSIREEPYQWRVIRDFGLRGVIG
                     KGGMGEKTVAACREHGCVYLHAVGGAAQVLAACVQRVPNVYFMEEFGAPEAIWEFQVA
                     SFPAVVTIDAHGHSLHQEVLAASQAALAKYL"
     gene            complement(1781..2311)
                     /locus_tag="PKI07_11550"
     CDS             complement(1781..2311)
                     /locus_tag="PKI07_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51719.1"
                     /translation="MEPLPSACESTPARTPQTIVWFRVYAAVMTGLYALCVLAAPLVF
                     LAGTRRMGEEGLLLKIQAGVLLLMGLVFALAFALALVLPSKPWSWVYGLAQTFHTCKA
                     GARASCPLSPFGGCKRARCPRSGLRVFRAGREISRLVRSCLGMTSCCLLPVVIPLLIY
                     WLKPGVKNWFNPGASL"
     gene            complement(2315..3193)
                     /locus_tag="PKI07_11555"
     CDS             complement(2315..3193)
                     /locus_tag="PKI07_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOC51720.1"
                     /translation="MNALEDSLLELIRRTSAEIPDDVHQALLASLAREQQGTIAASAM
                     QIIQRNVALAKNKSQPICQDTGSILFYVDCPRGLDQLDFARAARAAVKRATQKGYLRQ
                     NSVDPITGKNDGTNVGPGSPALHFDQHAAPETRVRLILKGGGCENVGAQYALPHEKLK
                     ASRDLDGCRRVILDAVLQAQGKGCGPGVLGVCIGGDRATGYEFSKRQLLRKLGEANPN
                     PALDKLERDVVETANKLGIGPMGFGGRTTLLGAWIGVLNRLPASYFVSISYMCWAFRR
                     QGVTLDPNGGIAGWLY"
     gene            3782..5002
                     /locus_tag="PKI07_11560"
     CDS             3782..5002
                     /locus_tag="PKI07_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOC51721.1"
                     /translation="MNLFAPRILLPGLASLALICPAIPAKAQTNAPPAAFPPTVPGVV
                     IAHRPAASGIYIGSPSLAVLSNGHYIASHDEFGPKSSEHRRAISHIFLSRDRGATWGK
                     ISTLDGAFWSSLFVHRNALYLLGPDKHHGNLLIRRSLDGGVTWTSPTNPATGLLRDDG
                     QYHCAPMPVIEHAGRLWRAMERRIPPIAWGINYRAGMFSIPVDADLLQATNWTHSNFL
                     PSDRAWNGGDMGAWLEGNAVVSPAGELVNVLRVQTRSPEEKAALVHLSPDGRTATFDP
                     ATDFIRFPGGAKKFTLRFDPETKRYWALASIVRERHRAPDPGRIRNTLALTTSTNLLN
                     WEVRCILLYHPDTAKHGFQYVDWLFDGQDIIAVCRTAFDDGQGGAHNNHDANYLTFHR
                     FANFRRLTMANSVP"
     gene            complement(5103..5873)
                     /locus_tag="PKI07_11565"
     CDS             complement(5103..5873)
                     /locus_tag="PKI07_11565"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOC51722.1"
                     /translation="MEVDISKAIVRGYAAKLESCLDCDLVVVGAGPSGLLCAAHVARA
                     GRNVAVFDRKLAPGGGVWGGAMLFNEVVIQQAAVAILEELGVRHTRVDDRFVRADSVE
                     LAASLIYRAVHAGAKVFNGVSVEDVIFKGDRIGGVVINYTPILLNNLHVDPLSMRAKV
                     VLDGTGHGAELTARAAQKAGIRLETPTGGLMGEKPMWAEMGEKMTVENTRRVYPGLYV
                     SGMAANGVFGSARMGPIFGGMLLSGQRAAKLILEELGR"
     gene            complement(5979..7154)
                     /locus_tag="PKI07_11570"
     CDS             complement(5979..7154)
                     /locus_tag="PKI07_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOC51723.1"
                     /translation="MRNNRLPWMVLIFCALALGRPAPAAAQTLYTEDGEPTGLEEEIR
                     WRVNRGRFDTVSENQLRGSSYTDVPATAGPLAPNQKLALAARHHSEDMAKQNVFQHET
                     VVGSAYYNPVTQSNFWDRITAEGYVWNGIAENIAAGYVGAEAVYMGWWHSEGHRLNMY
                     HVNLREIGNGYYYWEASDYRRYYTMDLGVSGVDYFFTDTLFRDANSNGVYEAAEAVGG
                     VAVRLLVDGSPTSHYDISSSVGSFAIPIGAIVPSSTVQVVLSNTTAATLALTIPVDYW
                     TNRALTLAPGESRVCGTFTTGATWGGRRNIGLRNITPPAPPAAPYLHLARLGADMQLE
                     WLSAVGVQYQPLWSTNFQVWNNLTANPLNGTGTNLTCLDVGATAHERRFYRLQVIQP"
     gene            complement(7278..9635)
                     /locus_tag="PKI07_11575"
     CDS             complement(7278..9635)
                     /locus_tag="PKI07_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51724.1"
                     /translation="MSQNRNQERAWWVAALLVLAVLAVYLPVVELDFINLDDDDYVTA
                     NPQVRQGLTGGAVRWAFTRCHAANWHPLTWLSHMLDCQIYGLRPAGHHATNALFHAAN
                     ALLLFLWLRSLTGAFWRSLLVAALFAVHPLRVESVAWVAERKDVLSAFFGLLSLWAYT
                     AYASRRRAREPERAGAAPAGGGAGGVKGAALVCYLLSVGCLALGLMSKPMLVTWPLVM
                     LLLDYWPLGRIGKWNAPRGGAAVFGRRRWVGLLVEKAPFFALSAASCGVTVWAQKAGG
                     ALVELQSLPLGARLENAVGGYGRYLGKLVWPADLIVHYDSPAFASAAALAAALGALAA
                     ATLLAWGQRKKRPYLLMGWLWYLGMLVPVIGLVQVGSQTIADRYTYLPTVGLLIIAAW
                     GAGELAGAGRRRRWVVAGAAAAALIACARATGAQLLYWQNSETLFRHTLACARSNWLA
                     WNNLGAFLAGQRALRPAELCYRVALEIRPQSEVSWRNLGGVLRDQNRNEEAIAVYEAA
                     LRQDSRFVTVRNGLATALAASGRLAEARAQCREAARLDPAAAEPYSNLGALLARERQW
                     DEAAAAFRQALARDPVMSEARCGLGGVLANQGHYEEAVRELAGLLRLQPTNTAARLQL
                     AGVWLVRDRLDEAQREYAEVLRHDPRNATAHYRLAQLLLQRHDPRGAIEHYRAALAAR
                     PGYGEALNNLAWTLATHPDPQFRDGPEAVRLAERAVRLTDQRQALMWGTLAAACAEAG
                     RFPEAVAAAERARELAEKANDADAAAFNARMRELFRAGQPYREAP"
     gene            9727..10938
                     /locus_tag="PKI07_11580"
     CDS             9727..10938
                     /locus_tag="PKI07_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOC51725.1"
                     /translation="MKTTAARHLSFSRSSALNSTGFGARSGALTLTAALLLLWLAAGP
                     GYAAAPAAAPEARGIWVHLTHFNPDPDRGKAEVKAFADRYADAHFNFVLPWVTSDYIA
                     ALTDPAYQTNQPTARWDALGELVRCARERHLQVHLWYSFTDYRFARSPDFNPRHGGDP
                     AWAARRVTPPAQAGQSPQVTSPMNDVCPMHPEARAWQLKQVDSLLDRYPGISIHIEEP
                     GYGTTGNCACDLCRSLYRKIHGTDIVDAINSPAAEDFRCLGTTEFIRALRAKLEKRNP
                     RPIFSVNGGPYWSSDRKLGRDWKRWAELGWLDYYASQNYTPNLEFFTRLTQTILKDLQ
                     PCPVFIGIGVKWSGGETPMPTVLQEIELARKLGAAGVILFSASAITDSDLAALKAGPF
                     QAPADYPSPQR"
BASE COUNT         1958 a         4013 c         3355 g         1820 t
ORIGIN      
        1 gccgtcgccc cggcatccac cgccgcccac aagaccgttc cttctccgtc ctttcaagcc
       61 atgtacaagg ggagtacatc ggaagcccgg caggagcaca tggaaaataa gcatgagtcc
      121 cctttgagcc tcctttgtac ccccgttgta cacagcctaa tgggagcgtt aggctggctg
      181 gggcgggtgg gacgggtttg ggcgcgagcc tgagccggat tggagggatg gacgacctgc
      241 ggatgatgta aaacgagatt ccaaggcggg agggagagga cgcgaacgtt cggcaaagcc
      301 gggacgagcg gccgggtgtt gtcgcgtgcc ttggtttttc gacgggatgg ggccaaggct
      361 ggcggggaca gttctcctct ttggcggagg ctgccgaatg gtgactgctt acgttgccct
      421 catcgcgggc ggcgtattcg cccgattggg gtgaggttgg gcggcggttg agcgcttcgc
      481 ccggagcggg cgttttgcgg cgtggcaaag gcgttgggtt ggaaaaaccc ttttccccct
      541 caactcccgg aactcaggcg ccgacaacaa acggccctgc caaatggagg ctggcgcgtt
      601 ttctccgggt gtggcaggct gggcggcgga ttatgcagca cctgaaaatc gccattgctt
      661 cggaccatgc cggcttccaa tacaaagaga aaatccggga gttcctcgcg gggctgggtc
      721 accacgtcga ggacttcggg acggattcgg aggcggcggt ggattatccg ctgtttatcc
      781 gccccgccgc ggaggccgtg gcgcggggcg aatttgatcg cggcattgtg ttgggcggct
      841 cgggcaatgg cgaggccata gtggccaacc gggtcaaggg gattcgatgc gcgttgtgct
      901 ggaatgtgga ttcggcgcgg ctgggccggc agcataacaa tgccaacctg ctctcgctcg
      961 gccagcgcat gatgagcctc gacacggcgc ttcagattgt ccgggtctgg ctggaaaccc
     1021 ccttcgaagg cggccggcac gcccgccgta tcgagctgat tgacgccccg ccgccgccgc
     1081 ggtaaggccg gttctcaatc cgccgccggg cggggccgcc tccgcgcgcg cccggacggg
     1141 ttccgccgcg agggcgccac gcaagcaagc cgcgccggtc cgtccattcc ccgggcgccc
     1201 gccgggcgct acaggtattt cgccagcgcg gcctggctgg cggcgaggac ttcctggtgc
     1261 agggaatggc cgtgggcgtc aatggtcacg accgcgggga agctggcgac ctggaactcc
     1321 cagatggctt cgggggcgcc aaactcctcc atgaaataca cgttgggcac gcgctggacg
     1381 caagcggcga gcacctgcgc cgccccgccc accgcgtgca gatagacgca gccgtgctcc
     1441 cggcaggccg cgacggtttt ttctcccatg ccgcccttgc caatcacccc gcgcaggccg
     1501 aaatcgcgga tgacccgcca ttggtagggt tcctcccgaa tcgaagttgt cggcccggcg
     1561 gcgacgcatt tccagttgcc cgcctcgtcc ttgatgatga ccgggccgca atggtaaaga
     1621 atgccgttct ggaaacttac gccggcgggc agcgcgccgc cgtcatacag gtatttgtga
     1681 acggcgtcgc ggcctgtgaa caccgtgccg gtgatgagca cttcctcgcc cactttgagc
     1741 gcgcggacga cggattcgga gattggagtt gtgagagtag tcataagctc gcgcccgggt
     1801 tgaaccagtt tttcaccccg ggtttcaacc aataaatgag caagggaatg accaccggca
     1861 acaggcagca actggtcatg ccgaggcagc tacgcactag ccgggatatt tcacgccccg
     1921 cacgaaatac ccgcaggccg gagcgcgggc atcttgcccg cttacagcct ccaaacgggc
     1981 taagcgggca agatgcccgc gctccagcct tgcaggtgtg aaatgtctgg gctaggccgt
     2041 agacccagct ccacggcttg ctcggaagca ccagcgccag cgcaaaggcg agggcaaaaa
     2101 caagccccat cagcaaaagc acgcccgctt gaattttcag cagcagtccc tcctcgccca
     2161 tgcggcgagt cccggcgaga aacaccagcg gcgcggccag cacgcacagc gcgtaaaggc
     2221 cggtcatgac cgcggcatag acccggaacc aaacgatggt ttgcggggtg cgggcggggg
     2281 ttgattcgca agcggaaggc aggggttcca taatttaata aagccagccg gcaatgccgc
     2341 cgttcgggtc gagggtgacg ccctggcggc ggaaggccca gcacatatag ctgatgctga
     2401 cgaaatacga cgcgggcagg cggttgagca cgccgatcca ggcgcccagc agcgtggttc
     2461 taccgccgaa acccatcggg ccgatgccca gtttgttggc cgtttccacc acgtcgcgct
     2521 ccagcttgtc gagcgcgggg tttggattgg cctcgccgag cttgcgcagc agttggcgtt
     2581 tcgagaactc atagcccgtg gcgcggtcgc cgccgatgca cacgcccagg acccccggcc
     2641 cgcagccttt gccctgggct tgcagcaccg cgtcgagaat cacccggcgg cacccgtcca
     2701 ggtcgcggct ggctttgagt ttctcgtggg gcagggcgta ttgcgcgcca acattctcgc
     2761 agccgccgcc tttgaggatc agccgcacgc gggtttcggg cgcggcgtgt tgatcaaagt
     2821 ggagggcggg cgagccgggg ccgacgttgg tgccgtcgtt tttgccggtg atggggtcca
     2881 ccgaattctg gcgcagatac cccttttggg tggcccgttt caccgcggcg cgggcggcgc
     2941 gcgcaaagtc aagctggtcg aggccccggg ggcaatccac ataaaacagg atgctgccgg
     3001 tgtcctggca gatgggctgc gatttgtttt tggccagcgc tacatttcgt tggatgatct
     3061 gcattgcgct ggcggcgatc gtgccctgct gttcgcgcgc cagcgatgcc aggagggcct
     3121 ggtggacatc gtcggggatt tcagccgagg tgcggcggat cagttccagc agcgagtctt
     3181 ccagcgcgtt catcgcccgc aggctaagcc gccccgcaaa ccaagtcaat agcgggctat
     3241 ctgtccgtcc gccgaattcc agggcctttg gttcccggcg cctgagtttc ggcggatgag
     3301 ggggaaacgg ttttcccaaa ccccaggccg ctttgcgagt gagtgggcgg gggagaaagc
     3361 gtctagcggt tcaaaacggc cggccgacac tgcgggcaac gcggcgccgc gcacgcgaac
     3421 aggccgaagg gatgaacccc gggggttgga taccctgctg gatcaacagc tcccgtgggg
     3481 cgcggggtgg cgtggttggg gggcgttgcg tcggcgcggt cggaacggac tcgacccggc
     3541 cagcggaatt ccgcgccgcg tcatttcgtc atgaatgtgg gctgaaagtt cgtaattcat
     3601 ttggccgcgc cctccacgat tttgatcttt ccggcgtaag gccgtagacg ccccgcgcgg
     3661 ccgcccgctt ccggttgcgc cagcccggcg cgcccgcgcc gcgagatttt ccacgcccgg
     3721 gcttgctttc ggcaaagact catcttatag ccttgggcgc aatccgacac tccagcaagc
     3781 tatgaacctc tttgcgccgc gaatccttct ccccggcctg gcttccctgg cgttgatctg
     3841 ccccgccatc ccggcgaagg cgcaaacgaa cgccccgccc gccgcctttc ccccaaccgt
     3901 gcccggcgtc gtcattgccc accgcccggc cgcctcgggc atttacatcg gctcgcccag
     3961 cctggccgtt ctgagtaacg gccactatat cgcttcccac gacgaatttg ggcctaaaag
     4021 ctccgagcac cgccgcgcca tctcccatat ctttctatca cgcgaccgcg gcgccacctg
     4081 gggaaaaatc tcgaccctgg acggcgcctt ctggtcctcc ctgttcgtcc atcggaatgc
     4141 gctctatttg ctcgggccgg acaaacacca cggcaacctc ctgattcgcc gttcgctgga
     4201 cggcggcgtt acctggactt cgcccaccaa ccccgcaacc ggactgctgc gcgacgacgg
     4261 ccaataccac tgcgcgccga tgcccgtaat cgaacacgcc gggcgcctct ggcgcgcgat
     4321 ggaacggcga atcccgccca tcgcctgggg catcaactac cgcgccggca tgttttccat
     4381 ccctgtggac gcggacctgc tccaggcaac caactggacc catagcaact tcctgcccag
     4441 cgaccgcgcc tggaatggcg gcgatatggg cgcctggctg gaaggcaacg ccgtcgtctc
     4501 ccccgccggt gaactggtga atgtgctgcg cgtgcaaacc cggtcgcccg aggagaaagc
     4561 cgcccttgtc cacctctctc cggacggccg gacggccacc tttgaccccg ccaccgactt
     4621 catccgcttt cccggcggcg ccaagaagtt cacccttcgc ttcgaccccg aaaccaaacg
     4681 ctactgggcg ctggccagca ttgtccgcga acgccatcgt gctcccgacc ccgggcgcat
     4741 ccgcaatacc ctggccctca ccacctcgac gaacctgctg aactgggagg tgcgctgcat
     4801 cctgctttat catcccgaca ccgccaaaca cggattccaa tacgtggact ggcttttcga
     4861 cggccaggat atcattgccg tctgccgcac cgcctttgac gacggccagg gcggcgccca
     4921 caacaatcac gacgccaact acctgacctt tcaccgcttc gccaatttcc gccgcctcac
     4981 catggcgaat tccgtccctt gaagcgcctc ccgccgtttg cggccgcgcc gagcccccgg
     5041 attccccccg gcgatgcgcg ccgccgcctt gcccgccgcc ttgcgggttc ggccgcgctt
     5101 gcctagcgcc ccagttcctc caagatcaac tttgccgcgc gctggccgga gagcaacatc
     5161 ccgccaaaga tcggccccat ccgcgccgag ccaaacacgc cgttggccgc catgcccgaa
     5221 acgtagagcc cgggataaac ccgccgggtg ttttccaccg tcatcttctc ccccatctcc
     5281 gcccacatcg gcttttcgcc catcaagccc ccggtggggg tttccagccg gatgcccgct
     5341 ttctgcgccg cccgcgcggt gagctccgcc ccgtggcccg tgccgtccaa aaccactttg
     5401 gccctcatgc tcaacggatc cacatgcagg ttgttcagca gaatgggcgt gtagttaatc
     5461 accacgccgc caatccgatc gcccttgaag atgacatctt ccaccgatac cccattgaac
     5521 acctttgccc cggcgtgcac cgcgcgataa atcaggctcg ccgccagttc caccgaatcg
     5581 gcccgcacga accgatcgtc cacgcgggtg tggcggacgc ccaattcctc cagaatcgcc
     5641 acggccgcct gctggatcac cacctcgttg aacagcatcg ccccgcccca caccccgccg
     5701 cccggggcca gtttgcgatc aaacaccgcc acgttcctgc cggcccgcgc gacgtgggcg
     5761 gcgcataaca agcccgacgg ccccgccccc accaccacca aatcgcaatc caaacagctc
     5821 tccagcttgg ccgcgtagcc tcgcacaatc gctttcgaaa tatctacttc cattgaatat
     5881 ctctggtaac attccgggcg cccgctcgca agctcgcagc ccgcgccccc cggcgccgcc
     5941 cgtgcgccgg tctgccgcgg cgaaaaagcc gctgaagctc agggctgaat aacctgcagc
     6001 cgatagaatc tccgctcgtg ggcggtggcg ccaacatcca ggcaggtcag gttggtgccc
     6061 gtgccgttga gtggattcgc ggtgaggtta ttccagacct ggaaattcgt ggaccacagc
     6121 ggctgatact gcacgccgac ggcggagagc cactcgagct gcatgtccgc gccgaggcgc
     6181 gcgagatgca aatagggcgc ggccggcggc gccgggggcg tgatattgcg caggccgata
     6241 ttgcgccgtc ccccccacgt ggcgcccgtg gtgaaggtcc cgcatacacg gctttcccca
     6301 ggcgccaggg tcagcgcgcg attggtccag taatcaacgg gtatggtcag ggccagcgta
     6361 gcggccgtgg tgttcgagag cacgacctgg accgtcgagc tggggacgat ggccccgatg
     6421 ggaatggcga agctgccaac actcgacgag atatcgtagt ggctggtggg ggaaccgtcc
     6481 accaacaggc gaacggccac cccgccgacc gcctccgccg cctcgtaaac accgttgcta
     6541 ttcgcatcgc gaaacagggt atccgtgaag aaataatcaa ccccgcttac gcccaggtcc
     6601 atggtgtaat accggcgata gtccgacgcc tcccaataat aatacccatt tccaatctcg
     6661 cgcaggttga catggtacat attcaaccga tgcccctcgc tgtgccacca gcccatataa
     6721 accgcctccg cgccgacata gccggcggca atgttctcgg ctatcccatt ccagacgtag
     6781 ccttccgcgg taatgcgatc ccaaaaattg gattgcgtca ccgggttata atacgcgctg
     6841 cccaccaccg tttcatgctg gaaaacattc tgtttggcca tgtcctcgct gtgatgccgg
     6901 gccgccaggg ccagcttctg gttgggcgcc agcgggccgg ccgtggccgg cacatccgta
     6961 tagctgctgc cgcgcaactg gttttcactg accgtgtcaa accgcccgcg gttgacccgc
     7021 cagcgaatct cctcctccag ccccgtcggc tccccatcct ccgtgtacaa agtctgggcc
     7081 gcggccggcg ccgggcgccc cagggccagg gcgcaaaata tcaaaaccat ccacggtaaa
     7141 cggttgtttc tcataacaag ctaaccgcta gcatacgaac cgcgcccgcg caagcaaggg
     7201 gtgccgtcgc gacgcccggc tttggccgct gttccatccg ggccgggggg cgggcgccgc
     7261 cgccggaacc gcgcccatca cggagcctcg cgatacggct ggccggcgcg gaaaagctcc
     7321 cgcatccggg cgttgaacgc ggcggcgtcc gcgtcgttgg ccttttccgc aagctcccgg
     7381 gctcgttccg cggcggccac cgcttcggga aagcgtcccg cttcagcgca ggccgccgcc
     7441 agggtccccc acataagcgc ctgccgctga tccgtcaggc ggacggctcg ctccgccagc
     7501 cgaaccgcct ccggcccgtc gcggaattgc gggtcggggt gggtcgccag cgtccaggcc
     7561 aggttgttga gggcctcccc ataacccggc cgcgcggcga gggcggcgcg gtagtgctcg
     7621 atcgcccccc gcgggtcgtg ccgctgtaaa agcaactggg ccaggcgata atgcgcggtt
     7681 gcattccggg gatcgtggcg cagcacctcg gcatactccc gctgcgcctc gtccagccga
     7741 tcccgcacca gccagacgcc ggccaactgc agccgcgccg cggtgttcgt cggctgaagc
     7801 cgcagcagcc cggccagctc gcgcaccgct tcctcgtagt ggccttgatt cgccagcact
     7861 ccgcccagcc cgcagcgggc ttcactcatg acggggtccc gggccagggc ctgccggaac
     7921 gccgcggcgg cttcatccca ttgacgctcc cgcgccagca aggcgcccag gttgctgtac
     7981 ggctcggcgg ccgccgggtc aagccgcgcc gcttcccggc actgggcccg cgcttccgcc
     8041 agccgcccgc tcgcggccag cgccgtcgcc aggccgttgc gcacggtcac gaaccgggag
     8101 tcctggcgca gcgccgcctc ataaaccgcg atggcctcct cattccggtt ctggtcgcgc
     8161 aggacgccgc ccagattccg ccagctcacc tccgactgcg ggcggatctc cagcgccacc
     8221 cggtagcaaa gctccgccgg ccgcagcgcc cgctgcccgg ccaggaacgc cccgaggttg
     8281 ttccaggcca gccagttgct ccgggcgcaa gccagcgtat ggcgaaagag cgtctcggag
     8341 ttctgccaat aaagcaactg cgccccggtg gcgcgcgcgc aggcgatgag cgccgcggcc
     8401 gccgcccccg ccaccaccca ccgtcgccgg cgtcccgccc ccgcaagctc gcccgcgccc
     8461 cacgccgcga tgattaacaa ccccaccgtc ggcagatacg tgtaacggtc cgcaatcgtc
     8521 tggctgccga cctgcaccaa accgatcacc ggcaccagca tgcccagata ccaaagccag
     8581 cccatcagca ggtacggccg cttcttccgc tgcccccacg ccagcagcgt cgcggcggcc
     8641 agcgccccca gcgccgccgc cagcgcggcg gccgacgcaa aggcggggga atcatagtgc
     8701 acgatcaagt ccgccggcca aaccagcttc cccaaatacc gcccatagcc gcccaccgcg
     8761 ttctccagcc gcgcgcccag gggcagcgat tgcagctcca ccaacgcccc tcccgctttc
     8821 tgcgcccaga ccgtcacccc gcaagacgcg gcggacagcg cgaaaaaggg cgccttctcc
     8881 accagcagcc caacccaccg ccgccggccg aaaaccgccg cgccgccgcg cggcgcgttc
     8941 catttcccga tccgccccag cggccaataa tccagcagca gcatcaccaa cggccaggtc
     9001 accagcatcg gcttgctcat caaccccagc gccaaacatc ccaccgacag gagatagcaa
     9061 accaaagccg ctcccttaac cccgcccgcg ccgccgccgg ccggcgcggc gccggcccgc
     9121 tccggctcac gggcccggcg gcgggacgca tacgcggtat aagcccacaa cgacagcaac
     9181 ccgaaaaacg cgctcaacac atccttgcgt tccgccaccc aggccacgga ttcgacccgc
     9241 aggggatgca cggcaaagag cgcggcgacc agcaggctgc gccaaaacgc gccggtgagg
     9301 gaacgaagcc aaagaaacag caggagcgcg ttggccgcat gaaaaagcgc attggtcgcg
     9361 tgatggcccg cgggccgcag cccgtagatt tggcagtcca gcatgtgcga cagccacgtc
     9421 agcggatgcc agttcgccgc atggcaccgg gtgaacgccc agcgcacggc gccgcccgtc
     9481 aacccctgcc gcacctgcgg gttcgccgtg acgtagtcgt catcatccaa attgatgaag
     9541 tccagctcca ccaccggcag gtacaccgcg agcaccgcca gcaccagcaa ggcggcgacc
     9601 caccatgcgc gttcttgatt ccggttttgc gacattcgac cgggaattgt tgggccggcc
     9661 cgcgaaagcg caagccacaa tgcatcccca ttgacttccc gccgcgcccg ccccaaagta
     9721 attcgcatga aaaccaccgc tgcccggcac ttgtctttct cgcgaagttc ggcccttaat
     9781 tccacgggct ttggcgcccg ctccggggcg ctgacgctga cggccgccct gctgctgctc
     9841 tggctcgccg ccgggccggg ctatgccgcc gcgcccgccg ccgccccgga agcccgcgga
     9901 atctgggtcc accttaccca cttcaacccc gacccggacc gcggcaaggc ggaggtcaag
     9961 gcgttcgccg accgctacgc cgatgcccac ttcaatttcg tgctcccctg ggtgaccagc
    10021 gattacatcg ccgcgctgac cgatcccgcc tatcaaacca accagcccac cgcccggtgg
    10081 gatgcgctcg gggaactggt ccgctgcgcc cgcgaacgcc atttgcaggt gcatctctgg
    10141 tattccttca cggattaccg gttcgcgcgc agcccggact tcaacccccg gcacggcggc
    10201 gatcccgcct gggccgcgcg ccgggtaacc ccgcccgccc aagccggcca gtcgccgcag
    10261 gtgacctcgc ccatgaacga tgtctgcccc atgcaccccg aagcccgggc ctggcagttg
    10321 aagcaggtgg actctctgct ggaccgctac ccgggcattt ccatccacat cgaagaaccg
    10381 ggttatggta ccaccgggaa ttgcgcctgc gatctctgcc gttccctgta ccggaaaatc
    10441 cacggcacgg atatcgtgga tgccatcaac agcccggcgg ccgaagattt ccgctgcctg
    10501 ggcaccaccg agttcatccg cgcgctccgg gccaagctgg aaaaacgcaa cccccgcccc
    10561 atcttttccg tcaacggcgg cccctactgg agttccgacc gcaaactcgg acgtgactgg
    10621 aaacgctggg cggaactggg gtggctggat tactacgcct cgcagaatta tacccccaac
    10681 ctggagttct tcacccgcct gacccagacc atcctcaaag acctgcaacc ctgccccgtc
    10741 ttcatcggca tcggggtgaa atggagcggc ggcgaaaccc cgatgccgac cgtcctgcaa
    10801 gaaatcgaac tcgcgcgaaa actcggcgcg gccggcgtca ttcttttcag cgcctcggca
    10861 atcaccgact ccgacctggc cgccctcaaa gcgggaccgt ttcaagcgcc ggccgattat
    10921 ccaagcccgc aacgctaacc gccgcggcca ccctttgcct gcccgtttca tccccggcag
    10981 ggaacagcag ccgggcgccg cgcaagggtc aacagcctgc ccgcgccctt tcttttttcg
    11041 ataggccgca tgcccccccg cagcgcccgg ccgcagcctg ccgcccaata cgtggctcgg
    11101 gaaccttccc accacgcccg gcggaagaac ctgcaccacc cctcga
//
LOCUS       DAOPQG010000115        11056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6769_length_11056_cov_13.962640, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000115 DAOPQG010000000
VERSION     DAOPQG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6769_length_11056_cov_13.962640"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1131
                     /locus_tag="PKI07_11585"
     CDS             139..1131
                     /locus_tag="PKI07_11585"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOC51726.1"
                     /translation="MKHQLDFERPIVELQNKLDELKKHSATHPMNLSVEEEVGMIEKK
                     LEETRRQIFANLSAWDRVKIARHPLRPFTLDYLKAAFQGFSELHGDRRFAEDRAIVGG
                     FATLGTHKVMVIGTQKGRDTKENILRNFGSAHPEGYRKALRLMQLADKFRLPIITLID
                     TAGAYPGIGAEARHIAEAIAVNLREMMVLEVPIIAAVVGEGGSGGALGIGVADRVIIM
                     ENAYYSVISPEGCAAILWKERAAAAKAAEALKITAKHLLELGLVDEVIPEPLGGAHAD
                     PAAAAKNLKEYLLKHLEQLKALSVDDRLKLRYDKFRAFGHFREKPPAPAEAAAT"
     gene            1171..2139
                     /locus_tag="PKI07_11590"
     CDS             1171..2139
                     /locus_tag="PKI07_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOC51727.1"
                     /translation="MLYPLTFQPIFKQLVWGGRELERRYGKVLPPEVPIGESWEISDR
                     PNDVSVIANGPLAGKDLRWLMENCRTELLGNAPSPGGRFPLLLKILDAQTKLSLQVHP
                     PAARAAELGGEPKTEMWYITQAAPGAELFAGLKRGVTRAEFERKIQDGTVADCFHRIS
                     ARAGDALFLPSGRVHGLGAGLVLFEIQQNSDTTYRVFDWNRVGLDGKPRPLHIPQALA
                     SIDFNDFEPELLPAAWSPGETCQVRPLVRESVFAVEACAAAAGARVPLPPRKMQIVAM
                     LAGRARIGDGSEALALAAGQFGLVPASLPPMSVRAAEPVTFLRIEL"
     gene            2136..2567
                     /locus_tag="PKI07_11595"
     CDS             2136..2567
                     /locus_tag="PKI07_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOC51728.1"
                     /translation="MKKFLKGWAINTLAVLVAVYIVPGLRFTDNSLLTPLVTSLVLGI
                     LNAFIRPVLLLLALPLLIYTLGLFTLVINALLLYLVSYLLAQHFAVDSFGAALAGALI
                     ISIVSLVLNLLTGTSRARVRYQRGRQPPDANNRGQGPVIDV"
     gene            complement(2594..3178)
                     /gene="trmB"
                     /locus_tag="PKI07_11600"
     CDS             complement(2594..3178)
                     /gene="trmB"
                     /locus_tag="PKI07_11600"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOC51729.1"
                     /translation="MSLIYALPSIVERLDLAKLFERAQPLEVELGSGDGSFLAEYARQ
                     NPEHNFIGVERLLGRLRKLDRKGQRAGLTNLRGVRLECAYFVQYLLPPGSVRALHIYF
                     PDPWPKRKHRRRRLVNERFPALARQALQPGGRVYLRTDDPDYFEQMVRVFGAEPAFHS
                     VDTPAELAAQLTDFEREFLARGIKTRHAAYQAGS"
     gene            complement(3207..4649)
                     /gene="ahcY"
                     /locus_tag="PKI07_11605"
     CDS             complement(3207..4649)
                     /gene="ahcY"
                     /locus_tag="PKI07_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOC51730.1"
                     /translation="MATLTRNRSKQRTVREGRIKDPGLADWGRKEITVAEQEMPGLMA
                     VREKYGASKPLKGVRVTGSLHMTIETAVLIETLVELGAAVRWASCNIFSTQDQAAAAI
                     AQAGVPVFAFKGESLEDYWDFTLAALTHPGGRGPELVVDDGGDATLLLHRGYEMEHGS
                     DWANTPSDSHEEQVIKNLLRRCAKERPGWFTRAVRDWKGVSEETTTGVHRLYQMLAAG
                     KLLVPAINVNDSVTKSKFDNLYGCRESLADGLKRALGVMIAGKTACVCGYGDVGKGSA
                     HSLRGFGARVIVTEIDPINALQAAMEGFEVKTVEDTLGEADIYVTCTGNRDVIRLEHI
                     LKMRDQAIVCNIGHFDNEIQVERLNAARGVVRVNIKPQVDRYDLPGDRSIYLLAEGRL
                     VNLGCAEGHPSFVMSNSFTNQVLAQLDLWNNRATYKPAVYRLSKKLDEEVARLHLGKL
                     GVKLTRLTRQQADYIGVPIEGPYKPEHYRY"
     gene            complement(4701..5948)
                     /gene="metK"
                     /locus_tag="PKI07_11610"
     CDS             complement(4701..5948)
                     /gene="metK"
                     /locus_tag="PKI07_11610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOC51731.1"
                     /translation="MSQNYIFSSESVGEGHPDKVCDTISDAVLDACLAQDPRSRVACE
                     TYAKSNLVVVGGEITTRARLDFNAIARQTIRDIGYTHADDVFHADRVMILNAVTSQSP
                     DIAQGVDARSAEGKGTAEQGAGDQGLMFGYATKETPELMPAPIMFAHQLGRELTRIRK
                     TGAIKWLRPDAKSQVSVQYVNGQPARITNVVVSTQHAADVKHRTIESFIIKEVVKKVL
                     PPHLLDRQTTYLVNPTGRFVVGGPQGDTGLTGRKIIVDSYGGVGRHGGGCFSGKDPSK
                     VDRSAAYMGRYVAKNIVAAGLATSAEIQFAYAIGHPEPVSVCINTFGTGVVSDEELER
                     AVAEVFSFKPADIIRQLRLLRPIYLKTTNYGHFGKNDPDITWEKTDQAAALRRAASRG
                     APAVAAATLKKAAARLKRRAGRA"
     gene            6046..6975
                     /locus_tag="PKI07_11615"
     CDS             6046..6975
                     /locus_tag="PKI07_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC51732.1"
                     /translation="MSATLKCLRALSDPTRLRIVALLERDELSVNELQEITRMGQSRI
                     STHLGLLQDSGFVQSRREGKRAFYRLGPAAGTMAGELRQLAIRGAGELPGHADDQVNL
                     KRILARRRAQAQVYFNQVAGRFDRVYGPGRSWEAFGHLLLRILPPLVVADLGAGEGLL
                     SELLARRCRKVIAVDNSRKIVAFGAAKARRNNMKNLEFRLGDLQDPPVAPASVDLAIL
                     SQALHHAQDPARAIASAARILKPGGQIMILDLARHRFERARKLYGDHWLGFAESELHR
                     WLEAAGFKHIEISRVAREEAPPHFETLLAAAQK"
     gene            7247..7618
                     /locus_tag="PKI07_11620"
     CDS             7247..7618
                     /locus_tag="PKI07_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51733.1"
                     /translation="MKRTASHIGGFTLVEIMIVVAIIGLLASIAVPNFTKARMTARMN
                     ACISNLRVIDGAIQQWALDTKQGETAQVTAADILPYLKNAVTCPSGGKTFTDSYSVTT
                     VEARPTCLIQPSTHKLEETLQ"
     gene            7843..>8115
                     /locus_tag="PKI07_11625"
     CDS             7843..>8115
                     /locus_tag="PKI07_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOC51734.1"
                     /translation="MKNMTSRKSGFTLVEIMIVVAIIGLLAAIAIPNFVRARTQSQKN
                     ACINNLRQIDGAAQQLALENKLAASATVTTADLIPYLKAAPVCPAGG"
     assembly_gap    8116..8215
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8693..9448
                     /locus_tag="PKI07_11630"
     CDS             8693..9448
                     /locus_tag="PKI07_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC51735.1"
                     /translation="MEPIIETRNLRVEFRAQNSGSEKKVAVKDLNLRICGGEVFGFLG
                     PNGAGKTTTMNVLLGFVNATRGSACLFGVDVREPIARQRIGYLPELTYYYKFLTAEEL
                     LRFYARIFGLPRAVADQRIEQLLKLVELDTARRRLIKTYSKGMQQRVGLAQALINNPD
                     LLILDEPTSGLDPLGRMKVREIIQRLKNEGKTVFFSSHELGEVETVCDRVAIINQGEL
                     KVEGRVADLVREHQANLERIFLNIIGYQPESDA"
     gene            9445..10224
                     /locus_tag="PKI07_11635"
     CDS             9445..10224
                     /locus_tag="PKI07_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOC51736.1"
                     /translation="MNTIFALANVVIKELCRRKDFYVLFVLTAVLTLLLGSVSFFQDI
                     KVVRYVKEIALLLIWISALVIAIATTARQLPAEREHRTIFPLLAKPVTRGQVILGKFV
                     GCWLACGLALVVFYLFFGVVSGSREHSWPVLNWLQAFWLQWSMLGIVVALVLLGSIVF
                     AAPSSNATICFILVLGILLLGRHLNQVALQQPEPLRSVVYGLYFFIPHLEWYDVRDLI
                     IYDHQLIGWGDCALASLYAGVYAALLLFGAWLLFRRKPLHL"
     gene            10221..>11056
                     /locus_tag="PKI07_11640"
     CDS             10221..>11056
                     /locus_tag="PKI07_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51737.1"
                     /translation="MKRLVPYLILGLLAVGCFSLATVLQARAYGWSRRAQADNILKVL
                     LGDGRRLFANHFFIQADVSFHSGYYPSIFDQARAAQGSRHLTAEEGDPAHEEHEKSMD
                     FLGPPRDWIERFGRHFMVTDHTHLHGDTESEILPWLKISAELDPQKVETYTVAAYWLR
                     KLNKASEAEEFLREGLRNNPNSHEILFELGRLCAEYHHDPARARKMWELGLRKWADQE
                     AAGQKPELFHLDQLAVHLARLEEQEGNLARAIELLQLAIKATPHPEALQRQIEELEQR
                     RAA"
BASE COUNT         1987 a         3458 c         3397 g         2114 t
ORIGIN      
        1 ttggccgcat tacccttctg agcgtctggg tgaaggcgta taaacctcgt caccacgaag
       61 agccggatgc aaatccggct ctttgcttgt ttgaaatgag cgttgcgcgg gcggaaaaag
      121 gccactaact tgggcgcgat gaaacaccaa ctcgactttg aacggccgat cgtggagttg
      181 caaaacaagc tcgacgagtt gaaaaaacac tccgcgactc acccgatgaa cctcagcgtc
      241 gaggaggaag tcgggatgat cgagaaaaag ctcgaggaaa cccgccgcca gatatttgcc
      301 aatctgagcg cctgggaccg ggtcaaaatc gcccggcacc cgttgcggcc ctttacgctg
      361 gattacctca aggcggcctt tcaggggttc agcgaactgc acggcgaccg ccggtttgcg
      421 gaagatcgcg ccatcgtggg cgggtttgcc accctgggca cgcataaagt catggtgata
      481 ggcacgcaaa agggtcggga caccaaggaa aacatcctcc gcaacttcgg ttcggcgcac
      541 ccggaaggtt atcgcaaggc gctccgattg atgcagttag cggataagtt ccgactgccg
      601 atcatcacgt tgattgatac cgccggggcc tacccgggaa taggcgccga ggcgcgccat
      661 atcgcggagg ccatcgcggt gaatctgcgc gagatgatgg ttctggaagt gccgattatt
      721 gcggccgttg tcggggaagg gggctcgggc ggggcgctgg gcattggggt ggcggaccgg
      781 gtgattatca tggaaaacgc gtattattcg gttatcagcc cggaaggctg cgcggcgatc
      841 ctttggaaag aacgtgccgc cgcggccaaa gccgccgagg cgctcaaaat tacggccaaa
      901 cacctgctcg aattggggct ggtggacgaa gtcatccccg aaccgctggg cggtgcccat
      961 gccgaccctg ctgcggcggc caaaaacctg aaggaatacc tgttgaagca tctggaacag
     1021 cttaaagcct tgtcagtgga tgaccggctc aagctgcgct atgacaagtt ccgcgcgttt
     1081 ggccatttcc gcgagaaacc acccgcaccc gccgaggccg cagctacttg atgcccgtgg
     1141 tcagccaggt tctcaaccct tgaaaccagc atgctctacc cgttaacctt ccagcccatc
     1201 ttcaagcagc tcgtctgggg cgggcgcgaa ctggaacggc ggtatggcaa agtgctgccc
     1261 ccggaggtgc ccatcggcga gtcgtgggaa atcagcgatc ggcccaacga tgtcagcgtc
     1321 atcgccaacg ggccgctggc cggcaaagac ctccgctggc tgatggagaa ttgccgaacg
     1381 gaactgctgg gaaacgcgcc gtctccgggc ggacgattcc cgctgctgct caagatcctg
     1441 gacgcgcaaa cgaaactttc cctgcaagtg catcctccgg ccgcccgggc agcggagctt
     1501 ggcggagagc ccaagaccga gatgtggtat atcacgcagg ccgccccggg cgcggagctg
     1561 ttcgccggtc tgaaacgcgg agtcacccgc gcggagtttg aacgcaaaat ccaggatggc
     1621 acggtggccg actgctttca ccggatcagc gcgcgggcgg gagacgccct gttcctgccc
     1681 agcgggcgcg tgcatgggct tggtgctggc ctggtcctct ttgagattca gcaaaactcg
     1741 gacaccacct accgggtctt tgattggaac cgggtgggcc tggacggcaa gcctcgcccg
     1801 ctgcatatcc cgcaagccct ggccagcatt gactttaacg atttcgagcc ggaattgctc
     1861 ccggcggcat ggagcccggg cgaaacgtgc caggtgcggc cgctggtgcg cgaatccgtt
     1921 ttcgccgtcg aagcctgcgc tgccgccgcc ggtgcgcgcg tgccgctccc gccgcggaaa
     1981 atgcagattg tggcgatgct ggccgggcgg gccaggatcg gcgacggctc cgaggcgctt
     2041 gccctcgcgg cggggcaatt cggcctcgtg ccggcgagcc tgccgccgat gagcgtgcgc
     2101 gccgccgagc cggtcacctt cctgcgcatc gagctatgaa gaagtttctc aaaggctggg
     2161 cgatcaacac gctcgcggtc ctggtggcgg tttatatcgt cccgggcctg cgcttcaccg
     2221 acaacagctt gttgaccccg ctggtcactt cgcttgttct gggcattctg aacgccttca
     2281 tccggcccgt gctcctgttg ctggctctgc cgctgctgat ctatacgctg ggcctcttca
     2341 cgctcgtaat caacgcgctg ctgctctatt tggtgagcta tctgctggcg cagcacttcg
     2401 cggtggacag cttcggagcg gccctcgcgg gcgcgttgat tatcagcatc gtttccctgg
     2461 tgctgaacct cctgaccggc accagccggg cccgggtccg ctatcagcgc gggcgccagc
     2521 cacccgacgc aaataaccgc ggccaggggc ccgtcattga cgtctgaatc ccgtccgcac
     2581 tccgccttgg ccgtcaagac ccagcctggt aagccgcgtg ccgggtttta attccgcgcg
     2641 ccagaaactc ccgctcaaaa tccgttagct gggcagccaa ttcagcggga gtgtccacgg
     2701 agtggaaagc cggctccgcg ccaaacacgc gcaccatttg ctcgaagtaa tccgggtcat
     2761 cggttcgcaa atacacccgg cctccgggct ggagcgcctg ccgggccagg gcgggaaagc
     2821 gctcgttgac gagccggcgg cggcggtgct tccgtttggg ccacggatcg ggaaaataga
     2881 tgtgcagcgc ccgcacggaa ccgggcggca acaggtattg gacaaaataa gcgcattcca
     2941 ggcgcacccc gcgcaggttc gtcagtccgg cgcgctggcc tttgcggtcg agttttcgca
     3001 ggcgcccgag caaccgctcc accccgatga agttgtgctc cgggttttgg cgggcatact
     3061 cggccaggaa cgacccgtcg ccgctgccca gttccacctc caacggctgc gcccgctcga
     3121 acagctttgc caggtcaagc cgctcgacaa tggacggcag cgcgtagatc aggctcattc
     3181 ccgcacccgg ccggtgcgcc ccaacgctaa tagcggtagt gttccggctt gtaggggccc
     3241 tcgatgggga cgccgatgta gtcggcctgc tggcgggtca ggcgggtgag cttgacgcca
     3301 agcttgccca ggtgcaggcg ggcgacttcc tcatcgagct tcttggagag acgatagacg
     3361 gccggcttat aggtggcgcg attgttccac agatcgagct gggccagcac ctggttggta
     3421 aacgagttgg acattacgaa gctcggatgc ccttcggcgc agcccaggtt caccagccgc
     3481 ccctcggcca gcaggtaaat gctgcggtcg cccggcaggt cgtagcggtc cacctgcggc
     3541 ttgatattca cccgcacgac gccccgggcg gcattcaggc gctccacctg gatctcattg
     3601 tcgaagtggc cgatattgca gacgatagcc tggtcccgca tcttgagaat atgctccagc
     3661 cggatcacat cgcggttgcc ggtgcaggtg acgtagatgt ccgcttcgcc cagcgtgtcc
     3721 tcgacggtct taacctcgaa cccctccatc gcggcctgca aggcgttgat cgggtcaatc
     3781 tcggtcacga ttacgcgcgc cccgaacccg cgcaggctgt gggcgctgcc tttgccgaca
     3841 tcgccgtagc cgcagacgca ggcggttttg ccggcgatca tcacgcccag ggcgcgcttg
     3901 aggccgtcgg ccagcgattc gcggcagccg tacaggttgt cgaacttgga cttggtgacg
     3961 gagtcattga cgttgatggc gggcaccagc agtttgccgg cggcgagcat ctggtagagg
     4021 cggtggacgc cggtggtggt ttcctcgctc acccctttcc agtcccggac cgcccgggtg
     4081 aaccagccgg ggcgttcctt cgcgcagcgc cgaagcaggt tcttgatgac ctgctcttcg
     4141 tggctgtcgc tcggggtgtt ggcccaatcg ctgccgtgct ccatctcgta gccccggtgc
     4201 agcagcagcg tggcatcgcc gccgtcgtcc acgaccagtt ccggcccccg gccgccggga
     4261 tgcgtcaacg ccgccagggt gaagtcccag tagtcctcca gcgattcgcc tttgaacgcg
     4321 aagacgggaa cccccgcctg ggcgatcgcc gccgccgcct ggtcctgggt gctgaaaata
     4381 ttgcaactgg cccagcgcac cgccgcgccc agttcgacca gcgtctcgat cagcacggcc
     4441 gtctcgatgg tcatatgcag cgagccggtc acgcgcacgc ccttcagggg cttgctcgcg
     4501 ccatacttct cgcgcaccgc catcagcccc ggcatctcct gttccgccac ggtgatttcc
     4561 ttgcggcccc aatcggcgag gccggggtct ttgatccggc cctcgcggac cgtgcgttgt
     4621 ttgctcctgt tcctggtcaa agttgccata atgcttgtgg ttgaggttcc gggggttgac
     4681 tcaaaggctt ccgccccagc tcaggcgcgg ccggcccggc gcttgagccg cgccgcggct
     4741 ttcttcaagg tggccgccgc caccgccggc gcgccccggc tcgccgcccg gcgcagagcc
     4801 gccgcctggt ccgtcttttc ccaggtgatg tccgggtcgt tcttgccgaa atggccgtag
     4861 ttggtggtct tgagataaat gggccgcagc aggcggagct gccggatgat gtccgccggc
     4921 ttgaagctga acacctcggc taccgcgcgc tccagttctt cgtcgctcac cacgcccgtg
     4981 ccgaacgtgt tgatgcaaac cgacaccggc tccggatgcc cgatggcata ggcaaattga
     5041 atctcggcgc tcgtcgccag cccggccgcc acgatattct tggccacata ccggcccatg
     5101 taggccgcgc tgcggtccac tttcgacggg tccttgccgc tgaagcagcc gccgccatgc
     5161 cggcccaccc cgccgtagct gtccacgatg attttgcgcc cggtcaaccc cgtgtcgccc
     5221 tgcggcccgc ccaccacaaa ccgcccggtc gggttgacga gataggtcgt ctgccggtcg
     5281 agcaggtgcg gcggcagcac cttcttcacc acttccttga tgatgaaact ctcgatggtc
     5341 cggtgcttca cgtcggcggc gtgctgcgtg gacaccacga cgttggtgat gcgcgccggc
     5401 tggccgttga catactgcac cgagacctgg ctcttggcgt ccgggcgcag ccatttgatc
     5461 gcgccggtct tgcgaatgcg ggtcagttcg cgccccagtt gatgggcgaa catgatcggc
     5521 gccggcatca attcgggggt ttccttcgtg gcgtagccaa acatcaggcc ctggtcgccc
     5581 gccccctgct cggcggtccc ttttccttcg gcggagcggg catccacgcc ctgcgcaatg
     5641 tccgggcttt gcgacgtgac cgcgttcaaa atcatcaccc ggtccgcgtg gaaaacgtca
     5701 tccgcatgcg tatagccaat gtcgcgaatg gtctggcggg cgatggcgtt gaagtccagc
     5761 cgggcccggg tggtgatttc tccgcccacc accaccaggt tgctcttggc gtaagtctcg
     5821 caggccaccc ggctgcgcgg gtcctgggcc aggcaggcgt ccagcaccgc atccgaaatc
     5881 gtgtcgcaca ccttgtccgg atggccttcg ccgacggatt cagaggaaaa aatgtaattc
     5941 tggctcatag tatcaagcgg tatccacagg tcttcaattc atattgacgt atcaggataa
     6001 catgatacat tcaggtatgc ctgtcaaccg gtttctcgcc cgcccatgtc cgccaccttg
     6061 aaatgcctcc gggccctgtc cgaccccacc cgcctgcgga tcgtcgcgtt gctggagcgg
     6121 gacgagctct ccgtcaacga actgcaggaa atcacccgga tgggccagtc gcgcatttcc
     6181 acccacctgg ggctgctgca ggattccggc tttgtgcaat cccggcggga aggcaagcgc
     6241 gccttttacc ggctgggccc ggccgccggc accatggccg gcgagctccg ccaactggcg
     6301 atccgcggcg ccggggaact gccggggcac gcggacgacc aggtcaattt gaagcgcatc
     6361 ctggcccgcc ggcgcgcgca ggcgcaggtt tatttcaacc aggtggcggg gcggttcgac
     6421 cgggtctatg gcccggggcg ttcctgggag gctttcgggc acttgctgct gcgcatcctg
     6481 ccgccgctgg tggtggcgga cctgggggcg ggcgagggct tgctgagcga gctgctggcc
     6541 cggcgctgcc gcaaagtcat cgcggttgac aactcccgga agattgtcgc cttcggcgcg
     6601 gccaaagccc ggagaaacaa catgaagaac ctcgaatttc gcctcggaga cctccaggac
     6661 ccgcccgtgg cgccggccag cgtggatttg gcgatcctca gccaggccct gcaccacgcg
     6721 caagacccgg cccgggcgat tgccagcgcc gcccgcatcc tgaaaccggg cggccagatc
     6781 atgattctcg acctcgcccg gcatcgcttc gagcgcgcgc gcaagctcta cggcgatcac
     6841 tggctggggt ttgccgaaag cgaactgcac cgctggctcg aagccgccgg cttcaaacac
     6901 attgaaatca gcagggtcgc ccgcgaagaa gcaccgccgc actttgaaac gctcctggcg
     6961 gcggcgcaaa aataacgtgc gcggcgcgat ccggccgcct ccttttcgac ccggcctatc
     7021 cacttttgag acactgtccc gggacactgc ccatttcttc atccccggcg ccagccgcgc
     7081 ctcgggtcaa aaacggactc gccgcgacaa agcccggggt tgactccctt cgccaaagag
     7141 gtaatttaca gggtgcggtg agcaaccggc aatacgtgaa caaatactgt aatcataata
     7201 accgccggtg gctctgttca cgccgcaatg atagaaagac taaatcatga aaagaacagc
     7261 atcccacatc gggggcttta cgctggtgga gatcatgatc gtggtggcca ttatcggcct
     7321 gctcgcctcg atcgcggtcc ccaacttcac gaaagcccgt atgacggctc gtatgaacgc
     7381 ttgtatcagc aacttacgag taattgacgg ggcgattcag caatgggccc tggataccaa
     7441 gcagggggaa acggcccagg tgaccgccgc ggatatcctg ccgtacctga agaatgcggt
     7501 cacatgcccc tccggcggca agacattcac cgacagctac tcggtgacga ccgtggaggc
     7561 ccggcccacg tgcctcattc agcccagcac tcacaaactc gaagagacgc tgcagtagcc
     7621 gccggttctt gcaattcagt tcgaccggcc cgggaatgat cccgggccgc tttttttaac
     7681 cgggtcaaag ggaggggata aggagccttc cccgggttgg catccggtat gctgaaccct
     7741 tcaagtgcgg ttgcagcggg caatcagtga acagaaaccg tactaataat aacccgcccg
     7801 ttgcgtgttc acgccgcaag aacagaaaga ccgaaagcaa gtatgaagaa catgacctcc
     7861 cggaaatcgg gcttcaccct ggtggaaatc atgatcgttg tggccatcat cggcctgttg
     7921 gcggcgatcg ctattcccaa cttcgtgcgg gcccgcacac aatcgcagaa gaatgcctgc
     7981 atcaacaacc tgcgccagat tgacggcgcc gcgcagcagc ttgccctcga aaacaagctg
     8041 gcggcttcgg caaccgtcac cacggcagac ctcattccgt atctgaaggc tgccccggtg
     8101 tgccctgctg gcggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggatt
     8221 ttaccttgta tctccggtga gcatcccttg tacacccctt gtacacggcc tataattggc
     8281 tataaacagg ctcgaaatgg cctggatcgc gcgaatctgg ggataacgca gactgacagg
     8341 gaaggggagg tgccaagccg gctatttcca tgagtgtcca aaaatgggac actcctgaag
     8401 gccgtatggc tcgcgtagag gcgattgctg tttggttggg gaaagacttg ttgagagggc
     8461 ttcgagctaa aatgcaggca tttttatccg cataaaggct gaaagtaaga agagtggata
     8521 ggagtatccg ggcttcaggc ggcgttctga accgcggcgc aaggacgcag gcgggggtta
     8581 tgaggtactg attttgctgg tttatggtag agattttgct tgagttggaa agtggttgtc
     8641 tcgtgcccgg cgcacactat gctgagcatg aaaacgagag cggccaggaa ccatggaacc
     8701 gattattgaa accagaaacc tgcgcgtcga gtttcgcgca cagaactccg ggtcggagaa
     8761 gaaggtcgcg gtcaaagacc tgaacctgcg catttgcggc ggcgaagtat tcggcttcct
     8821 cggccccaac ggcgcgggga agacgacgac catgaacgtg ctgctggggt tcgttaacgc
     8881 gacccgcgga tcggcctgcc ttttcggggt ggatgtgcgc gagccgatcg cccgccagcg
     8941 cattggctac ctgcccgagc tgacgtatta ctacaagttc ctgaccgccg aggagttgct
     9001 gcgattctac gcgcgcattt ttggccttcc gcgggccgtg gcggatcagc gcattgagca
     9061 gcttctcaag ctggtcgagc tggacaccgc ccggcggcgg ctcatcaaga cctattccaa
     9121 ggggatgcag cagcgggtgg ggctggccca ggccctgatc aacaatcccg atctgctaat
     9181 cctggatgag cccaccagcg ggctggaccc gttgggccgc atgaaggtgc gggagattat
     9241 ccagcggctg aagaacgagg gcaagacggt ctttttctcc tcccatgagc tgggcgaggt
     9301 cgagaccgtc tgcgaccggg ttgccattat caaccagggc gaactcaagg tcgaggggcg
     9361 cgtggccgac ctggtcaggg aacaccaggc caacctcgag cggattttcc tgaacattat
     9421 cggctatcaa cctgaatccg acgcatgaat accatcttcg cgctcgctaa cgtggtcatt
     9481 aaggaactgt gccggcgcaa ggacttttac gttctgttcg tcctgaccgc cgtcctgacc
     9541 ctgctgctgg ggtcggtgag ttttttccag gatatcaagg tggtccgcta cgtgaaggag
     9601 atagccttgc tgctgatctg gatttcggcg ctggtgatcg ccattgccac caccgcccgc
     9661 cagctcccgg ccgagcgcga gcaccgcacg atctttcctt tgctggccaa gccggtgacg
     9721 cgcgggcagg tgattcttgg caagtttgtc ggctgctggc tggcgtgtgg cctggcgctg
     9781 gtggttttct atcttttctt cggagtggtc agcggctcgc gcgaacattc ctggccggtg
     9841 ctgaattggc tgcaggcctt ctggctgcaa tggtccatgc tgggcattgt ggtggcgctg
     9901 gtgctgctgg gctcgatcgt cttcgccgcc ccgtcgtcca atgccaccat ctgctttatt
     9961 ctcgtgctcg ggattctgct cctggggcgc catctgaacc aggtcgcctt gcagcagccc
    10021 gagccgttgc gttcggtcgt gtatgggctc tactttttca tcccgcacct cgaatggtat
    10081 gacgtgcgcg atctcattat ctacgaccat cagctaatcg gctgggggga ttgcgccctg
    10141 gcgtcgctct acgccggggt ctatgcggcg ctgctcctgt tcggcgcctg gctgttgttc
    10201 cggcgcaagc ctttgcacct gtgaaacgcc tggttccgta tctgattctg ggcttgctgg
    10261 cggtggggtg cttcagcctg gccacggtgc tgcaggcgcg cgcctatggc tggagccggc
    10321 gggcccaggc ggacaacatc ctgaaggtgc tgctcgggga cggccgccgt ctgtttgcca
    10381 atcatttctt catccaggcc gatgtctcct ttcatagcgg gtattacccg tccatcttcg
    10441 accaggcccg cgccgcgcaa ggctcccgcc atctcaccgc cgaagagggg gatcccgcgc
    10501 acgaggagca tgaaaaaagc atggacttcc ttggcccgcc ccgggactgg atcgagcgct
    10561 tcggccggca tttcatggtc accgatcaca cgcacctgca cggggatacc gaaagcgaaa
    10621 tcctgccctg gctcaaaatc tccgccgagc tggacccgca gaaggtcgag acgtacaccg
    10681 tggccgccta ctggctgcgc aagctgaaca aagcctctga ggcggaggag ttcctgcgcg
    10741 agggcttgcg caacaacccg aacagccatg aaatcctttt cgaactgggc cgcctttgcg
    10801 ccgagtatca tcacgacccc gcccgcgccc gcaaaatgtg ggaattgggc ttgcgcaaat
    10861 gggccgacca ggaagccgcc ggccagaaac ccgagctttt ccatctcgat cagctcgcgg
    10921 tgcatctcgc gcgcctcgaa gagcaggagg ggaacctggc ccgtgccatc gaattgctgc
    10981 aactggccat caaagccact cctcacccgg aggcgttgca acgccaaatc gaggaactcg
    11041 agcagcgccg cgccgc
//
LOCUS       DAOPQG010000116        11050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6773_length_11050_cov_16.959891, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000116 DAOPQG010000000
VERSION     DAOPQG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6773_length_11050_cov_16.959891"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..215
                     /locus_tag="PKI07_11645"
     CDS             39..215
                     /locus_tag="PKI07_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51738.1"
                     /translation="MRVSIMQIYRAPRRSKWVRPDRSVRQIGVVARLGSRGGKEFTND
                     HRKRITPKQNYEDC"
     gene            202..4764
                     /locus_tag="PKI07_11650"
     CDS             202..4764
                     /locus_tag="PKI07_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51739.1"
                     /translation="MKTVDHCSVPSVVQSKSGANQSPSTEINEDKMTPTLPPRAIIAS
                     LIWLCLAFLNSAMVSGQTATPGANPEESGIRWLLDPGRSPVQVAHQIQCTSGNSNLWE
                     GIVTNTTDPFLVFPPVRIDADSEKILYLNMAVETGRRVQLFFSADDSFQEELSCHARG
                     LAAGSELSLYALDLSEFPKWRGKITSLRLDLDGSRNGTRVKFARIGSGKSGITAGAVP
                     LEVETAATPCTANRRRNAAFQDTIVATDECYGSSAVMTFVKTHFSYVELNVLARLLVR
                     AEAEGRQFEAATAARGLKVEDLPGGVRAQYQLAGAQITSEILPLRIGRDTPGQDGAAL
                     FTIRTEPPVPVIVDCGDSMVENPFGGLFAHSEPTLLNSTDQITLESGTAFLASKAHPL
                     KVAIRGDAALTVKTNLNGGQYLSFRAPSGQLSVLVSYAEDDARLRAIAATDFAQGRKQ
                     VEDYCAELLQARVQTPVKNIDAAFRTALYNLEYAWNAPYGWTESINFWHALWHMQHTA
                     GEEWIGHADRSRLSNETHAENLMPNGAVPQMMQYAHKRRDFGGSNQFFAWQVRHYWHF
                     TGDLEFARKLAPVLDRVIAQTFRENDPDGNYLLGWGQQIGNQEDFISTPHDGTSPAIE
                     GINMFRTRRELALALGDHETAARCEARIHQIQAGLWEKLWLRDLGRFAFYRDPLGELR
                     LDGQYHSMLYPVIWQIADPLDSYSMLRQVRDRLTGKDGEVFCSDNFVTHAVTTTGVQA
                     GIAQQPWAAWALATAGRRNEVVRPLQAAANWVMDDNHRGAWPEISYEPSPAYFSPPIG
                     LWVAATVEALFGLKVDKPAGRLQVAPCFPDHWPAASLNLPDYQAEFTRQGDTLEYVVR
                     SREPLTRQVRWSLPPGRVSDVSVDGKPVEYHLEPGVECLFLALETKPSRASRIRVKVQ
                     PFNLAVNAPASIAEGDPITVSVNGIAIEGVEDHCGLLAETTLASSQQLQGRISTGLLE
                     AYRGYGRLGQMTFARRTFFLRCLGPDQTKYWLPVDLAILPRYEAAPAANLHLDGDTIS
                     AKLLIRNNTVLALNGTAWLQIARDEFPCAVSIAPRSQAEVSVSIPARQAAWLSPGDNA
                     ALLVLPGKATVNLVLNGSPLFREQEPLRQYQKARIKAIALPRENLIPDTQWNTLRSFK
                     AYVHPPWSGLQPPLLALGNQTEVTAGGLEEVRFQLAPRQFVPISHASGRPSWSLSLSN
                     LPCKKLYLLVIPFTDNHDVYSPVAQVAVETAEGAVLARTLRVPGDLDWWAPPAIVGEF
                     ASARQPRKDRFGLLPLLNPAQGDWEEGQPPAFPQSAYWATCRQLHLSSAVLNVVEMDL
                     GRPRLLKSLTVSTLGSDPALGVVALAAETDQGLEALDGTPYAPPAKFAAGRVLFAFQQ
                     PDDTLGWQLEGDAFSVSADPQLFPSFPTLNSVARAGEKATGRATSPEFTIRPHEHWML
                     LEMHGGRSASPGGDCNLCVRLVDAQSGAVLREVLPTGSHLIAEERVGLEGLQGKRVRL
                     ELVDRNSAPTYAWIGLRRVTLTGNEL"
     gene            4761..7958
                     /locus_tag="PKI07_11655"
     CDS             4761..7958
                     /locus_tag="PKI07_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51740.1"
                     /translation="MNRPKSRNGIVRRPSPGVQTRRFIAAALLALGLACWPRPCPAQP
                     AATNGMAATLAFAAGTLEGWVVEGENTWRIGTNPPLFTSPADPIRFVVNSYEGGERNR
                     GVLRSPPFVIEHERQRFSIAGWDGTTNGVNDAMLNFVLLRSHPDGEILRRTHTPGGNK
                     LTPASWQTTDLIGRRVFLEVVDANPVLRPDGGYAWIAFADYRQESAGLFTKPVRRDDL
                     YGLRIDANSERILCRTVPFFAAPPTQRMRTTRSVQGHRETIPVGARAEALCLLGMINH
                     GWEAGTAHWGEHPELRPKRDDQLYVGANIGEIELCYAGGARDRVPLVIGATAWFAGFW
                     GYSQFSDVRVLPREPFASRPEYMAVLQRALRLRQSEEPGSAENAHALYFLTVKPRPQK
                     IESIVVHDNPANRGQPLVTAITLVGAKPATNLLHFGTCRLEAADLAPAFSTARPGDWS
                     RDLAALARVLYTRDADLPRKVAPVEVPADFKAARIRFRGGPLADMLSNIWLANLYQIS
                     QKFERGSGFFHETETNSPWYGSYSGIGTWAHLGVYAPYAYSRCSDHFASLALRCIDDP
                     VRVASYMDFVDYWFYWHRDNHDPDQGPPNDVLDVARYPKGVTGHWSFGLPPGLPPWQL
                     NEIPGVEEMDGHGATMVARWLAWRVLGARTDQWLTGARDNVFGKSRWDTTRDAAEFVC
                     WLMDHTGMDVIYCEGETTGWAGGPQVPLTGAPDWWQETDPVKLRKNYANANAYEVYPT
                     YVCLTALRCSAQIADALPDAALAAKWRRYADRLESGMLRLLAVGEFGSLTWRLSRHSV
                     YPSGQDSLVQAWFAFYFDGLDPNRLPPQATGITRNTLRRQLSLPCGRAPALAMGYGLG
                     WLCKAALVLDEMDEAGPMLLNIARYTYDKNMDYADPSRGIDWRRFAWIVPEGANILPD
                     GSWHRIGDLSNGANQGPPLHAMEICAGFDDTKPNDLRLLPRVPNPLTGIEVEDFMVLV
                     PDGAGLARAKVSYAYNRAAASFRLRSDRPLPRLALRLGPFHAAEARRAAQTLKSSAKT
                     DVRTESSGTFQAQAAWWVWLENLKDIASLDLDWKS"
     gene            7962..10325
                     /locus_tag="PKI07_11660"
     CDS             7962..10325
                     /locus_tag="PKI07_11660"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51741.1"
                     /translation="MKNLSLTLCAALLCLPGPSLWGAANEAPDEAAPPRLLWVPESPE
                     TNGDCHVAFRATWELEAERDVEFRLWGASWFVAWLDGEYFAEGPARFPAAFPQYQSFH
                     ARLGAGRHVLAVQVHDEGIPTRMLGEQLPFLYCVAIAGGREIPLRWKQSRIGGYAARA
                     KRINPQLGWIEWCDTQAVPDWQSPGYDDAPWASPITVTRSLGKLEPFPAANPRIFIHP
                     LKPAAAGKLAEMFGYERDNPAARFFLRDLAAASHPAQGVWRRYDLGRVRLMRPRFELD
                     LPPGAVVEFSYSEYLTHGRVAPWITLSAGDSCNLDHYVARGGRQEFFPLTPRGGRFLE
                     VHVLAPPDKVRFLKEEAVERCYYGEPDAAFRCGDKLLNRIWSVGVETHRACAEDALID
                     NPTRERGQWAGDVVTVGMDIAGVAFNDLRLCRRGLVQCAQSARADGMVAGMCPGQDIF
                     LSTYAAQWITACVHYWELTGERALLEELFPAATRNIEAFEAGRTAEGLKDSLAWGFVD
                     WGYVRNPGPSDMGVNLHYLAALRDMQRWCEALGKPERARHYQSLAQAMTAIIARYFAD
                     EFAQGGDAWTRIGYHRAALGLRLGFFKGASETAGLRFLKSHILRCFPNDPRAPRLSDP
                     AADNARLITPYFAHYALPLLIEHGEMNFVLDQYRKCWGWALKDGRTTWLEVFDTRWSH
                     CHQWAGCPTWQLSRYALGLQPRYDLGERHFAVVLAPGSLAQAQGCLPLPGSNETLKIR
                     WKRTAGKVRYRVETPFPIWLHLPARLTGAEPRTVAVDRVFEQSFPKP"
BASE COUNT         1943 a         3932 c         3309 g         1866 t
ORIGIN      
        1 caggccgagc tttctttgtg accaaatgtt acccatttat gcgagtctca ataatgcaaa
       61 tctatcgtgc cccgcgccgg agcaagtggg ttcgtccgga tcgctccgtg cggcagatcg
      121 gggttgtcgc ccggcttggc tcccgcggcg gcaaagagtt tactaacgac catcgcaagc
      181 ggattacgcc aaagcaaaat tatgaagact gttgaccatt gttccgtgcc ctctgtagtt
      241 cagtccaaat cgggggcgaa tcaatccccg tccacagaga tcaatgagga caaaatgaca
      301 ccgacccttc ccccgcgagc gattatcgcc agcttaatct ggctttgcct tgctttcctc
      361 aacagcgcta tggtttccgg gcagaccgcc acaccaggcg cgaaccccga ggagtccggg
      421 attcgctggc tgcttgatcc cgggcgaagc cccgtgcagg tcgcccatca aatacaatgc
      481 acttccggga acagcaacct ctgggagggc atcgtaacca acacgacgga tcccttcctc
      541 gtcttcccgc cagtgcggat tgatgccgac tccgaaaaga ttctgtacct caatatggcc
      601 gtggagacgg gccggcgagt gcagctattc ttttccgcgg atgattcttt tcaggaggaa
      661 ctaagttgcc atgcccgcgg cctcgccgcc ggcagtgagc tgtcgcttta cgccctggat
      721 ctgagcgagt tcccgaagtg gcgtgggaaa atcacgtctt tacgcctgga tctggatggc
      781 agccgcaatg gaacccgcgt aaaatttgcc cgcatcggaa gcggtaaatc cggcataacc
      841 gccggggcgg ttcccctcga agtggaaacc gcagccactc cctgcaccgc caatcgccgc
      901 cgtaacgccg cctttcagga tacgattgtc gccaccgacg aatgttatgg gtcgtccgcg
      961 gtcatgacgt ttgtgaaaac ccacttcagc tacgtcgaac ttaatgtcct cgcgcggctg
     1021 ctggttcgcg ccgaagccga ggggcgacaa tttgaagcgg ctacggccgc gcgcgggttg
     1081 aaagtcgaag atctgccggg cggagttcgc gctcagtacc agctcgcagg cgcgcaaatc
     1141 accagtgaaa tcctcccgct gcggattggc cgtgacaccc ccggccagga cggggccgcg
     1201 ctgtttacca tccgcaccga accgcccgtg ccggtgattg tggattgcgg cgacagcatg
     1261 gtcgaaaacc cctttggcgg cttgtttgcg cacagcgagc cgacgctgct aaatagcacc
     1321 gaccaaatca ccctggaatc cggcaccgcg ttcctggcga gcaaagccca tccgctcaag
     1381 gtcgcgatcc gcggcgatgc cgccctgacg gttaaaacca acctgaacgg cggacagtat
     1441 ctgagcttcc gtgccccgag cggccagttg tctgttttgg tcagctacgc cgaagacgac
     1501 gcccggctcc gcgccattgc ggcaaccgat ttcgcccagg gccgcaagca ggtcgaggac
     1561 tactgcgccg agctgctgca ggcgcgggtg cagacgccgg tcaagaacat tgacgccgcc
     1621 ttccgcaccg cgctctacaa cctcgaatac gcctggaacg ccccctatgg ctggaccgaa
     1681 agcatcaact tttggcatgc gctctggcat atgcagcaca ccgccgggga ggaatggatc
     1741 ggtcatgccg accgctcgcg cctgagcaat gagacccacg cggaaaacct catgccgaat
     1801 ggcgcggtgc cgcaaatgat gcagtatgcc cacaaacgcc gcgactttgg cggctcgaac
     1861 cagttcttcg cctggcaggt gcggcactac tggcacttca ccggcgacct ggaattcgcg
     1921 cgcaaactgg ccccggtgct ggatcgcgtc attgctcaaa ccttccgcga aaatgacccc
     1981 gatggcaact acctcctcgg ctggggacag caaatcggca accaggagga cttcatctcg
     2041 actccgcacg acggcaccag cccggccatc gaaggcatca atatgtttcg cactcgccgc
     2101 gaactcgccc tcgcgctggg ggatcatgaa acggccgcgc gctgtgaagc gcgcatccat
     2161 caaatccagg ccgggctctg ggaaaaactc tggctccgcg acctgggccg gtttgctttc
     2221 taccgcgacc cactgggaga acttcggctg gatggccaat accacagcat gctctatccc
     2281 gtgatctggc agatcgccga ccccctcgac agctattcga tgctgcgcca agtacgcgac
     2341 cgcctgaccg gcaaagacgg tgaagtcttc tgctcggata atttcgtcac ccatgccgtc
     2401 accaccacgg gcgtgcaagc cggcattgcc cagcaaccgt gggccgcctg ggccctggcc
     2461 acagccggcc ggcgcaacga ggtcgtccgg ccgctgcaag ccgccgccaa ctgggtgatg
     2521 gacgacaacc atcgcggagc ctggcccgaa atttcctacg agccaagccc cgcctatttc
     2581 tcgcctccca tcgggctctg ggtggccgcg acggtggaag ccttattcgg actcaaagtg
     2641 gacaagccgg ccggccggct gcaggtcgcc ccctgtttcc cggaccactg gcccgccgcg
     2701 tcattgaatc tgcccgacta ccaggcggaa ttcacccgcc agggagatac gctcgaatat
     2761 gtcgtccgca gccgcgaacc cctcacacgc caggtccgct ggagcctccc gccaggccgg
     2821 gtctcggacg tttcggtgga cgggaagccg gtggaatatc atctcgagcc cggcgtcgaa
     2881 tgcctgtttc tcgcgctgga aaccaagccc agccgcgcat cccgcatccg cgtcaaagtt
     2941 cagccgttca atctggccgt gaatgcaccg gcttccattg ccgagggcga cccgatcacc
     3001 gtgagcgtca acgggatcgc gattgagggc gttgaagacc actgcggcct gctggcggaa
     3061 acaacactcg ccagcagcca acaacttcag ggccgcatca gcaccggcct gctggaggcg
     3121 taccgcggct acggacggct cggccagatg actttcgcgc gccgcacgtt cttcctgcgt
     3181 tgcctgggac cggaccaaac gaaatactgg ctcccggtgg acctggccat cctgccgcgc
     3241 tacgaagccg cgccggcggc caacctccac ctcgacggcg acacgatcag cgcgaagctg
     3301 ctcatccgaa acaacaccgt ccttgccctt aacggcacgg cgtggttgca aatcgcccgc
     3361 gacgaattcc cctgcgcggt cagcatcgcc ccgcgctcgc aggccgaggt gagcgtatcc
     3421 atccccgccc gccaggcggc ttggctgtcc cccggcgata acgcggcctt gctggtcttg
     3481 cccggcaagg ccaccgtcaa ccttgtcttg aacggttcgc cgctcttccg ggaacaggaa
     3541 ccgcttcggc agtatcaaaa agcccggata aaagccatcg cgctgccgcg cgaaaatctg
     3601 atcccggaca cccagtggaa tacactgcgc tcatttaagg cttatgtgca tccgccgtgg
     3661 tcgggtttgc agccgccgct gctcgccctc ggcaaccaga ccgaagtcac cgccggcgga
     3721 ctggaggaag tacgcttcca gctcgcgccg cggcaattcg tccccatctc ccatgcgagc
     3781 ggccggccca gttggagcct ttcgctaagc aacctgccgt gcaaaaaact ctatctgctc
     3841 gtcattcctt tcacggacaa ccacgacgtg tattcacccg tggcccaggt ggcggtggaa
     3901 accgcggagg gcgccgtgct ggcccgcacc ctgcgcgtgc cgggcgacct ggattggtgg
     3961 gcgcccccgg ccattgtggg cgagttcgcc tcggcgcggc agcctcggaa agaccgcttc
     4021 ggcctgctgc cgctgctgaa tccggcgcaa ggcgactggg aggaaggcca gccgcccgca
     4081 tttccgcaat ccgcttattg ggccacgtgc cgccagctcc acctgagcag cgccgtcctc
     4141 aatgttgtcg aaatggacct gggccgtcca cggctgctga agtcgctgac cgtctccacg
     4201 ctgggatccg accccgcttt gggagtggtg gccctggccg cagaaaccga ccagggcctc
     4261 gaagcgctcg acggcacccc ttacgccccg ccagcgaagt tcgccgcagg ccgggtattg
     4321 tttgccttcc agcagccgga tgacacgctg ggctggcaac tcgagggcga cgccttcagc
     4381 gtgtccgccg acccgcaact gtttccatcg ttcccgaccc tcaactccgt cgcgcgcgcc
     4441 ggcgaaaaag cgactggccg ggccacttcc cccgagttca ccatccggcc ccatgaacac
     4501 tggatgctcc tggaaatgca cggcggccgc tcggccagcc ccggcggcga ctgcaacctc
     4561 tgcgttcgcc tggtggacgc gcagtccggc gcggtcctcc gcgaagtcct gcccaccgga
     4621 tcccatctca ttgccgaaga gcgcgtcggc ctggaaggat tgcaaggcaa gcgcgtgcgc
     4681 ctggagctcg tggaccgcaa ctcggccccc acctatgcct ggattggctt gcgccgggtg
     4741 acccttaccg gaaacgaact atgaatcgcc cgaaatcccg aaacggaatt gtccgccggc
     4801 cttcgcccgg agttcaaacg cgccgcttca tcgccgccgc cctcctcgcg ctcggcctgg
     4861 cttgctggcc ccggccgtgc cccgcccaac ccgccgccac caacggaatg gcggcgactc
     4921 tggcgtttgc ggccggcacg ctcgaggggt gggtcgtcga gggggagaat acctggcgca
     4981 tcgggaccaa cccgccgctg tttacgagcc cggctgaccc gatccggttc gtggtaaact
     5041 cgtatgaagg cggggaacgc aaccgcggcg tgctgcgctc gccccccttt gtcattgagc
     5101 acgaacggca acgattctcg atcgccggct gggacggcac caccaacggc gtcaatgacg
     5161 ccatgctgaa cttcgtcctg ctccgctccc accccgacgg cgaaatcctg cggcgcaccc
     5221 acacgcccgg cggcaacaaa ctcacccccg cctcctggca aacgacggac ctgatcggcc
     5281 ggcgcgtatt tctcgaggtc gtggacgcca acccggttct ccgcccggac ggcggttacg
     5341 catggatcgc cttcgccgat taccggcagg agtccgcggg cctgttcacg aagccggtgc
     5401 ggcgggatga tttatacggc ctgcgcattg acgcaaactc ggagcgaatc ctgtgccgca
     5461 ccgtgccttt cttcgccgcg ccgccaacgc agcgcatgcg gaccacgcgc tcggttcagg
     5521 gccaccgcga gaccattccc gtcggcgccc gggccgaagc gctgtgcctg ctcggcatga
     5581 tcaatcatgg gtgggaggcc ggcaccgcgc attggggcga acacccggaa ttgcggccga
     5641 agcgcgacga ccaactctac gtcggcgcaa acatcgggga gatcgaactt tgttatgccg
     5701 gcggcgcccg ggaccgcgtg ccgctggtga tcggcgccac ggcgtggttt gcggggttct
     5761 ggggctatag ccagttcagc gacgtgcggg ttctgccccg ggagcctttt gcctcgcggc
     5821 cggaatacat ggccgtgctt caacgcgccc tgcgcctgcg ccagagcgag gagccgggca
     5881 gcgccgaaaa cgcccacgcg ctctacttcc tcaccgtaaa accacgcccc caaaagattg
     5941 aatccatcgt ggtgcacgac aacccggcca accgcgggca gccgctcgtc acagccatca
     6001 ccctggtcgg ggccaaaccc gccaccaacc tgctgcactt cggcacatgc cgcctggagg
     6061 cggcggacct ggcccccgcc tttagcaccg cgcgcccggg cgactggtcc cgggacctcg
     6121 ccgccctggc ccgggtgctg tacacgcgcg acgcggacct gccccggaag gtggcgcccg
     6181 tggaagtgcc cgccgatttc aaggcggcgc gcattcgctt tcgcggcggg ccgctggcgg
     6241 acatgctctc caacatctgg ctcgcgaacc tctaccaaat ctcccagaag ttcgagcgcg
     6301 gctcgggctt cttccacgaa acggagacca acagcccctg gtatggcagc tacagcggca
     6361 tcggcacctg ggcgcacctg ggcgtctatg ccccttacgc ctactcgcgc tgctccgatc
     6421 atttcgcgtc cctggccctg cggtgcattg acgatcccgt ccgggtggcc agctatatgg
     6481 atttcgtgga ctactggttc tactggcatc gcgataacca cgatcccgac caaggcccgc
     6541 ccaacgacgt gctcgacgtg gcccgctacc ccaaaggggt gaccgggcac tggagtttcg
     6601 gcctcccccc gggcctgccc ccctggcagc tcaacgagat ccccggcgtc gaggaaatgg
     6661 acggccacgg cgccaccatg gtggcgcgct ggctggcctg gcgcgtgctg ggcgcccgca
     6721 ccgaccaatg gctgaccggc gcgcgggaca acgtcttcgg caagagccgc tgggacacca
     6781 cccgcgatgc cgccgagttt gtctgctggc tcatggacca caccgggatg gatgtcatct
     6841 actgcgaggg ggaaaccacc ggctgggcgg gcggccccca ggtgccgctc accggcgcgc
     6901 cggattggtg gcaggaaacc gatcccgtga agctgcggaa gaactacgcc aacgccaacg
     6961 cctacgaagt ctatcccacg tacgtctgcc tgaccgcgct gcgctgttcc gcgcaaatcg
     7021 ccgacgcgct cccggacgcc gctctcgccg ccaagtggcg ccgctatgcc gaccggctgg
     7081 agtccggcat gctccgcctc ctggccgtgg gcgaattcgg cagcctgacc tggagactca
     7141 gccgccactc cgtctatccg agcggccagg attccctcgt ccaggcctgg tttgcgttct
     7201 atttcgacgg gctcgacccc aaccgcctcc cgccgcaggc gaccggcatc acgcgcaaca
     7261 cgctgcgccg ccagctcagc ctgccgtgcg gccgcgcccc ggcgctggcg atgggctacg
     7321 gcctcggctg gctttgcaaa gccgcgctgg tgctcgacga aatggacgaa gccggcccga
     7381 tgctgctgaa tatcgcgcgc tatacctacg acaaaaacat ggactacgcc gatccctcgc
     7441 ggggcattga ctggcgccgc ttcgcgtgga tcgtgccgga aggcgccaac atccttcccg
     7501 acggaagctg gcatcgcatc ggcgacctca gcaacggcgc aaaccagggc ccgccgctgc
     7561 acgccatgga aatctgcgcc ggcttcgacg acaccaaacc gaatgacttg cggctgctgc
     7621 cccgcgtgcc gaatccgctc acgggcatcg aggtcgagga tttcatggtg ctcgtcccgg
     7681 acggcgccgg cctggcccgc gccaaagtca gctacgccta taatcgcgcc gccgcctcat
     7741 tccgcctccg ctcggaccgc cccctgcccc ggctcgccct ccggctggga ccgttccacg
     7801 cagcggaagc gcgccgcgcc gcccaaaccc tcaaatcatc cgcgaaaacc gatgtccgca
     7861 cggaaagttc gggaaccttt caggcgcagg ctgcctggtg ggtgtggctg gagaacctga
     7921 aagacattgc ctccctcgac cttgattgga aatcctgagt aatgaaaaac ctgtccctca
     7981 cgctctgcgc ggcgctgctg tgcctgccgg gcccgtccct ttggggagcg gcaaacgagg
     8041 cgcccgatga agccgcgccc ccgcggctgc tctgggtccc cgagtccccg gaaacgaacg
     8101 gagactgcca tgtggccttt cgcgccacct gggagctgga ggcggaacgc gacgtggagt
     8161 ttcgcctttg gggagccagt tggttcgttg cctggctgga tggagaatac ttcgccgagg
     8221 gaccagcccg tttcccggcc gcgtttcccc aataccaatc cttccacgcc cggctggggg
     8281 cggggcggca cgtcctggct gtgcaggtgc atgacgaggg catccccacc cggatgctcg
     8341 gagagcagct cccgtttctc tattgcgtag cgattgccgg cggccgggaa atcccgctgc
     8401 gctggaagca atcgcgcatc ggcggctacg ccgcgcgggc caaacgcatc aaccctcagc
     8461 ttggctggat cgaatggtgc gacacccagg ccgtgccgga ttggcagtcc cccggatacg
     8521 acgacgcccc ctgggcctcc cccatcaccg tcacccgctc actggggaag ctggaaccct
     8581 tccccgccgc caacccgcgc attttcattc atccgcttaa accagccgcc gccggcaaac
     8641 tcgcggaaat gttcggctat gagcgggaca acccggccgc ccgcttcttt ctgcgcgacc
     8701 tggcggcggc cagccatccg gcccagggag tctggcggcg ctacgacctg gggcgcgtgc
     8761 gcctgatgcg gccccgcttc gaacttgatt tgccgccggg ggccgtcgtc gaattcagtt
     8821 atagcgaata cctcacgcat ggccgcgtcg ccccgtggat cacactttcc gccggcgact
     8881 cctgcaacct cgaccactac gtggcgcgcg gcggccgcca ggaattcttc cccctcacgc
     8941 cccgcggcgg ccgcttcctc gaagtccacg tcctggcgcc gccggacaaa gtgcgcttcc
     9001 tcaaggagga agccgtggag cgctgctact acggcgaacc ggacgcggcc ttccgctgcg
     9061 gcgacaagct gctcaaccgc atctggtccg tgggcgtcga aacgcaccgg gcctgcgcgg
     9121 aggacgcgct cattgacaac cccacgcgcg aacgcggcca atgggcgggc gacgtggtca
     9181 cggtgggcat ggacatcgcc ggcgtggcct tcaacgacct gcggctctgc cggcggggcc
     9241 tggtgcaatg cgcgcaaagc gcgcgcgcgg acgggatggt ggcgggcatg tgcccggggc
     9301 aggatatctt cctgtccacc tatgccgccc aatggatcac cgcctgcgtg cattactggg
     9361 agttgacggg cgagcgcgcc ctgctggagg aactgttccc ggccgccacc cgcaatatcg
     9421 aggcgttcga agccggtcgc acggctgagg gcttgaaaga ctcgctcgcc tggggatttg
     9481 tggactgggg ctatgtgcgc aatcccggcc ccagcgacat gggcgtcaac ctgcactacc
     9541 tggccgcgct ccgcgatatg cagcgctggt gcgaggcgct cggcaagccc gaacgcgccc
     9601 gccactatca atcgcttgcc caggcgatga ccgccatcat cgcccgctac ttcgcggatg
     9661 agttcgcgca aggcggcgac gcctggacgc gcatcggcta ccaccgcgcc gccctgggcc
     9721 tccgcctcgg ctttttcaaa ggcgcgagcg aaaccgccgg cctgcgcttc ctcaaatcgc
     9781 acatcctgcg ctgtttcccc aacgacccca gggcgccgcg cctgagcgac ccggcggcgg
     9841 ataatgcgcg cctcatcacg ccttactttg cgcactatgc cctcccgctc ctcattgaac
     9901 acggagaaat gaacttcgtg ctcgaccagt atcgcaagtg ctggggctgg gcgttgaaag
     9961 atggccgcac cacatggctc gaagtcttcg acacccgctg gagtcactgc caccagtggg
    10021 ccggttgccc gacctggcaa ttatcccgct acgcgctggg cctgcaaccg cgctacgacc
    10081 tgggcgaacg ccatttcgcg gtcgtcctcg cccccggctc cctggctcaa gcccagggct
    10141 gcctgccctt gcccggcagc aacgagaccc tgaagatccg gtggaagcgc actgccggca
    10201 aagtgcgtta ccgcgtcgaa acgcccttcc ccatctggct ccacctgccc gcccggctca
    10261 ccggcgccga accgcgcacc gtcgccgtgg accgcgtgtt cgagcagtcc ttcccgaaac
    10321 cataaaccac gccgcgcttc gcccacgtcc gcaggcgggc ttttgcccgg cccaaacgcc
    10381 ggccctttcc ctgcccgtcc gcgcccggaa gcgacggcca gcaactccag ttcaaagaca
    10441 accacctccg ctcaaccggg cgaaccccct gcccgcgatg aggacaacgt aaatagtcgc
    10501 cttgcggcaa cctccgtccg ggaggggaaa tgtccccgcc aaccgtgacc ccatcccgcc
    10561 caaaaaccag ggcacgcgac aacacccggc cgcccgttcc gggcatgccg aacgttcgcg
    10621 tccactccct cccgccttga aacgtcgttt tacatcatcc gcgagtcgtc catccctccc
    10681 ttccggctca ggcccgcgcc caaacccgac ccacccgccc cagccagccc aacgctccca
    10741 ttaggctgtg tacaacgggg gtacaaagga ggctcaaagg gggctcatgc ttattttcta
    10801 tgtgctcctg ccgggcttcc gatgtaatcc ccttgtacat ggctggaaag gacggggaag
    10861 gaacggcctc gcgggcggcg gtggatgccg gggcggcggc gggcttcgat ttcttaaccc
    10921 ttgccgtttc agccgcttgc tgagatttca acatcggcgg cggcgggacg atggcgttgt
    10981 gaacccggct ttggcctttc cagccgttcc gggtctgaac cacggcgata aataccttct
    11041 gccccggggc
//
LOCUS       DAOPQG010000117        10824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_6946_length_10824_cov_17.304021, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000117 DAOPQG010000000
VERSION     DAOPQG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6946_length_10824_cov_17.304021"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1228)
                     /gene="istA"
                     /locus_tag="PKI07_11665"
     CDS             complement(32..1228)
                     /gene="istA"
                     /locus_tag="PKI07_11665"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOC51742.1"
                     /translation="MNQLKVKQQQTIVALREQGWSICRLARELGLDRKTVRRYLRQAA
                     DSKSPTNPLTGSPPKSPTPQTGSLALGGAGPDSLCEPWKEVIEAALTSGLSIQRIYQD
                     LQQEHQFGGSYFSVRRFARRLSQKLELPFRRIEVEPGAELQVAFGQGAWIIDESGKRR
                     CSHLFRAVLSHSRKGYSEAVWRQTTETFIRCLEKAFRYFGGVTQSVIMDNLRAAVNHA
                     DWFDPELNPKLEDFCRHYGCVILLCKPKMPRHKGKVESGVNYGQENALKGRSFESLGR
                     ENLFLLEWESNVADKRIHGTTKRQVQKVFNEGERPALRPLPASLFPFFEEAQRAMHRD
                     GHVELKGADYSGPPEYVARKVWARWEHRLVRIYNRRMEQIALHARAEPGKFSTDPAHI
                     HPNCRS"
     gene            complement(1540..2328)
                     /locus_tag="PKI07_11670"
     CDS             complement(1540..2328)
                     /locus_tag="PKI07_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC51743.1"
                     /translation="MADLSIIITSHNKPRFVKEAVQSLLDQTHCHWQGILVDSGVLLK
                     QGFFNYLSDPRLKVVPSGETPELSRTKIMASWCFNRILNSGELTGELIMYLNDDDLLY
                     PEAFATFWGYYQQHAREPQAMYASQDIGLVAPDGQTRIIGRRIADRPGGRFCKGRRMD
                     CRVDYLQFCHTAKILGRFREVYKTSQYHSEDKRDAHHADGIFMEQIGALTKIHNLAKV
                     LSMNRRTTESANLEYFASAFGRVMVTIRAKLRGARERFFSSRRY"
     gene            complement(2335..3894)
                     /locus_tag="PKI07_11675"
     CDS             complement(2335..3894)
                     /locus_tag="PKI07_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC51744.1"
                     /translation="MNRQLDVLFVSPNSSFQAYQALSKDFAAIEPPTWALLLAQSCRA
                     KGFGVGILDCCAERLTVEESVKRIVEADPRLVCMVVYGQNPNSGTTNMIGATALARAL
                     KQQHPDYRICFVGSHTSALPKEVLALNCVDLVLLNEGVYALHNLLQTNLKTDLSKVRG
                     IGYKQDGVPVLNPPERVVPQDRMDADLPGSAWDLLPYRQRPLDLYRAHFWHAEFNHDF
                     RTPFAAIYTSLGCKFRCDFCMINIVNRIDNADGVAASHSPNMRFWSPGFIISEFEKLA
                     QLGVETLRISDEMFFLNKRYFEPLLNLIIERGLKLRMWSYSRIDTVQPHYLDLFRRAG
                     INWLALGVEAGNQTIRHEVSKGSFKDTNIRQVAQTIRNHGLNIIANYIFGFPEDNLET
                     MQQTLDLALELNTEMANMYPCQALPGSSLYYLARANGWPLPDSPAGYGFLSYESQPMP
                     TRHCSATEVLRFRDQAWQKYFTHPDYLALVERKFGFEQRRNVQAMASIPLRRKLLGDP
                     PPEIDALVTKG"
     gene            complement(3912..5066)
                     /locus_tag="PKI07_11680"
     CDS             complement(3912..5066)
                     /locus_tag="PKI07_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOC51745.1"
                     /translation="MHNNITRADLDALIAYLGQDNPMLTQSRQVRQFEQEWSNWLGVR
                     HSVFVNSGSSANLLTMAALRERLGPGEIIVPALTWVSDIASVIQCGFQPVFVDINPRT
                     LGMDDQEVIAHLRPQTRAVFITHILGYNALTQRLLEVLQARNIPLIEDVCESHGATFQ
                     GRKLGSFGLMSNFSFYYAHHMSTIEGGMISTNDADFYEALRMFRSHGMVREASSETLK
                     ESYRQQYPDLNPDFIFAFPAYNMRGTEINAVLGRAQLRRLDQNNERRRANLRLFLDHL
                     DPQKYRTDFELEGSCNYAFTLILRQPDPTLSAKVEQRLREARVEFRRGTSGGGNQLRQ
                     PYARKLLGDREFEKFPKVDHVHFFGYYIGNYPELERDKILALCALLNQIE"
     gene            complement(5095..5631)
                     /locus_tag="PKI07_11685"
     CDS             complement(5095..5631)
                     /locus_tag="PKI07_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019112131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51746.1"
                     /translation="MKYFDELCRAMEYLARDPRVLFLGQAVACPGTAMSNTLKNVARD
                     KLLELPVAEEMQMGMSSGLALVGHVPVSIFPRWNFLLLAVNQVVNHLDKFSLMSNAGY
                     QPKVIIRTGIGSVRPLHPQHQHVGDFTEAFRLMCPNIEVIRLEEPKEIFPAYQKALER
                     EDGKSTIVVEYGDYYNEK"
     gene            complement(5635..6234)
                     /locus_tag="PKI07_11690"
     CDS             complement(5635..6234)
                     /locus_tag="PKI07_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC51747.1"
                     /translation="MTANDLINFETEIANLFNAAQIRAPIHLYYGNEAQMIDLFRDVR
                     PEDWVCCSWRSHYQCLLKGVPPEKVREEILAGHSISLCFPEYRVVSSAIVGGILPIAV
                     GIAMGIKRRGETARVHCFMGDMTAETGIAHECLKYSHNHQLPIRFIVEDNGKSVCTDT
                     REAWRQPALTCEQTPHPMVVHYQYQTKYPHAGAGVRVQF"
     gene            complement(6237..7319)
                     /locus_tag="PKI07_11695"
     CDS             complement(6237..7319)
                     /locus_tag="PKI07_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HOC51748.1"
                     /translation="MVISRSPYRISFFGGGTDYPAWFRENGGAVLGTAIDKYCFITCR
                     YLPPFFEHRLRLVYSKIEDCQCAADIQHPVVRTVLEMLRVERGLEIHHDGDLPARSGM
                     GSSSAFTVGFLHAVYGLLGRLTSQNQLATDAIHVEQDLIGETVGSQDQIFAAYGGFNR
                     IEFKRDNSFRVEPVPLSRKRFEELNDSLMLFYTGLRRTASEVAASYVVDIGARTRQLT
                     RMRQMVDEGLQILADGGSLDRFGDLLDEAWQLKRSLSGAVSNSQVDEIYAEARRAGAR
                     GGKLLGAGGGGFLLFYVPPGCQQQVKERLRNLLWVPFRFDTSGSQIIFFSPGQDYAEL
                     DQARSAQAGPAFREVFPPEGASGKQT"
     gene            complement(7732..8910)
                     /gene="hemW"
                     /locus_tag="PKI07_11700"
     CDS             complement(7732..8910)
                     /gene="hemW"
                     /locus_tag="PKI07_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOC51749.1"
                     /translation="MTRLDAEPVAGLYVHVPFCVQKCAYCAFYSEPAKGELIERYVGA
                     LILELERIAHDLRPRTIYFGGGTPSLLDSRQWQRILRAWDRLGLGKTTEWTVECNPAT
                     ICLEKAKLLRDAGVNRFSLGVQSFNEALLQRLGRVHTRREIFQSFDLLRKAGFDNLNL
                     DLMFAIPGQTLEAWRETLHEAFALGSEHLSCYEVIYEEDTLLYQQLETCPTAADEDLA
                     CAMYEELLEQATNASLKQYEIANFACDRALSRLPQTANLPSHACQHNVNYWRGGDFYG
                     LGPSATSYVRGVRTQNSPDTRRYCEQLEQGQRALEYREELPPLARAGEAAAFGLRMPS
                     GWPFDQFRQITGYDLRQQWSEEMNLLVQRGWGRILSDRFYLTPQGLRFADDAALLFLR
                     "
     gene            complement(8971..9345)
                     /locus_tag="PKI07_11705"
     CDS             complement(8971..9345)
                     /locus_tag="PKI07_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51750.1"
                     /translation="MTQPLALVFYERLLPGSQLVNRLQDLNYRVQVIVNADQLVECAE
                     QAKPMLVLADLKSKQNNVCPALARLKQNPTTRHLPVVAFCGENDAEWQEATQMGGVTL
                     LVSEAAILNHLPQFLEQALQVE"
     gene            complement(9502..10164)
                     /locus_tag="PKI07_11710"
     CDS             complement(9502..10164)
                     /locus_tag="PKI07_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51751.1"
                     /translation="MVDGCPVRYRGAGWGKKKTQQLRVEGHELKLGLFYRHAAAARTE
                     AGRGILSDKVGVSWQDPAMGLGQRRHWEAPREGLGRARNVRFLGDGAEWVWNLKKDRW
                     PKALELLDFYHGSQHLWSLGRALRGETQPALSRGVKPLRHKLRRGQEQKALRQMARFR
                     HPRGEVGEIIRREQNSFQSHAERMRYQRMARRGWPIGSGAVESACRQKQTRFKRCGQF
                     WT"
BASE COUNT         2338 a         3110 c         3123 g         2253 t
ORIGIN      
        1 ttatgcacgc aactccgtat ctaacctgcc ttcaactgcg gcaattagga tgaatgtggg
       61 ccggatcagt ggaaaacttt cccggctcgg cccgcgcgtg caaggcgatc tgctccatgc
      121 gccggttgta aatccgcacc aggcgatgct cccagcgcgc ccagactttg cgagcgacgt
      181 attcgggcgg ccccgagtaa tcggctcctt tcaactcgac gtgcccgtcg cgatgcatgg
      241 cgcgctgggc ttcttcaaaa aacgggaaca ggctcgccgg cagcggccgc agcgcgggcc
      301 gttccccttc gttgaacact ttttggacct gccgcttggt ggtgccgtgg atgcgcttgt
      361 cggccacgtt gctttcccat tccagcagaa acagattttc ccggcccagg ctctcaaagc
      421 tgcgaccctt gagcgcgttt tcctgaccgt agttgacgcc cgattccact tttcccttgt
      481 ggcgcggcat tttgggtttg cagagcagga tcacgcaccc gtaatgccgg caaaaatctt
      541 cgagcttggg attgagttcg gggtcaaacc aatcggcgtg gtttaccgcc gcccgtaagt
      601 tgtccatgat cacactctgg gtgacgccgc caaaataccg gaaggccttt tccagacagc
      661 gaatgaacgt ctcggtggtc tgccgccaca cggcctcgct gtagcccttg cgcgaatgcg
      721 agagcaccgc ccggaacaga tgcgagcacc gccgcttgcc actttcatca atgatccagg
      781 cgccctggcc aaaggccacc tgcaactccg cgcccggctc cacttcaatc cggcgaaagg
      841 gcagttccaa cttctggctc aagcgccgcg cgaaacgccg caccgaaaag tagctaccgc
      901 caaactggtg ttcctgctgg agatcctgat aaatccgttg aatggaaagt ccggaagtca
      961 gcgccgcctc aatgacctcc ttccacggct cgcacagact atccggaccg gctccaccca
     1021 aggccaaaga gccggtttgc ggggtgggcg attttggtgg tgagccggtt agcgggttgg
     1081 tgggggattt tgaatcagcg gcttggcgca gataccggcg caccgtcttg cgatccaagc
     1141 ccaattcacg cgcaaggcga cagatggacc agccttgctc acgcagggca actatcgttt
     1201 gctgttgttt cactttgagt tgattcatcg ccccattctg tccgcatccc gcgaacagtg
     1261 ggaacgatgt ctcgcctcaa agggtgggtg actttaaccc gcaaattggt gggtgacttt
     1321 gcccgcaaac tgacagccac ccagggcgcc ggtagtccag ccaaatcacc ggcaaccgga
     1381 aaatgcgccc tggccagttt cacttttgtt tgtctcaccc gccagttagc gagtgttact
     1441 tgcgcacagt ttctcaccgc gtcgataaaa atacctgccc gcgctgaaag ggtcgtggta
     1501 tttccattac tacccgcctt ggcgaagcag caagttccgt tagtagcgcc gggaggagaa
     1561 gaaccgctcg cgagcgccgc gaagtttggc gcggatagta accataacgc gcccgaaggc
     1621 ggaagcgaag tattccaagt tagcgctttc agtggtgcga cggttcatgc tcagaacctt
     1681 ggccaagtta tggattttag tcagcgcacc gatctgctcc atgaagatgc cgtcggcatg
     1741 gtgggcgtcg cgtttgtctt cggagtgata ctgggaggtc ttatagacct cgcggaatcg
     1801 gccgaggatc ttggccgtat ggcagaattg gaggtaatcc acacggcaat ccatccgacg
     1861 gcctttgcag aaacgaccgc cagggcggtc ggcgatacga cggccgatga tacgagtttg
     1921 gccgtcggga gcgaccaagc cgatgtcctg cgaggcatac atggcctgcg gttcgcgagc
     1981 atgctgctgg taatagcccc aaaaagttgc aaaggcttcc ggatagagca ggtcgtcgtc
     2041 attcaggtac atgatcaact cgccagtgag ttcgccggag ttaagaatgc gattgaaaca
     2101 ccaggaagcc atgattttag tgcgggatag ctctggagtt tcaccggaag gaacgacttt
     2161 caggcgcggg tcgctaaggt agttaaaaaa accttgcttc aggagtacgc cgctatccac
     2221 caaaatgcct tgccagtggc agtgggtttg gtcgagcagg ctttgaacag cttctttgac
     2281 gaagcgcggt ttattgtgcg atgtaatgat gatgctgaga tcagccattc cctatcaccc
     2341 tttcgttaca agggcgtcga tctcgggcgg aggatcaccc agaagtttgc ggcgaagcgg
     2401 gatactagcc atagcctgaa cattacgccg ttgttcaaag ccgaacttgc gttcgacgag
     2461 cgcaaggtaa tcggggtgag taaagtattt ctgccaagcc tgatcgcgaa agcgcagaac
     2521 ttcagtggcg gaacaatgcc gggtgggcat gggctggctt tcgtaagaga ggaaaccgta
     2581 gccggccggc gagtccggta atggccagcc attcgcgcgg gctaggtaat agagcgaact
     2641 gccgggcaag gcctggcagg gatacatatt ggccatctca gtgttgagtt cgagggccag
     2701 atccaacgtc tgctgcattg tctccaagtt atcctcggga aacccaaaga tatagttggc
     2761 aattatattc agaccgtggt tacgaatggt ttgggctact tggcggatgt tggtgtcctt
     2821 gaacgagcct ttggaaacct cgtgtcggat agtttgattg ccagcttcga cgcccaacgc
     2881 gagccagttg atgccggcgc gtcgaaacag gtccagataa tgcggttgaa ccgtgtcaat
     2941 gcgcgagtag gaccacatcc gaagcttgag cccgcgttcg atgatgaggt taagcagagg
     3001 ttcaaaatag cgtttattga ggaagaacat ctcgtccgag atgcgcagag tttcgacccc
     3061 caattgggct aacttctcga attcgctgat gatgaagccc ggcgaccaga agcgcatgtt
     3121 gggagaatgg gaggcggcta caccgtcggc attgtcaatg cggttgacga tattgatcat
     3181 gcaaaagtcg cagcggaact tgcatcccag cgaggtgtaa atagcggcaa agggagttcg
     3241 aaagtcgtga ttgaattcgg cgtgccagaa atgggcgcgg tagagatcca gcggtcgttg
     3301 ccgataaggc agcaagtccc aagcggagcc cggcaggtcg gcatccatgc ggtcctgcgg
     3361 aaccacgcgc tcgggcggat taaggacggg cacgccatcc tgcttgtagc cgatacctct
     3421 gactttggac aggtcggtct tgaggttggt ttgcaacaga ttgtgcagag catagacgcc
     3481 ctcgttgagc agaaccaggt ccacgcaatt caacgcgagc acttctttgg gcagggcact
     3541 ggtgtgcgag ccgacaaagc agatacgata gtcggggtgc tgttgtttga gtgctcgcgc
     3601 cagcgcagtg gcgccgatca tattagtcgt gccggaattg gggttttgcc catagaccac
     3661 catgcacacc aaccggggat cggcttcgac aatacgcttg accgattcct cgaccgtcag
     3721 tcgctccgcg caacagtcca gaatgccgac cccgaagcct ttggcgcgac aactttgggc
     3781 cagcagcagt gcccaggtgg ggggctcgat agccgcgaag tccttgctca aagcctggta
     3841 ggcttggaag gatgaattag ggctgacaaa aagtacatca agctgtcggt tcatgggtag
     3901 ggcgcgttgg gctactcgat ttggttgagc aaggcgcaca gggccagaat cttgtctcgc
     3961 tccaattccg ggtagttgcc gatataatag ccgaagaagt gcacatggtc tactttggga
     4021 aacttctcaa attcccgatc gccgagcagt ttgcgggcgt agggctgccg aagctggttg
     4081 ccgccgcccg aagtgccgcg ccggaactcg accctggcct cgcggagacg ttgctcgact
     4141 ttggcactta aggttgggtc gggctggcgc agaatgagcg tgaaggcgta gttgcagctt
     4201 ccctccaatt caaagtcggt gcgatacttt tgcgggtcga ggtggtccag aaacaggcgc
     4261 aggttcgcgc ggcggcgttc gttgttctgg tccaaccgcc gaagttgggc gcggccaagc
     4321 acggcgttga tctccgtgcc gcgcatgttg taggccggaa aggcgaagat aaaatcaggg
     4381 ttcaggtcgg gatattgctg acggtagctc tctttgagag tttccgagct tgcctcgcgc
     4441 accatgccgt gcgagcgaaa catgcgcagg gcctcataga agtcggcgtc attggtcgaa
     4501 atcatgccgc cctcgatggt gctcatgtga tgcgcataat aaaatgaaaa gttagacatg
     4561 aggccaaagc tgcccagctt gcgcccctgg aaggttgcgc cgtgggattc gcacacatcc
     4621 tcgatgagcg gaatattgcg ggcctgtagt acctccagca atcgctgagt cagagcgtta
     4681 tagccaagga tgtgggtgat aaaaaccgcc cgggtctgcg gacggaggtg ggcaatgact
     4741 tcctggtcgt ccatgcccag cgtgcgcggg ttgatgtcca cgaacacggg ctggaagccg
     4801 cattggatca ccgaggcaat atccgatacc caagtgaggg cgggcacgat gatttcaccc
     4861 gggccaagcc gttcacgcag cgcggccatc gtcaacaggt tcgcggagga acctgaattg
     4921 acaaagacgc tgtgccgaac cccgagccaa ttggaccact cctgctcgaa ctggcggacc
     4981 tgccgggact gggtcagcat cggattatcc tggccgaggt aagcaatcag cgcgtctaaa
     5041 tcggcgcgcg tgatgttatt atgcatcagc ggccaattca aaggcgccgc aggattactt
     5101 ctcgttgtaa tagtcgccat actcaaccac aatggtgctc ttgccgtctt cccgttccag
     5161 ggctttctgg taagcgggaa aaatttcttt tggttcctcc aaacgtatca cctcgatatt
     5221 cgggcacatc agacggaagg cttctgtaaa atcaccaacg tgctgatgct gcggatgcaa
     5281 tgggcgcacc gagccaatgc cggtgcgaat gatcactttg ggctggtagc cagcgttgga
     5341 catgagggaa aatttgtcaa gatgattgac gacttggttg acggccagta atagaaagtt
     5401 ccaccgtggg aaaatgctta ccggcacgtg gccgaccaga gccaggccgc ttgacatgcc
     5461 catttgcatt tcctcggcca ccggcaattc gagcagcttg tcccgcgcga cattcttaag
     5521 ggtgttggac atggcggtgc cagggcaggc cacggcctgg ccaagaaaaa gcactcgcgg
     5581 atcgcgcgcc agatactcca tcgcccgaca caattcatca aagtatttca tgggctaaaa
     5641 ctggacccgc acgcccgcgc cggcgtgggg atatttggtc tggtattgat aatgaaccac
     5701 catcgggtgg ggcgtttgtt cgcatgtcaa cgcaggctgg cgccaagctt cgcgcgtatc
     5761 ggtgcagact gactttccat tgtcttcgac gataaatcgg atcggcaact ggtgattatg
     5821 ggaatacttg aggcactcgt gggcaatgcc ggtttccgcg gtcatatcgc ccataaaaca
     5881 atgcacgcgc gcagtctcgc ctcggcgctt gattcccatt gcaatcccca cagcgatggg
     5941 caggatgccg ccgacgatgg ccgaagacac cactcggtat tcgggaaaac acagagaaat
     6001 ggagtgcccg gccagaatct cttcgcgcac tttttcaggc ggaacgccct tcagcaaaca
     6061 ctggtaatgg ctgcgccacg agcagcaaac ccaatcctcc gggcgtacgt cccggaagag
     6121 gtcaatcatc tgcgcctcgt tgccgtagta aagatggatg ggagctcgaa tctgcgccgc
     6181 gttaaagagg ttggcgattt cggtctcgaa gttgatcagg tcgtttgccg tcatatttaa
     6241 gtttgtttgc cggacgcacc ctccggggga aagacctcgc ggaaagccgg tcccgcctgt
     6301 gccgagcgag cctggtccag ttcggcgtaa tcctgcccgg gagaaaagaa gatgatttgg
     6361 ctgccgctgg tatcgaaccg gaaaggcacc cacagcaagt tgcgcaggcg ctctttcact
     6421 tgttgctgac acccgggcgg cacataaaac agcaggaagc cgcctccgcc cgcccccagc
     6481 aacttccctc ctcgtgcccc ggctcgccgg gcttcagcat aaatctcgtc cacctgtgag
     6541 ttggagaccg cgccgctgag gctgcgcttt aattgccaag cctcatccag caaatcccca
     6601 aagcggtcca acgagccgcc gtcggcaagt atctgcagcc cctcatccac catctgccgc
     6661 atgcgcgtta attgccgggt gcgcgcaccg atgtccacca cgtagctggc ggccacttcg
     6721 gaagcagtgc gccgcaaacc ggtataaaag agcatcagcg agtcattcaa ttcctcaaac
     6781 cgtttgcgtg acagcggcac cggttcgacg cgaaagctgt tgtcgcgctt aaactcgatc
     6841 cggttgaacc cgccgtaggc ggcaaaaatc tggtcctgtg acccaacggt ctcaccgatc
     6901 aggtcctgct cgacgtggat ggcgtccgtg gccagttgat tttgagacgt caggcgcccc
     6961 agcaagccgt acaccgcatg gaggaaccct acggtaaagg ccgaactgga acccatgcca
     7021 ctgcgcgccg gcaaatcgcc gtcgtggtgg atttccagtc cgcgttcgac gcgcagcatc
     7081 tccaacacgg tgcggacaac cgggtgctga atgtcagccg cacattggca atcctctatc
     7141 ttggaataaa ccaagcgcag acggtgttca aaaaaaggcg gtaagtaccg gcaggtgata
     7201 aaacagtatt tgtcaatcgc cgtgcccagg acagcaccgc cattttcccg gaaccacgcc
     7261 ggataatccg tgccgccacc aaagaaggaa atgcggtaag gcgagcggct gattaccata
     7321 atatgcccgt gcctgcgcct tgcctgccgg gaatccgact cgaaccgcat gactcgatca
     7381 gccacaaagc cgcctcgggg gcgaaggtaa tctctagttt cgcgccaagc atcatctaaa
     7441 atcgtgggaa atctaacggc tgcccacttg cgacgtcaag cgcaaccgcg gacagcgcaa
     7501 cctcagataa aagcaagcta ggacacgcaa ttcttgataa ccgctcaaga tggagacatg
     7561 cggcaccttt cccagtgcgc gaaagctgtc aacggcataa tttcccgccg aacggttttc
     7621 tttgtccaaa tcagcacggt cccataggaa accctgctgt gttctccatc acgtgcttcg
     7681 ggaaagatcg tttaaaaatc ggcgccgcac cgcatgcgcc cctgcgtcac atcagcgcaa
     7741 gaagagcagg gcagcatcgt cggcgaagcg aaggccttgg ggggtaaggt aaaaacggtc
     7801 ggacagaata cgcccccagc ctcgttgaac caaaagattc atctcttcgc tccattgttg
     7861 ccttaagtca tagccggtaa tctgacggaa ttgatcgaaa ggccagccgc ttggcatgcg
     7921 caaaccaaac gccgccgctt ccccggcgcg tgcaagcggt ggcaattctt cgcggtattc
     7981 aagagctcgc tggccttgct ctaattgttc gcaataacgc cgggtgtcag ggctgttttg
     8041 ggttcgcacg ccacgcacat aagaggtcgc actgggcccc aaaccgtaga agtcgccgcc
     8101 acgccagtaa ttgacgttgt gctggcaagc atgagagggc aggtttgctg tttggggcag
     8161 gcggcttaag gcccgatcgc aggcaaagtt ggcgatttcg tattgcttga ggctggcgtt
     8221 ggtggcctgt tcaagcagtt cttcatacat ggcgcatgcc aggtcttcat ccgccgcggt
     8281 cgggcaagtc tcaagttgct ggtagagcag cgtatcttct tcgtagatca cctcgtaaca
     8341 ggagaggtgt tcgctgccca gggcaaaggc ctcgtgtaag gtttcgcgcc aggcttcaag
     8401 cgtttggccg gggatggcaa acatgagatc caagttcagg ttgtcgaagc cggccttacg
     8461 aaggagatca aaggactgga aaatctcgcg gcgagtatga acccgtccta agcgctgcag
     8521 caacgcttcg ttgaacgatt gcacgcccag agaaaaacga tttacaccgg cgtcgcgcag
     8581 cagttttgcc ttttcgaggc atatagttgc ggggttgcat tctaccgtcc attcagttgt
     8641 cttgcccaag ccaagccgat cccacgcacg caggattctc tgccattgcc gcgaatccaa
     8701 aagcgaaggg gtgccccccc cgaaataaat cgtgcgcggg cgcaaatcat gcgcgattcg
     8761 ttccaactca agtatgagcg ccccaacgta gcgctcaatc agttcgccct tggctggctc
     8821 ggagtaaaag gcgcaataag cacatttctg cacgcagaag ggaacatgaa cataaagacc
     8881 cgctaccggt tcggcatcca accgagtcat gatacgaact ccctctccga acgcagatgc
     8941 caattcccag gtcgaccgtt cggcttaagc ttattcaact tggagggctt gctcgagaaa
     9001 ctggggcaaa tggttcaaaa tcgccgcttc gctgacgagc aatgtcacgc ctcccatttg
     9061 ggtagcctcc tgccattccg cgtcattctc accacagaag gctacaacag gcagatggcg
     9121 agtagtcggg ttttgcttaa ggcgggccag tgcggggcac acgttattct gcttagattt
     9181 caggtcggcc aagaccagca tcggcttggc ctgttcagca cactccacaa gttgatccgc
     9241 attcacaatg acctgaacgc gataattcaa gtcttgcagc cgattcacaa gctgactgcc
     9301 tggcagcaac cgctcataaa acacaagcgc aagaggttgc gtcatgagct tagcttggaa
     9361 taaccaaacg cagaatgcaa ggaattcgca tgggtatccg cggagtgcat cttgacactg
     9421 tccttagttg gaggcccaaa gctcgtccca gtggtggttg cggcgggctt catcgagtgc
     9481 gcagaggttg cggagcccgg gttaggtcca gaactgcccg caacgtttga agcgggtttg
     9541 cttctgccga caagccgatt cgaccgcgcc agagccaatc ggccatccgc ggcgggccat
     9601 ccgctggtag cgcatccgtt ccgcatggct ttgaaaggag ttctgctccc gccgaatgat
     9661 ctcgccaact tcgccccggg ggtgccgaaa ccgcgccatc tggcgcaggg ctttttgttc
     9721 ttggccccgg cgcagtttat gccgcagagg tttgaccccg cgtgaaagcg ccggttgggt
     9781 ttcgccgcgt aaggcgcgtc ccaagctcca caggtgctgg ctgccgtgat agaaatccaa
     9841 gagttccagc gccttcggcc accgatcctt tttgaggttc cacacccatt cggctccatc
     9901 gcctaagaac cgcacgttcc gggcacgtcc cagcccttct cgcggggctt cccagtgccg
     9961 ccgctgaccc aaccccatcg ccgggtcctg ccaactaacc cccaccttat cactcaaaat
    10021 gccgcgtccg gcttcggtac gggcggccgc cgcgtggcgg tagaataggc ccaacttcaa
    10081 ttcgtgccct tcgacgcgca gttgctgggt cttcttttta ccccaccccg ctccgcgata
    10141 gcgcactggg caaccgtcga ccatgatcac gcccaactcc gtgcggcccc gccgcgccaa
    10201 acgttcctgc ggcggcggtt cataccggct ccgcgctggg cgctcggcac gggcaccgac
    10261 ccgttgtacc agcgcatgcc gtgtcgcgtc gtctatgccg cccgcccatc ggctggcccc
    10321 ctgcgcggcc gcctcatccg aaccggtggc cgtcaccgtg aagcaaagct tctcggcgta
    10381 cccaggggtg atcttctgat gcgggcccaa cccccaggcc cgccgcaacg cacaacccca
    10441 ctgacccgta atcggatccc ggccataatc aaccctcagt tttaccgcgc ccagttcgcg
    10501 gcgcagggta acgggcctgc gccgtcgctg ctggaggggg aaaaacctcg ccgctcctgt
    10561 cggcgagtgc ttgcagacgc tcctccagtc gttggcgtcc ccattcgcgg gcttccgcca
    10621 cgaccgctcg ttcgaggtcc gccagacggg gaaacggttg ggatattgac gatgttggtt
    10681 tcatataatg actcggcttg catcgttgcg ttatgccggt cgaggaaccg ttacacctct
    10741 agcttaagga cagtgtcaag atgcacccct agccggaacg attcagttga acacgcataa
    10801 ttgactggtt ttgggtgttc aagc
//
LOCUS       DAOPQG010000118        10554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7159_length_10554_cov_16.201353, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000118 DAOPQG010000000
VERSION     DAOPQG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7159_length_10554_cov_16.201353"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..1951)
                     /locus_tag="PKI07_11715"
     CDS             complement(263..1951)
                     /locus_tag="PKI07_11715"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOC51752.1"
                     /translation="MKPFAPLLLALVCTLIPPGRLAAAEKATEFRRLSLTEYRDKMKA
                     GWIGQIAGVTWGAPTEFKFRDRIIPADKVPVWKPAMINDAFGQDDLYVEMTFLRTLEQ
                     YGLNVSIRQAGLDFANSGYPLWCANRAGRDNLRAGIAPPDSGHPKFNQCPNDIDYQIE
                     ADYSGLIAPGLPQFAIEMGEKFGRLMNYGDGMYAGQFIGAMYAAAFFESDPVTIAETA
                     LRAIPADCQYAEMVRDLVAWYRADPNDWEKAWQLCQRKYREDPEYQKASNGGIDCKIN
                     GAYVLLGLLWGQRDPDRTIIISMRGGQDSDCNPSSAAGVLFTTIGFANLPPRFTQELN
                     QKTLFDHTAYNFPKLVAVCEKLARQVVKSAGGKITRRKGEEFFLIPVRAPQPSKLELS
                     WAPGPIANSRFTPAEMAQIRVDNVPAHMHAAVRKFAPGWQIANCGLDMDPGLRAEWGG
                     KPNVLATHPLDKDTGCTLSNRVKVPAGKTARLRLVVGHDPQGDFDLIVRANGRELLRQ
                     PVSKATATRDPWLVREVDLSQFAGKKAPQIELVNQPSGWAFEAAYWAEIKVIIE"
     gene            complement(2007..2984)
                     /locus_tag="PKI07_11720"
     CDS             complement(2007..2984)
                     /locus_tag="PKI07_11720"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOC51753.1"
                     /translation="MSLWLRSPAAPARRGLAGLLCLAGCALAAPPPEPVQPPPPRSRM
                     EVERVLAKAQSTNAAAPAGPMTILLLAGAQDHGAEAHDYPRWQERWARLLGGSAAWGK
                     GPVNLFGETALPPAETPEAPGVRVLKAQAWPRPEQFAAADVLVAYCYLDWDAPRLQQV
                     RRHLARGKGLVVIHSATWTKPRPSAEVGALTGIGGFEFYRHGPVRLRLKQPGHPICRG
                     LPRQIELADETYWPATPPPSAPAYTVLASSAEPVKPGAEVTQPQVMFWTCEPGNGRVF
                     GCVPGHFTWTFDDPYVRLLLLRGIAWAGRSDPNRFDALALPGARLREGD"
     gene            complement(2990..3838)
                     /locus_tag="PKI07_11725"
     CDS             complement(2990..3838)
                     /locus_tag="PKI07_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC51754.1"
                     /translation="MSKIHIGLNAEFSRSSDKPFTWAVQKAAEMGYTYFEPMVHWGRE
                     LMSEAGYFHTVSLFDDPWRIREACDQAGLKISGLQSHGPLGRPEVHGEYLKLAIRAAA
                     EIGVPVVNTDEGIKAPWTTEAEDFVLIKYTLQEAAFVAERRGVKIGLEPHAQYSRHPD
                     GLDRIYHLVKSPAIGINFDTGNAYLCGHDVYAWLERVAPRLVHLHAKDISVSHAEAER
                     GKVTGTPVGCACGEGVLDWARIVDIVRRKCPRDIVFSVECGTPAQAARSLEHLSAVLR
                     AAPEPN"
     gene            complement(3889..5307)
                     /locus_tag="PKI07_11730"
     CDS             complement(3889..5307)
                     /locus_tag="PKI07_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51755.1"
                     /translation="MTPHPHHYPMQKSIPTIPLKPTALTRRDFLKSSAAAVAALSLPT
                     FIPASAFGAGNRIALGCIGVGGMGVGNMKSFLGLDDCRVVAVCDVYADRRRKAKELAD
                     AHYGDRGCEMYADYRSLIARPDIDAVMIAAQDHWHALMATAAAAAGKDLYCEKPLGVS
                     VEQGQQIRRAVRKHRRVFQAGTWQRSQGKFRQACALVRNGYLGQLREIQVAAPGPEYQ
                     PKYAGSLEPQPVPAGFDWEMWRGPAPAQPYNPGRVAWPDWYLIWDYCAGFICNWGVHY
                     LDIAAWGCPALAREACEIQCQGVYRKQGFTDNLERWQATFTFASGLRMRFTDCSSERE
                     LQTGTRFIGDQGWVHVSRAGISAEPQSLLRVLLRPGETHLYPVTGPGEDVVLIKKETG
                     DRAAFKDLNHGQNFLDCIRSRQDPISDVDATHVASYLGLLAEIAARLETRLKWDPVKE
                     KFIGNREANRRLRRPLYNGWKL"
     gene            complement(5320..7524)
                     /locus_tag="PKI07_11735"
     CDS             complement(5320..7524)
                     /locus_tag="PKI07_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51756.1"
                     /translation="MKRPEFNASGGRGSTPGRRVRARAEWPHFAGLVLFLALAASAAP
                     GGLPQPAHPAGRGRVFLAGAAAVDVTPTNLPVIVNGGFFTQTADQVHDRLHVRWLVLD
                     DGRARVALGVLDTCLIPVEFADAVRARAQQLAGIPAERIMLSAVHTHSTPSLMRCLGT
                     DADPHYPAFALPRIVDGLQRAVANLAPARVGWTVAAAPEHTHTRVWIRRPDKRLPDPF
                     GELTVRANMHPGHQNPDTIGPSGPSDPALTLLAVQSPAGRPLAVLANYSMHYFGAPAL
                     SADYYGVFAEKLGTLLGADGAGPAFVGIMSQGTSGDQQWMDYSQPRREITRDAYAGEL
                     AQIAAEAYRKMVFHDWAPLAMRQKTLRLATRQPDAKRLAWAREMAAQLDGRPPRSLPE
                     VYAREQLWLHENPVRAVPLQALRVGELGIAIWPCEVFALSGLKLKAQSPLQPTMNIEL
                     ANAEEGYIPPPELHVLGGYNTWACRTAGLEPQAEPKILAALLGLLEKVSGQPRRPLTV
                     TDGAYAQAVRAGKPLAYWRLEEPGGPAARNAVGARWPAVYEPGVVRWLEGPPSPAFSG
                     RDAINRCPHFAGGRLRAKLKPPGRNSTVEFWFWNGLPHDARPVTGYLYGRGGEALALN
                     GTAGAAGTLAYGPLTGRTVIAPKTWHHVALVREDRRVAVYLDGNPDPELTGEAAPDPS
                     REVFFGGRSDLEATLEGRIDEIAIYPRVLPPAEIHHHYRLGRAATRAVAQTP"
     gene            complement(7718..8842)
                     /locus_tag="PKI07_11740"
     CDS             complement(7718..8842)
                     /locus_tag="PKI07_11740"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOC51757.1"
                     /translation="MSASPPQHDLPSVAGQFQIYGDFCEAAPYGSGHINDTYAVVFNQ
                     SGRPMRYLFQRLNARVFQDPAGLMDNVARVTAHIRRQLEAAGADPVSRRVLTLVPTRA
                     GQCFYADAEGHHWRCYLFIEQARSYDWVETPRQAFEAARAFGRFQQQLADLPAPRLRD
                     TIPNFHHTRSRFDALCRALARDPCNRAAGVKADIEFALRREPIVDVLAGLHARGLLPE
                     RVTHNDCKLNNVLLDDATGEGVCVIDLDTVMPGLVLYDFGDMCRTATCPTAEDERDLA
                     RVEMRLDMFAALVNGYLDSAGGFLNPVEKDHLVFCARLITFEIGIRFLTDHLEGDRYF
                     KVHRPGHSADRARVQFKMVDSFERHEEAMRKVVEAARRAG"
     gene            complement(8852..9766)
                     /locus_tag="PKI07_11745"
     CDS             complement(8852..9766)
                     /locus_tag="PKI07_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOC51758.1"
                     /translation="MSTAPALVVLAAGIGSRYGGLKQIEPVGPAGEAVLDYAVFDAQR
                     AGFGKFIFVIRREIEQDFRDRFGRRFARHVSLDYAFQQLEMLPPGFGVPPGRQKPWGT
                     GHAVLCAAPLLDRPFAVINADDFYGAESFRVLARFLAAPGPAASDRFAMVGFQLDRTL
                     SEHGTVARGLCQADAEGYLVSVEELTAIERQPGGARNREPDGHYRPLTGREIVSMNCW
                     GFTPELLDGLRRLFAEFLARHQGDLKAEFYLPTAVNALIQEGRARVKVLPTPCAWFGV
                     TYREDRAVVTESIRALVRAGEYPERLWG"
     gene            9925..>10554
                     /locus_tag="PKI07_11750"
     CDS             9925..>10554
                     /locus_tag="PKI07_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOC51759.1"
                     /translation="MAAATIGKLTFGAAAFEVVISDLLQEPVECIVNAANSELVHGGG
                     VAAAIRQAAGSELDQECRQRIRAHGRIPVGEAVVTTAGRLPFKGVIHAVGPRFGVGDE
                     ENKLVQTLKNAFLRAHERGWKSLAFPAVSSGIFSVPHDICARAYLRAVREFFTLYPQS
                     PLQTIRLALFPGPLAEHLRQRVPLEGRRRRMPKAGAALTPIPNAPRVLVT"
BASE COUNT         1741 a         3640 c         3493 g         1680 t
ORIGIN      
        1 cccagtggct taccatgacc cgctgctttt tgcagaactt ttaggacact accgcaaaac
       61 gattggcgaa gcgccatcct ggccgtgcgg gagcctcgcg ggttcaatca atccgcgccg
      121 gggagcacgc gttgattcgc gcgcgttttc ccttgaattt ggccccgccg ggaagccgct
      181 gcgaggcccg gcttcggcgc ggcctggccc gctgcggatt agcccggcca cccttattcg
      241 gggacggctt cttcgcgggg cgctattcga taataacctt gatctcagcc caataggcgg
      301 cctcgaaggc ccagccggag ggctggttca ccagttcaat ttgcggggcc ttcttgccgg
      361 cgaactggga gagatccact tcccgcacca gccacggatc ccgggtggcg gttgccttgc
      421 tcacgggctg gcgcagcaat tcccggccgt tggcgcgcac gatgaggtcg aaatcccctt
      481 gcgggtcgtg gccgacgacc aggcgcagcc gcgccgtttt gccggcgggc actttgacgc
      541 ggttgctcag cgtgcagccg gtgtccttgt cgagcgggtg cgtggccagg acattcggct
      601 tgccgcccca ctccgcgcgc aacccggggt ccatatccag gccgcagttg gcgatctgcc
      661 agcccggggc gaattttcgg acagcggcat gcatgtgggc gggcacgtta tccacccgga
      721 tttgggccat ctccgcgggc gtaaagcggc tgttggcaat cgggccgggc gcccaactga
      781 gttccagctt gctgggttgc ggcgcgcgca ccgggatgag gaaaaactcc tcgcctttcc
      841 ggcgcgtgat tttgccgccg gcgctcttga cgacctggcg ggcgagtttt tcgcacacag
      901 ccaccaattt ggggaagttg tacgcggtgt gatcgaagag agtcttctgg ttcagttcct
      961 gggtgaagcg tggcggcagg ttcgcaaagc cgatggtggt gaagagcacc cccgcggcac
     1021 tggaagggtt gcaatccgaa tcctgtccgc cacgcatcga gatgatgatc gtgcggtcgg
     1081 gatcgcgctg gccccacagc aggccgagca ggacgtaggc gccgttaatc ttgcagtcaa
     1141 tgccgccgtt ggaggctttc tgatattccg ggtcctcgcg gtatttccgc tggcaaagct
     1201 gccaggcctt ttcccagtcg ttggggtcgg cacgatacca ggcaaccagg tcgcggacca
     1261 tttcggcgta ctgacagtcc gccggaatgg cccggagggc ggtttcggcg atcgtcaccg
     1321 ggtcgctctc gaagaatgcg gccgcgtaca tcgcgccgat gaattgcccc gcatacatgc
     1381 cgtcgccgta gttcatcagg cggccgaact tttcccccat ctcaatggca aactgcggca
     1441 ggccgggcgc gatcagcccc gagtaatccg cctcgatctg gtaatcaatg tcattcgggc
     1501 actggttgaa cttcgggtgg ccggaatccg gcggcgcgat gccggcgcgc agattatccc
     1561 ggccggcccg gttcgcgcac cagagcgggt agccgctgtt ggcaaaatcc agacccgcct
     1621 gccgaatgga aacgttcagg ccgtattgtt cgagcgtgcg caggaacgtc atctcgacgt
     1681 agagatcatc ctggccgaac gcgtcgttaa tcatcgcggg cttccaaacc gggaccttgt
     1741 cggcggggat gattcgatcc cggaacttga actccgtcgg ggcgccccac gtcacccccg
     1801 cgatctgccc gatccagccg gctttcatct tgtcccggta ttccgtcagc gacaggcggc
     1861 gaaattccgt cgccttctcc gccgcggcca gccggcccgg cgggatgagg gtgcaaacca
     1921 aagcgagcag cagcggagcg aacggtttca tgccgtcatc gtggatgaaa atagcgccgg
     1981 tgtaaatggg aaagcgcggg acacccttag tccccctcgc ggagccgggc cccgggcagg
     2041 gccagcgcat caaagcggtt ggggtcagac cggccggccc aggcgatgcc ccgcagcagc
     2101 aggagccgaa catagggatc gtcgaaggtc caggtaaaat gcccgggcac acagccaaaa
     2161 acgcggccgt tgccgggttc gcaagtccag aacatgactt gcggctgcgt cacctccgca
     2221 cccggcttga cgggctcggc agaactggcc agcacggtgt aagccggcgc gctgggcggg
     2281 ggcgtggccg gccaataggt ctcatcggcc aactcgattt ggcgcggcag gccccggcaa
     2341 atcggatgcc cgggctgctt cagccggagc cggaccgggc catgccgata aaactcaaag
     2401 ccgccgattc cagtcagcgc gcccacttcg gcggagggcc ggggtttggt ccaggtggcg
     2461 gaatgaatga ccaccaaccc tttgccgcgc gcgaggtgtc ggcggacctg ctgcaggcgc
     2521 ggcgcatccc aatccagata acagtacgcc acgagaacat ccgccgccgc gaattgttcc
     2581 gggcggggcc acgcctgggc tttaagcacg cgaacgcccg gcgcctcggg cgtttcggcg
     2641 ggcggcaaag ccgtttcgcc aaacaggttc acggggcctt tgccccacgc cgccgaccct
     2701 cccagcaggc gcgcccacct ttcctgccac cgcgggtagt cgtgggcctc cgcgccgtgg
     2761 tcctgcgcgc cggccagcag cagaatggtc atcgggccgg cgggcgccgc cgcgttcgtg
     2821 ctttgcgcct tcgccaggac gcgctcgact tccattcggc tgcgcggcgg cggcggctgg
     2881 accggttccg gcggcggcgc ggccagggcg cacccggcca ggcacagcag ccccgcaagg
     2941 ccccggcgcg cgggggctgc gggggatcga agccacaagg acactgccgt cagttcggct
     3001 ccggggcggc ccggagcacc gccgaaagat gttcgaggct ccgggcggcc tgcgccggcg
     3061 tcccgcactc aaccgagaaa acaatatcgc gcgggcattt ccggcggaca atatccacaa
     3121 tccgcgccca atcgagcacg ccttcgccgc aggcgcagcc gacgggcgtt cccgtcacct
     3181 tgccgcgttc ggcttccgcg tgcgacacgg aaatatcttt cgcatggaga tgcaccagcc
     3241 gcggcgccac ccgctccagc cacgcataca cgtcgtgccc gcagagatag gcattcccgg
     3301 tgtcgaagtt gatgccgatg gcaggcgatt tgacgaggtg atagatgcgg tccaggccgt
     3361 ccgggtgccg ggaatactgg gcgtgcggct ccaggccgat tttgacgccc cgccgctcgg
     3421 caacgaacgc cgcctcctgc agcgtgtatt tgatcagcac gaagtcttcc gcctccgtcg
     3481 tccagggcgc cttgatgcct tcgtcggtgt tgacgaccgg gacgccgatt tccgccgcgg
     3541 cccggatggc gagcttgagg tattcgccat gcacctcggg gcggcccagc ggcccgtgcg
     3601 attgcaggcc cgagatcttc agccccgcct ggtcgcacgc ttcccggatg cgccagggat
     3661 catcaaacag cgaaaccgtg tggaaatacc cggcttcgct catcagttcc cggccccagt
     3721 ggaccatggg ttcgaaatag gtgtagccca tctccgccgc cttctggacg gcccacgtga
     3781 atggtttgtc cgagctgcgc gagaattccg cgttcagtcc aatatgtatc ttgctcatct
     3841 tcaggtttct ttatgttcgg gttttcaggg gtgcggcccc ggcgcggctc agagcttcca
     3901 gccgttgtac aacggccggc ggagccgccg gttcgcctcg cgattgccga tgaacttctc
     3961 cttcaccggg tcccacttaa gccgggtctc cagccgcgcc gcgatctcgg ccaggaggcc
     4021 caaatacgac gccacgtgcg ttgcgtccac gtccgagatg ggatcctggc ggctgcgaat
     4081 gcagtccagg aaattctggc cgtggttgag gtctttgaac gccgcgcggt cgccggtttc
     4141 ctttttgatc aacacgacat cctcgccggg gccggtcacc gggtacaggt gcgtttcgcc
     4201 ggggcggagc aggacccgca gcagcgattg cggctcggcg gaaatgccgg cgcggctgac
     4261 gtgcacccat ccctggtcgc cgatgaaccg cgtgccggtc tggagctccc gctcgctgct
     4321 gcagtcagtg aagcgcatcc gcaagccgct ggcgaacgtg aaggtggcct gccaccgttc
     4381 gaggttgtcg gtgaagccct gcttgcgata aacgccctga cactggattt cacacgcctc
     4441 ccgcgccagc gccgggcagc cccaggcggc gatatcgaga taatgcacgc cccagttgca
     4501 aataaagccg gcgcagtagt cccagatcag ataccagtcc ggccaggcaa cgcgcccggg
     4561 attatagggc tgggccggcg ccgggccgcg ccacatctcc cagtcgaagc cggccggcac
     4621 cggctgcggt tcgagggacc cggcatattt gggctggtat tccgggccgg gagcggcgac
     4681 ctggatctcg cgcagttggc caaggtagcc gttgcggacc agcgcgcagg cctgccggaa
     4741 cttcccctgc gaccgctgcc acgtgcccgc ctggaacacg cgccggtgct tccgcacggc
     4801 ccggcgaatc tgctgcccct gctcgaccga tacgcccagg ggcttctcgc aataaagatc
     4861 cttgccggcc gccgccgccg ccgtcgccat cagcgcgtgc caatggtcct gcgcggcgat
     4921 catcaccgcg tcaatatccg gccgggcgat taaggagcgg tagtcggcat acatctcgca
     4981 gccccggtcg ccgtaatgcg catccgccag ctctttggct tttcggcgcc ggtccgcgta
     5041 aacgtcgcaa accgccacga cccggcaatc gtccaggccc agaaagctct tcatattgcc
     5101 gacgcccatt ccgccgacgc cgatgcagcc cagcgcgatc cggttgccgg cgccgaacgc
     5161 cgaggcggga atgaaagtgg gcaggctgag tgccgcgacg gccgccgccg aacttttcag
     5221 aaagtcgcgg cgggtgaggg cggtgggctt caagggaatg gtggggatgg atttttgcat
     5281 ggggtagtga tggggatgcg gtgtcatggg ccaggcggtt tacggggtct gggcgacggc
     5341 gcgggtcgcg gcgcggccca gccggtaatg gtgatggatt tccgcgggcg ggagcacccg
     5401 cgggtagatg gcgatttcgt caatgcgccc ctcgagcgtg gcctcgaggt cgctccggcc
     5461 cccgaagaaa acctcccggg atgggtccgg cgcggcctcg cccgtcagct ccggatcggg
     5521 attgccgtcc aggtaaaccg ccacccgccg gtcctcccgc accagcgcca cgtggtgcca
     5581 cgttttcgga gcgatgaccg tgcggcccgt cagcgggcca tacgccagcg tgccggcggc
     5641 gccggcggtt ccgttgagcg caagcgcctc gccgccgcgc ccgtacaggt agcccgtgac
     5701 cggccgcgcg tcgtggggca ggccgttcca gaaccagaac tccaccgtgc tgttccgccc
     5761 cggcggcttc agcttcgcgc gcagccggcc gccggcgaaa tgcgggcaac ggttaatcgc
     5821 gtcccgcccg gaaaaagccg gcgacggcgg cccctccagc cagcggacaa cgcccggctc
     5881 atagacggcc ggccagcgcg cgccgacggc gttccgcgcg gccggcccgc cgggttcctc
     5941 cagccgccag taggccagcg gtttgcccgc ccgcaccgcc tgcgcgtagg cgccgtcggt
     6001 gaccgtgagc gggcggcggg gctggccgct gaccttttcc agcagcccca gcagcgccgc
     6061 caagatcttg ggttcggcct ggggttccag gccggcggtg cggcaggccc aggtattgta
     6121 tccgcccagc acatgcagct cgggcggagg gatgtagccc tcctccgcgt tggccagctc
     6181 gatgttcatc gtcggctgga gcgggctttg ggctttgagc ttcagtccgc tgagcgcgaa
     6241 cacttcgcag ggccaaatgg cgatgcccag ctccccgacg cgcaaggcct gcaagggcac
     6301 ggcgcggacc gggttctcgt gcagccagag ttgctcgcgc gcataaacct ccggcagcga
     6361 ccgcggcggg cgtccgtcga gttgggcggc catctcccgc gcccaggcca gccgcttcgc
     6421 atccggctgg cgcgtcgcca gccgcagcgt tttctggcgc atcgccagcg gagcccagtc
     6481 atggaacacc atcttccggt atgcctccgc cgctatttgc gccagctcgc ccgcgtaagc
     6541 gtcccgggtg atctcgcgcc gcggctggct gtaatccatc cactgctgat cgccgctggt
     6601 gccttgcgac atgatgccca caaaggccgg gcccgctccg tccgcgccga gcagcgtccc
     6661 cagcttttcc gcaaacactc cgtaatagtc cgccgagagc gcgggggcgc caaaatagtg
     6721 catcgaatag ttcgccagca cggcgagggg ccgtcccgcg ggggattgca ccgccagcag
     6781 cgtcaacgcc gggtcggacg gccccgacgg cccgatggtg tccgggttct ggtggccggg
     6841 atgcatattg gcgcgaaccg tcagctcgcc aaacggatcg ggcagccgtt tgtctgggcg
     6901 gcgaatccac acccgcgtgt gtgtatgctc cggcgccgcc gcgacggtcc agccgacgcg
     6961 ggccggggcg agattcgcca ccgcgcgctg caaaccgtcc acgatgcggg gcagcgcaaa
     7021 cgccgggtaa tgcgggtcgg cgtcggtccc caggcagcgc atgagcgacg gcgtgctgtg
     7081 tgtatgcacc gccgaaagca tgatgcgctc cgccgggatg cccgcaagct gctgcgcgcg
     7141 cgcccgcacg gcgtcggcga actcgaccgg gatcaggcac gtatccaaca cccccagcgc
     7201 cacccgcgcg cgcccgtcat ccagcaccag ccagcgcaca tgcaggcggt cgtgaacctg
     7261 atccgccgtc tgggtaaaga agccgccgtt gacaatcacc ggcagattcg tcggcgtcac
     7321 atccaccgcg gccgcacccg caagaaagac ccgcccccgg ccggccggat gcgccggctg
     7381 cggaagcccg cccggcgcgg cgctggccgc cagcgcaagg aaaagaacca gccccgcaaa
     7441 gtgcggccac tccgcccgcg cccggacccg ccgtccgggg gtgctcccgc gtccgccgct
     7501 tgcgttaaac tccgggcgtt tcatggcgcg gcggcccggc ggcgaacggg atcggaagga
     7561 tgcaggttca cgcccccttt ataaagcaat cgggccaaag cgcaattccc aaactcggcg
     7621 cccgccgccg ccgcggccgg acgccgccgg cggcggggcg gggggcggcg ggagcgggct
     7681 tccggcggcc gcggttggcg ggcttcgccg ccgtgcctca acccgcgcgc cgggcggctt
     7741 ccaccacctt gcgcatggct tcctcatggc gctcgaagga gtccaccatc ttgaattgga
     7801 cccgggcgcg gtccgcgctg tggcccggcc gatgcacctt gaagtagcgg tcgccctcca
     7861 ggtgatccgt caggaaacgg atgccgatct cgaaggtgat gagccgggcg cagaagacca
     7921 ggtgatcctt ttccaccggg ttaaggaacc cgcccgcgga gtccaggtag ccgttcacca
     7981 gggccgcgaa catgtccagc cgcatttcaa cccgcgccag gtcgcgctcg tcttccgccg
     8041 tcgggcaggt cgcggtgcgg cacatatcgc cgaaatcata aagcaccagc cccggcatca
     8101 cggtgtccag atcaatcacg cagacgccct cgccggtggc gtcatccagc aggacgttgt
     8161 tcagcttgca gtcgttgtgc gtgacccgct cgggaagcag gccgcgcgcg tgcaggccgg
     8221 ccagcacgtc cacgatgggc tcgcggcgca gggcaaactc gatatccgct ttcacgccgg
     8281 cggcgcggtt gcagggatcc cgcgcgaggg cccggcacag cgcgtcgaac cggctgcggg
     8341 tgtggtggaa gttgggaatg gtgtcgcgca ggcggggcgc gggcaggtcg gcgagttgtt
     8401 gctggaaccg gccgaaggcc cgggcggcct cgaacgcctg ccggggcgtc tcgacccagt
     8461 cgtagctccg ggcctgctca ataaaaagat aacagcgcca gtggtggccc tcggcgtcgg
     8521 cgtaaaagca ttggcccgcg cgcgtcggca ccagggtcag cacccggcgg gaaaccgggt
     8581 cggcgccggc ggcctccaat tggcgccgga tgtgggccgt gacgcgcgcc acgttatcca
     8641 tcagcccggc cgggtcttga aagacgcgcg cgttgaggcg ctggaacagg tagcgcatcg
     8701 ggcggccgga ctggttaaac accaccgcgt aagtgtcgtt gatatggccg ctcccgtagg
     8761 gggcggcttc gcaaaaatcg ccgtaaatct ggaattgccc ggcgaccgac ggcaggtcgt
     8821 gctggggcgg ggaagcggac atggaaccgg ctcagcccca aaggcgttcc gggtactcgc
     8881 cggcgcggac cagcgcccgg atgctttccg tgaccaccgc gcggtcttcc cggtaagtga
     8941 cgccgaacca ggcgcagggc gtcggcagca ccttcacgcg cgcccggcct tcctggatca
     9001 aggcattcac cgccgtcggc aggtagaact cggctttgag atccccctga tgccgcgcca
     9061 aaaactccgc aaagaggcgt cgcaagccgt ccagcagctc cggcgtgaac ccccagcaat
     9121 tcatcgagac aatctcgcgc ccggtcagcg gccggtaatg gccgtccggc tcccggttcc
     9181 gggcgccgcc gggctggcgt tcgatggcgg tgagctcctc caccgacacg aggtaaccct
     9241 ccgcgtcggc ctggcaaagg ccgcgcgcca ccgtgccgtg ctccgaaagc gtgcggtcca
     9301 gttggaagcc caccatcgcg aaccggtcgg acgccgccgg ccccggcgcc gccaggaacc
     9361 gggcgagcac gcgaaacgac tccgccccat agaaatcatc cgcattgatg accgcaaacg
     9421 gccggtccag cagcggcgcg gcgcacagaa cggcatggcc ggtgccccac ggtttctggc
     9481 gtccgggcgg cacgccgaac ccgggcggca gcatttcaag ctgctgaaaa gcgtaatcca
     9541 ggctgacgtg ccgggcaaaa cggcggccga aacggtcccg gaagtcctgc tcaatctccc
     9601 ggcggatcac aaagataaac ttgccaaaac cggcccgctg cgcgtcgaac acggcataat
     9661 ccagcaccgc ctcgccggcg ggaccgacag gctcaatttg cttcaaaccg ccataacggg
     9721 aaccgatgcc ggcggctaaa acgaccaatg caggtgcggt gctcaaactc gaccgcagac
     9781 gctaccgtcc ccccggcgcg gtgcaagccc aaaaagcggc ggcgcggcgt caaaaacgcg
     9841 ccccgccgtc cgcgccccct cccgggaacc ggcccgggtc tggcttgcgc cggggcgcat
     9901 aatgtgttcc gctgggcggc ggatatggct gctgcaacca tcggcaaact cactttcggc
     9961 gccgccgcgt tcgaggtcgt catcagcgac ctgctccagg aaccggtgga gtgcatcgtc
    10021 aacgcggcca acagcgaact cgtgcatggc gggggagtgg cggccgccat ccggcaagcc
    10081 gccgggagcg aactcgacca ggaatgccgg caacgaatcc gcgctcacgg ccgcatcccc
    10141 gtgggagaag ccgtcgtgac gaccgccggc cggctgccct ttaagggcgt catccatgcc
    10201 gtgggccccc gctttggcgt gggcgacgag gagaacaaac tcgtgcaaac gctcaaaaat
    10261 gcctttctgc gcgcgcacga acgaggctgg aaatcgctcg cctttcccgc cgtcagttcc
    10321 ggcatcttct ccgtgccgca cgacatctgc gcccgggcct acctccgcgc cgtgcgcgaa
    10381 ttcttcaccc tatacccgca atcgcccctc caaaccatcc gcctcgcctt gttcccgggc
    10441 cccctggccg aacacctgcg ccaacgtgtt cccctcgaag ggcgccgacg aaggatgccc
    10501 aaagccgggg cagccttaac ccccatccca aacgcgcctc gcgtcctcgt caca
//
LOCUS       DAOPQG010000119        10527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7177_length_10527_cov_14.259167, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000119 DAOPQG010000000
VERSION     DAOPQG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10527
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7177_length_10527_cov_14.259167"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..177
                     /locus_tag="PKI07_11755"
                     /pseudo
     CDS             55..177
                     /locus_tag="PKI07_11755"
                     /inference="COORDINATES: protein motif:HMM:NF025596.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            316..738
                     /locus_tag="PKI07_11760"
     CDS             316..738
                     /locus_tag="PKI07_11760"
                     /inference="COORDINATES: protein motif:HMM:NF025180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HOC51760.1"
                     /translation="MISLLQLLSHAPAPRDTNTHYRIGPVLTLIAMARLAGRREIAAI
                     ARFATTLTQRQRHRLGLPLKKGTRNFHKVPGYDVFYQVLTRMNPETFATLLSTWPCRQ
                     AGQLPQALALDGKMIRDQIGLLTLAQHEDGAPQAGHGL"
     gene            946..1089
                     /locus_tag="PKI07_11765"
                     /pseudo
     CDS             946..1089
                     /locus_tag="PKI07_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE transposase family protein"
     gene            1441..2484
                     /locus_tag="PKI07_11770"
     CDS             1441..2484
                     /locus_tag="PKI07_11770"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC51761.1"
                     /translation="MDIGMVPRLTIVGSIGATHQIQYCTNLNQPQWIVLTNLLVSESP
                     CRFVDMSAPAASRRFYRVTKLSPDGMVLIPAGSFSMGDGSDGNTPSLPVHTVYVSEFF
                     MDRYEVTKALWDTVKAYNGGNGYSYENAGAGKAANHPVHSVNWRDCVKWCNARSQREG
                     RTPCYYNDAGLTSVYKSGTNAPFVKWNANGYRLPTEAEWEKAARGGASGQRFLWGNTI
                     SWSQANYRAFPLSAGGYVYDINATAGYTPAFNDGVAPYTSPVGSFAANGYGLHDMAGN
                     VWEWCWDWYGNYAAGSQNDPTGPATGTCRTFRGGSWFSDARDARCAQRYGFDPSGKFD
                     TFGFRTVISACQP"
     gene            complement(2534..3907)
                     /gene="prsR"
                     /locus_tag="PKI07_11775"
     CDS             complement(2534..3907)
                     /gene="prsR"
                     /locus_tag="PKI07_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HOC51762.1"
                     /translation="MPRLLIVDDDEEIRTQMKWALGKEYQIFLAEDRVRAIDAFMEHR
                     PQVVLLDLGLPPHRGTPEEGLAVLSELIARDRFAKIIIVSGQAEKTNALQAIGAGAYD
                     FLTKPVDVEELKIILRRTFHVAGLERDYRKLQAQLQTETFEGLLGTSPQIQRVFASVR
                     KVAATNAPVLILGESGTGKEMLAQAIHRLSSRKDGPFVAINCSAIPETLLESELFGHE
                     KGSFTGAHVQRNGRIEVASGGTLFLDEIGEIPQQIQVKLLRFLQEQRIERVGGRQEIQ
                     VDTRVVAATNVDLKKAISTERFREDLYYRLAVVVIHLPPLRDREGDIRLLAQEFLRRS
                     TVANEKEGISFSQDALRAILAHSWPGNVRELENRIKRAVIMAEGKRISAADLELESPA
                     RTVNLNLKDAREAVEREVVQRALRKHGGRIAPAAAEMGISRPTLYELMEKLGIEKAER
                     EEDKEPA"
     gene            complement(3931..6030)
                     /gene="prsK"
                     /locus_tag="PKI07_11780"
     CDS             complement(3931..6030)
                     /gene="prsK"
                     /locus_tag="PKI07_11780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HOC51763.1"
                     /translation="MTTGAALNFASVFSAGLLALTIFTRQFRSFPRWAFSAGMLAFAA
                     ESLFAGLAARAVPLEAVERWQNLAFVAMAFLPGLWLLFSLTYARGNSGEFLSRWSVVV
                     ACAFIVPLILVLLCPDEIISAIRQPPDRSQRVLRLGFAGSALNLIFLLGTILVLMNLE
                     RTFRAAVGTMRWRIKFVILGLGVLFTVRAYTSSQALLYRGGDLSLPAVNAGALLLASL
                     LILRGLLRAGHSDVSIYPSHSLLHHSFTVLLAGVYLLVVGVFAKVVTHFGGDAAFPIK
                     AFLVLLFLVLLAVLLLSDRVRLFTRRFVSRHFSRPVYDYRSVWRTFTESTARRVEQNE
                     LCSSVVKLVSDIFQALSVSIWLVDERKDQLSFAASTSLSQQKAGQLALDAADAAEVIS
                     ALSAHPDPVDLDSSKAHWAAALRRSHPDEFRKGGSRVCVPLIAGGEMLGVLVLGDRVG
                     AVPFSVQDFDLLKSIGEQSAANLLNIQLSQRLSQAKQLEAFQAMSAFFIHDLKNTAST
                     LSLMLQNLPVHYQDPQFREDALRGISKTVTRINETISRLNELRHELALQPVECDLNDL
                     ITETLKGQEQAADISLLKELAPLPKVRLDPAQIQKVITNLVLNARDAVSSSGQIRVAT
                     SQENGWVILVVADNGCGMTPEFIQRSLFRPFHTTKQKGIGIGMFHCKMIVEAHRGRIE
                     VQSEPGKGTRFRVLLPI"
     gene            complement(6138..6710)
                     /locus_tag="PKI07_11785"
     CDS             complement(6138..6710)
                     /locus_tag="PKI07_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPDSG-CTERM sorting domain-containing protein"
                     /protein_id="HOC51764.1"
                     /translation="MKSNIVKYFAVAVAAFGLAISAHAVKITGEINMAGSVTLDSSWL
                     GTANGVTGFGPVVVGVAPTGDFAGTAGASVSWSTFSWNPPSTPVIPLWTFTSGPLTYS
                     FDLLSLSVAQQDNSFLNLIGWGTLKITGFEDTVGTWSFTIPNAGGGQHANFDFTFANS
                     QNAVVPDGGMTAMLLGAALSGLALLRRKLA"
     gene            7853..9934
                     /locus_tag="PKI07_11790"
     CDS             7853..9934
                     /locus_tag="PKI07_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51765.1"
                     /translation="MHLAKNRLRTILIGVMLGGITLAAFWPVIHNDFIIYDDAAYVTE
                     NPQVLAGLTWRQAGLAFRTSCAGNWHPLTVLSHMLDVQLFGLNPAGHHLTSLLLHAAN
                     AVLLFLVLRRATQPSNPQLPTSRLESRPPPALDPFWLCAMVAGLFALHPLRVESVAWV
                     AERKDVLSAFFFLLTLWAYVRNAEGQTPESTTRAPLYATRFYVLALVFFALALMSKAM
                     VVTLPFILLLLDYWPLRRLQFSSPCWLSTLRRLLLEKLPFFALTALFTGVAFLLLRQA
                     GATQELPGVGWGHRLARIFVGYEQYLAKTIWPVDLALPYPRLAEVSLGLALAGAAAVI
                     SLSFIAIWHGRRRLYAPVGWFWFLGMLIPVIGLVGVGAQTIADRYTYLPSIGLSIVAA
                     WGLGEIAGRPRLRLPMIGASVLALAALLLATHHQLRYWQNSEALFRHALAVTDVNPLA
                     HSLLASVLAEAGELEKAETHCREARRLMPNCAETEILYARVLARRNKFEEAIAVLSQL
                     LRHSSVAPEAHFNLGAIFGEQGAASQAMNSYRQALALKPDFPEAYFGLAALFTQQGDT
                     AAAIEEYYQGLRRQPDAPEALNNLAWIRAASANAAFRNGEEAVRLASRACELSGRQNP
                     VMLGTLAAAYAEAGRFDDAVATAQEACNIALAKGQGDLAKKNQALLELYQSRRPYHES
                     LEPFPASPSHL"
BASE COUNT         2219 a         3181 c         2988 g         2139 t
ORIGIN      
        1 cggcgcttaa gtttcggtag atgagaggga aaagagtttg ggtaccggcc gctgctggct
       61 gagagcttta ccgacccgga gggatacgcc ggcacctgct acaaggccag caactgggag
      121 gcggtgggct ggagcaaggg ctaccgccgg caccgggcgg atttttatct ccctaatgaa
      181 cggcccaagc gcctgtggct gcggtctctg gagccggaag cccgtccgcg gttgcgagcc
      241 cgggaactgc ccgcgccgta tcgggccgcg ttagtggcgg ccccctcggg ccagttgccc
      301 atcaaagcgc cgcaaatgat ctcgctcctc cagctcttga gccatgcccc agccccccgg
      361 gataccaata cccactatcg gattggcccg gtattgaccc tgattgcgat ggcgcggttg
      421 gcgggccggc gcgagattgc cgccatcgcc cgctttgcca ccaccctgac gcaacgccag
      481 cgtcaccggt tgggcttgcc gttgaaaaag gggacccgca acttccataa ggtgccgggg
      541 tatgacgtgt tttatcaggt gcttacgcgc atgaaccccg agacctttgc cacgttgctc
      601 agcacttggc cttgccgcca agcgggccag ttgccgcaag ccctggcgct ggacggcaaa
      661 atgattcggg atcagatcgg gctgctcacc ctggctcagc atgaggacgg agcgccccag
      721 gctggtcacg gtttatgact cgaaagaggg cacggacgcg tcgaaacccg attcctgtat
      781 cccttcgcca ttgaacccct gcacgccgac ttcccctttg cccgcacctt gatcgtgcag
      841 cgcaatcacc gcacgatcaa aaagagtggc caaacgacaa tcgaatcaca cgactatctt
      901 tccagcgtcc cgccggagca ctatcgcccg cttcaatggc tccaactgac tcgggaccac
      961 tgggcggggg tggaaatccg caatcattgg cgccgggatg cgttgatggg cgaggacacc
     1021 tcacgcagtc gcaacccccg cctgctggcg aatctggccc tgttgcgcag cgcccggctg
     1081 gccctgattt gctctccggt tggcatgacc tcacccgtga gtgatttcgg ctcagcccgt
     1141 gcgaaattca cacagcattc cgagcgggat ttaaccggtt tgcctggcaa attaagatca
     1201 acggcaggct tgcgatctgg cagataaact caattcagca tcttactgtt cctcgcgaac
     1261 acagaggccc gctattgacc gcatactggc atggtctatg ctatccgtac tactacctat
     1321 acggttacga aaactaaagc tatgttgacg agactttcaa attccttgcg gcgcctgttt
     1381 gtttggtttg ttgcattagt aggggttggc gcagggacgg gatgggcagt ggagggaatt
     1441 gtggatattg gtatggtgcc ccgcctgact attgtgggca gcatcggtgc cacccatcaa
     1501 atacaatact gtaccaacct gaaccaaccc cagtggatcg tgctgacaaa tctgttggtg
     1561 tcggaaagcc cctgcaggtt cgtggatatg agcgctccgg ccgcatccag aaggttttac
     1621 cgggtgacga aactctcacc ggatgggatg gtgcttattc ccgctggcag cttttccatg
     1681 ggggatggct cggacggaaa tacgccatcc ctccctgtcc atacggtgta tgtgagtgag
     1741 tttttcatgg accgctacga ggtgaccaaa gcactgtggg acacggtgaa ggcctacaat
     1801 gggggcaatg gctacagcta tgaaaatgcc ggggcgggaa aagctgcgaa ccacccggtg
     1861 cattcggtca actggcggga ttgcgtgaag tggtgtaatg cgcggagcca gcgggaaggg
     1921 cggacgcctt gctattacaa cgacgctgga ttgacgagcg tatataagtc cgggacaaat
     1981 gctcccttcg tgaaatggaa tgcgaacggc taccggttgc cgacggaggc ggagtgggaa
     2041 aaggcggcgc ggggaggggc aagcgggcag cgatttctgt ggggcaatac catttcgtgg
     2101 agccaggcga actaccgcgc gtttcccttg tcagcgggag gctacgttta cgacatcaac
     2161 gccacagccg gctatacccc tgcgttcaat gatggggttg ccccctacac cagtcccgtc
     2221 ggcagctttg cggcaaacgg gtatggcttg catgacatgg ccggaaacgt gtgggaatgg
     2281 tgttgggatt ggtacggaaa ctacgcggct ggctctcaga acgatcccac gggtcccgcg
     2341 accggaacgt gccgcacatt tcgcggcggc agttggttta gtgacgctcg ggatgcccga
     2401 tgtgcgcagc gctacggctt cgatccatcc ggcaaatttg atacttttgg ctttcgaacg
     2461 gtcatttccg cctgccagcc ctgacttggg ccatatctga attaaccggc gctggccgga
     2521 ggccaaagcc tgcctaagcg ggttctttgt cctcctcccg ttccgctttc tcgatgccca
     2581 gcttctccat taattcgtag agggtgggcc ggctgattcc catttcggca gcggcgggcg
     2641 cgattctgcc gccatgcttc cgcagcgccc gttgaacgac ttcacgctcg acggcttccc
     2701 gcgcgtcttt gaggttcagg ttaaccgtgc gggccgggct ctccaattcc aggtccgctg
     2761 cggatattcg tttgccttct gccataatga cggcgcgctt gatacggttc tcgagttcac
     2821 gcacgttgcc cggccaggaa tgggccagaa tggcccgaag cgcatcctgg ctgaagctga
     2881 ttccctcttt ctcattggcc acagtgctgc gtcgcaggaa ttcctgcgcc aacaggcgga
     2941 tatcgccttc ccgatcgcgc aacgggggca ggtgaataac cacaaccgcc aggcggtaat
     3001 acaggtcttc ccggaaccgc tcggtgctga tcgctttttt caggtcaacg ttggtggccg
     3061 cgaccacgcg cgtgtcaacc tggatttcct gccgcccccc gacccgctcg atacgttgct
     3121 cctggaggaa ccggagcagc ttgacttgaa tttgctgggg gatttcgccg atttcgtcca
     3181 ggaacagagt gccgccgctg gctacctcaa tgcgcccatt acgctgaaca tgcgcgccgg
     3241 tgaaggagcc tttttcgtga ccaaacagct cgctctccag cagggtctcg ggaatggcac
     3301 tgcagttaat ggcaacgaag gggccgtcct tcctcgaact gagtcggtgg atagcctgcg
     3361 ccagcatttc cttaccggtg ccgctttcgc ccagaatcag gaccggggca ttagtagccg
     3421 cgactttgcg cacggaggcg aaaacccttt ggatttgcgg gctggtgccg agcagcccct
     3481 caaaagtctc cgtctgaagc tgcgcttgca gcttgcgata gtcgcgctcc aacccggcaa
     3541 cgtgaaaggt gcgacggaga ataatcttta actcctcgac gtccacgggc ttggtcagga
     3601 aatcgtaggc ccccgctcca attgcttgca aggcattggt cttttccgcc tggccggaaa
     3661 cgatgatgat tttggcgaag cggtcgcggg cgatcaattc ggagagcacc gccaaccctt
     3721 cttcgggggt accacggtga ggaggaagcc ccaggtccag cagcaccacc tgcgggcggt
     3781 gctccataaa tgcatcaatg gcccgcacgc gatcttccgc caggaaaatc tggtattcct
     3841 tgcccagcgc ccatttcatc tgggtgcgaa tctcctcgtc gtcgtccaca atcagcaatc
     3901 gcggcagcat aattataaat ggcgtattcg tcagattggc aacagcaccc ggaatcgcgt
     3961 tcccttgccc ggctcgctct ggacttcaat gcgcccgcgg tgggcttcca caatcatctt
     4021 gcaatggaac atgccgatgc cgatgccttt ttgtttggtg gtgtgaaagg ggcggaagag
     4081 cgagcgttgg atgaattcgg gagtcatgcc gcaaccatta tccgccacga ccagaatcac
     4141 ccagccgttt tcctggctgg ttgccacgcg gatttggccg ctggaagaaa cggcatcccg
     4201 ggcgttaagc acgaggttgg tgatcacctt ctggatttgc gcggggtcca accggacttt
     4261 gggaagcggg gcaagctctt taagcaggga gatgtccgcc gcttgctcct gccccttaag
     4321 ggtttccgta atcaaatcat tcagatcgca ttccacgggc tggagcgcca actcgtggcg
     4381 caactcgttt agccggctga tggtttcatt gatgcgggta acggtcttgg aaattccccg
     4441 cagcgcgtcc tcgcgaaact ggggatcctg gtaatgtacc ggcagatttt gcagcatcag
     4501 cgacagggtg gaggcggtgt tcttcaagtc atgaatgaag aaagcggaca tcgcctgaaa
     4561 ggcctccaat tgtttggctt gtgaaagccg ctgggataat tggatgttga ggagattggc
     4621 cgcgctttgt tcgccaatgg acttgagcag atcaaaatcc tggaccgaga acggcaccgc
     4681 gcccacacgg tcgccaagga ccagcactcc gagcatctca ccgccggcaa tcaacggaac
     4741 gcaaacgcgg ctgcctcctt tgcggaactc atccgggtgc gaacgccgca gcgccgcggc
     4801 ccagtgcgcc tttgaagaat ccaaatccac cgggtcggga tgggcgctca aggctgagat
     4861 gacttcggcc gcatcggcgg cgtccaacgc cagttgcccg gccttttgct gggaaaggga
     4921 ggtcgaagcg gcaaagctca actgatcctt gcgttcgtcc accagccaaa tagaaaccga
     4981 gagcgcctga aagatatcag aaaccagttt gacgacggaa ctgcacagtt cattctgctc
     5041 aacccgccgg gcggtgcttt cggtaaaggt ccgccaaacg ctgcgataat catagaccgg
     5101 tcgcgagaaa tgacggctga cgaatcggcg ggtaaaaagc cgcacccggt ccgaaagcag
     5161 caaaacggcc aaaagaacca gaaacagcaa aaccagaaag gccttgatcg gaaaggcagc
     5221 gtcgccgccg aaatgagtga ccaccttggc aaacacaccc accaccagca agtaaacccc
     5281 cgctagcaga accgtgaagg aatggtgcag taatgaatga gacggataaa tgctcacgtc
     5341 ggagtggccg gcgcggagga gtccccgcag tatcaggagg ctggcgagca gaagcgcccc
     5401 ggcattgact gccggcaggg aaagatcgcc gccgcgatac aatagtgcct ggctgctggt
     5461 gtaggcgcgg accgtaaaga gcacgcccag gcccaggatg acaaacttga tgcgccagcg
     5521 catagtcccc acggcagcac gaaaggtgcg ctcaagattc atcaacacca gaatagtgcc
     5581 gagcaaaaat atcaaattca gcgccgaccc ggcgaagccc agacgaagca cccgctggct
     5641 gcgatcgggg ggttgtcgaa tggcgctaat gatttcgtcc gggcaaagca ataccaggat
     5701 cagcggaacg atgaaggcgc aggcgaccac gactgaccag cgggagagga actcgcccga
     5761 gttgccgcgg gcataggtaa ggctaaagag cagccaaagt cccggcagaa aagccatcgc
     5821 cacgaaagcc aggttttgcc agcgctccac cgcttcgagc gggacggctc gggcggcgag
     5881 gccagcaaag aggctttcgg ccgcaaaggc cagcattcca gccgaaaagg cccaacgcgg
     5941 gaaagaacgg aactgccgcg taaagatcgt gagcgccagt aatccggcgg agaacacgct
     6001 ggcaaaattc agtgcggctc cggtggtcat tcgccgttaa aatagctgga acaacctttg
     6061 atagaaataa aaaacaggca ggaacctgcg ttcctgcctg cgtagaacag aagaactttc
     6121 aacaaggcgc cgcctgctta ggcaagcttc cggcggagca gcgccagccc cgagagggcc
     6181 gcgccgagga gcatggcggt catcccacca tccggcacca ccgcattctg agagttggca
     6241 aacgtgaagt caaagtttgc gtgctgcccg ccgccggcat tgggaatggt gaaggaccaa
     6301 gtccccaccg tatcctcaaa accggttatc ttcagcgtcc cccagccaat caggttcagg
     6361 aaggaattat cctgttgcgc cacactgagg ctcagcaggt caaagctgta ggtcagcggc
     6421 ccagaggtga acgtccaaag cggaataacc ggcgtgctcg gcggattcca ggagaatgtg
     6481 ctccagctca cactggcacc agcggttccg gcaaaatcac ctgtcggagc aacgcccacc
     6541 actaccggcc caaacccagt caccccattg gcagtcccta accaggagct gtccaaagta
     6601 accgaaccgg ccatgttaat ctcaccagtg atcttgaccg catgcgcgct tatcgccaag
     6661 ccgaacgccg ccaccgccac tgcgaagtat ttcacgatat tactcttcat tctcttagtt
     6721 ctttctattg ttgtcgttgt ttaatctgct ctactacgcc cagcaaatag caactaacgg
     6781 gccaactaaa cacaggcaaa gtattgaatg atataaatta tccattatca gcatgttaca
     6841 tcaaaataaa aacgcaatgt agttgaaaca gcctaagaaa tctcggcgga aatagtaaag
     6901 cttaccgacg gttttgatac ccaaaaatgg ccgatacaga ccagattgta tgcaaagtgc
     6961 tgtaaaataa gcagttgagc agatttcttc tctaaaacca tctactgtaa aagattccga
     7021 cattccgtaa gatattgtaa atcaattact taaagataaa acgccccggc atttactctc
     7081 aggactcctc gttctgctcc agccagaata cccttttcga aacccgcgtt aagatgcttt
     7141 tcttaccgga aaagtcaggg ttcgcgacaa ataaacggcc tccccccaac cgtcgccagt
     7201 tcaatcctga atcgaagatc atgcagccca tcggggcggc aaaagttgcc gcaaacaacg
     7261 gtgttaatgc ccccaactgg ctcctggcag ggcggggcat gcttgatggc cctgaaaatc
     7321 acttttccct cacctgcccc gcgcatatta caggcggacg ctgaaaagcc atttcctctc
     7381 cgttgtgagg gatcgtatca ctgggtgaag ttggagatca ggaaggaacc gtcatcgcga
     7441 ttgagcgcga cttgggcgcg gccctgaaac agcagtatca gcgcccggcg tgcggccaaa
     7501 cattaatcgc ctgccagggt cggcttactc acccacataa gctggttaag ctcagaactc
     7561 ataaccgggc caaccgcgtc cattctgccc aatccgctcc ggccagtgaa cgcggctccg
     7621 gcttggcacg catgaaatcc gggatcgcgc gcaggcatgt ctgtccacct cagcgattat
     7681 gggcggaacg ggcgaacgga aatgcccccg ccccttcccg tcgacaccgg acttctggag
     7741 ccagccgtaa atgttggtcc cggctgtaaa gaaatcggct tggcccgagg ctaaaaattg
     7801 ctggtttctg gattgaatta agcgcgccct tccgtgacct taattgccgg ccatgcacct
     7861 cgcgaaaaac aggctccgca cgatcttaat cggcgtgatg ctgggcggta tcacgctggc
     7921 cgcgttctgg ccggtaatcc ataacgattt catcatctac gacgatgccg cctacgtgac
     7981 cgagaatccc caggtgctgg ccgggttgac gtggcggcag gcgggcttgg ccttccgaac
     8041 gagctgcgcc gggaactggc atccgctaac cgtgctctcc cacatgctgg acgtgcaact
     8101 tttcgggctg aacccggcag gccatcatct gaccagcctg ctgctgcacg ccgccaacgc
     8161 ggtgctgctg tttttagtgc tccgacgagc cacgcaaccc tccaaccccc aacttccaac
     8221 ctcccgcctc gaatctcgcc ccccaccggc cctcgaccct ttctggctct gcgcaatggt
     8281 ggccgggctc tttgccctgc acccattgcg cgtggaatcg gtcgcgtggg ttgccgagcg
     8341 aaaagacgtg ctcagcgctt tcttcttcct gttgacgctt tgggcctacg tgcggaatgc
     8401 ggagggccag accccggagt ccacaacccg cgccccactc tacgcaaccc gcttctacgt
     8461 tctcgcgctg gtcttcttcg cgcttgcgct catgagcaag gcaatggtgg tcacgctccc
     8521 gtttatcctg cttttgctcg attattggcc cttgcggcgg ctccagttct catccccttg
     8581 ctggctttcg actctccgtc gcttgctcct ggagaagctc cctttctttg cgctgacagc
     8641 cctcttcacc ggagtggcgt ttctcctgct gcggcaggcg ggcgccacgc aggagctgcc
     8701 gggcgttgga tgggggcacc gcctggcccg catctttgtc ggctacgaac agtatctcgc
     8761 caaaacgatc tggcccgttg accttgcgct tccctacccg cgcctggccg aagtgtccct
     8821 gggattggcg ctggccgggg cggccgcggt catcagcctg tcttttatag ccatttggca
     8881 cggacggcgg cgcctctatg cgccggtcgg ctggttttgg ttcctgggca tgctaatccc
     8941 ggtgatcggg cttgttggcg ttggcgcgca aaccatcgcg gaccgttata cctaccttcc
     9001 ttccataggg ctttccatcg tggccgcctg gggcctgggc gaaatcgccg gacgcccccg
     9061 gttgaggctt ccgatgattg gggcctccgt tctggcgctg gcggcgttgc tgctggccac
     9121 ccaccaccag ttgcgctatt ggcaaaacag cgaagcgctc ttccgtcacg ccctggcggt
     9181 caccgacgtc aatccgcttg cgcattccct gcttgcatcc gtcctggcgg aagccggaga
     9241 actggagaaa gccgaaaccc attgccgcga agcccggcgg ctcatgccga attgcgcgga
     9301 gacagagatt ctgtatgccc gggtgctggc tcgcagaaac aagttcgagg aagctatcgc
     9361 ggtcctctcg cagctcttgc ggcacagttc cgtggccccc gaagcccatt tcaacctggg
     9421 cgctatcttt ggcgagcaag gggctgcgtc ccaggccatg aacagctacc gccaagccct
     9481 ggcgttgaag ccggatttcc ccgaggcata cttcggcctc gccgccctct ttacccagca
     9541 gggtgacacc gccgccgcga ttgaggaata ctaccagggc ctgcgcaggc aaccggacgc
     9601 ccccgaggct ctcaacaacc ttgcctggat ccgtgccgcc agcgccaatg ccgccttccg
     9661 caacggcgaa gaagccgttc gcctcgcctc gcgtgcctgt gagctaagcg ggcggcaaaa
     9721 tcccgtcatg cttggcaccc tggccgctgc ctatgccgaa gccgggcgct ttgacgacgc
     9781 ggtcgcgacc gcgcaagagg cctgcaacat cgccttggcc aaaggacagg gcgacctagc
     9841 gaaaaagaac caggccctcc tggaactcta tcaatcccgc cgcccgtatc atgagtccct
     9901 cgaaccgttt ccggcctcgc cgtcgcacct ttagccctga agaaggctat tcgcatccga
     9961 tgcctggcca gggcttttct cgcgcctgac ttatccgacg acgtttcaac ctccaaggga
    10021 gcgatagata gcgcacggtt ggactaccca actgcaacgt ttcagacaag gcattcatga
    10081 agccagatgg catctggata ctcgttctcc cttgaaaaac tgcctcccgc caacttcata
    10141 ttcacaacca actgttttcc aatagtttac aacccatccc accgcgccat ctcgtcgttt
    10201 cggcccatcc cggcatccgc tgccattacc cagatgcctt ggacataccc tgcccccagg
    10261 tcgtgtacaa ggggtgtaca agggatgctc atcggaggtc catgggtttt tagctggtac
    10321 atccgatgag catcccttgt acaccccttg tacacggctt gaaggcaggg ggcgtctatg
    10381 gagtgccgaa gtcggcagga ttcggattcc tcctccattt tacccctctc cgcttgcgta
    10441 agccgttgat tttacaagcg aaaggcgttt agggttcagc agagatgccg ctgatttcgc
    10501 taaacagggg aacggcgggg ctatggt
//
LOCUS       DAOPQG010000120        10526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7184_length_10526_cov_17.133512, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000120 DAOPQG010000000
VERSION     DAOPQG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7184_length_10526_cov_17.133512"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKI07_11795"
     CDS             complement(<1..421)
                     /locus_tag="PKI07_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51766.1"
                     /translation="MKVLTSPRTGSLGAETYYQSPFGVCARRRTVPRDRPSERKAAAR
                     SYFGISSREWGLKLTEAQRERWNAAALHVPSRPWMGQYSHLSGQQFCVQINSTLRGLG
                     LAPVEEPPAPVVFGPNPVTGLEIVNDAQDGVRLRLNVG"
     gene            840..1853
                     /locus_tag="PKI07_11800"
     CDS             840..1853
                     /locus_tag="PKI07_11800"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOC51767.1"
                     /translation="MQAIQAEVVPSHTPALFRVAVRRAAEMLRAGELVALPTETVYGL
                     AANAGDAAAVARIFEVKGRPAHNPIIVHVASLEMAKRCVADWPVLADRLAAAFWPGPL
                     TLVLPRGKEIPGIVTAGGATVGIRWPSHPLIQAVIQECGFPLAAPSANPANRLSPTMA
                     EHVRKHLGGKIRLIIDGGRAQVGIESTVLDISTSPPRVLRPGLVHEQALLAVTRKLAT
                     GAGPNPEILRSPGLLPRHYSPQAKLEIWAWRDEEDLRARRARSGVRGSRVHLVAHTRI
                     PAGAGLGRVSVIPREAAAFARALYAELHECDAAGAELIVVEAPPEGPEWRAIADRLRR
                     AAG"
     gene            1914..3422
                     /locus_tag="PKI07_11805"
     CDS             1914..3422
                     /locus_tag="PKI07_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC51768.1"
                     /translation="MRRLVIAWGLAAASLAALAQAPVPAAPAVSLEDCLQRALEKNLD
                     LRIARYEPLRAQTDLQAAFAGYNPALTLGGGHQYSKSGGGFSPEIGAIPATTTDEDRF
                     ESGLSGLAPLGLQYRLQGNVSESRGKRFQPPLMLPFDSANGLASLSLAQPLLKNLWMD
                     QTRFHIRVARNRIQYTELGLQQAIMNTVTAVEKAYYDLVSAHENVAVQEKAVELAAQS
                     LKDTQRRREVGTVADLEVKQAEAQAASTQAALIAARSALAVQQNNLQQLITDDYAAAR
                     PMDLRPAAALGAPARVLDKQLSWGKGLRQRPDLLQARLDLERQGILLKYHYNQLFPEL
                     DLVGSYGHGAGGAGVSEFSQAFHQINRGNRPFYSYGAQLTFPLGNSGARAAYKKSKLE
                     KEQLLLALKRLEQDIMVAIDNDINRARSSYQQVAATRAAREYAQAALEAEQIKSQAGS
                     SILYLVLQMQRDLTAARGNEIQALAAYNKTLTQLSLDEGSTLERLGIHLELK"
     gene            3449..6112
                     /locus_tag="PKI07_11810"
     CDS             3449..6112
                     /locus_tag="PKI07_11810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC51769.1"
                     /translation="MTVAASASPRVSVEGKFFRLGPERFFVRGLAYGPFALPFAAPEQ
                     TRRDFAQIRDLGANLIRVYHVPPRWLLDLAAAHELKVLVDIPWNQHLCFLDSAARRAE
                     AMDAVRRAVSGCARHPAVFAFSVASELAPDIVRWSGPRAVSAFLERLMREAKRIDPDC
                     LCTFTSYPPTEALRPAAADFVCFNLYLHREKPFRDYLARLQIHADAKPLLLGEFGIDA
                     LREGEARQAEILAWQIESAFRGGLAGAVIFTFTDDWWRGGRLIEDWKMGLTTRDRQPR
                     PAAAGVRRQFRAAPYFPLHRSPRVSVVVASYNGARTLRACLASLARLNYPDYEVILVD
                     DGSTDDTPQIAAAHPQVRCFRHERNLGLSTARNTGIAAATGAIVAFTDADCRADPDWL
                     YYLVGSLLAGGFAGMGGPNLLPPEDSPVAAAVMASPGGPTQVMLTDRQAEHIPGCNMA
                     FYKWALDQIGGFDPTFRQAGDDVDLCWRLQQAGLRLGFSPAAVVWHYRRATVGAYLRQ
                     QLGYGEAEALLVRKHPECFNSFGGSLWRGRIYTSASPGSLLQPPIIYRGLFASAGFQF
                     LYAAEPAVNLLICTTPEYHVLVTLPLWVLSAIFHPLLPLALASLLLSLGVCLAAGARA
                     RIPAPRQRWWSRPLVSLLFGLQPIMRGWARYRGRLMQPPAPAAARPTLDSAALRHSEQ
                     SLREVHYWAERRIDRLAWAAEIVRRLDQQGWPNKPDVGWSDYDVEVYDARWSKLQLVT
                     VAEEYPRGKQLIRCRLRPRGAPRARIALGALGMAALFLCGLIGPKMPWIWLLLLALPL
                     FGGFLRREQRKLQSLIMAFLDDLAREWNLVKIPLPNGSPASGSPAKPPPLHSPGAAAP
                     TPFSPPVNDNTAGPATPRPTTPASIPPRDPA"
     gene            6518..7840
                     /locus_tag="PKI07_11815"
     CDS             6518..7840
                     /locus_tag="PKI07_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC51770.1"
                     /translation="MSRQNKAPLLEENRATPPEPLPLPHPAAARPAGSRLTPPKSPAA
                     PPPCVLLEKARNFTAAADLRTAGLYPYFRRISSAQDTEVIIEGRKVLMLGSNSYLGLT
                     NHPRIKEAARAAVDKYGSGCAGSRFLNGTLDIHLELESALARLVNKEAVLLYSTGFQV
                     NLGVISALVAKGEYVLGDKSNHASIVEGCLLAQGKFLRFAHRDMAALESRMDKLEPEA
                     GKLIVVDGVFSMEGDIIQLPEVCRIASQYGAAVMVDDAHAIGVLGPNGAGTASHFGLT
                     DQVHLIMGTFSKSLASLGGFIGADAATIDYLKHHSRALIFSASMSPANAAAVLAALQI
                     MEQEPERIAQLWRNTRRMKQGLRELGFNLGDSQTPILPVYCGGLMRAFQMCKRLQEEG
                     VFVNPVVSPAVAPGQELIRISLMATHTDQQVDFALDKLGKVGKELHLL"
     gene            7841..8821
                     /locus_tag="PKI07_11820"
     CDS             7841..8821
                     /locus_tag="PKI07_11820"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC51771.1"
                     /translation="MKILLTGASGFVGSHILDCLRQRGLPTVLLLRPSSNRRFLASHL
                     AEVEVRSGAVDDPASLRRAMDGITHVIHCAGATKAVRTSGFYEVNQGGTRHVVAAINE
                     RPGQVRRLVHISSLAAAGPAQPARPAREEDAPRPVSEYGKSKLAAETEVRDHCRAESV
                     ILRPPAVYGPRDAEFLRLFRAVKRHVRPGLSGAQALSLVFVKDLAAAVVACLTHPAAA
                     GRTYYVAAREITTARALAETVAAQMNRWTLPLPLPVALLWPICLGQEAWSRLTGRASV
                     LSLQKFAELRAPGWVCDPGRLERETGCVCATTLEAGLAETRRWYQEQGWL"
     gene            8818..9783
                     /locus_tag="PKI07_11825"
     CDS             8818..9783
                     /locus_tag="PKI07_11825"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOC51772.1"
                     /translation="MKHYTFVDYATQAYLALVGLLILLFHNHTVPAWPRLVAAHAAAL
                     VLIHGLIRLHGRGRPGWLLDFLRHFYPVLLYTAFFIETGMLNRMFFPDYLDPLVARWE
                     QWLFGCQPSVLFMEALPWLAVSELFYAAYFSYYIMIAGVGLALFLRHRRQFFHYVSVV
                     SFLFYVCYLIYIILPVIGSRAFFRQVDGYALPEPIQQLAVTDEYPAAVQAGVFFQIMK
                     WIYREFESPGSAFPSSHVAVALCTVFFSFRYLRRIRYAHLGVAVLLCLSTVYCRYHYV
                     VDVLAGLVTAAVVIPLGNWLYFRFSRPAPTDATPASGPSPIAQKP"
     gene            10120..10503
                     /locus_tag="PKI07_11830"
     CDS             10120..10503
                     /locus_tag="PKI07_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOC51773.1"
                     /translation="MPIETHLSRRERQIMDVLHRHGRATAAEVQAALPAAPGYSAVRA
                     LLRILEKKGHVRHHRAGASYVYLPRASRATARRSALQRVVATFFQGSVTQAVAALLET
                     ADTRLTDAELNKLRQMINQARKEGR"
BASE COUNT         1601 a         3481 c         3610 g         1834 t
ORIGIN      
        1 ccccgacgtt gagccgcagc cgaactccgt cttgggcgtc attgacgatc tccagcccgg
       61 tgaccgggtt ggggccgaag accaccgggg cgggcggttc ctccaccggc gccagaccca
      121 gcccccgcag ggtgctgttg atctggacgc aaaactgctg cccacttaag tgcgaatact
      181 gccccatcca gggccggctg ggaacgtgca gcgccgcggc gttccagcgt tcgcgctgcg
      241 cttcggtgag cttgagtccc cactcccgcg aggagatccc gaagtacgac cgtgcggccg
      301 ccttgcgctc cgagggtcgg tctcggggaa cggtgcgccg ccgggcgcac acgccgaagg
      361 gggattggta ataggtttcc gcgcccagac tgcccgtgcg cggagatgtg agtactttca
      421 taatgtctgt cctctctaat ggttgtttta attgctgttc tattacggcc cggaggcgat
      481 tcggcttccc gccactcctt gggctggaag tccgccccga ggtaatacgg gaaggctggc
      541 gggcggaggg gcttcgcgca agggcaattt ttcaggattg gtggtaacag gccggtgcgg
      601 agcgccgcgt gggggcacgc ggcctacagg gggcgggagg gtgcgcgggg gcctgtgggt
      661 tgccgggggc gcggtgctgc gagagggcat gggggctgcc gcggcgtgct tctatttcct
      721 gcaggccgcg tgtgctcacg cggcgctcgg gggccgtcct ggccctgggg cggaacgcgg
      781 gcggcggttt ttttgtatgc gcggggcggg tttatgggca aattggcggg gtggcaaaga
      841 tgcaggcgat tcaggctgaa gttgtgccgt cgcacacccc cgcattgttc cgggtggcgg
      901 tgcggcgggc ggcggagatg ctgcgggcgg gcgagttggt cgcgctgccc accgaaaccg
      961 tttacggcct ggcggccaat gccggggacg cggcggcggt ggcccgcatt tttgaggtca
     1021 aagggcgccc ggcgcacaac cccattattg tgcatgtcgc cagcctcgag atggcgaagc
     1081 gttgcgtggc ggactggccg gttctggcgg accggctggc ggcggcattc tggccggggc
     1141 cgctgacgct ggtgttgccg cgggggaaag aaatccccgg cattgtcacg gcgggggggg
     1201 cgaccgtcgg gatccgctgg ccgagccatc cgttgatcca agcggtcatc caggaatgcg
     1261 gttttccgct cgcggcgccg agcgccaatc ccgccaatcg cctctcgccg accatggccg
     1321 agcatgtgcg gaaacatctc ggcgggaaga ttcgcctgat tattgacggc ggccgggccc
     1381 aggtcggcat tgaatccacg gtcctggata tttccacttc tccgccccgc gtgctgcgcc
     1441 cgggcctggt ccatgagcag gcgctgctgg ccgtcacccg gaaactggcg acgggcgccg
     1501 ggccgaaccc ggaaatcctc cggagcccgg gcttgctgcc ccggcactat tcgccccagg
     1561 cgaaactgga gatttgggcg tggcgggatg aggaggattt gcgggcccgc cgcgcgcgca
     1621 gcggcgtgcg cggctcccgc gtgcacctgg tggcgcatac ccggatcccg gcgggggcgg
     1681 ggctggggcg ggtgtcggtg attccccgcg aggcggcggc gtttgcgcgg gccctctacg
     1741 ccgagctgca tgaatgtgac gccgcggggg cggagctgat tgtggtggag gcgccgcccg
     1801 aggggcccga gtggcgcgcc atcgccgacc ggttgcgccg ggcggcgggc tgaggcggcc
     1861 gggcgcgagg tttatttgac tcccggcggc ggattccgca agctgcaatt cccatgagac
     1921 gcttggtaat agcctggggg ctggccgccg ccagcctggc cgccctggcg caggccccgg
     1981 tgcccgccgc gccggcggtc tccctggagg actgcctcca gcgcgccctg gagaagaatc
     2041 tcgatttgcg cattgcgcgc tatgagccgc tgcgggcgca aacggatttg caggccgcct
     2101 tcgccgggta taaccccgct ttgacactcg gcggggggca ccagtacagc aaatccggcg
     2161 ggggattcag cccggagatc ggcgcgattc ccgccaccac gaccgacgaa gaccgcttcg
     2221 agagcggcct gagcgggctg gcgcccctgg ggctgcagta tcgcttgcag ggaaatgttt
     2281 ccgagagccg cggcaaacgc tttcaaccgc ccctcatgct gcccttcgac agcgccaacg
     2341 gcctggcctc cctgagcctg gcccagccgc tgttgaaaaa tctctggatg gaccagacac
     2401 gcttccacat ccgggtggcg cggaaccgca tccaatatac ggaattgggc ttgcagcagg
     2461 cgatcatgaa caccgtgacg gcggtggaaa aagcctacta cgacctggtt tccgcgcacg
     2521 agaacgtcgc ggtgcaggag aaggccgtcg agctggccgc ccaatcgctc aaagacaccc
     2581 agcggcggcg cgaggtgggc acggtggcgg acctggaggt caagcaagcc gaggcccagg
     2641 ccgcctcgac gcaagcggcg ctcatcgccg cccgcagcgc gctggccgtg cagcaaaaca
     2701 acctgcaaca actgatcacc gacgactacg ccgccgcccg gcccatggac ctgcggcccg
     2761 ccgccgcgct gggcgcgccg gcgcgggtgt tggacaagca gctcagttgg ggcaaagggc
     2821 tgcggcagcg cccggatttg ctccaggccc ggctcgatct cgagcgccag ggaatcctcc
     2881 tgaaatacca ctacaaccag ctatttccgg agctggattt ggtgggcagc tacggccacg
     2941 gcgccggcgg ggcgggcgtc tccgagttca gccaggcgtt ccaccaaatc aaccgcggca
     3001 accggccctt ttactcctat ggcgcgcaac tgacgtttcc gctgggcaac tccggcgccc
     3061 gcgccgccta caagaagagc aaactggaaa aggagcaact gctgctggcc ctcaaacggc
     3121 tggaacagga catcatggtg gcgattgaca acgacattaa ccgggcccgg tccagctacc
     3181 agcaggtggc cgccacccgc gccgcccgcg aatacgcgca ggccgcgctg gaagccgagc
     3241 agatcaaaag ccaggccggc tccagcatcc tctacctcgt cctgcaaatg cagcgtgacc
     3301 tgaccgccgc gcgcggcaac gaaatccagg ccctggccgc ctacaacaag accctgaccc
     3361 agctcagcct tgacgaaggc tccaccctcg agcggctggg catccacctc gaattgaagt
     3421 aggggtccgg cgccggcgtc taaaccgcat gacagtcgcg gcttctgcat cgccccgggt
     3481 cagcgtggag ggcaaatttt tccggctcgg cccggagcgc ttctttgttc ggggcctggc
     3541 ctacggcccc ttcgcgctgc cgttcgccgc gccggagcaa acccgccggg actttgcgca
     3601 aatccgcgac ctgggggcca atctcatccg ggtttaccac gtcccgcccc ggtggctcct
     3661 cgacctggcc gcggcccacg agctcaaggt gctggtggat attccgtgga accagcatct
     3721 gtgtttcctg gactccgccg cccggcgcgc cgaagccatg gacgccgtgc gccgcgccgt
     3781 gagcggctgc gcgcgccacc cggcggtctt cgcgttcagc gtcgcctccg agctggcccc
     3841 ggacatcgtg cgctggagcg gcccccgggc cgtctccgct tttctggagc ggctgatgcg
     3901 cgaagccaag cggattgacc ccgactgtct ctgcaccttc accagctacc cccccaccga
     3961 ggcgctgcgc ccggcggccg cggactttgt ctgctttaac ctctacctgc accgggagaa
     4021 acccttccgg gattacctgg cccggctgca aatacacgcc gacgccaaac ccctgctgct
     4081 gggcgagttt ggcattgacg cgctgcgcga gggcgaggcg cgccaggcgg aaatactggc
     4141 gtggcaaatc gagagcgcct tccggggagg gctggccggc gcggtgatct ttaccttcac
     4201 cgacgactgg tggcgcgggg ggcgactgat cgaagactgg aaaatggggc tgaccacgcg
     4261 cgaccggcag ccccggcccg ccgcggccgg cgtgcggcgg cagttccgcg ccgcgccgta
     4321 tttccccctg caccgctccc cccgcgtctc ggtggtggtg gccagctaca acggggcgcg
     4381 cacgttgcgg gcctgcctgg cttcgctggc gcggctcaat tacccggact acgaagtcat
     4441 cctcgtggac gacggctcga ccgacgacac cccccaaatc gccgccgccc atccccaggt
     4501 ccgctgcttc cgccacgagc ggaacctcgg cctctcgacc gcgcgcaaca ccggcatcgc
     4561 cgccgcgacg ggcgcgatcg tggcgttcac cgacgccgat tgccgggcgg accccgattg
     4621 gctctactac ctcgtcggca gcctgctggc cggcggcttc gcgggcatgg gcggccccaa
     4681 tctcctgccg ccggaagatt cgcccgtcgc cgccgccgtg atggcctcgc ccggcgggcc
     4741 gacgcaagtg atgctgacgg atcgccaggc ggagcacatc cccggctgca acatggcctt
     4801 ttataaatgg gcgctggacc agatcggggg ctttgacccc accttccgcc aggcgggcga
     4861 tgatgtggac ttatgctggc gcctgcaaca ggcggggctg cggctcggct tcagcccggc
     4921 ggcggtggtc tggcattacc ggcgcgcgac cgttggcgcc tacttgaggc agcagctcgg
     4981 ctacggcgag gcggaagcgc tgctggtgcg caagcatccc gaatgtttca actccttcgg
     5041 cggcagcctt tggcgcgggc gcatttacac cagcgccagc ccgggcagcc tgctgcagcc
     5101 cccgatcatc taccggggcc tcttcgccag cgcgggcttc caattcctct acgccgccga
     5161 gccggccgtc aatctcctga tctgcaccac gcccgaatac catgtcctgg tgaccctgcc
     5221 gttgtgggtg ttgtcggcga tctttcatcc cctgctgccg ctggccctcg ccagcctgct
     5281 gctctcgctg ggcgtctgcc tggcggcggg cgcgcgcgcc cggatccccg ccccgcggca
     5341 acgctggtgg tcccgcccgc tcgtcagcct gcttttcggc ctgcagccca tcatgcgcgg
     5401 ctgggcccgc taccgcgggc gcctgatgca accgcccgcc cccgccgccg cgcggccgac
     5461 gctcgattcc gccgcgctgc gccacagcga gcaatcgctg cgcgaagtcc actactgggc
     5521 ggagcggcgc attgatcggc tggcctgggc cgccgaaatc gtgcgccgcc tggatcagca
     5581 gggctggccc aataaacccg atgtcggctg gagcgactat gacgtcgaag tctatgacgc
     5641 gcgctggagc aaactgcagt tggtcaccgt ggcggaagaa tatccccgcg gcaaacagct
     5701 catccgctgc cgcctgcgcc cgcgcggggc gccgcgcgcc cggatcgccc tgggggcgct
     5761 gggcatggcg gcccttttcc tgtgcgggct gatcgggccg aaaatgccct ggatttggct
     5821 gctgctgctg gcgctgccgc tgtttggcgg gttcctccgg cgcgagcagc ggaaattgca
     5881 gagcctcatc atggcgttcc tggatgacct ggccagggaa tggaacctgg tcaagattcc
     5941 cctccccaac ggctcgccgg cctcgggttc acccgccaaa ccacccccgc tccattcgcc
     6001 cggcgcggcc gcgcccacgc ccttttctcc gcccgttaac gataataccg cgggcccagc
     6061 cactccccgc ccgacgaccc ccgcgagcat cccgccccga gaccccgcct gagcggaaag
     6121 gaagtcggct gggttggcgg cctgaactcc ctccattggg gagccaaagc gctccggcag
     6181 cggcacgggg aaggcgggtg acggccgggg cgggatgccg ttttcatggg gttttagctg
     6241 gcaggccggg ggttggcacc gggaacgcca atcccccgga gtgggggccc ggcgcggcgg
     6301 cgacgccggg gaggccaact ttatccttgc tctccgggtg ataaacgata aaaggaagcc
     6361 ttgagaacga ttgaataggt tggttgagtg gtggtccagt ttttggcact atgagtagaa
     6421 gcggtgagtt tatatctatc ggcaggcaac gggccaggca atccgggcgg ccgatgaatg
     6481 tcagggcgga aggcgcggcg gcgcgtccag ccgatttatg agccgccaga acaaagcgcc
     6541 tttgctggag gaaaaccggg ccaccccgcc ggagcctctc cccttgcccc acccggccgc
     6601 ggcccggccg gcgggcagcc ggttgacgcc gccgaaatcg cccgccgccc cgcccccgtg
     6661 cgttctgctg gagaaagccc ggaactttac ggcggcggcg gatttgcgga ccgcggggtt
     6721 gtatccctat tttcggcgga tttcatcggc ccaggacacc gaggttatca tcgagggccg
     6781 gaaggttctg atgctggggt ccaacagcta cctgggcctg accaatcacc cccggattaa
     6841 agaggcggcg cgggcggcgg tggacaagta tgggtcgggg tgcgcgggtt cgcgctttct
     6901 gaatggcacg ctggatatcc acctggagct ggagtccgcg ctggcccggc tggtcaacaa
     6961 ggaggcggtg ctgctttaca gcaccggctt ccaggtcaac ctgggcgtca tcagcgcgct
     7021 ggtggccaag ggcgagtatg tgctgggcga taaaagcaat cacgccagca ttgtcgaggg
     7081 atgcctgctg gcccagggca aattcctgcg ctttgcgcac cgggacatgg ccgccctgga
     7141 aagccggatg gacaagctcg agcccgaggc gggaaagctc atcgtcgtgg acggggtgtt
     7201 cagcatggag ggcgacatca tccaactgcc ggaagtgtgc cgcatagcca gccagtatgg
     7261 cgcggcggtc atggtggatg acgcccacgc gatcggcgtt ttaggcccga acggcgccgg
     7321 cacggccagc catttcgggc tgaccgacca ggtgcatctg atcatgggca cgttcagcaa
     7381 atcgctggcc agcctgggcg ggttcattgg ggcggacgcg gctacgattg attatctgaa
     7441 gcaccattcg cgggcgttga tcttcagcgc gagcatgagc ccggccaacg ccgcggcggt
     7501 gctggccgcg ctgcaaatca tggagcagga accggagcgc atcgcgcagc tttggcgcaa
     7561 tacgcgccgc atgaagcagg ggctgcggga actgggcttc aacctgggcg attcgcagac
     7621 gccgattttg ccggtgtatt gcggcggttt gatgcgcgcc ttccagatgt gcaagcggct
     7681 gcaggaggag ggcgtgtttg tcaacccggt ggtgtctccg gcggtggcgc ccgggcagga
     7741 attgatccgc atcagcctga tggcgacgca caccgaccag caggtggatt tcgccctcga
     7801 caagctcgga aaggtgggca aggaactgca cctgctctga ttgaagattc tgctcacagg
     7861 agcaagcggt ttcgttggca gccatattct cgattgcctc cgccagcggg ggctgcccac
     7921 cgtcctgttg ctccggccgt ccagcaaccg gcggttcctc gcgtcccacc tggcggaggt
     7981 cgaggtgcgt tccggcgcgg tggatgaccc cgccagcctg cggcgggcga tggatggcat
     8041 cacgcatgta attcattgcg cgggcgccac caaagccgtc cggacctccg gcttttacga
     8101 ggtgaaccag ggcggcacgc gccatgtcgt ggcggccatc aacgaacggc cgggccaggt
     8161 gcggcggctg gtgcatattt cgagcctggc ggcggcgggg cccgcgcaac cggcgcggcc
     8221 ggcgcgcgag gaggatgccc cgcggcccgt gtccgaatac ggcaagagca agctggcggc
     8281 ggaaacggaa gtccgggacc actgccgcgc cgagtcggtc attctgcgcc ccccggcggt
     8341 gtatggcccg cgcgatgcgg agttcctgcg gctgttccgg gcggtgaagc gccacgtgcg
     8401 gcccgggttg agcggcgcgc aggcgctcag cctggtgttt gtgaaggacc tggccgccgc
     8461 cgtcgtggcc tgcctgacgc atccggcggc cgcgggccga acctactatg tggccgcgcg
     8521 ggaaatcacc acggcgcgcg cgctggcgga gaccgtcgcc gcccaaatga accgctggac
     8581 gttgcccctg ccgctgcccg tggcgctgct gtggccgatt tgcctggggc aggaagcctg
     8641 gtcgcgcctg acgggccggg ccagcgtttt gagtttgcag aagtttgccg aactgcgcgc
     8701 gccgggctgg gtgtgcgacc cgggccggct ggagcgcgag accgggtgcg tctgcgcgac
     8761 gacgctggag gcgggcctgg cggaaacgcg gcgctggtat caggagcagg gctggctgtg
     8821 aagcactaca cctttgtgga ctacgcgacg caggcctacc tggcgctggt ggggttgttg
     8881 atcctgcttt tccacaatca caccgtgccg gcctggccgc ggctggtggc cgcgcacgcg
     8941 gcggccctgg tgctgatcca cgggcttatc cgcctgcacg ggcggggccg gccgggctgg
     9001 ctgctggatt ttctccggca tttttacccg gtgctgctct atacggcttt ctttatcgag
     9061 acgggtatgc tgaaccgcat gtttttcccg gattacctgg atcccctggt ggcgcgttgg
     9121 gagcagtggc tctttggctg ccagcccagc gttcttttta tggaagcgct gccttggctg
     9181 gccgtcagcg agttgttcta cgcggcttac ttctcctact acatcatgat cgcgggcgtg
     9241 gggctcgccc tgttcctgcg ccaccggcgg cagttttttc actacgtctc cgtggtgtcg
     9301 tttctgttct atgtctgcta cctgatctac atcatcctgc cggtcatcgg gtcgcgggcg
     9361 ttcttccgcc aggtggatgg ctatgcgctg ccggagccca tccagcagtt ggcggtgacg
     9421 gatgaatacc ccgcggccgt gcaggcgggc gtcttttttc aaatcatgaa gtggatttac
     9481 cgcgagttcg agtcgccggg ctcggcgttt cccagcagcc atgtcgcggt ggccttatgc
     9541 accgtttttt tctctttccg ctacctgcgc cgcatccgct atgcgcacct gggggtggcg
     9601 gtgctgcttt gcctttcgac ggtgtattgc cgctaccatt acgtggtgga tgtgctggcg
     9661 ggcctggtga ccgcggcggt ggtcattccc ctcggcaact ggctctattt ccgctttagc
     9721 cgccccgcgc cgaccgacgc aaccccggcc tccggcccgt ctccaatcgc gcaaaaaccg
     9781 tgaagcccgt tccctcccgg ggacaaggtc gcgcccgggg caaggaaccg gcgggaggat
     9841 tgttttcccc tggaacaaag cacgagagga agaacggtct gcgcgcatag ccgctttcct
     9901 ctcctccggt tgctgcccgg ctttgctccg cagccgccgc caatcattcc cccgacgccg
     9961 cgatccggcc gccggactcc ggcgttgcgg cgccgccgcc ggggcgggcc ttcgcggggg
    10021 ccggcttcgg ctcccggcgg ggggagccgg ggtgttaaaa ttttagcact ttcgacttga
    10081 ctgccttcgg ggaagtgtta aatttttagc atatttgtca tgcctatcga aacgcatctc
    10141 agccgccgcg agcgccagat catggatgtt ttgcatcgcc acgggcgcgc caccgccgcc
    10201 gaagtgcagg ccgccctgcc cgccgccccc ggctattcgg ccgtccgcgc actgctgcgc
    10261 attttggaaa agaagggaca cgtccggcat caccgcgcgg gcgccagcta tgtgtatttg
    10321 ccgcgcgcct cgcgggcgac cgcacggcgt tcggcgttgc agcgggtggt cgcgacgttc
    10381 tttcaggggt cggtgacgca ggcggtggca gcgctgctgg aaacggcgga cacccggctg
    10441 acggatgcgg agttgaacaa attgcgccaa atgataaacc aggctcgcaa ggagggccgt
    10501 tgatatgaac ccattgcttg aaacta
//
LOCUS       DAOPQG010000121        10416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7287_length_10416_cov_14.181546, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000121 DAOPQG010000000
VERSION     DAOPQG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7287_length_10416_cov_14.181546"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..955
                     /locus_tag="PKI07_11835"
     CDS             449..955
                     /locus_tag="PKI07_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51774.1"
                     /translation="MSLDAAQRTKVAEWIEQGLKLSEIQSRLASEMGVRLTYMEARLL
                     VDDLKLTPKDAEPSEVVESALVDATRGAPPEAAQARGNPQSAAPESALTGGSSGNVAV
                     SVDQVARPGAMVSGKVTFSDGNTAAWQFDELGRLALAPSQAGYRPSPADVEKFQMALD
                     AELNKLGL"
     gene            981..2054
                     /locus_tag="PKI07_11840"
     CDS             981..2054
                     /locus_tag="PKI07_11840"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOC51775.1"
                     /translation="MSKPDLTHLTRRRFLQVAAYGLALGNAGLAAAAGRRTTAPFFRT
                     RGVVLTPDDLTLPDWPKRARMAGLTTLALHAPHSPRRLADYMRSAPGRKFLAACRRHR
                     LAVEYELHAMQDLLPREYFDRAPKMFRMDEKGQRVRDGNLCVHSAPGMQIVADNAVAL
                     AQALRPTTGRYFYWGDDGCTWCRCPQCNGLSDSDQALLVANRILEALRKVDARAQVAH
                     LAYHNTLPPPRKVKPEPGIFLEYAPIHRRYDAPFEQSDDAQTRREFELLDANLAVFDR
                     TTAQVLEYWLDASLFSKWKKPAVKVPFLPQVLAADLNTYGRRGIRHVTSFGVYLDAEY
                     VSRHGEPPLQEYGEQLRRWAPDK"
     gene            2260..3015
                     /locus_tag="PKI07_11845"
     CDS             2260..3015
                     /locus_tag="PKI07_11845"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOC51776.1"
                     /translation="MQAAKLVELLGESSQILFFTGAGISTGSGIPDFRGPDGVWKYRQ
                     PVYYHDFMRSEAARIEHWDFKLEGWEAFRTARPNAVHEAIVRLERAGKVRAVVTQNID
                     GLHARAGTAAGLLIELHGTNRLIECQTCGRRSEPEAHFEYFRKTRRPPVCECGGFLKP
                     ATISFGQNLRIEDLEKAEDMAQGADLVVALGSTLSVHPAADIPLLAARRGAPYVIINR
                     GPTEHDEMPEVTLRIEGDVGEIFPPAVAAALKV"
     gene            3151..4005
                     /gene="nadC"
                     /locus_tag="PKI07_11850"
     CDS             3151..4005
                     /gene="nadC"
                     /locus_tag="PKI07_11850"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOC51777.1"
                     /translation="MKPLSVEKVRTVVRSALAEDLGRGDVTTLATVPESAHAWAEIRP
                     REAIVVAGLSLAEAAFREISPAVRVKRATADGRRAAAGEPLLVINGPARALLSAERVA
                     LNFLQRLCGIATLTAQFVAAVRGTRAKILDTRKTTPGWRWLEKYAVACGGGRNHRFGL
                     FDMVLIKDNHLAVLKDKTPNAIVAAVRRARARYPKLKVEVEADTLEQVAQAVAAGADL
                     VLLDNMSLPQLRRAVAQCKGRALTEASGGVTLRRVRALAQTGVDYISVGALTHSARAV
                     DIGLDFRS"
     gene            4031..4999
                     /locus_tag="PKI07_11855"
     CDS             4031..4999
                     /locus_tag="PKI07_11855"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOC51778.1"
                     /translation="MQILSALRGPGDGAVSGAELSQKLGVSRAAVWAHIEDLRRLGYD
                     IEASPHRGYRLLSAPDLLHADDMRSRLGPTRVIGRDIRVFEETASTSDVIEKLARDGV
                     REGVVVFAESQTRGRGRLGRKWVSPARRGLWFSVLLRPAVRPQEVTRLTVASATAVRR
                     AIESLTGLKPKIKWPNDILIRGRKVAGILTELSAELDCVRHVALGIGVNVNLEPGDLP
                     PELRAQTTSLKIESSRTVLRPELAVAILRELDRDYARTATGQFAALAEEWEEHSATMG
                     QEIVIRVGDRQVRGRAESLDDDGALLLRSDHGRLERVVGGDVALAK"
     gene            4996..5817
                     /locus_tag="PKI07_11860"
     CDS             4996..5817
                     /locus_tag="PKI07_11860"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOC51779.1"
                     /translation="MILLFDIGNTHTHLGLADARRVLKQANLPTVAWGRGSPRGVLQR
                     FAGGARLEGAVLCSVVPRATAWVHQAIRQLWNLPCLELTSKTLCGVGINYPRPATIGA
                     DRLANAVAARHHFGAPVVVVDFGTAVTFDVVDRAGDYVGGIIAPGLSAMTDYLHEKTA
                     LLPRIRIREVQSVIGKSTEQAMRVGAVQGYRGLIQELIRALKRELKAPRLPVVATGGC
                     AELIGARLKVITAVDPLLTLEGLRLEWQAKHPDRGDKSMSERGRAQRSQETPAIC"
     gene            5828..6766
                     /gene="rarD"
                     /locus_tag="PKI07_11865"
     CDS             5828..6766
                     /gene="rarD"
                     /locus_tag="PKI07_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOC51780.1"
                     /translation="MIQKADNHSALGNAMPSVSRWHSRAGLSYGIGAYMVWGVIPLYF
                     HVVAEVPPWEILCHRIAWSALFLALVVSVRGEWQALRPILRSRRKMLLLSVGSVLIAL
                     NWLIFIYAVISQQVLEASLGYFINPLLSVALGMIFLRERLQGWQWFAVGIAGVAVANL
                     AWRGSGFPWIAVSLAGTFGFYGLVRKAVNVNSLHGLLVETALLVPLGLLVLAIVPAPP
                     LPVLTLGVLSLSGIITAVPLLMFGAALRRLKLSTIGFLQYIGPTLQLLVATAIFREPL
                     DRIKLASFILCWLGIAVYTADSVLSNSLQPVADEPE"
     gene            complement(6873..8135)
                     /locus_tag="PKI07_11870"
     CDS             complement(6873..8135)
                     /locus_tag="PKI07_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC51781.1"
                     /translation="MAAKSRFLDKVLPRIGRLDEAGLQTVLQRLARERSFLETLFNTI
                     EDGVLVVDKSGRIIYFNQAVTRLLGLQPKAENQNITSFIPDLEWGKIRHFDLADGSGV
                     VRHEFEIHYPRPRFLRLYAAPLAGEAAGGTGVALILHDATEARQKTIEVIESERVQAL
                     TLLAASVAHEIGNPLNALHIHLQLMERELKKLRSAALSQPLASASSRLRLRARSRDAS
                     DDNIARDLVPKLEQYLSVARGEINRLDYIITQFLQAIRPSQPQLKPASLNEVVQKTLQ
                     LLQVEMDNRGLQLRTRLAPQLPLAPLDATQIQQLLVNLIKNAIQAMTRGGTLTVQTGE
                     GADGVWVSVADTGSGIQQEQVNRIFEPFYTTKEKGTGLGLMIVQRIVRAHGGHLELES
                     REDRGTTFRIWLPLHERKPRLLEAPSPD"
     gene            complement(8272..9270)
                     /gene="corA"
                     /locus_tag="PKI07_11875"
     CDS             complement(8272..9270)
                     /gene="corA"
                     /locus_tag="PKI07_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOC51782.1"
                     /translation="MIRSLVFTTQGRLHSKDIEMFLMPTLLSDTNLFLWVDLESPTAE
                     ETKYVLEDVFHFHPLSIEDCVTASPSPKVEEYSPKEGDWFAPYLFLVIHAVDYSRKDG
                     VFATSELSLFLGKNFLVTYHDGPLRSVSQTEERAVKAITNIARAPDRVAHTLLDSIVD
                     NYKPALDELSLEIAELEQQALQDPSHHILNKIIQVKREVLHLRQIIGPQREVLARFAR
                     GEFKLIRAHLVPYYRDIYDTLFHISELAQGYADTLTSILHVFLNMSSNRTSEVVKLLT
                     MITVITTPMMLVGTWYGMNFKNMPEVDWTQGYLAAFAVTLLSTLAIWWYFRKKKWF"
BASE COUNT         1970 a         2805 c         3393 g         2248 t
ORIGIN      
        1 ctgttcttgc catgcctctc aatacaatgc aactactaac aggaaatggt attagctggt
       61 gcatccgatg ggcatccctt gtacacccct tgtacacggc gtagaggcag ggtaaatcca
      121 agtgatgtcc aaggcaggct tcaaccgaaa cgagttgggt tccgattcta tttcactctt
      181 cccaatgtgc gtaagtcatt gatcttacag gcaaaagacg tttgggattc agcagctatg
      241 ccgctgattt cgctaaacaa gggatcgttg gggttatgac cagggcgctc gggacctccc
      301 gggctccgat agaacgcggg cgggcggttg aaccacctct ctaaatcgtt tcggtttagc
      361 cactcaggca ggaagcgtgg agggcgcgca aggcaaaaca aaggttgaat tagcgggcgt
      421 tcgcggtgaa taatgggcta tacggattat gagcctggat gctgcacaac ggactaaagt
      481 ggcggagtgg atcgagcagg gcctgaagct ttctgagatt cagagccggc tggcttcgga
      541 aatgggggtt cggctgacct atatggaggc gcgcctgttg gtggatgacc tgaaacttac
      601 gccgaaagat gccgaaccat ctgaagtggt tgagtccgcg cttgttgacg ccacgcgcgg
      661 ggcaccaccg gaggcggccc aagccagggg caacccacaa tccgccgcgc ccgagtcggc
      721 actgacgggc ggcagttctg gaaacgtggc ggtctcggtg gatcaggttg cccgacccgg
      781 cgcgatggta agcggcaaag tcacttttag cgatggcaac accgcggcct ggcaatttga
      841 tgagttgggg cggctggctc tggcgccgtc ccaagccggc tatcggccct cgccggccga
      901 tgtggagaag ttccagatgg cactggatgc tgaactgaac aaactggggc tttgaaccgc
      961 ggaagtattc gcaccctgga atgagtaaac ctgatttaac gcacctgacc cgccgacggt
     1021 ttttgcaagt cgcggcgtat ggattggccc tgggcaatgc cggccttgcc gccgccgccg
     1081 ggcggcgcac cacggcgcct ttcttccgca cgcgcggcgt ggtgctgacc cccgacgacc
     1141 tgacgcttcc cgattggccc aaacgcgccc ggatggcggg gttgaccact cttgcgctcc
     1201 acgcgcccca ttcgccgcgc cgactggctg actatatgcg gtcagcgccc gggcggaaat
     1261 tcctggctgc ttgccggcgg catcgcctgg cggttgaata cgaattgcac gccatgcagg
     1321 atctgctgcc gcgggagtat tttgaccggg cccccaaaat gttccgtatg gacgaaaaag
     1381 gccagcgggt tcgcgatggg aatttgtgcg tgcactcggc cccggggatg cagattgttg
     1441 ccgataatgc ggttgccctg gcgcaggcgc tgcgccccac cacgggccgt tatttttact
     1501 ggggcgacga tggttgtact tggtgccgct gcccgcaatg caacggcctg agcgacagtg
     1561 accaggcatt gcttgtcgcc aatcgaatac tcgaagccct ccgcaaggtg gatgcccggg
     1621 cgcaggttgc ccacctggcc taccataaca cgctgccacc gccccggaag gtcaagcccg
     1681 agccgggcat tttccttgaa tacgcgccga tccaccgccg ttatgacgcg ccgtttgagc
     1741 agtccgacga tgcccagacg cggcgggaat ttgagttgct cgatgcgaac ctggcggttt
     1801 ttgaccgaac gacagcgcag gtgttggaat actggcttga tgcttcgctt ttttccaagt
     1861 ggaagaaacc cgccgtgaag gtgccgttcc ttccgcaggt cctggcagcg gatttgaata
     1921 cctacggtcg ccgtggcatt cgccatgtga caagctttgg ggtgtacttg gatgcggaat
     1981 acgtttcccg gcatggggaa cccccgctcc aagagtatgg cgagcaactt cgccggtggg
     2041 cgcccgacaa atgagacggc cgttctaaac gaggaacaga gcgcctcggg tgcgaagctg
     2101 cgggcgtggc tggcggcagg acgccgccgg cagcaagcgg gttgcgtgcc ctcatcgctt
     2161 cctgcggcag ggccaagggc ggccgggtga ttccggcgcg aggttcgttg ccggtttttg
     2221 tttggagcgg tcgcggattt gggtgaagat aataacctta tgcaggcggc aaaactggtt
     2281 gagttattgg gcgaatcctc gcagatactg ttttttaccg gggcgggcat atccacgggc
     2341 agcgggatac ctgactttcg cgggccggac ggcgtgtgga aatatcggca accggtgtac
     2401 taccatgatt ttatgcggtc ggaagcggcg cggattgagc actgggattt caagctcgag
     2461 ggctgggagg cctttcgaac ggcgcgtccg aacgcagtcc acgaggccat tgtgcgtttg
     2521 gaacgcgcgg gcaaggtgcg ggcggtcgtg acgcaaaata tagacgggct gcacgcgcgg
     2581 gctggcacgg cggccggact tttaatcgaa ttgcatggca cgaaccgtct tattgaatgc
     2641 cagacttgcg ggcggcgatc ggagcccgag gcgcacttcg agtattttcg caagacccgc
     2701 cgcccgcccg tgtgtgagtg cggcggcttt ctgaaaccag cgacgattag cttcggccaa
     2761 aacctgcgaa tcgaagatct ggaaaaggcg gaagacatgg cccagggcgc tgatttggtc
     2821 gtggcgttgg gctcgacgct gtccgtccat cccgccgcgg atattccgct gctggcggcg
     2881 aggcggggcg cgccgtatgt gatcatcaat cgcgggccga cagagcacga tgagatgccg
     2941 gaggtgacgt tgcggatcga gggcgatgtg ggggagatct ttccgccggc cgttgcggcg
     3001 gcgctgaagg tttagcgcac tggttggttg aagtctggcg caaagcggca ttgttagccg
     3061 gccgggcggg caaccgcggg cgaggtttcg gcgcgaacga ttctgaacaa gaggcttttg
     3121 ctgtccctgg ttttggttca ttctgcgggc gtgaaaccgt tgtcggtgga aaaagttcgc
     3181 accgttgtcc gctccgcgct ggccgaagac ctgggccgcg gggatgtgac aactctggcc
     3241 acggtgccgg agagtgcgca tgcctgggcg gaaatccgcc cgcgtgaggc gatcgtggtg
     3301 gcgggcctgt cgctggccga agcggctttt cgggaaatca gcccggcggt gcgagtaaag
     3361 cgcgccaccg cggatggccg gcgcgcagcc gccggcgagc ctttgctcgt gattaacgga
     3421 cccgcccgcg ccctgctcag tgccgaacgc gtcgcgctta atttccttca gcgcctttgt
     3481 ggcatcgcca cgttaaccgc gcagttcgtt gcggccgtca ggggtacgcg cgcgaaaata
     3541 ctcgataccc gcaagaccac gccgggatgg cgctggctgg aaaaatacgc ggtcgcctgc
     3601 ggcggcggac ggaatcaccg tttcggtttg tttgatatgg tgctgattaa ggataaccac
     3661 ctggcggtgc tgaaggacaa aacgcccaat gccattgtgg ctgcggtgcg gcgagcgcgg
     3721 gcccgctatc cgaagctcaa ggtggaggtt gaagcggata cgctggagca ggtggcgcag
     3781 gcggtcgccg ccggggcgga cctggtcctg ctcgataata tgagcctgcc ccagttgcgc
     3841 cgggcggttg cgcagtgcaa agggcgcgcg ctgaccgaag ccagcggcgg ggtgactttg
     3901 cggcgcgtgc gcgcgctggc ccaaaccgga gtggattaca tctcggtggg ggcgctcacc
     3961 cattccgccc gcgcggttga catcgggctt gatttccgga gctgacggga acgcatacat
     4021 gagcctggac gtgcaaattc ttagtgcgct gcggggcccc ggcgatggcg cggtctcggg
     4081 cgcggaattg tcgcagaagc tgggagtgag ccgcgcggcg gtatgggcgc atatagagga
     4141 cttgcggcgg ctcggctacg acattgaggc gagtccgcac cgggggtatc ggttgttgag
     4201 cgcgccagac ttgctgcatg cggatgatat gcggtcgcgc ctggggccga cgcgggttat
     4261 tgggcgagac atccgggtat tcgaggagac tgcttcgact agtgatgtga tcgaaaagct
     4321 ggcgcgtgat ggtgttcggg aaggggtggt ggttttcgcg gaatcgcaaa cgcggggcag
     4381 gggacggctg gggcgaaaat gggtctcgcc ggctcgccgc gggttgtggt tttcggttct
     4441 cctgcgcccg gcggtgcggc cgcaggaagt cacacggctg acggtggcat cggccacagc
     4501 agtgcggcgg gccattgaat cgctgaccgg gctgaagccg aaaatcaagt ggccgaacga
     4561 catcctgatt cgagggcgga aggttgccgg catcctcacg gagttgagcg ccgaattgga
     4621 ctgcgtaagg cacgtggcgc ttggcattgg ggtgaatgtg aacctggagc ccggggatct
     4681 gcccccggaa ctgcgcgcgc agacgacctc cctgaagata gaatccagcc gaacagtttt
     4741 gcgaccggag ttggcagtgg caattttgcg cgaattggac cgcgattacg cccgcacggc
     4801 caccggccag tttgccgcgc tggcggagga atgggaagag catagcgcca cgatgggaca
     4861 ggaaatcgtc attcgcgtcg gcgatcgcca ggtccgcgga cgggcggagt cgttggatga
     4921 tgacggcgcg ctgcttttgc gctccgacca tgggcgcctg gaacgggttg tcgggggaga
     4981 tgtggcgctg gcaaaatgat tctcctgttt gacatcggca acacgcacac ccacctgggg
     5041 ctggcggatg cccggcgggt attgaaacag gcgaacctgc cgaccgtggc ctgggggcga
     5101 ggcagcccac gaggtgtgct tcagcgattt gcgggaggcg cacggttgga gggagcggta
     5161 ttatgcagcg tggtcccgcg tgcgaccgcc tgggttcacc aggcaatccg gcaactttgg
     5221 aatctgcctt gcctcgagct gacctcaaaa actctttgcg gcgtaggcat caactatccg
     5281 cgccccgcaa cgattggcgc cgatcgcttg gccaatgcgg ttgcggcgcg ccaccacttt
     5341 ggcgctccgg tggtggtggt ggacttcggg accgccgtga cgtttgatgt ggtggatcgc
     5401 gccggagatt atgttggtgg cattatcgct cccggactgt ccgcgatgac cgattacctg
     5461 cacgagaaga ccgcgttgct gccccgcatc cgtattcgtg aagttcaaag cgtgattggc
     5521 aagagcaccg agcaggccat gcgcgttggc gcagtgcaag gttatcgtgg cttgattcag
     5581 gagctgattc gggcgttgaa gcgtgaactc aaggcccccc gtcttcctgt tgtggcgacc
     5641 ggaggttgtg cggaactgat tggggccagg ctaaaagtga ttactgcggt ggatccgctg
     5701 ctgacgctcg aaggacttcg cttggagtgg caggcaaagc atcctgaccg gggggataag
     5761 tcaatgtcgg agcgggggcg cgcgcaaagg agtcaggaga cgccagctat ttgctgagtg
     5821 gcggttggtg attcaaaaag cggataatca tagtgcattg ggcaacgcga tgccgtcggt
     5881 cagccgttgg cattcccgtg ccgggttgtc gtacggcatt ggagcgtaca tggtgtgggg
     5941 ggttattccc ctctattttc atgtggtggc agaggtgcct ccctgggaga ttttgtgcca
     6001 tcggatagcc tggtcggccc tctttctggc gctggtggtg tcggttcgcg gtgaatggca
     6061 ggctctgcga cccatcctgc gcagccgtcg gaagatgttg ctcctctctg taggttccgt
     6121 cctcatcgcc ttgaactggc tgatattcat ttacgccgtc atcagccagc aagttctgga
     6181 agccagcctg ggctatttca tcaatccact gctatcggtt gcgcttggga tgatcttcct
     6241 tcgggagcgc ctgcaaggtt ggcagtggtt cgcagtgggc atcgccgggg ttgccgttgc
     6301 caacctggca tggcgaggct cgggctttcc ctggatcgcg gtttcgttgg ccggcacctt
     6361 tggtttctac ggtcttgtac gcaaggccgt gaacgtaaac tcgctacatg gtctgctggt
     6421 tgaaacagcg ctgctggtcc ccttggggtt gctggtgctg gctattgtgc ctgctcctcc
     6481 gcttccggtt ttgaccctgg gcgtgctgtc attgtcgggc attattaccg cggtgccgtt
     6541 gctgatgttt ggcgcggcgt tgcgccgatt gaaactttcc accatcggct ttctgcaata
     6601 cattggcccg acgctgcaat tgctggtcgc cacagcgatc ttccgagaac cgcttgaccg
     6661 aattaagctt gccagtttta tcctctgctg gttgggcata gcggtttata ccgctgattc
     6721 agttctctct aattccttgc agccggtagc tgatgagccg gaataatgca gaaaccgcaa
     6781 cccggctagc cgttggactt aaccgattcc ggacagccgt acgggagccg ccttcgggca
     6841 agttagccgg tttctgtact tgaggaactg ggttaatcag gcgaaggagc ttccagcaag
     6901 cggggttttc gttcgtgcag gggcagccag atgcgaaagg tagtgccgcg gtcttcccgg
     6961 ctttcgagtt cgaggtggcc gccgtgcgcc cggacgatac gctggacgat catcaggccc
     7021 aagccggtcc ccttttcctt ggttgtgtaa aaaggctcaa agatgcggtt gacttgttcc
     7081 tgttgaatgc cactgccggt gtccgcgacg ctgacccaga cgccatcggc cccttcgccg
     7141 gtttgcacgg tgagggtgcc gccccgggtc atcgcctgga tcgcgttttt aatgaggttt
     7201 accagaagtt gttggatttg agtcgcatcc aggggggcca gaggcagttg gggagccagg
     7261 cgggtgcgga gttgcaaacc gcggttatcc atttccacct gcagcaattg aagggttttc
     7321 tggacgactt cgttaagtga ggccggttta agctgtggct ggctaggtcg aatcgcctgc
     7381 aggaattggg taatgatgta gtcgagccga ttgatttcac cgcgcgcgac agacaggtat
     7441 tgctccagct tgggaacgag atcccgggca atgttgtcat ccgaggcatc ccgggatcga
     7501 gcgcgcaacc gaagacggga actggccgaa gccaggggct ggcttagggc ggctgagcgc
     7561 agcttcttga gttcgcgctc cataagttgc aaatggatgt ggagcgcatt gagtggattg
     7621 ccgatctcat gagccacact ggcggccagc agggtcagag cctggacccg ctcgctttcg
     7681 atgacttcaa tggttttctg gcgggcttcg gtggcatcat gcaggatgag agccactccc
     7741 gtgccgccgg cggcctcgcc tgcgagcggc gcggcataga gccgcagaaa acgcgggcgt
     7801 ggatagtgga tttcgaattc gtgccgaacg acgcccgagc catcggcgag gtcaaagtgg
     7861 cggatttttc cccattccag atcagggatg aagctggtga tgttctggtt ctcagctttg
     7921 ggttgcaagc cgaggaggcg ggtgacggct tggttgaaat agatgatgcg gccagattta
     7981 tccacaacca gcaccccgtc ttcaatggtg ttgaacaggg tttcgagaaa gctgcgctcc
     8041 cgggccaggc gctggaggac agtttgcagg ccggcttcgt ccaaccggcc gatgcggggc
     8101 aggactttgt cgaggaacct ggatttggca gccatggcag caagatttac ccgccggcca
     8161 ggagggcacg aattatcggc ggggtaaggc tgattccgga aggcccgagt tggtcaccca
     8221 agaggtccgc cgtgaagccg tcaggagcgg gatgcaaccg aaattgcggg attagaacca
     8281 tttcttttta cggaaatacc accagatagc cagggtggac agcagcgtga ccgcgaaagc
     8341 ggccaggtag ccctgcgtcc aatctacttc gggcatgttt ttgaagttca tgccgtacca
     8401 agtgccgacc aacatcatcg gagtggtgat gaccgtgatc atcgtcagca gcttgaccac
     8461 ttcactcgtg cggttggaag acatgttcaa aaagacatgc agaatagagg tgagggtgtc
     8521 ggcataaccc tgagccaatt ctgaaatatg gaagagcgta tcgtagatat cccgataata
     8581 gggaacgaga tgggcgcgga tgagtttgaa ctccccgcgc gcgaaacggg ccaatacctc
     8641 gcgctgcggg ccgatgatct gacgcaggtg gaggacctcc cgtttgactt gaataatctt
     8701 gttgaggata tggtggctgg gatcctgcag cgcctgttgt tcgagttcgg cgatttcaag
     8761 cgagagttcg tccagggccg gtttataatt atctacaatg gaatccagca gggtatgcgc
     8821 gacgcggtcg ggggcccggg caatgttggt gatggccttc accgcacgtt cttccgtttg
     8881 ggatacgctg cggagcggtc catcatgata agtgacgagg aagttcttgc cgagaaagag
     8941 actcagttcg ctggtggcga aaaccccatc cttgcggctg tagtctaccg catgaattac
     9001 caggaacagg tagggagcaa accaatcccc ctccttgggt gaatattctt ccaccttggg
     9061 cgaggggctc gcggtgacgc agtcctcgat ggacaatgga tggaaatgga agacgtcctc
     9121 caacacatat ttcgtctctt cggccgtggg gctttccaag tccacccaca gaaacaggtt
     9181 ggtgtcgctc agcaaggttg gcatcaagaa catctcgatg tccttgctat gcaggcggcc
     9241 ttgagttgta aataccagcg agcgtatcat gcaacagccc ttgtttgggc gaacagcatg
     9301 ttaggcggtc cgaagcgaaa agcaaggttg tgcgagattt atgtcgtgtg cgaaatttac
     9361 tgcgtccacg tcccaggcgg aatttatact gatttgccgg cctctcccgc aagctcgttc
     9421 aaacacgcaa taatatccgg taaaacaagg acacaatgct tgtcctgagc ggaggccccc
     9481 tggaatgctc ctctcgtcca gcaggtggtg gaatcgggcg gtaagagctg gtgagctgcg
     9541 ggtcgggaca gttagtgccc ggcgaatccc ggcaattcac actgatggca ttgccaccac
     9601 tgtagcccat cgtgggtcag ggcagagggt tgtgcccgga caaaccgggg cctccggtca
     9661 gccataatgt tcgtaaaata cgcctgcccc gacgaagcat ggataacctc gcaccagttc
     9721 gcaacgcacg cttgctgtgg cggagggttg atcagcctcg atggattgtc catgtcagcc
     9781 gtccgagcta tcaacgctgg caggtgcagt tcacgcattt atagcaaggt cccttgcgtt
     9841 ggcttggttc catctgggct ccttcacatg cctgatggct actgttgtga tcgcgggagt
     9901 ccgcggcgag cgtctttggc ggtggaagac ctcgtccgac taggcatttt ggcttacatc
     9961 atttcttcgg agcccaatcc attatgattc ttttcgctcc gtcccaccgg ctttcagctt
    10021 cttcgttcgg tgcggaccgc caatatcgac ttatatctcc attggcatag agctctattc
    10081 cttcatcgcc cccatttgct tgcagggatt ttagctgtcc ctcggggtaa caattcagct
    10141 taaagagcag tcttctcgac ttggagtctc gaacgattac ctccctcaca atgtagttcg
    10201 tgttccaaaa agtgaaaccg aacgtcttgt ctctctgtac gactgtggcc gagtgccggc
    10261 cattccataa ttgaacctct aagccatccg ctaaaacctt atctatgaca ttgcatattt
    10321 cgaccgccgc tttggcaaac ttctcagcct tcggggcatt agtcgcgccg cttgctctca
    10381 atgcaccctc tgctatcggc ttaagcacag tggata
//
LOCUS       DAOPQG010000122        10372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7327_length_10372_cov_16.181060, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000122 DAOPQG010000000
VERSION     DAOPQG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7327_length_10372_cov_16.181060"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            619..3447
                     /locus_tag="PKI07_11880"
     CDS             619..3447
                     /locus_tag="PKI07_11880"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HOC51783.1"
                     /translation="MGKALIIAEKPSVAGDIARALGGFEKQGDYYESERYVLSAAVGH
                     LLELCVPDQFEVKRGKWTFAHLPVIPPHFDLRPIDKSAARLKVLLKLIKRPDVASLIN
                     ACDAGREGELIFRYIVQYAKTRKPIQRLWLQSMTPAAIREGFAQLREDAAMRPLADAA
                     VCRSESDWLVGINGTRAMTAFNSRTGGFHLTTVGRVQTPTLAILVEREERIKNFVPRD
                     YWEVHGTFAAQAGEYPGRWFDERFAKKDGDPDLKPERIWDAAQAEAIRAKCLGQPGVV
                     TEESKPTTQLSPLLYDLTSLQREANGRFGFSAKATLGLAQALYEKHKALTYPRTDSRA
                     LPEDYLATVRSTLGMLEQTRYGAFASQILQREWVRPNKRIFNDAKVSDHFAIIPTSQA
                     PRHLNEQEAKLYDLVTRRFLAVFYPAAEFLVTTRITRVAGEPFKSEGKVLVNPGWLAV
                     YGKDAQPDDAPNLAPVQPGETVRTASVEVVAAQTKPPARFTEATLLSAMEGAGKLVED
                     EELREAMRDKGLGTPATRAQIIEGLLYEKYLVRQGRDLQPTAKAFSLIALLRGLAIPE
                     LSSPELTGDWEFKLRRMARGQMQRAEFMKEIADMTREIVAKAKQHESDTVPGDFGALK
                     TPCPKCGGEIRENYKKFQCQKCEFSLWKIVAGRQFEIPEVEELIARGRVGPLTGFRSK
                     QGRPFAAEIRMTPDLKPEFDFGPGKEDAEGEAAKADFTGQEPVGQCPKCRGRVFETPR
                     VYTCENSVGAARTCDFRSGKVILQRPIERAQMQKLLAAGKTDLLEKFTSRKGRLFKAY
                     LVLKEGRVEFEFEPRAAKSRARAAKPAPAAAPAPRLDFTGQQPLGPCPKCRQRVFEGD
                     AAYVCEQSQAARRPCKFKLNKVILQQPLDREQAARLLAQGRTDLLADFVSAKTGRPFK
                     AYLVMGETGKVTFEFPEREAEPKEAPAS"
     gene            3444..4406
                     /locus_tag="PKI07_11885"
     CDS             3444..4406
                     /locus_tag="PKI07_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOC51784.1"
                     /translation="MNAAAPEPPVPDAWIGKFLQHLAADRGASAYTQRNYRQALEEFC
                     RWRQAERLPPPGWEALQRDDFRAYVRFLGRQNLSRAAVQLRFSALRTFYRFLVRRGAV
                     AASPIRNLALPKVAKRLPRFLTPEQMADLLAAPLKLRPPPGQADSARAAAVLAARRDV
                     AVLETIYSCGLRISELCGLTAQDIDWHERVVRVRGKGKKERLVPIGEPALAAIRNYWA
                     LLPQAPAGDAPVFLAGPKKAAAIAPRQLQLRLKKHLAAAGLDPHLTPHKLRHSYATHL
                     LDAGADLRSVQELLGHAHLVTTQVYTHLTTERLKRAYDAAHPRA"
     gene            4490..6973
                     /locus_tag="PKI07_11890"
     CDS             4490..6973
                     /locus_tag="PKI07_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51785.1"
                     /translation="MMYPSPIWIRTWSVLAAGLLVIEMSGLARAALSAPVSRPIRAVL
                     REPLPPEELSLTEEAAEVVVAGPTFTYRVQKATGLVSAVRVVREGREVIASSGPAELQ
                     LDQYRFASEFNTGKLVVERAGKDQAVLRVEGTLRDPAQRGPEVDYTLRHTFFNDGVVV
                     SAVKLTPRGELRVERELVYRLSARGQLSHYLHKTRGENGSDAVRGSLPPAGQAVRFTA
                     ETSCLQVFSPAAALAIFTDGGALHLSQPKLDTAVVETAGPADGPGRISLAQYLIRIAP
                     GDPPRVLKAGEPLTFRVGLSVAPNRRPHPRMHDLRMFTWIGDEKHPYPTDAEISRVAQ
                     FGFTLFQMHRVGTPGLPRPPADEFMRVLDKVHELGLLFLWEENADLMYDSAPGVRELK
                     AKGQFALWQGFNYGGRYTAEMDSYCDLIATCLASPNGLAEYRLANLARMMDRYAVDGI
                     YLDDNLAYGNCTLAREHGHPREVYDCLIELHEMNWRRRELLRRRCPHTVLVSHSTRAF
                     VLPVVCNFDALIYGEGYSFGSLTDYWSYFGFAHCLPAQGMIWPGGQDIERCPASLVYN
                     YDLLTGGGQYSQIDWRLFPHKFPHAKGVTPPEPFYTQAYNLAQYDFGMYESRIWRFVD
                     AADLFAATAPQTFATVYRNQTWGDWLIPVANMANQDVKTALAFHSPEALGFEPHKDYV
                     LFNVHRRLARNLRGDALNAAFSDLAISGGSLQLFYLREAPGQAPFHLWGGKRISERWD
                     ASARKLTVSLDGPAGRQDTVFLAGANHGLQLVLAGGAPARFFFDPAQGLVHGQVTFTA
                     KPLTLEAHYSVSGTNRLPHKVAAPASPYF"
     gene            complement(7175..9493)
                     /locus_tag="PKI07_11895"
     CDS             complement(7175..9493)
                     /locus_tag="PKI07_11895"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOC51786.1"
                     /translation="MKLPTLHNFVNPLVLTHPGRAALVLLLALPGPPLASADANTCRL
                     ANDRLIIAFSPEHGDLVEFTDAHTRQNFAGTMPSGAPLWELTLAPANQTLTASSAQSF
                     RAQPLDRKPSGLRLTWEKFGLPAAPELRVEAVVRLDAGQPMSHWELAVSGLNALSLTH
                     VRFPRIINIPCQANERLAVPAWMGYLATEPRQVFAGPAGNGRRFQWSYPGHLSLQCLA
                     FYQQDGPGLYVACNDTAGFNKDFAFFGDRENRLHCEIAHLPERRPAAVPAWRLPYAVQ
                     LGTFTGDWFTAAERYRAWATNQPWARESRLARGRVSDWALNTALWVWNRGRSPGVLEP
                     AMVLREKLGLPVSVFWHWWHGCSYDAGFPEYLPPREGAESFTNALTRAQAKDIRTLVY
                     MNQRLWGMTTPSWTNEAAARFAVKGPDGQIHPEVYNTFTRQPCATMCMGTAFWRDKYA
                     GLAERAVQELGVNGIYMDQACTSLACFDPQHGHPVGGGVYWMKGFQALQADIRRRCAD
                     RREPVVLAGEGCGECWLPYLDLMLGLEISRERYAAPDGWDPLPFWPAVYHPFAITYGN
                     YASLTMPPYDDLWPAESAPAEPLKLLDRKYARQFCLEQARAFVWGQQPTLANFLPNQL
                     TERAEELDFVLRLARLRHRAENYLLRGTFLRPPRLQAPTATLDMSRLSIYAGREGGLT
                     AFQKTSPLALAAAWRAPDGKVGIALVSIADEPTRVTLHFPADRYGLPRRARVHLLDET
                     GRRQLGTLRQLGSEMEMELPPRAARILELAVP"
     gene            9779..9855
                     /locus_tag="PKI07_11900"
     tRNA            9779..9855
                     /locus_tag="PKI07_11900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9813..9815,aa:Arg,seq:tcg)
BASE COUNT         1778 a         3203 c         3604 g         1787 t
ORIGIN      
        1 taggatttta gctggtacac ccgatgggca tcccttgtac accccttgta cacgggcagg
       61 aggcaggtat ggtttagcct gaaaagactg gagttatgtg attggggagg caaaacggat
      121 ggaaagacgg tttgcagggc caggattcag ttcagtgtcc aaaaaatgga cagtatggat
      181 aagggcagaa aagaggagcg ggtgataggg tagggttgag cggccttatg gtttctggcg
      241 agggagaggg tattcgttcg gcggatttcg agtatgtcgg ggggattggg gttgtggacg
      301 gttttcagcc gtcgtccgga gggcttcgct ggcgtcacga agatattgcg ttttttcagg
      361 aagatgttaa ggtggggata tggctggata tcgcaggcca ctttcccgca gtgaattgcg
      421 gcaaataaga cgacagaagg ggcgcatcat tatttggtgc ggggtggcgg tgtttttagc
      481 tttgatttcg gtggttggcc tggtttgcct gatgtacgaa atgcccccgc tttgaccggc
      541 gcgcccggcg ggttcgcgtg ccgtcctccc gcattgattt gccacgaccg tggctaaagt
      601 gctagtgtgc cgggttatat gggcaaggca ttaattattg ccgagaaacc gtcggtggcg
      661 ggcgacatcg cccgggccct gggcggcttc gagaaacagg gcgactacta cgaaagcgag
      721 cggtatgtgc tttccgcggc ggtggggcac ctgctggagt tgtgcgtgcc ggaccagttc
      781 gaggtcaagc gcggcaagtg gacgtttgcg catctgccgg tcatcccgcc gcatttcgat
      841 ttgcgcccca ttgacaagag cgcggcgcgg ctcaaagtgc tgctcaagct gattaagcgg
      901 ccggacgtgg cctccctgat caatgcctgc gacgccgggc gcgaggggga attgatcttc
      961 cgctatattg tgcagtacgc gaagacccga aagccgatcc agcggctctg gctgcagtcc
     1021 atgaccccgg cggccatccg cgaagggttt gcgcaactgc gggaggacgc cgcgatgcgc
     1081 ccgctggcgg acgcggcggt gtgccgttcc gagtcggatt ggctggtggg catcaacggc
     1141 acgcgggcca tgacggcgtt caactcgcgg acgggcggat ttcacctgac gaccgtgggc
     1201 cgggtgcaga cgccgaccct ggccatcctg gtcgagcgcg aggagcggat taagaacttc
     1261 gtgccccggg attactggga ggttcacggc acgtttgccg cccaggcggg cgaatacccg
     1321 ggccggtggt ttgatgagcg gtttgccaaa aaggacggcg atcccgacct taaaccggag
     1381 cggatttggg acgccgccca agcggaggcc atccgggcca aatgtctcgg ccagccgggg
     1441 gtggtgacgg aggaaagcaa gcccacgacc cagttgtcgc cgctgctgta cgatttgacg
     1501 agtttgcagc gggaggccaa tgggcggttc ggtttttcgg ccaaggccac gctcgggctg
     1561 gcgcaggcgc tgtacgaaaa gcacaaggcg ctcacctatc cgcgcaccga ctcgcgcgcc
     1621 ctgccggagg actacctggc gaccgtccgg agcacgctgg ggatgctgga gcaaacccgc
     1681 tacggcgcct ttgccagcca gatcctgcag cgcgagtggg tccggcccaa caagcgaatc
     1741 tttaacgacg ccaaggtgtc cgaccacttc gccatcatcc cgacttcgca ggcgccccgg
     1801 cacctgaacg agcaggaggc gaagctgtat gacctggtca cccggcggtt cctcgccgtg
     1861 ttttacccgg cggcggaatt cctggtgacg acgcgcatca cgcgcgtcgc tggcgaaccc
     1921 ttcaagagcg agggcaaggt cctggtcaac ccgggctggc tggcggtgta cggcaaggac
     1981 gcgcagcccg acgacgcgcc gaacctggcg cccgtgcagc ccggggaaac ggtgcggacc
     2041 gccagcgtcg aggttgtcgc cgcgcagacc aagcccccgg cgcgcttcac ggaggcgacc
     2101 ctgctcagcg cgatggaggg cgcgggcaag ctcgtcgaag acgaagagct gcgcgaggcg
     2161 atgcgcgaca aggggctggg aacccccgcg acgcgcgccc agatcatcga gggcctgctc
     2221 tacgaaaaat acctggttcg ccagggccgc gacctgcagc cgacggcgaa ggcgttttcg
     2281 ctgattgccc tgctgcgcgg gctggccatc cccgagctgt cctccccgga attgacgggc
     2341 gactgggagt tcaagctgcg ccgcatggcg cgcgggcaga tgcagcgcgc cgagttcatg
     2401 aaggagatcg cggacatgac ccgcgagatt gtcgccaagg ccaaacagca tgagagcgac
     2461 accgtgcccg gggacttcgg cgccctgaaa acgccctgcc cgaaatgcgg cggggaaatc
     2521 cgcgagaact ataagaagtt ccaatgccag aagtgcgagt tcagcctctg gaagattgtg
     2581 gcgggccgcc agttcgagat tcccgaggtg gaggaactga tcgcgcgggg ccgggtcggg
     2641 ccgctgaccg ggtttcgcag caagcagggg cggccctttg cggcggagat ccggatgacg
     2701 ccggacctga agccggagtt tgactttggc cccgggaagg aagacgcgga gggcgaggcg
     2761 gcgaaagcgg attttaccgg ccaggaaccc gtcggccagt gtcccaaatg ccgcgggcgc
     2821 gtgtttgaaa cgccgcgggt ttatacgtgc gagaattccg tcggcgccgc gcgcacctgc
     2881 gatttccggt cgggcaaggt catcctgcag cgcccgattg agcgggcgca gatgcaaaag
     2941 ctgctggcgg cgggcaagac ggatttgctc gagaagttca cctcccggaa ggggcggttg
     3001 ttcaaggcct acctggtgct caaggagggc cgcgtggaat tcgagttcga gccgcgggcg
     3061 gccaaatccc gcgcccgcgc cgccaaaccc gcccccgccg ccgcgccggc gccccggctt
     3121 gactttacgg gccagcagcc gctgggcccg tgccccaaat gccgccagcg ggtgtttgag
     3181 ggcgacgcgg catacgtctg cgaacagtcc caggccgccc ggcggccgtg caaattcaag
     3241 ctcaacaaag tcatcctgca acagcccctg gatcgggagc aggccgcccg gctgctggcg
     3301 caaggccgta cggatctgct ggcggacttc gtttccgcca aaaccggccg tcccttcaag
     3361 gcgtacctgg tgatgggcga gacgggcaag gtcacgttcg aatttcccga gcgcgaggcc
     3421 gaaccgaagg aggccccggc gtcgtgaacg cggcggcccc ggaaccgccg gtcccggatg
     3481 cgtggatcgg gaagttcctg cagcacctgg cggcggatcg cggggcctcg gcgtacacgc
     3541 agcgcaatta ccggcaggcg ctggaggagt tctgccgctg gcgccaggcg gagcgcctgc
     3601 cgccgcccgg gtgggaggcg ttgcagcggg atgatttccg cgcgtatgtg cgcttcctgg
     3661 ggcggcagaa cctgagccgc gccgcggttc agcttcgttt cagcgccctg cggacctttt
     3721 accggtttct cgtgcggcgg ggggctgtgg cggcgtcgcc catccggaac ctggccctgc
     3781 ccaaggtcgc gaaaaggctg ccccggtttc tcacgccgga gcagatggcc gatctgctgg
     3841 ccgccccgct caagctccgg ccgccccccg ggcaagccga ttccgcccgc gcggcggccg
     3901 tcctcgcggc ccggcgcgat gtggcggtgc tggaaaccat atattcctgc ggcctgcgca
     3961 tcagcgagct gtgcgggttg acggcgcagg atattgactg gcatgaacgc gtcgtgcgcg
     4021 tgcgcggcaa aggcaaaaag gagcggctcg ttcccatcgg cgaaccggcc ctggccgcca
     4081 ttcgaaacta ctgggcgctg ctgccccagg cgccggccgg tgacgccccg gtttttctgg
     4141 cgggaccaaa gaaggccgcc gccatcgcgc cgcggcaact ccaattgcgg ctgaagaaac
     4201 acctggcggc cgcgggtttg gatccgcacc tgacgcccca taagctgcgg catagctatg
     4261 ccacgcacct gcttgacgcg ggcgcggacc tgcgcagcgt tcaggaactg ctcgggcatg
     4321 cacacctggt cacgacccag gtctataccc acctgaccac ggaacggctc aagcgcgcct
     4381 acgacgccgc ccacccgcgg gcctgaaccg gcggcgcccc ggcgggcggg ccgggggcgg
     4441 ggcgcgcaaa atactggcaa tttgccggcg ggtcattagc ttgagccgca tgatgtaccc
     4501 gagcccgatt tggatccgga catggagcgt gctggccgcg gggttgctgg tgattgaaat
     4561 gtcggggctg gcgcgcgccg ccctttccgc tcccgtcagc cgccccatcc gcgccgtcct
     4621 gcgcgaaccg ctgccgcccg aggagttgag cctcacggaa gaggccgccg aggtggtggt
     4681 ggccggcccc acctttactt accgcgtgca aaaagcgacc gggctggtca gcgccgtccg
     4741 ggtggtgcgc gaggggcggg aggtcatcgc gtccagcggc ccggcggagc tccagttgga
     4801 tcaataccgg ttcgcttcgg aattcaacac cggcaagctg gtcgtcgagc gcgcgggtaa
     4861 agaccaggcg gtcctgcggg tggaggggac gctgcgcgac ccggcccaac gcggtccgga
     4921 agtggattac acgctgcggc atacgttctt caatgatggc gtggtggtga gcgcggtgaa
     4981 gctgacgccg cgcggggaat tgcgcgtgga gcgggagttg gtgtaccggc tttccgcgcg
     5041 gggccagctc agccactacc tgcacaagac ccggggcgaa aacggcagcg acgcggtgcg
     5101 gggcagcctg ccgcccgccg gccaggcagt tcgttttacg gcggagactt cctgcctgca
     5161 ggttttcagc ccggcggcgg cgctggccat ttttaccgac ggcggcgcat tgcacctgag
     5221 ccagccgaag ctggacacgg cggtggtgga aaccgccggg cccgcggacg gcccggggcg
     5281 gatttccctg gcgcagtacc tgatacggat cgcccccggc gacccgcccc gcgtgctgaa
     5341 agcgggcgag ccgctcacct tccgggtcgg cctcagcgtg gcccccaacc ggcggccgca
     5401 tccccggatg cacgacctgc gcatgttcac ctggatcggg gatgagaaac acccttaccc
     5461 gacggacgcg gagatcagcc gggtcgccca gtttggtttt acgctgttcc agatgcaccg
     5521 cgtggggacg cccggcctgc cgcgcccgcc cgccgacgag ttcatgcggg tgctggacaa
     5581 ggtgcatgag ctgggcctgc tttttctgtg ggaggagaac gcggatttga tgtatgacag
     5641 cgcgcccggg gtgcgggagt tgaaggccaa aggccagttc gccctctggc aggggttcaa
     5701 ttacggcggc cgctacaccg cggagatgga ctcctactgc gacctcatcg ccacctgcct
     5761 ggcttccccg aacggcctgg ccgaataccg cctggccaac ctcgcgcgca tgatggaccg
     5821 gtacgccgtg gacggcattt acctggacga caacctggct tacggcaact gcacgctggc
     5881 gcgcgagcat gggcatccgc gcgaggtcta tgattgcctg atcgaattgc atgagatgaa
     5941 ctggcgccgc cgcgagctgt tgcgccgccg ctgcccgcat acggtgctgg tcagccacag
     6001 cacgcgggct tttgtcctgc cggtagtgtg caatttcgac gcgctgatct atggcgaagg
     6061 ctattccttt ggttcgctga cggattactg gagctatttt ggctttgccc attgtcttcc
     6121 cgcccagggc atgatctggc ccggcggcca ggatatcgag cgctgcccgg cgtcgctggt
     6181 ttataactac gacctgctca ccggcggggg ccaatacagc cagattgact ggcgcctctt
     6241 tccgcacaag tttccgcatg ccaaaggcgt cacccccccc gaacccttct acacccaggc
     6301 ctacaacctg gcccagtatg acttcggaat gtacgagtcc cggatctggc ggtttgtgga
     6361 tgccgccgac ctgttcgcgg ccacggcgcc ccaaacgttc gcgacggtgt atcgcaacca
     6421 gacgtgggga gactggctga ttcccgtcgc caacatggcg aaccaggacg tcaagaccgc
     6481 cctggctttt cattcccccg aggcgctggg attcgagccg cataaagact acgtgctgtt
     6541 caacgttcat cggcggctcg cgcggaattt gcggggcgac gctctgaacg cggcgttcag
     6601 cgacctcgct atttccgggg ggagcctgca gctcttttac ctgcgtgaag cgccagggca
     6661 agcgcctttc catctgtggg gcggcaagcg aatctcggag cggtgggatg cctcggcccg
     6721 caagctcacg gtgagcctgg acggcccggc ggggcggcag gataccgtgt tcctggccgg
     6781 ggccaaccac ggtctgcaat tggttctggc cggaggcgcg ccggcgcggt tcttcttcga
     6841 cccggcccag gggctggttc acgggcaggt gaccttcact gccaaacccc tgaccctcga
     6901 agcgcactat tccgtcagcg gcaccaaccg gttgccccac aaggtggcgg cgcccgcaag
     6961 cccgtacttc taaccgccgg atttcagctt ccgaaggaac cggcaaccgc ccgccgcgcg
     7021 aaggcgcgcc gcctgcaagc gtctttcctt ttccccgctg accgcctgcc tgcgggcggc
     7081 gtggtacggg cgaaacccgc gggcgggaaa cgcttttgcc cgttcgccgg gcgcggcggg
     7141 ctgccggggc gggtctttga tgggtgctcg gcgcttacgg aaccgccagt tccaggatgc
     7201 gggcggcccg ggggggcagc tccatttcca tttcggagcc gagctggcgc agggtgccaa
     7261 gctgccgacg gccggtttca tccagcagat ggacccgggc gcggcgcggc aggccgtagc
     7321 ggtcggcggg gaagtggaga gtgacgcggg tgggttcgtc cgcgatgctg acaagcgcga
     7381 tgcccacctt gccgtccggc gcccgccagg cggcggccag cgccagcggg ctggttttct
     7441 ggaatgccgt caggcctccc tcccgcccgg cgtagatcga gaggcgcgac atgtccaggg
     7501 tggccgtcgg ggcctggagc cgcggggggc gcaggaaggt tccgcgcagg agataatttt
     7561 cggcgcggtg gcggaggcgc gccaggcgca gcacaaagtc caattcctcg gcgcgctcgg
     7621 tcaactggtt cgggaggaaa ttggccaggg tgggttgctg cccccagaca aaggcccgtg
     7681 cctgttcgag gcagaattgg cgggcatatt tccggtcgag gagcttgagc ggctcggcgg
     7741 gcgcggactc tgccggccac aggtcgtcgt agggcggcat ggtcagcgaa gcgtagttgc
     7801 cataggtgat ggcgaaagga tggtacaccg ccggccagaa cggaagggga tcccagccat
     7861 cgggggcggc gtagcgttcg cggctgattt ccaggccgag catcagatcg aggtagggca
     7921 gccagcattc gccgcaaccc tcccccgcca gcaccaccgg ctcgcggcgg tcggcgcagc
     7981 ggcggcggat gtccgcctgg agcgcctgga accctttcat ccagtaaacg ccgccgccca
     8041 cggggtgtcc gtgctggggg tcgaaacacg ccaggctggt gcaggcctgg tccatgtaga
     8101 tgccgttcac gcccagttcc tggaccgcgc gctcggccag gccggcgtat ttatcccgcc
     8161 agaaggcggt gcccatgcac atcgtcgcgc agggttggcg ggtgaaggtg ttatagactt
     8221 ccgggtgaat ctggccgtcc gggcctttga cggcgaaccg cgcggcggct tcattggtcc
     8281 agcttggggt ggtcatgccc cagagccgct ggttcatata caccagggtg cggatgtctt
     8341 tggcctgcgc gcgggtcaag gcgttggtga aactctcggc gccctcgcgg ggcggcaggt
     8401 attccgggaa accggcgtcg taggagcagc catgccacca gtgccagaag acgctgaccg
     8461 gcaggcccag tttttcccgc agaaccatcg ccggttcgag cacgcccggc gagcggccgc
     8521 ggttccagac ccacagcgcg gtgttaagcg cccagtcgga cacccggccg cgcgccagcc
     8581 ggctttcccg ggcccagggc tggttggttg cccaggcgcg gtagcgctcg gcggctgtga
     8641 accagtcgcc ggtgaaagtc ccgagttgga cggcgtaagg caggcgccag gccgggaccg
     8701 ctgccgggcg gcgctcgggc aggtgcgcga tttcgcagtg caggcggttt tcgcggtcgc
     8761 cgaagaacgc aaagtctttg ttgaacccgg cggtatcgtt gcaggccaca tacagccccg
     8821 gcccatcctg ttggtaaaac gccaggcatt gcagggacag atggccggga tagctccatt
     8881 ggaaccgccg cccgttcccc gccgggccgg caaagacctg gcgcggctcg gtggcgagat
     8941 accccatcca tgccggcacg gccagccgct cgttggcttg gcacgggatg tttatgatgc
     9001 gcggaaagcg gacgtgggtg aggctcaatg cattcagacc gctgaccgcc agttcccagt
     9061 ggctcatggg ttgcccggcg tccagccgca ccaccgcttc cacgcgcagt tccggcgccg
     9121 ccggcagtcc aaacttttcc caggtcaggc gcagcccgga gggcttgcgg tcaagcggtt
     9181 gggcgcgaaa ggactgcgcg gatgacgccg tcagggtctg gttggccggc gcgagcgtta
     9241 attcccagag cggcgctccg gagggcatcg tgcccgcaaa gttctggcgc gtatgcgcgt
     9301 cggtgaactc caccaggtcc ccgtgctcgg ggctgaaagc gattatcagc cggtcattgg
     9361 cgagccggca ggtgttggca tccgcagagg caaggggggg gcccggcagc gcaagcagca
     9421 gcaccagggc cgcgcggccc ggatgggtca gcaccaaagg gttcacgaag ttatgaagtg
     9481 tgggcagttt catctggccg caaggctggc tcaaacggaa ggcctgaaca ataaaaaacc
     9541 ggcggcgggc agttggggcg gggaaggttc tttctggggc ggcgtgcgca ccgcaggctg
     9601 cattggcgcc gccgggaacc gcagtcgccg gccttttcct gctcgacttg gggcgccacc
     9661 atcgcttacg ttgcgccttg ccgtgggcat gcctggcgca ttatccgaat gggggcggaa
     9721 aagtgggacg agatagcgaa gagcgtgtct aactttccca agcgcaggct ttgcgggtgc
     9781 gtccgtagct caattggata gagcttctga cttcggatca gaaggttgca ggttcgagtc
     9841 ctgccggatg cgccaacttt aagtgccgta aaatcaagtg gttccgattc tggccgcaga
     9901 gcccaaaaaa agcacgaaag acgtgcttgg ccaattcttg gccaattttt gggttctgcc
     9961 tctgcacacg cggtatgacg atccccctgg ggaaggcgaa aggaatcctg aatctgtcgt
    10021 cccgcagtca agaaagtcgc ggcgggtttc ccccattcaa gtagcggtga atttcggtgc
    10081 gcggaatcag catacgccga aggccgggaa gcttccgaag cactttacgg cgaacgaggc
    10141 ggcggattgt ttcttcgcaa acgcccagtt cagcagcggt gcttttcaca tcgagggttt
    10201 ggcattcggt tgttggtgtc gcattcatga cgaggcaaca cactacaact catcgcgact
    10261 tctttggtgt agctgccgca caaccccgat cacgagaaac agaggccgcc gcgaattcta
    10321 atccggaacg attcagttga aaaagtaagc accgtttggc ggacttggga gg
//
LOCUS       DAOPQG010000123        10356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7344_length_10356_cov_14.637220, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000123 DAOPQG010000000
VERSION     DAOPQG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7344_length_10356_cov_14.637220"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1911
                     /gene="pgm"
                     /locus_tag="PKI07_11905"
     CDS             229..1911
                     /gene="pgm"
                     /locus_tag="PKI07_11905"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HOC51787.1"
                     /translation="MSLAMVSSNTNKPMGVSPLAGKLIPREKLVDLKQLAREYYHQKP
                     DPGNRNQAVSFGTSGHRGSPLRGTFTEAHVLAITQAICDYRRCQGIRGPLYMGKDTHA
                     LSGVAQRSALEVLAANNVEVIIQRNDGVTPTPVISRAILVYNRNRKEALADGIVITPS
                     HNPPEDGGFKYNPSNGGPADTGVTKWIERRANELLRDGNAGVSRIPFDRALSAATTHK
                     EDLVLAYVQDLRNAIDMDAVHAGGLRLGVDPLGGASLPFWEMINSIYKLDLTVVNPRL
                     DPTFSFMPVDHDGKVRMDCSSAYAMAGLVGLKEHYQVAFGNDPDADRHGIVTPSAGLL
                     NPNHYLAVAIHYLLSHRPNWNPKAAVGKTLVSSSLIDRVVRKLGWTLIEVPVGFKYFA
                     SGLFEGSFCFGGEESAGASFLRREGTVWTTDKDGILLALLAAEITACTGKDPGEHYQM
                     LTMELGVPFYTRIDAAITPEKKARLAKLSPEAVKAPSLAGETIIARLTHAPGSHEPFG
                     GLKVVAANGWFAARPSGTENLYKIYAESFCDEKHLEAIVHEAQNIVSLALND"
     gene            1955..3595
                     /gene="pgi"
                     /locus_tag="PKI07_11910"
     CDS             1955..3595
                     /gene="pgi"
                     /locus_tag="PKI07_11910"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOC51788.1"
                     /translation="MNPTVSPKFLVERDAWKTLAVHFRTARQWHLRDLFANDPQRGER
                     LNAEAAGWFLDYSKNRITDETIKLLLQLARESRLSERIEAMFRGDKINFTEDRAALHV
                     ALRAPRSESMLVDNRNVVPEVHAVLDRMADFADRVRCGTWKGYTGKPIRNLVNIGIGG
                     SDLGPVMAYEALKHYSTRTITFRFVSNIDGSDLEEAARGLDPAETLFIVSSKMFNTLE
                     TIANARSARQWLLNGLGADEKAVAKHFVAVSTQAQRVADFGIDTTNMFGFWDWVGGRY
                     SMGSAIGLSTMIAIGPEHFRAMLDGFRQMDEHFRREPFERNLPVIMALLNIWNNNFFG
                     APAVAVLPYEQYLKRFPAYLQQLTMESNGKNVTLDGQAVNYDTCPVYFGEPGTNGQHS
                     FYQLIHQGTRLIPCDFIAFAQELVPLGHHHELLLANAFAQGEALAFGLRPEEVRDTPS
                     WLVPHRVFTGNRPSNTLLAERLTPTTLGALVALYEHVVFTQGVIWNINSFDQWGVQLG
                     KVLAQNIAAELESTGDPELKHDSSTNNLICRYRRLKKR"
     gene            3642..4397
                     /gene="pgl"
                     /locus_tag="PKI07_11915"
     CDS             3642..4397
                     /gene="pgl"
                     /locus_tag="PKI07_11915"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOC51789.1"
                     /translation="MFTACSWPPNGYETSETALAGIMEIRVQPDVEAVAAAAAREIAC
                     TAREAVAARGRFVLAVSGGRTPWKMLRALTCEQVPWDRVYVVQVDERVAPPEHPDRNL
                     VHLRESLLGCVSLLAKQIQAMPVDFPDLETASRNYAHVLQGIAGIPPVLDLVHLGLGA
                     DGHTASLIPGDPVLQVTEADVAPTQPYQGRRRMTLTYPILNRARRILWVVTGADKAEM
                     LLRLCEGELTIPASRVRRDRTLVLADPAAAGKL"
     gene            complement(4547..5530)
                     /locus_tag="PKI07_11920"
     CDS             complement(4547..5530)
                     /locus_tag="PKI07_11920"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOC51790.1"
                     /translation="MRPHPRIIDRIIAGLQESRTICVAGHIRPDGDCVGSQLGLTLAL
                     RNAGKQVVCWNEDRIPQKYEFLDADGIIQKPMPGLEFDCVVAVDAATFDRLGSVGQYI
                     SRRKLFINIDHHESNSRFADLNWVSVREPSTGELVYRLLKIAKWPITRRIADCLFTAV
                     STDTGSFQYASTQPGTYHVAGELVSRGANLARICNEVYQSYPLSRARLLRHIYSHFRL
                     AHQNQIAYFWLKKADLTRTGADSSDTEGLIDHIRAIAPVVVACVFEEVEPELTRVSLR
                     SKSAKVNVSEIAAQFGGGGHSAAAGARILGKPLSVQRKVIGAVRRMLNSIH"
     gene            complement(5577..5942)
                     /gene="rbfA"
                     /locus_tag="PKI07_11925"
     CDS             complement(5577..5942)
                     /gene="rbfA"
                     /locus_tag="PKI07_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOC51791.1"
                     /translation="MVSLRLQRVRELLKREIGEVIRREFQVSEAGLITVNDVEVAGDL
                     KSAFVFISILGSADQQRQGLSLLRQHRRRIQELVAGAVILKYTPRLRFLVDDSIARGN
                     RVLEIIEELEKTLPEEPPE"
     gene            complement(6052..8568)
                     /gene="infB"
                     /locus_tag="PKI07_11930"
     CDS             complement(6052..8568)
                     /gene="infB"
                     /locus_tag="PKI07_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOC51792.1"
                     /translation="MPVRIYEIAKKLGWESKQVLAKAKSLGITTARVASSSLDKITAE
                     YLELEILKEHPELAEPAPVSAQETSPIPVEQPTETAAAPPVIEIKPSVEPEVAEAVVP
                     PVIEELPAIPAPEAEVADVPSEPSLAPQVVAEVQPQPQLESAVVEPPPPPVFKVGDKV
                     GFIQLRPKPPPKGSERSGAQKAPPRGASPGKKADFFPREGGSGGRTGTVASGTTGGKG
                     VGVTKGAVAAKASAEAKLQLPLDAQVITIKPPIVVRDLAEQLKQRPFKIIADLMELGV
                     LASVNQAIDEKEAQRICAKYGYRFEAERRVRDGGLHKPIGPTIDQDPEDKAEDLIPRP
                     PVVTIMGHVDHGKTTLLDVIRKANVVASEAGGITQHIGAYTISFPHPDHKKALAQVTF
                     LDTPGHAAFSSMRARGANVTDVVVLVVAANDGVMPQTLEALSHAQAAKVPVLVAVNKI
                     DHPNANALRVRQQLQDKGLVPDDWGGDTIFVDVSALTKQGVDKLIEMILLQADLLELK
                     ANPNRRAKGNVIESGLEPGGPTATVLVRKGTLRVSDVVLCGQYYGRVRALINEESKRL
                     KEAAPSVAVKILGLNGVPEAGLEFNVVENEKAARELAEQRILEARALGQEPARAKVTL
                     ENLFDRLDSSSGKELKVVVKADTQGSVEAIVDALKKIESDKVSLEVIHSAVGTITESD
                     VALASASNAVILGFHARIDSGVADRAKHEGVQIKQYAIIYELIDQVKEAMAGLLEPLV
                     KDITVGAAEVRKIFELSKGAPVAGCMVVSGRIAKGKVRVRRRKEIIYEGIVQSLRRFQ
                     DEVNEVRAGMECGIRIEGFREFQIGDSIECYTMEKIAQKL"
     gene            complement(8668..9948)
                     /gene="nusA"
                     /locus_tag="PKI07_11935"
     CDS             complement(8668..9948)
                     /gene="nusA"
                     /locus_tag="PKI07_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOC51793.1"
                     /translation="MNADFLAVLEFWEREKGLSREVLTAAVQEALLSAAKKAVGPARE
                     LRVDIDQKSGDIRAFAKLQVAERVISRHGQIALFDARRIKADAQVGDELEVEVTPVGF
                     GRIAAQYARQALMQHIRRAEKALIFTEFKDRVGDVVSGAVRRFDRSDVFVDLGKYEAL
                     LPNRERVPTEEYQLGERIRCYVKAVEEGPHGPEIILSRSDPRFVVKLFQIEVSEINDG
                     TIEIKGIAREPGFRTKLAVWTRDEKVDPVGACVGLRGQRVKNIVRELNNEKVDIIRWD
                     ANIKNFITNALAPAKLKAFEIDEAHKRVKITVSADQLSLAIGKRGQNARLTSKLTGWQ
                     VDIEPEVVVTKGFEEKVAEAVDSLAAITGITREQADVLVHHGLTRLEDLLQADESDLA
                     EIPGLGDQGAAILEAARAEAARRTLKVGETPISN"
BASE COUNT         2245 a         3024 c         2736 g         2351 t
ORIGIN      
        1 cccagcatac cacgccaaac gacattgtaa agagttatct gttacataac actagttttt
       61 tgggatgaaa tgagggcagg cagttgaacc acccaactga atcgctgcgg tttcgtttag
      121 ttggttttcg aaagtccaca ggagtttgaa agttcagcgt atccggcaaa tcaggttaaa
      181 gttagtttgg gacaacagtt aatattgaat cggcgtagat ttccctttat gagtctcgcg
      241 atggttagca gcaatacaaa taagccgatg ggagtttcac cgctcgctgg caaactcata
      301 ccgcgggaaa aactggtgga cctcaagcaa cttgcgcgcg aatattacca ccagaaacct
      361 gaccccggca accgcaacca agcggtcagt ttcggcacgt cggggcatcg tggcagtccg
      421 ctgcgcggga cttttaccga agctcacgtt ctagcgatta ctcaagcgat ttgtgattat
      481 cggcgttgcc aaggtatccg tgggccgctg tacatgggca aggatacaca tgcgctttcg
      541 ggggtggccc aacgctccgc cctcgaagta ctggccgcca ataatgtcga ggtgatcatc
      601 cagcgcaacg atggcgtaac ccccactccg gttatctcgc gggctattct ggtttataac
      661 cggaaccgta aggaagcctt ggcggatggt attgttatta caccatcgca taacccaccg
      721 gaggatggcg gatttaagta taatccgtca aatggcggcc cggcggacac aggggttacg
      781 aaatggattg aacggcgggc taatgaactg ctacgcgacg gcaatgctgg tgtgagccgg
      841 atcccattcg accgggcact ttcggcggca accactcaca aggaagatct cgttttagcg
      901 tatgtccagg atttgcgtaa tgccattgac atggatgcgg ttcatgcggg cgggcttcgg
      961 ttaggggtgg acccgctggg cggggcctca ctgccgttct gggaaatgat aaactccatc
     1021 tataagttgg acctcaccgt ggttaacccg cggctggatc cgaccttttc attcatgcca
     1081 gtggaccatg atggtaaagt tcgtatggat tgttcaagtg cttacgcaat ggccggattg
     1141 gttggattga aggagcatta ccaagtggct tttggcaacg acccggacgc tgatcggcat
     1201 ggcattgtta caccgtcggc agggttgttg aatccaaatc actatttagc agtggccatt
     1261 cactatttgc tcagtcaccg gccaaactgg aatcctaagg ctgcggtggg caagacgctg
     1321 gtcagcagta gcctgattga tcgcgtagtg cgaaaactgg gctggacgtt gatcgaggtg
     1381 ccggtgggat tcaagtattt tgcatcgggg ctgtttgaag gctccttttg ttttggcggc
     1441 gaggaaagtg caggggccag ctttttgcgg cgtgaaggaa cagtttggac tacggataaa
     1501 gatggcattt tgctggcttt gttggctgca gagattacgg cctgcaccgg gaaggatccg
     1561 ggagagcatt atcagatgct cactatggaa cttggggtgc ccttctacac gcgaattgac
     1621 gctgcaatca ccccagagaa gaaagcgcgc ctggcaaaac tatcacccga agcggtcaag
     1681 gcgccgagcc tggcgggtga aaccatcatt gccaggctga cgcacgcgcc gggatcccat
     1741 gaacccttcg gtgggttaaa ggtggtggcg gccaatggtt ggttcgccgc ccgaccttct
     1801 ggcaccgaaa acctttacaa gatttatgcc gaaagtttct gcgatgagaa acacttggaa
     1861 gctattgtcc atgaagcgca gaacattgtc agccttgctt tgaatgacta aaaggccgcc
     1921 ggccttcgct taacggccgg ggcgatcaag aatcatgaat cctactgtta gccccaaatt
     1981 tcttgtagag cgcgatgcct ggaagacgct ggctgtgcat tttcggactg cccgccaatg
     2041 gcatttgcgg gatctcttcg ctaacgatcc gcagcgcggg gaacggctaa atgccgaagc
     2101 cgcaggatgg ttcctggact attccaagaa tcgtatcacg gacgaaacga ttaaacttct
     2161 cctgcaactg gcgcgggaat ccagattgtc cgaacgcatt gaagcgatgt tccgcgggga
     2221 taagattaat ttcaccgaag atcgcgccgc cctgcacgta gctttgcgcg cccccaggag
     2281 tgagtccatg ctcgtggaca accggaatgt ggtgccggaa gtgcatgcgg ttctcgaccg
     2341 gatggctgat tttgccgatc gcgttcggtg tgggacctgg aaggggtaca ccggcaaacc
     2401 gatccgtaat cttgtcaaca ttggcattgg aggctccgac ctcggcccgg tgatggccta
     2461 tgaagccctc aagcattaca gcacccggac tataaccttc cgatttgtct ctaacataga
     2521 tggaagcgac ttggaggaag cggctcgggg cttggatccg gcggagacgc tgttcatcgt
     2581 gtcctccaag atgttcaata cactggagac tattgcaaat gccagaagcg cccgtcagtg
     2641 gctgctcaat ggattgggtg cggatgaaaa agcggtcgca aaacattttg tcgccgtttc
     2701 cacccaagcc cagcgtgtgg ctgactttgg catagatacg accaacatgt ttggcttttg
     2761 ggattgggtt ggcggccgct actccatggg atcagcgatt ggcctttcca caatgatagc
     2821 cataggcccg gagcacttcc gcgcgatgtt ggacggcttc cgccaaatgg acgaacattt
     2881 ccgccgggaa ccgtttgaga ggaatctgcc ggtgatcatg gccttgctga acatctggaa
     2941 caacaacttc tttggagcgc cggcggtggc ggttctgcct tacgaacagt acctcaaacg
     3001 ctttccggct tacttgcaac agttgaccat ggaaagcaac ggcaagaacg tcaccttgga
     3061 tggccaagcg gtaaattatg atacttgccc cgtctatttt ggcgaaccgg gaaccaacgg
     3121 gcagcactca ttctaccaat tgattcacca aggaacgcgg cttattccgt gcgatttcat
     3181 cgcatttgct caggaactcg tgccgctagg gcatcatcat gaactgctac tggcaaatgc
     3241 ctttgcgcaa ggtgaggcgc tggcttttgg cctgaggcca gaggaggtcc gggacacgcc
     3301 gagttggctc gtaccacacc gggtattcac gggcaatcgc ccgtcaaaca cccttctggc
     3361 tgaacgcctc acgcccacca cgctgggtgc gctcgtggca ctctatgaac atgttgtttt
     3421 cacccaaggc gtaatttgga acatcaactc tttcgaccaa tggggcgtcc aactcggcaa
     3481 agtgctggcg cagaatatcg ctgctgaact ggaaagtacg ggtgatccgg agctgaagca
     3541 tgacagttcc acaaacaatc ttatttgtcg ctatcggcgg ttgaaaaagc gttagagcgg
     3601 tttaaactaa actgtggccg ccgcagaatc tttaatcgcc aatgtttaca gcctgttctt
     3661 ggccaccaaa cggctacgag accagtgaaa ccgctctagc cggcatcatg gaaattcgtg
     3721 tgcaaccgga cgtcgaggcg gtcgccgccg cggcggccag ggaaatcgcc tgcacggcta
     3781 gggaagccgt ggcggcgcgg ggtcgattcg tcctggccgt cagcgggggc cgcacgcctt
     3841 ggaaaatgct gcgtgcgtta acgtgcgagc aagttccgtg ggatcgcgtc tacgtggtgc
     3901 aggtagatga acgggtggca ccaccggaac atccagaccg aaatcttgtt cacttgcgtg
     3961 agagtttgct ggggtgcgtc tcgctgctcg cgaaacaaat ccaggctatg cctgtggatt
     4021 ttccggacct cgaaacggca tcccgaaact acgctcacgt actgcaggga atcgccggca
     4081 ttccgccagt tcttgacctc gtccacctgg gccttggcgc ggacgggcac accgcgtcat
     4141 tgataccggg cgatccggtg ctccaagtca cggaagcgga cgtggcacca acccaacctt
     4201 accagggccg tcggcgaatg acgttgacct atccgattct caatcgcgca cgacgcatcc
     4261 tgtgggtggt gacgggcgcg gacaaagcgg agatgctgtt acgattgtgt gagggagagc
     4321 tgacgatccc ggctagccgt gtgcgccgcg accgaactct ggtattggcc gatccggcgg
     4381 cagcgggaaa gctctgaaat tcgtcaggca gccgcgatga ggtcatatcg cacctcgcca
     4441 aggggttcca ttcgttaccg tttctataaa gactcgttct ccatgggtag gaccatccct
     4501 accggcccac acgaacaata ggtacgtgtt aagaaacggc gtcgaatcag tgaatcgagt
     4561 tgagcatccg cctgactgct ccgatgacct tccgctgcac ggaaaggggt ttgccgagaa
     4621 tacgcgctcc agccgctgcg gagtgtcctc cgcctccgaa ttgagcggcg atttcactta
     4681 cattcacctt cgcacttttt gagcgcaggc tgacccgggt cagttccggc tctacttcct
     4741 cgaaaacgca agccaccaca accggggcga tggcgcggat atgatcaatc aacccttcgg
     4801 tgtcgctgct atcggccccg gtgcgggtta aatcagcctt tttgagccag aaataggcta
     4861 tctgattttg atgtgccaga cggaagtggc tgtagatatg gcgcagcaac cgggcacggg
     4921 agagcggata agactggtac acttcattac agatcctggc cagattggcg ccgcggctca
     4981 ccaattcacc tgctacgtgg tacgtgccgg gctgggtgct ggcatactga aacgagcctg
     5041 tatccgtgga taccgccgtg aacagacagt cggcgatgcg ccgagtgatg ggccatttcg
     5101 caatcttcag cagccggtaa accagttctc cggtagaggg ttcgcgcacc gaaacccagt
     5161 tgaggtcagc gaaccgtgag ttgctctcat ggtggtcaat gttgatgaag agcttgcgcc
     5221 ggctaatgta ttgcccgacc gagcccaagc gatcgaatgt ggcggcatcc accgcaacca
     5281 cgcagtcaaa ttccaatccg ggcattggtt tctggataat gccgtccgca tcaagaaatt
     5341 cgtacttttg cgggatgcgg tcttcattcc agcacactac ttgcttgccg gcgttgcgca
     5401 gcgcgagcgt caatccaagt tgcgacccga cacaatcccc atccggtcga atatgccccg
     5461 ctacacaaat tgtccggctt tcctgaaggc ctgcgatgat gcgatcaata atcctggggt
     5521 gtggtctcat ttagtgaaaa caacctcagc actgcagcaa tttccgtttc gaacggttat
     5581 tccgggggct cctccggaag ggtcttttcc aattcctcga tgatctccag cacacggttc
     5641 ccgcgcgcga tagaatcgtc caccagaaag cgcagccgcg gtgtgtactt caaaataacc
     5701 gctcctgcca ctaattcttg aatacgccgg cgatgttgtc gcagcaggct taatccctgg
     5761 cgctgctggt cggcactgcc caggatgctg atgaagacaa acgccgactt caagtcacca
     5821 gccacctcca catcgttgac cgtgatcaaa cccgcctcgc tgacttggaa ttcccggcga
     5881 atgacctcgc cgatctcccg cttcagcaat tcgcgcacgc gttggagcct gagagaaacc
     5941 atgtctgatc gatccgttag gaggtaatgg caaaccttcg ctggccgcaa gacgggcaga
     6001 cattgctaac ccaattagcc cggcaattca gcgcctccgc tgctacgatt ttcacaactt
     6061 ctgggcgatt ttttccatcg tgtagcactc gatgctatcg ccaatctgaa attcacggaa
     6121 cccttcgatc cgaatgccac attccatacc ggcacgcacc tcgttgacct cgtcctgaaa
     6181 ccgacgcagc gactggacga ttccttcata aatgatctct ttgcgccgtc gcacccgaac
     6241 tttccccttg gcaatgcggc cgctaacaac catgcacccg gccaccgggg cgcctttgga
     6301 cagttcaaaa atcttgcgca cttccgctgc gccgaccgtg atatccttca ccagcggttc
     6361 cagcaatccg gccatcgctt cttttacctg gtcaatcagc tcataaataa tggcatattg
     6421 cttgatctgc acgccttcgt gcttggcccg gtcagccacg ccactgtcaa tgcgcgcgtg
     6481 aaaacccaga attacagcat tcgaagctga ggccagcgcc acatcagatt cggtgatggt
     6541 gccgacggcg ctatgaatga cttccagcga taccttgtcc gattcgatct ttttgagggc
     6601 atccacgatg gcctcgaccg agccttgcgt gtcagccttg accaccacct tgagttcctt
     6661 gcccgaactc gaatcgagac ggtcaaagag attctccaaa gtgaccttgg cgcgcgcagg
     6721 ttcctgcccc agcgcccggg cttccaaaat acgctgctcg gcaagttcgc gcgccgcttt
     6781 ctcattctct actacattaa actccaaccc cgcttccggc acgccattca agcccaggat
     6841 cttcaccgcc accgaaggcg cggcttcttt caggcgcttg ctctcttcgt tgatcaacgc
     6901 tcgcacgcgg ccatagtatt gcccgcaaag caccacatcg ctgacgcgca aggtgccttt
     6961 gcgcaccagc acggtagccg taggtcctcc cggctccaga cccgattcaa tcacattccc
     7021 cttagcgcgc cgattgggat tggccttgag ttccagcaga tcggcttgga gcaggatcat
     7081 ttcgatcaac ttgtccacgc cctgttttgt cagcgcggaa acatccacaa aaatagtgtc
     7141 gccaccccaa tcgtccggaa ccagcccttt atcctgcaat tgctgacgaa ccctcaaggc
     7201 gttggcgttg gggtggtcta ttttattcac tgcgaccagc accggcactt tagctgcctg
     7261 cgcgtggctg agcgcctcta gggtttgcgg cattactccg tcgttggcag ctacgaccag
     7321 cacaaccacg tcagtcacat tcgccccacg agcccgcatt gaactaaagg cagcatgccc
     7381 cggagtatcc aggaatgtaa cttgggccag tgccttcttg tggtcggggt gcgggaagga
     7441 aatcgtataa gcaccaatgt gctgcgttat gccgcccgct tcgctggcga caacattggc
     7501 cttgcgaatt acatcaagca aggtggtctt gccgtggtca acgtggccca tgatggtcac
     7561 caccggaggc cgcggaatca aatcctcggc tttatcttcc ggatcctgat caatcgtggg
     7621 gccaataggt ttatgaagcc caccgtcgcg cacgcgcctt tccgcttcaa accggtagcc
     7681 atacttggcg caaatcctct gcgcctcttt ctcgtcaata gcctggttga cgctggctaa
     7741 cacgcccaat tccatcaagt ccgcaatgat cttgaacggc ctctgtttaa gctgttccgc
     7801 cagatctcgc actacgatcg gcggcttgat agtaataacc tgggcatcga gcggcagttg
     7861 caacttggct tcggcactgg ctttcgccgc caccgcccct ttcgtaaccc ctactccttt
     7921 gccccccgtc gtccccgaag ccaccgtgcc ggttcgtcca ccagagccgc cttcgcgtgg
     7981 aaaaaagtcg gccttcttcc ctggacttgc ccctcgaggc ggcgcttttt gtgcacctga
     8041 tctttcgctc cctttggggg gcggcttcgg ccgcaactga atgaatccaa ccttgtcgcc
     8101 caccttgaaa accggcggcg gtggcggctc taccacggct gattccaatt gtggttgagg
     8161 ttgaacctcc gccacaactt gcggcgcgag cgagggttcg cttggcacgt ccgccacctc
     8221 cgcttccgga gctggaatag cgggaagttc ctctatcacc ggcggaacca ccgcttccgc
     8281 tacttcgggc tccacgctcg gcttgatctc gattaccggt ggcgcagcgg cagtttctgt
     8341 cggctgttca accgggattg gtgaggtttc ttgcgccgaa accggagccg gttcggcaag
     8401 ctccggatgc tctttgagaa tctccagttc gaggtattca gccgttatct tgtccaatga
     8461 actggaagct acccgcgcag tggtaatgcc cagagacttc gccttggcga gaacttgctt
     8521 gctctcccag ccgagtttct ttgcaatttc gtatatccga acgggcatat ttctctatca
     8581 cctcaaaccc aggttgctat gcagctttca cggggctcga tcccaaatcg taagactttc
     8641 tgacaatacc agtataatca actcgaatta attggaaatt ggagtttcac caactttgag
     8701 cgtacgtcgg gccgcctcag cacgcgcggc ttccaaaata gccgccccct gatcgcccaa
     8761 acccgggatt tccgccaagt cactttcatc ggcctgcaac aaatcctcca gccgtgtcaa
     8821 tccatgatgc accagcacat ccgcctgctc ccgcgtaatc ccggtaatcg ccgccaggga
     8881 atccaccgct tcggccactt tttcctcgaa ccctttggtc accactactt ccggctcgat
     8941 gtccacctgc caacccgtca gcttcgaggt caaccgcgcg ttctgacccc gcttgccaat
     9001 ggccagggaa agttggtccg cgctaaccgt aattttgact cgcttgtggg cctcgtctat
     9061 ctcgaaggcc tttaacttgg ccggcgccag tgcgttagtg ataaagttct ttatattcgc
     9121 atcccagcga ataatatcca ccttctcgtt attgagttca cgcacgatgt tcttgacgcg
     9181 ctgcccgcgc aatccaacac aggcgcctac cggatctact ttctcgtcac gcgtccacac
     9241 cgccagcttg gtccggaaac ccggctcacg agcaattccc ttgatttcaa tggtgccatc
     9301 attgatctcc gaaacctcaa tctgaaacag cttgacgaca aagcgcggat cggaacgaga
     9361 caggataatc tcggggccat gcgggccttc ttccaccgct ttgacataac agcgaatccg
     9421 ctctcccagt tgatattctt cggtcggcac gcgctcccgg ttgggcagca acgcttcgta
     9481 tttccccaaa tccacaaaca cgtcggaccg atcgaaacgc cgaaccgccc cgctaacaac
     9541 gtcgcccacg cggtctttga actcggtaaa aatcagtgct ttttccgcgc gccgaatgtg
     9601 ctgcatcagc gcctgccgcg catactgggc ggcgatgcgg ccaaaaccca caggcgtcac
     9661 ttccacctcc aactcatctc ccacttgcgc gtccgctttt atccgtcggg cgtcgaaaag
     9721 cgcaatctgg ccatgcctgg aaatgactct ttctgctacc tgcagcttcg caaacgcgcg
     9781 tatatccccg ctcttctggt ctatatccac acgcaattcc cgcgccgggc caaccgcttt
     9841 cttcgcggcg gacaacagag cttcctggac cgcagcggtc aggacctctc gactgaggcc
     9901 cttctcgcgc tcccagaatt ccaagactgc taaaaagtcg gcgttcataa attttcaacg
     9961 gcaacccctc acgggggaat aaaaaagtcg gtagataagg ctcttccgac tttcgcgaac
    10021 tggcgaacca gcaatttgta ccgttctagc atagaagtta tgaaccgcgt ctaaaacagt
    10081 caagcctcga ttcgttgtca attcgttgta accgcatctc agtttatgaa cagctttttc
    10141 cgggcataac cttgagaaac gacagattac cctgcggctt acgtcctccc gtctgctatt
    10201 ttcaggccag tcgaactggt ggccgtgagt caacaacgct aggagcaacc ccgcaaactc
    10261 aaatcagccc cagagccacc tcctcaactt gtattaatct ttcacagaaa tagcagcgag
    10321 tgctggcgga gggcaacctg cgagagatct gcaggt
//
LOCUS       DAOPQG010000124        10273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7414_length_10273_cov_14.656978, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000124 DAOPQG010000000
VERSION     DAOPQG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7414_length_10273_cov_14.656978"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1254
                     /locus_tag="PKI07_11940"
     CDS             283..1254
                     /locus_tag="PKI07_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOC51794.1"
                     /translation="MNHLAQARKVFDLELAALQAVRAQLDETFTKAVELILATLRAHG
                     KIIVVGIGKSGHIGHKISATLTSTGSTSVVLNSVDALHGDLGIVNDGDVVLALSYSGE
                     SEELLNLLPALKRFPVKIIALTGAPKSALARHSDLVLKTRVPTEACPFNLAPTASTTA
                     MLVMGDALAIAVLQARGFKRQDFARFHPGGAIGRTMLLRVGSIMRSGPRNAVAGENLT
                     VKDALLIMTRARSGCLSVVNARGRLTGVFTDGDFRRHMTADAGLLARPLRSVMTRRPI
                     CIREDALAVEALKVFHERNIDDLIVVNARREPVGLVDSQDLPKLKLM"
     gene            1313..2080
                     /locus_tag="PKI07_11945"
     CDS             1313..2080
                     /locus_tag="PKI07_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC51795.1"
                     /translation="MPNILPPLAAALLLMAASAVAQVVTSPAPAPAQTAHQFQYTKTI
                     HATVDYLLYLPRGYDAQADRRWPLILFLHGAGERGTNIWKVATHGPLKHVTAGSDFPF
                     IVVSPQCPEQQTWSRDLVLALLDEVMARQAVDPRRVYLTGLSMGGYGTWDLGLAFPER
                     FAAIVPICGGGQMIGVILGSRDKGPALKSLGVWAFHGARDNVVPLEESQRMVDALKKA
                     GVAEVKFTIYPEAQHNSWTEAYRDPALYAWLLTHERR"
     gene            complement(2111..3076)
                     /gene="trpS"
                     /locus_tag="PKI07_11950"
     CDS             complement(2111..3076)
                     /gene="trpS"
                     /locus_tag="PKI07_11950"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOC51796.1"
                     /translation="MRILSGIQPSGTLHLGNYFGMMKPALELQERGQAFYFIADYHSM
                     TALTDPAERRRNSLDVALDFLACGLDPKKTVFFRQSDVPEVTELTWILGTVTPMGLLE
                     RCHSYKDKVAKGISFNFGLFAYPVLMAADILIYDSNIVPVGQDQKQHVEVTRDIAIKF
                     NELYGETFVIPEPQIRAETAKVPGLDGEKMSKSYGNTIEIFGDEKALRKKVMGIKMDS
                     RTPQEPKPDADQNLAIQILKLVAPAAAAREYEDRLRAGGLGYGDLKKALFEHYWNHFA
                     SARAKRAELAADPGYVQQVLADGAARARSVARKVLQRARAASGLE"
     gene            complement(3146..4492)
                     /locus_tag="PKI07_11955"
     CDS             complement(3146..4492)
                     /locus_tag="PKI07_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC51797.1"
                     /translation="MKGPRILIVDDDPGQRSLLDSFLRSQGFATIPVSSGEEALQVLR
                     AGGISMMISDVRMPGLSGLETLRRARQEHAVLPVLLVTAYADIREAVGAMRDGALNYL
                     AKPIDLDELLATVQQATGLARPAPRKFSADPPLPARVVARSPLMQAVFHDAALIAASD
                     SRILITGESGVGKEVLADVIHAWSPRAAGPLVKVNCAAIPENLLESELFGHEKGAFTG
                     AGARRIGRFEVANEGTLFLDEVADMSPPLQAKLLRVTQEGRFTRVGSNAELRTNARIL
                     AATNRNLEEEVKAGRFREDLFYRLNVVELNIPPLRERPEDILPLAAAFIAEFAGGKAR
                     WAPGVAECLARYAWPGNVRELRNAMERAALLSLGEFILPEHLPAKVRAGAEAAPPAGS
                     QDARGLAEIERAAIMQALRQHDFNRTETAKALGISRRALLYKLQRFREEGYPVDPG"
     gene            complement(4559..5695)
                     /locus_tag="PKI07_11960"
     CDS             complement(4559..5695)
                     /locus_tag="PKI07_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOC51798.1"
                     /translation="MQQTRTSQAPLYPAERLLLGPGPSPVPLRVLRALGAPTLGHLDP
                     QYIAIMDETCELLRQVFRTQNQLTFPVTGTGMAGMECLAVNLIEPGDEVIVGVNGVFG
                     TRMKEIMEGCGATVHALEAGWGDVFTLEQIGAAFERHPRARLLGLVHAETSTGAHQPL
                     DGIADLVHARGALLAVDAVTSLGGHDLRVDEWGIDACYSGSQKCLNCPPGLAPVTFGE
                     RALARMDARKTRVQSWYLDVSQLRKYYVGGGPSRFYHHTAPINMTYALREALLIVMEE
                     GLESRYARHQQMHQRLRRGLEALGLSYVPKHSLYTLNCIHIPAGADDAAVRRRLLEEY
                     NIEIGAGLGPMAGRAWRIGLMGHGATVRNVDLILAALRQVLGRG"
     gene            complement(6037..6906)
                     /locus_tag="PKI07_11965"
     CDS             complement(6037..6906)
                     /locus_tag="PKI07_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC51799.1"
                     /translation="MADAPAGAARPAAAAASSQPAADPLKEQVRSFWNRESCDTDQAQ
                     AEKFSRAYFEQIEQWRYGDQPFIHSFAQFTRYHGQRVLEVGFGAGTDFIQWLRAGARV
                     SGIDLTEEALANVRHRIAVYGLPPPESLQVADAENLPFPSNTFDLGYSWGVLHHTPDT
                     RKALAELVRVVRPGGEIKVMLYNRRGLYAWKMWVKHALLKGRPWKSLRWVLANHVESP
                     GTQGFTDRELRRMLAPLGLTDIRMERFITNNDRVVRRRFPYWLGGLAADTLLALTGRR
                     LGWFRGLTARKSG"
     gene            7135..7995
                     /locus_tag="PKI07_11970"
     CDS             7135..7995
                     /locus_tag="PKI07_11970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC51800.1"
                     /translation="MAEAPKISVVTPCLNSVGTIGATIQSVLDQDYTNWEHIVVDGGS
                     TDGTVAVLQQYPRLNWVSEKDEGHYHAMNKGLDRATGDAVVILNADDCFRAGALRKVA
                     TAFQQHPDWDALFGDVVFVDGQGREIYRREEAVYDFKVLLYGVDYICHQTLFVRRATY
                     ARLGGYRHKDFRNATDYEFKLRLGHAGCRVGHVPALLVDYRYHTQGQSADRRIIRNMI
                     QEAAVIRREYGHPGGWRSAPLRILFKAKRQLQKLWFRRKCDLIPGTWRLKPHLQAETQ
                     FSSNAGLDKL"
     gene            complement(8112..9269)
                     /locus_tag="PKI07_11975"
     CDS             complement(8112..9269)
                     /locus_tag="PKI07_11975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC51801.1"
                     /translation="MAGDWAADDGVEAGPGSEGMKRRRFNWVLSDIIRAGLKPYLEWK
                     AWLRGERFHCRALAGESEYNICINCDLTVSCSCQDYDGSGHIGDLNRNTFREVFFGPV
                     AQRFREQLAKGKLPIKTCTRCGDLERRPKSAPIPAPRLPYRGLMLENTVRCNLDCIGC
                     DRQSAARIRTTPQMDLNRLSRMADLVHELGIQTLCYLNLGEPFLSPNIGPELEVLRRK
                     NPDCNIWISTNGIVLNTDARRAAALHASRIYFSIAGVNDEMLVKYERRGSFQKAYANM
                     KALVEYRNARGLSQPLLEWKYLLFNWNDHPRTIERAIELARAAGVDFISFWPTHNPFY
                     GWSYRYALGRLNHIGIGNWKGRELDLRPPSAPAAAGNSAAAEAPALARETR"
     gene            9578..>10273
                     /locus_tag="PKI07_11980"
     CDS             9578..>10273
                     /locus_tag="PKI07_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOC51802.1"
                     /translation="MRIWASYVAMVLAGLISFLFFRFPFNFAQVRGRHNIPRRGKGVL
                     FVSNHITMYDSFLIGMAAYFPQNILYPSLPFMNFAARENYFRTWFSRTLFALLRTVPV
                     ERRDHPWLMRKYTEFLKRHNLLIFYQGTRSADLTVIKNGPAYAIANTPSPITVIPVYH
                     HGIERIFSRGGPKTRGLWRWLPRSLFRRPTVIFGQPIDFSECKRITETRARIAAINQR
                     IVASIAALQSLAAA"
BASE COUNT         1698 a         3524 c         3303 g         1748 t
ORIGIN      
        1 ccactccttg ggctggaagt ccgccccgag gtaatgcggg aaggctggcg ggcggggggt
       61 cttcgcgcaa gggcaatttt gggggattgg tggtggcagg cgtgggcaaa gcgccgcgtg
      121 ggggcacgcg gcctacaggg ggcgggaggg tgcggggggg actgtgggtt gccgggagtg
      181 cggtgctgcg agagggcatg aggtctgccg cggcgcgcgg cgagccagcg gccgcccaca
      241 atccggattg aatcccgcgg cctttccggg catgctgccc gcgtgaatca tctggcccag
      301 gcccggaagg tgttcgacct cgagctggcg gcgctgcaag ccgtccgcgc gcagcttgac
      361 gagacgttca ccaaagccgt cgagctcatc ctcgcgacct tgcgcgcgca cggcaagatc
      421 atcgtggtcg gcatcggcaa atccggccat atcggccaca agatttccgc cactctcacc
      481 agcaccggtt ccaccagcgt cgttctcaac agcgtggacg cgctgcatgg cgacctgggc
      541 attgtcaacg acggggacgt tgtcctggcg ctcagctact cgggcgagtc cgaggagttg
      601 ctgaacctgc tgccggcgct gaagcggttc ccggtgaaga ttatcgccct gaccggggcg
      661 cccaaatccg cgctggcgcg gcatagcgat ctggtgctca agacgcgggt gcccaccgaa
      721 gcctgcccgt tcaatctcgc gcccaccgcc agcaccacgg ccatgctcgt catgggcgac
      781 gcgctggcga tcgccgtcct gcaggcccgc ggcttcaaac ggcaggactt cgcgcggttc
      841 catcccggcg gcgcgattgg ccgcacgatg ctgctgcgcg tgggcagcat catgcggagc
      901 ggcccgcgca acgccgtggc cggcgaaaac cttacggtca aagacgccct gttgatcatg
      961 acgcgggcgc gctccggctg cctcagcgtg gtcaacgccc gcggccggct gacgggggtg
     1021 ttcaccgacg gcgatttccg ccgccacatg accgcggatg ccggcctcct ggcccgcccg
     1081 ctccggagcg tcatgacgcg ccgcccgatt tgcattcgcg aggacgcgct ggcggtcgag
     1141 gcgctcaagg tcttccacga gcggaacatt gacgatctga tcgtggtgaa cgcccggcgc
     1201 gagccggtgg gcctggtgga ttcgcaggat ttgccgaagc tcaaattaat gtagtcccgc
     1261 gcggcggccc gcccgcgggc gcgccgcgcc gcaaccccat gaaatcccga ttatgccaaa
     1321 catccttccg cccctcgcgg cggccctgct gctgatggcc gcctccgctg ttgcccaggt
     1381 cgtgacctcg cccgccccgg cgcccgcgca aaccgcccac cagttccagt acaccaaaac
     1441 gatccacgcc accgttgatt acctgctgta tctgccccgg ggctacgacg cccaggcgga
     1501 ccggcgctgg cccctgatcc tgtttctgca cggcgcgggc gagcgcggca cgaatatctg
     1561 gaaagtcgcc acccacggcc cgctcaagca cgtgacggcc gggtcggatt tcccgttcat
     1621 cgtcgtttcg ccgcaatgcc ccgaacagca gacgtggtcg cgcgacctgg tgctggcgtt
     1681 gctggacgag gtgatggccc ggcaggcggt ggatccgcgc cgggtttatc tcaccggcct
     1741 gagcatgggc ggctatggca cctgggattt ggggctggcc ttcccggaac ggtttgccgc
     1801 catcgtgccc atttgcggcg gcggccagat gatcggcgtg atcctgggca gccgggataa
     1861 aggcccggcg ctcaaatccc tgggcgtgtg ggccttccac ggcgcccggg ataacgtggt
     1921 gccccttgag gaatcccagc gcatggtgga cgccctgaaa aaggccgggg tggccgaggt
     1981 gaagttcacc atctatcccg aagcccagca taactcctgg acggaggctt atcgggatcc
     2041 ggcgctttat gcgtggctgc tcacgcatga acggcggtag ggggcggcgc ccggccgccg
     2101 ggcgggaggc ttactccagc ccgctggcgg cgcgcgcccg ctgcaaaacc ttccgcgcga
     2161 cgctgcgcgc ccgggccgct ccgtccgcga gcacctgctg gacatagccc gggtcggcgg
     2221 ccagttccgc ccgtttggcg cgggcggagg caaagtggtt ccaatagtgc tcgaacagcg
     2281 ccttcttcag gtcgccatag cccagcccgc ccgcgcgcag ccggtcctcg tactcccggg
     2341 ccgccgccgc cggggcgacc agtttcagga tttgaatcgc caggttctgg tcggcatccg
     2401 gtttgggctc ctgcggcgtg cggctgtcca tcttgatgcc catcactttc ttgcggagcg
     2461 ccttttcgtc gccgaaaatc tcgatggtgt tgccatagct cttgctcatt ttttccccgt
     2521 ccagccccgg caccttcgcc gtctcggcgc gaatctgcgg ctcggggatg acaaatgttt
     2581 ccccgtataa ctcgttgaac ttgatggcga tgtcgcgcgt gacctcgacg tgctgcttct
     2641 ggtcctggcc cacggggacg atgttcgagt cgtagatcag gatatccgcc gccatcagca
     2701 ccgggtaggc gaacaggccg aagttgaagg aaatgccctt ggcgaccttg tccttgtagg
     2761 aatggcagcg ctccagcagg cccatcggcg tgactgttcc cagaatccac gtcagctcgg
     2821 tcacctccgg cacgtccgat tgccggaaga acacggtctt tttcgggtca agcccgcagg
     2881 cgaggaaatc cagcgccacg tcgagggagt tgcggcggcg ctcggccggg tccgtcagcg
     2941 ccgtcatgga atggtaatcc gcaatgaaat agaacgcctg cccccgctcc tgcagctcga
     3001 gcgcgggttt catcatccca aagtaattgc ccaaatgcag tgtgcccgaa ggctgtatgc
     3061 ccgataaaat acgcatcccc gcagcttaaa ccgggacttg ccggccggga aagaaggaaa
     3121 aaagcgcgcc cgcgcgcgcg cggcgtcaac ccgggtccac cgggtagccc tcctcgcgga
     3181 agcgctggag cttgtagagg agggcgcggc ggctgatgcc cagggctttg gcggtctcgg
     3241 tgcggttgaa atcgtgctgg cgcagcgcct gcatgatggc cgcccgctcg atctccgcca
     3301 ggccgcgggc gtcctggctc ccggcgggcg gcgccgcctc cgcgcccgcg cggaccttgg
     3361 cgggcaggtg ctccggcagg atgaattcgc ccagggacaa caacgccgcg cgctccattg
     3421 cgttgcgcag ctcgcggacg ttgccgggcc aggcgtagcg cgccaggcac tcggcgacgc
     3481 cgggcgccca ccgcgccttg cccccggcga actccgcgat aaaggccgcg gccagcggca
     3541 ggatgtcctc gggccgctcg cgcagggggg ggatgtttaa ctcgaccacg ttgagccggt
     3601 agaagaggtc ttcgcggaaa cgcccggcct tgacttcctc ttccaggttg cggttggtgg
     3661 cggcgaggat gcgggcgttg gtgcgcagtt cggcgttgga gccgacgcgg gtgaagcgcc
     3721 cctcctgggt gacgcgcagc agcttggcct gcagcggcgg ggacatgtcg gccacttcat
     3781 ccaggaacag cgtgccctca ttggccacct cgaagcggcc gatccgccgg gcgcccgccc
     3841 cggtgaacgc ccctttctca tgcccgaaca attcgctctc cagcaggttc tccgggatgg
     3901 ccgcgcagtt gaccttgacc agcgggccgg ccgcgcgggg gctccaggca tgaatgacat
     3961 cggccagcac ctccttgccc acgccgctct cgcccgtaat caggatgcgg ctgtccgagg
     4021 cggcgatcag cgccgcgtca tggaacaccg cctgcatgag cgggctccgg gccaccaccc
     4081 gcgccggcag gggcgggtcc gcgctgaact tccgcggcgc gggccgggcc aggcccgtcg
     4141 cctgctggac cgtggccagc aactcgtcca ggtcaatcgg tttggccagg taattcagcg
     4201 cgccgtcgcg catggcaccg accgcctcgc gaatatcggc ataagcggtg acgagcagga
     4261 cgggcaggac ggcatgttcc tggcgcgcgc gccgcagggt ttccaggccc gaaagccccg
     4321 gcatgcggac atcggagatc atcatgctga tgccccccgc ccgcaggact tgcagcgctt
     4381 cctcgcccga gctgaccggg atcgtcgcaa agccctggct gcgcagaaac gaatccagca
     4441 ggctgcgctg ccccgggtca tcatccacaa tcagaatgcg cgggcctttc acgttgtttg
     4501 cgcgcggttt aatgggacgc gccctggcac ggcggcggcg ccctcccggc ggcggtcgtc
     4561 agccccgccc cagcacctgc cgcagggcgg ccaggatgag gtccacgttg cgcaccgtgg
     4621 cgccgtggcc catcaggccg atccgccagg cccggccggc cattgggccc agcccggcgc
     4681 caatctcgat gttatattcc tccagcagac ggcgtcggac ggcggcatcg tccgcgcccg
     4741 ccgggatgtg aatgcaattc agggtataaa gagaatgttt gggcacgtaa ctgaggccca
     4801 gggcttccag gccccggcgc aggcgctggt gcatctgttg gtggcgcgcg tagcggcttt
     4861 ccagcccttc ctccatgacg atcagcagcg cctcgcgcag cgcgtaggtc atgttgatgg
     4921 gcgcggtgtg atgatagaag cggctggggc cgccccccac gtagtatttc cgcaactggg
     4981 agacgtccag ataccaggat tgcacccggg ttttgcgcgc gtccatgcgc gcgagcgcgc
     5041 gctcgccaaa cgtcaccggc gccagtcccg gcgggcagtt caggcatttc tgcgacccgc
     5101 tgtagcaagc gtctatgccc cactcgtcca cgcgcaaatc atgaccgccg aggctggtca
     5161 ccgcgtccac cgccagcaat gcgccgcggg cgtgaaccag gtcggcaatg ccgtccagcg
     5221 gttgatgggc gccggtgctg gtttcggcat gaacgaggcc aagcaaccgg gcgcgcgggt
     5281 gccgctcgaa ggccgcgcca atctgctcca gggtaaacac gtcgccccag ccggcttcca
     5341 gggcatggac ggttgcgccg cagccctcca tgatctcttt catccgggtg ccaaaaacgc
     5401 cgttgacgcc gacgatgacc tcgtcgcccg gctcaatcaa gttcacggcg aggcactcca
     5461 tgccggccat acccgtgccg gtaaccggga aggtaagctg attttgggtg cgaaaaacct
     5521 gccgcagcag ctcgcaggtc tcgtccatga tggcgatata ctgcgggtcc aggtggccca
     5581 gggtcggcgc ccccagcgcg cgcaaaacac gaaggggcac gggactgggg cccggcccca
     5641 ggagaagacg ttcggcgggg tagagtggag cctgtgacgt tcgggtctgt tgcataccct
     5701 tggttagtgg cagcaccgcg ccaaagcaag ccgatttatc tgcctgaggg ggaaaggccc
     5761 gggacatcgt gaccgcgggc ttttcccggc acgcaataag cgaccgttgc cgctgccgcc
     5821 gcgatgcgga tgccagccgt agcgagtcgg attccctccc atccaatccc gtgtcatccc
     5881 agcaagtgac cccttgaacc ccccggaaaa agtggttccc gcgacagagc gtgaagctcc
     5941 atgaaacaag gttgccggca aacggaaagc cggcaccgcc ggtgtggggc gggcatcggc
     6001 cggatgctgc cgggcgcgcg gggaggggtt cccgcttcag ccggattttc gtgcggtaag
     6061 gccgcggaac cagcccaggc ggcggccggt cagcgcgagg agcgtgtcgg ctgccagccc
     6121 gcccagccag taaggaaagc ggcggcgcac cacccggtcg ttgttggtga taaaacgctc
     6181 catgcgaata tccgtcaggc ccagcggcgc gagcatccgg cgcagttccc ggtctgtgaa
     6241 gccctgggtg cccgggcttt cgacatggtt cgccagcacc cagcgcaggc ttttccaggg
     6301 gcggcctttg agcaacgcat gcttcaccca catcttccac gcgtagaggc cgcggcggtt
     6361 atagagcatg accttgattt ccccgcccgg gcgcaccacg cgcaccaatt cggccagcgc
     6421 tttccgcgta tccggcgtat ggtgcagcac gccccacgaa tagcccaggt caaacgtgtt
     6481 cgaggggaag ggcaggttct cggcatcggc cacctgcagg ctctccggcg gcggcaggcc
     6541 atacaccgcg atgcgatggc ggacgttggc cagcgcctct tcggtcaggt ctatccccga
     6601 cacccgcgcc ccggcccgca gccactggat gaaatccgtg cccgcgccaa accccacttc
     6661 cagcacccgc tggccgtgat agcgggtgaa ctgggcaaag gaatgaataa acggctgatc
     6721 cccgtagcgc cactgctcga tctgctcaaa atacgcgcgg ctgaactttt cggcctgcgc
     6781 ctggtcggtg tcacagctct cccggttcca gaagcttcgg acctgctctt tcaaggggtc
     6841 ggcggcgggc tgggaggatg ccgccgccgc cgccgggcgc gcggcgccgg ccggagcgtc
     6901 agccatattc cgggttgagt ccatatcagc ggtctgcgcg gggcatccta accatcgcct
     6961 ttccgccggg caaccagttt tgccccgctc ttccaggagc cgccgccgcc acgagcgcgg
     7021 agcagcgtcc cggcgccggc tccagccgcg gccgttgcga cccggagttg agattgcggg
     7081 caggcggata atgctggtac actgccgccg catcgcaaca tgacacactc cgttatggct
     7141 gaagcgccga agatttcggt cgtcacccca tgcctgaaca gcgtgggcac gatcggcgcg
     7201 acgatccaaa gtgtcctgga ccaggattac acgaactggg agcatattgt cgtggatggc
     7261 ggttcgactg acggaacggt ggcggtgctg cagcagtatc cgcgcctgaa ctgggtatcc
     7321 gaaaaggatg aggggcatta tcacgccatg aacaagggcc tggaccgggc gacgggcgac
     7381 gcggtggtga ttctcaacgc ggatgactgc tttcgggccg gcgccctgcg aaaagtcgcc
     7441 accgccttcc agcagcatcc ggattgggat gcccttttcg gggacgtggt gtttgtggac
     7501 ggccagggcc gcgagatcta ccggcgcgaa gaggccgttt atgacttcaa ggttctgctc
     7561 tacggggtgg actacatctg ccaccagacg ctctttgtgc ggcgcgcgac ttatgcccgg
     7621 ctgggagggt atcggcataa agatttccgg aacgccaccg attacgagtt caagctccgc
     7681 cttggccatg ccggctgccg ggtggggcac gtgccggcct tgctggtgga ctatcgttac
     7741 cacacccagg gccagtccgc cgatcggcgc attattcgca atatgatcca ggaagccgcc
     7801 gtgatccgcc gggaatacgg ccaccccggc ggctggcgca gcgcccccct gcgaatcctt
     7861 ttcaaagcca agcgccaact gcaaaaactc tggttccgcc gcaagtgcga cctcattccc
     7921 ggcacctggc ggcttaagcc ccacctgcag gccgaaaccc agttctcctc caacgccggc
     7981 ctggacaaac tctagcccgg gcagccccgg tcggcgggac cagcgcatgg gccccgggcc
     8041 tgcggacggg gtcgccaggg catcggcggg ctgcggcggg ccgtgcgcgc gccgccaccc
     8101 ttcaaggtgc gtcagcgggt ttcgcgggca agggccggcg cctccgcggc ggcgctgttc
     8161 ccggcggcag ccggggcgct gggcgggcgc aaatccagct cgcggccttt ccagttgccg
     8221 atgccgatgt ggttcagccg gcccagggca tagcggtagg accagccgta aaacggatta
     8281 tgcgtgggcc agaaggagat gaaatccacc ccggcggccc gggccagctc gatggcccgc
     8341 tcgatggtgc gggggtggtc attccagtta aacagcaggt atttccattc cagcagcggc
     8401 tgggaaaggc cgcgcgcgtt gcggtattcc accagcgcct tcatgttggc gtaagccttc
     8461 tggaaactgc cgcgccgctc atacttcacc agcatctcgt cgttcacacc ggcgatcgag
     8521 aaatagatgc ggctggcgtg cagcgctgcc gcccgccggg cgtcggtatt cagcacgatc
     8581 ccgttggtcg aaatccaaat gttgcaatcc gggttcttgc gccgcagcac ctccagctcc
     8641 ggcccgatgt tcggcgagag aaacggctcg cccaggttca ggtagcacag ggtttgaatg
     8701 cccagctcgt ggaccaggtc cgccatgcgc gacagccggt tcaaatccat ctgcggcgtc
     8761 gtgcgaatgc gcgccgccga ttgccggtcg cagccgatgc agtccaggtt gcagcgcacc
     8821 gtgttttcca gcatcaaccc ccgatacggc agccgcggcg cggggattgg cgcggatttg
     8881 ggccgccgct ccaggtcccc gcagcgcgtg caggtcttga tcggcagctt tcctttggcc
     8941 aattgctcgc ggaaccgctg ggccaccggc ccaaagaaaa cctcgcgaaa cgtgttccgg
     9001 ttcaagtccc cgatatggcc cgagccgtca tagtcctggc aactgcacga caccgtcagg
     9061 tcgcagttga tgcaaatgtt atattccgac tccccggcca gcgcgcggca atgaaaccgc
     9121 tccccgcgca gccacgcctt ccattccagg taaggcttca gccccgcgcg aatgatgtcg
     9181 ctcagcaccc agttaaaccg ccgccgtttc atgccctcac tcccggggcc ggcctcgacg
     9241 ccatcgtccg ccgcccaatc gccagccacg cccgccgcaa aaatttcatg caatcaccct
     9301 atcacacccc cacccctgga gccagccgaa acggcccggc agcccggttg ccgggggcgc
     9361 cggttcgggt gcgccggccc gccgcccggc ccggccgggc cgcctcccgg gcgcggccct
     9421 aggcgcccga ttgggccttt gctttaagcc ggatttgtgc taaacaattc cggatgacga
     9481 aaaccaaaca aaacgaggcc gcgaccccgc ccgggcgggt ggccgcgcag ccaggccggg
     9541 cctcccgatg atgctgaccg ccaaaatacg ccgccgtttg cgcatctggg cctcctatgt
     9601 ggccatggtt ttggccggcc tgatctcctt tctctttttc cggttcccgt tcaactttgc
     9661 ccaggtgcgg ggccgccata acatcccccg gcgcggcaaa ggggtgctct tcgtctcgaa
     9721 ccacatcacc atgtacgatt cgttcctcat cggcatggcc gcctatttcc cgcaaaacat
     9781 tctctatccc tcgctcccct tcatgaactt cgccgcccgg gaaaactact ttcgcacctg
     9841 gttttcccgg accttgtttg cgctgttgcg caccgtcccg gtcgaacgcc gcgatcaccc
     9901 ctggctgatg cgcaaataca ccgagttcct gaaacgccac aacctcctca tcttttacca
     9961 gggcacccgc agcgccgacc tgacggtcat caaaaacggc cccgcctacg ccatcgccaa
    10021 caccccctcg ccgatcaccg tcattcccgt atatcaccac ggcatcgaac gcatcttcag
    10081 ccgcggcggc cccaaaaccc gcggcctctg gcgctggctg ccgcgcagcc tcttccgccg
    10141 ccccaccgtc atcttcggcc agccgattga tttctccgaa tgcaagcgca tcaccgaaac
    10201 ccgcgcgcgc atcgccgcca tcaatcagcg catcgtcgcc agcatcgccg ccttgcagtc
    10261 cctggccgcc gcc
//
LOCUS       DAOPQG010000125        10202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7489_length_10202_cov_14.967675, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000125 DAOPQG010000000
VERSION     DAOPQG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7489_length_10202_cov_14.967675"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..946
                     /locus_tag="PKI07_11985"
     CDS             245..946
                     /locus_tag="PKI07_11985"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC51803.1"
                     /translation="MKTSVFLVIVAVGLGATCRLPGETSRPFNGKDLTGWKARAEGNT
                     PSQWKVGRAKLSPANPRELAVTPGGHELINDTRGHGQSLDLYSTAVFGDAVITLEVMV
                     PEKSNSGIYVMGEYEVQVLDSFGKDAHPGPGDMGAIYDAQPPRNPKYRPPGEWSRYEI
                     HWRAPRFDAAGNKTAPAKFLKVILNGRVIHENLEMPKPTPGGLDGREKARGPLMFQGN
                     HGPVAYRKIRVRPLP"
     gene            943..1719
                     /locus_tag="PKI07_11990"
     CDS             943..1719
                     /locus_tag="PKI07_11990"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51804.1"
                     /translation="MSRFVNLELDGAPKDRPPPPGTPVKDEAYYCAAAQAALANGEFE
                     PALRLYGKVLECNPQNPVAWVGQVRMLIELGRFHEAGRWADLALARFPHDPELLAAKG
                     VALGRGGDLPGALAFSDSALEERGDLAYVWLARGDVLLARREARADYCFDKALLLAPR
                     DWQVAWLGARVRCYYRQFALALKLLQQAVEWNATHFLVWLELGRCQEALGLAGPARHS
                     FEQARQLNQNCPAAGLALARVGRMGIGARWRGWWRRWGNG"
     gene            1716..2777
                     /locus_tag="PKI07_11995"
     CDS             1716..2777
                     /locus_tag="PKI07_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOC51805.1"
                     /translation="MSTRTLERLFAAAAEFDASDLHLVAGVPPAYRVNGEIILAEEDA
                     LAEAALAEIAGALLNAQQRRKFDEEWELCISLRHPAAGRVRATFYRRNGYPELSFRFC
                     GDRIESREALGLPEKLDDWARKPNGLVLITGPTGAGKTTTLNYLVDLINRERRCKIVT
                     IEDPIEFVHENQRAIVVQQEVLTDVRSFNRALIHVLRQDPDVLVIGEMRDFEAIATAL
                     TAAETGHLVLATMHSPNILHALERIVGVFEGSAQRQIILQLSNALQGIIAQELLPAVD
                     RTRRVLAYEMIAANNAVRNLIRENQLHQLENVMQTGRKEGMVLMDNCLYELYCQCRIT
                     YETAISRARHPENIRQKLA"
     gene            2902..4122
                     /locus_tag="PKI07_12000"
     CDS             2902..4122
                     /locus_tag="PKI07_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51806.1"
                     /translation="MNSPVWQWVLRAAVLVVVAGGVGWWGFRCLKRSDDPARLAFKWG
                     LSALLLGVLVFWGFPAVGEGRIHPMLGVPLVAAVGLVLAIIWGRSIGLWAARPLSSLY
                     DGGQEEPAPQPVYSRAQSLQKKGRYAEAAREIWRQLERFPTDREGQMLLAQIQAEDLK
                     DLAAAELTLTRFCAQPGHAPANLAFALFSLADWHLRLAQDRAAAQRVLEQVIARLPDS
                     EFARAAAHRIAHLDHLEMRLAPHEPKKFTVVAGPTNIGLRSRGEPDPPAADDGEQEVA
                     KWVRHLTQHPLDTEARERLAILYADCCGRLDLATAELEQLIALPNLPEKQAAHWLNRL
                     ADLQIRTGAGEEAIRQTLHRIEERAPQSVHAELARQRLARLKLELKAGAATPTVKLGV
                     YEQNLGLKRDLPRR"
     gene            complement(4108..4506)
                     /locus_tag="PKI07_12005"
     CDS             complement(4108..4506)
                     /locus_tag="PKI07_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51807.1"
                     /translation="MKTKIAAGLLGALVLAAGCVRTVNDRHHFALSPGRDKFESRYER
                     TVEQVYSASTAVLRRNGTIGRESIINTTTNQVRAIEGQINNRNVFVRVEPVDPVVTSV
                     KVQVRSKGGGTDLTLTQEIQKQIAIELATR"
     gene            4645..5541
                     /gene="larE"
                     /locus_tag="PKI07_12010"
     CDS             4645..5541
                     /gene="larE"
                     /locus_tag="PKI07_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010494028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOC51808.1"
                     /translation="MVSEKYERLCGLLRSYGSCLVAYSGGVDSFFLAYVAHAMLGEKS
                     LAVIADTPSLPRRELDQALALARQYQLPLRVIQTREFQNPNYLANPNNRCFFCKHDLF
                     TELVPLARAEGLACIAYGENASDLGDYRPGAQAAAQFQARAPLKEAGLTKADIRALSA
                     RLGLPTADKPQMACLSSRIPYGEAVTPEKLRMIEQAEDFLRDLGFYDVRVRHHELRKS
                     ALLATSLHLARIEVGAGEMPKLLENGGSAKVAAALKKFGYAHVTLDLQGYRRGSANEV
                     LPLADAADAASRPPPAPRPQAA"
     gene            5685..6959
                     /locus_tag="PKI07_12015"
     CDS             5685..6959
                     /locus_tag="PKI07_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOC51809.1"
                     /translation="MPQFSYKARRRTGEVVEGVLEVADRSAALVQIERLGLFPVAVDA
                     AKGGQAHAAERADRQKRDWGALLPPALREVLTRKRKPKLQELSTFTQQLANLLNSGMP
                     LTVALNSMTHLETKGIPAEVSRNLKQDVMEGRSLSDSMAKQPRIFSDLYVNMVRAGES
                     SGALVEVLRRMADHFERFAQVQSKFSSALVYPAFVSVVGIAIMFFFMSYMLPKFLSIF
                     EDMKVPLPMMTQLLVKISHVFSRYWWLMLALIIAAVIVFKRFQATPEGRRRIDLWRMQ
                     APIFGKAIKLNIFGQFARTLSTLLENGVPVLTALKITEQIIPNTVVKEAIAKTREEVT
                     DGKTIAQPLARSKIFPQLMVDLVKIGEDTGDVPGALKNVADTYENELSIALRVMTNMI
                     EPVLIIVMAVGVGFLLLSVLSAMFAMTANIAR"
     gene            6964..7575
                     /locus_tag="PKI07_12020"
     CDS             6964..7575
                     /locus_tag="PKI07_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51810.1"
                     /translation="MSFRFPVSGPRAAPGGAGGPAPRPPRAFTLIELMIVVGIMGTVM
                     AMSAPVVYKVWRKAPMRQAVSSVVEVFSRARARAILQGTTVEVILYPRDNRLGVAGAA
                     VAARPAAEAEAHAIPNAPPAAGSGLAAQLPEDVIIETLDINMSGIEYNDAEEARIRFY
                     PNGVSDEMRMILFDGRERTGIELEITTGLANVVPNPLREWMRR"
     gene            7572..8030
                     /locus_tag="PKI07_12025"
     CDS             7572..8030
                     /locus_tag="PKI07_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51811.1"
                     /translation="MNRPPGTAPRVPAPPPPARAPRPAASRARRARAAFSLLEVMIAG
                     GIFFMAIFAILALVTSLLRNARGLRHAELDAGMVAAQIGNTNKLYEGVQSGDFGQWYP
                     GYSWEAEIHEAATNGLFQVDIVVLRRGLAKPVDAMSIFLFRPDSPKRGMR"
     gene            8027..8806
                     /locus_tag="PKI07_12030"
     CDS             8027..8806
                     /locus_tag="PKI07_12030"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HOC51812.1"
                     /translation="MSRPPIPSRAPRRPRPARPAAAPPGRRAFTLVEIMIAIGIFSFI
                     LAGIYSSWTAILRASKTGLDAAASAQRARVTVRVLEDSLGSAQSFAANLPLYYFAAEN
                     GSDAYLSFVARLSKSFPRSGKFGDLDVRRVTFSVEPAPDGGRQLVLRQNPLVMEVDRD
                     EQEHPVVLAKNVKEFEMLFWATDKNPPDWVDEWREQKTNQLPAMVMISLRLADNPRSS
                     RITEEITRIISLPAVTVQPMWQMPQGLANPPPPGAPAPNPN"
     gene            8811..9929
                     /locus_tag="PKI07_12035"
     CDS             8811..9929
                     /locus_tag="PKI07_12035"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
                     /protein_id="HOC51813.1"
                     /translation="MRTALPASRPRARAAPRPAPPGIAMVIVMISIMVLSVLAAGFAF
                     SMKVETKLARNANSEADLEWLGRSGVEYARWVLGNSLLNPQQPYDSLDQPWATGYGIL
                     GPTNNPIGEVQKTIELGRGSFTWTITDLERKFNINSPEPVLQQILPQALNLMGVSPGE
                     STALINSILDWTDRDDQTHVEGAESRYYESSDPPYFAKNGPIDDMAELLFVKGMTPEI
                     YYGLSSTNYQPSYYSQQRNRFVRGRSPVPTVAVGLTDLFTPISTGRVNVNTAPVEVLM
                     VLPGVNEIIANAIHSRAMEPSDISGLTGPFRTVDQLRSLPDVPLELVRLIQPFCDVRS
                     KTFQVQIDADVGGYKRTFFAILGRNNQRDVQILSFYWQ"
BASE COUNT         1746 a         3592 c         3187 g         1677 t
ORIGIN      
        1 ccgtccgcgg agcggccggc gcagtttttg cagatgcgcc atagcaggcc acgatggttg
       61 agggtcttgc cggagatttc gggctgctcc ttagaccctc gcagcctcac ttgacccgga
      121 gagcggcttt gggcaatacc gcatcacccg cgtgcggggc cggggcggga tgggggagtt
      181 gaattaagcc tttgccgccc gcgccaattt cagtacaagc ggaagcatag atgccgaacc
      241 gtttatgaaa accagcgtat tcctagtcat cgtggcggtg gggcttggcg cgacctgccg
      301 tttgcccgga gaaacgagcc ggccattcaa cggcaaggac ttgacgggct ggaaggcgcg
      361 cgccgagggg aacaccccga gccagtggaa ggtcgggcgc gcgaaactct ccccggccaa
      421 tccccgggag ctggctgtca cgcccggcgg ccacgaactg atcaacgaca cccgggggca
      481 tgggcaaagc ctggacctgt acagcaccgc ggtgtttggc gacgcggtca tcacgctgga
      541 ggtgatggtg ccggagaagt ccaacagcgg catttacgtc atgggcgaat acgaagtgca
      601 ggtgctggac agtttcggca aggatgctca tcccggcccc ggcgacatgg gggccatcta
      661 tgacgcgcag ccgccgagga atccgaagta tcgtccgccg ggggaatgga gccggtatga
      721 gattcattgg cgggcgcccc ggtttgatgc cgcgggcaac aagaccgccc cggcgaagtt
      781 cctcaaagtc atcctgaacg ggcgggtgat ccatgagaac ctggaaatgc cgaagccgac
      841 ccccgggggc ctggatggcc gggagaaggc gcgcgggccg ctgatgttcc agggcaatca
      901 tgggccggtc gcctaccgca agatacgcgt ccggccgctg ccgtgagccg ctttgttaat
      961 ctcgagctgg acggcgcgcc caaagaccgg cccccgccgc ccgggacgcc ggtgaaggac
     1021 gaggcctatt actgcgccgc ggcgcaggcg gccctggcga acggcgagtt cgagccggcg
     1081 ctgcggctgt atgggaaggt gctcgagtgc aacccgcaga atcccgtggc gtgggttggg
     1141 caggtgcgga tgctgatcga gttggggcgc tttcacgagg cgggacgatg ggcggatctg
     1201 gcactggcgc gctttcccca cgacccggag ttgctcgccg ccaagggcgt cgcgctgggc
     1261 cggggcgggg atttgccggg ggcgctggcg ttttcggatt cggccctcga ggagcgcgga
     1321 gacctggcgt atgtgtggct ggcgcggggg gatgtgttgc tggcgcgccg ggaggcgcgg
     1381 gcggattact gttttgacaa ggccctgctg ctggcgccgc gtgattggca ggtggcgtgg
     1441 ctgggggcgc gcgtgcgctg ctattatcgc cagttcgcgc tggcgctgaa gttgttgcag
     1501 caggcggtgg agtggaatgc cacccatttc ctggtgtggc tggagctggg gcgctgccag
     1561 gaggcgctgg ggctggccgg cccggcccgc cattccttcg agcaggcgcg gcagcttaat
     1621 caaaattgcc ccgccgccgg gctggcgctg gcccgggtgg gccggatggg aatcggggcg
     1681 cgctggcgcg gctggtggcg ccgctggggg aacggatgag cacgcgaacc ctggagcgcc
     1741 tgtttgccgc cgccgcggag ttcgacgcgt ccgacctgca cctcgtggcg ggcgtgcccc
     1801 cggcctaccg ggtgaacggc gagattatcc tcgccgagga ggatgccctg gcggaggcgg
     1861 ccctggcgga aatcgccggc gcgctgctca acgcgcagca gcggcggaaa ttcgacgagg
     1921 aatgggagtt atgcatttcg ctgcggcatc ccgccgccgg gcgggtgcgc gcgacgttct
     1981 accggcgcaa cgggtacccg gagctgagct tccgcttttg cggcgaccgc atcgagagcc
     2041 gggaggccct ggggctgcct gagaaactgg acgattgggc gcgcaagccc aacgggctgg
     2101 tgttgatcac cggccccacc ggcgcgggca aaacgaccac gctcaactac ctggtggacc
     2161 tgatcaaccg cgagcgccgg tgcaagattg tcaccatcga agaccccatc gagttcgtgc
     2221 atgagaacca gcgggcgatc gtggtgcagc aggaggtgct caccgatgtg cggtcgttca
     2281 accgggcgct gatccacgtc ctgcggcagg acccggacgt gctggtgatc ggcgagatgc
     2341 gcgatttcga ggccatcgcg acggcgctga ccgccgccga gaccgggcac ctggtgctgg
     2401 ccaccatgca ttcgcccaat atcctgcacg cgctggagcg gatcgtgggc gtgttcgagg
     2461 gcagcgcgca gcggcagatc attctgcagt tgtccaacgc cctgcagggg atcatcgcgc
     2521 aggaattgct gcccgccgtt gaccgcaccc ggcgcgtgct ggcttacgag atgatcgcgg
     2581 ccaataacgc ggtgcgcaac ctgatccgcg aaaaccagct tcaccagttg gagaatgtca
     2641 tgcagaccgg gcgcaaagag ggcatggtgc tgatggataa ctgcctctac gagctttact
     2701 gccaatgccg gatcacctac gaaaccgcca tcagccgcgc ccgccatccg gagaacatcc
     2761 gccagaagct ggcctgaagc gggcggagga agcggcgggc gtcccggttc cgttgtcccg
     2821 ccgttgcccg gggccgggtc aggcgcgggc gcgggcagtt tccgcgtgcg gtttggggcg
     2881 ctgcccggtc aaagtcatgt catgaattca ccggtctggc aatgggtgct gcgcgcggcg
     2941 gtgttggtgg tggtggccgg gggagttggc tggtggggct ttcggtgcct caaacgcagc
     3001 gacgatcccg cgcggctggc cttcaagtgg ggcctgagcg cgctgctcct tggcgtcctg
     3061 gttttttggg gattccccgc ggtgggagag ggtcggatcc atcccatgct gggcgtcccg
     3121 ctcgtcgcag cggtagggct ggtgctggcg attatttggg gacgcagcat cgggctttgg
     3181 gccgcccggc cgctgagttc gctctatgac ggcggccagg aggagccggc gccgcagccg
     3241 gtgtattccc gggcccagtc tttgcagaaa aagggccggt atgcggaggc ggcgcgcgaa
     3301 atctggcggc aactcgaacg cttccccacc gaccgggaag ggcaaatgct gctggcgcag
     3361 attcaagccg aagacctgaa ggacctggcc gccgccgagc tcaccctcac gcgcttctgc
     3421 gcccagcccg gccatgcgcc ggcgaacctt gccttcgccc tgttctcgct ggcggattgg
     3481 catcttcggc tcgcccagga ccgcgcggcg gcgcagcgcg tcctggaaca ggtcatcgcg
     3541 cggctgccgg attcggaatt cgcccgggcg gcggcgcatc ggattgccca cctggaccac
     3601 ctcgaaatgc ggctggcgcc gcacgaaccg aagaagttta ccgtggtcgc gggcccgacg
     3661 aatatcggcc tccgtagccg cggggagccc gatccgccgg ccgccgacga tggggagcaa
     3721 gaggtggcga aatgggtccg gcacctgacg cagcatccgt tggacaccga ggcgcgggag
     3781 cggctggcga ttctttacgc cgattgctgc ggccggctgg acctggccac cgccgagttg
     3841 gagcagttga tcgcgctgcc caacctgccg gagaagcagg cggcgcactg gctgaaccgg
     3901 ctggcggact tgcagattcg gaccggggcg ggcgaggagg cgatccggca aaccctccac
     3961 cggattgagg agcgcgcgcc gcaatccgtc catgccgagc ttgcccgcca gcggctggcc
     4021 cggctgaagc tcgaattgaa ggccggcgcc gcaaccccca ccgtcaagct gggggtctat
     4081 gagcaaaacc tcggcctgaa acgggattta ccgcgtcgct agctcgatgg cgatctgctt
     4141 ctggatttcc tgcgtgaggg tcaagtccgt gccgccgccc ttgctgcgca cctggacttt
     4201 gacggaagtt acgaccgggt ccaccggttc cacgcggacg aaaacattcc ggttgttgat
     4261 ctggccttcg atcgcccgga cctggttggt ggtggtgttg atgatgctct cccggccaat
     4321 ggtgccgttg cgccggagca ccgcggtcga agcgctataa acctgctcca ccgtccgctc
     4381 gtagcggctt tcgaatttgt cccggcccgg cgagagcgca aaatgatggc ggtcattgac
     4441 cgttcgtacg cagcccgccg ccagcaccag cgccccgagt aaacctgctg ctatcttcgt
     4501 tttcatggcc cccatgcaat cacatttacc gaaaccgtca agcccggatg acgctttttc
     4561 ggctccccgg cggacgcccg ggtttccccg ccgcccccgc ggccgcccct cgacaacccc
     4621 cgcgcggcgt gcgagtttga ggacgtggtt tctgaaaaat acgaacggtt gtgcgggttg
     4681 ctgcgctcct atggctcctg cctggtcgcc tactcgggcg gggtggactc gtttttcctg
     4741 gcctatgtcg cgcacgcgat gctgggcgag aagtcgctgg cggtcatcgc cgacaccccc
     4801 agtctgcccc ggcgcgagtt ggaccaggcc ctggccctgg cccggcaata ccagttgccg
     4861 ctccgggtca tccagacccg cgaattccaa aaccccaact acctggccaa cccgaacaac
     4921 cgctgcttct tctgcaagca cgacctgttc accgagctgg tccccctggc gcgggcggag
     4981 gggctggcct gcattgccta cggggaaaac gccagcgacc tcggcgatta ccgccccggc
     5041 gcccaggccg ccgcgcaatt ccaggcgcgc gcccccctca aggaagccgg cctgaccaag
     5101 gcggacattc gcgcgctctc ggcccggctc ggcctgccca ccgccgacaa accccagatg
     5161 gcctgcctca gctcccggat tccctatggc gaggcggtca ccccggagaa gctccggatg
     5221 atcgagcagg ccgaagactt cctgcgcgac ctggggttct acgatgtccg cgtgcgccat
     5281 cacgagctgc ggaaatccgc cttgctggcc acctccctcc atttggcccg catcgaggtc
     5341 ggcgccggcg aaatgcccaa actgctggag aatggcgggt cggcaaaagt agcggcggcg
     5401 ctcaaaaagt ttggctatgc gcacgtcacc ctcgatctcc agggctaccg ccggggcagc
     5461 gccaacgaag tcctgccgct tgccgacgcc gcggacgcgg cgtcccgccc gccgcccgcg
     5521 ccgcgcccgc aagcggcgtg acccgatcat tttccccgcg gcccgcccgc cgcgccgccg
     5581 ctcccgcccc gcgggccggg ccgttcgcgc cggcccaaat tgctgcttgg aaaaccgcaa
     5641 cttttggcta cactgtgggt taaagaaaga agcagtcccg ctttatgcct caattctcat
     5701 ataaagcgcg ccgccggacg ggtgaagtcg tcgagggcgt gctggaggtt gccgatcgct
     5761 cggcggccct ggtgcaaatt gaacgcctgg ggctgttccc ggtggcggtg gatgcggcca
     5821 aaggcgggca ggcccacgcc gccgagcgcg ccgaccgcca gaagcgggac tggggcgccc
     5881 tgctgccgcc ggcgttgcgg gaggtgctga cccgcaaacg caagcccaaa ctgcaggagc
     5941 tcagcacctt tacccagcag ctggccaacc tgctcaactc cggcatgccg ctgaccgtcg
     6001 cgctcaacag catgacgcac ctggaaacca agggcatccc cgccgaagtc agccggaacc
     6061 tcaagcagga tgtcatggaa ggccgcagcc tctccgactc catggccaag cagccgcgca
     6121 tcttctccga cctgtacgtc aacatggtgc gcgcgggcga atccagcggc gccctggtcg
     6181 aagtcctccg ccgcatggcc gaccacttcg agcggttcgc gcaggtgcag tccaagttct
     6241 cctccgcgct ggtctatccg gccttcgtct ccgtggtggg catcgccatc atgttcttct
     6301 tcatgagcta catgctgccc aagttcctct ccatcttcga ggacatgaaa gtcccgctgc
     6361 cgatgatgac ccagctcctg gtcaagatca gccatgtgtt ctcccgctac tggtggctca
     6421 tgctcgcgct gatcatcgcc gccgtcatcg tgttcaaacg cttccaggcc actcccgaag
     6481 gccgccgccg gattgacctg tggcgcatgc aggcccccat cttcggcaaa gccatcaagc
     6541 tcaacatctt cggccagttc gcccgcacgc tctccaccct cctcgaaaac ggcgtgcctg
     6601 tgctcacggc gctcaagatc accgagcaaa tcatccccaa caccgtcgtc aaggaagcca
     6661 tcgccaaaac ccgcgaagag gtcaccgatg gcaaaaccat cgcccagccg ctcgcccgca
     6721 gcaaaatctt cccccagctc atggtggacc tcgtcaagat cggcgaggac accggcgacg
     6781 tgccgggcgc gctcaaaaac gtcgccgaca cctacgaaaa cgagctgagc atcgccctgc
     6841 gcgtcatgac caacatgatc gagccggtgc tgattatcgt catggccgtt ggcgtcgggt
     6901 tcctcctgct cagcgtgctc tcggccatgt tcgccatgac ggccaacatc gcgcgctgag
     6961 aagatgagct tccggtttcc agtttccggg ccccgggcgg ctcccggcgg agcgggcggc
     7021 cccgccccgc gcccgccccg cgcctttacg ctgatcgagc tcatgatcgt cgtgggcatc
     7081 atgggcaccg tcatggcgat gagcgcgccc gtggtttaca aggtttggcg caaagcgccc
     7141 atgcgccagg ccgtgagcag cgtggtcgag gtcttcagcc gcgcccgcgc ccgcgccatc
     7201 ctgcagggaa ccaccgtgga agtcattctc tacccccggg acaaccgcct gggcgtcgcc
     7261 ggcgccgccg tcgccgcgcg ccccgccgcc gaggccgaag cccacgccat ccccaacgcg
     7321 ccgcccgccg ccggcagcgg cctggccgcc cagctccccg aggacgtcat catcgaaacc
     7381 ctggacatca acatgtccgg catcgagtac aacgacgccg aggaagcccg catccggttt
     7441 tatcccaacg gcgtctccga cgagatgcgc atgatcctgt tcgacggccg cgagcgcacc
     7501 ggcatcgaac tggagatcac cacgggcctg gccaacgtcg tgcccaaccc cctgcgggaa
     7561 tggatgcgcc gatgaaccgc cccccgggaa ccgccccgcg cgttccggcg ccgcccccgc
     7621 ccgcccgcgc cccgcgcccc gccgcctccc gcgcccggcg cgcccgcgcc gccttctcgt
     7681 tgctggaggt catgatcgcc ggcggcatct tcttcatggc catcttcgcc atcctggcgc
     7741 tggtgaccag cctgttgcgc aacgcgcgcg gcctgcgcca cgccgagctg gatgccggca
     7801 tggtcgccgc ccagatcggc aacaccaaca aactctacga aggcgtgcaa tccggcgact
     7861 ttggccaatg gtatcccggc tactcctggg aggccgaaat ccacgaagcg gccaccaacg
     7921 gcttgttcca ggtggacatc gtggtgctcc gccgcggcct ggcgaagccg gtggatgcga
     7981 tgagcatctt cctgttccgc cccgactcgc ccaaacgggg gatgcgatga gccggccccc
     8041 aatcccctcc cgcgcgcccc gccgcccgcg ccccgcgcgc cccgccgccg ccccgcccgg
     8101 ccgccgggcc tttacgctcg tcgagatcat gatcgccatc gggatcttct ccttcatcct
     8161 cgccgggatc tattcgagct ggaccgccat cctccgcgcc tccaagaccg gcctcgacgc
     8221 cgccgcctcc gcccagcgcg cccgcgtcac cgtccgcgtc ctggaagact cgctcggctc
     8281 ggcgcaatcc ttcgccgcca acctccccct ctactacttc gccgccgaaa acggcagcga
     8341 cgcctacctg agctttgtgg cccggctctc caaatccttc ccccgcagcg gcaaatttgg
     8401 cgacctggac gtccgccgcg tgacgttctc ggtcgagccc gccccggacg gcggccgcca
     8461 actggtgttg cgccaaaacc cgctggtcat ggaagtggac cgcgacgagc aggaacatcc
     8521 cgtggtgctg gccaaaaacg tcaaagaatt cgaaatgctg ttctgggcca ccgataaaaa
     8581 cccgcccgac tgggtggacg agtggcggga acaaaaaacc aaccagttgc ccgcgatggt
     8641 catgatctcc ctccggctgg ccgacaaccc ccgctcgtcg cgcatcaccg aggaaatcac
     8701 ccgcatcatc agcctgccgg ccgtgaccgt gcagcccatg tggcaaatgc cccagggcct
     8761 cgccaacccg cccccgcccg gggcgccggc ccccaatccc aactaaggcc atgcgaaccg
     8821 ccctgcccgc ctcccggccc cgcgcccgcg ccgccccccg ccccgccccg cccggcatcg
     8881 ccatggtcat cgtcatgatc tcgatcatgg tcttgagcgt gctggcggcg ggatttgcct
     8941 tctccatgaa agtcgagacc aagctggccc gcaacgccaa cagcgaggcc gacctggaat
     9001 ggctgggccg ttccggcgtc gaatacgccc gctgggttct gggcaattcc ctgctcaatc
     9061 cccagcaacc ctatgacagc ctcgatcaac cctgggccac cggctacggc atcctcggcc
     9121 ccaccaacaa ccccatcggc gaagtgcaaa agaccatcga gctcgggcgc ggctccttta
     9181 cctggaccat caccgacctc gaacgcaaat tcaacatcaa ctcgcccgaa cccgtcctgc
     9241 agcaaatcct cccccaggcc ctcaacctca tgggcgtctc cccgggcgaa tccaccgccc
     9301 tgatcaactc catcctggac tggacggatc gcgatgacca aacccacgtc gaaggcgccg
     9361 aatcccgcta ctacgaaagc tcggacccgc cctacttcgc caagaacggc cccattgacg
     9421 acatggccga attgctcttc gtcaaaggga tgacccccga aatctactac ggcctgagct
     9481 ccaccaacta ccagcccagc tactacagcc agcagcgcaa ccgcttcgtc cggggccgct
     9541 ccccggtgcc aaccgtggcg gtcggcctga ccgatctctt taccccgatc tccaccggcc
     9601 gggtcaatgt caacaccgcc cccgtcgaag tcctcatggt cctccccggc gtcaacgaaa
     9661 tcatcgccaa cgccatccac agccgcgcca tggaacccag cgacatcagc ggcctgaccg
     9721 gcccctttcg caccgtggac caactccgat ctctccccga tgtcccgctc gaactggtgc
     9781 ggctgatcca gccattctgc gacgtgcgca gcaaaacctt ccaggtccag atagatgccg
     9841 acgtcggcgg ctataaacgc accttcttcg ccatcctcgg ccgcaacaac cagcgcgacg
     9901 tgcagattct cagcttctac tggcaataac ccctgccgtc agcagccctc cctcccccca
     9961 cacccgcagc ccccataaac aaaagtgtcc aataattgga cactccccaa aacttccccc
    10021 tggttcccct ccatttctcc tatcctcccg cgccatcccc cttgcccaat cccactcctt
    10081 ccccgctcca aaacagccat aaacaagcca tgtacaaggg gtgtacaagg gatgctcacc
    10141 gggagtccag gtagtgtcct aaaagttctg caaaaagcag cgggtcatgg taagccactg
    10201 gg
//
LOCUS       DAOPQG010000126        10049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7619_length_10049_cov_14.535721, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000126 DAOPQG010000000
VERSION     DAOPQG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7619_length_10049_cov_14.535721"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1946)
                     /gene="thiC"
                     /locus_tag="PKI07_12040"
     CDS             complement(90..1946)
                     /gene="thiC"
                     /locus_tag="PKI07_12040"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOC51814.1"
                     /translation="MNAAGTSSEPRSGDPLPHSTRIYVTGEKHPGIRVPFREIKLTPT
                     RAADGTAEPNAPVRVYDCSGPWGDPAFTGSVEHGLPPLRREWIAARGGVEPAPSSYQP
                     VPGRSDAPLPPTLRRQPLRAQPGRAVTQRYYARQGIITPEMEYVALRENLGRALTRPS
                     PAAREGNARGGPPFNAAIPAEITPEFVRSEVARGRAIIPANINHPESEPMIIGRNFLV
                     KINANIGNSALASCIEEEVEKMRWATKWGADTVMDLSTGRNIHETREWIIRNSPVPIG
                     TVPLYQALEKVDGKPEELTWEVYRDTLLEQCEQGVDYFTIHAGVRLRYLPLTARRVTG
                     IVSRGGSIMARWCLAHHQENFLYTRFREICEILRAYDVAFSLGDGLRPGSIADANDEA
                     QFAELRTQGELTRIAWELDCQVMNEGPGHIPLHLIKENMDRQLEWCAEAPFYTLGPLT
                     TDIAPGYDHITSAIGAAMIGWYGCAMLCCVTPKEHLGLPDKKDVKDGVLAYKIAAHAA
                     DLAKGHPAARARDDALSKARFEFRWDDQFNLGLDPVTAREFHDQTLPQEGAKLVHFCS
                     MCGPQFCAMKITEEVRNYAAAQGLAETEALQKGLEHKAQEFIHSGTELYRKA"
     regulatory      complement(1988..2121)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2125..3159)
                     /locus_tag="PKI07_12045"
     CDS             complement(2125..3159)
                     /locus_tag="PKI07_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOC51815.1"
                     /translation="MAELTCTPPPANKPVRISYLFMAGTLVLVGYFRMATPLLVAFFT
                     YLALTHLHFPKRGGKTLAVGLFLVLVAGLVYALAHFAHQSLRALPEIADKAIPSIIEW
                     AQEYQIELPFTDYDSLKEMALDNLGSRMNYLGNFARFAKGATTQFVFVLVGGVVAISV
                     FFNSKLELDREKYAVRDNLYSLSCDQIARRFETLYQSFATVIGAQITISAINTVLTTL
                     FVLAVGLPYAVVVIGVTFLCGLIPVLGNLVSNTVIVGIGFTVSPKMALGALLYLVVIH
                     KLEYFLNSKIIGHRIRNPLWLTLLALVLGERLMGLAGMILAPVVLNYIRMEASCLAVK
                     HSLDSSPDAS"
     gene            complement(3233..6337)
                     /locus_tag="PKI07_12050"
     CDS             complement(3233..6337)
                     /locus_tag="PKI07_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOC51816.1"
                     /translation="MLNWLVQTSLRNRLLVCVLTLALVVVGARLLRVLPIDAFPDTTP
                     VQVQINANVPALSPQEAEQQVTIPVELALGGLPGLQNVRSISKFGLSQVVATFDDRTS
                     IYRARQLISERLQSVDLPAGVPRPQLGPIATGLGEVFHYAVRAKDARRTLTELRELQD
                     WVIKPELRKVPGVAEITSWGGFEKQYHVVADPDLLVKHRLTWEDLFAALEANNQNVGG
                     GQVVRAGESLLVQGIGLTTNVAEIGDIVIASTGGVPVHVRDVATVQVDHEIRRGAVTG
                     GGRGEMVLGLGFMLMGENSHVVTRALKARLAEVRKMLPDDIQLEVLYDRTELVDNVIR
                     TVEHNLLAGALLVIAVLFAFLGNLRAGLVVAAAIPLSMLFAGSLMLQAGISASLLSLG
                     AMDFGLLVDGSVVMVENAMRGLARRRQELGRSLTRRERQEVLARAPLEVVRPVAFGVG
                     IILIVFLPILTLEGVEGKLFRPMALTMVFALAGSLLLALTFIPVAASLFLLRPIKEKE
                     PWLERLARRLYEPVLNLALRFRWFTLLGALGLLAGAGVLAARMGGEFLPRLGEGAIVG
                     TSVRVAGVSVEEAAALNDRLEKLLLAGFPDEIANIWTRLGSADVATDPMGVELSDFFL
                     ALKPRAQWTKARTQAGLVEELQKVFARVPGLRVAISQPIEMRMNELVAGIRSDIGIKI
                     YGDDLEILRGLSDEVQRVLADIPGRGEVTGEQLVGQPVLQVRVDPRATERFGVPARNV
                     LNVVEAVGSRKVGEIREGQRRFPLVVRLPDRQRTDPEALANTHIPTATGSVLPLKQLA
                     QVVETEGPATINREWGRRRITVQCNVRGRDVGSFVAEAQDQIARRVKLPEGYQIEWGG
                     QFENMQRANARLRFVVPLALALILALLYFSLGSMRDVLIVATGIPLGAIGGVAALWVR
                     GMPFTVSAAIGFIALSGVAILNGLVLVTFIRQRLDAGRPLANAVREGCLVRLRPVLMT
                     ALVAAVGFIPMAVNVGIGGEVQRPLATVVIGGALSNTLLTLIVLPVLYCWFHPRQPAA
                     DGSP"
     gene            complement(6350..7612)
                     /locus_tag="PKI07_12055"
     CDS             complement(6350..7612)
                     /locus_tag="PKI07_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC51817.1"
                     /translation="MKTRAYWNTGWLLVLALTLSTSALAHREAPKPEAPGGHAEPGGE
                     AHPAAAKEHCEEHDLLEAECGICHPEIAAQLKPGQGTKVRLPAADSAALAGVELAQAQ
                     RGEIREHLDCYAELTFNQNRLAQIVAPVGGILQSVTADLGTQVKEKQTVATIWSAALA
                     EAVARAVLSHQTLERERKLRAERVTSEKDLQQAEAEHLGACQQLRLQGFTEEQIDRLG
                     REPQEPVLMEVRAPFAGEIVERNAVRGALVEAGKSLFTLTDRSTLWAILSLEEAALGR
                     VRVGQEVELRVDAFPGRTFRGRLTWIAAEVDERSRKARARAEIANPEGLLRARMFAKA
                     RILTRPAEQALLLPPAAFCSLEGKPFVFVKLADDLFEARAVAVGLKDDERWQVAAGLQ
                     PDETVVVNHGFALKSQWLSSRMGAGCAD"
     gene            complement(7609..9012)
                     /locus_tag="PKI07_12060"
     CDS             complement(7609..9012)
                     /locus_tag="PKI07_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC51818.1"
                     /translation="MKRKIYWKWPLLLAVALGGASARPAMSQESGPSADAGALTLEAA
                     VRRALTRNLELSVAESRVASAAGRAAQAKLWPNPELELSAEDWPTEGGGFSEAKKLAG
                     VSQTFPFPGKKKLEGRIGEWGVRLSQAEYDLRRVELVREVKTAFFEVLAAERLTAVAR
                     ELVAVAESSAATARKRVQAGAAADQEQLRAEIPLEQARTELAGFEREEEAARQKLAHL
                     LGSPEWAGAPVSGALAETADLAVLETAPARWLAGHPSVRAARADKERADAAERRARLE
                     PYPDVKFGAAGGQEAGSRASIVQFSLSLPLPLIDRSRGRRQEARADAAAAAAGLAAVE
                     QQLLRDWSTASQRLRTAAQQAAGYRERILPRATEALRLVQRGFEEGKFGFMDLLDTQR
                     TAAEARLSYQQKLLELNIAQANIEALLAAGADEPRGARGTPAPETAWAGPPENPARAG
                     GPAARPITGTDFNPHDL"
     gene            complement(9106..9504)
                     /locus_tag="PKI07_12065"
     CDS             complement(9106..9504)
                     /locus_tag="PKI07_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51819.1"
                     /translation="MRFWRIPAVLVMVTLWVAAGSHCLLAALPGLEFLSCCQHAESER
                     SPAHAEEDCAGDGCSTIESGCYRLEKPQSLPLKPLLASAVVGDAVPGDARPDGAAFLN
                     LRSSSPPELLRAWQFSQRTAQPPRAPSRVS"
BASE COUNT         1662 a         3343 c         3339 g         1705 t
ORIGIN      
        1 ttcgagtccc gttagggtcg ccatctgacc tgttccactt gccggcccaa aggcttggcg
       61 ggaaatgggg cggcgcggcg cgggcgggat caggcttttc gatagagttc ggttccgcta
      121 tggatgaatt cctgcgcttt atgttccagg cctttctgga gggcttcggt ttccgcgagt
      181 ccctgcgcgg cggcgtagtt tctgacttct tccgtgattt tcatggcgca gaactgtggg
      241 ccgcacatgg agcagaaatg gacgagtttg gcgccttcct gcggcagggt ttggtcgtgg
      301 aactcgcggg cggtgaccgg gtcgaggccc aggttgaact ggtcgtccca gcggaactcg
      361 aagcgggctt tgctgagcgc gtcgtcgcgg gcgcgggcgg cgggatggcc tttggcaaga
      421 tcggcggcgt gggcggcgat tttataggcg aggacgccgt ctttgacatc ctttttgtcg
      481 ggaagcccga ggtgttcctt gggggtgacg cagcacagca tggcgcagcc gtaccagccg
      541 atcatcgccg ccccgatggc gctggtgatg tggtcgtagc cgggagcgat atcggtggtc
      601 agggggccga gggtgtagaa cggcgcttcg gcgcaccatt cgagctgccg atccatgttt
      661 tccttgatga ggtgcagggg gatgtggccg gggccttcat tcatgacctg gcaatccagc
      721 tcccaggcga tgcgggtcaa ctcgccctgg gtgcgcagtt cggcgaactg ggcttcatcg
      781 ttggcatctg cgatcgagcc ggggcgcaag ccgtcgccaa ggctgaaggc gacatcgtag
      841 gcgcgcagga tttcgcagat ctcgcggaag cgggtgtaga ggaagttttc ctggtgatgc
      901 gcgaggcacc agcgggccat gattgagccg ccgcgcgaga cgatgcccgt gacgcggcgg
      961 gcggtgagcg ggaggtagcg gagccgcacg ccggcgtgga tggtgaagta atccacaccc
     1021 tgctcgcatt gttcgaggag cgtatcgcga tacacttccc aggtcagctc ttcgggtttg
     1081 ccgtccacct tttccagcgc ctggtacagc ggcacggtgc cgatgggcac gggcgaattg
     1141 cggatgatcc actcgcgggt ttcgtggatg ttccggccgg tggaaaggtc catcaccgtg
     1201 tccgcccccc acttggtggc ccagcgcatt ttctcgactt cctcctcgat gcaggaggcc
     1261 agggcgctgt tgccgatgtt ggcattgatt ttcaccagga agttgcgccc gatgatcatc
     1321 ggctcgctct cggggtggtt gatgttggcg gggatgatgg cgcggccgcg ggcgacttcg
     1381 gagcggacaa actccggcgt gatttcagcg ggaatggccg cgttgaaagg cgggccgccg
     1441 cgggcgtttc cttcccgggc ggcgggcgaa ggacgtgtca acgcgcgtcc caggttttcg
     1501 cggagggcca cgtattccat ctccggcgta atgattccct ggcgggcgta gtagcgctgg
     1561 gtgacggcgc gaccgggttg ggcgcgcagc ggctggcggc gcagagtggg ggggagcggc
     1621 gcgtcgctgc gcccgggaac gggttgatag gacgagggcg ccggctcgac tccaccgcgc
     1681 gccgcgatcc attcccggcg caaggggggc aggccgtgct cgacgctgcc agtgaaggct
     1741 gggtcgcccc acgggccgga gcagtcatag acgcgcacgg gggcgttggg ttcggcggtg
     1801 ccatcggcgg cgcgtgtggg ggtcagtttg atttcgcgga agggcacccg gatgccggga
     1861 tgcttctcgc cggtgacgta gatgcgcgtg gaatggggca gagggtcgcc gctccggggt
     1921 tcgctagagg ttccggctgc attcatagca tggctcaagc acacaagttt ttccggggaa
     1981 cgcgggcggg ggaaacagcg gagttccctt cgccagcatt acctggatca ggttcgacgg
     2041 gtctgccgcg ttcccacgca gcactctcag cctttgcgct gaacccggtt cagcagcggg
     2101 ttggctcccc gataattaat aatactagct tgcgtcggga gaggagtcaa gcgagtgttt
     2161 gaccgccagg caggaggctt ccattcggat gtagttgagt accaccggcg ccaggatcat
     2221 tccggccagc cccatcagcc gttcgcccaa caccagggcc aggagtgtga gccaaagcgg
     2281 gttgcggatg cggtggccga tgattttgct gttgaggaag tattccagtt tatgaatgac
     2341 cacgaggtat agcagcgcgc cgagagccat cttgggggat accgtgaacc cgatcccgac
     2401 aatgacggtg ttgctgacca ggttgcccag aaccgggatc aggccgcaga gaaacgtcac
     2461 cccaatcacc acgacggcgt agggcagtcc caccgcgagc acaaaaaggg tcgtgaggac
     2521 cgtgttgatg gcggagatgg tgatttgcgc gccgatgacc gtggcgaagc tttggtaaag
     2581 cgtctcgaag cggcgcgcga tttggtcgca gctcagcgaa tacaggttgt cgcgcaccgc
     2641 gtatttctcg cggtccaact ccagcttgga attgaagaac acgctgatgg ccaccacgcc
     2701 gccgaccagc acaaagacga actgcgtcgt tgctcctttg gcgaaccggg cgaaattgcc
     2761 caggtaattc atccggctgc ccaggttgtc gagggccatt tccttgaggc tgtcgtagtc
     2821 cgtgaacggg agttctatct ggtattcctg ggcccactcg ataatggacg ggatggcctt
     2881 gtcggcaatc tccggcagcg cccggaggct ctggtgggcg aagtgcgcca aggcataaac
     2941 caggccggcg accaggacga gaaacagccc gacggccagt gttttgccgc cccgcttcgg
     3001 gaaatggagg tgggtcaggg cgagataggt gaaaaaggcc accagcaacg gggtggccat
     3061 gcggaaatag cccaccagca ccagggtccc tgccatgaac aggtacgaga tgcggacggg
     3121 cttgtttgcc ggcggcggcg tacaggtaag ctcggccatt cgccggcatt cataccccgg
     3181 gccctgccgg ggcgcgagct ttttcggtgt tggacggcgg cgggccgggc catcacgggg
     3241 aaccgtccgc cgccggttgc cggggatgga accagcagta gaggacgggc aggacaatca
     3301 gcgtcaggag ggtgttgctg agcgccccgc cgatcaccac ggtcgccagc gggcgctgga
     3361 cttcgccgcc gatgcccacg ttcaccgcca tgggaataaa ccccacggcg gcgaccagcg
     3421 cggtcatcag cacggggcgc agccggacca ggcagccttc gcgcaccgcg ttggcgagcg
     3481 gccggccggc gtccagccgt tggcggatga acgtgacgag gacgagcccg ttgaggatgg
     3541 ccacgccgct cagggcgata aatccgatgg cggcactcac ggtgaagggc atgccgcgca
     3601 cccacagcgc cgccaccccg ccgatggcgc ccagcgggat gccggttgcc acgatgagca
     3661 cgtcgcgcat cgagccgagg ctgaaataca gcagcgccag gatcagcgcc agggccagcg
     3721 gcaccacgaa ccgcagccgg gcgttggcgc gctgcatatt ctcgaactgg ccgccccatt
     3781 cgatttgata gccctcgggc agtttcaccc ggcgggcgat ctggtcttgc gcctcggcga
     3841 caaagctgcc gacgtcgcgc ccgcgcacgt tgcattgcac ggtgatgcgg cggcgccccc
     3901 attcccggtt gatcgtggcc gggccttcgg tttccacgac ctgcgccaac tgcttcaggg
     3961 gcagcaccga gcccgtcgcc gtggggatat gggtgttggc cagcgcctcc gggtccgtgc
     4021 gctggcggtc cggcagccgc accacgaggg ggaaacggcg ctgcccctcg cggatttcgc
     4081 caaccttccg ggagcccacc gcttcgacga cgttgagcac attgcgggcc ggcacgccga
     4141 agcgctccgt ggcccgcgga tccacgcgca cttgcaggac gggttgcccg acgagttgct
     4201 cgccggtgac ttcgccccgg cccgggatat cggccagcac ccgctgcacc tcatcggaaa
     4261 gcccgcgcag gatctccagg tcgtcgccgt atatcttgat gccgatgtcc gagcggatgc
     4321 cggcgaccag ctcgttcatg cgcatttcaa tcggctggct gatcgccacg cgcaggcccg
     4381 gcacgcgcgc aaagaccttc tgcagttcct cgaccagccc ggcctgggtg cgggctttcg
     4441 tccactgcgc gcgcggtttg agcgcgagga agaagtccga caactcgacg cccatcgggt
     4501 cggtggccac gtccgcgctg cccaggcgcg tccagatgtt ggcgatttca tctggaaacc
     4561 cggccagcaa tagcttttcg aggcggtcgt tcagcgccgc ggcttcctcc accgagacgc
     4621 ccgccacgcg gaccgaggtg cccacgatgg cgccttcgcc gaggcggggc aggaattcgc
     4681 cgcccatgcg cgcggccagc accccggcgc cggccagcaa tcccagggcg cccagcagcg
     4741 taaaccagcg aaaccgcagc gccaggttga gcaccggttc atagaggcgg cgcgccagcc
     4801 gctccagcca cggctctttt tccttgatag gccggagcag aaagaggctg gccgccaccg
     4861 ggataaaggt cagggcgagc agcaaggatc ccgcgagcgc aaaaaccatc gtcagggcca
     4921 tgggccggaa cagtttgccc tccaccccct ccagggtgag gatgggcagg aacacaatca
     4981 ggatgatgcc cacgccgaag gcgaccgggc gcacgacttc cagcggggcg cgcgccagca
     5041 cttcctggcg ttcccggcgg gtgagcgagc gccccagctc ctgccgccgc cgcgccaggc
     5101 cgcgcatggc gttttccacc atcaccacgg agccgtccac caggaggcca aagtccatcg
     5161 cgcccaggct cagcaggctg gcggagatgc ccgcctgcag catcaggctg ccggcaaaca
     5221 gcatggaaag cgggatggcg gccgccacca ccagcccggc gcgcaggttg cccagaaagg
     5281 cgaacaacac cgcgatgacc agcagcgcgc ccgccagcag attatgctcg acggtgcgaa
     5341 tcacattatc caccagctcc gtccggtcat acaacacttc gagctggatg tcgtcgggca
     5401 gcattttccg cacttcggcc agccgggctt tgagcgcgcg cgtgaccaca tggctattct
     5461 cgcccatgag catgaagccc agccccagaa ccatctcgcc ccgcccgccg cccgtcaccg
     5521 cgccgcggcg gatttcatga tccacctgga ccgtggccac atcgcgcacg tgcaccggca
     5581 cccctcccgt ggaggcaatg acgatatccc cgatctccgc cacgttggtg gtcaggccaa
     5641 tgccctgcac cagcaaagac tcgcccgcgc gcaccacctg cccgccgccg acattctggt
     5701 tgttggcttc gagcgccgcg aagaggtcct cccacgtcag ccggtgtttg accagcaggt
     5761 cggggtcggc cacgacgtgg tattgcttct cgaacccgcc ccaactggtg atttcggcca
     5821 cgccgggcac tttgcgcagc tcgggcttga tgacccagtc ctgcagctcg cgaagttccg
     5881 taagcgtgcg ccgggcgtct ttggcgcgca ccgcgtagtg gaagacctcg cccagcccgg
     5941 tggcgatcgg ccccaactgc gggcgcggaa cgccggcggg caggtccacg ctctgcagcc
     6001 gttcgctgat gagctgccgc gcccggtaga tgctcgtgcg gtcgtcgaag gtcgccacca
     6061 cctgcgagag gccgaatttg gagatcgagc ggacgttttg cagccccggc aggccgccga
     6121 gggccagctc caccgggatg gtgacctgct gctcggcttc ctgcgggctg agcgccggca
     6181 cgttggcgtt aatctgcacc tgcaccgggg tcgtgtccgg gaaggcgtca atcggcagca
     6241 cgcgcagaag gcgcgcgccc acgaccacca gcgccagcgt cagcacgcac accagcaggc
     6301 ggttgcggag cgacgtttga accagccagt tcagcatgcg cgcctccgct caatccgcgc
     6361 agcccgcgcc catgcgcgag ctgagccact gcgatttcaa cgcgaagccg tgattgacca
     6421 ccacggtttc atccggctgc agccccgccg ccacctgcca gcgttcgtcg tccttgaggc
     6481 ccaccgccac cgcccgcgcc tcgaacaaat catcggcgag cttgacgaag acaaacggtt
     6541 tgccctcgag cgagcaaaag gccgcgggcg gcagcagcag ggcctgctcc gccgggcggg
     6601 tcaggatgcg cgctttggca aacatgcggg cgcgcagcag gccttccgga ttggcgatct
     6661 cggcgcgggc gcgcgccttg cggctgcgct cgtccacctc ggcggcgatc caggtcagcc
     6721 ggccgcggaa ggtccggccg ggaaacgcgt ccacccgcag ctccacttcc tgccccaccc
     6781 gcacccggcc cagggccgct tcctcgaggc ttaaaatcgc ccacaacgtg gagcggtccg
     6841 tcagggtgaa cagcgatttg ccggcttcca cgagcgcgcc gcgcacggcg ttgcgttcca
     6901 cgatctcgcc ggcgaacggg gcgcgcactt ccatcagcac cggttcctgg ggctcgcgtc
     6961 ccagccggtc aatctgttcc tcggtaaacc cctgcaagcg cagttgctgg caggcgccca
     7021 ggtgctcggc ctcggcctgc tgcaagtcct tttccgaggt cacccgctcg gcccgcaatt
     7081 tgcgctcgcg ttcgagcgtc tggtggctca gcacggcgcg ggccacggct tccgcgaggg
     7141 cggcggacca tatcgtcgcc acggtctgct tttctttgac ctgcgtgccc aggtccgcgg
     7201 tcacggattg caggatgccg ccaaccggcg ccacgatttg cgccagccgg ttctgattga
     7261 aggtcagctc ggcatagcaa tcgaggtgtt cgcgaatctc accccgctgc gcctgcgcca
     7321 gttccacccc cgccagcgcg gcggagtccg cggcgggcaa ccgcaccttg gttccctgcc
     7381 cgggcttaag ctgcgcggcg atttccgggt ggcagatgcc gcactccgct tccagcaggt
     7441 cgtgctcctc gcagtgttcc tttgccgccg ccgggtgcgc ctcgccgccg ggctcggcgt
     7501 ggccgccggg agcttcgggt tttggcgctt cgcggtgcgc gagggcgctg gttgaaaggg
     7561 tcaaggccag cacgagcagc cagccggtat tccaataagc tcgggttttc atagatcgtg
     7621 ggggttaaag tcggttccgg tgatggggcg cgccgccggt ccgccggcgc gggcggggtt
     7681 ttcgggcggg ccggcccagg ccgtttcggg cgccggggtt ccgcgcgcgc cgcggggttc
     7741 gtcggccccc gccgccagca gggcttcgat gttggcctgc gcgatgttca gctccagcag
     7801 tttctgctgg taactgagcc gcgcttcggc ggcggtgcgc tgcgtgtcca gcaaatccat
     7861 aaagccgaat ttcccctcct cgaaaccgcg ttgcaccagc cgcagcgcct cggtggccct
     7921 gggtagaatg cgctcgcgat aaccggccgc ctgctgcgcg gcggtccgca gccgctggct
     7981 ggccgtgctc caatcccgca gcaactgctg ttcaaccgcc gccagcccgg ccgccgccgc
     8041 cgccgcgtcc gcccgggcct cctggcgccg gccccgggag cggtcaatca aaggcagcgg
     8101 cagggaaagg ctgaattgca cgatcgaggc gcggctgccg gcttcctgcc ccccggcggc
     8161 gccaaacttc acatcgggat acggttcgag ccgggcgcgg cgctccgccg cgtccgcccg
     8221 ctccttgtcc gcccgcgccg cccggacgct ggggtgtccc gccagccagc gcgccggcgc
     8281 ggtctccagc acggccaggt ccgcggtctc cgccagggcg ccgctgaccg gcgcgcccgc
     8341 ccattcaggg ctgcccagca agtgggccag cttctggcgc gcggcttcct cctcccgctc
     8401 gaagcccgcc agttcggttc gggcctgttc gaggggaatc tccgcgcgca gttgctcctg
     8461 atccgccgcc gcccccgcct gcacccgctt gcgggcggtc gccgccgacg actcggccac
     8521 ggccaccagc tcgcgcgcca ccgccgtcag gcgctccgcc gccagcacct cgaagaaggc
     8581 ggttttcact tcccgcacca gttccacgcg ccgcagatca tactccgcct ggctgagccg
     8641 gacgccccat tcgccgatcc gcccctcgag ctttttcttg cccgggaacg gaaacgtctg
     8701 cgacaccccc gccagctttt tcgcctcgga gaaaccgccg ccctcggtcg gccagtcctc
     8761 ggcgctcagc tccagttccg ggttgggcca cagcttcgcc tgggcggcgc gcccggcggc
     8821 gctcgccacc cggctttccg ccacgctgag ctcaagattg cgcgtcagcg cccgccggac
     8881 tgccgcctcc agcgtcagtg ccccggcgtc cgccgacggc ccggattcct ggctcattgc
     8941 cgggcgcgcg cttgcgccgc ccagcgccac cgccaggagc agtggccatt tccaataaat
     9001 ctttcgtttc ataaacaaac taaacaggct taaaccaata ccaacggaat cggccggagg
     9061 cgtggaaccc cgccatccgg acggagaagt cccgaccggg actgtttaag aaacgcggga
     9121 aggcgcgcgc ggcggctggg ccgtgcgctg agagaattgc caggcgcgga gaagctcagg
     9181 cggagaggaa gaacgcaagt tcaggaaggc cgccccgtcc ggccgggcat ccccgggcac
     9241 ggcgtccccg accaccgccg acgccagcaa gggcttgagc ggcagggatt ggggcttttc
     9301 cagccggtag cagccggact cgatcgtcga gcagccatcg ccggcgcaat cctcttccgc
     9361 gtgcgcgggc gagcgttcgg attccgcgtg ctggcagcag gagagaaact ccagtccggg
     9421 aagcgccgcc agcagacaat gcgaccctgc cgccacccaa agggtgacca tcaccagaac
     9481 cgcgggtatc cgccagaatc gcatcagacg ttttatagat gccctcattg tggcggaagt
     9541 tcaagttttt atcgccctca ggctaattga tcaccccgcc tgccgtcagc cttgaccgcc
     9601 gggtgtggag gatgaggagg cattgcggct tgacgcccgc aaccgaacgc cgggccaaag
     9661 gctattcggg ccggtcggga atggatttaa ccaaaacgac tcagatttca acctcaatcc
     9721 gacttcacag aacgcgcaag tcactgattt tacgggcaaa aagcatttgt aagccagtgg
     9781 ccaacccgtt gaatacgcca accaaacagt tcattgaggc tctccgctgg cttgtcaggc
     9841 ccgtttcggc tccaatagag cgagggcgca cggttggaat gtgtagcgtc aacgtttcag
     9901 ataaaggatt cgcgaagcca aaacccattc ggacactcgc ccgcccctga aaagccgcct
     9961 tttgtcagcc ctggattcat aactaactgt ttttacagtt gtgtccgtca agttcagcaa
    10021 attggtcgtc atggtttcca ctcaaggtg
//
LOCUS       DAOPQG010000127         9700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_7944_length_9700_cov_15.585070, whole genome shotgun sequence.
ACCESSION   DAOPQG010000127 DAOPQG010000000
VERSION     DAOPQG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7944_length_9700_cov_15.585070"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1661
                     /locus_tag="PKI07_12070"
     CDS             351..1661
                     /locus_tag="PKI07_12070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOC51820.1"
                     /translation="MRILLAGDLHLGRSSSSVRGDCAGHRAADVWQRLVDLAIEQGIG
                     AVILSGDLVDQSNRYFEAIGPLANGLRRLAAQEISTIAVAGNHDYHVLPGLAQQYADE
                     KHRFTLLGQGGNWECHPVVRGGQTVFRVVGWSFPAESHDRDPLDAFPANLPDDLPVLG
                     LVHGDLDARGSRYAPLSGAALRAKPVAGWLLGHIHAPHCDSQAGAPWLLYPGSPQALD
                     PGEQGAHGVWVAEVTPTAITLPVQVALSSVRYEGVTVNADSVADAQELRVRLRDRLRA
                     EAETARQAGGESLRQLVVDLVIEGAAALAAREVDQVMEELREARELDTAVAVEVRKTD
                     NRVVPPLDLEGLARGKALPGILARLVLDLEAGRNSEAVERVRARARRAVNDLTFMSQV
                     REGDSESTFQLSDDALRQSLIQAARKLLGSLEPTGAADAGGGLP"
     gene            1658..5170
                     /locus_tag="PKI07_12075"
     CDS             1658..5170
                     /locus_tag="PKI07_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC51821.1"
                     /translation="MNGKFARFTRVEIVKALGIGRGQGFALEELSPRINIIHGPNGSG
                     KSTLLRVMQKLLWPQASEVEPALARATVSGALEITDAQQTREGRVEIEAGHVTSAIDG
                     ASGLLPVGRPDLHARYRMPVAELLVDDDRAFAREIHRQAAGGFDLEECAARAGLAAGP
                     TRPQALQRERREAVERVRSARDRQVELDRSRTELETQQEKRRQLEEQESARDDLERAL
                     RHLEAEQEEEQKREEREAFDAVVRQLYGGEDRERARLEQAEQDARRALDSHQEKLAEQ
                     EDLIAGVGITEAGRAADFPALEAALATLRAAEQRAQEDARAMNQDATALAQAAAALFD
                     RAEAVQAGIIDQAVLGEAQALFEQALALRALQRECETERERLELLPAAAESLPEAGVL
                     ARGIEALANWLRAPALPADLPAPFPLVKLGVVCVLLAAASLVGAGLVHWGFAAGALVA
                     AAVFGVAAWLSGRDRSRDRQIAARADAAATHRETYAGLSLPDPAPWSVESVRAVLGEL
                     IEWQRRRVDQDAMGQARLVHQRRAAQLQQGQEQLAGRCVDFEQRHGIGAGDPDKTWIC
                     LRVQRINDWQQATQRHAASRAAAADSDRALALAREACRARLEAAGVTGAGDLPLTFDF
                     ASARIDHLRKQRCRWQELHQARQRLEKETAGLTEARDQAAANLAAFWRGLGLAEGDLA
                     GLARLLEARERFAAATRDWELAVARVKQTRRELGKAEELLARGLAQIKSDLKAAAAGR
                     EELASLRKEIAKKEAELEQAARGYDVSERLEELAAANAKLLAQEERNAELAVGGALVA
                     WLRDQIRAELEPPVLARARRLLSQFTRGRLTFEVEDPGGGGVQLMAASGGEARRPITD
                     LSSGERVQLLMAVRLAFLEESEERSLPLLLDEVLAACDDERTRNIIAAVIEIARSGRQ
                     VFYFTAQNDEVGKWRACLAEAGMAGRVIDLQAVRAGAEASGRPLPPPPPLRPAWPAPD
                     DCSHRQYGERLQVPGVDPWDPNLDQLHIWHVIEDVPLLYRVLSSDIERLGPLERMVQN
                     AGYTELGEARERVFATGAAFRYACRAWRIGRGKPLTREALVDADGVSRVFLDRVCDLS
                     QRVGYDARRLLRELESGGLRNWREQATQELRNYLMREGYLDEAQPLTPDQIREKVREE
                     LCQDGLGDLVTPATLDRVIASLPA"
     gene            complement(5240..5758)
                     /locus_tag="PKI07_12080"
     CDS             complement(5240..5758)
                     /locus_tag="PKI07_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51822.1"
                     /translation="MQTRWPARCAWILSTAAACLCLCFGSALAYADPAAAPAQPGNPA
                     AAPANPAAAETEIPQSVFINPTTPQEGRDPFFPNSRRRIKTHVIVPTNAPVTVVELEL
                     KGISGSADRPLAIINNRTFEPGEEGTVVSKQGRIRVICKEILPDSVRVIFNGQERTLT
                     LRSLSPMDKSRP"
     gene            complement(5775..6365)
                     /locus_tag="PKI07_12085"
     CDS             complement(5775..6365)
                     /locus_tag="PKI07_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51823.1"
                     /translation="MNFRKLPKEKRNQLVLVVLLTLMAVIGLYYTLIRHQNESLAKLA
                     QQKTAAAQKLQVVQDTLRRAQPIQAELDEIRKTLTVAERDVASGDLYAWVINTINKFK
                     ASYPAVELPQFSQLGQAVEVNLFPSFPYKQAVLTIGGTAHFHELGQFIADFENQHPHI
                     RLVNLSLDATSASPLAESEMLAFKMDIVTLVKSNPS"
     gene            complement(6362..7036)
                     /locus_tag="PKI07_12090"
     CDS             complement(6362..7036)
                     /locus_tag="PKI07_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51824.1"
                     /translation="MIRLNLLAEAQAAEELRRRDPVKRAIWLAVLIVALMLAWSSSLQ
                     LKSILGHSEISRLEADIKSHTSQYELVLANQKQIADIKQKITGLQQLTNNRLLNGTLL
                     NALQQTTFDEVQLLRLRVEQLYTTVAGTKARTNESGTLIPGKPATATETIRLTLEGGD
                     SSDNPGDGLNKYREALANAPYFKQNLVPTNGITLKSLTPPEISALTGKRSVIFSLECH
                     YPEVTR"
     gene            complement(7051..8076)
                     /gene="pilM"
                     /locus_tag="PKI07_12095"
     CDS             complement(7051..8076)
                     /gene="pilM"
                     /locus_tag="PKI07_12095"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOC51825.1"
                     /translation="MAVDLGSRTTKAVHVARRGQGFALCGYTLLDAPIYEKNLPPDLL
                     SEHLKTVHQILRSRTRHVCLTVGANEAQVRHAELPNMPVKDMRQVLKLNSKSYLQQDL
                     SGHVFDCHPCAPARANQTNNKASAPAGLAKQKVLVAAARQQFVDDFSQGIKGAGLIAA
                     CITPALLGPVNAFEKVMPELFEVGTVALVDLGFKNSSICLLRDGELLLSRVVAIGGDR
                     MTADLAENLGISYAEAEGIKIGMPQEVQTQLEALLLPLGRELRASIDFFEHQQDQAVS
                     LAFVTGGPAQSEFIMETLQREIMIDCKLIKPTSFLQLELPPQQAVEIEQVAPQLTVAL
                     GAALAAL"
     gene            8610..9023
                     /locus_tag="PKI07_12100"
     CDS             8610..9023
                     /locus_tag="PKI07_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC51826.1"
                     /translation="MKAKKILVVEDDKVAQKLIVDALKAAGYEVATAYDGATAVAKAR
                     ELEPDLVTLDIQLAKDSPDDTWDGFSVATWLRRISESKSKPVIIVISGLEPDQIIESA
                     ANIGAYTFLPKPFTKQKLLDLVAEALKATPEASAD"
     gene            9220..>9700
                     /locus_tag="PKI07_12105"
     CDS             9220..>9700
                     /locus_tag="PKI07_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC51827.1"
                     /translation="MRFGINTFLFTSPFTNDSTRWFRQFKQWGFDSVEIPIEDPAHID
                     PAYVKRELDRHGLVCGSVCACLGPDRDLRGTAQQQRNSLDYLKRVIDQMVVLGSRSVV
                     GPVYSSVGRAEAVPPAEYRKQWKTVVKHLKVLAAYAQQRGKQICLEPLNRFETDFINT
                     "
BASE COUNT         1672 a         2645 c         3595 g         1788 t
ORIGIN      
        1 atcgagtaat ttcataagcg tgaaacttcc atagttgttt gattgccaag ccgggtaggc
       61 ggcccggcgg cccgcgcacg cgggtcagtt aaccgggcag cggtttgtcc ccagccaccg
      121 gaaaaagggt atcaggccgg aggcggcggt caacgaagat agcgattgca gaaagggctc
      181 ccagcgttga caggggtgga ggcgatcgca gtgagccggg ttgcgcggaa gggccggaaa
      241 aagaggcggc gatgcgaggg gcaaaaggaa ggcgggaact tcggctgatt ccgcgtggag
      301 ccgcaaattg aattgtcgtt ccgggggaaa atatttatag gataatttct atgcgcatcc
      361 tgttagccgg agatttgcat ttgggcaggt caagctcgag cgtgaggggc gattgcgcgg
      421 ggcatcgcgc cgcggatgtt tggcagcgcc tggttgatct ggccattgag cagggaatag
      481 gcgcggttat cctgagcggc gatttggtgg atcaatccaa tcgctacttc gaggctatcg
      541 gcccgctcgc gaatgggctt cggcggttgg cggcccagga gatcagcacc attgccgtgg
      601 caggcaatca tgattaccac gtgctgccgg gactggccca gcagtacgcg gacgagaagc
      661 atcgcttcac tcttctggga caggggggga attgggagtg tcatccggtt gtcaggggtg
      721 gccagacggt ctttcgcgtg gtggggtggt cgtttccggc ggaatcccat gaccgcgatc
      781 cgctcgatgc ttttcccgcg aacctgccgg acgatctgcc ggtgctcggg ctggttcacg
      841 gcgacctgga cgcccggggt tcgcgctatg cgcccttgag cggggccgcg ctacgggcaa
      901 agccggtggc gggttggttg ctcggccata ttcacgcgcc ccactgcgat tcgcaggccg
      961 gcgcgccctg gctgctatat cccggcagtc cccaggcgct tgaccctggc gagcaggggg
     1021 cgcatggcgt gtgggtggcg gaggtcacgc ccacggccat aactctcccc gtccaggtgg
     1081 cgttgtcttc cgtgcgctac gaaggcgtca cggtgaatgc cgattccgtg gcggacgccc
     1141 aggagcttcg ggttcgtttg cgcgatcggc ttcgggctga ggccgaaacg gcccgccagg
     1201 cgggcggaga gagcttgcgg caactggtgg ttgacctggt gatcgagggg gcggcagcgc
     1261 tggccgcccg cgaggttgat caggtcatgg aagagctgcg ggaggcgcgc gagttggaca
     1321 ccgccgtagc cgtcgaggtg cgcaagacgg acaaccgggt ggtgccgccg cttgatctcg
     1381 aaggactggc ccggggcaaa gcccttccgg gcatactggc ccggttggtg cttgacctgg
     1441 aagccgggcg gaattcggag gcggtggagc gggtcagagc cagggcgcgc cgggcggtta
     1501 atgatctgac gttcatgtcg caggtcaggg agggggacag cgaatccacc ttccagttga
     1561 gtgatgacgc cctgcggcaa agcctgatcc aggccgcccg gaagctgctg ggcagcctgg
     1621 agcccaccgg cgcggccgat gccgggggag gtttgccatg aacggcaagt ttgcgcggtt
     1681 tacccgggtg gaaatcgtca aggcgctggg gatcggccgg ggccagggtt ttgccctgga
     1741 ggagctttcg ccgcggatca acatcatcca cggccccaat ggttccggga agtcaacgct
     1801 tttgcgggtc atgcagaagt tgctgtggcc ccaggcttcg gaggtggaac cggcgctggc
     1861 gcgggccacg gtgtccggcg cgctggaaat cacggacgcc cagcagacgc gcgaggggcg
     1921 ggtggagatc gaggcggggc acgtcacgag cgcgattgac ggcgcgagcg ggctgctccc
     1981 ggtggggcgc ccggacttgc atgcgcgcta ccggatgcct gtcgcggagc tgctggtgga
     2041 cgatgatcgg gcttttgccc gggagattca ccggcaggcg gcgggagggt ttgacctgga
     2101 ggaatgcgcc gcccgcgccg ggctcgccgc cggccccaca aggccacagg cgctgcagag
     2161 ggagcggcgc gaggccgtgg agcgggtccg gagcgcgcgg gacaggcagg tggagttgga
     2221 ccggagccgc accgagttgg aaacgcagca ggaaaagcgc cgccaactgg aggaacagga
     2281 gtctgcccgg gacgacctcg aacgcgcgct aaggcacttg gaagccgagc aggaggaaga
     2341 gcagaagcgg gaggagcgtg aagcttttga cgccgtggtg cggcaactct atgggggaga
     2401 ggatagggaa cgggcacggc tggaacaagc agagcaagat gcccgccgtg ccctggacag
     2461 ccaccaggag aagctggccg agcaggaaga cctgatcgcc ggcgtcggga tcaccgaggc
     2521 cgggcgggcg gcggattttc cggcgctgga agccgcgctc gcgactttgc gcgccgccga
     2581 gcagcgggcg caggaggatg cgcgggccat gaaccaggac gccaccgcgc tggcgcaggc
     2641 cgccgcggca ctgttcgacc gcgccgaagc cgtccaggcc gggattatag atcaagccgt
     2701 gcttggggaa gcccaggcgc ttttcgagca ggcgctggcg ctgcgggccc tgcagcggga
     2761 gtgcgaaacg gagcgggaac gcctggagtt gctgcccgcc gccgccgaga gcctgcctga
     2821 ggcgggagtg ctggcccgcg gcatcgaggc gctggcgaac tggctgcgcg caccggcact
     2881 gccagccgac cttcccgcgc cgttcccgct ggtcaagctg ggagtggttt gcgttcttct
     2941 ggcggcggct tccctggttg gggcggggct ggttcactgg ggattcgcgg ccggggcgct
     3001 ggtggcggcg gccgtttttg gcgtggcggc ctggctatcc gggcgcgacc ggagccggga
     3061 ccggcagatc gcggctcggg cggatgcggc cgcgacgcac cgcgagactt atgcgggctt
     3121 gtccctgccc gatccggccc cctggtcggt tgaatcggtc cgcgcggtgc tcggcgaact
     3181 gatcgagtgg cagcggcggc gagtggatca ggatgcgatg gggcaggctc gcctggtcca
     3241 tcaacgccgc gcggcccagc ttcagcaggg gcaggagcag ttggccggcc gctgcgtgga
     3301 ctttgaacaa aggcacggca ttggggcggg cgaccccgac aaaacatgga tttgcctgcg
     3361 ggtccagcga atcaacgatt ggcagcaggc aacgcagcgg cacgccgcga gccgcgcggc
     3421 ggcggcggat tccgaccggg cgctggcgct ggcgcgggag gcgtgccggg cgcgcctgga
     3481 ggcggccggc gtgacaggag cgggcgattt gccgctgacc ttcgatttcg catcagcgcg
     3541 cattgaccac ttgcggaagc agcgttgccg ctggcaggag ttgcatcaag cgcgccaacg
     3601 gctggaaaag gaaaccgcgg ggttgactga ggctcgcgat caggccgcgg caaacctggc
     3661 cgctttttgg cgggggcttg gcctggccga gggggatctg gcaggcctgg cccggttgct
     3721 ggaggcgcgg gagcgatttg cggcggcaac gcgtgactgg gagctggcgg tcgcgcgcgt
     3781 caagcaaacg cggcgggaac tgggcaaggc agaggagttg cttgcgcgcg ggcttgcgca
     3841 gatcaaatcc gatctgaagg cggctgccgc ggggagagag gagttggcct cgttgcgcaa
     3901 ggagattgcc aaaaaggaag cagaactgga gcaggcggcg cggggctatg acgtttcgga
     3961 acggctcgag gaactggcgg cggccaatgc gaagctgctt gcccaggagg aacggaatgc
     4021 cgagctggcg gttggcggcg cgctggtggc gtggctgcgg gatcagatcc gcgccgaact
     4081 ggaacctcca gtcctcgcgc gggcgcgacg attgctgagc caattcaccc ggggccggct
     4141 gactttcgaa gtcgaggacc cgggcggcgg cggggtgcaa ttaatggcgg ccagtggcgg
     4201 cgaagcgcgc cggcccatta cggatttgtc ctcgggcgag cgggtgcaac tgctgatggc
     4261 ggtgcgcctg gcgtttttgg aggagagcga ggagcggtcg ctgcccctgc tgctggacga
     4321 ggtgctggct gcctgcgatg acgaacgcac ccgcaatatc attgccgccg tgattgaaat
     4381 tgcgcgttcg gggcggcagg tgttctactt taccgcccaa aacgacgagg tggggaagtg
     4441 gcgggcttgc ctggccgaag cgggcatggc gggcagggtg attgatttgc aagcggtgag
     4501 ggccggggcc gaagcgagcg gccgcccgct gccgcccccg cctcctttgc ggcccgcctg
     4561 gccggcgccg gacgattgct cgcatcgtca atatggagag aggttgcaag tgcccggggt
     4621 ggacccctgg gatcccaatc tcgaccagct tcatatttgg cacgtgatcg aggacgtgcc
     4681 gcttctttac cgggttctta gctcggatat tgaacggctg gggccgctgg agcgcatggt
     4741 ccaaaacgcc ggctatacgg aattgggaga ggcccgcgag cgggtgtttg cgaccggcgc
     4801 cgccttccgt tacgcctgcc gggcctggcg catcgggcgg ggcaagccat tgacgcggga
     4861 agcgctcgtt gatgcggacg gcgtgagcag ggtgtttttg gatcgggttt gcgatctttc
     4921 ccagagagtg ggttacgatg cccggaggct tctccgggag ttggaaagcg gaggactcag
     4981 gaactggcgc gagcaggcaa cgcaggaact gcgcaattac ttgatgcgcg agggctatct
     5041 tgacgaggct cagccgttga cgcccgacca gatacgggaa aaagtccggg aagagttgtg
     5101 ccaggacggg ttgggcgatc tcgtgacgcc ggcgacgctt gaccgcgtaa ttgcctcatt
     5161 gccagcgtga gccgggcacg gaaaggccgg ggcggtttgc aactgatgcg gaccgcggag
     5221 gagggaaccg gggacggagt cagggacggc tcttgtccat cggggacagg ctgcgcaaag
     5281 tcagggttcg ttcctggccg ttgaaaatga cccggacgga atccgggagg atttccttgc
     5341 agatgacgcg gatgcggccc tgcttgctga ctacggtgcc ttcctcgccg ggctcgaagg
     5401 ttcggttgtt gatgatggcc aaggggcggt cagccgaacc ggaaatgccc ttcaattcca
     5461 gctccacgac ggtgaccggg gcgttggtgg gcacgatcac gtgagttttg atgcgccgcc
     5521 gggaattggg gaaaaagggg tcgcgtcctt cctgtggggt cgttgggttg atgaagacgg
     5581 actggggaat ctccgtttcg gcggctgcgg ggttggccgg cgcggcggcg gggttgccag
     5641 gctgggccgg cgcggctgcg gggtcggcgt aggcgagggc gctgccgaag cagaggcaaa
     5701 ggcaggcggc ggcagttgag agaatccagg cgcaacgcgc gggccatcgg gtttgcataa
     5761 ggcgcatgag aaggtcagga ggggttgctt ttgaccaggg tgacaatgtc catcttgaag
     5821 gccagcattt ccgattccgc cagcggggag gcggaagtgg cgtcgaggct gaggttcacg
     5881 agccggatat gggggtgttg gttttcgaaa tcggcgatga actggcccag ttcgtggaag
     5941 tgagcggtgc cgccgatcgt cagcaccgct tgtttatagg ggaaactggg gaacaggttg
     6001 acttccaccg cctggccgag ctggctgaat tgcgggagtt caaccgcggg ataggaggct
     6061 ttgaacttat tgatcgtgtt gatcacccag gcgtacaggt cgcccgaggc tacatcgcgc
     6121 tccgcgacgg tgagggtttt gcggatttca tccaactcgg cctggatggg ctgggcgcgg
     6181 cggagggtgt cctggacgac ctggagtttt tgggcggccg ccgttttttg ctgcgcgagc
     6241 ttggcgaggc tttcgttctg gtgccggatc agcgtgtagt agaggccgat gacggccatc
     6301 agggtgagca ggacgaccag gaccaactgg ttgcgttttt ccttggggag tttgcggaag
     6361 ttcatcgcgt tacctcgggg taatggcatt ccagcgagaa gatcacgctc cgtttgccgg
     6421 tgagcgcgga aatctcaggg ggcgtcaggc ttttgagcgt gatgccgttg gtggggacca
     6481 gattttgttt gaagtaaggg gcgttggcca gcgcttcccg gtacttgttc agcccgtcgc
     6541 cggggttgtc gctggaatcg ccgccttcga gcgtgagccg gatggtttcg gtggcggtgg
     6601 ccggcttgcc cgggataagg gtgccgcttt cgttggtgcg ggccttggtg ccggcgacgg
     6661 tcgtgtaaag ctgttcgacg cgcaggcgga gcaactggac ttcatcaaaa gtggtctgct
     6721 ggagggcgtt gagcagcgtg ccgttgagga ggcgattgtt ggtcaactgc tgcagtccgg
     6781 tgattttctg cttgatgtcc gcgatctgct tttggttggc gaggacgagc tcgtattggc
     6841 tggtgtggga tttgatgtcg gcttcgaggc ggctgatttc gctgtggcca aggatggact
     6901 taagctgcag ggagctgctc caggccagca tgagcgccac gatcaggacc gccagccaga
     6961 tggcgcgctt gacggggtcg cggcgtcgca gttcttcggc ggcctgggct tcggcaagaa
     7021 gattaaggcg aatcataaat ccagtcgggg ttagagagcc gccagggcgg cgcccagcgc
     7081 gacggtaagc tggggcgcga cctgctcgat ttcaacggct tgttgcggcg gcagctccag
     7141 ttgcagaaaa gaggttggtt tgatcagctt gcagtcgatc atgatttcgc gttgcaacgt
     7201 ctccatgatg aattcggact gggcgggccc gccggtgaca aaggccaggc tgacggcctg
     7261 gtcctgctgg tgttcaaaga aatcaatgga ggcgcgcagt tcgcggccca ggggcagcag
     7321 gagggcttcg agttgggttt ggacttcctg gggcatgccg atcttgatgc cctcggcctc
     7381 ggcgtagctg atcccgaggt tttcggccag gtcggcggtc atgcgatccc cgccgatggc
     7441 cacgacgcgg ctgagcagca gctccccgtc ccgcaggagg cagatggagg aattcttgaa
     7501 gcccaggtcc accagggcca ccgtcccgac ctcaaacagt tcgggcatga ctttttcaaa
     7561 cgcattgacg gggcccagca gggccggggt gatgcatgcc gcgatcaggc cggcgccctt
     7621 gatgccctgg gagaagtcgt ccacgaactg ctggcgggcc gcggcgacga gcactttttg
     7681 cttggcaagg ccggcggggg cggaggcttt gttattcgtc tggttggccc gcgccggggc
     7741 gcagggatgg caatcgaaca catggccgga caagtcctgc tggaggtagg atttggaatt
     7801 gagtttgagg acctggcgca tgtccttgac gggcatgttg ggaagttcgg catggcgcac
     7861 ctgggcctcg ttggcgccga ccgtgaggca gacatgccgg gttcgggaac ggaggatttg
     7921 gtgaacggtc ttgagatgct cgctgagcaa atccggcggc agatttttct catagatggg
     7981 agcatccaga agggtatagc cgcagagggc aaagccctgg ccgcgacgcg ccacatgcac
     8041 cgccttggtg gtgcggctgc cgagatccac ggccattatt tggtcgcgtt tctggccgat
     8101 tgctctgtca aaaaaaggca gacccatgcg cgttacctat gcatttactg tttttccaca
     8161 tgccgccgat gcttatcacg tttccacaat gctggcgcgc ggtgcggcct gttccgcgtg
     8221 cagccgtctg gttaaggggc attcctgccc caaaacgtga accaattccg agttgtcctt
     8281 ggtagcacat ccagggccaa aattggcgtt gcggatttcg accgttggcc cgcgggtggc
     8341 ggccccgccc atttgcggag ggcgtgcttg cgcggcgagg agaggaactg gcggttttcg
     8401 gacaatccgt tgccctgttt cccgcgcggc gccgtgcggg gcgtcttatc attcgacgag
     8461 tgtgtctccg gatgagacat tcctgtcccg ggctgccacc cgcttgcccg gtttgccagt
     8521 ttgcggcctg cgcgccgaag gatgcgagct tgacggcggg aatcccccgg ggccatatta
     8581 ggcgcaaccg cagcgaatgc cacgcaccga tgaaggccaa aaaaatactg gtagtagaag
     8641 atgataaagt agcgcagaag ctgattgtgg acgcgttaaa ggcggccggc tacgaagtgg
     8701 ccacggccta tgatggcgcc accgcagttg ccaaagcccg agaactggag ccggacctgg
     8761 tcactctgga cattcaacta gccaaggatt cgcctgacga tacctgggat gggtttagtg
     8821 tggctacctg gctgcgtcgc atcagtgaaa gcaagtcaaa gcccgtgatt atcgtgattt
     8881 ccgggctgga acccgaccag atcattgaga gtgccgccaa tattggcgcc tatactttcc
     8941 tgcccaagcc atttaccaag cagaaattgc tggacctggt cgccgaggcc ttgaaggcaa
     9001 ctcccgaagc ttccgcggat tagcgccagg cctttcgttc ggccggacgg aatcgcgggc
     9061 gacgaaagga atccccgctg gatcggagga ggctcgccgt gggattgcgc ggggtttgtg
     9121 cctgcgagat tcggcctgac gagttgcggc gcggccggag gcacggttgg agataaggct
     9181 ttgactcggc ggcgcgatgc ggtagccttg ccggtgatta tgcgatttgg cattaataca
     9241 tttctcttca cttcgccgtt tacgaacgac agcacccggt ggttccggca attcaaacaa
     9301 tggggattcg actcggtcga gatacccatc gaggacccgg cgcatattga tccggcgtat
     9361 gtgaagcgcg aactggaccg gcacgggctg gtctgcggct cggtttgcgc ttgcctgggg
     9421 ccggatcgcg atttgcgtgg cacggcccag cagcagcgga acagccttga ctacctgaag
     9481 cgggtgatag accagatggt cgtgctcggt agccgatcag tcgtcggccc ggtctattca
     9541 tccgtgggcc gcgcggaggc ggtgccgccc gctgaatacc ggaagcaatg gaaaacggtg
     9601 gtgaagcacc tcaaggttct ggccgcgtat gcccagcagc ggggaaagca gatttgcctg
     9661 gagccgctca accgcttcga gacggatttc atcaacacct
//
LOCUS       DAOPQG010000128         9411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_8254_length_9411_cov_15.354104, whole genome shotgun sequence.
ACCESSION   DAOPQG010000128 DAOPQG010000000
VERSION     DAOPQG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9411
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8254_length_9411_cov_15.354104"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            617..1684
                     /locus_tag="PKI07_12110"
     CDS             617..1684
                     /locus_tag="PKI07_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOC51828.1"
                     /translation="MRNLWRWIAFAWLSGLATLPASAAFSSIYVLGDGVCTTTASPSP
                     PSLFYGNRFCNGRVWVEVLAQRQGLTLPTSQNQSYFGHTSTNLLALVSGHSFTLPPAA
                     TNWLFVVWVCDADFVGDIENVPFNSSTWDKYVNASLTNHFRAVTNLYAKGARTVIMPN
                     AVDITMVPNYYERPNKAFIRQKVINFNTGLASAVASQIKAKCPLITLYIPDFFALFDS
                     MLATPATYGLSNPSPGCYAIGDGHTSLSSGPGVSYIFWDDTDPTAKAQAIMADVAQQM
                     ISPATFSRVTTQGASLRLDVVNLPVGLSGSVESRSQAGAGAWSPVASFNSTNLTQALY
                     VTPAGARQFYRLRFPFAWTWP"
     gene            complement(1777..4812)
                     /locus_tag="PKI07_12115"
     CDS             complement(1777..4812)
                     /locus_tag="PKI07_12115"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOC51829.1"
                     /translation="MRFARALGRFLVRGSLGLALAGAGSLPAAMAPYPPDADTLHLWH
                     LDEPASASSATNAVAGGVALTAIANGASLAHPALPGFGSAASTYDGGPGATLAANPNG
                     VPGRDAYLGPLPFVNGPGDNALLVCAGTNGAFTFEALVRADFGPTNLAVAPDKSRTMQ
                     ILSADAEETLRLFQFRIVWARTNDPNPQLQFINIGAAPPGIQTLMAYLPMSGSNALGA
                     SNWYHVAVSYDGRPNTAGNLKLYWTRAEPGRTRADLLASLRMTNNLPTGSADWGLGNE
                     GRATGGSDGNWVGLLDEARISSVARGPDEFIFFADTDGDGLPDAWELKHFGSLARGPA
                     DDPDGDGFSNWQEYRGGSNPARKASTPGDKDADGLPDVWEITCFGSLALGPADDPDGD
                     GFTNAEEWAAGTNPADAASSPADTDGDGLPDAWEIAHFGSLDLGPEDDPDDDGFSNRQ
                     EYRAGTNPADPHSCPPGPRVRFIPIEDGDPDTSEYAYAGGGINTASFIRSSLMTVGEQ
                     QFITYYGRHQTNPNYSFNNTLWVARRAITTNRWEVFRTSFTANNIADGHDCISMGFDG
                     EGYMHLSWGMHGDALHYARSTAPVLGNGPIAFGPDGTMTGQETAVTYPQFITLPGGDL
                     LFLFRKGSSGNGDTFLNRYRLIHHSWVNLHTDGVQSRPFIKGTGWTPNYNAYWQMPCL
                     DAAGNLLLVWTWRYNADSPKKEVGYQTNHDFAYARSANGGITWLRSDGTPYALPISES
                     GENGDPNTIAEKVLDIPEGSSLMNQSGMCLDQSGQPVIANWWAPGAATNNHRRQYLVA
                     FPAAAGWETRQLSHRTLDSPAYKVPETALGDMGRPTILCDRDDRLIVIYRDNEGSNGL
                     TVVHSLPRAQDPARQVWTAFDLTTENLGRFDAPNVDLPRWERDNVLHIFYQPVNGLGY
                     AAPANTAAPIGVLEWDAAAYFAHRPFLQVSRSGTADATLAFNAQPGWTYQVQTSTNLS
                     DWDTLATLAGIAGRLQYVHTNGVSASPRFWRLRLHEGSD"
     gene            complement(4872..5687)
                     /locus_tag="PKI07_12120"
     CDS             complement(4872..5687)
                     /locus_tag="PKI07_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51830.1"
                     /translation="MRKLVGPVIRRPRPVPGRAPAALAAGNFPRHGFTLIELLVVIAI
                     IAILAALLLPALASAKEKATRTQCLSNLRQIAVAMNVYALDNADWLLPAWEQQGKFVQ
                     LVISPENSEVAKSVGLINSTNTANVWACPARPGLPQVNRYNQYALGYQYLGGVKTWLN
                     FATGGAEVKSRSPIKMGLSQPRWVLAAEANVKYIPEGWGADAPGENRLPHPRKGQMVP
                     RGGNQVYVDGSAEWIRFEQMFFLAGWNGSRRGCFYQDDLGDLGIFRRYIKAVP"
     gene            complement(5690..8869)
                     /locus_tag="PKI07_12125"
     CDS             complement(5690..8869)
                     /locus_tag="PKI07_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOC51831.1"
                     /translation="MKSFRIYSMAPWTRALAAALFVTAASSTQAVIVGPYAPDPDTLH
                     LWHMDVSVTPVPDEVANGLALNNAGGGATLGNASFSGFGQAVSTLDGGQAGRNEATLI
                     DAYLAPKALANGSGDNVAWSFADPTTRAFTFEAIVWVGFDPELNLGKAPAGTGRDQGL
                     QIISGEQEAAGGGIRSWQFRLLPVGYGPGGTTSPRLEFINVNNGASVQSIVAYIPTNG
                     THAILSNAWYHVAVTYNGDEGTPDNLRIYWTALDDSQVQANEILATSMALDLLAGAVD
                     FCIGNTGRNPPNSNWLGLIDEVRISKTARAADGMMFASPAPRIVTGPADQTVGVGQTA
                     EFTVTAAGATPLYYQWQWHGTNLPGATDPVLTFPAAQLTDSGPYSVTVSNAFGQATST
                     PDAILTVRVPRQLVWAGYGPYWNTDDPYWDSDNDNAPDTAFMPGDAVVLNDVGAWMSQ
                     LLVDSALNPTAVTVDSSADYTLTSVMNGTLVNKMTLTKKGAGQLVMDMDNNFVGPTVI
                     EDGRLVIGAGQSRGSLGLGPITNDATLAIARSGSLNLGGLLAGTGSLENNATNTVTLS
                     GTNLMSGPVSLNAGNLRLVSPAAKGPATAYTLNASAESAGTTTLSLGGGITFGPDITL
                     AMMGTSSSLNNRCSLRSLNGTNTLDCSMILDGDGNIQFWSDGLGDTNLFVVTTPLIDA
                     PLYTGQIILRGPGDGLITSRLNLGNKFSKTDTGTWTLTSTGNSWMQTDVASGKLRLGA
                     DHALPMSVLLNLNGGTLDLAGHTQMVGGLSGNGSVASSSTTADSFLIVQLMGNSFFGG
                     TIRDSTSGGTRTVSLAQHGGRLTLSGTNTYSGVTLVQGGTLELFVNGTIPNSTSIHLG
                     GASAIDVSLRADSTFTLNPAQAISADWDINITGQFASQGTVALQLNKTGLLLTNSQLH
                     ASGGVTYGGTLQLQVTASPALAAGDAFKLFDAGSYSGAFASIVPATPGDGLVWDDSTL
                     ATDGTLRVATPGGPVTGTNLLFAVTGAGLELSWPESYLGWRLEGQTNSPGLGLSSNWF
                     EVPGSTATNRILLSLDPALGSVFYRMVKP"
BASE COUNT         1550 a         2916 c         3231 g         1714 t
ORIGIN      
        1 aagggttaag aaatcgaagc ccgccaccgc cccagcctcc accgccgccc acaagaccgt
       61 ctcttctccg tcctttcgag tcatgtacaa ggggagtaca tcggaagccc ggcaggagca
      121 catagaaaat aagcatgaga cccctttgag cctcctttgt acccccgttg tacacggcct
      181 aatgggagcg ttaggctggc tggggcgggt ggggcgggtt tgggcgcggg cctgagccgg
      241 aagggaggga tggacgactt gcggatgatg taaaaggacg tttcaaggcg ggagggagag
      301 gacgagagcg ttcggcaatg gcagaacggg gggtcgggtg ttgtcgcgcg ccgtggtttt
      361 tgggcgggat gggatcgcga ttggcgggga cattccccta cccgggcgga ggttgccgaa
      421 ttgaggctgc ttgcgttgcc ctcatcgcgg gcggcgtatt cgcgcgattg gggcgcgaca
      481 gttgcaaagt tgcgttccgg tcagagcagt ccgcggccga aagcgtgctg ctgtgagggg
      541 ggcgcgtggt ctcccggggt tctatttttt tcggtttagg ctttacacca gcccggggcg
      601 ggacaaagtc attggcatga ggaatctgtg gcgatggatt gcgtttgcct ggttgtccgg
      661 attggcgact ctgccggctt cggcggcctt cagttcgatt tatgttctcg gagatggagt
      721 ttgcaccacg acggcgtccc cttcgccgcc gagtcttttt tacggcaatc gcttttgcaa
      781 cggccgcgtg tgggtcgagg tgctggccca gcggcagggg ctgaccttgc ccaccagcca
      841 gaatcaatcc tacttcggcc acaccagcac caacttgctg gccctggtca gcggccattc
      901 cttcacgctg ccgcccgcgg cgacgaactg gctgtttgtg gtatgggtct gtgatgcgga
      961 ttttgtcggc gatattgaaa atgtgccctt caactcgagc acctgggata aatacgtcaa
     1021 cgcttccctg accaaccatt ttcgggcggt cacgaatctc tatgccaagg gcgcgcgcac
     1081 cgtaatcatg ccgaacgcgg tggatatcac catggttccg aactactacg agcgcccgaa
     1141 caaggccttt atccggcaga aggtgattaa cttcaacacc gggctggcca gcgccgtcgc
     1201 cagccagatc aaggccaaat gccccctcat cactctttat attcccgatt ttttcgcctt
     1261 gttcgacagc atgctggcca cccccgccac ttacgggctc agcaatccca gccccgggtg
     1321 ctatgcgatt ggggatggcc acacctcgct cagcagcggg ccgggggtga gttatatctt
     1381 ctgggatgac accgatccga cggcgaaagc ccaggcgatc atggccgatg ttgcgcagca
     1441 aatgatttct ccggcgacgt tctcccgggt caccacacag ggggccagcc tccggctgga
     1501 tgtggtgaac cttccggtgg gcctgagcgg gtcggtggaa agccggtccc aggcgggcgc
     1561 gggggcctgg tcgccggtgg ccagcttcaa cagcaccaac cttacgcagg cgctttatgt
     1621 gactcccgcc ggggcgcggc agttttaccg gctgcgcttt ccctttgcct ggacctggcc
     1681 ctagacggcg cgggttgcgc cgggcggttt ccggtggccg caccgccgcg ggcgaacggc
     1741 tggggagccc gggcgcgggg cgcggcgggc gccgggttaa tcgctgccct cgtggaggcg
     1801 caggcgccag aagcgggggc tggcgctgac gccgttggta tggacgtatt gcagccgccc
     1861 ggctattccc gccagcgtgg ccagggtgtc ccagtcgctt agattcgtgc tcgtctgtac
     1921 ttggtaagtc cagccgggtt gcgcgttgaa cgccagcgtg gcgtccgcgg ttccggaccg
     1981 ggaaacctgc aggaacgggc gatgcgcgaa gtatgcggcg gcgtcccatt cgagcacccc
     2041 gatcggcgcg gcggtgttgg cgggcgcggc atagcccagg ccgttcaccg gctgataaaa
     2101 gatgtgcagc acgttatccc gctcccagcg cggcaggtcc acgttgggcg cgtcgaagcg
     2161 gcccaggttc tccgtggtca ggtcgaaggc ggtccacacc tggcgcgcgg ggtcctgggc
     2221 gcgcggcagc gagtggacca cggtgagccc gttgctgccc tcgttgtcgc ggtagatgac
     2281 gatcaggcgg tcgtcccgat cgcaaaggat ggttggccgg cccatgtcgc ccagcgcggt
     2341 ctccggcact ttgtaggcgg gcgagtcgag ggtgcgatgc gagagctggc gcgtttccca
     2401 gccggcggcg gcggggaagg ccacgaggta ctggcggcgg tggttattgg tcgccgcgcc
     2461 cggggcccac cagttggcga tcaccggctg gccggactgg tcgaggcaca ttcccgactg
     2521 gttcatcagg ctcgagccct cggggatgtc gaggaccttt tccgcgatgg tgttggggtc
     2581 gccgttctcg ccgctttccg aaatcggcag ggcgtagggc gtgccatccg agcggagcca
     2641 ggtgatgccg ccgttggccg agcgcgcata ggcgaagtcg tggttggtct ggtagccgac
     2701 ctcctttttc ggcgagtccg cgttgtagcg ccaggtccag accagcagca ggttgccggc
     2761 ggcatcgagg cagggcatct gccagtaggc gttgtaattg ggcgtccacc cggtgccttt
     2821 gatgaagggg cgcgactgga cgccgtcggt gtgaaggtta acccaactgt ggtgtatcag
     2881 ccggtagcgg ttcaggaagg tgtcgccgtt gcccgaggag cctttgcgga agagaaagag
     2941 caaatctccg ccggggagcg tgatgaattg cgggtaggtg acggcggttt cctggccggt
     3001 catggtgccg tccggcccaa aggcgatggg cccgttcccc agcaccggcg cggtgctgcg
     3061 ggcgtaatgc aaggcatcgc catgcatgcc ccagctcagg tgcatatagc cttcgccatc
     3121 aaaccccatc gagatgcagt cgtggccgtc ggcgatattg ttggcggtga aggaggtgcg
     3181 gaatacctcc cagcggttgg tggtgatcgc ccggcgcgcc acccagagcg tgttgttgaa
     3241 gctgtagttc gggttggtct gatgccgccc atagtaggtg atgaactgct gctcccccac
     3301 cgtcatcagg gaggagcgga tgaatgacgc ggtgttgatg ccgccgccgg cgtaggcgta
     3361 ctcgctggtg tcggggtcgc cgtcttcaat ggggataaag cgcacccgcg gcccgggcgg
     3421 gcaggaatgc gggtcggccg ggttggtgcc ggcgcggtat tcctgccggt tggagaagcc
     3481 gtcatcgtcg ggatcatctt ccggccccag gtccagggag ccgaaatggg cgatctccca
     3541 tgcgtccggc agtccgtcgc cgtcggtgtc ggccgggctg gatgcggcgt cggccgggtt
     3601 ggtgccggcg gcccactcct cggcgttggt gaagccgtcg ccgtcgggat cgtccgccgg
     3661 ccccagggcc agggagccga aacaggtgat ttcccacacg tccggcagcc cgtcggcgtc
     3721 cttgtccccc ggcgtggagg ccttccgcgc cggattcgag ccgccccggt attcctgcca
     3781 gttggagaag ccgtcaccgt cgggatcatc tgccggtccc cgggccagcg aaccgaaatg
     3841 cttcagctcc caggcatcgg gcaggccgtc gccgtcggtg tcggcgaaga aaatgaactc
     3901 gtccggcccg cgcgccacgc tgctgatgcg ggcctcatcc agcaggccca cccagttgcc
     3961 gtcggagccg ccggtggcgc ggccttcgtt gccgaggccc cagtccgcgc tgcccgtcgg
     4021 cagattgttg gtcatgcgca ggctggcgag cagatcggcc cgcgtgcggc ccggttccgc
     4081 ccgggtccag tagagcttga ggttgcccgc ggtattcggc cgcccgtcgt aggagacggc
     4141 cacgtgatac caattgcttg cccccagggc gttggagccg ctcatgggca gataggccat
     4201 cagggtttgg atgcccggcg gggccgcgcc gatgttgatg aattgcaact gcgggttcgg
     4261 gtcgttggtc cgggcccaga caatccggaa ttgaaagagg cggagcgttt cctcggcgtc
     4321 ggcggagagg atctgcatgg tgcgcgattt gtcgggcgcc accgcgaggt tggtggggcc
     4381 aaaatccgcg cgcaccaggg cttcgaaagt gaacgcgccg ttggtgccgg cgcagacgag
     4441 caaggcattg tcgccggggc cgtttacgaa cggcaggggg ccgagatacg cgtcccggcc
     4501 gggaacgcca ttcgggttgg cggcgagcgt ggcgccgggg ccgccgtcgt aagtgctggc
     4561 cgccgagccg aagcccggca gcgcgggatg ggcaagcgag gcgccgttgg cgatggcggt
     4621 gagcgccact ccgcccgcca ccgcattggt cgcggaggaa gcgctggccg gttcgtccag
     4681 gtgccagaga tgcagggtgt cggcgtccgg cggataaggc gccatcgccg ccgggagcga
     4741 acccgcgccg gccagcgcca gccccaggct tccccggacg aggaaacgcc ccagcgcgcg
     4801 cgcgaagcgc atttcccgcc agccgttgag atgtcgcgga gctttatggc ccccgggcac
     4861 gccgacggca ctcaagggac agccttaatg tagcggcgaa agattccgag gtcgccgagg
     4921 tcgtcctggt agaagcaccc gcgccgggag ccattccacc ccgccaggaa gaacatttgc
     4981 tcgaaccgga tccattccgc cgagccgtcc acataaacct ggttgccccc ccggggaacc
     5041 atctggccct ttcggggatg cggcagccgg ttctcgcccg gggcgtccgc gccccacccc
     5101 tccgggatgt atttcacgtt ggcctcggcc gccagcaccc agcgcggttg ggaaaggccc
     5161 attttgatgg ggcttcgtga tttgacctcc gcgccgccgg tggcgaagtt cagccacgtt
     5221 ttgacgccgc ccaggtactg gtagccgagc gcgtattggt tataccggtt gacctgcggc
     5281 agcccgggcc gcgccggaca agcccacaca ttggccgtat tggtggagtt gatcaggccg
     5341 acggacttgg ctacctcgga gttctccggg ctgatgacca gctggacaaa tttcccctgc
     5401 tgctcccagg cgggcagcag ccagtccgcg ttgtccagcg cgtaaacatt catcgccacg
     5461 gcgatttgcc ggaggttgct caggcactgg gtgcgcgtgg ctttctcctt ggcggaggcc
     5521 agcgccggca agagcagcgc cgccaggatg gcaatgatgg cgatcaccac cagcaactcg
     5581 atcagcgtaa agccgtgccg cgggaaattg ccggccgcga gcgccgccgg cgcccgtccc
     5641 ggaacgggac ggggccggcg tatcaccgga cccaccaact ttcgcatact caaggtttga
     5701 ccatgcgata gaagacgctg ccgagggcgg gatccaggct caggaggatg cggttggtcg
     5761 ccgtggaacc gggcacctca aaccagttcg agctcaggcc gagcccgggg ctgttggttt
     5821 gcccttccaa gcgccagccg aggtagctct ccggccagga caattccagc cccgcgccgg
     5881 tgacggcaaa cagcaggttg gtgcccgtga ccgggccccc tggcgtggcg acccgcagcg
     5941 tgccgtcggt tgccagggtg ctgtcatccc agaccaggcc gtcgccgggc gtcgccggca
     6001 caatgctggc aaaagcgccg ctatagctgc cggcgtcgaa cagtttgaag gcatccccgg
     6061 ccgccagcgc gggagaggcg gtgacctgca gttgcagcgt gccgccgtag gttaccccgc
     6121 cggaggcgtg gagctggctg ttcgtcagca ggaggccggt cttgttcaac tgcaacgcca
     6181 ccgtgccctg gctggcaaac tggccggtga tattgatatc ccagtcggcg ctgatcgcct
     6241 gggcgggatt gagcgtgaag gtcgaatccg cgcgcaggga tacatcaatc gcagacgcgc
     6301 cgcccaggtg aatgctggtc gagttgggaa tggtcccgtt aacaaacaac tccagcgtgc
     6361 ccccctgaac cagggtaacc ccgctgtaag tattggtgcc cgacagcgtc agcctcccgc
     6421 cgtgctgcgc caggctcacg gtgcgggtgc cgcccgaggt ggagtcgcgg atggtgccgc
     6481 cgaagaacga gttgcccatc agttgcacga tcaggaagga gtccgcggtg gtgctgctgc
     6541 tggcgaccga tccgtttccg ctcagcccgc ccaccatttg ggtgtggccg gccagatcca
     6601 gggtgccgcc attgaggttt aacaggaccg acatcggcag ggcatgatcc gcgcccaggc
     6661 ggagcttgcc gctggccacg tcggtttgca tccaggagtt gccggtcgag gtgagcgtcc
     6721 aggtgccggt atcggtcttg ctgaacttgt tgcccaggtt caaccggctc gtgatcaagc
     6781 cgtctccggg gccgcgcaga atgatctgcc ccgtatagag cggggcgtca atcagcggcg
     6841 tggtcaccac gaacaggttt gtgtccccca ggccatccga ccagaactgg atgttcccgt
     6901 cgccgtccag aatcatggag cagtcgaggg tgttggttcc gttcaaagac cgcaggctgc
     6961 accgattatt caggctcgac gaggttccca tcatggccag ggtgatgtcc ggcccaaacg
     7021 tgatcccgcc gcccaggctc agcgtggtgg ttccagccga ttccgccgag gcgttcaagg
     7081 tataggcggt ggcgggtcct ttcgccgccg gactgacgag ccggagattg cccgcgttga
     7141 gactgacggg cccgctcatg aggttggtgc cgctgagggt gaccgtgttc gtggcgttgt
     7201 tttccaggct tccggttcca gccagcaagc cgcccaggtt gagggatccg gagcgggcaa
     7261 tcgccagcgt ggcatcgttg gtgatcgggc cgaggcccag cgagccgcgg gactggccgg
     7321 cgccaattac cagccgcccg tcctcaatca ctgtggggcc gacgaagttg ttgtccatgt
     7381 ccatcaccaa ctggcccgcg cccttcttgg tcaacgtcat tttgttgacc agcgtgccgt
     7441 tcatcaccga ggtcagcgtg taatccgcgc tcgagtccac cgtaaccgcc gtcgggttca
     7501 gagcggagtc caccagcaac tgcgacatcc aggcacccac gtcattcagc accacagcgt
     7561 ctcccggcat aaaggccgta tccggcgcgt tgtcgttatc ggagtcccag taaggatcgt
     7621 ccgtattcca atacgggcca tagcccgccc aaaccagttg gcgcggaacg cgcacggtga
     7681 ggatggcgtc cggcgtggag gtcgcctgcc caaacgcgtt gctgaccgtc acggagtacg
     7741 gccccgagtc tgtcagttgc gcggcgggaa acgtcagcac gggatcggtg gccccgggca
     7801 ggttcgtgcc gtgccattgc cattgataat acaagggagt ggccccggca gcggtgacgg
     7861 taaactccgc cgtttgcccg acgcccaccg tctgatccgc cggcccggtg acgatccgcg
     7921 gcgcgggcga ggcgaacatc attccgtccg ccgctcgcgc cgtcttgctg atccgcacct
     7981 cgtcaatcaa gccgagccag ttgctattgg gcggattgcg gcccgtgttg ccgatgcaga
     8041 aatccaccgc cccggcgagg aggtccagtg ccatggacgt ggccaggatt tcattggcct
     8101 gcacctggga atcatccagc gcggtccaat agatccgcaa attatcgggg gtgccctcgt
     8161 cgccgttgta ggtgacggcc acgtggtacc aggcattgga aagaatggcg tgcgttccgt
     8221 tggtgggaat gtaggccacg atgctttgca cgctggcgcc attattgacg ttgatgaact
     8281 ccaggcgcgg gctggtggtg ccgccggggc cgtagcccac cgggagcagc cggaattgcc
     8341 aggagcggat gcccccgccg gcggcttcct gctcgccaga gatgatctgc agcccctggt
     8401 cgcgcccggt gccggccggg gccttcccca ggttcaattc cgggtcgaac ccaacccaga
     8461 cgatggcctc gaaggtgaaa gcccgggttg tcggatcggc aaagctccag gcgacattgt
     8521 ccccggagcc gttggccagc gcttttggcg ccaggtaagc gtcaatcaga gtggcctcgt
     8581 tgcggccggc ctggccgccg tccaatgtgc ttaccgcctg gccaaacccg ctgaaggagg
     8641 cattgcccag cgtagccccg ccaccggcat tgttcaacgc cagaccgttg gccacctcat
     8701 ccggcaccgg cgtgacactg acatccatgt gccaaagatg cagggtgtcc ggatcgggag
     8761 cgtatggccc gacaattaca gcctgcgtgg aagacgccgc cgtcacgaaa agtgcagcgg
     8821 ccagcgcccg cgtccagggc gccatggaat atatgcggaa tgatttcata ttgttatacc
     8881 agctgaacag ttttgtgttg ttgtcatttg tttgggcagc ccatggttgg cgccacccat
     8941 taagttgggc gcaattgtag ccatcctctt ccaaaaggca acaaaaaaat atgccaaaat
     9001 gccggcattt cagatgcgcc ccgcgaggcc ctttattttg aagcgcccgg gcaaaggcga
     9061 gcgggattct gcggcttcgg gctgggtcag ccgacggtgg cttgcggatt tgaggccagg
     9121 ccggacccgg aaaggattgg ccctgccggg cacacaagcc gcggcccggg aagacccggc
     9181 tgacccggga gttgcggcgc gcggagggga gacgcccccg cccgggagga catcgcgccg
     9241 cccacaatgg cgggtttcgg cttcgtggcc ttgaccgcga atgaactcgc cgcccgggtc
     9301 cgggcggcca ggatccgcgc cgcgcgggcg tggcatccgg ccggccgcgc gggcaaacgg
     9361 gtagtgtcct aaaagttctg caaaaagcag cgggtcatgg taagccactg g
//
LOCUS       DAOPQG010000129         9344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_8331_length_9344_cov_14.671224, whole genome shotgun sequence.
ACCESSION   DAOPQG010000129 DAOPQG010000000
VERSION     DAOPQG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8331_length_9344_cov_14.671224"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            523..1263
                     /locus_tag="PKI07_12130"
     CDS             523..1263
                     /locus_tag="PKI07_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOC51832.1"
                     /translation="MVRLDQALVERGFCPSREKAKRAIMAGQVRINDRPGRKPSDSVQ
                     PADQLSVLAPEKYVSRGGYKLEHALEHFQVDVAGQVAVDLGAATGGFTDCLLQRGAAR
                     VYAVDVGQGQFAWKLRHDPRVVLLERTNARDLAPAKLPPPFAAAHLVAIDCSFISLRK
                     ILPAAVSLLGPGGKIVALIKPQFEAGKTEADRGAGVIRDAAIHERVVRELQEFAAARL
                     GARWRGVTESPLLGPAGNKEFLALMETP"
     gene            1260..2144
                     /locus_tag="PKI07_12135"
     CDS             1260..2144
                     /locus_tag="PKI07_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOC51833.1"
                     /translation="MKPSGDRIQRVGLIGNARKAASGVTVQRAARLIAAAGRQVCTDA
                     ATARSVRLAAAVYPDPAALARQVDLLMVFGGDGTMLRAVREIAGSRTPVLGVNVGGLG
                     FLTAVPSARLAAALRELWAGQFNFEARALLQASGHCDGQWQEATALNDIVISRGIASR
                     LIELDVSVDGAPLTRYRCDGLIVSSPTGSTAYSLASGGAVVFPTAEVFALTPICPHTL
                     SNRSLILPLTATMEVKVISPRPATILSSDGEVVSELAAGDRITIRRSRRTVRLLHLAG
                     SSFCETLRCKLHWRGASL"
     gene            2141..3175
                     /locus_tag="PKI07_12140"
     CDS             2141..3175
                     /locus_tag="PKI07_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC51834.1"
                     /translation="MTRLRDIARQAGVAANTVSKALRDAPDLSPATKARIRLLAQQLG
                     YVPDSAARTLRTRRTKLFGLVISSMTNPIYSRVVLAIEEGAHEMGYDVLFAHTLNIPE
                     REEACIRQMLSRRVDGLFIAPVYRLASEVRIYQEVLARRVPTVVLGHTTPFCNQFINV
                     ETDDLQASYTVTRHLLKLGHRRIAFLGGPPATPWTQERLEGYRQALREANLEEDDKLI
                     FTAGRTLEEGAAAARQILAEDSDITAVQAVNDLVAAGCAEAFLEEGFQIPRDVSIAGF
                     GNILLGAHFRTPLTTTRQPKFRLGRAAVRTMRELLLGRQPESSRLPAELIVRASTGTP
                     PAARQLRLLK"
     gene            3195..3617
                     /locus_tag="PKI07_12145"
     CDS             3195..3617
                     /locus_tag="PKI07_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51835.1"
                     /translation="MRKALAIALVGLGVGGVIWVAKGWALKGPESKTARFNAEADNLI
                     LGLQQYREFVGSYPAGNNIAIARALLGQTDKKVLILAVRKSDLNEKGEILDPWGTPVR
                     FFFSENEVMIRSAGPNRIWEDSAVSNSDDLYRTAPMER"
     gene            3776..4816
                     /locus_tag="PKI07_12150"
     CDS             3776..4816
                     /locus_tag="PKI07_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC51836.1"
                     /translation="MAKQHQYQFCFGPWNISEGQDPYGPPTRPAQTFDWKLKQLKKSG
                     FDAMMFHDDDAVPDIDGKSDAQVRKEARELKQKLADAGVVAEMVAPRLWFSPKTIDGA
                     YTSNDPKSRQYAIERTLRCIDVANYLGTKLIVLWLAREGTYLREAKDGRRSIEWLVQA
                     LDKMLAYDRSIRISIEPKPNEPMDHAYVPTIGHALALAQLTRDPKRVGCLIESAHAIL
                     AGLDPADEIDFAMAFGKLWSLHLNDQNGLKFDQDKPFGSNNLRVAFNQVRALERGGYG
                     KRGEYVCFDVHPFRTTKVEHWLAHLVNSRRTFLRLVQKARTFDEKAARQLVADRNHAA
                     LDQMVLEHLMGK"
     gene            4891..5976
                     /gene="trpD"
                     /locus_tag="PKI07_12155"
     CDS             4891..5976
                     /gene="trpD"
                     /locus_tag="PKI07_12155"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOC51837.1"
                     /translation="MLSELTRQLAAARALTDAEVRQAVAVLVDEAVPAEPKAEFLIQL
                     ARKGETVAEITAFARELRRRSRQPVLGADLRDRPLLDVVGTGGDRLSTFNISTTVGLI
                     AAAAGVRVVKHGNRASTSLVGSADVMESLGLPFDLEPEAAVERLRAQGFAFFFAPRYH
                     PAFRHIVPARKLCAARDQSTIFNFLGPLLNPAAPTAALIGVPRPDLCEPLARVLQSLG
                     VRRAMVVCGAVADGGSAVRYLDELSPLGPTAVAEFSPGTAPVCATVAPEEFPLQPAVL
                     ADLRGGDREVNAAIIRRVLSGEERGPKRDAVLLNAAAALFVADRARSLAEGWELAAQT
                     IDSGQAAAKLAELQQPPAGERAGARAP"
     gene            6097..6240
                     /locus_tag="PKI07_12160"
                     /pseudo
     CDS             6097..6240
                     /locus_tag="PKI07_12160"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
     gene            6280..6555
                     /locus_tag="PKI07_12165"
     CDS             6280..6555
                     /locus_tag="PKI07_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOC51838.1"
                     /translation="MGTPPPYASAPSAASKIPAGICGILLGSLGIHKFILGYQDAGLI
                     MLLVTVLTCGVGACVMGVIGLIEGIIYLCKSDEEFVRIYVTGRREWF"
     gene            6583..7095
                     /locus_tag="PKI07_12170"
     CDS             6583..7095
                     /locus_tag="PKI07_12170"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOC51839.1"
                     /translation="MYQIIGSDGRTYGPATAEQLREWIAEGRADAQTQVWTEGASQWR
                     PLAEYAEFAPLLAMRPPSLAAPGTACSAARPPSPLLATASLVMGLLSLPCGLCFPCVL
                     CFPCGLCCCCGLPFNLLGIVFALVALAQMRHQPWPRAGRGQAIAGLVLSLLSLALAAF
                     WGVLFCLAAR"
     gene            7135..7557
                     /locus_tag="PKI07_12175"
     CDS             7135..7557
                     /locus_tag="PKI07_12175"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOC51840.1"
                     /translation="MSEPANSSREVWRVGGALTALVVVVAGAMLFWFDPSEFHFYPVC
                     FFHRATGLLCPGCGGLRALHQLLHGHVAAAFRFNPMLVLSLPLAAGLALRYGRPKAWR
                     QPGWSGPRSGWLWLLLAVWLAVGLLRNMPGALFAMLRP"
     gene            7638..9002
                     /locus_tag="PKI07_12180"
     CDS             7638..9002
                     /locus_tag="PKI07_12180"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOC51841.1"
                     /translation="MMYKIIGADQKEYGPVSAEQLRQWLAEGRANAQTPVQAAGTAGW
                     KPLAAYPEFAALLPADALAAATPPGYDAPRELPEDIFARDYDLDIGGCLGRAWELLKG
                     NFALVAGGVAVYFLCQIAIIGLALLCQILFSRLTHTPFVGIAGNLVNLIITGPLTGGV
                     LYFLLKSPLTGGVLYFLLRNIRRQPTGISDVFAGFRRAFGQLLLGYVVMIILIYLAML
                     PGVALMVWPLLTLARQQAVAAGPIFVALLGFICLIVPAIYLSVSWTFLLPLIIDRQMK
                     FGPAMKASRRMVGKHWWQVFGLVVVCGLINVVGFLFCGVGIFLTLPISLGAILYAYES
                     IFSPPAAPPLNRAAARNATLLNLCATPGLGSLIAGRRAAGLGQLLLAIAGFGAVCGWL
                     VLLAWQIYNAATRGAELRPTSWLGGAGAALFALAWIWSLFTSLSLLREARANEPGAGR
                     PPPL"
     gene            complement(9037..>9344)
                     /locus_tag="PKI07_12185"
     CDS             complement(9037..>9344)
                     /locus_tag="PKI07_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOC51842.1"
                     /translation="KAVRNGKLCYYINDGVYHTFSGVLFDHCQYPLQAFKGGTPQICS
                     VFGPTCDALDVVSLAEHLPANLELGDLLYSKNIGAYSHASSTWFNGFPPAKVVHINQ"
BASE COUNT         1422 a         2848 c         3305 g         1769 t
ORIGIN      
        1 atggaaaata agcatgagac ccctttgagc ctcctttgta cccccgttgt acacgaccta
       61 atgggagcgt taggctggct ggggcgggtg gggcgggttt gggcgcgggc ctgagccgga
      121 ttggaggaat ggacgagtcg cggacgatgt aaaaggacgt ttcaaggcgg gagggagagg
      181 acgagagcgt tcggcatgtc cggaacgagg ggctgggtgt tgtcgcgtgc cgtggttttt
      241 cggcgggatg gggccacggc tggcggggaa atttccccac ccgggcggga gccgccgcaa
      301 ggcgactgct tacgcggtcc tctgggttag gcggccactt tgaattgata gacgcttttg
      361 ccccagccca cccaatccgg aaagcggctt tgggtttggg gaaatcgttt cccccgcagc
      421 tatcgcaacc cgggcgccga taacccaagg ctctgccggg gagcgggggg ggcgctgaat
      481 tattctcgca acggggcggc cggccagtgc atactggggg gcatggtgcg cctggatcaa
      541 gcactggtcg agcgtggttt ctgcccgagc cgggagaaag ccaagcgcgc gatcatggcg
      601 gggcaagtcc ggattaacga tcgtcccgga cgcaagccca gcgattccgt tcagcccgcc
      661 gaccagcttt ccgtgctcgc gccggagaaa tacgtcagcc gcggcggcta caagctggaa
      721 cacgcgctgg agcattttca ggtggacgtg gccgggcagg tggccgttga tttgggcgcg
      781 gccaccggcg gttttaccga ctgcctgttg cagcgcggcg cggcgcgggt gtatgccgtg
      841 gatgtcggcc aggggcagtt cgcctggaaa ttgcggcacg acccgcgggt ggtgctcctg
      901 gagcggacga atgcgcgcga tttggcgccg gcgaagctcc cgccgccgtt tgccgccgcg
      961 cacctggtgg cgattgattg ctccttcatt tccctgcgga agattttgcc ggcggcggtg
     1021 tccctgctcg ggccgggcgg caaaatagtg gcgctgatca agccgcaatt cgaggccggc
     1081 aagacggagg cggaccgggg cgccggggtg atccgcgacg cggccattca cgagcgcgtc
     1141 gtgcgcgagc tgcaggagtt tgcggcggcc cgcctgggcg cgcgctggcg cggcgtcacc
     1201 gaatcgcccc tgctggggcc ggcgggcaac aaggagtttt tggcgttgat ggaaacccca
     1261 tgaaaccgtc cggagacagg attcagcgtg tcggcctgat tgggaacgcc cggaaggccg
     1321 ccagcggcgt caccgtgcag cgggcggcgc ggctgattgc cgccgccggc cggcaggttt
     1381 gcaccgacgc ggcgaccgcc cgcagcgtcc gcctggccgc ggccgtttat cccgacccgg
     1441 cggccctggc gcgccaggtg gatttgctga tggtctttgg cggggacggg accatgctcc
     1501 gggcggtgcg ggagattgcc ggctcgcgca cccccgtgct cggggtcaac gtgggcggcc
     1561 tgggcttcct gaccgccgtg ccgtcggcgc gcctggccgc ggcgctgcgg gagctgtggg
     1621 cgggccagtt taattttgaa gcgcgcgcgc tgctccaggc gtccggccac tgcgacgggc
     1681 aatggcagga ggccaccgcc cttaatgaca ttgtcattag ccgcggcatt gcctcccgcc
     1741 tgatcgagct ggatgtcagc gtggacggcg cgccgcttac gcgctaccgc tgcgacggcc
     1801 tcatcgtcag ctcgccgacc ggctccaccg cgtattcgct ggcgtccggg ggggccgtgg
     1861 ttttccccac cgccgaggtg tttgccctca ccccgatctg cccgcacacg ctgtccaacc
     1921 gttccctgat cctgccctta accgcgacca tggaggtcaa agtcatcagt cccaggccgg
     1981 cgaccatttt gagctcggat ggggaggtgg tcagcgaatt ggcggccggc gaccggatta
     2041 ccatccggcg cagccggcgg acggtgcgtt tgctgcacct ggccgggagt tcgttttgcg
     2101 aaaccctgcg ctgcaaactg cattggcggg gggccagcct gtgacgcggc ttcgcgatat
     2161 tgcgcggcag gcgggcgtgg cggccaacac cgtctccaag gccctgcggg atgcgccgga
     2221 tctttccccg gcgaccaagg cgcgcatcag gcttctggcg cagcagttgg gttatgtgcc
     2281 ggattccgca gcccggaccc tgcgcacgcg ccgcaccaag ctcttcgggc tggtcatttc
     2341 gtccatgacc aaccccattt attcccgcgt ggtcctcgcc atcgaggagg gcgcgcacga
     2401 gatggggtat gatgtgctgt ttgcccatac cctcaacatc ccggagcggg aggaggcctg
     2461 catccggcag atgctttccc ggcgggtgga cggcttgttt atcgccccgg tgtatcgcct
     2521 ggccagcgaa gtgcgcattt accaggaggt gctggcccgc cgcgtgccca ccgtcgtgct
     2581 cggccacacc acgccctttt gcaaccagtt tatcaacgtc gagacggacg acctgcaggc
     2641 cagctacacc gtcacccgcc atttgctcaa gctggggcat cgccgcattg ccttcctggg
     2701 cgggccgccc gccacgccct ggacgcagga acgcctcgag ggctaccgcc aggccttgcg
     2761 ggaagccaac ctggaagagg atgacaaact catcttcacc gcgggccgca cccttgaaga
     2821 aggcgccgcc gccgcgcggc aaatcctggc cgaagactcc gacatcaccg cggtgcaggc
     2881 ggtgaacgac ctcgtggcgg cgggctgcgc ggaggcgttt ctggaggagg gcttccaaat
     2941 cccccgggat gtctcgatcg ccgggttcgg gaacattctg ctcggggcgc atttccgcac
     3001 gccgctgacg acgacccggc agcccaagtt ccgcctgggc cgcgcggcgg tgcgcaccat
     3061 gcgcgagttg ctgctcggcc gccagcctga atccagccga ttgcccgccg aactcattgt
     3121 ccgcgccagt actggcactc cgcctgctgc ccgtcaactc agactgctga aataacagac
     3181 tgagattcaa tcgcatgaga aaggcgttag ccattgcgct cgtggggttg ggcgtcggcg
     3241 gcgtcatttg ggtggccaaa ggctgggccc tcaaaggccc cgagtccaaa accgccagat
     3301 ttaacgcgga agcggataac ctgatcctgg ggctccagca ataccgggaa tttgtcggca
     3361 gctatccggc cggcaacaat atcgccatcg ccagggccct gctgggccag accgacaaga
     3421 aagtgctgat tctcgccgtg cgcaaaagcg acctgaacga gaagggcgaa atcctggatc
     3481 cctggggcac cccggtgcgg ttcttctttt cggaaaacga agtcatgatt cgctccgccg
     3541 gccccaaccg gatctgggag gacagcgccg tttcaaactc cgacgacctt taccgaaccg
     3601 cgccgatgga acggtgacgg gccggcccgc gccgcatcct ttcgccttgc cccgccgccg
     3661 ccgcgggccc gggaacggcc gtttcccggc cgggaggcgc cctgctcgat cgagcgcgcc
     3721 gggatatttt tgggctttgc atttccacgg atggcgttac acttaaagga cttttatggc
     3781 aaaacaacac caataccagt tctgtttcgg tccctggaat atcagcgaag gtcaggatcc
     3841 ctacggcccc ccgacccggc ctgcgcagac gttcgattgg aaactcaagc aactcaagaa
     3901 gtcgggcttt gacgccatga tgtttcatga cgacgacgcg gtgcctgata ttgacgggaa
     3961 gtccgacgcg caagtgcgca aggaagccag ggagctgaag caaaagctgg cggacgccgg
     4021 cgtggtggcg gaaatggtcg cgccgcgatt gtggttcagc cccaagacga ttgacggcgc
     4081 ctatacgagc aacgatccga agagccggca gtatgccatt gagcgcacgc tgcgctgcat
     4141 tgacgtggcc aattacctgg gcaccaagct gattgtgctc tggctggcgc gcgaggggac
     4201 gtatctgcgc gaggccaagg acgggcggcg gagcattgaa tggctcgttc aggccctgga
     4261 caaaatgctg gcgtatgacc ggtccatccg catttccatc gagcccaagc ccaatgagcc
     4321 gatggatcac gcgtacgtgc cgacgattgg gcatgcgctg gccctggcgc agttgacgcg
     4381 cgatcccaag cgggtgggct gcctgattga atcggcgcac gcgatcctgg cggggctgga
     4441 tccggcggat gaaattgatt ttgcgatggc cttcggcaag ctctggtcgc tgcacctgaa
     4501 tgaccagaac gggctcaagt tcgaccagga caagccgttt ggcagcaaca accttcgcgt
     4561 ggcgttcaac caggttcgcg ccctggagcg gggcggctac ggcaagcgcg gcgaatacgt
     4621 ctgcttcgac gtgcatccgt tccgcaccac caaggtcgag cactggctgg cgcacctggt
     4681 caacagccgc cgcacgttcc tgcggctggt gcagaaggcg cgcacgtttg acgagaaggc
     4741 cgcccggcag ttggtggccg accgcaacca cgccgcgctc gaccagatgg tgctcgaaca
     4801 cctgatgggc aaataacgcc ggcgtccagc cgcgtttgcc cggcgcgggt ttccggcgcg
     4861 gcgggcggcg cggcgccggt gggcgcgctt gtgctttccg aactcacccg ccagctggct
     4921 gccgcccggg ccttgacgga cgccgaggtc cgccaggcgg tggcggtgct ggtggacgaa
     4981 gcggtgccgg cggagccgaa ggccgagttc ctgatccaac tggcccgcaa aggggagacg
     5041 gtcgcggaga ttaccgcgtt tgcccgcgag ctgcgccgcc gctcgcggca gccggtcctg
     5101 ggggcggacc tgcgcgaccg gccgctgctg gacgtggtgg gcacgggcgg cgaccggttg
     5161 agcacgttta atatttcgac gaccgtgggc ctgatcgcgg cggcggcggg cgtgcgcgtg
     5221 gtcaagcatg gcaaccgggc cagcacgtcg ctcgtcggca gcgcggatgt catggagtcg
     5281 ctcggcctgc cgtttgacct ggagccggag gccgcggtgg agcggctccg cgcgcagggc
     5341 tttgccttct ttttcgcgcc ccgctaccac cccgccttcc ggcatattgt cccggcgcgc
     5401 aaactctgcg ccgcccggga ccagagcacg atctttaact tcctggggcc gctgttgaac
     5461 cccgccgcgc cgacggcggc gctcatcggg gttccgcggc cggatttgtg cgagccgctg
     5521 gcgcgggtgc tccagtcgct cggcgtgcgc cgggccatgg tcgtttgcgg cgcggtggcg
     5581 gacgggggca gcgcggtccg ctacctggac gagctttcgc cgctgggccc caccgcggtg
     5641 gcggagtttt ccccgggcac ggcgccggtc tgcgcgacgg tggcgcccga ggaatttccg
     5701 ctgcagccgg cggtgctggc ggacttgcgc ggcggcgacc gggaggtcaa cgcggcgatc
     5761 attcgccgcg tgttgagcgg ggaagagcgg ggcccgaagc gggacgcggt tttgctgaat
     5821 gccgccgcgg cgttgttcgt ggccgaccgg gcgcgttcgc tggcggaagg ctgggagctg
     5881 gcggcgcaaa cgattgacag cgggcaggcg gcggcgaagc tggcggaatt gcagcagcct
     5941 cccgccgggg agcgggccgg cgcgcgcgcg ccctgatgtt ggggcggccc ggacgcgcag
     6001 ggagcgcgcg cggcgaggcg ttcccccggc ggcggattac aaaaccaggc aatttttgca
     6061 cttggcgagg ttccccggct ggtataaaag gacggcatgt ataaaatcat cggcaaggat
     6121 ggacagacct acggcccggc cagtgtggag caactgcgga tttggatggc cgagaatcgc
     6181 gtgaacgccc agaccctcgc gcaggccgaa ggcgcgccgg actggaagcc ggtgggtgaa
     6241 tggccggagc tggcggcgca cttgaaaccg cccccggcga tggggacgcc gccgccttat
     6301 gcgtccgcgc cgtcggcggc cagcaagatt ccggccggca tctgcggcat cctgctgggc
     6361 tcgctgggga ttcacaaatt catcctgggc taccaggacg cgggattgat catgctgctg
     6421 gtcaccgtgc tgacgtgcgg cgttggcgcc tgcgtcatgg gggttattgg tttaatcgag
     6481 gggattattt atttgtgcaa gtccgatgaa gagttcgtgc ggatttatgt gaccgggcgg
     6541 agggagtggt tctaagctgc gctgcctggg cggcggtgat ttatgtatca gatcattgga
     6601 tcggatggca ggacgtacgg gccggcgacg gcagagcaac tgcgggagtg gatagccgaa
     6661 ggccgggccg atgcgcaaac ccaggtctgg acggagggcg cgagtcaatg gcgtccgctg
     6721 gcggagtacg cggagtttgc cccgctgctg gcgatgcggc cgccgtcgct ggccgcgccg
     6781 gggacggctt gttccgccgc gcggcccccg tctcctttgc tggccacggc cagcctggtg
     6841 atggggcttc tctcgctccc ctgcggtttg tgtttcccct gcgttttgtg tttcccctgc
     6901 ggtttgtgtt gttgctgtgg gctgcctttt aacctgttgg ggatcgtgtt tgcgctggtg
     6961 gccctggcgc aaatgcggca tcaaccgtgg ccgcgcgcgg gccgggggca ggccattgcg
     7021 gggctggtgc tttccctttt gagcctggcg ctggcggcat tttggggggt gcttttttgc
     7081 ttggctgcgc gatgaacgcc tccgaaatca tgcggcgcct ggattgctgg tgatatgtcc
     7141 gaaccggcaa actcttcccg ggaggtttgg cgcgtcggcg gggcgttgac cgctttggtg
     7201 gttgtcgtgg ctggcgcgat gttgttctgg tttgacccga gcgagtttca cttctacccg
     7261 gtgtgttttt tccatcgcgc caccgggctg ctttgccccg gttgcggcgg cttgcgcgcg
     7321 ctgcaccaac tgctccacgg gcatgtggcg gcggcgtttc gttttaaccc gatgctggtt
     7381 ttgtccttgc ccctggcggc gggcctggcc ctccggtatg ggcggccaaa ggcgtggcgg
     7441 cagccggggt ggagcgggcc gcgttcgggc tggctctggc tgttgctggc ggtgtggctg
     7501 gcggtcggcc tgctgcggaa tatgccgggc gcgctgtttg cgatgctgcg gccatgaatc
     7561 cctggtcgcg aaaagctggc ggcccgcccg gctttcgatt aaaaccagcg taaccagtcc
     7621 atcacgaaag gaacgaaatg atgtataaaa tcatcggcgc ggaccaaaag gaatatggcc
     7681 ccgtttcagc cgagcagttg cgtcagtggc ttgcggaagg gcgcgccaac gcgcaaacac
     7741 cggtgcaggc ggcggggacg gcagggtgga agcctctggc cgcatatcct gaatttgcgg
     7801 cgctgttgcc cgcggacgcc ctggcggcag ccacgccccc gggttatgac gccccccgcg
     7861 agctgccgga agatattttt gcgcgcgact acgacctgga catcgggggt tgtctcggtc
     7921 gcgcgtggga gttgctgaaa ggaaacttcg ccctggtggc gggcggcgtt gccgtttatt
     7981 ttctctgcca gatagccata atcgggctgg cgctgctctg ccagatactc ttcagccggc
     8041 tgacgcatac acccttcgtg gggatcgcag gcaacctggt aaatctcatt atcaccggtc
     8101 cgctgacggg gggcgttctc tactttttgc tgaagagtcc gctgacgggg ggcgttctct
     8161 actttttgct gaggaatatc cggcggcaac caacagggat cagcgatgtc tttgccgggt
     8221 tccgccgggc gtttggccaa ctgctgctgg gctatgtggt aatgataatc ctgatttacc
     8281 tggcaatgct gcccggggtg gcgctgatgg tctggccgct actgaccctg gcgcgccagc
     8341 aggccgttgc ggcggggcca atcttcgtgg ctttgttggg ctttatctgt ctgatagtcc
     8401 cggccattta tctgtcggtc agttggacgt ttttgctgcc gctcatcatt gaccggcaaa
     8461 tgaagtttgg gccggcgatg aaggccagcc ggcgaatggt gggcaagcat tggtggcagg
     8521 tctttgggct tgtcgtggtg tgcgggctga tcaatgtagt tgggttcctg ttctgcggcg
     8581 tgggcatctt tttaacgctg cccatctctc tgggcgcgat cctgtatgcg tatgagagca
     8641 tcttttcccc gcccgccgcg ccgccgctta accgcgctgc cgcccgcaac gccacgctgc
     8701 ttaatctgtg cgccacgccg gggttgggct cgctcattgc cgggcggcgc gcggcgggcc
     8761 tggggcagct tcttctggcg attgccggct ttggggcggt ctgtggctgg ttggtgctgc
     8821 tggcctggca aatctataac gcggccaccc ggggcgccga acttcggccc accagttggc
     8881 tgggcggcgc gggggcggcc ctcttcgccc tggcctggat ttggtcgttg tttaccagcc
     8941 tcagcctttt gcgcgaggcg cgcgccaatg agccgggcgc cgggcggccg ccgccgcttt
     9001 gaggcggtgc ggggggatgg cccccggcgc gggcgcttac tggttgatgt gcaccacttt
     9061 ggccggcggg aagccgttga accaggtgga ggaggcgtgg ctgtaggcgc cgatgttttt
     9121 gctgtagagc aggtcgccga gttcgaggtt ggcgggaaga tgctcggcca gcgagaccac
     9181 gtccagggcg tcgcaggtgg ggccaaacac cgagcagatc tggggcgtgc ctcctttgaa
     9241 ggcctggagc gggtattggc agtgatcgaa gaggacgccg gagaacgtat gatagacccc
     9301 gtcgttgatg tagtagcaga gcttgccgtt gcgcaccgcc ttgc
//
LOCUS       DAOPQG010000130         9312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_8362_length_9312_cov_14.451766, whole genome shotgun sequence.
ACCESSION   DAOPQG010000130 DAOPQG010000000
VERSION     DAOPQG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8362_length_9312_cov_14.451766"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..860)
                     /locus_tag="PKI07_12190"
     CDS             complement(231..860)
                     /locus_tag="PKI07_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51843.1"
                     /translation="MKVLPWLCVVGLLAGLGWVYSASQKKDAALAALRAEHQQLQQER
                     AALEEAAQARTEAENNELLRLRKDHEELLRLRNEVRQLRADKDQLGAQVRSAQAQAQG
                     AQEQLQALRTSAAQPAPPAPGAPAAPATPEQQQAQLCIHNLRLIHAAKQQWAQQRQKP
                     SGTLITPADIAPLLPNQTVPSSCPAGGVYTLNPIGVPPICNIPGHSLAK"
     gene            complement(882..>1184)
                     /locus_tag="PKI07_12195"
     CDS             complement(882..>1184)
                     /locus_tag="PKI07_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOC51844.1"
                     /translation="FVRHKERKFGRGCLEQWRRQRKELLAQFTQRLEPFEKMLWNRPF
                     LLAHEPRFVDFDLFGILGNFLYSGHYRLPPAHPRLRKWHARMAAAQHSAGNDKVPF"
     assembly_gap    1185..1284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1544..2938
                     /gene="mpl"
                     /locus_tag="PKI07_12200"
     CDS             1544..2938
                     /gene="mpl"
                     /locus_tag="PKI07_12200"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HOC51845.1"
                     /translation="MFSRIQSVHFIGIGGTAMASVAAALREKGVKVTGSDQNIYPPMS
                     TFLAERQIEVNPGYAEPNLAHRPDLVVIGNAISRGNPEAEYVLDHKLPYCSLPELLKY
                     YFLRGKRSVVVAGTHGKTTVTALLAWVFEHNGLNPSYLIGGIPNNFSQGARFTDSEWF
                     ILEGDEYDTAFFDKRSKFVHYLPEVAIINNLEFDHADIFDNLEAIQTAFKYFIRLIPR
                     NGLLLGNGDDPRLAPLLDVTHCPVKRFGLGEINAVQAFNLRYGPTASEFEIPSFKFRT
                     NFLGEINVRNALAVVACAKHCGLKNQQIQSAFDTFKGIKRRLEVRGLAGGVIVLDDFG
                     HHPTAIRATLSALRQKYPTQRVWAIFEPRTNTTRRRVFQAELAGAFAEADAVVIAQVD
                     RLELLAPEERLDPERLMRDLRDAGKEAVYLPDADAIAAHVAQQVQGGEVVIVFSNGGF
                     GGLHGKLLARLGRG"
     gene            3076..3792
                     /locus_tag="PKI07_12205"
     CDS             3076..3792
                     /locus_tag="PKI07_12205"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51846.1"
                     /translation="MRKNRRTWQSFEEVRKAADQGDAAAQCYLGICYQTGQGVPQDYA
                     EAVKWFRRAADQNDDTAQCYLGFCYQSGLGVPQEYGQAAKWFREAAERGDPAAQFNLG
                     VLYETGQGVPQNYAEAVKWYHAAAEQGEPQAQFNLGVFYENGQVVPQNYEEAVKWYRS
                     AAEQECAPAQCNLGLCYETGRGVPKSVREAVKWFCRAARAGDKTAQHNLGVYYASLEA
                     AEAAASKDEPPDAAPRGKAG"
     gene            3810..4775
                     /locus_tag="PKI07_12210"
     CDS             3810..4775
                     /locus_tag="PKI07_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC51847.1"
                     /translation="MNKGFRTNSRARSAEPALIRFWGVRGSIPAPGPGTVRYGGNTSC
                     VEIRADGQIIILDAGTGLRLLGHHLLAEAKEQPLELTLLLTHTHWDHIQGLPFFSPVY
                     QPQNHLRVLGYEGARHGLAVVLAGQMATPFFPVRLREVPANVRIRELKQRRFQIGPVR
                     VQARPANHPGKCVGYRLFTSCGSVAFFPDHEVPVANDAGAPGRIPAPAANRAMIQFLH
                     GADVLIMDTQFDAEEYRQHRGWGHGCMDAVVELALQAKVKTLFLFHHAPDHDDAKVAR
                     MLAHARRIVARRKGSLRVNAAREGMTVKLPPAERVGGGADAATNG"
     gene            complement(4784..5509)
                     /locus_tag="PKI07_12215"
     CDS             complement(4784..5509)
                     /locus_tag="PKI07_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51848.1"
                     /translation="MTNSGKLLLCVLAALVSWPAIRAAGNETSGGNPYQAIVDRNVFG
                     LKPPPPPPDPEANKPPPPKILLTGIYEMGGTKRVLMKVSMPAKPPEPAKEIPLTLSER
                     QRDGEIEVLEIDTVARTAKVNNHGTITSLDFTNNGVKVAATPAPGKPAAPTPPTRTPT
                     THPGAVKRNWPPEPTTPEEAAIREAAYMMRNKAAIDRGELPPLPGNNPLLQNQPAPTH
                     APVQMQPPPMPPLPPGAARASPY"
     gene            6077..6283
                     /locus_tag="PKI07_12220"
     CDS             6077..6283
                     /locus_tag="PKI07_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51849.1"
                     /translation="MNKTTRRIVKSDRTGKTTPRANGEVICRGELWRATDSVVNALGM
                     LWCAKPAEKFVVPPTHDCSGDLPD"
     gene            6425..6997
                     /locus_tag="PKI07_12225"
     CDS             6425..6997
                     /locus_tag="PKI07_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC51850.1"
                     /translation="MSRVVSEPLAEREGPDAPQAGLAPSAFADLYTRHSRAIYYLCLR
                     LLNDPGKAEDATHDVFLKAFCKRNQFRGESSWRTWLYRIAINHCRNLLAAWHERHMLS
                     NADEALWENTAARTDSPLRVVEIRELGERIQQALVGLPGEYRLLLLLVADEELSYEQV
                     GALTGQSADAVRGKLHRARKAFIVRFRQTE"
     gene            7007..7237
                     /locus_tag="PKI07_12230"
     CDS             7007..7237
                     /locus_tag="PKI07_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51851.1"
                     /translation="MKRQTKLTAQEQEQLDARQQQAAAAAPREFASVEEMLRHDAQCT
                     PVPPAIARRLEKSVRDLPPPPARAWWRRFFGS"
     gene            7262..8809
                     /locus_tag="PKI07_12235"
     CDS             7262..8809
                     /locus_tag="PKI07_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC51852.1"
                     /translation="MSFSDEVEILIRARYPILYIITSEELRVEKILRQIAAGRQKKVF
                     EWTCSSGVVAAGTPFQSQKGRNPATREPLAALDLVVEQLEPALFLFKDFHPFLARGNF
                     AIIRRLKDIALHLKNSRKTIVLISPVLEIPPELDKEITVLNFPQPGKDELGELLDKAA
                     AELRESGEVPVELDEPGRDRLLQAALGLTLGEAENVFAKIIVKNQRLSEDAVGEVIAE
                     KQQIIRKSGLLEYYEAEEDFDHVGGLDVLKDWINKRAAAFTDEARAFGLPAPRGVLLL
                     GAQGCGKSLCAKAVSRRWQLPLLRFDMGRMFSSLVGSSEENIRRAIAVAESIAPVVLW
                     VDEIDKAFSGSQSSGFTDGGTTARVFGTFLTWLSEKRAPVFVVATANDVSQLPPELLR
                     KGRLDEIFYVDLPRPPERADIFRIHLAKRHRHPGNFDLPALVAASPEFSGAEIEAAII
                     SALYDAFYAQQELTTAHVLEALHQTVPLARTLAEKISEQRAWAAGRARNASIASSVRD
                     LAEARVP"
     gene            complement(8899..9186)
                     /gene="gatC"
                     /locus_tag="PKI07_12240"
     CDS             complement(8899..9186)
                     /gene="gatC"
                     /locus_tag="PKI07_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOC51853.1"
                     /translation="MNTAEFDVKHIAHLARLSLSPEEEQTLGGQMGNILGYIEKLKEV
                     DVSGVDPTAHAFPLVNVARPDEIRPSLSNEEALRNAPARANGLFIVPRIVE"
BASE COUNT         1661 a         2824 c         3016 g         1711 t
ORIGIN      
        1 ccggcgcgat ctcgcgcggg aatcacgcgc cgtccgggaa agcccgggcg gcgcgatttg
       61 ttttcggttt cgtcactggc gccgggcggg ctgaagcgcc gggggcgatt gcgcaagcgg
      121 ctttccgtgc gccctgatcg gggcgcctcg agtcggggcg gttccgcggc gcggccgctg
      181 gcggccagct cgagggcagg cgagggcggg gccgcgccag ccgggaatgc ttacttcgcc
      241 agggagtggc cggggatgtt gcagatgggc gggacgccga tggggttcaa cgtgtaaacc
      301 ccgcccgccg ggcaggagga gggcacggtc tggttgggaa gcagcggcgc gatatccgcc
      361 ggggtgatca gggtgccgga cggtttttgc cgttgctggg cccactgttg tttggcggcg
      421 tggatgagcc ggagattgtg gatgcacaac tgggcctgtt gctgctcggg agtggcgggc
      481 gccgccgggg ctcccggagc gggcggcgcc ggttgcgccg cgctggtgcg cagggcctgg
      541 agctgttcct gggctccctg ggcctgcgcc tgggcggatc ggacctgcgc gccgagctgg
      601 tccttgtcgg cgcgcaactg gcggacctcg ttgcgcaggc gcagcagttc ctcatgatcc
      661 ttgcgcaggc gcagcaactc gttgttttcc gcttcggtgc gcgcctgggc ggcttcctcc
      721 agggcggcgc gttcctgttg caactgctgg tgttccgcgc gcagggcggc cagcgcggcg
      781 tccttcttct ggctggcgga gtaaacccag cccagcccgg ccaacagccc caccacacat
      841 aaccacggca atactttcat aacggtttat tttcttatcg gtcagaaggg caccttgtcg
      901 ttcccggccg aatgttgcgc cgccgccatg cgggcgtgcc atttccgcag gcggggatgc
      961 gccgggggca gccggtaatg ccccgagtag aggaagttgc ccaggatgcc gaagaggtcg
     1021 aaatccacaa accgcggctc gtgcgccagc aggaacgggc ggttccacag catcttctcg
     1081 aaaggctcca gccgttgcgt gaattgcgcc agcagctcct ttcgctgccg gcgccattgt
     1141 tccaggcagc cgcggccgaa cttgcgctcc ttgtggcgga caaannnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnntcgctg caggggatgt tggtaatttt aaagcgggcg
     1321 ccggagaact cgaggatgcg gcgctgaata atacagtaag ggctccaggg aaactgaaac
     1381 agctcaatca tattgcaggc agtaattcgc cacgggctac cctaagcggg cgtttcccgg
     1441 gcgacaagtg aaattgcgcc gggcgggcgg cggcgcgcgg tttctccggg ctccggccct
     1501 tttccctttt caggccgcgc gcggtgttta tcatactcgc gcaatgtttt cgcgcattca
     1561 atcggttcat tttatcggca tcggcggtac ggcgatggcg tccgtcgccg cggcgctgcg
     1621 ggaaaaaggg gtcaaggtca ccgggtcgga ccagaacatt tatccgccga tgtccacgtt
     1681 tctggccgag cggcagatcg aggtcaatcc cggctatgcc gagcccaacc tggcgcaccg
     1741 gccggacctg gtggtcatcg gcaacgccat ctcccggggc aacccggaag ccgaatatgt
     1801 gctggaccac aagctgccct actgttcgct gccggaactg ctgaaatact acttcctgcg
     1861 cggcaagcgt tcggtggtcg tcgccggcac gcacggcaag accacggtca cggcgctgct
     1921 ggcctgggtt tttgaacaca acggcctgaa cccgagctac ctcatcggcg gcattcccaa
     1981 caatttcagc cagggcgcgc gctttaccga cagcgagtgg ttcatcctcg agggggacga
     2041 atacgacacc gcgtttttcg acaagcgcag caagtttgtg cattatctgc ccgaggtggc
     2101 cattatcaac aacctcgagt ttgaccacgc cgacatcttt gacaacctgg aggccatcca
     2161 gacggctttc aaatacttca tccgcctgat cccccgcaac ggcctgctgc tcggcaacgg
     2221 cgacgatccg cgcctggcgc cgctgctcga cgtgacgcat tgcccggtga aacggttcgg
     2281 gctgggcgag atcaatgccg tccaggcctt caacctccgc tatggcccga cggcgtccga
     2341 gtttgagatc cccagcttta agtttcgcac caatttcctg ggggaaatca acgtgcgcaa
     2401 cgcgctggcc gtggtggcct gcgccaagca ttgcggcctc aagaaccagc agattcaatc
     2461 ggccttcgac accttcaaag gcatcaagcg gcgcctggag gtgcggggct tggcgggcgg
     2521 cgtgatcgtg ctggacgatt ttggccatca ccccacggcc atccgcgcca ccctcagcgc
     2581 gctgcgccag aaatatccca cccagcgggt ctgggccatc ttcgagccgc gcaccaatac
     2641 gacccggcgc agggttttcc aggccgaact ggccggcgcc ttcgcggagg ccgacgccgt
     2701 ggttatcgcg caggtggacc gcctggaatt gctggccccc gaagagcggc tcgacccgga
     2761 gcgcctgatg cgcgacttgc gggacgcggg caaggaggcc gtctatctgc ccgacgcgga
     2821 cgccatcgcg gcccatgtgg cgcagcaggt ccagggcggc gaggtggtga ttgtgttcag
     2881 caacggcggt tttggcggcc ttcatggcaa gctgctggcg cggctggggc ggggctgagg
     2941 cgggcggggc gtttcccggc ggtgattccc tgcttgaaat cgggaaaaag ggttttattc
     3001 tgagaagcaa tgagttgagg gccagggcgg cgcaaggcgc tttggtcggg taattaacag
     3061 aagagcacgc gattcatgcg aaagaaccgc cgcacctggc agtcgtttga agaagtgcgc
     3121 aaggccgccg atcaaggcga tgccgccgcg cagtgctacc ttggcatttg ctaccagacg
     3181 ggccaggggg tgccgcagga ttatgcggag gcggtcaagt ggtttcgccg ggcagccgat
     3241 caaaatgacg atacggcgca gtgctacctg ggattctgct accaatccgg cctgggcgtg
     3301 ccgcaggaat acgggcaggc cgccaaatgg tttcgcgagg ccgccgagcg gggagacccg
     3361 gcggcgcagt tcaacctggg ggtgctgtac gagaccggcc agggcgtgcc gcagaattac
     3421 gccgaagccg tgaagtggta tcacgccgcc gccgagcagg gcgagccgca ggcgcagttt
     3481 aacctgggag tcttttacga gaacggccag gtggtgccgc agaactatga ggaagctgtg
     3541 aagtggtatc gcagcgccgc cgagcaggaa tgcgccccgg cgcaatgcaa ccttggcctg
     3601 tgctacgaaa ccggccgcgg cgtgcccaag agcgtgcgcg aggcggtcaa gtggttctgc
     3661 cgcgccgcgc gggcggggga caagacggcc cagcacaacc tgggcgtcta ttatgcctcc
     3721 ctggaagcgg cggaggccgc cgccagcaaa gacgaacccc cggacgcggc gccccgcggc
     3781 aaggcgggat aacgacaacc cgggtttccg tgaacaaagg tttccgcacc aacagccgcg
     3841 cccgcagcgc ggaaccggcg ctgatccggt tctggggggt gcgcggttcc attcccgccc
     3901 ccggcccggg caccgttcgc tacggcggca atacttcctg cgttgaaatc cgcgccgatg
     3961 gccagatcat cattctcgac gccggcacgg gtttgcgcct gctggggcac catttgctcg
     4021 cggaagccaa agagcagccg cttgagttga cgttgctgct gacgcatacc cattgggatc
     4081 atattcaagg gctgcccttc ttttcgccgg tctatcaacc gcaaaaccac ctgcgtgttc
     4141 ttggctatga aggggcgcgg cacggcctgg cagtggtgct ggccggccag atggccaccc
     4201 ccttcttccc ggtccggctc cgcgaggtgc ccgccaatgt gcgcatcagg gagctcaaac
     4261 agaggcgctt ccagattggg ccggtccgcg tgcaggcccg gccagccaac catcccggca
     4321 aatgcgtcgg ctaccgcctg ttcacctcct gcgggtccgt ggccttcttt ccggatcatg
     4381 aggtgccggt ggcgaatgac gccggcgcgc ccgggcgcat ccccgcccct gcggccaacc
     4441 gggcgatgat ccaattcctg catggcgcgg acgtgctgat tatggacacg caattcgatg
     4501 ccgaggaata ccggcagcac cgcggctggg ggcacggctg catggacgcg gtggtggaat
     4561 tggcgctcca ggcaaaggtg aaaacgctct ttctcttcca ccatgccccc gatcacgatg
     4621 acgccaaagt cgcccgaatg ctggcgcacg cccggcggat tgttgcccgg cgaaaaggtt
     4681 cgctccgggt caacgccgcc cgcgaaggaa tgacggtcaa gctccctccg gcagagcggg
     4741 ttggcggggg agcggacgcc gcaacgaacg gctaacccat tgatcagtat ggggaagcgc
     4801 gagcagcgcc cgggggcaaa ggcggcattg gggggggctg catctggacg ggggcatgcg
     4861 ttggggcagg ctgattttgg agcaggggat tgtttcccgg cagcggcggc aactcaccgc
     4921 gatcaatcgc cgctttgttc cgcatcatgt aggccgcctc acgaatcgcc gcttcttccg
     4981 gcgtggtcgg ttccgggggc cagtttcttt tcaccgcgcc tggatgagtt gttggggttc
     5041 tcgtgggagg agtcggcgcg gcgggcttgc caggcgccgg cgtcgcggcc accttcacgc
     5101 cgttgttggt aaaatcgagg ctcgtaatgg tgccgtggtt gttcaccttc gccgtgcggg
     5161 ccaccgtgtc aatttccaga acttcaatct cgccatcgcg ctgccgctcg gacagggtca
     5221 gcgggatttc cttggccggt tccggcggtt tggcgggcat ggacaccttc atcaacaccc
     5281 gcttggtgcc gcccatctcg taaatacccg tgagcagtat cttgggcggc ggcggcttat
     5341 tggcttccgg atcaggcggt ggcggggggg gcttcagtcc aaaaacattc cgatctacaa
     5401 tcgcctggta gggattcccc ccggaggttt cgttccctgc cgcccggatc gccggccagg
     5461 ataccagcgc cgcgagcacg cacagcaaca gtttcccact gttcgtcata aggcaaatat
     5521 agtgtttgaa gagagaaaca ccactggaat cttttggttg tggagcaaag cgcggcgagc
     5581 gatcaatcga aaatgggata tctggcgaac gctccatacc accgggttct tgcggcttct
     5641 tagccccttc caaaggttat tgaagcgatc tctgacacca attcacccac ccacaccatt
     5701 acaacagaaa gcgtcgggtc aatttacagt cgttaaacca agccgatagc gcggcaaaaa
     5761 cgcaagctaa ccttcggtta ataggccact taggtttttg ctgcgctggt tgtaaagcag
     5821 tttggacctg tagctcgtcg ggaatcttta cactttatgc cacaaattct gtccgccata
     5881 gacgatttaa tgtctgtaaa ttattgataa tatacatctt agaactttgg catggttttt
     5941 ggcattggcc atgctaagta aatttgaact ttggcgagct taaaaagcag cctgtgattc
     6001 ccaacccgaa tcatgtgacg acctttttac gggcgcagag ccgcggctct gtccccgcca
     6061 gaagcattga ctgcaaatga acaagacaac gcgccgcatc gtcaagagcg ataggacggg
     6121 gaagacaacg ccccgcgcca acggcgaagt catctgccgc ggtgaactct ggcgcgccac
     6181 ggattcggta gtgaatgccc tgggcatgct ttggtgcgcg aagccggcgg agaagttcgt
     6241 ggttccgcca acgcacgatt gcagcggcga tcttccggac tgagtcagaa ccgcccctcc
     6301 caactcatcc cccacaggcg cagccgccgc cggctgcgcc tgaattatat gggcgcggtg
     6361 ccggcaagcc gcgtttccac actatttccc gtgacatccg gcgggcgctg cggtctaatt
     6421 ctttgtgagc cgtgttgtga gcgagccgtt ggcagaacgt gagggtccgg atgcgccgca
     6481 agccgggctg gccccgtccg cattcgccga tctgtatacc cggcacagcc gcgccatcta
     6541 ctatttatgc ctgcggctgt tgaatgaccc tggcaaagcg gaagacgcga cccacgatgt
     6601 ctttctgaag gcgttttgca aacggaacca gttccgcggg gaatcttcgt ggcgcacctg
     6661 gctttaccgg atagcgatca accattgccg caacctgctg gcggcgtggc atgaacggca
     6721 catgctcagc aacgcggatg aggccctctg ggagaacacc gcggcgcgga ccgactcgcc
     6781 gttgcgcgtg gtggaaatcc gggagctggg cgagcgcatc cagcaggcgc ttgtggggct
     6841 gcccggggaa taccggctgc ttttattgct ggtagccgat gaagaattga gttatgaaca
     6901 ggtgggcgcg ctgacggggc agtccgccga tgcggttcgc ggcaagctgc accgcgcgcg
     6961 caaggctttt atcgtccggt ttcgccaaac ggaatgagat ggaaagatga aacgtcaaac
     7021 caaactgacc gcgcaggaac aggaacaact cgacgcccgg caacagcaag cagcggccgc
     7081 cgccccgcgg gagtttgcca gcgtggagga aatgcttcgc catgatgcgc agtgtacgcc
     7141 cgtgccgccg gccatcgcgc gccggctgga aaagtccgtg cgcgatctgc ctccgccgcc
     7201 cgcccgcgcc tggtggcggc gtttttttgg cagttgatcc cgtgccctcg cgtccgtcgt
     7261 tatgagcttt tccgacgaag ttgagatcct catccgcgcc cgttatccca tcctttatat
     7321 catcaccagc gaggagctgc gggtggaaaa gattctgcgg caaattgccg ccgggcggca
     7381 gaagaaggtt ttcgagtgga cctgcagctc cggcgtagtg gcggccggca cgccgtttca
     7441 atcccaaaaa ggccgcaacc ccgccacccg ggaaccgctg gcagcgctgg atttggtggt
     7501 ggaacaactc gagccggccc tgttcctgtt caaggacttc catccgtttc tggcgcgggg
     7561 caatttcgcc atcatccgcc gcctcaagga tatcgcgctc cacctcaaaa acagccgcaa
     7621 gaccatcgtg ctgatctcgc cggtgctgga aatcccgccc gagctggaca aggagatcac
     7681 cgtcttgaac tttccgcaac cgggcaaaga cgagttgggc gagctgctgg acaaagccgc
     7741 cgccgaactg cgggagtccg gggaggtgcc ggttgagctg gacgaaccgg gccgggaccg
     7801 gctgctgcag gcggcactgg gcctcacgct gggggaggcg gagaatgttt ttgccaaaat
     7861 catcgtcaag aaccagcggc tcagcgagga cgccgttggc gaggtgatcg ccgagaaaca
     7921 gcaaatcatc cgcaagagcg gcctgctgga atattacgag gccgaggagg acttcgacca
     7981 cgtgggcggc ctggacgtgt taaaggactg gataaacaag cgtgctgcgg ccttcaccga
     8041 cgaggcgcgg gcgtttgggc tgccggcccc caggggggtg ctgctgctgg gcgcccaggg
     8101 ttgcggcaag agcctttgcg ccaaagctgt ctcgcggcgc tggcaattgc cgctgctgcg
     8161 ctttgatatg ggccgcatgt tcagcagcct ggtcggctcg tcggaggaaa atatccgccg
     8221 ggccattgcg gtggctgaat ctattgcgcc ggtcgtgctt tgggtggatg agattgacaa
     8281 ggcctttagc ggttcccaaa gctccggctt caccgacggc ggcaccacgg cccgggtctt
     8341 tggcaccttc ctgacctggc tttcggaaaa gcgcgccccg gtgttcgtcg tggccaccgc
     8401 caacgacgtt tcccagctcc cgccggagct gctgcgcaag gggcggctgg acgaaatctt
     8461 ctacgtggac cttccccggc ccccggaacg ggccgacatc ttccgcatcc acctggcgaa
     8521 gcgccaccgc catcccggca acttcgacct gcccgcgctg gtggcggcca gcccggagtt
     8581 cagcggtgcc gagattgaag cggcaatcat cagcgccctc tacgacgcct tctacgcgca
     8641 gcaggaattg accaccgcgc acgtgctcga agcgctgcat caaaccgtgc cgctggccag
     8701 aaccctggcc gagaaaatct cggaacaacg cgcctgggcc gcgggccgcg cccgcaacgc
     8761 cagcatcgcc agctcagttc gggatctggc ggaagcgcgt gtcccgtagg cgctccgccg
     8821 gcaaaggcgc gtgggatgat ggctgccgcg gctttgccgg cggctccgcg ccccgcagtc
     8881 ggaggcccga aaacggcatt attcgacgat tcgggggacg atgaacagac cgttggcgcg
     8941 cgccggggcg ttgcgcagag cctcttcgtt gctgagggaa gggcgaattt catccggccg
     9001 ggcgacgtta accagcggga aggcgtgcgc ggtcggatcc acgccgctga catccacctc
     9061 tttcagtttc tcgatgtagc cgaggatatt gcccatttgc ccgcccagcg tctgttcctc
     9121 ttcgggcgag agggagagcc gcgccaggtg ggcgatatgc ttcacgtcaa attcagccgt
     9181 attcatggga gagttgccgg gttgcgccgc cgccggaggg aaggcgccgc catcatcatt
     9241 cctcgtcaaa tttgcgattg atgagggttt ccagttcgag aagggcctgg gaggcatcct
     9301 ggccctgggc at
//
LOCUS       DAOPQG010000131         9201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_8478_length_9201_cov_16.041986, whole genome shotgun sequence.
ACCESSION   DAOPQG010000131 DAOPQG010000000
VERSION     DAOPQG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8478_length_9201_cov_16.041986"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKI07_12245"
     CDS             complement(<1..260)
                     /locus_tag="PKI07_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51854.1"
                     /translation="MLTAIDHWLRTGKPSVVKESPRLDDALKEYTTWMDATEELRLQT
                     KLNLRRRVTNFVKGVGNKRVIDVVPEHVEQYLASRNVSPITK"
     gene            complement(369..1436)
                     /locus_tag="PKI07_12250"
     CDS             complement(369..1436)
                     /locus_tag="PKI07_12250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC51855.1"
                     /translation="MTPPPPPPAPPPPLLSVILLNYNGARWLERCLQSLHSQTLFDQL
                     QVILADNASSDNSEPLARPIVESWPNGMFLPHGQNLGYCEGNNRAAEHARGQYLLFLN
                     NDTWLEPRCLEVLLAETQRAGAAAAGPLVLDYEDDRFQSLGAFGFDLFGLASTRVPAA
                     TTRPVFMPEGCAYLIRRDLFHQLGRFDPQFFMFADEYDLSWRVWISGHQAVAVPAARL
                     HHRGAAQVNPAGGGATVEFRTSDTKRFYANRNHLLTLLKNARGLLLLLVPLQLALLAL
                     EALVSLVLIRRWTFVRRAYGDAVIDCWRLRKHIRAERRRIRQFRRRGDGWMLRFLRVR
                     CNRWDELQRLRRFGAPKVTPR"
     gene            complement(1440..2504)
                     /locus_tag="PKI07_12255"
     CDS             complement(1440..2504)
                     /locus_tag="PKI07_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC51856.1"
                     /translation="MNILVISLAGIGDTLFATPLIHELRANFPAARLDALVLWPGARD
                     LLEGNPHLNTVYQKNLLRAGKAEALRFLWHLRRQGYDLSLNTHPQSRVHYRAVARVIN
                     ARVRISHQYDNACALDRLLVNRTLPQDYTRHAIENNLALLEAAGARRTLPRHEYEVFL
                     TAAETQWAGDYLARHQWAGRTLLGVHVGSGGTKNLPLRRWPLDHYLALFQELRQSHPE
                     LGILLLGGPEEAQPHQRLAAQTCHEQVRVAQTGSLRQAAALLQRCAAFLSVDTALMHL
                     AAAMKVPRLVVIETPTWNKPIEPYGHPFTLIPNPAVAGRNLDYYRYDGRGIRGSPAEL
                     LRCMSAVSVPAVAQALRRLL"
     gene            complement(2517..3002)
                     /locus_tag="PKI07_12260"
     CDS             complement(2517..3002)
                     /locus_tag="PKI07_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOC51857.1"
                     /translation="MNFRGKIIAWDELPAWRAARRARGQRLVVTNGCFDLLHLGHVTY
                     LETARKQGDALLVGVNGDAAVRQLKGPERPVTPADDRAAVLAALESVDGVCIFAETTA
                     TRFLAAAQPDIYVKGGDYTLETLNQEERRAVERAGGRIVIIPFVPDRSTTATLRKIMR
                     L"
     gene            complement(2999..3925)
                     /locus_tag="PKI07_12265"
     CDS             complement(2999..3925)
                     /locus_tag="PKI07_12265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC51858.1"
                     /translation="MPGTAAQAAKHIPTARRRGRILLAESAELAQTGASVANIYYDPG
                     AARAAKVQALFSRIAPRYDLINDVQSLGLHRLWKRRVIQLARPRPGEHALDLCCGTGD
                     LALALARRGAQVIGLDFNERMLAAAKRRAAAFRPRAGTPPPSPAAPSEAPPAALRFVR
                     GDAQCLPFPDASFDLVTIGYGLRNLASWEAGLGEMQRVARPGGRLVVLDFGRPDNALW
                     RRLYFAYLRRLVPCLGWLFCGSASAYAYILESLQHYPAQHGVAAAMRGHGLANVRVLS
                     LLGGVMAINYGEKPGPSLSPCQGRNGLARMPG"
     gene            3940..4389
                     /locus_tag="PKI07_12270"
     CDS             3940..4389
                     /locus_tag="PKI07_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC51859.1"
                     /translation="MRHRKILVVDDSPVILKTLSMKLTVHGYDVVTAEDGSAAIGAVR
                     RHKPDLILLDLSFPPDVAHGGGVAWDGYLIMNWLRRLEEGRNIPILVITGGDPDQCKE
                     RALAAGATGFFHKPINSDELLAEIEQTLAKQANAPPPAAAAPAAPAP"
     gene            complement(4418..5230)
                     /locus_tag="PKI07_12275"
     CDS             complement(4418..5230)
                     /locus_tag="PKI07_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC51860.1"
                     /translation="MKFAICNEIFQGWNMEDILAYAARIGYAGVEIAPYTLAPSVTDI
                     SAGERRRLRECAARHGVAIVGIHWVLVKPEGLYLNHPDRARRERTAQYFCDLVDFCAD
                     LGGQVMVVGSPKQRNILPGVSPEQAREWAAATFRPAVARAEQRAVTICFEPLAPAETN
                     FINTAAEALQFIQLVPSPRFRIILDVKAMCSEAKPIPQIIRESWPHFAHVHANDRNLK
                     GPGFGEVDFRPIAAALREVGYQGFVSVEVFQFEDGAETIAVRSLEYLRSAFA"
     gene            5390..6536
                     /gene="prfB"
                     /locus_tag="PKI07_12280"
     CDS             join(5390..5458,5460..6536)
                     /gene="prfB"
                     /locus_tag="PKI07_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811797.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOC51861.1"
                     /translation="MFETIKAELVTATDKLAHLRRFLDVPNAQRRLGEINALMAAESF
                     WNNREQAQKLIDEANSIRSRIEPLLKAERQVEDFEVMVELGEAEPEAAQPQLRQELER
                     DLTGFFKDLEALELRAFLNGPHDQRNCIVSINAGAGGTESCDWAGMLLRMYQRWAEMR
                     GWRVEVEDVLPGEAAGIKSATLIISGEKAYGYTKAERGVHRLVRISPFDANKRRHTSF
                     ASVDVIAEIEENDADLTIPPNEFQIDTFRSGGKGGQNVNKVETAVRITHLPTGLVVAS
                     QSQRSQHQNRAAAMKLLLSRIYAQRLDAQKAEMERFYGEKGSISWGNQIRSYVFQPYR
                     MVKDLRTGVETSNVQAVMDGDLDPFITAWLRAGCPNKRMQGIRDEED"
     gene            6623..7432
                     /gene="trpC"
                     /locus_tag="PKI07_12285"
     CDS             6623..7432
                     /gene="trpC"
                     /locus_tag="PKI07_12285"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOC51862.1"
                     /translation="MNILDTIVAQKQREVARLPQRPVSAAAIAAAQAAGGPRRDFLAA
                     LRNPPPPGPALIAEVKKASPSAGVICPNFDPVLIARAYEAAGASCLSVLTDERFFQGS
                     LSYLKEIRAAVKLPLLRKDFLIDERQVLEALEWGADAVLLIAAILDDAQLRHLHALAR
                     EAGLAALVEVHDEAELDRARAAGAELIGINNRDLKTFQVDLATTERLAARLRAAPPAA
                     GPPPLLVAESGIRTRADVVRLTRCGARAILVGETLVRHPDLEARVRTLLGG"
     gene            7517..8914
                     /locus_tag="PKI07_12290"
     CDS             7517..8914
                     /locus_tag="PKI07_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51863.1"
                     /translation="MQTPVSAPFSPARFNISRRRFLQSASACAALAALPALGADALDL
                     VQRKPRRVGLIGAGWYGKSDLWRLVQVAPVEIVSICDPDQRLLAEAVEMARQRQKSGK
                     KPRAYGDYRQMLAAHDLDIVLVGSPDHWHALHTIAALEAGADVYVQKPISADVLEGEA
                     MLAAARRHRRVVQVGTQRKSTPHLIEAKRQIVDAGLLGRIGHVDMCCYFPMRANGNPP
                     VEPVPDFFDYEMWTGPAPLRPYDGLPHVRWWRTFMEYGNGIVGDMCVHMLDTVRWMLG
                     LGWPKRISSAGGIYVQKEGKSNISDTQTATFEYDQFHCVWQHRTWGAPTDPDYPWALF
                     IHGEKGVLKASTLRADFVPVDKNSPPRHFECVYEKEQYPEDVTEKGIELNAAPATRRH
                     LLDFLAAIEQRSRPIADIEEGHISTASCILANLAMQLGRALVYDPRKREVVGDRAATK
                     LLRRPYRQPWKHPLV"
     gene            complement(8989..>9201)
                     /locus_tag="PKI07_12295"
     CDS             complement(8989..>9201)
                     /locus_tag="PKI07_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51864.1"
                     /translation="PVAYHDPLLFAELLGHYRLNGGGACQSECLSWVAGVEAFAKYCE
                     ISSYAQTTPPEASREAWFLPGKRHLP"
BASE COUNT         1617 a         3122 c         3063 g         1399 t
ORIGIN      
        1 tccttggtaa tcggcgacac attccggctg gccaggtatt gctcgacatg ctcgggcacc
       61 acatcaatca cccgcttgtt gcccaccccc ttgacgaagt tggtcacgcg ccgccgcagg
      121 ttcaacttcg tctgcaggcg caactcctcc gtggcatcca tccaggtggt gtattccttc
      181 aacgcatcat ccagccgtgg cgactccttg accaccgacg gtttcccagt ccgcagccaa
      241 tggtcaatgg cggtgagcaa gtcctggtcg cgctccagcc gtttgaaccc ggcctcggca
      301 atggcgacct gctcatccgt gagccgggta gtccgcagtg ccgccgggga tttttgcccg
      361 gctcccgttc agcggggggt gactttgggc gcgccgaagc ggcgcaggcg ttggagctcg
      421 tcccagcggt tgcagcgcac gcggaggaaa cgcagcatcc acccgtcgcc ccggcggcgg
      481 aactggcgga tgcggcggcg ctcggcccgg atatgcttcc gcagccgcca gcaatcaatc
      541 acggcatccc cgtaggcgcg ccggacgaag gtccagcggc ggatgaggac caggctgacg
      601 agggcttcga gggcgagcag ggcgagttgg agcggcacca gcagcaagag caggccgcgg
      661 gcgttcttga ggagcgtcag cagatggttg cggttggcgt agaagcgctt ggtgtcgctg
      721 gtgcggaact cgacggtcgc gccgcccccg gcggggttga cctgcgccgc gccgcgatgg
      781 tgcagccgcg ccgccggcac cgccaccgcc tggtgcccgg aaatccagac ccgccaggac
      841 agatcgtatt catccgcgaa catgaagaac tgcggatcga agcggcccaa ctggtgaaag
      901 agatcgcgcc ggatgaggta ggcgcacccc tccggcatga acaccgggcg cgtggttgcg
      961 gccgggacgc gcgtgctggc caggccaaag aggtcaaagc cgaaggcccc gagcgactgg
     1021 aagcggtcgt cttcgtaatc gagaaccagc ggcccggcgg cggcggcgcc ggcgcgctgc
     1081 gtttcggcga gcagcacctc caggcagcgg ggttcaagcc aggtgtcgtt gttgaggaaa
     1141 agcaggtatt ggccgcgcgc gtgttcggcg gcgcggttgt tgccctcgca atagccgagg
     1201 ttttggccgt gcgggaggaa catgccgttg ggccagcttt cgacgatggg ccgggccagc
     1261 ggctccgagt tgtcgctgga agcattgtcg gcgagaatca cctggagctg gtcgaagagg
     1321 gtctgggagt ggagcgactg gaggcagcgt tcgagccagc gggcgccgtt gtagttgagg
     1381 aggatgaccg aaaggagcgg cggcggcggc gcggggggtg gcgggggcgg ggtcatcggt
     1441 caaagcaggc ggcgcagggc ctgggcaacg gcgggaacgg agacggcgga catgcagcgg
     1501 agcagctcgg cgggcgaacc gcggatgccg cgtccgtcgt agcgatagta gtcaaggttg
     1561 cgcccggcga cggcgggatt ggggatgagc gtgaaggggt ggccgtaagg ctcgatgggc
     1621 ttattccagg tgggcgtttc gatgaccaca agccggggca ctttcatggc ggcggccagg
     1681 tgcatgaggg ccgtgtccac gctgagaaaa gccgcgcagc gctggagcag cgcggcggcc
     1741 tggcgcaggc tgccggtctg ggcgacccgc acctgctcat ggcatgtctg cgccgcgagg
     1801 cgctgatgag gctgggcctc ctccggcccg cccaggagca ggattcccag ctcgggatgc
     1861 gactggcgca gttcctggaa gagggcgagg taatggtcga gcggccagcg ccgcaggggg
     1921 aggtttttgg tgccgccgga gccgacatgg acgccgagca gcgtccgccc ggcccactgg
     1981 tggcgggcga ggtagtcccc ggcccactgg gtttcggcgg cggtgaggaa gacttcgtat
     2041 tcatggcgcg gcagggtgcg gcgggcgccg gcggcttcca gcagcgcgag attgttctcg
     2101 atggcgtgcc gggtgtagtc ctgcggcagc gtgcgattga ccagcagccg gtccagggcg
     2161 caggcgttgt cgtattggtg actgatccgg acgcgggcat taatgacgcg cgccacggcg
     2221 cggtaatgca cccggctttg ggggtgggtg ttgagggaga ggtcgtagcc ctggcggcgc
     2281 aagtgccaca ggaaccgcag ggcctcggcc ttgcccgccc ggagcaggtt cttctgataa
     2341 acggtgttga gatgggggtt gccctccagc aagtcccgcg ccccgggcca cagcaccagg
     2401 gcgtccaggc gggcggcggg gaaattggcg cgcagctcgt gaatcagcgg cgtggcgaac
     2461 agcgtatcgc cgatgcccgc cagtgagatg accagaatgt tcattcccgg cccgggctac
     2521 aggcgcatga ttttccggag ggtcgccgtc gtggatcggt ccggcacgaa ggggatgatt
     2581 acaatccggc cgccggcgcg ctcgacggcg cggcgctcct cctggttgag ggtttccaag
     2641 gtgtaatcgc cgcccttgac gtagatgtcc ggctgggcgg cggccaggaa gcgcgtggcg
     2701 gtggtttccg caaagatgca gacgccgtcc acgctctcca gcgcggccag cacggcggcg
     2761 cgatcgtcgg cgggcgtcac cgggcgttcc ggccctttca actggcgcac ggccgcgtcg
     2821 ccattgacgc cgaccaggag ggcgtcgccc tgcttgcggg cggtctccag gtacgtgacg
     2881 tggccgaggt ggagcaggtc gaagcagccg ttggtgacca ccaggcgctg gccccgggcg
     2941 cgccgggcgg cccgccacgc cggcagttca tcccaggcaa tgatcttccc gcgaaagttc
     3001 accccggcat tctggccaga ccatttcggc cttggcaagg cgagagagag gggccgggtt
     3061 tctcgccata attaatggcc atcacgccgc cgagcaggga aagcacccgc acgttggcca
     3121 ggccatggcc gcgcatggcc gccgccacgc cgtgctgggc cgggtaatgc tggagggatt
     3181 ccagaatgta ggcgtaggcg ctggcgctgc cgcaaaagag ccaccccagg cagggcacca
     3241 accgccggag atacgcgaag tagaggcgcc gccagagcgc gttgtccggc cggccaaaat
     3301 ccagcaccac cagccgcccg ccagggcggg cgacccgctg catttcgccc aggccggctt
     3361 cccagctcgc caggttgcgc aagccgtagc caatcgtcac gaggtcaaag gaggcatccg
     3421 gaaaaggcag gcactgcgca tcgccgcgga caaagcgcaa agccgcgggc ggagcctccg
     3481 agggggcggc gggagaagga ggcggcgtcc ccgcgcgcgg gcggaaggcg gcggcgcgcc
     3541 gcttggcggc ggccagcatg cgctcgttga aatccaggcc gatgacctgc gcgccgcgcc
     3601 gggccagcgc cagcgccagg tcgccggtgc cgcagcataa atccagcgcg tgctccccgg
     3661 gccggggccg ggccagttgg atcacgcgcc gcttccaaag ccggtggagg cccaggctct
     3721 gcacatcgtt gatcaagtcg tagcgcggcg cgatgcggga gaaaagcgcc tgcaccttcg
     3781 ccgcgcgcgc ggcgccggga tcgtagtaga tattggccac ggacgcgcca gtttgcgcca
     3841 actccgcgga ttcggccaac agaatccgcc cccgccgccg ggcggttgga atatgttttg
     3901 ctgcctgagc ggcagtgccc ggcacgcgct aaattattta tgagacaccg aaagatactg
     3961 gttgtggatg acagccccgt cattttgaag accctgtcca tgaaattgac ggtgcatggc
     4021 tacgatgtag tcacggcgga agacggcagc gcggcgatcg gcgcggtgcg gcgccacaaa
     4081 cccgacctca tcctgctcga tttgagtttc ccgcccgacg tggcccacgg cgggggcgtg
     4141 gcctgggacg gctatttgat catgaactgg ctgcggcggc tcgaagaagg ccggaacatt
     4201 cccatcctgg tcattaccgg cggcgacccg gaccaatgca aggaacgggc actggcggcc
     4261 ggcgccaccg gcttcttcca caagcccatc aatagcgacg agctgctggc cgagatcgag
     4321 cagacgctgg ccaagcaggc aaacgccccg ccgcccgccg ccgccgcgcc ggcggccccg
     4381 gccccataat cggcgggccg ccccgccccg gcccctttca ggcaaacgcg ctccggagat
     4441 actccaggct ccggacggcg atggtctccg cgccgtcttc gaactggaag acttccaccg
     4501 agacgaagcc ctggtagccc acctcccgca gcgcggcggc gatgggccgg aaatccacct
     4561 ccccaaagcc ggggcctttg aggttgcggt cattggcatg cacgtgggca aagtgcggcc
     4621 aggactcgcg gatgatttgc gggatcggtt tggcctccga acacatcgcc ttcacatcga
     4681 ggatgatccg gaaccggggc gaggggacca actggatgaa ttgaagggcc tcggcggcgg
     4741 tgttgatgaa gttggtctcc gccggcgcca gcggctcaaa gcaaatggtg accgcccgct
     4801 gctccgcccg ggcgaccgcc gggcggaacg tcgccgccgc ccattcccgc gcctgttccg
     4861 gggagacgcc cggcaggata ttccgctgtt tgggcgagcc gacaaccatc acttggccgc
     4921 cgaggtcggc gcagaaatcc accaggtcgc aaaagtattg ggccgtgcgc tcacgccgcg
     4981 cgcggtcggg atgattgaga tagagcccct cgggcttgac cagcacccaa tggatgccga
     5041 cgatcgccac cccgtgccgc gcggcgcatt cccggagccg gcgccgctcg cccgccgaaa
     5101 tatccgtcac cgacggggcg agcgtgtagg gggcaatttc caccccggca tacccgatcc
     5161 gggcggcata ggccaggatg tcttccatat tccacccttg aaatatctcg ttgcagatgg
     5221 cgaatttcat ccccgccagc ttcgggcttc cggcgaggcg attcaagacg gaaagacccc
     5281 ggccccgccg cccaacacac gcaaccccct ctggccgccg cccgcgccgc cgctcctccc
     5341 gttcccggtt ccacttgcat atcccgccgc cgcgccctac cctgcagata tgttcgagac
     5401 aatcaaagcc gagttggtca ccgccacaga taagctggct catctgcgga ggtttctttg
     5461 acgtgcccaa cgcgcagcgc cgcctggggg aaatcaacgc cctgatggcg gccgagagtt
     5521 tctggaacaa ccgcgaacag gcccagaagc tcattgacga ggccaattcg atccgcagcc
     5581 gcatcgagcc gctgctcaaa gcggaacggc aggtcgagga cttcgaggtc atggtggaac
     5641 tgggcgaagc cgagcccgaa gccgcccagc cccagctccg gcaggaactg gagcgggacc
     5701 tcaccgggtt tttcaaggac ctcgaggcgc tcgaactgcg ggccttcctg aacggccccc
     5761 acgaccagcg caactgcatc gtcagcatca acgccggggc gggcggcacg gaatcctgcg
     5821 attgggcggg catgctgctg cgcatgtacc agcgctgggc cgagatgcgc ggctggcggg
     5881 tcgaagtcga ggacgtgctc ccgggcgaag cggcgggcat caagagcgcc acgctcatca
     5941 tcagcgggga aaaagcctac ggctacacca aggccgagcg cggcgtccac cgcctggtgc
     6001 gcatctcgcc cttcgacgcc aataaacgcc gccacaccag tttcgccagc gtggatgtga
     6061 ttgccgaaat cgaggagaac gacgccgacc tcaccatccc gcccaacgag ttccagattg
     6121 acaccttccg ctccggcggc aaaggcggcc agaatgtcaa caaggtggaa accgccgtcc
     6181 gcatcacgca cctgcccacc gggctggtgg tggcgtcgca aagccagcgc tcgcagcatc
     6241 aaaaccgcgc cgccgccatg aaactgctgc tctcgcgcat ctacgcccag cgcctggacg
     6301 cgcaaaaggc cgagatggag cgcttctacg gcgaaaaagg cagcatctcc tggggcaacc
     6361 agatccggag ctacgtcttc cagccctacc gcatggtcaa agacctgcgc acgggcgtcg
     6421 agaccagcaa cgtccaggcc gtcatggacg gagacctcga ccccttcatc accgcctggc
     6481 tgcgcgccgg ctgccccaat aaacggatgc agggaatccg ggatgaagaa gactgaccgt
     6541 ctcccgcttc ccgcgcccca acccgccccc gctttgaacc ggcggagccg gctggcccgg
     6601 cgcaatacgc cctttcgcta acatgaatat cctggacacc atcgtcgctc aaaaacagcg
     6661 ggaagtcgcc cgcctgccgc agcgcccggt ttcggccgcc gccatcgccg ccgcgcaggc
     6721 ggcgggcggc ccgcggcggg acttcctggc cgcgctgcga aacccgccgc cgcccggccc
     6781 cgcgctgatc gccgaggtca agaaggcttc cccctccgcc ggcgtcatct gcccgaactt
     6841 cgatcccgtc ctgattgccc gggcctacga agcggcggga gcgagctgcc tttccgtcct
     6901 gacggatgag cgcttctttc aggggtcgct gtcctatctc aaggagattc gcgcggcggt
     6961 gaaactgccg ctgttgcgca aggattttct cattgacgag cgacaggtgc tggaggccct
     7021 cgagtggggc gccgacgccg tcctgctgat tgccgcgatc ctggacgacg cgcagttgcg
     7081 gcatttgcac gcgttggccc gcgaagcggg cctggccgcc ctggtcgaag tgcatgacga
     7141 agccgagctg gaccgcgccc gggccgccgg cgccgagctg atcggcatca acaaccgcga
     7201 cctgaaaacc tttcaggtgg acctggcaac caccgagcgc ctggccgcgc gcttgcgcgc
     7261 cgcgccgccc gccgccggcc ctccgccctt gctggtcgcg gaaagcggca tccgcacccg
     7321 cgccgatgtc gtgcgcctga cccggtgcgg cgcccgggcc atcctcgtgg gcgaaaccct
     7381 ggtcaggcat ccggacctcg aagcccgcgt ccgcaccttg ctgggcggct gaccccgcgc
     7441 ccggctccgt gcccggcccg cggattgccg cacgccgggg ttgcttccgc cgcccggccc
     7501 gcctaatact gggcccatgc aaactccagt gtctgcgccg ttctctcccg cccggttcaa
     7561 catcagccgc cgccggttcc tccagtccgc ttccgcctgc gccgccctgg cggccctgcc
     7621 ggcgctcggc gcggacgcgc tggacctggt gcagcgcaag ccccggcggg tcgggctcat
     7681 cggcgcgggc tggtatggca aaagcgatct ctggcgcctg gtccaggtgg ccccggtcga
     7741 gatcgtctcc atctgcgacc ccgaccagcg gttgctcgcc gaggcggtcg aaatggcccg
     7801 gcaacggcag aaatcgggaa agaagccccg cgcctacggc gattaccgcc aaatgctcgc
     7861 cgcgcatgac ctggacatcg tcctggtcgg cagcccggat cactggcacg ccctgcatac
     7921 catcgccgcc ctcgaggccg gggcggatgt gtacgtgcaa aagcccatca gcgcggacgt
     7981 gctggaaggc gaagccatgc tggccgccgc ccgccgccac cgccgcgtcg tgcaggtcgg
     8041 cacccagcgc aaaagcacgc cccatctgat cgaagccaaa cggcaaatcg tggacgccgg
     8101 cttgctgggc cggatcggcc acgtggacat gtgctgctac ttccccatgc gggccaacgg
     8161 caacccgccc gttgagccgg tgcccgactt cttcgactac gaaatgtgga ccggccccgc
     8221 cccgctgcgg ccctacgacg gcctcccgca cgtccgctgg tggcgcacct tcatggaata
     8281 cggcaacggc atcgtcggcg atatgtgcgt ccacatgctc gatacggtgc gctggatgct
     8341 ggggctgggc tggcccaaac gaatctcctc cgccggcggc atctacgttc aaaaagaagg
     8401 caagtccaac atctccgata cccagaccgc aaccttcgaa tacgaccagt tccattgcgt
     8461 ctggcaacat cgaacctggg gcgcccccac cgacccggac tacccctggg cgctgttcat
     8521 ccacggtgaa aaaggcgtcc tcaaagccag caccctgcgc gccgacttcg tcccggtgga
     8581 caagaactcc ccgccccggc atttcgagtg tgtttacgaa aaagagcaat accccgagga
     8641 cgtgaccgaa aaagggattg aactcaacgc cgcgcccgcc acccgccgcc acctgcttga
     8701 tttcctggcc gccatcgaac agcggagccg gcccatcgcc gatatcgagg agggccacat
     8761 ctccaccgcc agttgcatcc tcgccaacct ggcaatgcaa ctcgggcgcg cactggtcta
     8821 tgacccccgg aaacgcgagg tcgtcgggga tcgcgccgcc accaaactcc tccgccgccc
     8881 ctaccgccaa ccctggaaac atcccctggt ttagccccgg cgccgcatcc aaatcggcat
     8941 aaaagcccat cgaaaacgcg aaaaaccggg gaatcggccg ccgctccatt acggcaaatg
     9001 gcgtttcccg ggcaaaaacc atgcctctcg cgacgcttcc ggcggggtgg tttgcgcata
     9061 gctgctgatt tcacagtatt tagcaaacgc ttccaccccg gctacccagc ttaaacattc
     9121 gctctggcac gccccacccc catttagccg gtagtgtcct aaaagttctg caaaaagcag
     9181 cgggtcatgg taagccactg g
//
LOCUS       DAOPQG010000132         8987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_8723_length_8987_cov_12.369682, whole genome shotgun sequence.
ACCESSION   DAOPQG010000132 DAOPQG010000000
VERSION     DAOPQG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8723_length_8987_cov_12.369682"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..2987
                     /locus_tag="PKI07_12300"
     CDS             363..2987
                     /locus_tag="PKI07_12300"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HOC51865.1"
                     /translation="MPNYRCYRRDGSEILGWGVNQCDQNGWWNFWTGNFNGNSDRFWR
                     FRIRGEDSAKCGTANTIVACRARFYGAKWYYINDWVCLGGYSSSSVSDTHGFDEANAY
                     LYPAIDTSHGNVIAPALFNGKVPGRVKTGDCNHANSLNFKGNAASYGGGDNMDSYGFA
                     WAYAPEGAGPKFLIGSDDGNRVWVNGTRINDNNAYRGLTRDADETGGIGFQKGWNRIL
                     FKVHNGGGGFEGTISLRNGGTRDWNEPSVTVFDMGNGVYSFGAGFEQDWWYPCIGVAN
                     FYGASNPQPNSNHYGNNTTVAASGSAQGCGPVPFWKVMQFQWGYGIPVSNYADVSASG
                     TSWSHTQTGVTGHRRFHFFAVSRSGRTSFQNSGVSGGWNWVGGGSGNYMDVYVDNVAP
                     VNPSFTTASAVSTTQINLAWAIPLDQGVGIVAGATEAADETSDSSANYYRRGDVGVQV
                     YRDAVAISAWGVGTSKNDTGLTPNNPYTYTIEARDNNSQSRGAWANTTGRQGATVVWT
                     LSMPPVAGSVVPDNLTPCAGSPVTWSATEGFGAGSVAKYKYAWNQSPTYAWTGSEPDW
                     SVDTLQTIPAVAGTWYLHVKGYNGAGVENGTHAYAITTLAAPTTTGIAGSSAVAIGQV
                     GEIYTVTPPPASGSSFVWSVPGGAVITAGGSGPNNSQIVVTFGSSSGDVTVRETTAGG
                     CEGPLVMKGVTVGPNRAPVAGAVKTISTTKNTPVTIYKVKLLAGATDADGDPLSISAA
                     GPVSAEGGQVEERANDVKYTPQVDFWGPDSFTYTISDGQGGTAVGTVEVMVSYGGGLS
                     PNLVYWPTHDGTMFRVTFAGIPGKEYGVEWSEAPSGMPWTWLKNATAGPNGLFEVTDV
                     VSEVPARYYRIVYPPPQP"
     gene            3067..3807
                     /locus_tag="PKI07_12305"
     CDS             3067..3807
                     /locus_tag="PKI07_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOC51866.1"
                     /translation="MKLRFLAIGVVWASMTMLVQGEWATFTYSSGFQNGGVIPDNSLV
                     GWADTRQVSGLVGEILDVNVGLQLSGGWNGDLYSWLVHSTGFAVLLNRVGGTGVNPPG
                     YGDAGMSMMLDDQGEKGDIHWYGGGFIPTTTAWPTVPDGAPTPSDNWKPDGRYVDPID
                     GDVGVAPRTALLSSFNGLNPNGDWTLFIADVAAGETSTLTSWTLNIQTAAVPEPASLV
                     QGAVVVLLMGGVMGGYRLRGQKRRFHSH"
     gene            4177..5718
                     /gene="xrtU"
                     /locus_tag="PKI07_12310"
     CDS             4177..5718
                     /gene="xrtU"
                     /locus_tag="PKI07_12310"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase U"
                     /protein_id="HOC51867.1"
                     /translation="MLGYAPLLGLFFLNLWGRPHYQFFPWALLGAGFLAWIRGKELSR
                     PFVPGDRKVTMFLQGVSFLLLGLATVSWSPWLACLAALTGLVAAIWGRGGSKLLNAML
                     PALILLLIIIPPPLLADNLLIQQMRVLAVRGSSSVLDSLGVIHALSGNVIEIPGQRLL
                     VDEACSGINSVLVLLACALFYGLWRRRSPLHILLSLVNALGIVLLGNLARITLGAWVK
                     FRYGIDILTGTVHQVAGLLLFPTYIGIILSMDQLLAYLLNPIRRRRQQPGLVATVEEA
                     QVGRIPPGMPRGWVRVMGCAFALVGLVSLGLAWAHHKQAIAKAVQLQPALRFDASFTM
                     PDQIGEWKRLPTEVPPLQKVETVGVRSQVWHYRLGDMQVSLALDYPFRGYHSLEGCYA
                     LSGWHLLERRSGGGSGTNGSLPYVEVHMQNNMGMHGILWFSLVDERNRWLTGAAYVPT
                     LSERLRQRIQLGGESEFVSYQIQVLATGFNPMRPAEQEQVLRFFKEGRRILWQQLSGQ
                     IQRQT"
     gene            5799..7142
                     /locus_tag="PKI07_12315"
     CDS             5799..7142
                     /locus_tag="PKI07_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51868.1"
                     /translation="MAVWLAFGIYLLAGKPWQTQAHYTRIAAAALAAKDFETARIASQ
                     RLLALGVEPRRKYLFDLALALGGLGRGKEAVSLLGNIAPDDKPGYLPAHLFVAQTLLV
                     KTNATPVEMRIAETHLKHAIALDPNSPAANELLGRLYVRSGRWELAEKHLRVIVSAKP
                     EISLLLAAAHRARGDNLAARNWAERAAKYHRDKVQASKLDLPSDRLAWADAMAMTEDY
                     ATASLILEAGWQQFGNQAYLQPLGEVCALWLKSIAQTRPGDLASQITLIERGLECAPQ
                     NETLLRHLIRLTHLEGPEAAAARHTLKRMLAEGKAAATLHFALGVDAWQQGHPEEALK
                     HLELAYAAAPLLPQVANNMAMILTVGNKPDLPRALMIIESVLQKFPQDPVFRQTRGEI
                     LVRSGRSQAAIADLEYALPRLASKRATHAALAEAYRAVGLRDLATEHERMAKTDR"
     gene            complement(7161..8777)
                     /locus_tag="PKI07_12320"
     CDS             complement(7161..8777)
                     /locus_tag="PKI07_12320"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOC51869.1"
                     /translation="MNYWPTISCALLGMGIVMGIVPLILRPTVLAKLSHRWRDLHHTH
                     LKPIPRVGGLALAVAFLGVELLAMVMWPGQTQKLPVRWVVLLSSLAMFMLGFWDDLKP
                     LGAKRKLLGQVLIATAVCAFGVEIQQFTVPFSATILPLGGWGTLLTIFWLVGMTNLIN
                     LIDGVDGLAAGICLMLMVLLAYVGHQGGSFGLLASGMAGALLAFLWFNFPPARIYLGD
                     GGAYFLGFQIGLFAIVSSHKGTVFAALIAPLFALALPIVDTVLAILRRGLRGLPIFRP
                     DRRHIHHHLLNMGLSRRKVVLSIYAVTLVFMLMGFVAFWSHGNLIPVLLGVATLILLI
                     CAGKLHFSRNWFAVGRVVGNSLEMRQEIQYALTLMHWLAFEGKRAHSVEELWMDLVFA
                     AQRLGYNSVRMTLADGERAWQQADAASSTHRAVHTLQGGRFGTLELKAVVCLDAKYGA
                     ESPLKCGKRFCPCVSSAKVFEIVSELLAEGWVKGLSALKMNETAPARFDDQPAVAVNR
                     PHPRHSIFSNSAPAPAAHRQPDISQAGTSR"
BASE COUNT         1866 a         2258 c         2938 g         1925 t
ORIGIN      
        1 cggcaccggt cactccgggg acgtatacaa cggattggcg gatggtgcgc gatggttacg
       61 cctggtttgg gccgacgcta accaggcaag tgaccgtcgg cagtggcagc ggttcttgct
      121 gcggcgggag cgataacaat cggggcagcc tgccgcctgc gaattggaca cacgacaccg
      181 gggaattcaa tccatcgtta gaaggaacgt ggtcttcggc actgaactgt ggcggggccg
      241 attattggta tacgggccgc ctttcgggca cgcgcaacgc gtggtgggat gcggggtggg
      301 attttcgggg ccgttacgcc tttgattttc tcattctcgc ttgccagggc gcttggcgcg
      361 gggtgcccaa ttaccgctgc taccggcgcg acgggagcga gattctgggc tggggggtta
      421 atcaatgcga ccagaatggc tggtggaatt tctggacggg caactttaat gggaacagcg
      481 atcggttttg gcgatttcgt attcggggag aggattcggc gaaatgcggg acggcgaaca
      541 cgattgttgc ctgtcgcgcg cgtttctacg gagcgaagtg gtactacata aacgactggg
      601 tttgcctggg cggctacagt tccagttccg ttagcgacac gcatggcttt gacgaagcca
      661 atgcgtatct ctacccggct atagatacga gtcatggcaa cgtgattgcc ccggccctgt
      721 tcaacggcaa ggttccaggc cgcgtaaaga ccggcgattg caatcatgcc aacagcctca
      781 acttcaaggg caacgccgca agctacgggg gcggcgacaa catggattcc tacggctttg
      841 cctgggctta tgcgcccgag ggcgcgggtc ccaagtttct gattggctcg gatgacggta
      901 atcgggtgtg ggtcaacggc acccggatta acgacaataa cgcataccga gggctcacgc
      961 gcgatgcgga tgagaccggg ggaatcggct ttcagaaggg atggaatcgg attctgttca
     1021 aagtgcataa tggcggcggt ggatttgagg ggacgataag cttgcggaat ggaggaaccc
     1081 gtgattggaa cgagccaagt gtgaccgtct tcgatatggg aaacggggtt tatagttttg
     1141 gcgcgggttt tgagcaggac tggtggtatc cgtgcatcgg cgtggcgaat ttctatggcg
     1201 cgagcaatcc gcagcccaac agcaatcatt acggcaataa taccaccgtc gccgccagcg
     1261 gcagtgcgca gggctgcggc ccggtgcctt tttggaaggt gatgcagttt cagtggggat
     1321 acggcatccc ggtgagcaat tacgcggacg tgagcgccag cggaacgagt tggtcgcaca
     1381 cccaaaccgg ggtgaccggg catcggcggt ttcacttttt cgcggtcagc cggtccgggc
     1441 gaacttcatt ccagaacagc ggcgtgagcg ggggatggaa ttgggtcggg ggcgggtcgg
     1501 ggaactatat ggatgtgtat gtggacaatg tggcgccggt taatccgagc ttcacaaccg
     1561 cctctgccgt gagcaccacc cagattaacc tggcttgggc gattcccctg gatcaggggg
     1621 tgggcattgt ggccggagcg acggaggccg ctgatgagac gtccgattcc tccgccaatt
     1681 actaccggcg gggcgacgtg ggagtgcagg tatatcggga tgccgtggcg atttcggcct
     1741 ggggcgttgg gacctcgaaa aatgacaccg gcttgacgcc caataatccc tacacctaca
     1801 cgatagaggc gcgggataat aacagccaaa gccgcggggc gtgggccaac accaccgggc
     1861 ggcaaggcgc gacggttgtt tggaccctgt caatgccacc cgtggcgggc agtgtggtgc
     1921 cggacaattt aaccccttgc gcgggcagcc cggtgacgtg gtccgccacg gaaggcttcg
     1981 gggcgggcag tgttgccaag tacaaatatg cctggaatca aagcccgacg tatgcctgga
     2041 ctgggtccga gccggattgg tccgtggata ccctccagac cattcccgcg gttgcgggga
     2101 cgtggtattt gcacgtgaaa ggatataacg gagcgggggt ggagaatggg acccatgctt
     2161 atgcgataac gacgctggcg gcaccgacga cgacgggcat tgcgggcagc agcgcggtgg
     2221 cgatagggca ggtgggggag atttacacgg tgaccccgcc accggccagc ggatcgagtt
     2281 ttgtgtggag cgtgcccggg ggcgcggtga tcaccgccgg tggaagtggc ccgaacaata
     2341 gtcagattgt ggttacgttt ggaagttcga gcggagatgt gacggtgcgg gagacgacgg
     2401 cgggaggatg cgaagggccg ttggtgatga aaggggtgac ggtggggccg aatcgggcgc
     2461 ccgtggctgg ggcggttaag acgattagca cgacgaagaa cacaccggtg accatttaca
     2521 aggtgaaact gctggcgggg gcgacggatg ccgatggaga tccgttgagc atcagcgcgg
     2581 cggggccggt gagtgcggag ggagggcagg tggaagagcg ggctaatgat gtgaaatata
     2641 cgccgcaggt ggatttctgg gggccggaca gttttaccta tacgattagc gatggacagg
     2701 gggggacggc ggtagggact gtggaggtga tggtaagtta tggcggagga ttatcgccga
     2761 atttggtgta ttggccgacc catgatggca cgatgttccg ggtgacgttt gcggggatcc
     2821 ccggcaagga gtatggggtg gagtggtctg aagccccgtc cgggatgccg tggacatggt
     2881 tgaagaatgc gacggcggga ccaaacgggt tatttgaggt gacggatgtg gtttcggaag
     2941 tgccggcccg gtattatcgg atagtgtatc cgccgccgca gccgtagcag ccggggtgaa
     3001 aaaagtattt tctgttgacg cgacagtgtc cggtgcttta atatgcgaca taatttgatt
     3061 aagcccatga agctcaggtt tttggccatt ggtgtcgtgt gggccagcat gacgatgctg
     3121 gtgcagggag agtgggcgac ttttacatac agcagcggct tccagaatgg aggggtgatt
     3181 ccggacaatt cgctggtggg ttgggcggat acccggcagg tgagtggctt ggtcggcgaa
     3241 attctggacg taaacgtggg gctgcagttg agtggcggct ggaatgggga tctgtattcc
     3301 tggctggtgc atagcactgg ttttgcggtt ttgctgaacc gggtaggcgg cacgggggtt
     3361 aatcccccgg gttatggcga cgctggaatg agtatgatgc tggatgacca gggcgaaaag
     3421 ggagacattc attggtatgg aggtggtttt attcccacga cgactgcatg gccaactgtc
     3481 ccggacgggg ctccgacacc atcggataat tggaaaccgg acggacgtta tgttgacccc
     3541 atagatggag atgtgggggt tgcgccacgg accgcactgc tgagttcgtt taacgggttg
     3601 aatccaaatg gcgactggac gctgttcatc gcggacgttg cggctggcga aaccagtacg
     3661 ctaacaagct ggactttgaa cattcagacg gctgccgtgc cggaaccggc cagcctggtg
     3721 caaggcgcgg tggtggtgtt gttgatgggc ggagtgatgg ggggctatcg cttgagaggg
     3781 cagaagcggc ggtttcattc ccattgactc cggtcgctgg ttaacgatta gcttcggggg
     3841 catgtcagca gtaagggggt aaaggacagg ccggtttctt gcggggcgtt gatcccatgc
     3901 ggaccgtcac gggttgaggc ctcgtggcga gcagaggcta tggcggccat gcaaggtttt
     3961 ctgccgggtt ggccctcaaa tgcccaggaa aaggcgcttg attggtgcag ataacggcaa
     4021 gatacagttg tagcgaagtc gttcgggttc ggttagggtg tgacgagaaa agcgatgact
     4081 acggctggcg aaccaaatag ttcgagaaca gaagcccagc tttcggggga cgcacctgtg
     4141 gattctggaa taggttgggg ttacggtctg ctctttgtgc tgggctacgc tccgctgttg
     4201 gggttgtttt ttttgaactt gtggggacgc cctcattacc agttctttcc ctgggctttg
     4261 ctgggggcag gcttcctggc ttggatccgg gggaaggaat tgagtcgccc cttcgtgcct
     4321 ggggatcgca aggtgaccat gtttctgcag ggggtctcgt ttttactcct gggtttggct
     4381 acggtatcct ggtcgccatg gctggcctgt ctggcggccc taactggctt ggtggccgcg
     4441 atttgggggc ggggaggtag taagctgctg aatgcgatgc tgccggcact gatattgtta
     4501 ttaataatca tcccgccgcc actgctggct gacaaccttt tgattcaaca aatgcgcgtg
     4561 ttggcggttc gagggagcag cagcgtgctg gacagcctgg gagtgataca cgccctgagc
     4621 ggcaatgtca tcgagatacc cggccagcga ttattggtgg acgaagcttg cagcggcata
     4681 aattcagttt tggtgctcct ggcttgtgcg ctgttttacg gcctgtggcg gcggcgatct
     4741 ccgctgcaca tccttctgag tctggttaat gcgttgggga ttgtgctgtt gggcaacctg
     4801 gcccggatta cgttaggcgc ctgggtcaag tttcgttacg ggattgatat ccttactggg
     4861 acggttcatc aggtggcagg gttgcttctg ttcccgacct atatcggaat tattttgagc
     4921 atggatcagt tgctggcgta tctgttgaat ccaatccgac ggcgaagaca acagccgggg
     4981 ctggtagcca cggtcgaaga ggctcaggtt ggccggattc cgccggggat gccccgcggt
     5041 tgggtgcgag tcatgggatg cgcgttcgca ttggttggtt tggtgagcct ggggctggcc
     5101 tgggcgcatc ataagcaggc tatagcgaaa gccgttcaac tccaaccggc cttgcgcttc
     5161 gatgcctcct ttacgatgcc tgaccaaatc ggagagtgga aacggcttcc cacagaagtt
     5221 cctcccctcc aaaaggtgga gacagtgggc gtccgatcgc aggtttggca ttatcgttta
     5281 ggggatatgc aagtgtcgct ggcgttggat tatccctttc gtgggtatca ctctttggag
     5341 gggtgctatg ccttgagcgg ttggcatttg ttggagaggc gatctggtgg gggatcaggg
     5401 acaaatggca gcctgccgta tgtcgaagtt cacatgcaaa acaatatggg gatgcatggg
     5461 attctttggt tcagcttggt ggacgagcgc aaccgctggc tgaccggggc ggcttatgtc
     5521 cccactttaa gtgaaagact ccggcagcga atacaattgg ggggagaaag tgaattcgtc
     5581 agctaccaga tacaggttct ggccaccggg tttaatccca tgcgcccggc agaacaggag
     5641 caagtgttgc ggttcttcaa ggagggacgc cgaatccttt ggcagcagct ttccgggcaa
     5701 atccagcgcc agacatgaac attcgccgtt tctttaagcg ttgggccaag tcccgggagc
     5761 cccggcttct ggtatggggg attccggcgc ttgtgggctt ggcggtttgg ctggcctttg
     5821 gcatctacct gctcgctggg aaaccttggc aaacgcaggc gcactatacc cgaatcgccg
     5881 ctgccgccct ggcggcgaaa gactttgaga cggcacggat agcctcccag agattgttgg
     5941 cgctgggggt tgagcctcgg cgaaaatacc ttttcgacct ggctctggct ttgggaggtc
     6001 tgggccgcgg aaaggaagcg gtatcgttgt taggcaatat cgccccggac gataagcccg
     6061 gttatcttcc agcccacctc tttgtcgctc aaaccttgtt ggttaagacc aacgcgacgc
     6121 cggtggaaat gagaatagcc gaaacacacc tgaagcatgc cattgcgctt gatcctaatt
     6181 ccccagccgc taacgaactt ctggggcgct tgtatgtccg ttctgggcgt tgggagttgg
     6241 ccgagaagca cttgcgtgtg attgtgtccg ccaagcctga gatttccctc ttgctggcgg
     6301 cggctcaccg ggctcggggc gataatctgg cggcccgtaa ttgggcggaa cgcgccgcca
     6361 agtaccatcg ggataaggtg caggcgtcga agttggactt gccctcggac cggttagcct
     6421 gggcggatgc catggcgatg acggaggatt atgccactgc ttccctcatt ttggaagccg
     6481 gctggcaaca gtttggcaac caggcctact tgcaaccgct gggggaagtc tgtgccctgt
     6541 ggttgaaaag catcgcccaa acccggcctg gggatttggc ctcacagatc acgctgatcg
     6601 agcgcgggtt ggaatgcgcc ccgcaaaacg aaaccttgct tcggcatctc attagattga
     6661 cccacttgga ggggccagaa gcggcggcgg ctcgccatac ccttaaacgc atgctggccg
     6721 aaggaaaagc cgccgctacg ttgcatttcg ctttgggggt tgatgcgtgg caacagggtc
     6781 atccagagga agccctaaaa caccttgaac tggcgtacgc agccgccccg ctattgccgc
     6841 aggtggctaa caatatggct atgattctca ctgtcggaaa caaaccggac ctgccccggg
     6901 ctctgatgat tattgaatcc gttctccaaa agtttcccca ggaccccgtc tttcgccaaa
     6961 cgcgcgggga gattctagtg cgctccggac gttcccaggc ggcgattgcc gacctggaat
     7021 acgcactgcc gcggctggcc tcaaagcgcg ccactcatgc ggctctggcg gaagcgtatc
     7081 gtgcagtagg cttgcgtgat ttggcaacgg aacacgagcg gatggccaaa acggatcggt
     7141 gatcggtgtt tgcgattggg ctagcgggag gtgcccgctt gagaaatgtc aggctgccta
     7201 tgtgcagccg gagccggtgc gctattcgag aagattgaat ggcgaggatg tggacggttg
     7261 acggcaacag ctggctggtc atcgaatctg gcgggggcag tctcattcat ttttaaggcc
     7321 gagagccctt tgacccatcc ttccgccagc aattcactta caatttcgaa tactttagct
     7381 gaggacacgc aggggcaaaa tctcttgcca catttcaagg gcgattcggc tccgtatttt
     7441 gcgtcaaggc aaacgacggc tttcaattcg agtgtgccga agcggccgcc ttgcaaggta
     7501 tgcactgccc ggtgcgtgga actggcggca tcagcctgct gccatgcgcg ttcaccatcc
     7561 gccagggtca tccggactga attatacccc agtcgctggg cggcaaatac cagatccatc
     7621 catagttcct cgacggagtg ggcacgcttt ccctcgaagg caagccagtg catcagggtt
     7681 agggcgtatt gaatctcctg ccgcatctcc aatgaattgc ctaccactcg tcctaccgcg
     7741 aaccagttcc ggctgaaatg aagtttgccg gcacagatca gcaaaatgag tgtcgccacg
     7801 cccagcagca caggaatcaa attgccgtgc gaccaaaatg cgacaaaacc catgagcatg
     7861 aacaccagcg tcacagcgta aatcgagagt accaccttgc gacgggacag tcccatattc
     7921 aacaggtgat gatggatatg tctgcggtca ggtcggaaga taggcaagcc gcgcaagccg
     7981 cggcgaagga tggcaagaac ggtatcgaca atgggtaatg ccagcgcaaa taaaggggct
     8041 atcagtgccg caaagacggt tcctttatgc gaactgacga tggcaaacaa gccaatctga
     8101 aagcccagaa aatacgcccc gccgtcaccc aaataaatgc gtgctggcgg aaagttaaac
     8161 cagaggaagg ccagtagggc ccccgccatt cctgaagcca acaggccaaa actgccgcct
     8221 tggtggccca cataggccaa taaaaccatc agcatcaaac agattcctgc cgccagccca
     8281 tccacaccgt ctatcaggtt gatcaagttc gtcataccta ccagccagaa gatggtaagg
     8341 agcgtgcccc agccaccgag ggggaggatg gttgcggaga agggcaccgt aaactgctgg
     8401 atttccaccc caaaggcgca aaccgctgtc gcgattagca cctgtcccaa tagcttgcgt
     8461 ttggcgccca gcggtttcag gtcatcccaa aagccgagca taaacatcgc cagagaactc
     8521 agtaagacca cccacctcac aggaagtttt tgcgtctgtc ccggccacat gaccatggcc
     8581 agcaactcga ctcccaggaa ggcaacagcc agcgccagtc cgcccaccct cgggatcggt
     8641 ttcagatggg tgtggtgcag gtcgcgccag cgatgcgata gttttgccag cacagttggt
     8701 cgaagaataa ggggaacgat tcccatcact atccccatac ctaacagtgc gcaactaatg
     8761 gttggccaat agttcatcag gaaggcgtta ggcatcgaac gtggcgacag ggcatcgatg
     8821 cgatttgttt ttcctggggg gctggctcat tcgtgaagaa tccttcctgc agaataacat
     8881 ttcgtgcgcc gccccgcgcc agagctacta gttcgagaaa gcatccgttg caaccagtga
     8941 ttcagttcgc gagaaaccat acttcacttg tgttaaccgt tataaag
//
LOCUS       DAOPQG010000133         8910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_8817_length_8910_cov_12.902089, whole genome shotgun sequence.
ACCESSION   DAOPQG010000133 DAOPQG010000000
VERSION     DAOPQG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8817_length_8910_cov_12.902089"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKI07_12325"
     CDS             <1..180
                     /locus_tag="PKI07_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51870.1"
                     /translation="TDDLVLTKDALYQLSYMGSNQFKSNISSRYQKRQKPHCPAFPFE
                     KGRDTKVFDILRPDN"
     gene            complement(309..980)
                     /locus_tag="PKI07_12330"
     CDS             complement(309..980)
                     /locus_tag="PKI07_12330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC51871.1"
                     /translation="MRLPSPIHAVIFDLDGTLLDTLEDIGDAANRVLVSRGFPAHPIS
                     RYREFVGEGVARLIARAVPQPNPDPAMVQACIEAYQQEYARNWNVKTKPYAGVAGLLE
                     ALAARGLKLAVLSNKPDVFTRQCVRAFFPGRDFAEVMGASEAFPRKPDPASAREIARR
                     LGVTAAECLYVGDSGVDMQTARSAGMFAVGVLWGFRDRSELQREGAQALVQKPREILE
                     LLGEA"
     gene            complement(1033..2523)
                     /locus_tag="PKI07_12335"
     CDS             complement(1033..2523)
                     /locus_tag="PKI07_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC51872.1"
                     /translation="MRWTGVLLLLAAGQCASGAPAERPKPPNILVIMSDEHNASVLGC
                     YGNPIVRTPNLDRLAARGVVFENCYCNSPLCVPSRQSFTTGKYISRIGAWNNHCRAPA
                     DSPSLPRVLQAAGYETFLCGKMHYDAACRYGFTETGGNMNRSQMTGLGRRRPADELQS
                     APGYSDRFQDFRTGEASGPMKHDQAVTAGAREFLRQRQPGGKPFFLLVGYLAPHFPLI
                     VPEPYWAQYRGKVPLPEIPEGFLAGLPLNYQHLRVGFHYENVPPELVRKGRELYYGLT
                     EWMDAEVGKVLKALAASAAASNTVVIYTADHGENMGEHGLWWKNCVYDSAARVPLIVS
                     WPERWPGGQRRAGVCSLVDVVQTIADLGGARTPADWNGNSLAPVLANGGAPWKDRAVS
                     EYYAHHVASGYAMIRAGQYKYVYHAAPDARHPAQRELYDLQVDPGEFRNLALHPKQKA
                     RVEQLHAALVREIGEDPEVTEQRCRRDIAQGYGVATRGQKKRRAAE"
     gene            complement(2667..3554)
                     /gene="cysK"
                     /locus_tag="PKI07_12340"
     CDS             complement(2667..3554)
                     /gene="cysK"
                     /locus_tag="PKI07_12340"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOC51873.1"
                     /translation="MLTDNILDLIGRTPLVRLNGEFIFAKAEFLNPGGSIKDRVALAM
                     IEGAERDRRLRPDSIIVEPTSGNTGIGIALVGRLKGYQVRIVMPENMSEERKKLIRSL
                     GGELVLTPAAAGIGGAVEQVRRMQAEEARVFVPQQFENPDNPRVHYEETAHELWRQMN
                     GDIAAFVAGVGSGGTLQGVGQFLRERKPEVRIVAVEPRNVSALLGHEPGLHQIQGIGD
                     GFIPAVLDVKLVDEVIEVSDEDAIETTRGLGRDHGLLVGISSGANVWAARQLARRLGG
                     NIATILPDRAERYFSTALM"
     gene            4033..4893
                     /locus_tag="PKI07_12345"
     CDS             4033..4893
                     /locus_tag="PKI07_12345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC51874.1"
                     /translation="MPSPLKISVVTPSFNSIHTIRATIESVAQQDYPHVEHLVMDGGS
                     TDGTLEILKAYPRLLWVSEKDEGHYHAMDKGTRQASGDVVAILNADDCYRPGVLREVA
                     AAFEAHPDWDALFGNIVFVDGEGREIFRREEAKFDPQIVRFGHNMVNHQALFLKRAVY
                     LRLGGYRYREFKNCCDYEYVMRLIRAGCRIGHIPVYIVNYRYHDHGQSADLRVRANMA
                     RESAIIRKEYGMPGGVVGKALQTYARFKRQLEKLFILGKCDLIPGGRHLKRHLRAKTS
                     FSSNIGVDKL"
     gene            complement(4858..6108)
                     /locus_tag="PKI07_12350"
     CDS             complement(4858..6108)
                     /locus_tag="PKI07_12350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC51875.1"
                     /translation="MKSKARAGKVLMIAYACDPRGSGEHWLGWGWAEQASRSYEVELV
                     TTPKARAAVEESSRAAGIAPHFIDVPEWLRSLADRGWASWSRKLAWQKRVARLARALN
                     REKKLSLVHQTTFHTFRVPFLAAGLGIPSVWGPIAGGEHVPPGFGRYLGPARWTEKGR
                     TLVNRFWLQTPAIQRSLRQASVLYISNRTTLDFLPLRFQAKCRIVSPNALRPEDQQWV
                     CPPAEAAAGGAAFRMLYVGNCVPTRAIPLVLEALLQARLGDYELTVVGDGPALAHWKR
                     RAAELGLAANVRFAGKVPRAQLDAFYARADVLVFPALRDSGGSALLEAMARGVPVICL
                     DWAGPGEMVDETSGIKVPVSTPEAAVGAFAAALARLQREPALRTRLARAARARAEARF
                     GWDAKRQLLEETYLKLIHPDIGGE"
     gene            complement(6083..6811)
                     /locus_tag="PKI07_12355"
     CDS             complement(6083..6811)
                     /locus_tag="PKI07_12355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HOC51876.1"
                     /translation="MKFPLLKEWLSRGGFLVPPEVIHFANGVLNYLNVGRWFYDRKLK
                     VPVRCAGREPLYDYVAGFVKEPVSYLEFGVFKGATLRYWTQLLKHPDSFLHGFDSFEG
                     LPENRGLYVGKQVLNLDGVMPKFDDPRVRLFKGWFSDTLPNYVQAFRPHASLVLHLDA
                     DLYSSTIFVLRQMLPFLRPGTILIFDEFFDREHELKAFTEFLDEAGLAVECLAATRAL
                     TQAAFRITQIASQPAAHEVESARR"
     gene            complement(6818..8152)
                     /locus_tag="PKI07_12360"
     CDS             complement(6818..8152)
                     /locus_tag="PKI07_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC51877.1"
                     /translation="METHATLIIGAGLTGLSCAYHLAGDYLLVEREGEPGGMARTHER
                     HPGFLCDSTGHWLHLRSDYMKALVRKLLPDGLVEHGRKAVIHLCDTFTPYPFQANTYG
                     LPREVVLECLIGLLEARHPEDFGLSPAAAAPRDFREWIVRNFGEGIARHFMVPYNEKL
                     LGVRLEELRPEYAERFIPRPSLEDVVKGALGFSRESLGYNARFVYPREGGIGGLPRAI
                     ARGLQVPPVYNVAVTRVAPRRRVATLSDGRQVVYRRLLNTAPLPQFLSLLLDLPAEVQ
                     QAARQLRSTTVRYFDLGVRGPGDAASHHHWIYFPEPEYVFYRTGSYSAVHPGTAPPDC
                     RSYYVEMSGGAADLLKQPEQLKQRVLSDLRKARVLSSRDEVLFMKLCEIPFAYVIFDH
                     NYERSRQVILDYLADQGILSCGRWGGWGYGGMEDAMQDGKTSAEQITRGRTS"
     gene            complement(8264..>8910)
                     /locus_tag="PKI07_12365"
     CDS             complement(8264..>8910)
                     /locus_tag="PKI07_12365"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOC51878.1"
                     /translation="LVPPEEKDRALAVLVRDIEETHHGHLSTGIFGTRFMLHALADLG
                     RADTAFQVVNQRTFPGWGHMLENGATTLWEHWEFSDNTYSHNHPMFGSVSEWFYKVLA
                     GINAAPDAAGFDRILIRPQPVGGLRWVAASYDSVRGKVVSEWRREGGQFKLHIRVPVG
                     AVATVFVPARAGAEITEGGQPIARAAGVRLSGRENGSVVLNVAAGEYHFASPLE"
BASE COUNT         1787 a         3179 c         2428 g         1516 t
ORIGIN      
        1 actgatgacc tcgtccttac caaggacgcg ctctaccaac tgagctacat gggcagcaac
       61 caattcaaat caaatatttc atcccgatac caaaaacgac aaaaacccca ctgtcccgct
      121 ttccctttcg aaaaaggccg ggataccaag gttttcgaca ttttaaggcc cgataactga
      181 cacagactac gaaaccctca accccctgtc aacgggtttc caactttttt ttcattttta
      241 ttttccgggc atttccccct ccccaatggc cactttcacc gccggccccg gagatcccgc
      301 cagcgccctc aagcctcccc cagcaactcc aatatctccc ggggcttttg aaccagggcc
      361 tgcgcccctt cgcgctgcaa ttccgagcga tcccgaaatc cccacagcac ccccaccgca
      421 aacatccccg ccgaccgtgc cgtctgcata tccacgcccg aatcccccac ataaagacat
      481 tccgccgccg taacccccag cctccgggcg atctcacgcg cactggccgg gtcgggtttg
      541 cgcggaaacg cctcgctcgc tcccataacc tccgcaaagt cccggccggg aaaaaatgcg
      601 cgcacgcact gccgggtaaa aacatccggc ttgttcgaca acaccgccag cttgagcccg
      661 cgggccgcca gcgcttcaag caatccggcc actccggcat acggcttcgt cttgacattc
      721 cagttccgcg cgtattcctg ctggtaagcc tcgatacacg cttgcaccat cgccggatcc
      781 ggatttggtt gaggcacggc ccgcgcgatc agacgcgcca caccctctcc gacaaactcc
      841 cgataccgcg aaatcggatg cgccgggaaa ccccggctca ccagaacccg gttggccgca
      901 tcgccaatgt cctccagcgt gtcgagcaat gtcccatcca aatcgaaaat aactgcgtgt
      961 atcggtgagg gaagcctcat atctcaaatc aaacccaatc ccggcaaaac caggcgcgtt
     1021 tgaaccggcg cgtcattctg cggcgcggcg cttcttctgg ccccgggtcg cgactccgta
     1081 gccttgggca atatcccggc ggcaacgctg ctccgttacc tcgggatctt cgccaatttc
     1141 cctgaccagc gcggcgtgca actgctcaac ccgcgccttt tgcttcgggt gcaatgccag
     1201 gttgcggaac tcgcccgggt cgacctggag atcgtaaagc tcgcgttggg ccggatgccg
     1261 cgcgtccggc gccgcgtgat aaacatattt atattgaccc gcgcggatca tggcgtagcc
     1321 ggaagcaaca tggtgcgcat aatactcgct cactgcacgg tctttccagg gcgctccccc
     1381 gttcgccaac accggcgcca gcgagtttcc gttccaatcc gccggcgtcc gggccccgcc
     1441 caggtcggca atcgtttgca ccacatccac cagcgagcaa accccggcgc ggcgctggcc
     1501 gccgggccaa cgctccggcc aggacacgat taacggcact cgagccgccg aatcatacac
     1561 gcaattcttc caccaaagcc cgtgctcgcc catgttttcg ccgtggtccg ccgtataaat
     1621 caccaccgtg ttgctggctg cggcgctcgc cgccagagcc ttcagcacct tccctacctc
     1681 cgcgtccatc cactccgtca ggccgtagta caactcgcgc cctttccgca ccagctccgg
     1741 cggcacgttt tcataatgaa accccacccg caggtgctgg tagtttaacg gcaagccggc
     1801 gagaaacccc tccgggattt ccggcagcgg aacttttccc cgatactgcg cccaatacgg
     1861 ctccggcacg atcaacggaa aatgcggcgc caggtagccc accagcagaa aaaacggctt
     1921 gcctcccggc tggcgttgcc gaagaaactc ccgggccccg gccgtcaccg cctggtcatg
     1981 cttcatggga ccggaggcct cccccgtgcg aaaatcctgg aaacgatccg aataccccgg
     2041 cgcggattgc aactcgtccg ccggccgccg ccgccccagc cccgtcatct gcgaccggtt
     2101 catgttgccg ccggtctcag taaaaccata acggcaagcg gcgtcgtaat gcatcttgcc
     2161 gcagagaaac gtctcgtagc ccgccgcctg cagcacccgc ggcaacgagg gcgaatccgc
     2221 cggcgcccgg cagtggttgt tccacgcccc gatgcgcgag atatatttgc ccgtggtaaa
     2281 ggactgccgc gaaggcacgc acaacggcga gttgcaatag cagttctcaa acaccacccc
     2341 tcgcgccgcc aggcgatcca gattcggcgt gcgcacaatc gggttgccgt agcaccccag
     2401 cacggatgcg ttgtgctcat cgctcatgat aaccagaatg ttcggcggct tcggccgctc
     2461 ggcgggcgcg ccgctggcgc actggcccgc cgccagcagc agcagcaccc ccgtccatcg
     2521 catcccgccg aaccaccggc ccgcgaactt gcagccgtcc cggaatcccg gcgctgaccc
     2581 atccatcttc ataccgcgcc agcgtgccag cccgccccgc cgacggcaag ctccgccgcc
     2641 gctcccggcc agacgcccgc agcaccctac attaacgccg tgctgaaata gcgctccgcg
     2701 cggtcgggca ggatggtggc gatattcccg ccaaggcgcc gcgccagttg ccgcgccgcc
     2761 cacacgttcg ccccggaaga aattcccacc agcaacccgt gatcgcgccc cagcccgcgc
     2821 gtcgtttcta tcgcatcctc atcgctcacc tcaatcacct catccaccag cttcacatcg
     2881 agcacggcgg gaataaaacc gtcgccgata ccctgaatct gatgcaatcc cggctcatga
     2941 ccaagcaggg cggagacatt ccggggctcg accgccacaa tgcgaacctc cggcttgcgc
     3001 tcgcggagaa actggccgac gccttgcagc gtccccccgc tccccacgcc cgccacaaac
     3061 gccgcaatat cgccgttcat ctgccgccac aattcatgcg ccgtctcctc gtaatgcacg
     3121 cgcggattat ccgggttttc aaactgctgc ggcacaaaca cccgcgcctc ctcggcctgc
     3181 atccgccgca cctgctcgac cgccccgcca atcccggccg ccgcgggcgt gagcaccagc
     3241 tcgcccccca ggctgcgaat cagcttcttg cgctcctcgc tcatattctc cggcatcaca
     3301 atccggactt gatacccctt gagccgcccc accagcgcga tcccgatgcc cgtgttgccg
     3361 gaagttggct cgacaatgat ggaatcgggc cgcagccggc gatcgcgctc cgccccctca
     3421 atcatggcca gggcaacgcg atccttgata ctcccgcccg ggttcagaaa ctccgccttg
     3481 gcaaagataa actcgccgtt caaacgcacc aacggcgtgc gcccaatcaa atccagaata
     3541 ttatcagtca gcatgaaacc agcgcggatt cttggccctg cccgcgccgg gagcaagccc
     3601 gaacccatcc cggcgtctga tcctgccgcc gtgaacactc cacctcaacc cgaacggcag
     3661 ccgccgaaag cacccgcccc gcgacggcct tggacagcgg tcgcgcggca cggtcgaaag
     3721 ccagcccgag gcggcgttat gcttttgacc gccaaccagc gtgcggcaga gcttctcgaa
     3781 ggtgccccgg gttgacgcgc ctttgccgaa ccgtcccaca ctcaaagcgc ccaaacatgc
     3841 cgccgcagtt cccgggacga caatcccttc accctcaaaa cctgcccaac cctccatggg
     3901 cttccgtaaa aagccaaatc ttttccccct catccacgaa acttaggccc gagaacaaaa
     3961 gaccctgatg cggctcgcga acacttgcca tttgaccttt tccggcgtac ctgacagctt
     4021 ttagcggtcg ctatgccgtc gccattgaaa atctcggtcg tcacgcccag ctttaacagc
     4081 attcacacga ttcgcgctac gatcgagagt gtcgcgcagc aggattaccc tcacgtcgag
     4141 cacctcgtaa tggatggcgg ctcgacggat ggcaccctcg aaatcctcaa agcctatccc
     4201 cgcctcctct gggtttcgga gaaggacgaa ggccattatc acgcgatgga caaaggaaca
     4261 cgccaggctt ccggagacgt cgtcgccatc ctcaacgcgg acgattgcta ccggcccgga
     4321 gtgttgcggg aagtcgcggc ggcttttgaa gcgcatcccg actgggatgc cttgtttggc
     4381 aacatcgtgt tcgtggacgg cgagggccgg gagatttttc ggcgcgagga ggcgaagttt
     4441 gacccgcaga tcgtccgctt cgggcacaac atggtcaatc accaggccct gtttctcaag
     4501 cgagcggttt atctgcggct cggcggctat cgctaccggg agttcaagaa ctgctgcgac
     4561 tacgaatatg tgatgcggtt aatccgcgcc ggttgccgca tcggccatat ccccgtgtat
     4621 atcgtcaatt accgctacca cgatcacggc cagtccgccg acttgcgggt gcgggccaac
     4681 atggcccggg agtccgccat catccgcaag gaatacggca tgcccggcgg ggttgtcggc
     4741 aaagccttgc agacctacgc ccggttcaaa cgccagctcg aaaagctttt tatcctcggg
     4801 aaatgcgacc tgattcccgg cggacggcat ctcaaacggc acctccgggc caaaacctca
     4861 ttctcctcca atatcggggt ggataagctt taggtaggtc tcctccagca actgccgctt
     4921 ggcgtcccac ccgaaccggg cttccgcgcg cgcgcgcgcc gcccgggcca ggcgggtgcg
     4981 caaagccggc tcccgctgca gccgggccag cgccgccgca aaggccccaa ccgccgcctc
     5041 cggcgttgag accggaacct ttattcccga agtttcatcc accatctccc ccggcccggc
     5101 ccagtccagg cagatgaccg gcaccccgcg cgccatggct tcgagcaggg ccgagccgcc
     5161 gctgtcgcgc aaggccggaa atacgagcac gtcggcccgg gcataaaaag cgtcgagctg
     5221 cgcgcgcggc actttgccgg caaaccggac attggccgcc agccccagct ccgccgcccg
     5281 ccgtttccag tgcgccagcg ccgggccgtc cccgactacc gtcagctcat aatcccccag
     5341 ccgcgcctgc agcaacgcct ccaacaccag cggaatggcg cgggtgggca cgcaattacc
     5401 cacgtaaagc atccgaaacg cggccccccc ggcggcagct tcggcggggg ggcaaaccca
     5461 ttgctgatct tcggggcgca aagcattcgg agaaacaatc cggcacttgg cctggaagcg
     5521 gagcggcaga aagtcgagcg tcgtgcgatt cgatatataa agcacggagg cctgccgcag
     5581 cgaacgctgg atggcagggg tctgcagcca aaagcggttc accagagtcc gccccttttc
     5641 ggtccatctt gccggcccga ggtaacgccc aaatccgggc ggcacatgtt cgccgccggc
     5701 aatcggtccc caaaccgatg gaatgcccag ccccgccgcc agaaagggca cccggaaagt
     5761 gtggaaggtg gtctgatgca ccaaggaaag cttcttttcc cggttcagcg cgcgggccag
     5821 ccgcgccacc cgcttctgcc aggccagctt gcgcgaccag ctcgcccacc cccggtcggc
     5881 cagcgaccgc agccactccg gcacgtcaat gaaatgcggc gcgatgcccg ccgcgcggga
     5941 gctttcctcc acggcggcac gcgccttggg ggtggtcacc aactccacct cgtaactgcg
     6001 cgaggcctgt tccgcccacc cccaccccag ccaatgctcg ccgctgccac ggggatcgca
     6061 cgcataggcg atcattaaaa ccttacctgc gcgcgctttc gacttcatgt gcggcaggct
     6121 ggcttgcgat ttgcgtgatc cggaaagccg cttgagtgag agcgcgtgtg gccgcgagac
     6181 attcaacggc gagcccggct tcatcgagga attccgtaaa tgccttcagt tcgtgttcgc
     6241 gatcgaagaa ctcgtcaaaa atgaggatcg tgcccggcct cagaaatggg agcatctgcc
     6301 gaagaacaaa tatggtcgaa ctgtaaagat cggcgtccag gtgcagaacc agactggcat
     6361 gcggtcgaaa cgcctgcacg taattcggca acgtatccga gaaccatccc ttgaacaacc
     6421 ggacgcgcgg gtcgtcaaat ttcggcatga cgccatcaag attgagaacc tgcttgccga
     6481 catacaggcc ccggttctcc ggcaggcctt cgaagctgtc gaatccgtgg aggaaggaat
     6541 ccggatgttt cagcaattgg gtccagtaac gcagcgtggc gcccttgaat acgccaaatt
     6601 ccagataaga aaccggttcc ttcacaaacc ccgcaacgta gtcatagagc ggttcccgtc
     6661 cggcacaccg aaccggcacc ttcagcttcc gatcgtaaaa ccaccgcccg acattcaggt
     6721 agttcagaac gccgttggcg aagtgaatta cctccggcgg caccaggaag ccaccccggc
     6781 tgagccactc ctttagcaga ggaaacttca tggtgcgtca actggttcgg ccccgagtaa
     6841 tttgctcggc tgaagttttc ccatcctgca tggcatcttc catcccgccg tagccccacc
     6901 cgccccaccg gccacaggaa aggatgccct ggtctgccag gtaatcaaga atgacttgcc
     6961 ggctccgctc gtagttgtga tcgaaaatga cgtaggcgaa tgggatctcg cataatttca
     7021 tgaacaggac ctcatcccgg ctcgaaagca cccgagcctt ccgcagatcg gacagcacgc
     7081 gttgcttcaa ctgctcaggc tgcttgagca aatcggcggc cccgcccgac atctcaacgt
     7141 aatagctgcg gcagtccggg ggcgcggtcc ccggatgcac tgcggaataa gagccagtcc
     7201 ggtagaaaac atactcgggc tccgggaaat aaatccaatg gtgatgactg gcggcatccc
     7261 cgggaccgcg cacgcccagg tcaaagtaac gaaccgtggt cgagcgaagc tggcgggcgg
     7321 cttgttgaac ctctgccggc agatccagca gcagcgaaag gaactgcggg agcggcgcgg
     7381 tgttgagcag ccgccggtaa accacctggc ggccgtcgga cagcgtggca acgcggcggc
     7441 ggggagccac ccgggtcacg gcaacattat agaccggcgg gacttggagc ccccgcgcaa
     7501 tcgcccgcgg caggccccca atgccccctt cgcgaggata gacaaaccgg gcattatagc
     7561 ccagcgactc gcgcgaaaag cccagcgcgc ccttgacgac atcttccagc gagggccgcg
     7621 ggataaaccg ctcggcgtat tccggacgca actcttccag ccgcacgccc aggagtttct
     7681 cgttgtacgg caccatgaaa tgccgggcga tgccctcgcc aaagttccgc acgatccatt
     7741 cccgaaagtc ccgcggcgcg gccgccgccg ggctcaggcc aaaatcttca gggtgccggg
     7801 cctcgagcaa tccgatcagg cattccagca ccacctcccg gggcagcccg taagtattgg
     7861 cctgaaacgg gtaaggagtg aacgtatcgc acaggtggat gacggctttt cgtccgtgct
     7921 cgacgagtcc gtcaggcaga agcttccgca ccagcgcctt catataatcg ctccgcaggt
     7981 gcaaccagtg ccccgtgctg tcgcatagga agcccggatg acgctcatgc gtgcgggcca
     8041 tgccgcccgg ttcgccctcg cgctccacga ggagataatc ccccgccaga tgatacgcgc
     8101 aggaaaggcc ggtcaggccc gccccgatga tcagtgttgc atgtgtttcc acaaaacccg
     8161 gttgtccgat gagtcgcttg ccaattaacg gcagttgatc cgcagcctac ctatcccaag
     8221 cgcccaggaa aagcttttat cgcagccgcc gcgccgcgcc ggctcattcg agcgggctgg
     8281 cgaagtgata ttcacccgcg gccacgttca gcacgacgct cccgttctcc cgcccggaaa
     8341 ggcgcacgcc cgcggcgcgg gcaatcggct ggcctccctc cgtgatttcc gcgcccgccc
     8401 gggccggcac gaaaaccgtc gccacggcgc cgaccggaac ccggatatgg agcttgaact
     8461 ggccgccttc ccttcgccac tcgctgacca ctttcccgcg cacggaatca taactcgcgg
     8521 ccacccagcg caggccgccc accggttgcg gccggatgag gatccgatca aagcccgcgg
     8581 cgtccggcgc ggcattgatg cccgccagca ctttgtagaa ccactcgctg accgagccga
     8641 acatcgggtg gttgtgcgaa taggtgttgt cgctgaactc ccaatgctcc cacaacgtcg
     8701 tcgcgccgtt ctccaacatg tggccccagc ccggaaaggt gcgctgattc accacctgga
     8761 acgcggtgtc cgcccgcccc aggtccgcca gcgcgtgcag catgaaccgg gtgccgaaga
     8821 tgcccgtgct caagtggccg tgatgggttt cttcaatgtc ccgcaccagc accgccagcg
     8881 cgcggtcctt ttcttccggc ggcaccagcc
//
LOCUS       DAOPQG010000134         8874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_8855_length_8874_cov_13.811543, whole genome shotgun sequence.
ACCESSION   DAOPQG010000134 DAOPQG010000000
VERSION     DAOPQG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8855_length_8874_cov_13.811543"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PKI07_12370"
     CDS             complement(<1..663)
                     /locus_tag="PKI07_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005201 - extracellular matrix structural
                     constituent [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmcB family cysteine-rich outer membrane
                     protein"
                     /protein_id="HOC51879.1"
                     /translation="MTFSATAILAVGVMAPATKLCAQEQLPQEQWNEQYRSEYDVPPG
                     KYYSGRSPFMRGQPKFQPVPVIAAAPAPAPAPAPPPPPPPPKATCSDPTGGLIKLGKT
                     MPAEATLGREFMAELNLTAQACAANVVVRDTVPANASYVRSEPAATVNGKQLTWQIGD
                     LEAGAARKIKLWLKAEQEGEIVNCASVFAEPRTCASTRVVHPAIQLVKKAPAEVVICD
                     PIP"
     gene            complement(963..2045)
                     /locus_tag="PKI07_12375"
     CDS             complement(963..2045)
                     /locus_tag="PKI07_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOC51880.1"
                     /translation="MKHGLNINKTGALDFLSLGALVHRLDPGIIPFRKATQCQIHVSG
                     GEFNVAANLADCFGLQTGIATAMVDYPIGDLIAERVRAMGVKPFYKRFKHDGVHGPNM
                     AAVYSDRGQGVRAPVVFYNRNNEAGAQLKPGDFDWAAIFAGGIRWFHSGGIFAALSPT
                     TAELIIEGMTAARKAGAIVSFDLNYRPKLWNISGGHDRAVEVLDRIVRQVDVLVGNEE
                     DLQLGLGIPGPAVAARNRLDPSAFLGMIDKVIQKHPQIKVVATTLREVHSTNRHSWSA
                     VAWVNGQTYTAPTCELDVFDRVGGGDGYAAGFFYGLLNGEDPGEAVKLGWAHGALLTT
                     FPGDTTMATVEQVRAFACGGSARIQR"
     gene            3153..4571
                     /locus_tag="PKI07_12380"
     CDS             3153..4571
                     /locus_tag="PKI07_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC51881.1"
                     /translation="MAYVFTRGPLPRGWVEWKRWRPHPARLGLAALTLAAGLFASTVW
                     AQQGGFSVTATTPADGAAHVPVEANIIVKFSAPVDPASLTPQTVQVNDQPLAELENGG
                     LAFPEADSAVVLFRMKANTVYQITLAGSVKDTQGNALGQPFSWRFASVSRIGQPGAPV
                     KTFARYPRHNVYGVPVNAPITLSFTTELEGKSLSGESVRVVPSSGGPAVPGKVAVEGR
                     RVIFRPEAPLAPNQTYEVEIAAGVKSKAGMTSERSSSWQFTTGDGPREGPVITECWYE
                     SYPEATGLRLVFHVAAENLLKEGAKADKAARATRMPGANGVLQAAVVSLAGLVAQQPL
                     STPTSRQAKDMPVTNGGPETVVYAAYTHGGGRGPGNSVAGYDGKDPGMELVNAAVAAA
                     LDGAGAVTLYDVGDLVEHGDEVRGDGVYSGRLKLSPQFPTGPALIAFSILRPDGSRTS
                     PETMSLYILPPPAAVESAGETQ"
     gene            4587..>8874
                     /locus_tag="PKI07_12385"
     CDS             4587..>8874
                     /locus_tag="PKI07_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOC51882.1"
                     /translation="MQQIIFPWLVGFWGWKKGTMKKARYALQRASRLAVGILTAAVFL
                     ADTVAVQAGGAWSSCTRTTSAGGWGAYAGPCYCGTMWLGWGGNGTDRCRWSTGSAGVA
                     RDMYLTYRHAFGKRSNGDYSADQWSYAEGSYNGSYAVDQSGSGCVESTLNTRASYNGD
                     DWLEVGSDGGGGRDTVADWAKFYPEYDAVYSSKSVVTDMYLGNVYQWRYSMSNPDSAW
                     WSWGNGVRFAHTGGQTMGPGWFSSDRVNNNPGTGPGGTMTVYFNMRPTVAGNHNEDFQ
                     MVAENTSWFGDRWNFSNGNMRTAVSNTRKPPGATDGWDDGGKKKGQTVRFSVAGADSW
                     GTGDSYSWDWDGATTQGWSAQNACTLTQEDANDRLKIAITGADPYCHSSGGLAINAST
                     CRYLTIRMAANGGSDAQFFWYSSAGGWSGERHVDWNIIPDGQMHVYRVALPASWSGTV
                     YQVRLDPVGTGAANGQTVYVDWLRVHHVSDGLSGKIWLGTVHGTWDLYESSAMTWDSG
                     TASWYKDYTIAHGYPQTYYACVRMFNNNAQESDNHDRRFGGYGGGTGYRSYKVLNTDP
                     TAANTSFPGNEWSSDNTPTLQWSYADTDANPQSNYRVQVAAAMDFASPVADTQFVAGP
                     GTACELGSPLANGQWYYRVGVLDGLSQTARNGTYTGGVTLGQAGAVTGGSSVSFDGTD
                     DHVVIPSFTDSFGSGMTVEFWAYPTASQNYARFMDFGTGPSNDNILAYRPGTSSDLAF
                     EVYNGAASGGTARAAGALINNEWHHYACVLQSDGTVAMYRDGTALSVRGKTAIPNVVS
                     RANNYLGRSNWAADSYYAGRMDEFAIYDRPLTQSEIQAHYNARTSATAYRDLVLQHSP
                     WAYYRMDDTSGTTAANLNGGLGWSTGTAFRVDAAPPTGVSLSIGAVTTDSIAVTGSGT
                     DAHSGVNAATGYNYSCTGKSDSGGKGTTHTWSGLSANTKYTGLRVTVSDQAVPTPNTA
                     ASADLSKYTLQNAAGTPTFSDVTETSMRVETTGPVNLTADNSGVIFHDGTADRAKVTE
                     LYDEVSGLTANTPYTFKAKALNGEGVATDYSATATQWTLSVPPGAGSVTSSGAPCLPN
                     TAVTWTAVGGFGAGRVQKYKYAWDQNPTHTWTEAESDWDGGTLQTQPTAEGTWYLHIK
                     GYNGANVGHGTYSYTVKVPRGAFQWVGSDGDWDFSSAGLWQDAEPAGVKYCDGRHVRL
                     DDQAATAAPVVALNTTVAPASVTNDSSKNYTIWGSGKISGSGRLTKEGSSTLTLGLGN
                     DYSGGTVVNAGTLLVTNLTGSGTGSGGVTVAGGTLGGDGIISGSVTVSANGTLAPGTG
                     AGVLGTLTLGAAPSLGGTVAMKIKKTSGPVYAADKLSLSAGELSYGGTLTVTELGGGV
                     PLAGGDVFELFDAADGFDADSSFSTLNLPALPTPPQGEPAVNWYTGHLTVD"
BASE COUNT         1691 a         2622 c         2993 g         1568 t
ORIGIN      
        1 cggaatcgga tcgcaaatca ccacttccgc cggcgccttc ttcaccaact gaatggcggg
       61 atgcactacg cgcgtgctgg cgcaggtgcg gggctcggca aagacggagg cgcagttcac
      121 aatctccccc tcctgctcgg ccttgagcca gagcttgatc ttgcgggcag cgccggcctc
      181 gagatcgccg atctgccagg tcagttgctt gccgttcacc gtcgcggccg gttcactgcg
      241 cacatagctg gcgttggccg gcacggtatc gcggacgacg acgttggcgg cgcacgcctg
      301 cgccgtcagg ttcaattccg ccatgaactc gcgccccagg gtggcctcgg cgggcatggt
      361 cttgcccagc ttgatcaatc cccccgtcgg gtcgctgcaa gtggccttgg gcggcggagg
      421 cgggggtggc ggcgccggag cgggagccgg agccggggcg gccgcgatga cgggcaccgg
      481 ctgaaacttc ggctggcccc gcataaacgg cgagcggccg gagtaatact tgccgggggg
      541 cacatcgtat tcgctccgat attgttcatt ccattgttcc tggggaagct gctcctgggc
      601 gcacagcttc gttgccgggg ccatcacgcc aacagccaga atcgctgttg ccgaaaaggt
      661 cataaacagg cctgtcctca tagtcagtgt ttgtctctat tattgtccaa cttacgttcc
      721 aatttacccg ctggatattc cgctcagcgg aacaccccaa ttatttatct cggtctggcg
      781 aggccagcgg cggatctcat tagcgcgcct actatagcat gagtttatca ccgcgcaagc
      841 cacggaatca aattgcttct ccggcaatgc ccggggaaac cgcccggccg cccggccccg
      901 gcggcggccc gtcccgccgg acgcccgccc gcccgcgcgc gaaaaagcgg ttcccggctc
      961 cgctatcgct gaatccgcgc cgagccgccg caggcaaagg cacgaacctg ctccaccgtg
     1021 gccatggtgg tgtcgccggg gaaggtcgtc agcagggcgc cgtgggccca gcccagcttg
     1081 acggcttccc cgggatcttc gccattgagc aggccataga aaaagcccgc cgcgtagcca
     1141 tcgccgccgc cgacgcggtc aaacacgtcc agctcgcaag tgggcgcggt gtaggtctgg
     1201 ccgttgaccc atgcaaccgc gctccagcta tggcggttgg tggaatgaac ctcgcgcagg
     1261 gtggtggcga cgaccttgat ctgcggatgc ttctggatga ccttgtctat catccccagg
     1321 aaggcgctcg ggtcaagccg gttccgggcg gccaccgcgg ggccggggat gcccagcccc
     1381 agttgcaggt cctcttcatt gccgaccagc acgtccacct gccgcacaat ccgatcgaga
     1441 acttccaccg cgcggtcatg gccgccggaa atgttccata gcttggggcg gtaattcaga
     1501 tcgaaggaaa cgatcgcgcc ggccttccgg gcggccgtca tcccctcgat gatgagctcg
     1561 gcggtggtcg gcgacaacgc cgcaaagatg ccgccgctgt ggaaccagcg gatgccgccg
     1621 gcgaaaatgg ccgcccagtc gaagtcgccc ggcttgagct gggctcccgc ttcattattg
     1681 cgattgtaaa acaccaccgg cgcgcgcacc ccctggcccc ggtcgctgta aacggcggcc
     1741 atgttgggac catgcacgcc gtcgtgcttg aagcgcttgt agaagggctt cacccccatc
     1801 gcccgcaccc gctcggcaat caggtcgccg atgggataat ccaccatcgc cgtggcaatg
     1861 cccgtctgca ggccaaagca atcggcgaga ttggccgcca cattaaactc gccgccgctc
     1921 acgtggattt ggcattgggt cgccttgcgg aaagggatga tgcccgggtc cagccgatga
     1981 accagcgcgc cgagcgataa aaagtccagc gcccccgtct tattgatgtt caatccatgc
     2041 ttcataataa agttatggcg ccattatgcc tcaaggaagg ctgttgtgtc tgctaaaaag
     2101 ttgacccggt tcaggctcaa gccggccagc aaaaaggcca ctctgccaaa ccacaaactg
     2161 catatcccag cggtttgcgc cctgaaaaat tgaatatctc cagccccaag ctcgcgcctt
     2221 gtccccttgc cctctccgcc tcgtcaaaag gaccggagcg ggtggccaaa gactgaaagg
     2281 agcacgttcg tcaaaacaaa ctcaaagcga gaaacagggt tcccacggca agaagcccgc
     2341 tcccctgcca aagctaaatt aaccccacac cctggctgcc atccctgccc gtctccccgg
     2401 ctaacgcctt ccttttcaac attctcattt ctgagaatcc tggccaactc ccccctccta
     2461 tcaggccatg tacaaggggt gtacaaggga tgctcaccgg aagtccatgg gattttagct
     2521 ggtacacccg gtgagcatcc cttgtacacc ccttgtacac ggctcttagc tagctatgga
     2581 aaggcttgga aacaggctgg ttatgctgga atacgggtgc ggccgagaaa aaggggggag
     2641 ggataggcaa aatgggcgtt ccgagggtcg gcgtggctgc tgatttagcg ccgggctggc
     2701 atggttgcga ctccgcagag gctattggag ctgacggaga gttttagcgg gtcaagcctg
     2761 gcgtagctcc attcccgggc ggcttccagg gccgattggg ctgccactct ggtaaatgtc
     2821 atttttgatg cggccagccc ttgacagtat gttagcaaaa gtgctaatat gcgcaacgta
     2881 ttactgatag tgacaaccca gacagagaaa tggcgcgtgg tgatcagaat gtctcccata
     2941 tttctccaat tcatggggcg ggaaggattg tatcgtcaat cagccctttt ccgcatgccc
     3001 tgccctgccg gggctgtctg cttgcccctg ccgaattaat ccttcgttca aacccagcaa
     3061 cgctatcgca aagttcacca atgaaacatt cgagaaccat caaggcaacc aatgagcgct
     3121 cgagggagct gaggccaggc tccgttgggc gaatggcata cgtttttacc cgcgggccgc
     3181 tgcctcgcgg ctgggtggaa tggaagcgct ggcggccgca tcccgcgcgc ctggggctgg
     3241 cggctctgac cctcgcggcg ggcctgttcg cttcgacggt ctgggcgcag caaggcgggt
     3301 tttcggtgac cgccaccacg ccggctgatg gggcggcgca tgtgccggtg gaagccaata
     3361 tcatcgtcaa gttcagcgcg cccgtggatc cggcgtcgct gacgccgcag acggtccagg
     3421 tcaatgatca accgctggcc gaattggaaa acggcgggct ggcctttccg gaggcggact
     3481 cggcggtggt gttgttccgg atgaaagcca acacggtgta tcagatcacg ctggccggga
     3541 gtgtaaagga cacgcagggc aatgcgctgg gccagccgtt tagctggcgg tttgcctccg
     3601 tgtcccgcat cgggcaaccg ggcgcaccgg tgaagacctt tgcgcggtat ccgcgccata
     3661 atgtctatgg ggttcccgtg aatgcgccca ttaccctgtc ctttacgacg gagttggagg
     3721 gaaaatcgtt gagcggggaa agcgtgcggg tggttccatc gtcgggcggg ccggcggtgc
     3781 cgggaaaggt tgcggtggag gggcggcggg tgatattccg cccggaagca ccgctggcgc
     3841 cgaaccagac ctacgaggtg gaaattgcgg cgggggttaa gagcaaagcg ggcatgacaa
     3901 gcgagcgcag ctcgtcgtgg cagttcacga ccggcgacgg gccgcgcgag gggccggtga
     3961 ttaccgagtg ctggtatgag agctacccgg aggcgacggg tttgcggctg gtgtttcatg
     4021 tggcggcgga gaacctgctt aaagaagggg ccaaggcgga caaggccgcg cgagcgaccc
     4081 ggatgccggg ggccaacgga gtattgcagg ccgcggtggt ttcacttgcc ggtttggtgg
     4141 cgcagcagcc gctgagcacg ccgacgtcgc ggcaggccaa ggatatgccg gtgaccaacg
     4201 ggggaccgga gacggtggtg tatgcggctt atacgcatgg aggcggccgc gggcccggca
     4261 acagcgtggc gggctacgat gggaaagacc cgggaatgga gctggtgaac gcggcggtgg
     4321 cggcggcgct ggacggagcc ggagcggtga ccttgtatga cgtgggcgac ctggtggaac
     4381 atggggatga ggtgcggggc gatggggttt actcaggccg gctgaagtta agcccccagt
     4441 ttccgacggg gcccgcgttg attgcgtttt caattctccg gccggacgga agccgtacca
     4501 gtccggaaac aatgtcctta tatattctgc cgccgcccgc ggcggtggag tcagcgggtg
     4561 agacgcaata agaaaggagc aagcgtatgc agcaaatcat ctttccctgg ttggttgggt
     4621 tttggggctg gaagaaaggc acgatgaaga aggcccgtta tgccttgcag cgggcttcgc
     4681 ggttggcggt ggggatattg accgcggcgg tgttcctggc cgacaccgtc gccgtgcagg
     4741 cgggcggggc ctggagctcc tgcacgcgca ccacttcggc gggcggctgg ggagcctacg
     4801 ccgggccctg ctactgcggg accatgtggt tgggctgggg cggcaatggc acggatcgct
     4861 gccgctggag caccggcagc gcgggcgtcg cgcgcgatat gtacctgacc tatcggcatg
     4921 cgtttggcaa gcgttcgaat ggcgattaca gcgccgatca atggtcctac gctgagggtt
     4981 catacaatgg gtcttacgcg gtcgaccagt ccggctcagg ctgcgtagaa tctactctga
     5041 atacgcgagc cagctacaac ggcgacgact ggctggaggt cggctcggat ggtggcggcg
     5101 gaagggacac ggttgccgat tgggccaagt tctacccgga gtatgatgcg gtttattcca
     5161 gcaagtcggt ggtaactgac atgtatctgg gcaatgtgta ccagtggcgg tattcgatga
     5221 gcaacccgga tagcgcgtgg tggtcctggg ggaatggagt tcgatttgcc cacacgggcg
     5281 ggcaaaccat gggcccgggt tggtttagct ccgatcgggt caacaacaac cccggcacgg
     5341 gcccgggcgg gacaatgact gtatatttca atatgcggcc cacggtcgcg ggcaatcaca
     5401 atgaagattt ccagatggtt gccgagaaca cctcctggtt tggcgacagg tggaacttca
     5461 gcaacggaaa catgcgcacg gcggtgagca acacccgaaa gccaccgggc gcaacggatg
     5521 gatgggacga cggcggcaag aagaagggcc agacggtccg cttttccgtt gccggcgcgg
     5581 acagttgggg cacgggcgat tcttactcat gggactggga cggcgccacg acgcaaggat
     5641 ggtctgccca aaacgcgtgt accctgaccc aggaagatgc caacgaccgc ttgaagattg
     5701 ccattaccgg ggctgacccc tactgccata gttccggcgg cctggcgatt aacgccagca
     5761 cttgccgcta cctgaccatt cgcatggcag ccaacggcgg ctcggacgcg caattcttct
     5821 ggtacagcag cgcgggcggc tggagcggag agcggcatgt ggattggaac atcattccgg
     5881 atggccaaat gcacgtgtac cgggtggccc tgccggcgtc ctggagcggc accgtttacc
     5941 aggttcggct ggatccggtc ggcaccggcg cagccaatgg gcaaacggtg tatgtggact
     6001 ggctgcgcgt acaccatgtc agcgacggcc tgtcggggaa aatctggctg ggcaccgttc
     6061 atggcacctg ggacctttac gagagcagcg ccatgacctg ggattcaggc acggcgtcct
     6121 ggtataagga ttacaccatc gcccacggct acccgcagac gtattacgcc tgcgtccgca
     6181 tgttcaataa caatgcccag gaaagcgaca accacgaccg ccggtttggc ggttacggcg
     6241 gcggcaccgg ctaccgctcc tacaaagtgt tgaacaccga cccgacggcg gccaacacca
     6301 gtttcccggg gaatgaatgg agctccgaca acaccccgac gctgcagtgg tcttacgccg
     6361 acaccgacgc gaaccctcag agcaattacc gcgtgcaggt cgccgccgcg atggattttg
     6421 cctccccggt ggcggatacc cagttcgtag ccggccccgg caccgcttgc gaactgggct
     6481 cgccgctggc caacggccag tggtattacc gcgtcggggt gcttgatggc ctgtcgcaaa
     6541 ccgcccgcaa cgggacgtac accggcggcg tgacgctggg gcaggcgggc gcggtgacgg
     6601 ggggctccag cgtgagcttc gacggcacgg atgaccatgt ggtgattccg agttttacgg
     6661 acagtttcgg cagcggaatg acggttgagt tttgggctta cccgaccgcc agccaaaact
     6721 atgcccggtt catggacttc ggcactggcc catcgaatga caatatcctg gcataccgcc
     6781 cgggcacttc gagcgacctg gccttcgagg tttataatgg cgcggcctcg ggaggcacgg
     6841 cgcgcgccgc cggcgccctc atcaacaacg aatggcacca ctatgcgtgc gtgctccaga
     6901 gcgacggcac ggtggccatg tatcgcgatg gaaccgccct gagcgtccgc ggcaagacgg
     6961 cgatccccaa tgtggtgagc cgcgccaaca actacctggg ccggagcaac tgggccgccg
     7021 attcgtatta tgccggccgc atggatgagt tcgccattta cgaccggccg ctcacgcaat
     7081 cggagatcca ggcgcactat aatgcccgga cttccgccac ggcctaccgc gaccttgtcc
     7141 tccagcatag cccctgggcc tactatcgca tggatgacac cagcggcacc accgccgcca
     7201 acctcaacgg cggccttggc tggtccaccg gcacggcttt ccgcgtggat gccgccccgc
     7261 caaccggggt gagcctctcc atcggcgctg tcacgacgga cagcattgcc gtgaccggct
     7321 cgggcaccga tgcgcattca ggggtgaatg cggccacggg ctacaattac agttgcaccg
     7381 gcaagagcga tagtgggggc aagggcacga cgcatacctg gagcggcctg agcgcgaaca
     7441 cgaaatacac ggggctgcgc gtcacggtca gcgaccaggc ggtgccgacg cccaatacgg
     7501 cggcctcggc ggacttgagc aagtacacgc tgcaaaacgc ggcggggacg cccaccttca
     7561 gcgacgtgac ggagacgagc atgcgggtgg aaacaaccgg gccggtcaac ctgacggcgg
     7621 acaattcggg ggtgattttc catgatggca cggccgatcg cgcgaaggtg acggagttgt
     7681 atgatgaggt gagcgggttg acggccaaca cgccctacac cttcaaagcc aaggcgctca
     7741 acggcgaagg cgtggccacg gactattcgg ccacggcgac gcagtggact ttgtcggtgc
     7801 cgccgggggc cgggagcgtg acgtcttcgg gggctccctg tttgcccaat acggcggtga
     7861 cgtggacggc ggtgggcggt tttggggcgg gccgggttca gaagtataag tatgcctggg
     7921 atcagaatcc cacccatacg tggaccgagg cggagagcga ctgggatggc ggcacgctgc
     7981 agacccagcc gacggcggag gggacgtggt atttgcatat caagggttac aacggggcca
     8041 atgtcggcca tggcacttac agttacacgg tgaaggtgcc gcggggggcg tttcagtggg
     8101 ttgggagcga cggggattgg gatttcagca gcgcgggctt gtggcaggat gcggagccgg
     8161 cgggggtgaa gtattgcgat ggccgccatg tgcgcctgga cgaccaggca gcgacggcgg
     8221 cgccggtggt ggcgctgaac acgacggtgg cgcccgccag cgtgacgaat gattcgagca
     8281 agaactacac gatttggggc agtggcaaga ttagcggcag cgggcgtttg accaaggagg
     8341 gcagcagcac gctgacgctg ggcctgggca atgattacag cgggggcacg gtcgtgaatg
     8401 ccggcacgct gctggtgacc aacctcacgg gcagcggcac gggcagcggg ggagtgacgg
     8461 tggcgggggg caccttgggc ggcgacggca tcataagcgg cagcgtcacg gtgagcgcca
     8521 acggcacgct ggctccgggg acgggggcgg gggtattggg cacgctgact ttgggcgcgg
     8581 cgccgagttt agggggcacg gtggcgatga agatcaagaa gaccagcggc ccggtttacg
     8641 cggcggacaa gctgagcctg agcgcggggg agttgagcta cggcggcaca ctcaccgtca
     8701 ccgagttggg gggcggggtg ccgctggcgg gcggggatgt attcgagttg tttgacgcgg
     8761 cggacggatt tgatgcggac agcagtttca gcacccttaa cctgccggcg ctgccaaccc
     8821 cgccgcaggg tgagccggcg gtgaactggt atacgggcca cctgacggtg gacg
//
LOCUS       DAOPQG010000135         8356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_9520_length_8356_cov_15.852548, whole genome shotgun sequence.
ACCESSION   DAOPQG010000135 DAOPQG010000000
VERSION     DAOPQG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9520_length_8356_cov_15.852548"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKI07_12390"
     CDS             <1..230
                     /locus_tag="PKI07_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51883.1"
                     /translation="IQGKSKVRRSRYYSGFASILSLHLRKPLILRAKGSGFGSHALES
                     AKKGARRGCGLGGQVRPGAPVERGRVDAMPG"
     gene            507..878
                     /locus_tag="PKI07_12395"
     CDS             507..878
                     /locus_tag="PKI07_12395"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC51884.1"
                     /translation="MKGFLNITKALADENRLRLMMALQGGEVCVCQLTELMGLAMSTV
                     SKHLSVLYQAGLVKARKEGRWMYYSLPGKGAPAAARGAVVWARRSLAGKEQVAADARR
                     LKQVLAMDINALCRRTRGSRS"
     gene            913..2244
                     /locus_tag="PKI07_12400"
     CDS             913..2244
                     /locus_tag="PKI07_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOC51885.1"
                     /translation="MNSITFQAGAGILNRELKVFGGFLAVFGLVFYLPVGVARFDNAL
                     QEGLALTKGYAREHVLLCLVPALFIAGGIGTFVSQASVMRYLGPKANPALAYGVASVS
                     GAILAVCSCTVLPLFGGIWMRGAGLGPATAFLYSGPAINVLAVVLTARVLGLELGVAR
                     AIGAVSFSVIVGLVMHGVFRREEQAKAAAAAAMPAVAARRPLWQTVLFFGSMVGVLVF
                     ANWSPPAEPAGLGAAIHAWKWPLTGGFAAGLGLSLGLWFGFKWRELTLAAMPVGLLSL
                     VFPHSPLAAFITGCAALAVLCARNEGEGREWLDATWTLGKQIFPLLLAGVFLSGILLG
                     RPGHEGLMPSEWVSRSVGGNSLRANFFSSVVGAFMYFATLTEVPILQGLIGAGMGRGP
                     ALALLLAGPALSLPSMLVLRSILGTRKTIVYVALVVVMAALAGLAGGVWLK"
     gene            2319..2558
                     /locus_tag="PKI07_12405"
     CDS             2319..2558
                     /locus_tag="PKI07_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOC51886.1"
                     /translation="MKILVIGPGCAKCQALAQLTEQAVKELGVAAEINKMTDLKQIMA
                     LGVMMTPALAVNGTIKVSGKVPRLAEIKAILQSAA"
     gene            2731..3213
                     /locus_tag="PKI07_12410"
     CDS             2731..3213
                     /locus_tag="PKI07_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOC51887.1"
                     /translation="MKTTSLPSRWVIAAVLLAAGLILTPGNRAAAPRRGCPLVLPMTA
                     FAAETAKTAETNTTARPRLMALGAGKCVSCKAMAPILKELKAEYAGRMDVEFIDVWKN
                     PDAAKAHSIKLIPTQIFFDAAGKERFRHMGFFSKKDILAKWKELGVEFTPAAKPGAKK
                     "
     gene            3222..3920
                     /locus_tag="PKI07_12415"
     CDS             3222..3920
                     /locus_tag="PKI07_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOC51888.1"
                     /translation="MESLFASLSRAVEGAPAVALGASAVWGILSIILSPCHLASLPLI
                     VGFISGQGRVSTARAGGIATLFAVGILVTIAAIGVITATAGRMMGDVGRWGNYFVAAI
                     FFVVGLHLLGVVPMPWSGPGRVGMRRKGLLAAFVLGLVFGIALGPCTFAFLAPMLAMT
                     FKLAESNLLYGVALLIAFGVGHCVVIVLAGTFTELVQRFLNWNEQSKGVIIVKGVCGV
                     LVLLGGAWLIYRAG"
     gene            4003..4929
                     /locus_tag="PKI07_12420"
     CDS             4003..4929
                     /locus_tag="PKI07_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOC51889.1"
                     /translation="MTPTRQGSIRLFRFAGIDVYLHWLWFLAAYYLIKVYAPAYTSLI
                     WPVLECLALFGIVLLHEFGHALACRQVGGQANLIVLWPLGGVAYVAPPQRPGATPWSL
                     AAGPLVNVALLPLLGAAWLLVRSAGGAQVMPDAYHFIRTVFLINTVLLVFNLLPVYPL
                     DGGQILQSLLWFIFGRARSLMIVTVIGFAGVAVLLGLALLARSLWFGILSVFILIQCW
                     NGLLRARALAKVDQLPRRAGLACPSCRQPPIIGPVWRCENCRQPFDPFAAPSVCPHCG
                     TRASACRCPECGVLQPLANWVVAPPPLAPAGY"
     gene            4938..7361
                     /gene="ligA"
                     /locus_tag="PKI07_12425"
     CDS             4938..7361
                     /gene="ligA"
                     /locus_tag="PKI07_12425"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOC51890.1"
                     /translation="MNFREAQARHARLVEEIRRHDYAYYVLAQPAISDFEYDQLYQEL
                     VALEQAHPALLTPDSPSQRVGGQPLDGFTRVRHEVPMLSLEKIKAAREPDETQEPAAE
                     KRQRQQDQNTLAELRSFDATLRKQLGRERIEYVVEPKVDGVSISVHYRNGELALGATR
                     GDGVTGDDITANLKTIRGVPLRLRLPRPPALLEVRGEAYIAKPDFAQLNARLDAAGER
                     TFPNARNATAGTLKQLDPRLVARRPLRAVFYAVGALEGISFATHAEVLTALKEFGLPT
                     PALWWVCPGMDAVLECYQRQIVCGYDETRDLRSRLPYDIDGLVLKVNNLADWPRLPAK
                     ARAPGYAIVHKPIPWITPAETVLRAITVQVGRTGVLTPVAELEPVFVQGSTISRATLH
                     NEDEIARKDIRIGDTVIIRKAGMVIPEVVEVVKAKRPAGAKPFDFIGHLGGRCPACGG
                     PITRENVAAGGQKEAAWRCQNVAGCPAQQMRRLEYFAQRKALDLEALGGIVAEKLIET
                     GLVKEPLDLFELSLEKLAALNLGTETEPRVFGEKNAGKLLEAVARARTLPLARWLLAM
                     AIPEIGEETAYDLAGYFPTLDALVASPRLADVVRLGELRRQFQENSIPKNQRHALTEV
                     EITARKQAQQEAKAQADPIGKRLLEAGFAKPSAGPAAKDWQVISRVGPVAAKAVLDWA
                     NSPAGRQVLQRMRALKLAPAGGAPGTPAAPGAEPQPLAGKRFVLTGTLSAMPRDEAAR
                     QIRSRGGDVGGSVTKNTSFLIVGAEPGAAKTGQAKKLGVPLLSEKDFLALLGDPNPPK
                     PKPAQHELF"
     gene            complement(7522..8061)
                     /locus_tag="PKI07_12430"
     CDS             complement(7522..8061)
                     /locus_tag="PKI07_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51891.1"
                     /translation="MKPYWMALFLIPFMSLTGALRAADPGKTTAPGSDDLSRYLAIKT
                     RPGESAVVRDASGRTLATARSSGNNSVVFRDAAGRTLGTATTWGGRTVFRDAAGRTAW
                     TANPSANGQNITYHDAAGRTQRTASTSGGSVTFRDAAGRTTSTLRQNNSSITARDSSG
                     RTQSTSWTSGGKPAAPAKR"
BASE COUNT         1328 a         2483 c         3025 g         1520 t
ORIGIN      
        1 aaatccaggg taagtccaag gtgcgtcgaa gccggtatta ctcaggtttt gcctctattt
       61 tatccctcca tttgcgtaaa ccattgattt tacgggcgaa aggctcggga ttcggcagcc
      121 atgcccttga atccgctaaa aaaggggctc gtcggggctg cggcctgggc ggccaggtcc
      181 gtcccggggc cccggtggaa cgagggcggg tggatgccat gcccggctga atcgttccgg
      241 tttagcactt caaccggggg ggctcccaaa ggagatgctc ctccttctaa cggtttcgcc
      301 tcgcacgccc aaagacgtct ttcctctttg cgtgccgttc tgcgtccccg ccgctttcag
      361 ccgcaggaaa caatgacgcc ccttctttgc cggagaaggg ggggctcacc ccggacggcg
      421 tcccggctct gctcgtgccg gccggggtgc ttgacgccgg ggggcggttc gcatatattt
      481 cgtttattgg cgaattgaga aagtagatga aaggcttcct gaatatcacg aaggcgctgg
      541 cggacgagaa ccggcttcgg ctgatgatgg cgctccaggg gggcgaggtg tgcgtgtgcc
      601 aactcaccga actgatgggg ctggcgatgt ccacggtgtc caaacacctt tccgtgctct
      661 accaggcggg gctggtgaag gcgcggaagg aggggcgctg gatgtattat tcgttgcccg
      721 gcaagggagc gccagcggcg gcgcgggggg cggtggtgtg ggcgcggcga tcgctggccg
      781 ggaaggagca ggtggccgcg gatgcccggc ggctcaagca ggtgctggcg atggatatca
      841 acgcgctctg ccggcggacg cgcggctccc gaagctgagc gcccggtggc cggcgccgaa
      901 atgttgccgt gaatgaatag catcacgttc caggcagggg cggggatact gaaccgcgag
      961 ttgaaagtgt ttggcgggtt cctcgccgtg ttcggattgg tgttttacct gccggtgggg
     1021 gtggcgcggt ttgacaacgc actgcaggag gggctggcgc tgacgaaggg gtatgcccgg
     1081 gagcatgtgc tgctgtgcct ggtgccggcg ttgttcatcg cggggggcat tggcacgttt
     1141 gtaagccagg cgtccgtaat gcgatacctg gggccaaaag cgaacccggc gctggcttac
     1201 ggggtggcgt cggtctcggg agcaattctg gcggtatgtt cgtgcacggt gctgccgctg
     1261 tttggcggca tctggatgcg gggggcgggg ttggggccgg cgacggcttt tctgtattcg
     1321 gggccggcga tcaatgtgct ggcggtcgtg ctgacggcac gggtgctcgg gttggaattg
     1381 ggggtggcgc gggccatcgg ggcggtgagc tttagcgtga tcgtgggact cgtaatgcac
     1441 ggggtattcc ggcgggaaga acaggccaaa gccgccgcgg cagcggcgat gcccgcggtc
     1501 gcagcccggc gcccgctttg gcagacggtc ttatttttcg gctcgatggt gggggtgctg
     1561 gtctttgcga actggagccc gccggcggaa ccggcgggac tgggggcggc gatccatgcg
     1621 tggaaatggc cgctgacggg aggttttgcg gcggggttgg gattatcgct gggattgtgg
     1681 ttcgggttca agtggcggga actgacgctg gcggcgatgc cggtggggtt gctgagtttg
     1741 gtatttcccc actcaccgct ggcggcattc atcacgggtt gcgcggcgct ggcggtgctg
     1801 tgcgcgcgga acgaaggcga ggggcgcgaa tggctggacg caacgtggac gctgggcaag
     1861 cagatatttc cgctgctgct ggccggggtg ttcctgtcgg gaatcctgct ggggcggccc
     1921 gggcacgagg ggttgatgcc gtcggagtgg gtgagccgct ccgtgggagg gaattcgctg
     1981 cgagccaact tcttctcgtc ggtggtgggc gcgttcatgt atttcgcgac gctgacggag
     2041 gtgccgattt tgcaggggtt gatcggggcc ggaatgggcc gcgggccggc gctggcgctg
     2101 ctgctggcgg ggccggcgtt gtcgctgccg agcatgttgg tgctgcggag catcctgggc
     2161 acccgaaaga caatcgtgta tgtggcgttg gtggtcgtaa tggcggccct ggccgggctt
     2221 gccggcggag tctggctcaa atgagaaggc cgcatgaaac gggcaacgca acggattaat
     2281 gggagaagct gaaaacgcaa gcacaccgat aactgacgat gaagatactc gtgattggac
     2341 ccggctgcgc aaagtgccag gcactggcgc aactgacgga gcaggcggtg aaggaactcg
     2401 gggtggccgc cgagattaac aagatgacgg acctgaagca gatcatggcg ttaggggtaa
     2461 tgatgacgcc ggcgctggcg gtcaacggca cgatcaaggt gtccggcaaa gtgccgcgcc
     2521 tggccgagat caaggcgatc ctgcagtcgg cggcctgagc cgatgggtgc ggtgcgcagc
     2581 gcgtgaagcg ggcagagtga aaccggaaag gaccgcgatg agcgaccaga cgaggggctt
     2641 cagcagccgc aaccggagcg gacgtttttc tgggcggagg acgtggcggt ccgcgttgtt
     2701 cccaaagatt aatccctgga cttgcgattt atgaaaacaa cctcccttcc aagccggtgg
     2761 gtgatcgcgg ctgtgttgct ggccgccggg ctgatcctga cacccggcaa ccgcgccgcg
     2821 gcgccgcggc gcggttgccc cctggttcta cccatgacgg ccttcgcggc ggaaaccgcg
     2881 aagacggcgg agacgaacac gacggcgcgc ccgcgactga tggcgctggg cgcgggcaag
     2941 tgcgtttcct gcaaggcgat ggcgccaatc ttaaaggaac tgaaagcgga gtatgcgggc
     3001 cggatggatg tcgagttcat tgatgtgtgg aagaacccgg acgcggccaa ggcgcattcg
     3061 atcaagctga tcccgaccca aatcttcttt gacgcggccg gcaaggaacg gttccgccac
     3121 atggggttct tttcgaagaa agacatcctg gccaaatgga aagaactggg ggtggagttc
     3181 acgcccgcgg caaagccggg cgcgaagaag taaggggccg catggagagc ctgtttgcaa
     3241 gcttgtcgcg cgcggtggaa ggcgcgccgg cggtcgcgct gggggcttca gccgtttggg
     3301 gcattttaag catcattcta agcccctgcc acctggcgag cctgccgctg attgtcgggt
     3361 tcatcagcgg gcaggggcgg gtaagcacgg cacgggcggg cggaattgcg acgctgttcg
     3421 cggtgggcat tttagtgacg atcgcggcga tcggggtgat caccgccacg gcgggccgga
     3481 tgatgggaga cgtggggcgg tgggggaact attttgtggc ggcgattttc ttcgtggtgg
     3541 gattgcacct gctgggggtg gtgccgatgc cgtggtcggg gccgggccgg gtggggatgc
     3601 ggcggaaagg actgctggcg gccttcgtcc tcgggttggt gttcggcatt gcattggggc
     3661 cctgcacgtt cgccttcctg gccccgatgc tggcaatgac gtttaaactg gctgagagca
     3721 atctcctcta cggcgtggcg ctgctgatcg cttttggcgt cggccactgc gtggttattg
     3781 tcctggccgg cacgttcacc gagctggttc agcggttcct gaactggaac gagcaatcga
     3841 agggcgtcat aatcgtgaaa ggtgtctgtg gcgtgctggt gctgctggga ggcgcctggc
     3901 tgatttaccg cgcggggtag gcgcgcgcgt tgcgcgggcg gcgtcctccc ggccctgaaa
     3961 aggcggattg ccgtgcccgc tggggtgtgt taggtctgcc ttatgacgcc gacacggcaa
     4021 gggtccatcc gcctgttccg ctttgcgggc attgatgtgt atttgcactg gctgtggttt
     4081 ttggccgcgt attatctgat caaggtctat gcgccggctt acacttccct gatctggccc
     4141 gtgctggaat gcctggcgct gtttggcatt gtgctgctgc atgagtttgg ccacgcgctg
     4201 gcctgccggc aggtgggcgg gcaggccaat ctgatcgtgc tttggccgct gggcggggtg
     4261 gcgtatgtgg cgccgccgca acgcccgggc gcgacgccct ggagcctcgc cgccgggccg
     4321 ctggtgaatg tcgcgctgct cccgctgctg ggcgcggcct ggttgctggt ccggagcgcc
     4381 ggcggagcgc aggtcatgcc cgatgcttac catttcatcc gaaccgtctt cctcatcaac
     4441 accgtgttgc tggtcttcaa cctgttgccg gtttacccgc tggatggcgg gcagattctc
     4501 caatccctgc tctggtttat tttcgggcgg gcgcgcagcc tgatgatcgt cacggtgatt
     4561 ggctttgccg gggtggcggt gctgctgggg ctggctttgc tggcgcgcag cctgtggttc
     4621 ggcatcctga gcgtgttcat cctcatccaa tgctggaacg ggctgctgcg ggcgcgagcg
     4681 ttggcgaaag tggaccaact gccgcgccgc gccgggctgg cctgcccgtc gtgccgccag
     4741 ccccccatca tcggcccggt ctggcgctgt gaaaactgcc ggcaaccctt tgacccgttt
     4801 gcggctccgt ccgtttgccc gcactgcggg acgcgcgcca gcgcctgccg ctgccccgag
     4861 tgtggcgttt tgcagccgct ggcgaactgg gtggtggcac cgccgccgct ggcgccggcc
     4921 ggttattgag tcccgccatg aacttccgcg aagcccaagc ccgccacgcc cggctggtcg
     4981 aggaaattcg ccggcatgat tacgcctact acgtgctggc gcagcccgcc attagcgatt
     5041 tcgaatacga tcagctttac caggagctgg tggcgctgga gcaggcgcac cccgcgctgc
     5101 tcaccccgga ctcgcccagc cagcgggtcg gcggccagcc gctcgacggc tttacccgcg
     5161 tccggcacga ggtgcccatg ctttcgctgg agaaaatcaa agccgcccgg gagccggatg
     5221 aaacgcagga gccggcggcc gaaaagcgcc agcggcagca ggaccagaat accctggcgg
     5281 agctgaggag ctttgatgcc accctccgga agcaactggg ccgggagcgc atcgagtatg
     5341 tggtcgagcc caaagtggat ggcgtctcga tctccgtgca ttaccggaac ggcgagctgg
     5401 cgctcggggc cacccggggc gacggcgtca cgggcgacga catcaccgcc aacctcaaga
     5461 ccatccgggg cgtgccgctg cggctgcggc tcccgcgccc gcccgcgctc ctggaggtgc
     5521 gcggcgaggc ctacatcgca aagccggact tcgcgcaact caacgcccgg ctcgacgccg
     5581 ccggcgagcg aacctttccc aacgcgcgca atgccaccgc ggggaccttg aagcaactgg
     5641 acccgcgcct ggtcgcccgg cgcccgctcc gggccgtctt ttatgcggtg ggcgccctcg
     5701 agggcatctc gtttgcgacg cacgcggagg tcctcaccgc cttgaaagag tttggcctgc
     5761 ccacgcccgc gctttggtgg gtctgcccgg ggatggacgc ggtgctggag tgctatcagc
     5821 ggcagatcgt atgcggctat gacgagacgc gcgatctgcg ctcccggctg ccgtatgaca
     5881 ttgacgggct ggtgctcaag gtcaacaacc tggccgattg gccgcgcctg cccgccaagg
     5941 ccagggcccc cggttatgcc atcgtgcata agcccattcc ctggatcacg ccggcggaaa
     6001 ccgtgctgcg ggccatcacc gtgcaggtcg gccggacggg cgtgctgacg cccgtggcgg
     6061 agctcgaacc ggtgttcgtt caaggctcca ccatttcccg ggccaccctg cataacgaag
     6121 acgagattgc ccggaaggat attcggattg gggacaccgt cattatccgc aaagccggca
     6181 tggtcatccc ggaggtggtg gaggtggtca aggcgaagcg gccggccggg gcaaagccct
     6241 ttgactttat cgggcatctc gggggccggt gccccgcgtg cggcggcccc atcacccggg
     6301 aaaacgtggc cgcgggcggc cagaaggaag ccgcctggcg ctgccagaac gtggcgggct
     6361 gcccggcgca gcagatgcgg cggctcgaat acttcgcgca gcggaaggcg cttgacctcg
     6421 aagccctggg gggcatcgtc gccgagaaac tgatcgaaac ggggctggtg aaagaaccgc
     6481 tggacctgtt cgagctttcc ctcgaaaaac tggccgccct caacctggga accgagaccg
     6541 agccgcgcgt gtttggcgag aagaacgcgg gcaagctcct ggaggccgtc gcgcgcgcgc
     6601 gcaccctgcc gcttgcgcgc tggctgctgg ccatggccat tcccgagatt ggcgaggaga
     6661 ccgcttatga cctggccggg tatttcccca ccctggacgc gctggtggcc tcgccccggc
     6721 tggcggatgt tgtccgcctt ggcgaattgc gccggcagtt ccaggaaaac agcatcccca
     6781 aaaaccagcg gcacgccctg accgaagtgg aaataaccgc gcggaagcaa gcccagcagg
     6841 aggccaaggc gcaggcagac ccgatcggaa aacggctgct ggaggcgggg tttgccaaac
     6901 cctccgccgg gccggccgcc aaggactggc aggtcataag ccgggtgggg ccggtagcgg
     6961 ccaaagccgt tttggattgg gccaacagcc cggccgggcg gcaggtcttg cagcgcatgc
     7021 gcgcgcttaa actcgcgccc gccggcggcg cgcccgggac gcccgcggcg cccggcgcgg
     7081 agccgcagcc cttggcgggg aaaaggtttg tcctgaccgg cacgctatcc gcgatgccgc
     7141 gggatgaagc agcccggcag atccgatccc ggggaggcga tgtgggcggt tcggtgacga
     7201 aaaacaccag cttcctgatc gtcggcgcgg aacccggagc cgccaaaacc gggcaagcaa
     7261 agaagctggg agtgcccctc ctgagcgaaa aagacttcct ggccctgctg ggcgacccga
     7321 accccccgaa acccaaaccc gcccagcacg aactctttta gcccgcccgt tgccggccgg
     7381 gcgccgcgcg cgagccgggc atggctggga atgggccggt gctccccgcc ttgtggccaa
     7441 gctgccgggg gatgaaaatg cccgcgaatg agggattatt ggatccggcc cggaatgggg
     7501 cggttccaat ttccccaacg gctatcgctt tgcgggggcg gccggttttc ctccggaggt
     7561 ccaactggtg ctttgggtcc ggccgctgga atcgcgcgcg gttatgctcg agttgttttg
     7621 ccgcagggtg ctggtcgtgc gcccggcggc atcgcggaac gtgacgctgc cgccggaagt
     7681 tgaagccgtc cgctgcgtgc gcccggcggc atcgtggtag gtaatgtttt gcccgttggc
     7741 ggaggggttt gcggtccagg cggtgcggcc cgcggcatcg cgaaaaaccg tccgcccgcc
     7801 ccaggtggtc gcggtgccca gggtgcggcc ggcggcgtcg cggaagacga cggagttgtt
     7861 gccgctcgac cgagccgtgg ccaaagtgcg gccgctcgcg tcccgcacaa cggcgctttc
     7921 tccgggtcgg gtcttgatgg ccaggtagcg gctcaggtca tccgagcccg gggccgtggt
     7981 tttcccggga tcggcggcgc gcagcgcgcc ggtgagggac atgaagggga tcaagaacag
     8041 cgccatccag tatggtttca taacgcactc agcattttcc aacccgcggt tcaggttaat
     8101 tattcgggaa ggcgagcggt ttggcaagcc ttgattcctc ccgcgacttc gcaagtttca
     8161 ggcccgcgca aaccctcgcc tccccccgcg tgaatcctct aacacgctca atagacggac
     8221 gaaaccgggc cggcgtttat tcagccgccg cgcctgggcg gcccgccgga cgggccggcc
     8281 gttgacgccc cggcgggccg tgctaaccgg gatatttcac gccccgcgcg aaatacccgc
     8341 aggccggagc gcgggc
//
LOCUS       DAOPQG010000136         8355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_9524_length_8355_cov_13.634819, whole genome shotgun sequence.
ACCESSION   DAOPQG010000136 DAOPQG010000000
VERSION     DAOPQG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9524_length_8355_cov_13.634819"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..656)
                     /locus_tag="PKI07_12435"
     CDS             complement(369..656)
                     /locus_tag="PKI07_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51892.1"
                     /translation="MGSGFWSESVYQFKAADRREIAEIARLATTSTQRQCHRLELPRK
                     KGTPSFYKVPGYAVFCQMLTRRNPKAFAARQDTPSEALNRRPIRSLSLPGS"
     gene            797..1984
                     /locus_tag="PKI07_12440"
     CDS             797..1984
                     /locus_tag="PKI07_12440"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOC51893.1"
                     /translation="MRHLILALALLLVMLIGAPSLTRARPAAGSGAPGIHKLKDLVIY
                     HDDRFYSSFPSVIRRPNGELLVAFRRAPERRTLGERSTSHTDPNSYLVLVRSRDNGQT
                     WSPTPELIYAHPLGGSQDPCMVQLRDGTILCASYGWAWLQTEAADKLIKPLRHGNFVF
                     LGGYLLRSRDNGRSWQGPIIPPPVPGETVLDPFNQPVPAYNRGALCQGKDGRLYWAVT
                     SKTSSPPGAQGTHLLLSADQGSTWAYAGPIATDAKVVFNETALYETPKGDLVAFMRTA
                     NFDDHTAVARSTDRGRSFEPWQDAGFQGHPHHALRLPDKRVLLVYGYRHAPFGIRARV
                     LDPECRNLAAAKEIILRDDGGSGDLGYPWATLISKRRALVVYYFNTANGTRHIAGTLL
                     ALE"
     gene            complement(2050..3183)
                     /locus_tag="PKI07_12445"
     CDS             complement(2050..3183)
                     /locus_tag="PKI07_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51894.1"
                     /translation="MIIRRLSLFVLALGLGARALAQTMPERLRAELDQLPHPRSVADF
                     QAAPCLSHVNQNKTSFCWSFATCSFLEAEMARRQLPPVRLSPMYAAYCQYVEKARRFV
                     QTRGESRVSPGDTFWGVFDACAQYGAMPSAVYEKLPDNPPFDHTRLYAELGRCLQEVK
                     LRGQWNEARVLDRIRKILNRHLGKPPETFAHNGKTYTPRSFLAEVVRLPWSEYISVVS
                     QESAPFNAFTELKVPDNWRHHTNFFNVPLPVFYEAFRGALRGGFTVAVEIDTSEPSYE
                     TLVRHCFIPSFDLPARELNQAARETRLRNGATGDDHAIHIIGWSEPGGEDWFLAKDSG
                     KEAWRDGNQGIVFLHGAYVKLKVLAFIVHRDGVPPVKALLPPR"
     gene            complement(3203..4468)
                     /gene="larC"
                     /locus_tag="PKI07_12450"
     CDS             complement(3203..4468)
                     /gene="larC"
                     /locus_tag="PKI07_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOC51895.1"
                     /translation="MKTLYLDLFSGISGDMFIAALIDLGVEADWLEQELQQLRLGGYR
                     LRVTRARRASLAGVRFEVLLDALHRHGEPAPAPAHPHSHEHGRTFAQIRQLIAGSRLS
                     EGVKQKAVAIFQRLAVAEGKIHGCPPEQVHFHEVGAVDSILDIVGACLALERLGQPRV
                     LAGPVVEGTGWIECAHGRFPIPAPATLAILGARGIPLSQCDEPHELVTPTGAALLAEL
                     VESFGPMRELVAEKTGYGLGSREHRSRPNALRAVLGETRSVGRASETLGTHDWETDSI
                     VALETNLDDINAEILGHFMEQALAAGARDVFHTAIQMKKNRPAVLLTVLCDEADADRF
                     TELLLRETSAFGVRRHAVERRKLRREWMTVPTPHGDASVKVGRLGGKVLQAAPEFESC
                     RRLAAQAGVPLKAVYEAVLRALPPSLKKG"
     gene            complement(4470..5174)
                     /gene="larB"
                     /locus_tag="PKI07_12455"
     CDS             complement(4470..5174)
                     /gene="larB"
                     /locus_tag="PKI07_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOC51896.1"
                     /translation="MGRDEVLRQFQVAPLVDLGFATVDTHRALRKGFPEVIFGAGKTP
                     AQVVAIAAKLFAHEPRVLVTRVGAETARKLRRRFRAAVYHELARCVTIEKPPLPKRPG
                     VIAVVCAGTSDLPVAEEAAVTAAIMGNRVERITDVGVAGVHRLFGRLEAIQRANVVIV
                     VAGMEGALPSVVAGLVSRPVIAVPTSVGYGASFQGLAALLAMLNSCASGLTVVNIDNG
                     FGAGYAASQINALAAG"
     gene            complement(5251..6879)
                     /locus_tag="PKI07_12460"
     CDS             complement(5251..6879)
                     /locus_tag="PKI07_12460"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOC51897.1"
                     /translation="MAEPKTGELMEKIVSLCKRRGLIFQSSEIYGGLNGFWDYGPLGA
                     ELKRNIKEHWWRSMTQLRDDVVGLDASIIMHPKIWEASGHTSTFSDLMVDCLLTKKRF
                     RADQIEPQSGMAYFYTGATDSGSGRASKDAFSVLIATGKHADYARKVARQFYQQRGLE
                     NPSLEGERTEAQANTTRYNPENGSLLTEPRPFNLMLKTYVGPVESADNISYLRPETAQ
                     AIFVQFKSALEVSRQRVPFGICQVGKAFRNEINPRNFTFRSREFEQMELEFFIRPDEV
                     VQKMCGTVAGLSDCPPGALVPQPNWGWELWHKYWVEQRIQWYESIGLGRDTLVEYWQK
                     KEELAHYAKATVDILYKFPFGTQELEGVAARGDFDLTQHQKFSGKSMEYFDEEAKAKY
                     VPHVIEPSAGVDRLALALICNAYCEDQAPDEKGNLETRVVMRFHPRIAPIKVAVFPLL
                     KNKPELVKKAREVRELLRPHMTVFYDEAGAIGRRYRRQDEAGTPFGVTIDFDTLGEQN
                     AARQDTVTLRDRDTMKQERVKIADLVPLLREKIQ"
     gene            7314..>8355
                     /locus_tag="PKI07_12465"
     CDS             7314..>8355
                     /locus_tag="PKI07_12465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOC51898.1"
                     /translation="MNTKQLLTLSTVSLAGASAALATADYGPAVDRMITGCTKWYTSG
                     YGHKFAVVHSMEGYYLTGTSYLRRCDISASCQYTVNGLVDYAGDAPRGEISQLVREAY
                     YAWHATCWNQHSLGTEHEGFVSNPAWFTEEMYQASALLHRHFADKFGFAKDRNHIVGH
                     GEKSNGAWCAYASANLGINPYCNTHTDPGPYWNWSHYMALIVGSVDGATVVSISAPSS
                     VNVGQSFTATVVMRNSGSTTWTTANGYKLGSQSPQDNSTWGFGRVALPGTISPGANAT
                     FTFTATAPGTVGSYAFAWKMVREGVQWFGGTASTTIAVVNPLPTITTQPVSVTKNPGE
                     TATFTVGATGATS"
BASE COUNT         1504 a         2730 c         2515 g         1606 t
ORIGIN      
        1 gggcgggtgg ggcgggtttg ggcgcgggcc tgagccggat tggagggatg gacgagtcgc
       61 ggatgatgta aaacgacgtt ccaaagcagg agggagagaa cgcgaacgtt cggcaaagcc
      121 gggacgggcg gcgtggtgtt gtcgcgcgcc ttggtttttg ggcggtctgt ggctacggct
      181 ggcgaggaca tttcccctcc cgggcggggg ttgccgaaag gcggctgctt gcgttgtcct
      241 catcgtgggc ggcgtattcg tccggttggg atgagtttgg ttgacgattt atccccaggc
      301 tttgcgcgca tggtgagtga tgaacgggat tggttcggga acctgttcag gggctttggt
      361 tttgttgttc aggagccggg gaggctgagg ctgcggattg ggcggcggtt aagcgcttcg
      421 gagggggtgt cttgccgcgc ggcaaaggct ttggggttcc tgcgcgtgag catctggcaa
      481 aacacggcat agcccggcac cttgtaaaag cttggagttc cctttttccg cggcaattcc
      541 aaacggtgac actggcgttg cgtcgaggtg gtggcaaggc gggcgatttc ggcaatctcg
      601 cgccggtcgg ccgctttgaa ttgatagacg ctttcactcc agaaccctga acccataaag
      661 cggctttggg tttagcaaaa gtttttctcc ctcatctgca aaaactcagg cgctggcaac
      721 caaaggccct ggatgctgac gccctccacg ggcgtttcta cttgcgcgcg cggcggattc
      781 cgtccatgca agggtcatgc gtcacctgat tcttgccctg gctctgctgc tcgtcatgct
      841 catcggcgcc ccgtccctca cccgcgcccg gcctgcggcc ggctccggcg cccccggcat
      901 ccataagctc aaagaccttg tcatctatca tgatgaccgg ttctacagca gctttccctc
      961 cgttatccgc cgcccgaatg gcgaactgct tgtggcattt cgccgtgcgc cggaacgccg
     1021 caccctgggc gagcgcagca ccagtcacac cgacccgaac agctacctgg tgctggtgcg
     1081 ctcgcgtgat aatggccaga cctggagccc gacgcccgag ttgatctacg cccacccttt
     1141 gggcggctcg caggacccct gcatggtcca actgcgcgac ggcaccatcc tctgcgccag
     1201 ctatgggtgg gcgtggctgc aaacggaggc cgcggacaaa ctgataaaac ccctccgcca
     1261 cggcaacttc gtcttcctgg gcggctacct gttgcgctcg cgcgataacg ggcgctcgtg
     1321 gcaggggccg atcattccgc cgcccgttcc gggcgagacg gtgcttgacc cgttcaacca
     1381 gcccgtgccc gcctacaacc gcggcgccct gtgccaaggc aaagacggcc ggctctactg
     1441 ggcggtcacc tccaaaactt cctccccgcc gggggcgcag ggaacgcacc tgctcctttc
     1501 cgcagaccaa ggcagcacct gggcttatgc cggccccatc gcgacggatg ccaaagtggt
     1561 ctttaatgaa accgcgctct atgagacgcc caaaggcgat ctggtcgctt tcatgcgcac
     1621 cgccaacttt gatgaccata ccgccgtggc gcgctcgacc gaccgcgggc gcagtttcga
     1681 gccgtggcag gatgccggct ttcaaggcca tcctcatcac gcgctgcgcc ttccggacaa
     1741 gcgcgtgctg ctggtctatg gctaccgcca cgcgccgttc ggcatccggg cccgcgtgct
     1801 ggacccggaa tgccggaacc tggccgcagc aaaggaaatc atcctgcgcg atgacggtgg
     1861 cagcggcgac cttggctatc cctgggcgac gctgatctcg aagcggcgcg ccctggtcgt
     1921 ttactacttc aacaccgcaa acggcacccg ccacattgcc ggcactctcc tggccctgga
     1981 atagccccgg ggactgcccc gccctgcccc accgcggcgc cccggcccgg cccccctcag
     2041 gcccgccggt tagcgcggcg gcaggagagc cttgaccggc gggactccat cgcggtgcac
     2101 aatgaaggcc aggaccttca gcttcacgta tgcgccgtgc aggaagacaa tgccctggtt
     2161 gccgtcgcgc caggcttcct tgcccgagtc cttggcgagg aaccaatcct cgccgcccgg
     2221 ctcactccag ccgatgatat ggatggcgtg atcgtcgccg gtcgccccat tccggaggcg
     2281 cgtctcgcgc gcggcctggt taagttcgcg ggccgggaga tcgaagctgg gaataaaaca
     2341 atggcgcacc agggtttcat aactgggctc gctggtgtct atttccaccg ccacggtaaa
     2401 gccgccgcgc agcgcgccgc ggaaggcttc gtaaaatacg ggcagcggca cgttaaagaa
     2461 gtttgtgtgg tgccgccagt tgtccggcac cttcaactcg gtgaacgcgt tgaacggcgc
     2521 ggattcctgg gaaaccacgc tgatatattc ggaccagggc agccgcacaa cctccgccag
     2581 gaaggaccgg ggcgtgtagg ttttcccatt atgcgcaaaa gtctccggcg gtttgcccag
     2641 atgccggttc agaatcttcc gaatccggtc caggacgcgc gcttcattcc attggccgcg
     2701 cagtttgacc tcttgcaggc agcgccccag ttcggcatag agccgggtgt gatcgaaggg
     2761 cggattgtcc ggcaacttct cataaacggc ggaaggcatg gccccgtatt gggcgcaggc
     2821 gtcgaacacg ccccagaacg tgtcgcccgg cgagacgcgc gactcgcccc gggtttgcac
     2881 aaaccggcgc gctttctcga cgtattggca atacgcggca tacatgggcg aaagccgcac
     2941 cggcggaagc tgacggcggg ccatttccgc ttccaggaag gagcacgtcg caaagctcca
     3001 gcaaaacgac gttttattct ggttaacgtg cgacaggcag ggggcggctt gaaagtccgc
     3061 gacgctccgg ggatgcggca actggtccag ttccgcccgc agccgctccg gcatagtctg
     3121 ggccaacgcc cgggccccca gccccagggc caaaacgaac aagctcaatc gtcggatgat
     3181 catagcactt cggcgcatca ttctatcctt tcttcagtga gggcggcaag gcgcgaagaa
     3241 cggcttcata gaccgccttc aacggcaccc cggcctgcgc ggcgagccgg cggcacgact
     3301 caaattccgg cgcggcctgg agcactttgc ccccgagccg tcccaccttc acggatgcat
     3361 cgccgtgcgg ggttggcacc gtcatccatt cgcgccgcag cttgcgccgt tcgacggcat
     3421 gacggcggac gccaaacgcg ctggtctcgc gcagcagcag ctcggtgaac cggtccgcgt
     3481 cggcctcgtc gcacagcacc gtcaggagca cggcgggccg gttcttcttc atctggatgg
     3541 ccgtatggaa aacgtcccgg gcgccggcgg cgagcgcctg ctccatgaag tggcccagaa
     3601 tctcggcgtt gatgtcgtcc aggttcgttt cgagcgccac aatcgagtcc gtctcccaat
     3661 cgtgggttcc gagggtctcc gatgcccggc ccaccgaccg ggtttccccg agcaccgccc
     3721 gcagcgcgtt ggggcgcgac cgatgttcgc ggctgcccag gccatagccc gtcttttcgg
     3781 ccaccagttc ccgcatcggg ccgaaggatt caaccagctc cgccagcaat gccgcgcccg
     3841 tgggcgtgac cagttcgtgg ggctcgtcgc actgagaaag cggtatcccg cgcgcgccca
     3901 ggatcgccag cgtggcgggg gcgggaatcg gaaaccgccc gtgcgcgcac tcgatccagc
     3961 ccgtcccctc caccaccggc cccgccagga cgcgcggctg ccccagccgc tccagcgcga
     4021 ggcacgcccc gacgatgtcc agaatcgaat ccaccgcgcc cacttcgtgg aaatgcacct
     4081 gttcgggcgg gcagccgtga atctttcctt ccgccacggc gagacgttga aagattgcca
     4141 ccgccttctg tttgactccc tcggagagcc gacttccggc tatcaattgc cggatttgcg
     4201 caaaggttcg cccgtgttca tgcgaatggg ggtgcgccgg cgcaggcgct ggttcgccgt
     4261 ggcggtggag ggcatcaagc agcacttcaa atctgacccc ggcgaggctg gcccgccgcg
     4321 cgcgcgtcac gcgcaggcgg tagccgccca ggcgcagttg ctgcagctcc tgctccagcc
     4381 agtcagcctc aactcccagg tcaatcaacg cggcaataaa catgtcgccg ctgatgccgc
     4441 tgaataaatc gagataaagc gtcttcatgt taaccggccg ccaaagcgtt gatttggctg
     4501 gccgcatagc cggcgccgaa accgttgtca atgttcacta ccgtcagccc gctggcgcaa
     4561 ctgttaagca tggccagcaa cgccgccagg ccctggaagc tcgcgccgta gcccacgctg
     4621 gtcggcaccg cgatgaccgg ccgggacacc agtccggcca ccacgctggg caaggcccct
     4681 tccatgccgg ccaccacaat gaccacattc gcgcgctgaa tggcctccaa ccggccaaac
     4741 agccgatgca cccccgccac gcccacatcc gtgatccgct ccacgcggtt gcccatgatc
     4801 gcggcggtga cggcggcctc ttcggccacc ggcaggtcgc tggtgccggc gcaaaccacg
     4861 gcaatcacgc ccggccgctt cggcaacggc ggcttctcga tcgtcacgca acgcgccaac
     4921 tcgtgataga ccgccgcccg gaaccgccgg cgcagcttgc gcgcggtttc ggcccccacg
     4981 cgggtcacga gcacgcgcgg ctcatgcgca aagagtttgg ccgcaatcgc cacgacctgg
     5041 gccggcgtct tgcccgcgcc gaaaatgacc tcggggaagc ccttgcgcag cgcgcggtgc
     5101 gtgtccaccg tggcgaatcc caggtccacc agcggcgcca cctggaactg ccgcaggact
     5161 tcatcccgcc ccacccctcc cgcccgaaac ttctccagca gcttgatggc ttcggtcgtg
     5221 gtcacagctt tggcttcgcg ccgctttccc tcactggatt ttttcgcgca gcagcgggac
     5281 caggtcggcg attttcacgc gctcctgctt catggtgtcg cgatcacgca gcgtgaccgt
     5341 gtcctgccgc gcggcgttct gctcgcccag cgtgtcgaaa tcaatcgtca ccccgaacgg
     5401 cgtcccggct tcatcctgcc ggcggtagcg gcggccgatg gctccggctt catcatagaa
     5461 aaccgtcatg tgcggccgca gcagctcgcg cacttcccgg gcctttttca ccagctcggg
     5521 cttgttcttg agcagcggaa agaccgcgac cttgatcggc gcgatgcggg gatggaatcg
     5581 catcaccacc cgcgtttcga ggttgccttt ctcgtccggg gcctggtctt cgcaataagc
     5641 attgcagatc agggccagcg ccagccgatc cacccccgcc gacggctcga tcacatgcgg
     5701 cacatacttg gccttggcct cttcgtcgaa atactccatg gatttgccgc tgaacttctg
     5761 gtgctgcgtc aggtcaaaat cgccccgcgc cgcgactccc tccagctcct gggtgccaaa
     5821 ggggaacttg tacaaaatgt ccacggtcgc cttggcgtag tgcgccagct cctccttctt
     5881 ctgccagtat tcgaccagcg tgtcccgccc caggccgatg ctctcgtacc actggatgcg
     5941 ctgttccacc cagtatttat gccacaactc ccaaccccag ttgggctgcg gcacgagggc
     6001 cccgggcggg caatcggaca gtccggcgac ggtgccgcac attttctgca ccacctcgtc
     6061 cgggcggatg aagaactcca gctccatctg ctcgaactcg cgcgagcgga aggtgaaatt
     6121 gcgcggattg atttcattgc ggaaggcctt gcccacctgg cagatgccaa acggcacccg
     6181 ctggcgcgaa acctcgagcg cgctcttgaa ctgcacaaag atcgcttgcg ccgtctcggg
     6241 ccggagatac gaaatgttgt ccgcgctttc aaccgggccg acgtaagtct tgagcatcag
     6301 gttaaaaggg cgcggctcgg tcaacaggct gccgttctcc ggattatagc gggtcgtatt
     6361 ggcctgcgcc tcggtgcgct cgccctccag cgacggattt tccaggcccc gctgctgata
     6421 gaactgccgg gccaccttgc gcgcgtaatc cgcatgcttg cccgtggcaa tcagcaccga
     6481 aaaggcgtcc ttggaggccc tgccgctgcc ggagtctgtc gccccggtat aaaagtaagc
     6541 catgccgctc tgcggctcga tctgatcggc gcgaaaccgc tttttcgtga gcaggcaatc
     6601 caccatcagg tcgctaaacg tgctggtgtg gccgctggcc tcccaaatct tcgggtgcat
     6661 aatgatcgag gcgtccaacc ccaccacatc atcccgaagc tgggtcatcg agcgccacca
     6721 gtgctccttg atgttgcgct tcaattcggc gcccagcgga ccatagtccc aaaaaccatt
     6781 caatcccccg tagatttccg atgattgaaa aatcaatccc cgccgtttgc aaagcgatac
     6841 aatcttttcc atcaactcgc cggttttagg ttcagccata ggcggggaag tctctcacag
     6901 ccttcgcgta tgtcaaggtg tcgccacgct gaatggtccg ccactatagt cgaggcgtaa
     6961 ggtctgggct gtcaccccca tccagatgcc gcctaagtca tcggtcacac tttgccctca
     7021 atggctcaaa ggcacgtggt gagcatccaa atgaataggt tcggtttagg caagcccgag
     7081 gcaggtaagc cagggggcgg acctccttgc agcaccagag ctgcggaatt ggaattgctg
     7141 ggccattttc ccattcctgc gtcctggggc gaggcatttt tcctaaattt gttgttgcac
     7201 cgttctcacg ttaaagctaa tgtagcgtta gttaaaagat aaggcctatg cggtcgaatc
     7261 gaagtcgttt caaactatgt ccgataagat atatcggtaa attttagcca cgtatgaaca
     7321 ccaagcaact cctcaccctt agcaccgttt cgctcgccgg cgcatcagcc gccctggcta
     7381 ccgccgatta tgggccggcg gtggatcgca tgatcaccgg gtgcaccaaa tggtacacct
     7441 ccggctacgg ccacaagttt gccgtcgtcc atagcatgga gggatattac ctgaccggca
     7501 cctcctattt gcggcgctgc gatatttcgg ccagttgcca gtatacggtg aacgggttgg
     7561 tggattatgc cggggatgcg ccgcggggcg agatttcgca attggtgcgg gaggcatatt
     7621 atgcctggca tgcgacgtgt tggaatcaac attccctggg cacggagcac gagggatttg
     7681 tgagcaaccc ggcctggttc accgaggaga tgtaccaggc ctcggccttg ttgcatcggc
     7741 attttgcgga caagtttggc tttgccaagg accggaatca tattgtgggg cacggggaaa
     7801 aatcgaacgg ggcgtggtgc gcgtatgctt cggcgaacct gggcatcaat ccgtattgca
     7861 acacccatac cgacccgggt ccctattgga actggtcgca ttacatggcc ttgatcgtgg
     7921 gctccgttga tggagcaacg gtggtcagca tcagcgcccc ctccagcgtg aatgtcggcc
     7981 agagtttcac cgcgactgtg gtcatgcgta atagcggttc cactacctgg accaccgcca
     8041 atggatacaa actcggctca caaagccctc aggataattc cacttgggga ttcggacggg
     8101 ttgctttgcc cggcacaatc agtcctggcg ctaatgccac cttcaccttc accgccacgg
     8161 ctccaggcac ggtcggcagc tatgcttttg cctggaagat ggtcagggaa ggtgtgcagt
     8221 ggtttggcgg caccgcttcg acaaccattg ctgtcgtcaa cccgctgcca accattacca
     8281 cgcaaccggt atcggtgact aagaatcccg gggagacggc gacctttacg gtgggggcga
     8341 cgggggcgac cagtt
//
LOCUS       DAOPQG010000137         8344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_9538_length_8344_cov_13.374593, whole genome shotgun sequence.
ACCESSION   DAOPQG010000137 DAOPQG010000000
VERSION     DAOPQG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9538_length_8344_cov_13.374593"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..779
                     /locus_tag="PKI07_12470"
     CDS             417..779
                     /locus_tag="PKI07_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51899.1"
                     /translation="MKMMHWNSVIPAASWVLAAGLLLLAGCEQKPKGPQPTVINGVEV
                     DLAKFQQAFLNAAPEVQTSVSRVHLAVRYGQYAQAEAMARKIVHLPGLTEAQQAVAQE
                     VHRQLQELVRRTPTRPPP"
     gene            911..1720
                     /locus_tag="PKI07_12475"
     CDS             911..1720
                     /locus_tag="PKI07_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOC51900.1"
                     /translation="MVLPADYHMHTPLCRHATGEPWEYAAQARQLGLPEIGFADHGPM
                     PRDDFDDWRMKAGELGEYVARVERARRDHPQLVIKLGLEVDYLPGCEDWIRELAGRHP
                     WDYFIGSVHYLSESWAIDNPAQLARWKERAPLEVWTAYFERLTQAAESGLFDILGHVD
                     LCKKFAIYPPEDCTPLYHRFLQAARRHGVAIEINTAGLRKDCRELYPGFHLLQLAAQA
                     GVPLTFGSDAHAPNEVGLNFAEAVAQARRAGYTQCCRFTRRQRQVAPLPLS"
     gene            complement(1713..2645)
                     /gene="rbsK"
                     /locus_tag="PKI07_12480"
     CDS             complement(1713..2645)
                     /gene="rbsK"
                     /locus_tag="PKI07_12480"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOC51901.1"
                     /translation="MTKPSILVVGSSNTDMIIKVQRIPQPGETILGGEFALAAGGKGA
                     NQAVGAARAGGAVTFIARLGRDMFGDQALAGFVADGINVKHVVRDPAAPSGVALIFVA
                     RNGENSIAVASGANARLAPADLQRAQAAFRRAAVVVLQLETPLPTVTAAVKLAARAGA
                     RVILNPAPARPLPASLLRHVYLLTPNESEAQLLTGMAVHSEAAAARAADALLARGVQN
                     VILTLGARGAFVAGPQARGRVPGFKVRPVDTTGAGDVFNGALAVALAEGKPLLEAARF
                     ANAAAALSVTRLGAQTSAPTRGEIDRRLAAARSN"
     gene            complement(2682..3692)
                     /locus_tag="PKI07_12485"
     CDS             complement(2682..3692)
                     /locus_tag="PKI07_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HOC51902.1"
                     /translation="MFIVESYPLAVALCIVTMLCWGSWANTQKLASKEWRFQLFYWDY
                     AVGVLLLSLLLAFTLGSSGSGGRGFLADLQQAEPKWLGSAFLGGVIFNLSNILLVAAI
                     DIAGLAVAFPVGVGLALVLGVITTYLATHQGNVHMLALGVAAIVIAIILDGLAYRRLA
                     AGGQKTPAKGIVISVAAGLLMGWFYSFVAQAMGTIDPDTHVLTPGRLSPYTAIVLFSA
                     GLLLSNFIWNSIMMVKPFSGPPVAFADYFTKGNIRLHLIGILGGMIWNLGMAFSIIAS
                     TKAGAALAYGLGQGATMIGAFWGVFIWKEFKDAPPGTNKFLAAMFAFYLLGLAILVAS
                     KL"
     gene            3960..5744
                     /locus_tag="PKI07_12490"
     CDS             3960..5744
                     /locus_tag="PKI07_12490"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOC51903.1"
                     /translation="MKLLHGLVGGVVLGAVAGLPRAEGAEPVWNIRDKPLERVSVVAH
                     RGAGDLAPENTMEALELSWSMGCIPELDVRTTKDGHLMMFHDKNFARIMPHAPPEMKK
                     KRLEDLTYAEAKQLDVGAFRGPQFAGQRIVSLAEICAALKKDRRRAAYIDVKNVDFAK
                     MARETAGVHEQIVLATGSEADCAAWKAVAPRSEAFLWVGTWGDTNDVNVEKRFARLRE
                     INFAHIDRLQVHCHFNTNNNRMVPSDDFIRRAGVEVRARGIEFQTMPWNVPQLEAAYT
                     RLLDLGTEGFGTDRPDVALRALRAYYRNGPPDWNVRDHIPRARFTVQAHRGAGDLAPE
                     GSKEAFELGWKLGCIPEADLRETRDGVIVSFHDNNFARILPNAPEELKKKGVADLTFA
                     EVKELDVGSFRNQQFAGQRVISLAEMVGILKAHPERSLYIDIKKVDFHKLAAATAEVH
                     PQLILASTKYDEIKLWREVAPKSRTLHWMGGPAAWMSGPPDAPNGPQAKLAQRLQALR
                     EVNFAGIDQLQIHVNTSPEGVFYPSEDFLREAGRELRAHGILFQTLPWKQKDAKVFHR
                     LMDLGCASFATDYPDAAQAAVSDYYRAR"
     gene            complement(5812..7851)
                     /locus_tag="PKI07_12495"
     CDS             complement(5812..7851)
                     /locus_tag="PKI07_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC51904.1"
                     /translation="MKTLLVLGAHPDVTEAIRAALNPDLYRVLDRANLEEAEPFLAHG
                     LAEACIVDFELTGVQGIWLLEKLRRRDPRCPIIIYAGAKQPEWEEEAYLQGATHILTK
                     PVRGRLLAALLERLWPKSAAARPAPLPAPPPPEAPAPIEKAAPAGAVPTLSVLRNFSG
                     ILTHSLNTEGMLQQFLLLLREILGLNRAAIFLRPPLTAFGHAPAQAESRRLHAAGSLG
                     ISRSLLHHFELSLEGGIGGHLLRLGRILRRNSEEARHDPETRNEFELLGAQVAVPILD
                     REALLGVAVFDGRITGEPLVNAELELIFHLLEQLGLAIKNIWLHDQLAANHEVMAEIL
                     RELSSACVVVSRDLAILHANKTAVRYFGRRAPRPSGELEFSDLPSPLSAKVYQVLRTG
                     AAIASFKFEREDAPGAIYRVSIVPFQRQPASLPASALLMAEDLTQSEQLQHLEIEAAK
                     LRLIKTMADRLTHEIGNAMVPLSTHQQLLADKWKDAEFRASLNAALADGVRRVTRLLN
                     QMRFLSRDSLGAQEAFPLSPLMDEAYQEACKHLPAKAAPLKHDHGRTPIILTGDRPAL
                     RHALAEVLLNALQASPAHPGAEVRSRMEAADNGQPGLRIEVRDPGAGFAPDDAQRATT
                     PFYTTRQVGLGLGLTVCRKIIETHQGKLEVVAPDSGQPGCVRIFLPVPASTPPPA"
BASE COUNT         1353 a         2404 c         3213 g         1374 t
ORIGIN      
        1 ggagggatgg acgacttgcg gatgatgtaa aaggacgttt caaggcggga gggagtggac
       61 gcgaacgttc ggcaaagccg gaacgggcgg ccgggtgttg tcgcgtgcct tggtttttcg
      121 gcgggatggg gccaaggctg gcggggacat ttcccctccc gggtggaggt tgccgcatag
      181 cgactgcttg cgttgccctc atcgcgggcg gcgtattcgc ccgagttggg tgaggttggt
      241 tggagattta tcttcgggct atgtgcgcag ggtgaatgat tgatgggatt ggttcgggaa
      301 cctgttgccg cgacgggggg aacgcttgac ggagggcgcc ggctatggca tttctggcgg
      361 cggcccgttg ctgacggggc gggcgaacag gacaggcaaa agcaaagcaa ctcggtatga
      421 agatgatgca ttggaactcc gttattccgg ccgcaagttg ggttttggcc gcgggtttgc
      481 tgctgctggc gggatgcgag cagaaaccga agggaccgca gccaacggtg atcaatggag
      541 tggaggttga cctggccaaa ttccaacagg catttttgaa cgccgcgccg gaagtgcaga
      601 ccagcgtttc ccgggtgcac ctggcggtgc gctacggaca gtatgcgcag gcggaggcga
      661 tggcgcgcaa gattgtccat ctccccggat tgacggaagc ccagcaggcg gtggcgcagg
      721 aagttcatcg ccagcttcag gagctggtcc gccggacgcc cacccggccg ccgccgtaag
      781 cggtgaggcg aggccgggcc gcccgggcgg atgcgggttc gcgccagccc cgggccggtc
      841 tcccggcttc cgggcgcggg acgccggcgg aagcccttgc cagggggcgc ggcccattct
      901 aagttcgcgc atggttttgc ccgcagatta tcacatgcat accccgctct gccgccatgc
      961 caccggcgag ccgtgggagt atgccgcgca ggcgcggcag ttgggtttgc cggaaattgg
     1021 ctttgccgat cacgggccga tgccgcggga cgactttgac gactggcgca tgaaggccgg
     1081 cgagctgggc gaatatgtgg cgcgggtcga gcgggcgcgg cgggatcatc cgcaactggt
     1141 catcaagctg gggctggagg tggattatct gccggggtgc gaggactgga ttcgggaact
     1201 ggcggggcgc catccctggg attatttcat cgggtccgtt cattaccttt cggagtcgtg
     1261 ggcgatagac aacccggcgc agcttgcccg gtggaaggag cgcgcgccgc tggaggtgtg
     1321 gacggcttac tttgagcgcc tgacgcaggc ggcggagtcg gggctttttg acattctcgg
     1381 gcatgtggat ttgtgcaaga agtttgccat ttatccgccg gaggattgca cgccgctgta
     1441 tcaccggttt ttgcaggcgg cccggcgcca cggggtggcg atcgagatca acaccgcggg
     1501 gctgcgcaag gattgccggg agctttaccc cggtttccac ctgctgcaat tagcggcgca
     1561 ggcgggggtg cccctgactt ttggctccga cgcccacgcg ccgaacgagg tggggctgaa
     1621 tttcgcggag gcggtggcgc aggcccgccg cgcgggctac acgcagtgct gccgttttac
     1681 ccggcggcaa cggcaggtgg cgccgctgcc gctcagttag aacgggccgc ggcgagccgc
     1741 cggtcaattt ccccgcgggt gggggccgag gtttgggcgc cgaggcgcgt gacggagagg
     1801 gccgccgccg cgttggcgaa gcgcgcggct tccagcaggg gtttgccctc ggcgagggcc
     1861 acggcgagag caccgttgaa cacatcgccg gcgccggtgg tgtccaccgg ccggaccttg
     1921 aagccgggga cccggccgcg ggcctggggg ccggcgacga acgcgccgcg cgcgcccagg
     1981 gtgaggatca cgttctgaac gccgcgcgcc agcagggcgt cggcggcgcg ggcggcggcg
     2041 gcttcgctgt ggaccgccat gccggtgagc aactgcgctt cactttcgtt gggggtgagg
     2101 agatacacat gccggagcag ggaggcgggc agaggccggg cgggggcggg gttgaggatg
     2161 acccgggcgc cggcgcgggc ggcgagtttg acggcggcgg tgacggtcgg gagcggggtt
     2221 tccagttgca ggacgaccac ggcggcgcgg cggaaggcgg cttgggcgcg ctgcaggtcg
     2281 gcgggcgcca gccgggcatt ggcgccggag gccacggcga tgctgttttc accgttccgg
     2341 gcgacgaaga tcaaggccac ccccgagggc gcggcggggt cgcgcaccac atgcttcaca
     2401 ttgatgccgt cggcgacaaa gccggcgagc gcctggtcgc cgaacatgtc gcggccgagg
     2461 cgcgcgatga aggtgaccgc gccgccggcg cgggcggccc cgacggcctg gttggcgcct
     2521 ttgccgccgg cggccagggc aaattcaccg ccgaggatgg tttcccccgg ctggggaatg
     2581 cgttggacct tgataatcat gtcggtgttg gagctgccga cgacgaggat ggaaggtttg
     2641 gtcatagcaa gcgtggcggg cggaggaggc gcgggggcgg tttagagttt cgaggcgacg
     2701 aggatggcca gccccagcaa gtagaaggcg aacatggcgg ccaggaactt gttggtgccg
     2761 ggcggggcgt ctttgaattc cttccagatg aacacgcccc agaaggcgcc gatcatggtt
     2821 gcgccctggc ccaggccgta agccagggcg gcgcccgcct tggtgctggc gatgatgctg
     2881 aaggccatgc cgaggttcca gatcatgccg ccgaggatgc cgatgaggtg caggcggatg
     2941 ttgcctttgg tgaagtaatc ggcgaaggcc accggggggc ccgagaaggg cttgaccatc
     3001 atgatgctgt tccaaatgaa gttggagagg agcaggccgg cggagaacag gacaatggcg
     3061 gtgtaggggc tgaggcggcc cggggtcagc acgtgggtat cggggtcaat cgttcccatt
     3121 gcctgggcga caaagctgta gaaccagccc atgagcaggc cggcggccac ggagatgacg
     3181 atgcctttgg cgggggtttt ctggccgccg gcggcgagcc gccggtaggc caggccatcg
     3241 aggatgatcg caatcacgat ggcggcgacg cccagggcga gcatgtggac gttgccctgg
     3301 tgggtggcga ggtaggtggt gatgacgccg agcaccagcg ccaggcccac gcccacgggg
     3361 aaggccaccg ccagcccggc gatgtcaatg gccgccacga gcaagatgtt ggacaggttg
     3421 aagatgacgc cgcccaggaa ggccgagccg agccatttgg gctcggcctg ctggaggtcg
     3481 gcgaggaagc cgcggccgcc gctgccggag ctgcccaggg taaaggccag gaggagcgag
     3541 agcagcagca cgcccacggc gtagtcccag tagaagagct ggaagcgcca ttccttgctg
     3601 gcgagtttct gggtgttggc ccaggagccc cagcagagca tggtgacgat gcagagggcg
     3661 acggcgagcg ggtaggattc gacgatgaac atacagggtt tcgggcaggt caggggttgg
     3721 cggcgggcga aggcttccgg gggcgggaga tttccggcgc ggcgcctttg tctctgaacg
     3781 cgcttgccct cgcgatgatc gttgtgcgtt tcacgttttc cggctccggg ccgcggccag
     3841 gaacggccgc gcgccggcat ttcccgcgca gtgtggcaaa gtccggcgcg tttgtccacc
     3901 cacagatttg ccggcttgag cgcttgtcct ggcgggcgcg ggtggtgtac tgtgggggga
     3961 tgaaattatt gcatggtttg gttggcggcg ttgtgctggg cgcggtggcg gggctgccgc
     4021 gggccgaggg ggcggagccg gtatggaaca ttcgcgacaa gccgctggag cgggtctcgg
     4081 tggtggcgca tcgcggggcg ggcgacctgg cgccggaaaa caccatggag gcgctggagc
     4141 tgtcgtggag catgggctgc attccggagc tggacgtgcg aaccaccaaa gatgggcacc
     4201 tgatgatgtt ccacgataag aacttcgcgc gcatcatgcc ccacgcgccg ccggagatga
     4261 agaagaagcg gctggaggac ctgacgtatg cggaagcgaa gcaactggac gtgggggcgt
     4321 ttcgcgggcc gcagtttgcg gggcagcgga ttgtgagcct ggccgagatt tgcgcggcgc
     4381 tgaagaaaga ccggcggcgc gcggcgtata ttgacgtcaa gaatgtggac ttcgccaaga
     4441 tggcccggga gacggcgggc gtgcatgagc agatcgtgct ggcgaccggc tcggaggcgg
     4501 attgcgcggc ctggaaggcg gtggcgccgc gcagcgaggc gtttttatgg gtcggcacct
     4561 ggggcgatac gaatgatgtg aacgtggaaa agcggtttgc gcggctgcgc gagattaact
     4621 ttgcgcacat agaccggctg caggtgcatt gccatttcaa caccaataat aaccgcatgg
     4681 tgcccagcga cgacttcatc cggcgcgcgg gggtggaggt gcgcgcgcgg ggaatcgagt
     4741 ttcagaccat gccgtggaat gtgccccagc tcgaagcggc ctacacccgg ctgctggacc
     4801 tgggcaccga ggggtttgga accgaccggc cggatgtggc gctgcgcgcg ctgcgcgcgt
     4861 attaccggaa cgggccgccg gactggaacg tccgggacca tattccccgg gcccggttca
     4921 ccgtgcaggc gcaccggggc gcgggcgacc tggcgccgga gggttcgaag gaggcgtttg
     4981 agctggggtg gaagctgggg tgcatcccgg aggcggattt gcgggagacc cgggacgggg
     5041 tgatcgtgtc tttccatgac aacaactttg cgcggatatt gcccaacgcg ccggaggagt
     5101 tgaagaagaa gggagtggcg gacctgacgt ttgccgaggt caaggaactg gacgtgggca
     5161 gcttccggaa ccagcaattt gcggggcagc gggtgatcag cctggccgag atggtgggga
     5221 ttctgaaagc gcatccggag cggtcgctgt acattgacat caagaaggtg gatttccaca
     5281 agctggccgc ggcgacggcg gaggtgcatc cgcagttgat cctggccagc acgaaatatg
     5341 atgaaatcaa gctgtggcgg gaggttgccc ccaaatcccg aaccctgcac tggatgggcg
     5401 gcccggccgc ctggatgagc gggccgccgg acgcgcccaa cggcccgcag gccaagctgg
     5461 cccagcggct gcaagcgctc cgcgaggtga acttcgccgg cattgaccag ctccagattc
     5521 acgtcaacac cagcccggag ggggtattct acccgagcga ggacttcctg cgcgaggcgg
     5581 ggagggagtt gcgcgcgcat ggcatcctgt tccagaccct gccctggaag cagaaagacg
     5641 ccaaggtttt ccatcggctg atggacctgg ggtgcgccag cttcgccacc gattatccgg
     5701 atgccgcgca ggcggcggtg agcgattatt accgggcgcg gtgaggcggc cgccgcgggg
     5761 cgcggcggag gcggcgcgcg cggggggagc cgcgccgggc cgccgggccg ctcaggcggg
     5821 gggcggagtg gaagcgggga cgggaaggaa aatccgcacg cagcccggct ggccggaatc
     5881 cggcgcgacg acttccagct tgccctggtg ggtttcgatg atcttgcggc agacggtcag
     5941 gcccaggccc aggccgacct ggcgcgtcgt gtagaacggc gtggttgccc gctgcgcgtc
     6001 gtccggggca aagccggcgc cggggtcgcg cacttcgatc cgcaatcccg gctgcccgtt
     6061 gtcggcggct tccatccgtg agcgcacctc cgccccgggg tgggccgggc tggcctggag
     6121 ggcgttgagc agcacctcgg ccagcgcgtg gcggagcgcc gggcggtcgc cggtcaggat
     6181 gatgggcgtc cgaccgtgat cgtgtttgag cggcgcggcc ttggcgggca ggtgtttgca
     6241 ggcttcctgg tacgcctcgt ccatcagcgg ggaaagcggg aaggcttcct gcgcgcccag
     6301 cgaatcgcgg gagaggaagc gcatttgatt cagcaggcgc gtcacgcgcc ggacgccgtc
     6361 ggccagggcc gcgttgagcg aggcgcggaa ctcggcgtcc ttccatttgt cggccagcaa
     6421 ttgctggtgg gtggacagcg ggaccatggc attgccgatt tcatgcgtca ggcggtcggc
     6481 catggttttg atcaggcgca gtttggccgc ctcgatttcg aggtgctgca actgttcgct
     6541 ttgcgtgagg tcttccgcca tgagcagcgc cgaggccggc aggctggccg gctgccgttg
     6601 gaacgggaca atgctgacgc ggtagatcgc gcccggcgcg tcttcccgct cgaatttgaa
     6661 gctggcgatc gcggcgccgg tccgcaggac ttgatacacc ttggcgctga ggggggacgg
     6721 caggtcgctg aactccagct ccccgctcgg gcgcggggcg cggcggccga agtagcggac
     6781 ggccgtcttg ttggcgtgga ggatggccag gtcccggctg acgaccacgc acgcgctgct
     6841 gagttcgcgc aggatttccg ccatgacttc gtggttggcg gccaactggt cgtggagcca
     6901 gatgttcttg atggccagcc ccagttgttc gagcaggtgg aagatcagct ccagctcggc
     6961 gttcacgagg ggctcgccgg tgatgcgccc gtcgaagacc gccaccccca gcaacgcctc
     7021 ccggtccagg atcggcacgg ccacttgcgc ccccagcagc tcgaactcat tccgggtttc
     7081 ggggtcatgg cgcgcctcct cgctgttgcg ccgcaggatg cggccgaggc gcaggaggtg
     7141 tccgccgatg ccgccttcga gcgacagctc gaagtggtgc agcaagctgc gcgagatgcc
     7201 gagggagccc gccgcgtgca ggcgccggct ttcggcctgc gcgggggcgt ggccgaaggc
     7261 ggtgagcggc gggcgcagga agatggccgc gcggttaagg ccgagaatct cgcgcagcag
     7321 caagaggaat tgctgcagca tgccctcggt gttcagcgag tgcgtcagga tgcctgaaaa
     7381 gttccgcagg acgctcagcg tcggaacggc gcccgcgggg gctgccttct cgatcggcgc
     7441 cggggcctcg ggcggcggcg gcgcgggcaa aggcgcgggg cgcgccgccg cggacttcgg
     7501 ccacaaccgc tccagcaggg cggcgaggag gcgcccgcgg acgggtttgg tcaggatgtg
     7561 cgtggcgcct tgcaggtagg cttcctcttc ccattccggc tgtttggcgc cggcataaat
     7621 gatgatcggg caccggggat cccggcgccg cagcttttcc agcagccaga tgccctgcac
     7681 gccggtcagc tcaaaatcca cgatgcaggc ctcggccagg ccgtgcgcca gaaagggttc
     7741 cgcctcttcg aggttcgccc ggtccagcac ccggtacagg tcgggattga gggccgcgcg
     7801 aatcgcttcc gtcacatccg gatgtgcgcc caggaccagc agggttttca tggcccgttt
     7861 cctccggcgg gttcggcgcg gcgcggacgg cgggccggcg gggccgaggc gttcgccgcc
     7921 gccggaagca ccgcgttctc cctcccggca gaccgcggaa ggtcatcccg cgggagcaga
     7981 tccgcacccc cggcacccgt gctcgcgata ttattcatca ttccgcagtc ggaactgcgc
     8041 tatcgtggct atcagactta ccatttttgc gaggcaaaga caacaacagc gactacggat
     8101 gtgcagcccg ggaatatcgc gcattttaac ccggggtttc tccgggttgg cgggaggggt
     8161 gaaggggagg aatcgcttgc gggtgggcgg gtgggaggct ttctacttta gaggcggcta
     8221 agcgggcagg atgcccgcgc gccgggagag gcgggggtga aagtgccgcg tggaggcacg
     8281 cggccttcta aggtagtgtc ctaaaagttc tgcaaaaagc agcgggtcat ggtaagccac
     8341 tggg
//
LOCUS       DAOPQG010000138         8036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_10034_length_8036_cov_13.023556, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000138 DAOPQG010000000
VERSION     DAOPQG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_8036_cov_13.023556"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2557
                     /locus_tag="PKI07_12500"
     CDS             <1..2557
                     /locus_tag="PKI07_12500"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOC51905.1"
                     /translation="NMNTLLETIASNLLRLGFVPWFFGVGLQSVAVLAAAGGLCLLLR
                     RAAAATRHWIWFLAVASLPCLMLLAMAPPVWQKPLWGVATDLNSGNQFSLTLTLGPDN
                     ERVNAPKPSPPETTAATAVNGTGRRAAEPVTAQFRTTWLALAVIVWLPGAVWGLASVL
                     AGQLQLRRLARSATPLLSETRTAVSQNRLPGNEAAKNTSPPAGEEERGAGDADWALLL
                     KEVGERLRLRRPVRLLLARDNLMPVTWGWWRPVVLLPAEAAEWPAGRRRVVLLHELAH
                     AKRWDCLTQIITRVVCALYWLNPLVWVAARRMSIERERASDDWVLRSGYRASDYAAHL
                     VAIARRFQWQPRLTGIAMARSSQLRGRVAAIVDASRARGLRPATALGIAILMSTIALS
                     VSGRSPAATRSPGEDAALRREQFDRLQSFIRVKEQQVRELSTAVGERIPPEIRRFFAA
                     ARREDWRAMTNQVEHLILPYQQHQRGTNPPLEVSRTVYRQPVMEIKLAYDHVANCTPK
                     YTALLADGIISSIPAGSIYFGGTDPGRGVPTAFCQSHAEGNPFFTLTQNALADGTYLD
                     YLRAMYGSKIHLPDYADAQKCFQEYLTDAQQRLKENRLKPGEKVEQVGNHQVRVGGKV
                     AVMCINGLLAKVIFDRNPEREFYLEESFPLDWTYPHLVPHGLILKLNRQPLAQLPEDV
                     LARDHEYWSKLVAGMLGDWLNEKTTVREINDFVDRIYVRKDRQGFSGDPLFIQNDYAG
                     KIFSKLRSSIGGIYAWRLNPDLDVCPAEYHPQSEAERQRLSQEADFAFRQAFALCPYN
                     PEGMLRYAQFLMQMERFDDALRVAETCLKMGAPDDQLRALIDDIKSRQKRMSE"
     gene            3052..4899
                     /locus_tag="PKI07_12505"
     CDS             3052..4899
                     /locus_tag="PKI07_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOC51906.1"
                     /translation="MADYPTTRAGRGRMLPRIGSPAYHLLLATVAILILGPLGGISAA
                     FMNFSIGFFIGGQVLAGILGSTVTLPYGPEGRHGANYMQTMAASVAGMSGMAVLAQAM
                     VWLGLPEPPAWKLALYFMSIGMFGVGVGMLYTPVLVDRMQLAYPSGFAVANILRALTD
                     PNLLKRSVAKLGGGMLTGYAGGLFALKLPALAGLGMSPSSIAALTRLSLSPSTFGAGL
                     IVGARIAVPALVVALIGKWQTPRLVSLGWLEPGEPFRKIGFIIALGTILGAAVLDVAL
                     ILIQAARRFRLKSGPVEGPAADWKRVNLFRLVLWVGFWGAATVFFGSQVLRQPVFFLA
                     VAVGLSFLFVLVNGISLGISDWNPISSAFVLTVFILAALGLRDPGVGLLCAAILLIAC
                     TEGGDMQQDRSTGWRLGTNRVTQFRYQVIGIVMGAALAVVLAKVFMHSYPVLRVDQFT
                     HPHTGGAQQWQSAMTYKLVGALNGITTSQPQVMRALRLGVLLGLAIEIARKLIKRVPA
                     YQRALKTSARARAGNFLLDAVLLPSPYASAFGGFVELPTVLWWTAGGVGSSVFDYAQA
                     RRALRRPPAAAGSLPPDMSTMSLIGGGLIAGDSLAALSVGLYGLIRTVL"
     gene            4929..6392
                     /locus_tag="PKI07_12510"
     CDS             4929..6392
                     /locus_tag="PKI07_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51907.1"
                     /translation="MSQPTSPSPAGAKSAGELMVAYFRDFGVLKETRKEYWGIQVVNF
                     LDCTFYFALLTIATVFLSEDLGLNDRWAGYSTAVFTSATSLMLFVSGMYTDWLGIRRS
                     LILSMAAMLVLRLGVLGVGLAPGLPHRGLIASVLLLLMAPFMAAIQTVFQSACRRFTT
                     RRSRSAGFNLWYLFMNIGAAAAGFSIDIVRKLLHLPNAHIFTMGVVLAALCLGVGVTI
                     IRREDQLVGPDEPPEAKEREARVERKPPLQAIREVVREPTLWRLLVLIGLILGVRAVY
                     AYLYLLMPKYWLRTIGPDAAIGTLNAINPIGIVIGLILFIPIANKFNVFSMLVYGAMV
                     SALSLFPLALPWQWYGADIARAHYLMALASMVIVTVGEVAWSPKLYEYTAAIAPRGQE
                     GVYLGLSLIPWFVAKTLVSAFSGHMLEYWSPEKVSVGGVSLPLQQALLQNQVPYWHGP
                     AAMWLVLGGYAFAGCVIAMLLRGWLTRGAHWKASPPA"
     gene            6619..6780
                     /locus_tag="PKI07_12515"
     CDS             6619..6780
                     /locus_tag="PKI07_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51908.1"
                     /translation="MSILDLIKATVVCGAIAFLIYSYPVVGQIVLIGFLSLLWLLYAH
                     RTIVHLRRR"
     gene            6922..7614
                     /locus_tag="PKI07_12520"
     CDS             6922..7614
                     /locus_tag="PKI07_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOC51909.1"
                     /translation="MDLETNVRAVRQRIEAACARAGRDPNEITLVAVTKGQPPESVCA
                     AAALGLTLFGENKVQEAKAKIPLCPGRARWHMVGHLQTNKCRDAVGLFEMIQSVDSLR
                     LAEELNRRAEQAAKTMPILLEVNAVGEASKFGCRPDQLLADLDRINALPRLEIQGLMT
                     VPPWAIDPEKVRPVFRQMRELKARCEQLLGAPLPHLSMGMTGDFEVAIEEGATLVRIG
                     TALFGARPRAGA"
BASE COUNT         1300 a         2425 c         2750 g         1561 t
ORIGIN      
        1 taatatgaac acgttgcttg aaacgatcgc gagcaacctg ctgcgcctgg gattcgtccc
       61 gtggttcttt ggcgtcggac tgcagagcgt cgcagtgctg gccgcggcgg gcggtttgtg
      121 cctgctgttg cggcgggcgg cggcggcgac ccggcattgg atttggtttc tggcggtcgc
      181 cagtttgccc tgcctgatgc tgctggccat ggcgccgccc gtctggcaaa agccgctttg
      241 gggggttgcc actgatttga actccggcaa ccagttttca cttacgctga cgctggggcc
      301 ggataatgaa cgggtcaacg cccccaagcc gtcgccgccg gaaaccacag cggccaccgc
      361 ggtcaacgga accggacggc gggccgccga accggtcacc gcgcagttcc ggactacgtg
      421 gctggcgctg gccgttatag tctggctgcc gggagcggtc tgggggctgg cttccgtgct
      481 ggcagggcag cttcagttgc gccgcctggc gcgcagcgcc actcccctct tgagcgaaac
      541 gcgcaccgcc gtctcccaga atcgtttgcc ggggaatgag gcggcgaaaa acacgtcccc
      601 gcccgcgggg gaggaggagc gcggggccgg ggatgccgac tgggcgctcc ttttgaaaga
      661 agttggggaa cggctgcggt tgcggcggcc cgtgcggctg ttgctggcca gagacaattt
      721 gatgccggta acgtggggtt ggtggcggcc ggtcgtgctg ctgccggcgg aggcggccga
      781 gtggccggcc gggcggcggc gggttgtgct gttgcacgaa ctggcccatg ccaaacgctg
      841 ggattgcctg acgcagatca ttacacgggt tgtttgcgct ctctactggc ttaatcctct
      901 ggtttgggtg gccgcgcgga gaatgagtat tgaacgcgag cgggccagtg acgactgggt
      961 gctgcgcagc gggtatcgag cttcggatta tgccgcgcac ctggtagcga tcgcccgtcg
     1021 tttccagtgg cagccgcggt taaccggcat tgccatggcg cgctcgtccc aactgcgcgg
     1081 gcgggtggcg gcgatcgtgg acgcctcgcg tgcgcgcggg ctgcggcccg ccaccgccct
     1141 ggggatagct attctcatga gcacgatcgc cttgtcggtg agcgggcgca gtcccgccgc
     1201 cacccgcagt cccggggagg acgcggctct ccgccgggag cagtttgacc ggctgcaatc
     1261 ttttatccgg gtcaaggagc agcaggtgcg ggaactgtcc accgcggtgg gcgagcggat
     1321 tcctccggaa attcggcgct tcttcgcggc cgcccgccgg gaagattggc gggccatgac
     1381 caaccaggtt gagcatctca tactgccata ccagcaacac caacggggaa ccaatccccc
     1441 cctcgaagtt tcccggaccg tttaccggca gccggtgatg gagattaaac tggcctacga
     1501 tcacgtcgcc aactgcaccc ccaaatatac ggcgctcctg gccgacggca ttattagctc
     1561 catcccggcg ggcagcatct attttggcgg caccgacccg ggccgcggtg tgccgacagc
     1621 cttctgtcag tcccacgcgg agggcaatcc gttcttcacg ctgactcaga acgccctggc
     1681 cgatggaacc tatctggact atttgcgcgc catgtatgga agcaaaatcc acctgccgga
     1741 ctatgccgac gcgcagaaat gcttccagga atacctgacc gacgcacagc agcggctgaa
     1801 agagaaccgg ctcaagccgg gggaaaaggt cgagcaggtg ggcaaccatc aagtgcgggt
     1861 gggtggcaag gtcgccgtca tgtgcatcaa cggcctgctc gccaaggtta tctttgaccg
     1921 gaatccggag cgggagttct atctcgagga aagttttccg ctggattgga cgtatccgca
     1981 cctggtgcca cacgggctga tcctgaagct caaccgccag ccactggcgc agttgccgga
     2041 ggacgtgctc gcccgggatc acgaatactg gagcaagctg gtcgccggca tgttggggga
     2101 ttggcttaac gaaaagacga cggtgcgcga aatcaacgac tttgttgacc ggatttatgt
     2161 gcggaaggat cgccagggat tctccggcga cccgcttttc attcaaaacg attacgccgg
     2221 gaagatattc tcgaaactgc gaagttccat cggcggcatt tacgcctggc ggttgaatcc
     2281 cgacctcgat gtctgcccgg cggaatatca tccccaatcg gaagccgaac ggcaacgcct
     2341 gagccaggag gcggatttcg ctttccgcca ggccttcgcc ctttgtccct acaacccgga
     2401 agggatgctt cggtatgccc aattcctgat gcaaatggag cggtttgacg acgcgctgcg
     2461 ggtggcggag acatgcctga aaatgggcgc gcccgacgac cagttgcggg cattaataga
     2521 tgatattaag agccgccaga agcggatgtc ggaataaagg cggccccgaa ccgcttgtcg
     2581 ccaaaggaca aaaggcgcag gcggatgggc aaacgaatat cagcaaccag cggaagccag
     2641 cctccccgta atccccagcg cgggatatgt ccaggtccgc atccgcctgg gcgcgtgcgc
     2701 gaaggtaccc ggcgcagggc gaggatccgg atacgaccgc gccgcactca aggccagtcg
     2761 caagtcctcc cacgacgccg ctgacggcct tgacatcctc ccgcctgcgg ttttccggtg
     2821 ctgccacggt tcgctatgag ttggcggtgc gccgcggttt ccgcgccgcg accgggcggc
     2881 ctagtcccgc ggccaacgcc gccccccgct tttccggcgc cgaactgccg ccgtatgcct
     2941 ccaggctttg cgctgccgtt aaaagcgggg agacgcccca ggttatgggg catggtgttt
     3001 tgctttccct gcggcgcggg cttgcctatc ttccggcgca ttcgtcgcgc gatggctgat
     3061 tatccaacaa ctcgtgccgg cagggggcga atgctcccgc ggatcggttc gccggcctac
     3121 cacctgttgc tggcgacggt ggcgatttta atcctggggc cgctgggcgg gatttccgcg
     3181 gcgttcatga acttttcaat cggcttcttc attggcggcc aggtgctggc gggcatcctg
     3241 ggcagcaccg tgaccctgcc ttacgggccg gaagggcggc acggggcgaa ttatatgcag
     3301 acgatggcgg cgtcggtggc gggcatgagc ggcatggcgg tgctggcgca ggcgatggta
     3361 tggctggggt tgcccgaacc gccggcgtgg aagctggcgc tgtatttcat gtccatcggg
     3421 atgttcggcg tcggggttgg aatgctctat acgccggtgc tggtggaccg gatgcaactg
     3481 gcctacccgt ccggcttcgc ggtggccaac attctgcggg cgctgacgga cccgaacctg
     3541 ttaaagcggt cggtggcgaa gctgggcggc ggaatgctga cgggctatgc gggcggattg
     3601 ttcgcgctca agttgccggc gctggcgggg ctgggaatgt cgccgtccag catcgcggcg
     3661 ctgacccggt taagcctttc gccttcgaca ttcggggcgg ggttgattgt gggagcgcgg
     3721 atcgcggtgc cggctttggt ggtggccctg atcggcaagt ggcaaacgcc gcgcctggta
     3781 agcctcggct ggctcgagcc cggcgagccg ttccggaaaa ttggcttcat catcgcgctg
     3841 gggaccatcc tcggggcggc ggtcctggac gtggcgctga ttctgatcca ggcggcgcgg
     3901 cggttccggc tgaaaagcgg cccggtggag gggccggcgg cggattggaa gcgggtcaac
     3961 ctgtttcggc tggtgctgtg ggtggggttc tggggcgcgg cgacggtttt cttcggcagc
     4021 caggtgctgc gccaaccggt gttcttcctg gccgtcgccg tgggactgag ttttctgttt
     4081 gtgctggtga acggcatttc gctgggaatc tcggattgga acccgatttc cagcgcgttt
     4141 gtgctgacgg tattcatcct ggcggcgctg gggctgcgcg atcccggggt gggcctgctt
     4201 tgcgcggcca ttctgctgat tgcctgcacc gaagggggcg atatgcaaca ggatcgctcg
     4261 accggctggc ggctggggac gaaccgcgta acgcagtttc gttatcaggt tatcggcatc
     4321 gtaatgggcg cggcgctggc ggtggtgctg gcgaaagtct ttatgcactc gtatccggtg
     4381 ctgcgcgtgg accagttcac ccatccgcat accgggggcg cgcaacagtg gcaatcagcc
     4441 atgacctaca agctggtcgg cgcgctcaac ggcatcacca cctcccagcc gcaggtgatg
     4501 cgggcgctgc ggctgggcgt gctgctcggc ctggcgatcg agatcgcgcg caaattgatc
     4561 aagcgcgtcc cggcctatca acgggcgctg aaaacctccg cccgcgcgcg cgcgggcaat
     4621 ttcctgctgg acgcggtgct gctgcccagc ccttacgcct cggcgttcgg cgggtttgtc
     4681 gagttgccca cggtgttgtg gtggacggcg ggcggcgtgg ggtcttcggt gtttgattac
     4741 gcgcaggcgc ggcgggcttt gcgccgtccc ccggcggccg ccggatcgtt gccgccggat
     4801 atgagcacca tgtcgctgat cggcggcggc ctgatcgcgg gcgattcgct ggccgcgttg
     4861 agcgtgggcc tgtatggctt gatccggacg gtgctatgac gccctgagcg tgctatagaa
     4921 caataaatat gagccagcca acttcgccgt cgcccgcggg cgccaaatcc gccggggagc
     4981 tgatggttgc ttactttcgg gattttggcg tgctgaaaga aacccgcaaa gagtattggg
     5041 gcatccaggt ggtgaatttc ctggactgca ccttttactt tgcgctgctg accatcgcga
     5101 cggtgttttt gtcggaagac ctggggctga acgaccggtg ggcgggctat tccacggcgg
     5161 tcttcacctc ggcgacgtcg ctgatgttgt ttgtttcggg gatgtacacg gactggctgg
     5221 ggatccggcg ttccctgatc ctttcgatgg cggcgatgct ggtgttgcgg ctgggcgtgc
     5281 tgggggtggg gctggccccg ggcctgccgc accgggggct gatcgccagc gtactgctgc
     5341 tgttgatggc ccccttcatg gcggcgattc aaaccgtgtt tcaatccgct tgccggcggt
     5401 tcacgacccg gcgctcgcgc agcgcgggat tcaacctctg gtacctgttc atgaacatcg
     5461 gcgcggcggc agccggcttc agcattgaca tcgtgcgcaa gctgctgcat ttgcccaacg
     5521 cccacatctt cacgatgggc gtggtgctgg cggcgctgtg cctgggggtg ggggtgacga
     5581 ttatccggcg cgaagatcaa ctcgtggggc cggacgagcc gccggaagcg aaggaacggg
     5641 aagcccgggt cgagcgcaag ccgccgctgc aggcaattcg cgaggtggtg cgggaaccaa
     5701 cgctctggcg gttgctggtg ctgatcgggc tgatcctggg cgtgcgcgcc gtttatgcgt
     5761 atttgtatct gctcatgccg aagtattggc tgcgaacgat cggcccggac gcggccatcg
     5821 gcacgctcaa tgccatcaat cccatcggca ttgtgatcgg gctgatcctc ttcatcccca
     5881 tcgccaacaa gttcaacgtg ttcagcatgc tcgtttatgg cgcgatggtg tcggcgcttt
     5941 ccttgtttcc gctggcgctg ccgtggcagt ggtatggcgc tgacatcgcc cgggcgcact
     6001 atctgatggc gctggcaagc atggtgattg tgacggtggg cgaagttgcg tggtcgccca
     6061 aactttatga gtacaccgcc gccattgcgc cccggggcca ggaaggggtg tacctcggcc
     6121 tgtcgctcat cccgtggttc gtggcgaaaa ccctggtcag cgcgttctcg gggcacatgc
     6181 tggaatactg gtcgcccgag aaagtttccg tgggaggcgt gagcctgccg ctgcaacagg
     6241 ccctgctgca aaaccaggtt ccctactggc atggcccggc cgccatgtgg ctcgtgctgg
     6301 gaggatatgc ctttgcggga tgcgtaatcg caatgctgct tcgcggctgg ctgactcgcg
     6361 gcgcgcactg gaaggcctct ccgcccgcgt aaagccttcg cccgctcgaa ctcccgcata
     6421 atccgcgtgc cgatccggtc tcccggaagc ggaaaacgcc ttcggcggaa ccccatcctt
     6481 tcccggctgc gatgcttgcc gtttggcata agagttgcct tcgtttatgg tagaatgtag
     6541 tggcgttcag gcgagcggag gcggtttggc gcaggatgcc agccgccgcg gcccgcgccc
     6601 gaagccgaac actgtgttat gtctattctt gatttgatta aagccaccgt ggtttgcggc
     6661 gcaatcgcct ttctcatata cagttacccg gttgtgggcc aaatcgtgct catcgggttc
     6721 ctgagcttgc tgtggctgct ttacgctcac cggacgatcg tccatctccg ccgccgatag
     6781 cccctccggc gccgccgccc ggcggagacc gcggggcccg ccgcgggaat gatcggaacc
     6841 cggggaaatg acccgccccg gggaaacgcg ccccctcact attcgctatt ctctgatttc
     6901 gattccggtt atcgtgcgcg catggatttg gaaacaaacg tgagggcagt ccggcagcgc
     6961 atcgaggccg cctgcgcgcg cgcggggcgc gatcccaacg aaatcaccct ggtggcggtg
     7021 accaaaggcc agccgcccga aagtgtctgc gccgccgccg cgctgggact gaccctcttc
     7081 ggcgaaaaca aggtgcagga ggccaaggcc aagattccac tgtgcccggg ccgcgcgcgc
     7141 tggcacatgg tcggccattt gcagaccaac aaatgccgcg acgccgtggg gcttttcgag
     7201 atgatccaaa gcgtggacag cctgcgcctg gccgaggagt tgaaccggcg ggcggaacag
     7261 gcggccaaaa caatgcccat tctgctggaa gtcaacgcgg tgggcgaggc cagcaagttt
     7321 ggctgccgcc cggatcagtt gctggcggac ctggaccgca tcaacgcgct gccgcggctg
     7381 gaaatccagg gcctgatgac cgtgcccccg tgggcgattg acccggaaaa ggtgcggccg
     7441 gtctttcgcc aaatgcgcga gctcaaggcg cgctgcgagc agctcctggg cgcgccattg
     7501 ccgcatctaa gcatgggcat gacgggcgat ttcgaggtag ccatcgagga gggggcaacc
     7561 ctggtgcgca tcggcacggc cttgttcggc gcccggccgc gggctggcgc gtgaactgac
     7621 cgtccggacg gcgccagggc ctttgctttt atagatgatg agccggggag gttgagcccg
     7681 cgggttgggc ggcggttcag cccttcgaag aaagtgcctg gccgtgcgcc caggccccgg
     7741 gcagttggaa acagacgggg ctggctaact ttgcccggaa ggagacagtc gccgcccctt
     7801 tgcccgatct tccgggccaa tttgcgctgg ccgtgcaacg gatcggcggt gatgcgcttg
     7861 ccattacaga aactcagccg ccgataacaa aggaccctgc ggccgcagac aatccgggcg
     7921 ggcacgcagg tgtttttctc agccgccggg cgaacggccc ccgagcgccg cgtgagggca
     7981 cgcggcctgc aggaaatgga agcacgccgc ggccgacccc atgccctctc gcagca
//
LOCUS       DAOPQG010000139         7918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_10236_length_7918_cov_11.580440, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000139 DAOPQG010000000
VERSION     DAOPQG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10236_length_7918_cov_11.580440"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..799)
                     /locus_tag="PKI07_12525"
     CDS             complement(374..799)
                     /locus_tag="PKI07_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51910.1"
                     /translation="MNFDLRRQGSRGPDGPHPAPGSICSSPSLAGPLARHSRTRRSAS
                     GKTFRRALAWNWVGILAAGLWVIGCSKQSATEQPEATMDELNRAARAWGMINPAPLVH
                     VSDLTNFPTLHGKRLPTPEAGKKLAIDPRTRQVIIVDDR"
     gene            complement(789..2168)
                     /locus_tag="PKI07_12530"
     CDS             complement(789..2168)
                     /locus_tag="PKI07_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HOC51911.1"
                     /translation="MKPFKSLAITSPAELCFGVSPKPLKTRDGLTIGGGIVYPELNFT
                     LPAMFVDANTMPEVRQHYRDIITGACQRAAELETPGLVVEFETLPPMTENPAWGIELT
                     RILLEGMAEARGRYGLRSVLRITPNDTREMERPPRMRSGQYYEAMMQTFDGCAAAGAE
                     LLSIESVGGKEVHDEALMNGDIRAVIFALCVLGVRDMKFLWTELNKIAAKHGVHCAGD
                     TACGFGNTAMVLAEQKMIPRVFAAVVRAITAVRTLVCYECGAVGPGKDCGYENPILKA
                     ITGFPMAMEGKTAACAHLSPVGNIAAAACDTWSNESVQNLKLLGGMAPTCYVEQLIYD
                     CRLMNQALADGRQAALLYQKWMVNSDASRDPQAWVLSPESAIAIAQSIVTAPGPYAAG
                     KAAALTAVRLLRNAHQEKRLALHPRELPWLDRIQETVEELPSAEADFISEMMGAVDTE
                     KFVVQDYEL"
     gene            complement(2181..2813)
                     /locus_tag="PKI07_12535"
     CDS             complement(2181..2813)
                     /locus_tag="PKI07_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOC51912.1"
                     /translation="MSELKQLHDAVLNGDAKTARVVTEKALADGVEPLKMVQDYMMPA
                     MAEVGRRFESNEYFVPELLLSARAMKAALELLRPLLVASGAKSTGRVVIGTVKGDLHD
                     IGKNLVTALLEGGGFEVFDLGVNVSPEQFVMAVKEKGAQIVAMSALRTTTMPAMKTTL
                     DALNQAGVRGQVKVLVGGAPITQTYADQIGADGYSASAAGAVAVAKSVLA"
     gene            complement(2891..3760)
                     /locus_tag="PKI07_12540"
     CDS             complement(2891..3760)
                     /locus_tag="PKI07_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51913.1"
                     /translation="MKARICHSQAGAAPHRSQGGGFTLIELLVVIAIIAILASLLLPA
                     LGKAKEKAKSISCLNNMKQVGLATKLYLDDNSGKLLPLWRQPGAPGWDPWIYDPPTFV
                     VQNPYGLFWQDALRLGGYAKARKVFDCPSMRWLAARERGGSVSTNNTLEIGMNHPEYA
                     VTLPATTVSPKMRAESQVSMPSASVVFGDAGGVTLASKNLNPDRWVEDQEWDVLLGVI
                     GTGCSYFRVPTDGDFSAEPTRTVPRHSGRLNVAHFDGHAEPIKNSKLGYTLPRTSDGA
                     LWARDHRSLMLVP"
     gene            complement(3779..7912)
                     /locus_tag="PKI07_12545"
     CDS             complement(3779..7912)
                     /locus_tag="PKI07_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOC51914.1"
                     /translation="MLLIAVSASAASRTWNGGASPDPNWSTAGNWVGGVAPVNGDDVI
                     FINAAGDPLTSSVDVVRSVNSITRNATDAAASLMAVNTLNHLTVNNHILSSGGMTIAG
                     PISLAHDLNIWRASGTGTLLFTNAPGDTVGISGAHQISFTNVLGGSPGAFLLMANPLS
                     TWSGGAYIRAGNVRVGDVSSTGSPGAPTAGPLGTGTVTIGVPGSTDFGVLSFQGSGNL
                     TLHNNVLVNSSGGVANSRRLVNNLTPPLKLTLNGAITNNEDIYLTAIAGNTIRLDGTI
                     RGTGGLVANSAGLNALLTGGNSYAGGTVVLGGANLGFGSDTALGSGTVAFGATAGEGQ
                     IFSYAVNGPRAINNNVIIRTVRYIASNGTIDGLAGNDQTFNGTVTLDQGPTDVRDIYC
                     QANMTINGTLAGATGDAALRKIGGGTLYLKGANSYGGGSYILGGTLNINSDAALGADP
                     GWPTPNLTFAGGGGTLQVGAASVALSANRNISLDPGVSGTLDTGGNSLIVNGVISGGG
                     HLFKTGSGTLTLTAAGNDIAGYVFIDGNVLVDGGSFYNGLEFVVGNTAPGTLTINGAT
                     ASAQSITVRPGTDSSFNLVGGTLSVDQLFAVAPANVNAIATFDGGTITPWGIQPARFD
                     NFISGLSHAYLKSGGLILNSGGQTLTIPQALEHDPALGGAPDGGFTKVGAGEVYLTGT
                     NTYTGPTMADGGRLVVTTAGGSKGDYTVTDTSTLRVKVSYPGSSLTNASLSFGLFMYS
                     TLEIDLNNLGLPTVAPIQTSGALTPGGVANIVVANLASPVPGVFPLIKYGSLDGGGFA
                     SLMLAPIPNVTAMLSNDVAHSVIDLVITEVNYPKWKGNVNNDWDISATPNWVGIGNGS
                     PTVYEEPSVPGVPVWFDDTADSGSVNLTTTLSPYSMTASNSSLNYSFSGVGSLSGPMG
                     LIKKGSAQLTMMTANTYYGANIISGGSVIAAAPNALGSGGALAINGATLNIGDHNQAA
                     GQVTLANGNILGTNGVLAASGYTLASGVVGAHLANGNLTKVGPGTVSLMASNNYGVTE
                     IQDGILRVTDSSALSPGGFSGTTLTVIDVGGALLFDGTISTDEHFYLAGAGPDGLGAL
                     RVTNGTVTIAQHGFMPTNATINVAPGATLVRGGSLYTVVTNLLTLTKRGAGDFWEWGT
                     VSTYIPLDVVEGTLGVAGTVSGSVTVRSSGTLSGSGAIGGPVVIEGGGTVAPGASIAT
                     LTINDSLTLQGTARMEIAKTGLVTANDKVEGMTTVIYGGNLVVTNLGPDALEAGDTFQ
                     LFAAAARSGTFASLSLPPLDPGLAWANNLAVDGTIQVVSSAPYLGGIVKLNDGNFQLS
                     GTGPDNAAYRVFASTNVAQPLNDWVQVGTGTFINGVLDFTDLQSTNYARRFYRVVTP"
BASE COUNT         1619 a         2468 c         2265 g         1566 t
ORIGIN      
        1 cccagcatac cacgccaaac gacattgtaa agagttatct gttacataac actagctggc
       61 ggcgcccgcc atcttcggcc gcccgcgcct gcctcgccca acagccgaaa cgtcgcgacc
      121 ccaatgctga aacaggggcg ggccgggcag ccagcgcaga ggcaaacgac gggccgctat
      181 ttcacctttg gcgccgcggg cggcatgcgc tggcctcaac cggcacaggc agatcgcagc
      241 gatcgagatg gatggctctc cccaaccgcc tgcgtgaaag cggcggaatg agccggaaac
      301 gagaaggaaa cgatccttgc ggccgccgcg agtcgcgctt tatttcctgc gcgccgcggt
      361 ttggaagaag gctttaccga tcgtcaacga ttattacttg gcgggtgcgg ggatcaatcg
      421 ccagcttttt gcccgcctcg ggagtgggca ggcgtttgcc gtgcaaggtc gggaaattcg
      481 tcagatcact gacatgaacg agcggggcgg gattaatcat tccccaggcg cgggccgccc
      541 ggttcaattc gtccatcgtt gcttccggct gttccgtggc gctctgcttc gagcaaccga
      601 tcacccataa accggcggcc aggattccga cccaattcca tgccagcgcc cggcggaatg
      661 ttttgccgga agcggatcgc cgggttcggg aatgccgcgc cagcgggcct gcgaggctgg
      721 gggaggaaca aattgatccc ggggccggat gcggcccatc gggtcctcgc gaaccctgcc
      781 ggcggaggtc aaagttcata atcttgcacg acgaattttt ccgtgtccac ggcgcccatc
      841 atttcgctga tgaaatcagc ctcggcggaa ggcaattcct ccacggtttc ctggatgcgg
      901 tccagccacg gcaactcgcg cgggtgcagg gcgagacgct tctcctggtg tgcgttccgg
      961 agcaaccgca cggcggtaag cgcggccgcc ttgccggccg cgtaggggcc gggcgccgtg
     1021 acgatggatt gcgcaatggc gatggcgctt tcaggcgaaa gcacccacgc ctgcggatcg
     1081 cgcgaagcgt cagagtttac catccatttc tggtagagca acgccgcctg gcggccgtcg
     1141 gccagcgcct ggttcatgag gcggcaatcg tagatgagtt gctcgacgta gcaggtcggg
     1201 gccatgccgc ccagcagctt gaggttctgc acggactcgt tgctccaggt atcgcaggcc
     1261 gcggcggcga tattgccgac cgggctcagg tgggcgcaag cggcggtctt gccctccatc
     1321 gccatgggaa aaccggtaat ggctttcaga atcggatttt catagccgca atccttgcca
     1381 ggcccgaccg cgccgcattc gtagcatacg agcgtgcgca ccgccgtgat cgcccggacc
     1441 acggccgcaa atacccgggg aatcatcttt tgctccgcca gcaccatcgc cgtgttgcca
     1501 aagccgcagg cggtgtcacc ggcgcagtgg actccgtgct tcgcggcaat tttgttcaac
     1561 tcggtccaga gaaacttcat atcgcgcacc cccagcacgc agagcgcgaa tatcacggcg
     1621 cgaatgtcgc cgttcatcag ggcctcatca tgcacctcct tgccgccgac cgattcgatg
     1681 ctcaacaact ccgcgccggc cgccgcgcag ccatcgaaag tctgcatcat cgcctcgtag
     1741 tactggccac tccgcatgcg tggagggcgt tccatttccc gggtgtcatt gggggtgatg
     1801 cgtaaaacac tccggagacc gtaccgcccg cgcgcttcag ccattccctc gaggagaatc
     1861 cgcgtcaact cgatgcccca ggcggggttt tctgtcatcg ggggcagggt ttcaaactca
     1921 acgaccagtc ccggggtctc caactccgcc gcccgctggc atgccccggt gataatatcc
     1981 cggtaatgct gtctcacctc cggcatcgta ttcgcgtcca cgaacatcgc cggcagggtg
     2041 aagttcagct ccggatagac tattccgccg ccaatcgtga gcccatcgcg ggtcttcagc
     2101 ggtttggggg acacgccaaa acacagttcg gcgggactgg tgatggccag gcttttgaat
     2161 ggcttcatgg ggggcggggt tcaggccaat acactcttgg cgaccgccac ggcgcccgca
     2221 gcgctagcgc tgtagccatc cgcgccgatc tggtcggcgt aggtttgcgt gatgggcgcc
     2281 cctcccacca aaacctttac ctggccgcga acgcccgcct ggttgagcgc gtccagcgtt
     2341 gtcttcattg ccggcatggt cgtggtccgg agcgccgaca tcgccacaat ctgggcgcct
     2401 ttttccttga cggccatgac aaattgctcc ggcgagacat tgacgcccag gtcaaacacc
     2461 tcaaacccgc cgccctcaag cagggcggtg acgaggttct tgccgatgtc gtggagatcg
     2521 cctttcaccg ttccgatgac gacgcggccg gtggatttcg ccccactggc gaccagcaac
     2581 ggccgcagca gttccagcgc tgccttcatg gcccgggcgg agagcagcaa ctcgggcaca
     2641 aaatattcgt tgctctcgaa acgccggccc acttccgcca tcgccggcat catgtagtct
     2701 tgcaccatct ttaacggttc gaccccgtcg gcaagggctt tctcggtgac cacccgggcg
     2761 gttttcgcat cgccgtttag aacggcatca tggagctgtt tgagttctga catatgattt
     2821 ctgatctatc gtgagctgtt tattctctcg cgcctcccca aaccactgga tacccgcaat
     2881 gagtccatgc ctatggaacg agcatcaggc ttcggtgatc acgcgcccaa agcgcgccat
     2941 cactggtgcg cggcaaggtg taaccgagct tgctgttttt gatgggctcg gcatgcccat
     3001 caaagtgagc cacgttcaac cggccggaat gccgcggcac cgtgcgggtt ggctcggcgg
     3061 agaaatcccc atccgtcggc acgcgaaaat agctgcaccc ggtgccaatt acgccaagca
     3121 aaacatccca ttcctgatct tcaacccagc ggtccggatt caaattttta ctcgcaaggg
     3181 taacccctcc ggcatcccca aaaaccacag acgcgcttgg cattgagacc tggctttccg
     3241 ccctcatttt cggactaacc gtggtggcag gaagcgtaac cgcgtattca gggtgattca
     3301 tgccaatctc cagggtgtta ttcgtgctaa ccgatcctcc gcgctccctc gccgcaagcc
     3361 agcgcatgga ggggcaatca aaaaccttgc gcgccttcgc atagcctccc agccgcaagg
     3421 catcctgcca aaagaggcca tatggatttt gcacgacgaa ggtgggagga tcgtaaatcc
     3481 agggatccca gcccggggcc ccaggctgcc gccagagcgg cagcagcttg cctgaattgt
     3541 catccaggta cagtttggtc gccaggccaa cctgcttcat attgttcagg caactgatgc
     3601 ttttggcttt ctccttggcc ttgcccaggg cgggcagaag caggctggca aggatggcta
     3661 taatcgctat gactaccagc aactcgatca gcgtgaagcc gccgccttgc gaccggtgcg
     3721 gagcggctcc agcttgtgaa tggcaaattc ttgctttcat cagtgctctc aaatcagcct
     3781 agggcgtcac cacgcgatag aaccgccggg cataattcgt ggattggagg tccgtaaaat
     3841 ccagcacccc attgataaag gtgccagttc ccacctggac ccagtcatta agcggttgag
     3901 ccacattggt gctggcaaag acccggtaag ccgcgttatc aggtcccgtg ccactaagct
     3961 gaaagttgcc atcatttaac ttcactatac caccgagata aggcgcgcta gaaacgactt
     4021 gaatcgtgcc gtccacggca aggttgttgg cccaggccaa gccaggatcg agcggcggaa
     4081 gactcaatga ggcaaaggtg ccgctccggg ctgccgcggc aaacagttgg aaggtatccc
     4141 cggcctccag cgcgtccggg ccaagattgg taacgaccaa gttgccgccg taaatcacgg
     4201 tggtcatgcc ttccactttg tcgttcgcgg taaccaagcc ggttttggca atctccatcc
     4261 gagccgttcc ctgcaaggtc agactgtcat tgatcgtcaa tgtggcgatg gatgctccgg
     4321 gggcaacggt gccgcctccc tcgatcacta ccggcccgcc aatcgcacca gagcccgaca
     4381 aggtgccgct agagcgaacc gtgacgctcc cgctcacggt gccagcgact cccagggttc
     4441 cctcaacgac atccagcgga atataggtcg aaacagtccc ccattcccag aagtctcccg
     4501 cgccacgttt cgttaacgtc aacaaattgg tgacgacggt atagaggctg ccgccgcgca
     4561 ccaacgtagc ccctggagcg acgttgatgg ttgcatttgt cggcataaag ccatgctgcg
     4621 ctatcgtcac cgtgccattg gtcacgcgca gcgcacccaa accatccggc cccgctccgg
     4681 ccaggtaaaa gtgttcatcg gtggaaatag tcccatcaaa aagcagcgct cctccgacat
     4741 caatcaccgt caaggtagtt ccgctgaagc cgccggggct aagcgcacta gagtccgtga
     4801 cgcggagtat gccgtcttga atttccgtca cgccataatt gttgcttgcc atgaggctga
     4861 cggtccccgg cccgactttt gtcaggttgc catttgccag atgggcaccg acaaccccgc
     4921 tggcgagggt gtagccggac gcagcaagca cgccattcgt ccccaggata ttgccatttg
     4981 ctaacgtcac ttgccccgcc gcctggttat gatcgcctat gttcaacgtg gcgccgttga
     5041 tagccaatgc gccccccgaa cctaaagcat tgggcgccgc cgcgatcact gagcccccgc
     5101 taattatatt ggctccgtaa tacgtgtttg cggtcatcat cgtgagctgc gcactgccct
     5161 tcttgatgag gcccatcggc ccgctgagac tgccaactcc gctaaagctg tagttcagag
     5221 agctgttgct cgccgtcata ctgtaagggg acaacgtggt tgtcaggttg actgaaccgc
     5281 tgtcggccgt gtcatcgaac cagaccggca cccccggcac ggatggctcc tcatacaccg
     5341 tgggggaacc attgccgatg ccaacccagt tgggcgtagc gctaatgtcc caatcgttgt
     5401 taacatttcc cttccatttc gggtaattta cttccgtaat caccaagtct atcacgctgt
     5461 gggcgacatc gttagacaac atggcggtaa cattgggaat tggcgccaac atcaacgatg
     5521 caaaaccgcc tccatcgagg ctgccgtact tgatgagcgg gaaaaccccg ggcaccggcg
     5581 atgccaggtt cgcgaccacg atatttgcga cgccaccggg ggttagcgct ccactagtct
     5641 ggataggcgc tacggtcgga aggccaagat tatttaaatc aatctccaac gtgctgtaca
     5701 tgaacaggcc aaagctgagg ctggcgtttg tcagcgaact gcctggatat gaaaccttaa
     5761 ccctgagcgt cgaagtgtcg gtcacggtat aatctccctt ggaaccgccg gctgtcgtaa
     5821 ccacgagtcg gcctccgtcg gccatagtcg gtccggtgta tgtgttggtg ccggtcaaat
     5881 aaacctcccc cgcgccaacc ttggtaaaac cgccatcggg agcgcccccc aaagcagggt
     5941 cgtgctcaag cgcttgcgga atggtcagcg tctgcccgcc actattcaaa atcagccccc
     6001 ctgacttgag ataggcatgg cttaagcctg aaatgaagtt gtcaaaccgg gcaggctgta
     6061 tgccccaggg agttatggtg ccgccatcga aggtagcaat ggcattgaca ttggccggcg
     6121 caactgcaaa gagttgatcc accgacagcg tgccgccgac cagattaaag gaactgtcag
     6181 ttccgggccg tacggtaatg gattgcgcgc tggcggtcgc cccgttgatc gtgagggtgc
     6241 ccggcgcggt gttgcccacg acaaattcca aaccgttgta gaaactgccc ccatccacca
     6301 acacgtttcc atcaatgaaa acgtagccgg cgatgtcatt gcccgccgca gtgagtgtca
     6361 gcgtgccgct cccggtcttg aacaagtgtc cgccgccgct aatcacacca ttgacaatta
     6421 ggctgtttcc tcccgtatcg agggttccgg aaacgccggg gtcgagcgaa atattcctgt
     6481 ttgcggacag cgccacgcta gctgcgccga cctgtagcgt gccccctccg ccagcaaatg
     6541 taagattggg ggtcggccag ccgggatcgg cccccagggc ggcatctgaa ttaatgttca
     6601 atgtcccgcc taaaatataa gagcctcccc catagctgtt tgcccccttc aagtacaagg
     6661 tgcctccccc gatcttacgc agggcggcat cacccgtcgc cccggccagc gtgccattga
     6721 tggtcatgtt ggcctggcaa tagatatccc gcacgtcagt agggccctgg tctaaagtga
     6781 ccgttccatt aaacgtctga tcattaccgg ccaggccgtc gattgtgcca ttgctcgcaa
     6841 tgtaacgaac cgtgcggatg atcacattgt tgttaatggc ccgcggccca ttcacggcat
     6901 acgaaaaaat ctggccctct ccggcagtgg caccaaaggc caccgtcccc gatcccagcg
     6961 ccgtatcgga gccaaaccca aggttagctc ccccaagaac tacggtgcct cccgcataac
     7021 tgttcccgcc ggtcagcaac gcatttaacc cggcggaatt ggccaccaac ccgcccgtgc
     7081 cacggatggt cccgtccagc cgtattgtgt tgcctgcaat tgcggtcagg tagatatcct
     7141 cgttgttggt tatggccccg ttgagtgtga gtttgagcgg cggagtaaga ttgttaacca
     7201 ggcggcggct gttagccacg cctccgctgg agttgacaag gacgttgttg tgcagggtca
     7261 aattacccga tccctgaaac gatagaactc cgaaatcggt cgagccggga accccgatgg
     7321 taaccgtgcc agtgcccaac ggccctgcgg tcggcgcacc gggcgaaccg gtggaactga
     7381 catcgcccac gcggacgttg ccggcgcgaa tataagcccc gccggaccaa gtggacaacg
     7441 ggttcgccat gagaaggaaa gccccggggg aaccgcccag cacattcgta aagctgattt
     7501 gatgagcccc agagatgcca acggtgtcgc caggagcatt ggtaaacaaa agggtccccg
     7561 tgccggaagc gcgccagatg ttgaggtcat gggccaggga aatcgggccg gcgattgtca
     7621 tgcccccgga ggaaagaatg tggttattaa ccgtgagatg gttcagggtg ttgacggcca
     7681 tgagcgacgc ggcggcgtcg gtagcattgc gcgtgatgct attaacgctg cgcacaacgt
     7741 ccacactgct ggtcaacgga tcgccggccg cattgatgaa gatcacatca tcaccattaa
     7801 ccggggcgac accgccaacc caattgccgg cagtggacca attggggtcg gggcttgcgc
     7861 ccccattcca ggtgcgcgaa gcggccgatg ccgacacggc aatgagcagc acgctaag
//
LOCUS       DAOPQG010000140         7420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_11087_length_7420_cov_14.822539, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000140 DAOPQG010000000
VERSION     DAOPQG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11087_length_7420_cov_14.822539"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKI07_12550"
     CDS             <1..227
                     /locus_tag="PKI07_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51915.1"
                     /translation="AAEAAQLAGRACRLSPCWGGAFPGAYAGAGRFAEAIQAVRRARE
                     RFAASGLNPLAEMAGRRLGLCPARQPSHQK"
     gene            288..2579
                     /locus_tag="PKI07_12555"
     CDS             288..2579
                     /locus_tag="PKI07_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC51916.1"
                     /translation="MTAARRKLAVGTGSLILLVVLLYLPSLSAGFIWDDDVMLTNNPL
                     VKAARGLYDIWFSTTLPDYFPLTSTTFWLEWRLWGMRPFGYHLTNVLLHALGAVLLWR
                     VLARLAVPGAWVAALVWAIHPVCVASVAWIAERKNTLSMVFFWLSILWYLRSEDRAPA
                     VAGPQPAARGSPARWYWLSLLAFLLALLSKTSVVALPLVLWLCAWWRHGNLSRRDLWR
                     SAPFFVLALVLGLVTVWFQTYRGLGTGMISSADPRLVRVLGGTWAVWHYLFKALLPLN
                     LMMIYPRWTIDPRSLLTYLPALVGFGLAGLFWVYRRSWGRPLLFALGYHVVTLAPVLG
                     FFDMGFLAYSRAADHWQYLALAGGVALVIGGGAHWLRRRFKGFPDSVADGGGEWLIIL
                     AVLLAVLTWRHQAVFASSERLWRETLARNPTAWVAHNSLGLILAGQGKLTEAVEHYTE
                     ALRLNPSDPASHYNFANALVEQGKLDEAAAHYRESLRLKPSNPGPHHNLAIVLNLQGK
                     YAEAAALCREALKLSPRDANIADTLGIVLFNDGKLSEAEQYCSAALAMDPHHANARDH
                     LGRLLARKGDLTNAILQFQAALQIQPDHAAARLDLGKALSAIGQFDQAAEHYGTALQL
                     QPAFAEAHYNWAILCLLQRQPNEALAHYREALRLWPDYADALNNLAWLLATSPEPKIR
                     DGREAVRLAEKACELAGRKQARFLGTLDAAYAESGRFAEAIATAKKAREQALAAGEKD
                     LAAAALQRQHEYEAGRPFREIVP"
     gene            2944..4503
                     /locus_tag="PKI07_12560"
     CDS             2944..4503
                     /locus_tag="PKI07_12560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOC51917.1"
                     /translation="MKRLIAFLSAGLFLAAVGVTSSYGQATSTSTWLGGSALFNVAGN
                     WSGGIIPNSSVLCVFSGSPPNTTITISAYSSHNNGLRFDAGAAAFTFVGTTTQAATEI
                     RTRGSGTGGLGQGIVNNSANQVTFNAPVRYYSFGAGTLLATEMCVINANGGNIAFNTA
                     TWGSANPALSGHGGTLQFIAASGKTITVGAATTPGIIATGGAGGGGLIKDGAGTLVLN
                     GTSANTYGGPTTVNAGTLTAGKVNALGAGTALNDLTINGGTLGIGNFNQAVDGVTVAG
                     GTITGGSGTLTGTSYALQSGTVNAKLGGSAAVTKTTAGTVTLGAANTYSGNTTISAGT
                     LKLGASGSFANSPNIIMNGGTLDVSAVSGFTLSASQTLKGNGNISGAVIANGTVSPGA
                     SIGTLTLDSSLTLGAAANTLMEINRAAVPQNADLISAASITLGGVLTVTDIGSGGFMA
                     GDSFNLFDGTLSGAFNSLILPVLPSGKTWDTSGLAAGGNGILSVVPEPSTAACFGLGV
                     AALLILRRRKA"
     gene            4990..5784
                     /locus_tag="PKI07_12565"
     CDS             4990..5784
                     /locus_tag="PKI07_12565"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOC51918.1"
                     /translation="MKIKIAILMAATLAFAASTQAQGNVLWQDDFESYANTAALSGPY
                     TQFYPSFPVLLDTTKGSAQSIHFDLPTANSQRRMWINIPGGPVTPTDQAPLKLEFWTD
                     LDTAIWSTRQYIEIRSYSDGEYNSGTLNQLIALGFTSSGVNTARINQRVMAGFTGTAW
                     GDLTDTYATRATIADPNNDWTKLAMVLYHNKVEFYVNDNLDTTQTMTAGVAFDSIVIG
                     SGLSSAGADVWFDGIKLTQVPEPSSAVLALLGGLGVFAWISRRRQA"
     gene            6110..6877
                     /locus_tag="PKI07_12570"
     CDS             6110..6877
                     /locus_tag="PKI07_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51919.1"
                     /translation="MLKTKREDWKLTMRIRNLVAIGAVSAMAFTASAATTYVNETFES
                     YADTAAMQVNWGSAGAGTLDTAFGNPGQSATHPGGAVNVWTGSSFSLLPSSGAIVLSA
                     DIYDPGTGPAQRNTVGLRNGANPLFEMGHYNSQGSQYAVRLVAMYGNPNWVFFPNVTR
                     TAGWHHYEATFTATDVTITLDLNNDGIVDSTLNYSGSPSANPFVDLRFGGPSGNSSAG
                     PMWVDNIMLSQIPEPSVAMLGLLGGLGMLWIARRRNV"
     gene            7255..7420
                     /locus_tag="PKI07_12575"
                     /pseudo
     CDS             7255..7420
                     /locus_tag="PKI07_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
BASE COUNT         1376 a         2192 c         2286 g         1566 t
ORIGIN      
        1 cagcggcgga agcagcccaa ttggcgggaa gggcctgccg gttgtcgccc tgctggggcg
       61 gagcgttccc aggcgcctat gccggagcgg gccgatttgc cgaggcgatc caggccgtgc
      121 gccgggcgcg cgaacgcttc gctgcttcgg gactcaatcc tttggcggag atggccgggc
      181 ggcgcctggg actctgcccg gcacgccagc cctcccacca aaagtaagcg gctgcgcaat
      241 ttggcatatc ttccagccaa agatccttta agatacgcgg caagtccatg actgcggcac
      301 gaagaaaatt agccgtgggc accggcagcc taatcctgct ggttgtcctg ctctacctcc
      361 cgtctctctc cgccggcttc atctgggatg acgacgtgat gctgacgaat aatccgttgg
      421 tcaaggcggc gcgcggcctg tacgacattt ggttttccac caccctgccc gattattttc
      481 cgcttacttc gacgaccttc tggctcgaat ggcggctttg gggaatgcgt cccttcggtt
      541 atcatctgac gaacgttctg ttgcacgccc tcggcgcggt gctgctctgg cgggtgctgg
      601 cgcgcctggc ggtgccggga gcctgggtgg cggccttggt ctgggccatc catccagtgt
      661 gtgtggcctc cgtggcctgg attgccgagc gcaagaatac gctctccatg gtcttcttct
      721 ggttaagcat cctgtggtat ttgcggtcgg aggaccgggc accggcggtt gccggcccgc
      781 aacccgcggc ccgtggctcg ccagcgcgct ggtactggct ttccctcctg gcctttctcc
      841 tggccctgct cagcaagact tccgtcgtcg cgttgcccct ggttctgtgg ttgtgcgcct
      901 ggtggcggca tggaaatctc tcgcgccggg acctgtggcg cagcgccccg tttttcgttc
      961 tagccctcgt cctggggctg gtcaccgtct ggttccagac ctatcgcggc ctcggaacag
     1021 ggatgatttc gagcgcagat ccccggctgg ttcgggtgct gggcggcact tgggcggtct
     1081 ggcattacct cttcaaagcc ctgctgccgc tgaacctgat gatgatctat cctcgctgga
     1141 ccatagatcc ccgttcgctc ctgacctatt tgccggccct tgtcggcttc ggcctggccg
     1201 ggttgttttg ggtctaccgc aggagctggg gccgccctct cctgtttgcc ctgggttacc
     1261 acgtggtgac gctggctccg gtgttgggat tttttgacat gggctttttg gcctattccc
     1321 gcgcggcgga ccattggcag tacctggccc tggccggggg ggtcgccctc gtcatcggcg
     1381 gcggggcgca ttggctgcgc cgccgcttca aagggtttcc ggattcggtc gcggacgggg
     1441 ggggcgaatg gttgataatt ctggcggttt tgctcgccgt tctgacgtgg cgccaccagg
     1501 ctgtttttgc cagttccgag cgtctctggc gcgagaccct tgcccggaac cccacggcct
     1561 gggtggctca caacagcctc ggcctgatcc tggcggggca gggaaagcta accgaagccg
     1621 tggagcacta caccgaagcg ctccggctca accccagcga tcccgccagc cattacaact
     1681 ttgccaatgc cctggtggaa cagggcaaac tggacgaggc ggctgcccat taccgggaat
     1741 cgctccggct taaacccagc aatcccggac cgcaccacaa cctggcgatt gtgctgaacc
     1801 tgcaaggcaa atatgccgaa gcggccgcgc tttgccggga ggcgctgaag ttaagcccgc
     1861 gcgatgccaa catcgccgac acgctgggca tcgtgctgtt caatgacggg aagctgtcgg
     1921 aagccgaaca gtactgttcg gctgccctgg cgatggatcc ccaccatgcc aatgcgcgcg
     1981 accacctggg ccgccttctg gcccgcaagg gcgacttgac caacgcgatc ctccaattcc
     2041 aggccgcgct gcaaatccag ccggatcacg ccgcggcccg cctcgacctg ggaaaggcct
     2101 tgagcgcgat cgggcagttt gatcaggctg ccgaacatta cggcaccgcg ctccagttgc
     2161 agcccgcgtt cgccgaggcc cactacaatt gggcgattct ctgcctgctg cagcggcagc
     2221 ccaacgaggc gctggcgcat taccgggaag cgctccggtt atggccggat tacgcggatg
     2281 cgctcaataa cctggcctgg ctcctggcca ccagcccgga gccgaaaatc cgtgatggac
     2341 gcgaggccgt tcgcctggcc gaaaaagcct gcgagcttgc cggacgcaag caagcgcgat
     2401 tcctgggcac gctcgacgcc gcttatgccg aaagtggccg gttcgccgag gcgattgcca
     2461 cggcgaaaaa agcccgggaa caggctcttg ccgccggaga aaaggacttg gctgcggcgg
     2521 ctttgcaacg ccagcacgaa tacgaagccg ggcggccttt tcgcgagatc gtcccctgac
     2581 ccttcagggc cctgaaaacg accgggctct cccgccaccc cgcccggggc tggaaatggc
     2641 gggctggaag caccgttcgc cagggtcgct ggactggaag ccgaaccccc ggcggcgcgg
     2701 gcaccggcaa ccgtttgtgg ggaaacgggc gtccggctaa tctatgcggg actgcgagct
     2761 ttggttgagt tgggctgggc gaaagcatga gttgtgccat ctgtaggcct tgacgccaga
     2821 aagcgtccgg aaaaataatt aaaaaagttg cgaaacgagt tgacgcgctc aagcgaattg
     2881 ctataacggt ctcgtcagat tgaatcgcgg tccaataaca atagaagtac agatagagat
     2941 tccatgaaga gactcattgc attcttgtcc gccggcctgt ttttggctgc ggtgggtgtc
     3001 accagttcct acggccaggc caccagcacg tcaacttggc tggggggctc cgccctgttt
     3061 aatgttgcgg gaaattggag cgggggcatt atccccaaca gcagtgtcct gtgcgtgttc
     3121 agtggcagtc cgcccaacac caccatcacc atttccgcgt acagcagtca taataatgga
     3181 ttgcgattcg acgcaggggc ggcggctttc acttttgtcg gcaccacaac ccaagccgcg
     3241 accgaaatac gcacgagggg ttccggcacc ggcggtttgg gccagggcat cgtgaacaat
     3301 agcgccaacc aggtgacgtt taacgcgccg gtcagatatt acagcttcgg cgccgggact
     3361 ctgctggcta ccgaaatgtg tgtcatcaac gccaacggtg gcaatatagc atttaataca
     3421 gccacgtggg gcagtgccaa ccccgctctc agtgggcacg gcggcacgct ccagtttatt
     3481 gcggccagcg gtaaaacaat taccgtgggt gccgccacaa cgcccgggat cattgcaacc
     3541 ggtggcgccg gtggcggcgg gttgatcaag gatggcgctg gcaccctggt tctgaacggg
     3601 acctccgcca atacttatgg cgggccgacc accgttaacg ccggaacgtt gacggcgggc
     3661 aaggtcaacg ccctgggggc ggggaccgct ctcaatgact tgaccatcaa cggcggcacg
     3721 ctgggcattg gcaacttcaa tcaggctgtg gatggggtga cggttgccgg cggcacgatt
     3781 accggtggct ccggcacttt gaccggaacc agctacgctc tccagagcgg cacggtcaat
     3841 gcaaagcttg gcgggtcagc cgccgtgacc aagaccacgg cgggcacggt gacgttgggc
     3901 gccgcgaaca cttatagtgg caacaccacc attagcgccg gcaccttgaa gcttggcgcc
     3961 tccggttcgt ttgccaacag cccgaacatc atcatgaatg ggggcacgct ggatgtttcc
     4021 gccgtcagcg ggttcacgct gagtgccagc cagacgctca agggcaacgg aaatatttcg
     4081 ggtgcggtga tcgccaacgg cacggtctcg ccgggagcct cgatcggcac gctcaccctg
     4141 gactccagcc tgacgctggg ggcagcggcc aataccctga tggagatcaa ccgcgctgcc
     4201 gttcctcaaa atgccgacct gatttcggct gcgagcatca ctttgggggg tgtgctgacc
     4261 gtcacggaca ttggcagtgg tggttttatg gccggcgatt ccttcaatct gtttgatggc
     4321 acgctgtccg gcgcctttaa ttccttgatc ctgccggtcc tgccgtccgg aaaaacctgg
     4381 gacacctcag ggctcgcggc cggcggcaac ggcattctgt cggtcgtgcc ggaaccctcc
     4441 acggctgcct gcttcggttt gggcgttgcg gccctgctga tcctgcggcg ccgcaaggct
     4501 tgagtttttc cctcaactgc cttttctctc atgcagcaag agccgccagt agtggcggct
     4561 cttgtgtttg tacgctgggt ccgggagcgg actttatcca accccaacga aaccccggca
     4621 tcctgggttg ttcaacgcct cgcttccaag ctccccctgc cgtccgtcac cctcttttgg
     4681 gagtcagcaa acttgccacc tgcatggatg cgcaaccgcg aggctgctgc gcgctggcgc
     4741 caagcccttg agggcaacgg ccagcgtaac ggttccgaaa cgggagggaa gggaaacatt
     4801 tttatctatc cggggctggg gatttttatg cgccgagcgg acggcccttt cccgcttgcc
     4861 gggggcggct tttgggggcg ccaaaagcgg gcaaaaaaaa attaagaaaa ttgttgacac
     4921 ggggtcagag atttggcaat ttagccgcac agataagatt aaacctgtat ctagagaaat
     4981 aaacgcatta tgaaaatcaa gatcgctatc ctcatggcag caacgctggc attcgcggcg
     5041 tccacccagg cgcaaggcaa tgtgttatgg caagatgact ttgagtcgta tgctaatacg
     5101 gcagccttaa gtggtcccta cacccaattc tatccgagct tcccggtact gttggatacg
     5161 actaaaggct ccgcccaatc catccatttt gacctgccga ctgcaaacag ccaacgtcgg
     5221 atgtggatca acattcccgg ggggcccgtg actccgactg atcaggcccc actcaagttg
     5281 gagttctgga ctgatcttga tacggcgatt tggagcacgc ggcaatacat cgagatccgt
     5341 agctactctg atggcgaata caactcgggc actcttaatc aactcattgc cttgggcttt
     5401 acttcctcgg gcgtgaatac tgcacggatc aaccagcgcg tcatggccgg atttactggc
     5461 actgcgtggg gcgaccttac ggatacttat gcgacccggg cgactatcgc ggatcctaac
     5521 aatgactgga ctaaattggc catggtgctc tatcacaaca aggttgagtt ctacgtcaat
     5581 gataacttgg atacgactca gacgatgacg gcgggggtcg cgtttgatag tattgtgatc
     5641 ggctcgggct tgagcagtgc cggggcagat gtctggtttg acggtattaa gctgacgcag
     5701 gttcccgagc cgtcctcggc ggtcttggcc ctcttgggcg ggttgggcgt ctttgcctgg
     5761 attagccgcc gtcgtcaggc ttaatcccgc gccttatttg tgattcggca agggccgcca
     5821 gtcatggcgg cccttgtgct tttcgaccag gaaacgggag cccctccttg ctgtttacgt
     5881 gccctgctct cgcaatcatc gagattatgg aacccgcacc cagggggggc tttcggcttc
     5941 tgcgtttggc gcccctgttt tggcagcccg tttacggtca tgaatgggtg agatgcgcca
     6001 aaaaaaatgt aaaaaaagaa gagaacagtg ttgacatgct cgcgggaact ggtataaaga
     6061 gatacgatta ggtgcttgtg tggcaagaag tgggcggcac aagcgcagaa tgctgaaaac
     6121 gaaaagagaa gactggaaac tgactatgag aatcagaaat cttgttgcga ttggagcagt
     6181 tagcgcgatg gcgttcaccg cgtcggccgc tactacttat gtaaatgaaa ccttcgagtc
     6241 ctatgctgac acggcagcaa tgcaggtgaa ttggggttcg gcgggagctg gaacactgga
     6301 taccgccttt gggaatccgg ggcaatcggc cacgcatccg ggcggtgccg ttaatgtttg
     6361 gacaggttct tccttctcgc ttctgccgag ttccggtgct atcgttctgt cggcggatat
     6421 ttatgacccc gggacagggc cggcgcagcg aaacactgtt gggctgcgca acggggcgaa
     6481 tccgctcttt gaaatgggcc attacaactc tcaaggttcc cagtatgcgg tccgtttggt
     6541 tgcaatgtat gggaatccta attgggtctt ctttcccaac gtgactcgga cggctggatg
     6601 gcatcactac gaggcgacct tcactgccac ggatgttacg atcactttgg atttgaataa
     6661 cgacggcatt gttgacagta cgttgaacta cagcggttcg ccttccgcaa atccgttcgt
     6721 tgatctgagg tttggtggcc catctggtaa tagctccgct ggtccgatgt gggttgacaa
     6781 catcatgctt tcgcagattc cggagccgtc cgtcgccatg cttggcctgc tgggcggatt
     6841 gggaatgttg tggattgccc ggcgccgcaa cgtgtaatca cctccagttt ttctgacgtg
     6901 ccaggggccg ccagcaatgg cggcccttgt gcttttcgac cagggaggac gagcgttccc
     6961 gcagaacgcg acttccccgt ccatctgcga gcgtcagggc agggacggct gctcgtccac
     7021 cctaaagaag ccgcggcatt ctcccgggcg cgtctggacc ctgcccccgg agcgccgact
     7081 tgcagtcggc tttgcgtgac cgaagaccgg tgagttccgc tttggccgat ggacattcta
     7141 agccgactgc aagtcggcgc tccggcctgg ggcgaatcgg ccacggtggc acgggcgaga
     7201 aggggcgcat tctcctttcc cgccgcggct ctttgcccta agctgacggc cacgatgcag
     7261 aaggagacca cgctggccct cttgcagcag atgctcggcc cggaggcgca attccgcgac
     7321 gggcaatggt aggccattga cttggcggcc aaccagcggc agcgattgct ggtcgtccag
     7381 cgcacgggct gggggaagag cgttgtgtat ttcctggcgg
//
LOCUS       DAOPQG010000141         7316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_11277_length_7316_cov_13.296240, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000141 DAOPQG010000000
VERSION     DAOPQG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11277_length_7316_cov_13.296240"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1060
                     /locus_tag="PKI07_12580"
     CDS             140..1060
                     /locus_tag="PKI07_12580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC51920.1"
                     /translation="METAMPSQPLSRRQFLERSAALTGALLLPACSLERPRAAARRTA
                     VDQVALGQTGLRLSRLGFGTGTNGGHVQFALGQEAFTSLVRYAYDQGITYFDCAQTYR
                     TFDWIKGALKGLPREKLFLQSKIPGKPDQVLAAIDRHRKVFDTDYVDSLLIHCMVKDG
                     WTDGWKRIMDAFDEAQAKQWIRAKGVSCHSLPALRTAAASPWPQVHLVRVNPQASHID
                     GLDENWNGPNRDVALVLQELAVMRAKGRGVIGMKLIGNGDFTDPADREKSIRFAMRRP
                     ELDAVVIGFKNRAEIDEAIQRMNQALAEAA"
     gene            1154..1309
                     /locus_tag="PKI07_12585"
     CDS             1154..1309
                     /locus_tag="PKI07_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51921.1"
                     /translation="MVPSRSRHKRRRHSRRKRQLIFLVGVLALAAVALVAVIMYGLTS
                     MNWRARY"
     gene            1337..2020
                     /locus_tag="PKI07_12590"
     CDS             1337..2020
                     /locus_tag="PKI07_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOC51922.1"
                     /translation="MRCVVTAGPTFEPLDAVRRLTNFSTGRLGSELADFLAARGHDVT
                     LLRGEQATWRGEVRARRVQTFTTTADLRARLQALAGPAVAAVFHAAAVSDYTFGRVWN
                     PRRAGRPAKPGRGKLASRHGPLLARLVPTPKIIADLRAWFPRARLVGWKYEVEGGRAR
                     ALRRAARQLAEYHTDGCVANGPAYGAGFGLVRADGSCLHLADAPALFAALEAFLRPPP
                     AAEPGGAPD"
     gene            2078..4072
                     /gene="recG"
                     /locus_tag="PKI07_12595"
     CDS             2078..4072
                     /gene="recG"
                     /locus_tag="PKI07_12595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOC51923.1"
                     /translation="MTAATANEPLPPLGQAVTILPGVGPERAAQLARLEIRTLEDLLR
                     HRPARHEDRRHFSPIAGLQLGVPATTRGRVIAHGVKWYQHHTRSIFELILDDGTARLH
                     CRWWNLPFMEKYFAVGQEVIAFGKPVNLKPRTLDHPETEVCENGEEDFIHFNRLTPIY
                     PLTEGLPQRWLRALIWRTLGQFADAIRASGPARALDAAVPALPPRAQAIRWLHFPDDP
                     AQPELARRRLALEEFLALQRRIQLRRQNFTARARARPCAGDNRLIKPFLARLGFTLTG
                     AQTRVLRELRRDMSGAHPMRRLLQGDVGSGKTVVAACCGLMALESGYGVALMAPTEVL
                     AEQHFANFTRWLAPLGVPVELRTGSRKTGAGSPPRAPGLTLGTHALLTAGFALPGLGL
                     VIIDEQHKFGVAQREQLVRKGAYPHLLVMTATPIPRTLGLTLYGDLDISVLDELPPGR
                     GRLQTFVRAPDKRPQVWDFIRGKLAAGRQAYIVYPRVEDAGPGGLKAVTREYAALRQT
                     LAPHRVGLLHGRLPPREKEAVMAAFRARQIHALLATSLIEVGVDVPNATVMLIENAEQ
                     FGLAQLHQLRGRIGRGAHDSWCILVAAAQHPEARARLQVLAETTDGFRIAEADLRLRG
                     PGELLGQQQSGLPRFRFGDLAADFDLIQQARELAARQPEA"
     assembly_gap    4084..4183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4191..5954)
                     /gene="polX"
                     /locus_tag="PKI07_12600"
     CDS             complement(4191..5954)
                     /gene="polX"
                     /locus_tag="PKI07_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOC51924.1"
                     /translation="MNKDQVAEVLQEIGTLLELQGENPFKTRAYLNAARTIGRLEEPL
                     EAVIAAGRLGALPGIGAALRDRITELAATGRLAYYEELKAAVPAGLRALLEIPGLGPR
                     KIKALRDALGIESVAQLEQACREGRVAGLAGFGAKTQANLLAGIARRQSYASYFRLGP
                     ALAAAEPLQAALRAHPDVIRCDTAGSLRRCREVIGDIDLLASSARPARVIADFIRQPG
                     VARVLARGETKASVLLESGMQADLRVVRDAEFPAALLYFTGSKEHNVVLRQRALERGL
                     RLNEYGLFRSRAETRDPRRRLPCAAEAEIFARLGLRYIPPELREDRGEIPAAEQGPLP
                     RLVEWTDLQGSLHNHSNWSDGQQRLEEIARLGRELGLAYWAVTDHSKSSVQAHGLEAR
                     RVRQQTREIAALNEQLAREGAGFRLLTGIEVDILKDGALDLPDDLLAELDVVVASVHQ
                     SLGQPEAEMTRRLIRAAQNPCVHILGHLTGRLLLEREPSAVDARAVIDACAETGTWIE
                     LNAHPQRLDLDWRHWPYAKSRGVKCAINCDAHRAEHAGFLRLGAGIARKGWLTREDVV
                     NTLPLDALRQELGRKRARAGL"
     gene            complement(5985..7265)
                     /locus_tag="PKI07_12605"
     CDS             complement(5985..7265)
                     /locus_tag="PKI07_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOC51925.1"
                     /translation="MSSGTVFALGVGLLLAAWWAARVGLEQLNRRHALAHADAAPEEF
                     RHVMDAATYARSVQYTLAQGRFEQVAATVDAVARLGALFSPALPWGYRAWTAGLGESA
                     WSVGAFLLAAGGALSLFGLPLDWYHQFRLEQRFGFNTTTPRLWWADRARGLLLAAALG
                     YPLVVLVLGLMAWAGAGWWGWAWGAWLGFQVLLQGLAPVLILPLFNRFTPLPAGPLRE
                     RLLRLAARTRFRARAIQVMDGSKRSRHSNAFFTGFGRFRKIVLYDTLVEQLTAPELEA
                     VLAHEIGHYRMRHLPKLLLGSAAGALAGFWLLAWLAGRAWFYEGFGFAPGYPGPALLL
                     FGLLAGVAGFWLAPLWRGWSRRCEYQADAFAAAAVGGPEPLLGALRQLAEKNLSNLTP
                     HPCYRRFYYSHPTLRERERALAARSASAAAGFGL"
BASE COUNT         1094 a         2740 c         2376 g         1006 t
ORIGIN      
        1 ctctggcccg cttagcccgt ttggaggctg taagcgggca agatgcccgc gctccggcct
       61 tgcaggtgtg aaatgtctgg gctagagtaa aggccgggat ggcggcgcgc cgcgcggttg
      121 tcccgcgcaa ccttgattta tggagaccgc tatgccgagc cagccattaa gccgccgcca
      181 gtttctggaa cgttccgccg ccctgaccgg cgcgctgctg ttgcccgcgt gttccctgga
      241 gcggccccgg gcggcggccc ggcgcacggc ggtggatcaa gtggccctgg gccagaccgg
      301 cctccggctc agccggttgg gcttcggcac cggcaccaac ggcggccatg ttcagttcgc
      361 cctggggcag gaggcgttca cctcgctggt gcgctacgcc tacgaccagg gcatcaccta
      421 ctttgattgc gcccagacct accgcacctt tgactggatc aagggcgccc tcaaggggct
      481 gccccgcgag aagctgtttc tccagtccaa aatccccggc aaacccgacc aggtgctcgc
      541 cgccattgac cggcaccgca aggtttttga caccgactac gtggacagcc tgctgatcca
      601 ttgcatggtc aaggacggct ggacggatgg atggaaacgc atcatggacg cctttgacga
      661 ggcgcaggcg aagcagtgga ttcgggccaa gggcgtctcc tgccacagcc tgcccgcctt
      721 gcgcaccgcc gccgcctccc cctggcccca ggtccacctc gtgcgcgtga atccacaggc
      781 cagccacatt gacggcctgg acgaaaattg gaacgggccg aaccgcgacg tggcgctggt
      841 gctgcaagag ctcgcggtca tgcgcgccaa aggccgcggc gtcatcggca tgaagctcat
      901 cggcaatggc gacttcaccg atcccgccga ccgggagaag tccatccgct ttgcgatgcg
      961 ccggccggag ctggacgcgg tcgtgattgg cttcaagaac cgggcggaaa ttgacgaggc
     1021 catccagcgc atgaaccagg cgctggcgga ggcggcatga gccgcgccgg gccgttcccg
     1081 gctcccccgc gcgcccgcgc cggctcgcga aaaaaggctt tacccggccg cggcggatgc
     1141 ctatcattcc tgcatggttc cttcccggag ccggcacaaa agacggcgcc attcccggcg
     1201 caagcggcag ctcatttttc tggtgggggt gctggcgctg gcggcggtgg cgctggtggc
     1261 cgtcatcatg tatgggttga ccagcatgaa ctggcgcgcc cgctactaac ccgcggcgcg
     1321 ggcggattcc cggcttatgc gatgcgttgt cacggctggc cccacgtttg aaccgctgga
     1381 tgcggtgcgg cggctgacca acttttccac cggccggctg ggctcggagc tggccgattt
     1441 cctcgcggcg cgcgggcacg acgtgacgct gctgcggggc gagcaggcca cgtggcgcgg
     1501 ggaggtccgc gcccggcgcg tgcagacgtt taccaccacc gccgacctgc gcgcccgctt
     1561 gcaggccctg gccggcccgg cggtggcggc ggtgtttcac gcggcggcgg tgagcgatta
     1621 cacgtttggc cgggtctgga atccgcgccg ggccggccgg ccggcgaaac ccggccgggg
     1681 caagctcgca agccgccacg ggccgttgct ggcccggctg gtgccgacgc cgaaaatcat
     1741 cgccgacctg cgcgcctggt ttccccgggc gcggctggtg ggctggaagt atgaagtcga
     1801 gggcggccgc gcccgcgcgc tccgccgggc cgcgcggcaa ctggccgaat accacacgga
     1861 tggctgcgtc gccaatggcc cggcttacgg cgcgggcttc gggctggtgc gggcggacgg
     1921 atcgtgcctc cacctggccg atgcgccggc gctcttcgcg gcgctggagg ccttcctgcg
     1981 cccgccgccc gccgcggagc cggggggcgc cccggactga gccgcccggg atttcaggct
     2041 tctcggtttg cgcggcgtcc tgctatggtg cgccgggatg accgctgcga ccgccaacga
     2101 accgcttccg cccctgggcc aggcggtcac gattttgccc ggcgtcggcc cggaacgcgc
     2161 cgcgcaactg gcccggctgg agattcgcac cctcgaggac ttgctgcggc atcggccggc
     2221 ccggcacgaa gaccgccgcc acttttcccc catcgccggg ctgcaactgg gcgtgccggc
     2281 caccacgcgg ggccgggtga tcgcccacgg agtcaagtgg tatcaacacc acacccgctc
     2341 catcttcgag ctgatcctcg atgacggcac cgcgcggctg cattgccgct ggtggaacct
     2401 gccgttcatg gagaaatact tcgcggtggg ccaggaagtg attgcgttcg gcaaaccggt
     2461 gaacctcaag ccccgaaccc tcgaccaccc ggagaccgaa gtctgcgaaa acggcgagga
     2521 ggacttcatc cactttaacc gcctcacccc catctacccg ctgaccgagg ggctgccgca
     2581 gcgctggctg cgcgcgctga tctggcggac gctcgggcaa ttcgcggacg cgattcgcgc
     2641 gtccgggccg gcgcgggcgc tggacgccgc agtccccgcg ctgcccccgc gcgcccaggc
     2701 catccgctgg ctgcacttcc cggacgaccc ggcgcagccg gaactggccc ggcggcggct
     2761 ggcgctcgag gagttcctgg cgctgcagcg gcggattcag cttcggcggc aaaacttcac
     2821 cgcccgggcc cgcgcccggc cgtgcgccgg cgataaccgg ctcatcaaac cctttctggc
     2881 gcggctgggc ttcacgctga ccggcgcgca gacccgcgtc ctgcgcgagc tgcgccgcga
     2941 tatgtccggc gcccacccca tgcggcggct gctccagggc gacgtcggct ccggcaaaac
     3001 cgtcgtcgcg gcctgctgcg gcctgatggc gctggaaagc ggctatggcg tcgcgctcat
     3061 ggcccccacc gaggtcctgg cggagcagca cttcgcgaat ttcacccgct ggctggcgcc
     3121 gctgggcgtg ccggtggaac tccgaaccgg cagccgcaaa acgggcgccg gaagcccccc
     3181 gcgcgcgccg gggctgaccc tcggcacgca cgcgctgctg acggcgggct ttgccctgcc
     3241 cggcctgggg ctggtgatca ttgacgagca gcataagttc ggcgtcgcgc agcgcgagca
     3301 gttggtgcgc aaaggcgcct acccgcacct gctggtgatg accgccaccc ccatcccccg
     3361 cacgctgggc ctgacgctct acggcgacct cgacatctcc gtccttgacg aattgccccc
     3421 cgggcgcgga cggctccaga cgttcgtgcg cgcgccggac aagcggccgc aggtctggga
     3481 tttcatccgc ggcaagctgg ccgccgggcg gcaggcgtat attgtttacc cgcgcgtgga
     3541 agacgccggc cccggcggcc tcaaggccgt cacccgcgaa tacgcggcgc tgcggcagac
     3601 gctggcgccc caccgggtag gcctgctgca cggacggctg ccgccgcggg aaaaggaggc
     3661 ggtcatggcc gcctttcgcg cgcggcagat tcacgcgctg ctggccacgt cgctcatcga
     3721 ggtcggcgtg gacgtgccca acgccacggt catgctcatc gagaacgccg agcagttcgg
     3781 cctggcgcaa ctgcaccaac tgcgcggccg gatcgggcgg ggcgcgcacg attcctggtg
     3841 catcctcgtc gccgcggccc aacatcccga ggcgcgcgcg cgcctgcagg tgctggcgga
     3901 aacgacggac ggcttccgca ttgccgaggc cgacctccgc ctgcgcggcc ccggcgaatt
     3961 gctcggccag cagcaaagcg gcctgccccg cttccgcttc ggcgacctcg ccgccgactt
     4021 cgacctcatc cagcaagccc gtgaactggc ggcgcgccag cccgaggcct gatccacgcc
     4081 ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggagcgc tcagaggccc
     4201 gcccgggctc gtttgcggcc caattcctgg cggagggcgt cgagcggcag cgtattgacc
     4261 acgtcctcgc gggtgagcca gcctttgcgg gcgatgcccg cgcccaggcg caggaaaccg
     4321 gcgtgctcgg cccggtgggc gtcgcagtta atcgcgcatt tgacgccccg ggatttggcg
     4381 tagggccaat gccgccaatc caggtcgagc cgctgcgggt gggcgttcaa ttcaatccag
     4441 gtgccggttt ccgcgcaggc gtcaatcacc gcccgcgcgt ccaccgcgct cggctcgcgc
     4501 tcgagcagca gccgcccggt caggtgcccc aggatatgga cgcacgggtt ttgcgcggcg
     4561 cggatgagcc ggcgcgtcat ctccgcctcc ggctgcccga ggctttgatg cacgctggcg
     4621 accaccacgt ccagctccgc cagcaggtca tcgggcaaat ccagcgcgcc gtccttgagg
     4681 atatccacct cgatgccggt cagcaggcga aagccggcgc cttcccgcgc gagctgctcg
     4741 ttgagggcgg caatttcccg cgtctgctgg cgcacgcgcc gggcctccag cccgtgggcc
     4801 tggacggagg acttcgagtg gtcggtgacc gcccagtagg ccagccccag ctcgcgcccc
     4861 agccgggcaa tctcctccag ccgctgctgg ccgtcgctcc agttcgagtg attatgcagc
     4921 gagccttgca aatccgtcca ctcaaccagc cgcggcagcg gcccctgctc cgccgccggg
     4981 atttcccccc ggtcctcgcg cagctccggc ggaatgtagc gcaggcccag gcgcgcaaag
     5041 atctccgcct ccgccgcgca cggcagccgc cgccgcggat cgcgggtctc ggcgcgcgag
     5101 cggaagaggc cgtattcatt cagccgcagg ccgcgctcca gggcgcgctg gcgcaggacc
     5161 acattatgct ccttgctgcc ggtgaaatac aacagcgccg cgggaaactc cgcgtcgcgc
     5221 accacgcgca gatcggcctg catcccgctc tccagcagca cgctggcctt ggtctcgccc
     5281 cgggccagca cccgggccac ccccggctgg cggatgaaat cggcaatgac ccgcgccggc
     5341 cgcgccgacg aggccagcaa atcaatgtcc ccgataacct cccggcagcg ccgcaggctc
     5401 cccgccgtgt cgcagcggat cacgtccgga tgcgcccgca gcgccgcctg caagggttcg
     5461 gcggccgcca gcgccgggcc gagccggaag tacgacgcgt aggactggcg ccgggcgatg
     5521 cccgccagca ggttcgcctg cgtcttggcc ccaaaaccgg ccagccccgc cacccgcccc
     5581 tcgcggcagg cctgctccaa ctgcgccacc gattctatcc ccagcgcgtc gcgcagggcc
     5641 ttgatcttcc gcgggcccag ccccgggatt tccagcaacg cccgcagccc ggcgggcacc
     5701 gcggctttca gctcctcgta atacgccagc cgcccggtgg cggccagctc ggtaatccga
     5761 tcccgcagcg ccgcgccgat gcccggcagc gcgcccagcc gccccgcggc aatcaccgcc
     5821 tccagcggct cctccagccg cccgatcgtg cgggccgcgt tcaggtaggc gcgcgtcttg
     5881 aacgggttct cgccttgcag ctccagcagc gtcccaatct cttgcagcac ttccgcgacc
     5941 tggtccttgt tcataacctc aatatggcct ttccccccgg ggcgtcaaag cccaaaaccc
     6001 gccgccgcgg acgcgctccg ggccgccagc gcccgctcgc gctcgcgcag cgtgggatgc
     6061 gagtaataga aacggcggta gcacgggtgc ggggtgaggt tgctgagatt cttttcggca
     6121 agctggcgca gcgccccgag cagcggctcc ggcccgccca ccgccgcggc ggcaaaggcg
     6181 tcggcctggt actcgcagcg gcgcgaccac ccgcgccaca acggcgccag ccagaagccg
     6241 gccaccccgg ccagcagccc gaaaagcagc agcgccggcc cgggataccc cggggcgaaa
     6301 ccgaagccct cgtagaacca ggcccgcccg gccagccacg ccagcagcca gaagcccgcc
     6361 agcgcgcccg cggcggagcc gagcagcagc ttcggcaaat gccgcatccg gtagtgcccg
     6421 atctcatgcg ccagcaccgc ctccagctcc ggcgccgtca gttgctccac gagcgtgtcg
     6481 tagagcacaa ttttgcggaa ccgcccgaag cccgtgaaga acgcgttgga atgacgcgac
     6541 cgcttgctgc cgtccatcac ctggatggcg cgggcgcgga accgcgtgcg cgcggccagg
     6601 cgcagcagcc gctcccgcaa cgggcccgcg ggcaggggcg tgaaccggtt gaagagcggc
     6661 agaatcaata ccggcgccag cccttgcagc agcacttgaa accccagcca cgccccccag
     6721 gcccagcccc accagcccgc ccccgcccac gccatcagcc cgagcaccag caccaccagc
     6781 ggataaccca gcgccgccgc cagcagcagc ccccgcgccc ggtccgccca ccacaagcgc
     6841 ggcgtcgtgg tgttgaagcc aaagcgctgc tccagccgga actggtgata ccaatccagc
     6901 ggcagcccga acagcgacag cgccccgccc gccgccagca aaaacgcccc caccgaccac
     6961 gctgattccc ccagccccgc cgtccaggcc cggtaacccc acggcagcgc cgggctgaac
     7021 agcgccccca gccgcgccac cgcgtccacc gtggccgcca cctgctcgaa ccgaccctgg
     7081 gccagcgtgt actgaaccga ccgggcgtag gtcgccgcgt ccatgacgtg ccggaactcc
     7141 tccggcgccg catccgcatg ggccagcgca tggcgccggt tcaactgctc caaccccacc
     7201 cgggccgccc accacgccgc cagcaacagc cccacgccca acgcaaatac cgtgcccgaa
     7261 ctcatgcccg cagtctaacc aatccccgcc gccgccgcca cgacgaagtg gcctcc
//
LOCUS       DAOPQG010000142         7232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_11448_length_7232_cov_15.746551, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000142 DAOPQG010000000
VERSION     DAOPQG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11448_length_7232_cov_15.746551"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..866
                     /locus_tag="PKI07_12610"
     CDS             111..866
                     /locus_tag="PKI07_12610"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOC51926.1"
                     /translation="MKAIRLLVVAAAAAWACLLPEMAAGAAQKSPDKIRLLVITGGHG
                     FQKDSFFQLFADNPDIAYQAVEHPNAHARLTAEAAKQYDVIVTYDYNQAISEEAKANF
                     LARLKEGKGLVALHHAIAAYPNWPEYWNIIGARYYLAATNINGVAKPRSKARDDMTFR
                     IHVADPRHPVTRGIKDFMVRDETYKWFDVAPKCHPLLTTDEPENNEVLSWAKTYRRAR
                     VVYLQLGHDRQTYENPNYQQLLRQAIRWVARRH"
     gene            967..1752
                     /locus_tag="PKI07_12615"
     CDS             967..1752
                     /locus_tag="PKI07_12615"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOC51927.1"
                     /translation="MFFHVIACEIAFRELCHAASQSQNLVDFDFLTQGYHDIPGAGRA
                     EIQKRIDAVPAGRYDAILLGYGLCSSILPGLTTAHTPLVIPRAHDCITFFLGSKERYR
                     KCFDARPGTYYYTSGWLECTTRRRGTGATWDGASLPAGTKDSIKATYYEEWVKKYGES
                     QADYLLTEMNRWADSYTHGTLIHFDLVQHLKLNEQVRQICAERGWQYDEIPGDLRLLH
                     QLLAGEWPESDFLIVSPGQTVIATNDERIIGVAPASSNGPTAA"
     gene            2139..2459
                     /locus_tag="PKI07_12620"
     CDS             2139..2459
                     /locus_tag="PKI07_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51928.1"
                     /translation="MRILIIACLALLAVGCQTSRTSVNPIAYQGGDGSSYEQAILIRE
                     AHCREAGILAQRLWLERTYPGYRAAKESTQTLANRQYAVFEIATAEGQTRTVYFDTTE
                     FANK"
     gene            complement(2593..2943)
                     /locus_tag="PKI07_12625"
     CDS             complement(2593..2943)
                     /locus_tag="PKI07_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51929.1"
                     /translation="MNNALETFLSSRLPIAGLVAYSIHLPDRVLEVQCLSKSFYPSAA
                     EDMLTGLVYAGQTLLPANQQAAHYCWVFDCIRVYVAARSDGTSLALLVENNPGAEMDR
                     IKDTLTAFIEQPSS"
     gene            complement(2940..3971)
                     /locus_tag="PKI07_12630"
     CDS             complement(2940..3971)
                     /locus_tag="PKI07_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC51930.1"
                     /translation="MIIDDDPQFLAAIGASFKELSGGRWAIRTATNHSQALEHLKAHP
                     MNLVVLDVEMPVMDGVEFLRLLGRTHPGQQVVMLSERMEETVRQTCLDLGAILFLAKP
                     SSPEDFHNLFITLDSLATARSASGFRGVMQQVGLDDVLQMECLARKSSILKVTTGKRQ
                     GQIYISEGEIIHAQTGQLEGEMALYGLLALKGGEFKLQPFAEPARRTISGQYQFLLME
                     AARLRDEGELQPGRGFGGLGRPADFGATSFDASAPTEARGTQIEEVLLCSNAREVLYE
                     SKCESIENRLELFAKLEQQANQAAKGTPSGRFHRMWMSTGSSRMLVQIQPAFKLLVRS
                     ALPPASDAE"
     gene            complement(4011..5303)
                     /locus_tag="PKI07_12635"
     CDS             complement(4011..5303)
                     /locus_tag="PKI07_12635"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC51931.1"
                     /translation="MSALATAALTDSDVEVRRIAATALGRSEHPGRYEPLLKALEDKD
                     PEVIRAALFGLRKIVDERVLVELVRLLRHPNFHVRVGAAQAIDTVRWTPKDREERIWF
                     QNAKGWYRRAAAAGVDALPALQLTIQTGPVQAAAGAVEALGQISDPRIIKLLRSALQA
                     EEPTVRVAAVDALGKVGGGEAVDALTTSLRNSHPQVRVAAAQALGVLRAAEAIGPICK
                     LLRDKEWEVRQEAAIALGRFNNPAACEPLASALNDTDADVREAAAMALGRIGDRRGVA
                     PLILALKDEVTSVRRIAAASLARIDPDWASLPETRGVAEQLKLAIQDAEPAIRFFVAQ
                     LLVNLGEMAADHTFEFTPEDHLGSPAIKRKRMATHLFIGMLEDPDRDVRQAAAEALGR
                     LGGERARQALARTAADVDGDVAAAIQMALQALGTESGS"
     gene            complement(5622..6650)
                     /locus_tag="PKI07_12640"
     CDS             complement(5622..6650)
                     /locus_tag="PKI07_12640"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC51932.1"
                     /translation="MNTNLGGILAGVLMASTLFAQQATDPYMGDWQGEISVNDESQKV
                     GVYMIPLGGGKYEARLVSDFLQRGPYLYRLKGEIRDGQVRFMDDIPFDISRVFGTTGN
                     GVVLSAALWSGAVEGGNARGTMAGRLRGTFEWKQTQRISPELGKAPPAGAIVLFDGKN
                     LDQWRHREEGKPVKWKVLPDGVMEVNGGDIMSREKFLDQRVHLEFRLPYMPTAFGQGR
                     ANSGVYLQGRYEVQVLDSYGLEGQDNECGGVYTVSRPRVNMCAPPLQWQSYDITFTAA
                     KFDAAGKKTARARITVVHNGVVVQDNLELPGVTGGALNDRENEPGGLLLQDHGNPVQY
                     RNIWVEKR"
BASE COUNT         1479 a         2415 c         1931 g         1407 t
ORIGIN      
        1 caccttgagt ggaaaccatg ataaccaatt tgctgaactt gacggacaca actcgttttg
       61 ccgacagatt gcttttgaaa ccggggcgca tttgtgctac aaaagggcct atgaaagcga
      121 tacgtttgct ggtggttgcc gcagccgcag cctgggcatg cctgctgccg gagatggcgg
      181 cgggagcggc gcagaagtct ccggataaga ttcgcctgct ggtgatcacc ggcggacacg
      241 gattccagaa ggactccttt ttccagctct tcgcggataa ccccgacatc gcttatcagg
      301 cggtggagca cccgaacgcg catgcgcggc tgacggccga ggcggcgaag cagtacgacg
      361 tgatcgtcac ttacgactac aaccaagcga tctcggaaga agcgaaggcg aatttcctgg
      421 cgcggctcaa ggaggggaaa ggcctggtgg ctctgcacca cgccatcgcc gcttacccga
      481 actggccgga gtattggaac atcatcggcg cgcgctacta cctggcggca acaaacatta
      541 acggcgtagc caaaccgcgc tcgaaggccc gggatgatat gacttttcgg attcacgtgg
      601 ccgacccgcg ccacccggtg acccgcggga tcaaggactt catggttcgc gatgaaacct
      661 ataaatggtt cgacgtcgcc ccaaaatgcc atccactgtt gaccaccgac gaaccggaga
      721 acaatgaagt cctctcatgg gccaaaacct accggcgcgc ccgggtggtt tatctccagc
      781 ttggccacga tcgccagacc tacgaaaacc ccaattacca gcaactgctc cggcaagcca
      841 tccgctgggt ggcgcgccgg cattgaactg cccgggctgc gcatggaaca accgggttaa
      901 atcggaacct gctcgcattc gccccggcag cttcgcgcct ttatcctgtc agccgccgcc
      961 tccctcatgt tcttccacgt catcgcctgc gagattgcct ttcgcgagct ctgccatgcc
     1021 gcatcccagt cgcagaacct ggttgacttc gacttcctga cccagggcta tcatgatatt
     1081 cccggggcgg ggcgcgcgga aatccagaag cgcattgatg ccgtgcccgc cggcaggtac
     1141 gacgcaatcc tgcttggcta cgggttatgc agcagcatcc tccccgggtt gacgacggcg
     1201 cacacgccgc tggtgattcc ccgcgcgcac gattgcatca cgttcttcct gggctcaaag
     1261 gaacgctacc gcaaatgctt cgacgcccgg cccggcacct actactacac ctccggctgg
     1321 ctggaatgca ccacccgccg ccggggaacg ggggccacct gggacggcgc ttccctgccc
     1381 gccggcacca aggacagcat caaggcaacc tattatgagg aatgggtgaa gaaatacggc
     1441 gagagccagg ctgattacct gctcacggaa atgaatcgct gggcggattc ctatacgcat
     1501 ggcaccttga tccactttga tttagtccaa cacctgaagt taaatgagca ggtgcggcag
     1561 atctgcgcgg agcgaggctg gcaatacgac gaaatcccgg gagacctccg gttgctccat
     1621 caactgctcg cgggcgaatg gccggaatcg gattttctca tcgtgtcccc ggggcaaacg
     1681 gtgattgcca ccaacgacga gcggatcatc ggcgttgcgc cggcatcttc aaacggtccc
     1741 actgccgcct agcctctcgg ctttgtggct cccgcctccg ggttgcggcc agcgtagaaa
     1801 gcagcgaccc ttttccccta tggcgttacg cggctgaagc aggctccccg ggcgctgtgc
     1861 gcgcgcaaac aactgggtct tataaatacc cggggccgcc gccggaggct ggcaaacctg
     1921 ttcggacttc ccgagaacag tccgcggaat cctgatcgtc aaattatcag ttttgcgaaa
     1981 ttacacgccg gagaggcgga aaaaaccggc gttcgcgggg ttgaacctct ctggcagagg
     2041 tgtttttggc aaatttagcc gcccggattc gcaattttgc gccagaaaac gcgtggcaca
     2101 cgttgtgccc tgctcaaact gagaaagcaa cgctggttat gagaatactg ataatagcat
     2161 gtctcgccct cctggcagtc ggctgccaga cttcgcgaac ctccgtcaac ccgatagctt
     2221 accagggcgg cgatggttct tcgtacgagc aggccatcct cattcgtgaa gcgcactgcc
     2281 gggaagcagg cattctggca caaagattgt ggctggagcg aacttatcca ggatatcgcg
     2341 cggcgaagga gtcaacgcaa accctggcca accggcaata tgccgtgttt gagattgcca
     2401 cggcggaagg tcagacgcga accgtgtatt ttgacaccac cgagtttgcc aacaaatgat
     2461 gggcatcccc gaagggcgcg cggagcaccg ctgaactcca gcacgcctgc caatgctgac
     2521 agttgattaa tcgcttctcc gtcgcatcgt gcggacgtca ttcccacccg gaatctccct
     2581 tggtcgacag gctcaagacg atggctgctc aataaaggca gtcagggtat ctttgattcg
     2641 atccatctct gctccgggat tattttcgac cagcagcgcg aggctggttc catccgatcg
     2701 agctgcaacg taaacccgaa tacaatcaaa gacccagcag tagtgggccg cttgctggtt
     2761 ggcaggtaaa agggtttgcc cggcataaac caggcccgtc agcatatcct ccgccgccga
     2821 gggataaaaa gacttgctca ggcattggac ctcgagcaca cgatccggca ggtggatgct
     2881 atacgcgacc aggccggcta tcggcaggcg cgacgaaaga aaggtctcca gtgcgttatt
     2941 cattcggcgt cggaggcggg tggcaacgcg ctgcgcacca atagtttgaa ggcaggctgg
     3001 atttgaacca gcatccggct gctgccggtg ctcatccaca tccggtggaa tcgtccgctg
     3061 ggcgtgcctt tggccgcctg gttcgcctgt tgctcaagtt tggcaaacag ttccaggcgg
     3121 ttttcaatgg actcacactt ggactcgtaa aggacttccc gggcattcga gcagagcagc
     3181 acctcctcga tctgagttcc gcgggcttct gtgggggcgg aagcatcgaa cgacgtcgcg
     3241 ccaaagtccg ctgggcgccc cagcccgccg aacccccgcc ccggctgcaa ttcgccctca
     3301 tcccgcagcc gcgctgcttc catcagcagg aattggtatt gcccggaaat ggtgcgacgg
     3361 gccggctcgg caaagggctg gagcttgaat tcgccgccct ttaaagccag caggccgtat
     3421 aacgccattt ccccctccaa ttgcccggtt tgcgcgtgaa taatctctcc ctcgctaata
     3481 tatatctgcc cctgccgctt gccggtggtc accttcaaaa tagacgactt gcgagccagg
     3541 cactccattt gcaacacatc atccaacccc acctgctgca tgactccgcg gaatcccgat
     3601 gccgaccgcg ccgtcgccag ggaatccaac gtgataaaca ggttgtgaaa atcttccggc
     3661 gaagacggct tggccaggaa caagatcgcc cccaggtcca ggcaagtctg gcgcacggtt
     3721 tcctccatgc gttccgaaag catgaccacc tgctgccccg gatgggtgcg tcccagcagg
     3781 cgcaggaact cgacgccgtc cataaccggc atctcgacat ccagcaccac caaattcatc
     3841 gggtgcgctt tcaagtgctc cagggcctgg gagtggttgg tcgccgttcg aatcgcccat
     3901 cgccccccgg acaattcctt gaacgaagcc ccgatggccg ccaggaactg tggatcgtca
     3961 tcgataatca aaaattgatg gtgctgcggg tcgttctctt gcatatcgtc ttagctgccg
     4021 ctctcagttc cgagcgcctg cagtgccatt tgtatcgccg cggccacatc cccgtccaca
     4081 tcggccgcgg tgcgtgccag cgcctgccgg gcccgttcgc cgcccaaacg cccgagcgct
     4141 tcggcagcgg cctggcggac atcccgatcc ggatcctcca gcatgccgat gaagagatga
     4201 gtcgccatgc gcttgcgttt tatcgccggc gaacccaaat ggtcctcggg cgtaaattca
     4261 aacgtgtggt ccgccgccat ctctcccaga ttcaccagta attgggcgac aaagaatcga
     4321 atcgccggct ctgcatcctg gatggccagc tttaattgct ccgccacccc gcgcgtttcc
     4381 ggcagcgagg cccagtccgg atcaatacgg gcgagactgg ccgcggcaat ccgccgcacg
     4441 ctggtaacct catctttcaa agccaggatc aacggcgcca ccccgcgacg atcgccgatc
     4501 cgtcctagcg ccatcgccgc cgcttcgcgc acatcggcat ccgtatcatt gagcgcgctc
     4561 gccagcggtt cgcaggccgc cgggttgttg aaccggccaa gagcgatggc ggcttcctgg
     4621 cgaacctccc attccttgtc acgcagcagt ttgcagatcg ggccgatggc ctccgcggct
     4681 ctcaacaccc ccagcgcttg cgcggccgca actcgcacct gcggatggct gttgcgcagg
     4741 ctggtggtca gcgcatccac agcctcgcct ccgccaacct ttcccaacgc atccaccgcg
     4801 gcaacccgca ccgtcggctc ttcggcttgc aacgctgaac gcaacaactt gattatgcgc
     4861 ggatcggaaa tctggccaag ggcttccacc gccccggccg ccgcctgaac cggccccgtc
     4921 tgaatcgtca attgcaacgc cggaagcgcg tcaaccccgg ctgccgcggc tcgccggtac
     4981 catcccttgg cgttttgaaa ccagatgcgt tcctcccggt ctttaggcgt ccatcgcacg
     5041 gtatcaatcg cttgcgccgc cccgacgcgc acgtgaaaat tcgggtggcg gagcaggcgc
     5101 accaattcga ccaacacccg ctcgtcaacg atctttcgca agccgaacag ggccgcccga
     5161 atcacctccg ggtctttgtc ctccagcgcc ttcagcagcg gttcatagcg cccgggatgc
     5221 tccgaccgtc ccaacgccgt cgccgctatc cgccgcactt ccacatcgga atcggtcaac
     5281 gccgccgttg ccaacgcgct caatgcgcgc gctttaggat cctgcgaaag ctcctgcgct
     5341 gcctttctgc gcgtcgctgc cgtcccggac tttagctgtc gtaacgtcac ccaaagcatg
     5401 caacacccca tcctagccga aagctccagc cctgtcacgg actaagccat gtttttcatt
     5461 cctgagcttc acgccggcgc ctggcattaa atcatcccaa agacaaccct cgcgccgcgg
     5521 cgggatttcc ccggcatcgc cgtatccctg ccactggccg ccaatcccca accagaacat
     5581 ttccttgcgc tgccgcggtc gaaaccccat ttctcaaccg gctatcgctt ttccacccag
     5641 atattgcgat actgcaccgg attgccgtgg tcctgcagca aaaggccccc cggctcgttc
     5701 tcccggtcat tcagcgcgcc tcccgtgacg cccggcagct ccaggttatc ctgcaccacg
     5761 acaccgttat gcaccaccgt aatccgcgcc cgggcggtct tcttgccggc ggcatcgaat
     5821 ttggcagccg taaacgtgat atcatacgac tgccattgca acggcggggc gcacatattt
     5881 acccggggcc ggctcacggt gtaaacgccg ccgcattcgt tgtcctgccc ctccagcccg
     5941 taactgtcca acacctgcac ctcgtagcgt ccctgcaaat aaaccccgct gttagcccgg
     6001 ccctggccaa aggcggtcgg catatacggc aatcggaact ccaggtgaac ccgctgatcg
     6061 aggaacttct cccgggacat gatgtcaccg ccgtttactt ccatcacccc atcgggcagc
     6121 accttccatt tcaccggctt gccctcctcg cggtgccgcc actggtccag gttcttcccg
     6181 tcaaaaagca ctatcgctcc cgccgggggc gccttgccca gttccggcga tatccgttgt
     6241 gtctgcttcc attcaaaagt gccgcgcaac cggcccgcca tcgtcccccg ggcgttcccg
     6301 ccctcaacag cgccggacca caaagccgcg cttagcacca ccccgtttcc cgtggtgcca
     6361 aacacccggc ttatatcaaa ggggatgtca tccatgaacc tgacctgccc gtcgcgaatc
     6421 tcacctttca accggtaaag atacggtccg cgctgaagaa aatctgatac cagtcgcgcc
     6481 tcgtactttc ccccgcccag cgggatcata taaaccccca ccttttggga ttcatcattg
     6541 acgctgatct cgccctgcca atcgcccatg tatgggtccg tcgcctgctg ggcaaacaag
     6601 gtggacgcca ttaacactcc cgccagaatg ccgcccaagt ttgtattcat tcgcatacgc
     6661 caagtctgaa cattccccac caacgcgcaa gcctggaatc cgcccaactc gctcgcggct
     6721 cgtcccggta tagccagccc ccacaagccc ggagccgtgg ccttctacat acttagcggc
     6781 aaaaaccgaa accacgccga caaaaaccgc aacccgcagg ccggcaaggt tctgccccat
     6841 cgcagtgccc agcaagctaa cggttaaatt gcggcttgtg cgaaacgggc cggttctcct
     6901 caagctactc cattcttaac tggcgcgtcc ggcaggactc gaacctgcaa ccttctgatc
     6961 cgaagttcgg acaagcagga ttgtgtccag tcgtcaccag ttgcaccaag ttgcaaatcc
     7021 tcaacattat cagggcattc cgcgtccgat gacaagactc aatgcaactc gatacaactg
     7081 cccaaaaccg aaaaattggc caagaattgg ccaagcattt tccggcttgg gcgacagcgt
     7141 gcgcttctcg tgtagctgat acaccagcgc gctccgattt tctgatggta tcggcgcatg
     7201 agcgccgaaa acatcgctga cgattcaaac gg
//
LOCUS       DAOPQG010000143         7149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_11607_length_7149_cov_12.431209, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000143 DAOPQG010000000
VERSION     DAOPQG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11607_length_7149_cov_12.431209"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..1497
                     /locus_tag="PKI07_12645"
     CDS             571..1497
                     /locus_tag="PKI07_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51933.1"
                     /translation="MLSSKPPSSYSGATSELHRRLNFNTDRASVRRWAIEHQLAPDTR
                     YKPTPKPVKRWQARDYGALWQYDASPHAWLPGGPHKQVLLDILVDATRLNPGARLYPS
                     ETLLAHFDFLSRVFLAHGLPLALYVDYHSFFYAHNPDAFPQLGQALHFYGVHLLYAPT
                     PQAKGKIERRHDYWQKRLVPLLAADQIVERVGANPLFDQLVPHANQPEIHRELGQTPL
                     QAHQQALSENRSALRPAPPCPWWPCVWSQQTRLRVGDDAKVPVGAQRHPIDASPRSTV
                     IRCLRPDGDIYYLRHAPDPNAKPLVLLHCPAF"
     gene            1611..1695
                     /locus_tag="PKI07_12650"
     tRNA            1611..1695
                     /locus_tag="PKI07_12650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1645..1647,aa:Leu,seq:gag)
     gene            1686..2252
                     /locus_tag="PKI07_12655"
     CDS             1686..2252
                     /locus_tag="PKI07_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51934.1"
                     /translation="MRTTPLFLVVPGGRSNPLDPDGATTNDLARNTVLVMDTTLASLR
                     VWPLWQKARHLAVSGVHEDSATGARAEEFCQTLARGLGNKCAISRELWLATELQAASL
                     RAIAAAEAAAADLVIVSVHHAETLPDEVKRWLDLWLKPKRRRPAVLLALFDPLYLGSS
                     AAIQSYLQNAAQRGHREFLARTEEKPFD"
     gene            2269..2604
                     /locus_tag="PKI07_12660"
     CDS             2269..2604
                     /locus_tag="PKI07_12660"
                     /inference="COORDINATES: protein motif:HMM:NF033216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HOC51935.1"
                     /translation="MNPDPHPPAVPPRQAMAKAVCLGLIGLLALTGCAHHYVMRLNNG
                     AEITTASKPQLVEDTYRFKDARGQEHFVSAGRVREITPASMARRESNLPAIKEKKPKQ
                     RKWYRLWLW"
     gene            complement(2617..3225)
                     /locus_tag="PKI07_12665"
     CDS             complement(2617..3225)
                     /locus_tag="PKI07_12665"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOC51936.1"
                     /translation="MFTGIVEETGTVEQIRPAAKSIELTVRTRVCAPGLKVGSSLAIN
                     GCCLTVVKLTRRGPGKLAQFDLLKETWQRTNLQFARPGSQVNLERPLRADGNLGGHFV
                     TGHIDGIGRITRWERVGQDHVLDIAAPPEVMRYVVFKGSMAVDGISLTVAGVQKRAFR
                     IWIIPHTYEVTALRERQVGDAVNLEADLLGKYVEKFIAARGR"
     gene            complement(3276..4451)
                     /gene="ribD"
                     /locus_tag="PKI07_12670"
     CDS             complement(3276..4451)
                     /gene="ribD"
                     /locus_tag="PKI07_12670"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOC51937.1"
                     /translation="MNPRRTRRPEPSRQERDRRFLRLALRLARRGCGATSPNPMVGAV
                     LVRRGQIIGRGWHRRAGGPHAEIEALQQARQKGQTVKGATLYVTLEPCSTQGRTPPCT
                     EALKAAGLRRVVIGATDPNPRHRGRAFAILRRAGIAVTSGILARECAQLNEAFNHWIV
                     RRVPFVTVKAAMTLDGKIATATGESRWITGGPARAWGMKLRQGADAILVGINTVLADN
                     PRLTVRPRPSGARRAASAAADGRLRRIVLDARARTPLRARVVSDAWAPLTTIVVGASA
                     PPRRVAALARRVRVLVAPASRGQINLPWLLRRLGAENVTHLLVEGGGEVNASFLLSGL
                     AQRVVFFYAPKILGGRGARRAVAGEGARARAECLRLTEVEWRRLGADWRMSARVAAA"
     gene            complement(4448..5359)
                     /gene="ftsY"
                     /locus_tag="PKI07_12675"
     CDS             complement(4448..5359)
                     /gene="ftsY"
                     /locus_tag="PKI07_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOC51938.1"
                     /translation="MGLFAKFKAGLQKTHSKLAHEIKRIVTRSPKLDAAGLEELEGAL
                     LAADLGPAVSGQILRAVRQAYETQGSAGREVFAIARREVERCLGAEPAELRRAPQGLT
                     VVSIVGVNGTGKTTTAAKLAHRVQTRGHKALLAACDTFRAAAIEQLRLWGQRLQVEVI
                     AGAYGADAAAVAHDAVAAGQARGVDYLLVDTAGRLHTKHNLMQELQKLHRVMNRQLPG
                     APHEVLLVLDATTGMNALNQAREFNKAVPLSGLIVTKLDGTSKGGMVVAIQQELNLPV
                     KYLGLGEQADDLQPFDAKQFAGALFGE"
     gene            complement(5421..6302)
                     /locus_tag="PKI07_12680"
     CDS             complement(5421..6302)
                     /locus_tag="PKI07_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOC51939.1"
                     /translation="MKPRRAGETPMSFADLHLHSRYSDGTYGPEELVTEARRCGLAVI
                     ALTDHDSVEGCAAAARACAAAGLEFIPGVELTVEVGGDELHLLGYFIDTQNGFLRSRI
                     KQFQVARQNRIREMVARLHRLQVPLSEQSVFALANCQSPGRPHVARALVQAGLCRSLD
                     EAFERFLRYNRPAWVPKFKMDGREAIELLHQAGGVAVLAHPGLNRSDAVIPGLVEAGL
                     DGLECFHTKHSAATANLYLELAARLGLVVTGGSDCHGLSKGRPLIGTVKVPCQRLEPL
                     RARAAELRRAARPSAQP"
     gene            complement(6299..6799)
                     /gene="nusB"
                     /locus_tag="PKI07_12685"
     CDS             complement(6299..6799)
                     /gene="nusB"
                     /locus_tag="PKI07_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOC51940.1"
                     /translation="MGKRREARERAVQFLFQYDLNPGEDLPAALAHFWESQRAAAIAE
                     DKGGATWGQPQELPPPTVEELEVRAFAEPLIQGALQHRNEVDAVIRKHARNWELHRIA
                     VVDRNILRLAIYEMLYREDIPPVVSINEAVDIAKKFSTQDSGKFVNGILDQVKGELMR
                     PARIAQ"
BASE COUNT         1190 a         2626 c         2125 g         1208 t
ORIGIN      
        1 ctaaaagccc tcctttcagc ctcatttttc tgccgattga aatgtcgtgg tgcgatcaaa
       61 gttggagaag agtgtgcgat caaagttgga ataatggggg ggtagcctct gtttcccttg
      121 cgggatcacc gtcagggcat ccaaagtatc tttgcttccg cggaggataa ggctctgcgg
      181 ggacgcaacc tgagcgccgt cggaagcctg ataaacggcc agtcgggctg ccgcacggct
      241 gcccggctag ccctggccgg gatcagttcc caagcaataa atccatcttc ctgctatctg
      301 ggcgcgatga aaaacatcag ctcgctcacc aactcgctca tcgcgtcgcc gcgccactga
      361 tccgccatct cttcgcgcaa gcccgttccg gccggctctc gcccgaggcg gtcgcgctcg
      421 aattgggcat cagtgtgagc gcttctacca actgcgtacc gcctacctgc acgcctgcgc
      481 tcaaggccac gctgaaacct ggtcgcccgg tctctcgggg gaggatcatc cccctcaagg
      541 gacggccgaa gtcacggccc tgcccccaaa ttgctctcct ccaaaccccc gtcctcctac
      601 agcggcgcca ccagcgaact ccaccgccgc ctcaacttca acaccgatcg cgccagcgtc
      661 cgtcgttggg ccatcgaaca tcaactcgcc cccgacaccc gttacaaacc cacccccaaa
      721 cccgtcaaac gctggcaagc ccgcgactac ggcgccctct ggcaatacga cgccagcccc
      781 cacgcctggt tgcccggcgg ccctcataaa caagtcctgt tggacattct cgtcgacgcc
      841 acccgcctca accccggcgc ccgcctctac cccagcgaaa cgctcctggc ccactttgat
      901 tttctctccc gcgtcttcct cgcccacggc ctgcccttgg ccctctacgt ggactaccac
      961 tccttcttct acgcccacaa ccccgacgcc ttcccccaac tcggtcaagc tctccacttc
     1021 tacggcgtcc acctcctcta cgccccaacg cctcaagcca aaggtaaaat cgaacgccgc
     1081 cacgactact ggcaaaaacg cctcgtcccg ctcttggcgg cggaccagat cgtcgaacgg
     1141 gtcggggcca accccctctt cgaccaactc gtcccccatg ccaatcaacc tgaaatccac
     1201 cgcgaactcg gccagacccc gctccaagcc caccaacaag ccctctccga aaatcgctcc
     1261 gccctccgcc ccgcgccccc ttgtccctgg tggccctgcg tctggagcca gcaaacccgc
     1321 ctgcgggtcg gcgacgacgc caaagtcccc gtcggcgccc aacgccatcc cattgacgct
     1381 tcaccccgct ccaccgtcat ccgctgcctc cggcccgatg gcgacattta ctacctccga
     1441 cacgccccag acccaaacgc caaacccctc gtcctactcc attgcccagc cttctgatcc
     1501 cattccaact ttgttcgcac acccttttcc aactttgatt gttaacgaca cggcggcgaa
     1561 tttggccttg caccccaccc cgggaactgc ttctttattg gcagttctgt gcgcatatgg
     1621 cggaattggc agacgcgcta ctttgaggtg gtagtggagt aatccgtgca ggttcaagtc
     1681 ctgctatgcg caccactcct cttttcctgg tcgtcccggg cgggcgatcg aatccacttg
     1741 acccggacgg ggcgacgacg aacgatttgg cgcgaaacac agttttggtc atggacacga
     1801 cactagccag cctgcgggta tggccgcttt ggcagaaggc ccggcacctc gccgtttccg
     1861 gggtgcatga ggactcggcc acgggcgcgc gcgcggagga gttttgccaa accctggccc
     1921 ggggcctggg aaacaaatgc gccatttccc gggagctatg gctggcgacc gaattgcagg
     1981 ctgcttcgct gcgggcgatt gccgccgcgg aagcagccgc ggccgacctg gtgattgtct
     2041 ccgtgcatca cgccgaaacg ttgccggatg aagtcaagcg ctggctggat ttatggctca
     2101 aacccaagcg ccgccgcccc gccgtgcttc tcgcgctttt tgatccgctt tacctgggca
     2161 gttccgccgc gatccagagc tatctgcaaa atgccgccca gcgcgggcac cgggagtttt
     2221 tggcgcgcac ggaagaaaag ccgttcgact gagggcggct ccgaaattat gaatcctgac
     2281 ccccatcccc ccgcggttcc gccgcgccag gctatggcca aagccgtttg cctgggattg
     2341 atcggcctgc tggcgctgac cggatgcgcg catcattacg tgatgcggct caacaacggc
     2401 gcggaaatca ccaccgcctc caaaccgcag ctcgtggagg atacctaccg gttcaaggat
     2461 gcccgggggc aggagcattt cgtgtccgcg ggccgcgtgc gcgagattac gcccgcctcg
     2521 atggcccggc gcgaaagcaa tctgccggcc atcaaagaga agaaacccaa acagcgcaag
     2581 tggtatcgct tgtggctgtg gtgatccgcg ccgggctcag cggccccggg cggcgatgaa
     2641 tttttccaca tatttgccga gcaggtcggc ttcgaggttg accgcgtcgc ccacctggcg
     2701 ctcgcgcagg gcggtaacct cgtaggtgtg ggggatgatc cagatgcgga aggcgcgttt
     2761 ttgcacgccg gcgacggtaa ggctgatgcc gtccaccgcc atggagcctt tgaacacgac
     2821 gtagcgcatc acttcgggcg gggcggcgat gtccagcacg tgatcctggc ccacccgttc
     2881 ccagcgggtg atgcggccga tgccgtcaat gtggcccgtc acgaagtgtc cgccgagatt
     2941 gccgtccgcg cgcagcggcc gctccaggtt cacctgcgag ccgggccggg cgaactggag
     3001 gttcgtgcgc tgccaggttt ccttcagcag gtcaaattgc gccagcttgc cggggccgcg
     3061 ccgggtcagc ttgaccaccg tcaggcagca gccattgatc gccaggctgg agcccacttt
     3121 cagccccggc gcgcaaaccc gggtccgcac cgtgagctcg atggatttcg ccgcgggccg
     3181 gatttgctcc acggtcccgg tttcttcaac gatgccagta aacatggcgc catagtaagc
     3241 ggccgggcgc cccggtgaaa tggcaaaaag cggcctcagg ccgccgccac gcgggcgctc
     3301 atccgccagt ccgcgccgag ccgccgccac tccacttcgg tcaggcgcag gcattccgcc
     3361 cgcgcccggg ccccctcgcc ggcgaccgcc cggcgcgcgc cgcgcccgcc caggattttc
     3421 ggcgcataga agaagacgac gcgctgggcc aggccggaca gcagaaatga ggcgttcact
     3481 tcgccgccgc cttccaccag caggtgggtg acgttctccg cgccaagccg ccgcagcagc
     3541 cacggcaaat tgatctgtcc gcgggaggcg ggcgcgacca gcacccgcac ccgccgggcc
     3601 agggccgcga cgcggcgcgg cggggccgac gcgccgacca cgatggtggt cagcggcgcc
     3661 caggcgtcgc tcacgacccg cgcgcgcaac ggcgtccgcg cccgcgcatc caatacgatg
     3721 cggcgcagcc ggccatccgc cgcggccgag gccgcccggc gcgccccgga tggccgcggg
     3781 cggacggtca ggcggggatt atcggccagc acggtattga tccccaccag gatcgcatcc
     3841 gccccctgcc gcagtttcat tccccaggcc cgggccggcc cgccggtaat ccagcgggac
     3901 tcgcccgtgg cggtggcaat cttgccgtcg agggtcatgg cggccttgac ggtcacaaag
     3961 ggaacgcgcc ggacaatcca gtggttgaac gcctcgttga gctgcgcaca ctcccgggcc
     4021 aggatgccgg aggtgaccgc gatgcccgcg cggcgcaaaa tcgcaaacgc gcggccgcgg
     4081 tgccgggggt tggggtcggt cgcgccgatc acgacgcgcc ggaggcccgc cgctttgagg
     4141 gcctcggtgc agggcggggt gcgcccctgc gtgctgcacg gctccagcgt cacatagagc
     4201 gtcgcccctt tgacggtttg ccctttttgc cgcgcctgtt gcagcgcctc gatttccgcg
     4261 tgcggcccgc ccgcgcggcg atgccagccc cgcccgatga tctggccgcg ccggaccaac
     4321 acggctccga ccatgggatt gggcgaggtg gcgccgcaac cccggcgggc caaccgcagg
     4381 gccaggcgca ggaagcggcg atcccgctcc tggcgggatg gctccggccg ccgcgtgcgc
     4441 cgtgggttca ttcgccaaac aacgccccgg cgaactgctt cgcgtcgaag ggctgaaggt
     4501 cgtcggcctg ctcgcccagc ccgaggtatt tgaccggcag gttcagttcc tgctggatcg
     4561 ccaccaccat gccgcctttg ctggtgccgt cgagcttggt gacgatcagc cccgagaggg
     4621 gcacggcctt gttgaattcc cgcgcctggt tgagcgcgtt catgccggtc gttgcgtcca
     4681 gcacgagcaa tacctcatgc ggcgcgcccg gcaattgccg gttcatgacc cggtgcagct
     4741 tctgcaattc ctgcattaaa ttatgcttgg tgtgcagccg gcccgccgtg tccaccagca
     4801 ggtaatccac cccgcgcgcc tgcccggccg ccaccgcgtc gtgggccacg gccgccgcat
     4861 ccgcgccgta ggccccggca atgacctcga cctgcagccg ctgcccccag agcctgagct
     4921 gctcgatggc cgcggcgcga aacgtatcgc acgccgccag caatgcctta tgcccgcgcg
     4981 tttgcacccg gtgcgccagc ttggccgccg tggtggtctt gccggttccg ttgacgccca
     5041 caatcgagac caccgtcagg ccctgcggcg cccggcgcag ctccgccggc tccgcgccca
     5101 ggcagcgctc gacttcgcgc cgcgcgattg cgaagacttc ccgccccgcg ctcccctgcg
     5161 tctcgtacgc ctgccggacc gcgcggagga tttggccgct caccgcgggg cccaggtcgg
     5221 cggccagcag cgcgccttcc agttcttcaa gcccggcggc gtccagtttg ggcgagcggg
     5281 tgacaatgcg tttgatttcg tgcgccagct tgctgtgcgt cttttgcaaa ccggctttga
     5341 atttggcaaa gagtcccata ggctatctca aaagtcgtca ggctgaaaac ggcgccgggc
     5401 gggagggaag ggcggaaggg tcatggctgg gcggaaggcc gcgcggcgcg ccgaagctcc
     5461 gccgcccgcg cccggagcgg ttcgaggcgc tggcacggca ctttgaccgt gccgatcagc
     5521 ggccggcctt tgctcaggcc gtggcaatcc gagccgcccg tcaccaccaa cccgagccgg
     5581 gccgccagct ccaggtacag gtttgcggtc gccgcggaat gcttggtgtg aaaacactcc
     5641 aggccatcca gcccggcttc gaccaggccg ggaatgacgg cgtcggagcg attgagcccc
     5701 ggatgcgcca gcaccgccac gccgcccgcc tggtgcaaca gctcaatggc ctcgcgcccg
     5761 tccattttga acttgggcac ccaggccggg cggttatagc ggagaaaccg ctcaaacgcc
     5821 tcgtccaggc tccggcacaa gcccgcctgc accagcgccc gcgccacatg cggccgcccg
     5881 ggcgactggc agttggccag cgcaaacacg ctttgctccg acagcggcac ctgcagccga
     5941 tgcagccgcg ccaccatttc gcgaatccgg ttctggcgcg ccacctggaa ctgtttaatc
     6001 cgcgaccgca ggaagccatt ctgcgtgtct ataaagtagc ccaggagatg cagttcgtcc
     6061 ccccccactt ccaccgtcag ttccaccccc ggaataaact ccaggcccgc cgccgcgcag
     6121 gcccgcgccg cggcggcgca gccctccacc gaatcatggt cggtcaacgc gatgaccgcc
     6181 aatccgcagc gccgcgcctc cgtgaccagt tcctccggcc cataggtgcc atccgagtat
     6241 cggctgtgca gatgcagatc ggcaaatgac atcggcgtct ccccggcgcg gcgcggcttc
     6301 attgggcaat ccgggccggc cgcatcagct cgcctttcac ctggtcaagg atgccgttga
     6361 caaacttgcc gctgtcctgc gtggagaact tcttggcgat gtccaccgcc tcgttgatgc
     6421 tgaccaccgg cgggatgtcc tcgcgataca gcatctcata aatggccagc cgcaggatat
     6481 tgcgatccac caccgcgatg cggtgcagct cccagttccg cgcatgtttc cggatgaccg
     6541 cgtccacctc attgcgatgc tgcaacgcgc cctggatgag cggttcggca aacgcgcgca
     6601 cctccagctc ctccacggtg ggcggcggca gttcctgcgg ctggccccac gtcgcgccgc
     6661 ctttgtcctc ggcaatggcc gccgcccgct gtgattccca gaaatgcgcc agcgccgccg
     6721 gaagatcctc tcccgggttc agatcatact gaaacaggaa ctgcaccgcg cgctcgcgcg
     6781 cctcacgtcg tttgcccatg gcattaccat gccgcagatt gggatgtcag gcaatgccgc
     6841 aacgcaatct ggctccgtcc cggcaacatc cccggaactt actctcccag cctccttccg
     6901 tgcaaagcga caccaaggct ggcgcatcgg atgccccgtg gccgtgggaa cgagccgttc
     6961 caataaagcc ataccggttt aaccccccgc ccggggatgg gtctctattc catcccgccc
     7021 cctgcaggcc gcctaccccc acgcggcgct ttgcccccgc ctgccaccgc caacccccca
     7081 aaattgccct tgcacgaaga cctcccgccc gccagccttc ctccagaccc ttggggtggg
     7141 ggcggctgg
//
LOCUS       DAOPQG010000144         7146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_11612_length_7146_cov_14.071217, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000144 DAOPQG010000000
VERSION     DAOPQG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11612_length_7146_cov_14.071217"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PKI07_12690"
     CDS             <1..611
                     /locus_tag="PKI07_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOC51941.1"
                     /translation="IPIEEMYPRIGGFVGFHPMCELPLTFFTRIVGEGGVHQFVFLER
                     VEGYGVQIMKGYNFDPRDTMWIFSHSGINNVNIDVALESKKIGMKVIAYGSAAAAKGK
                     QTRHSSGKTIFDIADLVVDTCAPIGDSCVPLKNHQDKVGPVSTMAFITCVWMTVCTVA
                     EILADRGVKLYINPSHNVPGDTTARERLDAALAEYKRRIAGV"
     gene            631..1929
                     /locus_tag="PKI07_12695"
     CDS             631..1929
                     /locus_tag="PKI07_12695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOC51942.1"
                     /translation="MNLISRTSMTGSHPLTRRRFLYAMAATASGALLPGIGRFRANAA
                     DNPVRFPSTEHFWYRLAPPGRYIDSQRGRKAFAYADDRLFLSGDNGRTWPRSLAFPDA
                     RQITFSHILKNGNILFATRTKLYLSTDNLKTYRPVVVKAQDGSDYTPHTPQNPANPGW
                     YFHTLPGIISWKVRGREMLVWGNYCNVRGGAAPVNIYYSIDGGRTVKIAYAFGQNPHF
                     RDNGSSGGGPTGALLGNPDNPVICRHVHAVAYNPAEDAFYACTGDLDRGAGHECHWLK
                     GIYQAQTDTWRWTVLVSTQSNTRYKSGGITFVKGRAYWISDANGPEPHDRGIFCCAAA
                     DIPYPERHTRLYNPQVESGCMIIQDGFILASHCAPASPLKTGFIVSPDMGKTWAQHDL
                     AELGHRSPVRFHEKNREGWIRVDLRSGWIDRAEVLFIKPK"
     gene            complement(1996..3792)
                     /locus_tag="PKI07_12700"
     CDS             complement(1996..3792)
                     /locus_tag="PKI07_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51943.1"
                     /translation="MRTRRRFILGSATGVALLALTAWTQVLDLPPRAPNAVSGSEFAR
                     RVATLDLGERDREVYAQVMAGNVPDFLRRLCPVPARSVVEGRINSGTYYVTPDYLAVG
                     SDDDYFLAPISPYTAQRIAEALHCTLPTPKMVNQIFFAAEVKLAPAPIPPSAAMTTVP
                     VFRQHNDLVRAQRAEQLSAHPLGALVAGHKKDVVLTARLAANPGKVAIYGWHRTNGLP
                     IQPLYLKHSAAWVDYSQCVRLVQQKMVANETARTVAEVLADPALAGLLSDEGPLAAVR
                     YPTNAPLALQTLTNQASVNRLMTNAAFDERLTSFTFNPEVKVRVNLPGEAAFAAAGKV
                     LLIFYALPNGNSTDQTAGRIPRPGDDWRFDIQHIDAQTRFLRDLIKDRAVVVVYLEVA
                     GKSWPAWRKAHGDRQIPEIVATVKKLFPGRAVEVVLNGHSGGGSFIFGYLNAVDRIPD
                     DVVRIAFLDSNYAYSRAQGHLDKLVKWLSSPRQPSLCVLAYNDAAALLNGTNFVSAAG
                     GTWGKSHAMLRDLAGTFQFTSQTNAGLRKHWALDGRVQFLLKENPERKILHTVQVERN
                     GFIHSLVSGTTNEGRGYEYFGPRAYTRWIRPE"
     gene            complement(3795..5351)
                     /locus_tag="PKI07_12705"
     CDS             complement(3795..5351)
                     /locus_tag="PKI07_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOC51944.1"
                     /translation="MSKRFYITTAIDYVNGHPHLGHAYEKVVADVIARARRSLGREVF
                     FLTGLDEHGQKVQQAAVAEGKKPQAYCDELAAVWQAFAARLNLSHDDFVRTTQPRHRQ
                     IVQAILAKLHAEGHFYRETYRGFYSTRQETFLTHKDRLPDGSFDPSWGEVVELEEENY
                     YFKLKDHQSWLIDHIERNPAFICPDARRNEVLGFLRNNVLEDLCISRPATRLNWGIPL
                     PFDPDYVTYVWFDALANYITIPAAQGDPGVPGPLRPPVSPGGHPLALWPADVHLIGKD
                     IVKFHAVYWPIMLKAMGAPLPRQVLVHGWWQKDGQRMSKSTGNVVDPVAVIDEWGVDA
                     FRFYVLRELDIGPDGNWTEAGFEARYAAELANGLGNLVNRSLSMLKRYRSGVVPGVAK
                     ELAPDAERAIAETLSRLEQNQLQGALQAIWSLITRANQYVDQTAPFKLAKDPARAESL
                     DQVLYSLAETCRILAVLLWPFLPDTAGKIYAQLGLPGAPDRAAAARWGQLPAGHAIGT
                     PAPLFPRKEI"
     gene            complement(5394..6524)
                     /locus_tag="PKI07_12710"
     CDS             complement(5394..6524)
                     /locus_tag="PKI07_12710"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC51945.1"
                     /translation="MMRRAIAGLLLFGLGSLFAGAGNQDWQQPPARVPANAPQLSRLL
                     VDEHGRRIASKRGWRQQRALLQAQWQAVLGEFPAAKPPLRSQVLGTEELPEFTRQYAT
                     YQVEEGVFTDGYLLTPRAAKGRLPAVVVFHPTTPLQAKGVAGLAAEYDREKWQGVQLV
                     QRGYIVWCPRNYIETAGTNWAGNAARVRARHPRWTGMTRMVWDAIRAADFVTSLPRVD
                     RKRLGCLGHSLGGKEVLFAMAFEPRYRAGVSSEGGIGLKFSNWEAPWYFGRQIQQPGF
                     QRENHEVLALAAPRAFLLLAGDSADDDRSWEFIQAARPVYELLGAARNLGWLNHHQGH
                     RYAPEARAAAEAFLDQHLKPRPREHFDRAPPGGKGGGRSSGR"
BASE COUNT         1328 a         2424 c         2135 g         1259 t
ORIGIN      
        1 ccattcccat cgaggaaatg tatccgcgca tcggcggctt tgtggggttc caccccatgt
       61 gcgagctgcc gctgaccttc ttcacgcgca tcgtgggcga aggcggcgtg caccagtttg
      121 tattcctgga gcgcgtggag ggctacggcg tgcagattat gaagggctac aacttcgacc
      181 cgcgggatac gatgtggatt ttctcccact ccggcatcaa caatgtcaac attgacgtgg
      241 cgctcgaatc caagaagatc ggcatgaagg tcatcgccta cggctccgcc gccgcggcga
      301 aaggcaagca gacccgtcat tcctcgggca agaccatctt cgacattgcc gacctggtcg
      361 tggacacctg cgcgccgatt ggcgactcct gcgtcccgct gaagaatcat caggacaaag
      421 tggggccggt atccaccatg gcctttatca catgcgtctg gatgacggta tgcacggtcg
      481 ccgaaatcct ggcggaccgc ggcgtgaagc tctatatcaa cccgtcccac aacgtccccg
      541 gcgataccac cgccagggag cgcctcgacg ccgcgctggc ggaatacaaa cggcggattg
      601 ccggcgtctg acggcttccg gccctggccc atgaacctta tttcccgcac ttccatgacc
      661 ggctctcacc ccctcacgcg ccggcgtttc ctgtatgcca tggcggcgac cgcgagcggc
      721 gccctgctgc cgggaatcgg ccggttccgg gcgaacgccg ccgacaaccc cgtccggttc
      781 cccagcaccg aacacttctg gtatcgcctg gcgcccccgg gccggtacat tgactcgcag
      841 cgggggagga aagccttcgc ctatgcggac gaccgcctct ttctctccgg ggacaacggg
      901 cgcacctggc cgcgcagcct ggcgtttccc gacgcccggc agatcacctt cagccacatc
      961 ctgaagaatg ggaacatcct ttttgcgacc cgcacaaagc tgtacctcag cacggataac
     1021 ctcaaaactt accgtccggt tgtggtcaag gcgcaagacg gctcagatta caccccgcat
     1081 acgccgcaaa acccggccaa ccccgggtgg tacttccaca cgctgccggg catcatctcc
     1141 tggaaagtgc gcggccggga aatgctggta tggggaaatt actgcaacgt ccggggcggc
     1201 gccgcgcccg tcaacatcta ctactcgatt gacggcgggc gcacggtgaa aatcgcctat
     1261 gctttcgggc aaaatccgca ctttcgggac aatggctcat caggtggcgg tccgacgggc
     1321 gcattgctgg gcaatcccga taacccggtg atctgccggc atgtccatgc ggttgcttac
     1381 aaccctgccg aggacgcctt ctatgcgtgc acgggcgatt tggatcgcgg ggcggggcat
     1441 gaatgtcatt ggttgaaagg catttaccag gcgcaaacgg atacctggcg ctggacagtc
     1501 ctcgtttcca cacaatcaaa tacccgctac aagtccggcg gcatcacctt tgtgaagggt
     1561 cgagcctatt ggattagcga cgccaacggg ccggaaccgc acgatcgggg aatcttctgc
     1621 tgcgccgcgg cggacatccc ctaccccgag cgacacaccc ggctctataa tccccaggtc
     1681 gaatccggct gcatgatcat ccaggacggc tttatcctgg catcgcactg cgccccggct
     1741 tcgccgctga aaaccggctt catcgtctct ccggacatgg gcaagacctg ggcgcaacac
     1801 gacctggcag agctcggcca tcgctcgccg gttcgcttcc atgagaaaaa ccgcgaggga
     1861 tggatccggg tggatttgcg ctccgggtgg atagaccgcg ccgaagtgtt gttcatcaag
     1921 cccaaataag gggtggccgc ccgcgccgcc cccggcaggg agagcgcgcc cgcgccgttt
     1981 cccggggcct tccctttact cggggcggat ccagcgggtg taggcgcgcg ggccgaaata
     2041 ttcatagccc cgcccctcgt tggtcgtccc ggacacgagg ctgtgaatga agccgtttcg
     2101 ctccacctgc acggtatgaa gaatcttccg ctccgggttt tccttgagga ggaactgcac
     2161 gcggccatcc agcgcccagt gcttccgcag gccggcgttg gtctggctgg tgaactggaa
     2221 agtcccggcc aggtcgcgca gcatggcgtg gctcttgccc caggtgcccc ccgccgcact
     2281 gacaaagttc gtgccgttaa gcaacgctgc cgcgtcattg taggcaagca cgcagaggct
     2341 gggctgccgc ggcgaggaca gccatttcac gagcttgtcc aggtgcccct gggcgcggct
     2401 gtacgcgtaa ttgctgtcca gaaaggcaat gcgcaccacg tcatcgggaa tccggtccac
     2461 cgcattcagg tagccaaaga tgaaactgcc gccgccgctg tggccgttca gcacgacctc
     2521 caccgcccgg ccgggaaaca gcttctttac cgtcgccacg atctccggga tttgccggtc
     2581 gccatgcgct ttgcgccagg cgggccagct tttcccggcc acttcgaggt aaacgaccac
     2641 caccgcgcga tctttgatca aatcgcggag gaagcgggtt tgggcgtcaa tgtgctggat
     2701 gtcaaaacgc cagtcgtcgc cgggccgcgg aatccggccc gccgtctggt cggtgctgtt
     2761 gccgttgggg agggcgtaaa aaatcagcaa caccttgccg gccgccgcaa aagcggcttc
     2821 ccccggcaaa ttaacgcgaa ccttaacctc cgggttgaag gtgaacgagg tgaggcgctc
     2881 atcaaacgcc gcgttcgtca tcaaccggtt cacactggcc tggttggtta acgtctgcag
     2941 cgcaagcggc gcattggtgg gatagcggac ggcggccagc gggccttcat cactcagcag
     3001 gcccgccaaa gcgggatccg ccaggacttc ggccaccgtg cgcgccgttt cattggcgac
     3061 catcttctgc tgcaccagcc ggacgcactg gctgtaatcc acccaggccg ccgaatgctt
     3121 cagatacagc ggctggatgg gaaggccgtt ggtccggtgc cagccataga tggccacctt
     3181 gcccggattg gccgccagcc tggccgtgag caccacgtcc ttcttatgcc cggccaccag
     3241 cgcgcccagg ggatgcgcgg acaactgctc cgctcgctgg gcgcgcacca ggtcgttatg
     3301 ctggcgaaaa accgggaccg tggtcatggc ggcgctggga gggatgggcg cgggcgccag
     3361 cttgacctcg gcggcaaaga atatctggtt gaccattttc ggcgttggca gcgtgcagtg
     3421 cagcgcctcg gcaatccgct gggcggtgta gggcgaaatc ggcgccagga aatagtcatc
     3481 atcggaaccg acggccaggt aatccggcgt cacataatag gtgccgctgt taatccggcc
     3541 ctcgacgacg cttcgagccg gcaccgggca caaccgccgt aggaaatccg ggacgttgcc
     3601 cgccatgacc tgggcgtaaa cctcccgatc gcgctcgccg aggtcgagcg tggccacgcg
     3661 ccgggcgaac tccgaaccgc tcacggcgtt cggagcgcgg ggaggcagat ccagcacctg
     3721 cgtccaggcg gtgagggcga gcagcgcaac gccggttgcg ctccccaata tgaatcgtcg
     3781 ccgggtgcgc atggttaaat ctcctttcgc gggaacagcg gggcgggagt tccgatggca
     3841 tgccccgccg gcaactgccc ccaacgcgcc gccgccgcgc gatccggcgc gcccggcagg
     3901 ccgagttgcg catagatttt cccggccgtg tctggcagga aaggccagag cagcaccgcc
     3961 agtatccggc aggtctccgc caggctatac aaaacctggt ccaggctttc cgcccgggcc
     4021 ggatctttcg ccagcttgaa cggcgcggtt tggtccacat attggttggc gcgggtgatg
     4081 agcgaccaga ttgcctggag cgcgccctga agctggtttt gctccagccg gctgagcgtc
     4141 tccgcgatcg ccctttcggc atccggcgcc agttccttcg ccaccccggg caccacgccg
     4201 ctccggtagc gcttaagcat ggatagcgaa cgattgacca ggttccccag gccattggcc
     4261 aattcggcgg cgtagcgcgc ctcgaaaccc gcctccgtcc agttgccatc ggggccgata
     4321 tccagttccc gcagcacgta aaagcggaag gcgtccacgc cccactcgtc aatgaccgcg
     4381 accgggtcca ccacgttgcc ggtgctcttg ctcatccgct ggccatcctt ttgccaccag
     4441 ccatgcacca acacctgccg gggcagcggg gcgcccatcg ccttgagcat gatgggccaa
     4501 tagaccgcat ggaacttcac gatatccttg ccgatcaaat gcacgtcggc gggccagagc
     4561 gccaggggat gcccgcccgg cgagacgggc gggcgcaacg ggccgggcac gccgggatcg
     4621 ccttgcgcgg cgggaatggt aatatagttg gcgagcgcgt cgaaccagac gtaggtgaca
     4681 tagtccggat caaacggcag cgggatgccc cagttcaggc gcgtggcggg gcggctgatg
     4741 cacaggtctt ccaacacgtt gttcctgaga aacccgagca cctcgttccg gcgcgcgtcc
     4801 gggcagataa aagccgggtt ccgctcgata tggtcaatca gccacgactg gtgatctttg
     4861 agtttgaaat agtagttctc ctcctccagt tcgacgacct cgccccagga cggatcgaag
     4921 gagccgtccg gcaggcggtc tttgtgcgtg agaaaggtct cctggcgggt cgaatagaag
     4981 cctcgatagg tttcccggta gaaatgcccc tcggcatgca gtttggcgag gatggcctgc
     5041 acgatctgcc gatgccgcgg ctgggtggtg cgcacaaaat cgtcgtggga caggttcaac
     5101 cgggcggcaa acgcctgcca gaccgccgcc aattcatcac agtaggcctg tggcttcttg
     5161 ccctcggcca ccgccgcctg ctggactttc tggccatgct cgtccaggcc ggtgagaaag
     5221 aacacctccc gccccaggct gcgccgcgcg cgggcgataa cgtcggccac caccttctcg
     5281 tacgcatggc cgagatgcgg atgcccgttg acgtagtcaa tggcggtcgt gatataaaag
     5341 cgcttgctca tcggccccta ctttgaccga ccgcagcctc cttggcaact gcttcaacgg
     5401 ccggaactcc gcccgccccc cttgcctccc ggcggcgccc ggtcgaaatg ttcccggggc
     5461 cggggcttca ggtgctgatc cagaaaagcc tcggcggcgg cccgggcctc gggcgcatag
     5521 cgatgcccct ggtggtggtt gagccagcca aggttccgcg ccgcgcccag cagttcatag
     5581 acgggccggg cggcctgtat gaactcccag ctccggtcat cgtcggccga atctccggcc
     5641 agcaacagaa aagcgcgcgg cgccgccaac gccagcacct cgtggttctc gcgctgaaaa
     5701 cccggctgct ggatttgccg cccgaaatac cagggagcct cccaattgct gaacttcagc
     5761 ccaatacccc cctcgctcga tacgcccgcc cggtagcgcg gctcaaaggc catcgcaaac
     5821 agcacttcct tgccgccgag cgaatgccca aggcagccca ggcgcttccg gtccacgcgc
     5881 ggaagggacg tgacgaagtc cgccgcccgg atggcatccc aaaccatgcg ggtcatgccc
     5941 gtccaccgcg gatgccgggc gcgcacacgg gcggcgttgc cggcccagtt tgttcccgcg
     6001 gtctcgatgt aattgcgcgg gcaccaaacg atgtatccgc gctgaaccaa ctgcaccccc
     6061 tgccattttt cccggtcata ttccgccgcc aaacccgcga cgcctttagc ctgcaacggg
     6121 gtggtgggat gaaagacaac caccgcggga agccgcccct tcgccgcccg gggcgtcagt
     6181 aaatagccgt cggtgaatac tccctcctcc acttggtagg tggcgtattg gcgggtaaac
     6241 tccggcaact cctcggtgcc aagcacctgg ctccggagcg gaggcttggc cgccgggaat
     6301 tcgcccagga ccgcctgcca ttgcgcctgc aataacgcgc gctgttggcg ccagccccgt
     6361 ttggaagcaa tccgccgccc gtgctcatca accagcaggc gggagagctg cggcgcgttt
     6421 gccgggacac gcgcgggcgg ctgttgccaa tcctgattac ccgcgcccgc aaacaggctc
     6481 cccaaaccaa acaggagcag gccggcaatc gcgcgtcgca tcattggccc attgtgcttt
     6541 cccggcggga cgccgcaagc ctccttttca ttaggacaac cccggcgtca ggggcttttg
     6601 ttatcggtgc ctgagtttct gatgtgagag agaaacgata tttccagacc ccaagtcttt
     6661 gccacgcggc aaaacgtcct ctccaagcaa agcgctgaac ctccgcccac cccgcagccg
     6721 caaccactcc agctcctgat cctcacaacc aaagcccctg aacagattcc caaaccaatc
     6781 ctgccaatca ctcaccctgc gcgaatagcc cggggctaaa tctccaacca aactcatccc
     6841 aatcggacga ttacaccgcc tccgatgaag acaacgcaag caatcacctt gcggcaacct
     6901 tcgcccggga ggggaaatgt cctcgccagt cttggcccca tcccgccgaa aaaccagggc
     6961 acgcgacaac acccatccgc ccgttccggc tttgccggac gttcgcgtcc acttcctccc
     7021 gccttggaac ctcgttttac atcatccgca agtcgtccat tcctcctttc cggctcaggc
     7081 tcgcgcccaa gcccgcccca cccgccccag ccagcctaac gctcccatta ggccgtgtac
     7141 aacggg
//
LOCUS       DAOPQG010000145         6894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_12136_length_6894_cov_13.783155, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000145 DAOPQG010000000
VERSION     DAOPQG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12136_length_6894_cov_13.783155"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..1115)
                     /locus_tag="PKI07_12715"
     CDS             complement(372..1115)
                     /locus_tag="PKI07_12715"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOC51946.1"
                     /translation="MAADNCKLMKLCDLTLPTPEENLAADEALLDLCEAGGCGEVLRF
                     WSPRHYFVVLGYGNKAATEANLPFCREQGIPVLRRCTGGGTVLQGPGVLNYALILRIA
                     GNQPCQSIAATNQFILERHRDALAPVLAAPIAWRGQTDLAIDELKFAGNAQRRRRRFL
                     LFHGSFLLRLDLALVEKTLPPPSLQPDYRLNRVHGDFLLNLNVSEDAVKTALAKAWKA
                     ATPLTAIPRDHIHRLVAEKYSLREWNFRV"
     gene            1276..3939
                     /gene="aceE"
                     /locus_tag="PKI07_12720"
     CDS             1276..3939
                     /gene="aceE"
                     /locus_tag="PKI07_12720"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOC51947.1"
                     /translation="MDSRSEEENWLDSLETVLEFALKDLKPQQTSRFLDGLTERLRAR
                     GKEVPYVVGTPYINTIPEEKEAPFPGDWEMERRIKSFVRWNAMAMVVNANREHSGLGG
                     HISTYASAATLWEVGFNHFFRGRTEKFMGDMVFFQGHAAPGVYARSFLEGRLDERHLH
                     NFRQELAEGGGLSSYPHPYLMPDYWQFPTVSMGLGPLLSIYQARFNRYLRARGIVEGE
                     EPKVWAFIGDGETDQPETLGSLTLAAREGLDNLIWVVNCNLQRLDGPVRGNGKIIQEL
                     EAAFQGAGWKVIKVIWGSDWDKLLAADKTGLLLKRMEEAVDGDYQKFSVEPGSYTRRH
                     FFGKYPELLELVNHLTDEQIHKLLRGGHDSRKVYAAYKAAMDHKGQPVVILAKTIKGY
                     GLGEAGEGRNITHQQKKLNEKELREFRARFAISIADEEIAEAPFFRPPPESREMRYLL
                     GQRKKLGGVLPRRVVKAQPLAVPQLADLGDLLKGSTRTEVSTTMGLVRLLGALLRNPN
                     IGRQVVPIIPDEARTFGLDALFREIGIYSSKGQLYEPVDIKSLLYYNEQKDGQILEEG
                     ITEAGAMASFVAAGTAYATQGVPMIPFYIYYSMFGFQRIGDLMWLAGDMRAKGFLCGG
                     TSGRTTLNGEGLQHQDGHSLILSSTIPTLLTYDPAFTYELVVIIADGLRRMFVKGEEL
                     FYYLALYNENYEMPPMPDGVAEGILQGLYRFKPGTKPNAPKVHLLGSGPIIREALRAQ
                     AILEERFGVSADVWSATSYKTLRNHALQARRWNMLHPAAPPRKSYLETVLANEQGVFV
                     AVSDNMKIVPDQIAPWVPGGLTTLGTDGFGRSDTRQRLRRFFEVDAELTVIAALYALA
                     ERGQIERAVVARAIKDLGVDPEKAHPEITPA"
     gene            3989..5275
                     /locus_tag="PKI07_12725"
     CDS             3989..5275
                     /locus_tag="PKI07_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOC51948.1"
                     /translation="MEMKLPPLGEGADSGTVVNVFVKPGDTITQGQPVLELENEKAVV
                     NIPASAAGTVTAVFVSAGDKLKVGQRMLALAGAAPAETPAPASAPAPRRSRERLIQTP
                     SPEQVPVPDTDADEPASPPKAGVPPMAAPSIRKLARDLGIDLSRVAGSARGGRILLED
                     VREYIQKLQRLATRPRAGGGAPPPPPAERIDFAKWGPVSRKPLSPLREVIARRMSESW
                     STIPRVTQFDEADITAVMALRKKYAPAYEQQKARLTLTSFALKVVVDTLKKHPLFNAS
                     LDEGAHEIVLKGYYHIGIAVDTEAGLIVPVIRDADKKSLLELSRELEDLAGKARQRKL
                     TAEQLQGGTFTISNQGGIGGGHFTPIVNKPEVAILGLGRGAMKATVREQRIEPRLWLP
                     LGLSYDHRAIDGGAAARFTVDLVQAFENFPEEAVKL"
     gene            5291..6709
                     /gene="lpdA"
                     /locus_tag="PKI07_12730"
     CDS             5291..6709
                     /gene="lpdA"
                     /locus_tag="PKI07_12730"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOC51949.1"
                     /translation="MNPIKTEIVVVGAGPGGYAAAFYAADLGKRVILVEREPRLGGVC
                     LNRGCIPSKALLHAAHAITTARESEHRGIVFGPPQLDLARLRSWKESILTRLAGGIAQ
                     LAKLRGVQVLTGRGYFEDSHTLRVETVQGQQFVHYEHAIIAVGSKPALPRAFDLGNPR
                     VMTSTEALELEDIPENLLVIGGGYIGMELGTVYATFGSKVVLVEALDSILAGADPDLA
                     RPIVNYARKTFKEMRLKTRVSQMSTSGKQIRVVSETDGRKLEELYDRVLVSVGRVPNA
                     DDLGLENTKVSFDEKGFIHVNEKQQTTDAAIYAIGDIVGGVLLAHKATKEGRIAVEVI
                     AGEKSAFVDVTIPAVVFTDPEIAWCGLTEAEARAKGLEVQVSKFPWSASGRALTFDRP
                     EGLTKLIIDPDTERILGVGIVGQGAGELLAEGLLAVEMGATAKDLALVVHPHPTMSET
                     LMEAAEAYYGHATHTLLRKKAD"
BASE COUNT         1276 a         1965 c         2395 g         1258 t
ORIGIN      
        1 cgcaaggagc cctccagccc ccccaatacg gcctttattc ctgcctttcc acccctgctt
       61 tccgacctcg ttcacacggt tttccgcccg actgggaaat agtcccctat ttcttcgctt
      121 aaaacccatt ctttatcccg gctttctact ataactactt cattttacag gacttttaca
      181 atacggccta tccctgcccc tcacccttat ctccctctgc cagcaggctg tgtacaaggg
      241 gtgtacaagg gatgctcatc gggtgtaccc gctaaaatcc catgaactcc cggtgggcat
      301 cccttgtaca ccccttgtac acacggtgga gagggcggaa aataaggggt tttcgagggg
      361 ggcggaggga gctaaacgcg gaagttccac tcgcggagcg agtatttttc ggcgacgaga
      421 cggtggatgt ggtcccgggg gatagccgtg aggggggtgg cggctttcca ggctttggcg
      481 agggcggttt tgacggcgtc ttcggagacg ttgaggttga gcaggaaatc gccatgaacg
      541 cggttgaggc gataatccgg ctggagggag ggcggaggga gagtcttttc gaccagggcg
      601 aggtccaggc gcaggaggaa cgagccgtgg aaaagcagga accggcggcg gcggcgctgg
      661 gcgttgccgg cgaacttgag ttcgtcaatg gccaggtcgg tctggccccg ccatgcgatg
      721 ggcgccgcaa ggacgggggc cagggcgtcg cggtggcgtt cgagtataaa ctggttggtg
      781 gcggcgatgc tctggcaggg ctggttgccg gcgatgcgca ggatcagggc gtaattgagc
      841 acgcccgggc cttgcagcac cgtgccgcct ccggtgcacc ggcgcagcac cgggatgccc
      901 tgttcgcggc agaagggaag gttggcctcg gtggcggctt tgttgccgta gccgaggacg
      961 acgaagtaat gccggggcga ccagaaacgg agcacctcgc cgcagccgcc ggcctcgcac
     1021 aggtcgagaa gggcctcgtc ggcggcgagg ttttcctcgg gcgtcggcag cgtaaggtcg
     1081 caaagtttca tgagcttgca gttatcggcc gccatattta gctgacaacc ggcggaaagt
     1141 atgctgtaat tagatttaag ctaaggcgtt tgacgcgcag ccagagagag tataggtaaa
     1201 taacttgtta ttaataaagt gaaagtgccc aagacagaaa agaaaggttc cgccgccagc
     1261 cagccggcgc ctccggtgga ttcacgctcc gaggaggaaa actggctgga ttcgctggag
     1321 acggtgctcg agttcgcgtt gaaagatttg aagccgcagc agacgagccg gtttttggac
     1381 gggttgaccg agcgcttgcg ggcgcggggc aaggaagtgc cgtatgtggt cggcacgcct
     1441 tacattaaca cgatccccga ggagaaagag gccccgtttc ccggcgattg ggaaatggag
     1501 cggcgcatca agagcttcgt ccggtggaat gcgatggcga tggtggtcaa cgcgaaccgg
     1561 gagcatagcg gcctgggggg gcatatttcg acctatgcct cggcggcgac gctttgggag
     1621 gtgggcttta accatttctt ccgcgggcgc acggagaagt tcatgggcga catggtgttc
     1681 tttcaggggc atgcggcgcc gggggtttac gcgcgatcgt tcctggaagg acggcttgac
     1741 gaacggcatt tgcacaactt ccgccaggaa ctggccgagg ggggcggttt atcctcttac
     1801 ccgcatccgt atttgatgcc ggattactgg cagtttccga cggtctccat ggggctgggg
     1861 ccgttgcttt cgatttacca ggcgcgcttt aaccggtatt tgcgcgcgcg gggcattgtc
     1921 gagggcgagg agcccaaggt gtgggcgttc atcggggacg gcgaaactga tcaaccggag
     1981 acgctgggat cgctgacgct ggcggcgcgg gaggggctcg ataatctcat ctgggtggtc
     2041 aactgcaatc tgcagcggct ggacgggccg gtgcgcggga acgggaagat catccaggag
     2101 ctggaggcgg cgttccaggg ggcgggctgg aaggtcatca aggtcatctg gggctcggac
     2161 tgggacaagc tgctggcggc ggacaagacg ggtttgctgc tcaagcggat ggaagaggcg
     2221 gtggacggcg attaccagaa gttttcggtc gagccgggca gctacacgcg gcggcacttt
     2281 ttcggcaagt acccggagtt gctggagctg gtcaaccacc tgaccgatga gcagattcac
     2341 aagctgctgc gcggggggca tgattcgcgc aaggtctatg ccgcctataa agccgccatg
     2401 gatcataaag gccagccggt ggtgatcctg gccaagacca tcaagggcta cgggctgggc
     2461 gaagccggcg aaggccgcaa tatcacgcac cagcagaaga agttgaacga gaaggaattg
     2521 cgcgagttcc gggcgcggtt cgccatctcc attgcggatg aagagatcgc ggaggcgccc
     2581 ttcttccggc cgccgccgga gagccgggag atgcggtatc tgctggggca gcgcaaaaag
     2641 ctgggcgggg tgctgccgcg gcgcgtcgtc aaagcccagc cgctggcggt gccgcagctt
     2701 gcggatctgg gcgatttgct gaaaggctcg acgcgcaccg aggtttccac gaccatgggg
     2761 ctggtgcgct tgctgggcgc gctcctgcgc aatccgaaca tcggccggca ggtcgtgccg
     2821 attattcccg acgaggcgcg gacgtttggc ctggatgccc tgttccgcga gattgggatt
     2881 tactcctcca aggggcagct ttacgagccg gtggacatca agtcgctgct ctactacaac
     2941 gagcagaagg acggccagat cctcgaggaa ggcatcaccg aggccggcgc gatggcctcc
     3001 tttgtggcgg ccggcaccgc ctacgccacc cagggcgtgc cgatgattcc cttttatatt
     3061 tattactcga tgttcggctt tcagcgcatc ggcgacctga tgtggctggc gggggatatg
     3121 cgggcgaagg gctttctatg cggcggcacc tcggggcgca ccacgctcaa tggcgagggg
     3181 cttcagcacc aggacggcca cagcctgatt ctgtcgagca cgatcccgac gttgctgact
     3241 tacgatccgg cgttcaccta cgagctggtg gtgattatcg cggatggcct gcggcgcatg
     3301 tttgtcaaag gcgaggagct gttctactac ctggcgctgt ataacgaaaa ctacgagatg
     3361 ccgcccatgc ccgacggggt cgcggaggga atcctgcagg ggctttaccg cttcaagccc
     3421 gggacgaagc cgaacgcgcc caaggtgcat ctcctgggca gcggcccgat catccgcgag
     3481 gcgctgcggg cgcaggcaat cctggaagag cggtttgggg tgtcggcgga tgtctggagc
     3541 gccaccagct acaagaccct ccggaaccac gcgctgcaag cccggcgctg gaacatgctg
     3601 catccggcgg cgccgccgcg gaagtcttat ttggaaacgg tgctggccaa cgagcaaggg
     3661 gtttttgtgg cggtctcgga caatatgaag atcgtgcccg accagattgc gccgtgggtg
     3721 ccgggggggt tgacgaccct gggcacggac ggttttggcc gcagcgacac gcggcagcgg
     3781 ctgcggcggt ttttcgaggt ggatgccgag ctgacggtca ttgccgcctt atacgcgctg
     3841 gccgagcggg gccagattga gcgggcggtc gtggcccggg ccatcaagga tctgggggtg
     3901 gacccggaaa aggcgcaccc ggagatcacc ccggcgtagc gcgccggcgt ttctttcatg
     3961 cgggctgaac tcaactcttt atagacttat ggaaatgaaa cttcctcccc tgggcgaagg
     4021 cgcggattcc ggcaccgtgg tcaacgtatt tgtcaaaccg ggtgatacga tcacccaagg
     4081 ccagcccgtg ctcgaactgg agaatgaaaa ggcggtcgtc aacatccccg ccagcgcggc
     4141 cgggacggtg acggcggttt ttgtcagcgc gggcgacaag ctcaaagttg gccagcggat
     4201 gctggcgctg gccggagccg ccccggccga aaccccggcc ccggcttccg ccccggcgcc
     4261 ccggcgcagc cgcgagcgcc tgatccagac gccttcgccg gaacaggtgc cggttcccga
     4321 cacggacgcc gacgaaccgg cctccccgcc gaaggccggc gtgccgccga tggcggcgcc
     4381 gtccatccgc aaactggcgc gggatctggg cattgacttg tcccgcgtgg ccggcagcgc
     4441 gcgcggcggg cggattttgc tggaggacgt gcgcgaatac attcaaaagc tgcagcggct
     4501 tgccacccgg ccgcgggcgg gcggcggcgc gccgcctccc ccgccggccg agcggataga
     4561 ttttgcgaag tgggggccgg tttcgcgcaa accgctttcc cccctccggg aggtcattgc
     4621 ccggcgcatg agcgaaagct ggagcaccat tccgcgcgtc acccaatttg acgaggcgga
     4681 cattaccgcc gtgatggcgt tgcggaagaa gtatgccccg gcctacgagc agcagaaagc
     4741 ccggctcacc ctgacctcgt ttgcgctcaa ggtggtggtg gacacgctca agaagcatcc
     4801 cctcttcaat gccagcctgg acgagggcgc gcacgagatc gtgctcaagg gatactacca
     4861 tatcgggatc gccgtggaca ccgaggcggg cctgattgtg ccggtgatcc gggatgcgga
     4921 caagaagagc ttgctggagc tgtcgcggga gctggaagac ctggccggca aggcgcgcca
     4981 gcgcaaattg accgcggagc agctccaagg cggcaccttt accatctcga accagggcgg
     5041 catcggcggc gggcatttta cgccgattgt gaacaagccg gaggtggcca ttctcggcct
     5101 gggccgcggg gcgatgaaag ccacggtgcg cgaacagcgg atcgagcccc ggctgtggct
     5161 gcccctcggg ctttcctacg accaccgcgc cattgatggc ggggcggccg cgcggttcac
     5221 cgtggacctc gtgcaggcct ttgagaactt cccggaagaa gcggtgaaac tatagaagga
     5281 ctgagtcatt atgaatccca tcaagacaga aatcgtggtt gtcggcgccg gccccggtgg
     5341 ctatgccgcg gctttctatg cggcggacct gggcaagcgc gttattttgg tagagcgcga
     5401 gccccgtttg ggcggggtct gtttgaaccg cggctgcatt ccctccaaag ccctgctgca
     5461 cgcggcccac gcgattacca ccgcgcggga atccgagcac cggggcattg tgtttgggcc
     5521 gccgcagttg gacctggccc ggctgcggag ctggaaggaa tccattttaa cccggctggc
     5581 gggcggcatc gcgcaactgg cgaagctgcg cggcgtgcag gtcttaacgg ggcggggtta
     5641 ttttgaggac tcgcacaccc tgcgcgtgga gaccgtgcaa gggcagcaat tcgtccacta
     5701 cgagcatgcg atcatcgcgg tcggctccaa gcccgccctg ccgcgcgctt tcgacctggg
     5761 caacccgcgg gtgatgacct cgacggaggc cctggagctg gaggatattc cggagaatct
     5821 gctggtgatt ggcggcggtt atatcggcat ggagctcggg acggtctatg ccacgttcgg
     5881 gagcaaggtg gttctggtcg aggcgttgga cagcatattg gcgggcgccg atcccgacct
     5941 ggcccggccc atcgtgaact atgcacgcaa gacgtttaag gaaatgcgcc tcaagacccg
     6001 cgtcagtcag atgtccacca gcggcaagca gattcgggtg gtgtcggaaa ccgatggccg
     6061 gaagctcgag gaactctacg accgggttct ggtatcggtc ggccgggtgc ccaacgcgga
     6121 cgacctgggc ctggaaaaca ccaaggtttc cttcgacgag aagggcttta tccatgttaa
     6181 tgagaagcag cagaccaccg atgccgccat ttacgccatc ggcgatattg tgggcggggt
     6241 cttgctggcg cacaaagcga cgaaggaagg ccgcattgcc gtcgaggtta ttgccgggga
     6301 aaagagcgcg tttgtggacg tcaccatccc ggcggtggtt ttcaccgatc cggaaatcgc
     6361 ctggtgcggg ctgacggaag cggaggcgcg cgccaaggga ctcgaggtgc aggtgagcaa
     6421 gttcccctgg tccgcttccg ggcgggcttt gacctttgac cggcccgagg gattgaccaa
     6481 gctgattatt gaccccgata cggagcgcat tctgggcgtc ggcatcgtgg gccagggcgc
     6541 cggggaactg ctggcggaag gcttgctggc ggttgaaatg ggggccacgg ccaaagacct
     6601 ggccctggtc gttcatccgc accccaccat gtcggagacg ttaatggagg cggccgaggc
     6661 ctattacggc cacgcgaccc ataccctgct gcgcaagaaa gccgactgac ccgccgcgcg
     6721 cgggcgaacg ccggggagtt ctccgcggcc ggccggaaaa tcgggcaaac ttggggcttg
     6781 aactcaaagc ccggcgtatt agtatctggg ctggactggg ggtttgccgc cagtcgaagg
     6841 gttcggggcg tagcgtagct tggtagcgcg tctgaatggg gttcagaagg tcgt
//
LOCUS       DAOPQG010000146         6889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_12143_length_6889_cov_13.774949, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000146 DAOPQG010000000
VERSION     DAOPQG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12143_length_6889_cov_13.774949"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1906
                     /locus_tag="PKI07_12735"
     CDS             347..1906
                     /locus_tag="PKI07_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51950.1"
                     /translation="MTDNKVRCVYPRLQLAYLLQFAIWGSWCVALGGYLNGKMSGPHI
                     GYLYMAIPWGAIIAPMFIGPIADRYFSAQKMLGLLHLISGAALVASGVICAQAEAGAA
                     AGTVSIPFGPLMALMLISGICFMPSIPLINTVVFKHIPDSAKAPKVFIFGTAGWIIVN
                     LVVEVFGGGAARPNFFFIGGGCGLFLGLYSFTLPDTPPKGAPAKGARTDTFGLGALSL
                     LKSPTFSVFILCAFMASIFGSNYFFPSVVPYLSEYGYPAPVALGTLNQFSELFFMAIL
                     PFCVARIGLKWVLVIGMGAWAARYFIFTLAGFQFALIGLLFHGMGYAFLYVAAYMFGD
                     RVAPAHLKASVQSLLAFLLLGVGQVLSGYAFGYQFDRNAPAITKTVAVEEGQPGKALP
                     AWNDPAMETSAWRYLDLTKSVKYLCGDKNVFNFGEHLGMYTQPDGSIDLDKLPENWEA
                     GGVDYTRAELKATFERIGTKVTREDYLKAQRHNWKGFFIPAAIFVLVWTLLFSVFGRQ
                     PAAAPAEPAKS"
     gene            complement(2382..3260)
                     /gene="dapA"
                     /locus_tag="PKI07_12740"
     CDS             complement(2382..3260)
                     /gene="dapA"
                     /locus_tag="PKI07_12740"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOC51951.1"
                     /translation="MFAGTYTALVTPFRNGAIDELALERLVRLQVQAGVDGIVPVGTT
                     GESPTVDYNEHVHLIALAVKFARRQIKVLAGTGGNSTSEAVFLTEQAERAGADGSLQV
                     APYYNKPTPEGLFQHFRQVARSTRLPLMLYSIPGRCGVEIGVDTTRRLARECRNIIGI
                     KEAGGHADRVSQLRAALGPRFVILSGDDSLTLPFMAVGAQGVISVASNVIPRQVARMV
                     RAFAAGKTETALKLHQRYYPLFKDLFLETNPIPVKAALAMLGLIKEEYRLPLVPLPPR
                     KRRVLQATMKAVGVLK"
     gene            complement(3262..4050)
                     /gene="dapF"
                     /locus_tag="PKI07_12745"
     CDS             complement(3262..4050)
                     /gene="dapF"
                     /locus_tag="PKI07_12745"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOC51952.1"
                     /translation="MNGAGNDFVLVDNRAQKVRLTRDQIVRLCDRHRGVGADGVILLG
                     PSPSGQADWGWEFYNRDGSSGEMCGNGARCFARFAQRLTEPPGGLTFETGAGIITASF
                     QGERVTVNLTRPRDLRLNEPVPLSTGTETIHSINTGVPHAVIFVPDTDRVAMAQLGPE
                     IRQHPHFGPQGTNVDFAQRLGPNHLRVRTFERGVEGETLACGTGVTAVALIAARRYGL
                     SSPVRIQVRSGGELEVSFKDEGGEFADVRLSGPAEFVFEGRIEL"
     gene            complement(4134..5429)
                     /locus_tag="PKI07_12750"
     CDS             complement(4134..5429)
                     /locus_tag="PKI07_12750"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOC51953.1"
                     /translation="MKPSPAAPASAAILQTLPHRASPGLSGVPSAGAPGRLVSLDAFR
                     GLVILAMTLVNYLAGVKNIPAWAKHLPPEQEGYTFVDVVFPGFLFIVGVAIPLALHKR
                     MARGESGFSLLRRVLTRSAALLFVGVIMVNSSYYSADATGLSKSLWFLLAMLAVVALW
                     NSYPAAAPRLKQRIFLGLRALAALVLAGLLIAFRGQNAEGQVVWLQHAWWGILGLIGW
                     AYLVCCLAYLAFRGSSVALMGMLGFLIALYIGDKHGVLDWLGPIRGFVRVGPVLGSTA
                     ANVMIGVLVGNSFVGEAAALRPAVRVRFLLGFGLGLYLAGTLLRPLHGINKIAATDSY
                     TLVTGGLCCLAFLLVYCLMDLGHLQRWARPLVPIGQNALLAYILPGILANFFTVLGLP
                     GLLWQYHSGWPGALNAGVLTLLIMVITWSATRAGILLRL"
     gene            6103..>6889
                     /locus_tag="PKI07_12755"
     CDS             6103..>6889
                     /locus_tag="PKI07_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOC51954.1"
                     /translation="MAEKDDYLADILVDLGYVSAEQVDEARREAAGAGVGVVDLMLAN
                     KVIRPADVTQAKASHFGAEVVNLSELQIADDVIAAVPRHIARKYRVVPVFKHDNSLTV
                     AVTDPSDLDTIDSLTHLLHAEISLRVASDEDIEAALSKYYAERGGGGLAADPRLKQVI
                     EDLTREHVEIQAVDAGDGAVVEADAPLIKLVNTIIVDAFKMRASDIHLEPLAKTFRMR
                     YRIDGVLHEVKAPPRKLQAAVISRLKIQSNMSISEHRIPQDGRI"
BASE COUNT         1285 a         2053 c         2282 g         1269 t
ORIGIN      
        1 tttcaggccc ataaatagcc accaactagc cgtgtacaag gggtgtacaa gggatgctca
       61 ccgggagaac aacagaaaat ccgatgggca tccggggtgc atcccttgta caccccttgt
      121 acacggctgg aagggatggg ggcaggaggg gatagggaag aggtagggat gccgttattg
      181 ggaagagatt tgggaatgga attccggctg gtactgaagg ctgggcgatt gcggcgagtt
      241 tttctcttga gccggggcaa ggttcctgcc ataagaagcg cattgtggat ggcccttctt
      301 tcgagggcga tcaatggcat caaccgaaaa cgatcaggag tatagaatga cagacaacaa
      361 agtgcgctgt gtttacccga ggctgcagtt ggcctatctg ctgcaatttg cgatttgggg
      421 gagttggtgt gtggccttgg gcggctattt gaacgggaag atgagcggcc cgcatattgg
      481 ctacctgtat atggcgattc cgtggggggc gattattgcg ccgatgttta tcgggccgat
      541 tgcggatcgg tatttctccg ctcaaaagat gctggggctt ctgcacctga tctcaggggc
      601 ggcgctggtg gcgagcggcg tgatttgcgc gcaggccgag gcgggagcgg cggctggcac
      661 ggtctcgatc cccttcgggc cactgatggc gttgatgttg atttccggga tctgctttat
      721 gccgtccatc ccgctgatta acacggtggt gttcaagcac ataccggatt cggccaaggc
      781 gcccaaggtc tttatctttg ggacggccgg ctggattatc gtgaacctgg tcgtggaagt
      841 cttcggggga ggggcggctc gtcctaattt ctttttcatc ggtggcggct gcgggctatt
      901 ccttgggctt tactcattta cattgccgga tactccgccc aagggggcgc cggcgaaagg
      961 agcccggacg gatactttcg gcttgggggc cttgtcgttg ttgaagtccc ccacgtttag
     1021 cgtcttcata ctttgcgcgt tcatggcgag cattttcggg agcaactact ttttcccgtc
     1081 ggtggtgccc tatctgagcg agtatggtta cccggcgccg gtggcactgg gcacgctcaa
     1141 ccagttttcc gagctgtttt tcatggcaat cctgccgttt tgcgtcgccc ggatagggct
     1201 caagtgggtg ctggtgattg gcatgggtgc ctgggcggcc cgctatttca tctttacgct
     1261 ggcggggttc cagtttgcgc tgatcgggtt gctcttccac gggatggggt atgcgttcct
     1321 ttatgtggcg gcctatatgt ttggcgaccg ggtggccccg gcgcatctca aggcgagcgt
     1381 ccagagtttg ctggcgtttt tgctgttggg agtcggccag gtgctcagcg gttacgcgtt
     1441 cggctaccag tttgaccgca atgccccggc catcaccaag acggtggcgg tcgaggaagg
     1501 gcagccgggg aaagcgctcc cggcatggaa tgacccggcg atggaaacct cggcttggcg
     1561 ttatttggat ctgaccaagt cggtcaaata tctctgtggc gataagaacg tattcaactt
     1621 cggggagcac ctcgggatgt acacccagcc ggacggttcc attgacctgg ataagctgcc
     1681 ggaaaactgg gaagcgggcg gggtggacta cacccgggca gagttgaagg cgacgttcga
     1741 gcggattggg accaaggtca cccgcgagga ctatttgaaa gcccagcggc acaactggaa
     1801 ggggttcttc attccggcag ccattttcgt gctcgtgtgg acgctcttgt tcagtgtgtt
     1861 tggccggcag ccggcggcgg cgccggctga gcccgcgaag agctaagcgg gagccgggcc
     1921 ccgaggggcg cggcatcgag cgccacggct ggcggcgagg gttcgtcgcg cgccggcgcg
     1981 gcggctttgg tgaaaggccg caaatgcgag ggtgcgtgat gggggctgct gccggctggc
     2041 cgcctttggc gcggactgct gctggggtgg cagggatgtc gtgggctgca gtccagtagc
     2101 gccccgccga gggcggcggg gtactgtcgg caaggtttct aaaggggcgg ccttgcgatg
     2161 gcgaggcgtc cctaaccggg gatttatccg cctccgcgtc ccaaacagcg gccggctatc
     2221 cgcgcacgcc cgcattgtcg ccgcgcaggc ggcagttctt ccaaacaaac cgcttggggg
     2281 gcgggctcta aacgggggtc tgctctatcc ggtttggagc gtggggtgcg gggtgcggcc
     2341 tcacgcacgg cgcgacctcg gatcgcaggc ggccttgttg cctacttcaa cactcccact
     2401 gccttcatcg tcgcctgcag aacccgccgt tttcgcgggg gcaagggcac gaggggcagg
     2461 cggtattcct ctttgatcag gccgagcatc gccagggccg cctttacggg aatcgggttg
     2521 gtctcgagga acaggtcttt gaaaagcggg tagtatcgtt ggtgcagctt gagggccgtt
     2581 tcggttttgc cggcggcaaa cgcgcgcacc atgcgcgcaa cctggcgcgg gatgacattt
     2641 gaggccacgc tgatgacgcc ctgcgcgccc accgccatga acggcagggt gagcgaatcg
     2701 tcgccgctga gaatcacgaa gcgcggcccg agcgcggccc gcaactggct cacgcggtcc
     2761 gcgtggccgc ccgcttcctt gatgccgatg atgttccggc attcgcgcgc cagccgccgg
     2821 gtggtgtcca cgccgatttc gacgccgcag cggccgggga tgctgtagag catgaggggc
     2881 aggcgcgtgc tccgcgcgac ctggcggaag tgctggaaca ggccttcggg cgtgggtttg
     2941 ttgtagtagg gcgccacttg gagggagccg tcggccccgg ctcgctccgc ctgttccgtc
     3001 aaaaagaccg cttcgctggt ggaattgcct cccgtgcccg cgagcacttt aatttgccgc
     3061 cgcgcaaact ttacggccag cgcaatgagg tggacgtgct cgttgtaatc caccgtcggc
     3121 gattcgccgg tggtgcccac ggggacgatt ccgtccacgc ccgcctgcac ctggagtcgg
     3181 accagccgtt ccagcgccag ctcgtcaatc gcgccgttcc ggaacggcgt caccagggcg
     3241 gtgtaggttc cggcaaacat cctaaagctc aatccttcct tcgaagacga attccgcggg
     3301 gccgctcagc cgcacgtcgg caaattcccc gccttcatcc ttgaagctga cttccaattc
     3361 cccgccgctg cggacctgga ttctgacggg ggacgagagg ccgtatcgcc gcgccgcaat
     3421 cagcgccacg gcggtcaccc cggtgccgca cgccagcgtt tcgccctcca ctccgcgctc
     3481 gaaggttcgc acccggaggt ggttgggccc cagccgttgc gcaaagtcca cgttggtgcc
     3541 ctgggggccg aagtgcgggt gctggcggat ttccggcccg agctgcgcca tcgccactcg
     3601 atcggtatcc ggcacgaaga ttaccgcgtg cggcactccc gtattgatcg aatgaatcgt
     3661 ttccgtgccc gtggaaagcg gcaccggttc attaagccgc agatcccggg ggcgggtcag
     3721 gttgacggtg acgcgctcgc cctgaaagct cgcggtgatg atgcccgctc cggtctcgaa
     3781 ggtgagcccg cccggcggct cggtcagccg ttgcgcgaac cgggcaaaac agcgcgcgcc
     3841 gttgccgcac atctcgccgg agcttccatc gcggttatag aactcccagc cccagtccgc
     3901 ctggcccgag ggggaggggc ccaggagtat gacgccgtcc gcgcccactc cccgatgccg
     3961 gtcgcagagg cgcacgattt gatcgcgggt gagccggact ttctgtgccc ggttgtcaac
     4021 cagcacgaag tcgtttcccg cgccgttcat tttggtgaag tccagaacca ttaagcggaa
     4081 ggctaaggcg tggcggggcg agtgtcgaga cgggagtcgt gccgatggcg gcatcacagc
     4141 ctaagcagga tgccggcgcg ggtcgcgctc cacgtgatga ccatgatcag aagggtcagg
     4201 acgccggcgt tcagggcgcc gggccagccg ctatggtatt gccacagcaa gcccggcagg
     4261 cccagcacgg taaagaagtt ggcgagaatg ccaggcagaa tgtaggccag cagcgcgttc
     4321 tggccgatgg gcaccagcgg cctggcccag cgctgcaggt ggcccaggtc cataaggcaa
     4381 tacacgagca aaaacgccag gcagcaaagc ccgccggtga ccagggtgta ggaatccgtc
     4441 gcggcgattt tattgatgcc atgcagcggg cggagcaggg tgcccgcgag gtagagcccc
     4501 agcccaaacc ccagcaggaa ccgaacccgg acggcgggcc ggagcgcggc tgcttccccc
     4561 acgaaactat ttccgaccag cacgccaatc atgacattgg cagccgtgct gcccagaacc
     4621 ggcccgaccc tgacgaagcc gcggatgggg cccagccaat cgagcacgcc gtgcttgtcg
     4681 ccgatgtaaa gcgcgatcag aaagccgagc atccccatga gcgcaacgga gctgccacga
     4741 aatgccaggt aggccaggca gcagacgagg taggcccagc caatcaggcc cagaatgccc
     4801 caccaggcat gttgcagcca gacaacctgc ccctccgcat tctggccgcg gaaagcaatc
     4861 agcaaacctg ccagcacgag ggcggccagg gcgcgcagcc ccaggaaaat ccgctgtttc
     4921 aggcgcggcg cggcggcagg ataactattc cacagcgcca cgaccgccag catggccagc
     4981 aaaaaccaaa ggcttttgct caggccggtg gcgtcggccg agtaatagct cgaattaacc
     5041 atgatgaccc cgacgaacag cagcgcggcg gagcgcgtaa gcacgcgccg cagcagagag
     5101 aatccggatt caccccgggc catgcgctta tgcagcgcca atggaattgc gacgccgacg
     5161 atgaacagaa accctggaaa caccacatct acgaacgtat agccttcctg ttccgggggc
     5221 aggtgtttgg cccacgcggg gatgtttttc accccggcga gatagttgac gagcgtcatt
     5281 gccaggatca ccaggccgcg gaaggcatcc agcgagacca ggcggccggg cgcgcccgcg
     5341 gacgggacgc cggagaggcc cggggatgcg cggtgcggca aagtctgcag gatggcggca
     5401 gaagcggggg cagccggcga cggtttcatg cttcgcaatc tgaaccaatc gcgggccggc
     5461 gacaagcaga agccgccggc gggcggatgc tctgttctgc ccggcgggcg ggcgccccgg
     5521 atactccaag cccgggcgca actccaagga gtcacgcccg ggcttattcc caggacaggg
     5581 cagctttcat accttacccc gccgcccctg gtctttaatc cccacaaaca ccaccaactc
     5641 tcgcgcggta tccagaaccg caagagtaag ttaacgcaaa gcccccggga ggtatagtca
     5701 gccaatggcg gattattttg taagggaaat ccttatcgat cgggggccga cgcgctctgc
     5761 ttcctttcca aaaaaaaagc gcagtcagcg aagaccgcgc taagggcggg cggcgcggag
     5821 gtagagcgtt tccggccgcc atcagcctgt tccgtttaac ggaatacaac actatccttt
     5881 ctcgttggag aggccgcaat gccaataaat acgcaagcaa ccgctgtgta gttctacgga
     5941 taaaagcgcc gcggcgggaa caggccgggg gcagcctccc agccaggggg ggggcgcacc
     6001 tgatggccag attttggcac cgcgcggatt gacattaccg attgggttag tatgctgcta
     6061 cacaagtttc taacgacatt cgtgcgtagc ataaaataaa ctgtggctga gaaagacgac
     6121 tatctggcag atatcctcgt ggatttgggg tacgtttctg ccgagcaggt ggacgaggcg
     6181 cgccgcgaag ccgccggggc cggggtgggc gtggtggacc tcatgctcgc caacaaggtt
     6241 atccgtcccg ccgatgtcac gcaggccaag gcctcccatt tcggcgcgga agtcgtcaac
     6301 ctcagtgagt tgcagatcgc cgatgacgtc atcgccgcgg ttccccggca cattgcccgc
     6361 aagtaccggg tggtgccggt ctttaagcac gataacagcc tcaccgtcgc ggtgaccgac
     6421 ccgtcggacc tcgatacgat tgatagcctg acgcacctgt tgcacgccga gattagcctg
     6481 cgcgtcgcct ccgacgagga catcgaggcg gccttgagta aatactacgc cgagcgtggc
     6541 gggggcggcc tcgcggcgga cccgcgcctc aagcaggtga tcgaggacct cacgcgcgag
     6601 catgtggaga tccaggccgt tgatgccggc gacggggcgg tggtggaagc ggatgcgccg
     6661 ctcatcaagc tggtcaatac catcatcgtg gacgcgttca agatgcgcgc gtcggatatt
     6721 cacctggagc cgctggcaaa gaccttccgc atgcgctacc gcattgacgg cgtcctgcac
     6781 gaggtcaaag ccccgccccg gaagctgcaa gccgccgtta tcagccgcct gaaaatccag
     6841 tccaacatgt ccatctccga gcaccggatt ccccaggacg ggcgcatcc
//
LOCUS       DAOPQG010000147         6783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_12382_length_6783_cov_13.835464, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000147 DAOPQG010000000
VERSION     DAOPQG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12382_length_6783_cov_13.835464"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKI07_12760"
     CDS             complement(<1..345)
                     /locus_tag="PKI07_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51955.1"
                     /translation="MIILGNFFLLALSILDRELALQALRTAVAPALEMFIGINVQSQQ
                     AAHLIAHESLVALEHFVGGRQRGPQRLQPHPRGGISQGIIADGLGVAQRVAPGFPARL
                     ALELKKAGGGGGG"
     gene            395..1426
                     /locus_tag="PKI07_12765"
     CDS             395..1426
                     /locus_tag="PKI07_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51956.1"
                     /translation="MANPCVRGGGRAVALRLLGRRRRLYGVRFLDVVVADAGYAKGPF
                     LKAVTALGWALVVVLKQGDYDVYQEAWALTQGQPTHQFRQEEREVRLWEVRSLTFSKT
                     YGRAVRGVQAEKKWTQFKVVGGKKIPVPKQSDWLWGVTEDLGGYGGRTIGNLGHGWWR
                     IENYAFNVLAQHWHLRHCAHHDPTSILACLLITLLPSTCFMPLFCSRANSIGRARSPC
                     KNCACNSTGPLNTARSCSCSAGERKDRLSAVCSLILQNWPADLHTRSVYWWNGRGPLD
                     RLEGTSRPSFPSASAAPIMVETSIRNFCRGGCGVAVRGGLQYARPHDGLHGQAGLAAE
                     LKVHGAAKT"
     gene            1407..2237
                     /gene="cysT"
                     /locus_tag="PKI07_12770"
     CDS             1407..2237
                     /gene="cysT"
                     /locus_tag="PKI07_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOC51957.1"
                     /translation="MARQRHNVLPGFSLTMGFTLFYLGAIVLIPFAALVLRALELSWL
                     DFWRLATTERALAAYRLTFGASLIAAAANGVFGTLLAWVLVRYHFPGRRSLDALIDFP
                     FALPTAVAGLTLASLFAPDGWLGRYLVPLGIKGAFTPLGVVIALTFVGLPFVVRTLQP
                     VLQNLEREVEEAAATLGAGRLRTFAQIIAPTLLPAVLTGLALAFARAVGEYGSIVFIS
                     GNLPFKTEIAPYLIVQRLEEYDYTGAIALAVVLLVFSFLLLGAINLLEQWSRHARRPA
                     "
     gene            2242..3147
                     /gene="cysW"
                     /locus_tag="PKI07_12775"
     CDS             2242..3147
                     /gene="cysW"
                     /locus_tag="PKI07_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOC51958.1"
                     /translation="MTTFFPGKRGTAPRAAQRGVEESSLAKWTLIGIALVFCVGFLLL
                     PLLNVFAQALAKGGAHYWRSLTEPDSRAAIRLTLMVAAICVPLNVLFGLAAAWAIAKF
                     EFRGKPFLVTLIDLPFSVSPVVAGLMLVVLLGQQGFFGEWLAAHEIKIIFAVPGLVLA
                     TVFITFPFVARELIPVMQAAGTEQEEAALTLGASGWQTFWHVTLPSVKWGLIYGVILC
                     NARAMGEFGAVSVVSGHIAGLTETMPLRVQALYEGATTATTAAAFAVASLLALLALLT
                     LGVKSALEWRQARAERIAASGAGQR"
     gene            3144..4247
                     /locus_tag="PKI07_12780"
     CDS             3144..4247
                     /locus_tag="PKI07_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC51959.1"
                     /translation="MSIELQGVTKKFGDTVAVNNVSFSVREGELMALLGPSGGGKTTV
                     LRLVAGLEMPTAGDVFIRGRRVNDLSVQQRNIGFVFQNYALFKNMNVFRNIAFGLKIK
                     KWKRARMQARIEELLKLFGLEGLERRYPHQLSGGQRQRVAIARALAPQPSVLLLDEPF
                     GAVDARIRQELREWLVTLHHDLNVTTVFVTHDQEEALEVANRIVIFSGGVLEQVGTPR
                     EVYDQPANEFVARFIGAMNVLEAKVREGVARIGELEFPAPGEAEGQQVRVGFRPYAVQ
                     VSADLTRYRYHAVLQHTFFLGVLLRLELELPSGLTLRARMTKEDYAQQGLEDGRRVSF
                     QIRQYRLLAGEAAALGPEAELQPETPPVLGDYI"
     gene            4394..5242
                     /locus_tag="PKI07_12785"
     CDS             4394..5242
                     /locus_tag="PKI07_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC51960.1"
                     /translation="MNYNSSSALNRREFIGAVGLTAMVLGTAPWAQGAPGKKIPIGLQ
                     LYSLREQCKTDLPGMLAAVAKIGYRGVEFAGYHGRSAKELRKLLDDHGLVACGTHTPY
                     ESVLPNKLEETVEFNRILGNKFLIVPWMSEAKSKQEWLDRAKLFNEIADKVKAQGMWI
                     GYHAHAHDFKQIDGVSAWDLFFGNTKPTVVMQLDTSNCLAGGADPVAVLNRYPGREQT
                     IHLKAHGGGPDAVIGEDKVDWKSVFAFCERKGKTQWYIVEHESARDPLDAVARCFEAL
                     RKFGKV"
     gene            complement(5307..6266)
                     /locus_tag="PKI07_12790"
     CDS             complement(5307..6266)
                     /locus_tag="PKI07_12790"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOC51961.1"
                     /translation="MKSKMNGCGKMYRRPGSCQIIAVFMVALAPLVANCQPSVPAARV
                     VVITNAPAAKPTDVVAQATLSRWKPIFRGVEICEGATKVPRRLQVRAVRIDLREPTIR
                     FLVTPRIGNGTNAWGGRTTSEFLSEFKCQAALNGSIFDVFAKQRGDPTRVQGLSLSRG
                     DLYQSRHDWDALLISQDRRAWVARSPVDVRGAYNGLSGFHALLINGTNNGTMVSRHPR
                     SAVGISRNLRYLYLVAIDGRQNGYSEGTTTAETAEWMRKLGAYNALNLDGGGSTALVI
                     EGPDGSPELLNRPCGLPVGVERRVANHLGVFAQKLPNSGRGRR"
     gene            6524..6599
                     /locus_tag="PKI07_12795"
     tRNA            6524..6599
                     /locus_tag="PKI07_12795"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6557..6559,aa:Phe,seq:gaa)
BASE COUNT         1220 a         1903 c         2237 g         1423 t
ORIGIN      
        1 cccccccccc ccccccccag ctttcttcaa ctccaaagcg agccgcgccg gaaagcctgg
       61 cgcaacacgc tgggctacgc caagcccatc cgcgatgatg ccctggctta tgccaccgcg
      121 cggatgcggt tggagccgtt gcggaccgcg ctggcgacca ccaacaaagt gctcaagcgc
      181 aacaaggctt tcgtgcgcaa tcagatgagc ggcctgctgg ctctgaacat tgatgccaat
      241 gaacatttca agagccggag ccactgctgt ccgcagtgcc tgcaacgcca gctcacggtc
      301 aagaatgctc agggccagga ggaagaagtt accgagtatt atcacaaaga ggtttatgcc
      361 caactcagcg gaccggcctg gaacacgctg ttggatggcg aacccctgcg tccggggagg
      421 agggcgggcg gtggcgttgc gcttgttggg ccggcggcgg cgtctctatg gcgtgcgctt
      481 cctggatgtg gtcgtggccg atgccgggta cgccaagggg ccgttcctca aagcggtgac
      541 cgctttgggc tgggccctcg tcgtggtgct caagcaaggg gattacgacg tctaccagga
      601 agcctgggcc ttgacccaag gccaacccac ccaccagttc cgacaagagg agcgcgaggt
      661 ccgcctttgg gaagtgcgca gcctgacctt ctccaaaacc tatggccgag ccgtacgggg
      721 ggtgcaggcc gagaagaaat ggacccagtt caaggtcgtg ggcggcaaaa agatacccgt
      781 tcccaaacag tccgactggt tatggggggt gaccgaggac ctcggcggct atggtggccg
      841 gaccattggg aacctggggc acggttggtg gcggatcgag aactacgcct tcaacgtcct
      901 ggcccagcac tggcacctgc ggcactgcgc ccatcatgat ccgacttcga tcctagcctg
      961 tttgctcatt acgctgctgc cctcaacctg tttcatgcct ttgttctgct ctagggcaaa
     1021 ctctatcggc agggcaagat caccctgcaa gaactgcgcc tgcaactcta ccgggccgtt
     1081 gaacacggct cgctcctgct cctgttcagc gggggagcgc aaggataggc tttcggccgt
     1141 ctgttctttg atacttcaaa actggccggc ggaccttcac acaagaagtg tgtactggtg
     1201 gaacgggcgc ggccccctcg accgactcga aggaacctct cgccccagtt ttcccagcgc
     1261 gagtgccgcc ccaatcatgg tggagaccag catacggaat ttctgccggg gcggatgcgg
     1321 ggttgccgtg cgcggcggtt tgcagtatgc tcggccgcac gatggcttac atggacaggc
     1381 cggtctggcg gcggaactaa aggtgcatgg cgcggcaaag acataacgtg ctacctggct
     1441 tcagcctgac gatgggcttt acgcttttct acctgggcgc aattgttctg attccttttg
     1501 ccgccttggt tttgcgcgcg ctggagctgt cgtggctgga tttctggcgt ttggcgacga
     1561 cggaacgggc actggcggcg tatcggctga cgttcggggc atcgctgatt gccgcggcgg
     1621 ccaacggggt gtttggcacg ctgctggcct gggtgcttgt gcgctatcat ttccccggcc
     1681 gccgcagtct ggatgcgctc attgatttcc cgtttgcgct gcccaccgcg gtggcaggat
     1741 tgacgctggc cagcctgttt gcccccgacg gctggctggg gcgttacctg gtgccgctgg
     1801 gaatcaaagg tgcgtttacg ccgctggggg tggtaattgc gctgacgttt gtggggctgc
     1861 cttttgtggt gcggacgctc caaccggtgt tgcagaacct ggagcgggag gtcgaggagg
     1921 cggcggccac gctgggcgcg ggccggttgc gcacgttcgc gcagatcata gccccaacgc
     1981 tgctgccggc ggtgctgacc gggctggcgc tagcgtttgc ccgggccgtc ggggaatacg
     2041 ggtccattgt atttatttcg ggcaaccttc ccttcaagac ggagattgcg ccgtacttga
     2101 tcgtgcagcg cctggaagaa tatgactaca cgggagcgat tgcgctggcg gtggtgctgc
     2161 tggtcttttc gtttctgctg ctcggggcca tcaatttgct ggaacagtgg tcccgccatg
     2221 cgcgccgccc cgcttgaggt tatgacgacc tttttcccag gcaagcgtgg aacggcgccg
     2281 cgcgccgcgc agcgaggcgt ggaggaatcg tcgctggcga agtggacgct tattggcatt
     2341 gccctggttt tctgcgtggg gtttctgctg ctgccgctgc tgaacgtctt tgcgcaggcc
     2401 ctggcgaagg ggggggcgca ttattggcgt tcgctgacgg aaccagattc gcgggcggcc
     2461 attcgcctca cactcatggt ggccgccatc tgcgtgccgc tcaatgtgct gtttggattg
     2521 gcggcggcgt gggccattgc caagttcgag ttccggggca agccgtttct ggtcacgttg
     2581 atagatttgc cgttttcggt ttcgccggtc gtggccgggc tgatgcttgt cgtgctgctg
     2641 gggcagcaag gtttctttgg cgaatggctc gcggcgcatg agattaaaat tatttttgcg
     2701 gtgcccggcc ttgtgctggc gacggtattc atcacctttc ctttcgtcgc ccgggagttg
     2761 attcccgtga tgcaggccgc cggcacggag caggaagaag cggcgctaac gctgggggcc
     2821 agtggctggc agacattttg gcacgtcacg ctgccttcgg tgaagtgggg cctgatctac
     2881 ggggtcatcc tttgcaacgc gcgcgcgatg ggagagtttg gcgccgtctc ggttgtttcc
     2941 gggcacattg ccggactcac ggagacgatg ccgctgcggg tgcaggcgct ttacgagggc
     3001 gcgaccacgg cgaccacggc ggcggcgttt gcggtggcca gcttgctcgc cttgctggcg
     3061 ctgcttaccc tgggggtcaa gagtgccctg gaatggcggc aggcacgggc ggagcggata
     3121 gccgccagcg gggcaggcca acgatgagca tagaactgca aggcgtcacc aagaagttcg
     3181 gcgataccgt ggccgtcaac aacgtcagct tttccgttag ggaaggcgag ttaatggccc
     3241 tgctgggccc gagcggcggg gggaagacca cggtgttgcg gctggtagcg gggctggaaa
     3301 tgccgacggc gggagacgta tttatccgcg gccggcgggt gaatgattta tcggtgcaac
     3361 agcggaacat agggttcgtg tttcagaatt atgccctgtt caagaacatg aacgtgttcc
     3421 ggaatattgc ttttggcctg aagattaaaa agtggaagcg cgcccggatg caggcgcgga
     3481 ttgaggagct gttgaagctc tttgggctgg aggggttgga acggcgctac ccgcaccagt
     3541 tgtcgggcgg ccagcgccag cgggtggcca tcgcccgggc gctggcgccg caaccaagtg
     3601 ttttattgct ggatgagccg ttcggcgctg tggacgcccg aattcgccag gaactgcggg
     3661 aatggctggt gacgctgcat catgatttga acgtgacgac ggtgttcgtc acgcacgatc
     3721 aggaagaagc attggaggtc gccaaccgca tcgtcatctt ttccggcggc gttctggagc
     3781 aggtgggcac cccgcgcgag gtttatgacc agccagcgaa tgagttcgtc gcgcgcttca
     3841 tcggcgcgat gaatgtgctg gaagcgaaag tgcgggaggg cgtggcgcgc atcggcgaac
     3901 tggagttccc cgcccccggc gaggccgagg ggcagcaggt acgagtgggg tttcgtcctt
     3961 atgcggtgca ggtttccgcg gatctgacac gttatcgcta tcacgcggta ttgcagcata
     4021 cgtttttcct gggggtgctg ctgcggctcg aattggagtt gcccagcggg ttgaccctcc
     4081 gcgcccgcat gaccaaggaa gactacgcgc agcaggggct ggaggatggg cggcgcgtct
     4141 ctttccaaat ccgccaatac cgcctgctcg ccggagaggc ggcggcgttg gggccggagg
     4201 ccgagttgca gccagagacg ccgccggtgc tgggagacta tatttgagcg gcagattccg
     4261 ggaggtgccg gcgctttccg ttgcggtttg cgctgccaac agccggcagt tgacgcgagg
     4321 cttcgttccg ggataaggtg tgagcggcaa tggcactatg ccttccatac taaccaaacg
     4381 aatgagatca tctgtgaatt ataattcatc ttccgctttg aatcgccgcg agtttatcgg
     4441 ggcagtcggc cttaccgcca tggttctggg aactgcgccc tgggcccagg gcgccccggg
     4501 caaaaagatt ccaattgggc tgcaactcta ttcgctgcgc gaacagtgca agaccgactt
     4561 gccgggtatg ttggcggccg tagcaaaaat cggatatcgc ggtgtcgaat ttgccgggta
     4621 tcatggccgc agcgcgaagg aattacgcaa gctgcttgac gatcatggcc tggtcgcctg
     4681 cggcacgcat acgccgtacg aatctgtgtt gccgaataag ctggaagaga cggtcgagtt
     4741 caaccgcatc ctgggcaaca agtttctcat cgttccctgg atgagcgagg cgaagtccaa
     4801 gcaggaatgg ctggatcggg cgaaactgtt caacgaaatc gccgacaaag tgaaggccca
     4861 gggcatgtgg atcggctacc atgcgcacgc ccatgacttc aagcagattg atggggtatc
     4921 agcctgggac ctgttctttg gcaacaccaa gcccacggtg gtcatgcagc ttgacaccag
     4981 taattgcctg gccggagggg cggacccagt cgccgtgctg aatcggtatc ccgggcggga
     5041 gcagacgatt cacctcaaag cccacggtgg tggacccgat gccgtcatag gcgaagacaa
     5101 agtggattgg aagtcggttt ttgccttttg cgagcgcaag ggcaaaaccc agtggtatat
     5161 tgtcgagcat gagagtgcgc gggatcccct tgatgcggtc gcgcgctgtt tcgaggcgct
     5221 gcgaaaattc ggcaaagtgt gagctgaaaa gcctggcgca acattgcgtc cgccgtggcc
     5281 cggttaatcg ctcgggcaag ctcccgtcag cgtctcccgc gcccggagtt cggtagtttt
     5341 tgggcgaata cgccgaggtg gttggccact ctgcgctcaa ccccaaccgg taatccgcac
     5401 gggcggttta gcagttccgg agagccgtcc gggccttcga tgaccaatgc ggtgctgccg
     5461 ccgccgtcca ggtttagcgc gttgtaagcc ccgagtttac gcatccattc cgccgtctcc
     5521 gccgtggtgg tgccctcgct gtagccattt tgccggccat caattgcgac aaggtagagg
     5581 tagcgcaaat tacggctgat tcccactgcg gagcgcgggt ggcggctgac catcgtgcca
     5641 ttgttcgtgc cattgatcag cagggcatgg aatccgctca ggccattata ggctcctcgg
     5701 acgtccactg gtgaacgtgc cacccaagcc cgccggtcct ggctgatcag gagcgcgtcc
     5761 caatcatgcc gggattgata gaggtctccg cgagacaatg acaggccctg cacgcgggtc
     5821 ggatcacccc gctgtttggc aaatacgtca aagattgagc cattgagcgc ggcttggcat
     5881 ttaaactcac ttagaaactc ggaagtggta cggcctcccc aggcgttggt gccgttgccg
     5941 atccgcggtg tgaccagaaa acggatggtc ggttcacgca gatctatccg aactgcccga
     6001 acttgcaggc gtcgaggaac cttggtcgcg ccctcgcata tttccacgcc ccgaaagatc
     6061 ggtttccagc gcgaaagcgt ggcttgagcg acgacatcgg tcggttttgc ggccggggca
     6121 ttggtaatta cgaccactcg cgcggctggc acggaaggtt ggcaattcgc cactagcggc
     6181 gcgagcgcga ccataaaaac ggcgattatc tggcagctcc ccggtcgccg atacatcttg
     6241 ccgcagccat tcattttgga tttcatcggc ggcaacccta acattccgct cagagaaata
     6301 gcaacggcag gttcgccatg ctcatctttg cttttttcgg ggcttgcgct tgggctggga
     6361 attgtggcac agtggcagcg tgttaagacg gaagtgagtc ttgcgcctgt tgagcgtgcc
     6421 tgcgtgcgag ggagccgggg cactgcccaa ggccacaatg tggtcgggtc atcggggagc
     6481 agcgtacggg gttcgctgcg cggactgaac gggcggcggg gcaggctggg tagctcagtt
     6541 ggtagagcag aggactgaaa atccttgtgt cggcggttcg attccgcccc cagccaccac
     6601 cgcaattcct ccgtggcaat caagggtttc tgaacatcgg ccatcctccg tatcagaatt
     6661 tgcccgcgga ctaaaattta ggcgggctca gttgaaataa aagagccgcg gttttacgtt
     6721 gagggaggta ttgcctttcc aaaagcggag acatttttat ttcgctgtct ccacctgcag
     6781 atc
//
LOCUS       DAOPQG010000148         6777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_12401_length_6777_cov_14.630765, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000148 DAOPQG010000000
VERSION     DAOPQG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12401_length_6777_cov_14.630765"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..865)
                     /gene="amrB"
                     /locus_tag="PKI07_12800"
     CDS             complement(50..865)
                     /gene="amrB"
                     /locus_tag="PKI07_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018877605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOC51962.1"
                     /translation="MIRADTSSTGKIRPPAFAGRFYPADPAELAGLVRTLLAQAPPAD
                     GPAPKALIVPHAGYIYSGPIAASAYAGFIPARQLIQRIILLGPSHRVALDGLATSSAR
                     AFASPLGQVPLDGETTRRVRALPQVREQDEAHAGEHSLEVQLPFLQTVLGGFTLLPLA
                     VGDAAPEDVAEVLETVWGGPETRIVISSDLSHYQDQQTAHRLDRATARAIETLNPEGI
                     GDDRACGRIPIRGLLQIARQRHLRARTLDLRTSGDTAGPRDHVVGYGAFAFLE"
     gene            complement(866..2071)
                     /gene="amrS"
                     /locus_tag="PKI07_12805"
     CDS             complement(866..2071)
                     /gene="amrS"
                     /locus_tag="PKI07_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOC51963.1"
                     /translation="MPNVTAQTGPGPGSASPGTPATTRPPRETLADVLARHTTVGALA
                     RSEGGRIRCVACGHRCLIAPGRTGVCRVRFSRDGQLRVPFGYVAGVQCDPVEKKPFYH
                     VYPGSGALTFGMLGCNFHCSFCQNWVTSQALRDAAAGAPWQPVTPRQLVSAGERAGAR
                     LIVSSYNEPLITAEWAAAIFQEGKAAGLDGAFVSNGCATPEVLDFLRPWLAACKIDLK
                     GFDEKRYRTLGGTLGQVTEGIRLVHQRGLWLEVVTLIVPGFNDQEAELRQLARFLASV
                     SRDIPWHVTAFHPDYKLTGPRATSTAQLIRAAEIGAAEGLRFVYAGNAPGQVGPWENT
                     RCPHCHATLIERRGFRVREYRITPGGGCPDCGTAIPGLWSADKTGKTLAGPASSPRNR
                     IFPRGLKGN"
     gene            complement(2195..3628)
                     /locus_tag="PKI07_12810"
     CDS             complement(2195..3628)
                     /locus_tag="PKI07_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC51964.1"
                     /translation="MDNIERPGPTAARADRQPGKGRRAPWGLGALADFFKTGADQPLL
                     ADAEEIKRLYRRGRISVFLSITLGYALYYVVRQGFAVVKKPLLDGGVFNPEQMGAIGS
                     VFFFTYMIGKCFNGFLADRLNIKRFLAAGLFLSAATLVVMGCVTGFYLFAILWGLSGW
                     FQTFGAAPSVVALNQWFSHKERGTLYGIWFSAHNIGTGLTYVITAVIVVHYGWRMGLL
                     APGVLCLVASGFLYHFMFDRPQTYGLPPVAVYAGEQSTAAPAAEKALLWVQLAVLKRP
                     AVWILGLASAACYLTRYAFEMWGVLFLTNAKGYSLLGASTVVSISQFAGIAGAVTCGM
                     ISDRFFNHRRSAPCLIFGIIFVLSTAAFVYTPARHPWVDYLSMAFFGYSVYALVAYLG
                     GLMAVDITPRKATGAAMGVIGLFSYAGASIQEYLSGHLINTTRKIVDGVAVYDFTQAG
                     HFWVLGAVGACLLPLLVWNAKPQEDAP"
     gene            3930..5141
                     /locus_tag="PKI07_12815"
     CDS             3930..5141
                     /locus_tag="PKI07_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51965.1"
                     /translation="MKTLSSVDPVSRRSFLQTLGLGSAALAVAPAASGQEKKVIQGFE
                     EAPTAPDASRGWKPVSERKIRVGLVGYGVCRFGAVFGFQDHPNVEVVAVSDLIPDRCA
                     GLAKAARCARTYPSLEELVKDDRIEAVFVATDAPSHARHCLEVLKHGKHVASAVPAVF
                     GSVEEAHELFDAVKASGLKYMMFETSCFHEDLHGMRQVYTAGGLGKLVYSEGEYYHYM
                     DTPIDSYKGWRIGLPPQWYPTHSNAYYIGVTGGSFTEVSCMGMPSTIEHLRPANNVYK
                     NPFGTEIALFRTSDGGMARMAVSWDTPGLSGEMGRIRGQRGTYYGRYEGQEKKLPGLQ
                     RPPLPPGVAAGGHGGSHGYLTNEFITAILQDRRPLVDIAQALNMTVSGIVAHQSALKD
                     GELLKIPQFKL"
     gene            6136..>6777
                     /locus_tag="PKI07_12820"
     CDS             6136..>6777
                     /locus_tag="PKI07_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51966.1"
                     /translation="MKAKLTMQAMILSCGLAGLLLTGCAQHQSQARYEPAPAVTPAPA
                     PAPAPAAKPESFGPSHSTFEENGVRWVRGSMAFPTGLRESSGLLLEKTVPAQVLAGQK
                     FDYSYKVSNLTDHPIQMVIVNDRVSPNFSRAEAEPAATEVRDGVAVWQLGNLGPKESR
                     TIRIKGASAEEGAVSTCGWATYRPLLCEDIKVVKANIQLVKKAPAEVVICDPIP"
BASE COUNT         1296 a         2049 c         2245 g         1187 t
ORIGIN      
        1 ttactccgcc gctgggctgt ttcccgccct ttatcgagcg gcggagaggc tattccagga
       61 aggcgaaggc gccgtagccg acgacgtggt cgcgggggcc ggcggtgtcg ccggaggtgc
      121 gaaggtccag ggtgcgggca cgcaaatggc gttggcgggc gatttgcagc aggccgcgaa
      181 tggggatgcg gccgcaggcg cggtcgtcgc cgatgccctc gggattgagg gtctcaatgg
      241 cccgggcggt ggcgcggtcg agccggtgtg cggtctgctg atcctggtag tggctgaggt
      301 cggagctgat gacgatgcgg gtttcggggc cgccccagac ggtttccagc acttccgcaa
      361 cgtcctcggg cgcggcatcg ccgacggcta gcgggagaag ggtgaagccg cccaggacgg
      421 tttggaggaa gggcagttgg acttcgaggg agtgttcgcc ggcgtgggct tcgtcttgtt
      481 cccgcacttg cggcagggcg cggacccggc gggtggtttc gccgtcgagt ggcacctggc
      541 ccagcgggga ggcaaaggcc cgggcggagg aggtggcaag gccgtcaagc gcgacgcggt
      601 gcgaggggcc gaggagaata atgcgctgga tgagctggcg cgcggggatg aacccggcat
      661 aggccgaggc ggcgatgggg ccggagtaga tgtatccggc gtggggaacg atcagggcct
      721 tgggcgccgg gccatccgcc ggaggcgcct gggcgagcag ggtgcggacc aggccggcca
      781 gttcggcggg gtccgcggga tagaaacgtc ccgcaaaggc gggcggccgg attttgccgg
      841 tcgaggaggt gtctgctcga atcattcagt tccctttcag gccgcgcgga aaaatgcggt
      901 tgcgggggga ggaggccggg ccggccaacg ttttcccggt tttgtcagcc gaccagaggc
      961 cggggatggc cgtgccgcaa tcggggcagc cgccgccggg ggtgatccgg tattcgcgca
     1021 cccgaaagcc ccgccgctca atgagcgttg cgtggcaatg cgggcagcga gtgttttccc
     1081 acgggccaac ctggccgggg gcgttgccgg catagacgaa gcgcaggcct tcggcggcgc
     1141 cgatttcggc ggcccggatg agttgggcgg tggaagtggc ccgcgggccg gtcagtttgt
     1201 agtccgggtg aaacgcggtg acgtgccagg ggatgtcgcg gctgacagag gccaggaagc
     1261 gcgcgagctg gcgcagctcg gcttcctggt cgttgaagcc gggcacgatc agcgtcacca
     1321 cttcgagcca caatccgcgc tggtgaacca ggcggatgcc ttccgtgacc tgcccgagcg
     1381 tgccgccgag ggtgcggtag cgcttttcgt cgaagccttt caggtcaatc ttgcaggcgg
     1441 ccagccaggg gcgcaggaaa tccagcactt cgggggtggc gcagccattg gatacgaagg
     1501 cgccgtcgag gccggcggct ttgccttcct ggaagatggc ggcggcccat tcggcggtaa
     1561 tgagcggttc gttatagctg gacacgatca ggcgcgcgcc ggcgcgctcg ccggcgctga
     1621 ccagttgccg gggcgtgacc ggttgccagg gggcgcccgc cgcggcgtcg cgcaaggcct
     1681 ggctggtgac ccagttctgg cagaaggaac aatggaaatt gcaacccagc atcccgaagg
     1741 tcagcgcgcc actgccggga tagacgtggt agaagggctt tttctcgacc gggtcgcatt
     1801 gcacgcccgc gacgtagcca aaggggacgc gtaattgccc gtcccggcta aaacgaacgc
     1861 ggcatacgcc ggtccgcccg ggagcgatca ggcaacgatg tccgcaggca acgcaacgaa
     1921 tccgcccccc ctcgcttcgg gcaagcgccc caacggtcgt atgccgggcc agcacgtcgg
     1981 ccagcgtttc gcggggcggg cgggttgtgg ccggcgtgcc cggcgaagcg gagcccggcc
     2041 ccggcccggt ttgagcagtc acgtttggca taggccgcgc aggagcataa gccaatgttg
     2101 cctccgggcg gcgggttgtc aaagcgggaa ccggccgccg gaaccgaaag ccccgccgcc
     2161 ggcgttccct cccgcttccg ttcgcctcac ccggctaggg cgcatcctcc tggggcttgg
     2221 cgttccatac cagcagcggc agcaggcagg cgccgaccgc gccgagcacc cagaaatggc
     2281 cggcttgcgt gaagtcatac acggcgacgc cgtccacgat cttccgcgtg gtattaatca
     2341 agtggccgct gaggtattcc tgaatcgagg ccccggcata gctgaacagg ccgatgacgc
     2401 ccatggccgc gccggtcgcc ttgcgcgggg tgatatccac cgccatcaaa ccgcccaggt
     2461 aagccaccag cgcatatacc gagtagccaa aaaaggccat gctcaggtag tccacccagg
     2521 gatgccgggc cggggtatag acaaacgccg cggtggagag aacaaagata atcccaaata
     2581 tcaggcatgg cgcgcttcgc cggtgattaa agaaccggtc cgagatcatc ccgcaggtga
     2641 cggcgccggc aatccccgcg aattgggaaa tggagaccac cgtgctggcg cccagcagac
     2701 tgtagccttt ggcgttggtc aggaaaagca ccccccacat ttcgaaggca taccgcgtca
     2761 aataacaagc cgcgctggcc aggcccagaa tccagaccgc gggccgtttc aacacggcca
     2821 gttgcaccca caatagcgct ttctccgccg ccggggccgc cgtgctctgt tcccccgcgt
     2881 aaacggccac cggcggcagg ccgtaagtct gcggacggtc gaacatgaaa tggtaaagga
     2941 accccgacgc caccaggcaa agcacccccg gcgccagcag ccccatccgc cacccataat
     3001 gcaccacgat gaccgcggta atcacgtagg tcagcccggt gccgatgttg tgcgccgaga
     3061 accagatgcc atacaacgtg ccgcgttcct tgtgggaaaa ccattggttg agcgccacca
     3121 cgctgggcgc ggccccaaag gtttggaacc acccgctcaa gccccacagg atcgcaaaaa
     3181 ggtaaaaccc ggtcacgcat cccatcacca cgagcgtggc ggccgaaaga aacagcccgg
     3241 cggccaggaa gcgtttgatg ttcaaccggt cggccaaaaa cccgttgaag cacttgccga
     3301 tcatgtaggt gaaaaagaac acggacccaa tcgcccccat ttgctcgggg ttgaagacgc
     3361 cgccgtccag cagcggcttc ttcaccacgg cgaatccctg ccgcaccacg taatacaggg
     3421 catagcccag cgtgatggaa agaaataccg agatgcgccc ccggcggtag agccgcttga
     3481 tttcctcggc atctgccagc agcggctggt ctgctccggt cttgaagaag tcagccagcg
     3541 cgcccagtcc ccacggagcg cgccgtcctt tgccaggctg ccggtctgcc cgcgctgcgg
     3601 tcggaccggg tcgttctata ttgtccatgc gtgccattgt gcgccagagc cttcgggcgg
     3661 cgcggcgtca tctttcgctc cgcccgccgg atcgtcaaga cgatactcgc ctcgcgctat
     3721 gggccgggat ccaacggcgg aaagccccgc ggcggccgcg gcggggggcg cccggcactg
     3781 tggcggaggt cggagggcag gcgagggctg gcgaacgccg ttgatgcggc tggggaactg
     3841 ccgcgtaaag cttgacacgc aaaaagcgcc ggtcaagctg gagcaatatg cggcacaagc
     3901 atcttcccgg cgcaggaacc cctgcggtaa tgaaaactct atcctccgtt gacccggtct
     3961 cccggcgttc atttttgcaa acgctcgggc tcgggtccgc cgcgctggcg gttgcgcccg
     4021 ccgcttccgg ccaggaaaaa aaggtcattc agggcttcga ggaagcaccg acggccccgg
     4081 atgcttcgcg ggggtggaaa cccgtgtccg agcggaagat tcgtgttggg ctggtcggct
     4141 atggcgtttg ccggtttggg gccgtctttg gttttcagga tcacccgaac gtcgaggtgg
     4201 tggcggtcag tgatctgatt ccggaccggt gcgccgggct ggccaaagcc gcccgctgcg
     4261 cccggacgta tccctcgctc gaggaactgg tcaaggatga ccgcatcgag gcggtgttcg
     4321 tcgccaccga cgcgcccagc cacgcgcggc attgcctcga ggtgctcaaa cacggcaaac
     4381 acgttgcctc cgccgttccg gcggtgttcg gctcggttga ggaggcccac gaattgttcg
     4441 acgccgtcaa ggccagcggg cttaaataca tgatgttcga aacctcctgt ttccatgagg
     4501 acctccatgg catgcggcag gtttataccg ccggcgggtt gggcaaactg gtctattccg
     4561 agggggaata ctaccattac atggatacgc cgatagactc ctataaaggc tggcgcatcg
     4621 gcctgccccc ccaatggtat ccgacgcact ccaacgccta ctatatcggc gtgaccgggg
     4681 gcagctttac cgaagtaagc tgcatgggca tgccgagcac gatcgagcat ctgcgcccgg
     4741 cgaacaatgt ttacaaaaac cccttcggca cggaaatcgc cctgttccgc accagcgacg
     4801 gagggatggc gcgtatggcg gtcagttggg atacccccgg gttaagcggt gagatgggcc
     4861 gcattcgggg acagcggggg acttattatg gccgttatga ggggcaggag aagaagttgc
     4921 ccgggctcca gcgcccgcca ctgcctcccg gggtggccgc cggcggccac ggaggttctc
     4981 atggctacct gaccaacgag tttatcacgg ccatcctcca ggatcgccgc ccgctggtgg
     5041 acatcgccca ggctttgaac atgaccgtca gcggtatcgt tgcccatcag tcagccctga
     5101 aagacggcga gctgttgaaa ataccccaat tcaagctcta agccggggcg attccggtct
     5161 tacccccaat tcgacctcac aggacgccca agtccttgct tttaagggcg aaaggcattc
     5221 ggaagccagc ggccaagccg ttgaatccct aacccaacag atcgtcgagg ctgactgccg
     5281 gcccgtcagg gccacttcgg caccagtgga gcgaaggcat acagttagac tacgcaacgt
     5341 caacgtttca gataaggcat tcaaaagcca tccggacgcc cgctcgccat tgaaaagccg
     5401 cttttcgcca tccctggctt tataactgac tgcttttgca gaacttagta attatcttcc
     5461 cgtcttttct cttcgttccg gcctttccga acgcccagac ccacgatcca gataccgtga
     5521 aaataccctg cccccaggcc gtgtacaagg ggtgtacaag ggatgctcac cgggagtcca
     5581 taggatttta gctggtgcat ccgatgggca tcccttgtac accccttgta cacggcctga
     5641 aggcagggta aatccaaggg gcgtccaagg tacgccgaag ccggtaaaac ccaggttttg
     5701 cccctgtttt gcctctcttt atgccgaaac tcttgttttt accggcgaaa ggctcgggat
     5761 tcagcagcta tgctcttgaa tttgctaaat aaagggttcg tcggggctat ggtctgggcg
     5821 gtcaggcccg cccaggctcc gatggaacgc gggagggcgg ttgaactgcc caactgaatc
     5881 gttccggcta aatcggggca cggagtggca tgggcggtcg cagaaaaggc gggtaaagca
     5941 taagttagcg cggagggaca ggcctgttcg cgcttaacag ggcatcgccg ttttccagca
     6001 atttttcttg cgtccagtct aaattggcct tagattctgc ggcaggtaat tggatggatt
     6061 ccaaagtgtt ccgtcattat cttttgcaat cggacggaga ataacaggat tgaatagaag
     6121 taaattggac gacgtatgaa agccaaactg accatgcagg caatgattct aagctgcggc
     6181 cttgccggcc tgctgctgac cgggtgcgcg caacatcaat cgcaggcccg gtatgaaccc
     6241 gctcccgccg tcaccccggc tccggccccg gctccggctc cggcggccaa accggagagc
     6301 ttcggcccct cgcattcgac ttttgaggag aatggcgtcc gctgggtccg gggttcaatg
     6361 gcgtttccca cggggctgcg tgaaagcagc gggctgttgc tggaaaagac cgttccagcg
     6421 caagtgctgg ccgggcagaa gtttgattat tcttacaagg tcagcaacct gacggatcat
     6481 ccgattcaga tggttatcgt gaatgaccgg gtatccccga atttctcccg ggctgaggct
     6541 gagccggcgg ccacggaagt gcgggatggc gtagccgtct ggcaactggg gaacctgggc
     6601 cccaaggaaa gccgaacgat tcgcatcaag ggggccagcg ccgaggaggg agcggtatcc
     6661 acttgcggct gggcgaccta tcgcccgttg ctgtgcgagg atatcaaggt ggtaaaagcc
     6721 aacattcagt tggtgaagaa ggcgccggcg gaagtggtga tttgcgatcc gattccg
//
LOCUS       DAOPQG010000149         6744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_12468_length_6744_cov_16.218867, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000149 DAOPQG010000000
VERSION     DAOPQG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12468_length_6744_cov_16.218867"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            813..1799
                     /locus_tag="PKI07_12825"
     CDS             813..1799
                     /locus_tag="PKI07_12825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC51967.1"
                     /translation="MNETLPSVTVVIAARPRQADVKAVAASRALDYPADRLAILVARG
                     AQPSVQRNAAVKAASGELVYFLDDDSAPAPGNLRRAVEHFRDPRVQMVGGPNLCPPEA
                     PPLEQVFARVLASELAFGPSRARYAAVGRTRATSEKELILCNLLARRSALLELGGFDE
                     TLYPNEENALMDQLQRRGGKLLYDPQLVVWRRPRASLRAFARMLMTYGRGRAEQFRRH
                     PTPGSALNFVPPLFCLYLLALLPLWWLTPLGKLSLWPLAGYALAVLAQGLVSAVRGPV
                     WQSVAALPLVVLTHLLYGAGFWRGLFTRLTPPAPSAPSQVVLETPPPRNAGG"
     gene            complement(1819..2637)
                     /locus_tag="PKI07_12830"
     CDS             complement(1819..2637)
                     /locus_tag="PKI07_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOC51968.1"
                     /translation="MIAQPQTIKSLDRPELQPYRTMRRQAEHRQQGIFVAEGEKVVRR
                     LLESQFPVISVLLPEKWLRELEPLLRTRRELIQVFVAEKKLLETLTGFSMYQGLLAVG
                     KVPPQPTLADILARSPPPRLLAAADGLSSAENLGALVRNCAAFAAQALLVGETCANPF
                     LRRAVRSSMGAIFHLPIVETSNLARDLRALRQQGIRCLAAHPHTDGRILSQASLAGDC
                     CLVFGSEGHGLSPAALAACDDAAAIPMPPTVDSLNVGSAAAIFLYEANRQRGRM"
     gene            complement(2652..3434)
                     /locus_tag="PKI07_12835"
     CDS             complement(2652..3434)
                     /locus_tag="PKI07_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC51969.1"
                     /translation="MPVHLTILGSGSSGNCAYVETAETRLLIDAGLSLRQIRQRLASI
                     GRAPEKLTGILITHEHSDHVQSLPALSQKLHIPVYCNRPTLDAIEYQFQTRLPGHLFA
                     TGASFEVGDIHVETFSVPHDAQDPVGFLLRTSAGNLGFLTDLGHATRLVLERVRPAHA
                     LVLEANHDVQMLQNCPRRPWSLKQRILGRYGHLSNEAAADALEQIISPDLRHLYLGHL
                     SRECNLPELAYRIVHERLQKLGARHVRLELTSQAQPCPTLSL"
     gene            complement(3516..4361)
                     /locus_tag="PKI07_12840"
     CDS             complement(3516..4361)
                     /locus_tag="PKI07_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC51970.1"
                     /translation="MNRQPARPGGFTLIELLVVIAIIGLLAALLLPALAKSKLKAQAI
                     QCMNNHRQLCLAWRMYSDDNQDRLLYASEDYANPATLAETWVTGTLNFSPWNPSNWDP
                     NQDITQSPLWPYCGRNLGIWKCPSDRSFVEVGGECKPRVRTMSMNIFLGGWGGTDGNW
                     GPAFSDYLIYLKQGELQVPGPAKIFVFLDMRPDSIDMGNFGTRMAGYPNTPSRYGFFD
                     LPGFYHHLAGGFSFADGHSELRRWRDPRTTPPLVEGGLVPDMFDSPNNPDVAWLQDRA
                     SRPKN"
     gene            complement(4443..5153)
                     /locus_tag="PKI07_12845"
     CDS             complement(4443..5153)
                     /locus_tag="PKI07_12845"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOC51971.1"
                     /translation="MITIRSFVVLGLSLILGLTIFGFQIGRAVRTGREFDRCLTVKGL
                     SEREVKATLAIWPIQFSTVAEELATLKSAMESNRVVVTSFLQARGISPDEIVHGLPVV
                     NDRQDERIHANRPALVRYRGVTTLVVRSANVDVVKKAIQDADALLDQGITLVGNEFAD
                     RTEFIFNAVNEIKPDMIREATANARAAAEKFAQDSHSKVGRIRRATQGALEIENRDAA
                     SPEKKVLRVVTTVDFFLD"
     gene            5594..6298
                     /gene="bioD"
                     /locus_tag="PKI07_12850"
     CDS             5594..6298
                     /gene="bioD"
                     /locus_tag="PKI07_12850"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOC51972.1"
                     /translation="MARIIFVTGSDTGVGKTLLTGLLLHHLRRRGCHALAVKPFCSGS
                     RADAEFLGALQDGELAADEIAPFFFSAPLAPLAAARRRHIIRLAEVRRHLKRMACRCE
                     CLLIEGIGGLLVPLGKGFTVRDLIVAIGSEVIVVSRNRLGTLNHTLLTGAALQQVGIK
                     DFTTVLMTVPPGDASSVTNRLILAEMLAPNPVFELGFLGRNPVRPGAVKLAAKKVEKT
                     LARILGCARMGASGSL"
BASE COUNT         1062 a         1925 c         2450 g         1307 t
ORIGIN      
        1 cacctgggga tgcgctaatt gcctgacgga gcgcgggcat attgcccgct tatcgagtag
       61 gcaacccgca aagcgggcag gatgcccgcg cgccgccggc agtttcgtgc atgctgcggg
      121 cgccttgccc ttgccgcgcg tccgactgag ctgcgcctcc acctggggat gcgctaattg
      181 cctgacggag cgcgggcata ttgcccgctt atcggatggg caacccgcaa agcgggcagg
      241 atgcccgcgc gccgccggca gtttcgcgcg tacgcgcggg cgcctgccct tgccgcgcgt
      301 ccaactgagc tacgcctcca cctgggatgc gctaattgcc tgacggagcg cgggcatatt
      361 gcccgcttat cggctgggca acccgcaaag cgggcaggat gcccgcgcgc cgccggagtt
      421 tcgcgcgtgc tgcgggcgcc ttgcccttgc tgcgcgtctg actgagcttc gcctccaccc
      481 ggggatgtgc taattgcctg acggagcgcg ggcatattgc ccgcttatcg gatgggcaac
      541 ccgcaaagcg ggcaggatgc ccgcgcgccg ccggcagttt cgcgcgtacg cgcgggcgcc
      601 tgcccttgcc gcgcgtccaa ctgagctacg cctccacctg ggatgcgcta attgcctgac
      661 ggagcgcggg catattgccc gcttatcggc tgggcaaccc gcaaagcggg caggatgccc
      721 gcgcgccgcc ggagtttcgc gcatgctcgg gcaccttacc cgtgcgcact ttccgctttg
      781 cactggttgc tgcgggactt tagcctgccg gggtgaacga gaccttgccg agcgtcacgg
      841 tggtgatcgc ggcccgaccc cgccaggccg acgtcaaagc cgtggccgcc agccgcgcgc
      901 tggattatcc ggccgaccgg ctggcgatcc tggtggcgcg cggcgcgcag ccttccgtgc
      961 agcgcaacgc ggcggtgaag gccgcgtcgg gggagctggt ttacttcctg gacgatgatt
     1021 cagcgcccgc gccgggcaac ctccgccgcg ccgtggagca ttttcgcgac ccgcgggtgc
     1081 agatggtcgg cgggcccaac ctctgcccgc ccgaggcgcc gccgctggaa caggtcttcg
     1141 cgcgggtgct ggccagcgag ctggcttttg gccccagccg cgcccgctat gccgcggttg
     1201 gccggacgcg ggcgaccagc gagaaggagc tgatcctctg caacctgctc gcccggcgaa
     1261 gcgccctgct ggagctgggc ggctttgacg agacgctcta tcccaacgag gaaaacgcgc
     1321 tgatggacca gttgcagcgc cgcggcggaa aattgctcta cgacccccaa ctggtcgtgt
     1381 ggcgccgccc gcgcgccagc ctccgcgcgt tcgcccggat gttgatgacc tatgggcggg
     1441 ggcgggcgga acagttccgc cgtcatccca cccccggctc ggcgctcaac tttgtccctc
     1501 cgctcttttg tctttacctg cttgcgctcc tgccgctgtg gtggctgacg ccgcttggga
     1561 agctcagcct gtggccgctg gcgggctatg ccctggccgt gctggcgcag ggcctggtgt
     1621 ccgccgttcg cgggccggtc tggcaaagtg ttgccgccct tccgttggtg gtgctgacgc
     1681 atctgcttta cggcgcgggc ttttggcgcg ggctttttac gcggctgacg cccccggcgc
     1741 catccgcccc gagccaggtc gtgctggaga cgccgccccc gaggaacgcg ggcggttgaa
     1801 ctgcccgggg gggcgcggtt acatccggcc gcgctggcgg ttggcttcgt agaggaagat
     1861 ggcggcggcg ctgccgacgt tcagggagtc cacggtgggc ggcatcggga tggcggcggc
     1921 gtcgtcgcag gccgccagcg ccgcgggaga gaggccgtgg ccttcgctgc cgaagacgag
     1981 gcagcagtca ccggccaggc tggcctggga gaggatgcgg ccatcggtgt ggggatgggc
     2041 ggcgaggcag cggatgcctt gctggcgcaa agcgcggagg tcccgggcca ggttggaggt
     2101 ttccacgatg ggcaggtgga agatggcgcc cattgagctg cgcacggcgc gccgcaggaa
     2161 ggggttggcg caggtttcgc ccacgagcag ggcctgggcg gcgaaggcgg cgcaattgcg
     2221 gacgagggcg cccaggtttt cggcgctcga gaggccgtcg gcggccgcca gcaagcgcgg
     2281 cggggggctg cgggcgagga tgtccgccag cgttggctgg ggaggcactt tcccgaccgc
     2341 cagcaggccc tggtacatgg agaagccggt gagcgtttcc agcagctttt tttccgcgac
     2401 gaagacctgg atgagttcgc gccgggtgcg caggaggggt tccagttccc gcagccactt
     2461 ttcggggagc agcacagaga tgacggggaa ttggctttcc agcagccgcc gcaccacttt
     2521 ttcgccctcc gccacgaaga tgccttgctg ccgatgttcg gcttggcggc gcatggtgcg
     2581 gtagggctgg agttcgggcc ggtcgagcga tttgatcgtt tggggttggg cgatcacggc
     2641 cgcgcgggac tttacaggct cagggtgggg cagggctggg cctggctggt cagttcgagg
     2701 cgcacgtgcc gggcgccgag tttttggagg cgctcgtgga cgatgcggta ggccagttcg
     2761 gggaggttgc actcgcggct caggtggccg aggtacaggt ggcgcagatc gggggagatg
     2821 atttgttcga gggcatcggc ggcggcttcg ttggagaggt ggccgtaccg tcccaggatg
     2881 cgttgtttga ggctccacgg ccggcggggg cagttttgca gcatctggac gtcgtggttg
     2941 gcttccaata ccagggcgtg ggcggggcgg acccgttcga gcaccaggcg ggtggcgtgg
     3001 cccaggtccg tcaggaaccc gaggttgccg gcgctggtgc gcaggagaaa gccgaccggg
     3061 tcttgcgcgt cgtgcggcac gctgaaggtt tcgacgtgga tgtcccccac ctcgaagctg
     3121 gcgccggtgg caaagagatg gccgggcagg cgggtttgaa actggtattc gatggcgtcc
     3181 agcgtggggc ggttgcagta aacgggaatg tggagtttct ggctcaaggc cggcaggctt
     3241 tggacgtggt cggagtgttc gtgggtgatg agaatccccg tgagcttttc cggcgcccgg
     3301 ccgatggagg cgagtcgctg gcggatctgg cgcaggctga gtccggcgtc aatgagcagg
     3361 cgggtttctg cagtttccac gtaggcgcaa ttgccgctgg agccgctgcc caggatggtt
     3421 agatgaacag gcacaattta ccttacggag gggccggggg cggggcaagc tcttcggggc
     3481 gggggttggg ggcggagcgc gggccggcgc ggcgcttagt ttttcgggcg tgaggcccga
     3541 tcctgcagcc aggcgacgtc ggggttgttg ggggagtcga acatatccgg caccaggccg
     3601 ccttccacga gcgggggcgt ggtgcggggg tcgcgccagc ggcgtagttc ggagtggccg
     3661 tcggcaaagg agaagccgcc ggccaggtga tgatagaagc ccggcaggtc aaagaagccg
     3721 tagcgggagg gggtgttggg ataaccggcc atgcgggtgc caaaatttcc catatcaatg
     3781 ctgtccgggc gcatatcgag gaagacgaag attttggcgg ggccgggcac ctgcaattct
     3841 ccttgtttca ggtaaatgag gtagtcgctg aaagcggggc cccagttgcc atccgttccg
     3901 ccccagccgc cgaggaagat gttcatggac atcgtgcgca cgcggggttt gcactcgccg
     3961 ccgacttcga caaaggagcg gtcggaaggg catttccaga tgcccaggtt gcggccgcaa
     4021 tagggccaga gggggctttg ggtgatgtcc tggttcgggt cccagttcga ggggttccag
     4081 gggctgaaat tgagtgtgcc ggtcacccag gtttccgcca aggtggcggg gttggcgtag
     4141 tcttcgctgg cgtagagcag gcggtcctgg ttgtcatctg aatacatgcg ccaggccagg
     4201 cacaattggc ggtggttgtt catgcactgg atggcctggg ctttgagttt gcttttggcg
     4261 agcgccggca gcaggagcgc ggccaggagg ccaataatgg caatcaccac cagcagctca
     4321 atcagcgtga aaccgccggg gcgcgcgggc tggcggttca cgcgcgcgcg tcgaatttgt
     4381 tcagtcgcat gcaatatcct gtccgtgggc ggccccggcg cacgggcgcg gggccggcgg
     4441 tttcaatcca ggaagaaatc aacggtcgtc acgacgcgga gaaccttctt ttcgggcgag
     4501 gccgcatccc ggttttcgat ttccagggcg ccctgggttg cccggcggat gcggccgact
     4561 ttgctgtggg agtcctgggc gaacttttcc gccgccgccc gcgcgttggc ggtggcctcc
     4621 cggatcatgt ccggcttgat ctcattcacc gcgttgaaga taaactcggt tcgatccgca
     4681 aactcattgc ccaccagggt gatgccttga tccagcaacg catcggcatc ctgaatggct
     4741 ttcttgacta cgtccacgtt ggcgctccgc acgaccagag tggttacgcc gcgatagcgc
     4801 accagggcgg ggcggttggc gtgaatgcgc tcgtcctggc gatcgttgac gaccggcagg
     4861 ccgtgcacga tttcgtcggg ggatatgccg cgggcttgta aaaacgaggt gacgacgacg
     4921 cggttggatt ccatcgccga cttgagggtc gccagctcct cggcgacggt ggaaaactgg
     4981 attggccaga ttgccagcgt ggctttcact tcccgctctg acaatccttt gacggtcagg
     5041 cagcggtcga actcgcgccc cgtccgcacg gcgcggccaa tttgaaagcc gaagatggta
     5101 aggcccagga tcagtgacag gccgaggacg acgaatgaac gaatcgtaat catggttttg
     5161 ctttcgacat taacgaacgt attttgagtt ttgccaacta cccgccgggc ggcgtttgtc
     5221 tcaccagcgc ctcctcccag agggaatcgt cacattccgg aagattttcg cgcattccgg
     5281 ctgccgggtc aagcctgcgg gagttcgttg cgcggccttc gggctgccgg aaacggaggc
     5341 ggttggcggc tggcttcggg tggcccggct tcttcggcga gaagacgcga ggctgggcag
     5401 ttgcggttgc ggatttgtcc tggggtctgc gcagggcggg ggactttcag ccgcgttgtt
     5461 tctagatcgg ctcaagctgg ggcagtggca ggttgaccct gtattggctg gaagcctggc
     5521 agcggccggg agcgctttag gtctgtccgt ttggcggcgg ctgtgctaat gctccgccgt
     5581 gaacagatct ggcttggcgc gcatcatttt tgtcacgggc agcgataccg gcgtggggaa
     5641 gacgttgttg accgggctgc tcctgcacca tttgcggcgg cggggctgcc acgccctggc
     5701 ggtcaaaccg ttctgctccg gcagccgggc ggatgcggaa tttttgggcg ccctgcagga
     5761 cggcgaactc gcggcggacg aaatcgcgcc tttctttttt tccgcgccgc tggctccgct
     5821 ggcggcggcg cgccgacgcc atatcatccg cttggccgag gtccggcggc atctcaagcg
     5881 catggcgtgt cgctgcgaat gtctgctcat cgaggggatt ggcggtttgc tcgtgccgtt
     5941 ggggaagggg tttacggtgc gagatttgat tgtggcaatc gggagcgagg tcatcgtggt
     6001 ttcccgcaat cgcctgggca cccttaatca cacgctgctc accggggcgg cgttgcagca
     6061 ggttggaatt aaagatttta cgacggtgct gatgacggtc ccgccggggg atgcatcgag
     6121 cgttaccaac cggctgatcc tggcggaaat gctcgcgccc aacccggttt ttgagcttgg
     6181 atttttgggg cggaatccgg tgcggccggg ggctgtaaaa ttggcagcaa aaaaagttga
     6241 gaaaacactt gcgcggattt tgggctgtgc taggatgggt gcttctggtt ctttgtgagt
     6301 ctggttttcg gacggcgaaa aaaagttgaa aaaagatttt gactgcctcc ggaatgcgtg
     6361 tagattgttg gcagttgcga gtaagcagct ccggtaaaaa aagctgaaaa aagtttttga
     6421 catccggcgc ggctctgata aagtggccgc gaaattaaca aaggtccgag aggccgagtt
     6481 aagagtgatt aagtcggcgc tcgggatttt tgtccctcgg taacgagagg gaagcgggca
     6541 caaaatggtc ccgcggacgg gttgagccgg gatcggcgcg ggaaaagagc acgagctggc
     6601 gagcttgtcc gggaaaagaa ttttacgggt ggtgacaccc gctgctcatt gaaaattgaa
     6661 ttgtgcgata agttaagagc cccttcaggc gggtcttgtt agtagggatc cgtttggaga
     6721 gttttaaacc aaaagaccgt ccgg
//
LOCUS       DAOPQG010000150         6652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_12679_length_6652_cov_14.461119, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000150 DAOPQG010000000
VERSION     DAOPQG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12679_length_6652_cov_14.461119"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1888
                     /locus_tag="PKI07_12855"
     CDS             74..1888
                     /locus_tag="PKI07_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC51973.1"
                     /translation="MSTKRTVSQSPPRPRPTAQRKKSAASPALPPLVRDIFARTRTAA
                     FRRYLRELLLELCRIDTTPNPKVAQMQAAEAACFRILERELGGLAFADARLERRPINP
                     GIQAHPNYSLLHFTKTPRRPQGLSPEATYANRSNLLYFVPGGGGGGSEGQSVALNAHI
                     DVVAPYFPPRVQGDTVYGRGACDDKGPLVGIVAALKVLSEVLARAGRRLNRNVLAMLV
                     VEEETGGNGSLSLAVDRELRKFYDSILVAECTDLKIHPANRGAVWYRAALNAPPGVSA
                     FEMFAFVNEELEKEGAALRAESRHPLFPQRPVQTCHGMIGPFGEHPSRICGEVSFAIR
                     FDRAPDRPTELLVEDCLEAGLAGYLGLYGDKTRVTDPATGKPMVARHYDWRRESDGFR
                     VDVHGATGHMGAIRERDGAITKMAHLVRSLVFSRAKLEARAQGRVAFELARPAHGEGL
                     PPGAALVLEGGQGFIPTHGITEVMERLRQAAQRGAGNYLRRIGRFERGEDVVTVAYEK
                     LHNVAFDGDPDSRSVRHAVAAAKLGGFWRDEPILGWTVSCDARLFATEYPELEVLTFG
                     PGQLAFAHSDEEQIRLEDLRAAVEFLAIFILRQTGTLA"
     gene            1894..3084
                     /locus_tag="PKI07_12860"
     CDS             1894..3084
                     /locus_tag="PKI07_12860"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51974.1"
                     /translation="MKTQLRLGVIGSGSVVREIYQYLYFRSQYSPLLDVCAVADPSEP
                     YRNWFGNLAGIPADRRFADHRELLAKVELDAVQVNTPDHLHCAPTVDALRAGLDVVVP
                     KPTAATVRDAHAMIAAARESGRFLGIDFHKREDPRIKEAEARFQRGRYGTFQTAVFYM
                     LDKLWVADPNHSPRFFASADFAEKNTPVSFLTVHMADALMKIVNLIPVQVRATGYSQK
                     LPALKPIPVNGYDLVDTEIRFTTGAQAHIITGWAVPNSAESVTVQSGRILCSEGLMDL
                     GLGTPGLREVHADGLTEVNPLFRNFEKDNTVTGYGISSPGRLYQQILALRNGRCSDAE
                     RAAAFHPMTLGFYTTLVLEGAEKSLATGAKSPEGVTHGPVVDLRRLAAEQLGASVAAA
                     YGLQ"
     gene            complement(3147..3401)
                     /locus_tag="PKI07_12865"
     CDS             complement(3147..3401)
                     /locus_tag="PKI07_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51975.1"
                     /translation="MRRIKIWVLAGCMGGLLAGSALAAEAAPRKLTCCEKAAAEGREC
                     RNKCCIIAHKQGKSCEKCNPHKEDLKFLKNAPPADNAAAK"
     gene            3605..4147
                     /gene="nuoB"
                     /locus_tag="PKI07_12870"
     CDS             3605..4147
                     /gene="nuoB"
                     /locus_tag="PKI07_12870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOC51976.1"
                     /translation="MQRSTELGYNSKVEGDVIVTRRSAALNWLRRGLEGGMNWGRKNS
                     LWPMPMGLACCAIELMGTGASRYDISRFGAEVMRFSPRQSDVMIVSGTVTYKMARVVQ
                     RLFEQMPEPKWVIAMGACASTGGMYRSYAVLQGIDRLLPVDVYVGGCPPRPEALLDAL
                     MKLQDKVAGEPSLATLKRVA"
     gene            4144..4737
                     /locus_tag="PKI07_12875"
     CDS             4144..4737
                     /locus_tag="PKI07_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOC51977.1"
                     /translation="MTARELALQLGAQFPGLVSAPAEFRGEITLRVAEAERMPEVCAA
                     AKAELGFDYLVDMTSVDNYGQDPRFTVVYHLYSYRHHCWLRLKTAVSEEQSELPTVTG
                     VWRTADWHEREIYDMMGLRFRGHPDLRRILMWEGYPYFPLRKDFPLAGRPSDVPEVAF
                     TGAAPLEGGPFVTTAGGADAAAREPRARTPESEPPRD"
     gene            4740..6017
                     /gene="nuoD"
                     /locus_tag="PKI07_12880"
     CDS             4740..6017
                     /gene="nuoD"
                     /locus_tag="PKI07_12880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOC51978.1"
                     /translation="MAEAHELKFKDTAARAAAAAACSAPPPAEAEPGDLAGDRMVLNV
                     GPSHPATHGVLRLVIELDGERITRVRPEIGYLHRGDEKIAENCTYTQFIPYTDRLDYL
                     APMANNVAYALAVEKLLGLERQLPPRCQYIRVICAELARLSSHLLGIGCYAMDCGAMT
                     VFLHTFTEREKIYNLCESLTGARFTTTYTRIGGLARDLPPGWVEQCRQFLDEVVVNIE
                     ETETFLTRNRIFMDRTQGVGVIPREVAIDYGLTGPNLRASGVAHDLRKAHPYLVYDQL
                     QFEVPVGTRGDCYDRYRVRMEEMRQSVRIVRQCLDQLPDGPVNAPAAKVLLPPKARVL
                     TKMEELIHQFMNVTQGVNAPPGEVYFAHENPKGELGFYLDSRGGGTPYRLKIRSPSFV
                     NLSILSWLLPGHLLGDIVAVLGSFDFVMGECDR"
     gene            6014..6550
                     /locus_tag="PKI07_12885"
     CDS             6014..6550
                     /locus_tag="PKI07_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOC51979.1"
                     /translation="MSEIRPLEPIPSPLREQPDFAVPPALAAELDAVIARYPQARSAS
                     LMLLHAIQEQFGYISRQAVEWVAARLGLPPLNIYELVTFYPMFHAAPVGKYHLKICRT
                     LSCALGGSHQLHELCCTRLGLDPRQHGPQTTKDGKFTVEFVECLAGCGAAPVMMVNDA
                     FHEGVTPRRAEEILGGCS"
BASE COUNT         1064 a         2089 c         2346 g         1153 t
ORIGIN      
        1 ttatttgtgg cggggaaagc tttgccttga cagccggggt gcttttgcta gcctgtcagg
       61 gcttcgagaa accatgtcaa caaaacgcac cgtgagccaa agccctccgc gcccgcgccc
      121 aaccgcccaa cggaagaagt ccgcggccag cccggctttg ccgcccctgg tgcgcgatat
      181 ctttgcccgc acccgcacgg ccgctttccg gcggtatttg cgggaactgc tgctcgagtt
      241 gtgccggatt gataccactc ccaatcccaa ggttgcgcag atgcaggcgg cggaggcggc
      301 ttgttttcgc attctcgagc gcgagctggg cggcctggct tttgccgatg cgcgcctcga
      361 acgccggccg atcaatccgg gcattcaagc gcatccgaac tattcgctgc tgcatttcac
      421 caagaccccc cggcggccgc agggcttgtc gccggaagcg acgtatgcca accggagcaa
      481 cctgctttat tttgttcccg gcggcggcgg gggcggcagc gagggccagt ccgtggccct
      541 caacgcccat atagatgtcg tggcgcctta ttttcccccg cgggtccagg gcgacacggt
      601 ttatggccgc ggcgcgtgcg atgacaaggg cccgctggtg ggcatcgtcg ccgcgctcaa
      661 agtgctttcc gaagtcctgg cccgcgccgg gcggcggctg aaccgcaacg tgctggcgat
      721 gctggtggtc gaggaggaaa cgggcggcaa cgggtcgctc tcgctcgccg tggatcggga
      781 gttgcggaag ttttacgaca gcattctcgt ggccgagtgc accgatttga agattcaccc
      841 ggccaaccgc ggcgcggtgt ggtatcgggc cgctttgaac gcgccgccgg gggtttcggc
      901 gttcgagatg tttgcgttcg tcaacgagga gctggagaaa gaaggcgccg ccctccgcgc
      961 cgagagccgc cacccgctct tcccgcagcg gccggtgcag acgtgccacg gcatgatcgg
     1021 cccttttggc gagcacccga gccggatttg cggcgaggtc agcttcgcca tccgctttga
     1081 ccgcgcgccg gatcgcccga cggaattgct ggtggaggac tgtctggagg ccgggttggc
     1141 gggctacctg ggcctgtatg gggacaagac ccgggtgacg gatccggcga ccggcaagcc
     1201 gatggtggcg cgccattacg attggcggcg ggagagcgat ggtttccggg tggacgtgca
     1261 tggcgcgacc ggccacatgg gcgccattcg cgagcgcgac ggggccatca cgaagatggc
     1321 ccacctggtt cggagcctcg tgttttcgcg ggcgaagctg gaggcccgcg cccaggggcg
     1381 cgtggctttc gagctcgccc gcccggcgca cggcgagggg ctgccgcccg gcgcggcgct
     1441 cgtgctggag gggggccagg ggtttattcc cacgcacggc atcacggaag tcatggaacg
     1501 gctccgccag gcggcccagc gcggggcggg caattacctc cgccgcatcg ggcgattcga
     1561 gcgcggcgag gacgtggtca cggtcgccta cgagaagctg cataacgtgg cgttcgatgg
     1621 cgacccggat tcccggtcgg ttcgccacgc ggtggcggcg gcgaagctgg ggggcttttg
     1681 gcgggacgag cccatcctgg gctggacggt ttcctgcgat gcgcgcctgt tcgccactga
     1741 atacccggag ctggaggtgc tgacctttgg cccggggcaa ctggctttcg cccactccga
     1801 cgaggagcaa atccggctgg aggacctccg cgccgccgtc gagttcctgg cgatctttat
     1861 tctgcggcag acgggaacgc tcgcgtaatc actatgaaaa cccaactcag actcggtgtc
     1921 atcggctccg gctcggtcgt ccgtgagatt tatcaatacc tgtatttccg cagccagtat
     1981 tccccgctgc tggacgtttg cgccgtggcc gatcccagcg agccgtatcg caactggttt
     2041 ggcaacctgg ccggcattcc cgcggaccgg cgctttgccg accatcgcga gctgctggcc
     2101 aaggtcgagc tggacgccgt tcaggtcaac acccccgacc acctgcactg cgcgccgacg
     2161 gtggacgcgc tgcgcgccgg gctggatgtg gtggtgccca agccgacggc cgccacggtg
     2221 cgggacgccc acgcgatgat cgcggcggcg cgggagtcgg ggcggttcct gggcattgat
     2281 ttccacaagc gcgaagaccc gcgcatcaag gaagccgagg cgcggttcca gcggggccgc
     2341 tacggcacgt tccaaaccgc ggtcttctac atgctggaca agctgtgggt ggcggacccc
     2401 aatcattccc cgcggttttt tgcctcggcg gactttgccg agaagaacac gccggtatcg
     2461 ttcctcacgg tgcacatggc cgacgcgctg atgaagatcg tcaacctcat ccccgtccag
     2521 gtccgggcca ccggctattc ccagaagctg cccgcgctca agcccatccc ggtcaacggc
     2581 tacgacctgg tggatacgga gatccggttc acgaccgggg cccaggcgca tatcatcacc
     2641 ggctgggccg tgcccaattc cgcggaatcc gtcacggttc aatccgggcg catcctctgc
     2701 tccgaggggc tgatggacct ggggctgggc acgcccgggc tgcgggaagt tcacgcggac
     2761 gggctgacgg aggtcaatcc cctgttccgt aatttcgaga aagacaacac cgtgacgggc
     2821 tacggcatca gcagccccgg gcggttgtac cagcaaatcc tggcgctgcg caatggccgg
     2881 tgctccgacg ccgagcgtgc ggcggcgttt cacccgatga ccctcggttt ttacaccacc
     2941 ctggtcctcg agggggccga aaaaagcctg gccaccggcg cgaaatcgcc ggagggggtc
     3001 acgcatggcc cggtggtgga tctgcgccgg ctggccgccg agcagcttgg ggcttccgta
     3061 gccgcggcct acgggcttca gtagccggcg gggctgcggc cgcccgcggg gcgttgtccg
     3121 gggcgcccgg gagcgggggg cggggcttac ttcgcggcgg cgttatcggc cggcggggcg
     3181 tttttgagga acttcaggtc ttccttgtgg gggttgcact tttcgcagga tttgccctgt
     3241 ttatgggcga tgatgcagca tttattccgg cactcccggc cttcggcggc ggccttttcg
     3301 cagcacgtca gcttgcgggg ggccgcctcc gcggccaggg cggagccggc cagcaacccg
     3361 cccatacaac ccgccagaac ccagatttta atccgtctca taaaagtcaa tacgctgatg
     3421 gttccatcgc cgcccgcgcg gcccgcggcc cggggcgcgg ccaccggagc atttcatcat
     3481 cagacgggga ttgtcgaggg ggaattcacg ggccggcgcg gaaaagtcac agattcaact
     3541 tgctggcgcg ggggcggttg gtaggctatg gggcgtgcgg cagtcccgac cgccgcgcgg
     3601 acctatgcaa cgaagcaccg aattgggcta caactcgaag gtagagggcg acgtcatcgt
     3661 cacccgccgt tcggcggcgt tgaactggct gcgccggggg ctggaggggg ggatgaactg
     3721 ggggcgcaag aattcgttgt ggccgatgcc gatggggctg gcgtgctgcg ccatcgagct
     3781 gatgggcacc ggggccagcc gctacgacat ctcgcgcttt ggggccgagg tgatgcggtt
     3841 ctcgccccgg caatcggacg tgatgattgt ctcgggcacg gtgacgtata aaatggcgcg
     3901 ggtggttcag cggctcttcg agcagatgcc cgagcccaag tgggtgattg ccatgggcgc
     3961 gtgcgcctcc accgggggga tgtaccgcag ctacgccgtg ttgcagggga tagaccggct
     4021 gctgccggtg gatgtgtatg tggggggctg cccgccccgc ccggaggcgc tgctggacgc
     4081 gctgatgaaa ctgcaggata aagtggccgg cgaaccgtcg ctggccacgc tgaagcgagt
     4141 ggcatgacgg cgcgggaact ggcactgcaa ctgggagccc aattccccgg cctggtctcc
     4201 gcgccggcgg agttccgggg tgaaatcacg ctgcgggtgg cggaggcgga gcggatgccc
     4261 gaggtgtgcg ccgcggccaa ggcggaactg ggctttgatt acctggtgga catgaccagc
     4321 gtggataact acggccagga cccgcgtttc accgtggtgt atcatcttta ctcctaccgc
     4381 caccattgct ggctgcggct gaagaccgcg gtgagcgagg aacagagcga actgcccacc
     4441 gtcaccggcg tctggcggac ggcggactgg catgagcggg agatttacga catgatgggc
     4501 ctgcgcttcc gcgggcaccc cgacctgcgg cgcatcctga tgtgggaggg ctacccttat
     4561 ttcccgctgc gcaaggattt ccccctggcc ggccggccca gcgacgtgcc ggaggtggcc
     4621 tttaccggcg ccgcgccgct ggagggcggg ccgtttgtga cgacggcggg gggcgcggat
     4681 gcggccgcgc gcgaaccccg ggcgcgcacc ccggaaagcg agccgcccag ggattaaaca
     4741 tggccgaggc gcacgagctt aagttcaagg acacggccgc gcgggcggcc gcggccgcgg
     4801 cgtgttccgc cccgccgcct gccgaggcgg aaccggggga tctggccggg gacaggatgg
     4861 ttctgaacgt ggggccttcg cacccggcca cgcacggggt gctgcgcctg gtgatcgaac
     4921 tggacggcga gcggattacc cgggtgcggc ccgagatcgg ctacctgcac cgcggcgacg
     4981 agaagatcgc cgagaattgc acctacaccc agttcattcc ctacacggac cggctggatt
     5041 acctggcgcc gatggccaac aacgtcgcct acgcgctggc ggtcgagaaa ctgctgggcc
     5101 tggagcggca actgccgccg cgctgccagt atatccgcgt gatctgcgcc gagctggcgc
     5161 gcctttcgtc gcatttgctg ggcatcggct gctacgcgat ggactgcggg gcgatgacgg
     5221 tcttcctcca taccttcacc gagcgcgaaa agatttacaa cctgtgcgag tcgctgacgg
     5281 gggcgcggtt caccaccacc tatacgcgca ttggggggct ggcgcgcgat ctgccgcccg
     5341 ggtgggtcga gcagtgccgg cagttccttg acgaggtggt ggtgaatatc gaggagacgg
     5401 agacgttcct gacccgcaac cgcattttta tggaccggac gcagggagtg ggagtgatcc
     5461 cgcgggaggt ggccatagat tacgggctga ccggccccaa cctgcgcgcc agcggcgtgg
     5521 cgcacgattt gcgcaaggcg cacccgtacc tggtgtatga ccaactgcaa tttgaagtgc
     5581 cggtgggcac ccggggcgat tgctacgatc gctaccgggt gcgcatggag gagatgcgcc
     5641 agagcgtgcg gatcgtgcgc cagtgcctgg accagttgcc ggacggcccc gtcaacgccc
     5701 ccgcggccaa ggtcctcctg ccgcccaaag cccgggtgct gacgaaaatg gaggaattga
     5761 tacaccagtt catgaacgtg acccaggggg tgaacgcccc gccgggcgag gtctatttcg
     5821 cgcatgagaa cccgaagggc gagctgggct tctacctgga cagccggggc ggcggcacgc
     5881 cttaccggtt gaaaatccgc tccccgtcct tcgtgaacct gagcatctta tcctggctgc
     5941 tgccgggcca cctgctgggg gacatcgtgg cggtgctggg gtcctttgat ttcgtaatgg
     6001 gagagtgtga ccgatgagtg agattcgtcc cctggaaccc attcccagcc cgctgcggga
     6061 gcagccggac tttgcggtgc cgcccgccct ggccgccgag ctggacgcgg tgatcgcgcg
     6121 gtatccccag gcccgcagcg catcgctgat gctgctgcac gcgatccagg agcagtttgg
     6181 ctatatctcc cggcaggcgg tggaatgggt ggcggcccgg ctggggctgc cgcccctcaa
     6241 catctacgaa ctggtgactt tctacccgat gttccacgcg gcgccggtgg ggaagtatca
     6301 tctcaagatc tgccgcacgc tgagctgcgc gctgggcggc tcgcaccaac tgcacgagct
     6361 ttgctgcacc cggctgggcc tggatccgcg ccagcacggg ccgcaaacca cgaaggatgg
     6421 caagttcacg gtcgagttcg tggagtgcct ggccggatgc ggcgccgcgc cggtgatgat
     6481 ggtcaacgac gcctttcacg aaggcgtcac gccgcggcgg gcggaggaga ttctgggagg
     6541 gtgctcatga ggacgcgttg cgctgccagc cggattcacc gcggggcgcg gggagggtgg
     6601 ctgcccgaca tggatttgaa ccatgacaaa cagatccaga gtctgctgtg ct
//
LOCUS       DAOPQG010000151         6558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_12886_length_6558_cov_13.609411, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000151 DAOPQG010000000
VERSION     DAOPQG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12886_length_6558_cov_13.609411"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PKI07_12890"
     CDS             complement(<1..213)
                     /locus_tag="PKI07_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51980.1"
                     /translation="MNPQYRIIGKGERRVDALGKVTGRAKYAADYNAGHQLWGKVLRA
                     EHPHARIVRVDGRLPCARTPVPSELAA"
     gene            complement(286..615)
                     /locus_tag="PKI07_12895"
     CDS             complement(286..615)
                     /locus_tag="PKI07_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51981.1"
                     /translation="MSGAKQFVTLAGMITITIANEGRFWPHYYYGPTTIQVTLASPNR
                     PLKQSPNCRYYEVIITEGPKYPGPKRRNLIFACKYNPHRNSLRFLKALFARISRADTP
                     ERAAGAE"
     gene            741..2057
                     /locus_tag="PKI07_12900"
     CDS             741..2057
                     /locus_tag="PKI07_12900"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC51982.1"
                     /translation="MMNIAAEVTRRRFLRQVATLSAAAGMAPMIVPAPVLGRGGALSP
                     SERITLGFIGVGSQGSGHLLGGAWTYVAGGYAGRKDVQVLAVCDVWCDRREKAARKVN
                     DHYAATRGQGGEKTCAAYNDFRQVLDRPDIDAVLIASPAHWHATMTIRAAEAGKDIYC
                     EKPTAVTVRESQAMLAAVRRYGRVYQAGTQQRSEYEGKFRRACEWVRSGRIGRLQAIY
                     AYRDGGAVCWPKRFGAAKPVPEGLDWDLYLGPAPWIPYDGDSSAHRFDLGELNWGQHH
                     YDIVQWAAGADDTGPVEIFLENDCSCYRYGNGVVVYGRAYPGEPVGIVGGACFVGTEG
                     RIAVDRDALVSDPPDLAREPLRPDETHLYRSHSHAGNFLECIRTRKRPICDADIAHRS
                     ASALLLGGVAKQLRRPLKWDPRAERFLKDDEANRLLSIAQRPPWRL"
     gene            2111..4573
                     /locus_tag="PKI07_12905"
     CDS             2111..4573
                     /locus_tag="PKI07_12905"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC51983.1"
                     /translation="MKSSPLFLLALGLLLSGARPAALAAPAPEEPRLIGVLRSDAPPA
                     EKDAACAQLKRIGTDRAIPALAALLPDEQLSHSARYALESMRSRKAGRALVKALARTS
                     GLTKAGIINSIGNRGETRAVAALAKLLADPEADIAAAVASALGQIGGAQALQALQAAA
                     ANTTGALHDACVDGCLRCADRLRAAGSRAQARAAFQHLYDTGKKDSIRTAAYRGLIQT
                     AGDQALPLITSALTGQDRACQSAALELARQVQAPEATRSLAALLPTVAPPMQAALIES
                     LAQREDAAAAPAILRLTDSPAPEVRVAALNALGILGGASAVPPLAAAAASGGEAQAAA
                     RQALIRLRRGQPTEMMLGLLPEARPEVQAELARALGNRRDLAAIPRLMELARQDASSA
                     SHAALLALAGLVDDAQMSAMVQFVVAAGSDRAREQAAEAVNAACHQILARRGRVNLEP
                     ISAALARGTPETRIALLPVCGGLIDPLARAALRAAVAAPDPPVRAAAIRALCDTPDPE
                     LLADALQIACEAPEENLRTLAIRACVRLTTQEEAIRLPRPEQIAPLKTILARPLRPEQ
                     QRAVLAGLAEIPDRETLALAEPMLDEAATQREAAQAIIKIASRLPCAQRIEATNVLAK
                     VRAVLTDADARKEAETLLKTIQADADYIIAWQVAGPYFQEGQDYKALFDLALPPENPD
                     AKEIQWRALPPDSDPKRPWVMDLLQALGGDQRVAYARTWIYSQSAQPARLDLGSDDGI
                     KVWLNHQVVHALNTFRGLEPDADKVKVNLRAGWNPLLLKITQLNQGWGFCARLRQPDG
                     TRLDGLKFSASPPAPAAARAGK"
     gene            complement(4580..5461)
                     /gene="hisG"
                     /locus_tag="PKI07_12910"
     CDS             complement(4580..5461)
                     /gene="hisG"
                     /locus_tag="PKI07_12910"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOC51984.1"
                     /translation="MKKKPVLRLGLPKGSLQEATIEKMGKAGFNISVSSRSYTPYVDD
                     EELEIRLIRAQEISRYVEHGYLDCGITGYDWIQENNSKVHEVGEFQFSKATRQSARWV
                     LAVPEHSPIKSVKDLQGKRIATEVVNLTRRYLRQHKVTAEVEFSWGATEIKAHELVDA
                     IVDVTETGSSLRANKLRIVDTLLSSTPRLIANRAAWKNAWKRRKIQTLALLLRGALDA
                     ETKVGLKMNIAQKNVTRLLQSLPALRNPTISQLGQKGWVAVETIIDKHIVRELIPALK
                     AAGAEGIIEYPLNKVVY"
     gene            complement(5629..6216)
                     /gene="purN"
                     /locus_tag="PKI07_12915"
     CDS             complement(5629..6216)
                     /gene="purN"
                     /locus_tag="PKI07_12915"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOC51985.1"
                     /translation="MAVAEACRAGAVPAEVGLVLSDVAEAGILEQARRRGIQAQFIPP
                     GKFRTRLDEEAEQAYLAALRAARVDLIVLAGFMRVLKPPFLDAFAGRIINIHPSLLPS
                     FPGLEAWKQALAHGVKVTGCTVHWVDAGVDSGPIIGQQAVPVLDEDTPETLHQRIQEA
                     EHALYPRCVGAIARGEIRLQGRRVFGGPPGVIRGA"
BASE COUNT         1110 a         2326 c         2029 g         1093 t
ORIGIN      
        1 cgcagcaagt tcggaaggca cgggcgttct ggcgcaaggg aggcggccat ccacgcgcac
       61 gatgcgggca tggggatgct ccgcgcgcag caccttgccc cagagctggt ggccggcatt
      121 gtaatccgcg gcgtatttcg cccgcccggt caccttgccg agagcgtcca cgcgccgttc
      181 tcctttgccg attatccgat actgcgggtt cacacggtct gtctggctgc ggtgctcaac
      241 gcgccaatgt tatgcaaacc tccggcaaaa gcaacgcccg gcgggttatt ccgcgccggc
      301 ggcccgctcg ggggtatccg cgcgggaaat gcgcgcaaag agcgccttca ggaaccgcag
      361 cgagttgcgg tgcggattat acttgcaggc gaaaatcaag ttgcgccgtt tgggcccggg
      421 atacttcggc ccttcggtga ttatgacttc ataataccgg cagttcggag attgctttaa
      481 cggccggttc gggctggcca gcgtcacctg aatggtcgtt ggcccatagt agtagtgcgg
      541 ccagaagcgg ccttcgttgg ctatcgtgat ggtaatcatc cccgccagtg tgacgaactg
      601 tttcgcgccc gacaagactg aacgggcgcg gcttcctcag tccgcgcggc gcgaattcat
      661 gcccggccgg ccttttcaca gcggtgccgc tcgacaaagc cggggccgac ggttaatctg
      721 cggcagccta attgcctgcg atgatgaaca ttgccgccga agtgacccgc cgccgttttc
      781 tgcgccaagt cgccaccctt tcagccgccg ccgggatggc gccgatgatt gtcccggcgc
      841 cggttctggg acggggcggg gctttgtctc ccagcgagcg catcaccctg ggattcattg
      901 gcgtgggcag ccagggaagc ggccatctgc tgggcggcgc ctggacgtat gtggcgggcg
      961 gctacgcggg gcgcaaagat gtccaggtgc tcgccgtttg cgatgtctgg tgcgaccgcc
     1021 gcgagaaggc ggcccgaaaa gtcaacgacc actacgccgc cacccgcggc cagggaggcg
     1081 agaaaacctg cgcggcctat aatgactttc gccaggtact ggaccgcccg gatattgacg
     1141 ccgtgctgat cgcctcgccg gcccactggc atgccaccat gaccatccgg gcggcggaag
     1201 cgggcaagga tatttactgc gaaaaaccaa ccgcggtgac ggtgcgcgag agccaggcga
     1261 tgctggccgc ggtgcggcgc tatgggcgcg tttaccaggc cggcacgcag cagcgcagcg
     1321 aatacgaggg caagtttcgc cgcgcctgcg aatgggtccg cagcggccgc attggcaggc
     1381 tgcaggccat ttacgcctat cgcgacggcg gggcggtgtg ctggccaaaa cggttcggcg
     1441 cggccaaacc cgtgcccgaa ggcctggact gggaccttta cctcggcccc gcgccctgga
     1501 ttccctacga cggagacagc agcgcccacc ggttcgacct tggcgaactc aactggggcc
     1561 agcaccacta cgacatcgtg caatgggccg cgggggcgga tgacaccggc ccggtggaaa
     1621 tcttcctgga gaacgattgc tcctgctacc ggtatggcaa cggcgtggtt gtctatggcc
     1681 gcgcctaccc gggagaaccc gtcggcatcg tgggcggggc ttgttttgtc ggcacggagg
     1741 ggcgcatcgc ggtggaccgg gatgcgctgg tctccgaccc gccggacctg gcgcgggagc
     1801 cgttgcggcc ggacgagacg cacctttacc gcagccacag ccacgcgggc aatttcctgg
     1861 agtgcatccg cacgcgcaag cgccccatct gcgacgcgga tatcgcccat cgctccgcca
     1921 gcgcccttct cctgggcggc gtggccaaac agttgcggcg ccccctcaaa tgggaccccc
     1981 gcgccgagcg gtttctcaag gatgacgagg ctaaccgcct gctctcgatc gcgcagcgtc
     2041 cgccctggcg tctctaatga atcaggtttc ccccggctgg cctttgactt tccgcttccc
     2101 caatccacgc atgaaatcct ccccgctctt ccttttggcg ctgggcctgc tgctttccgg
     2161 cgcccggccc gccgccctcg ccgcccccgc cccggaagaa ccgcgcctca tcggcgttct
     2221 gcggtcggat gcgccgccgg cggaaaaaga tgccgcctgc gcccaactca aacgcatcgg
     2281 caccgatcgc gccatcccgg ccctcgccgc gctgctgccc gacgagcaac tctctcactc
     2341 ggcccgctac gccctggaat ccatgcgctc ccgcaaggcc gggcgggcgt tggtcaaagc
     2401 cctggcccgc acgagcggac tgacgaaggc cgggatcatc aattccatcg gcaaccgcgg
     2461 cgaaacgcgc gccgtcgccg ccctggcgaa actgctggcc gatcccgaag ccgatatcgc
     2521 cgccgccgtc gccagcgcct tgggccagat cggcggcgcc caagccctcc aagctcttca
     2581 ggcggcagca gccaacacca ccggcgccct ccacgatgcg tgcgtggacg gctgcctgcg
     2641 ttgcgccgac cgcctgcggg cggccggttc ccgcgcccag gcccgcgccg ctttccaaca
     2701 cctctacgac accgggaaaa aggattccat ccgcacggcc gcgtatcgag ggctgattca
     2761 gacggcgggc gatcaagccc tgcccctgat aaccagcgcc ctcaccggcc aggaccgggc
     2821 ctgccagtcc gccgcgctcg aactggcgcg ccaggtgcaa gcgccggaag cgacccggtc
     2881 cctcgcggcg ctcctgccaa ccgtcgcccc gccgatgcag gccgcgctga tcgaaagcct
     2941 ggcgcagcgc gaagacgcgg cggcagcgcc cgccattctc cggttgaccg acagccccgc
     3001 gccggaagtg cgggtggccg cgttgaatgc gctcggcatt ctgggcggtg cctccgccgt
     3061 tcccccgttg gccgccgcgg cggcgtcggg cggagaagcc caggccgccg cccggcaggc
     3121 gctgatccgg ttgcggcgcg gccagcccac cgagatgatg ctgggcttgc tgccggaggc
     3181 ccggccggag gtgcaggccg aactggcccg cgcgctggga aatcgccgcg acctggccgc
     3241 gattccccgc ctgatggagc tggcgcggca ggacgcaagc tcggccagcc acgcggcgtt
     3301 gctggcgctg gccggcctgg tggacgacgc ccagatgagc gccatggtcc aatttgtcgt
     3361 cgcggcgggc agcgaccgcg cccgggagca agcggccgag gcggtcaacg ccgcctgcca
     3421 ccaaatcctg gcgcgccggg gccgggtgaa cctcgagccg atcagcgcgg cgctggcccg
     3481 cggcacgccc gagacccgca tcgccctgct gccggtctgc ggcgggctga ttgatcccct
     3541 ggcgcgcgcg gccctgcgcg cggcggtggc cgctccggac ccgccggtcc gcgccgcggc
     3601 catccgcgcc ctgtgcgaca cgccggatcc cgaactgctg gccgacgcgc tgcagattgc
     3661 ctgcgaagcg ccggaagaaa acctccgcac cctggcgatt cgcgcatgcg tccgcctcac
     3721 gacccaggag gaagccatcc ggctcccccg cccggagcaa atcgcgccgc tcaagacgat
     3781 cctggcccgg ccgctgcgcc cggagcagca gcgggccgtc ctggccggcc tggcggagat
     3841 tcccgaccgg gaaaccctcg cgctggcgga gccgatgctg gacgaggcgg ccacgcagcg
     3901 cgaagccgcg caggccatta tcaagatcgc atcccgcctg ccctgcgcgc agcgaatcga
     3961 ggcgaccaac gtcctagcca aagttcgcgc ggtactcacc gacgccgacg cgcggaagga
     4021 ggcggagaca ttgctcaaga ccattcaggc ggacgccgat tatatcattg cctggcaggt
     4081 cgccggcccc tatttccagg aaggccagga ctacaaggcc ctgtttgacc tggcgctgcc
     4141 gccggaaaat ccggacgcca aagagatcca atggcgggcg ctgccgcccg attccgaccc
     4201 gaagcgcccc tgggtcatgg acttgctcca ggccctgggc ggcgaccagc gcgtggcgta
     4261 cgcgcgcacc tggatttatt cccaatccgc gcaaccggcg cggctcgacc tcggctcgga
     4321 tgacggcatc aaggtctggc tcaaccacca ggtcgtgcat gcccttaata cctttcgcgg
     4381 tttggaaccc gatgccgaca aggtaaaagt caacctgcgg gcgggctgga accctctcct
     4441 gctgaagatt acgcaactga accagggctg gggcttttgc gcccgcctcc gccagcccga
     4501 cggcacccgc ctggacggcc tgaaattctc cgcgtcaccg cccgcgcccg ccgccgcccg
     4561 cgccgggaaa taacccgcct caatacacca ctttattcaa gggatactcg atgatcccct
     4621 ccgccccggc ggccttcagc gcgggaatca gctcgcggac aatgtgcttg tctatgatcg
     4681 tctcgactgc cacccagccc ttctgcccga gctgggaaat cgtcgggttg cgcaaagccg
     4741 gcagcgactg aagcagccgg gtcacgttct tctgcgcgat attcatcttc agtcccacct
     4801 tggtctccgc gtccagggcg ccccggagca gcagggccag ggtctgaatc ttccgccgct
     4861 tccaggcatt cttccaggcc gcccggttcg cgatgagccg cggcgtcgag gacagcagcg
     4921 tgtccacaat ccgcagcttg ttggcgcgca gcgaggagcc cgtctccgtg acatccacaa
     4981 tggcgtccac caactcgtgg gccttgatct cggtggcccc ccaggaaaac tcgacttccg
     5041 ccgtcacctt gtgctggcgc agataccgcc gggtgaggtt gacgacttcc gtggcgatgc
     5101 gcttgccttg caggtctttg accgatttga tcggcgaatg ctccggcacc gccagcaccc
     5161 agcgggcgga ttgccgcgtc gccttgctga actggaactc gcccacctcg tgcaccttcg
     5221 agttgttttc ctgaatccaa tcgtagccgg tgatgccgca atccaggtag ccatgctcga
     5281 cgtagcggct gatttcctgt gcgcgaatca gccgaatctc cagctcctcg tcgtccacat
     5341 agggggtgta ggatcggctg ctgacggaaa tattgaaccc ggccttgccc atcttctcga
     5401 tggtggcctc ttgcagactg cccttgggca atcccaaccg caacactggc ttcttcttca
     5461 tgctgttatt taatactcag gcgccggggg gcaaggtttt cgccgcattc atccgcaccc
     5521 gtgccgctta tttgccatga tttcgcgtct ccggcgacct ttttcttcgc tcacttcgcc
     5581 cgcgtttgcc gtaattggcc ggggcggctc cacggtttgg ctgcgaaatc aggcgccccg
     5641 aatcaccccg ggcggcccgc caaacacccg gcgcccctgg aggcggatct cgccgcgggc
     5701 aatcgccccg acgcagcgcg gatacaaagc gtgctccgcc tcctgaatcc gctggtggag
     5761 cgtctcgggc gtatcctcat ccagcaccgg cacggcctgt tggccgatga tcggccctga
     5821 atccaccccg gcgtccaccc aatgcacggt gcatccggta accttgaccc catgcgccaa
     5881 agcctgtttc caggcctcca ggccggggaa agacggcagc agcgaaggat ggatgttgat
     5941 gattcgcccc gcaaaggcat ccagaaatgg cggcttgagc acccgcataa accccgccag
     6001 cacaatcaaa tccacccgag ccgcgcgcaa ggccgccagg taagcctgct cggcttcctc
     6061 gtccagccgg gtccgaaact tgccgggcgg aatgaattgc gcctgaatcc cccgtcgtcg
     6121 cgcctgctcc aggattcccg cctccgccac atcgctcagc accagcccca cttccgccgg
     6181 tacagcgcca gcccggcagg cctcggccac ggccacaaag tttgaccccc gacccgaacc
     6241 cagcacgcct aagataaact tgcgctctcc actcacgccg ccttcttaca ggcaccgtgc
     6301 ccgccgactc aagccccaag tagcgcctat gaacgccaag aacgcatctc ggctcctgcc
     6361 agcctcccca aaccgggata tatgcgggtt ccaagccaga aatacgcgct atcttgacct
     6421 tttattagcc gaaacgattc cgttaagccg tcccaacgaa cctttcggtt ggtgaattca
     6481 ttggcataac cattgagttt ccaatccatt tcgccggtaa aatcaatagg ttaggcatag
     6541 agagggataa aatagaag
//
LOCUS       DAOPQG010000152         6481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_13072_length_6481_cov_17.328976, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000152 DAOPQG010000000
VERSION     DAOPQG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13072_length_6481_cov_17.328976"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..963
                     /locus_tag="PKI07_12920"
     CDS             109..963
                     /locus_tag="PKI07_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51986.1"
                     /translation="MDGAPTIFCFHASCAGAVAEANGRLRRELSASPWELRLPDGRTL
                     RSGDVLQAGGAVVAREAMKAAARAAGREAGEQLVLMTVRRLAAQFRADLFEFFHWPVA
                     EVLEDSPLAVSERDAEDQFRTWLKLWPACSTVWIGDVYSSGKPEHRTHFRPVGEWYQI
                     GPVMGNFTCGSSFKPGSFSRSNENLTGHRFMVVESDTLSKDEVGAVFAYLRRRLRYPL
                     HCIIDTAGKSLHGWFDAPRSKMLENRLKAGLEEFGCDPKVFTYSQPVRVPGAFRDGKL
                     QKLVWLRH"
     gene            965..2011
                     /locus_tag="PKI07_12925"
     CDS             965..2011
                     /locus_tag="PKI07_12925"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC51987.1"
                     /translation="MSEPLDIAVDLGAVPEPLPEILNLRQLVETAPAPPPQIIEGVLH
                     KGCKMVLGGTSKSNKSWCLLDLAVSVASGQEWWGRRCEKMPVVYINFELHVWAIAQRL
                     NALCGARPECKGMGDTLHVWNLRGHNSDLTLLRPKLEEQLARGVAPCQEFGLIILDPA
                     YKVLGDRDENANGEIASLMNELEGLARNSGAAVVVAHHFAKGDSASKSAIDRMSGAGA
                     WARDPDSILVLTPHEEADCFTVSAILRNLPQVDEFVVRWNYPRMTVADDLNPEALRRP
                     QGRSKVCTDLEFVGTVLGKESFNYQKVIKRAREFLGMSEASSNRYLTRLKAAGLVCHS
                     GGLYWAAHQEVTPE"
     gene            2162..2785
                     /locus_tag="PKI07_12930"
     CDS             2162..2785
                     /locus_tag="PKI07_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51988.1"
                     /translation="MPLLFEQQEKESDKAFAAFSAYLNMGPQRSYEAVSTKLRKSVGL
                     LRRWGKRFDWPERIKAHTAHLVVIEREAVEVVARGKAVEWGKRQQELREEEWELHGEC
                     IKAAREALKRFYERGKGATLGDIARLLDLASKLGRLSTGMATDRTEVTGDGGGPVRVE
                     FELALKKIYGQAEPGPIVDVETVPPALPAGPHSSREGGTPARQGGAE"
     gene            2782..4164
                     /locus_tag="PKI07_12935"
     CDS             2782..4164
                     /locus_tag="PKI07_12935"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HOC51989.1"
                     /translation="MSPDEIYLAAAKAAGCPADQMRNFERAGVLLQPRQLAASGAARL
                     CDRAGGPTAVGYGGARGGGKSHWLLAQMGADDCQRVPGLKCLLLRKVGKANMEHFEDL
                     RRRLFGQLTHEFSAYRGVMTFNNGSRIIAGHFQAEKDIDAYLGLEYDVIGIEEATTLT
                     SRKHQDITTCCRTSKPNWRPRIYSTTNPGGIGHAWYRTRFVLPYLEKRECETRFVPAR
                     VVDNQFNNPEYRKVLEGLSGWQKRAWLDGDWDIAAGQFFTTFRRDVHVVRDFDESRAR
                     EWFAALDYGFTHYTAVVLGCTDGDGNVFVVDEHAERVWLPQRHAPAIAAMLARHGLAV
                     EQLRRFVAGADVFSRQSDGSTVASQYGKLGITLRPASMDRVSGWAEVLHRLGDPANGV
                     QPRLFIHERCARLAECLPALQHDPNRPEDVLKVDCDEDGIGGDDAADALRYLVATKAR
                     SVAQRKLRGL"
     gene            complement(4694..5098)
                     /locus_tag="PKI07_12940"
     CDS             complement(4694..5098)
                     /locus_tag="PKI07_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51990.1"
                     /translation="MKSPITNPDNSPVIYTYTRSHAIADGFQIDVSKVAAEAGIRFPV
                     FITRAVYEQCVAVPPGVTGQDESGRLWDVVWMLRFAIIRSRPGTSRLTVALYVRNSNQ
                     HPARLTKLIATAGAVDVDDPAPAITVMLPDED"
     gene            complement(5150..5629)
                     /locus_tag="PKI07_12945"
     CDS             complement(5150..5629)
                     /locus_tag="PKI07_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51991.1"
                     /translation="MKTKSNKKEKTNSGLSFRPEQSSGENNNTSSTGKTFTEAMAEVD
                     AQKHADKKPTRILPYVNYQLREENRALPTEQVLDLLRKWVPQAYDLAEVVGKWVWITF
                     PKPPTEQLRGQLSQFGFHWNNLRKCWQHPCGQFTTEGSGADPRTKYGSHFAADLKAA"
BASE COUNT         1213 a         1643 c         2400 g         1225 t
ORIGIN      
        1 ttcgcgccgc ccaagtctct cataacacct ggctcaattt aaggggagct ttccacgggc
       61 gaggcgctgc acaccagcgg caacggcaag agggattgcc gggtgaatgt ggacggagcg
      121 ccaaccatct tctgctttca tgcgtcgtgt gcgggcgctg tggcggaagc caacggacgg
      181 ctgcgccggg agttgagcgc ctcgccgtgg gaactgcggc tgccggatgg cagaaccttg
      241 cggagcggcg atgtgctcca ggccgggggg gcggtcgtgg cgcgcgaggc catgaaagcg
      301 gcggcgcggg ccgcaggccg cgaagcgggc gagcaattgg tattgatgac ggtgcggcgg
      361 cttgccgcgc agttccgggc ggacttgttt gagttcttcc attggccggt ggccgaggtc
      421 ctcgaagatt cgccgctggc ggtgagcgag cgcgatgccg aagaccagtt ccggacgtgg
      481 ctgaagctgt ggccggcctg ctcgacagtg tggatcgggg atgtgtatag ctcgggcaaa
      541 ccggagcacc gaactcattt ccgtccggtt ggagagtggt atcagatagg gccggtgatg
      601 ggaaatttca catgcgggtc ctcatttaaa ccggggagtt ttagccgctc caatgagaac
      661 ctgaccgggc accggttcat ggtggtggag tctgacaccc tgagcaagga cgaggttgga
      721 gcggtgtttg cttacctgcg gcggcggctg cgctacccgc tgcactgcat catcgacacg
      781 gccggcaagt ccttgcatgg gtggtttgat gcgccgcgga gcaagatgtt ggaaaaccgg
      841 ctgaaagccg gtctggaaga gttcgggtgc gacccgaaag tgtttaccta ctcacagccc
      901 gtgcgggtgc ccggagcgtt cagggatggg aaactgcaaa agctggtctg gttaaggcat
      961 tgacgtgagc gaaccgttgg acatcgcagt ggacctgggc gcggtgcctg agccgctgcc
     1021 ggagattttg aaccttcggc agttggtgga aacagcgccc gccccaccgc cacagataat
     1081 tgagggcgtg ctgcacaagg gctgcaaaat ggtcctgggc gggacgagca agagcaacaa
     1141 gagttggtgc ctgctggacc tggcggtaag cgtggccagc gggcaggaat ggtgggggcg
     1201 gcgctgcgag aaaatgcctg tggtgtatat caacttcgaa ctgcacgtat gggccatcgc
     1261 acagcggtta aatgcgttat gcggtgcgcg tcctgaatgc aaaggcatgg gggatacgct
     1321 gcacgtgtgg aacctgcggg gacataactc ggacctgaca ttgcttcggc caaagctcga
     1381 agagcaactg gcccggggag tggctccctg ccaagagttt gggctgatca tcctggaccc
     1441 ggcgtacaag gtgctgggtg accgggacga gaacgccaat ggcgaaatcg ccagcctgat
     1501 gaacgagctt gagggcctgg cccggaactc gggcgcggca gtggtcgtag ctcatcactt
     1561 cgccaagggc gacagcgcga gcaagagcgc cattgaccga atgagcggag cgggtgcctg
     1621 ggcgcgtgat ccggattcaa ttctggtgct tactccgcat gaagaggcgg attgtttcac
     1681 ggtgagcgcc atcctgcgca acctgccaca agttgatgaa ttcgtggtgc ggtggaatta
     1741 cccgcgcatg acggtagccg atgacctaaa tccggaggcg ctgaggcggc cgcagggccg
     1801 aagcaaggtc tgcacggacc ttgagttcgt ggggacggtg ctgggaaaag agagcttcaa
     1861 ctatcaaaag gtaattaagc gggcgcgaga gtttttgggc atgagtgagg ccagcagtaa
     1921 ccgatatttg acccgtttga aggcggctgg gctggtgtgc cactccggcg ggctgtattg
     1981 ggctgcgcat caagaggtta cgccggaatg agggcgttac tctcaaaact ctcacaaccc
     2041 tatagtgata tgagagagag aaccaaaagg caggcagttg ggaaggaacc accctccttg
     2101 gggtcgggtg gtccttccct cactgccatt aggctgagag ttgttctgaa aggagggctg
     2161 tatgccgctg ctatttgagc aacaggagaa ggaaagcgac aaggcgttcg ccgcctttag
     2221 cgcgtatctg aatatggggc cgcaacggtc gtatgaggcg gtctcgacga agttgcgaaa
     2281 gagtgtaggg cttctcagaa ggtgggggaa gaggtttgac tggccggagc ggatcaaggc
     2341 gcatacggcg cacctggttg tcatcgagcg tgaggccgtt gaagtcgtgg cgcgagggaa
     2401 ggctgtggag tggggaaagc ggcagcagga attgcgcgag gaggaatggg aactgcatgg
     2461 tgagtgcatc aaggcggcgc gtgaggcgct gaaacggttt tatgagcggg gcaagggtgc
     2521 gaccctgggc gacatcgccc ggctgctgga cctggcgtcg aagctcgggc ggctttcgac
     2581 ggggatggcg accgaccgaa cagaagtgac gggcgatggc ggcgggccgg tgcgggtgga
     2641 gttcgagttg gcgctaaaga agatttacgg gcaggcggag cctggcccca ttgttgatgt
     2701 cgagaccgtg ccccccgcgc tgcctgcggg tccgcattcg tcccgcgagg gcgggactcc
     2761 ggcgcgacag ggaggtgcgg agtgagtccg gatgagattt acctggcggc agccaaggct
     2821 gccggatgcc cggctgacca gatgcggaac tttgagcggg cgggtgtgct gctccagccg
     2881 cggcagcttg cggcctcggg ggcggcaagg ctgtgcgacc gggcgggcgg gccgacggcg
     2941 gtcggctacg gcggggcgcg tggcggcggg aagagtcatt ggctgcttgc acagatggga
     3001 gccgatgatt gccagcgtgt gccggggctg aaatgcctgc tgctgcggaa ggtgggcaag
     3061 gcaaacatgg agcatttcga ggatctgagg cgtcggctgt tcggtcaact cacgcatgag
     3121 ttctcggcgt atcgtggggt gatgaccttc aacaacgggt cgcgaatcat cgcgggtcac
     3181 ttccaggcgg agaaggacat tgatgcttac cttgggctgg aatacgacgt gatcgggatc
     3241 gaggaggcga cgacgttgac cagtcggaag catcaggaca ttacgacgtg ctgtcgaacg
     3301 agcaagccga actggcgtcc tcggatatat tcgacgacca atcccggagg gattgggcat
     3361 gcgtggtatc ggacgaggtt tgtgctgccc tacctggaaa agcgcgaatg cgaaacgcgg
     3421 ttcgtcccgg cgcgtgtggt ggataaccag ttcaacaatc ccgaataccg gaaggtgctc
     3481 gagggtctga gcggctggca gaagcgggcc tggctggatg gggactggga cattgcagcg
     3541 gggcagtttt tcacgacgtt ccggcgggat gtgcatgtgg tgcgagactt cgatgaaagc
     3601 cgagcgcggg aatggttcgc cgccctggac tacgggttca cgcattacac agcggtagtg
     3661 ctgggctgca cggatggcga cggcaacgtg ttcgtggtgg acgaacacgc ggagcgggtg
     3721 tggctgccgc agcggcacgc gccagccatc gccgcgatgt tggcgcggca cggtctggcc
     3781 gtggagcagc ttcggcggtt tgtggccggg gcggatgtgt tcagccggca gagcgatggc
     3841 agcacggtgg caagccagta tgggaagctt ggcataacgc tgcggcctgc gagcatggac
     3901 cgggtaagcg ggtgggctga ggtgctgcac cggctaggcg accctgcgaa cggggtgcaa
     3961 cctcgtctgt tcatccatga acggtgtgcg cggttggcgg agtgtctgcc tgcgctccag
     4021 catgatccga accgtccgga ggatgtgctg aaggtggact gtgatgaaga cggcatcggc
     4081 ggcgatgacg ccgccgatgc gctgcggtat ctggtggcga cgaaggcgcg aagcgtggcg
     4141 caacggaagt tgcgcgggct ttgaaagcga agaccccttg gggacggaag gggtctagca
     4201 ttggggatgg gtgggtggga gcgaacacga gtagcaggcg gaatgacggc gcgatgactg
     4261 cctgccttct tccgtttgca ggcagcgtga cggcgtgagg cttgcggggg gagtgagcgg
     4321 cttggggatg gtccgcgcgc gcctaaacac ccggcacccg gagctaccta gcagcacgca
     4381 tgacggcgct gatcttggca cctgcaaggg ctgtgacggc gtgagggctg cggagcggag
     4441 gtcgtctcga atttgcgcgc gcggcggaag cggagtccag aggatatatc ctttggcgca
     4501 cgggcctggg acggcgtgag tcccagaaaa aaataatggc gggtgcaggg ctgccaagtc
     4561 ctgccgggtc cagggcggag ccctggccgc cggagggtcc gagccggacg ggccaacggg
     4621 ggccaagcgc cggcaggcag agagatgtgc cgcacaatcc cgggtgagcg ggaggcgcct
     4681 ggccccaagg gggtcaatct tcgtcgggca acatgacggt gatggccggg gccgggtcgt
     4741 ccacgtccac cgcgccagcg gtggcgatca gcttggtgag gcgggccggg tgttggttgg
     4801 agttgcgcac gtagagtgca accgttaagc ggctggtgcc tggtcggctg cgtatgatgg
     4861 cgaagcgcag catccaaacg acatcccaca ggcggccgga ttcatcctgg ccggtaactc
     4921 cggggggaac ggcaacgcat tgctcataaa ctgcgcgagt gatgaacacc gggaagcgga
     4981 taccggcttc ggcggcaacc tttgaaacgt cgatttggaa gccatcagcg atggcgtgcg
     5041 agcgggtgta ggtgtagatg acgggggagt tatcgggatt tgtaattgga gatttcatat
     5101 ttgagaagtg gaagcgggcg cagccctgag ggctgcgccc tgggttgaat caagcggctt
     5161 tgaggtctgc ggcgaagtgg ctgccgtatt ttgtgcgggg atcggctccg ctgccttcgg
     5221 tagtgaactg gccgcagggg tgctgccagc atttgcggag gttgttccag tgaaagccga
     5281 actgtgacaa ctggccgcgc aactgctcgg tggggggctt ggggaaggta atccagaccc
     5341 acttgccaac gacttcggcg aggtcgtaag cctggggaac ccacttgcgc agcaggtcga
     5401 gaacctgctc ggtgggcagt gcgcggtttt cttcgcgcaa ctggtagttc acgtacggga
     5461 gaatgcgggt gggttttttg tcggcgtgtt tctgtgcgtc aacctcggcc attgcctctg
     5521 taaaggtctt gcctgtgctg ctggtgttgt tgttttctcc gctcgattgc tcgggacgga
     5581 agctgagccc tgaattagtt ttttcttttt tatttgattt ggttttcatg gtgttttttt
     5641 tgtgctctgc tgctcggtcc ggtctttcgc cctttcaccc aaaaagccca cgtaatcagg
     5701 gcggcaggcg ggagtagtct tcagagggca ccgccttttg gccccctggt gaagtgggct
     5761 tgtgaaaggg cgagagactg gacccccgtt cagcagagga agcacaaagc ggcaaaacac
     5821 ctgaaaacgc ggatgtttcg catagcgccg caggcattgg ggagtccaga gggtggaaac
     5881 cctttggcgg gggtctgggg cgcgaagccc cgggctgtta atcgtctgct ttccgcgcgc
     5941 gcctgatgtt gttttgaagt gtagcggaga gaggggctgg catgaaggat gcaggagcgg
     6001 ttggcgcggg cactgcaact ggcattgggg cgcaggggcc agtttagcag tgaatgtgcc
     6061 aaccgctcca ggcggcagga cacaagacgc tgttatcttc aatgcaccgg gccgccgaag
     6121 ggccgagtgg gttgcgcctg cggcaggagg ccttgcgatg aggcggcggg gtccccaatg
     6181 ccgagggttc acgcgccagc gggatacggc attggggcgg cattgcagat aacagctctt
     6241 gtgtctggaa ccggaagcat ccttcatgac agccccgaac gaagcgaagt ccaatctggc
     6301 gcgcgcggtg tttgcctccg agccacgctc acggggtctt gcgccggcct gctggctttt
     6361 gcagcaggcg ggcgctttcc ctcagccgcc agtctgcgag tctcagcggg acccgccatg
     6421 cgggtccagc ccgagtcagt ccgggctgag ctgagggctc gcagtctggc ggcggtcgta
     6481 t
//
LOCUS       DAOPQG010000153         6353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_13402_length_6353_cov_12.087171, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000153 DAOPQG010000000
VERSION     DAOPQG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13402_length_6353_cov_12.087171"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1085
                     /locus_tag="PKI07_12950"
     CDS             159..1085
                     /locus_tag="PKI07_12950"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC51992.1"
                     /translation="MLLRLGCYAGSLLALLGGAGAFGAETFRVATYNVENYLDAPTET
                     RSPKPAAARAKVCESILALKPDVLAVQEMGRPSALLELRDALKAGGLDLPHWEHVAGW
                     DTNIFVAVLSRFPFAARRSHTNENFLLHGRRFHVSRGFAEVDIQVNPRYTLTLLVTHL
                     KSKRPLPDADEAELRLAEARLLRQKIDDRFAANPDINLVVAGDFNDTKESPSTRAVIG
                     RGRFKLVDTRPAERNGDNTPSPNPAWDPRNVTWTHFYGKTDSYSRLDFLLLSPGLARE
                     WLPDQTYVLTLPNWGVGSDHRPVVATFTAADR"
     gene            1091..2431
                     /gene="gdhA"
                     /locus_tag="PKI07_12955"
     CDS             1091..2431
                     /gene="gdhA"
                     /locus_tag="PKI07_12955"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOC51993.1"
                     /translation="MHPYIAEVLEQVARRNAGEPEFLQAAREVLESLAPVFDRHRHYR
                     DAQILERIVEPERQILFRVPWQDDTGRLRVNRGFRVQFNSALGPFKGGLRFHPSVNLG
                     ILKFLGFEQIFKNALTTLPMGGAKGGSDFDPKGKSPHEVMRFCQSFMTGLFRHLGPNT
                     DVPAGDIGVGAREIGYLFGQYKRLQNEFTGTLTGKGLNWGGSLIRPEATGYGAVYFAE
                     EMLKTRRESLAGKTCAVSGSGNVAQYTIEKLLQRGARPVTLSDSEGAIHDPDGLNAEK
                     LAWVLELKNHRRGRIKEYAPRFGARYFPGQRPWDIPCDCAFPCATENEISGADARTLL
                     NHGCTLVSEGANMPSAPEAIDLFQQAGILYGPGKAANAGGVATSGLEMTQNAMRLAWS
                     REEVDQRLHRIMVDIHHLCRQTAEDYGHPGNYVMGANIAGFVRVADAMLDHGLV"
     gene            2466..5024
                     /locus_tag="PKI07_12960"
     CDS             2466..5024
                     /locus_tag="PKI07_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOC51994.1"
                     /translation="MPHPLPATTGLPGLDAVLSGIEPGDSLVWEVDTIADYQEFVAPY
                     AAAAAAAASRRIYFRFASHPPLLPPGAGFELHPLTPARGFEALMRETHAVIESAPPGA
                     RYVFDCLSDLAEHWLSDQSLGNFFHLVCPRLAALGAVAYFALYRDRHSAYALEPIHRA
                     TRYQLDVFRLEGRLYIRPVKVQHRSRDVMNTLHVRSADRFLPVRESAVLASILARTQW
                     PRLQGDRRAGHWRILFREAEAAAADARAGRAPPGRADDLLRQLRRALRVHRSGIAGLV
                     ERYLTLDDFLALRDRMIGIGSVGGKTLGMLVARAIIRAHDPELARRLEAHDSFFVGAE
                     VFVTFLVQNDVWWLREQQKNPATFLQGLAEGRRRIQTGHFPPAILEQFQGLLDYFGES
                     PYIVRSSSILEDARGNAFSGKYESVFVANRGSREQRRQGLLDAVRRVYASVFDEQALL
                     YRQRRGLLDSEERMALLIMRVSGAPNGRYYYPQAAGVGLSFNPYAWHPDILPQAGVLR
                     LVFGLGTRAVDRSDDDYTRLVALNAPARRPEADFDDVREHSQRRMDCLDLQDDKFVSL
                     PFEEVIEAGADFPLDLYLTRAARDYPLITFDRLLRETPVARDLRRMLELLEAAYQHPV
                     DIEFTLNFLGDGSYRIHLLQCRTFQIRRPGPAPAPPARPGADPLLAAHGAVIGVSREQ
                     PLSRIIYVATDRYAALPERDRYAVARLIGRLNRLHPPADRGLMLIGPGRWGTLSPALG
                     IPVSFAEISRASVVCEIVAMHENLIPDVSLGTHFFNDVVEHDMLYLAYFPNKTGNRLH
                     DDWFRAAPNRLLHLEPDAARLAGVVRVIDCADAHPRVWLWADTTEQKAAVFTRP"
     gene            5087..6169
                     /locus_tag="PKI07_12965"
     CDS             5087..6169
                     /locus_tag="PKI07_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HOC51995.1"
                     /translation="MPRKTLTPAPPPPPRTLALPAALAGALLLPARAQVDIQPAVETE
                     AVAGDADDPAVWVNRADPARSRILGTDKGGALYVFGLDGRIRQKISGLQRPNNVDVEY
                     GFPRNGQAIDIAVLTEARAHRLRIYEISPAGQLTDISSPHTTVFDGQAQPMGIALYRR
                     PADGVIYALVSKKTDPDHRILGQYQLLGDPDGKVRLKEIRRFGRFSGAGTVEAVAVDD
                     ELGWVYYADESTGIRKYPADPDHPAASRELALFGTDEYSADREGIALYPQPRGRGCLI
                     CTDQLKNSRYLIYPRAGPPENPHQHGRAIKIIRGGADNTDGIEVVSEPLGPRFPQGIL
                     VAMNSKGRNFKIYDWRDIAQTGKPKL"
     gene            complement(6166..>6353)
                     /locus_tag="PKI07_12970"
     CDS             complement(6166..>6353)
                     /locus_tag="PKI07_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51996.1"
                     /translation="SWRSADAAERRDRWRGYPFPSEEAGGVTGGLRLRAGVAVSARRA
                     AGFFRGGRGAAGWEGRV"
BASE COUNT         1066 a         2772 c         1636 g          879 t
ORIGIN      
        1 ttcctataat gactcggctt gcatagtggt gttatgccgg tcgaggaacc gttacccccc
       61 tggcttaagg acagtgtcaa gatgcaccca ttcgcccgga accgccgcaa aatcttttga
      121 cacgcccgcc ggggcggcgt attagcgtga actggattat gttattacgc cttggttgct
      181 atgcggggtc cctgctggcg ctgctgggcg gcgccggcgc gttcggggcc gaaaccttcc
      241 gcgtcgccac ctacaacgtg gaaaactatc tcgacgcgcc caccgaaacc cgctccccca
      301 aacccgccgc cgcccgcgcc aaggtgtgcg aaagcatcct cgccctcaaa cccgacgtgc
      361 tcgccgtgca ggaaatgggc cgccccagcg ccctgctcga actgcgcgac gccctcaaag
      421 ccggcggcct cgacctgccc cactgggagc acgtcgcggg ctgggacacc aacatcttcg
      481 tcgccgtcct cagccgcttt cccttcgccg cccgccgttc gcacaccaac gaaaacttcc
      541 tgctccacgg ccgccgcttc cacgtcagcc gcggctttgc cgaggtggac atccaggtca
      601 acccccgcta caccctcacc ctcctggtca cgcacctcaa atccaaacgc cccctccccg
      661 acgccgacga agcggagctg cgcctggccg aagcccggct gctgcgccag aagattgacg
      721 accgcttcgc cgccaacccc gacatcaacc tcgtggtggc gggcgacttc aacgacacca
      781 aggaatcccc ctccacccgg gcggtgatcg gccggggccg cttcaagctg gtggacacgc
      841 gcccggccga gcgcaacggc gacaacaccc ccagccccaa ccccgcctgg gacccgcgca
      901 acgtcacctg gacgcatttc tacggcaaaa ccgacagcta cagccgcctg gatttcctcc
      961 tgctcagccc cggcctcgcg cgcgaatggc tccccgacca gacctacgtc ctcaccctcc
     1021 ccaactgggg cgtcggctcc gaccaccgcc ccgtggtggc caccttcacc gccgccgacc
     1081 gataaatccc atgcacccct atatcgccga agtcctggaa caggtggcgc gccgcaatgc
     1141 cggcgagccg gagttcctcc aagccgcgcg cgaggtgctc gaatcgctcg cgcccgtgtt
     1201 cgaccgccac cgccactacc gcgacgcgca aatcctcgaa cgcatcgtcg agccggaacg
     1261 ccagattctc ttccgcgtcc cctggcagga cgacaccggc cggctgcgcg tcaaccgcgg
     1321 cttccgggtg cagttcaaca gcgccctcgg ccccttcaaa ggcgggctgc gcttccaccc
     1381 cagcgtcaac ctcggcatcc tcaagttcct cggcttcgag caaatcttca aaaacgcgct
     1441 gaccaccctg cccatgggcg gcgccaaagg cgggtcggac tttgacccca aaggcaaatc
     1501 cccccacgaa gtcatgcgct tctgccagag cttcatgacc ggcctgttcc gccacctcgg
     1561 ccccaacacc gatgtccccg ccggcgacat cggcgtgggc gctcgcgaga tcggctacct
     1621 gttcggccag tacaaacgcc tccaaaacga gttcaccggc acgctcaccg gcaagggcct
     1681 caactggggc ggctcgctca tccgccccga agccaccggc tacggcgcgg tttatttcgc
     1741 cgaggaaatg ctcaaaaccc gccgcgaatc gctcgcgggc aaaacctgcg ccgtctccgg
     1801 ctcgggcaac gtcgcgcaat acaccatcga aaaactgctc cagcgcggcg cccgcccggt
     1861 gaccctctcc gattccgaag gcgccatcca cgacccggac ggcctcaacg ccgaaaaact
     1921 ggcctgggtg ctcgaactca aaaaccaccg ccgcggccgc atcaaggaat acgccccgcg
     1981 cttcggcgcc cgctacttcc ccggccagcg cccctgggac atcccctgcg actgcgcctt
     2041 cccctgcgcc accgaaaatg aaatctccgg cgccgacgcc cgcaccctcc tcaaccacgg
     2101 ctgcaccctg gtgtccgagg gcgccaacat gccctccgcc cccgaagcca ttgacctgtt
     2161 ccagcaagcc ggcatcctct acggccccgg caaagccgcc aacgccggcg gcgtcgccac
     2221 ctccggcctg gaaatgaccc aaaacgccat gcggctggcc tggagccgcg aagaagtgga
     2281 ccagcgcctg caccgcatca tggtggacat ccaccacctc tgccgccaga ccgccgaaga
     2341 ctacggccac cccggcaact acgtcatggg cgccaacatc gccggcttcg tccgcgtcgc
     2401 cgacgccatg ctcgaccacg gcctggtcta aacccgcccc ccggccccga aggcggccgc
     2461 tccccatgcc gcatcccctc cccgccacca ccggcctgcc gggtctggac gccgtgctca
     2521 gcggcatcga gccgggcgac agcctcgtct gggaggtgga caccatcgcc gattaccagg
     2581 aattcgtggc cccctacgcc gccgccgccg ccgccgccgc cagccgccga atctatttcc
     2641 gcttcgccag ccacccgccg ctgctccccc ccggcgccgg cttcgagctc caccccctga
     2701 cccccgcccg cggcttcgaa gccctgatgc gcgaaaccca cgccgtgatc gaatccgccc
     2761 cccccggcgc gcgctatgtc ttcgactgcc tgtcggacct ggccgaacac tggctgtcgg
     2821 accaaagcct cggcaacttc ttccacctgg tttgcccgcg cctggccgcg ctcggggcgg
     2881 tggcctactt cgccctctac cgcgaccgcc attccgccta cgcgctcgag cccatccacc
     2941 gcgccacccg ctaccagctc gacgtgttcc gcctcgaagg ccgcctctac atccgccccg
     3001 tcaaagtcca gcaccgctcc cgcgacgtga tgaacacgct ccacgtccgc agcgccgacc
     3061 gcttcctccc ggtccgggaa agcgccgtcc tcgcctccat cctcgcccgc acccagtggc
     3121 cgcgcctcca gggcgaccgc cgcgccggcc actggcggat cctgttccgc gaagccgagg
     3181 ccgccgccgc cgacgcccgc gccggccgcg ccccgcccgg gcgcgcggac gacctgctgc
     3241 gccagctccg ccgcgccctg cgcgtgcatc gctccggcat cgccggcctg gtcgaacgct
     3301 acctcaccct cgacgacttc ctggccctcc gcgaccgcat gatcggcatc ggctccgtcg
     3361 gcggcaaaac cctcggcatg ctcgtcgccc gcgccatcat ccgcgcgcac gaccccgagc
     3421 tggcccgccg cctggaggcg cacgactcgt tcttcgtggg cgccgaggtg ttcgtcacct
     3481 tcctggtgca aaacgatgtc tggtggctgc gcgaacagca gaaaaacccc gccaccttcc
     3541 tccagggcct ggccgagggc cgccgccgca tccaaaccgg ccacttcccc cccgccatcc
     3601 tcgaacaatt ccagggcctg ctggactact tcggcgaatc cccctacatc gtccgctcca
     3661 gctccatcct cgaagacgcc cgcggcaacg ccttctccgg caaatacgaa agcgtcttcg
     3721 tcgccaaccg cggctcgcgc gagcagcgcc gccagggcct gctggacgcc gtccgccgcg
     3781 tctatgccag cgtcttcgac gagcaggccc tcctctaccg ccagcggcgc ggcctgctcg
     3841 acagcgagga acgcatggcc ctgctcatca tgcgcgtcag cggcgccccc aacggccgct
     3901 actactaccc ccaggccgcc ggcgtcggcc tctccttcaa cccctacgcc tggcacccgg
     3961 acatcctccc ccaggccggc gtcctgcgcc tggtcttcgg cctcggcacc cgcgccgtgg
     4021 accgcagcga cgacgactac acccgcctgg tcgcgctcaa cgcccccgcc cgccgccccg
     4081 aagccgactt cgacgacgtg cgcgagcact cccagcgccg catggattgc ctcgacctgc
     4141 aggacgacaa attcgtctcc ctgccctttg aagaagtcat cgaagccggc gccgacttcc
     4201 ccctcgacct ctacctcacc cgcgccgccc gcgattaccc cctgatcacc ttcgaccgcc
     4261 tgctgcgcga aacccccgtc gcgcgcgacc tgcgccggat gctcgaactg ctcgaagccg
     4321 cctaccagca ccccgtggac atcgagttca ccctcaactt cctcggcgac ggctcctacc
     4381 gcatccacct cctccagtgc cgcacctttc aaatccgccg ccccggcccc gccccggccc
     4441 ccccggcccg ccccggcgcc gaccccctcc tggccgccca cggcgccgtc atcggcgtct
     4501 cccgcgagca acccctctcc cgcatcatct acgtcgccac cgaccgctac gccgccctgc
     4561 ccgaacgcga ccgctacgcc gtcgcccgcc tcatcggccg cctcaaccgc ctccaccccc
     4621 ccgccgaccg cggcctcatg ctcatcggcc ccggccgctg gggaaccctc agccccgccc
     4681 tcggcatccc cgtctccttt gccgaaatct cccgcgcctc cgtcgtctgc gaaatcgtcg
     4741 ccatgcacga aaacctcatc cccgacgtct ccctcggcac ccacttcttc aacgacgtcg
     4801 tcgaacacga catgctctac ctggcctact tccccaacaa aaccggcaac cgcctccacg
     4861 acgactggtt ccgcgccgcc cccaaccgcc tcctccacct cgaacccgac gccgcccgcc
     4921 tggccggcgt cgtccgcgtc attgactgcg ccgacgccca cccgcgcgtc tggctctggg
     4981 ccgacaccac cgaacaaaaa gccgccgtct tcacccgccc ctgaaccgcc ccccgccccc
     5041 gccgccgccc attgccaccc gcgccccaac cggataccct cgccccatgc ctcgtaaaac
     5101 gctgaccccg gccccccccc ccccgccccg aaccctcgcc ctccccgccg ccctcgcggg
     5161 cgcgctcctc ctccccgccc gcgcccaggt ggacattcaa cccgccgtcg aaaccgaggc
     5221 ggtggcaggg gacgccgacg accccgccgt gtgggtcaac cgcgccgacc cggcccgcag
     5281 ccgcatcctc ggcaccgaca aaggcggcgc gctctatgtc ttcgggctgg acggccggat
     5341 ccggcagaaa atctccggcc tccagcgccc caacaacgtg gacgtggaat acggcttccc
     5401 gcgaaacggc caggccattg acatcgccgt cctcaccgaa gcccgcgccc accggctccg
     5461 catctacgaa atctcccccg ccggccagct caccgacatc tccagccccc acaccaccgt
     5521 cttcgacggc caggcgcaac ccatgggcat cgcgctctac cgccgccccg ccgacggcgt
     5581 catctacgcc ctcgtcagca aaaaaaccga cccggaccac cgcatcctcg gccaatacca
     5641 gctcctgggc gaccccgacg gcaaagtccg tctcaaggaa atccggcgct tcggccgctt
     5701 cagcggcgcc ggcaccgtgg aagcggtggc ggtggacgac gaactgggct gggtctatta
     5761 cgccgacgaa tccaccggca tccgcaaata ccccgccgac cccgatcacc ccgcggccag
     5821 ccgcgaactc gccttgttcg gaaccgatga atactccgcc gaccgcgaag gcatcgccct
     5881 ctacccccag ccccgcggcc gcggctgcct catctgcacc gatcaactca aaaactcccg
     5941 ctacctcatc tacccccgcg ccggcccccc ggaaaacccg caccagcacg gccgggccat
     6001 caaaatcatc cgcggcggcg ccgacaacac cgacggcatc gaagtcgtct ccgaacccct
     6061 cggcccccgg ttcccccaag gcatcctcgt ggccatgaac agcaaaggcc gcaatttcaa
     6121 aatctacgac tggcgcgaca tcgcgcagac cggcaaaccc aaactctaaa cccgcccctc
     6181 ccaacctgcc gccccccggc ccccgcggaa aaaccccgcc gcccggcgcg ccgaaacggc
     6241 aacccccgcc cggagccgca aaccgccggt gaccccgccc gcctcttcgc tcggaaacgg
     6301 ataaccccgc cagcgatccc gccgctccgc cgcgtcagcg gaacgccacg act
//
LOCUS       DAOPQG010000154         5927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_14682_length_5927_cov_15.902929, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000154 DAOPQG010000000
VERSION     DAOPQG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14682_length_5927_cov_15.902929"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1358
                     /gene="hydA"
                     /locus_tag="PKI07_12975"
     CDS             6..1358
                     /gene="hydA"
                     /locus_tag="PKI07_12975"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOC51997.1"
                     /translation="MLEKVGHCKAKTVTPHYVADIWCAGETITRIAPNIQPPPGAEII
                     DAAAKYVFPGFVDPHVHIYLPCLGTLAKDSHATASQAALVGGTTTLIEMLGPDRSQDP
                     QEGFELWLDKAQGHCACDFTFHMTVSRYDGRVEKQLREIVRHGLSSFKVYLAYKDAFG
                     LSDRELYLTLRLARELGVITAAHCENAELVAELQSQQLAEGKTGPEWHHESRPPVIEA
                     EGVHHLLTFAKLLEAHVYIVHLSCAEALREVAVGKASGAKVWAETLIQYLLLDKSYAE
                     RPNFEGAKYVMSPPLRERRHQEILWGALRENEINTLASDHAPFDFATQKRLGVNDFTQ
                     IPNGLPAIEDRVNMLHTYGVKPGWLDLHRFVEVASTQAAKLFGLYPRKGAIRPGSDAD
                     LVIYAPTYRGTISAGRQVMNVDYNPFEGMGIEGRPQAVILRGQVVAREGRFIGQPGRG
                     "
     gene            1438..2103
                     /locus_tag="PKI07_12980"
     CDS             1438..2103
                     /locus_tag="PKI07_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC51998.1"
                     /translation="MQAPSPTIEIVQPKRFTAAAVMIISAYGVLLLLPLFFAILLVSL
                     LKFGLLTILIPLLVVAVTVSLLPFGLGNTYATRLVKSLPAEESRGEEAFIVQLTLSPR
                     IRSGIRAILDDADDLGCLRLASDALIFQGDSVRLVVPYDHIAEVQPRNIGLRGLFVYG
                     RRIKVSVSNWPEIDEMEFAERSSCNLPASKRITRRLYELLSAQVSSATTHVAARAPES
                     GHR"
     gene            2325..3680
                     /locus_tag="PKI07_12985"
     CDS             2325..3680
                     /locus_tag="PKI07_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC51999.1"
                     /translation="MKIDPAELGSSSARSDVNVQRHSRLTMPTTSPMEAETTLAPSPE
                     PAKRTLLFVDDEDPVRESLRVIFKDDYQIIMAGDGVTAIELVQKNKVDVAILDIKMAA
                     MSGLEVLERLKYVDPHIEVIMLTAFETTDLMRQALRLRASDYITKPFDVPTIRAAVAN
                     AMQRRALQGEIRSNADKLQQLLQEFQHQKVEEQIARTRGDIYASIIHDINGPLTVISG
                     FTQLLNQRLGNASQLELEDLGFIKDRLKVITRQVTNCVEISRRYLSFLRRQSAETPRV
                     GVNQLLVDLGHLVRVHPSLQNNQFILHPLAEDIAVGMNGTDFIQILLNLVVNAFQCSP
                     QTHTVEIEGCTLSEPIDFVTFKDGPQERLLNVENFEGTPPVLKISVRDNGPGIPPEAL
                     PKIFQAYYSTKGTQQGTGLGLNIVLRLIKEARGALHVHTQIGQGTCFTVFLPASPLAT
                     V"
     gene            3806..3879
                     /locus_tag="PKI07_12990"
     tRNA            3806..3879
                     /locus_tag="PKI07_12990"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3838..3840,aa:Gly,seq:tcc)
     gene            complement(4407..5249)
                     /locus_tag="PKI07_12995"
     CDS             complement(4407..5249)
                     /locus_tag="PKI07_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC52000.1"
                     /translation="MKETITITTLVENSVHARGLQAEHGLSFHLQAGSRSLLFDTGQS
                     DLFLRNARQLHINLAETEAIVLSHGHNDHSGGLRTARQTARRARLFLHRAALAPKFAR
                     NPDGSTRAIGMDASTAKEIESAPEEVVWTPKPVEIMEGIFVSGEIPRQTPFEDTGGPF
                     YLDSAATQPDTLPDDQALFFDTANGLVVLLGCAHSGVINTLEYIRRLTNGRPIQALLG
                     GMHLAAASHERMENTLAAFRRLGIRFLGPSHCTGFPAQAQLWNAFPGHCTTCAVGTTM
                     RFLR"
     gene            5456..5659
                     /locus_tag="PKI07_13000"
     CDS             5456..5659
                     /locus_tag="PKI07_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52001.1"
                     /translation="MRLVDLGDVVGRRKIAEIARLATILTQRQRHRLRLLRKKGAPSF
                     YKVPDYVVSYQALTRMNPETFAA"
BASE COUNT         1296 a         1612 c         1714 g         1305 t
ORIGIN      
        1 atcaaatcct agaaaaagtg ggtcactgca aagctaagac tgttacacca cactacgttg
       61 cggacatttg gtgcgcgggc gagacaatta cacgcatcgc gccgaatatc cagccgcctc
      121 cgggggcgga aattattgac gcagcggcta agtatgtgtt tccgggcttt gttgatccgc
      181 atgtgcatat ctatctgccg tgcctgggaa ccttggcgaa ggatagccac gcgaccgcca
      241 gccaggcggc gcttgttggc ggcacgacga ctctgataga aatgctgggg ccggatcggt
      301 cccaggatcc gcaggaaggg tttgaactgt ggctggataa agcgcaaggt cactgcgcct
      361 gcgactttac ttttcacatg acggtatccc gctatgacgg ccgggtggaa aaacaactgc
      421 gggaaatagt ccggcacggg cttagcagtt ttaaggtata tctcgcctac aaggacgcat
      481 tcgggctcag cgaccgggag ctatacctga cgttgcgatt ggcccgggaa ctgggggtca
      541 tcacggccgc ccactgtgaa aatgccgaac tggtcgcgga attgcagtcc caacagctgg
      601 ctgaaggcaa gactgggccg gagtggcacc acgaaagccg gccgccggtt attgaagcgg
      661 aaggagtgca tcatttgctg acatttgcaa agttacttga ggctcatgtt tacatcgttc
      721 atctgagctg cgcggaagct ttgcgcgagg ttgcggtggg gaaagcgagc ggagccaaag
      781 tctgggctga aacgctgatc caatatttac tattggataa gagctatgcc gagcgcccga
      841 attttgaagg ggcgaagtac gtaatgtcgc caccgctccg cgagcgccgt catcaggaaa
      901 tcctgtgggg cgccctgcgt gaaaacgaaa tcaacacgct ggcaagtgat catgccccgt
      961 tcgacttcgc cacgcagaaa cggttggggg tgaatgattt cacgcaaata cctaacggac
     1021 tccctgccat cgaagatcgg gtgaatatgc tccacactta tggggtgaag ccgggctggt
     1081 tggatttaca ccgtttcgtg gaggtggcga gcacgcaggc ggccaaactc ttcggccttt
     1141 acccgcgcaa aggcgcgatt cggcccggca gcgatgccga tctagtgatt tacgctccca
     1201 cctatcgcgg aacgatttca gccgggaggc aagttatgaa tgtggattat aatccatttg
     1261 aaggcatggg gattgaaggc cgtcctcagg ctgtgattct gcgaggccag gtcgtagccc
     1321 gcgaaggcag gtttatcggg cagccggggc ggggctgatt tcttcaacgc gaacccaatc
     1381 accattgatc ttgattttcc agcgcttccg cctccaataa aggtgaagta aagagtaatg
     1441 caagcccctt caccaactat cgaaatcgtt cagccgaagc ggtttaccgc ggcggcggtg
     1501 atgatcatta gcgcctacgg ggtgcttctg ctgctgccgc tgttctttgc tattctcctg
     1561 gttagcttgc ttaagttcgg cctgcttacc atcttgattc cgctgctggt ggtcgcggtt
     1621 acggttagcc tgcttccctt tggcctggga aacacctatg ccacccgcct ggttaagtcg
     1681 cttcctgccg aagagagcag gggagaggaa gcgtttattg tccagcttac cctgtcgcca
     1741 agaatcagat ccggcatccg ggccattttg gatgatgcag acgaccttgg ctgcctgcgt
     1801 cttgccagtg atgcgctgat ttttcaaggc gactcagtcc ggttagtcgt gccgtatgac
     1861 catattgccg aggttcaacc ccgcaacatt ggtttgcgag gtctctttgt ttatggccgg
     1921 cggatcaaag tttcggtctc gaactggccg gagatagatg aaatggaatt tgccgagcgt
     1981 tcgtcttgca acctgcccgc ttcgaaaagg attacccgcc gtttgtatga gttgctttcg
     2041 gcccaagtct cgtccgccac aacccacgtc gccgctcgcg ctccggaatc gggacatcgc
     2101 tgaacgccgc gccaccgcca aagccaaggt cggagcaaca tagccttctt gcgcggagcg
     2161 gctgaggcct cgctcgcgct ccgccgggct ataggtcagc ggtgagtgaa ttgctggaac
     2221 ttctgcttcc gatgtattcg gttttaattc gtgccagaca gccgctccgt ttcctctatt
     2281 cttttcgcct ctattctttt cgcttgtcga ggttttgcgt ttgggtgaag atagatcctg
     2341 ctgaacttgg tagttctagc gcgcggtctg atgttaatgt gcagcgacat tcacgtttga
     2401 cgatgcccac gacttcgcca atggaagccg agacgacact ggcgccgtcg ccagagcccg
     2461 cgaagcgcac cctccttttc gtggacgacg aggatcccgt gcgcgagtca ttgcgggtca
     2521 tctttaagga cgattaccag atcatcatgg ccggggacgg cgtgacggca atcgagttgg
     2581 tgcagaaaaa caaggtggat gtggctatcc tggatatcaa aatggccgca atgtccggcc
     2641 tcgaagtgtt ggagcgtctg aagtacgttg acccgcatat cgaggtgatc atgctgacgg
     2701 cttttgaaac caccgatctc atgcggcagg cgctgcgtct gcgggccagc gattatatca
     2761 ccaagccgtt tgatgttccg accattcgcg ccgcagtggc caacgccatg cagcgacgcg
     2821 ccttgcaggg cgaaatccgt tctaatgccg ataaactgca gcaactcctg caagagtttc
     2881 agcaccaaaa ggtcgaggag caaatcgcgc ggacgcgcgg cgacatttac gccagcatca
     2941 tccacgatat taacgggccg ctcacggtta tttccggatt cacccaactc ctgaaccagc
     3001 gccttggcaa tgcgtcacaa ttggaactgg aggacctcgg attcatcaaa gaccggctta
     3061 aagtcatcac ccgccaagtg acaaactgcg tcgaaatctc ccgccgctac ctcagttttc
     3121 tgcggcggca atccgcggaa acaccccggg tgggagtcaa tcaattgctt gtcgatcttg
     3181 gccatttggt ccgtgtgcat cccagcctcc aaaacaacca gttcatttta cacccgctgg
     3241 ccgaagacat cgccgtaggg atgaatggga ctgatttcat ccaaattctg ctcaatctgg
     3301 tggtcaatgc tttccagtgc tctccccaga cgcatacggt ggaaatcgaa ggctgcacgc
     3361 tgagcgaacc gattgatttt gtgacgttca aggatggccc ccaggagcgg ctgctcaatg
     3421 tggagaactt tgagggcact ccgccggttt tgaaaatttc cgtccgcgac aacgggccgg
     3481 gaattccgcc tgaggccttg cccaaaattt tccaagccta ttattcaacc aaaggcacgc
     3541 aacaaggcac cggcctgggg ttgaacatcg tattgcgcct tattaaggag gctagaggcg
     3601 ccctgcatgt tcacactcag atcggacagg gaacctgctt caccgtgttc ttgccggcct
     3661 ctcctctggc caccgtttaa accgccgcca acgcttaacc gcgttattct tcgctacggg
     3721 cagggtcacc ctattaaggg ccacagcaca aaacgtcctc cgtattgaca tcccgctttc
     3781 cgctaatatg attagctgca attgggcggg tgtagctcaa tggtagagct ccagccttcc
     3841 aagctggcta cgtgggttcg attcccatca cccgctccaa tgaatctcca aggcctctca
     3901 agtggcggcc tgcttaggcc cgggaagctt gttccattcg gcggagtttt gtccctgcgg
     3961 atttggattg agaacggctc atttccgggt gtttatagga aaaaaggagg taaaagattc
     4021 gactccagcg gcctgcgctg ggcaggggag aactggcctg ttaacggggg cggattgctg
     4081 acttggcatg gatgccggtt gggaacggga gaacgactct ggaagcgcgc taaatcttcg
     4141 ataagctaca acgctgaagc gtccaagccg ccgcaagaag ccaagcaact gctgaggccg
     4201 atttataggc agatttgcga ggcaggcaaa gcgcctgcta aggctgctgg caggaactcc
     4261 acgccgagga tcgagaggcc agcatgcgct atcggcgcaa caaaaagaac tccagcagcc
     4321 attgtttatt aagcaagaga acagtaatgc ctcacggagt gggctgtctc tcaaaagcgg
     4381 gctggattgc ttggctgcga gcggtgttat ctcaggaaac gcatagttgt cccaacggcg
     4441 cacgtagtac aatgcccggg aaaggcattc cataattggg cctgggcggg gaagcccgtg
     4501 cagtgagacg gccccaggaa gcggattccc agccgacgga aagcggcaag ggtgttttcc
     4561 atgcgttcgt ggctggccgc agccaggtgc atgccgccga gcaaagcctg gatgggtctg
     4621 ccgttggtga ggcggcgaat atattcaagg gtgttgatca cgcctgagtg cgcgcatccc
     4681 aggagcacca ctagaccgtt ggcggtatca aagaaaagcg cctgatcatc cggcaaagtg
     4741 tcgggctggg tggccgcaga atccagatag aaggggccgc cggtatcttc aaaaggggtt
     4801 tggcggggaa tctcaccgct gacgaagatg ccttccatga tttccacagg ttttggggtc
     4861 caaacgactt cttcaggtgc agattcgatc tctttggcgg ttgaagcatc catgccgatt
     4921 gcgcgggtag aaccgtccgg gttgcgggcg aacttcggcg caagggcggc gcggtgaaga
     4981 aatagccgcg cccggcgcgc cgtttgtcgg gcggtgcgca atcccccgga atggtcgtta
     5041 tggccgtgac ttagaacgat ggcttccgtt tctgccaaat tgatatggag ttgccgggcg
     5101 ttgcgcagga acaagtcgct ttggccagta tcaaagagga ggctgcgtga tccggcctgg
     5161 aggtggaaag aaagaccgtg ttcagcctgc aggccgcggg cgtggacgct attctcgacg
     5221 agggtggtaa tggtaatcgt ctctttcatg gtttcgctgc ggtggtcaaa gcaatcgcgt
     5281 taaggatatc gggtcaggca ggttccaaca agtatatgtg atcaaacgga aggtgaatgg
     5341 cccgggccct ttgatatcgg cgcttacgtt tttgtagata aagaggaaaa gattctgcta
     5401 agcccaaagc cgcttttcgg gttcaggggc tcgggtgaaa gcgtctatca atccaatgcg
     5461 gttggttgac cttggggacg tggttggccg gcgcaagatt gccgaaatcg cccgccttgc
     5521 cactatcttg acgcaacgcc aacgccatcg tttgagattg ctgcgaaaaa agggtgctcc
     5581 aagcttttat aaggtgccgg actatgtcgt gtcttatcaa gcgctcacgc gcatgaaccc
     5641 cgaaaccttt gccgcatagc aagacacccc ctctggaacg ctgaaccgcc acccaacccg
     5701 cagccgcaac ctcctcggct caagatctac acaaccaaag tccctgagca ggttcccgaa
     5761 ccaatcccgt caatcattcg ccctgtgcgc atagcccgag gctaaatctc caaccaacct
     5821 cactccaatt gggcgaatac gccgcccgcg atgagcgcaa cgcaagcagt cgccttgcgg
     5881 caaccttcgc ccgggagggg aaatgtcctc gccagtcttg gccccat
//
LOCUS       DAOPQG010000155         5717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_15424_length_5717_cov_15.196397, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000155 DAOPQG010000000
VERSION     DAOPQG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15424_length_5717_cov_15.196397"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(359..1459)
                     /gene="ilvC"
                     /locus_tag="PKI07_13005"
     CDS             complement(359..1459)
                     /gene="ilvC"
                     /locus_tag="PKI07_13005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOC52002.1"
                     /translation="MAIINFGGTKEQVITRKEFPMAKARKVLKNETIAIIGYGVQGPA
                     QSLNLRDNGFKVIIGQASAFKRDWDRAVADGWVPGKTLFEIDEAVRRGTVIQMLVSDA
                     AQRAIWPVIKRNLKPGDALYFSHGFSIAYQKQTGVIPPKNVDVIMVAPKGSGTSVRRN
                     FLTGAGINSSFAVEQDATGRAQERCLALGIGIGSGYLFPTTFKHEVFSDLTGERGVLM
                     GALAGIMEAQYDVLRAHKHTPSEAFNETVEELTQSLIRLVDENGMDWMYSNCSATAQR
                     GALDWKPKFKKAVLPVFKELYARVKSGQECARVLSACGKPNYQKQLGKELAAIGNSEM
                     WQAGKAVRALRPKEPARMIAAGTKGIGGRAAN"
     gene            complement(1615..2088)
                     /gene="ilvN"
                     /locus_tag="PKI07_13010"
     CDS             complement(1615..2088)
                     /gene="ilvN"
                     /locus_tag="PKI07_13010"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOC52003.1"
                     /translation="MRHTISVLVENKFGVLTRVAGLFSGRGYNIDSLNVAPTQDATVS
                     RMTIVTRGDDATVDQIVKQLKKLTNVINVQDFREGEYVDRELALVKVGVDSKTRSEVM
                     QITNIFRAKIVDIQPQNLTIEVTGDEDKVEKLLSLLQSFKIMEVTRTGKVALPRN"
     gene            complement(2214..2804)
                     /gene="recO"
                     /locus_tag="PKI07_13015"
     CDS             complement(2214..2804)
                     /gene="recO"
                     /locus_tag="PKI07_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOC52004.1"
                     /translation="MIQTATGLVFRTWPLTETSLIVHWLTPELGRLATVARGARRLQS
                     PFHGKLDLFYWADFSLSRSRRSELHTLREVRLRDTHGNLRRQLILLRQASRCAALIER
                     ATETETPLPGIFELMLSLLRHLSGPAAQSHTVLAFEFKLRLELGLLPGLAGSGLSPGA
                     AQLAQAFAKHDWPRIARLRPSPGQVAELEQFLRLKS"
     gene            complement(2801..3508)
                     /locus_tag="PKI07_13020"
     CDS             complement(2801..3508)
                     /locus_tag="PKI07_13020"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOC52005.1"
                     /translation="MKQSIFARWQANFWAGLVIVLPAVISLAVLRWLFGTVANITDLL
                     LVFLPARLTHQDGGSGPMYWYWSVVALLFAALLIGIVGLLARYYFGRRVIEWVDAALM
                     RVPLLNRIYSATKQVNDAFSSANKTAFRTVVLVRFPHGGMWSIGFITSEQQPEVEAKT
                     GEKVVCVFVPATPNPTSGFLLMVPETQVIKLDLTVANAIKYIISLGAIQPEGAPLPGA
                     AEAGPALPESGAGAGVP"
     gene            complement(3599..>5717)
                     /locus_tag="PKI07_13025"
     CDS             complement(3599..>5717)
                     /locus_tag="PKI07_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOC52006.1"
                     /translation="DCSCHASQYNATTNRKWYEITPASMRLDWTEAAIGNFKEYRIYR
                     SETPNVSESSTLLAVITNQAATTFVDGGLMARKTYYYRVYLHNHYDDSVGSNQSSATT
                     AGVELPWVADFETPQPGWTFTGTWTLWPGAGRNGSVALADSPGDYPNYSSHYAQFAAD
                     LRGSVWPVLRFWDRHFITAGDWGRVFISGDGGASWTRVYGVSATRTNWAEQSVDLSPW
                     KNSSQVWVRFQMNTGGNPPSDGWYLDDISLGEHSPAPAAYPFHETFEAGLDHWLHPVW
                     TVDTNQPFAGQYAVRDTVFPWMPPDTQLALVLGRELNLSNAVNPILTFRARGQLWYRT
                     RFRAQVSTDGGLVWNDLVALNNDWNQPWTRFQASLANYLNQTIRLRFIVWSEYGSAPD
                     QDLFLDDIGIGEPAPGAPILAAPANLASVPVVRPILSVTNAVDYQGDSLSYRFEVYAD
                     AALSNLVAQVPLVSAGTAITSWTVDTDLPDNAQYWWRCQATDGTNTGPWMATATFFVN
                     EINHAPAAPEIAGPPSGVLVTNLEARLFWFPSAGDPDEGDRVATYHIQVAADLAFTAP
                     VINATGIVAVAVPPGGNWVLSLPLQELPGAQNLVLGTRYYWRVSAQDLRGLSSDWSAG
                     EAAFQFGKVIPAPATLTGLRRGPNGTMSLEWEGAAGELYVEFSLSLTTPNWLTIAGPL
                     QGTNWTFTPVPGSPSGFFRVRSQ"
BASE COUNT          986 a         1796 c         1908 g         1027 t
ORIGIN      
        1 cacgcgcccg cctgggttgc cagcggtcac agggtctttt gttatcagcg cctgagtttc
       61 cctccccggc ttatgatcta caaaaccaag ggccctgtcc gttgcgatgg aattgctgct
      121 gcccggcgct ggagagcgtc atgctccaag ggtgcatccc aagggcgtcc gctgttgcgg
      181 ttcagcaagt caaggagttc caggcggcaa aacaacacgc ccgccaggca aactcagcgg
      241 gcgcgggctg tgtcctcttc ctgacaaaac ccgcgcggct ggggatccgg aaagctgccg
      301 ggcggcaaac gcaatgcccc ccggctcgaa gccggccgaa ccaggccttt gtaatgcgct
      361 aattggccgc ccggccgcca atgcctttgg tgccggcggc aatcatgcgc gcgggttctt
      421 tgggacggag cgcgcggacg gctttgccgg cctgccacat ctccgaattg ccaatcgcgg
      481 cgagttcctt gcccaactgc ttctggtaat tgggtttgcc gcatgccgaa agcacgcgcg
      541 cgcattcctg gccgctcttg acgcgcgcat acagctcttt aaagaccggc agcaccgctt
      601 tcttgaactt gggtttccaa tccagcgcgc cacgctgggc ggtcgccgag caattcgaat
      661 acatccagtc catgccgttc tcgtccacca gccggatgag gctttgcgtc agctcttcca
      721 ccgtctcgtt gaaggcctcg ctgggggtgt gtttgtgcgc gcgcaggaca tcgtattggg
      781 cttccatgat gcccgccagc gcccccatca gcactccgcg ctcgccggtc aggtcgctga
      841 acacttcgtg cttgaaggtc gtggggaaca aataacccga accgatgcca atcccgagcg
      901 ccaggcagcg ttcctgggcg cggccggtgg cgtcctgttc caccgcaaag ctcgagttga
      961 tgccggcgcc ggtaaggaaa ttgcggcgca ccgaggtgcc ggagcccttg ggggcgacca
     1021 tgatcacatc cacgttcttg ggcgggatga cgccggtctg cttctggtag gcgatggaga
     1081 atccatgcga gaaatacagc gcgtcgccgg gtttgaggtt ccgcttgatg acgggccaga
     1141 tggcgcgctg ggcggcatcg gacacgagca tctggatgac cgtgccgcgg cggacggctt
     1201 catcaatctc gaagagcgtt ttgcccggca cccagccgtc ggccacggcg cggtcccagt
     1261 cgcgcttgaa ggcgctggcc tggccgatga tgaccttgaa gccgttgtcg cgcaggttga
     1321 gcgattgcgc cggcccctgc acgccgtagc cgatgatggc gatggtctca ttcttgagca
     1381 ccttgcgcgc tttggccatg ggaaactctt tccgcgtgat gacctgttct ttggttccgc
     1441 cgaagttgat gattgccata attgtcctga tcttttgtat ttgcgtttgt gttatactaa
     1501 ccgcccgggc gcgcgggtca aggagcttgc cgggttgccg ccccgcccgg ggcgggccgc
     1561 gggccggccg gttcgacccc gccaacccgg ggcgcccggc gtatccgcgc cgggttaatt
     1621 ccgcggcagg gcaaccttgc cggtgcgggt cacttccatg atcttgaagg actgcaagag
     1681 gctgagcagc ttttccacct tgtcttcatc gcccgtcacc tcgatggtca ggttctgggg
     1741 ttgaatatcc acaattttgg cgcggaagat gttggtgatt tgcatcactt cggagcgggt
     1801 ctttgagtcc acgcccacct tgaccagcgc cagctcccgg tccacgtatt cgccttcgcg
     1861 gaagtcctgc acgttgataa cattggtcag ctttttaagc tgtttgacga tttggtccac
     1921 cgtggcatcg tcgccgcgcg tcacgatggt catgcgcgag acggttgcgt cctgcgtcgg
     1981 ggcgacgttg agcgagtcaa tattgtagcc ccggccgctg aacaggccgg ccacgcgggt
     2041 cagcacgccg aatttgtttt ccaccagaac tgaaatagtg tgtcgcataa atccccgctt
     2101 aagcgggcaa ggaaagctac aacccgcggc cgggcggagc aactggaaaa acccggaaac
     2161 cccggggcgc gcggcgggct gcggcgtccg cgggcgcgcg gccgtttggc ggcttatgat
     2221 ttcaggcgga ggaactgttc caactccgcg acctgcccgg ggctggggcg cagccgcgcg
     2281 atgcggggcc agtcgtgttt tgcaaacgcc tgggccagtt gggcggcgcc agggctcagc
     2341 ccgctcccgg ccaggccggg cagcaggccc agctccagcc gcagtttaaa ttcaaacgcc
     2401 agcacggtgt gggattgggc cgccgggccg gacaggtggc gcaggaggga cagcatcagc
     2461 tcgaagattc cgggcagcgg tgtttcggtt tcggtggcgc gctcgatcag cgccgcgcag
     2521 cgggaggcct gccgcaggag gatgagctgg cggcgcaggt tgccatgcgt atcgcgcagc
     2581 cgcacctcgc gcaaggtatg cagctcggag cgccggctgc ggctcaggct gaagtccgcc
     2641 cagtagaaca ggtccagctt gccgtggaag ggagattgga ggcggcgcgc gccccgggcc
     2701 acggtggcca ggcgccccag ttcgggcgtg agccagtgca cgatcaggct ggtttccgtg
     2761 agcggccagg tgcggaaaac gaggcccgta gccgtttgaa tcatggcacg cccgccccgg
     2821 cgcccgattc cggcagcgcc gggcccgcct ccgcggcgcc gggcagcggc gcgccctcgg
     2881 gctggatcgc gcccaggctg ataatgtact tgatggcgtt ggccacggtc aggtcgagtt
     2941 taatcacctg ggtctccggc accatcagca aaaaccccga ggtggggttg ggcgtggccg
     3001 ggacgaatac gcacaccacc ttttcgccgg ttttcgcctc cacctccggc tgctgttcgc
     3061 tggtaatgaa gccgatggac cacatgccgc catgcgggaa ccggaccaga acgacggtgc
     3121 gaaaggcggt cttgttggcg gaggaaaagg cgtcgttgac ctgcttggtg gcgctgtaaa
     3181 tccggttgag gaggggaacc cgcatgaggg ccgcatccac ccattcgatc acccgccggc
     3241 caaagtagta gcgggccagc agcccgacaa tgccgatcag cagggcggca aacaacaggg
     3301 ccaccacgct ccagtaccaa tacatggggc cgctcccgcc gtcctggtgg gtcagccggg
     3361 ccggcagaaa gaccagcagc aaatccgtga tgttggcaac cgtgccgaaa agccaccgca
     3421 gcaccgccag cgagatgacg gccggcagca ctatgaccag gcccgcccag aagttggcct
     3481 gccagcgcgc gaaaatactt tgcttcaccg cgccagttta gccgcgccgg aggccgggct
     3541 caagaccccg cacccgcccg cccgccgccg ccgcggttgc gcgcccgcgg cgggcggctt
     3601 attggctgcg cacccggaag aagcccgagg gactgccggg caccggcgtg aaggtccagt
     3661 tggtgccctg gagcgggccg gcgatggtca gccagttggg cgtggtcagc gagaggctga
     3721 actcgacata cagctcgccc gccgcccctt cccattccag ggacatggtg ccattcggcc
     3781 cccggcgcaa cccggtgagc gtagccgggg ccgggatcac cttgccgaac tggaaggccg
     3841 cttcgccggc cgaccagtcg gagcttagcc cgcgcagatc ctgcgcgctc acgcgccagt
     3901 agtagcgcgt tccgaggacc agattctgcg cgccgggcag ttcctggagt gggagggaca
     3961 atacccagtt gccgcccggc ggcacggcga cggccacgat gccggtggcg ttgatcaccg
     4021 gggccgtgaa ggccaggtcc gcggccacct ggatatggta ggtagccacg cggtcgccct
     4081 catcgggatc gccggccgag gggaaccaga agagccgggc ctcgaggttc gtcaccagga
     4141 ccccggatgg cggcccggca atttccggcg cggcgggggc gtggttgatt tcattcacga
     4201 aaaaggtcgc cgtcgccatc caagggcccg tgttggtgcc atccgtcgcc tggcaacgcc
     4261 accagtactg cgcgttgtca ggcaggtcgg tgtccacggt ccagcttgtg atggctgttc
     4321 cggcggatac cagcggcacc tgggccacca ggttcgagag cgccgcatcc gcatagacct
     4381 cgaagcggta ggagagcgag tcgccctggt aatccaccgc gttggtcacc gataagatcg
     4441 ggcgcacgac gggcacgctg gccaggtttg ccggggccgc aaggatgggc gctcccggcg
     4501 cgggttcgcc aatgccaata tcgtccagga acaggtcttg atccggcgcg ctgccatact
     4561 cgctccatac gataaaccgc aggcggatgg tttggttgag ataattcgca agcgacgcct
     4621 ggaagcgcgt ccagggctga ttccagtcgt tgttcagcgc gaccaggtcg ttccagacca
     4681 ggccgccgtc ggtcgagacc tgcgcgcgaa agcgggttcg ataccagagc tggccgcgcg
     4741 ccctgaaggt caggatggga ttgaccgcgt tgctcaggtt gagttcccgt cccaatacca
     4801 gcgccagttg cgtgtcgggc ggcatccagg gaaagaccgt gtcgcggaca gcgtattgcc
     4861 cggcgaaggg ttggttggtg tccaccgtcc agaccgggtg cagccagtgg tccagccccg
     4921 cctcgaaggt ttcatggaac ggataagccg ccggggcggg agagtgttcg cccagcgaga
     4981 tatcgtccag gtaccaacca tcgcttgggg ggttgccgcc ggtgttcatc tggaagcgca
     5041 cccacacctg gctgctgttt ttccaggggg agaggtcaac cgactgctcg gcccagttgg
     5101 tgcgcgttgc cgagactcca tagacccggg tccagctcgc tccgccatca ccggagatga
     5161 atacccggcc ccagtcgccg gccgtgatga agtgccggtc ccagaagcgc aagaccggcc
     5221 agacggagcc gcgcaagtcg gcggcgaact gtgcgtaatg cgaagagtag ttggggtagt
     5281 cgccgggtga atccgccagg gcaacggaac cgttgcgtcc cgcgcccggc caaagcgtcc
     5341 aggtgccggt gaacgtccag cccggctgcg gggtctcgaa atcggccacc cacggcagtt
     5401 ccacgccggc cgtcgttgcg gaggactggt tggagccgac gctatcgtcg tagtggttgt
     5461 ggagatacac gcggtaatag taggtcttgc gcgccatcag gccgccgtcc acaaaggtgg
     5521 tcgccgcctg gttcgtgatg acggccagca acgtcgaact ttcgctgacg ttgggtgtct
     5581 ccgaccggta aatgcggtat tctttgaagt tgccaatggc cgcctcggtc caatccaggc
     5641 gcatggaagc cggcgtgatc tcataccatt tcctgttagt agttgcattg tattgagagg
     5701 catggcaaga acagtca
//
LOCUS       DAOPQG010000156         5671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_15593_length_5671_cov_19.023326, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000156 DAOPQG010000000
VERSION     DAOPQG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15593_length_5671_cov_19.023326"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKI07_13030"
     CDS             complement(<1..544)
                     /locus_tag="PKI07_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52007.1"
                     /translation="MKILSAPRTGSLGAETYYQSPFGVCARRRTVPRDRPSNRKAAAR
                     SYFGISSREWGLKLSEAQRERWNAAALHVPSRPWMGQYSHLSGQQFCVQINSTLRGLG
                     LAPVEEPPAPVVFGPNPVTGLEIVNDAEDGVRLRLNVGAVHEDIMVFGQPPCSAGRMK
                     PRRAYYLGLLGPSQNGQSDIT"
     gene            complement(737..1342)
                     /locus_tag="PKI07_13035"
     CDS             complement(737..1342)
                     /locus_tag="PKI07_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52008.1"
                     /translation="MAATVPTEALTRYLAATPQQQEVIDRFLRRGAPASGAGPRGGAR
                     DEVREALRRIEQKVDAVKARLEGGAAAPEAPASAGEAERVFLLLQRLEGEPKQRKAPL
                     PLVFRLLVLRGLSQRAAAARCGCAESLISARAAALERAFGLSLERLRAYASALVEMET
                     AVKGGRTRRKRDGQPDDFAHGDEAAEGGEAEEDEGGCPDAA"
     gene            complement(1361..1618)
                     /locus_tag="PKI07_13040"
     CDS             complement(1361..1618)
                     /locus_tag="PKI07_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52009.1"
                     /translation="MNSSRPKPMGTPVNASQEAAAAPEWWTALPAGERQFLRRLARLV
                     RAARRSRAPEVARAAAKWERALACFIKVRLRHERRGGGRAH"
     gene            1850..2914
                     /locus_tag="PKI07_13045"
     CDS             1850..2914
                     /locus_tag="PKI07_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOC52010.1"
                     /translation="MKAEPRSSSPARAPRAPRRRPVKPGPVKRRALGRMKKILGLLQD
                     GKYPNCATVAAELEISARTVARDLDCMRDDLELPIEYDKQRHGYYLTAPVAGLPIVPV
                     SSRELFAVCVAHKALEHYRGTALEKPLELAFKRFADRLDDDERFTLQSLDEVLSLRPF
                     APEDADLKLFEQVTGALTDRLALRFHYRKPGQRHAEPRRVHPYHLFEFSHRWYLLAHD
                     LQRRALRTFVLGRMRDVVVTRDKFQIPPGFNPRQHFAASLGIMSGQGDYHVVIEMDPW
                     LTDILRGRRWHPSQQIKEFPDGRSQLRLRLSGLEEIEQYILSWGAHATVLAPPELRQR
                     LLSTTRELARRYTHPAPAPA"
     gene            complement(2948..4960)
                     /locus_tag="PKI07_13050"
     CDS             complement(2948..4960)
                     /locus_tag="PKI07_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HOC52011.1"
                     /translation="MKDASQPGCPAAGLSRAWYEAPVAEFRRHEPDLIIGRLTSNSDF
                     TVLPNQREAWRVQIDLLKNCLDGLNGWLFLEFNIPRMGRRIDAVLLLGPIIFVIEFKV
                     GERSFAQAAVEQVWDYALDLKNFHEASHAAAIVPILLATEAAGTERPQLQADEDKVYR
                     PLRIDAGRLREVVDLARQEIEGEQINEADWLQAPYRPTPTIIEAARALYAQHSVEAIA
                     RYDAGAQNLRVTSARLEELVDEAKSRGRKLVCFVTGVPGAGKTLVGLNLATQRRDPNQ
                     PTHAVFLSGNRALVAVLREALTRDEVARQRLQGTRVRKGKVAESVKAFIQNVHHFRDD
                     ALFDPRPPSEHVAIFDEAQRAWNQQQTAHFMRQKKKQAGFEQSEPEFLISCMDRHPDW
                     AAVICLVGGGQEIHRGEAGLDAWLKAVNARFPHWHLHLSARLTDSEYAAGKALEVIAG
                     RENSHFDDSLHLAVSMRSFRAETVSAFVKALLDCEKQVARAALAQFAKRYPVVVTRNL
                     DKARQWIRARARGSERYGLVASSKAQRLKPHAVDIRVDVDPVHWFLNSKADPRSSYYL
                     EDAATEFQVQGLELDWACVTWDGDLRFTGSDWGYYDFRGTRWCKVQNDFNRRYLRNAY
                     RVLLTRARQGMIIFVPPGDGNDPTRAPGFYDPTFNYLTEVGIPVVE"
     gene            complement(5029..>5671)
                     /locus_tag="PKI07_13055"
     CDS             complement(5029..>5671)
                     /locus_tag="PKI07_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HOC52012.1"
                     /translation="PYLFKAKNIQTAGDLIKNLLDAHLSSQEETILGDFLEGLAIFIC
                     SHVFGGRKSAAPGIDLEFERDNIYYIVAIKSGPHWGNSRAIAKMREDFRKAKQTLRTS
                     RSKLNVLSINGCCYGRESAEDKGDYLKLCGQRFWSHVTGIEDLYLRIVKPIGHKAQER
                     NDEFAAEYAKVINCFTAQFLGGFCDTKGAIQWDNLVRFNSGADSVEFPRTRAT"
BASE COUNT         1001 a         2094 c         1543 g         1033 t
ORIGIN      
        1 ccgtgatgtc gctttgcccg ttctggcttg ggcccaacag gcccaggtaa taggcacggc
       61 ggggtttcat ccggcccgca ctgcagggtg gctgcccaaa gaccatgatg tcttcatgga
      121 cggctccgac gttgagtcgc agccgaaccc cgtcttccgc gtcattgacg atctccaacc
      181 cggtgacggg attggggccg aagaccaccg gggcgggcgg ttcctccacc ggcgccagac
      241 ccagcccccg cagggtgctg ttgatctgga cgcaaaactg ttgcccactc aggtgcgaat
      301 actgccccat ccagggccgg ctgggaacgt gcagcgccgc ggcgttccaa cgctcgcgct
      361 gcgcttcgct gagcttgagt ccccactccc gcgaggagat cccgaagtac gaccgtgcgg
      421 ccgctttgcg gttagaaggc cggtcccggg gaacggtgcg ccgccgggcg cacacgccga
      481 acggggactg gtagtaagtt tccgcgccca ggctgcccgt acgcggagca gatagtattt
      541 tcataacgtc tgttctctct aattgttgtt ttaattgtct gcctattacg gcccgaaggc
      601 gattcggctt cccgccactc cttgggcagg aagtccgccc cgaggtaata cgggaaggct
      661 ggcgggcggg gaggcttcgc gcaagggcaa ttttttgggg ttggcggtgg caggccggtg
      721 cggaggccgc gtgctctcac gcggcgtctg gacatcctcc ctcatcctcc tcagcttcgc
      781 cgccttccgc cgcttcgtcc ccgtgcgcaa aatcatctgg ctgtccgtcg cgcttgcgcc
      841 gcgtccggcc gcccttgacc gcggtttcca tttccaccag cgcggacgca tacgctcgca
      901 gccgttcgag gctcaaccca aacgcccgct ccagcgccgc cgcccgcgcc gaaatcaacg
      961 actccgcgca cccgcatcgc gccgccgccg cccgctgcga caatccccgc aacaccagca
     1021 accggaacac cagcggcagc ggcgcctttc gctgcttcgg ctcgccctcc agccgttgca
     1081 gcaggagaaa cacccgctcc gcctcgcccg cgctcgccgg ggcctccggc gccgccgcgc
     1141 cgccctccag ccgcgctttc accgcgtcca ccttttgctc gatccgccgc agcgcctccc
     1201 gcacctcatc ccgcgccccg ccccgcggcc cggcgccgga cgcgggcgcg ccccgccgca
     1261 ggaagcggtc aatgacctcc tgctgctgcg gcgtcgccgc gagataccgc gtgagcgcct
     1321 ccgttggaac cgtcgctgcc atcccggcaa tccgcgggct tcaatgcgcg cggcctcccc
     1381 cccgccgctc gtgccgcagc cgcaccttga taaagcacgc cagcgcccgc tcccatttcg
     1441 ccgccgcccg ggccacctcc ggcgcgcggc tccgccgggc ggcgcgcacc agccgcgcca
     1501 gccgccgcag aaactgccgc tcccccgccg gcagcgcggt ccaccactcc ggcgccgccg
     1561 ccgcttcctg ggacgcattc accggcgtcc ccatcggttt tggtctggaa ctgttcattt
     1621 gtccagcact ctacaaccgc ccctctgaca aatacggggg taggccggca aatgtttttc
     1681 ccaaagttat tcacattttg ccatcccacc ggacggctcc cggcggggcc ggcgccggcc
     1741 ccgctcccgg cgcgctcccc cccgaccccc cgcgccccgt ccgccggccc gcaaattatt
     1801 tccggtctcc ccctccaaat gtcagacccc ctctgctatg ctgcccgcca tgaaagctga
     1861 accgcgttcg tcgtccccgg cccgcgctcc ccgggccccc cgccgccgcc ccgtcaagcc
     1921 cggccccgtg aagcgccgcg ccctgggccg catgaagaaa atcctcggcc tcctccagga
     1981 cggcaaatac cccaactgcg ccaccgtcgc cgccgaactt gaaatttccg cccgcaccgt
     2041 cgcgcgcgac ctggactgca tgcgcgacga cctggaactg cccatcgaat acgacaaaca
     2101 gcgccacggc tattacctga ccgcccccgt cgcgggcctg cccatcgtcc ccgtcagctc
     2161 ccgcgagctc ttcgccgtct gcgtcgccca caaagccctc gaacattacc gcggcaccgc
     2221 cctggaaaaa cccctcgaac tggccttcaa acgtttcgcc gaccgcctcg acgacgacga
     2281 acgcttcacc ctgcaaagcc ttgacgaagt cctctccctt cgcccctttg cgcccgaaga
     2341 cgccgacctc aaactcttcg agcaagtcac cggcgccctc accgaccgcc tggccctgcg
     2401 cttccactac cgcaaacccg gccagcgcca cgccgaaccg cgccgcgtcc acccctacca
     2461 cctgttcgaa ttcagccacc gctggtacct cctggcccac gacctccagc gccgcgccct
     2521 ccgcaccttc gtcctgggcc gcatgcgcga cgtcgtcgtc acccgcgaca aattccaaat
     2581 cccccccggc ttcaaccccc gccagcactt cgccgccagc ctcggcatca tgagcggcca
     2641 gggcgactac cacgtcgtca tcgaaatgga cccttggctc accgacatcc tgcgcggccg
     2701 ccgctggcat cccagccagc aaatcaagga attccccgat ggccgctccc agctccgcct
     2761 ccgcctgagc ggcctcgaag aaatcgagca atacatcctg agctggggcg cgcacgccac
     2821 cgtcctggcc ccgcccgagc tgcgccagcg cctcctttcc accacccgcg aactcgcccg
     2881 ccgctacacc caccccgccc ccgccccggc ctgacccccg cccgcgcgcc gccccttcgg
     2941 gtagtaatta ctccacaacc ggaatcccca cctcggttag gtaattgaac gtggggtcgt
     3001 agaatccggg cgctcgcgtc ggatcgttcc catccccggg cggaacaaaa ataatcattc
     3061 cctgccgcgc gcgggtcagc aggacccggt acgcattgcg gagatagcgg cgattgaaat
     3121 cgttttgaac cttgcaccaa cgggtgccac ggaaatcata ataaccccaa tctgagccag
     3181 tgaaccgcag atcgccatcc catgtgacac acgcccaatc aagctccaag ccctgcacct
     3241 gaaactcggt ggcggcatct tccaggtagt agctcgaccg cggatccgcc ttgctgttta
     3301 aaaaccaatg gactgggtca acatccacac gaatgtcaac ggcgtgcggt ttcagccgct
     3361 gggcttttga cgacgccacc aggccatacc gctcgcttcc ccgggcgcgc gcccggatcc
     3421 actgcctcgc tttgtccaga ttgcgggtga ccaccaccgg gtatcgtttc gcaaattgcg
     3481 ccagcgccgc acgcgcgact tgcttttcgc aatcgagcaa cgcctttaca aaagccgaca
     3541 cggtttccgc ccggaacgat cgcatggata cggccaggtg gaggctgtca tcgaaatggc
     3601 tgttctcccg tccagcgatt acttccaacg ccttgccggc tgcatattcg ctatccgtaa
     3661 gccgtgccga aaggtgcagg tgccaatgcg gaaacctggc attgaccgct ttcaaccacg
     3721 catcaaggcc agcctctccc cggtggattt cctggccgcc gccgaccagg cagatgaccg
     3781 cggcccaatc cggatggcgg tccatgcagg aaatcaggaa ctccggctcg gattgttcga
     3841 atcccgcttg tttcttcttc tggcgcatga aatgggccgt ctgctgctgg ttccacgcgc
     3901 gctgcgcttc gtcgaaaatg gcgacatgct cgctcggggg cctggggtca aacaacgcgt
     3961 cgtcacggaa atgatgaaca ttctgaatga acgccttgac gctttccgcc acctttccct
     4021 tccgaacccg ggttccctgg agccgttgtc gcgccacttc atctcgggta agcgcctccc
     4081 tcagcacggc gaccaaggcc cggtttcccg agagaaagac cgcgtgggtg ggttgattcg
     4141 ggtcccggcg ctgggtggca agattcagcc ccaccaaggt cttgccggcg cccggaaccc
     4201 cggtgacgaa gcaaaccagt tttcggcccc gggacttcgc ctcgtccacc aactcctcca
     4261 accgggccga agtgacccgc aggttttgcg cgcccgcgtc ataacgcgca atcgcctcga
     4321 ccgaatgttg ggcataaaga gcccgtgccg cttcgatgat cgttggcgtc ggacgatacg
     4381 gcgcctgcaa ccagtccgct tcattgatct gttcaccctc gatttcttgc cgggccaggt
     4441 ccacaacctc gcgcaggcgg ccggcgtcaa ttcgaagcgg gcgataaacc ttgtcttcat
     4501 ccgcctggag ttgtggtcgt tccgttcccg cggcttcggt cgccagaaga atcggaacga
     4561 tggccgccgc atgactggcc tcgtggaaat ttttcaaatc cagcgcataa tcccacacct
     4621 gctccaccgc ggcctgcgcg aatgaacgct cccccacttt gaactcgatc acaaagatga
     4681 tcggacccaa caggagcacg gcgtcaatcc tccggcccat gcgcgggatg ttgaactcca
     4741 aaaacagcca gccgttcaat ccgtccaggc agtttttcag caagtctatc tggacccgcc
     4801 acgcttccct ctgattgggg agcactgtaa aatcgctatt gctcgtcagt cgcccaatta
     4861 taaggtccgg ctcatggcgt cgaaactccg ccacgggcgc ttcgtaccag gcccgcgaca
     4921 accccgccgc cgggcagccg ggctgggacg cgtctttcat tggctgtgaa taaaaggttg
     4981 cctggggcgc gcccgcagcc gcgaagcgcc gcgccggtga gtcgttggct aggtggcccg
     5041 cgttcgtgga aattccacgg aatctgcgcc ggagttaaag cggaccaggt tgtcccactg
     5101 aatggctcct ttcgtatcac aaaacccgcc cagaaactga gccgtaaaac agttgatgac
     5161 tttggcgtat tccgcggcaa attcgtcgtt gcgctcctgg gctttgtgcc cgatgggctt
     5221 aacgatgcgc aggtaaagat cttctatccc ggtcacatga ctccagaagc gctgaccgca
     5281 gagttttaga taatcgcctt tgtcttcggc ggactcccgc ccatagcagc acccattgat
     5341 agataataca ttcagcttcg aacggctggt gcgcagggtt tgtttcgctt tgcggaaatc
     5401 ctcccgcatt ttcgcgatgg cacggctgtt gccccagtgg gggccggact taatcgccac
     5461 gatatagtag atgttgtccc gctcaaattc caggtcgatg cccggcgcgg ccgatttacg
     5521 cccgccgaag acatggctgc aaatgaaaat cgccaggccc tccagaaagt cacccagaat
     5581 tgtttcctct tgcgacgaga ggtgggcgtc cagaaggttc ttaatcaaat ccccggcggt
     5641 ttgaatattc ttggccttga aaagataagg g
//
LOCUS       DAOPQG010000157         5620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_15794_length_5620_cov_13.172507, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000157 DAOPQG010000000
VERSION     DAOPQG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15794_length_5620_cov_13.172507"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="PKI07_13060"
     CDS             <1..1706
                     /locus_tag="PKI07_13060"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOC52013.1"
                     /translation="RKLELATALESFNLFEPRRFGRGLMMKTAFALAFVLLAWPAAAA
                     EAQPGPSIYEAPPPPAPDNPIDQQVRERLSTLGIPPTLCSDAVFIRRVSLDLLGKLPT
                     AAEARAFITDPDTANKRRRLIDRWLQREEFADYWAMKWGDLLRIKAEFPVNLWPNAAQ
                     AYHRWVRAALARNQPYDQFARELLTASGSNFRVGPVNFYRALPNRTPEGIAAGAVLIF
                     MGTRTDAWPESQLAGLAACFSQVGYKPTREWKEEIVFWDPLKDIPEATNGLAVLPPPP
                     RSNAPPRTAVFPDGTRAPLSAERDPREVFADWLTAPGNPWFARNIANRVWAWLLGRGL
                     IHEPDDLRPDNPPSNPALLAYLEKELVASHYDLKHLFRIILNSQTYQSSSLPRGDVAL
                     AEANQGCYVLRRLEAEVLIDAINQITGTSDLYTSAIPEPFTYIPSDQPAVALADGSIT
                     SPFLALFGRSARATGMENERINKMLPPQWLHLLNSSHIQRKLEQGPGLRALGAPPRQP
                     RVIATNLYLAILSRFPSPDELKVLENYIRSARLKSEPRREHLVDIAWALINSPEFIYR
                     H"
     gene            1750..3156
                     /locus_tag="PKI07_13065"
     CDS             1750..3156
                     /locus_tag="PKI07_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOC52014.1"
                     /translation="MSRQPSPPRPPAPAAPLGLSLHLPLSRRQALRLSLFSTAGLLVS
                     PRHLFGAAAPAPAAASAAAGPAVRKAKSIIQIFLWGGMSHNDTWDPKPDAGYDYNGPM
                     KTFLPTNVPGITLSEWFPRLAKQADKFALIRSMTHRNNGHETAAYLMQTAHAPGGRLA
                     YPSVGAVFAYLRSEDYQGLIPPYVVLTQPQGRFSEEGFLGPRFKPFATGGDPNASRFE
                     VEGVVARGISDDRQKNRRDLLEQLNTLGRAAAANPLMAASEEAKQNAYGLILGEGRAV
                     FDLGREKPELRDLYGRHTFGQDCLAARRLVEAGVPYIVINYPGGWDTHKDHFQTMNRQ
                     CPQLDQGLAALLQDLHQRGLLDSTLVWCSGEFGRTPKIDWQPPWNGGRNHYGNVFTVL
                     VAGGGFKGGQVVGASTAKAEEVQDRPVYPADLLGSIYHLGGLDPNAKLPHPMGLDAFV
                     LPSAAEGVKSAGLLTEIM"
     gene            3176..5383
                     /locus_tag="PKI07_13070"
     CDS             3176..5383
                     /locus_tag="PKI07_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52015.1"
                     /translation="MPLSALSRAAALLAAALLAAALPARAQPRPYVGFVYPAGGQQGT
                     TFAVKLGGQNLNDAREARVTGTGVSARVVECYRRLNNQEMNLLNEQLRQLKRTTSASD
                     AAVMNADTPMMAAPLTPAPASASPPGAAPEAAPAAADRQLMARIEKRAAEYCNRPASA
                     ALACLVFLEVTIAPDAQPGARELRIRTPLGLSNPMVFHVGQLPEHTRPPMLTSSQQVL
                     GKEALSLRRRPPEEALVRVSLPCVLNGQVASGEINQYRFTARKGQRLVITTQARQLVP
                     FIADAVPGWFQPVLLLRDAAGREIAYQDDYRFNPDPTLLCQVPRDGDYILSIYDSLYR
                     GREDFVYRLSLGELPFITSIFPLGASTNAAPPIRAQGWNLDSHRLAPPPAGAGPGIHF
                     LTARKQDLLSPPVPFALDALPDRAEQEPNQPQERAQPVSLPIMINGHINQPGDEDLFR
                     FTGRAGQTIAAEVFARRLASPLDSILKLTDSAGRLLAWNDDCEDFAAGINTHHADSFL
                     LATLPADGDYCIHLGDIARHAGPEYAYRLCLRPARPDFDLRVVPSSVNLRPRSSAALS
                     VHVLRREGFTGPIKISLADPPPGFFANPVFLSGTQTVARLSLKTSLLETPEPVRLTLA
                     GTAKIGNRQVSRIAVPAEDRMQAFLWRHLVPASELLATVYNPAAPPPPGRPLPTLPPA
                     PLAGTTTNSSGDKPKFTRQQIARRLRELNSLFQENLLTEDFYLRKVAECEPAL"
BASE COUNT          915 a         2474 c         1432 g          799 t
ORIGIN      
        1 cgcgcaaact ggagttggca acggcgctgg aaagctttaa cttatttgag ccgcgccgat
       61 ttggccgcgg cctgatgatg aaaaccgcct tcgcgcttgc gtttgtgctg ctggcatggc
      121 ccgccgccgc cgccgaggcc cagcccggcc cgtccatcta cgaggccccg ccgcccccgg
      181 cgcccgacaa cccgattgac cagcaggtcc gggagcggtt gtccaccctg ggcatcccgc
      241 cgacgctctg ctccgacgcc gtgttcatcc ggcgcgtcag cctggacctc ctcggcaaac
      301 tgcccaccgc cgccgaagcc cgcgccttca tcaccgaccc cgacaccgca aacaaacgcc
      361 gccgcctcat tgaccgctgg ctccagcgcg aggagttcgc cgattactgg gcgatgaagt
      421 ggggcgacct cctccgcatc aaggccgagt tcccggtcaa cctctggccc aacgccgccc
      481 aggcctacca tcgctgggtc cgcgccgccc tcgcccgcaa ccagccctac gaccagttcg
      541 cccgcgaact gctcaccgcc agcggcagca acttccgcgt cggcccggtc aacttctacc
      601 gcgccctccc caaccgcacc ccggaaggca tcgccgccgg cgccgtcctc atcttcatgg
      661 gcacgcgcac cgacgcctgg ccggaatccc agctcgccgg gctggcggcc tgcttctccc
      721 aggtcggcta caagcccacg cgcgagtgga aggaggaaat cgtcttctgg gacccgctga
      781 aagacatccc cgaagccacc aacggcctcg ccgtcctccc gccgccgccc cgctccaacg
      841 ccccgccccg caccgccgtc tttcccgacg gcacgcgcgc cccgctctcc gccgagcgcg
      901 acccccgcga ggtctttgcc gactggctca ccgctcccgg caacccctgg ttcgcccgca
      961 atatcgccaa ccgcgtctgg gcctggctcc tgggccgcgg cctcatccac gagccggacg
     1021 acctccgccc ggacaacccg cccagcaacc ccgcgctcct ggcctatctc gaaaaggagc
     1081 tggtcgccag ccactacgac ctcaagcatc ttttccggat catcctcaac tcccaaacct
     1141 accagtcctc ctccctgccc cggggcgacg tcgcgctggc cgaagccaac cagggctgct
     1201 acgtcctgcg ccgcctcgag gccgaggtgc tgattgacgc catcaaccaa atcaccggca
     1261 cgtccgacct ctacaccagc gccatcccgg agccgttcac ttacatcccc tccgaccagc
     1321 ccgccgtggc cctggccgac ggcagcatca ccagcccgtt cctcgccctg ttcggccgct
     1381 ccgcccgcgc caccggcatg gaaaacgagc gcatcaacaa gatgctcccg ccgcagtggc
     1441 tccacctgct caactccagc cacatccagc gcaagctcga acaaggcccc ggcctccgcg
     1501 ccctgggcgc cccgccccgc cagccccggg tcatcgccac caacctctac ctcgccatcc
     1561 tctcccgctt cccctccccc gacgaactta aagtcctcga aaattatatc cgctccgccc
     1621 gcctcaaatc cgagccgcgg cgcgagcatt tggtggacat cgcctgggcg ttgatcaaca
     1681 gccccgaatt catctaccgc cattaagcat gctgaccctc ctctcccata cccattcctc
     1741 cgtcaaatta tgagccgcca accctctccc ccccgaccgc ccgcgcccgc cgcgcccctg
     1801 ggcctgtcgc tccacctgcc gctctcccgc cgccaggcct tgcgcctcag cctcttcagc
     1861 accgccggct tgctcgtgag cccccgccac ctgttcggcg ccgccgcgcc cgcgcccgcc
     1921 gccgcgtccg ccgccgccgg ccccgccgtc cgcaaagcca aatccatcat ccagattttc
     1981 ctctggggcg gcatgtccca caacgacacc tgggatccca agcccgacgc cggctacgac
     2041 tacaacgggc cgatgaaaac ctttctgccc accaacgtcc cgggcatcac cctcagcgaa
     2101 tggttcccgc ggctggccaa acaggccgac aagttcgccc tcatccgcag catgacccac
     2161 cgcaacaacg ggcatgaaac cgccgcctac ctcatgcaaa ccgcccacgc ccccggcggc
     2221 cgcctcgcct accccagcgt cggcgcggtc ttcgcctatc tccgcagcga agattaccag
     2281 ggcctgatcc cgccctacgt cgtgctcacc cagccccagg gccggttctc cgaagaggga
     2341 ttcctcggcc cgcgcttcaa gccgttcgcc accggcggcg atcccaacgc cagccgcttc
     2401 gaggtggaag gggtggtcgc ccgcggcatc agcgacgacc gccagaaaaa ccggcgcgac
     2461 ctgctcgagc aacttaacac cctgggccgg gccgccgccg ccaacccgct catggccgcg
     2521 tccgaggaag ccaaacaaaa cgcctacggc ctcatcctcg gcgaaggccg cgccgtgttc
     2581 gacctgggac gcgaaaaacc cgagctgcgc gacctctacg gccgccacac cttcggccag
     2641 gattgcctcg ccgcccgccg cctggtcgag gccggcgtcc cgtacatcgt catcaattac
     2701 cccggcggct gggacaccca caaagaccac ttccaaacca tgaaccggca atgcccccag
     2761 ctcgaccagg gcctggccgc gctgctccag gacctccacc agcgcggctt gctcgactcg
     2821 accctcgtct ggtgctccgg cgagttcggc cgcacgccca aaattgactg gcagccgccc
     2881 tggaacggcg gccgcaacca ctacggcaat gtcttcaccg tcctggtcgc cggcggcggc
     2941 ttcaaaggcg gccaggtcgt cggcgcgtcc accgccaaag ccgaggaagt ccaggaccgc
     3001 cccgtctatc ccgccgacct gctcggcagc atctaccacc tcggcggcct ggaccccaac
     3061 gccaaactgc cgcatcccat gggcctcgac gcttttgtgc tgccctccgc cgcggagggc
     3121 gtcaagtccg ccggcctgct caccgaaatc atgtagcccc cgccccgcgc ctgccatgcc
     3181 gctctccgct ctctctcgcg ccgccgccct cctggccgcc gcgctcctgg ccgccgcgct
     3241 ccccgcccgg gcccagcccc gcccctacgt cggcttcgtc tatcccgccg gcggtcagca
     3301 aggcactacc tttgccgtca aactgggcgg ccagaacctg aacgacgccc gcgaggcgcg
     3361 cgtcaccggc acgggcgtct ccgcccgcgt cgtcgaatgt tatcggcggc tcaacaacca
     3421 ggaaatgaac ctcctcaacg agcagctccg gcagctcaaa cgaaccacct ccgcctccga
     3481 cgccgccgtg atgaacgcgg acacccccat gatggcggcc cccctgacgc ccgcgcccgc
     3541 gtccgcttcg ccgcccggcg ccgcccccga ggccgccccc gccgccgccg accgccagct
     3601 catggcgcgc atcgaaaagc gcgccgccga atactgcaac cgccccgcca gcgccgccct
     3661 ggcctgcctg gtcttcctcg aagtcacgat cgcccccgac gcgcaacccg gcgcgcgcga
     3721 actccgcatc cgcacccccc tgggcctgtc caacccgatg gtcttccacg ttggccaact
     3781 gcccgagcat acccgccccc ccatgctcac cagcagccag caggtgctgg gcaaggaagc
     3841 cctctccctg cgccggcgcc cgcccgaaga agccctggtg cgcgtctccc tcccctgcgt
     3901 cctcaacggc caggtcgcct ccggcgaaat caaccaatac cgcttcaccg cccgcaaggg
     3961 ccagcgcctc gtcatcacca cccaggcccg ccagctcgtc cccttcatcg ccgacgccgt
     4021 ccccggctgg ttccagccgg tcctgctcct gcgcgacgcc gccggccggg aaatcgccta
     4081 ccaggacgac taccgcttca accccgaccc cacccttctc tgccaggtgc cccgcgacgg
     4141 agattacatc ctcagcatct acgacagcct ctaccgcggg cgcgaggatt tcgtctatcg
     4201 cctcagcctg ggcgagctgc cgttcatcac cagcatcttc cccctcggcg cctcaaccaa
     4261 cgcggcgcct cccatccgcg cccagggctg gaacctcgat agccaccggc tggcgccccc
     4321 gcccgccggc gccggccccg gcatccattt cctgaccgcg cgcaagcaag acctcctgtc
     4381 cccccccgtc cccttcgcgc tggacgccct gcccgaccgc gccgaacagg aacccaacca
     4441 gccccaggag cgcgcccagc cggtctccct gcccatcatg attaacgggc acatcaacca
     4501 gcccggcgac gaggacctgt tccgcttcac tggccgcgcc ggccagacca tcgccgccga
     4561 agtcttcgcc cgccgcctcg cctccccgct cgattccatc ctcaaactga ccgacagcgc
     4621 cggccgcctc ctggcctgga acgacgactg cgaagacttc gccgcgggca tcaacaccca
     4681 ccatgccgat tcctttctcc tggccaccct gcccgccgac ggcgactact gcattcatct
     4741 cggcgacatc gcccgccacg ccggccccga atacgcctac cgcctctgcc tccgcccggc
     4801 ccggcccgat ttcgacctcc gcgtggtccc ctccagcgtc aatctccgcc cccgatcctc
     4861 cgccgcgctc agcgtccacg tcctccggcg cgagggcttc accggcccca tcaaaatctc
     4921 cctcgccgac ccgccccccg gattcttcgc caacccggtc ttcctgtccg gcacccaaac
     4981 cgtcgcccgc ctgagcttaa aaacctcgct gcttgaaacc cccgaacccg tccgcctcac
     5041 cctcgccggc accgccaaaa tcggcaaccg ccaggtctcc cgcatcgccg tccccgccga
     5101 agaccgcatg caggcgttcc tctggcggca tctcgttccc gcttcggaac tcctcgccac
     5161 cgtttacaat cccgccgcgc cgcccccgcc cggacgcccc ctgcccactc tcccgcccgc
     5221 gccgctcgcc ggaaccacca ccaactcgtc cggcgacaaa cccaaattca cccggcaaca
     5281 aatcgccaga cgcctccgcg agttgaattc cctcttccag gaaaacctcc tcaccgaaga
     5341 cttctacctc cgcaaagtcg ccgagtgcga acccgccctc tgatcccggc gcctcccgtt
     5401 ccgggcacct gcccatcggc agacgcaact cccgcccccg cataggaacc caaaggccgc
     5461 aaaccatccc gcccctgttc ttcatcgcgc cgcgggagaa cgcgcagccg gcaaagggcg
     5521 cggagcactg cgcaggcgcg cgcggacggc aaggggacgc actgtgccgc gcggaaacac
     5581 gcagcgcgcc gaagcgccct cccgttcctt gccagccgcg
//
LOCUS       DAOPQG010000158         5434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_16468_length_5434_cov_23.538762, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000158 DAOPQG010000000
VERSION     DAOPQG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16468_length_5434_cov_23.538762"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PKI07_13075"
     CDS             complement(<1..834)
                     /locus_tag="PKI07_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52016.1"
                     /translation="MKVLTSPRTGSLGAETYYQSPFGVCARRRTVPRDRPSERKAAAR
                     SYFGISSREWGLKLSEAQRERWNAAALHVPSRPWMGQYSHLSGQQFCVQINSTLRGLG
                     LAPVEEPPAPVVFGPNPVTGLEIVNDAEGGVRLRLNVGATHEDIMVFGQPPCSAGRMK
                     PRRAYYLGLLGPSQNGQSDITDLYAARFGRPAPGQKVFIAVVQTRNGWKGQSRVHNAI
                     VPPPPMLKSQQAAETARVKKSKSVPAPASTAAHKTVSSPSFQAMYKGSTSEARQEYME
                     NK"
     gene            1150..1225
                     /locus_tag="PKI07_13080"
     tRNA            1150..1225
                     /locus_tag="PKI07_13080"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Thr,seq:cgt)
     gene            1370..1501
                     /locus_tag="PKI07_13085"
     CDS             1370..1501
                     /locus_tag="PKI07_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52017.1"
                     /translation="MTTLKMRVRERDWLIRRGNGENDETEYAGVLNKFVAIAGMESW"
     gene            1641..2174
                     /locus_tag="PKI07_13090"
     CDS             1641..2174
                     /locus_tag="PKI07_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOC52018.1"
                     /translation="MRAATIENPPPESRTVRPLARPLNALEVEIIDLFVQFSHILGQP
                     KSVAEIYGLLFAAAHPLSMEEMMERLNMSKGSTSQGLRFLRNLGAVKPVYVPGQRATH
                     FEAVAQLRTLAMRFLRDQVLTRLDNSLDRIDHMAALVENLPSEEREQVRARVGMLQSW
                     QKKTRKVLPVVMRTLGA"
     gene            2504..2809
                     /locus_tag="PKI07_13095"
     CDS             2504..2809
                     /locus_tag="PKI07_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52019.1"
                     /translation="MTPNDLLAGLPGEDLVRAGLSDLQAGRWTVPACRVVIAFPRMRR
                     AGLVREAAAATPADAELELYRMLRQTGGDAYSRYNALLRQLVSFARALDRRRRQCQP"
     gene            2806..4050
                     /locus_tag="PKI07_13100"
     CDS             2806..4050
                     /locus_tag="PKI07_13100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC52020.1"
                     /translation="MNRRSLRILALVPDAFGSHGGISKFSRDLLSALCAYPDCAEVVV
                     LPRLMPAAPGTLPAKLTWITTGLGGKVRFTAAAMRCARRQPSAPLAGAAGRNSQPSSG
                     FDLVICGHINLLPAAFLARRFVRRAPGSPNRGRCPLVLVMHGLDVWEPHRSVLVRSLA
                     RRVDAFVSVSAFTWDRFVRWSHVAAARQFVLPNCVDLSGFRPGEKDPVLLQKYGLAGR
                     KVVMTLGRLFRERPKGIDEVMELLPELAREAPRLAYLIAGDGPDRPRLADKARFLGLS
                     VLDCADSQAAIPGSPEPQVVFAGRVAESEKAAHYRLADAFVMPGYGEGFGIVYLEALA
                     CGIPVLASKLDASGEALRDGTMGLLVDPRNRDDLRRGLLQVLRQPRGQVPAGLTHFSH
                     PNYVRRCHAIVDTLCPAAAADA"
     gene            4093..4770
                     /locus_tag="PKI07_13105"
     CDS             4093..4770
                     /locus_tag="PKI07_13105"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOC52021.1"
                     /translation="MTAQEKPELPSETPPPACPSRRLRRLRSVVLWFCGLAIFFFLLF
                     VFRAPLLNGAARAWMVNDPPVKADAIVVLGGGAQFRSFEAARLYHAGLAPAILLMNSE
                     LRATDSMGLTIPEVELVRRILLTNAVPAAAIQIAGTNLTSTFEEAMAARHWSRASGAT
                     SLLIPTGSFHSRRVRWVFRKNLGDTVRITVTSIRPEECRDWWMDEKALLDFQNEFVKF
                     VLYRLRY"
     gene            4843..>5434
                     /locus_tag="PKI07_13110"
     CDS             4843..>5434
                     /locus_tag="PKI07_13110"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOC52022.1"
                     /translation="MKYLITGAGGFIGSHVVELLLNAGHEVRALAHYNSFGSWGHLEG
                     SPAAAHAKLEVRLGDVTDFSMMRTLVEGCDAVLHLAALIGIPYSYHAPASYVATNVSG
                     TLNVLEACRAASVRRVVITSTSEVYGTARYTPIDEKHPLQGQSPYSASKIAADKLAEA
                     YFCSFDLPVVTLRPFNTFGPRQSARAVIPTVLTQALN"
BASE COUNT          926 a         1660 c         1764 g         1084 t
ORIGIN      
        1 cttattttcc atgtactcct gccgggcttc cgatgtactc cccttgtaca tggcttggaa
       61 ggacggagaa gagacggtct tgtgggcggc ggtggatgcc ggggcgggga cggacttcga
      121 tttcttaacc cttgccgttt cagccgcttg ctgagatttc aacatcggcg gcggcgggac
      181 gatggcgttg tgaacccggc tttggccttt ccagccgttc cgggtctgaa ccacggcgat
      241 aaacaccttc tgacccgggg cggggcgccc aaaccgggcg gcgtagaggt ctgtgatgtc
      301 gctttgcccg ttctggcttg ggcccaacag gcccaggtaa taggcacggc ggggcttcat
      361 tcggcccgcg ctgcacggcg gctgcccaaa gaccataatg tcttcatggg tggctccgac
      421 gttcagccgc agccgaactc cgccttccgc gtcattgacg atctccaacc cggtgaccgg
      481 gttggggccg aagaccaccg gggcgggcgg ttcctccacc ggcgccagac ccagcccccg
      541 cagggtgctg ttgatctgga cgcaaaactg ctgcccactt aagtgcgaat actgccccat
      601 ccagggccgg ctggggacgt gcagcgccgc ggcgttccag cgctcgcgct gcgcttcgct
      661 gagcttgagg ccccactccc gcgaggagat cccgaaatat gaccgtgcgg ctgccttgcg
      721 ctccgagggt cggtcccggg gaacggtgcg ccgccgggcg cacacgccga agggggactg
      781 gtagtaagtt tccgcgccca gactgcccgt gcgcggagat gtgagtactt tcataatgtc
      841 tatcctttcc agttgttgct ctaattgttt ttctaaatgt ctttctgtta tcgccccgag
      901 gggcggctcg gcttcctgcc atttcttggg ctggaagtcc ccgtcgccac caccccgagc
      961 ccatgcggga aggctggcgg gcggggaggc ttcgcgcaag ggctattttg aaggggtggc
     1021 ggtggtgggc cgcaaagccc ctgatgaccg cggcatcatt tttagtatgc gcttgcattg
     1081 tcggagggaa gccgttagaa atagagagtc gccggtgtgt tccccacttc ggcgagcggg
     1141 cgccggattg ccaacgtagc tcagtcggta gagcagcgct ttcgtaaagc gcgggtcgtc
     1201 agttcgattc tgaccgttgg ctccagcccg aacgggttag gccgcaggag tttatggagg
     1261 aaaacggcca cgatggccac ctgaacggac cccgaaaagg cccgattctc ggcaagaact
     1321 gagggcttcc cgccgctagc cgattgctaa tgatgtgctc cggctcgctt tgacgacact
     1381 gaaaatgcgt gtgagagagc gcgactggct aatccggcgc ggcaatggtg aaaacgacga
     1441 aacggagtat gccggggtgc tgaataaatt cgtcgcgatt gctggaatgg aatcctggtg
     1501 atggacccgt aaagccgggc ggcttccgaa actcggctgc accgcggaca ggattcctta
     1561 tgggccggca agcttgagat aaggaaaaat atgttcataa taacttgaac aatcaggacg
     1621 ctatgctatg atttgttttt atgcgtgcgg ctactatcga aaatcctccg ccggaaagcc
     1681 gaactgtgcg gccgctggcc cggcctttga acgcgttgga agtcgagatc attgatctct
     1741 tcgtccagtt ttcacacatt ttgggccagc ccaagtcagt tgccgagata tacgggctgc
     1801 tttttgccgc cgcccacccg ctctcgatgg aggagatgat ggagcgtctg aacatgagca
     1861 agggctccac cagtcagggg ctgcgattct tgcgcaacct gggcgcggtc aagccggtgt
     1921 atgtgcccgg ccagcgggcg acccatttcg aggccgtcgc tcaattgcgc acgctcgcga
     1981 tgcgttttct gcgggaccag gttctgaccc gcctcgataa cagcttggac cgaattgacc
     2041 acatggcggc cctggtcgag aacttaccgt ccgaagaacg ggaacaggtc cgggcacggg
     2101 taggcatgct ccagagctgg caaaagaaga cgcgcaaggt cctgccggtt gtgatgagga
     2161 ccctgggagc ctgatccacc catttcatgt caccaacgat acgccagcag actcgagcct
     2221 cggcatccgg cctgcttaag ccgttcggac cgtggaagcg ggcaggatgc ccgcgttccg
     2281 gcctcgccgg catgaaatat ccgggctaga cgggaacgcg gtttcgggag ccggagcagt
     2341 tttgggaacg ggcgcggggg gataaccgcg caactggacc agcctgccgt ggcaggtgcc
     2401 agcgacatta ttgatatgcc gatcgggacg ttgatcggcg cgccgcaaag cgcgctgcga
     2461 attgacgggg cggagctttg gtcttgtggc ccgccaccgg cttatgaccc cgaatgatct
     2521 gctcgcagga ctgcctggcg aggacctcgt ccgcgcaggt ctgtcggacc tgcaagccgg
     2581 acgatggacg gtcccggcct gccgcgtggt catcgccttc ccgcggatgc ggcgggccgg
     2641 tttggtccgc gaggcggccg ccgccacgcc ggcggacgcg gaactcgaac tttatcggat
     2701 gctgcgtcaa actggcggcg acgcttattc ccgctacaac gcgttgctcc gccaactggt
     2761 cagcttcgcg cgggcgctcg accgccgccg tcgccagtgc cagccgtgaa ccgaagatca
     2821 ctgcggatac tggcgctggt tccggatgca tttggcagcc acggcggcat ttccaaattc
     2881 agccgggatc tgctaagcgc tttatgcgcc tacccggatt gcgccgaagt ggtggtgctg
     2941 ccgcggctga tgccggcggc gcccggcaca ttgccggcca aactgacctg gatcaccacg
     3001 gggctgggcg gcaaggtgcg cttcaccgcc gctgccatga ggtgtgcgcg gcgccagcct
     3061 tccgctccgc tcgccggggc tgccgggcgc aattctcagc cctcgagtgg ctttgatctt
     3121 gttatctgcg ggcacatcaa tctgctgccg gcggcttttc tggcgcggcg gttcgtgcgg
     3181 cgagcccctg gctctcccaa ccggggacgc tgcccgctgg ttctcgtcat gcacgggctg
     3241 gacgtctggg aaccgcaccg gagcgtgttg gtgcgaagcc tggcgcgccg ggtggacgcc
     3301 tttgtctcgg tgagtgcctt cacttgggat cgtttcgtcc gttggtcgca cgttgccgcg
     3361 gccaggcagt ttgtgctgcc gaattgcgtg gacctgagcg gttttcgtcc gggcgaaaaa
     3421 gacccggtct tgctccagaa gtacgggctg gctggcagga aagtggtaat gaccttggga
     3481 cgtttgtttc gggaacgccc caagggtatt gatgaagtga tggagctgct gccggaactg
     3541 gcgcgggagg ccccgcggct tgcgtatctg attgccggcg acggcccgga tcggccgcgc
     3601 ctggccgaca aggcccgctt tcttggactg agcgtgctcg attgcgccga ttctcaggca
     3661 gcaatccccg gttctccgga gccccaggtg gtcttcgcgg gcagggtggc cgagtccgag
     3721 aaagccgccc actatcggct ggcggacgcg ttcgtgatgc ccggctacgg cgagggattc
     3781 ggcattgttt acctggaagc cctggcgtgc ggcatcccgg tcctggccag caaactggac
     3841 gcgagcggcg aggctttgcg tgatggaacc atgggtttgc tggtggatcc gcgcaatcgt
     3901 gacgatctgc gccgcggcct gctccaggtg ctgcggcagc cgcgtggtca ggtgccggcc
     3961 ggcctgacgc acttttcgca tcccaactat gtcaggcgct gccatgcgat cgtggataca
     4021 ctttgtccgg ccgccgcagc cgacgcttga agcccctgcc tgggcgatac gcgagtgaaa
     4081 gcggatacac cgatgactgc gcaagagaag ccggagctgc cgtccgaaac ccctcccccg
     4141 gcttgcccca gccgtcggct gcgccggttg cgatccgttg tcctgtggtt ctgtggtctc
     4201 gctatctttt tcttcctgct ttttgtgttc cgcgccccgc tgctcaacgg cgcggcgagg
     4261 gcctggatgg ttaacgatcc cccggtcaag gccgatgcga ttgtcgtgct gggcgggggt
     4321 gcccagttcc ggtcgttcga agccgcccgg ctgtatcatg cgggactggc gccggcgatc
     4381 ctgttgatga actccgaact ccgcgccacc gacagcatgg gcttgaccat cccggaggtg
     4441 gaactggtgc ggcggattct gctcacgaac gccgtgccgg cggcggcgat acagattgcc
     4501 ggcaccaatc tcacgagcac tttcgaagag gcgatggccg cgcggcattg gtcccgcgca
     4561 tcaggcgcca cctccttgct gattcccacc ggctccttcc attcccgccg cgtgcgatgg
     4621 gtgtttcgca aaaacctggg cgacacggtc cggattacgg tcaccagcat ccggccggaa
     4681 gaatgccggg actggtggat ggacgaaaag gcgctgctcg attttcagaa cgagtttgtc
     4741 aagttcgtcc tttaccggct gaggtactaa aaggaagagc aacgagcctg ccgacaacaa
     4801 gttctcgggc tacaagacca caggcaagag gaattccgac acatgaaata cctgattacc
     4861 ggcgcgggtg gattcatcgg cagtcacgtg gtggagttgc tcctcaacgc ggggcacgag
     4921 gtgcgggcgc tggcgcatta caacagcttc ggcagttggg gccacctgga aggcagcccc
     4981 gctgccgccc acgcgaagct cgaggtccgg ttgggcgacg tcaccgactt ctcaatgatg
     5041 cgtactctgg tggagggctg cgacgccgtg ctccacctcg cggcgttgat cggcattccc
     5101 tattcgtacc acgcgcccgc ttcgtatgtg gcgacgaatg tcagcggcac tttgaacgtt
     5161 ctcgaagcct gccgcgcggc aagcgtccgg cgggtggtca tcacctccac cagcgaggtt
     5221 tacggcacgg cgcgctacac gccgatagac gaaaagcacc cgcttcaggg gcaatcccct
     5281 tattcggcca gcaagattgc ggcagacaag ctcgccgaag cctatttttg cagcttcgac
     5341 ctgccggtcg tcacgctgcg ccccttcaat acctttggtc cgcgccaatc cgcccgggcc
     5401 gtcatcccca ccgtcctgac gcaggcgctc aacg
//
LOCUS       DAOPQG010000159         5220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_17313_length_5220_cov_13.208325, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000159 DAOPQG010000000
VERSION     DAOPQG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17313_length_5220_cov_13.208325"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..2147)
                     /locus_tag="PKI07_13115"
     CDS             complement(162..2147)
                     /locus_tag="PKI07_13115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC52023.1"
                     /translation="MKRNNQGMFILPDGRNVLFTFCLVSSLFLLWGFCNGMIDVMDKH
                     FQEELHLSLSQSAWVQFAHWMGYFLMSIPAGWLATRLGYKGGIIAGLLLVAVGGFWFI
                     PASKIAAFWAFLLGVCVVASGLTFLETVANPYTTVLGPPRYAATRINTAQSCNGVGWI
                     FGPIAGSLFFYGTDAAGRSTGSQTLWIPYAGVAVGVLILAIIFFFAPVPDIKAEDEYR
                     QDDKDNHAATPATPAAREVNRGLSYVLLLGNVAALIGVFIFIFWLTFEGLHFGPKLVG
                     LASAIPHPPGMIVNDQNSVLIVLVAAAGLALAAAAIWLIGVTKRLSGHSVWAREHFSG
                     ATLAQFFYVAAQCGIFSFLINYMTSEPPSLPASWLKESTGKWVEVRTAFAGADFKDVS
                     TLAAKLTAQADPVSAFLTANLSEATRQTLGRFQEGGVSTTTARVAIMQDLNSLILKTN
                     IYAPERFAGVTLQPKTAQLLAPDAPPRSLPRLNRLLLTDAYPQELAFQDGIFGVSNQF
                     AAKLASVGFFCFLLGRVTGAAMLRKVSAHKMLGLYSMINAILCFLIFLKLGWLSVVCV
                     FLCYFFMSITFPTIFALGIFGLGARAKSASAYIVMGIVGGALLPKLMGAVADHYNMSR
                     GFIVPLICFVFIALYGYGWPKLSRVKALHGVGASGSH"
     gene            complement(2253..3440)
                     /locus_tag="PKI07_13120"
     CDS             complement(2253..3440)
                     /locus_tag="PKI07_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52024.1"
                     /translation="MTTTGTIPHHAGAASYGDDAAFLKRHTEVIELSDAKGLAKVAVV
                     SAWQGRVMTSTAGADAGLSFGWINRELIASGKLLPHMNAFGGEDRFWMGPEGGQFSIF
                     FAKGVKFEFENWFTPAAFDTLPFKTLRHSRTKAVFASEFMLTNYSGARFEVAVNREVR
                     LLNTKAAWQKLGVPPAAEVSVVAYESDNKITNRGKHAWQKNTGLLSIWILGMFTPSPS
                     ATIVVPIKRGPESELGVKVTSDYFGQIPPERLVVRDDVIFFSADGKYRSKIGINPKRA
                     RGVSGSYDADNKVLTIAQYTLPSGATDYVNSLWKLQDNPYAGDAANCYNDGPPAPGAK
                     PMGPFFELESSSPAAALAPGKSLSHVHRTIHLRGPEAALDAVARRVLGVSIADIKTAL
                     KTR"
     gene            complement(4268..4474)
                     /locus_tag="PKI07_13125"
                     /pseudo
     CDS             complement(4268..4474)
                     /locus_tag="PKI07_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
BASE COUNT         1195 a         1408 c         1548 g         1069 t
ORIGIN      
        1 caccttgagt ggaaaccatg acgaccaatt tgctgaactt gacggacaca actcgggatc
       61 gttggtgcga caagtcttac ggcgcgtata cggaacgagc gcaataactc cgcttgcgag
      121 actgtatttt gttttacccg cgaagcgttg atttccacaa atcagtgact accgctggca
      181 cccacgccgt gaagcgcttt gacgcggctc agcttgggcc agccataacc gtagagagcg
      241 atgaacacga agcaaatcag cgggacgatg aacccgcgcg acatgttgta atgatcggcg
      301 acagcaccca tgagtttggg aagcagggcg ccgccgacga tgcccatgac gatgtaagcc
      361 gaagccgact tggcccgtgc ccccaacccg aagatgccga gggcgaagat agtgggaaaa
      421 gtaatggaca tgaagaaata gcacaggaag acgcatacca ccgatagcca gcccagcttc
      481 agaaagatca ggaaacagag gatggcattg atcatactgt agagccccag catcttgtgg
      541 gcggaaacct tccgcagcat ggcggctcct gtgacccgcc cgagaaggaa gcagaagaac
      601 cctacggacg ccagtttggc cgcaaattgg ttgctcacgc cgaaaatacc gtcctgaaag
      661 gccagttcct gcggataggc atcggtcagc aataaccggt tgagccgcgg cagactgcgc
      721 ggcggagcgt ccggcgcgag cagttgggcg gtcttgggtt gcagcgtgac tccggcaaat
      781 cgctccgggg cgtagatatt ggtcttgaga atgagactgt tgaggtcctg catgatggct
      841 acgcgcgccg ttgtggtact gacgccgcct tcctgaaagc ggcctagggt ttgccgggtt
      901 gcctcagaga ggttggcggt gagaaaagcc gacactgggt cagcctgagc tgtaagtttg
      961 gccgccaggg ttgacacgtc tttgaaatcg gcgcctgcga aagccgtccg cacctcgacc
     1021 catttgccgg tgctttcctt aagccaggaa gctggcagtg aggggggttc ggaagtcata
     1081 tagttgataa ggaaactaaa aatgccacac tgggcggcaa catagaagaa ctgggccagg
     1141 gtggcgccgg aaaagtgttc gcgcgcccag acactatgcc ccgagaggcg tttggtaacc
     1201 ccgatcagcc agatcgccgc agcggccaaa gccaggccgg cggcagcaac caacacgatg
     1261 agcaccgagt tttggtcatt tacgatcatg ccgggcggat gcggaatggc cgaggccagc
     1321 cctaccagtt tggggccgaa atggaggcct tcaaaggtca gccaaaagat gaagatgaag
     1381 acgccaatga gcgccgcgac attccccagc agcagaacat acgacagccc gcggtttacc
     1441 tcccgggccg ctggggtggc aggcgttgcg gcatggttgt ctttatcgtc ctggcgatac
     1501 tcgtcctcag ctttgatatc cgggaccggg gcaaaaaaga agatgatggc caggatgaga
     1561 actcccacag cgactcctgc gtaggggatc cagagtgttt gagaaccagt gctccgcccg
     1621 gcggcgtctg tgccgtagaa gaacaggctg ccggcgatgg ggccgaaaat ccagccgaca
     1681 ccattgcagg attgggcggt attgatccgc gtggcggcgt agcgtggggg ccccagcacg
     1741 gtggtgtagg ggttggcaac ggtttccaga aaggtcagcc cggacgccac cacgcacacg
     1801 ccgaggagga aagcccagaa cgcggcgatc ttcgacgcgg gaataaacca gaagcctccc
     1861 acggccacca acagcaaccc ggcgatgatg ccgcctttat agcccaggcg ggttgccagc
     1921 cagccagcgg ggatggacat gagaaaatag cccatccagt gggcaaactg cacccaggca
     1981 gattgggaca ggctcaaatg caattcctcc tgaaaatgtt tgtccattac atctatcatc
     2041 ccattgcaga agccccacag gaggaagagc gaactaacga gacagaaagt gaacagaaca
     2101 ttccggccat ccggcaggat gaacatgcct tggttattgc gtttcatggg agtggactga
     2161 tttgttaaag accaggagcc agtgcggcca gtgccttggt ttcgtgtagc ttggcaggtg
     2221 gctgggattg cggaacgctt tttccctgtt catcaccggg ttttgagcgc cgtcttgatg
     2281 tcagcaatcg agacgcccaa cacgcgccgc gccacagcgt caagcgccgc ttccggtccg
     2341 cgcaaatgaa tggtgcggtg aacatgcgaa aggcttttgc cgggagccaa cgcggcggct
     2401 ggggaggaag attcgagttc gaagaaaggg cccatcggtt tggctcccgg agcgggtggc
     2461 ccgtcgttgt agcaattggc ggcatcgccg gcgtaggggt tatcctggag tttccaaagc
     2521 gaattgacat aatcagtcgc gccagagggc agcgtatatt gggcgatagt cagcaccttg
     2581 ttatccgcgt cataactgcc cgacacgccc cgggcacgct tggggttgat gccgattttg
     2641 ctgcgatact tgccatccgc actgaagaag atcacatcgt cccggacaac gaggcgttcc
     2701 ggaggaatct ggccgaaata atcggaagtg accttcaccc cgagttcaga ctccggcccg
     2761 cgcttgatcg ggaccacaat ggtagccgaa ggcgaggggg taaacatccc cagaatccag
     2821 atggaaagca ggccggtatt cttttgccac gcatgcttgc cgcgattggt gatcttatta
     2881 tctgattcat aggcaacgac gctaacttcc gcagcaggcg gcactcccaa cttttgccag
     2941 gcggccttcg tattgagtag acggacttcc cgattcaccg ctacctcgaa ccgggcaccg
     3001 gaataattgg tgagcatgaa ctcggaagca aacaccgcct tggttcggga atggcgcagg
     3061 gtcttgaatg gcaacgtgtc gaaagcagcc ggcgtaaacc agttctcgaa ctcaaacttc
     3121 actcccttag cgaagaatat tgagaattgt ccgccttcgg ggcccatcca aaaacggtcc
     3181 tccccgccaa aagcattcat gtgcggcaat agcttgcctg aagcgatcaa ttcgcggttg
     3241 atccagccaa aactaagacc tgcgtctgca ccggccgtgc tggtcataac gcggccttgc
     3301 caggcggaca ccacggccac tttggcgagg cctttcgcat cgctgagttc gataacctcg
     3361 gtatggcgct tcagaaaggc ggcatcgtcg ccataactgg ctgcgccggc gtggtgaggg
     3421 atggttccgg ttgtggtcat aactacaatc gtagcaaaag tcagattgcg caacatgagc
     3481 caagcctacg tactggcgcc cggcggtgtc aagcagggcg cagggggcag gcaaaatcgt
     3541 tacgcattgg caacggggtt gcttcggctc ttatcccccc ctgcgagaat agaaataagg
     3601 ccgtggcgcc accgattcaa gggagaatgg ttgcgtgcca ttcgtcggtg ccgcgaaacc
     3661 atttccagag atacaacttt cggggaaccc attccgcgtg gccatcgcaa aacacgacat
     3721 tgccaccggc agtgccgtga ttgtctccga catccgggta atcctcattt tggcgcgagg
     3781 ggtctgaggc caatcggtcg tccgcatcat agatgatcca gatatccgca gggcccccgc
     3841 tttggccgtt gaagttatat tcggggaatc cgcccgcggc attgttgagg cggtaggtat
     3901 aactggccac ggcagtttgg gttttccgga tcatcgcacc gccgacgccg gacgactggc
     3961 gtgcattaat ccacccggca acttcataac tgtgcccaat ggttcccgct ttcccctcgg
     4021 cgttattaac aaggtcgggc aggtatttcg tgccgccatg caggcgttcc tgataactgt
     4081 ttacccccga gtcgttgggg gtgtagggac tgggagtagt ggccgtgacc tcccgcgcat
     4141 tggtgaagcg aatattgttt ttagtcgtgg ggcaaataaa ggacttaata gtcgcaaggt
     4201 atagagggaa cagccagttc aggtcgtcgt cggtccagtt ggcggtgccg cttagagcgc
     4261 gtttgtcgtc gtcatccgca tagatctggc tgccgagacc catctgcttg ctgttgttaa
     4321 ggcaggcggt gcgccgggcg cgattctgcg cctggccgag agctggcaag agcaaggcgg
     4381 ccaggattgc gatgatggct atgacgacca ataattcaat caaggtgaat cctaaacgcg
     4441 ctgcgccgcc tctctttgag ccatacgagt tcatttgatt tagcaggagc tattgaaaac
     4501 ctgctgcttt tttgaataac acacccgtga aagatctgca acctaacttg gccctggatc
     4561 acgaagctgg caaccttgat atccggcagg ccatccatag aatcagcatt tgaaacatgc
     4621 ctgattccga atttgccctg cgcctgaggc tctttagcgg cgaaatgctg ctgctagggg
     4681 tggcagtgct gcttgcgctt gttttccttg gcgcggtggt ctttgatctc attaaacgtc
     4741 gtcgccggaa acggcgtcgg tcgttcgcat cgagagaacg ccgagggcta ttgcgtgggg
     4801 ttttcaatcg catgcggact ttgcgaaacg aattaaggaa agtgcttcgc cgacgttccc
     4861 gacaaactaa acggcatcgg agcaaacacc gttcgtcccc acgggtatag ttctcatctc
     4921 caaagggatc cttcgttttc ccaatcagcg cctgggcgca tcgcttccac tgtcatgctt
     4981 cgcccatcca gagtaatcag ccgcgtacaa cttctggaac tacggcttgc aaaaacgtaa
     5041 aaacgaaggc aaatccagtt tggataacgg attttgcgct atccaagcgg aggctatcgg
     5101 tttaaaaagg gcgtatgcct gtgctggtta agggcggtga aatcatcacc gctacggaac
     5161 gctactgtta tgtaacagat aactctttac aatgtcgttt ggcgtggtat gctgggcggc
//
LOCUS       DAOPQG010000160         5203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_17392_length_5203_cov_11.552448, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000160 DAOPQG010000000
VERSION     DAOPQG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17392_length_5203_cov_11.552448"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..295
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..32
                     /rpt_unit_seq="tgccttgattccaaaaccggggggggcatact"
     gene            complement(327..662)
                     /gene="cas2"
                     /locus_tag="PKI07_13130"
     CDS             complement(327..662)
                     /gene="cas2"
                     /locus_tag="PKI07_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOC52025.1"
                     /translation="MDTWKFKMGWLIVAFDLPVGTRPQRARAARFRKFLLDDGFLMIQ
                     LSVYARACVSFARQETHLARVKNNLPPEGSVRAVYVTRAQWERSFVIQGAPAKTQKAE
                     DLPEQIQLW"
     gene            complement(629..>1631)
                     /gene="cas1"
                     /locus_tag="PKI07_13135"
     CDS             complement(629..>1631)
                     /gene="cas1"
                     /locus_tag="PKI07_13135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOC52026.1"
                     /translation="SAPAGRAPGGPAARFGTELALLPCRTGRMSYHIVSIHSPQCVLS
                     CRDGQLTCQAADGERKLPLEDIAAIIITSFAAQIHSELLLAAARHGVALILCEAFKPA
                     SLVLPANRSTDTTLSRALVRLSPGARRQLWERTLDAKCRNQHALAARIAPADPQTAAL
                     ARAARSRRPHREAAGAKALWRVFGRALGEPRFTRARGGGGLNDLLNYGYAVLLSTVLQ
                     KLFAVGLDPTFGVSHLPRERCTPLAYDLMEPFRPCVDGRVFQWAREHPRRADWTVSPE
                     YRKWAARLPLEPVPYRGGSLAIQNCIEAVVRSFRRAVLAGRPALYHPWTPGNSRWDG"
     assembly_gap    1632..1731
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1732..4999)
                     /locus_tag="PKI07_13140"
     CDS             complement(<1732..4999)
                     /locus_tag="PKI07_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOC52027.1"
                     /translation="MSAKQNISSPHPDLHLAFDVGHSSIGWAVLQTPAAPSPEPAAEL
                     LGCGAVVFAPDDCLASTRRGFRRQRRHIRATRQRIARMRRLLAHLGVLTPAQLAAPGG
                     AWPWLLAARVLGGGAPLTWPELWDVLRWYAHNRGYDANRAWSRQEKPDTEDSEKAQRA
                     HELLREFKRRHGRPGTMAEVFCDHLGVDPRGPVKSSRQRVRHLGAAFPREGVEAEVER
                     ILQAHKDILPGVDDPLIHALLRDHTARPVPGLRLPARYGQRQPDGTLRPGGLLFGQLL
                     PRFDNRIIARCPIAYERVYQQVLAETGDREKARREAEKRAKVPAAHDADFHRFRWAMV
                     LAQINIAVPGAPQPRNLSAPERNSLHAVMEQKGGLTKSELTQAVRQLTGRAPDNLDRL
                     LALPDAERALVLDPVRLYCHRDPLLSVLWPALPARLQRRLRGQWRRGRALTVADVLAA
                     CGDARPAAEAALAQYLEAEAAKKKRAPDAPRIPDTPAGRVLRVERARGRAPHTREIMR
                     EAVAFVLATDRHPAEEGGPLYRSDAIREAQLRRALDEQTNNHLIRHRLKLLAGDDAAG
                     PRALNGLFRDLANTYAGGDLGRLGRITIEVNRDLRELSGKTAQEQAQLIGQQLANFKS
                     VEKKLTEAYAGLGVRVDPGLIRKGRIAEDLGWTCPYTGQPYDALALLHRRVDKDHIIP
                     RSERASDSLDSLVITFAEVNKWKGQRTALRFIEEEQGKPVPGLPQLSLRTLAAYRQAV
                     EKLECFKGHENDQRRKRNRKRRLLLRHYVEKDFTPADLTQTSQLVRLCAQALQRLGLG
                     CARPPVVTALPGAVTAAVRKAWNLTGCLAPANPLALNPDDLDDHGQPRPHNKTELRKI
                     THLHHALDACVLALAAHHLPRDGEAWRLLLKRRLAPDEQRRARQLLGRVAAITPQGEL
                     RLNDLPPALKEQVSRRLRERRVVQHIPADLSGLRCDETVWRVFDPADPHPNARRLARW
                     LANKNIPIPAPDAPTVLIISRKRNGADAAPAAGKVFHQTPTWRWVYEIKDKTALLGLA
                     PEGDPAAAKLKRLKAVKVLGDNFGLALDPEPALIRPHKIWHQLAALRARNGGKPPRVL
                     R"
BASE COUNT          665 a         1550 c         2052 g          836 t
ORIGIN      
        1 tgccttgatt ccaaaaccgg ggggggcata ctccgtcgag cagaacggct atgccctgcg
       61 ctgctttgcc ttgattccaa aaccgggggg ggcatactgt agtggacgag aggcgcgagg
      121 aaaggacagc tttgccttga ttccaaaacc ggggggggca tactggcggt ggcgcggggg
      181 tttgggcggt atagctttgc cttgattcca aaaccggggg gggcatactt agtcccgcgc
      241 ttgatcccta ccacttctcg ctttgccttg attccaaaac cggggggggc atactaggtg
      301 gtggtaaccc gctcgtggag agcgggttac cacagttgta tttgttcggg caggtcttcg
      361 gccttctggg ttttggcggg ggcgccctgg attacgaagg agcgttccca ttgggcgcgg
      421 gtgacataga cggcgcggac gctgccctcc ggggggaggt tgtttttgac gcgggccagg
      481 tgggtttcct gccgggcgaa ggagacgcag gcgcgggcgt acacgctgag ctggatcatc
      541 agaaagccgt cgtcgagcag gaacttgcgg aagcgggcgg cccgcgcgcg ctgcgggcgg
      601 gtgccgacgg ggaggtcaaa ggcgacgatc agccatccca tcttgaattt ccaggtgtcc
      661 acgggtggta aagcgccggg cggcccgcga ggacggcgcg gcggaagctg cgcacgacgg
      721 cctcgatgca gttttggatg gcgagggagc cgccccggta aggcaccggc tcgaggggga
      781 ggcgggccgc ccatttccgg tattccgggc tgacggtcca gtccgcgcgg cgggggtgct
      841 cgcgggccca ctgaaacacg cgcccgtcca cgcaggggcg gaacggctcc atcaagtcgt
      901 aggccagggg ggtgcagcgc tcgcgcggca ggtgggagac gccgaaggtg gggtccagcc
      961 ccacggcgaa cagtttttgc agcacggtgg aaagcaggac ggcgtagccg tagttgagca
     1021 ggtcgttcag cccgccgccg ccgcgcgcgc gggtgaaccg cggctccccc agcgcccggc
     1081 cgaacacccg ccagagggct ttggcgccgg cggcctcgcg gtgcgggcgg cggctccggg
     1141 cggcccgcgc cagcgccgcc gtttgcgggt cggcgggggc gatccgggcg gccagcgcgt
     1201 gctgattgcg gcatttggcg tccagcgtcc gctcccaaag ctggcggcgc gcgcctgggc
     1261 tcaggcgtac cagcgcccgg gagagggtgg tgtcggtgga gcggttggcg ggcagcacca
     1321 ggctggcggg cttgaacgcc tcgcagagga tcagggccac gccgtgccgg gcggcggcca
     1381 gcagcagctc gctgtggatt tgcgccgcga agctcgtgat gataatggcg gcgatgtcct
     1441 ccagcgggag tttccgctcg ccgtccgccg cctggcacgt cagttgcccg tcccggcagc
     1501 tcaagacgca ttgcggactg tggatgctga caatatggta cgacatgcga cccgtccggc
     1561 atggcaaaag agccagttct gtgccaaaac gggcggccgg gccgcccggc gcgcgcccgg
     1621 ccggggctga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgcgcaaga
     1741 cgcgcggggg tttgccgccg ttgcgcgcgc gcagggccgc gagctggtgc cagattttgt
     1801 ggggccggat gagggccggc tccgggtcca gcgccaggcc gaagttgtcg ccgagcactt
     1861 ttaccgcttt gaggcgtttg agtttggcgg cggcggggtc gccttccggg gccagcccca
     1921 gcagggcggt tttgtctttg atctcataga cccagcgcca ggtcggggtt tggtggaaga
     1981 ctttgccggc ggcgggggcg gcgtccgcgc cgttccgttt gcggctgatg atgaggacgg
     2041 tgggggcgtc gggggcgggg atggggatgt ttttattggc cagccagcgg gcgaggcggc
     2101 gggcgttggg gtgggggtcc gcggggtcga agacgcgcca gacggtttcg tcgcagcgca
     2161 ggccggagag gtcggcgggg atgtgctgga cgacgcgccg ctcccgcagg cgccggctga
     2221 cctgctcttt gagcgcgggc ggcagatcgt tgaggcgcag ttcgccctgg ggggtgatgg
     2281 ccgccacgcg ccccagcagt tggcgggcgc ggcgttgctc gtcgggagcc aggcggcgtt
     2341 tgaggaggag cctccacgct tcgccgtccc ggggcaggtg gtgcgcggcc agggcgagga
     2401 cgcaggcgtc cagggcgtgg tggaggtggg tgattttgcg cagctcggtt ttgttgtggg
     2461 ggcggggctg gccgtggtcg tcgaggtcgt ccgggttgag ggccagggga ttggccgggg
     2521 ccaggcagcc ggtaaggttc caggccttgc ggacggcggc ggtgacggcg ccgggcaggg
     2581 cggtcacgac cggggggcgg gcgcagccga ggccgagccg ctggagcgcc tgcgcgcaca
     2641 ggcggacgag ctgcgaggtc tgggtcaggt cggcgggggt gaagtccttt tcgacgtaat
     2701 ggcgcaggag gagccgccgt ttgcggttgc gcttgcggcg ctggtcgttt tcatggcctt
     2761 tgaagcattc cagcttttcg accgcctgcc ggtaggcggc cagggttcgg aggctcagtt
     2821 ggggcaggcc cggcacgggt ttgccttgtt cctcctcgat gaagcgcagg gcggtgcgct
     2881 ggcctttcca cttgttgacc tcggcgaagg taatcaccag ggaatcgagg ctgtcgctcg
     2941 cgcgctcgga acgggggatg atgtggtctt tatccacccg gcggtggagg agggccaggg
     3001 cgtcgtaggg ctggccggtg taggggcagg tccagcccag gtcttcggcg atgcggcctt
     3061 tgcggatcag ccccgggtcg acgcgcacgc ccaggcccgc gtaggcttcg gtgagctttt
     3121 tttccacgct tttgaagttg gccagttgct ggccgatcaa ctgggcctgc tcctgcgcgg
     3181 ttttgccgga cagctcgcgc aggtcgcggt tcacctcgat ggtgatgcgc ccgaggcgcc
     3241 cgaggtcgcc gccggcgtag gtgttggcca ggtcgcggaa cagcccgttg agcgcgcgcg
     3301 ggcccgcggc gtcgtcgccc gcgagcagct tgaggcggtg gcggatcagg tggttgttgg
     3361 tctgctcatc cagggcgcgc cggagctggg cttcccggat ggcgtcgctc cggtagagcg
     3421 gcccgccctc ctccgccggg tggcggtcgg tggccaggac gaaggccacg gcttcgcgca
     3481 tgatctcgcg ggtgtgcggc gcccggcccc gggctctttc cacgcgcagg acccggcccg
     3541 ccggcgtgtc ggggatgcgg ggggcgtccg gggcgcgctt tttcttcgcg gcttccgctt
     3601 cgaggtattg cgcgagggcg gcttcggcgg cggggcgggc gtcgccgcag gccgccagca
     3661 cgtccgcgac ggtgagggcc cggccgcggc gccactggcc gcgcagccgc cgttgcaggc
     3721 gggcgggcag cgccggccag aggacggaca gcagcgggtc gcggtggcag tatagccgca
     3781 ccgggtcgag gaccagggcg cgctcggcgt cggggagggc gaggagccgg tcgaggttgt
     3841 ccggcgcgcg gccggtcagt tgccgcacgg cctgcgtgag ttcgcttttc gtcaggccgc
     3901 ctttttgctc catcaccgcg tggaggctgt tgcgctcggg ggcggagaga ttgcggggct
     3961 ggggcgcgcc gggcacggcg atgttgattt gggccagcac catggcccag cggaagcggt
     4021 ggaaatcggc gtcgtgggcg gcgggcacct tggcgcgttt ctccgcttcg cggcgggcct
     4081 tttcccggtc gccggtttcg gccaggactt gctggtagac gcgttcgtaa gcgatcgggc
     4141 agcgggcgat gatgcggttg tcgaagcggg gcagcaactg gccgaagagc aggccgccgg
     4201 ggcggagcgt gccgtcgggc tgccgctggc cgtagcgcgc gggcagccgg aggccgggaa
     4261 cgggccgcgc ggtgtggtcg cgcaggaggg cgtggatgag cgggtcatcc accccgggca
     4321 ggatgtcctt gtgggcctgg aggatgcgct cgacctcggc ttccaccccc tcgcgcggga
     4381 aggccgcgcc caggtggcgc acgcgctggc gggaggattt gaccgggccc cgcgggtcca
     4441 cacccaaatg atcgcagaag acctcggcca tggtcccggg gcggccgtgg cggcgcttga
     4501 actcccgcag caattcgtgg gcgcgctggg ctttctcgga atcctccgtg tcgggcttct
     4561 cctgccggct ccaggcccgg ttggcgtcgt agccgcggtt atgggcatac cagcgcagca
     4621 cgtcccacag ctcgggccag gtgagcggcg cgccgccgcc cagcacgcgg gccgccagca
     4681 gccacggcca cgcgccgccg ggcgcggcca gttgcgccgg ggtcagcacc ccgagatgcg
     4741 ccagcaggcg ccgcatccgc gcgatgcgct gccgggtggc gcggatatgc cgccgctgcc
     4801 gccggaatcc gcgccgcgtc gaggccaggc agtcatccgg cgcgaagacg acggcgccgc
     4861 atcccagcag ctccgccgcg ggctccgggg agggcgccgc gggcgtttgc aggaccgccc
     4921 agccaatcga gctgtggccc acatcgaagg cgaggtgcag gtcggggtgg ggagaggaaa
     4981 tattttgctt ggcactcatg atcagggggg gttagattgg aggcggaatc aagacaaagt
     5041 tagataacgc aagctggttc cctatccgcc cggcggatac accagttgcc cttgaggcga
     5101 aagcgtttct gcattcaaaa ccggcggggg ccatccccgc cggttttgcg tgagcgggac
     5161 gggcgggcgc cggcgttccg cgcgcgccgc gctcccgcgc cgg
//
LOCUS       DAOPQG010000161         5176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_17517_length_5176_cov_16.966413, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000161 DAOPQG010000000
VERSION     DAOPQG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17517_length_5176_cov_16.966413"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1012..2082
                     /locus_tag="PKI07_13145"
     CDS             1012..2082
                     /locus_tag="PKI07_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOC52028.1"
                     /translation="MATPLDGGEVNLGNLYRVSKAESRSISPENFTGEKGKAGMATEG
                     TGKGPARDLGQGWKVSPSVRIKAGTTFTLGEINGPGLIQQIWMTPTGNWRRSILRFYW
                     DDEAEPSVECPVGDFFACGWGKYCQVSSLAVCVNPGSAFNCYWPMPFRKKARITMENR
                     DAQDMILYYQINYALAPVPKSAAYFHAQFRRESPLKQKGLYTILDGVAGQGHYVGTYL
                     AWEVHSPGWWGEGEIKFFMDGDRQFPTICGTGTEDYFCGSYNFENRETKKYQVFCTPY
                     AGLAQVLPPDRIYEVGQRFGLYRWHIPDPVRFKKDLKVTIQALGWQEGGRYLPLEDDI
                     ASVAYWYQKEPHQAFPKLPPNL"
     gene            2316..3287
                     /locus_tag="PKI07_13150"
     CDS             2316..3287
                     /locus_tag="PKI07_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC52029.1"
                     /translation="MNITPSCSRRSFIKTTGLALSAGMLSTASPLLAAGKKKTLVGCR
                     DVHLKVAGLPDSWSSMKAVGAEAIEVCVELDLTCPLLFHPQRKYTLATPEGIRTLKDD
                     LSASGCRIAAFMMGNRFDERLEEELECARRLIPAAQQLGVEIIRIDVVPRKLEGDQFL
                     SFAIDTCKRLCALAEDSPIRYGVENHGKITNDPQFLDRLFDGVGSCRLGLTLDTANFY
                     WWGHPVSELYPIYEKFAPRVVHTHCKSIGYPDDKKNVRREMGWEYGKYCCPIYEGDLD
                     FARIVKILRKARYRGDLCVENESLGRFPENQRADILRREIALLKKLA"
     gene            3416..4921
                     /locus_tag="PKI07_13155"
     CDS             3416..4921
                     /locus_tag="PKI07_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC52030.1"
                     /translation="MILGLSLPLAVLVGYFLAEPMELGSAAVVLAVVGVLSVPLLMRW
                     YHPVLIFLWNSAINPVVLPGRAGLWMVVTCIALLFAVLNRAVNPKARFIMVPAVTYPL
                     LALGVLVVITAVLRGGAGFASLGSSQYGGSRYANLLMGIAGYFALTSRRIPPSRAGLY
                     VGCFFLSGLTYAISDLAILAGRQFYFLLYIFSTTYATDQLKYLETFNPSIIRFRGTSV
                     VASAIYTYLLARFGIRGILDVARPWRGLIFLLAVAGGMGGGFRSYVALFGLTCIGVFY
                     LEGLHRTRYLPALLAVALVGGALVLPQADKLPLAVQRGLVFLPGKFDSLARQSAKTSI
                     EWRVEMWKRVWPEVPKYLFLGKGYKLDPSELFLAQYFSPRYHDAMDSGSQVAGDYHSG
                     PLSVLVPFGIFGTLAFLWFLVAGTRTLYLYYKHGSPVYQTVNTLLLAAFAARAIFYFV
                     GFGSLHTDMAFFTGLLGLGVALNGTPSAAAASSPSEQPDTGVELNTEYVKV"
     gene            4944..>5176
                     /locus_tag="PKI07_13160"
     CDS             4944..>5176
                     /locus_tag="PKI07_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52031.1"
                     /translation="MSNASALFRSLIVYGLCLPLAVALGYLLANPLDLTTVVVVGSLC
                     FILMIPIFLRWHHVWLIAAWNMSAVVFFLPGRPP"
BASE COUNT          933 a         1434 c         1542 g         1267 t
ORIGIN      
        1 ttataagtcc gaagctgatg ggagatgatt ttccaaaggc gggtgggcgc tcggatgact
       61 gttggttttg cgaatgactt atttgcagtg ttgccgttgc atagtccagc cgagtgccct
      121 catttcactg aatccaaaac gggactgata agccaatggg cagccgcaac gagccgtttg
      181 attgatgaat tcaacgatct tgccgttgac ttccgaatat ttttctcccg taaaaccaag
      241 agattacgtg tcctgtgagg tcgtttctct gcctccggtc tttagccagg gactcttatt
      301 ggcaaaaggg agtaattgct tgatacagga taaaaaactt tttgggttct atatcgttaa
      361 cggtaatcgc taaacatgtt agattaacgg ttatttgcgt tttttgaccc tgtttcatga
      421 agaaacatgc caaactcgtt attcggttag atacaatgct tcagggagca attctttgcc
      481 atatcactgc ctgaccgccg tggtgctatg gacgtccaag gtgccctcga tcatttctcg
      541 gataatgacg ccaagcctat aaccagttct caaaggcgat ccgagtgagg ttggcaaagg
      601 aatcggcgat ccgtcagccc ttggcgtgac ccgcgctgga aaatccattg ccggaatggg
      661 aaatcaaact acgcacggcg atgaaacatc tccgttttcc gccagggcag tgggcttgcc
      721 cagatattcc cgtcggcccg gctaaggatc ctcgaatgag cagccaggaa tatcggcgcg
      781 tttgccagaa gtggagaacc gcccggccaa cggcgttttt cagtccttgc ttgcgcttcc
      841 gaggggtttt ggatagaaat agagcattgt ttccactggg agctgattgc cccggtgaat
      901 cggtcgtacg gctctatgtt tggctgcaaa aacctggcta tgaataaact tcgtttcgcg
      961 cctgcggtgt ttccgcttct ggtcggatgt ggtttggttg gcgcccttgc gatggccacc
     1021 cctctcgatg gaggcgaggt taacctgggc aatctctatc gcgtctccaa ggcggagtcg
     1081 cgctcaatca gtccggagaa ctttaccggc gagaagggca aggcgggcat ggcgaccgag
     1141 gggaccggca agggcccggc gcgggatttg gggcagggct ggaaggtttc gccttcggtt
     1201 cgtattaagg ccgggacgac ttttaccctg ggcgagatca acgggccggg cctcatccag
     1261 cagatttgga tgacgcccac gggcaactgg cgccgctcga ttctccgctt ttactgggat
     1321 gacgaggcgg agccttccgt cgagtgtccg gtgggcgact ttttcgcctg cggctggggc
     1381 aagtattgcc aggtcagttc gctggcggtc tgcgtcaacc ccggcagtgc cttcaactgt
     1441 tattggccga tgccgttccg caagaaagcc cggatcacga tggagaaccg ggacgcgcag
     1501 gatatgatcc tttattacca gatcaattat gccctcgcgc cggtgccgaa gtcggcggcg
     1561 tatttccacg cgcagtttcg ccgtgaatct ccgttaaaac agaagggcct ctataccatc
     1621 ctcgacggcg tcgccggcca gggacattac gtgggcacct acctggcgtg ggaggtgcac
     1681 agtcccggtt ggtggggcga gggcgaaatc aagtttttta tggacggcga tcggcagttc
     1741 ccgaccatct gcgggaccgg cacggaggat tatttctgcg gttcctacaa cttcgagaat
     1801 cgcgagacga agaagtacca ggtgttttgc acgccttacg ccgggctggc gcaagtcctg
     1861 ccgccggacc ggatttacga ggtgggccag cgatttggcc tgtaccgatg gcatatcccc
     1921 gacccggtgc gcttcaaaaa ggacctcaag gtcacgatcc aggctttggg ctggcaggag
     1981 ggcgggcggt atctgccgct ggaggatgac attgcctccg tcgcctattg gtaccagaag
     2041 gagccgcacc aggcctttcc gaagctgcca cctaatctct aagcccaatt cccggcccat
     2101 gttcgccccc gctcccgccc gaccgcgccc gaccgccgcc cgacgagatc tcctagcggt
     2161 ttccgcggct ggggacggcg cgacgctggc tgccagcggt gcccaaccgg gatggggcgg
     2221 ctgctatttc gggccgctgc cggtttcggc cggattgacg ccacggccgt tggccggaag
     2281 tttttgagac ccctaacccc ttttaacgta aatatatgaa catcaccccc tcctgctcac
     2341 gacgcagttt tatcaaaact accggcctgg ccttgtctgc cgggatgctc tccaccgcca
     2401 gcccgctgct ggccgccgga aaaaagaaaa cactggttgg ctgtcgcgac gtgcatctga
     2461 aggtggccgg cctgccggat agctggtcca gcatgaaagc ggtgggcgcg gaagcgatcg
     2521 aggtctgcgt cgagttggac ctgacctgcc ccttgctgtt tcacccgcag cgcaagtaca
     2581 cgctggccac gccggaaggc attcgaacgc tcaaggatga cctttcggcc agcggttgcc
     2641 gtatcgcggc gtttatgatg ggcaaccgct ttgacgagcg cctggaggag gagcttgagt
     2701 gtgcgcgccg gttgattccg gccgcccagc agctcggcgt cgagatcatc cggattgatg
     2761 tcgtgccgcg caaactggag ggagaccagt tcctttcctt tgcgatcgac acttgcaaac
     2821 gtctctgcgc cctggcggag gactcgccca ttcgctacgg cgtcgaaaac cacggcaaga
     2881 ttactaatga cccgcaattc ctggaccggc ttttcgacgg cgtcggctcc tgccggcttg
     2941 gcctgacgct cgacaccgcc aacttctact ggtgggggca tccggtgagc gaactttatc
     3001 ccatttacga aaaatttgcc ccccgcgtcg tgcatacgca ttgcaagagc atcggctacc
     3061 cggacgataa gaaaaatgtg cgccgcgaga tgggctggga atacggcaaa tactgctgtc
     3121 cgatctacga aggcgacctg gattttgcgc gcatcgttaa aatcctgcgc aaagcccggt
     3181 atcgcggcga cctgtgcgtc gaaaacgagt cgctgggccg gtttcccgaa aaccagcgcg
     3241 ccgacatcct gcgccgggag attgccttgc tgaagaagct ggcctagcgc agcgcctttc
     3301 cccggcgctg gtcccggccc gcctggcgga gggacaaagc gcttgccccc gggcatgggg
     3361 tttggtttga tagccctgca tcatggcgaa tgcgatacaa gttccccggt cccatttgat
     3421 cctcgggctg tctctgcccc tggcggtgct ggtcgggtat tttttggccg agccgatgga
     3481 actcggcagc gcggcggtgg tgctggcggt cgtgggcgtg cttagcgttc ccctgctgat
     3541 gcggtggtat cacccggtgt tgatcttcct ctggaactcg gcgattaatc cggtcgtgct
     3601 gccggggcgg gcggggttgt ggatggtggt gacctgcatc gccctgttgt ttgctgtcct
     3661 gaaccgggcg gtaaacccga aggcacgttt cattatggtt cctgcggtga cctatcccct
     3721 gctggcattg ggggtattgg tggtaatcac cgcggtgttg cggggagggg ctggctttgc
     3781 cagcctgggc tcttcacagt atggcgggag ccgatatgcc aacctgctga tgggtattgc
     3841 gggttatttc gctttgacca gccgaaggat tccgccgtcg cgggccggac tttatgtggg
     3901 ctgctttttt ctttccgggc tgacctatgc catttccgac cttgccatcc tcgccgggcg
     3961 ccaattttac tttctccttt atatcttttc cacgacttat gcgacagatc aactcaagta
     4021 ccttgagaca ttcaatccaa gcatcattcg ctttcgtgga acttccgtag tggcaagcgc
     4081 tatttacact tacctgctgg cgcgctttgg cattcgcggt attttggatg tggcgcgccc
     4141 atggcgaggg ctgatttttc tgttggccgt tgcggggggc atgggcggcg gatttcgcag
     4201 ttatgtggcg ctctttggtt tgacttgtat cggcgtattt taccttgaag gacttcatcg
     4261 gactcgctac ctgccggccc tgctggcggt ggccctggtg ggaggagccc tggtcctgcc
     4321 ccaggcggat aaattgccgc tggccgtgca gcgaggattg gtttttctcc ccggcaaatt
     4381 tgattcgctc gccagacaaa gcgcgaaaac ctcgattgaa tggcgggtgg aaatgtggaa
     4441 acgtgtatgg cctgaagttc ctaagtattt atttctgggg aaaggttata agctggatcc
     4501 ctcggaactt tttttggctc agtatttctc gccccggtat catgatgcca tggacagcgg
     4561 gtcgcaggtt gctggcgatt atcacagcgg gccgctctcg gttctcgtcc ccttcggcat
     4621 tttcggaacg ctggcgtttc tttggttcct ggttgctggc acgcgcaccc tgtaccttta
     4681 ctacaagcat ggctcccctg tttatcaaac agtaaacacg ctattactgg cggcctttgc
     4741 ggctcgggcc atattctact ttgtcggctt tggatccttg cacacggaca tggctttctt
     4801 caccggcctg ctcggcctcg gcgtcgctct gaatggcacc ccttctgctg ctgctgcatc
     4861 gtcaccatct gaacaaccgg atactggcgt ggagctcaat acggagtatg ttaaggtgta
     4921 gcgctgaacg ggtttaattc ataatgtcta acgcatcggc actttttcgg tccctgatcg
     4981 tttatgggct ttgcctgccg ctggccgtgg cattggggta tttgttggcg aatccgctgg
     5041 atctgaccac ggttgtggtg gtgggatccc tgtgtttcat tctgatgatt cccatctttt
     5101 tgcgctggca tcatgtctgg ctcattgccg cctggaacat gtcagcggtg gtgttctttc
     5161 tgcccggtcg gccgcc
//
LOCUS       DAOPQG010000162         5147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_17622_length_5147_cov_14.458955, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000162 DAOPQG010000000
VERSION     DAOPQG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17622_length_5147_cov_14.458955"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKI07_13165"
     CDS             complement(<1..544)
                     /locus_tag="PKI07_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOC52032.1"
                     /translation="MDSTFSSFERTEAPGATPGPTALLRFGPSAIHGQGGFARTRIAR
                     GTRVVEYVGERISKSESLRRCEANNAYIFTLNDEQDLDGNVARNPARLLNHSCAPNCE
                     AELCEDRIWIVARRDIAAGEEITFNYGYDLVDYPEYPCRCGSPDCVGYIVAEEFFAHV
                     RQRRAAGAEAASDRPDGGPGF"
     gene            complement(519..1358)
                     /locus_tag="PKI07_13170"
     CDS             complement(519..1358)
                     /locus_tag="PKI07_13170"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOC52033.1"
                     /translation="MAKPGHIELPTGERIPILYEDRSVLAIDKPPGWMLVPFSWQKTN
                     RNLQAALVSSIASGAYWARARGLKYLRFVHRLDAETSGVLLLAKSPGAVHSLGALFEG
                     RRVAKVYLAVVSGVPGSEAWTCRQKLAPDPGVIGRMRAVPRDGKEAETHFRVLQCRGQ
                     RALIEARPVTGRTHQIRVHLAASGHPVEGDPLYGTPRAKSEKPSRRPARRDAGAGAAA
                     PAGGEADGVRAPADGARGPGTGMGLRAVRLGYTDPFTRRRVDIQAPWEPFVSEYGFDV
                     FQF"
     gene            1498..2010
                     /locus_tag="PKI07_13175"
     CDS             1498..2010
                     /locus_tag="PKI07_13175"
                     /EC_number="3.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HOC52034.1"
                     /translation="MLNPLAMKPEPKPGEWLAPDDFDTVVRLTPLVAIDLLVQAPDGR
                     VLLGRRVNQPAKGSFFVPGGRISKNETLAAALRRISRAELGVEVRLEDTRFRGVYEHF
                     YATNSRQTPGFGTHYVVLAYGLTAAPETLNLPKDQHGEFLWLSEADLLSNPEVHLNTK
                     NYFAPAAKTG"
     gene            2540..4282
                     /locus_tag="PKI07_13180"
     CDS             2540..4282
                     /locus_tag="PKI07_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC52035.1"
                     /translation="MTKLARLPRRSFLKGLGLAAPAVLTGPRLAVAQADAPAPAAAPA
                     HPPGAKELRADVVIIGGGLGGCAAALAAARNGLRVILTEETDWIGGQLTAQAVPPDEN
                     PWIETFGGTRSYLHLRQQIREYYLRHYPLTAEARARPDLNPGNGWVSRLCHEPRVALA
                     VLHQLLAPYVSGRKVLLLLRHQAVDALTAGDRVEAVRARSLESGNDTVLRAPYFVDAT
                     ELGDLLPLTRTEYVTGAEAQADTGEPHAPAVAAPLNMQAFTCCFAMDYVAGEDHTLEP
                     PREYAFWRDYVPALRPPWPGRLLAWEYSHPASLKPFKLETDPAKGTGLWAYRRIADRS
                     QFTAGAYPGDLTLVNWPQNDYLLGNLCEVSAAEAARHVARAKQLSLSLLYWLQTEAPR
                     PDGGTGWKGLRLRPDVVGTEDGLARHPYIRESRRIRAEFTVVEQHVATEFRMQETRAS
                     REEVKAAEFKDSVGIGCYRIDLHPSTGGDNSIDISSLPFQIPLGALLPRRVENLLPAA
                     KNLGVTHITNGCYRLHPVEWNLGEAVGALVAFCTTRKRLPRQVRQQADLLRDYQKLLA
                     DQGVPLAWPKVGPR"
     gene            4358..4684
                     /gene="cutA"
                     /locus_tag="PKI07_13185"
     CDS             4358..4684
                     /gene="cutA"
                     /locus_tag="PKI07_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOC52036.1"
                     /translation="MKPRNTFIMVLVTAPDLPTARKLARGALAARLIACANLVPRLES
                     HYWWEGKITAGREVLLLMKTTAARRAALEKWIVARHPYDTPEVVALPIAHGHRRYLDW
                     VRDAVQ"
     gene            4681..4950
                     /locus_tag="PKI07_13190"
     CDS             4681..4950
                     /locus_tag="PKI07_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC52037.1"
                     /translation="MTPPETCPHCGADVPPAARACPECGADERTGWSEAARADGLDLP
                     DDAFDYEDFVRREFGGRRAVPRGLHWFWWVAALAALAAFLAHWVW"
BASE COUNT          842 a         1849 c         1659 g          797 t
ORIGIN      
        1 aaaaacccgg accaccgtcc ggccggtcgg aagccgcttc cgcccccgcg gcgcgccgct
       61 ggcgcacatg cgcaaaaaac tcctcggcta cgatgtagcc cacgcagtcc ggcgaaccgc
      121 agcggcaggg atactccggg taatccacca ggtcgtagcc atagttgaag gtgatctcct
      181 cccccgccgc aatatcgcgc cgggcgacaa tccagatgcg atcctcgcac agctccgcct
      241 cgcagttggg agcgcaactg tggttgagca gccgcgccgg gttccgcgcc acattcccgt
      301 ccaggtcctg ctcgtcgttg agcgtgaaga tatacgcgtt gttcgcctcg cagcggcgca
      361 gcgactccga cttgctgatc cgctccccca catactccac cacgcgcgtg ccgcgcgcga
      421 tccgcgtccg ggcaaacccg ccctgcccgt gaatcgccga cggcccaaac cgcagcagcg
      481 ccgtggggcc gggcgtggcc cccggcgctt ccgtccgttc aaaactggaa aacgtcgaat
      541 ccatactcgc tcacaaatgg ctcccacgga gcctgaatat ccacccggcg gcgggtgaat
      601 gggtccgtgt aacccagccg caccgcgcgc aaacccattc ccgttcccgg tccccgcgcc
      661 ccgtccgcgg gcgcccggac gccatccgcc tccccgcccg ccggggccgc ggcaccggcg
      721 ccggcatcgc gccgcgcagg ccgccgggac ggtttttccg acttcgcgcg cggcgttcca
      781 taaagcggat ccccctccac cggatgcccg gaggcggcca ggtgcacgcg gatttggtgc
      841 gtccttcccg tcaccggccg cgcctcgatc aaggcccgct gaccccggca ttggagcacg
      901 cgaaagtgcg tttccgcctc cttgccgtcc cgcggcacgg cccgcatccg cccgatcacc
      961 cccggatccg gcgccagctt ctgccggcac gtccaggctt ccgaccccgg caccccggac
     1021 accaccgcca ggtaaacctt cgccacccgg cgcccctcga aaagcgctcc cagagaatga
     1081 accgcccccg ggcttttggc cagcagcaat accccgctgg tctccgcatc cagccgatgc
     1141 acaaaccgca aatacttcag cccccgcgcg cgcgcccaat acgcccccga cgcgatggac
     1201 gacaccagcg ccgcctgcag gttccggttc gtcttctgcc acgaaaacgg caccagcatc
     1261 cacccgggcg gcttgtcaat cgccagcacc gagcggtcct catataaaat gggaatccgc
     1321 tcgcccgtcg gcaattctat atgtcccggc ttggccatac gcgggcgcca ggcgcccctg
     1381 aatcattcgg ttccccggca ccatgcctga aaaacacctc ccggaaaaag caaaatatcc
     1441 gcccgcgccg aacccgccgc ccggttttat ccttgtgctc gcggcccccg aacgcctatg
     1501 cttaacccgc tcgcgatgaa gcctgaaccc aaacccggcg agtggctcgc cccggacgac
     1561 ttcgacaccg ttgtccggct gacccccctg gtcgccattg acctgctcgt gcaggcgccc
     1621 gacgggcggg ttctgctggg ccgccgcgtc aaccagccgg ccaaaggctc cttctttgtc
     1681 ccgggcggcc ggatttccaa aaacgaaacc ctggccgccg ccctccggcg catatcccgg
     1741 gccgagctcg gggtggaagt ccggctcgaa gacacccgct tccggggggt gtatgagcat
     1801 ttctacgcca ccaacagccg ccaaaccccc ggcttcggca cccattacgt cgtgctggcc
     1861 tacggcctga ccgccgctcc ggagaccctg aacctgccca aagaccagca cggcgagttc
     1921 ctctggctct ccgaagcgga cctgctaagc aacccggagg tccacctgaa tacaaaaaac
     1981 tacttcgccc ccgccgctaa aaccggctga aacatccccc tccccccgtc tcctaccccc
     2041 gatttctccc tctgccccgc tgctccagca gcgaaaacca ccctaaaaac cgcccccaaa
     2101 accccctcgg gcttccagct ccctcaaaaa acctcaactc actgcaatca cgccacttcc
     2161 accctgccca ctggccgtgt acaaggggtg tacaagggat gctcatcggg agtacaacct
     2221 aaaatctgtc tgacatccgg tgggcatccc ttgtacaccc cttgtacacg accagtggat
     2281 agggtggcga gagcctgaaa ttgggcgggc gggccggggg cgagggcgac gttggcggag
     2341 ggctgtggat ggatggtgat tggggacgtg gggagagtgt ccaataattg gacagtcgag
     2401 gatggggtcg gaaggtgagg ttccgggatg gggtatggtc gagatggagg aggtttccgg
     2461 cgtattctgg aaagcgggca tgggtgggtg gatttgcgcg ttggcttgtc aggcgcgtgg
     2521 gggatggtag catgggggca tgactaagct ggctcgatta cctcggcgct cgtttctcaa
     2581 gggtttgggc cttgcggccc cggcggtctt gaccgggccg cggcttgccg tcgcgcaggc
     2641 cgacgccccg gcgccggcgg cggccccggc gcatcctccc ggggccaagg aattgcgggc
     2701 ggacgtggtc atcatcggcg gcggactggg cggttgcgcg gcggcgctgg cggcggcgcg
     2761 caacgggttg cgagtcatcc tgacggagga aacggattgg attggggggc agttgacggc
     2821 gcaagccgtg ccgccggatg agaatccgtg gatcgagacg ttcggcggca cccgcagtta
     2881 cctccacctg cgccagcaaa tccgcgagta ttatctgcgg cattacccgc tcaccgccga
     2941 ggcgcgggcc cggccggacc tcaaccccgg caacggctgg gtgtcccggc tctgccacga
     3001 gccgcgcgtg gccctggcgg tgctgcacca gcttctggcg ccgtatgtca gcggccgcaa
     3061 ggtgctcctt ttgctccgcc accaggccgt ggacgcgctc accgcgggcg accgggtcga
     3121 ggcggtccgg gcgcgcagcc ttgagtccgg caacgacacc gtcctgcgcg cgccgtattt
     3181 cgtggatgcg acggagctgg gggatctgct gccgctgacg cggacggaat acgtcaccgg
     3241 ggccgaagcg caggcggaca ccggcgagcc gcacgcgccg gccgtggccg cgccgctgaa
     3301 catgcaggcg ttcacctgct gctttgcgat ggattacgtg gcgggcgagg accacaccct
     3361 cgagccgccg cgcgagtacg cgttctggcg cgactatgtg cccgccctcc ggccgccctg
     3421 gcccgggcgg ctgctggcct gggagtacag ccatccggcc agcctgaagc cgttcaaact
     3481 ggaaaccgat cccgcgaaag gcacggggtt gtgggcctac cggcgcatcg ccgaccggtc
     3541 gcagttcacc gccggcgcgt atccgggcga cctgacgctg gtgaactggc cgcaaaacga
     3601 ttacctgctc ggcaacctct gcgaggtcag cgccgcggaa gccgcccggc atgtggcccg
     3661 ggccaaacaa ctgagcctgt cgctgctcta ctggctgcaa accgaggccc cccgccccga
     3721 cggcggcacc ggctggaagg ggctgcggct gcggcccgac gtggtgggca cggaggacgg
     3781 cctggcccgc cacccctaca tccgcgagag ccgccgcatc cgcgcggagt tcacggtcgt
     3841 cgagcagcac gtcgccaccg agttccgcat gcaggaaacc cgcgcctcgc gggaggaggt
     3901 caaggcggcg gagttcaagg attcagtggg cattggctgc taccggattg atctccaccc
     3961 cagcacgggg ggcgataact cgattgatat cagctccctg ccgtttcaga tccccctggg
     4021 cgccctgctg ccccgccgcg tcgaaaacct cctccccgcc gccaagaacc tgggcgtcac
     4081 tcatatcacc aacggctgct accggctgca cccggtggaa tggaacctgg gcgaggcggt
     4141 gggcgcgctg gtggcgtttt gcacgacccg caaacgcctc ccccgccagg tgcgccagca
     4201 agccgacctg ctgcgggatt accagaaact gctggcggac cagggcgtgc cgctggcgtg
     4261 gcccaaagtc gggccgcgct gaagaacccc gcccgccccg gggacgcccg cggcgccgcc
     4321 gccccccggc cgggcaatgc tcccgatgaa atcacacatg aagcctcgca atacgtttat
     4381 catggtgctg gtgacggcgc cggacctgcc gaccgcgcgg aaactggcgc ggggggcgct
     4441 ggcggcgcgg ctgattgcct gcgccaacct cgtcccgcgc ctcgaatcgc attactggtg
     4501 ggagggaaag atcacggcgg gccgggaagt gctgctgctg atgaaaacca ccgcggcccg
     4561 gcgggcggcg ctggaaaagt ggatcgtggc ccggcatccc tatgacacgc cggaggtggt
     4621 ggcgctgccc atcgcccacg gccaccgccg ctacctggac tgggtgcggg acgccgtgca
     4681 atgaccccgc ccgagacctg cccccattgc ggcgccgacg ttccccccgc ggcccgggcc
     4741 tgcccggaat gcggggcgga tgagcggacc ggctggtccg aggcggcgcg cgcggatggc
     4801 ctggacctgc ccgatgacgc cttcgactac gaagacttcg tccggcgcga gttcggcggc
     4861 cggcgcgccg tgccgcgcgg ccttcattgg ttttggtggg tggcggcgct ggccgcgctg
     4921 gcggcgtttc tggcgcactg ggtctggtag ccggcggaaa atgtggtttt gcggttgcgg
     4981 cgcggcgggg gattgttcta accgggatat ttcacgcccc gcacgaaata cccgcaggcc
     5041 ggagcgcggg cctctggccc gcttagcccg tttggaggct gtaagcgggc aagatgcccg
     5101 cgctccagtc ttgcaggtgt gaaatgcctg ggctggagcc gatcact
//
LOCUS       DAOPQG010000163         4556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_20629_length_4556_cov_14.278383, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000163 DAOPQG010000000
VERSION     DAOPQG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20629_length_4556_cov_14.278383"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1091)
                     /locus_tag="PKI07_13195"
     CDS             complement(78..1091)
                     /locus_tag="PKI07_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC52038.1"
                     /translation="MTRRERVLAHLAGQPVDRLPLMPITMMFAGSLIGARYRDYCTDY
                     RVLVEGQIRTAEAFGFDYVNTMSDPAREAADCGAAVEYFDHQPVALVEGQSLLADKAR
                     LLSLKVPDPRGGGRMHNALKALELYQQRVGKEMIIEGWVEGPIAEAADLRGINTLMMD
                     FYDDPAFVRDLFAFVIEMELRFAREQIKAGADIIGVGDAAASLVGPGIYHEFVWPYEK
                     QLVDGIHALGGRVRLHICGNTRQILEGMGRLGCDIIDLDSLAPLAEARQKMGPDAVLL
                     GNLNPVTILRNGNPDDVTRAIAECHRQAGPRFIVGAGCEVPRDTPHENLRALCAYAHS
                     HRP"
     gene            complement(1098..1973)
                     /locus_tag="PKI07_13200"
     CDS             complement(1098..1973)
                     /locus_tag="PKI07_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOC52039.1"
                     /translation="MHPLLEELLSQGPVITDGAWGTELQARGLGAGETPDLWNLTHPE
                     RVGEVAAAYVAAGSRVILTNTFGANRIRLAEAAAGDQAGEINARGVEISRQTAAGRAR
                     VFASIGPTGKLLMNGDVTEAELWAAFREQARALAGAGADALVVETMSDLAEATVAIGA
                     ARETGLPVVASMVFDSGKEKDRTMMGNTPEQAVQALITAGADVVGANCGQGAAGFIGI
                     CRRMRAVTDRPIWMKPNAGQPIMVAGQPRYTTTPEEFAGYIPALVQAGANLVGGCCGT
                     RPEFIVAAGRALRHR"
     gene            complement(2002..2640)
                     /locus_tag="PKI07_13205"
     CDS             complement(2002..2640)
                     /locus_tag="PKI07_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOC52040.1"
                     /translation="MPDLKQLYDAVVSGDAKTAQLITKEALAAGIDPLKLVNEHMVPA
                     MDEVGRRFEANEYFVPELLISARAMKGALELIRPLLIARGDKPVGRVAIGTVKGDLHD
                     IGKNLVASLLEGGGFEVLDLGVNVSPEKFIEAVNAKQADIVAMSALLTTTMPAMKTTI
                     DALTQAGVRGKVKVLIGGAPITQKYADEIGADGYSENAVGAVTLAKKAVGVA"
     gene            complement(2707..3372)
                     /gene="folK"
                     /locus_tag="PKI07_13210"
     CDS             complement(2707..3372)
                     /gene="folK"
                     /locus_tag="PKI07_13210"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOC52041.1"
                     /translation="MKAKRQSPARPRRPDARKETADLGEWAIVALGSNLGDSRQNVLR
                     AMACLRQFSRHPLRKSSLWQTEPVDCPPGSPAFVNAVVAFAPRRGATPEALLTRLRAL
                     ERAFGRRPKKVMNEPRPLDLDLIAFGREIRASQRLTLPHPRAHLRRFVLQPLSEIAPD
                     LVLPGQAKPVWQLLTELPLDAAPGPEALPVRITQPGGRRAPAAFATCARRAGRTPKSA
                     IRR"
     gene            complement(3369..4103)
                     /gene="dapB"
                     /locus_tag="PKI07_13215"
     CDS             complement(3369..4103)
                     /gene="dapB"
                     /locus_tag="PKI07_13215"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOC52042.1"
                     /translation="MTKIIITGSKGRMGQALAACAAHHSDLQIVGLIDQGDDLRSVLD
                     RGEVVIDFSLHSASPGIAALCAAHRKALVVGTTGHSDLEKSQITGCQARIPVVLSSNF
                     STGINTLFWLTRKAAEILGAGFDLEIVEMHHRLKRDAPSGTARTLAEILAEVRQQQLG
                     QVLRHGRAGIVGERPAEEIGLHSVRGGDVVGDHTVIFAGAGERVELTHKASTRETFAE
                     GALRAAVWAVKQPPGLYDMQHVLGLK"
BASE COUNT          817 a         1523 c         1437 g          779 t
ORIGIN      
        1 atcaaatcct agaaaaagtg ggtcactgca aagctaagac tgttacacca cactaacgtt
       61 cgtcgggaat cccgcggcta cgggcgatgg gagtgcgcgt aggcgcaaag ggcgcggaga
      121 ttttcgtggg gagtatcacg gggaacttca caaccggcgc cgacgatgaa tcgcggcccg
      181 gcctggcggt ggcattccgc tatggcccgg gtgacgtcgt ccggattccc attgcgcaga
      241 atggtgaccg ggttcaggtt gccaagcagg acggcgtccg gccccatctt ttgccgcgcc
      301 tcggcgagcg gggctaggga gtccaggtca ataatatcac agccgagccg gcccatgccc
      361 tccagtatct ggcgggtatt gccacagata tgcaggcgga cccggccgcc aagggcgtgg
      421 atgccgtcca caagctgctt ttcgtaaggc cagacaaact cgtggtaaat ccccgggccg
      481 acgagcgacg cggcggcatc gccaacgccg atgatgtccg ccccggcctt gatctgctcg
      541 cgcgcaaacc gcagctccat ttcaataacg aaagcgaaga ggtcgcgcac aaaagccggg
      601 tcatcgtaga agtccatcat cagggtgtta atgccgcgca ggtcggcggc ttcggcgatg
      661 ggcccctcga cccagccctc aatgatcatt tccttcccga cgcgctgctg gtacaactcc
      721 agcgccttga gggcgttgtg catacggccg ccgccgcgcg ggtccggaac tttcagggag
      781 agcagccggg ccttatccgc cagcagggac tggccttcca cgagcgcgac gggctgatga
      841 tcgaaatact ccaccgccgc gccgcaatcc gccgcctcgc gtgcgggatc ggacatggtg
      901 ttgacgtagt cgaagccgaa ggcctccgcg gtgcgaattt gcccctcgac cagcacccgg
      961 tagtcggtgc agtaatcacg gtagcgcgcg ccgatcagcg agccggcgaa catcatggtg
     1021 atgggcatga gcggcagccg gtcaaccggc tggccggcca agtgcgccag aactcgctcc
     1081 cggcgggtca tcggcggtca gcgatggcgc agggcgcgcc cggcggcgac aataaactcg
     1141 ggccgcgtgc cgcagcaccc cccgaccagg ttggcacccg cctgaaccaa ggccggaata
     1201 taaccggcaa actcctcggg agtcgtagtg tagcggggtt gcccggccac cataatcggc
     1261 tgcccggcat tgggcttcat ccaaatgggc cggtcagtga ccgcgcgcat tcgccggcag
     1321 atcccgatga atccggcggc cccctgaccg cagttggcgc cgacgacatc cgcgcccgcc
     1381 gtgatcagcg cctggacggc ttgctcgggc gtatttccca tcatggtgcg atccttttct
     1441 ttgccgctgt cgaagaccat gctggccacg accggcaggc ccgtctcgcg cgcggcgccg
     1501 atggccaccg tggcctccgc caggtcggac atcgtttcga caaccagcgc gtcggccccg
     1561 gcgccggcca gggcgcgggc ctgctcgcgg aaggccgccc acagctcggc ttcggtgaca
     1621 tcgccgttca tgagcagttt gccggtcggg ccgatcgagg cgaacacgcg ggcgcggccg
     1681 gcggcggtct ggcgcgaaat ctccacgccg cgcgcgttga tctcgccggc ttgatctccg
     1741 gcagccgctt cggcaaggcg gatgcggttg gcgccgaagg tattggtgag aatcacccgg
     1801 ctgccggcgg ccacatacgc cgcggcaact tcgccgacgc gctccgggtg ggtgaggttc
     1861 caaaggtccg gcgtctcccc cgcgcccagg ccccgcgcct ggagttcggt gccccatgcg
     1921 ccgtcggtta tgaccggccc ctgggaaagg agttcctcaa gaagcggatg catggccagc
     1981 ggagaaaccc gtgccgcggg atcaggctac ccccacggcc ttcttggcca gagtcacggc
     2041 gcccaccgcg ttctcgctgt agccatccgc gccgatttcg tcggcgtatt tctgggtaat
     2101 gggcgcgccg ccgatgagaa ccttgacctt gccgcgcacg cccgcctggg tgagcgcgtc
     2161 aatcgtggtc ttcatggcgg gcatggtcgt ggtcagcagg gcggacatgg ccacaatgtc
     2221 ggcctgcttg gcattgaccg cttcaataaa cttctccggg gaaacattga cgcccaggtc
     2281 gagaacctca aaaccgccgc cctcgagcag ggaagcgacc aggttcttgc cgatatcgtg
     2341 caaatcgcct ttgacggtgc cgatggcgac gcgcccaacc ggcttgtcgc cgcgcgcgat
     2401 caggagcggc cggatgagct cgagggcgcc cttcatggca cgcgcggaga tgagcaactc
     2461 gggcacgaaa tactcattcg cctcgaaacg gcgtcccacc tcgtccatgg cgggcaccat
     2521 gtgttcgttg accagcttca gcgggtcaat cccggcggcc agggcttctt tggtgattag
     2581 ttgggcggtc ttggcgtcgc cgcttaccac ggcatcgtag agttgtttta gatccggcat
     2641 gaagttcctt ttaatacgat ttgcacacgt tgttgcggcc acactaggcg gggaagatat
     2701 ttcgagtcaa cgccgaattg cagattttgg cgtccgcccc gcacgcctgg cgcaagtggc
     2761 aaaggcggcc ggcgctcttc gcccgccggg ctgggttatc cggaccggca gggcctccgg
     2821 gccgggcgca gcgtccaggg gcaattccgt cagcaactgc cacaccggct tcgcctggcc
     2881 gggaagcacc aggtccggcg caatttcgct caacggctgg agcacaaagc ggcgcaggtg
     2941 ggcgcgcgga tgaggtagcg tcagccgctg gctggcgcgg atttcgcggc caaacgcaat
     3001 caaatccagg tcgagcgggc gcggttcgtt catcactttc tttggccttc ggccaaaggc
     3061 gcgctccagc gcgcgcaggc gcgtcagcaa cgcttccggg gttgcccccc gccgcggcgc
     3121 aaaagccacg accgcgttca cgaacgccgg cgagcccgga ggacaatcta ccggttccgt
     3181 ttgccacaag gaggacttgc gcaagggatg ccgggagaat tgccgcaagc aggccatcgc
     3241 ccgcagcaca ttctgccgcg aatcccccag gtttgagccc agggccacga tggcccactc
     3301 cccgaggtcc gctgtttctt tccgcgcgtc cggacggcgg gggcgggcgg ggctttgtct
     3361 tttcgccttc atttaagccc cagcacgtgc tgcatatcgt agagcccggg cggttgcttc
     3421 accgcccaca ccgccgcccg cagcgcgccc tcggcaaacg tctcccgcgt ggaagcctta
     3481 tgggtcagtt caacgcgctc gcccgcgccg gcaaaaatga ccgtatggtc ccccaccacg
     3541 tcgccgccgc gcacggagtg caggccgatc tcctccgccg gacgctcgcc cacgatgccg
     3601 gcgcgtccat gccgcaggac ctggccaagc tgctgctggc ggacctccgc cagaatctcc
     3661 gccagcgtcc gcgccgtccc gctgggcgca tcgcgcttca ggcggtggtg catttcaaca
     3721 atctccaaat caaagcccgc tcccagtatc tccgccgcct tgcgcgtcag ccagaacagg
     3781 gtgttgatgc ccgtggaaaa gttcgaggac agcaccacgg ggattcgcgc ctggcaaccg
     3841 gtgatctgcg atttctccag atccgaatgg ccggtagtgc cgacgaccag cgccttccga
     3901 tgcgcggcac acagggccgc aatgcccggg gaggcactgt gcaggctaaa atcaatgacc
     3961 acctcccccc ggtccaacac cgaacgcagg tcgtcgccct ggtcaatcag gccgacaatt
     4021 tgcaaatccg aatgatgcgc ggcgcaggcg gccagcgcct ggcccatgcg gcctttcgag
     4081 cccgtgatga tgatcttcgt catacgtggc gcgccgcgcg gggcgcgtcc gcccacggac
     4141 cacgcggcgc acggcgcttt gccgtcccaa tacgcccgtc gccgggggaa aagtcaaacg
     4201 gataagcaat agccctttac gcccccaagc gccccggata cggcaaggat ggtgggaaat
     4261 tggcctggag gctcgtccgg caggtgccgc gatagccaga acccgcccag gcgcaggctc
     4321 gcatttttgc ggcggcagaa atcccccgat gttttgctca atagcccatg ttcggaaccg
     4381 gaataatgcc agacgccgca actcaccacc cgtcgcagaa ctgtccaatt attggacact
     4441 aagctgtttt cgcccctgca aatccttccc aaggccactt tgaatttaac ccgcgtaatt
     4501 tcaggcggta gtgtcctaaa agttctgcaa aaagcagcgg gtcatggtaa gccact
//
LOCUS       DAOPQG010000164         4499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_20963_length_4499_cov_13.694194, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000164 DAOPQG010000000
VERSION     DAOPQG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20963_length_4499_cov_13.694194"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            561..636
                     /locus_tag="PKI07_13220"
     tRNA            561..636
                     /locus_tag="PKI07_13220"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:594..596,aa:Ala,seq:ggc)
     gene            complement(683..2113)
                     /locus_tag="PKI07_13225"
     CDS             complement(683..2113)
                     /locus_tag="PKI07_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC52043.1"
                     /translation="MTRNTKVLLIEDDRSIATGLQKAMRGSGYDVAVQTRGDTGLRHA
                     LAEAYDIVITDLRLPGTDGLELVRQLHQAKPKLPIILITAHGSTETAIEATRWGAFDY
                     LPKPFDVEELLELVGKALDSSRLMSEPIEMGDAVSARTAIVGNSRVMQSIYKEIGRVA
                     ATPVTVLIRGDTGTGKELIARAIYQHSNRAAAPFIAINCAAIPETLLESELFGHERGA
                     FTGADTRRIGRFEQAHGGTIFLDEIGDMSVRLQAKLLRVLQEKTIQRVGGKEPVPVDV
                     RIIAATHRDLEAAIRERLFREDLFYRLSVVTLSLPPLAERAEDIPDMVRYFLRRYAPE
                     IGVASPAISPEAIAYLQSQPWPGNVRELENATRKALLLARDYTIGVDLLREVVAKTRQ
                     PLAASQQTHAAYITELMDAVERGETHNAFARMLADLEPELYAQAIRRAQGNLTRAAQW
                     LGVTRLKMREKLKEFGLHPRRETSGD"
     gene            complement(2097..3140)
                     /locus_tag="PKI07_13230"
     CDS             complement(2097..3140)
                     /locus_tag="PKI07_13230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC52044.1"
                     /translation="MSAKMPFRLTELVLAAVLAAAALVWVERTTWRQISALRAELNGA
                     ETARLLAADASAGERFRAEANAALTRLQRFLFLSLLGLVFGGAGVVVLIYRRLIVPLR
                     STLSESRAIIERQEKLASLGVFAAGIAHEIRNPLTAIKVRLFTLRNSHKPGTSEHEDL
                     AVIHNEINRLETIVQDFLQFARPAEPELQTLPVGRLLRGTGELLETGLAARGIRLKLE
                     LRTDEPVRVDPDKMKQVLINFVQNGADSMPSGGTVTLRSRQQRQVLNERPVNAVVIDI
                     ADAGTGMSSEVRSRLFDPFFTTKEQGTGLGLSIAARIVEKHGGIIQYETEPGQGTTFS
                     IVLPPAPPHDAEY"
     gene            3482..3952
                     /locus_tag="PKI07_13235"
                     /pseudo
     CDS             3482..3952
                     /locus_tag="PKI07_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
BASE COUNT          816 a         1544 c         1409 g          730 t
ORIGIN      
        1 ctactcgata agcgggcaat atgcccgcgc tccgtcaggc aattagcgca tccccgggcg
       61 gagccgtagc tcagttggac gctcggcaag gtctcgttca ccccggcagg ctggagttcc
      121 gcagcaaaca gccaaaagcg gaaagtgcaa aaggcaagag tgcccaagca tgcgcgaaac
      181 tgccgacggc gcgcgggcat cctgcccgct ttgcgggttg cctacccgat aagcgggcaa
      241 tatgcccgcg cgccgtcagg caattagtgc atccgggagg agccgtagct cagttcaatg
      301 ctcggcaagg actcgttcac cccggcaggc tgggtcccgc ggcaaccagc gcaaagcgaa
      361 aagtgcgcaa ggacaagagc gcccgagcat acgcgaaact gtcggcggcg cgcgggcatc
      421 ctgcccgctt tgcgggttgc tcacccggta agcgggcaaa atgcccgcgc gccgtcaggc
      481 ctcgcagtca gcgcgcaaaa agaatgcaaa acagctttga caaatccggg ccgcccccta
      541 cattagtgtc atccccgggt ggggtcgtag ctcagttggc agagcgcctg aatggcattc
      601 aggaggtcag gggttcgaac cccctcggct ccaccatccc tcttcccgcc ccggccgccg
      661 ccgctttcag cggggcaacc ctttagtcgc cgctggtttc ccgccgggga tgaagcccga
      721 attccttgag tttctcccgc attttcaggc gggtgacgcc gagccattgc gccgcccggg
      781 tcagattgcc ctgggcgcgg cgaatggcct gggcataaag ctcgggctcg aggtccgcca
      841 gcatccgggc aaaggcattg tgcgtctcgc cccgctcgac ggcgtccatc agttccgtga
      901 tataggcggc gtgcgtttgc tgggaggcgg ccagcggctg gcgggttttg gcgacgacct
      961 cgcgcaggag atccacgcca atggtgtagt cccgcgccag cagcagggcc ttgcgcgtgg
     1021 cattttccag ctcgcggaca ttgccgggcc agggctggct ctgcaggtag gcaatggcct
     1081 ccggcgaaat ggccggcgag gccacgccga tttccggcgc gtagcggcgg agaaaatagc
     1141 gcaccatatc ggggatgtct tccgcgcgct cggccagcgg cgggagggag agggtcacga
     1201 cgctgaggcg gtaaaaaagg tcctcgcgga acaggcgttc gcggatggcc gcctcgaggt
     1261 cgcggtgggt ggcggcgatg atgcggacat ccaccggcac gggttccttg ccgcccaccc
     1321 gctgaatcgt cttttcctgc agcacgcgca gcagcttggc ctggaggcgg acgctcatgt
     1381 cgccgatttc atccaggaag atggtgccgc cgtgcgcctg ctcgaagcgg ccaatgcgcc
     1441 gggtgtcggc accggtaaac gccccgcgtt cgtggccgaa cagctcgctt tccaggagcg
     1501 tttcgggaat ggcggcgcag ttgatggcga taaacggcgc cgccgcgcgg ttgctgtgct
     1561 ggtaaatggc gcgggcgata agctccttcc cggtgccggt gtcgccccgg atgaggacgg
     1621 tgacgggagt ggccgccacg cgcccgattt ccttgtaaat ggactgcatg acccggctgt
     1681 tgccgacgat ggcggtgcgg gcggagacgg catcgcccat ctcgatgggt tccgacatca
     1741 ggcggctgct gtccagggcc ttgccgacca gctcgagcag ttcctcaacg tcaaacggtt
     1801 tgggcaggta atcaaaggcg ccccagcggg tggcttcgat ggccgtttcc gtcgagccgt
     1861 gggcagtgat gaggatgatc ggaagtttcg gcttggcctg gtgaagctgg cggacaagct
     1921 ccagcccatc ggtgccgggg agccgcaaat ccgtgatgac gatgtcatac gcctcggcca
     1981 gggcgtggcg cagtccggta tcgccgcggg tctgcacggc gacgtcgtag ccgctgccgc
     2041 gcatggcctt ctggaggccg gtggcgatgc tgcgatcatc ttcgatcagg agaactttag
     2101 tattccgcgt catggggagg cgccggcggg agcacgatgc tgaaggtggt tccctgcccc
     2161 ggctcggttt cgtattgaat gatgccgcca tgtttttcca cgatccgggc ggcgatggaa
     2221 agccccaggc ccgtgccctg ctctttggtg gtaaagaacg gatcgaacaa ccggctccgc
     2281 acttcggacg acatccccgt gccggcatcg gcaatgtcaa tgaccacggc gttgacgggc
     2341 cgctcgttga gaacctgccg ctgctggcgc gaccgcagag tcacggtgcc gccggacggc
     2401 atgctgtcgg cgccgttctg aacaaaattg atcaacacct gcttcatctt gtccgggtcc
     2461 acacgcaccg gctcatccgt gcgcagctcc agcttgagcc ggatgccccg cgcggccagg
     2521 ccggtctcca gcagctcgcc ggtcccccgc agcaaccggc ccaccggcag cgtctgcagc
     2581 tccggctcgg cggggcgggc gaactggaga aaatcctgca cgatggtctc gagccggtta
     2641 atctcgttat gaatcaccgc cagatcctcg tgctcggagg tgcccggctt atggctgttc
     2701 cggagggtaa agaggcggac cttgatggcg gtgaggggat tgcggatttc atgggcgatg
     2761 cccgcggcga aaacccccag ggacgccagc ttctcctggc gctcgatgat ggcgcggctc
     2821 tcgctcagcg tcgaacgcag cggcacaatc aggcggcgat aaatcaaaac aaccaccccc
     2881 gcaccgccga acaccagccc cagaagcgac aaaaagagga accgctgcaa gcgggtcagg
     2941 gccgcattgg cctcggcccg aaaacgctcc cccgccgaag cgtccgcggc cagcagccgg
     3001 gccgtttccg ccccgttcaa ctccgcccgc agagcactga tctgccgcca ggtggtccgc
     3061 tcgacccaca ccagcgcggc cgcggccagg acggcggcca gcaccagctc cgtaaggcga
     3121 aaaggcatct tagcactcat gggttcaggc cccgccagta taacggatgc agccccccgg
     3181 gcgaagcaaa aagcgccccc cgcccggccg gaggcggcgc gcccggcccg gcggcgggaa
     3241 tagttatgta aagcatccat gagcgccccc aagcacgccc ccggcctgga tgacggcacc
     3301 cgcccggggc gcgccctcgc gcagctatcc tcgccaccgc ctccatcaat gatgccatcg
     3361 agtattatga gctgttcaaa gcgcttcaag ccgccaagcg gcaggacgac cccgctttcc
     3421 agccgctcaa tatcgcttgc gtcttctctc cgcccgccca actggcggaa acgccggaga
     3481 gcgcatccgc accaagctgc tgaaggacgg ccatattgac accgttatcg gccttccccc
     3541 caacctcttc tactccaccg gcatccccgt ctgcatcctc gtcctcaagc ggtgcaagaa
     3601 acctgacgac gtcctcttca tcaacgccgc tgagcatttc gaaaaaggca agcgccagaa
     3661 tcgcctgctg gacgagcaca tcggcaaaat catcgagacc tacaaaaccc gcaagccgga
     3721 gccccgctac tcccgccggg tggagatgga cgaaatcgcg aagaacgaat tcaacctgaa
     3781 catctcccgc tacatcagca ccgctcagcc ggagccggag gttgacctcg ccgcgaccca
     3841 tgcccggctg cgggaaatcc aggacgccct cgaagccgcc accgccaggc acaaccgcta
     3901 cctggccgag ctcggcctgc ccccgctgcc ctccgccggc ggtggaaaat agccgccccg
     3961 aagcgcgtga ccccgttccc cgggccggcg ctgccccgcc cgcgtcaatc cgccaatccc
     4021 ggaacacggc tgcgacgccg tccgctcgat tccggccgtt ggctccgctc cagccggggc
     4081 gcggcggcgt ccggcccggc ggggcctttt atgagcgggg cctgagtttc ggtagctgag
     4141 gggaaacgat atttccagcc ccaaaccgct ttcgggttga gtgggcggga acgaaagcgc
     4201 ctatcaattc aaggtgaccg gccgaccctg agggcaacgc aagcagccgc cttgcggcag
     4261 cctccgcccg ggagggaaaa tctccccgcc agccgcgacc ccatcccgcc aaaaaaccac
     4321 ggcgcacgac aacacccggc cgcccgtcct ggctttgccg aacgttcgcg tcctctccct
     4381 cccgccttgg aacctcgttt tacatcatcc gcgactcgtc tatccctccc ttccggctca
     4441 ggcccgcgcc caaacccgtc ccacccgccc cagccagcct aacgctccca ttaggctgt
//
LOCUS       DAOPQG010000165         4486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_21056_length_4486_cov_12.895058, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000165 DAOPQG010000000
VERSION     DAOPQG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21056_length_4486_cov_12.895058"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..1221
                     /locus_tag="PKI07_13240"
     CDS             448..1221
                     /locus_tag="PKI07_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC52045.1"
                     /translation="MIALNGFRRRVCRLRAFTLVELLTVIALIAMLAALLLPAFSRAE
                     ARTREATCATNLRSWGQAFYLYAHDCQGYLPHPDDQGRNTPPFTFDPRHPEHECGYVD
                     VLPPMMGLRAWRDFPDGQKPAAGVWQCPAARPQPDSDYQRAFKPSSQGWHSYAMNSYL
                     AHEFPYGLPFGARPQPSFLKLERCTRPARTLLLFEQTLDPRPGYGQAGGLDTAGCYTA
                     EDPRALGERHPHRRAGLAGNVLYLDSHVGWREDLWNRAR"
     gene            1184..1759
                     /locus_tag="PKI07_13245"
     CDS             1184..1759
                     /locus_tag="PKI07_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52046.1"
                     /translation="MAGARTCGTAPADGATWLTADGVNPWASPGGDYDSAHPVAGVKG
                     GILDPDLHDRFFSWDITALLKDPATRAALQNCGALLKIDETPPPASGTPRAPFTSAYD
                     PGYPSSHWPTLQCTLAPVLDGVAIAGGAVSFAINGLIPGTANLVERSLDLAAADWTPV
                     ASFVAAGASTNWSEPLLPGGSQAFYRLRGPE"
     gene            1874..2395
                     /locus_tag="PKI07_13250"
     CDS             1874..2395
                     /locus_tag="PKI07_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52047.1"
                     /translation="MVALDFAAGQLAKSILQASGVGGFIRLDMLIPVTLWMLTRLVVD
                     RFGVLAAYQFAWGMLALFAFPGAILPGPLKLIPAVLQGLICDAGFSLLPRCPRARVLV
                     AAGVGALAGKVMIAFLRVQVLGLPWTRVTQTLFGVQALTSVLVSMAAAGLALIVWSRI
                     RHLHLTRLLSVRA"
     gene            complement(3020..3454)
                     /locus_tag="PKI07_13255"
     CDS             complement(3020..3454)
                     /locus_tag="PKI07_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52048.1"
                     /translation="MEPVPRRVGCGVYFKGQFVARCFRKHDFYKFLEEEGVDWRSKLS
                     KRLLPDDALLVIVRETLFIIEVKYQQVAGSVDEKLQTCDFKRKQYVKLVQGLGLKVEY
                     VYVLGEWFKDPAYKDVLDYINSVNCHYRFGSLPLAWLGLPEP"
     gene            complement(3634..>4486)
                     /locus_tag="PKI07_13260"
     CDS             complement(3634..>4486)
                     /locus_tag="PKI07_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52049.1"
                     /translation="GKTKPSPQSLLPLLHTLDRDRGAAKACGKLPSPRNLSNMKQHEA
                     VIETMARLGGVATLGQLYKEVFKIADCTWQTKTPLATVRRIVQTRKEIYKIKPGLYGL
                     SARRRDIEARGILAETSENKGSAAVADFGHTYYQGLLLLVGKLKGLGCWSPNQDKNKM
                     FLNSNLGALRTYESIPRFSYDGLVNRSATVDVIWFNERHMPNSFFEVEDHSDIQNSLI
                     KFNDLQDFYARMVIVSDATRRREYERKVKYSSFRDIAGRIAFLAYGSLVEQYERAVSL
                     SKMETVL"
BASE COUNT          742 a         1510 c         1329 g          905 t
ORIGIN      
        1 aggacgttcc aaggcgggag ggagaggacg cgaacgttcg gcaaagccgg aacgggcggc
       61 cgggtgttgt cgcgtgccct ggtttttggg cgggatggag ccacggctgg cgggggcatt
      121 ttccctcccg ggtcggggtt gccgaaaggt agctgcttgg gttgtcttca aagctggccg
      181 gtcgccttga gttgatagag gttttcatcc cggtcccctg aactcgaaag gcggctttgg
      241 atttgggaaa accttttctc ccgcagctac cgaaacgcag gcaccgatca cccgaggccc
      301 cgcgccgggt gctgtggcgc ccggagtttt tcttgataac gcgggttcac cgtgcggcgt
      361 tgcgcggcga agcaaagctg tgccgacagc caaagcccgc tccgcaaaac cgcgagccga
      421 tatgaattac cccgggccgt taagaccatg atcgcgctga atggtttccg gcggcgcgtc
      481 tgccggctcc gggcgtttac cctggtggag ttgctgacgg tcatcgccct gatcgcaatg
      541 ctggccgcgc tgctgctgcc ggcgttctcc cgcgccgagg cccggacgcg cgaggccaca
      601 tgcgcgacga acctgcgctc gtgggggcag gcgttttatc tttacgccca cgactgccag
      661 ggatacctgc cccaccccga cgaccagggg cggaataccc cgccgttcac gttcgatccc
      721 cggcacccgg agcacgaatg cggctatgtg gatgttttgc cccccatgat ggggttgcgg
      781 gcctggcgcg atttccccga cggccaaaag ccggccgccg gcgtctggca atgtccggcc
      841 gcccggccgc agcctgattc cgactaccag cgcgccttca agccatcctc ccagggctgg
      901 cacagctacg ccatgaattc ctatctcgcg catgagtttc cgtatgggct gccgttcggc
      961 gcccgcccgc agcccagctt tctgaagctg gagcgttgca cccggcctgc ccggacgctg
     1021 ctcctgtttg aacagacgct ggatccgcgc ccgggctacg ggcaggcggg cggcttggat
     1081 acggcggggt gctacacggc cgaggatccg cgcgcgctgg gcgagcgcca tccgcaccgg
     1141 cgcgccgggc tcgcgggcaa tgtcctttac ctcgacagcc acgttggctg gcgcgaggac
     1201 ctgtggaacc gcgcccgctg acggcgcaac ctggctcacc gctgacggcg tcaacccctg
     1261 ggccagcccg ggtggcgatt acgattccgc gcacccggtg gcgggcgtca agggcggcat
     1321 ccttgacccg gacctccacg accgcttctt ctcctgggat atcaccgcgc tgctcaagga
     1381 ccccgccacg cgggcggcgc tccagaattg cggcgcgctc cttaaaatag acgaaacccc
     1441 tccgccggcc agcggcacgc cccgcgcgcc cttcaccagc gcgtatgacc cgggctaccc
     1501 gtcttcccac tggccaaccc ttcaatgcac gctggccccg gtgctggacg gcgttgcgat
     1561 tgcgggcggc gccgtgtcgt ttgcgatcaa cggcctcatt cccggcaccg ccaacctcgt
     1621 cgagcgttcg cttgatctcg ccgccgccga ttggactccg gtggcctcgt ttgtcgccgc
     1681 gggcgcttcc accaactggt cggaaccctt gttgcccgga gggagccagg ccttctaccg
     1741 cctccgcggc ccggaatgaa cgcccccgga ttcccctccc gctcccggtt gccgcccgtg
     1801 aaccccgctc cgcgcgaagc gctcatcgcc cgccgcgcgc ctggccaccc ggcactgggt
     1861 tttcattggc tcgatggtgg cgctcgattt cgccgccggc cagcttgcga aatccatcct
     1921 gcaggcctcg ggcgtgggcg gcttcatccg cctcgacatg ctcatccccg tcactctctg
     1981 gatgcttacc cggttggtgg tggaccgctt tggcgtcctg gccgcctacc aattcgcctg
     2041 gggaatgctg gccctgttcg cgtttccggg cgcgatactg cccggacccc tcaagcttat
     2101 tcccgccgtc ctccagggcc tgatttgcga cgcgggcttc tcgctgctgc cccgctgccc
     2161 gcgcgcccgg gtcctggtcg ccgccggggt cggcgccctg gccggcaaag ttatgattgc
     2221 cttcctgcgt gtccaggtgc ttggcctgcc ctggactcgg gtcacgcaga cgctgttcgg
     2281 ggttcaagcc ctgacctccg tgctggtgag catggccgca gcggggttgg cgctcatcgt
     2341 atggtctcgc atccgccatt tgcacctcac gcgcctgctg agcgtccggg cctgaccctc
     2401 acccaaccct ttgccggtgg cctccgctgt ccagtgactc ctcatcatca aaacgcccga
     2461 acctgggtat ttcacacttc gcacgaaata tcaacagacc ggcgcgcggg catcttgccc
     2521 gctcagtggg ccgtttcatg aatgcgctca cgcagcggcg gctggcccgt agcgcagatt
     2581 cgtgatctgc tgtatcgcag aattgcactc tgcagagcgc cggacagtcc gaggcccccg
     2641 caaccgtcca gcgcgctgcc gattacagaa tcggcgatgc agcagactgc ggatttgcgc
     2701 tacgactccc ggccagccgc acactggcct tctgccgacc tacccccgca ttccgtcggc
     2761 gcatccgcgc ttcctgccgg tctaccctcg cttcctgccg aggaacgagc gtttcctgcc
     2821 ctcgtacggt gacgttctca gggcataatg tcttctcctc gaggcaagaa ctcatttcct
     2881 cagggcagcc cccgtctttg ccggcagaaa cccgcattcc tggggcaaac ataccgctct
     2941 tgagagccaa aacaggcgat tcccgggccg ataccgcccg tttcttgagg gaacgcccgt
     3001 tttgctcggc cccccagcct tatggctcag gaagaccgag ccacgccaaa ggcagagacc
     3061 cgaaccggta gtgacagttg acgctattga tgtagtccaa gacgtccttg tacgccggat
     3121 ccttgaacca ctccccgagg acatacacgt actccacctt gagtccgagg ccctgaacga
     3181 gcttgacgta ttgcttgcgc ttgaagtcac aggtctggag cttctcatcc accgacccgg
     3241 ccacctgctg gtatttgacc tcgataatga agagcgtctc gcgcacgatg accaacaggg
     3301 catcgtccgg cagaagccgc ttcgacagct tcgagcgcca gtccacgccc tcttcttcca
     3361 ggaacttgta gaagtcgtgc ttccgaaagc accgggctac gaactgcccc ttgaaataga
     3421 cgccgcaccc caccctcctg ggaacgggtt cgatctcata tcccgacgtg gtccgagcaa
     3481 ctatgtccga atctccctgt ttcctgactg caaccccgag ggcgttcgca gtgcgcctct
     3541 tgtagtaaac cagtgtcagg gccggatgag cgattaccac gaacttggca atctctggcc
     3601 gcggagcgcg agccgcgtgg ggacgcgaac ccatcagagc acagtctcca tcttgctgag
     3661 cgagacggcg cgctcgtact gctcgacaag cgagccataa gctagaaacg cgatacgccc
     3721 tgcaatgtcc cgaaacgagc tgtattttac cttgcgctcg tactccctgc gccgcgttgc
     3781 atctgacaca atgaccattc gtgcgtaaaa atcctgcaag tcgttgaact tgatgaggga
     3841 gttctgaatg tctgagtggt cttcgacctc gaaaaaggag ttcggcatgt ggcgctcatt
     3901 gaaccagatg acatcaaccg tcgcgctgcg gttgaccaga ccgtcatacg agaaacgagg
     3961 tatgctctcg taagtgcgca gtgcgccaag gttgctgttg agaaacatct tgttcttgtc
     4021 ctgattgggc gaccagcagc ctaacccctt gagtttgccc acaaggagga gcaagccctg
     4081 ataataggta tggccgaaat ctgccaccgc agcggagcct ttgttctcgc ttgtttccgc
     4141 cagtatccca cgcgcctcga tgtcccgtct cctggcagac aatccgtaga gtccgggctt
     4201 tatcttgtag atttctttgc gcgtttgaac gatgcgccgg acggtcgcca gcggggtctt
     4261 tgtttgccag gtgcaatccg caatcttgaa gacctctttg taaagctggc cgagcgttgc
     4321 aaccccgccc aacctcgcca tggtttctat aaccgcttcg tgctgcttca tgttgctgag
     4381 gttacggggg gagggaagct tgccacaagc cttcgctgcg ccacggtctc tgtcgagtgt
     4441 gtgaagtagc ggcaggagag attgggggga gggtttggtt tttccc
//
LOCUS       DAOPQG010000166         4463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_21193_length_4463_cov_16.569646, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000166 DAOPQG010000000
VERSION     DAOPQG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21193_length_4463_cov_16.569646"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..2444)
                     /gene="uvrB"
                     /locus_tag="PKI07_13265"
     CDS             complement(300..2444)
                     /gene="uvrB"
                     /locus_tag="PKI07_13265"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOC52050.1"
                     /translation="MFELEAPYQPAGDQPQAIAKLTDGLRAGAKHQVLLGVTGSGKTF
                     TLANVIRNVNRPTLVISHNKTLAAQLYAEFKGFFPRNAVEYFVSYFDYYQPEAYIPRT
                     DTFIEKDSSINEEIERLRLSTMSSLFARRDVVVVASVSCIYGIGSKEDYEAMIIPLRT
                     GIDLTRDHFLHRLVDLQYTRNDIEFARGQFRVRGDTVELCPAGREDALRVEFFGDQVE
                     RLTRFEPLTGHRLEELAAVTIYPGKQFVTPAEKLRPAVLAIREELGERIAWFEQHGKL
                     LEAQRIKMRTEYDLEMLEEMGFCSGIENYSRHIAGRPPGSRPCTLFDFFPADYLLVID
                     ESHATVPQIGGMYNGDRSRKTVLVDYGFRLPSALDNRPLNFAEFQALQNQVIYASATP
                     GAQELEWARQSALGGADAGEAPARIPRRARGPAADAVVPGITPPGVVELVVRPTGLVD
                     PKVTLKPLTGQIDDLIHECRRRAEAKERVLVTTLTKRTAEELTDYLREIGIQVQYLHS
                     EIDAIERVEILRALRKGEFDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSETSLI
                     QTAGRAARHLHGEVILYADIQTQSIQRFLAISEYRRQKQLAYNAEHHITPRSVNRAVE
                     ESLSSYQETTRQAQAMLAEAGVDIDLNDTIRELEEEMLAAANNLEFEKAALLRDQVRE
                     LRRALPDAPPDGKPKPGAARPVSYRKRKRKPA"
     gene            2666..>4463
                     /locus_tag="PKI07_13270"
     CDS             2666..>4463
                     /locus_tag="PKI07_13270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOC52051.1"
                     /translation="MNFKNYRKLIALGSLCLTTLAAKAVAPGPYEILPFADGFIQEAY
                     VDLLAGAGIADWTGWSGSSFISPNAYNTHIGTDFSMQTGTPLYAAAAGTVIATYSGYP
                     ANDHSDPYYGNYVKIAVDGLAPNGEAINLIYGHMLSTSVSVGQRVNVGDQVGLSDNTG
                     LSDSEHLHFESDLRSSGAATCPLYWAHFKYPIMFNPDGTHQLGRVVKVTAASTAIRNG
                     RFDSSTQIGTAWQNQLYFCSYPKRGYYHVFIPNNTSYRSGWLRATDVEEVFTGTVIQA
                     LPDSGTYVHLGQLAAKYPIRATASDSAAQIGEIVFGGGRFVADQTSNGYYRIPVPGAS
                     ATWGWVKPTSRMVVYPQLTNPNLNLASLPNNDFPITENFTATGKSMFGRPKFNRSTVK
                     AFSPAAPGGDGKALFMTDKTNYGTGTCESVTVGKPGHRNYYVQCAVYFSYQPSYLGSG
                     QWERYGIYLRDDGFAGLDTTYEGAGNGYALLWDNDDGRLRAGKLVDGVITDFLPTAAY
                     VTGSGWHTLRLEANGTSIKYYLDGNLLINATDSTFHSGQCGIGYSSHTGGSNPAARGA
                     YFDNFIADSLDPALPVITVQPASTTRDPGQTAT"
BASE COUNT          791 a         1456 c         1372 g          844 t
ORIGIN      
        1 tgaaggcagg gtatttccaa aggatctgga ttatggagct ggatgcctga aaaggccaaa
       61 acgaggggcg aggacgggag gataattgct aggttcagta aaaacagcta gttatgggaa
      121 cggggctgac aaaaggcggc ttttcaaggt tgggagggcg ttcggatggc ttttgccatt
      181 aaaaccaagg attggcgcgt tcttttggat tgaattgggg gtgaaacctg aatcgttccg
      241 gctgaaaggc catatcccgg ctttgggaac aacccaaccg gcaaacctgc ggccgggaat
      301 cacgctggtt tgcgtttgcg tttgcggtag cttaccggcc gggccgcgcc tggtttgggc
      361 ttgccatcgg gcggagcgtc cgggagggcc cgcctcaatt cgcgaacctg gtcgcgcagg
      421 agcgccgctt tttcgaactc caggttgttc gccgccgcca gcatctcctc ctccagttcg
      481 cggatggtgt cgttcaggtc tatgtccacg ccggcctcgg ccagcatggc ctgcgcctgc
      541 cgggtggttt cctggtagga cgaaaggctt tcctccacgg cgcggttcac gctgcgcggg
      601 gtgatatggt gttcggcgtt gtaggccagt tgcttctgcc gccggtattc cgagatggcc
      661 aggaaccgct ggatgctttg cgtctggata tcggcataga ggatcacctc gccatgcaaa
      721 tgccgggccg cgcggccggc cgtctggatc aggctcgtct cgctgcggag gaagccctcc
      781 ttgtccgcat ccaggatggc caccagcgag acctccggca gatcaagccc ttcgcgcagc
      841 aggttgatgc ccaccagtac gtcaaactct cccttgcgca aggcacgcag aatctccacc
      901 cgctcgatgg cgtcaatctc gctgtgcaga tactgcacct ggatgccaat ctcgcgcagg
      961 taatcggtca actcctcggc ggtgcgcttg gtgagcgtgg tcaccaatac ccgctctttg
     1021 gcttcggccc gccggcggca ttcgtggatc aggtcgtcaa tctggcccgt cagcggcttg
     1081 agcgtcacct tgggatccac caggccggtc gggcggacga ccagttcgac gacccccggc
     1141 ggggtgattc cggggacgac ggcgtccgcg gccgggccgc gggcgcgccg gggaatccgc
     1201 gccggggctt ccccggcgtc cgccccgccc aacgcgcttt gccgcgccca ctccagttcc
     1261 tgggcgccgg gcgtggcgct ggcgtagatc acctggttct gcagggcctg gaactccgcg
     1321 aagttcagcg gccggttgtc cagcgcgctg ggcagccgga aaccgtaatc caccagcacg
     1381 gtcttgcgtg agcggtcgcc gttatacatc cctccaatct gcggcaccgt ggcgtgcgat
     1441 tcatcaatca ccagcaggta atcggccggg aaaaagtcga acaaggtgca ggggcgcgag
     1501 ccgggcgggc gcccggcgat gtgccgcgaa taattttcaa tgcccgagca gaaccccatc
     1561 tcctcgagca tctccaggtc gtattccgtg cgcatcttga tccgctgcgc ctccagcagc
     1621 ttgccgtgct gctcgaacca cgcgatgcgt tcacccagct cctcccggat ggccagcacc
     1681 gccggccgca gcttctccgc gggcgtcaca aactgctttc ccgggtaaat cgtcaccgcc
     1741 gccagctcct ccagccggtg tcccgtcagc ggctcaaacc gcgtcagccg ctccacctgg
     1801 tccccgaaaa actccacccg cagcgcgtcc tcgcgccccg ccgggcataa ctccaccgtg
     1861 tccccccgca cccggaactg cccgcgcgcg aactcgatat cgttgcgcgt gtattgcagg
     1921 tccaccagcc ggtgcaggaa atggtcgcgg gtcagatcga tccccgtgcg caggggaata
     1981 atcatcgcct cgtaatcctc cttgctgcca atgccgtaga tgcaggacac gctggccacc
     2041 accaccacgt cccgccgggc gaacaggctg ctcatcgtcg agagccgcag ccgctcgatc
     2101 tcttcgttga tgctcgaatc cttctcgata aacgtgtccg tgcgcggaat gtaggcttcg
     2161 ggctggtagt aatcgaagta gctgacgaag tattccaccg cgttgcgcgg gaaaaagccc
     2221 ttgaactcgg cgtaaagctg cgccgccagc gtcttgttat gtgaaatgac cagcgtgggg
     2281 cggttgacgt tgcggatgac gttggccagg gtgaacgtct tgcccgagcc ggtgaccccc
     2341 agcaggacct ggtgcttcgc gcccgcccgc agcccgtccg tcagcttcgc aatcgcttgc
     2401 ggctggtcgc cggcgggctg gtatggcgct tcgagctcga acatccaccg ccaaactacg
     2461 tgcggcgccg ggcggcgtca acgcgccggg gccgcccccg cccgcccgcg cctgccgccg
     2521 cgcgcccgcg gggcgcgggc ggccgccaac cggccgtaaa ttattgttcg cggggacttg
     2581 ttttcccggg caaaccgggt aaaatcatgg gcgtaatgat aaatcatgcc gggttttgcg
     2641 cgtagaggcc tggcgccatg accttatgaa cttcaagaat taccggaaac tgattgccct
     2701 cggctccctt tgtctgacga ccctcgcggc gaaggccgtg gccccggggc cgtatgaaat
     2761 cctgcccttt gcggacggct ttatccagga ggcttatgtg gatctgctgg cgggcgcggg
     2821 catcgccgat tggacggggt ggtccgggtc gagctttatt tcccccaatg cctacaacac
     2881 ccatattggc acggatttct cgatgcagac gggcacgccg ctttacgccg ccgccgccgg
     2941 cacggtgatc gccacctact cgggctatcc ggccaacgac cacagcgacc cgtactatgg
     3001 caactacgtc aaaatagccg tggacggcct cgcgcccaat ggcgaggcca tcaacctgat
     3061 ctatggccac atgctttcga cctcggtcag cgttgggcag cgggtcaatg tgggcgacca
     3121 ggtcgggctt tccgacaata ccggcttgtc ggactcggag catctgcatt ttgagagcga
     3181 cctgcgcagc tcgggcgcgg cgacctgccc gctttactgg gcgcacttta aatacccgat
     3241 tatgttcaat ccggacggca cgcaccaact ggggcgggtg gtgaaggtga ccgccgccag
     3301 cacggcgatt cgcaacgggc ggttcgacag cagcacgcaa atcggcaccg cctggcagaa
     3361 ccagctttat ttctgcagct atcccaagcg cggttattac cacgtcttca tcccgaacaa
     3421 tacttcgtat cgctccggct ggctgcgggc gaccgatgtc gaggaggtgt tcacgggcac
     3481 ggtcattcag gcgctgcccg acagtggaac ctacgtccac ctgggacagt tggcggcgaa
     3541 atacccgatc cgggcgacgg ccagcgacag cgcggcgcag atcggggaga ttgtattcgg
     3601 cggcggccgg tttgtggccg accagacttc caacggctac taccgcatcc cggtgccggg
     3661 cgcttcggcc acgtggggct gggtcaagcc cacctcgcgc atggtggtgt atccgcagct
     3721 caccaacccc aatctcaacc ttgccagcct tcccaataac gactttccca tcaccgaaaa
     3781 ctttaccgcc accggcaagt ccatgttcgg ccggcccaag ttcaaccgtt ccacggtcaa
     3841 ggcgttcagc cccgcggccc cgggcggaga tgggaaggcg ctgttcatga ccgataagac
     3901 caattatggc accggcacgt gcgaatcggt gaccgtgggc aaaccgggcc accgcaatta
     3961 ttatgtgcag tgcgccgtct atttcagcta ccagccgtcc tatctcggca gcggccagtg
     4021 ggaacgctat ggcatttacc tgcgcgatga tggcttcgcc gggctggata ccacctacga
     4081 gggcgcgggc aatggctacg cgctgctttg ggacaacgac gacggccgcc tgcgggccgg
     4141 caaactggtg gatggcgtca ttaccgattt ccttcccacc gcagcctacg tgaccgggag
     4201 cggctggcat acgctgcgcc tcgaagccaa cggcacgagc atcaagtatt acttggatgg
     4261 gaatctgctg atcaacgcca ccgactcgac gttccactcc gggcaatgcg gcatcggcta
     4321 cagcagccat accggcggca gcaatcccgc cgcccgcggc gcttacttcg ataactttat
     4381 tgccgactct ctcgacccgg cgctccccgt catcacggtg cagccggcca gcacgacccg
     4441 ggatccgggg cagacggcca cgt
//
LOCUS       DAOPQG010000167         4438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_21352_length_4438_cov_17.342003, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000167 DAOPQG010000000
VERSION     DAOPQG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21352_length_4438_cov_17.342003"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKI07_13275"
     CDS             complement(<1..331)
                     /locus_tag="PKI07_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52052.1"
                     /translation="MKMLTSPRTGSLGAETYYQSPFGVCARRRTVPRDRPSNRKAAAR
                     SYFGISSREWGLKLSEAQRERWNAAALHVPSRPWMGQYSHLSGQQFCVQINSTLRGLG
                     LAPVEEPP"
     gene            932..2464
                     /locus_tag="PKI07_13280"
     CDS             932..2464
                     /locus_tag="PKI07_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC52053.1"
                     /translation="MKRNVIIPPGRGFCLGLALLAGLVSPGAGAAPAPPARPDILFIM
                     PDQMRGDCLSLLGHPAVRTPHLDKLAAEGALFRRAYSTCPSCIPARAALLTGLFPATR
                     GVVGYAARPISYPTLPQRLAAAGYQTVLVGRYMHQKPLEESYGYQVEIRGSVHVDDDA
                     YDRFLKQAAPATGGIRNLLTRQGLSYNGWQAAPWPLADDLHPTAWTVRQARKILEELS
                     AERPVFLTASFYAPHPPLFPPRRYFDAYRRQKLPAPARGDWVDWPALSPAGDKPGHRV
                     LLEGEALRAAEAGYFGLIQHLDDQIAPLMAAFKKRSRKARRPWVIMFTSDHGEMLGDH
                     GFYRKCEPYEGSANIPFLVAAAPELGFKRGLRSELPVCLEDVMPTLLALAGASCPQPM
                     DGVSLAPVLRGETQRLRECLHFEHAPCYSPLQAFQALTDGQFKYIWRPLDGSEQLFDL
                     ARDPREEHDLARGPSRRALLGQWRARMVRQLAGRPEGFSDGTHLIPGRPYPALQAKLK
                     EL"
     gene            2461..>4438
                     /locus_tag="PKI07_13285"
     CDS             2461..>4438
                     /locus_tag="PKI07_13285"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOC52054.1"
                     /translation="MMHRCSWLTLTVLLTLAVSAPAGRAAGFHAQGLRCEYRANPLGI
                     DTPQPRLSWRLEASERGQRQTAYRVLAATRPKLLAEGRADLWDSGKVASSESVHVVYG
                     GRPLRAGQRVYWTVRAWDRDGRASRYSETAWWEMGLLAPADWQADWITRRRPAPLAES
                     QLFADDPAPLLRKEFVVEKKIRRARIYVSGLGYYELRLNGRRVGDQVLDPGWTTYSQR
                     VLYSTYDVTACLRRGRNAWGVMLGNGWFNPLPLRLWGHVNPRENLTIGEPRVIAQLRI
                     ELADGTTQTLGTDVSWKTGPGPIRRNSVYLGEVYDARREQPGWDQPGFDDSNWEPAQT
                     ARGELGPLQAQDAPPIRVTRVLQPVKLTEPKPGVHIFDFGQNFAGWARLRVQGPAGTR
                     VKLRYGELLYPDGTLNGMTAVCGQIKGGGRDYRYDGQGAPKTAWQMDDYILKGQGREI
                     YTPRFTFHGFRYVEVTGYPGQPTLASLDGLRLNADVAPVGSFACSNEQFNRIQEMVLW
                     TELSNLFSVQSDCPHREKFGYGGDIVASGEMALFNFDMGRFYAKAVRDLADAVRPNGG
                     FTETAPFVGISDEGLGERSGPVGWGTAHPFLLWGLYQHYGDRRLLEENYEAARRWLGL
                     LQSQARDGILDNGISDHESLVPKPRALTGTGFYYL"
BASE COUNT          695 a         1379 c         1569 g          795 t
ORIGIN      
        1 cgggcggttc ctccaccggc gccagaccca gcccccgcag ggtgctgttg atctgaacgc
       61 agaactgctg cccacttaag tgcgaatact gccccatcca gggccggctg ggaacgtgca
      121 gcgccgcggc gttccagcgt tcgcgctgcg cttcgctgag tttgaggccc cactcccgcg
      181 aggagatccc gaaatacgac cgtgcggccg ctttgcggtt agaaggccgg tcccggggaa
      241 cggtgcgccg ccgggcgcac acgccgaagg gggattggta gtaggtttcc gcgcccagac
      301 tgcccgtgcg cggagatgtg agcattttca tagttaatta cctttctgtt atcgccccgg
      361 agagcgactc ggcttcctgc cactccttgg actgaaagtc ccagccacca ccactccgag
      421 ggttttgggg aaggctggcg ggcgggaggt cttcgcgcaa gggcaatttt tggggggtgg
      481 tggtggcagg ccggtgcgga gcgccgcgtg agggcacgcg gcctacaggg ggcgggatga
      541 tacgaggggg cctgtgggtt gtcgggagtg cggtgttgcg agagggcatg aggtttgccg
      601 cggcgcgctt ccatttcctg caggccgcgt gcccatacgc ggcgctcggg ggtctttcgt
      661 ccggcggctg agaaaaacac ctgcgcgtcc acccggattg cctgcgggaa agcctgctcg
      721 ccttggaggg cgatctcacc tccgctgttc ccaatggccc aagccttggg cggcccgacc
      781 aggcacccgc tccgcagggc ttaaccgccg cccaacccgc agcctcaacc actccggctc
      841 atgatctaca aagccaaagg ctctggcctt gctcccgggt tggcgaattt tggcctgacg
      901 cagccgaacc gattcgctac gctgcgcgct tgtgaaacga aatgtaatca tcccgccggg
      961 gcgtggattc tgccttgggc tggcgctgct ggcggggttg gttagccccg gcgccggggc
     1021 tgcgccggca ccgccggcca ggccggacat tcttttcatc atgcccgacc agatgcgcgg
     1081 agattgcctg tcgcttttgg ggcacccggc ggtccgcacg ccgcacctgg acaagctggc
     1141 ggcggaaggg gcgttgtttc gtcgcgccta ctcgacctgc ccgtcctgca tccccgcgcg
     1201 cgccgcattg cttacgggtc tcttcccggc cacccgtggg gtggtgggct acgcggcccg
     1261 gcccatttcc tatcccaccc tgccgcaacg gcttgcggcc gccggttacc agaccgttct
     1321 ggtggggcgt tacatgcacc agaagccgct tgaggaatcc tacggctacc aggtggaaat
     1381 ccgcggctcg gttcatgtgg acgacgatgc ctacgaccgg tttctaaagc aggcggcgcc
     1441 cgccaccggc gggattcgca acctgctgac caggcagggc ttatcctaca atggctggca
     1501 ggccgcgccg tggccgctgg cggatgattt gcatccgacg gcatggacgg ttcgccaggc
     1561 ccgcaaaata ctggaggagc tgtccgccga acggccggtc tttctaacgg cgtcatttta
     1621 tgcgccccac ccgccgcttt ttccccccag gcggtatttt gatgcctatc ggaggcaaaa
     1681 gctgcccgcc ccggctcgcg gcgattgggt ggattggccg gccctgtcgc cggcgggcga
     1741 caagcccggg catcgcgtgt tgctggaggg cgaggctttg cgggcggcgg aggcgggcta
     1801 cttcggcctg atccaacacc tggacgacca gattgcgccg ctgatggcgg catttaagaa
     1861 acggagccga aaggcccggc ggccctgggt gatcatgttc acgtccgacc acggcgagat
     1921 gctcggagac cacggcttct accggaaatg cgagccgtat gaagggtcgg ccaacatccc
     1981 attcctcgtg gccgcggcgc cggaactggg ttttaagcgg ggcctgcgct ccgaactgcc
     2041 ggtctgcctg gaggatgtga tgccgacgtt gctggccctg gccggcgcga gctgcccgca
     2101 gccgatggat ggagtgagcc tggctcccgt cctgcgcgga gagacgcaaa ggcttcgcga
     2161 gtgcctgcat ttcgagcatg cgccctgcta cagtccgctc caggctttcc aggccctgac
     2221 ggatgggcag ttcaaataca tctggcggcc gctggacggc tccgagcagt tatttgacct
     2281 ggcccgggac ccgcgggagg agcatgacct ggcccggggg ccctcccggc gcgcgttgct
     2341 cgggcaatgg cgcgcgcgga tggtccggca gcttgccggc cgtccggaag gtttctcaga
     2401 tggaacccac ctgattcctg gccgcccgta cccggcgctc caggcgaagt taaaagaatt
     2461 atgatgcacc gctgttcctg gctcacgctc acggttttac tcacccttgc cgtctcggct
     2521 ccggccgggc gggcggctgg atttcatgcg caaggcttgc ggtgcgagta ccgcgccaat
     2581 ccgctgggga ttgatactcc tcagccgcgg ttgagctggc ggttggaggc gtcggaacgc
     2641 gggcagcggc aaacggctta tcgcgtgctg gccgccaccc gcccgaagct gctggcggag
     2701 ggcagggcgg atttgtggga cagcggcaag gtggcctcga gtgagtcggt gcatgtcgtc
     2761 tatggcgggc gcccgttgcg cgcgggccag cgggtctatt ggacagtgcg cgcctgggat
     2821 cgcgacgggc gggcctcgag gtacagtgag acggcgtggt gggagatggg cttgctggcg
     2881 ccggcggatt ggcaggcgga ctggattacg cgccggaggc ccgcgccgct tgcggagtcg
     2941 cagttgttcg cggacgatcc ggcgccgctg ctgcgcaagg agtttgtggt cgaaaagaag
     3001 attcgccgcg cccggattta tgtgagcggc ctgggctact acgagttgcg cctcaacggg
     3061 cgccgggtgg gcgaccaggt gctcgacccc ggctggacga cctactcgca gcgcgtgctc
     3121 tattcgactt acgacgtgac ggcttgcctg cggcgggggc gcaacgcctg gggagtgatg
     3181 ctgggcaacg ggtggtttaa cccgctgccg ctgcggttgt gggggcatgt caacccccgc
     3241 gagaacctga ccatcggcga gccgcgcgtc atcgcgcagt taaggatcga gctggcggac
     3301 ggaaccacgc aaacgctggg cacggatgtc agttggaaga ccggccccgg ccccatccgg
     3361 cgcaatagcg tgtacttggg cgaggtgtat gacgcgcggc gggagcagcc cggctgggat
     3421 caacccggct ttgatgattc gaactgggaa ccggcgcaaa ccgcccgcgg cgaactgggc
     3481 cccttgcagg cgcaggatgc gccgccgatt cgcgtgacgc gcgtgctcca gcccgtcaaa
     3541 ctcaccgagc cgaagccggg cgtccatatc tttgatttcg ggcaaaactt tgcgggctgg
     3601 gcgcggctgc gcgttcaggg gccggcgggc acgcgcgtga aactgcgcta cggagagctg
     3661 ctttacccgg acggcaccct caacgggatg accgccgtct gcggccagat taaaggcggc
     3721 ggccgcgact accgttacga cgggcagggc gcgcccaaaa cggcctggca aatggatgac
     3781 tacatcctca aggggcaggg gagggaaatc tacacgccgc ggtttacgtt ccatgggttc
     3841 cgctatgtcg aggtgacggg ctacccgggg cagccgacgc tggcatcgct ggacgggttg
     3901 cggcttaacg cggatgtggc gccggtgggt tcgttcgcct gctccaacga gcagttcaac
     3961 cggatccagg agatggtgct ctggacggag ctgagcaacc tgttcagcgt gcaatccgat
     4021 tgcccgcacc gcgagaagtt cggctacggg ggcgacatcg tagcgtccgg cgagatggcc
     4081 ctgttcaact ttgatatggg gcgtttttat gccaaggcgg tgcgggatct ggccgacgcg
     4141 gtgcgcccga atggcgggtt cacggagacc gcgcccttcg tggggatcag cgacgagggg
     4201 ctgggggaac gctcggggcc ggtgggctgg ggcaccgcgc acccgtttct cctgtggggg
     4261 ctttatcagc attatggcga ccggcggttg ctggaggaga attatgaggc ggcgcggcgg
     4321 tggctggggc tgctgcaatc gcaggcgcgg gacggcatcc tggacaatgg catcagcgac
     4381 catgaaagcc tcgtgcccaa gccgcgcgcc ctgacgggca ccggcttcta ctacctca
//
LOCUS       DAOPQG010000168         4422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_21458_length_4422_cov_14.251431, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000168 DAOPQG010000000
VERSION     DAOPQG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21458_length_4422_cov_14.251431"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(643..1029)
                     /locus_tag="PKI07_13290"
     CDS             complement(643..1029)
                     /locus_tag="PKI07_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOC52055.1"
                     /translation="MSEAQLSQYLPVLMLGMLAVVFSFGMLGMSVVLGKRGKRPAVKD
                     TPYECGMVPMGEGTPRFSIKFYLVAMLFILFDIEVIFLYPWAVIYRDMLKEGGGAIFG
                     AMAVFLAVLLVGYLYALKKQAFAWKN"
     gene            1123..1965
                     /locus_tag="PKI07_13295"
     CDS             1123..1965
                     /locus_tag="PKI07_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOC52056.1"
                     /translation="MYPTVVTRNPTAVQAEVETAYLVIFPHADLAFVGRFFDWAIDCF
                     SGKHRDYQAVDARYHDFEHTLQGVLCLTRLLRGRHLANAQPRLNERMFQLALAAILFH
                     DTGYLKKRDDIEGTGAKYTLTHVQRSAEFTAQFLQEKGFGATDIQAVQNMISCTGVNA
                     TLEAITFQSELERIAGSCLATADLLGQMAAEDYVDKLPVLFSEFAEAALYTKDQTSFV
                     ASFSSAEDLIRKTPDFWRKIVRVKLDRDFSGVHIFLNDPYPAGPNPYFERIEANLARL
                     RERL"
     gene            complement(2065..4302)
                     /locus_tag="PKI07_13300"
     CDS             complement(2065..4302)
                     /locus_tag="PKI07_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOC52057.1"
                     /translation="MHWKPYILKGVVLGGAMVFVSSLLQAQTNQPLVLADVNGFNDTE
                     LYPPGNLQLVTDGGARWEPAPGTPAQIVPLVGDSHGQVLRRVQTGTDNMDFLHFPPVS
                     DGTLTVSLEARASTAATRTLDVFLLPDNGGETCLLGWGTVSNQLAYYDGSQWRAVRHL
                     DTNWHHVEMISYLSGPRFGTWDLKVDGSLLATNLPWRNHHPPNKAYNRLRLGGIRGVT
                     GSYADVDNLVITAEGATGAPEVFYLTNISVLTNEFRLSFETQTNAEYCIEYTRALTNE
                     VWSPLAIVPGSGLVAWFTNETATNAARFYRVKKLPPPGYADGYRGIWFTLGQFSSYGD
                     KYSGGLGTYTANHIPLAIYAPQVNKTFFTYGGTLKDKRHLLIMVSEYDHATGQVPRPT
                     LVLDKHGVNDPHDNASLALDDQGFLWVFVSGRAAARPGWIFRSRTPYSSASFDFIRQD
                     EMTYPQPRHIPGFGFFTLFTKYTAGRELYWMTSSNGVTWSPHRKLAGIGGHYQVSNVR
                     ADGLVATFFNRHPGGNVDKRTDIYYAQTADYGATWTTAAGVPLVLPLTTTNNPARIFD
                     LASEGKLMYTCDLNFDTNGHPVLLYIVSRHYQPGPGGDPREWTVARWNGTQWLTSVVC
                     TSDHNYDMGSLYILPDKWLIIGPTQVGPQPWQTGGEMALWTSVDQGQTWTMTRQLTAN
                     SLYNHTYARRPLNAQDPFFAFWADGQPTQFSPSRLYFGDSSGSNVWRLPYEMTAPLAT
                     PTPLN"
BASE COUNT          996 a         1201 c         1221 g         1004 t
ORIGIN      
        1 cggcggagag cacggcgacg gtggtggcga gcctgctgga tgaatttggg cgaatcccca
       61 gccgcttctt ccgcatcatg attgtcaatt agcgcggcgc caccaaaacg aacaaagtct
      121 tgccaagcct ggcatttggt ttatggttaa ccataccggg ctatgttaga gtgtgttaga
      181 ccgcttttgg cggatgctgt tctctcaggt ttggcggtat tggcggctgg acttgtgggt
      241 tacttggggt ggctgtgctt tcgcgaaaag cgccagcgac tgagattgcg tcgcgaacag
      301 gagcgttgcc gccgggacca ttggggatac atttaacact ggcaaggcgc gtttgatttg
      361 cggttgcaaa aaaagattat tcgtgtattt ttaggctcga cgaaatggtt gctatgggtt
      421 agtgttgggt aatcgttctt ttgtccgggt ggctgacgac ctttgcctcc ccaggggttc
      481 cccaccccaa aatacctgcc aaggtccagg ccatccggac tattttttta cgagaacgaa
      541 ccagatggtt atccagcaag aggcaggccc aactccaatg ggtcatcgag agttagcact
      601 gcggttaaac gtttcccccg gacagggtag cgcaatccct cctcaattct tccaagcgaa
      661 ggcctgtttc ttcaaggcgt agagatagcc gacgagcaga acagccagga acaccgccat
      721 tgccccgaaa atcgctccac cgccttcctt gagcatatcc cgatagataa ctgcccaagg
      781 ataaagaaaa atgacttcga tatcgaacag gatgaacaac atcgccacga ggtaaaactt
      841 gatggaaaac cttggcgtac cctcgcccat cggcaccatg ccacattcat aaggggtgtc
      901 tttgacggcg ggccgcttgc ctcgcttgcc aaggacgacg gacatgccca acatgccaaa
      961 ggaaaatacc accgccagca tcccgagcat caacaccggg aggtattgcg acagttgtgc
     1021 ttctgacatg ccccgaatct aaaacctgtt caccttttgg caagctgaat ccggcgtaat
     1081 ttgggcgtgc atttttcgtt ctgtatgcta gcctgaaatt aaatgtatcc tacggttgtc
     1141 acccgcaatc caaccgccgt acaggctgag gtcgaaactg cctatctcgt aatctttccc
     1201 catgctgact tggcgtttgt gggacgcttt tttgattggg ctattgattg ttttagcggc
     1261 aaacaccggg attatcaggc ggtagatgcc cggtaccatg attttgagca taccctccaa
     1321 ggggttcttt gtctgacccg cctgttgcgg gggcgccatc tggctaacgc gcagccccgg
     1381 cttaatgagc gcatgttcca actcgccctg gccgccatcc tttttcacga caccggctat
     1441 ctcaagaaac gcgatgatat agaaggcacg ggagccaagt acacgctcac ccatgtccag
     1501 cgcagcgctg aatttaccgc tcagtttctt caggagaagg ggtttggcgc tactgacatc
     1561 caagccgtgc agaacatgat ttcatgcacc ggcgtgaatg cgaccctgga agccataact
     1621 ttccagagcg aattggaaag aatcgcaggc tcctgcttgg cgactgccga cttgctgggg
     1681 cagatggcag cggaggatta tgtggataag ctcccggttc tcttttccga gtttgctgaa
     1741 gccgccctct acacaaaaga tcagaccagt tttgttgcca gcttttccag tgccgaagac
     1801 ctaatccgca agaccccgga tttttggcgg aagattgtgc gggtgaaatt ggaccgcgat
     1861 ttttcgggag tgcatatctt tctcaacgac ccctatcctg ccggccccaa tccttacttt
     1921 gagcggattg aggctaacct cgcccgcttg cgcgagcggc tttgagagga atacctgcgc
     1981 gtccaagcgg gcgacagaac caccgggaag ccaaggctgg tctgagccgc caaacgcagg
     2041 acacacactt ggctggcaac cagatcagtt taatggtgtt ggggtggcta gcggagcagt
     2101 catctcatac ggcaaccgcc aaacatttga gccggaggaa tccccaaagt acaaccgcga
     2161 aggcgagaat tgggttggtt gtccgtcggc ccaaaacgcg aagaagggat cttgagcatt
     2221 caagggacga cgcgcgtacg tgtgattgta aaggctgttg gcagtaagct ggcgagtcat
     2281 cgtccaggtt tgcccctggt ctacgctggt ccaaagcgcc atctcgcccc cggtctgcca
     2341 gggttgtggt ccaacttggg ttggcccaat gataagccat ttgtccggca gaatgtagag
     2401 gctgcccata tcgtaattgt gatcggatgt gcaaaccacc gaggtcagcc attgcgtgcc
     2461 gttccagcgt gccactgtcc attcgcgcgg atcgccgcct ggcccgggtt ggtaatggcg
     2521 gctgacaata tagagcaaca ccgggtggcc gttggtgtcg aaatttagat cgcaagtgta
     2581 catcaacttg ccttcggacg ccagatcgaa gatgcgggcg ggattattgg tcgtggtgag
     2641 cggcagcact agcggcacac cggccgccgt ggtccaagtg gcaccgtaat ccgccgtttg
     2701 ggcgtaatat atgtccgttc gtttatcaac atttcccccg ggatgacggt taaaaaaggt
     2761 agccaccaac ccatccgcac gcacgttgct gacctgataa tgccccccta tgccggccag
     2821 tttccgatgc gggctccagg tcacgccatt actgctcgtc atccaataga gttcgcgtcc
     2881 ggcggtatat ttcgtgaaca gggtaaagaa cccgaagccg gggatgtgcc gcggctgggg
     2941 ataggtcatt tcatcctgcc tgatgaaatc aaaactggcg ctgctgtaag gcgtgcgact
     3001 gcgaaaaatc cagcctggcc gtgcggcggc acggccgctg acgaacaccc acaaaaagcc
     3061 ttgatcatcc agcgccaggg aagcgttgtc gtgcggatcg ttcaccccgt gtttatcgag
     3121 cacgagcgtt gggcgaggca cctgcccagt ggcgtggtcg tattctgaaa ccatgatcag
     3181 caggtggcgc ttatctttga gcgtgccgcc gtaggtgaag aatgttttgt taacctgagg
     3241 cgcatagatg gccaaaggaa tgtgattggc cgtataggtg cccagaccgc ccgagtattt
     3301 gtcgccgtag ctggagaact gcccgagagt gaaccagata ccgcgatagc cgtccgcgta
     3361 gccgggcggc ggcaacttct tcacccggta aaaacgagcc gcgttagtgg ctgtttcatt
     3421 ggtaaaccaa gctacgagtc cactgcccgg gacaatggct aaaggcgacc aaacctcatt
     3481 ggtcaatgcg cgcgtgtatt cgatacagta ttcagcattg gtttgagtct caaacgataa
     3541 ccgaaactcg ttcgtcaaaa cggatatgtt tgtcaggtaa aaaacctcgg gtgcgccggt
     3601 cgcgccctcg gcggtgatga ccaggttatc cacatcagca taggaacctg tcacgccacg
     3661 gattcctccc aaacgcaacc gattataggc tttgttcggc gggtgatgat tccgccaggg
     3721 cagatttgtg gcaagcaatg agccatctac cttcaaatcc caagtgccaa acctgggacc
     3781 gctcagatag ctgatcattt ccacatgatg ccagttggta tcgagatgac gtacagcccg
     3841 ccattgtgag ccgtcgtagt aggccagttg gttgctcacc gttccccagc ccaacagaca
     3901 agtctcgcca ccgttatccg gcagcagaaa tacatccaag gtgcgagtgg cagccgtcga
     3961 agcccgtgct tccaggctga cggtgagggt tccgtcagag accggcggga aatgcaagaa
     4021 atccatgttg tccgtcccgg tttgtactcg tcgcaatacc tgtccatgac tatcccccac
     4081 cagcgggacg atttgcgcag gggtgcccgg tgccggctcc cacctggccc ctccgtccgt
     4141 cactaattgc agattgcccg gcggatacag ttccgtgtcg ttaaaaccgt tcacatcggc
     4201 caacacgagc ggctgattcg tctgcgcttg caggagtgag gaaacaaaca ccatggcccc
     4261 ccccaggacc acccctttga ggatataagg cttccaatgc attggagaag tctaagattg
     4321 atgcctgtta aggacaagtt tcttttagcc gaaatgattc agattttaag ttgtgtccgt
     4381 caagttcagc aaattggtcg tcatggtttc cactcaaggt gt
//
LOCUS       DAOPQG010000169         4354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_21918_length_4354_cov_14.198418, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000169 DAOPQG010000000
VERSION     DAOPQG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21918_length_4354_cov_14.198418"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..3339
                     /locus_tag="PKI07_13305"
     CDS             244..3339
                     /locus_tag="PKI07_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOC52058.1"
                     /translation="MPHSTVTSIAQTPDGYLWVGTQLGGLARFDGVRFVTFHPGNTAA
                     LQSLEVRQLLVDPGGTLWVGMVDGHLLSRRQGSFHLESQQLPTPLSWIHSLIFSRSNE
                     VMISSFGGWLFHGRSQSGTNRWETFIAPDGDSLSAFCEDPEGRIWYRTRDAWLGVFAN
                     QQFSRMPTNCGLAGLVINALTRDAEGRIWVGTDREIAVWDGRQFINMTPTNGEPEIVV
                     RQLVFTDDRGLWVRDETRLRKCLGRAWVAAAAPWDGHFQPAVKPLEMYGGAQGGIWVV
                     HYSDGIWHVDRSGRVARISHKEGLPANHVESWFQDREGNIWAGLSGGGLVRLRRRTFQ
                     QVWPGESLPERAARSVCEDAAGAMWFGTSGDVFLRWQEGQFSTLVPPVETTSGRDGTV
                     CLDAAGRVWLGTVLNGAWVLETNGLRRPFPASAIGTVVRALFADRAGRVWIGNEFGLY
                     CWENGALKTFSAQEGFGPGLVYAITEGKDGTLWFGTAEGELRQFKGGRFVTYRPQDRF
                     GATRFWALLADHDGVVWIGTLGGGLLRFEEGQFVRYTMRDGLPNEHVSQILEAQRGDL
                     WLGTRAGIARVRKIDLDQFARGETKSVPFVTYGKFDGLPSVECSGGYQPGCWRSRNGS
                     LWFTTAKGVVSVKPKDIPFNPWPPPVVIEEVLVEGKVHSPAVESRRPEAANGAKFAPH
                     RPCLPRVEMGPGRHYLEIHFTGLSFIAPDKVQFKYRMEGVDPGWVEAGNQRLAIYNFI
                     PPGEYRFRVLACNNDGVWNEEGATLALEVRPYFWQTLWFKILGGILLVGGSGGAIGFY
                     ERRKARRKLERVQQQRALERERARIAKDIHDDLGANLTRITLLSDSVRSDLGDTAQAE
                     VHLDRISATARDLTRAMDEIVWAVNPKHDRLDSLVNYLGKFAQDFLSSAGIAYRSEMP
                     IQLPHWPLDSQVRHNLFLAFKEALHNAVKHAAAKEVHVSLTLRKEAFVLTVRDNGCGF
                     RRDDLASSADPALSRLSTGNGLVNMKNRMEGIGGHCELQSKPGAGTVVRFVVRLRHPE
                     K"
     gene            3499..4143
                     /locus_tag="PKI07_13310"
     CDS             3499..4143
                     /locus_tag="PKI07_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC52059.1"
                     /translation="MNTLIKVSIVEDDHAVREIFADWVGHADGFRCVSTYANGERAVA
                     DLPRVRSNIVLMDINLPQMNGVECVRRLKPLLPATQFVMVTVYEDADHIFDALAAGAV
                     GYLLKRTPRKQLLAALREVNAGGSPMSTNIARMVVESFGQPRQIVSPEMQGLSPREQE
                     VLELLARGYLYKEIAEALKISPLTVPSFVRRIYDKLHVHSRAQAVAKVAHWTTR"
BASE COUNT          816 a         1185 c         1430 g          923 t
ORIGIN      
        1 caatcctgcc cgccgcccgg cttgcttccc gaataaatgg cctggcggtt aagctgcgct
       61 gcaggtttgc ttgccagcat cgcgttctga tggtattggc gatctagtca tgcttagccg
      121 gggccaactt ttgtctgccg tattcagtgc cctgatgtgt tgtggccacg ggcgttgagc
      181 gttgccggcc agttgccggg ctcaccggag tatctgattg atgtttggga tactgacagt
      241 ggcttgccgc acagcactgt cacgagcatc gcgcaaaccc cggatggcta cttgtgggtt
      301 ggcacacagc tgggaggact ggctcgcttt gacggggttc gcttcgtcac ctttcacccc
      361 ggaaacacag cagccctgca atcgctcgaa gttcggcagt tgttagtgga cccgggggga
      421 accctgtggg tcggcatggt ggacgggcat ttgctgtccc gccggcaggg gagctttcac
      481 ctggaaagcc aacaactgcc tacgccgttg tcctggatcc actcgcttat cttcagccga
      541 agcaacgaag tgatgatttc cagcttcggc ggctggcttt ttcatggccg ttcccaatcc
      601 ggcacgaatc gctgggagac gttcatagcg ccggatggcg acagcctctc cgcgttttgc
      661 gaagaccccg aaggccgcat ctggtatcgc acgcgagatg cgtggctggg ggtgttcgcg
      721 aaccagcaat ttagccggat gccaaccaac tgcggcctgg ccggcctggt catcaatgcg
      781 ctgacgcgtg atgcggaggg gcgaatttgg gtgggcaccg accgggaaat tgccgtttgg
      841 gatgggcggc agtttatcaa catgaccccc accaatggcg agccggaaat agttgtgcgt
      901 caactggtgt tcacggatga ccgcgggttg tgggtgcgcg atgagactcg tctgcgcaag
      961 tgtttaggcc gggcctgggt ggccgccgcg gcaccgtggg atgggcattt tcagccggcg
     1021 gtcaagccgt tggaaatgta tgggggcgcg caaggcggca tctgggtggt gcattatagc
     1081 gacggcatct ggcacgtgga tcggagcggc cgggtggcgc gcatttccca caaggagggg
     1141 ctgccggcca accatgtcga gtcctggttc caggatcgcg aaggcaacat ttgggccggg
     1201 ttgtccggcg gcggactggt gcgcttgcgg cggcgcacct tccaacaggt ctggcctggc
     1261 gagagcctgc ctgagcgggc ggcgcggtcg gtttgcgagg atgcggcagg cgccatgtgg
     1321 tttggcactt ccggtgatgt ttttcttcgc tggcaggagg gtcagttttc gaccctggtc
     1381 ccgccggtgg aaacaacgtc ggggcgcgac ggaacggttt gcctggatgc cgccggccgc
     1441 gtctggctgg gcacggtgtt aaatggcgcc tgggtgttgg aaaccaatgg cctgcgccgg
     1501 ccgtttccag ccagcgccat tggaacggtg gttcgcgcgt tgtttgcgga tcgcgccggc
     1561 cgcgtctgga ttgggaatga gtttgggctt tattgctggg agaacggcgc gttgaagacc
     1621 ttttccgctc aggaggggtt cgggcccggc ctggtgtatg ccatcaccga aggcaaggat
     1681 ggcaccttgt ggttcggtac cgccgaggga gaattgcggc aattcaaggg cgggcgtttc
     1741 gtcacttacc ggccgcaaga ccggtttggc gccacccgtt tctgggcgct cctggccgac
     1801 cacgatggcg tggtctggat aggcacgctc ggcggcggat tgctgcggtt tgaagaaggc
     1861 cagtttgtgc gctacacgat gcgcgacggc ttgcccaatg agcatgtgag ccagatcctg
     1921 gaagcccagc gcggagattt gtggttgggg acacgagccg gcatcgcccg cgtccgcaag
     1981 atagatctcg atcagtttgc ccgcggcgaa acgaaatctg tgccgttcgt gacctatggc
     2041 aagttcgacg ggttgcccag cgtggaatgt tccggcggct accagccggg ttgctggcgc
     2101 agtcggaatg ggagcctgtg gtttaccacg gccaaaggcg tcgtttctgt gaaaccaaaa
     2161 gatattcctt ttaatccctg gcctcctccg gtggtcatcg aggaagtgct cgtggagggc
     2221 aaggttcaca gccctgcggt tgaatcccgg cggccggagg cggcgaacgg cgcgaagttc
     2281 gctccgcacc gcccgtgcct tccgcgggta gagatggggc ccgggcgcca ctaccttgaa
     2341 atccatttca ccggactgag tttcatcgcg ccggataagg tccaattcaa ataccgcatg
     2401 gagggggttg atcccgggtg ggttgaagcc ggcaaccagc gcctggcgat ttacaatttc
     2461 attccgccag gggagtatcg ctttcgcgtg ctggcctgca ataacgatgg tgtgtggaac
     2521 gaggagggcg ccaccctggc gctggaggtg cggccctatt tttggcaaac gttgtggttc
     2581 aaaatcctgg gggggattct gctggtgggc ggttcaggcg gggcgatagg cttctacgag
     2641 cggcgcaagg cccggcgcaa actcgagcgc gtgcaacagc agcgcgcgct ggaacgcgag
     2701 cgcgcccgca ttgccaagga tatccatgat gatctcggcg ccaacctcac gcgcatcacc
     2761 ctgttgagcg attcggttcg gagcgacttg ggcgacacgg cccaggccga ggtccacctg
     2821 gaccggattt ccgccacggc gcgcgatctc acgcgcgcga tggacgaaat cgtctgggcg
     2881 gtaaacccga agcatgaccg gctggacagc ctggtgaact acctcggcaa gtttgcgcag
     2941 gattttctca gctcggccgg cattgcctat cgctcggaaa tgccaattca actgccccac
     3001 tggccgctgg actcgcaagt ccggcataat ttgtttctgg ccttcaagga ggcgctgcat
     3061 aacgccgtga aacatgcggc ggcgaaggaa gtgcatgtat ccctgacgtt gcgcaaggag
     3121 gcgtttgtgc tgacggtccg ggataacggc tgcggtttta ggcgcgacga tttggcttcc
     3181 tccgccgacc ccgctttgtc gcggctcagc accggcaatg gtctggtgaa catgaaaaac
     3241 cggatggagg gaatcggcgg tcattgtgaa ttacagagca agccgggcgc ggggacggtc
     3301 gtcaggtttg tggtgcgcct gcggcatccg gaaaaatgaa tggcgggctg cggctggaac
     3361 cgacagaggt ttccgagccg ggacatggga gttataattc ccgatgaaga gttaattttc
     3421 cggatttcgc caagcgcgcc tgtacttaaa aatgaggaca tcgtttggcc ggatttcgta
     3481 ttataatctg cataactggt gaatacgctt attaaagttt ccattgtcga agatgaccat
     3541 gcggtcaggg aaattttcgc tgactgggtg ggtcacgcgg acggtttccg ctgcgtgagc
     3601 acctacgcca acggcgagcg ggctgtggcc gacctgcccc gcgtgcgctc aaacatcgtg
     3661 ctcatggaca tcaaccttcc gcaaatgaac ggcgtggagt gtgtgcgccg gctcaagcca
     3721 ttgctgccgg cgacgcagtt tgtcatggtc accgtttatg aagatgccga ccatattttc
     3781 gatgccttgg cggctggtgc cgtcggctat ttgctcaaac ggacgccacg gaagcaattg
     3841 ctggcggcac tccgcgaagt taatgcggga ggttctccga tgagcaccaa tatcgcccgc
     3901 atggttgtcg agtctttcgg ccagccgcgg caaattgttt ctcccgagat gcaggggctc
     3961 tcgccgcgcg aacaggaagt gcttgaatta ctggcgcgag gctatctcta caaggaaatt
     4021 gccgaagcgc tcaaaatcag ccccctgacg gtgccttcgt tcgtccggcg catctacgac
     4081 aaactccatg tccactcgcg ggcgcaggcg gttgccaagg tggcgcattg gacaacacga
     4141 tgagggcggg gccattgaaa acaggcagag ctactccccc ggaattttcg caaaacccgg
     4201 tgtaccatgt tttggggaca tgacggcggg cgcacgacgg gtatacttcg ctcatagtaa
     4261 ttgaagtgtg ttaaatcaaa gaaaactatg aaaaagatac tattgactct cgcataaata
     4321 agtaacacaa tgctgccgca tgagcagcaa aacc
//
LOCUS       DAOPQG010000170         4031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_24174_length_4031_cov_12.962274, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000170 DAOPQG010000000
VERSION     DAOPQG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24174_length_4031_cov_12.962274"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1018)
                     /locus_tag="PKI07_13315"
     CDS             complement(89..1018)
                     /locus_tag="PKI07_13315"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC52060.1"
                     /translation="MSRRRFPGWFLWGLLGLTVLPASALLGLYWYFHPAVHRSNGIIY
                     GQRGEHPLALDVITPARPNGLGVLVLVSGKWKSSAAGKFREWLAAPILRRGYTVFAIY
                     HVSQPQASVMEIIADMHRAVRFVRHHAREYGVDPQRLGVSGGSSGGHLSLMLATRGGP
                     GPRETPDEVDRESSSVQAVAVFFPVTDLLNQGASTENLHDGGPPRRFRRAFGPQGTNL
                     SAWKVIGREASPIYHITTNLPPVLIYHGDADTLVPLEQSLWFKARAREQGREVKLVVH
                     PGGRHGWTTMVFDTRRFADWFDQHLRPSSTAIP"
     gene            complement(1144..3483)
                     /locus_tag="PKI07_13320"
     CDS             complement(1144..3483)
                     /locus_tag="PKI07_13320"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOC52061.1"
                     /translation="MKRHTLSWLKILLAPTLAALAGAAAAEFDQARWIAPPAGMGTNQ
                     PCPRFRKEFILDRAPRRAWLRLAGLGDFDARVNGQRLADTGMNQPWSQYEKTLYYREF
                     DITARLTRGTNCVGVMLGNSFWNNPNPPPGRYNKHGPQRTASEPFLLRAEIRFEPRRG
                     TPGTLGSDETWRAADGPVVFSHIYAGEDFDARLQEPGWDSAGFDDHAWQAVRLAGAPT
                     GQLTPALWPGIKAFPPEPAASVRQPAPGVFLYSFPRNTAAQLRITVTGGKAGDRIQFR
                     CGEHKNAEDRLFGGYIVNSDLITDGQPHTHQWLFFYLGMQFVEVTGAVPEGEANPGNL
                     PVIRRLELVPVRAGLAETGSFHCASDLYNGTHQLIDAAMRANMSWVLTDCPHREKLGW
                     LECAYLLEPSFLYRYEGRDWFRKIAHDIRDAQEPSGRVLTVAPSYPAGRFPGAFNWTV
                     EWGAAAVLVPWQHYQWFGDRQILKDHFDLMRRFTDFIGREARDGLAPAGLGDWYDYGH
                     GQPPGPSRFTPTELSATATWGLCAEAVSRAAQALGRPRDARAYRELHTQIAADFRRHF
                     QDPATRKLRHHGSPQCANAMALCASLVPPEDRALLVADIIADLKQRDWQQTPGDVGHA
                     YFIRALAEAGRSDVLHRVYSRTGLGSYGGILAKGLTALPETWDAMMDGCQSLNHCMLG
                     HVMEWYYGYVGGIRQPPDGIGWREILIAPNPGPLASAATLVRTPRGPVASRWRNDNGS
                     FRLEVEVPHGVKATAILPSGARQKLHGGKQTLQEPLPGP"
BASE COUNT          644 a         1298 c         1430 g          659 t
ORIGIN      
        1 cccgcttaca gcctcccaac gggctaagcg ggcaagatgc ccgcgctccg gcctgtgggt
       61 atttcgtgcg gggcgcgaaa tatcccggct aggggatggc tgtggatgag ggccgcaagt
      121 gctgatcaaa ccagtccgcg aatctgcggg tgtcgaacac catcgtcgtc cagccgtgcc
      181 tgccgcccgg atgcaccacg agcttcacct cgcggccctg ttcgcgcgct ctggccttga
      241 accacaggga ttgctccaag ggcaccagcg tgtccgcgtc gccgtgatag attagcaccg
      301 gggggagatt ggtcgtgatg tggtagatgg gagaagcctc gcggccgatg actttccatg
      361 ccgatagatt ggttccctgg ggaccgaacg cgcggcggaa acgccggggc gggccgccgt
      421 cgtggagatt ttcggtggat gcgccctggt tcaacaggtc ggtcacgggg aagaagacgg
      481 ccaccgcctg caccgagctg ctctcgcggt ccacttcgtc cggcgtttcc cgcgggccgg
      541 gcccgccgcg ggtcgccagc atcaggctca ggtgcccgcc ggagctgccg ccggataccc
      601 ccagccgctg cgggtccacg ccatattccc gcgcatggtg ccggacgaag cgcacggccc
      661 ggtgcatgtc cgcgatgatt tccatcaccg aagcctgcgg ctgtgataca tggtaaatgg
      721 cgaatacggt gtagccgcgc cgcaggatgg gcgccgccag ccattcgcgg aatttgccgg
      781 ccgcgctgga tttccatttc ccgctgacca gcacgaggac gcccaggccg ttggggcgcg
      841 ccggggtgat tacgtccagg gccagcggat gctcgccgcg ctggccgtag atgatgccat
      901 tgctgcgatg cacggccggg tggaaatacc aatagagccc gaggagcgcc gaggccggca
      961 acaccgtcag gcccaacagc ccccacagga accagccagg aaaccggcgg cgagacatgg
     1021 ggcaacaata cgctggaggc aggcttttga ccacaacccc ctttagccgg ctttcacagc
     1081 tctgcctggc agggggccgg ttgcctgcgg cgctccgcgg ttatgttggc cggctgagga
     1141 tgcttacggg ccgggcaggg gttcctgaag ggtttgcttg ccgccatgca gtttctggcg
     1201 cgccccggag gggagaatgg ccgttgcctt cacgccatgg gggacttcga cttcgagccg
     1261 gaaggagccg ttgtcgttgc gccagcggct ggcgacgggg ccgcgcggcg tccgcacgag
     1321 ggtcgccgcg ctggccaggg ggccggggtt gggcgcaatg aggatttccc gccagccgat
     1381 gccgtcgggc ggctgccgaa tgccgccgac gtagccataa taccactcca tcacgtggcc
     1441 cagcatgcaa tggttgagag actggcagcc gtccatcatg gcatcccagg tctcgggcag
     1501 cgcggtcagc cccttggcga gaatgccgcc gtagctgccc aggccggtgc gcgaatagac
     1561 gcggtgcagg acatccgaac ggcccgcctc cgccagggcg cggatgaaat aggcgtggcc
     1621 cacgtctccg ggcgtctgct gccagtcccg ttgcttcaga tcggcgatga tgtccgcgac
     1681 cagcagcgcg cggtcttccg gcgggaccag gctggcgcat aaggccatgg cgttggcgca
     1741 ttgcgggctg ccgtggtggc ggagtttgcg ggtggccggg tcctgaaaat gccgccggaa
     1801 gtcggcggcg atttgcgtgt gcagctcgcg gtaggcgcgg gcgtcccggg gacgccccag
     1861 cgcctgggct gcgcgggaga cggcttcggc gcacagtccc caggtggcgg tggcggacag
     1921 ctcggtgggc gtgaagcgcg acggacccgg cggctggccg tggccgtaat cataccaatc
     1981 ccccagcccg gcgggggcga ggccatcccg ggcctcccgc ccgatgaaat cggtgaagcg
     2041 gcgcatcagg tcgaagtggt ctttcaggat ttgccggtcg ccgaaccact ggtagtgctg
     2101 ccagggcacc agaaccgccg ccgcgcccca ttccaccgtc cagttgaacg ccccggggaa
     2161 gcggcccgcc gggtagctgg gagccacggt caggacgcgc ccgctgggct cctgcgcgtc
     2221 gcggatgtca tgggcgatct tgcggaacca gtcccggccc tcgtagcggt agaggaagct
     2281 gggttccaac aagtaggcgc actcgagcca gccgagcttt tcccggtgcg gacaatcggt
     2341 cagcacccag ctcatattgg cgcgcatcgc cgcgtcaatg agctggtgcg tgccgttgta
     2401 gagatcgctg gcgcagtgaa agctcccggt ctcagccagc ccggcgcgca ccggcacgag
     2461 ctccaggcgg cgaatgaccg gcagattccc agggttggcc tcgccctccg gcacggcgcc
     2521 cgtgacttcg acgaactgca tgcccaggta gaagaagagc cattgatgcg tgtggggctg
     2581 gccgtcggtg ataaggtcgc tgttcacgat atagcccccg aacagccggt cttcggcatt
     2641 tttgtgttcg ccgcagcgga attggatgcg gtcgcccgcc ttgccgccgg tcaccgtgat
     2701 tcgcaactgg gcggcggtat tccgggggaa agaatagagg aaaacgcccg gggctggttg
     2761 cctcaccgag gccgcgggtt caggcgggaa cgccttgatg cccggccaga gggcgggcgt
     2821 gagctggccg gtgggggcgc cggccagccg gacggcttgc caggcatgat catcaaagcc
     2881 cgcggaatcc catcccggct cctggaggcg cgcgtcgaag tcttcccccg cgtagatgtg
     2941 ggagaacacc accgggccgt ccgccgcgcg ccaggtttca tccgagccga gagtgccggg
     3001 cgtgccgcgc cggggctcga aacgaatttc ggcgcgcagc aggaagggtt cactggccgt
     3061 gcgctgcggg ccgtgcttgt tgtagcgccc cgggggcggg ttgggattgt tccagaacga
     3121 gttgccgagc atgactccca cgcaattggt tccgcgggtc agccgggcgg tgatgtcgaa
     3181 ctcgcgatag taaagggtct tctcgtattg cgaccagggc tggttcatgc ccgtgtcggc
     3241 gagccgctgg ccgttgacgc gcgcgtcaaa gtcccccagc cccgcaaggc gcagccaggc
     3301 gcggcgcggc gcccggtcga ggataaactc cttgcggaac cgcgggcagg gctggttggt
     3361 gcccatcccc gccggagggg caatccagcg cgcctgatca aactccgccg ccgcggcgcc
     3421 ggccaaggcg gcgagcgtcg gcgcaaggag gattttcagc caactgagtg tgtgtcgttt
     3481 cataatcgcc gggttcattc ggcgggcagc gaggaatgac ccgccccggg cggggttgcg
     3541 tcgtgccctg gttgccgaag cggatgccag cccggctgaa atcatcaccg cttcggtcgc
     3601 cccgcgatgg cggcccgccg ctgaactaaa tgccgccaca gcgtaccccc acccctcgca
     3661 ggcgcaactc aaaactggaa gaaaaagccc gtggtggcgc atcccggctc cgcgccgaat
     3721 cgaaggcgga gtgcgggccg cgccggggcc agcgattgcg gcaccgggtg ttttccggtg
     3781 gggcaaggat tcatccccgc cgggtccggg gctgacctgg atgattacgg cggcattggc
     3841 ccgggcggca gggcgctttc tcccgaccgc agccaggcgg ctcgcggcgg gcgcgctgat
     3901 agcgaccgtg aatgcgggcc ggggattccc gtttggcgga tagactcatg ttcgtggctt
     3961 cggtaaccat gaacttgagc caacgtatcg gcgaatgcct cgtccacctc cccgccccgg
     4021 caacatttat t
//
LOCUS       DAOPQG010000171         3998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_24426_length_3998_cov_14.888410, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000171 DAOPQG010000000
VERSION     DAOPQG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24426_length_3998_cov_14.888410"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..400
                     /locus_tag="PKI07_13325"
     CDS             68..400
                     /locus_tag="PKI07_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52062.1"
                     /translation="MMKRRNDEPQEWWKSLSDGERRLIWNVMKMLRGAKRSAILDVAR
                     AAAKWESGIGCFIKFRLMNSWRTTQRRRVIDSGWVKVEGRRQNGAGRAISSPSNGHSQ
                     PGAAANRD"
     gene            453..1070
                     /locus_tag="PKI07_13330"
     CDS             453..1070
                     /locus_tag="PKI07_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52063.1"
                     /translation="MNLRQFDLVLLGLDPVLNADFDQVCGGCACGACHLAGIHAAGGN
                     KLSLGVKIGGKHFKDGGHILIGQVAGLAANDAADEAAGKAALASDIALAQVMAFGLAL
                     ECDTEVAHAYVDGRWFTIGEPTPAQIFFVPCVATYATGQQMQQFPVEGFGRVFESRAK
                     RNPVNGYVRKSCDEGLSRAGKSCALVRVGAWRDLGHGEELRKHAL"
     gene            1118..1360
                     /locus_tag="PKI07_13335"
     CDS             1118..1360
                     /locus_tag="PKI07_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52064.1"
                     /translation="MKIEQKYSQSAEQSRVEEYIDKREVGKRLRMQPRTVDAWMRRGL
                     LPYYKLGRVVRFKWSEVECHLSERCRVVRELTKAGL"
     gene            1357..1764
                     /locus_tag="PKI07_13340"
     CDS             1357..1764
                     /locus_tag="PKI07_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52065.1"
                     /translation="MMNAREKGKRGERQWRDELRSKGYAARRGQQFCGSPESPDVVCD
                     GLPWAHFEVKAVERLNIEDAMEQARRDARGKVPVVAHKRSFCKWLVTMEADAFFRLIR
                     GDFTTEAPRHRGANGTNGTNGADVSRGRGEFEI"
     gene            1860..2453
                     /locus_tag="PKI07_13345"
     CDS             1860..2453
                     /locus_tag="PKI07_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52066.1"
                     /translation="MPHPEGIHPAVCVDVIDLGLVETEFQGQRRLVNKVRLVFESEQK
                     NEEGKNCIITKTFTASLHSKAKLAEFLGKWRGRPVVPGESIDLGKLMGANCTLVVSHQ
                     QNMVGRTYASIDAVSKPTKRLVASGWYDPAAARQRIAERKAKEQAGSGGYPQTNVAAS
                     RPLNSVGAAFPQPARAQPSAAPQQAPAPASNEDDVPF"
     gene            2489..3304
                     /locus_tag="PKI07_13350"
     CDS             2489..3304
                     /locus_tag="PKI07_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52067.1"
                     /translation="MKLFNPDNAHWYKRDGEPLHSVLSGRGEPRPTTLRDARKLGLLP
                     SVTNILGVINKPELVEWKMTQAVLAALTLPRLEGEGEDAFARRVVDDAQSRVRTAAEF
                     GSVFHAGAEHVAKTLEVDPAGPYAAWLNLHRDWFQGICVRVLWTERVLVNTELGYAGT
                     ADLLMEHRQHGLTLVDYKTQGVKPGNTARAYGSWCQQLAAYRRAIGAPMSCLSVIVNS
                     AEPATPVEHLWSEVELRAGLESFEAALVIWRNEKGYDPRVAPAPGALPVLTAA"
     gene            complement(3712..>3998)
                     /locus_tag="PKI07_13355"
     CDS             complement(3712..>3998)
                     /locus_tag="PKI07_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52068.1"
                     /translation="TMNQNSHSNWINKWGLATRAPAEPADLGLPFNGTEQPFRCLSWV
                     LHAIISKRIIRQRSRNHQSRLIIPLARVADVAQHLQISLPELPSVEAHPL"
BASE COUNT          848 a         1016 c         1395 g          739 t
ORIGIN      
        1 taagtttcgc gggggatttg tacgggcggg aacgcatttg tcccgccccc ctgtgtagtg
       61 ttggcgcatg atgaaacgca gaaacgatga gccccaggaa tggtggaaga gcctttcgga
      121 tggcgagcgg cgattgattt ggaatgtgat gaagatgctg cgcggagcca agcgaagcgc
      181 gattctggat gtggcgcggg cggcggcgaa gtgggaatca gggattggct gcttcatcaa
      241 gttccgcctg atgaactcat ggcggaccac gcaacgccgg cgggtgatcg acagcggatg
      301 ggtgaaagtg gaaggccgcc gacagaacgg ggctggacgc gctatttcat ccccgtcaaa
      361 cgggcatagc caaccaggtg ctgctgcaaa tcgggattga tggggcactg gcttctcaac
      421 cacgcgaaag ctttttctgt ttctgaactc aaatgaactt gcggcaattc gacctcgttc
      481 ttctcgggct tgaccccgtg cttaatgctg atttcgacca ggtctgcggc gggtgtgcct
      541 gcggcgcgtg ccacctcgcg ggcatccatg cagcgggcgg caataagctc agcctgggtg
      601 tgaagatcgg cggcaaacac tttaaggatg gcggccacat tttgatcgga caagtcgcgg
      661 gactcgccgc taatgacgcg gctgatgaag ctgcgggaaa ggccgcactg gcgagcgaca
      721 tcgctctggc tcaagtgatg gcgttcggcc tggcgctgga gtgcgacacc gaagtagctc
      781 atgcgtatgt tgacgggcgt tggttcacga tcggggagcc aacacccgcg cagatttttt
      841 ttgtcccatg tgttgcaaca tacgcaacag gacaacaaat gcaacaattt cccgtggagg
      901 gttttggccg tgtgtttgaa tcgagggcga agcgaaaccc tgtcaacggg tatgtgcgga
      961 aatcttgtga tgaaggcctg tcgcgagcgg gtaagtcgtg cgcgttggtg cgcgttggtg
     1021 cgtggcgcga tttgggccat ggggaagaac tccgaaaaca tgccttataa cagtcacgcg
     1081 agcgcgtgtt cgaacgcgag ccggaaatga gaagcgtatg aagatagaac agaaatacag
     1141 ccagagcgcg gagcagagcc gtgtcgagga gtacatcgac aagcgcgaag tgggcaagcg
     1201 gttgcggatg caaccgcgca cggtggatgc ctggatgcgg cgcggcctgc tgccgtacta
     1261 caagctgggc cgggtggtgc ggttcaagtg gagcgaagtg gaatgccatc tctccgagcg
     1321 gtgccgcgtg gtgcgcgaac tgaccaaggc ggggctgtga tgaacgcgcg ggaaaaaggc
     1381 aaacgcggcg agcggcaatg gcgcgatgaa ctgcggagca aaggctacgc cgcgcggcgc
     1441 gggcagcagt tctgcggatc gccggagtct cccgacgtgg tgtgtgacgg cctgccgtgg
     1501 gcgcatttcg aggtgaaagc ggtcgagcgt ctgaacatcg aggacgcgat ggagcaggcg
     1561 cggagagacg cgcgcgggaa ggtgccggta gtggcgcaca agcgcagctt ctgcaaatgg
     1621 ctggtgacga tggaggccga tgcgttcttc cgtttaatca gaggggattt caccacggag
     1681 gcaccgaggc accgaggggc gaatgggacc aatggaacga atggggcgga tgtgtcgagg
     1741 ggccggggag aatttgaaat ttgagatttg aaaggcccgg ttgggcctgg agagaaaaac
     1801 aaaaaaacaa agaaaggaaa gtaagtatgg ttctgcaatc aaaaatcggt ggcgattata
     1861 tgccgcatcc tgaagggatt catcccgcag tgtgcgtgga tgtgattgac ctgggactgg
     1921 tggaaaccga attccagggg cagcggcggc tggtaaacaa ggtgcggctg gtgtttgaat
     1981 ccgaacagaa gaacgaggag ggcaagaatt gcatcatcac caagacgttc accgccagcc
     2041 tgcacagcaa agcgaagctg gcggagttcc tgggcaagtg gcgggggcgg ccggtggtgc
     2101 cgggggagag catcgacctg ggcaaactga tgggcgcgaa ctgcaccctt gttgtgagtc
     2161 atcagcaaaa catggtgggt aggacgtatg cctccattga cgcggtgagc aagcctacca
     2221 agcggctggt ggcctcgggg tggtatgacc ctgccgcggc tcggcaacgg attgcggagc
     2281 gcaaggcgaa ggaacaagcc ggctctggcg gctacccgca aacgaatgtg gccgcgagcc
     2341 gcccgctgaa ttctgtggga gcggccttcc cgcaacctgc gcgggcgcaa ccgagcgccg
     2401 cgccccagca agcgccggca ccggcgagca acgaggacga tgtgccgttt tgatctgcaa
     2461 cacgcaaccg ccaagcactg attgaatcat gaaactattc aaccctgaca atgcacattg
     2521 gtataagcgg gacggagagc ccttgcattc cgtcctgtcg ggccggggag agcctcgccc
     2581 gacaaccttg cgggatgctc gcaagctggg cctgctgccg agcgtgacga acatccttgg
     2641 ggtcatcaat aagcccgaat tggtggaatg gaagatgacg caagcggtgc tggcggcatt
     2701 gacactgccg cgcctcgagg gcgagggcga ggatgcgttt gcgcgtcgtg tggtggacga
     2761 cgcgcaaagc cgcgtgagga cggcggcgga gttcggctcg gtgttccacg ccggggccga
     2821 acacgtggcc aagactttgg aggttgatcc tgccggtccg tatgccgcct ggctcaacct
     2881 ccaccgcgat tggtttcaag gtatctgcgt gcgtgtgctc tggactgagc gggtgctggt
     2941 aaacaccgag cttgggtatg ccggcaccgc cgacctgctg atggaacatc gacagcacgg
     3001 cttgaccctc gtggactaca aaacccaggg cgtcaaaccg ggaaatacgg cccgagccta
     3061 tggctcgtgg tgccaacaat tggcggcgta tcggcgggcc atcggcgcgc cgatgtcctg
     3121 cctgagcgtc attgtgaact cggccgagcc tgcgacacct gtggaacacc tatggagcga
     3181 ggttgaactg cgagcgggac tggagtcctt cgaagcggct ctggtgatct ggcggaacga
     3241 aaaagggtat gacccacgcg ttgcgccggc gcctggagcg ctgccggtcc tgacagcggc
     3301 atgaaaggaa cgaatgaaca cctgggcgga agggggggcg atgcgaagcc ggcttcgccc
     3361 ccgaccgcca ccgtgcagcg gcttgaacgc ctccttgggg gcgacccggc gactgaggct
     3421 cagattcttc agttcatagc cgcgcatttc ggagcggtct ccttgtgcga cttaccgccg
     3481 cagattgcgg cggaaatttg ccggaggcct gcggatttta ttcgggcggc aaagaacttc
     3541 tgccagccgg agttgttctg aatgagccga gcggcaaaga actgggcgag gcggaagcgc
     3601 cgggaactga tggatttgct tgggaatgtg tgcgcgcatt gcggaacggg agagaatctt
     3661 gagttcgatt gtatttatcc gcagggggac gggcatcacc ggggcgaagc atcacagcgg
     3721 atgtgcttct accgaaggca attccgggag ggaaatttgc aggtgctgtg caacgtctgc
     3781 aacgcgcgca agggggatga tgagccggct ttggtggtta cggctccgct gccggataat
     3841 cctttttgaa atgatggcat gcaggaccca cgacagacag cggaacggct gctcggtgcc
     3901 attgaatggc agaccgaggt cagcgggttc tgccggtgcc cgggtggcaa gcccccattt
     3961 attgatccag ttgctatgag agttttggtt catggttt
//
LOCUS       DAOPQG010000172         3932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_24969_length_3932_cov_14.644571, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000172 DAOPQG010000000
VERSION     DAOPQG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24969_length_3932_cov_14.644571"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1443
                     /gene="sppA"
                     /locus_tag="PKI07_13360"
     CDS             325..1443
                     /gene="sppA"
                     /locus_tag="PKI07_13360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOC52069.1"
                     /translation="MDEHVPSSPIPPPPVIGSPPPAPPRRRSRGWMILALVLLALLAI
                     SVLFNLGQFLAGVMPVTVAGSPAGGPRLDEVIKEDNKASSKIALIEVDGVITSQALDQ
                     AGHSMVQVIKAQLKRAEQDDKVKAVILQVDSPGGEVLASDEIARAIAEFQDRPKGKPV
                     VCSMGRLAASGGYYVSAPCRWIVANDLTITGSIGVILSTWNYRGLMDKVGLQPETFKS
                     GRHKDMLSGSREPGSMTQEERDMVQALINETYAKFTNVVATGRELARGRNQGEGRALS
                     KNWPQHADGRILSGTEAFKLGFVDELGTLEDAVRRAKSLAGIRNANLIKYQRRYDLSD
                     LFQFFGQTESKVVKVDLGLEMPKLQAGQLYFLPSMFLR"
     gene            1447..2070
                     /gene="upp"
                     /locus_tag="PKI07_13365"
     CDS             1447..2070
                     /gene="upp"
                     /locus_tag="PKI07_13365"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOC52070.1"
                     /translation="MKGVTVITHPLVRHNLTRLRDERTGPQEFRRVLSEVAALMVYEA
                     TRSFAVAPLRVKTPLSRAAGHRLQREVVLVPVLRAGLGMLDSILQLIPHARVGFIGLK
                     REETTLRATSYHQSLPPNLKPFEVILIEPMLATGGSAVAALDLLAELGARHVRVVNLV
                     AAPEGIRRVRAHYPKLPIFTAAVDRRLNRKGYILPGLGDAGDRLFGV"
     gene            2072..3205
                     /locus_tag="PKI07_13370"
     CDS             2072..3205
                     /locus_tag="PKI07_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOC52071.1"
                     /translation="MRNRETLLMVADSERDANMLYAVGMFVPDPFIWLRHRGRDYVLL
                     SDLEFDRARRRAPHCRVLSLSRYQAKLRSRGIQRPELAGIILLLLRERGIRRVVVPQN
                     FPHGLAAELLRRRIAVKTRPGSFFPERECKSAAEVRKIRAALLMAEIGMAAAMQALRR
                     SKIGKDRRLVYRALPLTSERLRAIIDAAILQAGGLAQHTIVAGGKQGCDPHETGYGPL
                     RAHEPIILDIFPRSQKTGYFGDITRTVVRGRATEAIRKLYDTVLRGQKLAFQKLRPGT
                     PTAQVHTIVQTFFQNEGYPTGRRNGRMEGFFHGTGHGLGLEIHEAPRVGSASAGALQP
                     GQVVTVEPGLYYPELGGVRLEDVALITRRQPRNLTRFEKVLEV"
     gene            3281..>3932
                     /locus_tag="PKI07_13375"
     CDS             3281..>3932
                     /locus_tag="PKI07_13375"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOC52072.1"
                     /translation="MKRATKAPTDPELQRRIQALIDYKGGGHNEESVADLVENALKLL
                     KDVEHTGDVRVIQTALRELRYAFKLFAPYAHVRKVTMFGSARTQPTRQEYQQGVEFGR
                     KIAAAGFMVITGAGPGIMQAGHEGAGPEKSFGVNIRLPWEQSANPVIREDKKLVTFKY
                     FFTRKLIFIRHADAIALFPGGFGTMDEGYEALTLMQTGKSQLMPLVLIDRPGGTFWK"
BASE COUNT          726 a         1246 c         1259 g          701 t
ORIGIN      
        1 ctggccagcc tcggaaagca gcatcgctat cgcaaggcag ccaaggccgg acaggatggc
       61 ccgttcaaaa gtgggttaac aggaggggtt gtttgactgt gcccggcttg aaaacgggag
      121 aaaaggggca tgcgcgggag ataaggttca gtttgccact gccggagcct gtctttaagc
      181 ggaaaatctc ctgccccccg gggctcaggc acccctggct tccgcgcctt tattcgcatc
      241 ggaatccagc ccgcgaaagc agcccggccg gcgattctaa tccgcttgcc catccttcct
      301 gaattgcact agtctgcgcc tcgtatggat gaacatgtac cgtcatcgcc gattccaccg
      361 ccgccggtca tcggatctcc cccccctgcc ccgccccgcc gccgcagccg cggctggatg
      421 atccttgccc tggtgttgct ggcactgctg gcaatcagcg tgctcttcaa cctcgggcag
      481 ttcctggccg gggttatgcc ggtaaccgtc gccggttcgc cggccggcgg cccgcgcctc
      541 gacgaggtca tcaaggaaga caataaagct tccagcaaaa ttgccctgat tgaggttgac
      601 ggcgtcatta ccagccaggc gctggaccag gccggccaca gcatggtgca ggtgatcaag
      661 gcgcaactga agcgcgcgga gcaggatgac aaggtcaagg cggtgattct ccaagtggat
      721 tctccgggcg gagaagtcct ggcttcggac gaaattgccc gggccatcgc cgagtttcag
      781 gaccggccga aggggaaacc cgtagtgtgc tcgatggggc ggctggctgc gtccggcggg
      841 tactatgtat ccgctccctg ccgttggatc gtggcgaacg atctgacgat caccggcagc
      901 atcggggtaa ttctgagcac ctggaattac cggggcctga tggacaaggt cgggctgcag
      961 ccggagacct tcaagagcgg caggcataag gacatgctga gcggttcgcg cgagccgggg
     1021 tcaatgacgc aggaagaacg ggacatggtg caggcgctca tcaatgagac ctatgccaaa
     1081 ttcacaaacg tggtggcaac cggccgcgag ctggcacgcg gcaggaacca gggggagggc
     1141 cgcgccttaa gcaaaaattg gccccagcac gcggacggcc gcatactctc cggcaccgaa
     1201 gctttcaagc tggggtttgt ggatgaactg ggcacgctgg aggatgccgt tcggcgggcg
     1261 aagtccctgg ccgggattcg caacgccaac ctcattaaat atcagcggcg ctacgatttg
     1321 tccgacctgt tccagttttt tggccagacc gaaagcaagg tcgtgaaggt ggatttgggt
     1381 ctggaaatgc ccaagctcca ggccggccag ctttacttcc tcccgtccat gttcctgcgc
     1441 taacgggtga aaggggtcac tgtcatcacg cacccgctgg tgcgccacaa cctgacccgt
     1501 ctgcgcgacg aacgcaccgg gccgcaggaa tttcgccgcg tgctcagcga ggtcgccgct
     1561 ttgatggtgt acgaggcgac ccgctcattc gcggtcgcgc cgctccgggt caagacgccc
     1621 ttgtcccggg cggccggcca tcgcctgcag cgcgaggtgg tgctggtgcc ggttttgcgg
     1681 gccgggttgg ggatgctgga ttcgattctc cagctcatcc cgcacgcgcg cgtgggcttt
     1741 atcgggctga aacgggagga gaccacactg cgggcgactt cctaccatca aagcctgccg
     1801 ccgaacctga aaccctttga ggtaatcctg atcgaaccca tgctggccac cgggggcagc
     1861 gccgtggccg ccctggattt gctggccgag ttgggagccc ggcatgtgcg cgtagtcaac
     1921 ctggtcgccg cgccggaggg cattcggcgc gtgcgcgccc attatcccaa actgccgata
     1981 tttaccgctg ccgttgaccg gcgcctgaac cgaaagggct atattctccc cggcctggga
     2041 gatgccggcg accggctctt tggcgtttga catgaggaac cgcgaaaccc tcctgatggt
     2101 ggcggacagc gagcgcgacg cgaatatgct gtatgccgtg ggcatgttcg tgccggaccc
     2161 gttcatctgg ctgcgccacc ggggccggga ttacgtgctg ctgagcgatt tggagttcga
     2221 ccgcgcccgc cggcgggccc cgcactgccg cgttttgtcc ctcagccgct accaggcgaa
     2281 actgcgaagc cggggaatcc agcggccgga attggccggg attatcctcc tcctgttgcg
     2341 cgaacggggt atccggcgcg tcgtggtgcc gcaaaacttt ccgcacggcc tggccgccga
     2401 actgctgcgc cgccgcattg ccgtcaagac ccgccccggc agcttctttc ccgagcggga
     2461 atgcaagtca gccgccgagg tcaggaagat ccgcgccgcc ctgttgatgg cggaaatcgg
     2521 catggccgcg gcgatgcagg cgctgcgccg atcgaagatt ggcaaggacc gccgattggt
     2581 atatcgcgct ctgccgttaa cgtcggagcg gcttcgcgcc atcattgacg ccgccattct
     2641 tcaagcgggc ggcctggcgc agcacaccat cgttgcggga ggcaaacagg gctgcgaccc
     2701 gcatgaaacc ggctacgggc ctttgcgcgc gcatgagccc ataatcctgg acatcttccc
     2761 gcgctcgcaa aagactggct atttcggcga catcacccgc accgtcgtcc gcggccgggc
     2821 caccgaagcg attcggaagc tctacgatac ggtgcttcgg ggccagaaac tggccttcca
     2881 gaagctgcgc cctggcacgc cgaccgccca ggtgcatacg attgtccaga cattcttcca
     2941 gaacgaaggc tacccaaccg gcaggcgcaa cggccgcatg gaagggttct ttcatggcac
     3001 cggccacggg ctgggcctgg aaatccacga agcgccgcgc gtcggctcgg cctcggcggg
     3061 cgccttgcag ccgggccagg tggtcaccgt cgaacccggg ctttattatc ccgaactcgg
     3121 cggcgtccgg ctggaggatg tcgccctcat cacgcggcgg cagccgcgca acctgacgcg
     3181 gttcgagaag gttctggagg tttgagcggc ggggggcgcg ccctcgcccg ccacggcagc
     3241 cggcagttgc tttcgcccgg ttttgctcta tgctcggctc atgaaaagag caacgaaggc
     3301 acccaccgac ccggaactgc aacggcgcat ccaggcgctg attgactaca aaggcggcgg
     3361 gcacaacgaa gagagcgttg ccgacctcgt cgaaaatgcg ctcaagctgt taaaggacgt
     3421 ggagcatacc ggcgacgtgc gcgtcatcca gacggcgctg cgcgagttgc gttatgcctt
     3481 caagctcttc gccccctacg cgcatgtgcg caaggtcacc atgtttggct ccgcccggac
     3541 ccagcccacg cggcaggaat atcagcaggg agtcgagttt ggccggaaga ttgcggcagc
     3601 gggttttatg gtcattaccg gcgccggccc gggcatcatg caggccggcc atgagggcgc
     3661 cggcccggaa aagagcttcg gcgtcaacat ccgcctgccc tgggagcaaa gcgccaaccc
     3721 cgtcattcgc gaagataaaa aactggtcac cttcaagtat ttcttcaccc gcaagctgat
     3781 cttcatccgc cacgcggatg ccatcgccct gttcccgggc ggcttcggca cgatggacga
     3841 gggctatgaa gccctgaccc tcatgcagac gggcaaaagc cagttgatgc cgctggtgct
     3901 gattgaccgc cccggcggca cgttctggaa ga
//
LOCUS       DAOPQG010000173         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_25342_length_3887_cov_16.054280, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000173 DAOPQG010000000
VERSION     DAOPQG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25342_length_3887_cov_16.054280"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1325)
                     /locus_tag="PKI07_13380"
     CDS             complement(9..1325)
                     /locus_tag="PKI07_13380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC52073.1"
                     /translation="MNLVQLTPGAGAMFCGGCLRDNALVAALGQLGHTATLVPLYLPL
                     TLDEPDQSAGRPVFFSGLNVYLQQKSALFRHAPGWLHDWLASPRVLAWAAGRAAGTRA
                     ADLGALTLSMLRGEAGHQARELEELLAWLKSQPPPDVLCLSNALLVGMVRRLKSELRV
                     PLVCALQGEDSFLEALPVSHRAACWRVLAERAREVDRFIAPSRYFGDLMRARLGLGAE
                     QVRVVHNGITLDGYHKNGAGPGAGAGTAGAPVLGYFARMCPEKGLDTLVEAFICLRRQ
                     GGADRLKLRVGGSCGPADTPFVNRQRERLAASGLLGDAEFVPNPDRARKLEFLHSLSV
                     FAVPARYGEAFGLYILEALAAGVPVVQPRTAAFPELIAATGGGRLCAPEDPPALAAAI
                     GELLADPAQARALGQAGRRAVFERFSAETMARGVARVCGELIPGGA"
     gene            complement(1322..2431)
                     /locus_tag="PKI07_13385"
     CDS             complement(1322..2431)
                     /locus_tag="PKI07_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOC52074.1"
                     /translation="MRARVLWSGLLGLGCAVAAAPAADWPMFRGGPALTGVAGGRLPE
                     PLRLQWTFKTGGPVKSSAAIVGGRVFIGSDDGQVYALALADGRKVWAFKTGGEVESAP
                     LVLEGRVYVGSNDAFLYALEAATGKLVWKYETGDRIPGAPNWVKIGGETRVLVGSYDY
                     RLHCVAAATGRSNWVYATSNYINGAPAVADGQAVFGGCDGQLHVLSLAEGRPVREVEA
                     GAYIPASAALAGGRAYFGQYENEFLCVDLQAGRKVWSFHDRDFPYFSSPAVTREFVVF
                     GGRDKRLHCVRRADGQAVWSFATRGKVDSSPVVCGDRVVAGSGDGRLYLVSLDAGKAL
                     WVGEIGPPLESSPAVADGRVVIGAQDGGVYCFGAP"
     gene            2581..3159
                     /locus_tag="PKI07_13390"
     CDS             2581..3159
                     /locus_tag="PKI07_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOC52075.1"
                     /translation="MHRSPARLILLTGRRGAGKTRLLRRLVAQARQAGLTVFGLLAPA
                     QSRQGRKLRLDALAAHSGARRRLASRVAGELRGPRLGDWTFDSQTLAWGNALLARTPP
                     CGLFVLDELGPLEFEQHQGWNAAFALLDQPLACRLAFIVVRPECAAAFRRRYPQAKTL
                     TIRQPADRRRLARQLRETCRGGPPGTAAAGRL"
     gene            3394..>3887
                     /locus_tag="PKI07_13395"
     CDS             3394..>3887
                     /locus_tag="PKI07_13395"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52076.1"
                     /translation="MKARKSFMRFIHVNSSWGLLALALALAGPAQGADPILIGQWPGG
                     PRGWKAIGVAVDDHYAYVAGSDLSGLQVFDVSNPAHPRWLSDCITGESVFGVAVSGGY
                     AYAACYNAGLQVIDVSNPANPQWMGGYNTSGWAWDVAVSGDYAYVVNKSSTSADLQVI
                     NVSDP"
BASE COUNT          636 a         1451 c         1238 g          562 t
ORIGIN      
        1 gaggccggct atgccccgcc gggaatcaat tcgccgcaca cccgcgccac gccgcgcgcc
       61 attgtttccg cgctgaagcg ctcgaacacc gcccgccggc cggcttgccc cagggcccgc
      121 gcctgcgccg ggtccgccag caattcaccg atcgccgcgg ccagcgccgg cggatcctcc
      181 ggcgcgcaaa gccgtccgcc gcccgtggct gcaatcagct cggggaaagc cgccgtgcgc
      241 ggctgcacga ccggcacgcc cgccgccagg gcttcgagaa tataaagccc gaaggcttcg
      301 ccatagcgcg ccggcacggc aaagaccgag agcgaatgca gaaactccag cttgcgggcg
      361 cggtcggggt tggggacaaa ctccgcgtcg cccaacaggc cgctggccgc cagccgttcg
      421 cgctgccggt tcacgaacgg cgtatccgcc ggcccgcagc ttcctcccac gcgcagtttc
      481 agcctgtccg cgcccccctg ccgccgcagg caaatgaagg cttcgacgag cgtatccagt
      541 cctttctccg ggcacatccg cgcaaaatac cccagcaccg gcgcgcccgc cgtccccgcc
      601 ccggcccccg ggccggcgcc gtttttgtgg tagccgtcca gggtgatgcc gttgtgaacc
      661 acccgcacct gttccgcgcc caggccgagc cgcgcgcgca tcaggtcgcc aaaataccgg
      721 ctgggcgcga taaaccggtc cacctcccgc gcccgctccg ccaggacgcg ccagcacgcc
      781 gcgcggtgcg acacgggcag ggcttcgagg aacgagtctt ccccctgcag ggcgcagacc
      841 agggggacgc gcagttcgga tttgagccgg cggaccatgc caaccagcag cgcattggag
      901 aggcagagca cgtccggcgg cggctggctt ttgagccacg ccaggagttc ctccagttcc
      961 cgcgcctggt ggccggcttc gccgcgcagc atggaaagcg tcagcgcgcc caaatccgcg
     1021 gcgcgcgttc cggcggcccg gcccgccgcc cacgccagca cgcggggcga ggccagccag
     1081 tcatgcagcc agcccggggc gtggcggaac agcgcggatt tctgctgcaa gtaaacattg
     1141 agcccgctaa aaaacaccgg ccgcccggcg ctctggtccg gttcatccag cgtcagcggc
     1201 aggtagagcg gcaccagcgt ggccgtgtgg ccgagctggc ccagggcggc gaccagcgcg
     1261 ttgtcccgca ggcagccgcc gcaaaacatg gcgccggcgc ccggcgttag ttgcaccagg
     1321 ttcacggcgc gccgaagcaa tacaccccgc catcctgggc gccgatgacc acccgcccgt
     1381 ccgccaccgc cggcgaactc tccagcggcg gcccgatctc gccgacccac aacgccttgc
     1441 ccgcgtccag cgacaccagg tagagccgcc cgtcgcccga ccccgccacc acccggtcgc
     1501 cgcacaccac cggcgagctg tccaccttgc cccgcgtcgc aaagctccaa accgcctggc
     1561 cgtccgcgcg cctcacacaa tgcagccgct tgtcccggcc gccgaacacc acgaactccc
     1621 gcgtcaccgc cggcgaggag aaatacggga aatcccgatc gtggaaactc cacaccttcc
     1681 ggcccgcctg gaggtccacg cagagaaact cgttttcata ctggccgaaa tacgcgcgcc
     1741 cgccggccag cgccgccgac gcggggatgt aggcccccgc ctcgacctcc cgcaccggcc
     1801 ggccctccgc cagcgaaagg acatgcaact ggccgtcgca gccgccgaag acggcctggc
     1861 cgtccgccac cgccggcgcg ccgttgatgt agttgctggt ggcatagacc cagttgctgc
     1921 ggccggtcgc ggccgccacg caatgcagcc ggtaatcgta gctgcccacc agcacccgcg
     1981 tttccccgcc gatcttcacc cagttgggcg cgccgggaat ccggtcgccc gtctcgtatt
     2041 tccacaccag cttgccggtc gccgcctcga gcgcatagag aaacgcatcg ttcgacccca
     2101 catacacccg cccctccagg accagcggcg ccgactccac ctcgcccccg gtcttgaacg
     2161 cccacacttt ccggccgtcg gccagcgcca gcgcatacac ctgcccatca tccgagccga
     2221 taaacacccg cccgcccacg atggcggcgg aggatttgac cgggccgccg gtcttgaaag
     2281 tccactgcag ccgcagcggc tccggcaggc ggccgcccgc cacccccgtc aaggccggcc
     2341 cgccccgaaa catcggccaa tccgcggccg gggcggccgc caccgcgcac cccagcccca
     2401 gcagcccgct ccataaaact ctcgctcgca ttcggcccat aagcgtcaaa aaactcgcgc
     2461 tctgacgccc gattatcgtt cgcgcccgcg ccgccggcaa tcgaaaagcg ccgcccggcc
     2521 aataataccg ttgcgcgggg aggggggcgc ctgctattag cccgggacaa tccgcacgct
     2581 gtgcatcgct cacccgccag gttgattctg ctcaccggcc ggcgcggggc cggcaaaacc
     2641 cgcctgctgc ggcggctggt cgcgcaggcc aggcaagccg gcctgaccgt gttcggcttg
     2701 ctcgcgccgg cccagtcgcg ccagggccgc aagcttcggc tcgacgcgct ggccgcgcac
     2761 tcgggcgccc gccgccggct cgccagccgg gtggccggcg aactgcgggg gccgcgcctc
     2821 ggggattgga ctttcgactc ccaaacgctt gcctggggca acgcgctgct ggcccggact
     2881 ccgccctgcg gcctctttgt ccttgatgag ctgggcccgc ttgagtttga gcagcatcag
     2941 ggctggaacg ccgcgttcgc gctcctggat caacccctcg cctgccgcct ggccttcatc
     3001 gtcgtgcgcc cggaatgtgc ggcggctttt cggcgtcgct atccccaggc gaaaacgttg
     3061 acgattcgcc agcccgctga ccgccgccgg ctggcgcggc agctccggga aacttgccgg
     3121 ggcggcccgc cgggaaccgc cgccgctggc cgcctgtgat ttcttccccg gccggggcgc
     3181 gccgttcggt aaaattggct gttgacagat gcgcgcggtt tgtaataatc tcggctccaa
     3241 cacggggtaa gcaggagagc caaagggatt tgcccgcgcc cgggatgggc ggggtgacca
     3301 aaagcaggtg tggccgccgc gttatcccgc acgtcgtggg gcgcaaagga cgaaagcgtt
     3361 cgcgcggcag cgatagtcag gaaagaagtt attatgaaag ctcgcaagtc attcatgagg
     3421 ttcatccacg tcaattcgag ttgggggctg ctggcgctgg cgctggccct ggctggcccg
     3481 gcccaagggg cggatcccat tttaatcggt caatggccgg gtgggcctcg ggggtggaag
     3541 gccatagggg tggcagtgga tgaccactac gcctacgtgg ctggtagcga tttatccggc
     3601 ttgcaggtgt ttgacgtgag caacccagcc cacccgcgat ggctgagcga ttgcattact
     3661 ggcgagtctg tctttggcgt ggcggtcagt ggcggctacg cctacgcggc gtgttataat
     3721 gcgggtttgc aggtgattga cgtcagcaac ccggccaacc cgcaatggat gggcggctac
     3781 aacaccagcg gatgggcctg ggacgtggcg gtcagcggcg actacgccta cgtggtgaat
     3841 aaaagctcta ccagtgcgga tttgcaggtg attaacgtga gcgaccc
//
LOCUS       DAOPQG010000174         3654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_27528_length_3654_cov_14.914421, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000174 DAOPQG010000000
VERSION     DAOPQG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27528_length_3654_cov_14.914421"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKI07_13400"
     CDS             <1..372
                     /locus_tag="PKI07_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52077.1"
                     /translation="WRHNGVPLAGATASSYTRSNVEISDAGSYSVVITNVAGSVVSSN
                     AILEVHLPAPPQIDRIRLLPGGQIELRVSGAPGHYAVESSTNFVNWMELTNFVTSESV
                     FEYIEVETSLPQRFYRVKLIP"
     gene            583..3174
                     /locus_tag="PKI07_13405"
     CDS             583..3174
                     /locus_tag="PKI07_13405"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOC52078.1"
                     /translation="MNLIHPKTTILAAFLLFWFNGLTWADEWRAFWVDAWGPGHLRQS
                     EVDTLLGVPGTSKAGRIRDANCNAVFVQVRRNCDACYPSSMGEPYMSGLTPANFNALQ
                     AVINAAHDTTGGKKRIEVHCWMVAFRTSGGAVYKRHIDPPTGSLVHLDNYWPSRDADG
                     NEVDDKAFDPGHPLVLKYTVDVAMDLVSNFDLDGIHYDYIRFTAGNQGYNPTSIARYN
                     ARYGLTGQPSPADPQFQQWRRDQVSAVVRQVYARLQKAKPWVKQSGSFVTWNPSPVAS
                     TREAFQNTRPYYDVYCDWDSWMEEGIVDMAVPMTYYRQHQLPNDYLRWINFEKDRRFN
                     RHMIIGPGVYMNYLTNAVLHLQMTRTPSPQGNYADGFSGYSYRSPYLVKADPLTYGDW
                     SGFAPALVSNVTPTWAEIPAMPWKTAPTKGHLMGTVLLADSGAWADGAMVNISGPTNR
                     TMRADATGFYAFIDLPPGNYTVAASKPDYATASANVNIALGAVTGNMYEQNFSLIVPP
                     ASPVLVWGDNTFGQSAAPELATNAIAVAAGAWHTLALRADGRVVAWGDNSSGQCGDGE
                     PMTNALAIAAGGYHNLAIQRDGAVVAWGANDYGQASVPPGLSDVIAVAAGTWHSVALR
                     ANGTVVVWGDDSFGQKSRQPAGLTQVVAVAAGGNHTLALKADGRVVAWGENTDAEGNV
                     VGQSEVPWDLADVVAIGAGGYHSLAVKADGRVVGWGDNSQGQVSVPAGLSNVVAVAGG
                     GMHSVALCGDGSVVAWGANWSGQSGIPAWLPAAVGISAGEWHTVGLLEGTLPEARLHC
                     PARKGSRFRVLAQTMNRKTYVLERLDSLGETNWVGVSTNAGNGALRMLTDPAATAPQR
                     FYRMKQW"
BASE COUNT          727 a         1005 c         1166 g          756 t
ORIGIN      
        1 tggcggcata atggggttcc gctggcgggc gccacggcga gcagctacac ccggagcaat
       61 gtagagatca gtgacgcggg cagttattcg gtggtcatta ccaatgtggc cgggagcgtg
      121 gttagctcca atgccattct ggaggttcac ctgcctgctc cgccgcagat tgaccgtatt
      181 cgcctgctac ctggcggcca aatcgaactg cgggtcagtg gggcgccggg gcattacgcc
      241 gttgaaagct cgaccaactt cgtaaactgg atggagctga ccaatttcgt cacttcagaa
      301 agtgtcttcg aatatatcga ggtggagacc agcctgcctc agcgatttta ccgggtgaag
      361 ctgatccctt aaccagggtt gattgtttta ccaaggccac gctgccaggg gcgggatttg
      421 cagacgcccg cgcgggtgaa cgcaaaccgg tgcgactgcc gctccggtgc cggattgaca
      481 ccggtctcga aaagcgtaag atgggcgcat attagcagcc cgatcatgca caggcgcaac
      541 cgtcactgat tgtttcccgg ttttgattat ccgcttctac ctatgaactt gattcatcct
      601 aaaacgacaa tccttgccgc cttcctcttg ttttggttca acggcttgac ctgggctgac
      661 gagtggcgcg cgttctgggt ggacgcctgg gggccggggc acctgcgcca aagcgaggtg
      721 gacaccctgc tcggggtccc cggcacgagc aaagccggtc gaatccgtga tgccaactgc
      781 aacgccgtct ttgtgcaagt ccgtcggaat tgcgacgcct gctatccctc cagcatgggc
      841 gagccataca tgtccggcct tacaccggcc aactttaacg ccttgcaagc ggtcatcaat
      901 gccgcgcacg atactaccgg cggcaaaaaa cgcatcgagg ttcactgctg gatggtcgcc
      961 ttccgcacga gcggcggcgc agtgtataag cggcatattg atcctcccac cggcagttta
     1021 gtccaccttg acaactattg gccgagccgc gatgccgatg gcaatgaggt tgatgataag
     1081 gcctttgatc ccggccatcc gctggtcttg aaatataccg tggatgtggc catggattta
     1141 gtgagcaact tcgaccttga cggcatccat tacgactaca tccgcttcac cgccggcaac
     1201 cagggctaca acccaaccag catcgcgcgc tacaatgccc gttatggttt gaccggccag
     1261 ccgtcgcctg ccgatccgca attccagcag tggcggcgcg atcaagtcag cgcggtggtg
     1321 cgccaggtct atgcgcgcct ccaaaaagcc aaaccctggg tcaaacagtc cggctccttc
     1381 gtcacttgga atccctcgcc ggttgcctcc acccgggagg cttttcagaa cacccgtcct
     1441 tactatgacg tgtattgcga ttgggatagc tggatggaag aaggaatagt ggatatggcg
     1501 gtgccgatga cttactaccg ccagcaccaa ctccccaatg attaccttcg gtggataaac
     1561 ttcgagaaag accgccggtt caatcgccac atgatcatcg gccccggcgt ttatatgaat
     1621 tacctgacta atgccgtcct ccatttgcag atgacccgca cgccatctcc tcagggcaac
     1681 tatgccgatg gtttcagtgg ttactcttac cgcagcccct atttggtcaa ggcagacccg
     1741 ctgacttatg gggattggag cggctttgcc ccagcgttgg tctcaaacgt tactccaaca
     1801 tgggccgaaa tccccgcgat gccctggaag acagccccca ccaaaggcca tttaatgggc
     1861 acggtccttt tagccgactc gggagcctgg gcggacggcg ccatggtgaa catctccggg
     1921 ccgaccaacc gcacgatgcg cgcggacgcc acgggtttct atgcgtttat tgatttgccc
     1981 ccgggcaact acaccgttgc cgccagcaag cctgactatg cgaccgcatc ggcaaacgtc
     2041 aacattgccc ttggcgcggt taccggcaat atgtatgaac aaaacttctc tttaatcgtg
     2101 cctccggcct cgccggtttt ggtttggggc gacaatactt ttgggcaaag cgcggcaccg
     2161 gagttggcga cgaacgccat cgccgtggcg gcgggcgcct ggcatacgct ggccctgcga
     2221 gccgatggcc gggtggtggc ctggggagat aattcgagcg gtcagtgtgg ggatggagag
     2281 ccgatgacca atgccctggc cattgccgcc gggggttatc ataacctggc catacagagg
     2341 gacggagcgg tggtggcgtg gggggccaac gattacggcc aggccagcgt gccgccgggc
     2401 ctgagcgatg tgattgcggt ggctgccggg acgtggcaca gcgtggcctt gcgggccaac
     2461 ggcacggtgg tggtatgggg agatgacagt tttgggcaaa agagccggca accggccggg
     2521 ttgacgcagg tggtggcggt ggcggcggga ggcaatcata ccttggcctt gaaggcggac
     2581 gggcgggtgg tggcctgggg agagaacacg gatgcggaag ggaacgtggt ggggcaatcg
     2641 gaggtgcctt gggatttggc ggatgtggta gcgattgggg ctggggggta tcatagtctg
     2701 gcggtaaagg ccgacggccg ggtggtgggg tggggggaca attcgcaggg tcaagtcagt
     2761 gtgccggcgg ggttgagcaa cgtggtggcg gttgccggcg ggggcatgca tagcgtggcg
     2821 ctttgcgggg atgggagtgt ggtggcgtgg ggagcgaatt ggagtggcca gagcgggatc
     2881 cccgcgtggt tgcccgcggc ggtgggcata agcgccgggg aatggcacac ggtggggttg
     2941 ttggaaggca cgctgccgga agcgcggttg cattgcccgg cgcggaaagg gagtcgtttt
     3001 cgtgttttgg cgcagacgat gaaccggaag acctatgttt tggagcgatt ggatagtctt
     3061 ggggagacga actgggtggg ggtgagcacc aacgcgggta atggagcgtt acggatgctg
     3121 acggatccag cggccactgc gccccagcgc ttctatcgca tgaagcaatg gtagccgcac
     3181 gcgttttttg caataagcgc cccagttatg agggggcgca gttcggtttt aagacggtgc
     3241 acatcccgct ttaaatctcg atttataccc cgtttagccg ggccgattca gatttcgcct
     3301 ccaattcgac cccacgaaac gcgcaagttc ctggttttac gggtgaaaga cattcggaat
     3361 ccagcgaccc gcccgccgaa ttcgccagcc aaacggctcg ttgagactat ctgctggcct
     3421 gtcaggtcca cttcggcccc agtggaacga gggcgtacgg ttgaagtgag caacgtcaac
     3481 gtttcagaca aggccttctc gaagccaaaa gccatccggg cacccgcccg ccattgaaaa
     3541 gccgctattt gccagtcctg ggttcataac tgactgtttt tacggggctt aataatcatc
     3601 cggtagtgtc ctaaaagttc tgcaaaaagc agcgggtcat ggtaagccac tggg
//
LOCUS       DAOPQG010000175         3604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_28013_length_3604_cov_30.136095, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000175 DAOPQG010000000
VERSION     DAOPQG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28013_length_3604_cov_30.136095"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKI07_13410"
     CDS             <1..202
                     /locus_tag="PKI07_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008883249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52079.1"
                     /translation="ETRREHSSVVLTAPGQPFEQGIVLDAWRRSGRLYWSGVKEDKYP
                     WIEGRLITREPASSRASRKDAR"
     gene            802..1962
                     /locus_tag="PKI07_13415"
     CDS             802..1962
                     /locus_tag="PKI07_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52080.1"
                     /translation="MRVIHGQPSWRLASNEVEVYVTGTGGHLGPITFERKGRKLQPYS
                     VAPWATEKPACPMPPIIRVLRGDFFCLPFGNNTTPFRGEKHPVHGETANARWQFQTLE
                     TRRRRHLLHLSLRTKVRRGRVDKRIWLVDGQNVVYSQHVVSGLSGRTNLGHHAMLKFP
                     EAPGSGRLSTSRFVYGQVFPRAFELPEAGGYSWLKPGAAFDSLEEVPTITGRPADLTL
                     YPARRGFEDLVMLVNGDHVSFAWTAVTFPRERYVWFALKDPRILRETILWLSNGGRHY
                     PPWSGRHVNVMGIEEVTSYFHFGLAEAARKNPISARGFPTCLALSPTKPLVVPYIMGV
                     AAIPAGFDRVASIRAAQGNQAILLKSDSGRQVKAAVEVNFLRAGAGVGPGTG"
     gene            2040..3278
                     /locus_tag="PKI07_13420"
     CDS             2040..3278
                     /locus_tag="PKI07_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOC52081.1"
                     /translation="MRCTATSRLRGIIAGGGILFSIASALPAAEAGTAAKSQVRVDAG
                     FPGGNIVVDSIVGDTVTLHQDLRDTEGDWFYWHFRVRGAAGRTLQFCFSRSNPIGTRG
                     PALSLDGGKSWSWLGTNAVQGKTFTCAVPGGAREARFCFAIPYLESNLQDFLRRHRRH
                     RNLKVETLCQTPNGRRVELLRLGRLDGKAKQCVVLACRHHACEMIASYSLEGLMEAVL
                     DFEEVRPLREQVEFFVVPFMDKDGVEAGDQGKNRRPHDHNRDYHGQSLYASVRAVREL
                     ITGRLGSRNVALALDMHCPYIRGAHNESIYFVGGPEEQNWARVGEFARVLEAVQRGPL
                     HYRLADNLPYGRAWNTLEGPPRSFGRWTAAQPNIRVGTSIEIPYANVGETTVTAENAR
                     AFGHDLARALAQYLPLRIAD"
     gene            3316..>3604
                     /locus_tag="PKI07_13425"
     CDS             3316..>3604
                     /locus_tag="PKI07_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52082.1"
                     /translation="MRSCERQTSPGRLQAVPRPACAQLTFLFRLPIALGLVALCGLLV
                     SCGPKTEQPPAPPASQTNAAASAAALSPELAKLAGKWERPDGGYVLEIKGID"
BASE COUNT          647 a         1147 c         1175 g          635 t
ORIGIN      
        1 cgagacgcgg cgggaacaca gctcggtggt tctgaccgcc cccggccagc cgtttgagca
       61 gggcatcgtg ctcgatgcct ggcgccgttc cgggcgtctc tactggtcgg gcgtcaaaga
      121 agacaaatac ccctggatcg agggcaggct aatcaccagg gagccggctt catcccgggc
      181 gtcgcgcaaa gacgcacgct gagtctgaac ggggcgggat agtcgctcgc ctgcagcgtt
      241 ttgagtgggc gagacaaatt ccttctcgaa cgtggaattc tatcatgcac actatttcag
      301 cccccgcagt ctcctgccgt gagaggcggt atggatcgag cggaatggtc aggagccgct
      361 gcgatttccg cgggaggagc cgggggcggc tggggcatcg ccctccgtgg ccggaggcgc
      421 ggtgggcggc cagtcacggg cgtccgccac aacctcccgc atcttctcga accaccaatg
      481 ggcacgactc gaacggcttc gggcttggcg cgacgtcctc atgcccggcc ggttctcaag
      541 gcctatctgc aattggcggt tcatcaggcg ttacctcccg cttggcctcg gagccgaccg
      601 cagaagaacc gctcggcaca ggccgaaaag acaggcacgc cacgtggcat gcgccgacgc
      661 gtcagccccc gcccgccggg gccacaatga gatcatgcgg aactctaatc tcatacagaa
      721 gcatagcaca aaactcggga caagccagcg ggcttcagtt agtctgcgcc catgaagtca
      781 aaggcgagca ctcgcgctaa gatgcgagtc attcatggcc agccttcctg gcggctggcc
      841 tcaaacgagg tggaagtcta tgtgaccggg acaggcgggc atttggggcc gatcaccttc
      901 gagcgcaaag gccggaagct gcagccgtat tcggtcgccc cgtgggccac ggagaaaccc
      961 gcttgcccga tgccgccgat catccgggtc ctgcgcggcg atttcttctg tttgccgttt
     1021 gggaataaca ccactccgtt tcgcggcgaa aagcatccag tccacggtga gaccgccaat
     1081 gcccgctggc agttccaaac cttggagacc aggcgccgcc gccatttgct gcatctgagc
     1141 ctccgaacga aggttcgccg gggacgggtg gacaagcgga tttggctcgt ggacggccag
     1201 aacgtcgttt actcgcagca cgtggtctcg ggcctgagcg gccgaacgaa tctcgggcat
     1261 catgcgatgc tcaagttccc ggaggcaccg ggcagcgggc ggctgtccac cagccgcttc
     1321 gtgtatggac aggtctttcc gcgagcgttt gaactgccgg aggcgggagg ctactcctgg
     1381 ctaaagccag gggcggcatt cgactcgctg gaggaggttc caaccatcac cgggcggcct
     1441 gccgacctga ccctctatcc ggcccggcgc ggcttcgagg acctggtcat gctggtgaac
     1501 ggcgaccacg tgtctttcgc ctggaccgcg gtgacctttc ccagggaacg gtatgtctgg
     1561 ttcgcgttaa aggacccgcg gatcctgcgc gagacgattc tctggctgtc caacggcggg
     1621 cggcactatc cgccctggag cggtcggcat gtcaacgtga tggggatcga ggaagtaacg
     1681 tcctatttcc acttcggcct ggcggaggct gcccgcaaaa acccaatctc ggcgagaggc
     1741 ttcccgacgt gcctggccct gagcccgacc aagccgctgg tggtgcctta tatcatggga
     1801 gtggcggcaa tccctgcggg attcgaccgg gtagcctcga tccgcgccgc gcaagggaat
     1861 caagccattc tcctgaagtc agacagcggc aggcaggtca aggcggcggt agaggtgaac
     1921 ttcctgcgag ccggtgccgg cgtcggcccc ggaaccggct aaaccaatgc tcaataaacc
     1981 aatactcagt ggcaggcggc tacgttaaca tgctcccacc cgcagcaact ccatcaagta
     2041 tgcgctgcac tgcaaccagc cggttgaggg gcattatcgc cggcggcggc atattgttca
     2101 gcattgcctc cgcactgccc gcagccgagg ccggcaccgc cgcaaagtcg caagtccgcg
     2161 tggatgccgg ctttcccggc ggcaatattg tggtggattc catcgtgggc gacacggtga
     2221 cgctgcatca ggacctgcgc gatacggagg gcgactggtt ctactggcac tttcgggtcc
     2281 gtggagcggc agggcggacg ctgcagttct gcttctcgcg ctcgaatccc atcggcaccc
     2341 gcggcccggc actcagccta gacggcggca agtcgtggtc ttggctgggc acaaatgccg
     2401 tccaagggaa gaccttcacc tgcgccgttc cgggcggggc gcgcgaagcg cggttctgct
     2461 tcgcgatccc gtatctggag agcaacctcc aggacttcct gagacgccat cgtcgccacc
     2521 gcaacctgaa ggtggaaacg ctctgccaga cgcccaatgg gcgccgggtg gaactgttgc
     2581 ggttgggccg gcttgatggg aaggccaagc aatgcgtcgt cctggcatgc cgtcatcacg
     2641 cctgcgagat gatcgccagc tacagcctgg aaggcctcat ggaggcggtg ctcgacttcg
     2701 aggaagtccg tccgctgcgc gagcaggtgg agttcttcgt ggtgccgttc atggacaagg
     2761 atggggtcga agccggcgac caggggaaga atcgcaggcc gcacgaccac aatcgcgact
     2821 atcatggcca gagtctttac gcatccgtgc gggcggtgcg ggagttgatc accggccggc
     2881 tcggttcgcg gaacgtcgcc ctcgcactcg acatgcactg cccctacatt cgcggcgcgc
     2941 ataacgagag tatttacttc gtgggcgggc cggaggagca aaactgggcc cgggtgggcg
     3001 agtttgcccg ggtgctcgag gcggtgcagc gcggcccgct tcactatcgg cttgcggata
     3061 acctccccta tggccgggcg tggaacacac tcgaaggacc gccgcgaagc ttcgggcgat
     3121 ggacggccgc gcagccaaac atccgcgtcg gcaccagcat cgaaatcccc tacgcgaacg
     3181 tcggggaaac aaccgtcacg gccgagaatg cgcgcgcctt cggccatgat ctggcccgcg
     3241 ccctggcgca gtatctccca ttgcggattg cggattgagg cgctgcgctc cggcgggtat
     3301 gctcttccag acgttatgag atcctgtgaa cgccaaacct cgccgggccg ccttcaagcc
     3361 gttccccggc cagcctgcgc tcaactcacc ttcctcttcc gtctcccgat tgctttgggc
     3421 ctggttgcgc tttgcggtct gctggtgtcc tgcggcccca agaccgaaca gccgcccgcg
     3481 ccgccggcct cgcagaccaa cgcggcggcg tcggctgcgg cgctgtcgcc ggagctcgcg
     3541 aagctggccg gcaaatggga gcggccggac ggcggctatg tgctggagat caagggcatt
     3601 gatg
//
LOCUS       DAOPQG010000176         3548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_28610_length_3548_cov_16.431148, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000176 DAOPQG010000000
VERSION     DAOPQG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28610_length_3548_cov_16.431148"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            758..1162
                     /locus_tag="PKI07_13430"
     CDS             758..1162
                     /locus_tag="PKI07_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC52083.1"
                     /translation="MKRADLSRKRILLVDDDALVLASTRMLLEVDGHMVVDAATGAQA
                     LEVFAGAEFDLVITDFRMPGMNGGQLTCRIKQAAPGKPVIILTAWPEEVPASVPADMI
                     MSKPYALSELRQAMAHVLGSPPPLSAAGDAAA"
     gene            complement(1191..1565)
                     /locus_tag="PKI07_13435"
     CDS             complement(1191..1565)
                     /locus_tag="PKI07_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52084.1"
                     /translation="MGTDKRIASPSPDFRVMIRNAQGHYLARDDHGLFFTNDRAAAMV
                     FDYLADRVAPQLEELQKTRGIALAVEPVPPEEVYEHCDSCEDLLMPWLIYFDGKRFLC
                     AECRRREHGRARPPRPPRAREK"
     gene            complement(1573..2934)
                     /gene="asnS"
                     /locus_tag="PKI07_13440"
     CDS             complement(1573..2934)
                     /gene="asnS"
                     /locus_tag="PKI07_13440"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOC52085.1"
                     /translation="MRILIKHLLAATEPREAVTVKGWVRTRRDAKGFSFLALNDGSCA
                     ANVQVVVDAGTPGAEQLPQFTTGASAEIEGRLVPSPAAGQKWELRATRVALIGAADAT
                     YPLQKKGHTPEFLRTIAHLRPRSNLFGAMFRLRSRLAFAVHQFFQERDFVYVHTPIIT
                     ASDCEGAGEMFRVTVLPGNRDEPARGDFFGKPAFLTVSGQLEGETFACALSNIYTFGP
                     TFRAENSNTARHAAEFWMIEPEMAFCDLAGDMDLGEEFIQAMARYALEHCAEDLELFA
                     QRVDRHLLARLQFVVERPFVRVSYTDAIRILQQSGRAFEFPVLYGHNLQSEHERFLTE
                     EHFQSPATVFNYPREIKPFYMRLNDDGRTVTAMDVLVPGIGEVIGGAQREERLEQLRA
                     NMAFHRLNPEDYWWYTDLRRYGTVPHAGFGAGFERLLMFLTGMPNIRDVIPFARTPGN
                     AEF"
BASE COUNT          690 a         1202 c         1089 g          567 t
ORIGIN      
        1 ggccattcat cgcggcaagc ctggcactca agccgcaacc ccgctgacgt tgatagactc
       61 ctgaaagcgg gccagaggcc cgcgcgccga cgggtggctc tcctatccgg aacctggatt
      121 tccccggctg aagaagcagc caggacagcc aaagaaccac caacgccgag gtgcacaaat
      181 ccgcccaggc cattcatcgc ggcaagcctg gcactcaagc cgcaaccccg ctgacgttga
      241 tagactcctg aaagcgggcc agaggcccgc gcgccgatga tccagaccga tgccctgccg
      301 tctcaaagcc gccttatgcc ccgtctctcc cggcctgccg agccttgccc ggaaggacgg
      361 gttctccgcc ggctcaatca ccctggagtc tcaacgcaac gactggtctt ggcgagggag
      421 ggcgaagtaa aatgccggag gaaaaagaca atcggcccga tgatgcttgg cggttgcggc
      481 gtccgtgaaa ccggttaacc cgcagaagga cggcgtttcc ggctcttccc cccggccgca
      541 attctggcac aagttctgcg gctattcccc cggtaagtaa gagcagacga ttaccattga
      601 gtgcctttgc ccgcccaagg tgtccgcgtc ataacgatcg cggcaggccc ggcgggcggg
      661 caggattata tgaccagttt tgctgtccat acccggtttt ccggtcgcgc ggcgaaggcc
      721 atccgccttc gcgcaaaagg ccctgcgccc ctggcctatg aaacgagctg acctctcccg
      781 caaacgcatc ctgctggttg atgacgacgc cttggtgctg gcttcgaccc ggatgctgct
      841 ggaggtggac gggcacatgg tggttgacgc cgcgactggc gcccaggcgc ttgaggtctt
      901 cgcaggggcg gagttcgacc tggtgattac cgactttcgg atgcccggaa tgaacggcgg
      961 gcagctcacc tgccggatca agcaggccgc cccggggaag ccggttatca tcctgaccgc
     1021 ctggcccgag gaagtgccgg catcggtccc ggcggacatg attatgtcca agccctacgc
     1081 attaagcgag ctgcggcagg ccatggcgca tgtcctgggt tccccgccgc cgctttccgc
     1141 ggccggagac gccgcggcgt agcgggagcc ggaaaagagc gcggcgccca tcatttttcg
     1201 cgggcgcggg gcgggcgcgg cgggcgggcg cggccgtgtt cgcggcggcg gcattccgcg
     1261 cacagaaacc gttttccgtc gaaatagatc agccacggca tgagcaggtc ttcgcagctg
     1321 tcgcagtgtt catagacttc ctcggggggc accggctcca ccgccagcgc aatcccccgc
     1381 gttttctgca gttcctccaa ctgcggcgcc acgcggtccg ccaggtagtc gaacaccatg
     1441 gccgccgccc ggtcgttggt aaagaacagg ccgtgatcat cccgcgccag gtagtgcccc
     1501 tgcgcgttgc ggatcatcac ccggaagtcc ggggacgggc tggcgatgcg tttatccgtt
     1561 cccatgcggg gctcaaaact cggcgttgcc cggcgtgcgg gcgaagggaa tcacatcccg
     1621 gatattcggc atgcccgtca ggaacatcag cagccgctcg aagccggccc caaacccggc
     1681 atgggggacc gtgccgtagc ggcgcaagtc ggtgtaccac cagtaatcct cggggttcag
     1741 gcggtgaaaa gccatattgg cgcggagctg ttcgaggcgc tcctcgcgct gggcgccgcc
     1801 gatgacttcg ccaatgcccg gcacgagcac gtccatcgcc gtcacggtcc ggccatcgtc
     1861 attcaggcgc atatagaagg gcttgatctc gcgcggatag ttgaacacgg tggccggcga
     1921 ctgaaaatgc tcctcggtca ggaaccgctc gtgctcggat tgcaggttgt gcccatagag
     1981 gacggggaac tcgaacgccc ggcccgactg ctgcaggatc cggatggcgt cggtgtagga
     2041 aacccgcaca aacgggcgct cgacgacgaa ctgaagccgc gcgagcaggt ggcggtccac
     2101 ccgctgggcg aataactcca ggtcttcggc gcaatgctcc agcgcatacc gcgccatggc
     2161 ctggatgaac tcctcgccca ggtccatgtc ccccgccaaa tcgcagaacg ccatctccgg
     2221 ctcaatcatc cagaactcgg ccgcatggcg ggccgtgttc gagttctccg cgcggaaggt
     2281 ggggccgaag gtgtagatgt tggacagcgc gcaggcgaag gtttccccct cgagctggcc
     2341 gctgaccgtg agaaacgccg gcttgccaaa gaaatccccc cgcgccggct catcccggtt
     2401 gccggggagc accgtgacgc ggaacatctc gcccgcgccc tcgcaatcac tggcggtgat
     2461 gatgggggtg tggacataaa caaaatcgcg ctcctggaag aactgatgca cggcaaaggc
     2521 cagccggctg cgcaggcgga acatggcccc aaagagattc gagcgcgggc gcaaatgcgc
     2581 gatggtgcgc aggaattcgg gcgtgtgccc cttcttttgc aacgggtagg tggcatccgc
     2641 cgccccgatc agcgccaccc gcgtcgcccg cagctcccac ttctgccccg ccgcgggcga
     2701 gggcaccagc cgcccctcga tctccgcgga cgcccccgtg gtgaactgcg gcaactgctc
     2761 cgcgcccggc gtgccggcat ccacgacgac ctgcacattg gccgcgcagg agccatcgtt
     2821 caatgccaga aaggagaacc ccttggcgtc ccgccgggtg cgaacccagc ccttgacggt
     2881 gaccgcttcg cgcggctccg tcgctgccag cagatgcttg atcaatatgc gcatggaaac
     2941 gattccaaca accggcgtca gcctagcgca acgcgcccca accgcaagac ccgacccgca
     3001 cggccaaaaa cagggccttg ggttatcggc gtctgagttt cggtagctga ggggaaaacg
     3061 atgtctccaa acccaaagcc gtctttcggg ttcagaaggc cgggatgaac gcgtttcctg
     3121 gttcaaagcc gccggccaac cctgcgggcg tggcggaccg gcgcgagact gccgaaaacg
     3181 cccggcttgc caccccctcg gcgtaacgcc agtgccaccg ttggggattg ccgcggaaga
     3241 aggagcctcc aaacttccat aaggggccgg gccgtgccgt gtcttatcag gcgctcacgc
     3301 gcaggaaccc caaagccctc accgcacagc aagacaccca ctccgaagcg cttaaccgcc
     3361 acccaacccg caaccgcaac cgccccggct cctgaacaac aaaaccaaag tctcagaaca
     3421 ggttcccgaa ccaatcccat ccatcactca ccctgcgcgc aaagcctggg gataaatctt
     3481 caatcaaact catcccaatc agtcgaatac tccgcccacg atgagggcaa cgcaagcagc
     3541 cgcctttc
//
LOCUS       DAOPQG010000177         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_29201_length_3493_cov_15.041303, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000177 DAOPQG010000000
VERSION     DAOPQG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29201_length_3493_cov_15.041303"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..1408
                     /locus_tag="PKI07_13445"
     CDS             479..1408
                     /locus_tag="PKI07_13445"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOC52086.1"
                     /translation="MKNPVVILGRWYSLWWKMRKYPGVYSLSDACTQLRYLLQPQRLT
                     EWELRQWIRKELSIRSESDGVLEIEIKRNGLIFYWLGSLSGGLAMGVRQEMEPSHAHY
                     YNVPPIHLTPASLVLDIGACEGLFAIRVARARQAARIICFEPSARTASFLARAAGKNG
                     VADRIKVEVFAVGRASGEVYFTDSESPEANRVVSTAVGGARKTPQVCLDDYCRDKQIR
                     LGATDLIKVDAEGADLDVIRGAERIIREGAPQIAVTTYHEPAHAGDLIEFLRSLQPGY
                     RFRLKGVTCFRTPDSIRPVLLQAALPLNARAGQ"
     gene            1435..2859
                     /locus_tag="PKI07_13450"
     CDS             1435..2859
                     /locus_tag="PKI07_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52087.1"
                     /translation="MGWNNLVGRLQNSAVVWSWIFNGVRLATGLILLPLVLNKLPAPD
                     LGMYYVLLSLVAIVPLVDFGFGPTIGRFVTYAMGGAETIQAQGVARPGTSTRPNYVLL
                     WQLLFTTRRLYRYLSLALLVVLGAWGTYVVELRIDETSSPLLTRIAWGVTLGAAVVDI
                     YANWWGLYLRSMNEVAAAARIGVLMFAVRLVLAAGLLLAGAGLLALPIGSFFSSLLVR
                     TLARRLCYRLLAGHPPPENIRIKEHLRILWPNTWRMGTQFVSSYLTVNANTAICLHVL
                     GLAANAQYGLSVQLLSIVSGMVAVWTNVRWPLVGQLRARHDLVGVQRLLWPRVWLQTA
                     TFLLACAALLLAGPFLLQHFGGGKQLIPLAWLSLMMLNAFFEMHFNIWGTLLSTENRL
                     PYLWPAVATNVLSFILSLVLIHFTSLGLGALVLGPFLAGILFNHWFWPPYAARTLGTT
                     LFRFLFTGPTTSATAGLAARSKSP"
     gene            complement(3125..3271)
                     /locus_tag="PKI07_13455"
     CDS             complement(3125..3271)
                     /locus_tag="PKI07_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52088.1"
                     /translation="MLNPSVAARVTQQARKRARDRFHPEAVARRHLEIYREVLNRNSQ
                     DAGG"
BASE COUNT          560 a         1035 c         1092 g          806 t
ORIGIN      
        1 gcttcctgcc attccttggg ctggaagttc cagccgcccc caccccaagg gtctgaggga
       61 aggctggcga gcggggtgcc ttcgcgcaag ggcaattttt gagggatggg tgatggcgac
      121 cgggtgcggc acgctacgtg agggtctgca ggctgccggc gcttggaggc tgttcacccg
      181 gcggctgcaa aaaacacctg cgtgcccgcc cggattgtct gcgggaaagc ctgccggcct
      241 tggagggcaa gttcctcacc gccaatctct tgcacggcca gtgcaaactg gcccgaggct
      301 cttgatgcca ggcgcccggc gctgagcgat gcatacgggc gtttcagatg aaagtccgct
      361 ggcgcgggcg ggcaaaaggc gcattagatt ttgacacccg taatggcttg gacaattatc
      421 gagaaccgat gcaaaacgct tgggcttggt tgttcgtgag cggaaaggat gaaaacggat
      481 gaagaatccg gttgttatcc ttgggcgctg gtacagcctg tggtggaaga tgcgaaaata
      541 tcccggggtg tactcgctat cggatgcctg cacgcaatta cgctatcttc tgcagccaca
      601 gcgcttgacg gagtgggagc tgcgccaatg gattcgaaag gagttgagca tccgctccga
      661 gtcggacggc gtgcttgaaa ttgagatcaa acggaacgga ctgatatttt actggctcgg
      721 cagcctgagc ggcgggttgg caatgggggt gcgccaggag atggaaccct cgcatgcgca
      781 ttactacaat gtacccccga tccacctgac cccggcgagc ctggttctgg acatcggggc
      841 gtgcgaagga ctcttcgcga tccgggtcgc ccgagctcgg caggcggcca ggattatctg
      901 cttcgaaccc tctgcccgga cagcttcttt cctggcccgc gctgccggga agaacggggt
      961 tgccgatcgc atcaaggtgg aggtttttgc ggtggggcgc gcctcgggcg aggtttattt
     1021 taccgattcc gagtctccgg aagcgaaccg cgtcgtcagc acggcggttg gcggcgctcg
     1081 caagacgccc caagtatgcc tggatgatta ctgccgggat aaacagatac ggttgggcgc
     1141 caccgatttg atcaaagtgg atgccgaggg cgccgatctc gacgtaatcc gcggcgccga
     1201 acggattatc cgcgaagggg cgccccagat tgcggtgacg acttaccatg agcctgccca
     1261 tgccggtgat ttgattgagt ttctccgttc cctccagccg ggctaccgtt ttcggctcaa
     1321 gggagtcacg tgtttccgca ctccggattc gatccggcca gtgctattgc aggcggcgct
     1381 tcccctcaac gcgcgcgcgg ggcaatgatt gcgcggtagt ttctcatgct gtccatgggc
     1441 tggaataacc tggtgggcag gcttcagaac tccgcggtcg tctggtcctg gatcttcaac
     1501 ggggtccggc tggccacggg gcttattctt ctgccactgg tgctcaacaa gctgcccgcg
     1561 ccggatctgg gcatgtatta tgtgctgcta agcctcgttg cgattgtgcc gctcgtggat
     1621 tttggtttcg ggccaaccat cgggcgattt gtcacttacg cgatgggcgg agcggaaact
     1681 atacaggccc agggggttgc ccgcccgggg acttccactc gcccgaatta tgtcctgctc
     1741 tggcagcttc tcttcaccac gcggcggctc tatcggtatc tcagcctggc gttgttggtg
     1801 gtgttgggcg cctggggcac ctacgtggtg gaattgcgaa ttgatgaaac gtcctccccg
     1861 ttgctcaccc gcatagcctg gggcgtgacg ctcggggccg ccgtggttga catttatgcg
     1921 aattggtggg ggctttacct gcggagcatg aatgaagtgg cggcggctgc ccggattggc
     1981 gtcctgatgt tcgccgtgcg gctcgtgctc gcggcgggac tgttgttagc cggcgcgggt
     2041 ctgttggcgc ttccgatcgg cagtttcttc agcagcctgc tcgtccgcac gctcgcccgc
     2101 cgcctctgct accgccttct cgccgggcac ccgccgccgg aaaacattcg gattaaggag
     2161 catctcagga tcctttggcc gaacacatgg cggatgggga cgcaatttgt cagctcatat
     2221 ctgacggtca acgccaacac cgccatttgc ctgcatgtgc tcggcttggc cgccaacgcc
     2281 cagtacggcc tctcggttca actgctctca attgtcagtg gaatggtggc cgtctggacc
     2341 aatgtccggt ggccccttgt cggccagttg cgcgcgcgcc acgacttggt cggcgtgcag
     2401 cgtctcctct ggccgcgggt ctggctgcag actgcgactt tcctgctcgc ctgtgcggct
     2461 ttgctcctgg cggggccatt ccttctgcag catttcggtg gcggcaaaca actgatcccc
     2521 ctggcgtggt tatcgctgat gatgctcaat gccttcttcg aaatgcactt taatatctgg
     2581 ggcacattgc tttcgacgga gaaccggctg ccctatcttt ggcccgcggt ggctaccaac
     2641 gtgctaagct tcattctgtc cctggtgctg attcacttta cttccctggg cctgggggcg
     2701 ctggtgctgg ggccatttct ggcgggcatc ctgttcaacc attggttttg gcctccttac
     2761 gcggccagaa ctctcgggac caccctcttt cgctttctct ttaccggtcc gactacctcc
     2821 gcaacggctg gtcttgcggc gcgttcgaaa agtccctgaa cttgcatcct cattcatacg
     2881 gccttcggaa gcggttttat tctggcagtt ctctcggccc cctgagtttg tagcgcggcc
     2941 agccgcggat gctcccggca gttctgctgc ggctcttcgg cagccgcaag cccgcatatc
     3001 cggcgcgctg gtttgctctt ccgcttctga acgcgctgcc gggggcgcat ttcaacttcc
     3061 gggctgcggt gatctttgcg ccaagccgcc tgcccttcct tgggcccgtt gccgccgtca
     3121 atcttcagcc gcccgcatcc tgagaattgc ggttcaacac ttcccggtaa atctccaggt
     3181 gccggcgggc aaccgcctcg ggatgaaaac gatcccgcgc ccgcttgcgg gcttgctggg
     3241 tcacgcgcgc cgccactgaa ggattgagca atatttagtt cgcctgctct tgctgccagc
     3301 cgcctgttca gggctctcct ggcgggtggc gctttgcggt ctccaactgg agttgcggaa
     3361 aggttgaacg cagtgataaa gtccggagtc tgaatgaaag catgtctgaa tgaccatttc
     3421 cggtattaga gcccactggc aacgcttgca acagtcggcg gtgttctttg gggtgtttgc
     3481 caccggggtt cgc
//
LOCUS       DAOPQG010000178         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_31050_length_3336_cov_14.612923, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000178 DAOPQG010000000
VERSION     DAOPQG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31050_length_3336_cov_14.612923"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..570)
                     /locus_tag="PKI07_13460"
     CDS             complement(25..570)
                     /locus_tag="PKI07_13460"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOC52089.1"
                     /translation="MSIVTRRGDRGRTSLMYQRPVSKCHARIEACGAVDELNAALGLA
                     RAAARKAPLRRKLAALQQSLVALMGELATLPQDLPRYVKGQYPLLTPSQTAELEQWIR
                     QIEAAPVAPRGWAMPGANTAAATLDLARTVCRRAERRVCALQEARQLTNPEIVIYLNR
                     LSDLLWLLARQAEAKPPPRRS"
     gene            complement(575..1570)
                     /locus_tag="PKI07_13465"
     CDS             complement(575..1570)
                     /locus_tag="PKI07_13465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOC52090.1"
                     /translation="MKSALLSGLALLLALAGAAAAPGVAAAPRCGKVPGLAYVNLSEW
                     ARANGFAGRWTRTNETIELRKPAGRIALTVDSRQIQINGIAVWLFHPVVAQAGAVWLS
                     RLDADLTLQPLLSPPRGSPGARVKTVCLDPGHGGKDPGNQAGANPEKRYTLLLAQEVQ
                     KQLARVGLKGVLTRHSDTFLELPARPALAQRKRADLFVSLHFNGAARNPDTVRGAEVF
                     CLTPAGLASTNADRPEAGANTFIGNRNNSRNLFLAYQVQAALVRNLAAEDRGVRRARF
                     AVLRDAAMPAVLIEAGFMSHPVEGRKILTAAYRQQIARAIVEGLLAYKKAVERPA"
     gene            complement(1642..2961)
                     /locus_tag="PKI07_13470"
     CDS             complement(1642..2961)
                     /locus_tag="PKI07_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOC52091.1"
                     /translation="MLTIPRIWRGCFLLACIGASTALAGPALIITNLPPYGSPANLGG
                     LVQGVPPAEARVAVFIEVPGYGWVTKPTCAQPLTPIQPDGSWSADITTGGTDELATRI
                     AALLVATNYNEPCVLGLDFLPTNVWARALARAVITRPYPGPRWLRFSGYDWWVKKSGP
                     GPVGPGPNYFSDSPDNVWVDAQGRLHLRITHRTNQWQCAELVSARTFGYGSYRFQLES
                     PADDLDPNAVLGLFTWSDDPAYAYREIDIECARWGNAADPNNAQFVVQPYDQPGHLAR
                     LAVPAGQTNAALLFTWETNRVRFQAQRGRFSPQPAPGAVITNWTCSLGVPQTGDENVR
                     LNLWLFNGQAPARQQEVEVIISQFQFVPLDAPRSGLLTNATRLPGGSCRFTLEGDFDR
                     WYQVLASSDLNAWQELATILATNRSLNFEDSAAAGFARRFYRVLTLP"
     gene            complement(3140..3265)
                     /locus_tag="PKI07_13475"
                     /pseudo
     CDS             complement(3140..3265)
                     /locus_tag="PKI07_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          530 a         1029 c         1240 g          537 t
ORIGIN      
        1 tggctgggaa cagccggcgt cgggctatga gcggcgggga ggcggcttgg cttcggcctg
       61 gcgcgccagc agccagagca ggtcggacag ccggttgagg tagatcacga tttcagggtt
      121 ggtgagctgg cgggcttcct gcagggcgca gacgcgtcgt tcggcgcgcc ggcaaacggt
      181 gcgcgcgagg tccagggtgg ccgcggcggt attggcgccc ggcatcgccc agccccgggg
      241 ggcaaccggc gccgcctcga tttgccggat ccattgctcc agttcggcgg tttggctcgg
      301 tgtcaggagc ggatattggc ctttcacata gcgcggcagg tcctggggga gggtggccag
      361 ttcgcccatc aacgcgacca ggctctgctg gagggcggcc agtttgcgcc gcagcggcgc
      421 tttccgggcg gcggcccggg ccaggccgag ggcggcgttt aattcgtcca ccgcgccgca
      481 ggcttcaatg cgcgcgtggc atttggagac cggccgttga tacatcaggg aggtgcggcc
      541 ccgatcgccg cggcgggtga cgatgctcat ggttttaagc cgggcgctcc accgcttttt
      601 tataggccag caggccctca acgatggccc gcgcgatctg ctggcggtag gcggccgtaa
      661 gaatcttgcg cccttccacg gggtgcgaca tgaagcccgc ctcgatcagc accgctggca
      721 tggcggcatc gcgcagcacg gcaaagcgcg cgcgccgcac gccgcggtct tcggcggcga
      781 gattgcggac cagggccgcc tgcacctggt aggcgaggaa gaggttccgg gagttgttcc
      841 ggttgccgat aaaggtattg gcgccggcct ccgggcggtc ggcattggtc gaggccaggc
      901 ccgcgggcgt caggcagaag acttcggcgc cgcgcacggt gtccgggttg cgggcggcgc
      961 cgttgaagtg caggctgacg aacaggtcgg cccgcttgcg ttgcgccagc gccgggcggg
     1021 ccgggagttc gaggaaagtg tcgctgtggc gcgtgagcac ccctttcagc ccgacccgcg
     1081 caagctgctt ttgcacttcc tgcgccagca acaaggtgta gcgtttttcc gggtttgcgc
     1141 cggcctgatt gccggggtct ttgccgccgt ggccggggtc gaggcagacc gttttcaccc
     1201 gggcgccggg ggagccgcgc ggcggcgaca gcaggggctg gagggtgagg tccgcgtcca
     1261 gccgggacag ccataccgcg cccgcctggg ccacgaccgg gtggaaaagc cagacggcga
     1321 tgccgttgat ctggatttgg cgggaatcca ccgtcagggc gattcggccc gcgggtttgc
     1381 gcagctcgat cgtctcattg gtccgggtcc aacggccggc aaagccgttg gcccgggccc
     1441 actccgaaag gttgacgtag gccaggcccg ggaccttgcc gcaccggggg gcggcggcaa
     1501 ctccgggcgc tgccgccgcg cccgcgaggg ccagcagcag cgccaggccg gacaagagcg
     1561 ccgatttcat ggggcgcact ctgcccgccg atggcaccgg gggcaagctt cattcgggcg
     1621 cgccgccgcg cccgggccgg gtcagggcag cgtcaggacg cggtagaaac gccgcgcgaa
     1681 gcccgcggcg gcggagtctt cgaagttcag gctgcggttg gtggccagga tggtggcgag
     1741 ctcctgccag gcgttgaggt ccgaagaggc cagcacctgg taccagcgat caaagtcccc
     1801 ctcgagggtg aagcggcagg agccgccggg gaggcgggtg gcgttggtga gcagcccgga
     1861 acggggggcg tccagcggca cgaactggaa ctgggaaatg atgacttcga cctcctgctg
     1921 ccgggcgggc gcctggccgt tgaagagcca gaggttgagg cggacgtttt catcgccggt
     1981 ctgaggcacg ccgagcgagc aggtccagtt ggtgatgacc gcgcccgggg ccggttgggg
     2041 cgagaagcgg ccgcgctggg cctgaaaccg cacgcggttg gtctcccacg tgaagagaag
     2101 cgcggcgttg gtctgcccgg cgggcaccgc cagccgggcg aggtggccgg gctgatcata
     2161 gggctgcacg acgaactggg cgttattggg gtcggcagcg ttgccccagc gggcgcactc
     2221 gatatcaatc tcgcggtagg cgtaggcggg atcgtcgctc caggtgaaga ggccgagcac
     2281 ggcgttgggg tcgaggtcgt ccgccggaga ctcgagctgg aagcggtagc tgccgtagcc
     2341 gaaggttcgc gcggagacca gttcggcgca ttgccattga ttggtccgat gggtgatccg
     2401 aaggtgcagc cgcccctggg cgtccaccca gacattgtcg gggctgtcgg agaagtagtt
     2461 gggccccggc ccgaccggcc ccggaccgct tttctttacc caccaatcgt aaccggaaaa
     2521 gcggagccag cgcggcccgg gatacgggcg ggtgatgacg gcgcgggcga gggcgcgggc
     2581 ccagacgttg gtgggcagga aatcaaggcc cagcacacac ggctcgttgt aattcgtggc
     2641 gaccagcagg gcggcgatcc gcgtcgccaa ctcgtccgtg ccgccggtgg tgatgtcggc
     2701 gctccaactg ccgtcgggct gaatgggcgt caagggctgc gcgcaggtgg gcttggttac
     2761 ccagccgtag cccggcacct cgataaagac ggcgaccctg gcctcggcgg gcggcactcc
     2821 ctgcaccaga ccgccaagat tcgccggcga gccatacggc ggcaggttgg taataatcag
     2881 ggccggcccg gccagggcag tgctggcgcc aatgcaggcg agcagaaaac agccgcgcca
     2941 aatccgaggg atcgttaaca aacctttgcc aaacatgccc caatatactt ccgttccgcc
     3001 ggatggcaaa ccatcccgaa gtggccgcgc cgggcttttt atcccctccc ctcccggaga
     3061 ttagagcgta ttaaaaatac tgtagccaca accggcaaac cagtttcttg cgtcttgggg
     3121 ggatgaccaa tgccaaagcg gctccgatcg cttcgatcag ggcagcctcg gttcgggcct
     3181 caacctgccg caggaagttc ttgatcttgc tccacctttt ctcgatgggg ttgaggtccg
     3241 gtgagcacgc cggcaagaag aggatctgag cgccctttgc ggtgattaag tcgagggtcg
     3301 gcgcgcgggc gtcccgcccg cttaaatcgt tcgcac
//
LOCUS       DAOPQG010000179         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_32607_length_3212_cov_14.638581, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000179 DAOPQG010000000
VERSION     DAOPQG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32607_length_3212_cov_14.638581"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1601
                     /locus_tag="PKI07_13480"
     CDS             246..1601
                     /locus_tag="PKI07_13480"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC52092.1"
                     /translation="MKNHLPLTRRKFIRTTAASSVALGWLGSGQAPRVLAATADKPAL
                     LGGTPAHQGGWVSWPQWRAEWEPKMLEVWRSRHWFRGSGTHVAEFEKGYAKLLGAKRC
                     LATSSGTTALIVGLHALDVDAGDEVIVSPYTFSASYNAILSLKALPVFADTDPATLTI
                     NPALIESRITERTRAIMPVHIFGMPCEMEAINAIARKHKLAVIEDACQAWLAEYQGRK
                     CGTLGDLGTFSFQESKHLPSGEGGAITSMSEELIDKCDSYHNCGRAVGTNQGTGCFTR
                     GVNYRMTQAQAVLLQLQFDKLVQETEVRRANADYLSANLGKIPGITPVRLPANSRPVW
                     HLYSFRYDASRFNGLSRDQFARAMGAEGVPCGGVYREQYYEGLLDEAIASRGFKRLWS
                     PERLKAYRDSFPELKGNKQVCETTVSFVQSMLLADRRAMDDILEAIRKIQAHSAALAK
                     A"
     gene            1617..3005
                     /locus_tag="PKI07_13485"
     CDS             1617..3005
                     /locus_tag="PKI07_13485"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOC52093.1"
                     /translation="MKLARGFRWMGRGGLALAVALPAIGMAAENTPPAVGLAVRDITP
                     ELPIRLAGYGARNQSAQKVDHPLQAQALALKNPSGERFVLVALDNLEVSRAFMQPVLQ
                     KLAAQLHLGRGEVAVVSSHTHSAPVLDQTLTDMVQPQPEERERIESYSRLLQARLLEL
                     VEAALNDCQPAVFEYGQGRAGFAMNRRVYRGDNVVFGDNPDGPVDWDVPVLRIRGTNG
                     AVRAILFGYACHGTSVRGGEDWYVVSGEYMAYARHHLEAHQPGAIAMYLTGMGADSDP
                     APRGRLLEAKRHGLELAGAVMGVLDRPMRPVRGAFRLAYDEIELPLPAPPSREQLDRD
                     AQGSDVYVKMRAEAYLKRLAAGQSLPESVTLPLAVVRLGNDLTFVLMGGEVVVDYARQ
                     IKRRWAGDHPWPIGYAYEVPCYIPTARIIKEGGYEPDSSLIYYGFYGPLRTSIEAQIL
                     DRLEALMRRTRT"
BASE COUNT          574 a         1006 c         1091 g          541 t
ORIGIN      
        1 tgaagcccta gacgctttcg ccccggcctc ctgagcccga aaagcggttt tgggggtgga
       61 aaaatctttt cccccttacc tgcgggaact caggcgccgg ggccaaaggg tctggggaag
      121 aatcgcaaaa gggcataatt tggggttgtc attatcccgg agctgactca agctcacggg
      181 aaagcaacgg tgataactag tttgatatgc cacaggccga caatacaatg aaaggaagca
      241 atcgtatgaa aaaccatctc cccttgaccc ggcgcaagtt catccgcacc acggctgcga
      301 gctcggttgc cctgggctgg ctgggctcgg gccaggcgcc ccgcgtgctg gcggccacgg
      361 ccgacaaacc ggcgttgctg ggcggcacgc cggcgcacca gggcggatgg gtgagctggc
      421 cgcagtggcg cgcggagtgg gagccgaaaa tgctcgaggt ctggcggagc cggcactggt
      481 ttcgcggttc gggcacgcat gtggccgagt tcgagaaagg ctacgcgaag ctgctgggcg
      541 ccaagcgctg cctggccacc tccagcggca ccacggcgct gatcgttggc ctgcacgccc
      601 tggacgtgga cgccggggat gaggtcatcg tctcgcccta cacgttcagc gccagctaca
      661 atgcgatcct gagcctgaaa gcgctgccgg tgtttgcgga caccgacccg gcgaccctga
      721 cgattaaccc ggccctgatt gagagccgga ttaccgagcg gacgcgggcg atcatgcccg
      781 tgcatatttt cggcatgccg tgcgagatgg aggccatcaa cgcgattgcc cgcaagcaca
      841 agctggccgt gatcgaagat gcctgccagg cctggctcgc ggaataccag ggccggaagt
      901 gcggcaccct gggcgacctg gggacgttca gcttccagga gtccaaacat cttccttccg
      961 gggagggcgg ggcgatcacc agcatgagcg aggagctgat tgataaatgc gactcgtatc
     1021 acaactgcgg gcgcgcggtg ggcaccaacc agggaacggg ctgcttcacc cgcggggtca
     1081 attaccggat gacccaggcg caagcggtgc tgctgcaact gcagtttgac aagctggtgc
     1141 aggaaaccga ggtgcgccgc gcgaacgccg attatttgag cgccaacctc ggcaagattc
     1201 ccggcatcac ccccgtgcgc ctgcccgcca acagccggcc ggtgtggcac ctgtattcat
     1261 tccgctacga tgcgtcccgg ttcaacggcc tgtcgcgcga ccaattcgcc cgggcgatgg
     1321 gggccgaggg ggttccgtgc ggcggcgtgt accgcgaaca atactacgag gggctgctgg
     1381 acgaggcgat tgcttcgcgc ggtttcaaac ggttgtggag ccccgaacgg ctgaaggctt
     1441 accgcgactc gttcccggaa ttaaagggca acaaacaggt ttgcgaaacg acggtgtcct
     1501 tcgtgcagtc catgctgctg gccgatcgcc gcgccatgga cgatatcctc gaggccatcc
     1561 gcaagattca agcccacagc gccgcgctgg cgaaggcgta aacaaccgcg ccgccgatga
     1621 aactcgcccg cggatttcgg tggatggggc gcggcgggct ggcgctggcc gtggcgctgc
     1681 ccgcaatcgg gatggcggcg gagaataccc cgcccgccgt ggggctggcc gtgcgggaca
     1741 tcaccccgga actgccgatc cggctggcgg gatacggcgc ccggaatcaa tccgcgcaga
     1801 aagtggatca cccgctgcag gcccaggcgc tggccctgaa gaacccctcg ggcgagcgtt
     1861 tcgtcctggt ggcgctggat aacctggagg tgagccgcgc cttcatgcag ccggtcttgc
     1921 agaaactggc cgcgcaactg catctgggac gcggcgaagt cgcggtcgtc tccagccaca
     1981 cccattcggc gccggtgctg gaccagaccc tcacggatat ggtgcagccc caaccggagg
     2041 aacgcgagcg catcgaaagc tacagccggc ttttgcaagc ccggttgctc gagttggtgg
     2101 aggcggccct gaacgactgc cagccggcgg ttttcgagta tgggcagggc cgcgccggct
     2161 tcgccatgaa tcggcgcgtc tatcggggcg acaacgtggt gttcggcgat aaccccgatg
     2221 ggccggtgga ttgggatgtg cccgtgctgc gcattcgggg caccaatggc gccgtccgcg
     2281 cgatcctgtt cggctacgcc tgccacggca cctcggtccg ggggggtgag gattggtacg
     2341 tcgtctccgg cgaatatatg gcttacgccc gccatcacct cgaggcgcac cagcccggcg
     2401 ccatcgcgat gtatctcacc ggcatgggcg ccgacagcga tcccgcgccg cgcgggcgtc
     2461 tgctcgaggc caaacggcat ggcctcgaac tggccggcgc cgtcatgggc gtgctggacc
     2521 ggcccatgcg cccggtccgg ggcgcttttc ggctggccta tgatgaaatc gaattgccgc
     2581 tgccagcgcc tcccagccgg gagcaactgg accgggacgc ccagggctcg gacgtttatg
     2641 tgaagatgcg ggcggaggct tatctcaaac ggctggcggc ggggcagtcg ctgcctgaat
     2701 ccgtgaccct gcccctggcc gtcgtgcgcc tcggcaacga cttgaccttt gtgttgatgg
     2761 gcggcgaggt ggtggtggac tacgcgcggc agatcaagcg ccggtgggcg ggcgatcatc
     2821 cgtggcccat cggttatgcc tacgaagtcc cctgctatat ccccaccgcg cgcattatca
     2881 aagagggcgg ctacgaaccg gattcatcac tcatctacta cggtttctac ggccctttgc
     2941 gcaccagtat tgaggcgcag atattggatc ggctggaagc gctgatgcgc cgcacgcgca
     3001 cctgattttg ccgccgccgg ccggcccggg ccgcgccgga gtttgcccgc gggcactggc
     3061 cgcggaactc cccgggaaag ccaaatgacg ggcggacggc aggggccgga ttgtgattgc
     3121 gccacggccc caatgtggga tacttgacat ccgggtaccg gagcgtagct cagcctggca
     3181 gagcacttgg tttgggacca agacgtcgca gg
//
LOCUS       DAOPQG010000180         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_35323_length_3025_cov_14.504714, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000180 DAOPQG010000000
VERSION     DAOPQG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35323_length_3025_cov_14.504714"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="PKI07_13490"
     CDS             <1..1747
                     /locus_tag="PKI07_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOC52094.1"
                     /translation="DFRPDYRRIVRVLRLLPPQVPVICTTATANDRVVQDIESQIASL
                     RILRGPLVRPSLKLYNIKLDSQADRLAWLAHFLPQLPGSGIIYTLTVPDARRAAQWLQ
                     QQGIAARAYHADLEAHERIAAEHALLNNEVKVLVATVALGMGFDKPDLGFVIHFQRPG
                     SVVAYYQQVGRAGRAVDSAFGILLSGREDDEIQQYFIHSAFPPAEVMTGVLEVLGKSG
                     TLTLGQIGAELNFSRGAMEKALKLLEVEGAVEHDQAGYTRTANPWRPDTVRFEQVTQR
                     RYAELAEIKRYVEHPGCLMEFLARALDDPAAAPCGKCMNCSGHRQRRSAPPARVQAAV
                     DFLRHDALELPPRRFWPGPVLAEVYQVLPEAIERYEHGRLKMTIPERLRAQPGRVLCM
                     YGDAGWGREVARGKYETGRFSDALVAAAAELIQSQWQPDPPPQWVTAVPSEHHSELVQ
                     DYARRLGAKLGLPFVAALRKSRGKQPQKQMQNSAQQLRNVLRAFHVADAPDLARPRPE
                     AATAGIRRVFQQVARQVAATFGAAPVLPPVPVLLVDDMVDSGWTLTMAAVLLQRHGSG
                     PVYPFALAKASPRGS"
     gene            1870..2790
                     /locus_tag="PKI07_13495"
     CDS             1870..2790
                     /locus_tag="PKI07_13495"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOC52095.1"
                     /translation="MNVSDYLSDDGLALLALCSAFALPEGAAAGRAAPLTLAEWNKLG
                     RKIHDSPLKQPAALQGCAADVLARELAILPGDAERIVQLLERSGRLALELEGLFSRGL
                     WAVSRVDGLYPKKLRDTLKHQAPTVLFGAGDSQLLGRGGVAVIGSRNIDEAGTAFAEA
                     VGRKIAAARLPVISGGARGTDRLAMSAALEAGRLAVGVLADSLERTVRQPDLRQMLLD
                     GQLALLTPYAPTAGFSVGAAMGRNKVIYGLADFAVVVSSDYQTGGTWAGAVEALKAGW
                     CPVFVRDGAHVSRGNRELLKLGAAACPRGS"
BASE COUNT          474 a          999 c         1029 g          523 t
ORIGIN      
        1 tgatttccgc ccggactacc gccgcatcgt gcgcgtgctg cggttgctgc cgccccaagt
       61 gccggtcatc tgcaccaccg ccaccgccaa cgaccgtgtc gtgcaggata tcgagtcgca
      121 gatcgccagc ctccgcatcc tgcgcggccc actcgtccgc ccctcgctca agctctacaa
      181 tatcaagctg gacagccagg ccgaccggct ggcgtggctg gcgcacttcc tcccccaact
      241 gcccggcagc ggcatcatct acaccctcac ggtgccggat gcccggcggg ccgcccaatg
      301 gctccagcaa cagggcattg ccgcgcgggc ctatcatgcc gacttggaag cccacgagcg
      361 catcgccgcc gaacacgcgc tcctgaacaa cgaagtcaag gtgctggtcg ccacggtcgc
      421 gctgggcatg ggcttcgaca agccggacct cgggttcgtc atccacttcc agcggcccgg
      481 ctcggtggtc gcctattatc agcaggtggg ccgcgccggg cgcgcggtgg attccgcctt
      541 cggcattctc ctgagcgggc gcgaggacga cgaaatccag caatacttca tccacagcgc
      601 cttcccgccg gcggaggtga tgaccggcgt gctcgaagtg ttgggcaagt cggggacgct
      661 gacgctcggc cagattggcg cggagctgaa cttcagccgc ggggcgatgg agaaagcgct
      721 caagctgctg gaagtcgagg gcgccgtgga gcacgaccaa gccggctaca cccgcaccgc
      781 caacccgtgg cgtccggata ccgtccgctt cgagcaggtc acccaacgcc gctacgcgga
      841 actggcggag ataaagcgct acgtggagca ccccggctgc ctgatggagt tcctcgcccg
      901 cgcgctcgat gacccggcgg cggcgccctg cggcaagtgc atgaattgtt ccggccaccg
      961 ccagcgccgc tcggccccgc cggcgcgggt ccaggcggcg gtggacttcc tgcggcatga
     1021 tgcccttgag ttgccgccgc gtcgcttttg gcccgggccc gtgctggcag aggtctatca
     1081 ggtcctgccg gaggcgatcg agcgctacga gcatgggcgg ctcaagatga cgatcccgga
     1141 acggttgcgc gctcagcccg ggcgcgtgct ctgcatgtac ggcgacgccg gctggggccg
     1201 ggaagtcgcg cgtggcaagt atgagacggg ccggttcagt gatgcgttgg tggcggcggc
     1261 ggcagaattg attcagagcc agtggcagcc ggatcctccg ccccaatggg ttacggccgt
     1321 gccgtctgag caccactccg aacttgtgca ggattatgcc cggcggcttg gggcgaagct
     1381 gggtctgcct tttgtcgcgg ccttgcgcaa gagccgcggc aagcagcccc agaagcagat
     1441 gcagaacagc gcccagcaat tacgcaatgt gctccgggcc tttcacgtcg ctgacgcgcc
     1501 tgatttggct cgcccgcgcc ccgaggccgc caccgccgga atcaggcggg tgtttcagca
     1561 agtggcccgg caggtggccg ccaccttcgg cgcggcgcct gtgttgccgc ccgtcccggt
     1621 tctgctggtg gacgacatgg tggattcggg atggacgcta accatggccg cggtattgtt
     1681 gcagcgtcac ggcagcggcc cggtctatcc gtttgccctg gccaaagcgt cgccgcgggg
     1741 gagttgaatg agttaaatgc gaaagtaacg ccgcgggagg gtgcgtggcc tgcgggtcgt
     1801 ggtaggccgc ctgcccttgc gcggcgtaga taagaatgat gctcggtatg atgggttacc
     1861 ttggttgaca tgaacgttag cgattatctc agtgatgatg gtttggcgct gctggcgctg
     1921 tgctcggctt tcgccctgcc cgaaggcgcg gcggcgggca gggcggcccc gctcaccctg
     1981 gctgaatgga acaaactggg gcggaaaatc cacgactcgc cgctcaaaca gcccgccgcg
     2041 ctccagggtt gcgctgccga tgtgttggcc agggagttgg ccattctgcc gggcgacgcg
     2101 gaacgcatcg ttcagttgct ggagcgctcg ggccgcctcg cgcttgagct ggaagggctg
     2161 ttctcgcgcg ggctgtgggc ggtcagccgc gtggacggac tttatccgaa gaagttgcgc
     2221 gacacgctga agcatcaggc gccgacggtg ctgttcggcg caggcgattc tcaactgctc
     2281 ggccggggcg gcgtggcggt gattggctcg cgtaatattg acgaggccgg caccgccttc
     2341 gccgaggccg tcggacgcaa gattgcggcg gcccgtctgc ccgtgatttc cggcggcgcg
     2401 cgcggcaccg accgcctggc catgagcgcc gcgcttgaag ccggtcggct cgccgtcggg
     2461 gtcctggctg acagcctgga gcgcaccgtc cgccagcccg acctgcgcca gatgctgctc
     2521 gatgggcagt tggcgctgct gactccgtat gccccaaccg ccggcttttc cgtcggcgcg
     2581 gcgatgggcc gtaacaaggt tatctacggg ctggcggatt tcgcggtggt ggtaagcagc
     2641 gactatcaga ctggcggcac ctgggccggc gccgtcgagg ccctcaaagc cggctggtgt
     2701 cccgtcttcg ttcgcgacgg cgcccacgtg tccagaggca accgcgaact cctcaagctc
     2761 ggcgccgccg cctgcccgag ggggagttga gccaaatctc agacctgccc ctctggctgg
     2821 ggcagcacgc caggagcaaa ccggtcgaag gagacctgtt cgattaaact ccctgcttaa
     2881 ccggaaacca gccgccacgc ggtttgcaaa ccgcgccgct caatccattc gtcgagcgcg
     2941 ggccggtcta tttgcccgcc ggaaaccgcg atcatagaac ggatgtcacg caggtgcttt
     3001 tccgtgccgc cttcgcggta atacc
//
LOCUS       DAOPQG010000181         2899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_37364_length_2899_cov_9.370605, whole genome shotgun sequence.
ACCESSION   DAOPQG010000181 DAOPQG010000000
VERSION     DAOPQG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37364_length_2899_cov_9.370605"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..2758
                     /locus_tag="PKI07_13500"
     CDS             398..2758
                     /locus_tag="PKI07_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52096.1"
                     /translation="MLSRNHAAPATGAADRSWMEAFTPGRLALVIALFLFVLYPAVIL
                     GAQTFFYQDYGLFTYPNARYLQDCLWRGEVPLWNPLNNCGVPFLAQWNTSVCYPPALV
                     AALLPLPWALNILGLGHLVLAGVGMYLLAWRWTQNRLAASIAGVAFGLNGLLLNCLLW
                     TSNLAALSWQPWVILAVTSAWQRGGGRRLAGAALAGAMQMLSGAPEIILFTWLLLAAL
                     WLRELGRRRVAAGPGLRRFAVVVALVAGLAAVQLLPFRDLVAHSDRDTSYGEADAWPL
                     PAWGWANFIVPQFHAVQSLLDTYHLADQYWTKSYYVGAGVLVLAFVGTWRGRPWQARW
                     LAGAALLGLVLAAGHQGFLYPWLKSVLPAIGFARYPVKFIALPVFALPLLAACGLQAL
                     LDAPAPAARRNLRLLLGTGAFFLILAAGVLVAAHRSPAPGENWAVTCRDGAERMLFLA
                     GVIGGVGGLLAAGQTRTRTLLGAAVVVLIGLDLVGAGRRMHPTATPRVFGPIELNLAS
                     RPRLGESRALVSRQAEAFLNRVGMTNAVAYCATVRGALFHNNNLLENIPKADGFCSLR
                     LRRTTELLAALADTNQLSYSNLLASPLADFLGVAWVTAPDNVFAWQARAGARPLITAG
                     QRPIFADEAQTRAAVTAGDFLPREVVYLPTAARAQVGAAAVPEARILSPQWSAERVRF
                     RVQTPAPALVVIAQSFYPNWRAWVDGCPVRLWRANYACQALEVPAGQHEVSLVHRDRA
                     FTIGAILSALSLGICLVMLWRRGAPDTTPPAAASREREQATVGLTR"
BASE COUNT          387 a          957 c         1021 g          534 t
ORIGIN      
        1 aaatggcgta gtggaaccgc ggattctcca gaaccagggg cgtcgagtcc ccggcgaagg
       61 cggtcgccgc gagaaccgga aggcaaagaa tgaagggtcg tattcgcata gtgcgcacgg
      121 ccgccagcat gatgcccggc agcgcgatcg tcaagccggc gctggagttt tcaacgccca
      181 aatgcccggg tgccggagcc gccagccaac cggaaactcg ccgccgttgc agtgatggcc
      241 ccaagccatg atccgccccc cagccgttat tcccgctcac ccagggcgct ccaggccgcg
      301 gacccgccga cagcctcccg cctcgctcct ttgccgctgc gctggccgct tgccgcttgc
      361 atcgccccgg aggttgcact agcgtctccc ccggctcatg ctgtcgcgaa accatgccgc
      421 ccccgccacg ggcgcggccg atcgttcctg gatggaagcc ttcaccccgg gccggctggc
      481 gctggtgatc gcgttgttcc tgttcgtgct ttaccccgcg gtaatcctcg gcgcgcaaac
      541 tttcttctac caggactacg gcctgttcac ctatcccaac gcgcgctatc tgcaggactg
      601 tctttggcgc ggcgaagtgc cgctctggaa cccgctgaac aactgcggcg tgccgttcct
      661 ggcgcagtgg aatacctcgg tttgctatcc tccggcgctg gtggccgcgc tgctgccgtt
      721 gccgtgggcg ttgaatattc tggggctggg gcatctcgtg ctggcgggcg tggggatgta
      781 tctcctagcg tggcgctgga cgcagaaccg gctggccgcg agcatagccg gcgtggcgtt
      841 tggcttgaac gggctgctgc tcaactgcct gctatggacg agcaacctcg ccgcgctgag
      901 ctggcagccg tgggtgatcc tggcggtgac ctcggcctgg cagcggggcg gaggccggcg
      961 gctggcggga gccgcgctgg ccggggccat gcagatgctg tccggcgcgc ccgagatcat
     1021 cctgttcacc tggctgctgc tggcggcatt gtggctgcga gaattggggc ggcggcgcgt
     1081 ggcggccggg ccggggctgc ggcggtttgc cgtcgtcgtg gcgctggtgg cggggctggc
     1141 cgcggtgcag ttgctgccat tccgggattt ggtggcccat tcggaccggg acacctcgta
     1201 tggcgaggcg gatgcgtggc ccctgccggc ctggggctgg gcgaacttca tcgtgccgca
     1261 gtttcatgcc gtccaatcgc tgttggatac ctaccacctg gccgatcagt attggaccaa
     1321 gtcctattat gtcggcgcgg gcgtgctggt cctggcattc gttggcacct ggcgcgggcg
     1381 cccctggcag gcccgctggc tggcgggcgc ggcgctgctg gggctggtgc tggctgccgg
     1441 gcatcaggga ttcctttacc cctggctgaa aagcgtgttg ccggccattg gcttcgcccg
     1501 ttacccggtc aagttcatcg cgctcccggt ctttgccctt ccgctcctgg cggcgtgcgg
     1561 attgcaggcc ttgctggacg ccccggcccc ggcggcccgg cgaaacctgc gcctcctgct
     1621 gggcacaggg gctttctttt taatcctggc cgcgggcgtg ctggtggccg cgcaccggtc
     1681 gccggcaccg ggtgaaaatt gggcggtgac atgccgggac ggcgcggagc ggatgctgtt
     1741 tctggcgggg gtgatcggcg gcgtgggcgg attgctggca gccggccaaa cccgcacgcg
     1801 caccctgctg ggggcggcgg tcgtggtgct gatcggcctg gacctcgtgg gagcaggccg
     1861 gcgaatgcat ccgacggcga ccccgcgggt ctttgggccg atcgaactga acctggcttc
     1921 ccggccgcgc ctgggagaat cgcgcgcgct ggtcagccga caggcagaag ctttcctcaa
     1981 ccgggtcggc atgaccaacg cggtcgccta ttgcgccacc gtgcgcggcg ccctgtttca
     2041 caacaacaac ctgctggaga acatccccaa agcggatggc ttttgctcgc tgcggctccg
     2101 ccgaaccacc gagctgctgg cagcgctcgc cgacacgaat cagttgtcct attcaaacct
     2161 gctggcgtct cccctggcgg atttcctggg tgtggcctgg gtgacggcgc ccgacaacgt
     2221 ctttgcatgg caggcgcgtg ccggcgcaag gcccctgatc acagccgggc agcgcccgat
     2281 tttcgccgac gaagcgcaga cccgggccgc ggtcactgcc ggcgatttcc tgccccgcga
     2341 agtagtttac ctgccgacgg cggcgcgggc gcaggtgggc gccgccgccg ttccagaggc
     2401 tcgcattctc tctccgcaat ggagcgcgga gcgcgtccgg ttccgcgtcc agactcccgc
     2461 gccggcgctg gtggtgatcg cccagtcgtt ctaccccaac tggcgcgcgt gggtggatgg
     2521 gtgtccggtt cggctctggc gggcgaacta cgcctgccag gctctggaag tgcccgccgg
     2581 acagcatgag gtgagcttgg ttcaccggga tcgggcgttc accatcggag cgatcctatc
     2641 agccctttca ctgggaatct gcctggtgat gctgtggcga cgaggggcac cggacacgac
     2701 accacccgcc gccgcctccc gggagcggga gcaagcaacc gttggcttaa cgcgctaaaa
     2761 agcttgccgt ctgtcgcgaa cgcacggggc cgggatggtc gcgagaggcg ttaatgcgct
     2821 ggagggctgg cgccgggcga atgcgcggcg cggcgctgct cgagttcctc gatttggcgt
     2881 tgcaacgcct ccgggtgag
//
LOCUS       DAOPQG010000182         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_38819_length_2815_cov_14.007609, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000182 DAOPQG010000000
VERSION     DAOPQG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38819_length_2815_cov_14.007609"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..1153
                     /locus_tag="PKI07_13505"
     CDS             341..1153
                     /locus_tag="PKI07_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC52097.1"
                     /translation="MGVNPRRPRGFTLIELLVVIAIIAILAALLLPALARAKSKALAI
                     SCAANMKNWGWATLMYEDDFNDRFPLFGELGTPPYTAPFWFEILAPYVVKRAKTDPNA
                     SFATDPLFYDRLRQCPGGAVGPAPLSGNTPSGTNWNCYIGCYFGGYGDPVTVGAYKGI
                     SGPFYYGNRRPPMPASRIKRPAQAMIFIDTITHYLYSPLAYPFTDDKDGDGSPDTWPN
                     YGVAFNWGRPTVHSGGANITAADGHVERIAYRVLWRLNPANQMPASLFWYME"
     assembly_gap    1396..1495
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1642..2436
                     /locus_tag="PKI07_13510"
     CDS             1642..2436
                     /locus_tag="PKI07_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC52098.1"
                     /translation="MLALKVKFCLRWGRRRAFTLIELLVVIAIIAILAALLLPALAKA
                     KSKANAITCLNNQKQWGLASKMYADENNDYVPQEGNTIAPVNRKPDNVDAWYNTVSVY
                     VKQPSLASLYTATPPAPPLPSTRIIYSCPSAEKPNWEPSVTKAYFMYGENGRLCVNKS
                     SSNEGRSQTKFSQIVYPVDTILFAEVNGNSPTAGAAQSNVTGQYAVAKHEGRGQFAMC
                     DGSARSARTNEFLRTPTESNSCKAEWDPAKMPRRMYWYPTPNTPDS"
     gene            2522..>2815
                     /locus_tag="PKI07_13515"
     CDS             2522..>2815
                     /locus_tag="PKI07_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52099.1"
                     /translation="MKRTLLLLAVCAGCALNAPADLVFTDNFDSYADGPLVSGSGGVW
                     KSHSGTLNQLMVASGQVVLGGANSEDVNAYITNSIFPKPFETGPGNGSLYASFT"
BASE COUNT          544 a          895 c          787 g          489 t
ORIGIN      
        1 ggaggaaggc tggcgggcgg gaggtcttcg cgcaagggca attttttggg gttggcggag
       61 gcggttgggt gcaaagcgcc gcgtgagggc acgcggccta cagggatcgg gatagtgccg
      121 ggggctggtg ggttgccggg agtgcggtgc tgcgggaagg catcttaacc gcaacccagg
      181 tcgccgcctc aacttccccg gctcaagaac gacaaaacca aaggccccgg gcggctggcc
      241 gcacctatcc atttaggggt tgacgcccct tgtcaccccg gttaaaatgc cgaccatgaa
      301 gccgccgttt gtatcgcggc caatcgcagg ggattgggaa atgggcgtca acccgcgccg
      361 cccccggggg ttcacactca tcgaactcct cgtcgtcatc gccattatcg ccatcctcgc
      421 cgcgctcctc ctgcccgcgc tggcccgggc caagtccaag gcgctggcca tcagttgcgc
      481 cgccaatatg aagaactggg gctgggccac gctcatgtat gaagacgatt tcaacgaccg
      541 attcccgttg ttcggcgagc tgggcacgcc gccctatacc gccccgttct ggttcgagat
      601 tctggctccc tacgtcgtca aacgcgccaa gaccgacccc aacgcgtcct ttgccaccga
      661 cccccttttc tatgaccgcc tgcgccagtg cccgggcggg gccgttggcc cggcccccct
      721 ctcgggcaac acgccctcgg gcaccaactg gaattgttac atcggctgct acttcggcgg
      781 ctacggcgac cccgtgaccg tgggcgccta caaaggcatc tccgggccgt tctattacgg
      841 caaccgccgc ccgcccatgc cggcctcgcg aatcaaacgc cccgcccagg ccatgatctt
      901 tattgatacc atcacgcatt acctttactc ccccctcgcc tacccgttca ccgacgacaa
      961 agacggcgat ggctccccgg atacctggcc aaactacggg gtcgccttta actggggacg
     1021 ccccaccgtc cacagcggcg gggccaacat caccgcggcc gacggccacg tcgagcgcat
     1081 cgcctaccgg gtgctctggc ggttgaaccc ggccaaccaa atgccggcca gccttttctg
     1141 gtacatggag taggcgccgg gcccggagga tttggcgcgc gcgcccaaat cgcccgcacc
     1201 aacggcaagc aagccctccg catcgccggc ccggcgtgat cctgcccgcc gcgattggca
     1261 gacgggtttg aagcttcaag gggcgccgcc gccggcggtt gagttccaaa cgccgttcgc
     1321 ctgtcaaggt acgtccaagg cacgggggcc gaggctccgg atgcctggaa aagtcgaagc
     1381 gatgagacgg aaccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagcgc
     1501 gggcgcgtgg aaaaggtcgt ttctgtgaac aactaattcc aagattctat tgccatctcc
     1561 cggggggcac cttacgttag agaaccagat agtgcaaccc ggctgcccgg tggccgccgg
     1621 cccgtatcgt acaatgacaa tatgctcgcg ctgaaagtga agttctgcct tcgttggggt
     1681 cgccggcgtg cgtttacgct gatcgaactg ctggtggtca ttgccattat tgcgattctg
     1741 gcggctttgc tgcttcccgc gctggccaag gctaaatcca aggccaacgc gatcacgtgc
     1801 ttgaacaacc agaagcaatg gggcctggcc tcgaaaatgt atgccgatga aaacaacgac
     1861 tacgtcccgc aggaaggcaa taccattgcg cccgtaaacc ggaagccgga taacgtggat
     1921 gcctggtata atacggtctc ggtgtatgtc aaacagccct cgctggcaag cctctacacg
     1981 gccactccgc ccgcgccgcc gctgccgtcc acccgcatca tctattcctg tcccagcgcg
     2041 gaaaagccca actgggagcc atcggtgacc aaggcgtatt ttatgtatgg cgaaaacggg
     2101 cggctgtgtg tgaacaagtc gtcgagcaac gaggggcgct cgcagaccaa gttcagccag
     2161 attgtttatc ccgtggacac gattctcttt gccgaagtga acggcaattc gcccaccgcg
     2221 ggcgcggccc agtccaacgt gaccggccag tatgcggtcg ccaaacacga ggggcgcggc
     2281 cagtttgcca tgtgcgacgg cagcgcgcgc tcggcccgga ccaacgaatt cctccggacg
     2341 cccacggagt ccaacagttg caaagcggaa tgggatcccg ccaagatgcc ccgccggatg
     2401 tattggtatc ccacccccaa cacgccggat agctgaaccc ttttgcccgc cgaatatctg
     2461 tgaacaaacc ttaaaccgaa tacttcgcga tcccccaaac aaaacaacac ccattaagcc
     2521 tatgaaaaga acactcctcc tcctcgcagt ctgcgccggt tgcgcgctca acgcgccggc
     2581 cgacctggtc tttactgata acttcgatag ctacgcggac ggcccgctcg tctccggatc
     2641 gggcggcgtc tggaagagtc acagtggaac tctcaatcaa ttaatggtcg ccagcggcca
     2701 ggtggtgctg ggaggggcaa acagcgaaga cgttaacgcc tatatcacca attccatctt
     2761 ccccaagccg tttgaaaccg ggccgggcaa tggcagcctc tatgccagtt tcacg
//
LOCUS       DAOPQG010000183         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_43882_length_2566_cov_13.142971, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000183 DAOPQG010000000
VERSION     DAOPQG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43882_length_2566_cov_13.142971"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2094)
                     /locus_tag="PKI07_13520"
     CDS             complement(<1..2094)
                     /locus_tag="PKI07_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC52100.1"
                     /translation="MRLLVFAAAALFAGVAPNSSARTGSAALTFCCSARNDLYLALGR
                     ARCPRFADPLAAIESAAPGSAVLLLADNYPAQPTMLDPRGFDLAQQKQLRLFLEYPAA
                     VPGLELAPPRSTAWERLVVASDRFGPALPRLRILTAHDCQFMPVSNSPPADLILARVA
                     GYDTAVYGISAQEAFPILFEVPGRNLMVATTRLSSFVTGRYAPAGDWLRVWERILSSL
                     DPRHAHALKAVPLVAPAYSATARLPRRFQRAAFNQAAAWFAHSQLLIHPAEQPALYQA
                     LTAGAQTAPPPSPNALLGDGALGLLEGYTSGILPDGNQRRCLALRADCNLESAMVLAL
                     DSQLNRCQRSALIASNLLDFVYFNSGMCQGPRNDPQHGAFGLIGWGDIAPAWLVANYG
                     DDNARAMLATAVASAALRTARWDERLMRALLANLRTTGKLGFRGDRIDLPALNQKGWR
                     PYFEDAPVSYSPHFESYLWACYLWSYQQTGFPLFRDRARMAIRMTMKAYPAQWRWQDN
                     LERAHMLLCLAWLVRVADTPEHRAWLDRVAGDLLAWQQPSGAIQERLGPPHGGHYRAP
                     QSNAEYGTNETPLVQQNGDPVSDQLYTTGFALLGLHEAAGATGDPRFRRAADRLAAYL
                     CRIQTRSQKFPHLSGTWFRAFDDRRWEAWASSADVGWGAWSIESGWGQAWITALLALR
                     EQGVTVWDYTARSGVR"
BASE COUNT          496 a          784 c          909 g          377 t
ORIGIN      
        1 gcgcacaccg cttcgggccg tatagtccca gaccgtcacg ccttgctcgc gcaaagccag
       61 gagggcggtt atccacgcct ggccccagcc gctctcgatg gaccaggcgc cccagccgac
      121 atcggcggaa ctggcccacg cctcccagcg gcggtcgtca aaggcgcgga accaggtgcc
      181 gctcaggtgc gggaacttct gcgagcgggt ttgaatgcgg cacagatagg cggccaaccg
      241 gtccgcggcg cgccggaacc ggggatcgcc ggtggcgcca gcggcttcgt gcaagccgag
      301 cagggcaaag ccggtggtat aaagctggtc gctgacgggg tcgccgttct gctgcacgag
      361 cggggtttcg ttggtgccat attccgcatt tgactgcggg gcgcggtaat ggccgccgtg
      421 gggtgggccg agccgttcct ggatggcgcc ggaaggttgc tgccaggcga ggaggtcgcc
      481 ggcgacgcgg tccagccagg cgcggtgctc gggagtatcc gcgacgcgga cgagccaggc
      541 caggcagaga agcatgtggg cgcgttcgag gttgtcctgc cagcgccact gggcgggata
      601 ggctttcatg gtcatgcgga tggccatgcg cgcgcggtcg cggaacagcg ggaacccggt
      661 ctgctggtag gaccaaaggt agcaggccca aagataggat tcgaaatgag gcgagtagct
      721 gaccggggcg tcctcgaaat aagggcgcca ccccttctga ttcagcgcgg gcaggtcaat
      781 gcgatcgccg cggaatccca gcttgccggt ggtgcggagg ttggcgagca gggcgcgcat
      841 caggcgctca tcccaacgcg ccgttcgcag ggcggcgctg gcgaccgcgg tggccagcat
      901 cgcgcgggca ttatcatccc cgtagttggc caccagccag gccggcgcaa tatcgcccca
      961 gccgatcagg ccaaacgcgc cgtgctgcgg atcattgcgc ggcccctggc acatgcctga
     1021 gttgaaatac acgaagtcca gcaggttgct ggcaatcagg gcactgcgct ggcagcggtt
     1081 gagctggctg tccagtgcca gaaccatggc ggattccagg ttgcaatccg cccgcagcgc
     1141 caggcagcgg cgctggttgc catcggggag gattcccgag gtatagcctt cgagcaggcc
     1201 cagcgcgccg tcacccaaca gcgcgttggg cgatggcggc ggggcggttt gcgctcccgc
     1261 ggtcaaggcc tggtatagcg ccggctgctc cgcgggatgg atcaatagct gtgaatgggc
     1321 aaaccaggcc gccgcctggt tgaaagccgc gcgctgaaag cggcggggaa gccgggcggt
     1381 ggcgctataa gccggcgcaa cgagcggcac cgccttgagc gcgtgcgcgt gccgcggatc
     1441 cagcgaggaa agaatccttt cccacacgcg cagccaatcc ccggccggag cgtagcgccc
     1501 ggtgacgaag ctgcttagcc gggttgtggc gaccatgagg ttgcggccgg gcacctcaaa
     1561 aagaatggga aaggcctcct gggccgagat gccgtagacg gccgtatcat agcccgcaac
     1621 ccgtgcgagg atcaaatcgg cgggcggcga attgctgacc ggcatgaact ggcaatcgtg
     1681 cgcggtgaga atgcggaggc gtggcaacgc cggcccgaac ctgtccgacg caacgacaag
     1741 ccgctcccag gcggtgctgc gcggcggggc cagttccagg ccgggaaccg ccgccgggta
     1801 ttccaggaat aagcggagtt gcttctgctg cgccaggtcg aagccgcgcg ggtcaagcat
     1861 cgtgggctgc gcgggatagt tgtcggccag cagcaagacc gcggaaccgg gagcggcgct
     1921 ttcgatcgcc gccagcggat cggcgaaacg cgggcagcgc gcgcggccca acgcgaggta
     1981 aaggtcattc cgcgcggaac aacaaaacgt cagcgcggcg gagcctgtcc gggcggatga
     2041 attgggggct actccggcaa acagcgccgc cgcggcgaaa acaaggagac gcacgggcca
     2101 tagcatgacc acaagatgta gaaagcccgg aacgaggaca aggccgcgga tgcgaaaaaa
     2161 gcggggtgac ggcgtgggaa cgggcgatgg ctgcggttcg ggttggaaaa gcgcggcgca
     2221 gcaccccatt gaacgccaaa tgcccgcccg ccgctgataa gcctcgtccc cggaggaggc
     2281 cgtccttggg ccgaggcacg ccgggcaccg cttaaagccc gattctgtag ccggaacgat
     2341 ctcgatttta accctaattc gcccccgcga aacacgtaaa tcgcgggttt tacgggcgca
     2401 aggtattcgg aacccagtag tcagcccgtt gaagccattc agaaccaaat ggtccttggg
     2461 gctatccgct tgtctgtcag acccgtttcg acctcagtaa aacgaggacg cgcggttgga
     2521 ctatcccacg gcaacgtttc agataaggca ttcgcaaagc caaaag
//
LOCUS       DAOPQG010000184         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_45705_length_2488_cov_12.872585, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000184 DAOPQG010000000
VERSION     DAOPQG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45705_length_2488_cov_12.872585"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2128)
                     /locus_tag="PKI07_13525"
     CDS             complement(<1..2128)
                     /locus_tag="PKI07_13525"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOC52101.1"
                     /translation="MKINQAVVFCIAVLLFPGVGFPGDLSSLSAEPTIFAEAHELELA
                     EPIDISSDGELLLLFRPGQQGFELVNTQTGQREVVSRTPCTGYFASISPDKKYVCFKD
                     FVMVDGRRLQRPTLYEIAHQSQLPLCEPVFTAGNPVVSARGQIAFTVGPQLIILSPGL
                     DREKEVDLGAVVNVLTFSPDGHRLAFGEVDERISWIEIDSGKRGSVPTTWLHGYQAQF
                     SPDGRALLARSSSGEITACDLSTGEARSFGAARAAAWVDGDTVALVRKSVAHSRVSGT
                     QLLKARLSDGVISTFLTKEGNAELAIKTPVAALVAEDGINLTDARTGAMSKLAVRPLS
                     ADALPDAAKKTLTGTPATGVAKGAKAVVKLAGVPYIHQLYDTADNFPGGASCNATAAL
                     MAIQYYNRLPAHPITCTSGGTHISNYGFYISSIYTYNGHTYNIPSSSAWGSTYAGYYG
                     GFGYFLQDLVGSSLQRSIRLKEWITYHGLSSATDDSVSGETGITKARTEINAGYPVVV
                     LCDITSAGHYLTCIGYLTGQHTLIFNDPYGNKAISYPGWAGAGVYYDWPGYNNGYPNL
                     IGVVRYIYARGTDNAAFVSQTVTNGTTYAPGQSFSCTWTMNNNGTTTWTSSGYSLNYV
                     SGTQMGAPAVNLISANVAPGGNASIPINFTAPATPGSYTVTMRMNNASGAFFGAQVSL
                     TINVANPVPVITVQPASTTRDPGQTAT"
BASE COUNT          554 a          763 c          685 g          486 t
ORIGIN      
        1 acgtggccgt ctgccccgga tcccgggtcg tgctggccgg ctgcaccgtg atgacaggaa
       61 cggggttcgc aacgttgatg gtcaaggaaa cttgcgctcc gaagaaagct ccgctggcat
      121 tgttcatccg catcgtcacc gtataggaac caggcgtcgc gggagcggta aagttgatgg
      181 ggatgctggc attgccgccg ggcgcaacat tggcgctgat taagttgacc gcaggcgccc
      241 ccatctgggt accgcttacg tagttaagcg agtagccgct cgaggtccag gtagtcgtgc
      301 cattattatt catggtccag gtgcacgaaa aactctgacc cggcgcatac gtggtgccgt
      361 tggtgaccgt ttgtgataca aaggcggcat tatcagtgcc ccgggcataa atgtaccgca
      421 ccaccccgat gagattcggg tatccattgt tgtagcccgg ccaatcgtaa tacacgcccg
      481 cccccgccca tcccggatag ctgatcgctt tgttgccata cggatcatta aagatcaacg
      541 tatgctgccc ggtgaggtag ccgatacacg tcaggtaatg tccggcagag gttatatcgc
      601 acaacaccac cacgggataa ccggcgttaa tttccgtccg tgccttggtt atgccggttt
      661 cgcctgagac cgagtcatcc gtggctgaac tcaaaccgtg gtacgtaatc cattctttca
      721 aacgaatgct gcgctgcaac gagctgccaa ccagatcctg aagaaaatag ccgaacccgc
      781 cgtaataccc ggcgtacgtt gaaccccaag ctgaggacga gggaatgttg taggtatgcc
      841 cattataagt gtagatgctc gaaatataaa aaccgtagtt ggagatatgg gttccgccgc
      901 tggtgcatgt aatgggatgc gccggcaacc gattatagta ttggatcgcc atcagcgcgg
      961 cagtagcatt acaactggcg ccgccgggaa agttgtccgc ggtatcgtaa agctggtgga
     1021 tgtaaggcac gccagccagt ttgacgacag ccttggcgcc cttcgccacg cccgtcgcgg
     1081 gcgtacccgt caaggtcttc ttcgcagcgt ccgggagggc atctgccgaa aggggccgca
     1141 cggccaactt tgacattgca cccgtgcgcg cgtcggtcag gttgattcca tcttcggcca
     1201 ccagcgccgc aacaggagtc ttgatggcca actcggcatt tccctccttc gtcaggaaag
     1261 tggagattac gccatccgaa agcctggcct tgagcaactg ggtgccgcta acccgcgaat
     1321 gggccactga cttccggacc agcgccactg tgtccccatc cacccaggcc gccgcccggg
     1381 cggcgccaaa actcctggcc tcccccgtag ataaatcgca ggcggtgatt tcgccactgc
     1441 tgctccgagc cagcaacgcc cgaccatcgg gagaaaactg cgcctggtaa ccgtgcagcc
     1501 aggtcgttgg aacactccca cgctttccgc tatcaatctc aatccacgaa atcctctcat
     1561 ccacctcgcc aaacgccaac cggtgcccgt cgggcgaaaa ggtcagtaca ttgacgacgg
     1621 cgccgaggtc cacttccttc tcgcggtcca aaccaggcga caaaatgatc aattgcggcc
     1681 ccaccgtgaa ggcgatctgg cctcgcgcag agaccaccgg attgccggca gtgaacacgg
     1741 gttcgcagag cggcaactgg ctttggtggg caatttcata aagggtcgga cgttgaagcc
     1801 ggcgtccgtc aaccatcacg aagtctttga aacagacata cttcttatcc ggcgaaatgc
     1861 tggcgaaata accggtgcag ggagtgcggg aaacgacctc gcgttgcccg gtctgcgtat
     1921 taaccaattc aaacccctgc tgccccgggc gaaacagcag cagcaattct ccatcgctcg
     1981 aaatgtcaat cggctcagcc aactccaatt catgcgcctc cgcgaatatc gttggttcgg
     2041 cagaaagaga tgacaggtct cccgggaaac caaccccggg aaacagcagc accgcaatac
     2101 aaaacaccac ggcttgattt atcttcataa cgtttcttag ctaagcaggg agcccgggca
     2161 tccgccgggc gtgcggcatt ggccgtacgt ccgacggcgc taccccccga ccttccaact
     2221 cccaatgcta gaaaggtcag ggagagtgct aacgtgttct ggcgcggcaa acggttccgg
     2281 aatggtctga atcccaaacc gccagcaacc gaaccgccat tctgtttcct taactcacag
     2341 ccatggcggc ccggcagcgg ccgcccgaag gccgccgcct ggccgtctgt tcaacgctaa
     2401 ttgacaatca tgatgcggaa gaagcggctg ggaattcgcc caaattcatc cagcaggctc
     2461 gccaccaccg tcgccgtgct ctccgccg
//
LOCUS       DAOPQG010000185         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiota bacterium isolate AMR_MDS_1528
            NODE_68528_length_1838_cov_15.696018, whole genome shotgun
            sequence.
ACCESSION   DAOPQG010000185 DAOPQG010000000
VERSION     DAOPQG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854733
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiota bacterium (wastewater metagenome)
  ORGANISM  Verrucomicrobiota bacterium
            Bacteria; Verrucomicrobiota.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:30:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,642
            CDSs (with protein)               :: 2,642
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 15 of 20
            Pseudo Genes (internal stop)      :: 2 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Verrucomicrobiota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68528_length_1838_cov_15.696018"
                     /isolate="AMR_MDS_1528"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026799"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..>1838
                     /locus_tag="PKI07_13530"
     CDS             467..>1838
                     /locus_tag="PKI07_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOC52102.1"
                     /translation="MSNTASGSKHLFFVSAGKGSLLRPRHSSRAWLGAALLVALLGGL
                     AGGTARASEYRMFWLDAFHDGFYNQSQVDALLGVPGTPARGRIRDVNCNGVIIQVRKR
                     ADVCYPSGVGEPYMSNLSPANYNALEAIIKAAHDTTGGKKRIEVHCWSVVFKTAKGVV
                     YSQHRDTPTGSLTTFDNYWPTRLGSTTGAENGDGAFDPGHPKCLEYLVNAHMDLVNFE
                     TTAGPDGTDGRIDGIHYDYIRFEANTEGFNPTSVARYNARYGLSGDPSSASEQFKQWR
                     RDQCTAFVRQMYARIQKTRPSVKQSGSFVTWNPSPATSTRAAFQATRPYYDVYSDWDS
                     WMEEGIMDLAVPMTYYNWASLPADYTKWMNFEKDRKFNRQLVVGPGIYLNSLADAIYE
                     LQMTRDASPAGNYAEGFSGYSYAVPYAGGSWAGFTNALVTGVTPTWADIPEMPWKTSP
                     TKAHIMG"
BASE COUNT          325 a          497 c          613 g          403 t
ORIGIN      
        1 aggctggcgg gcgggaggtc ttcgcgcaag ggcaattttt tgggattggt ggtggcgggt
       61 gagtgcagag cggaactggg gctggcggcc cgcattgttt ccgacgctgg cgcccagcga
      121 ggctggtcgg cggcgcggtc aggccgcggt ttgaggttct cttgtgttct ccgatggagt
      181 caacccctgt gccgacgggt ttccgacgtt atccccttgc ccgctcagtt ccagtttttc
      241 cgttcgctcc tccggcaagg cccgggcagc gaaggcggcc cgctttgggg gttattttat
      301 gcttgattct agtgggggga ttagcaataa tgcgccttac gtatgtgctg cttgtaattc
      361 tggtcttcgg atgtgccttg cgggaatggt cccgcggccc gcaacccggg tttcacagtg
      421 agggggcatc ggcggccaaa gcagcggctt aacgacaaaa ccacttatgt caaatactgc
      481 atcgggctcc aagcatctct ttttcgtgtc tgccggcaag ggaagtctcc ttcgtccgcg
      541 ccattcgagc cgggcctggc tgggcgcggc gttattggtt gcgctgcttg gcggcctggc
      601 cggagggacg gcccgggcgt cggagtatcg gatgttctgg ctggatgcgt ttcacgatgg
      661 tttttataac cagagccagg tggacgcgtt gctgggggtt ccgggcacgc ccgcccgggg
      721 gcggattcgc gacgttaatt gcaatggtgt tattatccag gtgcgcaagc gggcggacgt
      781 ttgctatccg tccggcgtgg gcgagccgta tatgtccaat ctatctccgg ccaattacaa
      841 tgcactggag gcgattatca aagcggcgca tgacaccacc ggcggcaaga agcggattga
      901 agtgcattgc tggtcggtgg ttttcaagac ggccaaaggg gtggtttatt cgcagcatcg
      961 cgacacgccg actggcagcc tgacgacgtt tgataattac tggcccacgc gcctgggttc
     1021 gactacgggg gcggaaaatg gcgacggcgc atttgatccc gggcatccca agtgccttga
     1081 atacctggtt aacgcgcata tggacctggt taattttgag acgaccgccg gtccggatgg
     1141 cacggatggg cgcattgacg gcattcacta tgattatatc cggtttgagg ccaatacgga
     1201 gggctttaat cccaccagcg tggcccggta taacgcgcgc tatgggctga gcggcgatcc
     1261 ctcctccgca agcgagcagt tcaagcaatg gcggcgcgac cagtgcaccg cctttgtgcg
     1321 gcagatgtat gcccgcattc agaagacgcg gccctcggtc aaacagtctg gctcctttgt
     1381 gacgtggaat ccttcgccgg cgacctcgac acgggcggcg tttcaggcca cgcgccccta
     1441 ctacgatgtg tactccgact gggatagctg gatggaagag gggatcatgg acctggccgt
     1501 gccgatgacc tactacaact gggccagcct gcctgcggac tacacgaagt ggatgaactt
     1561 tgagaaggac cgcaagttta accggcagtt ggtggtcggc ccgggcatct acctgaattc
     1621 actggcggat gccatttacg agttgcagat gacgcgcgat gcgtcgccgg cggggaacta
     1681 tgccgagggg ttcagcggct attcctacgc ggtgccgtat gccgggggtt cctgggcggg
     1741 tttcacgaat gcgctggtga ccggggtgac gccgacctgg gcagacattc cggagatgcc
     1801 ctggaagacc tcgcccacca aggctcatat aatgggga
//
